@18:07:38.93UTC13-Oct-22
ESP2 v3.41 I2C Gateway v3.20 for core bus @18:07:41.50 Configuration for short puck jake 1/11/21 by brent@mbari.org /home/esp/esp2/type/shallow/jake/configure.rb @18:07:45.44 Polling Can every 10:00 @18:07:45.45 Cleanly exited at 18:07:10UTC13-Oct-22 @18:07:45.49 Loading /home/esp/esp2/mission/DA_WCR_101322.rb mission @18:07:45.53 GoodNews.email "Starting DA_WCR_101322 w/pucks in tube 2\n{}\nCTD data unavailable\nCan@18:07:45, 16% flow, 20.6C, 22% humidity, 14.9psia, 13.764V, 0.315A, 0.302A avg, 4.33W",Subject: "DA_WCR_101322 begun" @18:07:45.56
Preparing to sleep until + 00:00:05 @18:07:45.62 Asleep til + 00:00:05 @18:07:45.64 Continuing at 18:07 @18:07:45.92 Gate.power :core,:OFF @18:07:45.93 Gate.power :raw,:OFF @18:07:45.94 Shutdown @18:07:50.04 GoodNews.email "Then, in tube 2: initialPurge; da [100, 100] #da and wsr are run simultanelously\n/home/esp/esp2/mission/DA_WCR_101322.rb:14\n{1 => 0,\n 2 => 33,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@18:07:45, 16% flow, 20.6C, 22% humidity, 14.9psia, 13.764V, 0.315A, 0.302A avg, 4.33W",Subject: "Asleep until 18:07" @18:07:50.67
Powering core bus @18:07:51.11 upsync /usr/bin/dbclient: Connection to ESPjake@ESPshore:2323 exited: ssh-rsa host key mismatch for ESPshore ! Fingerprint is sha1!! f3:89:b5:6a:6c:73:12:9e:8d:e9:75:a3:9e:aa:f0:41:d7:0c:b2:cf Expected sha1!! 53:fa:aa rsync: connection unexpectedly closed (0 bytes received so far) [sender] rsync error: error in rsync protocol data stream (code 12) at io.c(226) [sender=3.1.3] @18:07:54.09 upsync /usr/bin/dbclient: Connection to ESPjake@ESPshore:2323 exited: ssh-rsa host key mismatch for ESPshore ! Fingerprint is sha1!! f3:89:b5:6a:6c:73:12:9e:8d:e9:75:a3:9e:aa:f0:41:d7:0c:b2:cf Expected sha1!! 53:fa:aa rsync: connection unexpectedly closed (0 bytes received so far) [sender] rsync error: error in rsync protocol data stream (code 12) at io.c(226) [sender=3.1.3] @18:07:56.18
Gate.power :raw,:ON @18:07:57.15 CS.configure :default @18:07:57.24 PS.configure :default @18:07:57.33 SS.configure :default @18:07:57.42 CC.configure :default @18:07:57.51 PC.configure :default @18:07:57.62 Forearm.configure :loose @18:07:57.71 Elbow.configure :loose @18:07:57.82 SC.configure :loose @18:07:57.90 SE.configure :default @18:07:57.95 CH.coast @18:07:58.00 CH.configure :default @18:07:58.03 PH.coast @18:07:58.08 PH.configure :default @18:07:58.12 All dwarf controllers running firmware version 3.85 @18:07:58.22 Valve.reset @18:07:58.46 Intake.select :close @18:07:58.50 Exhaust.select :close @18:07:58.51 CTV.dial :air @18:07:58.59 PSV.dial :air @18:07:58.64 CSV.dial :waste1 @18:07:58.78 PS.reconfigure :default @18:07:58.81
Forearm.reconfigure :loose @18:07:58.89 Forearm.home.seek :retract @18:07:58.91 PS.home.seek 0ml @18:07:59.13 SSV.dial :spit,avoiding: :exhaust @18:07:59.26 CS.reconfigure :fast @18:07:59.29 SS.reconfigure :default @18:07:59.37 CS.home.seek 0ml @18:07:59.40 SS.home.seek 0ml @18:07:59.63 Hand.close @18:07:59.67
Elbow.jog 1500 @18:08:00.09 Elbow.reconfigure :loose @18:08:00.13 Elbow.home.seek :clear @18:08:03.38 SE.reconfigure :default @18:08:03.42 SE.home.seek :down @18:08:04.11 SC.reconfigure :tight @18:08:04.16 SC.home.seek :initial @18:08:07.19 PS.stop @18:08:23.05
SC.configure :limp @18:08:23.10 Forearm.seek :retract @18:08:23.15 Elbow.seek :hammer @18:08:24.19 Forearm.reconfigure :tight @18:08:24.24 Forearm.seek retracted @18:08:24.65 PC.reconfigure :default @18:08:24.67
CC.reconfigure :default @18:08:24.74 PC.home.seek :open @18:08:24.77
CC.home.seek :open @18:08:28.30 Phase [initialPurge; da [100, 100] #da and wsr are run simultanelously] @18:08:28.31 Notice.email "At 18:08:28 began in tube 2: initialPurge; da [100, 100] #da and wsr are run simultanelously\n/home/esp/esp2/mission/DA_WCR_101322.rb:14\n{1 => 0,\n 2 => 33,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@18:08:28, 15% flow, 20.6C, 22% humidity, 14.9psia, 13.773V, 0.284A, 0.260A avg, 3.92W",Subject: "Started DA_WCR_101322 phase initialPurge; da [100, 100] #da and wsr are run simultanelously" @18:08:28.33
Starting initialPurge @18:08:28.36 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @18:08:28.43 Hand.open @18:08:29.22 Elbow.reconfigure :tight @18:08:29.26 Elbow.seek FlushPuck::Garage @18:08:31.17 Elbow.reconfigure :loose @18:08:31.26 Forearm.reconfigure :loose @18:08:31.30 Forearm.seek :garage @18:08:32.24 Hand.close @18:08:32.39 upsync /usr/bin/dbclient: Connection to ESPjake@ESPshore:2323 exited: ssh-rsa host key mismatch for ESPshore ! Fingerprint is sha1!! f3:89:b5:6a:6c:73:12:9e:8d:e9:75:a3:9e:aa:f0:41:d7:0c:b2:cf Expected sha1!! 53:fa:aa rsync: connection unexpectedly closed (0 bytes received so far) [sender] rsync error: error in rsync protocol data stream (code 12) at io.c(226) [sender=3.1.3] @18:08:32.97
Forearm.seek :retract @18:08:33.95 Elbow.reconfigure :tight @18:08:33.99 Elbow.seek CC @18:08:36.95 Elbow.reconfigure :loose @18:08:37.04 Forearm.reconfigure :tight @18:08:37.09 Forearm.seek CC @18:08:38.80 CC.seek :closed @18:08:43.33 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.29 @18:08:43.39 Hand.open @18:08:44.04 Forearm.reconfigure :loose @18:08:44.09 Forearm.seek :retract @18:08:45.04 Flushing the Collection Valve::Manifold @18:08:45.16 CSV.dial CSR @18:08:46.49 Pulling 5ml of CSR.flush @18:08:46.50 CSR.select :flush @18:08:46.61 CS.reconfigure :default @18:08:46.66 CS.seek 5ml @18:09:00.43 delay 5 seconds @18:09:05.45 CSR.select :air @18:09:05.59 CS.reconfigure :fast @18:09:05.64 CS.seek 7.5ml @18:09:12.30 CSV.dial :waste @18:09:13.55 CS.reconfigure :default @18:09:13.60 CS.seek 0ml @18:09:33.68 Purging external Intake @18:09:33.79 CSV.dial :flush @18:09:34.23 CS.reconfigure :fast @18:09:34.27 CS.seek 10ml @18:09:58.52 delay 5 seconds @18:10:03.54 CSV.dial CBV @18:10:03.57
CBV.dial CSV @18:10:04.31 CTV.dial :intake @18:10:05.10 Intake.select :open @18:10:05.20 CS.seek 0ml @18:10:28.61 Intake.select :close @18:10:28.71 CTV.dial :air @18:10:29.49 Flushing upper manifold @18:10:29.60 CSV.dial :flush @18:10:30.34 CS.seek 5ml @18:10:42.92 delay 5 seconds @18:10:47.94 CSV.dial :air @18:10:48.44 CS.seek 7ml @18:10:54.02 CSV.dial CBV @18:10:54.04
CBV.dial CSV @18:10:55.06 CTV.dial :air @18:10:55.08 CS.seek 0ml @18:11:11.64 delay 7 seconds @18:11:18.66 CS.seek 3ml @18:11:26.67 CSV.dial :waste @18:11:27.12 CS.seek 0ml @18:11:34.90 CSV.dial :flush @18:11:35.34 CS.seek 3ml @18:11:43.33 delay 5 seconds @18:11:48.35 CSV.dial :air @18:11:48.97 CS.seek 5ml @18:11:54.45 CSV.dial :air @18:11:54.47 CS.seek 0ml @18:12:06.67 delay 7 seconds @18:12:13.70 CS.seek 3ml @18:12:21.67 CSV.dial :waste @18:12:22.38 CS.seek 0ml @18:12:30.11 Garaging FlushPuck in Collection Clamp @18:12:30.18 Elbow.reconfigure :tight @18:12:30.22 Elbow.seek CC @18:12:30.54 Elbow.reconfigure :loose @18:12:30.63 Forearm.seek CC @18:12:31.63 Hand.close @18:12:32.23 CC.seek :open @18:12:36.87 Forearm.seek :retract @18:12:37.82 Elbow.reconfigure :tight @18:12:37.86 Elbow.seek :garage @18:12:40.78 Elbow.reconfigure :loose @18:12:40.87 Forearm.reconfigure :tight @18:12:40.91 Forearm.seek :garage @18:12:41.86 Hand.open @18:12:42.91 Forearm.reconfigure :loose @18:12:42.95 Forearm.seek :retract @18:12:43.98 da sampling at most 100ml, wcr at most 100ml @18:12:44.00 DAfans.email "Started da in tube 2 at 18:12:43UTC13-Oct-22\n{1 => 0,\n 2 => 33,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@18:08:28, 15% flow, 20.6C, 22% humidity, 14.9psia, 13.773V, 0.284A, 0.260A avg, 3.92W",Subject: "da sampling at most 100ml, wcr at most 100ml" @18:12:44.04
Loading sh1 puck from tube 2 into Collection Clamp @18:12:44.27 SC.reconfigure :tight @18:12:44.33 SC.seek 2 @18:12:48.10 upsync /usr/bin/dbclient: Connection to ESPjake@ESPshore:2323 exited: ssh-rsa host key mismatch for ESPshore ! Fingerprint is sha1!! f3:89:b5:6a:6c:73:12:9e:8d:e9:75:a3:9e:aa:f0:41:d7:0c:b2:cf Expected sha1!! 53:fa:aa rsync: connection unexpectedly closed (0 bytes received so far) [sender] rsync error: error in rsync protocol data stream (code 12) at io.c(226) [sender=3.1.3] @18:12:51.10
SC.configure :limp @18:12:51.30 Elbow.reconfigure :tight @18:12:51.34 Elbow.seek 2 @18:12:53.27 Forearm.reconfigure :tight @18:12:53.31 Forearm.seek 2 + 450 counts @18:12:54.30 SE.reconfigure :pick @18:12:54.35 SE.seek :beamBreak @18:12:55.35 SE.configure :default @18:12:55.50 Elbow.seek 2 @18:12:55.67 Forearm.seek 2 + 300 counts @18:12:55.87 Forearm.reconfigure :loose @18:12:55.91 Hand.close @18:12:56.49 SE.seek :down @18:12:56.68
Forearm.seek :retract @18:12:57.08 SE.stop @18:12:57.62
Elbow.seek CC @18:12:59.25 Elbow.reconfigure :loose @18:12:59.34 Forearm.reconfigure :tight @18:12:59.38 Forearm.seek CC @18:13:00.80 CC.seek :closed @18:13:05.31 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.27 @18:13:05.32 Hand.open @18:13:06.03 Forearm.reconfigure :loose @18:13:06.07 Forearm.seek :retract @18:13:07.05 collectSample 100ml w/bubble point of 24psi @18:13:07.06 Priming 7ml @18:13:07.07 Intake.select :close @18:13:07.08 Exhaust.select :close @18:13:07.09 SS.hold @18:13:07.29 Calibrating pressure and temperature sensors @18:13:07.32 CTV.dial :air @18:13:07.33 SSV.dial :spit,avoiding: :exhaust @18:13:07.35 SS.reconfigure :fast @18:13:07.39 SS.seek 5ml @18:13:10.26 SSV.dial CBV @18:13:10.28
CBV.dial SSV @18:13:11.64 delay 3.8 seconds @18:13:15.56 SSV.dial :spit @18:13:16.85 SS.seek 0ml @18:13:19.64 SSV.dial CBV @18:13:20.95 CTV.dial :intake @18:13:21.72 SSV.dial CBV @18:13:21.74
CBV.dial SSV @18:13:21.76 Allowing 40s for 7.0ml of remaining 7.0ml @18:13:21.77 Intake.select :open @18:13:23.92 Intake @15.6psia, vacuum from 10.0 to 13.0psi @18:13:23.94 SS.reconfigure :inhaling 2.20ml/s @18:13:23.98 SS.seek 7ml @18:13:30.67 Stroke completed -- waiting for pressure to equalize ... @18:13:30.68 SS.reconfigure :exhale @18:13:30.72 SS.hold @18:13:33.62 Intake.select :close @18:13:33.76 SSV.dial :exhaust @18:13:34.50 Exhaust.select :open @18:13:34.60 SS.seek 0ml @18:13:38.65 Exhaust.select :close @18:13:38.75 Sampling 100.0ml @18:13:38.76 Intake.select :close @18:13:38.77 Exhaust.select :close @18:13:38.78 SS.reconfigure :default @18:13:38.82 SS.hold @18:13:39.01 CTV.dial :intake @18:13:39.02 SSV.dial CBV @18:13:39.05
CBV.dial SSV @18:13:39.78 Allowing 130s for 25.0ml of remaining 100.0ml @18:13:39.79 Intake.select :open @18:13:41.94 Intake @15.7psia, vacuum from 10.0 to 13.0psi @18:13:41.95 SS.reconfigure :inhaling 2.20ml/s @18:13:42.00 SS.seek 25ml @18:13:53.24 Vacuum too high -- waiting up to 40 seconds @18:13:53.25 SS.reconfigure :exhale @18:13:53.29 SS.hold @18:13:54.44 Intake @12.2psia, vacuum from 6.7 to 9.7psi @18:13:54.46 SS.reconfigure :inhaling 1.98ml/s @18:13:54.50 SS.seek 25ml @18:13:59.63 Stroke completed -- waiting for pressure to equalize ... @18:13:59.64 SS.reconfigure :exhale @18:13:59.69 SS.hold @18:14:05.32 Intake.select :close @18:14:05.45 SSV.dial :exhaust @18:14:06.20 Exhaust.select :open @18:14:06.31 SS.seek 0ml @18:14:14.97 Exhaust.select :close @18:14:15.07 SSV.dial CBV @18:14:15.09
CBV.dial SSV @18:14:15.84 Allowing 130s for 25.0ml of remaining 75.0ml @18:14:15.85 Intake.select :open @18:14:18.01 Intake @15.7psia, vacuum from 10.0 to 13.0psi @18:14:18.02 SS.reconfigure :inhaling 1.98ml/s @18:14:18.06 SS.seek 25ml @18:14:29.64 Vacuum too high -- waiting up to 40 seconds @18:14:29.65 SS.reconfigure :exhale @18:14:29.69 SS.hold @18:14:30.31 Intake @12.7psia, vacuum from 7.2 to 10.2psi @18:14:30.33 SS.reconfigure :inhaling 1.79ml/s @18:14:30.37 SS.seek 25ml @18:14:30.45 Vacuum too high -- waiting up to 40 seconds @18:14:30.46 SS.reconfigure :exhale @18:14:30.50 SS.hold @18:14:35.35 Intake @14.3psia, vacuum from 8.8 to 11.8psi @18:14:35.37 SS.reconfigure :inhaling 1.61ml/s @18:14:35.41 SS.seek 25ml @18:14:41.55 Stroke completed -- waiting for pressure to equalize ... @18:14:41.56 SS.reconfigure :exhale @18:14:41.61 SS.hold @18:14:45.05 Intake.select :close @18:14:45.18 SSV.dial :exhaust @18:14:45.93 Exhaust.select :open @18:14:46.04 SS.seek 0ml @18:14:54.98 Exhaust.select :close @18:14:55.08 SSV.dial CBV @18:14:55.10
CBV.dial SSV @18:14:55.85 Allowing 130s for 25.0ml of remaining 50.0ml @18:14:55.86 Intake.select :open @18:14:58.02 Intake @15.7psia, vacuum from 10.0 to 13.0psi @18:14:58.03 SS.reconfigure :inhaling 1.61ml/s @18:14:58.08 SS.seek 25ml @18:15:16.65 Stroke completed -- waiting for pressure to equalize ... @18:15:16.66 SS.reconfigure :exhale @18:15:16.70 SS.hold @18:15:24.14 Intake.select :close @18:15:24.28 SSV.dial :exhaust @18:15:25.03 Exhaust.select :open @18:15:25.13 SS.seek 0ml @18:15:33.82 Exhaust.select :close @18:15:33.92 SSV.dial CBV @18:15:33.97
CBV.dial SSV @18:15:34.68 Allowing 130s for 25.0ml of remaining 25.0ml @18:15:34.69 Intake.select :open @18:15:36.84 Intake @15.7psia, vacuum from 10.0 to 13.0psi @18:15:36.86 SS.reconfigure :inhaling 1.61ml/s @18:15:36.90 SS.seek 25ml @18:15:55.87 Stroke completed -- waiting for pressure to equalize ... @18:15:55.88 SS.reconfigure :exhale @18:15:55.92 SS.hold @18:16:03.37 Intake.select :close @18:16:03.51 SSV.dial :exhaust @18:16:04.26 Exhaust.select :open @18:16:04.37 SS.seek 0ml @18:16:13.18 Exhaust.select :close @18:16:13.28 Sampled 100.0ml @18:16:13.30 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @18:16:13.31 Intake.select :close @18:16:13.36 CSV.dial :waste @18:16:13.43 CS.seek 0ml @18:16:13.51 SSV.dial CBV,avoiding: :exhaust @18:16:13.54 CSV.dial :air @18:16:13.60
CBV.dial SSV @18:16:14.31 CS.seek 10ml @18:16:15.42
CTV.dial :air @18:16:16.20 delay 15 seconds @18:16:31.22 SSV.dial :spit,avoiding: :exhaust @18:16:32.55 CTV.dial CSV,avoiding: :intake @18:16:32.58
SS.seek 0ml @18:16:32.70 SSV.dial CBV,avoiding: :exhaust @18:16:38.82 CBV.dial between SSV and waste2 @18:16:39.20 CTV.dial CSV,avoiding: :air @18:16:39.22
CSV.dial CTV @18:16:39.67 CS.reconfigure :pressurize @18:16:39.72 CS.seek 0ml @18:16:58.04 BubblePoint: Intake @39.4psia, Outlet @15.0psia @18:16:58.05 CBV.dial SSV @18:16:58.37 SS.reconfigure :default @18:16:58.42 SS.seek 10ml @18:17:04.05 delay 15 seconds @18:17:19.18 Evacuated: Intake @17.9psia, Outlet @8.6psia @18:17:19.19 SSV.dial :spit,avoiding: :exhaust @18:17:20.50 SS.reconfigure :exhale @18:17:20.54 SS.seek 0ml @18:17:25.85 SS.reconfigure :fast @18:17:25.89 SS.seek 5ml @18:17:28.76 SSV.dial CBV,avoiding: :exhaust @18:17:28.78
CBV.dial SSV @18:17:30.10 CTV.dial :air,avoiding: :intake @18:17:31.46 CSV.dial CTV @18:17:31.50
SS.seek 10ml @18:17:32.49 CSV.dial :air @18:17:32.94 CS.reconfigure :fast @18:17:32.98 CS.seek 0ml @18:17:34.04
SSV.dial :spit,avoiding: :exhaust @18:17:35.36 SS.reconfigure :exhale @18:17:35.40 SS.seek 0ml @18:17:40.41 SS.reconfigure :fast @18:17:40.45 SS.seek 5ml @18:17:40.76 CS.stop @18:17:43.26 SSV.dial CBV,avoiding: :exhaust @18:17:43.30
CBV.dial SSV @18:17:45.59 SSV.dial :spit,avoiding: :exhaust @18:17:46.92 SS.reconfigure :default @18:17:46.96 SS.seek 0ml @18:17:50.27 SS.stop @18:17:50.31 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @18:17:50.32 Intake.select :close @18:17:50.39 CSV.dial :waste @18:17:50.51 SSV.dial CBV,avoiding: :exhaust @18:17:50.53
CBV.dial SSV @18:17:51.16 CS.seek 0ml @18:17:51.38 CSV.dial :air @18:17:51.84
CTV.dial :air @18:17:51.85 delay 15 seconds @18:17:52.12 CS.seek 10ml @18:18:06.87
SSV.dial :spit,avoiding: :exhaust @18:18:08.17 CTV.dial CSV,avoiding: :intake @18:18:08.20
SS.reconfigure :exhale @18:18:08.29 SS.seek 0ml @18:18:08.74 SSV.dial CBV,avoiding: :exhaust @18:18:16.63 CBV.dial between SSV and waste2 @18:18:17.04 CTV.dial CSV,avoiding: :air @18:18:17.06
CSV.dial CTV @18:18:17.51 CS.reconfigure :pressurize @18:18:17.56 CS.seek 0ml @18:18:38.64 BubblePoint: Intake @38.1psia, Outlet @13.9psia @18:18:38.65 CBV.dial SSV @18:18:38.99 SS.reconfigure :default @18:18:39.03 SS.seek 10ml @18:18:44.71 delay 15 seconds @18:18:59.82 Evacuated: Intake @18.9psia, Outlet @11.1psia @18:18:59.83 SSV.dial :spit,avoiding: :exhaust @18:19:01.15 SS.reconfigure :exhale @18:19:01.19 SS.seek 0ml @18:19:06.55 SS.reconfigure :fast @18:19:06.59 SS.seek 5ml @18:19:09.43 SSV.dial CBV,avoiding: :exhaust @18:19:09.48
CBV.dial SSV @18:19:10.74 CTV.dial :air,avoiding: :intake @18:19:12.09 CSV.dial CTV @18:19:12.12
SS.seek 10ml @18:19:13.12 CSV.dial :air @18:19:13.58 CS.reconfigure :fast @18:19:13.63 CS.seek 0ml @18:19:14.56
SSV.dial :spit,avoiding: :exhaust @18:19:15.85 SS.reconfigure :exhale @18:19:15.90 SS.seek 0ml @18:19:19.13 CS.stop @18:19:21.06
SS.reconfigure :fast @18:19:21.10 SS.seek 5ml @18:19:24.01 SSV.dial CBV,avoiding: :exhaust @18:19:24.03
CBV.dial SSV @18:19:26.34 SSV.dial :spit,avoiding: :exhaust @18:19:27.65 SS.reconfigure :default @18:19:27.69 SS.seek 0ml @18:19:31.00 SS.stop @18:19:31.04 Lysing: Adding 2.0ml AqMeOH @18:19:31.05 CBV.dial between CSV and waste1,avoiding: :DBV @18:19:32.52 CTV.dial CSV @18:19:34.85 CSV.dial CSR @18:19:35.64 Pulling 1ml of CSR.AqMeOH @18:19:35.65 CSR.select :AqMeOH @18:19:35.76 CS.reconfigure :default @18:19:35.80 CS.seek 1ml @18:19:39.20 delay 5 seconds @18:19:44.21 CSR.select :air @18:19:44.36 CS.seek 6ml @18:19:57.94 delay 2 seconds @18:19:59.97 CSV.dial :waste2 @18:20:00.42 CS.reconfigure :fast @18:20:00.46 CS.seek 0ml @18:20:14.88 CSV.dial CSR @18:20:15.39 Pulling 2ml of CSR.AqMeOH @18:20:15.40 CSR.select :AqMeOH @18:20:15.50 CS.reconfigure :default @18:20:15.54 CS.seek 2ml @18:20:21.41 delay 5 seconds @18:20:26.43 CSR.select :air @18:20:26.58 CS.seek 5ml @18:20:35.19 delay 2 seconds @18:20:37.21 CSV.dial CTV @18:20:38.12 CS.seek 1ml @18:20:49.36 Before lysing Collection Puck Heater at 20.3C/38.6psia @18:20:49.38 Heating Puck.from 2, :sh1 in Collection Clamp to 70C for 10:00 @18:20:49.40 CH.seek 70C,8:00 @18:22:37.28 CH.hold 10:00,70C @18:22:37.46 Begun lysing Collection Puck Heater at 68C/47.2psia @18:32:37.63 Ended lysing Collection Puck Heater at 70C/45.9psia @18:32:37.66 CS.seek 0ml @18:32:41.19 CTV.dial between spare and air,avoiding: :intake @18:32:42.30 CSV.dial :air @18:32:42.80 CS.seek 5ml @18:32:56.57 CSV.dial CTV @18:32:57.07 delay 2 seconds @18:32:59.10 CSV.dial :air @18:32:59.62 delay 2 seconds @18:33:01.67 CSV.dial CTV @18:33:02.18 delay 2 seconds @18:33:04.20 CSV.dial :air @18:33:04.70 delay 2 seconds @18:33:06.74 CS.seek 0ml @18:33:20.55 Puck @73.9psia after final lysis pressurization @18:33:20.57 Recovering Lysate into Collection syringe @18:33:20.59 CSV.dial CBV @18:33:21.65 CBV.dial CSV @18:33:22.13 CS.seek 5ml @18:33:35.96 delay 5 seconds @18:33:40.97 CTV.dial :air @18:33:41.59 CS.seek 7ml @18:33:47.51 delay 5 seconds @18:33:52.64 CSV.dial :air2 @18:33:53.15 CS.seek 10ml @18:34:01.65 CSV.dial between air and CTV,avoiding: CBV @18:34:02.84 Lysing Complete @18:34:02.98 Storing sh1 puck in Collection Clamp to tube 1 @18:34:03.30 SC.reconfigure :tight @18:34:03.38 SC.seek 1 @18:34:06.98 SC.configure :limp @18:34:07.34 Elbow.reconfigure :tight @18:34:07.39 SE.reconfigure :place @18:34:07.63
Elbow.seek CC @18:34:07.76 SE.seek :beamBreak @18:34:08.40
Elbow.reconfigure :loose @18:34:08.58 Forearm.seek CC @18:34:09.65 Hand.close @18:34:10.36 CC.seek :open @18:34:10.77 SE.configure :default @18:34:10.87 SE.seek :place @18:34:15.45
Forearm.seek :retract @18:34:16.48 Elbow.reconfigure :tight @18:34:16.57 Elbow.seek 1 @18:34:18.47 Elbow.reconfigure :limp @18:34:18.57 Forearm.seek 1 @18:34:19.58 Elbow.hold @18:34:19.70 Elbow.reconfigure :tight @18:34:19.79 Hand.open @18:34:20.71 Forearm.force -20 @18:34:21.85 Forearm.seek 1 + 306 counts @18:34:22.37 Forearm.reconfigure :tight @18:34:22.45 Forearm.seek 1 + 450 counts @18:34:22.86 SE.seek :down @18:34:24.11
Forearm.reconfigure :loose @18:34:24.20 Forearm.seek :retract @18:34:25.62 SE.stop @18:34:25.74
Tranferring DA @18:34:25.76 Beginning samplePassC @18:34:25.79 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @18:34:26.22 Elbow.seek FlushPuck::Garage @18:34:28.18 Elbow.reconfigure :loose @18:34:28.36 Forearm.seek :garage @18:34:29.36 Hand.close @18:34:30.21 Forearm.seek :retract @18:34:31.22 Elbow.reconfigure :tight @18:34:31.30 Elbow.seek CC @18:34:34.36 Elbow.reconfigure :loose @18:34:34.59 Forearm.reconfigure :tight @18:34:34.67 Forearm.seek CC @18:34:36.22 CC.seek :closed @18:34:40.85 Collection Clamp onSpring/offSpring for 14/27 samples, on/off torque 2.32 @18:34:41.01 Hand.open @18:34:41.73 Forearm.reconfigure :loose @18:34:41.80 Forearm.seek :retract @18:34:42.78 PSV.dial PSR @18:34:44.41 Pulling 1.5ml of PSR.flush @18:34:44.42 PSR.select :flush @18:34:44.60 PS.seek 1.5ml @18:35:00.76 delay 5 seconds @18:35:05.80 PSV.dial :waste1 @18:35:06.32 PS.seek 0ml @18:35:22.49 PSV.dial PSR @18:35:23.01 PSR.select :air @18:35:23.19 PS.seek 2.5ml @18:35:49.75 PRV.dial CBV,avoiding: :ab2 @18:35:49.84
CBV.dial PRV @18:35:51.65 PSV.dial :mixloop @18:35:52.73 CTV.dial :air,avoiding: :intake @18:35:52.75 PTV.dial :PRVmixing @18:35:54.15 PS.seek 0ml @18:36:20.62 PSV.dial :air @18:36:21.12 PS.seek 2.5ml @18:36:47.51 PSV.dial :mixloop @18:36:48.03 PS.seek 0ml @18:37:14.46 PSV.dial :air @18:37:14.97 PS.seek 2.5ml @18:37:41.34 PSV.dial :mixloop @18:37:41.85 PS.seek 0ml @18:38:08.28 Garaging FlushPuck in Collection Clamp @18:38:08.42 Elbow.reconfigure :tight @18:38:08.49 Elbow.seek CC @18:38:08.93 Elbow.reconfigure :loose @18:38:09.12 Forearm.seek CC @18:38:10.16 Hand.close @18:38:10.86 CC.seek :open @18:38:15.70 Forearm.seek :retract @18:38:16.73 Elbow.reconfigure :tight @18:38:16.80 Elbow.seek :garage @18:38:19.89 Elbow.reconfigure :loose @18:38:20.07 Forearm.reconfigure :tight @18:38:20.15 Forearm.seek :garage @18:38:21.12 Hand.open @18:38:21.84 Forearm.reconfigure :loose @18:38:21.92 Forearm.seek :retract @18:38:22.92 PSV.dial :mixloop @18:38:22.94 PTV.dial :PRVmixing @18:38:22.98 PRV.dial CBV @18:38:23.02
CBV.dial PRV @18:38:23.06 CTV.dial :air,avoiding: :intake @18:38:23.10 Loading lyfil puck from tube 2 into Collection Clamp @18:38:24.57 SC.reconfigure :tight @18:38:24.65 SC.seek 2 @18:38:28.28 SC.configure :limp @18:38:28.67 Elbow.reconfigure :tight @18:38:28.74 Elbow.seek 2 @18:38:30.83 Forearm.reconfigure :tight @18:38:30.91 Forearm.seek 2 + 450 counts @18:38:32.07 SE.reconfigure :pick @18:38:32.15 SE.seek :beamBreak @18:38:33.31 SE.configure :default @18:38:33.60 Elbow.seek 2 @18:38:33.94 Forearm.seek 2 + 300 counts @18:38:34.17 Forearm.reconfigure :loose @18:38:34.25 Hand.close @18:38:34.87 SE.seek :down @18:38:35.26
Forearm.seek :retract @18:38:35.64 SE.stop @18:38:36.29
Elbow.seek CC @18:38:38.02 Elbow.reconfigure :loose @18:38:38.21 Forearm.reconfigure :tight @18:38:38.30 Forearm.seek CC @18:38:39.88 CC.seek :closed @18:38:44.50 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.16 @18:38:44.52 Hand.open @18:38:45.35 Forearm.reconfigure :loose @18:38:45.44 Forearm.seek :retract @18:38:46.45 CTV.dial CSV,avoiding: :intake @18:38:47.85 CSV.dial CTV @18:38:48.34 PS.seek 2.5ml @18:39:14.89 CS.seek 4.5ml @18:39:29.83 delay 8 seconds @18:39:37.84 PSV.dial :air @18:39:38.35 delay 2 seconds @18:39:40.38 PSV.dial :waste2 @18:39:41.19 PS.seek 0ml @18:40:07.64 CBV.dial between PRV and waste1 @18:40:08.03 CSV.dial :air @18:40:08.52 CS.seek 10ml @18:40:23.41 CSV.dial CTV @18:40:23.91 CS.seek 0ml @18:40:50.57 CBV.dial :waste1 @18:40:50.94 delay 5 seconds @18:40:55.96 CTV.dial :air,avoiding: :intake @18:40:57.45 Storing lyfil puck in Collection Clamp to tube 1 @18:40:57.84 SC.reconfigure :tight @18:40:57.92 SC.seek 1 @18:41:01.50 SC.configure :limp @18:41:01.92 Elbow.reconfigure :tight @18:41:02.00 Elbow.seek 1 @18:41:03.93 Forearm.reconfigure :tight @18:41:04.05 Forearm.seek 1 + 450 counts @18:41:05.18 SE.reconfigure :pick @18:41:05.27 SE.seek :beamBreak @18:41:08.40 SE.configure :default @18:41:08.69 Elbow.reconfigure :limp @18:41:08.79 Forearm.reconfigure :loose @18:41:08.85 Forearm.seek 1 @18:41:09.15 Elbow.hold @18:41:09.27 Elbow.reconfigure :tight @18:41:09.46 SE.seek 0.18 Pucks below Top Plate @18:41:11.07 Forearm.force -20 @18:41:11.17 Forearm.seek 1 + 291 counts @18:41:11.63 Forearm.reconfigure :tight @18:41:11.71 Forearm.seek 1 + 450 counts @18:41:11.95 SE.seek 1.18 Pucks below Top Plate @18:41:12.62 Forearm.reconfigure :loose @18:41:12.71 Forearm.seek :retract @18:41:13.70 Elbow.seek CC @18:41:15.52 Elbow.reconfigure :loose @18:41:15.71 Forearm.seek CC @18:41:16.77 Hand.close @18:41:17.49 CC.seek :open @18:41:22.47 Forearm.seek :retract @18:41:23.52 Elbow.reconfigure :tight @18:41:23.61 Elbow.seek 1 @18:41:25.50 Elbow.reconfigure :limp @18:41:25.59 Forearm.seek 1 @18:41:26.62 Elbow.hold @18:41:26.74 Elbow.reconfigure :tight @18:41:26.81 Hand.open @18:41:27.77 Forearm.force -20 @18:41:27.85 Forearm.seek 1 + 303 counts @18:41:28.27 Forearm.reconfigure :tight @18:41:28.36 Forearm.seek 1 + 450 counts @18:41:28.73 SE.seek :down @18:41:30.02
Forearm.reconfigure :loose @18:41:30.13 Forearm.seek :retract @18:41:31.43 SE.stop @18:41:31.50
PSV.dial :air @18:41:32.31 PS.seek 2.5ml @18:41:58.81 delay 1 second @18:41:59.82 PSV.dial :mixloop @18:42:00.31 delay 1 second @18:42:01.34 PSV.dial :air @18:42:01.87 delay 1 second @18:42:02.92 PSV.dial :mixloop @18:42:03.45 delay 1 second @18:42:04.46 PSV.dial :air @18:42:04.99 delay 1 second @18:42:06.02 PS.seek 0ml @18:42:32.34 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @18:42:32.69 Elbow.seek FlushPuck::Garage @18:42:34.53 Elbow.reconfigure :loose @18:42:34.71 Forearm.seek :garage @18:42:35.77 Hand.close @18:42:36.70 Forearm.seek :retract @18:42:37.71 Elbow.reconfigure :tight @18:42:37.80 Elbow.seek PC @18:42:39.33 Elbow.reconfigure :loose @18:42:39.58 Forearm.reconfigure :tight @18:42:39.67 Forearm.seek PC @18:42:41.23 PC.seek :closed @18:42:46.03 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.56 @18:42:46.23 Hand.open @18:42:47.03 Forearm.reconfigure :loose @18:42:47.12 Forearm.seek :retract @18:42:48.12 PRV.dial :air @18:42:48.62 PSV.dial :mixloop @18:42:49.28 PS.seek 0.3ml @18:42:53.29 PSV.dial PSR @18:42:54.48 Pulling 0.4ml of PSR.air @18:42:54.49 PSR.select :air @18:42:54.55 PS.seek 0.7ml @18:43:00.54 delay 3 seconds @18:43:03.59 PSR.select :air @18:43:03.63 PS.seek 2.5ml @18:43:22.83 delay 3 seconds @18:43:25.86 PSV.dial :mixloop @18:43:27.07 PTV.dial :mixing @18:43:27.87 PBV.dial :waste2 @18:43:28.92 PS.seek 0ml @18:43:55.34 Garaging FlushPuck in Processing Clamp @18:43:55.48 Elbow.reconfigure :tight @18:43:55.55 Elbow.seek PC @18:43:55.77 Elbow.reconfigure :loose @18:43:55.95 Forearm.seek PC @18:43:56.99 Hand.close @18:43:57.62 PC.seek :open @18:44:02.48 Forearm.seek :retract @18:44:03.54 Elbow.reconfigure :tight @18:44:03.62 Elbow.seek :garage @18:44:05.08 Elbow.reconfigure :loose @18:44:05.32 Forearm.reconfigure :tight @18:44:05.39 Forearm.seek :garage @18:44:06.47 Hand.open @18:44:07.22 Forearm.reconfigure :loose @18:44:07.31 Forearm.seek :retract @18:44:08.30 Loading da puck from tube 2 into Processing Clamp @18:44:08.76 SC.reconfigure :tight @18:44:08.83 SC.seek 2 @18:44:12.47 SC.configure :limp @18:44:12.93 Elbow.reconfigure :tight @18:44:13.03 Elbow.seek 2 @18:44:15.06 Forearm.reconfigure :tight @18:44:15.14 Forearm.seek 2 + 450 counts @18:44:16.26 SE.reconfigure :pick @18:44:16.35 SE.seek :beamBreak @18:44:17.56 SE.configure :default @18:44:17.86 Elbow.seek 2 @18:44:18.20 Forearm.seek 2 + 300 counts @18:44:18.43 Forearm.reconfigure :loose @18:44:18.53 Hand.close @18:44:19.24 SE.seek :down @18:44:19.69
Forearm.seek :retract @18:44:20.11 SE.stop @18:44:20.72
Elbow.seek PC @18:44:23.48 Elbow.reconfigure :loose @18:44:23.66 Forearm.reconfigure :tight @18:44:23.73 Forearm.seek PC @18:44:25.29 PC.seek :closed @18:44:30.15 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.60 @18:44:30.17 Hand.open @18:44:30.97 Forearm.reconfigure :loose @18:44:31.04 Forearm.seek :retract @18:44:32.09 Heating Puck.from 2, :da in Processing Clamp to 28C for 2:00:00 @18:44:32.13 PH.seek 28C,5:00 @18:45:00.91 PH.hold 2:00:00,28C @18:45:01.03 delay 1:30 @18:46:31.04 PSR.select :air @18:46:31.08 PSV.dial PSR @18:46:32.39 Pulling 0.5ml of PSR.wash @18:46:32.41 PSR.select :wash @18:46:32.58 PS.seek 0.5ml @18:46:38.49 delay 5 seconds @18:46:43.53 PSR.select :air @18:46:43.70 PS.seek 2ml @18:46:59.82 PTV.dial :puck @18:47:01.74 PSV.dial PTV @18:47:02.52 PBV.dial :waste1 @18:47:04.05 PS.seek 1.5ml @18:47:10.05 delay 3 seconds @18:47:13.09 PBV.dial between PSV and waste1 @18:47:13.50 PS.seek 0ml @18:47:29.80 delay 5:00 @18:52:29.84 recoverPSRreagent08 PTV.dial :puck @18:52:29.85 PBV.dial :waste1 @18:52:30.22 delay 30 seconds @18:53:00.24 PBV.dial between PSV and waste1 @18:53:00.59 PSR.select :air @18:53:00.61 PSV.dial PSR @18:53:01.36 PS.reconfigure :fast @18:53:01.41 PS.seek 2.5ml @18:53:25.48 PSV.dial PTV @18:53:26.23 delay 5 seconds @18:53:31.25 PSV.dial PSR @18:53:32.00 delay 5 seconds @18:53:37.02 PSV.dial PTV @18:53:37.76 delay 5 seconds @18:53:42.78 PSV.dial :air @18:53:43.53 PS.seek 0ml @18:54:06.86 PTV.dial :PRVmixing,via: PRV @18:54:08.17 PSV.dial :mixloop @18:54:08.64 PRV.dial :ab1 @18:54:09.39 PS.reconfigure :slow @18:54:09.44 PS.seek 0.15ml @18:54:12.95 delay 2 seconds @18:54:14.97 CBV.dial PRV @18:54:14.99
PRV.dial CBV @18:54:15.54 PS.seek 0.35ml @18:54:20.17 delay 2 seconds @18:54:22.18 CBV.dial between PRV and PSV @18:54:22.49 PRV.dial :air @18:54:22.93 PS.seek 0.9ml @18:54:34.73 PRV.dial :waste2 @18:54:35.47 PS.seek 0.5ml @18:54:44.32 addPMLReagent08 @18:54:44.33 PSV.dial :air @18:54:44.80 PS.reconfigure :default @18:54:44.84 PS.seek 2ml @18:55:01.07 PSV.dial :PML @18:55:01.53 PTV.dial :mixing @18:55:02.28 PBV.dial :waste1 @18:55:02.68 PS.seek 1.85ml @18:55:05.07 PBV.dial between PSV and waste1 @18:55:05.44 PS.seek 0ml @18:55:25.14 delay 2 seconds @18:55:27.15 Beginning samplePassC @18:55:27.17 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @18:55:27.33 Elbow.reconfigure :tight @18:55:27.37 Elbow.seek FlushPuck::Garage @18:55:28.70 Elbow.reconfigure :loose @18:55:28.78 Forearm.seek :garage @18:55:29.72 Hand.close @18:55:30.43 Forearm.seek :retract @18:55:31.47 Elbow.reconfigure :tight @18:55:31.51 Elbow.seek CC @18:55:34.40 Elbow.reconfigure :loose @18:55:34.48 Forearm.reconfigure :tight @18:55:34.53 Forearm.seek CC @18:55:35.70 CC.seek :closed @18:55:40.21 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.36 @18:55:40.28 Hand.open @18:55:41.00 Forearm.reconfigure :loose @18:55:41.04 Forearm.seek :retract @18:55:41.95 PSV.dial PSR @18:55:43.05 Pulling 1.5ml of PSR.flush @18:55:43.06 PSR.select :flush @18:55:43.17 PS.seek 1.5ml @18:55:59.29 delay 5 seconds @18:56:04.31 PSV.dial :waste1 @18:56:05.22 PS.seek 0ml @18:56:21.38 PSV.dial PSR @18:56:21.85 PSR.select :air @18:56:21.96 PS.seek 2.5ml @18:56:48.51 PRV.dial CBV,avoiding: :ab2 @18:56:48.54
CBV.dial PRV @18:56:50.13 PSV.dial :mixloop @18:56:51.17 CTV.dial :air,avoiding: :intake @18:56:51.18 PTV.dial :PRVmixing @18:56:51.94 PS.seek 0ml @18:57:18.35 PSV.dial :air @18:57:18.80 PS.seek 2.5ml @18:57:45.16 PSV.dial :mixloop @18:57:45.62 PS.seek 0ml @18:58:12.01 PSV.dial :air @18:58:12.47 PS.seek 2.5ml @18:58:38.85 PSV.dial :mixloop @18:58:39.33 PS.seek 0ml @18:59:05.69 Garaging FlushPuck in Collection Clamp @18:59:05.77 Elbow.reconfigure :tight @18:59:05.81 Elbow.seek CC @18:59:06.05 Elbow.reconfigure :loose @18:59:06.14 Forearm.seek CC @18:59:07.14 Hand.close @18:59:07.69 CC.seek :open @18:59:12.30 Forearm.seek :retract @18:59:13.33 Elbow.reconfigure :tight @18:59:13.37 Elbow.seek :garage @18:59:16.34 Elbow.reconfigure :loose @18:59:16.43 Forearm.reconfigure :tight @18:59:16.47 Forearm.seek :garage @18:59:17.51 Hand.open @18:59:18.12 Forearm.reconfigure :loose @18:59:18.16 Forearm.seek :retract @18:59:19.12 delayUntil "19:03:27.14" @18:59:19.15 Whole Cell Archive sampling 100ml @18:59:19.17 Loading wcr puck from tube 2 into Collection Clamp @18:59:19.61 Elbow.reconfigure :tight @18:59:19.66 Elbow.seek 2 @18:59:21.63 Forearm.reconfigure :tight @18:59:21.68 Forearm.seek 2 + 450 counts @18:59:22.74 SE.reconfigure :pick @18:59:22.79 SE.seek :beamBreak @18:59:23.99 SE.configure :default @18:59:24.15 Elbow.seek 2 @18:59:24.32 Forearm.seek 2 + 300 counts @18:59:24.51 Forearm.reconfigure :loose @18:59:24.56 Hand.close @18:59:25.20 SE.seek :down @18:59:25.39 Forearm.seek :retract @18:59:25.99 SE.stop @18:59:26.34 Elbow.seek CC @18:59:27.98 Elbow.reconfigure :loose @18:59:28.07 Forearm.reconfigure :tight @18:59:28.11 Forearm.seek CC @18:59:29.32 CC.seek :closed @18:59:33.84 Collection Clamp onSpring/offSpring for 13/27 samples, on/off torque 2.11 @18:59:33.85 Hand.open @18:59:34.94 Forearm.reconfigure :loose @18:59:35.00 Forearm.seek :retract @18:59:35.97 collectSample 100ml w/bubble point of 35psi @18:59:35.98 Priming 7ml @18:59:35.99 Intake.select :close @18:59:36.00 Exhaust.select :close @18:59:36.01 SS.hold @18:59:36.21 Calibrating pressure sensors @18:59:36.22 CTV.dial :air @18:59:36.23 SSV.dial :spit,avoiding: :exhaust @18:59:36.25 SS.reconfigure :fast @18:59:36.30 SS.seek 5ml @18:59:39.19 SSV.dial CBV @18:59:39.22 CBV.dial SSV @18:59:40.55 delay 3.8 seconds @18:59:44.48 SSV.dial :spit @18:59:45.79 SS.seek 0ml @18:59:48.74 SSV.dial CBV @18:59:50.05 CTV.dial :intake @18:59:50.80 SSV.dial CBV @18:59:50.82 CBV.dial SSV @18:59:50.85 Allowing 40s for 7.0ml of remaining 7.0ml @18:59:50.86 Intake.select :open @18:59:53.02 Intake @15.6psia, vacuum from 10.0 to 13.0psi @18:59:53.04 SS.reconfigure :inhaling 2.20ml/s @18:59:53.08 SS.seek 7ml @18:59:58.99 Stroke completed -- waiting for pressure to equalize ... @18:59:59.00 SS.reconfigure :exhale @18:59:59.04 SS.hold @19:00:03.13 Intake.select :close @19:00:03.27 SSV.dial :exhaust @19:00:04.02 Exhaust.select :open @19:00:04.13 SS.seek 0ml @19:00:08.05 Exhaust.select :close @19:00:08.16 Sampling 100.0ml @19:00:08.17 Intake.select :close @19:00:08.18 Exhaust.select :close @19:00:08.19 SS.reconfigure :default @19:00:08.23 SS.hold @19:00:08.42 CTV.dial :intake @19:00:08.44 SSV.dial CBV @19:00:08.46 CBV.dial SSV @19:00:09.22 Allowing 130s for 25.0ml of remaining 100.0ml @19:00:09.24 Intake.select :open @19:00:11.39 Intake @16.2psia, vacuum from 10.0 to 13.0psi @19:00:11.41 SS.reconfigure :inhaling 2.20ml/s @19:00:11.46 SS.seek 25ml @19:00:24.34 Vacuum too high -- waiting up to 40 seconds @19:00:24.35 SS.reconfigure :exhale @19:00:24.39 SS.hold @19:00:25.08 Intake @12.7psia, vacuum from 7.2 to 10.2psi @19:00:25.10 SS.reconfigure :inhaling 1.98ml/s @19:00:25.14 SS.seek 25ml @19:00:28.28 Stroke completed -- waiting for pressure to equalize ... @19:00:28.29 SS.reconfigure :exhale @19:00:28.34 SS.hold @19:00:31.79 Intake.select :close @19:00:31.94 SSV.dial :exhaust @19:00:32.69 Exhaust.select :open @19:00:32.80 SS.seek 0ml @19:00:41.55 Exhaust.select :close @19:00:41.65 SSV.dial CBV @19:00:41.68 CBV.dial SSV @19:00:42.44 Allowing 130s for 25.0ml of remaining 75.0ml @19:00:42.46 Intake.select :open @19:00:44.61 Intake @16.3psia, vacuum from 10.0 to 13.0psi @19:00:44.63 SS.reconfigure :inhaling 1.98ml/s @19:00:44.67 SS.seek 25ml @19:01:00.95 Stroke completed -- waiting for pressure to equalize ... @19:01:00.98 SS.reconfigure :exhale @19:01:01.02 SS.hold @19:01:05.13 Intake.select :close @19:01:05.28 SSV.dial :exhaust @19:01:06.02 Exhaust.select :open @19:01:06.13 SS.seek 0ml @19:01:14.75 Exhaust.select :close @19:01:14.86 SSV.dial CBV @19:01:14.88 CBV.dial SSV @19:01:15.64 Allowing 130s for 25.0ml of remaining 50.0ml @19:01:15.66 Intake.select :open @19:01:17.81 Intake @16.3psia, vacuum from 10.0 to 13.0psi @19:01:17.83 SS.reconfigure :inhaling 1.98ml/s @19:01:17.87 SS.seek 25ml @19:01:34.39 Stroke completed -- waiting for pressure to equalize ... @19:01:34.40 SS.reconfigure :exhale @19:01:34.44 SS.hold @19:01:38.54 Intake.select :close @19:01:38.68 SSV.dial :exhaust @19:01:39.42 Exhaust.select :open @19:01:39.53 SS.seek 0ml @19:01:48.14 Exhaust.select :close @19:01:48.25 SSV.dial CBV @19:01:48.27 CBV.dial SSV @19:01:49.03 Allowing 130s for 25.0ml of remaining 25.0ml @19:01:49.05 Intake.select :open @19:01:51.20 Intake @16.2psia, vacuum from 10.0 to 13.0psi @19:01:51.22 SS.reconfigure :inhaling 1.98ml/s @19:01:51.26 SS.seek 25ml @19:02:07.50 Stroke completed -- waiting for pressure to equalize ... @19:02:07.51 SS.reconfigure :exhale @19:02:07.55 SS.hold @19:02:11.74 Intake.select :close @19:02:11.88 SSV.dial :exhaust @19:02:12.62 Exhaust.select :open @19:02:12.74 SS.seek 0ml @19:02:21.73 Exhaust.select :close @19:02:21.84 Sampled 100.0ml @19:02:21.86 Drying puck before fixing for 20:00 @19:02:21.87 CBV.dial :waste2b @19:02:22.59 delay 1:00 @19:03:22.62 CSV.dial :air @19:03:23.06 CS.reconfigure :fast @19:03:23.11 CS.seek 10ml @19:03:27.15 recoverPRVreagent08 @19:03:27.16 PTV.dial :mixing @19:03:27.91 PBV.dial :waste1 @19:03:28.28 delay 30 seconds @19:03:47.56 Pumping wcr puck from tube 2 up to 35psi... @19:03:47.57 CBV.dial SSV @19:03:48.87 CBV.dial between CSV and waste2b @19:03:49.82 CSV.dial between air and CTV @19:03:50.14 CS.seek 7.301ml @19:03:57.31 CTV.dial CSV,avoiding: :air @19:03:57.34 CSV.dial CTV @19:03:58.13 CS.reconfigure :pressurize @19:03:58.17 CS.seek 0ml @19:03:58.30 PBV.dial between PSV and waste1 @19:03:58.67 PSV.dial :air @19:03:59.15 PS.reconfigure :fast @19:03:59.19 PS.seek 2.5ml @19:04:11.73 CBV.dial :waste2b @19:04:12.06 CS.reconfigure :default @19:04:12.11 CS.seek 0ml @19:04:18.59 delay 1:00 @19:04:23.28 PSV.dial :mixloop @19:04:23.75 delay 2 seconds @19:04:25.77 PSV.dial :air @19:04:26.23 delay 2 seconds @19:04:28.25 PSV.dial :mixloop @19:04:28.72 delay 2 seconds @19:04:30.74 PSV.dial :air @19:04:31.72 PS.seek 0ml @19:04:55.41 washCycleDA #1 of 2 @19:04:55.42 PSR.select :air @19:04:55.43 PSV.dial PSR @19:04:56.79 Pulling 0.5ml of PSR.wash @19:04:56.80 PSR.select :wash @19:04:56.91 PS.reconfigure :default @19:04:56.95 PS.seek 0.5ml @19:05:02.94 delay 5 seconds @19:05:07.96 PSR.select :air @19:05:08.07 PS.seek 2ml @19:05:18.61 CTV.dial :air,avoiding: :intake @19:05:19.95 Fix #1 of 2 (with RNAlater) for 10:00 @19:05:19.96 CSV.dial CSR @19:05:20.46 Pulling 2ml of CSR.RNAlater @19:05:20.47 CSR.select :RNAlater @19:05:20.58 CS.seek 2ml @19:05:24.18 PTV.dial :puck @19:05:24.92 PSV.dial PTV @19:05:25.66 PBV.dial :waste1 @19:05:26.09 PS.seek 1.5ml @19:05:26.74 delay 5 seconds @19:05:31.76 CSR.select :air @19:05:31.87 CS.reconfigure :fast @19:05:31.91 CS.seek 10ml @19:05:32.00 delay 3 seconds @19:05:35.01 PBV.dial between PSV and waste1 @19:05:35.40 PS.seek 0ml @19:05:51.50 delay 2:00 @19:05:51.62 Pumping wcr puck from tube 2 up to 35psi... @19:05:51.63 CBV.dial SSV @19:05:52.92 CBV.dial between CSV and waste2b @19:05:53.86 CSV.dial between air and CTV @19:05:54.47 CS.seek 7.352ml @19:06:01.28 CTV.dial CSV,avoiding: :air @19:06:01.30 CSV.dial CTV @19:06:02.70 CS.reconfigure :pressurize @19:06:02.75 CS.seek 0ml @19:06:17.51 delay 10:00 @19:07:51.52 recoverPSRreagent08 @19:07:51.53 PTV.dial :puck @19:07:51.54 PBV.dial :waste1 @19:07:51.90 delay 30 seconds @19:08:21.93 PBV.dial between PSV and waste1 @19:08:22.29 PSR.select :air @19:08:22.31 PSV.dial PSR @19:08:23.06 PS.reconfigure :fast @19:08:23.11 PS.seek 2.5ml @19:08:47.24 PSV.dial PTV @19:08:48.00 delay 5 seconds @19:08:53.02 PSV.dial PSR @19:08:53.76 delay 5 seconds @19:08:58.78 PSV.dial PTV @19:08:59.53 delay 5 seconds @19:09:04.55 PSV.dial :air @19:09:05.30 PS.seek 0ml @19:09:28.71 washCycleDA #2 of 2 @19:09:28.72 PSR.select :air @19:09:28.74 PSV.dial PSR @19:09:30.09 Pulling 0.5ml of PSR.wash @19:09:30.10 PSR.select :wash @19:09:30.22 PS.reconfigure :default @19:09:30.26 PS.seek 0.5ml @19:09:36.31 delay 5 seconds @19:09:41.33 PSR.select :air @19:09:41.44 PS.seek 2ml @19:09:57.52 PTV.dial :puck @19:09:57.54 PSV.dial PTV @19:09:58.38 PBV.dial :waste1 @19:09:58.80 PS.seek 1.5ml @19:10:04.87 delay 3 seconds @19:10:07.89 PBV.dial between PSV and waste1 @19:10:08.25 PS.seek 0ml @19:10:24.43 delay 2:00 @19:12:24.46 recoverPSRreagent08 @19:12:24.47 PTV.dial :puck @19:12:24.48 PBV.dial :waste1 @19:12:24.84 delay 30 seconds @19:12:54.86 PBV.dial between PSV and waste1 @19:12:55.21 PSR.select :air @19:12:55.23 PSV.dial PSR @19:12:55.96 PS.reconfigure :fast @19:12:56.01 PS.seek 2.5ml @19:13:19.90 PSV.dial PTV @19:13:20.77 delay 5 seconds @19:13:25.79 PSV.dial PSR @19:13:26.52 delay 5 seconds @19:13:31.54 PSV.dial PTV @19:13:32.29 delay 5 seconds @19:13:37.31 PSV.dial :air @19:13:38.07 PS.seek 0ml @19:14:01.40 getPMLReagent08 @19:14:01.41 PRV.dial :air @19:14:01.83 delay 5 seconds @19:14:06.85 PTV.dial :PRVmixing @19:14:08.16 delay 5 seconds @19:14:13.18 PRV.dial :ab2 @19:14:14.08 PTV.dial :PRVmixing @19:14:14.10 PSV.dial :mixloop @19:14:14.56 PS.reconfigure :default @19:14:14.60 PS.seek 0.3ml @19:14:18.46 delay 2 seconds @19:14:20.48 PRV.dial :air @19:14:21.00 PS.seek 0.55ml @19:14:24.36 addPMLReagent08 @19:14:24.37 PSV.dial :air @19:14:24.84 PS.seek 2ml @19:14:40.45 PSV.dial :PML @19:14:40.93 PTV.dial :mixing @19:14:41.69 PBV.dial :waste1 @19:14:42.11 PS.seek 1.85ml @19:14:44.39 PBV.dial between PSV and waste1 @19:14:44.76 PS.seek 0ml @19:15:04.49 delay 2 seconds @19:15:06.50 delay 8:00 @19:16:17.53 CBV.dial :waste2b @19:16:17.86 CS.reconfigure :default @19:16:17.91 CS.seek 0ml @19:16:23.11 delay 1:00 @19:17:23.13 CTV.dial :air,avoiding: :intake @19:17:24.47 Fix #2 of 2 (with RNAlater) for 10:00 @19:17:24.48 CSV.dial CSR @19:17:24.97 Pulling 1.999ml of CSR.RNAlater @19:17:24.99 CSR.select :RNAlater @19:17:25.14 CS.seek 2ml @19:17:31.42 delay 5 seconds @19:17:36.44 CSR.select :air @19:17:36.55 CS.reconfigure :fast @19:17:36.59 CS.seek 10ml @19:17:56.23 Pumping wcr puck from tube 2 up to 35psi... @19:17:56.25 CBV.dial SSV @19:17:57.53 CBV.dial between CSV and waste2b @19:17:58.48 CSV.dial between air and CTV @19:17:59.08 CS.seek 7.391ml @19:18:05.80 CTV.dial CSV,avoiding: :air @19:18:05.82 CSV.dial CTV @19:18:07.21 CS.reconfigure :pressurize @19:18:07.25 CS.seek 0ml @19:18:22.44 delay 10:00 @19:23:06.52 recoverPRVreagent08 @19:23:06.54 PTV.dial :mixing @19:23:06.55 PBV.dial :waste1 @19:23:06.92 delay 30 seconds @19:23:36.94 PBV.dial between PSV and waste1 @19:23:37.31 PSV.dial :air @19:23:37.78 PS.reconfigure :fast @19:23:37.82 PS.seek 2.5ml @19:24:01.97 PSV.dial :mixloop @19:24:02.45 delay 2 seconds @19:24:04.46 PSV.dial :air @19:24:04.93 delay 2 seconds @19:24:06.95 PSV.dial :mixloop @19:24:07.41 delay 2 seconds @19:24:09.43 PSV.dial :air @19:24:09.90 PS.seek 0ml @19:24:33.60 washCycleDA #1 of 3 @19:24:33.61 PSR.select :air @19:24:33.63 PSV.dial PSR @19:24:34.98 Pulling 0.5ml of PSR.wash @19:24:34.99 PSR.select :wash @19:24:35.10 PS.reconfigure :default @19:24:35.14 PS.seek 0.5ml @19:24:41.04 delay 5 seconds @19:24:46.06 PSR.select :air @19:24:46.17 PS.seek 2ml @19:25:02.29 PTV.dial :puck @19:25:03.02 PSV.dial PTV @19:25:03.76 PBV.dial :waste1 @19:25:04.19 PS.seek 1.5ml @19:25:10.10 delay 3 seconds @19:25:13.12 PBV.dial between PSV and waste1 @19:25:13.50 PS.seek 0ml @19:25:29.82 delay 2:00 @19:27:29.85 recoverPSRreagent08 PTV.dial :puck @19:27:29.86 PBV.dial :waste1 @19:27:30.24 delay 30 seconds @19:28:00.25 PBV.dial between PSV and waste1 @19:28:00.63 PSR.select :air @19:28:00.65 PSV.dial PSR @19:28:01.40 PS.reconfigure :fast @19:28:01.44 PS.seek 2.5ml @19:28:22.46 CBV.dial :waste2b @19:28:22.79 CS.reconfigure :default @19:28:22.83 CS.seek 0ml @19:28:25.60 PSV.dial PTV @19:28:26.35 delay 5 seconds @19:28:27.81 delay 1:00 @19:28:31.36 PSV.dial PSR @19:28:32.11 delay 5 seconds @19:28:37.13 PSV.dial PTV @19:28:38.35 delay 5 seconds @19:28:43.37 PSV.dial :air @19:28:44.12 PS.seek 0ml @19:29:07.75 washCycleDA #2 of 3 @19:29:07.76 PSR.select :air @19:29:07.78 PSV.dial PSR @19:29:09.13 Pulling 0.5ml of PSR.wash @19:29:09.14 PSR.select :wash @19:29:09.26 PS.reconfigure :default @19:29:09.30 PS.seek 0.5ml @19:29:15.25 delay 5 seconds @19:29:20.27 PSR.select :air @19:29:20.39 PS.seek 2ml @19:29:27.84 CTV.dial :air,avoiding: :intake @19:29:36.47 PTV.dial :puck @19:29:36.48 PSV.dial PTV @19:29:37.20 PBV.dial :waste1 @19:29:37.64 PS.seek 1.5ml @19:29:43.63 delay 3 seconds @19:29:46.64 PBV.dial between PSV and waste1 @19:29:47.01 PS.seek 0ml @19:30:03.16 delay 2:00 @19:32:03.18 recoverPSRreagent08 @19:32:03.19 PTV.dial :puck @19:32:03.20 PBV.dial :waste1 @19:32:03.56 delay 30 seconds @19:32:33.59 PBV.dial between PSV and waste1 @19:32:33.94 PSR.select :air @19:32:33.95 PSV.dial PSR @19:32:34.71 PS.reconfigure :fast @19:32:34.75 PS.seek 2.5ml @19:32:58.84 PSV.dial PTV @19:32:59.59 delay 5 seconds @19:33:04.61 PSV.dial PSR @19:33:05.36 delay 5 seconds @19:33:10.38 PSV.dial PTV @19:33:11.12 delay 5 seconds @19:33:16.14 PSV.dial :air @19:33:16.89 PS.seek 0ml @19:33:40.34 washCycleDA #3 of 3 @19:33:40.35 PSR.select :air @19:33:40.37 PSV.dial PSR @19:33:41.72 Pulling 0.5ml of PSR.wash @19:33:41.73 PSR.select :wash @19:33:41.84 PS.reconfigure :default @19:33:41.88 PS.seek 0.5ml @19:33:47.87 delay 5 seconds @19:33:52.89 PSR.select :air @19:33:53.00 PS.seek 2ml @19:34:09.18 PTV.dial :puck @19:34:09.19 PSV.dial PTV @19:34:09.92 PBV.dial :waste1 @19:34:10.32 PS.seek 1.5ml @19:34:16.28 delay 3 seconds @19:34:19.29 PBV.dial between PSV and waste1 @19:34:19.66 PS.seek 0ml @19:34:35.74 delay 2:00 @19:36:35.77 recoverPSRreagent08 @19:36:35.78 PTV.dial :puck @19:36:35.79 PBV.dial :waste1 @19:36:36.15 delay 30 seconds @19:37:06.17 PBV.dial between PSV and waste1 @19:37:06.52 PSR.select :air @19:37:06.54 PSV.dial PSR @19:37:07.29 PS.reconfigure :fast @19:37:07.34 PS.seek 2.5ml @19:37:31.36 PSV.dial PTV @19:37:32.12 delay 5 seconds @19:37:37.14 PSV.dial PSR @19:37:37.88 delay 5 seconds @19:37:42.90 PSV.dial PTV @19:37:43.65 delay 5 seconds @19:37:48.67 PSV.dial :air @19:37:49.42 PS.seek 0ml @19:38:12.96 Gate.power :camera,:ON @19:38:12.97 Mixing DA Substrates @19:38:12.98 PSR.select :air @19:38:13.00 PSV.dial PSR @19:38:14.49 Pulling 0.25ml of PSR.sub1 @19:38:14.52 PSR.select :sub1 @19:38:14.71 PS.reconfigure :default @19:38:14.81 PS.seek 0.25ml @19:38:18.19 delay 5 seconds @19:38:23.25 Pulling 0.25ml of PSR.sub2 @19:38:23.26 PSR.select :sub2 @19:38:23.38 PS.seek 0.5ml @19:38:26.80 delay 5 seconds @19:38:31.82 PSR.select :air @19:38:31.93 PS.seek 2ml @19:38:48.11 PTV.dial :puck @19:38:48.13 PSV.dial PTV @19:38:48.86 PBV.dial :waste1 @19:38:49.28 PS.seek 1.5ml @19:38:55.18 delay 3 seconds @19:38:58.19 PBV.dial between PSV and waste1 @19:38:58.57 PS.seek 0ml @19:39:14.68 delay 10 seconds @19:39:24.70 recoverPSRreagent08 @19:39:24.71 PTV.dial :puck @19:39:24.72 PBV.dial :waste1 @19:39:25.08 delay 30 seconds @19:39:55.11 PBV.dial between PSV and waste1 @19:39:55.47 PSR.select :air @19:39:55.49 PSV.dial PSR @19:39:56.26 PS.reconfigure :fast @19:39:56.30 PS.seek 2.5ml @19:40:20.67 PSV.dial PTV @19:40:21.44 delay 5 seconds @19:40:26.46 PSV.dial PSR @19:40:27.21 delay 5 seconds @19:40:32.23 PSV.dial PTV @19:40:33.05 delay 5 seconds @19:40:38.07 PSV.dial :air @19:40:38.84 PS.seek 0ml @19:41:02.93 Additional air clear @19:41:02.94 PSR.select :air @19:41:02.95 PSV.dial PSR @19:41:04.32 Pulling 2ml of PSR.air @19:41:04.33 PSR.select :air @19:41:04.35 PS.reconfigure :default @19:41:04.40 PS.seek 2ml @19:41:25.64 delay 5 seconds @19:41:30.66 PTV.dial :puck @19:41:30.68 PSV.dial PTV @19:41:31.42 PBV.dial :waste1 @19:41:31.86 PS.seek 1.5ml @19:41:37.77 delay 3 seconds @19:41:40.78 PBV.dial between PSV and waste1 @19:41:41.16 delay 1 second @19:41:42.18 PSV.dial :waste1 @19:41:43.24 PS.seek 0ml @19:41:59.63 Processing Puck Heater at 28C before imaging @19:41:59.64 PH.coast @19:41:59.76 Imaging da puck in Processing Clamp while storing it to tube 1 @19:41:59.92 SC.reconfigure :tight @19:41:59.97 SC.seek 1 @19:42:03.52 SC.configure :limp @19:42:03.71 Elbow.reconfigure :tight @19:42:03.75 Elbow.seek 1 @19:42:05.49 Forearm.reconfigure :tight @19:42:05.54 Forearm.seek 1 + 450 counts @19:42:06.64 SE.reconfigure :pick @19:42:06.70 SE.seek :beamBreak @19:42:09.69 SE.configure :default @19:42:09.83 Elbow.reconfigure :limp @19:42:09.88 Forearm.reconfigure :loose @19:42:09.92 Forearm.seek 1 @19:42:10.18 Elbow.hold @19:42:10.24 Elbow.reconfigure :tight @19:42:10.33 SE.seek 1.19 Pucks below Top Plate @19:42:10.81 Forearm.force -20 @19:42:10.85 Forearm.seek 1 + 308 counts @19:42:11.15 Forearm.reconfigure :tight @19:42:11.20 Forearm.seek 1 + 450 counts @19:42:11.42 SE.seek 2.19 Pucks below Top Plate @19:42:11.92 Forearm.reconfigure :loose @19:42:11.96 Forearm.seek :retract @19:42:12.89 Elbow.seek PC @19:42:15.58 Elbow.reconfigure :loose @19:42:15.67 Forearm.seek PC @19:42:16.63 Hand.close @19:42:17.27 PC.seek :open @19:42:22.02 Forearm.seek :retract @19:42:22.95 Elbow.reconfigure :tight @19:42:22.99 Elbow.seek 1 @19:42:25.54 Forearm.reconfigure :tight @19:42:25.58 Forearm.seek 1 + 450 counts @19:42:26.54 Hand.open @19:42:27.21 SE.seek 1.84 Pucks below Top Plate @19:42:27.49 SE.reconfigure :seal @19:42:27.53 SE.seek 0.87 Pucks below Top Plate @19:42:28.00 Lights, camera, ACTION! @19:42:28.04 PuckCamera: snap -bin=2,2 -auto /var/log/esp/da22oct1319h100ml.tif @19:42:47.58 Waiting >20 seconds for da to relinquish FlushPuck @19:42:48.26 SX HX9/16 (USB:02): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 16.692 seconds /var/log/esp/da22oct1319h100ml.tif: TIFF Upload Complete @19:42:48.29 PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da22oct1319h100ml15s.tif @19:43:03.91 SX HX9/16 (USB:02): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15 seconds /var/log/esp/midres/da22oct1319h100ml15s.tif: TIFF Upload Complete @19:43:03.93 PuckCamera: snap -bin=2,2 20.0 /var/log/esp/midres/da22oct1319h100ml20s.tif @19:43:24.58 SX HX9/16 (USB:02): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 20 seconds /var/log/esp/midres/da22oct1319h100ml20s.tif: TIFF Upload Complete @19:43:24.60 Gate.power :camera,:OFF @19:43:24.62 DAfans.email "16.692 sec auto-exposure",Subject: "da22oct1319h100ml images!" @19:43:24.86 Elbow.seek 1 @19:43:25.07 Forearm.seek 1 + 450 counts @19:43:25.15 SE.reconfigure :gently @19:43:25.19 SE.seek 1.59 Pucks below Top Plate @19:43:25.83 Elbow.reconfigure :limp @19:43:25.90 Forearm.reconfigure :loose @19:43:26.53 Forearm.seek 1 @19:43:26.79 Elbow.hold @19:43:26.86 Elbow.reconfigure :tight @19:43:26.93 SE.reconfigure :default @19:43:26.97 SE.seek 1.77 Pucks below Top Plate @19:43:27.37 Forearm.force -20 @19:43:27.45 Forearm.seek 1 + 286 counts @19:43:27.77 Forearm.reconfigure :tight @19:43:27.81 Forearm.seek 1 + 450 counts @19:43:28.07 SE.seek :down @19:43:29.21 Forearm.reconfigure :loose @19:43:29.27 Forearm.seek :retract @19:43:30.67 SE.stop @19:43:30.88 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @19:43:31.46 Elbow.seek FlushPuck::Garage @19:43:33.52 Elbow.reconfigure :loose @19:43:33.71 Forearm.seek :garage @19:43:35.27 Hand.close @19:43:36.15 Forearm.seek :retract @19:43:37.18 Elbow.reconfigure :tight @19:43:37.27 Elbow.seek PC @19:43:38.80 Elbow.reconfigure :loose @19:43:39.00 Forearm.reconfigure :tight @19:43:39.09 Forearm.seek PC @19:43:40.69 PC.seek :closed @19:43:45.49 Processing Clamp onSpring/offSpring for 16/26 samples, on/off torque 1.62 @19:43:45.69 Hand.open @19:43:46.44 Forearm.reconfigure :loose @19:43:46.52 Forearm.seek :retract @19:43:47.57 PSR.select :air @19:43:47.63 Storing wcr puck in Collection Clamp to tube 1 @19:43:47.67 PSV.dial PSR @19:43:48.10 SC.reconfigure :tight @19:43:48.19 SC.seek 1 @19:43:48.36 SC.configure :limp @19:43:48.68 Pulling 0.5ml of PSR.flush @19:43:48.74 PSR.select :flush @19:43:49.00 Elbow.reconfigure :tight @19:43:49.09 Elbow.seek 1 @19:43:49.21 PS.seek 0.5ml @19:43:51.79 Forearm.reconfigure :tight @19:43:51.89 Forearm.seek 1 + 450 counts @19:43:53.04 SE.reconfigure :pick @19:43:53.15 SE.seek :beamBreak @19:43:55.36 delay 5 seconds @19:43:56.21 SE.configure :default @19:43:56.51 Elbow.reconfigure :limp @19:43:56.62 Forearm.reconfigure :loose @19:43:56.70 Forearm.seek 1 @19:43:57.02 Elbow.hold @19:43:57.16 Elbow.reconfigure :tight @19:43:57.34 SE.seek 2.17 Pucks below Top Plate @19:43:58.00 Forearm.force -20 @19:43:58.08 Forearm.seek 1 + 298 counts @19:43:59.58 Forearm.reconfigure :tight @19:43:59.72 Forearm.seek 1 + 450 counts @19:43:59.96 SE.seek 3.17 Pucks below Top Plate @19:44:00.38 upsync @19:44:00.49 PSR.select :air @19:44:00.56 Forearm.reconfigure :loose @19:44:00.61 Forearm.seek :retract @19:44:00.66 PS.seek 2.5ml @19:44:01.54 Elbow.seek CC @19:44:03.14 Elbow.reconfigure :loose @19:44:03.24 Forearm.seek CC @19:44:04.19 Hand.close @19:44:04.77 CC.seek :open @19:44:09.48 Forearm.seek :retract @19:44:10.43 Elbow.reconfigure :tight @19:44:10.47 Elbow.seek 1 @19:44:12.24 Elbow.reconfigure :limp @19:44:12.29 Forearm.seek 1 @19:44:13.27 Elbow.hold @19:44:13.34 Elbow.reconfigure :tight @19:44:13.39 Hand.open @19:44:14.14 Forearm.force -20 @19:44:14.17 Forearm.seek 1 + 318 counts @19:44:14.46 Forearm.reconfigure :tight @19:44:14.50 Forearm.seek 1 + 450 counts @19:44:14.76 SE.seek :down @19:44:15.89 Forearm.reconfigure :loose @19:44:15.94 Forearm.seek :retract @19:44:17.25 SE.stop @19:44:17.28
Starting finalPurge @19:44:21.95 delay 1 second @19:44:22.97 PRV.dial :waste2,avoiding: :ab2 @19:44:24.81 PTV.dial :PRVmixing @19:44:26.11 PSV.dial :mixloop @19:44:27.13 PS.seek :empty @19:44:37.18
Waiting >20 seconds for psFlush to relinquish FlushPuck @19:44:53.52 PSR.select :air @19:44:53.53 PSV.dial PSR @19:44:54.61 Pulling 0.5ml of PSR.flush @19:44:54.62 PSR.select :flush @19:44:54.74 PS.seek 0.5ml @19:45:00.64 delay 5 seconds @19:45:05.66 PSR.select :air @19:45:05.77 PS.seek 2.5ml @19:45:27.04 delay 1 second @19:45:28.06 PBV.dial :waste1 @19:45:28.43 PTV.dial :mixing @19:45:29.18 PSV.dial :mixloop @19:45:30.23 PS.seek :empty @19:45:56.57 PSV.dial PSR @19:45:57.60 PSR.select :flush @19:45:57.72 PS.seek 1ml @19:46:08.95 PSR.select :air @19:46:09.06 PS.seek 2.5ml @19:46:25.26 PSV.dial PTV @19:46:26.01 PTV.dial :puck @19:46:26.75 PBV.dial :waste1 @19:46:26.77 PS.seek 0ml @19:46:53.04 PSV.dial PSR @19:46:53.79 PSR.select :flush @19:46:53.91 PS.seek 0.5ml @19:46:59.88 PSR.select :air @19:47:00.00 PS.seek 2.5ml @19:47:21.26 PSV.dial PTV @19:47:22.01 PBV.dial PSV @19:47:22.58 PS.seek 0ml @19:47:49.13 PSV.dial PBV @19:47:49.61 PS.seek 2.5ml @19:48:15.94 PSV.dial :waste1 @19:48:16.69 PS.seek 0ml @19:48:43.10 PSV.dial PSR @19:48:43.57 PSR.select :air @19:48:43.59 PS.seek 2.5ml @19:49:09.91 PSV.dial PTV @19:49:11.10 PBV.dial PSV @19:49:11.12 PS.seek 0ml @19:49:37.44 PSV.dial PBV @19:49:37.92 PS.seek 2.5ml @19:50:04.29 PSV.dial :waste1 @19:50:05.03 PS.seek 0ml @19:50:31.47 PSV.dial PSR @19:50:32.05 PSR.select :flush @19:50:32.17 PS.seek 1ml @19:50:43.25 PSR.select :air @19:50:43.37 PS.seek 2.5ml @19:50:59.57 PSV.dial PTV @19:51:00.32 PTV.dial :puck @19:51:00.33 PBV.dial :waste1 @19:51:00.89 PS.seek 0ml @19:51:27.28 PSV.dial PSR @19:51:28.04 PSR.select :flush @19:51:28.15 PS.seek 0.5ml @19:51:34.10 PSR.select :air @19:51:34.21 PS.seek 2.5ml @19:51:55.44 PSV.dial PTV @19:51:56.19 PBV.dial PSV @19:51:56.75 PS.seek 0ml @19:52:23.13 PSV.dial PBV @19:52:23.60 PS.seek 2.5ml @19:52:49.97 PSV.dial :waste1 @19:52:50.71 PS.seek 0ml @19:53:17.22 PSV.dial PSR @19:53:17.69 PSR.select :air @19:53:17.71 PS.seek 2.5ml @19:53:44.13 PSV.dial PTV @19:53:44.86 PBV.dial PSV @19:53:44.88 PS.seek 0ml @19:54:11.25 PSV.dial PBV @19:54:11.73 PS.seek 2.5ml @19:54:38.06 PSV.dial :waste1 @19:54:38.80 PS.seek 0ml @19:55:05.19
Loading FlushPuck into Collection Clamp from Processing Clamp @19:55:05.37 Elbow.seek PC @19:55:07.86 Elbow.reconfigure :loose @19:55:07.95 Forearm.seek PC @19:55:08.88 Hand.close @19:55:09.51 PC.seek :open @19:55:14.21 Forearm.seek :retract @19:55:15.19 Elbow.reconfigure :tight @19:55:15.23 Elbow.seek CC @19:55:18.83 Elbow.reconfigure :loose @19:55:18.92 Forearm.reconfigure :tight @19:55:18.96 Forearm.seek CC @19:55:20.25 CC.seek :closed @19:55:24.75 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.29 @19:55:24.81 Hand.open @19:55:25.45 Forearm.reconfigure :loose @19:55:25.49 Forearm.seek :retract @19:55:26.47 Flushing the Collection Valve::Manifold @19:55:26.58 CSV.dial CSR @19:55:27.18 Pulling 5ml of CSR.flush @19:55:27.19 CSR.select :flush @19:55:27.30 CS.seek 5ml @19:55:41.10 delay 5 seconds @19:55:46.12 CSR.select :air @19:55:46.27 CS.reconfigure :fast @19:55:46.31 CS.seek 7.501ml @19:55:53.06 CSV.dial :waste @19:55:54.32 CS.reconfigure :default @19:55:54.36 CS.seek 0ml @19:56:14.44 Flushing lower manifold's waste1 port @19:56:14.56 CSV.dial :flush @19:56:15.01 CS.reconfigure :fast @19:56:15.05 CS.seek 5ml @19:56:27.69 delay 5 seconds @19:56:32.70 CSV.dial :air @19:56:33.20 CS.seek 7ml @19:56:38.75 CSV.dial CTV @19:56:38.79
CTV.dial CSV @19:56:40.61 CBV.dial :waste1 @19:56:41.37 CS.seek 0ml @19:56:57.94 Flushing lower manifold's waste2 port @19:56:58.06 CSV.dial :flush @19:56:58.79 CS.seek 5ml @19:57:11.56 delay 5 seconds @19:57:16.58 CSV.dial :air @19:57:17.10 CS.seek 7.001ml @19:57:22.60 CSV.dial CTV @19:57:22.62
CTV.dial CSV @19:57:23.07 CBV.dial :waste2 @19:57:24.09 CS.seek 0ml @19:57:40.69 Flushing lower manifold's waste2b port @19:57:40.81 CSV.dial :flush @19:57:41.54 CS.seek 5ml @19:57:54.13 delay 5 seconds @19:57:59.14 CSV.dial :air @19:57:59.66 CS.seek 7ml @19:58:05.13 CSV.dial CTV @19:58:05.15
CTV.dial CSV @19:58:05.60 CBV.dial :waste2b @19:58:06.63 CS.seek 0ml @19:58:23.16 Disinfecting external Intake with kill @19:58:23.27 CSV.dial CSR @19:58:23.76 Pulling 8ml of CSR.kill @19:58:23.77 CSR.select :kill @19:58:23.88 CS.seek 8ml @19:58:43.60 delay 5 seconds @19:58:48.61 CSR.select :air @19:58:48.73 CS.seek 10ml @19:58:54.19 CSV.dial CBV @19:58:54.21
CBV.dial CSV @19:58:55.25 CTV.dial :intake @19:58:56.03 Intake.select :open @19:58:56.14 CS.seek 2ml @19:59:14.94 Intake.select :close @19:59:15.03 CTV.dial :air @19:59:15.80 CS.reconfigure :default @19:59:15.84 CS.seek 0ml @19:59:21.78 CSV.dial CSR @19:59:22.85 Pulling 5ml of CSR.flush @19:59:22.86 CSR.select :flush @19:59:22.97 CS.seek 5ml @19:59:36.72 delay 5 seconds @19:59:41.74 CSR.select :air @19:59:41.85 CS.seek 10ml @19:59:55.40 CSV.dial CBV @19:59:56.43 CS.seek 0ml @20:00:22.81 Garaging FlushPuck in Collection Clamp @20:00:22.88 Elbow.reconfigure :tight @20:00:22.92 Elbow.seek CC @20:00:23.19 Elbow.reconfigure :loose @20:00:23.27 Forearm.seek CC @20:00:24.27 Hand.close @20:00:24.89 CC.seek :open @20:00:29.51 Forearm.seek :retract @20:00:30.49 Elbow.reconfigure :tight @20:00:30.54 Elbow.seek :garage @20:00:33.44 Elbow.reconfigure :loose @20:00:33.53 Forearm.reconfigure :tight @20:00:33.57 Forearm.seek :garage @20:00:34.54 Hand.open @20:00:35.19 Forearm.reconfigure :loose @20:00:35.23 Forearm.seek :retract @20:00:36.18 Completed [initialPurge; da [100, 100] #da and wsr are run simultanelously] begun 18:08:28 @20:00:36.23 Asleep after completing DA_WCR_101322 assays @20:00:36.24 GoodNews.email "{1 => 4,\n 2 => 29,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@19:57:48, 17% flow, 22.0C, 20% humidity, 15.0psia, 13.749V, 0.435A, 0.423A avg, 5.99W\nSampled 100.0ml",Subject: "Asleep after completing DA_WCR_101322 assays" @20:00:40.73 upsync No 'DA_WCR_11822.rb' script found on ESPpath: /home/esp/esp2/mission:. No 'DA_WCR_11922.rb' script found on ESPpath: /home/esp/esp2/mission:. @16:49:33.80UTC09-Nov-22
ESP2 v3.41 I2C Gateway v3.20 for core bus @16:49:36.34 Configuration for short puck jake 1/11/21 by brent@mbari.org /home/esp/esp2/type/shallow/jake/configure.rb @16:49:40.27 Polling Can every 10:00 @16:49:40.31 Cleanly exited at 16:27:40UTC09-Nov-22 @16:49:40.34 Loading /home/esp/esp2/mission/DA_WCR_110822.rb mission @16:49:40.40 Preparing to sleep until + 00:00:05 @16:49:40.41 GoodNews.email "Starting DA_WCR_110822 w/pucks in tube 2\n{1 => 4,\n 2 => 29,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@16:49:40, 20% flow, 13.2C, 24% humidity, 14.5psia, 13.773V, 0.290A, 0.278A avg, 4.00W",Subject: "DA_WCR_110822 begun" @16:49:40.45
Asleep til + 00:00:05 @16:49:40.49 Continuing at 16:49 @16:49:40.73 Gate.power :core,:OFF @16:49:40.75 Gate.power :raw,:OFF Shutdown @16:49:44.43 GoodNews.email "Then, in tube 2: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2/mission/DA_WCR_110822.rb:14\n{1 => 4,\n 2 => 29,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@16:49:40, 20% flow, 13.2C, 24% humidity, 14.5psia, 13.773V, 0.290A, 0.278A avg, 4.00W",Subject: "Asleep until 16:49" @16:49:45.38
Powering core bus @16:49:47.02 upsync @16:49:49.29 upsync @16:49:50.65
Gate.power :raw,:ON @16:49:51.49 CS.configure :default @16:49:51.58 PS.configure :default @16:49:51.67 SS.configure :default @16:49:51.76 CC.configure :default @16:49:51.85 PC.configure :default @16:49:51.96 Forearm.configure :loose @16:49:52.04 Elbow.configure :loose @16:49:52.15 SC.configure :loose @16:49:52.23 SE.configure :default @16:49:52.27 CH.coast @16:49:52.32 CH.configure :default @16:49:52.35 PH.coast @16:49:52.40 PH.configure :default @16:49:52.44 All dwarf controllers running firmware version 3.85 @16:49:52.54 Valve.reset @16:49:52.72 Intake.select :close @16:49:52.77 Exhaust.select :close @16:49:52.81 CTV.dial :air @16:49:52.94 PSV.dial :air @16:49:52.97 CSV.dial :waste1 @16:49:53.10
Forearm.reconfigure :loose @16:49:53.11 PS.reconfigure :default @16:49:53.21
Forearm.home.seek :retract @16:49:53.22 PS.home.seek 0ml @16:49:53.45 SSV.dial :spit,avoiding: :exhaust @16:49:53.58 CS.reconfigure :fast @16:49:53.61 SS.reconfigure :default @16:49:53.70 CS.home.seek 0ml @16:49:53.72 SS.home.seek 0ml @16:49:53.97 Hand.close @16:49:54.01
Elbow.jog 1500 @16:49:54.55 Elbow.reconfigure :loose @16:49:54.62 Elbow.home.seek :clear @16:49:57.95 SE.reconfigure :default @16:49:57.99 SE.home.seek :down @16:49:58.60 SC.reconfigure :tight @16:49:58.65 SC.home.seek :initial @16:50:01.47 PS.stop @16:50:27.00
SC.configure :limp @16:50:27.05 Forearm.seek :retract @16:50:27.11 Elbow.seek :hammer @16:50:28.14 Forearm.reconfigure :tight @16:50:28.18 Forearm.seek retracted @16:50:28.60 PC.reconfigure :default @16:50:28.62
CC.reconfigure :default @16:50:28.71 CC.home.seek :open @16:50:28.72 PC.home.seek :open @16:50:32.24
Phase [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] @16:50:32.26 Notice.email "At 16:50:32 began in tube 2: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2/mission/DA_WCR_110822.rb:14\n{1 => 4,\n 2 => 29,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@16:50:32, 21% flow, 13.2C, 23% humidity, 14.5psia, 13.764V, 0.315A, 0.302A avg, 4.33W",Subject: "Started DA_WCR_110822 phase initialPurge; da [1000, 1000] #da and wsr are run simultanelously" @16:50:32.28
Starting initialPurge @16:50:32.30 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @16:50:32.37 Hand.open @16:50:33.09 Elbow.reconfigure :tight @16:50:33.13 Elbow.seek FlushPuck::Garage @16:50:35.10 Elbow.reconfigure :loose @16:50:35.19 Forearm.reconfigure :loose @16:50:35.23 Forearm.seek :garage @16:50:36.18 Hand.close @16:50:36.96 Forearm.seek :retract @16:50:37.28 upsync @16:50:37.97
Elbow.reconfigure :tight @16:50:38.01 Elbow.seek CC @16:50:41.05 Elbow.reconfigure :loose @16:50:41.14 Forearm.reconfigure :tight @16:50:41.19 Forearm.seek CC @16:50:42.48 CC.seek :closed @16:50:47.07 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.32 @16:50:47.15 Hand.open @16:50:47.76 Forearm.reconfigure :loose @16:50:47.80 Forearm.seek :retract @16:50:48.82 Flushing the Collection Valve::Manifold @16:50:48.93 CSV.dial CSR @16:50:50.39 Pulling 5ml of CSR.flush @16:50:50.40 CSR.select :flush @16:50:50.51 CS.reconfigure :default @16:50:50.55 CS.seek 5ml @16:51:04.15 delay 5 seconds @16:51:09.17 CSR.select :air @16:51:09.31 CS.reconfigure :fast @16:51:09.36 CS.seek 7.5ml @16:51:16.05 CSV.dial :waste @16:51:17.32 CS.reconfigure :default @16:51:17.37 CS.seek 0ml @16:51:37.40 Purging external Intake @16:51:37.52 CSV.dial :flush @16:51:37.97 CS.reconfigure :fast @16:51:38.01 CS.seek 10ml @16:52:02.55 delay 5 seconds @16:52:07.57 CSV.dial CBV @16:52:07.60
CBV.dial CSV @16:52:08.37 CTV.dial :intake @16:52:09.15 Intake.select :open @16:52:09.25 CS.seek 0ml @16:52:32.58 Intake.select :close @16:52:32.68 CTV.dial :air @16:52:33.48 Flushing upper manifold @16:52:33.60 CSV.dial :flush @16:52:34.33 CS.seek 5ml @16:52:47.12 delay 5 seconds @16:52:52.13 CSV.dial :air @16:52:52.65 CS.seek 7ml @16:52:58.21 CSV.dial CBV @16:52:58.23
CBV.dial CSV @16:52:59.27 CTV.dial :air @16:52:59.29 CS.seek 0ml @16:53:16.15 delay 7 seconds @16:53:23.17 CS.seek 3ml @16:53:31.14 CSV.dial :waste @16:53:31.59 CS.seek 0ml @16:53:39.41 CSV.dial :flush @16:53:39.84 CS.seek 3ml @16:53:47.96 delay 5 seconds @16:53:52.98 CSV.dial :air @16:53:53.49 CS.seek 5ml @16:53:59.14 CSV.dial :air @16:53:59.16 CS.seek 0ml @16:54:11.49 delay 7 seconds @16:54:18.52 CS.seek 3ml @16:54:26.49 CSV.dial :waste @16:54:27.23 CS.seek 0ml @16:54:35.12 Garaging FlushPuck in Collection Clamp @16:54:35.18 Elbow.reconfigure :tight @16:54:35.23 Elbow.seek CC @16:54:35.67 Elbow.reconfigure :loose @16:54:35.76 Forearm.seek CC @16:54:36.70 Hand.close @16:54:37.33 CC.seek :open @16:54:41.97 Forearm.seek :retract @16:54:43.01 Elbow.reconfigure :tight @16:54:43.05 Elbow.seek :garage @16:54:46.10 Elbow.reconfigure :loose @16:54:46.18 Forearm.reconfigure :tight @16:54:46.23 Forearm.seek :garage @16:54:47.13 Hand.open @16:54:47.83 Forearm.reconfigure :loose @16:54:47.87 Forearm.seek :retract @16:54:48.83 da sampling at most 1000ml, wcr at most 1000ml @16:54:48.86 Loading sh1 puck from tube 2 into Collection Clamp @16:54:48.87 DAfans.email "Started da in tube 2 at 16:54:48UTC09-Nov-22\n{1 => 4,\n 2 => 29,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@16:50:32, 21% flow, 13.2C, 23% humidity, 14.5psia, 13.764V, 0.315A, 0.302A avg, 4.33W",Subject: "da sampling at most 1000ml, wcr at most 1000ml" @16:54:49.12
SC.reconfigure :tight @16:54:49.17 SC.seek 2 @16:54:54.26 upsync @16:54:55.85
SC.configure :limp @16:54:56.06 Elbow.reconfigure :tight @16:54:56.10 Elbow.seek 2 @16:54:58.20 Forearm.reconfigure :tight @16:54:58.25 Forearm.seek 2 + 450 counts @16:54:59.31 SE.reconfigure :pick @16:54:59.36 SE.seek :beamBreak @16:55:00.73 SE.configure :default @16:55:00.87 Elbow.seek 2 @16:55:01.42 Forearm.seek 2 + 300 counts @16:55:01.87 Forearm.reconfigure :loose @16:55:01.91 Hand.close @16:55:02.59 SE.seek :down @16:55:02.78
Forearm.seek :retract @16:55:03.45 SE.stop @16:55:03.75
Elbow.seek CC @16:55:05.43 Elbow.reconfigure :loose @16:55:05.52 Forearm.reconfigure :tight @16:55:05.56 Forearm.seek CC @16:55:06.82 CC.seek :closed @16:55:11.37 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.14 @16:55:11.38 Hand.open @16:55:12.01 Forearm.reconfigure :loose @16:55:12.05 Forearm.seek :retract @16:55:13.03 collectSample 1000ml w/bubble point of 24psi @16:55:13.04 Priming 7ml @16:55:13.05 Intake.select :close @16:55:13.06 Exhaust.select :close @16:55:13.07 SS.hold @16:55:13.26 Calibrating pressure and temperature sensors @16:55:13.29 CTV.dial :air @16:55:13.30 SSV.dial :spit,avoiding: :exhaust @16:55:13.32 SS.reconfigure :fast @16:55:13.36 SS.seek 5ml @16:55:16.10 SSV.dial CBV @16:55:16.12
CBV.dial SSV @16:55:17.47 delay 3.8 seconds @16:55:21.39 SSV.dial :spit @16:55:22.70 SS.seek 0ml @16:55:25.62 SSV.dial CBV @16:55:26.92 CTV.dial :intake @16:55:27.71 SSV.dial CBV @16:55:27.73
CBV.dial SSV @16:55:27.75 Allowing 40s for 7.0ml of remaining 7.0ml @16:55:27.76 Intake.select :open @16:55:29.91 Intake @15.6psia, vacuum from 10.0 to 13.0psi @16:55:29.93 SS.reconfigure :inhaling 2.20ml/s @16:55:29.97 SS.seek 7ml @16:55:35.47 Stroke completed -- waiting for pressure to equalize ... @16:55:35.48 SS.reconfigure :exhale @16:55:35.52 SS.hold @16:55:38.41 Intake.select :close @16:55:38.55 SSV.dial :exhaust @16:55:39.30 Exhaust.select :open @16:55:39.40 SS.seek 0ml @16:55:43.19 Exhaust.select :close @16:55:43.30 Sampling 1000.0ml Intake.select :close @16:55:43.31 Exhaust.select :close @16:55:43.32 SS.reconfigure :default @16:55:43.36 SS.hold @16:55:43.54 CTV.dial :intake @16:55:43.56 SSV.dial CBV @16:55:43.59
CBV.dial SSV @16:55:44.33 Allowing 130s for 25.0ml of remaining 1000.0ml @16:55:44.34 Intake.select :open @16:55:46.49 Intake @15.8psia, vacuum from 10.0 to 13.0psi @16:55:46.51 SS.reconfigure :inhaling 2.20ml/s @16:55:46.55 SS.seek 25ml @16:55:55.40 Vacuum too high -- waiting up to 40 seconds @16:55:55.41 SS.reconfigure :exhale @16:55:55.45 SS.hold @16:55:56.49 Intake @11.6psia, vacuum from 6.1 to 9.1psi @16:55:56.58 SS.reconfigure :inhaling 1.98ml/s @16:55:56.63 SS.seek 25ml @16:56:03.43 Vacuum too high -- waiting up to 40 seconds @16:56:03.44 SS.reconfigure :exhale @16:56:03.48 SS.hold @16:56:09.34 Intake @13.1psia, vacuum from 7.6 to 10.6psi @16:56:09.35 SS.reconfigure :inhaling 1.79ml/s @16:56:09.40 SS.seek 25ml @16:56:10.20 Stroke completed -- waiting for pressure to equalize ... @16:56:10.21 SS.reconfigure :exhale @16:56:10.25 SS.hold @16:56:10.64 Intake.select :close @16:56:10.78 SSV.dial :exhaust @16:56:11.53 Exhaust.select :open @16:56:11.64 SS.seek 0ml @16:56:20.56 Exhaust.select :close @16:56:20.67 SSV.dial CBV @16:56:20.68
CBV.dial SSV @16:56:21.45 Allowing 130s for 25.0ml of remaining 975.0ml @16:56:21.46 Intake.select :open @16:56:23.62 Intake @15.3psia, vacuum from 9.8 to 12.8psi @16:56:23.64 SS.reconfigure :inhaling 1.79ml/s @16:56:23.68 SS.seek 25ml @16:56:36.61 Vacuum too high -- waiting up to 40 seconds @16:56:36.62 SS.reconfigure :exhale @16:56:36.67 SS.hold @16:56:37.24 Intake @11.5psia, vacuum from 6.0 to 9.0psi @16:56:37.25 SS.reconfigure :inhaling 1.61ml/s @16:56:37.30 SS.seek 25ml @16:56:41.89 Stroke completed -- waiting for pressure to equalize ... @16:56:41.90 SS.reconfigure :exhale @16:56:41.94 SS.hold @16:56:48.44 Intake.select :close @16:56:48.58 SSV.dial :exhaust @16:56:49.34 Exhaust.select :open @16:56:49.45 SS.seek 0ml @16:56:57.96 Exhaust.select :close @16:56:58.07 SSV.dial CBV @16:56:58.09
CBV.dial SSV @16:56:58.86 Allowing 130s for 25.0ml of remaining 950.0ml @16:56:58.87 Intake.select :open @16:57:01.02 Intake @15.1psia, vacuum from 9.6 to 12.6psi @16:57:01.04 SS.reconfigure :inhaling 1.61ml/s @16:57:01.08 SS.seek 25ml @16:57:18.68 Vacuum too high -- waiting up to 40 seconds @16:57:18.69 SS.reconfigure :exhale @16:57:18.73 SS.hold @16:57:19.26 Intake @11.5psia, vacuum from 6.0 to 9.0psi @16:57:19.27 SS.reconfigure :inhaling 1.45ml/s @16:57:19.32 SS.seek 25ml @16:57:21.07 Stroke completed -- waiting for pressure to equalize ... @16:57:21.08 SS.reconfigure :exhale @16:57:21.12 SS.hold @16:57:27.60 Intake.select :close @16:57:27.74 SSV.dial :exhaust @16:57:28.50 Exhaust.select :open @16:57:28.61 SS.seek 0ml @16:57:37.15 Exhaust.select :close @16:57:37.25 SSV.dial CBV @16:57:37.28
CBV.dial SSV @16:57:38.05 Allowing 130s for 25.0ml of remaining 925.0ml @16:57:38.06 Intake.select :open @16:57:40.21 Intake @15.1psia, vacuum from 9.6 to 12.6psi @16:57:40.22 SS.reconfigure :inhaling 1.45ml/s @16:57:40.27 SS.seek 25ml @16:58:00.78 Stroke completed -- waiting for pressure to equalize ... SS.reconfigure :exhale @16:58:00.82 SS.hold @16:58:08.20 Intake.select :close @16:58:08.33 SSV.dial :exhaust @16:58:09.09 Exhaust.select :open @16:58:09.20 SS.seek 0ml @16:58:18.15 Exhaust.select :close @16:58:18.25 SSV.dial CBV @16:58:18.31
CBV.dial SSV @16:58:19.01 Allowing 130s for 25.0ml of remaining 900.0ml @16:58:19.02 Intake.select :open @16:58:21.17 Intake @15.2psia, vacuum from 9.7 to 12.7psi @16:58:21.19 SS.reconfigure :inhaling 1.45ml/s @16:58:21.23 SS.seek 25ml @16:58:41.18 Stroke completed -- waiting for pressure to equalize ... @16:58:41.19 SS.reconfigure :exhale @16:58:41.23 SS.hold @16:58:49.08 Intake.select :close @16:58:49.22 SSV.dial :exhaust @16:58:49.98 Exhaust.select :open @16:58:50.09 SS.seek 0ml @16:58:58.81 Exhaust.select :close @16:58:58.91 SSV.dial CBV @16:58:58.93
CBV.dial SSV @16:58:59.70 Allowing 130s for 25.0ml of remaining 875.0ml @16:58:59.71 Intake.select :open @16:59:01.86 Intake @15.2psia, vacuum from 9.7 to 12.7psi @16:59:01.88 SS.reconfigure :inhaling 1.45ml/s @16:59:01.92 SS.seek 25ml @16:59:21.39 Vacuum too high -- waiting up to 40 seconds @16:59:21.40 SS.reconfigure :exhale @16:59:21.44 SS.hold @16:59:21.96 Intake @12.0psia, vacuum from 6.5 to 9.5psi @16:59:21.97 SS.reconfigure :inhaling 1.30ml/s @16:59:22.02 SS.seek 25ml @16:59:23.79 Stroke completed -- waiting for pressure to equalize ... @16:59:23.80 SS.reconfigure :exhale @16:59:23.84 SS.hold @16:59:31.20 Intake.select :close @16:59:31.34 SSV.dial :exhaust @16:59:32.11 Exhaust.select :open @16:59:32.22 SS.seek 0ml @16:59:40.79 Exhaust.select :close @16:59:40.89 SSV.dial CBV @16:59:40.91
CBV.dial SSV @16:59:41.68 Allowing 130s for 25.0ml of remaining 850.0ml @16:59:41.69 Intake.select :open @16:59:43.84 Intake @15.2psia, vacuum from 9.7 to 12.7psi @16:59:43.86 SS.reconfigure :inhaling 1.30ml/s @16:59:43.90 SS.seek 25ml @17:00:06.07 Stroke completed -- waiting for pressure to equalize ... @17:00:06.08 SS.reconfigure :exhale @17:00:06.12 SS.hold @17:00:13.47 Intake.select :close @17:00:13.60 SSV.dial :exhaust @17:00:14.35 Exhaust.select :open @17:00:14.46 SS.seek 0ml @17:00:23.40 Exhaust.select :close @17:00:23.50 SSV.dial CBV @17:00:23.53
CBV.dial SSV @17:00:24.29 Allowing 130s for 25.0ml of remaining 825.0ml @17:00:24.30 Intake.select :open @17:00:26.45 Intake @15.2psia, vacuum from 9.7 to 12.7psi @17:00:26.47 SS.reconfigure :inhaling 1.30ml/s @17:00:26.51 SS.seek 25ml @17:00:47.55 Vacuum too high -- waiting up to 40 seconds @17:00:47.56 SS.reconfigure :exhale @17:00:47.60 SS.hold @17:00:48.32 Intake @12.2psia, vacuum from 6.7 to 9.7psi @17:00:48.33 SS.reconfigure :inhaling 1.17ml/s @17:00:48.37 SS.seek 25ml @17:00:49.41 Stroke completed -- waiting for pressure to equalize ... @17:00:49.42 SS.reconfigure :exhale @17:00:49.46 SS.hold @17:00:56.84 Intake.select :close @17:00:56.97 SSV.dial :exhaust @17:00:57.73 Exhaust.select :open @17:00:57.83 SS.seek 0ml @17:01:06.54 Exhaust.select :close @17:01:06.64 SSV.dial CBV @17:01:06.66
CBV.dial SSV @17:01:07.43 Allowing 130s for 25.0ml of remaining 800.0ml @17:01:07.44 Intake.select :open @17:01:09.59 Intake @15.2psia, vacuum from 9.7 to 12.7psi @17:01:09.61 SS.reconfigure :inhaling 1.17ml/s @17:01:09.65 SS.seek 25ml @17:01:33.83 Stroke completed -- waiting for pressure to equalize ... @17:01:33.84 SS.reconfigure :exhale @17:01:33.88 SS.hold @17:01:41.25 Intake.select :close @17:01:41.38 SSV.dial :exhaust @17:01:42.62 Exhaust.select :open @17:01:42.72 SS.seek 0ml @17:01:51.41 Exhaust.select :close @17:01:51.52 SSV.dial CBV @17:01:51.57
CBV.dial SSV @17:01:52.29 Allowing 130s for 25.0ml of remaining 775.0ml @17:01:52.30 Intake.select :open @17:01:54.45 Intake @15.2psia, vacuum from 9.7 to 12.7psi @17:01:54.47 SS.reconfigure :inhaling 1.17ml/s @17:01:54.51 SS.seek 25ml @17:02:18.77 Stroke completed -- waiting for pressure to equalize ... @17:02:18.78 SS.reconfigure :exhale @17:02:18.82 SS.hold @17:02:27.29 Intake.select :close @17:02:27.42 SSV.dial :exhaust @17:02:28.18 Exhaust.select :open @17:02:28.29 SS.seek 0ml @17:02:36.97 Exhaust.select :close @17:02:37.08 SSV.dial CBV @17:02:37.11
CBV.dial SSV @17:02:37.85 Allowing 130s for 25.0ml of remaining 750.0ml @17:02:37.86 Intake.select :open @17:02:40.02 Intake @15.3psia, vacuum from 9.8 to 12.8psi @17:02:40.03 SS.reconfigure :inhaling 1.17ml/s @17:02:40.08 SS.seek 25ml @17:03:03.57 Vacuum too high -- waiting up to 40 seconds @17:03:03.58 SS.reconfigure :exhale @17:03:03.63 SS.hold @17:03:05.68 Intake @13.0psia, vacuum from 7.5 to 10.5psi @17:03:05.69 SS.reconfigure :inhaling 1.06ml/s @17:03:05.74 SS.seek 25ml @17:03:07.21 Stroke completed -- waiting for pressure to equalize ... @17:03:07.22 SS.reconfigure :exhale @17:03:07.26 SS.hold @17:03:13.66 Intake.select :close @17:03:13.81 SSV.dial :exhaust @17:03:14.57 Exhaust.select :open @17:03:14.68 SS.seek 0ml @17:03:23.55 Exhaust.select :close @17:03:23.65 SSV.dial CBV @17:03:23.68
CBV.dial SSV @17:03:24.43 Allowing 130s for 25.0ml of remaining 725.0ml @17:03:24.44 Intake.select :open @17:03:26.59 Intake @15.3psia, vacuum from 9.8 to 12.8psi @17:03:26.60 SS.reconfigure :inhaling 1.06ml/s @17:03:26.65 SS.seek 25ml @17:03:52.69 Stroke completed -- waiting for pressure to equalize ... @17:03:52.70 SS.reconfigure :exhale @17:03:52.74 SS.hold @17:04:01.20 Intake.select :close @17:04:01.34 SSV.dial :exhaust @17:04:02.08 Exhaust.select :open @17:04:02.19 SS.seek 0ml @17:04:10.86 Exhaust.select :close @17:04:10.97 SSV.dial CBV @17:04:11.02
CBV.dial SSV @17:04:11.72 Allowing 130s for 25.0ml of remaining 700.0ml @17:04:11.73 Intake.select :open @17:04:13.89 Intake @15.4psia, vacuum from 9.9 to 12.9psi @17:04:13.91 SS.reconfigure :inhaling 1.06ml/s @17:04:13.95 SS.seek 25ml @17:04:40.14 Stroke completed -- waiting for pressure to equalize ... @17:04:40.15 SS.reconfigure :exhale @17:04:40.19 SS.hold @17:04:49.86 Intake.select :close @17:04:49.99 SSV.dial :exhaust @17:04:50.76 Exhaust.select :open @17:04:50.87 SS.seek 0ml @17:04:59.57 Exhaust.select :close @17:04:59.67 SSV.dial CBV @17:04:59.69
CBV.dial SSV @17:05:00.46 Allowing 130s for 25.0ml of remaining 675.0ml @17:05:00.47 Intake.select :open @17:05:02.62 Intake @15.4psia, vacuum from 9.9 to 12.9psi @17:05:02.63 SS.reconfigure :inhaling 1.06ml/s @17:05:02.68 SS.seek 25ml @17:05:26.18 Vacuum too high -- waiting up to 40 seconds @17:05:26.19 SS.reconfigure :exhale @17:05:26.23 SS.hold @17:05:28.71 Intake @13.3psia, vacuum from 7.8 to 10.8psi @17:05:28.73 SS.reconfigure :inhaling 0.95ml/s @17:05:28.77 SS.seek 25ml @17:05:33.03 Stroke completed -- waiting for pressure to equalize ... @17:05:33.04 SS.reconfigure :exhale @17:05:33.08 SS.hold @17:05:39.47 Intake.select :close @17:05:39.61 SSV.dial :exhaust @17:05:40.36 Exhaust.select :open @17:05:40.47 SS.seek 0ml @17:05:49.14 Exhaust.select :close @17:05:49.25 SSV.dial CBV @17:05:49.30
CBV.dial SSV @17:05:50.02 Allowing 130s for 25.0ml of remaining 650.0ml @17:05:50.03 Intake.select :open @17:05:52.18 Intake @15.4psia, vacuum from 9.9 to 12.9psi @17:05:52.20 SS.reconfigure :inhaling 0.95ml/s @17:05:52.24 SS.seek 25ml @17:06:21.45 Stroke completed -- waiting for pressure to equalize ... @17:06:21.46 SS.reconfigure :exhale @17:06:21.50 SS.hold @17:06:31.16 Intake.select :close @17:06:31.29 SSV.dial :exhaust @17:06:32.06 Exhaust.select :open @17:06:32.16 SS.seek 0ml @17:06:40.97 Exhaust.select :close @17:06:41.07 SSV.dial CBV @17:06:41.10
CBV.dial SSV @17:06:41.86 Allowing 130s for 25.0ml of remaining 625.0ml @17:06:41.87 Intake.select :open @17:06:44.03 Intake @15.4psia, vacuum from 9.9 to 12.9psi @17:06:44.04 SS.reconfigure :inhaling 0.95ml/s @17:06:44.08 SS.seek 25ml @17:07:12.62 Stroke completed -- waiting for pressure to equalize ... @17:07:12.63 SS.reconfigure :exhale @17:07:12.68 SS.hold @17:07:23.79 Intake.select :close @17:07:23.93 SSV.dial :exhaust @17:07:24.69 Exhaust.select :open @17:07:24.80 SS.seek 0ml @17:07:33.44 Exhaust.select :close @17:07:33.54 SSV.dial CBV @17:07:33.56
CBV.dial SSV @17:07:34.33 Allowing 130s for 25.0ml of remaining 600.0ml @17:07:34.34 Intake.select :open @17:07:36.49 Intake @15.3psia, vacuum from 9.8 to 12.8psi @17:07:36.51 SS.reconfigure :inhaling 0.95ml/s @17:07:36.55 SS.seek 25ml @17:08:02.38 Vacuum too high -- waiting up to 40 seconds @17:08:02.39 SS.reconfigure :exhale @17:08:02.43 SS.hold @17:08:07.29 Intake @13.8psia, vacuum from 8.3 to 11.3psi @17:08:07.30 SS.reconfigure :inhaling 0.87ml/s @17:08:07.34 SS.seek 25ml @17:08:11.39 Stroke completed -- waiting for pressure to equalize ... @17:08:11.40 SS.reconfigure :exhale @17:08:11.44 SS.hold @17:08:18.40 Intake.select :close @17:08:18.54 SSV.dial :exhaust @17:08:19.30 Exhaust.select :open @17:08:19.40 SS.seek 0ml @17:08:28.12 Exhaust.select :close @17:08:28.23 SSV.dial CBV @17:08:28.25
CBV.dial SSV @17:08:29.02 Allowing 130s for 25.0ml of remaining 575.0ml @17:08:29.03 Intake.select :open @17:08:31.18 Intake @15.4psia, vacuum from 9.9 to 12.9psi @17:08:31.20 SS.reconfigure :inhaling 0.87ml/s @17:08:31.24 SS.seek 25ml @17:09:02.01 Vacuum too high -- waiting up to 40 seconds @17:09:02.02 SS.reconfigure :exhale @17:09:02.06 SS.hold @17:09:07.67 Intake @14.1psia, vacuum from 8.6 to 11.6psi @17:09:07.69 SS.reconfigure :inhaling 0.78ml/s @17:09:07.73 SS.seek 25ml @17:09:08.94 Stroke completed -- waiting for pressure to equalize ... @17:09:08.95 SS.reconfigure :exhale @17:09:08.99 SS.hold @17:09:14.63 Intake.select :close @17:09:14.77 SSV.dial :exhaust @17:09:15.53 Exhaust.select :open @17:09:15.64 SS.seek 0ml @17:09:24.59 Exhaust.select :close @17:09:24.69 SSV.dial CBV @17:09:24.72
CBV.dial SSV @17:09:25.47 Allowing 130s for 25.0ml of remaining 550.0ml @17:09:25.48 Intake.select :open @17:09:27.63 Intake @15.6psia, vacuum from 10.0 to 13.0psi @17:09:27.65 SS.reconfigure :inhaling 0.78ml/s @17:09:27.69 SS.seek 25ml @17:10:02.20 Stroke completed -- waiting for pressure to equalize ... @17:10:02.21 SS.reconfigure :exhale @17:10:02.25 SS.hold @17:10:13.29 Intake.select :close @17:10:13.42 SSV.dial :exhaust @17:10:14.17 Exhaust.select :open @17:10:14.28 SS.seek 0ml @17:10:22.98 Exhaust.select :close @17:10:23.09 SSV.dial CBV @17:10:23.11
CBV.dial SSV @17:10:23.86 Allowing 130s for 25.0ml of remaining 525.0ml @17:10:23.87 Intake.select :open @17:10:26.02 Intake @15.5psia, vacuum from 10.0 to 13.0psi @17:10:26.04 SS.reconfigure :inhaling 0.78ml/s @17:10:26.08 SS.seek 25ml @17:11:01.01 Stroke completed -- waiting for pressure to equalize ... @17:11:01.02 SS.reconfigure :exhale @17:11:01.06 SS.hold @17:11:13.60 Intake.select :close @17:11:13.74 SSV.dial :exhaust @17:11:14.95 Exhaust.select :open @17:11:15.06 SS.seek 0ml @17:11:23.86 Exhaust.select :close @17:11:23.96 SSV.dial CBV @17:11:23.98
CBV.dial SSV @17:11:24.75 Allowing 130s for 25.0ml of remaining 500.0ml @17:11:24.76 Intake.select :open @17:11:26.91 Intake @15.5psia, vacuum from 10.0 to 13.0psi @17:11:26.93 SS.reconfigure :inhaling 0.78ml/s @17:11:26.97 SS.seek 25ml @17:11:54.32 Vacuum too high -- waiting up to 40 seconds @17:11:54.33 SS.reconfigure :exhale @17:11:54.37 SS.hold @17:12:00.00 Intake @14.4psia, vacuum from 8.9 to 11.9psi @17:12:00.01 SS.reconfigure :inhaling 0.70ml/s @17:12:00.05 SS.seek 25ml @17:12:08.79 Stroke completed -- waiting for pressure to equalize ... @17:12:08.80 SS.reconfigure :exhale @17:12:08.84 SS.hold @17:12:17.29 Intake.select :close @17:12:17.44 SSV.dial :exhaust @17:12:18.20 Exhaust.select :open @17:12:18.31 SS.seek 0ml @17:12:27.07 Exhaust.select :close @17:12:27.18 SSV.dial CBV @17:12:27.23
CBV.dial SSV @17:12:27.95 Allowing 130s for 25.0ml of remaining 475.0ml @17:12:27.96 Intake.select :open @17:12:30.11 Intake @15.6psia, vacuum from 10.0 to 13.0psi @17:12:30.13 SS.reconfigure :inhaling 0.70ml/s @17:12:30.17 SS.seek 25ml @17:13:04.34 Vacuum too high -- waiting up to 40 seconds @17:13:04.35 SS.reconfigure :exhale @17:13:04.40 SS.hold @17:13:10.87 Intake @14.5psia, vacuum from 9.0 to 12.0psi @17:13:10.89 SS.reconfigure :inhaling 0.63ml/s @17:13:10.93 SS.seek 25ml @17:13:16.31 Stroke completed -- waiting for pressure to equalize ... SS.reconfigure :exhale @17:13:16.35 SS.hold @17:13:23.70 Intake.select :close @17:13:23.84 SSV.dial :exhaust @17:13:24.59 Exhaust.select :open @17:13:24.70 SS.seek 0ml @17:13:33.68 Exhaust.select :close @17:13:33.78 SSV.dial CBV @17:13:33.81
CBV.dial SSV @17:13:34.57 Allowing 130s for 25.0ml of remaining 450.0ml @17:13:34.58 Intake.select :open @17:13:36.74 Intake @15.6psia, vacuum from 10.0 to 13.0psi @17:13:36.76 SS.reconfigure :inhaling 0.63ml/s @17:13:36.80 SS.seek 25ml @17:14:18.31 Vacuum too high -- waiting up to 40 seconds @17:14:18.32 SS.reconfigure :exhale @17:14:18.36 SS.hold @17:14:25.21 Intake @14.6psia, vacuum from 9.1 to 12.1psi @17:14:25.23 SS.reconfigure :inhaling 0.57ml/s @17:14:25.27 SS.seek 25ml @17:14:25.32 Stroke completed -- waiting for pressure to equalize ... @17:14:25.33 SS.reconfigure :exhale @17:14:25.37 SS.hold @17:14:31.86 Intake.select :close @17:14:32.00 SSV.dial :exhaust @17:14:32.76 Exhaust.select :open @17:14:32.87 SS.seek 0ml @17:14:41.57 Exhaust.select :close @17:14:41.68 SSV.dial CBV @17:14:41.70
CBV.dial SSV @17:14:42.46 Allowing 130s for 25.0ml of remaining 425.0ml @17:14:42.47 Intake.select :open @17:14:44.63 Intake @15.6psia, vacuum from 10.0 to 13.0psi @17:14:44.64 SS.reconfigure :inhaling 0.57ml/s @17:14:44.68 SS.seek 25ml @17:15:31.15 Stroke completed -- waiting for pressure to equalize ... @17:15:31.16 SS.reconfigure :exhale @17:15:31.20 SS.hold @17:15:45.44 Intake.select :close @17:15:45.57 SSV.dial :exhaust @17:15:46.34 Exhaust.select :open @17:15:46.44 SS.seek 0ml @17:15:55.21 Exhaust.select :close @17:15:55.31 SSV.dial CBV @17:15:55.33
CBV.dial SSV @17:15:56.10 Allowing 130s for 25.0ml of remaining 400.0ml @17:15:56.11 Intake.select :open @17:15:58.26 Intake @15.6psia, vacuum from 10.0 to 13.0psi @17:15:58.28 SS.reconfigure :inhaling 0.57ml/s @17:15:58.32 SS.seek 25ml @17:16:44.66 Stroke completed -- waiting for pressure to equalize ... @17:16:44.67 SS.reconfigure :exhale @17:16:44.71 SS.hold @17:16:59.04 Intake.select :close @17:16:59.17 SSV.dial :exhaust @17:16:59.93 Exhaust.select :open @17:17:00.04 SS.seek 0ml @17:17:08.74 Exhaust.select :close @17:17:08.84 SSV.dial CBV @17:17:08.86
CBV.dial SSV @17:17:09.63 Allowing 130s for 25.0ml of remaining 375.0ml @17:17:09.64 Intake.select :open @17:17:11.79 Intake @15.6psia, vacuum from 10.0 to 13.0psi @17:17:11.81 SS.reconfigure :inhaling 0.57ml/s @17:17:11.85 SS.seek 25ml @17:17:51.21 Vacuum too high -- waiting up to 40 seconds @17:17:51.22 SS.reconfigure :exhale @17:17:51.26 SS.hold @17:17:58.72 Intake @14.9psia, vacuum from 9.4 to 12.4psi @17:17:59.21 SS.reconfigure :inhaling 0.51ml/s @17:17:59.25 SS.seek 25ml @17:18:07.22 Stroke completed -- waiting for pressure to equalize ... @17:18:07.23 SS.reconfigure :exhale @17:18:07.27 SS.hold @17:18:16.93 Intake.select :close @17:18:17.06 SSV.dial :exhaust @17:18:17.82 Exhaust.select :open @17:18:17.93 SS.seek 0ml @17:18:26.89 Exhaust.select :close @17:18:27.00 SSV.dial CBV @17:18:27.02
CBV.dial SSV @17:18:27.79 Allowing 130s for 25.0ml of remaining 350.0ml @17:18:27.80 Intake.select :open @17:18:29.95 Intake @15.7psia, vacuum from 10.0 to 13.0psi @17:18:29.97 SS.reconfigure :inhaling 0.51ml/s @17:18:30.01 SS.seek 25ml @17:19:18.00 Vacuum too high -- waiting up to 40 seconds @17:19:18.01 SS.reconfigure :exhale @17:19:18.05 SS.hold @17:19:26.48 Intake @15.0psia, vacuum from 9.5 to 12.5psi @17:19:26.50 SS.reconfigure :inhaling 0.47ml/s @17:19:26.54 SS.seek 25ml @17:19:30.16 Stroke completed -- waiting for pressure to equalize ... @17:19:30.17 SS.reconfigure :exhale @17:19:30.21 SS.hold @17:19:39.85 Intake.select :close @17:19:39.99 SSV.dial :exhaust @17:19:40.76 Exhaust.select :open @17:19:40.87 SS.seek 0ml @17:19:49.69 Exhaust.select :close @17:19:49.79 SSV.dial CBV @17:19:49.82
CBV.dial SSV @17:19:50.58 Allowing 130s for 25.0ml of remaining 325.0ml @17:19:50.59 Intake.select :open @17:19:52.75 Intake @15.7psia, vacuum from 10.0 to 13.0psi @17:19:52.76 SS.reconfigure :inhaling 0.47ml/s @17:19:52.81 SS.seek 25ml @17:20:48.88 Stroke completed -- waiting for pressure to equalize ... @17:20:48.89 SS.reconfigure :exhale @17:20:48.93 SS.hold @17:21:05.24 Intake.select :close @17:21:05.38 SSV.dial :exhaust @17:21:06.13 Exhaust.select :open @17:21:06.23 SS.seek 0ml @17:21:14.94 Exhaust.select :close @17:21:15.04 SSV.dial CBV @17:21:15.06
CBV.dial SSV @17:21:15.83 Allowing 130s for 25.0ml of remaining 300.0ml @17:21:15.84 Intake.select :open @17:21:17.99 Intake @15.7psia, vacuum from 10.0 to 13.0psi @17:21:18.01 SS.reconfigure :inhaling 0.47ml/s @17:21:18.51 SS.seek 25ml @17:22:08.09 Vacuum too high -- waiting up to 40 seconds @17:22:08.10 SS.reconfigure :exhale @17:22:08.14 SS.hold @17:22:17.76 Intake @15.0psia, vacuum from 9.5 to 12.5psi @17:22:17.78 SS.reconfigure :inhaling 0.43ml/s @17:22:17.82 SS.seek 25ml @17:22:25.45 Stroke completed -- waiting for pressure to equalize ... @17:22:25.46 SS.reconfigure :exhale @17:22:25.50 SS.hold @17:22:36.53 Intake.select :close @17:22:36.66 SSV.dial :exhaust @17:22:37.42 Exhaust.select :open @17:22:37.53 SS.seek 0ml @17:22:46.51 Exhaust.select :close @17:22:46.62 SSV.dial CBV @17:22:46.64
CBV.dial SSV @17:22:47.39 Allowing 130s for 25.0ml of remaining 275.0ml @17:22:47.40 Intake.select :open @17:22:49.55 Intake @15.7psia, vacuum from 10.0 to 13.0psi @17:22:49.57 SS.reconfigure :inhaling 0.43ml/s @17:22:49.61 SS.seek 25ml @17:23:52.20 Stroke completed -- waiting for pressure to equalize ... @17:23:52.21 SS.reconfigure :exhale @17:23:52.25 SS.hold @17:24:10.47 Intake.select :close @17:24:10.61 SSV.dial :exhaust @17:24:11.34 Exhaust.select :open @17:24:11.45 SS.seek 0ml @17:24:20.45 Exhaust.select :close @17:24:20.60 SSV.dial CBV @17:24:20.62
CBV.dial SSV @17:24:21.37 Allowing 130s for 25.0ml of remaining 250.0ml @17:24:21.38 Intake.select :open @17:24:23.54 Intake @15.8psia, vacuum from 10.0 to 13.0psi @17:24:23.56 SS.reconfigure :inhaling 0.43ml/s @17:24:23.60 SS.seek 25ml @17:25:16.43 Vacuum too high -- waiting up to 40 seconds SS.reconfigure :exhale @17:25:16.47 SS.hold @17:25:25.01 Intake @15.1psia, vacuum from 9.6 to 12.6psi @17:25:25.03 SS.reconfigure :inhaling 0.38ml/s @17:25:25.07 SS.seek 25ml @17:25:37.32 Stroke completed -- waiting for pressure to equalize ... @17:25:37.33 SS.reconfigure :exhale @17:25:37.37 SS.hold @17:25:51.84 Intake.select :close @17:25:51.98 SSV.dial :exhaust @17:25:52.73 Exhaust.select :open @17:25:52.84 SS.seek 0ml @17:26:01.84 Exhaust.select :close @17:26:01.94 SSV.dial CBV @17:26:01.96
CBV.dial SSV @17:26:02.73 Allowing 130s for 25.0ml of remaining 225.0ml @17:26:02.74 Intake.select :open @17:26:04.90 Intake @15.8psia, vacuum from 10.0 to 13.0psi @17:26:04.91 SS.reconfigure :inhaling 0.38ml/s @17:26:04.95 SS.seek 25ml @17:27:17.63 Stroke completed -- waiting for pressure to equalize ... @17:27:17.64 SS.reconfigure :exhale @17:27:17.68 SS.hold @17:27:37.93 Intake.select :close @17:27:38.07 SSV.dial :exhaust @17:27:38.83 Exhaust.select :open @17:27:38.94 SS.seek 0ml @17:27:47.87 Exhaust.select :close @17:27:47.97 SSV.dial CBV @17:27:47.99
CBV.dial SSV @17:27:48.76 Allowing 130s for 25.0ml of remaining 200.0ml @17:27:48.77 Intake.select :open @17:27:50.92 Intake @15.7psia, vacuum from 10.0 to 13.0psi @17:27:50.94 SS.reconfigure :inhaling 0.38ml/s @17:27:50.98 SS.seek 25ml @17:29:04.51 Stroke completed -- waiting for pressure to equalize ... @17:29:04.52 SS.reconfigure :exhale @17:29:04.56 SS.hold @17:29:26.86 Intake.select :close @17:29:26.99 SSV.dial :exhaust @17:29:27.74 Exhaust.select :open @17:29:27.85 SS.seek 0ml @17:29:36.38 Exhaust.select :close @17:29:36.48 SSV.dial CBV @17:29:36.51
CBV.dial SSV @17:29:37.28 Allowing 130s for 25.0ml of remaining 175.0ml @17:29:37.29 Intake.select :open @17:29:39.44 Intake @15.8psia, vacuum from 10.0 to 13.0psi @17:29:39.46 SS.reconfigure :inhaling 0.38ml/s @17:29:39.50 SS.seek 25ml @17:30:37.08 Vacuum too high -- waiting up to 40 seconds @17:30:37.09 SS.reconfigure :exhale @17:30:37.13 SS.hold @17:30:48.69 Intake @15.4psia, vacuum from 9.9 to 12.9psi @17:30:48.70 SS.reconfigure :inhaling 0.34ml/s @17:30:48.75 SS.seek 25ml @17:31:07.46 Stroke completed -- waiting for pressure to equalize ... @17:31:07.47 SS.reconfigure :exhale @17:31:07.51 SS.hold @17:31:25.83 Intake.select :close @17:31:25.96 SSV.dial :exhaust @17:31:26.72 Exhaust.select :open @17:31:26.83 SS.seek 0ml @17:31:35.43 Exhaust.select :close @17:31:35.53 SSV.dial CBV @17:31:35.55
CBV.dial SSV @17:31:36.32 Allowing 130s for 25.0ml of remaining 150.0ml @17:31:36.33 Intake.select :open @17:31:38.48 Intake @15.8psia, vacuum from 10.0 to 13.0psi @17:31:38.50 SS.reconfigure :inhaling 0.34ml/s @17:31:38.54 SS.seek 25ml @17:33:07.16 Stroke completed -- waiting for pressure to equalize ... @17:33:07.17 SS.reconfigure :exhale @17:33:07.21 SS.hold @17:33:31.56 Intake.select :close @17:33:31.69 SSV.dial :exhaust @17:33:32.46 Exhaust.select :open @17:33:32.56 SS.seek 0ml @17:33:41.41 Exhaust.select :close @17:33:41.51 SSV.dial CBV @17:33:41.53
CBV.dial SSV @17:33:42.31 Allowing 130s for 25.0ml of remaining 125.0ml @17:33:42.32 Intake.select :open @17:33:44.48 Intake @15.7psia, vacuum from 10.0 to 13.0psi @17:33:44.50 SS.reconfigure :inhaling 0.34ml/s @17:33:44.54 SS.seek 25ml @17:35:12.78 Stroke completed -- waiting for pressure to equalize ... @17:35:12.79 SS.reconfigure :exhale @17:35:12.83 SS.hold @17:35:41.26 Intake.select :close @17:35:41.39 SSV.dial :exhaust @17:35:42.16 Exhaust.select :open @17:35:42.26 SS.seek 0ml @17:35:50.84 Exhaust.select :close @17:35:50.95 SSV.dial CBV @17:35:50.98
CBV.dial SSV @17:35:51.74 Allowing 130s for 25.0ml of remaining 100.0ml @17:35:51.75 Intake.select :open @17:35:54.30 Intake @15.8psia, vacuum from 10.0 to 13.0psi @17:35:54.38 SS.reconfigure :inhaling 0.34ml/s @17:35:54.43 SS.seek 25ml @17:37:10.29 Vacuum too high -- waiting up to 40 seconds @17:37:10.30 SS.reconfigure :exhale @17:37:10.34 SS.hold @17:37:24.58 Intake @15.5psia, vacuum from 10.0 to 13.0psi @17:37:24.60 SS.reconfigure :inhaling 0.31ml/s @17:37:24.64 SS.seek 25ml @17:37:41.12 Stroke completed -- waiting for pressure to equalize ... @17:37:41.13 SS.reconfigure :exhale @17:37:41.17 SS.hold @17:38:01.42 Intake.select :close @17:38:01.55 SSV.dial :exhaust @17:38:02.32 Exhaust.select :open @17:38:02.42 SS.seek 0ml @17:38:11.20 Exhaust.select :close @17:38:11.31 SSV.dial CBV @17:38:11.35
CBV.dial SSV @17:38:12.06 Allowing 130s for 25.0ml of remaining 75.0ml @17:38:12.07 Intake.select :open @17:38:14.22 Intake @15.2psia, vacuum from 9.7 to 12.7psi @17:38:14.24 SS.reconfigure :inhaling 0.31ml/s @17:38:14.28 SS.seek 25ml @17:39:54.43 Stroke completed -- waiting for pressure to equalize ... @17:39:54.44 SS.reconfigure :exhale @17:39:54.48 SS.hold @17:40:22.08 SS.reconfigure :default @17:40:22.13 SS.hold @17:40:22.24 Intake.select :close @17:40:22.37 ShallowSampler::Clogged in MAIN -- Filtering too Slowly after 950.0ml @17:40:22.38 SSV.dial :exhaust @17:40:23.12 Exhaust.select :open @17:40:23.23 SS.reconfigure :exhale @17:40:23.27 SS.seek 0ml @17:40:32.26 Exhaust.select :close @17:40:32.36 Sampled 950.0ml @17:40:32.38 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) Intake.select :close @17:40:32.44 CSV.dial :waste @17:40:32.48 CS.seek 0ml @17:40:32.59 CSV.dial :air @17:40:32.61 SSV.dial CBV,avoiding: :exhaust @17:40:32.65
CBV.dial SSV @17:40:33.40 CS.seek 10ml @17:40:34.53
CTV.dial :air @17:40:35.33 delay 15 seconds @17:40:50.35 SSV.dial :spit,avoiding: :exhaust @17:40:51.66 CTV.dial CSV,avoiding: :intake @17:40:51.69
SS.seek 0ml @17:40:51.80 SSV.dial CBV,avoiding: :exhaust @17:40:57.79 CBV.dial between SSV and waste2 @17:40:58.18 CTV.dial CSV,avoiding: :air @17:40:58.20
CSV.dial CTV @17:40:58.65 CS.reconfigure :pressurize @17:40:58.69 CS.seek 0ml @17:41:16.36 BubblePoint: Intake @38.8psia, Outlet @14.5psia CBV.dial SSV @17:41:16.69 SS.reconfigure :default @17:41:16.73 SS.seek 10ml @17:41:22.26 delay 15 seconds @17:41:37.38 Evacuated: Intake @17.0psia, Outlet @8.1psia @17:41:37.39 SSV.dial :spit,avoiding: :exhaust @17:41:38.72 SS.reconfigure :exhale @17:41:38.76 SS.seek 0ml @17:41:44.00 SS.reconfigure :fast @17:41:44.04 SS.seek 5ml @17:41:46.86 SSV.dial CBV,avoiding: :exhaust @17:41:46.88
CBV.dial SSV @17:41:48.22 CTV.dial :air,avoiding: :intake @17:41:49.58 CSV.dial CTV @17:41:49.61
SS.seek 10ml @17:41:50.60 CSV.dial :air @17:41:51.05 CS.reconfigure :fast @17:41:51.09 CS.seek 0ml @17:41:51.98
SSV.dial :spit,avoiding: :exhaust @17:41:53.31 SS.reconfigure :exhale @17:41:53.36 SS.seek 0ml @17:41:58.57 SS.reconfigure :fast @17:41:58.61 SS.seek 5ml @17:41:59.45 CS.stop @17:42:01.46 SSV.dial CBV,avoiding: :exhaust @17:42:01.47
CBV.dial SSV @17:42:03.82 SSV.dial :spit,avoiding: :exhaust @17:42:05.13 SS.reconfigure :default @17:42:05.17 SS.seek 0ml @17:42:08.40 SS.stop @17:42:08.44 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @17:42:08.45 Intake.select :close @17:42:08.52 CSV.dial :waste @17:42:08.64 SSV.dial CBV,avoiding: :exhaust @17:42:08.66
CBV.dial SSV @17:42:09.27 CS.seek 0ml @17:42:09.33 CSV.dial :air @17:42:10.00
CTV.dial :air @17:42:10.01 delay 15 seconds @17:42:10.07 CS.seek 10ml @17:42:25.03
SSV.dial :spit,avoiding: :exhaust @17:42:26.36 CTV.dial CSV,avoiding: :intake @17:42:26.39
SS.reconfigure :exhale @17:42:26.48 SS.seek 0ml @17:42:26.89 SSV.dial CBV,avoiding: :exhaust @17:42:34.35 CBV.dial between SSV and waste2 @17:42:34.73 CTV.dial CSV,avoiding: :air @17:42:34.75
CSV.dial CTV @17:42:35.20 CS.reconfigure :pressurize @17:42:35.25 CS.seek 0ml @17:42:56.48 BubblePoint: Intake @37.8psia, Outlet @13.6psia @17:42:56.49 CBV.dial SSV @17:42:56.81 SS.reconfigure :default @17:42:56.85 SS.seek 10ml @17:43:02.42 delay 15 seconds @17:43:17.53 Evacuated: Intake @18.7psia, Outlet @11.0psia @17:43:17.54 SSV.dial :spit,avoiding: :exhaust @17:43:18.86 SS.reconfigure :exhale @17:43:18.90 SS.seek 0ml @17:43:24.17 SS.reconfigure :fast @17:43:24.21 SS.seek 5ml @17:43:26.99 SSV.dial CBV,avoiding: :exhaust @17:43:27.00
CBV.dial SSV @17:43:28.33 CTV.dial :air,avoiding: :intake @17:43:29.68 CSV.dial CTV @17:43:29.72
SS.seek 10ml @17:43:30.71 CSV.dial :air @17:43:31.19 CS.reconfigure :fast @17:43:31.23 CS.seek 0ml @17:43:32.20
SSV.dial :spit,avoiding: :exhaust @17:43:34.45 SS.reconfigure :exhale @17:43:34.50 SS.seek 0ml @17:43:36.41 CS.stop @17:43:39.49
SS.reconfigure :fast @17:43:39.53 SS.seek 5ml @17:43:42.30 SSV.dial CBV,avoiding: :exhaust @17:43:42.32
CBV.dial SSV @17:43:44.64 SSV.dial :spit,avoiding: :exhaust @17:43:45.95 SS.reconfigure :default @17:43:45.99 SS.seek 0ml @17:43:49.21 SS.stop @17:43:49.68 Lysing: Adding 2.0ml AqMeOH @17:43:49.69 CBV.dial between CSV and waste1,avoiding: :DBV @17:43:51.18 CTV.dial CSV @17:43:52.52 CSV.dial CSR @17:43:53.31 Pulling 1ml of CSR.AqMeOH @17:43:53.32 CSR.select :AqMeOH @17:43:53.43 CS.reconfigure :default @17:43:53.47 CS.seek 1ml @17:43:57.07 delay 5 seconds @17:44:02.09 CSR.select :air @17:44:02.24 CS.seek 6ml @17:44:15.82 delay 2 seconds @17:44:17.84 CSV.dial :waste2 @17:44:18.28 CS.reconfigure :fast @17:44:18.32 CS.seek 0ml @17:44:32.85 CSV.dial CSR @17:44:33.36 Pulling 2ml of CSR.AqMeOH @17:44:33.37 CSR.select :AqMeOH @17:44:33.48 CS.reconfigure :default @17:44:33.52 CS.seek 2ml @17:44:39.48 delay 5 seconds @17:44:44.50 CSR.select :air @17:44:44.65 CS.seek 5ml @17:44:53.23 delay 2 seconds @17:44:55.24 CSV.dial CTV @17:44:55.69 CS.seek 1ml @17:45:07.02 Before lysing Collection Puck Heater at 13.4C/38.5psia @17:45:07.04 Heating Puck.from 2, :sh1 in Collection Clamp to 70C for 10:00 @17:45:07.05 CH.seek 70C,8:00 @17:47:09.46 CH.hold 10:00,70C @17:47:09.62 Begun lysing Collection Puck Heater at 68C/47.6psia @17:57:09.65 Ended lysing Collection Puck Heater at 70C/47.5psia @17:57:09.66 CS.seek 0ml @17:57:13.00 CTV.dial between spare and air,avoiding: :intake @17:57:14.05 CSV.dial :air @17:57:14.49 CS.seek 5ml @17:57:28.09 CSV.dial CTV @17:57:28.53 delay 2 seconds @17:57:30.55 CSV.dial :air @17:57:31.00 delay 2 seconds @17:57:33.01 CSV.dial CTV @17:57:33.47 delay 2 seconds @17:57:35.48 CSV.dial :air @17:57:35.94 delay 2 seconds @17:57:37.96 CS.seek 0ml @17:57:51.58 Puck @75.7psia after final lysis pressurization @17:57:51.59 Recovering Lysate into Collection syringe @17:57:51.60 CSV.dial CBV @17:57:52.62 CBV.dial CSV @17:57:52.99 CS.seek 5.001ml @17:58:06.56 delay 5 seconds @17:58:11.58 CTV.dial :air @17:58:12.10 CS.seek 7.001ml @17:58:17.96 delay 5 seconds @17:58:22.98 CSV.dial :air2 @17:58:23.43 CS.seek 10ml @17:58:31.97 CSV.dial between air and CTV,avoiding: CBV @17:58:33.13 Lysing Complete @17:58:33.19 Storing sh1 puck in Collection Clamp to tube 1 @17:58:33.36 SC.reconfigure :tight @17:58:33.40 SC.seek 1 @17:58:36.94 SC.configure :limp @17:58:37.14 Elbow.reconfigure :tight @17:58:37.18 Elbow.seek 1 @17:58:38.97 Forearm.reconfigure :tight @17:58:39.01 Forearm.seek 1 + 450 counts @17:58:40.07 SE.reconfigure :pick @17:58:40.12 SE.seek :beamBreak @17:58:43.01 SE.configure :default @17:58:43.14 Elbow.reconfigure :limp @17:58:43.19 Forearm.reconfigure :loose @17:58:43.23 Forearm.seek 1 @17:58:43.43 Elbow.hold @17:58:43.49 Elbow.reconfigure :tight @17:58:43.58 SE.seek 3.16 Pucks below Top Plate @17:58:44.02 Forearm.force -20 @17:58:44.05 Forearm.seek 1 + 308 counts @17:58:44.33 Forearm.reconfigure :tight @17:58:44.37 Forearm.seek 1 + 450 counts @17:58:44.55 SE.seek 4.16 Pucks below Top Plate @17:58:45.04 Forearm.reconfigure :loose @17:58:45.09 Forearm.seek :retract @17:58:46.02 Elbow.seek CC @17:58:47.72 Elbow.reconfigure :loose @17:58:47.81 Forearm.seek CC @17:58:48.79 Hand.close @17:58:49.40 CC.seek :open @17:58:54.15 Forearm.seek :retract @17:58:55.12 Elbow.reconfigure :tight @17:58:55.16 Elbow.seek 1 @17:58:56.98 Elbow.reconfigure :limp @17:58:57.02 Forearm.seek 1 @17:58:58.01 Elbow.hold @17:58:58.07 Elbow.reconfigure :tight @17:58:58.11 Hand.open @17:58:58.89 Forearm.force -20 @17:58:58.92 Forearm.seek 1 + 310 counts @17:58:59.20 Forearm.reconfigure :tight @17:58:59.24 Forearm.seek 1 + 450 counts @17:58:59.48 SE.seek :down @17:59:00.60
Forearm.reconfigure :loose @17:59:00.65 Forearm.seek :retract @17:59:01.91 SE.stop @17:59:01.95
Tranferring DA @17:59:01.96 Beginning samplePassC @17:59:01.97 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @17:59:02.15 Elbow.seek FlushPuck::Garage @17:59:03.92 Elbow.reconfigure :loose @17:59:04.02 Forearm.seek :garage @17:59:04.98 Hand.close @17:59:05.64 Forearm.seek :retract @17:59:06.67 Elbow.reconfigure :tight @17:59:06.71 Elbow.seek CC @17:59:09.72 Elbow.reconfigure :loose @17:59:09.81 Forearm.reconfigure :tight @17:59:09.85 Forearm.seek CC @17:59:11.80 CC.seek :closed @17:59:16.31 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.31 @17:59:16.38 Hand.open @17:59:17.09 Forearm.reconfigure :loose @17:59:17.13 Forearm.seek :retract @17:59:18.10 PSV.dial PSR @17:59:19.51 Pulling 1.5ml of PSR.flush @17:59:19.52 PSR.select :flush @17:59:19.63 PS.seek 1.5ml @17:59:35.75 delay 5 seconds @17:59:40.77 PSV.dial :waste1 @17:59:41.24 PS.seek 0ml @17:59:57.37 PSV.dial PSR @17:59:57.84 PSR.select :air @17:59:57.98 PS.seek 2.5ml @18:00:24.29 PRV.dial CBV,avoiding: :ab2 @18:00:24.31
CBV.dial PRV @18:00:26.00 PSV.dial :mixloop @18:00:27.05 CTV.dial :air,avoiding: :intake @18:00:27.06 PTV.dial :PRVmixing @18:00:28.38 PS.seek 0ml @18:00:54.68 PSV.dial :air @18:00:55.15 PS.seek 2.5ml @18:01:21.56 PSV.dial :mixloop @18:01:22.04 PS.seek 0ml @18:01:48.31 PSV.dial :air @18:01:48.77 PS.seek 2.5ml @18:02:15.15 PSV.dial :mixloop @18:02:15.61 PS.seek 0ml @18:02:42.03 Garaging FlushPuck in Collection Clamp @18:02:42.10 Elbow.reconfigure :tight @18:02:42.14 Elbow.seek CC @18:02:42.58 Elbow.reconfigure :loose @18:02:42.67 Forearm.seek CC @18:02:43.69 Hand.close @18:02:44.28 CC.seek :open @18:02:48.89 Forearm.seek :retract @18:02:49.87 Elbow.reconfigure :tight @18:02:49.91 Elbow.seek :garage @18:02:52.89 Elbow.reconfigure :loose @18:02:52.98 Forearm.reconfigure :tight @18:02:53.02 Forearm.seek :garage @18:02:54.06 Hand.open @18:02:54.71 Forearm.reconfigure :loose @18:02:54.75 Forearm.seek :retract @18:02:55.79 PSV.dial :mixloop @18:02:55.80 PTV.dial :PRVmixing @18:02:55.81 PRV.dial CBV @18:02:55.84
CBV.dial PRV @18:02:55.85 CTV.dial :air,avoiding: :intake @18:02:55.87 Loading lyfil puck from tube 2 into Collection Clamp @18:02:56.08 SC.reconfigure :tight @18:02:56.13 SC.seek 2 @18:02:59.66 SC.configure :limp @18:02:59.85 Elbow.reconfigure :tight @18:02:59.89 Elbow.seek 2 @18:03:01.93 Forearm.reconfigure :tight @18:03:01.97 Forearm.seek 2 + 450 counts @18:03:02.96 SE.reconfigure :pick @18:03:03.01 SE.seek :beamBreak @18:03:04.35 SE.configure :default @18:03:04.49 Elbow.seek 2 @18:03:04.95 Forearm.seek 2 + 300 counts @18:03:05.13 Forearm.reconfigure :loose @18:03:05.17 Hand.close @18:03:05.79 SE.seek :down @18:03:05.97
Forearm.seek :retract @18:03:07.17 SE.stop @18:03:07.24
Elbow.seek CC @18:03:08.97 Elbow.reconfigure :loose @18:03:09.06 Forearm.reconfigure :tight @18:03:09.10 Forearm.seek CC @18:03:10.38 CC.seek :closed @18:03:14.90 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.02 @18:03:14.91 Hand.open @18:03:15.58 Forearm.reconfigure :loose @18:03:15.62 Forearm.seek :retract @18:03:16.65 CTV.dial CSV,avoiding: :intake @18:03:18.01 CSV.dial CTV @18:03:18.31 PS.seek 2.5ml @18:03:44.68 CS.seek 4.5ml @18:03:59.62 delay 8 seconds @18:04:07.64 PSV.dial :air @18:04:08.11 delay 2 seconds @18:04:10.12 PSV.dial :waste2 @18:04:10.99 PS.seek 0ml @18:04:37.40 CBV.dial between PRV and waste1 @18:04:37.73 CSV.dial :air @18:04:38.18 CS.seek 10ml @18:04:53.14 CSV.dial CTV @18:04:53.59 CS.seek 0ml @18:05:19.96 CBV.dial :waste1 @18:05:20.26 delay 5 seconds @18:05:25.28 CTV.dial :air,avoiding: :intake @18:05:26.69 Storing lyfil puck in Collection Clamp to tube 1 @18:05:26.84 SC.reconfigure :tight @18:05:26.89 SC.seek 1 @18:05:30.43 SC.configure :limp @18:05:30.62 Elbow.reconfigure :tight @18:05:30.66 Elbow.seek 1 @18:05:32.43 Forearm.reconfigure :tight @18:05:32.47 Forearm.seek 1 + 450 counts @18:05:33.50 SE.reconfigure :pick @18:05:33.55 SE.seek :beamBreak @18:05:36.40 SE.configure :default @18:05:36.54 Elbow.reconfigure :limp @18:05:36.58 Forearm.reconfigure :loose @18:05:36.62 Forearm.seek 1 @18:05:36.83 Elbow.hold @18:05:36.89 Elbow.reconfigure :tight @18:05:36.98 SE.seek 4.16 Pucks below Top Plate @18:05:37.41 Forearm.force -20 @18:05:37.44 Forearm.seek 1 + 303 counts @18:05:37.72 Forearm.reconfigure :tight @18:05:37.76 Forearm.seek 1 + 450 counts @18:05:37.94 SE.seek 5.16 Pucks below Top Plate @18:05:38.57 Forearm.reconfigure :loose @18:05:38.62 Forearm.seek :retract @18:05:39.54 Elbow.seek CC @18:05:41.25 Elbow.reconfigure :loose @18:05:41.33 Forearm.seek CC @18:05:42.32 Hand.close @18:05:42.90 CC.seek :open @18:05:47.63 Forearm.seek :retract @18:05:48.62 Elbow.reconfigure :tight @18:05:48.66 Elbow.seek 1 @18:05:50.44 Elbow.reconfigure :limp @18:05:50.49 Forearm.seek 1 @18:05:51.46 Elbow.hold @18:05:51.99 Elbow.reconfigure :tight @18:05:52.04 Hand.open @18:05:52.77 Forearm.force -20 @18:05:52.80 Forearm.seek 1 + 302 counts @18:05:53.09 Forearm.reconfigure :tight @18:05:53.13 Forearm.seek 1 + 450 counts @18:05:53.40 SE.seek :down @18:05:54.52
Forearm.reconfigure :loose @18:05:54.56 Forearm.seek :retract @18:05:55.75 SE.stop @18:05:55.78
PSV.dial :air @18:05:56.53 PS.seek 2.5ml @18:06:22.93 delay 1 second @18:06:23.94 PSV.dial :mixloop @18:06:24.38 delay 1 second @18:06:25.40 PSV.dial :air @18:06:25.98 delay 1 second @18:06:26.99 PSV.dial :mixloop @18:06:27.45 delay 1 second @18:06:28.46 PSV.dial :air @18:06:28.92 delay 1 second @18:06:29.94 PS.seek 0ml @18:06:56.24 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @18:06:56.41 Elbow.seek FlushPuck::Garage @18:06:58.27 Elbow.reconfigure :loose @18:06:58.36 Forearm.seek :garage @18:06:59.36 Hand.close @18:07:00.13 Forearm.seek :retract @18:07:01.08 Elbow.reconfigure :tight @18:07:01.12 Elbow.seek PC @18:07:02.59 Elbow.reconfigure :loose @18:07:02.68 Forearm.reconfigure :tight @18:07:02.72 Forearm.seek PC @18:07:03.89 PC.seek :closed @18:07:08.54 Processing Clamp onSpring/offSpring for 16/26 samples, on/off torque 1.51 @18:07:08.61 Hand.open @18:07:09.33 Forearm.reconfigure :loose @18:07:09.37 Forearm.seek :retract @18:07:10.32 PRV.dial :air @18:07:10.76 PSV.dial :mixloop @18:07:11.28 PS.seek 0.3ml @18:07:15.12 PSV.dial PSR @18:07:16.22 Pulling 0.4ml of PSR.air @18:07:16.23 PSR.select :air @18:07:16.26 PS.seek 0.7ml @18:07:21.21 delay 3 seconds @18:07:24.23 PSR.select :air @18:07:24.25 PS.seek 2.5ml @18:07:43.43 delay 3 seconds @18:07:46.45 PSV.dial :mixloop @18:07:47.51 PTV.dial :mixing @18:07:48.27 PBV.dial :waste2 @18:07:49.22 PS.seek 0ml @18:08:15.59 Garaging FlushPuck in Processing Clamp @18:08:15.65 Elbow.reconfigure :tight @18:08:15.70 Elbow.seek PC @18:08:15.81 Elbow.reconfigure :loose @18:08:15.94 Forearm.seek PC @18:08:16.91 Hand.close @18:08:17.52 PC.seek :open @18:08:22.22 Forearm.seek :retract @18:08:23.16 Elbow.reconfigure :tight @18:08:23.20 Elbow.seek :garage @18:08:24.49 Elbow.reconfigure :loose @18:08:24.59 Forearm.reconfigure :tight @18:08:24.63 Forearm.seek :garage @18:08:25.58 Hand.open @18:08:26.20 Forearm.reconfigure :loose @18:08:26.24 Forearm.seek :retract @18:08:27.68 Loading da puck from tube 2 into Processing Clamp @18:08:27.89 SC.reconfigure :tight @18:08:27.94 SC.seek 2 @18:08:31.48 SC.configure :limp @18:08:31.68 Elbow.reconfigure :tight @18:08:31.72 Elbow.seek 2 @18:08:33.57 Forearm.reconfigure :tight @18:08:33.61 Forearm.seek 2 + 450 counts @18:08:34.64 SE.reconfigure :pick @18:08:34.69 SE.seek :beamBreak @18:08:36.09 SE.configure :default @18:08:36.24 Elbow.seek 2 @18:08:36.41 Forearm.seek 2 + 300 counts @18:08:36.60 Forearm.reconfigure :loose @18:08:36.64 Hand.close @18:08:37.28 SE.seek :down @18:08:37.46
Forearm.seek :retract @18:08:38.29 SE.stop @18:08:38.38
Elbow.seek PC @18:08:41.01 Elbow.reconfigure :loose @18:08:41.10 Forearm.reconfigure :tight @18:08:41.14 Forearm.seek PC @18:08:42.30 PC.seek :closed @18:08:46.93 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.56 @18:08:46.94 Hand.open @18:08:47.58 Forearm.reconfigure :loose @18:08:47.62 Forearm.seek :retract @18:08:48.62 Heating Puck.from 2, :da in Processing Clamp to 28C for 2:00:00 @18:08:48.64 PH.seek 28C,5:00 @18:09:28.48 PH.hold 2:00:00,28C @18:09:28.52 delay 1:30 @18:10:58.56 PSR.select :air @18:10:58.58 PSV.dial PSR @18:10:59.67 Pulling 0.5ml of PSR.wash @18:10:59.68 PSR.select :wash @18:10:59.79 PS.seek 0.5ml @18:11:05.74 delay 5 seconds @18:11:10.76 PSR.select :air @18:11:10.87 PS.seek 2ml @18:11:27.08 PTV.dial :puck @18:11:27.82 PSV.dial PTV @18:11:28.58 PBV.dial :waste1 @18:11:29.99 PS.seek 1.5ml @18:11:35.89 delay 3 seconds @18:11:38.91 PBV.dial between PSV and waste1 @18:11:39.28 PS.seek 0ml @18:11:55.49 delay 5:00 @18:16:55.51 recoverPSRreagent08 @18:16:55.52 PTV.dial :puck @18:16:55.53 PBV.dial :waste1 @18:16:55.90 delay 30 seconds @18:17:25.93 PBV.dial between PSV and waste1 @18:17:26.28 PSR.select :air @18:17:26.30 PSV.dial PSR @18:17:27.07 PS.reconfigure :fast @18:17:27.11 PS.seek 2.5ml @18:17:51.01 PSV.dial PTV @18:17:51.76 delay 5 seconds @18:17:56.78 PSV.dial PSR @18:17:57.55 delay 5 seconds @18:18:02.56 PSV.dial PTV @18:18:03.33 delay 5 seconds @18:18:08.34 PSV.dial :air @18:18:09.70 PS.seek 0ml @18:18:32.92 PTV.dial :PRVmixing,via: PRV @18:18:34.25 PSV.dial :mixloop @18:18:34.68 PRV.dial :ab1 @18:18:35.42 PS.reconfigure :slow @18:18:35.47 PS.seek 0.15ml @18:18:39.11 delay 2 seconds @18:18:41.12 CBV.dial PRV @18:18:41.14
PRV.dial CBV @18:18:41.71 PS.seek 0.35ml @18:18:46.36 delay 2 seconds @18:18:48.37 CBV.dial between PRV and PSV @18:18:48.70 PRV.dial :air @18:18:49.16 PS.seek 0.9ml @18:19:00.92 PRV.dial :waste2 @18:19:01.66 PS.seek 0.5ml @18:19:10.29 addPMLReagent08 @18:19:10.30 PSV.dial :air @18:19:10.77 PS.reconfigure :default @18:19:10.81 PS.seek 2ml @18:19:26.98 PSV.dial :PML @18:19:27.43 PTV.dial :mixing @18:19:28.18 PBV.dial :waste1 @18:19:28.59 PS.seek 1.85ml @18:19:30.92 PBV.dial between PSV and waste1 @18:19:31.28 PS.seek 0ml @18:19:50.98 delay 2 seconds @18:19:53.00 Beginning samplePassC @18:19:53.01 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @18:19:53.17 Elbow.reconfigure :tight @18:19:53.21 Elbow.seek FlushPuck::Garage @18:19:54.55 Elbow.reconfigure :loose @18:19:54.64 Forearm.seek :garage @18:19:55.60 Hand.close @18:19:56.38 Forearm.seek :retract @18:19:57.34 Elbow.reconfigure :tight @18:19:57.38 Elbow.seek CC @18:20:00.33 Elbow.reconfigure :loose @18:20:00.42 Forearm.reconfigure :tight @18:20:00.46 Forearm.seek CC @18:20:01.69 CC.seek :closed @18:20:06.24 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.28 @18:20:06.31 Hand.open @18:20:06.95 Forearm.reconfigure :loose @18:20:06.99 Forearm.seek :retract @18:20:07.95 PSV.dial PSR @18:20:09.05 Pulling 1.5ml of PSR.flush @18:20:09.06 PSR.select :flush @18:20:09.17 PS.seek 1.5ml @18:20:25.48 delay 5 seconds @18:20:30.50 PSV.dial :waste1 @18:20:30.97 PS.seek 0ml @18:20:47.07 PSV.dial PSR @18:20:47.54 PSR.select :air @18:20:47.65 PS.seek 2.5ml @18:21:13.95 PRV.dial CBV,avoiding: :ab2 @18:21:13.97
CBV.dial PRV @18:21:15.57 PSV.dial :mixloop @18:21:16.60 CTV.dial :air,avoiding: :intake @18:21:16.62 PTV.dial :PRVmixing @18:21:17.38 PS.seek 0ml @18:21:43.73 PSV.dial :air @18:21:44.19 PS.seek 2.5ml @18:22:10.47 PSV.dial :mixloop @18:22:10.95 PS.seek 0ml @18:22:37.35 PSV.dial :air @18:22:37.81 PS.seek 2.5ml @18:23:04.19 PSV.dial :mixloop @18:23:04.65 PS.seek 0ml @18:23:31.23 Garaging FlushPuck in Collection Clamp @18:23:31.75 Elbow.reconfigure :tight @18:23:31.80 Elbow.seek CC @18:23:32.16 Elbow.reconfigure :loose @18:23:32.24 Forearm.seek CC @18:23:33.24 Hand.close @18:23:33.89 CC.seek :open @18:23:38.49 Forearm.seek :retract @18:23:39.48 Elbow.reconfigure :tight @18:23:39.52 Elbow.seek :garage @18:23:42.50 Elbow.reconfigure :loose @18:23:42.59 Forearm.reconfigure :tight @18:23:42.63 Forearm.seek :garage @18:23:43.63 Hand.open @18:23:44.32 Forearm.reconfigure :loose @18:23:44.36 Forearm.seek :retract @18:23:45.33 delayUntil "18:27:52.99" @18:23:45.35 Whole Cell Archive sampling 1000ml @18:23:45.37 Loading wcr puck from tube 2 into Collection Clamp @18:23:45.80 Elbow.reconfigure :tight @18:23:45.85 Elbow.seek 2 @18:23:47.76 Forearm.reconfigure :tight @18:23:47.81 Forearm.seek 2 + 450 counts @18:23:48.86 SE.reconfigure :pick @18:23:48.91 SE.seek :beamBreak @18:23:50.44 SE.configure :default @18:23:50.59 Elbow.seek 2 @18:23:50.76 Forearm.seek 2 + 300 counts @18:23:50.90 Forearm.reconfigure :loose @18:23:50.95 Hand.close @18:23:51.52 SE.seek :down @18:23:51.70 Forearm.seek :retract @18:23:52.61 SE.stop @18:23:52.68 Elbow.seek CC @18:23:54.35 Elbow.reconfigure :loose @18:23:54.45 Forearm.reconfigure :tight @18:23:54.49 Forearm.seek CC @18:23:55.68 CC.seek :closed @18:24:00.19 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.23 @18:24:00.21 Hand.open @18:24:00.82 Forearm.reconfigure :loose @18:24:00.86 Forearm.seek :retract @18:24:01.82 collectSample 1000ml w/bubble point of 35psi @18:24:01.84 Priming 7ml Intake.select :close @18:24:01.85 Exhaust.select :close @18:24:01.86 SS.hold @18:24:02.06 Calibrating pressure sensors @18:24:02.07 CTV.dial :air @18:24:02.08 SSV.dial :spit,avoiding: :exhaust @18:24:02.10 SS.reconfigure :fast @18:24:02.14 SS.seek 5ml @18:24:04.81 SSV.dial CBV @18:24:04.83 CBV.dial SSV @18:24:06.17 delay 3.8 seconds @18:24:10.09 SSV.dial :spit @18:24:11.42 SS.seek 0ml @18:24:14.45 SSV.dial CBV @18:24:15.76 CTV.dial :intake @18:24:16.52 SSV.dial CBV @18:24:16.54 CBV.dial SSV @18:24:17.04 Allowing 40s for 7.0ml of remaining 7.0ml @18:24:17.05 Intake.select :open @18:24:19.20 Intake @15.6psia, vacuum from 10.0 to 13.0psi @18:24:19.22 SS.reconfigure :inhaling 2.20ml/s @18:24:19.26 SS.seek 7ml @18:24:24.79 Stroke completed -- waiting for pressure to equalize ... @18:24:24.80 SS.reconfigure :exhale @18:24:24.84 SS.hold @18:24:33.29 Intake.select :close @18:24:33.44 SSV.dial :exhaust @18:24:34.18 Exhaust.select :open @18:24:34.29 SS.seek 0ml @18:24:38.18 Exhaust.select :close @18:24:38.29 Sampling 1000.0ml @18:24:38.30 Intake.select :close @18:24:38.31 Exhaust.select :close @18:24:38.32 SS.reconfigure :default @18:24:38.36 SS.hold @18:24:38.56 CTV.dial :intake @18:24:38.58 SSV.dial CBV @18:24:38.59 CBV.dial SSV @18:24:39.37 Allowing 130s for 25.0ml of remaining 1000.0ml @18:24:39.38 Intake.select :open @18:24:41.54 Intake @16.0psia, vacuum from 10.0 to 13.0psi @18:24:41.56 SS.reconfigure :inhaling 2.20ml/s @18:24:41.60 SS.seek 25ml @18:24:48.11 Vacuum too high -- waiting up to 40 seconds @18:24:48.12 SS.reconfigure :exhale @18:24:48.17 SS.hold @18:24:49.75 Intake @13.5psia, vacuum from 8.0 to 11.0psi @18:24:49.77 SS.reconfigure :inhaling 1.98ml/s @18:24:49.81 SS.seek 25ml @18:24:56.64 Vacuum too high -- waiting up to 40 seconds @18:24:56.65 SS.reconfigure :exhale @18:24:56.70 SS.hold @18:25:02.61 Intake @14.1psia, vacuum from 8.6 to 11.6psi @18:25:02.63 SS.reconfigure :inhaling 1.79ml/s @18:25:02.67 SS.seek 25ml @18:25:06.93 Stroke completed -- waiting for pressure to equalize ... @18:25:06.95 SS.reconfigure :exhale @18:25:06.99 SS.hold @18:25:10.43 Intake.select :close @18:25:10.57 SSV.dial :exhaust @18:25:11.34 Exhaust.select :open @18:25:11.45 SS.seek 0ml @18:25:20.35 Exhaust.select :close @18:25:20.46 SSV.dial CBV @18:25:20.48 CBV.dial SSV @18:25:21.27 Allowing 130s for 25.0ml of remaining 975.0ml @18:25:21.28 Intake.select :open @18:25:23.43 Intake @15.9psia, vacuum from 10.0 to 13.0psi @18:25:23.45 SS.reconfigure :inhaling 1.79ml/s @18:25:23.49 SS.seek 25ml @18:25:35.99 Vacuum too high -- waiting up to 40 seconds @18:25:36.00 SS.reconfigure :exhale @18:25:36.04 SS.hold @18:25:36.61 Intake @12.3psia, vacuum from 6.8 to 9.8psi @18:25:36.63 SS.reconfigure :inhaling 1.61ml/s @18:25:36.67 SS.seek 25ml @18:25:42.18 Stroke completed -- waiting for pressure to equalize ... @18:25:42.19 SS.reconfigure :exhale @18:25:42.23 SS.hold @18:25:47.76 Intake.select :close @18:25:47.90 SSV.dial :exhaust @18:25:48.66 Exhaust.select :open @18:25:48.78 SS.seek 0ml @18:25:57.49 Exhaust.select :close @18:25:57.60 SSV.dial CBV @18:25:57.62 CBV.dial SSV @18:25:58.40 Allowing 130s for 25.0ml of remaining 950.0ml @18:25:58.41 Intake.select :open @18:26:00.57 Intake @15.6psia, vacuum from 10.0 to 13.0psi @18:26:00.59 SS.reconfigure :inhaling 1.61ml/s @18:26:00.63 SS.seek 25ml @18:26:19.20 Vacuum too high -- waiting up to 40 seconds @18:26:19.21 SS.reconfigure :exhale @18:26:19.26 SS.hold @18:26:19.65 Intake @12.0psia, vacuum from 6.5 to 9.5psi @18:26:19.67 SS.reconfigure :inhaling 1.45ml/s @18:26:19.71 SS.seek 25ml @18:26:20.49 Stroke completed -- waiting for pressure to equalize ... @18:26:20.50 SS.reconfigure :exhale @18:26:20.54 SS.hold @18:26:26.18 Intake.select :close @18:26:26.32 SSV.dial :exhaust @18:26:27.09 Exhaust.select :open @18:26:27.20 SS.seek 0ml @18:26:35.97 Exhaust.select :close @18:26:36.08 SSV.dial CBV @18:26:36.10 CBV.dial SSV @18:26:36.87 Allowing 130s for 25.0ml of remaining 925.0ml @18:26:36.88 Intake.select :open @18:26:39.03 Intake @15.5psia, vacuum from 10.0 to 13.0psi @18:26:39.05 SS.reconfigure :inhaling 1.45ml/s @18:26:39.10 SS.seek 25ml @18:26:59.41 Stroke completed -- waiting for pressure to equalize ... @18:26:59.42 SS.reconfigure :exhale @18:26:59.46 SS.hold @18:27:05.13 Intake.select :close @18:27:05.27 SSV.dial :exhaust @18:27:06.04 Exhaust.select :open @18:27:06.15 SS.seek 0ml @18:27:15.41 Exhaust.select :close @18:27:15.52 SSV.dial CBV @18:27:15.54 CBV.dial SSV @18:27:16.32 Allowing 130s for 25.0ml of remaining 900.0ml @18:27:16.33 Intake.select :open @18:27:18.49 Intake @15.4psia, vacuum from 9.9 to 12.9psi @18:27:18.51 SS.reconfigure :inhaling 1.45ml/s @18:27:18.55 SS.seek 25ml @18:27:38.74 Stroke completed -- waiting for pressure to equalize ... @18:27:38.75 SS.reconfigure :exhale @18:27:38.79 SS.hold @18:27:45.29 Intake.select :close @18:27:45.43 SSV.dial :exhaust @18:27:46.19 Exhaust.select :open @18:27:46.79 SS.seek 0ml @18:27:53.00 recoverPRVreagent08 @18:27:53.01 PTV.dial :mixing @18:27:53.77 PBV.dial :waste1 @18:27:54.13 delay 30 seconds @18:27:55.60 Exhaust.select :close @18:27:55.71 SSV.dial CBV @18:27:55.74 CBV.dial SSV @18:27:56.49 Allowing 130s for 25.0ml of remaining 875.0ml @18:27:56.50 Intake.select :open @18:27:58.66 Intake @15.5psia, vacuum from 10.0 to 13.0psi @18:27:58.68 SS.reconfigure :inhaling 1.45ml/s @18:27:58.72 SS.seek 25ml @18:28:16.17 Vacuum too high -- waiting up to 40 seconds @18:28:16.18 SS.reconfigure :exhale @18:28:16.22 SS.hold @18:28:16.71 Intake @12.1psia, vacuum from 6.6 to 9.6psi @18:28:16.73 SS.reconfigure :inhaling 1.30ml/s @18:28:16.77 SS.seek 25ml @18:28:20.41 Stroke completed -- waiting for pressure to equalize ... @18:28:20.42 SS.reconfigure :exhale @18:28:20.46 SS.hold @18:28:24.14 PBV.dial between PSV and waste1 @18:28:24.56 PSV.dial :air @18:28:25.16 PS.reconfigure :fast @18:28:25.21 PS.seek 2.5ml @18:28:26.19 Intake.select :close @18:28:26.33 SSV.dial :exhaust @18:28:27.10 Exhaust.select :open @18:28:27.21 SS.seek 0ml @18:28:36.21 Exhaust.select :close @18:28:36.32 SSV.dial CBV @18:28:36.33 CBV.dial SSV @18:28:37.10 Allowing 130s for 25.0ml of remaining 850.0ml @18:28:37.11 Intake.select :open @18:28:39.27 Intake @15.4psia, vacuum from 9.9 to 12.9psi @18:28:39.29 SS.reconfigure :inhaling 1.30ml/s @18:28:39.33 SS.seek 25ml @18:28:49.31 PSV.dial :mixloop @18:28:49.77 delay 2 seconds @18:28:51.78 PSV.dial :air @18:28:52.38 delay 2 seconds @18:28:54.39 PSV.dial :mixloop @18:28:54.85 delay 2 seconds @18:28:56.86 PSV.dial :air @18:28:57.34 PS.seek 0ml @18:29:01.28 Stroke completed -- waiting for pressure to equalize ... @18:29:01.30 SS.reconfigure :exhale @18:29:01.34 SS.hold @18:29:08.70 Intake.select :close @18:29:08.84 SSV.dial :exhaust @18:29:09.60 Exhaust.select :open @18:29:09.71 SS.seek 0ml @18:29:18.46 Exhaust.select :close @18:29:18.56 SSV.dial CBV @18:29:18.59 CBV.dial SSV @18:29:19.35 Allowing 130s for 25.0ml of remaining 825.0ml @18:29:19.36 Intake.select :open @18:29:20.81 washCycleDA #1 of 2 @18:29:20.82 PSR.select :air @18:29:20.84 PSV.dial PSR @18:29:21.51 Intake @15.4psia, vacuum from 9.9 to 12.9psi @18:29:21.53 SS.reconfigure :inhaling 1.30ml/s @18:29:21.57 SS.seek 25ml @18:29:22.21 Pulling 0.5ml of PSR.wash @18:29:22.22 PSR.select :wash @18:29:22.33 PS.reconfigure :default @18:29:22.38 PS.seek 0.5ml @18:29:28.35 delay 5 seconds @18:29:33.36 PSR.select :air @18:29:33.48 PS.seek 2ml @18:29:42.70 Vacuum too high -- waiting up to 40 seconds @18:29:42.71 SS.reconfigure :exhale @18:29:42.75 SS.hold @18:29:43.22 Intake @12.4psia, vacuum from 6.9 to 9.9psi @18:29:43.23 SS.reconfigure :inhaling 1.17ml/s @18:29:43.28 SS.seek 25ml @18:29:43.83 Vacuum too high -- waiting up to 40 seconds @18:29:43.84 SS.reconfigure :exhale @18:29:43.88 SS.hold @18:29:48.76 Intake @13.5psia, vacuum from 8.0 to 11.0psi @18:29:48.78 SS.reconfigure :inhaling 1.06ml/s @18:29:48.82 SS.seek 25ml @18:29:49.59 PTV.dial :puck @18:29:50.34 PSV.dial PTV @18:29:50.44 Stroke completed -- waiting for pressure to equalize ... @18:29:50.45 SS.reconfigure :exhale @18:29:50.49 SS.hold @18:29:51.25 PBV.dial :waste1 @18:29:51.42 Intake.select :close @18:29:51.57 SSV.dial :exhaust @18:29:51.69 PS.seek 1.5ml @18:29:52.33 Exhaust.select :open @18:29:52.44 SS.seek 0ml @18:29:57.56 delay 3 seconds @18:30:00.58 PBV.dial between PSV and waste1 @18:30:00.96 PS.seek 0ml @18:30:01.45 Exhaust.select :close @18:30:01.56 SSV.dial CBV @18:30:01.59 CBV.dial SSV @18:30:02.35 Allowing 130s for 25.0ml of remaining 800.0ml @18:30:02.36 Intake.select :open @18:30:04.52 Intake @15.4psia, vacuum from 9.9 to 12.9psi @18:30:04.53 SS.reconfigure :inhaling 1.06ml/s @18:30:04.58 SS.seek 25ml @18:30:17.09 delay 2:00 @18:30:30.61 Stroke completed -- waiting for pressure to equalize ... @18:30:30.62 SS.reconfigure :exhale @18:30:30.66 SS.hold @18:30:36.31 Intake.select :close @18:30:36.45 SSV.dial :exhaust @18:30:37.21 Exhaust.select :open @18:30:37.32 SS.seek 0ml @18:30:46.44 Exhaust.select :close @18:30:46.55 SSV.dial CBV @18:30:46.56 CBV.dial SSV @18:30:47.32 Allowing 130s for 25.0ml of remaining 775.0ml @18:30:47.33 Intake.select :open @18:30:49.48 Intake @15.3psia, vacuum from 9.8 to 12.8psi @18:30:49.50 SS.reconfigure :inhaling 1.06ml/s @18:30:49.54 SS.seek 25ml @18:31:15.41 Stroke completed -- waiting for pressure to equalize ... @18:31:15.42 SS.reconfigure :exhale @18:31:15.46 SS.hold @18:31:21.98 Intake.select :close @18:31:22.14 SSV.dial :exhaust @18:31:22.89 Exhaust.select :open @18:31:23.00 SS.seek 0ml @18:31:31.81 Exhaust.select :close @18:31:31.92 SSV.dial CBV @18:31:31.94 CBV.dial SSV @18:31:32.71 Allowing 130s for 25.0ml of remaining 750.0ml @18:31:32.72 Intake.select :open @18:31:34.87 Intake @15.3psia, vacuum from 9.8 to 12.8psi @18:31:34.89 SS.reconfigure :inhaling 1.06ml/s @18:31:34.93 SS.seek 25ml @18:32:01.45 Stroke completed -- waiting for pressure to equalize ... @18:32:01.46 SS.reconfigure :exhale @18:32:01.51 SS.hold @18:32:08.53 Intake.select :close @18:32:08.67 SSV.dial :exhaust @18:32:09.42 Exhaust.select :open @18:32:09.53 SS.seek 0ml @18:32:17.12 recoverPSRreagent08 @18:32:17.13 PTV.dial :puck @18:32:17.14 PBV.dial :waste1 @18:32:17.50 delay 30 seconds @18:32:18.53 Exhaust.select :close @18:32:18.64 SSV.dial CBV @18:32:18.66 CBV.dial SSV @18:32:19.42 Allowing 130s for 25.0ml of remaining 725.0ml @18:32:19.44 Intake.select :open @18:32:21.59 Intake @15.4psia, vacuum from 9.9 to 12.9psi @18:32:21.61 SS.reconfigure :inhaling 1.06ml/s @18:32:21.65 SS.seek 25ml @18:32:47.52 PBV.dial between PSV and waste1 @18:32:47.75 Stroke completed -- waiting for pressure to equalize ... @18:32:47.76 SS.reconfigure :exhale @18:32:47.80 SS.hold @18:32:47.89 PSR.select :air @18:32:47.91 PSV.dial PSR @18:32:48.69 PS.reconfigure :fast @18:32:48.79 PS.seek 2.5ml @18:32:56.41 Intake.select :close @18:32:56.56 SSV.dial :exhaust @18:32:57.31 Exhaust.select :open @18:32:57.42 SS.seek 0ml @18:33:06.09 Exhaust.select :close @18:33:06.20 SSV.dial CBV @18:33:06.22 CBV.dial SSV @18:33:07.00 Allowing 130s for 25.0ml of remaining 700.0ml @18:33:07.01 Intake.select :open @18:33:09.17 Intake @15.4psia, vacuum from 9.9 to 12.9psi @18:33:09.19 SS.reconfigure :inhaling 1.06ml/s @18:33:09.23 SS.seek 25ml @18:33:12.90 PSV.dial PTV @18:33:13.67 delay 5 seconds @18:33:18.69 PSV.dial PSR @18:33:19.43 delay 5 seconds @18:33:24.45 PSV.dial PTV @18:33:25.22 delay 5 seconds @18:33:30.24 PSV.dial :air @18:33:31.00 PS.seek 0ml @18:33:33.88 Vacuum too high -- waiting up to 40 seconds @18:33:33.89 SS.reconfigure :exhale @18:33:33.94 SS.hold @18:33:36.99 Intake @13.3psia, vacuum from 7.8 to 10.8psi @18:33:37.01 SS.reconfigure :inhaling 0.95ml/s @18:33:37.05 SS.seek 25ml @18:33:39.51 Stroke completed -- waiting for pressure to equalize ... @18:33:39.52 SS.reconfigure :exhale @18:33:39.57 SS.hold @18:33:45.10 Intake.select :close @18:33:45.24 SSV.dial :exhaust @18:33:46.01 Exhaust.select :open @18:33:46.11 SS.seek 0ml @18:33:54.53 washCycleDA #2 of 2 @18:33:54.54 PSR.select :air @18:33:54.55 PSV.dial PSR @18:33:55.40 Exhaust.select :close @18:33:55.51 SSV.dial CBV @18:33:55.53 CBV.dial SSV @18:33:55.92 Pulling 0.5ml of PSR.wash @18:33:55.94 PSR.select :wash @18:33:56.05 PS.reconfigure :default @18:33:56.09 PS.seek 0.5ml @18:33:56.31 Allowing 130s for 25.0ml of remaining 675.0ml @18:33:56.33 Intake.select :open @18:33:58.48 Intake @15.4psia, vacuum from 9.9 to 12.9psi @18:33:58.50 SS.reconfigure :inhaling 0.95ml/s @18:33:58.55 SS.seek 25ml @18:34:02.09 delay 5 seconds @18:34:07.11 PSR.select :air @18:34:07.22 PS.seek 2ml @18:34:23.34 PTV.dial :puck @18:34:23.35 PSV.dial PTV @18:34:24.09 PBV.dial :waste1 @18:34:24.52 PS.seek 1.5ml @18:34:27.75 Stroke completed -- waiting for pressure to equalize ... @18:34:27.76 SS.reconfigure :exhale @18:34:27.80 SS.hold @18:34:30.43 delay 3 seconds @18:34:33.45 PBV.dial between PSV and waste1 @18:34:33.83 PS.seek 0ml @18:34:36.31 Intake.select :close @18:34:36.45 SSV.dial :exhaust @18:34:37.20 Exhaust.select :open @18:34:37.31 SS.seek 0ml @18:34:45.98 Exhaust.select :close @18:34:46.09 SSV.dial CBV @18:34:46.11 CBV.dial SSV @18:34:46.89 Allowing 130s for 25.0ml of remaining 650.0ml @18:34:46.90 Intake.select :open @18:34:49.06 Intake @15.4psia, vacuum from 9.9 to 12.9psi @18:34:49.07 SS.reconfigure :inhaling 0.95ml/s @18:34:49.12 SS.seek 25ml @18:34:50.00 delay 2:00 @18:35:18.13 Vacuum too high -- waiting up to 40 seconds @18:35:18.14 SS.reconfigure :exhale @18:35:18.18 SS.hold @18:35:21.73 Intake @13.5psia, vacuum from 8.0 to 11.0psi @18:35:21.75 SS.reconfigure :inhaling 0.87ml/s @18:35:21.79 SS.seek 25ml @18:35:22.15 Stroke completed -- waiting for pressure to equalize ... @18:35:22.16 SS.reconfigure :exhale @18:35:22.20 SS.hold @18:35:27.84 Intake.select :close @18:35:27.98 SSV.dial :exhaust @18:35:28.75 Exhaust.select :open @18:35:28.86 SS.seek 0ml @18:35:37.87 Exhaust.select :close @18:35:37.98 SSV.dial CBV @18:35:38.00 CBV.dial SSV @18:35:38.76 Allowing 130s for 25.0ml of remaining 625.0ml @18:35:38.78 Intake.select :open @18:35:40.93 Intake @15.4psia, vacuum from 9.9 to 12.9psi @18:35:40.95 SS.reconfigure :inhaling 0.87ml/s @18:35:40.99 SS.seek 25ml @18:36:12.17 Stroke completed -- waiting for pressure to equalize ... @18:36:12.18 SS.reconfigure :exhale @18:36:12.22 SS.hold @18:36:21.25 Intake.select :close @18:36:21.39 SSV.dial :exhaust @18:36:22.14 Exhaust.select :open @18:36:22.25 SS.seek 0ml @18:36:30.96 Exhaust.select :close @18:36:31.07 SSV.dial CBV @18:36:31.09 CBV.dial SSV @18:36:31.86 Allowing 130s for 25.0ml of remaining 600.0ml @18:36:31.87 Intake.select :open @18:36:34.02 Intake @15.4psia, vacuum from 9.9 to 12.9psi @18:36:34.04 SS.reconfigure :inhaling 0.87ml/s @18:36:34.08 SS.seek 25ml @18:36:50.01 recoverPSRreagent08 @18:36:50.03 PTV.dial :puck @18:36:50.04 PBV.dial :waste1 @18:36:50.40 delay 30 seconds @18:37:05.44 Vacuum too high -- waiting up to 40 seconds @18:37:05.45 SS.reconfigure :exhale @18:37:05.50 SS.hold @18:37:09.67 Intake @13.8psia, vacuum from 8.3 to 11.3psi @18:37:09.69 SS.reconfigure :inhaling 0.78ml/s @18:37:09.73 SS.seek 25ml @18:37:10.55 Stroke completed -- waiting for pressure to equalize ... @18:37:10.56 SS.reconfigure :exhale @18:37:10.60 SS.hold @18:37:16.13 Intake.select :close @18:37:16.27 SSV.dial :exhaust @18:37:17.03 Exhaust.select :open @18:37:17.14 SS.seek 0ml @18:37:20.41 PBV.dial between PSV and waste1 @18:37:20.79 PSR.select :air @18:37:20.81 PSV.dial PSR @18:37:21.57 PS.reconfigure :fast @18:37:21.62 PS.seek 2.5ml @18:37:26.04 Exhaust.select :close @18:37:26.15 SSV.dial CBV @18:37:26.17 CBV.dial SSV @18:37:26.94 Allowing 130s for 25.0ml of remaining 575.0ml @18:37:26.96 Intake.select :open @18:37:29.11 Intake @15.4psia, vacuum from 9.9 to 12.9psi @18:37:29.13 SS.reconfigure :inhaling 0.78ml/s @18:37:29.17 SS.seek 25ml @18:37:45.68 PSV.dial PTV @18:37:46.45 delay 5 seconds @18:37:51.46 PSV.dial PSR @18:37:52.23 delay 5 seconds @18:37:57.25 PSV.dial PTV @18:37:58.01 delay 5 seconds @18:38:03.03 PSV.dial :air @18:38:03.79 PS.seek 0ml @18:38:04.12 Stroke completed -- waiting for pressure to equalize ... @18:38:04.13 SS.reconfigure :exhale @18:38:04.17 SS.hold @18:38:13.95 Intake.select :close @18:38:14.09 SSV.dial :exhaust @18:38:15.34 Exhaust.select :open @18:38:15.45 SS.seek 0ml @18:38:24.29 Exhaust.select :close @18:38:24.40 SSV.dial CBV @18:38:24.44 CBV.dial SSV @18:38:25.17 Allowing 130s for 25.0ml of remaining 550.0ml @18:38:25.18 Intake.select :open @18:38:27.24 getPMLReagent08 @18:38:27.25 PRV.dial :air @18:38:27.35 Intake @15.4psia, vacuum from 9.9 to 12.9psi @18:38:27.37 SS.reconfigure :inhaling 0.78ml/s @18:38:27.41 SS.seek 25ml @18:38:27.71 delay 5 seconds @18:38:32.73 PTV.dial :PRVmixing @18:38:34.05 delay 5 seconds @18:38:39.07 PRV.dial :ab2 @18:38:39.54 PTV.dial :PRVmixing @18:38:39.55 PSV.dial :mixloop @18:38:40.01 PS.reconfigure :default @18:38:40.05 PS.seek 0.3ml @18:38:43.99 delay 2 seconds @18:38:46.01 PRV.dial :air @18:38:46.53 PS.seek 0.55ml @18:38:49.93 addPMLReagent08 @18:38:49.94 PSV.dial :air @18:38:50.40 PS.seek 2ml @18:39:02.40 Stroke completed -- waiting for pressure to equalize ... @18:39:02.41 SS.reconfigure :exhale @18:39:02.45 SS.hold @18:39:06.08 PSV.dial :PML @18:39:06.59 PTV.dial :mixing @18:39:07.33 PBV.dial :waste1 @18:39:07.75 PS.seek 1.85ml @18:39:10.05 PBV.dial between PSV and waste1 @18:39:10.43 PS.seek 0ml @18:39:13.58 Intake.select :close @18:39:13.72 SSV.dial :exhaust @18:39:14.48 Exhaust.select :open @18:39:14.59 SS.seek 0ml @18:39:23.63 Exhaust.select :close @18:39:23.74 SSV.dial CBV @18:39:23.76 CBV.dial SSV @18:39:24.52 Allowing 130s for 25.0ml of remaining 525.0ml @18:39:24.54 Intake.select :open @18:39:26.69 Intake @15.5psia, vacuum from 10.0 to 13.0psi @18:39:26.71 SS.reconfigure :inhaling 0.78ml/s @18:39:26.75 SS.seek 25ml @18:39:30.33 delay 2 seconds @18:39:32.35 delay 8:00 @18:39:58.28 Vacuum too high -- waiting up to 40 seconds @18:39:58.29 SS.reconfigure :exhale @18:39:58.33 SS.hold @18:40:03.99 Intake @14.1psia, vacuum from 8.6 to 11.6psi @18:40:04.01 SS.reconfigure :inhaling 0.70ml/s @18:40:04.05 SS.seek 25ml @18:40:07.80 Stroke completed -- waiting for pressure to equalize ... @18:40:07.81 SS.reconfigure :exhale @18:40:07.85 SS.hold @18:40:14.36 Intake.select :close @18:40:14.50 SSV.dial :exhaust @18:40:15.24 Exhaust.select :open @18:40:15.35 SS.seek 0ml @18:40:24.50 Exhaust.select :close @18:40:24.61 SSV.dial CBV @18:40:24.63 CBV.dial SSV @18:40:25.38 Allowing 130s for 25.0ml of remaining 500.0ml @18:40:25.40 Intake.select :open @18:40:27.55 Intake @15.4psia, vacuum from 9.9 to 12.9psi @18:40:27.57 SS.reconfigure :inhaling 0.70ml/s @18:40:27.61 SS.seek 25ml @18:41:05.99 Stroke completed -- waiting for pressure to equalize ... @18:41:06.00 SS.reconfigure :exhale @18:41:06.04 SS.hold @18:41:18.61 Intake.select :close @18:41:18.75 SSV.dial :exhaust @18:41:19.49 Exhaust.select :open @18:41:19.60 SS.seek 0ml @18:41:28.33 Exhaust.select :close @18:41:28.44 SSV.dial CBV @18:41:28.46 CBV.dial SSV @18:41:29.23 Allowing 130s for 25.0ml of remaining 475.0ml @18:41:29.24 Intake.select :open @18:41:31.39 Intake @15.4psia, vacuum from 9.9 to 12.9psi @18:41:31.41 SS.reconfigure :inhaling 0.70ml/s @18:41:31.45 SS.seek 25ml @18:42:03.12 Vacuum too high -- waiting up to 40 seconds @18:42:03.13 SS.reconfigure :exhale @18:42:03.18 SS.hold @18:42:08.81 Intake @14.3psia, vacuum from 8.8 to 11.8psi @18:42:08.83 SS.reconfigure :inhaling 0.63ml/s @18:42:08.87 SS.seek 25ml @18:42:16.39 Stroke completed -- waiting for pressure to equalize ... @18:42:16.40 SS.reconfigure :exhale @18:42:16.45 SS.hold @18:42:24.90 Intake.select :close @18:42:25.04 SSV.dial :exhaust @18:42:25.79 Exhaust.select :open @18:42:25.89 SS.seek 0ml @18:42:34.69 Exhaust.select :close @18:42:34.80 SSV.dial CBV @18:42:34.82 CBV.dial SSV @18:42:35.58 Allowing 130s for 25.0ml of remaining 450.0ml @18:42:35.60 Intake.select :open @18:42:37.75 Intake @15.4psia, vacuum from 9.9 to 12.9psi @18:42:37.77 SS.reconfigure :inhaling 0.63ml/s @18:42:37.81 SS.seek 25ml @18:43:20.06 Stroke completed -- waiting for pressure to equalize ... @18:43:20.07 SS.reconfigure :exhale @18:43:20.12 SS.hold @18:43:34.38 Intake.select :close @18:43:34.52 SSV.dial :exhaust @18:43:35.27 Exhaust.select :open @18:43:35.38 SS.seek 0ml @18:43:44.20 Exhaust.select :close @18:43:44.31 SSV.dial CBV @18:43:44.33 CBV.dial SSV @18:43:45.10 Allowing 130s for 25.0ml of remaining 425.0ml @18:43:45.11 Intake.select :open @18:43:47.26 Intake @15.4psia, vacuum from 9.9 to 12.9psi @18:43:47.28 SS.reconfigure :inhaling 0.63ml/s @18:43:47.33 SS.seek 25ml @18:44:20.74 Vacuum too high -- waiting up to 40 seconds @18:44:20.76 SS.reconfigure :exhale @18:44:20.80 SS.hold @18:44:26.44 Intake @14.5psia, vacuum from 9.0 to 12.0psi @18:44:26.46 SS.reconfigure :inhaling 0.57ml/s @18:44:26.50 SS.seek 25ml @18:44:36.55 Stroke completed -- waiting for pressure to equalize ... @18:44:36.56 SS.reconfigure :exhale @18:44:36.60 SS.hold @18:44:46.41 Intake.select :close @18:44:46.55 SSV.dial :exhaust @18:44:47.30 Exhaust.select :open @18:44:47.41 SS.seek 0ml @18:44:56.51 Exhaust.select :close @18:44:56.62 SSV.dial CBV @18:44:56.65 CBV.dial SSV @18:44:57.39 Allowing 130s for 25.0ml of remaining 400.0ml @18:44:57.41 Intake.select :open @18:44:59.56 Intake @15.5psia, vacuum from 10.0 to 13.0psi @18:44:59.58 SS.reconfigure :inhaling 0.57ml/s @18:44:59.62 SS.seek 25ml @18:45:45.31 Stroke completed -- waiting for pressure to equalize ... @18:45:45.32 SS.reconfigure :exhale @18:45:45.36 SS.hold @18:46:01.64 Intake.select :close @18:46:01.79 SSV.dial :exhaust @18:46:02.53 Exhaust.select :open @18:46:02.64 SS.seek 0ml @18:46:11.73 Exhaust.select :close @18:46:11.84 SSV.dial CBV @18:46:11.86 CBV.dial SSV @18:46:12.81 Allowing 130s for 25.0ml of remaining 375.0ml @18:46:12.83 Intake.select :open @18:46:14.98 Intake @15.5psia, vacuum from 10.0 to 13.0psi @18:46:15.00 SS.reconfigure :inhaling 0.57ml/s @18:46:15.04 SS.seek 25ml @18:46:52.80 Vacuum too high -- waiting up to 40 seconds @18:46:53.23 SS.reconfigure :exhale @18:46:53.36 SS.hold @18:46:59.71 Intake @14.7psia, vacuum from 9.2 to 12.2psi @18:46:59.73 SS.reconfigure :inhaling 0.51ml/s @18:46:59.77 SS.seek 25ml @18:47:09.40 Stroke completed -- waiting for pressure to equalize ... @18:47:09.41 SS.reconfigure :exhale @18:47:09.46 SS.hold @18:47:20.52 Intake.select :close @18:47:20.66 SSV.dial :exhaust @18:47:21.40 Exhaust.select :open @18:47:21.51 SS.seek 0ml @18:47:30.53 Exhaust.select :close @18:47:30.63 SSV.dial CBV @18:47:30.65 CBV.dial SSV @18:47:31.41 Allowing 130s for 25.0ml of remaining 350.0ml @18:47:31.42 Intake.select :open @18:47:32.37 recoverPRVreagent08 @18:47:32.38 PTV.dial :mixing @18:47:32.39 PBV.dial :waste1 @18:47:32.75 delay 30 seconds @18:47:33.57 Intake @15.5psia, vacuum from 10.0 to 13.0psi @18:47:33.59 SS.reconfigure :inhaling 0.51ml/s @18:47:33.63 SS.seek 25ml @18:48:02.77 PBV.dial between PSV and waste1 @18:48:03.12 PSV.dial :air @18:48:03.73 PS.reconfigure :fast @18:48:03.78 PS.seek 2.5ml @18:48:24.57 Stroke completed -- waiting for pressure to equalize ... @18:48:24.59 SS.reconfigure :exhale @18:48:24.63 SS.hold @18:48:27.98 PSV.dial :mixloop @18:48:28.42 delay 2 seconds @18:48:30.43 PSV.dial :air @18:48:30.90 delay 2 seconds @18:48:32.92 PSV.dial :mixloop @18:48:33.38 delay 2 seconds @18:48:35.40 PSV.dial :air @18:48:35.87 PS.seek 0ml @18:48:42.98 Intake.select :close @18:48:43.13 SSV.dial :exhaust @18:48:43.87 Exhaust.select :open @18:48:43.98 SS.seek 0ml @18:48:52.70 Exhaust.select :close @18:48:52.81 SSV.dial CBV @18:48:52.84 CBV.dial SSV @18:48:53.58 Allowing 130s for 25.0ml of remaining 325.0ml @18:48:53.59 Intake.select :open @18:48:55.74 Intake @15.4psia, vacuum from 9.9 to 12.9psi @18:48:55.76 SS.reconfigure :inhaling 0.51ml/s @18:48:55.80 SS.seek 25ml @18:48:59.54 washCycleDA #1 of 3 @18:48:59.55 PSR.select :air @18:48:59.57 PSV.dial PSR @18:49:00.94 Pulling 0.5ml of PSR.wash @18:49:00.95 PSR.select :wash @18:49:01.42 PS.reconfigure :default @18:49:01.55 PS.seek 0.5ml @18:49:07.45 delay 5 seconds @18:49:12.46 PSR.select :air @18:49:12.58 PS.seek 2ml @18:49:28.69 PTV.dial :puck @18:49:29.45 PSV.dial PTV @18:49:30.20 PBV.dial :waste1 @18:49:30.63 PS.seek 1.5ml @18:49:36.51 delay 3 seconds @18:49:38.49 Vacuum too high -- waiting up to 40 seconds @18:49:38.50 SS.reconfigure :exhale @18:49:38.55 SS.hold @18:49:39.52 PBV.dial between PSV and waste1 @18:49:39.91 PS.seek 0ml @18:49:48.33 Intake @14.9psia, vacuum from 9.4 to 12.4psi @18:49:48.35 SS.reconfigure :inhaling 0.47ml/s @18:49:48.39 SS.seek 25ml @18:49:56.10 delay 2:00 @18:49:58.42 Stroke completed -- waiting for pressure to equalize ... @18:49:58.43 SS.reconfigure :exhale @18:49:58.47 SS.hold @18:50:11.07 Intake.select :close @18:50:11.22 SSV.dial :exhaust @18:50:11.97 Exhaust.select :open @18:50:12.08 SS.seek 0ml @18:50:20.87 Exhaust.select :close @18:50:20.97 SSV.dial CBV @18:50:21.03 CBV.dial SSV @18:50:21.73 Allowing 130s for 25.0ml of remaining 300.0ml @18:50:21.74 Intake.select :open @18:50:23.90 Intake @15.6psia, vacuum from 10.0 to 13.0psi @18:50:23.92 SS.reconfigure :inhaling 0.47ml/s @18:50:23.96 SS.seek 25ml @18:51:11.52 Vacuum too high -- waiting up to 40 seconds @18:51:11.53 SS.reconfigure :exhale @18:51:11.57 SS.hold @18:51:20.12 Intake @15.0psia, vacuum from 9.5 to 12.5psi @18:51:20.14 SS.reconfigure :inhaling 0.43ml/s @18:51:20.18 SS.seek 25ml @18:51:30.55 Stroke completed -- waiting for pressure to equalize ... @18:51:30.56 SS.reconfigure :exhale @18:51:30.61 SS.hold @18:51:43.25 Intake.select :close @18:51:43.39 SSV.dial :exhaust @18:51:44.13 Exhaust.select :open @18:51:44.24 SS.seek 0ml @18:51:53.05 Exhaust.select :close @18:51:53.16 SSV.dial CBV @18:51:53.18 CBV.dial SSV @18:51:53.95 Allowing 130s for 25.0ml of remaining 275.0ml @18:51:53.96 Intake.select :open @18:51:56.12 Intake @15.6psia, vacuum from 10.0 to 13.0psi @18:51:56.13 SS.reconfigure :inhaling 0.43ml/s @18:51:56.18 SS.seek 25ml @18:51:56.22 recoverPSRreagent08 @18:51:56.23 PTV.dial :puck @18:51:56.24 PBV.dial :waste1 @18:51:56.62 delay 30 seconds @18:52:26.64 PBV.dial between PSV and waste1 @18:52:27.01 PSR.select :air @18:52:27.02 PSV.dial PSR @18:52:27.79 PS.reconfigure :fast @18:52:27.84 PS.seek 2.5ml @18:52:51.97 PSV.dial PTV @18:52:52.74 delay 5 seconds @18:52:57.76 PSV.dial PSR @18:52:58.52 delay 5 seconds @18:52:59.82 Stroke completed -- waiting for pressure to equalize ... @18:52:59.83 SS.reconfigure :exhale @18:52:59.87 SS.hold @18:53:03.54 PSV.dial PTV @18:53:04.30 delay 5 seconds @18:53:09.32 PSV.dial :air @18:53:10.09 PS.seek 0ml @18:53:20.23 Intake.select :close @18:53:20.37 SSV.dial :exhaust @18:53:21.12 Exhaust.select :open @18:53:21.23 SS.seek 0ml @18:53:30.02 Exhaust.select :close @18:53:30.13 SSV.dial CBV @18:53:30.15 CBV.dial SSV @18:53:30.91 Allowing 130s for 25.0ml of remaining 250.0ml @18:53:30.93 Intake.select :open @18:53:33.08 Intake @15.6psia, vacuum from 10.0 to 13.0psi @18:53:33.10 SS.reconfigure :inhaling 0.43ml/s @18:53:33.14 SS.seek 25ml @18:53:33.63 washCycleDA #2 of 3 @18:53:33.64 PSR.select :air @18:53:33.66 PSV.dial PSR @18:53:35.03 Pulling 0.5ml of PSR.wash @18:53:35.04 PSR.select :wash @18:53:35.16 PS.reconfigure :default @18:53:35.20 PS.seek 0.5ml @18:53:41.07 delay 5 seconds @18:53:46.09 PSR.select :air @18:53:46.20 PS.seek 2ml @18:54:02.38 PTV.dial :puck @18:54:02.39 PSV.dial PTV @18:54:03.13 PBV.dial :waste1 @18:54:03.56 PS.seek 1.5ml @18:54:09.57 delay 3 seconds @18:54:12.58 PBV.dial between PSV and waste1 @18:54:12.96 PS.seek 0ml @18:54:23.01 Vacuum too high -- waiting up to 40 seconds @18:54:23.03 SS.reconfigure :exhale @18:54:23.07 SS.hold @18:54:29.06 delay 2:00 @18:54:32.82 Intake @15.0psia, vacuum from 9.5 to 12.5psi @18:54:32.84 SS.reconfigure :inhaling 0.38ml/s @18:54:32.88 SS.seek 25ml @18:54:47.82 Stroke completed -- waiting for pressure to equalize ... @18:54:47.83 SS.reconfigure :exhale @18:54:47.87 SS.hold @18:55:04.07 Intake.select :close @18:55:04.22 SSV.dial :exhaust @18:55:04.97 Exhaust.select :open @18:55:05.08 SS.seek 0ml @18:55:13.88 Exhaust.select :close @18:55:13.98 SSV.dial CBV @18:55:14.04 CBV.dial SSV @18:55:14.74 Allowing 130s for 25.0ml of remaining 225.0ml @18:55:14.75 Intake.select :open @18:55:16.91 Intake @15.6psia, vacuum from 10.0 to 13.0psi @18:55:16.93 SS.reconfigure :inhaling 0.38ml/s @18:55:16.97 SS.seek 25ml @18:56:29.09 recoverPSRreagent08 @18:56:29.10 PTV.dial :puck @18:56:29.11 PBV.dial :waste1 @18:56:29.49 delay 30 seconds @18:56:29.61 Stroke completed -- waiting for pressure to equalize ... @18:56:29.62 SS.reconfigure :exhale @18:56:29.66 SS.hold @18:56:54.11 Intake.select :close @18:56:54.26 SSV.dial :exhaust @18:56:55.00 Exhaust.select :open @18:56:55.11 SS.seek 0ml @18:56:59.50 PBV.dial between PSV and waste1 @18:56:59.88 PSR.select :air @18:56:59.89 PSV.dial PSR @18:57:00.66 PS.reconfigure :fast @18:57:00.71 PS.seek 2.5ml @18:57:03.81 Exhaust.select :close @18:57:03.92 SSV.dial CBV @18:57:03.94 CBV.dial SSV @18:57:04.69 Allowing 130s for 25.0ml of remaining 200.0ml @18:57:04.70 Intake.select :open @18:57:06.86 Intake @15.6psia, vacuum from 10.0 to 13.0psi @18:57:06.88 SS.reconfigure :inhaling 0.38ml/s @18:57:06.92 SS.seek 25ml @18:57:24.78 PSV.dial PTV @18:57:25.55 delay 5 seconds @18:57:30.57 PSV.dial PSR @18:57:31.33 delay 5 seconds @18:57:36.35 PSV.dial PTV @18:57:37.11 delay 5 seconds @18:57:42.13 PSV.dial :air @18:57:42.90 PS.seek 0ml @18:58:06.31 washCycleDA #3 of 3 @18:58:06.32 PSR.select :air @18:58:06.34 PSV.dial PSR @18:58:07.71 Pulling 0.5ml of PSR.wash @18:58:07.72 PSR.select :wash @18:58:07.83 PS.reconfigure :default @18:58:07.87 PS.seek 0.5ml @18:58:09.35 Vacuum too high -- waiting up to 40 seconds @18:58:09.37 SS.reconfigure :exhale @18:58:09.41 SS.hold @18:58:13.78 delay 5 seconds @18:58:18.84 PSR.select :air @18:58:18.95 PS.seek 2ml @18:58:21.46 Intake @15.2psia, vacuum from 9.7 to 12.7psi @18:58:21.48 SS.reconfigure :inhaling 0.34ml/s @18:58:21.52 SS.seek 25ml @18:58:34.36 Stroke completed -- waiting for pressure to equalize ... @18:58:34.37 SS.reconfigure :exhale @18:58:34.41 SS.hold @18:58:35.09 PTV.dial :puck @18:58:35.10 PSV.dial PTV @18:58:35.84 PBV.dial :waste1 @18:58:36.26 PS.seek 1.5ml @18:58:42.21 delay 3 seconds @18:58:45.23 PBV.dial between PSV and waste1 @18:58:45.61 PS.seek 0ml @18:58:52.72 Intake.select :close @18:58:52.86 SSV.dial :exhaust @18:58:53.61 Exhaust.select :open @18:58:53.72 SS.seek 0ml @18:59:01.75 delay 2:00 @18:59:02.48 Exhaust.select :close @18:59:02.59 SSV.dial CBV @18:59:02.61 CBV.dial SSV @18:59:03.38 Allowing 130s for 25.0ml of remaining 175.0ml @18:59:03.39 Intake.select :open @18:59:05.54 Intake @15.7psia, vacuum from 10.0 to 13.0psi @18:59:05.56 SS.reconfigure :inhaling 0.34ml/s @18:59:05.60 SS.seek 25ml @19:00:32.90 Stroke completed -- waiting for pressure to equalize ... @19:00:32.91 SS.reconfigure :exhale @19:00:32.96 SS.hold @19:00:59.36 Intake.select :close @19:00:59.50 SSV.dial :exhaust @19:01:00.25 Exhaust.select :open @19:01:00.36 SS.seek 0ml @19:01:01.77 recoverPSRreagent08 @19:01:01.78 PTV.dial :puck @19:01:01.79 PBV.dial :waste1 @19:01:02.15 delay 30 seconds @19:01:09.34 Exhaust.select :close @19:01:09.45 SSV.dial CBV @19:01:09.48 CBV.dial SSV @19:01:10.22 Allowing 130s for 25.0ml of remaining 150.0ml @19:01:10.23 Intake.select :open @19:01:12.86 Intake @15.5psia, vacuum from 10.0 to 13.0psi @19:01:12.88 SS.reconfigure :inhaling 0.34ml/s @19:01:12.92 SS.seek 25ml @19:01:32.17 PBV.dial between PSV and waste1 @19:01:32.54 PSR.select :air @19:01:32.56 PSV.dial PSR @19:01:33.33 PS.reconfigure :fast @19:01:33.37 PS.seek 2.5ml @19:01:57.34 PSV.dial PTV @19:01:58.11 delay 5 seconds @19:02:03.13 PSV.dial PSR @19:02:03.87 delay 5 seconds @19:02:08.89 PSV.dial PTV @19:02:09.65 delay 5 seconds @19:02:14.67 PSV.dial :air @19:02:15.44 PS.seek 0ml @19:02:38.98 Gate.power :camera,:ON @19:02:38.99 Mixing DA Substrates @19:02:39.00 PSR.select :air @19:02:39.02 PSV.dial PSR @19:02:40.53 Pulling 0.25ml of PSR.sub1 @19:02:40.56 PSR.select :sub1 @19:02:40.78 PS.reconfigure :default @19:02:40.86 PS.seek 0.25ml @19:02:41.40 Stroke completed -- waiting for pressure to equalize ... @19:02:41.43 SS.reconfigure :exhale @19:02:41.53 SS.hold @19:02:44.24 delay 5 seconds @19:02:49.30 Pulling 0.25ml of PSR.sub2 @19:02:49.31 PSR.select :sub2 @19:02:49.42 PS.seek 0.5ml @19:02:52.99 delay 5 seconds @19:02:58.01 PSR.select :air @19:02:58.11 PS.seek 2ml @19:03:12.29 Intake.select :close @19:03:12.44 SSV.dial :exhaust @19:03:13.21 Exhaust.select :open @19:03:13.32 SS.seek 0ml @19:03:14.27 PTV.dial :puck @19:03:14.29 PSV.dial PTV @19:03:15.04 PBV.dial :waste1 @19:03:15.47 PS.seek 1.5ml @19:03:21.49 delay 3 seconds @19:03:22.15 Exhaust.select :close @19:03:22.26 SSV.dial CBV @19:03:22.29 CBV.dial SSV @19:03:23.06 Allowing 130s for 25.0ml of remaining 125.0ml @19:03:23.07 Intake.select :open @19:03:24.51 PBV.dial between PSV and waste1 @19:03:24.89 PS.seek 0ml @19:03:25.22 Intake @15.7psia, vacuum from 10.0 to 13.0psi @19:03:25.24 SS.reconfigure :inhaling 0.34ml/s @19:03:25.28 SS.seek 25ml @19:03:41.24 delay 10 seconds @19:03:51.27 recoverPSRreagent08 @19:03:51.28 PTV.dial :puck @19:03:51.29 PBV.dial :waste1 @19:03:51.65 delay 30 seconds @19:04:21.67 PBV.dial between PSV and waste1 @19:04:22.06 PSR.select :air @19:04:22.07 PSV.dial PSR @19:04:22.86 PS.reconfigure :fast @19:04:22.90 PS.seek 2.5ml @19:04:34.00 Vacuum too high -- waiting up to 40 seconds @19:04:34.01 SS.reconfigure :exhale @19:04:34.06 SS.hold @19:04:46.99 PSV.dial PTV @19:04:47.76 delay 5 seconds @19:04:48.49 Intake @15.4psia, vacuum from 9.9 to 12.9psi @19:04:48.51 SS.reconfigure :inhaling 0.31ml/s @19:04:48.55 SS.seek 25ml @19:04:52.77 PSV.dial PSR @19:04:53.54 delay 5 seconds @19:04:58.56 PSV.dial PTV @19:04:59.32 delay 5 seconds @19:05:04.34 PSV.dial :air @19:05:05.12 PS.seek 0ml @19:05:10.43 Stroke completed -- waiting for pressure to equalize ... @19:05:10.44 SS.reconfigure :exhale @19:05:10.48 SS.hold @19:05:28.87 Additional air clear @19:05:28.88 PSR.select :air @19:05:28.89 PSV.dial PSR @19:05:30.28 Pulling 2ml of PSR.air @19:05:30.29 PSR.select :air @19:05:30.31 PS.reconfigure :default @19:05:30.35 PS.seek 2ml @19:05:33.08 SS.reconfigure :default @19:05:33.12 SS.hold @19:05:33.23 Intake.select :close @19:05:33.38 ShallowSampler::Clogged in WCR -- Filtering too Slowly after 900.0ml @19:05:33.39 SSV.dial :exhaust @19:05:34.15 Exhaust.select :open @19:05:34.26 SS.reconfigure :exhale @19:05:34.30 SS.seek 0ml @19:05:43.18 Exhaust.select :close @19:05:43.28 Sampled 900.0ml @19:05:43.30 Drying puck before fixing for 20:00 @19:05:43.31 CBV.dial :waste2b @19:05:44.07 delay 1:00 @19:05:51.58 delay 5 seconds @19:05:56.60 PTV.dial :puck @19:05:56.62 PSV.dial PTV @19:05:57.36 PBV.dial :waste1 @19:05:57.81 PS.seek 1.5ml @19:06:03.68 delay 3 seconds @19:06:06.69 PBV.dial between PSV and waste1 @19:06:07.07 delay 1 second @19:06:08.09 PSV.dial :waste1 @19:06:09.16 PS.seek 0ml @19:06:25.41 Processing Puck Heater at 27.9C before imaging @19:06:25.42 PH.coast @19:06:25.54 Imaging da puck in Processing Clamp while storing it to tube 1 @19:06:25.70 SC.reconfigure :tight @19:06:25.75 SC.seek 1 @19:06:29.47 SC.configure :limp @19:06:29.66 Elbow.reconfigure :tight @19:06:29.71 Elbow.seek 1 @19:06:31.54 Forearm.reconfigure :tight @19:06:31.58 Forearm.seek 1 + 450 counts @19:06:32.75 SE.reconfigure :pick @19:06:32.80 SE.seek :beamBreak @19:06:35.58 SE.configure :default @19:06:35.72 Elbow.reconfigure :limp @19:06:35.77 Forearm.reconfigure :loose @19:06:35.81 Forearm.seek 1 @19:06:36.05 Elbow.hold @19:06:36.11 Elbow.reconfigure :tight @19:06:36.20 SE.seek 5.16 Pucks below Top Plate @19:06:36.65 Forearm.force -20 @19:06:36.68 Forearm.seek 1 + 294 counts @19:06:36.98 Forearm.reconfigure :tight @19:06:37.02 Forearm.seek 1 + 450 counts @19:06:37.21 SE.seek 6.16 Pucks below Top Plate @19:06:37.76 Forearm.reconfigure :loose @19:06:38.34 Forearm.seek :retract @19:06:39.27 Elbow.seek PC @19:06:41.83 Elbow.reconfigure :loose @19:06:41.92 Forearm.seek PC @19:06:42.85 Hand.close @19:06:43.48 PC.seek :open @19:06:44.09 CSV.dial :air @19:06:44.56 CS.reconfigure :fast @19:06:44.61 CS.seek 10ml @19:06:48.24 Forearm.seek :retract @19:06:49.21 Elbow.reconfigure :tight @19:06:49.25 Elbow.seek 1 @19:06:51.85 Forearm.reconfigure :tight @19:06:51.89 Forearm.seek 1 + 450 counts @19:06:53.02 Hand.open @19:06:53.66 SE.seek 5.81 Pucks below Top Plate @19:06:54.05 SE.reconfigure :seal @19:06:54.10 SE.seek 4.84 Pucks below Top Plate @19:06:54.58 Lights, camera, ACTION! @19:06:54.62 PuckCamera: snap -bin=2,2 -auto /var/log/esp/da22nov0919h950ml.tif @19:07:09.16 Pumping wcr puck from tube 2 up to 35psi... @19:07:09.17 CBV.dial SSV @19:07:10.48 CBV.dial between CSV and waste2b @19:07:11.46 CSV.dial between air and CTV @19:07:11.80 CS.seek 7.255ml @19:07:14.15 Waiting >20 seconds for da to relinquish FlushPuck @19:07:14.27 SX HX9/16 (USB:02): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 16.174 seconds /var/log/esp/da22nov0919h950ml.tif: TIFF Upload Complete @19:07:14.29 PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da22nov0919h950ml15s.tif @19:07:18.94 CTV.dial CSV,avoiding: :air @19:07:18.97 CSV.dial CTV @19:07:19.80 CS.reconfigure :pressurize @19:07:19.85 CS.seek 0ml @19:07:29.90 SX HX9/16 (USB:02): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15 seconds /var/log/esp/midres/da22nov0919h950ml15s.tif: TIFF Upload Complete @19:07:29.93 PuckCamera: snap -bin=2,2 20.0 /var/log/esp/midres/da22nov0919h950ml20s.tif @19:07:34.86 CBV.dial :waste2b @19:07:35.20 CS.reconfigure :default @19:07:35.25 CS.seek 0ml @19:07:40.03 delay 1:00 @19:07:50.58 SX HX9/16 (USB:02): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 20 seconds /var/log/esp/midres/da22nov0919h950ml20s.tif: TIFF Upload Complete @19:07:50.60 Gate.power :camera,:OFF @19:07:50.62 DAfans.email "16.174 sec auto-exposure",Subject: "da22nov0919h950ml images!" @19:07:50.84 Elbow.seek 1 @19:07:51.05 Forearm.seek 1 + 450 counts @19:07:51.20 SE.reconfigure :gently @19:07:51.24 SE.seek 5.56 Pucks below Top Plate @19:07:51.98 Elbow.reconfigure :limp @19:07:52.02 Forearm.reconfigure :loose @19:07:52.07 Forearm.seek 1 @19:07:52.35 Elbow.hold @19:07:52.41 Elbow.reconfigure :tight @19:07:52.47 SE.reconfigure :default @19:07:52.52 SE.seek 5.74 Pucks below Top Plate @19:07:52.86 Forearm.force -20 @19:07:52.90 Forearm.seek 1 + 289 counts @19:07:53.19 Forearm.reconfigure :tight @19:07:53.24 Forearm.seek 1 + 450 counts @19:07:53.49 SE.seek :down @19:07:54.64 Forearm.reconfigure :loose @19:07:54.68 Forearm.seek :retract @19:07:55.81 SE.stop @19:07:57.04 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @19:07:57.43 Elbow.seek FlushPuck::Garage @19:07:59.48 Elbow.reconfigure :loose @19:07:59.69 Forearm.seek :garage @19:08:00.76 Hand.close @19:08:01.61 Forearm.seek :retract @19:08:02.65 Elbow.reconfigure :tight @19:08:02.74 Elbow.seek PC @19:08:04.09 Elbow.reconfigure :loose @19:08:04.29 Forearm.reconfigure :tight @19:08:04.38 Forearm.seek PC @19:08:06.45 PC.seek :closed @19:08:11.20 Processing Clamp onSpring/offSpring for 16/26 samples, on/off torque 1.56 @19:08:11.35 Hand.open @19:08:12.12 Forearm.reconfigure :loose @19:08:12.21 Forearm.seek :retract @19:08:13.24 PSR.select :air @19:08:13.28 PSV.dial PSR @19:08:13.93 Pulling 0.5ml of PSR.flush @19:08:13.95 PSR.select :flush @19:08:14.14 PS.seek 0.5ml @19:08:20.11 delay 5 seconds @19:08:25.16 PSR.select :air @19:08:25.32 PS.seek 2.5ml @19:08:26.46 upsync @19:08:40.05 CTV.dial :air,avoiding: :intake @19:08:41.40 Fix #1 of 2 (with RNAlater) for 10:00 @19:08:41.42 CSV.dial CSR @19:08:41.91 Pulling 2ml of CSR.RNAlater @19:08:41.92 CSR.select :RNAlater @19:08:42.03 CS.seek 2ml @19:08:46.58 delay 1 second @19:08:47.60 PRV.dial :waste2,avoiding: :ab2 @19:08:47.90 delay 5 seconds @19:08:49.47 PTV.dial :PRVmixing @19:08:50.78 PSV.dial :mixloop @19:08:51.95 PS.seek :empty @19:08:52.91 CSR.select :air @19:08:53.02 CS.reconfigure :fast @19:08:53.07 CS.seek 10ml @19:09:12.62 Pumping wcr puck from tube 2 up to 35psi... @19:09:12.63 CBV.dial SSV @19:09:13.95 CBV.dial between CSV and waste2b @19:09:14.91 CSV.dial between air and CTV @19:09:15.52 CS.seek 7.204ml @19:09:18.24 PSR.select :air @19:09:18.26 PSV.dial PSR @19:09:19.35 Pulling 0.5ml of PSR.flush @19:09:19.36 PSR.select :flush @19:09:19.48 PS.seek 0.5ml @19:09:22.75 CTV.dial CSV,avoiding: :air @19:09:22.77 CSV.dial CTV @19:09:24.15 CS.reconfigure :pressurize @19:09:24.20 CS.seek 0ml @19:09:25.43 delay 5 seconds @19:09:30.44 PSR.select :air @19:09:30.55 PS.seek 2.5ml @19:09:40.42 delay 10:00 @19:09:51.77 delay 1 second @19:09:52.78 PBV.dial :waste1 @19:09:53.14 PTV.dial :mixing @19:09:53.91 PSV.dial :mixloop @19:09:54.97 PS.seek :empty @19:10:21.26 PSV.dial PSR @19:10:22.31 PSR.select :flush @19:10:22.43 PS.seek 1ml @19:10:33.67 PSR.select :air @19:10:33.78 PS.seek 2.5ml @19:10:49.92 PSV.dial PTV @19:10:50.67 PTV.dial :puck @19:10:51.41 PBV.dial :waste1 @19:10:51.90 PS.seek 0ml @19:11:18.23 PSV.dial PSR @19:11:18.98 PSR.select :flush @19:11:19.10 PS.seek 0.5ml @19:11:24.98 PSR.select :air @19:11:25.10 PS.seek 2.5ml @19:11:46.36 PSV.dial PTV @19:11:47.11 PBV.dial PSV @19:11:47.68 PS.seek 0ml @19:12:14.20 PSV.dial PBV @19:12:14.68 PS.seek 2.5ml @19:12:41.07 PSV.dial :waste1 @19:12:41.82 PS.seek 0ml @19:13:08.20 PSV.dial PSR @19:13:08.67 PSR.select :air @19:13:08.69 PS.seek 2.5ml @19:13:35.10 PSV.dial PTV @19:13:35.96 PBV.dial PSV @19:13:35.98 PS.seek 0ml @19:14:02.51 PSV.dial PBV @19:14:02.97 PS.seek 2.5ml @19:14:29.35 PSV.dial :waste1 @19:14:30.10 PS.seek 0ml @19:14:56.63 PSV.dial PSR @19:14:57.10 PSR.select :flush @19:14:57.22 PS.seek 1ml @19:15:08.26 PSR.select :air @19:15:08.37 PS.seek 2.5ml @19:15:24.60 PSV.dial PTV @19:15:25.46 PTV.dial :puck @19:15:25.48 PBV.dial :waste1 @19:15:26.03 PS.seek 0ml @19:15:52.48 PSV.dial PSR @19:15:53.21 PSR.select :flush @19:15:53.33 PS.seek 0.5ml @19:15:59.19 PSR.select :air @19:15:59.31 PS.seek 2.5ml @19:16:20.63 PSV.dial PTV @19:16:21.38 PBV.dial PSV @19:16:21.95 PS.seek 0ml @19:16:48.51 PSV.dial PBV @19:16:48.98 PS.seek 2.5ml @19:17:15.32 PSV.dial :waste1 @19:17:16.06 PS.seek 0ml @19:17:42.44 PSV.dial PSR @19:17:42.91 PSR.select :air @19:17:42.94 PS.seek 2.5ml @19:18:09.32 PSV.dial PTV @19:18:10.05 PBV.dial PSV @19:18:10.07 PS.seek 0ml @19:18:36.44 PSV.dial PBV @19:18:36.92 PS.seek 2.5ml @19:19:03.28 PSV.dial :waste1 @19:19:04.01 PS.seek 0ml @19:19:30.32 Garaging FlushPuck in Processing Clamp @19:19:30.39 Elbow.reconfigure :tight @19:19:30.43 Elbow.seek PC @19:19:30.55 Elbow.reconfigure :loose @19:19:30.64 Forearm.seek PC @19:19:31.59 Hand.close @19:19:32.21 PC.seek :open @19:19:36.92 Forearm.seek :retract @19:19:37.92 Elbow.reconfigure :tight @19:19:37.96 Elbow.seek :garage @19:19:39.25 Elbow.reconfigure :loose @19:19:39.34 Forearm.reconfigure :tight @19:19:39.38 Forearm.seek :garage @19:19:40.38 Hand.open @19:19:40.43 CBV.dial :waste2b @19:19:40.78 CS.reconfigure :default @19:19:40.83 CS.seek 0ml @19:19:41.02 Forearm.reconfigure :loose @19:19:41.07 Forearm.seek :retract @19:19:44.32 delay 1:00 @19:20:44.35 CTV.dial :air,avoiding: :intake @19:20:45.69 Fix #2 of 2 (with RNAlater) for 10:00 @19:20:45.70 CSV.dial CSR @19:20:46.19 Pulling 2ml of CSR.RNAlater @19:20:46.20 CSR.select :RNAlater @19:20:46.31 CS.seek 2ml @19:20:52.65 delay 5 seconds @19:20:57.68 CSR.select :air @19:20:57.78 CS.reconfigure :fast @19:20:57.82 CS.seek 10ml @19:21:17.45 Pumping wcr puck from tube 2 up to 35psi... @19:21:17.46 CBV.dial SSV @19:21:18.76 CBV.dial between CSV and waste2b @19:21:19.72 CSV.dial between air and CTV @19:21:20.32 CS.seek 7.187ml @19:21:27.49 CTV.dial CSV,avoiding: :air @19:21:27.51 CSV.dial CTV @19:21:28.87 CS.reconfigure :pressurize @19:21:28.92 CS.seek 0ml @19:21:46.91 delay 10:00 @19:31:46.93 CBV.dial :waste2b @19:31:47.25 CS.reconfigure :default @19:31:47.30 CS.seek 0ml @19:31:48.91 delay 1:00 @19:32:48.93 CTV.dial :air,avoiding: :intake @19:32:50.32 Storing wcr puck in Collection Clamp to tube 1 @19:32:50.48 SC.reconfigure :tight @19:32:50.53 SC.seek 1 @19:32:50.74 SC.configure :limp @19:32:50.94 Elbow.reconfigure :tight @19:32:50.99 Elbow.seek 1 @19:32:52.83 Forearm.reconfigure :tight @19:32:52.88 Forearm.seek 1 + 450 counts @19:32:53.90 SE.reconfigure :pick @19:32:53.95 SE.seek :beamBreak @19:32:56.73 SE.configure :default @19:32:56.87 Elbow.reconfigure :limp @19:32:56.92 Forearm.reconfigure :loose @19:32:56.97 Forearm.seek 1 @19:32:57.23 Elbow.hold @19:32:57.29 Elbow.reconfigure :tight @19:32:57.38 SE.seek 6.14 Pucks below Top Plate @19:32:57.84 Forearm.force -20 @19:32:57.87 Forearm.seek 1 + 288 counts @19:32:58.16 Forearm.reconfigure :tight @19:32:58.21 Forearm.seek 1 + 450 counts @19:32:58.50 SE.seek 7.14 Pucks below Top Plate @19:32:59.03 Forearm.reconfigure :loose @19:32:59.07 Forearm.seek :retract @19:33:00.05 Elbow.seek CC @19:33:01.63 Elbow.reconfigure :loose @19:33:01.72 Forearm.seek CC @19:33:02.67 Hand.close @19:33:03.33 CC.seek :open @19:33:08.05 Forearm.seek :retract @19:33:09.00 Elbow.reconfigure :tight @19:33:09.05 Elbow.seek 1 @19:33:10.84 Elbow.reconfigure :limp @19:33:10.89 Forearm.seek 1 @19:33:11.84 Elbow.hold @19:33:11.91 Elbow.reconfigure :tight @19:33:11.95 Hand.open @19:33:12.69 Forearm.force -20 @19:33:12.72 Forearm.seek 1 + 318 counts @19:33:13.46 Forearm.reconfigure :tight @19:33:13.52 Forearm.seek 1 + 450 counts @19:33:13.77 SE.seek :down @19:33:14.90 Forearm.reconfigure :loose @19:33:14.94 Forearm.seek :retract @19:33:15.98 SE.stop @19:33:16.02
Starting finalPurge @19:33:16.03 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @19:33:16.21 Elbow.seek FlushPuck::Garage @19:33:18.03 Elbow.reconfigure :loose @19:33:18.11 Forearm.seek :garage @19:33:19.06 Hand.close @19:33:19.81 Forearm.seek :retract @19:33:20.81 Elbow.reconfigure :tight @19:33:20.85 Elbow.seek CC @19:33:23.75 Elbow.reconfigure :loose @19:33:23.84 Forearm.reconfigure :tight @19:33:23.88 Forearm.seek CC @19:33:25.06 CC.seek :closed @19:33:29.59 Collection Clamp onSpring/offSpring for 13/27 samples, on/off torque 2.19 @19:33:29.66 Hand.open @19:33:30.38 Forearm.reconfigure :loose @19:33:30.42 Forearm.seek :retract @19:33:31.42 Flushing the Collection Valve::Manifold @19:33:31.54 CSV.dial CSR @19:33:32.13 Pulling 5ml of CSR.flush @19:33:32.14 CSR.select :flush @19:33:32.25 CS.seek 5ml @19:33:46.00 delay 5 seconds @19:33:51.02 CSR.select :air @19:33:51.16 CS.reconfigure :fast @19:33:51.21 CS.seek 7.5ml @19:33:57.90 CSV.dial :waste @19:33:59.16 CS.reconfigure :default @19:33:59.20 CS.seek 0ml @19:34:19.18 Flushing lower manifold's waste1 port @19:34:19.30 CSV.dial :flush @19:34:19.75 CS.reconfigure :fast @19:34:19.79 CS.seek 5ml @19:34:32.56 delay 5 seconds @19:34:37.58 CSV.dial :air @19:34:38.07 CS.seek 7ml @19:34:43.65 CSV.dial CTV @19:34:43.68
CTV.dial CSV @19:34:45.06 CBV.dial :waste1 @19:34:45.81 CS.seek 0ml @19:35:02.40 Flushing lower manifold's waste2 port @19:35:02.64 CSV.dial :flush @19:35:03.52 CS.seek 5ml @19:35:16.31 delay 5 seconds @19:35:21.33 CSV.dial :air @19:35:21.82 CS.seek 7ml @19:35:27.31 CSV.dial CTV @19:35:27.33
CTV.dial CSV @19:35:27.78 CBV.dial :waste2 @19:35:28.83 CS.seek 0ml @19:35:45.56 Flushing lower manifold's waste2b port @19:35:45.67 CSV.dial :flush @19:35:46.41 CS.seek 5ml @19:35:59.09 delay 5 seconds @19:36:04.11 CSV.dial :air @19:36:04.62 CS.seek 7.001ml @19:36:10.09 CSV.dial CTV @19:36:10.11
CTV.dial CSV @19:36:10.56 CBV.dial :waste2b @19:36:11.59 CS.seek 0ml @19:36:28.28 Disinfecting external Intake with kill @19:36:28.39 CSV.dial CSR @19:36:28.88 Pulling 8ml of CSR.kill CSR.select :kill @19:36:28.99 CS.seek 8ml @19:36:48.68 delay 5 seconds @19:36:53.70 CSR.select :air @19:36:53.81 CS.seek 10ml @19:36:59.28 CSV.dial CBV @19:36:59.30
CBV.dial CSV @19:37:00.95 CTV.dial :intake @19:37:01.73 Intake.select :open @19:37:01.84 CS.seek 2ml @19:37:20.71 Intake.select :close @19:37:20.81 CTV.dial :air @19:37:21.56 CS.reconfigure :default @19:37:21.60 CS.seek 0ml @19:37:27.74 CSV.dial CSR @19:37:28.80 Pulling 5ml of CSR.flush @19:37:28.81 CSR.select :flush @19:37:28.92 CS.seek 5ml @19:37:42.72 delay 5 seconds @19:37:47.73 CSR.select :air @19:37:47.85 CS.seek 10ml @19:38:01.40 CSV.dial CBV @19:38:02.41 CS.seek 0ml @19:38:28.78 Garaging FlushPuck in Collection Clamp @19:38:28.84 Elbow.reconfigure :tight @19:38:28.89 Elbow.seek CC @19:38:29.23 Elbow.reconfigure :loose @19:38:29.32 Forearm.seek CC @19:38:30.35 Hand.close @19:38:30.95 CC.seek :open @19:38:35.57 Forearm.seek :retract @19:38:36.60 Elbow.reconfigure :tight @19:38:36.65 Elbow.seek :garage @19:38:39.56 Elbow.reconfigure :loose @19:38:39.65 Forearm.reconfigure :tight @19:38:39.70 Forearm.seek :garage @19:38:40.72 Hand.open @19:38:41.38 Forearm.reconfigure :loose @19:38:41.42 Forearm.seek :retract @19:38:42.39 Completed [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] begun 16:50:32 @19:38:42.43 Asleep after completing DA_WCR_110822 assays @19:38:42.45 GoodNews.email "{1 => 8,\n 2 => 25,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@19:29:43, 18% flow, 16.3C, 23% humidity, 14.6psia, 13.759V, 0.460A, 0.447A avg, 6.32W\nSampled 900.0ml",Subject: "Asleep after completing DA_WCR_110822 assays" @19:38:47.85 upsync @18:31:09.24UTC16-Nov-22
ESP2 v3.41 I2C Gateway v3.20 for core bus @18:31:11.80 Configuration for short puck jake 1/11/21 by brent@mbari.org /home/esp/esp2/type/shallow/jake/configure.rb @18:31:15.62 Polling Can every 10:00 @18:31:15.63 Cleanly exited at 18:28:47UTC16-Nov-22 @18:31:15.67 Loading /home/esp/esp2/mission/DA_WCR_111622.rb mission @18:31:15.72 GoodNews.email "Starting DA_WCR_111622 w/pucks in tube 2\n{}\nCTD data unavailable\nCan@18:31:15, 17% flow, 16.0C, 20% humidity, 14.6psia, 13.764V, 0.363A, 0.345A avg, 4.99W",Subject: "DA_WCR_111622 begun" @18:31:15.75
Preparing to sleep until + 00:00:05 @18:31:15.79 Asleep til + 00:00:05 @18:31:15.81 Continuing at 18:31 @18:31:16.10 Gate.power :core,:OFF @18:31:16.11 Gate.power :raw,:OFF @18:31:16.12 Shutdown @18:31:19.89 GoodNews.email "Then, in tube 2: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2/mission/DA_WCR_111622.rb:14\n{1 => 8,\n 2 => 25,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@18:31:15, 17% flow, 16.0C, 20% humidity, 14.6psia, 13.764V, 0.363A, 0.345A avg, 4.99W",Subject: "Asleep until 18:31" @18:31:20.69
Powering core bus @18:31:22.07 upsync @18:31:25.30 upsync @18:31:26.24
Gate.power :raw,:ON @18:31:27.25 CS.configure :default @18:31:27.35 PS.configure :default @18:31:27.44 SS.configure :default @18:31:27.53 CC.configure :default @18:31:27.61 PC.configure :default @18:31:27.72 Forearm.configure :loose @18:31:27.80 Elbow.configure :loose @18:31:27.91 SC.configure :loose @18:31:28.00 SE.configure :default @18:31:28.04 CH.coast @18:31:28.10 CH.configure :default @18:31:28.13 PH.coast @18:31:28.18 PH.configure :default @18:31:28.21 All dwarf controllers running firmware version 3.85 @18:31:28.31 Valve.reset @18:31:28.52 Intake.select :close @18:31:28.59 Exhaust.select :close @18:31:28.61 CTV.dial :air @18:31:28.69 PSV.dial :air @18:31:28.76 CSV.dial :waste1 @18:31:28.84
Forearm.reconfigure :loose @18:31:28.89 Forearm.home.seek :retract @18:31:29.21 SSV.dial :spit,avoiding: :exhaust @18:31:29.62 Hand.close @18:31:29.66
Elbow.jog 1500 @18:31:29.79 PS.reconfigure :default @18:31:29.84 PS.home.seek 0ml @18:31:30.02 CS.reconfigure :fast @18:31:30.06 SS.reconfigure :default @18:31:30.14 CS.home.seek 0ml @18:31:30.18 SS.home.seek 0ml @18:31:30.29
Elbow.reconfigure :loose @18:31:30.34 Elbow.home.seek :clear @18:31:33.60 SE.reconfigure :default @18:31:33.64 SE.home.seek :down @18:31:34.24 SC.reconfigure :tight @18:31:34.28 SC.home.seek :initial @18:31:38.19 PS.stop @18:32:02.65
SC.configure :limp @18:32:02.70 Forearm.seek :retract @18:32:02.76 Elbow.seek :hammer @18:32:03.77 Forearm.reconfigure :tight @18:32:03.81 Forearm.seek retracted @18:32:04.06 PC.reconfigure :default @18:32:04.08
CC.reconfigure :default @18:32:04.17 CC.home.seek :open @18:32:04.19 PC.home.seek :open @18:32:07.71
Phase [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] @18:32:07.72 Notice.email "At 18:32:07 began in tube 2: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2/mission/DA_WCR_111622.rb:14\n{1 => 8,\n 2 => 25,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@18:32:07, 18% flow, 16.0C, 19% humidity, 14.6psia, 13.759V, 0.321A, 0.302A avg, 4.41W",Subject: "Started DA_WCR_111622 phase initialPurge; da [1000, 1000] #da and wsr are run simultanelously" @18:32:07.74
Starting initialPurge @18:32:07.76 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @18:32:07.83 Hand.open @18:32:08.50 Elbow.reconfigure :tight @18:32:08.55 Elbow.seek FlushPuck::Garage @18:32:10.40 Elbow.reconfigure :loose @18:32:10.52 Forearm.reconfigure :loose @18:32:10.61 Forearm.seek :garage @18:32:11.60 Hand.close @18:32:12.39 Forearm.seek :retract @18:32:13.39 Elbow.reconfigure :tight @18:32:13.41 upsync @18:32:13.46
Elbow.seek CC @18:32:16.39 Elbow.reconfigure :loose @18:32:16.48 Forearm.reconfigure :tight @18:32:16.52 Forearm.seek CC @18:32:17.78 CC.seek :closed @18:32:22.39 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.24 @18:32:22.46 Hand.open @18:32:23.20 Forearm.reconfigure :loose @18:32:23.24 Forearm.seek :retract @18:32:24.19 Flushing the Collection Valve::Manifold @18:32:24.31 CSV.dial CSR @18:32:25.63 Pulling 5ml of CSR.flush @18:32:25.64 CSR.select :flush @18:32:25.75 CS.reconfigure :default @18:32:25.80 CS.seek 5ml @18:32:39.39 delay 5 seconds @18:32:44.42 CSR.select :air @18:32:44.56 CS.reconfigure :fast @18:32:44.61 CS.seek 7.5ml @18:32:51.39 CSV.dial :waste @18:32:52.66 CS.reconfigure :default @18:32:52.70 CS.seek 0ml @18:33:12.67 Purging external Intake @18:33:12.79 CSV.dial :flush @18:33:13.35 CS.reconfigure :fast @18:33:13.39 CS.seek 10ml @18:33:37.86 delay 5 seconds @18:33:42.88 CSV.dial CBV @18:33:42.91
CBV.dial CSV @18:33:43.64 CTV.dial :intake @18:33:44.44 Intake.select :open @18:33:44.54 CS.seek 0ml @18:34:08.17 Intake.select :close @18:34:08.27 CTV.dial :air @18:34:09.05 Flushing upper manifold @18:34:09.16 CSV.dial :flush @18:34:09.90 CS.seek 5ml @18:34:22.67 delay 5 seconds @18:34:27.69 CSV.dial :air @18:34:28.18 CS.seek 7.001ml @18:34:33.80 CSV.dial CBV @18:34:33.84
CBV.dial CSV @18:34:34.83 CTV.dial :air @18:34:34.85 CS.seek 0ml @18:34:51.61 delay 7 seconds @18:34:58.63 CS.seek 3ml @18:35:06.61 CSV.dial :waste @18:35:07.05 CS.seek 0ml @18:35:14.87 CSV.dial :flush @18:35:15.30 CS.seek 3ml @18:35:23.26 delay 5 seconds @18:35:28.28 CSV.dial :air @18:35:28.78 CS.seek 5ml @18:35:34.45 CSV.dial :air @18:35:34.47 CS.seek 0ml @18:35:46.79 delay 7 seconds @18:35:53.82 CS.seek 3ml @18:36:01.70 CSV.dial :waste @18:36:02.43 CS.seek 0ml @18:36:10.17 Garaging FlushPuck in Collection Clamp @18:36:10.24 Elbow.reconfigure :tight @18:36:10.29 Elbow.seek CC @18:36:10.40 Elbow.reconfigure :loose @18:36:10.49 Forearm.seek CC @18:36:11.47 Hand.close @18:36:12.13 CC.seek :open @18:36:16.78 Forearm.seek :retract @18:36:17.79 Elbow.reconfigure :tight @18:36:17.83 Elbow.seek :garage @18:36:20.85 Elbow.reconfigure :loose @18:36:20.94 Forearm.reconfigure :tight @18:36:20.99 Forearm.seek :garage @18:36:21.88 Hand.open @18:36:22.93 Forearm.reconfigure :loose @18:36:22.98 Forearm.seek :retract @18:36:23.94 da sampling at most 1000ml, wcr at most 1000ml @18:36:23.98 Loading sh1 puck from tube 2 into Collection Clamp @18:36:23.99 DAfans.email "Started da in tube 2 at 18:36:23UTC16-Nov-22\n{1 => 8,\n 2 => 25,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@18:32:07, 18% flow, 16.0C, 19% humidity, 14.6psia, 13.759V, 0.321A, 0.302A avg, 4.41W",Subject: "da sampling at most 1000ml, wcr at most 1000ml" @18:36:24.24
SC.reconfigure :tight @18:36:24.28 SC.seek 2 @18:36:29.95 upsync @18:36:30.97
SC.configure :limp @18:36:31.17 Elbow.reconfigure :tight @18:36:31.22 Elbow.seek 2 @18:36:33.13 Forearm.reconfigure :tight @18:36:33.18 Forearm.seek 2 + 450 counts @18:36:34.19 SE.reconfigure :pick @18:36:34.24 SE.seek :beamBreak @18:36:35.75 SE.configure :default @18:36:35.90 Elbow.seek 2 @18:36:36.08 Forearm.seek 2 + 300 counts @18:36:36.39 Forearm.reconfigure :loose @18:36:36.43 Hand.close @18:36:37.02 SE.seek :down @18:36:37.20
Forearm.seek :retract @18:36:38.13 Elbow.seek CC @18:36:38.19 SE.stop @18:36:39.75
Elbow.reconfigure :loose @18:36:39.84 Forearm.reconfigure :tight @18:36:39.88 Forearm.seek CC @18:36:41.17 CC.seek :closed @18:36:45.68 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.25 @18:36:45.69 Hand.open @18:36:46.32 Forearm.reconfigure :loose @18:36:46.36 Forearm.seek :retract @18:36:47.33 collectSample 1000ml w/bubble point of 24psi @18:36:47.35 Priming 7ml Intake.select :close @18:36:47.36 Exhaust.select :close @18:36:47.37 SS.hold @18:36:47.56 Calibrating pressure and temperature sensors @18:36:47.59 CTV.dial :air @18:36:47.61 SSV.dial :spit,avoiding: :exhaust @18:36:47.62 SS.reconfigure :fast @18:36:47.67 SS.seek 5ml @18:36:50.51 SSV.dial CBV @18:36:50.53
CBV.dial SSV @18:36:51.85 delay 3.8 seconds @18:36:55.77 SSV.dial :spit @18:36:57.10 SS.seek 0ml @18:37:00.09 SSV.dial CBV @18:37:01.38 CTV.dial :intake @18:37:02.16 SSV.dial CBV @18:37:02.18
CBV.dial SSV @18:37:02.20 Allowing 40s for 7.0ml of remaining 7.0ml @18:37:02.21 Intake.select :open @18:37:04.36 Intake @14.5psia, vacuum from 9.0 to 12.0psi @18:37:04.38 SS.reconfigure :inhaling 2.20ml/s @18:37:04.42 SS.seek 7ml @18:37:10.68 Stroke completed -- waiting for pressure to equalize ... @18:37:10.69 SS.reconfigure :exhale @18:37:10.73 SS.hold @18:37:13.13 Intake.select :close @18:37:13.27 SSV.dial :exhaust @18:37:14.02 Exhaust.select :open @18:37:14.12 SS.seek 0ml @18:37:17.96 Exhaust.select :close @18:37:18.06 Sampling 1000.0ml @18:37:18.07 Intake.select :close @18:37:18.08 Exhaust.select :close @18:37:18.09 SS.reconfigure :default @18:37:18.13 SS.hold @18:37:18.31 CTV.dial :intake @18:37:18.33 SSV.dial CBV @18:37:18.35
CBV.dial SSV @18:37:19.11 Allowing 130s for 25.0ml of remaining 1000.0ml @18:37:19.12 Intake.select :open @18:37:21.27 Intake @15.3psia, vacuum from 9.8 to 12.8psi @18:37:21.29 SS.reconfigure :inhaling 2.20ml/s @18:37:21.33 SS.seek 25ml @18:37:32.14 Vacuum too high -- waiting up to 40 seconds @18:37:32.15 SS.reconfigure :exhale @18:37:32.19 SS.hold @18:37:36.88 Intake @14.0psia, vacuum from 8.5 to 11.5psi @18:37:36.90 SS.reconfigure :inhaling 1.98ml/s @18:37:36.94 SS.seek 25ml @18:37:42.49 Stroke completed -- waiting for pressure to equalize ... @18:37:42.50 SS.reconfigure :exhale @18:37:42.54 SS.hold @18:37:49.05 Intake.select :close @18:37:49.18 SSV.dial :exhaust @18:37:49.93 Exhaust.select :open @18:37:50.04 SS.seek 0ml @18:37:59.01 Exhaust.select :close @18:37:59.11 SSV.dial CBV @18:37:59.13
CBV.dial SSV @18:37:59.88 Allowing 130s for 25.0ml of remaining 975.0ml @18:37:59.89 Intake.select :open @18:38:02.05 Intake @15.9psia, vacuum from 10.0 to 13.0psi @18:38:02.06 SS.reconfigure :inhaling 1.98ml/s @18:38:02.10 SS.seek 25ml @18:38:10.14 Vacuum too high -- waiting up to 40 seconds @18:38:10.15 SS.reconfigure :exhale @18:38:10.19 SS.hold @18:38:12.93 Intake @14.2psia, vacuum from 8.7 to 11.7psi @18:38:12.95 SS.reconfigure :inhaling 1.79ml/s @18:38:12.99 SS.seek 25ml @18:38:22.44 Stroke completed -- waiting for pressure to equalize ... @18:38:22.45 SS.reconfigure :exhale @18:38:22.49 SS.hold @18:38:32.15 Intake.select :close @18:38:32.29 SSV.dial :exhaust @18:38:33.02 Exhaust.select :open @18:38:33.13 SS.seek 0ml @18:38:41.91 Exhaust.select :close @18:38:42.02 SSV.dial CBV @18:38:42.03
CBV.dial SSV @18:38:42.79 Allowing 130s for 25.0ml of remaining 950.0ml @18:38:42.80 Intake.select :open @18:38:44.95 Intake @16.0psia, vacuum from 10.0 to 13.0psi @18:38:44.96 SS.reconfigure :inhaling 1.79ml/s @18:38:45.01 SS.seek 25ml @18:38:53.95 Vacuum too high -- waiting up to 40 seconds @18:38:53.96 SS.reconfigure :exhale @18:38:54.00 SS.hold @18:38:56.66 Intake @14.3psia, vacuum from 8.8 to 11.8psi @18:38:56.68 SS.reconfigure :inhaling 1.61ml/s @18:38:56.72 SS.seek 25ml @18:39:06.69 Stroke completed -- waiting for pressure to equalize ... @18:39:06.70 SS.reconfigure :exhale @18:39:06.74 SS.hold @18:39:16.41 Intake.select :close @18:39:16.55 SSV.dial :exhaust @18:39:17.29 Exhaust.select :open @18:39:17.39 SS.seek 0ml @18:39:26.21 Exhaust.select :close @18:39:26.31 SSV.dial CBV @18:39:26.33
CBV.dial SSV @18:39:27.08 Allowing 130s for 25.0ml of remaining 925.0ml @18:39:27.09 Intake.select :open @18:39:29.24 Intake @16.0psia, vacuum from 10.0 to 13.0psi @18:39:29.26 SS.reconfigure :inhaling 1.61ml/s @18:39:29.30 SS.seek 25ml @18:39:39.54 Vacuum too high -- waiting up to 40 seconds @18:39:39.55 SS.reconfigure :exhale @18:39:39.60 SS.hold @18:39:43.18 Intake @14.4psia, vacuum from 8.9 to 11.9psi @18:39:43.21 SS.reconfigure :inhaling 1.45ml/s @18:39:43.25 SS.seek 25ml @18:39:52.42 Stroke completed -- waiting for pressure to equalize ... @18:39:52.43 SS.reconfigure :exhale @18:39:52.48 SS.hold @18:40:00.15 Intake.select :close @18:40:00.28 SSV.dial :exhaust @18:40:01.02 Exhaust.select :open @18:40:01.13 SS.seek 0ml @18:40:09.88 Exhaust.select :close @18:40:09.98 SSV.dial CBV @18:40:10.00
CBV.dial SSV @18:40:10.76 Allowing 130s for 25.0ml of remaining 900.0ml Intake.select :open @18:40:12.91 Intake @16.0psia, vacuum from 10.0 to 13.0psi @18:40:12.93 SS.reconfigure :inhaling 1.45ml/s @18:40:12.97 SS.seek 25ml @18:40:24.67 Vacuum too high -- waiting up to 40 seconds SS.reconfigure :exhale @18:40:24.71 SS.hold @18:40:27.82 Intake @14.3psia, vacuum from 8.8 to 11.8psi @18:40:27.83 SS.reconfigure :inhaling 1.30ml/s @18:40:27.87 SS.seek 25ml @18:40:38.05 Stroke completed -- waiting for pressure to equalize ... @18:40:38.06 SS.reconfigure :exhale @18:40:38.10 SS.hold @18:40:45.46 Intake.select :close @18:40:45.59 SSV.dial :exhaust @18:40:46.33 Exhaust.select :open @18:40:46.44 SS.seek 0ml @18:40:55.45 Exhaust.select :close @18:40:55.56 SSV.dial CBV @18:40:55.58
CBV.dial SSV @18:40:56.33 Allowing 130s for 25.0ml of remaining 875.0ml @18:40:56.34 Intake.select :open @18:40:58.50 Intake @16.0psia, vacuum from 10.0 to 13.0psi @18:40:58.51 SS.reconfigure :inhaling 1.30ml/s @18:40:58.56 SS.seek 25ml @18:41:13.10 Vacuum too high -- waiting up to 40 seconds @18:41:13.11 SS.reconfigure :exhale @18:41:13.16 SS.hold @18:41:16.73 Intake @14.6psia, vacuum from 9.1 to 12.1psi @18:41:16.74 SS.reconfigure :inhaling 1.17ml/s @18:41:16.79 SS.seek 25ml @18:41:25.14 Stroke completed -- waiting for pressure to equalize ... @18:41:25.15 SS.reconfigure :exhale @18:41:25.19 SS.hold @18:41:31.60 Intake.select :close @18:41:31.73 SSV.dial :exhaust @18:41:32.47 Exhaust.select :open @18:41:32.58 SS.seek 0ml @18:41:41.23 Exhaust.select :close @18:41:41.34 SSV.dial CBV @18:41:41.38
CBV.dial SSV @18:41:42.10 Allowing 130s for 25.0ml of remaining 850.0ml @18:41:42.11 Intake.select :open @18:41:44.26 Intake @16.0psia, vacuum from 10.0 to 13.0psi @18:41:44.27 SS.reconfigure :inhaling 1.17ml/s @18:41:44.32 SS.seek 25ml @18:42:03.48 Vacuum too high -- waiting up to 40 seconds @18:42:03.49 SS.reconfigure :exhale @18:42:03.53 SS.hold @18:42:07.08 Intake @14.6psia, vacuum from 9.1 to 12.1psi @18:42:07.10 SS.reconfigure :inhaling 1.06ml/s @18:42:07.14 SS.seek 25ml @18:42:13.41 Stroke completed -- waiting for pressure to equalize ... @18:42:13.42 SS.reconfigure :exhale @18:42:13.46 SS.hold @18:42:19.19 Intake.select :close @18:42:19.32 SSV.dial :exhaust @18:42:20.07 Exhaust.select :open @18:42:20.17 SS.seek 0ml @18:42:28.94 Exhaust.select :close @18:42:29.04 SSV.dial CBV @18:42:29.06
CBV.dial SSV @18:42:29.82 Allowing 130s for 25.0ml of remaining 825.0ml Intake.select :open @18:42:31.97 Intake @15.9psia, vacuum from 10.0 to 13.0psi @18:42:31.99 SS.reconfigure :inhaling 1.06ml/s @18:42:32.03 SS.seek 25ml @18:42:58.48 Stroke completed -- waiting for pressure to equalize ... @18:42:58.49 SS.reconfigure :exhale @18:42:58.54 SS.hold @18:43:07.07 Intake.select :close @18:43:07.21 SSV.dial :exhaust @18:43:07.95 Exhaust.select :open @18:43:08.06 SS.seek 0ml @18:43:16.69 Exhaust.select :close @18:43:16.79 SSV.dial CBV @18:43:16.81
CBV.dial SSV @18:43:17.56 Allowing 130s for 25.0ml of remaining 800.0ml @18:43:17.57 Intake.select :open @18:43:19.73 Intake @16.0psia, vacuum from 10.0 to 13.0psi @18:43:19.75 SS.reconfigure :inhaling 1.06ml/s @18:43:19.79 SS.seek 25ml @18:43:44.29 Vacuum too high -- waiting up to 40 seconds @18:43:44.30 SS.reconfigure :exhale @18:43:44.34 SS.hold @18:43:48.50 Intake @14.9psia, vacuum from 9.4 to 12.4psi @18:43:48.51 SS.reconfigure :inhaling 0.95ml/s @18:43:48.56 SS.seek 25ml @18:43:51.29 Stroke completed -- waiting for pressure to equalize ... @18:43:51.30 SS.reconfigure :exhale @18:43:51.35 SS.hold @18:43:56.11 Intake.select :close @18:43:56.25 SSV.dial :exhaust @18:43:57.00 Exhaust.select :open @18:43:57.11 SS.seek 0ml @18:44:05.81 Exhaust.select :close @18:44:05.91 SSV.dial CBV @18:44:05.93
CBV.dial SSV @18:44:06.69 Allowing 130s for 25.0ml of remaining 775.0ml @18:44:06.70 Intake.select :open @18:44:08.85 Intake @15.8psia, vacuum from 10.0 to 13.0psi @18:44:08.87 SS.reconfigure :inhaling 0.95ml/s @18:44:08.91 SS.seek 25ml @18:44:37.81 Stroke completed -- waiting for pressure to equalize ... @18:44:37.82 SS.reconfigure :exhale @18:44:37.86 SS.hold @18:44:46.41 Intake.select :close @18:44:46.54 SSV.dial :exhaust @18:44:47.28 Exhaust.select :open @18:44:47.39 SS.seek 0ml @18:44:56.43 Exhaust.select :close @18:44:56.54 SSV.dial CBV @18:44:56.56
CBV.dial SSV @18:44:57.31 Allowing 130s for 25.0ml of remaining 750.0ml @18:44:57.32 Intake.select :open @18:44:59.47 Intake @16.0psia, vacuum from 10.0 to 13.0psi @18:44:59.49 SS.reconfigure :inhaling 0.95ml/s @18:44:59.53 SS.seek 25ml @18:45:27.64 Stroke completed -- waiting for pressure to equalize ... @18:45:27.65 SS.reconfigure :exhale @18:45:27.69 SS.hold @18:45:36.71 Intake.select :close @18:45:36.85 SSV.dial :exhaust @18:45:37.59 Exhaust.select :open @18:45:37.70 SS.seek 0ml @18:45:46.50 Exhaust.select :close @18:45:46.60 SSV.dial CBV @18:45:46.62
CBV.dial SSV @18:45:47.38 Allowing 130s for 25.0ml of remaining 725.0ml Intake.select :open @18:45:49.53 Intake @15.8psia, vacuum from 10.0 to 13.0psi @18:45:49.55 SS.reconfigure :inhaling 0.95ml/s @18:45:49.59 SS.seek 25ml @18:46:18.78 Stroke completed -- waiting for pressure to equalize ... @18:46:18.79 SS.reconfigure :exhale @18:46:18.83 SS.hold @18:46:28.61 Intake.select :close @18:46:28.75 SSV.dial :exhaust @18:46:29.48 Exhaust.select :open @18:46:29.59 SS.seek 0ml @18:46:38.29 Exhaust.select :close @18:46:38.39 SSV.dial CBV @18:46:38.41
CBV.dial SSV @18:46:39.17 Allowing 130s for 25.0ml of remaining 700.0ml @18:46:39.18 Intake.select :open @18:46:41.33 Intake @15.7psia, vacuum from 10.0 to 13.0psi @18:46:41.35 SS.reconfigure :inhaling 0.95ml/s @18:46:41.39 SS.seek 25ml @18:47:11.03 Stroke completed -- waiting for pressure to equalize ... @18:47:11.04 SS.reconfigure :exhale @18:47:11.08 SS.hold @18:47:20.81 Intake.select :close @18:47:20.95 SSV.dial :exhaust @18:47:21.70 Exhaust.select :open @18:47:21.81 SS.seek 0ml @18:47:30.62 Exhaust.select :close @18:47:30.72 SSV.dial CBV @18:47:30.74
CBV.dial SSV @18:47:31.50 Allowing 130s for 25.0ml of remaining 675.0ml @18:47:31.51 Intake.select :open @18:47:33.66 Intake @16.0psia, vacuum from 10.0 to 13.0psi @18:47:33.67 SS.reconfigure :inhaling 0.95ml/s @18:47:33.71 SS.seek 25ml @18:47:56.32 Vacuum too high -- waiting up to 40 seconds @18:47:56.33 SS.reconfigure :exhale @18:47:56.37 SS.hold @18:48:01.16 Intake @15.1psia, vacuum from 9.6 to 12.6psi @18:48:01.18 SS.reconfigure :inhaling 0.87ml/s @18:48:01.22 SS.seek 25ml @18:48:08.62 Stroke completed -- waiting for pressure to equalize ... @18:48:08.63 SS.reconfigure :exhale @18:48:08.67 SS.hold @18:48:15.06 Intake.select :close @18:48:15.20 SSV.dial :exhaust @18:48:15.93 Exhaust.select :open @18:48:16.04 SS.seek 0ml @18:48:25.06 Exhaust.select :close @18:48:25.16 SSV.dial CBV @18:48:25.18
CBV.dial SSV @18:48:25.93 Allowing 130s for 25.0ml of remaining 650.0ml @18:48:25.94 Intake.select :open @18:48:28.10 Intake @15.9psia, vacuum from 10.0 to 13.0psi @18:48:28.11 SS.reconfigure :inhaling 0.87ml/s @18:48:28.16 SS.seek 25ml @18:48:59.52 Stroke completed -- waiting for pressure to equalize ... @18:48:59.53 SS.reconfigure :exhale @18:48:59.57 SS.hold @18:49:09.27 Intake.select :close @18:49:09.79 SSV.dial :exhaust @18:49:10.63 Exhaust.select :open @18:49:10.74 SS.seek 0ml @18:49:19.84 Exhaust.select :close @18:49:19.94 SSV.dial CBV @18:49:19.96
CBV.dial SSV @18:49:20.71 Allowing 130s for 25.0ml of remaining 625.0ml @18:49:20.72 Intake.select :open @18:49:22.88 Intake @15.8psia, vacuum from 10.0 to 13.0psi @18:49:22.89 SS.reconfigure :inhaling 0.87ml/s @18:49:22.94 SS.seek 25ml @18:49:54.16 Stroke completed -- waiting for pressure to equalize ... @18:49:54.17 SS.reconfigure :exhale @18:49:54.21 SS.hold @18:50:05.32 Intake.select :close @18:50:05.46 SSV.dial :exhaust @18:50:06.20 Exhaust.select :open @18:50:06.30 SS.seek 0ml @18:50:15.13 Exhaust.select :close @18:50:15.23 SSV.dial CBV @18:50:15.26
CBV.dial SSV @18:50:16.01 Allowing 130s for 25.0ml of remaining 600.0ml @18:50:16.02 Intake.select :open @18:50:18.17 Intake @15.8psia, vacuum from 10.0 to 13.0psi @18:50:18.19 SS.reconfigure :inhaling 0.87ml/s @18:50:18.23 SS.seek 25ml @18:50:49.99 Stroke completed -- waiting for pressure to equalize ... @18:50:50.00 SS.reconfigure :exhale @18:50:50.04 SS.hold @18:51:01.15 Intake.select :close @18:51:01.28 SSV.dial :exhaust @18:51:02.02 Exhaust.select :open @18:51:02.13 SS.seek 0ml @18:51:10.93 Exhaust.select :close @18:51:11.03 SSV.dial CBV @18:51:11.05
CBV.dial SSV @18:51:11.80 Allowing 130s for 25.0ml of remaining 575.0ml @18:51:11.81 Intake.select :open @18:51:13.97 Intake @15.8psia, vacuum from 10.0 to 13.0psi @18:51:13.99 SS.reconfigure :inhaling 0.87ml/s @18:51:14.03 SS.seek 25ml @18:51:42.09 Vacuum too high -- waiting up to 40 seconds @18:51:42.10 SS.reconfigure :exhale @18:51:42.14 SS.hold @18:51:48.67 Intake @15.4psia, vacuum from 9.9 to 12.9psi @18:51:48.69 SS.reconfigure :inhaling 0.78ml/s @18:51:48.73 SS.seek 25ml @18:51:52.92 Stroke completed -- waiting for pressure to equalize ... @18:51:52.93 SS.reconfigure :exhale @18:51:52.97 SS.hold @18:51:59.47 Intake.select :close @18:51:59.62 SSV.dial :exhaust @18:52:00.36 Exhaust.select :open @18:52:00.47 SS.seek 0ml @18:52:09.47 Exhaust.select :close @18:52:09.58 SSV.dial CBV @18:52:09.59
CBV.dial SSV @18:52:10.35 Allowing 130s for 25.0ml of remaining 550.0ml @18:52:10.36 Intake.select :open @18:52:12.51 Intake @15.7psia, vacuum from 10.0 to 13.0psi @18:52:12.53 SS.reconfigure :inhaling 0.78ml/s @18:52:13.03 SS.seek 25ml @18:52:47.55 Stroke completed -- waiting for pressure to equalize ... @18:52:47.56 SS.reconfigure :exhale @18:52:47.60 SS.hold @18:53:00.26 Intake.select :close @18:53:00.39 SSV.dial :exhaust @18:53:01.13 Exhaust.select :open @18:53:01.24 SS.seek 0ml @18:53:10.28 Exhaust.select :close @18:53:10.39 SSV.dial CBV @18:53:10.42
CBV.dial SSV @18:53:11.16 Allowing 130s for 25.0ml of remaining 525.0ml @18:53:11.17 Intake.select :open @18:53:13.32 Intake @15.6psia, vacuum from 10.0 to 13.0psi @18:53:13.34 SS.reconfigure :inhaling 0.78ml/s @18:53:13.38 SS.seek 25ml @18:53:47.94 Stroke completed -- waiting for pressure to equalize ... @18:53:47.95 SS.reconfigure :exhale @18:53:47.99 SS.hold @18:54:00.68 Intake.select :close @18:54:00.82 SSV.dial :exhaust @18:54:01.58 Exhaust.select :open @18:54:01.69 SS.seek 0ml @18:54:10.91 Exhaust.select :close @18:54:11.01 SSV.dial CBV @18:54:11.04
CBV.dial SSV @18:54:11.79 Allowing 130s for 25.0ml of remaining 500.0ml Intake.select :open @18:54:13.94 Intake @15.7psia, vacuum from 10.0 to 13.0psi @18:54:13.96 SS.reconfigure :inhaling 0.78ml/s @18:54:14.00 SS.seek 25ml @18:54:46.15 Vacuum too high -- waiting up to 40 seconds @18:54:46.16 SS.reconfigure :exhale @18:54:46.20 SS.hold @18:54:53.66 Intake @15.1psia, vacuum from 9.6 to 12.6psi @18:54:53.68 SS.reconfigure :inhaling 0.70ml/s @18:54:53.72 SS.seek 25ml @18:54:55.84 Stroke completed -- waiting for pressure to equalize ... @18:54:55.85 SS.reconfigure :exhale @18:54:55.90 SS.hold @18:55:03.26 Intake.select :close @18:55:03.39 SSV.dial :exhaust @18:55:04.14 Exhaust.select :open @18:55:04.25 SS.seek 0ml @18:55:13.02 Exhaust.select :close @18:55:13.12 SSV.dial CBV @18:55:13.14
CBV.dial SSV @18:55:13.89 Allowing 130s for 25.0ml of remaining 475.0ml @18:55:13.90 Intake.select :open @18:55:16.06 Intake @15.9psia, vacuum from 10.0 to 13.0psi @18:55:16.07 SS.reconfigure :inhaling 0.70ml/s @18:55:16.12 SS.seek 25ml @18:55:51.90 Vacuum too high -- waiting up to 40 seconds @18:55:51.91 SS.reconfigure :exhale @18:55:51.95 SS.hold @18:55:56.57 Intake @14.4psia, vacuum from 8.9 to 11.9psi @18:55:56.58 SS.reconfigure :inhaling 0.63ml/s @18:55:56.63 SS.seek 25ml @18:55:59.70 Stroke completed -- waiting for pressure to equalize ... @18:55:59.71 SS.reconfigure :exhale @18:55:59.75 SS.hold @18:56:07.21 Intake.select :close @18:56:07.35 SSV.dial :exhaust @18:56:08.10 Exhaust.select :open @18:56:08.20 SS.seek 0ml @18:56:17.20 Exhaust.select :close @18:56:17.30 SSV.dial CBV @18:56:17.33
CBV.dial SSV @18:56:18.08 Allowing 130s for 25.0ml of remaining 450.0ml @18:56:18.09 Intake.select :open @18:56:20.25 Intake @15.5psia, vacuum from 10.0 to 13.0psi @18:56:20.27 SS.reconfigure :inhaling 0.63ml/s @18:56:20.31 SS.seek 25ml @18:57:01.95 Stroke completed -- waiting for pressure to equalize ... @18:57:01.96 SS.reconfigure :exhale @18:57:02.00 SS.hold @18:57:14.66 Intake.select :close @18:57:14.79 SSV.dial :exhaust @18:57:15.53 Exhaust.select :open @18:57:15.64 SS.seek 0ml @18:57:24.68 Exhaust.select :close @18:57:24.79 SSV.dial CBV @18:57:24.82
CBV.dial SSV @18:57:25.55 Allowing 130s for 25.0ml of remaining 425.0ml @18:57:25.56 Intake.select :open @18:57:27.71 Intake @15.4psia, vacuum from 9.9 to 12.9psi @18:57:27.72 SS.reconfigure :inhaling 0.63ml/s @18:57:27.77 SS.seek 25ml @18:58:09.73 Stroke completed -- waiting for pressure to equalize ... @18:58:09.74 SS.reconfigure :exhale @18:58:09.78 SS.hold @18:58:24.13 Intake.select :close @18:58:24.26 SSV.dial :exhaust @18:58:25.01 Exhaust.select :open @18:58:25.12 SS.seek 0ml @18:58:34.20 Exhaust.select :close @18:58:34.30 SSV.dial CBV @18:58:34.32
CBV.dial SSV @18:58:35.08 Allowing 130s for 25.0ml of remaining 400.0ml @18:58:35.09 Intake.select :open @18:58:37.24 Intake @15.5psia, vacuum from 10.0 to 13.0psi @18:58:37.26 SS.reconfigure :inhaling 0.63ml/s @18:58:37.30 SS.seek 25ml @18:59:18.81 Stroke completed -- waiting for pressure to equalize ... @18:59:18.82 SS.reconfigure :exhale @18:59:18.86 SS.hold @18:59:35.50 Intake.select :close @18:59:35.64 SSV.dial :exhaust @18:59:36.39 Exhaust.select :open @18:59:36.49 SS.seek 0ml @18:59:45.54 Exhaust.select :close @18:59:45.65 SSV.dial CBV @18:59:45.70
CBV.dial SSV @18:59:46.40 Allowing 130s for 25.0ml of remaining 375.0ml @18:59:46.41 Intake.select :open @18:59:48.57 Intake @15.6psia, vacuum from 10.0 to 13.0psi @18:59:48.58 SS.reconfigure :inhaling 0.63ml/s @18:59:48.63 SS.seek 25ml @19:00:27.74 Vacuum too high -- waiting up to 40 seconds @19:00:27.75 SS.reconfigure :exhale @19:00:27.79 SS.hold @19:00:35.25 Intake @14.6psia, vacuum from 9.1 to 12.1psi @19:00:35.27 SS.reconfigure :inhaling 0.57ml/s @19:00:35.31 SS.seek 25ml @19:00:38.18 Stroke completed -- waiting for pressure to equalize ... @19:00:38.19 SS.reconfigure :exhale @19:00:38.23 SS.hold @19:00:48.00 Intake.select :close @19:00:48.14 SSV.dial :exhaust @19:00:48.91 Exhaust.select :open @19:00:49.01 SS.seek 0ml @19:00:58.06 Exhaust.select :close @19:00:58.16 SSV.dial CBV @19:00:58.18
CBV.dial SSV @19:00:58.93 Allowing 130s for 25.0ml of remaining 350.0ml @19:00:58.94 Intake.select :open @19:01:01.10 Intake @15.5psia, vacuum from 10.0 to 13.0psi @19:01:01.12 SS.reconfigure :inhaling 0.57ml/s @19:01:01.16 SS.seek 25ml @19:01:46.74 Stroke completed -- waiting for pressure to equalize ... @19:01:46.75 SS.reconfigure :exhale @19:01:46.79 SS.hold @19:02:05.02 Intake.select :close @19:02:05.16 SSV.dial :exhaust @19:02:05.90 Exhaust.select :open @19:02:06.01 SS.seek 0ml @19:02:15.13 Exhaust.select :close @19:02:15.24 SSV.dial CBV @19:02:15.29
CBV.dial SSV @19:02:15.99 Allowing 130s for 25.0ml of remaining 325.0ml @19:02:16.00 Intake.select :open @19:02:18.16 Intake @15.5psia, vacuum from 10.0 to 13.0psi @19:02:18.18 SS.reconfigure :inhaling 0.57ml/s @19:02:18.22 SS.seek 25ml @19:03:04.07 Stroke completed -- waiting for pressure to equalize ... @19:03:04.08 SS.reconfigure :exhale @19:03:04.12 SS.hold @19:03:22.90 Intake.select :close @19:03:23.04 SSV.dial :exhaust @19:03:23.77 Exhaust.select :open @19:03:23.88 SS.seek 0ml @19:03:32.65 Exhaust.select :close @19:03:32.75 SSV.dial CBV @19:03:32.77
CBV.dial SSV @19:03:33.53 Allowing 130s for 25.0ml of remaining 300.0ml @19:03:33.54 Intake.select :open @19:03:35.69 Intake @15.5psia, vacuum from 10.0 to 13.0psi @19:03:35.71 SS.reconfigure :inhaling 0.57ml/s @19:03:35.75 SS.seek 25ml @19:04:22.08 Stroke completed -- waiting for pressure to equalize ... @19:04:22.09 SS.reconfigure :exhale @19:04:22.14 SS.hold @19:04:44.52 Intake.select :close @19:04:44.66 SSV.dial :exhaust @19:04:45.40 Exhaust.select :open @19:04:45.51 SS.seek 0ml @19:04:54.46 Exhaust.select :close @19:04:54.56 SSV.dial CBV @19:04:54.58
CBV.dial SSV @19:04:55.34 Allowing 130s for 25.0ml of remaining 275.0ml @19:04:55.35 Intake.select :open @19:04:57.50 Intake @15.5psia, vacuum from 10.0 to 13.0psi @19:04:57.51 SS.reconfigure :inhaling 0.57ml/s @19:04:57.56 SS.seek 25ml @19:05:37.27 Vacuum too high -- waiting up to 40 seconds @19:05:37.28 SS.reconfigure :exhale @19:05:37.33 SS.hold @19:05:48.44 Intake @14.9psia, vacuum from 9.4 to 12.4psi @19:05:48.46 SS.reconfigure :inhaling 0.51ml/s @19:05:48.50 SS.seek 25ml @19:05:55.57 Stroke completed -- waiting for pressure to equalize ... SS.reconfigure :exhale @19:05:55.61 SS.hold @19:06:09.86 Intake.select :close @19:06:10.00 SSV.dial :exhaust @19:06:10.74 Exhaust.select :open @19:06:10.84 SS.seek 0ml @19:06:19.78 Exhaust.select :close @19:06:19.89 SSV.dial CBV @19:06:19.94
CBV.dial SSV @19:06:20.74 Allowing 130s for 25.0ml of remaining 250.0ml @19:06:20.75 Intake.select :open @19:06:22.90 Intake @15.5psia, vacuum from 10.0 to 13.0psi @19:06:22.92 SS.reconfigure :inhaling 0.51ml/s @19:06:22.96 SS.seek 25ml @19:07:14.17 Stroke completed -- waiting for pressure to equalize ... @19:07:14.18 SS.reconfigure :exhale @19:07:14.23 SS.hold @19:07:38.67 Intake.select :close @19:07:38.81 SSV.dial :exhaust @19:07:39.55 Exhaust.select :open @19:07:39.66 SS.seek 0ml @19:07:48.44 Exhaust.select :close @19:07:48.54 SSV.dial CBV @19:07:48.56
CBV.dial SSV @19:07:49.32 Allowing 130s for 25.0ml of remaining 225.0ml @19:07:49.33 Intake.select :open @19:07:51.48 Intake @15.6psia, vacuum from 10.0 to 13.0psi @19:07:51.50 SS.reconfigure :inhaling 0.51ml/s @19:07:51.55 SS.seek 25ml @19:08:36.51 Vacuum too high -- waiting up to 40 seconds @19:08:36.52 SS.reconfigure :exhale @19:08:36.56 SS.hold @19:08:49.10 Intake @14.9psia, vacuum from 9.4 to 12.4psi @19:08:49.12 SS.reconfigure :inhaling 0.47ml/s @19:08:49.16 SS.seek 25ml @19:08:56.83 Stroke completed -- waiting for pressure to equalize ... @19:08:56.84 SS.reconfigure :exhale @19:08:56.88 SS.hold @19:09:11.12 Intake.select :close @19:09:11.26 SSV.dial :exhaust @19:09:12.00 Exhaust.select :open @19:09:12.11 SS.seek 0ml @19:09:20.89 Exhaust.select :close @19:09:20.99 SSV.dial CBV @19:09:21.01
CBV.dial SSV @19:09:21.77 Allowing 130s for 25.0ml of remaining 200.0ml @19:09:21.78 Intake.select :open @19:09:23.93 Intake @15.5psia, vacuum from 10.0 to 13.0psi @19:09:23.95 SS.reconfigure :inhaling 0.47ml/s @19:09:23.99 SS.seek 25ml @19:10:21.19 Stroke completed -- waiting for pressure to equalize ... @19:10:21.20 SS.reconfigure :exhale @19:10:21.24 SS.hold @19:10:47.71 Intake.select :close @19:10:47.85 SSV.dial :exhaust @19:10:48.60 Exhaust.select :open @19:10:48.71 SS.seek 0ml @19:10:57.48 Exhaust.select :close @19:10:57.58 SSV.dial CBV @19:10:57.61
CBV.dial SSV @19:10:58.36 Allowing 130s for 25.0ml of remaining 175.0ml @19:10:58.37 Intake.select :open @19:11:00.52 Intake @15.4psia, vacuum from 9.9 to 12.9psi @19:11:00.54 SS.reconfigure :inhaling 0.47ml/s @19:11:00.58 SS.seek 25ml @19:11:47.88 Vacuum too high -- waiting up to 40 seconds @19:11:47.89 SS.reconfigure :exhale @19:11:47.93 SS.hold @19:12:00.49 Intake @15.1psia, vacuum from 9.6 to 12.6psi @19:12:00.51 SS.reconfigure :inhaling 0.43ml/s @19:12:00.55 SS.seek 25ml @19:12:10.78 Stroke completed -- waiting for pressure to equalize ... @19:12:10.79 SS.reconfigure :exhale @19:12:10.83 SS.hold @19:12:29.13 Intake.select :close @19:12:29.27 SSV.dial :exhaust @19:12:30.01 Exhaust.select :open @19:12:30.12 SS.seek 0ml @19:12:39.12 Exhaust.select :close @19:12:39.22 SSV.dial CBV @19:12:39.25
CBV.dial SSV @19:12:40.00 Allowing 130s for 25.0ml of remaining 150.0ml @19:12:40.01 Intake.select :open @19:12:42.16 Intake @15.4psia, vacuum from 9.9 to 12.9psi @19:12:42.18 SS.reconfigure :inhaling 0.43ml/s @19:12:42.22 SS.seek 25ml @19:13:45.32 Stroke completed -- waiting for pressure to equalize ... @19:13:45.33 SS.reconfigure :exhale @19:13:45.37 SS.hold @19:14:13.89 Intake.select :close @19:14:14.02 SSV.dial :exhaust @19:14:14.78 Exhaust.select :open @19:14:14.88 SS.seek 0ml @19:14:23.98 Exhaust.select :close @19:14:24.08 SSV.dial CBV @19:14:24.10
CBV.dial SSV @19:14:25.34 Allowing 130s for 25.0ml of remaining 125.0ml @19:14:25.35 Intake.select :open @19:14:27.50 Intake @15.5psia, vacuum from 10.0 to 13.0psi @19:14:27.52 SS.reconfigure :inhaling 0.43ml/s @19:14:27.56 SS.seek 25ml @19:15:30.46 Stroke completed -- waiting for pressure to equalize ... @19:15:30.47 SS.reconfigure :exhale @19:15:30.51 SS.hold @19:16:03.13 Intake.select :close @19:16:03.27 SSV.dial :exhaust @19:16:04.04 Exhaust.select :open @19:16:04.15 SS.seek 0ml @19:16:13.02 Exhaust.select :close @19:16:13.13 SSV.dial CBV @19:16:13.18
CBV.dial SSV @19:16:13.88 Allowing 130s for 25.0ml of remaining 100.0ml @19:16:13.89 Intake.select :open @19:16:16.05 Intake @15.6psia, vacuum from 10.0 to 13.0psi @19:16:16.07 SS.reconfigure :inhaling 0.43ml/s @19:16:16.11 SS.seek 25ml @19:17:01.84 Vacuum too high -- waiting up to 40 seconds @19:17:01.85 SS.reconfigure :exhale @19:17:01.89 SS.hold @19:17:16.13 Intake @15.4psia, vacuum from 9.9 to 12.9psi @19:17:16.15 SS.reconfigure :inhaling 0.38ml/s @19:17:16.19 SS.seek 25ml @19:17:36.98 Stroke completed -- waiting for pressure to equalize ... @19:17:36.99 SS.reconfigure :exhale @19:17:37.03 SS.hold @19:18:02.32 Intake.select :close @19:18:02.46 SSV.dial :exhaust @19:18:03.21 Exhaust.select :open @19:18:03.32 SS.seek 0ml @19:18:12.27 Exhaust.select :close @19:18:12.37 SSV.dial CBV @19:18:12.39
CBV.dial SSV @19:18:13.15 Allowing 130s for 25.0ml of remaining 74.9ml @19:18:13.16 Intake.select :open @19:18:15.31 Intake @15.7psia, vacuum from 10.0 to 13.0psi @19:18:15.32 SS.reconfigure :inhaling 0.38ml/s @19:18:15.37 SS.seek 25ml @19:19:30.38 Stroke completed -- waiting for pressure to equalize ... @19:19:30.39 SS.reconfigure :exhale @19:19:30.43 SS.hold @19:20:05.47 Intake.select :close @19:20:05.61 SSV.dial :exhaust @19:20:06.35 Exhaust.select :open @19:20:06.45 SS.seek 0ml @19:20:15.28 Exhaust.select :close @19:20:15.39 SSV.dial CBV @19:20:15.42
CBV.dial SSV @19:20:16.16 Allowing 130s for 25.0ml of remaining 49.9ml @19:20:16.17 Intake.select :open @19:20:18.32 Intake @15.7psia, vacuum from 10.0 to 13.0psi @19:20:18.34 SS.reconfigure :inhaling 0.38ml/s @19:20:18.38 SS.seek 25ml @19:21:21.99 Vacuum too high -- waiting up to 40 seconds @19:21:22.00 SS.reconfigure :exhale @19:21:22.04 SS.hold @19:21:38.20 Intake @15.3psia, vacuum from 9.8 to 12.8psi @19:21:38.21 SS.reconfigure :inhaling 0.34ml/s @19:21:38.26 SS.seek 25ml @19:21:52.34 Stroke completed -- waiting for pressure to equalize ... @19:21:52.35 SS.reconfigure :exhale @19:21:52.40 SS.hold @19:22:16.82 Intake.select :close @19:22:16.96 SSV.dial :exhaust @19:22:17.70 Exhaust.select :open @19:22:17.81 SS.seek 0ml @19:22:26.86 Exhaust.select :close @19:22:26.96 SSV.dial CBV @19:22:26.99
CBV.dial SSV @19:22:27.74 Allowing 130s for 24.9ml of remaining 24.9ml @19:22:27.75 Intake.select :open @19:22:29.90 Intake @15.6psia, vacuum from 10.0 to 13.0psi @19:22:29.91 SS.reconfigure :inhaling 0.34ml/s @19:22:29.96 SS.seek 24.95ml @19:23:58.97 Stroke completed -- waiting for pressure to equalize ... SS.reconfigure :exhale @19:23:59.02 SS.hold @19:24:35.62 Intake.select :close @19:24:35.76 SSV.dial :exhaust @19:24:36.50 Exhaust.select :open @19:24:36.61 SS.seek 0ml @19:24:45.42 Exhaust.select :close @19:24:45.52 Sampled 1000.0ml @19:24:45.53 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @19:24:45.54 Intake.select :close @19:24:45.60 CSV.dial :waste @19:24:45.67 CS.seek 0ml @19:24:45.75 SSV.dial CBV,avoiding: :exhaust @19:24:45.77 CSV.dial :air @19:24:45.79
CBV.dial SSV @19:24:46.55 CS.seek 10ml @19:24:47.67
CTV.dial :air @19:24:48.47 delay 15 seconds @19:25:03.49 SSV.dial :spit,avoiding: :exhaust @19:25:04.80 CTV.dial CSV,avoiding: :intake @19:25:04.84
SS.seek 0ml @19:25:04.95 SSV.dial CBV,avoiding: :exhaust @19:25:11.21 CBV.dial between SSV and waste2 @19:25:11.64 CTV.dial CSV,avoiding: :air @19:25:11.66
CSV.dial CTV @19:25:12.24 CS.reconfigure :pressurize @19:25:12.28 CS.seek 0ml @19:25:30.59 BubblePoint: Intake @39.3psia, Outlet @15.0psia @19:25:30.60 CBV.dial SSV @19:25:30.92 SS.reconfigure :default @19:25:30.97 SS.seek 10ml @19:25:36.61 delay 15 seconds @19:25:51.72 Evacuated: Intake @17.0psia, Outlet @9.3psia @19:25:51.73 SSV.dial :spit,avoiding: :exhaust @19:25:53.07 SS.reconfigure :exhale @19:25:53.11 SS.seek 0ml @19:25:58.43 SS.reconfigure :fast @19:25:58.47 SS.seek 5ml @19:26:01.33 SSV.dial CBV,avoiding: :exhaust @19:26:01.35
CBV.dial SSV @19:26:02.69 CTV.dial :air,avoiding: :intake @19:26:04.03 CSV.dial CTV @19:26:04.07
SS.seek 10ml @19:26:05.06 CSV.dial :air @19:26:05.50 CS.reconfigure :fast @19:26:05.55 CS.seek 0ml @19:26:06.55
SSV.dial :spit,avoiding: :exhaust @19:26:07.86 SS.reconfigure :exhale @19:26:07.90 SS.seek 0ml @19:26:12.85 SS.reconfigure :fast @19:26:12.89 SS.seek 5ml @19:26:13.28 CS.stop @19:26:15.69 SSV.dial CBV,avoiding: :exhaust @19:26:15.71
CBV.dial SSV @19:26:18.05 SSV.dial :spit,avoiding: :exhaust @19:26:19.38 SS.reconfigure :default @19:26:19.42 SS.seek 0ml @19:26:22.68 SS.stop @19:26:22.72 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @19:26:22.73 Intake.select :close @19:26:22.79 CSV.dial :waste @19:26:22.90 SSV.dial CBV,avoiding: :exhaust @19:26:22.92
CBV.dial SSV @19:26:23.57 CS.seek 0ml @19:26:23.63 CSV.dial :air @19:26:24.25
CTV.dial :air @19:26:24.26 delay 15 seconds @19:26:24.36 CS.seek 10ml @19:26:39.28
SSV.dial :spit,avoiding: :exhaust @19:26:40.60 CTV.dial CSV,avoiding: :intake @19:26:40.63
SS.reconfigure :exhale @19:26:40.72 SS.seek 0ml @19:26:41.15 SSV.dial CBV,avoiding: :exhaust @19:26:49.05 CBV.dial between SSV and waste2 @19:26:49.44 CTV.dial CSV,avoiding: :air @19:26:49.46
CSV.dial CTV @19:26:49.89 CS.reconfigure :pressurize @19:26:49.93 CS.seek 0ml @19:27:11.65 BubblePoint: Intake @38.1psia, Outlet @14.0psia @19:27:11.66 CBV.dial SSV @19:27:11.98 SS.reconfigure :default @19:27:12.03 SS.seek 10ml @19:27:17.81 delay 15 seconds @19:27:32.93 Evacuated: Intake @18.9psia, Outlet @11.3psia @19:27:32.94 SSV.dial :spit,avoiding: :exhaust @19:27:34.27 SS.reconfigure :exhale @19:27:34.31 SS.seek 0ml @19:27:39.68 SS.reconfigure :fast @19:27:39.72 SS.seek 5ml @19:27:42.30 SSV.dial CBV,avoiding: :exhaust @19:27:42.33
CBV.dial SSV @19:27:43.66 CTV.dial :air,avoiding: :intake @19:27:45.00 CSV.dial CTV @19:27:45.03
SS.seek 10ml @19:27:46.02 CSV.dial :air @19:27:46.47 CS.reconfigure :fast @19:27:46.51 CS.seek 0ml @19:27:47.48
SSV.dial :spit,avoiding: :exhaust @19:27:48.81 SS.reconfigure :exhale @19:27:48.86 SS.seek 0ml @19:27:51.68 CS.stop @19:27:53.74
SS.reconfigure :fast @19:27:53.78 SS.seek 5ml @19:27:56.61 SSV.dial CBV,avoiding: :exhaust @19:27:56.63
CBV.dial SSV @19:27:58.96 SSV.dial :spit,avoiding: :exhaust @19:28:00.27 SS.reconfigure :default @19:28:00.31 SS.seek 0ml @19:28:03.57 SS.stop @19:28:03.61 Lysing: Adding 2.0ml AqMeOH @19:28:03.62 CBV.dial between CSV and waste1,avoiding: :DBV @19:28:05.12 CTV.dial CSV @19:28:06.45 CSV.dial CSR @19:28:07.24 Pulling 1ml of CSR.AqMeOH @19:28:07.25 CSR.select :AqMeOH @19:28:07.36 CS.reconfigure :default @19:28:07.41 CS.seek 1ml @19:28:10.81 delay 5 seconds @19:28:15.82 CSR.select :air @19:28:15.98 CS.seek 6.001ml @19:28:29.62 delay 2 seconds @19:28:31.63 CSV.dial :waste2 @19:28:32.06 CS.reconfigure :fast @19:28:32.11 CS.seek 0ml @19:28:46.62 CSV.dial CSR @19:28:47.12 Pulling 2ml of CSR.AqMeOH @19:28:47.13 CSR.select :AqMeOH @19:28:47.24 CS.reconfigure :default @19:28:47.28 CS.seek 2ml @19:28:53.18 delay 5 seconds @19:28:58.20 CSR.select :air @19:28:58.35 CS.seek 5ml @19:29:06.90 delay 2 seconds @19:29:08.91 CSV.dial CTV @19:29:09.34 CS.seek 1ml @19:29:20.41 Before lysing Collection Puck Heater at 15C/38.9psia @19:29:20.43 Heating Puck.from 2, :sh1 in Collection Clamp to 70C for 10:00 @19:29:20.44 CH.seek 70C,8:00 @19:31:20.33 CH.hold 10:00,70C @19:31:20.49 Begun lysing Collection Puck Heater at 68C/48.3psia @19:41:20.51 Ended lysing Collection Puck Heater at 70C/47.8psia @19:41:20.52 CS.seek 0ml @19:41:23.92 CTV.dial between spare and air,avoiding: :intake @19:41:24.97 CSV.dial :air @19:41:25.41 CS.seek 5ml @19:41:39.17 CSV.dial CTV @19:41:39.62 delay 2 seconds @19:41:41.64 CSV.dial :air @19:41:42.09 delay 2 seconds @19:41:44.11 CSV.dial CTV @19:41:44.56 delay 2 seconds @19:41:46.57 CSV.dial :air @19:41:47.03 delay 2 seconds @19:41:49.05 CS.seek 0ml @19:42:02.69 Puck @77.6psia after final lysis pressurization @19:42:02.70 Recovering Lysate into Collection syringe @19:42:02.71 CSV.dial CBV @19:42:03.71 CBV.dial CSV @19:42:04.07 CS.seek 5ml @19:42:17.92 delay 5 seconds @19:42:22.93 CTV.dial :air @19:42:23.45 CS.seek 7ml @19:42:29.35 delay 5 seconds @19:42:34.37 CSV.dial :air2 @19:42:34.82 CS.seek 10ml @19:42:43.26 CSV.dial between air and CTV,avoiding: CBV @19:42:44.41 Lysing Complete @19:42:44.47 Storing sh1 puck in Collection Clamp to tube 1 @19:42:44.63 SC.reconfigure :tight @19:42:44.68 SC.seek 1 @19:42:48.28 SC.configure :limp @19:42:48.47 Elbow.reconfigure :tight @19:42:48.52 Elbow.seek 1 @19:42:50.28 Forearm.reconfigure :tight @19:42:50.32 Forearm.seek 1 + 450 counts @19:42:51.35 SE.reconfigure :pick @19:42:51.40 SE.seek :beamBreak @19:42:54.04 SE.configure :default @19:42:54.18 Elbow.reconfigure :limp @19:42:54.22 Forearm.reconfigure :loose @19:42:54.27 Forearm.seek 1 @19:42:54.56 Elbow.hold @19:42:54.62 Elbow.reconfigure :tight @19:42:54.72 SE.seek 7.14 Pucks below Top Plate @19:42:55.16 Forearm.force -20 @19:42:55.19 Forearm.seek 1 + 297 counts @19:42:55.50 Forearm.reconfigure :tight @19:42:55.54 Forearm.seek 1 + 450 counts @19:42:55.83 SE.seek 8.14 Pucks below Top Plate @19:42:56.45 Forearm.reconfigure :loose @19:42:56.50 Forearm.seek :retract @19:42:57.44 Elbow.seek CC @19:42:59.13 Elbow.reconfigure :loose @19:42:59.22 Forearm.seek CC @19:43:00.16 Hand.close @19:43:00.83 CC.seek :open @19:43:05.53 Forearm.seek :retract @19:43:06.58 Elbow.reconfigure :tight @19:43:06.62 Elbow.seek 1 @19:43:08.40 Elbow.reconfigure :limp @19:43:08.44 Forearm.seek 1 @19:43:09.40 Elbow.hold @19:43:09.46 Elbow.reconfigure :tight @19:43:09.50 Hand.open @19:43:10.18 Forearm.force -20 @19:43:10.21 Forearm.seek 1 + 310 counts @19:43:10.94 Forearm.reconfigure :tight @19:43:10.99 Forearm.seek 1 + 450 counts @19:43:11.23 SE.seek :down @19:43:12.36
Forearm.reconfigure :loose @19:43:12.41 Forearm.seek :retract @19:43:13.41 SE.stop @19:43:13.46
Tranferring DA @19:43:13.47 Beginning samplePassC @19:43:13.48 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @19:43:13.66 Elbow.seek FlushPuck::Garage @19:43:15.52 Elbow.reconfigure :loose @19:43:15.61 Forearm.seek :garage @19:43:16.59 Hand.close @19:43:17.31 Forearm.seek :retract @19:43:18.34 Elbow.reconfigure :tight @19:43:18.38 Elbow.seek CC @19:43:21.34 Elbow.reconfigure :loose @19:43:21.42 Forearm.reconfigure :tight @19:43:21.47 Forearm.seek CC @19:43:22.65 CC.seek :closed @19:43:27.17 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.28 @19:43:27.23 Hand.open @19:43:27.88 Forearm.reconfigure :loose @19:43:27.92 Forearm.seek :retract @19:43:28.90 PSV.dial PSR @19:43:30.27 Pulling 1.5ml of PSR.flush @19:43:30.28 PSR.select :flush @19:43:30.40 PS.seek 1.5ml @19:43:46.49 delay 5 seconds @19:43:51.50 PSV.dial :waste1 @19:43:51.96 PS.seek 0ml @19:44:08.07 PSV.dial PSR @19:44:08.53 PSR.select :air @19:44:08.64 PS.seek 2.5ml @19:44:35.05 PRV.dial CBV,avoiding: :ab2 @19:44:35.07
CBV.dial PRV @19:44:36.75 PSV.dial :mixloop @19:44:37.78 CTV.dial :air,avoiding: :intake @19:44:37.79 PTV.dial :PRVmixing @19:44:39.11 PS.seek 0ml @19:45:05.60 PSV.dial :air @19:45:06.07 PS.seek 2.5ml @19:45:32.42 PSV.dial :mixloop @19:45:32.88 PS.seek 0ml @19:45:59.26 PSV.dial :air @19:45:59.72 PS.seek 2.5ml @19:46:26.04 PSV.dial :mixloop @19:46:26.50 PS.seek 0ml @19:46:52.92 Garaging FlushPuck in Collection Clamp @19:46:52.99 Elbow.reconfigure :tight @19:46:53.03 Elbow.seek CC @19:46:53.39 Elbow.reconfigure :loose @19:46:53.48 Forearm.seek CC @19:46:54.47 Hand.close @19:46:55.07 CC.seek :open @19:46:59.68 Forearm.seek :retract @19:47:00.67 Elbow.reconfigure :tight @19:47:00.71 Elbow.seek :garage @19:47:03.64 Elbow.reconfigure :loose @19:47:03.73 Forearm.reconfigure :tight @19:47:03.77 Forearm.seek :garage @19:47:04.81 Hand.open @19:47:05.50 Forearm.reconfigure :loose @19:47:05.54 Forearm.seek :retract @19:47:06.55 PSV.dial :mixloop @19:47:06.56 PTV.dial :PRVmixing @19:47:06.58 PRV.dial CBV @19:47:06.60
CBV.dial PRV @19:47:07.07 CTV.dial :air,avoiding: :intake @19:47:07.09 Loading lyfil puck from tube 2 into Collection Clamp @19:47:07.31 SC.reconfigure :tight @19:47:07.35 SC.seek 2 @19:47:10.89 SC.configure :limp @19:47:11.09 Elbow.reconfigure :tight @19:47:11.13 Elbow.seek 2 @19:47:13.07 Forearm.reconfigure :tight @19:47:13.12 Forearm.seek 2 + 450 counts @19:47:14.18 SE.reconfigure :pick @19:47:14.23 SE.seek :beamBreak @19:47:15.79 SE.configure :default @19:47:15.94 Elbow.seek 2 @19:47:16.10 Forearm.seek 2 + 300 counts @19:47:16.46 Forearm.reconfigure :loose @19:47:16.50 Hand.close @19:47:17.08 SE.seek :down @19:47:17.26
Forearm.seek :retract @19:47:18.21 Elbow.seek CC @19:47:18.28 SE.stop @19:47:19.85
Elbow.reconfigure :loose @19:47:19.94 Forearm.reconfigure :tight @19:47:19.98 Forearm.seek CC @19:47:21.18 CC.seek :closed @19:47:25.69 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.10 @19:47:25.70 Hand.open @19:47:26.38 Forearm.reconfigure :loose @19:47:26.43 Forearm.seek :retract @19:47:27.40 CTV.dial CSV,avoiding: :intake @19:47:28.77 CSV.dial CTV @19:47:29.20 PS.seek 2.5ml @19:47:55.79 CS.seek 4.5ml @19:48:10.63 delay 8 seconds @19:48:18.65 PSV.dial :air @19:48:19.12 delay 2 seconds @19:48:21.13 PSV.dial :waste2 @19:48:21.88 PS.seek 0ml @19:48:48.23 CBV.dial between PRV and waste1 @19:48:48.55 CSV.dial :air @19:48:49.00 CS.seek 10ml @19:49:03.97 CSV.dial CTV @19:49:04.42 CS.seek 0ml @19:49:31.03 CBV.dial :waste1 @19:49:31.33 delay 5 seconds @19:49:36.35 CTV.dial :air,avoiding: :intake @19:49:37.75 Storing lyfil puck in Collection Clamp to tube 1 @19:49:37.91 SC.reconfigure :tight @19:49:37.95 SC.seek 1 @19:49:41.51 SC.configure :limp @19:49:41.70 Elbow.reconfigure :tight @19:49:41.74 Elbow.seek 1 @19:49:43.51 Forearm.reconfigure :tight @19:49:43.55 Forearm.seek 1 + 450 counts @19:49:44.55 SE.reconfigure :pick @19:49:44.60 SE.seek :beamBreak @19:49:47.18 SE.configure :default @19:49:47.32 Elbow.reconfigure :limp @19:49:47.37 Forearm.reconfigure :loose @19:49:47.40 Forearm.seek 1 @19:49:47.63 Elbow.hold @19:49:47.69 Elbow.reconfigure :tight @19:49:48.24 SE.seek 8.12 Pucks below Top Plate @19:49:48.68 Forearm.force -20 @19:49:48.72 Forearm.seek 1 + 316 counts @19:49:49.01 Forearm.reconfigure :tight @19:49:49.06 Forearm.seek 1 + 450 counts @19:49:49.25 SE.seek 9.12 Pucks below Top Plate @19:49:49.85 Forearm.reconfigure :loose @19:49:49.89 Forearm.seek :retract @19:49:50.85 Elbow.seek CC @19:49:52.53 Elbow.reconfigure :loose @19:49:52.62 Forearm.seek CC @19:49:53.57 Hand.close @19:49:54.20 CC.seek :open @19:49:58.94 Forearm.seek :retract @19:49:59.98 Elbow.reconfigure :tight @19:50:00.02 Elbow.seek 1 @19:50:01.96 Elbow.reconfigure :limp @19:50:02.01 Forearm.seek 1 @19:50:02.99 Elbow.hold @19:50:03.05 Elbow.reconfigure :tight @19:50:03.09 Hand.open @19:50:03.81 Forearm.force -20 @19:50:03.84 Forearm.seek 1 + 292 counts @19:50:04.13 Forearm.reconfigure :tight @19:50:04.18 Forearm.seek 1 + 450 counts @19:50:04.43 SE.seek :down @19:50:05.55
Forearm.reconfigure :loose @19:50:05.59 Forearm.seek :retract @19:50:06.51 SE.stop @19:50:06.57
PSV.dial :air @19:50:07.33 PS.seek 2.5ml @19:50:33.63 delay 1 second @19:50:34.64 PSV.dial :mixloop @19:50:35.09 delay 1 second @19:50:36.10 PSV.dial :air @19:50:36.55 delay 1 second @19:50:37.57 PSV.dial :mixloop @19:50:38.02 delay 1 second @19:50:39.04 PSV.dial :air @19:50:39.49 delay 1 second @19:50:40.51 PS.seek 0ml @19:51:06.82 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @19:51:06.99 Elbow.seek FlushPuck::Garage @19:51:08.90 Elbow.reconfigure :loose @19:51:08.99 Forearm.seek :garage @19:51:09.96 Hand.close @19:51:10.67 Forearm.seek :retract @19:51:11.65 Elbow.reconfigure :tight @19:51:11.70 Elbow.seek PC @19:51:13.14 Elbow.reconfigure :loose @19:51:13.22 Forearm.reconfigure :tight @19:51:13.26 Forearm.seek PC @19:51:14.52 PC.seek :closed @19:51:19.13 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.56 @19:51:19.19 Hand.open @19:51:19.87 Forearm.reconfigure :loose @19:51:19.92 Forearm.seek :retract @19:51:20.85 PRV.dial :air @19:51:21.30 PSV.dial :mixloop @19:51:21.82 PS.seek 0.3ml @19:51:25.75 PSV.dial PSR @19:51:27.31 Pulling 0.4ml of PSR.air @19:51:27.32 PSR.select :air @19:51:27.35 PS.seek 0.7ml @19:51:32.20 delay 3 seconds @19:51:35.21 PSR.select :air @19:51:35.24 PS.seek 2.5ml @19:51:54.41 delay 3 seconds @19:51:57.43 PSV.dial :mixloop @19:51:58.47 PTV.dial :mixing @19:51:59.24 PBV.dial :waste2 @19:52:00.20 PS.seek 0ml @19:52:26.57 Garaging FlushPuck in Processing Clamp @19:52:26.64 Elbow.reconfigure :tight @19:52:26.68 Elbow.seek PC @19:52:26.79 Elbow.reconfigure :loose @19:52:26.88 Forearm.seek PC @19:52:27.87 Hand.close @19:52:28.44 PC.seek :open @19:52:33.15 Forearm.seek :retract @19:52:34.08 Elbow.reconfigure :tight @19:52:34.13 Elbow.seek :garage @19:52:35.43 Elbow.reconfigure :loose @19:52:35.52 Forearm.reconfigure :tight @19:52:35.56 Forearm.seek :garage @19:52:36.59 Hand.open @19:52:37.25 Forearm.reconfigure :loose @19:52:37.29 Forearm.seek :retract @19:52:38.29 Loading da puck from tube 2 into Processing Clamp @19:52:38.51 SC.reconfigure :tight @19:52:38.56 SC.seek 2 @19:52:42.11 SC.configure :limp @19:52:42.30 Elbow.reconfigure :tight @19:52:42.34 Elbow.seek 2 @19:52:44.17 Forearm.reconfigure :tight @19:52:44.22 Forearm.seek 2 + 450 counts @19:52:45.22 SE.reconfigure :pick @19:52:45.27 SE.seek :beamBreak @19:52:46.97 SE.configure :default @19:52:47.11 Elbow.seek 2 @19:52:47.29 Forearm.seek 2 + 300 counts @19:52:47.74 Forearm.reconfigure :loose @19:52:47.78 Hand.close @19:52:48.45 SE.seek :down @19:52:48.63
Forearm.seek :retract @19:52:49.57 Elbow.seek PC @19:52:49.68 SE.stop @19:52:52.13
Elbow.reconfigure :loose @19:52:52.22 Forearm.reconfigure :tight @19:52:52.27 Forearm.seek PC @19:52:53.42 PC.seek :closed @19:52:58.02 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.61 @19:52:58.03 Hand.open @19:52:58.75 Forearm.reconfigure :loose @19:52:58.79 Forearm.seek :retract @19:52:59.74 Heating Puck.from 2, :da in Processing Clamp to 28C for 2:00:00 @19:52:59.75 PH.seek 28C,5:00 @19:53:37.57 PH.hold 2:00:00,28C @19:53:37.63 delay 1:30 @19:55:07.66 PSR.select :air @19:55:07.67 PSV.dial PSR @19:55:09.25 Pulling 0.5ml of PSR.wash @19:55:09.26 PSR.select :wash @19:55:09.38 PS.seek 0.5ml @19:55:15.32 delay 5 seconds @19:55:20.33 PSR.select :air @19:55:20.45 PS.seek 2ml @19:55:36.66 PTV.dial :puck @19:55:37.38 PSV.dial PTV @19:55:38.14 PBV.dial :waste1 @19:55:39.55 PS.seek 1.5ml @19:55:45.53 delay 3 seconds @19:55:48.55 PBV.dial between PSV and waste1 @19:55:48.92 PS.seek 0ml @19:56:05.00 delay 5:00 @20:01:05.02 recoverPSRreagent08 @20:01:05.03 PTV.dial :puck @20:01:05.04 PBV.dial :waste1 @20:01:05.40 delay 30 seconds @20:01:35.42 PBV.dial between PSV and waste1 @20:01:35.78 PSR.select :air @20:01:35.79 PSV.dial PSR @20:01:36.56 PS.reconfigure :fast @20:01:36.61 PS.seek 2.5ml @20:02:00.62 PSV.dial PTV @20:02:01.37 delay 5 seconds @20:02:06.39 PSV.dial PSR @20:02:07.15 delay 5 seconds @20:02:12.17 PSV.dial PTV @20:02:12.93 delay 5 seconds @20:02:17.95 PSV.dial :air @20:02:18.70 PS.seek 0ml @20:02:41.93 PTV.dial :PRVmixing,via: PRV @20:02:43.24 PSV.dial :mixloop @20:02:43.71 PRV.dial :ab1 @20:02:44.47 PS.reconfigure :slow @20:02:44.51 PS.seek 0.15ml @20:02:48.12 delay 2 seconds @20:02:50.14 CBV.dial PRV @20:02:50.16
PRV.dial CBV @20:02:50.77 PS.seek 0.35ml @20:02:55.43 delay 2 seconds @20:02:57.45 CBV.dial between PRV and PSV @20:02:57.76 PRV.dial :air @20:02:58.20 PS.seek 0.9ml @20:03:09.96 PRV.dial :waste2 @20:03:10.70 PS.seek 0.5ml @20:03:19.37 addPMLReagent08 @20:03:19.38 PSV.dial :air @20:03:19.84 PS.reconfigure :default @20:03:19.88 PS.seek 2ml @20:03:35.99 PSV.dial :PML @20:03:36.44 PTV.dial :mixing @20:03:37.18 PBV.dial :waste1 @20:03:37.58 PS.seek 1.85ml @20:03:39.93 PBV.dial between PSV and waste1 @20:03:40.30 PS.seek 0ml @20:04:00.21 delay 2 seconds @20:04:02.23 Beginning samplePassC @20:04:02.24 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @20:04:02.40 Elbow.reconfigure :tight @20:04:02.45 Elbow.seek FlushPuck::Garage @20:04:03.81 Elbow.reconfigure :loose @20:04:03.91 Forearm.seek :garage @20:04:04.91 Hand.close @20:04:05.67 Forearm.seek :retract @20:04:06.64 Elbow.reconfigure :tight @20:04:06.68 Elbow.seek CC @20:04:09.53 Elbow.reconfigure :loose @20:04:09.62 Forearm.reconfigure :tight @20:04:09.66 Forearm.seek CC @20:04:10.88 CC.seek :closed @20:04:15.39 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.43 @20:04:15.45 Hand.open @20:04:16.12 Forearm.reconfigure :loose @20:04:16.17 Forearm.seek :retract @20:04:17.13 PSV.dial PSR @20:04:18.69 Pulling 1.5ml of PSR.flush PSR.select :flush @20:04:18.81 PS.seek 1.5ml @20:04:34.96 delay 5 seconds @20:04:39.98 PSV.dial :waste1 @20:04:40.44 PS.seek 0ml @20:04:56.52 PSV.dial PSR @20:04:56.99 PSR.select :air @20:04:57.10 PS.seek 2.5ml @20:05:23.47 PRV.dial CBV,avoiding: :ab2 @20:05:23.48
CBV.dial PRV @20:05:25.10 PSV.dial :mixloop @20:05:26.13 CTV.dial :air,avoiding: :intake @20:05:26.14 PTV.dial :PRVmixing @20:05:26.90 PS.seek 0ml @20:05:53.27 PSV.dial :air @20:05:53.84 PS.seek 2.5ml @20:06:20.21 PSV.dial :mixloop @20:06:20.67 PS.seek 0ml @20:06:47.21 PSV.dial :air @20:06:47.67 PS.seek 2.5ml @20:07:13.99 PSV.dial :mixloop @20:07:14.46 PS.seek 0ml @20:07:40.83 Garaging FlushPuck in Collection Clamp @20:07:40.90 Elbow.reconfigure :tight @20:07:40.94 Elbow.seek CC @20:07:41.28 Elbow.reconfigure :loose @20:07:41.37 Forearm.seek CC @20:07:42.35 Hand.close @20:07:42.93 CC.seek :open @20:07:47.53 Forearm.seek :retract @20:07:48.49 Elbow.reconfigure :tight @20:07:48.54 Elbow.seek :garage @20:07:51.51 Elbow.reconfigure :loose @20:07:51.60 Forearm.reconfigure :tight @20:07:51.65 Forearm.seek :garage @20:07:52.65 Hand.open @20:07:53.36 Forearm.reconfigure :loose @20:07:53.41 Forearm.seek :retract @20:07:54.37 delayUntil "20:12:02.22" @20:07:54.40 Whole Cell Archive sampling 1000ml @20:07:54.41 Loading wcr puck from tube 2 into Collection Clamp @20:07:54.69 SC.reconfigure :tight @20:07:54.74 SC.seek 2 @20:07:54.88 SC.configure :limp @20:07:55.08 Elbow.reconfigure :tight @20:07:55.12 Elbow.seek 2 @20:07:57.14 Forearm.reconfigure :tight @20:07:57.18 Forearm.seek 2 + 450 counts @20:07:58.24 SE.reconfigure :pick @20:07:58.29 SE.seek :beamBreak @20:07:59.96 SE.configure :default @20:08:00.10 Elbow.seek 2 @20:08:00.28 Forearm.seek 2 + 300 counts @20:08:00.47 Forearm.reconfigure :loose @20:08:00.52 Hand.close @20:08:01.19 SE.seek :down @20:08:01.37 Forearm.seek :retract @20:08:02.34 Elbow.seek CC @20:08:02.58 SE.stop @20:08:03.95 Elbow.reconfigure :loose @20:08:04.05 Forearm.reconfigure :tight @20:08:04.09 Forearm.seek CC @20:08:05.36 CC.seek :closed @20:08:09.88 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.09 @20:08:09.90 Hand.open @20:08:10.62 Forearm.reconfigure :loose @20:08:10.66 Forearm.seek :retract @20:08:11.65 collectSample 1000ml w/bubble point of 35psi @20:08:11.66 Priming 7ml @20:08:11.67 Intake.select :close @20:08:11.68 Exhaust.select :close @20:08:11.70 SS.hold @20:08:11.89 Calibrating pressure sensors @20:08:11.90 CTV.dial :air @20:08:11.91 SSV.dial :spit,avoiding: :exhaust @20:08:11.93 SS.reconfigure :fast @20:08:11.98 SS.seek 5ml @20:08:14.67 SSV.dial CBV @20:08:14.69 CBV.dial SSV @20:08:16.03 delay 3.8 seconds @20:08:19.96 SSV.dial :spit @20:08:21.28 SS.seek 0ml @20:08:24.07 SSV.dial CBV @20:08:25.41 CTV.dial :intake @20:08:26.18 SSV.dial CBV @20:08:26.20 CBV.dial SSV @20:08:26.21 Allowing 40s for 7.0ml of remaining 7.0ml @20:08:26.22 Intake.select :open @20:08:28.38 Intake @15.1psia, vacuum from 9.6 to 12.6psi @20:08:28.40 SS.reconfigure :inhaling 2.20ml/s @20:08:28.44 SS.seek 7ml @20:08:34.83 Stroke completed -- waiting for pressure to equalize ... @20:08:34.85 SS.reconfigure :exhale @20:08:34.89 SS.hold @20:08:44.58 Intake.select :close @20:08:44.73 SSV.dial :exhaust @20:08:45.49 Exhaust.select :open @20:08:45.60 SS.seek 0ml @20:08:49.93 Exhaust.select :close @20:08:50.04 Sampling 1000.0ml @20:08:50.05 Intake.select :close @20:08:50.06 Exhaust.select :close @20:08:50.07 SS.reconfigure :default @20:08:50.11 SS.hold @20:08:50.30 CTV.dial :intake @20:08:50.32 SSV.dial CBV @20:08:50.35 CBV.dial SSV @20:08:51.09 Allowing 130s for 25.0ml of remaining 1000.0ml @20:08:51.10 Intake.select :open @20:08:53.25 Intake @16.3psia, vacuum from 10.0 to 13.0psi @20:08:53.27 SS.reconfigure :inhaling 2.20ml/s @20:08:53.31 SS.seek 25ml @20:08:58.69 Vacuum too high -- waiting up to 40 seconds @20:08:58.70 SS.reconfigure :exhale @20:08:58.75 SS.hold @20:09:02.04 Intake @16.2psia, vacuum from 10.0 to 13.0psi @20:09:02.05 SS.reconfigure :inhaling 1.98ml/s @20:09:02.10 SS.seek 25ml @20:09:08.16 Vacuum too high -- waiting up to 40 seconds @20:09:08.17 SS.reconfigure :exhale @20:09:08.21 SS.hold @20:09:12.60 Intake @16.6psia, vacuum from 10.0 to 13.0psi @20:09:12.62 SS.reconfigure :inhaling 1.79ml/s @20:09:13.16 SS.seek 25ml @20:09:18.10 Stroke completed -- waiting for pressure to equalize ... @20:09:18.11 SS.reconfigure :exhale @20:09:18.16 SS.hold @20:09:25.67 Intake.select :close @20:09:25.81 SSV.dial :exhaust @20:09:26.55 Exhaust.select :open @20:09:26.66 SS.seek 0ml @20:09:35.82 Exhaust.select :close @20:09:35.93 SSV.dial CBV @20:09:35.96 CBV.dial SSV @20:09:36.70 Allowing 130s for 25.0ml of remaining 975.0ml @20:09:36.71 Intake.select :open @20:09:38.86 Intake @16.8psia, vacuum from 10.0 to 13.0psi @20:09:38.88 SS.reconfigure :inhaling 1.79ml/s @20:09:38.92 SS.seek 25ml @20:09:44.84 Vacuum too high -- waiting up to 40 seconds @20:09:44.85 SS.reconfigure :exhale @20:09:44.90 SS.hold @20:09:46.71 Intake @15.3psia, vacuum from 9.8 to 12.8psi @20:09:46.73 SS.reconfigure :inhaling 1.61ml/s @20:09:46.77 SS.seek 25ml @20:09:59.05 Stroke completed -- waiting for pressure to equalize ... @20:09:59.06 SS.reconfigure :exhale @20:09:59.11 SS.hold @20:10:10.18 Intake.select :close @20:10:10.32 SSV.dial :exhaust @20:10:11.08 Exhaust.select :open @20:10:11.20 SS.seek 0ml @20:10:19.99 Exhaust.select :close @20:10:20.10 SSV.dial CBV @20:10:20.13 CBV.dial SSV @20:10:20.88 Allowing 130s for 25.0ml of remaining 950.0ml @20:10:20.90 Intake.select :open @20:10:23.05 Intake @16.9psia, vacuum from 10.0 to 13.0psi @20:10:23.07 SS.reconfigure :inhaling 1.61ml/s @20:10:23.11 SS.seek 25ml @20:10:30.47 Vacuum too high -- waiting up to 40 seconds @20:10:30.48 SS.reconfigure :exhale @20:10:30.52 SS.hold @20:10:32.72 Intake @16.5psia, vacuum from 10.0 to 13.0psi @20:10:32.74 SS.reconfigure :inhaling 1.45ml/s @20:10:32.78 SS.seek 25ml @20:10:41.87 Vacuum too high -- waiting up to 40 seconds @20:10:41.88 SS.reconfigure :exhale @20:10:41.93 SS.hold @20:10:45.53 Intake @16.6psia, vacuum from 10.0 to 13.0psi @20:10:45.55 SS.reconfigure :inhaling 1.30ml/s @20:10:45.59 SS.seek 25ml @20:10:50.10 Stroke completed -- waiting for pressure to equalize ... @20:10:50.11 SS.reconfigure :exhale @20:10:50.15 SS.hold @20:10:55.52 Intake.select :close @20:10:55.67 SSV.dial :exhaust @20:10:56.43 Exhaust.select :open @20:10:56.54 SS.seek 0ml @20:11:05.14 Exhaust.select :close @20:11:05.25 SSV.dial CBV @20:11:05.28 CBV.dial SSV @20:11:06.02 Allowing 130s for 25.0ml of remaining 925.0ml @20:11:06.04 Intake.select :open @20:11:08.19 Intake @16.8psia, vacuum from 10.0 to 13.0psi @20:11:08.21 SS.reconfigure :inhaling 1.30ml/s @20:11:08.25 SS.seek 25ml @20:11:19.65 Vacuum too high -- waiting up to 40 seconds @20:11:19.67 SS.reconfigure :exhale @20:11:19.71 SS.hold @20:11:22.28 Intake @16.1psia, vacuum from 10.0 to 13.0psi @20:11:22.30 SS.reconfigure :inhaling 1.17ml/s @20:11:22.35 SS.seek 25ml @20:11:35.14 Stroke completed -- waiting for pressure to equalize ... @20:11:35.16 SS.reconfigure :exhale @20:11:35.20 SS.hold @20:11:41.60 Intake.select :close @20:11:41.74 SSV.dial :exhaust @20:11:42.49 Exhaust.select :open @20:11:42.60 SS.seek 0ml @20:11:51.30 Exhaust.select :close @20:11:51.41 SSV.dial CBV @20:11:51.43 CBV.dial SSV @20:11:52.19 Allowing 130s for 25.0ml of remaining 900.0ml @20:11:52.21 Intake.select :open @20:11:54.36 Intake @16.7psia, vacuum from 10.0 to 13.0psi @20:11:54.38 SS.reconfigure :inhaling 1.17ml/s @20:11:54.42 SS.seek 25ml @20:12:02.23 recoverPRVreagent08 @20:12:02.24 PTV.dial :mixing @20:12:03.00 PBV.dial :waste1 @20:12:03.37 delay 30 seconds @20:12:08.09 Vacuum too high -- waiting up to 40 seconds @20:12:08.10 SS.reconfigure :exhale @20:12:08.14 SS.hold @20:12:10.68 Intake @16.2psia, vacuum from 10.0 to 13.0psi @20:12:10.70 SS.reconfigure :inhaling 1.06ml/s @20:12:10.74 SS.seek 25ml @20:12:22.70 Stroke completed -- waiting for pressure to equalize ... @20:12:22.72 SS.reconfigure :exhale @20:12:22.76 SS.hold @20:12:29.17 Intake.select :close @20:12:29.31 SSV.dial :exhaust @20:12:30.07 Exhaust.select :open @20:12:30.18 SS.seek 0ml @20:12:33.39 PBV.dial between PSV and waste1 @20:12:33.77 PSV.dial :air @20:12:34.24 PS.reconfigure :fast @20:12:34.28 PS.seek 2.5ml @20:12:39.10 Exhaust.select :close @20:12:39.20 SSV.dial CBV @20:12:39.23 CBV.dial SSV @20:12:39.99 Allowing 130s for 25.0ml of remaining 875.0ml @20:12:40.00 Intake.select :open @20:12:42.16 Intake @16.7psia, vacuum from 10.0 to 13.0psi @20:12:42.18 SS.reconfigure :inhaling 1.06ml/s @20:12:42.22 SS.seek 25ml @20:12:58.73 PSV.dial :mixloop @20:12:59.20 delay 2 seconds @20:13:01.22 PSV.dial :air @20:13:01.37 Vacuum too high -- waiting up to 40 seconds @20:13:01.38 SS.reconfigure :exhale @20:13:01.43 SS.hold @20:13:01.69 delay 2 seconds @20:13:03.71 PSV.dial :mixloop @20:13:04.17 delay 2 seconds @20:13:04.37 Intake @16.1psia, vacuum from 10.0 to 13.0psi @20:13:04.39 SS.reconfigure :inhaling 0.95ml/s @20:13:04.43 SS.seek 25ml @20:13:06.19 PSV.dial :air @20:13:06.66 PS.seek 0ml @20:13:12.61 Stroke completed -- waiting for pressure to equalize ... @20:13:12.62 SS.reconfigure :exhale @20:13:12.67 SS.hold @20:13:17.43 Intake.select :close @20:13:17.58 SSV.dial :exhaust @20:13:18.33 Exhaust.select :open @20:13:18.44 SS.seek 0ml @20:13:27.44 Exhaust.select :close @20:13:27.55 SSV.dial CBV @20:13:27.57 CBV.dial SSV @20:13:28.35 Allowing 130s for 25.0ml of remaining 850.0ml @20:13:28.36 Intake.select :open @20:13:30.17 washCycleDA #1 of 2 @20:13:30.18 PSR.select :air @20:13:30.20 PSV.dial PSR @20:13:30.51 Intake @16.5psia, vacuum from 10.0 to 13.0psi @20:13:30.53 SS.reconfigure :inhaling 0.95ml/s @20:13:30.57 SS.seek 25ml @20:13:31.57 Pulling 0.5ml of PSR.wash @20:13:31.58 PSR.select :wash @20:13:31.69 PS.reconfigure :default @20:13:31.74 PS.seek 0.5ml @20:13:37.61 delay 5 seconds @20:13:42.63 PSR.select :air @20:13:42.74 PS.seek 2ml @20:13:58.82 PTV.dial :puck @20:13:59.22 Stroke completed -- waiting for pressure to equalize ... @20:13:59.23 SS.reconfigure :exhale @20:13:59.28 SS.hold @20:13:59.56 PSV.dial PTV @20:14:00.31 PBV.dial :waste1 @20:14:00.73 PS.seek 1.5ml @20:14:06.71 delay 3 seconds @20:14:06.78 Intake.select :close @20:14:06.92 SSV.dial :exhaust @20:14:07.69 Exhaust.select :open @20:14:07.80 SS.seek 0ml @20:14:09.73 PBV.dial between PSV and waste1 @20:14:10.11 PS.seek 0ml @20:14:16.64 Exhaust.select :close @20:14:16.75 SSV.dial CBV @20:14:16.77 CBV.dial SSV @20:14:17.53 Allowing 130s for 25.0ml of remaining 825.0ml @20:14:17.55 Intake.select :open @20:14:19.70 Intake @16.4psia, vacuum from 10.0 to 13.0psi @20:14:19.72 SS.reconfigure :inhaling 0.95ml/s @20:14:19.76 SS.seek 25ml @20:14:26.23 delay 2:00 @20:14:48.06 Vacuum too high -- waiting up to 40 seconds @20:14:48.07 SS.reconfigure :exhale @20:14:48.11 SS.hold @20:14:51.99 Intake @15.8psia, vacuum from 10.0 to 13.0psi @20:14:52.01 SS.reconfigure :inhaling 0.87ml/s @20:14:52.06 SS.seek 25ml @20:14:52.92 Stroke completed -- waiting for pressure to equalize ... @20:14:52.93 SS.reconfigure :exhale @20:14:52.98 SS.hold @20:14:56.44 Intake.select :close @20:14:56.58 SSV.dial :exhaust @20:14:57.34 Exhaust.select :open @20:14:57.46 SS.seek 0ml @20:15:06.41 Exhaust.select :close @20:15:06.51 SSV.dial CBV @20:15:06.53 CBV.dial SSV @20:15:07.30 Allowing 130s for 25.0ml of remaining 800.0ml @20:15:07.31 Intake.select :open @20:15:09.47 Intake @16.3psia, vacuum from 10.0 to 13.0psi @20:15:09.49 SS.reconfigure :inhaling 0.87ml/s @20:15:09.53 SS.seek 25ml @20:15:40.69 Stroke completed -- waiting for pressure to equalize ... @20:15:40.70 SS.reconfigure :exhale @20:15:40.74 SS.hold @20:15:48.21 Intake.select :close @20:15:48.35 SSV.dial :exhaust @20:15:49.11 Exhaust.select :open @20:15:49.22 SS.seek 0ml @20:15:57.97 Exhaust.select :close @20:15:58.07 SSV.dial CBV @20:15:58.11 CBV.dial SSV @20:15:58.86 Allowing 130s for 25.0ml of remaining 775.0ml @20:15:58.87 Intake.select :open @20:16:01.03 Intake @16.2psia, vacuum from 10.0 to 13.0psi @20:16:01.05 SS.reconfigure :inhaling 0.87ml/s @20:16:01.09 SS.seek 25ml @20:16:26.25 recoverPSRreagent08 @20:16:26.26 PTV.dial :puck @20:16:26.27 PBV.dial :waste1 @20:16:26.64 delay 30 seconds @20:16:32.48 Stroke completed -- waiting for pressure to equalize ... @20:16:32.49 SS.reconfigure :exhale @20:16:32.54 SS.hold @20:16:40.99 Intake.select :close @20:16:41.14 SSV.dial :exhaust @20:16:41.91 Exhaust.select :open @20:16:42.02 SS.seek 0ml @20:16:50.76 Exhaust.select :close @20:16:50.87 SSV.dial CBV @20:16:50.89 CBV.dial SSV @20:16:51.66 Allowing 130s for 25.0ml of remaining 750.0ml @20:16:51.67 Intake.select :open @20:16:53.82 Intake @16.2psia, vacuum from 10.0 to 13.0psi @20:16:53.84 SS.reconfigure :inhaling 0.87ml/s @20:16:53.88 SS.seek 25ml @20:16:56.65 PBV.dial between PSV and waste1 @20:16:57.03 PSR.select :air @20:16:57.04 PSV.dial PSR @20:16:57.81 PS.reconfigure :fast @20:16:58.34 PS.seek 2.5ml @20:17:22.26 PSV.dial PTV @20:17:23.03 delay 5 seconds @20:17:25.42 Stroke completed -- waiting for pressure to equalize ... @20:17:25.43 SS.reconfigure :exhale @20:17:25.48 SS.hold @20:17:28.04 PSV.dial PSR @20:17:28.79 delay 5 seconds @20:17:33.81 PSV.dial PTV @20:17:34.55 delay 5 seconds @20:17:35.21 Intake.select :close @20:17:35.35 SSV.dial :exhaust @20:17:36.11 Exhaust.select :open @20:17:36.22 SS.seek 0ml @20:17:39.57 PSV.dial :air @20:17:40.34 PS.seek 0ml @20:17:45.36 Exhaust.select :close @20:17:45.47 SSV.dial CBV @20:17:45.49 CBV.dial SSV @20:17:46.25 Allowing 130s for 25.0ml of remaining 725.0ml @20:17:46.26 Intake.select :open @20:17:48.42 Intake @16.2psia, vacuum from 10.0 to 13.0psi @20:17:48.44 SS.reconfigure :inhaling 0.87ml/s @20:17:48.48 SS.seek 25ml @20:18:03.82 washCycleDA #2 of 2 @20:18:03.83 PSR.select :air @20:18:03.85 PSV.dial PSR @20:18:05.22 Pulling 0.5ml of PSR.wash @20:18:05.23 PSR.select :wash @20:18:05.34 PS.reconfigure :default @20:18:05.38 PS.seek 0.5ml @20:18:11.39 delay 5 seconds @20:18:16.40 PSR.select :air @20:18:16.52 PS.seek 2ml @20:18:17.37 Vacuum too high -- waiting up to 40 seconds @20:18:17.38 SS.reconfigure :exhale @20:18:17.42 SS.hold @20:18:23.07 Intake @16.3psia, vacuum from 10.0 to 13.0psi @20:18:23.09 SS.reconfigure :inhaling 0.78ml/s @20:18:23.13 SS.seek 25ml @20:18:25.18 Stroke completed -- waiting for pressure to equalize ... @20:18:25.19 SS.reconfigure :exhale @20:18:25.24 SS.hold @20:18:30.00 Intake.select :close @20:18:30.15 SSV.dial :exhaust @20:18:30.89 Exhaust.select :open @20:18:31.00 SS.seek 0ml @20:18:32.69 PTV.dial :puck @20:18:32.71 PSV.dial PTV @20:18:33.44 PBV.dial :waste1 @20:18:33.88 PS.seek 1.5ml @20:18:39.87 Exhaust.select :close @20:18:39.98 delay 3 seconds @20:18:40.01 SSV.dial CBV @20:18:40.02 CBV.dial SSV @20:18:40.78 Allowing 130s for 25.0ml of remaining 700.0ml @20:18:40.79 Intake.select :open @20:18:42.95 Intake @16.1psia, vacuum from 10.0 to 13.0psi @20:18:42.97 SS.reconfigure :inhaling 0.78ml/s @20:18:43.01 SS.seek 25ml @20:18:43.06 PBV.dial between PSV and waste1 @20:18:43.42 PS.seek 0ml @20:18:59.57 delay 2:00 @20:19:17.24 Stroke completed -- waiting for pressure to equalize ... @20:19:17.26 SS.reconfigure :exhale @20:19:17.30 SS.hold @20:19:26.26 Intake.select :close @20:19:26.40 SSV.dial :exhaust @20:19:27.17 Exhaust.select :open @20:19:27.28 SS.seek 0ml @20:19:35.92 Exhaust.select :close @20:19:36.02 SSV.dial CBV @20:19:36.07 CBV.dial SSV @20:19:36.81 Allowing 130s for 25.0ml of remaining 675.0ml @20:19:36.82 Intake.select :open @20:19:38.98 Intake @15.7psia, vacuum from 10.0 to 13.0psi @20:19:39.00 SS.reconfigure :inhaling 0.78ml/s @20:19:39.04 SS.seek 25ml @20:20:13.82 Stroke completed -- waiting for pressure to equalize ... @20:20:13.83 SS.reconfigure :exhale @20:20:13.88 SS.hold @20:20:24.91 Intake.select :close @20:20:25.05 SSV.dial :exhaust @20:20:25.81 Exhaust.select :open @20:20:25.92 SS.seek 0ml @20:20:35.10 Exhaust.select :close @20:20:35.21 SSV.dial CBV @20:20:35.23 CBV.dial SSV @20:20:36.00 Allowing 130s for 25.0ml of remaining 650.0ml @20:20:36.01 Intake.select :open @20:20:38.16 Intake @16.1psia, vacuum from 10.0 to 13.0psi @20:20:38.18 SS.reconfigure :inhaling 0.78ml/s @20:20:38.22 SS.seek 25ml @20:20:59.60 recoverPSRreagent08 @20:20:59.61 PTV.dial :puck @20:20:59.62 PBV.dial :waste1 @20:20:59.99 delay 30 seconds @20:21:11.85 Stroke completed -- waiting for pressure to equalize ... @20:21:11.86 SS.reconfigure :exhale @20:21:11.91 SS.hold @20:21:24.47 Intake.select :close @20:21:24.62 SSV.dial :exhaust @20:21:25.37 Exhaust.select :open @20:21:25.48 SS.seek 0ml @20:21:30.01 PBV.dial between PSV and waste1 @20:21:30.38 PSR.select :air @20:21:30.40 PSV.dial PSR @20:21:31.17 PS.reconfigure :fast @20:21:31.21 PS.seek 2.5ml @20:21:34.26 Exhaust.select :close @20:21:34.37 SSV.dial CBV @20:21:34.40 CBV.dial SSV @20:21:35.14 Allowing 130s for 25.0ml of remaining 625.0ml @20:21:35.15 Intake.select :open @20:21:37.31 Intake @16.2psia, vacuum from 10.0 to 13.0psi @20:21:37.33 SS.reconfigure :inhaling 0.78ml/s @20:21:37.37 SS.seek 25ml @20:21:55.28 PSV.dial PTV @20:21:56.05 delay 5 seconds @20:22:01.07 PSV.dial PSR @20:22:01.93 Vacuum too high -- waiting up to 40 seconds @20:22:01.94 SS.reconfigure :exhale @20:22:02.00 delay 5 seconds @20:22:02.02 SS.hold @20:22:06.82 Intake @16.1psia, vacuum from 10.0 to 13.0psi @20:22:06.84 SS.reconfigure :inhaling 0.70ml/s @20:22:06.88 SS.seek 25ml @20:22:07.02 PSV.dial PTV @20:22:07.79 delay 5 seconds @20:22:12.80 PSV.dial :air @20:22:13.56 PS.seek 0ml @20:22:18.90 Stroke completed -- waiting for pressure to equalize ... @20:22:18.91 SS.reconfigure :exhale @20:22:18.95 SS.hold @20:22:27.45 Intake.select :close @20:22:27.59 SSV.dial :exhaust @20:22:28.34 Exhaust.select :open @20:22:28.45 SS.seek 0ml @20:22:37.13 getPMLReagent08 @20:22:37.14 PRV.dial :air @20:22:37.23 Exhaust.select :close @20:22:37.33 SSV.dial CBV @20:22:37.36 CBV.dial SSV @20:22:37.60 delay 5 seconds @20:22:38.10 Allowing 130s for 25.0ml of remaining 600.0ml @20:22:38.12 Intake.select :open @20:22:40.27 Intake @16.3psia, vacuum from 10.0 to 13.0psi @20:22:40.29 SS.reconfigure :inhaling 0.70ml/s @20:22:40.33 SS.seek 25ml @20:22:42.62 PTV.dial :PRVmixing @20:22:43.93 delay 5 seconds @20:22:48.94 PRV.dial :ab2 @20:22:49.41 PTV.dial :PRVmixing @20:22:49.42 PSV.dial :mixloop @20:22:49.88 PS.reconfigure :default @20:22:49.93 PS.seek 0.3ml @20:22:53.88 delay 2 seconds @20:22:55.90 PRV.dial :air @20:22:56.42 PS.seek 0.55ml @20:22:59.82 addPMLReagent08 @20:22:59.83 PSV.dial :air @20:23:00.29 PS.seek 2ml @20:23:12.77 Vacuum too high -- waiting up to 40 seconds @20:23:12.78 SS.reconfigure :exhale @20:23:12.82 SS.hold @20:23:15.84 PSV.dial :PML @20:23:16.33 PTV.dial :mixing @20:23:17.08 PBV.dial :waste1 @20:23:17.50 PS.seek 1.85ml @20:23:18.48 Intake @15.9psia, vacuum from 10.0 to 13.0psi @20:23:18.50 SS.reconfigure :inhaling 0.63ml/s @20:23:18.55 SS.seek 25ml @20:23:19.81 PBV.dial between PSV and waste1 @20:23:20.20 PS.seek 0ml @20:23:25.18 Stroke completed -- waiting for pressure to equalize ... @20:23:25.19 SS.reconfigure :exhale @20:23:25.23 SS.hold @20:23:33.11 Intake.select :close @20:23:33.25 SSV.dial :exhaust @20:23:33.99 Exhaust.select :open @20:23:34.10 SS.seek 0ml @20:23:39.85 delay 2 seconds @20:23:41.86 delay 8:00 @20:23:43.21 Exhaust.select :close @20:23:43.32 SSV.dial CBV @20:23:43.34 CBV.dial SSV @20:23:44.10 Allowing 130s for 25.0ml of remaining 575.0ml @20:23:44.12 Intake.select :open @20:23:46.27 Intake @16.2psia, vacuum from 10.0 to 13.0psi @20:23:46.29 SS.reconfigure :inhaling 0.63ml/s @20:23:46.33 SS.seek 25ml @20:24:27.91 Stroke completed -- waiting for pressure to equalize ... @20:24:27.92 SS.reconfigure :exhale @20:24:27.97 SS.hold @20:24:39.01 Intake.select :close @20:24:39.15 SSV.dial :exhaust @20:24:39.91 Exhaust.select :open @20:24:40.02 SS.seek 0ml @20:24:48.85 Exhaust.select :close @20:24:48.96 SSV.dial CBV @20:24:48.99 CBV.dial SSV @20:24:49.74 Allowing 130s for 25.0ml of remaining 550.0ml @20:24:49.75 Intake.select :open @20:24:51.91 Intake @16.2psia, vacuum from 10.0 to 13.0psi @20:24:51.93 SS.reconfigure :inhaling 0.63ml/s @20:24:51.97 SS.seek 25ml @20:25:34.02 Stroke completed -- waiting for pressure to equalize ... @20:25:34.03 SS.reconfigure :exhale @20:25:34.07 SS.hold @20:25:46.74 Intake.select :close @20:25:46.88 SSV.dial :exhaust @20:25:47.63 Exhaust.select :open @20:25:47.74 SS.seek 0ml @20:25:56.55 Exhaust.select :close @20:25:56.66 SSV.dial CBV @20:25:56.68 CBV.dial SSV @20:25:57.44 Allowing 130s for 25.0ml of remaining 525.0ml @20:25:57.46 Intake.select :open @20:25:59.61 Intake @16.0psia, vacuum from 10.0 to 13.0psi @20:25:59.63 SS.reconfigure :inhaling 0.63ml/s @20:25:59.67 SS.seek 25ml @20:26:41.80 Stroke completed -- waiting for pressure to equalize ... @20:26:41.81 SS.reconfigure :exhale @20:26:41.85 SS.hold @20:26:56.70 Intake.select :close @20:26:56.85 SSV.dial :exhaust @20:26:57.60 Exhaust.select :open @20:26:57.71 SS.seek 0ml @20:27:06.75 Exhaust.select :close @20:27:06.86 SSV.dial CBV @20:27:06.88 CBV.dial SSV @20:27:07.65 Allowing 130s for 25.0ml of remaining 500.0ml @20:27:07.66 Intake.select :open @20:27:09.81 Intake @16.3psia, vacuum from 10.0 to 13.0psi @20:27:09.83 SS.reconfigure :inhaling 0.63ml/s @20:27:09.88 SS.seek 25ml @20:27:38.13 Vacuum too high -- waiting up to 40 seconds @20:27:38.15 SS.reconfigure :exhale @20:27:38.19 SS.hold @20:27:43.07 Intake @14.9psia, vacuum from 9.4 to 12.4psi @20:27:43.09 SS.reconfigure :inhaling 0.57ml/s @20:27:43.14 SS.seek 25ml @20:27:58.44 Stroke completed -- waiting for pressure to equalize ... @20:27:58.45 SS.reconfigure :exhale @20:27:58.49 SS.hold @20:28:09.53 Intake.select :close @20:28:09.67 SSV.dial :exhaust @20:28:10.44 Exhaust.select :open @20:28:10.55 SS.seek 0ml @20:28:19.75 Exhaust.select :close @20:28:19.86 SSV.dial CBV @20:28:19.88 CBV.dial SSV @20:28:20.66 Allowing 130s for 25.0ml of remaining 475.0ml @20:28:20.67 Intake.select :open @20:28:22.83 Intake @15.9psia, vacuum from 10.0 to 13.0psi @20:28:22.85 SS.reconfigure :inhaling 0.57ml/s @20:28:22.89 SS.seek 25ml @20:29:08.08 Stroke completed -- waiting for pressure to equalize ... @20:29:08.09 SS.reconfigure :exhale @20:29:08.13 SS.hold @20:29:24.53 Intake.select :close @20:29:24.68 SSV.dial :exhaust @20:29:25.44 Exhaust.select :open @20:29:25.55 SS.seek 0ml @20:29:34.62 Exhaust.select :close @20:29:34.73 SSV.dial CBV @20:29:34.75 CBV.dial SSV @20:29:35.52 Allowing 130s for 25.0ml of remaining 450.0ml @20:29:35.53 Intake.select :open @20:29:37.68 Intake @16.1psia, vacuum from 10.0 to 13.0psi @20:29:37.70 SS.reconfigure :inhaling 0.57ml/s @20:29:37.75 SS.seek 25ml @20:30:12.32 Vacuum too high -- waiting up to 40 seconds @20:30:12.33 SS.reconfigure :exhale @20:30:12.38 SS.hold @20:30:19.40 Intake @15.7psia, vacuum from 10.0 to 13.0psi @20:30:19.41 SS.reconfigure :inhaling 0.51ml/s @20:30:19.46 SS.seek 25ml @20:30:31.79 Stroke completed -- waiting for pressure to equalize ... @20:30:31.81 SS.reconfigure :exhale @20:30:31.85 SS.hold @20:30:44.41 Intake.select :close @20:30:44.55 SSV.dial :exhaust @20:30:45.31 Exhaust.select :open @20:30:45.42 SS.seek 0ml @20:30:54.48 Exhaust.select :close @20:30:54.59 SSV.dial CBV @20:30:54.61 CBV.dial SSV @20:30:55.38 Allowing 130s for 25.0ml of remaining 425.0ml @20:30:55.39 Intake.select :open @20:30:57.54 Intake @16.2psia, vacuum from 10.0 to 13.0psi @20:30:57.56 SS.reconfigure :inhaling 0.51ml/s @20:30:57.60 SS.seek 25ml @20:31:38.97 Vacuum too high -- waiting up to 40 seconds @20:31:38.99 SS.reconfigure :exhale @20:31:39.03 SS.hold @20:31:41.96 recoverPRVreagent08 @20:31:41.97 PTV.dial :mixing @20:31:41.98 PBV.dial :waste1 @20:31:42.34 delay 30 seconds @20:31:45.53 Intake @15.6psia, vacuum from 10.0 to 13.0psi @20:31:45.55 SS.reconfigure :inhaling 0.47ml/s @20:31:45.59 SS.seek 25ml @20:31:55.98 Stroke completed -- waiting for pressure to equalize ... @20:31:55.99 SS.reconfigure :exhale @20:31:56.04 SS.hold @20:32:07.09 Intake.select :close @20:32:07.23 SSV.dial :exhaust @20:32:07.98 Exhaust.select :open @20:32:08.09 SS.seek 0ml @20:32:12.35 PBV.dial between PSV and waste1 @20:32:12.73 PSV.dial :air @20:32:13.20 PS.reconfigure :fast @20:32:13.24 PS.seek 2.5ml @20:32:17.09 Exhaust.select :close @20:32:17.20 SSV.dial CBV @20:32:17.22 CBV.dial SSV @20:32:17.98 Allowing 130s for 25.0ml of remaining 400.0ml @20:32:18.00 Intake.select :open @20:32:20.15 Intake @16.2psia, vacuum from 10.0 to 13.0psi @20:32:20.17 SS.reconfigure :inhaling 0.47ml/s @20:32:20.21 SS.seek 25ml @20:32:37.18 PSV.dial :mixloop @20:32:37.65 delay 2 seconds @20:32:40.09 PSV.dial :air @20:32:40.63 delay 2 seconds @20:32:42.65 PSV.dial :mixloop @20:32:43.12 delay 2 seconds @20:32:45.14 PSV.dial :air @20:32:45.61 PS.seek 0ml @20:33:08.93 washCycleDA #1 of 3 @20:33:08.94 PSR.select :air @20:33:08.96 PSV.dial PSR @20:33:10.33 Pulling 0.5ml of PSR.wash @20:33:10.34 PSR.select :wash @20:33:10.45 PS.reconfigure :default @20:33:10.50 PS.seek 0.5ml @20:33:15.71 Stroke completed -- waiting for pressure to equalize ... @20:33:15.73 SS.reconfigure :exhale @20:33:15.77 SS.hold @20:33:16.49 delay 5 seconds @20:33:21.51 PSR.select :air @20:33:21.62 PS.seek 2ml @20:33:34.04 Intake.select :close @20:33:34.18 SSV.dial :exhaust @20:33:34.92 Exhaust.select :open @20:33:35.03 SS.seek 0ml @20:33:37.83 PTV.dial :puck @20:33:38.59 PSV.dial PTV @20:33:39.33 PBV.dial :waste1 @20:33:39.77 PS.seek 1.5ml @20:33:43.84 Exhaust.select :close @20:33:43.95 SSV.dial CBV @20:33:43.97 CBV.dial SSV @20:33:44.75 Allowing 130s for 25.0ml of remaining 375.0ml @20:33:44.77 Intake.select :open @20:33:45.74 delay 3 seconds @20:33:46.92 Intake @16.1psia, vacuum from 10.0 to 13.0psi @20:33:46.94 SS.reconfigure :inhaling 0.47ml/s @20:33:46.98 SS.seek 25ml @20:33:48.76 PBV.dial between PSV and waste1 @20:33:49.13 PS.seek 0ml @20:34:05.30 delay 2:00 @20:34:35.41 Vacuum too high -- waiting up to 40 seconds @20:34:35.42 SS.reconfigure :exhale @20:34:35.46 SS.hold @20:34:44.04 Intake @16.1psia, vacuum from 10.0 to 13.0psi @20:34:44.06 SS.reconfigure :inhaling 0.43ml/s @20:34:44.10 SS.seek 25ml @20:34:53.12 Stroke completed -- waiting for pressure to equalize ... @20:34:53.13 SS.reconfigure :exhale @20:34:53.17 SS.hold @20:35:04.35 Intake.select :close @20:35:04.49 SSV.dial :exhaust @20:35:05.24 Exhaust.select :open @20:35:05.35 SS.seek 0ml @20:35:14.38 Exhaust.select :close @20:35:15.00 SSV.dial CBV @20:35:15.02 CBV.dial SSV @20:35:15.78 Allowing 130s for 25.0ml of remaining 350.0ml @20:35:15.79 Intake.select :open @20:35:17.95 Intake @16.2psia, vacuum from 10.0 to 13.0psi @20:35:17.96 SS.reconfigure :inhaling 0.43ml/s @20:35:18.01 SS.seek 25ml @20:36:05.33 recoverPSRreagent08 @20:36:05.34 PTV.dial :puck @20:36:05.35 PBV.dial :waste1 @20:36:05.72 delay 30 seconds @20:36:19.99 Stroke completed -- waiting for pressure to equalize ... @20:36:20.00 SS.reconfigure :exhale @20:36:20.05 SS.hold @20:36:35.74 PBV.dial between PSV and waste1 @20:36:36.16 PSR.select :air @20:36:36.17 PSV.dial PSR @20:36:36.93 PS.reconfigure :fast @20:36:36.98 PS.seek 2.5ml @20:36:40.42 Intake.select :close @20:36:40.56 SSV.dial :exhaust @20:36:41.31 Exhaust.select :open @20:36:41.42 SS.seek 0ml @20:36:50.21 Exhaust.select :close @20:36:50.32 SSV.dial CBV @20:36:50.34 CBV.dial SSV @20:36:51.10 Allowing 130s for 25.0ml of remaining 325.0ml @20:36:51.12 Intake.select :open @20:36:53.27 Intake @16.2psia, vacuum from 10.0 to 13.0psi @20:36:53.29 SS.reconfigure :inhaling 0.43ml/s @20:36:53.33 SS.seek 25ml @20:37:00.83 PSV.dial PTV @20:37:01.60 delay 5 seconds @20:37:06.62 PSV.dial PSR @20:37:07.38 delay 5 seconds @20:37:12.40 PSV.dial PTV @20:37:13.16 delay 5 seconds @20:37:18.18 PSV.dial :air @20:37:18.93 PS.seek 0ml @20:37:42.30 washCycleDA #2 of 3 @20:37:42.31 PSR.select :air @20:37:42.33 PSV.dial PSR @20:37:43.70 Pulling 0.5ml of PSR.wash @20:37:43.71 PSR.select :wash @20:37:43.82 PS.reconfigure :default @20:37:43.86 PS.seek 0.5ml @20:37:45.31 Vacuum too high -- waiting up to 40 seconds @20:37:45.33 SS.reconfigure :exhale @20:37:45.37 SS.hold @20:37:49.86 delay 5 seconds @20:37:53.81 Intake @15.5psia, vacuum from 10.0 to 13.0psi @20:37:53.83 SS.reconfigure :inhaling 0.38ml/s @20:37:53.87 SS.seek 25ml @20:37:54.88 PSR.select :air @20:37:54.99 PS.seek 2ml @20:38:05.66 Stroke completed -- waiting for pressure to equalize ... @20:38:05.67 SS.reconfigure :exhale @20:38:05.72 SS.hold @20:38:11.08 PTV.dial :puck @20:38:11.10 PSV.dial PTV @20:38:11.84 PBV.dial :waste1 @20:38:12.27 PS.seek 1.5ml @20:38:18.27 delay 3 seconds @20:38:18.81 Intake.select :close @20:38:18.96 SSV.dial :exhaust @20:38:19.71 Exhaust.select :open @20:38:19.82 SS.seek 0ml @20:38:21.28 PBV.dial between PSV and waste1 @20:38:21.67 PS.seek 0ml @20:38:28.96 Exhaust.select :close @20:38:29.07 SSV.dial CBV @20:38:29.09 CBV.dial SSV @20:38:29.85 Allowing 130s for 25.0ml of remaining 300.0ml @20:38:29.86 Intake.select :open @20:38:32.02 Intake @16.0psia, vacuum from 10.0 to 13.0psi @20:38:32.04 SS.reconfigure :inhaling 0.38ml/s @20:38:32.09 SS.seek 25ml @20:38:37.83 delay 2:00 @20:39:42.67 Stroke completed -- waiting for pressure to equalize ... @20:39:42.69 SS.reconfigure :exhale @20:39:42.73 SS.hold @20:40:03.04 Intake.select :close @20:40:03.18 SSV.dial :exhaust @20:40:03.95 Exhaust.select :open @20:40:04.06 SS.seek 0ml @20:40:12.97 Exhaust.select :close @20:40:13.08 SSV.dial CBV @20:40:13.10 CBV.dial SSV @20:40:13.88 Allowing 130s for 25.0ml of remaining 275.0ml @20:40:13.89 Intake.select :open @20:40:16.05 Intake @16.2psia, vacuum from 10.0 to 13.0psi @20:40:16.07 SS.reconfigure :inhaling 0.38ml/s @20:40:16.12 SS.seek 25ml @20:40:37.86 recoverPSRreagent08 @20:40:37.87 PTV.dial :puck @20:40:37.88 PBV.dial :waste1 @20:40:38.25 delay 30 seconds @20:41:08.28 PBV.dial between PSV and waste1 @20:41:08.62 PSR.select :air @20:41:08.64 PSV.dial PSR @20:41:09.41 PS.reconfigure :fast @20:41:09.45 PS.seek 2.5ml @20:41:16.25 Vacuum too high -- waiting up to 40 seconds @20:41:16.26 SS.reconfigure :exhale @20:41:16.31 SS.hold @20:41:27.32 Intake @16.4psia, vacuum from 10.0 to 13.0psi @20:41:27.34 SS.reconfigure :inhaling 0.34ml/s @20:41:27.38 SS.seek 25ml @20:41:33.39 PSV.dial PTV @20:41:34.16 delay 5 seconds @20:41:39.18 PSV.dial PSR @20:41:39.41 Stroke completed -- waiting for pressure to equalize ... @20:41:39.42 SS.reconfigure :exhale @20:41:39.46 SS.hold @20:41:39.94 delay 5 seconds @20:41:44.96 PSV.dial PTV @20:41:45.70 delay 5 seconds @20:41:50.72 PSV.dial :air @20:41:51.49 PS.seek 0ml @20:41:54.26 Intake.select :close @20:41:54.41 SSV.dial :exhaust @20:41:55.18 Exhaust.select :open @20:41:55.29 SS.seek 0ml @20:42:04.08 Exhaust.select :close @20:42:04.19 SSV.dial CBV @20:42:04.22 CBV.dial SSV @20:42:04.97 Allowing 130s for 25.0ml of remaining 250.0ml @20:42:04.98 Intake.select :open @20:42:07.14 Intake @16.1psia, vacuum from 10.0 to 13.0psi @20:42:07.16 SS.reconfigure :inhaling 0.34ml/s @20:42:07.20 SS.seek 25ml @20:42:14.95 washCycleDA #3 of 3 @20:42:14.96 PSR.select :air @20:42:14.98 PSV.dial PSR @20:42:16.35 Pulling 0.5ml of PSR.wash @20:42:16.36 PSR.select :wash @20:42:16.47 PS.reconfigure :default @20:42:16.51 PS.seek 0.5ml @20:42:22.45 delay 5 seconds @20:42:27.47 PSR.select :air @20:42:27.58 PS.seek 2ml @20:42:43.89 PTV.dial :puck @20:42:43.90 PSV.dial PTV @20:42:44.75 PBV.dial :waste1 @20:42:45.17 PS.seek 1.5ml @20:42:51.14 delay 3 seconds @20:42:54.16 PBV.dial between PSV and waste1 @20:42:54.52 PS.seek 0ml @20:43:10.58 delay 2:00 @20:43:30.17 Stroke completed -- waiting for pressure to equalize ... @20:43:30.18 SS.reconfigure :exhale @20:43:30.23 SS.hold @20:43:52.54 Intake.select :close @20:43:52.68 SSV.dial :exhaust @20:43:53.44 Exhaust.select :open @20:43:53.55 SS.seek 0ml @20:44:02.56 Exhaust.select :close @20:44:02.67 SSV.dial CBV @20:44:02.69 CBV.dial SSV @20:44:03.47 Allowing 130s for 25.0ml of remaining 225.0ml @20:44:03.48 Intake.select :open @20:44:05.64 Intake @16.1psia, vacuum from 10.0 to 13.0psi @20:44:05.66 SS.reconfigure :inhaling 0.34ml/s @20:44:05.70 SS.seek 25ml @20:45:10.60 recoverPSRreagent08 @20:45:10.61 PTV.dial :puck @20:45:10.62 PBV.dial :waste1 @20:45:10.96 delay 30 seconds @20:45:29.86 Stroke completed -- waiting for pressure to equalize ... @20:45:29.87 SS.reconfigure :exhale @20:45:29.91 SS.hold @20:45:40.98 PBV.dial between PSV and waste1 @20:45:41.34 PSR.select :air @20:45:41.36 PSV.dial PSR @20:45:42.11 PS.reconfigure :fast @20:45:42.15 PS.seek 2.5ml @20:45:56.62 Intake.select :close @20:45:56.76 SSV.dial :exhaust @20:45:57.53 Exhaust.select :open @20:45:57.64 SS.seek 0ml @20:46:06.10 PSV.dial PTV @20:46:06.45 Exhaust.select :close @20:46:06.56 SSV.dial CBV @20:46:06.58 CBV.dial SSV @20:46:06.87 delay 5 seconds @20:46:07.36 Allowing 130s for 25.0ml of remaining 200.0ml @20:46:07.37 Intake.select :open @20:46:09.53 Intake @16.2psia, vacuum from 10.0 to 13.0psi @20:46:09.55 SS.reconfigure :inhaling 0.34ml/s @20:46:09.59 SS.seek 25ml @20:46:11.89 PSV.dial PSR @20:46:12.64 delay 5 seconds @20:46:17.66 PSV.dial PTV @20:46:18.42 delay 5 seconds @20:46:23.44 PSV.dial :air @20:46:24.31 PS.seek 0ml @20:46:47.60 Gate.power :camera,:ON @20:46:47.62 Mixing DA Substrates PSR.select :air @20:46:47.64 PSV.dial PSR @20:46:49.17 Pulling 0.25ml of PSR.sub1 @20:46:49.20 PSR.select :sub1 @20:46:49.42 PS.reconfigure :default @20:46:49.54 PS.seek 0.25ml @20:46:52.99 delay 5 seconds @20:46:58.05 Pulling 0.25ml of PSR.sub2 @20:46:58.06 PSR.select :sub2 @20:46:58.18 PS.seek 0.5ml @20:47:01.51 delay 5 seconds @20:47:06.53 PSR.select :air @20:47:06.63 PS.seek 2ml @20:47:22.82 PTV.dial :puck @20:47:22.83 PSV.dial PTV @20:47:23.58 PBV.dial :waste1 @20:47:24.06 PS.seek 1.5ml @20:47:24.08 Vacuum too high -- waiting up to 40 seconds @20:47:24.12 SS.reconfigure :exhale @20:47:24.19 SS.hold @20:47:30.04 delay 3 seconds @20:47:33.05 PBV.dial between PSV and waste1 @20:47:33.44 PS.seek 0ml @20:47:36.87 Intake @15.6psia, vacuum from 10.0 to 13.0psi @20:47:36.89 SS.reconfigure :inhaling 0.31ml/s @20:47:36.93 SS.seek 25ml @20:47:47.87 Stroke completed -- waiting for pressure to equalize ... @20:47:47.88 SS.reconfigure :exhale @20:47:47.92 SS.hold @20:47:49.57 delay 10 seconds @20:47:59.66 recoverPSRreagent08 @20:47:59.67 PTV.dial :puck @20:47:59.68 PBV.dial :waste1 @20:48:00.05 delay 30 seconds @20:48:06.94 Intake.select :close @20:48:07.08 SSV.dial :exhaust @20:48:07.85 Exhaust.select :open @20:48:07.97 SS.seek 0ml @20:48:16.81 Exhaust.select :close @20:48:16.91 SSV.dial CBV @20:48:16.93 CBV.dial SSV @20:48:17.73 Allowing 130s for 25.0ml of remaining 175.0ml @20:48:17.74 Intake.select :open @20:48:19.90 Intake @16.1psia, vacuum from 10.0 to 13.0psi @20:48:19.92 SS.reconfigure :inhaling 0.31ml/s @20:48:19.96 SS.seek 25ml @20:48:30.07 PBV.dial between PSV and waste1 @20:48:30.45 PSR.select :air @20:48:30.46 PSV.dial PSR @20:48:31.23 PS.reconfigure :fast @20:48:31.27 PS.seek 2.5ml @20:48:55.44 PSV.dial PTV @20:48:56.21 delay 5 seconds @20:49:01.23 PSV.dial PSR @20:49:01.98 delay 5 seconds @20:49:07.00 PSV.dial PTV @20:49:07.76 delay 5 seconds @20:49:12.78 PSV.dial :air @20:49:13.55 PS.seek 0ml @20:49:37.19 Additional air clear @20:49:37.20 PSR.select :air @20:49:37.22 PSV.dial PSR @20:49:38.60 Pulling 2ml of PSR.air @20:49:38.61 PSR.select :air @20:49:38.64 PS.reconfigure :default @20:49:38.68 PS.seek 2ml @20:49:53.76 Stroke completed -- waiting for pressure to equalize ... @20:49:53.77 SS.reconfigure :exhale @20:49:53.81 SS.hold @20:49:59.91 delay 5 seconds @20:50:04.92 PTV.dial :puck @20:50:04.94 PSV.dial PTV @20:50:05.68 PBV.dial :waste1 @20:50:06.09 PS.seek 1.5ml @20:50:12.03 delay 3 seconds @20:50:15.05 PBV.dial between PSV and waste1 @20:50:15.43 delay 1 second @20:50:16.45 PSV.dial :waste1 @20:50:17.51 PS.seek 0ml @20:50:24.30 Intake.select :close @20:50:24.44 SSV.dial :exhaust @20:50:25.22 Exhaust.select :open @20:50:25.33 SS.seek 0ml @20:50:33.65 Processing Puck Heater at 28.1C before imaging @20:50:33.66 PH.coast @20:50:33.78 Imaging da puck in Processing Clamp while storing it to tube 1 @20:50:33.94 SC.reconfigure :tight @20:50:33.98 SC.seek 1 @20:50:34.37 Exhaust.select :close @20:50:34.47 SSV.dial CBV @20:50:34.51 CBV.dial SSV @20:50:35.29 Allowing 130s for 25.0ml of remaining 150.0ml @20:50:35.30 Intake.select :open @20:50:37.46 Intake @15.7psia, vacuum from 10.0 to 13.0psi @20:50:37.47 SS.reconfigure :inhaling 0.31ml/s @20:50:37.52 SS.seek 25ml @20:50:37.63 SC.configure :limp @20:50:37.82 Elbow.reconfigure :tight @20:50:37.87 Elbow.seek 1 @20:50:39.63 Forearm.reconfigure :tight @20:50:39.68 Forearm.seek 1 + 450 counts @20:50:40.70 SE.reconfigure :pick @20:50:40.76 SE.seek :beamBreak @20:50:43.24 SE.configure :default @20:50:43.90 Elbow.reconfigure :limp @20:50:43.95 Forearm.reconfigure :loose @20:50:43.99 Forearm.seek 1 @20:50:44.21 Elbow.hold @20:50:44.27 Elbow.reconfigure :tight @20:50:44.36 SE.seek 9.1 Pucks below Top Plate @20:50:44.83 Forearm.force -20 @20:50:44.87 Forearm.seek 1 + 305 counts @20:50:45.17 Forearm.reconfigure :tight @20:50:45.21 Forearm.seek 1 + 450 counts @20:50:45.40 SE.seek 10.1 Pucks below Top Plate @20:50:45.99 Forearm.reconfigure :loose @20:50:46.04 Forearm.seek :retract @20:50:46.96 Elbow.seek PC @20:50:49.72 Elbow.reconfigure :loose @20:50:49.82 Forearm.seek PC @20:50:50.83 Hand.close @20:50:51.40 PC.seek :open @20:50:56.20 Forearm.seek :retract @20:50:57.13 Elbow.reconfigure :tight @20:50:57.18 Elbow.seek 1 @20:50:59.83 Forearm.reconfigure :tight @20:50:59.88 Forearm.seek 1 + 450 counts @20:51:00.92 Hand.open @20:51:01.58 SE.seek 9.75 Pucks below Top Plate @20:51:01.85 SE.reconfigure :seal @20:51:01.89 SE.seek 8.79 Pucks below Top Plate @20:51:02.38 Lights, camera, ACTION! @20:51:02.41 PuckCamera: snap -bin=2,2 -auto /var/log/esp/da22nov1620h1000ml.tif @20:51:20.27 SX HX9/16 (USB:03): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 14.567 seconds /var/log/esp/da22nov1620h1000ml.tif: TIFF Upload Complete @20:51:20.29 PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da22nov1620h1000ml15s.tif @20:51:21.95 Waiting >20 seconds for da to relinquish FlushPuck @20:51:35.91 SX HX9/16 (USB:03): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15 seconds /var/log/esp/midres/da22nov1620h1000ml15s.tif: TIFF Upload Complete @20:51:35.93 PuckCamera: snap -bin=2,2 20.0 /var/log/esp/midres/da22nov1620h1000ml20s.tif @20:51:56.59 SX HX9/16 (USB:03): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 20 seconds /var/log/esp/midres/da22nov1620h1000ml20s.tif: TIFF Upload Complete @20:51:56.61 DAfans.email "14.567 sec auto-exposure",Subject: "da22nov1620h1000ml images!" @20:51:56.64 Gate.power :camera,:OFF @20:51:56.88 Elbow.seek 1 @20:51:57.60 Forearm.seek 1 + 450 counts @20:51:57.84 SE.reconfigure :gently @20:51:57.89 SE.seek 9.51 Pucks below Top Plate @20:51:58.59 Elbow.reconfigure :limp @20:51:58.66 Forearm.reconfigure :loose @20:51:58.70 Forearm.seek 1 @20:51:58.99 Elbow.hold @20:51:59.06 Elbow.reconfigure :tight @20:51:59.11 SE.reconfigure :default @20:51:59.17 SE.seek 9.69 Pucks below Top Plate @20:51:59.54 Forearm.force -20 @20:51:59.58 Forearm.seek 1 + 281 counts @20:51:59.90 Forearm.reconfigure :tight @20:51:59.94 Forearm.seek 1 + 450 counts @20:52:00.20 SE.seek :down @20:52:01.34 Forearm.reconfigure :loose @20:52:01.39 Forearm.seek :retract @20:52:02.28 SE.stop @20:52:02.53 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @20:52:02.93 Elbow.seek FlushPuck::Garage @20:52:04.84 Elbow.reconfigure :loose @20:52:05.05 Forearm.seek :garage @20:52:07.12 Hand.close @20:52:08.02 Forearm.seek :retract @20:52:09.01 Elbow.reconfigure :tight @20:52:09.09 Elbow.seek PC @20:52:10.47 Elbow.reconfigure :loose @20:52:10.66 Forearm.reconfigure :tight @20:52:10.75 Forearm.seek PC @20:52:11.20 Stroke completed -- waiting for pressure to equalize ... @20:52:11.22 SS.reconfigure :exhale @20:52:11.31 SS.hold @20:52:12.90 PC.seek :closed @20:52:17.67 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.58 @20:52:17.82 Hand.open @20:52:18.62 Forearm.reconfigure :loose @20:52:18.71 Forearm.seek :retract @20:52:19.74 PSR.select :air @20:52:19.78 PSV.dial PSR @20:52:20.38 Pulling 0.5ml of PSR.flush @20:52:20.40 PSR.select :flush @20:52:20.59 PS.seek 0.5ml @20:52:26.64 delay 5 seconds @20:52:31.68 PSR.select :air @20:52:31.85 PS.seek 2.5ml @20:52:35.66 upsync @20:52:45.31 SS.reconfigure :default @20:52:45.36 SS.hold @20:52:45.47 Intake.select :close @20:52:45.61 ShallowSampler::Clogged in WCR -- Filtering too Slowly after 875.0ml @20:52:45.62 SSV.dial :exhaust @20:52:46.38 Exhaust.select :open @20:52:46.49 SS.reconfigure :exhale @20:52:46.53 SS.seek 0ml @20:52:53.13 delay 1 second @20:52:54.14 PRV.dial :waste2,avoiding: :ab2 @20:52:55.61 Exhaust.select :close @20:52:55.72 Sampled 875.0ml @20:52:55.74 Drying puck before fixing for 20:00 @20:52:55.75 CBV.dial :waste2b @20:52:56.03 PTV.dial :PRVmixing @20:52:56.51 delay 1:00 @20:52:57.34 PSV.dial :mixloop @20:52:58.39 PS.seek :empty @20:53:24.78 PSR.select :air @20:53:24.80 PSV.dial PSR @20:53:25.88 Pulling 0.5ml of PSR.flush @20:53:25.89 PSR.select :flush @20:53:26.01 PS.seek 0.5ml @20:53:31.97 delay 5 seconds @20:53:36.99 PSR.select :air @20:53:37.10 PS.seek 2.5ml @20:53:56.53 CSV.dial :air @20:53:56.98 CS.reconfigure :fast @20:53:57.03 CS.seek 10ml @20:53:58.38 delay 1 second @20:53:59.39 PBV.dial :waste1 @20:53:59.77 PTV.dial :mixing @20:54:00.51 PSV.dial :mixloop @20:54:01.57 PS.seek :empty @20:54:21.42 Pumping wcr puck from tube 2 up to 35psi... @20:54:21.43 CBV.dial SSV @20:54:22.74 CBV.dial between CSV and waste2b @20:54:24.18 CSV.dial between air and CTV @20:54:24.50 CS.seek 7.141ml @20:54:28.00 PSV.dial PSR @20:54:29.04 PSR.select :flush @20:54:29.16 PS.seek 1ml @20:54:31.86 CTV.dial CSV,avoiding: :air @20:54:31.88 CSV.dial CTV @20:54:32.69 CS.reconfigure :pressurize @20:54:32.73 CS.seek 0ml @20:54:40.15 PSR.select :air @20:54:40.27 PS.seek 2.5ml @20:54:46.37 CBV.dial :waste2b @20:54:46.70 CS.reconfigure :default @20:54:46.74 CS.seek 0ml @20:54:52.57 delay 1:00 @20:54:56.37 PSV.dial PTV @20:54:57.14 PTV.dial :puck @20:54:57.87 PBV.dial :waste1 @20:54:57.89 PS.seek 0ml @20:55:24.22 PSV.dial PSR @20:55:24.97 PSR.select :flush @20:55:25.08 PS.seek 0.5ml @20:55:31.00 PSR.select :air @20:55:31.11 PS.seek 2.5ml @20:55:52.43 PSV.dial PTV @20:55:52.59 CTV.dial :air,avoiding: :intake @20:55:53.20 PBV.dial PSV @20:55:53.77 PS.seek 0ml @20:55:53.92 Fix #1 of 2 (with RNAlater) for 10:00 @20:55:53.93 CSV.dial CSR @20:55:54.42 Pulling 2ml of CSR.RNAlater @20:55:54.43 CSR.select :RNAlater @20:55:54.54 CS.seek 2ml @20:56:00.69 delay 5 seconds @20:56:05.71 CSR.select :air @20:56:05.82 CS.reconfigure :fast @20:56:05.87 CS.seek 10ml @20:56:20.12 PSV.dial PBV @20:56:20.60 PS.seek 2.5ml @20:56:25.51 Pumping wcr puck from tube 2 up to 35psi... @20:56:25.52 CBV.dial SSV @20:56:26.82 CBV.dial between CSV and waste2b @20:56:27.77 CSV.dial between air and CTV @20:56:28.38 CS.seek 7.141ml @20:56:35.64 CTV.dial CSV,avoiding: :air @20:56:35.66 CSV.dial CTV @20:56:37.03 CS.reconfigure :pressurize @20:56:37.07 CS.seek 0ml @20:56:46.90 PSV.dial :waste1 @20:56:47.64 PS.seek 0ml @20:56:53.59 delay 10:00 @20:57:13.93 PSV.dial PSR @20:57:14.40 PSR.select :air @20:57:14.43 PS.seek 2.5ml @20:57:40.78 PSV.dial PTV @20:57:41.51 PBV.dial PSV @20:57:41.53 PS.seek 0ml @20:58:07.84 PSV.dial PBV @20:58:08.31 PS.seek 2.5ml @20:58:34.59 PSV.dial :waste1 @20:58:35.31 PS.seek 0ml @20:59:01.71 PSV.dial PSR @20:59:02.18 PSR.select :flush @20:59:02.30 PS.seek 1ml @20:59:13.62 PSR.select :air @20:59:13.73 PS.seek 2.5ml @20:59:29.90 PSV.dial PTV @20:59:30.63 PTV.dial :puck @20:59:30.65 PBV.dial :waste1 @20:59:31.20 PS.seek 0ml @20:59:57.55 PSV.dial PSR @20:59:58.29 PSR.select :flush @20:59:58.40 PS.seek 0.5ml @21:00:04.74 PSR.select :air @21:00:04.85 PS.seek 2.5ml @21:00:26.09 PSV.dial PTV @21:00:26.96 PBV.dial PSV @21:00:27.53 PS.seek 0ml @21:00:54.08 PSV.dial PBV @21:00:54.55 PS.seek 2.5ml @21:01:20.93 PSV.dial :waste1 @21:01:21.67 PS.seek 0ml @21:01:48.02 PSV.dial PSR @21:01:48.49 PSR.select :air @21:01:48.52 PS.seek 2.5ml @21:02:14.90 PSV.dial PTV @21:02:15.63 PBV.dial PSV @21:02:15.65 PS.seek 0ml @21:02:41.99 PSV.dial PBV @21:02:42.47 PS.seek 2.5ml @21:03:08.86 PSV.dial :waste1 @21:03:09.61 PS.seek 0ml @21:03:35.99 Garaging FlushPuck in Processing Clamp @21:03:36.06 Elbow.reconfigure :tight @21:03:36.11 Elbow.seek PC @21:03:36.22 Elbow.reconfigure :loose @21:03:36.31 Forearm.seek PC @21:03:37.24 Hand.close @21:03:37.89 PC.seek :open @21:03:42.60 Forearm.seek :retract @21:03:43.56 Elbow.reconfigure :tight @21:03:43.60 Elbow.seek :garage @21:03:44.95 Elbow.reconfigure :loose @21:03:45.05 Forearm.reconfigure :tight @21:03:45.09 Forearm.seek :garage @21:03:46.04 Hand.open @21:03:46.69 Forearm.reconfigure :loose @21:03:46.73 Forearm.seek :retract @21:06:53.61 CBV.dial :waste2b @21:06:53.94 CS.reconfigure :default @21:06:53.99 CS.seek 0ml @21:06:56.93 delay 1:00 @21:07:56.96 CTV.dial :air,avoiding: :intake @21:07:58.29 Fix #2 of 2 (with RNAlater) for 10:00 @21:07:58.31 CSV.dial CSR @21:07:58.80 Pulling 2ml of CSR.RNAlater @21:07:58.81 CSR.select :RNAlater @21:07:58.92 CS.seek 2ml @21:08:05.21 delay 5 seconds @21:08:10.24 CSR.select :air @21:08:10.35 CS.reconfigure :fast @21:08:10.39 CS.seek 10ml @21:08:29.87 Pumping wcr puck from tube 2 up to 35psi... @21:08:29.88 CBV.dial SSV @21:08:31.18 CBV.dial between CSV and waste2b @21:08:32.13 CSV.dial between air and CTV @21:08:32.74 CS.seek 7.12ml @21:08:40.13 CTV.dial CSV,avoiding: :air @21:08:40.14 CSV.dial CTV @21:08:41.55 CS.reconfigure :pressurize @21:08:41.59 CS.seek 0ml @21:08:59.51 delay 10:00 @21:18:59.53 CBV.dial :waste2b @21:18:59.87 CS.reconfigure :default @21:18:59.91 CS.seek 0ml @21:19:01.39 delay 1:00 @21:20:01.42 CTV.dial :air,avoiding: :intake @21:20:02.78 Storing wcr puck in Collection Clamp to tube 1 @21:20:03.14 Elbow.reconfigure :tight @21:20:03.19 Elbow.seek 1 @21:20:05.03 Forearm.reconfigure :tight @21:20:05.08 Forearm.seek 1 + 450 counts @21:20:06.60 SE.reconfigure :pick @21:20:06.67 SE.seek :beamBreak @21:20:09.09 SE.configure :default @21:20:09.24 Elbow.reconfigure :limp @21:20:09.29 Forearm.reconfigure :loose @21:20:09.33 Forearm.seek 1 @21:20:09.59 Elbow.hold @21:20:09.66 Elbow.reconfigure :tight @21:20:09.76 SE.seek 10.11 Pucks below Top Plate @21:20:10.22 Forearm.force -20 @21:20:10.26 Forearm.seek 1 + 278 counts @21:20:10.56 Forearm.reconfigure :tight @21:20:10.60 Forearm.seek 1 + 450 counts @21:20:10.81 SE.seek 11.11 Pucks below Top Plate @21:20:11.30 Forearm.reconfigure :loose @21:20:11.35 Forearm.seek :retract @21:20:12.32 Elbow.seek CC @21:20:13.95 Elbow.reconfigure :loose @21:20:14.04 Forearm.seek CC @21:20:14.98 Hand.close @21:20:15.63 CC.seek :open @21:20:20.39 Forearm.seek :retract @21:20:21.35 Elbow.reconfigure :tight @21:20:21.39 Elbow.seek 1 @21:20:23.18 Elbow.reconfigure :limp @21:20:23.28 Forearm.seek 1 @21:20:24.23 Elbow.hold @21:20:24.30 Elbow.reconfigure :tight @21:20:24.35 Hand.open @21:20:25.11 Forearm.force -20 @21:20:25.15 Forearm.seek 1 + 316 counts @21:20:25.45 Forearm.reconfigure :tight @21:20:25.49 Forearm.seek 1 + 450 counts @21:20:25.74 SE.seek :down @21:20:26.86 Forearm.reconfigure :loose @21:20:26.91 Forearm.seek :retract @21:20:27.68 SE.stop @21:20:27.88
Starting finalPurge @21:20:27.89 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @21:20:28.07 Elbow.seek FlushPuck::Garage @21:20:29.90 Elbow.reconfigure :loose @21:20:29.99 Forearm.seek :garage @21:20:30.95 Hand.close @21:20:31.61 Forearm.seek :retract @21:20:32.58 Elbow.reconfigure :tight @21:20:32.62 Elbow.seek CC @21:20:35.57 Elbow.reconfigure :loose @21:20:35.66 Forearm.reconfigure :tight @21:20:35.71 Forearm.seek CC @21:20:36.94 CC.seek :closed @21:20:41.51 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.31 @21:20:41.58 Hand.open @21:20:42.18 Forearm.reconfigure :loose @21:20:42.22 Forearm.seek :retract @21:20:43.25 Flushing the Collection Valve::Manifold @21:20:43.85 CSV.dial CSR @21:20:44.34 Pulling 5ml of CSR.flush CSR.select :flush @21:20:44.45 CS.seek 5ml @21:20:58.02 delay 5 seconds @21:21:03.03 CSR.select :air @21:21:03.18 CS.reconfigure :fast @21:21:03.23 CS.seek 7.5ml @21:21:09.98 CSV.dial :waste @21:21:11.25 CS.reconfigure :default @21:21:11.30 CS.seek 0ml @21:21:31.36 Flushing lower manifold's waste1 port @21:21:31.48 CSV.dial :flush @21:21:31.91 CS.reconfigure :fast @21:21:31.95 CS.seek 5ml @21:21:44.58 delay 5 seconds @21:21:49.60 CSV.dial :air @21:21:50.22 CS.seek 7ml @21:21:55.67 CSV.dial CTV @21:21:55.69
CTV.dial CSV @21:21:57.08 CBV.dial :waste1 @21:21:57.84 CS.seek 0ml @21:22:14.45 Flushing lower manifold's waste2 port @21:22:14.57 CSV.dial :flush @21:22:15.30 CS.seek 5ml @21:22:27.89 delay 5 seconds @21:22:32.91 CSV.dial :air @21:22:33.42 CS.seek 6.999ml @21:22:38.92 CSV.dial CTV @21:22:38.94
CTV.dial CSV @21:22:39.39 CBV.dial :waste2 @21:22:40.44 CS.seek 0ml @21:22:57.02 Flushing lower manifold's waste2b port @21:22:57.13 CSV.dial :flush @21:22:57.87 CS.seek 5ml @21:23:10.45 delay 5 seconds @21:23:15.47 CSV.dial :air @21:23:15.98 CS.seek 7ml @21:23:21.51 CSV.dial CTV @21:23:21.54
CTV.dial CSV @21:23:21.98 CBV.dial :waste2b @21:23:23.02 CS.seek 0ml @21:23:39.48 Disinfecting external Intake with kill @21:23:39.60 CSV.dial CSR @21:23:40.08 Pulling 8ml of CSR.kill @21:23:40.09 CSR.select :kill @21:23:40.20 CS.seek 8ml @21:23:59.92 delay 5 seconds @21:24:04.94 CSR.select :air @21:24:05.05 CS.seek 10ml @21:24:10.61 CSV.dial CBV @21:24:10.63
CBV.dial CSV @21:24:11.68 CTV.dial :intake @21:24:12.45 Intake.select :open @21:24:12.56 CS.seek 2ml @21:24:31.42 Intake.select :close @21:24:31.52 CTV.dial :air @21:24:32.28 CS.reconfigure :default @21:24:32.33 CS.seek 0ml @21:24:38.32 CSV.dial CSR @21:24:39.38 Pulling 5ml of CSR.flush @21:24:39.39 CSR.select :flush @21:24:39.50 CS.seek 5ml @21:24:53.17 delay 5 seconds @21:24:58.19 CSR.select :air @21:24:58.30 CS.seek 10ml @21:25:11.95 CSV.dial CBV @21:25:12.96 CS.seek 0ml @21:25:39.54 Garaging FlushPuck in Collection Clamp @21:25:39.61 Elbow.reconfigure :tight @21:25:39.65 Elbow.seek CC @21:25:40.02 Elbow.reconfigure :loose @21:25:40.11 Forearm.seek CC @21:25:41.06 Hand.close @21:25:41.62 CC.seek :open @21:25:46.24 Forearm.seek :retract @21:25:47.19 Elbow.reconfigure :tight @21:25:47.23 Elbow.seek :garage @21:25:50.19 Elbow.reconfigure :loose @21:25:50.28 Forearm.reconfigure :tight @21:25:50.32 Forearm.seek :garage @21:25:51.36 Hand.open @21:25:52.06 Forearm.reconfigure :loose @21:25:52.10 Forearm.seek :retract @21:25:53.06 Completed [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] begun 18:32:07 @21:25:53.11 Asleep after completing DA_WCR_111622 assays @21:25:53.13 GoodNews.email "{1 => 12,\n 2 => 21,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@21:21:18, 18% flow, 16.3C, 21% humidity, 14.6psia, 13.759V, 0.435A, 0.423A avg, 5.99W\nSampled 875.0ml",Subject: "Asleep after completing DA_WCR_111622 assays" @21:25:58.69 upsync @14:30:56.26UTC22-Nov-22
ESP2 v3.41 I2C Gateway v3.20 for core bus @14:30:58.84 Configuration for short puck jake 1/11/21 by brent@mbari.org /home/esp/esp2/type/shallow/jake/configure.rb @14:31:02.82 Polling Can every 10:00 @14:31:02.87 Loading /home/esp/esp2/mission/DA_WCR_112222.rb mission @14:31:02.92 GoodNews.email "Starting DA_WCR_112222 w/pucks in tube 2\n{}\nCTD data unavailable\nCan@14:31:02, 16% flow, 20.0C, 24% humidity, 14.8psia, 13.808V, 0.260A, 0.242A avg, 3.59W",Subject: "DA_WCR_112222 begun" @14:31:02.95
Preparing to sleep until + 00:00:05 @14:31:02.99 Asleep til + 00:00:05 @14:31:03.01 Continuing at 14:31 @14:31:03.30 Gate.power :core,:OFF @14:31:03.31 Gate.power :raw,:OFF @14:31:03.32 Shutdown @14:31:06.72 GoodNews.email "Then, in tube 2: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2/mission/DA_WCR_112222.rb:14\n{1 => 12,\n 2 => 21,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@14:31:03, 16% flow, 20.0C, 24% humidity, 14.8psia, 13.808V, 0.260A, 0.242A avg, 3.59W",Subject: "Asleep until 14:31" @14:31:07.99
Powering core bus @14:31:08.83 upsync @14:31:12.54 upsync @14:31:13.73
Gate.power :raw,:ON @14:31:14.38 CS.configure :default @14:31:14.47 PS.configure :default @14:31:14.56 SS.configure :default @14:31:14.65 CC.configure :default @14:31:14.74 PC.configure :default @14:31:14.85 Forearm.configure :loose @14:31:14.93 Elbow.configure :loose @14:31:15.04 SC.configure :loose @14:31:15.13 SE.configure :default @14:31:15.17 CH.coast @14:31:15.22 CH.configure :default @14:31:15.25 PH.coast @14:31:15.30 PH.configure :default @14:31:15.33 All dwarf controllers running firmware version 3.85 @14:31:15.44 Valve.reset @14:31:15.65 Intake.select :close @14:31:15.69 Exhaust.select :close @14:31:15.72 CTV.dial :air @14:31:15.83 PSV.dial :air @14:31:15.90 CSV.dial :waste1 @14:31:15.98
Forearm.reconfigure :loose @14:31:16.02 Forearm.home.seek :retract @14:31:16.35 SSV.dial :spit,avoiding: :exhaust @14:31:16.71 Hand.close @14:31:16.77
Elbow.jog 1500 @14:31:16.93 PS.reconfigure :default @14:31:16.98 PS.home.seek 0ml @14:31:17.16 CS.reconfigure :fast @14:31:17.19 SS.reconfigure :default @14:31:17.27 CS.home.seek 0ml @14:31:17.31 SS.home.seek 0ml @14:31:17.41
Elbow.reconfigure :loose @14:31:17.46 Elbow.home.seek :clear @14:31:21.86 SE.reconfigure :default @14:31:21.91 SE.home.seek :down @14:31:22.51 SC.reconfigure :tight @14:31:22.55 SC.home.seek :initial @14:31:25.28 PS.stop @14:31:50.83
SC.configure :limp @14:31:50.88 Forearm.seek :retract @14:31:50.94 Elbow.seek :hammer @14:31:51.98 Forearm.reconfigure :tight @14:31:52.02 Forearm.seek retracted @14:31:52.40 PC.reconfigure :default @14:31:52.42
CC.reconfigure :default @14:31:52.50 PC.home.seek :open @14:31:52.53
CC.home.seek :open @14:31:56.03 Phase [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] @14:31:56.04 Notice.email "At 14:31:55 began in tube 2: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2/mission/DA_WCR_112222.rb:14\n{1 => 12,\n 2 => 21,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@14:31:56, 16% flow, 20.1C, 24% humidity, 14.8psia, 13.788V, 0.315A, 0.296A avg, 4.34W",Subject: "Started DA_WCR_112222 phase initialPurge; da [1000, 1000] #da and wsr are run simultanelously" @14:31:56.06
Starting initialPurge @14:31:56.09 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:31:56.15 Hand.open @14:31:56.92 Elbow.reconfigure :tight @14:31:56.98 Elbow.seek FlushPuck::Garage @14:31:58.88 Elbow.reconfigure :loose @14:31:58.97 Forearm.reconfigure :loose @14:31:59.01 Forearm.seek :garage @14:32:00.03 Hand.close @14:32:00.82 Forearm.seek :retract @14:32:01.53 upsync @14:32:01.78
Elbow.reconfigure :tight @14:32:01.82 Elbow.seek CC @14:32:04.85 Elbow.reconfigure :loose @14:32:04.95 Forearm.reconfigure :tight @14:32:04.99 Forearm.seek CC @14:32:06.24 CC.seek :closed @14:32:10.85 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.20 @14:32:10.92 Hand.open @14:32:11.61 Forearm.reconfigure :loose @14:32:11.66 Forearm.seek :retract @14:32:12.62 Flushing the Collection Valve::Manifold @14:32:12.74 CSV.dial CSR @14:32:14.06 Pulling 5ml of CSR.flush @14:32:14.07 CSR.select :flush @14:32:14.18 CS.reconfigure :default @14:32:14.22 CS.seek 5ml @14:32:27.80 delay 5 seconds @14:32:32.82 CSR.select :air @14:32:32.97 CS.reconfigure :fast @14:32:33.02 CS.seek 7.5ml @14:32:39.74 CSV.dial :waste @14:32:41.01 CS.reconfigure :default @14:32:41.05 CS.seek 0ml @14:33:01.08 Purging external Intake @14:33:01.20 CSV.dial :flush @14:33:01.63 CS.reconfigure :fast @14:33:01.68 CS.seek 10ml @14:33:25.86 delay 5 seconds @14:33:30.88 CSV.dial CBV @14:33:30.92
CBV.dial CSV @14:33:31.63 CTV.dial :intake @14:33:32.42 Intake.select :open @14:33:32.53 CS.seek 0ml @14:33:55.86 Intake.select :close @14:33:55.96 CTV.dial :air @14:33:56.76 Flushing upper manifold @14:33:56.87 CSV.dial :flush @14:33:57.61 CS.seek 5ml @14:34:10.21 delay 5 seconds @14:34:15.22 CSV.dial :air @14:34:15.72 CS.seek 7ml @14:34:21.18 CSV.dial CBV @14:34:21.20
CBV.dial CSV @14:34:22.22 CTV.dial :air @14:34:22.24 CS.seek 0ml @14:34:38.74 delay 7 seconds @14:34:45.76 CS.seek 3ml @14:34:53.70 CSV.dial :waste @14:34:54.16 CS.seek 0ml @14:35:01.90 CSV.dial :flush @14:35:02.34 CS.seek 3ml @14:35:10.24 delay 5 seconds @14:35:15.25 CSV.dial :air @14:35:15.75 CS.seek 5ml @14:35:21.42 CSV.dial :air @14:35:21.44 CS.seek 0ml @14:35:33.61 delay 7 seconds @14:35:40.64 CS.seek 3ml @14:35:48.61 CSV.dial :waste @14:35:49.34 CS.seek 0ml @14:35:56.96 Garaging FlushPuck in Collection Clamp @14:35:57.02 Elbow.reconfigure :tight @14:35:57.07 Elbow.seek CC @14:35:57.17 Elbow.reconfigure :loose @14:35:57.26 Forearm.seek CC @14:35:58.22 Hand.close @14:35:58.79 CC.seek :open @14:36:03.44 Forearm.seek :retract @14:36:04.43 Elbow.reconfigure :tight @14:36:04.47 Elbow.seek :garage @14:36:07.42 Elbow.reconfigure :loose @14:36:07.51 Forearm.reconfigure :tight @14:36:07.55 Forearm.seek :garage @14:36:08.60 Hand.open @14:36:09.72 Forearm.reconfigure :loose @14:36:09.76 Forearm.seek :retract @14:36:10.77 da sampling at most 1000ml, wcr at most 1000ml @14:36:10.81 Loading sh1 puck from tube 2 into Collection Clamp @14:36:10.82 DAfans.email "Started da in tube 2 at 14:36:10UTC22-Nov-22\n{1 => 12,\n 2 => 21,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@14:31:56, 16% flow, 20.1C, 24% humidity, 14.8psia, 13.788V, 0.315A, 0.296A avg, 4.34W",Subject: "da sampling at most 1000ml, wcr at most 1000ml" @14:36:11.08
SC.reconfigure :tight @14:36:11.12 SC.seek 2 @14:36:16.34 upsync @14:36:17.89
SC.configure :limp @14:36:18.10 Elbow.reconfigure :tight @14:36:18.15 Elbow.seek 2 @14:36:20.11 Forearm.reconfigure :tight @14:36:20.15 Forearm.seek 2 + 450 counts @14:36:21.25 SE.reconfigure :pick @14:36:21.30 SE.seek :beamBreak @14:36:23.18 SE.configure :default @14:36:23.33 Elbow.seek 2 @14:36:23.49 Forearm.seek 2 + 300 counts @14:36:23.74 Forearm.reconfigure :loose @14:36:23.78 Hand.close @14:36:24.43 SE.seek :down @14:36:24.62
Forearm.seek :retract @14:36:25.54 Elbow.seek CC @14:36:25.84 SE.stop @14:36:27.09
Elbow.reconfigure :loose @14:36:27.18 Forearm.reconfigure :tight @14:36:27.23 Forearm.seek CC @14:36:28.50 CC.seek :closed @14:36:33.01 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.13 @14:36:33.02 Hand.open @14:36:33.72 Forearm.reconfigure :loose @14:36:33.77 Forearm.seek :retract @14:36:34.70 collectSample 1000ml w/bubble point of 24psi @14:36:34.71 Priming 7ml @14:36:34.72 Intake.select :close @14:36:34.73 Exhaust.select :close @14:36:34.74 SS.hold @14:36:34.93 Calibrating pressure and temperature sensors @14:36:34.96 CTV.dial :air @14:36:34.98 SSV.dial :spit,avoiding: :exhaust @14:36:34.99 SS.reconfigure :fast @14:36:35.04 SS.seek 5ml @14:36:37.94 SSV.dial CBV @14:36:37.96
CBV.dial SSV @14:36:39.28 delay 3.8 seconds @14:36:43.20 SSV.dial :spit @14:36:44.50 SS.seek 0ml @14:36:47.50 SSV.dial CBV @14:36:48.78 CTV.dial :intake @14:36:49.55 SSV.dial CBV @14:36:49.57
CBV.dial SSV @14:36:49.59 Allowing 40s for 7.0ml of remaining 7.0ml @14:36:49.60 Intake.select :open @14:36:51.75 Intake @15.3psia, vacuum from 9.8 to 12.8psi @14:36:51.77 SS.reconfigure :inhaling 2.20ml/s @14:36:51.81 SS.seek 7ml @14:36:57.71 Stroke completed -- waiting for pressure to equalize ... @14:36:57.72 SS.reconfigure :exhale @14:36:57.77 SS.hold @14:36:58.16 Intake.select :close @14:36:58.30 SSV.dial :exhaust @14:36:59.04 Exhaust.select :open @14:36:59.15 SS.seek 0ml @14:37:03.03 Exhaust.select :close @14:37:03.14 Sampling 1000.0ml @14:37:03.15 Intake.select :close @14:37:03.16 Exhaust.select :close @14:37:03.17 SS.reconfigure :default @14:37:03.21 SS.hold @14:37:03.40 CTV.dial :intake @14:37:03.41 SSV.dial CBV @14:37:03.43
CBV.dial SSV @14:37:04.20 Allowing 130s for 25.0ml of remaining 1000.0ml @14:37:04.21 Intake.select :open @14:37:06.37 Intake @14.5psia, vacuum from 9.0 to 12.0psi @14:37:06.38 SS.reconfigure :inhaling 2.20ml/s @14:37:06.42 SS.seek 25ml @14:37:19.21 Vacuum too high -- waiting up to 40 seconds @14:37:19.22 SS.reconfigure :exhale @14:37:19.26 SS.hold @14:37:19.96 Intake @11.2psia, vacuum from 5.7 to 8.7psi @14:37:19.97 SS.reconfigure :inhaling 1.98ml/s @14:37:20.02 SS.seek 25ml @14:37:23.23 Stroke completed -- waiting for pressure to equalize ... @14:37:23.24 SS.reconfigure :exhale @14:37:23.28 SS.hold @14:37:29.06 Intake.select :close @14:37:29.20 SSV.dial :exhaust @14:37:29.95 Exhaust.select :open @14:37:30.06 SS.seek 0ml @14:37:38.95 Exhaust.select :close @14:37:39.05 SSV.dial CBV @14:37:39.07
CBV.dial SSV @14:37:39.84 Allowing 130s for 25.0ml of remaining 975.0ml @14:37:39.85 Intake.select :open @14:37:42.01 Intake @15.2psia, vacuum from 9.7 to 12.7psi @14:37:42.02 SS.reconfigure :inhaling 1.98ml/s @14:37:42.07 SS.seek 25ml @14:37:56.46 Vacuum too high -- waiting up to 40 seconds @14:37:56.47 SS.reconfigure :exhale @14:37:56.51 SS.hold @14:37:57.13 Intake @11.7psia, vacuum from 6.2 to 9.2psi @14:37:57.15 SS.reconfigure :inhaling 1.79ml/s @14:37:57.19 SS.seek 25ml @14:37:59.56 Stroke completed -- waiting for pressure to equalize ... @14:37:59.57 SS.reconfigure :exhale @14:37:59.61 SS.hold @14:38:06.14 Intake.select :close @14:38:06.28 SSV.dial :exhaust @14:38:07.03 Exhaust.select :open @14:38:07.13 SS.seek 0ml @14:38:15.92 Exhaust.select :close @14:38:16.02 SSV.dial CBV @14:38:16.05
CBV.dial SSV @14:38:16.79 Allowing 130s for 25.0ml of remaining 950.0ml @14:38:16.80 Intake.select :open @14:38:18.96 Intake @15.3psia, vacuum from 9.8 to 12.8psi @14:38:18.97 SS.reconfigure :inhaling 1.79ml/s @14:38:19.02 SS.seek 25ml @14:38:36.62 Stroke completed -- waiting for pressure to equalize ... @14:38:36.63 SS.reconfigure :exhale @14:38:36.67 SS.hold @14:38:44.16 Intake.select :close @14:38:44.29 SSV.dial :exhaust @14:38:45.04 Exhaust.select :open @14:38:45.15 SS.seek 0ml @14:38:54.21 Exhaust.select :close @14:38:54.32 SSV.dial CBV @14:38:54.34
CBV.dial SSV @14:38:55.09 Allowing 130s for 25.0ml of remaining 925.0ml @14:38:55.10 Intake.select :open @14:38:57.25 Intake @15.4psia, vacuum from 9.9 to 12.9psi @14:38:57.27 SS.reconfigure :inhaling 1.79ml/s @14:38:57.31 SS.seek 25ml @14:39:13.64 Vacuum too high -- waiting up to 40 seconds @14:39:13.65 SS.reconfigure :exhale @14:39:13.69 SS.hold @14:39:14.27 Intake @12.0psia, vacuum from 6.5 to 9.5psi @14:39:14.29 SS.reconfigure :inhaling 1.61ml/s @14:39:14.33 SS.seek 25ml @14:39:14.58 Vacuum too high -- waiting up to 40 seconds @14:39:14.59 SS.reconfigure :exhale @14:39:14.63 SS.hold @14:39:21.02 Intake @13.6psia, vacuum from 8.1 to 11.1psi @14:39:21.03 SS.reconfigure :inhaling 1.45ml/s @14:39:21.08 SS.seek 25ml @14:39:22.24 Stroke completed -- waiting for pressure to equalize ... @14:39:22.25 SS.reconfigure :exhale @14:39:22.29 SS.hold @14:39:22.59 Intake.select :close @14:39:22.73 SSV.dial :exhaust @14:39:23.46 Exhaust.select :open @14:39:23.57 SS.seek 0ml @14:39:32.59 Exhaust.select :close @14:39:32.69 SSV.dial CBV @14:39:32.71
CBV.dial SSV @14:39:33.46 Allowing 130s for 25.0ml of remaining 900.0ml @14:39:33.47 Intake.select :open @14:39:35.63 Intake @15.2psia, vacuum from 9.7 to 12.7psi @14:39:35.65 SS.reconfigure :inhaling 1.45ml/s @14:39:35.69 SS.seek 25ml @14:39:55.66 Stroke completed -- waiting for pressure to equalize ... @14:39:55.67 SS.reconfigure :exhale @14:39:55.71 SS.hold @14:40:02.70 Intake.select :close @14:40:02.84 SSV.dial :exhaust @14:40:03.59 Exhaust.select :open @14:40:03.69 SS.seek 0ml @14:40:12.69 Exhaust.select :close @14:40:12.80 SSV.dial CBV @14:40:12.82
CBV.dial SSV @14:40:13.57 Allowing 130s for 25.0ml of remaining 875.0ml @14:40:13.58 Intake.select :open @14:40:15.74 Intake @15.3psia, vacuum from 9.8 to 12.8psi @14:40:15.76 SS.reconfigure :inhaling 1.45ml/s @14:40:15.80 SS.seek 25ml @14:40:35.85 Stroke completed -- waiting for pressure to equalize ... @14:40:35.86 SS.reconfigure :exhale @14:40:35.90 SS.hold @14:40:43.37 Intake.select :close @14:40:43.51 SSV.dial :exhaust @14:40:44.26 Exhaust.select :open @14:40:44.36 SS.seek 0ml @14:40:53.19 Exhaust.select :close @14:40:53.30 SSV.dial CBV @14:40:53.33
CBV.dial SSV @14:40:54.07 Allowing 130s for 25.0ml of remaining 850.0ml @14:40:54.08 Intake.select :open @14:40:56.23 Intake @15.3psia, vacuum from 9.8 to 12.8psi @14:40:56.25 SS.reconfigure :inhaling 1.45ml/s @14:40:56.29 SS.seek 25ml @14:41:17.29 Stroke completed -- waiting for pressure to equalize ... @14:41:17.30 SS.reconfigure :exhale @14:41:17.34 SS.hold @14:41:24.79 Intake.select :close @14:41:24.93 SSV.dial :exhaust @14:41:25.68 Exhaust.select :open @14:41:25.78 SS.seek 0ml @14:41:34.80 Exhaust.select :close @14:41:34.90 SSV.dial CBV @14:41:34.94
CBV.dial SSV @14:41:35.68 Allowing 130s for 25.0ml of remaining 825.0ml @14:41:35.69 Intake.select :open @14:41:37.84 Intake @15.2psia, vacuum from 9.7 to 12.7psi @14:41:37.86 SS.reconfigure :inhaling 1.45ml/s @14:41:37.90 SS.seek 25ml @14:41:58.29 Stroke completed -- waiting for pressure to equalize ... @14:41:58.30 SS.reconfigure :exhale @14:41:58.34 SS.hold @14:42:06.87 Intake.select :close @14:42:07.01 SSV.dial :exhaust @14:42:07.76 Exhaust.select :open @14:42:07.86 SS.seek 0ml @14:42:16.89 Exhaust.select :close @14:42:17.00 SSV.dial CBV @14:42:17.02
CBV.dial SSV @14:42:17.77 Allowing 130s for 25.0ml of remaining 800.0ml @14:42:17.78 Intake.select :open @14:42:19.94 Intake @15.3psia, vacuum from 9.8 to 12.8psi @14:42:19.95 SS.reconfigure :inhaling 1.45ml/s @14:42:20.00 SS.seek 25ml @14:42:39.26 Vacuum too high -- waiting up to 40 seconds @14:42:39.27 SS.reconfigure :exhale @14:42:39.31 SS.hold @14:42:42.99 Intake @13.2psia, vacuum from 7.7 to 10.7psi @14:42:43.00 SS.reconfigure :inhaling 1.30ml/s @14:42:43.04 SS.seek 25ml @14:42:43.92 Stroke completed -- waiting for pressure to equalize ... @14:42:43.93 SS.reconfigure :exhale @14:42:43.98 SS.hold @14:42:48.82 Intake.select :close @14:42:48.96 SSV.dial :exhaust @14:42:49.69 Exhaust.select :open @14:42:49.80 SS.seek 0ml @14:42:58.60 Exhaust.select :close @14:42:58.70 SSV.dial CBV @14:42:58.72
CBV.dial SSV @14:42:59.47 Allowing 130s for 25.0ml of remaining 775.0ml @14:42:59.48 Intake.select :open @14:43:01.64 Intake @15.3psia, vacuum from 9.8 to 12.8psi @14:43:01.65 SS.reconfigure :inhaling 1.30ml/s @14:43:01.69 SS.seek 25ml @14:43:24.08 Stroke completed -- waiting for pressure to equalize ... @14:43:24.09 SS.reconfigure :exhale @14:43:24.61 SS.hold @14:43:32.99 Intake.select :close @14:43:33.13 SSV.dial :exhaust @14:43:33.86 Exhaust.select :open @14:43:33.97 SS.seek 0ml @14:43:42.75 Exhaust.select :close @14:43:42.86 SSV.dial CBV @14:43:42.91
CBV.dial SSV @14:43:43.61 Allowing 130s for 25.0ml of remaining 750.0ml @14:43:43.62 Intake.select :open @14:43:45.78 Intake @15.4psia, vacuum from 9.9 to 12.9psi @14:43:45.79 SS.reconfigure :inhaling 1.30ml/s @14:43:45.84 SS.seek 25ml @14:44:08.25 Stroke completed -- waiting for pressure to equalize ... @14:44:08.26 SS.reconfigure :exhale @14:44:08.30 SS.hold @14:44:18.06 Intake.select :close @14:44:18.19 SSV.dial :exhaust @14:44:18.94 Exhaust.select :open @14:44:19.05 SS.seek 0ml @14:44:27.86 Exhaust.select :close @14:44:27.96 SSV.dial CBV @14:44:27.99
CBV.dial SSV @14:44:28.74 Allowing 130s for 25.0ml of remaining 725.0ml @14:44:28.75 Intake.select :open @14:44:30.90 Intake @15.3psia, vacuum from 9.8 to 12.8psi @14:44:30.92 SS.reconfigure :inhaling 1.30ml/s @14:44:30.96 SS.seek 25ml @14:44:50.85 Vacuum too high -- waiting up to 40 seconds @14:44:50.86 SS.reconfigure :exhale @14:44:50.90 SS.hold @14:44:55.11 Intake @13.7psia, vacuum from 8.2 to 11.2psi @14:44:55.13 SS.reconfigure :inhaling 1.17ml/s @14:44:55.17 SS.seek 25ml @14:44:58.77 Stroke completed -- waiting for pressure to equalize ... SS.reconfigure :exhale @14:44:58.81 SS.hold @14:45:03.59 Intake.select :close @14:45:03.72 SSV.dial :exhaust @14:45:04.47 Exhaust.select :open @14:45:04.58 SS.seek 0ml @14:45:13.36 Exhaust.select :close @14:45:13.46 SSV.dial CBV @14:45:13.48
CBV.dial SSV @14:45:14.24 Allowing 130s for 25.0ml of remaining 700.0ml @14:45:14.25 Intake.select :open @14:45:16.40 Intake @15.3psia, vacuum from 9.8 to 12.8psi @14:45:16.42 SS.reconfigure :inhaling 1.17ml/s @14:45:16.46 SS.seek 25ml @14:45:40.94 Stroke completed -- waiting for pressure to equalize ... @14:45:40.95 SS.reconfigure :exhale @14:45:40.99 SS.hold @14:45:50.65 Intake.select :close @14:45:50.78 SSV.dial :exhaust @14:45:51.53 Exhaust.select :open @14:45:51.64 SS.seek 0ml @14:46:00.69 Exhaust.select :close @14:46:00.79 SSV.dial CBV @14:46:00.81
CBV.dial SSV @14:46:01.56 Allowing 130s for 25.0ml of remaining 675.0ml @14:46:01.57 Intake.select :open @14:46:03.73 Intake @15.3psia, vacuum from 9.8 to 12.8psi @14:46:03.74 SS.reconfigure :inhaling 1.17ml/s @14:46:03.79 SS.seek 25ml @14:46:27.04 Vacuum too high -- waiting up to 40 seconds @14:46:27.05 SS.reconfigure :exhale @14:46:27.09 SS.hold @14:46:32.45 Intake @13.8psia, vacuum from 8.3 to 11.3psi @14:46:32.46 SS.reconfigure :inhaling 1.06ml/s @14:46:32.51 SS.seek 25ml @14:46:33.40 Stroke completed -- waiting for pressure to equalize ... @14:46:33.41 SS.reconfigure :exhale @14:46:33.46 SS.hold @14:46:38.33 Intake.select :close @14:46:38.47 SSV.dial :exhaust @14:46:39.20 Exhaust.select :open @14:46:39.31 SS.seek 0ml @14:46:48.12 Exhaust.select :close @14:46:48.23 SSV.dial CBV @14:46:48.25
CBV.dial SSV @14:46:49.00 Allowing 130s for 25.0ml of remaining 650.0ml @14:46:49.01 Intake.select :open @14:46:51.16 Intake @15.3psia, vacuum from 9.8 to 12.8psi @14:46:51.18 SS.reconfigure :inhaling 1.06ml/s @14:46:51.22 SS.seek 25ml @14:47:17.87 Stroke completed -- waiting for pressure to equalize ... @14:47:17.88 SS.reconfigure :exhale @14:47:17.92 SS.hold @14:47:27.68 Intake.select :close @14:47:27.82 SSV.dial :exhaust @14:47:28.57 Exhaust.select :open @14:47:28.67 SS.seek 0ml @14:47:37.53 Exhaust.select :close @14:47:37.63 SSV.dial CBV @14:47:37.65
CBV.dial SSV @14:47:38.41 Allowing 130s for 25.0ml of remaining 625.0ml @14:47:38.42 Intake.select :open @14:47:40.57 Intake @15.3psia, vacuum from 9.8 to 12.8psi @14:47:40.59 SS.reconfigure :inhaling 1.06ml/s @14:47:40.63 SS.seek 25ml @14:48:04.22 Vacuum too high -- waiting up to 40 seconds @14:48:04.23 SS.reconfigure :exhale @14:48:04.28 SS.hold @14:48:08.44 Intake @13.6psia, vacuum from 8.1 to 11.1psi @14:48:08.45 SS.reconfigure :inhaling 0.95ml/s @14:48:08.49 SS.seek 25ml @14:48:12.31 Stroke completed -- waiting for pressure to equalize ... @14:48:12.32 SS.reconfigure :exhale @14:48:12.36 SS.hold @14:48:18.86 Intake.select :close @14:48:18.99 SSV.dial :exhaust @14:48:19.73 Exhaust.select :open @14:48:19.84 SS.seek 0ml @14:48:28.56 Exhaust.select :close @14:48:28.66 SSV.dial CBV @14:48:28.68
CBV.dial SSV @14:48:29.44 Allowing 130s for 25.0ml of remaining 600.0ml @14:48:29.45 Intake.select :open @14:48:31.60 Intake @15.2psia, vacuum from 9.7 to 12.7psi @14:48:31.62 SS.reconfigure :inhaling 0.95ml/s @14:48:31.66 SS.seek 25ml @14:49:00.73 Stroke completed -- waiting for pressure to equalize ... @14:49:00.74 SS.reconfigure :exhale @14:49:00.78 SS.hold @14:49:12.37 Intake.select :close @14:49:12.51 SSV.dial :exhaust @14:49:13.26 Exhaust.select :open @14:49:13.37 SS.seek 0ml @14:49:22.42 Exhaust.select :close @14:49:22.52 SSV.dial CBV @14:49:22.54
CBV.dial SSV @14:49:23.30 Allowing 130s for 25.0ml of remaining 575.0ml @14:49:23.31 Intake.select :open @14:49:25.46 Intake @15.3psia, vacuum from 9.8 to 12.8psi @14:49:25.48 SS.reconfigure :inhaling 0.95ml/s @14:49:25.52 SS.seek 25ml @14:49:52.50 Vacuum too high -- waiting up to 40 seconds @14:49:52.51 SS.reconfigure :exhale @14:49:52.56 SS.hold @14:49:58.20 Intake @13.9psia, vacuum from 8.4 to 11.4psi @14:49:58.21 SS.reconfigure :inhaling 0.87ml/s @14:49:58.26 SS.seek 25ml @14:50:00.38 Stroke completed -- waiting for pressure to equalize ... @14:50:00.39 SS.reconfigure :exhale @14:50:00.44 SS.hold @14:50:06.95 Intake.select :close @14:50:07.08 SSV.dial :exhaust @14:50:07.82 Exhaust.select :open @14:50:07.93 SS.seek 0ml @14:50:16.71 Exhaust.select :close @14:50:16.82 SSV.dial CBV @14:50:16.85
CBV.dial SSV @14:50:17.57 Allowing 130s for 25.0ml of remaining 550.0ml @14:50:17.58 Intake.select :open @14:50:19.74 Intake @15.4psia, vacuum from 9.9 to 12.9psi @14:50:19.75 SS.reconfigure :inhaling 0.87ml/s @14:50:19.80 SS.seek 25ml @14:50:51.10 Vacuum too high -- waiting up to 40 seconds @14:50:51.11 SS.reconfigure :exhale @14:50:51.15 SS.hold @14:50:56.78 Intake @14.1psia, vacuum from 8.6 to 11.6psi @14:50:56.79 SS.reconfigure :inhaling 0.78ml/s @14:50:56.83 SS.seek 25ml @14:50:57.48 Stroke completed -- waiting for pressure to equalize ... @14:50:57.49 SS.reconfigure :exhale @14:50:57.53 SS.hold @14:51:03.09 Intake.select :close @14:51:03.23 SSV.dial :exhaust @14:51:03.96 Exhaust.select :open @14:51:04.07 SS.seek 0ml @14:51:13.09 Exhaust.select :close @14:51:13.19 SSV.dial CBV @14:51:13.21
CBV.dial SSV @14:51:13.96 Allowing 130s for 25.0ml of remaining 525.0ml @14:51:13.97 Intake.select :open @14:51:16.13 Intake @15.3psia, vacuum from 9.8 to 12.8psi @14:51:16.14 SS.reconfigure :inhaling 0.78ml/s @14:51:16.19 SS.seek 25ml @14:51:50.43 Stroke completed -- waiting for pressure to equalize ... @14:51:50.44 SS.reconfigure :exhale @14:51:50.48 SS.hold @14:52:03.12 Intake.select :close @14:52:03.26 SSV.dial :exhaust @14:52:04.01 Exhaust.select :open @14:52:04.12 SS.seek 0ml @14:52:12.96 Exhaust.select :close @14:52:13.07 SSV.dial CBV @14:52:13.11
CBV.dial SSV @14:52:13.84 Allowing 130s for 25.0ml of remaining 500.0ml @14:52:13.85 Intake.select :open @14:52:16.00 Intake @15.4psia, vacuum from 9.9 to 12.9psi @14:52:16.02 SS.reconfigure :inhaling 0.78ml/s @14:52:16.06 SS.seek 25ml @14:52:50.83 Stroke completed -- waiting for pressure to equalize ... @14:52:50.84 SS.reconfigure :exhale @14:52:50.89 SS.hold @14:53:05.24 Intake.select :close @14:53:05.39 SSV.dial :exhaust @14:53:06.13 Exhaust.select :open @14:53:06.24 SS.seek 0ml @14:53:15.02 Exhaust.select :close @14:53:15.13 SSV.dial CBV @14:53:15.18
CBV.dial SSV @14:53:15.88 Allowing 130s for 25.0ml of remaining 475.0ml @14:53:15.89 Intake.select :open @14:53:18.05 Intake @15.3psia, vacuum from 9.8 to 12.8psi @14:53:18.07 SS.reconfigure :inhaling 0.78ml/s @14:53:18.11 SS.seek 25ml @14:53:47.59 Vacuum too high -- waiting up to 40 seconds @14:53:47.60 SS.reconfigure :exhale @14:53:47.64 SS.hold @14:53:55.11 Intake @14.5psia, vacuum from 9.0 to 12.0psi @14:53:55.13 SS.reconfigure :inhaling 0.70ml/s @14:53:55.17 SS.seek 25ml @14:54:01.63 Stroke completed -- waiting for pressure to equalize ... @14:54:01.64 SS.reconfigure :exhale @14:54:01.68 SS.hold @14:54:10.23 Intake.select :close @14:54:10.37 SSV.dial :exhaust @14:54:11.12 Exhaust.select :open @14:54:11.22 SS.seek 0ml @14:54:20.29 Exhaust.select :close @14:54:20.39 SSV.dial CBV @14:54:20.42
CBV.dial SSV @14:54:21.16 Allowing 130s for 25.0ml of remaining 450.0ml @14:54:21.17 Intake.select :open @14:54:23.33 Intake @15.2psia, vacuum from 9.7 to 12.7psi @14:54:23.35 SS.reconfigure :inhaling 0.70ml/s @14:54:23.39 SS.seek 25ml @14:54:58.44 Vacuum too high -- waiting up to 40 seconds @14:54:58.45 SS.reconfigure :exhale @14:54:58.49 SS.hold @14:55:06.43 Intake @14.5psia, vacuum from 9.0 to 12.0psi @14:55:06.44 SS.reconfigure :inhaling 0.63ml/s @14:55:06.49 SS.seek 25ml @14:55:09.85 Stroke completed -- waiting for pressure to equalize ... @14:55:09.86 SS.reconfigure :exhale @14:55:09.90 SS.hold @14:55:18.49 Intake.select :close @14:55:18.63 SSV.dial :exhaust @14:55:19.36 Exhaust.select :open @14:55:19.47 SS.seek 0ml @14:55:28.18 Exhaust.select :close @14:55:28.28 SSV.dial CBV @14:55:28.30
CBV.dial SSV @14:55:29.05 Allowing 130s for 25.0ml of remaining 425.0ml @14:55:29.06 Intake.select :open @14:55:31.22 Intake @15.3psia, vacuum from 9.8 to 12.8psi @14:55:31.23 SS.reconfigure :inhaling 0.63ml/s @14:55:31.28 SS.seek 25ml @14:56:11.81 Vacuum too high -- waiting up to 40 seconds @14:56:11.82 SS.reconfigure :exhale @14:56:11.86 SS.hold @14:56:19.23 Intake @14.5psia, vacuum from 9.0 to 12.0psi @14:56:19.24 SS.reconfigure :inhaling 0.57ml/s @14:56:19.29 SS.seek 25ml @14:56:21.49 Stroke completed -- waiting for pressure to equalize ... @14:56:21.50 SS.reconfigure :exhale @14:56:21.54 SS.hold @14:56:29.00 Intake.select :close @14:56:29.14 SSV.dial :exhaust @14:56:29.88 Exhaust.select :open @14:56:29.99 SS.seek 0ml @14:56:38.81 Exhaust.select :close @14:56:38.92 SSV.dial CBV @14:56:38.94
CBV.dial SSV @14:56:39.69 Allowing 130s for 25.0ml of remaining 400.0ml @14:56:39.70 Intake.select :open @14:56:41.87 Intake @15.2psia, vacuum from 9.7 to 12.7psi @14:56:41.88 SS.reconfigure :inhaling 0.57ml/s @14:56:41.93 SS.seek 25ml @14:57:28.55 Stroke completed -- waiting for pressure to equalize ... @14:57:28.56 SS.reconfigure :exhale @14:57:28.60 SS.hold @14:57:44.87 Intake.select :close @14:57:45.01 SSV.dial :exhaust @14:57:45.75 Exhaust.select :open @14:57:45.86 SS.seek 0ml @14:57:54.61 Exhaust.select :close @14:57:54.71 SSV.dial CBV @14:57:54.74
CBV.dial SSV @14:57:55.49 Allowing 130s for 25.0ml of remaining 375.0ml @14:57:55.50 Intake.select :open @14:57:57.65 Intake @15.3psia, vacuum from 9.8 to 12.8psi @14:57:57.67 SS.reconfigure :inhaling 0.57ml/s @14:57:57.71 SS.seek 25ml @14:58:41.05 Vacuum too high -- waiting up to 40 seconds @14:58:41.06 SS.reconfigure :exhale @14:58:41.11 SS.hold @14:58:50.13 Intake @14.6psia, vacuum from 9.1 to 12.1psi @14:58:50.14 SS.reconfigure :inhaling 0.51ml/s @14:58:50.19 SS.seek 25ml @14:58:54.67 Stroke completed -- waiting for pressure to equalize ... @14:58:54.68 SS.reconfigure :exhale @14:58:54.72 SS.hold @14:59:03.28 Intake.select :close @14:59:03.41 SSV.dial :exhaust @14:59:04.16 Exhaust.select :open @14:59:04.26 SS.seek 0ml @14:59:13.09 Exhaust.select :close @14:59:13.20 SSV.dial CBV @14:59:13.22
CBV.dial SSV @14:59:13.97 Allowing 130s for 25.0ml of remaining 350.0ml @14:59:13.98 Intake.select :open @14:59:16.15 Intake @15.4psia, vacuum from 9.9 to 12.9psi @14:59:16.16 SS.reconfigure :inhaling 0.51ml/s @14:59:16.21 SS.seek 25ml @15:00:09.11 Stroke completed -- waiting for pressure to equalize ... @15:00:09.12 SS.reconfigure :exhale @15:00:09.16 SS.hold @15:00:25.50 Intake.select :close @15:00:25.64 SSV.dial :exhaust @15:00:26.37 Exhaust.select :open @15:00:26.48 SS.seek 0ml @15:00:35.28 Exhaust.select :close @15:00:35.38 SSV.dial CBV @15:00:35.40
CBV.dial SSV @15:00:36.16 Allowing 130s for 25.0ml of remaining 325.0ml @15:00:36.17 Intake.select :open @15:00:38.32 Intake @15.2psia, vacuum from 9.7 to 12.7psi @15:00:38.34 SS.reconfigure :inhaling 0.51ml/s @15:00:38.38 SS.seek 25ml @15:01:28.61 Vacuum too high -- waiting up to 40 seconds @15:01:28.62 SS.reconfigure :exhale @15:01:28.67 SS.hold @15:01:38.32 Intake @14.7psia, vacuum from 9.2 to 12.2psi @15:01:38.33 SS.reconfigure :inhaling 0.47ml/s @15:01:38.38 SS.seek 25ml @15:01:42.35 Stroke completed -- waiting for pressure to equalize ... @15:01:42.36 SS.reconfigure :exhale @15:01:42.41 SS.hold @15:01:52.63 Intake.select :close @15:01:52.77 SSV.dial :exhaust @15:01:53.52 Exhaust.select :open @15:01:53.62 SS.seek 0ml @15:02:02.62 Exhaust.select :close @15:02:02.73 SSV.dial CBV @15:02:02.76
CBV.dial SSV @15:02:03.50 Allowing 130s for 25.0ml of remaining 300.0ml @15:02:03.51 Intake.select :open @15:02:05.67 Intake @15.2psia, vacuum from 9.7 to 12.7psi @15:02:05.69 SS.reconfigure :inhaling 0.47ml/s @15:02:05.73 SS.seek 25ml @15:03:05.04 Stroke completed -- waiting for pressure to equalize ... @15:03:05.05 SS.reconfigure :exhale @15:03:05.09 SS.hold @15:03:25.52 Intake.select :close @15:03:25.66 SSV.dial :exhaust @15:03:26.41 Exhaust.select :open @15:03:26.51 SS.seek 0ml @15:03:35.67 Exhaust.select :close @15:03:35.77 SSV.dial CBV @15:03:35.79
CBV.dial SSV @15:03:36.54 Allowing 130s for 25.0ml of remaining 275.0ml @15:03:36.55 Intake.select :open @15:03:38.71 Intake @15.1psia, vacuum from 9.6 to 12.6psi @15:03:38.73 SS.reconfigure :inhaling 0.47ml/s @15:03:38.77 SS.seek 25ml @15:04:36.02 Vacuum too high -- waiting up to 40 seconds @15:04:36.03 SS.reconfigure :exhale @15:04:36.07 SS.hold @15:04:47.13 Intake @14.6psia, vacuum from 9.1 to 12.1psi @15:04:47.14 SS.reconfigure :inhaling 0.43ml/s @15:04:47.19 SS.seek 25ml @15:04:49.99 Stroke completed -- waiting for pressure to equalize ... @15:04:50.00 SS.reconfigure :exhale @15:04:50.04 SS.hold @15:05:02.75 Intake.select :close @15:05:02.89 SSV.dial :exhaust @15:05:03.63 Exhaust.select :open @15:05:03.74 SS.seek 0ml @15:05:12.55 Exhaust.select :close @15:05:12.66 SSV.dial CBV @15:05:12.68
CBV.dial SSV @15:05:13.43 Allowing 130s for 25.0ml of remaining 250.0ml @15:05:13.44 Intake.select :open @15:05:15.60 Intake @15.1psia, vacuum from 9.6 to 12.6psi @15:05:15.61 SS.reconfigure :inhaling 0.43ml/s @15:05:15.65 SS.seek 25ml @15:06:26.94 Stroke completed -- waiting for pressure to equalize ... @15:06:26.95 SS.reconfigure :exhale @15:06:26.99 SS.hold @15:06:47.35 Intake.select :close @15:06:47.49 SSV.dial :exhaust @15:06:48.23 Exhaust.select :open @15:06:48.34 SS.seek 0ml @15:06:57.19 Exhaust.select :close @15:06:57.30 SSV.dial CBV @15:06:57.33
CBV.dial SSV @15:06:58.07 Allowing 130s for 25.0ml of remaining 225.0ml @15:06:58.08 Intake.select :open @15:07:00.23 Intake @15.2psia, vacuum from 9.7 to 12.7psi @15:07:00.25 SS.reconfigure :inhaling 0.43ml/s @15:07:00.29 SS.seek 25ml @15:08:08.29 Vacuum too high -- waiting up to 40 seconds @15:08:08.30 SS.reconfigure :exhale @15:08:08.34 SS.hold @15:08:21.50 Intake @14.9psia, vacuum from 9.4 to 12.4psi @15:08:21.51 SS.reconfigure :inhaling 0.38ml/s @15:08:21.55 SS.seek 25ml @15:08:25.21 Stroke completed -- waiting for pressure to equalize ... @15:08:25.22 SS.reconfigure :exhale @15:08:25.26 SS.hold @15:08:37.93 Intake.select :close @15:08:38.07 SSV.dial :exhaust @15:08:38.82 Exhaust.select :open @15:08:38.92 SS.seek 0ml @15:08:47.74 Exhaust.select :close @15:08:47.84 SSV.dial CBV @15:08:47.86
CBV.dial SSV @15:08:48.61 Allowing 130s for 25.0ml of remaining 200.0ml @15:08:48.62 Intake.select :open @15:08:50.78 Intake @15.3psia, vacuum from 9.8 to 12.8psi @15:08:50.79 SS.reconfigure :inhaling 0.38ml/s @15:08:50.84 SS.seek 25ml @15:10:17.70 Stroke completed -- waiting for pressure to equalize ... @15:10:17.71 SS.reconfigure :exhale @15:10:17.75 SS.hold @15:10:42.20 Intake.select :close @15:10:42.33 SSV.dial :exhaust @15:10:43.06 Exhaust.select :open @15:10:43.17 SS.seek 0ml @15:10:52.02 Exhaust.select :close @15:10:52.12 SSV.dial CBV @15:10:52.14
CBV.dial SSV @15:10:53.38 Allowing 130s for 25.0ml of remaining 175.0ml @15:10:53.39 Intake.select :open @15:10:55.54 Intake @15.2psia, vacuum from 9.7 to 12.7psi @15:10:55.56 SS.reconfigure :inhaling 0.38ml/s @15:10:55.60 SS.seek 25ml @15:12:23.45 Stroke completed -- waiting for pressure to equalize ... @15:12:23.46 SS.reconfigure :exhale @15:12:23.54 Ignoring excess vacuum after stroke's end ... @15:12:23.55 SS.reconfigure :exhale @15:12:23.59 SS.hold @15:12:50.04 Intake.select :close @15:12:50.18 SSV.dial :exhaust @15:12:50.93 Exhaust.select :open @15:12:51.03 SS.seek 0ml @15:12:59.87 Exhaust.select :close @15:12:59.98 SSV.dial CBV @15:13:00.01
CBV.dial SSV @15:13:00.73 Allowing 130s for 25.0ml of remaining 150.0ml @15:13:00.74 Intake.select :open @15:13:02.90 Intake @15.2psia, vacuum from 9.7 to 12.7psi @15:13:02.92 SS.reconfigure :inhaling 0.38ml/s @15:13:02.96 SS.seek 25ml @15:14:15.04 Vacuum too high -- waiting up to 40 seconds @15:14:15.05 SS.reconfigure :exhale @15:14:15.09 SS.hold @15:14:29.34 Intake @14.8psia, vacuum from 9.3 to 12.3psi @15:14:29.36 SS.reconfigure :inhaling 0.34ml/s @15:14:29.40 SS.seek 25ml @15:14:44.46 Stroke completed -- waiting for pressure to equalize ... @15:14:44.47 SS.reconfigure :exhale @15:14:44.51 SS.hold @15:15:06.92 Intake.select :close @15:15:07.05 SSV.dial :exhaust @15:15:07.79 Exhaust.select :open @15:15:07.90 SS.seek 0ml @15:15:16.73 Exhaust.select :close @15:15:16.83 SSV.dial CBV @15:15:16.85
CBV.dial SSV @15:15:17.61 Allowing 130s for 25.0ml of remaining 125.0ml @15:15:17.62 Intake.select :open @15:15:19.77 Intake @15.2psia, vacuum from 9.7 to 12.7psi @15:15:19.79 SS.reconfigure :inhaling 0.34ml/s @15:15:19.83 SS.seek 25ml @15:17:01.21 Stroke completed -- waiting for pressure to equalize ... @15:17:01.22 SS.reconfigure :exhale @15:17:01.26 SS.hold @15:17:27.63 SS.reconfigure :default @15:17:27.67 SS.hold @15:17:27.77 Intake.select :close @15:17:27.92 ShallowSampler::Clogged in MAIN -- Filtering too Slowly after 900.0ml @15:17:27.93 SSV.dial :exhaust @15:17:28.66 Exhaust.select :open @15:17:28.77 SS.reconfigure :exhale @15:17:28.81 SS.seek 0ml @15:17:37.59 Exhaust.select :close @15:17:37.69 Sampled 900.0ml @15:17:37.70 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @15:17:37.71 Intake.select :close @15:17:37.77 CSV.dial :waste @15:17:37.84 CS.seek 0ml @15:17:37.93 SSV.dial CBV,avoiding: :exhaust @15:17:37.95 CSV.dial :air @15:17:37.97
CBV.dial SSV @15:17:38.75 CS.seek 10ml @15:17:39.82
CTV.dial :air @15:17:40.61 delay 15 seconds @15:17:55.63 SSV.dial :spit,avoiding: :exhaust @15:17:56.94 CTV.dial CSV,avoiding: :intake @15:17:56.99
SS.seek 0ml @15:17:57.08 SSV.dial CBV,avoiding: :exhaust @15:18:03.25 CBV.dial between SSV and waste2 @15:18:03.63 CTV.dial CSV,avoiding: :air @15:18:03.65
CSV.dial CTV @15:18:04.23 CS.reconfigure :pressurize @15:18:04.28 CS.seek 0ml @15:18:22.32 BubblePoint: Intake @39.2psia, Outlet @14.7psia @15:18:22.33 CBV.dial SSV @15:18:22.67 SS.reconfigure :default @15:18:22.71 SS.seek 10ml @15:18:28.34 delay 15 seconds @15:18:43.45 Evacuated: Intake @16.6psia, Outlet @9.0psia @15:18:43.46 SSV.dial :spit,avoiding: :exhaust @15:18:44.76 SS.reconfigure :exhale @15:18:44.81 SS.seek 0ml @15:18:50.19 SS.reconfigure :fast @15:18:50.23 SS.seek 5ml @15:18:53.16 SSV.dial CBV,avoiding: :exhaust @15:18:53.17
CBV.dial SSV @15:18:54.48 CTV.dial :air,avoiding: :intake @15:18:55.82 CSV.dial CTV @15:18:55.85
SS.seek 10ml @15:18:56.84 CSV.dial :air @15:18:57.29 CS.reconfigure :fast @15:18:57.33 CS.seek 0ml @15:18:58.32
SSV.dial :spit,avoiding: :exhaust @15:18:59.64 SS.reconfigure :exhale @15:18:59.68 SS.seek 0ml @15:19:04.94 SS.reconfigure :fast @15:19:04.98 SS.seek 5ml @15:19:05.31 CS.stop @15:19:08.03 SSV.dial CBV,avoiding: :exhaust @15:19:08.05
CBV.dial SSV @15:19:10.38 SSV.dial :spit,avoiding: :exhaust @15:19:11.70 SS.reconfigure :default @15:19:11.74 SS.seek 0ml @15:19:14.88 SS.stop @15:19:14.92 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @15:19:14.93 Intake.select :close @15:19:15.00 CSV.dial :waste @15:19:15.12 SSV.dial CBV,avoiding: :exhaust @15:19:15.13
CBV.dial SSV @15:19:15.74 CS.seek 0ml @15:19:15.81 CSV.dial :air @15:19:16.45
CTV.dial :air @15:19:16.46 delay 15 seconds @15:19:16.54 CS.seek 10ml @15:19:31.48
SSV.dial :spit,avoiding: :exhaust @15:19:32.78 CTV.dial CSV,avoiding: :intake @15:19:32.81
SS.reconfigure :exhale @15:19:32.90 SS.seek 0ml @15:19:33.27 SSV.dial CBV,avoiding: :exhaust @15:19:40.96 CBV.dial between SSV and waste2 @15:19:41.37 CTV.dial CSV,avoiding: :air @15:19:41.38
CSV.dial CTV @15:19:42.27 CS.reconfigure :pressurize @15:19:42.32 CS.seek 0ml @15:20:03.38 BubblePoint: Intake @37.8psia, Outlet @13.7psia @15:20:03.39 CBV.dial SSV @15:20:03.71 SS.reconfigure :default @15:20:03.75 SS.seek 10ml @15:20:09.36 delay 15 seconds @15:20:24.48 Evacuated: Intake @18.3psia, Outlet @11.1psia @15:20:24.49 SSV.dial :spit,avoiding: :exhaust @15:20:25.81 SS.reconfigure :exhale @15:20:25.85 SS.seek 0ml @15:20:31.28 SS.reconfigure :fast @15:20:31.32 SS.seek 5ml @15:20:34.23 SSV.dial CBV,avoiding: :exhaust @15:20:34.25
CBV.dial SSV @15:20:35.57 CTV.dial :air,avoiding: :intake @15:20:36.91 CSV.dial CTV @15:20:36.95
SS.seek 10ml @15:20:37.94 CSV.dial :air @15:20:38.40 CS.reconfigure :fast @15:20:38.44 CS.seek 0ml @15:20:39.43
SSV.dial :spit,avoiding: :exhaust @15:20:40.75 SS.reconfigure :exhale @15:20:40.79 SS.seek 0ml @15:20:43.72 CS.stop @15:20:46.08
SS.reconfigure :fast @15:20:46.12 SS.seek 5ml @15:20:49.00 SSV.dial CBV,avoiding: :exhaust @15:20:49.02
CBV.dial SSV @15:20:51.33 SSV.dial :spit,avoiding: :exhaust @15:20:52.62 SS.reconfigure :default @15:20:52.66 SS.seek 0ml @15:20:56.00 SS.stop @15:20:56.04 Lysing: Adding 2.0ml AqMeOH @15:20:56.05 CBV.dial between CSV and waste1,avoiding: :DBV @15:20:57.53 CTV.dial CSV @15:20:58.84 CSV.dial CSR @15:20:59.63 Pulling 1ml of CSR.AqMeOH @15:20:59.64 CSR.select :AqMeOH @15:20:59.75 CS.reconfigure :default @15:20:59.79 CS.seek 1ml @15:21:03.38 delay 5 seconds @15:21:08.40 CSR.select :air @15:21:08.55 CS.seek 6ml @15:21:22.28 delay 2 seconds @15:21:24.30 CSV.dial :waste2 @15:21:24.74 CS.reconfigure :fast @15:21:24.78 CS.seek 0ml @15:21:39.12 CSV.dial CSR @15:21:39.63 Pulling 2ml of CSR.AqMeOH @15:21:39.64 CSR.select :AqMeOH @15:21:39.75 CS.reconfigure :default @15:21:39.79 CS.seek 2ml @15:21:45.69 delay 5 seconds @15:21:50.71 CSR.select :air @15:21:50.86 CS.seek 5ml @15:21:59.53 delay 2 seconds @15:22:01.55 CSV.dial CTV @15:22:01.99 CS.seek 1ml @15:22:13.32 Before lysing Collection Puck Heater at 21C/39.1psia @15:22:13.34 Heating Puck.from 2, :sh1 in Collection Clamp to 70C for 10:00 @15:22:13.36 CH.seek 70C,8:00 @15:24:04.03 CH.hold 10:00,70C @15:24:04.20 Begun lysing Collection Puck Heater at 68C/46.5psia @15:34:04.22 Ended lysing Collection Puck Heater at 70C/45.0psia @15:34:04.23 CS.seek 0ml @15:34:07.87 CTV.dial between spare and air,avoiding: :intake @15:34:08.91 CSV.dial :air @15:34:09.36 CS.seek 5ml @15:34:23.14 CSV.dial CTV @15:34:23.60 delay 2 seconds @15:34:25.61 CSV.dial :air @15:34:26.06 delay 2 seconds @15:34:28.08 CSV.dial CTV @15:34:28.54 delay 2 seconds @15:34:30.55 CSV.dial :air @15:34:31.00 delay 2 seconds @15:34:33.02 CS.seek 0ml @15:34:46.60 Puck @74.1psia after final lysis pressurization @15:34:46.61 Recovering Lysate into Collection syringe @15:34:46.62 CSV.dial CBV @15:34:47.61 CBV.dial CSV @15:34:47.98 CS.seek 5ml @15:35:01.55 delay 5 seconds @15:35:06.57 CTV.dial :air @15:35:07.09 CS.seek 7ml @15:35:12.98 delay 5 seconds @15:35:18.00 CSV.dial :air2 @15:35:18.44 CS.seek 10ml @15:35:27.02 CSV.dial between air and CTV,avoiding: CBV @15:35:28.29 Lysing Complete @15:35:28.35 Storing sh1 puck in Collection Clamp to tube 1 @15:35:28.51 SC.reconfigure :tight @15:35:28.56 SC.seek 1 @15:35:32.14 SC.configure :limp @15:35:32.34 Elbow.reconfigure :tight @15:35:32.38 Elbow.seek 1 @15:35:34.13 Forearm.reconfigure :tight @15:35:34.17 Forearm.seek 1 + 450 counts @15:35:35.14 SE.reconfigure :pick @15:35:35.19 SE.seek :beamBreak @15:35:37.50 SE.configure :default @15:35:37.64 Elbow.reconfigure :limp @15:35:37.68 Forearm.reconfigure :loose @15:35:37.72 Forearm.seek 1 @15:35:38.00 Elbow.hold @15:35:38.06 Elbow.reconfigure :tight @15:35:38.15 SE.seek 11.09 Pucks below Top Plate @15:35:38.55 Forearm.force -20 @15:35:38.59 Forearm.seek 1 + 274 counts @15:35:38.88 Forearm.reconfigure :tight @15:35:38.92 Forearm.seek 1 + 450 counts @15:35:39.13 SE.seek 12.09 Pucks below Top Plate @15:35:39.73 Forearm.reconfigure :loose @15:35:39.78 Forearm.seek :retract @15:35:40.71 Elbow.seek CC @15:35:42.32 Elbow.reconfigure :loose @15:35:42.41 Forearm.seek CC @15:35:43.36 Hand.close @15:35:43.99 CC.seek :open @15:35:48.70 Forearm.seek :retract @15:35:49.67 Elbow.reconfigure :tight @15:35:49.72 Elbow.seek 1 @15:35:51.47 Elbow.reconfigure :limp @15:35:51.51 Forearm.seek 1 @15:35:52.48 Elbow.hold @15:35:52.54 Elbow.reconfigure :tight @15:35:52.59 Hand.open @15:35:53.35 Forearm.force -20 @15:35:53.38 Forearm.seek 1 + 310 counts @15:35:54.17 Forearm.reconfigure :tight @15:35:54.21 Forearm.seek 1 + 450 counts @15:35:54.47 SE.seek :down @15:35:55.60
Forearm.reconfigure :loose @15:35:55.64 Forearm.seek :retract @15:35:56.35 SE.stop @15:35:56.57
Tranferring DA @15:35:56.58 Beginning samplePassC @15:35:56.59 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @15:35:56.77 Elbow.seek FlushPuck::Garage @15:35:58.60 Elbow.reconfigure :loose @15:35:58.69 Forearm.seek :garage @15:35:59.69 Hand.close @15:36:00.35 Forearm.seek :retract @15:36:01.30 Elbow.reconfigure :tight @15:36:01.34 Elbow.seek CC @15:36:04.23 Elbow.reconfigure :loose @15:36:04.31 Forearm.reconfigure :tight @15:36:04.36 Forearm.seek CC @15:36:05.57 CC.seek :closed @15:36:10.09 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.35 @15:36:10.17 Hand.open @15:36:10.79 Forearm.reconfigure :loose @15:36:10.84 Forearm.seek :retract @15:36:11.86 PSV.dial PSR @15:36:13.23 Pulling 1.5ml of PSR.flush @15:36:13.24 PSR.select :flush @15:36:13.36 PS.seek 1.5ml @15:36:29.55 delay 5 seconds @15:36:34.57 PSV.dial :waste1 @15:36:35.03 PS.seek 0ml @15:36:51.14 PSV.dial PSR @15:36:51.60 PSR.select :air @15:36:51.71 PS.seek 2.5ml @15:37:18.09 PRV.dial CBV,avoiding: :ab2 @15:37:18.11
CBV.dial PRV @15:37:19.78 PSV.dial :mixloop @15:37:20.82 CTV.dial :air,avoiding: :intake @15:37:20.83 PTV.dial :PRVmixing @15:37:22.14 PS.seek 0ml @15:37:48.55 PSV.dial :air @15:37:49.01 PS.seek 2.5ml @15:38:15.36 PSV.dial :mixloop @15:38:15.82 PS.seek 0ml @15:38:42.20 PSV.dial :air @15:38:42.68 PS.seek 2.5ml @15:39:09.08 PSV.dial :mixloop @15:39:09.54 PS.seek 0ml @15:39:35.86 Garaging FlushPuck in Collection Clamp @15:39:35.93 Elbow.reconfigure :tight @15:39:35.98 Elbow.seek CC @15:39:36.32 Elbow.reconfigure :loose @15:39:36.41 Forearm.seek CC @15:39:37.43 Hand.close @15:39:37.98 CC.seek :open @15:39:42.59 Forearm.seek :retract @15:39:43.58 Elbow.reconfigure :tight @15:39:43.62 Elbow.seek :garage @15:39:46.55 Elbow.reconfigure :loose @15:39:46.64 Forearm.reconfigure :tight @15:39:46.68 Forearm.seek :garage @15:39:47.70 Hand.open @15:39:48.41 Forearm.reconfigure :loose @15:39:48.45 Forearm.seek :retract @15:39:49.43 PSV.dial :mixloop @15:39:49.45 PTV.dial :PRVmixing @15:39:49.46 PRV.dial CBV @15:39:49.95
CBV.dial PRV @15:39:49.97 CTV.dial :air,avoiding: :intake @15:39:49.99 Loading lyfil puck from tube 2 into Collection Clamp @15:39:50.22 SC.reconfigure :tight @15:39:50.26 SC.seek 2 @15:39:53.93 SC.configure :limp @15:39:54.13 Elbow.reconfigure :tight @15:39:54.17 Elbow.seek 2 @15:39:56.08 Forearm.reconfigure :tight @15:39:56.13 Forearm.seek 2 + 450 counts @15:39:57.26 SE.reconfigure :pick @15:39:57.31 SE.seek :beamBreak @15:39:59.26 SE.configure :default @15:39:59.41 Elbow.seek 2 @15:39:59.58 Forearm.seek 2 + 300 counts @15:39:59.75 Forearm.reconfigure :loose @15:39:59.79 Hand.close @15:40:00.37 SE.seek :down @15:40:00.56
Forearm.seek :retract @15:40:01.51 Elbow.seek CC @15:40:01.88 SE.stop @15:40:03.16
Elbow.reconfigure :loose @15:40:03.26 Forearm.reconfigure :tight @15:40:03.30 Forearm.seek CC @15:40:04.57 CC.seek :closed @15:40:09.09 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.05 @15:40:09.10 Hand.open @15:40:09.79 Forearm.reconfigure :loose @15:40:09.83 Forearm.seek :retract @15:40:10.81 CTV.dial CSV,avoiding: :intake @15:40:12.17 CSV.dial CTV @15:40:12.46 PS.seek 2.5ml @15:40:38.79 CS.seek 4.5ml @15:40:53.64 delay 8 seconds @15:41:01.66 PSV.dial :air @15:41:02.12 delay 2 seconds @15:41:04.14 PSV.dial :waste2 @15:41:04.88 PS.seek 0ml @15:41:31.23 CBV.dial between PRV and waste1 @15:41:31.56 CSV.dial :air @15:41:32.02 CS.seek 10ml @15:41:47.10 CSV.dial CTV @15:41:47.55 CS.seek 0ml @15:42:13.91 CBV.dial :waste1 @15:42:14.22 delay 5 seconds @15:42:19.24 CTV.dial :air,avoiding: :intake @15:42:20.64 Storing lyfil puck in Collection Clamp to tube 1 @15:42:20.80 SC.reconfigure :tight @15:42:20.85 SC.seek 1 @15:42:24.43 SC.configure :limp @15:42:24.62 Elbow.reconfigure :tight @15:42:24.66 Elbow.seek 1 @15:42:26.42 Forearm.reconfigure :tight @15:42:26.46 Forearm.seek 1 + 450 counts @15:42:27.71 SE.reconfigure :pick @15:42:27.76 SE.seek :beamBreak @15:42:30.06 SE.configure :default @15:42:30.20 Elbow.reconfigure :limp @15:42:30.25 Forearm.reconfigure :loose @15:42:30.29 Forearm.seek 1 @15:42:30.54 Elbow.hold @15:42:30.60 Elbow.reconfigure :tight @15:42:31.16 SE.seek 12.1 Pucks below Top Plate @15:42:31.71 Forearm.force -20 @15:42:31.74 Forearm.seek 1 + 305 counts @15:42:32.05 Forearm.reconfigure :tight @15:42:32.09 Forearm.seek 1 + 450 counts @15:42:32.30 SE.seek 13.1 Pucks below Top Plate @15:42:32.92 Forearm.reconfigure :loose @15:42:32.96 Forearm.seek :retract @15:42:33.92 Elbow.seek CC @15:42:35.53 Elbow.reconfigure :loose @15:42:35.62 Forearm.seek CC @15:42:36.59 Hand.close @15:42:37.23 CC.seek :open @15:42:41.94 Forearm.seek :retract @15:42:42.89 Elbow.reconfigure :tight @15:42:42.93 Elbow.seek 1 @15:42:44.72 Elbow.reconfigure :limp @15:42:44.76 Forearm.seek 1 @15:42:45.74 Elbow.hold @15:42:45.80 Elbow.reconfigure :tight @15:42:45.85 Hand.open @15:42:46.59 Forearm.force -20 @15:42:46.62 Forearm.seek 1 + 312 counts @15:42:46.93 Forearm.reconfigure :tight @15:42:46.97 Forearm.seek 1 + 450 counts @15:42:47.24 SE.seek :down @15:42:48.37
Forearm.reconfigure :loose @15:42:48.41 Forearm.seek :retract @15:42:49.05 SE.stop @15:42:49.35
PSV.dial :air @15:42:50.10 PS.seek 2.5ml @15:43:16.45 delay 1 second @15:43:17.46 PSV.dial :mixloop @15:43:17.90 delay 1 second @15:43:18.92 PSV.dial :air @15:43:19.37 delay 1 second @15:43:20.39 PSV.dial :mixloop @15:43:20.84 delay 1 second @15:43:21.85 PSV.dial :air @15:43:22.36 delay 1 second @15:43:23.38 PS.seek 0ml @15:43:49.73 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @15:43:49.90 Elbow.seek FlushPuck::Garage @15:43:51.67 Elbow.reconfigure :loose @15:43:51.76 Forearm.seek :garage @15:43:52.74 Hand.close @15:43:53.46 Forearm.seek :retract @15:43:54.43 Elbow.reconfigure :tight @15:43:54.47 Elbow.seek PC @15:43:55.72 Elbow.reconfigure :loose @15:43:55.81 Forearm.reconfigure :tight @15:43:55.85 Forearm.seek PC @15:43:57.07 PC.seek :closed @15:44:01.71 Processing Clamp onSpring/offSpring for 16/26 samples, on/off torque 1.57 @15:44:01.78 Hand.open @15:44:02.41 Forearm.reconfigure :loose @15:44:02.45 Forearm.seek :retract @15:44:03.41 PRV.dial :air @15:44:03.85 PSV.dial :mixloop @15:44:04.38 PS.seek 0.3ml @15:44:08.81 PSV.dial PSR @15:44:09.92 Pulling 0.4ml of PSR.air @15:44:09.93 PSR.select :air @15:44:09.96 PS.seek 0.7ml @15:44:14.82 delay 3 seconds @15:44:17.84 PSR.select :air @15:44:17.86 PS.seek 2.5ml @15:44:37.04 delay 3 seconds @15:44:40.06 PSV.dial :mixloop @15:44:41.10 PTV.dial :mixing @15:44:41.85 PBV.dial :waste2 @15:44:42.80 PS.seek 0ml @15:45:09.14 Garaging FlushPuck in Processing Clamp @15:45:09.21 Elbow.reconfigure :tight @15:45:09.25 Elbow.seek PC @15:45:09.36 Elbow.reconfigure :loose @15:45:09.45 Forearm.seek PC @15:45:10.43 Hand.close @15:45:10.98 PC.seek :open @15:45:15.68 Forearm.seek :retract @15:45:16.68 Elbow.reconfigure :tight @15:45:16.73 Elbow.seek :garage @15:45:18.08 Elbow.reconfigure :loose @15:45:18.17 Forearm.reconfigure :tight @15:45:18.21 Forearm.seek :garage @15:45:19.15 Hand.open @15:45:19.79 Forearm.reconfigure :loose @15:45:19.83 Forearm.seek :retract @15:45:20.84 Loading da puck from tube 2 into Processing Clamp @15:45:21.07 SC.reconfigure :tight @15:45:21.11 SC.seek 2 @15:45:25.37 SC.configure :limp @15:45:25.57 Elbow.reconfigure :tight @15:45:25.61 Elbow.seek 2 @15:45:27.45 Forearm.reconfigure :tight @15:45:27.49 Forearm.seek 2 + 450 counts @15:45:28.52 SE.reconfigure :pick @15:45:28.57 SE.seek :beamBreak @15:45:30.41 SE.configure :default @15:45:30.55 Elbow.seek 2 @15:45:30.72 Forearm.seek 2 + 300 counts @15:45:30.90 Forearm.reconfigure :loose @15:45:30.94 Hand.close @15:45:31.61 SE.seek :down @15:45:31.81
Forearm.seek :retract @15:45:32.76 Elbow.seek PC @15:45:33.19 SE.stop @15:45:35.47
Elbow.reconfigure :loose @15:45:35.56 Forearm.reconfigure :tight @15:45:35.60 Forearm.seek PC @15:45:36.76 PC.seek :closed @15:45:41.37 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.57 @15:45:41.38 Hand.open @15:45:42.03 Forearm.reconfigure :loose @15:45:42.08 Forearm.seek :retract @15:45:43.02 Heating Puck.from 2, :da in Processing Clamp to 28C for 2:00:00 @15:45:43.04 PH.seek 28C,5:00 @15:46:12.44 PH.hold 2:00:00,28C @15:46:12.50 delay 1:30 @15:47:42.53 PSR.select :air @15:47:42.54 PSV.dial PSR @15:47:43.64 Pulling 0.5ml of PSR.wash @15:47:43.65 PSR.select :wash @15:47:44.24 PS.seek 0.5ml @15:47:50.23 delay 5 seconds @15:47:55.24 PSR.select :air @15:47:55.36 PS.seek 2ml @15:48:11.47 PTV.dial :puck @15:48:12.21 PSV.dial PTV @15:48:13.08 PBV.dial :waste1 @15:48:14.48 PS.seek 1.5ml @15:48:20.41 delay 3 seconds @15:48:23.43 PBV.dial between PSV and waste1 @15:48:23.78 PS.seek 0ml @15:48:39.91 delay 5:00 @15:53:39.93 recoverPSRreagent08 @15:53:39.94 PTV.dial :puck @15:53:39.95 PBV.dial :waste1 @15:53:40.31 delay 30 seconds @15:54:10.34 PBV.dial between PSV and waste1 @15:54:10.69 PSR.select :air @15:54:10.70 PSV.dial PSR @15:54:11.44 PS.reconfigure :fast @15:54:11.49 PS.seek 2.5ml @15:54:35.06 PSV.dial PTV @15:54:35.94 delay 5 seconds @15:54:40.95 PSV.dial PSR @15:54:41.69 delay 5 seconds @15:54:46.70 PSV.dial PTV @15:54:47.45 delay 5 seconds @15:54:52.47 PSV.dial :air @15:54:53.22 PS.seek 0ml @15:55:16.19 PTV.dial :PRVmixing,via: PRV @15:55:17.48 PSV.dial :mixloop @15:55:17.95 PRV.dial :ab1 @15:55:18.71 PS.reconfigure :slow @15:55:18.75 PS.seek 0.15ml @15:55:22.53 delay 2 seconds @15:55:24.55 CBV.dial PRV @15:55:24.58
PRV.dial CBV @15:55:25.11 PS.seek 0.35ml @15:55:29.65 delay 2 seconds @15:55:31.67 CBV.dial between PRV and PSV @15:55:31.98 PRV.dial :air @15:55:32.44 PS.seek 0.9ml @15:55:44.12 PRV.dial :waste2 @15:55:44.85 PS.seek 0.5ml @15:55:53.46 addPMLReagent08 @15:55:53.47 PSV.dial :air @15:55:53.94 PS.reconfigure :default @15:55:53.98 PS.seek 2ml @15:56:10.18 PSV.dial :PML @15:56:10.64 PTV.dial :mixing @15:56:11.39 PBV.dial :waste1 @15:56:11.79 PS.seek 1.85ml @15:56:14.09 PBV.dial between PSV and waste1 @15:56:14.47 PS.seek 0ml @15:56:34.18 delay 2 seconds @15:56:36.20 Beginning samplePassC @15:56:36.21 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @15:56:36.38 Elbow.reconfigure :tight @15:56:36.42 Elbow.seek FlushPuck::Garage @15:56:37.76 Elbow.reconfigure :loose @15:56:37.84 Forearm.seek :garage @15:56:38.86 Hand.close @15:56:39.58 Forearm.seek :retract @15:56:40.55 Elbow.reconfigure :tight @15:56:40.59 Elbow.seek CC @15:56:43.41 Elbow.reconfigure :loose @15:56:43.50 Forearm.reconfigure :tight @15:56:43.54 Forearm.seek CC @15:56:44.74 CC.seek :closed @15:56:49.27 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.25 @15:56:49.33 Hand.open @15:56:50.03 Forearm.reconfigure :loose @15:56:50.07 Forearm.seek :retract @15:56:51.03 PSV.dial PSR @15:56:52.13 Pulling 1.5ml of PSR.flush @15:56:52.14 PSR.select :flush @15:56:52.72 PS.seek 1.5ml @15:57:08.87 delay 5 seconds @15:57:13.89 PSV.dial :waste1 @15:57:14.35 PS.seek 0ml @15:57:30.49 PSV.dial PSR @15:57:30.96 PSR.select :air @15:57:31.08 PS.seek 2.5ml @15:57:57.50 PRV.dial CBV,avoiding: :ab2 @15:57:57.52
CBV.dial PRV @15:57:59.12 PSV.dial :mixloop @15:58:00.15 CTV.dial :air,avoiding: :intake @15:58:00.16 PTV.dial :PRVmixing @15:58:00.91 PS.seek 0ml @15:58:27.30 PSV.dial :air @15:58:27.88 PS.seek 2.5ml @15:58:54.27 PSV.dial :mixloop @15:58:54.73 PS.seek 0ml @15:59:21.05 PSV.dial :air @15:59:21.51 PS.seek 2.5ml @15:59:47.93 PSV.dial :mixloop @15:59:48.39 PS.seek 0ml @16:00:14.80 Garaging FlushPuck in Collection Clamp @16:00:14.87 Elbow.reconfigure :tight @16:00:14.92 Elbow.seek CC @16:00:15.25 Elbow.reconfigure :loose @16:00:15.34 Forearm.seek CC @16:00:16.37 Hand.close @16:00:16.97 CC.seek :open @16:00:21.57 Forearm.seek :retract @16:00:22.54 Elbow.reconfigure :tight @16:00:22.59 Elbow.seek :garage @16:00:25.47 Elbow.reconfigure :loose @16:00:25.56 Forearm.reconfigure :tight @16:00:25.60 Forearm.seek :garage @16:00:26.62 Hand.open @16:00:27.27 Forearm.reconfigure :loose @16:00:27.32 Forearm.seek :retract @16:00:28.29 delayUntil "16:04:36.19" @16:00:28.32 Whole Cell Archive sampling 1000ml @16:00:28.34 Loading wcr puck from tube 2 into Collection Clamp @16:00:28.56 SC.reconfigure :tight @16:00:28.61 SC.seek 2 @16:00:28.96 SC.configure :limp @16:00:29.16 Elbow.reconfigure :tight @16:00:29.20 Elbow.seek 2 @16:00:31.17 Forearm.reconfigure :tight @16:00:31.22 Forearm.seek 2 + 450 counts @16:00:32.23 SE.reconfigure :pick @16:00:32.29 SE.seek :beamBreak @16:00:34.27 SE.configure :default @16:00:34.42 Elbow.seek 2 @16:00:34.91 Forearm.seek 2 + 300 counts @16:00:35.17 Forearm.reconfigure :loose @16:00:35.21 Hand.close @16:00:35.85 SE.seek :down @16:00:36.05 Forearm.seek :retract @16:00:37.06 Elbow.seek CC @16:00:37.47 SE.stop @16:00:38.76 Elbow.reconfigure :loose @16:00:38.85 Forearm.reconfigure :tight @16:00:38.90 Forearm.seek CC @16:00:40.16 CC.seek :closed @16:00:44.67 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.10 @16:00:44.68 Hand.open @16:00:45.40 Forearm.reconfigure :loose @16:00:45.44 Forearm.seek :retract @16:00:46.40 collectSample 1000ml w/bubble point of 35psi @16:00:46.42 Priming 7ml @16:00:46.43 Intake.select :close @16:00:46.44 Exhaust.select :close @16:00:46.45 SS.hold @16:00:46.65 Calibrating pressure sensors @16:00:46.66 CTV.dial :air @16:00:46.68 SSV.dial :spit,avoiding: :exhaust @16:00:46.70 SS.reconfigure :fast @16:00:46.74 SS.seek 5ml @16:00:49.65 SSV.dial CBV @16:00:49.68 CBV.dial SSV @16:00:50.99 delay 3.8 seconds @16:00:54.91 SSV.dial :spit @16:00:56.21 SS.seek 0ml @16:00:59.09 SSV.dial CBV @16:01:00.41 CTV.dial :intake @16:01:01.16 SSV.dial CBV @16:01:01.19 CBV.dial SSV @16:01:01.20 Allowing 40s for 7.0ml of remaining 7.0ml @16:01:01.22 Intake.select :open @16:01:03.37 Intake @15.3psia, vacuum from 9.8 to 12.8psi @16:01:03.39 SS.reconfigure :inhaling 2.20ml/s @16:01:03.43 SS.seek 7ml @16:01:09.30 Stroke completed -- waiting for pressure to equalize ... @16:01:09.31 SS.reconfigure :exhale @16:01:09.35 SS.hold @16:01:14.90 Intake.select :close @16:01:15.04 SSV.dial :exhaust @16:01:15.78 Exhaust.select :open @16:01:15.89 SS.seek 0ml @16:01:20.16 Exhaust.select :close @16:01:20.26 Sampling 1000.0ml @16:01:20.28 Intake.select :close @16:01:20.29 Exhaust.select :close @16:01:20.30 SS.reconfigure :default @16:01:20.34 SS.hold @16:01:20.53 CTV.dial :intake @16:01:20.55 SSV.dial CBV @16:01:20.57 CBV.dial SSV @16:01:21.33 Allowing 130s for 25.0ml of remaining 1000.0ml @16:01:21.35 Intake.select :open @16:01:23.50 Intake @15.2psia, vacuum from 9.7 to 12.7psi @16:01:23.52 SS.reconfigure :inhaling 2.20ml/s @16:01:23.56 SS.seek 25ml @16:01:32.98 Vacuum too high -- waiting up to 40 seconds @16:01:32.99 SS.reconfigure :exhale @16:01:33.03 SS.hold @16:01:33.71 Intake @11.9psia, vacuum from 6.4 to 9.4psi @16:01:33.73 SS.reconfigure :inhaling 1.98ml/s @16:01:33.77 SS.seek 25ml @16:01:39.42 Vacuum too high -- waiting up to 40 seconds @16:01:39.43 SS.reconfigure :exhale @16:01:39.47 SS.hold @16:01:46.04 Intake @13.6psia, vacuum from 8.1 to 11.1psi @16:01:46.06 SS.reconfigure :inhaling 1.79ml/s @16:01:46.10 SS.seek 25ml @16:01:47.26 Stroke completed -- waiting for pressure to equalize ... @16:01:47.28 SS.reconfigure :exhale @16:01:47.32 SS.hold @16:01:47.62 Intake.select :close @16:01:47.77 SSV.dial :exhaust @16:01:48.52 Exhaust.select :open @16:01:48.63 SS.seek 0ml @16:01:57.76 Exhaust.select :close @16:01:57.87 SSV.dial CBV @16:01:57.90 CBV.dial SSV @16:01:58.64 Allowing 130s for 25.0ml of remaining 975.0ml @16:01:58.66 Intake.select :open @16:02:00.81 Intake @15.2psia, vacuum from 9.7 to 12.7psi @16:02:00.83 SS.reconfigure :inhaling 1.79ml/s @16:02:00.88 SS.seek 25ml @16:02:18.50 Stroke completed -- waiting for pressure to equalize ... @16:02:18.51 SS.reconfigure :exhale @16:02:18.56 SS.hold @16:02:24.97 Intake.select :close @16:02:25.11 SSV.dial :exhaust @16:02:25.86 Exhaust.select :open @16:02:25.97 SS.seek 0ml @16:02:35.06 Exhaust.select :close @16:02:35.17 SSV.dial CBV @16:02:35.19 CBV.dial SSV @16:02:35.95 Allowing 130s for 25.0ml of remaining 950.0ml @16:02:35.97 Intake.select :open @16:02:38.12 Intake @15.3psia, vacuum from 9.8 to 12.8psi @16:02:38.14 SS.reconfigure :inhaling 1.79ml/s @16:02:38.18 SS.seek 25ml @16:02:55.08 Stroke completed -- waiting for pressure to equalize ... @16:02:55.09 SS.reconfigure :exhale @16:02:55.13 SS.hold @16:03:01.98 Intake.select :close @16:03:02.13 SSV.dial :exhaust @16:03:02.87 Exhaust.select :open @16:03:02.98 SS.seek 0ml @16:03:12.04 Exhaust.select :close @16:03:12.15 SSV.dial CBV @16:03:12.17 CBV.dial SSV @16:03:12.94 Allowing 130s for 25.0ml of remaining 925.0ml @16:03:12.95 Intake.select :open @16:03:15.11 Intake @15.3psia, vacuum from 9.8 to 12.8psi @16:03:15.13 SS.reconfigure :inhaling 1.79ml/s @16:03:15.17 SS.seek 25ml @16:03:31.66 Vacuum too high -- waiting up to 40 seconds @16:03:31.67 SS.reconfigure :exhale @16:03:31.72 SS.hold @16:03:32.30 Intake @12.1psia, vacuum from 6.6 to 9.6psi @16:03:32.32 SS.reconfigure :inhaling 1.61ml/s @16:03:32.36 SS.seek 25ml @16:03:32.45 Vacuum too high -- waiting up to 40 seconds @16:03:32.46 SS.reconfigure :exhale @16:03:32.50 SS.hold @16:03:38.20 Intake @13.4psia, vacuum from 7.9 to 10.9psi @16:03:38.22 SS.reconfigure :inhaling 1.45ml/s @16:03:38.26 SS.seek 25ml @16:03:39.54 Stroke completed -- waiting for pressure to equalize ... @16:03:39.55 SS.reconfigure :exhale @16:03:39.60 SS.hold @16:03:39.90 Intake.select :close @16:03:40.04 SSV.dial :exhaust @16:03:40.78 Exhaust.select :open @16:03:40.89 SS.seek 0ml @16:03:49.73 Exhaust.select :close @16:03:49.84 SSV.dial CBV @16:03:49.88 CBV.dial SSV @16:03:50.69 Allowing 130s for 25.0ml of remaining 900.0ml @16:03:51.17 Intake.select :open @16:03:53.34 Intake @15.4psia, vacuum from 9.9 to 12.9psi @16:03:53.36 SS.reconfigure :inhaling 1.45ml/s @16:03:53.40 SS.seek 25ml @16:04:13.82 Stroke completed -- waiting for pressure to equalize ... @16:04:13.84 SS.reconfigure :exhale @16:04:13.88 SS.hold @16:04:20.30 Intake.select :close @16:04:20.45 SSV.dial :exhaust @16:04:21.18 Exhaust.select :open @16:04:21.30 SS.seek 0ml @16:04:30.11 Exhaust.select :close @16:04:30.21 SSV.dial CBV @16:04:30.24 CBV.dial SSV @16:04:30.98 Allowing 130s for 25.0ml of remaining 875.0ml @16:04:31.00 Intake.select :open @16:04:33.15 Intake @15.3psia, vacuum from 9.8 to 12.8psi @16:04:33.17 SS.reconfigure :inhaling 1.45ml/s @16:04:33.22 SS.seek 25ml @16:04:36.20 recoverPRVreagent08 @16:04:36.21 PTV.dial :mixing @16:04:36.97 PBV.dial :waste1 @16:04:37.35 delay 30 seconds @16:04:53.96 Stroke completed -- waiting for pressure to equalize ... @16:04:53.98 SS.reconfigure :exhale @16:04:54.02 SS.hold @16:05:00.43 Intake.select :close @16:05:00.58 SSV.dial :exhaust @16:05:01.32 Exhaust.select :open @16:05:01.44 SS.seek 0ml @16:05:07.36 PBV.dial between PSV and waste1 @16:05:07.74 PSV.dial :air @16:05:08.21 PS.reconfigure :fast @16:05:08.25 PS.seek 2.5ml @16:05:10.51 Exhaust.select :close @16:05:10.62 SSV.dial CBV @16:05:10.64 CBV.dial SSV @16:05:11.40 Allowing 130s for 25.0ml of remaining 850.0ml @16:05:11.42 Intake.select :open @16:05:13.57 Intake @15.4psia, vacuum from 9.9 to 12.9psi @16:05:13.59 SS.reconfigure :inhaling 1.45ml/s @16:05:13.63 SS.seek 25ml @16:05:32.08 PSV.dial :mixloop @16:05:32.55 delay 2 seconds @16:05:32.66 Vacuum too high -- waiting up to 40 seconds @16:05:32.67 SS.reconfigure :exhale @16:05:32.71 SS.hold @16:05:34.09 Intake @12.7psia, vacuum from 7.2 to 10.2psi @16:05:34.11 SS.reconfigure :inhaling 1.30ml/s @16:05:34.15 SS.seek 25ml @16:05:34.56 PSV.dial :air @16:05:35.03 delay 2 seconds @16:05:35.43 Stroke completed -- waiting for pressure to equalize ... @16:05:35.44 SS.reconfigure :exhale @16:05:35.49 SS.hold @16:05:37.05 PSV.dial :mixloop @16:05:37.52 delay 2 seconds @16:05:39.53 PSV.dial :air @16:05:40.01 PS.seek 0ml @16:05:41.90 Intake.select :close @16:05:42.05 SSV.dial :exhaust @16:05:43.28 Exhaust.select :open @16:05:43.39 SS.seek 0ml @16:05:52.43 Exhaust.select :close @16:05:52.54 SSV.dial CBV @16:05:52.56 CBV.dial SSV @16:05:53.31 Allowing 130s for 25.0ml of remaining 825.0ml @16:05:53.32 Intake.select :open @16:05:55.48 Intake @15.2psia, vacuum from 9.7 to 12.7psi @16:05:55.50 SS.reconfigure :inhaling 1.30ml/s @16:05:55.55 SS.seek 25ml @16:06:03.48 washCycleDA #1 of 2 @16:06:03.50 PSR.select :air @16:06:03.51 PSV.dial PSR @16:06:04.88 Pulling 0.5ml of PSR.wash @16:06:04.89 PSR.select :wash @16:06:05.01 PS.reconfigure :default @16:06:05.05 PS.seek 0.5ml @16:06:11.02 delay 5 seconds @16:06:16.04 PSR.select :air @16:06:16.15 PS.seek 2ml @16:06:17.49 Stroke completed -- waiting for pressure to equalize ... @16:06:17.51 SS.reconfigure :exhale @16:06:17.55 SS.hold @16:06:26.05 Intake.select :close @16:06:26.19 SSV.dial :exhaust @16:06:26.94 Exhaust.select :open @16:06:27.05 SS.seek 0ml @16:06:32.48 PTV.dial :puck @16:06:33.20 PSV.dial PTV @16:06:33.94 PBV.dial :waste1 @16:06:34.37 PS.seek 1.5ml @16:06:35.90 Exhaust.select :close @16:06:36.01 SSV.dial CBV @16:06:36.03 CBV.dial SSV @16:06:36.81 Allowing 130s for 25.0ml of remaining 800.0ml @16:06:36.82 Intake.select :open @16:06:38.98 Intake @15.4psia, vacuum from 9.9 to 12.9psi @16:06:39.00 SS.reconfigure :inhaling 1.30ml/s @16:06:39.04 SS.seek 25ml @16:06:40.33 delay 3 seconds @16:06:43.34 PBV.dial between PSV and waste1 @16:06:43.71 PS.seek 0ml @16:06:57.31 Vacuum too high -- waiting up to 40 seconds @16:06:57.33 SS.reconfigure :exhale @16:06:57.37 SS.hold @16:06:59.90 delay 2:00 @16:06:59.96 Intake @13.2psia, vacuum from 7.7 to 10.7psi @16:06:59.98 SS.reconfigure :inhaling 1.17ml/s @16:07:00.02 SS.seek 25ml @16:07:04.85 Stroke completed -- waiting for pressure to equalize ... @16:07:04.86 SS.reconfigure :exhale @16:07:04.91 SS.hold @16:07:10.45 Intake.select :close @16:07:10.59 SSV.dial :exhaust @16:07:11.34 Exhaust.select :open @16:07:11.45 SS.seek 0ml @16:07:20.60 Exhaust.select :close @16:07:20.71 SSV.dial CBV @16:07:20.73 CBV.dial SSV @16:07:21.48 Allowing 130s for 25.0ml of remaining 775.0ml @16:07:21.49 Intake.select :open @16:07:23.65 Intake @15.3psia, vacuum from 9.8 to 12.8psi @16:07:23.67 SS.reconfigure :inhaling 1.17ml/s @16:07:23.71 SS.seek 25ml @16:07:46.59 Vacuum too high -- waiting up to 40 seconds @16:07:46.61 SS.reconfigure :exhale @16:07:46.65 SS.hold @16:07:49.65 Intake @13.3psia, vacuum from 7.8 to 10.8psi @16:07:49.67 SS.reconfigure :inhaling 1.06ml/s @16:07:49.72 SS.seek 25ml @16:07:51.15 Stroke completed -- waiting for pressure to equalize ... @16:07:51.16 SS.reconfigure :exhale @16:07:51.20 SS.hold @16:07:56.78 Intake.select :close @16:07:56.93 SSV.dial :exhaust @16:07:57.66 Exhaust.select :open @16:07:57.78 SS.seek 0ml @16:08:06.82 Exhaust.select :close @16:08:06.93 SSV.dial CBV @16:08:06.95 CBV.dial SSV @16:08:07.70 Allowing 130s for 25.0ml of remaining 750.0ml @16:08:07.71 Intake.select :open @16:08:09.87 Intake @15.3psia, vacuum from 9.8 to 12.8psi @16:08:09.88 SS.reconfigure :inhaling 1.06ml/s @16:08:09.93 SS.seek 25ml @16:08:36.13 Stroke completed -- waiting for pressure to equalize ... @16:08:36.14 SS.reconfigure :exhale @16:08:36.19 SS.hold @16:08:44.66 Intake.select :close @16:08:44.80 SSV.dial :exhaust @16:08:45.55 Exhaust.select :open @16:08:45.66 SS.seek 0ml @16:08:54.49 Exhaust.select :close @16:08:54.60 SSV.dial CBV @16:08:54.62 CBV.dial SSV @16:08:55.40 Allowing 130s for 25.0ml of remaining 725.0ml @16:08:55.41 Intake.select :open @16:08:57.57 Intake @15.4psia, vacuum from 9.9 to 12.9psi @16:08:57.59 SS.reconfigure :inhaling 1.06ml/s @16:08:57.63 SS.seek 25ml @16:08:59.94 recoverPSRreagent08 @16:08:59.96 PTV.dial :puck @16:08:59.97 PBV.dial :waste1 @16:09:00.33 delay 30 seconds @16:09:20.53 Vacuum too high -- waiting up to 40 seconds @16:09:20.54 SS.reconfigure :exhale @16:09:20.59 SS.hold @16:09:24.77 Intake @13.8psia, vacuum from 8.3 to 11.3psi @16:09:24.78 SS.reconfigure :inhaling 0.95ml/s @16:09:24.83 SS.seek 25ml @16:09:29.43 Stroke completed -- waiting for pressure to equalize ... @16:09:29.44 SS.reconfigure :exhale @16:09:29.48 SS.hold @16:09:30.39 PBV.dial between PSV and waste1 @16:09:30.74 PSR.select :air @16:09:30.76 PSV.dial PSR @16:09:31.52 PS.reconfigure :fast @16:09:31.56 PS.seek 2.5ml @16:09:35.14 Intake.select :close @16:09:35.78 SSV.dial :exhaust @16:09:36.52 Exhaust.select :open @16:09:36.63 SS.seek 0ml @16:09:45.47 Exhaust.select :close @16:09:45.58 SSV.dial CBV @16:09:45.60 CBV.dial SSV @16:09:46.37 Allowing 130s for 25.0ml of remaining 700.0ml @16:09:46.38 Intake.select :open @16:09:48.54 Intake @15.5psia, vacuum from 10.0 to 13.0psi @16:09:48.56 SS.reconfigure :inhaling 0.95ml/s @16:09:48.61 SS.seek 25ml @16:09:55.23 PSV.dial PTV @16:09:56.00 delay 5 seconds @16:10:01.02 PSV.dial PSR @16:10:01.76 delay 5 seconds @16:10:06.78 PSV.dial PTV @16:10:07.53 delay 5 seconds @16:10:12.55 PSV.dial :air @16:10:13.30 PS.seek 0ml @16:10:17.15 Vacuum too high -- waiting up to 40 seconds @16:10:17.17 SS.reconfigure :exhale @16:10:17.21 SS.hold @16:10:22.08 Intake @13.9psia, vacuum from 8.4 to 11.4psi @16:10:22.10 SS.reconfigure :inhaling 0.87ml/s @16:10:22.14 SS.seek 25ml @16:10:23.29 Stroke completed -- waiting for pressure to equalize ... @16:10:23.30 SS.reconfigure :exhale @16:10:23.35 SS.hold @16:10:27.44 Intake.select :close @16:10:27.58 SSV.dial :exhaust @16:10:28.34 Exhaust.select :open @16:10:28.45 SS.seek 0ml @16:10:36.51 washCycleDA #2 of 2 @16:10:36.52 PSR.select :air @16:10:36.54 PSV.dial PSR @16:10:37.52 Exhaust.select :close @16:10:37.63 SSV.dial CBV @16:10:37.65 CBV.dial SSV @16:10:37.89 Pulling 0.5ml of PSR.wash @16:10:37.90 PSR.select :wash @16:10:38.01 PS.reconfigure :default @16:10:38.06 PS.seek 0.5ml @16:10:38.41 Allowing 130s for 25.0ml of remaining 675.0ml @16:10:38.43 Intake.select :open @16:10:40.58 Intake @15.4psia, vacuum from 9.9 to 12.9psi @16:10:40.60 SS.reconfigure :inhaling 0.87ml/s @16:10:40.64 SS.seek 25ml @16:10:44.04 delay 5 seconds @16:10:49.06 PSR.select :air @16:10:49.18 PS.seek 2ml @16:11:05.35 PTV.dial :puck @16:11:05.36 PSV.dial PTV @16:11:06.21 PBV.dial :waste1 @16:11:06.65 PS.seek 1.5ml @16:11:11.85 Stroke completed -- waiting for pressure to equalize ... @16:11:11.86 SS.reconfigure :exhale @16:11:11.90 SS.hold @16:11:12.60 delay 3 seconds @16:11:15.62 PBV.dial between PSV and waste1 @16:11:16.00 PS.seek 0ml @16:11:21.61 Intake.select :close @16:11:21.75 SSV.dial :exhaust @16:11:22.50 Exhaust.select :open @16:11:22.61 SS.seek 0ml @16:11:31.63 Exhaust.select :close @16:11:31.74 SSV.dial CBV @16:11:32.23 CBV.dial SSV @16:11:32.38 delay 2:00 @16:11:33.01 Allowing 130s for 25.0ml of remaining 650.0ml @16:11:33.02 Intake.select :open @16:11:35.17 Intake @15.5psia, vacuum from 10.0 to 13.0psi @16:11:35.19 SS.reconfigure :inhaling 0.87ml/s @16:11:35.24 SS.seek 25ml @16:12:01.90 Vacuum too high -- waiting up to 40 seconds @16:12:01.91 SS.reconfigure :exhale @16:12:01.96 SS.hold @16:12:06.14 Intake @13.8psia, vacuum from 8.3 to 11.3psi @16:12:06.16 SS.reconfigure :inhaling 0.78ml/s @16:12:06.20 SS.seek 25ml @16:12:12.41 Stroke completed -- waiting for pressure to equalize ... @16:12:12.42 SS.reconfigure :exhale @16:12:12.46 SS.hold @16:12:19.86 Intake.select :close @16:12:20.00 SSV.dial :exhaust @16:12:20.75 Exhaust.select :open @16:12:20.86 SS.seek 0ml @16:12:29.67 Exhaust.select :close @16:12:29.78 SSV.dial CBV @16:12:29.80 CBV.dial SSV @16:12:30.57 Allowing 130s for 25.0ml of remaining 625.0ml @16:12:30.58 Intake.select :open @16:12:32.73 Intake @15.4psia, vacuum from 9.9 to 12.9psi @16:12:32.75 SS.reconfigure :inhaling 0.78ml/s @16:12:32.80 SS.seek 25ml @16:13:07.09 Vacuum too high -- waiting up to 40 seconds @16:13:07.10 SS.reconfigure :exhale @16:13:07.14 SS.hold @16:13:12.79 Intake @14.2psia, vacuum from 8.7 to 11.7psi @16:13:12.81 SS.reconfigure :inhaling 0.70ml/s @16:13:12.85 SS.seek 25ml @16:13:13.97 Stroke completed -- waiting for pressure to equalize ... @16:13:13.98 SS.reconfigure :exhale @16:13:14.02 SS.hold @16:13:20.44 Intake.select :close @16:13:20.58 SSV.dial :exhaust @16:13:21.33 Exhaust.select :open @16:13:21.44 SS.seek 0ml @16:13:30.28 Exhaust.select :close @16:13:30.39 SSV.dial CBV @16:13:30.41 CBV.dial SSV @16:13:31.17 Allowing 130s for 25.0ml of remaining 600.0ml @16:13:31.19 Intake.select :open @16:13:32.40 recoverPSRreagent08 @16:13:32.41 PTV.dial :puck @16:13:32.43 PBV.dial :waste1 @16:13:32.79 delay 30 seconds @16:13:33.34 Intake @15.4psia, vacuum from 9.9 to 12.9psi @16:13:33.36 SS.reconfigure :inhaling 0.70ml/s @16:13:33.40 SS.seek 25ml @16:14:02.81 PBV.dial between PSV and waste1 @16:14:03.18 PSR.select :air @16:14:03.20 PSV.dial PSR @16:14:04.41 PS.reconfigure :fast @16:14:04.47 PS.seek 2.5ml @16:14:11.53 Stroke completed -- waiting for pressure to equalize ... @16:14:11.54 SS.reconfigure :exhale @16:14:11.59 SS.hold @16:14:24.16 Intake.select :close @16:14:24.30 SSV.dial :exhaust @16:14:25.05 Exhaust.select :open @16:14:25.16 SS.seek 0ml @16:14:28.32 PSV.dial PTV @16:14:29.07 delay 5 seconds @16:14:34.06 Exhaust.select :close @16:14:34.11 PSV.dial PSR @16:14:34.17 SSV.dial CBV @16:14:34.19 CBV.dial SSV @16:14:34.87 delay 5 seconds @16:14:34.96 Allowing 130s for 25.0ml of remaining 575.0ml @16:14:34.97 Intake.select :open @16:14:37.12 Intake @15.4psia, vacuum from 9.9 to 12.9psi @16:14:37.14 SS.reconfigure :inhaling 0.70ml/s @16:14:37.18 SS.seek 25ml @16:14:39.88 PSV.dial PTV @16:14:40.63 delay 5 seconds @16:14:45.65 PSV.dial :air @16:14:46.53 PS.seek 0ml @16:15:05.93 Vacuum too high -- waiting up to 40 seconds @16:15:05.94 SS.reconfigure :exhale @16:15:05.98 SS.hold @16:15:09.63 getPMLReagent08 @16:15:09.64 PRV.dial :air @16:15:10.13 delay 5 seconds @16:15:11.63 Intake @14.4psia, vacuum from 8.9 to 11.9psi @16:15:11.65 SS.reconfigure :inhaling 0.63ml/s @16:15:11.69 SS.seek 25ml @16:15:15.15 PTV.dial :PRVmixing @16:15:16.46 delay 5 seconds @16:15:21.48 PRV.dial :ab2 @16:15:21.92 PTV.dial :PRVmixing @16:15:21.94 PSV.dial :mixloop @16:15:22.38 PS.reconfigure :default @16:15:22.43 PS.seek 0.3ml @16:15:22.51 Stroke completed -- waiting for pressure to equalize ... @16:15:22.53 SS.reconfigure :exhale @16:15:22.57 SS.hold @16:15:26.31 delay 2 seconds @16:15:28.33 PRV.dial :air @16:15:28.86 PS.seek 0.55ml @16:15:32.19 addPMLReagent08 @16:15:32.20 PSV.dial :air @16:15:32.40 Intake.select :close @16:15:32.54 SSV.dial :exhaust @16:15:32.67 PS.seek 2ml @16:15:33.28 Exhaust.select :open @16:15:33.39 SS.seek 0ml @16:15:42.26 Exhaust.select :close @16:15:42.37 SSV.dial CBV @16:15:42.39 CBV.dial SSV @16:15:43.16 Allowing 130s for 25.0ml of remaining 550.0ml @16:15:43.17 Intake.select :open @16:15:45.32 Intake @15.4psia, vacuum from 9.9 to 12.9psi @16:15:45.76 SS.reconfigure :inhaling 0.63ml/s @16:15:45.88 SS.seek 25ml @16:15:48.29 PSV.dial :PML @16:15:48.75 PTV.dial :mixing @16:15:49.50 PBV.dial :waste1 @16:15:49.94 PS.seek 1.85ml @16:15:52.47 PBV.dial between PSV and waste1 @16:15:52.86 PS.seek 0ml @16:16:12.53 delay 2 seconds @16:16:14.55 delay 8:00 @16:16:19.37 Vacuum too high -- waiting up to 40 seconds @16:16:19.38 SS.reconfigure :exhale @16:16:19.43 SS.hold @16:16:25.82 Intake @14.4psia, vacuum from 8.9 to 11.9psi @16:16:25.83 SS.reconfigure :inhaling 0.57ml/s @16:16:25.88 SS.seek 25ml @16:16:36.03 Stroke completed -- waiting for pressure to equalize ... @16:16:36.04 SS.reconfigure :exhale @16:16:36.08 SS.hold @16:16:47.12 Intake.select :close @16:16:47.26 SSV.dial :exhaust @16:16:48.01 Exhaust.select :open @16:16:48.12 SS.seek 0ml @16:16:56.92 Exhaust.select :close @16:16:57.02 SSV.dial CBV @16:16:57.08 CBV.dial SSV @16:16:57.78 Allowing 130s for 25.0ml of remaining 525.0ml @16:16:57.79 Intake.select :open @16:16:59.95 Intake @15.6psia, vacuum from 10.0 to 13.0psi @16:16:59.97 SS.reconfigure :inhaling 0.57ml/s @16:17:00.01 SS.seek 25ml @16:17:36.55 Vacuum too high -- waiting up to 40 seconds @16:17:36.56 SS.reconfigure :exhale @16:17:36.61 SS.hold @16:17:43.01 Intake @14.7psia, vacuum from 9.2 to 12.2psi @16:17:43.03 SS.reconfigure :inhaling 0.51ml/s @16:17:43.07 SS.seek 25ml @16:17:54.70 Stroke completed -- waiting for pressure to equalize ... @16:17:54.71 SS.reconfigure :exhale @16:17:54.75 SS.hold @16:18:05.88 Intake.select :close @16:18:06.02 SSV.dial :exhaust @16:18:06.76 Exhaust.select :open @16:18:06.87 SS.seek 0ml @16:18:16.24 Exhaust.select :close @16:18:16.36 SSV.dial CBV @16:18:16.38 CBV.dial SSV @16:18:17.14 Allowing 130s for 25.0ml of remaining 500.0ml @16:18:17.15 Intake.select :open @16:18:19.30 Intake @15.6psia, vacuum from 10.0 to 13.0psi @16:18:19.32 SS.reconfigure :inhaling 0.51ml/s @16:18:19.37 SS.seek 25ml @16:18:58.83 Vacuum too high -- waiting up to 40 seconds @16:18:58.84 SS.reconfigure :exhale @16:18:58.89 SS.hold @16:19:04.88 Intake @14.8psia, vacuum from 9.3 to 12.3psi @16:19:04.90 SS.reconfigure :inhaling 0.47ml/s @16:19:04.94 SS.seek 25ml @16:19:18.90 Stroke completed -- waiting for pressure to equalize ... @16:19:18.91 SS.reconfigure :exhale @16:19:18.95 SS.hold @16:19:31.54 Intake.select :close @16:19:31.68 SSV.dial :exhaust @16:19:32.42 Exhaust.select :open @16:19:32.53 SS.seek 0ml @16:19:41.37 Exhaust.select :close @16:19:41.48 SSV.dial CBV @16:19:41.53 CBV.dial SSV @16:19:42.25 Allowing 130s for 25.0ml of remaining 475.0ml @16:19:42.26 Intake.select :open @16:19:44.41 Intake @15.4psia, vacuum from 9.9 to 12.9psi @16:19:44.43 SS.reconfigure :inhaling 0.47ml/s @16:19:44.47 SS.seek 25ml @16:20:35.11 Vacuum too high -- waiting up to 40 seconds @16:20:35.12 SS.reconfigure :exhale @16:20:35.16 SS.hold @16:20:43.73 Intake @14.7psia, vacuum from 9.2 to 12.2psi @16:20:43.75 SS.reconfigure :inhaling 0.43ml/s @16:20:43.79 SS.seek 25ml @16:20:51.55 Stroke completed -- waiting for pressure to equalize ... @16:20:51.56 SS.reconfigure :exhale @16:20:51.61 SS.hold @16:21:02.65 Intake.select :close @16:21:02.79 SSV.dial :exhaust @16:21:03.54 Exhaust.select :open @16:21:03.65 SS.seek 0ml @16:21:12.47 Exhaust.select :close @16:21:13.08 SSV.dial CBV @16:21:13.12 CBV.dial SSV @16:21:13.87 Allowing 130s for 25.0ml of remaining 450.0ml @16:21:13.88 Intake.select :open @16:21:16.04 Intake @15.5psia, vacuum from 10.0 to 13.0psi @16:21:16.06 SS.reconfigure :inhaling 0.43ml/s @16:21:16.10 SS.seek 25ml @16:22:19.36 Vacuum too high -- waiting up to 40 seconds @16:22:19.37 SS.reconfigure :exhale @16:22:19.41 SS.hold @16:22:27.98 Intake @14.8psia, vacuum from 9.3 to 12.3psi @16:22:28.00 SS.reconfigure :inhaling 0.38ml/s @16:22:28.04 SS.seek 25ml @16:22:30.14 Stroke completed -- waiting for pressure to equalize ... @16:22:30.15 SS.reconfigure :exhale @16:22:30.19 SS.hold @16:22:41.27 Intake.select :close @16:22:41.41 SSV.dial :exhaust @16:22:42.16 Exhaust.select :open @16:22:42.27 SS.seek 0ml @16:22:51.16 Exhaust.select :close @16:22:51.27 SSV.dial CBV @16:22:51.29 CBV.dial SSV @16:22:52.05 Allowing 130s for 25.0ml of remaining 425.0ml @16:22:52.07 Intake.select :open @16:22:54.22 Intake @15.5psia, vacuum from 10.0 to 13.0psi @16:22:54.24 SS.reconfigure :inhaling 0.38ml/s @16:22:54.28 SS.seek 25ml @16:24:11.81 Stroke completed -- waiting for pressure to equalize ... @16:24:11.83 SS.reconfigure :exhale @16:24:11.87 SS.hold @16:24:14.57 recoverPRVreagent08 @16:24:14.58 PTV.dial :mixing @16:24:14.59 PBV.dial :waste1 @16:24:14.96 delay 30 seconds @16:24:32.18 Intake.select :close @16:24:32.32 SSV.dial :exhaust @16:24:33.07 Exhaust.select :open @16:24:33.18 SS.seek 0ml @16:24:42.25 Exhaust.select :close @16:24:42.36 SSV.dial CBV @16:24:42.38 CBV.dial SSV @16:24:43.15 Allowing 130s for 25.0ml of remaining 400.0ml @16:24:43.16 Intake.select :open @16:24:44.98 PBV.dial between PSV and waste1 @16:24:45.32 Intake @15.4psia, vacuum from 9.9 to 12.9psi @16:24:45.35 SS.reconfigure :inhaling 0.38ml/s @16:24:45.39 SS.seek 25ml @16:24:45.45 PSV.dial :air @16:24:45.92 PS.reconfigure :fast @16:24:45.96 PS.seek 2.5ml @16:25:09.86 PSV.dial :mixloop @16:25:10.84 delay 2 seconds @16:25:12.85 PSV.dial :air @16:25:13.32 delay 2 seconds @16:25:15.34 PSV.dial :mixloop @16:25:15.81 delay 2 seconds @16:25:17.82 PSV.dial :air @16:25:18.29 PS.seek 0ml @16:25:41.43 washCycleDA #1 of 3 @16:25:41.44 PSR.select :air @16:25:41.45 PSV.dial PSR @16:25:42.81 Pulling 0.5ml of PSR.wash @16:25:42.82 PSR.select :wash @16:25:42.93 PS.reconfigure :default @16:25:42.98 PS.seek 0.5ml @16:25:48.99 delay 5 seconds @16:25:54.01 PSR.select :air @16:25:54.13 PS.seek 2ml @16:26:01.98 Stroke completed -- waiting for pressure to equalize ... @16:26:02.00 SS.reconfigure :exhale @16:26:02.04 SS.hold @16:26:10.21 PTV.dial :puck @16:26:10.94 PSV.dial PTV @16:26:11.80 PBV.dial :waste1 @16:26:12.24 PS.seek 1.5ml @16:26:18.20 delay 3 seconds @16:26:21.22 PBV.dial between PSV and waste1 @16:26:21.61 PS.seek 0ml @16:26:26.41 Intake.select :close @16:26:26.56 SSV.dial :exhaust @16:26:27.30 Exhaust.select :open @16:26:27.41 SS.seek 0ml @16:26:36.41 Exhaust.select :close @16:26:36.51 SSV.dial CBV @16:26:36.54 CBV.dial SSV @16:26:37.28 Allowing 130s for 25.0ml of remaining 375.0ml @16:26:37.30 Intake.select :open @16:26:37.68 delay 2:00 @16:26:39.45 Intake @15.4psia, vacuum from 9.9 to 12.9psi @16:26:39.47 SS.reconfigure :inhaling 0.38ml/s @16:26:39.51 SS.seek 25ml @16:27:37.73 Vacuum too high -- waiting up to 40 seconds @16:27:37.74 SS.reconfigure :exhale @16:27:37.78 SS.hold @16:27:50.45 Intake @14.9psia, vacuum from 9.4 to 12.4psi @16:27:50.47 SS.reconfigure :inhaling 0.34ml/s @16:27:50.51 SS.seek 25ml @16:28:12.00 Stroke completed -- waiting for pressure to equalize ... @16:28:12.01 SS.reconfigure :exhale @16:28:12.05 SS.hold @16:28:32.96 Intake.select :close @16:28:33.10 SSV.dial :exhaust @16:28:33.84 Exhaust.select :open @16:28:33.96 SS.seek 0ml @16:28:37.70 recoverPSRreagent08 @16:28:37.71 PTV.dial :puck @16:28:37.72 PBV.dial :waste1 @16:28:38.08 delay 30 seconds @16:28:42.73 Exhaust.select :close @16:28:42.85 SSV.dial CBV @16:28:42.87 CBV.dial SSV @16:28:43.63 Allowing 130s for 25.0ml of remaining 350.0ml @16:28:43.64 Intake.select :open @16:28:45.79 Intake @15.5psia, vacuum from 10.0 to 13.0psi @16:28:45.81 SS.reconfigure :inhaling 0.34ml/s @16:28:45.86 SS.seek 25ml @16:29:08.11 PBV.dial between PSV and waste1 @16:29:08.46 PSR.select :air @16:29:08.47 PSV.dial PSR @16:29:09.21 PS.reconfigure :fast @16:29:09.26 PS.seek 2.5ml @16:29:32.92 PSV.dial PTV @16:29:33.80 delay 5 seconds @16:29:38.82 PSV.dial PSR @16:29:39.55 delay 5 seconds @16:29:44.57 PSV.dial PTV @16:29:45.32 delay 5 seconds @16:29:50.34 PSV.dial :air @16:29:51.21 PS.seek 0ml @16:30:05.47 Vacuum too high -- waiting up to 40 seconds @16:30:05.49 SS.reconfigure :exhale @16:30:05.53 SS.hold @16:30:14.36 washCycleDA #2 of 3 @16:30:14.37 PSR.select :air @16:30:14.39 PSV.dial PSR @16:30:15.76 Pulling 0.5ml of PSR.wash @16:30:15.77 PSR.select :wash @16:30:15.88 PS.reconfigure :default @16:30:15.92 PS.seek 0.5ml @16:30:18.20 Intake @15.0psia, vacuum from 9.5 to 12.5psi @16:30:18.22 SS.reconfigure :inhaling 0.31ml/s @16:30:18.26 SS.seek 25ml @16:30:21.86 delay 5 seconds @16:30:26.88 PSR.select :air @16:30:27.00 PS.seek 2ml @16:30:30.57 Stroke completed -- waiting for pressure to equalize ... @16:30:30.58 SS.reconfigure :exhale @16:30:30.63 SS.hold @16:30:43.26 PTV.dial :puck @16:30:43.28 PSV.dial PTV @16:30:44.03 PBV.dial :waste1 @16:30:44.45 PS.seek 1.5ml @16:30:50.36 delay 3 seconds @16:30:53.06 Intake.select :close @16:30:53.20 SSV.dial :exhaust @16:30:53.37 PBV.dial between PSV and waste1 @16:30:53.74 PS.seek 0ml @16:30:53.95 Exhaust.select :open @16:30:54.06 SS.seek 0ml @16:31:02.87 Exhaust.select :close @16:31:03.48 SSV.dial CBV @16:31:03.50 CBV.dial SSV @16:31:04.25 Allowing 130s for 25.0ml of remaining 325.0ml @16:31:04.26 Intake.select :open @16:31:06.42 Intake @15.5psia, vacuum from 10.0 to 13.0psi @16:31:06.44 SS.reconfigure :inhaling 0.31ml/s @16:31:06.48 SS.seek 25ml @16:31:09.92 delay 2:00 @16:32:45.00 Vacuum too high -- waiting up to 40 seconds @16:32:45.01 SS.reconfigure :exhale @16:32:45.06 SS.hold @16:33:01.40 Intake @15.1psia, vacuum from 9.6 to 12.6psi @16:33:01.42 SS.reconfigure :inhaling 0.28ml/s @16:33:01.46 SS.seek 25ml @16:33:05.04 Stroke completed -- waiting for pressure to equalize ... @16:33:05.05 SS.reconfigure :exhale @16:33:05.09 SS.hold @16:33:09.94 recoverPSRreagent08 @16:33:09.95 PTV.dial :puck @16:33:09.96 PBV.dial :waste1 @16:33:10.31 delay 30 seconds @16:33:14.27 SS.reconfigure :default @16:33:14.31 SS.hold @16:33:14.43 Intake.select :close @16:33:14.57 ShallowSampler::Clogged in WCR -- Filtering too Slowly after 700.0ml @16:33:14.59 SSV.dial :exhaust @16:33:15.34 Exhaust.select :open @16:33:15.45 SS.reconfigure :exhale @16:33:15.50 SS.seek 0ml @16:33:24.48 Exhaust.select :close @16:33:24.58 Sampled 700.0ml @16:33:24.60 Drying puck before fixing for 20:00 @16:33:24.61 CBV.dial :waste2b @16:33:25.36 delay 1:00 @16:33:40.33 PBV.dial between PSV and waste1 @16:33:40.69 PSR.select :air @16:33:40.70 PSV.dial PSR @16:33:41.46 PS.reconfigure :fast @16:33:41.50 PS.seek 2.5ml @16:34:05.23 PSV.dial PTV @16:34:06.11 delay 5 seconds @16:34:11.13 PSV.dial PSR @16:34:11.86 delay 5 seconds @16:34:16.88 PSV.dial PTV @16:34:17.62 delay 5 seconds @16:34:22.64 PSV.dial :air @16:34:23.39 PS.seek 0ml @16:34:25.38 CSV.dial :air @16:34:25.84 CS.reconfigure :fast @16:34:25.88 CS.seek 10ml @16:34:46.48 washCycleDA #3 of 3 @16:34:46.49 PSR.select :air @16:34:46.51 PSV.dial PSR @16:34:47.86 Pulling 0.5ml of PSR.wash @16:34:47.87 PSR.select :wash @16:34:47.98 PS.reconfigure :default @16:34:48.03 PS.seek 0.5ml @16:34:50.14 Pumping wcr puck from tube 2 up to 35psi... @16:34:50.16 CBV.dial SSV @16:34:51.46 CBV.dial between CSV and waste2b @16:34:52.41 CSV.dial between air and CTV @16:34:52.73 CS.seek 7.324ml @16:34:53.98 delay 5 seconds @16:34:59.00 PSR.select :air @16:34:59.11 PS.seek 2ml @16:34:59.59 CTV.dial CSV,avoiding: :air @16:35:00.11 CSV.dial CTV @16:35:00.91 CS.reconfigure :pressurize @16:35:00.96 CS.seek 0ml @16:35:15.23 PTV.dial :puck @16:35:15.24 PSV.dial PTV @16:35:16.09 PBV.dial :waste1 @16:35:16.53 PS.seek 1.5ml @16:35:17.41 CBV.dial :waste2b @16:35:17.74 CS.reconfigure :default @16:35:17.79 CS.seek 0ml @16:35:21.58 delay 1:00 @16:35:22.54 delay 3 seconds @16:35:25.56 PBV.dial between PSV and waste1 @16:35:25.94 PS.seek 0ml @16:35:42.07 delay 2:00 @16:36:21.61 CTV.dial :air,avoiding: :intake @16:36:22.93 Fix #1 of 2 (with RNAlater) for 10:00 @16:36:22.94 CSV.dial CSR @16:36:23.43 Pulling 2ml of CSR.RNAlater @16:36:23.44 CSR.select :RNAlater @16:36:23.56 CS.seek 2ml @16:36:29.52 delay 5 seconds @16:36:34.54 CSR.select :air @16:36:34.65 CS.reconfigure :fast @16:36:34.69 CS.seek 10ml @16:36:54.14 Pumping wcr puck from tube 2 up to 35psi... @16:36:54.16 CBV.dial SSV @16:36:55.44 CBV.dial between CSV and waste2b @16:36:56.39 CSV.dial between air and CTV @16:36:57.00 CS.seek 7.174ml @16:37:04.15 CTV.dial CSV,avoiding: :air @16:37:04.17 CSV.dial CTV @16:37:05.55 CS.reconfigure :pressurize @16:37:05.60 CS.seek 0ml @16:37:23.44 delay 10:00 @16:37:42.10 recoverPSRreagent08 @16:37:42.11 PTV.dial :puck @16:37:42.12 PBV.dial :waste1 @16:37:42.49 delay 30 seconds @16:38:12.52 PBV.dial between PSV and waste1 @16:38:12.88 PSR.select :air @16:38:12.90 PSV.dial PSR @16:38:13.65 PS.reconfigure :fast @16:38:13.70 PS.seek 2.5ml @16:38:37.41 PSV.dial PTV @16:38:38.29 delay 5 seconds @16:38:43.31 PSV.dial PSR @16:38:44.04 delay 5 seconds @16:38:49.06 PSV.dial PTV @16:38:49.80 delay 5 seconds @16:38:54.82 PSV.dial :air @16:38:55.58 PS.seek 0ml @16:39:18.66 Gate.power :camera,:ON @16:39:18.68 Mixing DA Substrates PSR.select :air @16:39:18.70 PSV.dial PSR @16:39:20.20 Pulling 0.25ml of PSR.sub1 @16:39:20.23 PSR.select :sub1 @16:39:20.43 PS.reconfigure :default @16:39:20.57 PS.seek 0.25ml @16:39:24.02 delay 5 seconds @16:39:29.09 Pulling 0.25ml of PSR.sub2 @16:39:29.10 PSR.select :sub2 @16:39:29.21 PS.seek 0.5ml @16:39:32.57 delay 5 seconds @16:39:37.59 PSR.select :air @16:39:37.70 PS.seek 2ml @16:39:53.78 PTV.dial :puck @16:39:53.80 PSV.dial PTV @16:39:54.54 PBV.dial :waste1 @16:39:54.97 PS.seek 1.5ml @16:40:00.85 delay 3 seconds @16:40:03.87 PBV.dial between PSV and waste1 @16:40:04.23 PS.seek 0ml @16:40:20.35 delay 10 seconds @16:40:30.38 recoverPSRreagent08 @16:40:30.39 PTV.dial :puck @16:40:30.40 PBV.dial :waste1 @16:40:30.76 delay 30 seconds @16:41:00.78 PBV.dial between PSV and waste1 @16:41:01.13 PSR.select :air @16:41:01.15 PSV.dial PSR @16:41:01.92 PS.reconfigure :fast @16:41:01.96 PS.seek 2.5ml @16:41:25.94 PSV.dial PTV @16:41:26.71 delay 5 seconds @16:41:31.73 PSV.dial PSR @16:41:32.47 delay 5 seconds @16:41:37.49 PSV.dial PTV @16:41:38.25 delay 5 seconds @16:41:43.27 PSV.dial :air @16:41:44.03 PS.seek 0ml @16:42:07.57 Additional air clear @16:42:07.58 PSR.select :air @16:42:07.59 PSV.dial PSR @16:42:09.46 Pulling 2ml of PSR.air @16:42:09.47 PSR.select :air @16:42:09.50 PS.reconfigure :default @16:42:09.54 PS.seek 2ml @16:42:30.81 delay 5 seconds @16:42:35.83 PTV.dial :puck @16:42:35.85 PSV.dial PTV @16:42:36.60 PBV.dial :waste1 @16:42:37.02 PS.seek 1.5ml @16:42:42.97 delay 3 seconds @16:42:45.99 PBV.dial between PSV and waste1 @16:42:46.35 delay 1 second @16:42:47.37 PSV.dial :waste1 @16:42:48.43 PS.seek 0ml @16:43:04.61 Processing Puck Heater at 28C before imaging @16:43:04.62 PH.coast @16:43:04.75 Imaging da puck in Processing Clamp while storing it to tube 1 @16:43:04.91 SC.reconfigure :tight @16:43:04.96 SC.seek 1 @16:43:08.59 SC.configure :limp @16:43:08.79 Elbow.reconfigure :tight @16:43:08.83 Elbow.seek 1 @16:43:10.62 Forearm.reconfigure :tight @16:43:10.67 Forearm.seek 1 + 450 counts @16:43:11.69 SE.reconfigure :pick @16:43:11.74 SE.seek :beamBreak @16:43:14.07 SE.configure :default @16:43:14.21 Elbow.reconfigure :limp @16:43:14.26 Forearm.reconfigure :loose @16:43:14.30 Forearm.seek 1 @16:43:14.57 Elbow.hold @16:43:14.63 Elbow.reconfigure :tight @16:43:14.72 SE.seek 13.1 Pucks below Top Plate @16:43:15.17 Forearm.force -20 @16:43:15.20 Forearm.seek 1 + 299 counts @16:43:15.50 Forearm.reconfigure :tight @16:43:15.54 Forearm.seek 1 + 450 counts @16:43:15.75 SE.seek 14.1 Pucks below Top Plate @16:43:16.40 Forearm.reconfigure :loose @16:43:16.44 Forearm.seek :retract @16:43:17.37 Elbow.seek PC @16:43:20.04 Elbow.reconfigure :loose @16:43:20.13 Forearm.seek PC @16:43:21.10 Hand.close @16:43:21.68 PC.seek :open @16:43:26.44 Forearm.seek :retract @16:43:27.40 Elbow.reconfigure :tight @16:43:27.45 Elbow.seek 1 @16:43:29.99 Forearm.reconfigure :tight @16:43:30.04 Forearm.seek 1 + 450 counts @16:43:30.97 Hand.open @16:43:31.61 SE.seek 13.75 Pucks below Top Plate @16:43:31.86 SE.reconfigure :seal @16:43:31.90 SE.seek 12.79 Pucks below Top Plate @16:43:32.47 Lights, camera, ACTION! @16:43:32.51 PuckCamera: snap -bin=2,2 -auto /var/log/esp/da22nov2216h900ml.tif @16:43:51.06 SX HX9/16 (USB:04): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15.272 seconds /var/log/esp/da22nov2216h900ml.tif: TIFF Upload Complete @16:43:51.09 PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da22nov2216h900ml15s.tif @16:43:51.95 Waiting >20 seconds for da to relinquish FlushPuck @16:44:06.70 SX HX9/16 (USB:04): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15 seconds /var/log/esp/midres/da22nov2216h900ml15s.tif: TIFF Upload Complete @16:44:06.73 PuckCamera: snap -bin=2,2 20.0 /var/log/esp/midres/da22nov2216h900ml20s.tif @16:44:27.39 SX HX9/16 (USB:04): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 20 seconds /var/log/esp/midres/da22nov2216h900ml20s.tif: TIFF Upload Complete @16:44:27.40 Gate.power :camera,:OFF @16:44:27.42 DAfans.email "15.272 sec auto-exposure",Subject: "da22nov2216h900ml images!" @16:44:27.67 Elbow.seek 1 @16:44:27.88 Forearm.seek 1 + 450 counts @16:44:27.95 SE.reconfigure :gently @16:44:28.02 SE.seek 13.51 Pucks below Top Plate @16:44:28.76 Elbow.reconfigure :limp @16:44:28.81 Forearm.reconfigure :loose @16:44:28.86 Forearm.seek 1 @16:44:29.16 Elbow.hold @16:44:29.83 Elbow.reconfigure :tight @16:44:29.88 SE.reconfigure :default @16:44:29.92 SE.seek 13.69 Pucks below Top Plate @16:44:30.37 Forearm.force -20 @16:44:30.41 Forearm.seek 1 + 293 counts @16:44:30.72 Forearm.reconfigure :tight @16:44:30.77 Forearm.seek 1 + 450 counts @16:44:31.04 SE.seek :down @16:44:32.17 Forearm.reconfigure :loose @16:44:32.22 Forearm.seek :retract @16:44:32.88 SE.stop @16:44:33.22 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @16:44:33.44 Elbow.seek FlushPuck::Garage @16:44:35.39 Elbow.reconfigure :loose @16:44:35.58 Forearm.seek :garage @16:44:36.58 Hand.close @16:44:37.44 Forearm.seek :retract @16:44:38.48 Elbow.reconfigure :tight @16:44:38.59 Elbow.seek PC @16:44:39.97 Elbow.reconfigure :loose @16:44:40.20 Forearm.reconfigure :tight @16:44:40.28 Forearm.seek PC @16:44:41.87 PC.seek :closed @16:44:46.64 Processing Clamp onSpring/offSpring for 16/26 samples, on/off torque 1.59 @16:44:46.79 Hand.open @16:44:47.60 Forearm.reconfigure :loose @16:44:47.71 Forearm.seek :retract @16:44:48.69 PSR.select :air @16:44:48.73 PSV.dial PSR @16:44:49.39 Pulling 0.5ml of PSR.flush @16:44:49.42 PSR.select :flush @16:44:49.58 PS.seek 0.5ml @16:44:55.56 delay 5 seconds @16:45:00.60 PSR.select :air @16:45:00.79 PS.seek 2.5ml @16:45:01.45 upsync @16:45:22.00 delay 1 second @16:45:23.02 PRV.dial :waste2,avoiding: :ab2 @16:45:24.87 PTV.dial :PRVmixing @16:45:26.17 PSV.dial :mixloop @16:45:27.32 PS.seek :empty @16:45:53.81 PSR.select :air @16:45:53.83 PSV.dial PSR @16:45:54.91 Pulling 0.5ml of PSR.flush @16:45:54.92 PSR.select :flush @16:45:55.04 PS.seek 0.5ml @16:46:01.03 delay 5 seconds @16:46:06.05 PSR.select :air @16:46:06.17 PS.seek 2.5ml @16:46:27.40 delay 1 second @16:46:28.42 PBV.dial :waste1 @16:46:28.78 PTV.dial :mixing @16:46:29.53 PSV.dial :mixloop @16:46:30.58 PS.seek :empty @16:46:56.97 PSV.dial PSR @16:46:58.00 PSR.select :flush @16:46:58.11 PS.seek 1ml @16:47:09.21 PSR.select :air @16:47:09.33 PS.seek 2.5ml @16:47:23.46 CBV.dial :waste2b @16:47:23.79 CS.reconfigure :default @16:47:23.84 CS.seek 0ml @16:47:25.49 PSV.dial PTV @16:47:25.56 delay 1:00 @16:47:26.25 PTV.dial :puck @16:47:26.98 PBV.dial :waste1 @16:47:27.00 PS.seek 0ml @16:47:53.37 PSV.dial PSR @16:47:54.12 PSR.select :flush @16:47:54.75 PS.seek 0.5ml @16:48:00.65 PSR.select :air @16:48:00.76 PS.seek 2.5ml @16:48:22.06 PSV.dial PTV @16:48:22.81 PBV.dial PSV @16:48:23.38 PS.seek 0ml @16:48:25.58 CTV.dial :air,avoiding: :intake @16:48:26.90 Fix #2 of 2 (with RNAlater) for 10:00 @16:48:26.91 CSV.dial CSR @16:48:27.51 Pulling 2ml of CSR.RNAlater @16:48:27.52 CSR.select :RNAlater @16:48:27.64 CS.seek 2ml @16:48:33.54 delay 5 seconds @16:48:38.56 CSR.select :air @16:48:38.67 CS.reconfigure :fast @16:48:38.72 CS.seek 10ml @16:48:49.71 PSV.dial PBV @16:48:50.19 PS.seek 2.5ml @16:48:58.23 Pumping wcr puck from tube 2 up to 35psi... @16:48:58.24 CBV.dial SSV @16:48:59.52 CBV.dial between CSV and waste2b @16:49:00.47 CSV.dial between air and CTV @16:49:01.06 CS.seek 7.162ml @16:49:08.35 CTV.dial CSV,avoiding: :air @16:49:08.38 CSV.dial CTV @16:49:09.76 CS.reconfigure :pressurize @16:49:09.81 CS.seek 0ml @16:49:16.49 PSV.dial :waste1 @16:49:17.23 PS.seek 0ml @16:49:26.90 delay 10:00 @16:49:43.62 PSV.dial PSR @16:49:44.09 PSR.select :air @16:49:44.11 PS.seek 2.5ml @16:50:10.43 PSV.dial PTV @16:50:11.16 PBV.dial PSV @16:50:11.18 PS.seek 0ml @16:50:37.52 PSV.dial PBV @16:50:38.00 PS.seek 2.5ml @16:51:04.33 PSV.dial :waste1 @16:51:05.08 PS.seek 0ml @16:51:31.46 PSV.dial PSR @16:51:31.93 PSR.select :flush @16:51:32.05 PS.seek 1ml @16:51:43.05 PSR.select :air @16:51:43.17 PS.seek 2.5ml @16:51:59.30 PSV.dial PTV @16:52:00.04 PTV.dial :puck @16:52:00.05 PBV.dial :waste1 @16:52:00.61 PS.seek 0ml @16:52:26.99 PSV.dial PSR @16:52:27.74 PSR.select :flush @16:52:27.86 PS.seek 0.5ml @16:52:33.83 PSR.select :air @16:52:33.95 PS.seek 2.5ml @16:52:55.24 PSV.dial PTV @16:52:56.11 PBV.dial PSV @16:52:56.69 PS.seek 0ml @16:53:23.02 PSV.dial PBV @16:53:23.48 PS.seek 2.5ml @16:53:49.86 PSV.dial :waste1 @16:53:50.60 PS.seek 0ml @16:54:16.99 PSV.dial PSR @16:54:17.46 PSR.select :air @16:54:17.48 PS.seek 2.5ml @16:54:43.80 PSV.dial PTV @16:54:44.53 PBV.dial PSV @16:54:44.55 PS.seek 0ml @16:55:10.89 PSV.dial PBV @16:55:11.37 PS.seek 2.5ml @16:55:37.80 PSV.dial :waste1 @16:55:38.54 PS.seek 0ml @16:56:04.93 Garaging FlushPuck in Processing Clamp @16:56:05.00 Elbow.reconfigure :tight @16:56:05.04 Elbow.seek PC @16:56:05.25 Elbow.reconfigure :loose @16:56:05.35 Forearm.seek PC @16:56:06.78 Hand.close @16:56:07.42 PC.seek :open @16:56:12.13 Forearm.seek :retract @16:56:13.06 Elbow.reconfigure :tight @16:56:13.11 Elbow.seek :garage @16:56:14.48 Elbow.reconfigure :loose @16:56:14.57 Forearm.reconfigure :tight @16:56:14.61 Forearm.seek :garage @16:56:15.54 Hand.open @16:56:16.23 Forearm.reconfigure :loose @16:56:16.27 Forearm.seek :retract @16:59:26.93 CBV.dial :waste2b @16:59:27.25 CS.reconfigure :default @16:59:27.30 CS.seek 0ml @16:59:29.77 delay 1:00 @17:00:29.80 CTV.dial :air,avoiding: :intake @17:00:31.18 Storing wcr puck in Collection Clamp to tube 1 @17:00:31.56 Elbow.reconfigure :tight @17:00:31.60 Elbow.seek 1 @17:00:33.54 Forearm.reconfigure :tight @17:00:33.58 Forearm.seek 1 + 450 counts @17:00:34.62 SE.reconfigure :pick @17:00:34.67 SE.seek :beamBreak @17:00:36.85 SE.configure :default @17:00:36.99 Elbow.reconfigure :limp @17:00:37.04 Forearm.reconfigure :loose @17:00:37.09 Forearm.seek 1 @17:00:37.33 Elbow.hold @17:00:37.40 Elbow.reconfigure :tight @17:00:37.50 SE.seek 14.1 Pucks below Top Plate @17:00:37.97 Forearm.force -20 @17:00:38.01 Forearm.seek 1 + 292 counts @17:00:38.32 Forearm.reconfigure :tight @17:00:38.36 Forearm.seek 1 + 450 counts @17:00:38.58 SE.seek 15.1 Pucks below Top Plate @17:00:39.13 Forearm.reconfigure :loose @17:00:39.17 Forearm.seek :retract @17:00:40.09 Elbow.seek CC @17:00:41.77 Elbow.reconfigure :loose @17:00:41.86 Forearm.seek CC @17:00:42.87 Hand.close @17:00:43.42 CC.seek :open @17:00:48.15 Forearm.seek :retract @17:00:49.10 Elbow.reconfigure :tight @17:00:49.15 Elbow.seek 1 @17:00:50.96 Elbow.reconfigure :limp @17:00:51.01 Forearm.seek 1 @17:00:51.99 Elbow.hold @17:00:52.06 Elbow.reconfigure :tight @17:00:52.11 Hand.open @17:00:52.78 Forearm.force -20 @17:00:52.81 Forearm.seek 1 + 319 counts @17:00:53.13 Forearm.reconfigure :tight @17:00:53.18 Forearm.seek 1 + 450 counts @17:00:53.48 SE.seek :down @17:00:54.61 Forearm.reconfigure :loose @17:00:54.65 Forearm.seek :retract @17:00:55.14 SE.stop @17:00:56.08
Starting finalPurge @17:00:56.09 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @17:00:56.27 Elbow.seek FlushPuck::Garage @17:00:58.07 Elbow.reconfigure :loose @17:00:58.16 Forearm.seek :garage @17:00:59.15 Hand.close @17:00:59.91 Forearm.seek :retract @17:01:00.90 Elbow.reconfigure :tight @17:01:00.94 Elbow.seek CC @17:01:03.99 Elbow.reconfigure :loose @17:01:04.08 Forearm.reconfigure :tight @17:01:04.12 Forearm.seek CC @17:01:05.39 CC.seek :closed @17:01:09.92 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.25 @17:01:10.00 Hand.open @17:01:10.72 Forearm.reconfigure :loose @17:01:10.77 Forearm.seek :retract @17:01:11.71 Flushing the Collection Valve::Manifold @17:01:11.83 CSV.dial CSR @17:01:12.42 Pulling 5ml of CSR.flush @17:01:12.43 CSR.select :flush @17:01:12.55 CS.seek 5ml @17:01:26.15 delay 5 seconds @17:01:31.17 CSR.select :air @17:01:31.31 CS.reconfigure :fast @17:01:31.36 CS.seek 7.5ml @17:01:37.99 CSV.dial :waste @17:01:39.25 CS.reconfigure :default @17:01:39.29 CS.seek 0ml @17:01:59.27 Flushing lower manifold's waste1 port @17:01:59.39 CSV.dial :flush @17:01:59.84 CS.reconfigure :fast @17:01:59.88 CS.seek 5ml @17:02:12.68 delay 5 seconds @17:02:17.70 CSV.dial :air @17:02:18.19 CS.seek 7ml @17:02:23.78 CSV.dial CTV @17:02:23.79
CTV.dial CSV @17:02:25.16 CBV.dial :waste1 @17:02:25.90 CS.seek 0ml @17:02:42.46 Flushing lower manifold's waste2 port @17:02:42.58 CSV.dial :flush @17:02:43.31 CS.seek 5ml @17:02:55.83 delay 5 seconds @17:03:00.85 CSV.dial :air @17:03:01.36 CS.seek 7.001ml @17:03:06.77 CSV.dial CTV @17:03:06.79
CTV.dial CSV @17:03:07.24 CBV.dial :waste2 @17:03:08.28 CS.seek 0ml @17:03:24.74 Flushing lower manifold's waste2b port @17:03:24.86 CSV.dial :flush @17:03:25.70 CS.seek 5ml @17:03:38.18 delay 5 seconds @17:03:43.20 CSV.dial :air @17:03:43.69 CS.seek 7ml @17:03:49.21 CSV.dial CTV @17:03:49.23
CTV.dial CSV @17:03:49.68 CBV.dial :waste2b @17:03:50.72 CS.seek 0ml @17:04:07.15 Disinfecting external Intake with kill @17:04:07.26 CSV.dial CSR @17:04:07.75 Pulling 8ml of CSR.kill @17:04:07.76 CSR.select :kill @17:04:07.87 CS.seek 8ml @17:04:27.62 delay 5 seconds @17:04:32.63 CSR.select :air @17:04:32.74 CS.seek 10ml @17:04:38.18 CSV.dial CBV @17:04:38.20
CBV.dial CSV @17:04:39.25 CTV.dial :intake @17:04:40.02 Intake.select :open @17:04:40.13 CS.seek 2ml @17:04:58.64 Intake.select :close @17:04:58.74 CTV.dial :air @17:04:59.49 CS.reconfigure :default @17:04:59.53 CS.seek 0ml @17:05:05.46 CSV.dial CSR @17:05:06.50 Pulling 5ml of CSR.flush @17:05:06.97 CSR.select :flush @17:05:07.10 CS.seek 5ml @17:05:20.68 delay 5 seconds @17:05:25.70 CSR.select :air @17:05:25.81 CS.seek 10ml @17:05:39.36 CSV.dial CBV @17:05:40.37 CS.seek 0ml @17:06:06.77 Garaging FlushPuck in Collection Clamp @17:06:06.84 Elbow.reconfigure :tight @17:06:06.88 Elbow.seek CC @17:06:07.00 Elbow.reconfigure :loose @17:06:07.09 Forearm.seek CC @17:06:08.05 Hand.close @17:06:08.66 CC.seek :open @17:06:13.32 Forearm.seek :retract @17:06:14.27 Elbow.reconfigure :tight @17:06:14.32 Elbow.seek :garage @17:06:17.26 Elbow.reconfigure :loose @17:06:17.36 Forearm.reconfigure :tight @17:06:17.40 Forearm.seek :garage @17:06:18.41 Hand.open @17:06:19.09 Forearm.reconfigure :loose @17:06:19.14 Forearm.seek :retract @17:06:20.11 Completed [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] begun 14:31:55 @17:06:20.16 Asleep after completing DA_WCR_112222 assays @17:06:20.18 GoodNews.email "{1 => 16,\n 2 => 17,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@17:01:04, 16% flow, 23.8C, 22% humidity, 15.0psia, 13.778V, 0.363A, 0.351A avg, 5.00W\nSampled 700.0ml",Subject: "Asleep after completing DA_WCR_112222 assays" @17:06:26.30 upsync @15:23:43.48UTC29-Nov-22
ESP2 v3.41 I2C Gateway v3.20 for core bus @15:23:46.05 Configuration for short puck jake 1/11/21 by brent@mbari.org /home/esp/esp2/type/shallow/jake/configure.rb @15:23:50.01 Polling Can every 10:00 @15:23:50.04 Cleanly exited at 15:23:21UTC29-Nov-22 @15:23:50.08 Loading /home/esp/esp2/mission/DA_WCR_112922.rb mission @15:23:50.13 Preparing to sleep until + 00:00:05 @15:23:50.14 GoodNews.email "Starting DA_WCR_112922 w/pucks in tube 2\n{1 => 16,\n 2 => 17,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@15:23:50, 16% flow, 19.1C, 26% humidity, 14.8psia, 13.783V, 0.327A, 0.309A avg, 4.50W",Subject: "DA_WCR_112922 begun" @15:23:50.20
Asleep til + 00:00:05 @15:23:50.22 Continuing at 15:23 @15:23:50.48 Gate.power :core,:OFF @15:23:50.50 Gate.power :raw,:OFF @15:23:50.51 Shutdown @15:23:55.12
Powering core bus @15:23:55.30 GoodNews.email "Then, in tube 2: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2/mission/DA_WCR_112922.rb:14\n{1 => 16,\n 2 => 17,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@15:23:50, 16% flow, 19.1C, 26% humidity, 14.8psia, 13.783V, 0.327A, 0.309A avg, 4.50W",Subject: "Asleep until 15:23" @15:23:57.33 upsync @15:24:00.42
Gate.power :raw,:ON @15:24:00.77 upsync @15:24:01.31
CS.configure :default @15:24:01.40 PS.configure :default @15:24:01.49 SS.configure :default @15:24:01.58 CC.configure :default @15:24:01.66 PC.configure :default @15:24:01.77 Forearm.configure :loose @15:24:01.85 Elbow.configure :loose @15:24:01.96 SC.configure :loose @15:24:02.04 SE.configure :default @15:24:02.08 CH.coast @15:24:02.13 CH.configure :default @15:24:02.16 PH.coast @15:24:02.21 PH.configure :default @15:24:02.25 All dwarf controllers running firmware version 3.85 @15:24:02.35 Valve.reset @15:24:02.53 Intake.select :close @15:24:02.58 Exhaust.select :close @15:24:02.63 CTV.dial :air @15:24:02.73 PSV.dial :air @15:24:02.84 CSV.dial :waste1 @15:24:02.86
Forearm.reconfigure :loose @15:24:02.94 Forearm.home.seek :retract @15:24:03.32 SSV.dial :spit,avoiding: :exhaust @15:24:03.64 Hand.close @15:24:03.68
Elbow.jog 1500 @15:24:03.79 PS.reconfigure :default @15:24:03.84 PS.home.seek 0ml @15:24:04.13 CS.reconfigure :fast @15:24:04.15 SS.reconfigure :default @15:24:04.24 CS.home.seek 0ml @15:24:04.27 SS.home.seek 0ml @15:24:04.39
Elbow.reconfigure :loose @15:24:04.43 Elbow.home.seek :clear @15:24:08.88 SE.reconfigure :default @15:24:08.92 SE.home.seek :down @15:24:09.52 SC.reconfigure :tight @15:24:09.57 SC.home.seek :initial @15:24:12.15 PS.stop @15:24:37.85
SC.configure :limp @15:24:37.90 Forearm.seek :retract @15:24:37.95 Elbow.seek :hammer @15:24:38.98 Forearm.reconfigure :tight @15:24:39.02 Forearm.seek retracted @15:24:39.25 PC.reconfigure :default @15:24:39.27
CC.reconfigure :default @15:24:39.35 PC.home.seek :open @15:24:39.38
CC.home.seek :open @15:24:42.89 Phase [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] @15:24:42.90 Notice.email "At 15:24:42 began in tube 2: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2/mission/DA_WCR_112922.rb:14\n{1 => 16,\n 2 => 17,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@15:24:42, 16% flow, 19.2C, 26% humidity, 14.8psia, 13.788V, 0.327A, 0.302A avg, 4.50W",Subject: "Started DA_WCR_112922 phase initialPurge; da [1000, 1000] #da and wsr are run simultanelously" @15:24:42.92
Starting initialPurge @15:24:42.95 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @15:24:43.01 Hand.open @15:24:43.68 Elbow.reconfigure :tight @15:24:43.74 Elbow.seek FlushPuck::Garage @15:24:45.61 Elbow.reconfigure :loose @15:24:45.70 Forearm.reconfigure :loose @15:24:45.74 Forearm.seek :garage @15:24:46.79 Hand.close @15:24:47.56 Forearm.seek :retract @15:24:48.49 upsync @15:24:48.56
Elbow.reconfigure :tight @15:24:48.60 Elbow.seek CC @15:24:51.52 Elbow.reconfigure :loose @15:24:51.61 Forearm.reconfigure :tight @15:24:51.65 Forearm.seek CC @15:24:52.94 CC.seek :closed @15:24:57.48 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.24 @15:24:57.56 Hand.open @15:24:58.24 Forearm.reconfigure :loose @15:24:58.28 Forearm.seek :retract @15:24:59.31 Flushing the Collection Valve::Manifold @15:24:59.43 CSV.dial CSR @15:25:00.76 Pulling 5ml of CSR.flush @15:25:00.77 CSR.select :flush @15:25:00.88 CS.reconfigure :default @15:25:00.92 CS.seek 5ml @15:25:14.51 delay 5 seconds @15:25:19.53 CSR.select :air @15:25:19.67 CS.reconfigure :fast @15:25:19.71 CS.seek 7.5ml @15:25:26.44 CSV.dial :waste @15:25:27.70 CS.reconfigure :default @15:25:27.74 CS.seek 0ml @15:25:47.79 Purging external Intake @15:25:47.90 CSV.dial :flush @15:25:48.46 CS.reconfigure :fast @15:25:48.51 CS.seek 10ml @15:26:12.79 delay 5 seconds @15:26:17.81 CSV.dial CBV @15:26:17.84
CBV.dial CSV @15:26:18.57 CTV.dial :intake @15:26:19.35 Intake.select :open @15:26:19.45 CS.seek 0ml @15:26:42.84 Intake.select :close @15:26:42.94 CTV.dial :air @15:26:43.74 Flushing upper manifold @15:26:43.90 CSV.dial :flush @15:26:44.69 CS.seek 5ml @15:26:57.47 delay 5 seconds @15:27:02.49 CSV.dial :air @15:27:02.99 CS.seek 7ml @15:27:08.45 CSV.dial CBV @15:27:08.47
CBV.dial CSV @15:27:09.49 CTV.dial :air @15:27:09.51 CS.seek 0ml @15:27:26.07 delay 7 seconds @15:27:33.09 CS.seek 3ml @15:27:41.03 CSV.dial :waste @15:27:41.48 CS.seek 0ml @15:27:49.17 CSV.dial :flush @15:27:49.60 CS.seek 3ml @15:27:57.53 delay 5 seconds @15:28:02.55 CSV.dial :air @15:28:03.05 CS.seek 5ml @15:28:08.69 CSV.dial :air @15:28:08.71 CS.seek 0ml @15:28:20.78 delay 7 seconds @15:28:27.81 CS.seek 3ml @15:28:35.78 CSV.dial :waste @15:28:36.51 CS.seek 0ml @15:28:44.16 Garaging FlushPuck in Collection Clamp @15:28:44.23 Elbow.reconfigure :tight @15:28:44.27 Elbow.seek CC @15:28:44.53 Elbow.reconfigure :loose @15:28:44.62 Forearm.seek CC @15:28:45.60 Hand.close @15:28:46.18 CC.seek :open @15:28:50.83 Forearm.seek :retract @15:28:51.79 Elbow.reconfigure :tight @15:28:51.83 Elbow.seek :garage @15:28:54.84 Elbow.reconfigure :loose @15:28:54.93 Forearm.reconfigure :tight @15:28:54.97 Forearm.seek :garage @15:28:56.03 Hand.open @15:28:57.11 Forearm.reconfigure :loose @15:28:57.15 Forearm.seek :retract @15:28:58.12 da sampling at most 1000ml, wcr at most 1000ml @15:28:58.15 DAfans.email "Started da in tube 2 at 15:28:58UTC29-Nov-22\n{1 => 16,\n 2 => 17,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@15:24:42, 16% flow, 19.2C, 26% humidity, 14.8psia, 13.788V, 0.327A, 0.302A avg, 4.50W",Subject: "da sampling at most 1000ml, wcr at most 1000ml" @15:28:58.17
Loading sh1 puck from tube 2 into Collection Clamp @15:28:58.41 SC.reconfigure :tight @15:28:58.45 SC.seek 2 @15:29:04.13 upsync @15:29:05.13
SC.configure :limp @15:29:05.34 Elbow.reconfigure :tight @15:29:05.38 Elbow.seek 2 @15:29:07.31 Forearm.reconfigure :tight @15:29:07.36 Forearm.seek 2 + 450 counts @15:29:08.40 SE.reconfigure :pick @15:29:08.45 SE.seek :beamBreak @15:29:10.63 SE.configure :default @15:29:10.77 Elbow.seek 2 @15:29:10.94 Forearm.seek 2 + 300 counts @15:29:11.19 Forearm.reconfigure :loose @15:29:11.23 Hand.close @15:29:11.82 SE.seek :down @15:29:12.02
Forearm.seek :retract @15:29:13.01 Elbow.seek CC @15:29:13.54 SE.stop @15:29:14.64
Elbow.reconfigure :loose @15:29:14.73 Forearm.reconfigure :tight @15:29:14.78 Forearm.seek CC @15:29:16.02 CC.seek :closed @15:29:20.53 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.18 @15:29:20.54 Hand.open @15:29:21.24 Forearm.reconfigure :loose @15:29:21.29 Forearm.seek :retract @15:29:22.23 collectSample 1000ml w/bubble point of 24psi @15:29:22.24 Priming 7ml @15:29:22.25 Intake.select :close @15:29:22.26 Exhaust.select :close @15:29:22.27 SS.hold @15:29:22.46 Calibrating pressure and temperature sensors @15:29:22.49 CTV.dial :air @15:29:22.50 SSV.dial :spit,avoiding: :exhaust @15:29:22.52 SS.reconfigure :fast @15:29:22.57 SS.seek 5ml @15:29:25.36 SSV.dial CBV @15:29:25.38
CBV.dial SSV @15:29:26.70 delay 3.8 seconds @15:29:30.61 SSV.dial :spit @15:29:31.91 SS.seek 0ml @15:29:34.87 SSV.dial CBV @15:29:36.17 CTV.dial :intake @15:29:36.95 SSV.dial CBV @15:29:36.96
CBV.dial SSV @15:29:36.98 Allowing 40s for 7.0ml of remaining 7.0ml @15:29:36.99 Intake.select :open @15:29:39.15 Intake @15.5psia, vacuum from 10.0 to 13.0psi @15:29:39.17 SS.reconfigure :inhaling 2.20ml/s @15:29:39.21 SS.seek 7ml @15:29:44.92 Stroke completed -- waiting for pressure to equalize ... @15:29:44.93 SS.reconfigure :exhale @15:29:44.97 SS.hold @15:29:46.15 Intake.select :close @15:29:46.29 SSV.dial :exhaust @15:29:47.03 Exhaust.select :open @15:29:47.14 SS.seek 0ml @15:29:51.14 Exhaust.select :close @15:29:51.24 Sampling 1000.0ml @15:29:51.25 Intake.select :close @15:29:51.26 Exhaust.select :close @15:29:51.27 SS.reconfigure :default @15:29:51.31 SS.hold @15:29:51.49 CTV.dial :intake @15:29:51.50 SSV.dial CBV @15:29:51.52
CBV.dial SSV @15:29:52.28 Allowing 130s for 25.0ml of remaining 1000.0ml @15:29:52.29 Intake.select :open @15:29:54.44 Intake @15.6psia, vacuum from 10.0 to 13.0psi @15:29:54.46 SS.reconfigure :inhaling 2.20ml/s @15:29:54.50 SS.seek 25ml @15:30:09.40 Stroke completed -- waiting for pressure to equalize ... @15:30:09.41 SS.reconfigure :exhale @15:30:09.45 SS.hold @15:30:13.65 Intake.select :close @15:30:13.79 SSV.dial :exhaust @15:30:14.53 Exhaust.select :open @15:30:14.64 SS.seek 0ml @15:30:23.34 Exhaust.select :close @15:30:23.44 SSV.dial CBV @15:30:23.46
CBV.dial SSV @15:30:24.22 Allowing 130s for 25.0ml of remaining 975.0ml @15:30:24.23 Intake.select :open @15:30:26.38 Intake @15.5psia, vacuum from 10.0 to 13.0psi @15:30:26.39 SS.reconfigure :inhaling 2.20ml/s @15:30:26.44 SS.seek 25ml @15:30:42.06 Stroke completed -- waiting for pressure to equalize ... @15:30:42.07 SS.reconfigure :exhale @15:30:42.11 SS.hold @15:30:46.87 Intake.select :close @15:30:47.01 SSV.dial :exhaust @15:30:47.75 Exhaust.select :open @15:30:47.85 SS.seek 0ml @15:30:56.62 Exhaust.select :close @15:30:56.72 SSV.dial CBV @15:30:56.74
CBV.dial SSV @15:30:57.50 Allowing 130s for 25.0ml of remaining 950.0ml @15:30:57.51 Intake.select :open @15:30:59.66 Intake @15.6psia, vacuum from 10.0 to 13.0psi @15:30:59.68 SS.reconfigure :inhaling 2.20ml/s @15:30:59.72 SS.seek 25ml @15:31:14.73 Stroke completed -- waiting for pressure to equalize ... @15:31:14.74 SS.reconfigure :exhale @15:31:14.78 SS.hold @15:31:20.30 Intake.select :close @15:31:20.44 SSV.dial :exhaust @15:31:21.18 Exhaust.select :open @15:31:21.29 SS.seek 0ml @15:31:30.20 Exhaust.select :close @15:31:30.30 SSV.dial CBV @15:31:30.32
CBV.dial SSV @15:31:31.07 Allowing 130s for 25.0ml of remaining 925.0ml @15:31:31.08 Intake.select :open @15:31:33.24 Intake @15.6psia, vacuum from 10.0 to 13.0psi @15:31:33.25 SS.reconfigure :inhaling 2.20ml/s @15:31:33.29 SS.seek 25ml @15:31:47.93 Stroke completed -- waiting for pressure to equalize ... @15:31:47.94 SS.reconfigure :exhale @15:31:47.98 SS.hold @15:31:53.51 Intake.select :close @15:31:53.65 SSV.dial :exhaust @15:31:54.38 Exhaust.select :open @15:31:54.49 SS.seek 0ml @15:32:03.13 Exhaust.select :close @15:32:03.24 SSV.dial CBV @15:32:03.28
CBV.dial SSV @15:32:04.01 Allowing 130s for 25.0ml of remaining 900.0ml @15:32:04.02 Intake.select :open @15:32:06.17 Intake @15.5psia, vacuum from 10.0 to 13.0psi @15:32:06.19 SS.reconfigure :inhaling 2.20ml/s @15:32:06.23 SS.seek 25ml @15:32:21.34 Stroke completed -- waiting for pressure to equalize ... @15:32:21.35 SS.reconfigure :exhale @15:32:21.39 SS.hold @15:32:26.93 Intake.select :close @15:32:27.06 SSV.dial :exhaust @15:32:27.82 Exhaust.select :open @15:32:27.93 SS.seek 0ml @15:32:36.93 Exhaust.select :close @15:32:37.03 SSV.dial CBV @15:32:37.05
CBV.dial SSV @15:32:37.82 Allowing 130s for 25.0ml of remaining 875.0ml @15:32:37.83 Intake.select :open @15:32:39.98 Intake @15.5psia, vacuum from 10.0 to 13.0psi @15:32:40.00 SS.reconfigure :inhaling 2.20ml/s @15:32:40.04 SS.seek 25ml @15:32:54.49 Stroke completed -- waiting for pressure to equalize ... @15:32:54.50 SS.reconfigure :exhale @15:32:54.54 SS.hold @15:33:00.95 Intake.select :close @15:33:01.09 SSV.dial :exhaust @15:33:01.95 Exhaust.select :open @15:33:02.05 SS.seek 0ml @15:33:10.65 Exhaust.select :close @15:33:10.75 SSV.dial CBV @15:33:10.77
CBV.dial SSV @15:33:11.54 Allowing 130s for 25.0ml of remaining 850.0ml @15:33:11.55 Intake.select :open @15:33:13.70 Intake @15.6psia, vacuum from 10.0 to 13.0psi @15:33:13.72 SS.reconfigure :inhaling 2.20ml/s @15:33:13.76 SS.seek 25ml @15:33:28.80 Stroke completed -- waiting for pressure to equalize ... @15:33:28.81 SS.reconfigure :exhale @15:33:28.85 SS.hold @15:33:35.25 Intake.select :close @15:33:35.38 SSV.dial :exhaust @15:33:36.12 Exhaust.select :open @15:33:36.22 SS.seek 0ml @15:33:44.96 Exhaust.select :close @15:33:45.07 SSV.dial CBV @15:33:45.08
CBV.dial SSV @15:33:45.84 Allowing 130s for 25.0ml of remaining 825.0ml @15:33:45.85 Intake.select :open @15:33:48.00 Intake @15.6psia, vacuum from 10.0 to 13.0psi @15:33:48.02 SS.reconfigure :inhaling 2.20ml/s @15:33:48.06 SS.seek 25ml @15:34:02.85 Stroke completed -- waiting for pressure to equalize ... @15:34:02.86 SS.reconfigure :exhale @15:34:02.90 SS.hold @15:34:10.25 Intake.select :close @15:34:10.39 SSV.dial :exhaust @15:34:11.13 Exhaust.select :open @15:34:11.24 SS.seek 0ml @15:34:20.27 Exhaust.select :close @15:34:20.37 SSV.dial CBV @15:34:20.40
CBV.dial SSV @15:34:21.15 Allowing 130s for 25.0ml of remaining 800.0ml @15:34:21.16 Intake.select :open @15:34:23.31 Intake @15.6psia, vacuum from 10.0 to 13.0psi @15:34:23.33 SS.reconfigure :inhaling 2.20ml/s @15:34:23.37 SS.seek 25ml @15:34:36.84 Vacuum too high -- waiting up to 40 seconds @15:34:36.85 SS.reconfigure :exhale @15:34:36.89 SS.hold @15:34:37.56 Intake @11.7psia, vacuum from 6.2 to 9.2psi @15:34:37.58 SS.reconfigure :inhaling 1.98ml/s @15:34:37.62 SS.seek 25ml @15:34:39.40 Stroke completed -- waiting for pressure to equalize ... SS.reconfigure :exhale @15:34:39.44 SS.hold @15:34:45.91 Intake.select :close @15:34:46.05 SSV.dial :exhaust @15:34:46.79 Exhaust.select :open @15:34:46.89 SS.seek 0ml @15:34:55.69 Exhaust.select :close @15:34:55.79 SSV.dial CBV @15:34:55.81
CBV.dial SSV @15:34:56.57 Allowing 130s for 25.0ml of remaining 775.0ml @15:34:56.58 Intake.select :open @15:34:58.73 Intake @15.5psia, vacuum from 10.0 to 13.0psi @15:34:58.75 SS.reconfigure :inhaling 1.98ml/s @15:34:58.79 SS.seek 25ml @15:35:14.99 Stroke completed -- waiting for pressure to equalize ... @15:35:15.00 SS.reconfigure :exhale @15:35:15.04 SS.hold @15:35:22.42 Intake.select :close @15:35:22.55 SSV.dial :exhaust @15:35:23.30 Exhaust.select :open @15:35:23.41 SS.seek 0ml @15:35:32.10 Exhaust.select :close @15:35:32.20 SSV.dial CBV @15:35:32.22
CBV.dial SSV @15:35:32.98 Allowing 130s for 25.0ml of remaining 750.0ml @15:35:32.99 Intake.select :open @15:35:35.14 Intake @15.6psia, vacuum from 10.0 to 13.0psi @15:35:35.15 SS.reconfigure :inhaling 1.98ml/s @15:35:35.20 SS.seek 25ml @15:35:51.33 Stroke completed -- waiting for pressure to equalize ... @15:35:51.34 SS.reconfigure :exhale @15:35:51.38 SS.hold @15:35:58.88 Intake.select :close @15:35:59.02 SSV.dial :exhaust @15:35:59.76 Exhaust.select :open @15:35:59.86 SS.seek 0ml @15:36:08.64 Exhaust.select :close @15:36:08.75 SSV.dial CBV @15:36:08.77
CBV.dial SSV @15:36:09.54 Allowing 130s for 25.0ml of remaining 725.0ml @15:36:09.55 Intake.select :open @15:36:11.70 Intake @15.5psia, vacuum from 10.0 to 13.0psi @15:36:11.72 SS.reconfigure :inhaling 1.98ml/s @15:36:11.76 SS.seek 25ml @15:36:27.58 Stroke completed -- waiting for pressure to equalize ... @15:36:27.59 SS.reconfigure :exhale @15:36:27.63 SS.hold @15:36:36.08 Intake.select :close @15:36:36.22 SSV.dial :exhaust @15:36:36.96 Exhaust.select :open @15:36:37.06 SS.seek 0ml @15:36:45.67 Exhaust.select :close @15:36:45.78 SSV.dial CBV @15:36:45.86
CBV.dial SSV @15:36:46.58 Allowing 130s for 25.0ml of remaining 700.0ml @15:36:46.59 Intake.select :open @15:36:48.74 Intake @15.6psia, vacuum from 10.0 to 13.0psi @15:36:48.76 SS.reconfigure :inhaling 1.98ml/s @15:36:48.80 SS.seek 25ml @15:37:01.93 Vacuum too high -- waiting up to 40 seconds @15:37:01.94 SS.reconfigure :exhale @15:37:01.98 SS.hold @15:37:02.59 Intake @12.1psia, vacuum from 6.6 to 9.6psi @15:37:02.60 SS.reconfigure :inhaling 1.79ml/s @15:37:02.65 SS.seek 25ml @15:37:06.47 Stroke completed -- waiting for pressure to equalize ... @15:37:06.48 SS.reconfigure :exhale @15:37:06.52 SS.hold @15:37:13.89 Intake.select :close @15:37:14.02 SSV.dial :exhaust @15:37:14.77 Exhaust.select :open @15:37:14.88 SS.seek 0ml @15:37:23.83 Exhaust.select :close @15:37:23.93 SSV.dial CBV @15:37:23.95
CBV.dial SSV @15:37:24.71 Allowing 130s for 25.0ml of remaining 675.0ml @15:37:24.72 Intake.select :open @15:37:26.87 Intake @15.5psia, vacuum from 10.0 to 13.0psi @15:37:26.89 SS.reconfigure :inhaling 1.79ml/s @15:37:26.93 SS.seek 25ml @15:37:43.06 Vacuum too high -- waiting up to 40 seconds @15:37:43.07 SS.reconfigure :exhale @15:37:43.11 SS.hold @15:37:43.70 Intake @12.2psia, vacuum from 6.7 to 9.7psi @15:37:43.72 SS.reconfigure :inhaling 1.61ml/s @15:37:43.76 SS.seek 25ml @15:37:44.33 Stroke completed -- waiting for pressure to equalize ... @15:37:44.34 SS.reconfigure :exhale @15:37:44.38 SS.hold @15:37:51.84 Intake.select :close @15:37:51.98 SSV.dial :exhaust @15:37:52.72 Exhaust.select :open @15:37:52.83 SS.seek 0ml @15:38:01.58 Exhaust.select :close @15:38:01.68 SSV.dial CBV @15:38:01.70
CBV.dial SSV @15:38:02.46 Allowing 130s for 25.0ml of remaining 650.0ml @15:38:02.47 Intake.select :open @15:38:04.62 Intake @15.5psia, vacuum from 10.0 to 13.0psi @15:38:04.63 SS.reconfigure :inhaling 1.61ml/s @15:38:04.68 SS.seek 25ml @15:38:23.12 Stroke completed -- waiting for pressure to equalize ... @15:38:23.13 SS.reconfigure :exhale @15:38:23.18 SS.hold @15:38:31.62 Intake.select :close @15:38:31.76 SSV.dial :exhaust @15:38:32.50 Exhaust.select :open @15:38:32.61 SS.seek 0ml @15:38:41.50 Exhaust.select :close @15:38:41.61 SSV.dial CBV @15:38:41.66
CBV.dial SSV @15:38:42.36 Allowing 130s for 25.0ml of remaining 625.0ml @15:38:42.37 Intake.select :open @15:38:44.52 Intake @15.5psia, vacuum from 10.0 to 13.0psi @15:38:44.54 SS.reconfigure :inhaling 1.61ml/s @15:38:44.58 SS.seek 25ml @15:39:01.49 Vacuum too high -- waiting up to 40 seconds @15:39:01.50 SS.reconfigure :exhale @15:39:01.54 SS.hold @15:39:02.08 Intake @12.5psia, vacuum from 7.0 to 10.0psi @15:39:02.10 SS.reconfigure :inhaling 1.45ml/s @15:39:02.14 SS.seek 25ml @15:39:04.81 Stroke completed -- waiting for pressure to equalize ... @15:39:04.82 SS.reconfigure :exhale @15:39:04.86 SS.hold @15:39:12.33 Intake.select :close @15:39:12.47 SSV.dial :exhaust @15:39:13.20 Exhaust.select :open @15:39:13.31 SS.seek 0ml @15:39:22.33 Exhaust.select :close @15:39:22.43 SSV.dial CBV @15:39:22.45
CBV.dial SSV @15:39:23.21 Allowing 130s for 25.0ml of remaining 600.0ml Intake.select :open @15:39:25.37 Intake @15.6psia, vacuum from 10.0 to 13.0psi @15:39:25.38 SS.reconfigure :inhaling 1.45ml/s @15:39:25.42 SS.seek 25ml @15:39:43.96 Vacuum too high -- waiting up to 40 seconds @15:39:43.97 SS.reconfigure :exhale @15:39:44.01 SS.hold @15:39:45.36 Intake @12.9psia, vacuum from 7.4 to 10.4psi @15:39:45.37 SS.reconfigure :inhaling 1.30ml/s @15:39:45.42 SS.seek 25ml @15:39:47.19 Stroke completed -- waiting for pressure to equalize ... @15:39:47.20 SS.reconfigure :exhale @15:39:47.24 SS.hold @15:39:54.60 Intake.select :close @15:39:54.74 SSV.dial :exhaust @15:39:55.47 Exhaust.select :open @15:39:55.58 SS.seek 0ml @15:40:04.33 Exhaust.select :close @15:40:04.43 SSV.dial CBV @15:40:04.45
CBV.dial SSV @15:40:05.20 Allowing 130s for 25.0ml of remaining 575.0ml @15:40:05.21 Intake.select :open @15:40:07.37 Intake @15.5psia, vacuum from 10.0 to 13.0psi @15:40:07.39 SS.reconfigure :inhaling 1.30ml/s @15:40:07.43 SS.seek 25ml @15:40:29.54 Stroke completed -- waiting for pressure to equalize ... @15:40:29.55 SS.reconfigure :exhale @15:40:29.59 SS.hold @15:40:38.04 Intake.select :close @15:40:38.18 SSV.dial :exhaust @15:40:38.92 Exhaust.select :open @15:40:39.03 SS.seek 0ml @15:40:47.83 Exhaust.select :close @15:40:47.93 SSV.dial CBV @15:40:47.95
CBV.dial SSV @15:40:48.70 Allowing 130s for 25.0ml of remaining 550.0ml @15:40:48.71 Intake.select :open @15:40:50.86 Intake @15.6psia, vacuum from 10.0 to 13.0psi @15:40:50.88 SS.reconfigure :inhaling 1.30ml/s @15:40:50.92 SS.seek 25ml @15:41:08.78 Vacuum too high -- waiting up to 40 seconds @15:41:08.79 SS.reconfigure :exhale @15:41:08.83 SS.hold @15:41:11.87 Intake @13.6psia, vacuum from 8.1 to 11.1psi @15:41:11.88 SS.reconfigure :inhaling 1.17ml/s @15:41:11.93 SS.seek 25ml @15:41:17.25 Stroke completed -- waiting for pressure to equalize ... @15:41:17.26 SS.reconfigure :exhale @15:41:17.30 SS.hold @15:41:23.32 Intake.select :close @15:41:23.45 SSV.dial :exhaust @15:41:24.20 Exhaust.select :open @15:41:24.31 SS.seek 0ml @15:41:33.31 Exhaust.select :close @15:41:33.41 SSV.dial CBV @15:41:33.43
CBV.dial SSV @15:41:34.19 Allowing 130s for 25.0ml of remaining 525.0ml @15:41:34.20 Intake.select :open @15:41:36.35 Intake @15.2psia, vacuum from 9.7 to 12.7psi @15:41:36.37 SS.reconfigure :inhaling 1.17ml/s @15:41:36.41 SS.seek 25ml @15:41:59.98 Stroke completed -- waiting for pressure to equalize ... @15:41:59.99 SS.reconfigure :exhale @15:42:00.03 SS.hold @15:42:09.69 Intake.select :close @15:42:09.82 SSV.dial :exhaust @15:42:10.56 Exhaust.select :open @15:42:10.67 SS.seek 0ml @15:42:19.46 Exhaust.select :close @15:42:19.57 SSV.dial CBV @15:42:19.59
CBV.dial SSV @15:42:20.34 Allowing 130s for 25.0ml of remaining 500.0ml @15:42:20.35 Intake.select :open @15:42:22.50 Intake @15.7psia, vacuum from 10.0 to 13.0psi @15:42:22.52 SS.reconfigure :inhaling 1.17ml/s @15:42:22.56 SS.seek 25ml @15:42:39.46 Vacuum too high -- waiting up to 40 seconds @15:42:39.47 SS.reconfigure :exhale @15:42:39.51 SS.hold @15:42:43.04 Intake @14.0psia, vacuum from 8.5 to 11.5psi @15:42:43.06 SS.reconfigure :inhaling 1.06ml/s @15:42:43.11 SS.seek 25ml @15:42:51.67 Stroke completed -- waiting for pressure to equalize ... @15:42:51.68 SS.reconfigure :exhale @15:42:51.72 SS.hold @15:43:00.17 Intake.select :close @15:43:00.31 SSV.dial :exhaust @15:43:01.06 Exhaust.select :open @15:43:01.16 SS.seek 0ml @15:43:10.15 Exhaust.select :close @15:43:10.25 SSV.dial CBV @15:43:10.28
CBV.dial SSV @15:43:11.03 Allowing 130s for 25.0ml of remaining 475.0ml @15:43:11.04 Intake.select :open @15:43:13.19 Intake @15.7psia, vacuum from 10.0 to 13.0psi @15:43:13.21 SS.reconfigure :inhaling 1.06ml/s @15:43:13.25 SS.seek 25ml @15:43:32.11 Vacuum too high -- waiting up to 40 seconds @15:43:32.12 SS.reconfigure :exhale @15:43:32.17 SS.hold @15:43:35.71 Intake @14.0psia, vacuum from 8.5 to 11.5psi @15:43:35.72 SS.reconfigure :inhaling 0.95ml/s @15:43:35.76 SS.seek 25ml @15:43:44.13 Stroke completed -- waiting for pressure to equalize ... @15:43:44.14 SS.reconfigure :exhale @15:43:44.18 SS.hold @15:43:52.90 Intake.select :close @15:43:53.04 SSV.dial :exhaust @15:43:53.78 Exhaust.select :open @15:43:53.88 SS.seek 0ml @15:44:02.93 Exhaust.select :close @15:44:03.03 SSV.dial CBV @15:44:03.06
CBV.dial SSV @15:44:03.81 Allowing 130s for 25.0ml of remaining 450.0ml @15:44:03.82 Intake.select :open @15:44:05.97 Intake @15.5psia, vacuum from 10.0 to 13.0psi @15:44:05.99 SS.reconfigure :inhaling 0.95ml/s @15:44:06.03 SS.seek 25ml @15:44:31.45 Vacuum too high -- waiting up to 40 seconds @15:44:31.46 SS.reconfigure :exhale @15:44:31.50 SS.hold @15:44:37.13 Intake @14.3psia, vacuum from 8.8 to 11.8psi @15:44:37.15 SS.reconfigure :inhaling 0.87ml/s @15:44:37.19 SS.seek 25ml @15:44:40.84 Stroke completed -- waiting for pressure to equalize ... @15:44:40.85 SS.reconfigure :exhale @15:44:40.89 SS.hold @15:44:48.28 Intake.select :close @15:44:48.41 SSV.dial :exhaust @15:44:49.15 Exhaust.select :open @15:44:49.26 SS.seek 0ml @15:44:58.29 Exhaust.select :close @15:44:58.39 SSV.dial CBV @15:44:58.41
CBV.dial SSV @15:44:59.17 Allowing 130s for 25.0ml of remaining 425.0ml @15:44:59.18 Intake.select :open @15:45:01.33 Intake @15.7psia, vacuum from 10.0 to 13.0psi @15:45:01.35 SS.reconfigure :inhaling 0.87ml/s @15:45:01.39 SS.seek 25ml @15:45:24.39 Vacuum too high -- waiting up to 40 seconds @15:45:24.40 SS.reconfigure :exhale @15:45:24.44 SS.hold @15:45:29.30 Intake @14.4psia, vacuum from 8.9 to 11.9psi @15:45:29.31 SS.reconfigure :inhaling 0.78ml/s @15:45:29.36 SS.seek 25ml @15:45:38.69 Stroke completed -- waiting for pressure to equalize ... @15:45:38.70 SS.reconfigure :exhale @15:45:38.74 SS.hold @15:45:48.41 Intake.select :close @15:45:48.55 SSV.dial :exhaust @15:45:49.29 Exhaust.select :open @15:45:49.40 SS.seek 0ml @15:45:58.18 Exhaust.select :close @15:45:58.28 SSV.dial CBV @15:45:58.30
CBV.dial SSV @15:45:59.06 Allowing 130s for 25.0ml of remaining 400.0ml @15:45:59.07 Intake.select :open @15:46:01.23 Intake @15.5psia, vacuum from 10.0 to 13.0psi @15:46:01.24 SS.reconfigure :inhaling 0.78ml/s @15:46:01.29 SS.seek 25ml @15:46:32.36 Vacuum too high -- waiting up to 40 seconds @15:46:32.37 SS.reconfigure :exhale @15:46:32.41 SS.hold @15:46:38.93 Intake @14.5psia, vacuum from 9.0 to 12.0psi @15:46:38.94 SS.reconfigure :inhaling 0.70ml/s @15:46:38.99 SS.seek 25ml @15:46:44.18 Stroke completed -- waiting for pressure to equalize ... @15:46:44.19 SS.reconfigure :exhale @15:46:44.23 SS.hold @15:46:52.66 Intake.select :close @15:46:52.80 SSV.dial :exhaust @15:46:53.54 Exhaust.select :open @15:46:53.65 SS.seek 0ml @15:47:02.60 Exhaust.select :close @15:47:02.70 SSV.dial CBV @15:47:02.72
CBV.dial SSV @15:47:03.48 Allowing 130s for 25.0ml of remaining 375.0ml @15:47:03.49 Intake.select :open @15:47:05.64 Intake @15.5psia, vacuum from 10.0 to 13.0psi @15:47:05.66 SS.reconfigure :inhaling 0.70ml/s @15:47:05.70 SS.seek 25ml @15:47:39.57 Vacuum too high -- waiting up to 40 seconds @15:47:39.58 SS.reconfigure :exhale @15:47:39.63 SS.hold @15:47:47.08 Intake @14.8psia, vacuum from 9.3 to 12.3psi @15:47:47.10 SS.reconfigure :inhaling 0.63ml/s @15:47:47.14 SS.seek 25ml @15:47:51.63 Stroke completed -- waiting for pressure to equalize ... @15:47:51.64 SS.reconfigure :exhale @15:47:51.68 SS.hold @15:48:01.38 Intake.select :close @15:48:01.52 SSV.dial :exhaust @15:48:02.26 Exhaust.select :open @15:48:02.37 SS.seek 0ml @15:48:11.41 Exhaust.select :close @15:48:11.51 SSV.dial CBV @15:48:11.53
CBV.dial SSV @15:48:12.29 Allowing 130s for 25.0ml of remaining 350.0ml @15:48:12.30 Intake.select :open @15:48:14.45 Intake @15.6psia, vacuum from 10.0 to 13.0psi @15:48:14.47 SS.reconfigure :inhaling 0.63ml/s @15:48:14.51 SS.seek 25ml @15:48:44.01 Vacuum too high -- waiting up to 40 seconds @15:48:44.02 SS.reconfigure :exhale @15:48:44.06 SS.hold @15:48:50.55 Intake @14.8psia, vacuum from 9.3 to 12.3psi @15:48:50.57 SS.reconfigure :inhaling 0.57ml/s @15:48:50.61 SS.seek 25ml @15:49:05.22 Stroke completed -- waiting for pressure to equalize ... @15:49:05.23 SS.reconfigure :exhale @15:49:05.27 SS.hold @15:49:17.82 Intake.select :close @15:49:17.96 SSV.dial :exhaust @15:49:18.71 Exhaust.select :open @15:49:18.82 SS.seek 0ml @15:49:27.86 Exhaust.select :close @15:49:27.96 SSV.dial CBV @15:49:27.99
CBV.dial SSV @15:49:28.74 Allowing 130s for 25.0ml of remaining 325.0ml @15:49:28.75 Intake.select :open @15:49:30.90 Intake @15.6psia, vacuum from 10.0 to 13.0psi @15:49:30.92 SS.reconfigure :inhaling 0.57ml/s @15:49:30.96 SS.seek 25ml @15:50:08.57 Vacuum too high -- waiting up to 40 seconds @15:50:08.58 SS.reconfigure :exhale @15:50:08.62 SS.hold @15:50:17.17 Intake @15.0psia, vacuum from 9.5 to 12.5psi @15:50:17.19 SS.reconfigure :inhaling 0.51ml/s @15:50:17.23 SS.seek 25ml @15:50:27.27 Stroke completed -- waiting for pressure to equalize ... @15:50:27.28 SS.reconfigure :exhale @15:50:27.32 SS.hold @15:50:39.85 Intake.select :close @15:50:39.99 SSV.dial :exhaust @15:50:40.74 Exhaust.select :open @15:50:40.84 SS.seek 0ml @15:50:49.81 Exhaust.select :close @15:50:49.92 SSV.dial CBV @15:50:49.97
CBV.dial SSV @15:50:50.68 Allowing 130s for 25.0ml of remaining 300.0ml @15:50:50.69 Intake.select :open @15:50:52.84 Intake @15.7psia, vacuum from 10.0 to 13.0psi @15:50:52.86 SS.reconfigure :inhaling 0.51ml/s @15:50:52.90 SS.seek 25ml @15:51:34.60 Vacuum too high -- waiting up to 40 seconds @15:51:34.61 SS.reconfigure :exhale @15:51:34.65 SS.hold @15:51:43.09 Intake @15.0psia, vacuum from 9.5 to 12.5psi @15:51:43.10 SS.reconfigure :inhaling 0.47ml/s @15:51:43.15 SS.seek 25ml @15:51:54.42 Stroke completed -- waiting for pressure to equalize ... @15:51:54.43 SS.reconfigure :exhale @15:51:54.47 SS.hold @15:52:08.82 Intake.select :close @15:52:08.96 SSV.dial :exhaust @15:52:09.70 Exhaust.select :open @15:52:09.81 SS.seek 0ml @15:52:18.62 Exhaust.select :close @15:52:18.73 SSV.dial CBV @15:52:18.76
CBV.dial SSV @15:52:19.50 Allowing 130s for 25.0ml of remaining 275.0ml @15:52:19.51 Intake.select :open @15:52:21.67 Intake @15.6psia, vacuum from 10.0 to 13.0psi @15:52:21.69 SS.reconfigure :inhaling 0.47ml/s @15:52:21.73 SS.seek 25ml @15:53:04.41 Vacuum too high -- waiting up to 40 seconds @15:53:04.42 SS.reconfigure :exhale @15:53:04.46 SS.hold @15:53:14.10 Intake @15.0psia, vacuum from 9.5 to 12.5psi @15:53:14.12 SS.reconfigure :inhaling 0.43ml/s @15:53:14.16 SS.seek 25ml @15:53:29.95 Stroke completed -- waiting for pressure to equalize ... @15:53:29.96 SS.reconfigure :exhale @15:53:30.05 SS.hold @15:53:46.31 Intake.select :close @15:53:46.44 SSV.dial :exhaust @15:53:47.19 Exhaust.select :open @15:53:47.29 SS.seek 0ml @15:53:56.11 Exhaust.select :close @15:53:56.21 SSV.dial CBV @15:53:56.23
CBV.dial SSV @15:53:57.00 Allowing 130s for 25.0ml of remaining 250.0ml @15:53:57.01 Intake.select :open @15:53:59.16 Intake @15.6psia, vacuum from 10.0 to 13.0psi @15:53:59.18 SS.reconfigure :inhaling 0.43ml/s @15:53:59.22 SS.seek 25ml @15:54:58.26 Vacuum too high -- waiting up to 40 seconds @15:54:58.27 SS.reconfigure :exhale @15:54:58.31 SS.hold @15:55:10.85 Intake @15.2psia, vacuum from 9.7 to 12.7psi @15:55:10.87 SS.reconfigure :inhaling 0.38ml/s @15:55:10.91 SS.seek 25ml @15:55:18.32 Stroke completed -- waiting for pressure to equalize ... @15:55:18.33 SS.reconfigure :exhale @15:55:18.37 SS.hold @15:55:34.57 Intake.select :close @15:55:34.70 SSV.dial :exhaust @15:55:35.47 Exhaust.select :open @15:55:35.63 SS.seek 0ml @15:55:44.39 Exhaust.select :close @15:55:44.49 SSV.dial CBV @15:55:44.51
CBV.dial SSV @15:55:45.26 Allowing 130s for 25.0ml of remaining 225.0ml @15:55:45.27 Intake.select :open @15:55:47.43 Intake @15.6psia, vacuum from 10.0 to 13.0psi @15:55:47.45 SS.reconfigure :inhaling 0.38ml/s @15:55:47.49 SS.seek 25ml @15:56:58.19 Vacuum too high -- waiting up to 40 seconds @15:56:58.20 SS.reconfigure :exhale @15:56:58.24 SS.hold @15:57:10.78 Intake @15.1psia, vacuum from 9.6 to 12.6psi @15:57:10.80 SS.reconfigure :inhaling 0.34ml/s @15:57:10.84 SS.seek 25ml @15:57:17.10 Stroke completed -- waiting for pressure to equalize ... @15:57:17.11 SS.reconfigure :exhale @15:57:17.15 SS.hold @15:57:35.36 Intake.select :close @15:57:35.50 SSV.dial :exhaust @15:57:36.24 Exhaust.select :open @15:57:36.35 SS.seek 0ml @15:57:45.37 Exhaust.select :close @15:57:45.47 SSV.dial CBV @15:57:45.49
CBV.dial SSV @15:57:46.24 Allowing 130s for 25.0ml of remaining 200.0ml @15:57:46.25 Intake.select :open @15:57:48.41 Intake @15.5psia, vacuum from 10.0 to 13.0psi @15:57:48.43 SS.reconfigure :inhaling 0.34ml/s @15:57:48.47 SS.seek 25ml @15:59:18.45 Stroke completed -- waiting for pressure to equalize ... @15:59:18.46 SS.reconfigure :exhale @15:59:18.50 SS.hold @15:59:48.97 Intake.select :close @15:59:49.11 SSV.dial :exhaust @15:59:49.85 Exhaust.select :open @15:59:49.96 SS.seek 0ml @15:59:58.79 Exhaust.select :close @15:59:58.89 SSV.dial CBV @15:59:58.91
CBV.dial SSV @15:59:59.66 Allowing 130s for 25.0ml of remaining 175.0ml @15:59:59.67 Intake.select :open @16:00:01.83 Intake @15.5psia, vacuum from 10.0 to 13.0psi @16:00:02.23 SS.reconfigure :inhaling 0.34ml/s @16:00:02.36 SS.seek 25ml @16:01:17.68 Vacuum too high -- waiting up to 40 seconds @16:01:17.69 SS.reconfigure :exhale @16:01:17.74 SS.hold @16:01:33.94 Intake @15.2psia, vacuum from 9.7 to 12.7psi @16:01:33.95 SS.reconfigure :inhaling 0.31ml/s @16:01:34.00 SS.seek 25ml @16:01:50.96 Stroke completed -- waiting for pressure to equalize ... @16:01:50.97 SS.reconfigure :exhale @16:01:51.01 SS.hold @16:02:09.68 SS.reconfigure :default @16:02:09.72 SS.hold @16:02:09.83 Intake.select :close @16:02:09.97 ShallowSampler::Clogged in MAIN -- Filtering too Slowly after 850.0ml @16:02:09.98 SSV.dial :exhaust @16:02:10.72 Exhaust.select :open @16:02:10.83 SS.reconfigure :exhale @16:02:10.87 SS.seek 0ml @16:02:20.03 Exhaust.select :close @16:02:20.14 Sampled 850.0ml @16:02:20.15 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @16:02:20.16 Intake.select :close @16:02:20.22 CSV.dial :waste @16:02:20.26 CS.seek 0ml @16:02:20.36 CSV.dial :air @16:02:20.39 SSV.dial CBV,avoiding: :exhaust @16:02:20.42
CBV.dial SSV @16:02:21.16 CS.seek 10ml @16:02:22.30
CTV.dial :air @16:02:23.08 delay 15 seconds @16:02:38.10 SSV.dial :spit,avoiding: :exhaust @16:02:39.40 CTV.dial CSV,avoiding: :intake @16:02:39.44
SS.seek 0ml @16:02:39.55 SSV.dial CBV,avoiding: :exhaust @16:02:45.43 CBV.dial between SSV and waste2 @16:02:45.81 CTV.dial CSV,avoiding: :air @16:02:45.83
CSV.dial CTV @16:02:46.27 CS.reconfigure :pressurize @16:02:46.31 CS.seek 0ml @16:03:04.91 BubblePoint: Intake @39.1psia, Outlet @14.9psia @16:03:04.92 CBV.dial SSV @16:03:05.24 SS.reconfigure :default @16:03:05.28 SS.seek 10ml @16:03:10.75 delay 15 seconds @16:03:25.87 Evacuated: Intake @18.1psia, Outlet @9.1psia @16:03:25.88 SSV.dial :spit,avoiding: :exhaust @16:03:27.20 SS.reconfigure :exhale @16:03:27.24 SS.seek 0ml @16:03:32.45 SS.reconfigure :fast @16:03:32.49 SS.seek 5ml @16:03:35.54 SSV.dial CBV,avoiding: :exhaust @16:03:35.56
CBV.dial SSV @16:03:36.87 CTV.dial :air,avoiding: :intake @16:03:38.20 CSV.dial CTV @16:03:38.24
SS.seek 10ml @16:03:39.23 CSV.dial :air @16:03:39.69 CS.reconfigure :fast @16:03:39.73 CS.seek 0ml @16:03:41.08
SSV.dial :spit,avoiding: :exhaust @16:03:42.38 SS.reconfigure :exhale @16:03:42.43 SS.seek 0ml @16:03:47.27 CS.stop @16:03:47.91
SS.reconfigure :fast @16:03:48.03 SS.seek 5ml @16:03:51.09 SSV.dial CBV,avoiding: :exhaust @16:03:51.11
CBV.dial SSV @16:03:53.43 SSV.dial :spit,avoiding: :exhaust @16:03:54.74 SS.reconfigure :default @16:03:54.78 SS.seek 0ml @16:03:57.86 SS.stop @16:03:57.90 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @16:03:57.91 Intake.select :close @16:03:57.98 CSV.dial :waste @16:03:58.10 SSV.dial CBV,avoiding: :exhaust @16:03:58.11
CBV.dial SSV @16:03:58.75 CS.seek 0ml @16:03:58.82 CSV.dial :air @16:03:59.43
CTV.dial :air @16:03:59.44 delay 15 seconds @16:03:59.55 CS.seek 10ml @16:04:14.46
SSV.dial :spit,avoiding: :exhaust @16:04:15.76 CTV.dial CSV,avoiding: :intake @16:04:15.79
SS.reconfigure :exhale @16:04:15.88 SS.seek 0ml @16:04:16.27 SSV.dial CBV,avoiding: :exhaust @16:04:23.89 CBV.dial between SSV and waste2 @16:04:24.29 CTV.dial CSV,avoiding: :air @16:04:24.32
CSV.dial CTV @16:04:24.75 CS.reconfigure :pressurize @16:04:24.80 CS.seek 0ml @16:04:45.78 BubblePoint: Intake @37.9psia, Outlet @13.8psia @16:04:45.79 CBV.dial SSV @16:04:46.12 SS.reconfigure :default @16:04:46.17 SS.seek 10ml @16:04:51.73 delay 15 seconds @16:05:06.85 Evacuated: Intake @19.8psia, Outlet @10.7psia @16:05:06.86 SSV.dial :spit,avoiding: :exhaust @16:05:08.16 SS.reconfigure :exhale @16:05:08.20 SS.seek 0ml @16:05:13.82 SS.reconfigure :fast @16:05:13.86 SS.seek 5ml @16:05:16.48 SSV.dial CBV,avoiding: :exhaust @16:05:16.49
CBV.dial SSV @16:05:17.80 CTV.dial :air,avoiding: :intake @16:05:19.12 CSV.dial CTV @16:05:19.16
SS.seek 10ml @16:05:20.16 CSV.dial :air @16:05:20.61 CS.reconfigure :fast @16:05:20.65 CS.seek 0ml @16:05:21.60
SSV.dial :spit,avoiding: :exhaust @16:05:22.91 SS.reconfigure :exhale @16:05:22.95 SS.seek 0ml @16:05:26.05 CS.stop @16:05:27.79
SS.reconfigure :fast @16:05:27.83 SS.seek 5ml @16:05:30.70 SSV.dial CBV,avoiding: :exhaust @16:05:30.71
CBV.dial SSV @16:05:33.03 SSV.dial :spit,avoiding: :exhaust @16:05:34.32 SS.reconfigure :default @16:05:34.36 SS.seek 0ml @16:05:37.61 SS.stop @16:05:37.64 Lysing: Adding 2.0ml AqMeOH @16:05:37.65 CBV.dial between CSV and waste1,avoiding: :DBV @16:05:39.14 CTV.dial CSV @16:05:40.45 CSV.dial CSR @16:05:41.22 Pulling 1ml of CSR.AqMeOH @16:05:41.23 CSR.select :AqMeOH @16:05:41.33 CS.reconfigure :default @16:05:41.37 CS.seek 1ml @16:05:44.78 delay 5 seconds @16:05:49.79 CSR.select :air @16:05:49.95 CS.seek 6ml @16:06:03.59 delay 2 seconds @16:06:05.60 CSV.dial :waste2 @16:06:06.03 CS.reconfigure :fast @16:06:06.07 CS.seek 0ml @16:06:20.37 CSV.dial CSR @16:06:20.86 Pulling 2ml of CSR.AqMeOH @16:06:20.87 CSR.select :AqMeOH @16:06:20.97 CS.reconfigure :default @16:06:21.02 CS.seek 2ml @16:06:26.93 delay 5 seconds @16:06:31.95 CSR.select :air @16:06:32.10 CS.seek 5ml @16:06:40.77 delay 2 seconds @16:06:42.79 CSV.dial CTV @16:06:43.22 CS.seek 1ml @16:06:54.56 Before lysing Collection Puck Heater at 19.9C/39.5psia @16:06:54.58 Heating Puck.from 2, :sh1 in Collection Clamp to 70C for 10:00 @16:06:54.60 CH.seek 70C,8:00 @16:08:46.08 CH.hold 10:00,70C @16:08:46.25 Begun lysing Collection Puck Heater at 68C/46.8psia @16:18:46.26 Ended lysing Collection Puck Heater at 70C/45.5psia @16:18:46.27 CS.seek 0ml @16:18:49.61 CTV.dial between spare and air,avoiding: :intake @16:18:50.64 CSV.dial :air @16:18:51.08 CS.seek 5ml @16:19:04.70 CSV.dial CTV @16:19:05.14 delay 2 seconds @16:19:07.15 CSV.dial :air @16:19:07.61 delay 2 seconds @16:19:09.62 CSV.dial CTV @16:19:10.08 delay 2 seconds @16:19:12.10 CSV.dial :air @16:19:12.54 delay 2 seconds @16:19:14.56 CS.seek 0ml @16:19:28.28 Puck @72.7psia after final lysis pressurization @16:19:28.29 Recovering Lysate into Collection syringe @16:19:28.30 CSV.dial CBV @16:19:29.29 CBV.dial CSV @16:19:29.65 CS.seek 5ml @16:19:43.63 delay 5 seconds @16:19:48.65 CTV.dial :air @16:19:49.18 CS.seek 7ml @16:19:55.04 delay 5 seconds @16:20:00.06 CSV.dial :air2 @16:20:00.50 CS.seek 10ml @16:20:09.07 CSV.dial between air and CTV,avoiding: CBV @16:20:10.22 Lysing Complete @16:20:10.29 Storing sh1 puck in Collection Clamp to tube 1 @16:20:10.44 SC.reconfigure :tight @16:20:10.49 SC.seek 1 @16:20:14.13 SC.configure :limp @16:20:14.32 Elbow.reconfigure :tight @16:20:14.36 Elbow.seek 1 @16:20:16.13 Forearm.reconfigure :tight @16:20:16.17 Forearm.seek 1 + 450 counts @16:20:17.27 SE.reconfigure :pick @16:20:17.32 SE.seek :beamBreak @16:20:19.50 SE.configure :default @16:20:19.64 Elbow.reconfigure :limp @16:20:19.69 Forearm.reconfigure :loose @16:20:19.72 Forearm.seek 1 @16:20:19.94 Elbow.hold @16:20:20.00 Elbow.reconfigure :tight @16:20:20.09 SE.seek 15.1 Pucks below Top Plate @16:20:20.55 Forearm.force -20 @16:20:20.58 Forearm.seek 1 + 297 counts @16:20:21.36 Forearm.reconfigure :tight @16:20:21.40 Forearm.seek 1 + 450 counts @16:20:21.61 SE.seek 16.1 Pucks below Top Plate @16:20:22.21 Forearm.reconfigure :loose @16:20:22.25 Forearm.seek :retract @16:20:23.18 Elbow.seek CC @16:20:24.83 Elbow.reconfigure :loose @16:20:24.92 Forearm.seek CC @16:20:25.91 Hand.close @16:20:26.51 CC.seek :open @16:20:31.22 Forearm.seek :retract @16:20:32.20 Elbow.reconfigure :tight @16:20:32.24 Elbow.seek 1 @16:20:33.99 Elbow.reconfigure :limp @16:20:34.03 Forearm.seek 1 @16:20:35.01 Elbow.hold @16:20:35.07 Elbow.reconfigure :tight @16:20:35.11 Hand.open @16:20:35.87 Forearm.force -20 @16:20:35.90 Forearm.seek 1 + 317 counts @16:20:36.22 Forearm.reconfigure :tight @16:20:36.27 Forearm.seek 1 + 450 counts @16:20:36.58 SE.seek :down @16:20:37.70
Forearm.reconfigure :loose @16:20:37.74 Forearm.seek :retract @16:20:38.19 SE.stop @16:20:38.67
Tranferring DA @16:20:38.68 Beginning samplePassC @16:20:38.69 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @16:20:38.87 Elbow.seek FlushPuck::Garage @16:20:40.67 Elbow.reconfigure :loose @16:20:40.76 Forearm.seek :garage @16:20:41.74 Hand.close @16:20:42.49 Forearm.seek :retract @16:20:43.46 Elbow.reconfigure :tight @16:20:43.50 Elbow.seek CC @16:20:46.47 Elbow.reconfigure :loose @16:20:46.56 Forearm.reconfigure :tight @16:20:46.61 Forearm.seek CC @16:20:47.83 CC.seek :closed @16:20:52.34 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.25 @16:20:52.41 Hand.open @16:20:53.07 Forearm.reconfigure :loose @16:20:53.11 Forearm.seek :retract @16:20:54.07 PSV.dial PSR @16:20:55.45 Pulling 1.5ml of PSR.flush @16:20:55.46 PSR.select :flush @16:20:55.57 PS.seek 1.5ml @16:21:11.77 delay 5 seconds @16:21:16.79 PSV.dial :waste1 @16:21:17.24 PS.seek 0ml @16:21:33.36 PSV.dial PSR @16:21:33.81 PSR.select :air @16:21:33.92 PS.seek 2.5ml @16:22:00.36 PRV.dial CBV,avoiding: :ab2 @16:22:00.38
CBV.dial PRV @16:22:02.06 PSV.dial :mixloop @16:22:03.09 CTV.dial :air,avoiding: :intake @16:22:03.11 PTV.dial :PRVmixing @16:22:04.41 PS.seek 0ml @16:22:30.70 PSV.dial :air @16:22:31.16 PS.seek 2.5ml @16:22:57.54 PSV.dial :mixloop @16:22:58.00 PS.seek 0ml @16:23:24.86 PSV.dial :air @16:23:25.32 PS.seek 2.5ml @16:23:51.73 PSV.dial :mixloop @16:23:52.19 PS.seek 0ml @16:24:18.52 Garaging FlushPuck in Collection Clamp @16:24:18.59 Elbow.reconfigure :tight @16:24:18.63 Elbow.seek CC @16:24:19.00 Elbow.reconfigure :loose @16:24:19.09 Forearm.seek CC @16:24:20.13 Hand.close @16:24:20.76 CC.seek :open @16:24:25.36 Forearm.seek :retract @16:24:26.33 Elbow.reconfigure :tight @16:24:26.38 Elbow.seek :garage @16:24:29.21 Elbow.reconfigure :loose @16:24:29.31 Forearm.reconfigure :tight @16:24:29.35 Forearm.seek :garage @16:24:30.38 Hand.open @16:24:31.06 Forearm.reconfigure :loose @16:24:31.10 Forearm.seek :retract @16:24:32.06 PSV.dial :mixloop @16:24:32.08 PTV.dial :PRVmixing @16:24:32.09 PRV.dial CBV @16:24:32.11
CBV.dial PRV @16:24:32.13 CTV.dial :air,avoiding: :intake @16:24:32.15 Loading lyfil puck from tube 2 into Collection Clamp @16:24:32.36 SC.reconfigure :tight @16:24:32.41 SC.seek 2 @16:24:35.98 SC.configure :limp @16:24:36.17 Elbow.reconfigure :tight @16:24:36.21 Elbow.seek 2 @16:24:38.12 Forearm.reconfigure :tight @16:24:38.16 Forearm.seek 2 + 450 counts @16:24:39.17 SE.reconfigure :pick @16:24:39.22 SE.seek :beamBreak @16:24:41.33 SE.configure :default @16:24:41.47 Elbow.seek 2 @16:24:41.65 Forearm.seek 2 + 300 counts @16:24:41.85 Forearm.reconfigure :loose @16:24:41.89 Hand.close @16:24:42.52 SE.seek :down @16:24:42.72
Forearm.seek :retract @16:24:43.66 Elbow.seek CC @16:24:44.29 SE.stop @16:24:45.31
Elbow.reconfigure :loose @16:24:45.40 Forearm.reconfigure :tight @16:24:45.44 Forearm.seek CC @16:24:46.70 CC.seek :closed @16:24:51.21 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.06 @16:24:51.22 Hand.open @16:24:51.94 Forearm.reconfigure :loose @16:24:51.98 Forearm.seek :retract @16:24:52.94 CTV.dial CSV,avoiding: :intake @16:24:54.29 CSV.dial CTV @16:24:54.59 PS.seek 2.5ml @16:25:20.95 CS.seek 4.501ml @16:25:35.88 delay 8 seconds @16:25:43.90 PSV.dial :air @16:25:44.36 delay 2 seconds @16:25:46.37 PSV.dial :waste2 @16:25:47.09 PS.seek 0ml @16:26:13.48 CBV.dial between PRV and waste1 @16:26:13.80 CSV.dial :air @16:26:14.26 CS.seek 10ml @16:26:29.56 CSV.dial CTV @16:26:30.01 CS.seek 0ml @16:26:56.66 CBV.dial :waste1 @16:26:56.96 delay 5 seconds @16:27:01.98 CTV.dial :air,avoiding: :intake @16:27:03.37 Storing lyfil puck in Collection Clamp to tube 1 @16:27:03.54 SC.reconfigure :tight @16:27:03.58 SC.seek 1 @16:27:07.17 SC.configure :limp @16:27:07.36 Elbow.reconfigure :tight @16:27:07.40 Elbow.seek 1 @16:27:09.19 Forearm.reconfigure :tight @16:27:09.24 Forearm.seek 1 + 450 counts @16:27:10.25 SE.reconfigure :pick @16:27:10.30 SE.seek :beamBreak @16:27:12.31 SE.configure :default @16:27:12.46 Elbow.reconfigure :limp @16:27:12.50 Forearm.reconfigure :loose @16:27:12.54 Forearm.seek 1 @16:27:12.78 Elbow.hold @16:27:12.85 Elbow.reconfigure :tight @16:27:12.93 SE.seek 16.1 Pucks below Top Plate @16:27:13.39 Forearm.force -20 @16:27:13.42 Forearm.seek 1 + 305 counts @16:27:14.07 Forearm.reconfigure :tight @16:27:14.12 Forearm.seek 1 + 450 counts @16:27:14.32 SE.seek 17.1 Pucks below Top Plate @16:27:14.91 Forearm.reconfigure :loose @16:27:14.95 Forearm.seek :retract @16:27:15.91 Elbow.seek CC @16:27:17.59 Elbow.reconfigure :loose @16:27:17.67 Forearm.seek CC @16:27:18.67 Hand.close @16:27:19.25 CC.seek :open @16:27:23.96 Forearm.seek :retract @16:27:24.94 Elbow.reconfigure :tight @16:27:24.98 Elbow.seek 1 @16:27:26.74 Elbow.reconfigure :limp @16:27:26.78 Forearm.seek 1 @16:27:27.80 Elbow.hold @16:27:27.86 Elbow.reconfigure :tight @16:27:27.91 Hand.open @16:27:28.62 Forearm.force -20 @16:27:28.65 Forearm.seek 1 + 319 counts @16:27:28.96 Forearm.reconfigure :tight @16:27:29.01 Forearm.seek 1 + 450 counts @16:27:29.32 SE.seek :down @16:27:30.44
Forearm.reconfigure :loose @16:27:30.48 Forearm.seek :retract @16:27:30.85 SE.stop @16:27:31.39
PSV.dial :air @16:27:32.14 PS.seek 2.5ml @16:27:58.57 delay 1 second @16:27:59.58 PSV.dial :mixloop @16:28:00.01 delay 1 second @16:28:01.02 PSV.dial :air @16:28:01.49 delay 1 second @16:28:02.51 PSV.dial :mixloop @16:28:02.96 delay 1 second @16:28:03.98 PSV.dial :air @16:28:04.43 delay 1 second @16:28:05.45 PS.seek 0ml @16:28:31.82 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @16:28:32.00 Elbow.seek FlushPuck::Garage @16:28:33.79 Elbow.reconfigure :loose @16:28:33.88 Forearm.seek :garage @16:28:34.86 Hand.close @16:28:36.06 Forearm.seek :retract @16:28:37.02 Elbow.reconfigure :tight @16:28:37.06 Elbow.seek PC @16:28:38.31 Elbow.reconfigure :loose @16:28:38.40 Forearm.reconfigure :tight @16:28:38.44 Forearm.seek PC @16:28:39.88 PC.seek :closed @16:28:44.51 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.58 @16:28:44.57 Hand.open @16:28:45.18 Forearm.reconfigure :loose @16:28:45.23 Forearm.seek :retract @16:28:46.17 PRV.dial :air @16:28:46.62 PSV.dial :mixloop @16:28:47.15 PS.seek 0.3ml @16:28:51.00 PSV.dial PSR @16:28:52.10 Pulling 0.4ml of PSR.air @16:28:52.11 PSR.select :air @16:28:52.13 PS.seek 0.7ml @16:28:57.04 delay 3 seconds @16:29:00.05 PSR.select :air @16:29:00.08 PS.seek 2.5ml @16:29:19.26 delay 3 seconds @16:29:22.27 PSV.dial :mixloop @16:29:23.32 PTV.dial :mixing @16:29:24.05 PBV.dial :waste2 @16:29:24.98 PS.seek 0ml @16:29:51.35 Garaging FlushPuck in Processing Clamp @16:29:51.42 Elbow.reconfigure :tight @16:29:51.46 Elbow.seek PC @16:29:51.57 Elbow.reconfigure :loose @16:29:51.65 Forearm.seek PC @16:29:52.61 Hand.close @16:29:53.25 PC.seek :open @16:29:57.96 Forearm.seek :retract @16:29:58.91 Elbow.reconfigure :tight @16:29:58.95 Elbow.seek :garage @16:30:00.45 Elbow.reconfigure :loose @16:30:00.53 Forearm.reconfigure :tight @16:30:00.58 Forearm.seek :garage @16:30:01.59 Hand.open @16:30:02.31 Forearm.reconfigure :loose @16:30:02.35 Forearm.seek :retract @16:30:03.30 Loading da puck from tube 2 into Processing Clamp @16:30:03.52 SC.reconfigure :tight @16:30:03.56 SC.seek 2 @16:30:07.12 SC.configure :limp @16:30:07.31 Elbow.reconfigure :tight @16:30:07.35 Elbow.seek 2 @16:30:09.24 Forearm.reconfigure :tight @16:30:09.28 Forearm.seek 2 + 450 counts @16:30:10.26 SE.reconfigure :pick @16:30:10.31 SE.seek :beamBreak @16:30:12.54 SE.configure :default @16:30:12.68 Elbow.seek 2 @16:30:12.85 Forearm.seek 2 + 300 counts @16:30:13.15 Forearm.reconfigure :loose @16:30:13.19 Hand.close @16:30:13.77 SE.seek :down @16:30:13.96
Forearm.seek :retract @16:30:14.95 Elbow.seek PC @16:30:15.60 SE.stop @16:30:17.66
Elbow.reconfigure :loose @16:30:17.76 Forearm.reconfigure :tight @16:30:17.80 Forearm.seek PC @16:30:18.97 PC.seek :closed @16:30:23.60 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.58 @16:30:23.61 Hand.open @16:30:24.31 Forearm.reconfigure :loose @16:30:24.35 Forearm.seek :retract @16:30:25.30 Heating Puck.from 2, :da in Processing Clamp to 28C for 2:00:00 @16:30:25.31 PH.seek 28C,5:00 @16:30:56.79 PH.hold 2:00:00,28C @16:30:56.84 delay 1:30 @16:32:26.88 PSR.select :air @16:32:26.90 PSV.dial PSR @16:32:27.99 Pulling 0.5ml of PSR.wash @16:32:28.00 PSR.select :wash @16:32:28.12 PS.seek 0.5ml @16:32:34.04 delay 5 seconds @16:32:39.05 PSR.select :air @16:32:39.17 PS.seek 2ml @16:32:55.34 PTV.dial :puck @16:32:56.06 PSV.dial PTV @16:32:56.94 PBV.dial :waste1 @16:32:58.33 PS.seek 1.5ml @16:33:04.19 delay 3 seconds @16:33:07.21 PBV.dial between PSV and waste1 @16:33:07.57 PS.seek 0ml @16:33:23.72 delay 5:00 @16:38:23.74 recoverPSRreagent08 @16:38:23.75 PTV.dial :puck @16:38:23.76 PBV.dial :waste1 @16:38:24.12 delay 30 seconds @16:38:54.14 PBV.dial between PSV and waste1 @16:38:54.51 PSR.select :air @16:38:54.53 PSV.dial PSR @16:38:55.27 PS.reconfigure :fast @16:38:55.31 PS.seek 2.5ml @16:39:18.71 PSV.dial PTV @16:39:19.45 delay 5 seconds @16:39:24.47 PSV.dial PSR @16:39:25.21 delay 5 seconds @16:39:30.23 PSV.dial PTV @16:39:30.98 delay 5 seconds @16:39:36.00 PSV.dial :air @16:39:36.75 PS.seek 0ml @16:39:59.65 PTV.dial :PRVmixing,via: PRV @16:40:00.96 PSV.dial :mixloop @16:40:01.43 PRV.dial :ab1 @16:40:02.17 PS.reconfigure :slow @16:40:02.21 PS.seek 0.15ml @16:40:05.84 delay 2 seconds @16:40:07.85 CBV.dial PRV @16:40:07.87
PRV.dial CBV @16:40:08.44 PS.seek 0.35ml @16:40:12.99 delay 2 seconds @16:40:15.01 CBV.dial between PRV and PSV @16:40:15.32 PRV.dial :air @16:40:15.77 PS.seek 0.9ml @16:40:27.49 PRV.dial :waste2 @16:40:28.22 PS.seek 0.5ml @16:40:36.90 addPMLReagent08 @16:40:36.91 PSV.dial :air @16:40:37.37 PS.reconfigure :default @16:40:37.41 PS.seek 2ml @16:40:53.52 PSV.dial :PML @16:40:53.99 PTV.dial :mixing @16:40:54.73 PBV.dial :waste1 @16:40:55.15 PS.seek 1.85ml @16:40:57.49 PBV.dial between PSV and waste1 @16:40:57.87 PS.seek 0ml @16:41:17.74 delay 2 seconds @16:41:19.76 Beginning samplePassC @16:41:19.77 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @16:41:19.94 Elbow.reconfigure :tight @16:41:19.98 Elbow.seek FlushPuck::Garage @16:41:21.29 Elbow.reconfigure :loose @16:41:21.39 Forearm.seek :garage @16:41:22.35 Hand.close @16:41:23.61 Forearm.seek :retract @16:41:24.54 Elbow.reconfigure :tight @16:41:24.58 Elbow.seek CC @16:41:27.45 Elbow.reconfigure :loose @16:41:27.55 Forearm.reconfigure :tight @16:41:27.59 Forearm.seek CC @16:41:28.86 CC.seek :closed @16:41:33.40 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.35 @16:41:33.46 Hand.open @16:41:34.18 Forearm.reconfigure :loose @16:41:34.22 Forearm.seek :retract @16:41:35.16 PSV.dial PSR @16:41:36.25 Pulling 1.5ml of PSR.flush @16:41:36.26 PSR.select :flush @16:41:36.37 PS.seek 1.5ml @16:41:52.46 delay 5 seconds @16:41:57.48 PSV.dial :waste1 @16:41:57.93 PS.seek 0ml @16:42:14.08 PSV.dial PSR @16:42:14.54 PSR.select :air @16:42:14.65 PS.seek 2.5ml @16:42:41.00 PRV.dial CBV,avoiding: :ab2 @16:42:41.01
CBV.dial PRV @16:42:42.65 PSV.dial :mixloop @16:42:43.68 CTV.dial :air,avoiding: :intake @16:42:43.69 PTV.dial :PRVmixing @16:42:44.45 PS.seek 0ml @16:43:10.74 PSV.dial :air @16:43:11.18 PS.seek 2.5ml @16:43:37.52 PSV.dial :mixloop @16:43:37.98 PS.seek 0ml @16:44:04.30 PSV.dial :air @16:44:04.76 PS.seek 2.5ml @16:44:31.17 PSV.dial :mixloop @16:44:31.65 PS.seek 0ml @16:44:57.99 Garaging FlushPuck in Collection Clamp @16:44:58.06 Elbow.reconfigure :tight @16:44:58.10 Elbow.seek CC @16:44:58.45 Elbow.reconfigure :loose @16:44:58.54 Forearm.seek CC @16:44:59.50 Hand.close @16:45:00.11 CC.seek :open @16:45:04.75 Forearm.seek :retract @16:45:05.75 Elbow.reconfigure :tight @16:45:05.80 Elbow.seek :garage @16:45:08.68 Elbow.reconfigure :loose @16:45:08.77 Forearm.reconfigure :tight @16:45:08.81 Forearm.seek :garage @16:45:09.85 Hand.open @16:45:10.55 Forearm.reconfigure :loose @16:45:10.59 Forearm.seek :retract @16:45:11.57 delayUntil "16:49:19.75" @16:45:11.59 Whole Cell Archive sampling 1000ml @16:45:11.61 Loading wcr puck from tube 2 into Collection Clamp @16:45:11.89 SC.reconfigure :tight @16:45:11.94 SC.seek 2 @16:45:12.26 SC.configure :limp @16:45:12.48 Elbow.reconfigure :tight @16:45:12.52 Elbow.seek 2 @16:45:14.53 Forearm.reconfigure :tight @16:45:14.57 Forearm.seek 2 + 450 counts @16:45:15.61 SE.reconfigure :pick @16:45:15.66 SE.seek :beamBreak @16:45:18.43 SE.configure :default @16:45:18.60 Elbow.seek 2 @16:45:18.78 Forearm.seek 2 + 300 counts @16:45:18.94 Forearm.reconfigure :loose @16:45:18.98 Hand.close @16:45:19.63 SE.seek :down @16:45:19.82 Forearm.seek :retract @16:45:20.85 Elbow.seek CC @16:45:21.51 SE.stop @16:45:22.52 Elbow.reconfigure :loose @16:45:22.61 Forearm.reconfigure :tight @16:45:22.66 Forearm.seek CC @16:45:23.83 CC.seek :closed @16:45:28.36 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.14 @16:45:28.37 Hand.open @16:45:29.05 Forearm.reconfigure :loose @16:45:29.09 Forearm.seek :retract @16:45:30.07 collectSample 1000ml w/bubble point of 35psi @16:45:30.08 Priming 7ml @16:45:30.09 Intake.select :close @16:45:30.10 Exhaust.select :close @16:45:30.11 SS.hold @16:45:30.31 Calibrating pressure sensors @16:45:30.32 CTV.dial :air @16:45:30.33 SSV.dial :spit,avoiding: :exhaust @16:45:30.35 SS.reconfigure :fast @16:45:30.39 SS.seek 5ml @16:45:33.11 SSV.dial CBV @16:45:33.13 CBV.dial SSV @16:45:34.47 delay 3.8 seconds @16:45:38.39 SSV.dial :spit @16:45:39.71 SS.seek 0ml @16:45:42.53 SSV.dial CBV @16:45:43.86 CTV.dial :intake @16:45:44.61 SSV.dial CBV @16:45:44.64 CBV.dial SSV @16:45:44.65 Allowing 40s for 7.0ml of remaining 7.0ml @16:45:44.66 Intake.select :open @16:45:46.82 Intake @15.2psia, vacuum from 9.7 to 12.7psi @16:45:46.84 SS.reconfigure :inhaling 2.20ml/s @16:45:46.88 SS.seek 7ml @16:45:54.07 Stroke completed -- waiting for pressure to equalize ... @16:45:54.08 SS.reconfigure :exhale @16:45:54.13 SS.hold @16:45:58.20 Intake.select :close @16:45:58.35 SSV.dial :exhaust @16:45:59.11 Exhaust.select :open @16:45:59.22 SS.seek 0ml @16:46:03.32 Exhaust.select :close @16:46:03.43 Sampling 1000.0ml @16:46:03.44 Intake.select :close @16:46:03.45 Exhaust.select :close @16:46:03.46 SS.reconfigure :default @16:46:03.50 SS.hold @16:46:03.70 CTV.dial :intake @16:46:03.71 SSV.dial CBV @16:46:03.73 CBV.dial SSV @16:46:04.50 Allowing 130s for 25.0ml of remaining 1000.0ml @16:46:04.51 Intake.select :open @16:46:06.66 Intake @15.1psia, vacuum from 9.6 to 12.6psi @16:46:06.68 SS.reconfigure :inhaling 2.20ml/s @16:46:06.73 SS.seek 25ml @16:46:17.22 Vacuum too high -- waiting up to 40 seconds @16:46:17.23 SS.reconfigure :exhale @16:46:17.28 SS.hold @16:46:21.58 Intake @13.2psia, vacuum from 7.7 to 10.7psi @16:46:21.60 SS.reconfigure :inhaling 1.98ml/s @16:46:21.64 SS.seek 25ml @16:46:26.77 Stroke completed -- waiting for pressure to equalize ... @16:46:26.79 SS.reconfigure :exhale @16:46:26.83 SS.hold @16:46:32.40 Intake.select :close @16:46:32.54 SSV.dial :exhaust @16:46:33.31 Exhaust.select :open @16:46:33.42 SS.seek 0ml @16:46:42.43 Exhaust.select :close @16:46:42.54 SSV.dial CBV @16:46:42.57 CBV.dial SSV @16:46:43.32 Allowing 130s for 25.0ml of remaining 975.0ml @16:46:43.34 Intake.select :open @16:46:45.49 Intake @15.7psia, vacuum from 10.0 to 13.0psi @16:46:45.51 SS.reconfigure :inhaling 1.98ml/s @16:46:45.55 SS.seek 25ml @16:46:56.41 Vacuum too high -- waiting up to 40 seconds @16:46:56.42 SS.reconfigure :exhale @16:46:56.46 SS.hold @16:46:57.96 Intake @13.3psia, vacuum from 7.8 to 10.8psi @16:46:57.98 SS.reconfigure :inhaling 1.79ml/s @16:46:58.03 SS.seek 25ml @16:47:04.04 Stroke completed -- waiting for pressure to equalize ... @16:47:04.05 SS.reconfigure :exhale @16:47:04.09 SS.hold @16:47:10.50 Intake.select :close @16:47:10.64 SSV.dial :exhaust @16:47:11.38 Exhaust.select :open @16:47:11.50 SS.seek 0ml @16:47:20.24 Exhaust.select :close @16:47:20.35 SSV.dial CBV @16:47:20.37 CBV.dial SSV @16:47:21.14 Allowing 130s for 25.0ml of remaining 950.0ml @16:47:21.15 Intake.select :open @16:47:23.30 Intake @15.9psia, vacuum from 10.0 to 13.0psi @16:47:23.32 SS.reconfigure :inhaling 1.79ml/s @16:47:23.36 SS.seek 25ml @16:47:33.78 Vacuum too high -- waiting up to 40 seconds @16:47:33.79 SS.reconfigure :exhale @16:47:33.84 SS.hold @16:47:34.66 Intake @13.3psia, vacuum from 7.8 to 10.8psi @16:47:34.68 SS.reconfigure :inhaling 1.61ml/s @16:47:34.72 SS.seek 25ml @16:47:42.93 Stroke completed -- waiting for pressure to equalize ... @16:47:42.94 SS.reconfigure :exhale @16:47:42.98 SS.hold @16:47:50.45 Intake.select :close @16:47:50.59 SSV.dial :exhaust @16:47:51.35 Exhaust.select :open @16:47:51.46 SS.seek 0ml @16:48:00.44 Exhaust.select :close @16:48:00.55 SSV.dial CBV @16:48:00.57 CBV.dial SSV @16:48:01.34 Allowing 130s for 25.0ml of remaining 925.0ml @16:48:01.35 Intake.select :open @16:48:03.51 Intake @15.8psia, vacuum from 10.0 to 13.0psi @16:48:03.53 SS.reconfigure :inhaling 1.61ml/s @16:48:03.57 SS.seek 25ml @16:48:15.69 Vacuum too high -- waiting up to 40 seconds @16:48:15.70 SS.reconfigure :exhale @16:48:15.74 SS.hold @16:48:17.92 Intake @13.7psia, vacuum from 8.2 to 11.2psi @16:48:17.94 SS.reconfigure :inhaling 1.45ml/s @16:48:17.98 SS.seek 25ml @16:48:25.24 Stroke completed -- waiting for pressure to equalize ... @16:48:25.25 SS.reconfigure :exhale @16:48:25.30 SS.hold @16:48:31.42 Intake.select :close @16:48:31.56 SSV.dial :exhaust @16:48:32.31 Exhaust.select :open @16:48:32.42 SS.seek 0ml @16:48:41.19 Exhaust.select :close @16:48:41.30 SSV.dial CBV @16:48:41.32 CBV.dial SSV @16:48:42.09 Allowing 130s for 25.0ml of remaining 900.0ml @16:48:42.10 Intake.select :open @16:48:44.25 Intake @16.0psia, vacuum from 10.0 to 13.0psi @16:48:44.27 SS.reconfigure :inhaling 1.45ml/s @16:48:44.31 SS.seek 25ml @16:48:56.41 Vacuum too high -- waiting up to 40 seconds @16:48:56.42 SS.reconfigure :exhale @16:48:56.46 SS.hold @16:48:58.62 Intake @14.0psia, vacuum from 8.5 to 11.5psi @16:48:58.64 SS.reconfigure :inhaling 1.30ml/s @16:48:58.69 SS.seek 25ml @16:49:08.18 Stroke completed -- waiting for pressure to equalize ... @16:49:08.19 SS.reconfigure :exhale @16:49:08.23 SS.hold @16:49:14.73 Intake.select :close @16:49:14.88 SSV.dial :exhaust @16:49:15.62 Exhaust.select :open @16:49:15.73 SS.seek 0ml @16:49:19.76 recoverPRVreagent08 @16:49:19.77 PTV.dial :mixing @16:49:20.51 PBV.dial :waste1 @16:49:20.89 delay 30 seconds @16:49:24.79 Exhaust.select :close @16:49:24.90 SSV.dial CBV @16:49:24.92 CBV.dial SSV @16:49:25.68 Allowing 130s for 25.0ml of remaining 875.0ml @16:49:25.69 Intake.select :open @16:49:27.85 Intake @15.9psia, vacuum from 10.0 to 13.0psi @16:49:27.87 SS.reconfigure :inhaling 1.30ml/s @16:49:27.91 SS.seek 25ml @16:49:42.34 Vacuum too high -- waiting up to 40 seconds @16:49:42.35 SS.reconfigure :exhale @16:49:42.39 SS.hold @16:49:44.97 Intake @14.3psia, vacuum from 8.8 to 11.8psi @16:49:45.45 SS.reconfigure :inhaling 1.17ml/s @16:49:45.51 SS.seek 25ml @16:49:50.91 PBV.dial between PSV and waste1 @16:49:51.26 PSV.dial :air @16:49:51.86 PS.reconfigure :fast @16:49:51.91 PS.seek 2.5ml @16:49:54.41 Stroke completed -- waiting for pressure to equalize ... @16:49:54.42 SS.reconfigure :exhale @16:49:54.47 SS.hold @16:50:00.03 Intake.select :close @16:50:00.17 SSV.dial :exhaust @16:50:00.91 Exhaust.select :open @16:50:01.03 SS.seek 0ml @16:50:10.08 Exhaust.select :close @16:50:10.19 SSV.dial CBV @16:50:10.21 CBV.dial SSV @16:50:10.98 Allowing 130s for 25.0ml of remaining 850.0ml @16:50:10.99 Intake.select :open @16:50:13.14 Intake @15.9psia, vacuum from 10.0 to 13.0psi @16:50:13.16 SS.reconfigure :inhaling 1.17ml/s @16:50:13.20 SS.seek 25ml @16:50:15.60 PSV.dial :mixloop @16:50:16.06 delay 2 seconds @16:50:18.08 PSV.dial :air @16:50:18.55 delay 2 seconds @16:50:20.56 PSV.dial :mixloop @16:50:21.03 delay 2 seconds @16:50:23.04 PSV.dial :air @16:50:23.52 PS.seek 0ml @16:50:32.62 Vacuum too high -- waiting up to 40 seconds @16:50:32.63 SS.reconfigure :exhale @16:50:32.68 SS.hold @16:50:35.71 Intake @14.3psia, vacuum from 8.8 to 11.8psi @16:50:35.73 SS.reconfigure :inhaling 1.06ml/s @16:50:35.78 SS.seek 25ml @16:50:40.66 Stroke completed -- waiting for pressure to equalize ... @16:50:40.67 SS.reconfigure :exhale @16:50:40.71 SS.hold @16:50:46.54 washCycleDA #1 of 2 @16:50:46.55 PSR.select :air @16:50:46.57 PSV.dial PSR @16:50:47.13 Intake.select :close @16:50:47.27 SSV.dial :exhaust @16:50:47.92 Pulling 0.5ml of PSR.wash @16:50:47.93 PSR.select :wash @16:50:48.04 Exhaust.select :open @16:50:48.10 PS.reconfigure :default @16:50:48.14 PS.seek 0.5ml @16:50:48.19 SS.seek 0ml @16:50:54.14 delay 5 seconds @16:50:56.96 Exhaust.select :close @16:50:57.08 SSV.dial CBV @16:50:57.11 CBV.dial SSV @16:50:57.87 Allowing 130s for 25.0ml of remaining 825.0ml @16:50:57.88 Intake.select :open @16:50:59.15 PSR.select :air @16:50:59.26 PS.seek 2ml @16:51:00.03 Intake @15.9psia, vacuum from 10.0 to 13.0psi @16:51:00.05 SS.reconfigure :inhaling 1.06ml/s @16:51:00.09 SS.seek 25ml @16:51:15.35 PTV.dial :puck @16:51:16.09 PSV.dial PTV @16:51:16.84 PBV.dial :waste1 @16:51:17.24 PS.seek 1.5ml @16:51:23.14 delay 3 seconds @16:51:23.81 Vacuum too high -- waiting up to 40 seconds @16:51:23.82 SS.reconfigure :exhale @16:51:23.87 SS.hold @16:51:26.15 PBV.dial between PSV and waste1 @16:51:26.59 PS.seek 0ml @16:51:27.66 Intake @14.5psia, vacuum from 9.0 to 12.0psi @16:51:27.68 SS.reconfigure :inhaling 0.95ml/s @16:51:27.72 SS.seek 25ml @16:51:31.13 Stroke completed -- waiting for pressure to equalize ... @16:51:31.14 SS.reconfigure :exhale @16:51:31.18 SS.hold @16:51:36.05 Intake.select :close @16:51:36.19 SSV.dial :exhaust @16:51:36.96 Exhaust.select :open @16:51:37.07 SS.seek 0ml @16:51:42.73 delay 2:00 @16:51:46.16 Exhaust.select :close @16:51:46.27 SSV.dial CBV @16:51:46.31 CBV.dial SSV @16:51:47.05 Allowing 130s for 25.0ml of remaining 800.0ml @16:51:47.07 Intake.select :open @16:51:49.22 Intake @15.7psia, vacuum from 10.0 to 13.0psi @16:51:49.24 SS.reconfigure :inhaling 0.95ml/s @16:51:49.28 SS.seek 25ml @16:52:17.83 Stroke completed -- waiting for pressure to equalize ... @16:52:17.84 SS.reconfigure :exhale @16:52:17.89 SS.hold @16:52:26.37 Intake.select :close @16:52:26.51 SSV.dial :exhaust @16:52:27.27 Exhaust.select :open @16:52:27.38 SS.seek 0ml @16:52:36.41 Exhaust.select :close @16:52:36.52 SSV.dial CBV @16:52:36.55 CBV.dial SSV @16:52:37.29 Allowing 130s for 25.0ml of remaining 775.0ml @16:52:37.30 Intake.select :open @16:52:39.45 Intake @15.8psia, vacuum from 10.0 to 13.0psi @16:52:39.47 SS.reconfigure :inhaling 0.95ml/s @16:52:39.51 SS.seek 25ml @16:53:07.93 Stroke completed -- waiting for pressure to equalize ... @16:53:07.94 SS.reconfigure :exhale @16:53:08.01 Ignoring excess vacuum after stroke's end ... @16:53:08.02 SS.reconfigure :exhale @16:53:08.06 SS.hold @16:53:17.73 Intake.select :close @16:53:17.87 SSV.dial :exhaust @16:53:18.62 Exhaust.select :open @16:53:18.73 SS.seek 0ml @16:53:27.78 Exhaust.select :close @16:53:27.89 SSV.dial CBV @16:53:27.91 CBV.dial SSV @16:53:28.67 Allowing 130s for 25.0ml of remaining 750.0ml @16:53:28.69 Intake.select :open @16:53:30.84 Intake @15.7psia, vacuum from 10.0 to 13.0psi @16:53:30.86 SS.reconfigure :inhaling 0.95ml/s @16:53:30.91 SS.seek 25ml @16:53:42.75 recoverPSRreagent08 @16:53:42.77 PTV.dial :puck @16:53:42.78 PBV.dial :waste1 @16:53:43.13 delay 30 seconds @16:53:55.18 Vacuum too high -- waiting up to 40 seconds @16:53:55.19 SS.reconfigure :exhale @16:53:55.24 SS.hold @16:53:59.44 Intake @14.1psia, vacuum from 8.6 to 11.6psi @16:53:59.46 SS.reconfigure :inhaling 0.87ml/s @16:53:59.50 SS.seek 25ml @16:54:04.31 Stroke completed -- waiting for pressure to equalize ... @16:54:04.32 SS.reconfigure :exhale @16:54:04.37 SS.hold @16:54:10.78 Intake.select :close @16:54:10.92 SSV.dial :exhaust @16:54:11.67 Exhaust.select :open @16:54:11.78 SS.seek 0ml @16:54:13.56 PBV.dial between PSV and waste1 @16:54:13.91 PSR.select :air @16:54:13.93 PSV.dial PSR @16:54:14.70 PS.reconfigure :fast @16:54:14.74 PS.seek 2.5ml @16:54:20.77 Exhaust.select :close @16:54:20.87 SSV.dial CBV @16:54:20.89 CBV.dial SSV @16:54:21.66 Allowing 130s for 25.0ml of remaining 725.0ml @16:54:21.67 Intake.select :open @16:54:23.82 Intake @15.7psia, vacuum from 10.0 to 13.0psi @16:54:23.84 SS.reconfigure :inhaling 0.87ml/s @16:54:23.89 SS.seek 25ml @16:54:38.47 PSV.dial PTV @16:54:39.24 delay 5 seconds @16:54:44.26 PSV.dial PSR @16:54:45.13 delay 5 seconds @16:54:50.15 PSV.dial PTV @16:54:51.01 delay 5 seconds @16:54:54.98 Stroke completed -- waiting for pressure to equalize ... @16:54:55.00 SS.reconfigure :exhale @16:54:55.04 SS.hold @16:54:56.02 PSV.dial :air @16:54:56.79 PS.seek 0ml @16:55:06.09 Intake.select :close @16:55:06.23 SSV.dial :exhaust @16:55:06.97 Exhaust.select :open @16:55:07.08 SS.seek 0ml @16:55:15.93 Exhaust.select :close @16:55:16.04 SSV.dial CBV @16:55:16.06 CBV.dial SSV @16:55:16.81 Allowing 130s for 25.0ml of remaining 700.0ml @16:55:16.83 Intake.select :open @16:55:18.98 Intake @15.7psia, vacuum from 10.0 to 13.0psi @16:55:19.00 SS.reconfigure :inhaling 0.87ml/s @16:55:19.04 SS.seek 25ml @16:55:19.94 washCycleDA #2 of 2 @16:55:19.95 PSR.select :air @16:55:19.97 PSV.dial PSR @16:55:21.33 Pulling 0.5ml of PSR.wash @16:55:21.34 PSR.select :wash @16:55:21.45 PS.reconfigure :default @16:55:21.49 PS.seek 0.5ml @16:55:27.45 delay 5 seconds @16:55:32.46 PSR.select :air @16:55:32.58 PS.seek 2ml @16:55:43.43 Vacuum too high -- waiting up to 40 seconds @16:55:43.44 SS.reconfigure :exhale @16:55:43.48 SS.hold @16:55:47.91 Intake @14.5psia, vacuum from 9.0 to 12.0psi @16:55:47.93 SS.reconfigure :inhaling 0.78ml/s @16:55:47.97 SS.seek 25ml @16:55:48.67 PTV.dial :puck @16:55:48.68 PSV.dial PTV @16:55:49.43 PBV.dial :waste1 @16:55:49.85 PS.seek 1.5ml @16:55:55.75 delay 3 seconds @16:55:56.07 Stroke completed -- waiting for pressure to equalize ... @16:55:56.09 SS.reconfigure :exhale @16:55:56.13 SS.hold @16:55:58.77 PBV.dial between PSV and waste1 @16:55:59.14 PS.seek 0ml @16:56:04.60 Intake.select :close @16:56:04.74 SSV.dial :exhaust @16:56:05.48 Exhaust.select :open @16:56:05.59 SS.seek 0ml @16:56:14.75 Exhaust.select :close @16:56:14.86 SSV.dial CBV @16:56:14.88 CBV.dial SSV @16:56:15.35 delay 2:00 @16:56:15.64 Allowing 130s for 25.0ml of remaining 675.0ml @16:56:15.65 Intake.select :open @16:56:17.81 Intake @15.9psia, vacuum from 10.0 to 13.0psi @16:56:17.83 SS.reconfigure :inhaling 0.78ml/s @16:56:17.87 SS.seek 25ml @16:56:47.61 Vacuum too high -- waiting up to 40 seconds @16:56:47.63 SS.reconfigure :exhale @16:56:47.67 SS.hold @16:56:53.34 Intake @15.0psia, vacuum from 9.5 to 12.5psi @16:56:53.35 SS.reconfigure :inhaling 0.70ml/s @16:56:53.40 SS.seek 25ml @16:56:58.82 Stroke completed -- waiting for pressure to equalize ... @16:56:58.84 SS.reconfigure :exhale @16:56:58.88 SS.hold @16:57:05.29 Intake.select :close @16:57:05.44 SSV.dial :exhaust @16:57:06.18 Exhaust.select :open @16:57:06.29 SS.seek 0ml @16:57:15.07 Exhaust.select :close @16:57:15.18 SSV.dial CBV @16:57:15.20 CBV.dial SSV @16:57:15.95 Allowing 130s for 25.0ml of remaining 650.0ml @16:57:15.96 Intake.select :open @16:57:18.12 Intake @15.8psia, vacuum from 10.0 to 13.0psi @16:57:18.14 SS.reconfigure :inhaling 0.70ml/s @16:57:18.18 SS.seek 25ml @16:57:56.29 Stroke completed -- waiting for pressure to equalize ... @16:57:56.30 SS.reconfigure :exhale @16:57:56.35 SS.hold @16:58:07.98 Intake.select :close @16:58:08.12 SSV.dial :exhaust @16:58:08.87 Exhaust.select :open @16:58:08.98 SS.seek 0ml @16:58:15.37 recoverPSRreagent08 @16:58:15.38 PTV.dial :puck @16:58:15.39 PBV.dial :waste1 @16:58:15.75 delay 30 seconds @16:58:17.85 Exhaust.select :close @16:58:17.96 SSV.dial CBV @16:58:17.98 CBV.dial SSV @16:58:18.75 Allowing 130s for 25.0ml of remaining 625.0ml @16:58:18.76 Intake.select :open @16:58:20.91 Intake @15.8psia, vacuum from 10.0 to 13.0psi @16:58:20.93 SS.reconfigure :inhaling 0.70ml/s @16:58:20.98 SS.seek 25ml @16:58:45.77 PBV.dial between PSV and waste1 @16:58:46.15 PSR.select :air @16:58:46.16 PSV.dial PSR @16:58:46.93 PS.reconfigure :fast @16:58:46.97 PS.seek 2.5ml @16:58:55.74 Vacuum too high -- waiting up to 40 seconds @16:58:55.75 SS.reconfigure :exhale @16:58:55.79 SS.hold @16:59:01.45 Intake @14.9psia, vacuum from 9.4 to 12.4psi @16:59:01.47 SS.reconfigure :inhaling 0.63ml/s @16:59:01.51 SS.seek 25ml @16:59:05.68 Stroke completed -- waiting for pressure to equalize ... @16:59:05.69 SS.reconfigure :exhale @16:59:05.73 SS.hold @16:59:10.43 PSV.dial PTV @16:59:11.17 delay 5 seconds @16:59:13.24 Intake.select :close @16:59:13.38 SSV.dial :exhaust @16:59:14.12 Exhaust.select :open @16:59:14.23 SS.seek 0ml @16:59:16.19 PSV.dial PSR @16:59:16.94 delay 5 seconds @16:59:21.96 PSV.dial PTV @16:59:22.83 delay 5 seconds @16:59:23.04 Exhaust.select :close @16:59:23.15 SSV.dial CBV @16:59:23.18 CBV.dial SSV @16:59:23.93 Allowing 130s for 25.0ml of remaining 600.0ml @16:59:23.95 Intake.select :open @16:59:26.10 Intake @15.8psia, vacuum from 10.0 to 13.0psi @16:59:26.12 SS.reconfigure :inhaling 0.63ml/s @16:59:26.16 SS.seek 25ml @16:59:27.85 PSV.dial :air @16:59:28.74 PS.seek 0ml @16:59:51.69 getPMLReagent08 @16:59:51.70 PRV.dial :air @16:59:52.14 delay 5 seconds @16:59:57.16 PTV.dial :PRVmixing @16:59:58.47 delay 5 seconds @17:00:03.49 PRV.dial :ab2 @17:00:03.94 PTV.dial :PRVmixing @17:00:03.95 PSV.dial :mixloop @17:00:04.39 PS.reconfigure :default @17:00:04.44 PS.seek 0.3ml @17:00:08.34 delay 2 seconds @17:00:08.44 Stroke completed -- waiting for pressure to equalize ... @17:00:08.46 SS.reconfigure :exhale @17:00:08.50 SS.hold @17:00:10.41 PRV.dial :air @17:00:11.07 PS.seek 0.55ml @17:00:14.50 addPMLReagent08 @17:00:14.51 PSV.dial :air @17:00:14.98 PS.seek 2ml @17:00:21.33 Intake.select :close @17:00:21.47 SSV.dial :exhaust @17:00:22.21 Exhaust.select :open @17:00:22.33 SS.seek 0ml @17:00:30.62 PSV.dial :PML @17:00:31.10 PTV.dial :mixing @17:00:31.32 Exhaust.select :close @17:00:31.43 SSV.dial CBV @17:00:31.45 CBV.dial SSV @17:00:31.84 PBV.dial :waste1 @17:00:32.30 PS.seek 1.85ml @17:00:32.33 Allowing 130s for 25.0ml of remaining 575.0ml @17:00:32.36 Intake.select :open @17:00:34.53 Intake @15.7psia, vacuum from 10.0 to 13.0psi @17:00:34.55 SS.reconfigure :inhaling 0.63ml/s @17:00:34.59 SS.seek 25ml @17:00:34.69 PBV.dial between PSV and waste1 @17:00:35.07 PS.seek 0ml @17:00:54.75 delay 2 seconds @17:00:56.77 delay 8:00 @17:01:16.63 Stroke completed -- waiting for pressure to equalize ... @17:01:16.64 SS.reconfigure :exhale @17:01:16.69 SS.hold @17:01:30.95 Intake.select :close @17:01:31.09 SSV.dial :exhaust @17:01:31.84 Exhaust.select :open @17:01:31.94 SS.seek 0ml @17:01:41.18 Exhaust.select :close @17:01:41.28 SSV.dial CBV @17:01:41.30 CBV.dial SSV @17:01:42.05 Allowing 130s for 25.0ml of remaining 550.0ml @17:01:42.07 Intake.select :open @17:01:44.22 Intake @15.7psia, vacuum from 10.0 to 13.0psi @17:01:44.24 SS.reconfigure :inhaling 0.63ml/s @17:01:44.29 SS.seek 25ml @17:02:15.67 Vacuum too high -- waiting up to 40 seconds @17:02:15.68 SS.reconfigure :exhale @17:02:15.73 SS.hold @17:02:21.38 Intake @14.7psia, vacuum from 9.2 to 12.2psi @17:02:21.40 SS.reconfigure :inhaling 0.57ml/s @17:02:21.44 SS.seek 25ml @17:02:32.66 Stroke completed -- waiting for pressure to equalize ... @17:02:32.67 SS.reconfigure :exhale @17:02:32.71 SS.hold @17:02:44.36 Intake.select :close @17:02:44.51 SSV.dial :exhaust @17:02:45.26 Exhaust.select :open @17:02:45.37 SS.seek 0ml @17:02:54.36 Exhaust.select :close @17:02:54.47 SSV.dial CBV @17:02:54.50 CBV.dial SSV @17:02:55.24 Allowing 130s for 25.0ml of remaining 525.0ml @17:02:55.25 Intake.select :open @17:02:57.41 Intake @15.7psia, vacuum from 10.0 to 13.0psi @17:02:57.43 SS.reconfigure :inhaling 0.57ml/s @17:02:57.47 SS.seek 25ml @17:03:39.23 Vacuum too high -- waiting up to 40 seconds @17:03:39.24 SS.reconfigure :exhale @17:03:39.29 SS.hold @17:03:45.90 Intake @15.0psia, vacuum from 9.5 to 12.5psi @17:03:45.92 SS.reconfigure :inhaling 0.51ml/s @17:03:45.96 SS.seek 25ml @17:03:50.92 Stroke completed -- waiting for pressure to equalize ... @17:03:50.94 SS.reconfigure :exhale @17:03:50.98 SS.hold @17:04:00.80 Intake.select :close @17:04:00.94 SSV.dial :exhaust @17:04:01.70 Exhaust.select :open @17:04:01.81 SS.seek 0ml @17:04:10.64 Exhaust.select :close @17:04:10.75 SSV.dial CBV @17:04:10.77 CBV.dial SSV @17:04:11.52 Allowing 130s for 25.0ml of remaining 500.0ml @17:04:11.53 Intake.select :open @17:04:13.69 Intake @15.8psia, vacuum from 10.0 to 13.0psi @17:04:13.71 SS.reconfigure :inhaling 0.51ml/s @17:04:13.75 SS.seek 25ml @17:05:04.94 Stroke completed -- waiting for pressure to equalize ... @17:05:04.95 SS.reconfigure :exhale @17:05:04.99 SS.hold @17:05:21.22 Intake.select :close @17:05:21.36 SSV.dial :exhaust @17:05:22.11 Exhaust.select :open @17:05:22.22 SS.seek 0ml @17:05:31.31 Exhaust.select :close @17:05:31.42 SSV.dial CBV @17:05:31.44 CBV.dial SSV @17:05:32.21 Allowing 130s for 25.0ml of remaining 475.0ml @17:05:32.22 Intake.select :open @17:05:34.37 Intake @15.8psia, vacuum from 10.0 to 13.0psi @17:05:34.39 SS.reconfigure :inhaling 0.51ml/s @17:05:34.43 SS.seek 25ml @17:06:19.48 Vacuum too high -- waiting up to 40 seconds @17:06:19.49 SS.reconfigure :exhale @17:06:20.04 SS.hold @17:06:27.37 Intake @15.3psia, vacuum from 9.8 to 12.8psi @17:06:27.38 SS.reconfigure :inhaling 0.47ml/s @17:06:27.43 SS.seek 25ml @17:06:34.55 Stroke completed -- waiting for pressure to equalize ... @17:06:34.56 SS.reconfigure :exhale @17:06:34.60 SS.hold @17:06:45.65 Intake.select :close @17:06:45.79 SSV.dial :exhaust @17:06:46.55 Exhaust.select :open @17:06:46.66 SS.seek 0ml @17:06:55.48 Exhaust.select :close @17:06:55.59 SSV.dial CBV @17:06:55.61 CBV.dial SSV @17:06:56.38 Allowing 130s for 25.0ml of remaining 450.0ml @17:06:56.39 Intake.select :open @17:06:58.55 Intake @15.9psia, vacuum from 10.0 to 13.0psi @17:06:58.56 SS.reconfigure :inhaling 0.47ml/s @17:06:58.61 SS.seek 25ml @17:07:55.18 Stroke completed -- waiting for pressure to equalize ... @17:07:55.20 SS.reconfigure :exhale @17:07:55.24 SS.hold @17:08:13.57 Intake.select :close @17:08:13.72 SSV.dial :exhaust @17:08:14.47 Exhaust.select :open @17:08:14.58 SS.seek 0ml @17:08:23.67 Exhaust.select :close @17:08:23.78 SSV.dial CBV @17:08:23.80 CBV.dial SSV @17:08:24.56 Allowing 130s for 25.0ml of remaining 425.0ml @17:08:24.58 Intake.select :open @17:08:26.73 Intake @15.8psia, vacuum from 10.0 to 13.0psi @17:08:26.75 SS.reconfigure :inhaling 0.47ml/s @17:08:26.79 SS.seek 25ml @17:08:56.79 recoverPRVreagent08 @17:08:56.80 PTV.dial :mixing @17:08:56.81 PBV.dial :waste1 @17:08:57.18 delay 30 seconds @17:09:14.16 Vacuum too high -- waiting up to 40 seconds @17:09:14.17 SS.reconfigure :exhale @17:09:14.21 SS.hold @17:09:23.29 Intake @15.4psia, vacuum from 9.9 to 12.9psi @17:09:23.31 SS.reconfigure :inhaling 0.43ml/s @17:09:23.35 SS.seek 25ml @17:09:27.19 PBV.dial between PSV and waste1 @17:09:27.57 PSV.dial :air @17:09:28.04 PS.reconfigure :fast @17:09:28.08 PS.seek 2.5ml @17:09:33.39 Stroke completed -- waiting for pressure to equalize ... @17:09:33.40 SS.reconfigure :exhale @17:09:33.44 SS.hold @17:09:47.75 Intake.select :close @17:09:47.89 SSV.dial :exhaust @17:09:48.64 Exhaust.select :open @17:09:48.75 SS.seek 0ml @17:09:51.77 PSV.dial :mixloop @17:09:52.24 delay 2 seconds @17:09:54.25 PSV.dial :air @17:09:54.72 delay 2 seconds @17:09:56.74 PSV.dial :mixloop @17:09:57.21 delay 2 seconds @17:09:57.60 Exhaust.select :close @17:09:57.71 SSV.dial CBV @17:09:57.74 CBV.dial SSV @17:09:58.48 Allowing 130s for 25.0ml of remaining 400.0ml @17:09:58.49 Intake.select :open @17:09:59.22 PSV.dial :air @17:09:59.70 PS.seek 0ml @17:10:00.65 Intake @15.9psia, vacuum from 10.0 to 13.0psi @17:10:00.67 SS.reconfigure :inhaling 0.43ml/s @17:10:00.71 SS.seek 25ml @17:10:22.92 washCycleDA #1 of 3 @17:10:22.93 PSR.select :air @17:10:22.95 PSV.dial PSR @17:10:24.30 Pulling 0.5ml of PSR.wash @17:10:24.31 PSR.select :wash @17:10:24.43 PS.reconfigure :default @17:10:24.47 PS.seek 0.5ml @17:10:30.43 delay 5 seconds @17:10:35.44 PSR.select :air @17:10:35.56 PS.seek 2ml @17:10:51.73 PTV.dial :puck @17:10:52.45 PSV.dial PTV @17:10:53.19 PBV.dial :waste1 @17:10:53.63 PS.seek 1.5ml @17:10:55.41 Vacuum too high -- waiting up to 40 seconds @17:10:55.42 SS.reconfigure :exhale @17:10:55.46 SS.hold @17:10:59.61 delay 3 seconds @17:11:02.62 PBV.dial between PSV and waste1 @17:11:03.01 PS.seek 0ml @17:11:04.03 Intake @15.4psia, vacuum from 9.9 to 12.9psi @17:11:04.04 SS.reconfigure :inhaling 0.38ml/s @17:11:04.09 SS.seek 25ml @17:11:14.20 Stroke completed -- waiting for pressure to equalize ... @17:11:14.21 SS.reconfigure :exhale @17:11:14.25 SS.hold @17:11:19.37 delay 2:00 @17:11:26.90 Intake.select :close @17:11:27.04 SSV.dial :exhaust @17:11:27.79 Exhaust.select :open @17:11:27.90 SS.seek 0ml @17:11:36.96 Exhaust.select :close @17:11:37.07 SSV.dial CBV @17:11:37.09 CBV.dial SSV @17:11:37.86 Allowing 130s for 25.0ml of remaining 375.0ml @17:11:37.87 Intake.select :open @17:11:40.02 Intake @15.8psia, vacuum from 10.0 to 13.0psi @17:11:40.04 SS.reconfigure :inhaling 0.38ml/s @17:11:40.08 SS.seek 25ml @17:12:52.44 Stroke completed -- waiting for pressure to equalize ... @17:12:52.46 SS.reconfigure :exhale @17:12:52.50 SS.hold @17:13:12.79 Intake.select :close @17:13:12.93 SSV.dial :exhaust @17:13:13.68 Exhaust.select :open @17:13:13.79 SS.seek 0ml @17:13:19.38 recoverPSRreagent08 @17:13:19.40 PTV.dial :puck @17:13:19.41 PBV.dial :waste1 @17:13:19.78 delay 30 seconds @17:13:22.62 Exhaust.select :close @17:13:22.73 SSV.dial CBV @17:13:22.74 CBV.dial SSV @17:13:23.51 Allowing 130s for 25.0ml of remaining 350.0ml @17:13:23.52 Intake.select :open @17:13:25.68 Intake @15.8psia, vacuum from 10.0 to 13.0psi @17:13:25.70 SS.reconfigure :inhaling 0.38ml/s @17:13:25.74 SS.seek 25ml @17:13:49.80 PBV.dial between PSV and waste1 @17:13:50.15 PSR.select :air @17:13:50.17 PSV.dial PSR @17:13:50.93 PS.reconfigure :fast @17:13:50.97 PS.seek 2.5ml @17:14:14.67 PSV.dial PTV @17:14:15.42 delay 5 seconds @17:14:20.44 PSV.dial PSR @17:14:21.19 delay 5 seconds @17:14:26.21 PSV.dial PTV @17:14:26.95 delay 5 seconds @17:14:31.97 PSV.dial :air @17:14:32.72 PS.seek 0ml @17:14:39.52 Stroke completed -- waiting for pressure to equalize ... @17:14:39.53 SS.reconfigure :exhale @17:14:39.57 SS.hold @17:14:55.82 washCycleDA #2 of 3 @17:14:55.83 PSR.select :air @17:14:55.85 PSV.dial PSR @17:14:57.21 Pulling 0.5ml of PSR.wash @17:14:57.22 PSR.select :wash @17:14:57.33 PS.reconfigure :default @17:14:57.37 PS.seek 0.5ml @17:15:01.91 Intake.select :close @17:15:02.05 SSV.dial :exhaust @17:15:02.80 Exhaust.select :open @17:15:03.39 delay 5 seconds @17:15:03.44 SS.seek 0ml @17:15:08.40 PSR.select :air @17:15:08.51 PS.seek 2ml @17:15:12.25 Exhaust.select :close @17:15:12.36 SSV.dial CBV @17:15:12.39 CBV.dial SSV @17:15:13.13 Allowing 130s for 25.0ml of remaining 325.0ml @17:15:13.14 Intake.select :open @17:15:15.30 Intake @15.9psia, vacuum from 10.0 to 13.0psi @17:15:15.32 SS.reconfigure :inhaling 0.38ml/s @17:15:15.36 SS.seek 25ml @17:15:24.73 PTV.dial :puck @17:15:24.74 PSV.dial PTV @17:15:25.46 PBV.dial :waste1 @17:15:25.88 PS.seek 1.5ml @17:15:31.82 delay 3 seconds @17:15:34.84 PBV.dial between PSV and waste1 @17:15:35.21 PS.seek 0ml @17:15:51.36 delay 2:00 @17:16:17.22 Vacuum too high -- waiting up to 40 seconds @17:16:17.23 SS.reconfigure :exhale @17:16:17.27 SS.hold @17:16:27.82 Intake @15.6psia, vacuum from 10.0 to 13.0psi @17:16:27.84 SS.reconfigure :inhaling 0.34ml/s @17:16:27.88 SS.seek 25ml @17:16:42.05 Stroke completed -- waiting for pressure to equalize ... @17:16:42.06 SS.reconfigure :exhale @17:16:42.10 SS.hold @17:17:00.44 Intake.select :close @17:17:00.59 SSV.dial :exhaust @17:17:01.33 Exhaust.select :open @17:17:01.44 SS.seek 0ml @17:17:10.25 Exhaust.select :close @17:17:10.36 SSV.dial CBV @17:17:10.38 CBV.dial SSV @17:17:11.14 Allowing 130s for 25.0ml of remaining 300.0ml @17:17:11.16 Intake.select :open @17:17:13.31 Intake @15.9psia, vacuum from 10.0 to 13.0psi @17:17:13.33 SS.reconfigure :inhaling 0.34ml/s @17:17:13.37 SS.seek 25ml @17:17:51.38 recoverPSRreagent08 @17:17:51.39 PTV.dial :puck @17:17:51.40 PBV.dial :waste1 @17:17:51.76 delay 30 seconds @17:18:21.78 PBV.dial between PSV and waste1 @17:18:22.13 PSR.select :air @17:18:22.15 PSV.dial PSR @17:18:22.90 PS.reconfigure :fast @17:18:22.95 PS.seek 2.5ml @17:18:39.98 Stroke completed -- waiting for pressure to equalize ... @17:18:40.00 SS.reconfigure :exhale @17:18:40.04 SS.hold @17:18:46.69 PSV.dial PTV @17:18:47.43 delay 5 seconds @17:18:52.44 PSV.dial PSR @17:18:53.19 delay 5 seconds @17:18:58.21 PSV.dial PTV @17:18:58.96 delay 5 seconds @17:19:03.97 PSV.dial :air @17:19:04.73 PS.seek 0ml @17:19:06.98 Intake.select :close @17:19:07.12 SSV.dial :exhaust @17:19:07.88 Exhaust.select :open @17:19:07.99 SS.seek 0ml @17:19:16.83 Exhaust.select :close @17:19:16.93 SSV.dial CBV @17:19:16.95 CBV.dial SSV @17:19:17.70 Allowing 130s for 25.0ml of remaining 275.0ml @17:19:17.72 Intake.select :open @17:19:19.87 Intake @15.8psia, vacuum from 10.0 to 13.0psi @17:19:19.89 SS.reconfigure :inhaling 0.34ml/s @17:19:19.94 SS.seek 25ml @17:19:27.98 washCycleDA #3 of 3 PSR.select :air @17:19:28.00 PSV.dial PSR @17:19:29.36 Pulling 0.5ml of PSR.wash @17:19:29.37 PSR.select :wash @17:19:29.48 PS.reconfigure :default @17:19:29.53 PS.seek 0.5ml @17:19:35.41 delay 5 seconds @17:19:40.43 PSR.select :air @17:19:40.54 PS.seek 2ml @17:19:56.72 PTV.dial :puck @17:19:56.74 PSV.dial PTV @17:19:57.58 PBV.dial :waste1 @17:19:58.02 PS.seek 1.5ml @17:20:03.91 delay 3 seconds @17:20:06.93 PBV.dial between PSV and waste1 @17:20:07.31 PS.seek 0ml @17:20:23.66 delay 2:00 @17:20:40.30 Vacuum too high -- waiting up to 40 seconds @17:20:40.32 SS.reconfigure :exhale @17:20:40.36 SS.hold @17:20:54.76 Intake @15.6psia, vacuum from 10.0 to 13.0psi @17:20:54.78 SS.reconfigure :inhaling 0.31ml/s @17:20:54.82 SS.seek 25ml @17:21:02.20 Stroke completed -- waiting for pressure to equalize ... @17:21:02.21 SS.reconfigure :exhale @17:21:02.25 SS.hold @17:21:18.47 Intake.select :close @17:21:18.61 SSV.dial :exhaust @17:21:19.34 Exhaust.select :open @17:21:19.45 SS.seek 0ml @17:21:28.21 Exhaust.select :close @17:21:28.32 SSV.dial CBV @17:21:28.34 CBV.dial SSV @17:21:29.12 Allowing 130s for 25.0ml of remaining 250.0ml @17:21:29.14 Intake.select :open @17:21:31.29 Intake @15.9psia, vacuum from 10.0 to 13.0psi @17:21:31.31 SS.reconfigure :inhaling 0.31ml/s @17:21:31.88 SS.seek 25ml @17:22:23.69 recoverPSRreagent08 @17:22:23.70 PTV.dial :puck @17:22:23.71 PBV.dial :waste1 @17:22:24.08 delay 30 seconds @17:22:51.24 Vacuum too high -- waiting up to 40 seconds @17:22:51.25 SS.reconfigure :exhale @17:22:51.29 SS.hold @17:22:54.10 PBV.dial between PSV and waste1 @17:22:54.47 PSR.select :air @17:22:54.49 PSV.dial PSR @17:22:55.24 PS.reconfigure :fast @17:22:55.28 PS.seek 2.5ml @17:23:02.44 Intake @15.6psia, vacuum from 10.0 to 13.0psi @17:23:02.46 SS.reconfigure :inhaling 0.28ml/s @17:23:02.50 SS.seek 25ml @17:23:19.00 PSV.dial PTV @17:23:19.88 delay 5 seconds @17:23:20.76 Stroke completed -- waiting for pressure to equalize ... @17:23:20.77 SS.reconfigure :exhale @17:23:20.81 SS.hold @17:23:24.89 PSV.dial PSR @17:23:25.63 delay 5 seconds @17:23:30.64 PSV.dial PTV @17:23:31.39 delay 5 seconds @17:23:36.41 PSV.dial :air @17:23:37.16 PS.seek 0ml @17:23:39.15 SS.reconfigure :default @17:23:39.20 SS.hold @17:23:39.32 Intake.select :close @17:23:39.46 ShallowSampler::Clogged in WCR -- Filtering too Slowly after 775.0ml @17:23:39.48 SSV.dial :exhaust @17:23:40.21 Exhaust.select :open @17:23:40.32 SS.reconfigure :exhale @17:23:40.37 SS.seek 0ml @17:23:49.18 Exhaust.select :close @17:23:49.29 Sampled 775.0ml @17:23:49.31 Drying puck before fixing for 20:00 @17:23:49.32 CBV.dial :waste2b @17:23:50.06 delay 1:00 @17:24:00.50 Gate.power :camera,:ON @17:24:00.51 Mixing DA Substrates @17:24:00.52 PSR.select :air @17:24:00.54 PSV.dial PSR @17:24:02.05 Pulling 0.25ml of PSR.sub1 @17:24:02.10 PSR.select :sub1 @17:24:02.28 PS.reconfigure :default @17:24:02.39 PS.seek 0.25ml @17:24:05.82 delay 5 seconds @17:24:10.90 Pulling 0.25ml of PSR.sub2 @17:24:10.91 PSR.select :sub2 @17:24:11.02 PS.seek 0.5ml @17:24:14.38 delay 5 seconds @17:24:19.40 PSR.select :air @17:24:19.50 PS.seek 2ml @17:24:35.72 PTV.dial :puck @17:24:35.73 PSV.dial PTV @17:24:36.47 PBV.dial :waste1 @17:24:36.90 PS.seek 1.5ml @17:24:42.91 delay 3 seconds @17:24:45.92 PBV.dial between PSV and waste1 @17:24:46.30 PS.seek 0ml @17:24:50.07 CSV.dial :air @17:24:50.52 CS.reconfigure :fast @17:24:50.57 CS.seek 10ml @17:25:02.44 delay 10 seconds @17:25:12.46 recoverPSRreagent08 @17:25:12.47 PTV.dial :puck @17:25:12.48 PBV.dial :waste1 @17:25:12.84 delay 30 seconds @17:25:15.26 Pumping wcr puck from tube 2 up to 35psi... @17:25:15.27 CBV.dial SSV @17:25:17.10 CBV.dial between CSV and waste2b @17:25:18.05 CSV.dial between air and CTV @17:25:18.38 CS.seek 7.23ml @17:25:25.64 CTV.dial CSV,avoiding: :air @17:25:25.66 CSV.dial CTV @17:25:26.49 CS.reconfigure :pressurize @17:25:26.54 CS.seek 0ml @17:25:42.86 PBV.dial between PSV and waste1 @17:25:43.23 PSR.select :air @17:25:43.25 PSV.dial PSR @17:25:44.02 PS.reconfigure :fast @17:25:44.06 PS.seek 2.5ml @17:25:44.77 CBV.dial :waste2b @17:25:45.10 CS.reconfigure :default @17:25:45.15 CS.seek 0ml @17:25:46.60 delay 1:00 @17:26:07.96 PSV.dial PTV @17:26:08.73 delay 5 seconds @17:26:13.75 PSV.dial PSR @17:26:14.51 delay 5 seconds @17:26:19.53 PSV.dial PTV @17:26:20.42 delay 5 seconds @17:26:25.44 PSV.dial :air @17:26:26.22 PS.seek 0ml @17:26:46.62 CTV.dial :air,avoiding: :intake @17:26:47.95 Fix #1 of 2 (with RNAlater) for 10:00 @17:26:47.96 CSV.dial CSR @17:26:48.46 Pulling 2ml of CSR.RNAlater @17:26:48.48 CSR.select :RNAlater @17:26:48.59 CS.seek 2ml @17:26:49.50 Additional air clear @17:26:49.51 PSR.select :air @17:26:49.52 PSV.dial PSR @17:26:50.89 Pulling 2ml of PSR.air @17:26:50.90 PSR.select :air @17:26:50.93 PS.reconfigure :default @17:26:50.97 PS.seek 2ml @17:26:54.60 delay 5 seconds @17:26:59.62 CSR.select :air @17:26:59.73 CS.reconfigure :fast @17:26:59.77 CS.seek 10ml @17:27:12.18 delay 5 seconds @17:27:17.20 PTV.dial :puck @17:27:17.22 PSV.dial PTV @17:27:17.96 PBV.dial :waste1 @17:27:18.40 PS.seek 1.5ml @17:27:19.57 Pumping wcr puck from tube 2 up to 35psi... @17:27:19.58 CBV.dial SSV @17:27:20.88 CBV.dial between CSV and waste2b @17:27:21.84 CSV.dial between air and CTV @17:27:22.45 CS.seek 7.147ml @17:27:24.40 delay 3 seconds @17:27:27.42 PBV.dial between PSV and waste1 @17:27:27.80 delay 1 second @17:27:28.81 PSV.dial :waste1 @17:27:29.76 CTV.dial CSV,avoiding: :air @17:27:29.78 CSV.dial CTV @17:27:29.91 PS.seek 0ml @17:27:31.15 CS.reconfigure :pressurize @17:27:31.20 CS.seek 0ml @17:27:46.08 Processing Puck Heater at 28C before imaging @17:27:46.09 PH.coast @17:27:46.21 Imaging da puck in Processing Clamp while storing it to tube 1 @17:27:46.37 SC.reconfigure :tight @17:27:46.42 SC.seek 1 @17:27:49.52 delay 10:00 @17:27:50.05 SC.configure :limp @17:27:50.25 Elbow.reconfigure :tight @17:27:50.29 Elbow.seek 1 @17:27:51.96 Forearm.reconfigure :tight @17:27:52.01 Forearm.seek 1 + 450 counts @17:27:53.07 SE.reconfigure :pick @17:27:53.12 SE.seek :beamBreak @17:27:55.10 SE.configure :default @17:27:55.24 Elbow.reconfigure :limp @17:27:55.29 Forearm.reconfigure :loose @17:27:55.33 Forearm.seek 1 @17:27:55.90 Elbow.hold @17:27:55.96 Elbow.reconfigure :tight @17:27:56.05 SE.seek 17.08 Pucks below Top Plate @17:27:56.52 Forearm.force -20 @17:27:56.55 Forearm.seek 1 + 298 counts @17:27:56.84 Forearm.reconfigure :tight @17:27:56.89 Forearm.seek 1 + 450 counts @17:27:57.10 SE.seek 18.08 Pucks below Top Plate @17:27:57.74 Forearm.reconfigure :loose @17:27:57.78 Forearm.seek :retract @17:27:58.72 Elbow.seek PC @17:28:01.22 Elbow.reconfigure :loose @17:28:01.31 Forearm.seek PC @17:28:02.31 Hand.close @17:28:02.95 PC.seek :open @17:28:07.72 Forearm.seek :retract @17:28:08.67 Elbow.reconfigure :tight @17:28:08.71 Elbow.seek 1 @17:28:11.42 Forearm.reconfigure :tight @17:28:11.46 Forearm.seek 1 + 450 counts @17:28:12.40 Hand.open @17:28:13.01 SE.seek 17.73 Pucks below Top Plate @17:28:13.27 SE.reconfigure :seal @17:28:13.32 SE.seek 16.77 Pucks below Top Plate @17:28:13.85 Lights, camera, ACTION! @17:28:13.88 PuckCamera: snap -bin=2,2 -auto /var/log/esp/da22nov2917h850ml.tif @17:28:32.27 SX HX9/16 (USB:05): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15.101 seconds /var/log/esp/da22nov2917h850ml.tif: TIFF Upload Complete @17:28:32.29 PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da22nov2917h850ml15s.tif @17:28:33.37 Waiting >20 seconds for da to relinquish FlushPuck @17:28:47.91 SX HX9/16 (USB:05): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15 seconds /var/log/esp/midres/da22nov2917h850ml15s.tif: TIFF Upload Complete @17:28:47.93 PuckCamera: snap -bin=2,2 20.0 /var/log/esp/midres/da22nov2917h850ml20s.tif @17:29:08.59 SX HX9/16 (USB:05): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 20 seconds /var/log/esp/midres/da22nov2917h850ml20s.tif: TIFF Upload Complete @17:29:08.60 Gate.power :camera,:OFF @17:29:08.62 DAfans.email "15.101 sec auto-exposure",Subject: "da22nov2917h850ml images!" @17:29:08.89 Elbow.seek 1 @17:29:09.75 Forearm.seek 1 + 450 counts @17:29:09.83 SE.reconfigure :gently @17:29:09.88 SE.seek 17.48 Pucks below Top Plate @17:29:10.92 Elbow.reconfigure :limp @17:29:10.98 Forearm.reconfigure :loose @17:29:11.03 Forearm.seek 1 @17:29:11.31 Elbow.hold @17:29:11.38 Elbow.reconfigure :tight @17:29:11.43 SE.reconfigure :default @17:29:11.47 SE.seek 17.67 Pucks below Top Plate @17:29:11.90 Forearm.force -20 @17:29:11.94 Forearm.seek 1 + 306 counts @17:29:12.26 Forearm.reconfigure :tight @17:29:12.30 Forearm.seek 1 + 450 counts @17:29:12.63 SE.seek :down @17:29:13.76 Forearm.reconfigure :loose @17:29:13.81 Forearm.seek :retract @17:29:14.11 SE.stop @17:29:14.91 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @17:29:15.28 Elbow.seek FlushPuck::Garage @17:29:17.25 Elbow.reconfigure :loose @17:29:17.44 Forearm.seek :garage @17:29:18.50 Hand.close @17:29:19.45 Forearm.seek :retract @17:29:20.47 Elbow.reconfigure :tight @17:29:21.66 Elbow.seek PC @17:29:23.00 Elbow.reconfigure :loose @17:29:23.20 Forearm.reconfigure :tight @17:29:23.29 Forearm.seek PC @17:29:24.93 PC.seek :closed @17:29:29.67 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.59 @17:29:29.82 Hand.open @17:29:30.62 Forearm.reconfigure :loose @17:29:30.72 Forearm.seek :retract @17:29:31.73 PSR.select :air @17:29:31.77 PSV.dial PSR @17:29:32.38 Pulling 0.5ml of PSR.flush @17:29:32.40 PSR.select :flush @17:29:32.57 PS.seek 0.5ml @17:29:38.53 delay 5 seconds @17:29:43.39 upsync @17:29:43.55 PSR.select :air @17:29:43.67 PS.seek 2.5ml @17:30:04.93 delay 1 second @17:30:05.95 PRV.dial :waste2,avoiding: :ab2 @17:30:07.80 PTV.dial :PRVmixing @17:30:09.10 PSV.dial :mixloop @17:30:10.14 PS.seek :empty @17:30:36.52 PSR.select :air @17:30:36.54 PSV.dial PSR @17:30:37.65 Pulling 0.5ml of PSR.flush @17:30:37.66 PSR.select :flush @17:30:37.78 PS.seek 0.5ml @17:30:43.78 delay 5 seconds @17:30:48.79 PSR.select :air @17:30:48.91 PS.seek 2.5ml @17:31:10.21 delay 1 second @17:31:11.23 PBV.dial :waste1 @17:31:11.60 PTV.dial :mixing @17:31:12.33 PSV.dial :mixloop @17:31:13.38 PS.seek :empty @17:31:39.71 PSV.dial PSR @17:31:40.75 PSR.select :flush @17:31:40.87 PS.seek 1ml @17:31:51.92 PSR.select :air @17:31:52.04 PS.seek 2.5ml @17:32:08.21 PSV.dial PTV @17:32:08.96 PTV.dial :puck @17:32:09.69 PBV.dial :waste1 @17:32:09.71 PS.seek 0ml @17:32:36.08 PSV.dial PSR @17:32:36.83 PSR.select :flush @17:32:36.95 PS.seek 0.5ml @17:32:42.95 PSR.select :air @17:32:43.07 PS.seek 2.5ml @17:33:04.30 PSV.dial PTV @17:33:05.05 PBV.dial PSV @17:33:05.62 PS.seek 0ml @17:33:31.92 PSV.dial PBV @17:33:32.40 PS.seek 2.5ml @17:33:58.77 PSV.dial :waste1 @17:33:59.51 PS.seek 0ml @17:34:25.95 PSV.dial PSR @17:34:26.42 PSR.select :air @17:34:26.45 PS.seek 2.5ml @17:34:52.73 PSV.dial PTV @17:34:53.47 PBV.dial PSV @17:34:53.49 PS.seek 0ml @17:35:20.01 PSV.dial PBV @17:35:20.49 PS.seek 2.5ml @17:35:46.86 PSV.dial :waste1 @17:35:47.60 PS.seek 0ml @17:36:13.98 PSV.dial PSR @17:36:14.45 PSR.select :flush @17:36:14.57 PS.seek 1ml @17:36:25.64 PSR.select :air @17:36:25.75 PS.seek 2.5ml @17:36:41.89 PSV.dial PTV @17:36:42.63 PTV.dial :puck @17:36:42.64 PBV.dial :waste1 @17:36:43.21 PS.seek 0ml @17:37:09.57 PSV.dial PSR @17:37:10.33 PSR.select :flush @17:37:10.44 PS.seek 0.5ml @17:37:16.82 PSR.select :air @17:37:16.94 PS.seek 2.5ml @17:37:38.23 PSV.dial PTV @17:37:39.11 PBV.dial PSV @17:37:39.68 PS.seek 0ml @17:37:49.55 CBV.dial :waste2b @17:37:49.87 CS.reconfigure :default @17:37:49.92 CS.seek 0ml @17:37:50.96 delay 1:00 @17:38:06.04 PSV.dial PBV @17:38:06.50 PS.seek 2.5ml @17:38:32.89 PSV.dial :waste1 @17:38:33.63 PS.seek 0ml @17:38:50.98 CTV.dial :air,avoiding: :intake @17:38:52.31 Fix #2 of 2 (with RNAlater) for 10:00 @17:38:52.32 CSV.dial CSR @17:38:52.81 Pulling 2ml of CSR.RNAlater @17:38:52.82 CSR.select :RNAlater @17:38:52.93 CS.seek 2ml @17:38:58.86 delay 5 seconds @17:38:59.92 PSV.dial PSR @17:39:00.39 PSR.select :air @17:39:00.41 PS.seek 2.5ml @17:39:03.88 CSR.select :air @17:39:04.00 CS.reconfigure :fast @17:39:04.04 CS.seek 10ml @17:39:23.55 Pumping wcr puck from tube 2 up to 35psi... @17:39:23.57 CBV.dial SSV @17:39:24.85 CBV.dial between CSV and waste2b @17:39:25.80 CSV.dial between air and CTV @17:39:26.40 CS.seek 7.123ml @17:39:26.76 PSV.dial PTV @17:39:27.50 PBV.dial PSV @17:39:27.52 PS.seek 0ml @17:39:33.90 CTV.dial CSV,avoiding: :air @17:39:33.92 CSV.dial CTV @17:39:35.31 CS.reconfigure :pressurize @17:39:35.35 CS.seek 0ml @17:39:52.35 delay 10:00 @17:39:53.85 PSV.dial PBV @17:39:54.33 PS.seek 2.5ml @17:40:20.63 PSV.dial :waste1 @17:40:21.38 PS.seek 0ml @17:40:47.76 Garaging FlushPuck in Processing Clamp @17:40:47.83 Elbow.reconfigure :tight @17:40:47.88 Elbow.seek PC @17:40:47.99 Elbow.reconfigure :loose @17:40:48.08 Forearm.seek PC @17:40:49.01 Hand.close @17:40:49.56 PC.seek :open @17:40:54.28 Forearm.seek :retract @17:40:55.28 Elbow.reconfigure :tight @17:40:55.32 Elbow.seek :garage @17:40:56.68 Elbow.reconfigure :loose @17:40:56.77 Forearm.reconfigure :tight @17:40:56.82 Forearm.seek :garage @17:40:57.84 Hand.open @17:40:58.49 Forearm.reconfigure :loose @17:40:58.54 Forearm.seek :retract @17:49:52.38 CBV.dial :waste2b @17:49:52.70 CS.reconfigure :default @17:49:52.74 CS.seek 0ml @17:49:55.38 delay 1:00 @17:50:55.41 CTV.dial :air,avoiding: :intake @17:50:56.77 Storing wcr puck in Collection Clamp to tube 1 @17:50:56.93 SC.reconfigure :tight @17:50:56.97 SC.seek 1 @17:50:57.08 SC.configure :limp @17:50:57.27 Elbow.reconfigure :tight @17:50:57.32 Elbow.seek 1 @17:50:59.17 Forearm.reconfigure :tight @17:50:59.68 Forearm.seek 1 + 450 counts @17:51:00.73 SE.reconfigure :pick @17:51:00.78 SE.seek :beamBreak @17:51:02.75 SE.configure :default @17:51:02.90 Elbow.reconfigure :limp @17:51:02.95 Forearm.reconfigure :loose @17:51:02.99 Forearm.seek 1 @17:51:03.21 Elbow.hold @17:51:03.27 Elbow.reconfigure :tight @17:51:03.37 SE.seek 18.1 Pucks below Top Plate @17:51:03.95 Forearm.force -20 @17:51:03.99 Forearm.seek 1 + 318 counts @17:51:04.30 Forearm.reconfigure :tight @17:51:04.34 Forearm.seek 1 + 450 counts @17:51:04.57 SE.seek 19.1 Pucks below Top Plate @17:51:05.14 Forearm.reconfigure :loose @17:51:05.18 Forearm.seek :retract @17:51:06.10 Elbow.seek CC @17:51:07.75 Elbow.reconfigure :loose @17:51:07.84 Forearm.seek CC @17:51:08.80 Hand.close @17:51:09.43 CC.seek :open @17:51:14.19 Forearm.seek :retract @17:51:15.15 Elbow.reconfigure :tight @17:51:15.19 Elbow.seek 1 @17:51:17.02 Elbow.reconfigure :limp @17:51:17.07 Forearm.seek 1 @17:51:18.10 Elbow.hold @17:51:18.16 Elbow.reconfigure :tight @17:51:18.21 Hand.open @17:51:18.91 Forearm.force -20 @17:51:18.95 Forearm.seek 1 + 310 counts @17:51:19.25 Forearm.reconfigure :tight @17:51:19.29 Forearm.seek 1 + 450 counts @17:51:19.57 SE.seek :down @17:51:20.70 Forearm.reconfigure :loose @17:51:20.74 Forearm.seek :retract @17:51:20.98 SE.stop @17:51:21.74
Starting finalPurge @17:51:21.75 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @17:51:21.94 Elbow.seek FlushPuck::Garage @17:51:23.67 Elbow.reconfigure :loose @17:51:23.76 Forearm.seek :garage @17:51:24.72 Hand.close @17:51:25.41 Forearm.seek :retract @17:51:26.46 Elbow.reconfigure :tight @17:51:26.50 Elbow.seek CC @17:51:29.42 Elbow.reconfigure :loose @17:51:29.50 Forearm.reconfigure :tight @17:51:29.54 Forearm.seek CC @17:51:30.69 CC.seek :closed @17:51:35.27 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.19 @17:51:35.34 Hand.open @17:51:36.80 Forearm.reconfigure :loose @17:51:36.92 Forearm.seek :retract @17:51:37.90 Flushing the Collection Valve::Manifold @17:51:38.01 CSV.dial CSR @17:51:38.52 Pulling 5ml of CSR.flush CSR.select :flush @17:51:38.64 CS.seek 5ml @17:51:52.19 delay 5 seconds @17:51:57.21 CSR.select :air @17:51:57.36 CS.reconfigure :fast @17:51:57.41 CS.seek 7.5ml @17:52:04.41 CSV.dial :waste @17:52:05.67 CS.reconfigure :default @17:52:05.71 CS.seek 0ml @17:52:25.76 Flushing lower manifold's waste1 port @17:52:25.87 CSV.dial :flush @17:52:26.43 CS.reconfigure :fast @17:52:26.47 CS.seek 5ml @17:52:39.13 delay 5 seconds @17:52:44.15 CSV.dial :air @17:52:44.66 CS.seek 7ml @17:52:50.22 CSV.dial CTV @17:52:50.25
CTV.dial CSV @17:52:51.61 CBV.dial :waste1 @17:52:52.37 CS.seek 0ml @17:53:08.91 Flushing lower manifold's waste2 port @17:53:09.02 CSV.dial :flush @17:53:09.74 CS.seek 5ml @17:53:22.53 delay 5 seconds @17:53:27.55 CSV.dial :air @17:53:28.06 CS.seek 7ml @17:53:33.60 CSV.dial CTV @17:53:33.62
CTV.dial CSV @17:53:34.07 CBV.dial :waste2 @17:53:35.10 CS.seek 0ml @17:53:51.53 Flushing lower manifold's waste2b port @17:53:51.65 CSV.dial :flush @17:53:52.39 CS.seek 5ml @17:54:05.13 delay 5 seconds @17:54:10.15 CSV.dial :air @17:54:10.66 CS.seek 7ml @17:54:16.19 CSV.dial CTV @17:54:16.21
CTV.dial CSV @17:54:16.66 CBV.dial :waste2b @17:54:17.70 CS.seek 0ml @17:54:34.13 Disinfecting external Intake with kill @17:54:34.24 CSV.dial CSR @17:54:34.73 Pulling 8ml of CSR.kill @17:54:34.74 CSR.select :kill @17:54:34.85 CS.seek 8ml @17:54:54.54 delay 5 seconds @17:54:59.55 CSR.select :air @17:54:59.70 CS.seek 10ml @17:55:05.17 CSV.dial CBV @17:55:05.19
CBV.dial CSV @17:55:06.22 CTV.dial :intake @17:55:06.98 Intake.select :open @17:55:07.09 CS.seek 2ml @17:55:25.75 Intake.select :close @17:55:25.85 CTV.dial :air @17:55:26.60 CS.reconfigure :default @17:55:26.64 CS.seek 0ml @17:55:32.53 CSV.dial CSR @17:55:33.57 Pulling 5ml of CSR.flush @17:55:33.58 CSR.select :flush @17:55:33.69 CS.seek 5ml @17:55:47.47 delay 5 seconds @17:55:52.49 CSR.select :air @17:55:52.60 CS.seek 10ml @17:56:06.28 CSV.dial CBV @17:56:07.27 CS.seek 0ml @17:56:33.66 Garaging FlushPuck in Collection Clamp @17:56:33.72 Elbow.reconfigure :tight @17:56:33.77 Elbow.seek CC @17:56:34.10 Elbow.reconfigure :loose @17:56:34.19 Forearm.seek CC @17:56:35.15 Hand.close @17:56:35.80 CC.seek :open @17:56:40.45 Forearm.seek :retract @17:56:41.41 Elbow.reconfigure :tight @17:56:41.45 Elbow.seek :garage @17:56:44.34 Elbow.reconfigure :loose @17:56:44.42 Forearm.reconfigure :tight @17:56:44.46 Forearm.seek :garage @17:56:45.50 Hand.open @17:56:46.10 Forearm.reconfigure :loose @17:56:46.15 Forearm.seek :retract @17:56:47.13 Completed [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] begun 15:24:42 @17:56:47.17 Asleep after completing DA_WCR_112922 assays @17:56:47.19 GoodNews.email "{1 => 20,\n 2 => 13,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@17:53:52, 17% flow, 22.8C, 23% humidity, 15.0psia, 13.720V, 0.550A, 0.532A avg, 7.55W\nSampled 775.0ml",Subject: "Asleep after completing DA_WCR_112922 assays" @17:56:54.14 upsync @13:37:00.12UTC06-Dec-22
ESP2 v3.41 I2C Gateway v3.20 for core bus @13:37:02.69 Configuration for short puck jake 1/11/21 by brent@mbari.org /home/esp/esp2/type/shallow/jake/configure.rb @13:37:06.64 Polling Can every 10:00 @13:37:06.69 Cleanly exited at 13:34:37UTC06-Dec-22 @13:37:06.73 Loading /home/esp/esp2/mission/DA_WCR_120622.rb mission @13:37:06.78 GoodNews.email "Starting DA_WCR_120622 w/pucks in tube 2\n{1 => 20,\n 2 => 13,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@13:37:06, 16% flow, 22.4C, 26% humidity, 15.0psia, 13.773V, 0.327A, 0.315A avg, 4.50W",Subject: "DA_WCR_120622 begun" @13:37:06.82
Preparing to sleep until + 00:00:05 @13:37:06.85 Asleep til + 00:00:05 @13:37:06.86 Continuing at 13:37 @13:37:07.15 Gate.power :core,:OFF @13:37:07.16 Gate.power :raw,:OFF @13:37:07.17 Shutdown @13:37:11.31 GoodNews.email "Then, in tube 2: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2/mission/DA_WCR_120622.rb:14\n{1 => 20,\n 2 => 13,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@13:37:06, 16% flow, 22.4C, 26% humidity, 15.0psia, 13.773V, 0.327A, 0.315A avg, 4.50W",Subject: "Asleep until 13:37" @13:37:11.75
Powering core bus @13:37:13.02 upsync @13:37:16.86 upsync @13:37:16.97
Gate.power :raw,:ON @13:37:17.92 CS.configure :default @13:37:18.01 PS.configure :default @13:37:18.10 SS.configure :default @13:37:18.19 CC.configure :default @13:37:18.28 PC.configure :default @13:37:18.39 Forearm.configure :loose @13:37:18.47 Elbow.configure :loose @13:37:18.58 SC.configure :loose @13:37:18.67 SE.configure :default @13:37:18.71 CH.coast @13:37:18.76 CH.configure :default @13:37:18.79 PH.coast @13:37:18.85 PH.configure :default @13:37:18.88 All dwarf controllers running firmware version 3.85 @13:37:18.98 Valve.reset @13:37:19.19 Intake.select :close @13:37:19.26 Exhaust.select :close @13:37:19.29 CTV.dial :air @13:37:19.39 PSV.dial :air @13:37:19.47 CSV.dial :waste1 @13:37:19.49
Forearm.reconfigure :loose @13:37:19.57 Forearm.home.seek :retract @13:37:19.94 SSV.dial :spit,avoiding: :exhaust @13:37:20.32 Hand.close @13:37:20.36
Elbow.jog 1500 @13:37:20.57 PS.reconfigure :default @13:37:20.61 PS.home.seek 0ml @13:37:20.74 CS.reconfigure :fast @13:37:20.77 SS.reconfigure :default @13:37:20.86 CS.home.seek 0ml @13:37:20.89 SS.home.seek 0ml @13:37:21.01
Elbow.reconfigure :loose @13:37:21.05 Elbow.home.seek :clear @13:37:25.51 SE.reconfigure :default @13:37:25.55 SE.home.seek :down @13:37:26.22 SC.reconfigure :tight @13:37:26.26 SC.home.seek :initial @13:37:28.90 PS.stop @13:37:54.60
SC.configure :limp @13:37:54.65 Forearm.seek :retract @13:37:54.80 Elbow.seek :hammer @13:37:55.82 Forearm.reconfigure :tight @13:37:55.86 Forearm.seek retracted @13:37:56.17 PC.reconfigure :default @13:37:56.19
CC.reconfigure :default @13:37:56.28 PC.home.seek :open @13:37:56.30
CC.home.seek :open @13:37:59.77 Phase [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] @13:37:59.78 Notice.email "At 13:37:59 began in tube 2: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2/mission/DA_WCR_120622.rb:14\n{1 => 20,\n 2 => 13,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@13:37:59, 15% flow, 22.3C, 26% humidity, 15.0psia, 13.783V, 0.272A, 0.260A avg, 3.75W",Subject: "Started DA_WCR_120622 phase initialPurge; da [1000, 1000] #da and wsr are run simultanelously" @13:37:59.81
Starting initialPurge @13:37:59.83 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @13:37:59.90 Hand.open @13:38:00.69 Elbow.reconfigure :tight @13:38:00.73 Elbow.seek FlushPuck::Garage @13:38:02.56 Elbow.reconfigure :loose @13:38:02.65 Forearm.reconfigure :loose @13:38:02.69 Forearm.seek :garage @13:38:03.66 Hand.close @13:38:04.43 Forearm.seek :retract @13:38:05.45 Elbow.reconfigure :tight @13:38:05.55 Elbow.seek CC @13:38:07.16 upsync @13:38:08.51
Elbow.reconfigure :loose @13:38:08.61 Forearm.reconfigure :tight @13:38:08.65 Forearm.seek CC @13:38:09.83 CC.seek :closed @13:38:14.44 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.29 @13:38:14.51 Hand.open @13:38:15.12 Forearm.reconfigure :loose @13:38:15.17 Forearm.seek :retract @13:38:16.11 Flushing the Collection Valve::Manifold @13:38:16.23 CSV.dial CSR @13:38:17.55 Pulling 5ml of CSR.flush @13:38:17.56 CSR.select :flush @13:38:17.67 CS.reconfigure :default @13:38:17.71 CS.seek 5ml @13:38:31.52 delay 5 seconds @13:38:36.53 CSR.select :air @13:38:36.68 CS.reconfigure :fast @13:38:36.73 CS.seek 7.5ml @13:38:43.51 CSV.dial :waste @13:38:44.78 CS.reconfigure :default @13:38:44.83 CS.seek 0ml @13:39:04.98 Purging external Intake @13:39:05.09 CSV.dial :flush @13:39:05.53 CS.reconfigure :fast @13:39:05.58 CS.seek 10ml @13:39:29.76 delay 5 seconds @13:39:34.78 CSV.dial CBV @13:39:34.81
CBV.dial CSV @13:39:35.56 CTV.dial :intake @13:39:36.34 Intake.select :open @13:39:36.44 CS.seek 0ml @13:39:59.70 Intake.select :close @13:39:59.80 CTV.dial :air @13:40:00.56 Flushing upper manifold @13:40:00.67 CSV.dial :flush @13:40:01.39 CS.seek 5ml @13:40:13.92 delay 5 seconds @13:40:18.94 CSV.dial :air @13:40:19.43 CS.seek 7.001ml @13:40:24.98 CSV.dial CBV @13:40:25.00
CBV.dial CSV @13:40:26.01 CTV.dial :air @13:40:26.03 CS.seek 0ml @13:40:42.51 delay 7 seconds @13:40:49.54 CS.seek 3ml @13:40:57.54 CSV.dial :waste @13:40:57.99 CS.seek 0ml @13:41:05.77 CSV.dial :flush @13:41:06.21 CS.seek 3ml @13:41:14.10 delay 5 seconds @13:41:19.12 CSV.dial :air @13:41:19.60 CS.seek 5ml @13:41:25.07 CSV.dial :air @13:41:25.09 CS.seek 0ml @13:41:37.32 delay 7 seconds @13:41:44.35 CS.seek 3ml @13:41:52.35 CSV.dial :waste @13:41:53.08 CS.seek 0ml @13:42:00.76 Garaging FlushPuck in Collection Clamp @13:42:00.83 Elbow.reconfigure :tight @13:42:00.87 Elbow.seek CC @13:42:01.13 Elbow.reconfigure :loose @13:42:01.22 Forearm.seek CC @13:42:02.15 Hand.close @13:42:02.81 CC.seek :open @13:42:07.46 Forearm.seek :retract @13:42:08.47 Elbow.reconfigure :tight @13:42:08.51 Elbow.seek :garage @13:42:11.43 Elbow.reconfigure :loose @13:42:11.51 Forearm.reconfigure :tight @13:42:11.55 Forearm.seek :garage @13:42:12.54 Hand.open @13:42:13.62 Forearm.reconfigure :loose @13:42:13.66 Forearm.seek :retract @13:42:14.64 da sampling at most 1000ml, wcr at most 1000ml @13:42:14.68 Loading sh1 puck from tube 2 into Collection Clamp @13:42:14.69 DAfans.email "Started da in tube 2 at 13:42:14UTC06-Dec-22\n{1 => 20,\n 2 => 13,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@13:37:59, 15% flow, 22.3C, 26% humidity, 15.0psia, 13.783V, 0.272A, 0.260A avg, 3.75W",Subject: "da sampling at most 1000ml, wcr at most 1000ml" @13:42:14.94
SC.reconfigure :tight @13:42:14.98 SC.seek 2 @13:42:20.22 upsync @13:42:21.67
SC.configure :limp @13:42:21.88 Elbow.reconfigure :tight @13:42:21.92 Elbow.seek 2 @13:42:23.92 Forearm.reconfigure :tight @13:42:23.96 Forearm.seek 2 + 450 counts @13:42:24.96 SE.reconfigure :pick @13:42:25.01 SE.seek :beamBreak @13:42:27.28 SE.configure :default @13:42:27.43 Elbow.seek 2 @13:42:27.59 Forearm.seek 2 + 300 counts @13:42:27.92 Forearm.reconfigure :loose @13:42:27.96 Hand.close @13:42:28.58 SE.seek :down @13:42:28.77
Forearm.seek :retract @13:42:29.72 Elbow.seek CC @13:42:30.54 SE.stop @13:42:31.32
Elbow.reconfigure :loose @13:42:31.41 Forearm.reconfigure :tight @13:42:31.46 Forearm.seek CC @13:42:32.71 CC.seek :closed @13:42:37.27 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.27 @13:42:37.28 Hand.open @13:42:37.99 Forearm.reconfigure :loose @13:42:38.04 Forearm.seek :retract @13:42:39.03 collectSample 1000ml w/bubble point of 24psi @13:42:39.04 Priming 7ml @13:42:39.05 Intake.select :close @13:42:39.06 Exhaust.select :close @13:42:39.08 SS.hold @13:42:39.28 Calibrating pressure and temperature sensors @13:42:39.31 CTV.dial :air @13:42:39.32 SSV.dial :spit,avoiding: :exhaust @13:42:39.34 SS.reconfigure :fast @13:42:39.38 SS.seek 5ml @13:42:42.23 SSV.dial CBV @13:42:42.27
CBV.dial SSV @13:42:43.56 delay 3.8 seconds @13:42:47.48 SSV.dial :spit @13:42:48.80 SS.seek 0ml @13:42:51.58 SSV.dial CBV @13:42:52.87 CTV.dial :intake @13:42:53.64 SSV.dial CBV @13:42:53.66
CBV.dial SSV @13:42:53.67 Allowing 40s for 7.0ml of remaining 7.0ml @13:42:53.68 Intake.select :open @13:42:55.83 Intake @15.4psia, vacuum from 9.9 to 12.9psi @13:42:55.85 SS.reconfigure :inhaling 2.20ml/s @13:42:55.89 SS.seek 7ml @13:43:02.99 Stroke completed -- waiting for pressure to equalize ... @13:43:03.00 SS.reconfigure :exhale @13:43:03.04 SS.hold @13:43:05.53 Intake.select :close @13:43:05.67 SSV.dial :exhaust @13:43:06.43 Exhaust.select :open @13:43:06.53 SS.seek 0ml @13:43:10.44 Exhaust.select :close @13:43:10.55 Sampling 1000.0ml Intake.select :close @13:43:10.56 Exhaust.select :close @13:43:10.57 SS.reconfigure :default @13:43:10.61 SS.hold @13:43:10.79 CTV.dial :intake @13:43:10.81 SSV.dial CBV @13:43:10.83
CBV.dial SSV @13:43:11.60 Allowing 130s for 25.0ml of remaining 1000.0ml @13:43:11.61 Intake.select :open @13:43:13.76 Intake @15.7psia, vacuum from 10.0 to 13.0psi @13:43:13.78 SS.reconfigure :inhaling 2.20ml/s @13:43:13.82 SS.seek 25ml @13:43:24.14 Vacuum too high -- waiting up to 40 seconds @13:43:24.15 SS.reconfigure :exhale @13:43:24.19 SS.hold @13:43:28.50 Intake @14.9psia, vacuum from 9.4 to 12.4psi @13:43:28.52 SS.reconfigure :inhaling 1.98ml/s @13:43:28.56 SS.seek 25ml @13:43:34.63 Stroke completed -- waiting for pressure to equalize ... @13:43:34.64 SS.reconfigure :exhale @13:43:34.68 SS.hold @13:43:40.26 Intake.select :close @13:43:40.39 SSV.dial :exhaust @13:43:41.13 Exhaust.select :open @13:43:41.24 SS.seek 0ml @13:43:50.02 Exhaust.select :close @13:43:50.12 SSV.dial CBV @13:43:50.15
CBV.dial SSV @13:43:50.90 Allowing 130s for 25.0ml of remaining 975.0ml @13:43:50.91 Intake.select :open @13:43:53.06 Intake @16.6psia, vacuum from 10.0 to 13.0psi @13:43:53.08 SS.reconfigure :inhaling 1.98ml/s @13:43:53.12 SS.seek 25ml @13:44:00.39 Vacuum too high -- waiting up to 40 seconds @13:44:00.40 SS.reconfigure :exhale @13:44:00.44 SS.hold @13:44:01.99 Intake @14.9psia, vacuum from 9.4 to 12.4psi @13:44:02.00 SS.reconfigure :inhaling 1.79ml/s @13:44:02.05 SS.seek 25ml @13:44:12.74 Stroke completed -- waiting for pressure to equalize ... @13:44:12.75 SS.reconfigure :exhale @13:44:12.79 SS.hold @13:44:21.24 Intake.select :close @13:44:21.38 SSV.dial :exhaust @13:44:22.13 Exhaust.select :open @13:44:22.24 SS.seek 0ml @13:44:31.00 Exhaust.select :close @13:44:31.11 SSV.dial CBV @13:44:31.14
CBV.dial SSV @13:44:31.86 Allowing 130s for 25.0ml of remaining 950.0ml @13:44:31.88 Intake.select :open @13:44:34.03 Intake @16.7psia, vacuum from 10.0 to 13.0psi @13:44:34.05 SS.reconfigure :inhaling 1.79ml/s @13:44:34.09 SS.seek 25ml @13:44:41.85 Vacuum too high -- waiting up to 40 seconds @13:44:41.86 SS.reconfigure :exhale @13:44:41.91 SS.hold @13:44:44.15 Intake @15.4psia, vacuum from 9.9 to 12.9psi @13:44:44.16 SS.reconfigure :inhaling 1.61ml/s @13:44:44.20 SS.seek 25ml @13:44:55.58 Stroke completed -- waiting for pressure to equalize ... @13:44:55.59 SS.reconfigure :exhale @13:44:55.63 SS.hold @13:45:03.08 Intake.select :close @13:45:03.21 SSV.dial :exhaust @13:45:03.94 Exhaust.select :open @13:45:04.05 SS.seek 0ml @13:45:13.10 Exhaust.select :close @13:45:13.20 SSV.dial CBV @13:45:13.24
CBV.dial SSV @13:45:13.97 Allowing 130s for 25.0ml of remaining 925.0ml @13:45:13.98 Intake.select :open @13:45:16.13 Intake @16.6psia, vacuum from 10.0 to 13.0psi @13:45:16.15 SS.reconfigure :inhaling 1.61ml/s @13:45:16.19 SS.seek 25ml @13:45:24.36 Vacuum too high -- waiting up to 40 seconds SS.reconfigure :exhale @13:45:24.40 SS.hold @13:45:26.62 Intake @15.2psia, vacuum from 9.7 to 12.7psi @13:45:26.64 SS.reconfigure :inhaling 1.45ml/s @13:45:26.68 SS.seek 25ml @13:45:38.31 Stroke completed -- waiting for pressure to equalize ... @13:45:38.32 SS.reconfigure :exhale @13:45:38.36 SS.hold @13:45:45.89 Intake.select :close @13:45:46.02 SSV.dial :exhaust @13:45:46.77 Exhaust.select :open @13:45:46.87 SS.seek 0ml @13:45:55.67 Exhaust.select :close @13:45:55.78 SSV.dial CBV @13:45:55.83
CBV.dial SSV @13:45:56.53 Allowing 130s for 25.0ml of remaining 900.0ml @13:45:56.54 Intake.select :open @13:45:58.70 Intake @16.7psia, vacuum from 10.0 to 13.0psi @13:45:58.72 SS.reconfigure :inhaling 1.45ml/s @13:45:58.76 SS.seek 25ml @13:46:08.57 Vacuum too high -- waiting up to 40 seconds @13:46:08.58 SS.reconfigure :exhale @13:46:08.62 SS.hold @13:46:10.76 Intake @15.4psia, vacuum from 9.9 to 12.9psi @13:46:10.78 SS.reconfigure :inhaling 1.30ml/s @13:46:10.82 SS.seek 25ml @13:46:23.47 Stroke completed -- waiting for pressure to equalize ... @13:46:23.48 SS.reconfigure :exhale @13:46:23.52 SS.hold @13:46:30.03 Intake.select :close @13:46:30.17 SSV.dial :exhaust @13:46:30.91 Exhaust.select :open @13:46:31.01 SS.seek 0ml @13:46:40.11 Exhaust.select :close @13:46:40.22 SSV.dial CBV @13:46:40.27
CBV.dial SSV @13:46:40.97 Allowing 130s for 25.0ml of remaining 875.0ml @13:46:40.98 Intake.select :open @13:46:43.13 Intake @16.5psia, vacuum from 10.0 to 13.0psi @13:46:43.15 SS.reconfigure :inhaling 1.30ml/s @13:46:43.19 SS.seek 25ml @13:46:57.63 Vacuum too high -- waiting up to 40 seconds @13:46:57.64 SS.reconfigure :exhale @13:46:57.68 SS.hold @13:47:00.24 Intake @15.6psia, vacuum from 10.0 to 13.0psi @13:47:00.26 SS.reconfigure :inhaling 1.17ml/s @13:47:00.30 SS.seek 25ml @13:47:08.53 Stroke completed -- waiting for pressure to equalize ... @13:47:08.54 SS.reconfigure :exhale @13:47:08.58 SS.hold @13:47:14.25 Intake.select :close @13:47:14.39 SSV.dial :exhaust @13:47:15.13 Exhaust.select :open @13:47:15.23 SS.seek 0ml @13:47:24.20 Exhaust.select :close @13:47:24.30 SSV.dial CBV @13:47:24.33
CBV.dial SSV @13:47:25.08 Allowing 130s for 25.0ml of remaining 850.0ml @13:47:25.09 Intake.select :open @13:47:27.24 Intake @16.5psia, vacuum from 10.0 to 13.0psi @13:47:27.26 SS.reconfigure :inhaling 1.17ml/s @13:47:27.30 SS.seek 25ml @13:47:48.23 Vacuum too high -- waiting up to 40 seconds SS.reconfigure :exhale @13:47:48.27 SS.hold @13:47:51.28 Intake @15.7psia, vacuum from 10.0 to 13.0psi @13:47:51.29 SS.reconfigure :inhaling 1.06ml/s @13:47:51.34 SS.seek 25ml @13:47:54.65 Stroke completed -- waiting for pressure to equalize ... @13:47:54.66 SS.reconfigure :exhale @13:47:54.70 SS.hold @13:47:58.78 Intake.select :close @13:47:58.91 SSV.dial :exhaust @13:47:59.66 Exhaust.select :open @13:47:59.77 SS.seek 0ml @13:48:08.42 Exhaust.select :close @13:48:08.52 SSV.dial CBV @13:48:08.54
CBV.dial SSV @13:48:09.30 Allowing 130s for 25.0ml of remaining 825.0ml @13:48:09.31 Intake.select :open @13:48:11.46 Intake @16.5psia, vacuum from 10.0 to 13.0psi @13:48:11.48 SS.reconfigure :inhaling 1.06ml/s @13:48:11.52 SS.seek 25ml @13:48:38.07 Stroke completed -- waiting for pressure to equalize ... @13:48:38.08 SS.reconfigure :exhale @13:48:38.13 SS.hold @13:48:43.99 Intake.select :close @13:48:44.12 SSV.dial :exhaust @13:48:44.87 Exhaust.select :open @13:48:44.98 SS.seek 0ml @13:48:53.75 Exhaust.select :close @13:48:53.85 SSV.dial CBV @13:48:53.87
CBV.dial SSV @13:48:54.62 Allowing 130s for 25.0ml of remaining 800.0ml @13:48:54.63 Intake.select :open @13:48:56.79 Intake @16.5psia, vacuum from 10.0 to 13.0psi @13:48:56.81 SS.reconfigure :inhaling 1.06ml/s @13:48:56.85 SS.seek 25ml @13:49:23.37 Stroke completed -- waiting for pressure to equalize ... @13:49:23.38 SS.reconfigure :exhale @13:49:23.42 SS.hold @13:49:29.91 Intake.select :close @13:49:30.04 SSV.dial :exhaust @13:49:30.79 Exhaust.select :open @13:49:30.90 SS.seek 0ml @13:49:39.87 Exhaust.select :close @13:49:39.97 SSV.dial CBV @13:49:40.00
CBV.dial SSV @13:49:40.75 Allowing 130s for 25.0ml of remaining 775.0ml @13:49:40.76 Intake.select :open @13:49:42.91 Intake @16.4psia, vacuum from 10.0 to 13.0psi @13:49:42.93 SS.reconfigure :inhaling 1.06ml/s @13:49:42.97 SS.seek 25ml @13:50:08.96 Stroke completed -- waiting for pressure to equalize ... @13:50:08.97 SS.reconfigure :exhale @13:50:09.01 SS.hold @13:50:15.41 Intake.select :close @13:50:15.55 SSV.dial :exhaust @13:50:16.29 Exhaust.select :open @13:50:16.40 SS.seek 0ml @13:50:25.39 Exhaust.select :close @13:50:25.49 SSV.dial CBV @13:50:25.51
CBV.dial SSV @13:50:26.27 Allowing 130s for 25.0ml of remaining 750.0ml @13:50:26.28 Intake.select :open @13:50:28.44 Intake @16.5psia, vacuum from 10.0 to 13.0psi @13:50:28.46 SS.reconfigure :inhaling 1.06ml/s @13:50:28.50 SS.seek 25ml @13:50:54.52 Stroke completed -- waiting for pressure to equalize ... @13:50:54.53 SS.reconfigure :exhale @13:50:54.58 SS.hold @13:51:00.99 Intake.select :close @13:51:01.13 SSV.dial :exhaust @13:51:01.87 Exhaust.select :open @13:51:01.98 SS.seek 0ml @13:51:10.74 Exhaust.select :close @13:51:10.85 SSV.dial CBV @13:51:10.88
CBV.dial SSV @13:51:11.62 Allowing 130s for 25.0ml of remaining 725.0ml @13:51:11.63 Intake.select :open @13:51:13.79 Intake @16.4psia, vacuum from 10.0 to 13.0psi @13:51:13.80 SS.reconfigure :inhaling 1.06ml/s @13:51:13.85 SS.seek 25ml @13:51:40.57 Stroke completed -- waiting for pressure to equalize ... @13:51:40.58 SS.reconfigure :exhale @13:51:40.62 SS.hold @13:51:48.07 Intake.select :close @13:51:48.21 SSV.dial :exhaust @13:51:48.93 Exhaust.select :open @13:51:49.04 SS.seek 0ml @13:51:57.79 Exhaust.select :close @13:51:57.89 SSV.dial CBV @13:51:57.91
CBV.dial SSV @13:51:58.65 Allowing 130s for 25.0ml of remaining 700.0ml @13:51:58.66 Intake.select :open @13:52:00.81 Intake @16.4psia, vacuum from 10.0 to 13.0psi @13:52:00.83 SS.reconfigure :inhaling 1.06ml/s @13:52:00.87 SS.seek 25ml @13:52:27.16 Vacuum too high -- waiting up to 40 seconds @13:52:27.17 SS.reconfigure :exhale @13:52:27.21 SS.hold @13:52:30.47 Intake @15.2psia, vacuum from 9.7 to 12.7psi @13:52:30.49 SS.reconfigure :inhaling 0.95ml/s @13:52:30.53 SS.seek 25ml @13:52:31.40 Stroke completed -- waiting for pressure to equalize ... @13:52:31.41 SS.reconfigure :exhale @13:52:31.45 SS.hold @13:52:35.00 Intake.select :close @13:52:35.13 SSV.dial :exhaust @13:52:35.88 Exhaust.select :open @13:52:35.99 SS.seek 0ml @13:52:44.99 Exhaust.select :close @13:52:45.10 SSV.dial CBV @13:52:45.13
CBV.dial SSV @13:52:45.85 Allowing 130s for 25.0ml of remaining 675.0ml @13:52:45.86 Intake.select :open @13:52:48.02 Intake @16.4psia, vacuum from 10.0 to 13.0psi @13:52:48.04 SS.reconfigure :inhaling 0.95ml/s @13:52:48.08 SS.seek 25ml @13:53:16.43 Stroke completed -- waiting for pressure to equalize ... @13:53:16.44 SS.reconfigure :exhale @13:53:16.48 SS.hold @13:53:23.03 Intake.select :close @13:53:23.19 SSV.dial :exhaust @13:53:23.91 Exhaust.select :open @13:53:24.02 SS.seek 0ml @13:53:32.80 Exhaust.select :close @13:53:32.91 SSV.dial CBV @13:53:32.94
CBV.dial SSV @13:53:33.70 Allowing 130s for 25.0ml of remaining 650.0ml @13:53:33.71 Intake.select :open @13:53:35.86 Intake @16.3psia, vacuum from 10.0 to 13.0psi @13:53:35.87 SS.reconfigure :inhaling 0.95ml/s @13:53:35.92 SS.seek 25ml @13:54:04.76 Stroke completed -- waiting for pressure to equalize ... @13:54:04.77 SS.reconfigure :exhale @13:54:04.81 SS.hold @13:54:12.18 Intake.select :close @13:54:12.32 SSV.dial :exhaust @13:54:13.07 Exhaust.select :open @13:54:13.18 SS.seek 0ml @13:54:22.01 Exhaust.select :close @13:54:22.11 SSV.dial CBV @13:54:22.13
CBV.dial SSV @13:54:22.88 Allowing 130s for 25.0ml of remaining 625.0ml @13:54:22.89 Intake.select :open @13:54:25.04 Intake @16.3psia, vacuum from 10.0 to 13.0psi @13:54:25.06 SS.reconfigure :inhaling 0.95ml/s @13:54:25.10 SS.seek 25ml @13:54:54.10 Stroke completed -- waiting for pressure to equalize ... @13:54:54.11 SS.reconfigure :exhale @13:54:54.15 SS.hold @13:55:01.73 Intake.select :close @13:55:01.86 SSV.dial :exhaust @13:55:02.60 Exhaust.select :open @13:55:02.71 SS.seek 0ml @13:55:11.44 Exhaust.select :close @13:55:11.55 SSV.dial CBV @13:55:11.57
CBV.dial SSV @13:55:12.32 Allowing 130s for 25.0ml of remaining 600.0ml @13:55:12.33 Intake.select :open @13:55:14.48 Intake @16.4psia, vacuum from 10.0 to 13.0psi @13:55:14.50 SS.reconfigure :inhaling 0.95ml/s @13:55:14.54 SS.seek 25ml @13:55:43.50 Stroke completed -- waiting for pressure to equalize ... @13:55:43.52 SS.reconfigure :exhale @13:55:43.56 SS.hold @13:55:51.53 Intake.select :close @13:55:51.66 SSV.dial :exhaust @13:55:52.41 Exhaust.select :open @13:55:52.52 SS.seek 0ml @13:56:01.27 Exhaust.select :close @13:56:01.38 SSV.dial CBV @13:56:01.39
CBV.dial SSV @13:56:02.15 Allowing 130s for 25.0ml of remaining 575.0ml @13:56:02.16 Intake.select :open @13:56:04.31 Intake @16.3psia, vacuum from 10.0 to 13.0psi @13:56:04.33 SS.reconfigure :inhaling 0.95ml/s @13:56:04.37 SS.seek 25ml @13:56:33.47 Stroke completed -- waiting for pressure to equalize ... @13:56:33.48 SS.reconfigure :exhale @13:56:33.53 SS.hold @13:56:41.98 Intake.select :close @13:56:42.12 SSV.dial :exhaust @13:56:42.85 Exhaust.select :open @13:56:42.96 SS.seek 0ml @13:56:51.78 Exhaust.select :close @13:56:51.89 SSV.dial CBV @13:56:51.91
CBV.dial SSV @13:56:52.68 Allowing 130s for 25.0ml of remaining 550.0ml @13:56:52.69 Intake.select :open @13:56:54.84 Intake @16.4psia, vacuum from 10.0 to 13.0psi @13:56:54.86 SS.reconfigure :inhaling 0.95ml/s @13:56:54.90 SS.seek 25ml @13:57:16.68 Vacuum too high -- waiting up to 40 seconds @13:57:16.69 SS.reconfigure :exhale @13:57:16.73 SS.hold @13:57:20.29 Intake @15.8psia, vacuum from 10.0 to 13.0psi @13:57:20.30 SS.reconfigure :inhaling 0.87ml/s @13:57:20.35 SS.seek 25ml @13:57:28.64 Stroke completed -- waiting for pressure to equalize ... @13:57:28.65 SS.reconfigure :exhale @13:57:28.69 SS.hold @13:57:34.39 Intake.select :close @13:57:34.53 SSV.dial :exhaust @13:57:35.27 Exhaust.select :open @13:57:35.38 SS.seek 0ml @13:57:44.17 Exhaust.select :close @13:57:44.28 SSV.dial CBV @13:57:44.31
CBV.dial SSV @13:57:45.04 Allowing 130s for 25.0ml of remaining 525.0ml @13:57:45.05 Intake.select :open @13:57:47.20 Intake @16.4psia, vacuum from 10.0 to 13.0psi @13:57:47.22 SS.reconfigure :inhaling 0.87ml/s @13:57:47.26 SS.seek 25ml @13:58:18.81 Stroke completed -- waiting for pressure to equalize ... @13:58:18.82 SS.reconfigure :exhale @13:58:18.86 SS.hold @13:58:27.41 Intake.select :close @13:58:27.55 SSV.dial :exhaust @13:58:28.30 Exhaust.select :open @13:58:28.41 SS.seek 0ml @13:58:37.14 Exhaust.select :close @13:58:37.25 SSV.dial CBV @13:58:37.28
CBV.dial SSV @13:58:38.00 Allowing 130s for 25.0ml of remaining 500.0ml @13:58:38.01 Intake.select :open @13:58:40.16 Intake @16.4psia, vacuum from 10.0 to 13.0psi @13:58:40.18 SS.reconfigure :inhaling 0.87ml/s @13:58:40.22 SS.seek 25ml @13:59:08.90 Vacuum too high -- waiting up to 40 seconds @13:59:08.91 SS.reconfigure :exhale @13:59:08.95 SS.hold @13:59:13.65 Intake @16.1psia, vacuum from 10.0 to 13.0psi @13:59:13.66 SS.reconfigure :inhaling 0.78ml/s @13:59:13.70 SS.seek 25ml @13:59:17.33 Stroke completed -- waiting for pressure to equalize ... @13:59:17.34 SS.reconfigure :exhale @13:59:17.38 SS.hold @13:59:22.25 Intake.select :close @13:59:22.39 SSV.dial :exhaust @13:59:23.11 Exhaust.select :open @13:59:23.22 SS.seek 0ml @13:59:32.17 Exhaust.select :close @13:59:32.28 SSV.dial CBV @13:59:32.33
CBV.dial SSV @13:59:33.05 Allowing 130s for 25.0ml of remaining 475.0ml @13:59:33.06 Intake.select :open @13:59:35.21 Intake @16.4psia, vacuum from 10.0 to 13.0psi @13:59:35.23 SS.reconfigure :inhaling 0.78ml/s @13:59:35.27 SS.seek 25ml @14:00:09.47 Stroke completed -- waiting for pressure to equalize ... @14:00:09.48 SS.reconfigure :exhale @14:00:09.52 SS.hold @14:00:18.06 Intake.select :close @14:00:18.20 SSV.dial :exhaust @14:00:18.94 Exhaust.select :open @14:00:19.04 SS.seek 0ml @14:00:27.76 Exhaust.select :close @14:00:27.87 SSV.dial CBV @14:00:27.90
CBV.dial SSV @14:00:28.63 Allowing 130s for 25.0ml of remaining 450.0ml @14:00:28.64 Intake.select :open @14:00:30.79 Intake @16.4psia, vacuum from 10.0 to 13.0psi @14:00:30.81 SS.reconfigure :inhaling 0.78ml/s @14:00:30.85 SS.seek 25ml @14:01:05.50 Stroke completed -- waiting for pressure to equalize ... @14:01:05.51 SS.reconfigure :exhale @14:01:05.55 SS.hold @14:01:14.11 Intake.select :close @14:01:14.25 SSV.dial :exhaust @14:01:14.98 Exhaust.select :open @14:01:15.09 SS.seek 0ml @14:01:24.23 Exhaust.select :close @14:01:24.33 SSV.dial CBV @14:01:24.36
CBV.dial SSV @14:01:25.11 Allowing 130s for 25.0ml of remaining 425.0ml @14:01:25.12 Intake.select :open @14:01:27.27 Intake @16.4psia, vacuum from 10.0 to 13.0psi @14:01:27.29 SS.reconfigure :inhaling 0.78ml/s @14:01:27.33 SS.seek 25ml @14:02:01.50 Stroke completed -- waiting for pressure to equalize ... @14:02:01.51 SS.reconfigure :exhale @14:02:01.55 SS.hold @14:02:13.04 Intake.select :close @14:02:13.18 SSV.dial :exhaust @14:02:13.92 Exhaust.select :open @14:02:14.03 SS.seek 0ml @14:02:23.01 Exhaust.select :close @14:02:23.12 SSV.dial CBV @14:02:23.17
CBV.dial SSV @14:02:23.87 Allowing 130s for 25.0ml of remaining 400.0ml @14:02:23.89 Intake.select :open @14:02:26.04 Intake @16.4psia, vacuum from 10.0 to 13.0psi @14:02:26.05 SS.reconfigure :inhaling 0.78ml/s @14:02:26.10 SS.seek 25ml @14:02:58.99 Vacuum too high -- waiting up to 40 seconds @14:02:59.00 SS.reconfigure :exhale @14:02:59.04 SS.hold @14:03:04.68 Intake @15.9psia, vacuum from 10.0 to 13.0psi @14:03:04.69 SS.reconfigure :inhaling 0.70ml/s @14:03:04.74 SS.seek 25ml @14:03:06.39 Stroke completed -- waiting for pressure to equalize ... @14:03:06.40 SS.reconfigure :exhale @14:03:06.44 SS.hold @14:03:11.30 Intake.select :close @14:03:11.43 SSV.dial :exhaust @14:03:12.17 Exhaust.select :open @14:03:12.28 SS.seek 0ml @14:03:21.07 Exhaust.select :close @14:03:21.18 SSV.dial CBV @14:03:21.23
CBV.dial SSV @14:03:21.94 Allowing 130s for 25.0ml of remaining 375.0ml @14:03:21.95 Intake.select :open @14:03:24.10 Intake @16.4psia, vacuum from 10.0 to 13.0psi @14:03:24.11 SS.reconfigure :inhaling 0.70ml/s @14:03:24.16 SS.seek 25ml @14:04:02.28 Stroke completed -- waiting for pressure to equalize ... @14:04:02.29 SS.reconfigure :exhale @14:04:02.33 SS.hold @14:04:12.07 Intake.select :close @14:04:12.21 SSV.dial :exhaust @14:04:12.95 Exhaust.select :open @14:04:13.06 SS.seek 0ml @14:04:21.87 Exhaust.select :close @14:04:21.97 SSV.dial CBV @14:04:21.99
CBV.dial SSV @14:04:22.75 Allowing 130s for 25.0ml of remaining 350.0ml @14:04:22.76 Intake.select :open @14:04:24.91 Intake @16.4psia, vacuum from 10.0 to 13.0psi @14:04:24.93 SS.reconfigure :inhaling 0.70ml/s @14:04:24.97 SS.seek 25ml @14:05:03.16 Stroke completed -- waiting for pressure to equalize ... @14:05:03.17 SS.reconfigure :exhale @14:05:03.22 SS.hold @14:05:14.24 Intake.select :close @14:05:14.37 SSV.dial :exhaust @14:05:15.14 Exhaust.select :open @14:05:15.24 SS.seek 0ml @14:05:24.04 Exhaust.select :close @14:05:24.15 SSV.dial CBV @14:05:24.16
CBV.dial SSV @14:05:24.92 Allowing 130s for 25.0ml of remaining 325.0ml @14:05:24.93 Intake.select :open @14:05:27.08 Intake @16.4psia, vacuum from 10.0 to 13.0psi @14:05:27.10 SS.reconfigure :inhaling 0.70ml/s @14:05:27.14 SS.seek 25ml @14:06:05.16 Vacuum too high -- waiting up to 40 seconds @14:06:05.17 SS.reconfigure :exhale @14:06:05.21 SS.hold @14:06:10.74 Intake @16.2psia, vacuum from 10.0 to 13.0psi @14:06:10.75 SS.reconfigure :inhaling 0.63ml/s @14:06:10.80 SS.seek 25ml @14:06:11.73 Stroke completed -- waiting for pressure to equalize ... @14:06:11.74 SS.reconfigure :exhale @14:06:11.78 SS.hold @14:06:16.63 Intake.select :close @14:06:16.77 SSV.dial :exhaust @14:06:17.49 Exhaust.select :open @14:06:17.60 SS.seek 0ml @14:06:26.44 Exhaust.select :close @14:06:26.55 SSV.dial CBV @14:06:26.57
CBV.dial SSV @14:06:27.31 Allowing 130s for 25.0ml of remaining 300.0ml @14:06:27.32 Intake.select :open @14:06:29.47 Intake @16.4psia, vacuum from 10.0 to 13.0psi @14:06:29.49 SS.reconfigure :inhaling 0.63ml/s @14:06:29.53 SS.seek 25ml @14:07:12.01 Stroke completed -- waiting for pressure to equalize ... @14:07:12.02 SS.reconfigure :exhale @14:07:12.06 SS.hold @14:07:23.19 Intake.select :close @14:07:23.33 SSV.dial :exhaust @14:07:24.09 Exhaust.select :open @14:07:24.19 SS.seek 0ml @14:07:32.96 Exhaust.select :close @14:07:33.07 SSV.dial CBV @14:07:33.08
CBV.dial SSV @14:07:33.84 Allowing 130s for 25.0ml of remaining 275.0ml @14:07:33.85 Intake.select :open @14:07:36.00 Intake @16.3psia, vacuum from 10.0 to 13.0psi @14:07:36.02 SS.reconfigure :inhaling 0.63ml/s @14:07:36.06 SS.seek 25ml @14:08:18.10 Stroke completed -- waiting for pressure to equalize ... @14:08:18.11 SS.reconfigure :exhale @14:08:18.15 SS.hold @14:08:30.69 Intake.select :close @14:08:30.83 SSV.dial :exhaust @14:08:31.58 Exhaust.select :open @14:08:31.69 SS.seek 0ml @14:08:40.49 Exhaust.select :close @14:08:40.59 SSV.dial CBV @14:08:40.61
CBV.dial SSV @14:08:41.37 Allowing 130s for 25.0ml of remaining 250.0ml @14:08:41.38 Intake.select :open @14:08:43.53 Intake @16.4psia, vacuum from 10.0 to 13.0psi @14:08:43.55 SS.reconfigure :inhaling 0.63ml/s @14:08:43.59 SS.seek 25ml @14:09:20.29 Vacuum too high -- waiting up to 40 seconds @14:09:20.30 SS.reconfigure :exhale @14:09:20.34 SS.hold @14:09:26.45 Intake @16.7psia, vacuum from 10.0 to 13.0psi @14:09:26.46 SS.reconfigure :inhaling 0.57ml/s @14:09:26.50 SS.seek 25ml @14:09:32.88 Stroke completed -- waiting for pressure to equalize ... @14:09:32.89 SS.reconfigure :exhale @14:09:32.93 SS.hold @14:09:40.40 Intake.select :close @14:09:40.54 SSV.dial :exhaust @14:09:41.27 Exhaust.select :open @14:09:41.38 SS.seek 0ml @14:09:50.49 Exhaust.select :close @14:09:50.59 SSV.dial CBV @14:09:50.61
CBV.dial SSV @14:09:51.38 Allowing 130s for 25.0ml of remaining 225.0ml @14:09:51.39 Intake.select :open @14:09:53.55 Intake @16.4psia, vacuum from 10.0 to 13.0psi @14:09:53.56 SS.reconfigure :inhaling 0.57ml/s @14:09:53.60 SS.seek 25ml @14:10:39.25 Stroke completed -- waiting for pressure to equalize ... @14:10:39.26 SS.reconfigure :exhale @14:10:39.30 SS.hold @14:10:51.95 Intake.select :close @14:10:52.09 SSV.dial :exhaust @14:10:52.83 Exhaust.select :open @14:10:52.94 SS.seek 0ml @14:11:01.72 Exhaust.select :close @14:11:01.82 SSV.dial CBV @14:11:01.85
CBV.dial SSV @14:11:02.60 Allowing 130s for 25.0ml of remaining 200.0ml @14:11:02.61 Intake.select :open @14:11:04.76 Intake @16.4psia, vacuum from 10.0 to 13.0psi @14:11:04.78 SS.reconfigure :inhaling 0.57ml/s @14:11:04.82 SS.seek 25ml @14:11:51.30 Stroke completed -- waiting for pressure to equalize ... @14:11:51.31 SS.reconfigure :exhale @14:11:51.35 SS.hold @14:12:04.05 Intake.select :close @14:12:04.19 SSV.dial :exhaust @14:12:04.94 Exhaust.select :open @14:12:05.05 SS.seek 0ml @14:12:14.06 Exhaust.select :close @14:12:14.17 SSV.dial CBV @14:12:14.22
CBV.dial SSV @14:12:14.92 Allowing 130s for 25.0ml of remaining 175.0ml @14:12:14.93 Intake.select :open @14:12:17.09 Intake @16.3psia, vacuum from 10.0 to 13.0psi @14:12:17.11 SS.reconfigure :inhaling 0.57ml/s @14:12:17.15 SS.seek 25ml @14:13:03.03 Stroke completed -- waiting for pressure to equalize ... @14:13:03.04 SS.reconfigure :exhale @14:13:03.08 SS.hold @14:13:17.43 Intake.select :close @14:13:17.57 SSV.dial :exhaust @14:13:18.30 Exhaust.select :open @14:13:18.41 SS.seek 0ml @14:13:27.51 Exhaust.select :close @14:13:27.62 SSV.dial CBV @14:13:27.64
CBV.dial SSV @14:13:28.39 Allowing 130s for 25.0ml of remaining 150.0ml @14:13:28.40 Intake.select :open @14:13:30.55 Intake @16.5psia, vacuum from 10.0 to 13.0psi @14:13:30.57 SS.reconfigure :inhaling 0.57ml/s @14:13:30.61 SS.seek 25ml @14:14:02.41 Vacuum too high -- waiting up to 40 seconds @14:14:02.42 SS.reconfigure :exhale @14:14:02.46 SS.hold @14:14:08.86 Intake @16.4psia, vacuum from 10.0 to 13.0psi @14:14:08.87 SS.reconfigure :inhaling 0.51ml/s @14:14:08.92 SS.seek 25ml @14:14:24.64 Stroke completed -- waiting for pressure to equalize ... @14:14:24.65 SS.reconfigure :exhale @14:14:24.69 SS.hold @14:14:37.23 Intake.select :close @14:14:37.37 SSV.dial :exhaust @14:14:38.11 Exhaust.select :open @14:14:38.22 SS.seek 0ml @14:14:47.31 Exhaust.select :close @14:14:47.41 SSV.dial CBV @14:14:47.44
CBV.dial SSV @14:14:48.19 Allowing 130s for 25.0ml of remaining 125.0ml @14:14:48.20 Intake.select :open @14:14:50.35 Intake @16.4psia, vacuum from 10.0 to 13.0psi @14:14:50.37 SS.reconfigure :inhaling 0.51ml/s @14:14:50.41 SS.seek 25ml @14:15:41.21 Stroke completed -- waiting for pressure to equalize ... @14:15:41.22 SS.reconfigure :exhale @14:15:41.27 SS.hold @14:15:57.55 Intake.select :close @14:15:57.68 SSV.dial :exhaust @14:15:58.43 Exhaust.select :open @14:15:58.54 SS.seek 0ml @14:16:07.35 Exhaust.select :close @14:16:07.46 SSV.dial CBV @14:16:07.49
CBV.dial SSV @14:16:08.23 Allowing 130s for 25.0ml of remaining 100.0ml @14:16:08.24 Intake.select :open @14:16:10.40 Intake @16.5psia, vacuum from 10.0 to 13.0psi @14:16:10.41 SS.reconfigure :inhaling 0.51ml/s @14:16:10.45 SS.seek 25ml @14:16:52.06 Vacuum too high -- waiting up to 40 seconds @14:16:52.07 SS.reconfigure :exhale @14:16:52.12 SS.hold @14:16:59.57 Intake @16.8psia, vacuum from 10.0 to 13.0psi @14:16:59.59 SS.reconfigure :inhaling 0.47ml/s @14:16:59.63 SS.seek 25ml @14:17:10.66 Stroke completed -- waiting for pressure to equalize ... @14:17:10.67 SS.reconfigure :exhale @14:17:10.72 SS.hold @14:17:21.84 Intake.select :close @14:17:21.98 SSV.dial :exhaust @14:17:22.73 Exhaust.select :open @14:17:22.84 SS.seek 0ml @14:17:31.60 Exhaust.select :close @14:17:31.71 SSV.dial CBV @14:17:31.76
CBV.dial SSV @14:17:32.47 Allowing 130s for 25.0ml of remaining 75.0ml @14:17:32.48 Intake.select :open @14:17:34.63 Intake @16.4psia, vacuum from 10.0 to 13.0psi @14:17:34.64 SS.reconfigure :inhaling 0.47ml/s @14:17:34.69 SS.seek 25ml @14:18:31.49 Stroke completed -- waiting for pressure to equalize ... @14:18:31.50 SS.reconfigure :exhale @14:18:31.54 SS.hold @14:18:47.88 Intake.select :close @14:18:48.01 SSV.dial :exhaust @14:18:48.77 Exhaust.select :open @14:18:48.88 SS.seek 0ml @14:18:57.69 Exhaust.select :close @14:18:57.80 SSV.dial CBV @14:18:57.82
CBV.dial SSV @14:18:58.57 Allowing 130s for 25.0ml of remaining 50.0ml @14:18:58.58 Intake.select :open @14:19:00.73 Intake @16.4psia, vacuum from 10.0 to 13.0psi @14:19:00.75 SS.reconfigure :inhaling 0.47ml/s @14:19:00.79 SS.seek 25ml @14:19:57.52 Stroke completed -- waiting for pressure to equalize ... @14:19:57.53 SS.reconfigure :exhale @14:19:57.57 SS.hold @14:20:13.87 Intake.select :close @14:20:14.00 SSV.dial :exhaust @14:20:14.73 Exhaust.select :open @14:20:14.83 SS.seek 0ml @14:20:23.62 Exhaust.select :close @14:20:23.72 SSV.dial CBV @14:20:23.74
CBV.dial SSV @14:20:24.48 Allowing 130s for 25.0ml of remaining 25.0ml @14:20:24.49 Intake.select :open @14:20:26.64 Intake @16.6psia, vacuum from 10.0 to 13.0psi @14:20:26.66 SS.reconfigure :inhaling 0.47ml/s @14:20:26.70 SS.seek 24.97ml @14:21:05.62 Vacuum too high -- waiting up to 40 seconds @14:21:05.63 SS.reconfigure :exhale @14:21:05.67 SS.hold @14:21:12.06 Intake @16.4psia, vacuum from 10.0 to 13.0psi @14:21:12.08 SS.reconfigure :inhaling 0.43ml/s @14:21:12.12 SS.seek 24.97ml @14:21:32.86 Stroke completed -- waiting for pressure to equalize ... @14:21:32.87 SS.reconfigure :exhale @14:21:32.91 SS.hold @14:21:45.59 Intake.select :close @14:21:45.73 SSV.dial :exhaust @14:21:46.49 Exhaust.select :open @14:21:46.60 SS.seek 0ml @14:21:55.68 Exhaust.select :close @14:21:55.78 Sampled 1000.0ml @14:21:55.79 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @14:21:55.80 Intake.select :close @14:21:55.86 CSV.dial :waste @14:21:55.91 CS.seek 0ml @14:21:56.00 SSV.dial CBV,avoiding: :exhaust @14:21:56.03
CBV.dial SSV @14:21:56.12 CSV.dial :air @14:21:56.86 CS.seek 10ml @14:21:57.87
CTV.dial :air @14:21:58.64 delay 15 seconds @14:22:13.66 SSV.dial :spit,avoiding: :exhaust @14:22:14.97 CTV.dial CSV,avoiding: :intake @14:22:15.00
SS.seek 0ml @14:22:15.11 SSV.dial CBV,avoiding: :exhaust @14:22:21.18 CBV.dial between SSV and waste2 @14:22:21.55 CTV.dial CSV,avoiding: :air @14:22:21.57
CSV.dial CTV @14:22:22.02 CS.reconfigure :pressurize @14:22:22.06 CS.seek 0ml @14:22:40.69 BubblePoint: Intake @39.5psia, Outlet @15.5psia @14:22:40.70 CBV.dial SSV @14:22:41.02 SS.reconfigure :default @14:22:41.06 SS.seek 10ml @14:22:46.58 delay 15 seconds @14:23:01.70 Evacuated: Intake @17.6psia, Outlet @10.0psia @14:23:01.71 SSV.dial :spit,avoiding: :exhaust @14:23:03.03 SS.reconfigure :exhale @14:23:03.07 SS.seek 0ml @14:23:08.37 SS.reconfigure :fast @14:23:08.41 SS.seek 5ml @14:23:11.26 SSV.dial CBV,avoiding: :exhaust @14:23:11.28
CBV.dial SSV @14:23:12.59 CTV.dial :air,avoiding: :intake @14:23:13.91 CSV.dial CTV @14:23:13.94
SS.seek 10ml @14:23:14.93 CSV.dial :air @14:23:15.38 CS.reconfigure :fast @14:23:15.42 CS.seek 0ml @14:23:16.43
SSV.dial :spit,avoiding: :exhaust @14:23:17.73 SS.reconfigure :exhale @14:23:17.77 SS.seek 0ml @14:23:22.87 SS.reconfigure :fast @14:23:22.91 SS.seek 5ml @14:23:23.06 CS.stop @14:23:25.76 SSV.dial CBV,avoiding: :exhaust @14:23:25.78
CBV.dial SSV @14:23:28.11 SSV.dial :spit,avoiding: :exhaust @14:23:29.40 SS.reconfigure :default @14:23:29.44 SS.seek 0ml @14:23:32.70 SS.stop @14:23:32.74 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @14:23:32.75 Intake.select :close @14:23:32.82 CSV.dial :waste @14:23:32.93 SSV.dial CBV,avoiding: :exhaust @14:23:32.96
CBV.dial SSV @14:23:33.58 CS.seek 0ml @14:23:33.65 CSV.dial :air @14:23:34.26
CTV.dial :air @14:23:34.27 delay 15 seconds @14:23:34.38 CS.seek 10ml @14:23:49.29
SSV.dial :spit,avoiding: :exhaust @14:23:50.59 CTV.dial CSV,avoiding: :intake @14:23:50.64
SS.reconfigure :exhale @14:23:50.70 SS.seek 0ml @14:23:51.50 SSV.dial CBV,avoiding: :exhaust @14:23:58.59 CBV.dial between SSV and waste2 @14:23:58.99 CTV.dial CSV,avoiding: :air @14:23:59.00
CSV.dial CTV @14:23:59.46 CS.reconfigure :pressurize @14:23:59.50 CS.seek 0ml @14:24:20.59 BubblePoint: Intake @38.4psia, Outlet @14.4psia @14:24:20.60 CBV.dial SSV @14:24:20.92 SS.reconfigure :default @14:24:20.97 SS.seek 10ml @14:24:26.58 delay 15 seconds @14:24:41.70 Evacuated: Intake @18.8psia, Outlet @11.7psia @14:24:41.71 SSV.dial :spit,avoiding: :exhaust @14:24:43.01 SS.reconfigure :exhale @14:24:43.05 SS.seek 0ml @14:24:48.37 SS.reconfigure :fast @14:24:48.41 SS.seek 5ml @14:24:51.26 SSV.dial CBV,avoiding: :exhaust @14:24:51.29
CBV.dial SSV @14:24:52.58 CTV.dial :air,avoiding: :intake @14:24:53.91 CSV.dial CTV @14:24:53.94
SS.seek 10ml @14:24:54.93 CSV.dial :air @14:24:55.39 CS.reconfigure :fast @14:24:55.44 CS.seek 0ml @14:24:56.39
SSV.dial :spit,avoiding: :exhaust @14:24:57.71 SS.reconfigure :exhale @14:24:57.75 SS.seek 0ml @14:25:00.68 CS.stop @14:25:02.76
SS.reconfigure :fast @14:25:02.80 SS.seek 5ml @14:25:05.94 SSV.dial CBV,avoiding: :exhaust @14:25:05.96
CBV.dial SSV @14:25:08.28 SSV.dial :spit,avoiding: :exhaust @14:25:09.57 SS.reconfigure :default @14:25:09.61 SS.seek 0ml @14:25:12.72 SS.stop @14:25:12.75 Lysing: Adding 2.0ml AqMeOH @14:25:12.76 CBV.dial between CSV and waste1,avoiding: :DBV @14:25:14.25 CTV.dial CSV @14:25:15.54 CSV.dial CSR @14:25:16.33 Pulling 1ml of CSR.AqMeOH @14:25:16.34 CSR.select :AqMeOH @14:25:16.45 CS.reconfigure :default @14:25:16.49 CS.seek 1ml @14:25:19.97 delay 5 seconds @14:25:24.98 CSR.select :air @14:25:25.14 CS.seek 6ml @14:25:38.78 delay 2 seconds @14:25:40.79 CSV.dial :waste2 @14:25:41.24 CS.reconfigure :fast @14:25:41.28 CS.seek 0ml @14:25:55.62 CSV.dial CSR @14:25:56.13 Pulling 2ml of CSR.AqMeOH @14:25:56.14 CSR.select :AqMeOH @14:25:56.24 CS.reconfigure :default @14:25:56.29 CS.seek 2ml @14:26:02.22 delay 5 seconds @14:26:07.23 CSR.select :air @14:26:07.39 CS.seek 5.001ml @14:26:15.93 delay 2 seconds @14:26:17.95 CSV.dial CTV @14:26:18.39 CS.seek 1ml @14:26:29.75 Before lysing Collection Puck Heater at 21.6C/39.1psia @14:26:29.77 Heating Puck.from 2, :sh1 in Collection Clamp to 70C for 10:00 @14:26:29.79 CH.seek 70C,8:00 @14:28:19.24 CH.hold 10:00,70C @14:28:19.41 Begun lysing Collection Puck Heater at 68C/47.1psia @14:38:19.43 Ended lysing Collection Puck Heater at 70C/45.3psia @14:38:19.45 CS.seek 0ml @14:38:23.02 CTV.dial between spare and air,avoiding: :intake @14:38:24.03 CSV.dial :air @14:38:24.47 CS.seek 5ml @14:38:38.26 CSV.dial CTV @14:38:38.72 delay 2 seconds @14:38:40.73 CSV.dial :air @14:38:41.19 delay 2 seconds @14:38:43.20 CSV.dial CTV @14:38:43.65 delay 2 seconds @14:38:45.67 CSV.dial :air @14:38:46.12 delay 2 seconds @14:38:48.14 CS.seek 0ml @14:39:01.78 Puck @72.7psia after final lysis pressurization @14:39:01.80 Recovering Lysate into Collection syringe CSV.dial CBV @14:39:02.79 CBV.dial CSV @14:39:03.17 CS.seek 5ml @14:39:16.82 delay 5 seconds @14:39:21.84 CTV.dial :air @14:39:22.35 CS.seek 7ml @14:39:28.32 delay 5 seconds @14:39:33.34 CSV.dial :air2 @14:39:33.78 CS.seek 10ml @14:39:42.29 CSV.dial between air and CTV,avoiding: CBV @14:39:43.44 Lysing Complete @14:39:43.51 Storing sh1 puck in Collection Clamp to tube 1 @14:39:43.67 SC.reconfigure :tight @14:39:43.71 SC.seek 1 @14:39:47.30 SC.configure :limp @14:39:47.50 Elbow.reconfigure :tight @14:39:47.54 Elbow.seek 1 @14:39:49.28 Forearm.reconfigure :tight @14:39:49.32 Forearm.seek 1 + 450 counts @14:39:50.37 SE.reconfigure :pick @14:39:50.42 SE.seek :beamBreak @14:39:52.21 SE.configure :default @14:39:52.36 Elbow.reconfigure :limp @14:39:52.40 Forearm.reconfigure :loose @14:39:52.44 Forearm.seek 1 @14:39:52.68 Elbow.hold @14:39:52.74 Elbow.reconfigure :tight @14:39:52.83 SE.seek 19.08 Pucks below Top Plate @14:39:53.25 Forearm.force -20 @14:39:53.28 Forearm.seek 1 + 291 counts @14:39:53.57 Forearm.reconfigure :tight @14:39:53.61 Forearm.seek 1 + 450 counts @14:39:53.82 SE.seek 20.08 Pucks below Top Plate @14:39:54.48 Forearm.reconfigure :loose @14:39:54.53 Forearm.seek :retract @14:39:55.45 Elbow.seek CC @14:39:57.13 Elbow.reconfigure :loose @14:39:57.22 Forearm.seek CC @14:39:58.23 Hand.close @14:39:58.89 CC.seek :open @14:40:03.61 Forearm.seek :retract @14:40:04.59 Elbow.reconfigure :tight @14:40:04.63 Elbow.seek 1 @14:40:06.40 Elbow.reconfigure :limp @14:40:06.44 Forearm.seek 1 @14:40:07.42 Elbow.hold @14:40:07.94 Elbow.reconfigure :tight @14:40:07.99 Hand.open @14:40:08.75 Forearm.force -20 @14:40:08.78 Forearm.seek 1 + 310 counts @14:40:09.08 Forearm.reconfigure :tight @14:40:09.12 Forearm.seek 1 + 450 counts @14:40:09.48 SE.seek :down @14:40:10.61
Forearm.reconfigure :loose @14:40:10.66 Forearm.seek :retract @14:40:10.82 SE.stop @14:40:11.69
Tranferring DA @14:40:11.70 Beginning samplePassC @14:40:11.71 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:40:11.90 Elbow.seek FlushPuck::Garage @14:40:13.67 Elbow.reconfigure :loose @14:40:13.75 Forearm.seek :garage @14:40:14.70 Hand.close @14:40:15.37 Forearm.seek :retract @14:40:16.36 Elbow.reconfigure :tight @14:40:16.40 Elbow.seek CC @14:40:19.39 Elbow.reconfigure :loose @14:40:19.47 Forearm.reconfigure :tight @14:40:19.52 Forearm.seek CC @14:40:20.75 CC.seek :closed @14:40:25.26 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.33 @14:40:25.33 Hand.open @14:40:25.95 Forearm.reconfigure :loose @14:40:25.99 Forearm.seek :retract @14:40:26.93 PSV.dial PSR @14:40:28.29 Pulling 1.5ml of PSR.flush @14:40:28.30 PSR.select :flush @14:40:28.41 PS.seek 1.5ml @14:40:44.77 delay 5 seconds @14:40:49.79 PSV.dial :waste1 @14:40:50.26 PS.seek 0ml @14:41:06.42 PSV.dial PSR @14:41:07.00 PSR.select :air @14:41:07.11 PS.seek 2.5ml @14:41:33.46 PRV.dial CBV,avoiding: :ab2 @14:41:33.50
CBV.dial PRV @14:41:35.14 PSV.dial :mixloop @14:41:36.19 CTV.dial :air,avoiding: :intake @14:41:36.20 PTV.dial :PRVmixing @14:41:37.49 PS.seek 0ml @14:42:03.98 PSV.dial :air @14:42:04.44 PS.seek 2.5ml @14:42:30.86 PSV.dial :mixloop @14:42:31.32 PS.seek 0ml @14:42:57.67 PSV.dial :air @14:42:58.13 PS.seek 2.5ml @14:43:24.54 PSV.dial :mixloop @14:43:25.00 PS.seek 0ml @14:43:51.33 Garaging FlushPuck in Collection Clamp @14:43:51.40 Elbow.reconfigure :tight @14:43:51.44 Elbow.seek CC @14:43:51.78 Elbow.reconfigure :loose @14:43:51.87 Forearm.seek CC @14:43:52.83 Hand.close @14:43:53.39 CC.seek :open @14:43:57.99 Forearm.seek :retract @14:43:58.93 Elbow.reconfigure :tight @14:43:58.97 Elbow.seek :garage @14:44:01.84 Elbow.reconfigure :loose @14:44:01.93 Forearm.reconfigure :tight @14:44:01.97 Forearm.seek :garage @14:44:02.90 Hand.open @14:44:04.07 Forearm.reconfigure :loose @14:44:04.11 Forearm.seek :retract @14:44:05.05 PSV.dial :mixloop @14:44:05.07 PTV.dial :PRVmixing @14:44:05.08 PRV.dial CBV @14:44:05.10
CBV.dial PRV @14:44:05.12 CTV.dial :air,avoiding: :intake @14:44:05.14 Loading lyfil puck from tube 2 into Collection Clamp @14:44:05.38 SC.reconfigure :tight @14:44:05.42 SC.seek 2 @14:44:09.02 SC.configure :limp @14:44:09.21 Elbow.reconfigure :tight @14:44:09.25 Elbow.seek 2 @14:44:11.15 Forearm.reconfigure :tight @14:44:11.20 Forearm.seek 2 + 450 counts @14:44:12.29 SE.reconfigure :pick @14:44:12.34 SE.seek :beamBreak @14:44:14.64 SE.configure :default @14:44:14.79 Elbow.seek 2 @14:44:14.96 Forearm.seek 2 + 300 counts @14:44:15.19 Forearm.reconfigure :loose @14:44:15.24 Hand.close @14:44:15.90 SE.seek :down @14:44:16.08
Forearm.seek :retract @14:44:17.01 Elbow.seek CC @14:44:17.94 SE.stop @14:44:18.66
Elbow.reconfigure :loose @14:44:18.75 Forearm.reconfigure :tight @14:44:18.79 Forearm.seek CC @14:44:20.04 CC.seek :closed @14:44:24.55 Collection Clamp onSpring/offSpring for 13/27 samples, on/off torque 2.05 @14:44:24.56 Hand.open @14:44:25.20 Forearm.reconfigure :loose @14:44:25.24 Forearm.seek :retract @14:44:26.24 CTV.dial CSV,avoiding: :intake @14:44:27.58 CSV.dial CTV @14:44:28.01 PS.seek 2.5ml @14:44:54.45 CS.seek 4.5ml @14:45:09.54 delay 8 seconds @14:45:17.56 PSV.dial :air @14:45:18.03 delay 2 seconds @14:45:20.04 PSV.dial :waste2 @14:45:20.78 PS.seek 0ml @14:45:47.10 CBV.dial between PRV and waste1 @14:45:47.43 CSV.dial :air @14:45:47.87 CS.seek 10ml @14:46:02.82 CSV.dial CTV @14:46:03.26 CS.seek 0ml @14:46:29.85 CBV.dial :waste1 @14:46:30.13 delay 5 seconds @14:46:35.15 CTV.dial :air,avoiding: :intake @14:46:36.53 Storing lyfil puck in Collection Clamp to tube 1 @14:46:36.69 SC.reconfigure :tight @14:46:36.73 SC.seek 1 @14:46:40.58 SC.configure :limp @14:46:40.77 Elbow.reconfigure :tight @14:46:40.81 Elbow.seek 1 @14:46:42.63 Forearm.reconfigure :tight @14:46:42.67 Forearm.seek 1 + 450 counts @14:46:43.71 SE.reconfigure :pick @14:46:43.75 SE.seek :beamBreak @14:46:45.56 SE.configure :default @14:46:46.18 Elbow.reconfigure :limp @14:46:46.22 Forearm.reconfigure :loose @14:46:46.26 Forearm.seek 1 @14:46:46.52 Elbow.hold @14:46:46.58 Elbow.reconfigure :tight @14:46:46.67 SE.seek 20.08 Pucks below Top Plate @14:46:47.17 Forearm.force -20 @14:46:47.21 Forearm.seek 1 + 304 counts @14:46:47.50 Forearm.reconfigure :tight @14:46:47.54 Forearm.seek 1 + 450 counts @14:46:47.75 SE.seek 21.08 Pucks below Top Plate @14:46:48.31 Forearm.reconfigure :loose @14:46:48.35 Forearm.seek :retract @14:46:49.29 Elbow.seek CC @14:46:50.97 Elbow.reconfigure :loose @14:46:51.07 Forearm.seek CC @14:46:52.03 Hand.close @14:46:52.63 CC.seek :open @14:46:57.34 Forearm.seek :retract @14:46:58.34 Elbow.reconfigure :tight @14:46:58.38 Elbow.seek 1 @14:47:00.18 Elbow.reconfigure :limp @14:47:00.22 Forearm.seek 1 @14:47:01.21 Elbow.hold @14:47:01.27 Elbow.reconfigure :tight @14:47:01.31 Hand.open @14:47:01.99 Forearm.force -20 @14:47:02.03 Forearm.seek 1 + 302 counts @14:47:02.33 Forearm.reconfigure :tight @14:47:02.37 Forearm.seek 1 + 450 counts @14:47:02.63 SE.seek :down @14:47:03.76
Forearm.reconfigure :loose @14:47:03.80 Forearm.seek :retract @14:47:03.93 SE.stop @14:47:04.71
PSV.dial :air @14:47:05.45 PS.seek 2.5ml @14:47:31.85 delay 1 second @14:47:32.87 PSV.dial :mixloop @14:47:33.29 delay 1 second @14:47:34.30 PSV.dial :air @14:47:34.77 delay 1 second @14:47:35.79 PSV.dial :mixloop @14:47:36.24 delay 1 second @14:47:37.26 PSV.dial :air @14:47:37.73 delay 1 second @14:47:38.75 PS.seek 0ml @14:48:05.13 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @14:48:05.31 Elbow.seek FlushPuck::Garage @14:48:07.03 Elbow.reconfigure :loose @14:48:07.11 Forearm.seek :garage @14:48:08.10 Hand.close @14:48:08.87 Forearm.seek :retract @14:48:09.85 Elbow.reconfigure :tight @14:48:09.89 Elbow.seek PC @14:48:11.14 Elbow.reconfigure :loose @14:48:11.22 Forearm.reconfigure :tight @14:48:11.27 Forearm.seek PC @14:48:12.51 PC.seek :closed @14:48:17.13 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.58 @14:48:17.20 Hand.open @14:48:17.81 Forearm.reconfigure :loose @14:48:18.33 Forearm.seek :retract @14:48:19.31 PRV.dial :air @14:48:19.76 PSV.dial :mixloop @14:48:20.29 PS.seek 0.3ml @14:48:24.13 PSV.dial PSR @14:48:25.34 Pulling 0.4ml of PSR.air @14:48:25.35 PSR.select :air @14:48:25.38 PS.seek 0.7ml @14:48:30.29 delay 3 seconds @14:48:33.31 PSR.select :air @14:48:33.33 PS.seek 2.5ml @14:48:52.51 delay 3 seconds @14:48:55.52 PSV.dial :mixloop @14:48:56.54 PTV.dial :mixing @14:48:57.29 PBV.dial :waste2 @14:48:58.24 PS.seek 0ml @14:49:24.60 Garaging FlushPuck in Processing Clamp @14:49:24.67 Elbow.reconfigure :tight @14:49:24.71 Elbow.seek PC @14:49:24.83 Elbow.reconfigure :loose @14:49:24.91 Forearm.seek PC @14:49:25.89 Hand.close @14:49:26.51 PC.seek :open @14:49:31.24 Forearm.seek :retract @14:49:32.21 Elbow.reconfigure :tight @14:49:32.25 Elbow.seek :garage @14:49:33.58 Elbow.reconfigure :loose @14:49:33.67 Forearm.reconfigure :tight @14:49:33.71 Forearm.seek :garage @14:49:34.69 Hand.open @14:49:35.31 Forearm.reconfigure :loose @14:49:35.36 Forearm.seek :retract @14:49:36.32 Loading da puck from tube 2 into Processing Clamp @14:49:36.54 SC.reconfigure :tight @14:49:36.59 SC.seek 2 @14:49:40.12 SC.configure :limp @14:49:40.31 Elbow.reconfigure :tight @14:49:40.35 Elbow.seek 2 @14:49:42.19 Forearm.reconfigure :tight @14:49:42.23 Forearm.seek 2 + 450 counts @14:49:43.30 SE.reconfigure :pick @14:49:43.35 SE.seek :beamBreak @14:49:45.78 SE.configure :default @14:49:45.92 Elbow.seek 2 @14:49:46.14 Forearm.seek 2 + 300 counts @14:49:46.33 Forearm.reconfigure :loose @14:49:46.37 Hand.close @14:49:47.02 SE.seek :down @14:49:47.22
Forearm.seek :retract @14:49:48.18 Elbow.seek PC @14:49:49.17 SE.stop @14:49:50.68
Elbow.reconfigure :loose @14:49:50.77 Forearm.reconfigure :tight @14:49:50.82 Forearm.seek PC @14:49:51.99 PC.seek :closed @14:49:56.60 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.55 @14:49:56.61 Hand.open @14:49:57.32 Forearm.reconfigure :loose @14:49:57.36 Forearm.seek :retract @14:49:58.39 Heating Puck.from 2, :da in Processing Clamp to 28C for 2:00:00 @14:49:58.40 PH.seek 28C,5:00 @14:50:24.85 PH.hold 2:00:00,28C @14:50:25.37 delay 1:30 @14:51:55.41 PSR.select :air @14:51:55.43 PSV.dial PSR @14:51:56.51 Pulling 0.5ml of PSR.wash @14:51:56.52 PSR.select :wash @14:51:56.64 PS.seek 0.5ml @14:52:02.60 delay 5 seconds @14:52:07.62 PSR.select :air @14:52:07.73 PS.seek 2ml @14:52:23.88 PTV.dial :puck @14:52:24.61 PSV.dial PTV @14:52:25.36 PBV.dial :waste1 @14:52:26.75 PS.seek 1.5ml @14:52:32.75 delay 3 seconds @14:52:35.77 PBV.dial between PSV and waste1 @14:52:36.14 PS.seek 0ml @14:52:52.28 delay 5:00 @14:57:52.30 recoverPSRreagent08 @14:57:52.31 PTV.dial :puck @14:57:52.32 PBV.dial :waste1 @14:57:52.70 delay 30 seconds @14:58:22.72 PBV.dial between PSV and waste1 @14:58:23.09 PSR.select :air @14:58:23.11 PSV.dial PSR @14:58:23.86 PS.reconfigure :fast @14:58:23.90 PS.seek 2.5ml @14:58:47.24 PSV.dial PTV @14:58:48.00 delay 5 seconds @14:58:53.01 PSV.dial PSR @14:58:53.76 delay 5 seconds @14:58:58.78 PSV.dial PTV @14:58:59.53 delay 5 seconds @14:59:04.55 PSV.dial :air @14:59:05.30 PS.seek 0ml @14:59:28.15 PTV.dial :PRVmixing,via: PRV @14:59:29.45 PSV.dial :mixloop @14:59:29.92 PRV.dial :ab1 @14:59:30.67 PS.reconfigure :slow @14:59:30.71 PS.seek 0.15ml @14:59:34.46 delay 2 seconds @14:59:36.48 CBV.dial PRV @14:59:36.50
PRV.dial CBV @14:59:37.06 PS.seek 0.35ml @14:59:41.65 delay 2 seconds @14:59:43.67 CBV.dial between PRV and PSV @14:59:43.98 PRV.dial :air @14:59:44.44 PS.seek 0.9ml @14:59:56.12 PRV.dial :waste2 @14:59:56.84 PS.seek 0.5ml @15:00:05.59 addPMLReagent08 @15:00:05.60 PSV.dial :air @15:00:06.17 PS.reconfigure :default @15:00:06.21 PS.seek 2ml @15:00:22.40 PSV.dial :PML @15:00:22.84 PTV.dial :mixing @15:00:23.59 PBV.dial :waste1 @15:00:24.01 PS.seek 1.85ml @15:00:26.30 PBV.dial between PSV and waste1 @15:00:26.67 PS.seek 0ml @15:00:46.59 delay 2 seconds @15:00:48.61 Beginning samplePassC @15:00:48.62 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @15:00:48.78 Elbow.reconfigure :tight @15:00:48.82 Elbow.seek FlushPuck::Garage @15:00:50.16 Elbow.reconfigure :loose @15:00:50.25 Forearm.seek :garage @15:00:51.26 Hand.close @15:00:51.99 Forearm.seek :retract @15:00:52.93 Elbow.reconfigure :tight @15:00:52.98 Elbow.seek CC @15:00:55.91 Elbow.reconfigure :loose @15:00:56.00 Forearm.reconfigure :tight @15:00:56.04 Forearm.seek CC @15:00:57.25 CC.seek :closed @15:01:01.76 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.35 @15:01:01.84 Hand.open @15:01:03.01 Forearm.reconfigure :loose @15:01:03.06 Forearm.seek :retract @15:01:04.01 PSV.dial PSR @15:01:05.09 Pulling 1.5ml of PSR.flush @15:01:05.10 PSR.select :flush @15:01:05.22 PS.seek 1.5ml @15:01:21.40 delay 5 seconds @15:01:26.42 PSV.dial :waste1 @15:01:26.87 PS.seek 0ml @15:01:43.05 PSV.dial PSR @15:01:43.52 PSR.select :air @15:01:43.64 PS.seek 2.5ml @15:02:09.97 PRV.dial CBV,avoiding: :ab2 @15:02:09.98
CBV.dial PRV @15:02:11.60 PSV.dial :mixloop @15:02:12.62 CTV.dial :air,avoiding: :intake @15:02:12.63 PTV.dial :PRVmixing @15:02:13.39 PS.seek 0ml @15:02:39.71 PSV.dial :air @15:02:40.28 PS.seek 2.5ml @15:03:06.77 PSV.dial :mixloop @15:03:07.23 PS.seek 0ml @15:03:33.61 PSV.dial :air @15:03:34.09 PS.seek 2.5ml @15:04:00.46 PSV.dial :mixloop @15:04:00.93 PS.seek 0ml @15:04:27.43 Garaging FlushPuck in Collection Clamp @15:04:27.50 Elbow.reconfigure :tight @15:04:27.54 Elbow.seek CC @15:04:27.80 Elbow.reconfigure :loose @15:04:27.88 Forearm.seek CC @15:04:28.86 Hand.close @15:04:29.49 CC.seek :open @15:04:34.10 Forearm.seek :retract @15:04:35.15 Elbow.reconfigure :tight @15:04:35.19 Elbow.seek :garage @15:04:38.11 Elbow.reconfigure :loose @15:04:38.20 Forearm.reconfigure :tight @15:04:38.24 Forearm.seek :garage @15:04:39.18 Hand.open @15:04:39.80 Forearm.reconfigure :loose @15:04:39.84 Forearm.seek :retract @15:04:40.81 delayUntil "15:08:48.59" @15:04:40.83 Whole Cell Archive sampling 1000ml @15:04:40.85 Loading wcr puck from tube 2 into Collection Clamp @15:04:41.14 SC.reconfigure :tight @15:04:41.18 SC.seek 2 @15:04:41.55 SC.configure :limp @15:04:41.74 Elbow.reconfigure :tight @15:04:41.79 Elbow.seek 2 @15:04:43.69 Forearm.reconfigure :tight @15:04:43.73 Forearm.seek 2 + 450 counts @15:04:44.77 SE.reconfigure :pick @15:04:44.83 SE.seek :beamBreak @15:04:47.28 SE.configure :default @15:04:47.44 Elbow.seek 2 @15:04:47.67 Forearm.seek 2 + 300 counts @15:04:47.99 Forearm.reconfigure :loose @15:04:48.03 Hand.close @15:04:48.63 SE.seek :down @15:04:48.82 Forearm.seek :retract @15:04:49.76 Elbow.seek CC @15:04:50.81 SE.stop @15:04:51.39 Elbow.reconfigure :loose @15:04:51.49 Forearm.reconfigure :tight @15:04:51.53 Forearm.seek CC @15:04:53.22 CC.seek :closed @15:04:57.78 Collection Clamp onSpring/offSpring for 14/27 samples, on/off torque 2.13 @15:04:57.79 Hand.open @15:04:58.43 Forearm.reconfigure :loose @15:04:58.47 Forearm.seek :retract @15:04:59.43 collectSample 1000ml w/bubble point of 35psi @15:04:59.45 Priming 7ml Intake.select :close @15:04:59.46 Exhaust.select :close @15:04:59.47 SS.hold @15:04:59.67 Calibrating pressure sensors @15:04:59.68 CTV.dial :air @15:04:59.70 SSV.dial :spit,avoiding: :exhaust @15:04:59.71 SS.reconfigure :fast @15:04:59.76 SS.seek 5ml @15:05:02.69 SSV.dial CBV @15:05:02.71 CBV.dial SSV @15:05:04.05 delay 3.8 seconds @15:05:07.97 SSV.dial :spit @15:05:09.27 SS.seek 0ml @15:05:11.86 SSV.dial CBV @15:05:13.18 CTV.dial :intake @15:05:13.93 SSV.dial CBV @15:05:13.95 CBV.dial SSV @15:05:13.97 Allowing 40s for 7.0ml of remaining 7.0ml @15:05:13.98 Intake.select :open @15:05:16.14 Intake @15.4psia, vacuum from 9.9 to 12.9psi @15:05:16.15 SS.reconfigure :inhaling 2.20ml/s @15:05:16.20 SS.seek 7ml @15:05:23.34 Stroke completed -- waiting for pressure to equalize ... @15:05:23.35 SS.reconfigure :exhale @15:05:23.39 SS.hold @15:05:34.65 Intake.select :close @15:05:34.79 SSV.dial :exhaust @15:05:35.53 Exhaust.select :open @15:05:35.64 SS.seek 0ml @15:05:39.68 Exhaust.select :close @15:05:39.79 Sampling 1000.0ml @15:05:39.80 Intake.select :close @15:05:39.81 Exhaust.select :close @15:05:39.82 SS.reconfigure :default @15:05:39.86 SS.hold @15:05:40.06 CTV.dial :intake @15:05:40.08 SSV.dial CBV @15:05:40.11 CBV.dial SSV @15:05:40.84 Allowing 130s for 25.0ml of remaining 1000.0ml @15:05:40.86 Intake.select :open @15:05:43.01 Intake @16.2psia, vacuum from 10.0 to 13.0psi @15:05:43.03 SS.reconfigure :inhaling 2.20ml/s @15:05:43.07 SS.seek 25ml @15:05:49.88 Vacuum too high -- waiting up to 40 seconds @15:05:49.89 SS.reconfigure :exhale @15:05:49.93 SS.hold @15:05:53.23 Intake @15.6psia, vacuum from 10.0 to 13.0psi @15:05:53.25 SS.reconfigure :inhaling 1.98ml/s @15:05:53.29 SS.seek 25ml @15:06:02.90 Stroke completed -- waiting for pressure to equalize ... @15:06:02.91 SS.reconfigure :exhale @15:06:02.96 SS.hold @15:06:11.62 Intake.select :close @15:06:11.76 SSV.dial :exhaust @15:06:12.51 Exhaust.select :open @15:06:12.62 SS.seek 0ml @15:06:21.40 Exhaust.select :close @15:06:21.51 SSV.dial CBV @15:06:21.53 CBV.dial SSV @15:06:22.30 Allowing 130s for 25.0ml of remaining 975.0ml @15:06:22.31 Intake.select :open @15:06:24.46 Intake @16.8psia, vacuum from 10.0 to 13.0psi @15:06:24.48 SS.reconfigure :inhaling 1.98ml/s @15:06:24.53 SS.seek 25ml @15:06:32.35 Vacuum too high -- waiting up to 40 seconds @15:06:32.36 SS.reconfigure :exhale @15:06:32.40 SS.hold @15:06:34.68 Intake @15.6psia, vacuum from 10.0 to 13.0psi @15:06:34.70 SS.reconfigure :inhaling 1.79ml/s @15:06:34.75 SS.seek 25ml @15:06:44.64 Stroke completed -- waiting for pressure to equalize ... @15:06:44.65 SS.reconfigure :exhale @15:06:44.69 SS.hold @15:06:52.10 Intake.select :close @15:06:52.25 SSV.dial :exhaust @15:06:53.00 Exhaust.select :open @15:06:53.11 SS.seek 0ml @15:07:02.20 Exhaust.select :close @15:07:02.31 SSV.dial CBV @15:07:02.33 CBV.dial SSV @15:07:03.09 Allowing 130s for 25.0ml of remaining 950.0ml @15:07:03.10 Intake.select :open @15:07:05.26 Intake @16.9psia, vacuum from 10.0 to 13.0psi @15:07:05.28 SS.reconfigure :inhaling 1.79ml/s @15:07:05.32 SS.seek 25ml @15:07:13.57 Vacuum too high -- waiting up to 40 seconds @15:07:13.58 SS.reconfigure :exhale @15:07:13.62 SS.hold @15:07:16.31 Intake @16.4psia, vacuum from 10.0 to 13.0psi @15:07:16.33 SS.reconfigure :inhaling 1.61ml/s @15:07:16.37 SS.seek 25ml @15:07:26.35 Stroke completed -- waiting for pressure to equalize ... @15:07:26.37 SS.reconfigure :exhale @15:07:26.41 SS.hold @15:07:32.83 Intake.select :close @15:07:32.97 SSV.dial :exhaust @15:07:33.71 Exhaust.select :open @15:07:33.82 SS.seek 0ml @15:07:42.60 Exhaust.select :close @15:07:42.71 SSV.dial CBV @15:07:42.73 CBV.dial SSV @15:07:43.48 Allowing 130s for 25.0ml of remaining 925.0ml @15:07:43.49 Intake.select :open @15:07:45.65 Intake @16.8psia, vacuum from 10.0 to 13.0psi @15:07:45.67 SS.reconfigure :inhaling 1.61ml/s @15:07:45.71 SS.seek 25ml @15:07:55.47 Vacuum too high -- waiting up to 40 seconds @15:07:55.96 SS.reconfigure :exhale @15:07:56.01 SS.hold @15:07:57.13 Intake @14.7psia, vacuum from 9.2 to 12.2psi @15:07:57.15 SS.reconfigure :inhaling 1.45ml/s @15:07:57.20 SS.seek 25ml @15:08:07.77 Stroke completed -- waiting for pressure to equalize ... @15:08:07.79 SS.reconfigure :exhale @15:08:07.83 SS.hold @15:08:14.25 Intake.select :close @15:08:14.39 SSV.dial :exhaust @15:08:15.14 Exhaust.select :open @15:08:15.25 SS.seek 0ml @15:08:24.31 Exhaust.select :close @15:08:24.42 SSV.dial CBV @15:08:24.43 CBV.dial SSV @15:08:25.18 Allowing 130s for 25.0ml of remaining 900.0ml @15:08:25.20 Intake.select :open @15:08:27.35 Intake @16.7psia, vacuum from 10.0 to 13.0psi @15:08:27.37 SS.reconfigure :inhaling 1.45ml/s @15:08:27.42 SS.seek 25ml @15:08:39.00 Vacuum too high -- waiting up to 40 seconds @15:08:39.01 SS.reconfigure :exhale @15:08:39.06 SS.hold @15:08:41.64 Intake @16.0psia, vacuum from 10.0 to 13.0psi @15:08:41.66 SS.reconfigure :inhaling 1.30ml/s @15:08:41.70 SS.seek 25ml @15:08:48.60 recoverPRVreagent08 @15:08:48.61 PTV.dial :mixing @15:08:49.37 PBV.dial :waste1 @15:08:49.73 delay 30 seconds @15:08:51.09 Stroke completed -- waiting for pressure to equalize ... @15:08:51.10 SS.reconfigure :exhale @15:08:51.14 SS.hold @15:08:56.78 Intake.select :close @15:08:56.93 SSV.dial :exhaust @15:08:57.68 Exhaust.select :open @15:08:57.79 SS.seek 0ml @15:09:06.60 Exhaust.select :close @15:09:06.71 SSV.dial CBV @15:09:06.73 CBV.dial SSV @15:09:07.50 Allowing 130s for 25.0ml of remaining 875.0ml @15:09:07.51 Intake.select :open @15:09:09.66 Intake @16.6psia, vacuum from 10.0 to 13.0psi @15:09:09.68 SS.reconfigure :inhaling 1.30ml/s @15:09:09.72 SS.seek 25ml @15:09:19.76 PBV.dial between PSV and waste1 @15:09:20.11 PSV.dial :air @15:09:20.58 PS.reconfigure :fast @15:09:20.63 PS.seek 2.5ml @15:09:25.75 Vacuum too high -- waiting up to 40 seconds @15:09:25.76 SS.reconfigure :exhale @15:09:25.80 SS.hold @15:09:28.88 Intake @16.4psia, vacuum from 10.0 to 13.0psi @15:09:28.90 SS.reconfigure :inhaling 1.17ml/s @15:09:28.95 SS.seek 25ml @15:09:35.98 Stroke completed -- waiting for pressure to equalize ... @15:09:35.99 SS.reconfigure :exhale @15:09:36.03 SS.hold @15:09:40.42 Intake.select :close @15:09:40.57 SSV.dial :exhaust @15:09:41.31 Exhaust.select :open @15:09:41.42 SS.seek 0ml @15:09:44.29 PSV.dial :mixloop @15:09:44.76 delay 2 seconds @15:09:46.78 PSV.dial :air @15:09:47.25 delay 2 seconds @15:09:49.26 PSV.dial :mixloop @15:09:49.73 delay 2 seconds @15:09:50.41 Exhaust.select :close @15:09:50.52 SSV.dial CBV @15:09:50.54 CBV.dial SSV @15:09:51.31 Allowing 130s for 25.0ml of remaining 850.0ml @15:09:51.32 Intake.select :open @15:09:51.75 PSV.dial :air @15:09:52.23 PS.seek 0ml @15:09:53.48 Intake @16.7psia, vacuum from 10.0 to 13.0psi @15:09:53.50 SS.reconfigure :inhaling 1.17ml/s @15:09:53.55 SS.seek 25ml @15:10:14.87 Vacuum too high -- waiting up to 40 seconds @15:10:14.89 SS.reconfigure :exhale @15:10:14.93 SS.hold @15:10:15.23 washCycleDA #1 of 2 @15:10:15.25 PSR.select :air @15:10:15.27 PSV.dial PSR @15:10:16.72 Pulling 0.5ml of PSR.wash @15:10:16.73 PSR.select :wash @15:10:16.85 PS.reconfigure :default @15:10:16.89 PS.seek 0.5ml @15:10:18.62 Intake @16.4psia, vacuum from 10.0 to 13.0psi @15:10:18.64 SS.reconfigure :inhaling 1.06ml/s @15:10:18.68 SS.seek 25ml @15:10:21.55 Stroke completed -- waiting for pressure to equalize ... @15:10:21.57 SS.reconfigure :exhale @15:10:21.61 SS.hold @15:10:22.77 delay 5 seconds @15:10:25.06 Intake.select :close @15:10:25.20 SSV.dial :exhaust @15:10:25.95 Exhaust.select :open @15:10:26.06 SS.seek 0ml @15:10:27.79 PSR.select :air @15:10:27.89 PS.seek 2ml @15:10:35.21 Exhaust.select :close @15:10:35.32 SSV.dial CBV @15:10:35.34 CBV.dial SSV @15:10:36.10 Allowing 130s for 25.0ml of remaining 825.0ml @15:10:36.12 Intake.select :open @15:10:38.27 Intake @16.6psia, vacuum from 10.0 to 13.0psi @15:10:38.29 SS.reconfigure :inhaling 1.06ml/s @15:10:38.34 SS.seek 25ml @15:10:44.04 PTV.dial :puck @15:10:44.78 PSV.dial PTV @15:10:45.53 PBV.dial :waste1 @15:10:45.95 PS.seek 1.5ml @15:10:51.89 delay 3 seconds @15:10:54.90 PBV.dial between PSV and waste1 @15:10:55.27 PS.seek 0ml @15:11:04.51 Stroke completed -- waiting for pressure to equalize ... @15:11:04.52 SS.reconfigure :exhale @15:11:04.56 SS.hold @15:11:10.99 Intake.select :close @15:11:11.13 SSV.dial :exhaust @15:11:11.36 delay 2:00 @15:11:11.88 Exhaust.select :open @15:11:12.48 SS.seek 0ml @15:11:21.51 Exhaust.select :close @15:11:21.61 SSV.dial CBV @15:11:21.63 CBV.dial SSV @15:11:22.40 Allowing 130s for 25.0ml of remaining 800.0ml @15:11:22.41 Intake.select :open @15:11:24.57 Intake @16.5psia, vacuum from 10.0 to 13.0psi @15:11:24.59 SS.reconfigure :inhaling 1.06ml/s @15:11:24.64 SS.seek 25ml @15:11:50.54 Stroke completed -- waiting for pressure to equalize ... @15:11:50.55 SS.reconfigure :exhale @15:11:50.59 SS.hold @15:11:57.01 Intake.select :close @15:11:57.15 SSV.dial :exhaust @15:11:57.90 Exhaust.select :open @15:11:58.01 SS.seek 0ml @15:12:07.14 Exhaust.select :close @15:12:07.25 SSV.dial CBV @15:12:07.27 CBV.dial SSV @15:12:08.02 Allowing 130s for 25.0ml of remaining 775.0ml @15:12:08.04 Intake.select :open @15:12:10.19 Intake @16.6psia, vacuum from 10.0 to 13.0psi @15:12:10.21 SS.reconfigure :inhaling 1.06ml/s @15:12:10.26 SS.seek 25ml @15:12:36.03 Stroke completed -- waiting for pressure to equalize ... @15:12:36.05 SS.reconfigure :exhale @15:12:36.09 SS.hold @15:12:43.48 Intake.select :close @15:12:43.62 SSV.dial :exhaust @15:12:44.36 Exhaust.select :open @15:12:44.47 SS.seek 0ml @15:12:53.72 Exhaust.select :close @15:12:53.83 SSV.dial CBV @15:12:53.85 CBV.dial SSV @15:12:54.62 Allowing 130s for 25.0ml of remaining 750.0ml @15:12:54.63 Intake.select :open @15:12:56.78 Intake @16.6psia, vacuum from 10.0 to 13.0psi @15:12:56.80 SS.reconfigure :inhaling 1.06ml/s @15:12:56.84 SS.seek 25ml @15:13:11.37 recoverPSRreagent08 @15:13:11.40 PTV.dial :puck @15:13:11.42 PBV.dial :waste1 @15:13:11.76 delay 30 seconds @15:13:19.03 Vacuum too high -- waiting up to 40 seconds @15:13:19.04 SS.reconfigure :exhale @15:13:19.08 SS.hold @15:13:22.64 Intake @15.8psia, vacuum from 10.0 to 13.0psi @15:13:22.66 SS.reconfigure :inhaling 0.95ml/s @15:13:22.70 SS.seek 25ml @15:13:26.60 Stroke completed -- waiting for pressure to equalize ... @15:13:26.61 SS.reconfigure :exhale @15:13:26.65 SS.hold @15:13:31.52 Intake.select :close @15:13:31.67 SSV.dial :exhaust @15:13:32.41 Exhaust.select :open @15:13:32.52 SS.seek 0ml @15:13:41.30 Exhaust.select :close @15:13:41.84 PBV.dial between PSV and waste1 @15:13:41.90 SSV.dial CBV @15:13:41.92 CBV.dial SSV @15:13:42.20 PSR.select :air @15:13:42.22 PSV.dial PSR @15:13:42.68 Allowing 130s for 25.0ml of remaining 725.0ml @15:13:42.69 Intake.select :open @15:13:42.98 PS.reconfigure :fast @15:13:43.03 PS.seek 2.5ml @15:13:44.85 Intake @16.6psia, vacuum from 10.0 to 13.0psi @15:13:44.87 SS.reconfigure :inhaling 0.95ml/s @15:13:44.91 SS.seek 25ml @15:14:06.51 PSV.dial PTV @15:14:07.27 delay 5 seconds @15:14:12.29 PSV.dial PSR @15:14:13.04 delay 5 seconds @15:14:13.91 Stroke completed -- waiting for pressure to equalize ... @15:14:13.92 SS.reconfigure :exhale @15:14:13.96 SS.hold @15:14:18.05 PSV.dial PTV @15:14:18.80 delay 5 seconds @15:14:21.36 Intake.select :close @15:14:21.50 SSV.dial :exhaust @15:14:22.25 Exhaust.select :open @15:14:22.36 SS.seek 0ml @15:14:23.82 PSV.dial :air @15:14:24.59 PS.seek 0ml @15:14:31.14 Exhaust.select :close @15:14:31.25 SSV.dial CBV @15:14:31.27 CBV.dial SSV @15:14:32.04 Allowing 130s for 25.0ml of remaining 700.0ml @15:14:32.05 Intake.select :open @15:14:34.20 Intake @16.5psia, vacuum from 10.0 to 13.0psi @15:14:34.22 SS.reconfigure :inhaling 0.95ml/s @15:14:34.26 SS.seek 25ml @15:14:47.88 washCycleDA #2 of 2 @15:14:47.89 PSR.select :air @15:14:47.91 PSV.dial PSR @15:14:49.26 Pulling 0.5ml of PSR.wash @15:14:49.27 PSR.select :wash @15:14:49.38 PS.reconfigure :default @15:14:49.43 PS.seek 0.5ml @15:14:55.38 delay 5 seconds @15:15:00.40 PSR.select :air @15:15:00.52 PS.seek 2ml @15:15:03.42 Stroke completed -- waiting for pressure to equalize ... @15:15:03.43 SS.reconfigure :exhale @15:15:03.48 SS.hold @15:15:11.95 Intake.select :close @15:15:12.10 SSV.dial :exhaust @15:15:12.85 Exhaust.select :open @15:15:12.96 SS.seek 0ml @15:15:16.69 PTV.dial :puck @15:15:16.71 PSV.dial PTV @15:15:17.55 PBV.dial :waste1 @15:15:17.99 PS.seek 1.5ml @15:15:21.85 Exhaust.select :close @15:15:21.96 SSV.dial CBV @15:15:21.98 CBV.dial SSV @15:15:22.74 Allowing 130s for 25.0ml of remaining 675.0ml @15:15:22.75 Intake.select :open @15:15:23.97 delay 3 seconds @15:15:24.91 Intake @16.5psia, vacuum from 10.0 to 13.0psi @15:15:24.92 SS.reconfigure :inhaling 0.95ml/s @15:15:24.97 SS.seek 25ml @15:15:26.99 PBV.dial between PSV and waste1 @15:15:27.37 PS.seek 0ml @15:15:43.48 delay 2:00 @15:15:50.24 Vacuum too high -- waiting up to 40 seconds @15:15:50.26 SS.reconfigure :exhale @15:15:50.30 SS.hold @15:15:54.34 Intake @16.1psia, vacuum from 10.0 to 13.0psi @15:15:54.36 SS.reconfigure :inhaling 0.87ml/s @15:15:54.40 SS.seek 25ml @15:15:58.80 Stroke completed -- waiting for pressure to equalize ... @15:15:58.81 SS.reconfigure :exhale @15:15:58.85 SS.hold @15:16:03.63 Intake.select :close @15:16:03.77 SSV.dial :exhaust @15:16:04.52 Exhaust.select :open @15:16:04.63 SS.seek 0ml @15:16:13.64 Exhaust.select :close @15:16:13.75 SSV.dial CBV @15:16:13.77 CBV.dial SSV @15:16:14.53 Allowing 130s for 25.0ml of remaining 650.0ml @15:16:14.55 Intake.select :open @15:16:16.70 Intake @16.6psia, vacuum from 10.0 to 13.0psi @15:16:16.72 SS.reconfigure :inhaling 0.87ml/s @15:16:16.76 SS.seek 25ml @15:16:47.76 Stroke completed -- waiting for pressure to equalize ... @15:16:47.78 SS.reconfigure :exhale @15:16:47.82 SS.hold @15:16:56.29 Intake.select :close @15:16:56.43 SSV.dial :exhaust @15:16:57.19 Exhaust.select :open @15:16:57.30 SS.seek 0ml @15:17:06.11 Exhaust.select :close @15:17:06.22 SSV.dial CBV @15:17:06.25 CBV.dial SSV @15:17:06.99 Allowing 130s for 25.0ml of remaining 625.0ml @15:17:07.00 Intake.select :open @15:17:09.15 Intake @16.6psia, vacuum from 10.0 to 13.0psi @15:17:09.17 SS.reconfigure :inhaling 0.87ml/s @15:17:09.22 SS.seek 25ml @15:17:40.05 Vacuum too high -- waiting up to 40 seconds @15:17:40.07 SS.reconfigure :exhale @15:17:40.11 SS.hold @15:17:43.49 recoverPSRreagent08 @15:17:43.50 PTV.dial :puck @15:17:43.52 PBV.dial :waste1 @15:17:43.88 delay 30 seconds @15:17:44.32 Intake @16.0psia, vacuum from 10.0 to 13.0psi @15:17:44.34 SS.reconfigure :inhaling 0.78ml/s @15:17:44.39 SS.seek 25ml @15:17:45.47 Stroke completed -- waiting for pressure to equalize ... @15:17:45.48 SS.reconfigure :exhale @15:17:45.52 SS.hold @15:17:49.60 Intake.select :close @15:17:49.74 SSV.dial :exhaust @15:17:50.49 Exhaust.select :open @15:17:50.60 SS.seek 0ml @15:17:59.70 Exhaust.select :close @15:17:59.81 SSV.dial CBV @15:17:59.83 CBV.dial SSV @15:18:01.06 Allowing 130s for 25.0ml of remaining 600.0ml @15:18:01.08 Intake.select :open @15:18:03.23 Intake @16.6psia, vacuum from 10.0 to 13.0psi @15:18:03.25 SS.reconfigure :inhaling 0.78ml/s @15:18:03.29 SS.seek 25ml @15:18:13.90 PBV.dial between PSV and waste1 @15:18:14.27 PSR.select :air @15:18:14.29 PSV.dial PSR @15:18:15.02 PS.reconfigure :fast @15:18:15.07 PS.seek 2.5ml @15:18:37.21 Stroke completed -- waiting for pressure to equalize ... @15:18:37.23 SS.reconfigure :exhale @15:18:37.27 SS.hold @15:18:38.51 PSV.dial PTV @15:18:39.27 delay 5 seconds @15:18:44.28 PSV.dial PSR @15:18:45.03 delay 5 seconds @15:18:45.28 Intake.select :close @15:18:45.42 SSV.dial :exhaust @15:18:46.17 Exhaust.select :open @15:18:46.28 SS.seek 0ml @15:18:50.05 PSV.dial PTV @15:18:50.80 delay 5 seconds @15:18:55.11 Exhaust.select :close @15:18:55.22 SSV.dial CBV @15:18:55.24 CBV.dial SSV @15:18:55.82 PSV.dial :air @15:18:56.00 Allowing 130s for 25.0ml of remaining 575.0ml @15:18:56.01 Intake.select :open @15:18:56.70 PS.seek 0ml @15:18:58.17 Intake @16.6psia, vacuum from 10.0 to 13.0psi @15:18:58.19 SS.reconfigure :inhaling 0.78ml/s @15:18:58.23 SS.seek 25ml @15:19:19.72 getPMLReagent08 @15:19:19.73 PRV.dial :air @15:19:20.17 delay 5 seconds @15:19:25.19 PTV.dial :PRVmixing @15:19:26.50 delay 5 seconds @15:19:31.52 PRV.dial :ab2 @15:19:31.97 PTV.dial :PRVmixing @15:19:31.98 PSV.dial :mixloop @15:19:32.43 PS.reconfigure :default @15:19:32.47 PS.seek 0.3ml @15:19:32.81 Stroke completed -- waiting for pressure to equalize ... @15:19:32.82 SS.reconfigure :exhale @15:19:32.86 SS.hold @15:19:36.37 delay 2 seconds @15:19:38.39 PRV.dial :air @15:19:38.90 PS.seek 0.55ml @15:19:41.32 Intake.select :close @15:19:41.46 SSV.dial :exhaust @15:19:42.21 Exhaust.select :open @15:19:42.31 addPMLReagent08 @15:19:42.32 PSV.dial :air @15:19:42.37 SS.seek 0ml @15:19:42.79 PS.seek 2ml @15:19:51.53 Exhaust.select :close @15:19:51.63 SSV.dial CBV @15:19:51.68 CBV.dial SSV @15:19:52.40 Allowing 130s for 25.0ml of remaining 550.0ml @15:19:52.42 Intake.select :open @15:19:54.57 Intake @16.5psia, vacuum from 10.0 to 13.0psi @15:19:54.59 SS.reconfigure :inhaling 0.78ml/s @15:19:54.63 SS.seek 25ml @15:19:58.59 PSV.dial :PML @15:19:59.06 PTV.dial :mixing @15:19:59.81 PBV.dial :waste1 @15:20:00.25 PS.seek 1.85ml @15:20:02.78 PBV.dial between PSV and waste1 @15:20:03.17 PS.seek 0ml @15:20:22.84 delay 2 seconds @15:20:24.86 delay 8:00 @15:20:28.74 Stroke completed -- waiting for pressure to equalize ... @15:20:28.76 SS.reconfigure :exhale @15:20:29.20 SS.hold @15:20:38.91 Intake.select :close @15:20:39.05 SSV.dial :exhaust @15:20:39.80 Exhaust.select :open @15:20:39.91 SS.seek 0ml @15:20:48.73 Exhaust.select :close @15:20:48.84 SSV.dial CBV @15:20:48.86 CBV.dial SSV @15:20:49.62 Allowing 130s for 25.0ml of remaining 525.0ml @15:20:49.63 Intake.select :open @15:20:51.79 Intake @16.6psia, vacuum from 10.0 to 13.0psi @15:20:51.81 SS.reconfigure :inhaling 0.78ml/s @15:20:51.85 SS.seek 25ml @15:21:20.55 Vacuum too high -- waiting up to 40 seconds @15:21:20.56 SS.reconfigure :exhale @15:21:20.61 SS.hold @15:21:25.49 Intake @16.3psia, vacuum from 10.0 to 13.0psi @15:21:25.51 SS.reconfigure :inhaling 0.70ml/s @15:21:25.56 SS.seek 25ml @15:21:32.43 Stroke completed -- waiting for pressure to equalize ... @15:21:32.44 SS.reconfigure :exhale @15:21:32.48 SS.hold @15:21:39.00 Intake.select :close @15:21:39.14 SSV.dial :exhaust @15:21:39.88 Exhaust.select :open @15:21:40.00 SS.seek 0ml @15:21:48.71 Exhaust.select :close @15:21:48.82 SSV.dial CBV @15:21:48.85 CBV.dial SSV @15:21:49.61 Allowing 130s for 25.0ml of remaining 500.0ml @15:21:49.62 Intake.select :open @15:21:51.77 Intake @16.5psia, vacuum from 10.0 to 13.0psi @15:21:51.79 SS.reconfigure :inhaling 0.70ml/s @15:21:51.83 SS.seek 25ml @15:22:29.83 Stroke completed -- waiting for pressure to equalize ... @15:22:29.85 SS.reconfigure :exhale @15:22:29.89 SS.hold @15:22:41.03 Intake.select :close @15:22:41.17 SSV.dial :exhaust @15:22:41.92 Exhaust.select :open @15:22:42.03 SS.seek 0ml @15:22:50.77 Exhaust.select :close @15:22:50.88 SSV.dial CBV @15:22:50.90 CBV.dial SSV @15:22:51.67 Allowing 130s for 25.0ml of remaining 475.0ml @15:22:51.68 Intake.select :open @15:22:54.34 Intake @16.6psia, vacuum from 10.0 to 13.0psi @15:22:54.36 SS.reconfigure :inhaling 0.70ml/s @15:22:54.40 SS.seek 25ml @15:23:22.11 Vacuum too high -- waiting up to 40 seconds @15:23:22.12 SS.reconfigure :exhale @15:23:22.17 SS.hold @15:23:27.04 SS.reconfigure :inhaling 0.63ml/s @15:23:27.09 SS.seek 25ml @15:23:38.93 Stroke completed -- waiting for pressure to equalize ... @15:23:38.94 SS.reconfigure :exhale @15:23:38.98 SS.hold @15:23:47.46 Intake.select :close @15:23:47.60 SSV.dial :exhaust @15:23:48.35 Exhaust.select :open @15:23:48.46 SS.seek 0ml @15:23:57.31 Exhaust.select :close @15:23:57.42 SSV.dial CBV @15:23:57.44 CBV.dial SSV @15:23:58.20 Allowing 130s for 25.0ml of remaining 450.0ml @15:23:58.21 Intake.select :open @15:24:00.36 Intake @16.5psia, vacuum from 10.0 to 13.0psi @15:24:00.38 SS.reconfigure :inhaling 0.63ml/s @15:24:00.43 SS.seek 25ml @15:24:39.98 Vacuum too high -- waiting up to 40 seconds @15:24:40.00 SS.reconfigure :exhale @15:24:40.04 SS.hold @15:24:46.55 Intake @16.6psia, vacuum from 10.0 to 13.0psi @15:24:46.57 SS.reconfigure :inhaling 0.57ml/s @15:24:46.61 SS.seek 25ml @15:24:49.61 Stroke completed -- waiting for pressure to equalize ... @15:24:49.63 SS.reconfigure :exhale @15:24:49.67 SS.hold @15:24:56.08 Intake.select :close @15:24:56.23 SSV.dial :exhaust @15:24:56.97 Exhaust.select :open @15:24:57.08 SS.seek 0ml @15:25:06.46 Exhaust.select :close @15:25:06.57 SSV.dial CBV @15:25:06.61 CBV.dial SSV @15:25:07.34 Allowing 130s for 25.0ml of remaining 425.0ml @15:25:07.35 Intake.select :open @15:25:09.50 Intake @16.5psia, vacuum from 10.0 to 13.0psi @15:25:09.52 SS.reconfigure :inhaling 0.57ml/s @15:25:09.57 SS.seek 25ml @15:25:55.16 Stroke completed -- waiting for pressure to equalize ... @15:25:55.17 SS.reconfigure :exhale @15:25:55.21 SS.hold @15:26:07.97 Intake.select :close @15:26:08.11 SSV.dial :exhaust @15:26:08.86 Exhaust.select :open @15:26:08.97 SS.seek 0ml @15:26:18.00 Exhaust.select :close @15:26:18.11 SSV.dial CBV @15:26:18.13 CBV.dial SSV @15:26:18.90 Allowing 130s for 25.0ml of remaining 400.0ml @15:26:18.91 Intake.select :open @15:26:21.07 Intake @16.5psia, vacuum from 10.0 to 13.0psi @15:26:21.09 SS.reconfigure :inhaling 0.57ml/s @15:26:21.13 SS.seek 25ml @15:27:00.89 Vacuum too high -- waiting up to 40 seconds @15:27:00.90 SS.reconfigure :exhale @15:27:00.94 SS.hold @15:27:08.43 Intake @16.6psia, vacuum from 10.0 to 13.0psi @15:27:08.45 SS.reconfigure :inhaling 0.51ml/s @15:27:08.49 SS.seek 25ml @15:27:15.52 Stroke completed -- waiting for pressure to equalize ... @15:27:15.53 SS.reconfigure :exhale @15:27:15.57 SS.hold @15:27:24.05 Intake.select :close @15:27:24.19 SSV.dial :exhaust @15:27:24.94 Exhaust.select :open @15:27:25.05 SS.seek 0ml @15:27:33.83 Exhaust.select :close @15:27:33.94 SSV.dial CBV @15:27:33.97 CBV.dial SSV @15:27:34.72 Allowing 130s for 25.0ml of remaining 375.0ml @15:27:34.74 Intake.select :open @15:27:36.89 Intake @16.6psia, vacuum from 10.0 to 13.0psi @15:27:36.91 SS.reconfigure :inhaling 0.51ml/s @15:27:36.95 SS.seek 25ml @15:28:24.88 Vacuum too high -- waiting up to 40 seconds @15:28:24.89 SS.reconfigure :exhale @15:28:24.94 SS.hold @15:28:25.01 recoverPRVreagent08 @15:28:25.02 PTV.dial :mixing @15:28:25.03 PBV.dial :waste1 @15:28:25.41 delay 30 seconds @15:28:31.36 Intake @16.7psia, vacuum from 10.0 to 13.0psi @15:28:31.38 SS.reconfigure :inhaling 0.47ml/s @15:28:31.42 SS.seek 25ml @15:28:35.72 Stroke completed -- waiting for pressure to equalize ... @15:28:35.73 SS.reconfigure :exhale @15:28:35.77 SS.hold @15:28:43.83 Intake.select :close @15:28:43.98 SSV.dial :exhaust @15:28:44.72 Exhaust.select :open @15:28:44.83 SS.seek 0ml @15:28:53.70 Exhaust.select :close @15:28:53.81 SSV.dial CBV @15:28:53.83 CBV.dial SSV @15:28:54.60 Allowing 130s for 25.0ml of remaining 350.0ml @15:28:54.61 Intake.select :open @15:28:55.44 PBV.dial between PSV and waste1 @15:28:55.80 PSV.dial :air @15:28:56.28 PS.reconfigure :fast @15:28:56.32 PS.seek 2.5ml @15:28:56.76 Intake @16.6psia, vacuum from 10.0 to 13.0psi @15:28:56.78 SS.reconfigure :inhaling 0.47ml/s @15:28:56.83 SS.seek 25ml @15:29:19.93 PSV.dial :mixloop @15:29:20.40 delay 2 seconds @15:29:22.41 PSV.dial :air @15:29:22.88 delay 2 seconds @15:29:24.90 PSV.dial :mixloop @15:29:25.37 delay 2 seconds @15:29:27.38 PSV.dial :air @15:29:27.85 PS.seek 0ml @15:29:50.93 washCycleDA #1 of 3 @15:29:50.94 PSR.select :air @15:29:50.95 PSV.dial PSR @15:29:52.43 Pulling 0.5ml of PSR.wash @15:29:52.44 PSR.select :wash @15:29:52.55 PS.reconfigure :default @15:29:52.60 PS.seek 0.5ml @15:29:53.39 Stroke completed -- waiting for pressure to equalize ... @15:29:53.40 SS.reconfigure :exhale @15:29:53.44 SS.hold @15:29:58.58 delay 5 seconds @15:30:03.60 PSR.select :air @15:30:03.71 PS.seek 2ml @15:30:07.72 Intake.select :close @15:30:07.86 SSV.dial :exhaust @15:30:08.61 Exhaust.select :open @15:30:08.72 SS.seek 0ml @15:30:17.47 Exhaust.select :close @15:30:17.58 SSV.dial CBV @15:30:17.61 CBV.dial SSV @15:30:18.34 Allowing 130s for 25.0ml of remaining 325.0ml @15:30:18.36 Intake.select :open @15:30:19.89 PTV.dial :puck @15:30:20.51 Intake @16.5psia, vacuum from 10.0 to 13.0psi @15:30:20.53 SS.reconfigure :inhaling 0.47ml/s @15:30:20.57 SS.seek 25ml @15:30:20.63 PSV.dial PTV @15:30:21.38 PBV.dial :waste1 @15:30:21.81 PS.seek 1.5ml @15:30:27.67 delay 3 seconds @15:30:30.69 PBV.dial between PSV and waste1 @15:30:31.07 PS.seek 0ml @15:30:47.30 delay 2:00 @15:31:16.97 Stroke completed -- waiting for pressure to equalize ... @15:31:16.98 SS.reconfigure :exhale @15:31:17.02 SS.hold @15:31:33.22 Intake.select :close @15:31:33.36 SSV.dial :exhaust @15:31:34.11 Exhaust.select :open @15:31:34.22 SS.seek 0ml @15:31:43.33 Exhaust.select :close @15:31:43.43 SSV.dial CBV @15:31:43.45 CBV.dial SSV @15:31:44.23 Allowing 130s for 25.0ml of remaining 300.0ml @15:31:44.24 Intake.select :open @15:31:46.40 Intake @16.6psia, vacuum from 10.0 to 13.0psi @15:31:46.42 SS.reconfigure :inhaling 0.47ml/s @15:31:46.46 SS.seek 25ml @15:32:36.13 Vacuum too high -- waiting up to 40 seconds @15:32:36.14 SS.reconfigure :exhale @15:32:36.18 SS.hold @15:32:43.67 Intake @16.4psia, vacuum from 10.0 to 13.0psi @15:32:43.69 SS.reconfigure :inhaling 0.43ml/s @15:32:43.73 SS.seek 25ml @15:32:47.32 recoverPSRreagent08 @15:32:47.34 PTV.dial :puck @15:32:47.35 PBV.dial :waste1 @15:32:47.72 delay 30 seconds @15:32:50.64 Stroke completed -- waiting for pressure to equalize ... @15:32:50.65 SS.reconfigure :exhale @15:32:50.69 SS.hold @15:33:01.73 Intake.select :close @15:33:01.87 SSV.dial :exhaust @15:33:02.62 Exhaust.select :open @15:33:02.73 SS.seek 0ml @15:33:11.84 Exhaust.select :close @15:33:11.95 SSV.dial CBV @15:33:11.97 CBV.dial SSV @15:33:12.73 Allowing 130s for 25.0ml of remaining 275.0ml @15:33:12.74 Intake.select :open @15:33:14.90 Intake @16.6psia, vacuum from 10.0 to 13.0psi @15:33:14.92 SS.reconfigure :inhaling 0.43ml/s @15:33:14.96 SS.seek 25ml @15:33:17.74 PBV.dial between PSV and waste1 @15:33:18.10 PSR.select :air @15:33:18.11 PSV.dial PSR @15:33:18.87 PS.reconfigure :fast @15:33:18.91 PS.seek 2.5ml @15:33:42.36 PSV.dial PTV @15:33:43.11 delay 5 seconds @15:33:48.13 PSV.dial PSR @15:33:48.88 delay 5 seconds @15:33:53.89 PSV.dial PTV @15:33:54.64 delay 5 seconds @15:33:59.66 PSV.dial :air @15:34:00.41 PS.seek 0ml @15:34:16.82 Stroke completed -- waiting for pressure to equalize ... @15:34:16.83 SS.reconfigure :exhale @15:34:16.88 SS.hold @15:34:23.55 washCycleDA #2 of 3 @15:34:23.56 PSR.select :air @15:34:23.57 PSV.dial PSR @15:34:24.92 Pulling 0.5ml of PSR.wash @15:34:24.93 PSR.select :wash @15:34:25.05 PS.reconfigure :default @15:34:25.09 PS.seek 0.5ml @15:34:31.52 delay 5 seconds @15:34:33.59 Intake.select :close @15:34:33.73 SSV.dial :exhaust @15:34:34.48 Exhaust.select :open @15:34:34.59 SS.seek 0ml @15:34:36.53 PSR.select :air @15:34:36.65 PS.seek 2ml @15:34:43.41 Exhaust.select :close @15:34:43.53 SSV.dial CBV @15:34:43.56 CBV.dial SSV @15:34:44.29 Allowing 130s for 25.0ml of remaining 250.0ml @15:34:44.31 Intake.select :open @15:34:46.46 Intake @16.6psia, vacuum from 10.0 to 13.0psi @15:34:46.48 SS.reconfigure :inhaling 0.43ml/s @15:34:46.53 SS.seek 25ml @15:34:52.79 PTV.dial :puck @15:34:52.81 PSV.dial PTV @15:34:53.53 PBV.dial :waste1 @15:34:53.95 PS.seek 1.5ml @15:34:59.86 delay 3 seconds @15:35:02.88 PBV.dial between PSV and waste1 @15:35:03.24 PS.seek 0ml @15:35:19.61 delay 2:00 @15:35:44.63 Vacuum too high -- waiting up to 40 seconds @15:35:44.64 SS.reconfigure :exhale @15:35:44.68 SS.hold @15:35:53.23 Intake @17.0psia, vacuum from 10.0 to 13.0psi @15:35:53.25 SS.reconfigure :inhaling 0.38ml/s @15:35:53.30 SS.seek 25ml @15:35:58.66 Stroke completed -- waiting for pressure to equalize ... @15:35:58.67 SS.reconfigure :exhale @15:35:58.72 SS.hold @15:36:09.75 Intake.select :close @15:36:09.89 SSV.dial :exhaust @15:36:10.63 Exhaust.select :open @15:36:10.74 SS.seek 0ml @15:36:19.55 Exhaust.select :close @15:36:19.66 SSV.dial CBV @15:36:19.68 CBV.dial SSV @15:36:20.45 Allowing 130s for 25.0ml of remaining 225.0ml @15:36:20.46 Intake.select :open @15:36:22.61 Intake @16.6psia, vacuum from 10.0 to 13.0psi @15:36:22.63 SS.reconfigure :inhaling 0.38ml/s @15:36:22.67 SS.seek 25ml @15:37:19.63 recoverPSRreagent08 @15:37:19.64 PTV.dial :puck @15:37:19.65 PBV.dial :waste1 @15:37:20.00 delay 30 seconds @15:37:33.72 Stroke completed -- waiting for pressure to equalize ... @15:37:33.74 SS.reconfigure :exhale @15:37:33.78 SS.hold @15:37:50.01 PBV.dial between PSV and waste1 @15:37:50.39 PSR.select :air @15:37:50.43 PSV.dial PSR @15:37:50.57 Intake.select :close @15:37:50.71 SSV.dial :exhaust @15:37:51.21 PS.reconfigure :fast @15:37:51.25 PS.seek 2.5ml @15:37:51.46 Exhaust.select :open @15:37:51.57 SS.seek 0ml @15:38:00.60 Exhaust.select :close @15:38:00.71 SSV.dial CBV @15:38:00.73 CBV.dial SSV @15:38:01.49 Allowing 130s for 25.0ml of remaining 200.0ml @15:38:01.51 Intake.select :open @15:38:03.66 Intake @16.6psia, vacuum from 10.0 to 13.0psi @15:38:03.68 SS.reconfigure :inhaling 0.38ml/s @15:38:03.72 SS.seek 25ml @15:38:14.66 PSV.dial PTV @15:38:15.42 delay 5 seconds @15:38:20.44 PSV.dial PSR @15:38:21.18 delay 5 seconds @15:38:26.20 PSV.dial PTV @15:38:26.95 delay 5 seconds @15:38:31.97 PSV.dial :air @15:38:32.72 PS.seek 0ml @15:38:55.70 washCycleDA #3 of 3 @15:38:55.71 PSR.select :air @15:38:55.72 PSV.dial PSR @15:38:57.08 Pulling 0.5ml of PSR.wash @15:38:57.09 PSR.select :wash @15:38:57.20 PS.reconfigure :default @15:38:57.24 PS.seek 0.5ml @15:39:03.42 delay 5 seconds @15:39:08.44 PSR.select :air @15:39:08.55 PS.seek 2ml @15:39:14.12 Stroke completed -- waiting for pressure to equalize ... @15:39:14.13 SS.reconfigure :exhale @15:39:14.17 SS.hold @15:39:24.73 PTV.dial :puck @15:39:24.74 PSV.dial PTV @15:39:25.47 PBV.dial :waste1 @15:39:25.88 PS.seek 1.5ml @15:39:31.88 delay 3 seconds @15:39:32.41 Intake.select :close @15:39:32.56 SSV.dial :exhaust @15:39:33.30 Exhaust.select :open @15:39:33.41 SS.seek 0ml @15:39:34.90 PBV.dial between PSV and waste1 @15:39:35.27 PS.seek 0ml @15:39:42.25 Exhaust.select :close @15:39:42.36 SSV.dial CBV @15:39:42.38 CBV.dial SSV @15:39:43.15 Allowing 130s for 25.0ml of remaining 175.0ml @15:39:43.16 Intake.select :open @15:39:45.31 Intake @16.7psia, vacuum from 10.0 to 13.0psi @15:39:45.33 SS.reconfigure :inhaling 0.38ml/s @15:39:45.37 SS.seek 25ml @15:39:51.45 delay 2:00 @15:40:50.28 Vacuum too high -- waiting up to 40 seconds @15:40:50.29 SS.reconfigure :exhale @15:40:50.33 SS.hold @15:40:58.77 Intake @16.6psia, vacuum from 10.0 to 13.0psi @15:40:58.79 SS.reconfigure :inhaling 0.34ml/s @15:40:58.84 SS.seek 25ml @15:41:07.53 Stroke completed -- waiting for pressure to equalize ... @15:41:07.97 SS.reconfigure :exhale @15:41:08.09 SS.hold @15:41:22.48 Intake.select :close @15:41:22.62 SSV.dial :exhaust @15:41:23.35 Exhaust.select :open @15:41:23.46 SS.seek 0ml @15:41:32.47 Exhaust.select :close @15:41:32.58 SSV.dial CBV @15:41:32.62 CBV.dial SSV @15:41:33.33 Allowing 130s for 25.0ml of remaining 150.0ml @15:41:33.34 Intake.select :open @15:41:35.50 Intake @16.6psia, vacuum from 10.0 to 13.0psi @15:41:35.52 SS.reconfigure :inhaling 0.34ml/s @15:41:35.56 SS.seek 25ml @15:41:51.47 recoverPSRreagent08 @15:41:51.48 PTV.dial :puck @15:41:51.49 PBV.dial :waste1 @15:41:51.85 delay 30 seconds @15:42:21.88 PBV.dial between PSV and waste1 @15:42:22.23 PSR.select :air @15:42:22.24 PSV.dial PSR @15:42:22.99 PS.reconfigure :fast @15:42:23.04 PS.seek 2.5ml @15:42:46.50 PSV.dial PTV @15:42:47.26 delay 5 seconds @15:42:52.28 PSV.dial PSR @15:42:53.02 delay 5 seconds @15:42:58.04 PSV.dial PTV @15:42:58.52 Stroke completed -- waiting for pressure to equalize ... @15:42:58.53 SS.reconfigure :exhale @15:42:58.57 SS.hold @15:42:58.85 delay 5 seconds @15:43:03.86 PSV.dial :air @15:43:04.62 PS.seek 0ml @15:43:20.91 Intake.select :close @15:43:21.06 SSV.dial :exhaust @15:43:21.80 Exhaust.select :open @15:43:21.91 SS.seek 0ml @15:43:27.78 Gate.power :camera,:ON @15:43:27.80 Mixing DA Substrates @15:43:27.81 PSR.select :air @15:43:27.82 PSV.dial PSR @15:43:29.33 Pulling 0.25ml of PSR.sub1 @15:43:29.35 PSR.select :sub1 @15:43:29.53 PS.reconfigure :default @15:43:29.65 PS.seek 0.25ml @15:43:30.95 Exhaust.select :close @15:43:31.06 SSV.dial CBV @15:43:31.08 CBV.dial SSV @15:43:31.86 Allowing 130s for 25.0ml of remaining 125.0ml @15:43:31.87 Intake.select :open @15:43:33.13 delay 5 seconds @15:43:34.03 Intake @16.9psia, vacuum from 10.0 to 13.0psi @15:43:34.05 SS.reconfigure :inhaling 0.34ml/s @15:43:34.09 SS.seek 25ml @15:43:38.20 Pulling 0.25ml of PSR.sub2 @15:43:38.21 PSR.select :sub2 @15:43:38.32 PS.seek 0.5ml @15:43:41.63 delay 5 seconds @15:43:46.65 PSR.select :air @15:43:46.76 PS.seek 2ml @15:44:02.88 PTV.dial :puck @15:44:02.89 PSV.dial PTV @15:44:03.63 PBV.dial :waste1 @15:44:04.06 PS.seek 1.5ml @15:44:10.06 delay 3 seconds @15:44:13.08 PBV.dial between PSV and waste1 @15:44:13.98 PS.seek 0ml @15:44:30.10 delay 10 seconds @15:44:40.12 recoverPSRreagent08 @15:44:40.13 PTV.dial :puck @15:44:40.14 PBV.dial :waste1 @15:44:40.50 delay 30 seconds @15:44:40.77 Vacuum too high -- waiting up to 40 seconds @15:44:40.79 SS.reconfigure :exhale @15:44:40.83 SS.hold @15:44:50.51 SS.reconfigure :inhaling 0.31ml/s @15:44:50.56 SS.seek 25ml @15:45:08.76 Stroke completed -- waiting for pressure to equalize ... @15:45:08.77 SS.reconfigure :exhale @15:45:08.82 SS.hold @15:45:10.52 PBV.dial between PSV and waste1 @15:45:10.89 PSR.select :air @15:45:10.91 PSV.dial PSR @15:45:11.74 PS.reconfigure :fast @15:45:11.78 PS.seek 2.5ml @15:45:25.09 Intake.select :close @15:45:25.24 SSV.dial :exhaust @15:45:26.00 Exhaust.select :open @15:45:26.11 SS.seek 0ml @15:45:34.93 Exhaust.select :close @15:45:35.04 SSV.dial CBV @15:45:35.06 CBV.dial SSV @15:45:35.50 PSV.dial PTV @15:45:35.85 Allowing 130s for 25.0ml of remaining 100.0ml @15:45:35.86 Intake.select :open @15:45:36.27 delay 5 seconds @15:45:38.01 Intake @16.7psia, vacuum from 10.0 to 13.0psi @15:45:38.03 SS.reconfigure :inhaling 0.31ml/s @15:45:38.08 SS.seek 25ml @15:45:41.28 PSV.dial PSR @15:45:42.05 delay 5 seconds @15:45:47.07 PSV.dial PTV @15:45:47.95 delay 5 seconds @15:45:52.97 PSV.dial :air @15:45:53.76 PS.seek 0ml @15:46:17.31 Additional air clear @15:46:17.32 PSR.select :air @15:46:17.34 PSV.dial PSR @15:46:18.72 Pulling 2ml of PSR.air @15:46:18.73 PSR.select :air @15:46:18.76 PS.reconfigure :default @15:46:18.80 PS.seek 2ml @15:46:40.03 delay 5 seconds @15:46:45.05 PTV.dial :puck @15:46:45.07 PSV.dial PTV @15:46:45.80 PBV.dial :waste1 @15:46:46.23 PS.seek 1.5ml @15:46:52.12 delay 3 seconds @15:46:55.14 PBV.dial between PSV and waste1 @15:46:55.50 delay 1 second @15:46:56.52 PSV.dial :waste1 @15:46:57.58 PS.seek 0ml @15:47:10.77 Stroke completed -- waiting for pressure to equalize ... @15:47:10.79 SS.reconfigure :exhale @15:47:10.83 SS.hold @15:47:13.75 Processing Puck Heater at 28.1C before imaging @15:47:13.76 PH.coast @15:47:13.89 Imaging da puck in Processing Clamp while storing it to tube 1 @15:47:14.65 SC.reconfigure :tight @15:47:14.76 SC.seek 1 @15:47:18.40 SC.configure :limp @15:47:18.68 Elbow.reconfigure :tight @15:47:18.76 Elbow.seek 1 @15:47:20.55 Forearm.reconfigure :tight @15:47:20.60 Forearm.seek 1 + 450 counts @15:47:21.61 SE.reconfigure :pick @15:47:21.66 SE.seek :beamBreak @15:47:23.35 SE.configure :default @15:47:23.50 Elbow.reconfigure :limp @15:47:23.55 Forearm.reconfigure :loose @15:47:23.59 Forearm.seek 1 @15:47:23.85 Elbow.hold @15:47:23.99 Elbow.reconfigure :tight @15:47:24.08 SE.seek 21.08 Pucks below Top Plate @15:47:24.59 Forearm.force -20 @15:47:24.62 Forearm.seek 1 + 317 counts @15:47:24.93 Forearm.reconfigure :tight @15:47:24.97 Forearm.seek 1 + 450 counts @15:47:25.20 SE.seek 22.08 Pucks below Top Plate @15:47:25.75 Forearm.reconfigure :loose @15:47:25.79 Forearm.seek :retract @15:47:26.72 Elbow.seek PC @15:47:29.20 Elbow.reconfigure :loose @15:47:29.28 Forearm.seek PC @15:47:30.30 Hand.close @15:47:30.96 PC.seek :open @15:47:33.72 Intake.select :close @15:47:33.86 SSV.dial :exhaust @15:47:34.62 Exhaust.select :open @15:47:34.73 SS.seek 0ml @15:47:35.72 Forearm.seek :retract @15:47:36.66 Elbow.reconfigure :tight @15:47:36.71 Elbow.seek 1 @15:47:39.30 Forearm.reconfigure :tight @15:47:39.34 Forearm.seek 1 + 450 counts @15:47:40.49 Hand.open @15:47:41.14 SE.seek 21.73 Pucks below Top Plate @15:47:41.40 SE.reconfigure :seal @15:47:41.45 SE.seek 20.76 Pucks below Top Plate @15:47:41.96 Lights, camera, ACTION! @15:47:42.00 PuckCamera: snap -bin=2,2 -auto /var/log/esp/da22dec0615h1000ml.tif @15:47:43.56 Exhaust.select :close @15:47:43.67 SSV.dial CBV @15:47:43.69 CBV.dial SSV @15:47:44.47 Allowing 130s for 25.0ml of remaining 75.0ml @15:47:44.48 Intake.select :open @15:47:46.64 Intake @16.7psia, vacuum from 10.0 to 13.0psi @15:47:46.66 SS.reconfigure :inhaling 0.31ml/s @15:47:46.70 SS.seek 25ml @15:48:01.49 Waiting >20 seconds for da to relinquish FlushPuck @15:48:02.51 SX HX9/16 (USB:06): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 17.23 seconds /var/log/esp/da22dec0615h1000ml.tif: TIFF Upload Complete @15:48:02.53 PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da22dec0615h1000ml15s.tif @15:48:18.19 SX HX9/16 (USB:06): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15 seconds /var/log/esp/midres/da22dec0615h1000ml15s.tif: TIFF Upload Complete @15:48:18.21 PuckCamera: snap -bin=2,2 20.0 /var/log/esp/midres/da22dec0615h1000ml20s.tif @15:48:38.83 SX HX9/16 (USB:06): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 20 seconds /var/log/esp/midres/da22dec0615h1000ml20s.tif: TIFF Upload Complete @15:48:38.84 Gate.power :camera,:OFF @15:48:38.86 DAfans.email "17.23 sec auto-exposure",Subject: "da22dec0615h1000ml images!" @15:48:39.12 Elbow.seek 1 @15:48:39.31 Forearm.seek 1 + 450 counts @15:48:39.45 SE.reconfigure :gently @15:48:39.52 SE.seek 21.48 Pucks below Top Plate @15:48:40.56 Elbow.reconfigure :limp @15:48:40.61 Forearm.reconfigure :loose @15:48:40.67 Forearm.seek 1 @15:48:40.94 Elbow.hold @15:48:41.03 Elbow.reconfigure :tight @15:48:41.08 SE.reconfigure :default @15:48:41.12 SE.seek 21.66 Pucks below Top Plate @15:48:41.53 Forearm.force -20 @15:48:41.57 Forearm.seek 1 + 312 counts @15:48:42.04 Forearm.reconfigure :tight @15:48:42.08 Forearm.seek 1 + 450 counts @15:48:42.34 SE.seek :down @15:48:43.57 Forearm.reconfigure :loose @15:48:43.68 Forearm.seek :retract @15:48:43.81 SE.stop @15:48:44.77 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @15:48:45.09 Elbow.seek FlushPuck::Garage @15:48:47.00 Elbow.reconfigure :loose @15:48:47.21 Forearm.seek :garage @15:48:48.24 Hand.close @15:48:49.12 Forearm.seek :retract @15:48:50.28 Elbow.reconfigure :tight @15:48:50.38 Elbow.seek PC @15:48:51.75 Elbow.reconfigure :loose @15:48:51.95 Forearm.reconfigure :tight @15:48:52.04 Forearm.seek PC @15:48:53.70 PC.seek :closed @15:48:58.53 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.60 @15:48:58.68 Hand.open @15:48:59.49 Forearm.reconfigure :loose @15:48:59.57 Forearm.seek :retract @15:49:00.52 PSR.select :air @15:49:00.56 PSV.dial PSR @15:49:01.20 Pulling 0.5ml of PSR.flush @15:49:01.22 PSR.select :flush @15:49:01.38 PS.seek 0.5ml @15:49:07.44 delay 5 seconds @15:49:12.47 PSR.select :air @15:49:12.61 PS.seek 2.5ml @15:49:14.10 upsync @15:49:19.30 Stroke completed -- waiting for pressure to equalize ... @15:49:19.32 SS.reconfigure :exhale @15:49:19.36 SS.hold @15:49:33.87 delay 1 second @15:49:34.89 PRV.dial :waste2,avoiding: :ab2 @15:49:36.73 PTV.dial :PRVmixing @15:49:38.02 PSV.dial :mixloop @15:49:39.69 PS.seek :empty @15:49:45.78 Intake.select :close @15:49:45.92 SSV.dial :exhaust @15:49:46.67 Exhaust.select :open @15:49:46.78 SS.seek 0ml @15:49:55.82 Exhaust.select :close @15:49:55.93 SSV.dial CBV @15:49:55.95 CBV.dial SSV @15:49:56.71 Allowing 130s for 25.0ml of remaining 50.0ml @15:49:56.73 Intake.select :open @15:49:58.88 Intake @16.9psia, vacuum from 10.0 to 13.0psi @15:49:58.90 SS.reconfigure :inhaling 0.31ml/s @15:49:58.94 SS.seek 25ml @15:50:05.99 PSR.select :air @15:50:06.01 PSV.dial PSR @15:50:07.10 Pulling 0.5ml of PSR.flush @15:50:07.11 PSR.select :flush @15:50:07.22 PS.seek 0.5ml @15:50:13.15 delay 5 seconds @15:50:18.17 PSR.select :air @15:50:18.29 PS.seek 2.5ml @15:50:39.79 delay 1 second @15:50:40.81 PBV.dial :waste1 @15:50:41.15 PTV.dial :mixing @15:50:41.90 PSV.dial :mixloop @15:50:42.95 PS.seek :empty @15:51:09.49 PSV.dial PSR @15:51:10.54 PSR.select :flush @15:51:10.66 PS.seek 1ml @15:51:19.92 Vacuum too high -- waiting up to 40 seconds @15:51:19.93 SS.reconfigure :exhale @15:51:19.97 SS.hold @15:51:21.84 PSR.select :air @15:51:22.01 PS.seek 2.5ml @15:51:31.14 Intake @16.6psia, vacuum from 10.0 to 13.0psi @15:51:31.16 SS.reconfigure :inhaling 0.28ml/s @15:51:31.20 SS.seek 25ml @15:51:38.12 PSV.dial PTV @15:51:38.87 PTV.dial :puck @15:51:39.60 PBV.dial :waste1 @15:51:39.62 PS.seek 0ml @15:51:44.66 Stroke completed -- waiting for pressure to equalize ... @15:51:44.67 SS.reconfigure :exhale @15:51:44.71 SS.hold @15:52:03.07 Intake.select :close @15:52:03.21 SSV.dial :exhaust @15:52:03.96 Exhaust.select :open @15:52:04.07 SS.seek 0ml @15:52:05.91 PSV.dial PSR @15:52:06.65 PSR.select :flush @15:52:06.76 PS.seek 0.5ml @15:52:12.74 PSR.select :air @15:52:12.85 PS.seek 2.5ml @15:52:12.91 Exhaust.select :close @15:52:13.02 SSV.dial CBV @15:52:13.04 CBV.dial SSV @15:52:13.80 Allowing 130s for 25.0ml of remaining 25.0ml @15:52:13.82 Intake.select :open @15:52:16.45 Intake @16.7psia, vacuum from 10.0 to 13.0psi @15:52:16.47 SS.reconfigure :inhaling 0.28ml/s @15:52:16.52 SS.seek 24.96ml @15:52:34.09 PSV.dial PTV @15:52:34.84 PBV.dial PSV @15:52:35.39 PS.seek 0ml @15:53:01.77 PSV.dial PBV @15:53:02.25 PS.seek 2.5ml @15:53:28.58 PSV.dial :waste1 @15:53:29.33 PS.seek 0ml @15:53:55.71 PSV.dial PSR @15:53:56.18 PSR.select :air @15:53:56.20 PS.seek 2.5ml @15:53:59.42 Stroke completed -- waiting for pressure to equalize ... @15:53:59.44 SS.reconfigure :exhale @15:53:59.48 SS.hold @15:54:22.58 PSV.dial PTV @15:54:23.32 PBV.dial PSV @15:54:23.34 PS.seek 0ml @15:54:23.65 SS.reconfigure :default @15:54:23.69 SS.hold @15:54:23.80 Intake.select :close @15:54:23.94 ShallowSampler::Clogged in WCR -- Filtering too Slowly after 1000.0ml @15:54:23.96 SSV.dial :exhaust @15:54:24.71 Exhaust.select :open @15:54:24.81 SS.reconfigure :exhale @15:54:24.86 SS.seek 0ml @15:54:33.92 Exhaust.select :close @15:54:34.03 Sampled 1000.0ml @15:54:34.05 Drying puck before fixing for 20:00 @15:54:34.06 CBV.dial :waste2b @15:54:34.80 delay 1:00 @15:54:49.86 PSV.dial PBV @15:54:50.34 PS.seek 2.5ml @15:55:16.68 PSV.dial :waste1 @15:55:17.42 PS.seek 0ml @15:55:34.82 CSV.dial :air @15:55:35.27 CS.reconfigure :fast @15:55:35.32 CS.seek 10ml @15:55:43.80 PSV.dial PSR @15:55:44.27 PSR.select :flush @15:55:44.39 PS.seek 1ml @15:55:55.52 PSR.select :air @15:55:55.63 PS.seek 2.5ml @15:55:59.56 Pumping wcr puck from tube 2 up to 35psi... @15:55:59.58 CBV.dial SSV @15:56:00.88 CBV.dial between CSV and waste2b @15:56:01.82 CSV.dial between air and CTV @15:56:02.15 CS.seek 7.013ml @15:56:09.72 CTV.dial CSV,avoiding: :air @15:56:09.74 CSV.dial CTV @15:56:10.55 CS.reconfigure :pressurize @15:56:10.60 CS.seek 0ml @15:56:11.77 PSV.dial PTV @15:56:12.61 PTV.dial :puck @15:56:12.63 PBV.dial :waste1 @15:56:13.17 PS.seek 0ml @15:56:28.36 CBV.dial :waste2b @15:56:28.69 CS.reconfigure :default @15:56:28.73 CS.seek 0ml @15:56:30.06 delay 1:00 @15:56:39.49 PSV.dial PSR @15:56:40.22 PSR.select :flush @15:56:40.81 PS.seek 0.5ml @15:56:46.70 PSR.select :air @15:56:46.82 PS.seek 2.5ml @15:57:08.14 PSV.dial PTV @15:57:08.89 PBV.dial PSV @15:57:09.46 PS.seek 0ml @15:57:30.09 CTV.dial :air,avoiding: :intake @15:57:31.41 Fix #1 of 2 (with RNAlater) for 10:00 @15:57:31.42 CSV.dial CSR @15:57:31.91 Pulling 2ml of CSR.RNAlater @15:57:31.92 CSR.select :RNAlater @15:57:32.03 CS.seek 2ml @15:57:35.80 PSV.dial PBV @15:57:36.27 PS.seek 2.5ml @15:57:37.97 delay 5 seconds @15:57:42.98 CSR.select :air @15:57:43.09 CS.reconfigure :fast @15:57:43.14 CS.seek 10ml @15:58:02.61 PSV.dial :waste1 @15:58:02.68 Pumping wcr puck from tube 2 up to 35psi... @15:58:02.70 CBV.dial SSV @15:58:03.35 PS.seek 0ml @15:58:03.99 CBV.dial between CSV and waste2b @15:58:04.93 CSV.dial between air and CTV @15:58:05.52 CS.seek 6.951ml @15:58:13.19 CTV.dial CSV,avoiding: :air @15:58:13.21 CSV.dial CTV @15:58:14.56 CS.reconfigure :pressurize @15:58:14.61 CS.seek 0ml @15:58:29.73 PSV.dial PSR @15:58:30.20 PSR.select :air @15:58:30.23 PS.seek 2.5ml @15:58:31.39 delay 10:00 @15:58:56.76 PSV.dial PTV @15:58:57.50 PBV.dial PSV @15:58:57.52 PS.seek 0ml @15:59:23.92 PSV.dial PBV @15:59:24.40 PS.seek 2.5ml @15:59:50.70 PSV.dial :waste1 @15:59:51.44 PS.seek 0ml @16:00:17.77 Garaging FlushPuck in Processing Clamp @16:00:17.84 Elbow.reconfigure :tight @16:00:17.88 Elbow.seek PC @16:00:18.64 Elbow.reconfigure :loose @16:00:18.73 Forearm.seek PC @16:00:19.68 Hand.close @16:00:20.32 PC.seek :open @16:00:25.03 Forearm.seek :retract @16:00:26.01 Elbow.reconfigure :tight @16:00:26.05 Elbow.seek :garage @16:00:27.41 Elbow.reconfigure :loose @16:00:27.50 Forearm.reconfigure :tight @16:00:27.54 Forearm.seek :garage @16:00:28.54 Hand.open @16:00:29.25 Forearm.reconfigure :loose @16:00:29.30 Forearm.seek :retract @16:08:31.41 CBV.dial :waste2b @16:08:31.74 CS.reconfigure :default @16:08:31.79 CS.seek 0ml @16:08:34.05 delay 1:00 @16:09:34.07 CTV.dial :air,avoiding: :intake @16:09:35.38 Fix #2 of 2 (with RNAlater) for 10:00 @16:09:35.39 CSV.dial CSR @16:09:35.99 Pulling 2ml of CSR.RNAlater @16:09:36.00 CSR.select :RNAlater @16:09:36.11 CS.seek 2ml @16:09:42.05 delay 5 seconds @16:09:47.07 CSR.select :air @16:09:47.18 CS.reconfigure :fast @16:09:47.22 CS.seek 10ml @16:10:06.73 Pumping wcr puck from tube 2 up to 35psi... @16:10:06.75 CBV.dial SSV @16:10:08.03 CBV.dial between CSV and waste2b @16:10:09.44 CSV.dial between air and CTV @16:10:10.02 CS.seek 6.998ml @16:10:17.58 CTV.dial CSV,avoiding: :air @16:10:17.61 CSV.dial CTV @16:10:18.96 CS.reconfigure :pressurize @16:10:19.00 CS.seek 0ml @16:10:34.88 delay 10:00 @16:20:34.90 CBV.dial :waste2b @16:20:35.23 CS.reconfigure :default @16:20:35.27 CS.seek 0ml @16:20:38.85 delay 1:00 @16:21:38.88 CTV.dial :air,avoiding: :intake @16:21:40.22 Storing wcr puck in Collection Clamp to tube 1 @16:21:40.60 Elbow.reconfigure :tight @16:21:40.64 Elbow.seek 1 @16:21:42.50 Forearm.reconfigure :tight @16:21:42.54 Forearm.seek 1 + 450 counts @16:21:43.57 SE.reconfigure :pick @16:21:43.62 SE.seek :beamBreak @16:21:45.33 SE.configure :default @16:21:45.48 Elbow.reconfigure :limp @16:21:45.53 Forearm.reconfigure :loose @16:21:45.57 Forearm.seek 1 @16:21:45.85 Elbow.hold @16:21:45.91 Elbow.reconfigure :tight @16:21:46.01 SE.seek 22.07 Pucks below Top Plate @16:21:46.48 Forearm.force -20 @16:21:46.52 Forearm.seek 1 + 312 counts @16:21:46.82 Forearm.reconfigure :tight @16:21:46.87 Forearm.seek 1 + 450 counts @16:21:47.09 SE.seek 23.07 Pucks below Top Plate @16:21:47.68 Forearm.reconfigure :loose @16:21:47.72 Forearm.seek :retract @16:21:48.68 Elbow.seek CC @16:21:50.36 Elbow.reconfigure :loose @16:21:50.45 Forearm.seek CC @16:21:51.38 Hand.close @16:21:51.93 CC.seek :open @16:21:56.69 Forearm.seek :retract @16:21:57.62 Elbow.reconfigure :tight @16:21:57.66 Elbow.seek 1 @16:21:59.41 Elbow.reconfigure :limp @16:21:59.46 Forearm.seek 1 @16:22:00.44 Elbow.hold @16:22:00.51 Elbow.reconfigure :tight @16:22:00.56 Hand.open @16:22:01.29 Forearm.force -20 @16:22:01.33 Forearm.seek 1 + 312 counts @16:22:01.63 Forearm.reconfigure :tight @16:22:01.67 Forearm.seek 1 + 450 counts @16:22:01.99 SE.seek :down @16:22:03.12 Forearm.reconfigure :loose @16:22:03.17 Forearm.seek :retract @16:22:03.23 SE.stop @16:22:04.10
Starting finalPurge @16:22:04.11 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @16:22:04.76 Elbow.seek FlushPuck::Garage @16:22:06.60 Elbow.reconfigure :loose @16:22:06.70 Forearm.seek :garage @16:22:07.66 Hand.close @16:22:08.41 Forearm.seek :retract @16:22:09.38 Elbow.reconfigure :tight @16:22:09.43 Elbow.seek CC @16:22:12.35 Elbow.reconfigure :loose @16:22:12.44 Forearm.reconfigure :tight @16:22:12.48 Forearm.seek CC @16:22:13.65 CC.seek :closed @16:22:18.16 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.30 @16:22:18.23 Hand.open @16:22:18.86 Forearm.reconfigure :loose @16:22:18.90 Forearm.seek :retract @16:22:19.83 Flushing the Collection Valve::Manifold @16:22:19.95 CSV.dial CSR @16:22:20.43 Pulling 5ml of CSR.flush @16:22:20.44 CSR.select :flush @16:22:20.55 CS.seek 5ml @16:22:34.13 delay 5 seconds @16:22:39.15 CSR.select :air @16:22:39.30 CS.reconfigure :fast @16:22:39.34 CS.seek 7.501ml @16:22:46.00 CSV.dial :waste @16:22:47.26 CS.reconfigure :default @16:22:47.30 CS.seek 0ml @16:23:07.47 Flushing lower manifold's waste1 port @16:23:07.58 CSV.dial :flush @16:23:08.02 CS.reconfigure :fast @16:23:08.06 CS.seek 5ml @16:23:20.63 delay 5 seconds @16:23:25.64 CSV.dial :air @16:23:26.14 CS.seek 7ml @16:23:31.60 CSV.dial CTV @16:23:31.61
CTV.dial CSV @16:23:32.97 CBV.dial :waste1 @16:23:33.73 CS.seek 0ml @16:23:50.19 Flushing lower manifold's waste2 port @16:23:50.31 CSV.dial :flush @16:23:51.02 CS.seek 5ml @16:24:03.59 delay 5 seconds @16:24:08.61 CSV.dial :air @16:24:09.12 CS.seek 7.001ml @16:24:14.63 CSV.dial CTV @16:24:14.66
CTV.dial CSV @16:24:15.08 CBV.dial :waste2 @16:24:16.12 CS.seek 0ml @16:24:32.66 Flushing lower manifold's waste2b port @16:24:32.77 CSV.dial :flush @16:24:33.62 CS.seek 5ml @16:24:46.12 delay 5 seconds @16:24:51.14 CSV.dial :air @16:24:51.64 CS.seek 7ml @16:24:57.19 CSV.dial CTV @16:24:57.21
CTV.dial CSV @16:24:57.66 CBV.dial :waste2b @16:24:58.69 CS.seek 0ml @16:25:15.22 Disinfecting external Intake with kill @16:25:15.34 CSV.dial CSR @16:25:15.82 Pulling 8ml of CSR.kill @16:25:15.83 CSR.select :kill @16:25:15.94 CS.seek 8ml @16:25:35.75 delay 5 seconds @16:25:40.77 CSR.select :air @16:25:40.88 CS.seek 10ml @16:25:46.53 CSV.dial CBV @16:25:46.55
CBV.dial CSV @16:25:47.57 CTV.dial :intake @16:25:48.33 Intake.select :open @16:25:48.43 CS.seek 2ml @16:26:07.21 Intake.select :close @16:26:07.31 CTV.dial :air @16:26:08.06 CS.reconfigure :default @16:26:08.11 CS.seek 0ml @16:26:14.25 CSV.dial CSR @16:26:15.28 Pulling 5ml of CSR.flush @16:26:15.29 CSR.select :flush @16:26:15.41 CS.seek 5ml @16:26:28.97 delay 5 seconds @16:26:33.98 CSR.select :air @16:26:34.58 CS.seek 10ml @16:26:48.15 CSV.dial CBV @16:26:49.16 CS.seek 0ml @16:27:15.53 Garaging FlushPuck in Collection Clamp @16:27:15.60 Elbow.reconfigure :tight @16:27:15.64 Elbow.seek CC @16:27:15.96 Elbow.reconfigure :loose @16:27:16.05 Forearm.seek CC @16:27:17.01 Hand.close @16:27:17.68 CC.seek :open @16:27:22.29 Forearm.seek :retract @16:27:23.27 Elbow.reconfigure :tight @16:27:23.31 Elbow.seek :garage @16:27:26.13 Elbow.reconfigure :loose @16:27:26.22 Forearm.reconfigure :tight @16:27:26.26 Forearm.seek :garage @16:27:27.17 Hand.open @16:27:27.86 Forearm.reconfigure :loose @16:27:27.90 Forearm.seek :retract @16:27:28.85 Completed [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] begun 13:37:59 @16:27:28.89 Asleep after completing DA_WCR_120622 assays @16:27:28.91 GoodNews.email "{1 => 24,\n 2 => 9,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@16:27:09, 16% flow, 23.0C, 27% humidity, 15.0psia, 13.744V, 0.441A, 0.423A avg, 6.07W\nSampled 1000.0ml",Subject: "Asleep after completing DA_WCR_120622 assays" @16:27:34.93 upsync @15:17:36.88UTC13-Dec-22
ESP2 v3.41 I2C Gateway v3.20 for core bus @15:17:39.48 Configuration for short puck jake 1/11/21 by brent@mbari.org /home/esp/esp2/type/shallow/jake/configure.rb @15:17:43.47 Polling Can every 10:00 @15:17:43.53 Cleanly exited at 15:14:29UTC13-Dec-22 @15:17:43.57 Loading /home/esp/esp2/mission/DA_WCR_121322.rb mission @15:17:43.63 GoodNews.email "Starting DA_WCR_121322 w/pucks in tube 2\n{1 => 24,\n 2 => 9,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@15:17:43, 17% flow, 16.1C, 27% humidity, 14.6psia, 13.783V, 0.266A, 0.248A avg, 3.67W",Subject: "DA_WCR_121322 begun" @15:17:43.67
Preparing to sleep until + 00:00:05 @15:17:43.69 Asleep til + 00:00:05 @15:17:43.71 Continuing at 15:17 @15:17:44.00 Gate.power :core,:OFF @15:17:44.01 Gate.power :raw,:OFF @15:17:44.02 Shutdown @15:17:48.56 GoodNews.email "Then, in tube 2: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2/mission/DA_WCR_121322.rb:14\n{1 => 24,\n 2 => 9,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@15:17:43, 17% flow, 16.1C, 27% humidity, 14.6psia, 13.783V, 0.266A, 0.248A avg, 3.67W",Subject: "Asleep until 15:17" @15:17:48.75
Powering core bus @15:17:50.51 upsync @15:17:54.24
Gate.power :raw,:ON @15:17:54.29 upsync @15:17:55.17
CS.configure :default @15:17:55.26 PS.configure :default @15:17:55.35 SS.configure :default @15:17:55.43 CC.configure :default @15:17:55.52 PC.configure :default @15:17:55.63 Forearm.configure :loose @15:17:55.72 Elbow.configure :loose @15:17:55.82 SC.configure :loose @15:17:55.91 SE.configure :default @15:17:55.95 CH.coast @15:17:56.00 CH.configure :default @15:17:56.03 PH.coast @15:17:56.08 PH.configure :default @15:17:56.11 All dwarf controllers running firmware version 3.85 @15:17:56.22 Valve.reset @15:17:56.45 Intake.select :close @15:17:56.50 Exhaust.select :close @15:17:56.51 CTV.dial :air @15:17:56.59 PSV.dial :air @15:17:56.61 CSV.dial :waste1 @15:17:56.75
Forearm.reconfigure :loose @15:17:56.79 Forearm.home.seek :retract @15:17:57.12 SSV.dial :spit,avoiding: :exhaust @15:17:57.50 Hand.close @15:17:57.52
Elbow.jog 1500 @15:17:57.72 PS.reconfigure :default @15:17:57.76 PS.home.seek 0ml @15:17:57.94 CS.reconfigure :fast @15:17:57.97 SS.reconfigure :default @15:17:58.05 CS.home.seek 0ml @15:17:58.08 SS.home.seek 0ml @15:17:58.20
Elbow.reconfigure :loose @15:17:58.24 Elbow.home.seek :clear @15:18:02.76 SE.reconfigure :default @15:18:02.80 SE.home.seek :down @15:18:03.40 SC.reconfigure :tight @15:18:03.44 SC.home.seek :initial @15:18:06.01 PS.stop @15:18:31.74
SC.configure :limp @15:18:31.79 Forearm.seek :retract @15:18:31.84 Elbow.seek :hammer @15:18:32.87 Forearm.reconfigure :tight @15:18:32.91 Forearm.seek retracted @15:18:33.16 PC.reconfigure :default @15:18:33.18
CC.reconfigure :default @15:18:33.26 PC.home.seek :open @15:18:33.29
CC.home.seek :open @15:18:36.79 Phase [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] @15:18:36.80 Notice.email "At 15:18:36 began in tube 2: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2/mission/DA_WCR_121322.rb:14\n{1 => 24,\n 2 => 9,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@15:18:36, 18% flow, 16.1C, 27% humidity, 14.6psia, 13.769V, 0.315A, 0.302A avg, 4.33W",Subject: "Started DA_WCR_121322 phase initialPurge; da [1000, 1000] #da and wsr are run simultanelously" @15:18:36.82
Starting initialPurge @15:18:36.84 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @15:18:36.91 Hand.open @15:18:37.68 Elbow.reconfigure :tight @15:18:37.72 Elbow.seek FlushPuck::Garage @15:18:39.66 Elbow.reconfigure :loose @15:18:39.75 Forearm.reconfigure :loose @15:18:39.79 Forearm.seek :garage @15:18:40.77 Hand.close @15:18:41.58 Forearm.seek :retract @15:18:42.31 upsync @15:18:42.63
Elbow.reconfigure :tight @15:18:42.67 Elbow.seek CC @15:18:45.58 Elbow.reconfigure :loose @15:18:45.68 Forearm.reconfigure :tight @15:18:45.72 Forearm.seek CC @15:18:46.92 CC.seek :closed @15:18:51.47 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.26 @15:18:51.55 Hand.open @15:18:52.23 Forearm.reconfigure :loose @15:18:52.27 Forearm.seek :retract @15:18:53.23 Flushing the Collection Valve::Manifold @15:18:53.35 CSV.dial CSR @15:18:54.67 Pulling 5ml of CSR.flush @15:18:54.68 CSR.select :flush @15:18:54.79 CS.reconfigure :default @15:18:54.83 CS.seek 5ml @15:19:08.66 delay 5 seconds @15:19:13.68 CSR.select :air @15:19:13.83 CS.reconfigure :fast @15:19:13.87 CS.seek 7.499ml @15:19:20.80 CSV.dial :waste @15:19:22.07 CS.reconfigure :default @15:19:22.12 CS.seek 0ml @15:19:42.18 Purging external Intake @15:19:42.29 CSV.dial :flush @15:19:42.86 CS.reconfigure :fast @15:19:42.90 CS.seek 10ml @15:20:07.24 delay 5 seconds @15:20:12.26 CSV.dial CBV @15:20:12.29
CBV.dial CSV @15:20:13.02 CTV.dial :intake @15:20:13.82 Intake.select :open @15:20:13.92 CS.seek 0ml @15:20:37.43 Intake.select :close @15:20:37.53 CTV.dial :air @15:20:38.32 Flushing upper manifold @15:20:38.43 CSV.dial :flush @15:20:39.17 CS.seek 5ml @15:20:51.74 delay 5 seconds @15:20:56.76 CSV.dial :air @15:20:57.38 CS.seek 7ml @15:21:02.86 CSV.dial CBV @15:21:02.94
CBV.dial CSV @15:21:03.93 CTV.dial :air @15:21:03.95 CS.seek 0ml @15:21:20.52 delay 7 seconds @15:21:27.55 CS.seek 3ml @15:21:35.49 CSV.dial :waste @15:21:35.94 CS.seek 0ml @15:21:43.78 CSV.dial :flush @15:21:44.21 CS.seek 3ml @15:21:52.21 delay 5 seconds @15:21:57.23 CSV.dial :air @15:21:57.85 CS.seek 5ml @15:22:03.49 CSV.dial :air @15:22:03.51 CS.seek 0ml @15:22:15.83 delay 7 seconds @15:22:22.86 CS.seek 3ml @15:22:30.74 CSV.dial :waste @15:22:31.46 CS.seek 0ml @15:22:39.15 Garaging FlushPuck in Collection Clamp @15:22:39.21 Elbow.reconfigure :tight @15:22:39.25 Elbow.seek CC @15:22:39.59 Elbow.reconfigure :loose @15:22:39.68 Forearm.seek CC @15:22:40.65 Hand.close @15:22:41.29 CC.seek :open @15:22:45.94 Forearm.seek :retract @15:22:46.95 Elbow.reconfigure :tight @15:22:46.99 Elbow.seek :garage @15:22:49.95 Elbow.reconfigure :loose @15:22:50.04 Forearm.reconfigure :tight @15:22:50.08 Forearm.seek :garage @15:22:51.09 Hand.open @15:22:52.22 Forearm.reconfigure :loose @15:22:52.26 Forearm.seek :retract @15:22:53.25 da sampling at most 1000ml, wcr at most 1000ml @15:22:53.28 DAfans.email "Started da in tube 2 at 15:22:53UTC13-Dec-22\n{1 => 24,\n 2 => 9,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@15:18:36, 18% flow, 16.1C, 27% humidity, 14.6psia, 13.769V, 0.315A, 0.302A avg, 4.33W",Subject: "da sampling at most 1000ml, wcr at most 1000ml" @15:22:53.30
Loading sh1 puck from tube 2 into Collection Clamp @15:22:53.55 SC.reconfigure :tight @15:22:53.59 SC.seek 2 @15:22:58.77 upsync @15:23:00.35
SC.configure :limp @15:23:00.56 Elbow.reconfigure :tight @15:23:00.60 Elbow.seek 2 @15:23:02.66 Forearm.reconfigure :tight @15:23:02.70 Forearm.seek 2 + 450 counts @15:23:03.79 SE.reconfigure :pick @15:23:03.84 SE.seek :beamBreak @15:23:06.46 SE.configure :default @15:23:06.60 Elbow.seek 2 @15:23:07.14 Forearm.seek 2 + 300 counts @15:23:07.30 Forearm.reconfigure :loose @15:23:07.35 Hand.close @15:23:07.93 SE.seek :down @15:23:08.11
Forearm.seek :retract @15:23:09.06 Elbow.seek CC @15:23:10.19 SE.stop @15:23:10.78
Elbow.reconfigure :loose @15:23:10.88 Forearm.reconfigure :tight @15:23:10.92 Forearm.seek CC @15:23:12.18 CC.seek :closed @15:23:16.69 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.16 @15:23:16.70 Hand.open @15:23:17.35 Forearm.reconfigure :loose @15:23:17.39 Forearm.seek :retract @15:23:18.35 collectSample 1000ml w/bubble point of 24psi @15:23:18.37 Priming 7ml @15:23:18.38 Intake.select :close @15:23:18.39 Exhaust.select :close @15:23:18.40 SS.hold @15:23:18.60 Calibrating pressure and temperature sensors @15:23:18.63 CTV.dial :air @15:23:18.64 SSV.dial :spit,avoiding: :exhaust @15:23:18.66 SS.reconfigure :fast @15:23:18.70 SS.seek 5ml @15:23:21.45 SSV.dial CBV @15:23:21.50
CBV.dial SSV @15:23:22.80 delay 3.8 seconds @15:23:26.71 SSV.dial :spit @15:23:28.04 SS.seek 0ml @15:23:30.75 SSV.dial CBV @15:23:32.04 CTV.dial :intake @15:23:32.84 SSV.dial CBV @15:23:32.86
CBV.dial SSV @15:23:32.87 Allowing 40s for 7.0ml of remaining 7.0ml @15:23:32.88 Intake.select :open @15:23:35.03 Intake @15.4psia, vacuum from 9.9 to 12.9psi @15:23:35.05 SS.reconfigure :inhaling 2.20ml/s @15:23:35.09 SS.seek 7ml @15:23:42.43 Stroke completed -- waiting for pressure to equalize ... @15:23:42.44 SS.reconfigure :exhale @15:23:42.48 SS.hold @15:23:44.03 Intake.select :close @15:23:44.17 SSV.dial :exhaust @15:23:44.91 Exhaust.select :open @15:23:45.01 SS.seek 0ml @15:23:48.70 Exhaust.select :close @15:23:48.80 Sampling 1000.0ml @15:23:48.81 Intake.select :close @15:23:48.82 Exhaust.select :close @15:23:48.84 SS.reconfigure :default @15:23:48.87 SS.hold @15:23:49.06 CTV.dial :intake @15:23:49.08 SSV.dial CBV @15:23:49.10
CBV.dial SSV @15:23:49.86 Allowing 130s for 25.0ml of remaining 1000.0ml @15:23:49.87 Intake.select :open @15:23:52.02 Intake @15.2psia, vacuum from 9.7 to 12.7psi @15:23:52.04 SS.reconfigure :inhaling 2.20ml/s @15:23:52.08 SS.seek 25ml @15:24:02.36 Vacuum too high -- waiting up to 40 seconds @15:24:02.37 SS.reconfigure :exhale @15:24:02.42 SS.hold @15:24:03.10 Intake @11.4psia, vacuum from 5.9 to 8.9psi @15:24:03.12 SS.reconfigure :inhaling 1.98ml/s @15:24:03.16 SS.seek 25ml @15:24:06.99 Vacuum too high -- waiting up to 40 seconds @15:24:07.00 SS.reconfigure :exhale @15:24:07.04 SS.hold @15:24:12.97 Intake @14.0psia, vacuum from 8.5 to 11.5psi @15:24:12.99 SS.reconfigure :inhaling 1.79ml/s @15:24:13.03 SS.seek 25ml @15:24:16.06 Stroke completed -- waiting for pressure to equalize ... @15:24:16.07 SS.reconfigure :exhale @15:24:16.11 SS.hold @15:24:17.65 Intake.select :close @15:24:17.79 SSV.dial :exhaust @15:24:18.53 Exhaust.select :open @15:24:18.64 SS.seek 0ml @15:24:27.20 Exhaust.select :close @15:24:27.30 SSV.dial CBV @15:24:27.32
CBV.dial SSV @15:24:28.08 Allowing 130s for 25.0ml of remaining 975.0ml @15:24:28.09 Intake.select :open @15:24:30.24 Intake @15.3psia, vacuum from 9.8 to 12.8psi @15:24:30.25 SS.reconfigure :inhaling 1.79ml/s @15:24:30.30 SS.seek 25ml @15:24:45.33 Vacuum too high -- waiting up to 40 seconds @15:24:45.34 SS.reconfigure :exhale @15:24:45.38 SS.hold @15:24:45.96 Intake @11.9psia, vacuum from 6.4 to 9.4psi @15:24:45.97 SS.reconfigure :inhaling 1.61ml/s @15:24:46.02 SS.seek 25ml @15:24:49.12 Stroke completed -- waiting for pressure to equalize ... @15:24:49.13 SS.reconfigure :exhale @15:24:49.17 SS.hold @15:24:55.57 Intake.select :close @15:24:55.71 SSV.dial :exhaust @15:24:56.45 Exhaust.select :open @15:24:56.56 SS.seek 0ml @15:25:05.48 Exhaust.select :close @15:25:05.58 SSV.dial CBV @15:25:05.60
CBV.dial SSV @15:25:06.36 Allowing 130s for 25.0ml of remaining 950.0ml @15:25:06.37 Intake.select :open @15:25:08.52 Intake @15.3psia, vacuum from 9.8 to 12.8psi @15:25:08.54 SS.reconfigure :inhaling 1.61ml/s @15:25:08.58 SS.seek 25ml @15:25:26.81 Stroke completed -- waiting for pressure to equalize ... @15:25:26.82 SS.reconfigure :exhale @15:25:26.86 SS.hold @15:25:35.32 Intake.select :close @15:25:35.46 SSV.dial :exhaust @15:25:36.20 Exhaust.select :open @15:25:36.30 SS.seek 0ml @15:25:45.04 Exhaust.select :close @15:25:45.15 SSV.dial CBV @15:25:45.18
CBV.dial SSV @15:25:45.92 Allowing 130s for 25.0ml of remaining 925.0ml @15:25:45.93 Intake.select :open @15:25:48.08 Intake @15.3psia, vacuum from 9.8 to 12.8psi @15:25:48.10 SS.reconfigure :inhaling 1.61ml/s @15:25:48.14 SS.seek 25ml @15:26:06.93 Vacuum too high -- waiting up to 40 seconds @15:26:06.94 SS.reconfigure :exhale @15:26:06.98 SS.hold @15:26:07.26 Intake @12.1psia, vacuum from 6.6 to 9.6psi @15:26:07.28 SS.reconfigure :inhaling 1.45ml/s @15:26:07.32 SS.seek 25ml @15:26:07.37 Stroke completed -- waiting for pressure to equalize ... @15:26:07.38 SS.reconfigure :exhale @15:26:07.42 SS.hold @15:26:14.79 Intake.select :close @15:26:14.93 SSV.dial :exhaust @15:26:15.67 Exhaust.select :open @15:26:15.77 SS.seek 0ml @15:26:24.56 Exhaust.select :close @15:26:24.66 SSV.dial CBV @15:26:24.68
CBV.dial SSV @15:26:25.43 Allowing 130s for 25.0ml of remaining 900.0ml @15:26:25.44 Intake.select :open @15:26:27.60 Intake @15.4psia, vacuum from 9.9 to 12.9psi @15:26:27.61 SS.reconfigure :inhaling 1.45ml/s @15:26:27.66 SS.seek 25ml @15:26:48.06 Stroke completed -- waiting for pressure to equalize ... SS.reconfigure :exhale @15:26:48.10 SS.hold @15:26:55.01 Intake.select :close @15:26:55.15 SSV.dial :exhaust @15:26:55.89 Exhaust.select :open @15:26:55.99 SS.seek 0ml @15:27:04.71 Exhaust.select :close @15:27:04.81 SSV.dial CBV @15:27:04.84
CBV.dial SSV @15:27:05.59 Allowing 130s for 25.0ml of remaining 875.0ml @15:27:05.60 Intake.select :open @15:27:07.75 Intake @15.3psia, vacuum from 9.8 to 12.8psi @15:27:07.77 SS.reconfigure :inhaling 1.45ml/s @15:27:07.81 SS.seek 25ml @15:27:28.12 Stroke completed -- waiting for pressure to equalize ... @15:27:28.13 SS.reconfigure :exhale @15:27:28.17 SS.hold @15:27:35.65 Intake.select :close @15:27:35.78 SSV.dial :exhaust @15:27:36.52 Exhaust.select :open @15:27:36.63 SS.seek 0ml @15:27:45.57 Exhaust.select :close @15:27:45.67 SSV.dial CBV @15:27:45.69
CBV.dial SSV @15:27:46.45 Allowing 130s for 25.0ml of remaining 850.0ml @15:27:46.46 Intake.select :open @15:27:48.61 Intake @15.4psia, vacuum from 9.9 to 12.9psi @15:27:48.63 SS.reconfigure :inhaling 1.45ml/s @15:27:48.67 SS.seek 25ml @15:28:08.51 Stroke completed -- waiting for pressure to equalize ... @15:28:08.52 SS.reconfigure :exhale @15:28:08.56 SS.hold @15:28:17.17 Intake.select :close @15:28:17.30 SSV.dial :exhaust @15:28:18.04 Exhaust.select :open @15:28:18.15 SS.seek 0ml @15:28:26.93 Exhaust.select :close @15:28:27.03 SSV.dial CBV @15:28:27.06
CBV.dial SSV @15:28:27.81 Allowing 130s for 25.0ml of remaining 825.0ml @15:28:27.82 Intake.select :open @15:28:29.97 Intake @15.3psia, vacuum from 9.8 to 12.8psi @15:28:29.98 SS.reconfigure :inhaling 1.45ml/s @15:28:30.02 SS.seek 25ml @15:28:46.79 Vacuum too high -- waiting up to 40 seconds @15:28:46.80 SS.reconfigure :exhale @15:28:46.85 SS.hold @15:28:47.33 Intake @12.5psia, vacuum from 7.0 to 10.0psi @15:28:47.34 SS.reconfigure :inhaling 1.30ml/s @15:28:47.39 SS.seek 25ml @15:28:51.86 Stroke completed -- waiting for pressure to equalize ... @15:28:51.87 SS.reconfigure :exhale @15:28:51.91 SS.hold @15:28:59.28 Intake.select :close @15:28:59.42 SSV.dial :exhaust @15:29:00.16 Exhaust.select :open @15:29:00.27 SS.seek 0ml @15:29:09.05 Exhaust.select :close @15:29:09.16 SSV.dial CBV @15:29:09.18
CBV.dial SSV @15:29:09.93 Allowing 130s for 25.0ml of remaining 800.0ml @15:29:09.94 Intake.select :open @15:29:12.09 Intake @15.3psia, vacuum from 9.8 to 12.8psi @15:29:12.11 SS.reconfigure :inhaling 1.30ml/s @15:29:12.15 SS.seek 25ml @15:29:34.37 Stroke completed -- waiting for pressure to equalize ... SS.reconfigure :exhale @15:29:34.41 SS.hold @15:29:41.78 Intake.select :close @15:29:41.91 SSV.dial :exhaust @15:29:42.65 Exhaust.select :open @15:29:42.75 SS.seek 0ml @15:29:51.54 Exhaust.select :close @15:29:51.64 SSV.dial CBV @15:29:52.15
CBV.dial SSV @15:29:52.91 Allowing 130s for 25.0ml of remaining 775.0ml @15:29:52.92 Intake.select :open @15:29:55.08 Intake @15.3psia, vacuum from 9.8 to 12.8psi @15:29:55.10 SS.reconfigure :inhaling 1.30ml/s @15:29:55.14 SS.seek 25ml @15:30:17.40 Stroke completed -- waiting for pressure to equalize ... @15:30:17.41 SS.reconfigure :exhale @15:30:17.45 SS.hold @15:30:24.89 Intake.select :close @15:30:25.03 SSV.dial :exhaust @15:30:25.77 Exhaust.select :open @15:30:25.88 SS.seek 0ml @15:30:34.69 Exhaust.select :close @15:30:34.79 SSV.dial CBV @15:30:34.82
CBV.dial SSV @15:30:35.59 Allowing 130s for 25.0ml of remaining 750.0ml Intake.select :open @15:30:37.74 Intake @15.3psia, vacuum from 9.8 to 12.8psi @15:30:37.76 SS.reconfigure :inhaling 1.30ml/s @15:30:37.80 SS.seek 25ml @15:30:59.89 Stroke completed -- waiting for pressure to equalize ... @15:30:59.90 SS.reconfigure :exhale @15:30:59.94 SS.hold @15:31:08.39 Intake.select :close @15:31:08.55 SSV.dial :exhaust @15:31:09.30 Exhaust.select :open @15:31:09.41 SS.seek 0ml @15:31:18.21 Exhaust.select :close @15:31:18.31 SSV.dial CBV @15:31:18.33
CBV.dial SSV @15:31:19.10 Allowing 130s for 25.0ml of remaining 725.0ml @15:31:19.11 Intake.select :open @15:31:21.26 Intake @15.4psia, vacuum from 9.9 to 12.9psi @15:31:21.28 SS.reconfigure :inhaling 1.30ml/s @15:31:21.32 SS.seek 25ml @15:31:43.50 Stroke completed -- waiting for pressure to equalize ... @15:31:43.51 SS.reconfigure :exhale @15:31:43.55 SS.hold @15:31:51.99 Intake.select :close @15:31:52.13 SSV.dial :exhaust @15:31:52.88 Exhaust.select :open @15:31:52.98 SS.seek 0ml @15:32:02.02 Exhaust.select :close @15:32:02.12 SSV.dial CBV @15:32:02.14
CBV.dial SSV @15:32:02.91 Allowing 130s for 25.0ml of remaining 700.0ml @15:32:02.92 Intake.select :open @15:32:05.07 Intake @15.3psia, vacuum from 9.8 to 12.8psi @15:32:05.09 SS.reconfigure :inhaling 1.30ml/s @15:32:05.13 SS.seek 25ml @15:32:26.88 Stroke completed -- waiting for pressure to equalize ... @15:32:26.89 SS.reconfigure :exhale @15:32:26.93 SS.hold @15:32:35.37 Intake.select :close @15:32:35.51 SSV.dial :exhaust @15:32:36.27 Exhaust.select :open @15:32:36.38 SS.seek 0ml @15:32:45.36 Exhaust.select :close @15:32:45.46 SSV.dial CBV @15:32:45.49
CBV.dial SSV @15:32:46.25 Allowing 130s for 25.0ml of remaining 675.0ml @15:32:46.26 Intake.select :open @15:32:48.42 Intake @15.4psia, vacuum from 9.9 to 12.9psi @15:32:48.43 SS.reconfigure :inhaling 1.30ml/s @15:32:48.48 SS.seek 25ml @15:33:10.25 Stroke completed -- waiting for pressure to equalize ... @15:33:10.26 SS.reconfigure :exhale @15:33:10.30 SS.hold @15:33:19.23 Intake.select :close @15:33:19.37 SSV.dial :exhaust @15:33:20.13 Exhaust.select :open @15:33:20.24 SS.seek 0ml @15:33:28.99 Exhaust.select :close @15:33:29.09 SSV.dial CBV @15:33:29.11
CBV.dial SSV @15:33:29.89 Allowing 130s for 25.0ml of remaining 650.0ml @15:33:29.90 Intake.select :open @15:33:32.06 Intake @15.3psia, vacuum from 9.8 to 12.8psi @15:33:32.08 SS.reconfigure :inhaling 1.30ml/s @15:33:32.12 SS.seek 25ml @15:33:51.32 Vacuum too high -- waiting up to 40 seconds @15:33:51.33 SS.reconfigure :exhale @15:33:51.37 SS.hold @15:33:53.46 Intake @12.9psia, vacuum from 7.4 to 10.4psi @15:33:53.48 SS.reconfigure :inhaling 1.17ml/s @15:33:53.52 SS.seek 25ml @15:33:57.73 Stroke completed -- waiting for pressure to equalize ... @15:33:57.74 SS.reconfigure :exhale @15:33:57.78 SS.hold @15:34:04.19 Intake.select :close @15:34:04.33 SSV.dial :exhaust @15:34:05.06 Exhaust.select :open @15:34:05.17 SS.seek 0ml @15:34:13.92 Exhaust.select :close @15:34:14.02 SSV.dial CBV @15:34:14.05
CBV.dial SSV @15:34:14.80 Allowing 130s for 25.0ml of remaining 625.0ml @15:34:14.81 Intake.select :open @15:34:16.96 Intake @15.3psia, vacuum from 9.8 to 12.8psi @15:34:16.98 SS.reconfigure :inhaling 1.17ml/s @15:34:17.02 SS.seek 25ml @15:34:41.34 Stroke completed -- waiting for pressure to equalize ... @15:34:41.35 SS.reconfigure :exhale @15:34:41.39 SS.hold @15:34:49.84 Intake.select :close @15:34:49.98 SSV.dial :exhaust @15:34:50.72 Exhaust.select :open @15:34:50.83 SS.seek 0ml @15:34:59.62 Exhaust.select :close @15:34:59.73 SSV.dial CBV @15:34:59.78
CBV.dial SSV @15:35:00.49 Allowing 130s for 25.0ml of remaining 600.0ml @15:35:00.50 Intake.select :open @15:35:02.65 Intake @15.3psia, vacuum from 9.8 to 12.8psi @15:35:02.66 SS.reconfigure :inhaling 1.17ml/s @15:35:02.70 SS.seek 25ml @15:35:26.83 Stroke completed -- waiting for pressure to equalize ... @15:35:26.84 SS.reconfigure :exhale @15:35:26.88 SS.hold @15:35:36.54 Intake.select :close @15:35:36.68 SSV.dial :exhaust @15:35:37.42 Exhaust.select :open @15:35:37.53 SS.seek 0ml @15:35:46.34 Exhaust.select :close @15:35:46.44 SSV.dial CBV @15:35:46.47
CBV.dial SSV @15:35:47.24 Allowing 130s for 25.0ml of remaining 575.0ml @15:35:47.25 Intake.select :open @15:35:49.40 Intake @15.3psia, vacuum from 9.8 to 12.8psi @15:35:49.41 SS.reconfigure :inhaling 1.17ml/s @15:35:49.46 SS.seek 25ml @15:36:12.88 Vacuum too high -- waiting up to 40 seconds @15:36:12.89 SS.reconfigure :exhale @15:36:12.93 SS.hold @15:36:15.93 Intake @13.2psia, vacuum from 7.7 to 10.7psi @15:36:15.94 SS.reconfigure :inhaling 1.06ml/s @15:36:15.98 SS.seek 25ml @15:36:17.00 Stroke completed -- waiting for pressure to equalize ... @15:36:17.01 SS.reconfigure :exhale @15:36:17.05 SS.hold @15:36:23.85 Intake.select :close @15:36:23.99 SSV.dial :exhaust @15:36:24.75 Exhaust.select :open @15:36:24.86 SS.seek 0ml @15:36:33.84 Exhaust.select :close @15:36:33.94 SSV.dial CBV @15:36:33.97
CBV.dial SSV @15:36:34.73 Allowing 130s for 25.0ml of remaining 550.0ml @15:36:34.74 Intake.select :open @15:36:36.90 Intake @15.4psia, vacuum from 9.9 to 12.9psi @15:36:36.92 SS.reconfigure :inhaling 1.06ml/s @15:36:36.96 SS.seek 25ml @15:37:03.01 Stroke completed -- waiting for pressure to equalize ... @15:37:03.02 SS.reconfigure :exhale @15:37:03.06 SS.hold @15:37:12.72 Intake.select :close @15:37:12.86 SSV.dial :exhaust @15:37:13.61 Exhaust.select :open @15:37:13.72 SS.seek 0ml @15:37:22.76 Exhaust.select :close @15:37:22.87 SSV.dial CBV @15:37:22.91
CBV.dial SSV @15:37:23.64 Allowing 130s for 25.0ml of remaining 525.0ml @15:37:23.65 Intake.select :open @15:37:25.80 Intake @15.4psia, vacuum from 9.9 to 12.9psi @15:37:25.82 SS.reconfigure :inhaling 1.06ml/s @15:37:25.86 SS.seek 25ml @15:37:51.82 Stroke completed -- waiting for pressure to equalize ... @15:37:51.83 SS.reconfigure :exhale @15:37:51.87 SS.hold @15:38:01.53 Intake.select :close @15:38:01.66 SSV.dial :exhaust @15:38:02.43 Exhaust.select :open @15:38:02.53 SS.seek 0ml @15:38:11.54 Exhaust.select :close @15:38:11.65 SSV.dial CBV @15:38:11.69
CBV.dial SSV @15:38:12.42 Allowing 130s for 25.0ml of remaining 500.0ml @15:38:12.43 Intake.select :open @15:38:14.58 Intake @15.4psia, vacuum from 9.9 to 12.9psi @15:38:14.60 SS.reconfigure :inhaling 1.06ml/s @15:38:14.64 SS.seek 25ml @15:38:39.69 Vacuum too high -- waiting up to 40 seconds @15:38:39.70 SS.reconfigure :exhale @15:38:39.74 SS.hold @15:38:42.73 Intake @13.4psia, vacuum from 7.9 to 10.9psi @15:38:42.74 SS.reconfigure :inhaling 0.95ml/s @15:38:42.78 SS.seek 25ml @15:38:43.93 Stroke completed -- waiting for pressure to equalize ... @15:38:43.94 SS.reconfigure :exhale @15:38:43.98 SS.hold @15:38:50.42 Intake.select :close @15:38:51.02 SSV.dial :exhaust @15:38:51.79 Exhaust.select :open @15:38:51.90 SS.seek 0ml @15:39:00.70 Exhaust.select :close @15:39:00.80 SSV.dial CBV @15:39:00.82
CBV.dial SSV @15:39:01.59 Allowing 130s for 25.0ml of remaining 475.0ml @15:39:01.60 Intake.select :open @15:39:03.75 Intake @15.4psia, vacuum from 9.9 to 12.9psi @15:39:03.77 SS.reconfigure :inhaling 0.95ml/s @15:39:03.81 SS.seek 25ml @15:39:32.56 Stroke completed -- waiting for pressure to equalize ... @15:39:32.57 SS.reconfigure :exhale @15:39:32.61 SS.hold @15:39:42.39 Intake.select :close @15:39:42.53 SSV.dial :exhaust @15:39:43.28 Exhaust.select :open @15:39:43.39 SS.seek 0ml @15:39:52.40 Exhaust.select :close @15:39:52.51 SSV.dial CBV @15:39:52.53
CBV.dial SSV @15:39:53.29 Allowing 130s for 25.0ml of remaining 450.0ml @15:39:53.30 Intake.select :open @15:39:55.46 Intake @15.2psia, vacuum from 9.7 to 12.7psi @15:39:55.47 SS.reconfigure :inhaling 0.95ml/s @15:39:55.52 SS.seek 25ml @15:40:24.06 Stroke completed -- waiting for pressure to equalize ... @15:40:24.07 SS.reconfigure :exhale @15:40:24.11 SS.hold @15:40:33.85 Intake.select :close @15:40:33.98 SSV.dial :exhaust @15:40:34.73 Exhaust.select :open @15:40:34.84 SS.seek 0ml @15:40:43.63 Exhaust.select :close @15:40:43.75 SSV.dial CBV @15:40:43.77
CBV.dial SSV @15:40:44.53 Allowing 130s for 25.0ml of remaining 425.0ml @15:40:44.54 Intake.select :open @15:40:46.69 Intake @15.3psia, vacuum from 9.8 to 12.8psi @15:40:46.71 SS.reconfigure :inhaling 0.95ml/s @15:40:46.75 SS.seek 25ml @15:41:15.71 Stroke completed -- waiting for pressure to equalize ... @15:41:15.72 SS.reconfigure :exhale @15:41:15.76 SS.hold @15:41:26.80 Intake.select :close @15:41:26.94 SSV.dial :exhaust @15:41:27.68 Exhaust.select :open @15:41:27.79 SS.seek 0ml @15:41:36.74 Exhaust.select :close @15:41:36.85 SSV.dial CBV @15:41:36.87
CBV.dial SSV @15:41:37.62 Allowing 130s for 25.0ml of remaining 400.0ml @15:41:37.63 Intake.select :open @15:41:39.78 Intake @15.2psia, vacuum from 9.7 to 12.7psi @15:41:39.80 SS.reconfigure :inhaling 0.95ml/s @15:41:39.84 SS.seek 25ml @15:42:08.40 Stroke completed -- waiting for pressure to equalize ... @15:42:08.41 SS.reconfigure :exhale @15:42:08.45 SS.hold @15:42:19.47 Intake.select :close @15:42:19.62 SSV.dial :exhaust @15:42:20.37 Exhaust.select :open @15:42:20.48 SS.seek 0ml @15:42:29.27 Exhaust.select :close @15:42:29.38 SSV.dial CBV @15:42:29.41
CBV.dial SSV @15:42:30.13 Allowing 130s for 25.0ml of remaining 375.0ml @15:42:30.14 Intake.select :open @15:42:32.31 Intake @15.3psia, vacuum from 9.8 to 12.8psi @15:42:32.33 SS.reconfigure :inhaling 0.95ml/s @15:42:32.37 SS.seek 25ml @15:42:56.53 Vacuum too high -- waiting up to 40 seconds @15:42:56.54 SS.reconfigure :exhale @15:42:56.58 SS.hold @15:43:00.74 Intake @13.7psia, vacuum from 8.2 to 11.2psi @15:43:00.75 SS.reconfigure :inhaling 0.87ml/s @15:43:00.80 SS.seek 25ml @15:43:06.27 Stroke completed -- waiting for pressure to equalize ... @15:43:06.28 SS.reconfigure :exhale @15:43:06.32 SS.hold @15:43:13.69 Intake.select :close @15:43:13.82 SSV.dial :exhaust @15:43:14.57 Exhaust.select :open @15:43:14.68 SS.seek 0ml @15:43:23.49 Exhaust.select :close @15:43:23.59 SSV.dial CBV @15:43:23.62
CBV.dial SSV @15:43:24.37 Allowing 130s for 25.0ml of remaining 350.0ml @15:43:24.38 Intake.select :open @15:43:26.53 Intake @15.4psia, vacuum from 9.9 to 12.9psi @15:43:26.55 SS.reconfigure :inhaling 0.87ml/s @15:43:26.59 SS.seek 25ml @15:43:58.14 Stroke completed -- waiting for pressure to equalize ... @15:43:58.15 SS.reconfigure :exhale @15:43:58.19 SS.hold @15:44:09.23 Intake.select :close @15:44:09.36 SSV.dial :exhaust @15:44:10.10 Exhaust.select :open @15:44:10.20 SS.seek 0ml @15:44:19.03 Exhaust.select :close @15:44:19.14 SSV.dial CBV @15:44:19.16
CBV.dial SSV @15:44:19.93 Allowing 130s for 25.0ml of remaining 325.0ml @15:44:19.94 Intake.select :open @15:44:22.09 Intake @15.4psia, vacuum from 9.9 to 12.9psi @15:44:22.10 SS.reconfigure :inhaling 0.87ml/s @15:44:22.15 SS.seek 25ml @15:44:51.68 Vacuum too high -- waiting up to 40 seconds @15:44:51.69 SS.reconfigure :exhale @15:44:51.73 SS.hold @15:44:56.63 Intake @13.9psia, vacuum from 8.4 to 11.4psi @15:44:56.64 SS.reconfigure :inhaling 0.78ml/s @15:44:56.69 SS.seek 25ml @15:44:59.13 Stroke completed -- waiting for pressure to equalize ... @15:44:59.14 SS.reconfigure :exhale @15:44:59.18 SS.hold @15:45:05.75 Intake.select :close @15:45:05.89 SSV.dial :exhaust @15:45:06.64 Exhaust.select :open @15:45:06.75 SS.seek 0ml @15:45:15.78 Exhaust.select :close @15:45:15.89 SSV.dial CBV @15:45:15.91
CBV.dial SSV @15:45:16.68 Allowing 130s for 25.0ml of remaining 300.0ml @15:45:16.69 Intake.select :open @15:45:18.84 Intake @15.5psia, vacuum from 10.0 to 13.0psi @15:45:18.86 SS.reconfigure :inhaling 0.78ml/s @15:45:18.90 SS.seek 25ml @15:45:53.11 Stroke completed -- waiting for pressure to equalize ... @15:45:53.12 SS.reconfigure :exhale @15:45:53.16 SS.hold @15:46:04.36 Intake.select :close @15:46:04.50 SSV.dial :exhaust @15:46:05.25 Exhaust.select :open @15:46:05.36 SS.seek 0ml @15:46:14.17 Exhaust.select :close @15:46:14.28 SSV.dial CBV @15:46:14.33
CBV.dial SSV @15:46:15.05 Allowing 130s for 25.0ml of remaining 275.0ml @15:46:15.06 Intake.select :open @15:46:17.21 Intake @15.3psia, vacuum from 9.8 to 12.8psi @15:46:17.23 SS.reconfigure :inhaling 0.78ml/s @15:46:17.27 SS.seek 25ml @15:46:51.97 Stroke completed -- waiting for pressure to equalize ... @15:46:51.98 SS.reconfigure :exhale @15:46:52.02 SS.hold @15:47:03.04 Intake.select :close @15:47:03.17 SSV.dial :exhaust @15:47:03.92 Exhaust.select :open @15:47:04.03 SS.seek 0ml @15:47:12.81 Exhaust.select :close @15:47:12.92 SSV.dial CBV @15:47:12.94
CBV.dial SSV @15:47:13.71 Allowing 130s for 25.0ml of remaining 250.0ml @15:47:13.72 Intake.select :open @15:47:15.87 Intake @15.3psia, vacuum from 9.8 to 12.8psi @15:47:15.88 SS.reconfigure :inhaling 0.78ml/s @15:47:15.93 SS.seek 25ml @15:47:50.78 Stroke completed -- waiting for pressure to equalize ... @15:47:50.79 SS.reconfigure :exhale @15:47:50.83 SS.hold @15:48:03.48 Intake.select :close @15:48:03.61 SSV.dial :exhaust @15:48:04.38 Exhaust.select :open @15:48:04.48 SS.seek 0ml @15:48:13.34 Exhaust.select :close @15:48:13.45 SSV.dial CBV @15:48:13.50
CBV.dial SSV @15:48:14.22 Allowing 130s for 25.0ml of remaining 225.0ml @15:48:14.23 Intake.select :open @15:48:16.38 Intake @15.4psia, vacuum from 9.9 to 12.9psi @15:48:16.40 SS.reconfigure :inhaling 0.78ml/s @15:48:16.44 SS.seek 25ml @15:48:48.43 Vacuum too high -- waiting up to 40 seconds @15:48:48.44 SS.reconfigure :exhale @15:48:48.95 SS.hold @15:48:53.26 Intake @13.9psia, vacuum from 8.4 to 11.4psi @15:48:53.28 SS.reconfigure :inhaling 0.70ml/s @15:48:53.32 SS.seek 25ml @15:48:56.70 Stroke completed -- waiting for pressure to equalize ... @15:48:56.71 SS.reconfigure :exhale @15:48:56.75 SS.hold @15:49:04.12 Intake.select :close @15:49:04.25 SSV.dial :exhaust @15:49:05.00 Exhaust.select :open @15:49:05.11 SS.seek 0ml @15:49:13.90 Exhaust.select :close @15:49:14.01 SSV.dial CBV @15:49:14.03
CBV.dial SSV @15:49:14.78 Allowing 130s for 25.0ml of remaining 200.0ml @15:49:14.79 Intake.select :open @15:49:16.94 Intake @15.3psia, vacuum from 9.8 to 12.8psi @15:49:16.96 SS.reconfigure :inhaling 0.70ml/s @15:49:17.00 SS.seek 25ml @15:49:55.12 Stroke completed -- waiting for pressure to equalize ... @15:49:55.13 SS.reconfigure :exhale @15:49:55.17 SS.hold @15:50:07.72 Intake.select :close @15:50:07.86 SSV.dial :exhaust @15:50:08.59 Exhaust.select :open @15:50:08.70 SS.seek 0ml @15:50:17.48 Exhaust.select :close @15:50:17.58 SSV.dial CBV @15:50:17.61
CBV.dial SSV @15:50:18.36 Allowing 130s for 25.0ml of remaining 175.0ml @15:50:18.37 Intake.select :open @15:50:20.52 Intake @15.3psia, vacuum from 9.8 to 12.8psi @15:50:20.53 SS.reconfigure :inhaling 0.70ml/s @15:50:20.58 SS.seek 25ml @15:50:58.61 Stroke completed -- waiting for pressure to equalize ... @15:50:58.62 SS.reconfigure :exhale @15:50:58.66 SS.hold @15:51:11.30 Intake.select :close @15:51:11.43 SSV.dial :exhaust @15:51:12.17 Exhaust.select :open @15:51:12.28 SS.seek 0ml @15:51:21.03 Exhaust.select :close @15:51:21.13 SSV.dial CBV @15:51:21.15
CBV.dial SSV @15:51:21.90 Allowing 130s for 25.0ml of remaining 150.0ml @15:51:21.91 Intake.select :open @15:51:24.07 Intake @15.3psia, vacuum from 9.8 to 12.8psi @15:51:24.08 SS.reconfigure :inhaling 0.70ml/s @15:51:24.12 SS.seek 25ml @15:52:02.21 Stroke completed -- waiting for pressure to equalize ... @15:52:02.22 SS.reconfigure :exhale @15:52:02.26 SS.hold @15:52:16.61 Intake.select :close @15:52:16.75 SSV.dial :exhaust @15:52:17.50 Exhaust.select :open @15:52:17.60 SS.seek 0ml @15:52:26.35 Exhaust.select :close @15:52:26.46 SSV.dial CBV @15:52:26.49
CBV.dial SSV @15:52:27.22 Allowing 130s for 25.0ml of remaining 125.0ml @15:52:27.23 Intake.select :open @15:52:29.38 Intake @15.3psia, vacuum from 9.8 to 12.8psi @15:52:29.39 SS.reconfigure :inhaling 0.70ml/s @15:52:29.44 SS.seek 25ml @15:53:03.58 Vacuum too high -- waiting up to 40 seconds @15:53:03.59 SS.reconfigure :exhale @15:53:03.63 SS.hold @15:53:11.08 Intake @14.5psia, vacuum from 9.0 to 12.0psi @15:53:11.10 SS.reconfigure :inhaling 0.63ml/s @15:53:11.14 SS.seek 25ml @15:53:15.71 Stroke completed -- waiting for pressure to equalize ... @15:53:15.72 SS.reconfigure :exhale @15:53:15.76 SS.hold @15:53:24.21 Intake.select :close @15:53:24.35 SSV.dial :exhaust @15:53:25.09 Exhaust.select :open @15:53:25.19 SS.seek 0ml @15:53:33.99 Exhaust.select :close @15:53:34.10 SSV.dial CBV @15:53:34.15
CBV.dial SSV @15:53:34.85 Allowing 130s for 25.0ml of remaining 100.0ml @15:53:34.86 Intake.select :open @15:53:37.02 Intake @15.5psia, vacuum from 10.0 to 13.0psi @15:53:37.03 SS.reconfigure :inhaling 0.63ml/s @15:53:37.08 SS.seek 25ml @15:54:19.13 Stroke completed -- waiting for pressure to equalize ... @15:54:19.14 SS.reconfigure :exhale @15:54:19.18 SS.hold @15:54:33.44 Intake.select :close @15:54:33.58 SSV.dial :exhaust @15:54:34.32 Exhaust.select :open @15:54:34.43 SS.seek 0ml @15:54:43.10 Exhaust.select :close @15:54:43.21 SSV.dial CBV @15:54:43.25
CBV.dial SSV @15:54:43.96 Allowing 130s for 25.0ml of remaining 75.0ml @15:54:43.97 Intake.select :open @15:54:46.12 Intake @15.4psia, vacuum from 9.9 to 12.9psi @15:54:46.14 SS.reconfigure :inhaling 0.63ml/s @15:54:46.18 SS.seek 25ml @15:55:28.43 Stroke completed -- waiting for pressure to equalize ... @15:55:28.44 SS.reconfigure :exhale @15:55:28.48 SS.hold @15:55:43.20 Intake.select :close @15:55:43.34 SSV.dial :exhaust @15:55:44.09 Exhaust.select :open @15:55:44.19 SS.seek 0ml @15:55:52.96 Exhaust.select :close @15:55:53.06 SSV.dial CBV @15:55:53.08
CBV.dial SSV @15:55:53.84 Allowing 130s for 25.0ml of remaining 50.0ml @15:55:53.85 Intake.select :open @15:55:56.00 Intake @15.4psia, vacuum from 9.9 to 12.9psi @15:55:56.01 SS.reconfigure :inhaling 0.63ml/s @15:55:56.06 SS.seek 25ml @15:56:33.17 Vacuum too high -- waiting up to 40 seconds @15:56:33.18 SS.reconfigure :exhale @15:56:33.22 SS.hold @15:56:39.72 Intake @14.5psia, vacuum from 9.0 to 12.0psi @15:56:39.74 SS.reconfigure :inhaling 0.57ml/s @15:56:39.78 SS.seek 25ml @15:56:45.69 Stroke completed -- waiting for pressure to equalize ... @15:56:45.70 SS.reconfigure :exhale @15:56:45.74 SS.hold @15:56:56.77 Intake.select :close @15:56:56.90 SSV.dial :exhaust @15:56:57.65 Exhaust.select :open @15:56:57.75 SS.seek 0ml @15:57:06.55 Exhaust.select :close @15:57:06.65 SSV.dial CBV @15:57:06.67
CBV.dial SSV @15:57:07.43 Allowing 130s for 25.0ml of remaining 25.0ml @15:57:07.44 Intake.select :open @15:57:09.59 Intake @15.4psia, vacuum from 9.9 to 12.9psi @15:57:09.61 SS.reconfigure :inhaling 0.57ml/s @15:57:09.65 SS.seek 24.96ml @15:57:55.86 Stroke completed -- waiting for pressure to equalize ... @15:57:55.87 SS.reconfigure :exhale @15:57:55.91 SS.hold @15:58:12.10 Intake.select :close @15:58:12.24 SSV.dial :exhaust @15:58:12.97 Exhaust.select :open @15:58:13.08 SS.seek 0ml @15:58:21.92 Exhaust.select :close @15:58:22.03 Sampled 1000.0ml @15:58:22.04 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @15:58:22.05 Intake.select :close @15:58:22.10 CSV.dial :waste @15:58:22.15 CS.seek 0ml @15:58:22.25 SSV.dial CBV,avoiding: :exhaust @15:58:22.27 CSV.dial :air @15:58:22.31
CBV.dial SSV @15:58:23.04 CS.seek 10ml @15:58:24.18
CTV.dial :air @15:58:24.97 delay 15 seconds @15:58:39.99 SSV.dial :spit,avoiding: :exhaust @15:58:41.31 CTV.dial CSV,avoiding: :intake @15:58:41.36
SS.seek 0ml @15:58:41.92 SSV.dial CBV,avoiding: :exhaust @15:58:47.51 CBV.dial between SSV and waste2 @15:58:47.89 CTV.dial CSV,avoiding: :air @15:58:47.92
CSV.dial CTV @15:58:48.37 CS.reconfigure :pressurize @15:58:48.41 CS.seek 0ml @15:59:07.27 BubblePoint: Intake @38.9psia, Outlet @14.6psia @15:59:07.28 CBV.dial SSV @15:59:07.62 SS.reconfigure :default @15:59:07.66 SS.seek 10ml @15:59:13.25 delay 15 seconds @15:59:28.37 Evacuated: Intake @17.0psia, Outlet @9.5psia @15:59:28.38 SSV.dial :spit,avoiding: :exhaust @15:59:29.71 SS.reconfigure :exhale @15:59:29.76 SS.seek 0ml @15:59:35.03 SS.reconfigure :fast @15:59:35.07 SS.seek 5ml @15:59:37.93 SSV.dial CBV,avoiding: :exhaust @15:59:37.95
CBV.dial SSV @15:59:39.29 CTV.dial :air,avoiding: :intake @15:59:40.63 CSV.dial CTV @15:59:40.66
SS.seek 10ml @15:59:41.65 CSV.dial :air @15:59:42.10 CS.reconfigure :fast @15:59:42.14 CS.seek 0ml @15:59:43.13
SSV.dial :spit,avoiding: :exhaust @15:59:44.46 SS.reconfigure :exhale @15:59:44.51 SS.seek 0ml @15:59:49.46 SS.reconfigure :fast @15:59:49.47 CS.stop @15:59:49.55
SS.seek 5ml @15:59:52.50 SSV.dial CBV,avoiding: :exhaust @15:59:52.51
CBV.dial SSV @15:59:54.85 SSV.dial :spit,avoiding: :exhaust @15:59:56.16 SS.reconfigure :default @15:59:56.21 SS.seek 0ml @15:59:59.34 SS.stop @15:59:59.37 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @15:59:59.38 Intake.select :close @15:59:59.45 CSV.dial :waste @15:59:59.56 SSV.dial CBV,avoiding: :exhaust @15:59:59.58
CBV.dial SSV @16:00:00.24 CS.seek 0ml @16:00:00.31 CSV.dial :air @16:00:00.91
CTV.dial :air @16:00:00.92 delay 15 seconds @16:00:01.04 CS.seek 10ml @16:00:15.94
SSV.dial :spit,avoiding: :exhaust @16:00:17.26 CTV.dial CSV,avoiding: :intake @16:00:17.29
SS.reconfigure :exhale @16:00:17.38 SS.seek 0ml @16:00:17.78 SSV.dial CBV,avoiding: :exhaust @16:00:25.41 CBV.dial between SSV and waste2 @16:00:25.81 CTV.dial CSV,avoiding: :air @16:00:25.83
CSV.dial CTV @16:00:26.27 CS.reconfigure :pressurize @16:00:26.32 CS.seek 0ml @16:00:47.40 BubblePoint: Intake @37.6psia, Outlet @13.5psia CBV.dial SSV @16:00:47.72 SS.reconfigure :default @16:00:47.77 SS.seek 10ml @16:00:53.35 delay 15 seconds @16:01:08.46 Evacuated: Intake @18.3psia, Outlet @11.0psia @16:01:08.47 SSV.dial :spit,avoiding: :exhaust @16:01:09.79 SS.reconfigure :exhale @16:01:09.83 SS.seek 0ml @16:01:15.10 SS.reconfigure :fast @16:01:15.15 SS.seek 5ml @16:01:17.99 SSV.dial CBV,avoiding: :exhaust @16:01:18.01
CBV.dial SSV @16:01:19.33 CTV.dial :air,avoiding: :intake @16:01:20.67 CSV.dial CTV @16:01:20.70
SS.seek 10ml @16:01:21.71 CSV.dial :air @16:01:22.16 CS.reconfigure :fast @16:01:22.20 CS.seek 0ml @16:01:23.19
SSV.dial :spit,avoiding: :exhaust @16:01:24.51 SS.reconfigure :exhale @16:01:24.55 SS.seek 0ml @16:01:27.54 CS.stop @16:01:29.56
SS.reconfigure :fast @16:01:29.60 SS.seek 5ml @16:01:32.56 SSV.dial CBV,avoiding: :exhaust @16:01:32.58
CBV.dial SSV @16:01:34.90 SSV.dial :spit,avoiding: :exhaust @16:01:36.21 SS.reconfigure :default @16:01:36.25 SS.seek 0ml @16:01:39.78 SS.stop @16:01:39.82 Lysing: Adding 2.0ml AqMeOH @16:01:39.83 CBV.dial between CSV and waste1,avoiding: :DBV @16:01:41.33 CTV.dial CSV @16:01:42.64 CSV.dial CSR @16:01:43.43 Pulling 1ml of CSR.AqMeOH @16:01:43.44 CSR.select :AqMeOH @16:01:43.55 CS.reconfigure :default @16:01:43.59 CS.seek 1ml @16:01:47.17 delay 5 seconds @16:01:52.19 CSR.select :air @16:01:52.34 CS.seek 6ml @16:02:06.04 delay 2 seconds @16:02:08.06 CSV.dial :waste2 @16:02:08.49 CS.reconfigure :fast @16:02:08.53 CS.seek 0ml @16:02:22.92 CSV.dial CSR @16:02:23.41 Pulling 2ml of CSR.AqMeOH @16:02:23.42 CSR.select :AqMeOH @16:02:23.53 CS.reconfigure :default @16:02:23.57 CS.seek 2ml @16:02:29.48 delay 5 seconds @16:02:34.50 CSR.select :air @16:02:34.65 CS.seek 5ml @16:02:43.14 delay 2 seconds @16:02:45.15 CSV.dial CTV @16:02:45.58 CS.seek 1ml @16:02:56.89 Before lysing Collection Puck Heater at 15.9C/38.4psia @16:02:56.91 Heating Puck.from 2, :sh1 in Collection Clamp to 70C for 10:00 @16:02:56.93 CH.seek 70C,8:00 @16:04:56.25 CH.hold 10:00,70C @16:04:56.43 Begun lysing Collection Puck Heater at 68C/46.8psia @16:14:56.45 Ended lysing Collection Puck Heater at 70C/45.8psia @16:14:56.47 CS.seek 0ml @16:14:59.85 CTV.dial between spare and air,avoiding: :intake @16:15:00.88 CSV.dial :air @16:15:01.32 CS.seek 5ml @16:15:14.90 CSV.dial CTV @16:15:15.35 delay 2 seconds @16:15:17.36 CSV.dial :air @16:15:17.80 delay 2 seconds @16:15:19.81 CSV.dial CTV @16:15:20.27 delay 2 seconds @16:15:22.29 CSV.dial :air @16:15:22.73 delay 2 seconds @16:15:24.76 CS.seek 0ml @16:15:38.39 Puck @73.5psia after final lysis pressurization @16:15:38.40 Recovering Lysate into Collection syringe @16:15:38.41 CSV.dial CBV @16:15:39.88 CBV.dial CSV @16:15:40.26 CS.seek 5ml @16:15:54.03 delay 5 seconds @16:15:59.05 CTV.dial :air @16:15:59.57 CS.seek 7ml @16:16:05.57 delay 5 seconds @16:16:10.59 CSV.dial :air2 @16:16:11.04 CS.seek 10ml @16:16:19.59 CSV.dial between air and CTV,avoiding: CBV @16:16:20.75 Lysing Complete @16:16:20.82 Storing sh1 puck in Collection Clamp to tube 1 @16:16:20.98 SC.reconfigure :tight @16:16:21.02 SC.seek 1 @16:16:24.54 SC.configure :limp @16:16:24.73 Elbow.reconfigure :tight @16:16:24.78 Elbow.seek 1 @16:16:26.55 Forearm.reconfigure :tight @16:16:26.59 Forearm.seek 1 + 450 counts @16:16:27.62 SE.reconfigure :pick @16:16:27.67 SE.seek :beamBreak @16:16:29.59 SE.configure :default @16:16:29.74 Elbow.reconfigure :limp @16:16:29.78 Forearm.reconfigure :loose @16:16:29.82 Forearm.seek 1 @16:16:30.14 Elbow.hold @16:16:30.19 Elbow.reconfigure :tight @16:16:30.28 SE.seek 23.06 Pucks below Top Plate @16:16:30.77 Forearm.force -20 @16:16:30.80 Forearm.seek 1 + 236 counts @16:16:31.11 Forearm.reconfigure :tight @16:16:31.15 Forearm.seek 1 + 450 counts @16:16:31.44 SE.seek 24.06 Pucks below Top Plate @16:16:32.00 Forearm.reconfigure :loose @16:16:32.04 Forearm.seek :retract @16:16:32.98 Elbow.seek CC @16:16:34.66 Elbow.reconfigure :loose @16:16:34.75 Forearm.seek CC @16:16:35.71 Hand.close @16:16:36.37 CC.seek :open @16:16:41.08 Forearm.seek :retract @16:16:42.06 Elbow.reconfigure :tight @16:16:42.10 Elbow.seek 1 @16:16:43.79 Elbow.reconfigure :limp @16:16:43.83 Forearm.seek 1 @16:16:44.84 Elbow.hold @16:16:44.91 Elbow.reconfigure :tight @16:16:44.95 Hand.open @16:16:45.73 Forearm.force -20 @16:16:45.76 Forearm.seek 1 + 308 counts @16:16:46.04 Forearm.reconfigure :tight @16:16:46.08 Forearm.seek 1 + 450 counts @16:16:46.33 SE.seek :down @16:16:47.45
Forearm.reconfigure :loose @16:16:47.51 SE.stop @16:16:47.53
Forearm.seek :retract @16:16:48.50 Tranferring DA @16:16:48.51 Beginning samplePassC @16:16:48.53 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @16:16:48.70 Elbow.seek FlushPuck::Garage @16:16:50.52 Elbow.reconfigure :loose @16:16:50.61 Forearm.seek :garage @16:16:51.60 Hand.close @16:16:52.35 Forearm.seek :retract @16:16:53.31 Elbow.reconfigure :tight @16:16:53.35 Elbow.seek CC @16:16:56.30 Elbow.reconfigure :loose @16:16:56.39 Forearm.reconfigure :tight @16:16:56.43 Forearm.seek CC @16:16:58.16 CC.seek :closed @16:17:02.69 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.34 @16:17:02.75 Hand.open @16:17:03.43 Forearm.reconfigure :loose @16:17:03.47 Forearm.seek :retract @16:17:04.40 PSV.dial PSR @16:17:05.78 Pulling 1.5ml of PSR.flush @16:17:05.79 PSR.select :flush @16:17:05.90 PS.seek 1.5ml @16:17:22.01 delay 5 seconds @16:17:27.03 PSV.dial :waste1 @16:17:27.50 PS.seek 0ml @16:17:43.60 PSV.dial PSR @16:17:44.05 PSR.select :air @16:17:44.16 PS.seek 2.5ml @16:18:10.48 PRV.dial CBV,avoiding: :ab2 @16:18:10.52
CBV.dial PRV @16:18:12.18 PSV.dial :mixloop @16:18:13.19 CTV.dial :air,avoiding: :intake @16:18:13.21 PTV.dial :PRVmixing @16:18:14.53 PS.seek 0ml @16:18:41.03 PSV.dial :air @16:18:41.49 PS.seek 2.5ml @16:19:07.85 PSV.dial :mixloop @16:19:08.31 PS.seek 0ml @16:19:34.66 PSV.dial :air @16:19:35.12 PS.seek 2.5ml @16:20:01.44 PSV.dial :mixloop @16:20:01.90 PS.seek 0ml @16:20:28.28 Garaging FlushPuck in Collection Clamp @16:20:28.35 Elbow.reconfigure :tight @16:20:28.39 Elbow.seek CC @16:20:28.66 Elbow.reconfigure :loose @16:20:28.74 Forearm.seek CC @16:20:29.76 Hand.close @16:20:30.37 CC.seek :open @16:20:34.98 Forearm.seek :retract @16:20:35.96 Elbow.reconfigure :tight @16:20:36.01 Elbow.seek :garage @16:20:38.97 Elbow.reconfigure :loose @16:20:39.06 Forearm.reconfigure :tight @16:20:39.10 Forearm.seek :garage @16:20:40.18 Hand.open @16:20:40.80 Forearm.reconfigure :loose @16:20:40.84 Forearm.seek :retract @16:20:41.82 PSV.dial :mixloop @16:20:41.83 PTV.dial :PRVmixing @16:20:41.85 PRV.dial CBV @16:20:41.87
CBV.dial PRV @16:20:41.88 CTV.dial :air,avoiding: :intake @16:20:41.90 Loading lyfil puck from tube 2 into Collection Clamp @16:20:42.13 SC.reconfigure :tight @16:20:42.17 SC.seek 2 @16:20:45.72 SC.configure :limp @16:20:45.91 Elbow.reconfigure :tight @16:20:45.95 Elbow.seek 2 @16:20:47.92 Forearm.reconfigure :tight @16:20:47.96 Forearm.seek 2 + 450 counts @16:20:48.97 SE.reconfigure :pick @16:20:49.02 SE.seek :beamBreak @16:20:51.68 SE.configure :default @16:20:51.81 Elbow.seek 2 @16:20:51.98 Forearm.seek 2 + 300 counts @16:20:52.24 Forearm.reconfigure :loose @16:20:52.28 Hand.close @16:20:52.88 SE.seek :down @16:20:53.06
Forearm.seek :retract @16:20:54.43 Elbow.seek CC @16:20:55.20 SE.stop @16:20:56.07
Elbow.reconfigure :loose @16:20:56.16 Forearm.reconfigure :tight @16:20:56.20 Forearm.seek CC @16:20:57.39 CC.seek :closed @16:21:01.90 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.10 @16:21:01.91 Hand.open @16:21:02.56 Forearm.reconfigure :loose @16:21:02.60 Forearm.seek :retract @16:21:03.54 CTV.dial CSV,avoiding: :intake @16:21:04.90 CSV.dial CTV @16:21:05.22 PS.seek 2.5ml @16:21:31.69 CS.seek 4.5ml @16:21:46.59 delay 8 seconds @16:21:54.61 PSV.dial :air @16:21:55.08 delay 2 seconds @16:21:57.10 PSV.dial :waste2 @16:21:57.96 PS.seek 0ml @16:22:24.40 CBV.dial between PRV and waste1 @16:22:24.73 CSV.dial :air @16:22:25.19 CS.seek 10ml @16:22:40.02 CSV.dial CTV @16:22:40.47 CS.seek 0ml @16:23:07.08 CBV.dial :waste1 @16:23:07.38 delay 5 seconds @16:23:12.40 CTV.dial :air,avoiding: :intake @16:23:13.80 Storing lyfil puck in Collection Clamp to tube 1 @16:23:13.96 SC.reconfigure :tight @16:23:14.00 SC.seek 1 @16:23:17.62 SC.configure :limp @16:23:17.80 Elbow.reconfigure :tight @16:23:17.85 Elbow.seek 1 @16:23:19.63 Forearm.reconfigure :tight @16:23:19.68 Forearm.seek 1 + 450 counts @16:23:20.91 SE.reconfigure :pick @16:23:20.96 SE.seek :beamBreak @16:23:22.47 SE.configure :default @16:23:22.62 Elbow.reconfigure :limp @16:23:22.67 Forearm.reconfigure :loose @16:23:22.71 Forearm.seek 1 @16:23:22.90 Elbow.hold @16:23:22.96 Elbow.reconfigure :tight @16:23:23.04 SE.seek 24.06 Pucks below Top Plate @16:23:23.52 Forearm.force -20 @16:23:23.56 Forearm.seek 1 + 298 counts @16:23:23.85 Forearm.reconfigure :tight @16:23:23.90 Forearm.seek 1 + 450 counts @16:23:24.08 SE.seek 25.06 Pucks below Top Plate @16:23:24.68 Forearm.reconfigure :loose @16:23:24.73 Forearm.seek :retract @16:23:25.67 Elbow.seek CC @16:23:27.36 Elbow.reconfigure :loose @16:23:27.44 Forearm.seek CC @16:23:28.41 Hand.close @16:23:28.99 CC.seek :open @16:23:33.71 Forearm.seek :retract @16:23:34.68 Elbow.reconfigure :tight @16:23:34.72 Elbow.seek 1 @16:23:36.47 Elbow.reconfigure :limp @16:23:36.52 Forearm.seek 1 @16:23:37.49 Elbow.hold @16:23:38.02 Elbow.reconfigure :tight @16:23:38.07 Hand.open @16:23:38.73 Forearm.force -20 @16:23:38.77 Forearm.seek 1 + 294 counts @16:23:39.05 Forearm.reconfigure :tight @16:23:39.09 Forearm.seek 1 + 450 counts @16:23:39.34 SE.seek :down @16:23:40.34 SE.stop @16:23:40.49
Forearm.reconfigure :loose @16:23:40.54 Forearm.seek :retract @16:23:41.49 PSV.dial :air @16:23:42.37 PS.seek 2.5ml @16:24:08.81 delay 1 second @16:24:09.82 PSV.dial :mixloop @16:24:10.30 delay 1 second @16:24:11.31 PSV.dial :air @16:24:11.90 delay 1 second @16:24:12.92 PSV.dial :mixloop @16:24:13.36 delay 1 second @16:24:14.37 PSV.dial :air @16:24:14.83 delay 1 second @16:24:15.85 PS.seek 0ml @16:24:42.22 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @16:24:42.39 Elbow.seek FlushPuck::Garage @16:24:44.26 Elbow.reconfigure :loose @16:24:44.40 Forearm.seek :garage @16:24:45.35 Hand.close @16:24:46.10 Forearm.seek :retract @16:24:47.09 Elbow.reconfigure :tight @16:24:47.13 Elbow.seek PC @16:24:48.37 Elbow.reconfigure :loose @16:24:48.46 Forearm.reconfigure :tight @16:24:48.51 Forearm.seek PC @16:24:49.73 PC.seek :closed @16:24:54.34 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.55 @16:24:54.40 Hand.open @16:24:55.05 Forearm.reconfigure :loose @16:24:55.09 Forearm.seek :retract @16:24:56.03 PRV.dial :air @16:24:56.48 PSV.dial :mixloop @16:24:56.99 PS.seek 0.3ml @16:25:00.90 PSV.dial PSR @16:25:02.11 Pulling 0.4ml of PSR.air @16:25:02.12 PSR.select :air @16:25:02.14 PS.seek 0.7ml @16:25:07.00 delay 3 seconds @16:25:10.01 PSR.select :air @16:25:10.03 PS.seek 2.5ml @16:25:29.21 delay 3 seconds @16:25:32.23 PSV.dial :mixloop @16:25:33.26 PTV.dial :mixing @16:25:34.03 PBV.dial :waste2 @16:25:34.97 PS.seek 0ml @16:26:01.25 Garaging FlushPuck in Processing Clamp @16:26:01.32 Elbow.reconfigure :tight @16:26:01.36 Elbow.seek PC @16:26:01.70 Elbow.reconfigure :loose @16:26:01.78 Forearm.seek PC @16:26:02.74 Hand.close @16:26:03.37 PC.seek :open @16:26:08.07 Forearm.seek :retract @16:26:09.08 Elbow.reconfigure :tight @16:26:09.13 Elbow.seek :garage @16:26:10.34 Elbow.reconfigure :loose @16:26:10.43 Forearm.reconfigure :tight @16:26:10.47 Forearm.seek :garage @16:26:11.39 Hand.open @16:26:12.05 Forearm.reconfigure :loose @16:26:12.09 Forearm.seek :retract @16:26:13.53 Loading da puck from tube 2 into Processing Clamp @16:26:13.74 SC.reconfigure :tight @16:26:13.79 SC.seek 2 @16:26:17.40 SC.configure :limp @16:26:17.60 Elbow.reconfigure :tight @16:26:17.64 Elbow.seek 2 @16:26:19.50 Forearm.reconfigure :tight @16:26:19.54 Forearm.seek 2 + 450 counts @16:26:20.60 SE.reconfigure :pick @16:26:20.64 SE.seek :beamBreak @16:26:23.33 SE.configure :default @16:26:23.47 Elbow.seek 2 @16:26:23.64 Forearm.seek 2 + 300 counts @16:26:23.83 Forearm.reconfigure :loose @16:26:23.87 Hand.close @16:26:24.50 SE.seek :down @16:26:24.69
Forearm.seek :retract @16:26:25.65 Elbow.seek PC @16:26:26.92 SE.stop @16:26:28.23
Elbow.reconfigure :loose @16:26:28.32 Forearm.reconfigure :tight @16:26:28.36 Forearm.seek PC @16:26:29.60 PC.seek :closed @16:26:34.21 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.59 @16:26:34.22 Hand.open @16:26:34.93 Forearm.reconfigure :loose @16:26:34.97 Forearm.seek :retract @16:26:35.95 Heating Puck.from 2, :da in Processing Clamp to 28C for 2:00:00 @16:26:35.97 PH.seek 28C,5:00 @16:27:14.74 PH.hold 2:00:00,28C @16:27:14.78 delay 1:30 @16:28:44.82 PSR.select :air @16:28:44.84 PSV.dial PSR @16:28:45.94 Pulling 0.5ml of PSR.wash @16:28:45.95 PSR.select :wash @16:28:46.06 PS.seek 0.5ml @16:28:52.06 delay 5 seconds @16:28:57.07 PSR.select :air @16:28:57.19 PS.seek 2ml @16:29:13.27 PTV.dial :puck @16:29:13.99 PSV.dial PTV @16:29:14.76 PBV.dial :waste1 @16:29:16.15 PS.seek 1.5ml @16:29:22.05 delay 3 seconds @16:29:25.07 PBV.dial between PSV and waste1 @16:29:25.44 PS.seek 0ml @16:29:41.55 delay 5:00 @16:34:41.58 recoverPSRreagent08 PTV.dial :puck @16:34:41.59 PBV.dial :waste1 @16:34:41.96 delay 30 seconds @16:35:11.98 PBV.dial between PSV and waste1 @16:35:12.33 PSR.select :air @16:35:12.35 PSV.dial PSR @16:35:13.12 PS.reconfigure :fast @16:35:13.16 PS.seek 2.5ml @16:35:36.86 PSV.dial PTV @16:35:37.61 delay 5 seconds @16:35:42.63 PSV.dial PSR @16:35:43.38 delay 5 seconds @16:35:48.39 PSV.dial PTV @16:35:49.14 delay 5 seconds @16:35:54.16 PSV.dial :air @16:35:55.04 PS.seek 0ml @16:36:18.61 PTV.dial :PRVmixing,via: PRV @16:36:19.92 PSV.dial :mixloop @16:36:20.37 PRV.dial :ab1 @16:36:21.13 PS.reconfigure :slow @16:36:21.17 PS.seek 0.15ml @16:36:24.80 delay 2 seconds @16:36:26.81 CBV.dial PRV @16:36:26.84
PRV.dial CBV @16:36:27.39 PS.seek 0.35ml @16:36:32.01 delay 2 seconds @16:36:34.03 CBV.dial between PRV and PSV @16:36:34.34 PRV.dial :air @16:36:34.79 PS.seek 0.9ml @16:36:46.55 PRV.dial :waste2 @16:36:47.29 PS.seek 0.5ml @16:36:55.98 addPMLReagent08 @16:36:55.99 PSV.dial :air @16:36:56.46 PS.reconfigure :default @16:36:56.50 PS.seek 2ml @16:37:12.67 PSV.dial :PML @16:37:13.14 PTV.dial :mixing @16:37:13.89 PBV.dial :waste1 @16:37:14.29 PS.seek 1.85ml @16:37:16.61 PBV.dial between PSV and waste1 @16:37:16.97 PS.seek 0ml @16:37:36.70 delay 2 seconds @16:37:38.72 Beginning samplePassC @16:37:38.73 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @16:37:38.89 Elbow.reconfigure :tight @16:37:38.93 Elbow.seek FlushPuck::Garage @16:37:40.33 Elbow.reconfigure :loose @16:37:40.42 Forearm.seek :garage @16:37:41.40 Hand.close @16:37:42.09 Forearm.seek :retract @16:37:43.06 Elbow.reconfigure :tight @16:37:43.10 Elbow.seek CC @16:37:45.95 Elbow.reconfigure :loose @16:37:46.05 Forearm.reconfigure :tight @16:37:46.09 Forearm.seek CC @16:37:47.25 CC.seek :closed @16:37:51.78 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.38 @16:37:51.86 Hand.open @16:37:52.54 Forearm.reconfigure :loose @16:37:52.58 Forearm.seek :retract @16:37:53.60 PSV.dial PSR @16:37:54.69 Pulling 1.5ml of PSR.flush @16:37:54.70 PSR.select :flush @16:37:54.82 PS.seek 1.5ml @16:38:10.95 delay 5 seconds @16:38:15.97 PSV.dial :waste1 @16:38:16.42 PS.seek 0ml @16:38:32.51 PSV.dial PSR @16:38:32.96 PSR.select :air @16:38:33.07 PS.seek 2.5ml @16:38:59.46 PRV.dial CBV,avoiding: :ab2 @16:38:59.47
CBV.dial PRV @16:39:01.11 PSV.dial :mixloop @16:39:02.14 CTV.dial :air,avoiding: :intake @16:39:02.15 PTV.dial :PRVmixing @16:39:02.89 PS.seek 0ml @16:39:29.42 PSV.dial :air @16:39:29.88 PS.seek 2.5ml @16:39:56.23 PSV.dial :mixloop @16:39:56.69 PS.seek 0ml @16:40:23.07 PSV.dial :air @16:40:23.53 PS.seek 2.5ml @16:40:49.82 PSV.dial :mixloop @16:40:50.28 PS.seek 0ml @16:41:16.67 Garaging FlushPuck in Collection Clamp @16:41:17.18 Elbow.reconfigure :tight @16:41:17.24 Elbow.seek CC @16:41:17.67 Elbow.reconfigure :loose @16:41:17.77 Forearm.seek CC @16:41:18.71 Hand.close @16:41:19.36 CC.seek :open @16:41:23.96 Forearm.seek :retract @16:41:24.95 Elbow.reconfigure :tight @16:41:24.99 Elbow.seek :garage @16:41:27.83 Elbow.reconfigure :loose @16:41:27.97 Forearm.reconfigure :tight @16:41:28.01 Forearm.seek :garage @16:41:29.04 Hand.open @16:41:29.66 Forearm.reconfigure :loose @16:41:29.70 Forearm.seek :retract @16:41:30.67 delayUntil "16:45:38.71" @16:41:30.69 Whole Cell Archive sampling 1000ml @16:41:30.71 Loading wcr puck from tube 2 into Collection Clamp @16:41:30.94 SC.reconfigure :tight @16:41:30.99 SC.seek 2 @16:41:31.13 SC.configure :limp @16:41:31.33 Elbow.reconfigure :tight @16:41:31.37 Elbow.seek 2 @16:41:33.28 Forearm.reconfigure :tight @16:41:33.33 Forearm.seek 2 + 450 counts @16:41:34.31 SE.reconfigure :pick @16:41:34.36 SE.seek :beamBreak @16:41:37.16 SE.configure :default @16:41:37.31 Elbow.seek 2 @16:41:37.47 Forearm.seek 2 + 300 counts @16:41:37.79 Forearm.reconfigure :loose @16:41:37.83 Hand.close @16:41:38.49 SE.seek :down @16:41:38.68 Forearm.seek :retract @16:41:39.65 Elbow.seek CC @16:41:40.92 SE.stop @16:41:41.27 Elbow.reconfigure :loose @16:41:41.36 Forearm.reconfigure :tight @16:41:41.41 Forearm.seek CC @16:41:42.65 CC.seek :closed @16:41:47.16 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.22 @16:41:47.18 Hand.open @16:41:47.79 Forearm.reconfigure :loose @16:41:47.83 Forearm.seek :retract @16:41:48.87 collectSample 1000ml w/bubble point of 35psi @16:41:48.88 Priming 7ml @16:41:48.89 Intake.select :close @16:41:48.90 Exhaust.select :close @16:41:48.92 SS.hold @16:41:49.11 Calibrating pressure sensors @16:41:49.12 CTV.dial :air @16:41:49.14 SSV.dial :spit,avoiding: :exhaust @16:41:49.15 SS.reconfigure :fast @16:41:49.20 SS.seek 5ml @16:41:51.86 SSV.dial CBV @16:41:51.88 CBV.dial SSV @16:41:53.27 delay 3.8 seconds @16:41:57.19 SSV.dial :spit @16:41:58.52 SS.seek 0ml @16:42:01.31 SSV.dial CBV @16:42:02.62 CTV.dial :intake @16:42:03.85 SSV.dial CBV @16:42:03.87 CBV.dial SSV @16:42:03.90 Allowing 40s for 7.0ml of remaining 7.0ml @16:42:03.92 Intake.select :open @16:42:06.07 Intake @15.2psia, vacuum from 9.7 to 12.7psi @16:42:06.09 SS.reconfigure :inhaling 2.20ml/s @16:42:06.14 SS.seek 7ml @16:42:12.87 Stroke completed -- waiting for pressure to equalize ... @16:42:12.88 SS.reconfigure :exhale @16:42:12.93 SS.hold @16:42:21.39 Intake.select :close @16:42:21.54 SSV.dial :exhaust @16:42:22.28 Exhaust.select :open @16:42:22.39 SS.seek 0ml @16:42:26.36 Exhaust.select :close @16:42:26.46 Sampling 1000.0ml @16:42:26.47 Intake.select :close @16:42:26.48 Exhaust.select :close @16:42:26.50 SS.reconfigure :default @16:42:26.54 SS.hold @16:42:26.74 CTV.dial :intake @16:42:26.76 SSV.dial CBV @16:42:26.77 CBV.dial SSV @16:42:27.55 Allowing 130s for 25.0ml of remaining 1000.0ml @16:42:27.56 Intake.select :open @16:42:29.72 Intake @15.6psia, vacuum from 10.0 to 13.0psi @16:42:29.74 SS.reconfigure :inhaling 2.20ml/s @16:42:29.78 SS.seek 25ml @16:42:37.21 Vacuum too high -- waiting up to 40 seconds @16:42:37.22 SS.reconfigure :exhale @16:42:37.26 SS.hold @16:42:38.83 Intake @13.2psia, vacuum from 7.7 to 10.7psi @16:42:38.85 SS.reconfigure :inhaling 1.98ml/s @16:42:38.89 SS.seek 25ml @16:42:46.93 Vacuum too high -- waiting up to 40 seconds @16:42:46.94 SS.reconfigure :exhale @16:42:46.98 SS.hold @16:42:53.72 Intake @14.6psia, vacuum from 9.1 to 12.1psi @16:42:53.74 SS.reconfigure :inhaling 1.79ml/s @16:42:53.79 SS.seek 25ml @16:42:55.64 Stroke completed -- waiting for pressure to equalize ... @16:42:55.65 SS.reconfigure :exhale @16:42:55.69 SS.hold @16:42:57.25 Intake.select :close @16:42:57.40 SSV.dial :exhaust @16:42:58.15 Exhaust.select :open @16:42:58.26 SS.seek 0ml @16:43:06.95 Exhaust.select :close @16:43:07.06 SSV.dial CBV @16:43:07.08 CBV.dial SSV @16:43:08.20 Allowing 130s for 25.0ml of remaining 975.0ml @16:43:08.22 Intake.select :open @16:43:10.37 Intake @15.9psia, vacuum from 10.0 to 13.0psi @16:43:10.39 SS.reconfigure :inhaling 1.79ml/s @16:43:10.43 SS.seek 25ml @16:43:22.83 Vacuum too high -- waiting up to 40 seconds @16:43:22.84 SS.reconfigure :exhale @16:43:22.89 SS.hold @16:43:23.46 Intake @13.0psia, vacuum from 7.5 to 10.5psi @16:43:23.48 SS.reconfigure :inhaling 1.61ml/s @16:43:23.52 SS.seek 25ml @16:43:29.61 Stroke completed -- waiting for pressure to equalize ... @16:43:29.62 SS.reconfigure :exhale @16:43:29.66 SS.hold @16:43:35.62 Intake.select :close @16:43:35.76 SSV.dial :exhaust @16:43:36.51 Exhaust.select :open @16:43:36.62 SS.seek 0ml @16:43:45.43 Exhaust.select :close @16:43:45.54 SSV.dial CBV @16:43:45.56 CBV.dial SSV @16:43:46.33 Allowing 130s for 25.0ml of remaining 950.0ml @16:43:46.34 Intake.select :open @16:43:48.49 Intake @15.9psia, vacuum from 10.0 to 13.0psi @16:43:48.51 SS.reconfigure :inhaling 1.61ml/s @16:43:48.56 SS.seek 25ml @16:44:04.36 Vacuum too high -- waiting up to 40 seconds @16:44:04.37 SS.reconfigure :exhale @16:44:04.42 SS.hold @16:44:04.96 Intake @13.1psia, vacuum from 7.6 to 10.6psi @16:44:04.98 SS.reconfigure :inhaling 1.45ml/s @16:44:05.02 SS.seek 25ml @16:44:08.85 Stroke completed -- waiting for pressure to equalize ... @16:44:08.87 SS.reconfigure :exhale @16:44:08.91 SS.hold @16:44:14.63 Intake.select :close @16:44:14.77 SSV.dial :exhaust @16:44:15.51 Exhaust.select :open @16:44:15.62 SS.seek 0ml @16:44:24.34 Exhaust.select :close @16:44:24.45 SSV.dial CBV @16:44:24.48 CBV.dial SSV @16:44:25.22 Allowing 130s for 25.0ml of remaining 925.0ml @16:44:25.23 Intake.select :open @16:44:27.39 Intake @16.1psia, vacuum from 10.0 to 13.0psi @16:44:27.40 SS.reconfigure :inhaling 1.45ml/s @16:44:27.45 SS.seek 25ml @16:44:44.42 Vacuum too high -- waiting up to 40 seconds @16:44:44.44 SS.reconfigure :exhale @16:44:44.48 SS.hold @16:44:44.98 Intake @13.1psia, vacuum from 7.6 to 10.6psi @16:44:45.00 SS.reconfigure :inhaling 1.30ml/s @16:44:45.04 SS.seek 25ml @16:44:49.12 Stroke completed -- waiting for pressure to equalize ... @16:44:49.13 SS.reconfigure :exhale @16:44:49.17 SS.hold @16:44:54.83 Intake.select :close @16:44:54.97 SSV.dial :exhaust @16:44:55.72 Exhaust.select :open @16:44:55.83 SS.seek 0ml @16:45:04.56 Exhaust.select :close @16:45:04.66 SSV.dial CBV @16:45:04.68 CBV.dial SSV @16:45:05.43 Allowing 130s for 25.0ml of remaining 900.0ml @16:45:05.45 Intake.select :open @16:45:07.60 Intake @15.8psia, vacuum from 10.0 to 13.0psi @16:45:07.62 SS.reconfigure :inhaling 1.30ml/s @16:45:07.66 SS.seek 25ml @16:45:30.04 Stroke completed -- waiting for pressure to equalize ... @16:45:30.05 SS.reconfigure :exhale @16:45:30.10 SS.hold @16:45:35.64 Intake.select :close @16:45:35.78 SSV.dial :exhaust @16:45:36.54 Exhaust.select :open @16:45:36.65 SS.seek 0ml @16:45:38.72 recoverPRVreagent08 @16:45:38.73 PTV.dial :mixing @16:45:39.47 PBV.dial :waste1 @16:45:39.85 delay 30 seconds @16:45:45.37 Exhaust.select :close @16:45:45.48 SSV.dial CBV @16:45:45.50 CBV.dial SSV @16:45:46.28 Allowing 130s for 25.0ml of remaining 875.0ml @16:45:46.29 Intake.select :open @16:45:48.45 Intake @15.8psia, vacuum from 10.0 to 13.0psi @16:45:48.46 SS.reconfigure :inhaling 1.30ml/s @16:45:48.51 SS.seek 25ml @16:46:09.87 PBV.dial between PSV and waste1 @16:46:10.24 PSV.dial :air @16:46:10.52 Stroke completed -- waiting for pressure to equalize ... @16:46:10.54 SS.reconfigure :exhale @16:46:10.58 SS.hold @16:46:10.71 PS.reconfigure :fast @16:46:10.75 PS.seek 2.5ml @16:46:17.06 Intake.select :close @16:46:17.20 SSV.dial :exhaust @16:46:17.96 Exhaust.select :open @16:46:18.07 SS.seek 0ml @16:46:26.88 Exhaust.select :close @16:46:26.99 SSV.dial CBV @16:46:27.01 CBV.dial SSV @16:46:27.78 Allowing 130s for 25.0ml of remaining 850.0ml @16:46:27.79 Intake.select :open @16:46:29.94 Intake @15.8psia, vacuum from 10.0 to 13.0psi @16:46:29.96 SS.reconfigure :inhaling 1.30ml/s @16:46:30.00 SS.seek 25ml @16:46:34.47 PSV.dial :mixloop @16:46:34.94 delay 2 seconds @16:46:36.96 PSV.dial :air @16:46:37.43 delay 2 seconds @16:46:39.44 PSV.dial :mixloop @16:46:39.93 delay 2 seconds @16:46:41.95 PSV.dial :air @16:46:42.42 PS.seek 0ml @16:46:52.09 Stroke completed -- waiting for pressure to equalize ... @16:46:52.10 SS.reconfigure :exhale @16:46:52.14 SS.hold @16:46:58.56 Intake.select :close @16:46:58.70 SSV.dial :exhaust @16:46:59.46 Exhaust.select :open @16:46:59.57 SS.seek 0ml @16:47:05.60 washCycleDA #1 of 2 @16:47:05.61 PSR.select :air @16:47:05.62 PSV.dial PSR @16:47:06.99 Pulling 0.5ml of PSR.wash @16:47:07.00 PSR.select :wash @16:47:07.11 PS.reconfigure :default @16:47:07.16 PS.seek 0.5ml @16:47:08.54 Exhaust.select :close @16:47:08.65 SSV.dial CBV @16:47:08.67 CBV.dial SSV @16:47:09.43 Allowing 130s for 25.0ml of remaining 825.0ml @16:47:09.45 Intake.select :open @16:47:11.60 Intake @15.8psia, vacuum from 10.0 to 13.0psi @16:47:11.62 SS.reconfigure :inhaling 1.30ml/s @16:47:11.66 SS.seek 25ml @16:47:13.13 delay 5 seconds @16:47:18.15 PSR.select :air @16:47:18.26 PS.seek 2ml @16:47:33.35 Stroke completed -- waiting for pressure to equalize ... @16:47:33.36 SS.reconfigure :exhale @16:47:33.41 SS.hold @16:47:34.41 PTV.dial :puck @16:47:35.18 PSV.dial PTV @16:47:35.92 PBV.dial :waste1 @16:47:36.36 PS.seek 1.5ml @16:47:40.22 Intake.select :close @16:47:40.36 SSV.dial :exhaust @16:47:41.10 Exhaust.select :open @16:47:41.21 SS.seek 0ml @16:47:42.28 delay 3 seconds @16:47:45.30 PBV.dial between PSV and waste1 @16:47:45.68 PS.seek 0ml @16:47:50.02 Exhaust.select :close @16:47:50.13 SSV.dial CBV @16:47:50.15 CBV.dial SSV @16:47:50.92 Allowing 130s for 25.0ml of remaining 800.0ml @16:47:50.93 Intake.select :open @16:47:53.08 Intake @15.8psia, vacuum from 10.0 to 13.0psi @16:47:53.10 SS.reconfigure :inhaling 1.30ml/s @16:47:53.14 SS.seek 25ml @16:48:01.78 delay 2:00 @16:48:13.73 Vacuum too high -- waiting up to 40 seconds @16:48:13.75 SS.reconfigure :exhale @16:48:13.79 SS.hold @16:48:14.83 Intake @13.1psia, vacuum from 7.6 to 10.6psi @16:48:14.85 SS.reconfigure :inhaling 1.17ml/s @16:48:14.90 SS.seek 25ml @16:48:17.10 Stroke completed -- waiting for pressure to equalize ... @16:48:17.11 SS.reconfigure :exhale @16:48:17.15 SS.hold @16:48:22.79 Intake.select :close @16:48:22.93 SSV.dial :exhaust @16:48:23.68 Exhaust.select :open @16:48:23.79 SS.seek 0ml @16:48:32.63 Exhaust.select :close @16:48:32.74 SSV.dial CBV @16:48:32.76 CBV.dial SSV @16:48:33.53 Allowing 130s for 25.0ml of remaining 775.0ml @16:48:33.54 Intake.select :open @16:48:35.69 Intake @15.8psia, vacuum from 10.0 to 13.0psi @16:48:35.71 SS.reconfigure :inhaling 1.17ml/s @16:48:35.76 SS.seek 25ml @16:48:59.97 Stroke completed -- waiting for pressure to equalize ... @16:48:59.99 SS.reconfigure :exhale @16:49:00.03 SS.hold @16:49:06.44 Intake.select :close @16:49:06.58 SSV.dial :exhaust @16:49:07.32 Exhaust.select :open @16:49:07.43 SS.seek 0ml @16:49:16.46 Exhaust.select :close @16:49:16.57 SSV.dial CBV @16:49:16.61 CBV.dial SSV @16:49:17.34 Allowing 130s for 25.0ml of remaining 750.0ml @16:49:17.35 Intake.select :open @16:49:19.50 Intake @15.7psia, vacuum from 10.0 to 13.0psi @16:49:19.52 SS.reconfigure :inhaling 1.17ml/s @16:49:19.56 SS.seek 25ml @16:49:43.16 Stroke completed -- waiting for pressure to equalize ... @16:49:43.17 SS.reconfigure :exhale @16:49:43.21 SS.hold @16:49:50.86 Intake.select :close @16:49:51.00 SSV.dial :exhaust @16:49:51.77 Exhaust.select :open @16:49:51.88 SS.seek 0ml @16:50:00.64 Exhaust.select :close @16:50:00.75 SSV.dial CBV @16:50:00.77 CBV.dial SSV @16:50:01.55 Allowing 130s for 25.0ml of remaining 725.0ml @16:50:01.57 Intake.select :open @16:50:01.80 recoverPSRreagent08 @16:50:01.81 PTV.dial :puck @16:50:01.82 PBV.dial :waste1 @16:50:02.19 delay 30 seconds @16:50:03.72 Intake @15.7psia, vacuum from 10.0 to 13.0psi @16:50:03.74 SS.reconfigure :inhaling 1.17ml/s @16:50:03.78 SS.seek 25ml @16:50:28.05 Stroke completed -- waiting for pressure to equalize ... @16:50:28.06 SS.reconfigure :exhale @16:50:28.10 SS.hold @16:50:32.23 PBV.dial between PSV and waste1 @16:50:32.61 PSR.select :air @16:50:32.63 PSV.dial PSR @16:50:33.39 PS.reconfigure :fast @16:50:33.44 PS.seek 2.5ml @16:50:35.58 Intake.select :close @16:50:35.72 SSV.dial :exhaust @16:50:36.49 Exhaust.select :open @16:50:36.60 SS.seek 0ml @16:50:45.69 Exhaust.select :close @16:50:45.80 SSV.dial CBV @16:50:45.82 CBV.dial SSV @16:50:46.58 Allowing 130s for 25.0ml of remaining 700.0ml @16:50:46.60 Intake.select :open @16:50:48.75 Intake @15.7psia, vacuum from 10.0 to 13.0psi @16:50:48.77 SS.reconfigure :inhaling 1.17ml/s @16:50:48.81 SS.seek 25ml @16:50:57.09 PSV.dial PTV @16:50:57.86 delay 5 seconds @16:51:02.88 PSV.dial PSR @16:51:03.64 delay 5 seconds @16:51:08.66 PSV.dial PTV @16:51:09.42 delay 5 seconds @16:51:12.63 Stroke completed -- waiting for pressure to equalize ... @16:51:12.64 SS.reconfigure :exhale @16:51:12.68 SS.hold @16:51:14.43 PSV.dial :air @16:51:15.21 PS.seek 0ml @16:51:21.16 Intake.select :close @16:51:21.30 SSV.dial :exhaust @16:51:22.18 Exhaust.select :open @16:51:22.29 SS.seek 0ml @16:51:31.33 Exhaust.select :close @16:51:31.44 SSV.dial CBV @16:51:31.46 CBV.dial SSV @16:51:32.22 Allowing 130s for 25.0ml of remaining 675.0ml @16:51:32.24 Intake.select :open @16:51:34.39 Intake @15.6psia, vacuum from 10.0 to 13.0psi @16:51:34.41 SS.reconfigure :inhaling 1.17ml/s @16:51:34.45 SS.seek 25ml @16:51:38.43 washCycleDA #2 of 2 @16:51:38.44 PSR.select :air @16:51:38.46 PSV.dial PSR @16:51:39.83 Pulling 0.5ml of PSR.wash @16:51:39.84 PSR.select :wash @16:51:39.95 PS.reconfigure :default @16:51:39.99 PS.seek 0.5ml @16:51:45.91 delay 5 seconds @16:51:50.92 PSR.select :air @16:51:51.04 PS.seek 2ml @16:51:58.41 Stroke completed -- waiting for pressure to equalize ... @16:51:58.42 SS.reconfigure :exhale @16:51:58.46 SS.hold @16:52:06.93 Intake.select :close @16:52:07.07 SSV.dial :exhaust @16:52:07.62 PTV.dial :puck @16:52:07.63 PSV.dial PTV @16:52:07.82 Exhaust.select :open @16:52:07.93 SS.seek 0ml @16:52:08.37 PBV.dial :waste1 @16:52:08.81 PS.seek 1.5ml @16:52:14.81 delay 3 seconds @16:52:16.77 Exhaust.select :close @16:52:16.87 SSV.dial CBV @16:52:16.93 CBV.dial SSV @16:52:17.63 Allowing 130s for 25.0ml of remaining 650.0ml @16:52:17.64 Intake.select :open @16:52:17.82 PBV.dial between PSV and waste1 @16:52:18.21 PS.seek 0ml @16:52:19.80 Intake @15.7psia, vacuum from 10.0 to 13.0psi @16:52:19.82 SS.reconfigure :inhaling 1.17ml/s @16:52:19.86 SS.seek 25ml @16:52:34.34 delay 2:00 @16:52:41.26 Vacuum too high -- waiting up to 40 seconds @16:52:41.27 SS.reconfigure :exhale @16:52:41.31 SS.hold @16:52:44.33 Intake @13.5psia, vacuum from 8.0 to 11.0psi @16:52:44.35 SS.reconfigure :inhaling 1.06ml/s @16:52:44.39 SS.seek 25ml @16:52:47.84 Stroke completed -- waiting for pressure to equalize ... @16:52:47.86 SS.reconfigure :exhale @16:52:47.90 SS.hold @16:52:53.44 Intake.select :close @16:52:53.58 SSV.dial :exhaust @16:52:54.35 Exhaust.select :open @16:52:54.46 SS.seek 0ml @16:53:03.47 Exhaust.select :close @16:53:03.58 SSV.dial CBV @16:53:03.61 CBV.dial SSV @16:53:04.36 Allowing 130s for 25.0ml of remaining 625.0ml @16:53:04.38 Intake.select :open @16:53:06.53 Intake @15.8psia, vacuum from 10.0 to 13.0psi @16:53:06.55 SS.reconfigure :inhaling 1.06ml/s @16:53:06.59 SS.seek 25ml @16:53:32.48 Stroke completed -- waiting for pressure to equalize ... @16:53:32.50 SS.reconfigure :exhale @16:53:32.54 SS.hold @16:53:41.09 Intake.select :close @16:53:41.23 SSV.dial :exhaust @16:53:42.00 Exhaust.select :open @16:53:42.11 SS.seek 0ml @16:53:50.91 Exhaust.select :close @16:53:51.52 SSV.dial CBV @16:53:51.54 CBV.dial SSV @16:53:52.32 Allowing 130s for 25.0ml of remaining 600.0ml @16:53:52.33 Intake.select :open @16:53:54.48 Intake @15.6psia, vacuum from 10.0 to 13.0psi @16:53:54.50 SS.reconfigure :inhaling 1.06ml/s @16:53:54.54 SS.seek 25ml @16:54:21.03 Stroke completed -- waiting for pressure to equalize ... @16:54:21.04 SS.reconfigure :exhale @16:54:21.08 SS.hold @16:54:29.54 Intake.select :close @16:54:29.68 SSV.dial :exhaust @16:54:30.45 Exhaust.select :open @16:54:30.57 SS.seek 0ml @16:54:34.36 recoverPSRreagent08 @16:54:34.37 PTV.dial :puck @16:54:34.38 PBV.dial :waste1 @16:54:34.74 delay 30 seconds @16:54:39.31 Exhaust.select :close @16:54:39.42 SSV.dial CBV @16:54:39.45 CBV.dial SSV @16:54:40.22 Allowing 130s for 25.0ml of remaining 575.0ml @16:54:40.23 Intake.select :open @16:54:42.39 Intake @15.5psia, vacuum from 10.0 to 13.0psi @16:54:42.41 SS.reconfigure :inhaling 1.06ml/s @16:54:42.45 SS.seek 25ml @16:55:04.77 PBV.dial between PSV and waste1 @16:55:05.13 PSR.select :air @16:55:05.15 PSV.dial PSR @16:55:05.92 PS.reconfigure :fast @16:55:05.96 PS.seek 2.5ml @16:55:08.56 Stroke completed -- waiting for pressure to equalize ... @16:55:08.57 SS.reconfigure :exhale @16:55:08.62 SS.hold @16:55:18.31 Intake.select :close @16:55:18.45 SSV.dial :exhaust @16:55:19.22 Exhaust.select :open @16:55:19.33 SS.seek 0ml @16:55:28.12 Exhaust.select :close @16:55:28.23 SSV.dial CBV @16:55:28.25 CBV.dial SSV @16:55:29.03 Allowing 130s for 25.0ml of remaining 550.0ml @16:55:29.04 Intake.select :open @16:55:29.68 PSV.dial PTV @16:55:30.45 delay 5 seconds @16:55:31.20 Intake @15.5psia, vacuum from 10.0 to 13.0psi @16:55:31.21 SS.reconfigure :inhaling 1.06ml/s @16:55:31.26 SS.seek 25ml @16:55:35.46 PSV.dial PSR @16:55:36.21 delay 5 seconds @16:55:41.23 PSV.dial PTV @16:55:41.99 delay 5 seconds @16:55:47.01 PSV.dial :air @16:55:47.78 PS.seek 0ml @16:55:57.48 Stroke completed -- waiting for pressure to equalize ... @16:55:57.49 SS.reconfigure :exhale @16:55:57.54 SS.hold @16:56:07.21 Intake.select :close @16:56:07.94 SSV.dial :exhaust @16:56:08.79 Exhaust.select :open @16:56:08.91 SS.seek 0ml @16:56:10.84 getPMLReagent08 @16:56:10.85 PRV.dial :air @16:56:11.29 delay 5 seconds @16:56:16.31 PTV.dial :PRVmixing @16:56:17.63 delay 5 seconds @16:56:17.95 Exhaust.select :close @16:56:18.06 SSV.dial CBV @16:56:18.08 CBV.dial SSV @16:56:18.84 Allowing 130s for 25.0ml of remaining 525.0ml @16:56:18.86 Intake.select :open @16:56:21.01 Intake @15.5psia, vacuum from 10.0 to 13.0psi @16:56:21.03 SS.reconfigure :inhaling 1.06ml/s @16:56:21.07 SS.seek 25ml @16:56:22.65 PRV.dial :ab2 @16:56:23.12 PTV.dial :PRVmixing @16:56:23.13 PSV.dial :mixloop @16:56:23.61 PS.reconfigure :default @16:56:23.65 PS.seek 0.3ml @16:56:27.58 delay 2 seconds @16:56:29.60 PRV.dial :air @16:56:30.11 PS.seek 0.55ml @16:56:33.59 addPMLReagent08 @16:56:33.60 PSV.dial :air @16:56:34.06 PS.seek 2ml @16:56:47.15 Stroke completed -- waiting for pressure to equalize ... @16:56:47.16 SS.reconfigure :exhale @16:56:47.21 SS.hold @16:56:49.86 PSV.dial :PML @16:56:50.32 PTV.dial :mixing @16:56:51.05 PBV.dial :waste1 @16:56:51.47 PS.seek 1.85ml @16:56:53.89 PBV.dial between PSV and waste1 @16:56:54.26 PS.seek 0ml @16:56:58.23 Intake.select :close @16:56:58.37 SSV.dial :exhaust @16:56:59.12 Exhaust.select :open @16:56:59.23 SS.seek 0ml @16:57:08.09 Exhaust.select :close @16:57:08.20 SSV.dial CBV @16:57:08.23 CBV.dial SSV @16:57:08.97 Allowing 130s for 25.0ml of remaining 500.0ml @16:57:08.98 Intake.select :open @16:57:11.13 Intake @15.6psia, vacuum from 10.0 to 13.0psi @16:57:11.15 SS.reconfigure :inhaling 1.06ml/s @16:57:11.19 SS.seek 25ml @16:57:14.05 delay 2 seconds @16:57:16.07 delay 8:00 @16:57:31.79 Vacuum too high -- waiting up to 40 seconds @16:57:31.80 SS.reconfigure :exhale @16:57:31.84 SS.hold @16:57:36.01 Intake @14.1psia, vacuum from 8.6 to 11.6psi @16:57:36.03 SS.reconfigure :inhaling 0.95ml/s @16:57:36.07 SS.seek 25ml @16:57:42.98 Stroke completed -- waiting for pressure to equalize ... @16:57:42.99 SS.reconfigure :exhale @16:57:43.03 SS.hold @16:57:49.55 Intake.select :close @16:57:49.69 SSV.dial :exhaust @16:57:50.43 Exhaust.select :open @16:57:50.54 SS.seek 0ml @16:57:59.38 Exhaust.select :close @16:57:59.99 SSV.dial CBV @16:58:00.01 CBV.dial SSV @16:58:00.76 Allowing 130s for 25.0ml of remaining 475.0ml @16:58:00.78 Intake.select :open @16:58:02.93 Intake @15.6psia, vacuum from 10.0 to 13.0psi @16:58:02.95 SS.reconfigure :inhaling 0.95ml/s @16:58:02.99 SS.seek 25ml @16:58:31.88 Stroke completed -- waiting for pressure to equalize ... @16:58:31.90 SS.reconfigure :exhale @16:58:31.94 SS.hold @16:58:41.62 Intake.select :close @16:58:41.76 SSV.dial :exhaust @16:58:42.51 Exhaust.select :open @16:58:42.62 SS.seek 0ml @16:58:51.51 Exhaust.select :close @16:58:51.62 SSV.dial CBV @16:58:51.66 CBV.dial SSV @16:58:52.39 Allowing 130s for 25.0ml of remaining 450.0ml @16:58:52.40 Intake.select :open @16:58:54.55 Intake @15.6psia, vacuum from 10.0 to 13.0psi @16:58:54.57 SS.reconfigure :inhaling 0.95ml/s @16:58:54.61 SS.seek 25ml @16:59:20.09 Vacuum too high -- waiting up to 40 seconds @16:59:20.11 SS.reconfigure :exhale @16:59:20.15 SS.hold @16:59:24.32 Intake @14.1psia, vacuum from 8.6 to 11.6psi @16:59:24.34 SS.reconfigure :inhaling 0.87ml/s @16:59:24.38 SS.seek 25ml @16:59:28.62 Stroke completed -- waiting for pressure to equalize ... @16:59:28.63 SS.reconfigure :exhale @16:59:28.67 SS.hold @16:59:35.17 Intake.select :close @16:59:35.31 SSV.dial :exhaust @16:59:36.06 Exhaust.select :open @16:59:36.17 SS.seek 0ml @16:59:45.16 Exhaust.select :close @16:59:45.27 SSV.dial CBV @16:59:45.29 CBV.dial SSV @16:59:46.04 Allowing 130s for 25.0ml of remaining 425.0ml @16:59:46.05 Intake.select :open @16:59:48.21 Intake @15.7psia, vacuum from 10.0 to 13.0psi @16:59:48.23 SS.reconfigure :inhaling 0.87ml/s @16:59:48.27 SS.seek 25ml @17:00:19.13 Stroke completed -- waiting for pressure to equalize ... @17:00:19.14 SS.reconfigure :exhale @17:00:19.19 SS.hold @17:00:28.86 Intake.select :close @17:00:29.00 SSV.dial :exhaust @17:00:29.75 Exhaust.select :open @17:00:30.37 SS.seek 0ml @17:00:39.15 Exhaust.select :close @17:00:39.25 SSV.dial CBV @17:00:39.27 CBV.dial SSV @17:00:40.03 Allowing 130s for 25.0ml of remaining 400.0ml @17:00:40.04 Intake.select :open @17:00:42.19 Intake @15.7psia, vacuum from 10.0 to 13.0psi @17:00:42.21 SS.reconfigure :inhaling 0.87ml/s @17:00:42.25 SS.seek 25ml @17:01:13.68 Stroke completed -- waiting for pressure to equalize ... @17:01:13.69 SS.reconfigure :exhale @17:01:13.73 SS.hold @17:01:24.82 Intake.select :close @17:01:24.96 SSV.dial :exhaust @17:01:25.69 Exhaust.select :open @17:01:25.81 SS.seek 0ml @17:01:34.60 Exhaust.select :close @17:01:34.71 SSV.dial CBV @17:01:34.73 CBV.dial SSV @17:01:35.49 Allowing 130s for 25.0ml of remaining 375.0ml @17:01:35.51 Intake.select :open @17:01:37.66 Intake @15.5psia, vacuum from 10.0 to 13.0psi @17:01:37.68 SS.reconfigure :inhaling 0.87ml/s @17:01:37.72 SS.seek 25ml @17:02:09.24 Stroke completed -- waiting for pressure to equalize ... @17:02:09.25 SS.reconfigure :exhale @17:02:09.29 SS.hold @17:02:20.44 Intake.select :close @17:02:20.59 SSV.dial :exhaust @17:02:21.33 Exhaust.select :open @17:02:21.44 SS.seek 0ml @17:02:30.29 Exhaust.select :close @17:02:30.39 SSV.dial CBV @17:02:30.41 CBV.dial SSV @17:02:31.16 Allowing 130s for 25.0ml of remaining 350.0ml @17:02:31.18 Intake.select :open @17:02:33.33 Intake @15.5psia, vacuum from 10.0 to 13.0psi @17:02:33.35 SS.reconfigure :inhaling 0.87ml/s @17:02:33.39 SS.seek 25ml @17:03:03.96 Vacuum too high -- waiting up to 40 seconds @17:03:03.98 SS.reconfigure :exhale @17:03:04.02 SS.hold @17:03:08.89 Intake @14.1psia, vacuum from 8.6 to 11.6psi @17:03:08.91 SS.reconfigure :inhaling 0.78ml/s @17:03:08.95 SS.seek 25ml @17:03:10.21 Stroke completed -- waiting for pressure to equalize ... @17:03:10.22 SS.reconfigure :exhale @17:03:10.26 SS.hold @17:03:16.76 Intake.select :close @17:03:16.90 SSV.dial :exhaust @17:03:17.65 Exhaust.select :open @17:03:17.76 SS.seek 0ml @17:03:26.58 Exhaust.select :close @17:03:26.69 SSV.dial CBV @17:03:26.71 CBV.dial SSV @17:03:27.48 Allowing 130s for 25.0ml of remaining 325.0ml @17:03:27.49 Intake.select :open @17:03:29.64 Intake @15.6psia, vacuum from 10.0 to 13.0psi @17:03:29.66 SS.reconfigure :inhaling 0.78ml/s @17:03:29.70 SS.seek 25ml @17:04:04.44 Stroke completed -- waiting for pressure to equalize ... @17:04:04.45 SS.reconfigure :exhale @17:04:04.49 SS.hold @17:04:15.63 Intake.select :close @17:04:15.77 SSV.dial :exhaust @17:04:16.52 Exhaust.select :open @17:04:16.63 SS.seek 0ml @17:04:25.38 Exhaust.select :close @17:04:25.48 SSV.dial CBV @17:04:25.50 CBV.dial SSV @17:04:26.26 Allowing 130s for 25.0ml of remaining 300.0ml @17:04:26.27 Intake.select :open @17:04:28.42 Intake @15.6psia, vacuum from 10.0 to 13.0psi @17:04:28.44 SS.reconfigure :inhaling 0.78ml/s @17:04:28.48 SS.seek 25ml @17:05:03.25 Stroke completed -- waiting for pressure to equalize ... @17:05:03.26 SS.reconfigure :exhale @17:05:03.30 SS.hold @17:05:14.43 Intake.select :close @17:05:14.57 SSV.dial :exhaust @17:05:15.32 Exhaust.select :open @17:05:15.43 SS.seek 0ml @17:05:16.09 recoverPRVreagent08 @17:05:16.10 PTV.dial :mixing @17:05:16.11 PBV.dial :waste1 @17:05:16.48 delay 30 seconds @17:05:24.28 Exhaust.select :close @17:05:24.39 SSV.dial CBV @17:05:24.41 CBV.dial SSV @17:05:25.18 Allowing 130s for 25.0ml of remaining 275.0ml @17:05:25.19 Intake.select :open @17:05:27.34 Intake @15.8psia, vacuum from 10.0 to 13.0psi @17:05:27.36 SS.reconfigure :inhaling 0.78ml/s @17:05:27.40 SS.seek 25ml @17:05:46.50 PBV.dial between PSV and waste1 @17:05:46.87 PSV.dial :air @17:05:47.34 PS.reconfigure :fast @17:05:47.39 PS.seek 2.5ml @17:05:57.84 Vacuum too high -- waiting up to 40 seconds @17:05:57.85 SS.reconfigure :exhale @17:05:57.89 SS.hold @17:06:02.09 Intake @14.3psia, vacuum from 8.8 to 11.8psi @17:06:02.11 SS.reconfigure :inhaling 0.70ml/s @17:06:02.15 SS.seek 25ml @17:06:07.37 Stroke completed -- waiting for pressure to equalize ... @17:06:07.39 SS.reconfigure :exhale @17:06:07.93 SS.hold @17:06:11.10 PSV.dial :mixloop @17:06:11.56 delay 2 seconds @17:06:13.58 PSV.dial :air @17:06:14.04 delay 2 seconds @17:06:15.28 Intake.select :close @17:06:15.42 SSV.dial :exhaust @17:06:16.06 PSV.dial :mixloop @17:06:16.17 Exhaust.select :open @17:06:16.28 SS.seek 0ml @17:06:16.53 delay 2 seconds @17:06:18.54 PSV.dial :air @17:06:19.02 PS.seek 0ml @17:06:25.36 Exhaust.select :close @17:06:25.47 SSV.dial CBV @17:06:25.50 CBV.dial SSV @17:06:26.24 Allowing 130s for 25.0ml of remaining 250.0ml @17:06:26.25 Intake.select :open @17:06:28.40 Intake @15.7psia, vacuum from 10.0 to 13.0psi @17:06:28.42 SS.reconfigure :inhaling 0.70ml/s @17:06:28.47 SS.seek 25ml @17:06:42.32 washCycleDA #1 of 3 @17:06:42.33 PSR.select :air @17:06:42.35 PSV.dial PSR @17:06:43.72 Pulling 0.5ml of PSR.wash @17:06:43.73 PSR.select :wash @17:06:43.84 PS.reconfigure :default @17:06:43.88 PS.seek 0.5ml @17:06:49.86 delay 5 seconds @17:06:54.87 PSR.select :air @17:06:54.99 PS.seek 2ml @17:07:05.83 Stroke completed -- waiting for pressure to equalize ... @17:07:05.84 SS.reconfigure :exhale @17:07:05.88 SS.hold @17:07:11.48 PTV.dial :puck @17:07:12.23 PSV.dial PTV @17:07:12.98 PBV.dial :waste1 @17:07:13.42 PS.seek 1.5ml @17:07:17.04 Intake.select :close @17:07:17.18 SSV.dial :exhaust @17:07:17.92 Exhaust.select :open @17:07:18.03 SS.seek 0ml @17:07:19.29 delay 3 seconds @17:07:22.31 PBV.dial between PSV and waste1 @17:07:22.69 PS.seek 0ml @17:07:26.73 Exhaust.select :close @17:07:26.84 SSV.dial CBV @17:07:26.86 CBV.dial SSV @17:07:27.63 Allowing 130s for 25.0ml of remaining 225.0ml @17:07:27.64 Intake.select :open @17:07:29.79 Intake @15.7psia, vacuum from 10.0 to 13.0psi @17:07:29.81 SS.reconfigure :inhaling 0.70ml/s @17:07:29.85 SS.seek 25ml @17:07:38.79 delay 2:00 @17:08:08.00 Stroke completed -- waiting for pressure to equalize ... @17:08:08.01 SS.reconfigure :exhale @17:08:08.05 SS.hold @17:08:19.58 Intake.select :close @17:08:19.72 SSV.dial :exhaust @17:08:20.48 Exhaust.select :open @17:08:20.59 SS.seek 0ml @17:08:29.72 Exhaust.select :close @17:08:29.83 SSV.dial CBV @17:08:29.86 CBV.dial SSV @17:08:30.61 Allowing 130s for 25.0ml of remaining 200.0ml @17:08:30.62 Intake.select :open @17:08:32.78 Intake @15.5psia, vacuum from 10.0 to 13.0psi @17:08:32.80 SS.reconfigure :inhaling 0.70ml/s @17:08:32.84 SS.seek 25ml @17:09:10.12 Stroke completed -- waiting for pressure to equalize ... @17:09:10.13 SS.reconfigure :exhale @17:09:10.18 SS.hold @17:09:22.74 Intake.select :close @17:09:22.88 SSV.dial :exhaust @17:09:23.64 Exhaust.select :open @17:09:23.75 SS.seek 0ml @17:09:32.51 Exhaust.select :close @17:09:32.62 SSV.dial CBV @17:09:32.64 CBV.dial SSV @17:09:33.42 Allowing 130s for 25.0ml of remaining 175.0ml @17:09:33.43 Intake.select :open @17:09:35.59 Intake @15.6psia, vacuum from 10.0 to 13.0psi @17:09:35.61 SS.reconfigure :inhaling 0.70ml/s @17:09:35.66 SS.seek 25ml @17:09:38.81 recoverPSRreagent08 @17:09:38.82 PTV.dial :puck @17:09:38.83 PBV.dial :waste1 @17:09:39.20 delay 30 seconds @17:10:09.22 PBV.dial between PSV and waste1 @17:10:09.59 PSR.select :air @17:10:09.60 PSV.dial PSR @17:10:10.37 PS.reconfigure :fast @17:10:10.42 PS.seek 2.5ml @17:10:13.79 Stroke completed -- waiting for pressure to equalize ... @17:10:13.80 SS.reconfigure :exhale @17:10:13.85 SS.hold @17:10:26.45 Intake.select :close @17:10:26.59 SSV.dial :exhaust @17:10:27.36 Exhaust.select :open @17:10:27.47 SS.seek 0ml @17:10:34.38 PSV.dial PTV @17:10:35.15 delay 5 seconds @17:10:36.56 Exhaust.select :close @17:10:36.66 SSV.dial CBV @17:10:36.69 CBV.dial SSV @17:10:37.43 Allowing 130s for 25.0ml of remaining 150.0ml @17:10:37.45 Intake.select :open @17:10:39.60 Intake @15.5psia, vacuum from 10.0 to 13.0psi @17:10:39.62 SS.reconfigure :inhaling 0.70ml/s @17:10:39.66 SS.seek 25ml @17:10:40.17 PSV.dial PSR @17:10:40.91 delay 5 seconds @17:10:45.93 PSV.dial PTV @17:10:46.69 delay 5 seconds @17:10:51.71 PSV.dial :air @17:10:52.47 PS.seek 0ml @17:11:15.76 washCycleDA #2 of 3 @17:11:15.77 PSR.select :air @17:11:15.78 PSV.dial PSR @17:11:17.15 Pulling 0.5ml of PSR.wash @17:11:17.16 PSR.select :wash @17:11:17.23 Stroke completed -- waiting for pressure to equalize ... @17:11:17.24 SS.reconfigure :exhale @17:11:17.28 SS.hold @17:11:17.36 PS.reconfigure :default @17:11:17.40 PS.seek 0.5ml @17:11:23.41 delay 5 seconds @17:11:28.43 PSR.select :air @17:11:28.54 PS.seek 2ml @17:11:30.37 Intake.select :close @17:11:30.52 SSV.dial :exhaust @17:11:31.26 Exhaust.select :open @17:11:31.37 SS.seek 0ml @17:11:40.20 Exhaust.select :close @17:11:40.31 SSV.dial CBV @17:11:40.34 CBV.dial SSV @17:11:41.07 Allowing 130s for 25.0ml of remaining 125.0ml @17:11:41.09 Intake.select :open @17:11:43.24 Intake @15.6psia, vacuum from 10.0 to 13.0psi @17:11:43.26 SS.reconfigure :inhaling 0.70ml/s @17:11:43.30 SS.seek 25ml @17:11:44.69 PTV.dial :puck @17:11:44.70 PSV.dial PTV @17:11:45.44 PBV.dial :waste1 @17:11:45.88 PS.seek 1.5ml @17:11:51.88 delay 3 seconds @17:11:54.90 PBV.dial between PSV and waste1 @17:11:55.28 PS.seek 0ml @17:12:11.41 delay 2:00 @17:12:12.80 Vacuum too high -- waiting up to 40 seconds @17:12:12.81 SS.reconfigure :exhale @17:12:12.85 SS.hold @17:12:18.52 Intake @14.5psia, vacuum from 9.0 to 12.0psi @17:12:18.54 SS.reconfigure :inhaling 0.63ml/s @17:12:18.58 SS.seek 25ml @17:12:28.34 Stroke completed -- waiting for pressure to equalize ... @17:12:28.35 SS.reconfigure :exhale @17:12:28.39 SS.hold @17:12:38.06 Intake.select :close @17:12:38.20 SSV.dial :exhaust @17:12:38.95 Exhaust.select :open @17:12:39.06 SS.seek 0ml @17:12:47.91 Exhaust.select :close @17:12:48.02 SSV.dial CBV @17:12:48.04 CBV.dial SSV @17:12:48.79 Allowing 130s for 25.0ml of remaining 100.0ml @17:12:48.80 Intake.select :open @17:12:50.96 Intake @15.6psia, vacuum from 10.0 to 13.0psi @17:12:50.98 SS.reconfigure :inhaling 0.63ml/s @17:12:51.02 SS.seek 25ml @17:13:33.08 Stroke completed -- waiting for pressure to equalize ... @17:13:33.10 SS.reconfigure :exhale @17:13:33.14 SS.hold @17:13:47.49 Intake.select :close @17:13:47.64 SSV.dial :exhaust @17:13:48.38 Exhaust.select :open @17:13:48.49 SS.seek 0ml @17:13:57.29 Exhaust.select :close @17:13:57.39 SSV.dial CBV @17:13:57.41 CBV.dial SSV @17:13:58.17 Allowing 130s for 25.0ml of remaining 75.0ml @17:13:58.18 Intake.select :open @17:14:00.33 Intake @15.7psia, vacuum from 10.0 to 13.0psi @17:14:00.35 SS.reconfigure :inhaling 0.63ml/s @17:14:00.39 SS.seek 25ml @17:14:11.44 recoverPSRreagent08 @17:14:11.45 PTV.dial :puck @17:14:11.46 PBV.dial :waste1 @17:14:11.83 delay 30 seconds @17:14:38.14 Vacuum too high -- waiting up to 40 seconds @17:14:38.15 SS.reconfigure :exhale @17:14:38.19 SS.hold @17:14:41.85 PBV.dial between PSV and waste1 @17:14:42.22 PSR.select :air @17:14:42.24 PSV.dial PSR @17:14:43.04 PS.reconfigure :fast @17:14:43.11 PS.seek 2.5ml @17:14:43.99 Intake @14.7psia, vacuum from 9.2 to 12.2psi @17:14:44.01 SS.reconfigure :inhaling 0.57ml/s @17:14:44.05 SS.seek 25ml @17:14:49.30 Stroke completed -- waiting for pressure to equalize ... @17:14:49.31 SS.reconfigure :exhale @17:14:49.36 SS.hold @17:14:57.82 Intake.select :close @17:14:57.96 SSV.dial :exhaust @17:14:58.71 Exhaust.select :open @17:14:58.82 SS.seek 0ml @17:15:06.90 PSV.dial PTV @17:15:07.58 Exhaust.select :close @17:15:07.67 delay 5 seconds @17:15:07.70 SSV.dial CBV @17:15:07.72 CBV.dial SSV @17:15:08.48 Allowing 130s for 25.0ml of remaining 50.0ml @17:15:08.49 Intake.select :open @17:15:10.64 Intake @15.8psia, vacuum from 10.0 to 13.0psi @17:15:10.66 SS.reconfigure :inhaling 0.57ml/s @17:15:10.70 SS.seek 25ml @17:15:12.69 PSV.dial PSR @17:15:13.46 delay 5 seconds @17:15:18.47 PSV.dial PTV @17:15:19.24 delay 5 seconds @17:15:24.25 PSV.dial :air @17:15:25.02 PS.seek 0ml @17:15:48.13 washCycleDA #3 of 3 @17:15:48.14 PSR.select :air @17:15:48.15 PSV.dial PSR @17:15:49.52 Pulling 0.5ml of PSR.wash @17:15:49.53 PSR.select :wash @17:15:49.64 PS.reconfigure :default @17:15:49.68 PS.seek 0.5ml @17:15:55.66 delay 5 seconds @17:15:56.83 Stroke completed -- waiting for pressure to equalize ... @17:15:56.84 SS.reconfigure :exhale @17:15:56.89 SS.hold @17:16:00.67 PSR.select :air @17:16:00.79 PS.seek 2ml @17:16:11.25 Intake.select :close @17:16:11.40 SSV.dial :exhaust @17:16:12.15 Exhaust.select :open @17:16:12.26 SS.seek 0ml @17:16:16.87 PTV.dial :puck @17:16:16.89 PSV.dial PTV @17:16:17.62 PBV.dial :waste1 @17:16:18.06 PS.seek 1.5ml @17:16:21.10 Exhaust.select :close @17:16:21.20 SSV.dial CBV @17:16:21.23 CBV.dial SSV @17:16:21.97 Allowing 130s for 25.0ml of remaining 25.0ml @17:16:21.99 Intake.select :open @17:16:24.03 delay 3 seconds @17:16:24.14 Intake @15.7psia, vacuum from 10.0 to 13.0psi @17:16:24.16 SS.reconfigure :inhaling 0.57ml/s @17:16:24.20 SS.seek 24.97ml @17:16:27.05 PBV.dial between PSV and waste1 @17:16:27.41 PS.seek 0ml @17:16:43.53 delay 2:00 @17:17:10.39 Stroke completed -- waiting for pressure to equalize ... @17:17:10.41 SS.reconfigure :exhale @17:17:10.45 SS.hold @17:17:24.73 Intake.select :close @17:17:24.87 SSV.dial :exhaust @17:17:25.61 Exhaust.select :open @17:17:25.72 SS.seek 0ml @17:17:34.58 Exhaust.select :close @17:17:34.69 Sampled 1000.0ml @17:17:34.70 Drying puck before fixing for 20:00 @17:17:34.71 CBV.dial :waste2b @17:17:35.46 delay 1:00 @17:18:35.47 CSV.dial :air @17:18:35.93 CS.reconfigure :fast @17:18:35.97 CS.seek 10ml @17:18:43.56 recoverPSRreagent08 @17:18:43.57 PTV.dial :puck @17:18:43.58 PBV.dial :waste1 @17:18:43.93 delay 30 seconds @17:19:00.35 Pumping wcr puck from tube 2 up to 35psi... @17:19:00.36 CBV.dial SSV @17:19:01.68 CBV.dial between CSV and waste2b @17:19:02.64 CSV.dial between air and CTV @17:19:02.96 CS.seek 7.2ml @17:19:10.07 CTV.dial CSV,avoiding: :air @17:19:10.11 CSV.dial CTV @17:19:10.89 CS.reconfigure :pressurize @17:19:10.93 CS.seek 0ml @17:19:13.95 PBV.dial between PSV and waste1 @17:19:14.31 PSR.select :air @17:19:14.33 PSV.dial PSR @17:19:15.09 PS.reconfigure :fast @17:19:15.14 PS.seek 2.5ml @17:19:24.68 CBV.dial :waste2b @17:19:25.00 CS.reconfigure :default @17:19:25.05 CS.seek 0ml @17:19:30.94 delay 1:00 @17:19:38.90 PSV.dial PTV @17:19:39.67 delay 5 seconds @17:19:44.69 PSV.dial PSR @17:19:45.43 delay 5 seconds @17:19:50.45 PSV.dial PTV @17:19:51.20 delay 5 seconds @17:19:56.22 PSV.dial :air @17:19:56.98 PS.seek 0ml @17:20:20.62 Gate.power :camera,:ON @17:20:20.63 Mixing DA Substrates @17:20:20.64 PSR.select :air @17:20:20.65 PSV.dial PSR @17:20:22.19 Pulling 0.25ml of PSR.sub1 @17:20:22.22 PSR.select :sub1 @17:20:22.39 PS.reconfigure :default @17:20:22.49 PS.seek 0.25ml @17:20:26.00 delay 5 seconds @17:20:30.96 CTV.dial :air,avoiding: :intake @17:20:31.06 Pulling 0.25ml of PSR.sub2 @17:20:31.07 PSR.select :sub2 @17:20:31.19 PS.seek 0.5ml @17:20:32.33 Fix #1 of 2 (with RNAlater) for 10:00 @17:20:32.35 CSV.dial CSR @17:20:32.84 Pulling 2ml of CSR.RNAlater @17:20:32.85 CSR.select :RNAlater @17:20:32.96 CS.seek 2ml @17:20:34.56 delay 5 seconds @17:20:38.87 delay 5 seconds @17:20:39.57 PSR.select :air @17:20:39.68 PS.seek 2ml @17:20:43.89 CSR.select :air @17:20:44.01 CS.reconfigure :fast @17:20:44.05 CS.seek 10ml @17:20:55.84 PTV.dial :puck @17:20:55.85 PSV.dial PTV @17:20:56.59 PBV.dial :waste1 @17:20:57.02 PS.seek 1.5ml @17:21:02.99 delay 3 seconds @17:21:03.81 Pumping wcr puck from tube 2 up to 35psi... @17:21:03.83 CBV.dial SSV @17:21:05.14 CBV.dial between CSV and waste2b @17:21:06.01 PBV.dial between PSV and waste1 @17:21:06.12 CSV.dial between air and CTV @17:21:06.39 PS.seek 0ml @17:21:06.73 CS.seek 7.234ml @17:21:13.88 CTV.dial CSV,avoiding: :air @17:21:13.90 CSV.dial CTV @17:21:15.28 CS.reconfigure :pressurize @17:21:15.33 CS.seek 0ml @17:21:22.52 delay 10 seconds @17:21:29.58 delay 10:00 @17:21:32.55 recoverPSRreagent08 @17:21:32.56 PTV.dial :puck @17:21:32.57 PBV.dial :waste1 @17:21:32.93 delay 30 seconds @17:22:02.96 PBV.dial between PSV and waste1 @17:22:03.32 PSR.select :air @17:22:03.34 PSV.dial PSR @17:22:04.23 PS.reconfigure :fast @17:22:04.28 PS.seek 2.5ml @17:22:28.18 PSV.dial PTV @17:22:28.93 delay 5 seconds @17:22:33.95 PSV.dial PSR @17:22:34.70 delay 5 seconds @17:22:39.71 PSV.dial PTV @17:22:40.48 delay 5 seconds @17:22:45.50 PSV.dial :air @17:22:46.41 PS.seek 0ml @17:23:09.90 Additional air clear @17:23:09.91 PSR.select :air @17:23:09.92 PSV.dial PSR @17:23:11.42 Pulling 2ml of PSR.air @17:23:11.43 PSR.select :air @17:23:11.45 PS.reconfigure :default @17:23:11.49 PS.seek 2ml @17:23:32.68 delay 5 seconds @17:23:37.70 PTV.dial :puck @17:23:37.72 PSV.dial PTV @17:23:38.46 PBV.dial :waste1 @17:23:38.89 PS.seek 1.5ml @17:23:44.83 delay 3 seconds @17:23:47.85 PBV.dial between PSV and waste1 @17:23:48.23 delay 1 second @17:23:49.24 PSV.dial :waste1 @17:23:50.31 PS.seek 0ml @17:24:06.57 Processing Puck Heater at 28.1C before imaging @17:24:06.58 PH.coast @17:24:06.71 Imaging da puck in Processing Clamp while storing it to tube 1 @17:24:06.87 SC.reconfigure :tight @17:24:06.91 SC.seek 1 @17:24:10.61 SC.configure :limp @17:24:10.80 Elbow.reconfigure :tight @17:24:10.85 Elbow.seek 1 @17:24:12.54 Forearm.reconfigure :tight @17:24:12.58 Forearm.seek 1 + 450 counts @17:24:13.60 SE.reconfigure :pick @17:24:13.65 SE.seek :beamBreak @17:24:15.11 SE.configure :default @17:24:15.25 Elbow.reconfigure :limp @17:24:15.80 Forearm.reconfigure :loose @17:24:15.84 Forearm.seek 1 @17:24:16.10 Elbow.hold @17:24:16.16 Elbow.reconfigure :tight @17:24:16.25 SE.seek 25.08 Pucks below Top Plate @17:24:16.81 Forearm.force -20 @17:24:16.85 Forearm.seek 1 + 315 counts @17:24:17.12 Forearm.reconfigure :tight @17:24:17.16 Forearm.seek 1 + 450 counts @17:24:17.37 SE.seek 26.08 Pucks below Top Plate @17:24:17.93 Forearm.reconfigure :loose @17:24:17.97 Forearm.seek :retract @17:24:18.90 Elbow.seek PC @17:24:21.42 Elbow.reconfigure :loose @17:24:21.51 Forearm.seek PC @17:24:22.42 Hand.close @17:24:23.07 PC.seek :open @17:24:27.87 Forearm.seek :retract @17:24:28.86 Elbow.reconfigure :tight @17:24:28.91 Elbow.seek 1 @17:24:31.63 Forearm.reconfigure :tight @17:24:31.67 Forearm.seek 1 + 450 counts @17:24:32.83 Hand.open @17:24:33.50 SE.seek 25.73 Pucks below Top Plate @17:24:33.75 SE.reconfigure :seal @17:24:33.80 SE.seek 24.76 Pucks below Top Plate @17:24:34.33 Lights, camera, ACTION! @17:24:34.36 PuckCamera: snap -bin=2,2 -auto /var/log/esp/da22dec1317h1000ml.tif @17:24:53.85 Waiting >20 seconds for da to relinquish FlushPuck @17:24:54.75 SX HX9/16 (USB:07): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 17.056 seconds /var/log/esp/da22dec1317h1000ml.tif: TIFF Upload Complete @17:24:54.77 PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da22dec1317h1000ml15s.tif @17:25:10.39 SX HX9/16 (USB:07): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15 seconds /var/log/esp/midres/da22dec1317h1000ml15s.tif: TIFF Upload Complete @17:25:10.41 PuckCamera: snap -bin=2,2 20.0 /var/log/esp/midres/da22dec1317h1000ml20s.tif @17:25:31.07 SX HX9/16 (USB:07): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 20 seconds /var/log/esp/midres/da22dec1317h1000ml20s.tif: TIFF Upload Complete @17:25:31.08 Gate.power :camera,:OFF @17:25:31.10 DAfans.email "17.056 sec auto-exposure",Subject: "da22dec1317h1000ml images!" @17:25:31.35 Elbow.seek 1 @17:25:31.89 Forearm.seek 1 + 450 counts @17:25:32.04 SE.reconfigure :gently @17:25:32.09 SE.seek 25.48 Pucks below Top Plate @17:25:32.79 Elbow.reconfigure :limp @17:25:32.84 Forearm.reconfigure :loose @17:25:32.88 Forearm.seek 1 @17:25:33.17 Elbow.hold @17:25:33.24 Elbow.reconfigure :tight @17:25:33.31 SE.reconfigure :default @17:25:33.35 SE.seek 25.66 Pucks below Top Plate @17:25:33.80 Forearm.force -20 @17:25:33.84 Forearm.seek 1 + 291 counts @17:25:34.17 Forearm.reconfigure :tight @17:25:34.22 Forearm.seek 1 + 450 counts @17:25:34.48 SE.seek :down @17:25:35.44 SE.stop @17:25:35.61 Forearm.reconfigure :loose @17:25:35.65 Forearm.seek :retract @17:25:36.74 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @17:25:37.10 Elbow.seek FlushPuck::Garage @17:25:39.00 Elbow.reconfigure :loose @17:25:39.28 Forearm.seek :garage @17:25:40.29 Hand.close @17:25:42.32 Forearm.seek :retract @17:25:43.35 Elbow.reconfigure :tight @17:25:43.43 Elbow.seek PC @17:25:44.97 Elbow.reconfigure :loose @17:25:45.15 Forearm.reconfigure :tight @17:25:45.23 Forearm.seek PC @17:25:46.75 PC.seek :closed @17:25:51.52 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.58 @17:25:51.70 Hand.open @17:25:52.46 Forearm.reconfigure :loose @17:25:52.54 Forearm.seek :retract @17:25:53.60 PSR.select :air @17:25:53.64 PSV.dial PSR @17:25:54.26 Pulling 0.5ml of PSR.flush @17:25:54.28 PSR.select :flush @17:25:54.46 PS.seek 0.5ml @17:26:00.48 delay 5 seconds @17:26:05.52 PSR.select :air @17:26:05.67 PS.seek 2.5ml @17:26:06.50 upsync @17:26:26.86 delay 1 second @17:26:27.88 PRV.dial :waste2,avoiding: :ab2 @17:26:29.75 PTV.dial :PRVmixing @17:26:31.06 PSV.dial :mixloop @17:26:32.10 PS.seek :empty @17:26:58.55 PSR.select :air @17:26:58.56 PSV.dial PSR @17:26:59.66 Pulling 0.5ml of PSR.flush @17:26:59.67 PSR.select :flush @17:26:59.79 PS.seek 0.5ml @17:27:05.77 delay 5 seconds @17:27:10.79 PSR.select :air @17:27:10.91 PS.seek 2.5ml @17:27:32.08 delay 1 second @17:27:33.10 PBV.dial :waste1 @17:27:33.47 PTV.dial :mixing @17:27:34.22 PSV.dial :mixloop @17:27:35.27 PS.seek :empty @17:28:01.55 PSV.dial PSR @17:28:02.59 PSR.select :flush @17:28:02.71 PS.seek 1ml @17:28:13.83 PSR.select :air @17:28:13.94 PS.seek 2.5ml @17:28:30.14 PSV.dial PTV @17:28:30.89 PTV.dial :puck @17:28:31.62 PBV.dial :waste1 @17:28:31.64 PS.seek 0ml @17:28:57.98 PSV.dial PSR @17:28:58.73 PSR.select :flush @17:28:58.85 PS.seek 0.5ml @17:29:04.95 PSR.select :air @17:29:05.06 PS.seek 2.5ml @17:29:26.39 PSV.dial PTV @17:29:27.14 PBV.dial PSV @17:29:27.71 PS.seek 0ml @17:29:54.01 PSV.dial PBV @17:29:54.49 PS.seek 2.5ml @17:30:20.79 PSV.dial :waste1 @17:30:21.55 PS.seek 0ml @17:30:47.95 PSV.dial PSR @17:30:48.42 PSR.select :air @17:30:48.44 PS.seek 2.5ml @17:31:14.82 PSV.dial PTV @17:31:15.68 PBV.dial PSV @17:31:15.70 PS.seek 0ml @17:31:29.60 CBV.dial :waste2b @17:31:29.93 CS.reconfigure :default @17:31:29.98 CS.seek 0ml @17:31:35.40 delay 1:00 @17:31:42.07 PSV.dial PBV @17:31:42.53 PS.seek 2.5ml @17:32:08.92 PSV.dial :waste1 @17:32:09.78 PS.seek 0ml @17:32:35.42 CTV.dial :air,avoiding: :intake @17:32:36.32 PSV.dial PSR @17:32:36.74 Fix #2 of 2 (with RNAlater) for 10:00 @17:32:36.75 CSV.dial CSR @17:32:36.82 PSR.select :flush @17:32:37.43 PS.seek 1ml @17:32:37.54 Pulling 2ml of CSR.RNAlater @17:32:37.55 CSR.select :RNAlater @17:32:37.66 CS.seek 2ml @17:32:43.58 delay 5 seconds @17:32:48.60 CSR.select :air @17:32:48.73 CS.reconfigure :fast @17:32:48.75 PSR.select :air @17:32:48.85 CS.seek 10ml @17:32:48.92 PS.seek 2.5ml @17:33:05.10 PSV.dial PTV @17:33:05.84 PTV.dial :puck @17:33:05.85 PBV.dial :waste1 @17:33:06.41 PS.seek 0ml @17:33:08.30 Pumping wcr puck from tube 2 up to 35psi... @17:33:08.31 CBV.dial SSV @17:33:09.63 CBV.dial between CSV and waste2b @17:33:10.59 CSV.dial between air and CTV @17:33:11.20 CS.seek 7.171ml @17:33:18.46 CTV.dial CSV,avoiding: :air @17:33:18.48 CSV.dial CTV @17:33:19.86 CS.reconfigure :pressurize @17:33:19.90 CS.seek 0ml @17:33:32.76 PSV.dial PSR @17:33:33.51 PSR.select :flush @17:33:33.63 PS.seek 0.5ml @17:33:33.76 delay 10:00 @17:33:39.63 PSR.select :air @17:33:39.74 PS.seek 2.5ml @17:34:01.01 PSV.dial PTV @17:34:01.76 PBV.dial PSV @17:34:02.33 PS.seek 0ml @17:34:28.63 PSV.dial PBV @17:34:29.11 PS.seek 2.5ml @17:34:55.44 PSV.dial :waste1 @17:34:56.19 PS.seek 0ml @17:35:22.54 PSV.dial PSR @17:35:23.01 PSR.select :air @17:35:23.03 PS.seek 2.5ml @17:35:49.38 PSV.dial PTV @17:35:50.12 PBV.dial PSV @17:35:50.14 PS.seek 0ml @17:36:16.47 PSV.dial PBV @17:36:16.95 PS.seek 2.5ml @17:36:43.25 PSV.dial :waste1 @17:36:43.98 PS.seek 0ml @17:37:10.29 Garaging FlushPuck in Processing Clamp @17:37:10.36 Elbow.reconfigure :tight @17:37:10.40 Elbow.seek PC @17:37:10.51 Elbow.reconfigure :loose @17:37:10.60 Forearm.seek PC @17:37:11.54 Hand.close @17:37:12.18 PC.seek :open @17:37:16.92 Forearm.seek :retract @17:37:17.87 Elbow.reconfigure :tight @17:37:17.91 Elbow.seek :garage @17:37:19.30 Elbow.reconfigure :loose @17:37:19.38 Forearm.reconfigure :tight @17:37:19.43 Forearm.seek :garage @17:37:20.46 Hand.open @17:37:21.11 Forearm.reconfigure :loose @17:37:21.16 Forearm.seek :retract @17:43:33.78 CBV.dial :waste2b @17:43:34.10 CS.reconfigure :default @17:43:34.15 CS.seek 0ml @17:43:39.91 delay 1:00 @17:44:39.94 CTV.dial :air,avoiding: :intake @17:44:41.30 Storing wcr puck in Collection Clamp to tube 1 @17:44:41.67 Elbow.reconfigure :tight @17:44:41.72 Elbow.seek 1 @17:44:43.61 Forearm.reconfigure :tight @17:44:44.11 Forearm.seek 1 + 450 counts @17:44:45.15 SE.reconfigure :pick @17:44:45.20 SE.seek :beamBreak @17:44:46.61 SE.configure :default @17:44:46.76 Elbow.reconfigure :limp @17:44:46.81 Forearm.reconfigure :loose @17:44:46.85 Forearm.seek 1 @17:44:47.09 Elbow.hold @17:44:47.16 Elbow.reconfigure :tight @17:44:47.25 SE.seek 26.07 Pucks below Top Plate @17:44:47.76 Forearm.force -20 @17:44:47.80 Forearm.seek 1 + 305 counts @17:44:48.10 Forearm.reconfigure :tight @17:44:48.15 Forearm.seek 1 + 450 counts @17:44:48.35 SE.seek 27.07 Pucks below Top Plate @17:44:48.96 Forearm.reconfigure :loose @17:44:49.00 Forearm.seek :retract @17:44:49.94 Elbow.seek CC @17:44:51.57 Elbow.reconfigure :loose @17:44:51.67 Forearm.seek CC @17:44:52.67 Hand.close @17:44:53.30 CC.seek :open @17:44:58.03 Forearm.seek :retract @17:44:59.07 Elbow.reconfigure :tight @17:44:59.11 Elbow.seek 1 @17:45:00.91 Elbow.reconfigure :limp @17:45:00.96 Forearm.seek 1 @17:45:01.95 Elbow.hold @17:45:02.02 Elbow.reconfigure :tight @17:45:02.06 Hand.open @17:45:02.79 Forearm.force -20 @17:45:02.83 Forearm.seek 1 + 313 counts @17:45:03.13 Forearm.reconfigure :tight @17:45:03.17 Forearm.seek 1 + 450 counts @17:45:03.43 SE.seek :down @17:45:04.34 SE.stop @17:45:04.55 Forearm.reconfigure :loose @17:45:04.60 Forearm.seek :retract @17:45:05.55 Starting finalPurge @17:45:05.56 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @17:45:05.74 Elbow.seek FlushPuck::Garage @17:45:07.55 Elbow.reconfigure :loose @17:45:07.64 Forearm.seek :garage @17:45:08.62 Hand.close @17:45:09.28 Forearm.seek :retract @17:45:10.21 Elbow.reconfigure :tight @17:45:10.25 Elbow.seek CC @17:45:13.18 Elbow.reconfigure :loose @17:45:13.27 Forearm.reconfigure :tight @17:45:13.31 Forearm.seek CC @17:45:14.48 CC.seek :closed @17:45:18.99 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.23 @17:45:19.06 Hand.open @17:45:19.73 Forearm.reconfigure :loose @17:45:20.25 Forearm.seek :retract @17:45:21.19 Flushing the Collection Valve::Manifold @17:45:21.31 CSV.dial CSR @17:45:21.79 Pulling 5ml of CSR.flush @17:45:21.80 CSR.select :flush @17:45:21.91 CS.seek 5ml @17:45:35.66 delay 5 seconds @17:45:40.68 CSR.select :air @17:45:40.82 CS.reconfigure :fast @17:45:40.87 CS.seek 7.501ml @17:45:47.59 CSV.dial :waste @17:45:48.87 CS.reconfigure :default @17:45:48.91 CS.seek 0ml @17:46:08.88 Flushing lower manifold's waste1 port @17:46:09.00 CSV.dial :flush @17:46:09.43 CS.reconfigure :fast @17:46:09.47 CS.seek 5ml @17:46:22.05 delay 5 seconds @17:46:27.07 CSV.dial :air @17:46:27.56 CS.seek 7.001ml @17:46:33.03 CSV.dial CTV @17:46:33.06
CTV.dial CSV @17:46:34.42 CBV.dial :waste1 @17:46:35.18 CS.seek 0ml @17:46:51.85 Flushing lower manifold's waste2 port @17:46:51.96 CSV.dial :flush @17:46:52.81 CS.seek 5ml @17:47:05.59 delay 5 seconds @17:47:10.61 CSV.dial :air @17:47:11.11 CS.seek 7.001ml @17:47:16.53 CSV.dial CTV @17:47:16.55
CTV.dial CSV @17:47:17.00 CBV.dial :waste2 @17:47:18.05 CS.seek 0ml @17:47:34.53 Flushing lower manifold's waste2b port @17:47:34.65 CSV.dial :flush @17:47:35.38 CS.seek 5ml @17:47:47.91 delay 5 seconds @17:47:52.92 CSV.dial :air @17:47:53.44 CS.seek 7ml @17:47:58.88 CSV.dial CTV @17:47:58.91
CTV.dial CSV @17:47:59.33 CBV.dial :waste2b @17:48:00.38 CS.seek 0ml @17:48:16.78 Disinfecting external Intake with kill @17:48:16.90 CSV.dial CSR @17:48:17.38 Pulling 8ml of CSR.kill @17:48:17.39 CSR.select :kill @17:48:17.50 CS.seek 8ml @17:48:37.22 delay 5 seconds @17:48:42.24 CSR.select :air @17:48:42.35 CS.seek 10ml @17:48:47.91 CSV.dial CBV @17:48:47.96
CBV.dial CSV @17:48:48.95 CTV.dial :intake @17:48:49.70 Intake.select :open @17:48:49.81 CS.seek 2ml @17:49:08.65 Intake.select :close @17:49:08.75 CTV.dial :air @17:49:09.52 CS.reconfigure :default @17:49:09.56 CS.seek 0ml @17:49:15.53 CSV.dial CSR @17:49:16.70 Pulling 5ml of CSR.flush CSR.select :flush @17:49:16.81 CS.seek 5ml @17:49:30.59 delay 5 seconds @17:49:35.61 CSR.select :air @17:49:35.72 CS.seek 10ml @17:49:49.40 CSV.dial CBV @17:49:50.38 CS.seek 0ml @17:50:16.78 Garaging FlushPuck in Collection Clamp @17:50:16.85 Elbow.reconfigure :tight @17:50:16.89 Elbow.seek CC @17:50:17.33 Elbow.reconfigure :loose @17:50:17.42 Forearm.seek CC @17:50:18.40 Hand.close @17:50:19.04 CC.seek :open @17:50:23.67 Forearm.seek :retract @17:50:24.62 Elbow.reconfigure :tight @17:50:24.66 Elbow.seek :garage @17:50:27.58 Elbow.reconfigure :loose @17:50:27.67 Forearm.reconfigure :tight @17:50:27.71 Forearm.seek :garage @17:50:28.71 Hand.open @17:50:29.35 Forearm.reconfigure :loose @17:50:29.39 Forearm.seek :retract @17:50:30.42 Completed [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] begun 15:18:36 @17:50:30.47 Asleep after completing DA_WCR_121322 assays @17:50:30.49 GoodNews.email "{1 => 28,\n 2 => 5,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@17:47:45, 17% flow, 18.7C, 25% humidity, 14.7psia, 13.734V, 0.496A, 0.478A avg, 6.81W\nSampled 1000.0ml",Subject: "Asleep after completing DA_WCR_121322 assays" @17:50:36.56 upsync @13:18:56.45UTC20-Dec-22
ESP2 v3.41 I2C Gateway v3.20 for core bus @13:18:59.12 Configuration for short puck jake 1/11/21 by brent@mbari.org /home/esp/esp2/type/shallow/jake/configure.rb @13:19:03.15 Polling Can every 10:00 @13:19:03.22 Cleanly exited at 13:16:03UTC20-Dec-22 @13:19:03.25 Loading /home/esp/esp2/mission/DA_WCR_122022.rb mission @13:19:03.30 Preparing to sleep until + 00:00:05 @13:19:03.32 GoodNews.email "Starting DA_WCR_122022 w/pucks in tube 2\n{1 => 28,\n 2 => 5,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@13:19:03, 20% flow, 13.0C, 30% humidity, 14.5psia, 13.783V, 0.284A, 0.266A avg, 3.92W",Subject: "DA_WCR_122022 begun" @13:19:03.36
Asleep til + 00:00:05 @13:19:03.40 Continuing at 13:19 @13:19:03.64 Gate.power :core,:OFF @13:19:03.66 Gate.power :raw,:OFF Shutdown @13:19:07.96 GoodNews.email "Then, in tube 2: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2/mission/DA_WCR_122022.rb:14\n{1 => 28,\n 2 => 5,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@13:19:03, 20% flow, 13.0C, 30% humidity, 14.5psia, 13.783V, 0.284A, 0.266A avg, 3.92W",Subject: "Asleep until 13:19" @13:19:08.28
Powering core bus @13:19:09.87 upsync @13:19:13.79
Gate.power :raw,:ON @13:19:14.00 upsync @13:19:14.47
CS.configure :default @13:19:14.56 PS.configure :default @13:19:14.65 SS.configure :default @13:19:14.74 CC.configure :default @13:19:14.83 PC.configure :default @13:19:14.94 Forearm.configure :loose @13:19:15.03 Elbow.configure :loose @13:19:15.14 SC.configure :loose @13:19:15.22 SE.configure :default @13:19:15.26 CH.coast @13:19:15.31 CH.configure :default @13:19:15.34 PH.coast @13:19:15.40 PH.configure :default @13:19:15.43 All dwarf controllers running firmware version 3.85 @13:19:15.54 Valve.reset @13:19:15.79 Intake.select :close @13:19:15.81 Exhaust.select :close @13:19:15.84 CTV.dial :air @13:19:15.93 PSV.dial :air @13:19:16.04 CSV.dial :waste1 @13:19:16.06
Forearm.reconfigure :loose @13:19:16.15 Forearm.home.seek :retract @13:19:16.53 SSV.dial :spit,avoiding: :exhaust @13:19:16.91 Hand.close @13:19:16.94
Elbow.jog 1500 @13:19:17.04 PS.reconfigure :default @13:19:17.09 PS.home.seek 0ml @13:19:17.37 CS.reconfigure :fast @13:19:17.40 SS.reconfigure :default @13:19:17.48 CS.home.seek 0ml @13:19:17.51 SS.home.seek 0ml @13:19:17.63
Elbow.reconfigure :loose @13:19:17.67 Elbow.home.seek :clear @13:19:22.40 SE.reconfigure :default @13:19:22.45 SE.home.seek :down @13:19:23.11 SC.reconfigure :tight @13:19:23.15 SC.home.seek :initial @13:19:25.37 PS.stop @13:19:51.48
SC.configure :limp @13:19:51.53 Forearm.seek :retract @13:19:51.58 Elbow.seek :hammer @13:19:52.63 Forearm.reconfigure :tight @13:19:52.67 Forearm.seek retracted @13:19:52.90 PC.reconfigure :default @13:19:52.92
CC.reconfigure :default @13:19:53.00 PC.home.seek :open @13:19:53.02
CC.home.seek :open @13:19:56.55 Phase [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] @13:19:56.56 Notice.email "At 13:19:56 began in tube 2: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2/mission/DA_WCR_122022.rb:14\n{1 => 28,\n 2 => 5,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@13:19:56, 21% flow, 13.0C, 30% humidity, 14.5psia, 13.773V, 0.345A, 0.339A avg, 4.75W",Subject: "Started DA_WCR_122022 phase initialPurge; da [1000, 1000] #da and wsr are run simultanelously" @13:19:56.59
Starting initialPurge @13:19:56.61 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @13:19:56.67 Hand.open @13:19:57.35 Elbow.reconfigure :tight @13:19:57.40 Elbow.seek FlushPuck::Garage @13:19:59.40 Elbow.reconfigure :loose @13:19:59.50 Forearm.reconfigure :loose @13:19:59.54 Forearm.seek :garage @13:20:00.50 Hand.close @13:20:01.22 Forearm.seek :retract @13:20:02.12 upsync @13:20:02.19
Elbow.reconfigure :tight @13:20:02.24 Elbow.seek CC @13:20:05.29 Elbow.reconfigure :loose @13:20:05.39 Forearm.reconfigure :tight @13:20:05.43 Forearm.seek CC @13:20:06.65 CC.seek :closed @13:20:11.21 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.40 @13:20:11.29 Hand.open @13:20:11.90 Forearm.reconfigure :loose @13:20:11.95 Forearm.seek :retract @13:20:12.89 Flushing the Collection Valve::Manifold @13:20:13.01 CSV.dial CSR @13:20:14.36 Pulling 4.999ml of CSR.flush @13:20:14.37 CSR.select :flush @13:20:14.48 CS.reconfigure :default @13:20:14.52 CS.seek 5ml @13:20:28.11 delay 5 seconds @13:20:33.13 CSR.select :air @13:20:33.28 CS.reconfigure :fast @13:20:33.32 CS.seek 7.499ml @13:20:40.10 CSV.dial :waste @13:20:41.38 CS.reconfigure :default @13:20:41.42 CS.seek 0ml @13:21:01.61 Purging external Intake @13:21:01.72 CSV.dial :flush @13:21:02.28 CS.reconfigure :fast @13:21:02.32 CS.seek 10ml @13:21:26.60 delay 5 seconds @13:21:31.63 CSV.dial CBV @13:21:31.65
CBV.dial CSV @13:21:32.39 CTV.dial :intake @13:21:33.20 Intake.select :open @13:21:33.30 CS.seek 0ml @13:21:56.70 Intake.select :close @13:21:56.80 CTV.dial :air @13:21:57.59 Flushing upper manifold @13:21:57.70 CSV.dial :flush @13:21:58.44 CS.seek 5ml @13:22:10.98 delay 5 seconds @13:22:16.00 CSV.dial :air @13:22:16.62 CS.seek 7ml @13:22:22.14 CSV.dial CBV @13:22:22.18
CBV.dial CSV @13:22:23.17 CTV.dial :air @13:22:23.19 CS.seek 0ml @13:22:40.10 delay 7 seconds @13:22:47.13 CS.seek 3ml @13:22:55.07 CSV.dial :waste @13:22:55.52 CS.seek 0ml @13:23:03.58 CSV.dial :flush @13:23:04.14 CS.seek 3ml @13:23:12.07 delay 5 seconds @13:23:17.09 CSV.dial :air @13:23:17.62 CS.seek 5ml @13:23:23.32 CSV.dial :air @13:23:23.34 CS.seek 0ml @13:23:35.63 delay 7 seconds @13:23:42.66 CS.seek 3ml @13:23:50.54 CSV.dial :waste @13:23:51.28 CS.seek 0ml @13:23:59.11 Garaging FlushPuck in Collection Clamp @13:23:59.17 Elbow.reconfigure :tight @13:23:59.21 Elbow.seek CC @13:23:59.52 Elbow.reconfigure :loose @13:23:59.61 Forearm.seek CC @13:24:00.56 Hand.close @13:24:01.22 CC.seek :open @13:24:05.84 Forearm.seek :retract @13:24:06.89 Elbow.reconfigure :tight @13:24:06.93 Elbow.seek :garage @13:24:09.94 Elbow.reconfigure :loose @13:24:10.03 Forearm.reconfigure :tight @13:24:10.08 Forearm.seek :garage @13:24:10.99 Hand.open @13:24:11.72 Forearm.reconfigure :loose @13:24:11.77 Forearm.seek :retract @13:24:12.74 da sampling at most 1000ml, wcr at most 1000ml @13:24:12.77 DAfans.email "Started da in tube 2 at 13:24:12UTC20-Dec-22\n{1 => 28,\n 2 => 5,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@13:19:56, 21% flow, 13.0C, 30% humidity, 14.5psia, 13.773V, 0.345A, 0.339A avg, 4.75W",Subject: "da sampling at most 1000ml, wcr at most 1000ml" @13:24:12.80
Loading sh1 puck from tube 2 into Collection Clamp @13:24:13.03 SC.reconfigure :tight @13:24:13.07 SC.seek 2 @13:24:18.38 upsync @13:24:19.84
SC.configure :limp @13:24:20.05 Elbow.reconfigure :tight @13:24:20.09 Elbow.seek 2 @13:24:22.16 Forearm.reconfigure :tight @13:24:22.21 Forearm.seek 2 + 450 counts @13:24:23.22 SE.reconfigure :pick @13:24:23.27 SE.seek :beamBreak @13:24:26.17 SE.configure :default @13:24:26.32 Elbow.seek 2 @13:24:26.61 Forearm.seek 2 + 300 counts @13:24:26.79 Forearm.reconfigure :loose @13:24:26.84 Hand.close @13:24:27.48 SE.seek :down @13:24:27.69
Forearm.seek :retract @13:24:28.65 Elbow.seek CC @13:24:29.99 SE.stop @13:24:30.31
Elbow.reconfigure :loose @13:24:30.40 Forearm.reconfigure :tight @13:24:30.44 Forearm.seek CC @13:24:31.68 CC.seek :closed @13:24:36.19 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.17 @13:24:36.20 Hand.open @13:24:36.90 Forearm.reconfigure :loose @13:24:36.94 Forearm.seek :retract @13:24:37.89 collectSample 1000ml w/bubble point of 24psi @13:24:37.90 Priming 7ml @13:24:37.91 Intake.select :close @13:24:37.92 Exhaust.select :close @13:24:37.93 SS.hold @13:24:38.13 Calibrating pressure and temperature sensors @13:24:38.16 CTV.dial :air @13:24:38.17 SSV.dial :spit,avoiding: :exhaust @13:24:38.19 SS.reconfigure :fast @13:24:38.23 SS.seek 5ml @13:24:41.08 SSV.dial CBV @13:24:41.11
CBV.dial SSV @13:24:42.43 delay 3.8 seconds @13:24:46.34 SSV.dial :spit @13:24:47.67 SS.seek 0ml @13:24:50.22 SSV.dial CBV @13:24:51.52 CTV.dial :intake @13:24:52.31 SSV.dial CBV @13:24:52.33
CBV.dial SSV @13:24:52.34 Allowing 40s for 7.0ml of remaining 7.0ml @13:24:52.35 Intake.select :open @13:24:54.51 Intake @15.2psia, vacuum from 9.7 to 12.7psi @13:24:54.52 SS.reconfigure :inhaling 2.20ml/s @13:24:54.56 SS.seek 7ml @13:25:01.00 Stroke completed -- waiting for pressure to equalize ... @13:25:01.01 SS.reconfigure :exhale @13:25:01.05 SS.hold @13:25:04.51 Intake.select :close @13:25:04.64 SSV.dial :exhaust @13:25:05.40 Exhaust.select :open @13:25:05.51 SS.seek 0ml @13:25:09.53 Exhaust.select :close @13:25:09.64 Sampling 1000.0ml Intake.select :close @13:25:09.65 Exhaust.select :close @13:25:09.67 SS.reconfigure :default @13:25:09.71 SS.hold @13:25:09.89 CTV.dial :intake @13:25:09.90 SSV.dial CBV @13:25:09.92
CBV.dial SSV @13:25:10.70 Allowing 130s for 25.0ml of remaining 1000.0ml @13:25:10.71 Intake.select :open @13:25:12.85 Intake @15.6psia, vacuum from 10.0 to 13.0psi @13:25:12.87 SS.reconfigure :inhaling 2.20ml/s @13:25:12.91 SS.seek 25ml @13:25:21.70 Vacuum too high -- waiting up to 40 seconds @13:25:21.71 SS.reconfigure :exhale @13:25:21.75 SS.hold @13:25:22.90 Intake @12.4psia, vacuum from 6.9 to 9.9psi @13:25:22.91 SS.reconfigure :inhaling 1.98ml/s @13:25:22.96 SS.seek 25ml @13:25:27.48 Vacuum too high -- waiting up to 40 seconds @13:25:27.49 SS.reconfigure :exhale @13:25:27.53 SS.hold @13:25:33.39 Intake @13.8psia, vacuum from 8.3 to 11.3psi @13:25:33.41 SS.reconfigure :inhaling 1.79ml/s @13:25:33.45 SS.seek 25ml @13:25:37.14 Stroke completed -- waiting for pressure to equalize ... @13:25:37.15 SS.reconfigure :exhale @13:25:37.19 SS.hold @13:25:39.15 Intake.select :close @13:25:39.29 SSV.dial :exhaust @13:25:40.05 Exhaust.select :open @13:25:40.16 SS.seek 0ml @13:25:48.83 Exhaust.select :close @13:25:48.93 SSV.dial CBV @13:25:48.95
CBV.dial SSV @13:25:49.72 Allowing 130s for 25.0ml of remaining 975.0ml @13:25:49.73 Intake.select :open @13:25:51.89 Intake @15.4psia, vacuum from 9.9 to 12.9psi @13:25:51.90 SS.reconfigure :inhaling 1.79ml/s @13:25:51.94 SS.seek 25ml @13:26:04.70 Vacuum too high -- waiting up to 40 seconds @13:26:04.71 SS.reconfigure :exhale @13:26:04.75 SS.hold @13:26:05.33 Intake @12.4psia, vacuum from 6.9 to 9.9psi @13:26:05.34 SS.reconfigure :inhaling 1.61ml/s @13:26:05.39 SS.seek 25ml @13:26:09.20 Vacuum too high -- waiting up to 40 seconds @13:26:09.21 SS.reconfigure :exhale @13:26:09.25 SS.hold @13:26:15.90 Intake @13.7psia, vacuum from 8.2 to 11.2psi @13:26:15.91 SS.reconfigure :inhaling 1.45ml/s @13:26:15.95 SS.seek 25ml @13:26:18.20 Stroke completed -- waiting for pressure to equalize ... @13:26:18.21 SS.reconfigure :exhale @13:26:18.25 SS.hold @13:26:20.66 Intake.select :close @13:26:20.80 SSV.dial :exhaust @13:26:21.55 Exhaust.select :open @13:26:21.65 SS.seek 0ml @13:26:30.28 Exhaust.select :close @13:26:30.38 SSV.dial CBV @13:26:30.41
CBV.dial SSV @13:26:31.17 Allowing 130s for 25.0ml of remaining 950.0ml @13:26:31.18 Intake.select :open @13:26:33.34 Intake @15.4psia, vacuum from 9.9 to 12.9psi @13:26:33.36 SS.reconfigure :inhaling 1.45ml/s @13:26:33.40 SS.seek 25ml @13:26:53.59 Stroke completed -- waiting for pressure to equalize ... @13:26:53.60 SS.reconfigure :exhale @13:26:53.64 SS.hold @13:27:02.10 Intake.select :close @13:27:02.24 SSV.dial :exhaust @13:27:02.99 Exhaust.select :open @13:27:03.09 SS.seek 0ml @13:27:12.06 Exhaust.select :close @13:27:12.16 SSV.dial CBV @13:27:12.19
CBV.dial SSV @13:27:12.96 Allowing 130s for 25.0ml of remaining 925.0ml @13:27:12.97 Intake.select :open @13:27:15.12 Intake @15.4psia, vacuum from 9.9 to 12.9psi @13:27:15.14 SS.reconfigure :inhaling 1.45ml/s @13:27:15.18 SS.seek 25ml @13:27:33.60 Vacuum too high -- waiting up to 40 seconds @13:27:33.61 SS.reconfigure :exhale @13:27:33.65 SS.hold @13:27:34.42 Intake @12.5psia, vacuum from 7.0 to 10.0psi @13:27:34.43 SS.reconfigure :inhaling 1.30ml/s @13:27:34.48 SS.seek 25ml @13:27:36.44 Stroke completed -- waiting for pressure to equalize ... @13:27:36.45 SS.reconfigure :exhale @13:27:36.49 SS.hold @13:27:43.96 Intake.select :close @13:27:44.10 SSV.dial :exhaust @13:27:44.86 Exhaust.select :open @13:27:44.97 SS.seek 0ml @13:27:53.73 Exhaust.select :close @13:27:53.84 SSV.dial CBV @13:27:53.89
CBV.dial SSV @13:27:54.61 Allowing 130s for 25.0ml of remaining 900.0ml @13:27:54.62 Intake.select :open @13:27:56.77 Intake @15.4psia, vacuum from 9.9 to 12.9psi @13:27:56.79 SS.reconfigure :inhaling 1.30ml/s @13:27:56.83 SS.seek 25ml @13:28:18.97 Stroke completed -- waiting for pressure to equalize ... @13:28:18.98 SS.reconfigure :exhale @13:28:19.02 SS.hold @13:28:27.55 Intake.select :close @13:28:27.69 SSV.dial :exhaust @13:28:28.45 Exhaust.select :open @13:28:28.56 SS.seek 0ml @13:28:37.33 Exhaust.select :close @13:28:37.43 SSV.dial CBV @13:28:37.45
CBV.dial SSV @13:28:38.22 Allowing 130s for 25.0ml of remaining 875.0ml @13:28:38.23 Intake.select :open @13:28:40.38 Intake @15.5psia, vacuum from 10.0 to 13.0psi @13:28:40.40 SS.reconfigure :inhaling 1.30ml/s @13:28:40.44 SS.seek 25ml @13:29:02.39 Stroke completed -- waiting for pressure to equalize ... @13:29:02.40 SS.reconfigure :exhale @13:29:02.44 SS.hold @13:29:10.97 Intake.select :close @13:29:11.11 SSV.dial :exhaust @13:29:11.87 Exhaust.select :open @13:29:11.98 SS.seek 0ml @13:29:20.65 Exhaust.select :close @13:29:20.76 SSV.dial CBV @13:29:20.81
CBV.dial SSV @13:29:21.53 Allowing 130s for 25.0ml of remaining 850.0ml @13:29:21.54 Intake.select :open @13:29:23.69 Intake @15.4psia, vacuum from 9.9 to 12.9psi @13:29:23.71 SS.reconfigure :inhaling 1.30ml/s @13:29:23.75 SS.seek 25ml @13:29:44.75 Vacuum too high -- waiting up to 40 seconds @13:29:44.76 SS.reconfigure :exhale @13:29:44.81 SS.hold @13:29:46.53 Intake @12.9psia, vacuum from 7.4 to 10.4psi @13:29:46.54 SS.reconfigure :inhaling 1.17ml/s @13:29:46.59 SS.seek 25ml @13:29:47.98 Stroke completed -- waiting for pressure to equalize ... @13:29:47.99 SS.reconfigure :exhale @13:29:48.03 SS.hold @13:29:55.40 Intake.select :close @13:29:55.54 SSV.dial :exhaust @13:29:56.29 Exhaust.select :open @13:29:56.40 SS.seek 0ml @13:30:05.35 Exhaust.select :close @13:30:05.46 SSV.dial CBV @13:30:05.48
CBV.dial SSV @13:30:06.25 Allowing 130s for 25.0ml of remaining 825.0ml @13:30:06.26 Intake.select :open @13:30:08.41 Intake @15.4psia, vacuum from 9.9 to 12.9psi @13:30:08.43 SS.reconfigure :inhaling 1.17ml/s @13:30:08.47 SS.seek 25ml @13:30:32.10 Stroke completed -- waiting for pressure to equalize ... @13:30:32.11 SS.reconfigure :exhale @13:30:32.16 SS.hold @13:30:41.84 Intake.select :close @13:30:41.98 SSV.dial :exhaust @13:30:42.75 Exhaust.select :open @13:30:42.86 SS.seek 0ml @13:30:51.57 Exhaust.select :close @13:30:52.17 SSV.dial CBV @13:30:52.19
CBV.dial SSV @13:30:52.95 Allowing 130s for 25.0ml of remaining 800.0ml @13:30:52.96 Intake.select :open @13:30:55.11 Intake @15.4psia, vacuum from 9.9 to 12.9psi @13:30:55.13 SS.reconfigure :inhaling 1.17ml/s @13:30:55.17 SS.seek 25ml @13:31:19.12 Stroke completed -- waiting for pressure to equalize ... @13:31:19.13 SS.reconfigure :exhale @13:31:19.17 SS.hold @13:31:28.92 Intake.select :close @13:31:29.06 SSV.dial :exhaust @13:31:29.81 Exhaust.select :open @13:31:29.92 SS.seek 0ml @13:31:38.51 Exhaust.select :close @13:31:38.62 SSV.dial CBV @13:31:38.67
CBV.dial SSV @13:31:39.39 Allowing 130s for 25.0ml of remaining 775.0ml @13:31:39.40 Intake.select :open @13:31:41.55 Intake @15.4psia, vacuum from 9.9 to 12.9psi @13:31:41.57 SS.reconfigure :inhaling 1.17ml/s @13:31:41.61 SS.seek 25ml @13:32:03.47 Vacuum too high -- waiting up to 40 seconds @13:32:03.48 SS.reconfigure :exhale @13:32:03.52 SS.hold @13:32:07.69 Intake @13.6psia, vacuum from 8.1 to 11.1psi @13:32:07.71 SS.reconfigure :inhaling 1.06ml/s @13:32:07.75 SS.seek 25ml @13:32:10.49 Stroke completed -- waiting for pressure to equalize ... @13:32:10.50 SS.reconfigure :exhale @13:32:10.55 SS.hold @13:32:17.03 Intake.select :close @13:32:17.17 SSV.dial :exhaust @13:32:17.94 Exhaust.select :open @13:32:18.04 SS.seek 0ml @13:32:26.73 Exhaust.select :close @13:32:26.83 SSV.dial CBV @13:32:26.85
CBV.dial SSV @13:32:27.62 Allowing 130s for 25.0ml of remaining 750.0ml @13:32:27.63 Intake.select :open @13:32:29.78 Intake @15.5psia, vacuum from 10.0 to 13.0psi @13:32:29.80 SS.reconfigure :inhaling 1.06ml/s @13:32:29.84 SS.seek 25ml @13:32:56.27 Stroke completed -- waiting for pressure to equalize ... @13:32:56.28 SS.reconfigure :exhale @13:32:56.32 SS.hold @13:33:05.98 Intake.select :close @13:33:06.12 SSV.dial :exhaust @13:33:06.86 Exhaust.select :open @13:33:06.97 SS.seek 0ml @13:33:16.04 Exhaust.select :close @13:33:16.14 SSV.dial CBV @13:33:16.16
CBV.dial SSV @13:33:16.92 Allowing 130s for 25.0ml of remaining 725.0ml @13:33:16.93 Intake.select :open @13:33:19.08 Intake @15.5psia, vacuum from 10.0 to 13.0psi @13:33:19.09 SS.reconfigure :inhaling 1.06ml/s @13:33:19.14 SS.seek 25ml @13:33:41.25 Vacuum too high -- waiting up to 40 seconds @13:33:41.26 SS.reconfigure :exhale @13:33:41.30 SS.hold @13:33:44.88 Intake @13.8psia, vacuum from 8.3 to 11.3psi @13:33:44.90 SS.reconfigure :inhaling 0.95ml/s @13:33:44.94 SS.seek 25ml @13:33:49.27 Stroke completed -- waiting for pressure to equalize ... @13:33:49.28 SS.reconfigure :exhale @13:33:49.32 SS.hold @13:33:56.81 Intake.select :close @13:33:56.95 SSV.dial :exhaust @13:33:57.71 Exhaust.select :open @13:33:57.82 SS.seek 0ml @13:34:06.48 Exhaust.select :close @13:34:06.58 SSV.dial CBV @13:34:06.60
CBV.dial SSV @13:34:07.37 Allowing 130s for 25.0ml of remaining 700.0ml @13:34:07.38 Intake.select :open @13:34:09.53 Intake @15.4psia, vacuum from 9.9 to 12.9psi @13:34:09.55 SS.reconfigure :inhaling 0.95ml/s @13:34:09.59 SS.seek 25ml @13:34:38.51 Stroke completed -- waiting for pressure to equalize ... @13:34:38.52 SS.reconfigure :exhale @13:34:38.56 SS.hold @13:34:49.68 Intake.select :close @13:34:49.82 SSV.dial :exhaust @13:34:50.57 Exhaust.select :open @13:34:50.68 SS.seek 0ml @13:34:59.62 Exhaust.select :close @13:34:59.72 SSV.dial CBV @13:34:59.74
CBV.dial SSV @13:35:00.51 Allowing 130s for 25.0ml of remaining 675.0ml @13:35:00.52 Intake.select :open @13:35:02.67 Intake @15.4psia, vacuum from 9.9 to 12.9psi @13:35:02.69 SS.reconfigure :inhaling 0.95ml/s @13:35:02.73 SS.seek 25ml @13:35:31.12 Stroke completed -- waiting for pressure to equalize ... @13:35:31.13 SS.reconfigure :exhale @13:35:31.17 SS.hold @13:35:43.80 Intake.select :close @13:35:43.94 SSV.dial :exhaust @13:35:44.69 Exhaust.select :open @13:35:44.80 SS.seek 0ml @13:35:53.54 Exhaust.select :close @13:35:53.64 SSV.dial CBV @13:35:53.66
CBV.dial SSV @13:35:54.43 Allowing 130s for 25.0ml of remaining 650.0ml @13:35:54.44 Intake.select :open @13:35:56.59 Intake @15.5psia, vacuum from 10.0 to 13.0psi @13:35:56.61 SS.reconfigure :inhaling 0.95ml/s @13:35:56.65 SS.seek 25ml @13:36:19.00 Vacuum too high -- waiting up to 40 seconds @13:36:19.01 SS.reconfigure :exhale @13:36:19.05 SS.hold @13:36:23.90 Intake @14.1psia, vacuum from 8.6 to 11.6psi @13:36:23.92 SS.reconfigure :inhaling 0.87ml/s @13:36:23.96 SS.seek 25ml @13:36:31.22 Stroke completed -- waiting for pressure to equalize ... @13:36:31.23 SS.reconfigure :exhale @13:36:31.27 SS.hold @13:36:39.80 Intake.select :close @13:36:39.94 SSV.dial :exhaust @13:36:40.69 Exhaust.select :open @13:36:40.80 SS.seek 0ml @13:36:49.54 Exhaust.select :close @13:36:49.64 SSV.dial CBV @13:36:49.66
CBV.dial SSV @13:36:50.43 Allowing 130s for 25.0ml of remaining 625.0ml @13:36:50.44 Intake.select :open @13:36:52.59 Intake @15.6psia, vacuum from 10.0 to 13.0psi @13:36:52.61 SS.reconfigure :inhaling 0.87ml/s @13:36:52.65 SS.seek 25ml @13:37:21.40 Vacuum too high -- waiting up to 40 seconds @13:37:21.41 SS.reconfigure :exhale @13:37:21.45 SS.hold @13:37:27.32 Intake @14.2psia, vacuum from 8.7 to 11.7psi @13:37:27.33 SS.reconfigure :inhaling 0.78ml/s @13:37:27.38 SS.seek 25ml @13:37:30.67 Stroke completed -- waiting for pressure to equalize ... @13:37:30.68 SS.reconfigure :exhale @13:37:30.73 SS.hold @13:37:38.21 Intake.select :close @13:37:38.34 SSV.dial :exhaust @13:37:39.10 Exhaust.select :open @13:37:39.21 SS.seek 0ml @13:37:48.24 Exhaust.select :close @13:37:48.34 SSV.dial CBV @13:37:48.36
CBV.dial SSV @13:37:49.13 Allowing 130s for 25.0ml of remaining 600.0ml @13:37:49.14 Intake.select :open @13:37:51.30 Intake @15.4psia, vacuum from 9.9 to 12.9psi @13:37:51.32 SS.reconfigure :inhaling 0.78ml/s @13:37:51.36 SS.seek 25ml @13:38:25.21 Stroke completed -- waiting for pressure to equalize ... @13:38:25.22 SS.reconfigure :exhale @13:38:25.26 SS.hold @13:38:39.61 Intake.select :close @13:38:39.75 SSV.dial :exhaust @13:38:40.50 Exhaust.select :open @13:38:40.61 SS.seek 0ml @13:38:49.55 Exhaust.select :close @13:38:49.65 SSV.dial CBV @13:38:49.67
CBV.dial SSV @13:38:50.44 Allowing 130s for 25.0ml of remaining 575.0ml @13:38:50.45 Intake.select :open @13:38:52.60 Intake @15.4psia, vacuum from 9.9 to 12.9psi @13:38:52.62 SS.reconfigure :inhaling 0.78ml/s @13:38:52.66 SS.seek 25ml @13:39:26.59 Stroke completed -- waiting for pressure to equalize ... @13:39:26.60 SS.reconfigure :exhale @13:39:26.65 SS.hold @13:39:40.99 Intake.select :close @13:39:41.13 SSV.dial :exhaust @13:39:41.89 Exhaust.select :open @13:39:42.00 SS.seek 0ml @13:39:50.64 Exhaust.select :close @13:39:50.74 SSV.dial CBV @13:39:50.77
CBV.dial SSV @13:39:51.53 Allowing 130s for 25.0ml of remaining 550.0ml @13:39:51.54 Intake.select :open @13:39:53.70 Intake @15.4psia, vacuum from 9.9 to 12.9psi @13:39:53.72 SS.reconfigure :inhaling 0.78ml/s @13:39:53.76 SS.seek 25ml @13:40:23.49 Vacuum too high -- waiting up to 40 seconds @13:40:23.50 SS.reconfigure :exhale @13:40:23.55 SS.hold @13:40:31.04 Intake @14.4psia, vacuum from 8.9 to 11.9psi @13:40:31.06 SS.reconfigure :inhaling 0.70ml/s @13:40:31.10 SS.seek 25ml @13:40:36.62 Stroke completed -- waiting for pressure to equalize ... @13:40:36.63 SS.reconfigure :exhale @13:40:36.68 SS.hold @13:40:45.24 Intake.select :close @13:40:45.38 SSV.dial :exhaust @13:40:46.14 Exhaust.select :open @13:40:46.25 SS.seek 0ml @13:40:55.05 Exhaust.select :close @13:40:55.15 SSV.dial CBV @13:40:55.17
CBV.dial SSV @13:40:55.94 Allowing 130s for 25.0ml of remaining 525.0ml @13:40:55.95 Intake.select :open @13:40:58.10 Intake @15.4psia, vacuum from 9.9 to 12.9psi @13:40:58.12 SS.reconfigure :inhaling 0.70ml/s @13:40:58.16 SS.seek 25ml @13:41:35.24 Vacuum too high -- waiting up to 40 seconds @13:41:35.25 SS.reconfigure :exhale @13:41:35.30 SS.hold @13:41:42.76 Intake @14.3psia, vacuum from 8.8 to 11.8psi @13:41:42.77 SS.reconfigure :inhaling 0.63ml/s @13:41:42.81 SS.seek 25ml @13:41:43.78 Stroke completed -- waiting for pressure to equalize ... @13:41:43.79 SS.reconfigure :exhale @13:41:43.83 SS.hold @13:41:51.29 Intake.select :close @13:41:51.43 SSV.dial :exhaust @13:41:52.19 Exhaust.select :open @13:41:52.30 SS.seek 0ml @13:42:01.06 Exhaust.select :close @13:42:01.16 SSV.dial CBV @13:42:01.19
CBV.dial SSV @13:42:01.95 Allowing 130s for 25.0ml of remaining 500.0ml @13:42:01.96 Intake.select :open @13:42:04.12 Intake @15.4psia, vacuum from 9.9 to 12.9psi @13:42:04.13 SS.reconfigure :inhaling 0.63ml/s @13:42:04.18 SS.seek 25ml @13:42:46.18 Stroke completed -- waiting for pressure to equalize ... @13:42:46.19 SS.reconfigure :exhale @13:42:46.24 SS.hold @13:43:02.44 Intake.select :close @13:43:02.58 SSV.dial :exhaust @13:43:03.33 Exhaust.select :open @13:43:03.44 SS.seek 0ml @13:43:12.39 Exhaust.select :close @13:43:12.49 SSV.dial CBV @13:43:12.51
CBV.dial SSV @13:43:13.29 Allowing 130s for 25.0ml of remaining 475.0ml @13:43:13.30 Intake.select :open @13:43:15.45 Intake @15.4psia, vacuum from 9.9 to 12.9psi @13:43:15.47 SS.reconfigure :inhaling 0.63ml/s @13:43:15.51 SS.seek 25ml @13:43:55.02 Vacuum too high -- waiting up to 40 seconds @13:43:55.03 SS.reconfigure :exhale @13:43:55.07 SS.hold @13:44:03.61 Intake @14.6psia, vacuum from 9.1 to 12.1psi @13:44:03.63 SS.reconfigure :inhaling 0.57ml/s @13:44:03.67 SS.seek 25ml @13:44:06.00 Stroke completed -- waiting for pressure to equalize ... @13:44:06.01 SS.reconfigure :exhale @13:44:06.05 SS.hold @13:44:15.81 Intake.select :close @13:44:15.94 SSV.dial :exhaust @13:44:16.71 Exhaust.select :open @13:44:16.81 SS.seek 0ml @13:44:25.57 Exhaust.select :close @13:44:25.68 SSV.dial CBV @13:44:25.71
CBV.dial SSV @13:44:26.47 Allowing 130s for 25.0ml of remaining 450.0ml @13:44:26.48 Intake.select :open @13:44:28.63 Intake @15.4psia, vacuum from 9.9 to 12.9psi @13:44:28.65 SS.reconfigure :inhaling 0.57ml/s @13:44:28.69 SS.seek 25ml @13:45:14.85 Stroke completed -- waiting for pressure to equalize ... @13:45:14.86 SS.reconfigure :exhale @13:45:14.91 SS.hold @13:45:33.22 Intake.select :close @13:45:33.36 SSV.dial :exhaust @13:45:34.12 Exhaust.select :open @13:45:34.23 SS.seek 0ml @13:45:42.99 Exhaust.select :close @13:45:43.10 SSV.dial CBV @13:45:43.13
CBV.dial SSV @13:45:43.87 Allowing 130s for 25.0ml of remaining 425.0ml @13:45:43.88 Intake.select :open @13:45:46.03 Intake @15.4psia, vacuum from 9.9 to 12.9psi @13:45:46.05 SS.reconfigure :inhaling 0.57ml/s @13:45:46.09 SS.seek 25ml @13:46:25.71 Vacuum too high -- waiting up to 40 seconds @13:46:25.72 SS.reconfigure :exhale @13:46:25.76 SS.hold @13:46:35.51 Intake @14.9psia, vacuum from 9.4 to 12.4psi @13:46:35.52 SS.reconfigure :inhaling 0.51ml/s @13:46:35.57 SS.seek 25ml @13:46:43.45 Stroke completed -- waiting for pressure to equalize ... @13:46:43.46 SS.reconfigure :exhale @13:46:43.50 SS.hold @13:46:54.87 Intake.select :close @13:46:55.01 SSV.dial :exhaust @13:46:55.77 Exhaust.select :open @13:46:55.87 SS.seek 0ml @13:47:04.65 Exhaust.select :close @13:47:04.75 SSV.dial CBV @13:47:04.77
CBV.dial SSV @13:47:05.54 Allowing 130s for 25.0ml of remaining 400.0ml @13:47:05.55 Intake.select :open @13:47:07.70 Intake @15.4psia, vacuum from 9.9 to 12.9psi @13:47:07.72 SS.reconfigure :inhaling 0.51ml/s @13:47:07.76 SS.seek 25ml @13:47:53.58 Vacuum too high -- waiting up to 40 seconds @13:47:53.59 SS.reconfigure :exhale @13:47:53.63 SS.hold @13:48:03.38 Intake @14.9psia, vacuum from 9.4 to 12.4psi @13:48:03.40 SS.reconfigure :inhaling 0.47ml/s @13:48:03.44 SS.seek 25ml @13:48:09.51 Stroke completed -- waiting for pressure to equalize ... @13:48:09.52 SS.reconfigure :exhale @13:48:09.56 SS.hold @13:48:22.20 Intake.select :close @13:48:22.33 SSV.dial :exhaust @13:48:23.09 Exhaust.select :open @13:48:23.19 SS.seek 0ml @13:48:31.94 Exhaust.select :close @13:48:32.05 SSV.dial CBV @13:48:32.07
CBV.dial SSV @13:48:32.84 Allowing 130s for 25.0ml of remaining 375.0ml @13:48:32.85 Intake.select :open @13:48:35.00 Intake @15.5psia, vacuum from 10.0 to 13.0psi @13:48:35.02 SS.reconfigure :inhaling 0.47ml/s @13:48:35.06 SS.seek 25ml @13:49:31.16 Stroke completed -- waiting for pressure to equalize ... @13:49:31.17 SS.reconfigure :exhale @13:49:31.21 SS.hold @13:49:53.61 Intake.select :close @13:49:53.75 SSV.dial :exhaust @13:49:54.51 Exhaust.select :open @13:49:54.61 SS.seek 0ml @13:50:03.61 Exhaust.select :close @13:50:03.72 SSV.dial CBV @13:50:03.74
CBV.dial SSV @13:50:04.51 Allowing 130s for 25.0ml of remaining 350.0ml @13:50:04.52 Intake.select :open @13:50:06.68 Intake @15.4psia, vacuum from 9.9 to 12.9psi @13:50:06.69 SS.reconfigure :inhaling 0.47ml/s @13:50:06.74 SS.seek 25ml @13:50:56.45 Vacuum too high -- waiting up to 40 seconds @13:50:56.46 SS.reconfigure :exhale @13:50:56.50 SS.hold @13:51:08.14 Intake @15.0psia, vacuum from 9.5 to 12.5psi @13:51:08.15 SS.reconfigure :inhaling 0.43ml/s @13:51:08.20 SS.seek 25ml @13:51:15.10 Stroke completed -- waiting for pressure to equalize ... @13:51:15.11 SS.reconfigure :exhale @13:51:15.15 SS.hold @13:51:31.44 Intake.select :close @13:51:31.58 SSV.dial :exhaust @13:51:32.33 Exhaust.select :open @13:51:32.44 SS.seek 0ml @13:51:41.19 Exhaust.select :close @13:51:41.29 SSV.dial CBV @13:51:41.32
CBV.dial SSV @13:51:42.07 Allowing 130s for 25.0ml of remaining 325.0ml @13:51:42.08 Intake.select :open @13:51:44.23 Intake @15.4psia, vacuum from 9.9 to 12.9psi @13:51:44.25 SS.reconfigure :inhaling 0.43ml/s @13:51:44.29 SS.seek 25ml @13:52:45.10 Vacuum too high -- waiting up to 40 seconds @13:52:45.11 SS.reconfigure :exhale @13:52:45.16 SS.hold @13:52:57.68 Intake @14.9psia, vacuum from 9.4 to 12.4psi @13:52:57.70 SS.reconfigure :inhaling 0.38ml/s @13:52:57.74 SS.seek 25ml @13:52:58.56 Stroke completed -- waiting for pressure to equalize ... @13:52:58.57 SS.reconfigure :exhale @13:52:58.62 SS.hold @13:53:11.26 Intake.select :close @13:53:11.40 SSV.dial :exhaust @13:53:12.15 Exhaust.select :open @13:53:12.26 SS.seek 0ml @13:53:21.05 Exhaust.select :close @13:53:21.15 SSV.dial CBV @13:53:21.17
CBV.dial SSV @13:53:21.93 Allowing 130s for 25.0ml of remaining 300.0ml @13:53:21.94 Intake.select :open @13:53:24.09 Intake @15.4psia, vacuum from 9.9 to 12.9psi @13:53:24.11 SS.reconfigure :inhaling 0.38ml/s @13:53:24.15 SS.seek 25ml @13:54:32.72 Stroke completed -- waiting for pressure to equalize ... @13:54:32.73 SS.reconfigure :exhale @13:54:32.77 SS.hold @13:54:57.55 Intake.select :close @13:54:57.68 SSV.dial :exhaust @13:54:58.42 Exhaust.select :open @13:54:58.53 SS.seek 0ml @13:55:07.22 Exhaust.select :close @13:55:07.32 SSV.dial CBV @13:55:07.34
CBV.dial SSV @13:55:08.10 Allowing 130s for 25.0ml of remaining 275.0ml @13:55:08.11 Intake.select :open @13:55:10.27 Intake @15.5psia, vacuum from 10.0 to 13.0psi @13:55:10.28 SS.reconfigure :inhaling 0.38ml/s @13:55:10.33 SS.seek 25ml @13:56:17.13 Vacuum too high -- waiting up to 40 seconds @13:56:17.14 SS.reconfigure :exhale @13:56:17.18 SS.hold @13:56:31.44 Intake @15.0psia, vacuum from 9.5 to 12.5psi @13:56:31.45 SS.reconfigure :inhaling 0.34ml/s @13:56:31.50 SS.seek 25ml @13:56:34.28 Stroke completed -- waiting for pressure to equalize ... @13:56:34.29 SS.reconfigure :exhale @13:56:34.33 SS.hold @13:56:48.68 Intake.select :close @13:56:48.82 SSV.dial :exhaust @13:56:49.58 Exhaust.select :open @13:56:49.68 SS.seek 0ml @13:56:58.64 Exhaust.select :close @13:56:58.74 SSV.dial CBV @13:56:58.76
CBV.dial SSV @13:56:59.53 Allowing 130s for 25.0ml of remaining 250.0ml @13:56:59.54 Intake.select :open @13:57:01.69 Intake @15.4psia, vacuum from 9.9 to 12.9psi @13:57:01.71 SS.reconfigure :inhaling 0.34ml/s @13:57:01.75 SS.seek 25ml @13:58:21.54 Stroke completed -- waiting for pressure to equalize ... @13:58:21.55 SS.reconfigure :exhale @13:58:21.59 SS.hold @13:58:48.55 Intake.select :close @13:58:48.69 SSV.dial :exhaust @13:58:49.45 Exhaust.select :open @13:58:49.56 SS.seek 0ml @13:58:58.40 Exhaust.select :close @13:58:58.51 SSV.dial CBV @13:58:58.55
CBV.dial SSV @13:58:59.30 Allowing 130s for 25.0ml of remaining 225.0ml @13:58:59.31 Intake.select :open @13:59:01.46 Intake @15.4psia, vacuum from 9.9 to 12.9psi @13:59:01.48 SS.reconfigure :inhaling 0.34ml/s @13:59:01.52 SS.seek 25ml @14:00:21.54 Stroke completed -- waiting for pressure to equalize ... @14:00:21.55 SS.reconfigure :exhale @14:00:21.59 SS.hold @14:00:54.35 Intake.select :close @14:00:54.51 SSV.dial :exhaust @14:00:55.27 Exhaust.select :open @14:00:55.38 SS.seek 0ml @14:01:04.35 Exhaust.select :close @14:01:04.46 SSV.dial CBV @14:01:04.51
CBV.dial SSV @14:01:05.23 Allowing 130s for 25.0ml of remaining 200.0ml @14:01:05.24 Intake.select :open @14:01:07.40 Intake @15.4psia, vacuum from 9.9 to 12.9psi @14:01:07.41 SS.reconfigure :inhaling 0.34ml/s @14:01:07.46 SS.seek 25ml @14:02:03.06 Vacuum too high -- waiting up to 40 seconds @14:02:03.07 SS.reconfigure :exhale @14:02:03.12 SS.hold @14:02:17.36 Intake @15.2psia, vacuum from 9.7 to 12.7psi @14:02:17.37 SS.reconfigure :inhaling 0.31ml/s @14:02:17.42 SS.seek 25ml @14:02:44.09 Stroke completed -- waiting for pressure to equalize ... @14:02:44.10 SS.reconfigure :exhale @14:02:44.14 SS.hold @14:03:12.69 Intake.select :close @14:03:12.82 SSV.dial :exhaust @14:03:13.59 Exhaust.select :open @14:03:13.69 SS.seek 0ml @14:03:22.47 Exhaust.select :close @14:03:22.58 SSV.dial CBV @14:03:22.60
CBV.dial SSV @14:03:23.82 Allowing 130s for 25.0ml of remaining 175.0ml @14:03:23.83 Intake.select :open @14:03:25.98 Intake @15.4psia, vacuum from 9.9 to 12.9psi @14:03:26.00 SS.reconfigure :inhaling 0.31ml/s @14:03:26.05 SS.seek 25ml @14:04:37.03 Vacuum too high -- waiting up to 40 seconds @14:04:37.04 SS.reconfigure :exhale @14:04:37.08 SS.hold @14:04:55.29 Intake @15.2psia, vacuum from 9.7 to 12.7psi @14:04:55.30 SS.reconfigure :inhaling 0.28ml/s @14:04:55.35 SS.seek 25ml @14:05:15.04 Stroke completed -- waiting for pressure to equalize ... SS.reconfigure :exhale @14:05:15.09 SS.hold @14:05:33.84 SS.reconfigure :default @14:05:33.88 SS.hold @14:05:33.98 Intake.select :close @14:05:34.13 ShallowSampler::Clogged in MAIN -- Filtering too Slowly after 850.0ml @14:05:34.14 SSV.dial :exhaust @14:05:34.88 Exhaust.select :open @14:05:34.99 SS.reconfigure :exhale @14:05:35.03 SS.seek 0ml @14:05:43.82 Exhaust.select :close @14:05:43.92 Sampled 850.0ml @14:05:43.93 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @14:05:43.94 Intake.select :close @14:05:44.00 CSV.dial :waste @14:05:44.06 CS.seek 0ml @14:05:44.14 CSV.dial :air @14:05:44.17 SSV.dial CBV,avoiding: :exhaust @14:05:44.21
CBV.dial SSV @14:05:44.95 CS.seek 10ml @14:05:46.10
CTV.dial :air @14:05:46.90 delay 15 seconds @14:06:01.92 SSV.dial :spit,avoiding: :exhaust @14:06:03.23 CTV.dial CSV,avoiding: :intake @14:06:03.26
SS.seek 0ml @14:06:03.37 SSV.dial CBV,avoiding: :exhaust @14:06:09.34 CBV.dial between SSV and waste2 @14:06:09.73 CTV.dial CSV,avoiding: :air @14:06:09.75
CSV.dial CTV @14:06:10.18 CS.reconfigure :pressurize @14:06:10.23 CS.seek 0ml @14:06:28.79 BubblePoint: Intake @38.9psia, Outlet @14.5psia @14:06:28.80 CBV.dial SSV @14:06:29.12 SS.reconfigure :default @14:06:29.17 SS.seek 10ml @14:06:34.77 delay 15 seconds @14:06:49.88 Evacuated: Intake @16.3psia, Outlet @9.5psia @14:06:49.89 SSV.dial :spit,avoiding: :exhaust @14:06:51.23 SS.reconfigure :exhale @14:06:51.27 SS.seek 0ml @14:06:56.11 SS.reconfigure :fast @14:06:56.15 SS.seek 5ml @14:06:58.95 SSV.dial CBV,avoiding: :exhaust @14:06:58.98
CBV.dial SSV @14:07:00.27 CTV.dial :air,avoiding: :intake @14:07:01.63 CSV.dial CTV @14:07:01.67
SS.seek 10ml @14:07:02.66 CSV.dial :air @14:07:03.12 CS.reconfigure :fast @14:07:03.17 CS.seek 0ml @14:07:04.13
SSV.dial :spit,avoiding: :exhaust @14:07:05.92 SS.reconfigure :exhale @14:07:05.96 SS.seek 0ml @14:07:10.66 CS.stop @14:07:10.90
SS.reconfigure :fast @14:07:10.95 SS.seek 5ml @14:07:13.75 SSV.dial CBV,avoiding: :exhaust @14:07:13.77
CBV.dial SSV @14:07:16.09 SSV.dial :spit,avoiding: :exhaust @14:07:17.40 SS.reconfigure :default @14:07:17.44 SS.seek 0ml @14:07:20.63 SS.stop @14:07:20.67 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) Intake.select :close @14:07:20.74 CSV.dial :waste @14:07:20.85 SSV.dial CBV,avoiding: :exhaust @14:07:20.87
CBV.dial SSV @14:07:21.53 CS.seek 0ml @14:07:21.60 CSV.dial :air @14:07:22.20
CTV.dial :air @14:07:22.21 delay 15 seconds @14:07:22.32 CS.seek 10ml @14:07:37.23
SSV.dial :spit,avoiding: :exhaust @14:07:38.56 CTV.dial CSV,avoiding: :intake @14:07:38.59
SS.reconfigure :exhale @14:07:38.68 SS.seek 0ml @14:07:39.11 SSV.dial CBV,avoiding: :exhaust @14:07:46.65 CBV.dial between SSV and waste2 @14:07:47.05 CTV.dial CSV,avoiding: :air @14:07:47.07
CSV.dial CTV @14:07:47.52 CS.reconfigure :pressurize @14:07:47.57 CS.seek 0ml @14:08:08.82 BubblePoint: Intake @37.7psia, Outlet @13.7psia @14:08:08.83 CBV.dial SSV @14:08:09.16 SS.reconfigure :default @14:08:09.21 SS.seek 10ml @14:08:14.71 delay 15 seconds @14:08:29.82 Evacuated: Intake @17.8psia, Outlet @11.3psia @14:08:29.83 SSV.dial :spit,avoiding: :exhaust @14:08:31.15 SS.reconfigure :exhale @14:08:31.19 SS.seek 0ml @14:08:36.43 SS.reconfigure :fast @14:08:36.48 SS.seek 5ml @14:08:39.43 SSV.dial CBV,avoiding: :exhaust @14:08:39.45
CBV.dial SSV @14:08:40.79 CTV.dial :air,avoiding: :intake @14:08:42.14 CSV.dial CTV @14:08:42.18
SS.seek 10ml @14:08:43.17 CSV.dial :air @14:08:43.65 CS.reconfigure :fast @14:08:43.69 CS.seek 0ml @14:08:44.77
SSV.dial :spit,avoiding: :exhaust @14:08:46.09 SS.reconfigure :exhale @14:08:46.13 SS.seek 0ml @14:08:48.91 CS.stop @14:08:51.09
SS.reconfigure :fast @14:08:51.13 SS.seek 5ml @14:08:53.97 SSV.dial CBV,avoiding: :exhaust @14:08:53.99
CBV.dial SSV @14:08:56.31 SSV.dial :spit,avoiding: :exhaust @14:08:57.62 SS.reconfigure :default @14:08:57.66 SS.seek 0ml @14:09:00.84 SS.stop @14:09:00.88 Lysing: Adding 2.0ml AqMeOH @14:09:00.89 CBV.dial between CSV and waste1,avoiding: :DBV @14:09:02.41 CTV.dial CSV @14:09:03.74 CSV.dial CSR @14:09:04.52 Pulling 1ml of CSR.AqMeOH @14:09:04.53 CSR.select :AqMeOH @14:09:04.64 CS.reconfigure :default @14:09:04.68 CS.seek 1ml @14:09:08.07 delay 5 seconds @14:09:13.08 CSR.select :air @14:09:13.23 CS.seek 6ml @14:09:26.78 delay 2 seconds @14:09:28.80 CSV.dial :waste2 @14:09:29.23 CS.reconfigure :fast @14:09:29.27 CS.seek 0ml @14:09:43.78 CSV.dial CSR @14:09:44.29 Pulling 2ml of CSR.AqMeOH @14:09:44.30 CSR.select :AqMeOH @14:09:44.41 CS.reconfigure :default @14:09:44.45 CS.seek 2ml @14:09:50.44 delay 5 seconds @14:09:55.46 CSR.select :air @14:09:55.61 CS.seek 5ml @14:10:04.41 delay 2 seconds @14:10:06.42 CSV.dial CTV @14:10:06.85 CS.seek 1ml @14:10:17.98 Before lysing Collection Puck Heater at 12.9C/38.3psia @14:10:18.00 Heating Puck.from 2, :sh1 in Collection Clamp to 70C for 10:00 @14:10:18.01 CH.seek 70C,8:00 @14:12:22.06 CH.hold 10:00,70C @14:12:22.23 Begun lysing Collection Puck Heater at 68.1C/47.0psia @14:22:22.25 Ended lysing Collection Puck Heater at 70C/46.0psia @14:22:22.27 CS.seek 0ml @14:22:25.72 CTV.dial between spare and air,avoiding: :intake @14:22:26.76 CSV.dial :air @14:22:27.21 CS.seek 5ml @14:22:40.83 CSV.dial CTV @14:22:41.27 delay 2 seconds @14:22:43.29 CSV.dial :air @14:22:43.74 delay 2 seconds @14:22:45.76 CSV.dial CTV @14:22:46.22 delay 2 seconds @14:22:48.23 CSV.dial :air @14:22:48.68 delay 2 seconds @14:22:50.70 CS.seek 0ml @14:23:04.38 Puck @75.8psia after final lysis pressurization @14:23:04.40 Recovering Lysate into Collection syringe CSV.dial CBV @14:23:05.41 CBV.dial CSV @14:23:05.77 CS.seek 5ml @14:23:19.74 delay 5 seconds @14:23:24.76 CTV.dial :air @14:23:25.28 CS.seek 7ml @14:23:31.18 delay 5 seconds @14:23:36.19 CSV.dial :air2 @14:23:36.64 CS.seek 10ml @14:23:45.12 CSV.dial between air and CTV,avoiding: CBV @14:23:46.28 Lysing Complete @14:23:46.34 Storing sh1 puck in Collection Clamp to tube 1 @14:23:46.50 SC.reconfigure :tight @14:23:46.55 SC.seek 1 @14:23:50.09 SC.configure :limp @14:23:50.29 Elbow.reconfigure :tight @14:23:50.33 Elbow.seek 1 @14:23:52.10 Forearm.reconfigure :tight @14:23:52.14 Forearm.seek 1 + 450 counts @14:23:53.16 SE.reconfigure :pick @14:23:53.21 SE.seek :beamBreak @14:23:54.53 SE.configure :default @14:23:54.67 Elbow.reconfigure :limp @14:23:54.72 Forearm.reconfigure :loose @14:23:54.76 Forearm.seek 1 @14:23:55.53 Elbow.hold @14:23:55.59 Elbow.reconfigure :tight @14:23:55.69 SE.seek 27.07 Pucks below Top Plate @14:23:56.15 Forearm.force -20 @14:23:56.19 Forearm.seek 1 + 279 counts @14:23:56.47 Forearm.reconfigure :tight @14:23:56.51 Forearm.seek 1 + 450 counts @14:23:56.84 SE.seek 28.07 Pucks below Top Plate @14:23:57.40 Forearm.reconfigure :loose @14:23:57.44 Forearm.seek :retract @14:23:58.40 Elbow.seek CC @14:24:00.12 Elbow.reconfigure :loose @14:24:00.21 Forearm.seek CC @14:24:01.19 Hand.close @14:24:01.80 CC.seek :open @14:24:06.52 Forearm.seek :retract @14:24:07.51 Elbow.reconfigure :tight @14:24:07.55 Elbow.seek 1 @14:24:09.31 Elbow.reconfigure :limp @14:24:09.36 Forearm.seek 1 @14:24:10.33 Elbow.hold @14:24:10.39 Elbow.reconfigure :tight @14:24:10.43 Hand.open @14:24:11.16 Forearm.force -20 @14:24:11.20 Forearm.seek 1 + 317 counts @14:24:11.49 Forearm.reconfigure :tight @14:24:11.53 Forearm.seek 1 + 450 counts @14:24:11.77 SE.seek :down @14:24:12.62 SE.stop @14:24:12.90
Forearm.reconfigure :loose @14:24:12.94 Forearm.seek :retract @14:24:13.88 Tranferring DA @14:24:13.89 Beginning samplePassC @14:24:13.90 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:24:14.09 Elbow.seek FlushPuck::Garage @14:24:15.98 Elbow.reconfigure :loose @14:24:16.07 Forearm.seek :garage @14:24:17.02 Hand.close @14:24:17.79 Forearm.seek :retract @14:24:18.76 Elbow.reconfigure :tight @14:24:18.80 Elbow.seek CC @14:24:21.84 Elbow.reconfigure :loose @14:24:21.93 Forearm.reconfigure :tight @14:24:21.98 Forearm.seek CC @14:24:23.24 CC.seek :closed @14:24:27.75 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.37 @14:24:27.83 Hand.open @14:24:28.48 Forearm.reconfigure :loose @14:24:28.53 Forearm.seek :retract @14:24:29.47 PSV.dial PSR @14:24:30.86 Pulling 1.5ml of PSR.flush @14:24:30.87 PSR.select :flush @14:24:30.99 PS.seek 1.5ml @14:24:47.15 delay 5 seconds @14:24:52.17 PSV.dial :waste1 @14:24:52.64 PS.seek 0ml @14:25:08.84 PSV.dial PSR @14:25:09.42 PSR.select :air @14:25:09.53 PS.seek 2.5ml @14:25:35.97 PRV.dial CBV,avoiding: :ab2 @14:25:35.99
CBV.dial PRV @14:25:37.70 PSV.dial :mixloop @14:25:38.76 CTV.dial :air,avoiding: :intake @14:25:39.25 PTV.dial :PRVmixing @14:25:40.58 PS.seek 0ml @14:26:06.96 PSV.dial :air @14:26:07.42 PS.seek 2.5ml @14:26:33.71 PSV.dial :mixloop @14:26:34.19 PS.seek 0ml @14:27:00.59 PSV.dial :air @14:27:01.06 PS.seek 2.5ml @14:27:27.43 PSV.dial :mixloop @14:27:27.89 PS.seek 0ml @14:27:54.28 Garaging FlushPuck in Collection Clamp @14:27:54.34 Elbow.reconfigure :tight @14:27:54.39 Elbow.seek CC @14:27:54.79 Elbow.reconfigure :loose @14:27:54.88 Forearm.seek CC @14:27:55.86 Hand.close @14:27:56.43 CC.seek :open @14:28:01.03 Forearm.seek :retract @14:28:02.00 Elbow.reconfigure :tight @14:28:02.05 Elbow.seek :garage @14:28:05.06 Elbow.reconfigure :loose @14:28:05.14 Forearm.reconfigure :tight @14:28:05.19 Forearm.seek :garage @14:28:06.20 Hand.open @14:28:06.86 Forearm.reconfigure :loose @14:28:06.90 Forearm.seek :retract @14:28:07.88 PSV.dial :mixloop @14:28:07.89 PTV.dial :PRVmixing @14:28:07.90 PRV.dial CBV @14:28:07.93
CBV.dial PRV @14:28:07.94 CTV.dial :air,avoiding: :intake @14:28:07.96 Loading lyfil puck from tube 2 into Collection Clamp @14:28:08.18 SC.reconfigure :tight @14:28:08.22 SC.seek 2 @14:28:11.78 SC.configure :limp @14:28:11.97 Elbow.reconfigure :tight @14:28:12.01 Elbow.seek 2 @14:28:14.02 Forearm.reconfigure :tight @14:28:14.06 Forearm.seek 2 + 450 counts @14:28:15.12 SE.reconfigure :pick @14:28:15.17 SE.seek :beamBreak @14:28:18.12 SE.configure :default @14:28:18.26 Elbow.seek 2 @14:28:18.43 Forearm.seek 2 + 300 counts @14:28:18.59 Forearm.reconfigure :loose @14:28:18.64 Hand.close @14:28:19.31 SE.seek :down @14:28:19.49
Forearm.seek :retract @14:28:20.41 Elbow.seek CC @14:28:21.87 SE.stop @14:28:22.15
Elbow.reconfigure :loose @14:28:22.24 Forearm.reconfigure :tight @14:28:22.28 Forearm.seek CC @14:28:23.56 CC.seek :closed @14:28:28.06 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.11 @14:28:28.07 Hand.open @14:28:28.73 Forearm.reconfigure :loose @14:28:28.77 Forearm.seek :retract @14:28:29.74 CTV.dial CSV,avoiding: :intake @14:28:31.09 CSV.dial CTV @14:28:31.40 PS.seek 2.5ml @14:28:57.74 CS.seek 4.5ml @14:29:12.61 delay 8 seconds @14:29:20.63 PSV.dial :air @14:29:21.57 delay 2 seconds @14:29:23.58 PSV.dial :waste2 @14:29:24.34 PS.seek 0ml @14:29:50.77 CBV.dial between PRV and waste1 @14:29:51.10 CSV.dial :air @14:29:51.55 CS.seek 10ml @14:30:06.51 CSV.dial CTV @14:30:06.97 CS.seek 0ml @14:30:33.33 CBV.dial :waste1 @14:30:33.64 delay 5 seconds @14:30:38.66 CTV.dial :air,avoiding: :intake @14:30:40.06 Storing lyfil puck in Collection Clamp to tube 1 @14:30:40.22 SC.reconfigure :tight @14:30:40.26 SC.seek 1 @14:30:43.86 SC.configure :limp @14:30:44.06 Elbow.reconfigure :tight @14:30:44.10 Elbow.seek 1 @14:30:45.83 Forearm.reconfigure :tight @14:30:45.88 Forearm.seek 1 + 450 counts @14:30:46.90 SE.reconfigure :pick @14:30:46.95 SE.seek :beamBreak @14:30:48.18 SE.configure :default @14:30:48.33 Elbow.reconfigure :limp @14:30:48.37 Forearm.reconfigure :loose @14:30:48.42 Forearm.seek 1 @14:30:48.67 Elbow.hold @14:30:48.73 Elbow.reconfigure :tight @14:30:48.82 SE.seek 28.06 Pucks below Top Plate @14:30:49.29 Forearm.force -20 @14:30:49.32 Forearm.seek 1 + 306 counts @14:30:49.60 Forearm.reconfigure :tight @14:30:49.64 Forearm.seek 1 + 450 counts @14:30:49.84 SE.seek 29.06 Pucks below Top Plate @14:30:50.44 Forearm.reconfigure :loose @14:30:50.49 Forearm.seek :retract @14:30:51.43 Elbow.seek CC @14:30:53.13 Elbow.reconfigure :loose @14:30:53.22 Forearm.seek CC @14:30:54.21 Hand.close @14:30:54.80 CC.seek :open @14:30:59.52 Forearm.seek :retract @14:31:00.55 Elbow.reconfigure :tight @14:31:00.59 Elbow.seek 1 @14:31:02.37 Elbow.reconfigure :limp @14:31:02.42 Forearm.seek 1 @14:31:03.36 Elbow.hold @14:31:03.43 Elbow.reconfigure :tight @14:31:03.47 Hand.open @14:31:04.16 Forearm.force -20 @14:31:04.20 Forearm.seek 1 + 311 counts @14:31:04.48 Forearm.reconfigure :tight @14:31:04.52 Forearm.seek 1 + 450 counts @14:31:04.77 SE.seek :down @14:31:05.49 SE.stop @14:31:05.89
Forearm.reconfigure :loose @14:31:05.93 Forearm.seek :retract @14:31:06.86 PSV.dial :air @14:31:07.62 PS.seek 2.5ml @14:31:33.93 delay 1 second @14:31:34.94 PSV.dial :mixloop @14:31:35.38 delay 1 second @14:31:36.39 PSV.dial :air @14:31:36.85 delay 1 second @14:31:37.86 PSV.dial :mixloop @14:31:38.33 delay 1 second @14:31:39.35 PSV.dial :air @14:31:39.80 delay 1 second @14:31:40.82 PS.seek 0ml @14:32:07.21 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @14:32:07.85 Elbow.seek FlushPuck::Garage @14:32:09.65 Elbow.reconfigure :loose @14:32:09.74 Forearm.seek :garage @14:32:10.72 Hand.close @14:32:11.41 Forearm.seek :retract @14:32:12.38 Elbow.reconfigure :tight @14:32:12.43 Elbow.seek PC @14:32:13.68 Elbow.reconfigure :loose @14:32:13.77 Forearm.reconfigure :tight @14:32:13.81 Forearm.seek PC @14:32:15.02 PC.seek :closed @14:32:19.65 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.57 @14:32:19.71 Hand.open @14:32:20.35 Forearm.reconfigure :loose @14:32:20.40 Forearm.seek :retract @14:32:21.34 PRV.dial :air @14:32:21.80 PSV.dial :mixloop @14:32:22.33 PS.seek 0.3ml @14:32:26.17 PSV.dial PSR @14:32:27.28 Pulling 0.4ml of PSR.air PSR.select :air @14:32:27.31 PS.seek 0.7ml @14:32:32.14 delay 3 seconds @14:32:35.16 PSR.select :air @14:32:35.18 PS.seek 2.5ml @14:32:54.39 delay 3 seconds @14:32:57.41 PSV.dial :mixloop @14:32:58.46 PTV.dial :mixing @14:32:59.19 PBV.dial :waste2 @14:33:00.13 PS.seek 0ml @14:33:26.49 Garaging FlushPuck in Processing Clamp @14:33:26.56 Elbow.reconfigure :tight @14:33:26.60 Elbow.seek PC @14:33:26.70 Elbow.reconfigure :loose @14:33:26.79 Forearm.seek PC @14:33:27.75 Hand.close @14:33:28.30 PC.seek :open @14:33:33.00 Forearm.seek :retract @14:33:33.97 Elbow.reconfigure :tight @14:33:34.01 Elbow.seek :garage @14:33:35.33 Elbow.reconfigure :loose @14:33:35.42 Forearm.reconfigure :tight @14:33:35.47 Forearm.seek :garage @14:33:36.45 Hand.open @14:33:37.10 Forearm.reconfigure :loose @14:33:37.15 Forearm.seek :retract @14:33:38.10 Loading da puck from tube 2 into Processing Clamp @14:33:38.32 SC.reconfigure :tight @14:33:38.36 SC.seek 2 @14:33:41.91 SC.configure :limp @14:33:42.10 Elbow.reconfigure :tight @14:33:42.15 Elbow.seek 2 @14:33:44.03 Forearm.reconfigure :tight @14:33:44.07 Forearm.seek 2 + 450 counts @14:33:45.07 SE.reconfigure :pick @14:33:45.12 SE.seek :beamBreak @14:33:48.12 SE.configure :default @14:33:48.26 Elbow.seek 2 @14:33:48.42 Forearm.seek 2 + 300 counts @14:33:48.61 Forearm.reconfigure :loose @14:33:48.65 Hand.close @14:33:49.31 SE.seek :down @14:33:49.50
Forearm.seek :retract @14:33:50.45 Elbow.seek PC @14:33:51.97 SE.stop @14:33:53.08
Elbow.reconfigure :loose @14:33:53.65 Forearm.reconfigure :tight @14:33:53.70 Forearm.seek PC @14:33:54.96 PC.seek :closed @14:33:59.58 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.60 @14:33:59.59 Hand.open @14:34:00.23 Forearm.reconfigure :loose @14:34:00.27 Forearm.seek :retract @14:34:01.24 Heating Puck.from 2, :da in Processing Clamp to 28C for 2:00:00 @14:34:01.26 PH.seek 28C,5:00 @14:34:43.24 PH.hold 2:00:00,28C @14:34:43.28 delay 1:30 @14:36:13.32 PSR.select :air @14:36:13.34 PSV.dial PSR @14:36:14.45 Pulling 0.5ml of PSR.wash PSR.select :wash @14:36:14.57 PS.seek 0.5ml @14:36:20.52 delay 5 seconds @14:36:25.54 PSR.select :air @14:36:25.70 PS.seek 2ml @14:36:41.79 PTV.dial :puck @14:36:42.53 PSV.dial PTV @14:36:43.30 PBV.dial :waste1 @14:36:44.72 PS.seek 1.5ml @14:36:50.61 delay 3 seconds @14:36:53.62 PBV.dial between PSV and waste1 @14:36:53.99 PS.seek 0ml @14:37:10.14 delay 5:00 @14:42:10.16 recoverPSRreagent08 PTV.dial :puck @14:42:10.17 PBV.dial :waste1 @14:42:10.54 delay 30 seconds @14:42:40.57 PBV.dial between PSV and waste1 @14:42:40.93 PSR.select :air @14:42:40.95 PSV.dial PSR @14:42:41.72 PS.reconfigure :fast @14:42:41.81 PS.seek 2.5ml @14:43:05.54 PSV.dial PTV @14:43:06.29 delay 5 seconds @14:43:11.31 PSV.dial PSR @14:43:12.07 delay 5 seconds @14:43:17.09 PSV.dial PTV @14:43:17.85 delay 5 seconds @14:43:22.87 PSV.dial :air @14:43:23.64 PS.seek 0ml @14:43:46.54 PTV.dial :PRVmixing,via: PRV @14:43:47.87 PSV.dial :mixloop @14:43:48.34 PRV.dial :ab1 @14:43:49.11 PS.reconfigure :slow @14:43:49.15 PS.seek 0.15ml @14:43:52.76 delay 2 seconds @14:43:54.78 CBV.dial PRV @14:43:54.83
PRV.dial CBV @14:43:55.35 PS.seek 0.35ml @14:44:00.04 delay 2 seconds @14:44:02.05 CBV.dial between PRV and PSV @14:44:02.36 PRV.dial :air @14:44:02.81 PS.seek 0.9ml @14:44:14.63 PRV.dial :waste2 @14:44:15.39 PS.seek 0.5ml @14:44:24.01 addPMLReagent08 @14:44:24.02 PSV.dial :air @14:44:24.48 PS.reconfigure :default @14:44:24.53 PS.seek 2ml @14:44:40.63 PSV.dial :PML @14:44:41.08 PTV.dial :mixing @14:44:41.82 PBV.dial :waste1 @14:44:42.25 PS.seek 1.85ml @14:44:44.69 PBV.dial between PSV and waste1 @14:44:45.55 PS.seek 0ml @14:45:05.22 delay 2 seconds @14:45:07.24 Beginning samplePassC @14:45:07.26 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:45:07.42 Elbow.reconfigure :tight @14:45:07.47 Elbow.seek FlushPuck::Garage @14:45:08.83 Elbow.reconfigure :loose @14:45:08.92 Forearm.seek :garage @14:45:09.96 Hand.close @14:45:10.66 Forearm.seek :retract @14:45:11.63 Elbow.reconfigure :tight @14:45:11.68 Elbow.seek CC @14:45:14.76 Elbow.reconfigure :loose @14:45:14.85 Forearm.reconfigure :tight @14:45:14.89 Forearm.seek CC @14:45:16.08 CC.seek :closed @14:45:20.60 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.37 @14:45:20.67 Hand.open @14:45:21.35 Forearm.reconfigure :loose @14:45:21.39 Forearm.seek :retract @14:45:22.39 PSV.dial PSR @14:45:23.50 Pulling 1.5ml of PSR.flush @14:45:23.51 PSR.select :flush @14:45:23.63 PS.seek 1.5ml @14:45:39.79 delay 5 seconds @14:45:44.81 PSV.dial :waste1 @14:45:45.26 PS.seek 0ml @14:46:01.41 PSV.dial PSR @14:46:01.86 PSR.select :air @14:46:01.98 PS.seek 2.5ml @14:46:28.39 PRV.dial CBV,avoiding: :ab2 @14:46:28.40
CBV.dial PRV @14:46:30.04 PSV.dial :mixloop @14:46:31.08 CTV.dial :air,avoiding: :intake @14:46:31.10 PTV.dial :PRVmixing @14:46:31.87 PS.seek 0ml @14:46:58.32 PSV.dial :air @14:46:58.78 PS.seek 2.5ml @14:47:25.10 PSV.dial :mixloop @14:47:25.57 PS.seek 0ml @14:47:52.10 PSV.dial :air @14:47:52.56 PS.seek 2.5ml @14:48:18.94 PSV.dial :mixloop @14:48:19.40 PS.seek 0ml @14:48:45.72 Garaging FlushPuck in Collection Clamp @14:48:45.79 Elbow.reconfigure :tight @14:48:45.83 Elbow.seek CC @14:48:46.03 Elbow.reconfigure :loose @14:48:46.11 Forearm.seek CC @14:48:47.06 Hand.close @14:48:47.67 CC.seek :open @14:48:52.27 Forearm.seek :retract @14:48:53.21 Elbow.reconfigure :tight @14:48:53.25 Elbow.seek :garage @14:48:56.17 Elbow.reconfigure :loose @14:48:56.25 Forearm.reconfigure :tight @14:48:56.30 Forearm.seek :garage @14:48:57.32 Hand.open @14:48:57.97 Forearm.reconfigure :loose @14:48:58.01 Forearm.seek :retract @14:48:58.97 delayUntil "14:53:07.23" @14:48:59.00 Whole Cell Archive sampling 1000ml @14:48:59.02 Loading wcr puck from tube 2 into Collection Clamp @14:48:59.29 SC.reconfigure :tight @14:48:59.34 SC.seek 2 @14:48:59.47 SC.configure :limp @14:49:00.15 Elbow.reconfigure :tight @14:49:00.19 Elbow.seek 2 @14:49:02.14 Forearm.reconfigure :tight @14:49:02.19 Forearm.seek 2 + 450 counts @14:49:03.22 SE.reconfigure :pick @14:49:03.28 SE.seek :beamBreak @14:49:06.32 SE.configure :default @14:49:06.47 Elbow.seek 2 @14:49:06.65 Forearm.seek 2 + 300 counts @14:49:06.89 Forearm.reconfigure :loose @14:49:06.94 Hand.close @14:49:07.55 SE.seek :down @14:49:07.76 Forearm.seek :retract @14:49:08.70 Elbow.seek CC @14:49:10.26 SE.stop @14:49:10.44 Elbow.reconfigure :loose @14:49:10.53 Forearm.reconfigure :tight @14:49:10.58 Forearm.seek CC @14:49:11.83 CC.seek :closed @14:49:16.35 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.25 @14:49:16.36 Hand.open @14:49:16.97 Forearm.reconfigure :loose @14:49:17.02 Forearm.seek :retract @14:49:18.01 collectSample 1000ml w/bubble point of 35psi @14:49:18.03 Priming 7ml @14:49:18.04 Intake.select :close @14:49:18.05 Exhaust.select :close @14:49:18.06 SS.hold @14:49:18.25 Calibrating pressure sensors @14:49:18.26 CTV.dial :air @14:49:18.27 SSV.dial :spit,avoiding: :exhaust @14:49:18.29 SS.reconfigure :fast @14:49:18.34 SS.seek 5ml @14:49:21.16 SSV.dial CBV @14:49:21.18 CBV.dial SSV @14:49:22.54 delay 3.8 seconds @14:49:26.46 SSV.dial :spit @14:49:27.80 SS.seek 0ml @14:49:30.37 SSV.dial CBV @14:49:31.68 CTV.dial :intake @14:49:32.45 SSV.dial CBV @14:49:32.48 CBV.dial SSV @14:49:32.49 Allowing 40s for 7.0ml of remaining 7.0ml @14:49:32.50 Intake.select :open @14:49:34.66 Intake @15.3psia, vacuum from 9.8 to 12.8psi @14:49:34.68 SS.reconfigure :inhaling 2.20ml/s @14:49:34.73 SS.seek 7ml @14:49:40.99 Stroke completed -- waiting for pressure to equalize ... @14:49:41.00 SS.reconfigure :exhale @14:49:41.04 SS.hold @14:49:50.72 Intake.select :close @14:49:50.87 SSV.dial :exhaust @14:49:51.64 Exhaust.select :open @14:49:51.75 SS.seek 0ml @14:49:56.27 Exhaust.select :close @14:49:56.38 Sampling 1000.0ml @14:49:56.39 Intake.select :close @14:49:56.40 Exhaust.select :close @14:49:56.41 SS.reconfigure :default @14:49:56.45 SS.hold @14:49:56.65 CTV.dial :intake @14:49:56.67 SSV.dial CBV @14:49:56.69 CBV.dial SSV @14:49:57.48 Allowing 130s for 25.0ml of remaining 1000.0ml @14:49:57.49 Intake.select :open @14:49:59.64 Intake @16.1psia, vacuum from 10.0 to 13.0psi @14:49:59.66 SS.reconfigure :inhaling 2.20ml/s @14:49:59.71 SS.seek 25ml @14:50:05.29 Vacuum too high -- waiting up to 40 seconds @14:50:05.31 SS.reconfigure :exhale @14:50:05.35 SS.hold @14:50:06.98 Intake @13.8psia, vacuum from 8.3 to 11.3psi @14:50:07.00 SS.reconfigure :inhaling 1.98ml/s @14:50:07.04 SS.seek 25ml @14:50:13.89 Vacuum too high -- waiting up to 40 seconds @14:50:13.90 SS.reconfigure :exhale @14:50:13.94 SS.hold @14:50:19.85 Intake @14.0psia, vacuum from 8.5 to 11.5psi @14:50:19.87 SS.reconfigure :inhaling 1.79ml/s @14:50:19.91 SS.seek 25ml @14:50:24.26 Stroke completed -- waiting for pressure to equalize ... @14:50:24.27 SS.reconfigure :exhale @14:50:24.31 SS.hold @14:50:28.41 Intake.select :close @14:50:28.55 SSV.dial :exhaust @14:50:29.34 Exhaust.select :open @14:50:29.45 SS.seek 0ml @14:50:38.21 Exhaust.select :close @14:50:38.31 SSV.dial CBV @14:50:38.34 CBV.dial SSV @14:50:39.10 Allowing 130s for 25.0ml of remaining 975.0ml @14:50:39.11 Intake.select :open @14:50:41.27 Intake @15.7psia, vacuum from 10.0 to 13.0psi @14:50:41.29 SS.reconfigure :inhaling 1.79ml/s @14:50:41.33 SS.seek 25ml @14:50:52.98 Vacuum too high -- waiting up to 40 seconds @14:50:52.99 SS.reconfigure :exhale @14:50:53.04 SS.hold @14:50:53.64 Intake @12.5psia, vacuum from 7.0 to 10.0psi @14:50:53.65 SS.reconfigure :inhaling 1.61ml/s @14:50:53.70 SS.seek 25ml @14:51:00.35 Stroke completed -- waiting for pressure to equalize ... @14:51:00.36 SS.reconfigure :exhale @14:51:00.40 SS.hold @14:51:07.80 Intake.select :close @14:51:07.94 SSV.dial :exhaust @14:51:08.71 Exhaust.select :open @14:51:08.82 SS.seek 0ml @14:51:17.57 Exhaust.select :close @14:51:17.67 SSV.dial CBV @14:51:17.70 CBV.dial SSV @14:51:18.46 Allowing 130s for 25.0ml of remaining 950.0ml @14:51:18.47 Intake.select :open @14:51:20.63 Intake @15.5psia, vacuum from 10.0 to 13.0psi @14:51:20.65 SS.reconfigure :inhaling 1.61ml/s @14:51:20.69 SS.seek 25ml @14:51:37.62 Vacuum too high -- waiting up to 40 seconds @14:51:37.63 SS.reconfigure :exhale @14:51:37.68 SS.hold @14:51:38.19 Intake @12.3psia, vacuum from 6.8 to 9.8psi @14:51:38.21 SS.reconfigure :inhaling 1.45ml/s @14:51:38.26 SS.seek 25ml @14:51:40.69 Stroke completed -- waiting for pressure to equalize ... @14:51:40.70 SS.reconfigure :exhale @14:51:40.75 SS.hold @14:51:48.15 Intake.select :close @14:51:48.29 SSV.dial :exhaust @14:51:49.07 Exhaust.select :open @14:51:49.18 SS.seek 0ml @14:51:58.11 Exhaust.select :close @14:51:58.22 SSV.dial CBV @14:51:58.24 CBV.dial SSV @14:51:59.04 Allowing 130s for 25.0ml of remaining 925.0ml @14:51:59.05 Intake.select :open @14:52:01.20 Intake @15.5psia, vacuum from 10.0 to 13.0psi @14:52:01.22 SS.reconfigure :inhaling 1.45ml/s @14:52:01.27 SS.seek 25ml @14:52:20.99 Stroke completed -- waiting for pressure to equalize ... @14:52:21.00 SS.reconfigure :exhale @14:52:21.04 SS.hold @14:52:29.51 Intake.select :close @14:52:29.65 SSV.dial :exhaust @14:52:30.42 Exhaust.select :open @14:52:30.54 SS.seek 0ml @14:52:39.31 Exhaust.select :close @14:52:39.42 SSV.dial CBV @14:52:39.44 CBV.dial SSV @14:52:40.22 Allowing 130s for 25.0ml of remaining 900.0ml @14:52:40.24 Intake.select :open @14:52:42.39 Intake @15.4psia, vacuum from 9.9 to 12.9psi @14:52:42.41 SS.reconfigure :inhaling 1.45ml/s @14:52:42.45 SS.seek 25ml @14:53:00.01 Vacuum too high -- waiting up to 40 seconds @14:53:00.02 SS.reconfigure :exhale @14:53:00.07 SS.hold @14:53:01.16 Intake @12.6psia, vacuum from 7.1 to 10.1psi @14:53:01.18 SS.reconfigure :inhaling 1.30ml/s @14:53:01.22 SS.seek 25ml @14:53:04.61 Stroke completed -- waiting for pressure to equalize ... @14:53:04.62 SS.reconfigure :exhale @14:53:04.67 SS.hold @14:53:07.24 recoverPRVreagent08 @14:53:07.25 PTV.dial :mixing @14:53:08.05 PBV.dial :waste1 @14:53:08.47 delay 30 seconds @14:53:12.08 Intake.select :close @14:53:12.22 SSV.dial :exhaust @14:53:12.99 Exhaust.select :open @14:53:13.10 SS.seek 0ml @14:53:21.91 Exhaust.select :close @14:53:22.01 SSV.dial CBV @14:53:22.03 CBV.dial SSV @14:53:22.80 Allowing 130s for 25.0ml of remaining 875.0ml @14:53:23.30 Intake.select :open @14:53:25.45 Intake @15.5psia, vacuum from 10.0 to 13.0psi @14:53:25.47 SS.reconfigure :inhaling 1.30ml/s @14:53:25.52 SS.seek 25ml @14:53:38.49 PBV.dial between PSV and waste1 @14:53:38.88 PSV.dial :air @14:53:39.49 PS.reconfigure :fast @14:53:39.53 PS.seek 2.5ml @14:53:46.70 Vacuum too high -- waiting up to 40 seconds @14:53:46.71 SS.reconfigure :exhale @14:53:46.75 SS.hold @14:53:48.92 Intake @12.9psia, vacuum from 7.4 to 10.4psi @14:53:48.94 SS.reconfigure :inhaling 1.17ml/s @14:53:48.98 SS.seek 25ml @14:53:50.23 Stroke completed -- waiting for pressure to equalize ... @14:53:50.25 SS.reconfigure :exhale @14:53:50.29 SS.hold @14:53:56.76 Intake.select :close @14:53:56.90 SSV.dial :exhaust @14:53:57.67 Exhaust.select :open @14:53:57.79 SS.seek 0ml @14:54:03.46 PSV.dial :mixloop @14:54:03.92 delay 2 seconds @14:54:05.94 PSV.dial :air @14:54:06.53 delay 2 seconds @14:54:06.75 Exhaust.select :close @14:54:06.86 SSV.dial CBV @14:54:06.88 CBV.dial SSV @14:54:07.66 Allowing 130s for 25.0ml of remaining 850.0ml @14:54:07.67 Intake.select :open @14:54:08.55 PSV.dial :mixloop @14:54:09.00 delay 2 seconds @14:54:09.83 Intake @15.4psia, vacuum from 9.9 to 12.9psi @14:54:09.85 SS.reconfigure :inhaling 1.17ml/s @14:54:09.90 SS.seek 25ml @14:54:11.02 PSV.dial :air @14:54:11.63 PS.seek 0ml @14:54:33.50 Stroke completed -- waiting for pressure to equalize ... @14:54:33.51 SS.reconfigure :exhale @14:54:33.56 SS.hold @14:54:34.93 washCycleDA #1 of 2 @14:54:34.94 PSR.select :air @14:54:34.96 PSV.dial PSR @14:54:36.35 Pulling 0.5ml of PSR.wash @14:54:36.36 PSR.select :wash @14:54:36.53 PS.reconfigure :default @14:54:36.58 PS.seek 0.5ml @14:54:42.53 delay 5 seconds @14:54:43.34 Intake.select :close @14:54:43.48 SSV.dial :exhaust @14:54:44.26 Exhaust.select :open @14:54:44.37 SS.seek 0ml @14:54:47.54 PSR.select :air @14:54:47.65 PS.seek 2ml @14:54:53.06 Exhaust.select :close @14:54:53.17 SSV.dial CBV @14:54:53.19 CBV.dial SSV @14:54:53.98 Allowing 130s for 25.0ml of remaining 825.0ml @14:54:53.99 Intake.select :open @14:54:56.15 Intake @15.3psia, vacuum from 9.8 to 12.8psi @14:54:56.17 SS.reconfigure :inhaling 1.17ml/s @14:54:56.21 SS.seek 25ml @14:55:03.78 PTV.dial :puck @14:55:04.53 PSV.dial PTV @14:55:05.42 PBV.dial :waste1 @14:55:05.87 PS.seek 1.5ml @14:55:11.84 delay 3 seconds @14:55:14.86 PBV.dial between PSV and waste1 @14:55:15.74 PS.seek 0ml @14:55:19.54 Vacuum too high -- waiting up to 40 seconds @14:55:19.55 SS.reconfigure :exhale @14:55:19.60 SS.hold @14:55:23.75 Intake @13.2psia, vacuum from 7.7 to 10.7psi @14:55:23.77 SS.reconfigure :inhaling 1.06ml/s @14:55:23.81 SS.seek 25ml @14:55:25.04 Stroke completed -- waiting for pressure to equalize ... @14:55:25.05 SS.reconfigure :exhale @14:55:25.09 SS.hold @14:55:30.64 Intake.select :close @14:55:30.78 SSV.dial :exhaust @14:55:31.55 Exhaust.select :open @14:55:31.66 SS.seek 0ml @14:55:31.87 delay 2:00 @14:55:40.34 Exhaust.select :close @14:55:40.45 SSV.dial CBV @14:55:40.47 CBV.dial SSV @14:55:41.75 Allowing 130s for 25.0ml of remaining 800.0ml @14:55:41.76 Intake.select :open @14:55:43.92 Intake @15.3psia, vacuum from 9.8 to 12.8psi @14:55:43.94 SS.reconfigure :inhaling 1.06ml/s @14:55:43.98 SS.seek 25ml @14:56:10.04 Stroke completed -- waiting for pressure to equalize ... @14:56:10.06 SS.reconfigure :exhale @14:56:10.10 SS.hold @14:56:19.80 Intake.select :close @14:56:19.94 SSV.dial :exhaust @14:56:20.71 Exhaust.select :open @14:56:20.82 SS.seek 0ml @14:56:29.59 Exhaust.select :close @14:56:29.70 SSV.dial CBV @14:56:29.72 CBV.dial SSV @14:56:30.50 Allowing 130s for 25.0ml of remaining 775.0ml @14:56:30.51 Intake.select :open @14:56:32.67 Intake @15.4psia, vacuum from 9.9 to 12.9psi @14:56:32.69 SS.reconfigure :inhaling 1.06ml/s @14:56:32.73 SS.seek 25ml @14:56:56.76 Vacuum too high -- waiting up to 40 seconds @14:56:56.77 SS.reconfigure :exhale @14:56:56.81 SS.hold @14:57:00.98 Intake @13.3psia, vacuum from 7.8 to 10.8psi @14:57:01.00 SS.reconfigure :inhaling 0.95ml/s @14:57:01.05 SS.seek 25ml @14:57:04.11 Stroke completed -- waiting for pressure to equalize ... @14:57:04.12 SS.reconfigure :exhale @14:57:04.16 SS.hold @14:57:10.21 Intake.select :close @14:57:10.35 SSV.dial :exhaust @14:57:11.12 Exhaust.select :open @14:57:11.24 SS.seek 0ml @14:57:20.01 Exhaust.select :close @14:57:20.15 SSV.dial CBV @14:57:20.18 CBV.dial SSV @14:57:20.95 Allowing 130s for 25.0ml of remaining 750.0ml @14:57:20.97 Intake.select :open @14:57:23.12 Intake @15.3psia, vacuum from 9.8 to 12.8psi @14:57:23.14 SS.reconfigure :inhaling 0.95ml/s @14:57:23.19 SS.seek 25ml @14:57:31.89 recoverPSRreagent08 @14:57:31.91 PTV.dial :puck @14:57:31.92 PBV.dial :waste1 @14:57:32.31 delay 30 seconds @14:57:52.23 Stroke completed -- waiting for pressure to equalize ... @14:57:52.24 SS.reconfigure :exhale @14:57:52.29 SS.hold @14:58:02.32 PBV.dial between PSV and waste1 @14:58:02.71 PSR.select :air @14:58:02.72 PSV.dial PSR @14:58:03.33 Intake.select :close @14:58:03.49 SSV.dial :exhaust @14:58:03.52 PS.reconfigure :fast @14:58:03.61 PS.seek 2.5ml @14:58:04.31 Exhaust.select :open @14:58:04.42 SS.seek 0ml @14:58:13.40 Exhaust.select :close @14:58:13.51 SSV.dial CBV @14:58:13.53 CBV.dial SSV @14:58:14.31 Allowing 130s for 25.0ml of remaining 725.0ml @14:58:14.32 Intake.select :open @14:58:16.48 Intake @15.2psia, vacuum from 9.7 to 12.7psi @14:58:16.50 SS.reconfigure :inhaling 0.95ml/s @14:58:16.54 SS.seek 25ml @14:58:27.52 PSV.dial PTV @14:58:28.29 delay 5 seconds @14:58:33.31 PSV.dial PSR @14:58:34.07 delay 5 seconds @14:58:39.09 PSV.dial PTV @14:58:39.85 delay 5 seconds @14:58:42.57 Vacuum too high -- waiting up to 40 seconds @14:58:42.58 SS.reconfigure :exhale @14:58:42.62 SS.hold @14:58:44.87 PSV.dial :air @14:58:45.76 PS.seek 0ml @14:58:47.52 Intake @13.5psia, vacuum from 8.0 to 11.0psi @14:58:47.54 SS.reconfigure :inhaling 0.87ml/s @14:58:47.58 SS.seek 25ml @14:58:50.63 Stroke completed -- waiting for pressure to equalize ... @14:58:50.65 SS.reconfigure :exhale @14:58:50.69 SS.hold @14:58:57.10 Intake.select :close @14:58:57.24 SSV.dial :exhaust @14:58:58.00 Exhaust.select :open @14:58:58.11 SS.seek 0ml @14:59:07.06 Exhaust.select :close @14:59:07.17 SSV.dial CBV @14:59:07.20 CBV.dial SSV @14:59:07.95 Allowing 130s for 25.0ml of remaining 700.0ml @14:59:07.96 Intake.select :open @14:59:09.51 washCycleDA #2 of 2 @14:59:09.52 PSR.select :air @14:59:09.54 PSV.dial PSR @14:59:10.12 Intake @15.3psia, vacuum from 9.8 to 12.8psi @14:59:10.14 SS.reconfigure :inhaling 0.87ml/s @14:59:10.19 SS.seek 25ml @14:59:11.06 Pulling 0.5ml of PSR.wash @14:59:11.07 PSR.select :wash @14:59:11.18 PS.reconfigure :default @14:59:11.22 PS.seek 0.5ml @14:59:17.09 delay 5 seconds @14:59:22.11 PSR.select :air @14:59:22.22 PS.seek 2ml @14:59:38.30 PTV.dial :puck @14:59:38.32 PSV.dial PTV @14:59:39.06 PBV.dial :waste1 @14:59:39.49 PS.seek 1.5ml @14:59:41.12 Stroke completed -- waiting for pressure to equalize ... @14:59:41.13 SS.reconfigure :exhale @14:59:41.18 SS.hold @14:59:45.46 delay 3 seconds @14:59:48.54 PBV.dial between PSV and waste1 @14:59:48.92 PS.seek 0ml @14:59:53.80 Intake.select :close @14:59:53.94 SSV.dial :exhaust @14:59:54.71 Exhaust.select :open @14:59:54.82 SS.seek 0ml @15:00:03.85 Exhaust.select :close @15:00:03.96 SSV.dial CBV @15:00:03.98 CBV.dial SSV @15:00:04.76 Allowing 130s for 25.0ml of remaining 675.0ml @15:00:04.77 Intake.select :open @15:00:05.06 delay 2:00 @15:00:06.93 Intake @15.2psia, vacuum from 9.7 to 12.7psi @15:00:06.95 SS.reconfigure :inhaling 0.87ml/s @15:00:06.99 SS.seek 25ml @15:00:35.03 Vacuum too high -- waiting up to 40 seconds @15:00:35.05 SS.reconfigure :exhale @15:00:35.09 SS.hold @15:00:39.97 Intake @13.7psia, vacuum from 8.2 to 11.2psi @15:00:39.99 SS.reconfigure :inhaling 0.78ml/s @15:00:40.04 SS.seek 25ml @15:00:43.43 Stroke completed -- waiting for pressure to equalize ... @15:00:43.44 SS.reconfigure :exhale @15:00:43.49 SS.hold @15:00:51.96 Intake.select :close @15:00:52.10 SSV.dial :exhaust @15:00:52.87 Exhaust.select :open @15:00:52.98 SS.seek 0ml @15:01:01.76 Exhaust.select :close @15:01:01.87 SSV.dial CBV @15:01:01.90 CBV.dial SSV @15:01:02.65 Allowing 130s for 25.0ml of remaining 650.0ml @15:01:02.66 Intake.select :open @15:01:04.82 Intake @15.1psia, vacuum from 9.6 to 12.6psi @15:01:04.84 SS.reconfigure :inhaling 0.78ml/s @15:01:04.88 SS.seek 25ml @15:01:38.85 Vacuum too high -- waiting up to 40 seconds @15:01:38.86 SS.reconfigure :exhale @15:01:38.90 SS.hold @15:01:45.05 Intake @13.8psia, vacuum from 8.3 to 11.3psi @15:01:45.07 SS.reconfigure :inhaling 0.70ml/s @15:01:45.11 SS.seek 25ml @15:01:46.13 Stroke completed -- waiting for pressure to equalize ... @15:01:46.14 SS.reconfigure :exhale @15:01:46.19 SS.hold @15:01:52.61 Intake.select :close @15:01:52.75 SSV.dial :exhaust @15:01:53.51 Exhaust.select :open @15:01:53.62 SS.seek 0ml @15:02:02.40 Exhaust.select :close @15:02:02.50 SSV.dial CBV @15:02:02.52 CBV.dial SSV @15:02:03.29 Allowing 130s for 25.0ml of remaining 625.0ml @15:02:03.31 Intake.select :open @15:02:05.07 recoverPSRreagent08 @15:02:05.09 PTV.dial :puck @15:02:05.10 PBV.dial :waste1 @15:02:05.48 delay 30 seconds @15:02:05.50 Intake @15.2psia, vacuum from 9.7 to 12.7psi @15:02:05.51 SS.reconfigure :inhaling 0.70ml/s @15:02:05.56 SS.seek 25ml @15:02:35.51 PBV.dial between PSV and waste1 @15:02:35.88 PSR.select :air @15:02:35.90 PSV.dial PSR @15:02:36.67 PS.reconfigure :fast @15:02:36.71 PS.seek 2.5ml @15:02:43.47 Stroke completed -- waiting for pressure to equalize ... @15:02:43.49 SS.reconfigure :exhale @15:02:43.53 SS.hold @15:02:56.10 Intake.select :close @15:02:56.24 SSV.dial :exhaust @15:02:57.01 Exhaust.select :open @15:02:57.12 SS.seek 0ml @15:03:00.64 PSV.dial PTV @15:03:01.41 delay 5 seconds @15:03:05.89 Exhaust.select :close @15:03:05.99 SSV.dial CBV @15:03:06.03 CBV.dial SSV @15:03:06.43 PSV.dial PSR @15:03:06.77 Allowing 130s for 25.0ml of remaining 600.0ml @15:03:06.79 Intake.select :open @15:03:07.19 delay 5 seconds @15:03:08.94 Intake @15.1psia, vacuum from 9.6 to 12.6psi @15:03:08.96 SS.reconfigure :inhaling 0.70ml/s @15:03:09.00 SS.seek 25ml @15:03:12.21 PSV.dial PTV @15:03:12.99 delay 5 seconds @15:03:18.01 PSV.dial :air @15:03:18.78 PS.seek 0ml @15:03:41.96 getPMLReagent08 @15:03:41.97 PRV.dial :air @15:03:42.43 delay 5 seconds @15:03:43.31 Vacuum too high -- waiting up to 40 seconds @15:03:43.32 SS.reconfigure :exhale @15:03:43.37 SS.hold @15:03:47.44 PTV.dial :PRVmixing @15:03:48.77 delay 5 seconds @15:03:50.39 Intake @14.0psia, vacuum from 8.5 to 11.5psi @15:03:50.41 SS.reconfigure :inhaling 0.63ml/s @15:03:50.45 SS.seek 25ml @15:03:53.78 PRV.dial :ab2 @15:03:54.25 PTV.dial :PRVmixing @15:03:54.27 PSV.dial :mixloop @15:03:54.74 PS.reconfigure :default @15:03:54.79 PS.seek 0.3ml @15:03:55.04 Stroke completed -- waiting for pressure to equalize ... @15:03:55.05 SS.reconfigure :exhale @15:03:55.09 SS.hold @15:03:58.87 delay 2 seconds @15:04:00.88 PRV.dial :air @15:04:01.46 PS.seek 0.55ml @15:04:02.53 Intake.select :close @15:04:02.67 SSV.dial :exhaust @15:04:03.43 Exhaust.select :open @15:04:03.54 SS.seek 0ml @15:04:04.86 addPMLReagent08 @15:04:04.87 PSV.dial :air @15:04:05.34 PS.seek 2ml @15:04:12.35 Exhaust.select :close @15:04:12.46 SSV.dial CBV @15:04:12.48 CBV.dial SSV @15:04:13.26 Allowing 130s for 25.0ml of remaining 575.0ml @15:04:13.27 Intake.select :open @15:04:15.42 Intake @15.2psia, vacuum from 9.7 to 12.7psi @15:04:15.44 SS.reconfigure :inhaling 0.63ml/s @15:04:15.49 SS.seek 25ml @15:04:20.92 PSV.dial :PML @15:04:21.40 PTV.dial :mixing @15:04:22.16 PBV.dial :waste1 @15:04:22.58 PS.seek 1.85ml @15:04:25.02 PBV.dial between PSV and waste1 @15:04:25.40 PS.seek 0ml @15:04:45.11 delay 2 seconds @15:04:47.13 delay 8:00 @15:04:57.57 Stroke completed -- waiting for pressure to equalize ... @15:04:57.58 SS.reconfigure :exhale @15:04:57.62 SS.hold @15:05:11.92 Intake.select :close @15:05:12.06 SSV.dial :exhaust @15:05:12.82 Exhaust.select :open @15:05:12.93 SS.seek 0ml @15:05:21.67 Exhaust.select :close @15:05:21.78 SSV.dial CBV @15:05:21.80 CBV.dial SSV @15:05:22.58 Allowing 130s for 25.0ml of remaining 550.0ml @15:05:22.60 Intake.select :open @15:05:24.75 Intake @15.3psia, vacuum from 9.8 to 12.8psi @15:05:24.77 SS.reconfigure :inhaling 0.63ml/s @15:05:24.81 SS.seek 25ml @15:06:01.09 Vacuum too high -- waiting up to 40 seconds @15:06:01.10 SS.reconfigure :exhale @15:06:01.15 SS.hold @15:06:08.64 Intake @14.2psia, vacuum from 8.7 to 11.7psi @15:06:08.66 SS.reconfigure :inhaling 0.57ml/s @15:06:08.70 SS.seek 25ml @15:06:15.39 Stroke completed -- waiting for pressure to equalize ... @15:06:15.40 SS.reconfigure :exhale @15:06:15.45 SS.hold @15:06:25.13 Intake.select :close @15:06:25.27 SSV.dial :exhaust @15:06:26.06 Exhaust.select :open @15:06:26.17 SS.seek 0ml @15:06:34.86 Exhaust.select :close @15:06:34.97 SSV.dial CBV @15:06:34.99 CBV.dial SSV @15:06:35.77 Allowing 130s for 25.0ml of remaining 525.0ml @15:06:35.78 Intake.select :open @15:06:37.94 Intake @15.3psia, vacuum from 9.8 to 12.8psi @15:06:37.96 SS.reconfigure :inhaling 0.57ml/s @15:06:38.00 SS.seek 25ml @15:07:21.93 Vacuum too high -- waiting up to 40 seconds @15:07:21.94 SS.reconfigure :exhale @15:07:21.99 SS.hold @15:07:30.55 Intake @14.4psia, vacuum from 8.9 to 11.9psi @15:07:30.57 SS.reconfigure :inhaling 0.51ml/s @15:07:30.62 SS.seek 25ml @15:07:33.33 Stroke completed -- waiting for pressure to equalize ... @15:07:33.35 SS.reconfigure :exhale @15:07:33.39 SS.hold @15:07:41.86 Intake.select :close @15:07:42.00 SSV.dial :exhaust @15:07:42.77 Exhaust.select :open @15:07:42.88 SS.seek 0ml @15:07:51.69 Exhaust.select :close @15:07:51.80 SSV.dial CBV @15:07:51.83 CBV.dial SSV @15:07:52.58 Allowing 130s for 25.0ml of remaining 500.0ml @15:07:52.59 Intake.select :open @15:07:54.75 Intake @15.3psia, vacuum from 9.8 to 12.8psi @15:07:54.77 SS.reconfigure :inhaling 0.51ml/s @15:07:54.81 SS.seek 25ml @15:08:45.95 Stroke completed -- waiting for pressure to equalize ... @15:08:45.96 SS.reconfigure :exhale @15:08:46.01 SS.hold @15:09:04.26 Intake.select :close @15:09:04.40 SSV.dial :exhaust @15:09:05.16 Exhaust.select :open @15:09:05.27 SS.seek 0ml @15:09:14.08 Exhaust.select :close @15:09:14.19 SSV.dial CBV @15:09:14.22 CBV.dial SSV @15:09:14.97 Allowing 130s for 25.0ml of remaining 475.0ml @15:09:14.98 Intake.select :open @15:09:17.14 Intake @15.4psia, vacuum from 9.9 to 12.9psi @15:09:17.16 SS.reconfigure :inhaling 0.51ml/s @15:09:17.20 SS.seek 25ml @15:09:52.27 Vacuum too high -- waiting up to 40 seconds @15:09:52.29 SS.reconfigure :exhale @15:09:52.33 SS.hold @15:09:59.87 Intake @14.6psia, vacuum from 9.1 to 12.1psi @15:09:59.89 SS.reconfigure :inhaling 0.47ml/s @15:09:59.93 SS.seek 25ml @15:10:17.62 Stroke completed -- waiting for pressure to equalize ... @15:10:17.63 SS.reconfigure :exhale @15:10:17.68 SS.hold @15:10:31.96 Intake.select :close @15:10:32.10 SSV.dial :exhaust @15:10:32.86 Exhaust.select :open @15:10:32.97 SS.seek 0ml @15:10:41.69 Exhaust.select :close @15:10:41.79 SSV.dial CBV @15:10:41.81 CBV.dial SSV @15:10:42.59 Allowing 130s for 25.0ml of remaining 450.0ml @15:10:42.62 Intake.select :open @15:10:44.77 Intake @15.4psia, vacuum from 9.9 to 12.9psi @15:10:44.79 SS.reconfigure :inhaling 0.47ml/s @15:10:44.83 SS.seek 25ml @15:11:32.33 Vacuum too high -- waiting up to 40 seconds @15:11:32.35 SS.reconfigure :exhale @15:11:32.39 SS.hold @15:11:42.21 Intake @14.7psia, vacuum from 9.2 to 12.2psi @15:11:42.23 SS.reconfigure :inhaling 0.43ml/s @15:11:42.27 SS.seek 25ml @15:11:51.65 Stroke completed -- waiting for pressure to equalize ... @15:11:51.66 SS.reconfigure :exhale @15:11:51.71 SS.hold @15:12:04.47 Intake.select :close @15:12:04.62 SSV.dial :exhaust @15:12:05.39 Exhaust.select :open @15:12:05.50 SS.seek 0ml @15:12:14.32 Exhaust.select :close @15:12:14.43 SSV.dial CBV @15:12:14.46 CBV.dial SSV @15:12:15.22 Allowing 130s for 25.0ml of remaining 425.0ml @15:12:15.23 Intake.select :open @15:12:17.39 Intake @15.3psia, vacuum from 9.8 to 12.8psi @15:12:17.40 SS.reconfigure :inhaling 0.43ml/s @15:12:17.45 SS.seek 25ml @15:12:47.15 recoverPRVreagent08 @15:12:47.16 PTV.dial :mixing @15:12:47.17 PBV.dial :waste1 @15:12:47.56 delay 30 seconds @15:13:12.65 Vacuum too high -- waiting up to 40 seconds @15:13:12.66 SS.reconfigure :exhale @15:13:12.70 SS.hold @15:13:17.57 PBV.dial between PSV and waste1 @15:13:17.95 PSV.dial :air @15:13:18.55 PS.reconfigure :fast @15:13:18.59 PS.seek 2.5ml @15:13:23.73 Intake @14.8psia, vacuum from 9.3 to 12.3psi @15:13:23.75 SS.reconfigure :inhaling 0.38ml/s @15:13:23.79 SS.seek 25ml @15:13:31.18 Stroke completed -- waiting for pressure to equalize ... @15:13:31.19 SS.reconfigure :exhale @15:13:31.24 SS.hold @15:13:42.54 PSV.dial :mixloop @15:13:42.98 delay 2 seconds @15:13:43.84 Intake.select :close @15:13:43.98 SSV.dial :exhaust @15:13:44.75 Exhaust.select :open @15:13:44.86 SS.seek 0ml @15:13:45.00 PSV.dial :air @15:13:45.46 delay 2 seconds @15:13:47.48 PSV.dial :mixloop @15:13:47.95 delay 2 seconds @15:13:49.96 PSV.dial :air @15:13:50.56 PS.seek 0ml @15:13:53.62 Exhaust.select :close @15:13:53.73 SSV.dial CBV @15:13:53.76 CBV.dial SSV @15:13:54.53 Allowing 130s for 25.0ml of remaining 400.0ml @15:13:54.54 Intake.select :open @15:13:56.69 Intake @15.3psia, vacuum from 9.8 to 12.8psi @15:13:56.71 SS.reconfigure :inhaling 0.38ml/s @15:13:56.76 SS.seek 25ml @15:14:13.95 washCycleDA #1 of 3 @15:14:13.96 PSR.select :air @15:14:13.97 PSV.dial PSR @15:14:15.36 Pulling 0.5ml of PSR.wash @15:14:15.37 PSR.select :wash @15:14:15.48 PS.reconfigure :default @15:14:15.52 PS.seek 0.5ml @15:14:21.54 delay 5 seconds @15:14:26.56 PSR.select :air @15:14:26.68 PS.seek 2ml @15:14:42.85 PTV.dial :puck @15:14:44.10 PSV.dial PTV @15:14:44.87 PBV.dial :waste1 @15:14:45.30 PS.seek 1.5ml @15:14:51.29 delay 3 seconds @15:14:54.31 PBV.dial between PSV and waste1 @15:14:54.69 PS.seek 0ml @15:15:06.49 Stroke completed -- waiting for pressure to equalize ... @15:15:06.50 SS.reconfigure :exhale @15:15:06.55 SS.hold @15:15:10.85 delay 2:00 @15:15:30.95 Intake.select :close @15:15:31.09 SSV.dial :exhaust @15:15:31.86 Exhaust.select :open @15:15:31.97 SS.seek 0ml @15:15:40.79 Exhaust.select :close @15:15:40.90 SSV.dial CBV @15:15:40.92 CBV.dial SSV @15:15:41.71 Allowing 130s for 25.0ml of remaining 375.0ml @15:15:41.72 Intake.select :open @15:15:43.87 Intake @15.4psia, vacuum from 9.9 to 12.9psi @15:15:43.89 SS.reconfigure :inhaling 0.38ml/s @15:15:43.94 SS.seek 25ml @15:16:35.08 Vacuum too high -- waiting up to 40 seconds @15:16:35.09 SS.reconfigure :exhale @15:16:35.13 SS.hold @15:16:47.79 Intake @14.9psia, vacuum from 9.4 to 12.4psi @15:16:47.81 SS.reconfigure :inhaling 0.34ml/s @15:16:47.85 SS.seek 25ml @15:17:08.75 Stroke completed -- waiting for pressure to equalize ... @15:17:08.77 SS.reconfigure :exhale @15:17:08.81 SS.hold @15:17:10.87 recoverPSRreagent08 @15:17:10.88 PTV.dial :puck @15:17:10.89 PBV.dial :waste1 @15:17:11.26 delay 30 seconds @15:17:29.15 Intake.select :close @15:17:29.29 SSV.dial :exhaust @15:17:30.05 Exhaust.select :open @15:17:30.16 SS.seek 0ml @15:17:38.97 Exhaust.select :close @15:17:39.08 SSV.dial CBV @15:17:39.10 CBV.dial SSV @15:17:40.40 Allowing 130s for 25.0ml of remaining 350.0ml @15:17:40.41 Intake.select :open @15:17:41.27 PBV.dial between PSV and waste1 @15:17:41.65 PSR.select :air @15:17:41.67 PSV.dial PSR @15:17:42.43 PS.reconfigure :fast @15:17:42.48 PS.seek 2.5ml @15:17:42.57 Intake @15.3psia, vacuum from 9.8 to 12.8psi @15:17:42.59 SS.reconfigure :inhaling 0.34ml/s @15:17:42.64 SS.seek 25ml @15:18:06.35 PSV.dial PTV @15:18:07.13 delay 5 seconds @15:18:12.15 PSV.dial PSR @15:18:12.91 delay 5 seconds @15:18:17.93 PSV.dial PTV @15:18:18.69 delay 5 seconds @15:18:23.71 PSV.dial :air @15:18:24.48 PS.seek 0ml @15:18:47.72 washCycleDA #2 of 3 @15:18:47.73 PSR.select :air @15:18:47.75 PSV.dial PSR @15:18:49.02 Vacuum too high -- waiting up to 40 seconds @15:18:49.03 SS.reconfigure :exhale @15:18:49.08 SS.hold @15:18:49.21 Pulling 0.5ml of PSR.wash @15:18:49.22 PSR.select :wash @15:18:49.39 PS.reconfigure :default @15:18:49.44 PS.seek 0.5ml @15:18:55.32 delay 5 seconds @15:19:00.33 PSR.select :air @15:19:00.44 PS.seek 2ml @15:19:05.33 Intake @14.9psia, vacuum from 9.4 to 12.4psi @15:19:05.35 SS.reconfigure :inhaling 0.31ml/s @15:19:05.39 SS.seek 25ml @15:19:16.63 PTV.dial :puck @15:19:16.64 PSV.dial PTV @15:19:17.39 PBV.dial :waste1 @15:19:17.83 PS.seek 1.5ml @15:19:19.97 Stroke completed -- waiting for pressure to equalize ... @15:19:19.99 SS.reconfigure :exhale @15:19:20.03 SS.hold @15:19:23.72 delay 3 seconds @15:19:26.74 PBV.dial between PSV and waste1 @15:19:27.12 PS.seek 0ml @15:19:40.32 Intake.select :close @15:19:40.46 SSV.dial :exhaust @15:19:41.22 Exhaust.select :open @15:19:41.33 SS.seek 0ml @15:19:43.29 delay 2:00 @15:19:50.41 Exhaust.select :close @15:19:50.52 SSV.dial CBV @15:19:50.54 CBV.dial SSV @15:19:51.30 Allowing 130s for 25.0ml of remaining 325.0ml @15:19:51.32 Intake.select :open @15:19:53.47 Intake @15.4psia, vacuum from 9.9 to 12.9psi @15:19:53.49 SS.reconfigure :inhaling 0.31ml/s @15:19:53.53 SS.seek 25ml @15:21:20.61 Vacuum too high -- waiting up to 40 seconds @15:21:20.62 SS.reconfigure :exhale @15:21:20.66 SS.hold @15:21:39.27 Intake @15.0psia, vacuum from 9.5 to 12.5psi @15:21:39.29 SS.reconfigure :inhaling 0.28ml/s @15:21:39.34 SS.seek 25ml @15:21:40.92 Stroke completed -- waiting for pressure to equalize ... @15:21:40.93 SS.reconfigure :exhale @15:21:40.98 SS.hold @15:21:43.34 recoverPSRreagent08 @15:21:43.35 PTV.dial :puck @15:21:43.36 PBV.dial :waste1 @15:21:43.74 delay 30 seconds @15:21:57.20 Intake.select :close @15:21:57.35 SSV.dial :exhaust @15:21:58.11 Exhaust.select :open @15:21:58.22 SS.seek 0ml @15:22:07.05 Exhaust.select :close @15:22:07.15 SSV.dial CBV @15:22:07.21 CBV.dial SSV @15:22:07.93 Allowing 130s for 25.0ml of remaining 300.0ml @15:22:07.94 Intake.select :open @15:22:10.09 Intake @15.4psia, vacuum from 9.9 to 12.9psi @15:22:10.11 SS.reconfigure :inhaling 0.28ml/s @15:22:10.16 SS.seek 25ml @15:22:13.75 PBV.dial between PSV and waste1 @15:22:14.13 PSR.select :air @15:22:14.15 PSV.dial PSR @15:22:14.91 PS.reconfigure :fast @15:22:14.96 PS.seek 2.5ml @15:22:38.87 PSV.dial PTV @15:22:39.77 delay 5 seconds @15:22:44.79 PSV.dial PSR @15:22:45.55 delay 5 seconds @15:22:50.57 PSV.dial PTV @15:22:51.33 delay 5 seconds @15:22:56.35 PSV.dial :air @15:22:57.12 PS.seek 0ml @15:23:20.38 washCycleDA #3 of 3 @15:23:20.39 PSR.select :air @15:23:20.40 PSV.dial PSR @15:23:21.79 Pulling 0.5ml of PSR.wash @15:23:21.80 PSR.select :wash @15:23:21.91 PS.reconfigure :default @15:23:21.95 PS.seek 0.5ml @15:23:27.94 delay 5 seconds @15:23:32.96 PSR.select :air @15:23:33.07 PS.seek 2ml @15:23:49.15 PTV.dial :puck @15:23:49.17 PSV.dial PTV @15:23:49.92 PBV.dial :waste1 @15:23:50.36 PS.seek 1.5ml @15:23:50.67 Vacuum too high -- waiting up to 40 seconds @15:23:50.68 SS.reconfigure :exhale @15:23:50.72 SS.hold @15:23:56.27 delay 3 seconds @15:23:59.30 PBV.dial between PSV and waste1 @15:23:59.68 PS.seek 0ml @15:24:09.65 Intake @15.1psia, vacuum from 9.6 to 12.6psi @15:24:09.67 SS.reconfigure :inhaling 0.25ml/s @15:24:09.71 SS.seek 25ml @15:24:10.48 Stroke completed -- waiting for pressure to equalize ... @15:24:10.49 SS.reconfigure :exhale @15:24:10.54 SS.hold @15:24:15.97 delay 2:00 @15:24:17.95 SS.reconfigure :default @15:24:18.00 SS.hold @15:24:18.11 Intake.select :close @15:24:18.26 ShallowSampler::Clogged in WCR -- Filtering too Slowly after 725.0ml @15:24:18.27 SSV.dial :exhaust @15:24:19.03 Exhaust.select :open @15:24:19.14 SS.reconfigure :exhale @15:24:19.18 SS.seek 0ml @15:24:28.11 Exhaust.select :close @15:24:28.22 Sampled 725.0ml @15:24:28.24 Drying puck before fixing for 20:00 CBV.dial :waste2b @15:24:29.00 delay 1:00 @15:25:29.03 CSV.dial :air @15:25:29.49 CS.reconfigure :fast @15:25:29.54 CS.seek 10ml @15:25:53.72 Pumping wcr puck from tube 2 up to 35psi... @15:25:53.73 CBV.dial SSV @15:25:55.05 CBV.dial between CSV and waste2b @15:25:56.01 CSV.dial between air and CTV @15:25:56.35 CS.seek 7.345ml @15:26:03.22 CTV.dial CSV,avoiding: :air @15:26:03.24 CSV.dial CTV @15:26:04.05 CS.reconfigure :pressurize @15:26:04.10 CS.seek 0ml @15:26:16.00 recoverPSRreagent08 @15:26:16.01 PTV.dial :puck @15:26:16.02 PBV.dial :waste1 @15:26:16.39 delay 30 seconds @15:26:17.24 CBV.dial :waste2b @15:26:17.58 CS.reconfigure :default @15:26:17.63 CS.seek 0ml @15:26:24.50 delay 1:00 @15:26:46.40 PBV.dial between PSV and waste1 @15:26:46.78 PSR.select :air @15:26:46.80 PSV.dial PSR @15:26:47.56 PS.reconfigure :fast @15:26:47.61 PS.seek 2.5ml @15:27:11.52 PSV.dial PTV @15:27:12.30 delay 5 seconds @15:27:17.32 PSV.dial PSR @15:27:18.06 delay 5 seconds @15:27:23.08 PSV.dial PTV @15:27:23.84 delay 5 seconds @15:27:24.52 CTV.dial :air,avoiding: :intake @15:27:25.86 Fix #1 of 2 (with RNAlater) for 10:00 @15:27:25.88 CSV.dial CSR @15:27:26.37 Pulling 2ml of CSR.RNAlater @15:27:26.38 CSR.select :RNAlater @15:27:26.49 CS.seek 2ml @15:27:28.86 PSV.dial :air @15:27:29.63 PS.seek 0ml @15:27:32.44 delay 5 seconds @15:27:37.46 CSR.select :air @15:27:37.56 CS.reconfigure :fast @15:27:37.60 CS.seek 10ml @15:27:52.74 Gate.power :camera,:ON @15:27:52.76 Mixing DA Substrates @15:27:52.77 PSR.select :air @15:27:52.79 PSV.dial PSR @15:27:54.34 Pulling 0.25ml of PSR.sub1 @15:27:54.37 PSR.select :sub1 @15:27:54.59 PS.reconfigure :default @15:27:54.72 PS.seek 0.25ml @15:27:57.25 Pumping wcr puck from tube 2 up to 35psi... @15:27:57.26 CBV.dial SSV @15:27:58.12 delay 5 seconds @15:27:58.59 CBV.dial between CSV and waste2b @15:28:00.09 CSV.dial between air and CTV @15:28:00.71 CS.seek 7.292ml @15:28:03.19 Pulling 0.25ml of PSR.sub2 @15:28:03.20 PSR.select :sub2 @15:28:03.31 PS.seek 0.5ml @15:28:06.65 delay 5 seconds @15:28:07.85 CTV.dial CSV,avoiding: :air @15:28:07.88 CSV.dial CTV @15:28:09.30 CS.reconfigure :pressurize @15:28:09.35 CS.seek 0ml @15:28:11.67 PSR.select :air @15:28:11.78 PS.seek 2ml @15:28:24.33 delay 10:00 @15:28:27.93 PTV.dial :puck @15:28:27.94 PSV.dial PTV @15:28:28.70 PBV.dial :waste1 @15:28:29.13 PS.seek 1.5ml @15:28:34.96 delay 3 seconds @15:28:37.98 PBV.dial between PSV and waste1 @15:28:38.36 PS.seek 0ml @15:28:54.71 delay 10 seconds @15:29:04.74 recoverPSRreagent08 @15:29:04.75 PTV.dial :puck @15:29:04.76 PBV.dial :waste1 @15:29:05.12 delay 30 seconds @15:29:35.14 PBV.dial between PSV and waste1 @15:29:35.51 PSR.select :air @15:29:35.53 PSV.dial PSR @15:29:36.31 PS.reconfigure :fast @15:29:36.36 PS.seek 2.5ml @15:30:00.49 PSV.dial PTV @15:30:01.26 delay 5 seconds @15:30:06.28 PSV.dial PSR @15:30:07.04 delay 5 seconds @15:30:12.06 PSV.dial PTV @15:30:12.84 delay 5 seconds @15:30:17.86 PSV.dial :air @15:30:18.75 PS.seek 0ml @15:30:42.21 Additional air clear @15:30:42.22 PSR.select :air @15:30:42.24 PSV.dial PSR @15:30:43.64 Pulling 2ml of PSR.air @15:30:43.65 PSR.select :air @15:30:43.67 PS.reconfigure :default @15:30:43.71 PS.seek 2ml @15:31:05.18 delay 5 seconds @15:31:10.20 PTV.dial :puck @15:31:10.21 PSV.dial PTV @15:31:10.99 PBV.dial :waste1 @15:31:11.43 PS.seek 1.5ml @15:31:17.33 delay 3 seconds @15:31:20.35 PBV.dial between PSV and waste1 @15:31:20.73 delay 1 second @15:31:21.75 PSV.dial :waste1 @15:31:22.81 PS.seek 0ml @15:31:39.01 Processing Puck Heater at 28C before imaging @15:31:39.02 PH.coast @15:31:39.14 Imaging da puck in Processing Clamp while storing it to tube 1 @15:31:39.30 SC.reconfigure :tight @15:31:39.35 SC.seek 1 @15:31:43.05 SC.configure :limp @15:31:43.24 Elbow.reconfigure :tight @15:31:43.29 Elbow.seek 1 @15:31:45.02 Forearm.reconfigure :tight @15:31:45.07 Forearm.seek 1 + 450 counts @15:31:46.13 SE.reconfigure :pick @15:31:46.18 SE.seek :beamBreak @15:31:47.41 SE.configure :default @15:31:47.56 Elbow.reconfigure :limp @15:31:47.60 Forearm.reconfigure :loose @15:31:47.65 Forearm.seek 1 @15:31:47.91 Elbow.hold @15:31:47.97 Elbow.reconfigure :tight @15:31:48.07 SE.seek 29.07 Pucks below Top Plate @15:31:48.60 Forearm.force -20 @15:31:48.63 Forearm.seek 1 + 310 counts @15:31:48.91 Forearm.reconfigure :tight @15:31:48.96 Forearm.seek 1 + 450 counts @15:31:49.13 SE.seek 30.07 Pucks below Top Plate @15:31:49.73 Forearm.reconfigure :loose @15:31:49.77 Forearm.seek :retract @15:31:50.70 Elbow.seek PC @15:31:53.51 Elbow.reconfigure :loose @15:31:53.60 Forearm.seek PC @15:31:55.05 Hand.close @15:31:55.63 PC.seek :open @15:32:00.40 Forearm.seek :retract @15:32:01.34 Elbow.reconfigure :tight @15:32:01.39 Elbow.seek 1 @15:32:04.05 Forearm.reconfigure :tight @15:32:04.10 Forearm.seek 1 + 450 counts @15:32:05.10 Hand.open @15:32:05.82 SE.seek 29.72 Pucks below Top Plate @15:32:06.08 SE.reconfigure :seal @15:32:06.13 SE.seek 28.76 Pucks below Top Plate @15:32:06.66 Lights, camera, ACTION! @15:32:06.69 PuckCamera: snap -bin=2,2 -auto /var/log/esp/da22dec2015h850ml.tif @15:32:26.18 Waiting >20 seconds for da to relinquish FlushPuck @15:32:27.71 SX HX9/16 (USB:08): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 17.708 seconds /var/log/esp/da22dec2015h850ml.tif: TIFF Upload Complete @15:32:27.74 PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da22dec2015h850ml15s.tif @15:32:43.39 SX HX9/16 (USB:08): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15 seconds /var/log/esp/midres/da22dec2015h850ml15s.tif: TIFF Upload Complete @15:32:43.41 PuckCamera: snap -bin=2,2 20.0 /var/log/esp/midres/da22dec2015h850ml20s.tif @15:33:04.03 SX HX9/16 (USB:08): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 20 seconds /var/log/esp/midres/da22dec2015h850ml20s.tif: TIFF Upload Complete @15:33:04.05 Gate.power :camera,:OFF @15:33:04.06 DAfans.email "17.708 sec auto-exposure",Subject: "da22dec2015h850ml images!" @15:33:04.32 Elbow.seek 1 @15:33:04.51 Forearm.seek 1 + 450 counts @15:33:04.59 SE.reconfigure :gently @15:33:04.65 SE.seek 29.47 Pucks below Top Plate @15:33:05.30 Elbow.reconfigure :limp @15:33:05.35 Forearm.reconfigure :loose @15:33:05.39 Forearm.seek 1 @15:33:05.65 Elbow.hold @15:33:05.72 Elbow.reconfigure :tight @15:33:05.77 SE.reconfigure :default @15:33:05.82 SE.seek 29.66 Pucks below Top Plate @15:33:06.27 Forearm.force -20 @15:33:06.31 Forearm.seek 1 + 290 counts @15:33:06.60 Forearm.reconfigure :tight @15:33:06.67 Forearm.seek 1 + 450 counts @15:33:06.98 SE.seek :down @15:33:07.68 SE.stop @15:33:08.11 Forearm.reconfigure :loose @15:33:08.15 Forearm.seek :retract @15:33:09.17 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @15:33:09.38 Elbow.seek FlushPuck::Garage @15:33:11.36 Elbow.reconfigure :loose @15:33:11.55 Forearm.seek :garage @15:33:12.61 Hand.close @15:33:13.58 Forearm.seek :retract @15:33:14.61 Elbow.reconfigure :tight @15:33:14.70 Elbow.seek PC @15:33:16.11 Elbow.reconfigure :loose @15:33:16.30 Forearm.reconfigure :tight @15:33:16.39 Forearm.seek PC @15:33:18.01 PC.seek :closed @15:33:22.76 Processing Clamp onSpring/offSpring for 16/26 samples, on/off torque 1.57 @15:33:22.91 Hand.open @15:33:24.77 Forearm.reconfigure :loose @15:33:24.86 Forearm.seek :retract @15:33:25.86 PSR.select :air @15:33:25.92 PSV.dial PSR @15:33:26.53 Pulling 0.5ml of PSR.flush @15:33:26.55 PSR.select :flush @15:33:26.78 PS.seek 0.5ml @15:33:32.83 delay 5 seconds @15:33:37.88 PSR.select :air @15:33:38.03 PS.seek 2.5ml @15:33:38.60 upsync @15:33:59.33 delay 1 second @15:34:00.35 PRV.dial :waste2,avoiding: :ab2 @15:34:02.22 PTV.dial :PRVmixing @15:34:03.55 PSV.dial :mixloop @15:34:04.60 PS.seek :empty @15:34:31.02 PSR.select :air @15:34:31.04 PSV.dial PSR @15:34:32.15 Pulling 0.5ml of PSR.flush @15:34:32.16 PSR.select :flush @15:34:32.28 PS.seek 0.5ml @15:34:38.24 delay 5 seconds @15:34:43.26 PSR.select :air @15:34:43.38 PS.seek 2.5ml @15:35:04.58 delay 1 second @15:35:05.60 PBV.dial :waste1 @15:35:05.97 PTV.dial :mixing @15:35:06.72 PSV.dial :mixloop @15:35:07.79 PS.seek :empty @15:35:34.11 PSV.dial PSR @15:35:35.16 PSR.select :flush @15:35:35.27 PS.seek 1ml @15:35:46.36 PSR.select :air @15:35:46.47 PS.seek 2.5ml @15:36:02.77 PSV.dial PTV @15:36:03.53 PTV.dial :puck @15:36:04.28 PBV.dial :waste1 @15:36:04.30 PS.seek 0ml @15:36:30.67 PSV.dial PSR @15:36:31.44 PSR.select :flush @15:36:31.55 PS.seek 0.5ml @15:36:37.42 PSR.select :air @15:36:37.53 PS.seek 2.5ml @15:36:58.98 PSV.dial PTV @15:36:59.75 PBV.dial PSV @15:37:00.33 PS.seek 0ml @15:37:26.73 PSV.dial PBV @15:37:27.21 PS.seek 2.5ml @15:37:53.58 PSV.dial :waste1 @15:37:54.33 PS.seek 0ml @15:38:20.70 PSV.dial PSR @15:38:21.17 PSR.select :air @15:38:21.20 PS.seek 2.5ml @15:38:24.35 CBV.dial :waste2b @15:38:24.68 CS.reconfigure :default @15:38:24.73 CS.seek 0ml @15:38:29.77 delay 1:00 @15:38:47.51 PSV.dial PTV @15:38:48.26 PBV.dial PSV @15:38:48.28 PS.seek 0ml @15:39:14.67 PSV.dial PBV @15:39:15.15 PS.seek 2.5ml @15:39:29.79 CTV.dial :air,avoiding: :intake @15:39:31.12 Fix #2 of 2 (with RNAlater) for 10:00 @15:39:31.13 CSV.dial CSR @15:39:31.62 Pulling 2ml of CSR.RNAlater @15:39:31.63 CSR.select :RNAlater @15:39:31.74 CS.seek 2ml @15:39:37.62 delay 5 seconds @15:39:41.51 PSV.dial :waste1 @15:39:42.27 PS.seek 0ml @15:39:42.63 CSR.select :air @15:39:42.74 CS.reconfigure :fast @15:39:42.78 CS.seek 10ml @15:40:02.52 Pumping wcr puck from tube 2 up to 35psi... @15:40:02.53 CBV.dial SSV @15:40:03.83 CBV.dial between CSV and waste2b @15:40:04.78 CSV.dial between air and CTV @15:40:05.39 CS.seek 7.231ml @15:40:08.82 PSV.dial PSR @15:40:09.30 PSR.select :flush @15:40:09.43 PS.seek 1ml @15:40:12.56 CTV.dial CSV,avoiding: :air @15:40:12.58 CSV.dial CTV @15:40:14.43 CS.reconfigure :pressurize @15:40:14.48 CS.seek 0ml @15:40:20.54 PSR.select :air @15:40:20.66 PS.seek 2.5ml @15:40:32.95 delay 10:00 @15:40:36.82 PSV.dial PTV @15:40:37.68 PTV.dial :puck @15:40:37.70 PBV.dial :waste1 @15:40:38.27 PS.seek 0ml @15:41:04.64 PSV.dial PSR @15:41:05.39 PSR.select :flush @15:41:05.50 PS.seek 0.5ml @15:41:11.76 PSR.select :air @15:41:11.87 PS.seek 2.5ml @15:41:33.07 PSV.dial PTV @15:41:33.84 PBV.dial PSV @15:41:34.41 PS.seek 0ml @15:42:00.76 PSV.dial PBV @15:42:01.24 PS.seek 2.5ml @15:42:27.60 PSV.dial :waste1 @15:42:28.36 PS.seek 0ml @15:42:54.91 PSV.dial PSR @15:42:55.38 PSR.select :air @15:42:55.41 PS.seek 2.5ml @15:43:21.73 PSV.dial PTV @15:43:22.48 PBV.dial PSV @15:43:22.50 PS.seek 0ml @15:43:48.88 PSV.dial PBV @15:43:49.36 PS.seek 2.5ml @15:44:15.76 PSV.dial :waste1 @15:44:16.51 PS.seek 0ml @15:44:42.92 Garaging FlushPuck in Processing Clamp @15:44:42.99 Elbow.reconfigure :tight @15:44:43.03 Elbow.seek PC @15:44:43.14 Elbow.reconfigure :loose @15:44:43.23 Forearm.seek PC @15:44:44.16 Hand.close @15:44:44.75 PC.seek :open @15:44:49.46 Forearm.seek :retract @15:44:50.42 Elbow.reconfigure :tight @15:44:50.46 Elbow.seek :garage @15:44:51.83 Elbow.reconfigure :loose @15:44:51.93 Forearm.reconfigure :tight @15:44:51.97 Forearm.seek :garage @15:44:52.98 Hand.open @15:44:53.68 Forearm.reconfigure :loose @15:44:53.72 Forearm.seek :retract @15:50:32.97 CBV.dial :waste2b @15:50:33.29 CS.reconfigure :default @15:50:33.34 CS.seek 0ml @15:50:34.48 delay 1:00 @15:51:34.50 CTV.dial :air,avoiding: :intake @15:51:35.88 Storing wcr puck in Collection Clamp to tube 1 @15:51:36.04 SC.reconfigure :tight @15:51:36.09 SC.seek 1 @15:51:36.15 SC.configure :limp @15:51:36.35 Elbow.reconfigure :tight @15:51:36.39 Elbow.seek 1 @15:51:38.28 Forearm.reconfigure :tight @15:51:38.32 Forearm.seek 1 + 450 counts @15:51:39.33 SE.reconfigure :pick @15:51:39.43 SE.seek :beamBreak @15:51:40.57 SE.configure :default @15:51:40.71 Elbow.reconfigure :limp @15:51:40.76 Forearm.reconfigure :loose @15:51:40.81 Forearm.seek 1 @15:51:41.05 Elbow.hold @15:51:41.12 Elbow.reconfigure :tight @15:51:41.21 SE.seek 30.07 Pucks below Top Plate @15:51:41.67 Forearm.force -20 @15:51:41.71 Forearm.seek 1 + 308 counts @15:51:42.45 Forearm.reconfigure :tight @15:51:42.51 Forearm.seek 1 + 450 counts @15:51:42.70 SE.seek 31.07 Pucks below Top Plate @15:51:43.26 Forearm.reconfigure :loose @15:51:43.31 Forearm.seek :retract @15:51:44.26 Elbow.seek CC @15:51:45.88 Elbow.reconfigure :loose @15:51:45.98 Forearm.seek CC @15:51:46.94 Hand.close @15:51:47.49 CC.seek :open @15:51:52.22 Forearm.seek :retract @15:51:53.19 Elbow.reconfigure :tight @15:51:53.24 Elbow.seek 1 @15:51:55.03 Elbow.reconfigure :limp @15:51:55.08 Forearm.seek 1 @15:51:56.08 Elbow.hold @15:51:56.15 Elbow.reconfigure :tight @15:51:56.19 Hand.open @15:51:56.98 Forearm.force -20 @15:51:57.02 Forearm.seek 1 + 309 counts @15:51:57.31 Forearm.reconfigure :tight @15:51:57.36 Forearm.seek 1 + 450 counts @15:51:57.59 SE.seek :down @15:51:58.19 SE.stop @15:51:58.71 Forearm.reconfigure :loose @15:51:58.76 Forearm.seek :retract @15:51:59.68 Starting finalPurge @15:51:59.69 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @15:51:59.88 Elbow.seek FlushPuck::Garage @15:52:01.76 Elbow.reconfigure :loose @15:52:01.85 Forearm.seek :garage @15:52:02.85 Hand.close @15:52:03.60 Forearm.seek :retract @15:52:04.58 Elbow.reconfigure :tight @15:52:04.62 Elbow.seek CC @15:52:07.62 Elbow.reconfigure :loose @15:52:07.71 Forearm.reconfigure :tight @15:52:07.75 Forearm.seek CC @15:52:08.99 CC.seek :closed @15:52:13.51 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.25 @15:52:13.57 Hand.open @15:52:14.18 Forearm.reconfigure :loose @15:52:14.22 Forearm.seek :retract @15:52:15.16 Flushing the Collection Valve::Manifold @15:52:15.28 CSV.dial CSR @15:52:15.76 Pulling 5ml of CSR.flush @15:52:15.77 CSR.select :flush @15:52:15.88 CS.seek 5ml @15:52:29.88 delay 5 seconds @15:52:34.90 CSR.select :air @15:52:35.05 CS.reconfigure :fast @15:52:35.09 CS.seek 7.501ml @15:52:41.85 CSV.dial :waste @15:52:43.13 CS.reconfigure :default @15:52:43.18 CS.seek 0ml @15:53:03.13 Flushing lower manifold's waste1 port @15:53:03.25 CSV.dial :flush @15:53:03.68 CS.reconfigure :fast @15:53:03.72 CS.seek 5ml @15:53:16.57 delay 5 seconds @15:53:21.59 CSV.dial :air @15:53:22.21 CS.seek 6.999ml @15:53:28.19 CSV.dial CTV @15:53:28.22
CTV.dial CSV @15:53:29.60 CBV.dial :waste1 @15:53:30.36 CS.seek 0ml @15:53:47.01 Flushing lower manifold's waste2 port @15:53:47.12 CSV.dial :flush @15:53:47.86 CS.seek 5ml @15:54:00.47 delay 5 seconds @15:54:05.49 CSV.dial :air @15:54:06.00 CS.seek 7.001ml @15:54:11.51 CSV.dial CTV @15:54:11.54
CTV.dial CSV @15:54:11.96 CBV.dial :waste2 @15:54:12.99 CS.seek 0ml @15:54:29.66 Flushing lower manifold's waste2b port @15:54:29.78 CSV.dial :flush @15:54:30.51 CS.seek 5ml @15:54:43.22 delay 5 seconds @15:54:48.24 CSV.dial :air @15:54:48.75 CS.seek 7ml @15:54:54.29 CSV.dial CTV @15:54:54.32
CTV.dial CSV @15:54:54.74 CBV.dial :waste2b @15:54:55.79 CS.seek 0ml @15:55:12.63 Disinfecting external Intake with kill @15:55:12.74 CSV.dial CSR @15:55:13.23 Pulling 8ml of CSR.kill @15:55:13.24 CSR.select :kill @15:55:13.35 CS.seek 8ml @15:55:33.13 delay 5 seconds @15:55:38.15 CSR.select :air @15:55:38.26 CS.seek 10ml @15:55:43.78 CSV.dial CBV @15:55:43.83
CBV.dial CSV @15:55:44.85 CTV.dial :intake @15:55:45.63 Intake.select :open @15:55:45.73 CS.seek 2ml @15:56:04.62 Intake.select :close @15:56:04.72 CTV.dial :air @15:56:05.49 CS.reconfigure :default @15:56:05.53 CS.seek 0ml @15:56:11.50 CSV.dial CSR @15:56:12.68 Pulling 5ml of CSR.flush @15:56:12.69 CSR.select :flush @15:56:12.80 CS.seek 5ml @15:56:26.72 delay 5 seconds @15:56:31.74 CSR.select :air @15:56:31.85 CS.seek 10ml @15:56:45.41 CSV.dial CBV @15:56:46.52 CS.seek 0ml @15:57:12.94 Garaging FlushPuck in Collection Clamp @15:57:13.01 Elbow.reconfigure :tight @15:57:13.05 Elbow.seek CC @15:57:13.48 Elbow.reconfigure :loose @15:57:13.57 Forearm.seek CC @15:57:14.53 Hand.close @15:57:15.11 CC.seek :open @15:57:19.73 Forearm.seek :retract @15:57:20.67 Elbow.reconfigure :tight @15:57:20.71 Elbow.seek :garage @15:57:23.72 Elbow.reconfigure :loose @15:57:23.81 Forearm.reconfigure :tight @15:57:23.85 Forearm.seek :garage @15:57:24.87 Hand.open @15:57:25.55 Forearm.reconfigure :loose @15:57:25.59 Forearm.seek :retract @15:57:26.53 Completed [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] begun 13:19:56 @15:57:26.58 Asleep after completing DA_WCR_122022 assays @15:57:26.60 GoodNews.email "{1 => 32,\n 2 => 1,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@15:49:05, 19% flow, 14.7C, 28% humidity, 14.5psia, 13.749V, 0.417A, 0.405A avg, 5.74W\nSampled 725.0ml",Subject: "Asleep after completing DA_WCR_122022 assays" @15:57:32.12 upsync @13:21:19.97UTC03-Jan-23
ESP2 v3.41 I2C Gateway v3.20 for core bus @13:21:22.63 Configuration for short puck jake 1/11/21 by brent@mbari.org /home/esp/esp2/type/shallow/jake/configure.rb @13:21:26.69 Polling Can every 10:00 @13:21:26.77 Cleanly exited at 13:18:53UTC03-Jan-23 @13:21:26.81 Loading /home/esp/esp2/mission/DA_WCR_1323.rb mission @13:21:26.86 Preparing to sleep until + 00:00:05 @13:21:26.88 GoodNews.email "Starting DA_WCR_1323 w/pucks in tube 2\n{1 => 32,\n 2 => 1,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@13:21:26, 18% flow, 15.1C, 30% humidity, 14.6psia, 13.759V, 0.333A, 0.315A avg, 4.58W",Subject: "DA_WCR_1323 begun" @13:21:26.92
Asleep til + 00:00:05 @13:21:26.96 Continuing at 13:21 @13:21:27.20 Gate.power :core,:OFF @13:21:27.22 Gate.power :raw,:OFF @13:21:27.23 Shutdown @13:21:31.60 GoodNews.email "Then, in tube 2: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2/mission/DA_WCR_1323.rb:14\n{1 => 32,\n 2 => 1,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@13:21:26, 18% flow, 15.1C, 30% humidity, 14.6psia, 13.759V, 0.333A, 0.315A avg, 4.58W",Subject: "Asleep until 13:21" @13:21:31.90
Powering core bus @13:21:33.86 upsync @13:21:37.36
Gate.power :raw,:ON @13:21:37.51 upsync @13:21:38.33
CS.configure :default @13:21:38.42 PS.configure :default @13:21:38.51 SS.configure :default @13:21:38.60 CC.configure :default @13:21:38.68 PC.configure :default @13:21:38.79 Forearm.configure :loose @13:21:38.87 Elbow.configure :loose @13:21:38.98 SC.configure :loose @13:21:39.06 SE.configure :default @13:21:39.10 CH.coast @13:21:39.15 CH.configure :default @13:21:39.18 PH.coast @13:21:39.24 PH.configure :default @13:21:39.27 All dwarf controllers running firmware version 3.85 @13:21:39.37 Valve.reset @13:21:39.57 Intake.select :close @13:21:39.66 Exhaust.select :close @13:21:39.68 CTV.dial :air @13:21:39.76 PSV.dial :air @13:21:39.85
Forearm.reconfigure :loose @13:21:39.91 Forearm.home.seek :retract @13:21:39.93 CSV.dial :waste1 @13:21:40.53 SSV.dial :spit,avoiding: :exhaust @13:21:40.72 Hand.close @13:21:40.76
Elbow.jog 1500 @13:21:40.85 PS.reconfigure :default @13:21:40.90 PS.home.seek 0ml @13:21:41.36 CS.reconfigure :fast @13:21:41.39 SS.reconfigure :default @13:21:41.48 CS.home.seek 0ml @13:21:41.51 SS.home.seek 0ml @13:21:41.62
Elbow.reconfigure :loose @13:21:41.66 Elbow.home.seek :clear @13:21:44.87 SE.reconfigure :default @13:21:44.91 SE.home.seek :down @13:21:45.51 SC.reconfigure :tight @13:21:45.55 SC.home.seek :initial @13:21:49.18 PS.stop @13:22:13.99
SC.configure :limp @13:22:14.04 Forearm.seek :retract @13:22:14.09 Elbow.seek :hammer @13:22:15.12 Forearm.reconfigure :tight @13:22:15.16 Forearm.seek retracted @13:22:15.51 PC.reconfigure :default @13:22:15.53
CC.reconfigure :default @13:22:15.61 PC.home.seek :open @13:22:15.64
CC.home.seek :open @13:22:19.14 Phase [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] @13:22:19.16 Notice.email "At 13:22:19 began in tube 2: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2/mission/DA_WCR_1323.rb:14\n{1 => 32,\n 2 => 1,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@13:22:19, 19% flow, 15.1C, 30% humidity, 14.6psia, 13.734V, 0.399A, 0.387A avg, 5.48W",Subject: "Started DA_WCR_1323 phase initialPurge; da [1000, 1000] #da and wsr are run simultanelously" @13:22:19.18
Starting initialPurge @13:22:19.20 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @13:22:19.26 Hand.open @13:22:19.95 Elbow.reconfigure :tight @13:22:20.01 Elbow.seek FlushPuck::Garage @13:22:21.98 Elbow.reconfigure :loose @13:22:22.07 Forearm.reconfigure :loose @13:22:22.11 Forearm.seek :garage @13:22:23.09 Hand.close @13:22:23.84 Forearm.seek :retract @13:22:24.81 Elbow.reconfigure :tight @13:22:24.86 Elbow.seek CC @13:22:25.13 upsync @13:22:27.86
Elbow.reconfigure :loose @13:22:27.96 Forearm.reconfigure :tight @13:22:28.00 Forearm.seek CC @13:22:29.25 CC.seek :closed @13:22:33.80 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.37 @13:22:33.87 Hand.open @13:22:34.52 Forearm.reconfigure :loose @13:22:34.57 Forearm.seek :retract @13:22:35.53 Flushing the Collection Valve::Manifold @13:22:35.65 CSV.dial CSR @13:22:37.11 Pulling 5ml of CSR.flush @13:22:37.12 CSR.select :flush @13:22:37.23 CS.reconfigure :default @13:22:37.27 CS.seek 5ml @13:22:50.85 delay 5 seconds @13:22:55.87 CSR.select :air @13:22:56.02 CS.reconfigure :fast @13:22:56.07 CS.seek 7.5ml @13:23:02.79 CSV.dial :waste @13:23:04.06 CS.reconfigure :default @13:23:04.10 CS.seek 0ml @13:23:24.13 Purging external Intake @13:23:24.25 CSV.dial :flush @13:23:24.68 CS.reconfigure :fast @13:23:24.73 CS.seek 10ml @13:23:49.19 delay 5 seconds @13:23:54.21 CSV.dial CBV @13:23:54.24
CBV.dial CSV @13:23:54.99 CTV.dial :intake @13:23:55.76 Intake.select :open @13:23:55.86 CS.seek 0ml @13:24:19.44 Intake.select :close @13:24:19.54 CTV.dial :air @13:24:20.34 Flushing upper manifold @13:24:20.45 CSV.dial :flush @13:24:21.19 CS.seek 5ml @13:24:33.82 delay 5 seconds @13:24:38.84 CSV.dial :air @13:24:39.33 CS.seek 7.001ml @13:24:44.79 CSV.dial CBV @13:24:44.83
CBV.dial CSV @13:24:45.82 CTV.dial :air @13:24:45.84 CS.seek 0ml @13:25:02.69 delay 7 seconds @13:25:09.72 CS.seek 3ml @13:25:17.91 CSV.dial :waste @13:25:18.36 CS.seek 0ml @13:25:26.14 CSV.dial :flush @13:25:26.58 CS.seek 3ml @13:25:34.47 delay 5 seconds @13:25:39.49 CSV.dial :air @13:25:40.11 CS.seek 5ml @13:25:45.63 CSV.dial :air @13:25:45.65 CS.seek 0ml @13:25:57.94 delay 7 seconds @13:26:04.97 CS.seek 3ml @13:26:12.97 CSV.dial :waste @13:26:13.68 CS.seek 0ml @13:26:21.44 Garaging FlushPuck in Collection Clamp @13:26:21.51 Elbow.reconfigure :tight @13:26:21.56 Elbow.seek CC @13:26:21.97 Elbow.reconfigure :loose @13:26:22.06 Forearm.seek CC @13:26:23.05 Hand.close @13:26:23.71 CC.seek :open @13:26:28.33 Forearm.seek :retract @13:26:29.31 Elbow.reconfigure :tight @13:26:29.35 Elbow.seek :garage @13:26:32.36 Elbow.reconfigure :loose @13:26:32.45 Forearm.reconfigure :tight @13:26:32.49 Forearm.seek :garage @13:26:33.45 Hand.open @13:26:34.21 Forearm.reconfigure :loose @13:26:34.26 Forearm.seek :retract @13:26:35.20 da sampling at most 1000ml, wcr at most 1000ml @13:26:35.23 Loading sh1 puck from tube 2 into Collection Clamp @13:26:35.24 DAfans.email "Started da in tube 2 at 13:26:35UTC03-Jan-23\n{1 => 32,\n 2 => 1,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@13:22:19, 19% flow, 15.1C, 30% humidity, 14.6psia, 13.734V, 0.399A, 0.387A avg, 5.48W",Subject: "da sampling at most 1000ml, wcr at most 1000ml" @13:26:35.50
SC.reconfigure :tight @13:26:35.54 SC.seek 2 @13:26:41.03 upsync @13:26:42.27
SC.configure :limp @13:26:42.48 Elbow.reconfigure :tight @13:26:42.52 Elbow.seek 2 @13:26:44.49 Forearm.reconfigure :tight @13:26:44.53 Forearm.seek 2 + 450 counts @13:26:45.51 SE.reconfigure :pick @13:26:45.57 SE.seek :beamBreak @13:26:48.69 SE.configure :default @13:26:48.83 Elbow.seek 2 @13:26:49.00 Forearm.seek 2 + 300 counts @13:26:49.27 Forearm.reconfigure :loose @13:26:49.31 Hand.close @13:26:49.98 SE.seek :down @13:26:50.17
Forearm.seek :retract @13:26:51.09 Elbow.seek CC @13:26:52.73 Elbow.reconfigure :loose @13:26:52.74 SE.stop @13:26:52.87
Forearm.reconfigure :tight @13:26:52.92 Forearm.seek CC @13:26:54.13 CC.seek :closed @13:26:58.66 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.25 @13:26:58.67 Hand.open @13:26:59.39 Forearm.reconfigure :loose @13:26:59.44 Forearm.seek :retract @13:27:00.40 collectSample 1000ml w/bubble point of 24psi @13:27:00.41 Priming 7ml @13:27:00.42 Intake.select :close @13:27:00.43 Exhaust.select :close @13:27:00.44 SS.hold @13:27:00.65 Calibrating pressure and temperature sensors @13:27:00.67 CTV.dial :air @13:27:00.69 SSV.dial :spit,avoiding: :exhaust @13:27:00.71 SS.reconfigure :fast @13:27:00.75 SS.seek 5ml @13:27:03.60 SSV.dial CBV @13:27:03.63
CBV.dial SSV @13:27:04.92 delay 3.8 seconds @13:27:08.84 SSV.dial :spit @13:27:10.16 SS.seek 0ml @13:27:12.91 SSV.dial CBV @13:27:14.19 CTV.dial :intake @13:27:14.99 SSV.dial CBV @13:27:15.01
CBV.dial SSV @13:27:15.03 Allowing 40s for 7.0ml of remaining 7.0ml @13:27:15.04 Intake.select :open @13:27:17.19 Intake @15.2psia, vacuum from 9.7 to 12.7psi @13:27:17.21 SS.reconfigure :inhaling 2.20ml/s @13:27:17.25 SS.seek 7ml @13:27:24.03 Stroke completed -- waiting for pressure to equalize ... @13:27:24.04 SS.reconfigure :exhale @13:27:24.08 SS.hold @13:27:26.03 Intake.select :close @13:27:26.17 SSV.dial :exhaust @13:27:26.91 Exhaust.select :open @13:27:27.01 SS.seek 0ml @13:27:30.89 Exhaust.select :close @13:27:30.99 Sampling 1000.0ml @13:27:31.00 Intake.select :close @13:27:31.01 Exhaust.select :close @13:27:31.02 SS.reconfigure :default @13:27:31.06 SS.hold @13:27:31.24 CTV.dial :intake @13:27:31.26 SSV.dial CBV @13:27:31.31
CBV.dial SSV @13:27:32.03 Allowing 130s for 25.0ml of remaining 1000.0ml @13:27:32.04 Intake.select :open @13:27:34.19 Intake @15.2psia, vacuum from 9.7 to 12.7psi @13:27:34.21 SS.reconfigure :inhaling 2.20ml/s @13:27:34.25 SS.seek 25ml @13:27:44.13 Vacuum too high -- waiting up to 40 seconds @13:27:44.14 SS.reconfigure :exhale @13:27:44.18 SS.hold @13:27:49.11 Intake @13.5psia, vacuum from 8.0 to 11.0psi @13:27:49.13 SS.reconfigure :inhaling 1.98ml/s @13:27:49.17 SS.seek 25ml @13:27:52.76 Vacuum too high -- waiting up to 40 seconds @13:27:52.77 SS.reconfigure :exhale @13:27:52.81 SS.hold @13:28:00.55 Intake @14.2psia, vacuum from 8.7 to 11.7psi @13:28:00.56 SS.reconfigure :inhaling 1.79ml/s @13:28:00.61 SS.seek 25ml @13:28:04.63 Stroke completed -- waiting for pressure to equalize ... @13:28:04.64 SS.reconfigure :exhale @13:28:04.68 SS.hold @13:28:13.22 Intake.select :close @13:28:13.35 SSV.dial :exhaust @13:28:14.11 Exhaust.select :open @13:28:14.21 SS.seek 0ml @13:28:23.17 Exhaust.select :close @13:28:23.27 SSV.dial CBV @13:28:23.29
CBV.dial SSV @13:28:24.06 Allowing 130s for 25.0ml of remaining 975.0ml @13:28:24.07 Intake.select :open @13:28:26.22 Intake @15.0psia, vacuum from 9.5 to 12.5psi @13:28:26.24 SS.reconfigure :inhaling 1.79ml/s @13:28:26.28 SS.seek 25ml @13:28:33.29 Vacuum too high -- waiting up to 40 seconds @13:28:33.30 SS.reconfigure :exhale @13:28:33.34 SS.hold @13:28:40.98 Intake @14.2psia, vacuum from 8.7 to 11.7psi @13:28:40.99 SS.reconfigure :inhaling 1.61ml/s @13:28:41.03 SS.seek 25ml @13:28:46.38 Vacuum too high -- waiting up to 40 seconds @13:28:46.39 SS.reconfigure :exhale @13:28:46.43 SS.hold @13:28:56.38 Intake @14.4psia, vacuum from 8.9 to 11.9psi @13:28:56.39 SS.reconfigure :inhaling 1.45ml/s @13:28:56.43 SS.seek 25ml @13:29:02.60 Vacuum too high -- waiting up to 40 seconds @13:29:02.61 SS.reconfigure :exhale @13:29:02.65 SS.hold @13:29:13.86 SS.reconfigure :inhaling 1.30ml/s @13:29:13.90 SS.seek 25ml @13:29:16.03 Stroke completed -- waiting for pressure to equalize ... @13:29:16.04 SS.reconfigure :exhale @13:29:16.08 SS.hold @13:29:23.62 Intake.select :close @13:29:23.76 SSV.dial :exhaust @13:29:24.51 Exhaust.select :open @13:29:24.62 SS.seek 0ml @13:29:33.56 Exhaust.select :close @13:29:33.66 SSV.dial CBV @13:29:33.68
CBV.dial SSV @13:29:34.45 Allowing 130s for 25.0ml of remaining 950.0ml @13:29:34.46 Intake.select :open @13:29:36.61 Intake @15.1psia, vacuum from 9.6 to 12.6psi @13:29:36.63 SS.reconfigure :inhaling 1.30ml/s @13:29:36.67 SS.seek 25ml @13:29:45.63 Vacuum too high -- waiting up to 40 seconds @13:29:45.64 SS.reconfigure :exhale @13:29:45.68 SS.hold @13:29:54.28 Intake @14.3psia, vacuum from 8.8 to 11.8psi @13:29:54.30 SS.reconfigure :inhaling 1.17ml/s @13:29:54.34 SS.seek 25ml @13:30:00.06 Vacuum too high -- waiting up to 40 seconds @13:30:00.07 SS.reconfigure :exhale @13:30:00.12 SS.hold @13:30:11.30 Intake @14.5psia, vacuum from 9.0 to 12.0psi @13:30:11.31 SS.reconfigure :inhaling 1.06ml/s @13:30:11.35 SS.seek 25ml @13:30:20.10 Vacuum too high -- waiting up to 40 seconds @13:30:20.11 SS.reconfigure :exhale @13:30:20.15 SS.hold @13:30:34.48 Intake @14.6psia, vacuum from 9.1 to 12.1psi @13:30:34.50 SS.reconfigure :inhaling 0.95ml/s @13:30:34.54 SS.seek 25ml @13:30:36.28 Stroke completed -- waiting for pressure to equalize ... @13:30:36.29 SS.reconfigure :exhale @13:30:36.33 SS.hold @13:30:44.76 Intake.select :close @13:30:44.90 SSV.dial :exhaust @13:30:45.64 Exhaust.select :open @13:30:45.74 SS.seek 0ml @13:30:54.46 Exhaust.select :close @13:30:54.57 SSV.dial CBV @13:30:54.62
CBV.dial SSV @13:30:55.32 Allowing 130s for 25.0ml of remaining 925.0ml @13:30:55.33 Intake.select :open @13:30:57.49 Intake @15.1psia, vacuum from 9.6 to 12.6psi @13:30:57.50 SS.reconfigure :inhaling 0.95ml/s @13:30:57.55 SS.seek 25ml @13:31:10.09 Vacuum too high -- waiting up to 40 seconds @13:31:10.10 SS.reconfigure :exhale @13:31:10.14 SS.hold @13:31:20.40 Intake @14.6psia, vacuum from 9.1 to 12.1psi @13:31:20.42 SS.reconfigure :inhaling 0.87ml/s @13:31:20.46 SS.seek 25ml @13:31:29.88 Vacuum too high -- waiting up to 40 seconds @13:31:29.89 SS.reconfigure :exhale @13:31:29.93 SS.hold @13:31:42.56 SS.reconfigure :inhaling 0.78ml/s @13:31:42.60 SS.seek 25ml @13:31:53.40 Stroke completed -- waiting for pressure to equalize ... @13:31:53.41 SS.reconfigure :exhale @13:31:53.45 SS.hold @13:32:15.75 Intake.select :close @13:32:15.88 SSV.dial :exhaust @13:32:16.62 Exhaust.select :open @13:32:16.73 SS.seek 0ml @13:32:25.45 Exhaust.select :close @13:32:25.55 SSV.dial CBV @13:32:25.57
CBV.dial SSV @13:32:26.34 Allowing 130s for 25.0ml of remaining 900.0ml @13:32:26.35 Intake.select :open @13:32:28.50 Intake @15.0psia, vacuum from 9.5 to 12.5psi @13:32:28.52 SS.reconfigure :inhaling 0.78ml/s @13:32:28.56 SS.seek 25ml @13:32:43.28 Vacuum too high -- waiting up to 40 seconds @13:32:43.29 SS.reconfigure :exhale @13:32:43.33 SS.hold @13:32:54.49 Intake @14.7psia, vacuum from 9.2 to 12.2psi @13:32:54.51 SS.reconfigure :inhaling 0.70ml/s @13:32:54.55 SS.seek 25ml @13:33:06.22 Vacuum too high -- waiting up to 40 seconds @13:33:06.23 SS.reconfigure :exhale @13:33:06.27 SS.hold @13:33:20.60 Intake @14.8psia, vacuum from 9.3 to 12.3psi @13:33:20.61 SS.reconfigure :inhaling 0.63ml/s @13:33:20.66 SS.seek 25ml @13:33:33.13 Stroke completed -- waiting for pressure to equalize ... @13:33:33.14 SS.reconfigure :exhale @13:33:33.18 SS.hold @13:33:55.59 Intake.select :close @13:33:55.72 SSV.dial :exhaust @13:33:56.48 Exhaust.select :open @13:33:56.58 SS.seek 0ml @13:34:05.55 Exhaust.select :close @13:34:05.66 SSV.dial CBV @13:34:05.69
CBV.dial SSV @13:34:06.43 Allowing 130s for 25.0ml of remaining 875.0ml @13:34:06.44 Intake.select :open @13:34:08.59 Intake @15.1psia, vacuum from 9.6 to 12.6psi @13:34:08.61 SS.reconfigure :inhaling 0.63ml/s @13:34:08.65 SS.seek 25ml @13:34:27.12 Vacuum too high -- waiting up to 40 seconds @13:34:27.13 SS.reconfigure :exhale @13:34:27.17 SS.hold @13:34:39.80 Intake @14.7psia, vacuum from 9.2 to 12.2psi @13:34:39.82 SS.reconfigure :inhaling 0.57ml/s @13:34:39.87 SS.seek 25ml @13:34:56.56 Vacuum too high -- waiting up to 40 seconds @13:34:56.57 SS.reconfigure :exhale @13:34:56.61 SS.hold @13:35:12.77 Intake @14.8psia, vacuum from 9.3 to 12.3psi @13:35:12.78 SS.reconfigure :inhaling 0.51ml/s @13:35:12.83 SS.seek 25ml @13:35:23.21 Stroke completed -- waiting for pressure to equalize ... @13:35:23.22 SS.reconfigure :exhale @13:35:23.26 SS.hold @13:35:43.50 Intake.select :close @13:35:43.64 SSV.dial :exhaust @13:35:44.40 Exhaust.select :open @13:35:44.51 SS.seek 0ml @13:35:53.33 Exhaust.select :close @13:35:53.43 SSV.dial CBV @13:35:53.46
CBV.dial SSV @13:35:54.23 Allowing 130s for 25.0ml of remaining 850.0ml Intake.select :open @13:35:56.39 Intake @15.1psia, vacuum from 9.6 to 12.6psi @13:35:56.40 SS.reconfigure :inhaling 0.51ml/s @13:35:56.45 SS.seek 25ml @13:36:20.93 Vacuum too high -- waiting up to 40 seconds @13:36:20.94 SS.reconfigure :exhale @13:36:20.98 SS.hold @13:36:34.11 Intake @14.8psia, vacuum from 9.3 to 12.3psi @13:36:34.13 SS.reconfigure :inhaling 0.47ml/s @13:36:34.17 SS.seek 25ml @13:36:56.44 Vacuum too high -- waiting up to 40 seconds @13:36:56.45 SS.reconfigure :exhale @13:36:56.49 SS.hold @13:37:12.75 Intake @14.8psia, vacuum from 9.3 to 12.3psi @13:37:12.77 SS.reconfigure :inhaling 0.43ml/s @13:37:12.81 SS.seek 25ml @13:37:21.24 Stroke completed -- waiting for pressure to equalize ... @13:37:21.25 SS.reconfigure :exhale @13:37:21.29 SS.hold @13:37:39.51 Intake.select :close @13:37:39.64 SSV.dial :exhaust @13:37:40.39 Exhaust.select :open @13:37:40.50 SS.seek 0ml @13:37:49.30 Exhaust.select :close @13:37:49.41 SSV.dial CBV @13:37:49.45
CBV.dial SSV @13:37:50.18 Allowing 130s for 25.0ml of remaining 825.0ml @13:37:50.19 Intake.select :open @13:37:52.34 Intake @15.1psia, vacuum from 9.6 to 12.6psi @13:37:52.35 SS.reconfigure :inhaling 0.43ml/s @13:37:52.40 SS.seek 25ml @13:38:24.05 Vacuum too high -- waiting up to 40 seconds @13:38:24.06 SS.reconfigure :exhale @13:38:24.10 SS.hold @13:38:36.78 Intake @14.7psia, vacuum from 9.2 to 12.2psi @13:38:36.79 SS.reconfigure :inhaling 0.38ml/s @13:38:36.84 SS.seek 25ml @13:39:10.93 Vacuum too high -- waiting up to 40 seconds @13:39:10.94 SS.reconfigure :exhale @13:39:10.98 SS.hold @13:39:29.18 Intake @14.8psia, vacuum from 9.3 to 12.3psi @13:39:29.20 SS.reconfigure :inhaling 0.34ml/s @13:39:29.24 SS.seek 25ml @13:39:30.05 Stroke completed -- waiting for pressure to equalize ... @13:39:30.06 SS.reconfigure :exhale @13:39:30.10 SS.hold @13:39:42.00 Intake.select :close @13:39:42.14 SSV.dial :exhaust @13:39:42.89 Exhaust.select :open @13:39:43.00 SS.seek 0ml @13:39:51.75 Exhaust.select :close @13:39:51.86 SSV.dial CBV @13:39:51.87
CBV.dial SSV @13:39:52.64 Allowing 130s for 25.0ml of remaining 800.0ml @13:39:52.65 Intake.select :open @13:39:54.81 Intake @15.1psia, vacuum from 9.6 to 12.6psi @13:39:54.83 SS.reconfigure :inhaling 0.34ml/s @13:39:54.87 SS.seek 25ml @13:41:06.86 Vacuum too high -- waiting up to 40 seconds @13:41:06.87 SS.reconfigure :exhale @13:41:06.92 SS.hold @13:41:25.14 Intake @14.8psia, vacuum from 9.3 to 12.3psi @13:41:25.15 SS.reconfigure :inhaling 0.31ml/s @13:41:25.20 SS.seek 25ml @13:41:35.11 Stroke completed -- waiting for pressure to equalize ... @13:41:35.12 SS.reconfigure :exhale @13:41:35.16 SS.hold @13:41:53.49 Intake.select :close @13:41:53.63 SSV.dial :exhaust @13:41:54.39 Exhaust.select :open @13:41:54.50 SS.seek 0ml @13:42:03.51 Exhaust.select :close @13:42:03.62 SSV.dial CBV @13:42:03.65
CBV.dial SSV @13:42:04.41 Allowing 130s for 25.0ml of remaining 775.0ml @13:42:04.42 Intake.select :open @13:42:06.57 Intake @15.1psia, vacuum from 9.6 to 12.6psi @13:42:06.59 SS.reconfigure :inhaling 0.31ml/s @13:42:06.63 SS.seek 25ml @13:43:35.62 Stroke completed -- waiting for pressure to equalize ... @13:43:35.63 SS.reconfigure :exhale @13:43:35.67 SS.hold @13:44:10.26 Intake.select :close @13:44:10.40 SSV.dial :exhaust @13:44:11.14 Exhaust.select :open @13:44:11.24 SS.seek 0ml @13:44:20.04 Exhaust.select :close @13:44:20.14 SSV.dial CBV @13:44:20.18
CBV.dial SSV @13:44:20.92 Allowing 130s for 25.0ml of remaining 750.0ml @13:44:20.93 Intake.select :open @13:44:23.08 Intake @15.0psia, vacuum from 9.5 to 12.5psi @13:44:23.09 SS.reconfigure :inhaling 0.31ml/s @13:44:23.14 SS.seek 25ml @13:45:47.39 Vacuum too high -- waiting up to 40 seconds @13:45:47.40 SS.reconfigure :exhale @13:45:47.44 SS.hold @13:46:08.28 Intake @14.8psia, vacuum from 9.3 to 12.3psi @13:46:08.29 SS.reconfigure :inhaling 0.28ml/s @13:46:08.34 SS.seek 25ml @13:46:14.76 Stroke completed -- waiting for pressure to equalize ... @13:46:14.77 SS.reconfigure :exhale @13:46:14.81 SS.hold @13:46:30.95 SS.reconfigure :default @13:46:30.99 SS.hold @13:46:31.09 Intake.select :close @13:46:31.23 ShallowSampler::Clogged in MAIN -- Filtering too Slowly after 275.0ml @13:46:31.24 SSV.dial :exhaust @13:46:31.99 Exhaust.select :open @13:46:32.10 SS.reconfigure :exhale @13:46:32.14 SS.seek 0ml @13:46:40.96 Exhaust.select :close @13:46:41.06 Sampled 275.0ml @13:46:41.08 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) Intake.select :close @13:46:41.14 CSV.dial :waste @13:46:41.21 CS.seek 0ml @13:46:41.28 CSV.dial :air @13:46:41.34 SSV.dial CBV,avoiding: :exhaust @13:46:41.35
CBV.dial SSV @13:46:42.04 CS.seek 10ml @13:46:43.21
CTV.dial :air @13:46:44.00 delay 15 seconds @13:46:59.02 SSV.dial :spit,avoiding: :exhaust @13:47:00.35 CTV.dial CSV,avoiding: :intake @13:47:00.37
SS.seek 0ml @13:47:00.49 SSV.dial CBV,avoiding: :exhaust @13:47:06.63 CBV.dial between SSV and waste2 @13:47:07.02 CTV.dial CSV,avoiding: :air @13:47:07.04
CSV.dial CTV @13:47:07.49 CS.reconfigure :pressurize @13:47:07.53 CS.seek 0ml @13:47:26.08 BubblePoint: Intake @39.0psia, Outlet @14.8psia @13:47:26.09 CBV.dial SSV @13:47:26.41 SS.reconfigure :default @13:47:26.45 SS.seek 10ml @13:47:32.05 delay 15 seconds @13:47:47.17 Evacuated: Intake @16.4psia, Outlet @9.3psia @13:47:47.18 SSV.dial :spit,avoiding: :exhaust @13:47:48.50 SS.reconfigure :exhale @13:47:48.54 SS.seek 0ml @13:47:54.00 SS.reconfigure :fast @13:47:54.04 SS.seek 5ml @13:47:56.78 SSV.dial CBV,avoiding: :exhaust @13:47:56.80
CBV.dial SSV @13:47:58.14 CTV.dial :air,avoiding: :intake @13:47:59.49 CSV.dial CTV @13:47:59.52
SS.seek 10ml @13:48:00.51 CSV.dial :air @13:48:00.96 CS.reconfigure :fast @13:48:01.00 CS.seek 0ml @13:48:01.96
SSV.dial :spit,avoiding: :exhaust @13:48:03.28 SS.reconfigure :exhale @13:48:03.33 SS.seek 0ml @13:48:08.38 SS.reconfigure :fast @13:48:08.42 SS.seek 5ml @13:48:08.57 CS.stop @13:48:11.25 SSV.dial CBV,avoiding: :exhaust @13:48:11.28
CBV.dial SSV @13:48:13.58 SSV.dial :spit,avoiding: :exhaust @13:48:14.89 SS.reconfigure :default @13:48:14.93 SS.seek 0ml @13:48:18.08 SS.stop @13:48:18.12 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @13:48:18.13 Intake.select :close @13:48:18.20 CSV.dial :waste @13:48:18.79 SSV.dial CBV,avoiding: :exhaust @13:48:18.80
CBV.dial SSV @13:48:18.96 CS.seek 0ml @13:48:19.02 CSV.dial :air @13:48:19.76 CS.seek 10ml @13:48:20.12
CTV.dial :air @13:48:20.13 delay 15 seconds @13:48:35.15 SSV.dial :spit,avoiding: :exhaust @13:48:36.47 CTV.dial CSV,avoiding: :intake @13:48:36.51
SS.reconfigure :exhale @13:48:36.58 SS.seek 0ml @13:48:37.01 SSV.dial CBV,avoiding: :exhaust @13:48:44.01 CBV.dial between SSV and waste2 @13:48:44.39 CTV.dial CSV,avoiding: :air @13:48:44.41
CSV.dial CTV @13:48:44.87 CS.reconfigure :pressurize @13:48:44.91 CS.seek 0ml @13:49:06.27 BubblePoint: Intake @37.9psia, Outlet @13.7psia @13:49:06.28 CBV.dial SSV @13:49:06.60 SS.reconfigure :default @13:49:06.64 SS.seek 10ml @13:49:12.19 delay 15 seconds @13:49:27.31 Evacuated: Intake @17.9psia, Outlet @11.3psia @13:49:27.32 SSV.dial :spit,avoiding: :exhaust @13:49:28.65 SS.reconfigure :exhale @13:49:28.69 SS.seek 0ml @13:49:33.72 SS.reconfigure :fast @13:49:33.76 SS.seek 5ml @13:49:36.72 SSV.dial CBV,avoiding: :exhaust @13:49:36.73
CBV.dial SSV @13:49:38.07 CTV.dial :air,avoiding: :intake @13:49:39.41 CSV.dial CTV @13:49:39.44
SS.seek 10ml @13:49:40.43 CSV.dial :air @13:49:40.88 CS.reconfigure :fast @13:49:40.92 CS.seek 0ml @13:49:42.37
SSV.dial :spit,avoiding: :exhaust @13:49:43.70 SS.reconfigure :exhale @13:49:43.74 SS.seek 0ml @13:49:46.01 CS.stop @13:49:48.59
SS.reconfigure :fast @13:49:48.63 SS.seek 5ml @13:49:51.51 SSV.dial CBV,avoiding: :exhaust @13:49:51.53
CBV.dial SSV @13:49:53.87 SSV.dial :spit,avoiding: :exhaust @13:49:55.18 SS.reconfigure :default @13:49:55.23 SS.seek 0ml @13:49:58.47 SS.stop @13:49:58.51 Lysing: Adding 2.0ml AqMeOH @13:49:58.52 CBV.dial between CSV and waste1,avoiding: :DBV @13:50:00.02 CTV.dial CSV @13:50:01.34 CSV.dial CSR @13:50:02.13 Pulling 1ml of CSR.AqMeOH @13:50:02.14 CSR.select :AqMeOH @13:50:02.25 CS.reconfigure :default @13:50:02.29 CS.seek 1ml @13:50:05.70 delay 5 seconds @13:50:10.72 CSR.select :air @13:50:10.87 CS.seek 6ml @13:50:24.48 delay 2 seconds @13:50:26.50 CSV.dial :waste2 @13:50:26.92 CS.reconfigure :fast @13:50:26.97 CS.seek 0ml @13:50:41.36 CSV.dial CSR @13:50:41.86 Pulling 2ml of CSR.AqMeOH @13:50:41.87 CSR.select :AqMeOH @13:50:41.98 CS.reconfigure :default @13:50:42.02 CS.seek 2ml @13:50:47.89 delay 5 seconds @13:50:52.91 CSR.select :air @13:50:53.06 CS.seek 5ml @13:51:01.73 delay 2 seconds @13:51:03.75 CSV.dial CTV @13:51:04.17 CS.seek 1ml @13:51:15.30 Before lysing Collection Puck Heater at 14.8C/38.7psia @13:51:15.32 Heating Puck.from 2, :sh1 in Collection Clamp to 70C for 10:00 @13:51:15.34 CH.seek 70C,8:00 @13:53:19.53 CH.hold 10:00,70C @13:53:19.71 Begun lysing Collection Puck Heater at 68.1C/47.3psia @14:03:19.73 Ended lysing Collection Puck Heater at 70C/46.1psia @14:03:19.75 CS.seek 0ml @14:03:23.38 CTV.dial between spare and air,avoiding: :intake @14:03:24.42 CSV.dial :air @14:03:24.87 CS.seek 5ml @14:03:38.66 CSV.dial CTV @14:03:39.09 delay 2 seconds @14:03:41.11 CSV.dial :air @14:03:41.55 delay 2 seconds @14:03:43.56 CSV.dial CTV @14:03:44.02 delay 2 seconds @14:03:46.03 CSV.dial :air @14:03:46.48 delay 2 seconds @14:03:48.51 CS.seek 0ml @14:04:02.18 Puck @76.6psia after final lysis pressurization @14:04:02.19 Recovering Lysate into Collection syringe @14:04:02.20 CSV.dial CBV @14:04:03.20 CBV.dial CSV @14:04:03.56 CS.seek 5ml @14:04:17.22 delay 5 seconds @14:04:22.24 CTV.dial :air @14:04:22.76 CS.seek 7ml @14:04:28.75 delay 5 seconds @14:04:33.77 CSV.dial :air2 @14:04:34.33 CS.seek 10ml @14:04:42.78 CSV.dial between air and CTV,avoiding: CBV @14:04:43.96 Lysing Complete @14:04:44.02 Storing sh1 puck in Collection Clamp to tube 1 @14:04:44.18 SC.reconfigure :tight @14:04:44.22 SC.seek 1 @14:04:47.75 SC.configure :limp @14:04:47.95 Elbow.reconfigure :tight @14:04:47.99 Elbow.seek 1 @14:04:49.79 Forearm.reconfigure :tight @14:04:49.84 Forearm.seek 1 + 450 counts @14:04:50.85 SE.reconfigure :pick @14:04:50.90 SE.seek :beamBreak @14:04:51.90 SE.configure :default @14:04:52.04 Elbow.reconfigure :limp @14:04:52.08 Forearm.reconfigure :loose @14:04:52.12 Forearm.seek 1 @14:04:52.41 Elbow.hold @14:04:52.47 Elbow.reconfigure :tight @14:04:52.56 SE.seek 31.07 Pucks below Top Plate @14:04:53.05 Forearm.force -20 @14:04:53.08 Forearm.seek 1 + 263 counts @14:04:53.36 Forearm.reconfigure :tight @14:04:53.40 Forearm.seek 1 + 450 counts @14:04:53.69 SE.seek 32.07 Pucks below Top Plate @14:04:54.23 Forearm.reconfigure :loose @14:04:54.27 Forearm.seek :retract @14:04:55.68 Elbow.seek CC @14:04:57.42 Elbow.reconfigure :loose @14:04:57.51 Forearm.seek CC @14:04:58.49 Hand.close @14:04:59.06 CC.seek :open @14:05:03.78 Forearm.seek :retract @14:05:04.78 Elbow.reconfigure :tight @14:05:04.82 Elbow.seek 1 @14:05:06.55 Elbow.reconfigure :limp @14:05:06.60 Forearm.seek 1 @14:05:07.59 Elbow.hold @14:05:07.65 Elbow.reconfigure :tight @14:05:07.69 Hand.open @14:05:08.42 Forearm.force -20 @14:05:08.45 Forearm.seek 1 + 312 counts @14:05:08.74 Forearm.reconfigure :tight @14:05:08.79 Forearm.seek 1 + 450 counts @14:05:09.03 SE.seek :down @14:05:09.56 SE.stop @14:05:10.15
Forearm.reconfigure :loose @14:05:10.19 Forearm.seek :retract @14:05:11.12 Tranferring DA @14:05:11.13 Beginning samplePassC @14:05:11.15 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:05:11.32 Elbow.seek FlushPuck::Garage @14:05:13.13 Elbow.reconfigure :loose @14:05:13.21 Forearm.seek :garage @14:05:14.17 Hand.close @14:05:14.92 Forearm.seek :retract @14:05:15.89 Elbow.reconfigure :tight @14:05:15.93 Elbow.seek CC @14:05:18.88 Elbow.reconfigure :loose @14:05:18.96 Forearm.reconfigure :tight @14:05:19.01 Forearm.seek CC @14:05:20.17 CC.seek :closed @14:05:24.68 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.33 @14:05:24.74 Hand.open @14:05:25.36 Forearm.reconfigure :loose @14:05:25.41 Forearm.seek :retract @14:05:26.41 PSV.dial PSR @14:05:27.81 Pulling 1.5ml of PSR.flush @14:05:27.82 PSR.select :flush @14:05:27.94 PS.seek 1.5ml @14:05:44.04 delay 5 seconds @14:05:49.06 PSV.dial :waste1 @14:05:49.51 PS.seek 0ml @14:06:05.60 PSV.dial PSR @14:06:06.05 PSR.select :air @14:06:06.16 PS.seek 2.5ml @14:06:32.54 PRV.dial CBV,avoiding: :ab2 @14:06:32.56
CBV.dial PRV @14:06:34.26 PSV.dial :mixloop @14:06:35.30 CTV.dial :air,avoiding: :intake @14:06:35.32 PTV.dial :PRVmixing @14:06:36.64 PS.seek 0ml @14:07:02.91 PSV.dial :air @14:07:03.48 PS.seek 2.5ml @14:07:29.78 PSV.dial :mixloop @14:07:30.23 PS.seek 0ml @14:07:56.53 PSV.dial :air @14:07:57.00 PS.seek 2.5ml @14:08:23.41 PSV.dial :mixloop @14:08:23.87 PS.seek 0ml @14:08:50.25 Garaging FlushPuck in Collection Clamp @14:08:50.80 Elbow.reconfigure :tight @14:08:50.84 Elbow.seek CC @14:08:51.20 Elbow.reconfigure :loose @14:08:51.29 Forearm.seek CC @14:08:52.29 Hand.close @14:08:52.93 CC.seek :open @14:08:57.54 Forearm.seek :retract @14:08:58.54 Elbow.reconfigure :tight @14:08:58.59 Elbow.seek :garage @14:09:01.61 Elbow.reconfigure :loose @14:09:01.70 Forearm.reconfigure :tight @14:09:01.75 Forearm.seek :garage @14:09:02.78 Hand.open @14:09:03.49 Forearm.reconfigure :loose @14:09:03.53 Forearm.seek :retract @14:09:04.51 PSV.dial :mixloop @14:09:04.52 PTV.dial :PRVmixing @14:09:04.53 PRV.dial CBV @14:09:04.55
CBV.dial PRV @14:09:04.57 CTV.dial :air,avoiding: :intake @14:09:04.59 Loading lyfil puck from tube 3 into Collection Clamp @14:09:04.81 SC.reconfigure :tight @14:09:04.85 SC.seek 3 @14:09:11.59 SC.configure :limp @14:09:11.78 Elbow.reconfigure :tight @14:09:11.82 Elbow.seek 3 @14:09:13.77 Forearm.reconfigure :tight @14:09:13.82 Forearm.seek 3 + 450 counts @14:09:14.83 SE.reconfigure :pick @14:09:14.88 SE.seek :beamBreak @14:09:15.82 SE.configure :default @14:09:15.96 Elbow.seek 3 @14:09:16.13 Forearm.seek 3 + 300 counts @14:09:16.32 Forearm.reconfigure :loose @14:09:16.36 Hand.close @14:09:16.95 SE.seek :down @14:09:17.13
Forearm.seek :retract @14:09:17.56 SE.stop @14:09:18.14
Elbow.seek CC @14:09:19.78 Elbow.reconfigure :loose @14:09:19.87 Forearm.reconfigure :tight @14:09:19.91 Forearm.seek CC @14:09:21.15 CC.seek :closed @14:09:25.67 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.09 @14:09:25.68 Hand.open @14:09:26.36 Forearm.reconfigure :loose @14:09:26.40 Forearm.seek :retract @14:09:27.35 CTV.dial CSV,avoiding: :intake @14:09:28.70 CSV.dial CTV @14:09:29.00 PS.seek 2.5ml @14:09:55.43 CS.seek 4.5ml @14:10:10.65 delay 8 seconds @14:10:18.67 PSV.dial :air @14:10:19.13 delay 2 seconds @14:10:21.14 PSV.dial :waste2 @14:10:21.88 PS.seek 0ml @14:10:48.25 CBV.dial between PRV and waste1 @14:10:48.59 CSV.dial :air @14:10:49.05 CS.seek 10ml @14:11:03.90 CSV.dial CTV @14:11:04.34 CS.seek 0ml @14:11:30.68 CBV.dial :waste1 @14:11:30.98 delay 5 seconds @14:11:36.00 CTV.dial :air,avoiding: :intake @14:11:37.85 Storing lyfil puck in Collection Clamp to tube 2 @14:11:38.01 SC.reconfigure :tight @14:11:38.05 SC.seek 2 @14:11:41.65 SC.configure :limp @14:11:41.80 SE.reconfigure :place @14:11:41.84 SE.seek :beamBreak @14:11:41.90
Elbow.reconfigure :tight @14:11:41.95 Elbow.seek CC @14:11:42.22 Elbow.reconfigure :loose @14:11:42.30 Forearm.seek CC @14:11:43.27 Hand.close @14:11:43.93 CC.seek :open @14:11:44.69 SE.configure :default @14:11:44.74 SE.seek :place @14:11:48.64
Forearm.seek :retract @14:11:49.59 Elbow.reconfigure :tight @14:11:49.63 Elbow.seek 2 @14:11:51.34 Elbow.reconfigure :limp @14:11:51.39 Forearm.seek 2 @14:11:52.36 Elbow.hold @14:11:52.43 Elbow.reconfigure :tight @14:11:52.47 Hand.open @14:11:53.16 Forearm.force -20 @14:11:53.20 Forearm.seek 2 + 293 counts @14:11:53.48 Forearm.reconfigure :tight @14:11:53.53 Forearm.seek 2 + 450 counts @14:11:53.90 SE.seek :down @14:11:55.02
Forearm.reconfigure :loose @14:11:55.06 Forearm.seek :retract @14:11:56.57 SE.stop @14:11:56.61
PSV.dial :air @14:11:57.35 PS.seek 2.5ml @14:12:23.66 delay 1 second @14:12:24.67 PSV.dial :mixloop @14:12:25.11 delay 1 second @14:12:26.12 PSV.dial :air @14:12:26.58 delay 1 second @14:12:27.59 PSV.dial :mixloop @14:12:28.08 delay 1 second @14:12:29.09 PSV.dial :air @14:12:29.58 delay 1 second @14:12:30.60 PS.seek 0ml @14:12:56.97 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @14:12:57.14 Elbow.seek FlushPuck::Garage @14:12:58.93 Elbow.reconfigure :loose @14:12:59.02 Forearm.seek :garage @14:13:00.07 Hand.close @14:13:00.78 Forearm.seek :retract @14:13:01.76 Elbow.reconfigure :tight @14:13:01.80 Elbow.seek PC @14:13:03.04 Elbow.reconfigure :loose @14:13:03.13 Forearm.reconfigure :tight @14:13:03.17 Forearm.seek PC @14:13:04.42 PC.seek :closed @14:13:09.03 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.57 @14:13:09.09 Hand.open @14:13:09.73 Forearm.reconfigure :loose @14:13:09.77 Forearm.seek :retract @14:13:10.70 PRV.dial :air @14:13:11.16 PSV.dial :mixloop @14:13:11.67 PS.seek 0.3ml @14:13:15.56 PSV.dial PSR @14:13:17.13 Pulling 0.4ml of PSR.air @14:13:17.14 PSR.select :air @14:13:17.16 PS.seek 0.7ml @14:13:22.06 delay 3 seconds @14:13:25.08 PSR.select :air @14:13:25.10 PS.seek 2.5ml @14:13:44.40 delay 3 seconds @14:13:47.42 PSV.dial :mixloop @14:13:48.84 PTV.dial :mixing @14:13:49.61 PBV.dial :waste2 @14:13:50.57 PS.seek 0ml @14:14:17.06 Garaging FlushPuck in Processing Clamp @14:14:17.13 Elbow.reconfigure :tight @14:14:17.17 Elbow.seek PC @14:14:17.28 Elbow.reconfigure :loose @14:14:17.37 Forearm.seek PC @14:14:18.30 Hand.close @14:14:18.93 PC.seek :open @14:14:23.64 Forearm.seek :retract @14:14:24.58 Elbow.reconfigure :tight @14:14:24.63 Elbow.seek :garage @14:14:25.98 Elbow.reconfigure :loose @14:14:26.07 Forearm.reconfigure :tight @14:14:26.11 Forearm.seek :garage @14:14:27.13 Hand.open @14:14:27.73 Forearm.reconfigure :loose @14:14:27.77 Forearm.seek :retract @14:14:28.78 Loading da puck from tube 3 into Processing Clamp @14:14:28.99 SC.reconfigure :tight @14:14:29.03 SC.seek 3 @14:14:32.68 SC.configure :limp @14:14:32.87 Elbow.reconfigure :tight @14:14:32.92 Elbow.seek 3 @14:14:34.78 Forearm.reconfigure :tight @14:14:34.83 Forearm.seek 3 + 450 counts @14:14:35.92 SE.reconfigure :pick @14:14:35.97 SE.seek :beamBreak @14:14:37.01 SE.configure :default @14:14:37.16 Elbow.seek 3 @14:14:37.32 Forearm.seek 3 + 300 counts @14:14:37.50 Forearm.reconfigure :loose @14:14:37.54 Hand.close @14:14:38.19 SE.seek :down @14:14:38.38
Forearm.seek :retract @14:14:38.95 SE.stop @14:14:39.33
Elbow.seek PC @14:14:41.90 Elbow.reconfigure :loose @14:14:41.99 Forearm.reconfigure :tight @14:14:42.03 Forearm.seek PC @14:14:43.24 PC.seek :closed @14:14:47.88 Processing Clamp onSpring/offSpring for 16/26 samples, on/off torque 1.60 @14:14:47.89 Hand.open @14:14:48.61 Forearm.reconfigure :loose @14:14:48.65 Forearm.seek :retract @14:14:49.67 Heating Puck.from 3, :da in Processing Clamp to 28C for 2:00:00 @14:14:49.68 PH.seek 28C,5:00 @14:15:29.54 PH.hold 2:00:00,28C @14:15:29.58 delay 1:30 @14:16:59.62 PSR.select :air @14:17:00.11 PSV.dial PSR @14:17:01.22 Pulling 0.5ml of PSR.wash @14:17:01.23 PSR.select :wash @14:17:01.35 PS.seek 0.5ml @14:17:07.22 delay 5 seconds @14:17:12.23 PSR.select :air @14:17:12.35 PS.seek 2ml @14:17:28.53 PTV.dial :puck @14:17:29.28 PSV.dial PTV @14:17:30.04 PBV.dial :waste1 @14:17:31.45 PS.seek 1.5ml @14:17:37.46 delay 3 seconds @14:17:40.48 PBV.dial between PSV and waste1 @14:17:40.85 PS.seek 0ml @14:17:57.15 delay 5:00 @14:22:57.17 recoverPSRreagent08 @14:22:57.18 PTV.dial :puck @14:22:57.19 PBV.dial :waste1 @14:22:57.55 delay 30 seconds @14:23:27.58 PBV.dial between PSV and waste1 @14:23:27.94 PSR.select :air @14:23:27.96 PSV.dial PSR @14:23:28.73 PS.reconfigure :fast @14:23:28.77 PS.seek 2.5ml @14:23:52.52 PSV.dial PTV @14:23:53.27 delay 5 seconds @14:23:58.29 PSV.dial PSR @14:23:59.05 delay 5 seconds @14:24:04.07 PSV.dial PTV @14:24:04.83 delay 5 seconds @14:24:09.85 PSV.dial :air @14:24:10.73 PS.seek 0ml @14:24:33.83 PTV.dial :PRVmixing,via: PRV @14:24:35.16 PSV.dial :mixloop @14:24:35.62 PRV.dial :ab1 @14:24:36.37 PS.reconfigure :slow @14:24:36.41 PS.seek 0.15ml @14:24:39.96 delay 2 seconds @14:24:41.97 CBV.dial PRV @14:24:42.00
PRV.dial CBV @14:24:42.57 PS.seek 0.35ml @14:24:47.21 delay 2 seconds @14:24:49.22 CBV.dial between PRV and PSV @14:24:49.55 PRV.dial :air @14:24:49.99 PS.seek 0.9ml @14:25:01.67 PRV.dial :waste2 @14:25:02.42 PS.seek 0.5ml @14:25:11.05 addPMLReagent08 @14:25:11.06 PSV.dial :air @14:25:11.65 PS.reconfigure :default @14:25:11.69 PS.seek 2ml @14:25:27.89 PSV.dial :PML @14:25:28.33 PTV.dial :mixing @14:25:29.06 PBV.dial :waste1 @14:25:29.48 PS.seek 1.85ml @14:25:31.83 PBV.dial between PSV and waste1 @14:25:32.21 PS.seek 0ml @14:25:52.08 delay 2 seconds @14:25:54.10 Beginning samplePassC @14:25:54.11 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:25:54.27 Elbow.reconfigure :tight @14:25:54.31 Elbow.seek FlushPuck::Garage @14:25:55.67 Elbow.reconfigure :loose @14:25:55.76 Forearm.seek :garage @14:25:56.74 Hand.close @14:25:57.42 Forearm.seek :retract @14:25:58.36 Elbow.reconfigure :tight @14:25:58.40 Elbow.seek CC @14:26:01.31 Elbow.reconfigure :loose @14:26:01.41 Forearm.reconfigure :tight @14:26:01.45 Forearm.seek CC @14:26:02.69 CC.seek :closed @14:26:07.20 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.31 @14:26:07.27 Hand.open @14:26:07.97 Forearm.reconfigure :loose @14:26:08.02 Forearm.seek :retract @14:26:09.46 PSV.dial PSR @14:26:10.55 Pulling 1.5ml of PSR.flush @14:26:10.56 PSR.select :flush @14:26:10.68 PS.seek 1.5ml @14:26:26.89 delay 5 seconds @14:26:31.91 PSV.dial :waste1 @14:26:32.37 PS.seek 0ml @14:26:48.52 PSV.dial PSR @14:26:48.98 PSR.select :air @14:26:49.10 PS.seek 2.5ml @14:27:15.40 PRV.dial CBV,avoiding: :ab2 @14:27:15.42
CBV.dial PRV @14:27:17.05 PSV.dial :mixloop @14:27:18.09 CTV.dial :air,avoiding: :intake @14:27:18.11 PTV.dial :PRVmixing @14:27:18.87 PS.seek 0ml @14:27:45.23 PSV.dial :air @14:27:45.80 PS.seek 2.5ml @14:28:12.20 PSV.dial :mixloop @14:28:12.65 PS.seek 0ml @14:28:38.98 PSV.dial :air @14:28:39.58 PS.seek 2.5ml @14:29:05.92 PSV.dial :mixloop @14:29:06.36 PS.seek 0ml @14:29:32.67 Garaging FlushPuck in Collection Clamp @14:29:32.74 Elbow.reconfigure :tight @14:29:32.78 Elbow.seek CC @14:29:33.09 Elbow.reconfigure :loose @14:29:33.18 Forearm.seek CC @14:29:34.13 Hand.close @14:29:34.79 CC.seek :open @14:29:39.43 Forearm.seek :retract @14:29:40.38 Elbow.reconfigure :tight @14:29:40.43 Elbow.seek :garage @14:29:43.44 Elbow.reconfigure :loose @14:29:43.53 Forearm.reconfigure :tight @14:29:43.57 Forearm.seek :garage @14:29:44.58 Hand.open @14:29:45.22 Forearm.reconfigure :loose @14:29:45.26 Forearm.seek :retract @14:29:46.26 delayUntil "14:33:54.09" @14:29:46.29 Whole Cell Archive sampling 1000ml @14:29:46.30 Loading wcr puck from tube 3 into Collection Clamp @14:29:46.53 SC.reconfigure :tight @14:29:46.57 SC.seek 3 @14:29:46.63 SC.configure :limp @14:29:46.84 Elbow.reconfigure :tight @14:29:46.88 Elbow.seek 3 @14:29:48.86 Forearm.reconfigure :tight @14:29:48.90 Forearm.seek 3 + 450 counts @14:29:49.91 SE.reconfigure :pick @14:29:49.97 SE.seek :beamBreak @14:29:51.19 SE.configure :default @14:29:51.34 Elbow.seek 3 @14:29:51.51 Forearm.seek 3 + 300 counts @14:29:51.69 Forearm.reconfigure :loose @14:29:51.74 Hand.close @14:29:52.43 SE.seek :down @14:29:52.62 Forearm.seek :retract @14:29:53.19 SE.stop @14:29:53.57 Elbow.seek CC @14:29:55.30 Elbow.reconfigure :loose @14:29:55.40 Forearm.reconfigure :tight @14:29:55.44 Forearm.seek CC @14:29:56.67 CC.seek :closed @14:30:01.19 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.21 @14:30:01.20 Hand.open @14:30:01.85 Forearm.reconfigure :loose @14:30:01.90 Forearm.seek :retract @14:30:02.92 collectSample 1000ml w/bubble point of 35psi @14:30:02.93 Priming 7ml @14:30:02.94 Intake.select :close @14:30:02.95 Exhaust.select :close @14:30:02.96 SS.hold @14:30:03.15 Calibrating pressure sensors @14:30:03.17 CTV.dial :air @14:30:03.18 SSV.dial :spit,avoiding: :exhaust @14:30:03.20 SS.reconfigure :fast @14:30:03.24 SS.seek 5ml @14:30:05.92 SSV.dial CBV @14:30:05.96 CBV.dial SSV @14:30:07.27 delay 3.8 seconds @14:30:11.20 SSV.dial :spit @14:30:12.51 SS.seek 0ml @14:30:15.24 SSV.dial CBV @14:30:16.57 CTV.dial :intake @14:30:17.34 SSV.dial CBV @14:30:17.36 CBV.dial SSV @14:30:17.38 Allowing 40s for 7.0ml of remaining 7.0ml @14:30:17.39 Intake.select :open @14:30:19.54 Intake @15.3psia, vacuum from 9.8 to 12.8psi @14:30:19.56 SS.reconfigure :inhaling 2.20ml/s @14:30:19.60 SS.seek 7ml @14:30:26.55 Stroke completed -- waiting for pressure to equalize ... @14:30:26.56 SS.reconfigure :exhale @14:30:26.61 SS.hold @14:30:36.29 Intake.select :close @14:30:36.43 SSV.dial :exhaust @14:30:37.18 Exhaust.select :open @14:30:37.29 SS.seek 0ml @14:30:41.41 Exhaust.select :close @14:30:41.52 Sampling 1000.0ml @14:30:41.53 Intake.select :close @14:30:41.54 Exhaust.select :close @14:30:41.55 SS.reconfigure :default @14:30:41.59 SS.hold @14:30:41.90 CTV.dial :intake @14:30:41.94 SSV.dial CBV @14:30:42.02 CBV.dial SSV @14:30:42.78 Allowing 130s for 25.0ml of remaining 1000.0ml @14:30:42.79 Intake.select :open @14:30:44.94 Intake @16.3psia, vacuum from 10.0 to 13.0psi @14:30:44.96 SS.reconfigure :inhaling 2.20ml/s @14:30:45.00 SS.seek 25ml @14:30:50.62 Vacuum too high -- waiting up to 40 seconds @14:30:50.63 SS.reconfigure :exhale @14:30:50.68 SS.hold @14:30:53.02 Intake @14.9psia, vacuum from 9.4 to 12.4psi @14:30:53.04 SS.reconfigure :inhaling 1.98ml/s @14:30:53.08 SS.seek 25ml @14:31:01.75 Vacuum too high -- waiting up to 40 seconds @14:31:01.76 SS.reconfigure :exhale @14:31:01.80 SS.hold @14:31:07.70 Intake @14.4psia, vacuum from 8.9 to 11.9psi @14:31:08.20 SS.reconfigure :inhaling 1.79ml/s @14:31:08.25 SS.seek 25ml @14:31:10.96 Stroke completed -- waiting for pressure to equalize ... @14:31:10.97 SS.reconfigure :exhale @14:31:11.01 SS.hold @14:31:13.92 Intake.select :close @14:31:14.06 SSV.dial :exhaust @14:31:14.82 Exhaust.select :open @14:31:14.93 SS.seek 0ml @14:31:23.74 Exhaust.select :close @14:31:23.85 SSV.dial CBV @14:31:23.88 CBV.dial SSV @14:31:24.63 Allowing 130s for 25.0ml of remaining 975.0ml @14:31:24.64 Intake.select :open @14:31:26.80 Intake @16.1psia, vacuum from 10.0 to 13.0psi @14:31:26.82 SS.reconfigure :inhaling 1.79ml/s @14:31:26.86 SS.seek 25ml @14:31:35.62 Vacuum too high -- waiting up to 40 seconds @14:31:35.63 SS.reconfigure :exhale @14:31:35.68 SS.hold @14:31:37.17 Intake @13.6psia, vacuum from 8.1 to 11.1psi @14:31:37.19 SS.reconfigure :inhaling 1.61ml/s @14:31:37.23 SS.seek 25ml @14:31:46.88 Stroke completed -- waiting for pressure to equalize ... @14:31:46.89 SS.reconfigure :exhale @14:31:46.93 SS.hold @14:31:54.32 Intake.select :close @14:31:54.46 SSV.dial :exhaust @14:31:55.22 Exhaust.select :open @14:31:55.33 SS.seek 0ml @14:32:04.06 Exhaust.select :close @14:32:04.17 SSV.dial CBV @14:32:04.20 CBV.dial SSV @14:32:04.96 Allowing 130s for 25.0ml of remaining 950.0ml @14:32:04.97 Intake.select :open @14:32:07.13 Intake @15.9psia, vacuum from 10.0 to 13.0psi @14:32:07.15 SS.reconfigure :inhaling 1.61ml/s @14:32:07.19 SS.seek 25ml @14:32:20.12 Vacuum too high -- waiting up to 40 seconds @14:32:20.13 SS.reconfigure :exhale @14:32:20.18 SS.hold @14:32:20.98 Intake @13.1psia, vacuum from 7.6 to 10.6psi @14:32:21.00 SS.reconfigure :inhaling 1.45ml/s @14:32:21.04 SS.seek 25ml @14:32:28.11 Stroke completed -- waiting for pressure to equalize ... @14:32:28.12 SS.reconfigure :exhale @14:32:28.17 SS.hold @14:32:34.59 Intake.select :close @14:32:34.74 SSV.dial :exhaust @14:32:35.50 Exhaust.select :open @14:32:35.61 SS.seek 0ml @14:32:44.60 Exhaust.select :close @14:32:44.71 SSV.dial CBV @14:32:44.72 CBV.dial SSV @14:32:45.49 Allowing 130s for 25.0ml of remaining 925.0ml @14:32:45.50 Intake.select :open @14:32:47.66 Intake @15.8psia, vacuum from 10.0 to 13.0psi @14:32:47.67 SS.reconfigure :inhaling 1.45ml/s @14:32:47.72 SS.seek 25ml @14:33:03.34 Vacuum too high -- waiting up to 40 seconds @14:33:03.35 SS.reconfigure :exhale @14:33:03.39 SS.hold @14:33:04.84 Intake @13.3psia, vacuum from 7.8 to 10.8psi @14:33:04.86 SS.reconfigure :inhaling 1.30ml/s @14:33:04.91 SS.seek 25ml @14:33:09.75 Stroke completed -- waiting for pressure to equalize ... @14:33:09.76 SS.reconfigure :exhale @14:33:09.81 SS.hold @14:33:15.35 Intake.select :close @14:33:15.49 SSV.dial :exhaust @14:33:16.25 Exhaust.select :open @14:33:16.36 SS.seek 0ml @14:33:25.11 Exhaust.select :close @14:33:25.22 SSV.dial CBV @14:33:25.24 CBV.dial SSV @14:33:26.00 Allowing 130s for 25.0ml of remaining 900.0ml @14:33:26.02 Intake.select :open @14:33:28.17 Intake @15.7psia, vacuum from 10.0 to 13.0psi @14:33:28.19 SS.reconfigure :inhaling 1.30ml/s @14:33:28.23 SS.seek 25ml @14:33:50.36 Stroke completed -- waiting for pressure to equalize ... @14:33:50.37 SS.reconfigure :exhale @14:33:50.41 SS.hold @14:33:54.09 recoverPRVreagent08 @14:33:54.10 PTV.dial :mixing @14:33:54.87 PBV.dial :waste1 @14:33:55.23 delay 30 seconds @14:33:58.86 Intake.select :close @14:33:59.00 SSV.dial :exhaust @14:33:59.77 Exhaust.select :open @14:33:59.88 SS.seek 0ml @14:34:08.84 Exhaust.select :close @14:34:08.95 SSV.dial CBV @14:34:08.97 CBV.dial SSV @14:34:09.75 Allowing 130s for 25.0ml of remaining 875.0ml @14:34:09.77 Intake.select :open @14:34:11.92 Intake @15.7psia, vacuum from 10.0 to 13.0psi @14:34:11.94 SS.reconfigure :inhaling 1.30ml/s @14:34:11.98 SS.seek 25ml @14:34:25.24 PBV.dial between PSV and waste1 @14:34:25.62 PSV.dial :air @14:34:26.09 PS.reconfigure :fast @14:34:26.14 PS.seek 2.5ml @14:34:30.96 Vacuum too high -- waiting up to 40 seconds @14:34:30.97 SS.reconfigure :exhale @14:34:31.02 SS.hold @14:34:33.59 Intake @13.4psia, vacuum from 7.9 to 10.9psi @14:34:33.61 SS.reconfigure :inhaling 1.17ml/s @14:34:33.65 SS.seek 25ml @14:34:36.87 Stroke completed -- waiting for pressure to equalize ... @14:34:36.89 SS.reconfigure :exhale @14:34:36.93 SS.hold @14:34:42.47 Intake.select :close @14:34:42.61 SSV.dial :exhaust @14:34:43.38 Exhaust.select :open @14:34:43.49 SS.seek 0ml @14:34:50.01 PSV.dial :mixloop @14:34:50.48 delay 2 seconds @14:34:52.49 PSV.dial :air @14:34:52.62 Exhaust.select :close @14:34:52.74 SSV.dial CBV @14:34:52.75 CBV.dial SSV @14:34:53.10 delay 2 seconds @14:34:53.54 Allowing 130s for 25.0ml of remaining 850.0ml @14:34:53.55 Intake.select :open @14:34:55.12 PSV.dial :mixloop @14:34:55.57 delay 2 seconds @14:34:55.70 Intake @15.6psia, vacuum from 10.0 to 13.0psi @14:34:55.72 SS.reconfigure :inhaling 1.17ml/s @14:34:55.77 SS.seek 25ml @14:34:57.59 PSV.dial :air @14:34:58.06 PS.seek 0ml @14:35:19.45 Stroke completed -- waiting for pressure to equalize ... @14:35:19.46 SS.reconfigure :exhale @14:35:19.51 SS.hold @14:35:21.47 washCycleDA #1 of 2 @14:35:21.50 PSR.select :air @14:35:21.54 PSV.dial PSR @14:35:22.93 Pulling 0.5ml of PSR.wash @14:35:22.94 PSR.select :wash @14:35:23.12 PS.reconfigure :default @14:35:23.16 PS.seek 0.5ml @14:35:28.19 Intake.select :close @14:35:28.33 SSV.dial :exhaust @14:35:29.11 Exhaust.select :open @14:35:29.13 delay 5 seconds @14:35:29.25 SS.seek 0ml @14:35:34.15 PSR.select :air @14:35:34.26 PS.seek 2ml @14:35:38.00 Exhaust.select :close @14:35:38.11 SSV.dial CBV @14:35:38.13 CBV.dial SSV @14:35:38.91 Allowing 130s for 25.0ml of remaining 825.0ml @14:35:38.92 Intake.select :open @14:35:41.08 Intake @15.7psia, vacuum from 10.0 to 13.0psi @14:35:41.10 SS.reconfigure :inhaling 1.17ml/s @14:35:41.14 SS.seek 25ml @14:35:50.35 PTV.dial :puck @14:35:51.10 PSV.dial PTV @14:35:51.85 PBV.dial :waste1 @14:35:52.28 PS.seek 1.5ml @14:35:58.29 delay 3 seconds @14:36:01.30 PBV.dial between PSV and waste1 @14:36:01.69 PS.seek 0ml @14:36:03.34 Vacuum too high -- waiting up to 40 seconds @14:36:03.36 SS.reconfigure :exhale @14:36:03.40 SS.hold @14:36:05.91 Intake @13.4psia, vacuum from 7.9 to 10.9psi @14:36:05.93 SS.reconfigure :inhaling 1.06ml/s @14:36:05.97 SS.seek 25ml @14:36:08.47 Stroke completed -- waiting for pressure to equalize ... @14:36:08.48 SS.reconfigure :exhale @14:36:08.52 SS.hold @14:36:14.06 Intake.select :close @14:36:14.20 SSV.dial :exhaust @14:36:14.97 Exhaust.select :open @14:36:15.08 SS.seek 0ml @14:36:17.76 delay 2:00 @14:36:24.09 Exhaust.select :close @14:36:24.20 SSV.dial CBV @14:36:24.22 CBV.dial SSV @14:36:25.00 Allowing 130s for 25.0ml of remaining 800.0ml @14:36:25.01 Intake.select :open @14:36:27.17 Intake @15.5psia, vacuum from 10.0 to 13.0psi @14:36:27.19 SS.reconfigure :inhaling 1.06ml/s @14:36:27.23 SS.seek 25ml @14:36:53.09 Stroke completed -- waiting for pressure to equalize ... @14:36:53.58 SS.reconfigure :exhale @14:36:53.63 SS.hold @14:37:00.95 Intake.select :close @14:37:01.09 SSV.dial :exhaust @14:37:01.86 Exhaust.select :open @14:37:01.97 SS.seek 0ml @14:37:10.68 Exhaust.select :close @14:37:10.79 SSV.dial CBV @14:37:10.82 CBV.dial SSV @14:37:11.58 Allowing 130s for 25.0ml of remaining 775.0ml @14:37:11.59 Intake.select :open @14:37:13.75 Intake @15.6psia, vacuum from 10.0 to 13.0psi @14:37:13.76 SS.reconfigure :inhaling 1.06ml/s @14:37:13.81 SS.seek 25ml @14:37:40.23 Stroke completed -- waiting for pressure to equalize ... @14:37:40.24 SS.reconfigure :exhale @14:37:40.29 SS.hold @14:37:48.73 Intake.select :close @14:37:48.88 SSV.dial :exhaust @14:37:49.64 Exhaust.select :open @14:37:49.75 SS.seek 0ml @14:37:58.57 Exhaust.select :close @14:37:58.68 SSV.dial CBV @14:37:58.70 CBV.dial SSV @14:37:59.48 Allowing 130s for 25.0ml of remaining 750.0ml @14:37:59.50 Intake.select :open @14:38:01.65 Intake @15.6psia, vacuum from 10.0 to 13.0psi @14:38:01.67 SS.reconfigure :inhaling 1.06ml/s @14:38:01.71 SS.seek 25ml @14:38:17.78 recoverPSRreagent08 @14:38:17.79 PTV.dial :puck @14:38:17.80 PBV.dial :waste1 @14:38:18.16 delay 30 seconds @14:38:28.20 Stroke completed -- waiting for pressure to equalize ... @14:38:28.21 SS.reconfigure :exhale @14:38:28.25 SS.hold @14:38:37.91 Intake.select :close @14:38:38.05 SSV.dial :exhaust @14:38:38.81 Exhaust.select :open @14:38:38.93 SS.seek 0ml @14:38:47.68 Exhaust.select :close @14:38:47.79 SSV.dial CBV @14:38:47.81 CBV.dial SSV @14:38:48.18 PBV.dial between PSV and waste1 @14:38:48.55 PSR.select :air @14:38:48.57 PSV.dial PSR @14:38:48.63 Allowing 130s for 25.0ml of remaining 725.0ml @14:38:48.64 Intake.select :open @14:38:49.34 PS.reconfigure :fast @14:38:49.38 PS.seek 2.5ml @14:38:50.79 Intake @15.6psia, vacuum from 10.0 to 13.0psi @14:38:50.81 SS.reconfigure :inhaling 1.06ml/s @14:38:50.86 SS.seek 25ml @14:39:13.25 PSV.dial PTV @14:39:14.02 delay 5 seconds @14:39:14.24 Vacuum too high -- waiting up to 40 seconds @14:39:14.25 SS.reconfigure :exhale @14:39:14.29 SS.hold @14:39:17.83 Intake @13.9psia, vacuum from 8.4 to 11.4psi @14:39:17.85 SS.reconfigure :inhaling 0.95ml/s @14:39:17.89 SS.seek 25ml @14:39:19.04 PSV.dial PSR @14:39:19.80 delay 5 seconds @14:39:21.63 Stroke completed -- waiting for pressure to equalize ... @14:39:21.65 SS.reconfigure :exhale @14:39:21.69 SS.hold @14:39:24.82 PSV.dial PTV @14:39:25.73 delay 5 seconds @14:39:28.11 Intake.select :close @14:39:28.26 SSV.dial :exhaust @14:39:29.03 Exhaust.select :open @14:39:29.14 SS.seek 0ml @14:39:30.75 PSV.dial :air @14:39:31.52 PS.seek 0ml @14:39:38.17 Exhaust.select :close @14:39:38.28 SSV.dial CBV @14:39:38.30 CBV.dial SSV @14:39:39.08 Allowing 130s for 25.0ml of remaining 700.0ml @14:39:39.09 Intake.select :open @14:39:41.24 Intake @15.6psia, vacuum from 10.0 to 13.0psi @14:39:41.26 SS.reconfigure :inhaling 0.95ml/s @14:39:41.30 SS.seek 25ml @14:39:54.85 washCycleDA #2 of 2 @14:39:54.86 PSR.select :air @14:39:54.87 PSV.dial PSR @14:39:56.24 Pulling 0.5ml of PSR.wash @14:39:56.25 PSR.select :wash @14:39:56.37 PS.reconfigure :default @14:39:56.41 PS.seek 0.5ml @14:40:02.35 delay 5 seconds @14:40:07.37 PSR.select :air @14:40:07.48 PS.seek 2ml @14:40:09.68 Stroke completed -- waiting for pressure to equalize ... @14:40:09.69 SS.reconfigure :exhale @14:40:09.74 SS.hold @14:40:19.41 Intake.select :close @14:40:19.56 SSV.dial :exhaust @14:40:20.32 Exhaust.select :open @14:40:20.43 SS.seek 0ml @14:40:23.56 PTV.dial :puck @14:40:23.58 PSV.dial PTV @14:40:24.44 PBV.dial :waste1 @14:40:24.87 PS.seek 1.5ml @14:40:29.24 Exhaust.select :close @14:40:29.34 SSV.dial CBV @14:40:29.36 CBV.dial SSV @14:40:30.14 Allowing 130s for 25.0ml of remaining 675.0ml @14:40:30.15 Intake.select :open @14:40:30.81 delay 3 seconds @14:40:32.30 Intake @15.5psia, vacuum from 10.0 to 13.0psi @14:40:32.32 SS.reconfigure :inhaling 0.95ml/s @14:40:32.36 SS.seek 25ml @14:40:33.83 PBV.dial between PSV and waste1 @14:40:34.21 PS.seek 0ml @14:40:50.35 delay 2:00 @14:41:01.24 Stroke completed -- waiting for pressure to equalize ... @14:41:01.25 SS.reconfigure :exhale @14:41:01.30 SS.hold @14:41:12.33 Intake.select :close @14:41:12.47 SSV.dial :exhaust @14:41:13.23 Exhaust.select :open @14:41:13.35 SS.seek 0ml @14:41:22.34 Exhaust.select :close @14:41:22.44 SSV.dial CBV @14:41:22.47 CBV.dial SSV @14:41:23.23 Allowing 130s for 25.0ml of remaining 650.0ml @14:41:23.24 Intake.select :open @14:41:25.40 Intake @15.7psia, vacuum from 10.0 to 13.0psi @14:41:25.42 SS.reconfigure :inhaling 0.95ml/s @14:41:25.46 SS.seek 25ml @14:41:49.83 Vacuum too high -- waiting up to 40 seconds @14:41:49.84 SS.reconfigure :exhale @14:41:49.88 SS.hold @14:41:54.76 Intake @14.2psia, vacuum from 8.7 to 11.7psi @14:41:54.78 SS.reconfigure :inhaling 0.87ml/s @14:41:54.83 SS.seek 25ml @14:41:59.55 Stroke completed -- waiting for pressure to equalize ... @14:41:59.56 SS.reconfigure :exhale @14:41:59.61 SS.hold @14:42:06.04 Intake.select :close @14:42:06.18 SSV.dial :exhaust @14:42:06.95 Exhaust.select :open @14:42:07.06 SS.seek 0ml @14:42:16.05 Exhaust.select :close @14:42:16.16 SSV.dial CBV @14:42:16.18 CBV.dial SSV @14:42:16.96 Allowing 130s for 25.0ml of remaining 625.0ml @14:42:16.98 Intake.select :open @14:42:19.13 Intake @15.7psia, vacuum from 10.0 to 13.0psi @14:42:19.15 SS.reconfigure :inhaling 0.87ml/s @14:42:19.19 SS.seek 25ml @14:42:49.92 Vacuum too high -- waiting up to 40 seconds @14:42:49.93 SS.reconfigure :exhale @14:42:49.98 SS.hold @14:42:50.36 recoverPSRreagent08 @14:42:50.37 PTV.dial :puck @14:42:50.38 PBV.dial :waste1 @14:42:50.75 delay 30 seconds @14:42:54.16 Intake @14.2psia, vacuum from 8.7 to 11.7psi @14:42:54.17 SS.reconfigure :inhaling 0.78ml/s @14:42:54.22 SS.seek 25ml @14:42:54.72 Stroke completed -- waiting for pressure to equalize ... @14:42:54.74 SS.reconfigure :exhale @14:42:54.78 SS.hold @14:43:01.20 Intake.select :close @14:43:01.34 SSV.dial :exhaust @14:43:02.10 Exhaust.select :open @14:43:02.21 SS.seek 0ml @14:43:11.46 Exhaust.select :close @14:43:11.57 SSV.dial CBV @14:43:11.60 CBV.dial SSV @14:43:12.35 Allowing 130s for 25.0ml of remaining 600.0ml @14:43:12.37 Intake.select :open @14:43:14.52 Intake @15.6psia, vacuum from 10.0 to 13.0psi @14:43:14.54 SS.reconfigure :inhaling 0.78ml/s @14:43:14.59 SS.seek 25ml @14:43:20.77 PBV.dial between PSV and waste1 @14:43:21.14 PSR.select :air @14:43:21.16 PSV.dial PSR @14:43:21.91 PS.reconfigure :fast @14:43:21.96 PS.seek 2.5ml @14:43:45.81 PSV.dial PTV @14:43:46.58 delay 5 seconds @14:43:49.33 Stroke completed -- waiting for pressure to equalize ... @14:43:49.34 SS.reconfigure :exhale @14:43:49.39 SS.hold @14:43:51.59 PSV.dial PSR @14:43:52.36 delay 5 seconds @14:43:57.37 PSV.dial PTV @14:43:58.14 delay 5 seconds @14:43:59.06 Intake.select :close @14:43:59.20 SSV.dial :exhaust @14:43:59.96 Exhaust.select :open @14:44:00.07 SS.seek 0ml @14:44:03.15 PSV.dial :air @14:44:04.05 PS.seek 0ml @14:44:08.88 Exhaust.select :close @14:44:08.99 SSV.dial CBV @14:44:09.01 CBV.dial SSV @14:44:09.79 Allowing 130s for 25.0ml of remaining 575.0ml @14:44:09.80 Intake.select :open @14:44:11.96 Intake @15.6psia, vacuum from 10.0 to 13.0psi @14:44:11.98 SS.reconfigure :inhaling 0.78ml/s @14:44:12.02 SS.seek 25ml @14:44:27.47 getPMLReagent08 @14:44:27.48 PRV.dial :air @14:44:27.92 delay 5 seconds @14:44:32.94 PTV.dial :PRVmixing @14:44:34.26 delay 5 seconds @14:44:39.28 PRV.dial :ab2 @14:44:39.73 PTV.dial :PRVmixing @14:44:39.74 PSV.dial :mixloop @14:44:40.19 PS.reconfigure :default @14:44:40.23 PS.seek 0.3ml @14:44:44.18 delay 2 seconds @14:44:46.20 PRV.dial :air @14:44:46.71 PS.seek 0.55ml @14:44:46.86 Stroke completed -- waiting for pressure to equalize ... @14:44:46.87 SS.reconfigure :exhale @14:44:46.92 SS.hold @14:44:50.06 addPMLReagent08 @14:44:50.07 PSV.dial :air @14:44:50.53 PS.seek 2ml @14:44:57.95 Intake.select :close @14:44:58.09 SSV.dial :exhaust @14:44:58.85 Exhaust.select :open @14:44:58.96 SS.seek 0ml @14:45:06.12 PSV.dial :PML @14:45:06.57 PTV.dial :mixing @14:45:07.34 PBV.dial :waste1 @14:45:07.76 PS.seek 1.85ml @14:45:07.99 Exhaust.select :close @14:45:08.10 SSV.dial CBV @14:45:08.13 CBV.dial SSV @14:45:08.88 Allowing 130s for 25.0ml of remaining 550.0ml @14:45:08.89 Intake.select :open @14:45:10.06 PBV.dial between PSV and waste1 @14:45:10.94 PS.seek 0ml @14:45:11.05 Intake @15.7psia, vacuum from 10.0 to 13.0psi @14:45:11.07 SS.reconfigure :inhaling 0.78ml/s @14:45:11.11 SS.seek 25ml @14:45:30.65 delay 2 seconds @14:45:32.67 delay 8:00 @14:45:45.32 Stroke completed -- waiting for pressure to equalize ... @14:45:45.33 SS.reconfigure :exhale @14:45:45.38 SS.hold @14:45:57.29 Intake.select :close @14:45:57.43 SSV.dial :exhaust @14:45:58.19 Exhaust.select :open @14:45:58.30 SS.seek 0ml @14:46:07.13 Exhaust.select :close @14:46:07.24 SSV.dial CBV @14:46:07.27 CBV.dial SSV @14:46:08.02 Allowing 130s for 25.0ml of remaining 525.0ml @14:46:08.03 Intake.select :open @14:46:10.19 Intake @15.8psia, vacuum from 10.0 to 13.0psi @14:46:10.21 SS.reconfigure :inhaling 0.78ml/s @14:46:10.25 SS.seek 25ml @14:46:36.95 Vacuum too high -- waiting up to 40 seconds @14:46:36.96 SS.reconfigure :exhale @14:46:37.00 SS.hold @14:46:41.88 Intake @14.6psia, vacuum from 9.1 to 12.1psi @14:46:41.90 SS.reconfigure :inhaling 0.70ml/s @14:46:41.95 SS.seek 25ml @14:46:51.50 Stroke completed -- waiting for pressure to equalize ... @14:46:51.51 SS.reconfigure :exhale @14:46:51.56 SS.hold @14:47:00.02 Intake.select :close @14:47:00.16 SSV.dial :exhaust @14:47:00.92 Exhaust.select :open @14:47:01.03 SS.seek 0ml @14:47:10.06 Exhaust.select :close @14:47:10.17 SSV.dial CBV @14:47:10.19 CBV.dial SSV @14:47:10.96 Allowing 130s for 25.0ml of remaining 500.0ml @14:47:10.97 Intake.select :open @14:47:13.12 Intake @15.6psia, vacuum from 10.0 to 13.0psi @14:47:13.14 SS.reconfigure :inhaling 0.70ml/s @14:47:13.18 SS.seek 25ml @14:47:50.55 Stroke completed -- waiting for pressure to equalize ... @14:47:50.56 SS.reconfigure :exhale @14:47:50.60 SS.hold @14:48:03.67 Intake.select :close @14:48:03.83 SSV.dial :exhaust @14:48:04.59 Exhaust.select :open @14:48:04.71 SS.seek 0ml @14:48:13.50 Exhaust.select :close @14:48:13.61 SSV.dial CBV @14:48:13.63 CBV.dial SSV @14:48:14.41 Allowing 130s for 25.0ml of remaining 475.0ml @14:48:14.42 Intake.select :open @14:48:16.58 Intake @15.7psia, vacuum from 10.0 to 13.0psi @14:48:16.60 SS.reconfigure :inhaling 0.70ml/s @14:48:16.64 SS.seek 25ml @14:48:50.10 Vacuum too high -- waiting up to 40 seconds @14:48:50.11 SS.reconfigure :exhale @14:48:50.16 SS.hold @14:48:55.81 Intake @14.7psia, vacuum from 9.2 to 12.2psi @14:48:55.83 SS.reconfigure :inhaling 0.63ml/s @14:48:55.87 SS.seek 25ml @14:49:01.32 Stroke completed -- waiting for pressure to equalize ... @14:49:01.33 SS.reconfigure :exhale @14:49:01.37 SS.hold @14:49:09.83 Intake.select :close @14:49:09.98 SSV.dial :exhaust @14:49:10.73 Exhaust.select :open @14:49:10.85 SS.seek 0ml @14:49:19.65 Exhaust.select :close @14:49:19.76 SSV.dial CBV @14:49:19.78 CBV.dial SSV @14:49:20.56 Allowing 130s for 25.0ml of remaining 450.0ml @14:49:20.58 Intake.select :open @14:49:22.73 Intake @15.7psia, vacuum from 10.0 to 13.0psi @14:49:22.75 SS.reconfigure :inhaling 0.63ml/s @14:49:22.79 SS.seek 25ml @14:50:04.89 Stroke completed -- waiting for pressure to equalize ... @14:50:04.90 SS.reconfigure :exhale @14:50:04.94 SS.hold @14:50:17.60 Intake.select :close @14:50:17.74 SSV.dial :exhaust @14:50:18.50 Exhaust.select :open @14:50:18.61 SS.seek 0ml @14:50:27.64 Exhaust.select :close @14:50:27.75 SSV.dial CBV @14:50:27.78 CBV.dial SSV @14:50:28.53 Allowing 130s for 25.0ml of remaining 425.0ml @14:50:28.54 Intake.select :open @14:50:30.70 Intake @15.7psia, vacuum from 10.0 to 13.0psi @14:50:30.72 SS.reconfigure :inhaling 0.63ml/s @14:50:30.76 SS.seek 25ml @14:51:12.23 Stroke completed -- waiting for pressure to equalize ... @14:51:12.24 SS.reconfigure :exhale @14:51:12.28 SS.hold @14:51:26.72 Intake.select :close @14:51:26.86 SSV.dial :exhaust @14:51:27.62 Exhaust.select :open @14:51:27.73 SS.seek 0ml @14:51:36.73 Exhaust.select :close @14:51:36.84 SSV.dial CBV @14:51:36.86 CBV.dial SSV @14:51:37.64 Allowing 130s for 25.0ml of remaining 400.0ml @14:51:37.65 Intake.select :open @14:51:39.80 Intake @15.8psia, vacuum from 10.0 to 13.0psi @14:51:39.82 SS.reconfigure :inhaling 0.63ml/s @14:51:39.87 SS.seek 25ml @14:52:11.25 Vacuum too high -- waiting up to 40 seconds @14:52:11.26 SS.reconfigure :exhale @14:52:11.31 SS.hold @14:52:16.18 Intake @14.7psia, vacuum from 9.2 to 12.2psi @14:52:16.20 SS.reconfigure :inhaling 0.57ml/s @14:52:16.25 SS.seek 25ml @14:52:27.79 Stroke completed -- waiting for pressure to equalize ... @14:52:27.80 SS.reconfigure :exhale @14:52:27.84 SS.hold @14:52:39.00 Intake.select :close @14:52:39.14 SSV.dial :exhaust @14:52:39.90 Exhaust.select :open @14:52:40.01 SS.seek 0ml @14:52:48.80 Exhaust.select :close @14:52:48.91 SSV.dial CBV @14:52:48.93 CBV.dial SSV @14:52:49.71 Allowing 130s for 25.0ml of remaining 375.0ml @14:52:49.73 Intake.select :open @14:52:51.88 Intake @15.8psia, vacuum from 10.0 to 13.0psi @14:52:51.90 SS.reconfigure :inhaling 0.57ml/s @14:52:51.94 SS.seek 25ml @14:53:32.70 recoverPRVreagent08 @14:53:32.71 PTV.dial :mixing @14:53:32.72 PBV.dial :waste1 @14:53:33.10 delay 30 seconds @14:53:37.94 Stroke completed -- waiting for pressure to equalize ... @14:53:37.95 SS.reconfigure :exhale @14:53:37.99 SS.hold @14:53:52.25 Intake.select :close @14:53:52.39 SSV.dial :exhaust @14:53:53.15 Exhaust.select :open @14:53:53.26 SS.seek 0ml @14:54:02.02 Exhaust.select :close @14:54:02.13 SSV.dial CBV @14:54:02.15 CBV.dial SSV @14:54:03.11 PBV.dial between PSV and waste1 @14:54:03.47 Allowing 130s for 25.0ml of remaining 350.0ml @14:54:03.48 Intake.select :open @14:54:03.55 PSV.dial :air @14:54:04.16 PS.reconfigure :fast @14:54:04.21 PS.seek 2.5ml @14:54:05.65 Intake @15.7psia, vacuum from 10.0 to 13.0psi @14:54:05.67 SS.reconfigure :inhaling 0.57ml/s @14:54:05.71 SS.seek 25ml @14:54:28.14 PSV.dial :mixloop @14:54:28.59 delay 2 seconds @14:54:30.61 PSV.dial :air @14:54:31.08 delay 2 seconds @14:54:33.10 PSV.dial :mixloop @14:54:33.69 delay 2 seconds @14:54:35.70 PSV.dial :air @14:54:36.16 PS.seek 0ml @14:54:51.88 Stroke completed -- waiting for pressure to equalize ... @14:54:51.89 SS.reconfigure :exhale @14:54:51.94 SS.hold @14:54:59.48 washCycleDA #1 of 3 @14:54:59.49 PSR.select :air @14:54:59.51 PSV.dial PSR @14:55:00.89 Pulling 0.5ml of PSR.wash @14:55:00.90 PSR.select :wash @14:55:01.02 PS.reconfigure :default @14:55:01.06 PS.seek 0.5ml @14:55:07.08 delay 5 seconds @14:55:08.13 Intake.select :close @14:55:08.27 SSV.dial :exhaust @14:55:09.04 Exhaust.select :open @14:55:09.15 SS.seek 0ml @14:55:12.09 PSR.select :air @14:55:12.20 PS.seek 2ml @14:55:18.22 Exhaust.select :close @14:55:18.33 SSV.dial CBV @14:55:18.35 CBV.dial SSV @14:55:19.13 Allowing 130s for 25.0ml of remaining 325.0ml @14:55:19.15 Intake.select :open @14:55:21.30 Intake @15.7psia, vacuum from 10.0 to 13.0psi @14:55:21.32 SS.reconfigure :inhaling 0.57ml/s @14:55:21.36 SS.seek 25ml @14:55:28.42 PTV.dial :puck @14:55:29.17 PSV.dial PTV @14:55:29.92 PBV.dial :waste1 @14:55:30.35 PS.seek 1.5ml @14:55:36.26 delay 3 seconds @14:55:39.28 PBV.dial between PSV and waste1 @14:55:39.66 PS.seek 0ml @14:55:55.76 delay 2:00 @14:56:04.69 Vacuum too high -- waiting up to 40 seconds @14:56:04.70 SS.reconfigure :exhale @14:56:04.74 SS.hold @14:56:12.22 Intake @14.7psia, vacuum from 9.2 to 12.2psi @14:56:12.24 SS.reconfigure :inhaling 0.51ml/s @14:56:12.28 SS.seek 25ml @14:56:14.96 Stroke completed -- waiting for pressure to equalize ... @14:56:14.97 SS.reconfigure :exhale @14:56:15.01 SS.hold @14:56:24.77 Intake.select :close @14:56:24.91 SSV.dial :exhaust @14:56:25.68 Exhaust.select :open @14:56:25.79 SS.seek 0ml @14:56:34.86 Exhaust.select :close @14:56:34.97 SSV.dial CBV @14:56:34.99 CBV.dial SSV @14:56:35.77 Allowing 130s for 25.0ml of remaining 300.0ml @14:56:35.78 Intake.select :open @14:56:37.94 Intake @15.8psia, vacuum from 10.0 to 13.0psi @14:56:37.96 SS.reconfigure :inhaling 0.51ml/s @14:56:38.00 SS.seek 25ml @14:57:28.38 Stroke completed -- waiting for pressure to equalize ... @14:57:28.39 SS.reconfigure :exhale @14:57:28.43 SS.hold @14:57:44.62 Intake.select :close @14:57:44.76 SSV.dial :exhaust @14:57:45.52 Exhaust.select :open @14:57:45.63 SS.seek 0ml @14:57:54.42 Exhaust.select :close @14:57:54.53 SSV.dial CBV @14:57:54.55 CBV.dial SSV @14:57:55.32 Allowing 130s for 25.0ml of remaining 275.0ml @14:57:55.33 Intake.select :open @14:57:55.78 recoverPSRreagent08 @14:57:55.79 PTV.dial :puck @14:57:55.80 PBV.dial :waste1 @14:57:56.17 delay 30 seconds @14:57:57.48 Intake @15.7psia, vacuum from 10.0 to 13.0psi @14:57:57.50 SS.reconfigure :inhaling 0.51ml/s @14:57:57.54 SS.seek 25ml @14:58:26.19 PBV.dial between PSV and waste1 @14:58:26.56 PSR.select :air @14:58:26.58 PSV.dial PSR @14:58:27.34 PS.reconfigure :fast @14:58:27.39 PS.seek 2.5ml @14:58:48.74 Stroke completed -- waiting for pressure to equalize ... @14:58:48.75 SS.reconfigure :exhale @14:58:48.79 SS.hold @14:58:51.23 PSV.dial PTV @14:58:51.98 delay 5 seconds @14:58:56.99 PSV.dial PSR @14:58:57.74 delay 5 seconds @14:59:02.76 PSV.dial PTV @14:59:03.52 delay 5 seconds @14:59:07.08 Intake.select :close @14:59:07.24 SSV.dial :exhaust @14:59:08.00 Exhaust.select :open @14:59:08.11 SS.seek 0ml @14:59:08.54 PSV.dial :air @14:59:09.31 PS.seek 0ml @14:59:16.95 Exhaust.select :close @14:59:17.06 SSV.dial CBV @14:59:17.08 CBV.dial SSV @14:59:17.86 Allowing 130s for 25.0ml of remaining 250.0ml @14:59:17.88 Intake.select :open @14:59:20.03 Intake @15.7psia, vacuum from 10.0 to 13.0psi @14:59:20.05 SS.reconfigure :inhaling 0.51ml/s @14:59:20.09 SS.seek 25ml @14:59:32.76 washCycleDA #2 of 3 @14:59:33.20 PSR.select :air @14:59:33.30 PSV.dial PSR @14:59:34.67 Pulling 0.5ml of PSR.wash @14:59:34.68 PSR.select :wash @14:59:34.80 PS.reconfigure :default @14:59:34.84 PS.seek 0.5ml @14:59:40.73 delay 5 seconds @14:59:45.75 PSR.select :air @14:59:45.87 PS.seek 2ml @15:00:01.98 PTV.dial :puck @15:00:01.99 PSV.dial PTV @15:00:02.73 PBV.dial :waste1 @15:00:03.16 PS.seek 1.5ml @15:00:06.87 Vacuum too high -- waiting up to 40 seconds @15:00:06.88 SS.reconfigure :exhale @15:00:06.92 SS.hold @15:00:09.07 delay 3 seconds @15:00:12.09 PBV.dial between PSV and waste1 @15:00:12.51 PS.seek 0ml @15:00:15.50 Intake @15.1psia, vacuum from 9.6 to 12.6psi @15:00:15.52 SS.reconfigure :inhaling 0.47ml/s @15:00:15.56 SS.seek 25ml @15:00:20.76 Stroke completed -- waiting for pressure to equalize ... @15:00:20.78 SS.reconfigure :exhale @15:00:20.82 SS.hold @15:00:28.70 delay 2:00 @15:00:31.84 Intake.select :close @15:00:31.99 SSV.dial :exhaust @15:00:32.75 Exhaust.select :open @15:00:32.86 SS.seek 0ml @15:00:41.65 Exhaust.select :close @15:00:41.76 SSV.dial CBV @15:00:41.78 CBV.dial SSV @15:00:42.55 Allowing 130s for 25.0ml of remaining 225.0ml @15:00:42.56 Intake.select :open @15:00:44.73 Intake @15.8psia, vacuum from 10.0 to 13.0psi @15:00:44.74 SS.reconfigure :inhaling 0.47ml/s @15:00:44.79 SS.seek 25ml @15:01:40.95 Stroke completed -- waiting for pressure to equalize ... @15:01:40.96 SS.reconfigure :exhale @15:01:41.00 SS.hold @15:01:59.33 Intake.select :close @15:01:59.47 SSV.dial :exhaust @15:02:00.23 Exhaust.select :open @15:02:00.34 SS.seek 0ml @15:02:09.14 Exhaust.select :close @15:02:09.24 SSV.dial CBV @15:02:09.29 CBV.dial SSV @15:02:10.03 Allowing 130s for 25.0ml of remaining 200.0ml @15:02:10.04 Intake.select :open @15:02:12.20 Intake @15.9psia, vacuum from 10.0 to 13.0psi @15:02:12.22 SS.reconfigure :inhaling 0.47ml/s @15:02:12.26 SS.seek 25ml @15:02:28.72 recoverPSRreagent08 @15:02:28.73 PTV.dial :puck @15:02:28.74 PBV.dial :waste1 @15:02:29.10 delay 30 seconds @15:02:55.71 Vacuum too high -- waiting up to 40 seconds @15:02:55.72 SS.reconfigure :exhale @15:02:55.76 SS.hold @15:02:59.12 PBV.dial between PSV and waste1 @15:02:59.49 PSR.select :air @15:02:59.51 PSV.dial PSR @15:03:00.28 PS.reconfigure :fast @15:03:00.32 PS.seek 2.5ml @15:03:03.27 Intake @15.3psia, vacuum from 9.8 to 12.8psi @15:03:03.29 SS.reconfigure :inhaling 0.43ml/s @15:03:03.33 SS.seek 25ml @15:03:18.10 Stroke completed -- waiting for pressure to equalize ... @15:03:18.12 SS.reconfigure :exhale @15:03:18.16 SS.hold @15:03:24.03 PSV.dial PTV @15:03:24.79 delay 5 seconds @15:03:29.80 PSV.dial PSR @15:03:30.55 delay 5 seconds @15:03:30.73 Intake.select :close @15:03:30.87 SSV.dial :exhaust @15:03:31.62 Exhaust.select :open @15:03:31.73 SS.seek 0ml @15:03:35.57 PSV.dial PTV @15:03:36.33 delay 5 seconds @15:03:40.54 Exhaust.select :close @15:03:40.65 SSV.dial CBV @15:03:40.71 CBV.dial SSV @15:03:41.35 PSV.dial :air @15:03:41.44 Allowing 130s for 25.0ml of remaining 175.0ml @15:03:41.45 Intake.select :open @15:03:42.12 PS.seek 0ml @15:03:43.60 Intake @15.8psia, vacuum from 10.0 to 13.0psi @15:03:43.62 SS.reconfigure :inhaling 0.43ml/s @15:03:43.66 SS.seek 25ml @15:04:05.38 washCycleDA #3 of 3 @15:04:05.39 PSR.select :air @15:04:05.41 PSV.dial PSR @15:04:06.78 Pulling 0.5ml of PSR.wash @15:04:06.79 PSR.select :wash @15:04:06.90 PS.reconfigure :default @15:04:06.94 PS.seek 0.5ml @15:04:13.01 delay 5 seconds @15:04:18.02 PSR.select :air @15:04:18.14 PS.seek 2ml @15:04:34.31 PTV.dial :puck @15:04:34.33 PSV.dial PTV @15:04:35.07 PBV.dial :waste1 @15:04:35.50 PS.seek 1.5ml @15:04:41.47 delay 3 seconds @15:04:44.49 PBV.dial between PSV and waste1 @15:04:44.87 PS.seek 0ml @15:04:45.74 Stroke completed -- waiting for pressure to equalize ... @15:04:45.76 SS.reconfigure :exhale @15:04:45.80 SS.hold @15:05:01.07 delay 2:00 @15:05:04.03 Intake.select :close @15:05:04.17 SSV.dial :exhaust @15:05:04.93 Exhaust.select :open @15:05:05.04 SS.seek 0ml @15:05:14.09 Exhaust.select :close @15:05:14.20 SSV.dial CBV @15:05:14.23 CBV.dial SSV @15:05:14.98 Allowing 130s for 25.0ml of remaining 150.0ml @15:05:14.99 Intake.select :open @15:05:17.15 Intake @15.8psia, vacuum from 10.0 to 13.0psi @15:05:17.17 SS.reconfigure :inhaling 0.43ml/s @15:05:17.21 SS.seek 25ml @15:06:18.24 Stroke completed -- waiting for pressure to equalize ... @15:06:18.25 SS.reconfigure :exhale @15:06:18.30 SS.hold @15:06:40.62 Intake.select :close @15:06:40.76 SSV.dial :exhaust @15:06:41.52 Exhaust.select :open @15:06:41.64 SS.seek 0ml @15:06:50.48 Exhaust.select :close @15:06:50.59 SSV.dial CBV @15:06:50.62 CBV.dial SSV @15:06:51.38 Allowing 130s for 25.0ml of remaining 125.0ml @15:06:51.40 Intake.select :open @15:06:53.56 Intake @15.8psia, vacuum from 10.0 to 13.0psi @15:06:53.57 SS.reconfigure :inhaling 0.43ml/s @15:06:53.62 SS.seek 25ml @15:07:01.09 recoverPSRreagent08 @15:07:01.10 PTV.dial :puck @15:07:01.11 PBV.dial :waste1 @15:07:01.47 delay 30 seconds @15:07:31.50 PBV.dial between PSV and waste1 @15:07:31.86 PSR.select :air @15:07:31.88 PSV.dial PSR @15:07:32.65 PS.reconfigure :fast @15:07:32.69 PS.seek 2.5ml @15:07:47.98 Vacuum too high -- waiting up to 40 seconds @15:07:48.00 SS.reconfigure :exhale @15:07:48.04 SS.hold @15:07:56.37 PSV.dial PTV @15:07:57.13 delay 5 seconds @15:07:59.17 Intake @15.7psia, vacuum from 10.0 to 13.0psi @15:07:59.19 SS.reconfigure :inhaling 0.38ml/s @15:07:59.23 SS.seek 25ml @15:08:02.14 PSV.dial PSR @15:08:02.91 delay 5 seconds @15:08:07.93 PSV.dial PTV @15:08:08.13 Stroke completed -- waiting for pressure to equalize ... @15:08:08.14 SS.reconfigure :exhale @15:08:08.19 SS.hold @15:08:08.69 delay 5 seconds @15:08:13.78 PSV.dial :air @15:08:14.68 PS.seek 0ml @15:08:20.86 Intake.select :close @15:08:21.00 SSV.dial :exhaust @15:08:21.76 Exhaust.select :open @15:08:21.87 SS.seek 0ml @15:08:30.65 Exhaust.select :close @15:08:30.76 SSV.dial CBV @15:08:30.79 CBV.dial SSV @15:08:31.57 Allowing 130s for 25.0ml of remaining 100.0ml @15:08:31.59 Intake.select :open @15:08:33.74 Intake @15.9psia, vacuum from 10.0 to 13.0psi @15:08:33.76 SS.reconfigure :inhaling 0.38ml/s @15:08:33.80 SS.seek 25ml @15:08:38.00 Gate.power :camera,:ON @15:08:38.02 Mixing DA Substrates PSR.select :air @15:08:38.04 PSV.dial PSR @15:08:39.56 Pulling 0.25ml of PSR.sub1 @15:08:39.58 PSR.select :sub1 @15:08:39.78 PS.reconfigure :default @15:08:39.89 PS.seek 0.25ml @15:08:43.35 delay 5 seconds @15:08:48.43 Pulling 0.25ml of PSR.sub2 @15:08:48.44 PSR.select :sub2 @15:08:48.55 PS.seek 0.5ml @15:08:51.91 delay 5 seconds @15:08:56.93 PSR.select :air @15:08:57.03 PS.seek 2ml @15:09:13.25 PTV.dial :puck @15:09:13.26 PSV.dial PTV @15:09:14.03 PBV.dial :waste1 @15:09:14.47 PS.seek 1.5ml @15:09:20.40 delay 3 seconds @15:09:23.42 PBV.dial between PSV and waste1 @15:09:23.80 PS.seek 0ml @15:09:39.94 delay 10 seconds @15:09:44.61 Stroke completed -- waiting for pressure to equalize ... @15:09:44.63 SS.reconfigure :exhale @15:09:44.67 SS.hold @15:09:49.95 recoverPSRreagent08 @15:09:49.97 PTV.dial :puck @15:09:49.98 PBV.dial :waste1 @15:09:50.34 delay 30 seconds @15:10:04.98 Intake.select :close @15:10:05.13 SSV.dial :exhaust @15:10:05.90 Exhaust.select :open @15:10:06.01 SS.seek 0ml @15:10:14.80 Exhaust.select :close @15:10:14.91 SSV.dial CBV @15:10:14.93 CBV.dial SSV @15:10:15.71 Allowing 130s for 25.0ml of remaining 75.0ml @15:10:15.72 Intake.select :open @15:10:17.88 Intake @15.9psia, vacuum from 10.0 to 13.0psi @15:10:17.90 SS.reconfigure :inhaling 0.38ml/s @15:10:17.94 SS.seek 25ml @15:10:20.36 PBV.dial between PSV and waste1 @15:10:20.73 PSR.select :air @15:10:20.75 PSV.dial PSR @15:10:21.52 PS.reconfigure :fast @15:10:21.56 PS.seek 2.5ml @15:10:45.62 PSV.dial PTV @15:10:46.41 delay 5 seconds @15:10:51.43 PSV.dial PSR @15:10:52.20 delay 5 seconds @15:10:57.22 PSV.dial PTV @15:10:58.00 delay 5 seconds @15:11:03.02 PSV.dial :air @15:11:03.78 PS.seek 0ml @15:11:27.34 Additional air clear @15:11:27.35 PSR.select :air @15:11:27.37 PSV.dial PSR @15:11:28.42 Stroke completed -- waiting for pressure to equalize ... @15:11:28.43 SS.reconfigure :exhale @15:11:28.48 SS.hold @15:11:28.79 Pulling 2ml of PSR.air @15:11:28.80 PSR.select :air @15:11:28.83 PS.reconfigure :default @15:11:28.87 PS.seek 2ml @15:11:50.12 delay 5 seconds @15:11:51.35 Intake.select :close @15:11:51.50 SSV.dial :exhaust @15:11:52.25 Exhaust.select :open @15:11:52.36 SS.seek 0ml @15:11:55.14 PTV.dial :puck @15:11:55.15 PSV.dial PTV @15:11:55.89 PBV.dial :waste1 @15:11:56.30 PS.seek 1.5ml @15:12:01.41 Exhaust.select :close @15:12:01.52 SSV.dial CBV @15:12:01.55 CBV.dial SSV @15:12:02.28 delay 3 seconds @15:12:02.32 Allowing 130s for 25.0ml of remaining 50.0ml @15:12:02.33 Intake.select :open @15:12:04.48 Intake @15.9psia, vacuum from 10.0 to 13.0psi @15:12:04.50 SS.reconfigure :inhaling 0.38ml/s @15:12:04.54 SS.seek 25ml @15:12:05.30 PBV.dial between PSV and waste1 @15:12:05.67 delay 1 second @15:12:06.69 PSV.dial :waste1 @15:12:07.75 PS.seek 0ml @15:12:23.92 Processing Puck Heater at 28C before imaging @15:12:23.93 PH.coast @15:12:24.05 Imaging da puck in Processing Clamp while storing it to tube 2 @15:12:24.22 SC.reconfigure :tight @15:12:24.26 SC.seek 2 @15:12:27.81 SC.configure :limp @15:12:28.01 Elbow.reconfigure :tight @15:12:28.05 Elbow.seek 2 @15:12:29.80 Forearm.reconfigure :tight @15:12:29.85 Forearm.seek 2 + 450 counts @15:12:30.88 SE.reconfigure :pick @15:12:30.93 SE.seek :beamBreak @15:12:33.97 SE.configure :default @15:12:34.13 Elbow.reconfigure :limp @15:12:34.17 Forearm.reconfigure :loose @15:12:34.21 Forearm.seek 2 @15:12:34.42 Elbow.hold @15:12:34.49 Elbow.reconfigure :tight @15:12:34.74 SE.seek 0.16 Pucks below Top Plate @15:12:35.15 Forearm.force -20 @15:12:35.19 Forearm.seek 2 + 300 counts @15:12:35.48 Forearm.reconfigure :tight @15:12:35.52 Forearm.seek 2 + 450 counts @15:12:35.71 SE.seek 1.16 Pucks below Top Plate @15:12:36.20 Forearm.reconfigure :loose @15:12:36.24 Forearm.seek :retract @15:12:37.16 Elbow.seek PC @15:12:39.71 Elbow.reconfigure :loose @15:12:39.81 Forearm.seek PC @15:12:40.84 Hand.close @15:12:41.51 PC.seek :open @15:12:46.28 Forearm.seek :retract @15:12:47.21 Elbow.reconfigure :tight @15:12:47.25 Elbow.seek 2 @15:12:49.91 Forearm.reconfigure :tight @15:12:49.96 Forearm.seek 2 + 450 counts @15:12:50.92 Hand.open @15:12:51.56 SE.seek 0.81 Pucks below Top Plate @15:12:51.82 SE.reconfigure :seal @15:12:52.38 SE.seek -0.15 Pucks below Top Plate @15:12:52.94 Lights, camera, ACTION! @15:12:52.97 PuckCamera: snap -bin=2,2 -auto /var/log/esp/da23jan0315h275ml.tif @15:13:09.78 Vacuum too high -- waiting up to 40 seconds @15:13:09.79 SS.reconfigure :exhale @15:13:09.84 SS.hold @15:13:12.44 Waiting >20 seconds for da to relinquish FlushPuck @15:13:14.99 SX HX9/16 (USB:09): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 18.695 seconds /var/log/esp/da23jan0315h275ml.tif: TIFF Upload Complete @15:13:15.01 PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da23jan0315h275ml15s.tif @15:13:20.89 Intake @15.6psia, vacuum from 10.0 to 13.0psi @15:13:20.91 SS.reconfigure :inhaling 0.34ml/s @15:13:20.96 SS.seek 25ml @15:13:27.11 Stroke completed -- waiting for pressure to equalize ... @15:13:27.12 SS.reconfigure :exhale @15:13:27.17 SS.hold @15:13:30.67 SX HX9/16 (USB:09): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15 seconds /var/log/esp/midres/da23jan0315h275ml15s.tif: TIFF Upload Complete @15:13:30.73 PuckCamera: snap -bin=2,2 20.0 /var/log/esp/midres/da23jan0315h275ml20s.tif @15:13:41.56 Intake.select :close @15:13:41.70 SSV.dial :exhaust @15:13:42.47 Exhaust.select :open @15:13:42.58 SS.seek 0ml @15:13:51.38 SX HX9/16 (USB:09): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 20 seconds /var/log/esp/midres/da23jan0315h275ml20s.tif: TIFF Upload Complete @15:13:51.40 Gate.power :camera,:OFF @15:13:51.44 DAfans.email "18.695 sec auto-exposure",Subject: "da23jan0315h275ml images!" @15:13:51.47 Exhaust.select :close @15:13:51.76 SSV.dial CBV @15:13:51.78 CBV.dial SSV @15:13:51.86 Elbow.seek 2 @15:13:52.05 Forearm.seek 2 + 450 counts @15:13:52.12 SE.reconfigure :gently @15:13:52.16 SE.seek 0.57 Pucks below Top Plate @15:13:52.57 Allowing 130s for 25.0ml of remaining 25.0ml @15:13:52.58 Intake.select :open @15:13:52.79 Elbow.reconfigure :limp @15:13:52.84 Forearm.reconfigure :loose @15:13:52.89 Forearm.seek 2 @15:13:53.11 Elbow.hold @15:13:53.19 Elbow.reconfigure :tight @15:13:53.24 SE.reconfigure :default @15:13:53.30 SE.seek 0.75 Pucks below Top Plate @15:13:53.70 Forearm.force -20 @15:13:53.73 Forearm.seek 2 + 312 counts @15:13:54.03 Forearm.reconfigure :tight @15:13:54.09 Forearm.seek 2 + 450 counts @15:13:54.37 SE.seek :down @15:13:54.83 Intake @16.1psia, vacuum from 10.0 to 13.0psi @15:13:54.85 SS.reconfigure :inhaling 0.34ml/s @15:13:54.89 SS.seek 24.98ml @15:13:55.65 Forearm.reconfigure :loose @15:13:55.70 Forearm.seek :retract @15:13:57.03 SE.stop @15:13:57.17 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @15:13:57.58 Elbow.seek FlushPuck::Garage @15:13:59.62 Elbow.reconfigure :loose @15:13:59.81 Forearm.seek :garage @15:14:00.94 Hand.close @15:14:01.96 Forearm.seek :retract @15:14:02.98 Elbow.reconfigure :tight @15:14:03.08 Elbow.seek PC @15:14:04.66 Elbow.reconfigure :loose @15:14:04.85 Forearm.reconfigure :tight @15:14:04.93 Forearm.seek PC @15:14:06.45 PC.seek :closed @15:14:11.20 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.56 @15:14:11.35 Hand.open @15:14:12.15 Forearm.reconfigure :loose @15:14:12.24 Forearm.seek :retract @15:14:13.27 PSR.select :air @15:14:13.31 PSV.dial PSR @15:14:14.00 Pulling 0.5ml of PSR.flush @15:14:14.03 PSR.select :flush @15:14:14.22 PS.seek 0.5ml @15:14:20.19 delay 5 seconds @15:14:25.23 PSR.select :air @15:14:25.42 PS.seek 2.5ml @15:14:26.39 upsync @15:14:46.65 delay 1 second @15:14:47.67 PRV.dial :waste2,avoiding: :ab2 @15:14:49.55 PTV.dial :PRVmixing @15:14:50.88 PSV.dial :mixloop @15:14:51.92 PS.seek :empty @15:15:17.51 Stroke completed -- waiting for pressure to equalize ... @15:15:17.53 SS.reconfigure :exhale @15:15:17.57 SS.hold @15:15:18.27 PSR.select :air @15:15:18.29 PSV.dial PSR @15:15:19.39 Pulling 0.5ml of PSR.flush @15:15:19.40 PSR.select :flush @15:15:19.57 PS.seek 0.5ml @15:15:25.49 delay 5 seconds @15:15:30.51 PSR.select :air @15:15:30.62 PS.seek 2.5ml @15:15:37.92 Intake.select :close @15:15:38.06 SSV.dial :exhaust @15:15:38.83 Exhaust.select :open @15:15:38.94 SS.seek 0ml @15:15:47.87 Exhaust.select :close @15:15:47.98 Sampled 1000.0ml @15:15:48.00 Drying puck before fixing for 20:00 @15:15:48.01 CBV.dial :waste2b @15:15:48.76 delay 1:00 @15:15:51.83 delay 1 second @15:15:52.85 PBV.dial :waste1 @15:15:53.23 PTV.dial :mixing @15:15:53.98 PSV.dial :mixloop @15:15:55.03 PS.seek :empty @15:16:21.33 PSV.dial PSR @15:16:22.90 PSR.select :flush @15:16:23.01 PS.seek 1ml @15:16:34.02 PSR.select :air @15:16:34.13 PS.seek 2.5ml @15:16:48.78 CSV.dial :air @15:16:49.23 CS.reconfigure :fast @15:16:49.28 CS.seek 10ml @15:16:50.30 PSV.dial PTV @15:16:51.07 PTV.dial :puck @15:16:51.82 PBV.dial :waste1 @15:16:51.84 PS.seek 0ml @15:17:13.53 Pumping wcr puck from tube 3 up to 35psi... @15:17:13.54 CBV.dial SSV @15:17:14.86 CBV.dial between CSV and waste2b @15:17:15.80 CSV.dial between air and CTV @15:17:16.13 CS.seek 7.062ml @15:17:18.21 PSV.dial PSR @15:17:18.98 PSR.select :flush @15:17:19.10 PS.seek 0.5ml @15:17:23.69 CTV.dial CSV,avoiding: :air @15:17:23.71 CSV.dial CTV @15:17:24.52 CS.reconfigure :pressurize @15:17:24.56 CS.seek 0ml @15:17:24.99 PSR.select :air @15:17:25.10 PS.seek 2.5ml @15:17:42.51 CBV.dial :waste2b @15:17:42.84 CS.reconfigure :default @15:17:42.89 CS.seek 0ml @15:17:44.21 delay 1:00 @15:17:46.43 PSV.dial PTV @15:17:47.19 PBV.dial PSV @15:17:47.78 PS.seek 0ml @15:18:14.33 PSV.dial PBV @15:18:14.81 PS.seek 2.5ml @15:18:41.30 PSV.dial :waste1 @15:18:42.04 PS.seek 0ml @15:18:44.23 CTV.dial :air,avoiding: :intake @15:18:45.56 Fix #1 of 2 (with RNAlater) for 10:00 @15:18:45.57 CSV.dial CSR @15:18:46.06 Pulling 2ml of CSR.RNAlater @15:18:46.07 CSR.select :RNAlater @15:18:46.18 CS.seek 2ml @15:18:52.12 delay 5 seconds @15:18:57.14 CSR.select :air @15:18:57.25 CS.reconfigure :fast @15:18:57.29 CS.seek 10ml @15:19:08.39 PSV.dial PSR @15:19:08.86 PSR.select :air @15:19:08.89 PS.seek 2.5ml @15:19:16.77 Pumping wcr puck from tube 3 up to 35psi... @15:19:16.79 CBV.dial SSV @15:19:18.09 CBV.dial between CSV and waste2b @15:19:19.03 CSV.dial between air and CTV @15:19:19.65 CS.seek 7.128ml @15:19:26.97 CTV.dial CSV,avoiding: :air @15:19:26.99 CSV.dial CTV @15:19:28.39 CS.reconfigure :pressurize @15:19:28.43 CS.seek 0ml @15:19:35.20 PSV.dial PTV @15:19:35.94 PBV.dial PSV @15:19:35.96 PS.seek 0ml @15:19:45.42 delay 10:00 @15:20:02.49 PSV.dial PBV @15:20:02.96 PS.seek 2.5ml @15:20:29.30 PSV.dial :waste1 @15:20:30.04 PS.seek 0ml @15:20:56.36 PSV.dial PSR @15:20:56.83 PSR.select :flush @15:20:56.94 PS.seek 1ml @15:21:07.95 PSR.select :air @15:21:08.06 PS.seek 2.5ml @15:21:24.23 PSV.dial PTV @15:21:25.42 PTV.dial :puck @15:21:25.44 PBV.dial :waste1 @15:21:26.01 PS.seek 0ml @15:21:52.39 PSV.dial PSR @15:21:53.14 PSR.select :flush @15:21:53.26 PS.seek 0.5ml @15:21:59.14 PSR.select :air @15:21:59.25 PS.seek 2.5ml @15:22:20.55 PSV.dial PTV @15:22:21.31 PBV.dial PSV @15:22:21.88 PS.seek 0ml @15:22:48.23 PSV.dial PBV @15:22:48.71 PS.seek 2.5ml @15:23:15.11 PSV.dial :waste1 @15:23:15.85 PS.seek 0ml @15:23:42.23 PSV.dial PSR @15:23:42.70 PSR.select :air @15:23:42.73 PS.seek 2.5ml @15:24:09.17 PSV.dial PTV @15:24:09.90 PBV.dial PSV @15:24:09.92 PS.seek 0ml @15:24:36.29 PSV.dial PBV @15:24:36.77 PS.seek 2.5ml @15:25:03.07 PSV.dial :waste1 @15:25:03.82 PS.seek 0ml @15:25:30.17 Garaging FlushPuck in Processing Clamp @15:25:30.24 Elbow.reconfigure :tight @15:25:30.29 Elbow.seek PC @15:25:30.39 Elbow.reconfigure :loose @15:25:30.48 Forearm.seek PC @15:25:31.44 Hand.close @15:25:32.00 PC.seek :open @15:25:36.71 Forearm.seek :retract @15:25:37.71 Elbow.reconfigure :tight @15:25:37.76 Elbow.seek :garage @15:25:39.09 Elbow.reconfigure :loose @15:25:39.18 Forearm.reconfigure :tight @15:25:39.23 Forearm.seek :garage @15:25:40.27 Hand.open @15:25:40.93 Forearm.reconfigure :loose @15:25:40.97 Forearm.seek :retract @15:29:45.43 CBV.dial :waste2b @15:29:45.77 CS.reconfigure :default @15:29:45.81 CS.seek 0ml @15:29:48.23 delay 1:00 @15:30:48.26 CTV.dial :air,avoiding: :intake @15:30:49.58 Fix #2 of 2 (with RNAlater) for 10:00 @15:30:49.59 CSV.dial CSR @15:30:50.08 Pulling 2ml of CSR.RNAlater @15:30:50.09 CSR.select :RNAlater @15:30:50.20 CS.seek 2ml @15:30:56.11 delay 5 seconds @15:31:01.13 CSR.select :air @15:31:01.24 CS.reconfigure :fast @15:31:01.28 CS.seek 10ml @15:31:20.86 Pumping wcr puck from tube 3 up to 35psi... @15:31:20.87 CBV.dial SSV @15:31:22.19 CBV.dial between CSV and waste2b @15:31:23.15 CSV.dial between air and CTV @15:31:23.75 CS.seek 7.149ml @15:31:31.08 CTV.dial CSV,avoiding: :air @15:31:31.10 CSV.dial CTV @15:31:32.49 CS.reconfigure :pressurize @15:31:32.53 CS.seek 0ml @15:31:46.69 delay 10:00 @15:41:46.71 CBV.dial :waste2b @15:41:47.04 CS.reconfigure :default @15:41:47.08 CS.seek 0ml @15:41:52.69 delay 1:00 @15:42:52.72 CTV.dial :air,avoiding: :intake @15:42:54.11 Storing wcr puck in Collection Clamp to tube 2 @15:42:54.27 SC.reconfigure :tight @15:42:54.31 SC.seek 2 @15:42:54.96 SC.configure :limp @15:42:55.17 Elbow.reconfigure :tight @15:42:55.21 Elbow.seek 2 @15:42:57.10 Forearm.reconfigure :tight @15:42:57.14 Forearm.seek 2 + 450 counts @15:42:58.16 SE.reconfigure :pick @15:42:58.21 SE.seek :beamBreak @15:43:01.25 SE.configure :default @15:43:01.40 Elbow.reconfigure :limp @15:43:01.45 Forearm.reconfigure :loose @15:43:01.49 Forearm.seek 2 @15:43:01.71 Elbow.hold @15:43:01.78 Elbow.reconfigure :tight @15:43:01.87 SE.seek 1.17 Pucks below Top Plate @15:43:02.31 Forearm.force -20 @15:43:02.34 Forearm.seek 2 + 311 counts @15:43:02.63 Forearm.reconfigure :tight @15:43:02.67 Forearm.seek 2 + 450 counts @15:43:02.86 SE.seek 2.17 Pucks below Top Plate @15:43:03.34 Forearm.reconfigure :loose @15:43:03.38 Forearm.seek :retract @15:43:04.61 Elbow.seek CC @15:43:06.21 Elbow.reconfigure :loose @15:43:06.30 Forearm.seek CC @15:43:07.28 Hand.close @15:43:07.86 CC.seek :open @15:43:12.62 Forearm.seek :retract @15:43:13.67 Elbow.reconfigure :tight @15:43:13.71 Elbow.seek 2 @15:43:15.51 Elbow.reconfigure :limp @15:43:15.56 Forearm.seek 2 @15:43:16.49 Elbow.hold @15:43:16.56 Elbow.reconfigure :tight @15:43:16.61 Hand.open @15:43:17.34 Forearm.force -20 @15:43:17.38 Forearm.seek 2 + 296 counts @15:43:17.67 Forearm.reconfigure :tight @15:43:17.72 Forearm.seek 2 + 450 counts @15:43:17.97 SE.seek :down @15:43:19.10 Forearm.reconfigure :loose @15:43:19.14 Forearm.seek :retract @15:43:20.52 SE.stop @15:43:20.56
Starting finalPurge @15:43:20.57 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @15:43:20.75 Elbow.seek FlushPuck::Garage @15:43:22.60 Elbow.reconfigure :loose @15:43:22.69 Forearm.seek :garage @15:43:23.62 Hand.close @15:43:24.34 Forearm.seek :retract @15:43:25.31 Elbow.reconfigure :tight @15:43:25.35 Elbow.seek CC @15:43:28.27 Elbow.reconfigure :loose @15:43:28.36 Forearm.reconfigure :tight @15:43:28.40 Forearm.seek CC @15:43:29.65 CC.seek :closed @15:43:34.18 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.26 @15:43:34.25 Hand.open @15:43:34.91 Forearm.reconfigure :loose @15:43:34.96 Forearm.seek :retract @15:43:35.93 Flushing the Collection Valve::Manifold @15:43:36.05 CSV.dial CSR @15:43:36.54 Pulling 5ml of CSR.flush @15:43:36.55 CSR.select :flush @15:43:36.66 CS.seek 5ml @15:43:50.25 delay 5 seconds @15:43:55.27 CSR.select :air @15:43:55.41 CS.reconfigure :fast @15:43:55.46 CS.seek 7.501ml @15:44:02.25 CSV.dial :waste @15:44:03.52 CS.reconfigure :default @15:44:03.56 CS.seek 0ml @15:44:23.66 Flushing lower manifold's waste1 port @15:44:23.77 CSV.dial :flush @15:44:24.22 CS.reconfigure :fast @15:44:24.27 CS.seek 5ml @15:44:37.06 delay 5 seconds @15:44:42.08 CSV.dial :air @15:44:42.57 CS.seek 7ml @15:44:48.16 CSV.dial CTV @15:44:48.19
CTV.dial CSV @15:44:49.56 CBV.dial :waste1 @15:44:50.32 CS.seek 0ml @15:45:06.84 Flushing lower manifold's waste2 port @15:45:06.96 CSV.dial :flush @15:45:07.69 CS.seek 5ml @15:45:20.43 delay 5 seconds @15:45:25.45 CSV.dial :air @15:45:25.96 CS.seek 7ml @15:45:31.47 CSV.dial CTV @15:45:31.49
CTV.dial CSV @15:45:31.94 CBV.dial :waste2 @15:45:32.99 CS.seek 0ml @15:45:49.53 Flushing lower manifold's waste2b port @15:45:49.64 CSV.dial :flush @15:45:50.38 CS.seek 5ml @15:46:03.00 delay 5 seconds @15:46:08.02 CSV.dial :air @15:46:08.53 CS.seek 7.001ml @15:46:14.09 CSV.dial CTV @15:46:14.11
CTV.dial CSV @15:46:14.56 CBV.dial :waste2b @15:46:15.61 CS.seek 0ml @15:46:32.15 Disinfecting external Intake with kill @15:46:32.27 CSV.dial CSR @15:46:32.88 Pulling 8ml of CSR.kill @15:46:32.89 CSR.select :kill @15:46:33.00 CS.seek 8ml @15:46:52.78 delay 5 seconds @15:46:57.80 CSR.select :air @15:46:57.91 CS.seek 10ml @15:47:03.40 CSV.dial CBV @15:47:03.45
CBV.dial CSV @15:47:04.48 CTV.dial :intake @15:47:05.24 Intake.select :open @15:47:05.35 CS.seek 2ml @15:47:24.27 Intake.select :close @15:47:24.37 CTV.dial :air @15:47:25.14 CS.reconfigure :default @15:47:25.18 CS.seek 0ml @15:47:31.15 CSV.dial CSR @15:47:32.20 Pulling 5ml of CSR.flush @15:47:32.21 CSR.select :flush @15:47:32.33 CS.seek 5ml @15:47:45.94 delay 5 seconds @15:47:50.95 CSR.select :air @15:47:51.07 CS.seek 10ml @15:48:04.62 CSV.dial CBV @15:48:05.61 CS.seek 0ml @15:48:32.00 Garaging FlushPuck in Collection Clamp @15:48:32.06 Elbow.reconfigure :tight @15:48:32.10 Elbow.seek CC @15:48:32.43 Elbow.reconfigure :loose @15:48:32.52 Forearm.seek CC @15:48:33.47 Hand.close @15:48:34.10 CC.seek :open @15:48:38.70 Forearm.seek :retract @15:48:39.67 Elbow.reconfigure :tight @15:48:39.71 Elbow.seek :garage @15:48:42.69 Elbow.reconfigure :loose @15:48:42.78 Forearm.reconfigure :tight @15:48:42.82 Forearm.seek :garage @15:48:43.72 Hand.open @15:48:44.86 Forearm.reconfigure :loose @15:48:44.92 Forearm.seek :retract @15:48:45.88 Completed [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] begun 13:22:19 @15:48:45.93 Asleep after completing DA_WCR_1323 assays @15:48:45.95 GoodNews.email "{1 => 33,\n 2 => 3,\n 3 => 30,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@15:41:29, 18% flow, 15.6C, 30% humidity, 14.6psia, 13.754V, 0.387A, 0.375A avg, 5.32W\nSampled 1000.0ml",Subject: "Asleep after completing DA_WCR_1323 assays" @15:48:51.97 upsync @13:40:05.21UTC09-Jan-23
ESP2 v3.41 I2C Gateway v3.20 for core bus @13:40:07.79 Configuration for short puck jake 1/11/21 by brent@mbari.org /home/esp/esp2/type/shallow/jake/configure.rb @13:40:11.75 Polling Can every 10:00 @13:40:11.85 Cleanly exited at 13:39:33UTC09-Jan-23 @13:40:11.89 Loading /home/esp/esp2/mission/DA_WCR_1923.rb mission @13:40:11.94 Preparing to sleep until + 00:00:05 @13:40:11.96 GoodNews.email "Starting DA_WCR_1923 w/pucks in tube 3\n{1 => 33,\n 2 => 3,\n 3 => 30,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@13:40:11, 17% flow, 16.1C, 31% humidity, 14.6psia, 13.778V, 0.278A, 0.266A avg, 3.83W",Subject: "DA_WCR_1923 begun" @13:40:12.00
Asleep til + 00:00:05 @13:40:12.04 Continuing at 13:40 @13:40:12.28 Gate.power :core,:OFF @13:40:12.30 Gate.power :raw,:OFF Shutdown @13:40:16.42 GoodNews.email "Then, in tube 3: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2/mission/DA_WCR_1923.rb:14\n{1 => 33,\n 2 => 3,\n 3 => 30,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@13:40:12, 17% flow, 16.2C, 31% humidity, 14.6psia, 13.773V, 0.278A, 0.266A avg, 3.83W",Subject: "Asleep until 13:40" @13:40:16.92
Powering core bus @13:40:18.23 upsync @13:40:21.70 upsync @13:40:22.15
Gate.power :raw,:ON @13:40:22.84 CS.configure :default @13:40:22.93 PS.configure :default @13:40:23.02 SS.configure :default @13:40:23.11 CC.configure :default @13:40:23.20 PC.configure :default @13:40:23.30 Forearm.configure :loose @13:40:23.39 Elbow.configure :loose @13:40:23.49 SC.configure :loose @13:40:23.58 SE.configure :default @13:40:23.62 CH.coast @13:40:23.67 CH.configure :default @13:40:23.70 PH.coast @13:40:23.75 PH.configure :default @13:40:23.78 All dwarf controllers running firmware version 3.85 @13:40:23.89 Valve.reset @13:40:24.15 Intake.select :close @13:40:24.17 Exhaust.select :close @13:40:24.18 CTV.dial :air @13:40:24.27 CSV.dial :waste1 @13:40:24.31 PSV.dial :air @13:40:24.41
Forearm.reconfigure :loose @13:40:24.45 Forearm.home.seek :retract @13:40:24.78 SSV.dial :spit,avoiding: :exhaust @13:40:25.15 Hand.close @13:40:25.18
Elbow.jog 1500 @13:40:25.39 PS.reconfigure :default @13:40:25.44 PS.home.seek 0ml @13:40:25.60 CS.reconfigure :fast @13:40:25.62 SS.reconfigure :default @13:40:25.72 CS.home.seek 0ml @13:40:25.75 SS.home.seek 0ml @13:40:25.87
Elbow.reconfigure :loose @13:40:25.91 Elbow.home.seek :clear @13:40:30.46 SE.reconfigure :default @13:40:30.50 SE.home.seek :down @13:40:31.10 SC.reconfigure :tight @13:40:31.15 SC.home.seek :initial @13:40:33.69 PS.stop @13:40:59.44
SC.configure :limp @13:40:59.49 Forearm.seek :retract @13:40:59.55 Elbow.seek :hammer @13:41:00.59 Forearm.reconfigure :tight @13:41:00.63 Forearm.seek retracted @13:41:01.03 PC.reconfigure :default @13:41:01.05
CC.reconfigure :default @13:41:01.13 PC.home.seek :open @13:41:01.15
CC.home.seek :open @13:41:04.66 Phase [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] @13:41:04.67 Notice.email "At 13:41:04 began in tube 3: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2/mission/DA_WCR_1923.rb:14\n{1 => 33,\n 2 => 3,\n 3 => 30,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@13:41:04, 17% flow, 16.1C, 31% humidity, 14.6psia, 13.769V, 0.296A, 0.284A avg, 4.08W",Subject: "Started DA_WCR_1923 phase initialPurge; da [1000, 1000] #da and wsr are run simultanelously" @13:41:04.69
Starting initialPurge @13:41:04.71 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @13:41:04.78 Hand.open @13:41:05.49 Elbow.reconfigure :tight @13:41:05.53 Elbow.seek FlushPuck::Garage @13:41:07.41 Elbow.reconfigure :loose @13:41:07.50 Forearm.reconfigure :loose @13:41:07.54 Forearm.seek :garage @13:41:08.47 Hand.close @13:41:09.27 Forearm.seek :retract @13:41:09.90 upsync @13:41:10.23
Elbow.reconfigure :tight @13:41:10.28 Elbow.seek CC @13:41:13.36 Elbow.reconfigure :loose @13:41:13.45 Forearm.reconfigure :tight @13:41:13.49 Forearm.seek CC @13:41:14.69 CC.seek :closed @13:41:19.21 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.38 @13:41:19.28 Hand.open @13:41:19.91 Forearm.reconfigure :loose @13:41:19.96 Forearm.seek :retract @13:41:20.89 Flushing the Collection Valve::Manifold @13:41:21.00 CSV.dial CSR @13:41:22.33 Pulling 5ml of CSR.flush CSR.select :flush @13:41:22.44 CS.reconfigure :default @13:41:22.48 CS.seek 5ml @13:41:36.13 delay 5 seconds @13:41:41.14 CSR.select :air @13:41:41.30 CS.reconfigure :fast @13:41:41.34 CS.seek 7.5ml @13:41:48.11 CSV.dial :waste @13:41:49.38 CS.reconfigure :default @13:41:49.43 CS.seek 0ml @13:42:09.40 Purging external Intake @13:42:09.51 CSV.dial :flush @13:42:09.95 CS.reconfigure :fast @13:42:09.99 CS.seek 10ml @13:42:34.39 delay 5 seconds @13:42:39.42 CSV.dial CBV @13:42:39.44
CBV.dial CSV @13:42:40.19 CTV.dial :intake @13:42:41.00 Intake.select :open @13:42:41.11 CS.seek 0ml @13:43:04.70 Intake.select :close @13:43:04.80 CTV.dial :air @13:43:05.60 Flushing upper manifold @13:43:05.71 CSV.dial :flush @13:43:06.45 CS.seek 5ml @13:43:19.27 delay 5 seconds @13:43:24.29 CSV.dial :air @13:43:24.78 CS.seek 7ml @13:43:30.30 CSV.dial CBV @13:43:30.32
CBV.dial CSV @13:43:31.35 CTV.dial :air @13:43:31.36 CS.seek 0ml @13:43:47.99 delay 7 seconds @13:43:55.01 CS.seek 3ml @13:44:02.98 CSV.dial :waste @13:44:03.43 CS.seek 0ml @13:44:11.15 CSV.dial :flush @13:44:11.59 CS.seek 3ml @13:44:19.52 delay 5 seconds @13:44:24.53 CSV.dial :air @13:44:25.15 CS.seek 5ml @13:44:30.83 CSV.dial :air @13:44:30.84 CS.seek 0ml @13:44:43.08 delay 7 seconds @13:44:50.11 CS.seek 3ml @13:44:58.01 CSV.dial :waste @13:44:58.72 CS.seek 0ml @13:45:06.39 Garaging FlushPuck in Collection Clamp @13:45:06.46 Elbow.reconfigure :tight @13:45:06.51 Elbow.seek CC @13:45:06.62 Elbow.reconfigure :loose @13:45:06.71 Forearm.seek CC @13:45:07.66 Hand.close @13:45:08.23 CC.seek :open @13:45:12.87 Forearm.seek :retract @13:45:13.82 Elbow.reconfigure :tight @13:45:13.86 Elbow.seek :garage @13:45:16.85 Elbow.reconfigure :loose @13:45:16.94 Forearm.reconfigure :tight @13:45:16.99 Forearm.seek :garage @13:45:17.93 Hand.open @13:45:19.09 Forearm.reconfigure :loose @13:45:19.13 Forearm.seek :retract @13:45:20.10 da sampling at most 1000ml, wcr at most 1000ml @13:45:20.13 Loading sh1 puck from tube 3 into Collection Clamp @13:45:20.15 DAfans.email "Started da in tube 3 at 13:45:20UTC09-Jan-23\n{1 => 33,\n 2 => 3,\n 3 => 30,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@13:41:04, 17% flow, 16.1C, 31% humidity, 14.6psia, 13.769V, 0.296A, 0.284A avg, 4.08W",Subject: "da sampling at most 1000ml, wcr at most 1000ml" @13:45:20.40
SC.reconfigure :tight @13:45:20.45 SC.seek 3 @13:45:24.02 SC.configure :limp @13:45:24.23 Elbow.reconfigure :tight @13:45:24.28 Elbow.seek 3 @13:45:25.53 upsync @13:45:26.25
Forearm.reconfigure :tight @13:45:26.30 Forearm.seek 3 + 450 counts @13:45:27.29 SE.reconfigure :pick @13:45:27.34 SE.seek :beamBreak @13:45:28.58 SE.configure :default @13:45:28.73 Elbow.seek 3 @13:45:28.90 Forearm.seek 3 + 300 counts @13:45:29.20 Forearm.reconfigure :loose @13:45:29.24 Hand.close @13:45:29.86 SE.seek :down @13:45:30.05
Forearm.seek :retract @13:45:30.72 SE.stop @13:45:31.05
Elbow.seek CC @13:45:32.59 Elbow.reconfigure :loose @13:45:32.68 Forearm.reconfigure :tight @13:45:32.72 Forearm.seek CC @13:45:34.00 CC.seek :closed @13:45:38.51 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.29 @13:45:38.52 Hand.open @13:45:39.16 Forearm.reconfigure :loose @13:45:39.20 Forearm.seek :retract @13:45:40.18 collectSample 1000ml w/bubble point of 24psi @13:45:40.19 Priming 7ml @13:45:40.20 Intake.select :close @13:45:40.21 Exhaust.select :close @13:45:40.22 SS.hold @13:45:40.42 Calibrating pressure and temperature sensors @13:45:40.45 CTV.dial :air @13:45:40.46 SSV.dial :spit,avoiding: :exhaust @13:45:40.48 SS.reconfigure :fast @13:45:40.52 SS.seek 5ml @13:45:43.55 SSV.dial CBV @13:45:43.58
CBV.dial SSV @13:45:44.87 delay 3.8 seconds @13:45:48.78 SSV.dial :spit @13:45:50.11 SS.seek 0ml @13:45:52.87 SSV.dial CBV @13:45:54.16 CTV.dial :intake @13:45:54.94 SSV.dial CBV @13:45:54.96
CBV.dial SSV @13:45:54.98 Allowing 40s for 7.0ml of remaining 7.0ml @13:45:54.99 Intake.select :open @13:45:57.14 Intake @15.1psia, vacuum from 9.6 to 12.6psi @13:45:57.16 SS.reconfigure :inhaling 2.20ml/s @13:45:57.20 SS.seek 7ml @13:46:03.79 Stroke completed -- waiting for pressure to equalize ... @13:46:03.80 SS.reconfigure :exhale @13:46:03.84 SS.hold @13:46:06.74 Intake.select :close @13:46:06.88 SSV.dial :exhaust @13:46:07.62 Exhaust.select :open @13:46:07.73 SS.seek 0ml @13:46:11.90 Exhaust.select :close @13:46:12.00 Sampling 1000.0ml @13:46:12.01 Intake.select :close @13:46:12.02 Exhaust.select :close @13:46:12.03 SS.reconfigure :default @13:46:12.07 SS.hold @13:46:12.26 CTV.dial :intake @13:46:12.27 SSV.dial CBV @13:46:12.29
CBV.dial SSV @13:46:13.06 Allowing 130s for 25.0ml of remaining 1000.0ml @13:46:13.07 Intake.select :open @13:46:15.22 Intake @15.5psia, vacuum from 10.0 to 13.0psi @13:46:15.23 SS.reconfigure :inhaling 2.20ml/s @13:46:15.28 SS.seek 25ml @13:46:24.24 Vacuum too high -- waiting up to 40 seconds @13:46:24.25 SS.reconfigure :exhale @13:46:24.29 SS.hold @13:46:28.42 Intake @14.4psia, vacuum from 8.9 to 11.9psi @13:46:28.43 SS.reconfigure :inhaling 1.98ml/s @13:46:28.48 SS.seek 25ml @13:46:35.95 Stroke completed -- waiting for pressure to equalize ... @13:46:35.96 SS.reconfigure :exhale @13:46:36.00 SS.hold @13:46:44.46 Intake.select :close @13:46:44.60 SSV.dial :exhaust @13:46:45.36 Exhaust.select :open @13:46:45.46 SS.seek 0ml @13:46:54.23 Exhaust.select :close @13:46:54.33 SSV.dial CBV @13:46:54.36
CBV.dial SSV @13:46:55.10 Allowing 130s for 25.0ml of remaining 975.0ml @13:46:55.11 Intake.select :open @13:46:57.27 Intake @15.9psia, vacuum from 10.0 to 13.0psi @13:46:57.29 SS.reconfigure :inhaling 1.98ml/s @13:46:57.33 SS.seek 25ml @13:47:05.89 Vacuum too high -- waiting up to 40 seconds @13:47:05.90 SS.reconfigure :exhale @13:47:05.94 SS.hold @13:47:09.72 Intake @14.5psia, vacuum from 9.0 to 12.0psi @13:47:09.74 SS.reconfigure :inhaling 1.79ml/s @13:47:09.78 SS.seek 25ml @13:47:18.69 Stroke completed -- waiting for pressure to equalize ... @13:47:18.70 SS.reconfigure :exhale @13:47:18.74 SS.hold @13:47:28.43 Intake.select :close @13:47:28.57 SSV.dial :exhaust @13:47:29.31 Exhaust.select :open @13:47:29.41 SS.seek 0ml @13:47:38.21 Exhaust.select :close @13:47:38.31 SSV.dial CBV @13:47:38.34
CBV.dial SSV @13:47:39.09 Allowing 130s for 25.0ml of remaining 950.0ml @13:47:39.10 Intake.select :open @13:47:41.25 Intake @16.0psia, vacuum from 10.0 to 13.0psi @13:47:41.27 SS.reconfigure :inhaling 1.79ml/s @13:47:41.31 SS.seek 25ml @13:47:49.02 Vacuum too high -- waiting up to 40 seconds @13:47:49.03 SS.reconfigure :exhale @13:47:49.07 SS.hold @13:47:51.79 Intake @14.4psia, vacuum from 8.9 to 11.9psi @13:47:51.80 SS.reconfigure :inhaling 1.61ml/s @13:47:51.85 SS.seek 25ml @13:48:03.01 Stroke completed -- waiting for pressure to equalize ... @13:48:03.02 SS.reconfigure :exhale @13:48:03.06 SS.hold @13:48:14.10 Intake.select :close @13:48:14.23 SSV.dial :exhaust @13:48:14.99 Exhaust.select :open @13:48:15.10 SS.seek 0ml @13:48:23.93 Exhaust.select :close @13:48:24.03 SSV.dial CBV @13:48:24.05
CBV.dial SSV @13:48:24.82 Allowing 130s for 25.0ml of remaining 925.0ml @13:48:24.83 Intake.select :open @13:48:27.83 Intake @15.9psia, vacuum from 10.0 to 13.0psi @13:48:27.85 SS.reconfigure :inhaling 1.61ml/s @13:48:27.89 SS.seek 25ml @13:48:38.05 Vacuum too high -- waiting up to 40 seconds @13:48:38.06 SS.reconfigure :exhale @13:48:38.10 SS.hold @13:48:41.47 Intake @14.4psia, vacuum from 8.9 to 11.9psi @13:48:41.48 SS.reconfigure :inhaling 1.45ml/s @13:48:41.53 SS.seek 25ml @13:48:51.47 Stroke completed -- waiting for pressure to equalize ... @13:48:51.48 SS.reconfigure :exhale @13:48:51.53 SS.hold @13:49:01.29 Intake.select :close @13:49:01.42 SSV.dial :exhaust @13:49:02.19 Exhaust.select :open @13:49:02.29 SS.seek 0ml @13:49:11.08 Exhaust.select :close @13:49:11.19 SSV.dial CBV @13:49:11.23
CBV.dial SSV @13:49:11.96 Allowing 130s for 25.0ml of remaining 900.0ml @13:49:11.97 Intake.select :open @13:49:14.12 Intake @15.9psia, vacuum from 10.0 to 13.0psi @13:49:14.14 SS.reconfigure :inhaling 1.45ml/s @13:49:14.18 SS.seek 25ml @13:49:25.98 Vacuum too high -- waiting up to 40 seconds @13:49:25.99 SS.reconfigure :exhale @13:49:26.03 SS.hold @13:49:29.68 Intake @14.6psia, vacuum from 9.1 to 12.1psi @13:49:29.70 SS.reconfigure :inhaling 1.30ml/s @13:49:29.74 SS.seek 25ml @13:49:39.85 Stroke completed -- waiting for pressure to equalize ... @13:49:39.86 SS.reconfigure :exhale @13:49:39.90 SS.hold @13:49:48.71 Intake.select :close @13:49:48.85 SSV.dial :exhaust @13:49:49.60 Exhaust.select :open @13:49:49.71 SS.seek 0ml @13:49:58.46 Exhaust.select :close @13:49:58.56 SSV.dial CBV @13:49:58.58
CBV.dial SSV @13:49:59.35 Allowing 130s for 25.0ml of remaining 875.0ml @13:49:59.36 Intake.select :open @13:50:01.51 Intake @15.9psia, vacuum from 10.0 to 13.0psi @13:50:01.53 SS.reconfigure :inhaling 1.30ml/s @13:50:01.57 SS.seek 25ml @13:50:15.26 Vacuum too high -- waiting up to 40 seconds @13:50:15.27 SS.reconfigure :exhale @13:50:15.31 SS.hold @13:50:19.52 Intake @14.8psia, vacuum from 9.3 to 12.3psi @13:50:19.54 SS.reconfigure :inhaling 1.17ml/s @13:50:19.58 SS.seek 25ml @13:50:29.36 Stroke completed -- waiting for pressure to equalize ... @13:50:29.37 SS.reconfigure :exhale @13:50:29.41 SS.hold @13:50:37.94 Intake.select :close @13:50:38.07 SSV.dial :exhaust @13:50:38.83 Exhaust.select :open @13:50:38.94 SS.seek 0ml @13:50:47.68 Exhaust.select :close @13:50:47.78 SSV.dial CBV @13:50:47.80
CBV.dial SSV @13:50:48.55 Allowing 130s for 25.0ml of remaining 850.0ml @13:50:48.56 Intake.select :open @13:50:50.71 Intake @15.8psia, vacuum from 10.0 to 13.0psi @13:50:50.73 SS.reconfigure :inhaling 1.17ml/s @13:50:50.77 SS.seek 25ml @13:51:08.82 Vacuum too high -- waiting up to 40 seconds @13:51:08.83 SS.reconfigure :exhale @13:51:08.87 SS.hold @13:51:13.91 Intake @14.8psia, vacuum from 9.3 to 12.3psi @13:51:13.92 SS.reconfigure :inhaling 1.06ml/s @13:51:13.97 SS.seek 25ml @13:51:21.54 Stroke completed -- waiting for pressure to equalize ... @13:51:21.55 SS.reconfigure :exhale @13:51:21.59 SS.hold @13:51:29.04 Intake.select :close @13:51:29.17 SSV.dial :exhaust @13:51:29.91 Exhaust.select :open @13:51:30.02 SS.seek 0ml @13:51:38.75 Exhaust.select :close @13:51:38.86 SSV.dial CBV @13:51:38.91
CBV.dial SSV @13:51:39.61 Allowing 130s for 25.0ml of remaining 825.0ml @13:51:39.62 Intake.select :open @13:51:41.78 Intake @15.8psia, vacuum from 10.0 to 13.0psi @13:51:41.79 SS.reconfigure :inhaling 1.06ml/s @13:51:41.84 SS.seek 25ml @13:52:05.67 Vacuum too high -- waiting up to 40 seconds @13:52:05.68 SS.reconfigure :exhale @13:52:05.72 SS.hold @13:52:11.34 Intake @14.9psia, vacuum from 9.4 to 12.4psi @13:52:11.36 SS.reconfigure :inhaling 0.95ml/s @13:52:11.40 SS.seek 25ml @13:52:14.74 Stroke completed -- waiting for pressure to equalize ... @13:52:14.75 SS.reconfigure :exhale @13:52:14.79 SS.hold @13:52:21.21 Intake.select :close @13:52:21.36 SSV.dial :exhaust @13:52:22.10 Exhaust.select :open @13:52:22.20 SS.seek 0ml @13:52:30.99 Exhaust.select :close @13:52:31.09 SSV.dial CBV @13:52:31.11
CBV.dial SSV @13:52:31.86 Allowing 130s for 25.0ml of remaining 800.0ml @13:52:31.87 Intake.select :open @13:52:34.02 Intake @15.9psia, vacuum from 10.0 to 13.0psi @13:52:34.04 SS.reconfigure :inhaling 0.95ml/s @13:52:34.08 SS.seek 25ml @13:52:58.79 Vacuum too high -- waiting up to 40 seconds @13:52:58.80 SS.reconfigure :exhale @13:52:58.84 SS.hold @13:53:03.69 Intake @15.0psia, vacuum from 9.5 to 12.5psi @13:53:03.71 SS.reconfigure :inhaling 0.87ml/s @13:53:03.75 SS.seek 25ml @13:53:08.47 Stroke completed -- waiting for pressure to equalize ... @13:53:08.48 SS.reconfigure :exhale @13:53:08.52 SS.hold @13:53:15.89 Intake.select :close @13:53:16.02 SSV.dial :exhaust @13:53:16.78 Exhaust.select :open @13:53:16.89 SS.seek 0ml @13:53:25.66 Exhaust.select :close @13:53:25.76 SSV.dial CBV @13:53:25.78
CBV.dial SSV @13:53:26.55 Allowing 130s for 25.0ml of remaining 775.0ml @13:53:26.56 Intake.select :open @13:53:28.71 Intake @15.7psia, vacuum from 10.0 to 13.0psi @13:53:28.73 SS.reconfigure :inhaling 0.87ml/s @13:53:28.77 SS.seek 25ml @13:54:00.22 Stroke completed -- waiting for pressure to equalize ... @13:54:00.23 SS.reconfigure :exhale @13:54:00.27 SS.hold @13:54:11.78 Intake.select :close @13:54:11.91 SSV.dial :exhaust @13:54:12.67 Exhaust.select :open @13:54:12.78 SS.seek 0ml @13:54:21.48 Exhaust.select :close @13:54:21.59 SSV.dial CBV @13:54:21.64
CBV.dial SSV @13:54:22.36 Allowing 130s for 25.0ml of remaining 750.0ml @13:54:22.37 Intake.select :open @13:54:24.52 Intake @15.8psia, vacuum from 10.0 to 13.0psi @13:54:24.54 SS.reconfigure :inhaling 0.87ml/s @13:54:24.58 SS.seek 25ml @13:54:52.57 Vacuum too high -- waiting up to 40 seconds @13:54:52.58 SS.reconfigure :exhale @13:54:52.62 SS.hold @13:54:58.26 Intake @15.1psia, vacuum from 9.6 to 12.6psi @13:54:58.27 SS.reconfigure :inhaling 0.78ml/s @13:54:58.31 SS.seek 25ml @13:55:02.15 Stroke completed -- waiting for pressure to equalize ... @13:55:02.16 SS.reconfigure :exhale @13:55:02.20 SS.hold @13:55:08.60 Intake.select :close @13:55:08.74 SSV.dial :exhaust @13:55:09.50 Exhaust.select :open @13:55:09.61 SS.seek 0ml @13:55:18.33 Exhaust.select :close @13:55:18.43 SSV.dial CBV @13:55:18.45
CBV.dial SSV @13:55:19.22 Allowing 130s for 25.0ml of remaining 725.0ml @13:55:19.23 Intake.select :open @13:55:21.38 Intake @15.8psia, vacuum from 10.0 to 13.0psi @13:55:21.40 SS.reconfigure :inhaling 0.78ml/s @13:55:21.44 SS.seek 25ml @13:55:56.29 Stroke completed -- waiting for pressure to equalize ... @13:55:56.30 SS.reconfigure :exhale @13:55:56.35 SS.hold @13:56:07.37 Intake.select :close @13:56:07.51 SSV.dial :exhaust @13:56:08.26 Exhaust.select :open @13:56:08.37 SS.seek 0ml @13:56:17.11 Exhaust.select :close @13:56:17.21 SSV.dial CBV @13:56:17.23
CBV.dial SSV @13:56:17.98 Allowing 130s for 25.0ml of remaining 700.0ml @13:56:17.99 Intake.select :open @13:56:20.14 Intake @15.8psia, vacuum from 10.0 to 13.0psi @13:56:20.16 SS.reconfigure :inhaling 0.78ml/s @13:56:20.20 SS.seek 25ml @13:56:54.75 Stroke completed -- waiting for pressure to equalize ... @13:56:54.76 SS.reconfigure :exhale @13:56:54.80 SS.hold @13:57:07.34 Intake.select :close @13:57:07.47 SSV.dial :exhaust @13:57:08.22 Exhaust.select :open @13:57:08.32 SS.seek 0ml @13:57:17.09 Exhaust.select :close @13:57:17.19 SSV.dial CBV @13:57:17.21
CBV.dial SSV @13:57:17.97 Allowing 130s for 25.0ml of remaining 675.0ml @13:57:17.98 Intake.select :open @13:57:20.13 Intake @15.7psia, vacuum from 10.0 to 13.0psi @13:57:20.14 SS.reconfigure :inhaling 0.78ml/s @13:57:20.19 SS.seek 25ml @13:57:54.93 Stroke completed -- waiting for pressure to equalize ... @13:57:54.94 SS.reconfigure :exhale @13:57:54.98 SS.hold @13:58:08.05 Intake.select :close @13:58:08.19 SSV.dial :exhaust @13:58:08.93 Exhaust.select :open @13:58:09.04 SS.seek 0ml @13:58:17.84 Exhaust.select :close @13:58:17.94 SSV.dial CBV @13:58:17.96
CBV.dial SSV @13:58:18.72 Allowing 130s for 25.0ml of remaining 650.0ml @13:58:18.73 Intake.select :open @13:58:20.88 Intake @15.7psia, vacuum from 10.0 to 13.0psi @13:58:20.89 SS.reconfigure :inhaling 0.78ml/s @13:58:20.94 SS.seek 25ml @13:58:55.60 Vacuum too high -- waiting up to 40 seconds @13:58:55.61 SS.reconfigure :exhale @13:58:55.65 SS.hold @13:59:02.14 Intake @14.8psia, vacuum from 9.3 to 12.3psi @13:59:02.16 SS.reconfigure :inhaling 0.70ml/s @13:59:02.20 SS.seek 25ml @13:59:02.25 Stroke completed -- waiting for pressure to equalize ... SS.reconfigure :exhale @13:59:02.29 SS.hold @13:59:10.74 Intake.select :close @13:59:10.88 SSV.dial :exhaust @13:59:11.64 Exhaust.select :open @13:59:11.74 SS.seek 0ml @13:59:20.74 Exhaust.select :close @13:59:20.85 SSV.dial CBV @13:59:20.89
CBV.dial SSV @13:59:21.62 Allowing 130s for 25.0ml of remaining 625.0ml @13:59:21.63 Intake.select :open @13:59:23.78 Intake @15.6psia, vacuum from 10.0 to 13.0psi @13:59:23.80 SS.reconfigure :inhaling 0.70ml/s @13:59:23.84 SS.seek 25ml @14:00:01.16 Stroke completed -- waiting for pressure to equalize ... @14:00:01.17 SS.reconfigure :exhale @14:00:01.21 SS.hold @14:00:15.45 Intake.select :close @14:00:15.59 SSV.dial :exhaust @14:00:16.34 Exhaust.select :open @14:00:16.44 SS.seek 0ml @14:00:25.27 Exhaust.select :close @14:00:25.38 SSV.dial CBV @14:00:25.43
CBV.dial SSV @14:00:26.15 Allowing 130s for 25.0ml of remaining 600.0ml @14:00:26.16 Intake.select :open @14:00:28.31 Intake @15.6psia, vacuum from 10.0 to 13.0psi @14:00:28.33 SS.reconfigure :inhaling 0.70ml/s @14:00:28.37 SS.seek 25ml @14:01:06.27 Stroke completed -- waiting for pressure to equalize ... @14:01:06.28 SS.reconfigure :exhale @14:01:06.32 SS.hold @14:01:22.52 Intake.select :close @14:01:22.66 SSV.dial :exhaust @14:01:23.41 Exhaust.select :open @14:01:23.52 SS.seek 0ml @14:01:32.54 Exhaust.select :close @14:01:32.64 SSV.dial CBV @14:01:32.67
CBV.dial SSV @14:01:33.42 Allowing 130s for 25.0ml of remaining 575.0ml @14:01:33.43 Intake.select :open @14:01:35.58 Intake @15.5psia, vacuum from 10.0 to 13.0psi @14:01:35.59 SS.reconfigure :inhaling 0.70ml/s @14:01:35.64 SS.seek 25ml @14:02:13.02 Stroke completed -- waiting for pressure to equalize ... @14:02:13.03 SS.reconfigure :exhale @14:02:13.08 SS.hold @14:02:31.37 Intake.select :close @14:02:31.51 SSV.dial :exhaust @14:02:32.26 Exhaust.select :open @14:02:32.36 SS.seek 0ml @14:02:41.15 Exhaust.select :close @14:02:41.25 SSV.dial CBV @14:02:41.27
CBV.dial SSV @14:02:42.02 Allowing 130s for 25.0ml of remaining 550.0ml @14:02:42.03 Intake.select :open @14:02:44.19 Intake @15.5psia, vacuum from 10.0 to 13.0psi @14:02:44.20 SS.reconfigure :inhaling 0.70ml/s @14:02:44.24 SS.seek 25ml @14:03:22.39 Stroke completed -- waiting for pressure to equalize ... @14:03:22.40 SS.reconfigure :exhale @14:03:22.45 SS.hold @14:03:40.69 Intake.select :close @14:03:40.82 SSV.dial :exhaust @14:03:41.57 Exhaust.select :open @14:03:41.68 SS.seek 0ml @14:03:50.49 Exhaust.select :close @14:03:50.59 SSV.dial CBV @14:03:50.61
CBV.dial SSV @14:03:51.37 Allowing 130s for 25.0ml of remaining 525.0ml @14:03:51.38 Intake.select :open @14:03:53.53 Intake @15.6psia, vacuum from 10.0 to 13.0psi @14:03:53.54 SS.reconfigure :inhaling 0.70ml/s @14:03:53.59 SS.seek 25ml @14:04:19.40 Vacuum too high -- waiting up to 40 seconds @14:04:19.41 SS.reconfigure :exhale @14:04:19.45 SS.hold @14:04:27.97 Intake @15.3psia, vacuum from 9.8 to 12.8psi @14:04:27.99 SS.reconfigure :inhaling 0.63ml/s @14:04:28.03 SS.seek 25ml @14:04:42.52 Stroke completed -- waiting for pressure to equalize ... @14:04:42.53 SS.reconfigure :exhale @14:04:42.57 SS.hold @14:04:57.14 Intake.select :close @14:04:57.28 SSV.dial :exhaust @14:04:58.02 Exhaust.select :open @14:04:58.13 SS.seek 0ml @14:05:06.94 Exhaust.select :close @14:05:07.04 SSV.dial CBV @14:05:07.08
CBV.dial SSV @14:05:07.83 Allowing 130s for 25.0ml of remaining 500.0ml @14:05:07.84 Intake.select :open @14:05:09.99 Intake @15.6psia, vacuum from 10.0 to 13.0psi @14:05:10.00 SS.reconfigure :inhaling 0.63ml/s @14:05:10.05 SS.seek 25ml @14:05:46.56 Vacuum too high -- waiting up to 40 seconds @14:05:46.57 SS.reconfigure :exhale @14:05:46.61 SS.hold @14:05:55.18 Intake @14.9psia, vacuum from 9.4 to 12.4psi @14:05:55.19 SS.reconfigure :inhaling 0.57ml/s @14:05:55.24 SS.seek 25ml @14:06:01.69 Stroke completed -- waiting for pressure to equalize ... @14:06:01.70 SS.reconfigure :exhale @14:06:01.74 SS.hold @14:06:14.27 Intake.select :close @14:06:14.41 SSV.dial :exhaust @14:06:15.14 Exhaust.select :open @14:06:15.25 SS.seek 0ml @14:06:24.08 Exhaust.select :close @14:06:24.18 SSV.dial CBV @14:06:24.20
CBV.dial SSV @14:06:24.96 Allowing 130s for 25.0ml of remaining 475.0ml @14:06:24.97 Intake.select :open @14:06:27.12 Intake @15.6psia, vacuum from 10.0 to 13.0psi @14:06:27.14 SS.reconfigure :inhaling 0.57ml/s @14:06:27.18 SS.seek 25ml @14:07:13.27 Stroke completed -- waiting for pressure to equalize ... @14:07:13.28 SS.reconfigure :exhale @14:07:13.32 SS.hold @14:07:31.62 Intake.select :close @14:07:31.76 SSV.dial :exhaust @14:07:32.52 Exhaust.select :open @14:07:32.62 SS.seek 0ml @14:07:41.66 Exhaust.select :close @14:07:41.76 SSV.dial CBV @14:07:41.78
CBV.dial SSV @14:07:42.55 Allowing 130s for 25.0ml of remaining 450.0ml @14:07:42.56 Intake.select :open @14:07:44.71 Intake @15.5psia, vacuum from 10.0 to 13.0psi @14:07:44.73 SS.reconfigure :inhaling 0.57ml/s @14:07:45.24 SS.seek 25ml @14:08:30.72 Stroke completed -- waiting for pressure to equalize ... @14:08:30.73 SS.reconfigure :exhale @14:08:30.77 SS.hold @14:08:53.07 Intake.select :close @14:08:53.21 SSV.dial :exhaust @14:08:53.97 Exhaust.select :open @14:08:54.07 SS.seek 0ml @14:09:03.11 Exhaust.select :close @14:09:03.21 SSV.dial CBV @14:09:03.23
CBV.dial SSV @14:09:03.99 Allowing 130s for 25.0ml of remaining 425.0ml @14:09:04.00 Intake.select :open @14:09:06.15 Intake @15.4psia, vacuum from 9.9 to 12.9psi @14:09:06.17 SS.reconfigure :inhaling 0.57ml/s @14:09:06.21 SS.seek 25ml @14:09:51.87 Stroke completed -- waiting for pressure to equalize ... @14:09:51.88 SS.reconfigure :exhale @14:09:51.92 SS.hold @14:10:14.22 Intake.select :close @14:10:14.35 SSV.dial :exhaust @14:10:15.09 Exhaust.select :open @14:10:15.20 SS.seek 0ml @14:10:23.97 Exhaust.select :close @14:10:24.07 SSV.dial CBV @14:10:24.09
CBV.dial SSV @14:10:24.84 Allowing 130s for 25.0ml of remaining 400.0ml @14:10:24.85 Intake.select :open @14:10:27.01 Intake @15.5psia, vacuum from 10.0 to 13.0psi @14:10:27.03 SS.reconfigure :inhaling 0.57ml/s @14:10:27.07 SS.seek 25ml @14:11:01.24 Vacuum too high -- waiting up to 40 seconds @14:11:01.25 SS.reconfigure :exhale @14:11:01.29 SS.hold @14:11:12.00 Intake @15.5psia, vacuum from 10.0 to 13.0psi @14:11:12.02 SS.reconfigure :inhaling 0.51ml/s @14:11:12.06 SS.seek 25ml @14:11:25.99 Stroke completed -- waiting for pressure to equalize ... @14:11:26.00 SS.reconfigure :exhale @14:11:26.04 SS.hold @14:11:42.22 Intake.select :close @14:11:42.36 SSV.dial :exhaust @14:11:43.11 Exhaust.select :open @14:11:43.21 SS.seek 0ml @14:11:51.99 Exhaust.select :close @14:11:52.10 SSV.dial CBV @14:11:52.12
CBV.dial SSV @14:11:52.87 Allowing 130s for 25.0ml of remaining 375.0ml @14:11:52.88 Intake.select :open @14:11:55.04 Intake @15.5psia, vacuum from 10.0 to 13.0psi @14:11:55.05 SS.reconfigure :inhaling 0.51ml/s @14:11:55.09 SS.seek 25ml @14:12:46.34 Stroke completed -- waiting for pressure to equalize ... @14:12:46.35 SS.reconfigure :exhale @14:12:46.39 SS.hold @14:13:10.85 Intake.select :close @14:13:10.99 SSV.dial :exhaust @14:13:11.73 Exhaust.select :open @14:13:11.84 SS.seek 0ml @14:13:20.63 Exhaust.select :close @14:13:20.74 SSV.dial CBV @14:13:20.79
CBV.dial SSV @14:13:21.50 Allowing 130s for 25.0ml of remaining 350.0ml @14:13:21.51 Intake.select :open @14:13:23.66 Intake @15.5psia, vacuum from 10.0 to 13.0psi @14:13:23.67 SS.reconfigure :inhaling 0.51ml/s @14:13:23.72 SS.seek 25ml @14:14:02.58 Vacuum too high -- waiting up to 40 seconds @14:14:02.59 SS.reconfigure :exhale @14:14:02.63 SS.hold @14:14:13.67 Intake @15.2psia, vacuum from 9.7 to 12.7psi @14:14:13.68 SS.reconfigure :inhaling 0.47ml/s @14:14:13.73 SS.seek 25ml @14:14:27.57 Stroke completed -- waiting for pressure to equalize ... @14:14:27.58 SS.reconfigure :exhale @14:14:27.62 SS.hold @14:14:45.92 Intake.select :close @14:14:46.05 SSV.dial :exhaust @14:14:46.81 Exhaust.select :open @14:14:46.91 SS.seek 0ml @14:14:55.88 Exhaust.select :close @14:14:55.98 SSV.dial CBV @14:14:56.01
CBV.dial SSV @14:14:56.77 Allowing 130s for 25.0ml of remaining 325.0ml @14:14:56.78 Intake.select :open @14:14:58.94 Intake @15.5psia, vacuum from 10.0 to 13.0psi @14:14:58.95 SS.reconfigure :inhaling 0.47ml/s @14:14:59.00 SS.seek 25ml @14:15:54.55 Stroke completed -- waiting for pressure to equalize ... @14:15:54.56 SS.reconfigure :exhale @14:15:54.60 SS.hold @14:16:19.85 Intake.select :close @14:16:19.99 SSV.dial :exhaust @14:16:20.74 Exhaust.select :open @14:16:20.85 SS.seek 0ml @14:16:29.73 Exhaust.select :close @14:16:29.83 SSV.dial CBV @14:16:29.86
CBV.dial SSV @14:16:30.62 Allowing 130s for 25.0ml of remaining 300.0ml @14:16:30.63 Intake.select :open @14:16:32.78 Intake @15.7psia, vacuum from 10.0 to 13.0psi @14:16:32.79 SS.reconfigure :inhaling 0.47ml/s @14:16:32.84 SS.seek 25ml @14:17:08.98 Vacuum too high -- waiting up to 40 seconds @14:17:08.99 SS.reconfigure :exhale @14:17:09.03 SS.hold @14:17:18.32 Intake @15.1psia, vacuum from 9.6 to 12.6psi @14:17:18.33 SS.reconfigure :inhaling 0.43ml/s @14:17:18.38 SS.seek 25ml @14:17:40.74 Stroke completed -- waiting for pressure to equalize ... @14:17:40.75 SS.reconfigure :exhale @14:17:40.79 SS.hold @14:18:01.18 Intake.select :close @14:18:01.32 SSV.dial :exhaust @14:18:02.12 Exhaust.select :open @14:18:02.23 SS.seek 0ml @14:18:11.08 Exhaust.select :close @14:18:11.18 SSV.dial CBV @14:18:11.20
CBV.dial SSV @14:18:11.96 Allowing 130s for 25.0ml of remaining 275.0ml @14:18:11.97 Intake.select :open @14:18:14.12 Intake @15.6psia, vacuum from 10.0 to 13.0psi @14:18:14.14 SS.reconfigure :inhaling 0.43ml/s @14:18:14.18 SS.seek 25ml @14:19:09.11 Vacuum too high -- waiting up to 40 seconds @14:19:09.12 SS.reconfigure :exhale @14:19:09.16 SS.hold @14:19:21.69 Intake @15.3psia, vacuum from 9.8 to 12.8psi @14:19:21.71 SS.reconfigure :inhaling 0.38ml/s @14:19:21.75 SS.seek 25ml @14:19:30.13 Stroke completed -- waiting for pressure to equalize ... @14:19:30.14 SS.reconfigure :exhale @14:19:30.18 SS.hold @14:19:44.72 Intake.select :close @14:19:44.86 SSV.dial :exhaust @14:19:45.60 Exhaust.select :open @14:19:45.71 SS.seek 0ml @14:19:54.50 Exhaust.select :close @14:19:54.60 SSV.dial CBV @14:19:54.63
CBV.dial SSV @14:19:55.38 Allowing 130s for 25.0ml of remaining 250.0ml @14:19:55.39 Intake.select :open @14:19:57.54 Intake @15.6psia, vacuum from 10.0 to 13.0psi @14:19:57.56 SS.reconfigure :inhaling 0.38ml/s @14:19:57.60 SS.seek 25ml @14:21:08.28 Stroke completed -- waiting for pressure to equalize ... @14:21:08.29 SS.reconfigure :exhale @14:21:08.33 SS.hold @14:21:32.67 Intake.select :close @14:21:32.81 SSV.dial :exhaust @14:21:33.55 Exhaust.select :open @14:21:33.65 SS.seek 0ml @14:21:42.41 Exhaust.select :close @14:21:42.51 SSV.dial CBV @14:21:42.53
CBV.dial SSV @14:21:43.28 Allowing 130s for 25.0ml of remaining 225.0ml @14:21:43.29 Intake.select :open @14:21:45.44 Intake @15.7psia, vacuum from 10.0 to 13.0psi @14:21:45.46 SS.reconfigure :inhaling 0.38ml/s @14:21:45.50 SS.seek 25ml @14:22:56.97 Stroke completed -- waiting for pressure to equalize ... @14:22:56.98 SS.reconfigure :exhale @14:22:57.02 SS.hold @14:23:23.42 Intake.select :close @14:23:23.56 SSV.dial :exhaust @14:23:24.30 Exhaust.select :open @14:23:24.41 SS.seek 0ml @14:23:33.28 Exhaust.select :close @14:23:33.38 SSV.dial CBV @14:23:33.40
CBV.dial SSV @14:23:34.16 Allowing 130s for 25.0ml of remaining 200.0ml @14:23:34.17 Intake.select :open @14:23:36.32 Intake @15.6psia, vacuum from 10.0 to 13.0psi @14:23:36.34 SS.reconfigure :inhaling 0.38ml/s @14:23:36.38 SS.seek 25ml @14:24:46.99 Stroke completed -- waiting for pressure to equalize ... @14:24:47.00 SS.reconfigure :exhale @14:24:47.04 SS.hold @14:25:15.48 Intake.select :close @14:25:15.62 SSV.dial :exhaust @14:25:16.37 Exhaust.select :open @14:25:16.48 SS.seek 0ml @14:25:25.49 Exhaust.select :close @14:25:25.60 SSV.dial CBV @14:25:25.62
CBV.dial SSV @14:25:26.39 Allowing 130s for 25.0ml of remaining 175.0ml @14:25:26.40 Intake.select :open @14:25:28.55 Intake @15.6psia, vacuum from 10.0 to 13.0psi @14:25:28.57 SS.reconfigure :inhaling 0.38ml/s @14:25:28.61 SS.seek 25ml @14:26:40.30 Stroke completed -- waiting for pressure to equalize ... @14:26:40.31 SS.reconfigure :exhale @14:26:40.36 SS.hold @14:27:10.83 Intake.select :close @14:27:10.97 SSV.dial :exhaust @14:27:11.73 Exhaust.select :open @14:27:11.83 SS.seek 0ml @14:27:20.63 Exhaust.select :close @14:27:20.74 SSV.dial CBV @14:27:20.79
CBV.dial SSV @14:27:21.51 Allowing 130s for 25.0ml of remaining 150.0ml @14:27:21.52 Intake.select :open @14:27:23.67 Intake @15.6psia, vacuum from 10.0 to 13.0psi @14:27:23.69 SS.reconfigure :inhaling 0.38ml/s @14:27:23.73 SS.seek 25ml @14:28:33.03 Vacuum too high -- waiting up to 40 seconds @14:28:33.04 SS.reconfigure :exhale @14:28:33.08 SS.hold @14:28:49.29 Intake @15.5psia, vacuum from 10.0 to 13.0psi @14:28:49.30 SS.reconfigure :inhaling 0.34ml/s @14:28:49.35 SS.seek 25ml @14:28:52.43 Stroke completed -- waiting for pressure to equalize ... @14:28:52.44 SS.reconfigure :exhale @14:28:52.48 SS.hold @14:29:09.13 Intake.select :close @14:29:09.27 SSV.dial :exhaust @14:29:10.02 Exhaust.select :open @14:29:10.13 SS.seek 0ml @14:29:18.99 Exhaust.select :close @14:29:19.09 SSV.dial CBV @14:29:19.11
CBV.dial SSV @14:29:19.87 Allowing 130s for 25.0ml of remaining 125.0ml @14:29:19.88 Intake.select :open @14:29:22.03 Intake @15.5psia, vacuum from 10.0 to 13.0psi @14:29:22.05 SS.reconfigure :inhaling 0.34ml/s @14:29:22.09 SS.seek 25ml @14:30:46.93 Stroke completed -- waiting for pressure to equalize ... @14:30:46.94 SS.reconfigure :exhale @14:30:46.98 SS.hold @14:31:17.55 Intake.select :close @14:31:17.69 SSV.dial :exhaust @14:31:18.43 Exhaust.select :open @14:31:18.53 SS.seek 0ml @14:31:27.36 Exhaust.select :close @14:31:27.47 SSV.dial CBV @14:31:27.50
CBV.dial SSV @14:31:28.22 Allowing 130s for 25.0ml of remaining 100.0ml @14:31:28.23 Intake.select :open @14:31:30.39 Intake @15.6psia, vacuum from 10.0 to 13.0psi @14:31:30.41 SS.reconfigure :inhaling 0.34ml/s @14:31:30.45 SS.seek 25ml @14:32:55.27 Stroke completed -- waiting for pressure to equalize ... @14:32:55.28 SS.reconfigure :exhale @14:32:55.32 SS.hold @14:33:27.94 Intake.select :close @14:33:28.08 SSV.dial :exhaust @14:33:28.82 Exhaust.select :open @14:33:28.93 SS.seek 0ml @14:33:37.97 Exhaust.select :close @14:33:38.07 SSV.dial CBV @14:33:38.09
CBV.dial SSV @14:33:38.85 Allowing 130s for 25.0ml of remaining 75.0ml @14:33:38.86 Intake.select :open @14:33:41.01 Intake @15.6psia, vacuum from 10.0 to 13.0psi @14:33:41.02 SS.reconfigure :inhaling 0.34ml/s @14:33:41.07 SS.seek 25ml @14:35:03.06 Vacuum too high -- waiting up to 40 seconds @14:35:03.07 SS.reconfigure :exhale @14:35:03.11 SS.hold @14:35:21.33 Intake @15.5psia, vacuum from 10.0 to 13.0psi @14:35:21.35 SS.reconfigure :inhaling 0.31ml/s @14:35:21.39 SS.seek 25ml @14:35:22.51 Stroke completed -- waiting for pressure to equalize ... @14:35:22.52 SS.reconfigure :exhale @14:35:22.57 SS.hold @14:35:40.87 Intake.select :close @14:35:41.00 SSV.dial :exhaust @14:35:41.77 Exhaust.select :open @14:35:41.87 SS.seek 0ml @14:35:50.86 Exhaust.select :close @14:35:50.96 SSV.dial CBV @14:35:50.98
CBV.dial SSV @14:35:51.75 Allowing 130s for 25.0ml of remaining 50.0ml @14:35:51.76 Intake.select :open @14:35:53.91 Intake @15.6psia, vacuum from 10.0 to 13.0psi @14:35:53.93 SS.reconfigure :inhaling 0.31ml/s @14:35:53.97 SS.seek 25ml @14:37:27.96 Stroke completed -- waiting for pressure to equalize ... @14:37:27.97 SS.reconfigure :exhale @14:37:28.01 SS.hold @14:38:01.77 SS.reconfigure :default @14:38:01.81 SS.hold @14:38:01.91 Intake.select :close @14:38:02.05 ShallowSampler::Clogged in MAIN -- Filtering too Slowly after 975.0ml @14:38:02.06 SSV.dial :exhaust @14:38:02.82 Exhaust.select :open @14:38:02.93 SS.reconfigure :exhale @14:38:02.97 SS.seek 0ml @14:38:11.82 Exhaust.select :close @14:38:11.93 Sampled 975.0ml @14:38:11.94 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @14:38:11.95 Intake.select :close @14:38:12.00 CSV.dial :waste @14:38:12.05 CS.seek 0ml @14:38:12.15 SSV.dial CBV,avoiding: :exhaust @14:38:12.17 CSV.dial :air @14:38:12.19
CBV.dial SSV @14:38:12.96 CS.seek 10ml @14:38:14.08
CTV.dial :air @14:38:14.86 delay 15 seconds @14:38:29.88 SSV.dial :spit,avoiding: :exhaust @14:38:31.20 CTV.dial CSV,avoiding: :intake @14:38:31.24
SS.seek 0ml @14:38:31.35 SSV.dial CBV,avoiding: :exhaust @14:38:37.65 CBV.dial between SSV and waste2 @14:38:38.02 CTV.dial CSV,avoiding: :air @14:38:38.05
CSV.dial CTV @14:38:38.49 CS.reconfigure :pressurize @14:38:38.53 CS.seek 0ml @14:38:57.28 BubblePoint: Intake @38.9psia, Outlet @14.9psia CBV.dial SSV @14:38:57.61 SS.reconfigure :default @14:38:57.65 SS.seek 10ml @14:39:03.23 delay 15 seconds @14:39:18.34 Evacuated: Intake @17.2psia, Outlet @9.5psia @14:39:18.35 SSV.dial :spit,avoiding: :exhaust @14:39:20.14 SS.reconfigure :exhale @14:39:20.18 SS.seek 0ml @14:39:25.47 SS.reconfigure :fast @14:39:25.51 SS.seek 5ml @14:39:28.52 SSV.dial CBV,avoiding: :exhaust @14:39:28.54
CBV.dial SSV @14:39:29.86 CTV.dial :air,avoiding: :intake @14:39:31.20 CSV.dial CTV @14:39:31.24
SS.seek 10ml @14:39:32.23 CSV.dial :air @14:39:32.69 CS.reconfigure :fast @14:39:32.73 CS.seek 0ml @14:39:33.84
SSV.dial :spit,avoiding: :exhaust @14:39:35.18 SS.reconfigure :exhale @14:39:35.22 SS.seek 0ml @14:39:40.22 SS.reconfigure :fast @14:39:40.26 SS.seek 5ml @14:39:40.33 CS.stop @14:39:43.08 SSV.dial CBV,avoiding: :exhaust @14:39:43.10
CBV.dial SSV @14:39:45.44 SSV.dial :spit,avoiding: :exhaust @14:39:46.77 SS.reconfigure :default @14:39:46.81 SS.seek 0ml @14:39:50.04 SS.stop @14:39:50.08 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) Intake.select :close @14:39:50.15 CSV.dial :waste @14:39:50.26 SSV.dial CBV,avoiding: :exhaust @14:39:50.28
CBV.dial SSV @14:39:50.93 CS.seek 0ml @14:39:50.99 CSV.dial :air @14:39:51.61
CTV.dial :air @14:39:51.62 delay 15 seconds @14:39:51.72 CS.seek 10ml @14:40:06.64
SSV.dial :spit,avoiding: :exhaust @14:40:07.96 CTV.dial CSV,avoiding: :intake @14:40:07.98
SS.reconfigure :exhale @14:40:08.07 SS.seek 0ml @14:40:08.46 SSV.dial CBV,avoiding: :exhaust @14:40:16.02 CBV.dial between SSV and waste2 @14:40:16.40 CTV.dial CSV,avoiding: :air @14:40:16.42
CSV.dial CTV @14:40:16.86 CS.reconfigure :pressurize @14:40:16.90 CS.seek 0ml @14:40:38.12 BubblePoint: Intake @38.0psia, Outlet @13.8psia @14:40:38.13 CBV.dial SSV @14:40:38.45 SS.reconfigure :default @14:40:38.49 SS.seek 10ml @14:40:44.07 delay 15 seconds @14:40:59.19 Evacuated: Intake @18.4psia, Outlet @11.1psia @14:40:59.20 SSV.dial :spit,avoiding: :exhaust @14:41:00.51 SS.reconfigure :exhale @14:41:00.56 SS.seek 0ml @14:41:06.02 SS.reconfigure :fast @14:41:06.06 SS.seek 5ml @14:41:08.83 SSV.dial CBV,avoiding: :exhaust @14:41:08.86
CBV.dial SSV @14:41:10.17 CTV.dial :air,avoiding: :intake @14:41:11.53 CSV.dial CTV @14:41:11.56
SS.seek 10ml @14:41:12.55 CSV.dial :air @14:41:13.00 CS.reconfigure :fast @14:41:13.04 CS.seek 0ml @14:41:14.06
SSV.dial :spit,avoiding: :exhaust @14:41:15.37 SS.reconfigure :exhale @14:41:15.42 SS.seek 0ml @14:41:18.24 CS.stop @14:41:20.42
SS.reconfigure :fast @14:41:20.46 SS.seek 5ml @14:41:23.47 SSV.dial CBV,avoiding: :exhaust @14:41:23.50
CBV.dial SSV @14:41:25.81 SSV.dial :spit,avoiding: :exhaust @14:41:27.12 SS.reconfigure :default @14:41:27.17 SS.seek 0ml @14:41:30.23 SS.stop @14:41:30.27 Lysing: Adding 2.0ml AqMeOH @14:41:30.28 CBV.dial between CSV and waste1,avoiding: :DBV @14:41:31.76 CTV.dial CSV @14:41:33.07 CSV.dial CSR @14:41:33.86 Pulling 1ml of CSR.AqMeOH @14:41:33.87 CSR.select :AqMeOH @14:41:33.98 CS.reconfigure :default @14:41:34.02 CS.seek 1ml @14:41:37.65 delay 5 seconds @14:41:42.67 CSR.select :air @14:41:42.82 CS.seek 6ml @14:41:56.46 delay 2 seconds @14:41:58.48 CSV.dial :waste2 @14:41:59.03 CS.reconfigure :fast @14:41:59.07 CS.seek 0ml @14:42:13.33 CSV.dial CSR @14:42:14.26 Pulling 2ml of CSR.AqMeOH @14:42:14.27 CSR.select :AqMeOH @14:42:14.38 CS.reconfigure :default @14:42:14.42 CS.seek 2ml @14:42:20.59 delay 5 seconds @14:42:25.60 CSR.select :air @14:42:25.76 CS.seek 5ml @14:42:34.40 delay 2 seconds @14:42:36.41 CSV.dial CTV @14:42:36.86 CS.seek 1ml @14:42:48.34 Before lysing Collection Puck Heater at 15.7C/38.8psia @14:42:48.36 Heating Puck.from 3, :sh1 in Collection Clamp to 70C for 10:00 @14:42:48.38 CH.seek 70C,8:00 @14:44:45.61 CH.hold 10:00,70C @14:44:45.78 Begun lysing Collection Puck Heater at 68.1C/47.1psia @14:54:45.81 Ended lysing Collection Puck Heater at 70C/46.3psia @14:54:45.83 CS.seek 0ml @14:54:49.17 CTV.dial between spare and air,avoiding: :intake @14:54:50.22 CSV.dial :air @14:54:50.66 CS.seek 5ml @14:55:04.26 CSV.dial CTV @14:55:04.70 delay 2 seconds @14:55:06.72 CSV.dial :air @14:55:07.17 delay 2 seconds @14:55:09.18 CSV.dial CTV @14:55:09.64 delay 2 seconds @14:55:11.65 CSV.dial :air @14:55:12.11 delay 2 seconds @14:55:14.13 CS.seek 0ml @14:55:27.90 Puck @75.6psia after final lysis pressurization @14:55:27.92 Recovering Lysate into Collection syringe @14:55:27.93 CSV.dial CBV @14:55:28.93 CBV.dial CSV @14:55:29.30 CS.seek 4.999ml @14:55:43.29 delay 5 seconds @14:55:48.31 CTV.dial :air @14:55:48.83 CS.seek 7ml @14:55:54.92 delay 5 seconds @14:55:59.93 CSV.dial :air2 @14:56:00.38 CS.seek 10ml @14:56:08.98 CSV.dial between air and CTV,avoiding: CBV @14:56:10.14 Lysing Complete @14:56:10.21 Storing sh1 puck in Collection Clamp to tube 2 @14:56:10.36 SC.reconfigure :tight @14:56:10.41 SC.seek 2 @14:56:13.93 SC.configure :limp @14:56:14.60 Elbow.reconfigure :tight @14:56:14.65 Elbow.seek 2 @14:56:16.45 Forearm.reconfigure :tight @14:56:16.49 Forearm.seek 2 + 450 counts @14:56:17.49 SE.reconfigure :pick @14:56:17.54 SE.seek :beamBreak @14:56:20.62 SE.configure :default @14:56:20.76 Elbow.reconfigure :limp @14:56:20.80 Forearm.reconfigure :loose @14:56:20.84 Forearm.seek 2 @14:56:21.10 Elbow.hold @14:56:21.16 Elbow.reconfigure :tight @14:56:21.25 SE.seek 2.17 Pucks below Top Plate @14:56:21.67 Forearm.force -20 @14:56:21.71 Forearm.seek 2 + 304 counts @14:56:21.98 Forearm.reconfigure :tight @14:56:22.03 Forearm.seek 2 + 450 counts @14:56:22.24 SE.seek 3.17 Pucks below Top Plate @14:56:22.80 Forearm.reconfigure :loose @14:56:22.84 Forearm.seek :retract @14:56:23.75 Elbow.seek CC @14:56:25.39 Elbow.reconfigure :loose @14:56:25.48 Forearm.seek CC @14:56:26.49 Hand.close @14:56:27.04 CC.seek :open @14:56:31.76 Forearm.seek :retract @14:56:32.78 Elbow.reconfigure :tight @14:56:32.82 Elbow.seek 2 @14:56:34.59 Elbow.reconfigure :limp @14:56:34.63 Forearm.seek 2 @14:56:35.60 Elbow.hold @14:56:35.67 Elbow.reconfigure :tight @14:56:35.71 Hand.open @14:56:36.40 Forearm.force -20 @14:56:36.43 Forearm.seek 2 + 312 counts @14:56:36.72 Forearm.reconfigure :tight @14:56:36.77 Forearm.seek 2 + 450 counts @14:56:37.01 SE.seek :down @14:56:38.13
Forearm.reconfigure :loose @14:56:38.18 Forearm.seek :retract @14:56:39.50 SE.stop @14:56:39.54
Tranferring DA @14:56:39.55 Beginning samplePassC @14:56:39.56 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:56:39.74 Elbow.seek FlushPuck::Garage @14:56:41.58 Elbow.reconfigure :loose @14:56:41.68 Forearm.seek :garage @14:56:42.60 Hand.close @14:56:43.28 Forearm.seek :retract @14:56:44.20 Elbow.reconfigure :tight @14:56:44.24 Elbow.seek CC @14:56:47.22 Elbow.reconfigure :loose @14:56:47.31 Forearm.reconfigure :tight @14:56:47.36 Forearm.seek CC @14:56:48.59 CC.seek :closed @14:56:53.10 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.29 @14:56:53.17 Hand.open @14:56:53.85 Forearm.reconfigure :loose @14:56:53.89 Forearm.seek :retract @14:56:54.90 PSV.dial PSR @14:56:56.27 Pulling 1.5ml of PSR.flush @14:56:56.28 PSR.select :flush @14:56:56.39 PS.seek 1.5ml @14:57:12.95 delay 5 seconds @14:57:17.97 PSV.dial :waste1 @14:57:18.44 PS.seek 0ml @14:57:34.57 PSV.dial PSR @14:57:35.03 PSR.select :air @14:57:35.14 PS.seek 2.5ml @14:58:01.49 PRV.dial CBV,avoiding: :ab2 @14:58:01.51
CBV.dial PRV @14:58:03.18 PSV.dial :mixloop @14:58:04.22 CTV.dial :air,avoiding: :intake @14:58:04.23 PTV.dial :PRVmixing @14:58:05.56 PS.seek 0ml @14:58:31.88 PSV.dial :air @14:58:32.35 PS.seek 2.5ml @14:58:58.70 PSV.dial :mixloop @14:58:59.16 PS.seek 0ml @14:59:25.51 PSV.dial :air @14:59:25.97 PS.seek 2.5ml @14:59:52.38 PSV.dial :mixloop @14:59:52.84 PS.seek 0ml @15:00:19.14 Garaging FlushPuck in Collection Clamp @15:00:19.21 Elbow.reconfigure :tight @15:00:19.25 Elbow.seek CC @15:00:19.62 Elbow.reconfigure :loose @15:00:19.71 Forearm.seek CC @15:00:20.73 Hand.close @15:00:21.29 CC.seek :open @15:00:25.90 Forearm.seek :retract @15:00:26.87 Elbow.reconfigure :tight @15:00:26.91 Elbow.seek :garage @15:00:29.84 Elbow.reconfigure :loose @15:00:29.93 Forearm.reconfigure :tight @15:00:29.97 Forearm.seek :garage @15:00:30.98 Hand.open @15:00:31.60 Forearm.reconfigure :loose @15:00:31.64 Forearm.seek :retract @15:00:32.60 PSV.dial :mixloop @15:00:32.61 PTV.dial :PRVmixing @15:00:32.63 PRV.dial CBV @15:00:32.65
CBV.dial PRV @15:00:32.66 CTV.dial :air,avoiding: :intake @15:00:32.68 Loading lyfil puck from tube 3 into Collection Clamp @15:00:32.89 SC.reconfigure :tight @15:00:32.94 SC.seek 3 @15:00:36.49 SC.configure :limp @15:00:36.67 Elbow.reconfigure :tight @15:00:36.72 Elbow.seek 3 @15:00:38.64 Forearm.reconfigure :tight @15:00:38.68 Forearm.seek 3 + 450 counts @15:00:39.72 SE.reconfigure :pick @15:00:39.77 SE.seek :beamBreak @15:00:40.96 SE.configure :default @15:00:41.10 Elbow.seek 3 @15:00:41.26 Forearm.seek 3 + 300 counts @15:00:41.43 Forearm.reconfigure :loose @15:00:41.47 Hand.close @15:00:42.05 SE.seek :down @15:00:42.24
Forearm.seek :retract @15:00:42.92 SE.stop @15:00:43.17
Elbow.seek CC @15:00:44.81 Elbow.reconfigure :loose @15:00:44.90 Forearm.reconfigure :tight @15:00:44.94 Forearm.seek CC @15:00:46.14 CC.seek :closed @15:00:51.18 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.09 @15:00:51.19 Hand.open @15:00:51.85 Forearm.reconfigure :loose @15:00:51.90 Forearm.seek :retract @15:00:52.93 CTV.dial CSV,avoiding: :intake @15:00:54.29 CSV.dial CTV @15:00:54.61 PS.seek 2.5ml @15:01:20.94 CS.seek 4.5ml @15:01:35.79 delay 8 seconds @15:01:43.81 PSV.dial :air @15:01:44.40 delay 2 seconds @15:01:46.41 PSV.dial :waste2 @15:01:47.17 PS.seek 0ml @15:02:13.57 CBV.dial between PRV and waste1 @15:02:13.90 CSV.dial :air @15:02:14.37 CS.seek 10ml @15:02:29.38 CSV.dial CTV @15:02:29.82 CS.seek 0ml @15:02:56.19 CBV.dial :waste1 @15:02:56.49 delay 5 seconds @15:03:01.51 CTV.dial :air,avoiding: :intake @15:03:02.91 Storing lyfil puck in Collection Clamp to tube 2 @15:03:03.06 SC.reconfigure :tight @15:03:03.10 SC.seek 2 @15:03:06.72 SC.configure :limp @15:03:06.91 Elbow.reconfigure :tight @15:03:06.96 Elbow.seek 2 @15:03:08.73 Forearm.reconfigure :tight @15:03:08.77 Forearm.seek 2 + 450 counts @15:03:09.77 SE.reconfigure :pick @15:03:09.82 SE.seek :beamBreak @15:03:12.78 SE.configure :default @15:03:12.92 Elbow.reconfigure :limp @15:03:12.96 Forearm.reconfigure :loose @15:03:13.00 Forearm.seek 2 @15:03:13.16 Elbow.hold @15:03:13.22 Elbow.reconfigure :tight @15:03:13.31 SE.seek 3.16 Pucks below Top Plate @15:03:13.73 Forearm.force -20 @15:03:13.76 Forearm.seek 2 + 319 counts @15:03:14.15 Forearm.reconfigure :tight @15:03:14.20 Forearm.seek 2 + 450 counts @15:03:14.42 SE.seek 4.16 Pucks below Top Plate @15:03:14.92 Forearm.reconfigure :loose @15:03:14.96 Forearm.seek :retract @15:03:15.87 Elbow.seek CC @15:03:17.57 Elbow.reconfigure :loose @15:03:17.67 Forearm.seek CC @15:03:18.63 Hand.close @15:03:19.29 CC.seek :open @15:03:24.03 Forearm.seek :retract @15:03:25.05 Elbow.reconfigure :tight @15:03:25.10 Elbow.seek 2 @15:03:26.89 Elbow.reconfigure :limp @15:03:26.94 Forearm.seek 2 @15:03:27.91 Elbow.hold @15:03:27.97 Elbow.reconfigure :tight @15:03:28.01 Hand.open @15:03:28.77 Forearm.force -20 @15:03:28.80 Forearm.seek 2 + 305 counts @15:03:29.10 Forearm.reconfigure :tight @15:03:29.14 Forearm.seek 2 + 450 counts @15:03:29.38 SE.seek :down @15:03:30.50
Forearm.reconfigure :loose @15:03:30.54 Forearm.seek :retract @15:03:31.82 SE.stop @15:03:31.86
PSV.dial :air @15:03:33.08 PS.seek 2.5ml @15:03:59.47 delay 1 second @15:04:00.49 PSV.dial :mixloop @15:04:00.93 delay 1 second @15:04:01.94 PSV.dial :air @15:04:02.40 delay 1 second @15:04:03.41 PSV.dial :mixloop @15:04:03.88 delay 1 second @15:04:04.90 PSV.dial :air @15:04:05.37 delay 1 second @15:04:06.39 PS.seek 0ml @15:04:32.79 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @15:04:32.96 Elbow.seek FlushPuck::Garage @15:04:34.76 Elbow.reconfigure :loose @15:04:34.85 Forearm.seek :garage @15:04:35.86 Hand.close @15:04:36.52 Forearm.seek :retract @15:04:37.51 Elbow.reconfigure :tight @15:04:37.55 Elbow.seek PC @15:04:38.78 Elbow.reconfigure :loose @15:04:38.87 Forearm.reconfigure :tight @15:04:38.91 Forearm.seek PC @15:04:40.18 PC.seek :closed @15:04:44.83 Processing Clamp onSpring/offSpring for 16/26 samples, on/off torque 1.55 @15:04:44.90 Hand.open @15:04:45.59 Forearm.reconfigure :loose @15:04:45.63 Forearm.seek :retract @15:04:46.58 PRV.dial :air @15:04:47.02 PSV.dial :mixloop @15:04:47.53 PS.seek 0.3ml @15:04:51.57 PSV.dial PSR @15:04:52.65 Pulling 0.4ml of PSR.air @15:04:52.66 PSR.select :air @15:04:52.68 PS.seek 0.7ml @15:04:57.54 delay 3 seconds @15:05:00.55 PSR.select :air @15:05:00.57 PS.seek 2.5ml @15:05:19.75 delay 3 seconds @15:05:22.77 PSV.dial :mixloop @15:05:23.80 PTV.dial :mixing @15:05:24.55 PBV.dial :waste2 @15:05:25.50 PS.seek 0ml @15:05:51.82 Garaging FlushPuck in Processing Clamp @15:05:51.89 Elbow.reconfigure :tight @15:05:51.93 Elbow.seek PC @15:05:52.04 Elbow.reconfigure :loose @15:05:52.13 Forearm.seek PC @15:05:53.09 Hand.close @15:05:53.66 PC.seek :open @15:05:58.36 Forearm.seek :retract @15:05:59.35 Elbow.reconfigure :tight @15:05:59.39 Elbow.seek :garage @15:06:00.73 Elbow.reconfigure :loose @15:06:00.82 Forearm.reconfigure :tight @15:06:00.86 Forearm.seek :garage @15:06:01.89 Hand.open @15:06:02.59 Forearm.reconfigure :loose @15:06:02.63 Forearm.seek :retract @15:06:03.62 Loading da puck from tube 3 into Processing Clamp @15:06:03.83 SC.reconfigure :tight @15:06:03.87 SC.seek 3 @15:06:07.47 SC.configure :limp @15:06:07.65 Elbow.reconfigure :tight @15:06:07.69 Elbow.seek 3 @15:06:09.58 Forearm.reconfigure :tight @15:06:09.62 Forearm.seek 3 + 450 counts @15:06:10.64 SE.reconfigure :pick @15:06:10.69 SE.seek :beamBreak @15:06:11.98 SE.configure :default @15:06:12.12 Elbow.seek 3 @15:06:12.77 Forearm.seek 3 + 300 counts @15:06:12.94 Forearm.reconfigure :loose @15:06:12.98 Hand.close @15:06:13.55 SE.seek :down @15:06:13.74
Forearm.seek :retract @15:06:14.50 SE.stop @15:06:14.69
Elbow.seek PC @15:06:17.23 Elbow.reconfigure :loose @15:06:17.32 Forearm.reconfigure :tight @15:06:17.36 Forearm.seek PC @15:06:18.54 PC.seek :closed @15:06:23.17 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.59 @15:06:23.18 Hand.open @15:06:23.84 Forearm.reconfigure :loose @15:06:23.88 Forearm.seek :retract @15:06:24.83 Heating Puck.from 3, :da in Processing Clamp to 28C for 2:00:00 @15:06:24.85 PH.seek 28C,5:00 @15:07:01.53 PH.hold 2:00:00,28C @15:07:01.58 delay 1:30 @15:08:31.60 PSR.select :air @15:08:31.61 PSV.dial PSR @15:08:32.71 Pulling 0.5ml of PSR.wash @15:08:32.72 PSR.select :wash @15:08:32.83 PS.seek 0.5ml @15:08:38.85 delay 5 seconds @15:08:43.86 PSR.select :air @15:08:43.98 PS.seek 2ml @15:09:00.06 PTV.dial :puck @15:09:00.78 PSV.dial PTV @15:09:01.55 PBV.dial :waste1 @15:09:02.93 PS.seek 1.5ml @15:09:08.97 delay 3 seconds @15:09:11.98 PBV.dial between PSV and waste1 @15:09:12.34 PS.seek 0ml @15:09:28.53 delay 5:00 @15:14:28.55 recoverPSRreagent08 @15:14:28.56 PTV.dial :puck @15:14:28.57 PBV.dial :waste1 @15:14:28.93 delay 30 seconds @15:14:58.96 PBV.dial between PSV and waste1 @15:14:59.31 PSR.select :air @15:14:59.32 PSV.dial PSR @15:15:00.09 PS.reconfigure :fast @15:15:00.13 PS.seek 2.5ml @15:15:23.80 PSV.dial PTV @15:15:24.56 delay 5 seconds @15:15:29.57 PSV.dial PSR @15:15:30.34 delay 5 seconds @15:15:35.36 PSV.dial PTV @15:15:36.12 delay 5 seconds @15:15:41.14 PSV.dial :air @15:15:42.02 PS.seek 0ml @15:16:05.15 PTV.dial :PRVmixing,via: PRV @15:16:06.46 PSV.dial :mixloop @15:16:06.90 PRV.dial :ab1 @15:16:07.65 PS.reconfigure :slow @15:16:07.70 PS.seek 0.15ml @15:16:11.27 delay 2 seconds @15:16:13.29 CBV.dial PRV @15:16:13.31
PRV.dial CBV @15:16:13.87 PS.seek 0.35ml @15:16:18.46 delay 2 seconds @15:16:20.48 CBV.dial between PRV and PSV @15:16:20.79 PRV.dial :air @15:16:21.23 PS.seek 0.9ml @15:16:33.02 PRV.dial :waste2 @15:16:34.24 PS.seek 0.5ml @15:16:42.90 addPMLReagent08 @15:16:42.91 PSV.dial :air @15:16:43.37 PS.reconfigure :default @15:16:43.41 PS.seek 2ml @15:16:59.65 PSV.dial :PML @15:17:00.10 PTV.dial :mixing @15:17:00.85 PBV.dial :waste1 @15:17:01.27 PS.seek 1.85ml @15:17:03.62 PBV.dial between PSV and waste1 @15:17:04.00 PS.seek 0ml @15:17:23.74 delay 2 seconds @15:17:25.76 Beginning samplePassC @15:17:25.77 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @15:17:25.93 Elbow.reconfigure :tight @15:17:25.98 Elbow.seek FlushPuck::Garage @15:17:27.27 Elbow.reconfigure :loose @15:17:27.36 Forearm.seek :garage @15:17:28.38 Hand.close @15:17:29.14 Forearm.seek :retract @15:17:30.15 Elbow.reconfigure :tight @15:17:30.20 Elbow.seek CC @15:17:33.03 Elbow.reconfigure :loose @15:17:33.12 Forearm.reconfigure :tight @15:17:33.16 Forearm.seek CC @15:17:34.35 CC.seek :closed @15:17:38.85 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.37 @15:17:38.92 Hand.open @15:17:39.58 Forearm.reconfigure :loose @15:17:39.62 Forearm.seek :retract @15:17:40.55 PSV.dial PSR @15:17:41.78 Pulling 1.5ml of PSR.flush @15:17:41.79 PSR.select :flush @15:17:41.90 PS.seek 1.5ml @15:17:58.09 delay 5 seconds @15:18:03.10 PSV.dial :waste1 @15:18:03.58 PS.seek 0ml @15:18:19.92 PSV.dial PSR @15:18:20.38 PSR.select :air @15:18:20.49 PS.seek 2.5ml @15:18:46.87 PRV.dial CBV,avoiding: :ab2 @15:18:46.89
CBV.dial PRV @15:18:48.50 PSV.dial :mixloop @15:18:49.66 CTV.dial :air,avoiding: :intake @15:18:49.67 PTV.dial :PRVmixing @15:18:50.43 PS.seek 0ml @15:19:16.77 PSV.dial :air @15:19:17.23 PS.seek 2.5ml @15:19:43.58 PSV.dial :mixloop @15:19:44.04 PS.seek 0ml @15:20:10.36 PSV.dial :air @15:20:10.82 PS.seek 2.5ml @15:20:37.14 PSV.dial :mixloop @15:20:37.60 PS.seek 0ml @15:21:03.96 Garaging FlushPuck in Collection Clamp @15:21:04.02 Elbow.reconfigure :tight @15:21:04.07 Elbow.seek CC @15:21:04.34 Elbow.reconfigure :loose @15:21:04.43 Forearm.seek CC @15:21:05.46 Hand.close @15:21:06.03 CC.seek :open @15:21:10.66 Forearm.seek :retract @15:21:11.63 Elbow.reconfigure :tight @15:21:11.68 Elbow.seek :garage @15:21:14.62 Elbow.reconfigure :loose @15:21:14.71 Forearm.reconfigure :tight @15:21:14.76 Forearm.seek :garage @15:21:15.82 Hand.open @15:21:16.92 Forearm.reconfigure :loose @15:21:16.96 Forearm.seek :retract @15:21:17.92 delayUntil "15:25:25.75" @15:21:17.94 Whole Cell Archive sampling 1000ml @15:21:17.96 Loading wcr puck from tube 3 into Collection Clamp @15:21:18.18 SC.reconfigure :tight @15:21:18.23 SC.seek 3 @15:21:18.29 SC.configure :limp @15:21:18.49 Elbow.reconfigure :tight @15:21:18.54 Elbow.seek 3 @15:21:20.50 Forearm.reconfigure :tight @15:21:20.55 Forearm.seek 3 + 450 counts @15:21:21.60 SE.reconfigure :pick @15:21:21.66 SE.seek :beamBreak @15:21:23.08 SE.configure :default @15:21:23.23 Elbow.seek 3 @15:21:23.41 Forearm.seek 3 + 300 counts @15:21:23.63 Forearm.reconfigure :loose @15:21:23.68 Hand.close @15:21:24.29 SE.seek :down @15:21:24.47 Forearm.seek :retract @15:21:25.31 SE.stop @15:21:25.46 Elbow.seek CC @15:21:27.10 Elbow.reconfigure :loose @15:21:27.20 Forearm.reconfigure :tight @15:21:27.24 Forearm.seek CC @15:21:28.46 CC.seek :closed @15:21:32.98 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.18 @15:21:33.00 Hand.open @15:21:33.71 Forearm.reconfigure :loose @15:21:33.75 Forearm.seek :retract @15:21:34.78 collectSample 1000ml w/bubble point of 35psi @15:21:34.79 Priming 7ml @15:21:34.80 Intake.select :close @15:21:34.81 Exhaust.select :close @15:21:34.82 SS.hold @15:21:35.01 Calibrating pressure sensors @15:21:35.02 CTV.dial :air @15:21:35.03 SSV.dial :spit,avoiding: :exhaust @15:21:35.05 SS.reconfigure :fast @15:21:35.09 SS.seek 5ml @15:21:37.78 SSV.dial CBV @15:21:37.80 CBV.dial SSV @15:21:39.15 delay 3.8 seconds @15:21:43.08 SSV.dial :spit @15:21:44.41 SS.seek 0ml @15:21:47.18 SSV.dial CBV @15:21:48.49 CTV.dial :intake @15:21:49.25 SSV.dial CBV @15:21:49.28 CBV.dial SSV @15:21:49.29 Allowing 40s for 7.0ml of remaining 7.0ml @15:21:49.30 Intake.select :open @15:21:51.46 Intake @15.2psia, vacuum from 9.7 to 12.7psi @15:21:51.48 SS.reconfigure :inhaling 2.20ml/s @15:21:51.52 SS.seek 7ml @15:21:58.16 Stroke completed -- waiting for pressure to equalize ... @15:21:58.17 SS.reconfigure :exhale @15:21:58.22 SS.hold @15:22:07.89 Intake.select :close @15:22:08.04 SSV.dial :exhaust @15:22:08.81 Exhaust.select :open @15:22:08.92 SS.seek 0ml @15:22:12.93 Exhaust.select :close @15:22:13.04 Sampling 1000.0ml @15:22:13.05 Intake.select :close @15:22:13.06 Exhaust.select :close @15:22:13.07 SS.reconfigure :default @15:22:13.11 SS.hold @15:22:13.30 CTV.dial :intake @15:22:13.32 SSV.dial CBV @15:22:13.35 CBV.dial SSV @15:22:14.13 Allowing 130s for 25.0ml of remaining 1000.0ml @15:22:14.14 Intake.select :open @15:22:16.29 Intake @16.0psia, vacuum from 10.0 to 13.0psi @15:22:16.31 SS.reconfigure :inhaling 2.20ml/s @15:22:16.35 SS.seek 25ml @15:22:22.72 Vacuum too high -- waiting up to 40 seconds @15:22:22.73 SS.reconfigure :exhale @15:22:22.77 SS.hold @15:22:26.63 Intake @15.6psia, vacuum from 10.0 to 13.0psi @15:22:26.65 SS.reconfigure :inhaling 1.98ml/s @15:22:26.69 SS.seek 25ml @15:22:33.91 Vacuum too high -- waiting up to 40 seconds @15:22:33.92 SS.reconfigure :exhale @15:22:33.96 SS.hold @15:22:40.68 Intake @16.5psia, vacuum from 10.0 to 13.0psi @15:22:40.70 SS.reconfigure :inhaling 1.79ml/s @15:22:40.74 SS.seek 25ml @15:22:44.49 Stroke completed -- waiting for pressure to equalize ... @15:22:44.50 SS.reconfigure :exhale @15:22:44.54 SS.hold @15:22:49.40 Intake.select :close @15:22:49.55 SSV.dial :exhaust @15:22:50.30 Exhaust.select :open @15:22:50.41 SS.seek 0ml @15:22:59.21 Exhaust.select :close @15:22:59.32 SSV.dial CBV @15:22:59.34 CBV.dial SSV @15:23:00.12 Allowing 130s for 25.0ml of remaining 975.0ml @15:23:00.13 Intake.select :open @15:23:02.28 Intake @16.4psia, vacuum from 10.0 to 13.0psi @15:23:02.30 SS.reconfigure :inhaling 1.79ml/s @15:23:02.34 SS.seek 25ml @15:23:09.91 Vacuum too high -- waiting up to 40 seconds @15:23:09.92 SS.reconfigure :exhale @15:23:09.96 SS.hold @15:23:13.14 Intake @15.6psia, vacuum from 10.0 to 13.0psi @15:23:13.16 SS.reconfigure :inhaling 1.61ml/s @15:23:13.20 SS.seek 25ml @15:23:24.47 Stroke completed -- waiting for pressure to equalize ... @15:23:24.48 SS.reconfigure :exhale @15:23:24.53 SS.hold @15:23:35.82 Intake.select :close @15:23:35.96 SSV.dial :exhaust @15:23:36.72 Exhaust.select :open @15:23:36.84 SS.seek 0ml @15:23:45.63 Exhaust.select :close @15:23:45.74 SSV.dial CBV @15:23:45.77 CBV.dial SSV @15:23:46.54 Allowing 130s for 25.0ml of remaining 950.0ml @15:23:46.55 Intake.select :open @15:23:48.71 Intake @16.3psia, vacuum from 10.0 to 13.0psi @15:23:48.73 SS.reconfigure :inhaling 1.61ml/s @15:23:48.77 SS.seek 25ml @15:23:57.28 Vacuum too high -- waiting up to 40 seconds @15:23:57.29 SS.reconfigure :exhale @15:23:57.33 SS.hold @15:23:59.52 Intake @14.6psia, vacuum from 9.1 to 12.1psi @15:23:59.54 SS.reconfigure :inhaling 1.45ml/s @15:23:59.58 SS.seek 25ml @15:24:10.82 Stroke completed -- waiting for pressure to equalize ... @15:24:10.83 SS.reconfigure :exhale @15:24:10.87 SS.hold @15:24:19.43 Intake.select :close @15:24:19.57 SSV.dial :exhaust @15:24:20.33 Exhaust.select :open @15:24:20.44 SS.seek 0ml @15:24:29.24 Exhaust.select :close @15:24:29.35 SSV.dial CBV @15:24:29.38 CBV.dial SSV @15:24:30.13 Allowing 130s for 25.0ml of remaining 925.0ml @15:24:30.14 Intake.select :open @15:24:32.30 Intake @16.3psia, vacuum from 10.0 to 13.0psi @15:24:32.32 SS.reconfigure :inhaling 1.45ml/s @15:24:32.36 SS.seek 25ml @15:24:43.06 Vacuum too high -- waiting up to 40 seconds @15:24:43.07 SS.reconfigure :exhale @15:24:43.11 SS.hold @15:24:45.75 Intake @14.7psia, vacuum from 9.2 to 12.2psi @15:24:45.77 SS.reconfigure :inhaling 1.30ml/s @15:24:45.81 SS.seek 25ml @15:24:56.91 Stroke completed -- waiting for pressure to equalize ... @15:24:56.92 SS.reconfigure :exhale @15:24:56.96 SS.hold @15:25:05.54 Intake.select :close @15:25:05.68 SSV.dial :exhaust @15:25:06.44 Exhaust.select :open @15:25:06.55 SS.seek 0ml @15:25:15.52 Exhaust.select :close @15:25:16.13 SSV.dial CBV @15:25:16.16 CBV.dial SSV @15:25:16.93 Allowing 130s for 25.0ml of remaining 900.0ml @15:25:16.94 Intake.select :open @15:25:19.10 Intake @16.1psia, vacuum from 10.0 to 13.0psi @15:25:19.11 SS.reconfigure :inhaling 1.30ml/s @15:25:19.16 SS.seek 25ml @15:25:25.76 recoverPRVreagent08 @15:25:25.77 PTV.dial :mixing @15:25:26.53 PBV.dial :waste1 @15:25:26.90 delay 30 seconds @15:25:31.50 Vacuum too high -- waiting up to 40 seconds @15:25:31.51 SS.reconfigure :exhale @15:25:31.55 SS.hold @15:25:35.15 Intake @15.1psia, vacuum from 9.6 to 12.6psi @15:25:35.17 SS.reconfigure :inhaling 1.17ml/s @15:25:35.21 SS.seek 25ml @15:25:45.89 Stroke completed -- waiting for pressure to equalize ... @15:25:45.90 SS.reconfigure :exhale @15:25:45.95 SS.hold @15:25:53.32 Intake.select :close @15:25:53.46 SSV.dial :exhaust @15:25:54.22 Exhaust.select :open @15:25:54.33 SS.seek 0ml @15:25:56.92 PBV.dial between PSV and waste1 @15:25:57.30 PSV.dial :air @15:25:57.77 PS.reconfigure :fast @15:25:57.81 PS.seek 2.5ml @15:26:03.14 Exhaust.select :close @15:26:03.25 SSV.dial CBV @15:26:03.27 CBV.dial SSV @15:26:04.05 Allowing 130s for 25.0ml of remaining 875.0ml @15:26:04.06 Intake.select :open @15:26:06.22 Intake @16.2psia, vacuum from 10.0 to 13.0psi @15:26:06.24 SS.reconfigure :inhaling 1.17ml/s @15:26:06.28 SS.seek 25ml @15:26:21.03 Vacuum too high -- waiting up to 40 seconds @15:26:21.04 SS.reconfigure :exhale @15:26:21.08 SS.hold @15:26:21.74 PSV.dial :mixloop @15:26:22.20 delay 2 seconds @15:26:24.11 Intake @14.8psia, vacuum from 9.3 to 12.3psi @15:26:24.13 SS.reconfigure :inhaling 1.06ml/s @15:26:24.17 SS.seek 25ml @15:26:24.22 PSV.dial :air @15:26:24.68 delay 2 seconds @15:26:26.70 PSV.dial :mixloop @15:26:27.17 delay 2 seconds @15:26:29.19 PSV.dial :air @15:26:29.80 PS.seek 0ml @15:26:34.99 Stroke completed -- waiting for pressure to equalize ... @15:26:35.00 SS.reconfigure :exhale @15:26:35.04 SS.hold @15:26:42.27 Intake.select :close @15:26:42.42 SSV.dial :exhaust @15:26:43.17 Exhaust.select :open @15:26:43.29 SS.seek 0ml @15:26:52.31 Exhaust.select :close @15:26:52.42 SSV.dial CBV @15:26:52.44 CBV.dial SSV @15:26:53.14 washCycleDA #1 of 2 @15:26:53.15 PSR.select :air @15:26:53.16 PSV.dial PSR @15:26:53.23 Allowing 130s for 25.0ml of remaining 850.0ml @15:26:53.24 Intake.select :open @15:26:54.53 Pulling 0.5ml of PSR.wash @15:26:54.54 PSR.select :wash @15:26:54.66 PS.reconfigure :default @15:26:54.71 PS.seek 0.5ml @15:26:55.39 Intake @16.0psia, vacuum from 10.0 to 13.0psi @15:26:55.41 SS.reconfigure :inhaling 1.06ml/s @15:26:55.46 SS.seek 25ml @15:27:00.67 delay 5 seconds @15:27:05.69 PSR.select :air @15:27:05.80 PS.seek 2ml @15:27:15.84 Vacuum too high -- waiting up to 40 seconds @15:27:15.85 SS.reconfigure :exhale @15:27:15.89 SS.hold @15:27:20.06 Intake @15.1psia, vacuum from 9.6 to 12.6psi @15:27:20.08 SS.reconfigure :inhaling 0.95ml/s @15:27:20.12 SS.seek 25ml @15:27:21.95 PTV.dial :puck @15:27:22.70 PSV.dial PTV @15:27:23.46 PBV.dial :waste1 @15:27:23.91 PS.seek 1.5ml @15:27:26.23 Stroke completed -- waiting for pressure to equalize ... @15:27:26.25 SS.reconfigure :exhale @15:27:26.29 SS.hold @15:27:29.91 delay 3 seconds @15:27:32.70 Intake.select :close @15:27:32.84 SSV.dial :exhaust @15:27:32.93 PBV.dial between PSV and waste1 @15:27:33.31 PS.seek 0ml @15:27:33.60 Exhaust.select :open @15:27:33.71 SS.seek 0ml @15:27:42.70 Exhaust.select :close @15:27:42.81 SSV.dial CBV @15:27:42.83 CBV.dial SSV @15:27:43.60 Allowing 130s for 25.0ml of remaining 825.0ml @15:27:43.62 Intake.select :open @15:27:45.77 Intake @16.0psia, vacuum from 10.0 to 13.0psi @15:27:45.79 SS.reconfigure :inhaling 0.95ml/s @15:27:45.84 SS.seek 25ml @15:27:49.64 delay 2:00 @15:28:14.15 Stroke completed -- waiting for pressure to equalize ... @15:28:14.17 SS.reconfigure :exhale @15:28:14.21 SS.hold @15:28:23.97 Intake.select :close @15:28:24.11 SSV.dial :exhaust @15:28:24.88 Exhaust.select :open @15:28:24.99 SS.seek 0ml @15:28:34.00 Exhaust.select :close @15:28:34.11 SSV.dial CBV @15:28:34.14 CBV.dial SSV @15:28:34.89 Allowing 130s for 25.0ml of remaining 800.0ml @15:28:34.91 Intake.select :open @15:28:37.06 Intake @16.0psia, vacuum from 10.0 to 13.0psi @15:28:37.08 SS.reconfigure :inhaling 0.95ml/s @15:28:37.12 SS.seek 25ml @15:29:01.37 Vacuum too high -- waiting up to 40 seconds @15:29:01.38 SS.reconfigure :exhale @15:29:01.42 SS.hold @15:29:05.47 Intake @14.7psia, vacuum from 9.2 to 12.2psi @15:29:05.49 SS.reconfigure :inhaling 0.87ml/s @15:29:05.53 SS.seek 25ml @15:29:10.40 Stroke completed -- waiting for pressure to equalize ... @15:29:10.42 SS.reconfigure :exhale @15:29:10.46 SS.hold @15:29:16.88 Intake.select :close @15:29:17.03 SSV.dial :exhaust @15:29:17.80 Exhaust.select :open @15:29:17.91 SS.seek 0ml @15:29:26.67 Exhaust.select :close @15:29:26.78 SSV.dial CBV @15:29:26.80 CBV.dial SSV @15:29:27.58 Allowing 130s for 25.0ml of remaining 775.0ml @15:29:27.59 Intake.select :open @15:29:29.75 Intake @16.0psia, vacuum from 10.0 to 13.0psi @15:29:29.77 SS.reconfigure :inhaling 0.87ml/s @15:29:29.81 SS.seek 25ml @15:29:49.67 recoverPSRreagent08 @15:29:49.68 PTV.dial :puck @15:29:49.69 PBV.dial :waste1 @15:29:50.07 delay 30 seconds @15:30:01.26 Stroke completed -- waiting for pressure to equalize ... @15:30:01.27 SS.reconfigure :exhale @15:30:01.32 SS.hold @15:30:12.34 Intake.select :close @15:30:12.48 SSV.dial :exhaust @15:30:13.25 Exhaust.select :open @15:30:13.36 SS.seek 0ml @15:30:20.09 PBV.dial between PSV and waste1 @15:30:20.46 PSR.select :air @15:30:20.48 PSV.dial PSR @15:30:21.25 PS.reconfigure :fast @15:30:21.29 PS.seek 2.5ml @15:30:22.17 Exhaust.select :close @15:30:22.28 SSV.dial CBV @15:30:22.30 CBV.dial SSV @15:30:23.08 Allowing 130s for 25.0ml of remaining 750.0ml @15:30:23.09 Intake.select :open @15:30:25.25 Intake @15.9psia, vacuum from 10.0 to 13.0psi @15:30:25.27 SS.reconfigure :inhaling 0.87ml/s @15:30:25.31 SS.seek 25ml @15:30:45.19 PSV.dial PTV @15:30:45.96 delay 5 seconds @15:30:50.98 PSV.dial PSR @15:30:51.73 delay 5 seconds @15:30:55.53 Vacuum too high -- waiting up to 40 seconds @15:30:55.54 SS.reconfigure :exhale @15:30:55.58 SS.hold @15:30:56.80 PSV.dial PTV @15:30:57.60 delay 5 seconds @15:31:01.23 Intake @15.0psia, vacuum from 9.5 to 12.5psi @15:31:01.25 SS.reconfigure :inhaling 0.78ml/s @15:31:01.30 SS.seek 25ml @15:31:02.62 PSV.dial :air @15:31:03.12 Stroke completed -- waiting for pressure to equalize ... @15:31:03.13 SS.reconfigure :exhale @15:31:03.18 SS.hold @15:31:03.90 PS.seek 0ml @15:31:08.40 Intake.select :close @15:31:08.54 SSV.dial :exhaust @15:31:09.29 Exhaust.select :open @15:31:09.40 SS.seek 0ml @15:31:18.40 Exhaust.select :close @15:31:18.51 SSV.dial CBV @15:31:18.53 CBV.dial SSV @15:31:19.30 Allowing 130s for 25.0ml of remaining 725.0ml @15:31:19.31 Intake.select :open @15:31:21.46 Intake @15.9psia, vacuum from 10.0 to 13.0psi @15:31:21.48 SS.reconfigure :inhaling 0.78ml/s @15:31:21.52 SS.seek 25ml @15:31:27.32 washCycleDA #2 of 2 @15:31:27.33 PSR.select :air @15:31:27.35 PSV.dial PSR @15:31:28.72 Pulling 0.5ml of PSR.wash @15:31:28.73 PSR.select :wash @15:31:28.84 PS.reconfigure :default @15:31:28.88 PS.seek 0.5ml @15:31:34.85 delay 5 seconds @15:31:39.87 PSR.select :air @15:31:39.98 PS.seek 2ml @15:31:55.66 Stroke completed -- waiting for pressure to equalize ... @15:31:55.67 SS.reconfigure :exhale @15:31:55.71 SS.hold @15:31:56.20 PTV.dial :puck @15:31:56.24 PSV.dial PTV @15:31:56.99 PBV.dial :waste1 @15:31:57.41 PS.seek 1.5ml @15:32:03.32 delay 3 seconds @15:32:06.33 PBV.dial between PSV and waste1 @15:32:06.74 PS.seek 0ml @15:32:06.91 Intake.select :close @15:32:07.05 SSV.dial :exhaust @15:32:07.81 Exhaust.select :open @15:32:07.92 SS.seek 0ml @15:32:16.74 Exhaust.select :close @15:32:16.85 SSV.dial CBV @15:32:16.88 CBV.dial SSV @15:32:17.64 Allowing 130s for 25.0ml of remaining 700.0ml @15:32:17.65 Intake.select :open @15:32:19.81 Intake @15.8psia, vacuum from 10.0 to 13.0psi @15:32:19.83 SS.reconfigure :inhaling 0.78ml/s @15:32:19.87 SS.seek 25ml @15:32:22.88 delay 2:00 @15:32:54.59 Stroke completed -- waiting for pressure to equalize ... @15:32:54.60 SS.reconfigure :exhale @15:32:54.64 SS.hold @15:33:05.67 Intake.select :close @15:33:05.81 SSV.dial :exhaust @15:33:06.57 Exhaust.select :open @15:33:06.68 SS.seek 0ml @15:33:15.56 Exhaust.select :close @15:33:15.66 SSV.dial CBV @15:33:15.69 CBV.dial SSV @15:33:16.50 Allowing 130s for 25.0ml of remaining 675.0ml @15:33:16.51 Intake.select :open @15:33:18.67 Intake @16.0psia, vacuum from 10.0 to 13.0psi @15:33:18.69 SS.reconfigure :inhaling 0.78ml/s @15:33:19.23 SS.seek 25ml @15:33:52.64 Vacuum too high -- waiting up to 40 seconds @15:33:52.65 SS.reconfigure :exhale @15:33:52.70 SS.hold @15:33:57.57 Intake @14.8psia, vacuum from 9.3 to 12.3psi @15:33:57.59 SS.reconfigure :inhaling 0.70ml/s @15:33:57.64 SS.seek 25ml @15:33:59.26 Stroke completed -- waiting for pressure to equalize ... @15:33:59.27 SS.reconfigure :exhale @15:33:59.31 SS.hold @15:34:05.74 Intake.select :close @15:34:05.88 SSV.dial :exhaust @15:34:06.65 Exhaust.select :open @15:34:06.76 SS.seek 0ml @15:34:15.55 Exhaust.select :close @15:34:15.66 SSV.dial CBV @15:34:15.69 CBV.dial SSV @15:34:16.45 Allowing 130s for 25.0ml of remaining 650.0ml @15:34:16.46 Intake.select :open @15:34:18.61 Intake @15.8psia, vacuum from 10.0 to 13.0psi @15:34:18.63 SS.reconfigure :inhaling 0.70ml/s @15:34:18.68 SS.seek 25ml @15:34:22.90 recoverPSRreagent08 @15:34:22.91 PTV.dial :puck @15:34:22.92 PBV.dial :waste1 @15:34:23.29 delay 30 seconds @15:34:53.31 PBV.dial between PSV and waste1 @15:34:53.67 PSR.select :air @15:34:53.69 PSV.dial PSR @15:34:54.46 PS.reconfigure :fast @15:34:54.51 PS.seek 2.5ml @15:34:56.68 Stroke completed -- waiting for pressure to equalize ... @15:34:56.69 SS.reconfigure :exhale @15:34:56.73 SS.hold @15:35:07.78 Intake.select :close @15:35:07.92 SSV.dial :exhaust @15:35:08.68 Exhaust.select :open @15:35:08.79 SS.seek 0ml @15:35:17.58 Exhaust.select :close @15:35:17.69 SSV.dial CBV @15:35:17.71 CBV.dial SSV @15:35:18.13 PSV.dial PTV @15:35:18.49 Allowing 130s for 25.0ml of remaining 625.0ml @15:35:18.51 Intake.select :open @15:35:18.89 delay 5 seconds @15:35:20.66 Intake @15.8psia, vacuum from 10.0 to 13.0psi @15:35:20.68 SS.reconfigure :inhaling 0.70ml/s @15:35:20.72 SS.seek 25ml @15:35:23.91 PSV.dial PSR @15:35:24.66 delay 5 seconds @15:35:29.68 PSV.dial PTV @15:35:30.44 delay 5 seconds @15:35:35.46 PSV.dial :air @15:35:36.23 PS.seek 0ml @15:35:58.88 Stroke completed -- waiting for pressure to equalize ... @15:35:58.89 SS.reconfigure :exhale @15:35:58.94 SS.hold @15:35:59.44 getPMLReagent08 @15:35:59.45 PRV.dial :air @15:35:59.92 delay 5 seconds @15:36:04.93 PTV.dial :PRVmixing @15:36:06.25 delay 5 seconds @15:36:10.35 Intake.select :close @15:36:10.49 SSV.dial :exhaust @15:36:11.26 Exhaust.select :open @15:36:11.31 PRV.dial :ab2 @15:36:11.37 SS.seek 0ml @15:36:11.78 PTV.dial :PRVmixing @15:36:11.80 PSV.dial :mixloop @15:36:12.26 PS.reconfigure :default @15:36:12.30 PS.seek 0.3ml @15:36:16.37 delay 2 seconds @15:36:18.39 PRV.dial :air @15:36:18.90 PS.seek 0.55ml @15:36:20.24 Exhaust.select :close @15:36:20.35 SSV.dial CBV @15:36:20.37 CBV.dial SSV @15:36:21.15 Allowing 130s for 25.0ml of remaining 600.0ml @15:36:21.16 Intake.select :open @15:36:22.22 addPMLReagent08 @15:36:22.23 PSV.dial :air @15:36:22.70 PS.seek 2ml @15:36:23.32 Intake @15.8psia, vacuum from 10.0 to 13.0psi @15:36:23.34 SS.reconfigure :inhaling 0.70ml/s @15:36:23.38 SS.seek 25ml @15:36:38.37 PSV.dial :PML @15:36:38.84 PTV.dial :mixing @15:36:39.59 PBV.dial :waste1 @15:36:40.01 PS.seek 1.85ml @15:36:42.37 PBV.dial between PSV and waste1 @15:36:42.76 PS.seek 0ml @15:37:01.46 Stroke completed -- waiting for pressure to equalize ... @15:37:01.47 SS.reconfigure :exhale @15:37:01.51 SS.hold @15:37:02.69 delay 2 seconds @15:37:04.70 delay 8:00 @15:37:14.15 Intake.select :close @15:37:14.29 SSV.dial :exhaust @15:37:15.05 Exhaust.select :open @15:37:15.16 SS.seek 0ml @15:37:23.97 Exhaust.select :close @15:37:24.08 SSV.dial CBV @15:37:24.10 CBV.dial SSV @15:37:24.88 Allowing 130s for 25.0ml of remaining 575.0ml @15:37:24.89 Intake.select :open @15:37:27.05 Intake @15.8psia, vacuum from 10.0 to 13.0psi @15:37:27.07 SS.reconfigure :inhaling 0.70ml/s @15:37:27.11 SS.seek 25ml @15:38:05.14 Stroke completed -- waiting for pressure to equalize ... @15:38:05.16 SS.reconfigure :exhale @15:38:05.20 SS.hold @15:38:19.47 Intake.select :close @15:38:19.61 SSV.dial :exhaust @15:38:20.36 Exhaust.select :open @15:38:20.98 SS.seek 0ml @15:38:29.82 Exhaust.select :close @15:38:29.92 SSV.dial CBV @15:38:29.94 CBV.dial SSV @15:38:30.72 Allowing 130s for 25.0ml of remaining 550.0ml @15:38:30.74 Intake.select :open @15:38:32.89 Intake @15.7psia, vacuum from 10.0 to 13.0psi @15:38:32.91 SS.reconfigure :inhaling 0.70ml/s @15:38:32.95 SS.seek 25ml @15:39:10.81 Stroke completed -- waiting for pressure to equalize ... @15:39:10.83 SS.reconfigure :exhale @15:39:10.87 SS.hold @15:39:27.07 Intake.select :close @15:39:27.21 SSV.dial :exhaust @15:39:27.97 Exhaust.select :open @15:39:28.08 SS.seek 0ml @15:39:36.88 Exhaust.select :close @15:39:36.98 SSV.dial CBV @15:39:37.04 CBV.dial SSV @15:39:37.77 Allowing 130s for 25.0ml of remaining 525.0ml @15:39:37.78 Intake.select :open @15:39:39.94 Intake @15.8psia, vacuum from 10.0 to 13.0psi @15:39:39.96 SS.reconfigure :inhaling 0.70ml/s @15:39:40.00 SS.seek 25ml @15:40:12.32 Vacuum too high -- waiting up to 40 seconds @15:40:12.34 SS.reconfigure :exhale @15:40:12.38 SS.hold @15:40:18.89 Intake @14.8psia, vacuum from 9.3 to 12.3psi @15:40:18.91 SS.reconfigure :inhaling 0.63ml/s @15:40:18.95 SS.seek 25ml @15:40:25.64 Stroke completed -- waiting for pressure to equalize ... @15:40:25.65 SS.reconfigure :exhale @15:40:25.70 SS.hold @15:40:35.37 Intake.select :close @15:40:35.51 SSV.dial :exhaust @15:40:36.26 Exhaust.select :open @15:40:36.37 SS.seek 0ml @15:40:45.39 Exhaust.select :close @15:40:45.50 SSV.dial CBV @15:40:45.52 CBV.dial SSV @15:40:46.28 Allowing 130s for 25.0ml of remaining 500.0ml @15:40:46.30 Intake.select :open @15:40:48.45 Intake @15.8psia, vacuum from 10.0 to 13.0psi @15:40:48.47 SS.reconfigure :inhaling 0.63ml/s @15:40:48.51 SS.seek 25ml @15:41:29.98 Stroke completed -- waiting for pressure to equalize ... @15:41:29.99 SS.reconfigure :exhale @15:41:30.04 SS.hold @15:41:44.81 Intake.select :close @15:41:44.96 SSV.dial :exhaust @15:41:45.70 Exhaust.select :open @15:41:45.81 SS.seek 0ml @15:41:54.67 Exhaust.select :close @15:41:54.78 SSV.dial CBV @15:41:54.80 CBV.dial SSV @15:41:55.56 Allowing 130s for 25.0ml of remaining 475.0ml @15:41:55.58 Intake.select :open @15:41:57.73 Intake @15.8psia, vacuum from 10.0 to 13.0psi @15:41:57.75 SS.reconfigure :inhaling 0.63ml/s @15:41:57.80 SS.seek 25ml @15:42:33.44 Vacuum too high -- waiting up to 40 seconds @15:42:33.46 SS.reconfigure :exhale @15:42:33.50 SS.hold @15:42:40.97 Intake @15.6psia, vacuum from 10.0 to 13.0psi @15:42:40.99 SS.reconfigure :inhaling 0.57ml/s @15:42:41.03 SS.seek 25ml @15:42:48.28 Stroke completed -- waiting for pressure to equalize ... @15:42:48.29 SS.reconfigure :exhale @15:42:48.33 SS.hold @15:42:58.09 Intake.select :close @15:42:58.23 SSV.dial :exhaust @15:42:58.97 Exhaust.select :open @15:42:59.08 SS.seek 0ml @15:43:07.84 Exhaust.select :close @15:43:07.95 SSV.dial CBV @15:43:07.97 CBV.dial SSV @15:43:08.73 Allowing 130s for 25.0ml of remaining 450.0ml @15:43:08.75 Intake.select :open @15:43:10.90 Intake @15.9psia, vacuum from 10.0 to 13.0psi @15:43:10.92 SS.reconfigure :inhaling 0.57ml/s @15:43:10.96 SS.seek 25ml @15:43:57.06 Stroke completed -- waiting for pressure to equalize ... @15:43:57.07 SS.reconfigure :exhale @15:43:57.11 SS.hold @15:44:13.42 Intake.select :close @15:44:13.56 SSV.dial :exhaust @15:44:14.31 Exhaust.select :open @15:44:14.42 SS.seek 0ml @15:44:23.23 Exhaust.select :close @15:44:23.34 SSV.dial CBV @15:44:23.87 CBV.dial SSV @15:44:24.64 Allowing 130s for 25.0ml of remaining 425.0ml @15:44:24.65 Intake.select :open @15:44:26.81 Intake @15.8psia, vacuum from 10.0 to 13.0psi @15:44:26.83 SS.reconfigure :inhaling 0.57ml/s @15:44:26.88 SS.seek 25ml @15:45:04.72 recoverPRVreagent08 @15:45:04.73 PTV.dial :mixing @15:45:04.74 PBV.dial :waste1 @15:45:05.12 delay 30 seconds @15:45:12.93 Stroke completed -- waiting for pressure to equalize ... @15:45:12.94 SS.reconfigure :exhale @15:45:12.99 SS.hold @15:45:31.23 Intake.select :close @15:45:31.37 SSV.dial :exhaust @15:45:32.12 Exhaust.select :open @15:45:32.23 SS.seek 0ml @15:45:35.13 PBV.dial between PSV and waste1 @15:45:35.51 PSV.dial :air @15:45:35.98 PS.reconfigure :fast @15:45:36.02 PS.seek 2.5ml @15:45:41.04 Exhaust.select :close @15:45:41.15 SSV.dial CBV @15:45:41.17 CBV.dial SSV @15:45:41.95 Allowing 130s for 25.0ml of remaining 400.0ml @15:45:41.96 Intake.select :open @15:45:44.12 Intake @16.0psia, vacuum from 10.0 to 13.0psi @15:45:44.14 SS.reconfigure :inhaling 0.57ml/s @15:45:44.18 SS.seek 25ml @15:46:00.08 PSV.dial :mixloop @15:46:00.55 delay 2 seconds @15:46:02.57 PSV.dial :air @15:46:03.04 delay 2 seconds @15:46:05.05 PSV.dial :mixloop @15:46:05.52 delay 2 seconds @15:46:07.54 PSV.dial :air @15:46:08.01 PS.seek 0ml @15:46:15.82 Vacuum too high -- waiting up to 40 seconds @15:46:15.83 SS.reconfigure :exhale @15:46:15.88 SS.hold @15:46:21.41 Intake @15.2psia, vacuum from 9.7 to 12.7psi @15:46:21.43 SS.reconfigure :inhaling 0.51ml/s @15:46:21.47 SS.seek 25ml @15:46:31.37 washCycleDA #1 of 3 @15:46:31.38 PSR.select :air @15:46:31.39 PSV.dial PSR @15:46:32.76 Pulling 0.5ml of PSR.wash @15:46:32.77 PSR.select :wash @15:46:32.89 PS.reconfigure :default @15:46:32.93 PS.seek 0.5ml @15:46:37.88 Stroke completed -- waiting for pressure to equalize ... @15:46:37.90 SS.reconfigure :exhale @15:46:37.94 SS.hold @15:46:38.80 delay 5 seconds @15:46:43.82 PSR.select :air @15:46:43.93 PS.seek 2ml @15:46:50.50 Intake.select :close @15:46:50.64 SSV.dial :exhaust @15:46:51.90 Exhaust.select :open @15:46:52.01 SS.seek 0ml @15:47:00.11 PTV.dial :puck @15:47:00.86 PSV.dial PTV @15:47:01.12 Exhaust.select :close @15:47:01.23 SSV.dial CBV @15:47:01.27 CBV.dial SSV @15:47:01.61 PBV.dial :waste1 @15:47:02.07 PS.seek 1.5ml @15:47:02.09 Allowing 130s for 25.0ml of remaining 375.0ml @15:47:02.12 Intake.select :open @15:47:04.30 Intake @15.9psia, vacuum from 10.0 to 13.0psi @15:47:04.32 SS.reconfigure :inhaling 0.51ml/s @15:47:04.36 SS.seek 25ml @15:47:08.11 delay 3 seconds @15:47:11.13 PBV.dial between PSV and waste1 @15:47:11.51 PS.seek 0ml @15:47:27.68 delay 2:00 @15:47:55.10 Stroke completed -- waiting for pressure to equalize ... @15:47:55.11 SS.reconfigure :exhale @15:47:55.16 SS.hold @15:48:13.44 Intake.select :close @15:48:13.59 SSV.dial :exhaust @15:48:14.35 Exhaust.select :open @15:48:14.46 SS.seek 0ml @15:48:23.30 Exhaust.select :close @15:48:23.41 SSV.dial CBV @15:48:23.43 CBV.dial SSV @15:48:24.21 Allowing 130s for 25.0ml of remaining 350.0ml @15:48:24.23 Intake.select :open @15:48:26.38 Intake @15.8psia, vacuum from 10.0 to 13.0psi @15:48:26.40 SS.reconfigure :inhaling 0.51ml/s @15:48:26.44 SS.seek 25ml @15:49:17.44 Stroke completed -- waiting for pressure to equalize ... @15:49:17.45 SS.reconfigure :exhale @15:49:17.50 SS.hold @15:49:27.69 recoverPSRreagent08 @15:49:27.70 PTV.dial :puck @15:49:27.71 PBV.dial :waste1 @15:49:28.09 delay 30 seconds @15:49:35.72 Intake.select :close @15:49:35.87 SSV.dial :exhaust @15:49:36.63 Exhaust.select :open @15:49:36.74 SS.seek 0ml @15:49:45.79 Exhaust.select :close @15:49:45.90 SSV.dial CBV @15:49:45.93 CBV.dial SSV @15:49:46.68 Allowing 130s for 25.0ml of remaining 325.0ml @15:49:46.69 Intake.select :open @15:49:48.85 Intake @16.0psia, vacuum from 10.0 to 13.0psi @15:49:48.87 SS.reconfigure :inhaling 0.51ml/s @15:49:48.91 SS.seek 25ml @15:49:58.11 PBV.dial between PSV and waste1 @15:49:58.47 PSR.select :air @15:49:58.49 PSV.dial PSR @15:49:59.26 PS.reconfigure :fast @15:49:59.30 PS.seek 2.5ml @15:50:23.17 PSV.dial PTV @15:50:23.94 delay 5 seconds @15:50:28.13 Vacuum too high -- waiting up to 40 seconds @15:50:28.14 SS.reconfigure :exhale @15:50:28.70 SS.hold @15:50:29.02 PSV.dial PSR @15:50:29.78 delay 5 seconds @15:50:34.80 PSV.dial PTV @15:50:35.56 delay 5 seconds @15:50:36.19 Intake @15.6psia, vacuum from 10.0 to 13.0psi @15:50:36.21 SS.reconfigure :inhaling 0.47ml/s @15:50:36.26 SS.seek 25ml @15:50:40.58 PSV.dial :air @15:50:41.35 PS.seek 0ml @15:50:48.88 Stroke completed -- waiting for pressure to equalize ... @15:50:48.89 SS.reconfigure :exhale @15:50:48.93 SS.hold @15:51:03.19 Intake.select :close @15:51:03.33 SSV.dial :exhaust @15:51:04.10 Exhaust.select :open @15:51:04.21 SS.seek 0ml @15:51:04.58 washCycleDA #2 of 3 @15:51:04.59 PSR.select :air @15:51:04.61 PSV.dial PSR @15:51:05.97 Pulling 0.5ml of PSR.wash @15:51:05.98 PSR.select :wash @15:51:06.10 PS.reconfigure :default @15:51:06.14 PS.seek 0.5ml @15:51:12.08 delay 5 seconds @15:51:13.00 Exhaust.select :close @15:51:13.11 SSV.dial CBV @15:51:13.13 CBV.dial SSV @15:51:13.91 Allowing 130s for 25.0ml of remaining 300.0ml @15:51:13.93 Intake.select :open @15:51:16.08 Intake @15.9psia, vacuum from 10.0 to 13.0psi @15:51:16.10 SS.reconfigure :inhaling 0.47ml/s @15:51:16.14 SS.seek 25ml @15:51:17.09 PSR.select :air @15:51:17.21 PS.seek 2ml @15:51:33.32 PTV.dial :puck @15:51:33.34 PSV.dial PTV @15:51:34.08 PBV.dial :waste1 @15:51:34.51 PS.seek 1.5ml @15:51:40.45 delay 3 seconds @15:51:43.47 PBV.dial between PSV and waste1 @15:51:43.85 PS.seek 0ml @15:52:00.08 delay 2:00 @15:52:11.85 Stroke completed -- waiting for pressure to equalize ... @15:52:11.86 SS.reconfigure :exhale @15:52:11.90 SS.hold @15:52:32.21 Intake.select :close @15:52:32.35 SSV.dial :exhaust @15:52:33.11 Exhaust.select :open @15:52:33.22 SS.seek 0ml @15:52:42.09 Exhaust.select :close @15:52:42.72 SSV.dial CBV @15:52:42.74 CBV.dial SSV @15:52:43.52 Allowing 130s for 25.0ml of remaining 275.0ml @15:52:43.54 Intake.select :open @15:52:45.69 Intake @16.0psia, vacuum from 10.0 to 13.0psi @15:52:45.71 SS.reconfigure :inhaling 0.47ml/s @15:52:45.75 SS.seek 25ml @15:53:28.24 Vacuum too high -- waiting up to 40 seconds @15:53:28.26 SS.reconfigure :exhale @15:53:28.30 SS.hold @15:53:35.82 Intake @15.3psia, vacuum from 9.8 to 12.8psi @15:53:35.84 SS.reconfigure :inhaling 0.43ml/s @15:53:35.88 SS.seek 25ml @15:53:51.02 Stroke completed -- waiting for pressure to equalize ... @15:53:51.03 SS.reconfigure :exhale @15:53:51.07 SS.hold @15:54:00.10 recoverPSRreagent08 @15:54:00.11 PTV.dial :puck @15:54:00.12 PBV.dial :waste1 @15:54:00.48 delay 30 seconds @15:54:05.35 Intake.select :close @15:54:05.49 SSV.dial :exhaust @15:54:06.24 Exhaust.select :open @15:54:06.35 SS.seek 0ml @15:54:15.17 Exhaust.select :close @15:54:15.28 SSV.dial CBV @15:54:15.30 CBV.dial SSV @15:54:16.06 Allowing 130s for 25.0ml of remaining 250.0ml @15:54:16.08 Intake.select :open @15:54:18.23 Intake @15.9psia, vacuum from 10.0 to 13.0psi @15:54:18.25 SS.reconfigure :inhaling 0.43ml/s @15:54:18.29 SS.seek 25ml @15:54:30.51 PBV.dial between PSV and waste1 @15:54:30.87 PSR.select :air @15:54:30.89 PSV.dial PSR @15:54:31.66 PS.reconfigure :fast @15:54:31.70 PS.seek 2.5ml @15:54:55.38 PSV.dial PTV @15:54:56.15 delay 5 seconds @15:55:01.17 PSV.dial PSR @15:55:01.93 delay 5 seconds @15:55:06.95 PSV.dial PTV @15:55:07.71 delay 5 seconds @15:55:12.73 PSV.dial :air @15:55:13.61 PS.seek 0ml @15:55:20.14 Stroke completed -- waiting for pressure to equalize ... @15:55:20.15 SS.reconfigure :exhale @15:55:20.19 SS.hold @15:55:36.79 washCycleDA #3 of 3 @15:55:36.80 PSR.select :air @15:55:36.82 PSV.dial PSR @15:55:38.19 Pulling 0.5ml of PSR.wash @15:55:38.20 PSR.select :wash @15:55:38.31 PS.reconfigure :default @15:55:38.35 PS.seek 0.5ml @15:55:41.11 Intake.select :close @15:55:41.25 SSV.dial :exhaust @15:55:42.00 Exhaust.select :open @15:55:42.11 SS.seek 0ml @15:55:44.33 delay 5 seconds @15:55:49.34 PSR.select :air @15:55:49.45 PS.seek 2ml @15:55:50.90 Exhaust.select :close @15:55:51.01 SSV.dial CBV @15:55:51.03 CBV.dial SSV @15:55:51.80 Allowing 130s for 25.0ml of remaining 225.0ml @15:55:51.81 Intake.select :open @15:55:53.97 Intake @16.0psia, vacuum from 10.0 to 13.0psi @15:55:53.98 SS.reconfigure :inhaling 0.43ml/s @15:55:54.03 SS.seek 25ml @15:56:05.54 PTV.dial :puck @15:56:05.55 PSV.dial PTV @15:56:06.30 PBV.dial :waste1 @15:56:06.72 PS.seek 1.5ml @15:56:12.60 delay 3 seconds @15:56:15.62 PBV.dial between PSV and waste1 @15:56:16.00 PS.seek 0ml @15:56:32.13 delay 2:00 @15:56:48.21 Vacuum too high -- waiting up to 40 seconds @15:56:48.22 SS.reconfigure :exhale @15:56:48.26 SS.hold @15:56:58.05 Intake @15.8psia, vacuum from 10.0 to 13.0psi @15:56:58.07 SS.reconfigure :inhaling 0.38ml/s @15:56:58.11 SS.seek 25ml @15:57:06.78 Stroke completed -- waiting for pressure to equalize ... @15:57:06.79 SS.reconfigure :exhale @15:57:06.83 SS.hold @15:57:21.12 Intake.select :close @15:57:21.26 SSV.dial :exhaust @15:57:22.00 Exhaust.select :open @15:57:22.11 SS.seek 0ml @15:57:31.15 Exhaust.select :close @15:57:31.26 SSV.dial CBV @15:57:31.28 CBV.dial SSV @15:57:32.04 Allowing 130s for 25.0ml of remaining 200.0ml @15:57:32.06 Intake.select :open @15:57:34.21 Intake @16.1psia, vacuum from 10.0 to 13.0psi @15:57:34.23 SS.reconfigure :inhaling 0.38ml/s @15:57:34.27 SS.seek 25ml @15:58:32.16 recoverPSRreagent08 @15:58:32.17 PTV.dial :puck @15:58:32.18 PBV.dial :waste1 @15:58:32.55 delay 30 seconds @15:58:42.96 Stroke completed -- waiting for pressure to equalize ... @15:58:42.97 SS.reconfigure :exhale @15:58:43.02 SS.hold @15:59:02.57 PBV.dial between PSV and waste1 @15:59:02.94 PSR.select :air @15:59:02.96 PSV.dial PSR @15:59:03.39 Intake.select :close @15:59:03.53 SSV.dial :exhaust @15:59:03.73 PS.reconfigure :fast @15:59:03.78 PS.seek 2.5ml @15:59:04.31 Exhaust.select :open @15:59:04.42 SS.seek 0ml @15:59:13.18 Exhaust.select :close @15:59:13.29 SSV.dial CBV @15:59:13.31 CBV.dial SSV @15:59:14.09 Allowing 130s for 25.0ml of remaining 175.0ml @15:59:14.10 Intake.select :open @15:59:16.26 Intake @16.0psia, vacuum from 10.0 to 13.0psi @15:59:16.28 SS.reconfigure :inhaling 0.38ml/s @15:59:16.32 SS.seek 25ml @15:59:27.47 PSV.dial PTV @15:59:28.24 delay 5 seconds @15:59:33.26 PSV.dial PSR @15:59:34.02 delay 5 seconds @15:59:39.04 PSV.dial PTV @15:59:39.80 delay 5 seconds @15:59:44.82 PSV.dial :air @15:59:45.59 PS.seek 0ml @16:00:08.85 Gate.power :camera,:ON @16:00:08.86 Mixing DA Substrates @16:00:08.87 PSR.select :air @16:00:08.89 PSV.dial PSR @16:00:10.40 Pulling 0.25ml of PSR.sub1 @16:00:10.43 PSR.select :sub1 @16:00:10.62 PS.reconfigure :default @16:00:10.74 PS.seek 0.25ml @16:00:14.20 delay 5 seconds @16:00:19.27 Pulling 0.25ml of PSR.sub2 @16:00:19.28 PSR.select :sub2 @16:00:19.39 PS.seek 0.5ml @16:00:22.79 delay 5 seconds @16:00:25.66 Stroke completed -- waiting for pressure to equalize ... @16:00:25.68 SS.reconfigure :exhale @16:00:25.72 SS.hold @16:00:27.80 PSR.select :air @16:00:27.96 PS.seek 2ml @16:00:44.16 PTV.dial :puck @16:00:44.17 PSV.dial PTV @16:00:44.92 PBV.dial :waste1 @16:00:45.36 PS.seek 1.5ml @16:00:50.12 Intake.select :close @16:00:50.27 SSV.dial :exhaust @16:00:51.02 Exhaust.select :open @16:00:51.14 SS.seek 0ml @16:00:51.28 delay 3 seconds @16:00:54.30 PBV.dial between PSV and waste1 @16:00:54.70 PS.seek 0ml @16:01:00.01 Exhaust.select :close @16:01:00.11 SSV.dial CBV @16:01:00.17 CBV.dial SSV @16:01:00.92 Allowing 130s for 25.0ml of remaining 150.0ml @16:01:00.93 Intake.select :open @16:01:03.08 Intake @16.1psia, vacuum from 10.0 to 13.0psi @16:01:03.10 SS.reconfigure :inhaling 0.38ml/s @16:01:03.14 SS.seek 25ml @16:01:10.78 delay 10 seconds @16:01:20.81 recoverPSRreagent08 @16:01:20.82 PTV.dial :puck @16:01:20.83 PBV.dial :waste1 @16:01:21.22 delay 30 seconds @16:01:51.25 PBV.dial between PSV and waste1 @16:01:51.63 PSR.select :air @16:01:51.65 PSV.dial PSR @16:01:52.43 PS.reconfigure :fast @16:01:52.48 PS.seek 2.5ml @16:02:04.67 Vacuum too high -- waiting up to 40 seconds @16:02:04.68 SS.reconfigure :exhale @16:02:04.72 SS.hold @16:02:16.01 Intake @16.0psia, vacuum from 10.0 to 13.0psi @16:02:16.03 SS.reconfigure :inhaling 0.34ml/s @16:02:16.07 SS.seek 25ml @16:02:16.44 PSV.dial PTV @16:02:17.22 delay 5 seconds @16:02:22.24 PSV.dial PSR @16:02:23.00 delay 5 seconds @16:02:25.18 Stroke completed -- waiting for pressure to equalize ... @16:02:25.19 SS.reconfigure :exhale @16:02:25.23 SS.hold @16:02:28.08 PSV.dial PTV @16:02:28.85 delay 5 seconds @16:02:33.86 PSV.dial :air @16:02:34.65 PS.seek 0ml @16:02:41.42 Intake.select :close @16:02:41.56 SSV.dial :exhaust @16:02:42.32 Exhaust.select :open @16:02:42.43 SS.seek 0ml @16:02:51.29 Exhaust.select :close @16:02:51.39 SSV.dial CBV @16:02:51.41 CBV.dial SSV @16:02:52.18 Allowing 130s for 25.0ml of remaining 125.0ml @16:02:52.19 Intake.select :open @16:02:54.35 Intake @16.0psia, vacuum from 10.0 to 13.0psi @16:02:54.36 SS.reconfigure :inhaling 0.34ml/s @16:02:54.41 SS.seek 25ml @16:02:58.38 Additional air clear @16:02:58.39 PSR.select :air @16:02:58.40 PSV.dial PSR @16:02:59.79 Pulling 2ml of PSR.air @16:02:59.80 PSR.select :air @16:02:59.82 PS.reconfigure :default @16:02:59.86 PS.seek 2ml @16:03:21.06 delay 5 seconds @16:03:26.08 PTV.dial :puck @16:03:26.10 PSV.dial PTV @16:03:26.84 PBV.dial :waste1 @16:03:27.29 PS.seek 1.5ml @16:03:33.19 delay 3 seconds @16:03:36.21 PBV.dial between PSV and waste1 @16:03:36.60 delay 1 second @16:03:37.61 PSV.dial :waste1 @16:03:38.68 PS.seek 0ml @16:03:54.89 Processing Puck Heater at 28C before imaging @16:03:54.90 PH.coast @16:03:55.03 Imaging da puck in Processing Clamp while storing it to tube 2 @16:03:55.19 SC.reconfigure :tight @16:03:55.24 SC.seek 2 @16:03:58.81 SC.configure :limp @16:03:59.01 Elbow.reconfigure :tight @16:03:59.06 Elbow.seek 2 @16:04:00.80 Forearm.reconfigure :tight @16:04:00.85 Forearm.seek 2 + 450 counts @16:04:01.83 SE.reconfigure :pick @16:04:01.88 SE.seek :beamBreak @16:04:04.77 SE.configure :default @16:04:04.92 Elbow.reconfigure :limp @16:04:04.96 Forearm.reconfigure :loose @16:04:05.00 Forearm.seek 2 @16:04:05.33 Elbow.hold @16:04:05.39 Elbow.reconfigure :tight @16:04:05.48 SE.seek 4.17 Pucks below Top Plate @16:04:05.92 Forearm.force -20 @16:04:05.96 Forearm.seek 2 + 287 counts @16:04:06.76 Forearm.reconfigure :tight @16:04:06.80 Forearm.seek 2 + 450 counts @16:04:07.04 SE.seek 5.17 Pucks below Top Plate @16:04:07.67 Forearm.reconfigure :loose @16:04:07.71 Forearm.seek :retract @16:04:08.62 Elbow.seek PC @16:04:11.15 Elbow.reconfigure :loose @16:04:11.24 Forearm.seek PC @16:04:12.20 Hand.close @16:04:12.87 PC.seek :open @16:04:14.80 Stroke completed -- waiting for pressure to equalize ... @16:04:14.81 SS.reconfigure :exhale @16:04:14.86 SS.hold @16:04:17.66 Forearm.seek :retract @16:04:18.66 Elbow.reconfigure :tight @16:04:18.71 Elbow.seek 2 @16:04:21.39 Forearm.reconfigure :tight @16:04:21.48 Forearm.seek 2 + 450 counts @16:04:22.56 Hand.open @16:04:23.17 SE.seek 4.82 Pucks below Top Plate @16:04:23.50 SE.reconfigure :seal @16:04:23.54 SE.seek 3.85 Pucks below Top Plate @16:04:24.11 Lights, camera, ACTION! @16:04:24.14 PuckCamera: snap -bin=2,2 -auto /var/log/esp/da23jan0916h975ml.tif @16:04:37.40 Intake.select :close @16:04:37.54 SSV.dial :exhaust @16:04:38.31 Exhaust.select :open @16:04:38.42 SS.seek 0ml @16:04:42.10 SX HX9/16 (USB:10): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 14.666 seconds /var/log/esp/da23jan0916h975ml.tif: TIFF Upload Complete @16:04:42.13 PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da23jan0916h975ml15s.tif @16:04:43.59 Waiting >20 seconds for da to relinquish FlushPuck @16:04:47.55 Exhaust.select :close @16:04:47.66 SSV.dial CBV @16:04:47.69 CBV.dial SSV @16:04:48.47 Allowing 130s for 25.0ml of remaining 100.0ml @16:04:48.49 Intake.select :open @16:04:50.65 Intake @16.1psia, vacuum from 10.0 to 13.0psi @16:04:50.67 SS.reconfigure :inhaling 0.34ml/s @16:04:50.71 SS.seek 25ml @16:04:57.74 SX HX9/16 (USB:10): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15 seconds /var/log/esp/midres/da23jan0916h975ml15s.tif: TIFF Upload Complete @16:04:57.77 PuckCamera: snap -bin=2,2 20.0 /var/log/esp/midres/da23jan0916h975ml20s.tif @16:05:18.42 SX HX9/16 (USB:10): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 20 seconds /var/log/esp/midres/da23jan0916h975ml20s.tif: TIFF Upload Complete @16:05:18.44 Gate.power :camera,:OFF @16:05:18.46 DAfans.email "14.666 sec auto-exposure",Subject: "da23jan0916h975ml images!" @16:05:18.72 Elbow.seek 2 @16:05:18.90 Forearm.seek 2 + 450 counts @16:05:19.09 SE.reconfigure :gently @16:05:19.14 SE.seek 4.57 Pucks below Top Plate @16:05:19.80 Elbow.reconfigure :limp @16:05:19.85 Forearm.reconfigure :loose @16:05:19.89 Forearm.seek 2 @16:05:20.17 Elbow.hold @16:05:20.25 Elbow.reconfigure :tight @16:05:20.30 SE.reconfigure :default @16:05:20.35 SE.seek 4.75 Pucks below Top Plate @16:05:21.37 Forearm.force -20 @16:05:21.42 Forearm.seek 2 + 286 counts @16:05:21.74 Forearm.reconfigure :tight @16:05:21.79 Forearm.seek 2 + 450 counts @16:05:22.06 SE.seek :down @16:05:23.18 Forearm.reconfigure :loose @16:05:23.23 Forearm.seek :retract @16:05:24.49 SE.stop @16:05:24.61 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @16:05:24.94 Elbow.seek FlushPuck::Garage @16:05:26.96 Elbow.reconfigure :loose @16:05:27.15 Forearm.seek :garage @16:05:28.15 Hand.close @16:05:29.00 Forearm.seek :retract @16:05:30.01 Elbow.reconfigure :tight @16:05:30.12 Elbow.seek PC @16:05:31.47 Elbow.reconfigure :loose @16:05:31.66 Forearm.reconfigure :tight @16:05:31.75 Forearm.seek PC @16:05:33.39 PC.seek :closed @16:05:38.19 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.58 @16:05:38.33 Hand.open @16:05:39.15 Forearm.reconfigure :loose @16:05:39.25 Forearm.seek :retract @16:05:40.31 PSR.select :air @16:05:40.35 PSV.dial PSR @16:05:40.97 Pulling 0.5ml of PSR.flush @16:05:40.99 PSR.select :flush @16:05:41.17 PS.seek 0.5ml @16:05:47.09 delay 5 seconds @16:05:52.13 PSR.select :air @16:05:52.29 PS.seek 2.5ml @16:05:53.90 upsync @16:06:11.28 Stroke completed -- waiting for pressure to equalize ... @16:06:11.30 SS.reconfigure :exhale @16:06:11.34 SS.hold @16:06:13.53 delay 1 second @16:06:14.55 PRV.dial :waste2,avoiding: :ab2 @16:06:16.42 PTV.dial :PRVmixing @16:06:17.73 PSV.dial :mixloop @16:06:18.77 PS.seek :empty @16:06:35.71 Intake.select :close @16:06:35.86 SSV.dial :exhaust @16:06:36.63 Exhaust.select :open @16:06:36.74 SS.seek 0ml @16:06:45.15 PSR.select :air @16:06:45.17 PSV.dial PSR @16:06:45.55 Exhaust.select :close @16:06:45.66 SSV.dial CBV @16:06:45.69 CBV.dial SSV @16:06:46.81 Pulling 0.5ml of PSR.flush @16:06:46.82 PSR.select :flush @16:06:46.84 Allowing 130s for 25.0ml of remaining 75.0ml @16:06:46.88 Intake.select :open @16:06:46.98 PS.seek 0.5ml @16:06:49.08 Intake @16.1psia, vacuum from 10.0 to 13.0psi @16:06:49.10 SS.reconfigure :inhaling 0.34ml/s @16:06:49.14 SS.seek 25ml @16:06:52.94 delay 5 seconds @16:06:57.96 PSR.select :air @16:06:58.07 PS.seek 2.5ml @16:07:19.34 delay 1 second @16:07:20.36 PBV.dial :waste1 @16:07:20.73 PTV.dial :mixing @16:07:21.48 PSV.dial :mixloop @16:07:22.55 PS.seek :empty @16:07:49.09 PSV.dial PSR @16:07:50.13 PSR.select :flush @16:07:50.25 PS.seek 1ml @16:08:01.21 PSR.select :air @16:08:01.33 PS.seek 2.5ml @16:08:10.65 Stroke completed -- waiting for pressure to equalize ... @16:08:10.67 SS.reconfigure :exhale @16:08:10.71 SS.hold @16:08:17.49 PSV.dial PTV @16:08:18.37 PTV.dial :puck @16:08:19.14 PBV.dial :waste1 @16:08:19.16 PS.seek 0ml @16:08:39.16 Intake.select :close @16:08:39.30 SSV.dial :exhaust @16:08:40.06 Exhaust.select :open @16:08:40.17 SS.seek 0ml @16:08:45.46 PSV.dial PSR @16:08:46.21 PSR.select :flush @16:08:46.33 PS.seek 0.5ml @16:08:49.25 Exhaust.select :close @16:08:49.36 SSV.dial CBV @16:08:49.39 CBV.dial SSV @16:08:50.16 Allowing 130s for 25.0ml of remaining 50.0ml @16:08:50.17 Intake.select :open @16:08:52.21 PSR.select :air @16:08:52.32 Intake @16.2psia, vacuum from 10.0 to 13.0psi @16:08:52.34 SS.reconfigure :inhaling 0.34ml/s @16:08:52.38 SS.seek 25ml @16:08:52.44 PS.seek 2.5ml @16:09:13.74 PSV.dial PTV @16:09:14.51 PBV.dial PSV @16:09:15.08 PS.seek 0ml @16:09:41.46 PSV.dial PBV @16:09:41.94 PS.seek 2.5ml @16:09:58.57 Vacuum too high -- waiting up to 40 seconds @16:09:58.58 SS.reconfigure :exhale @16:09:58.62 SS.hold @16:10:08.35 PSV.dial :waste1 @16:10:09.11 PS.seek 0ml @16:10:11.27 Intake @15.9psia, vacuum from 10.0 to 13.0psi @16:10:11.29 SS.reconfigure :inhaling 0.31ml/s @16:10:11.83 SS.seek 25ml @16:10:27.35 Stroke completed -- waiting for pressure to equalize ... @16:10:27.37 SS.reconfigure :exhale @16:10:27.41 SS.hold @16:10:35.49 PSV.dial PSR @16:10:35.96 PSR.select :air @16:10:35.98 PS.seek 2.5ml @16:10:47.69 Intake.select :close @16:10:47.84 SSV.dial :exhaust @16:10:48.61 Exhaust.select :open @16:10:48.72 SS.seek 0ml @16:10:57.70 Exhaust.select :close @16:10:57.81 SSV.dial CBV @16:10:57.84 CBV.dial SSV @16:10:58.61 Allowing 130s for 25.0ml of remaining 25.0ml @16:10:58.62 Intake.select :open @16:11:00.77 Intake @16.3psia, vacuum from 10.0 to 13.0psi @16:11:00.79 SS.reconfigure :inhaling 0.31ml/s @16:11:00.83 SS.seek 24.96ml @16:11:02.33 PSV.dial PTV @16:11:03.20 PBV.dial PSV @16:11:03.22 PS.seek 0ml @16:11:29.58 PSV.dial PBV @16:11:30.05 PS.seek 2.5ml @16:11:56.46 PSV.dial :waste1 @16:11:57.22 PS.seek 0ml @16:12:23.49 PSV.dial PSR @16:12:23.96 PSR.select :flush @16:12:24.08 PS.seek 1ml @16:12:24.69 Vacuum too high -- waiting up to 40 seconds @16:12:24.70 SS.reconfigure :exhale @16:12:24.74 SS.hold @16:12:35.20 PSR.select :air @16:12:35.32 PS.seek 2.5ml @16:12:39.15 Intake @16.3psia, vacuum from 10.0 to 13.0psi @16:12:39.17 SS.reconfigure :inhaling 0.28ml/s @16:12:39.21 SS.seek 24.96ml @16:12:44.01 Stroke completed -- waiting for pressure to equalize ... @16:12:44.02 SS.reconfigure :exhale @16:12:44.06 SS.hold @16:12:51.55 PSV.dial PTV @16:12:52.41 PTV.dial :puck @16:12:52.43 PBV.dial :waste1 @16:12:52.98 PS.seek 0ml @16:13:00.87 Intake.select :close @16:13:01.01 SSV.dial :exhaust @16:13:01.78 Exhaust.select :open @16:13:01.89 SS.seek 0ml @16:13:10.71 Exhaust.select :close @16:13:10.82 Sampled 1000.0ml @16:13:10.84 Drying puck before fixing for 20:00 @16:13:10.85 CBV.dial :waste2b @16:13:11.59 delay 1:00 @16:13:19.36 PSV.dial PSR @16:13:20.11 PSR.select :flush @16:13:20.23 PS.seek 0.5ml @16:13:26.17 PSR.select :air @16:13:26.29 PS.seek 2.5ml @16:13:47.74 PSV.dial PTV @16:13:48.50 PBV.dial PSV @16:13:49.07 PS.seek 0ml @16:14:11.62 CSV.dial :air @16:14:12.08 CS.reconfigure :fast @16:14:12.13 CS.seek 10ml @16:14:15.49 PSV.dial PBV @16:14:15.96 PS.seek 2.5ml @16:14:36.62 Pumping wcr puck from tube 3 up to 35psi... @16:14:36.64 CBV.dial SSV @16:14:37.95 CBV.dial between CSV and waste2b @16:14:38.92 CSV.dial between air and CTV @16:14:39.24 CS.seek 7.058ml @16:14:42.40 PSV.dial :waste1 @16:14:43.14 PS.seek 0ml @16:14:46.66 CTV.dial CSV,avoiding: :air @16:14:46.68 CSV.dial CTV @16:14:47.50 CS.reconfigure :pressurize @16:14:47.55 CS.seek 0ml @16:15:00.95 CBV.dial :waste2b @16:15:01.28 CS.reconfigure :default @16:15:01.33 CS.seek 0ml @16:15:07.15 delay 1:00 @16:15:09.55 PSV.dial PSR @16:15:10.02 PSR.select :air @16:15:10.04 PS.seek 2.5ml @16:15:36.45 PSV.dial PTV @16:15:37.19 PBV.dial PSV @16:15:37.21 PS.seek 0ml @16:16:03.58 PSV.dial PBV @16:16:04.05 PS.seek 2.5ml @16:16:07.17 CTV.dial :air,avoiding: :intake @16:16:08.50 Fix #1 of 2 (with RNAlater) for 10:00 @16:16:08.52 CSV.dial CSR @16:16:09.03 Pulling 2ml of CSR.RNAlater @16:16:09.04 CSR.select :RNAlater @16:16:09.15 CS.seek 2ml @16:16:15.18 delay 5 seconds @16:16:20.20 CSR.select :air @16:16:20.31 CS.reconfigure :fast @16:16:20.36 CS.seek 10ml @16:16:30.42 PSV.dial :waste1 @16:16:31.16 PS.seek 0ml @16:16:40.00 Pumping wcr puck from tube 3 up to 35psi... @16:16:40.01 CBV.dial SSV @16:16:41.33 CBV.dial between CSV and waste2b @16:16:42.30 CSV.dial between air and CTV @16:16:42.90 CS.seek 7.074ml @16:16:50.38 CTV.dial CSV,avoiding: :air @16:16:50.41 CSV.dial CTV @16:16:51.77 CS.reconfigure :pressurize @16:16:51.81 CS.seek 0ml @16:16:57.52 Garaging FlushPuck in Processing Clamp @16:16:57.59 Elbow.reconfigure :tight @16:16:57.63 Elbow.seek PC @16:16:57.75 Elbow.reconfigure :loose @16:16:58.30 Forearm.seek PC @16:16:59.24 Hand.close @16:16:59.86 PC.seek :open @16:17:04.56 Forearm.seek :retract @16:17:05.56 Elbow.reconfigure :tight @16:17:05.60 Elbow.seek :garage @16:17:05.77 delay 10:00 @16:17:06.96 Elbow.reconfigure :loose @16:17:07.05 Forearm.reconfigure :tight @16:17:07.09 Forearm.seek :garage @16:17:08.13 Hand.open @16:17:08.79 Forearm.reconfigure :loose @16:17:08.83 Forearm.seek :retract @16:27:05.79 CBV.dial :waste2b @16:27:06.13 CS.reconfigure :default @16:27:06.18 CS.seek 0ml @16:27:11.55 delay 1:00 @16:28:11.57 CTV.dial :air,avoiding: :intake @16:28:12.91 Fix #2 of 2 (with RNAlater) for 10:00 @16:28:12.92 CSV.dial CSR @16:28:13.41 Pulling 2ml of CSR.RNAlater @16:28:13.42 CSR.select :RNAlater @16:28:13.53 CS.seek 2ml @16:28:19.45 delay 5 seconds @16:28:24.47 CSR.select :air @16:28:24.58 CS.reconfigure :fast @16:28:24.63 CS.seek 10ml @16:28:44.14 Pumping wcr puck from tube 3 up to 35psi... @16:28:44.15 CBV.dial SSV @16:28:45.46 CBV.dial between CSV and waste2b @16:28:46.40 CSV.dial between air and CTV @16:28:47.01 CS.seek 7.033ml @16:28:54.61 CTV.dial CSV,avoiding: :air @16:28:54.64 CSV.dial CTV @16:28:55.99 CS.reconfigure :pressurize @16:28:56.04 CS.seek 0ml @16:29:14.16 delay 10:00 @16:39:14.18 CBV.dial :waste2b @16:39:14.51 CS.reconfigure :default @16:39:14.55 CS.seek 0ml @16:39:15.47 delay 1:00 @16:40:15.49 CTV.dial :air,avoiding: :intake @16:40:16.87 Storing wcr puck in Collection Clamp to tube 2 @16:40:17.03 SC.reconfigure :tight @16:40:17.08 SC.seek 2 @16:40:17.45 SC.configure :limp @16:40:17.65 Elbow.reconfigure :tight @16:40:17.69 Elbow.seek 2 @16:40:19.53 Forearm.reconfigure :tight @16:40:19.58 Forearm.seek 2 + 450 counts @16:40:20.56 SE.reconfigure :pick @16:40:20.62 SE.seek :beamBreak @16:40:23.35 SE.configure :default @16:40:23.54 Elbow.reconfigure :limp @16:40:23.59 Forearm.reconfigure :loose @16:40:23.63 Forearm.seek 2 @16:40:23.91 Elbow.hold @16:40:23.97 Elbow.reconfigure :tight @16:40:24.07 SE.seek 5.16 Pucks below Top Plate @16:40:24.52 Forearm.force -20 @16:40:24.56 Forearm.seek 2 + 285 counts @16:40:24.84 Forearm.reconfigure :tight @16:40:24.88 Forearm.seek 2 + 450 counts @16:40:25.07 SE.seek 6.16 Pucks below Top Plate @16:40:25.57 Forearm.reconfigure :loose @16:40:26.08 Forearm.seek :retract @16:40:26.99 Elbow.seek CC @16:40:28.61 Elbow.reconfigure :loose @16:40:28.70 Forearm.seek CC @16:40:29.67 Hand.close @16:40:30.34 CC.seek :open @16:40:35.07 Forearm.seek :retract @16:40:36.02 Elbow.reconfigure :tight @16:40:36.07 Elbow.seek 2 @16:40:37.85 Elbow.reconfigure :limp @16:40:37.90 Forearm.seek 2 @16:40:38.85 Elbow.hold @16:40:38.92 Elbow.reconfigure :tight @16:40:38.96 Hand.open @16:40:39.70 Forearm.force -20 @16:40:39.74 Forearm.seek 2 + 311 counts @16:40:40.14 Forearm.reconfigure :tight @16:40:40.19 Forearm.seek 2 + 450 counts @16:40:40.45 SE.seek :down @16:40:41.57 Forearm.reconfigure :loose @16:40:41.62 Forearm.seek :retract @16:40:42.73 SE.stop @16:40:42.77
Starting finalPurge @16:40:42.78 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @16:40:42.97 Elbow.seek FlushPuck::Garage @16:40:44.79 Elbow.reconfigure :loose @16:40:44.88 Forearm.seek :garage @16:40:45.88 Hand.close @16:40:46.57 Forearm.seek :retract @16:40:47.54 Elbow.reconfigure :tight @16:40:47.58 Elbow.seek CC @16:40:50.53 Elbow.reconfigure :loose @16:40:50.61 Forearm.reconfigure :tight @16:40:50.66 Forearm.seek CC @16:40:51.90 CC.seek :closed @16:40:56.43 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.31 @16:40:56.50 Hand.open @16:40:57.15 Forearm.reconfigure :loose @16:40:57.19 Forearm.seek :retract @16:40:58.13 Flushing the Collection Valve::Manifold @16:40:58.25 CSV.dial CSR @16:40:58.73 Pulling 5ml of CSR.flush @16:40:58.74 CSR.select :flush @16:40:58.85 CS.seek 5ml @16:41:12.63 delay 5 seconds @16:41:17.64 CSR.select :air @16:41:17.80 CS.reconfigure :fast @16:41:17.84 CS.seek 7.501ml @16:41:24.62 CSV.dial :waste @16:41:25.90 CS.reconfigure :default @16:41:25.94 CS.seek 0ml @16:41:45.91 Flushing lower manifold's waste1 port @16:41:46.02 CSV.dial :flush @16:41:46.46 CS.reconfigure :fast @16:41:46.50 CS.seek 5ml @16:41:59.09 delay 5 seconds @16:42:04.11 CSV.dial :air @16:42:04.61 CS.seek 7.001ml @16:42:10.09 CSV.dial CTV @16:42:10.12
CTV.dial CSV @16:42:11.48 CBV.dial :waste1 @16:42:12.24 CS.seek 0ml @16:42:28.78 Flushing lower manifold's waste2 port @16:42:28.89 CSV.dial :flush @16:42:30.19 CS.seek 5ml @16:42:42.94 delay 5 seconds @16:42:47.96 CSV.dial :air @16:42:48.47 CS.seek 7.001ml @16:42:53.94 CSV.dial CTV @16:42:53.96
CTV.dial CSV @16:42:54.41 CBV.dial :waste2 @16:42:55.46 CS.seek 0ml @16:43:11.97 Flushing lower manifold's waste2b port @16:43:12.08 CSV.dial :flush @16:43:12.82 CS.seek 5ml @16:43:25.59 delay 5 seconds @16:43:30.61 CSV.dial :air @16:43:31.12 CS.seek 7.001ml @16:43:36.84 CSV.dial CTV @16:43:36.86
CTV.dial CSV @16:43:37.31 CBV.dial :waste2b @16:43:38.36 CS.seek 0ml @16:43:54.97 Disinfecting external Intake with kill @16:43:55.08 CSV.dial CSR @16:43:55.57 Pulling 8ml of CSR.kill @16:43:55.58 CSR.select :kill @16:43:55.69 CS.seek 8ml @16:44:15.34 delay 5 seconds @16:44:20.36 CSR.select :air @16:44:20.48 CS.seek 10ml @16:44:26.03 CSV.dial CBV @16:44:26.05
CBV.dial CSV @16:44:27.11 CTV.dial :intake @16:44:27.87 Intake.select :open @16:44:27.98 CS.seek 2ml @16:44:46.71 Intake.select :close @16:44:46.81 CTV.dial :air @16:44:47.58 CS.reconfigure :default @16:44:47.62 CS.seek 0ml @16:44:53.59 CSV.dial CSR @16:44:54.64 Pulling 5ml of CSR.flush @16:44:54.65 CSR.select :flush @16:44:54.76 CS.seek 5ml @16:45:08.56 delay 5 seconds @16:45:13.58 CSR.select :air @16:45:13.69 CS.seek 10ml @16:45:27.24 CSV.dial CBV @16:45:28.24 CS.seek 0ml @16:45:54.68 Garaging FlushPuck in Collection Clamp @16:45:54.75 Elbow.reconfigure :tight @16:45:54.79 Elbow.seek CC @16:45:55.18 Elbow.reconfigure :loose @16:45:55.27 Forearm.seek CC @16:45:56.23 Hand.close @16:45:56.83 CC.seek :open @16:46:01.45 Forearm.seek :retract @16:46:02.49 Elbow.reconfigure :tight @16:46:02.53 Elbow.seek :garage @16:46:05.46 Elbow.reconfigure :loose @16:46:05.55 Forearm.reconfigure :tight @16:46:05.59 Forearm.seek :garage @16:46:06.58 Hand.open @16:46:07.26 Forearm.reconfigure :loose @16:46:07.30 Forearm.seek :retract @16:46:08.27 Completed [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] begun 13:41:04 @16:46:08.32 Asleep after completing DA_WCR_1923 assays @16:46:08.33 GoodNews.email "{1 => 33,\n 2 => 7,\n 3 => 26,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@16:40:13, 17% flow, 17.5C, 30% humidity, 14.7psia, 13.734V, 0.429A, 0.411A avg, 5.90W\nSampled 1000.0ml",Subject: "Asleep after completing DA_WCR_1923 assays" @16:46:13.81 upsync @15:01:45.65UTC17-Jan-23
ESP2 v3.41 I2C Gateway v3.20 for core bus @15:01:48.23 Configuration for short puck jake 1/11/21 by brent@mbari.org /home/esp/esp2/type/shallow/jake/configure.rb @15:01:52.20 Polling Can every 10:00 @15:01:52.31 Cleanly exited at 14:59:25UTC17-Jan-23 @15:01:52.34 Loading /home/esp/esp2/mission/DA_WCR_11723.rb mission @15:01:52.40 GoodNews.email "Starting DA_WCR_11723 w/pucks in tube 3\n{1 => 33,\n 2 => 7,\n 3 => 26,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@15:01:52, 19% flow, 14.2C, 31% humidity, 14.5psia, 13.769V, 0.327A, 0.315A avg, 4.50W",Subject: "DA_WCR_11723 begun" @15:01:52.44
Preparing to sleep until + 00:00:05 @15:01:52.46 Asleep til + 00:00:05 @15:01:52.48 Continuing at 15:01 @15:01:52.77 Gate.power :core,:OFF @15:01:52.78 Gate.power :raw,:OFF @15:01:52.79 Shutdown @15:01:56.89 GoodNews.email "Then, in tube 3: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2/mission/DA_WCR_11723.rb:14\n{1 => 33,\n 2 => 7,\n 3 => 26,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@15:01:52, 19% flow, 14.2C, 31% humidity, 14.5psia, 13.769V, 0.327A, 0.315A avg, 4.50W",Subject: "Asleep until 15:01" @15:01:57.39
Powering core bus @15:01:58.63 upsync @15:02:02.13 upsync @15:02:03.10
Gate.power :raw,:ON @15:02:03.78 CS.configure :default @15:02:03.87 PS.configure :default @15:02:03.96 SS.configure :default @15:02:04.05 CC.configure :default @15:02:04.14 PC.configure :default @15:02:04.25 Forearm.configure :loose @15:02:04.33 Elbow.configure :loose @15:02:04.44 SC.configure :loose @15:02:04.52 SE.configure :default @15:02:04.56 CH.coast @15:02:04.61 CH.configure :default @15:02:04.64 PH.coast @15:02:04.70 PH.configure :default @15:02:04.73 All dwarf controllers running firmware version 3.85 @15:02:04.83 Valve.reset @15:02:05.04 Intake.select :close @15:02:05.09 Exhaust.select :close @15:02:05.15 CTV.dial :air @15:02:05.24 PSV.dial :air @15:02:05.34 CSV.dial :waste1 @15:02:05.37
Forearm.reconfigure :loose @15:02:05.44 Forearm.home.seek :retract @15:02:05.81 SSV.dial :spit,avoiding: :exhaust @15:02:06.20 Hand.close @15:02:06.23
Elbow.jog 1500 @15:02:06.45 PS.reconfigure :default @15:02:06.50 PS.home.seek 0ml @15:02:06.63 CS.reconfigure :fast @15:02:06.66 SS.reconfigure :default @15:02:06.75 CS.home.seek 0ml @15:02:06.78 SS.home.seek 0ml @15:02:06.89
Elbow.reconfigure :loose @15:02:06.93 Elbow.home.seek :clear @15:02:11.59 SE.reconfigure :default @15:02:11.63 SE.home.seek :down @15:02:12.28 SC.reconfigure :tight @15:02:12.32 SC.home.seek :initial @15:02:14.73 PS.stop @15:02:40.61
SC.configure :limp @15:02:40.66 Forearm.seek :retract @15:02:40.72 Elbow.seek :hammer @15:02:41.73 Forearm.reconfigure :tight @15:02:41.77 Forearm.seek retracted @15:02:42.02 CC.reconfigure :default @15:02:42.04 PC.reconfigure :default @15:02:42.13
CC.home.seek :open @15:02:42.15 PC.home.seek :open @15:02:45.70
Phase [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] @15:02:45.71 Notice.email "At 15:02:45 began in tube 3: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2/mission/DA_WCR_11723.rb:14\n{1 => 33,\n 2 => 7,\n 3 => 26,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@15:02:45, 20% flow, 14.2C, 31% humidity, 14.5psia, 13.749V, 0.405A, 0.393A avg, 5.57W",Subject: "Started DA_WCR_11723 phase initialPurge; da [1000, 1000] #da and wsr are run simultanelously" @15:02:45.73
Starting initialPurge @15:02:45.76 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @15:02:45.82 Hand.open @15:02:46.59 Elbow.reconfigure :tight @15:02:46.63 Elbow.seek FlushPuck::Garage @15:02:48.60 Elbow.reconfigure :loose @15:02:48.69 Forearm.reconfigure :loose @15:02:48.73 Forearm.seek :garage @15:02:49.71 Hand.close @15:02:50.49 Forearm.seek :retract @15:02:51.02 upsync @15:02:51.46
Elbow.reconfigure :tight @15:02:51.50 Elbow.seek CC @15:02:54.57 Elbow.reconfigure :loose @15:02:54.66 Forearm.reconfigure :tight @15:02:54.70 Forearm.seek CC @15:02:55.91 CC.seek :closed @15:03:00.42 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.37 @15:03:00.49 Hand.open @15:03:01.14 Forearm.reconfigure :loose @15:03:01.18 Forearm.seek :retract @15:03:02.14 Flushing the Collection Valve::Manifold @15:03:02.26 CSV.dial CSR @15:03:03.59 Pulling 5ml of CSR.flush @15:03:03.60 CSR.select :flush @15:03:03.71 CS.reconfigure :default @15:03:03.75 CS.seek 5ml @15:03:17.56 delay 5 seconds @15:03:22.57 CSR.select :air @15:03:22.73 CS.reconfigure :fast @15:03:22.77 CS.seek 7.5ml @15:03:29.74 CSV.dial :waste @15:03:31.01 CS.reconfigure :default @15:03:31.05 CS.seek 0ml @15:03:51.09 Purging external Intake @15:03:51.20 CSV.dial :flush @15:03:51.68 CS.reconfigure :fast @15:03:51.73 CS.seek 10ml @15:04:16.12 delay 5 seconds @15:04:21.14 CSV.dial CBV @15:04:21.17
CBV.dial CSV @15:04:21.91 CTV.dial :intake @15:04:22.72 Intake.select :open @15:04:22.83 CS.seek 0ml @15:04:46.39 Intake.select :close @15:04:46.49 CTV.dial :air @15:04:47.29 Flushing upper manifold @15:04:47.40 CSV.dial :flush @15:04:48.14 CS.seek 5ml @15:05:00.83 delay 5 seconds @15:05:05.85 CSV.dial :air @15:05:06.47 CS.seek 7ml @15:05:12.05 CSV.dial CBV @15:05:12.08
CBV.dial CSV @15:05:13.09 CTV.dial :air @15:05:13.10 CS.seek 0ml @15:05:29.74 delay 7 seconds @15:05:36.77 CS.seek 3ml @15:05:44.68 CSV.dial :waste @15:05:45.12 CS.seek 0ml @15:05:52.87 CSV.dial :flush @15:05:53.31 CS.seek 3ml @15:06:01.30 delay 5 seconds @15:06:06.32 CSV.dial :air @15:06:06.81 CS.seek 5.001ml @15:06:12.33 CSV.dial :air @15:06:12.35 CS.seek 0ml @15:06:24.61 delay 7 seconds @15:06:31.64 CS.seek 3ml @15:06:39.61 CSV.dial :waste @15:06:40.34 CS.seek 0ml @15:06:47.96 Garaging FlushPuck in Collection Clamp @15:06:48.03 Elbow.reconfigure :tight @15:06:48.07 Elbow.seek CC @15:06:48.43 Elbow.reconfigure :loose @15:06:48.52 Forearm.seek CC @15:06:49.48 Hand.close @15:06:50.08 CC.seek :open @15:06:54.69 Forearm.seek :retract @15:06:55.67 Elbow.reconfigure :tight @15:06:55.71 Elbow.seek :garage @15:06:58.61 Elbow.reconfigure :loose @15:06:58.70 Forearm.reconfigure :tight @15:06:58.74 Forearm.seek :garage @15:06:59.70 Hand.open @15:07:00.82 Forearm.reconfigure :loose @15:07:00.86 Forearm.seek :retract @15:07:01.79 da sampling at most 1000ml, wcr at most 1000ml @15:07:01.83 Loading sh1 puck from tube 3 into Collection Clamp @15:07:01.84 DAfans.email "Started da in tube 3 at 15:07:01UTC17-Jan-23\n{1 => 33,\n 2 => 7,\n 3 => 26,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@15:02:45, 20% flow, 14.2C, 31% humidity, 14.5psia, 13.749V, 0.405A, 0.393A avg, 5.57W",Subject: "da sampling at most 1000ml, wcr at most 1000ml" @15:07:02.09
SC.reconfigure :tight @15:07:02.13 SC.seek 3 @15:07:05.73 SC.configure :limp @15:07:05.94 Elbow.reconfigure :tight @15:07:05.99 Elbow.seek 3 @15:07:07.13 upsync @15:07:07.95
Forearm.reconfigure :tight @15:07:07.99 Forearm.seek 3 + 450 counts @15:07:08.99 SE.reconfigure :pick @15:07:09.05 SE.seek :beamBreak @15:07:10.57 SE.configure :default @15:07:10.72 Elbow.seek 3 @15:07:10.90 Forearm.seek 3 + 300 counts @15:07:11.17 Forearm.reconfigure :loose @15:07:11.21 Hand.close @15:07:11.84 SE.seek :down @15:07:12.04
Forearm.seek :retract @15:07:12.94 SE.stop @15:07:13.00
Elbow.seek CC @15:07:14.63 Elbow.reconfigure :loose @15:07:14.72 Forearm.reconfigure :tight @15:07:14.76 Forearm.seek CC @15:07:16.00 CC.seek :closed @15:07:20.50 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.28 @15:07:20.51 Hand.open @15:07:21.14 Forearm.reconfigure :loose @15:07:21.18 Forearm.seek :retract @15:07:22.14 collectSample 1000ml w/bubble point of 24psi @15:07:22.15 Priming 7ml @15:07:22.16 Intake.select :close @15:07:22.17 Exhaust.select :close @15:07:22.18 SS.hold @15:07:22.38 Calibrating pressure and temperature sensors @15:07:22.41 CTV.dial :air @15:07:22.42 SSV.dial :spit,avoiding: :exhaust @15:07:22.44 SS.reconfigure :fast @15:07:22.48 SS.seek 5ml @15:07:25.27 SSV.dial CBV @15:07:25.29
CBV.dial SSV @15:07:26.61 delay 3.8 seconds @15:07:30.53 SSV.dial :spit @15:07:31.85 SS.seek 0ml @15:07:34.56 SSV.dial CBV @15:07:35.85 CTV.dial :intake @15:07:36.63 SSV.dial CBV @15:07:36.65
CBV.dial SSV @15:07:36.67 Allowing 40s for 7.0ml of remaining 7.0ml @15:07:36.68 Intake.select :open @15:07:38.83 Intake @15.0psia, vacuum from 9.5 to 12.5psi @15:07:38.85 SS.reconfigure :inhaling 2.20ml/s @15:07:38.89 SS.seek 7ml @15:07:45.67 Stroke completed -- waiting for pressure to equalize ... @15:07:45.68 SS.reconfigure :exhale @15:07:45.72 SS.hold @15:07:46.59 Intake.select :close @15:07:46.73 SSV.dial :exhaust @15:07:47.47 Exhaust.select :open @15:07:47.58 SS.seek 0ml @15:07:51.61 Exhaust.select :close @15:07:51.71 Sampling 1000.0ml @15:07:51.72 Intake.select :close @15:07:51.73 Exhaust.select :close @15:07:51.74 SS.reconfigure :default @15:07:51.78 SS.hold @15:07:51.96 CTV.dial :intake @15:07:51.97 SSV.dial CBV @15:07:51.99
CBV.dial SSV @15:07:52.75 Allowing 130s for 25.0ml of remaining 1000.0ml @15:07:52.76 Intake.select :open @15:07:54.91 Intake @14.4psia, vacuum from 8.9 to 11.9psi @15:07:54.93 SS.reconfigure :inhaling 2.20ml/s @15:07:54.97 SS.seek 25ml @15:08:05.21 Vacuum too high -- waiting up to 40 seconds @15:08:05.22 SS.reconfigure :exhale @15:08:05.26 SS.hold @15:08:05.96 Intake @9.5psia, vacuum from 4.0 to 7.0psi @15:08:05.98 SS.reconfigure :inhaling 1.98ml/s @15:08:06.02 SS.seek 25ml @15:08:10.15 Vacuum too high -- waiting up to 40 seconds @15:08:10.16 SS.reconfigure :exhale @15:08:10.21 SS.hold @15:08:15.57 Intake @11.3psia, vacuum from 5.8 to 8.8psi @15:08:15.58 SS.reconfigure :inhaling 1.79ml/s @15:08:15.63 SS.seek 25ml @15:08:18.89 Stroke completed -- waiting for pressure to equalize ... @15:08:18.90 SS.reconfigure :exhale @15:08:18.94 SS.hold @15:08:19.80 Intake.select :close @15:08:19.93 SSV.dial :exhaust @15:08:20.68 Exhaust.select :open @15:08:20.79 SS.seek 0ml @15:08:29.50 Exhaust.select :close @15:08:29.60 SSV.dial CBV @15:08:29.62
CBV.dial SSV @15:08:30.38 Allowing 130s for 25.0ml of remaining 975.0ml @15:08:30.39 Intake.select :open @15:08:32.54 Intake @13.7psia, vacuum from 8.2 to 11.2psi @15:08:32.55 SS.reconfigure :inhaling 1.79ml/s @15:08:32.59 SS.seek 25ml @15:08:45.30 Vacuum too high -- waiting up to 40 seconds @15:08:45.31 SS.reconfigure :exhale @15:08:45.36 SS.hold @15:08:45.95 Intake @9.5psia, vacuum from 4.0 to 7.0psi @15:08:45.96 SS.reconfigure :inhaling 1.61ml/s @15:08:46.01 SS.seek 25ml @15:08:51.72 Stroke completed -- waiting for pressure to equalize ... @15:08:51.73 SS.reconfigure :exhale @15:08:51.77 SS.hold @15:08:57.30 Intake.select :close @15:08:57.44 SSV.dial :exhaust @15:08:58.19 Exhaust.select :open @15:08:58.29 SS.seek 0ml @15:09:07.12 Exhaust.select :close @15:09:07.23 SSV.dial CBV @15:09:07.28
CBV.dial SSV @15:09:07.98 Allowing 130s for 25.0ml of remaining 950.0ml @15:09:07.99 Intake.select :open @15:09:10.15 Intake @13.7psia, vacuum from 8.2 to 11.2psi @15:09:10.17 SS.reconfigure :inhaling 1.61ml/s @15:09:10.21 SS.seek 25ml @15:09:24.49 Vacuum too high -- waiting up to 40 seconds @15:09:24.50 SS.reconfigure :exhale @15:09:24.54 SS.hold @15:09:25.07 Intake @9.9psia, vacuum from 4.4 to 7.4psi @15:09:25.08 SS.reconfigure :inhaling 1.45ml/s @15:09:25.12 SS.seek 25ml @15:09:30.54 Stroke completed -- waiting for pressure to equalize ... @15:09:30.55 SS.reconfigure :exhale @15:09:30.59 SS.hold @15:09:37.02 Intake.select :close @15:09:37.16 SSV.dial :exhaust @15:09:37.90 Exhaust.select :open @15:09:38.01 SS.seek 0ml @15:09:46.83 Exhaust.select :close @15:09:46.93 SSV.dial CBV @15:09:46.95
CBV.dial SSV @15:09:47.70 Allowing 130s for 25.0ml of remaining 925.0ml @15:09:47.71 Intake.select :open @15:09:49.86 Intake @13.9psia, vacuum from 8.4 to 11.4psi @15:09:49.88 SS.reconfigure :inhaling 1.45ml/s @15:09:49.92 SS.seek 25ml @15:10:06.83 Vacuum too high -- waiting up to 40 seconds @15:10:06.84 SS.reconfigure :exhale @15:10:06.88 SS.hold @15:10:07.38 Intake @10.3psia, vacuum from 4.8 to 7.8psi @15:10:07.40 SS.reconfigure :inhaling 1.30ml/s @15:10:07.44 SS.seek 25ml @15:10:11.67 Stroke completed -- waiting for pressure to equalize ... @15:10:11.68 SS.reconfigure :exhale @15:10:11.72 SS.hold @15:10:18.15 Intake.select :close @15:10:18.29 SSV.dial :exhaust @15:10:19.03 Exhaust.select :open @15:10:19.14 SS.seek 0ml @15:10:27.97 Exhaust.select :close @15:10:28.07 SSV.dial CBV @15:10:28.09
CBV.dial SSV @15:10:28.84 Allowing 130s for 25.0ml of remaining 900.0ml @15:10:28.85 Intake.select :open @15:10:31.00 Intake @13.9psia, vacuum from 8.4 to 11.4psi @15:10:31.02 SS.reconfigure :inhaling 1.30ml/s @15:10:31.06 SS.seek 25ml @15:10:49.02 Vacuum too high -- waiting up to 40 seconds @15:10:49.03 SS.reconfigure :exhale @15:10:49.07 SS.hold @15:10:49.53 Intake @10.7psia, vacuum from 5.2 to 8.2psi @15:10:49.54 SS.reconfigure :inhaling 1.17ml/s @15:10:49.58 SS.seek 25ml @15:10:54.64 Stroke completed -- waiting for pressure to equalize ... SS.reconfigure :exhale @15:10:54.68 SS.hold @15:11:01.58 Intake.select :close @15:11:01.72 SSV.dial :exhaust @15:11:02.47 Exhaust.select :open @15:11:02.57 SS.seek 0ml @15:11:11.34 Exhaust.select :close @15:11:11.44 SSV.dial CBV @15:11:11.47
CBV.dial SSV @15:11:12.22 Allowing 130s for 25.0ml of remaining 875.0ml @15:11:12.23 Intake.select :open @15:11:14.38 Intake @14.1psia, vacuum from 8.6 to 11.6psi @15:11:14.39 SS.reconfigure :inhaling 1.17ml/s @15:11:14.44 SS.seek 25ml @15:11:37.02 Vacuum too high -- waiting up to 40 seconds @15:11:37.03 SS.reconfigure :exhale @15:11:37.07 SS.hold @15:11:37.72 Intake @11.1psia, vacuum from 5.6 to 8.6psi @15:11:37.74 SS.reconfigure :inhaling 1.06ml/s @15:11:37.78 SS.seek 25ml @15:11:40.60 Stroke completed -- waiting for pressure to equalize ... @15:11:40.61 SS.reconfigure :exhale @15:11:40.65 SS.hold @15:11:47.08 Intake.select :close @15:11:47.22 SSV.dial :exhaust @15:11:47.96 Exhaust.select :open @15:11:48.07 SS.seek 0ml @15:11:56.87 Exhaust.select :close @15:11:56.97 SSV.dial CBV @15:11:56.99
CBV.dial SSV @15:11:57.75 Allowing 130s for 25.0ml of remaining 850.0ml @15:11:57.76 Intake.select :open @15:11:59.92 Intake @14.2psia, vacuum from 8.7 to 11.7psi @15:11:59.93 SS.reconfigure :inhaling 1.06ml/s @15:11:59.97 SS.seek 25ml @15:12:26.18 Vacuum too high -- waiting up to 40 seconds @15:12:26.19 SS.reconfigure :exhale @15:12:26.23 SS.hold @15:12:27.76 Intake @11.5psia, vacuum from 6.0 to 9.0psi @15:12:27.77 SS.reconfigure :inhaling 0.95ml/s @15:12:27.81 SS.seek 25ml @15:12:28.15 Stroke completed -- waiting for pressure to equalize ... @15:12:28.16 SS.reconfigure :exhale @15:12:28.20 SS.hold @15:12:34.70 Intake.select :close @15:12:34.84 SSV.dial :exhaust @15:12:35.59 Exhaust.select :open @15:12:35.69 SS.seek 0ml @15:12:44.48 Exhaust.select :close @15:12:44.58 SSV.dial CBV @15:12:44.60
CBV.dial SSV @15:12:45.36 Allowing 130s for 25.0ml of remaining 825.0ml @15:12:45.37 Intake.select :open @15:12:47.52 Intake @14.3psia, vacuum from 8.8 to 11.8psi @15:12:47.53 SS.reconfigure :inhaling 0.95ml/s @15:12:47.57 SS.seek 25ml @15:13:16.66 Stroke completed -- waiting for pressure to equalize ... @15:13:16.67 SS.reconfigure :exhale @15:13:16.71 SS.hold @15:13:26.37 Intake.select :close @15:13:26.51 SSV.dial :exhaust @15:13:27.27 Exhaust.select :open @15:13:27.37 SS.seek 0ml @15:13:36.41 Exhaust.select :close @15:13:36.52 SSV.dial CBV @15:13:36.54
CBV.dial SSV @15:13:37.29 Allowing 130s for 25.0ml of remaining 800.0ml @15:13:37.30 Intake.select :open @15:13:39.45 Intake @14.3psia, vacuum from 8.8 to 11.8psi @15:13:39.47 SS.reconfigure :inhaling 0.95ml/s @15:13:39.51 SS.seek 25ml @15:14:05.61 Vacuum too high -- waiting up to 40 seconds @15:14:05.62 SS.reconfigure :exhale @15:14:05.66 SS.hold @15:14:09.68 Intake @12.2psia, vacuum from 6.7 to 9.7psi @15:14:09.70 SS.reconfigure :inhaling 0.87ml/s @15:14:09.74 SS.seek 25ml @15:14:12.79 Stroke completed -- waiting for pressure to equalize ... @15:14:12.80 SS.reconfigure :exhale @15:14:12.84 SS.hold @15:14:19.24 Intake.select :close @15:14:19.38 SSV.dial :exhaust @15:14:20.13 Exhaust.select :open @15:14:20.24 SS.seek 0ml @15:14:29.01 Exhaust.select :close @15:14:29.11 SSV.dial CBV @15:14:29.13
CBV.dial SSV @15:14:29.90 Allowing 130s for 25.0ml of remaining 775.0ml @15:14:29.91 Intake.select :open @15:14:32.06 Intake @14.3psia, vacuum from 8.8 to 11.8psi @15:14:32.08 SS.reconfigure :inhaling 0.87ml/s @15:14:32.12 SS.seek 25ml @15:14:59.33 Vacuum too high -- waiting up to 40 seconds @15:14:59.34 SS.reconfigure :exhale @15:14:59.38 SS.hold @15:15:02.91 Intake @12.5psia, vacuum from 7.0 to 10.0psi @15:15:02.93 SS.reconfigure :inhaling 0.78ml/s @15:15:02.97 SS.seek 25ml @15:15:08.40 Stroke completed -- waiting for pressure to equalize ... @15:15:08.41 SS.reconfigure :exhale @15:15:08.45 SS.hold @15:15:15.93 Intake.select :close @15:15:16.06 SSV.dial :exhaust @15:15:16.82 Exhaust.select :open @15:15:16.93 SS.seek 0ml @15:15:25.94 Exhaust.select :close @15:15:26.05 SSV.dial CBV @15:15:26.08
CBV.dial SSV @15:15:26.82 Allowing 130s for 25.0ml of remaining 750.0ml @15:15:26.83 Intake.select :open @15:15:28.98 Intake @14.4psia, vacuum from 8.9 to 11.9psi @15:15:29.00 SS.reconfigure :inhaling 0.78ml/s @15:15:29.04 SS.seek 25ml @15:16:03.47 Stroke completed -- waiting for pressure to equalize ... @15:16:03.48 SS.reconfigure :exhale @15:16:03.52 SS.hold @15:16:16.07 Intake.select :close @15:16:16.21 SSV.dial :exhaust @15:16:16.96 Exhaust.select :open @15:16:17.07 SS.seek 0ml @15:16:25.91 Exhaust.select :close @15:16:26.01 SSV.dial CBV @15:16:26.04
CBV.dial SSV @15:16:26.80 Allowing 130s for 25.0ml of remaining 725.0ml @15:16:26.81 Intake.select :open @15:16:28.97 Intake @14.3psia, vacuum from 8.8 to 11.8psi @15:16:28.98 SS.reconfigure :inhaling 0.78ml/s @15:16:29.03 SS.seek 25ml @15:16:53.57 Vacuum too high -- waiting up to 40 seconds @15:16:53.58 SS.reconfigure :exhale @15:16:53.63 SS.hold @15:16:59.24 Intake @13.1psia, vacuum from 7.6 to 10.6psi @15:16:59.26 SS.reconfigure :inhaling 0.70ml/s @15:16:59.30 SS.seek 25ml @15:17:11.11 Stroke completed -- waiting for pressure to equalize ... @15:17:11.12 SS.reconfigure :exhale @15:17:11.16 SS.hold @15:17:22.20 Intake.select :close @15:17:22.33 SSV.dial :exhaust @15:17:23.10 Exhaust.select :open @15:17:23.21 SS.seek 0ml @15:17:32.00 Exhaust.select :close @15:17:32.11 SSV.dial CBV @15:17:32.14
CBV.dial SSV @15:17:32.88 Allowing 130s for 25.0ml of remaining 700.0ml @15:17:32.89 Intake.select :open @15:17:35.04 Intake @14.4psia, vacuum from 8.9 to 11.9psi @15:17:35.06 SS.reconfigure :inhaling 0.70ml/s @15:17:35.10 SS.seek 25ml @15:18:03.17 Vacuum too high -- waiting up to 40 seconds @15:18:03.18 SS.reconfigure :exhale @15:18:03.22 SS.hold @15:18:08.83 Intake @13.0psia, vacuum from 7.5 to 10.5psi @15:18:08.84 SS.reconfigure :inhaling 0.63ml/s @15:18:08.88 SS.seek 25ml @15:18:20.41 Stroke completed -- waiting for pressure to equalize ... @15:18:20.42 SS.reconfigure :exhale @15:18:20.46 SS.hold @15:18:31.47 Intake.select :close @15:18:31.60 SSV.dial :exhaust @15:18:32.36 Exhaust.select :open @15:18:32.47 SS.seek 0ml @15:18:41.30 Exhaust.select :close @15:18:41.40 SSV.dial CBV @15:18:41.42
CBV.dial SSV @15:18:42.19 Allowing 130s for 25.0ml of remaining 675.0ml @15:18:42.20 Intake.select :open @15:18:44.35 Intake @14.4psia, vacuum from 8.9 to 11.9psi @15:18:44.37 SS.reconfigure :inhaling 0.63ml/s @15:18:44.41 SS.seek 25ml @15:19:11.42 Vacuum too high -- waiting up to 40 seconds @15:19:11.43 SS.reconfigure :exhale @15:19:11.47 SS.hold @15:19:16.30 Intake @12.7psia, vacuum from 7.2 to 10.2psi @15:19:16.31 SS.reconfigure :inhaling 0.57ml/s @15:19:16.36 SS.seek 25ml @15:19:33.59 Stroke completed -- waiting for pressure to equalize ... @15:19:33.60 SS.reconfigure :exhale @15:19:33.64 SS.hold @15:19:46.19 Intake.select :close @15:19:46.32 SSV.dial :exhaust @15:19:47.08 Exhaust.select :open @15:19:47.19 SS.seek 0ml @15:19:56.22 Exhaust.select :close @15:19:56.32 SSV.dial CBV @15:19:56.34
CBV.dial SSV @15:19:57.11 Allowing 130s for 25.0ml of remaining 650.0ml @15:19:57.12 Intake.select :open @15:19:59.27 Intake @14.3psia, vacuum from 8.8 to 11.8psi @15:19:59.29 SS.reconfigure :inhaling 0.57ml/s @15:19:59.33 SS.seek 25ml @15:20:34.98 Vacuum too high -- waiting up to 40 seconds @15:20:34.99 SS.reconfigure :exhale @15:20:35.03 SS.hold @15:20:41.14 Intake @13.0psia, vacuum from 7.5 to 10.5psi @15:20:41.15 SS.reconfigure :inhaling 0.51ml/s @15:20:41.20 SS.seek 25ml @15:20:52.75 Stroke completed -- waiting for pressure to equalize ... @15:20:52.76 SS.reconfigure :exhale @15:20:52.80 SS.hold @15:21:05.43 Intake.select :close @15:21:05.57 SSV.dial :exhaust @15:21:06.33 Exhaust.select :open @15:21:06.44 SS.seek 0ml @15:21:15.51 Exhaust.select :close @15:21:15.62 SSV.dial CBV @15:21:15.64
CBV.dial SSV @15:21:16.41 Allowing 130s for 25.0ml of remaining 625.0ml @15:21:16.42 Intake.select :open @15:21:18.57 Intake @14.2psia, vacuum from 8.7 to 11.7psi @15:21:18.59 SS.reconfigure :inhaling 0.51ml/s @15:21:18.63 SS.seek 25ml @15:22:03.13 Vacuum too high -- waiting up to 40 seconds @15:22:03.14 SS.reconfigure :exhale @15:22:03.18 SS.hold @15:22:10.52 Intake @13.0psia, vacuum from 7.5 to 10.5psi @15:22:10.54 SS.reconfigure :inhaling 0.47ml/s @15:22:10.58 SS.seek 25ml @15:22:17.54 Stroke completed -- waiting for pressure to equalize ... @15:22:17.55 SS.reconfigure :exhale @15:22:17.59 SS.hold @15:22:28.61 Intake.select :close @15:22:28.75 SSV.dial :exhaust @15:22:29.50 Exhaust.select :open @15:22:29.61 SS.seek 0ml @15:22:38.42 Exhaust.select :close @15:22:38.52 SSV.dial CBV @15:22:38.55
CBV.dial SSV @15:22:39.31 Allowing 130s for 25.0ml of remaining 600.0ml @15:22:39.32 Intake.select :open @15:22:41.47 Intake @14.3psia, vacuum from 8.8 to 11.8psi @15:22:41.49 SS.reconfigure :inhaling 0.47ml/s @15:22:41.53 SS.seek 25ml @15:23:26.54 Vacuum too high -- waiting up to 40 seconds SS.reconfigure :exhale @15:23:26.58 SS.hold @15:23:34.04 Intake @13.1psia, vacuum from 7.6 to 10.6psi @15:23:34.05 SS.reconfigure :inhaling 0.43ml/s @15:23:34.10 SS.seek 25ml @15:23:46.61 Stroke completed -- waiting for pressure to equalize ... @15:23:46.62 SS.reconfigure :exhale @15:23:46.66 SS.hold @15:23:59.43 Intake.select :close @15:23:59.56 SSV.dial :exhaust @15:24:00.33 Exhaust.select :open @15:24:00.43 SS.seek 0ml @15:24:09.48 Exhaust.select :close @15:24:09.58 SSV.dial CBV @15:24:09.60
CBV.dial SSV @15:24:10.38 Allowing 130s for 25.0ml of remaining 575.0ml @15:24:10.39 Intake.select :open @15:24:12.54 Intake @14.3psia, vacuum from 8.8 to 11.8psi @15:24:12.55 SS.reconfigure :inhaling 0.43ml/s @15:24:12.60 SS.seek 25ml @15:25:12.63 Vacuum too high -- waiting up to 40 seconds @15:25:12.64 SS.reconfigure :exhale @15:25:12.68 SS.hold @15:25:22.31 Intake @13.5psia, vacuum from 8.0 to 11.0psi @15:25:22.33 SS.reconfigure :inhaling 0.38ml/s @15:25:22.37 SS.seek 25ml @15:25:24.48 Stroke completed -- waiting for pressure to equalize ... @15:25:24.49 SS.reconfigure :exhale @15:25:24.53 SS.hold @15:25:35.63 Intake.select :close @15:25:35.77 SSV.dial :exhaust @15:25:36.53 Exhaust.select :open @15:25:36.63 SS.seek 0ml @15:25:45.40 Exhaust.select :close @15:25:45.50 SSV.dial CBV @15:25:45.53
CBV.dial SSV @15:25:46.29 Allowing 130s for 25.0ml of remaining 550.0ml @15:25:46.30 Intake.select :open @15:25:48.46 Intake @14.4psia, vacuum from 8.9 to 11.9psi @15:25:48.48 SS.reconfigure :inhaling 0.38ml/s @15:25:48.52 SS.seek 25ml @15:26:57.97 Vacuum too high -- waiting up to 40 seconds @15:26:57.98 SS.reconfigure :exhale @15:26:58.02 SS.hold @15:27:07.68 Intake @13.6psia, vacuum from 8.1 to 11.1psi @15:27:07.69 SS.reconfigure :inhaling 0.34ml/s @15:27:07.74 SS.seek 25ml @15:27:09.59 Stroke completed -- waiting for pressure to equalize ... @15:27:09.60 SS.reconfigure :exhale @15:27:09.64 SS.hold @15:27:21.15 Intake.select :close @15:27:21.29 SSV.dial :exhaust @15:27:22.06 Exhaust.select :open @15:27:22.16 SS.seek 0ml @15:27:31.01 Exhaust.select :close @15:27:31.11 SSV.dial CBV @15:27:31.13
CBV.dial SSV @15:27:31.90 Allowing 130s for 25.0ml of remaining 525.0ml @15:27:31.91 Intake.select :open @15:27:34.07 Intake @14.4psia, vacuum from 8.9 to 11.9psi @15:27:34.08 SS.reconfigure :inhaling 0.34ml/s @15:27:34.12 SS.seek 25ml @15:28:56.96 Stroke completed -- waiting for pressure to equalize ... @15:28:56.97 SS.reconfigure :exhale @15:28:57.01 SS.hold @15:29:17.39 Intake.select :close @15:29:17.53 SSV.dial :exhaust @15:29:18.28 Exhaust.select :open @15:29:18.39 SS.seek 0ml @15:29:27.19 Exhaust.select :close @15:29:27.30 SSV.dial CBV @15:29:27.33
CBV.dial SSV @15:29:28.06 Allowing 130s for 25.0ml of remaining 500.0ml @15:29:28.07 Intake.select :open @15:29:30.22 Intake @14.4psia, vacuum from 8.9 to 11.9psi @15:29:30.23 SS.reconfigure :inhaling 0.34ml/s @15:29:30.28 SS.seek 25ml @15:30:53.71 Stroke completed -- waiting for pressure to equalize ... @15:30:53.72 SS.reconfigure :exhale @15:30:53.76 SS.hold @15:31:20.17 Intake.select :close @15:31:20.30 SSV.dial :exhaust @15:31:21.06 Exhaust.select :open @15:31:21.16 SS.seek 0ml @15:31:29.99 Exhaust.select :close @15:31:30.09 SSV.dial CBV @15:31:30.11
CBV.dial SSV @15:31:30.87 Allowing 130s for 25.0ml of remaining 475.0ml @15:31:30.88 Intake.select :open @15:31:33.03 Intake @14.6psia, vacuum from 9.1 to 12.1psi @15:31:33.05 SS.reconfigure :inhaling 0.34ml/s @15:31:33.09 SS.seek 25ml @15:32:40.34 Vacuum too high -- waiting up to 40 seconds @15:32:40.35 SS.reconfigure :exhale @15:32:40.39 SS.hold @15:32:54.73 Intake @14.0psia, vacuum from 8.5 to 11.5psi @15:32:54.75 SS.reconfigure :inhaling 0.31ml/s @15:32:54.79 SS.seek 25ml @15:33:12.67 Stroke completed -- waiting for pressure to equalize ... @15:33:12.68 SS.reconfigure :exhale @15:33:12.72 SS.hold @15:33:33.61 Intake.select :close @15:33:33.75 SSV.dial :exhaust @15:33:34.49 Exhaust.select :open @15:33:34.59 SS.seek 0ml @15:33:43.41 Exhaust.select :close @15:33:43.52 SSV.dial CBV @15:33:43.53
CBV.dial SSV @15:33:44.28 Allowing 130s for 25.0ml of remaining 450.0ml @15:33:44.29 Intake.select :open @15:33:46.45 Intake @14.6psia, vacuum from 9.1 to 12.1psi @15:33:46.46 SS.reconfigure :inhaling 0.31ml/s @15:33:46.51 SS.seek 25ml @15:35:09.52 Vacuum too high -- waiting up to 40 seconds @15:35:09.53 SS.reconfigure :exhale @15:35:09.57 SS.hold @15:35:23.84 Intake @14.1psia, vacuum from 8.6 to 11.6psi @15:35:23.85 SS.reconfigure :inhaling 0.28ml/s @15:35:23.90 SS.seek 25ml @15:35:36.79 Stroke completed -- waiting for pressure to equalize ... @15:35:36.80 SS.reconfigure :exhale @15:35:36.84 SS.hold @15:35:54.30 SS.reconfigure :default @15:35:54.34 SS.hold @15:35:54.45 Intake.select :close @15:35:54.59 ShallowSampler::Clogged in MAIN -- Filtering too Slowly after 575.0ml @15:35:54.60 SSV.dial :exhaust @15:35:55.34 Exhaust.select :open @15:35:55.45 SS.reconfigure :exhale @15:35:55.49 SS.seek 0ml @15:36:04.53 Exhaust.select :close @15:36:04.63 Sampled 575.0ml @15:36:04.64 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @15:36:04.65 Intake.select :close @15:36:04.71 CSV.dial :waste @15:36:04.77 CS.seek 0ml @15:36:04.85 CSV.dial :air @15:36:04.91 SSV.dial CBV,avoiding: :exhaust @15:36:04.92
CBV.dial SSV @15:36:05.61 CS.seek 10ml @15:36:06.80
CTV.dial :air @15:36:07.59 delay 15 seconds @15:36:22.61 SSV.dial :spit,avoiding: :exhaust @15:36:23.95 CTV.dial CSV,avoiding: :intake @15:36:23.98
SS.seek 0ml @15:36:24.09 SSV.dial CBV,avoiding: :exhaust @15:36:30.24 CBV.dial between SSV and waste2 @15:36:30.62 CTV.dial CSV,avoiding: :air @15:36:30.64
CSV.dial CTV @15:36:31.48 CS.reconfigure :pressurize @15:36:31.62 CS.seek 0ml @15:36:50.31 BubblePoint: Intake @38.6psia, Outlet @14.4psia @15:36:50.32 CBV.dial SSV @15:36:50.64 SS.reconfigure :default @15:36:50.68 SS.seek 10ml @15:36:56.23 delay 15 seconds @15:37:11.35 Evacuated: Intake @16.6psia, Outlet @9.1psia @15:37:11.36 SSV.dial :spit,avoiding: :exhaust @15:37:12.68 SS.reconfigure :exhale @15:37:12.72 SS.seek 0ml @15:37:17.95 SS.reconfigure :fast @15:37:17.99 SS.seek 5ml @15:37:20.81 SSV.dial CBV,avoiding: :exhaust @15:37:20.84
CBV.dial SSV @15:37:22.16 CTV.dial :air,avoiding: :intake @15:37:23.52 CSV.dial CTV @15:37:23.55
SS.seek 10ml @15:37:24.55 CSV.dial :air @15:37:25.01 CS.reconfigure :fast @15:37:25.06 CS.seek 0ml @15:37:26.05
SSV.dial :spit,avoiding: :exhaust @15:37:27.38 SS.reconfigure :exhale @15:37:27.42 SS.seek 0ml @15:37:32.41 SS.reconfigure :fast @15:37:32.45 SS.seek 5ml @15:37:32.55 CS.stop @15:37:35.28 SSV.dial CBV,avoiding: :exhaust @15:37:35.31
CBV.dial SSV @15:37:37.63 SSV.dial :spit,avoiding: :exhaust @15:37:38.96 SS.reconfigure :default @15:37:39.00 SS.seek 0ml @15:37:42.23 SS.stop @15:37:42.27 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @15:37:42.28 Intake.select :close @15:37:42.35 CSV.dial :waste @15:37:42.46 SSV.dial CBV,avoiding: :exhaust @15:37:42.48
CBV.dial SSV @15:37:43.13 CS.seek 0ml @15:37:43.19 CSV.dial :air @15:37:43.80
CTV.dial :air @15:37:43.81 delay 15 seconds @15:37:43.92 CS.seek 10ml @15:37:58.83
SSV.dial :spit,avoiding: :exhaust @15:38:00.15 CTV.dial CSV,avoiding: :intake @15:38:00.17
SS.reconfigure :exhale @15:38:00.27 SS.seek 0ml @15:38:00.66 SSV.dial CBV,avoiding: :exhaust @15:38:08.27 CBV.dial between SSV and waste2 @15:38:08.65 CTV.dial CSV,avoiding: :air @15:38:08.67
CSV.dial CTV @15:38:09.11 CS.reconfigure :pressurize @15:38:09.15 CS.seek 0ml @15:38:30.46 BubblePoint: Intake @37.7psia, Outlet @13.4psia @15:38:30.47 CBV.dial SSV @15:38:30.79 SS.reconfigure :default @15:38:30.84 SS.seek 10ml @15:38:36.40 delay 15 seconds @15:38:51.52 Evacuated: Intake @18.0psia, Outlet @11.0psia @15:38:51.54 SSV.dial :spit,avoiding: :exhaust @15:38:52.86 SS.reconfigure :exhale @15:38:52.90 SS.seek 0ml @15:38:58.18 SS.reconfigure :fast @15:38:58.22 SS.seek 5ml @15:39:01.08 SSV.dial CBV,avoiding: :exhaust @15:39:01.10
CBV.dial SSV @15:39:02.44 CTV.dial :air,avoiding: :intake @15:39:03.78 CSV.dial CTV @15:39:03.81
SS.seek 10ml @15:39:04.80 CSV.dial :air @15:39:05.25 CS.reconfigure :fast @15:39:05.29 CS.seek 0ml @15:39:06.28
SSV.dial :spit,avoiding: :exhaust @15:39:07.61 SS.reconfigure :exhale @15:39:07.65 SS.seek 0ml @15:39:10.43 CS.stop @15:39:12.65
SS.reconfigure :fast @15:39:12.69 SS.seek 5ml @15:39:15.49 SSV.dial CBV,avoiding: :exhaust @15:39:15.51
CBV.dial SSV @15:39:17.83 SSV.dial :spit,avoiding: :exhaust @15:39:19.14 SS.reconfigure :default @15:39:19.18 SS.seek 0ml @15:39:22.56 SS.stop @15:39:22.59 Lysing: Adding 2.0ml AqMeOH @15:39:22.60 CBV.dial between CSV and waste1,avoiding: :DBV @15:39:24.09 CTV.dial CSV @15:39:25.41 CSV.dial CSR @15:39:26.20 Pulling 1ml of CSR.AqMeOH @15:39:26.21 CSR.select :AqMeOH @15:39:26.70 CS.reconfigure :default @15:39:26.80 CS.seek 1ml @15:39:30.24 delay 5 seconds @15:39:35.26 CSR.select :air @15:39:35.42 CS.seek 6.001ml @15:39:49.02 delay 2 seconds @15:39:51.04 CSV.dial :waste2 @15:39:51.47 CS.reconfigure :fast @15:39:51.51 CS.seek 0ml @15:40:05.86 CSV.dial CSR @15:40:06.37 Pulling 1.999ml of CSR.AqMeOH @15:40:06.38 CSR.select :AqMeOH @15:40:06.49 CS.reconfigure :default @15:40:06.53 CS.seek 2ml @15:40:12.68 delay 5 seconds @15:40:17.70 CSR.select :air @15:40:17.85 CS.seek 5ml @15:40:26.49 delay 2 seconds @15:40:28.50 CSV.dial CTV @15:40:28.95 CS.seek 1ml @15:40:40.10 Before lysing Collection Puck Heater at 14.8C/38.0psia @15:40:40.12 Heating Puck.from 3, :sh1 in Collection Clamp to 70C for 10:00 @15:40:40.14 CH.seek 70C,8:00 @15:42:43.67 CH.hold 10:00,70C @15:42:43.85 Begun lysing Collection Puck Heater at 68C/46.0psia @15:52:43.87 Ended lysing Collection Puck Heater at 70C/44.9psia @15:52:43.88 CS.seek 0ml @15:52:47.49 CTV.dial between spare and air,avoiding: :intake @15:52:48.53 CSV.dial :air @15:52:48.98 CS.seek 5ml @15:53:02.57 CSV.dial CTV @15:53:03.01 delay 2 seconds @15:53:05.03 CSV.dial :air @15:53:05.48 delay 2 seconds @15:53:07.50 CSV.dial CTV @15:53:07.95 delay 2 seconds @15:53:09.96 CSV.dial :air @15:53:10.42 delay 2 seconds @15:53:12.44 CS.seek 0ml @15:53:26.06 Puck @72.8psia after final lysis pressurization @15:53:26.07 Recovering Lysate into Collection syringe @15:53:26.08 CSV.dial CBV @15:53:27.09 CBV.dial CSV @15:53:27.46 CS.seek 5ml @15:53:41.47 delay 5 seconds @15:53:46.49 CTV.dial :air @15:53:47.01 CS.seek 7ml @15:53:52.88 delay 5 seconds @15:53:57.90 CSV.dial :air2 @15:53:58.84 CS.seek 10ml @15:54:07.42 CSV.dial between air and CTV,avoiding: CBV @15:54:08.58 Lysing Complete @15:54:08.64 Storing sh1 puck in Collection Clamp to tube 2 @15:54:08.80 SC.reconfigure :tight @15:54:08.84 SC.seek 2 @15:54:12.37 SC.configure :limp @15:54:12.57 Elbow.reconfigure :tight @15:54:12.61 Elbow.seek 2 @15:54:14.37 Forearm.reconfigure :tight @15:54:14.41 Forearm.seek 2 + 450 counts @15:54:15.41 SE.reconfigure :pick @15:54:15.46 SE.seek :beamBreak @15:54:18.26 SE.configure :default @15:54:18.40 Elbow.reconfigure :limp @15:54:18.44 Forearm.reconfigure :loose @15:54:18.48 Forearm.seek 2 @15:54:18.79 Elbow.hold @15:54:18.84 Elbow.reconfigure :tight @15:54:18.94 SE.seek 6.12 Pucks below Top Plate @15:54:19.37 Forearm.force -20 @15:54:19.40 Forearm.seek 2 + 255 counts @15:54:19.68 Forearm.reconfigure :tight @15:54:19.72 Forearm.seek 2 + 450 counts @15:54:19.93 SE.seek 7.12 Pucks below Top Plate @15:54:20.45 Forearm.reconfigure :loose @15:54:20.49 Forearm.seek :retract @15:54:21.40 Elbow.seek CC @15:54:23.09 Elbow.reconfigure :loose @15:54:23.18 Forearm.seek CC @15:54:24.18 Hand.close @15:54:24.79 CC.seek :open @15:54:29.50 Forearm.seek :retract @15:54:30.45 Elbow.reconfigure :tight @15:54:30.49 Elbow.seek 2 @15:54:32.27 Elbow.reconfigure :limp @15:54:32.31 Forearm.seek 2 @15:54:33.24 Elbow.hold @15:54:33.30 Elbow.reconfigure :tight @15:54:33.34 Hand.open @15:54:34.02 Forearm.force -20 @15:54:34.05 Forearm.seek 2 + 296 counts @15:54:34.34 Forearm.reconfigure :tight @15:54:34.39 Forearm.seek 2 + 450 counts @15:54:34.63 SE.seek :down @15:54:35.75
Forearm.reconfigure :loose @15:54:35.79 Forearm.seek :retract @15:54:36.84 SE.stop @15:54:36.88
Tranferring DA @15:54:36.89 Beginning samplePassC @15:54:36.91 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @15:54:37.09 Elbow.seek FlushPuck::Garage @15:54:38.97 Elbow.reconfigure :loose @15:54:39.06 Forearm.seek :garage @15:54:40.05 Hand.close @15:54:40.77 Forearm.seek :retract @15:54:41.77 Elbow.reconfigure :tight @15:54:41.81 Elbow.seek CC @15:54:44.81 Elbow.reconfigure :loose @15:54:44.91 Forearm.reconfigure :tight @15:54:44.95 Forearm.seek CC @15:54:46.15 CC.seek :closed @15:54:50.79 Collection Clamp onSpring/offSpring for 11/26 samples, on/off torque 2.26 @15:54:50.86 Hand.open @15:54:51.47 Forearm.reconfigure :loose @15:54:51.51 Forearm.seek :retract @15:54:52.46 PSV.dial PSR @15:54:53.84 Pulling 1.5ml of PSR.flush @15:54:53.85 PSR.select :flush @15:54:53.96 PS.seek 1.5ml @15:55:10.05 delay 5 seconds @15:55:15.07 PSV.dial :waste1 @15:55:15.54 PS.seek 0ml @15:55:31.73 PSV.dial PSR @15:55:32.19 PSR.select :air @15:55:32.30 PS.seek 2.5ml @15:55:58.62 PRV.dial CBV,avoiding: :ab2 @15:55:58.63
CBV.dial PRV @15:56:00.33 PSV.dial :mixloop @15:56:01.38 CTV.dial :air,avoiding: :intake @15:56:01.39 PTV.dial :PRVmixing @15:56:02.73 PS.seek 0ml @15:56:29.11 PSV.dial :air @15:56:29.57 PS.seek 2.5ml @15:56:55.89 PSV.dial :mixloop @15:56:56.36 PS.seek 0ml @15:57:22.73 PSV.dial :air @15:57:23.19 PS.seek 2.5ml @15:57:49.54 PSV.dial :mixloop @15:57:50.01 PS.seek 0ml @15:58:16.33 Garaging FlushPuck in Collection Clamp @15:58:16.40 Elbow.reconfigure :tight @15:58:16.44 Elbow.seek CC @15:58:16.79 Elbow.reconfigure :loose @15:58:16.88 Forearm.seek CC @15:58:17.89 Hand.close @15:58:18.54 CC.seek :open @15:58:23.17 Forearm.seek :retract @15:58:24.15 Elbow.reconfigure :tight @15:58:24.19 Elbow.seek :garage @15:58:27.17 Elbow.reconfigure :loose @15:58:27.25 Forearm.reconfigure :tight @15:58:27.30 Forearm.seek :garage @15:58:28.23 Hand.open @15:58:28.84 Forearm.reconfigure :loose @15:58:28.88 Forearm.seek :retract @15:58:29.85 PSV.dial :mixloop @15:58:29.86 PTV.dial :PRVmixing @15:58:29.87 PRV.dial CBV @15:58:29.89
CBV.dial PRV @15:58:29.91 CTV.dial :air,avoiding: :intake @15:58:29.93 Loading lyfil puck from tube 3 into Collection Clamp @15:58:30.15 SC.reconfigure :tight @15:58:30.20 SC.seek 3 @15:58:33.71 SC.configure :limp @15:58:33.90 Elbow.reconfigure :tight @15:58:33.94 Elbow.seek 3 @15:58:35.92 Forearm.reconfigure :tight @15:58:35.97 Forearm.seek 3 + 450 counts @15:58:37.01 SE.reconfigure :pick @15:58:37.07 SE.seek :beamBreak @15:58:38.59 SE.configure :default @15:58:38.74 Elbow.seek 3 @15:58:38.91 Forearm.seek 3 + 300 counts @15:58:39.20 Forearm.reconfigure :loose @15:58:39.24 Hand.close @15:58:39.92 SE.seek :down @15:58:40.10
Forearm.seek :retract @15:58:41.07 SE.stop @15:58:41.14
Elbow.seek CC @15:58:42.76 Elbow.reconfigure :loose @15:58:42.85 Forearm.reconfigure :tight @15:58:42.89 Forearm.seek CC @15:58:44.62 CC.seek :closed @15:58:49.13 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.10 @15:58:49.14 Hand.open @15:58:49.84 Forearm.reconfigure :loose @15:58:49.88 Forearm.seek :retract @15:58:50.83 CTV.dial CSV,avoiding: :intake @15:58:52.19 CSV.dial CTV @15:58:52.53 PS.seek 2.5ml @15:59:18.98 CS.seek 4.5ml @15:59:33.82 delay 8 seconds @15:59:41.84 PSV.dial :air @15:59:42.29 delay 2 seconds @15:59:44.31 PSV.dial :waste2 @15:59:45.06 PS.seek 0ml @16:00:11.36 CBV.dial between PRV and waste1 @16:00:11.68 CSV.dial :air @16:00:12.14 CS.seek 10ml @16:00:27.06 CSV.dial CTV @16:00:27.51 CS.seek 0ml @16:00:53.91 CBV.dial :waste1 @16:00:54.21 delay 5 seconds @16:00:59.23 CTV.dial :air,avoiding: :intake @16:01:00.64 Storing lyfil puck in Collection Clamp to tube 2 @16:01:00.79 SC.reconfigure :tight @16:01:00.84 SC.seek 2 @16:01:04.36 SC.configure :limp @16:01:04.55 Elbow.reconfigure :tight @16:01:04.59 Elbow.seek 2 @16:01:06.36 Forearm.reconfigure :tight @16:01:06.40 Forearm.seek 2 + 450 counts @16:01:07.42 SE.reconfigure :pick @16:01:07.47 SE.seek :beamBreak @16:01:10.12 SE.configure :default @16:01:10.26 Elbow.reconfigure :limp @16:01:10.30 Forearm.reconfigure :loose @16:01:10.34 Forearm.seek 2 @16:01:10.56 Elbow.hold @16:01:10.62 Elbow.reconfigure :tight @16:01:10.71 SE.seek 7.13 Pucks below Top Plate @16:01:11.13 Forearm.force -20 @16:01:11.17 Forearm.seek 2 + 308 counts @16:01:11.44 Forearm.reconfigure :tight @16:01:11.49 Forearm.seek 2 + 450 counts @16:01:11.69 SE.seek 8.13 Pucks below Top Plate @16:01:12.22 Forearm.reconfigure :loose @16:01:12.26 Forearm.seek :retract @16:01:13.16 Elbow.seek CC @16:01:14.87 Elbow.reconfigure :loose @16:01:14.95 Forearm.seek CC @16:01:15.92 Hand.close @16:01:16.53 CC.seek :open @16:01:21.25 Forearm.seek :retract @16:01:22.22 Elbow.reconfigure :tight @16:01:22.26 Elbow.seek 2 @16:01:24.04 Elbow.reconfigure :limp @16:01:24.08 Forearm.seek 2 @16:01:25.06 Elbow.hold @16:01:25.12 Elbow.reconfigure :tight @16:01:25.17 Hand.open @16:01:25.89 Forearm.force -20 @16:01:25.92 Forearm.seek 2 + 302 counts @16:01:26.70 Forearm.reconfigure :tight @16:01:26.75 Forearm.seek 2 + 450 counts @16:01:27.02 SE.seek :down @16:01:28.15
Forearm.reconfigure :loose @16:01:28.19 Forearm.seek :retract @16:01:29.20 SE.stop @16:01:29.23
PSV.dial :air @16:01:29.99 PS.seek 2.5ml @16:01:56.35 delay 1 second @16:01:57.37 PSV.dial :mixloop @16:01:57.81 delay 1 second @16:01:58.82 PSV.dial :air @16:01:59.27 delay 1 second @16:02:00.29 PSV.dial :mixloop @16:02:00.87 delay 1 second @16:02:01.88 PSV.dial :air @16:02:02.34 delay 1 second @16:02:03.36 PS.seek 0ml @16:02:29.85 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @16:02:30.03 Elbow.seek FlushPuck::Garage @16:02:31.91 Elbow.reconfigure :loose @16:02:32.00 Forearm.seek :garage @16:02:33.01 Hand.close @16:02:33.77 Forearm.seek :retract @16:02:34.77 Elbow.reconfigure :tight @16:02:34.81 Elbow.seek PC @16:02:36.05 Elbow.reconfigure :loose @16:02:36.16 Forearm.reconfigure :tight @16:02:36.20 Forearm.seek PC @16:02:37.41 PC.seek :closed @16:02:42.01 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.53 @16:02:42.07 Hand.open @16:02:42.71 Forearm.reconfigure :loose @16:02:42.75 Forearm.seek :retract @16:02:43.68 PRV.dial :air @16:02:44.13 PSV.dial :mixloop @16:02:44.66 PS.seek 0.3ml @16:02:48.57 PSV.dial PSR @16:02:49.66 Pulling 0.4ml of PSR.air @16:02:49.67 PSR.select :air @16:02:49.69 PS.seek 0.7ml @16:02:54.54 delay 3 seconds @16:02:57.55 PSR.select :air @16:02:57.58 PS.seek 2.5ml @16:03:16.82 delay 3 seconds @16:03:19.84 PSV.dial :mixloop @16:03:20.88 PTV.dial :mixing @16:03:21.63 PBV.dial :waste2 @16:03:22.58 PS.seek 0ml @16:03:48.91 Garaging FlushPuck in Processing Clamp @16:03:48.98 Elbow.reconfigure :tight @16:03:49.02 Elbow.seek PC @16:03:49.13 Elbow.reconfigure :loose @16:03:49.21 Forearm.seek PC @16:03:50.14 Hand.close @16:03:50.78 PC.seek :open @16:03:55.49 Forearm.seek :retract @16:03:56.42 Elbow.reconfigure :tight @16:03:56.46 Elbow.seek :garage @16:03:57.82 Elbow.reconfigure :loose @16:03:57.91 Forearm.reconfigure :tight @16:03:57.95 Forearm.seek :garage @16:03:58.95 Hand.open @16:03:59.59 Forearm.reconfigure :loose @16:03:59.63 Forearm.seek :retract @16:04:00.61 Loading da puck from tube 3 into Processing Clamp @16:04:00.82 SC.reconfigure :tight @16:04:00.87 SC.seek 3 @16:04:04.37 SC.configure :limp @16:04:05.02 Elbow.reconfigure :tight @16:04:05.07 Elbow.seek 3 @16:04:06.95 Forearm.reconfigure :tight @16:04:07.00 Forearm.seek 3 + 450 counts @16:04:08.07 SE.reconfigure :pick @16:04:08.13 SE.seek :beamBreak @16:04:09.68 SE.configure :default @16:04:09.82 Elbow.seek 3 @16:04:09.99 Forearm.seek 3 + 300 counts @16:04:10.12 Forearm.reconfigure :loose @16:04:10.16 Hand.close @16:04:10.79 SE.seek :down @16:04:10.98
Forearm.seek :retract @16:04:11.99 SE.stop @16:04:12.05
Elbow.seek PC @16:04:14.63 Elbow.reconfigure :loose @16:04:14.72 Forearm.reconfigure :tight @16:04:14.76 Forearm.seek PC @16:04:16.00 PC.seek :closed @16:04:20.63 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.59 @16:04:20.64 Hand.open @16:04:21.34 Forearm.reconfigure :loose @16:04:21.38 Forearm.seek :retract @16:04:22.33 Heating Puck.from 3, :da in Processing Clamp to 28C for 2:00:00 @16:04:22.35 PH.seek 28C,5:00 @16:05:02.19 PH.hold 2:00:00,28C @16:05:02.23 delay 1:30 @16:06:32.27 PSR.select :air @16:06:32.29 PSV.dial PSR @16:06:33.40 Pulling 0.5ml of PSR.wash @16:06:33.41 PSR.select :wash @16:06:33.53 PS.seek 0.5ml @16:06:39.51 delay 5 seconds @16:06:44.52 PSR.select :air @16:06:44.64 PS.seek 2ml @16:07:00.85 PTV.dial :puck @16:07:01.58 PSV.dial PTV @16:07:02.47 PBV.dial :waste1 @16:07:03.88 PS.seek 1.5ml @16:07:09.78 delay 3 seconds @16:07:12.80 PBV.dial between PSV and waste1 @16:07:13.17 PS.seek 0ml @16:07:29.35 delay 5:00 @16:12:29.37 recoverPSRreagent08 @16:12:29.38 PTV.dial :puck @16:12:29.39 PBV.dial :waste1 @16:12:29.75 delay 30 seconds @16:12:59.77 PBV.dial between PSV and waste1 @16:13:00.14 PSR.select :air @16:13:00.15 PSV.dial PSR @16:13:00.91 PS.reconfigure :fast @16:13:00.95 PS.seek 2.5ml @16:13:24.68 PSV.dial PTV @16:13:25.43 delay 5 seconds @16:13:30.45 PSV.dial PSR @16:13:31.20 delay 5 seconds @16:13:36.22 PSV.dial PTV @16:13:36.98 delay 5 seconds @16:13:42.00 PSV.dial :air @16:13:42.88 PS.seek 0ml @16:14:06.03 PTV.dial :PRVmixing,via: PRV @16:14:07.35 PSV.dial :mixloop @16:14:07.79 PRV.dial :ab1 @16:14:08.53 PS.reconfigure :slow @16:14:08.58 PS.seek 0.15ml @16:14:12.15 delay 2 seconds @16:14:14.65 CBV.dial PRV @16:14:14.67
PRV.dial CBV @16:14:15.24 PS.seek 0.35ml @16:14:19.84 delay 2 seconds @16:14:21.85 CBV.dial between PRV and PSV @16:14:22.18 PRV.dial :air @16:14:22.63 PS.seek 0.9ml @16:14:34.40 PRV.dial :waste2 @16:14:35.14 PS.seek 0.5ml @16:14:43.78 addPMLReagent08 @16:14:43.79 PSV.dial :air @16:14:44.25 PS.reconfigure :default @16:14:44.29 PS.seek 2ml @16:15:00.40 PSV.dial :PML @16:15:00.85 PTV.dial :mixing @16:15:01.62 PBV.dial :waste1 @16:15:02.05 PS.seek 1.85ml @16:15:04.46 PBV.dial between PSV and waste1 @16:15:04.85 PS.seek 0ml @16:15:24.49 delay 2 seconds @16:15:26.51 Beginning samplePassC @16:15:26.52 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @16:15:26.69 Elbow.reconfigure :tight @16:15:26.73 Elbow.seek FlushPuck::Garage @16:15:28.08 Elbow.reconfigure :loose @16:15:28.16 Forearm.seek :garage @16:15:29.18 Hand.close @16:15:29.89 Forearm.seek :retract @16:15:30.84 Elbow.reconfigure :tight @16:15:30.88 Elbow.seek CC @16:15:33.81 Elbow.reconfigure :loose @16:15:33.90 Forearm.reconfigure :tight @16:15:33.94 Forearm.seek CC @16:15:35.22 CC.seek :closed @16:15:39.72 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.34 @16:15:39.79 Hand.open @16:15:40.46 Forearm.reconfigure :loose @16:15:40.50 Forearm.seek :retract @16:15:41.50 PSV.dial PSR @16:15:42.61 Pulling 1.5ml of PSR.flush @16:15:42.62 PSR.select :flush @16:15:42.73 PS.seek 1.5ml @16:15:58.90 delay 5 seconds @16:16:03.92 PSV.dial :waste1 @16:16:04.37 PS.seek 0ml @16:16:20.46 PSV.dial PSR @16:16:20.91 PSR.select :air @16:16:21.02 PS.seek 2.5ml @16:16:47.40 PRV.dial CBV,avoiding: :ab2 @16:16:47.42
CBV.dial PRV @16:16:49.07 PSV.dial :mixloop @16:16:50.21 CTV.dial :air,avoiding: :intake @16:16:50.22 PTV.dial :PRVmixing @16:16:50.98 PS.seek 0ml @16:17:17.36 PSV.dial :air @16:17:17.84 PS.seek 2.5ml @16:17:44.21 PSV.dial :mixloop @16:17:44.68 PS.seek 0ml @16:18:10.99 PSV.dial :air @16:18:11.45 PS.seek 2.5ml @16:18:37.86 PSV.dial :mixloop @16:18:38.32 PS.seek 0ml @16:19:04.65 Garaging FlushPuck in Collection Clamp @16:19:04.72 Elbow.reconfigure :tight @16:19:04.76 Elbow.seek CC @16:19:05.10 Elbow.reconfigure :loose @16:19:05.19 Forearm.seek CC @16:19:06.14 Hand.close @16:19:06.77 CC.seek :open @16:19:11.85 Forearm.seek :retract @16:19:12.80 Elbow.reconfigure :tight @16:19:12.84 Elbow.seek :garage @16:19:15.81 Elbow.reconfigure :loose @16:19:15.90 Forearm.reconfigure :tight @16:19:15.94 Forearm.seek :garage @16:19:16.93 Hand.open @16:19:17.58 Forearm.reconfigure :loose @16:19:17.62 Forearm.seek :retract @16:19:18.59 delayUntil "16:23:26.50" @16:19:18.61 Whole Cell Archive sampling 1000ml @16:19:18.62 Loading wcr puck from tube 3 into Collection Clamp @16:19:19.07 Elbow.reconfigure :tight @16:19:19.12 Elbow.seek 3 @16:19:21.09 Forearm.reconfigure :tight @16:19:21.13 Forearm.seek 3 + 450 counts @16:19:22.18 SE.reconfigure :pick @16:19:22.23 SE.seek :beamBreak @16:19:23.81 SE.configure :default @16:19:23.96 Elbow.seek 3 @16:19:24.14 Forearm.seek 3 + 300 counts @16:19:24.34 Forearm.reconfigure :loose @16:19:24.39 Hand.close @16:19:25.03 SE.seek :down @16:19:25.23 Forearm.seek :retract @16:19:26.15 Elbow.seek CC @16:19:26.36 SE.stop @16:19:27.73 Elbow.reconfigure :loose @16:19:27.82 Forearm.reconfigure :tight @16:19:27.87 Forearm.seek CC @16:19:29.06 CC.seek :closed @16:19:33.58 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.20 @16:19:33.59 Hand.open @16:19:34.20 Forearm.reconfigure :loose @16:19:34.25 Forearm.seek :retract @16:19:35.19 collectSample 1000ml w/bubble point of 35psi @16:19:35.21 Priming 7ml Intake.select :close @16:19:35.22 Exhaust.select :close @16:19:35.23 SS.hold @16:19:35.43 Calibrating pressure sensors @16:19:35.44 CTV.dial :air @16:19:35.45 SSV.dial :spit,avoiding: :exhaust @16:19:35.47 SS.reconfigure :fast @16:19:35.51 SS.seek 5ml @16:19:38.35 SSV.dial CBV @16:19:38.37 CBV.dial SSV @16:19:39.75 delay 3.8 seconds @16:19:43.66 SSV.dial :spit @16:19:44.97 SS.seek 0ml @16:19:47.56 SSV.dial CBV @16:19:48.89 CTV.dial :intake @16:19:49.66 SSV.dial CBV @16:19:49.68 CBV.dial SSV @16:19:49.70 Allowing 40s for 7.0ml of remaining 7.0ml @16:19:49.71 Intake.select :open @16:19:51.86 Intake @15.0psia, vacuum from 9.5 to 12.5psi @16:19:51.88 SS.reconfigure :inhaling 2.20ml/s @16:19:51.93 SS.seek 7ml @16:19:58.18 Stroke completed -- waiting for pressure to equalize ... @16:19:58.19 SS.reconfigure :exhale @16:19:58.23 SS.hold @16:20:06.22 Intake.select :close @16:20:06.36 SSV.dial :exhaust @16:20:07.11 Exhaust.select :open @16:20:07.22 SS.seek 0ml @16:20:11.15 Exhaust.select :close @16:20:11.26 Sampling 1000.0ml @16:20:11.27 Intake.select :close @16:20:11.28 Exhaust.select :close @16:20:11.29 SS.reconfigure :default @16:20:11.33 SS.hold @16:20:11.53 CTV.dial :intake @16:20:11.55 SSV.dial CBV @16:20:11.59 CBV.dial SSV @16:20:12.33 Allowing 130s for 25.0ml of remaining 1000.0ml @16:20:12.34 Intake.select :open @16:20:14.49 Intake @15.1psia, vacuum from 9.6 to 12.6psi @16:20:14.51 SS.reconfigure :inhaling 2.20ml/s @16:20:14.55 SS.seek 25ml @16:20:21.47 Vacuum too high -- waiting up to 40 seconds @16:20:21.48 SS.reconfigure :exhale @16:20:21.53 SS.hold @16:20:22.21 Intake @11.9psia, vacuum from 6.4 to 9.4psi @16:20:22.23 SS.reconfigure :inhaling 1.98ml/s @16:20:22.27 SS.seek 25ml @16:20:24.97 Vacuum too high -- waiting up to 40 seconds @16:20:24.98 SS.reconfigure :exhale @16:20:25.03 SS.hold @16:20:30.12 Intake @12.7psia, vacuum from 7.2 to 10.2psi @16:20:30.14 SS.reconfigure :inhaling 1.79ml/s @16:20:30.18 SS.seek 25ml @16:20:37.22 Vacuum too high -- waiting up to 40 seconds @16:20:37.23 SS.reconfigure :exhale @16:20:37.27 SS.hold @16:20:41.64 Intake @12.1psia, vacuum from 6.6 to 9.6psi @16:20:41.66 SS.reconfigure :inhaling 1.61ml/s @16:20:41.70 SS.seek 25ml @16:20:43.18 Stroke completed -- waiting for pressure to equalize ... @16:20:43.19 SS.reconfigure :exhale @16:20:43.23 SS.hold @16:20:44.88 Intake.select :close @16:20:45.02 SSV.dial :exhaust @16:20:45.79 Exhaust.select :open @16:20:45.90 SS.seek 0ml @16:20:54.71 Exhaust.select :close @16:20:54.82 SSV.dial CBV @16:20:54.84 CBV.dial SSV @16:20:55.62 Allowing 130s for 25.0ml of remaining 975.0ml @16:20:55.63 Intake.select :open @16:20:57.79 Intake @14.5psia, vacuum from 9.0 to 12.0psi @16:20:57.81 SS.reconfigure :inhaling 1.61ml/s @16:20:57.85 SS.seek 25ml @16:21:10.07 Vacuum too high -- waiting up to 40 seconds @16:21:10.08 SS.reconfigure :exhale @16:21:10.12 SS.hold @16:21:10.67 Intake @11.1psia, vacuum from 5.6 to 8.6psi @16:21:10.69 SS.reconfigure :inhaling 1.45ml/s @16:21:10.73 SS.seek 25ml @16:21:15.94 Vacuum too high -- waiting up to 40 seconds @16:21:15.95 SS.reconfigure :exhale @16:21:15.99 SS.hold @16:21:22.07 Intake @12.6psia, vacuum from 7.1 to 10.1psi @16:21:22.09 SS.reconfigure :inhaling 1.30ml/s @16:21:22.13 SS.seek 25ml @16:21:25.38 Stroke completed -- waiting for pressure to equalize ... @16:21:25.39 SS.reconfigure :exhale @16:21:25.44 SS.hold @16:21:27.40 Intake.select :close @16:21:27.54 SSV.dial :exhaust @16:21:28.32 Exhaust.select :open @16:21:28.43 SS.seek 0ml @16:21:37.21 Exhaust.select :close @16:21:37.32 SSV.dial CBV @16:21:37.34 CBV.dial SSV @16:21:38.12 Allowing 130s for 25.0ml of remaining 950.0ml @16:21:38.13 Intake.select :open @16:21:40.29 Intake @14.6psia, vacuum from 9.1 to 12.1psi @16:21:40.31 SS.reconfigure :inhaling 1.30ml/s @16:21:40.35 SS.seek 25ml @16:22:00.69 Vacuum too high -- waiting up to 40 seconds @16:22:00.70 SS.reconfigure :exhale @16:22:00.74 SS.hold @16:22:01.22 Intake @11.3psia, vacuum from 5.8 to 8.8psi @16:22:01.24 SS.reconfigure :inhaling 1.17ml/s @16:22:01.28 SS.seek 25ml @16:22:03.65 Stroke completed -- waiting for pressure to equalize ... @16:22:03.66 SS.reconfigure :exhale @16:22:03.70 SS.hold @16:22:10.11 Intake.select :close @16:22:10.25 SSV.dial :exhaust @16:22:11.02 Exhaust.select :open @16:22:11.13 SS.seek 0ml @16:22:19.93 Exhaust.select :close @16:22:20.04 SSV.dial CBV @16:22:20.06 CBV.dial SSV @16:22:20.84 Allowing 130s for 25.0ml of remaining 925.0ml @16:22:20.85 Intake.select :open @16:22:23.01 Intake @14.6psia, vacuum from 9.1 to 12.1psi @16:22:23.02 SS.reconfigure :inhaling 1.17ml/s @16:22:23.07 SS.seek 25ml @16:22:47.15 Stroke completed -- waiting for pressure to equalize ... @16:22:47.16 SS.reconfigure :exhale @16:22:47.20 SS.hold @16:22:54.58 Intake.select :close @16:22:54.72 SSV.dial :exhaust @16:22:55.48 Exhaust.select :open @16:22:55.59 SS.seek 0ml @16:23:04.62 Exhaust.select :close @16:23:04.72 SSV.dial CBV @16:23:04.74 CBV.dial SSV @16:23:05.51 Allowing 130s for 25.0ml of remaining 900.0ml @16:23:05.52 Intake.select :open @16:23:07.68 Intake @14.5psia, vacuum from 9.0 to 12.0psi @16:23:07.69 SS.reconfigure :inhaling 1.17ml/s @16:23:07.74 SS.seek 25ml @16:23:26.51 recoverPRVreagent08 @16:23:26.52 PTV.dial :mixing @16:23:26.81 Vacuum too high -- waiting up to 40 seconds @16:23:26.82 SS.reconfigure :exhale @16:23:26.87 SS.hold @16:23:27.69 PBV.dial :waste1 @16:23:27.86 Intake @11.7psia, vacuum from 6.2 to 9.2psi @16:23:27.88 SS.reconfigure :inhaling 1.06ml/s @16:23:27.92 SS.seek 25ml @16:23:28.10 delay 30 seconds @16:23:33.77 Stroke completed -- waiting for pressure to equalize ... @16:23:33.78 SS.reconfigure :exhale @16:23:33.83 SS.hold @16:23:39.41 Intake.select :close @16:23:39.55 SSV.dial :exhaust @16:23:40.34 Exhaust.select :open @16:23:40.45 SS.seek 0ml @16:23:49.26 Exhaust.select :close @16:23:49.36 SSV.dial CBV @16:23:49.39 CBV.dial SSV @16:23:50.15 Allowing 130s for 25.0ml of remaining 875.0ml @16:23:50.16 Intake.select :open @16:23:52.32 Intake @14.6psia, vacuum from 9.1 to 12.1psi @16:23:52.33 SS.reconfigure :inhaling 1.06ml/s @16:23:52.38 SS.seek 25ml @16:23:58.11 PBV.dial between PSV and waste1 @16:23:58.49 PSV.dial :air @16:23:58.96 PS.reconfigure :fast @16:23:59.00 PS.seek 2.5ml @16:24:18.91 Stroke completed -- waiting for pressure to equalize ... @16:24:18.92 SS.reconfigure :exhale @16:24:18.97 SS.hold @16:24:22.86 PSV.dial :mixloop @16:24:23.31 delay 2 seconds @16:24:25.34 PSV.dial :air @16:24:25.81 delay 2 seconds @16:24:26.35 Intake.select :close @16:24:26.49 SSV.dial :exhaust @16:24:27.26 Exhaust.select :open @16:24:27.37 SS.seek 0ml @16:24:27.83 PSV.dial :mixloop @16:24:28.30 delay 2 seconds @16:24:30.31 PSV.dial :air @16:24:30.93 PS.seek 0ml @16:24:36.38 Exhaust.select :close @16:24:36.49 SSV.dial CBV @16:24:36.51 CBV.dial SSV @16:24:37.29 Allowing 130s for 25.0ml of remaining 850.0ml @16:24:37.30 Intake.select :open @16:24:39.46 Intake @14.5psia, vacuum from 9.0 to 12.0psi @16:24:39.48 SS.reconfigure :inhaling 1.06ml/s @16:24:39.52 SS.seek 25ml @16:24:54.39 washCycleDA #1 of 2 @16:24:54.40 PSR.select :air @16:24:54.42 PSV.dial PSR @16:24:55.80 Pulling 0.5ml of PSR.wash @16:24:55.81 PSR.select :wash @16:24:55.92 PS.reconfigure :default @16:24:55.97 PS.seek 0.5ml @16:25:00.09 Vacuum too high -- waiting up to 40 seconds @16:25:00.10 SS.reconfigure :exhale @16:25:00.15 SS.hold @16:25:01.79 Intake @12.0psia, vacuum from 6.5 to 9.5psi @16:25:01.81 SS.reconfigure :inhaling 0.95ml/s @16:25:01.85 SS.seek 25ml @16:25:01.96 delay 5 seconds @16:25:06.97 PSR.select :air @16:25:07.08 PS.seek 2ml @16:25:08.35 Stroke completed -- waiting for pressure to equalize ... @16:25:08.36 SS.reconfigure :exhale @16:25:08.40 SS.hold @16:25:15.41 Intake.select :close @16:25:15.56 SSV.dial :exhaust @16:25:16.32 Exhaust.select :open @16:25:16.43 SS.seek 0ml @16:25:23.26 PTV.dial :puck @16:25:24.01 PSV.dial PTV @16:25:24.78 PBV.dial :waste1 @16:25:25.21 PS.seek 1.5ml @16:25:25.33 Exhaust.select :close @16:25:25.44 SSV.dial CBV @16:25:25.46 CBV.dial SSV @16:25:26.24 Allowing 130s for 25.0ml of remaining 825.0ml @16:25:26.26 Intake.select :open @16:25:28.41 Intake @14.5psia, vacuum from 9.0 to 12.0psi @16:25:28.43 SS.reconfigure :inhaling 0.95ml/s @16:25:28.47 SS.seek 25ml @16:25:31.20 delay 3 seconds @16:25:34.22 PBV.dial between PSV and waste1 @16:25:34.60 PS.seek 0ml @16:25:50.92 delay 2:00 @16:25:52.62 Vacuum too high -- waiting up to 40 seconds @16:25:52.63 SS.reconfigure :exhale @16:25:52.68 SS.hold @16:25:54.33 Intake @12.1psia, vacuum from 6.6 to 9.6psi @16:25:54.35 SS.reconfigure :inhaling 0.87ml/s @16:25:54.39 SS.seek 25ml @16:26:00.16 Stroke completed -- waiting for pressure to equalize ... @16:26:00.17 SS.reconfigure :exhale @16:26:00.21 SS.hold @16:26:06.71 Intake.select :close @16:26:06.85 SSV.dial :exhaust @16:26:07.61 Exhaust.select :open @16:26:07.72 SS.seek 0ml @16:26:16.86 Exhaust.select :close @16:26:16.97 SSV.dial CBV @16:26:16.99 CBV.dial SSV @16:26:17.76 Allowing 130s for 25.0ml of remaining 800.0ml @16:26:17.77 Intake.select :open @16:26:19.92 Intake @14.4psia, vacuum from 8.9 to 11.9psi @16:26:19.94 SS.reconfigure :inhaling 0.87ml/s @16:26:19.98 SS.seek 25ml @16:26:50.06 Vacuum too high -- waiting up to 40 seconds @16:26:50.07 SS.reconfigure :exhale @16:26:50.11 SS.hold @16:26:53.11 Intake @12.5psia, vacuum from 7.0 to 10.0psi @16:26:53.12 SS.reconfigure :inhaling 0.78ml/s @16:26:53.17 SS.seek 25ml @16:26:54.16 Stroke completed -- waiting for pressure to equalize ... @16:26:54.17 SS.reconfigure :exhale @16:26:54.21 SS.hold @16:26:59.85 Intake.select :close @16:26:59.99 SSV.dial :exhaust @16:27:00.75 Exhaust.select :open @16:27:00.86 SS.seek 0ml @16:27:09.66 Exhaust.select :close @16:27:09.77 SSV.dial CBV @16:27:09.82 CBV.dial SSV @16:27:10.54 Allowing 130s for 25.0ml of remaining 775.0ml @16:27:11.04 Intake.select :open @16:27:13.19 Intake @14.5psia, vacuum from 9.0 to 12.0psi @16:27:13.21 SS.reconfigure :inhaling 0.78ml/s @16:27:13.25 SS.seek 25ml @16:27:48.25 Stroke completed -- waiting for pressure to equalize ... @16:27:48.26 SS.reconfigure :exhale @16:27:48.31 SS.hold @16:27:50.94 recoverPSRreagent08 @16:27:50.95 PTV.dial :puck @16:27:50.96 PBV.dial :waste1 @16:27:51.34 delay 30 seconds @16:27:56.77 Intake.select :close @16:27:56.91 SSV.dial :exhaust @16:27:57.67 Exhaust.select :open @16:27:57.78 SS.seek 0ml @16:28:06.67 Exhaust.select :close @16:28:06.78 SSV.dial CBV @16:28:06.80 CBV.dial SSV @16:28:07.58 Allowing 130s for 25.0ml of remaining 750.0ml @16:28:07.59 Intake.select :open @16:28:09.75 Intake @14.5psia, vacuum from 9.0 to 12.0psi @16:28:09.77 SS.reconfigure :inhaling 0.78ml/s @16:28:09.81 SS.seek 25ml @16:28:21.36 PBV.dial between PSV and waste1 @16:28:21.73 PSR.select :air @16:28:21.75 PSV.dial PSR @16:28:22.52 PS.reconfigure :fast @16:28:22.56 PS.seek 2.5ml @16:28:38.53 Vacuum too high -- waiting up to 40 seconds @16:28:38.54 SS.reconfigure :exhale @16:28:38.58 SS.hold @16:28:41.58 Intake @12.6psia, vacuum from 7.1 to 10.1psi @16:28:41.59 SS.reconfigure :inhaling 0.70ml/s @16:28:41.64 SS.seek 25ml @16:28:46.38 PSV.dial PTV @16:28:47.17 delay 5 seconds @16:28:48.36 Stroke completed -- waiting for pressure to equalize ... @16:28:48.37 SS.reconfigure :exhale @16:28:48.41 SS.hold @16:28:52.18 PSV.dial PSR @16:28:52.95 delay 5 seconds @16:28:55.77 Intake.select :close @16:28:55.92 SSV.dial :exhaust @16:28:56.67 Exhaust.select :open @16:28:56.78 SS.seek 0ml @16:28:57.96 PSV.dial PTV @16:28:58.73 delay 5 seconds @16:29:03.75 PSV.dial :air @16:29:04.52 PS.seek 0ml @16:29:05.62 Exhaust.select :close @16:29:05.73 SSV.dial CBV @16:29:05.75 CBV.dial SSV @16:29:06.53 Allowing 130s for 25.0ml of remaining 725.0ml @16:29:06.55 Intake.select :open @16:29:08.70 Intake @14.6psia, vacuum from 9.1 to 12.1psi @16:29:08.72 SS.reconfigure :inhaling 0.70ml/s @16:29:08.77 SS.seek 25ml @16:29:28.04 washCycleDA #2 of 2 @16:29:28.05 PSR.select :air @16:29:28.07 PSV.dial PSR @16:29:29.45 Pulling 0.5ml of PSR.wash @16:29:29.46 PSR.select :wash @16:29:29.57 PS.reconfigure :default @16:29:29.62 PS.seek 0.5ml @16:29:35.61 delay 5 seconds @16:29:40.62 PSR.select :air @16:29:40.74 PS.seek 2ml @16:29:46.83 Stroke completed -- waiting for pressure to equalize ... @16:29:46.84 SS.reconfigure :exhale @16:29:46.88 SS.hold @16:29:56.87 PTV.dial :puck @16:29:56.89 PSV.dial PTV @16:29:57.08 Intake.select :close @16:29:57.22 SSV.dial :exhaust @16:29:57.66 PBV.dial :waste1 @16:29:57.98 Exhaust.select :open @16:29:58.12 PS.seek 1.5ml @16:29:58.17 SS.seek 0ml @16:30:04.04 delay 3 seconds @16:30:07.06 PBV.dial between PSV and waste1 @16:30:07.23 Exhaust.select :close @16:30:07.34 SSV.dial CBV @16:30:07.36 CBV.dial SSV @16:30:07.44 PS.seek 0ml @16:30:08.13 Allowing 130s for 25.0ml of remaining 700.0ml @16:30:08.14 Intake.select :open @16:30:10.30 Intake @14.6psia, vacuum from 9.1 to 12.1psi @16:30:10.31 SS.reconfigure :inhaling 0.70ml/s @16:30:10.36 SS.seek 25ml @16:30:23.67 delay 2:00 @16:30:42.02 Vacuum too high -- waiting up to 40 seconds @16:30:42.03 SS.reconfigure :exhale @16:30:42.08 SS.hold @16:30:46.25 Intake @13.1psia, vacuum from 7.6 to 10.6psi @16:30:46.27 SS.reconfigure :inhaling 0.63ml/s @16:30:46.31 SS.seek 25ml @16:30:54.11 Stroke completed -- waiting for pressure to equalize ... @16:30:54.12 SS.reconfigure :exhale @16:30:54.16 SS.hold @16:31:02.04 Intake.select :close @16:31:02.18 SSV.dial :exhaust @16:31:02.95 Exhaust.select :open @16:31:03.06 SS.seek 0ml @16:31:11.93 Exhaust.select :close @16:31:12.04 SSV.dial CBV @16:31:12.09 CBV.dial SSV @16:31:12.83 Allowing 130s for 25.0ml of remaining 675.0ml @16:31:12.84 Intake.select :open @16:31:14.99 Intake @14.7psia, vacuum from 9.2 to 12.2psi @16:31:15.01 SS.reconfigure :inhaling 0.63ml/s @16:31:15.06 SS.seek 25ml @16:31:48.37 Vacuum too high -- waiting up to 40 seconds @16:31:48.39 SS.reconfigure :exhale @16:31:48.43 SS.hold @16:31:52.48 Intake @13.1psia, vacuum from 7.6 to 10.6psi @16:31:52.50 SS.reconfigure :inhaling 0.57ml/s @16:31:52.54 SS.seek 25ml @16:32:02.56 Stroke completed -- waiting for pressure to equalize ... @16:32:02.57 SS.reconfigure :exhale @16:32:02.61 SS.hold @16:32:10.35 Intake.select :close @16:32:10.49 SSV.dial :exhaust @16:32:11.25 Exhaust.select :open @16:32:11.36 SS.seek 0ml @16:32:20.56 Exhaust.select :close @16:32:20.67 SSV.dial CBV @16:32:20.71 CBV.dial SSV @16:32:21.45 Allowing 130s for 25.0ml of remaining 650.0ml @16:32:21.46 Intake.select :open @16:32:23.63 Intake @14.7psia, vacuum from 9.2 to 12.2psi @16:32:23.64 SS.reconfigure :inhaling 0.57ml/s @16:32:23.69 SS.seek 25ml @16:32:23.73 recoverPSRreagent08 @16:32:23.74 PTV.dial :puck @16:32:23.75 PBV.dial :waste1 @16:32:24.12 delay 30 seconds @16:32:54.14 PBV.dial between PSV and waste1 @16:32:54.51 PSR.select :air @16:32:54.52 PSV.dial PSR @16:32:55.29 PS.reconfigure :fast @16:32:55.34 PS.seek 2.5ml @16:33:09.18 Stroke completed -- waiting for pressure to equalize ... @16:33:09.19 SS.reconfigure :exhale @16:33:09.24 SS.hold @16:33:19.16 PSV.dial PTV @16:33:19.93 delay 5 seconds @16:33:21.80 Intake.select :close @16:33:21.94 SSV.dial :exhaust @16:33:22.70 Exhaust.select :open @16:33:22.81 SS.seek 0ml @16:33:24.95 PSV.dial PSR @16:33:25.72 delay 5 seconds @16:33:30.74 PSV.dial PTV @16:33:31.51 delay 5 seconds @16:33:31.90 Exhaust.select :close @16:33:32.01 SSV.dial CBV @16:33:32.03 CBV.dial SSV @16:33:32.79 Allowing 130s for 25.0ml of remaining 625.0ml @16:33:32.81 Intake.select :open @16:33:34.96 Intake @14.7psia, vacuum from 9.2 to 12.2psi @16:33:34.98 SS.reconfigure :inhaling 0.57ml/s @16:33:35.02 SS.seek 25ml @16:33:36.52 PSV.dial :air @16:33:37.42 PS.seek 0ml @16:34:00.75 getPMLReagent08 @16:34:00.76 PRV.dial :air @16:34:01.21 delay 5 seconds @16:34:06.23 PTV.dial :PRVmixing @16:34:07.55 delay 5 seconds @16:34:12.57 PRV.dial :ab2 @16:34:13.02 PTV.dial :PRVmixing @16:34:13.03 PSV.dial :mixloop @16:34:13.11 Vacuum too high -- waiting up to 40 seconds @16:34:13.13 SS.reconfigure :exhale @16:34:13.17 SS.hold @16:34:13.54 PS.reconfigure :default @16:34:13.59 PS.seek 0.3ml @16:34:17.56 delay 2 seconds @16:34:18.81 Intake @13.4psia, vacuum from 7.9 to 10.9psi @16:34:18.83 SS.reconfigure :inhaling 0.51ml/s @16:34:18.87 SS.seek 25ml @16:34:19.59 PRV.dial :air @16:34:20.11 PS.seek 0.55ml @16:34:23.51 addPMLReagent08 @16:34:23.52 PSV.dial :air @16:34:23.98 PS.seek 2ml @16:34:28.30 Stroke completed -- waiting for pressure to equalize ... @16:34:28.32 SS.reconfigure :exhale @16:34:28.36 SS.hold @16:34:36.94 Intake.select :close @16:34:37.08 SSV.dial :exhaust @16:34:37.82 Exhaust.select :open @16:34:37.93 SS.seek 0ml @16:34:40.06 PSV.dial :PML @16:34:40.53 PTV.dial :mixing @16:34:41.88 PBV.dial :waste1 @16:34:42.31 PS.seek 1.85ml @16:34:44.66 PBV.dial between PSV and waste1 @16:34:45.04 PS.seek 0ml @16:34:47.01 Exhaust.select :close @16:34:47.12 SSV.dial CBV @16:34:47.15 CBV.dial SSV @16:34:47.90 Allowing 130s for 25.0ml of remaining 600.0ml @16:34:47.91 Intake.select :open @16:34:50.07 Intake @14.6psia, vacuum from 9.1 to 12.1psi @16:34:50.09 SS.reconfigure :inhaling 0.51ml/s @16:34:50.14 SS.seek 25ml @16:35:04.85 delay 2 seconds @16:35:06.87 delay 8:00 @16:35:37.24 Vacuum too high -- waiting up to 40 seconds @16:35:37.25 SS.reconfigure :exhale @16:35:37.29 SS.hold @16:35:43.79 Intake @13.5psia, vacuum from 8.0 to 11.0psi @16:35:43.81 SS.reconfigure :inhaling 0.47ml/s @16:35:43.86 SS.seek 25ml @16:35:48.08 Stroke completed -- waiting for pressure to equalize ... @16:35:48.09 SS.reconfigure :exhale @16:35:48.13 SS.hold @16:35:56.58 Intake.select :close @16:35:56.73 SSV.dial :exhaust @16:35:57.49 Exhaust.select :open @16:35:57.60 SS.seek 0ml @16:36:06.46 Exhaust.select :close @16:36:06.57 SSV.dial CBV @16:36:06.60 CBV.dial SSV @16:36:07.35 Allowing 130s for 25.0ml of remaining 575.0ml @16:36:07.37 Intake.select :open @16:36:09.52 Intake @14.8psia, vacuum from 9.3 to 12.3psi @16:36:09.54 SS.reconfigure :inhaling 0.47ml/s @16:36:09.58 SS.seek 25ml @16:37:05.69 Stroke completed -- waiting for pressure to equalize ... @16:37:05.70 SS.reconfigure :exhale @16:37:05.75 SS.hold @16:37:20.15 Intake.select :close @16:37:20.30 SSV.dial :exhaust @16:37:21.05 Exhaust.select :open @16:37:21.16 SS.seek 0ml @16:37:30.26 Exhaust.select :close @16:37:30.36 SSV.dial CBV @16:37:30.39 CBV.dial SSV @16:37:31.15 Allowing 130s for 25.0ml of remaining 550.0ml @16:37:31.16 Intake.select :open @16:37:33.32 Intake @14.7psia, vacuum from 9.2 to 12.2psi @16:37:33.33 SS.reconfigure :inhaling 0.47ml/s @16:37:33.38 SS.seek 25ml @16:38:18.20 Vacuum too high -- waiting up to 40 seconds @16:38:18.21 SS.reconfigure :exhale @16:38:18.26 SS.hold @16:38:24.80 Intake @13.4psia, vacuum from 7.9 to 10.9psi @16:38:24.82 SS.reconfigure :inhaling 0.43ml/s @16:38:24.86 SS.seek 25ml @16:38:36.82 Stroke completed -- waiting for pressure to equalize ... @16:38:36.83 SS.reconfigure :exhale @16:38:36.87 SS.hold @16:38:47.91 Intake.select :close @16:38:48.05 SSV.dial :exhaust @16:38:48.80 Exhaust.select :open @16:38:48.91 SS.seek 0ml @16:38:57.92 Exhaust.select :close @16:38:58.03 SSV.dial CBV @16:38:58.05 CBV.dial SSV @16:38:58.82 Allowing 130s for 25.0ml of remaining 525.0ml @16:38:58.83 Intake.select :open @16:39:00.99 Intake @14.6psia, vacuum from 9.1 to 12.1psi @16:39:01.01 SS.reconfigure :inhaling 0.43ml/s @16:39:01.05 SS.seek 25ml @16:39:57.45 Vacuum too high -- waiting up to 40 seconds @16:39:57.46 SS.reconfigure :exhale @16:39:57.50 SS.hold @16:40:03.89 Intake @13.5psia, vacuum from 8.0 to 11.0psi @16:40:03.91 SS.reconfigure :inhaling 0.38ml/s @16:40:03.95 SS.seek 25ml @16:40:10.00 Stroke completed -- waiting for pressure to equalize ... @16:40:10.01 SS.reconfigure :exhale @16:40:10.05 SS.hold @16:40:21.25 Intake.select :close @16:40:21.39 SSV.dial :exhaust @16:40:22.14 Exhaust.select :open @16:40:22.26 SS.seek 0ml @16:40:31.30 Exhaust.select :close @16:40:31.41 SSV.dial CBV @16:40:31.43 CBV.dial SSV @16:40:32.21 Allowing 130s for 25.0ml of remaining 500.0ml @16:40:32.22 Intake.select :open @16:40:34.38 Intake @14.5psia, vacuum from 9.0 to 12.0psi @16:40:34.39 SS.reconfigure :inhaling 0.38ml/s @16:40:34.44 SS.seek 25ml @16:41:43.95 Stroke completed -- waiting for pressure to equalize ... @16:41:43.96 SS.reconfigure :exhale @16:41:44.01 SS.hold @16:42:02.24 Intake.select :close @16:42:02.38 SSV.dial :exhaust @16:42:03.14 Exhaust.select :open @16:42:03.25 SS.seek 0ml @16:42:12.08 Exhaust.select :close @16:42:12.18 SSV.dial CBV @16:42:12.24 CBV.dial SSV @16:42:12.95 Allowing 130s for 25.0ml of remaining 475.0ml @16:42:12.97 Intake.select :open @16:42:15.12 Intake @14.7psia, vacuum from 9.2 to 12.2psi @16:42:15.14 SS.reconfigure :inhaling 0.38ml/s @16:42:15.18 SS.seek 25ml @16:43:06.89 recoverPRVreagent08 @16:43:06.90 PTV.dial :mixing @16:43:06.91 PBV.dial :waste1 @16:43:07.29 delay 30 seconds @16:43:15.32 Vacuum too high -- waiting up to 40 seconds @16:43:15.33 SS.reconfigure :exhale @16:43:15.37 SS.hold @16:43:22.84 Intake @13.5psia, vacuum from 8.0 to 11.0psi @16:43:22.86 SS.reconfigure :inhaling 0.34ml/s @16:43:22.90 SS.seek 25ml @16:43:34.36 Stroke completed -- waiting for pressure to equalize ... @16:43:34.37 SS.reconfigure :exhale @16:43:34.41 SS.hold @16:43:37.36 PBV.dial between PSV and waste1 @16:43:37.73 PSV.dial :air @16:43:38.34 PS.reconfigure :fast @16:43:38.38 PS.seek 2.5ml @16:43:47.06 Intake.select :close @16:43:47.20 SSV.dial :exhaust @16:43:47.97 Exhaust.select :open @16:43:48.08 SS.seek 0ml @16:43:56.92 Exhaust.select :close @16:43:57.50 SSV.dial CBV @16:43:57.51 CBV.dial SSV @16:43:58.28 Allowing 130s for 25.0ml of remaining 450.0ml @16:43:58.29 Intake.select :open @16:44:00.45 Intake @14.7psia, vacuum from 9.2 to 12.2psi @16:44:00.47 SS.reconfigure :inhaling 0.34ml/s @16:44:00.51 SS.seek 25ml @16:44:02.06 PSV.dial :mixloop @16:44:02.51 delay 2 seconds @16:44:04.53 PSV.dial :air @16:44:05.12 delay 2 seconds @16:44:07.14 PSV.dial :mixloop @16:44:07.59 delay 2 seconds @16:44:09.61 PSV.dial :air @16:44:10.58 PS.seek 0ml @16:44:33.80 washCycleDA #1 of 3 @16:44:33.81 PSR.select :air @16:44:33.83 PSV.dial PSR @16:44:35.22 Pulling 0.5ml of PSR.wash @16:44:35.23 PSR.select :wash @16:44:35.34 PS.reconfigure :default @16:44:35.38 PS.seek 0.5ml @16:44:41.37 delay 5 seconds @16:44:46.39 PSR.select :air @16:44:46.50 PS.seek 2ml @16:45:02.71 PTV.dial :puck @16:45:03.46 PSV.dial PTV @16:45:04.21 PBV.dial :waste1 @16:45:04.65 PS.seek 1.5ml @16:45:10.52 delay 3 seconds @16:45:13.54 PBV.dial between PSV and waste1 @16:45:13.92 PS.seek 0ml @16:45:20.78 Stroke completed -- waiting for pressure to equalize ... @16:45:20.79 SS.reconfigure :exhale @16:45:20.83 SS.hold @16:45:30.30 delay 2:00 @16:45:41.16 Intake.select :close @16:45:41.30 SSV.dial :exhaust @16:45:42.07 Exhaust.select :open @16:45:42.18 SS.seek 0ml @16:45:51.21 Exhaust.select :close @16:45:51.32 SSV.dial CBV @16:45:51.34 CBV.dial SSV @16:45:52.11 Allowing 130s for 25.0ml of remaining 425.0ml @16:45:52.12 Intake.select :open @16:45:54.27 Intake @14.7psia, vacuum from 9.2 to 12.2psi @16:45:54.29 SS.reconfigure :inhaling 0.34ml/s @16:45:54.33 SS.seek 25ml @16:47:04.19 Vacuum too high -- waiting up to 40 seconds @16:47:04.20 SS.reconfigure :exhale @16:47:04.24 SS.hold @16:47:13.89 Intake @14.1psia, vacuum from 8.6 to 11.6psi @16:47:13.91 SS.reconfigure :inhaling 0.31ml/s @16:47:13.95 SS.seek 25ml @16:47:26.24 Stroke completed -- waiting for pressure to equalize ... @16:47:26.25 SS.reconfigure :exhale @16:47:26.30 SS.hold @16:47:30.32 recoverPSRreagent08 @16:47:30.33 PTV.dial :puck @16:47:30.34 PBV.dial :waste1 @16:47:31.02 delay 30 seconds @16:47:42.66 Intake.select :close @16:47:42.80 SSV.dial :exhaust @16:47:43.56 Exhaust.select :open @16:47:43.67 SS.seek 0ml @16:47:52.46 Exhaust.select :close @16:47:52.57 SSV.dial CBV @16:47:52.59 CBV.dial SSV @16:47:53.37 Allowing 130s for 25.0ml of remaining 400.0ml @16:47:53.38 Intake.select :open @16:47:55.54 Intake @14.7psia, vacuum from 9.2 to 12.2psi @16:47:55.56 SS.reconfigure :inhaling 0.31ml/s @16:47:55.60 SS.seek 25ml @16:48:01.04 PBV.dial between PSV and waste1 @16:48:01.41 PSR.select :air @16:48:01.43 PSV.dial PSR @16:48:02.20 PS.reconfigure :fast @16:48:02.24 PS.seek 2.5ml @16:48:26.11 PSV.dial PTV @16:48:26.88 delay 5 seconds @16:48:31.90 PSV.dial PSR @16:48:32.68 delay 5 seconds @16:48:37.70 PSV.dial PTV @16:48:38.46 delay 5 seconds @16:48:43.48 PSV.dial :air @16:48:44.24 PS.seek 0ml @16:49:07.46 washCycleDA #2 of 3 @16:49:07.47 PSR.select :air @16:49:07.48 PSV.dial PSR @16:49:08.87 Pulling 0.5ml of PSR.wash @16:49:08.88 PSR.select :wash @16:49:08.99 PS.reconfigure :default @16:49:09.03 PS.seek 0.5ml @16:49:14.96 delay 5 seconds @16:49:19.98 PSR.select :air @16:49:20.09 PS.seek 2ml @16:49:25.91 Stroke completed -- waiting for pressure to equalize ... @16:49:25.92 SS.reconfigure :exhale @16:49:25.97 SS.hold @16:49:36.20 PTV.dial :puck @16:49:36.21 PSV.dial PTV @16:49:36.96 PBV.dial :waste1 @16:49:37.39 PS.seek 1.5ml @16:49:43.30 delay 3 seconds @16:49:46.31 PBV.dial between PSV and waste1 @16:49:46.70 PS.seek 0ml @16:49:50.36 Intake.select :close @16:49:50.51 SSV.dial :exhaust @16:49:51.27 Exhaust.select :open @16:49:51.38 SS.seek 0ml @16:50:00.22 Exhaust.select :close @16:50:00.33 SSV.dial CBV @16:50:00.35 CBV.dial SSV @16:50:01.12 Allowing 130s for 25.0ml of remaining 375.0ml @16:50:01.13 Intake.select :open @16:50:02.80 delay 2:00 @16:50:03.28 Intake @14.8psia, vacuum from 9.3 to 12.3psi @16:50:03.30 SS.reconfigure :inhaling 0.31ml/s @16:50:03.34 SS.seek 25ml @16:51:06.28 Vacuum too high -- waiting up to 40 seconds @16:51:06.29 SS.reconfigure :exhale @16:51:06.33 SS.hold @16:51:16.52 Intake @14.1psia, vacuum from 8.6 to 11.6psi @16:51:16.54 SS.reconfigure :inhaling 0.28ml/s @16:51:16.58 SS.seek 25ml @16:51:47.64 Stroke completed -- waiting for pressure to equalize ... @16:51:47.66 SS.reconfigure :exhale @16:51:47.70 SS.hold @16:52:02.82 recoverPSRreagent08 @16:52:02.83 PTV.dial :puck @16:52:02.84 PBV.dial :waste1 @16:52:03.20 delay 30 seconds @16:52:10.04 Intake.select :close @16:52:10.19 SSV.dial :exhaust @16:52:10.96 Exhaust.select :open @16:52:11.07 SS.seek 0ml @16:52:19.91 Exhaust.select :close @16:52:20.02 SSV.dial CBV @16:52:20.06 CBV.dial SSV @16:52:20.79 Allowing 130s for 25.0ml of remaining 350.0ml @16:52:20.80 Intake.select :open @16:52:22.96 Intake @14.9psia, vacuum from 9.4 to 12.4psi @16:52:22.98 SS.reconfigure :inhaling 0.28ml/s @16:52:23.02 SS.seek 25ml @16:52:33.23 PBV.dial between PSV and waste1 @16:52:33.59 PSR.select :air @16:52:33.61 PSV.dial PSR @16:52:34.50 PS.reconfigure :fast @16:52:34.55 PS.seek 2.5ml @16:52:58.39 PSV.dial PTV @16:52:59.14 delay 5 seconds @16:53:04.16 PSV.dial PSR @16:53:04.92 delay 5 seconds @16:53:09.94 PSV.dial PTV @16:53:10.70 delay 5 seconds @16:53:15.72 PSV.dial :air @16:53:16.49 PS.seek 0ml @16:53:39.64 washCycleDA #3 of 3 @16:53:39.65 PSR.select :air @16:53:39.67 PSV.dial PSR @16:53:41.03 Pulling 0.5ml of PSR.wash @16:53:41.04 PSR.select :wash @16:53:41.16 PS.reconfigure :default @16:53:41.20 PS.seek 0.5ml @16:53:47.08 delay 5 seconds @16:53:52.10 PSR.select :air @16:53:52.21 PS.seek 2ml @16:53:55.62 Vacuum too high -- waiting up to 40 seconds @16:53:55.63 SS.reconfigure :exhale @16:53:55.67 SS.hold @16:54:08.38 Intake @14.2psia, vacuum from 8.7 to 11.7psi @16:54:08.40 SS.reconfigure :inhaling 0.25ml/s @16:54:08.46 PTV.dial :puck @16:54:08.48 SS.seek 25ml @16:54:08.49 PSV.dial PTV @16:54:09.31 PBV.dial :waste1 @16:54:09.75 PS.seek 1.5ml @16:54:15.67 delay 3 seconds @16:54:18.53 Stroke completed -- waiting for pressure to equalize ... @16:54:18.54 SS.reconfigure :exhale @16:54:18.59 SS.hold @16:54:18.68 PBV.dial between PSV and waste1 @16:54:19.33 PS.seek 0ml @16:54:30.81 SS.reconfigure :default @16:54:30.85 SS.hold @16:54:30.97 Intake.select :close @16:54:31.12 ShallowSampler::Clogged in WCR -- Filtering too Slowly after 675.0ml @16:54:31.13 SSV.dial :exhaust @16:54:31.90 Exhaust.select :open @16:54:32.01 SS.reconfigure :exhale @16:54:32.05 SS.seek 0ml @16:54:35.48 delay 2:00 @16:54:40.91 Exhaust.select :close @16:54:41.01 Sampled 675.0ml @16:54:41.03 Drying puck before fixing for 20:00 @16:54:41.04 CBV.dial :waste2b @16:54:41.78 delay 1:00 @16:55:41.80 CSV.dial :air @16:55:42.25 CS.reconfigure :fast @16:55:42.30 CS.seek 10ml @16:56:06.73 Pumping wcr puck from tube 3 up to 35psi... @16:56:06.75 CBV.dial SSV @16:56:08.06 CBV.dial between CSV and waste2b @16:56:09.02 CSV.dial between air and CTV @16:56:09.35 CS.seek 7.399ml @16:56:16.11 CTV.dial CSV,avoiding: :air @16:56:16.13 CSV.dial CTV @16:56:16.95 CS.reconfigure :pressurize @16:56:17.00 CS.seek 0ml @16:56:31.44 CBV.dial :waste2b @16:56:31.77 CS.reconfigure :default @16:56:31.81 CS.seek 0ml @16:56:35.50 recoverPSRreagent08 @16:56:35.51 PTV.dial :puck @16:56:35.52 PBV.dial :waste1 @16:56:35.89 delay 30 seconds @16:56:37.76 delay 1:00 @16:57:05.91 PBV.dial between PSV and waste1 @16:57:06.27 PSR.select :air @16:57:06.29 PSV.dial PSR @16:57:07.06 PS.reconfigure :fast @16:57:07.10 PS.seek 2.5ml @16:57:31.04 PSV.dial PTV @16:57:31.81 delay 5 seconds @16:57:36.83 PSV.dial PSR @16:57:37.57 delay 5 seconds @16:57:37.78 CTV.dial :air,avoiding: :intake @16:57:39.11 Fix #1 of 2 (with RNAlater) for 10:00 @16:57:39.12 CSV.dial CSR @16:57:39.61 Pulling 1.999ml of CSR.RNAlater @16:57:39.62 CSR.select :RNAlater @16:57:39.73 CS.seek 2ml @16:57:42.59 PSV.dial PTV @16:57:43.35 delay 5 seconds @16:57:45.89 delay 5 seconds @16:57:48.37 PSV.dial :air @16:57:49.25 PS.seek 0ml @16:57:50.90 CSR.select :air @16:57:51.01 CS.reconfigure :fast @16:57:51.06 CS.seek 10ml @16:58:10.73 Pumping wcr puck from tube 3 up to 35psi... @16:58:10.74 CBV.dial SSV @16:58:12.45 CBV.dial between CSV and waste2b @16:58:12.52 Gate.power :camera,:ON @16:58:12.53 Mixing DA Substrates @16:58:12.54 PSR.select :air @16:58:12.55 PSV.dial PSR @16:58:13.47 CSV.dial between air and CTV @16:58:14.12 Pulling 0.25ml of PSR.sub1 @16:58:14.14 PSR.select :sub1 @16:58:14.33 CS.seek 7.259ml @16:58:14.49 PS.reconfigure :default @16:58:14.59 PS.seek 0.25ml @16:58:18.74 delay 5 seconds @16:58:21.49 CTV.dial CSV,avoiding: :air @16:58:21.51 CSV.dial CTV @16:58:22.92 CS.reconfigure :pressurize @16:58:22.97 CS.seek 0ml @16:58:23.81 Pulling 0.25ml of PSR.sub2 @16:58:23.82 PSR.select :sub2 @16:58:23.93 PS.seek 0.5ml @16:58:27.35 delay 5 seconds @16:58:32.37 PSR.select :air @16:58:32.48 PS.seek 2ml @16:58:40.97 delay 10:00 @16:58:48.60 PTV.dial :puck @16:58:48.61 PSV.dial PTV @16:58:49.49 PBV.dial :waste1 @16:58:49.92 PS.seek 1.5ml @16:58:55.76 delay 3 seconds @16:58:58.77 PBV.dial between PSV and waste1 @16:58:59.15 PS.seek 0ml @16:59:15.35 delay 10 seconds @16:59:25.38 recoverPSRreagent08 PTV.dial :puck @16:59:25.39 PBV.dial :waste1 @16:59:25.76 delay 30 seconds @16:59:55.78 PBV.dial between PSV and waste1 @16:59:56.15 PSR.select :air @16:59:56.16 PSV.dial PSR @16:59:56.92 PS.reconfigure :fast @16:59:56.96 PS.seek 2.5ml @17:00:21.00 PSV.dial PTV @17:00:21.77 delay 5 seconds @17:00:26.79 PSV.dial PSR @17:00:27.55 delay 5 seconds @17:00:32.57 PSV.dial PTV @17:00:33.44 delay 5 seconds @17:00:38.46 PSV.dial :air @17:00:39.24 PS.seek 0ml @17:01:02.78 Additional air clear @17:01:02.79 PSR.select :air @17:01:02.81 PSV.dial PSR @17:01:04.20 Pulling 2ml of PSR.air @17:01:04.21 PSR.select :air @17:01:04.23 PS.reconfigure :default @17:01:04.27 PS.seek 2ml @17:01:25.50 delay 5 seconds @17:01:30.52 PTV.dial :puck @17:01:30.54 PSV.dial PTV @17:01:31.30 PBV.dial :waste1 @17:01:31.72 PS.seek 1.5ml @17:01:37.63 delay 3 seconds @17:01:40.65 PBV.dial between PSV and waste1 @17:01:41.02 delay 1 second @17:01:42.04 PSV.dial :waste1 @17:01:43.11 PS.seek 0ml @17:01:59.30 Processing Puck Heater at 28C before imaging @17:01:59.31 PH.coast @17:01:59.43 Imaging da puck in Processing Clamp while storing it to tube 2 @17:01:59.59 SC.reconfigure :tight @17:01:59.64 SC.seek 2 @17:02:03.18 SC.configure :limp @17:02:03.37 Elbow.reconfigure :tight @17:02:03.41 Elbow.seek 2 @17:02:05.20 Forearm.reconfigure :tight @17:02:05.25 Forearm.seek 2 + 450 counts @17:02:06.26 SE.reconfigure :pick @17:02:06.31 SE.seek :beamBreak @17:02:08.88 SE.configure :default @17:02:09.03 Elbow.reconfigure :limp @17:02:09.07 Forearm.reconfigure :loose @17:02:09.11 Forearm.seek 2 @17:02:09.28 Elbow.hold @17:02:09.34 Elbow.reconfigure :tight @17:02:09.43 SE.seek 8.12 Pucks below Top Plate @17:02:09.89 Forearm.force -20 @17:02:09.93 Forearm.seek 2 + 314 counts @17:02:10.33 Forearm.reconfigure :tight @17:02:10.37 Forearm.seek 2 + 450 counts @17:02:10.58 SE.seek 9.12 Pucks below Top Plate @17:02:11.09 Forearm.reconfigure :loose @17:02:11.14 Forearm.seek :retract @17:02:12.08 Elbow.seek PC @17:02:14.82 Elbow.reconfigure :loose @17:02:14.90 Forearm.seek PC @17:02:15.87 Hand.close @17:02:16.48 PC.seek :open @17:02:21.22 Forearm.seek :retract @17:02:22.22 Elbow.reconfigure :tight @17:02:22.71 Elbow.seek 2 @17:02:25.39 Forearm.reconfigure :tight @17:02:25.44 Forearm.seek 2 + 450 counts @17:02:26.46 Hand.open @17:02:27.17 SE.seek 8.78 Pucks below Top Plate @17:02:27.45 SE.reconfigure :seal @17:02:27.49 SE.seek 7.81 Pucks below Top Plate @17:02:28.03 Lights, camera, ACTION! @17:02:28.06 PuckCamera: snap -bin=2,2 -auto /var/log/esp/da23jan1717h575ml.tif @17:02:47.54 Waiting >20 seconds for da to relinquish FlushPuck @17:02:51.42 SX HX9/16 (USB:11): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 20.089 seconds /var/log/esp/da23jan1717h575ml.tif: TIFF Upload Complete @17:02:51.45 PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da23jan1717h575ml15s.tif @17:03:07.06 SX HX9/16 (USB:11): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15 seconds /var/log/esp/midres/da23jan1717h575ml15s.tif: TIFF Upload Complete @17:03:07.09 PuckCamera: snap -bin=2,2 20.0 /var/log/esp/midres/da23jan1717h575ml20s.tif @17:03:27.71 SX HX9/16 (USB:11): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 20 seconds /var/log/esp/midres/da23jan1717h575ml20s.tif: TIFF Upload Complete @17:03:27.73 DAfans.email "20.089 sec auto-exposure",Subject: "da23jan1717h575ml images!" @17:03:27.76 Gate.power :camera,:OFF @17:03:28.00 Elbow.seek 2 @17:03:28.20 Forearm.seek 2 + 450 counts @17:03:28.28 SE.reconfigure :gently @17:03:28.32 SE.seek 8.53 Pucks below Top Plate @17:03:28.98 Elbow.reconfigure :limp @17:03:29.03 Forearm.reconfigure :loose @17:03:29.07 Forearm.seek 2 @17:03:29.30 Elbow.hold @17:03:29.38 Elbow.reconfigure :tight @17:03:29.43 SE.reconfigure :default @17:03:29.47 SE.seek 8.71 Pucks below Top Plate @17:03:29.95 Forearm.force -20 @17:03:29.99 Forearm.seek 2 + 304 counts @17:03:30.33 Forearm.reconfigure :tight @17:03:30.38 Forearm.seek 2 + 450 counts @17:03:30.65 SE.seek :down @17:03:31.78 Forearm.reconfigure :loose @17:03:31.83 Forearm.seek :retract @17:03:32.79 SE.stop @17:03:33.02 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @17:03:33.40 Elbow.seek FlushPuck::Garage @17:03:35.40 Elbow.reconfigure :loose @17:03:35.61 Forearm.seek :garage @17:03:36.65 Hand.close @17:03:37.51 Forearm.seek :retract @17:03:38.55 Elbow.reconfigure :tight @17:03:38.68 Elbow.seek PC @17:03:40.08 Elbow.reconfigure :loose @17:03:40.26 Forearm.reconfigure :tight @17:03:40.34 Forearm.seek PC @17:03:41.87 PC.seek :closed @17:03:46.68 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.58 @17:03:46.85 Hand.open @17:03:47.63 Forearm.reconfigure :loose @17:03:47.70 Forearm.seek :retract @17:03:48.71 PSR.select :air @17:03:48.75 PSV.dial PSR @17:03:49.36 Pulling 0.5ml of PSR.flush @17:03:49.38 PSR.select :flush @17:03:49.55 PS.seek 0.5ml @17:03:56.75 delay 5 seconds @17:04:01.78 PSR.select :air @17:04:01.89 PS.seek 2.5ml @17:04:02.05 upsync @17:04:23.09 delay 1 second @17:04:24.11 PRV.dial :waste2,avoiding: :ab2 @17:04:25.98 PTV.dial :PRVmixing @17:04:27.31 PSV.dial :mixloop @17:04:28.37 PS.seek :empty @17:04:54.72 PSR.select :air @17:04:54.74 PSV.dial PSR @17:04:55.85 Pulling 0.5ml of PSR.flush @17:04:55.86 PSR.select :flush @17:04:55.98 PS.seek 0.5ml @17:05:02.00 delay 5 seconds @17:05:07.02 PSR.select :air @17:05:07.14 PS.seek 2.5ml @17:05:28.31 delay 1 second @17:05:29.33 PBV.dial :waste1 @17:05:29.70 PTV.dial :mixing @17:05:30.45 PSV.dial :mixloop @17:05:31.52 PS.seek :empty @17:05:58.09 PSV.dial PSR @17:05:59.14 PSR.select :flush @17:05:59.26 PS.seek 1ml @17:06:10.24 PSR.select :air @17:06:10.36 PS.seek 2.5ml @17:06:26.53 PSV.dial PTV @17:06:27.29 PTV.dial :puck @17:06:28.04 PBV.dial :waste1 @17:06:28.06 PS.seek 0ml @17:06:54.43 PSV.dial PSR @17:06:55.20 PSR.select :flush @17:06:55.32 PS.seek 0.5ml @17:07:01.28 PSR.select :air @17:07:01.39 PS.seek 2.5ml @17:07:22.62 PSV.dial PTV @17:07:23.39 PBV.dial PSV @17:07:23.96 PS.seek 0ml @17:07:50.53 PSV.dial PBV @17:07:51.00 PS.seek 2.5ml @17:08:17.40 PSV.dial :waste1 @17:08:18.16 PS.seek 0ml @17:08:40.99 CBV.dial :waste2b @17:08:41.32 CS.reconfigure :default @17:08:41.36 CS.seek 0ml @17:08:43.22 delay 1:00 @17:08:44.52 PSV.dial PSR @17:08:44.99 PSR.select :air @17:08:45.02 PS.seek 2.5ml @17:09:11.43 PSV.dial PTV @17:09:12.17 PBV.dial PSV @17:09:12.19 PS.seek 0ml @17:09:38.74 PSV.dial PBV @17:09:39.22 PS.seek 2.5ml @17:09:43.24 CTV.dial :air,avoiding: :intake @17:09:44.57 Fix #2 of 2 (with RNAlater) for 10:00 @17:09:44.58 CSV.dial CSR @17:09:45.07 Pulling 2ml of CSR.RNAlater @17:09:45.08 CSR.select :RNAlater @17:09:45.19 CS.seek 2ml @17:09:51.19 delay 5 seconds @17:09:56.21 CSR.select :air @17:09:56.32 CS.reconfigure :fast @17:09:56.36 CS.seek 10ml @17:10:05.59 PSV.dial :waste1 @17:10:06.33 PS.seek 0ml @17:10:15.94 Pumping wcr puck from tube 3 up to 35psi... @17:10:15.95 CBV.dial SSV @17:10:17.25 CBV.dial between CSV and waste2b @17:10:18.20 CSV.dial between air and CTV @17:10:18.80 CS.seek 7.17ml @17:10:26.00 CTV.dial CSV,avoiding: :air @17:10:26.03 CSV.dial CTV @17:10:27.39 CS.reconfigure :pressurize @17:10:27.44 CS.seek 0ml @17:10:32.74 PSV.dial PSR @17:10:33.21 PSR.select :flush @17:10:33.33 PS.seek 1ml @17:10:42.95 delay 10:00 @17:10:44.43 PSR.select :air @17:10:44.54 PS.seek 2.5ml @17:11:00.73 PSV.dial PTV @17:11:01.46 PTV.dial :puck @17:11:01.48 PBV.dial :waste1 @17:11:02.05 PS.seek 0ml @17:11:28.43 PSV.dial PSR @17:11:29.18 PSR.select :flush @17:11:29.30 PS.seek 0.5ml @17:11:35.18 PSR.select :air @17:11:35.29 PS.seek 2.5ml @17:11:56.55 PSV.dial PTV @17:11:57.32 PBV.dial PSV @17:11:57.89 PS.seek 0ml @17:12:24.27 PSV.dial PBV @17:12:24.75 PS.seek 2.5ml @17:12:51.11 PSV.dial :waste1 @17:12:51.87 PS.seek 0ml @17:13:18.21 PSV.dial PSR @17:13:18.68 PSR.select :air @17:13:18.70 PS.seek 2.5ml @17:13:45.21 PSV.dial PTV @17:13:45.94 PBV.dial PSV @17:13:45.96 PS.seek 0ml @17:14:12.30 PSV.dial PBV @17:14:12.78 PS.seek 2.5ml @17:14:39.17 PSV.dial :waste1 @17:14:39.91 PS.seek 0ml @17:15:06.30 Garaging FlushPuck in Processing Clamp @17:15:06.37 Elbow.reconfigure :tight @17:15:06.42 Elbow.seek PC @17:15:06.53 Elbow.reconfigure :loose @17:15:06.62 Forearm.seek PC @17:15:07.56 Hand.close @17:15:08.22 PC.seek :open @17:15:12.94 Forearm.seek :retract @17:15:13.91 Elbow.reconfigure :tight @17:15:13.95 Elbow.seek :garage @17:15:15.31 Elbow.reconfigure :loose @17:15:15.40 Forearm.reconfigure :tight @17:15:15.44 Forearm.seek :garage @17:15:16.45 Hand.open @17:15:17.15 Forearm.reconfigure :loose @17:15:17.20 Forearm.seek :retract @17:20:42.97 CBV.dial :waste2b @17:20:43.30 CS.reconfigure :default @17:20:43.35 CS.seek 0ml @17:20:47.33 delay 1:00 @17:21:47.36 CTV.dial :air,avoiding: :intake @17:21:48.75 Storing wcr puck in Collection Clamp to tube 2 @17:21:48.91 SC.reconfigure :tight @17:21:48.95 SC.seek 2 @17:21:49.01 SC.configure :limp @17:21:49.20 Elbow.reconfigure :tight @17:21:49.25 Elbow.seek 2 @17:21:51.20 Forearm.reconfigure :tight @17:21:51.25 Forearm.seek 2 + 450 counts @17:21:52.29 SE.reconfigure :pick @17:21:52.35 SE.seek :beamBreak @17:21:54.88 SE.configure :default @17:21:55.03 Elbow.reconfigure :limp @17:21:55.09 Forearm.reconfigure :loose @17:21:55.14 Forearm.seek 2 @17:21:55.31 Elbow.hold @17:21:55.38 Elbow.reconfigure :tight @17:21:55.47 SE.seek 9.13 Pucks below Top Plate @17:21:55.94 Forearm.force -20 @17:21:55.98 Forearm.seek 2 + 302 counts @17:21:56.27 Forearm.reconfigure :tight @17:21:56.31 Forearm.seek 2 + 450 counts @17:21:56.53 SE.seek 10.13 Pucks below Top Plate @17:21:57.59 Forearm.reconfigure :loose @17:21:57.65 Forearm.seek :retract @17:21:58.58 Elbow.seek CC @17:22:00.19 Elbow.reconfigure :loose @17:22:00.28 Forearm.seek CC @17:22:01.24 Hand.close @17:22:01.84 CC.seek :open @17:22:06.58 Forearm.seek :retract @17:22:07.55 Elbow.reconfigure :tight @17:22:07.59 Elbow.seek 2 @17:22:09.39 Elbow.reconfigure :limp @17:22:09.44 Forearm.seek 2 @17:22:10.40 Elbow.hold @17:22:10.47 Elbow.reconfigure :tight @17:22:10.52 Hand.open @17:22:11.20 Forearm.force -20 @17:22:11.24 Forearm.seek 2 + 307 counts @17:22:11.52 Forearm.reconfigure :tight @17:22:11.57 Forearm.seek 2 + 450 counts @17:22:11.86 SE.seek :down @17:22:12.98 Forearm.reconfigure :loose @17:22:13.03 Forearm.seek :retract @17:22:13.87 SE.stop @17:22:13.97
Starting finalPurge @17:22:13.98 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @17:22:14.16 Elbow.seek FlushPuck::Garage @17:22:16.01 Elbow.reconfigure :loose @17:22:16.10 Forearm.seek :garage @17:22:17.04 Hand.close @17:22:17.70 Forearm.seek :retract @17:22:18.64 Elbow.reconfigure :tight @17:22:18.68 Elbow.seek CC @17:22:21.63 Elbow.reconfigure :loose @17:22:21.72 Forearm.reconfigure :tight @17:22:21.76 Forearm.seek CC @17:22:22.94 CC.seek :closed @17:22:27.45 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.33 @17:22:27.52 Hand.open @17:22:28.15 Forearm.reconfigure :loose @17:22:28.19 Forearm.seek :retract @17:22:29.20 Flushing the Collection Valve::Manifold @17:22:29.32 CSV.dial CSR @17:22:29.80 Pulling 5ml of CSR.flush @17:22:29.81 CSR.select :flush @17:22:29.92 CS.seek 5ml @17:22:43.74 delay 5 seconds @17:22:48.75 CSR.select :air @17:22:48.90 CS.reconfigure :fast @17:22:48.94 CS.seek 7.5ml @17:22:55.86 CSV.dial :waste @17:22:57.13 CS.reconfigure :default @17:22:57.17 CS.seek 0ml @17:23:17.14 Flushing lower manifold's waste1 port @17:23:17.25 CSV.dial :flush @17:23:17.69 CS.reconfigure :fast @17:23:17.73 CS.seek 5ml @17:23:30.54 delay 5 seconds @17:23:35.56 CSV.dial :air @17:23:36.18 CS.seek 7ml @17:23:41.64 CSV.dial CTV @17:23:41.66
CTV.dial CSV @17:23:43.06 CBV.dial :waste1 @17:23:43.82 CS.seek 0ml @17:24:00.39 Flushing lower manifold's waste2 port @17:24:01.00 CSV.dial :flush @17:24:01.71 CS.seek 5ml @17:24:14.61 delay 5 seconds @17:24:19.63 CSV.dial :air @17:24:20.14 CS.seek 7ml @17:24:25.64 CSV.dial CTV @17:24:25.66
CTV.dial CSV @17:24:26.11 CBV.dial :waste2 @17:24:27.14 CS.seek 0ml @17:24:43.55 Flushing lower manifold's waste2b port @17:24:43.67 CSV.dial :flush @17:24:44.50 CS.seek 5ml @17:24:57.36 delay 5 seconds @17:25:02.37 CSV.dial :air @17:25:02.87 CS.seek 7ml @17:25:08.33 CSV.dial CTV @17:25:08.38
CTV.dial CSV @17:25:08.78 CBV.dial :waste2b @17:25:09.83 CS.seek 0ml @17:25:26.29 Disinfecting external Intake with kill @17:25:26.41 CSV.dial CSR @17:25:27.02 Pulling 8ml of CSR.kill @17:25:27.03 CSR.select :kill @17:25:27.14 CS.seek 8ml @17:25:47.01 delay 5 seconds @17:25:52.03 CSR.select :air @17:25:52.14 CS.seek 10ml @17:25:57.64 CSV.dial CBV @17:25:57.66
CBV.dial CSV @17:25:58.72 CTV.dial :intake @17:25:59.49 Intake.select :open @17:25:59.60 CS.seek 2ml @17:26:18.41 Intake.select :close @17:26:18.52 CTV.dial :air @17:26:19.28 CS.reconfigure :default @17:26:19.32 CS.seek 0ml @17:26:25.23 CSV.dial CSR @17:26:26.28 Pulling 5ml of CSR.flush @17:26:26.29 CSR.select :flush @17:26:26.40 CS.seek 5ml @17:26:40.01 delay 5 seconds @17:26:45.03 CSR.select :air @17:26:45.14 CS.seek 10ml @17:26:58.79 CSV.dial CBV @17:26:59.78 CS.seek 0ml @17:27:26.20 Garaging FlushPuck in Collection Clamp @17:27:26.27 Elbow.reconfigure :tight @17:27:26.31 Elbow.seek CC @17:27:26.58 Elbow.reconfigure :loose @17:27:26.66 Forearm.seek CC @17:27:27.61 Hand.close @17:27:28.21 CC.seek :open @17:27:32.84 Forearm.seek :retract @17:27:33.84 Elbow.reconfigure :tight @17:27:33.88 Elbow.seek :garage @17:27:36.79 Elbow.reconfigure :loose @17:27:36.88 Forearm.reconfigure :tight @17:27:36.92 Forearm.seek :garage @17:27:37.93 Hand.open @17:27:38.64 Forearm.reconfigure :loose @17:27:38.68 Forearm.seek :retract @17:27:39.63 Completed [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] begun 15:02:45 @17:27:39.68 Asleep after completing DA_WCR_11723 assays @17:27:39.70 GoodNews.email "{1 => 33,\n 2 => 11,\n 3 => 22,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@17:21:55, 18% flow, 17.8C, 28% humidity, 14.6psia, 13.710V, 0.520A, 0.502A avg, 7.13W\nSampled 675.0ml",Subject: "Asleep after completing DA_WCR_11723 assays" @17:27:45.12 upsync @15:52:17.32UTC23-Jan-23
ESP2 v3.41 I2C Gateway v3.20 for core bus @15:52:19.89 Configuration for short puck jake 1/11/21 by brent@mbari.org /home/esp/esp2/type/shallow/jake/configure.rb @15:52:23.79 Polling Can every 10:00 @15:52:23.91 Cleanly exited at 15:49:07UTC23-Jan-23 @15:52:23.94 Loading /home/esp/esp2/mission/DA_WCR_12323.rb mission @15:52:23.99 Preparing to sleep until + 00:00:05 @15:52:24.03 GoodNews.email "Starting DA_WCR_12323 w/pucks in tube 3\n{1 => 33,\n 2 => 11,\n 3 => 22,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@15:52:23, 21% flow, 12.3C, 35% humidity, 14.4psia, 13.754V, 0.327A, 0.315A avg, 4.49W",Subject: "DA_WCR_12323 begun" @15:52:24.07
Asleep til + 00:00:05 @15:52:24.14 Continuing at 15:52 @15:52:24.34 Gate.power :core,:OFF @15:52:24.35 Gate.power :raw,:OFF @15:52:24.36 Shutdown @15:52:28.13 GoodNews.email "Then, in tube 3: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2/mission/DA_WCR_12323.rb:14\n{1 => 33,\n 2 => 11,\n 3 => 22,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@15:52:24, 21% flow, 12.3C, 35% humidity, 14.4psia, 13.754V, 0.327A, 0.315A avg, 4.49W",Subject: "Asleep until 15:52" @15:52:28.97
Powering core bus @15:52:29.93 upsync @15:52:33.39 upsync @15:52:34.10
Gate.power :raw,:ON @15:52:34.83 CS.configure :default @15:52:34.92 PS.configure :default @15:52:35.00 SS.configure :default @15:52:35.09 CC.configure :default @15:52:35.18 PC.configure :default @15:52:35.29 Forearm.configure :loose @15:52:35.37 Elbow.configure :loose @15:52:35.48 SC.configure :loose @15:52:35.57 SE.configure :default @15:52:35.61 CH.coast @15:52:35.66 CH.configure :default @15:52:35.69 PH.coast @15:52:35.74 PH.configure :default @15:52:35.78 All dwarf controllers running firmware version 3.85 @15:52:35.88 Valve.reset @15:52:36.07 Intake.select :close @15:52:36.12 Exhaust.select :close @15:52:36.18 CTV.dial :air @15:52:36.27 PSV.dial :air @15:52:36.29 CSV.dial :waste1 @15:52:36.42
Forearm.reconfigure :loose @15:52:36.46 Forearm.home.seek :retract @15:52:36.80 SSV.dial :spit,avoiding: :exhaust @15:52:37.18 Hand.close @15:52:37.20
Elbow.jog 1500 @15:52:37.41 PS.reconfigure :default @15:52:37.46 PS.home.seek 0ml @15:52:37.62 CS.reconfigure :fast @15:52:37.66 SS.reconfigure :default @15:52:37.74 CS.home.seek 0ml @15:52:37.78 SS.home.seek 0ml @15:52:37.88
Elbow.reconfigure :loose @15:52:37.92 Elbow.home.seek :clear @15:52:42.58 SE.reconfigure :default @15:52:42.62 SE.home.seek :down @15:52:43.22 SC.reconfigure :tight @15:52:43.26 SC.home.seek :initial @15:52:45.65 PS.stop @15:53:11.57
SC.configure :limp @15:53:11.62 Forearm.seek :retract @15:53:11.68 Elbow.seek :hammer @15:53:12.74 Forearm.reconfigure :tight @15:53:12.78 Forearm.seek retracted @15:53:13.06 PC.reconfigure :default @15:53:13.08
CC.reconfigure :default @15:53:13.16 PC.home.seek :open @15:53:13.19
CC.home.seek :open @15:53:16.69 Phase [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] @15:53:16.70 Notice.email "At 15:53:16 began in tube 3: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2/mission/DA_WCR_12323.rb:14\n{1 => 33,\n 2 => 11,\n 3 => 22,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@15:53:16, 21% flow, 12.3C, 35% humidity, 14.4psia, 13.759V, 0.290A, 0.272A avg, 4.00W",Subject: "Started DA_WCR_12323 phase initialPurge; da [1000, 1000] #da and wsr are run simultanelously" @15:53:16.72
Starting initialPurge @15:53:16.75 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @15:53:16.81 Hand.open @15:53:17.53 Elbow.reconfigure :tight @15:53:17.59 Elbow.seek FlushPuck::Garage @15:53:19.56 Elbow.reconfigure :loose @15:53:19.65 Forearm.reconfigure :loose @15:53:19.69 Forearm.seek :garage @15:53:20.66 Hand.close @15:53:21.43 Forearm.seek :retract @15:53:22.42 Elbow.reconfigure :tight @15:53:22.46 Elbow.seek CC @15:53:22.62 upsync @15:53:25.56
Elbow.reconfigure :loose @15:53:25.65 Forearm.reconfigure :tight @15:53:25.70 Forearm.seek CC @15:53:26.95 CC.seek :closed @15:53:31.57 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.31 @15:53:31.64 Hand.open @15:53:32.35 Forearm.reconfigure :loose @15:53:32.39 Forearm.seek :retract @15:53:33.35 Flushing the Collection Valve::Manifold @15:53:33.47 CSV.dial CSR @15:53:34.80 Pulling 5ml of CSR.flush @15:53:34.81 CSR.select :flush @15:53:34.92 CS.reconfigure :default @15:53:34.96 CS.seek 5ml @15:53:48.77 delay 5 seconds @15:53:53.79 CSR.select :air @15:53:53.94 CS.reconfigure :fast @15:53:53.98 CS.seek 7.5ml @15:54:00.83 CSV.dial :waste @15:54:02.10 CS.reconfigure :default @15:54:02.14 CS.seek 0ml @15:54:22.17 Purging external Intake @15:54:22.29 CSV.dial :flush @15:54:22.72 CS.reconfigure :fast @15:54:22.77 CS.seek 10ml @15:54:47.08 delay 5 seconds @15:54:52.10 CSV.dial CBV @15:54:52.13
CBV.dial CSV @15:54:52.89 CTV.dial :intake @15:54:53.70 Intake.select :open @15:54:53.81 CS.seek 0ml @15:55:17.14 Intake.select :close @15:55:17.24 CTV.dial :air @15:55:18.03 Flushing upper manifold @15:55:18.15 CSV.dial :flush @15:55:18.88 CS.seek 5ml @15:55:31.67 delay 5 seconds @15:55:36.69 CSV.dial :air @15:55:37.18 CS.seek 7ml @15:55:42.70 CSV.dial CBV @15:55:42.73
CBV.dial CSV @15:55:43.73 CTV.dial :air @15:55:43.75 CS.seek 0ml @15:56:00.61 delay 7 seconds @15:56:07.63 CS.seek 3ml @15:56:15.54 CSV.dial :waste @15:56:15.99 CS.seek 0ml @15:56:23.90 CSV.dial :flush @15:56:24.46 CS.seek 3ml @15:56:32.42 delay 5 seconds @15:56:37.44 CSV.dial :air @15:56:38.07 CS.seek 5.001ml @15:56:43.76 CSV.dial :air @15:56:43.78 CS.seek 0ml @15:56:56.26 delay 7 seconds @15:57:03.29 CS.seek 3ml @15:57:11.42 CSV.dial :waste @15:57:12.13 CS.seek 0ml @15:57:19.95 Garaging FlushPuck in Collection Clamp @15:57:20.02 Elbow.reconfigure :tight @15:57:20.06 Elbow.seek CC @15:57:20.48 Elbow.reconfigure :loose @15:57:20.57 Forearm.seek CC @15:57:21.53 Hand.close @15:57:22.16 CC.seek :open @15:57:26.81 Forearm.seek :retract @15:57:27.80 Elbow.reconfigure :tight @15:57:27.84 Elbow.seek :garage @15:57:30.79 Elbow.reconfigure :loose @15:57:30.88 Forearm.reconfigure :tight @15:57:30.92 Forearm.seek :garage @15:57:31.91 Hand.open @15:57:32.97 Forearm.reconfigure :loose @15:57:33.01 Forearm.seek :retract @15:57:33.97 da sampling at most 1000ml, wcr at most 1000ml @15:57:34.01 Loading sh1 puck from tube 3 into Collection Clamp @15:57:34.02 DAfans.email "Started da in tube 3 at 15:57:33UTC23-Jan-23\n{1 => 33,\n 2 => 11,\n 3 => 22,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@15:53:16, 21% flow, 12.3C, 35% humidity, 14.4psia, 13.759V, 0.290A, 0.272A avg, 4.00W",Subject: "da sampling at most 1000ml, wcr at most 1000ml" @15:57:34.28
SC.reconfigure :tight @15:57:34.32 SC.seek 3 @15:57:37.91 SC.configure :limp @15:57:38.13 Elbow.reconfigure :tight @15:57:38.18 Elbow.seek 3 @15:57:39.47 upsync @15:57:40.17
Forearm.reconfigure :tight @15:57:40.21 Forearm.seek 3 + 450 counts @15:57:41.25 SE.reconfigure :pick @15:57:41.31 SE.seek :beamBreak @15:57:43.13 SE.configure :default @15:57:43.28 Elbow.seek 3 @15:57:43.45 Forearm.seek 3 + 300 counts @15:57:43.63 Forearm.reconfigure :loose @15:57:43.67 Hand.close @15:57:44.30 SE.seek :down @15:57:44.50
Forearm.seek :retract @15:57:45.47 Elbow.seek CC @15:57:45.67 SE.stop @15:57:47.14
Elbow.reconfigure :loose @15:57:47.23 Forearm.reconfigure :tight @15:57:47.27 Forearm.seek CC @15:57:48.52 CC.seek :closed @15:57:53.04 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.24 @15:57:53.05 Hand.open @15:57:53.72 Forearm.reconfigure :loose @15:57:53.76 Forearm.seek :retract @15:57:54.72 collectSample 1000ml w/bubble point of 24psi @15:57:54.74 Priming 7ml Intake.select :close @15:57:54.75 Exhaust.select :close @15:57:54.77 SS.hold @15:57:54.96 Calibrating pressure and temperature sensors @15:57:54.99 CTV.dial :air @15:57:55.00 SSV.dial :spit,avoiding: :exhaust @15:57:55.02 SS.reconfigure :fast @15:57:55.06 SS.seek 5ml @15:57:57.78 SSV.dial CBV @15:57:57.81
CBV.dial SSV @15:57:59.10 delay 3.8 seconds @15:58:03.01 SSV.dial :spit @15:58:04.35 SS.seek 0ml @15:58:07.02 SSV.dial CBV @15:58:08.32 CTV.dial :intake @15:58:09.11 SSV.dial CBV @15:58:09.13
CBV.dial SSV @15:58:09.14 Allowing 40s for 7.0ml of remaining 7.0ml @15:58:09.15 Intake.select :open @15:58:11.31 Intake @14.8psia, vacuum from 9.3 to 12.3psi @15:58:11.32 SS.reconfigure :inhaling 2.20ml/s @15:58:11.37 SS.seek 7ml @15:58:17.63 Stroke completed -- waiting for pressure to equalize ... @15:58:17.64 SS.reconfigure :exhale @15:58:17.68 SS.hold @15:58:21.83 Intake.select :close @15:58:21.97 SSV.dial :exhaust @15:58:22.71 Exhaust.select :open @15:58:22.82 SS.seek 0ml @15:58:26.88 Exhaust.select :close @15:58:26.98 Sampling 1000.0ml @15:58:26.99 Intake.select :close @15:58:27.00 Exhaust.select :close @15:58:27.01 SS.reconfigure :default @15:58:27.05 SS.hold @15:58:27.23 CTV.dial :intake @15:58:27.25 SSV.dial CBV @15:58:27.27
CBV.dial SSV @15:58:28.04 Allowing 130s for 25.0ml of remaining 1000.0ml @15:58:28.05 Intake.select :open @15:58:30.20 Intake @15.3psia, vacuum from 9.8 to 12.8psi @15:58:30.21 SS.reconfigure :inhaling 2.20ml/s @15:58:30.26 SS.seek 25ml @15:58:37.39 Vacuum too high -- waiting up to 40 seconds @15:58:37.40 SS.reconfigure :exhale @15:58:37.44 SS.hold @15:58:38.30 Intake @12.5psia, vacuum from 7.0 to 10.0psi @15:58:38.32 SS.reconfigure :inhaling 1.98ml/s @15:58:38.36 SS.seek 25ml @15:58:41.89 Vacuum too high -- waiting up to 40 seconds @15:58:41.90 SS.reconfigure :exhale @15:58:41.94 SS.hold @15:58:47.05 Intake @14.1psia, vacuum from 8.6 to 11.6psi @15:58:47.06 SS.reconfigure :inhaling 1.79ml/s @15:58:47.11 SS.seek 25ml @15:58:53.06 Stroke completed -- waiting for pressure to equalize ... @15:58:53.07 SS.reconfigure :exhale @15:58:53.11 SS.hold @15:58:58.75 Intake.select :close @15:58:58.89 SSV.dial :exhaust @15:58:59.65 Exhaust.select :open @15:58:59.75 SS.seek 0ml @15:59:08.27 Exhaust.select :close @15:59:08.37 SSV.dial CBV @15:59:08.40
CBV.dial SSV @15:59:09.16 Allowing 130s for 25.0ml of remaining 975.0ml @15:59:09.17 Intake.select :open @15:59:11.32 Intake @15.1psia, vacuum from 9.6 to 12.6psi @15:59:11.34 SS.reconfigure :inhaling 1.79ml/s @15:59:11.38 SS.seek 25ml @15:59:20.48 Vacuum too high -- waiting up to 40 seconds @15:59:20.49 SS.reconfigure :exhale @15:59:20.53 SS.hold @15:59:23.72 Intake @13.2psia, vacuum from 7.7 to 10.7psi @15:59:23.74 SS.reconfigure :inhaling 1.61ml/s @15:59:23.78 SS.seek 25ml @15:59:29.95 Vacuum too high -- waiting up to 40 seconds @15:59:29.96 SS.reconfigure :exhale @15:59:30.00 SS.hold @15:59:36.65 Intake @14.2psia, vacuum from 8.7 to 11.7psi @15:59:36.66 SS.reconfigure :inhaling 1.45ml/s @15:59:36.70 SS.seek 25ml @15:59:41.41 Stroke completed -- waiting for pressure to equalize ... @15:59:41.42 SS.reconfigure :exhale @15:59:41.46 SS.hold @15:59:46.22 Intake.select :close @15:59:46.36 SSV.dial :exhaust @15:59:47.11 Exhaust.select :open @15:59:47.22 SS.seek 0ml @15:59:55.89 Exhaust.select :close @15:59:56.00 SSV.dial CBV @15:59:56.03
CBV.dial SSV @15:59:56.77 Allowing 130s for 25.0ml of remaining 950.0ml @15:59:56.78 Intake.select :open @15:59:58.93 Intake @15.1psia, vacuum from 9.6 to 12.6psi @15:59:58.95 SS.reconfigure :inhaling 1.45ml/s @15:59:58.99 SS.seek 25ml @16:00:09.54 Vacuum too high -- waiting up to 40 seconds @16:00:09.55 SS.reconfigure :exhale @16:00:09.59 SS.hold @16:00:14.57 Intake @13.7psia, vacuum from 8.2 to 11.2psi @16:00:14.58 SS.reconfigure :inhaling 1.30ml/s @16:00:14.63 SS.seek 25ml @16:00:23.01 Vacuum too high -- waiting up to 40 seconds @16:00:23.02 SS.reconfigure :exhale @16:00:23.06 SS.hold @16:00:30.60 Intake @14.2psia, vacuum from 8.7 to 11.7psi @16:00:30.61 SS.reconfigure :inhaling 1.17ml/s @16:00:30.65 SS.seek 25ml @16:00:34.30 Stroke completed -- waiting for pressure to equalize ... @16:00:34.31 SS.reconfigure :exhale @16:00:34.36 SS.hold @16:00:39.98 Intake.select :close @16:00:40.12 SSV.dial :exhaust @16:00:40.88 Exhaust.select :open @16:00:40.99 SS.seek 0ml @16:00:49.86 Exhaust.select :close @16:00:49.97 SSV.dial CBV @16:00:50.02
CBV.dial SSV @16:00:50.74 Allowing 130s for 25.0ml of remaining 925.0ml @16:00:50.75 Intake.select :open @16:00:52.90 Intake @15.0psia, vacuum from 9.5 to 12.5psi @16:00:52.92 SS.reconfigure :inhaling 1.17ml/s @16:00:52.96 SS.seek 25ml @16:01:05.64 Vacuum too high -- waiting up to 40 seconds SS.reconfigure :exhale @16:01:05.68 SS.hold @16:01:12.20 Intake @14.2psia, vacuum from 8.7 to 11.7psi @16:01:12.22 SS.reconfigure :inhaling 1.06ml/s @16:01:12.26 SS.seek 25ml @16:01:23.66 Vacuum too high -- waiting up to 40 seconds @16:01:23.67 SS.reconfigure :exhale @16:01:23.72 SS.hold @16:01:33.46 Intake @14.4psia, vacuum from 8.9 to 11.9psi @16:01:33.47 SS.reconfigure :inhaling 0.95ml/s @16:01:33.52 SS.seek 25ml @16:01:35.42 Stroke completed -- waiting for pressure to equalize ... @16:01:35.43 SS.reconfigure :exhale @16:01:35.47 SS.hold @16:01:40.25 Intake.select :close @16:01:40.38 SSV.dial :exhaust @16:01:41.14 Exhaust.select :open @16:01:41.25 SS.seek 0ml @16:01:49.84 Exhaust.select :close @16:01:49.96 SSV.dial CBV @16:01:49.98
CBV.dial SSV @16:01:50.75 Allowing 130s for 25.0ml of remaining 900.0ml @16:01:50.76 Intake.select :open @16:01:52.91 Intake @15.0psia, vacuum from 9.5 to 12.5psi @16:01:52.93 SS.reconfigure :inhaling 0.95ml/s @16:01:52.97 SS.seek 25ml @16:02:07.35 Vacuum too high -- waiting up to 40 seconds @16:02:07.36 SS.reconfigure :exhale @16:02:07.40 SS.hold @16:02:13.89 Intake @14.1psia, vacuum from 8.6 to 11.6psi @16:02:13.90 SS.reconfigure :inhaling 0.87ml/s @16:02:13.94 SS.seek 25ml @16:02:26.54 Vacuum too high -- waiting up to 40 seconds @16:02:26.55 SS.reconfigure :exhale @16:02:26.59 SS.hold @16:02:36.34 Intake @14.3psia, vacuum from 8.8 to 11.8psi @16:02:36.35 SS.reconfigure :inhaling 0.78ml/s @16:02:36.40 SS.seek 25ml @16:02:40.52 Stroke completed -- waiting for pressure to equalize ... @16:02:40.53 SS.reconfigure :exhale @16:02:40.57 SS.hold @16:02:49.11 Intake.select :close @16:02:49.25 SSV.dial :exhaust @16:02:50.00 Exhaust.select :open @16:02:50.11 SS.seek 0ml @16:02:59.09 Exhaust.select :close @16:02:59.20 SSV.dial CBV @16:02:59.25
CBV.dial SSV @16:02:59.97 Allowing 130s for 25.0ml of remaining 875.0ml @16:02:59.98 Intake.select :open @16:03:02.14 Intake @15.0psia, vacuum from 9.5 to 12.5psi @16:03:02.16 SS.reconfigure :inhaling 0.78ml/s @16:03:02.20 SS.seek 25ml @16:03:18.79 Vacuum too high -- waiting up to 40 seconds @16:03:18.80 SS.reconfigure :exhale @16:03:18.84 SS.hold @16:03:26.31 Intake @14.4psia, vacuum from 8.9 to 11.9psi @16:03:26.33 SS.reconfigure :inhaling 0.70ml/s @16:03:26.37 SS.seek 25ml @16:03:40.38 Vacuum too high -- waiting up to 40 seconds @16:03:40.39 SS.reconfigure :exhale @16:03:40.43 SS.hold @16:03:51.58 Intake @14.5psia, vacuum from 9.0 to 12.0psi @16:03:51.59 SS.reconfigure :inhaling 0.63ml/s @16:03:51.64 SS.seek 25ml @16:03:58.55 Stroke completed -- waiting for pressure to equalize ... @16:03:58.56 SS.reconfigure :exhale @16:03:58.60 SS.hold @16:04:09.63 Intake.select :close @16:04:09.76 SSV.dial :exhaust @16:04:10.52 Exhaust.select :open @16:04:10.62 SS.seek 0ml @16:04:19.37 Exhaust.select :close @16:04:19.48 SSV.dial CBV @16:04:19.53
CBV.dial SSV @16:04:20.25 Allowing 130s for 25.0ml of remaining 850.0ml @16:04:20.26 Intake.select :open @16:04:22.41 Intake @14.9psia, vacuum from 9.4 to 12.4psi @16:04:22.43 SS.reconfigure :inhaling 0.63ml/s @16:04:22.47 SS.seek 25ml @16:04:43.22 Vacuum too high -- waiting up to 40 seconds @16:04:43.23 SS.reconfigure :exhale @16:04:43.28 SS.hold @16:04:53.03 Intake @14.5psia, vacuum from 9.0 to 12.0psi @16:04:53.04 SS.reconfigure :inhaling 0.57ml/s @16:04:53.09 SS.seek 25ml @16:05:12.60 Vacuum too high -- waiting up to 40 seconds @16:05:12.61 SS.reconfigure :exhale @16:05:13.04 SS.hold @16:05:25.80 Intake @14.6psia, vacuum from 9.1 to 12.1psi @16:05:25.81 SS.reconfigure :inhaling 0.51ml/s @16:05:25.86 SS.seek 25ml @16:05:31.23 Stroke completed -- waiting for pressure to equalize ... @16:05:31.24 SS.reconfigure :exhale @16:05:31.28 SS.hold @16:05:42.31 Intake.select :close @16:05:42.45 SSV.dial :exhaust @16:05:43.20 Exhaust.select :open @16:05:43.31 SS.seek 0ml @16:05:52.31 Exhaust.select :close @16:05:52.41 SSV.dial CBV @16:05:52.43
CBV.dial SSV @16:05:53.20 Allowing 130s for 25.0ml of remaining 825.0ml @16:05:53.21 Intake.select :open @16:05:55.37 Intake @15.0psia, vacuum from 9.5 to 12.5psi @16:05:55.38 SS.reconfigure :inhaling 0.51ml/s @16:05:55.42 SS.seek 25ml @16:06:23.94 Vacuum too high -- waiting up to 40 seconds @16:06:23.95 SS.reconfigure :exhale @16:06:23.99 SS.hold @16:06:35.01 Intake @14.6psia, vacuum from 9.1 to 12.1psi @16:06:35.03 SS.reconfigure :inhaling 0.47ml/s @16:06:35.07 SS.seek 25ml @16:06:59.79 Stroke completed -- waiting for pressure to equalize ... @16:06:59.80 SS.reconfigure :exhale @16:06:59.84 SS.hold @16:07:26.25 Intake.select :close @16:07:26.39 SSV.dial :exhaust @16:07:27.14 Exhaust.select :open @16:07:27.24 SS.seek 0ml @16:07:36.39 Exhaust.select :close @16:07:36.49 SSV.dial CBV @16:07:36.51
CBV.dial SSV @16:07:37.28 Allowing 130s for 25.0ml of remaining 800.0ml @16:07:37.29 Intake.select :open @16:07:39.44 Intake @15.1psia, vacuum from 9.6 to 12.6psi @16:07:39.46 SS.reconfigure :inhaling 0.47ml/s @16:07:39.50 SS.seek 25ml @16:08:08.35 Vacuum too high -- waiting up to 40 seconds @16:08:08.36 SS.reconfigure :exhale @16:08:08.40 SS.hold @16:08:21.44 Intake @14.7psia, vacuum from 9.2 to 12.2psi @16:08:21.45 SS.reconfigure :inhaling 0.43ml/s @16:08:21.50 SS.seek 25ml @16:08:48.22 Vacuum too high -- waiting up to 40 seconds @16:08:48.23 SS.reconfigure :exhale @16:08:48.28 SS.hold @16:09:04.45 Intake @14.6psia, vacuum from 9.1 to 12.1psi @16:09:04.46 SS.reconfigure :inhaling 0.38ml/s @16:09:04.51 SS.seek 25ml @16:09:08.26 Stroke completed -- waiting for pressure to equalize ... @16:09:08.27 SS.reconfigure :exhale @16:09:08.31 SS.hold @16:09:24.60 Intake.select :close @16:09:24.74 SSV.dial :exhaust @16:09:25.49 Exhaust.select :open @16:09:25.60 SS.seek 0ml @16:09:34.71 Exhaust.select :close @16:09:34.81 SSV.dial CBV @16:09:34.84
CBV.dial SSV @16:09:35.61 Allowing 130s for 25.0ml of remaining 775.0ml @16:09:35.62 Intake.select :open @16:09:37.77 Intake @15.0psia, vacuum from 9.5 to 12.5psi @16:09:37.79 SS.reconfigure :inhaling 0.38ml/s @16:09:37.83 SS.seek 25ml @16:10:17.00 Vacuum too high -- waiting up to 40 seconds @16:10:17.01 SS.reconfigure :exhale @16:10:17.05 SS.hold @16:10:31.30 Intake @14.7psia, vacuum from 9.2 to 12.2psi @16:10:31.32 SS.reconfigure :inhaling 0.34ml/s @16:10:31.36 SS.seek 25ml @16:11:05.32 Stroke completed -- waiting for pressure to equalize ... @16:11:05.33 SS.reconfigure :exhale @16:11:05.37 SS.hold @16:11:35.93 Intake.select :close @16:11:36.06 SSV.dial :exhaust @16:11:36.82 Exhaust.select :open @16:11:36.93 SS.seek 0ml @16:11:45.66 Exhaust.select :close @16:11:45.77 SSV.dial CBV @16:11:46.30
CBV.dial SSV @16:11:47.04 Allowing 130s for 25.0ml of remaining 750.0ml @16:11:47.05 Intake.select :open @16:11:49.20 Intake @14.9psia, vacuum from 9.4 to 12.4psi @16:11:49.22 SS.reconfigure :inhaling 0.34ml/s @16:11:49.26 SS.seek 25ml @16:12:35.69 Vacuum too high -- waiting up to 40 seconds @16:12:35.70 SS.reconfigure :exhale @16:12:35.74 SS.hold @16:12:49.99 Intake @14.6psia, vacuum from 9.1 to 12.1psi @16:12:50.00 SS.reconfigure :inhaling 0.31ml/s @16:12:50.04 SS.seek 25ml @16:13:27.86 Stroke completed -- waiting for pressure to equalize ... @16:13:27.87 SS.reconfigure :exhale @16:13:27.91 SS.hold @16:13:57.06 SS.reconfigure :default @16:13:57.10 SS.hold @16:13:57.20 Intake.select :close @16:13:57.34 ShallowSampler::Clogged in MAIN -- Filtering too Slowly after 275.0ml @16:13:57.35 SSV.dial :exhaust @16:13:58.11 Exhaust.select :open @16:13:58.22 SS.reconfigure :exhale @16:13:58.26 SS.seek 0ml @16:14:07.24 Exhaust.select :close @16:14:07.34 Sampled 275.0ml @16:14:07.35 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @16:14:07.36 Intake.select :close @16:14:07.43 CSV.dial :waste @16:14:07.47 CS.seek 0ml @16:14:08.15 CSV.dial :air @16:14:08.18 SSV.dial CBV,avoiding: :exhaust @16:14:08.21
CBV.dial SSV @16:14:08.96 CS.seek 10ml @16:14:10.10
CTV.dial :air @16:14:10.89 delay 15 seconds @16:14:25.91 SSV.dial :spit,avoiding: :exhaust @16:14:27.23 CTV.dial CSV,avoiding: :intake @16:14:27.27
SS.seek 0ml @16:14:27.38 SSV.dial CBV,avoiding: :exhaust @16:14:33.46 CBV.dial between SSV and waste2 @16:14:33.85 CTV.dial CSV,avoiding: :air @16:14:33.86
CSV.dial CTV @16:14:34.32 CS.reconfigure :pressurize @16:14:34.36 CS.seek 0ml @16:14:52.59 BubblePoint: Intake @38.7psia, Outlet @14.6psia @16:14:52.60 CBV.dial SSV @16:14:52.94 SS.reconfigure :default @16:14:52.98 SS.seek 10ml @16:14:58.43 delay 15 seconds @16:15:13.55 Evacuated: Intake @17.2psia, Outlet @8.2psia @16:15:13.56 SSV.dial :spit,avoiding: :exhaust @16:15:14.89 SS.reconfigure :exhale @16:15:14.93 SS.seek 0ml @16:15:20.09 SS.reconfigure :fast @16:15:20.13 SS.seek 5ml @16:15:23.09 SSV.dial CBV,avoiding: :exhaust @16:15:23.11
CBV.dial SSV @16:15:24.43 CTV.dial :air,avoiding: :intake @16:15:25.78 CSV.dial CTV @16:15:25.81
SS.seek 10ml @16:15:26.82 CSV.dial :air @16:15:27.27 CS.reconfigure :fast @16:15:27.31 CS.seek 0ml @16:15:28.62
SSV.dial :spit,avoiding: :exhaust @16:15:29.93 SS.reconfigure :exhale @16:15:29.97 SS.seek 0ml @16:15:35.12 CS.stop @16:15:35.44
SS.reconfigure :fast @16:15:35.48 SS.seek 5ml @16:15:38.21 SSV.dial CBV,avoiding: :exhaust @16:15:38.23
CBV.dial SSV @16:15:40.56 SSV.dial :spit,avoiding: :exhaust @16:15:41.87 SS.reconfigure :default @16:15:41.91 SS.seek 0ml @16:15:45.06 SS.stop @16:15:45.10 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @16:15:45.11 Intake.select :close @16:15:45.18 CSV.dial :waste @16:15:45.30 SSV.dial CBV,avoiding: :exhaust @16:15:45.32
CBV.dial SSV @16:15:45.98 CS.seek 0ml @16:15:46.04 CSV.dial :air @16:15:46.65
CTV.dial :air @16:15:46.66 delay 15 seconds @16:15:46.77 CS.seek 10ml @16:16:01.68
SSV.dial :spit,avoiding: :exhaust @16:16:03.02 CTV.dial CSV,avoiding: :intake @16:16:03.07
SS.reconfigure :exhale @16:16:03.13 SS.seek 0ml @16:16:03.57 SSV.dial CBV,avoiding: :exhaust @16:16:11.12 CBV.dial between SSV and waste2 @16:16:11.52 CTV.dial CSV,avoiding: :air @16:16:11.54
CSV.dial CTV @16:16:12.01 CS.reconfigure :pressurize @16:16:12.05 CS.seek 0ml @16:16:33.41 BubblePoint: Intake @37.7psia, Outlet @13.5psia CBV.dial SSV @16:16:33.74 SS.reconfigure :default @16:16:33.78 SS.seek 10ml @16:16:39.30 delay 15 seconds @16:16:54.42 Evacuated: Intake @18.5psia, Outlet @10.8psia @16:16:54.43 SSV.dial :spit,avoiding: :exhaust @16:16:55.74 SS.reconfigure :exhale @16:16:55.79 SS.seek 0ml @16:17:01.01 SS.reconfigure :fast @16:17:01.05 SS.seek 5ml @16:17:04.03 SSV.dial CBV,avoiding: :exhaust @16:17:04.05
CBV.dial SSV @16:17:05.38 CTV.dial :air,avoiding: :intake @16:17:06.73 CSV.dial CTV @16:17:06.77
SS.seek 10ml @16:17:07.77 CSV.dial :air @16:17:08.24 CS.reconfigure :fast @16:17:08.28 CS.seek 0ml @16:17:09.35
SSV.dial :spit,avoiding: :exhaust @16:17:10.67 SS.reconfigure :exhale @16:17:10.71 SS.seek 0ml @16:17:13.34 CS.stop @16:17:15.68
SS.reconfigure :fast @16:17:15.73 SS.seek 5ml @16:17:18.54 SSV.dial CBV,avoiding: :exhaust @16:17:18.56
CBV.dial SSV @16:17:20.90 SSV.dial :spit,avoiding: :exhaust @16:17:22.21 SS.reconfigure :default @16:17:22.25 SS.seek 0ml @16:17:25.50 SS.stop @16:17:25.54 Lysing: Adding 2.0ml AqMeOH @16:17:25.55 CBV.dial between CSV and waste1,avoiding: :DBV @16:17:27.06 CTV.dial CSV @16:17:28.39 CSV.dial CSR @16:17:29.17 Pulling 1ml of CSR.AqMeOH @16:17:29.18 CSR.select :AqMeOH @16:17:29.29 CS.reconfigure :default @16:17:29.33 CS.seek 1ml @16:17:32.72 delay 5 seconds @16:17:37.73 CSR.select :air @16:17:37.89 CS.seek 6ml @16:17:51.53 delay 2 seconds @16:17:53.54 CSV.dial :waste2 @16:17:54.11 CS.reconfigure :fast @16:17:54.15 CS.seek 0ml @16:18:08.68 CSV.dial CSR @16:18:09.16 Pulling 2ml of CSR.AqMeOH @16:18:09.17 CSR.select :AqMeOH @16:18:09.27 CS.reconfigure :default @16:18:09.31 CS.seek 2ml @16:18:15.31 delay 5 seconds @16:18:20.33 CSR.select :air @16:18:20.48 CS.seek 5ml @16:18:29.12 delay 2 seconds @16:18:31.14 CSV.dial CTV @16:18:31.70 CS.seek 1ml @16:18:42.85 Before lysing Collection Puck Heater at 12.2C/37.7psia @16:18:42.87 Heating Puck.from 3, :sh1 in Collection Clamp to 70C for 10:00 @16:18:42.88 CH.seek 70C,8:00 @16:20:45.87 CH.hold 10:00,70C @16:20:46.04 Begun lysing Collection Puck Heater at 68.1C/45.4psia @16:30:46.06 Ended lysing Collection Puck Heater at 70C/44.8psia @16:30:46.08 CS.seek 0ml @16:30:49.55 CTV.dial between spare and air,avoiding: :intake @16:30:50.60 CSV.dial :air @16:30:51.07 CS.seek 5ml @16:31:04.86 CSV.dial CTV @16:31:05.30 delay 2 seconds @16:31:07.31 CSV.dial :air @16:31:07.77 delay 2 seconds @16:31:09.78 CSV.dial CTV @16:31:10.24 delay 2 seconds @16:31:12.25 CSV.dial :air @16:31:12.71 delay 2 seconds @16:31:14.73 CS.seek 0ml @16:31:28.38 Puck @72.6psia after final lysis pressurization @16:31:28.40 Recovering Lysate into Collection syringe CSV.dial CBV @16:31:29.41 CBV.dial CSV @16:31:29.76 CS.seek 5ml @16:31:43.55 delay 5 seconds @16:31:48.57 CTV.dial :air @16:31:49.09 CS.seek 7ml @16:31:54.95 delay 5 seconds @16:31:59.97 CSV.dial :air2 @16:32:00.41 CS.seek 10ml @16:32:08.86 CSV.dial between air and CTV,avoiding: CBV @16:32:10.02 Lysing Complete @16:32:10.09 Storing sh1 puck in Collection Clamp to tube 2 @16:32:10.25 SC.reconfigure :tight @16:32:10.29 SC.seek 2 @16:32:13.85 SC.configure :limp @16:32:14.04 Elbow.reconfigure :tight @16:32:14.09 Elbow.seek 2 @16:32:15.86 Forearm.reconfigure :tight @16:32:15.90 Forearm.seek 2 + 450 counts @16:32:16.91 SE.reconfigure :pick @16:32:16.96 SE.seek :beamBreak @16:32:19.39 SE.configure :default @16:32:19.53 Elbow.reconfigure :limp @16:32:19.57 Forearm.reconfigure :loose @16:32:19.61 Forearm.seek 2 @16:32:19.87 Elbow.hold @16:32:19.93 Elbow.reconfigure :tight @16:32:20.53 SE.seek 10.11 Pucks below Top Plate @16:32:20.97 Forearm.force -20 @16:32:21.01 Forearm.seek 2 + 267 counts @16:32:21.28 Forearm.reconfigure :tight @16:32:21.33 Forearm.seek 2 + 450 counts @16:32:21.52 SE.seek 11.11 Pucks below Top Plate @16:32:22.03 Forearm.reconfigure :loose @16:32:22.08 Forearm.seek :retract @16:32:23.05 Elbow.seek CC @16:32:24.77 Elbow.reconfigure :loose @16:32:24.86 Forearm.seek CC @16:32:25.81 Hand.close @16:32:26.39 CC.seek :open @16:32:31.10 Forearm.seek :retract @16:32:32.04 Elbow.reconfigure :tight @16:32:32.09 Elbow.seek 2 @16:32:33.87 Elbow.reconfigure :limp @16:32:33.91 Forearm.seek 2 @16:32:34.89 Elbow.hold @16:32:34.95 Elbow.reconfigure :tight @16:32:34.99 Hand.open @16:32:35.75 Forearm.force -20 @16:32:35.78 Forearm.seek 2 + 305 counts @16:32:36.16 Forearm.reconfigure :tight @16:32:36.21 Forearm.seek 2 + 450 counts @16:32:36.48 SE.seek :down @16:32:37.60
Forearm.reconfigure :loose @16:32:37.64 Forearm.seek :retract @16:32:38.44 SE.stop @16:32:38.61
Tranferring DA @16:32:38.62 Beginning samplePassC @16:32:38.63 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @16:32:38.81 Elbow.seek FlushPuck::Garage @16:32:40.74 Elbow.reconfigure :loose @16:32:40.83 Forearm.seek :garage @16:32:41.81 Hand.close @16:32:42.50 Forearm.seek :retract @16:32:43.47 Elbow.reconfigure :tight @16:32:43.51 Elbow.seek CC @16:32:46.61 Elbow.reconfigure :loose @16:32:46.70 Forearm.reconfigure :tight @16:32:46.74 Forearm.seek CC @16:32:47.94 CC.seek :closed @16:32:52.44 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.30 @16:32:52.51 Hand.open @16:32:53.20 Forearm.reconfigure :loose @16:32:53.24 Forearm.seek :retract @16:32:54.18 PSV.dial PSR @16:32:55.58 Pulling 1.5ml of PSR.flush @16:32:55.59 PSR.select :flush @16:32:55.70 PS.seek 1.5ml @16:33:11.84 delay 5 seconds @16:33:16.86 PSV.dial :waste1 @16:33:17.31 PS.seek 0ml @16:33:33.40 PSV.dial PSR @16:33:33.85 PSR.select :air @16:33:33.96 PS.seek 2.5ml @16:34:00.25 PRV.dial CBV,avoiding: :ab2 @16:34:00.29
CBV.dial PRV @16:34:01.95 PSV.dial :mixloop @16:34:03.00 CTV.dial :air,avoiding: :intake @16:34:03.01 PTV.dial :PRVmixing @16:34:04.34 PS.seek 0ml @16:34:31.15 PSV.dial :air @16:34:31.61 PS.seek 2.5ml @16:34:57.99 PSV.dial :mixloop @16:34:58.58 PS.seek 0ml @16:35:24.96 PSV.dial :air @16:35:25.53 PS.seek 2.5ml @16:35:51.80 PSV.dial :mixloop @16:35:52.27 PS.seek 0ml @16:36:18.56 Garaging FlushPuck in Collection Clamp @16:36:18.63 Elbow.reconfigure :tight @16:36:18.67 Elbow.seek CC @16:36:19.08 Elbow.reconfigure :loose @16:36:19.17 Forearm.seek CC @16:36:20.19 Hand.close @16:36:20.76 CC.seek :open @16:36:25.37 Forearm.seek :retract @16:36:26.36 Elbow.reconfigure :tight @16:36:26.40 Elbow.seek :garage @16:36:29.36 Elbow.reconfigure :loose @16:36:29.45 Forearm.reconfigure :tight @16:36:29.49 Forearm.seek :garage @16:36:30.52 Hand.open @16:36:31.19 Forearm.reconfigure :loose @16:36:31.23 Forearm.seek :retract @16:36:32.20 PSV.dial :mixloop @16:36:32.21 PTV.dial :PRVmixing @16:36:32.22 PRV.dial CBV @16:36:32.24
CBV.dial PRV @16:36:32.26 CTV.dial :air,avoiding: :intake @16:36:32.28 Loading lyfil puck from tube 3 into Collection Clamp @16:36:32.50 SC.reconfigure :tight @16:36:32.54 SC.seek 3 @16:36:36.15 SC.configure :limp @16:36:36.35 Elbow.reconfigure :tight @16:36:36.39 Elbow.seek 3 @16:36:38.36 Forearm.reconfigure :tight @16:36:38.40 Forearm.seek 3 + 450 counts @16:36:39.55 SE.reconfigure :pick @16:36:39.60 SE.seek :beamBreak @16:36:41.36 SE.configure :default @16:36:41.50 Elbow.seek 3 @16:36:41.66 Forearm.seek 3 + 300 counts @16:36:41.82 Forearm.reconfigure :loose @16:36:41.86 Hand.close @16:36:42.52 SE.seek :down @16:36:42.71
Forearm.seek :retract @16:36:43.67 Elbow.seek CC @16:36:44.00 SE.stop @16:36:45.33
Elbow.reconfigure :loose @16:36:45.42 Forearm.reconfigure :tight @16:36:45.46 Forearm.seek CC @16:36:46.75 CC.seek :closed @16:36:51.26 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.20 @16:36:51.27 Hand.open @16:36:51.95 Forearm.reconfigure :loose @16:36:51.99 Forearm.seek :retract @16:36:52.93 CTV.dial CSV,avoiding: :intake @16:36:54.31 CSV.dial CTV @16:36:54.64 PS.seek 2.5ml @16:37:21.11 CS.seek 4.5ml @16:37:35.95 delay 8 seconds @16:37:43.97 PSV.dial :air @16:37:44.55 delay 2 seconds @16:37:46.57 PSV.dial :waste2 @16:37:47.81 PS.seek 0ml @16:38:14.08 CBV.dial between PRV and waste1 @16:38:14.40 CSV.dial :air @16:38:14.88 CS.seek 10ml @16:38:29.73 CSV.dial CTV @16:38:30.17 CS.seek 0ml @16:38:56.51 CBV.dial :waste1 @16:38:56.82 delay 5 seconds @16:39:01.85 CTV.dial :air,avoiding: :intake @16:39:03.27 Storing lyfil puck in Collection Clamp to tube 2 @16:39:03.44 SC.reconfigure :tight @16:39:03.48 SC.seek 2 @16:39:07.04 SC.configure :limp @16:39:07.23 Elbow.reconfigure :tight @16:39:07.27 Elbow.seek 2 @16:39:09.06 Forearm.reconfigure :tight @16:39:09.10 Forearm.seek 2 + 450 counts @16:39:10.13 SE.reconfigure :pick @16:39:10.18 SE.seek :beamBreak @16:39:12.55 SE.configure :default @16:39:12.69 Elbow.reconfigure :limp @16:39:12.74 Forearm.reconfigure :loose @16:39:12.78 Forearm.seek 2 @16:39:13.05 Elbow.hold @16:39:13.11 Elbow.reconfigure :tight @16:39:13.20 SE.seek 11.1 Pucks below Top Plate @16:39:13.64 Forearm.force -20 @16:39:13.67 Forearm.seek 2 + 286 counts @16:39:13.95 Forearm.reconfigure :tight @16:39:13.99 Forearm.seek 2 + 450 counts @16:39:14.30 SE.seek 12.1 Pucks below Top Plate @16:39:14.81 Forearm.reconfigure :loose @16:39:14.85 Forearm.seek :retract @16:39:15.74 Elbow.seek CC @16:39:17.50 Elbow.reconfigure :loose @16:39:17.60 Forearm.seek CC @16:39:18.54 Hand.close @16:39:19.13 CC.seek :open @16:39:23.84 Forearm.seek :retract @16:39:24.87 Elbow.reconfigure :tight @16:39:24.91 Elbow.seek 2 @16:39:26.67 Elbow.reconfigure :limp @16:39:26.71 Forearm.seek 2 @16:39:27.66 Elbow.hold @16:39:27.72 Elbow.reconfigure :tight @16:39:27.76 Hand.open @16:39:28.50 Forearm.force -20 @16:39:28.54 Forearm.seek 2 + 306 counts @16:39:28.82 Forearm.reconfigure :tight @16:39:28.86 Forearm.seek 2 + 450 counts @16:39:29.10 SE.seek :down @16:39:30.23
Forearm.reconfigure :loose @16:39:30.27 Forearm.seek :retract @16:39:31.06 SE.stop @16:39:31.16
PSV.dial :air @16:39:31.92 PS.seek 2.5ml @16:39:58.33 delay 1 second @16:39:59.35 PSV.dial :mixloop @16:39:59.79 delay 1 second @16:40:00.80 PSV.dial :air @16:40:01.25 delay 1 second @16:40:02.27 PSV.dial :mixloop @16:40:02.72 delay 1 second @16:40:03.74 PSV.dial :air @16:40:04.19 delay 1 second @16:40:05.21 PS.seek 0ml @16:40:31.52 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @16:40:31.69 Elbow.seek FlushPuck::Garage @16:40:33.89 Elbow.reconfigure :loose @16:40:34.05 Forearm.seek :garage @16:40:35.05 Hand.close @16:40:35.74 Forearm.seek :retract @16:40:36.73 Elbow.reconfigure :tight @16:40:36.77 Elbow.seek PC @16:40:38.08 Elbow.reconfigure :loose @16:40:38.17 Forearm.reconfigure :tight @16:40:38.21 Forearm.seek PC @16:40:39.39 PC.seek :closed @16:40:44.03 Processing Clamp onSpring/offSpring for 16/26 samples, on/off torque 1.58 @16:40:44.10 Hand.open @16:40:44.81 Forearm.reconfigure :loose @16:40:44.86 Forearm.seek :retract @16:40:45.79 PRV.dial :air @16:40:46.25 PSV.dial :mixloop @16:40:46.79 PS.seek 0.3ml @16:40:50.64 PSV.dial PSR @16:40:51.74 Pulling 0.4ml of PSR.air @16:40:51.75 PSR.select :air @16:40:51.78 PS.seek 0.7ml @16:40:56.68 delay 3 seconds @16:40:59.70 PSR.select :air @16:40:59.72 PS.seek 2.5ml @16:41:18.92 delay 3 seconds @16:41:21.94 PSV.dial :mixloop @16:41:23.00 PTV.dial :mixing @16:41:23.77 PBV.dial :waste2 @16:41:24.71 PS.seek 0ml @16:41:51.08 Garaging FlushPuck in Processing Clamp @16:41:51.15 Elbow.reconfigure :tight @16:41:51.19 Elbow.seek PC @16:41:51.29 Elbow.reconfigure :loose @16:41:51.38 Forearm.seek PC @16:41:52.35 Hand.close @16:41:53.01 PC.seek :open @16:41:57.72 Forearm.seek :retract @16:41:58.66 Elbow.reconfigure :tight @16:41:58.71 Elbow.seek :garage @16:42:00.06 Elbow.reconfigure :loose @16:42:00.15 Forearm.reconfigure :tight @16:42:00.19 Forearm.seek :garage @16:42:01.13 Hand.open @16:42:01.81 Forearm.reconfigure :loose @16:42:01.85 Forearm.seek :retract @16:42:02.85 Loading da puck from tube 3 into Processing Clamp @16:42:03.06 SC.reconfigure :tight @16:42:03.10 SC.seek 3 @16:42:06.64 SC.configure :limp @16:42:06.83 Elbow.reconfigure :tight @16:42:06.87 Elbow.seek 3 @16:42:08.77 Forearm.reconfigure :tight @16:42:08.81 Forearm.seek 3 + 450 counts @16:42:09.85 SE.reconfigure :pick @16:42:09.90 SE.seek :beamBreak @16:42:11.81 SE.configure :default @16:42:11.96 Elbow.seek 3 @16:42:12.12 Forearm.seek 3 + 300 counts @16:42:12.30 Forearm.reconfigure :loose @16:42:12.34 Hand.close @16:42:13.02 SE.seek :down @16:42:13.20
Forearm.seek :retract @16:42:14.15 Elbow.seek PC @16:42:14.51 SE.stop @16:42:16.81
Elbow.reconfigure :loose @16:42:16.90 Forearm.reconfigure :tight @16:42:16.94 Forearm.seek PC @16:42:18.64 PC.seek :closed @16:42:23.27 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.60 @16:42:23.28 Hand.open @16:42:23.94 Forearm.reconfigure :loose @16:42:23.98 Forearm.seek :retract @16:42:24.95 Heating Puck.from 3, :da in Processing Clamp to 28C for 2:00:00 @16:42:24.97 PH.seek 28C,5:00 @16:43:07.99 PH.hold 2:00:00,28C @16:43:08.04 delay 1:30 @16:44:38.08 PSR.select :air @16:44:38.10 PSV.dial PSR @16:44:39.21 Pulling 0.5ml of PSR.wash @16:44:39.22 PSR.select :wash @16:44:39.34 PS.seek 0.5ml @16:44:45.21 delay 5 seconds @16:44:50.22 PSR.select :air @16:44:50.34 PS.seek 2ml @16:45:06.51 PTV.dial :puck @16:45:07.25 PSV.dial PTV @16:45:08.01 PBV.dial :waste1 @16:45:09.44 PS.seek 1.5ml @16:45:15.36 delay 3 seconds @16:45:18.38 PBV.dial between PSV and waste1 @16:45:18.74 PS.seek 0ml @16:45:34.86 delay 5:00 @16:50:34.88 recoverPSRreagent08 @16:50:34.89 PTV.dial :puck @16:50:34.90 PBV.dial :waste1 @16:50:35.26 delay 30 seconds @16:51:05.28 PBV.dial between PSV and waste1 @16:51:05.65 PSR.select :air @16:51:05.67 PSV.dial PSR @16:51:06.44 PS.reconfigure :fast @16:51:06.48 PS.seek 2.5ml @16:51:30.23 PSV.dial PTV @16:51:30.99 delay 5 seconds @16:51:36.01 PSV.dial PSR @16:51:36.78 delay 5 seconds @16:51:41.79 PSV.dial PTV @16:51:42.56 delay 5 seconds @16:51:47.57 PSV.dial :air @16:51:48.34 PS.seek 0ml @16:52:11.51 PTV.dial :PRVmixing,via: PRV @16:52:12.85 PSV.dial :mixloop @16:52:13.32 PRV.dial :ab1 @16:52:14.08 PS.reconfigure :slow @16:52:14.12 PS.seek 0.15ml @16:52:17.73 delay 2 seconds @16:52:19.74 CBV.dial PRV @16:52:19.77
PRV.dial CBV @16:52:20.35 PS.seek 0.35ml @16:52:24.95 delay 2 seconds @16:52:26.96 CBV.dial between PRV and PSV @16:52:27.27 PRV.dial :air @16:52:27.72 PS.seek 0.9ml @16:52:39.44 PRV.dial :waste2 @16:52:40.19 PS.seek 0.5ml @16:52:48.85 addPMLReagent08 @16:52:48.86 PSV.dial :air @16:52:49.33 PS.reconfigure :default @16:52:49.37 PS.seek 2ml @16:53:05.51 PSV.dial :PML @16:53:05.96 PTV.dial :mixing @16:53:06.69 PBV.dial :waste1 @16:53:07.11 PS.seek 1.85ml @16:53:09.44 PBV.dial between PSV and waste1 @16:53:09.83 PS.seek 0ml @16:53:30.07 delay 2 seconds @16:53:32.13 Beginning samplePassC @16:53:32.15 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @16:53:32.31 Elbow.reconfigure :tight @16:53:32.35 Elbow.seek FlushPuck::Garage @16:53:33.73 Elbow.reconfigure :loose @16:53:33.81 Forearm.seek :garage @16:53:34.81 Hand.close @16:53:35.49 Forearm.seek :retract @16:53:36.47 Elbow.reconfigure :tight @16:53:36.51 Elbow.seek CC @16:53:39.51 Elbow.reconfigure :loose @16:53:39.60 Forearm.reconfigure :tight @16:53:39.64 Forearm.seek CC @16:53:40.91 CC.seek :closed @16:53:45.43 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.43 @16:53:45.50 Hand.open @16:53:46.18 Forearm.reconfigure :loose @16:53:46.22 Forearm.seek :retract @16:53:47.20 PSV.dial PSR @16:53:48.31 Pulling 1.5ml of PSR.flush @16:53:48.32 PSR.select :flush @16:53:48.44 PS.seek 1.5ml @16:54:04.54 delay 5 seconds @16:54:09.56 PSV.dial :waste1 @16:54:10.03 PS.seek 0ml @16:54:26.38 PSV.dial PSR @16:54:26.85 PSR.select :air @16:54:26.96 PS.seek 2.5ml @16:54:53.26 PRV.dial CBV,avoiding: :ab2 @16:54:53.28
CBV.dial PRV @16:54:54.91 PSV.dial :mixloop @16:54:55.96 CTV.dial :air,avoiding: :intake @16:54:55.97 PTV.dial :PRVmixing @16:54:56.74 PS.seek 0ml @16:55:23.13 PSV.dial :air @16:55:23.59 PS.seek 2.5ml @16:55:50.03 PSV.dial :mixloop @16:55:50.49 PS.seek 0ml @16:56:16.88 PSV.dial :air @16:56:17.48 PS.seek 2.5ml @16:56:43.81 PSV.dial :mixloop @16:56:44.26 PS.seek 0ml @16:57:10.63 Garaging FlushPuck in Collection Clamp @16:57:10.70 Elbow.reconfigure :tight @16:57:10.74 Elbow.seek CC @16:57:11.17 Elbow.reconfigure :loose @16:57:11.28 Forearm.seek CC @16:57:12.24 Hand.close @16:57:12.87 CC.seek :open @16:57:17.51 Forearm.seek :retract @16:57:18.51 Elbow.reconfigure :tight @16:57:18.56 Elbow.seek :garage @16:57:21.56 Elbow.reconfigure :loose @16:57:21.66 Forearm.reconfigure :tight @16:57:21.70 Forearm.seek :garage @16:57:22.74 Hand.open @16:57:23.43 Forearm.reconfigure :loose @16:57:23.47 Forearm.seek :retract @16:57:24.50 delayUntil "17:01:32.12" @16:57:24.52 Whole Cell Archive sampling 1000ml @16:57:24.54 Loading wcr puck from tube 3 into Collection Clamp @16:57:25.88 Elbow.reconfigure :tight @16:57:25.93 Elbow.seek 3 @16:57:28.36 Forearm.reconfigure :tight @16:57:28.42 Forearm.seek 3 + 450 counts @16:57:29.43 SE.reconfigure :pick @16:57:29.49 SE.seek :beamBreak @16:57:31.40 SE.configure :default @16:57:31.56 Elbow.seek 3 @16:57:31.73 Forearm.seek 3 + 300 counts @16:57:31.93 Forearm.reconfigure :loose @16:57:31.98 Hand.close @16:57:32.63 SE.seek :down @16:57:32.82 Forearm.seek :retract @16:57:33.78 Elbow.seek CC @16:57:34.21 SE.stop @16:57:35.44 Elbow.reconfigure :loose @16:57:35.54 Forearm.reconfigure :tight @16:57:35.58 Forearm.seek CC @16:57:36.79 CC.seek :closed @16:57:41.31 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.23 @16:57:41.32 Hand.open @16:57:41.93 Forearm.reconfigure :loose @16:57:41.97 Forearm.seek :retract @16:57:42.95 collectSample 1000ml w/bubble point of 35psi @16:57:42.97 Priming 7ml @16:57:42.98 Intake.select :close @16:57:42.99 Exhaust.select :close @16:57:43.00 SS.hold @16:57:43.20 Calibrating pressure sensors @16:57:43.21 CTV.dial :air @16:57:43.22 SSV.dial :spit,avoiding: :exhaust @16:57:43.24 SS.reconfigure :fast @16:57:43.28 SS.seek 5ml @16:57:46.07 SSV.dial CBV @16:57:46.10 CBV.dial SSV @16:57:47.43 delay 3.8 seconds @16:57:51.35 SSV.dial :spit @16:57:52.67 SS.seek 0ml @16:57:55.46 SSV.dial CBV @16:57:56.79 CTV.dial :intake @16:57:57.56 SSV.dial CBV @16:57:57.58 CBV.dial SSV @16:57:57.60 Allowing 40s for 7.0ml of remaining 7.0ml @16:57:57.61 Intake.select :open @16:57:59.77 Intake @14.8psia, vacuum from 9.3 to 12.3psi @16:57:59.79 SS.reconfigure :inhaling 2.20ml/s @16:57:59.83 SS.seek 7ml @16:58:05.21 Stroke completed -- waiting for pressure to equalize ... @16:58:05.22 SS.reconfigure :exhale @16:58:05.26 SS.hold @16:58:16.30 Intake.select :close @16:58:16.44 SSV.dial :exhaust @16:58:17.18 Exhaust.select :open @16:58:17.29 SS.seek 0ml @16:58:21.11 Exhaust.select :close @16:58:21.22 Sampling 1000.0ml @16:58:21.24 Intake.select :close @16:58:21.25 Exhaust.select :close @16:58:21.26 SS.reconfigure :default @16:58:21.30 SS.hold @16:58:21.97 CTV.dial :intake @16:58:21.99 SSV.dial CBV @16:58:22.01 CBV.dial SSV @16:58:22.79 Allowing 130s for 25.0ml of remaining 1000.0ml @16:58:22.80 Intake.select :open @16:58:24.96 Intake @15.6psia, vacuum from 10.0 to 13.0psi @16:58:24.98 SS.reconfigure :inhaling 2.20ml/s @16:58:25.02 SS.seek 25ml @16:58:32.45 Vacuum too high -- waiting up to 40 seconds @16:58:32.46 SS.reconfigure :exhale @16:58:32.51 SS.hold @16:58:35.85 Intake @13.6psia, vacuum from 8.1 to 11.1psi @16:58:35.87 SS.reconfigure :inhaling 1.98ml/s @16:58:35.91 SS.seek 25ml @16:58:41.14 Vacuum too high -- waiting up to 40 seconds @16:58:41.15 SS.reconfigure :exhale @16:58:41.19 SS.hold @16:58:47.06 Intake @14.0psia, vacuum from 8.5 to 11.5psi @16:58:47.08 SS.reconfigure :inhaling 1.79ml/s @16:58:47.12 SS.seek 25ml @16:58:52.35 Stroke completed -- waiting for pressure to equalize ... @16:58:52.36 SS.reconfigure :exhale @16:58:52.40 SS.hold @16:58:58.82 Intake.select :close @16:58:58.96 SSV.dial :exhaust @16:58:59.72 Exhaust.select :open @16:58:59.83 SS.seek 0ml @16:59:08.57 Exhaust.select :close @16:59:08.67 SSV.dial CBV @16:59:08.70 CBV.dial SSV @16:59:09.46 Allowing 130s for 25.0ml of remaining 975.0ml @16:59:09.47 Intake.select :open @16:59:11.63 Intake @15.4psia, vacuum from 9.9 to 12.9psi @16:59:11.65 SS.reconfigure :inhaling 1.79ml/s @16:59:11.69 SS.seek 25ml @16:59:22.20 Vacuum too high -- waiting up to 40 seconds @16:59:22.22 SS.reconfigure :exhale @16:59:22.26 SS.hold @16:59:26.00 Intake @13.4psia, vacuum from 7.9 to 10.9psi @16:59:26.02 SS.reconfigure :inhaling 1.61ml/s @16:59:26.06 SS.seek 25ml @16:59:34.11 Stroke completed -- waiting for pressure to equalize ... @16:59:34.12 SS.reconfigure :exhale @16:59:34.17 SS.hold @16:59:43.85 Intake.select :close @16:59:43.99 SSV.dial :exhaust @16:59:45.25 Exhaust.select :open @16:59:45.36 SS.seek 0ml @16:59:54.10 Exhaust.select :close @16:59:54.20 SSV.dial CBV @16:59:54.22 CBV.dial SSV @16:59:55.01 Allowing 130s for 25.0ml of remaining 950.0ml @16:59:55.02 Intake.select :open @16:59:57.17 Intake @15.3psia, vacuum from 9.8 to 12.8psi @16:59:57.19 SS.reconfigure :inhaling 1.61ml/s @16:59:57.24 SS.seek 25ml @17:00:08.76 Vacuum too high -- waiting up to 40 seconds @17:00:08.77 SS.reconfigure :exhale @17:00:08.82 SS.hold @17:00:12.54 Intake @13.4psia, vacuum from 7.9 to 10.9psi @17:00:12.56 SS.reconfigure :inhaling 1.45ml/s @17:00:12.60 SS.seek 25ml @17:00:21.03 Stroke completed -- waiting for pressure to equalize ... @17:00:21.04 SS.reconfigure :exhale @17:00:21.09 SS.hold @17:00:30.76 Intake.select :close @17:00:30.90 SSV.dial :exhaust @17:00:31.67 Exhaust.select :open @17:00:31.78 SS.seek 0ml @17:00:40.50 Exhaust.select :close @17:00:40.61 SSV.dial CBV @17:00:40.63 CBV.dial SSV @17:00:41.41 Allowing 130s for 25.0ml of remaining 925.0ml @17:00:41.42 Intake.select :open @17:00:43.58 Intake @15.3psia, vacuum from 9.8 to 12.8psi @17:00:43.60 SS.reconfigure :inhaling 1.45ml/s @17:00:43.64 SS.seek 25ml @17:00:55.95 Vacuum too high -- waiting up to 40 seconds @17:00:55.96 SS.reconfigure :exhale @17:00:56.00 SS.hold @17:01:00.27 Intake @13.6psia, vacuum from 8.1 to 11.1psi @17:01:00.29 SS.reconfigure :inhaling 1.30ml/s @17:01:00.33 SS.seek 25ml @17:01:09.36 Stroke completed -- waiting for pressure to equalize ... @17:01:09.37 SS.reconfigure :exhale @17:01:09.41 SS.hold @17:01:19.09 Intake.select :close @17:01:19.24 SSV.dial :exhaust @17:01:20.00 Exhaust.select :open @17:01:20.11 SS.seek 0ml @17:01:28.89 Exhaust.select :close @17:01:29.00 SSV.dial CBV @17:01:29.02 CBV.dial SSV @17:01:29.80 Allowing 130s for 25.0ml of remaining 900.0ml @17:01:29.81 Intake.select :open @17:01:31.97 Intake @15.3psia, vacuum from 9.8 to 12.8psi @17:01:31.99 SS.reconfigure :inhaling 1.30ml/s @17:01:32.03 SS.seek 25ml @17:01:32.13 recoverPRVreagent08 @17:01:32.63 PTV.dial :mixing @17:01:33.42 PBV.dial :waste1 @17:01:33.79 delay 30 seconds @17:01:45.33 Vacuum too high -- waiting up to 40 seconds @17:01:45.34 SS.reconfigure :exhale @17:01:45.39 SS.hold @17:01:49.63 Intake @13.6psia, vacuum from 8.1 to 11.1psi @17:01:49.65 SS.reconfigure :inhaling 1.17ml/s @17:01:49.69 SS.seek 25ml @17:01:59.59 Stroke completed -- waiting for pressure to equalize ... @17:01:59.60 SS.reconfigure :exhale @17:01:59.65 SS.hold @17:02:03.81 PBV.dial between PSV and waste1 @17:02:04.19 PSV.dial :air @17:02:04.66 PS.reconfigure :fast @17:02:04.71 PS.seek 2.5ml @17:02:09.32 Intake.select :close @17:02:09.46 SSV.dial :exhaust @17:02:10.24 Exhaust.select :open @17:02:10.36 SS.seek 0ml @17:02:19.11 Exhaust.select :close @17:02:19.22 SSV.dial CBV @17:02:19.26 CBV.dial SSV @17:02:20.02 Allowing 130s for 25.0ml of remaining 875.0ml @17:02:20.03 Intake.select :open @17:02:22.19 Intake @15.3psia, vacuum from 9.8 to 12.8psi @17:02:22.20 SS.reconfigure :inhaling 1.17ml/s @17:02:22.25 SS.seek 25ml @17:02:28.59 PSV.dial :mixloop @17:02:29.07 delay 2 seconds @17:02:31.09 PSV.dial :air @17:02:31.70 delay 2 seconds @17:02:33.72 PSV.dial :mixloop @17:02:34.17 delay 2 seconds @17:02:36.18 PSV.dial :air @17:02:36.78 PS.seek 0ml @17:02:37.32 Vacuum too high -- waiting up to 40 seconds @17:02:37.34 SS.reconfigure :exhale @17:02:37.38 SS.hold @17:02:42.26 Intake @13.8psia, vacuum from 8.3 to 11.3psi @17:02:42.28 SS.reconfigure :inhaling 1.06ml/s @17:02:42.33 SS.seek 25ml @17:02:52.83 Stroke completed -- waiting for pressure to equalize ... @17:02:52.84 SS.reconfigure :exhale @17:02:52.88 SS.hold @17:02:59.93 washCycleDA #1 of 2 @17:02:59.94 PSR.select :air @17:02:59.96 PSV.dial PSR @17:03:01.47 Pulling 0.5ml of PSR.wash @17:03:01.48 PSR.select :wash @17:03:01.59 PS.reconfigure :default @17:03:01.63 PS.seek 0.5ml @17:03:02.57 Intake.select :close @17:03:02.71 SSV.dial :exhaust @17:03:03.98 Exhaust.select :open @17:03:04.10 SS.seek 0ml @17:03:07.50 delay 5 seconds @17:03:12.52 PSR.select :air @17:03:12.63 PS.seek 2ml @17:03:12.84 Exhaust.select :close @17:03:12.95 SSV.dial CBV @17:03:12.97 CBV.dial SSV @17:03:13.77 Allowing 130s for 25.0ml of remaining 850.0ml @17:03:13.78 Intake.select :open @17:03:15.93 Intake @15.2psia, vacuum from 9.7 to 12.7psi @17:03:15.95 SS.reconfigure :inhaling 1.06ml/s @17:03:16.00 SS.seek 25ml @17:03:28.81 PTV.dial :puck @17:03:29.56 PSV.dial PTV @17:03:30.33 PBV.dial :waste1 @17:03:30.77 PS.seek 1.5ml @17:03:35.16 Vacuum too high -- waiting up to 40 seconds @17:03:35.18 SS.reconfigure :exhale @17:03:35.22 SS.hold @17:03:36.71 delay 3 seconds @17:03:39.73 PBV.dial between PSV and waste1 @17:03:40.11 PS.seek 0ml @17:03:40.89 Intake @13.9psia, vacuum from 8.4 to 11.4psi @17:03:40.91 SS.reconfigure :inhaling 0.95ml/s @17:03:40.95 SS.seek 25ml @17:03:49.50 Stroke completed -- waiting for pressure to equalize ... @17:03:49.51 SS.reconfigure :exhale @17:03:49.55 SS.hold @17:03:56.31 delay 2:00 @17:03:59.33 Intake.select :close @17:03:59.47 SSV.dial :exhaust @17:04:00.24 Exhaust.select :open @17:04:00.35 SS.seek 0ml @17:04:09.14 Exhaust.select :close @17:04:09.25 SSV.dial CBV @17:04:09.28 CBV.dial SSV @17:04:10.03 Allowing 130s for 25.0ml of remaining 825.0ml @17:04:10.04 Intake.select :open @17:04:12.20 Intake @15.1psia, vacuum from 9.6 to 12.6psi @17:04:12.22 SS.reconfigure :inhaling 0.95ml/s @17:04:12.26 SS.seek 25ml @17:04:31.73 Vacuum too high -- waiting up to 40 seconds @17:04:31.74 SS.reconfigure :exhale @17:04:31.78 SS.hold @17:04:38.30 Intake @14.2psia, vacuum from 8.7 to 11.7psi @17:04:38.32 SS.reconfigure :inhaling 0.87ml/s @17:04:38.36 SS.seek 25ml @17:04:48.86 Stroke completed -- waiting for pressure to equalize ... @17:04:48.87 SS.reconfigure :exhale @17:04:48.91 SS.hold @17:05:00.04 Intake.select :close @17:05:00.18 SSV.dial :exhaust @17:05:00.95 Exhaust.select :open @17:05:01.06 SS.seek 0ml @17:05:09.81 Exhaust.select :close @17:05:09.92 SSV.dial CBV @17:05:09.94 CBV.dial SSV @17:05:10.73 Allowing 130s for 25.0ml of remaining 800.0ml @17:05:10.75 Intake.select :open @17:05:12.90 Intake @15.2psia, vacuum from 9.7 to 12.7psi @17:05:12.92 SS.reconfigure :inhaling 0.87ml/s @17:05:12.96 SS.seek 25ml @17:05:35.19 Vacuum too high -- waiting up to 40 seconds @17:05:35.20 SS.reconfigure :exhale @17:05:35.25 SS.hold @17:05:41.75 Intake @14.2psia, vacuum from 8.7 to 11.7psi @17:05:41.77 SS.reconfigure :inhaling 0.78ml/s @17:05:41.81 SS.seek 25ml @17:05:53.09 Stroke completed -- waiting for pressure to equalize ... @17:05:53.10 SS.reconfigure :exhale @17:05:53.14 SS.hold @17:05:56.32 recoverPSRreagent08 @17:05:56.34 PTV.dial :puck @17:05:56.35 PBV.dial :waste1 @17:05:56.71 delay 30 seconds @17:06:04.44 Intake.select :close @17:06:04.58 SSV.dial :exhaust @17:06:05.32 Exhaust.select :open @17:06:05.44 SS.seek 0ml @17:06:14.43 Exhaust.select :close @17:06:14.54 SSV.dial CBV @17:06:14.56 CBV.dial SSV @17:06:15.34 Allowing 130s for 25.0ml of remaining 775.0ml @17:06:15.35 Intake.select :open @17:06:17.51 Intake @15.2psia, vacuum from 9.7 to 12.7psi @17:06:17.53 SS.reconfigure :inhaling 0.78ml/s @17:06:17.57 SS.seek 25ml @17:06:26.73 PBV.dial between PSV and waste1 @17:06:27.10 PSR.select :air @17:06:27.12 PSV.dial PSR @17:06:27.89 PS.reconfigure :fast @17:06:27.93 PS.seek 2.5ml @17:06:41.48 Vacuum too high -- waiting up to 40 seconds @17:06:41.49 SS.reconfigure :exhale @17:06:41.53 SS.hold @17:06:49.02 Intake @14.3psia, vacuum from 8.8 to 11.8psi @17:06:49.04 SS.reconfigure :inhaling 0.70ml/s @17:06:49.08 SS.seek 25ml @17:06:51.80 PSV.dial PTV @17:06:52.59 delay 5 seconds @17:06:57.61 PSV.dial PSR @17:06:58.37 delay 5 seconds @17:07:00.68 Stroke completed -- waiting for pressure to equalize ... @17:07:00.69 SS.reconfigure :exhale @17:07:00.74 SS.hold @17:07:03.43 PSV.dial PTV @17:07:04.22 delay 5 seconds @17:07:09.24 PSV.dial :air @17:07:10.01 PS.seek 0ml @17:07:13.66 Intake.select :close @17:07:13.80 SSV.dial :exhaust @17:07:14.56 Exhaust.select :open @17:07:14.67 SS.seek 0ml @17:07:23.42 Exhaust.select :close @17:07:23.53 SSV.dial CBV @17:07:23.56 CBV.dial SSV @17:07:24.31 Allowing 130s for 25.0ml of remaining 750.0ml @17:07:24.32 Intake.select :open @17:07:26.48 Intake @15.2psia, vacuum from 9.7 to 12.7psi @17:07:26.50 SS.reconfigure :inhaling 0.70ml/s @17:07:26.54 SS.seek 25ml @17:07:33.53 washCycleDA #2 of 2 @17:07:33.54 PSR.select :air @17:07:33.55 PSV.dial PSR @17:07:34.94 Pulling 0.5ml of PSR.wash @17:07:34.95 PSR.select :wash @17:07:35.06 PS.reconfigure :default @17:07:35.10 PS.seek 0.5ml @17:07:41.02 delay 5 seconds @17:07:46.04 PSR.select :air @17:07:46.15 PS.seek 2ml @17:07:51.60 Vacuum too high -- waiting up to 40 seconds @17:07:51.61 SS.reconfigure :exhale @17:07:51.65 SS.hold @17:07:59.13 Intake @14.3psia, vacuum from 8.8 to 11.8psi @17:07:59.15 SS.reconfigure :inhaling 0.63ml/s @17:07:59.19 SS.seek 25ml @17:08:02.24 PTV.dial :puck @17:08:02.26 PSV.dial PTV @17:08:03.01 PBV.dial :waste1 @17:08:03.42 PS.seek 1.5ml @17:08:09.33 delay 3 seconds @17:08:12.35 PBV.dial between PSV and waste1 @17:08:12.73 PS.seek 0ml @17:08:14.09 Stroke completed -- waiting for pressure to equalize ... @17:08:14.10 SS.reconfigure :exhale @17:08:14.14 SS.hold @17:08:28.41 Intake.select :close @17:08:28.56 SSV.dial :exhaust @17:08:28.93 delay 2:00 @17:08:29.31 Exhaust.select :open @17:08:29.42 SS.seek 0ml @17:08:38.43 Exhaust.select :close @17:08:38.54 SSV.dial CBV @17:08:38.56 CBV.dial SSV @17:08:39.34 Allowing 130s for 25.0ml of remaining 725.0ml @17:08:39.35 Intake.select :open @17:08:41.51 Intake @15.1psia, vacuum from 9.6 to 12.6psi @17:08:41.52 SS.reconfigure :inhaling 0.63ml/s @17:08:41.57 SS.seek 25ml @17:09:11.53 Vacuum too high -- waiting up to 40 seconds @17:09:12.03 SS.reconfigure :exhale @17:09:12.09 SS.hold @17:09:20.49 Intake @14.5psia, vacuum from 9.0 to 12.0psi @17:09:20.51 SS.reconfigure :inhaling 0.57ml/s @17:09:20.55 SS.seek 25ml @17:09:33.74 Stroke completed -- waiting for pressure to equalize ... @17:09:33.75 SS.reconfigure :exhale @17:09:33.80 SS.hold @17:09:50.01 Intake.select :close @17:09:50.15 SSV.dial :exhaust @17:09:50.93 Exhaust.select :open @17:09:51.04 SS.seek 0ml @17:09:59.82 Exhaust.select :close @17:09:59.93 SSV.dial CBV @17:09:59.95 CBV.dial SSV @17:10:00.74 Allowing 130s for 25.0ml of remaining 700.0ml @17:10:00.76 Intake.select :open @17:10:02.91 Intake @15.1psia, vacuum from 9.6 to 12.6psi @17:10:02.93 SS.reconfigure :inhaling 0.57ml/s @17:10:02.97 SS.seek 25ml @17:10:28.95 recoverPSRreagent08 @17:10:28.96 PTV.dial :puck @17:10:28.97 PBV.dial :waste1 @17:10:29.33 delay 30 seconds @17:10:33.97 Vacuum too high -- waiting up to 40 seconds @17:10:33.99 SS.reconfigure :exhale @17:10:34.03 SS.hold @17:10:43.79 Intake @14.5psia, vacuum from 9.0 to 12.0psi @17:10:43.81 SS.reconfigure :inhaling 0.51ml/s @17:10:43.85 SS.seek 25ml @17:10:59.35 PBV.dial between PSV and waste1 @17:10:59.72 PSR.select :air @17:10:59.74 PSV.dial PSR @17:11:00.51 PS.reconfigure :fast @17:11:00.55 PS.seek 2.5ml @17:11:00.85 Stroke completed -- waiting for pressure to equalize ... @17:11:00.86 SS.reconfigure :exhale @17:11:00.90 SS.hold @17:11:17.12 Intake.select :close @17:11:17.26 SSV.dial :exhaust @17:11:18.04 Exhaust.select :open @17:11:18.15 SS.seek 0ml @17:11:24.49 PSV.dial PTV @17:11:25.25 delay 5 seconds @17:11:26.91 Exhaust.select :close @17:11:27.02 SSV.dial CBV @17:11:27.04 CBV.dial SSV @17:11:27.82 Allowing 130s for 25.0ml of remaining 675.0ml @17:11:27.83 Intake.select :open @17:11:30.00 Intake @15.1psia, vacuum from 9.6 to 12.6psi @17:11:30.02 SS.reconfigure :inhaling 0.51ml/s @17:11:30.06 SS.seek 25ml @17:11:30.27 PSV.dial PSR @17:11:31.04 delay 5 seconds @17:11:36.05 PSV.dial PTV @17:11:36.82 delay 5 seconds @17:11:41.84 PSV.dial :air @17:11:42.62 PS.seek 0ml @17:12:03.34 Vacuum too high -- waiting up to 40 seconds @17:12:03.35 SS.reconfigure :exhale @17:12:03.40 SS.hold @17:12:05.95 getPMLReagent08 @17:12:05.96 PRV.dial :air @17:12:06.42 delay 5 seconds @17:12:11.44 PTV.dial :PRVmixing @17:12:12.78 delay 5 seconds @17:12:13.17 Intake @14.6psia, vacuum from 9.1 to 12.1psi @17:12:13.19 SS.reconfigure :inhaling 0.47ml/s @17:12:13.23 SS.seek 25ml @17:12:17.80 PRV.dial :ab2 @17:12:18.25 PTV.dial :PRVmixing @17:12:18.26 PSV.dial :mixloop @17:12:18.74 PS.reconfigure :default @17:12:18.78 PS.seek 0.3ml @17:12:22.73 delay 2 seconds @17:12:24.75 PRV.dial :air @17:12:25.29 PS.seek 0.55ml @17:12:28.64 addPMLReagent08 @17:12:28.65 PSV.dial :air @17:12:29.12 PS.seek 2ml @17:12:32.85 Stroke completed -- waiting for pressure to equalize ... @17:12:32.86 SS.reconfigure :exhale @17:12:32.90 SS.hold @17:12:44.76 PSV.dial :PML @17:12:45.24 PTV.dial :mixing @17:12:46.00 PBV.dial :waste1 @17:12:46.42 PS.seek 1.85ml @17:12:48.89 PBV.dial between PSV and waste1 @17:12:49.27 PS.seek 0ml @17:12:51.16 Intake.select :close @17:12:51.30 SSV.dial :exhaust @17:12:52.07 Exhaust.select :open @17:12:52.18 SS.seek 0ml @17:13:01.14 Exhaust.select :close @17:13:01.25 SSV.dial CBV @17:13:01.27 CBV.dial SSV @17:13:02.05 Allowing 130s for 25.0ml of remaining 650.0ml @17:13:02.06 Intake.select :open @17:13:04.22 Intake @15.2psia, vacuum from 9.7 to 12.7psi @17:13:04.24 SS.reconfigure :inhaling 0.47ml/s @17:13:04.28 SS.seek 25ml @17:13:08.98 delay 2 seconds @17:13:11.00 delay 8:00 @17:13:45.81 Vacuum too high -- waiting up to 40 seconds @17:13:46.24 SS.reconfigure :exhale @17:13:46.36 SS.hold @17:13:59.04 Intake @14.8psia, vacuum from 9.3 to 12.3psi @17:13:59.06 SS.reconfigure :inhaling 0.43ml/s @17:13:59.10 SS.seek 25ml @17:14:14.39 Stroke completed -- waiting for pressure to equalize ... @17:14:14.40 SS.reconfigure :exhale @17:14:14.45 SS.hold @17:14:32.69 Intake.select :close @17:14:32.84 SSV.dial :exhaust @17:14:33.60 Exhaust.select :open @17:14:33.71 SS.seek 0ml @17:14:42.52 Exhaust.select :close @17:14:42.62 SSV.dial CBV @17:14:42.64 CBV.dial SSV @17:14:43.41 Allowing 130s for 25.0ml of remaining 625.0ml @17:14:43.42 Intake.select :open @17:14:45.58 Intake @15.2psia, vacuum from 9.7 to 12.7psi @17:14:45.60 SS.reconfigure :inhaling 0.43ml/s @17:14:45.64 SS.seek 25ml @17:15:26.24 Vacuum too high -- waiting up to 40 seconds @17:15:26.25 SS.reconfigure :exhale @17:15:26.30 SS.hold @17:15:38.98 Intake @14.8psia, vacuum from 9.3 to 12.3psi @17:15:39.00 SS.reconfigure :inhaling 0.38ml/s @17:15:39.04 SS.seek 25ml @17:16:03.03 Stroke completed -- waiting for pressure to equalize ... @17:16:03.04 SS.reconfigure :exhale @17:16:03.10 SS.hold @17:16:25.54 Intake.select :close @17:16:25.68 SSV.dial :exhaust @17:16:26.44 Exhaust.select :open @17:16:26.55 SS.seek 0ml @17:16:35.53 Exhaust.select :close @17:16:35.64 SSV.dial CBV @17:16:35.66 CBV.dial SSV @17:16:36.44 Allowing 130s for 25.0ml of remaining 600.0ml @17:16:36.45 Intake.select :open @17:16:38.61 Intake @15.2psia, vacuum from 9.7 to 12.7psi @17:16:38.63 SS.reconfigure :inhaling 0.38ml/s @17:16:38.67 SS.seek 25ml @17:17:33.77 Vacuum too high -- waiting up to 40 seconds @17:17:33.78 SS.reconfigure :exhale @17:17:33.83 SS.hold @17:17:48.23 Intake @14.8psia, vacuum from 9.3 to 12.3psi @17:17:48.25 SS.reconfigure :inhaling 0.34ml/s @17:17:48.29 SS.seek 25ml @17:18:03.54 Stroke completed -- waiting for pressure to equalize ... @17:18:03.55 SS.reconfigure :exhale @17:18:03.60 SS.hold @17:18:25.93 Intake.select :close @17:18:26.07 SSV.dial :exhaust @17:18:26.84 Exhaust.select :open @17:18:26.95 SS.seek 0ml @17:18:35.92 Exhaust.select :close @17:18:36.02 SSV.dial CBV @17:18:36.04 CBV.dial SSV @17:18:36.81 Allowing 130s for 25.0ml of remaining 575.0ml @17:18:36.82 Intake.select :open @17:18:38.98 Intake @15.2psia, vacuum from 9.7 to 12.7psi @17:18:39.00 SS.reconfigure :inhaling 0.34ml/s @17:18:39.04 SS.seek 25ml @17:19:47.89 Vacuum too high -- waiting up to 40 seconds @17:19:47.90 SS.reconfigure :exhale @17:19:47.95 SS.hold @17:20:06.29 Intake @15.0psia, vacuum from 9.5 to 12.5psi @17:20:06.30 SS.reconfigure :inhaling 0.31ml/s @17:20:06.35 SS.seek 25ml @17:20:17.93 Stroke completed -- waiting for pressure to equalize ... @17:20:17.95 SS.reconfigure :exhale @17:20:17.99 SS.hold @17:20:38.90 Intake.select :close @17:20:39.05 SSV.dial :exhaust @17:20:39.83 Exhaust.select :open @17:20:39.94 SS.seek 0ml @17:20:49.02 Exhaust.select :close @17:20:49.14 SSV.dial CBV @17:20:49.15 CBV.dial SSV @17:20:49.93 Allowing 130s for 25.0ml of remaining 550.0ml @17:20:49.95 Intake.select :open @17:20:52.10 Intake @15.2psia, vacuum from 9.7 to 12.7psi @17:20:52.12 SS.reconfigure :inhaling 0.31ml/s @17:20:52.16 SS.seek 25ml @17:21:11.02 recoverPRVreagent08 @17:21:11.03 PTV.dial :mixing @17:21:11.05 PBV.dial :waste1 @17:21:11.41 delay 30 seconds @17:21:41.44 PBV.dial between PSV and waste1 @17:21:41.80 PSV.dial :air @17:21:42.29 PS.reconfigure :fast @17:21:42.33 PS.seek 2.5ml @17:22:06.25 PSV.dial :mixloop @17:22:06.74 delay 2 seconds @17:22:08.76 PSV.dial :air @17:22:09.22 delay 2 seconds @17:22:11.24 PSV.dial :mixloop @17:22:11.72 delay 2 seconds @17:22:13.74 PSV.dial :air @17:22:14.34 PS.seek 0ml @17:22:21.52 Stroke completed -- waiting for pressure to equalize ... @17:22:21.53 SS.reconfigure :exhale @17:22:21.58 SS.hold @17:22:37.85 washCycleDA #1 of 3 @17:22:37.86 PSR.select :air @17:22:37.87 PSV.dial PSR @17:22:39.38 Pulling 0.5ml of PSR.wash @17:22:39.39 PSR.select :wash @17:22:39.51 PS.reconfigure :default @17:22:39.55 PS.seek 0.5ml @17:22:45.63 delay 5 seconds @17:22:50.64 PSR.select :air @17:22:50.75 PS.seek 2ml @17:22:59.96 SS.reconfigure :default @17:23:00.00 SS.hold @17:23:00.11 Intake.select :close @17:23:00.26 ShallowSampler::Clogged in WCR -- Filtering too Slowly after 475.0ml @17:23:00.27 SSV.dial :exhaust @17:23:01.03 Exhaust.select :open @17:23:01.14 SS.reconfigure :exhale @17:23:01.19 SS.seek 0ml @17:23:06.91 PTV.dial :puck @17:23:07.66 PSV.dial PTV @17:23:08.41 PBV.dial :waste1 @17:23:08.84 PS.seek 1.5ml @17:23:10.22 Exhaust.select :close @17:23:10.34 Sampled 475.0ml @17:23:10.87 Drying puck before fixing for 20:00 @17:23:10.88 CBV.dial :waste2b @17:23:11.64 delay 1:00 @17:23:14.78 delay 3 seconds @17:23:17.80 PBV.dial between PSV and waste1 @17:23:18.17 PS.seek 0ml @17:23:34.32 delay 2:00 @17:24:11.67 CSV.dial :air @17:24:12.13 CS.reconfigure :fast @17:24:12.18 CS.seek 10ml @17:24:36.56 Pumping wcr puck from tube 3 up to 35psi... @17:24:36.58 CBV.dial SSV @17:24:37.91 CBV.dial between CSV and waste2b @17:24:38.89 CSV.dial between air and CTV @17:24:39.21 CS.seek 7.29ml @17:24:46.16 CTV.dial CSV,avoiding: :air @17:24:46.18 CSV.dial CTV @17:24:47.02 CS.reconfigure :pressurize @17:24:47.07 CS.seek 0ml @17:25:05.65 CBV.dial :waste2b @17:25:05.97 CS.reconfigure :default @17:25:06.02 CS.seek 0ml @17:25:07.28 delay 1:00 @17:25:34.34 recoverPSRreagent08 @17:25:34.35 PTV.dial :puck @17:25:34.36 PBV.dial :waste1 @17:25:34.74 delay 30 seconds @17:26:04.76 PBV.dial between PSV and waste1 @17:26:05.13 PSR.select :air @17:26:05.14 PSV.dial PSR @17:26:05.91 PS.reconfigure :fast @17:26:05.96 PS.seek 2.5ml @17:26:07.30 CTV.dial :air,avoiding: :intake @17:26:08.64 Fix #1 of 2 (with RNAlater) for 10:00 @17:26:08.66 CSV.dial CSR @17:26:09.16 Pulling 2ml of CSR.RNAlater @17:26:09.17 CSR.select :RNAlater @17:26:09.28 CS.seek 2ml @17:26:15.31 delay 5 seconds @17:26:20.33 CSR.select :air @17:26:20.44 CS.reconfigure :fast @17:26:20.49 CS.seek 10ml @17:26:29.78 PSV.dial PTV @17:26:30.67 delay 5 seconds @17:26:35.69 PSV.dial PSR @17:26:36.44 delay 5 seconds @17:26:40.09 Pumping wcr puck from tube 3 up to 35psi... @17:26:40.11 CBV.dial SSV @17:26:41.41 CBV.dial between CSV and waste2b @17:26:41.46 PSV.dial PTV @17:26:42.40 CSV.dial between air and CTV @17:26:42.43 delay 5 seconds @17:26:43.04 CS.seek 7.204ml @17:26:47.45 PSV.dial :air @17:26:48.24 PS.seek 0ml @17:26:50.50 CTV.dial CSV,avoiding: :air @17:26:50.53 CSV.dial CTV @17:26:51.91 CS.reconfigure :pressurize @17:26:51.96 CS.seek 0ml @17:27:10.11 delay 10:00 @17:27:11.53 washCycleDA #2 of 3 @17:27:11.54 PSR.select :air @17:27:11.56 PSV.dial PSR @17:27:12.94 Pulling 0.5ml of PSR.wash @17:27:12.95 PSR.select :wash @17:27:13.06 PS.reconfigure :default @17:27:13.11 PS.seek 0.5ml @17:27:19.03 delay 5 seconds @17:27:24.05 PSR.select :air @17:27:24.17 PS.seek 2ml @17:27:40.28 PTV.dial :puck @17:27:40.29 PSV.dial PTV @17:27:41.04 PBV.dial :waste1 @17:27:41.48 PS.seek 1.5ml @17:27:47.47 delay 3 seconds @17:27:50.49 PBV.dial between PSV and waste1 @17:27:50.86 PS.seek 0ml @17:28:06.97 delay 2:00 @17:30:07.00 recoverPSRreagent08 PTV.dial :puck @17:30:07.01 PBV.dial :waste1 @17:30:07.38 delay 30 seconds @17:30:37.40 PBV.dial between PSV and waste1 @17:30:37.78 PSR.select :air @17:30:38.26 PSV.dial PSR @17:30:39.03 PS.reconfigure :fast @17:30:39.08 PS.seek 2.5ml @17:31:02.78 PSV.dial PTV @17:31:03.54 delay 5 seconds @17:31:08.56 PSV.dial PSR @17:31:09.32 delay 5 seconds @17:31:14.34 PSV.dial PTV @17:31:15.23 delay 5 seconds @17:31:20.25 PSV.dial :air @17:31:21.03 PS.seek 0ml @17:31:44.37 washCycleDA #3 of 3 @17:31:44.38 PSR.select :air @17:31:44.40 PSV.dial PSR @17:31:45.78 Pulling 0.5ml of PSR.wash @17:31:45.79 PSR.select :wash @17:31:45.91 PS.reconfigure :default @17:31:45.95 PS.seek 0.5ml @17:31:51.90 delay 5 seconds @17:31:56.92 PSR.select :air @17:31:57.04 PS.seek 2ml @17:32:13.18 PTV.dial :puck @17:32:13.19 PSV.dial PTV @17:32:13.93 PBV.dial :waste1 @17:32:14.37 PS.seek 1.5ml @17:32:20.24 delay 3 seconds @17:32:23.26 PBV.dial between PSV and waste1 @17:32:23.64 PS.seek 0ml @17:32:39.74 delay 2:00 @17:34:39.77 recoverPSRreagent08 @17:34:39.78 PTV.dial :puck @17:34:39.79 PBV.dial :waste1 @17:34:40.16 delay 30 seconds @17:35:10.19 PBV.dial between PSV and waste1 @17:35:10.55 PSR.select :air @17:35:10.57 PSV.dial PSR @17:35:11.34 PS.reconfigure :fast @17:35:11.38 PS.seek 2.5ml @17:35:35.27 PSV.dial PTV @17:35:36.04 delay 5 seconds @17:35:41.06 PSV.dial PSR @17:35:41.82 delay 5 seconds @17:35:46.84 PSV.dial PTV @17:35:47.62 delay 5 seconds @17:35:52.64 PSV.dial :air @17:35:53.51 PS.seek 0ml @17:36:16.64 Gate.power :camera,:ON @17:36:16.66 Mixing DA Substrates @17:36:16.67 PSR.select :air @17:36:16.68 PSV.dial PSR @17:36:18.24 Pulling 0.25ml of PSR.sub1 @17:36:18.26 PSR.select :sub1 @17:36:18.48 PS.reconfigure :default @17:36:18.59 PS.seek 0.25ml @17:36:22.00 delay 5 seconds @17:36:27.07 Pulling 0.25ml of PSR.sub2 @17:36:27.08 PSR.select :sub2 @17:36:27.19 PS.seek 0.5ml @17:36:30.52 delay 5 seconds @17:36:35.54 PSR.select :air @17:36:35.65 PS.seek 2ml @17:36:51.86 PTV.dial :puck @17:36:51.88 PSV.dial PTV @17:36:52.65 PBV.dial :waste1 @17:36:53.09 PS.seek 1.5ml @17:36:59.02 delay 3 seconds @17:37:02.04 PBV.dial between PSV and waste1 @17:37:02.42 PS.seek 0ml @17:37:10.13 CBV.dial :waste2b @17:37:10.46 CS.reconfigure :default @17:37:10.51 CS.seek 0ml @17:37:11.96 delay 1:00 @17:37:18.58 delay 10 seconds @17:37:28.61 recoverPSRreagent08 @17:37:28.62 PTV.dial :puck @17:37:28.63 PBV.dial :waste1 @17:37:29.01 delay 30 seconds @17:37:59.03 PBV.dial between PSV and waste1 @17:37:59.40 PSR.select :air @17:37:59.41 PSV.dial PSR @17:38:00.20 PS.reconfigure :fast @17:38:00.24 PS.seek 2.5ml @17:38:11.98 CTV.dial :air,avoiding: :intake @17:38:13.34 Fix #2 of 2 (with RNAlater) for 10:00 @17:38:13.35 CSV.dial CSR @17:38:13.97 Pulling 2ml of CSR.RNAlater @17:38:13.98 CSR.select :RNAlater @17:38:14.09 CS.seek 2ml @17:38:20.08 delay 5 seconds @17:38:24.39 PSV.dial PTV @17:38:25.10 CSR.select :air @17:38:25.18 delay 5 seconds @17:38:25.21 CS.reconfigure :fast @17:38:25.26 CS.seek 10ml @17:38:30.19 PSV.dial PSR @17:38:31.08 delay 5 seconds @17:38:36.10 PSV.dial PTV @17:38:36.85 delay 5 seconds @17:38:41.87 PSV.dial :air @17:38:42.76 PS.seek 0ml @17:38:45.18 Pumping wcr puck from tube 3 up to 35psi... @17:38:45.19 CBV.dial SSV @17:38:46.51 CBV.dial between CSV and waste2b @17:38:47.47 CSV.dial between air and CTV @17:38:48.58 CS.seek 7.221ml @17:38:55.90 CTV.dial CSV,avoiding: :air @17:38:55.92 CSV.dial CTV @17:38:57.32 CS.reconfigure :pressurize @17:38:57.37 CS.seek 0ml @17:39:06.39 Additional air clear @17:39:06.40 PSR.select :air @17:39:06.42 PSV.dial PSR @17:39:07.82 Pulling 2ml of PSR.air @17:39:07.83 PSR.select :air @17:39:07.86 PS.reconfigure :default @17:39:07.90 PS.seek 2ml @17:39:16.33 CSV.dial :air @17:39:16.80 CS.reconfigure :fast @17:39:16.85 CS.seek 10ml @17:39:29.08 delay 5 seconds @17:39:34.10 PTV.dial :puck @17:39:34.12 PSV.dial PTV @17:39:35.00 PBV.dial :waste1 @17:39:35.44 PS.seek 1.5ml @17:39:41.39 delay 3 seconds @17:39:41.57 CSV.dial between air and CTV @17:39:41.92 CS.seek 4.403ml @17:39:44.41 PBV.dial between PSV and waste1 @17:39:44.79 delay 1 second @17:39:45.80 PSV.dial :waste1 @17:39:46.85 PS.seek 0ml @17:39:55.68 CTV.dial CSV,avoiding: :air @17:39:55.70 CSV.dial CTV @17:39:56.01 CS.reconfigure :pressurize @17:39:56.06 CS.seek 0ml @17:40:03.10 Processing Puck Heater at 28C before imaging @17:40:03.11 PH.coast @17:40:03.23 Imaging da puck in Processing Clamp while storing it to tube 2 @17:40:03.39 SC.reconfigure :tight @17:40:03.43 SC.seek 2 @17:40:07.02 SC.configure :limp @17:40:07.21 Elbow.reconfigure :tight @17:40:07.26 Elbow.seek 2 @17:40:07.83 CSV.dial :air @17:40:08.29 CS.reconfigure :fast @17:40:08.34 CS.seek 10ml @17:40:09.02 Forearm.reconfigure :tight @17:40:09.07 Forearm.seek 2 + 450 counts @17:40:10.08 SE.reconfigure :pick @17:40:10.13 SE.seek :beamBreak @17:40:12.44 SE.configure :default @17:40:12.59 Elbow.reconfigure :limp @17:40:12.63 Forearm.reconfigure :loose @17:40:12.67 Forearm.seek 2 @17:40:12.88 Elbow.hold @17:40:12.94 Elbow.reconfigure :tight @17:40:13.03 SE.seek 12.11 Pucks below Top Plate @17:40:13.47 Forearm.force -20 @17:40:13.51 Forearm.seek 2 + 293 counts @17:40:13.81 Forearm.reconfigure :tight @17:40:13.85 Forearm.seek 2 + 450 counts @17:40:14.12 SE.seek 13.11 Pucks below Top Plate @17:40:14.64 Forearm.reconfigure :loose @17:40:14.68 Forearm.seek :retract @17:40:15.60 Elbow.seek PC @17:40:18.23 Elbow.reconfigure :loose @17:40:18.32 Forearm.seek PC @17:40:19.30 Hand.close @17:40:19.96 PC.seek :open @17:40:24.71 Forearm.seek :retract @17:40:25.68 Elbow.reconfigure :tight @17:40:25.72 Elbow.seek 2 @17:40:28.44 Forearm.reconfigure :tight @17:40:28.49 Forearm.seek 2 + 450 counts @17:40:29.46 Hand.open @17:40:30.65 SE.seek 12.76 Pucks below Top Plate @17:40:30.90 SE.reconfigure :seal @17:40:30.94 SE.seek 11.8 Pucks below Top Plate @17:40:31.48 Lights, camera, ACTION! @17:40:31.51 PuckCamera: snap -bin=2,2 -auto /var/log/esp/da23jan2317h275ml.tif @17:40:32.91 CSV.dial between air and CTV @17:40:33.25 CS.seek 6.28ml @17:40:42.59 CTV.dial CSV,avoiding: :air @17:40:42.61 CSV.dial CTV @17:40:42.94 CS.reconfigure :pressurize @17:40:42.99 CS.seek 0ml @17:40:50.99 Waiting >20 seconds for da to relinquish FlushPuck @17:40:54.15 SX HX9/16 (USB:12): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 19.341 seconds /var/log/esp/da23jan2317h275ml.tif: TIFF Upload Complete @17:40:54.18 PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da23jan2317h275ml15s.tif @17:40:58.67 delay 10:00 @17:41:09.85 SX HX9/16 (USB:12): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15 seconds /var/log/esp/midres/da23jan2317h275ml15s.tif: TIFF Upload Complete @17:41:10.40 PuckCamera: snap -bin=2,2 20.0 /var/log/esp/midres/da23jan2317h275ml20s.tif @17:41:31.07 SX HX9/16 (USB:12): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 20 seconds /var/log/esp/midres/da23jan2317h275ml20s.tif: TIFF Upload Complete @17:41:31.08 Gate.power :camera,:OFF @17:41:31.11 DAfans.email "19.341 sec auto-exposure",Subject: "da23jan2317h275ml images!" @17:41:31.48 Elbow.seek 2 @17:41:31.66 Forearm.seek 2 + 450 counts @17:41:31.75 SE.reconfigure :gently @17:41:31.80 SE.seek 12.52 Pucks below Top Plate @17:41:32.43 Elbow.reconfigure :limp @17:41:32.50 Forearm.reconfigure :loose @17:41:32.54 Forearm.seek 2 @17:41:32.83 Elbow.hold @17:41:32.89 Elbow.reconfigure :tight @17:41:32.95 SE.reconfigure :default @17:41:33.01 SE.seek 12.7 Pucks below Top Plate @17:41:33.43 Forearm.force -20 @17:41:33.47 Forearm.seek 2 + 279 counts @17:41:33.77 Forearm.reconfigure :tight @17:41:33.82 Forearm.seek 2 + 450 counts @17:41:34.11 SE.seek :down @17:41:35.36 Forearm.reconfigure :loose @17:41:35.45 Forearm.seek :retract @17:41:35.94 SE.stop @17:41:36.58 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @17:41:36.94 Elbow.seek FlushPuck::Garage @17:41:39.05 Elbow.reconfigure :loose @17:41:39.30 Forearm.seek :garage @17:41:40.33 Hand.close @17:41:41.24 Forearm.seek :retract @17:41:42.24 Elbow.reconfigure :tight @17:41:42.33 Elbow.seek PC @17:41:43.73 Elbow.reconfigure :loose @17:41:43.93 Forearm.reconfigure :tight @17:41:44.02 Forearm.seek PC @17:41:45.64 PC.seek :closed @17:41:50.37 Processing Clamp onSpring/offSpring for 16/26 samples, on/off torque 1.56 @17:41:50.53 Hand.open @17:41:52.43 Forearm.reconfigure :loose @17:41:52.52 Forearm.seek :retract @17:41:53.54 PSR.select :air @17:41:53.61 PSV.dial PSR @17:41:54.26 Pulling 0.5ml of PSR.flush @17:41:54.30 PSR.select :flush @17:41:54.52 PS.seek 0.5ml @17:42:00.61 delay 5 seconds @17:42:05.63 PSR.select :air @17:42:05.74 PS.seek 2.5ml @17:42:06.07 upsync @17:42:27.05 delay 1 second @17:42:28.06 PRV.dial :waste2,avoiding: :ab2 @17:42:29.95 PTV.dial :PRVmixing @17:42:31.28 PSV.dial :mixloop @17:42:32.33 PS.seek :empty @17:42:58.70 PSR.select :air @17:42:58.72 PSV.dial PSR @17:43:00.80 Pulling 0.5ml of PSR.flush @17:43:00.81 PSR.select :flush @17:43:00.93 PS.seek 0.5ml @17:43:06.83 delay 5 seconds @17:43:11.85 PSR.select :air @17:43:11.96 PS.seek 2.5ml @17:43:33.14 delay 1 second @17:43:34.16 PBV.dial :waste1 @17:43:34.53 PTV.dial :mixing @17:43:35.29 PSV.dial :mixloop @17:43:36.36 PS.seek :empty @17:44:02.76 PSV.dial PSR @17:44:03.83 PSR.select :flush @17:44:03.95 PS.seek 1ml @17:44:14.95 PSR.select :air @17:44:15.06 PS.seek 2.5ml @17:44:31.45 PSV.dial PTV @17:44:32.22 PTV.dial :puck @17:44:32.97 PBV.dial :waste1 @17:44:32.99 PS.seek 0ml @17:44:59.32 PSV.dial PSR @17:45:00.09 PSR.select :flush @17:45:00.21 PS.seek 0.5ml @17:45:06.07 PSR.select :air @17:45:06.19 PS.seek 2.5ml @17:45:27.51 PSV.dial PTV @17:45:28.29 PBV.dial PSV @17:45:28.86 PS.seek 0ml @17:45:55.17 PSV.dial PBV @17:45:55.64 PS.seek 2.5ml @17:46:22.04 PSV.dial :waste1 @17:46:22.80 PS.seek 0ml @17:46:49.23 PSV.dial PSR @17:46:49.70 PSR.select :air @17:46:49.72 PS.seek 2.5ml @17:47:16.04 PSV.dial PTV @17:47:16.79 PBV.dial PSV @17:47:16.81 PS.seek 0ml @17:47:43.23 PSV.dial PBV @17:47:43.70 PS.seek 2.5ml @17:48:10.04 PSV.dial :waste1 @17:48:10.80 PS.seek 0ml @17:48:37.32 PSV.dial PSR @17:48:37.79 PSR.select :flush @17:48:37.90 PS.seek 1ml @17:48:48.88 PSR.select :air @17:48:48.99 PS.seek 2.5ml @17:49:05.19 PSV.dial PTV @17:49:05.95 PTV.dial :puck @17:49:05.96 PBV.dial :waste1 @17:49:06.51 PS.seek 0ml @17:49:32.82 PSV.dial PSR @17:49:33.58 PSR.select :flush @17:49:33.70 PS.seek 0.5ml @17:49:39.57 PSR.select :air @17:49:39.68 PS.seek 2.5ml @17:50:00.91 PSV.dial PTV @17:50:01.68 PBV.dial PSV @17:50:02.25 PS.seek 0ml @17:50:28.79 PSV.dial PBV @17:50:29.26 PS.seek 2.5ml @17:50:55.66 PSV.dial :waste1 @17:50:56.42 PS.seek 0ml @17:50:58.69 CBV.dial :waste2b @17:50:59.03 CS.reconfigure :default @17:50:59.07 CS.seek 0ml @17:51:00.67 delay 1:00 @17:51:22.72 PSV.dial PSR @17:51:23.20 PSR.select :air @17:51:23.22 PS.seek 2.5ml @17:51:49.57 PSV.dial PTV @17:51:50.77 PBV.dial PSV @17:51:50.79 PS.seek 0ml @17:52:00.69 CTV.dial :air,avoiding: :intake @17:52:02.08 Storing wcr puck in Collection Clamp to tube 2 @17:52:02.31 SC.reconfigure :tight @17:52:02.35 SC.seek 2 @17:52:02.54 SC.configure :limp @17:52:02.75 Elbow.reconfigure :tight @17:52:02.79 Elbow.seek 2 @17:52:05.40 Forearm.reconfigure :tight @17:52:05.45 Forearm.seek 2 + 450 counts @17:52:06.48 SE.reconfigure :pick @17:52:06.54 SE.seek :beamBreak @17:52:08.81 SE.configure :default @17:52:08.96 Elbow.reconfigure :limp @17:52:09.01 Forearm.reconfigure :loose @17:52:09.05 Forearm.seek 2 @17:52:09.31 Elbow.hold @17:52:09.37 Elbow.reconfigure :tight @17:52:09.47 SE.seek 13.1 Pucks below Top Plate @17:52:09.93 Forearm.force -20 @17:52:09.97 Forearm.seek 2 + 287 counts @17:52:10.26 Forearm.reconfigure :tight @17:52:10.30 Forearm.seek 2 + 450 counts @17:52:10.52 SE.seek 14.1 Pucks below Top Plate @17:52:11.04 Forearm.reconfigure :loose @17:52:11.08 Forearm.seek :retract @17:52:12.05 Elbow.seek CC @17:52:13.68 Elbow.reconfigure :loose @17:52:13.77 Forearm.seek CC @17:52:14.73 Hand.close @17:52:15.33 CC.seek :open @17:52:17.16 PSV.dial PBV @17:52:17.76 PS.seek 2.5ml @17:52:20.04 Forearm.seek :retract @17:52:20.98 Elbow.reconfigure :tight @17:52:21.03 Elbow.seek 2 @17:52:22.84 Elbow.reconfigure :limp @17:52:22.89 Forearm.seek 2 @17:52:23.89 Elbow.hold @17:52:23.95 Elbow.reconfigure :tight @17:52:24.00 Hand.open @17:52:24.69 Forearm.force -20 @17:52:24.73 Forearm.seek 2 + 315 counts @17:52:25.03 Forearm.reconfigure :tight @17:52:25.07 Forearm.seek 2 + 450 counts @17:52:25.33 SE.seek :down @17:52:26.49 Forearm.reconfigure :loose @17:52:26.53 Forearm.seek :retract @17:52:27.09 SE.stop @17:52:27.52
Starting finalPurge @17:52:44.06 PSV.dial :waste1 @17:52:44.84 PS.seek 0ml @17:52:47.13
Waiting >20 seconds for psFlush to relinquish FlushPuck @17:53:11.26 Loading FlushPuck into Collection Clamp from Processing Clamp @17:53:11.43 Elbow.seek PC @17:53:14.02 Elbow.reconfigure :loose @17:53:14.11 Forearm.seek PC @17:53:15.54 Hand.close @17:53:16.20 PC.seek :open @17:53:20.90 Forearm.seek :retract @17:53:21.86 Elbow.reconfigure :tight @17:53:21.90 Elbow.seek CC @17:53:25.60 Elbow.reconfigure :loose @17:53:25.69 Forearm.reconfigure :tight @17:53:25.73 Forearm.seek CC @17:53:26.97 CC.seek :closed @17:53:31.49 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.25 @17:53:31.56 Hand.open @17:53:32.26 Forearm.reconfigure :loose @17:53:32.30 Forearm.seek :retract @17:53:33.25 Flushing the Collection Valve::Manifold @17:53:33.37 CSV.dial CSR @17:53:33.85 Pulling 5ml of CSR.flush @17:53:33.86 CSR.select :flush @17:53:33.98 CS.seek 5ml @17:53:47.79 delay 5 seconds @17:53:52.80 CSR.select :air @17:53:52.95 CS.reconfigure :fast @17:53:52.99 CS.seek 7.5ml @17:53:59.78 CSV.dial :waste @17:54:01.07 CS.reconfigure :default @17:54:01.11 CS.seek 0ml @17:54:21.10 Flushing lower manifold's waste1 port @17:54:21.22 CSV.dial :flush @17:54:21.67 CS.reconfigure :fast @17:54:21.71 CS.seek 5ml @17:54:34.35 delay 5 seconds @17:54:39.36 CSV.dial :air @17:54:39.86 CS.seek 7ml @17:54:45.41 CSV.dial CTV @17:54:45.43
CTV.dial CSV @17:54:46.80 CBV.dial :waste1 @17:54:47.57 CS.seek 0ml @17:55:04.22 Flushing lower manifold's waste2 port @17:55:04.34 CSV.dial :flush @17:55:05.07 CS.seek 5ml @17:55:17.81 delay 5 seconds @17:55:22.83 CSV.dial :air @17:55:23.35 CS.seek 7ml @17:55:28.88 CSV.dial CTV @17:55:28.90
CTV.dial CSV @17:55:29.33 CBV.dial :waste2 @17:55:30.38 CS.seek 0ml @17:55:47.13 Flushing lower manifold's waste2b port @17:55:47.25 CSV.dial :flush @17:55:47.98 CS.seek 5ml @17:56:00.60 delay 5 seconds @17:56:05.61 CSV.dial :air @17:56:06.12 CS.seek 7.001ml @17:56:11.72 CSV.dial CTV @17:56:11.74
CTV.dial CSV @17:56:12.19 CBV.dial :waste2b @17:56:13.24 CS.seek 0ml @17:56:30.13 Disinfecting external Intake with kill @17:56:30.24 CSV.dial CSR @17:56:30.85 Pulling 8ml of CSR.kill @17:56:30.86 CSR.select :kill @17:56:30.97 CS.seek 8ml @17:56:50.69 delay 5 seconds @17:56:55.71 CSR.select :air @17:56:55.82 CS.seek 10ml @17:57:01.54 CSV.dial CBV @17:57:01.55
CBV.dial CSV @17:57:02.63 CTV.dial :intake @17:57:03.39 Intake.select :open @17:57:03.50 CS.seek 2ml @17:57:22.40 Intake.select :close @17:57:22.50 CTV.dial :air @17:57:23.27 CS.reconfigure :default @17:57:23.31 CS.seek 0ml @17:57:29.28 CSV.dial CSR @17:57:30.33 Pulling 5ml of CSR.flush @17:57:30.34 CSR.select :flush @17:57:30.45 CS.seek 5ml @17:57:44.26 delay 5 seconds @17:57:49.27 CSR.select :air @17:57:49.38 CS.seek 10ml @17:58:02.94 CSV.dial CBV @17:58:03.94 CS.seek 0ml @17:58:30.78 Garaging FlushPuck in Collection Clamp @17:58:30.85 Elbow.reconfigure :tight @17:58:30.89 Elbow.seek CC @17:58:31.22 Elbow.reconfigure :loose @17:58:31.31 Forearm.seek CC @17:58:32.26 Hand.close @17:58:32.82 CC.seek :open @17:58:37.42 Forearm.seek :retract @17:58:38.37 Elbow.reconfigure :tight @17:58:38.41 Elbow.seek :garage @17:58:41.38 Elbow.reconfigure :loose @17:58:41.47 Forearm.reconfigure :tight @17:58:41.51 Forearm.seek :garage @17:58:42.46 Hand.open @17:58:43.13 Forearm.reconfigure :loose @17:58:43.17 Forearm.seek :retract @17:58:44.13 Completed [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] begun 15:53:16 @17:58:44.18 Asleep after completing DA_WCR_12323 assays @17:58:44.20 GoodNews.email "{1 => 33,\n 2 => 15,\n 3 => 18,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@17:52:26, 20% flow, 14.1C, 32% humidity, 14.5psia, 13.734V, 0.369A, 0.363A avg, 5.07W\nSampled 475.0ml",Subject: "Asleep after completing DA_WCR_12323 assays" @17:58:49.46 upsync @15:48:16.49UTC26-Jan-23
ESP2 v3.41 I2C Gateway v3.20 for core bus @15:48:19.05 Configuration for short puck jake 1/11/21 by brent@mbari.org /home/esp/esp2/type/shallow/jake/configure.rb @15:48:23.00 Polling Can every 10:00 @15:48:23.13 Cleanly exited at 15:45:54UTC26-Jan-23 @15:48:23.17 Loading /home/esp/esp2/mission/DA_WCR_12623.rb mission @15:48:23.22 GoodNews.email "Starting DA_WCR_12623 w/pucks in tube 3\n{1 => 33,\n 2 => 15,\n 3 => 18,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@15:48:22, 16% flow, 17.0C, 33% humidity, 14.7psia, 13.764V, 0.296A, 0.284A avg, 4.08W",Subject: "DA_WCR_12623 begun" @15:48:23.26
Preparing to sleep until + 00:00:05 @15:48:23.29 Asleep til + 00:00:05 @15:48:23.30 Continuing at 15:48 @15:48:23.59 Gate.power :core,:OFF @15:48:23.60 Gate.power :raw,:OFF @15:48:23.61 Shutdown @15:48:28.19
Powering core bus @15:48:28.59 GoodNews.email "Then, in tube 3: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2/mission/DA_WCR_12623.rb:14\n{1 => 33,\n 2 => 15,\n 3 => 18,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@15:48:23, 16% flow, 17.1C, 33% humidity, 14.7psia, 13.759V, 0.296A, 0.284A avg, 4.08W",Subject: "Asleep until 15:48" @15:48:30.39 upsync @15:48:33.69
Gate.power :raw,:ON @15:48:34.33 CS.configure :default @15:48:34.43 PS.configure :default @15:48:34.52 SS.configure :default @15:48:34.62 CC.configure :default @15:48:34.71 PC.configure :default @15:48:34.77 upsync @15:48:34.85
Forearm.configure :loose @15:48:34.94 Elbow.configure :loose @15:48:35.05 SC.configure :loose @15:48:35.13 SE.configure :default @15:48:35.18 CH.coast @15:48:35.23 CH.configure :default @15:48:35.26 PH.coast @15:48:35.31 PH.configure :default @15:48:35.34 All dwarf controllers running firmware version 3.85 @15:48:35.45 Valve.reset @15:48:35.70 Intake.select :close @15:48:35.72 Exhaust.select :close @15:48:35.75 CTV.dial :air @15:48:35.84 PSV.dial :air @15:48:35.92 CSV.dial :waste1 @15:48:36.00
Forearm.reconfigure :loose @15:48:36.04 Forearm.home.seek :retract @15:48:36.36 SSV.dial :spit,avoiding: :exhaust @15:48:36.77 Hand.close @15:48:36.80
Elbow.jog 1500 @15:48:36.95 PS.reconfigure :default @15:48:36.99 PS.home.seek 0ml @15:48:37.18 CS.reconfigure :fast @15:48:37.21 SS.reconfigure :default @15:48:37.30 CS.home.seek 0ml @15:48:37.33 SS.home.seek 0ml @15:48:37.45
Elbow.reconfigure :loose @15:48:37.49 Elbow.home.seek :clear @15:48:41.98 SE.reconfigure :default @15:48:42.03 SE.home.seek :down @15:48:42.61 SC.reconfigure :tight @15:48:42.65 SC.home.seek :initial @15:48:45.28 PS.stop @15:49:10.95
SC.configure :limp @15:49:11.00 Forearm.seek :retract @15:49:11.06 Elbow.seek :hammer @15:49:12.10 Forearm.reconfigure :tight @15:49:12.14 Forearm.seek retracted @15:49:12.36 PC.reconfigure :default @15:49:12.38
CC.reconfigure :default @15:49:12.47 CC.home.seek :open @15:49:12.49 PC.home.seek :open @15:49:16.03
Phase [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] @15:49:16.04 Notice.email "At 15:49:15 began in tube 3: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2/mission/DA_WCR_12623.rb:14\n{1 => 33,\n 2 => 15,\n 3 => 18,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@15:49:16, 17% flow, 17.1C, 33% humidity, 14.7psia, 13.749V, 0.321A, 0.309A avg, 4.41W",Subject: "Started DA_WCR_12623 phase initialPurge; da [1000, 1000] #da and wsr are run simultanelously" @15:49:16.07
Starting initialPurge @15:49:16.08 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @15:49:16.15 Hand.open @15:49:16.89 Elbow.reconfigure :tight @15:49:16.94 Elbow.seek FlushPuck::Garage @15:49:18.82 Elbow.reconfigure :loose @15:49:18.92 Forearm.reconfigure :loose @15:49:18.96 Forearm.seek :garage @15:49:19.99 Hand.close @15:49:20.76 Forearm.seek :retract @15:49:21.74 Elbow.reconfigure :tight @15:49:21.79 Elbow.seek CC @15:49:22.01 upsync @15:49:24.87
Elbow.reconfigure :loose @15:49:24.97 Forearm.reconfigure :tight @15:49:25.01 Forearm.seek CC @15:49:26.26 CC.seek :closed @15:49:30.86 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.37 @15:49:30.93 Hand.open @15:49:31.56 Forearm.reconfigure :loose @15:49:31.61 Forearm.seek :retract @15:49:32.55 Flushing the Collection Valve::Manifold @15:49:32.67 CSV.dial CSR @15:49:33.99 Pulling 5ml of CSR.flush @15:49:34.00 CSR.select :flush @15:49:34.11 CS.reconfigure :default @15:49:34.15 CS.seek 5ml @15:49:47.74 delay 5 seconds @15:49:52.75 CSR.select :air @15:49:52.90 CS.reconfigure :fast @15:49:52.94 CS.seek 7.5ml @15:49:59.65 CSV.dial :waste @15:50:00.93 CS.reconfigure :default @15:50:00.97 CS.seek 0ml @15:50:21.17 Purging external Intake @15:50:21.29 CSV.dial :flush @15:50:21.72 CS.reconfigure :fast @15:50:21.76 CS.seek 10ml @15:50:46.14 delay 5 seconds @15:50:51.16 CSV.dial CBV @15:50:51.22
CBV.dial CSV @15:50:51.90 CTV.dial :intake @15:50:52.70 Intake.select :open @15:50:52.81 CS.seek 0ml @15:51:16.32 Intake.select :close @15:51:16.42 CTV.dial :air @15:51:17.22 Flushing upper manifold @15:51:17.33 CSV.dial :flush @15:51:18.07 CS.seek 5ml @15:51:30.70 delay 5 seconds @15:51:35.72 CSV.dial :air @15:51:36.21 CS.seek 7ml @15:51:41.70 CSV.dial CBV @15:51:41.72
CBV.dial CSV @15:51:42.75 CTV.dial :air @15:51:42.77 CS.seek 0ml @15:51:59.26 delay 7 seconds @15:52:06.29 CS.seek 3ml @15:52:14.26 CSV.dial :waste @15:52:14.71 CS.seek 0ml @15:52:22.52 CSV.dial :flush @15:52:22.96 CS.seek 3ml @15:52:31.07 delay 5 seconds @15:52:36.09 CSV.dial :air @15:52:36.59 CS.seek 5ml @15:52:42.07 CSV.dial :air @15:52:42.09 CS.seek 0ml @15:52:54.14 delay 7 seconds @15:53:01.16 CS.seek 3ml @15:53:09.17 CSV.dial :waste @15:53:09.89 CS.seek 0ml @15:53:17.48 Garaging FlushPuck in Collection Clamp @15:53:17.55 Elbow.reconfigure :tight @15:53:17.59 Elbow.seek CC @15:53:17.69 Elbow.reconfigure :loose @15:53:17.78 Forearm.seek CC @15:53:18.76 Hand.close @15:53:19.38 CC.seek :open @15:53:24.03 Forearm.seek :retract @15:53:24.97 Elbow.reconfigure :tight @15:53:25.01 Elbow.seek :garage @15:53:27.96 Elbow.reconfigure :loose @15:53:28.05 Forearm.reconfigure :tight @15:53:28.10 Forearm.seek :garage @15:53:29.14 Hand.open @15:53:30.18 Forearm.reconfigure :loose @15:53:30.22 Forearm.seek :retract @15:53:31.18 da sampling at most 1000ml, wcr at most 1000ml @15:53:31.21 Loading sh1 puck from tube 3 into Collection Clamp @15:53:31.22 DAfans.email "Started da in tube 3 at 15:53:31UTC26-Jan-23\n{1 => 33,\n 2 => 15,\n 3 => 18,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@15:49:16, 17% flow, 17.1C, 33% humidity, 14.7psia, 13.749V, 0.321A, 0.309A avg, 4.41W",Subject: "da sampling at most 1000ml, wcr at most 1000ml" @15:53:31.47
SC.reconfigure :tight @15:53:31.52 SC.seek 3 @15:53:35.15 SC.configure :limp @15:53:35.55 Elbow.reconfigure :tight @15:53:35.59 Elbow.seek 3 @15:53:37.09 upsync @15:53:37.53
Forearm.reconfigure :tight @15:53:37.57 Forearm.seek 3 + 450 counts @15:53:38.58 SE.reconfigure :pick @15:53:38.63 SE.seek :beamBreak @15:53:40.61 SE.configure :default @15:53:40.76 Elbow.seek 3 @15:53:40.92 Forearm.seek 3 + 300 counts @15:53:41.17 Forearm.reconfigure :loose @15:53:41.21 Hand.close @15:53:41.89 SE.seek :down @15:53:42.08
Forearm.seek :retract @15:53:43.00 Elbow.seek CC @15:53:43.54 SE.stop @15:53:44.63
Elbow.reconfigure :loose @15:53:44.73 Forearm.reconfigure :tight @15:53:44.77 Forearm.seek CC @15:53:45.97 CC.seek :closed @15:53:50.52 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.26 @15:53:50.53 Hand.open @15:53:51.19 Forearm.reconfigure :loose @15:53:51.23 Forearm.seek :retract @15:53:52.19 collectSample 1000ml w/bubble point of 24psi @15:53:52.20 Priming 7ml @15:53:52.21 Intake.select :close @15:53:52.22 Exhaust.select :close @15:53:52.23 SS.hold @15:53:52.43 Calibrating pressure and temperature sensors @15:53:52.46 CTV.dial :air @15:53:52.47 SSV.dial :spit,avoiding: :exhaust @15:53:52.49 SS.reconfigure :fast @15:53:52.53 SS.seek 5ml @15:53:55.36 SSV.dial CBV @15:53:55.37
CBV.dial SSV @15:53:56.71 delay 3.8 seconds @15:54:00.62 SSV.dial :spit @15:54:01.93 SS.seek 0ml @15:54:04.88 SSV.dial CBV @15:54:06.19 CTV.dial :intake @15:54:06.97 SSV.dial CBV @15:54:06.99
CBV.dial SSV @15:54:07.01 Allowing 40s for 7.0ml of remaining 7.0ml @15:54:07.02 Intake.select :open @15:54:09.17 Intake @14.8psia, vacuum from 9.3 to 12.3psi @15:54:09.19 SS.reconfigure :inhaling 2.20ml/s @15:54:09.23 SS.seek 7ml @15:54:15.41 Stroke completed -- waiting for pressure to equalize ... @15:54:15.42 SS.reconfigure :exhale @15:54:15.46 SS.hold @15:54:18.45 Intake.select :close @15:54:18.58 SSV.dial :exhaust @15:54:19.35 Exhaust.select :open @15:54:19.46 SS.seek 0ml @15:54:23.40 Exhaust.select :close @15:54:23.50 Sampling 1000.0ml @15:54:23.51 Intake.select :close @15:54:23.52 Exhaust.select :close @15:54:23.53 SS.reconfigure :default @15:54:23.57 SS.hold @15:54:23.75 CTV.dial :intake @15:54:23.77 SSV.dial CBV @15:54:23.80
CBV.dial SSV @15:54:24.76 Allowing 130s for 25.0ml of remaining 1000.0ml @15:54:24.77 Intake.select :open @15:54:26.92 Intake @15.1psia, vacuum from 9.6 to 12.6psi @15:54:26.93 SS.reconfigure :inhaling 2.20ml/s @15:54:26.98 SS.seek 25ml @15:54:37.64 Vacuum too high -- waiting up to 40 seconds @15:54:37.65 SS.reconfigure :exhale @15:54:37.69 SS.hold @15:54:41.99 Intake @13.3psia, vacuum from 7.8 to 10.8psi @15:54:42.00 SS.reconfigure :inhaling 1.98ml/s @15:54:42.05 SS.seek 25ml @15:54:47.57 Stroke completed -- waiting for pressure to equalize ... @15:54:47.58 SS.reconfigure :exhale @15:54:47.62 SS.hold @15:54:54.02 Intake.select :close @15:54:54.15 SSV.dial :exhaust @15:54:54.91 Exhaust.select :open @15:54:55.02 SS.seek 0ml @15:55:03.74 Exhaust.select :close @15:55:03.84 SSV.dial CBV @15:55:03.86
CBV.dial SSV @15:55:04.63 Allowing 130s for 25.0ml of remaining 975.0ml @15:55:04.64 Intake.select :open @15:55:06.79 Intake @15.5psia, vacuum from 10.0 to 13.0psi @15:55:06.81 SS.reconfigure :inhaling 1.98ml/s @15:55:06.85 SS.seek 25ml @15:55:18.42 Vacuum too high -- waiting up to 40 seconds @15:55:18.43 SS.reconfigure :exhale @15:55:18.47 SS.hold @15:55:22.86 Intake @13.7psia, vacuum from 8.2 to 11.2psi @15:55:22.88 SS.reconfigure :inhaling 1.79ml/s @15:55:22.92 SS.seek 25ml @15:55:28.80 Stroke completed -- waiting for pressure to equalize ... @15:55:28.81 SS.reconfigure :exhale @15:55:28.85 SS.hold @15:55:37.37 Intake.select :close @15:55:37.51 SSV.dial :exhaust @15:55:38.27 Exhaust.select :open @15:55:38.38 SS.seek 0ml @15:55:47.16 Exhaust.select :close @15:55:47.26 SSV.dial CBV @15:55:47.28
CBV.dial SSV @15:55:48.05 Allowing 130s for 25.0ml of remaining 950.0ml @15:55:48.06 Intake.select :open @15:55:50.22 Intake @15.6psia, vacuum from 10.0 to 13.0psi @15:55:50.23 SS.reconfigure :inhaling 1.79ml/s @15:55:50.28 SS.seek 25ml @15:56:02.07 Vacuum too high -- waiting up to 40 seconds @15:56:02.08 SS.reconfigure :exhale @15:56:02.12 SS.hold @15:56:07.16 Intake @14.0psia, vacuum from 8.5 to 11.5psi @15:56:07.17 SS.reconfigure :inhaling 1.61ml/s @15:56:07.22 SS.seek 25ml @15:56:14.42 Stroke completed -- waiting for pressure to equalize ... @15:56:14.43 SS.reconfigure :exhale @15:56:14.47 SS.hold @15:56:22.92 Intake.select :close @15:56:23.06 SSV.dial :exhaust @15:56:23.82 Exhaust.select :open @15:56:23.92 SS.seek 0ml @15:56:32.92 Exhaust.select :close @15:56:33.03 SSV.dial CBV @15:56:33.05
CBV.dial SSV @15:56:33.82 Allowing 130s for 25.0ml of remaining 925.0ml @15:56:33.83 Intake.select :open @15:56:35.98 Intake @15.6psia, vacuum from 10.0 to 13.0psi @15:56:35.99 SS.reconfigure :inhaling 1.61ml/s @15:56:36.04 SS.seek 25ml @15:56:47.98 Vacuum too high -- waiting up to 40 seconds @15:56:47.99 SS.reconfigure :exhale @15:56:48.03 SS.hold @15:56:52.57 Intake @13.9psia, vacuum from 8.4 to 11.4psi @15:56:52.59 SS.reconfigure :inhaling 1.45ml/s @15:56:52.63 SS.seek 25ml @15:57:00.33 Stroke completed -- waiting for pressure to equalize ... @15:57:00.34 SS.reconfigure :exhale @15:57:00.38 SS.hold @15:57:11.53 Intake.select :close @15:57:11.66 SSV.dial :exhaust @15:57:12.42 Exhaust.select :open @15:57:12.53 SS.seek 0ml @15:57:21.34 Exhaust.select :close @15:57:21.45 SSV.dial CBV @15:57:21.47
CBV.dial SSV @15:57:22.24 Allowing 130s for 25.0ml of remaining 900.0ml @15:57:22.25 Intake.select :open @15:57:24.40 Intake @15.4psia, vacuum from 9.9 to 12.9psi @15:57:24.42 SS.reconfigure :inhaling 1.45ml/s @15:57:24.46 SS.seek 25ml @15:57:37.70 Vacuum too high -- waiting up to 40 seconds SS.reconfigure :exhale @15:57:37.74 SS.hold @15:57:42.75 Intake @14.0psia, vacuum from 8.5 to 11.5psi @15:57:42.77 SS.reconfigure :inhaling 1.30ml/s @15:57:42.81 SS.seek 25ml @15:57:51.27 Stroke completed -- waiting for pressure to equalize ... @15:57:51.28 SS.reconfigure :exhale @15:57:51.32 SS.hold @15:58:02.34 Intake.select :close @15:58:02.47 SSV.dial :exhaust @15:58:03.24 Exhaust.select :open @15:58:03.34 SS.seek 0ml @15:58:12.16 Exhaust.select :close @15:58:12.26 SSV.dial CBV @15:58:12.28
CBV.dial SSV @15:58:13.05 Allowing 130s for 25.0ml of remaining 875.0ml @15:58:13.06 Intake.select :open @15:58:15.21 Intake @15.4psia, vacuum from 9.9 to 12.9psi @15:58:15.23 SS.reconfigure :inhaling 1.30ml/s @15:58:15.27 SS.seek 25ml @15:58:30.19 Vacuum too high -- waiting up to 40 seconds @15:58:30.20 SS.reconfigure :exhale @15:58:30.25 SS.hold @15:58:36.81 Intake @14.2psia, vacuum from 8.7 to 11.7psi @15:58:36.82 SS.reconfigure :inhaling 1.17ml/s @15:58:36.87 SS.seek 25ml @15:58:45.51 Stroke completed -- waiting for pressure to equalize ... @15:58:45.52 SS.reconfigure :exhale @15:58:45.56 SS.hold @15:58:58.10 Intake.select :close @15:58:58.25 SSV.dial :exhaust @15:58:59.00 Exhaust.select :open @15:58:59.11 SS.seek 0ml @15:59:08.36 Exhaust.select :close @15:59:08.47 SSV.dial CBV @15:59:08.48
CBV.dial SSV @15:59:09.25 Allowing 130s for 25.0ml of remaining 850.0ml @15:59:09.26 Intake.select :open @15:59:11.42 Intake @15.3psia, vacuum from 9.8 to 12.8psi @15:59:11.44 SS.reconfigure :inhaling 1.17ml/s @15:59:11.48 SS.seek 25ml @15:59:26.23 Vacuum too high -- waiting up to 40 seconds SS.reconfigure :exhale @15:59:26.27 SS.hold @15:59:31.95 Intake @14.1psia, vacuum from 8.6 to 11.6psi @15:59:31.96 SS.reconfigure :inhaling 1.06ml/s @15:59:32.00 SS.seek 25ml @15:59:43.04 Stroke completed -- waiting for pressure to equalize ... @15:59:43.05 SS.reconfigure :exhale @15:59:43.10 SS.hold @15:59:57.35 Intake.select :close @15:59:57.48 SSV.dial :exhaust @15:59:58.22 Exhaust.select :open @15:59:58.32 SS.seek 0ml @16:00:07.12 Exhaust.select :close @16:00:07.23 SSV.dial CBV @16:00:07.27
CBV.dial SSV @16:00:07.99 Allowing 130s for 25.0ml of remaining 825.0ml @16:00:08.00 Intake.select :open @16:00:10.15 Intake @15.2psia, vacuum from 9.7 to 12.7psi @16:00:10.16 SS.reconfigure :inhaling 1.06ml/s @16:00:10.20 SS.seek 25ml @16:00:26.69 Vacuum too high -- waiting up to 40 seconds @16:00:26.70 SS.reconfigure :exhale @16:00:26.74 SS.hold @16:00:34.23 Intake @14.5psia, vacuum from 9.0 to 12.0psi @16:00:34.24 SS.reconfigure :inhaling 0.95ml/s @16:00:34.29 SS.seek 25ml @16:00:45.59 Stroke completed -- waiting for pressure to equalize ... @16:00:45.60 SS.reconfigure :exhale @16:00:45.64 SS.hold @16:01:01.81 Intake.select :close @16:01:01.95 SSV.dial :exhaust @16:01:02.69 Exhaust.select :open @16:01:02.79 SS.seek 0ml @16:01:11.62 Exhaust.select :close @16:01:11.73 SSV.dial CBV @16:01:11.78
CBV.dial SSV @16:01:12.49 Allowing 130s for 25.0ml of remaining 800.0ml @16:01:12.50 Intake.select :open @16:01:14.65 Intake @15.3psia, vacuum from 9.8 to 12.8psi @16:01:14.67 SS.reconfigure :inhaling 0.95ml/s @16:01:14.71 SS.seek 25ml @16:01:32.47 Vacuum too high -- waiting up to 40 seconds @16:01:32.48 SS.reconfigure :exhale @16:01:32.53 SS.hold @16:01:41.07 Intake @14.7psia, vacuum from 9.2 to 12.2psi @16:01:41.08 SS.reconfigure :inhaling 0.87ml/s @16:01:41.13 SS.seek 25ml @16:01:53.95 Stroke completed -- waiting for pressure to equalize ... @16:01:53.96 SS.reconfigure :exhale @16:01:54.49 SS.hold @16:02:10.60 Intake.select :close @16:02:10.73 SSV.dial :exhaust @16:02:11.48 Exhaust.select :open @16:02:11.59 SS.seek 0ml @16:02:20.39 Exhaust.select :close @16:02:20.49 SSV.dial CBV @16:02:20.51
CBV.dial SSV @16:02:21.26 Allowing 130s for 25.0ml of remaining 775.0ml @16:02:21.27 Intake.select :open @16:02:23.43 Intake @15.3psia, vacuum from 9.8 to 12.8psi @16:02:23.45 SS.reconfigure :inhaling 0.87ml/s @16:02:23.49 SS.seek 25ml @16:02:41.63 Vacuum too high -- waiting up to 40 seconds @16:02:41.64 SS.reconfigure :exhale @16:02:41.68 SS.hold @16:02:50.71 Intake @14.8psia, vacuum from 9.3 to 12.3psi @16:02:50.72 SS.reconfigure :inhaling 0.78ml/s @16:02:50.76 SS.seek 25ml @16:03:06.09 Stroke completed -- waiting for pressure to equalize ... @16:03:06.10 SS.reconfigure :exhale @16:03:06.14 SS.hold @16:03:24.36 Intake.select :close @16:03:24.50 SSV.dial :exhaust @16:03:25.24 Exhaust.select :open @16:03:25.35 SS.seek 0ml @16:03:34.23 Exhaust.select :close @16:03:34.33 SSV.dial CBV @16:03:34.35
CBV.dial SSV @16:03:35.12 Allowing 130s for 25.0ml of remaining 750.0ml @16:03:35.13 Intake.select :open @16:03:37.29 Intake @15.1psia, vacuum from 9.6 to 12.6psi @16:03:37.30 SS.reconfigure :inhaling 0.78ml/s @16:03:37.34 SS.seek 25ml @16:03:57.22 Vacuum too high -- waiting up to 40 seconds @16:03:57.23 SS.reconfigure :exhale @16:03:57.27 SS.hold @16:04:07.01 Intake @14.8psia, vacuum from 9.3 to 12.3psi @16:04:07.03 SS.reconfigure :inhaling 0.70ml/s @16:04:07.07 SS.seek 25ml @16:04:23.59 Stroke completed -- waiting for pressure to equalize ... @16:04:23.60 SS.reconfigure :exhale @16:04:23.64 SS.hold @16:04:43.90 Intake.select :close @16:04:44.04 SSV.dial :exhaust @16:04:44.80 Exhaust.select :open @16:04:44.90 SS.seek 0ml @16:04:53.74 Exhaust.select :close @16:04:53.85 SSV.dial CBV @16:04:53.90
CBV.dial SSV @16:04:54.62 Allowing 130s for 25.0ml of remaining 725.0ml @16:04:54.63 Intake.select :open @16:04:56.78 Intake @15.3psia, vacuum from 9.8 to 12.8psi @16:04:56.80 SS.reconfigure :inhaling 0.70ml/s @16:04:56.84 SS.seek 25ml @16:05:18.50 Vacuum too high -- waiting up to 40 seconds @16:05:18.51 SS.reconfigure :exhale @16:05:18.55 SS.hold @16:05:29.66 Intake @15.0psia, vacuum from 9.5 to 12.5psi @16:05:29.68 SS.reconfigure :inhaling 0.63ml/s @16:05:29.72 SS.seek 25ml @16:05:48.33 Stroke completed -- waiting for pressure to equalize ... @16:05:48.34 SS.reconfigure :exhale @16:05:48.38 SS.hold @16:06:12.76 Intake.select :close @16:06:12.90 SSV.dial :exhaust @16:06:13.65 Exhaust.select :open @16:06:13.76 SS.seek 0ml @16:06:22.82 Exhaust.select :close @16:06:22.92 SSV.dial CBV @16:06:22.94
CBV.dial SSV @16:06:23.71 Allowing 130s for 25.0ml of remaining 700.0ml @16:06:23.72 Intake.select :open @16:06:25.87 Intake @15.2psia, vacuum from 9.7 to 12.7psi @16:06:25.89 SS.reconfigure :inhaling 0.63ml/s @16:06:25.93 SS.seek 25ml @16:06:51.69 Vacuum too high -- waiting up to 40 seconds @16:06:51.70 SS.reconfigure :exhale @16:06:51.74 SS.hold @16:07:04.38 Intake @15.1psia, vacuum from 9.6 to 12.6psi @16:07:04.40 SS.reconfigure :inhaling 0.57ml/s @16:07:04.44 SS.seek 25ml @16:07:22.24 Stroke completed -- waiting for pressure to equalize ... @16:07:22.25 SS.reconfigure :exhale @16:07:22.29 SS.hold @16:07:46.63 Intake.select :close @16:07:46.77 SSV.dial :exhaust @16:07:48.02 Exhaust.select :open @16:07:48.13 SS.seek 0ml @16:07:56.86 Exhaust.select :close @16:07:56.97 SSV.dial CBV @16:07:57.00
CBV.dial SSV @16:07:57.76 Allowing 130s for 25.0ml of remaining 675.0ml @16:07:57.77 Intake.select :open @16:07:59.92 Intake @15.2psia, vacuum from 9.7 to 12.7psi @16:07:59.94 SS.reconfigure :inhaling 0.57ml/s @16:07:59.98 SS.seek 25ml @16:08:29.34 Vacuum too high -- waiting up to 40 seconds @16:08:29.35 SS.reconfigure :exhale @16:08:29.39 SS.hold @16:08:43.64 Intake @15.1psia, vacuum from 9.6 to 12.6psi @16:08:43.66 SS.reconfigure :inhaling 0.51ml/s @16:08:43.70 SS.seek 25ml @16:09:03.10 Stroke completed -- waiting for pressure to equalize ... @16:09:03.11 SS.reconfigure :exhale @16:09:03.15 SS.hold @16:09:27.61 Intake.select :close @16:09:27.74 SSV.dial :exhaust @16:09:28.50 Exhaust.select :open @16:09:28.61 SS.seek 0ml @16:09:37.38 Exhaust.select :close @16:09:37.48 SSV.dial CBV @16:09:37.50
CBV.dial SSV @16:09:38.27 Allowing 130s for 25.0ml of remaining 650.0ml @16:09:38.28 Intake.select :open @16:09:40.44 Intake @15.1psia, vacuum from 9.6 to 12.6psi @16:09:40.46 SS.reconfigure :inhaling 0.51ml/s @16:09:40.50 SS.seek 25ml @16:10:13.87 Vacuum too high -- waiting up to 40 seconds @16:10:13.88 SS.reconfigure :exhale @16:10:13.92 SS.hold @16:10:28.27 Intake @14.8psia, vacuum from 9.3 to 12.3psi @16:10:28.28 SS.reconfigure :inhaling 0.47ml/s @16:10:28.32 SS.seek 25ml @16:10:48.75 Stroke completed -- waiting for pressure to equalize ... @16:10:48.76 SS.reconfigure :exhale @16:10:48.80 SS.hold @16:11:15.77 Intake.select :close @16:11:15.90 SSV.dial :exhaust @16:11:16.66 Exhaust.select :open @16:11:16.77 SS.seek 0ml @16:11:25.58 Exhaust.select :close @16:11:25.68 SSV.dial CBV @16:11:25.70
CBV.dial SSV @16:11:26.47 Allowing 130s for 25.0ml of remaining 625.0ml @16:11:26.48 Intake.select :open @16:11:28.64 Intake @15.1psia, vacuum from 9.6 to 12.6psi @16:11:28.65 SS.reconfigure :inhaling 0.47ml/s @16:11:28.70 SS.seek 25ml @16:12:03.80 Vacuum too high -- waiting up to 40 seconds @16:12:03.81 SS.reconfigure :exhale @16:12:03.86 SS.hold @16:12:18.08 Intake @14.6psia, vacuum from 9.1 to 12.1psi @16:12:18.10 SS.reconfigure :inhaling 0.43ml/s @16:12:18.14 SS.seek 25ml @16:12:42.22 Stroke completed -- waiting for pressure to equalize ... @16:12:42.23 SS.reconfigure :exhale @16:12:42.27 SS.hold @16:13:10.80 Intake.select :close @16:13:10.94 SSV.dial :exhaust @16:13:11.70 Exhaust.select :open @16:13:11.81 SS.seek 0ml @16:13:20.68 Exhaust.select :close @16:13:20.79 SSV.dial CBV @16:13:20.81
CBV.dial SSV @16:13:21.58 Allowing 130s for 25.0ml of remaining 600.0ml @16:13:21.59 Intake.select :open @16:13:23.74 Intake @15.1psia, vacuum from 9.6 to 12.6psi @16:13:23.76 SS.reconfigure :inhaling 0.43ml/s @16:13:23.80 SS.seek 25ml @16:14:02.96 Vacuum too high -- waiting up to 40 seconds @16:14:02.97 SS.reconfigure :exhale @16:14:03.01 SS.hold @16:14:19.18 Intake @14.9psia, vacuum from 9.4 to 12.4psi @16:14:19.20 SS.reconfigure :inhaling 0.38ml/s @16:14:19.24 SS.seek 25ml @16:14:46.99 Stroke completed -- waiting for pressure to equalize ... @16:14:47.00 SS.reconfigure :exhale @16:14:47.04 SS.hold @16:15:17.56 Intake.select :close @16:15:17.70 SSV.dial :exhaust @16:15:18.47 Exhaust.select :open @16:15:18.57 SS.seek 0ml @16:15:27.37 Exhaust.select :close @16:15:27.47 SSV.dial CBV @16:15:27.49
CBV.dial SSV @16:15:28.26 Allowing 130s for 25.0ml of remaining 575.0ml @16:15:28.27 Intake.select :open @16:15:30.43 Intake @15.1psia, vacuum from 9.6 to 12.6psi @16:15:30.45 SS.reconfigure :inhaling 0.38ml/s @16:15:30.49 SS.seek 25ml @16:16:21.86 Vacuum too high -- waiting up to 40 seconds @16:16:21.87 SS.reconfigure :exhale @16:16:21.91 SS.hold @16:16:40.16 Intake @15.3psia, vacuum from 9.8 to 12.8psi @16:16:40.18 SS.reconfigure :inhaling 0.34ml/s @16:16:40.22 SS.seek 25ml @16:17:05.95 Stroke completed -- waiting for pressure to equalize ... @16:17:05.96 SS.reconfigure :exhale @16:17:06.00 SS.hold @16:17:36.56 Intake.select :close @16:17:36.70 SSV.dial :exhaust @16:17:37.45 Exhaust.select :open @16:17:37.55 SS.seek 0ml @16:17:46.41 Exhaust.select :close @16:17:46.52 SSV.dial CBV @16:17:46.57
CBV.dial SSV @16:17:47.28 Allowing 130s for 25.0ml of remaining 550.0ml @16:17:47.29 Intake.select :open @16:17:49.44 Intake @15.1psia, vacuum from 9.6 to 12.6psi @16:17:49.46 SS.reconfigure :inhaling 0.34ml/s @16:17:49.50 SS.seek 25ml @16:19:09.83 Vacuum too high -- waiting up to 40 seconds SS.reconfigure :exhale @16:19:09.87 SS.hold @16:19:32.27 Intake @15.2psia, vacuum from 9.7 to 12.7psi @16:19:32.29 SS.reconfigure :inhaling 0.31ml/s @16:19:32.33 SS.seek 25ml @16:19:41.88 Stroke completed -- waiting for pressure to equalize ... @16:19:41.89 SS.reconfigure :exhale @16:19:41.93 SS.hold @16:19:57.30 SS.reconfigure :default @16:19:57.33 SS.hold @16:19:57.44 Intake.select :close @16:19:57.58 ShallowSampler::Clogged in MAIN -- Filtering too Slowly after 475.0ml @16:19:57.59 SSV.dial :exhaust @16:19:58.33 Exhaust.select :open @16:19:58.44 SS.reconfigure :exhale @16:19:58.48 SS.seek 0ml @16:20:07.29 Exhaust.select :close @16:20:07.39 Sampled 475.0ml @16:20:07.40 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @16:20:07.41 Intake.select :close @16:20:07.47 CSV.dial :waste @16:20:07.53 CS.seek 0ml @16:20:07.61 SSV.dial CBV,avoiding: :exhaust @16:20:07.63
CBV.dial SSV @16:20:07.70 CSV.dial :air @16:20:08.43 CS.seek 10ml @16:20:09.51
CTV.dial :air @16:20:10.30 delay 15 seconds @16:20:25.32 SSV.dial :spit,avoiding: :exhaust @16:20:26.65 CTV.dial CSV,avoiding: :intake @16:20:26.69
SS.seek 0ml @16:20:26.80 SSV.dial CBV,avoiding: :exhaust @16:20:32.92 CBV.dial between SSV and waste2 @16:20:33.30 CTV.dial CSV,avoiding: :air @16:20:33.32
CSV.dial CTV @16:20:33.77 CS.reconfigure :pressurize @16:20:33.86 CS.seek 0ml @16:20:52.39 BubblePoint: Intake @39.2psia, Outlet @15.0psia @16:20:52.40 CBV.dial SSV @16:20:52.72 SS.reconfigure :default @16:20:52.77 SS.seek 10ml @16:20:58.36 delay 15 seconds @16:21:13.48 Evacuated: Intake @16.8psia, Outlet @9.5psia @16:21:13.49 SSV.dial :spit,avoiding: :exhaust @16:21:14.81 SS.reconfigure :exhale @16:21:14.85 SS.seek 0ml @16:21:20.22 SS.reconfigure :fast @16:21:20.26 SS.seek 5ml @16:21:23.06 SSV.dial CBV,avoiding: :exhaust @16:21:23.09
CBV.dial SSV @16:21:24.38 CTV.dial :air,avoiding: :intake @16:21:25.74 CSV.dial CTV @16:21:25.77
SS.seek 10ml @16:21:26.76 CSV.dial :air @16:21:27.21 CS.reconfigure :fast @16:21:27.25 CS.seek 0ml @16:21:28.26
SSV.dial :spit,avoiding: :exhaust @16:21:29.59 SS.reconfigure :exhale @16:21:29.63 SS.seek 0ml @16:21:34.82 CS.stop @16:21:34.87
SS.reconfigure :fast @16:21:34.91 SS.seek 5ml @16:21:37.75 SSV.dial CBV,avoiding: :exhaust @16:21:37.76
CBV.dial SSV @16:21:40.09 SSV.dial :spit,avoiding: :exhaust @16:21:41.40 SS.reconfigure :default @16:21:41.44 SS.seek 0ml @16:21:44.72 SS.stop @16:21:44.76 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @16:21:44.77 Intake.select :close @16:21:44.84 CSV.dial :waste @16:21:44.95 SSV.dial CBV,avoiding: :exhaust @16:21:44.97
CBV.dial SSV @16:21:45.61 CS.seek 0ml @16:21:45.68 CSV.dial :air @16:21:46.29
CTV.dial :air @16:21:46.69 delay 15 seconds @16:21:46.80 CS.seek 10ml @16:22:01.78
SSV.dial :spit,avoiding: :exhaust @16:22:03.09 CTV.dial CSV,avoiding: :intake @16:22:03.12
SS.reconfigure :exhale @16:22:03.21 SS.seek 0ml @16:22:03.58 SSV.dial CBV,avoiding: :exhaust @16:22:11.04 CBV.dial between SSV and waste2 @16:22:11.43 CTV.dial CSV,avoiding: :air @16:22:11.44
CSV.dial CTV @16:22:11.90 CS.reconfigure :pressurize @16:22:11.94 CS.seek 0ml @16:22:33.17 BubblePoint: Intake @38.1psia, Outlet @13.9psia @16:22:33.18 CBV.dial SSV @16:22:33.50 SS.reconfigure :default @16:22:33.55 SS.seek 10ml @16:22:39.07 delay 15 seconds @16:22:54.18 Evacuated: Intake @18.0psia, Outlet @11.5psia @16:22:54.19 SSV.dial :spit,avoiding: :exhaust @16:22:55.52 SS.reconfigure :exhale @16:22:55.57 SS.seek 0ml @16:23:01.01 SS.reconfigure :fast @16:23:01.05 SS.seek 5ml @16:23:03.82 SSV.dial CBV,avoiding: :exhaust @16:23:03.84
CBV.dial SSV @16:23:05.16 CTV.dial :air,avoiding: :intake @16:23:06.50 CSV.dial CTV @16:23:06.54
SS.seek 10ml @16:23:07.53 CSV.dial :air @16:23:07.99 CS.reconfigure :fast @16:23:08.03 CS.seek 0ml @16:23:08.93
SSV.dial :spit,avoiding: :exhaust @16:23:10.24 SS.reconfigure :exhale @16:23:10.29 SS.seek 0ml @16:23:13.20 CS.stop @16:23:15.56
SS.reconfigure :fast @16:23:15.60 SS.seek 5ml @16:23:18.45 SSV.dial CBV,avoiding: :exhaust @16:23:18.47
CBV.dial SSV @16:23:20.79 SSV.dial :spit,avoiding: :exhaust @16:23:22.12 SS.reconfigure :default @16:23:22.16 SS.seek 0ml @16:23:25.55 SS.stop @16:23:25.58 Lysing: Adding 2.0ml AqMeOH @16:23:25.59 CBV.dial between CSV and waste1,avoiding: :DBV @16:23:27.08 CTV.dial CSV @16:23:28.39 CSV.dial CSR @16:23:29.17 Pulling 1ml of CSR.AqMeOH @16:23:29.18 CSR.select :AqMeOH @16:23:29.29 CS.reconfigure :default @16:23:29.33 CS.seek 1ml @16:23:32.92 delay 5 seconds @16:23:37.94 CSR.select :air @16:23:38.09 CS.seek 6ml @16:23:51.70 delay 2 seconds @16:23:53.72 CSV.dial :waste2 @16:23:54.16 CS.reconfigure :fast @16:23:54.20 CS.seek 0ml @16:24:08.60 CSV.dial CSR @16:24:09.10 Pulling 2ml of CSR.AqMeOH CSR.select :AqMeOH @16:24:09.21 CS.reconfigure :default @16:24:09.25 CS.seek 2ml @16:24:15.26 delay 5 seconds @16:24:20.28 CSR.select :air @16:24:20.43 CS.seek 5ml @16:24:29.10 delay 2 seconds @16:24:31.12 CSV.dial CTV @16:24:31.55 CS.seek 1ml @16:24:42.96 Before lysing Collection Puck Heater at 16.6C/38.5psia @16:24:42.98 Heating Puck.from 3, :sh1 in Collection Clamp to 70C for 10:00 @16:24:43.00 CH.seek 70C,8:00 @16:26:41.81 CH.hold 10:00,70C @16:26:41.98 Begun lysing Collection Puck Heater at 68C/46.8psia @16:36:41.99 Ended lysing Collection Puck Heater at 70C/45.7psia @16:36:42.01 CS.seek 0ml @16:36:45.35 CTV.dial between spare and air,avoiding: :intake @16:36:46.38 CSV.dial :air @16:36:46.82 CS.seek 5ml @16:37:00.65 CSV.dial CTV @16:37:01.09 delay 2 seconds @16:37:03.11 CSV.dial :air @16:37:03.56 delay 2 seconds @16:37:05.58 CSV.dial CTV @16:37:06.03 delay 2 seconds @16:37:08.05 CSV.dial :air @16:37:08.50 delay 2 seconds @16:37:10.52 CS.seek 0ml @16:37:24.21 Puck @74.7psia after final lysis pressurization @16:37:24.22 Recovering Lysate into Collection syringe @16:37:24.23 CSV.dial CBV @16:37:25.23 CBV.dial CSV @16:37:25.61 CS.seek 4.999ml @16:37:39.21 delay 5 seconds @16:37:44.24 CTV.dial :air @16:37:44.76 CS.seek 6.999ml @16:37:50.72 delay 5 seconds @16:37:55.74 CSV.dial :air2 @16:37:56.18 CS.seek 10ml @16:38:04.75 CSV.dial between air and CTV,avoiding: CBV @16:38:05.89 Lysing Complete @16:38:05.96 Storing sh1 puck in Collection Clamp to tube 2 @16:38:06.12 SC.reconfigure :tight @16:38:06.16 SC.seek 2 @16:38:09.69 SC.configure :limp @16:38:09.89 Elbow.reconfigure :tight @16:38:09.93 Elbow.seek 2 @16:38:11.70 Forearm.reconfigure :tight @16:38:11.74 Forearm.seek 2 + 450 counts @16:38:12.76 SE.reconfigure :pick @16:38:12.81 SE.seek :beamBreak @16:38:14.95 SE.configure :default @16:38:15.09 Elbow.reconfigure :limp @16:38:15.13 Forearm.reconfigure :loose @16:38:15.17 Forearm.seek 2 @16:38:15.38 Elbow.hold @16:38:15.44 Elbow.reconfigure :tight @16:38:15.53 SE.seek 14.12 Pucks below Top Plate @16:38:15.97 Forearm.force -20 @16:38:16.00 Forearm.seek 2 + 305 counts @16:38:16.28 Forearm.reconfigure :tight @16:38:16.33 Forearm.seek 2 + 450 counts @16:38:16.55 SE.seek 15.12 Pucks below Top Plate @16:38:17.07 Forearm.reconfigure :loose @16:38:17.11 Forearm.seek :retract @16:38:18.05 Elbow.seek CC @16:38:19.70 Elbow.reconfigure :loose @16:38:19.79 Forearm.seek CC @16:38:20.81 Hand.close @16:38:21.47 CC.seek :open @16:38:26.47 Forearm.seek :retract @16:38:27.47 Elbow.reconfigure :tight @16:38:27.51 Elbow.seek 2 @16:38:29.28 Elbow.reconfigure :limp @16:38:29.33 Forearm.seek 2 @16:38:30.28 Elbow.hold @16:38:30.34 Elbow.reconfigure :tight @16:38:30.38 Hand.open @16:38:31.07 Forearm.force -20 @16:38:31.11 Forearm.seek 2 + 314 counts @16:38:31.40 Forearm.reconfigure :tight @16:38:31.44 Forearm.seek 2 + 450 counts @16:38:31.69 SE.seek :down @16:38:32.81
Forearm.reconfigure :loose @16:38:32.86 Forearm.seek :retract @16:38:33.36 SE.stop @16:38:33.78
Tranferring DA @16:38:33.79 Beginning samplePassC @16:38:33.80 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @16:38:33.99 Elbow.seek FlushPuck::Garage @16:38:35.85 Elbow.reconfigure :loose @16:38:35.94 Forearm.seek :garage @16:38:36.93 Hand.close @16:38:37.70 Forearm.seek :retract @16:38:38.72 Elbow.reconfigure :tight @16:38:38.76 Elbow.seek CC @16:38:41.70 Elbow.reconfigure :loose @16:38:41.79 Forearm.reconfigure :tight @16:38:41.84 Forearm.seek CC @16:38:43.09 CC.seek :closed @16:38:47.62 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.26 @16:38:47.69 Hand.open @16:38:48.40 Forearm.reconfigure :loose @16:38:48.44 Forearm.seek :retract @16:38:49.42 PSV.dial PSR @16:38:50.79 Pulling 1.5ml of PSR.flush @16:38:50.80 PSR.select :flush @16:38:50.92 PS.seek 1.5ml @16:39:07.04 delay 5 seconds @16:39:12.06 PSV.dial :waste1 @16:39:12.53 PS.seek 0ml @16:39:28.69 PSV.dial PSR @16:39:29.16 PSR.select :air @16:39:29.27 PS.seek 2.5ml @16:39:55.70 PRV.dial CBV,avoiding: :ab2 @16:39:55.72
CBV.dial PRV @16:39:57.39 PSV.dial :mixloop @16:39:58.43 CTV.dial :air,avoiding: :intake @16:39:58.44 PTV.dial :PRVmixing @16:39:59.76 PS.seek 0ml @16:40:26.10 PSV.dial :air @16:40:26.56 PS.seek 2.5ml @16:40:52.84 PSV.dial :mixloop @16:40:53.31 PS.seek 0ml @16:41:19.69 PSV.dial :air @16:41:20.15 PS.seek 2.5ml @16:41:46.44 PSV.dial :mixloop @16:41:46.90 PS.seek 0ml @16:42:13.28 Garaging FlushPuck in Collection Clamp @16:42:13.35 Elbow.reconfigure :tight @16:42:13.39 Elbow.seek CC @16:42:13.69 Elbow.reconfigure :loose @16:42:13.77 Forearm.seek CC @16:42:14.79 Hand.close @16:42:15.34 CC.seek :open @16:42:20.42 Forearm.seek :retract @16:42:21.42 Elbow.reconfigure :tight @16:42:21.46 Elbow.seek :garage @16:42:24.41 Elbow.reconfigure :loose @16:42:24.49 Forearm.reconfigure :tight @16:42:24.54 Forearm.seek :garage @16:42:25.62 Hand.open @16:42:26.27 Forearm.reconfigure :loose @16:42:26.31 Forearm.seek :retract @16:42:27.30 PSV.dial :mixloop @16:42:27.32 PTV.dial :PRVmixing @16:42:27.33 PRV.dial CBV @16:42:27.35
CBV.dial PRV @16:42:27.37 CTV.dial :air,avoiding: :intake @16:42:27.39 Loading lyfil puck from tube 3 into Collection Clamp @16:42:27.61 SC.reconfigure :tight @16:42:27.65 SC.seek 3 @16:42:31.24 SC.configure :limp @16:42:31.43 Elbow.reconfigure :tight @16:42:31.47 Elbow.seek 3 @16:42:33.42 Forearm.reconfigure :tight @16:42:33.46 Forearm.seek 3 + 450 counts @16:42:34.47 SE.reconfigure :pick @16:42:34.52 SE.seek :beamBreak @16:42:36.52 SE.configure :default @16:42:36.66 Elbow.seek 3 @16:42:36.83 Forearm.seek 3 + 300 counts @16:42:37.10 Forearm.reconfigure :loose @16:42:37.14 Hand.close @16:42:37.73 SE.seek :down @16:42:37.92
Forearm.seek :retract @16:42:38.85 Elbow.seek CC @16:42:39.45 SE.stop @16:42:40.49
Elbow.reconfigure :loose @16:42:40.58 Forearm.reconfigure :tight @16:42:40.62 Forearm.seek CC @16:42:41.88 CC.seek :closed @16:42:46.38 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.14 @16:42:46.39 Hand.open @16:42:47.03 Forearm.reconfigure :loose @16:42:47.07 Forearm.seek :retract @16:42:48.04 CTV.dial CSV,avoiding: :intake @16:42:49.40 CSV.dial CTV @16:42:49.70 PS.seek 2.5ml @16:43:16.32 CS.seek 4.5ml @16:43:31.15 delay 8 seconds @16:43:39.17 PSV.dial :air @16:43:39.63 delay 2 seconds @16:43:41.64 PSV.dial :waste2 @16:43:42.38 PS.seek 0ml @16:44:08.65 CBV.dial between PRV and waste1 @16:44:08.98 CSV.dial :air @16:44:09.44 CS.seek 10ml @16:44:24.37 CSV.dial CTV @16:44:24.83 CS.seek 0ml @16:44:51.24 CBV.dial :waste1 @16:44:51.54 delay 5 seconds @16:44:56.56 CTV.dial :air,avoiding: :intake @16:44:57.96 Storing lyfil puck in Collection Clamp to tube 2 @16:44:58.12 SC.reconfigure :tight @16:44:58.16 SC.seek 2 @16:45:01.77 SC.configure :limp @16:45:02.41 Elbow.reconfigure :tight @16:45:02.47 Elbow.seek 2 @16:45:04.23 Forearm.reconfigure :tight @16:45:04.27 Forearm.seek 2 + 450 counts @16:45:05.33 SE.reconfigure :pick @16:45:05.38 SE.seek :beamBreak @16:45:07.49 SE.configure :default @16:45:07.64 Elbow.reconfigure :limp @16:45:07.68 Forearm.reconfigure :loose @16:45:07.72 Forearm.seek 2 @16:45:07.91 Elbow.hold @16:45:07.97 Elbow.reconfigure :tight @16:45:08.06 SE.seek 15.12 Pucks below Top Plate @16:45:08.51 Forearm.force -20 @16:45:08.55 Forearm.seek 2 + 300 counts @16:45:08.83 Forearm.reconfigure :tight @16:45:08.87 Forearm.seek 2 + 450 counts @16:45:09.08 SE.seek 16.12 Pucks below Top Plate @16:45:09.62 Forearm.reconfigure :loose @16:45:09.66 Forearm.seek :retract @16:45:10.56 Elbow.seek CC @16:45:12.23 Elbow.reconfigure :loose @16:45:12.32 Forearm.seek CC @16:45:13.29 Hand.close @16:45:13.84 CC.seek :open @16:45:18.56 Forearm.seek :retract @16:45:19.49 Elbow.reconfigure :tight @16:45:19.53 Elbow.seek 2 @16:45:21.32 Elbow.reconfigure :limp @16:45:21.36 Forearm.seek 2 @16:45:22.36 Elbow.hold @16:45:22.43 Elbow.reconfigure :tight @16:45:22.47 Hand.open @16:45:23.20 Forearm.force -20 @16:45:23.23 Forearm.seek 2 + 314 counts @16:45:23.62 Forearm.reconfigure :tight @16:45:23.67 Forearm.seek 2 + 450 counts @16:45:23.92 SE.seek :down @16:45:25.04
Forearm.reconfigure :loose @16:45:25.08 Forearm.seek :retract @16:45:25.51 SE.stop @16:45:26.07
PSV.dial :air @16:45:26.82 PS.seek 2.5ml @16:45:53.15 delay 1 second @16:45:54.17 PSV.dial :mixloop @16:45:54.61 delay 1 second @16:45:55.62 PSV.dial :air @16:45:56.08 delay 1 second @16:45:57.09 PSV.dial :mixloop @16:45:57.55 delay 1 second @16:45:58.56 PSV.dial :air @16:45:59.03 delay 1 second @16:46:00.05 PS.seek 0ml @16:46:26.41 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @16:46:26.57 Elbow.seek FlushPuck::Garage @16:46:28.37 Elbow.reconfigure :loose @16:46:28.46 Forearm.seek :garage @16:46:29.46 Hand.close @16:46:30.20 Forearm.seek :retract @16:46:31.18 Elbow.reconfigure :tight @16:46:31.22 Elbow.seek PC @16:46:32.46 Elbow.reconfigure :loose @16:46:32.56 Forearm.reconfigure :tight @16:46:32.60 Forearm.seek PC @16:46:33.82 PC.seek :closed @16:46:38.44 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.58 @16:46:38.50 Hand.open @16:46:39.14 Forearm.reconfigure :loose @16:46:39.19 Forearm.seek :retract @16:46:40.16 PRV.dial :air @16:46:40.61 PSV.dial :mixloop @16:46:41.14 PS.seek 0.3ml @16:46:45.09 PSV.dial PSR @16:46:46.18 Pulling 0.4ml of PSR.air PSR.select :air @16:46:46.21 PS.seek 0.7ml @16:46:51.06 delay 3 seconds @16:46:54.07 PSR.select :air @16:46:54.10 PS.seek 2.5ml @16:47:13.25 delay 3 seconds @16:47:16.26 PSV.dial :mixloop @16:47:17.32 PTV.dial :mixing @16:47:18.07 PBV.dial :waste2 @16:47:19.02 PS.seek 0ml @16:47:45.37 Garaging FlushPuck in Processing Clamp @16:47:45.49 Elbow.reconfigure :tight @16:47:45.53 Elbow.seek PC @16:47:45.63 Elbow.reconfigure :loose @16:47:45.72 Forearm.seek PC @16:47:46.70 Hand.close @16:47:47.34 PC.seek :open @16:47:52.04 Forearm.seek :retract @16:47:53.05 Elbow.reconfigure :tight @16:47:53.09 Elbow.seek :garage @16:47:54.45 Elbow.reconfigure :loose @16:47:54.54 Forearm.reconfigure :tight @16:47:54.58 Forearm.seek :garage @16:47:55.50 Hand.open @16:47:56.14 Forearm.reconfigure :loose @16:47:56.18 Forearm.seek :retract @16:47:57.15 Loading da puck from tube 3 into Processing Clamp @16:47:57.38 SC.reconfigure :tight @16:47:57.42 SC.seek 3 @16:48:01.00 SC.configure :limp @16:48:01.18 Elbow.reconfigure :tight @16:48:01.23 Elbow.seek 3 @16:48:03.10 Forearm.reconfigure :tight @16:48:03.14 Forearm.seek 3 + 450 counts @16:48:04.17 SE.reconfigure :pick @16:48:04.22 SE.seek :beamBreak @16:48:06.37 SE.configure :default @16:48:06.51 Elbow.seek 3 @16:48:06.68 Forearm.seek 3 + 300 counts @16:48:06.86 Forearm.reconfigure :loose @16:48:06.90 Hand.close @16:48:07.47 SE.seek :down @16:48:07.65
Forearm.seek :retract @16:48:08.58 Elbow.seek PC @16:48:09.21 SE.stop @16:48:11.10
Elbow.reconfigure :loose @16:48:11.20 Forearm.reconfigure :tight @16:48:11.24 Forearm.seek PC @16:48:12.42 PC.seek :closed @16:48:17.12 Processing Clamp onSpring/offSpring for 16/26 samples, on/off torque 1.61 @16:48:17.13 Hand.open @16:48:17.76 Forearm.reconfigure :loose @16:48:17.81 Forearm.seek :retract @16:48:18.83 Heating Puck.from 3, :da in Processing Clamp to 28C for 2:00:00 @16:48:18.85 PH.seek 28C,5:00 @16:48:56.05 PH.hold 2:00:00,28C @16:48:56.11 delay 1:30 @16:50:26.14 PSR.select :air @16:50:26.15 PSV.dial PSR @16:50:27.38 Pulling 0.5ml of PSR.wash PSR.select :wash @16:50:27.50 PS.seek 0.5ml @16:50:33.40 delay 5 seconds @16:50:38.42 PSR.select :air @16:50:38.53 PS.seek 2ml @16:50:54.71 PTV.dial :puck @16:50:55.45 PSV.dial PTV @16:50:56.30 PBV.dial :waste1 @16:50:57.71 PS.seek 1.5ml @16:51:03.62 delay 3 seconds @16:51:06.63 PBV.dial between PSV and waste1 @16:51:06.98 PS.seek 0ml @16:51:23.15 delay 5:00 @16:56:23.17 recoverPSRreagent08 @16:56:23.18 PTV.dial :puck @16:56:23.19 PBV.dial :waste1 @16:56:23.55 delay 30 seconds @16:56:53.57 PBV.dial between PSV and waste1 @16:56:53.92 PSR.select :air @16:56:53.94 PSV.dial PSR @16:56:54.68 PS.reconfigure :fast @16:56:54.72 PS.seek 2.5ml @16:57:18.42 PSV.dial PTV @16:57:19.17 delay 5 seconds @16:57:24.19 PSV.dial PSR @16:57:24.94 delay 5 seconds @16:57:29.96 PSV.dial PTV @16:57:30.70 delay 5 seconds @16:57:35.72 PSV.dial :air @16:57:36.60 PS.seek 0ml @16:57:59.67 PTV.dial :PRVmixing,via: PRV @16:58:00.98 PSV.dial :mixloop @16:58:01.44 PRV.dial :ab1 @16:58:02.18 PS.reconfigure :slow @16:58:02.22 PS.seek 0.15ml @16:58:05.73 delay 2 seconds @16:58:07.75 CBV.dial PRV @16:58:07.78
PRV.dial CBV @16:58:08.35 PS.seek 0.35ml @16:58:12.95 delay 2 seconds @16:58:14.97 CBV.dial between PRV and PSV @16:58:15.28 PRV.dial :air @16:58:15.71 PS.seek 0.9ml @16:58:27.42 PRV.dial :waste2 @16:58:28.16 PS.seek 0.5ml @16:58:36.86 addPMLReagent08 @16:58:36.87 PSV.dial :air @16:58:37.33 PS.reconfigure :default @16:58:37.37 PS.seek 2ml @16:58:53.58 PSV.dial :PML @16:58:54.03 PTV.dial :mixing @16:58:54.78 PBV.dial :waste1 @16:58:55.21 PS.seek 1.85ml @16:58:57.54 PBV.dial between PSV and waste1 @16:58:57.93 PS.seek 0ml @16:59:17.58 delay 2 seconds @16:59:19.59 Beginning samplePassC @16:59:19.61 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @16:59:19.77 Elbow.reconfigure :tight @16:59:19.81 Elbow.seek FlushPuck::Garage @16:59:21.24 Elbow.reconfigure :loose @16:59:21.32 Forearm.seek :garage @16:59:22.29 Hand.close @16:59:23.06 Forearm.seek :retract @16:59:24.03 Elbow.reconfigure :tight @16:59:24.07 Elbow.seek CC @16:59:27.00 Elbow.reconfigure :loose @16:59:27.09 Forearm.reconfigure :tight @16:59:27.13 Forearm.seek CC @16:59:28.88 CC.seek :closed @16:59:33.42 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.30 @16:59:33.49 Hand.open @16:59:34.13 Forearm.reconfigure :loose @16:59:34.17 Forearm.seek :retract @16:59:35.10 PSV.dial PSR @16:59:36.32 Pulling 1.5ml of PSR.flush @16:59:36.33 PSR.select :flush @16:59:36.45 PS.seek 1.5ml @16:59:52.61 delay 5 seconds @16:59:57.63 PSV.dial :waste1 @16:59:58.10 PS.seek 0ml @17:00:14.26 PSV.dial PSR @17:00:14.71 PSR.select :air @17:00:14.83 PS.seek 2.5ml @17:00:41.24 PRV.dial CBV,avoiding: :ab2 @17:00:41.25
CBV.dial PRV @17:00:42.89 PSV.dial :mixloop @17:00:43.92 CTV.dial :air,avoiding: :intake @17:00:43.93 PTV.dial :PRVmixing @17:00:44.69 PS.seek 0ml @17:01:11.10 PSV.dial :air @17:01:11.57 PS.seek 2.5ml @17:01:37.92 PSV.dial :mixloop @17:01:38.38 PS.seek 0ml @17:02:04.73 PSV.dial :air @17:02:05.19 PS.seek 2.5ml @17:02:31.54 PSV.dial :mixloop @17:02:32.00 PS.seek 0ml @17:02:58.54 Garaging FlushPuck in Collection Clamp @17:02:58.61 Elbow.reconfigure :tight @17:02:58.65 Elbow.seek CC @17:02:58.98 Elbow.reconfigure :loose @17:02:59.07 Forearm.seek CC @17:03:00.04 Hand.close @17:03:00.70 CC.seek :open @17:03:05.30 Forearm.seek :retract @17:03:06.26 Elbow.reconfigure :tight @17:03:06.30 Elbow.seek :garage @17:03:09.22 Elbow.reconfigure :loose @17:03:09.30 Forearm.reconfigure :tight @17:03:09.35 Forearm.seek :garage @17:03:10.37 Hand.open @17:03:11.00 Forearm.reconfigure :loose @17:03:11.05 Forearm.seek :retract @17:03:12.06 delayUntil "17:07:19.58" @17:03:12.08 Whole Cell Archive sampling 1000ml @17:03:12.10 Loading wcr puck from tube 3 into Collection Clamp @17:03:12.53 Elbow.reconfigure :tight @17:03:12.58 Elbow.seek 3 @17:03:14.50 Forearm.reconfigure :tight @17:03:14.54 Forearm.seek 3 + 450 counts @17:03:15.51 SE.reconfigure :pick @17:03:15.56 SE.seek :beamBreak @17:03:17.83 SE.configure :default @17:03:17.98 Elbow.seek 3 @17:03:18.16 Forearm.seek 3 + 300 counts @17:03:18.41 Forearm.reconfigure :loose @17:03:18.46 Hand.close @17:03:19.09 SE.seek :down @17:03:19.28 Forearm.seek :retract @17:03:20.24 Elbow.seek CC @17:03:20.92 SE.stop @17:03:22.28 Elbow.reconfigure :loose @17:03:22.38 Forearm.reconfigure :tight @17:03:22.42 Forearm.seek CC @17:03:23.67 CC.seek :closed @17:03:28.18 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.18 @17:03:28.20 Hand.open @17:03:28.90 Forearm.reconfigure :loose @17:03:28.94 Forearm.seek :retract @17:03:29.93 collectSample 1000ml w/bubble point of 35psi @17:03:29.94 Priming 7ml @17:03:29.95 Intake.select :close @17:03:29.96 Exhaust.select :close @17:03:29.98 SS.hold @17:03:30.17 Calibrating pressure sensors @17:03:30.18 CTV.dial :air @17:03:30.19 SSV.dial :spit,avoiding: :exhaust @17:03:30.21 SS.reconfigure :fast @17:03:30.25 SS.seek 5ml @17:03:32.95 SSV.dial CBV @17:03:32.96 CBV.dial SSV @17:03:34.30 delay 3.8 seconds @17:03:38.23 SSV.dial :spit @17:03:39.56 SS.seek 0ml @17:03:42.36 SSV.dial CBV @17:03:43.69 CTV.dial :intake @17:03:44.46 SSV.dial CBV @17:03:44.49 CBV.dial SSV @17:03:44.50 Allowing 40s for 7.0ml of remaining 7.0ml @17:03:44.51 Intake.select :open @17:03:46.67 Intake @14.9psia, vacuum from 9.4 to 12.4psi @17:03:46.69 SS.reconfigure :inhaling 2.20ml/s @17:03:46.73 SS.seek 7ml @17:03:53.83 Stroke completed -- waiting for pressure to equalize ... @17:03:53.84 SS.reconfigure :exhale @17:03:53.88 SS.hold @17:04:06.45 Intake.select :close @17:04:06.59 SSV.dial :exhaust @17:04:07.33 Exhaust.select :open @17:04:07.44 SS.seek 0ml @17:04:11.50 Exhaust.select :close @17:04:11.61 Sampling 1000.0ml @17:04:11.62 Intake.select :close @17:04:11.63 Exhaust.select :close @17:04:11.64 SS.reconfigure :default @17:04:11.68 SS.hold @17:04:11.88 CTV.dial :intake @17:04:11.90 SSV.dial CBV @17:04:11.93 CBV.dial SSV @17:04:12.68 Allowing 130s for 25.0ml of remaining 1000.0ml @17:04:12.69 Intake.select :open @17:04:14.84 Intake @15.6psia, vacuum from 10.0 to 13.0psi @17:04:14.86 SS.reconfigure :inhaling 2.20ml/s @17:04:14.90 SS.seek 25ml @17:04:22.03 Vacuum too high -- waiting up to 40 seconds @17:04:22.04 SS.reconfigure :exhale @17:04:22.08 SS.hold @17:04:27.05 Intake @14.8psia, vacuum from 9.3 to 12.3psi @17:04:27.07 SS.reconfigure :inhaling 1.98ml/s @17:04:27.11 SS.seek 25ml @17:04:33.77 Vacuum too high -- waiting up to 40 seconds @17:04:33.79 SS.reconfigure :exhale @17:04:33.83 SS.hold @17:04:40.59 Intake @15.1psia, vacuum from 9.6 to 12.6psi @17:04:40.61 SS.reconfigure :inhaling 1.79ml/s @17:04:40.65 SS.seek 25ml @17:04:44.29 Stroke completed -- waiting for pressure to equalize ... @17:04:44.30 SS.reconfigure :exhale @17:04:44.34 SS.hold @17:04:49.88 Intake.select :close @17:04:50.02 SSV.dial :exhaust @17:04:50.78 Exhaust.select :open @17:04:50.89 SS.seek 0ml @17:04:59.69 Exhaust.select :close @17:04:59.79 SSV.dial CBV @17:04:59.83 CBV.dial SSV @17:05:00.58 Allowing 130s for 25.0ml of remaining 975.0ml @17:05:00.59 Intake.select :open @17:05:02.75 Intake @16.2psia, vacuum from 10.0 to 13.0psi @17:05:02.77 SS.reconfigure :inhaling 1.79ml/s @17:05:02.81 SS.seek 25ml @17:05:10.30 Vacuum too high -- waiting up to 40 seconds @17:05:10.31 SS.reconfigure :exhale @17:05:10.36 SS.hold @17:05:13.53 Intake @14.9psia, vacuum from 9.4 to 12.4psi @17:05:13.55 SS.reconfigure :inhaling 1.61ml/s @17:05:13.59 SS.seek 25ml @17:05:24.30 Vacuum too high -- waiting up to 40 seconds @17:05:24.31 SS.reconfigure :exhale @17:05:24.36 SS.hold @17:05:33.06 Intake @15.4psia, vacuum from 9.9 to 12.9psi @17:05:33.08 SS.reconfigure :inhaling 1.45ml/s @17:05:33.12 SS.seek 25ml @17:05:34.70 Stroke completed -- waiting for pressure to equalize ... @17:05:34.71 SS.reconfigure :exhale @17:05:34.76 SS.hold @17:05:37.17 Intake.select :close @17:05:37.32 SSV.dial :exhaust @17:05:38.08 Exhaust.select :open @17:05:38.19 SS.seek 0ml @17:05:47.26 Exhaust.select :close @17:05:47.37 SSV.dial CBV @17:05:47.39 CBV.dial SSV @17:05:48.17 Allowing 130s for 25.0ml of remaining 950.0ml @17:05:48.19 Intake.select :open @17:05:50.34 Intake @16.0psia, vacuum from 10.0 to 13.0psi @17:05:50.84 SS.reconfigure :inhaling 1.45ml/s @17:05:50.89 SS.seek 25ml @17:06:01.77 Vacuum too high -- waiting up to 40 seconds @17:06:01.78 SS.reconfigure :exhale @17:06:01.83 SS.hold @17:06:05.50 Intake @15.1psia, vacuum from 9.6 to 12.6psi @17:06:05.52 SS.reconfigure :inhaling 1.30ml/s @17:06:05.56 SS.seek 25ml @17:06:16.14 Stroke completed -- waiting for pressure to equalize ... @17:06:16.15 SS.reconfigure :exhale @17:06:16.20 SS.hold @17:06:25.88 Intake.select :close @17:06:26.02 SSV.dial :exhaust @17:06:26.79 Exhaust.select :open @17:06:26.90 SS.seek 0ml @17:06:35.68 Exhaust.select :close @17:06:35.79 SSV.dial CBV @17:06:35.82 CBV.dial SSV @17:06:36.56 Allowing 130s for 25.0ml of remaining 925.0ml @17:06:36.57 Intake.select :open @17:06:38.73 Intake @16.1psia, vacuum from 10.0 to 13.0psi @17:06:38.75 SS.reconfigure :inhaling 1.30ml/s @17:06:38.79 SS.seek 25ml @17:06:50.52 Vacuum too high -- waiting up to 40 seconds @17:06:50.53 SS.reconfigure :exhale @17:06:50.58 SS.hold @17:06:54.47 Intake @14.9psia, vacuum from 9.4 to 12.4psi @17:06:54.49 SS.reconfigure :inhaling 1.17ml/s @17:06:54.53 SS.seek 25ml @17:07:06.59 Stroke completed -- waiting for pressure to equalize ... @17:07:06.60 SS.reconfigure :exhale @17:07:06.64 SS.hold @17:07:16.31 Intake.select :close @17:07:16.45 SSV.dial :exhaust @17:07:17.21 Exhaust.select :open @17:07:17.32 SS.seek 0ml @17:07:19.59 recoverPRVreagent08 @17:07:19.60 PTV.dial :mixing @17:07:20.35 PBV.dial :waste1 @17:07:20.72 delay 30 seconds @17:07:26.12 Exhaust.select :close @17:07:26.23 SSV.dial CBV @17:07:26.25 CBV.dial SSV @17:07:27.01 Allowing 130s for 25.0ml of remaining 900.0ml @17:07:27.03 Intake.select :open @17:07:29.18 Intake @15.9psia, vacuum from 10.0 to 13.0psi @17:07:29.20 SS.reconfigure :inhaling 1.17ml/s @17:07:29.24 SS.seek 25ml @17:07:43.08 Vacuum too high -- waiting up to 40 seconds @17:07:43.09 SS.reconfigure :exhale @17:07:43.14 SS.hold @17:07:48.20 Intake @15.2psia, vacuum from 9.7 to 12.7psi @17:07:48.22 SS.reconfigure :inhaling 1.06ml/s @17:07:48.26 SS.seek 25ml @17:07:50.74 PBV.dial between PSV and waste1 @17:07:51.12 PSV.dial :air @17:07:51.59 PS.reconfigure :fast @17:07:51.63 PS.seek 2.5ml @17:08:00.03 Stroke completed -- waiting for pressure to equalize ... @17:08:00.04 SS.reconfigure :exhale @17:08:00.08 SS.hold @17:08:11.12 Intake.select :close @17:08:11.26 SSV.dial :exhaust @17:08:12.03 Exhaust.select :open @17:08:12.14 SS.seek 0ml @17:08:15.53 PSV.dial :mixloop @17:08:16.01 delay 2 seconds @17:08:18.03 PSV.dial :air @17:08:18.49 delay 2 seconds @17:08:20.50 PSV.dial :mixloop @17:08:20.95 Exhaust.select :close @17:08:21.03 delay 2 seconds @17:08:21.06 SSV.dial CBV @17:08:21.12 CBV.dial SSV @17:08:21.84 Allowing 130s for 25.0ml of remaining 875.0ml @17:08:21.85 Intake.select :open @17:08:23.04 PSV.dial :air @17:08:23.51 PS.seek 0ml @17:08:24.01 Intake @15.7psia, vacuum from 10.0 to 13.0psi @17:08:24.03 SS.reconfigure :inhaling 1.06ml/s @17:08:24.08 SS.seek 25ml @17:08:41.27 Vacuum too high -- waiting up to 40 seconds @17:08:41.28 SS.reconfigure :exhale @17:08:41.32 SS.hold @17:08:48.02 I2C::Bus::Error in core_reader -- Unexpected message ACK byte: 0x01 @17:08:48.82 Polling Can every 10:00 @17:08:53.98 I2C::Request::Timeout in WCR -- No Response to I2C::Servo::Status3Request[54:->27] @17:08:53.99 Retry #1 of 3 @17:08:54.05 Intake @16.0psia, vacuum from 10.0 to 13.0psi @17:08:54.07 SS.reconfigure :inhaling 0.95ml/s @17:08:54.12 SS.seek 25ml @17:09:05.08 Stroke completed -- waiting for pressure to equalize ... @17:09:05.09 SS.reconfigure :exhale @17:09:05.13 SS.hold @17:09:14.80 Intake.select :close @17:09:14.94 SSV.dial :exhaust @17:09:15.71 Exhaust.select :open @17:09:15.82 SS.seek 0ml @17:09:24.62 Exhaust.select :close @17:09:24.72 SSV.dial CBV @17:09:24.74 CBV.dial SSV @17:09:25.51 Allowing 130s for 25.0ml of remaining 850.0ml @17:09:25.53 Intake.select :open @17:09:27.68 Intake @15.6psia, vacuum from 10.0 to 13.0psi @17:09:28.18 SS.reconfigure :inhaling 0.95ml/s @17:09:28.24 SS.seek 25ml @17:09:49.08 Vacuum too high -- waiting up to 40 seconds @17:09:49.09 SS.reconfigure :exhale @17:09:49.14 SS.hold @17:09:57.70 Intake @15.3psia, vacuum from 9.8 to 12.8psi @17:09:57.72 SS.reconfigure :inhaling 0.87ml/s @17:09:57.76 SS.seek 25ml @17:10:06.91 Stroke completed -- waiting for pressure to equalize ... @17:10:06.93 SS.reconfigure :exhale @17:10:06.97 SS.hold @17:10:18.01 Intake.select :close @17:10:18.15 SSV.dial :exhaust @17:10:18.92 Exhaust.select :open @17:10:19.03 SS.seek 0ml @17:10:28.07 Exhaust.select :close @17:10:28.18 SSV.dial CBV @17:10:28.20 CBV.dial SSV @17:10:28.98 Allowing 130s for 25.0ml of remaining 825.0ml @17:10:28.99 Intake.select :open @17:10:31.15 Intake @15.7psia, vacuum from 10.0 to 13.0psi @17:10:31.17 SS.reconfigure :inhaling 0.87ml/s @17:10:31.21 SS.seek 25ml @17:10:50.74 Vacuum too high -- waiting up to 40 seconds @17:10:50.75 SS.reconfigure :exhale @17:10:50.79 SS.hold @17:10:58.27 Intake @15.1psia, vacuum from 9.6 to 12.6psi @17:10:58.29 SS.reconfigure :inhaling 0.78ml/s @17:10:58.33 SS.seek 25ml @17:11:03.56 I2C::Request::Timeout in da -- No Response to I2C::Servo::AbsMove3Request[29:->25] @17:11:03.57 Retry #1 of 3 @17:11:03.63 washCycleDA #1 of 2 @17:11:03.64 PSR.select :air @17:11:03.65 PSV.dial PSR @17:11:05.02 Pulling 0.5ml of PSR.wash @17:11:05.03 PSR.select :wash @17:11:05.15 PS.reconfigure :default @17:11:05.19 PS.seek 0.5ml @17:11:11.19 delay 5 seconds @17:11:11.40 Stroke completed -- waiting for pressure to equalize ... @17:11:11.41 SS.reconfigure :exhale @17:11:11.45 SS.hold @17:11:16.26 PSR.select :air @17:11:16.37 PS.seek 2ml @17:11:25.83 Intake.select :close @17:11:26.49 SSV.dial :exhaust @17:11:27.26 Exhaust.select :open @17:11:27.37 SS.seek 0ml @17:11:32.47 PTV.dial :puck @17:11:33.23 PSV.dial PTV @17:11:33.97 PBV.dial :waste1 @17:11:34.40 PS.seek 1.5ml @17:11:36.16 Exhaust.select :close @17:11:36.27 SSV.dial CBV @17:11:36.30 CBV.dial SSV @17:11:37.06 Allowing 130s for 25.0ml of remaining 800.0ml @17:11:37.07 Intake.select :open @17:11:39.23 Intake @15.7psia, vacuum from 10.0 to 13.0psi @17:11:39.24 SS.reconfigure :inhaling 0.78ml/s @17:11:39.29 SS.seek 25ml @17:11:40.34 delay 3 seconds @17:11:43.36 PBV.dial between PSV and waste1 @17:11:43.74 PS.seek 0ml @17:12:00.06 delay 2:00 @17:12:00.95 Vacuum too high -- waiting up to 40 seconds @17:12:00.97 SS.reconfigure :exhale @17:12:01.01 SS.hold @17:12:07.54 Intake @14.7psia, vacuum from 9.2 to 12.2psi @17:12:07.56 SS.reconfigure :inhaling 0.70ml/s @17:12:07.60 SS.seek 25ml @17:12:22.32 Stroke completed -- waiting for pressure to equalize ... @17:12:22.33 SS.reconfigure :exhale @17:12:22.38 SS.hold @17:12:38.61 Intake.select :close @17:12:38.75 SSV.dial :exhaust @17:12:39.51 Exhaust.select :open @17:12:39.62 SS.seek 0ml @17:12:48.51 Exhaust.select :close @17:12:48.61 SSV.dial CBV @17:12:48.64 CBV.dial SSV @17:12:49.40 Allowing 130s for 25.0ml of remaining 775.0ml @17:12:49.41 Intake.select :open @17:12:51.57 Intake @15.6psia, vacuum from 10.0 to 13.0psi @17:12:51.58 SS.reconfigure :inhaling 0.70ml/s @17:12:51.63 SS.seek 25ml @17:13:17.48 Vacuum too high -- waiting up to 40 seconds @17:13:17.49 SS.reconfigure :exhale @17:13:17.54 SS.hold @17:13:26.10 Intake @15.1psia, vacuum from 9.6 to 12.6psi @17:13:26.12 SS.reconfigure :inhaling 0.63ml/s @17:13:26.16 SS.seek 25ml @17:13:39.96 Stroke completed -- waiting for pressure to equalize ... @17:13:39.97 SS.reconfigure :exhale @17:13:40.01 SS.hold @17:13:54.56 Intake.select :close @17:13:54.70 SSV.dial :exhaust @17:13:55.46 Exhaust.select :open @17:13:55.57 SS.seek 0ml @17:14:00.08 recoverPSRreagent08 @17:14:00.09 PTV.dial :puck @17:14:00.10 PBV.dial :waste1 @17:14:00.47 delay 30 seconds @17:14:04.36 Exhaust.select :close @17:14:04.47 SSV.dial CBV @17:14:04.49 CBV.dial SSV @17:14:05.27 Allowing 130s for 25.0ml of remaining 750.0ml @17:14:05.29 Intake.select :open @17:14:07.44 Intake @15.6psia, vacuum from 10.0 to 13.0psi @17:14:07.46 SS.reconfigure :inhaling 0.63ml/s @17:14:07.50 SS.seek 25ml @17:14:30.49 PBV.dial between PSV and waste1 @17:14:30.86 PSR.select :air @17:14:30.88 PSV.dial PSR @17:14:31.64 PS.reconfigure :fast @17:14:31.69 PS.seek 2.5ml @17:14:39.26 Vacuum too high -- waiting up to 40 seconds @17:14:39.28 SS.reconfigure :exhale @17:14:39.32 SS.hold @17:14:47.88 Intake @14.8psia, vacuum from 9.3 to 12.3psi @17:14:47.89 SS.reconfigure :inhaling 0.57ml/s @17:14:47.94 SS.seek 25ml @17:14:55.62 PSV.dial PTV @17:14:56.43 delay 5 seconds @17:14:59.60 Stroke completed -- waiting for pressure to equalize ... @17:14:59.61 SS.reconfigure :exhale @17:14:59.65 SS.hold @17:15:01.45 PSV.dial PSR @17:15:02.20 delay 5 seconds @17:15:07.21 PSV.dial PTV @17:15:07.98 delay 5 seconds @17:15:13.00 PSV.dial :air @17:15:13.94 PS.seek 0ml @17:15:15.89 Intake.select :close @17:15:16.04 SSV.dial :exhaust @17:15:16.80 Exhaust.select :open @17:15:16.91 SS.seek 0ml @17:15:25.91 Exhaust.select :close @17:15:26.02 SSV.dial CBV @17:15:26.05 CBV.dial SSV @17:15:26.79 Allowing 130s for 25.0ml of remaining 725.0ml @17:15:26.80 Intake.select :open @17:15:28.95 Intake @15.5psia, vacuum from 10.0 to 13.0psi @17:15:28.97 SS.reconfigure :inhaling 0.57ml/s @17:15:29.01 SS.seek 25ml @17:15:37.47 washCycleDA #2 of 2 @17:15:37.48 PSR.select :air @17:15:37.49 PSV.dial PSR @17:15:38.88 Pulling 0.5ml of PSR.wash @17:15:38.89 PSR.select :wash @17:15:39.00 PS.reconfigure :default @17:15:39.04 PS.seek 0.5ml @17:15:44.97 delay 5 seconds @17:15:49.98 PSR.select :air @17:15:50.60 PS.seek 2ml @17:16:06.80 PTV.dial :puck @17:16:06.82 PSV.dial PTV @17:16:07.70 PBV.dial :waste1 @17:16:08.13 PS.seek 1.5ml @17:16:12.67 Vacuum too high -- waiting up to 40 seconds @17:16:12.68 SS.reconfigure :exhale @17:16:12.72 SS.hold @17:16:14.02 delay 3 seconds @17:16:17.04 PBV.dial between PSV and waste1 @17:16:17.42 PS.seek 0ml @17:16:23.90 Intake @14.9psia, vacuum from 9.4 to 12.4psi @17:16:23.92 SS.reconfigure :inhaling 0.51ml/s @17:16:23.96 SS.seek 25ml @17:16:26.42 Stroke completed -- waiting for pressure to equalize ... @17:16:26.43 SS.reconfigure :exhale @17:16:26.48 SS.hold @17:16:33.83 delay 2:00 @17:16:37.51 Intake.select :close @17:16:37.65 SSV.dial :exhaust @17:16:38.39 Exhaust.select :open @17:16:38.50 SS.seek 0ml @17:16:47.52 Exhaust.select :close @17:16:47.63 SSV.dial CBV @17:16:47.64 CBV.dial SSV @17:16:48.39 Allowing 130s for 25.0ml of remaining 700.0ml @17:16:48.41 Intake.select :open @17:16:50.56 Intake @15.4psia, vacuum from 9.9 to 12.9psi @17:16:50.58 SS.reconfigure :inhaling 0.51ml/s @17:16:50.62 SS.seek 25ml @17:17:41.51 Stroke completed -- waiting for pressure to equalize ... @17:17:41.53 SS.reconfigure :exhale @17:17:41.57 SS.hold @17:18:04.04 Intake.select :close @17:18:04.18 SSV.dial :exhaust @17:18:04.92 Exhaust.select :open @17:18:05.03 SS.seek 0ml @17:18:13.90 Exhaust.select :close @17:18:14.01 SSV.dial CBV @17:18:14.04 CBV.dial SSV @17:18:14.78 Allowing 130s for 25.0ml of remaining 675.0ml @17:18:14.80 Intake.select :open @17:18:16.96 Intake @15.5psia, vacuum from 10.0 to 13.0psi @17:18:16.97 SS.reconfigure :inhaling 0.51ml/s @17:18:17.02 SS.seek 25ml @17:18:33.86 recoverPSRreagent08 @17:18:33.87 PTV.dial :puck @17:18:33.88 PBV.dial :waste1 @17:18:34.66 delay 30 seconds @17:19:00.29 Vacuum too high -- waiting up to 40 seconds @17:19:00.30 SS.reconfigure :exhale @17:19:00.34 SS.hold @17:19:04.77 PBV.dial between PSV and waste1 @17:19:05.19 PSR.select :air @17:19:05.22 PSV.dial PSR @17:19:05.97 PS.reconfigure :fast @17:19:06.02 PS.seek 2.5ml @17:19:13.02 Intake @15.2psia, vacuum from 9.7 to 12.7psi @17:19:13.03 SS.reconfigure :inhaling 0.47ml/s @17:19:13.08 SS.seek 25ml @17:19:21.86 Stroke completed -- waiting for pressure to equalize ... @17:19:21.87 SS.reconfigure :exhale @17:19:21.91 SS.hold @17:19:29.77 PSV.dial PTV @17:19:30.54 delay 5 seconds @17:19:35.55 PSV.dial PSR @17:19:36.30 delay 5 seconds @17:19:38.11 Intake.select :close @17:19:38.26 SSV.dial :exhaust @17:19:39.01 Exhaust.select :open @17:19:39.12 SS.seek 0ml @17:19:41.32 PSV.dial PTV @17:19:42.08 delay 5 seconds @17:19:47.10 PSV.dial :air @17:19:47.87 PS.seek 0ml @17:19:48.15 Exhaust.select :close @17:19:48.26 SSV.dial CBV @17:19:48.28 CBV.dial SSV @17:19:49.05 Allowing 130s for 25.0ml of remaining 650.0ml @17:19:49.06 Intake.select :open @17:19:51.21 Intake @15.5psia, vacuum from 10.0 to 13.0psi @17:19:51.23 SS.reconfigure :inhaling 0.47ml/s @17:19:51.27 SS.seek 25ml @17:20:11.33 getPMLReagent08 @17:20:11.35 PRV.dial :air @17:20:11.79 delay 5 seconds @17:20:16.82 PTV.dial :PRVmixing @17:20:18.15 delay 5 seconds @17:20:23.17 PRV.dial :ab2 @17:20:23.63 PTV.dial :PRVmixing @17:20:23.64 PSV.dial :mixloop @17:20:24.12 PS.reconfigure :default @17:20:24.16 PS.seek 0.3ml @17:20:28.11 delay 2 seconds @17:20:30.13 PRV.dial :air @17:20:30.66 PS.seek 0.55ml @17:20:34.15 addPMLReagent08 @17:20:34.16 PSV.dial :air @17:20:34.78 PS.seek 2ml @17:20:40.98 Vacuum too high -- waiting up to 40 seconds @17:20:40.99 SS.reconfigure :exhale @17:20:41.03 SS.hold @17:20:50.39 PSV.dial :PML @17:20:51.35 PTV.dial :mixing @17:20:52.10 PBV.dial :waste1 @17:20:52.53 PS.seek 1.85ml @17:20:54.95 PBV.dial between PSV and waste1 @17:20:55.34 PS.seek 0ml @17:20:55.71 Intake @15.7psia, vacuum from 10.0 to 13.0psi @17:20:55.73 SS.reconfigure :inhaling 0.43ml/s @17:20:55.77 SS.seek 25ml @17:21:02.56 Stroke completed -- waiting for pressure to equalize ... @17:21:02.57 SS.reconfigure :exhale @17:21:02.61 SS.hold @17:21:15.24 delay 2 seconds @17:21:16.89 Intake.select :close @17:21:17.03 SSV.dial :exhaust @17:21:17.25 delay 8:00 @17:21:17.78 Exhaust.select :open @17:21:17.89 SS.seek 0ml @17:21:27.17 Exhaust.select :close @17:21:27.28 SSV.dial CBV @17:21:27.30 CBV.dial SSV @17:21:28.06 Allowing 130s for 25.0ml of remaining 625.0ml @17:21:28.07 Intake.select :open @17:21:30.23 Intake @15.5psia, vacuum from 10.0 to 13.0psi @17:21:30.25 SS.reconfigure :inhaling 0.43ml/s @17:21:30.29 SS.seek 25ml @17:22:31.32 Stroke completed -- waiting for pressure to equalize ... @17:22:31.33 SS.reconfigure :exhale @17:22:31.38 SS.hold @17:23:01.88 Intake.select :close @17:23:02.02 SSV.dial :exhaust @17:23:02.76 Exhaust.select :open @17:23:02.87 SS.seek 0ml @17:23:11.90 Exhaust.select :close @17:23:12.01 SSV.dial CBV @17:23:12.04 CBV.dial SSV @17:23:12.78 Allowing 130s for 25.0ml of remaining 600.0ml @17:23:12.79 Intake.select :open @17:23:14.94 Intake @15.5psia, vacuum from 10.0 to 13.0psi @17:23:14.96 SS.reconfigure :inhaling 0.43ml/s @17:23:15.00 SS.seek 25ml @17:24:07.25 Vacuum too high -- waiting up to 40 seconds @17:24:07.26 SS.reconfigure :exhale @17:24:07.30 SS.hold @17:24:23.61 Intake @15.6psia, vacuum from 10.0 to 13.0psi @17:24:23.63 SS.reconfigure :inhaling 0.38ml/s @17:24:23.67 SS.seek 25ml @17:24:34.72 Stroke completed -- waiting for pressure to equalize ... @17:24:34.74 SS.reconfigure :exhale @17:24:34.78 SS.hold @17:24:53.02 Intake.select :close @17:24:53.16 SSV.dial :exhaust @17:24:53.91 Exhaust.select :open @17:24:54.02 SS.seek 0ml @17:25:02.88 Exhaust.select :close @17:25:02.99 SSV.dial CBV @17:25:03.01 CBV.dial SSV @17:25:03.77 Allowing 130s for 25.0ml of remaining 575.0ml @17:25:03.79 Intake.select :open @17:25:05.94 Intake @15.6psia, vacuum from 10.0 to 13.0psi @17:25:05.96 SS.reconfigure :inhaling 0.38ml/s @17:25:06.00 SS.seek 25ml @17:26:09.81 Vacuum too high -- waiting up to 40 seconds @17:26:09.82 SS.reconfigure :exhale @17:26:09.86 SS.hold @17:26:26.16 Intake @15.7psia, vacuum from 10.0 to 13.0psi @17:26:26.18 SS.reconfigure :inhaling 0.34ml/s @17:26:26.23 SS.seek 25ml @17:26:33.52 Stroke completed -- waiting for pressure to equalize ... @17:26:33.53 SS.reconfigure :exhale @17:26:33.58 SS.hold @17:26:51.83 Intake.select :close @17:26:51.97 SSV.dial :exhaust @17:26:52.73 Exhaust.select :open @17:26:52.84 SS.seek 0ml @17:27:01.72 Exhaust.select :close @17:27:01.83 SSV.dial CBV @17:27:01.85 CBV.dial SSV @17:27:03.16 Allowing 130s for 25.0ml of remaining 550.0ml @17:27:03.17 Intake.select :open @17:27:05.33 Intake @15.8psia, vacuum from 10.0 to 13.0psi @17:27:05.34 SS.reconfigure :inhaling 0.34ml/s @17:27:05.39 SS.seek 25ml @17:28:19.56 Vacuum too high -- waiting up to 40 seconds @17:28:19.57 SS.reconfigure :exhale @17:28:19.61 SS.hold @17:28:34.01 SS.reconfigure :inhaling 0.31ml/s @17:28:34.05 SS.seek 25ml @17:28:43.55 Stroke completed -- waiting for pressure to equalize ... @17:28:43.56 SS.reconfigure :exhale @17:28:43.60 SS.hold @17:29:01.93 Intake.select :close @17:29:02.07 SSV.dial :exhaust @17:29:02.83 Exhaust.select :open @17:29:02.94 SS.seek 0ml @17:29:11.78 Exhaust.select :close @17:29:11.89 SSV.dial CBV @17:29:11.91 CBV.dial SSV @17:29:12.69 Allowing 130s for 25.0ml of remaining 525.0ml @17:29:12.70 Intake.select :open @17:29:14.86 Intake @15.6psia, vacuum from 10.0 to 13.0psi @17:29:14.88 SS.reconfigure :inhaling 0.31ml/s @17:29:14.92 SS.seek 25ml @17:29:17.27 recoverPRVreagent08 @17:29:17.29 PTV.dial :mixing @17:29:17.30 PBV.dial :waste1 @17:29:17.67 delay 30 seconds @17:29:47.69 PBV.dial between PSV and waste1 @17:29:48.06 PSV.dial :air @17:29:48.53 PS.reconfigure :fast @17:29:48.58 PS.seek 2.5ml @17:30:12.38 PSV.dial :mixloop @17:30:12.85 delay 2 seconds @17:30:14.87 PSV.dial :air @17:30:15.34 delay 2 seconds @17:30:17.35 PSV.dial :mixloop @17:30:17.82 delay 2 seconds @17:30:19.84 PSV.dial :air @17:30:20.31 PS.seek 0ml @17:30:43.73 washCycleDA #1 of 3 @17:30:43.74 PSR.select :air @17:30:43.75 PSV.dial PSR @17:30:45.12 Pulling 0.5ml of PSR.wash @17:30:45.13 PSR.select :wash @17:30:45.25 PS.reconfigure :default @17:30:45.29 PS.seek 0.5ml @17:30:47.72 Stroke completed -- waiting for pressure to equalize ... @17:30:47.73 SS.reconfigure :exhale @17:30:47.77 SS.hold @17:30:51.32 delay 5 seconds @17:30:56.33 PSR.select :air @17:30:56.45 PS.seek 2ml @17:31:12.57 PTV.dial :puck @17:31:13.30 PSV.dial PTV @17:31:14.05 PBV.dial :waste1 @17:31:14.49 PS.seek 1.5ml @17:31:20.39 Intake.select :close @17:31:20.45 delay 3 seconds @17:31:20.54 SSV.dial :exhaust @17:31:21.31 Exhaust.select :open @17:31:21.42 SS.seek 0ml @17:31:23.47 PBV.dial between PSV and waste1 @17:31:23.86 PS.seek 0ml @17:31:30.51 Exhaust.select :close @17:31:30.62 SSV.dial CBV @17:31:30.64 CBV.dial SSV @17:31:31.42 Allowing 130s for 25.0ml of remaining 500.0ml @17:31:31.44 Intake.select :open @17:31:33.59 Intake @15.7psia, vacuum from 10.0 to 13.0psi @17:31:33.61 SS.reconfigure :inhaling 0.31ml/s @17:31:33.65 SS.seek 25ml @17:31:40.04 delay 2:00 @17:33:05.10 Stroke completed -- waiting for pressure to equalize ... @17:33:05.12 SS.reconfigure :exhale @17:33:05.16 SS.hold @17:33:40.06 recoverPSRreagent08 @17:33:40.07 PTV.dial :puck @17:33:40.08 PBV.dial :waste1 @17:33:40.44 delay 30 seconds @17:33:41.45 SS.reconfigure :default @17:33:41.49 SS.hold @17:33:41.60 Intake.select :close @17:33:41.75 ShallowSampler::Clogged in WCR -- Filtering too Slowly after 525.0ml @17:33:41.76 SSV.dial :exhaust @17:33:42.51 Exhaust.select :open @17:33:42.62 SS.reconfigure :exhale @17:33:42.67 SS.seek 0ml @17:33:51.53 Exhaust.select :close @17:33:51.64 Sampled 525.0ml @17:33:51.65 Drying puck before fixing for 20:00 @17:33:51.66 CBV.dial :waste2b @17:33:52.43 delay 1:00 @17:34:10.47 PBV.dial between PSV and waste1 @17:34:10.83 PSR.select :air @17:34:10.85 PSV.dial PSR @17:34:11.62 PS.reconfigure :fast @17:34:11.66 PS.seek 2.5ml @17:34:35.47 PSV.dial PTV @17:34:36.24 delay 5 seconds @17:34:41.26 PSV.dial PSR @17:34:42.00 delay 5 seconds @17:34:47.03 PSV.dial PTV @17:34:47.89 delay 5 seconds @17:34:52.45 CSV.dial :air @17:34:52.90 CS.reconfigure :fast @17:34:52.95 CS.seek 10ml @17:34:52.99 PSV.dial :air @17:34:53.88 PS.seek 0ml @17:35:17.13 Pumping wcr puck from tube 3 up to 35psi... @17:35:17.15 CBV.dial SSV @17:35:17.29 washCycleDA #2 of 3 @17:35:17.30 PSR.select :air @17:35:17.31 PSV.dial PSR @17:35:18.46 CBV.dial between CSV and waste2b @17:35:19.10 Pulling 0.5ml of PSR.wash @17:35:19.11 PSR.select :wash @17:35:19.22 PS.reconfigure :default @17:35:19.26 PS.seek 0.5ml @17:35:19.94 CSV.dial between air and CTV @17:35:20.27 CS.seek 7.232ml @17:35:25.13 delay 5 seconds @17:35:27.36 CTV.dial CSV,avoiding: :air @17:35:27.38 CSV.dial CTV @17:35:28.18 CS.reconfigure :pressurize @17:35:28.23 CS.seek 0ml @17:35:30.15 PSR.select :air @17:35:30.26 PS.seek 2ml @17:35:46.37 CBV.dial :waste2b @17:35:46.43 PTV.dial :puck @17:35:46.45 PSV.dial PTV @17:35:46.71 CS.reconfigure :default @17:35:46.76 CS.seek 0ml @17:35:47.20 PBV.dial :waste1 @17:35:47.64 PS.seek 1.5ml @17:35:48.26 delay 1:00 @17:35:53.66 delay 3 seconds @17:35:56.68 PBV.dial between PSV and waste1 @17:35:57.04 PS.seek 0ml @17:36:13.38 delay 2:00 @17:36:48.28 CTV.dial :air,avoiding: :intake @17:36:49.62 Fix #1 of 2 (with RNAlater) for 10:00 @17:36:49.63 CSV.dial CSR @17:36:50.12 Pulling 2ml of CSR.RNAlater @17:36:50.13 CSR.select :RNAlater @17:36:50.25 CS.seek 2ml @17:36:56.16 delay 5 seconds @17:37:01.18 CSR.select :air @17:37:01.29 CS.reconfigure :fast @17:37:01.34 CS.seek 10ml @17:37:20.72 Pumping wcr puck from tube 3 up to 35psi... @17:37:20.74 CBV.dial SSV @17:37:22.04 CBV.dial between CSV and waste2b @17:37:23.00 CSV.dial between air and CTV @17:37:23.61 CS.seek 7.181ml @17:37:30.79 CTV.dial CSV,avoiding: :air @17:37:30.81 CSV.dial CTV @17:37:32.20 CS.reconfigure :pressurize @17:37:32.24 CS.seek 0ml @17:37:46.71 delay 10:00 @17:38:13.40 recoverPSRreagent08 @17:38:13.41 PTV.dial :puck @17:38:13.42 PBV.dial :waste1 @17:38:13.78 delay 30 seconds @17:38:43.81 PBV.dial between PSV and waste1 @17:38:44.16 PSR.select :air @17:38:44.18 PSV.dial PSR @17:38:44.94 PS.reconfigure :fast @17:38:44.99 PS.seek 2.5ml @17:39:08.68 PSV.dial PTV @17:39:09.45 delay 5 seconds @17:39:14.47 PSV.dial PSR @17:39:15.22 delay 5 seconds @17:39:20.24 PSV.dial PTV @17:39:21.11 delay 5 seconds @17:39:26.13 PSV.dial :air @17:39:26.90 PS.seek 0ml @17:39:50.22 washCycleDA #3 of 3 @17:39:50.23 PSR.select :air @17:39:50.24 PSV.dial PSR @17:39:51.61 Pulling 0.5ml of PSR.wash @17:39:51.62 PSR.select :wash @17:39:51.73 PS.reconfigure :default @17:39:51.78 PS.seek 0.5ml @17:39:57.69 delay 5 seconds @17:40:02.70 PSR.select :air @17:40:02.82 PS.seek 2ml @17:40:18.87 PTV.dial :puck @17:40:18.88 PSV.dial PTV @17:40:19.61 PBV.dial :waste1 @17:40:20.02 PS.seek 1.5ml @17:40:26.03 delay 3 seconds @17:40:29.04 PBV.dial between PSV and waste1 @17:40:29.41 PS.seek 0ml @17:40:45.53 delay 2:00 @17:42:45.56 recoverPSRreagent08 PTV.dial :puck @17:42:45.57 PBV.dial :waste1 @17:42:45.95 delay 30 seconds @17:43:15.97 PBV.dial between PSV and waste1 @17:43:16.34 PSR.select :air @17:43:16.36 PSV.dial PSR @17:43:17.59 PS.reconfigure :fast @17:43:17.64 PS.seek 2.5ml @17:43:41.30 PSV.dial PTV @17:43:42.07 delay 5 seconds @17:43:47.09 PSV.dial PSR @17:43:47.84 delay 5 seconds @17:43:52.86 PSV.dial PTV @17:43:53.73 delay 5 seconds @17:43:58.75 PSV.dial :air @17:43:59.64 PS.seek 0ml @17:44:22.74 Gate.power :camera,:ON @17:44:22.75 Mixing DA Substrates @17:44:22.76 PSR.select :air @17:44:22.78 PSV.dial PSR @17:44:24.32 Pulling 0.25ml of PSR.sub1 @17:44:24.35 PSR.select :sub1 @17:44:24.58 PS.reconfigure :default @17:44:24.67 PS.seek 0.25ml @17:44:28.09 delay 5 seconds @17:44:33.17 Pulling 0.25ml of PSR.sub2 @17:44:33.18 PSR.select :sub2 @17:44:33.29 PS.seek 0.5ml @17:44:36.78 delay 5 seconds @17:44:41.79 PSR.select :air @17:44:41.90 PS.seek 2ml @17:44:58.02 PTV.dial :puck @17:44:58.03 PSV.dial PTV @17:44:58.79 PBV.dial :waste1 @17:44:59.21 PS.seek 1.5ml @17:45:05.08 delay 3 seconds @17:45:08.10 PBV.dial between PSV and waste1 @17:45:08.48 PS.seek 0ml @17:45:24.65 delay 10 seconds @17:45:34.67 recoverPSRreagent08 @17:45:34.68 PTV.dial :puck @17:45:34.69 PBV.dial :waste1 @17:45:35.07 delay 30 seconds @17:46:05.09 PBV.dial between PSV and waste1 @17:46:05.46 PSR.select :air @17:46:05.48 PSV.dial PSR @17:46:06.25 PS.reconfigure :fast @17:46:06.29 PS.seek 2.5ml @17:46:30.27 PSV.dial PTV @17:46:31.04 delay 5 seconds @17:46:36.06 PSV.dial PSR @17:46:36.82 delay 5 seconds @17:46:41.84 PSV.dial PTV @17:46:42.60 delay 5 seconds @17:46:47.62 PSV.dial :air @17:46:48.39 PS.seek 0ml @17:47:11.99 Additional air clear @17:47:12.00 PSR.select :air @17:47:12.02 PSV.dial PSR @17:47:13.40 Pulling 2ml of PSR.air @17:47:13.41 PSR.select :air @17:47:13.44 PS.reconfigure :default @17:47:13.48 PS.seek 2ml @17:47:35.02 delay 5 seconds @17:47:40.04 PTV.dial :puck @17:47:40.05 PSV.dial PTV @17:47:40.80 PBV.dial :waste1 @17:47:41.24 PS.seek 1.5ml @17:47:46.73 CBV.dial :waste2b @17:47:47.08 CS.reconfigure :default @17:47:47.15 CS.seek 0ml @17:47:47.17 delay 3 seconds @17:47:50.19 PBV.dial between PSV and waste1 @17:47:50.57 delay 1 second @17:47:51.59 PSV.dial :waste1 @17:47:52.25 delay 1:00 @17:47:52.65 PS.seek 0ml @17:48:08.85 Processing Puck Heater at 28C before imaging @17:48:08.86 PH.coast @17:48:08.98 Imaging da puck in Processing Clamp while storing it to tube 2 @17:48:09.14 SC.reconfigure :tight @17:48:09.19 SC.seek 2 @17:48:12.71 SC.configure :limp @17:48:12.91 Elbow.reconfigure :tight @17:48:12.96 Elbow.seek 2 @17:48:14.74 Forearm.reconfigure :tight @17:48:14.78 Forearm.seek 2 + 450 counts @17:48:15.92 SE.reconfigure :pick @17:48:15.97 SE.seek :beamBreak @17:48:17.99 SE.configure :default @17:48:18.14 Elbow.reconfigure :limp @17:48:18.18 Forearm.reconfigure :loose @17:48:18.22 Forearm.seek 2 @17:48:18.52 Elbow.hold @17:48:18.58 Elbow.reconfigure :tight @17:48:18.67 SE.seek 16.11 Pucks below Top Plate @17:48:19.21 Forearm.force -20 @17:48:19.25 Forearm.seek 2 + 288 counts @17:48:19.52 Forearm.reconfigure :tight @17:48:19.57 Forearm.seek 2 + 450 counts @17:48:19.78 SE.seek 17.11 Pucks below Top Plate @17:48:20.36 Forearm.reconfigure :loose @17:48:20.41 Forearm.seek :retract @17:48:21.86 Elbow.seek PC @17:48:24.54 Elbow.reconfigure :loose @17:48:24.63 Forearm.seek PC @17:48:25.59 Hand.close @17:48:26.16 PC.seek :open @17:48:30.94 Forearm.seek :retract @17:48:31.94 Elbow.reconfigure :tight @17:48:31.98 Elbow.seek 2 @17:48:34.55 Forearm.reconfigure :tight @17:48:34.60 Forearm.seek 2 + 450 counts @17:48:35.53 Hand.open @17:48:36.22 SE.seek 16.76 Pucks below Top Plate @17:48:36.48 SE.reconfigure :seal @17:48:36.53 SE.seek 15.8 Pucks below Top Plate @17:48:37.09 Lights, camera, ACTION! @17:48:37.12 PuckCamera: snap -bin=2,2 -auto /var/log/esp/da23jan2617h475ml.tif @17:48:52.27 CTV.dial :air,avoiding: :intake @17:48:53.64 Fix #2 of 2 (with RNAlater) for 10:00 @17:48:53.66 CSV.dial CSR @17:48:54.15 Pulling 2ml of CSR.RNAlater @17:48:54.17 CSR.select :RNAlater @17:48:54.28 CS.seek 2ml @17:48:56.58 Waiting >20 seconds for da to relinquish FlushPuck @17:48:59.23 SX HX9/16 (USB:13): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 18.81 seconds /var/log/esp/da23jan2617h475ml.tif: TIFF Upload Complete @17:48:59.25 PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da23jan2617h475ml15s.tif @17:49:00.31 delay 5 seconds @17:49:05.33 CSR.select :air @17:49:05.44 CS.reconfigure :fast @17:49:05.49 CS.seek 10ml @17:49:14.87 SX HX9/16 (USB:13): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15 seconds /var/log/esp/midres/da23jan2617h475ml15s.tif: TIFF Upload Complete @17:49:14.89 PuckCamera: snap -bin=2,2 20.0 /var/log/esp/midres/da23jan2617h475ml20s.tif @17:49:25.50 Pumping wcr puck from tube 3 up to 35psi... @17:49:25.52 CBV.dial SSV @17:49:26.84 CBV.dial between CSV and waste2b @17:49:27.82 CSV.dial between air and CTV @17:49:28.42 CS.seek 7.144ml @17:49:35.55 SX HX9/16 (USB:13): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 20 seconds /var/log/esp/midres/da23jan2617h475ml20s.tif: TIFF Upload Complete @17:49:35.57 Gate.power :camera,:OFF @17:49:35.58 DAfans.email "18.81 sec auto-exposure",Subject: "da23jan2617h475ml images!" @17:49:35.84 Elbow.seek 2 @17:49:35.93 CTV.dial CSV,avoiding: :air @17:49:35.99 CSV.dial CTV @17:49:36.19 Forearm.seek 2 + 450 counts @17:49:36.28 SE.reconfigure :gently @17:49:36.33 SE.seek 16.52 Pucks below Top Plate @17:49:36.98 Elbow.reconfigure :limp @17:49:37.04 Forearm.reconfigure :loose @17:49:37.09 Forearm.seek 2 @17:49:37.35 Elbow.hold @17:49:37.46 CS.reconfigure :pressurize @17:49:37.47 Elbow.reconfigure :tight @17:49:37.60 CS.seek 0ml @17:49:37.63 SE.reconfigure :default @17:49:37.72 SE.seek 16.7 Pucks below Top Plate @17:49:38.09 Forearm.force -20 @17:49:38.13 Forearm.seek 2 + 286 counts @17:49:38.43 Forearm.reconfigure :tight @17:49:38.48 Forearm.seek 2 + 450 counts @17:49:38.74 SE.seek :down @17:49:40.54 Forearm.reconfigure :loose @17:49:40.63 Forearm.seek :retract @17:49:40.69 SE.stop @17:49:41.78 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @17:49:42.15 Elbow.seek FlushPuck::Garage @17:49:44.06 Elbow.reconfigure :loose @17:49:44.24 Forearm.seek :garage @17:49:45.29 Hand.close @17:49:46.23 Forearm.seek :retract @17:49:47.24 Elbow.reconfigure :tight @17:49:47.42 Elbow.seek PC @17:49:48.76 Elbow.reconfigure :loose @17:49:48.95 Forearm.reconfigure :tight @17:49:49.04 Forearm.seek PC @17:49:50.64 PC.seek :closed @17:49:52.16 delay 10:00 @17:49:55.46 Processing Clamp onSpring/offSpring for 16/26 samples, on/off torque 1.60 @17:49:55.66 Hand.open @17:49:56.42 Forearm.reconfigure :loose @17:49:56.50 Forearm.seek :retract @17:49:57.48 PSR.select :air @17:49:57.52 PSV.dial PSR @17:49:58.15 Pulling 0.5ml of PSR.flush @17:49:58.17 PSR.select :flush @17:49:58.34 PS.seek 0.5ml @17:50:04.29 delay 5 seconds @17:50:09.32 PSR.select :air @17:50:09.47 PS.seek 2.5ml @17:50:11.69 upsync @17:50:30.80 delay 1 second @17:50:31.81 PRV.dial :waste2,avoiding: :ab2 @17:50:33.69 PTV.dial :PRVmixing @17:50:35.00 PSV.dial :mixloop @17:50:36.16 PS.seek :empty @17:51:02.54 PSR.select :air @17:51:02.56 PSV.dial PSR @17:51:03.66 Pulling 0.5ml of PSR.flush @17:51:03.67 PSR.select :flush @17:51:03.79 PS.seek 0.5ml @17:51:09.83 delay 5 seconds @17:51:14.85 PSR.select :air @17:51:14.96 PS.seek 2.5ml @17:51:36.26 delay 1 second @17:51:37.28 PBV.dial :waste1 @17:51:37.66 PTV.dial :mixing @17:51:38.40 PSV.dial :mixloop @17:51:39.47 PS.seek :empty @17:52:05.83 PSV.dial PSR @17:52:06.87 PSR.select :flush @17:52:06.99 PS.seek 1ml @17:52:18.32 PSR.select :air @17:52:18.44 PS.seek 2.5ml @17:52:34.61 PSV.dial PTV @17:52:35.37 PTV.dial :puck @17:52:36.11 PBV.dial :waste1 @17:52:36.13 PS.seek 0ml @17:53:02.70 PSV.dial PSR @17:53:03.57 PSR.select :flush @17:53:03.69 PS.seek 0.5ml @17:53:09.82 PSR.select :air @17:53:09.93 PS.seek 2.5ml @17:53:31.26 PSV.dial PTV @17:53:32.00 PBV.dial PSV @17:53:32.56 PS.seek 0ml @17:53:59.10 PSV.dial PBV @17:53:59.58 PS.seek 2.5ml @17:54:25.88 PSV.dial :waste1 @17:54:26.63 PS.seek 0ml @17:54:53.01 PSV.dial PSR @17:54:53.48 PSR.select :air @17:54:53.50 PS.seek 2.5ml @17:55:20.01 PSV.dial PTV @17:55:20.87 PBV.dial PSV @17:55:20.89 PS.seek 0ml @17:55:47.41 PSV.dial PBV @17:55:47.87 PS.seek 2.5ml @17:56:14.16 PSV.dial :waste1 @17:56:15.39 PS.seek 0ml @17:56:41.73 PSV.dial PSR @17:56:42.19 PSR.select :flush @17:56:42.31 PS.seek 1ml @17:56:53.38 PSR.select :air @17:56:53.50 PS.seek 2.5ml @17:57:09.73 PSV.dial PTV @17:57:10.46 PTV.dial :puck @17:57:10.47 PBV.dial :waste1 @17:57:11.03 PS.seek 0ml @17:57:37.38 PSV.dial PSR @17:57:38.13 PSR.select :flush @17:57:38.25 PS.seek 0.5ml @17:57:44.16 PSR.select :air @17:57:44.27 PS.seek 2.5ml @17:58:05.51 PSV.dial PTV @17:58:06.26 PBV.dial PSV @17:58:06.83 PS.seek 0ml @17:58:33.16 PSV.dial PBV @17:58:33.64 PS.seek 2.5ml @17:58:59.91 PSV.dial :waste1 @17:59:00.65 PS.seek 0ml @17:59:27.00 PSV.dial PSR @17:59:27.47 PSR.select :air @17:59:27.50 PS.seek 2.5ml @17:59:52.19 CBV.dial :waste2b @17:59:52.52 CS.reconfigure :default @17:59:52.56 CS.seek 0ml @17:59:53.82 PSV.dial PTV @17:59:54.55 PBV.dial PSV @17:59:54.57 PS.seek 0ml @17:59:57.76 delay 1:00 @18:00:20.88 PSV.dial PBV @18:00:21.35 PS.seek 2.5ml @18:00:47.88 PSV.dial :waste1 @18:00:48.74 PS.seek 0ml @18:00:57.79 CTV.dial :air,avoiding: :intake @18:00:59.16 Storing wcr puck in Collection Clamp to tube 2 @18:00:59.38 SC.reconfigure :tight @18:00:59.42 SC.seek 2 @18:00:59.65 SC.configure :limp @18:00:59.85 Elbow.reconfigure :tight @18:00:59.90 Elbow.seek 2 @18:01:02.55 Forearm.reconfigure :tight @18:01:02.59 Forearm.seek 2 + 450 counts @18:01:03.61 SE.reconfigure :pick @18:01:03.67 SE.seek :beamBreak @18:01:05.61 SE.configure :default @18:01:05.76 Elbow.reconfigure :limp @18:01:05.81 Forearm.reconfigure :loose @18:01:05.85 Forearm.seek 2 @18:01:06.19 Elbow.hold @18:01:06.26 Elbow.reconfigure :tight @18:01:06.35 SE.seek 17.11 Pucks below Top Plate @18:01:06.81 Forearm.force -20 @18:01:06.85 Forearm.seek 2 + 278 counts @18:01:07.14 Forearm.reconfigure :tight @18:01:07.18 Forearm.seek 2 + 450 counts @18:01:07.37 SE.seek 18.11 Pucks below Top Plate @18:01:08.03 Forearm.reconfigure :loose @18:01:08.07 Forearm.seek :retract @18:01:09.07 Elbow.seek CC @18:01:10.65 Elbow.reconfigure :loose @18:01:10.75 Forearm.seek CC @18:01:11.72 Hand.close @18:01:12.27 CC.seek :open @18:01:17.46 Forearm.seek :retract @18:01:18.48 Elbow.reconfigure :tight @18:01:18.52 Elbow.seek 2 @18:01:20.29 Elbow.reconfigure :limp @18:01:20.34 Forearm.seek 2 @18:01:21.30 Elbow.hold @18:01:21.37 Elbow.reconfigure :tight @18:01:21.42 Hand.open @18:01:22.14 Forearm.force -20 @18:01:22.18 Forearm.seek 2 + 303 counts @18:01:22.49 Forearm.reconfigure :tight @18:01:22.53 Forearm.seek 2 + 450 counts @18:01:22.79 SE.seek :down @18:01:23.92 Forearm.reconfigure :loose @18:01:23.96 Forearm.seek :retract @18:01:24.28 SE.stop @18:01:24.87
Starting finalPurge @18:01:24.89 Garaging FlushPuck in Processing Clamp @18:01:25.03 Elbow.seek PC @18:01:27.68 Elbow.reconfigure :loose @18:01:27.77 Forearm.seek PC @18:01:28.74 Hand.close @18:01:29.40 PC.seek :open @18:01:34.11 Forearm.seek :retract @18:01:35.05 Elbow.reconfigure :tight @18:01:35.10 Elbow.seek :garage @18:01:36.44 Elbow.reconfigure :loose @18:01:36.53 Forearm.reconfigure :tight @18:01:36.57 Forearm.seek :garage @18:01:37.52 Hand.open @18:01:38.20 Forearm.reconfigure :loose @18:01:38.25 Forearm.seek :retract @18:01:39.22
Loading FlushPuck into Collection Clamp from FlushPuck::Garage @18:01:39.34 Elbow.reconfigure :tight @18:01:39.38 Elbow.seek FlushPuck::Garage @18:01:39.56 Elbow.reconfigure :loose @18:01:39.65 Forearm.seek :garage @18:01:40.63 Hand.close @18:01:41.38 Forearm.seek :retract @18:01:42.37 Elbow.reconfigure :tight @18:01:42.41 Elbow.seek CC @18:01:45.26 Elbow.reconfigure :loose @18:01:45.35 Forearm.reconfigure :tight @18:01:45.39 Forearm.seek CC @18:01:46.67 CC.seek :closed @18:01:51.18 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.23 @18:01:51.25 Hand.open @18:01:51.95 Forearm.reconfigure :loose @18:01:52.00 Forearm.seek :retract @18:01:52.97 Flushing the Collection Valve::Manifold @18:01:53.10 CSV.dial CSR @18:01:53.58 Pulling 5ml of CSR.flush @18:01:53.59 CSR.select :flush @18:01:53.70 CS.seek 5ml @18:02:07.57 delay 5 seconds @18:02:12.59 CSR.select :air @18:02:12.74 CS.reconfigure :fast @18:02:12.79 CS.seek 7.5ml @18:02:19.54 CSV.dial :waste @18:02:20.81 CS.reconfigure :default @18:02:20.85 CS.seek 0ml @18:02:40.92 Flushing lower manifold's waste1 port @18:02:41.03 CSV.dial :flush @18:02:41.47 CS.reconfigure :fast @18:02:41.51 CS.seek 5ml @18:02:54.16 delay 5 seconds @18:02:59.18 CSV.dial :air @18:03:00.16 CS.seek 7ml @18:03:05.63 CSV.dial CTV @18:03:05.65
CTV.dial CSV @18:03:07.02 CBV.dial :waste1 @18:03:07.80 CS.seek 0ml @18:03:24.38 Flushing lower manifold's waste2 port @18:03:24.50 CSV.dial :flush @18:03:25.23 CS.seek 5ml @18:03:38.04 delay 5 seconds @18:03:43.06 CSV.dial :air @18:03:43.57 CS.seek 7ml @18:03:49.16 CSV.dial CTV @18:03:49.19
CTV.dial CSV @18:03:49.63 CBV.dial :waste2 @18:03:50.68 CS.seek 0ml @18:04:07.35 Flushing lower manifold's waste2b port @18:04:07.47 CSV.dial :flush @18:04:08.20 CS.seek 5ml @18:04:21.10 delay 5 seconds @18:04:26.12 CSV.dial :air @18:04:26.99 CS.seek 7ml @18:04:32.60 CSV.dial CTV @18:04:32.62
CTV.dial CSV @18:04:33.07 CBV.dial :waste2b @18:04:34.11 CS.seek 0ml @18:04:50.76 Disinfecting external Intake with kill @18:04:50.87 CSV.dial CSR @18:04:51.36 Pulling 8ml of CSR.kill @18:04:51.37 CSR.select :kill @18:04:51.48 CS.seek 8ml @18:05:11.45 delay 5 seconds @18:05:16.46 CSR.select :air @18:05:16.58 CS.seek 10ml @18:05:22.08 CSV.dial CBV @18:05:22.10
CBV.dial CSV @18:05:23.15 CTV.dial :intake @18:05:23.93 Intake.select :open @18:05:24.03 CS.seek 2ml @18:05:42.91 Intake.select :close @18:05:43.01 CTV.dial :air @18:05:43.77 CS.reconfigure :default @18:05:43.82 CS.seek 0ml @18:05:49.75 CSV.dial CSR @18:05:50.81 Pulling 5ml of CSR.flush @18:05:50.82 CSR.select :flush @18:05:50.94 CS.seek 5ml @18:06:04.73 delay 5 seconds @18:06:09.74 CSR.select :air @18:06:09.86 CS.seek 10ml @18:06:23.50 CSV.dial CBV @18:06:24.51 CS.seek 0ml @18:06:50.88 Garaging FlushPuck in Collection Clamp @18:06:50.95 Elbow.reconfigure :tight @18:06:50.99 Elbow.seek CC @18:06:51.35 Elbow.reconfigure :loose @18:06:51.44 Forearm.seek CC @18:06:52.44 Hand.close @18:06:53.02 CC.seek :open @18:06:57.64 Forearm.seek :retract @18:06:58.63 Elbow.reconfigure :tight @18:06:58.67 Elbow.seek :garage @18:07:01.58 Elbow.reconfigure :loose @18:07:01.68 Forearm.reconfigure :tight @18:07:01.72 Forearm.seek :garage @18:07:02.72 Hand.open @18:07:03.45 Forearm.reconfigure :loose @18:07:03.49 Forearm.seek :retract @18:07:04.45 Completed [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] begun 15:49:15 @18:07:04.50 Asleep after completing DA_WCR_12623 assays @18:07:04.52 GoodNews.email "{1 => 33,\n 2 => 19,\n 3 => 14,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@17:58:48, 17% flow, 18.6C, 32% humidity, 14.7psia, 13.715V, 0.496A, 0.484A avg, 6.80W\nSampled 525.0ml",Subject: "Asleep after completing DA_WCR_12623 assays" @18:07:09.74 upsync @13:52:56.74UTC30-Jan-23
ESP2 v3.41 I2C Gateway v3.20 for core bus @13:52:59.47 Configuration for short puck jake 1/11/21 by brent@mbari.org /home/esp/esp2/type/shallow/jake/configure.rb @13:53:03.61 Polling Can every 10:00 @13:53:03.76 Cleanly exited at 13:49:58UTC30-Jan-23 @13:53:03.80 Loading /home/esp/esp2/mission/DA_WCR_13023.rb mission @13:53:03.85 GoodNews.email "Starting DA_WCR_13023 w/pucks in tube 3\n{1 => 33,\n 2 => 19,\n 3 => 14,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@13:53:03, 17% flow, 16.8C, 34% humidity, 14.7psia, 13.759V, 0.315A, 0.302A avg, 4.33W",Subject: "DA_WCR_13023 begun" @13:53:03.89
Preparing to sleep until + 00:00:05 @13:53:03.91 Asleep til + 00:00:05 @13:53:03.93 Continuing at 13:53 @13:53:04.22 Gate.power :core,:OFF @13:53:04.23 Gate.power :raw,:OFF @13:53:04.24 Shutdown @13:53:08.83
Powering core bus @13:53:08.92 GoodNews.email "Then, in tube 3: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2/mission/DA_WCR_13023.rb:14\n{1 => 33,\n 2 => 19,\n 3 => 14,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@13:53:03, 17% flow, 16.8C, 34% humidity, 14.7psia, 13.759V, 0.315A, 0.302A avg, 4.33W",Subject: "Asleep until 13:53" @13:53:10.67 upsync @13:53:14.26
Gate.power :raw,:ON @13:53:14.82 upsync @13:53:15.20
CS.configure :default @13:53:15.28 PS.configure :default @13:53:15.37 SS.configure :default @13:53:15.46 CC.configure :default @13:53:15.55 PC.configure :default @13:53:15.66 Forearm.configure :loose @13:53:15.74 Elbow.configure :loose @13:53:15.85 SC.configure :loose @13:53:15.94 SE.configure :default @13:53:15.98 CH.coast @13:53:16.03 CH.configure :default @13:53:16.06 PH.coast @13:53:16.11 PH.configure :default @13:53:16.14 All dwarf controllers running firmware version 3.85 @13:53:16.25 Valve.reset @13:53:16.48 Intake.select :close @13:53:16.52 Exhaust.select :close @13:53:16.55 CTV.dial :air @13:53:16.63 PSV.dial :air @13:53:16.70 CSV.dial :waste1 @13:53:16.78
Forearm.reconfigure :loose @13:53:16.82 Forearm.home.seek :retract @13:53:17.17 SSV.dial :spit,avoiding: :exhaust @13:53:17.56 Hand.close @13:53:17.61
Elbow.jog 1500 @13:53:17.74 PS.reconfigure :default @13:53:17.78 PS.home.seek 0ml @13:53:17.99 CS.reconfigure :fast @13:53:18.02 SS.reconfigure :default @13:53:18.13 CS.home.seek 0ml @13:53:18.14 SS.home.seek 0ml @13:53:18.26
Elbow.reconfigure :loose @13:53:18.30 Elbow.home.seek :clear @13:53:22.84 SE.reconfigure :default @13:53:22.88 SE.home.seek :down @13:53:23.47 SC.reconfigure :tight @13:53:23.51 SC.home.seek :initial @13:53:26.01 PS.stop @13:53:51.91
SC.configure :limp @13:53:51.96 Forearm.seek :retract @13:53:52.02 Elbow.seek :hammer @13:53:53.06 Forearm.reconfigure :tight @13:53:53.10 Forearm.seek retracted @13:53:53.35 PC.reconfigure :default @13:53:53.37
CC.reconfigure :default @13:53:53.46 CC.home.seek :open @13:53:53.47 PC.home.seek :open @13:53:57.01
Phase [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] @13:53:57.02 Notice.email "At 13:53:56 began in tube 3: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2/mission/DA_WCR_13023.rb:14\n{1 => 33,\n 2 => 19,\n 3 => 14,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@13:53:56, 17% flow, 16.9C, 34% humidity, 14.7psia, 13.734V, 0.393A, 0.381A avg, 5.40W",Subject: "Started DA_WCR_13023 phase initialPurge; da [1000, 1000] #da and wsr are run simultanelously" @13:53:57.05
Starting initialPurge @13:53:57.07 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @13:53:57.13 Hand.open @13:53:57.88 Elbow.reconfigure :tight @13:53:57.94 Elbow.seek FlushPuck::Garage @13:53:59.85 Elbow.reconfigure :loose @13:53:59.94 Forearm.reconfigure :loose @13:53:59.98 Forearm.seek :garage @13:54:01.05 Hand.close @13:54:01.77 Forearm.seek :retract @13:54:02.79 Elbow.reconfigure :tight @13:54:02.80 upsync @13:54:02.85
Elbow.seek CC @13:54:05.80 Elbow.reconfigure :loose @13:54:05.90 Forearm.reconfigure :tight @13:54:05.94 Forearm.seek CC @13:54:07.25 CC.seek :closed @13:54:11.78 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.35 @13:54:11.84 Hand.open @13:54:12.46 Forearm.reconfigure :loose @13:54:12.50 Forearm.seek :retract @13:54:13.45 Flushing the Collection Valve::Manifold @13:54:13.58 CSV.dial CSR @13:54:14.91 Pulling 5ml of CSR.flush @13:54:14.92 CSR.select :flush @13:54:15.03 CS.reconfigure :default @13:54:15.07 CS.seek 5ml @13:54:28.68 delay 5 seconds @13:54:33.70 CSR.select :air @13:54:33.85 CS.reconfigure :fast @13:54:33.89 CS.seek 7.499ml @13:54:40.68 CSV.dial :waste @13:54:41.95 CS.reconfigure :default @13:54:41.99 CS.seek 0ml @13:55:02.03 Purging external Intake @13:55:02.14 CSV.dial :flush @13:55:02.58 CS.reconfigure :fast @13:55:02.62 CS.seek 10ml @13:55:26.99 delay 5 seconds @13:55:32.01 CSV.dial CBV @13:55:32.04
CBV.dial CSV @13:55:32.79 CTV.dial :intake @13:55:33.59 Intake.select :open @13:55:33.70 CS.seek 0ml @13:55:57.15 Intake.select :close @13:55:57.25 CTV.dial :air @13:55:58.04 Flushing upper manifold @13:55:58.15 CSV.dial :flush @13:55:58.89 CS.seek 5ml @13:56:11.49 delay 5 seconds @13:56:16.51 CSV.dial :air @13:56:17.01 CS.seek 7ml @13:56:22.46 CSV.dial CBV @13:56:22.49
CBV.dial CSV @13:56:23.53 CTV.dial :air @13:56:23.55 CS.seek 0ml @13:56:40.15 delay 7 seconds @13:56:47.18 CS.seek 3ml @13:56:55.18 CSV.dial :waste @13:56:55.63 CS.seek 0ml @13:57:03.41 CSV.dial :flush @13:57:03.84 CS.seek 3ml @13:57:11.84 delay 5 seconds @13:57:16.85 CSV.dial :air @13:57:17.35 CS.seek 5ml @13:57:22.99 CSV.dial :air @13:57:23.01 CS.seek 0ml @13:57:35.18 delay 7 seconds @13:57:42.21 CS.seek 3ml @13:57:50.21 CSV.dial :waste @13:57:50.94 CS.seek 0ml @13:57:58.65 Garaging FlushPuck in Collection Clamp @13:57:58.72 Elbow.reconfigure :tight @13:57:58.76 Elbow.seek CC @13:57:59.14 Elbow.reconfigure :loose @13:57:59.23 Forearm.seek CC @13:58:00.20 Hand.close @13:58:00.77 CC.seek :open @13:58:05.38 Forearm.seek :retract @13:58:06.33 Elbow.reconfigure :tight @13:58:06.37 Elbow.seek :garage @13:58:09.33 Elbow.reconfigure :loose @13:58:09.42 Forearm.reconfigure :tight @13:58:09.46 Forearm.seek :garage @13:58:10.46 Hand.open @13:58:11.51 Forearm.reconfigure :loose @13:58:11.55 Forearm.seek :retract @13:58:12.53 da sampling at most 1000ml, wcr at most 1000ml @13:58:12.57 Loading sh1 puck from tube 3 into Collection Clamp @13:58:12.58 DAfans.email "Started da in tube 3 at 13:58:12UTC30-Jan-23\n{1 => 33,\n 2 => 19,\n 3 => 14,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@13:53:56, 17% flow, 16.9C, 34% humidity, 14.7psia, 13.734V, 0.393A, 0.381A avg, 5.40W",Subject: "da sampling at most 1000ml, wcr at most 1000ml" @13:58:12.85
SC.reconfigure :tight @13:58:12.89 SC.seek 3 @13:58:16.48 SC.configure :limp @13:58:16.70 Elbow.reconfigure :tight @13:58:16.75 Elbow.seek 3 @13:58:17.76 upsync @13:58:18.69
Forearm.reconfigure :tight @13:58:18.74 Forearm.seek 3 + 450 counts @13:58:19.78 SE.reconfigure :pick @13:58:19.83 SE.seek :beamBreak @13:58:22.15 SE.configure :default @13:58:22.29 Elbow.seek 3 @13:58:22.46 Forearm.seek 3 + 300 counts @13:58:22.65 Forearm.reconfigure :loose @13:58:22.70 Hand.close @13:58:23.28 SE.seek :down @13:58:23.47
Forearm.seek :retract @13:58:24.47 Elbow.seek CC @13:58:25.16 SE.stop @13:58:26.08
Elbow.reconfigure :loose @13:58:26.17 Forearm.reconfigure :tight @13:58:26.22 Forearm.seek CC @13:58:27.48 CC.seek :closed @13:58:31.99 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.23 @13:58:32.00 Hand.open @13:58:32.69 Forearm.reconfigure :loose @13:58:32.74 Forearm.seek :retract @13:58:33.73 collectSample 1000ml w/bubble point of 24psi @13:58:33.75 Priming 7ml Intake.select :close @13:58:33.76 Exhaust.select :close @13:58:33.78 SS.hold @13:58:33.97 Calibrating pressure and temperature sensors @13:58:34.00 CTV.dial :air @13:58:34.01 SSV.dial :spit,avoiding: :exhaust @13:58:34.03 SS.reconfigure :fast @13:58:34.07 SS.seek 5ml @13:58:37.06 SSV.dial CBV @13:58:37.07
CBV.dial SSV @13:58:38.41 delay 3.8 seconds @13:58:42.33 SSV.dial :spit @13:58:43.65 SS.seek 0ml @13:58:46.22 SSV.dial CBV @13:58:47.50 CTV.dial :intake @13:58:48.27 SSV.dial CBV @13:58:48.30
CBV.dial SSV @13:58:48.31 Allowing 40s for 7.0ml of remaining 7.0ml @13:58:48.32 Intake.select :open @13:58:50.47 Intake @15.4psia, vacuum from 9.9 to 12.9psi @13:58:50.49 SS.reconfigure :inhaling 2.20ml/s @13:58:50.53 SS.seek 7ml @13:58:57.89 Stroke completed -- waiting for pressure to equalize ... @13:58:57.90 SS.reconfigure :exhale @13:58:57.94 SS.hold @13:58:59.13 Intake.select :close @13:58:59.27 SSV.dial :exhaust @13:59:00.02 Exhaust.select :open @13:59:00.13 SS.seek 0ml @13:59:03.86 Exhaust.select :close @13:59:03.97 Sampling 1000.0ml Intake.select :close @13:59:03.98 Exhaust.select :close @13:59:03.99 SS.reconfigure :default @13:59:04.03 SS.hold @13:59:04.21 CTV.dial :intake @13:59:04.23 SSV.dial CBV @13:59:04.26
CBV.dial SSV @13:59:05.02 Allowing 130s for 25.0ml of remaining 1000.0ml @13:59:05.03 Intake.select :open @13:59:07.18 Intake @15.4psia, vacuum from 9.9 to 12.9psi @13:59:07.20 SS.reconfigure :inhaling 2.20ml/s @13:59:07.24 SS.seek 25ml @13:59:18.99 Vacuum too high -- waiting up to 40 seconds @13:59:19.00 SS.reconfigure :exhale @13:59:19.04 SS.hold @13:59:19.73 Intake @11.1psia, vacuum from 5.6 to 8.6psi @13:59:19.75 SS.reconfigure :inhaling 1.98ml/s @13:59:19.79 SS.seek 25ml @13:59:23.89 Stroke completed -- waiting for pressure to equalize ... @13:59:23.90 SS.reconfigure :exhale @13:59:23.94 SS.hold @13:59:29.87 Intake.select :close @13:59:30.01 SSV.dial :exhaust @13:59:30.77 Exhaust.select :open @13:59:30.87 SS.seek 0ml @13:59:39.85 Exhaust.select :close @13:59:39.95 SSV.dial CBV @13:59:39.97
CBV.dial SSV @13:59:40.74 Allowing 130s for 25.0ml of remaining 975.0ml @13:59:40.75 Intake.select :open @13:59:42.90 Intake @15.0psia, vacuum from 9.5 to 12.5psi @13:59:42.92 SS.reconfigure :inhaling 1.98ml/s @13:59:42.96 SS.seek 25ml @13:59:56.31 Vacuum too high -- waiting up to 40 seconds @13:59:56.32 SS.reconfigure :exhale @13:59:56.36 SS.hold @13:59:57.00 Intake @11.3psia, vacuum from 5.8 to 8.8psi @13:59:57.02 SS.reconfigure :inhaling 1.79ml/s @13:59:57.06 SS.seek 25ml @14:00:00.45 Stroke completed -- waiting for pressure to equalize ... @14:00:00.46 SS.reconfigure :exhale @14:00:00.50 SS.hold @14:00:07.95 Intake.select :close @14:00:08.09 SSV.dial :exhaust @14:00:08.85 Exhaust.select :open @14:00:08.95 SS.seek 0ml @14:00:17.75 Exhaust.select :close @14:00:17.86 SSV.dial CBV @14:00:17.88
CBV.dial SSV @14:00:18.64 Allowing 130s for 25.0ml of remaining 950.0ml @14:00:18.65 Intake.select :open @14:00:20.81 Intake @15.1psia, vacuum from 9.6 to 12.6psi @14:00:20.82 SS.reconfigure :inhaling 1.79ml/s @14:00:20.86 SS.seek 25ml @14:00:36.90 Vacuum too high -- waiting up to 40 seconds @14:00:36.91 SS.reconfigure :exhale @14:00:36.95 SS.hold @14:00:37.54 Intake @11.5psia, vacuum from 6.0 to 9.0psi @14:00:37.56 SS.reconfigure :inhaling 1.61ml/s @14:00:37.60 SS.seek 25ml @14:00:39.47 Stroke completed -- waiting for pressure to equalize ... @14:00:39.48 SS.reconfigure :exhale @14:00:39.52 SS.hold @14:00:46.97 Intake.select :close @14:00:47.11 SSV.dial :exhaust @14:00:47.90 Exhaust.select :open @14:00:48.00 SS.seek 0ml @14:00:57.01 Exhaust.select :close @14:00:57.12 SSV.dial CBV @14:00:57.14
CBV.dial SSV @14:00:57.91 Allowing 130s for 25.0ml of remaining 925.0ml @14:00:57.92 Intake.select :open @14:01:00.07 Intake @15.0psia, vacuum from 9.5 to 12.5psi @14:01:00.09 SS.reconfigure :inhaling 1.61ml/s @14:01:00.13 SS.seek 25ml @14:01:18.50 Stroke completed -- waiting for pressure to equalize ... @14:01:18.51 SS.reconfigure :exhale @14:01:18.55 SS.hold @14:01:27.14 Intake.select :close @14:01:27.28 SSV.dial :exhaust @14:01:28.03 Exhaust.select :open @14:01:28.14 SS.seek 0ml @14:01:36.89 Exhaust.select :close @14:01:36.99 SSV.dial CBV @14:01:37.01
CBV.dial SSV @14:01:37.78 Allowing 130s for 25.0ml of remaining 900.0ml @14:01:37.79 Intake.select :open @14:01:39.94 Intake @15.0psia, vacuum from 9.5 to 12.5psi @14:01:39.96 SS.reconfigure :inhaling 1.61ml/s @14:01:40.00 SS.seek 25ml @14:01:58.51 Stroke completed -- waiting for pressure to equalize ... @14:01:58.52 SS.reconfigure :exhale @14:01:58.57 SS.hold @14:02:07.10 Intake.select :close @14:02:07.23 SSV.dial :exhaust @14:02:08.00 Exhaust.select :open @14:02:08.11 SS.seek 0ml @14:02:16.89 Exhaust.select :close @14:02:16.99 SSV.dial CBV @14:02:17.01
CBV.dial SSV @14:02:17.78 Allowing 130s for 25.0ml of remaining 875.0ml @14:02:17.79 Intake.select :open @14:02:19.95 Intake @15.0psia, vacuum from 9.5 to 12.5psi @14:02:19.96 SS.reconfigure :inhaling 1.61ml/s @14:02:20.00 SS.seek 25ml @14:02:37.46 Vacuum too high -- waiting up to 40 seconds @14:02:37.47 SS.reconfigure :exhale @14:02:37.51 SS.hold @14:02:38.06 Intake @12.0psia, vacuum from 6.5 to 9.5psi @14:02:38.07 SS.reconfigure :inhaling 1.45ml/s @14:02:38.12 SS.seek 25ml @14:02:39.95 Stroke completed -- waiting for pressure to equalize ... @14:02:39.96 SS.reconfigure :exhale @14:02:40.00 SS.hold @14:02:48.54 Intake.select :close @14:02:48.68 SSV.dial :exhaust @14:02:49.44 Exhaust.select :open @14:02:49.54 SS.seek 0ml @14:02:58.57 Exhaust.select :close @14:02:58.68 SSV.dial CBV @14:02:58.70
CBV.dial SSV @14:02:59.45 Allowing 130s for 25.0ml of remaining 850.0ml @14:02:59.46 Intake.select :open @14:03:01.62 Intake @15.1psia, vacuum from 9.6 to 12.6psi @14:03:01.64 SS.reconfigure :inhaling 1.45ml/s @14:03:01.68 SS.seek 25ml @14:03:21.05 Vacuum too high -- waiting up to 40 seconds @14:03:21.06 SS.reconfigure :exhale @14:03:21.10 SS.hold @14:03:22.73 Intake @12.3psia, vacuum from 6.8 to 9.8psi @14:03:22.75 SS.reconfigure :inhaling 1.30ml/s @14:03:22.79 SS.seek 25ml @14:03:23.09 Stroke completed -- waiting for pressure to equalize ... @14:03:23.10 SS.reconfigure :exhale @14:03:23.14 SS.hold @14:03:30.58 Intake.select :close @14:03:30.72 SSV.dial :exhaust @14:03:31.47 Exhaust.select :open @14:03:31.57 SS.seek 0ml @14:03:40.62 Exhaust.select :close @14:03:40.72 SSV.dial CBV @14:03:40.75
CBV.dial SSV @14:03:41.50 Allowing 130s for 25.0ml of remaining 825.0ml @14:03:41.51 Intake.select :open @14:03:43.66 Intake @15.1psia, vacuum from 9.6 to 12.6psi @14:03:43.68 SS.reconfigure :inhaling 1.30ml/s @14:03:43.72 SS.seek 25ml @14:04:05.50 Stroke completed -- waiting for pressure to equalize ... @14:04:05.51 SS.reconfigure :exhale @14:04:05.55 SS.hold @14:04:14.07 Intake.select :close @14:04:14.21 SSV.dial :exhaust @14:04:14.95 Exhaust.select :open @14:04:15.06 SS.seek 0ml @14:04:24.09 Exhaust.select :close @14:04:24.19 SSV.dial CBV @14:04:24.21
CBV.dial SSV @14:04:24.97 Allowing 130s for 25.0ml of remaining 800.0ml @14:04:24.98 Intake.select :open @14:04:27.13 Intake @15.1psia, vacuum from 9.6 to 12.6psi @14:04:27.14 SS.reconfigure :inhaling 1.30ml/s @14:04:27.19 SS.seek 25ml @14:04:48.74 Stroke completed -- waiting for pressure to equalize ... @14:04:48.75 SS.reconfigure :exhale @14:04:48.79 SS.hold @14:04:58.45 Intake.select :close @14:04:58.59 SSV.dial :exhaust @14:04:59.33 Exhaust.select :open @14:04:59.92 SS.seek 0ml @14:05:08.90 Exhaust.select :close @14:05:09.00 SSV.dial CBV @14:05:09.03
CBV.dial SSV @14:05:09.79 Allowing 130s for 25.0ml of remaining 775.0ml @14:05:09.80 Intake.select :open @14:05:11.96 Intake @15.2psia, vacuum from 9.7 to 12.7psi @14:05:11.98 SS.reconfigure :inhaling 1.30ml/s @14:05:12.02 SS.seek 25ml @14:05:33.21 Vacuum too high -- waiting up to 40 seconds @14:05:33.22 SS.reconfigure :exhale @14:05:33.26 SS.hold @14:05:36.28 Intake @12.9psia, vacuum from 7.4 to 10.4psi @14:05:36.29 SS.reconfigure :inhaling 1.17ml/s @14:05:36.34 SS.seek 25ml @14:05:37.21 Stroke completed -- waiting for pressure to equalize ... @14:05:37.22 SS.reconfigure :exhale @14:05:37.26 SS.hold @14:05:43.76 Intake.select :close @14:05:43.90 SSV.dial :exhaust @14:05:44.67 Exhaust.select :open @14:05:44.77 SS.seek 0ml @14:05:53.56 Exhaust.select :close @14:05:53.66 SSV.dial CBV @14:05:53.68
CBV.dial SSV @14:05:54.45 Allowing 130s for 25.0ml of remaining 750.0ml @14:05:54.46 Intake.select :open @14:05:56.61 Intake @15.3psia, vacuum from 9.8 to 12.8psi @14:05:56.63 SS.reconfigure :inhaling 1.17ml/s @14:05:56.67 SS.seek 25ml @14:06:20.79 Stroke completed -- waiting for pressure to equalize ... @14:06:20.80 SS.reconfigure :exhale @14:06:20.84 SS.hold @14:06:30.49 Intake.select :close @14:06:30.63 SSV.dial :exhaust @14:06:31.39 Exhaust.select :open @14:06:31.50 SS.seek 0ml @14:06:40.34 Exhaust.select :close @14:06:40.44 SSV.dial CBV @14:06:40.46
CBV.dial SSV @14:06:41.23 Allowing 130s for 25.0ml of remaining 725.0ml @14:06:41.24 Intake.select :open @14:06:43.39 Intake @15.2psia, vacuum from 9.7 to 12.7psi @14:06:43.41 SS.reconfigure :inhaling 1.17ml/s @14:06:43.45 SS.seek 25ml @14:07:06.42 Vacuum too high -- waiting up to 40 seconds @14:07:06.43 SS.reconfigure :exhale @14:07:06.47 SS.hold @14:07:10.62 Intake @13.2psia, vacuum from 7.7 to 10.7psi @14:07:10.63 SS.reconfigure :inhaling 1.06ml/s @14:07:10.67 SS.seek 25ml @14:07:12.33 Stroke completed -- waiting for pressure to equalize ... @14:07:12.34 SS.reconfigure :exhale @14:07:12.38 SS.hold @14:07:17.90 Intake.select :close @14:07:18.04 SSV.dial :exhaust @14:07:18.81 Exhaust.select :open @14:07:18.91 SS.seek 0ml @14:07:27.73 Exhaust.select :close @14:07:27.83 SSV.dial CBV @14:07:27.85
CBV.dial SSV @14:07:28.62 Allowing 130s for 25.0ml of remaining 700.0ml @14:07:28.63 Intake.select :open @14:07:30.78 Intake @15.2psia, vacuum from 9.7 to 12.7psi @14:07:30.80 SS.reconfigure :inhaling 1.06ml/s @14:07:30.84 SS.seek 25ml @14:07:57.35 Stroke completed -- waiting for pressure to equalize ... @14:07:57.36 SS.reconfigure :exhale @14:07:57.40 SS.hold @14:08:07.65 Intake.select :close @14:08:07.79 SSV.dial :exhaust @14:08:08.55 Exhaust.select :open @14:08:08.66 SS.seek 0ml @14:08:17.43 Exhaust.select :close @14:08:17.53 SSV.dial CBV @14:08:17.55
CBV.dial SSV @14:08:18.32 Allowing 130s for 25.0ml of remaining 675.0ml @14:08:18.33 Intake.select :open @14:08:20.49 Intake @15.0psia, vacuum from 9.5 to 12.5psi @14:08:20.50 SS.reconfigure :inhaling 1.06ml/s @14:08:20.54 SS.seek 25ml @14:08:47.29 Stroke completed -- waiting for pressure to equalize ... @14:08:47.30 SS.reconfigure :exhale @14:08:47.34 SS.hold @14:08:58.45 Intake.select :close @14:08:58.58 SSV.dial :exhaust @14:08:59.33 Exhaust.select :open @14:08:59.44 SS.seek 0ml @14:09:08.19 Exhaust.select :close @14:09:08.30 SSV.dial CBV @14:09:08.35
CBV.dial SSV @14:09:09.07 Allowing 130s for 25.0ml of remaining 650.0ml @14:09:09.08 Intake.select :open @14:09:11.23 Intake @15.2psia, vacuum from 9.7 to 12.7psi @14:09:11.25 SS.reconfigure :inhaling 1.06ml/s @14:09:11.29 SS.seek 25ml @14:09:31.80 Vacuum too high -- waiting up to 40 seconds @14:09:31.81 SS.reconfigure :exhale @14:09:31.85 SS.hold @14:09:36.00 Intake @13.4psia, vacuum from 7.9 to 10.9psi @14:09:36.02 SS.reconfigure :inhaling 0.95ml/s @14:09:36.06 SS.seek 25ml @14:09:43.14 Stroke completed -- waiting for pressure to equalize ... @14:09:43.15 SS.reconfigure :exhale @14:09:43.19 SS.hold @14:09:51.64 Intake.select :close @14:09:51.77 SSV.dial :exhaust @14:09:53.38 Exhaust.select :open @14:09:53.49 SS.seek 0ml @14:10:02.28 Exhaust.select :close @14:10:02.39 SSV.dial CBV @14:10:02.43
CBV.dial SSV @14:10:03.16 Allowing 130s for 25.0ml of remaining 625.0ml @14:10:03.17 Intake.select :open @14:10:05.33 Intake @15.1psia, vacuum from 9.6 to 12.6psi @14:10:05.34 SS.reconfigure :inhaling 0.95ml/s @14:10:05.38 SS.seek 25ml @14:10:33.64 Vacuum too high -- waiting up to 40 seconds @14:10:33.65 SS.reconfigure :exhale @14:10:33.69 SS.hold @14:10:39.31 Intake @13.7psia, vacuum from 8.2 to 11.2psi @14:10:39.33 SS.reconfigure :inhaling 0.87ml/s @14:10:39.37 SS.seek 25ml @14:10:40.74 Stroke completed -- waiting for pressure to equalize ... @14:10:40.75 SS.reconfigure :exhale @14:10:40.79 SS.hold @14:10:47.18 Intake.select :close @14:10:47.31 SSV.dial :exhaust @14:10:48.07 Exhaust.select :open @14:10:48.17 SS.seek 0ml @14:10:57.17 Exhaust.select :close @14:10:57.77 SSV.dial CBV @14:10:57.78
CBV.dial SSV @14:10:58.54 Allowing 130s for 25.0ml of remaining 600.0ml @14:10:58.55 Intake.select :open @14:11:00.70 Intake @15.1psia, vacuum from 9.6 to 12.6psi @14:11:00.72 SS.reconfigure :inhaling 0.87ml/s @14:11:00.76 SS.seek 25ml @14:11:31.72 Stroke completed -- waiting for pressure to equalize ... @14:11:31.73 SS.reconfigure :exhale @14:11:31.77 SS.hold @14:11:44.40 Intake.select :close @14:11:44.54 SSV.dial :exhaust @14:11:45.28 Exhaust.select :open @14:11:45.39 SS.seek 0ml @14:11:54.53 Exhaust.select :close @14:11:54.64 SSV.dial CBV @14:11:54.66
CBV.dial SSV @14:11:55.43 Allowing 130s for 25.0ml of remaining 575.0ml @14:11:55.44 Intake.select :open @14:11:57.59 Intake @15.1psia, vacuum from 9.6 to 12.6psi @14:11:57.61 SS.reconfigure :inhaling 0.87ml/s @14:11:57.65 SS.seek 25ml @14:12:27.38 Vacuum too high -- waiting up to 40 seconds @14:12:27.39 SS.reconfigure :exhale @14:12:27.44 SS.hold @14:12:33.92 Intake @13.8psia, vacuum from 8.3 to 11.3psi @14:12:33.93 SS.reconfigure :inhaling 0.78ml/s @14:12:33.98 SS.seek 25ml @14:12:36.02 Stroke completed -- waiting for pressure to equalize ... @14:12:36.03 SS.reconfigure :exhale @14:12:36.07 SS.hold @14:12:42.54 Intake.select :close @14:12:42.68 SSV.dial :exhaust @14:12:43.44 Exhaust.select :open @14:12:43.55 SS.seek 0ml @14:12:52.53 Exhaust.select :close @14:12:52.64 SSV.dial CBV @14:12:52.66
CBV.dial SSV @14:12:53.43 Allowing 130s for 25.0ml of remaining 550.0ml @14:12:53.44 Intake.select :open @14:12:55.59 Intake @15.1psia, vacuum from 9.6 to 12.6psi @14:12:55.61 SS.reconfigure :inhaling 0.78ml/s @14:12:55.65 SS.seek 25ml @14:13:29.94 Stroke completed -- waiting for pressure to equalize ... @14:13:29.95 SS.reconfigure :exhale @14:13:29.99 SS.hold @14:13:44.24 Intake.select :close @14:13:44.38 SSV.dial :exhaust @14:13:45.14 Exhaust.select :open @14:13:45.25 SS.seek 0ml @14:13:54.03 Exhaust.select :close @14:13:54.13 SSV.dial CBV @14:13:54.16
CBV.dial SSV @14:13:54.92 Allowing 130s for 25.0ml of remaining 525.0ml @14:13:54.93 Intake.select :open @14:13:57.09 Intake @15.0psia, vacuum from 9.5 to 12.5psi @14:13:57.48 SS.reconfigure :inhaling 0.78ml/s @14:13:57.61 SS.seek 25ml @14:14:27.41 Vacuum too high -- waiting up to 40 seconds @14:14:27.42 SS.reconfigure :exhale @14:14:27.47 SS.hold @14:14:33.96 Intake @14.1psia, vacuum from 8.6 to 11.6psi @14:14:33.97 SS.reconfigure :inhaling 0.70ml/s @14:14:34.02 SS.seek 25ml @14:14:39.54 Stroke completed -- waiting for pressure to equalize ... @14:14:39.55 SS.reconfigure :exhale @14:14:39.60 SS.hold @14:14:48.05 Intake.select :close @14:14:48.18 SSV.dial :exhaust @14:14:48.94 Exhaust.select :open @14:14:49.04 SS.seek 0ml @14:14:57.81 Exhaust.select :close @14:14:57.91 SSV.dial CBV @14:14:57.94
CBV.dial SSV @14:14:58.70 Allowing 130s for 25.0ml of remaining 500.0ml @14:14:58.71 Intake.select :open @14:15:00.87 Intake @15.1psia, vacuum from 9.6 to 12.6psi @14:15:00.88 SS.reconfigure :inhaling 0.70ml/s @14:15:00.93 SS.seek 25ml @14:15:39.09 Stroke completed -- waiting for pressure to equalize ... @14:15:39.10 SS.reconfigure :exhale @14:15:39.14 SS.hold @14:15:55.30 Intake.select :close @14:15:55.44 SSV.dial :exhaust @14:15:56.20 Exhaust.select :open @14:15:56.31 SS.seek 0ml @14:16:05.31 Exhaust.select :close @14:16:05.42 SSV.dial CBV @14:16:05.43
CBV.dial SSV @14:16:06.20 Allowing 130s for 25.0ml of remaining 475.0ml @14:16:06.21 Intake.select :open @14:16:08.36 Intake @15.2psia, vacuum from 9.7 to 12.7psi @14:16:08.38 SS.reconfigure :inhaling 0.70ml/s @14:16:08.42 SS.seek 25ml @14:16:40.75 Vacuum too high -- waiting up to 40 seconds @14:16:40.76 SS.reconfigure :exhale @14:16:40.80 SS.hold @14:16:48.25 Intake @14.3psia, vacuum from 8.8 to 11.8psi @14:16:48.27 SS.reconfigure :inhaling 0.63ml/s @14:16:48.31 SS.seek 25ml @14:16:54.53 Stroke completed -- waiting for pressure to equalize ... @14:16:54.54 SS.reconfigure :exhale @14:16:54.58 SS.hold @14:17:05.79 Intake.select :close @14:17:05.92 SSV.dial :exhaust @14:17:06.68 Exhaust.select :open @14:17:06.79 SS.seek 0ml @14:17:15.79 Exhaust.select :close @14:17:15.90 SSV.dial CBV @14:17:15.93
CBV.dial SSV @14:17:16.67 Allowing 130s for 25.0ml of remaining 450.0ml @14:17:16.68 Intake.select :open @14:17:18.84 Intake @15.2psia, vacuum from 9.7 to 12.7psi @14:17:18.86 SS.reconfigure :inhaling 0.63ml/s @14:17:18.90 SS.seek 25ml @14:17:56.10 Vacuum too high -- waiting up to 40 seconds @14:17:56.11 SS.reconfigure :exhale @14:17:56.15 SS.hold @14:18:03.60 Intake @14.2psia, vacuum from 8.7 to 11.7psi @14:18:03.62 SS.reconfigure :inhaling 0.57ml/s @14:18:03.66 SS.seek 25ml @14:18:08.90 Stroke completed -- waiting for pressure to equalize ... @14:18:08.91 SS.reconfigure :exhale @14:18:08.95 SS.hold @14:18:20.07 Intake.select :close @14:18:20.21 SSV.dial :exhaust @14:18:20.95 Exhaust.select :open @14:18:21.06 SS.seek 0ml @14:18:29.82 Exhaust.select :close @14:18:29.92 SSV.dial CBV @14:18:29.98
CBV.dial SSV @14:18:30.68 Allowing 130s for 25.0ml of remaining 425.0ml @14:18:30.69 Intake.select :open @14:18:32.85 Intake @15.1psia, vacuum from 9.6 to 12.6psi @14:18:32.87 SS.reconfigure :inhaling 0.57ml/s @14:18:32.91 SS.seek 25ml @14:19:19.29 Stroke completed -- waiting for pressure to equalize ... @14:19:19.30 SS.reconfigure :exhale @14:19:19.34 SS.hold @14:19:37.63 Intake.select :close @14:19:37.77 SSV.dial :exhaust @14:19:38.51 Exhaust.select :open @14:19:38.62 SS.seek 0ml @14:19:47.41 Exhaust.select :close @14:19:47.52 SSV.dial CBV @14:19:47.54
CBV.dial SSV @14:19:48.29 Allowing 130s for 25.0ml of remaining 400.0ml @14:19:48.30 Intake.select :open @14:19:50.45 Intake @15.2psia, vacuum from 9.7 to 12.7psi @14:19:50.47 SS.reconfigure :inhaling 0.57ml/s @14:19:50.51 SS.seek 25ml @14:20:29.38 Vacuum too high -- waiting up to 40 seconds @14:20:29.39 SS.reconfigure :exhale @14:20:29.43 SS.hold @14:20:37.89 Intake @14.2psia, vacuum from 8.7 to 11.7psi @14:20:37.90 SS.reconfigure :inhaling 0.51ml/s @14:20:37.95 SS.seek 25ml @14:20:46.44 Stroke completed -- waiting for pressure to equalize ... @14:20:46.45 SS.reconfigure :exhale @14:20:46.50 SS.hold @14:20:59.13 Intake.select :close @14:20:59.26 SSV.dial :exhaust @14:21:00.01 Exhaust.select :open @14:21:00.12 SS.seek 0ml @14:21:09.21 Exhaust.select :close @14:21:09.32 SSV.dial CBV @14:21:09.33
CBV.dial SSV @14:21:10.09 Allowing 130s for 25.0ml of remaining 375.0ml @14:21:10.10 Intake.select :open @14:21:12.25 Intake @15.1psia, vacuum from 9.6 to 12.6psi @14:21:12.27 SS.reconfigure :inhaling 0.51ml/s @14:21:12.31 SS.seek 25ml @14:22:02.97 Stroke completed -- waiting for pressure to equalize ... @14:22:02.98 SS.reconfigure :exhale @14:22:03.02 SS.hold @14:22:23.33 Intake.select :close @14:22:23.47 SSV.dial :exhaust @14:22:24.21 Exhaust.select :open @14:22:24.32 SS.seek 0ml @14:22:33.18 Exhaust.select :close @14:22:33.28 SSV.dial CBV @14:22:33.30
CBV.dial SSV @14:22:34.05 Allowing 130s for 25.0ml of remaining 350.0ml @14:22:34.06 Intake.select :open @14:22:36.22 Intake @15.1psia, vacuum from 9.6 to 12.6psi @14:22:36.24 SS.reconfigure :inhaling 0.51ml/s @14:22:36.28 SS.seek 25ml @14:23:17.22 Vacuum too high -- waiting up to 40 seconds @14:23:17.23 SS.reconfigure :exhale @14:23:17.27 SS.hold @14:23:26.90 Intake @14.3psia, vacuum from 8.8 to 11.8psi @14:23:26.92 SS.reconfigure :inhaling 0.47ml/s @14:23:26.96 SS.seek 25ml @14:23:38.94 Stroke completed -- waiting for pressure to equalize ... @14:23:38.95 SS.reconfigure :exhale @14:23:38.99 SS.hold @14:23:53.30 Intake.select :close @14:23:53.43 SSV.dial :exhaust @14:23:54.18 Exhaust.select :open @14:23:54.29 SS.seek 0ml @14:24:03.35 Exhaust.select :close @14:24:03.45 SSV.dial CBV @14:24:03.47
CBV.dial SSV @14:24:04.23 Allowing 130s for 25.0ml of remaining 325.0ml @14:24:04.24 Intake.select :open @14:24:06.39 Intake @15.1psia, vacuum from 9.6 to 12.6psi @14:24:06.41 SS.reconfigure :inhaling 0.47ml/s @14:24:06.45 SS.seek 25ml @14:25:01.56 Vacuum too high -- waiting up to 40 seconds @14:25:01.57 SS.reconfigure :exhale @14:25:01.61 SS.hold @14:25:14.15 Intake @14.5psia, vacuum from 9.0 to 12.0psi @14:25:14.17 SS.reconfigure :inhaling 0.43ml/s @14:25:14.21 SS.seek 25ml @14:25:15.41 Stroke completed -- waiting for pressure to equalize ... @14:25:15.42 SS.reconfigure :exhale @14:25:15.46 SS.hold @14:25:26.64 Intake.select :close @14:25:26.77 SSV.dial :exhaust @14:25:27.52 Exhaust.select :open @14:25:27.63 SS.seek 0ml @14:25:36.42 Exhaust.select :close @14:25:36.53 SSV.dial CBV @14:25:36.55
CBV.dial SSV @14:25:37.30 Allowing 130s for 25.0ml of remaining 300.0ml @14:25:37.31 Intake.select :open @14:25:39.46 Intake @15.1psia, vacuum from 9.6 to 12.6psi @14:25:39.48 SS.reconfigure :inhaling 0.43ml/s @14:25:39.52 SS.seek 25ml @14:26:43.30 Stroke completed -- waiting for pressure to equalize ... @14:26:43.31 SS.reconfigure :exhale @14:26:43.35 SS.hold @14:27:05.69 Intake.select :close @14:27:05.83 SSV.dial :exhaust @14:27:06.56 Exhaust.select :open @14:27:06.67 SS.seek 0ml @14:27:15.45 Exhaust.select :close @14:27:15.56 SSV.dial CBV @14:27:15.61
CBV.dial SSV @14:27:16.31 Allowing 130s for 25.0ml of remaining 275.0ml @14:27:16.32 Intake.select :open @14:27:18.48 Intake @15.1psia, vacuum from 9.6 to 12.6psi @14:27:18.49 SS.reconfigure :inhaling 0.43ml/s @14:27:18.54 SS.seek 25ml @14:28:13.52 Vacuum too high -- waiting up to 40 seconds @14:28:13.53 SS.reconfigure :exhale @14:28:13.58 SS.hold @14:28:26.57 Intake @14.7psia, vacuum from 9.2 to 12.2psi @14:28:26.58 SS.reconfigure :inhaling 0.38ml/s @14:28:26.63 SS.seek 25ml @14:28:36.67 Stroke completed -- waiting for pressure to equalize ... @14:28:36.68 SS.reconfigure :exhale @14:28:36.72 SS.hold @14:28:52.99 Intake.select :close @14:28:53.13 SSV.dial :exhaust @14:28:54.34 Exhaust.select :open @14:28:54.45 SS.seek 0ml @14:29:03.44 Exhaust.select :close @14:29:03.54 SSV.dial CBV @14:29:03.56
CBV.dial SSV @14:29:04.33 Allowing 130s for 25.0ml of remaining 250.0ml @14:29:04.34 Intake.select :open @14:29:06.49 Intake @15.2psia, vacuum from 9.7 to 12.7psi @14:29:06.51 SS.reconfigure :inhaling 0.38ml/s @14:29:06.55 SS.seek 25ml @14:30:20.62 Stroke completed -- waiting for pressure to equalize ... @14:30:20.63 SS.reconfigure :exhale @14:30:20.67 SS.hold @14:30:45.02 Intake.select :close @14:30:45.16 SSV.dial :exhaust @14:30:45.90 Exhaust.select :open @14:30:46.01 SS.seek 0ml @14:30:55.07 Exhaust.select :close @14:30:55.18 SSV.dial CBV @14:30:55.20
CBV.dial SSV @14:30:55.96 Allowing 130s for 25.0ml of remaining 225.0ml @14:30:55.97 Intake.select :open @14:30:58.12 Intake @15.1psia, vacuum from 9.6 to 12.6psi @14:30:58.14 SS.reconfigure :inhaling 0.38ml/s @14:30:58.18 SS.seek 25ml @14:32:11.87 Stroke completed -- waiting for pressure to equalize ... @14:32:11.88 SS.reconfigure :exhale @14:32:11.92 SS.hold @14:32:40.35 Intake.select :close @14:32:40.48 SSV.dial :exhaust @14:32:41.23 Exhaust.select :open @14:32:41.81 SS.seek 0ml @14:32:50.67 Exhaust.select :close @14:32:50.77 SSV.dial CBV @14:32:50.79
CBV.dial SSV @14:32:51.54 Allowing 130s for 25.0ml of remaining 200.0ml @14:32:51.55 Intake.select :open @14:32:53.71 Intake @15.0psia, vacuum from 9.5 to 12.5psi @14:32:53.72 SS.reconfigure :inhaling 0.38ml/s @14:32:53.77 SS.seek 25ml @14:33:54.98 Vacuum too high -- waiting up to 40 seconds @14:33:54.99 SS.reconfigure :exhale @14:33:55.04 SS.hold @14:34:09.38 Intake @14.6psia, vacuum from 9.1 to 12.1psi @14:34:09.40 SS.reconfigure :inhaling 0.34ml/s @14:34:09.44 SS.seek 25ml @14:34:25.58 Stroke completed -- waiting for pressure to equalize ... @14:34:25.59 SS.reconfigure :exhale @14:34:25.64 SS.hold @14:34:47.93 Intake.select :close @14:34:48.08 SSV.dial :exhaust @14:34:48.82 Exhaust.select :open @14:34:48.93 SS.seek 0ml @14:34:57.96 Exhaust.select :close @14:34:58.07 SSV.dial CBV @14:34:58.08
CBV.dial SSV @14:34:58.84 Allowing 130s for 25.0ml of remaining 175.0ml @14:34:58.85 Intake.select :open @14:35:01.00 Intake @15.0psia, vacuum from 9.5 to 12.5psi @14:35:01.02 SS.reconfigure :inhaling 0.34ml/s @14:35:01.06 SS.seek 25ml @14:36:28.96 Stroke completed -- waiting for pressure to equalize ... @14:36:28.97 SS.reconfigure :exhale @14:36:29.01 SS.hold @14:37:01.52 Intake.select :close @14:37:01.66 SSV.dial :exhaust @14:37:02.42 Exhaust.select :open @14:37:02.53 SS.seek 0ml @14:37:11.30 Exhaust.select :close @14:37:11.40 SSV.dial CBV @14:37:11.46
CBV.dial SSV @14:37:12.18 Allowing 130s for 25.0ml of remaining 150.0ml @14:37:12.19 Intake.select :open @14:37:14.34 Intake @15.0psia, vacuum from 9.5 to 12.5psi @14:37:14.36 SS.reconfigure :inhaling 0.34ml/s @14:37:14.40 SS.seek 25ml @14:38:40.79 Vacuum too high -- waiting up to 40 seconds @14:38:40.80 SS.reconfigure :exhale @14:38:40.84 SS.hold @14:38:59.39 Intake @14.8psia, vacuum from 9.3 to 12.3psi @14:38:59.41 SS.reconfigure :inhaling 0.31ml/s @14:38:59.45 SS.seek 25ml @14:39:04.13 Stroke completed -- waiting for pressure to equalize ... @14:39:04.14 SS.reconfigure :exhale @14:39:04.18 SS.hold @14:39:22.20 SS.reconfigure :default @14:39:22.24 SS.hold @14:39:22.35 Intake.select :close @14:39:22.48 ShallowSampler::Clogged in MAIN -- Filtering too Slowly after 875.0ml @14:39:22.50 SSV.dial :exhaust @14:39:23.25 Exhaust.select :open @14:39:23.36 SS.reconfigure :exhale @14:39:23.40 SS.seek 0ml @14:39:32.28 Exhaust.select :close @14:39:32.39 Sampled 875.0ml @14:39:32.40 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @14:39:32.41 Intake.select :close @14:39:32.46 CSV.dial :waste @14:39:32.51 CS.seek 0ml @14:39:32.61 SSV.dial CBV,avoiding: :exhaust @14:39:32.64
CBV.dial SSV @14:39:32.69 CSV.dial :air @14:39:33.43 CS.seek 10ml @14:39:34.47
CTV.dial :air @14:39:35.27 delay 15 seconds @14:39:50.29 SSV.dial :spit,avoiding: :exhaust @14:39:51.60 CTV.dial CSV,avoiding: :intake @14:39:51.64
SS.seek 0ml @14:39:51.75 SSV.dial CBV,avoiding: :exhaust @14:39:57.79 CBV.dial between SSV and waste2 @14:39:58.17 CTV.dial CSV,avoiding: :air @14:39:58.20
CSV.dial CTV @14:39:58.76 CS.reconfigure :pressurize @14:39:58.80 CS.seek 0ml @14:40:16.73 BubblePoint: Intake @39.2psia, Outlet @14.8psia @14:40:16.74 CBV.dial SSV @14:40:17.07 SS.reconfigure :default @14:40:17.11 SS.seek 10ml @14:40:22.80 delay 15 seconds @14:40:37.91 Evacuated: Intake @16.8psia, Outlet @8.3psia @14:40:37.92 SSV.dial :spit,avoiding: :exhaust @14:40:39.24 SS.reconfigure :exhale @14:40:39.28 SS.seek 0ml @14:40:44.50 SS.reconfigure :fast @14:40:44.54 SS.seek 5ml @14:40:47.40 SSV.dial CBV,avoiding: :exhaust @14:40:47.43
CBV.dial SSV @14:40:48.74 CTV.dial :air,avoiding: :intake @14:40:50.08 CSV.dial CTV @14:40:50.12
SS.seek 10ml @14:40:51.11 CSV.dial :air @14:40:51.55 CS.reconfigure :fast @14:40:51.59 CS.seek 0ml @14:40:52.60
SSV.dial :spit,avoiding: :exhaust @14:40:53.93 SS.reconfigure :exhale @14:40:53.97 SS.seek 0ml @14:40:59.03 SS.reconfigure :fast @14:40:59.07 SS.seek 5ml @14:40:59.73 CS.stop @14:41:01.84 SSV.dial CBV,avoiding: :exhaust @14:41:01.88
CBV.dial SSV @14:41:04.17 SSV.dial :spit,avoiding: :exhaust @14:41:05.94 SS.reconfigure :default @14:41:05.99 SS.seek 0ml @14:41:09.31 SS.stop @14:41:09.35 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @14:41:09.36 Intake.select :close @14:41:09.43 CSV.dial :waste @14:41:09.55 SSV.dial CBV,avoiding: :exhaust @14:41:09.59
CBV.dial SSV @14:41:10.21 CS.seek 0ml @14:41:10.27 CSV.dial :air @14:41:10.88
CTV.dial :air @14:41:10.89 delay 15 seconds @14:41:11.12 CS.seek 10ml @14:41:25.91
SSV.dial :spit,avoiding: :exhaust @14:41:27.23 CTV.dial CSV,avoiding: :intake @14:41:27.26
SS.reconfigure :exhale @14:41:27.35 SS.seek 0ml @14:41:27.75 SSV.dial CBV,avoiding: :exhaust @14:41:35.54 CBV.dial between SSV and waste2 @14:41:35.93 CTV.dial CSV,avoiding: :air @14:41:35.95
CSV.dial CTV @14:41:36.54 CS.reconfigure :pressurize @14:41:36.58 CS.seek 0ml @14:41:57.80 BubblePoint: Intake @37.8psia, Outlet @13.7psia @14:41:57.81 CBV.dial SSV @14:41:58.13 SS.reconfigure :default @14:41:58.17 SS.seek 10ml @14:42:03.72 delay 15 seconds @14:42:18.83 Evacuated: Intake @18.4psia, Outlet @11.1psia @14:42:18.84 SSV.dial :spit,avoiding: :exhaust @14:42:20.16 SS.reconfigure :exhale @14:42:20.21 SS.seek 0ml @14:42:25.60 SS.reconfigure :fast @14:42:25.64 SS.seek 5ml @14:42:28.43 SSV.dial CBV,avoiding: :exhaust @14:42:28.45
CBV.dial SSV @14:42:29.77 CTV.dial :air,avoiding: :intake @14:42:31.11 CSV.dial CTV @14:42:31.15
SS.seek 10ml @14:42:32.14 CSV.dial :air @14:42:32.58 CS.reconfigure :fast @14:42:32.62 CS.seek 0ml @14:42:33.60
SSV.dial :spit,avoiding: :exhaust @14:42:34.91 SS.reconfigure :exhale @14:42:34.95 SS.seek 0ml @14:42:37.79 CS.stop @14:42:40.17
SS.reconfigure :fast @14:42:40.21 SS.seek 5ml @14:42:43.06 SSV.dial CBV,avoiding: :exhaust @14:42:43.08
CBV.dial SSV @14:42:45.40 SSV.dial :spit,avoiding: :exhaust @14:42:46.71 SS.reconfigure :default @14:42:46.75 SS.seek 0ml @14:42:50.03 SS.stop @14:42:50.06 Lysing: Adding 2.0ml AqMeOH @14:42:50.08 CBV.dial between CSV and waste1,avoiding: :DBV @14:42:51.56 CTV.dial CSV @14:42:52.87 CSV.dial CSR @14:42:53.66 Pulling 1ml of CSR.AqMeOH @14:42:53.67 CSR.select :AqMeOH @14:42:53.78 CS.reconfigure :default @14:42:53.82 CS.seek 1ml @14:42:57.55 delay 5 seconds @14:43:02.56 CSR.select :air @14:43:02.71 CS.seek 6ml @14:43:16.32 delay 2 seconds @14:43:18.34 CSV.dial :waste2 @14:43:18.77 CS.reconfigure :fast @14:43:18.81 CS.seek 0ml @14:43:33.10 CSV.dial CSR @14:43:33.60 Pulling 2ml of CSR.AqMeOH CSR.select :AqMeOH @14:43:33.71 CS.reconfigure :default @14:43:33.75 CS.seek 2ml @14:43:39.67 delay 5 seconds @14:43:44.69 CSR.select :air @14:43:44.84 CS.seek 5ml @14:43:53.45 delay 2 seconds @14:43:55.46 CSV.dial CTV @14:43:55.91 CS.seek 1ml @14:44:07.24 Before lysing Collection Puck Heater at 17C/38.6psia @14:44:07.26 Heating Puck.from 3, :sh1 in Collection Clamp to 70C for 10:00 @14:44:07.27 CH.seek 70C,8:00 @14:46:06.07 CH.hold 10:00,70C @14:46:06.25 Begun lysing Collection Puck Heater at 68.1C/46.9psia @14:56:06.26 Ended lysing Collection Puck Heater at 70C/45.8psia @14:56:06.28 CS.seek 0ml @14:56:09.66 CTV.dial between spare and air,avoiding: :intake @14:56:10.69 CSV.dial :air @14:56:11.13 CS.seek 5ml @14:56:24.90 CSV.dial CTV @14:56:25.34 delay 2 seconds @14:56:27.36 CSV.dial :air @14:56:27.81 delay 2 seconds @14:56:29.83 CSV.dial CTV @14:56:30.29 delay 2 seconds @14:56:32.30 CSV.dial :air @14:56:32.75 delay 2 seconds @14:56:34.77 CS.seek 0ml @14:56:48.49 Puck @74.6psia after final lysis pressurization @14:56:48.50 Recovering Lysate into Collection syringe @14:56:48.51 CSV.dial CBV @14:56:49.50 CBV.dial CSV @14:56:49.87 CS.seek 5ml @14:57:03.65 delay 5 seconds @14:57:08.67 CTV.dial :air @14:57:09.20 CS.seek 7ml @14:57:15.09 delay 5 seconds @14:57:20.11 CSV.dial :air2 @14:57:20.55 CS.seek 10ml @14:57:29.09 CSV.dial between air and CTV,avoiding: CBV @14:57:30.24 Lysing Complete @14:57:30.30 Storing sh1 puck in Collection Clamp to tube 2 @14:57:30.46 SC.reconfigure :tight @14:57:30.50 SC.seek 2 @14:57:34.05 SC.configure :limp @14:57:34.26 Elbow.reconfigure :tight @14:57:34.30 Elbow.seek 2 @14:57:36.08 Forearm.reconfigure :tight @14:57:36.12 Forearm.seek 2 + 450 counts @14:57:37.17 SE.reconfigure :pick @14:57:37.21 SE.seek :beamBreak @14:57:39.52 SE.configure :default @14:57:39.66 Elbow.reconfigure :limp @14:57:39.71 Forearm.reconfigure :loose @14:57:39.75 Forearm.seek 2 @14:57:39.95 Elbow.hold @14:57:40.01 Elbow.reconfigure :tight @14:57:40.10 SE.seek 18.11 Pucks below Top Plate @14:57:40.63 Forearm.force -20 @14:57:40.66 Forearm.seek 2 + 293 counts @14:57:40.94 Forearm.reconfigure :tight @14:57:40.98 Forearm.seek 2 + 450 counts @14:57:41.68 SE.seek 19.11 Pucks below Top Plate @14:57:42.24 Forearm.reconfigure :loose @14:57:42.28 Forearm.seek :retract @14:57:43.24 Elbow.seek CC @14:57:44.92 Elbow.reconfigure :loose @14:57:45.01 Forearm.seek CC @14:57:45.98 Hand.close @14:57:46.60 CC.seek :open @14:57:51.30 Forearm.seek :retract @14:57:52.25 Elbow.reconfigure :tight @14:57:52.29 Elbow.seek 2 @14:57:54.06 Elbow.reconfigure :limp @14:57:54.10 Forearm.seek 2 @14:57:55.12 Elbow.hold @14:57:55.18 Elbow.reconfigure :tight @14:57:55.23 Hand.open @14:57:55.96 Forearm.force -20 @14:57:55.99 Forearm.seek 2 + 298 counts @14:57:56.27 Forearm.reconfigure :tight @14:57:56.32 Forearm.seek 2 + 450 counts @14:57:56.58 SE.seek :down @14:57:57.70
Forearm.reconfigure :loose @14:57:57.74 Forearm.seek :retract @14:57:57.96 SE.stop @14:57:58.72
Tranferring DA @14:57:58.73 Beginning samplePassC @14:57:58.74 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:57:58.92 Elbow.seek FlushPuck::Garage @14:58:00.76 Elbow.reconfigure :loose @14:58:00.84 Forearm.seek :garage @14:58:01.84 Hand.close @14:58:02.58 Forearm.seek :retract @14:58:03.56 Elbow.reconfigure :tight @14:58:03.61 Elbow.seek CC @14:58:06.66 Elbow.reconfigure :loose @14:58:06.76 Forearm.reconfigure :tight @14:58:06.80 Forearm.seek CC @14:58:08.06 CC.seek :closed @14:58:12.59 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.36 @14:58:12.66 Hand.open @14:58:13.28 Forearm.reconfigure :loose @14:58:13.32 Forearm.seek :retract @14:58:14.29 PSV.dial PSR @14:58:15.67 Pulling 1.5ml of PSR.flush @14:58:15.68 PSR.select :flush @14:58:15.79 PS.seek 1.5ml @14:58:31.88 delay 5 seconds @14:58:36.90 PSV.dial :waste1 @14:58:37.36 PS.seek 0ml @14:58:53.44 PSV.dial PSR @14:58:53.89 PSR.select :air @14:58:54.00 PS.seek 2.5ml @14:59:20.42 PRV.dial CBV,avoiding: :ab2 @14:59:20.43
CBV.dial PRV @14:59:22.11 PSV.dial :mixloop @14:59:23.15 CTV.dial :air,avoiding: :intake @14:59:23.16 PTV.dial :PRVmixing @14:59:24.48 PS.seek 0ml @14:59:50.81 PSV.dial :air @14:59:51.27 PS.seek 2.5ml @15:00:17.69 PSV.dial :mixloop @15:00:18.27 PS.seek 0ml @15:00:44.59 PSV.dial :air @15:00:45.04 PS.seek 2.5ml @15:01:11.81 PSV.dial :mixloop @15:01:12.27 PS.seek 0ml @15:01:38.57 Garaging FlushPuck in Collection Clamp @15:01:38.63 Elbow.reconfigure :tight @15:01:38.68 Elbow.seek CC @15:01:38.96 Elbow.reconfigure :loose @15:01:39.04 Forearm.seek CC @15:01:40.01 Hand.close @15:01:40.59 CC.seek :open @15:01:45.23 Forearm.seek :retract @15:01:46.22 Elbow.reconfigure :tight @15:01:46.26 Elbow.seek :garage @15:01:49.14 Elbow.reconfigure :loose @15:01:49.23 Forearm.reconfigure :tight @15:01:49.27 Forearm.seek :garage @15:01:50.33 Hand.open @15:01:51.02 Forearm.reconfigure :loose @15:01:51.06 Forearm.seek :retract @15:01:52.03 PSV.dial :mixloop @15:01:52.04 PTV.dial :PRVmixing @15:01:52.05 PRV.dial CBV @15:01:52.07
CBV.dial PRV @15:01:52.09 CTV.dial :air,avoiding: :intake @15:01:52.11 Loading lyfil puck from tube 3 into Collection Clamp @15:01:52.33 SC.reconfigure :tight @15:01:52.38 SC.seek 3 @15:01:55.91 SC.configure :limp @15:01:56.10 Elbow.reconfigure :tight @15:01:56.14 Elbow.seek 3 @15:01:58.06 Forearm.reconfigure :tight @15:01:58.11 Forearm.seek 3 + 450 counts @15:01:59.20 SE.reconfigure :pick @15:01:59.25 SE.seek :beamBreak @15:02:01.54 SE.configure :default @15:02:01.68 Elbow.seek 3 @15:02:01.85 Forearm.seek 3 + 300 counts @15:02:02.07 Forearm.reconfigure :loose @15:02:02.11 Hand.close @15:02:02.77 SE.seek :down @15:02:03.02
Forearm.seek :retract @15:02:03.98 Elbow.seek CC @15:02:04.80 SE.stop @15:02:05.64
Elbow.reconfigure :loose @15:02:05.73 Forearm.reconfigure :tight @15:02:05.78 Forearm.seek CC @15:02:07.02 CC.seek :closed @15:02:11.53 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.15 @15:02:11.54 Hand.open @15:02:12.16 Forearm.reconfigure :loose @15:02:12.20 Forearm.seek :retract @15:02:13.14 CTV.dial CSV,avoiding: :intake @15:02:14.49 CSV.dial CTV @15:02:14.80 PS.seek 2.5ml @15:02:41.18 CS.seek 4.5ml @15:02:56.37 delay 8 seconds @15:03:04.39 PSV.dial :air @15:03:04.84 delay 2 seconds @15:03:06.86 PSV.dial :waste2 @15:03:07.58 PS.seek 0ml @15:03:33.97 CBV.dial between PRV and waste1 @15:03:34.29 CSV.dial :air @15:03:34.75 CS.seek 10ml @15:03:49.58 CSV.dial CTV @15:03:50.50 CS.seek 0ml @15:04:16.87 CBV.dial :waste1 @15:04:17.16 delay 5 seconds @15:04:22.19 CTV.dial :air,avoiding: :intake @15:04:23.58 Storing lyfil puck in Collection Clamp to tube 2 @15:04:23.74 SC.reconfigure :tight @15:04:23.79 SC.seek 2 @15:04:27.32 SC.configure :limp @15:04:27.51 Elbow.reconfigure :tight @15:04:27.55 Elbow.seek 2 @15:04:29.34 Forearm.reconfigure :tight @15:04:29.38 Forearm.seek 2 + 450 counts @15:04:30.40 SE.reconfigure :pick @15:04:30.45 SE.seek :beamBreak @15:04:32.29 SE.configure :default @15:04:32.44 Elbow.reconfigure :limp @15:04:32.49 Forearm.reconfigure :loose @15:04:32.53 Forearm.seek 2 @15:04:32.74 Elbow.hold @15:04:32.80 Elbow.reconfigure :tight @15:04:32.89 SE.seek 19.11 Pucks below Top Plate @15:04:33.31 Forearm.force -20 @15:04:33.35 Forearm.seek 2 + 289 counts @15:04:33.62 Forearm.reconfigure :tight @15:04:33.67 Forearm.seek 2 + 450 counts @15:04:33.98 SE.seek 20.11 Pucks below Top Plate @15:04:34.64 Forearm.reconfigure :loose @15:04:34.68 Forearm.seek :retract @15:04:35.57 Elbow.seek CC @15:04:37.28 Elbow.reconfigure :loose @15:04:37.37 Forearm.seek CC @15:04:38.37 Hand.close @15:04:38.96 CC.seek :open @15:04:43.71 Forearm.seek :retract @15:04:44.68 Elbow.reconfigure :tight @15:04:44.73 Elbow.seek 2 @15:04:46.47 Elbow.reconfigure :limp @15:04:46.52 Forearm.seek 2 @15:04:47.46 Elbow.hold @15:04:47.52 Elbow.reconfigure :tight @15:04:47.56 Hand.open @15:04:48.32 Forearm.force -20 @15:04:48.35 Forearm.seek 2 + 320 counts @15:04:48.75 Forearm.reconfigure :tight @15:04:48.80 Forearm.seek 2 + 450 counts @15:04:49.11 SE.seek :down @15:04:50.24
Forearm.reconfigure :loose @15:04:50.29 Forearm.seek :retract @15:04:50.45 SE.stop @15:04:51.18
PSV.dial :air @15:04:51.93 PS.seek 2.5ml @15:05:18.21 delay 1 second @15:05:19.23 PSV.dial :mixloop @15:05:19.67 delay 1 second @15:05:20.68 PSV.dial :air @15:05:21.14 delay 1 second @15:05:22.15 PSV.dial :mixloop @15:05:22.61 delay 1 second @15:05:23.62 PSV.dial :air @15:05:24.20 delay 1 second @15:05:25.22 PS.seek 0ml @15:05:51.56 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @15:05:51.73 Elbow.seek FlushPuck::Garage @15:05:53.58 Elbow.reconfigure :loose @15:05:53.67 Forearm.seek :garage @15:05:54.66 Hand.close @15:05:55.32 Forearm.seek :retract @15:05:56.31 Elbow.reconfigure :tight @15:05:56.82 Elbow.seek PC @15:05:58.08 Elbow.reconfigure :loose @15:05:58.17 Forearm.reconfigure :tight @15:05:58.21 Forearm.seek PC @15:05:59.44 PC.seek :closed @15:06:04.06 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.57 @15:06:04.13 Hand.open @15:06:04.77 Forearm.reconfigure :loose @15:06:04.81 Forearm.seek :retract @15:06:05.76 PRV.dial :air @15:06:06.20 PSV.dial :mixloop @15:06:06.71 PS.seek 0.3ml @15:06:10.59 PSV.dial PSR @15:06:11.81 Pulling 0.4ml of PSR.air @15:06:11.82 PSR.select :air @15:06:11.85 PS.seek 0.7ml @15:06:16.81 delay 3 seconds @15:06:19.82 PSR.select :air @15:06:19.85 PS.seek 2.5ml @15:06:39.09 delay 3 seconds @15:06:42.10 PSV.dial :mixloop @15:06:43.14 PTV.dial :mixing @15:06:43.87 PBV.dial :waste2 @15:06:44.80 PS.seek 0ml @15:07:11.25 Garaging FlushPuck in Processing Clamp @15:07:11.31 Elbow.reconfigure :tight @15:07:11.36 Elbow.seek PC @15:07:11.47 Elbow.reconfigure :loose @15:07:11.56 Forearm.seek PC @15:07:12.49 Hand.close @15:07:13.09 PC.seek :open @15:07:17.82 Forearm.seek :retract @15:07:18.76 Elbow.reconfigure :tight @15:07:18.80 Elbow.seek :garage @15:07:20.16 Elbow.reconfigure :loose @15:07:20.25 Forearm.reconfigure :tight @15:07:20.29 Forearm.seek :garage @15:07:21.32 Hand.open @15:07:22.02 Forearm.reconfigure :loose @15:07:22.06 Forearm.seek :retract @15:07:23.05 Loading da puck from tube 3 into Processing Clamp @15:07:23.26 SC.reconfigure :tight @15:07:23.30 SC.seek 3 @15:07:26.89 SC.configure :limp @15:07:27.08 Elbow.reconfigure :tight @15:07:27.12 Elbow.seek 3 @15:07:28.96 Forearm.reconfigure :tight @15:07:29.00 Forearm.seek 3 + 450 counts @15:07:30.05 SE.reconfigure :pick @15:07:30.10 SE.seek :beamBreak @15:07:32.44 SE.configure :default @15:07:32.58 Elbow.seek 3 @15:07:32.74 Forearm.seek 3 + 300 counts @15:07:32.93 Forearm.reconfigure :loose @15:07:32.97 Hand.close @15:07:33.60 SE.seek :down @15:07:33.78
Forearm.seek :retract @15:07:34.71 Elbow.seek PC @15:07:35.66 SE.stop @15:07:37.24
Elbow.reconfigure :loose @15:07:37.33 Forearm.reconfigure :tight @15:07:37.37 Forearm.seek PC @15:07:38.54 PC.seek :closed @15:07:43.19 Processing Clamp onSpring/offSpring for 16/26 samples, on/off torque 1.60 @15:07:43.20 Hand.open @15:07:43.89 Forearm.reconfigure :loose @15:07:43.93 Forearm.seek :retract @15:07:44.95 Heating Puck.from 3, :da in Processing Clamp to 28C for 2:00:00 @15:07:44.96 PH.seek 28C,5:00 @15:08:21.71 PH.hold 2:00:00,28C @15:08:21.75 delay 1:30 @15:09:51.79 PSR.select :air @15:09:51.81 PSV.dial PSR @15:09:52.90 Pulling 0.5ml of PSR.wash @15:09:52.91 PSR.select :wash @15:09:53.03 PS.seek 0.5ml @15:09:58.99 delay 5 seconds @15:10:04.01 PSR.select :air @15:10:04.13 PS.seek 2ml @15:10:20.21 PTV.dial :puck @15:10:20.94 PSV.dial PTV @15:10:21.71 PBV.dial :waste1 @15:10:23.11 PS.seek 1.5ml @15:10:29.05 delay 3 seconds @15:10:32.07 PBV.dial between PSV and waste1 @15:10:32.44 PS.seek 0ml @15:10:48.62 delay 5:00 @15:15:48.63 recoverPSRreagent08 @15:15:48.64 PTV.dial :puck @15:15:48.65 PBV.dial :waste1 @15:15:49.02 delay 30 seconds @15:16:19.04 PBV.dial between PSV and waste1 @15:16:19.41 PSR.select :air @15:16:19.42 PSV.dial PSR @15:16:20.18 PS.reconfigure :fast @15:16:20.22 PS.seek 2.5ml @15:16:43.92 PSV.dial PTV @15:16:44.66 delay 5 seconds @15:16:49.68 PSV.dial PSR @15:16:50.42 delay 5 seconds @15:16:55.44 PSV.dial PTV @15:16:56.31 delay 5 seconds @15:17:01.33 PSV.dial :air @15:17:02.12 PS.seek 0ml @15:17:25.42 PTV.dial :PRVmixing,via: PRV @15:17:26.73 PSV.dial :mixloop @15:17:27.20 PRV.dial :ab1 @15:17:27.96 PS.reconfigure :slow @15:17:28.00 PS.seek 0.15ml @15:17:31.55 delay 2 seconds @15:17:33.56 CBV.dial PRV @15:17:33.59
PRV.dial CBV @15:17:34.14 PS.seek 0.35ml @15:17:38.76 delay 2 seconds @15:17:40.78 CBV.dial between PRV and PSV @15:17:41.11 PRV.dial :air @15:17:41.55 PS.seek 0.9ml @15:17:53.23 PRV.dial :waste2 @15:17:53.96 PS.seek 0.5ml @15:18:02.58 addPMLReagent08 @15:18:02.59 PSV.dial :air @15:18:03.05 PS.reconfigure :default @15:18:03.09 PS.seek 2ml @15:18:19.26 PSV.dial :PML @15:18:19.72 PTV.dial :mixing @15:18:20.45 PBV.dial :waste1 @15:18:20.87 PS.seek 1.85ml @15:18:23.17 PBV.dial between PSV and waste1 @15:18:23.54 PS.seek 0ml @15:18:43.45 delay 2 seconds @15:18:45.47 Beginning samplePassC @15:18:45.48 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @15:18:45.65 Elbow.reconfigure :tight @15:18:45.69 Elbow.seek FlushPuck::Garage @15:18:47.04 Elbow.reconfigure :loose @15:18:47.18 Forearm.seek :garage @15:18:48.14 Hand.close @15:18:49.31 Forearm.seek :retract @15:18:50.28 Elbow.reconfigure :tight @15:18:50.33 Elbow.seek CC @15:18:53.16 Elbow.reconfigure :loose @15:18:53.25 Forearm.reconfigure :tight @15:18:53.29 Forearm.seek CC @15:18:54.50 CC.seek :closed @15:18:59.01 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.37 @15:18:59.08 Hand.open @15:18:59.76 Forearm.reconfigure :loose @15:18:59.80 Forearm.seek :retract @15:19:00.78 PSV.dial PSR @15:19:01.99 Pulling 1.5ml of PSR.flush @15:19:02.00 PSR.select :flush @15:19:02.11 PS.seek 1.5ml @15:19:18.23 delay 5 seconds @15:19:23.25 PSV.dial :waste1 @15:19:23.74 PS.seek 0ml @15:19:39.82 PSV.dial PSR @15:19:40.28 PSR.select :air @15:19:40.39 PS.seek 2.5ml @15:20:06.77 PRV.dial CBV,avoiding: :ab2 @15:20:06.80
CBV.dial PRV @15:20:08.39 PSV.dial :mixloop @15:20:09.42 CTV.dial :air,avoiding: :intake @15:20:09.43 PTV.dial :PRVmixing @15:20:10.21 PS.seek 0ml @15:20:36.57 PSV.dial :air @15:20:37.03 PS.seek 2.5ml @15:21:03.45 PSV.dial :mixloop @15:21:03.91 PS.seek 0ml @15:21:30.26 PSV.dial :air @15:21:30.72 PS.seek 2.5ml @15:21:57.01 PSV.dial :mixloop @15:21:57.49 PS.seek 0ml @15:22:24.01 Garaging FlushPuck in Collection Clamp @15:22:24.08 Elbow.reconfigure :tight @15:22:24.12 Elbow.seek CC @15:22:24.39 Elbow.reconfigure :loose @15:22:24.48 Forearm.seek CC @15:22:25.43 Hand.close @15:22:26.08 CC.seek :open @15:22:30.68 Forearm.seek :retract @15:22:31.64 Elbow.reconfigure :tight @15:22:31.68 Elbow.seek :garage @15:22:34.64 Elbow.reconfigure :loose @15:22:34.73 Forearm.reconfigure :tight @15:22:34.77 Forearm.seek :garage @15:22:35.73 Hand.open @15:22:36.38 Forearm.reconfigure :loose @15:22:36.42 Forearm.seek :retract @15:22:37.43 delayUntil "15:26:45.46" @15:22:37.46 Whole Cell Archive sampling 1000ml @15:22:37.48 Loading wcr puck from tube 3 into Collection Clamp @15:22:37.92 Elbow.reconfigure :tight @15:22:37.97 Elbow.seek 3 @15:22:39.92 Forearm.reconfigure :tight @15:22:39.97 Forearm.seek 3 + 450 counts @15:22:40.98 SE.reconfigure :pick @15:22:41.04 SE.seek :beamBreak @15:22:43.46 SE.configure :default @15:22:43.61 Elbow.seek 3 @15:22:44.25 Forearm.seek 3 + 300 counts @15:22:44.43 Forearm.reconfigure :loose @15:22:44.48 Hand.close @15:22:45.09 SE.seek :down @15:22:45.28 Forearm.seek :retract @15:22:46.22 Elbow.seek CC @15:22:47.19 SE.stop @15:22:47.87 Elbow.reconfigure :loose @15:22:47.96 Forearm.reconfigure :tight @15:22:48.01 Forearm.seek CC @15:22:49.21 CC.seek :closed @15:22:53.73 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.22 @15:22:53.74 Hand.open @15:22:54.38 Forearm.reconfigure :loose @15:22:54.43 Forearm.seek :retract @15:22:55.37 collectSample 1000ml w/bubble point of 35psi @15:22:55.39 Priming 7ml Intake.select :close @15:22:55.40 Exhaust.select :close @15:22:55.41 SS.hold @15:22:55.61 Calibrating pressure sensors @15:22:55.62 CTV.dial :air @15:22:55.63 SSV.dial :spit,avoiding: :exhaust @15:22:55.65 SS.reconfigure :fast @15:22:55.70 SS.seek 5ml @15:22:58.54 SSV.dial CBV @15:22:58.56 CBV.dial SSV @15:22:59.89 delay 3.8 seconds @15:23:03.82 SSV.dial :spit @15:23:05.13 SS.seek 0ml @15:23:07.76 SSV.dial CBV @15:23:09.10 CTV.dial :intake @15:23:09.87 SSV.dial CBV @15:23:09.89 CBV.dial SSV @15:23:09.91 Allowing 40s for 7.0ml of remaining 7.0ml @15:23:09.92 Intake.select :open @15:23:12.07 Intake @15.2psia, vacuum from 9.7 to 12.7psi @15:23:12.09 SS.reconfigure :inhaling 2.20ml/s @15:23:12.13 SS.seek 7ml @15:23:18.54 Stroke completed -- waiting for pressure to equalize ... @15:23:18.56 SS.reconfigure :exhale @15:23:18.60 SS.hold @15:23:29.78 Intake.select :close @15:23:29.92 SSV.dial :exhaust @15:23:30.67 Exhaust.select :open @15:23:30.78 SS.seek 0ml @15:23:34.91 Exhaust.select :close @15:23:35.02 Sampling 1000.0ml @15:23:35.03 Intake.select :close @15:23:35.04 Exhaust.select :close @15:23:35.05 SS.reconfigure :default @15:23:35.09 SS.hold @15:23:35.28 CTV.dial :intake @15:23:35.30 SSV.dial CBV @15:23:35.32 CBV.dial SSV @15:23:36.09 Allowing 130s for 25.0ml of remaining 1000.0ml @15:23:36.11 Intake.select :open @15:23:38.26 Intake @16.0psia, vacuum from 10.0 to 13.0psi @15:23:38.28 SS.reconfigure :inhaling 2.20ml/s @15:23:38.32 SS.seek 25ml @15:23:43.99 Vacuum too high -- waiting up to 40 seconds @15:23:44.48 SS.reconfigure :exhale @15:23:44.52 SS.hold @15:23:46.42 Intake @14.0psia, vacuum from 8.5 to 11.5psi @15:23:46.44 SS.reconfigure :inhaling 1.98ml/s @15:23:46.48 SS.seek 25ml @15:23:53.74 Vacuum too high -- waiting up to 40 seconds @15:23:53.75 SS.reconfigure :exhale @15:23:53.80 SS.hold @15:24:00.51 Intake @14.6psia, vacuum from 9.1 to 12.1psi @15:24:00.53 SS.reconfigure :inhaling 1.79ml/s @15:24:00.57 SS.seek 25ml @15:24:04.56 Stroke completed -- waiting for pressure to equalize ... @15:24:04.57 SS.reconfigure :exhale @15:24:04.61 SS.hold @15:24:09.38 Intake.select :close @15:24:09.52 SSV.dial :exhaust @15:24:10.29 Exhaust.select :open @15:24:10.40 SS.seek 0ml @15:24:19.09 Exhaust.select :close @15:24:19.20 SSV.dial CBV @15:24:19.22 CBV.dial SSV @15:24:20.00 Allowing 130s for 25.0ml of remaining 975.0ml @15:24:20.01 Intake.select :open @15:24:22.17 Intake @15.9psia, vacuum from 10.0 to 13.0psi @15:24:22.19 SS.reconfigure :inhaling 1.79ml/s @15:24:22.23 SS.seek 25ml @15:24:32.33 Vacuum too high -- waiting up to 40 seconds @15:24:32.35 SS.reconfigure :exhale @15:24:32.39 SS.hold @15:24:35.09 Intake @13.7psia, vacuum from 8.2 to 11.2psi @15:24:35.11 SS.reconfigure :inhaling 1.61ml/s @15:24:35.15 SS.seek 25ml @15:24:43.86 Stroke completed -- waiting for pressure to equalize ... @15:24:43.87 SS.reconfigure :exhale @15:24:43.91 SS.hold @15:24:52.62 Intake.select :close @15:24:52.76 SSV.dial :exhaust @15:24:53.53 Exhaust.select :open @15:24:53.64 SS.seek 0ml @15:25:02.65 Exhaust.select :close @15:25:02.76 SSV.dial CBV @15:25:02.78 CBV.dial SSV @15:25:03.61 Allowing 130s for 25.0ml of remaining 950.0ml @15:25:03.62 Intake.select :open @15:25:05.78 Intake @15.9psia, vacuum from 10.0 to 13.0psi @15:25:05.80 SS.reconfigure :inhaling 1.61ml/s @15:25:05.84 SS.seek 25ml @15:25:16.52 Vacuum too high -- waiting up to 40 seconds @15:25:16.53 SS.reconfigure :exhale @15:25:16.57 SS.hold @15:25:19.10 Intake @13.7psia, vacuum from 8.2 to 11.2psi @15:25:19.12 SS.reconfigure :inhaling 1.45ml/s @15:25:19.17 SS.seek 25ml @15:25:28.17 Stroke completed -- waiting for pressure to equalize ... @15:25:28.18 SS.reconfigure :exhale @15:25:28.22 SS.hold @15:25:35.60 Intake.select :close @15:25:35.74 SSV.dial :exhaust @15:25:36.50 Exhaust.select :open @15:25:36.61 SS.seek 0ml @15:25:45.73 Exhaust.select :close @15:25:45.84 SSV.dial CBV @15:25:45.86 CBV.dial SSV @15:25:46.64 Allowing 130s for 25.0ml of remaining 925.0ml @15:25:46.65 Intake.select :open @15:25:48.81 Intake @15.8psia, vacuum from 10.0 to 13.0psi @15:25:48.83 SS.reconfigure :inhaling 1.45ml/s @15:25:48.87 SS.seek 25ml @15:26:02.36 Vacuum too high -- waiting up to 40 seconds @15:26:02.38 SS.reconfigure :exhale @15:26:02.42 SS.hold @15:26:05.53 Intake @14.0psia, vacuum from 8.5 to 11.5psi @15:26:05.55 SS.reconfigure :inhaling 1.30ml/s @15:26:05.59 SS.seek 25ml @15:26:12.85 Stroke completed -- waiting for pressure to equalize ... @15:26:12.87 SS.reconfigure :exhale @15:26:12.91 SS.hold @15:26:19.31 Intake.select :close @15:26:19.45 SSV.dial :exhaust @15:26:20.21 Exhaust.select :open @15:26:20.33 SS.seek 0ml @15:26:29.42 Exhaust.select :close @15:26:29.52 SSV.dial CBV @15:26:29.55 CBV.dial SSV @15:26:30.31 Allowing 130s for 25.0ml of remaining 900.0ml @15:26:30.32 Intake.select :open @15:26:32.48 Intake @15.8psia, vacuum from 10.0 to 13.0psi @15:26:32.50 SS.reconfigure :inhaling 1.30ml/s @15:26:32.54 SS.seek 25ml @15:26:45.47 recoverPRVreagent08 @15:26:45.48 PTV.dial :mixing @15:26:46.24 PBV.dial :waste1 @15:26:46.58 delay 30 seconds @15:26:48.02 Vacuum too high -- waiting up to 40 seconds @15:26:48.03 SS.reconfigure :exhale @15:26:48.07 SS.hold @15:26:51.14 Intake @14.0psia, vacuum from 8.5 to 11.5psi @15:26:51.16 SS.reconfigure :inhaling 1.17ml/s @15:26:51.21 SS.seek 25ml @15:26:58.49 Stroke completed -- waiting for pressure to equalize ... @15:26:58.50 SS.reconfigure :exhale @15:26:58.55 SS.hold @15:27:05.07 Intake.select :close @15:27:05.22 SSV.dial :exhaust @15:27:05.98 Exhaust.select :open @15:27:06.09 SS.seek 0ml @15:27:15.12 Exhaust.select :close @15:27:15.23 SSV.dial CBV @15:27:15.25 CBV.dial SSV @15:27:16.03 Allowing 130s for 25.0ml of remaining 875.0ml @15:27:16.04 Intake.select :open @15:27:16.60 PBV.dial between PSV and waste1 @15:27:16.96 PSV.dial :air @15:27:17.43 PS.reconfigure :fast @15:27:17.48 PS.seek 2.5ml @15:27:18.20 Intake @15.7psia, vacuum from 10.0 to 13.0psi @15:27:18.21 SS.reconfigure :inhaling 1.17ml/s @15:27:18.26 SS.seek 25ml @15:27:40.74 Vacuum too high -- waiting up to 40 seconds @15:27:40.75 SS.reconfigure :exhale @15:27:40.79 SS.hold @15:27:41.31 PSV.dial :mixloop @15:27:41.79 delay 2 seconds @15:27:43.82 PSV.dial :air @15:27:44.32 delay 2 seconds @15:27:45.08 Intake @14.1psia, vacuum from 8.6 to 11.6psi @15:27:45.10 SS.reconfigure :inhaling 1.06ml/s @15:27:45.14 SS.seek 25ml @15:27:46.33 PSV.dial :mixloop @15:27:46.80 delay 2 seconds @15:27:46.90 Stroke completed -- waiting for pressure to equalize ... @15:27:46.91 SS.reconfigure :exhale @15:27:46.95 SS.hold @15:27:48.87 PSV.dial :air @15:27:49.35 PS.seek 0ml @15:27:51.74 Intake.select :close @15:27:51.88 SSV.dial :exhaust @15:27:52.65 Exhaust.select :open @15:27:52.76 SS.seek 0ml @15:28:01.59 Exhaust.select :close @15:28:01.69 SSV.dial CBV @15:28:01.72 CBV.dial SSV @15:28:02.48 Allowing 130s for 25.0ml of remaining 850.0ml @15:28:02.49 Intake.select :open @15:28:04.65 Intake @15.8psia, vacuum from 10.0 to 13.0psi @15:28:04.67 SS.reconfigure :inhaling 1.06ml/s @15:28:04.71 SS.seek 25ml @15:28:12.78 washCycleDA #1 of 2 @15:28:12.79 PSR.select :air @15:28:12.81 PSV.dial PSR @15:28:14.18 Pulling 0.5ml of PSR.wash @15:28:14.19 PSR.select :wash @15:28:14.31 PS.reconfigure :default @15:28:14.35 PS.seek 0.5ml @15:28:20.35 delay 5 seconds @15:28:25.37 PSR.select :air @15:28:25.48 PS.seek 2ml @15:28:31.10 Stroke completed -- waiting for pressure to equalize ... @15:28:31.11 SS.reconfigure :exhale @15:28:31.16 SS.hold @15:28:41.04 Intake.select :close @15:28:41.18 SSV.dial :exhaust @15:28:41.69 PTV.dial :puck @15:28:42.24 Exhaust.select :open @15:28:42.35 SS.seek 0ml @15:28:42.41 PSV.dial PTV @15:28:43.16 PBV.dial :waste1 @15:28:43.60 PS.seek 1.5ml @15:28:49.56 delay 3 seconds @15:28:51.16 Exhaust.select :close @15:28:51.27 SSV.dial CBV @15:28:51.30 CBV.dial SSV @15:28:52.06 Allowing 130s for 25.0ml of remaining 825.0ml @15:28:52.07 Intake.select :open @15:28:52.58 PBV.dial between PSV and waste1 @15:28:52.97 PS.seek 0ml @15:28:54.23 Intake @15.8psia, vacuum from 10.0 to 13.0psi @15:28:54.24 SS.reconfigure :inhaling 1.06ml/s @15:28:54.29 SS.seek 25ml @15:29:09.13 delay 2:00 @15:29:20.17 Vacuum too high -- waiting up to 40 seconds @15:29:20.19 SS.reconfigure :exhale @15:29:20.23 SS.hold @15:29:24.41 Intake @14.0psia, vacuum from 8.5 to 11.5psi @15:29:24.43 SS.reconfigure :inhaling 0.95ml/s @15:29:24.47 SS.seek 25ml @15:29:25.45 Stroke completed -- waiting for pressure to equalize ... @15:29:25.46 SS.reconfigure :exhale @15:29:25.50 SS.hold @15:29:31.05 Intake.select :close @15:29:31.20 SSV.dial :exhaust @15:29:31.96 Exhaust.select :open @15:29:32.07 SS.seek 0ml @15:29:40.85 Exhaust.select :close @15:29:40.96 SSV.dial CBV @15:29:40.98 CBV.dial SSV @15:29:41.76 Allowing 130s for 25.0ml of remaining 800.0ml @15:29:41.77 Intake.select :open @15:29:43.93 Intake @15.8psia, vacuum from 10.0 to 13.0psi @15:29:43.95 SS.reconfigure :inhaling 0.95ml/s @15:29:43.99 SS.seek 25ml @15:30:12.99 Stroke completed -- waiting for pressure to equalize ... @15:30:13.00 SS.reconfigure :exhale @15:30:13.04 SS.hold @15:30:21.59 Intake.select :close @15:30:21.73 SSV.dial :exhaust @15:30:22.49 Exhaust.select :open @15:30:22.60 SS.seek 0ml @15:30:31.66 Exhaust.select :close @15:30:31.77 SSV.dial CBV @15:30:31.79 CBV.dial SSV @15:30:32.57 Allowing 130s for 25.0ml of remaining 775.0ml @15:30:32.58 Intake.select :open @15:30:34.74 Intake @15.7psia, vacuum from 10.0 to 13.0psi @15:30:34.76 SS.reconfigure :inhaling 0.95ml/s @15:30:34.80 SS.seek 25ml @15:31:03.02 Stroke completed -- waiting for pressure to equalize ... @15:31:03.03 SS.reconfigure :exhale @15:31:03.07 SS.hold @15:31:09.14 recoverPSRreagent08 @15:31:09.16 PTV.dial :puck @15:31:09.17 PBV.dial :waste1 @15:31:09.55 delay 30 seconds @15:31:12.84 Intake.select :close @15:31:12.98 SSV.dial :exhaust @15:31:13.75 Exhaust.select :open @15:31:13.86 SS.seek 0ml @15:31:22.65 Exhaust.select :close @15:31:22.76 SSV.dial CBV @15:31:22.77 CBV.dial SSV @15:31:23.55 Allowing 130s for 25.0ml of remaining 750.0ml @15:31:23.57 Intake.select :open @15:31:25.72 Intake @15.6psia, vacuum from 10.0 to 13.0psi @15:31:25.74 SS.reconfigure :inhaling 0.95ml/s @15:31:25.78 SS.seek 25ml @15:31:39.56 PBV.dial between PSV and waste1 @15:31:39.94 PSR.select :air @15:31:39.96 PSV.dial PSR @15:31:40.72 PS.reconfigure :fast @15:31:40.77 PS.seek 2.5ml @15:31:54.80 Stroke completed -- waiting for pressure to equalize ... @15:31:54.82 SS.reconfigure :exhale @15:31:54.86 SS.hold @15:32:04.71 PSV.dial PTV @15:32:05.48 delay 5 seconds @15:32:05.90 Intake.select :close @15:32:06.05 SSV.dial :exhaust @15:32:06.80 Exhaust.select :open @15:32:06.91 SS.seek 0ml @15:32:10.50 PSV.dial PSR @15:32:11.26 delay 5 seconds @15:32:15.65 Exhaust.select :close @15:32:15.76 SSV.dial CBV @15:32:15.78 CBV.dial SSV @15:32:16.28 PSV.dial PTV @15:32:16.55 Allowing 130s for 25.0ml of remaining 725.0ml @15:32:16.57 Intake.select :open @15:32:17.05 delay 5 seconds @15:32:18.72 Intake @15.5psia, vacuum from 10.0 to 13.0psi @15:32:18.74 SS.reconfigure :inhaling 0.95ml/s @15:32:18.78 SS.seek 25ml @15:32:22.06 PSV.dial :air @15:32:22.83 PS.seek 0ml @15:32:46.12 washCycleDA #2 of 2 @15:32:46.13 PSR.select :air @15:32:46.15 PSV.dial PSR @15:32:47.52 Pulling 0.5ml of PSR.wash @15:32:47.53 PSR.select :wash @15:32:47.64 PS.reconfigure :default @15:32:47.69 Stroke completed -- waiting for pressure to equalize ... @15:32:47.72 SS.reconfigure :exhale @15:32:47.75 PS.seek 0.5ml @15:32:47.83 SS.hold @15:32:53.72 delay 5 seconds @15:32:58.81 PSR.select :air @15:32:58.87 Intake.select :close @15:32:58.93 PS.seek 2ml @15:32:59.02 SSV.dial :exhaust @15:32:59.77 Exhaust.select :open @15:32:59.88 SS.seek 0ml @15:33:08.72 Exhaust.select :close @15:33:08.83 SSV.dial CBV @15:33:08.85 CBV.dial SSV @15:33:10.12 Allowing 130s for 25.0ml of remaining 700.0ml @15:33:10.13 Intake.select :open @15:33:12.29 Intake @15.5psia, vacuum from 10.0 to 13.0psi @15:33:12.31 SS.reconfigure :inhaling 0.95ml/s @15:33:12.35 SS.seek 25ml @15:33:15.12 PTV.dial :puck @15:33:15.13 PSV.dial PTV @15:33:15.87 PBV.dial :waste1 @15:33:16.31 PS.seek 1.5ml @15:33:22.31 delay 3 seconds @15:33:25.33 PBV.dial between PSV and waste1 @15:33:25.71 PS.seek 0ml @15:33:41.24 Stroke completed -- waiting for pressure to equalize ... @15:33:41.25 SS.reconfigure :exhale @15:33:41.29 SS.hold @15:33:41.82 delay 2:00 @15:33:53.85 Intake.select :close @15:33:53.99 SSV.dial :exhaust @15:33:54.75 Exhaust.select :open @15:33:54.86 SS.seek 0ml @15:34:03.67 Exhaust.select :close @15:34:03.78 SSV.dial CBV @15:34:03.80 CBV.dial SSV @15:34:04.58 Allowing 130s for 25.0ml of remaining 675.0ml @15:34:04.60 Intake.select :open @15:34:06.75 Intake @15.5psia, vacuum from 10.0 to 13.0psi @15:34:06.77 SS.reconfigure :inhaling 0.95ml/s @15:34:06.82 SS.seek 25ml @15:34:32.29 Vacuum too high -- waiting up to 40 seconds @15:34:32.30 SS.reconfigure :exhale @15:34:32.35 SS.hold @15:34:38.01 Intake @14.1psia, vacuum from 8.6 to 11.6psi @15:34:38.03 SS.reconfigure :inhaling 0.87ml/s @15:34:38.07 SS.seek 25ml @15:34:42.22 Stroke completed -- waiting for pressure to equalize ... @15:34:42.23 SS.reconfigure :exhale @15:34:42.27 SS.hold @15:34:49.74 Intake.select :close @15:34:49.89 SSV.dial :exhaust @15:34:50.64 Exhaust.select :open @15:34:50.75 SS.seek 0ml @15:34:59.59 Exhaust.select :close @15:34:59.70 SSV.dial CBV @15:34:59.72 CBV.dial SSV @15:35:00.50 Allowing 130s for 25.0ml of remaining 650.0ml @15:35:00.52 Intake.select :open @15:35:02.67 Intake @15.5psia, vacuum from 10.0 to 13.0psi @15:35:02.69 SS.reconfigure :inhaling 0.87ml/s @15:35:02.73 SS.seek 25ml @15:35:34.25 Stroke completed -- waiting for pressure to equalize ... @15:35:34.26 SS.reconfigure :exhale @15:35:34.30 SS.hold @15:35:41.84 recoverPSRreagent08 @15:35:41.85 PTV.dial :puck @15:35:41.86 PBV.dial :waste1 @15:35:42.23 delay 30 seconds @15:35:46.99 Intake.select :close @15:35:47.14 SSV.dial :exhaust @15:35:47.89 Exhaust.select :open @15:35:48.00 SS.seek 0ml @15:35:56.83 Exhaust.select :close @15:35:56.94 SSV.dial CBV @15:35:56.97 CBV.dial SSV @15:35:57.72 Allowing 130s for 25.0ml of remaining 625.0ml @15:35:57.74 Intake.select :open @15:35:59.89 Intake @15.6psia, vacuum from 10.0 to 13.0psi @15:35:59.91 SS.reconfigure :inhaling 0.87ml/s @15:35:59.95 SS.seek 25ml @15:36:12.25 PBV.dial between PSV and waste1 @15:36:12.62 PSR.select :air @15:36:12.64 PSV.dial PSR @15:36:13.42 PS.reconfigure :fast @15:36:13.47 PS.seek 2.5ml @15:36:24.48 Vacuum too high -- waiting up to 40 seconds @15:36:24.49 SS.reconfigure :exhale @15:36:24.53 SS.hold @15:36:30.18 Intake @14.4psia, vacuum from 8.9 to 11.9psi @15:36:30.19 SS.reconfigure :inhaling 0.78ml/s @15:36:30.24 SS.seek 25ml @15:36:37.18 PSV.dial PTV @15:36:37.96 delay 5 seconds @15:36:38.31 Stroke completed -- waiting for pressure to equalize ... @15:36:38.32 SS.reconfigure :exhale @15:36:38.37 SS.hold @15:36:42.98 PSV.dial PSR @15:36:43.74 delay 5 seconds @15:36:46.86 Intake.select :close @15:36:47.00 SSV.dial :exhaust @15:36:47.77 Exhaust.select :open @15:36:47.88 SS.seek 0ml @15:36:48.76 PSV.dial PTV @15:36:49.52 delay 5 seconds @15:36:54.54 PSV.dial :air @15:36:55.42 PS.seek 0ml @15:36:56.90 Exhaust.select :close @15:36:57.01 SSV.dial CBV @15:36:57.03 CBV.dial SSV @15:36:57.80 Allowing 130s for 25.0ml of remaining 600.0ml @15:36:57.81 Intake.select :open @15:36:59.97 Intake @15.5psia, vacuum from 10.0 to 13.0psi @15:36:59.99 SS.reconfigure :inhaling 0.78ml/s @15:37:00.03 SS.seek 25ml @15:37:18.78 getPMLReagent08 @15:37:18.79 PRV.dial :air @15:37:19.24 delay 5 seconds @15:37:24.26 PTV.dial :PRVmixing @15:37:25.59 delay 5 seconds @15:37:30.61 PRV.dial :ab2 @15:37:31.05 PTV.dial :PRVmixing @15:37:31.07 PSV.dial :mixloop @15:37:31.64 PS.reconfigure :default @15:37:31.68 PS.seek 0.3ml @15:37:34.06 Stroke completed -- waiting for pressure to equalize ... @15:37:34.07 SS.reconfigure :exhale @15:37:34.11 SS.hold @15:37:35.57 delay 2 seconds @15:37:37.67 PRV.dial :air @15:37:38.19 PS.seek 0.55ml @15:37:41.49 addPMLReagent08 @15:37:41.50 PSV.dial :air @15:37:41.95 PS.seek 2ml @15:37:48.59 Intake.select :close @15:37:48.73 SSV.dial :exhaust @15:37:49.50 Exhaust.select :open @15:37:49.61 SS.seek 0ml @15:37:57.61 PSV.dial :PML @15:37:58.08 PTV.dial :mixing @15:37:58.45 Exhaust.select :close @15:37:58.56 SSV.dial CBV @15:37:58.58 CBV.dial SSV @15:37:58.83 PBV.dial :waste1 @15:37:59.27 PS.seek 1.85ml @15:37:59.38 Allowing 130s for 25.0ml of remaining 575.0ml @15:37:59.39 Intake.select :open @15:38:01.54 Intake @15.5psia, vacuum from 10.0 to 13.0psi @15:38:01.56 SS.reconfigure :inhaling 0.78ml/s @15:38:01.61 SS.seek 25ml @15:38:01.66 PBV.dial between PSV and waste1 @15:38:02.06 PS.seek 0ml @15:38:21.99 delay 2 seconds @15:38:24.01 delay 8:00 @15:38:32.85 Vacuum too high -- waiting up to 40 seconds @15:38:32.86 SS.reconfigure :exhale @15:38:32.91 SS.hold @15:38:39.44 Intake @14.5psia, vacuum from 9.0 to 12.0psi @15:38:39.46 SS.reconfigure :inhaling 0.70ml/s @15:38:39.51 SS.seek 25ml @15:38:43.75 Stroke completed -- waiting for pressure to equalize ... @15:38:43.76 SS.reconfigure :exhale @15:38:43.80 SS.hold @15:38:52.26 Intake.select :close @15:38:52.40 SSV.dial :exhaust @15:38:53.17 Exhaust.select :open @15:38:53.28 SS.seek 0ml @15:39:02.09 Exhaust.select :close @15:39:02.20 SSV.dial CBV @15:39:02.23 CBV.dial SSV @15:39:02.98 Allowing 130s for 25.0ml of remaining 550.0ml @15:39:03.00 Intake.select :open @15:39:05.15 Intake @15.6psia, vacuum from 10.0 to 13.0psi @15:39:05.17 SS.reconfigure :inhaling 0.70ml/s @15:39:05.21 SS.seek 25ml @15:39:43.23 Stroke completed -- waiting for pressure to equalize ... @15:39:43.24 SS.reconfigure :exhale @15:39:43.28 SS.hold @15:39:59.46 Intake.select :close @15:39:59.60 SSV.dial :exhaust @15:40:00.36 Exhaust.select :open @15:40:00.47 SS.seek 0ml @15:40:09.53 Exhaust.select :close @15:40:09.63 SSV.dial CBV @15:40:09.65 CBV.dial SSV @15:40:10.44 Allowing 130s for 25.0ml of remaining 525.0ml @15:40:10.45 Intake.select :open @15:40:12.60 Intake @15.5psia, vacuum from 10.0 to 13.0psi @15:40:12.62 SS.reconfigure :inhaling 0.70ml/s @15:40:12.66 SS.seek 25ml @15:40:46.97 Vacuum too high -- waiting up to 40 seconds @15:40:46.98 SS.reconfigure :exhale @15:40:47.03 SS.hold @15:40:55.61 Intake @14.7psia, vacuum from 9.2 to 12.2psi @15:40:55.63 SS.reconfigure :inhaling 0.63ml/s @15:40:55.67 SS.seek 25ml @15:40:59.54 Stroke completed -- waiting for pressure to equalize ... @15:40:59.55 SS.reconfigure :exhale @15:40:59.59 SS.hold @15:41:09.37 Intake.select :close @15:41:09.51 SSV.dial :exhaust @15:41:10.28 Exhaust.select :open @15:41:10.39 SS.seek 0ml @15:41:19.18 Exhaust.select :close @15:41:19.29 SSV.dial CBV @15:41:19.31 CBV.dial SSV @15:41:20.09 Allowing 130s for 25.0ml of remaining 500.0ml @15:41:20.10 Intake.select :open @15:41:22.28 Intake @15.6psia, vacuum from 10.0 to 13.0psi @15:41:22.29 SS.reconfigure :inhaling 0.63ml/s @15:41:22.34 SS.seek 25ml @15:42:01.10 Vacuum too high -- waiting up to 40 seconds @15:42:01.11 SS.reconfigure :exhale @15:42:01.15 SS.hold @15:42:08.63 Intake @14.9psia, vacuum from 9.4 to 12.4psi @15:42:08.65 SS.reconfigure :inhaling 0.57ml/s @15:42:08.69 SS.seek 25ml @15:42:12.73 Stroke completed -- waiting for pressure to equalize ... @15:42:12.74 SS.reconfigure :exhale @15:42:12.78 SS.hold @15:42:21.79 Intake.select :close @15:42:21.93 SSV.dial :exhaust @15:42:22.70 Exhaust.select :open @15:42:22.81 SS.seek 0ml @15:42:31.65 Exhaust.select :close @15:42:31.76 SSV.dial CBV @15:42:31.78 CBV.dial SSV @15:42:32.56 Allowing 130s for 25.0ml of remaining 475.0ml @15:42:32.57 Intake.select :open @15:42:34.73 Intake @15.6psia, vacuum from 10.0 to 13.0psi @15:42:34.75 SS.reconfigure :inhaling 0.57ml/s @15:42:34.79 SS.seek 25ml @15:43:20.94 Stroke completed -- waiting for pressure to equalize ... @15:43:20.95 SS.reconfigure :exhale @15:43:21.00 SS.hold @15:43:37.20 Intake.select :close @15:43:37.34 SSV.dial :exhaust @15:43:38.09 Exhaust.select :open @15:43:38.20 SS.seek 0ml @15:43:46.97 Exhaust.select :close @15:43:47.08 SSV.dial CBV @15:43:47.10 CBV.dial SSV @15:43:47.87 Allowing 130s for 25.0ml of remaining 450.0ml @15:43:47.88 Intake.select :open @15:43:50.03 Intake @15.7psia, vacuum from 10.0 to 13.0psi @15:43:50.05 SS.reconfigure :inhaling 0.57ml/s @15:43:50.10 SS.seek 25ml @15:44:27.69 Vacuum too high -- waiting up to 40 seconds @15:44:27.70 SS.reconfigure :exhale @15:44:27.74 SS.hold @15:44:35.21 Intake @14.8psia, vacuum from 9.3 to 12.3psi @15:44:35.23 SS.reconfigure :inhaling 0.51ml/s @15:44:35.28 SS.seek 25ml @15:44:44.71 Stroke completed -- waiting for pressure to equalize ... @15:44:44.72 SS.reconfigure :exhale @15:44:44.76 SS.hold @15:44:57.40 Intake.select :close @15:44:57.54 SSV.dial :exhaust @15:44:58.29 Exhaust.select :open @15:44:58.40 SS.seek 0ml @15:45:07.47 Exhaust.select :close @15:45:07.58 SSV.dial CBV @15:45:07.60 CBV.dial SSV @15:45:08.37 Allowing 130s for 25.0ml of remaining 425.0ml @15:45:08.38 Intake.select :open @15:45:10.53 Intake @15.7psia, vacuum from 10.0 to 13.0psi @15:45:10.55 SS.reconfigure :inhaling 0.51ml/s @15:45:10.59 SS.seek 25ml @15:45:55.68 Vacuum too high -- waiting up to 40 seconds @15:45:56.21 SS.reconfigure :exhale @15:45:56.25 SS.hold @15:46:03.74 Intake @15.1psia, vacuum from 9.6 to 12.6psi @15:46:03.76 SS.reconfigure :inhaling 0.47ml/s @15:46:03.80 SS.seek 25ml @15:46:10.45 Stroke completed -- waiting for pressure to equalize ... @15:46:10.47 SS.reconfigure :exhale @15:46:10.51 SS.hold @15:46:21.56 Intake.select :close @15:46:21.70 SSV.dial :exhaust @15:46:22.46 Exhaust.select :open @15:46:22.57 SS.seek 0ml @15:46:24.02 recoverPRVreagent08 @15:46:24.03 PTV.dial :mixing @15:46:24.05 PBV.dial :waste1 @15:46:24.42 delay 30 seconds @15:46:31.60 Exhaust.select :close @15:46:31.71 SSV.dial CBV @15:46:31.76 CBV.dial SSV @15:46:32.47 Allowing 130s for 25.0ml of remaining 400.0ml @15:46:32.49 Intake.select :open @15:46:34.64 Intake @15.6psia, vacuum from 10.0 to 13.0psi @15:46:34.66 SS.reconfigure :inhaling 0.47ml/s @15:46:34.70 SS.seek 25ml @15:46:54.44 PBV.dial between PSV and waste1 @15:46:54.80 PSV.dial :air @15:46:55.27 PS.reconfigure :fast @15:46:55.31 PS.seek 2.5ml @15:47:19.17 PSV.dial :mixloop @15:47:19.64 delay 2 seconds @15:47:21.65 PSV.dial :air @15:47:22.12 delay 2 seconds @15:47:24.14 PSV.dial :mixloop @15:47:24.61 delay 2 seconds @15:47:26.62 PSV.dial :air @15:47:27.10 PS.seek 0ml @15:47:30.27 Stroke completed -- waiting for pressure to equalize ... @15:47:30.28 SS.reconfigure :exhale @15:47:30.32 SS.hold @15:47:48.55 Intake.select :close @15:47:48.69 SSV.dial :exhaust @15:47:49.44 Exhaust.select :open @15:47:49.55 SS.seek 0ml @15:47:50.26 washCycleDA #1 of 3 @15:47:50.27 PSR.select :air @15:47:50.29 PSV.dial PSR @15:47:51.67 Pulling 0.5ml of PSR.wash @15:47:51.68 PSR.select :wash @15:47:51.80 PS.reconfigure :default @15:47:51.84 PS.seek 0.5ml @15:47:57.76 delay 5 seconds @15:47:58.38 Exhaust.select :close @15:47:58.48 SSV.dial CBV @15:47:58.50 CBV.dial SSV @15:47:59.27 Allowing 130s for 25.0ml of remaining 375.0ml @15:47:59.80 Intake.select :open @15:48:01.96 Intake @15.6psia, vacuum from 10.0 to 13.0psi @15:48:01.98 SS.reconfigure :inhaling 0.47ml/s @15:48:02.02 SS.seek 25ml @15:48:02.78 PSR.select :air @15:48:02.89 PS.seek 2ml @15:48:19.07 PTV.dial :puck @15:48:19.82 PSV.dial PTV @15:48:20.56 PBV.dial :waste1 @15:48:20.98 PS.seek 1.5ml @15:48:26.89 delay 3 seconds @15:48:29.90 PBV.dial between PSV and waste1 @15:48:30.27 PS.seek 0ml @15:48:46.42 delay 2:00 @15:48:55.84 Vacuum too high -- waiting up to 40 seconds @15:48:55.85 SS.reconfigure :exhale @15:48:55.89 SS.hold @15:49:05.67 Intake @15.0psia, vacuum from 9.5 to 12.5psi @15:49:05.69 SS.reconfigure :inhaling 0.43ml/s @15:49:05.73 SS.seek 25ml @15:49:08.11 Stroke completed -- waiting for pressure to equalize ... @15:49:08.12 SS.reconfigure :exhale @15:49:08.16 SS.hold @15:49:19.19 Intake.select :close @15:49:19.34 SSV.dial :exhaust @15:49:20.08 Exhaust.select :open @15:49:20.19 SS.seek 0ml @15:49:29.23 Exhaust.select :close @15:49:29.34 SSV.dial CBV @15:49:29.36 CBV.dial SSV @15:49:30.13 Allowing 130s for 25.0ml of remaining 350.0ml @15:49:30.14 Intake.select :open @15:49:32.29 Intake @15.6psia, vacuum from 10.0 to 13.0psi @15:49:32.31 SS.reconfigure :inhaling 0.43ml/s @15:49:32.35 SS.seek 25ml @15:50:34.06 Stroke completed -- waiting for pressure to equalize ... @15:50:34.07 SS.reconfigure :exhale @15:50:34.11 SS.hold @15:50:46.44 recoverPSRreagent08 @15:50:46.45 PTV.dial :puck @15:50:46.46 PBV.dial :waste1 @15:50:46.80 delay 30 seconds @15:50:54.49 Intake.select :close @15:50:54.63 SSV.dial :exhaust @15:50:55.38 Exhaust.select :open @15:50:55.49 SS.seek 0ml @15:51:04.56 Exhaust.select :close @15:51:04.67 SSV.dial CBV @15:51:04.69 CBV.dial SSV @15:51:05.45 Allowing 130s for 25.0ml of remaining 325.0ml @15:51:05.98 Intake.select :open @15:51:08.15 Intake @15.6psia, vacuum from 10.0 to 13.0psi @15:51:08.17 SS.reconfigure :inhaling 0.43ml/s @15:51:08.22 SS.seek 25ml @15:51:16.82 PBV.dial between PSV and waste1 @15:51:17.18 PSR.select :air @15:51:17.20 PSV.dial PSR @15:51:17.97 PS.reconfigure :fast @15:51:18.01 PS.seek 2.5ml @15:51:41.94 PSV.dial PTV @15:51:42.71 delay 5 seconds @15:51:47.73 PSV.dial PSR @15:51:48.49 delay 5 seconds @15:51:53.51 PSV.dial PTV @15:51:54.27 delay 5 seconds @15:51:59.29 PSV.dial :air @15:52:00.06 PS.seek 0ml @15:52:02.93 Vacuum too high -- waiting up to 40 seconds @15:52:02.94 SS.reconfigure :exhale @15:52:02.98 SS.hold @15:52:14.01 Intake @15.1psia, vacuum from 9.6 to 12.6psi @15:52:14.03 SS.reconfigure :inhaling 0.38ml/s @15:52:14.07 SS.seek 25ml @15:52:22.29 Stroke completed -- waiting for pressure to equalize ... @15:52:22.30 SS.reconfigure :exhale @15:52:22.35 SS.hold @15:52:23.23 washCycleDA #2 of 3 @15:52:23.24 PSR.select :air @15:52:23.25 PSV.dial PSR @15:52:24.62 Pulling 0.5ml of PSR.wash @15:52:24.63 PSR.select :wash @15:52:24.80 PS.reconfigure :default @15:52:24.85 PS.seek 0.5ml @15:52:30.87 delay 5 seconds @15:52:35.88 PSR.select :air @15:52:35.99 PS.seek 2ml @15:52:36.71 Intake.select :close @15:52:36.85 SSV.dial :exhaust @15:52:37.59 Exhaust.select :open @15:52:37.70 SS.seek 0ml @15:52:46.51 Exhaust.select :close @15:52:46.62 SSV.dial CBV @15:52:46.64 CBV.dial SSV @15:52:47.42 Allowing 130s for 25.0ml of remaining 300.0ml @15:52:47.43 Intake.select :open @15:52:49.59 Intake @15.7psia, vacuum from 10.0 to 13.0psi @15:52:49.61 SS.reconfigure :inhaling 0.38ml/s @15:52:49.65 SS.seek 25ml @15:52:52.10 PTV.dial :puck @15:52:52.12 PSV.dial PTV @15:52:52.97 PBV.dial :waste1 @15:52:53.41 PS.seek 1.5ml @15:52:59.35 delay 3 seconds @15:53:02.37 PBV.dial between PSV and waste1 @15:53:02.75 PS.seek 0ml @15:53:19.07 delay 2:00 @15:54:00.32 Stroke completed -- waiting for pressure to equalize ... @15:54:00.33 SS.reconfigure :exhale @15:54:00.38 SS.hold @15:54:23.88 Intake.select :close @15:54:24.02 SSV.dial :exhaust @15:54:24.78 Exhaust.select :open @15:54:24.89 SS.seek 0ml @15:54:33.71 Exhaust.select :close @15:54:33.82 SSV.dial CBV @15:54:33.84 CBV.dial SSV @15:54:34.62 Allowing 130s for 25.0ml of remaining 275.0ml @15:54:34.63 Intake.select :open @15:54:36.79 Intake @15.7psia, vacuum from 10.0 to 13.0psi @15:54:36.81 SS.reconfigure :inhaling 0.38ml/s @15:54:36.85 SS.seek 25ml @15:55:19.09 recoverPSRreagent08 @15:55:19.10 PTV.dial :puck @15:55:19.12 PBV.dial :waste1 @15:55:19.49 delay 30 seconds @15:55:41.95 Vacuum too high -- waiting up to 40 seconds @15:55:41.97 SS.reconfigure :exhale @15:55:42.01 SS.hold @15:55:49.50 PBV.dial between PSV and waste1 @15:55:49.88 PSR.select :air @15:55:49.90 PSV.dial PSR @15:55:50.65 PS.reconfigure :fast @15:55:50.69 PS.seek 2.5ml @15:55:53.16 Intake @15.2psia, vacuum from 9.7 to 12.7psi @15:55:53.18 SS.reconfigure :inhaling 0.34ml/s @15:55:53.22 SS.seek 25ml @15:56:00.44 Stroke completed -- waiting for pressure to equalize ... @15:56:00.45 SS.reconfigure :exhale @15:56:00.50 SS.hold @15:56:14.56 PSV.dial PTV @15:56:15.33 delay 5 seconds @15:56:16.73 Intake.select :close @15:56:16.87 SSV.dial :exhaust @15:56:17.63 Exhaust.select :open @15:56:17.74 SS.seek 0ml @15:56:20.35 PSV.dial PSR @15:56:21.10 delay 5 seconds @15:56:26.12 PSV.dial PTV @15:56:26.77 Exhaust.select :close @15:56:26.88 delay 5 seconds @15:56:26.91 SSV.dial CBV @15:56:26.94 CBV.dial SSV @15:56:27.70 Allowing 130s for 25.0ml of remaining 250.0ml @15:56:27.71 Intake.select :open @15:56:29.86 Intake @15.7psia, vacuum from 10.0 to 13.0psi @15:56:29.88 SS.reconfigure :inhaling 0.34ml/s @15:56:29.92 SS.seek 25ml @15:56:31.89 PSV.dial :air @15:56:32.65 PS.seek 0ml @15:56:55.82 washCycleDA #3 of 3 @15:56:55.83 PSR.select :air @15:56:55.84 PSV.dial PSR @15:56:57.21 Pulling 0.5ml of PSR.wash @15:56:57.22 PSR.select :wash @15:56:57.33 PS.reconfigure :default @15:56:57.38 PS.seek 0.5ml @15:57:03.35 delay 5 seconds @15:57:08.37 PSR.select :air @15:57:09.00 PS.seek 2ml @15:57:25.09 PTV.dial :puck @15:57:25.11 PSV.dial PTV @15:57:25.84 PBV.dial :waste1 @15:57:26.28 PS.seek 1.5ml @15:57:32.22 delay 3 seconds @15:57:35.24 PBV.dial between PSV and waste1 @15:57:35.62 PS.seek 0ml @15:57:51.39 Stroke completed -- waiting for pressure to equalize ... @15:57:51.41 SS.reconfigure :exhale @15:57:51.45 SS.hold @15:57:51.99 delay 2:00 @15:58:17.87 Intake.select :close @15:58:18.01 SSV.dial :exhaust @15:58:18.78 Exhaust.select :open @15:58:18.89 SS.seek 0ml @15:58:27.72 Exhaust.select :close @15:58:27.83 SSV.dial CBV @15:58:27.85 CBV.dial SSV @15:58:28.63 Allowing 130s for 25.0ml of remaining 225.0ml @15:58:28.64 Intake.select :open @15:58:30.80 Intake @15.7psia, vacuum from 10.0 to 13.0psi @15:58:30.82 SS.reconfigure :inhaling 0.34ml/s @15:58:30.86 SS.seek 25ml @15:59:52.02 recoverPSRreagent08 @15:59:52.03 PTV.dial :puck @15:59:52.04 PBV.dial :waste1 @15:59:52.40 delay 30 seconds @15:59:53.88 Stroke completed -- waiting for pressure to equalize ... @15:59:53.89 SS.reconfigure :exhale @15:59:53.93 SS.hold @16:00:22.38 Intake.select :close @16:00:22.43 PBV.dial between PSV and waste1 @16:00:22.53 SSV.dial :exhaust @16:00:22.81 PSR.select :air @16:00:22.83 PSV.dial PSR @16:00:23.28 Exhaust.select :open @16:00:23.39 SS.seek 0ml @16:00:23.60 PS.reconfigure :fast @16:00:23.64 PS.seek 2.5ml @16:00:32.25 Exhaust.select :close @16:00:32.36 SSV.dial CBV @16:00:32.38 CBV.dial SSV @16:00:33.14 Allowing 130s for 25.0ml of remaining 200.0ml @16:00:33.16 Intake.select :open @16:00:35.31 Intake @15.8psia, vacuum from 10.0 to 13.0psi @16:00:35.33 SS.reconfigure :inhaling 0.34ml/s @16:00:35.37 SS.seek 25ml @16:00:47.40 PSV.dial PTV @16:00:48.17 delay 5 seconds @16:00:53.19 PSV.dial PSR @16:00:53.94 delay 5 seconds @16:00:58.95 PSV.dial PTV @16:00:59.82 delay 5 seconds @16:01:04.84 PSV.dial :air @16:01:06.13 PS.seek 0ml @16:01:29.28 Gate.power :camera,:ON @16:01:29.29 Mixing DA Substrates @16:01:29.30 PSR.select :air @16:01:29.32 PSV.dial PSR @16:01:30.84 Pulling 0.25ml of PSR.sub1 @16:01:30.88 PSR.select :sub1 @16:01:31.09 PS.reconfigure :default @16:01:31.21 PS.seek 0.25ml @16:01:34.66 delay 5 seconds @16:01:38.92 Vacuum too high -- waiting up to 40 seconds @16:01:38.93 SS.reconfigure :exhale @16:01:38.97 SS.hold @16:01:39.72 Pulling 0.25ml of PSR.sub2 @16:01:39.73 PSR.select :sub2 @16:01:39.90 PS.seek 0.5ml @16:01:43.34 delay 5 seconds @16:01:48.35 PSR.select :air @16:01:48.47 PS.seek 2ml @16:01:51.64 Intake @15.5psia, vacuum from 10.0 to 13.0psi @16:01:51.66 SS.reconfigure :inhaling 0.31ml/s @16:01:51.70 SS.seek 25ml @16:02:04.65 PTV.dial :puck @16:02:04.66 PSV.dial PTV @16:02:05.40 PBV.dial :waste1 @16:02:05.85 PS.seek 1.5ml @16:02:11.78 delay 3 seconds @16:02:13.19 Stroke completed -- waiting for pressure to equalize ... @16:02:13.20 SS.reconfigure :exhale @16:02:13.24 SS.hold @16:02:14.79 PBV.dial between PSV and waste1 @16:02:15.19 PS.seek 0ml @16:02:31.27 delay 10 seconds @16:02:35.55 Intake.select :close @16:02:35.69 SSV.dial :exhaust @16:02:36.47 Exhaust.select :open @16:02:36.58 SS.seek 0ml @16:02:41.29 recoverPSRreagent08 @16:02:41.34 PTV.dial :puck @16:02:41.35 PBV.dial :waste1 @16:02:41.71 delay 30 seconds @16:02:45.39 Exhaust.select :close @16:02:45.50 SSV.dial CBV @16:02:45.52 CBV.dial SSV @16:02:46.30 Allowing 130s for 25.0ml of remaining 175.0ml @16:02:46.32 Intake.select :open @16:02:48.47 Intake @15.9psia, vacuum from 10.0 to 13.0psi @16:02:48.49 SS.reconfigure :inhaling 0.31ml/s @16:02:48.54 SS.seek 25ml @16:03:11.74 PBV.dial between PSV and waste1 @16:03:12.12 PSR.select :air @16:03:12.14 PSV.dial PSR @16:03:12.91 PS.reconfigure :fast @16:03:12.95 PS.seek 2.5ml @16:03:37.09 PSV.dial PTV @16:03:37.98 delay 5 seconds @16:03:43.00 PSV.dial PSR @16:03:43.75 delay 5 seconds @16:03:48.77 PSV.dial PTV @16:03:49.53 delay 5 seconds @16:03:54.55 PSV.dial :air @16:03:55.44 PS.seek 0ml @16:04:01.54 Vacuum too high -- waiting up to 40 seconds @16:04:01.55 SS.reconfigure :exhale @16:04:01.59 SS.hold @16:04:13.26 Intake @15.3psia, vacuum from 9.8 to 12.8psi @16:04:13.28 SS.reconfigure :inhaling 0.28ml/s @16:04:13.33 SS.seek 25ml @16:04:18.96 Additional air clear @16:04:18.97 PSR.select :air @16:04:18.99 PSV.dial PSR @16:04:20.37 Pulling 2ml of PSR.air @16:04:20.38 PSR.select :air @16:04:20.41 PS.reconfigure :default @16:04:20.45 PS.seek 2ml @16:04:34.70 Stroke completed -- waiting for pressure to equalize ... @16:04:34.71 SS.reconfigure :exhale @16:04:34.75 SS.hold @16:04:41.77 delay 5 seconds @16:04:46.79 PTV.dial :puck @16:04:46.80 PSV.dial PTV @16:04:47.55 PBV.dial :waste1 @16:04:47.99 PS.seek 1.5ml @16:04:53.99 delay 3 seconds @16:04:56.33 SS.reconfigure :default @16:04:56.38 SS.hold @16:04:56.49 Intake.select :close @16:04:56.64 ShallowSampler::Clogged in WCR -- Filtering too Slowly after 850.0ml @16:04:56.65 SSV.dial :exhaust @16:04:57.01 PBV.dial between PSV and waste1 @16:04:57.42 delay 1 second @16:04:57.44 Exhaust.select :open @16:04:57.55 SS.reconfigure :exhale @16:04:57.60 SS.seek 0ml @16:04:58.44 PSV.dial :waste1 @16:04:59.50 PS.seek 0ml @16:05:06.68 Exhaust.select :close @16:05:06.79 Sampled 850.0ml @16:05:06.81 Drying puck before fixing for 20:00 @16:05:06.82 CBV.dial :waste2b @16:05:07.58 delay 1:00 @16:05:15.64 Processing Puck Heater at 28C before imaging @16:05:15.65 PH.coast @16:05:15.77 Imaging da puck in Processing Clamp while storing it to tube 2 @16:05:15.93 SC.reconfigure :tight @16:05:15.98 SC.seek 2 @16:05:19.50 SC.configure :limp @16:05:19.69 Elbow.reconfigure :tight @16:05:19.74 Elbow.seek 2 @16:05:21.50 Forearm.reconfigure :tight @16:05:21.55 Forearm.seek 2 + 450 counts @16:05:22.55 SE.reconfigure :pick @16:05:22.60 SE.seek :beamBreak @16:05:24.38 SE.configure :default @16:05:24.52 Elbow.reconfigure :limp @16:05:24.57 Forearm.reconfigure :loose @16:05:24.61 Forearm.seek 2 @16:05:24.86 Elbow.hold @16:05:24.93 Elbow.reconfigure :tight @16:05:25.02 SE.seek 20.11 Pucks below Top Plate @16:05:25.56 Forearm.force -20 @16:05:25.60 Forearm.seek 2 + 286 counts @16:05:25.87 Forearm.reconfigure :tight @16:05:25.92 Forearm.seek 2 + 450 counts @16:05:26.26 SE.seek 21.11 Pucks below Top Plate @16:05:27.35 Forearm.reconfigure :loose @16:05:27.40 Forearm.seek :retract @16:05:28.37 Elbow.seek PC @16:05:30.84 Elbow.reconfigure :loose @16:05:30.93 Forearm.seek PC @16:05:31.92 Hand.close @16:05:32.54 PC.seek :open @16:05:37.31 Forearm.seek :retract @16:05:38.32 Elbow.reconfigure :tight @16:05:38.37 Elbow.seek 2 @16:05:41.06 Forearm.reconfigure :tight @16:05:41.10 Forearm.seek 2 + 450 counts @16:05:42.04 Hand.open @16:05:42.72 SE.seek 20.76 Pucks below Top Plate @16:05:42.96 SE.reconfigure :seal @16:05:43.00 SE.seek 19.8 Pucks below Top Plate @16:05:43.52 Lights, camera, ACTION! @16:05:43.55 PuckCamera: snap -bin=2,2 -auto /var/log/esp/da23jan3016h875ml.tif @16:06:03.05 Waiting >20 seconds for da to relinquish FlushPuck @16:06:07.61 CSV.dial :air @16:06:07.87 SX HX9/16 (USB:14): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 21.028 seconds /var/log/esp/da23jan3016h875ml.tif: TIFF Upload Complete @16:06:07.89 PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da23jan3016h875ml15s.tif @16:06:08.15 CS.reconfigure :fast @16:06:08.21 CS.seek 10ml @16:06:23.50 SX HX9/16 (USB:14): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15 seconds /var/log/esp/midres/da23jan3016h875ml15s.tif: TIFF Upload Complete @16:06:23.53 PuckCamera: snap -bin=2,2 20.0 /var/log/esp/midres/da23jan3016h875ml20s.tif @16:06:32.91 Pumping wcr puck from tube 3 up to 35psi... @16:06:32.93 CBV.dial SSV @16:06:34.24 CBV.dial between CSV and waste2b @16:06:35.22 CSV.dial between air and CTV @16:06:35.55 CS.seek 7.139ml @16:06:43.07 CTV.dial CSV,avoiding: :air @16:06:43.09 CSV.dial CTV @16:06:43.99 CS.reconfigure :pressurize @16:06:44.15 CS.seek 0ml @16:06:44.25 SX HX9/16 (USB:14): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 20 seconds /var/log/esp/midres/da23jan3016h875ml20s.tif: TIFF Upload Complete @16:06:44.26 Gate.power :camera,:OFF @16:06:44.28 DAfans.email "21.028 sec auto-exposure",Subject: "da23jan3016h875ml images!" @16:06:44.53 Elbow.seek 2 @16:06:44.71 Forearm.seek 2 + 450 counts @16:06:44.79 SE.reconfigure :gently @16:06:44.83 SE.seek 20.52 Pucks below Top Plate @16:06:45.49 Elbow.reconfigure :limp @16:06:45.54 Forearm.reconfigure :loose @16:06:45.58 Forearm.seek 2 @16:06:45.85 Elbow.hold @16:06:45.92 Elbow.reconfigure :tight @16:06:45.98 SE.reconfigure :default @16:06:46.04 SE.seek 20.7 Pucks below Top Plate @16:06:46.43 Forearm.force -20 @16:06:46.47 Forearm.seek 2 + 277 counts @16:06:46.80 Forearm.reconfigure :tight @16:06:46.85 Forearm.seek 2 + 450 counts @16:06:47.11 SE.seek :down @16:06:48.25 Forearm.reconfigure :loose @16:06:48.38 Forearm.seek :retract @16:06:48.51 SE.stop @16:06:49.40 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @16:06:50.72 Elbow.seek FlushPuck::Garage @16:06:52.72 Elbow.reconfigure :loose @16:06:53.00 Forearm.seek :garage @16:06:54.07 Hand.close @16:06:55.05 Forearm.seek :retract @16:06:56.10 Elbow.reconfigure :tight @16:06:56.21 Elbow.seek PC @16:06:57.52 Elbow.reconfigure :loose @16:06:57.77 CBV.dial :waste2b @16:06:57.82 Forearm.reconfigure :tight @16:06:58.01 Forearm.seek PC @16:06:58.26 CS.reconfigure :default @16:06:58.35 CS.seek 0ml @16:06:59.57 PC.seek :closed @16:07:04.41 Processing Clamp onSpring/offSpring for 16/26 samples, on/off torque 1.57 @16:07:04.59 delay 1:00 @16:07:04.64 Hand.open @16:07:05.44 Forearm.reconfigure :loose @16:07:05.51 Forearm.seek :retract @16:07:06.51 PSR.select :air @16:07:06.55 PSV.dial PSR @16:07:07.16 Pulling 0.5ml of PSR.flush @16:07:07.20 PSR.select :flush @16:07:07.37 PS.seek 0.5ml @16:07:13.30 delay 5 seconds @16:07:18.34 PSR.select :air @16:07:18.50 PS.seek 2.5ml @16:07:21.51 upsync @16:07:39.74 delay 1 second @16:07:40.76 PRV.dial :waste2,avoiding: :ab2 @16:07:42.61 PTV.dial :PRVmixing @16:07:43.93 PSV.dial :mixloop @16:07:44.97 PS.seek :empty @16:08:04.64 CTV.dial :air,avoiding: :intake @16:08:05.97 Fix #1 of 2 (with RNAlater) for 10:00 @16:08:05.98 CSV.dial CSR @16:08:06.47 Pulling 2ml of CSR.RNAlater @16:08:06.48 CSR.select :RNAlater @16:08:06.59 CS.seek 2ml @16:08:11.30 PSR.select :air @16:08:11.32 PSV.dial PSR @16:08:12.42 Pulling 0.5ml of PSR.flush @16:08:12.43 PSR.select :flush @16:08:12.50 delay 5 seconds @16:08:12.54 PS.seek 0.5ml @16:08:17.51 CSR.select :air @16:08:17.62 CS.reconfigure :fast @16:08:17.67 CS.seek 10ml @16:08:18.55 delay 5 seconds @16:08:23.57 PSR.select :air @16:08:23.68 PS.seek 2.5ml @16:08:37.24 Pumping wcr puck from tube 3 up to 35psi... @16:08:37.26 CBV.dial SSV @16:08:38.56 CBV.dial between CSV and waste2b @16:08:39.51 CSV.dial between air and CTV @16:08:40.11 CS.seek 7.139ml @16:08:44.89 delay 1 second @16:08:45.91 PBV.dial :waste1 @16:08:46.28 PTV.dial :mixing @16:08:47.05 PSV.dial :mixloop @16:08:47.38 CTV.dial CSV,avoiding: :air @16:08:47.40 CSV.dial CTV @16:08:48.10 PS.seek :empty @16:08:48.80 CS.reconfigure :pressurize @16:08:48.85 CS.seek 0ml @16:09:02.32 delay 10:00 @16:09:14.64 PSV.dial PSR @16:09:15.69 PSR.select :flush @16:09:15.81 PS.seek 1ml @16:09:26.86 PSR.select :air @16:09:26.97 PS.seek 2.5ml @16:09:43.30 PSV.dial PTV @16:09:44.55 PTV.dial :puck @16:09:45.28 PBV.dial :waste1 @16:09:45.30 PS.seek 0ml @16:10:11.64 PSV.dial PSR @16:10:12.39 PSR.select :flush @16:10:12.51 PS.seek 0.5ml @16:10:18.48 PSR.select :air @16:10:18.60 PS.seek 2.5ml @16:10:40.05 PSV.dial PTV @16:10:40.80 PBV.dial PSV @16:10:41.37 PS.seek 0ml @16:11:07.73 PSV.dial PBV @16:11:08.21 PS.seek 2.5ml @16:11:34.58 PSV.dial :waste1 @16:11:35.32 PS.seek 0ml @16:12:01.70 PSV.dial PSR @16:12:02.17 PSR.select :air @16:12:02.20 PS.seek 2.5ml @16:12:28.51 PSV.dial PTV @16:12:29.25 PBV.dial PSV @16:12:29.27 PS.seek 0ml @16:12:55.64 PSV.dial PBV @16:12:56.11 PS.seek 2.5ml @16:13:22.54 PSV.dial :waste1 @16:13:23.30 PS.seek 0ml @16:13:49.60 PSV.dial PSR @16:13:50.07 PSR.select :flush @16:13:50.19 PS.seek 1ml @16:14:01.23 PSR.select :air @16:14:01.34 PS.seek 2.5ml @16:14:17.45 PSV.dial PTV @16:14:18.18 PTV.dial :puck @16:14:18.20 PBV.dial :waste1 @16:14:18.75 PS.seek 0ml @16:14:45.29 PSV.dial PSR @16:14:46.06 PSR.select :flush @16:14:46.17 PS.seek 0.5ml @16:14:52.10 PSR.select :air @16:14:52.22 PS.seek 2.5ml @16:15:13.45 PSV.dial PTV @16:15:14.21 PBV.dial PSV @16:15:14.78 PS.seek 0ml @16:15:41.23 PSV.dial PBV @16:15:41.71 PS.seek 2.5ml @16:16:08.04 PSV.dial :waste1 @16:16:08.78 PS.seek 0ml @16:16:35.07 PSV.dial PSR @16:16:35.54 PSR.select :air @16:16:35.56 PS.seek 2.5ml @16:17:01.94 PSV.dial PTV @16:17:02.68 PBV.dial PSV @16:17:02.70 PS.seek 0ml @16:17:29.07 PSV.dial PBV @16:17:29.55 PS.seek 2.5ml @16:17:55.85 PSV.dial :waste1 @16:17:56.59 PS.seek 0ml @16:18:22.91 Garaging FlushPuck in Processing Clamp @16:18:22.99 Elbow.reconfigure :tight @16:18:23.03 Elbow.seek PC @16:18:23.15 Elbow.reconfigure :loose @16:18:23.24 Forearm.seek PC @16:18:24.17 Hand.close @16:18:24.78 PC.seek :open @16:18:29.49 Forearm.seek :retract @16:18:30.42 Elbow.reconfigure :tight @16:18:30.46 Elbow.seek :garage @16:18:31.82 Elbow.reconfigure :loose @16:18:31.91 Forearm.reconfigure :tight @16:18:31.95 Forearm.seek :garage @16:18:32.98 Hand.open @16:18:33.59 Forearm.reconfigure :loose @16:18:33.63 Forearm.seek :retract @16:19:02.35 CBV.dial :waste2b @16:19:02.67 CS.reconfigure :default @16:19:02.72 CS.seek 0ml @16:19:08.89 delay 1:00 @16:20:08.92 CTV.dial :air,avoiding: :intake @16:20:10.24 Fix #2 of 2 (with RNAlater) for 10:00 @16:20:10.25 CSV.dial CSR @16:20:11.21 Pulling 2ml of CSR.RNAlater @16:20:11.22 CSR.select :RNAlater @16:20:11.33 CS.seek 2ml @16:20:17.48 delay 5 seconds @16:20:22.50 CSR.select :air @16:20:22.61 CS.reconfigure :fast @16:20:22.66 CS.seek 10ml @16:20:42.23 Pumping wcr puck from tube 3 up to 35psi... @16:20:42.25 CBV.dial SSV @16:20:43.55 CBV.dial between CSV and waste2b @16:20:44.49 CSV.dial between air and CTV @16:20:45.08 CS.seek 7.089ml @16:20:52.46 CTV.dial CSV,avoiding: :air @16:20:52.48 CSV.dial CTV @16:20:53.86 CS.reconfigure :pressurize @16:20:53.91 CS.seek 0ml @16:21:12.19 delay 10:00 @16:31:12.21 CBV.dial :waste2b @16:31:12.54 CS.reconfigure :default @16:31:12.58 CS.seek 0ml @16:31:13.56 delay 1:00 @16:32:13.63 CTV.dial :air,avoiding: :intake @16:32:15.00 Storing wcr puck in Collection Clamp to tube 2 @16:32:15.22 SC.reconfigure :tight @16:32:15.26 SC.seek 2 @16:32:15.62 SC.configure :limp @16:32:15.82 Elbow.reconfigure :tight @16:32:15.87 Elbow.seek 2 @16:32:17.71 Forearm.reconfigure :tight @16:32:17.75 Forearm.seek 2 + 450 counts @16:32:18.79 SE.reconfigure :pick @16:32:18.84 SE.seek :beamBreak @16:32:20.50 SE.configure :default @16:32:20.65 Elbow.reconfigure :limp @16:32:20.70 Forearm.reconfigure :loose @16:32:20.74 Forearm.seek 2 @16:32:21.00 Elbow.hold @16:32:21.06 Elbow.reconfigure :tight @16:32:21.16 SE.seek 21.12 Pucks below Top Plate @16:32:21.59 Forearm.force -20 @16:32:21.63 Forearm.seek 2 + 286 counts @16:32:21.91 Forearm.reconfigure :tight @16:32:21.96 Forearm.seek 2 + 450 counts @16:32:22.21 SE.seek 22.12 Pucks below Top Plate @16:32:22.85 Forearm.reconfigure :loose @16:32:22.90 Forearm.seek :retract @16:32:23.85 Elbow.seek CC @16:32:25.46 Elbow.reconfigure :loose @16:32:25.55 Forearm.seek CC @16:32:26.54 Hand.close @16:32:27.14 CC.seek :open @16:32:31.87 Forearm.seek :retract @16:32:32.86 Elbow.reconfigure :tight @16:32:32.91 Elbow.seek 2 @16:32:34.67 Elbow.reconfigure :limp @16:32:34.72 Forearm.seek 2 @16:32:35.72 Elbow.hold @16:32:35.79 Elbow.reconfigure :tight @16:32:35.83 Hand.open @16:32:36.50 Forearm.force -20 @16:32:37.02 Forearm.seek 2 + 295 counts @16:32:37.30 Forearm.reconfigure :tight @16:32:37.35 Forearm.seek 2 + 450 counts @16:32:37.73 SE.seek :down @16:32:38.85 Forearm.reconfigure :loose @16:32:38.90 Forearm.seek :retract @16:32:38.96 SE.stop @16:32:39.90
Starting finalPurge @16:32:39.91 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @16:32:40.09 Elbow.seek FlushPuck::Garage @16:32:41.93 Elbow.reconfigure :loose @16:32:42.03 Forearm.seek :garage @16:32:43.08 Hand.close @16:32:43.76 Forearm.seek :retract @16:32:44.78 Elbow.reconfigure :tight @16:32:44.83 Elbow.seek CC @16:32:47.73 Elbow.reconfigure :loose @16:32:48.65 Forearm.reconfigure :tight @16:32:48.69 Forearm.seek CC @16:32:49.94 CC.seek :closed @16:32:54.45 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.23 @16:32:54.53 Hand.open @16:32:55.20 Forearm.reconfigure :loose @16:32:55.24 Forearm.seek :retract @16:32:56.19 Flushing the Collection Valve::Manifold @16:32:56.31 CSV.dial CSR @16:32:56.79 Pulling 5ml of CSR.flush @16:32:56.80 CSR.select :flush @16:32:56.91 CS.seek 5ml @16:33:10.69 delay 5 seconds @16:33:15.71 CSR.select :air @16:33:15.86 CS.reconfigure :fast @16:33:15.90 CS.seek 7.501ml @16:33:22.62 CSV.dial :waste @16:33:23.89 CS.reconfigure :default @16:33:23.94 CS.seek 0ml @16:33:43.90 Flushing lower manifold's waste1 port @16:33:44.02 CSV.dial :flush @16:33:44.46 CS.reconfigure :fast @16:33:44.50 CS.seek 5ml @16:33:57.31 delay 5 seconds @16:34:02.33 CSV.dial :air @16:34:02.83 CS.seek 7ml @16:34:08.44 CSV.dial CTV @16:34:08.47
CTV.dial CSV @16:34:09.84 CBV.dial :waste1 @16:34:10.61 CS.seek 0ml @16:34:27.12 Flushing lower manifold's waste2 port @16:34:27.24 CSV.dial :flush @16:34:27.97 CS.seek 5ml @16:34:40.59 delay 5 seconds @16:34:45.61 CSV.dial :air @16:34:46.12 CS.seek 7ml @16:34:51.69 CSV.dial CTV @16:34:51.73
CTV.dial CSV @16:34:52.26 CBV.dial :waste2 @16:34:53.30 CS.seek 0ml @16:35:09.84 Flushing lower manifold's waste2b port @16:35:09.96 CSV.dial :flush @16:35:10.68 CS.seek 5ml @16:35:23.28 delay 5 seconds @16:35:28.30 CSV.dial :air @16:35:28.81 CS.seek 7.001ml @16:35:34.28 CSV.dial CTV @16:35:34.30
CTV.dial CSV @16:35:34.73 CBV.dial :waste2b @16:35:35.79 CS.seek 0ml @16:35:52.22 Disinfecting external Intake with kill @16:35:52.33 CSV.dial CSR @16:35:52.82 Pulling 7.999ml of CSR.kill CSR.select :kill @16:35:52.94 CS.seek 8ml @16:36:13.06 delay 5 seconds @16:36:18.08 CSR.select :air @16:36:18.19 CS.seek 10ml @16:36:23.72 CSV.dial CBV @16:36:23.75
CBV.dial CSV @16:36:24.78 CTV.dial :intake @16:36:25.56 Intake.select :open @16:36:25.67 CS.seek 2ml @16:36:44.46 Intake.select :close @16:36:44.56 CTV.dial :air @16:36:45.33 CS.reconfigure :default @16:36:45.37 CS.seek 0ml @16:36:51.27 CSV.dial CSR @16:36:52.33 Pulling 5ml of CSR.flush @16:36:52.34 CSR.select :flush @16:36:52.45 CS.seek 5ml @16:37:06.06 delay 5 seconds @16:37:11.08 CSR.select :air @16:37:11.19 CS.seek 10ml @16:37:24.84 CSV.dial CBV @16:37:25.85 CS.seek 0ml @16:37:52.28 Garaging FlushPuck in Collection Clamp @16:37:52.35 Elbow.reconfigure :tight @16:37:52.39 Elbow.seek CC @16:37:52.78 Elbow.reconfigure :loose @16:37:52.87 Forearm.seek CC @16:37:53.87 Hand.close @16:37:54.51 CC.seek :open @16:37:59.13 Forearm.seek :retract @16:38:00.09 Elbow.reconfigure :tight @16:38:00.13 Elbow.seek :garage @16:38:03.03 Elbow.reconfigure :loose @16:38:03.11 Forearm.reconfigure :tight @16:38:03.16 Forearm.seek :garage @16:38:04.15 Hand.open @16:38:04.82 Forearm.reconfigure :loose @16:38:04.86 Forearm.seek :retract @16:38:05.83 Completed [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] begun 13:53:56 @16:38:05.88 Asleep after completing DA_WCR_13023 assays @16:38:05.90 GoodNews.email "{1 => 33,\n 2 => 23,\n 3 => 10,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@16:33:06, 17% flow, 18.2C, 33% humidity, 14.7psia, 13.715V, 0.502A, 0.490A avg, 6.88W\nSampled 850.0ml",Subject: "Asleep after completing DA_WCR_13023 assays" @16:38:11.36 upsync @13:38:06.33UTC07-Feb-23
ESP2 v3.41 I2C Gateway v3.20 for core bus @13:38:08.91 Configuration for short puck jake 1/11/21 by brent@mbari.org /home/esp/esp2/type/shallow/jake/configure.rb @13:38:12.83 Polling Can every 10:00 @13:38:12.99 Cleanly exited at 13:35:45UTC07-Feb-23 @13:38:13.02 Loading /home/esp/esp2/mission/DA_WCR_020723.rb mission @13:38:13.08 GoodNews.email "Starting DA_WCR_020723 w/pucks in tube 3\n{1 => 33,\n 2 => 23,\n 3 => 10,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@13:38:12, 22% flow, 11.0C, 37% humidity, 14.3psia, 13.778V, 0.278A, 0.266A avg, 3.83W",Subject: "DA_WCR_020723 begun" @13:38:13.12
Preparing to sleep until + 00:00:05 @13:38:13.14 Asleep til + 00:00:05 @13:38:13.16 Continuing at 13:38 @13:38:13.45 Gate.power :core,:OFF @13:38:13.46 Gate.power :raw,:OFF @13:38:13.47 Shutdown @13:38:17.60 GoodNews.email "Then, in tube 3: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2/mission/DA_WCR_020723.rb:14\n{1 => 33,\n 2 => 23,\n 3 => 10,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@13:38:13, 22% flow, 10.9C, 37% humidity, 14.3psia, 13.773V, 0.278A, 0.254A avg, 3.83W",Subject: "Asleep until 13:38" @13:38:18.05
Powering core bus @13:38:19.38 upsync @13:38:22.54 upsync @13:38:23.61
Gate.power :raw,:ON @13:38:24.26 CS.configure :default @13:38:24.35 PS.configure :default @13:38:24.44 SS.configure :default @13:38:24.52 CC.configure :default @13:38:24.61 PC.configure :default @13:38:24.72 Forearm.configure :loose @13:38:24.80 Elbow.configure :loose @13:38:24.91 SC.configure :loose @13:38:25.00 SE.configure :default @13:38:25.04 CH.coast @13:38:25.09 CH.configure :default @13:38:25.12 PH.coast @13:38:25.17 PH.configure :default @13:38:25.20 All dwarf controllers running firmware version 3.85 @13:38:25.31 Valve.reset @13:38:25.55 Intake.select :close @13:38:25.58 Exhaust.select :close @13:38:25.61 CTV.dial :air @13:38:25.72 PSV.dial :air @13:38:25.80 CSV.dial :waste1 @13:38:25.82
Forearm.reconfigure :loose @13:38:25.89 Forearm.home.seek :retract @13:38:26.27 SSV.dial :spit,avoiding: :exhaust @13:38:26.66 Hand.close @13:38:26.68
Elbow.jog 1500 @13:38:27.10 PS.reconfigure :default @13:38:27.14 PS.home.seek 0ml @13:38:27.21 CS.reconfigure :fast @13:38:27.25 SS.reconfigure :default @13:38:27.33 CS.home.seek 0ml @13:38:27.36 SS.home.seek 0ml @13:38:27.46
Elbow.reconfigure :loose @13:38:27.50 Elbow.home.seek :clear @13:38:30.90 SE.reconfigure :default @13:38:30.94 SE.home.seek :down @13:38:31.54 SC.reconfigure :tight @13:38:31.58 SC.home.seek :initial @13:38:35.39 PS.stop @13:38:59.94
SC.configure :limp @13:38:59.99 Forearm.seek :retract @13:39:00.04 Elbow.seek :hammer @13:39:01.10 Forearm.reconfigure :tight @13:39:01.14 Forearm.seek retracted @13:39:01.40 PC.reconfigure :default @13:39:01.41
CC.reconfigure :default @13:39:01.50 CC.home.seek :open @13:39:01.52 PC.home.seek :open @13:39:05.06
Phase [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] @13:39:05.07 Notice.email "At 13:39:04 began in tube 3: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2/mission/DA_WCR_020723.rb:14\n{1 => 33,\n 2 => 23,\n 3 => 10,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@13:39:05, 22% flow, 11.0C, 37% humidity, 14.3psia, 13.773V, 0.260A, 0.248A avg, 3.58W",Subject: "Started DA_WCR_020723 phase initialPurge; da [1000, 1000] #da and wsr are run simultanelously" @13:39:05.09
Starting initialPurge @13:39:05.12 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @13:39:05.18 Hand.open @13:39:05.87 Elbow.reconfigure :tight @13:39:05.91 Elbow.seek FlushPuck::Garage @13:39:07.94 Elbow.reconfigure :loose @13:39:08.03 Forearm.reconfigure :loose @13:39:08.07 Forearm.seek :garage @13:39:09.02 Hand.close @13:39:09.75 Forearm.seek :retract @13:39:10.11 upsync @13:39:10.73
Elbow.reconfigure :tight @13:39:10.78 Elbow.seek CC @13:39:14.00 Elbow.reconfigure :loose @13:39:14.09 Forearm.reconfigure :tight @13:39:14.14 Forearm.seek CC @13:39:15.39 CC.seek :closed @13:39:19.91 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.41 @13:39:19.98 Hand.open @13:39:20.68 Forearm.reconfigure :loose @13:39:20.72 Forearm.seek :retract @13:39:21.70 Flushing the Collection Valve::Manifold @13:39:21.81 CSV.dial CSR @13:39:23.26 Pulling 5ml of CSR.flush @13:39:23.27 CSR.select :flush @13:39:23.38 CS.reconfigure :default @13:39:23.42 CS.seek 5ml @13:39:37.01 delay 5 seconds @13:39:42.02 CSR.select :air @13:39:42.18 CS.reconfigure :fast @13:39:42.22 CS.seek 7.501ml @13:39:48.91 CSV.dial :waste @13:39:50.18 CS.reconfigure :default @13:39:50.22 CS.seek 0ml @13:40:10.29 Purging external Intake @13:40:10.40 CSV.dial :flush @13:40:10.98 CS.reconfigure :fast @13:40:11.02 CS.seek 10ml @13:40:35.41 delay 5 seconds @13:40:40.43 CSV.dial CBV @13:40:40.46
CBV.dial CSV @13:40:41.19 CTV.dial :intake @13:40:42.01 Intake.select :open @13:40:42.11 CS.seek 0ml @13:41:05.75 Intake.select :close @13:41:05.85 CTV.dial :air @13:41:06.65 Flushing upper manifold @13:41:06.76 CSV.dial :flush @13:41:07.50 CS.seek 5ml @13:41:20.16 delay 5 seconds @13:41:25.18 CSV.dial :air @13:41:25.69 CS.seek 7ml @13:41:31.26 CSV.dial CBV @13:41:31.30
CBV.dial CSV @13:41:32.96 CTV.dial :air @13:41:32.98 CS.seek 0ml @13:41:49.88 delay 7 seconds @13:41:56.91 CS.seek 3ml @13:42:04.88 CSV.dial :waste @13:42:05.32 CS.seek 0ml @13:42:13.26 CSV.dial :flush @13:42:13.71 CS.seek 3ml @13:42:21.88 delay 5 seconds @13:42:26.90 CSV.dial :air @13:42:27.39 CS.seek 5ml @13:42:32.91 CSV.dial :air @13:42:32.93 CS.seek 0ml @13:42:45.32 delay 7 seconds @13:42:52.34 CS.seek 3ml @13:43:00.53 CSV.dial :waste @13:43:01.26 CS.seek 0ml @13:43:09.25 Garaging FlushPuck in Collection Clamp @13:43:09.32 Elbow.reconfigure :tight @13:43:09.36 Elbow.seek CC @13:43:09.46 Elbow.reconfigure :loose @13:43:09.55 Forearm.seek CC @13:43:10.51 Hand.close @13:43:11.12 CC.seek :open @13:43:15.77 Forearm.seek :retract @13:43:16.73 Elbow.reconfigure :tight @13:43:16.77 Elbow.seek :garage @13:43:20.03 Elbow.reconfigure :loose @13:43:20.12 Forearm.reconfigure :tight @13:43:20.16 Forearm.seek :garage @13:43:21.16 Hand.open @13:43:22.24 Forearm.reconfigure :loose @13:43:22.28 Forearm.seek :retract @13:43:23.23 da sampling at most 1000ml, wcr at most 1000ml @13:43:23.27 Loading sh1 puck from tube 3 into Collection Clamp @13:43:23.28 DAfans.email "Started da in tube 3 at 13:43:23UTC07-Feb-23\n{1 => 33,\n 2 => 23,\n 3 => 10,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@13:39:05, 22% flow, 11.0C, 37% humidity, 14.3psia, 13.773V, 0.260A, 0.248A avg, 3.58W",Subject: "da sampling at most 1000ml, wcr at most 1000ml" @13:43:23.53
SC.reconfigure :tight @13:43:23.57 SC.seek 3 @13:43:27.18 SC.configure :limp @13:43:27.39 Elbow.reconfigure :tight @13:43:27.44 Elbow.seek 3 @13:43:28.51 upsync @13:43:29.48
Forearm.reconfigure :tight @13:43:29.53 Forearm.seek 3 + 450 counts @13:43:30.54 SE.reconfigure :pick @13:43:30.59 SE.seek :beamBreak @13:43:33.22 SE.configure :default @13:43:33.37 Elbow.seek 3 @13:43:33.54 Forearm.seek 3 + 300 counts @13:43:33.80 Forearm.reconfigure :loose @13:43:33.85 Hand.close @13:43:34.51 SE.seek :down @13:43:34.70
Forearm.seek :retract @13:43:35.67 Elbow.seek CC @13:43:36.67 SE.stop @13:43:37.33
Elbow.reconfigure :loose @13:43:37.42 Forearm.reconfigure :tight @13:43:37.46 Forearm.seek CC @13:43:38.72 CC.seek :closed @13:43:43.22 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.29 @13:43:43.23 Hand.open @13:43:43.93 Forearm.reconfigure :loose @13:43:43.98 Forearm.seek :retract @13:43:44.98 collectSample 1000ml w/bubble point of 24psi @13:43:44.99 Priming 7ml @13:43:45.00 Intake.select :close @13:43:45.01 Exhaust.select :close @13:43:45.02 SS.hold @13:43:45.22 Calibrating pressure and temperature sensors @13:43:45.25 CTV.dial :air @13:43:45.26 SSV.dial :spit,avoiding: :exhaust @13:43:45.28 SS.reconfigure :fast @13:43:45.32 SS.seek 5ml @13:43:47.91 SSV.dial CBV @13:43:47.94
CBV.dial SSV @13:43:49.23 delay 3.8 seconds @13:43:53.15 SSV.dial :spit @13:43:54.46 SS.seek 0ml @13:43:57.12 SSV.dial CBV @13:43:58.42 CTV.dial :intake @13:43:59.21 SSV.dial CBV @13:43:59.23
CBV.dial SSV @13:43:59.24 Allowing 40s for 7.0ml of remaining 7.0ml @13:43:59.25 Intake.select :open @13:44:01.41 Intake @15.3psia, vacuum from 9.8 to 12.8psi @13:44:01.43 SS.reconfigure :inhaling 2.20ml/s @13:44:01.47 SS.seek 7ml @13:44:07.73 Stroke completed -- waiting for pressure to equalize ... @13:44:07.74 SS.reconfigure :exhale @13:44:07.78 SS.hold @13:44:10.17 Intake.select :close @13:44:10.31 SSV.dial :exhaust @13:44:11.06 Exhaust.select :open @13:44:11.17 SS.seek 0ml @13:44:14.93 Exhaust.select :close @13:44:15.04 Sampling 1000.0ml Intake.select :close @13:44:15.05 Exhaust.select :close @13:44:15.06 SS.reconfigure :default @13:44:15.10 SS.hold @13:44:15.29 CTV.dial :intake @13:44:15.31 SSV.dial CBV @13:44:15.36
CBV.dial SSV @13:44:16.08 Allowing 130s for 25.0ml of remaining 1000.0ml @13:44:16.09 Intake.select :open @13:44:18.24 Intake @15.3psia, vacuum from 9.8 to 12.8psi @13:44:18.25 SS.reconfigure :inhaling 2.20ml/s @13:44:18.30 SS.seek 25ml @13:44:26.91 Vacuum too high -- waiting up to 40 seconds @13:44:26.92 SS.reconfigure :exhale @13:44:26.96 SS.hold @13:44:27.68 Intake @12.3psia, vacuum from 6.8 to 9.8psi @13:44:27.69 SS.reconfigure :inhaling 1.98ml/s @13:44:27.74 SS.seek 25ml @13:44:31.10 Vacuum too high -- waiting up to 40 seconds @13:44:31.11 SS.reconfigure :exhale @13:44:31.15 SS.hold @13:44:37.04 Intake @13.8psia, vacuum from 8.3 to 11.3psi @13:44:37.06 SS.reconfigure :inhaling 1.79ml/s @13:44:37.10 SS.seek 25ml @13:44:43.31 Stroke completed -- waiting for pressure to equalize ... @13:44:43.32 SS.reconfigure :exhale @13:44:43.36 SS.hold @13:44:49.75 Intake.select :close @13:44:49.89 SSV.dial :exhaust @13:44:50.65 Exhaust.select :open @13:44:50.76 SS.seek 0ml @13:44:59.29 Exhaust.select :close @13:44:59.39 SSV.dial CBV @13:44:59.42
CBV.dial SSV @13:45:00.18 Allowing 130s for 25.0ml of remaining 975.0ml @13:45:00.20 Intake.select :open @13:45:02.35 Intake @15.3psia, vacuum from 9.8 to 12.8psi @13:45:02.37 SS.reconfigure :inhaling 1.79ml/s @13:45:02.41 SS.seek 25ml @13:45:11.72 Vacuum too high -- waiting up to 40 seconds @13:45:11.73 SS.reconfigure :exhale @13:45:11.77 SS.hold @13:45:14.95 Intake @13.2psia, vacuum from 7.7 to 10.7psi @13:45:14.97 SS.reconfigure :inhaling 1.61ml/s @13:45:15.01 SS.seek 25ml @13:45:20.47 Vacuum too high -- waiting up to 40 seconds @13:45:20.48 SS.reconfigure :exhale @13:45:20.52 SS.hold @13:45:27.17 Intake @14.0psia, vacuum from 8.5 to 11.5psi @13:45:27.18 SS.reconfigure :inhaling 1.45ml/s @13:45:27.23 SS.seek 25ml @13:45:32.35 Stroke completed -- waiting for pressure to equalize ... @13:45:32.36 SS.reconfigure :exhale @13:45:32.40 SS.hold @13:45:37.92 Intake.select :close @13:45:38.06 SSV.dial :exhaust @13:45:38.82 Exhaust.select :open @13:45:38.93 SS.seek 0ml @13:45:47.87 Exhaust.select :close @13:45:47.97 SSV.dial CBV @13:45:47.99
CBV.dial SSV @13:45:48.76 Allowing 130s for 25.0ml of remaining 950.0ml @13:45:48.77 Intake.select :open @13:45:50.92 Intake @15.3psia, vacuum from 9.8 to 12.8psi @13:45:50.94 SS.reconfigure :inhaling 1.45ml/s @13:45:50.98 SS.seek 25ml @13:46:02.31 Vacuum too high -- waiting up to 40 seconds @13:46:02.32 SS.reconfigure :exhale @13:46:02.36 SS.hold @13:46:06.62 Intake @13.6psia, vacuum from 8.1 to 11.1psi @13:46:06.64 SS.reconfigure :inhaling 1.30ml/s @13:46:06.68 SS.seek 25ml @13:46:15.35 Vacuum too high -- waiting up to 40 seconds @13:46:15.36 SS.reconfigure :exhale @13:46:15.40 SS.hold @13:46:23.40 Intake @14.3psia, vacuum from 8.8 to 11.8psi @13:46:23.41 SS.reconfigure :inhaling 1.17ml/s @13:46:23.46 SS.seek 25ml @13:46:25.32 Stroke completed -- waiting for pressure to equalize ... @13:46:25.33 SS.reconfigure :exhale @13:46:25.37 SS.hold @13:46:28.83 Intake.select :close @13:46:28.96 SSV.dial :exhaust @13:46:29.73 Exhaust.select :open @13:46:29.84 SS.seek 0ml @13:46:38.53 Exhaust.select :close @13:46:38.63 SSV.dial CBV @13:46:38.65
CBV.dial SSV @13:46:39.42 Allowing 130s for 25.0ml of remaining 925.0ml @13:46:39.43 Intake.select :open @13:46:41.58 Intake @15.5psia, vacuum from 10.0 to 13.0psi @13:46:41.59 SS.reconfigure :inhaling 1.17ml/s @13:46:41.64 SS.seek 25ml @13:46:56.22 Vacuum too high -- waiting up to 40 seconds @13:46:56.23 SS.reconfigure :exhale @13:46:56.27 SS.hold @13:47:00.46 Intake @13.8psia, vacuum from 8.3 to 11.3psi @13:47:00.47 SS.reconfigure :inhaling 1.06ml/s @13:47:00.52 SS.seek 25ml @13:47:11.43 Stroke completed -- waiting for pressure to equalize ... @13:47:11.44 SS.reconfigure :exhale @13:47:11.48 SS.hold @13:47:24.01 Intake.select :close @13:47:24.14 SSV.dial :exhaust @13:47:24.90 Exhaust.select :open @13:47:25.01 SS.seek 0ml @13:47:33.77 Exhaust.select :close @13:47:33.88 SSV.dial CBV @13:47:33.89
CBV.dial SSV @13:47:34.67 Allowing 130s for 25.0ml of remaining 900.0ml @13:47:34.68 Intake.select :open @13:47:36.83 Intake @15.4psia, vacuum from 9.9 to 12.9psi @13:47:36.84 SS.reconfigure :inhaling 1.06ml/s @13:47:36.89 SS.seek 25ml @13:47:53.28 Vacuum too high -- waiting up to 40 seconds @13:47:53.29 SS.reconfigure :exhale @13:47:53.33 SS.hold @13:47:58.94 Intake @14.2psia, vacuum from 8.7 to 11.7psi @13:47:58.96 SS.reconfigure :inhaling 0.95ml/s @13:47:59.00 SS.seek 25ml @13:48:10.41 Stroke completed -- waiting for pressure to equalize ... @13:48:10.42 SS.reconfigure :exhale @13:48:10.46 SS.hold @13:48:23.07 Intake.select :close @13:48:23.20 SSV.dial :exhaust @13:48:23.96 Exhaust.select :open @13:48:24.07 SS.seek 0ml @13:48:32.76 Exhaust.select :close @13:48:32.86 SSV.dial CBV @13:48:32.88
CBV.dial SSV @13:48:33.65 Allowing 130s for 25.0ml of remaining 875.0ml @13:48:33.66 Intake.select :open @13:48:35.81 Intake @15.4psia, vacuum from 9.9 to 12.9psi @13:48:35.83 SS.reconfigure :inhaling 0.95ml/s @13:48:35.87 SS.seek 25ml @13:48:53.31 Vacuum too high -- waiting up to 40 seconds @13:48:53.32 SS.reconfigure :exhale @13:48:53.36 SS.hold @13:48:59.46 Intake @14.3psia, vacuum from 8.8 to 11.8psi @13:48:59.48 SS.reconfigure :inhaling 0.87ml/s @13:48:59.52 SS.seek 25ml @13:49:12.24 Stroke completed -- waiting for pressure to equalize ... @13:49:12.25 SS.reconfigure :exhale @13:49:12.29 SS.hold @13:49:26.52 Intake.select :close @13:49:26.66 SSV.dial :exhaust @13:49:27.41 Exhaust.select :open @13:49:27.52 SS.seek 0ml @13:49:36.24 Exhaust.select :close @13:49:36.35 SSV.dial CBV @13:49:36.36
CBV.dial SSV @13:49:37.13 Allowing 130s for 25.0ml of remaining 850.0ml @13:49:37.14 Intake.select :open @13:49:39.29 Intake @15.5psia, vacuum from 10.0 to 13.0psi @13:49:39.31 SS.reconfigure :inhaling 0.87ml/s @13:49:39.35 SS.seek 25ml @13:49:58.31 Vacuum too high -- waiting up to 40 seconds @13:49:58.32 SS.reconfigure :exhale @13:49:58.36 SS.hold @13:50:05.79 Intake @14.5psia, vacuum from 9.0 to 12.0psi @13:50:05.81 SS.reconfigure :inhaling 0.78ml/s @13:50:05.85 SS.seek 25ml @13:50:19.88 Stroke completed -- waiting for pressure to equalize ... @13:50:19.89 SS.reconfigure :exhale @13:50:19.93 SS.hold @13:50:36.08 Intake.select :close @13:50:36.22 SSV.dial :exhaust @13:50:36.97 Exhaust.select :open @13:50:37.08 SS.seek 0ml @13:50:45.80 Exhaust.select :close @13:50:45.90 SSV.dial CBV @13:50:45.93
CBV.dial SSV @13:50:46.69 Allowing 130s for 25.0ml of remaining 825.0ml @13:50:46.70 Intake.select :open @13:50:48.85 Intake @15.5psia, vacuum from 10.0 to 13.0psi @13:50:48.87 SS.reconfigure :inhaling 0.78ml/s @13:50:48.91 SS.seek 25ml @13:51:10.18 Vacuum too high -- waiting up to 40 seconds @13:51:10.19 SS.reconfigure :exhale @13:51:10.23 SS.hold @13:51:18.75 Intake @14.7psia, vacuum from 9.2 to 12.2psi @13:51:18.77 SS.reconfigure :inhaling 0.70ml/s @13:51:18.81 SS.seek 25ml @13:51:33.95 Stroke completed -- waiting for pressure to equalize ... @13:51:33.96 SS.reconfigure :exhale @13:51:34.01 SS.hold @13:51:50.65 Intake.select :close @13:51:50.79 SSV.dial :exhaust @13:51:51.57 Exhaust.select :open @13:51:51.68 SS.seek 0ml @13:52:00.42 Exhaust.select :close @13:52:00.53 SSV.dial CBV @13:52:00.58
CBV.dial SSV @13:52:01.30 Allowing 130s for 25.0ml of remaining 800.0ml @13:52:01.31 Intake.select :open @13:52:03.46 Intake @15.5psia, vacuum from 10.0 to 13.0psi @13:52:03.48 SS.reconfigure :inhaling 0.70ml/s @13:52:03.52 SS.seek 25ml @13:52:26.53 Vacuum too high -- waiting up to 40 seconds @13:52:26.54 SS.reconfigure :exhale @13:52:26.58 SS.hold @13:52:35.10 Intake @14.7psia, vacuum from 9.2 to 12.2psi @13:52:35.12 SS.reconfigure :inhaling 0.63ml/s @13:52:35.16 SS.seek 25ml @13:52:52.09 Stroke completed -- waiting for pressure to equalize ... @13:52:52.10 SS.reconfigure :exhale @13:52:52.15 SS.hold @13:53:12.39 Intake.select :close @13:53:12.53 SSV.dial :exhaust @13:53:13.29 Exhaust.select :open @13:53:13.39 SS.seek 0ml @13:53:22.19 Exhaust.select :close @13:53:22.29 SSV.dial CBV @13:53:22.33
CBV.dial SSV @13:53:23.07 Allowing 130s for 25.0ml of remaining 775.0ml Intake.select :open @13:53:25.23 Intake @15.4psia, vacuum from 9.9 to 12.9psi @13:53:25.24 SS.reconfigure :inhaling 0.63ml/s @13:53:25.28 SS.seek 25ml @13:53:48.87 Vacuum too high -- waiting up to 40 seconds @13:53:48.88 SS.reconfigure :exhale @13:53:48.92 SS.hold @13:53:58.66 Intake @14.9psia, vacuum from 9.4 to 12.4psi @13:53:58.68 SS.reconfigure :inhaling 0.57ml/s @13:53:58.72 SS.seek 25ml @13:54:18.98 Stroke completed -- waiting for pressure to equalize ... @13:54:18.99 SS.reconfigure :exhale @13:54:19.03 SS.hold @13:54:41.35 Intake.select :close @13:54:41.49 SSV.dial :exhaust @13:54:42.25 Exhaust.select :open @13:54:42.36 SS.seek 0ml @13:54:51.15 Exhaust.select :close @13:54:51.26 SSV.dial CBV @13:54:51.79
CBV.dial SSV @13:54:52.55 Allowing 130s for 25.0ml of remaining 750.0ml @13:54:52.56 Intake.select :open @13:54:54.71 Intake @15.4psia, vacuum from 9.9 to 12.9psi @13:54:54.73 SS.reconfigure :inhaling 0.57ml/s @13:54:54.77 SS.seek 25ml @13:55:21.59 Vacuum too high -- waiting up to 40 seconds @13:55:21.60 SS.reconfigure :exhale @13:55:21.64 SS.hold @13:55:33.14 Intake @14.9psia, vacuum from 9.4 to 12.4psi @13:55:33.15 SS.reconfigure :inhaling 0.51ml/s @13:55:33.20 SS.seek 25ml @13:55:54.75 Stroke completed -- waiting for pressure to equalize ... @13:55:54.76 SS.reconfigure :exhale @13:55:54.80 SS.hold @13:56:17.10 Intake.select :close @13:56:17.24 SSV.dial :exhaust @13:56:18.00 Exhaust.select :open @13:56:18.10 SS.seek 0ml @13:56:26.87 Exhaust.select :close @13:56:26.97 SSV.dial CBV @13:56:27.00
CBV.dial SSV @13:56:27.76 Allowing 130s for 25.0ml of remaining 725.0ml @13:56:27.77 Intake.select :open @13:56:29.93 Intake @15.4psia, vacuum from 9.9 to 12.9psi @13:56:29.94 SS.reconfigure :inhaling 0.51ml/s @13:56:29.99 SS.seek 25ml @13:56:58.89 Vacuum too high -- waiting up to 40 seconds @13:56:58.90 SS.reconfigure :exhale @13:56:58.94 SS.hold @13:57:10.06 Intake @15.0psia, vacuum from 9.5 to 12.5psi @13:57:10.08 SS.reconfigure :inhaling 0.47ml/s @13:57:10.12 SS.seek 25ml @13:57:34.39 Stroke completed -- waiting for pressure to equalize ... @13:57:34.40 SS.reconfigure :exhale @13:57:34.44 SS.hold @13:58:00.91 Intake.select :close @13:58:01.04 SSV.dial :exhaust @13:58:01.79 Exhaust.select :open @13:58:01.90 SS.seek 0ml @13:58:10.90 Exhaust.select :close @13:58:11.00 SSV.dial CBV @13:58:11.02
CBV.dial SSV @13:58:11.79 Allowing 130s for 25.0ml of remaining 700.0ml @13:58:11.80 Intake.select :open @13:58:13.95 Intake @15.4psia, vacuum from 9.9 to 12.9psi @13:58:13.97 SS.reconfigure :inhaling 0.47ml/s @13:58:14.01 SS.seek 25ml @13:58:46.33 Vacuum too high -- waiting up to 40 seconds @13:58:46.34 SS.reconfigure :exhale @13:58:46.38 SS.hold @13:58:59.21 Intake @14.8psia, vacuum from 9.3 to 12.3psi @13:58:59.23 SS.reconfigure :inhaling 0.43ml/s @13:58:59.27 SS.seek 25ml @13:59:24.96 Stroke completed -- waiting for pressure to equalize ... @13:59:24.97 SS.reconfigure :exhale @13:59:25.01 SS.hold @13:59:53.48 Intake.select :close @13:59:53.61 SSV.dial :exhaust @13:59:54.38 Exhaust.select :open @13:59:54.49 SS.seek 0ml @14:00:03.18 Exhaust.select :close @14:00:03.28 SSV.dial CBV @14:00:03.30
CBV.dial SSV @14:00:04.09 Allowing 130s for 25.0ml of remaining 675.0ml @14:00:04.10 Intake.select :open @14:00:06.25 Intake @15.4psia, vacuum from 9.9 to 12.9psi @14:00:06.27 SS.reconfigure :inhaling 0.43ml/s @14:00:06.31 SS.seek 25ml @14:00:43.33 Vacuum too high -- waiting up to 40 seconds @14:00:43.34 SS.reconfigure :exhale @14:00:43.38 SS.hold @14:00:57.72 Intake @15.1psia, vacuum from 9.6 to 12.6psi @14:00:57.73 SS.reconfigure :inhaling 0.38ml/s @14:00:57.78 SS.seek 25ml @14:01:25.63 Stroke completed -- waiting for pressure to equalize ... @14:01:25.64 SS.reconfigure :exhale @14:01:25.68 SS.hold @14:01:54.09 Intake.select :close @14:01:54.23 SSV.dial :exhaust @14:01:54.99 Exhaust.select :open @14:01:55.10 SS.seek 0ml @14:02:03.88 Exhaust.select :close @14:02:04.46 SSV.dial CBV @14:02:04.48
CBV.dial SSV @14:02:05.26 Allowing 130s for 25.0ml of remaining 650.0ml @14:02:05.27 Intake.select :open @14:02:07.42 Intake @15.4psia, vacuum from 9.9 to 12.9psi @14:02:07.44 SS.reconfigure :inhaling 0.38ml/s @14:02:07.48 SS.seek 25ml @14:02:49.39 Vacuum too high -- waiting up to 40 seconds @14:02:49.40 SS.reconfigure :exhale @14:02:49.44 SS.hold @14:03:03.77 Intake @15.0psia, vacuum from 9.5 to 12.5psi @14:03:03.79 SS.reconfigure :inhaling 0.34ml/s @14:03:03.83 SS.seek 25ml @14:03:34.28 Stroke completed -- waiting for pressure to equalize ... @14:03:34.29 SS.reconfigure :exhale @14:03:34.33 SS.hold @14:04:04.79 Intake.select :close @14:04:04.93 SSV.dial :exhaust @14:04:05.69 Exhaust.select :open @14:04:05.80 SS.seek 0ml @14:04:14.55 Exhaust.select :close @14:04:14.65 SSV.dial CBV @14:04:14.67
CBV.dial SSV @14:04:15.44 Allowing 130s for 25.0ml of remaining 625.0ml @14:04:15.45 Intake.select :open @14:04:17.61 Intake @15.3psia, vacuum from 9.8 to 12.8psi @14:04:17.62 SS.reconfigure :inhaling 0.34ml/s @14:04:17.66 SS.seek 25ml @14:05:20.51 Vacuum too high -- waiting up to 40 seconds @14:05:20.52 SS.reconfigure :exhale @14:05:20.56 SS.hold @14:05:38.80 Intake @14.9psia, vacuum from 9.4 to 12.4psi @14:05:38.81 SS.reconfigure :inhaling 0.31ml/s @14:05:38.86 SS.seek 25ml @14:05:56.30 Stroke completed -- waiting for pressure to equalize ... @14:05:56.31 SS.reconfigure :exhale @14:05:56.35 SS.hold @14:06:23.29 Intake.select :close @14:06:23.42 SSV.dial :exhaust @14:06:24.19 Exhaust.select :open @14:06:24.30 SS.seek 0ml @14:06:33.32 Exhaust.select :close @14:06:33.43 SSV.dial CBV @14:06:33.44
CBV.dial SSV @14:06:34.21 Allowing 130s for 25.0ml of remaining 600.0ml @14:06:34.22 Intake.select :open @14:06:36.38 Intake @15.3psia, vacuum from 9.8 to 12.8psi @14:06:36.40 SS.reconfigure :inhaling 0.31ml/s @14:06:36.44 SS.seek 25ml @14:07:53.79 Vacuum too high -- waiting up to 40 seconds @14:07:53.80 SS.reconfigure :exhale @14:07:53.84 SS.hold @14:08:14.08 Intake @15.2psia, vacuum from 9.7 to 12.7psi @14:08:14.09 SS.reconfigure :inhaling 0.28ml/s @14:08:14.13 SS.seek 25ml @14:08:24.58 Stroke completed -- waiting for pressure to equalize ... @14:08:24.59 SS.reconfigure :exhale @14:08:24.63 SS.hold @14:08:44.23 SS.reconfigure :default @14:08:44.27 SS.hold @14:08:44.37 Intake.select :close @14:08:44.51 ShallowSampler::Clogged in MAIN -- Filtering too Slowly after 425.0ml @14:08:44.52 SSV.dial :exhaust @14:08:45.28 Exhaust.select :open @14:08:45.39 SS.reconfigure :exhale @14:08:45.43 SS.seek 0ml @14:08:54.20 Exhaust.select :close @14:08:54.30 Sampled 425.0ml @14:08:54.32 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @14:08:54.33 Intake.select :close @14:08:54.38 CSV.dial :waste @14:08:54.43 CS.seek 0ml @14:08:54.53 CSV.dial :air @14:08:54.56 SSV.dial CBV,avoiding: :exhaust @14:08:54.60
CBV.dial SSV @14:08:55.32 CS.seek 10ml @14:08:56.50
CTV.dial :air @14:08:57.29 delay 15 seconds @14:09:12.31 SSV.dial :spit,avoiding: :exhaust @14:09:13.65 CTV.dial CSV,avoiding: :intake @14:09:13.69
SS.seek 0ml @14:09:13.79 SSV.dial CBV,avoiding: :exhaust @14:09:19.81 CBV.dial between SSV and waste2 @14:09:20.20 CTV.dial CSV,avoiding: :air @14:09:20.22
CSV.dial CTV @14:09:20.67 CS.reconfigure :pressurize @14:09:20.72 CS.seek 0ml @14:09:39.16 BubblePoint: Intake @38.9psia, Outlet @14.6psia @14:09:39.17 CBV.dial SSV @14:09:39.50 SS.reconfigure :default @14:09:39.54 SS.seek 10ml @14:09:45.11 delay 15 seconds @14:10:00.22 Evacuated: Intake @16.7psia, Outlet @8.9psia @14:10:00.23 SSV.dial :spit,avoiding: :exhaust @14:10:02.02 SS.reconfigure :exhale @14:10:02.06 SS.seek 0ml @14:10:07.07 SS.reconfigure :fast @14:10:07.11 SS.seek 5ml @14:10:10.07 SSV.dial CBV,avoiding: :exhaust @14:10:10.10
CBV.dial SSV @14:10:11.41 CTV.dial :air,avoiding: :intake @14:10:12.78 CSV.dial CTV @14:10:12.81
SS.seek 10ml @14:10:13.81 CSV.dial :air @14:10:14.28 CS.reconfigure :fast @14:10:14.33 CS.seek 0ml @14:10:15.66
SSV.dial :spit,avoiding: :exhaust @14:10:16.97 SS.reconfigure :exhale @14:10:17.02 SS.seek 0ml @14:10:22.05 SS.reconfigure :fast @14:10:22.09 SS.seek 5ml @14:10:22.19 CS.stop @14:10:24.85 SSV.dial CBV,avoiding: :exhaust @14:10:24.87
CBV.dial SSV @14:10:27.20 SSV.dial :spit,avoiding: :exhaust @14:10:28.52 SS.reconfigure :default @14:10:28.56 SS.seek 0ml @14:10:31.67 SS.stop @14:10:31.71 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) Intake.select :close @14:10:31.78 CSV.dial :waste @14:10:31.89 SSV.dial CBV,avoiding: :exhaust @14:10:31.91
CBV.dial SSV @14:10:32.55 CS.seek 0ml @14:10:32.61 CSV.dial :air @14:10:33.25
CTV.dial :air @14:10:33.27 delay 15 seconds @14:10:33.35 CS.seek 10ml @14:10:48.28
SSV.dial :spit,avoiding: :exhaust @14:10:49.62 CTV.dial CSV,avoiding: :intake @14:10:49.64
SS.reconfigure :exhale @14:10:49.74 SS.seek 0ml @14:10:50.14 SSV.dial CBV,avoiding: :exhaust @14:10:57.63 CBV.dial between SSV and waste2 @14:10:58.03 CTV.dial CSV,avoiding: :air @14:10:58.05
CSV.dial CTV @14:10:58.50 CS.reconfigure :pressurize @14:10:58.55 CS.seek 0ml @14:11:20.01 BubblePoint: Intake @37.7psia, Outlet @13.6psia @14:11:20.02 CBV.dial SSV @14:11:20.36 SS.reconfigure :default @14:11:20.40 SS.seek 10ml @14:11:25.92 delay 15 seconds @14:11:41.04 Evacuated: Intake @18.6psia, Outlet @11.0psia @14:11:41.05 SSV.dial :spit,avoiding: :exhaust @14:11:42.36 SS.reconfigure :exhale @14:11:42.41 SS.seek 0ml @14:11:47.69 SS.reconfigure :fast @14:11:47.74 SS.seek 5ml @14:11:50.66 SSV.dial CBV,avoiding: :exhaust @14:11:50.68
CBV.dial SSV @14:11:52.02 CTV.dial :air,avoiding: :intake @14:11:53.37 CSV.dial CTV @14:11:53.41
SS.seek 10ml @14:11:54.41 CSV.dial :air @14:11:54.87 CS.reconfigure :fast @14:11:54.92 CS.seek 0ml @14:11:55.98
SSV.dial :spit,avoiding: :exhaust @14:11:57.30 SS.reconfigure :exhale @14:11:57.34 SS.seek 0ml @14:12:00.04 CS.stop @14:12:02.30
SS.reconfigure :fast @14:12:02.34 SS.seek 5ml @14:12:05.37 SSV.dial CBV,avoiding: :exhaust @14:12:05.40
CBV.dial SSV @14:12:07.72 SSV.dial :spit,avoiding: :exhaust @14:12:09.03 SS.reconfigure :default @14:12:09.08 SS.seek 0ml @14:12:12.09 SS.stop @14:12:12.12 Lysing: Adding 2.0ml AqMeOH @14:12:12.14 CBV.dial between CSV and waste1,avoiding: :DBV @14:12:13.64 CTV.dial CSV @14:12:14.96 CSV.dial CSR @14:12:15.87 Pulling 1ml of CSR.AqMeOH @14:12:15.88 CSR.select :AqMeOH @14:12:15.99 CS.reconfigure :default @14:12:16.03 CS.seek 1ml @14:12:19.48 delay 5 seconds @14:12:24.50 CSR.select :air @14:12:24.65 CS.seek 6ml @14:12:38.26 delay 2 seconds @14:12:40.27 CSV.dial :waste2 @14:12:40.75 CS.reconfigure :fast @14:12:40.79 CS.seek 0ml @14:12:55.35 CSV.dial CSR @14:12:56.30 Pulling 2ml of CSR.AqMeOH @14:12:56.31 CSR.select :AqMeOH @14:12:56.42 CS.reconfigure :default @14:12:56.46 CS.seek 2ml @14:13:02.35 delay 5 seconds @14:13:07.37 CSR.select :air @14:13:07.52 CS.seek 4.999ml @14:13:16.35 delay 2 seconds @14:13:18.37 CSV.dial CTV @14:13:18.83 CS.seek 1ml @14:13:29.95 Before lysing Collection Puck Heater at 10.5C/38.2psia @14:13:29.97 Heating Puck.from 3, :sh1 in Collection Clamp to 70C for 10:00 @14:13:29.99 CH.seek 70C,8:00 @14:15:35.59 CH.hold 10:00,70C @14:15:35.76 Begun lysing Collection Puck Heater at 68.1C/46.9psia @14:25:35.77 Ended lysing Collection Puck Heater at 70C/46.1psia @14:25:35.79 CS.seek 0ml @14:25:39.32 CTV.dial between spare and air,avoiding: :intake @14:25:40.34 CSV.dial :air @14:25:40.79 CS.seek 5ml @14:25:54.37 CSV.dial CTV @14:25:54.81 delay 2 seconds @14:25:56.83 CSV.dial :air @14:25:57.28 delay 2 seconds @14:25:59.30 CSV.dial CTV @14:25:59.73 delay 2 seconds @14:26:01.75 CSV.dial :air @14:26:02.19 delay 2 seconds @14:26:04.21 CS.seek 0ml @14:26:17.89 Puck @75.0psia after final lysis pressurization @14:26:17.90 Recovering Lysate into Collection syringe @14:26:17.91 CSV.dial CBV @14:26:18.92 CBV.dial CSV @14:26:19.29 CS.seek 5ml @14:26:33.09 delay 5 seconds @14:26:38.11 CTV.dial :air @14:26:38.65 CS.seek 7ml @14:26:44.56 delay 5 seconds @14:26:49.58 CSV.dial :air2 @14:26:50.02 CS.seek 10ml @14:26:58.47 CSV.dial between air and CTV,avoiding: CBV @14:26:59.63 Lysing Complete @14:26:59.70 Storing sh1 puck in Collection Clamp to tube 2 @14:26:59.85 SC.reconfigure :tight @14:26:59.89 SC.seek 2 @14:27:03.93 SC.configure :limp @14:27:04.12 Elbow.reconfigure :tight @14:27:04.16 Elbow.seek 2 @14:27:05.98 Forearm.reconfigure :tight @14:27:06.02 Forearm.seek 2 + 450 counts @14:27:07.07 SE.reconfigure :pick @14:27:07.12 SE.seek :beamBreak @14:27:08.73 SE.configure :default @14:27:08.87 Elbow.reconfigure :limp @14:27:08.91 Forearm.reconfigure :loose @14:27:08.96 Forearm.seek 2 @14:27:09.18 Elbow.hold @14:27:09.24 Elbow.reconfigure :tight @14:27:09.33 SE.seek 22.12 Pucks below Top Plate @14:27:09.84 Forearm.force -20 @14:27:09.88 Forearm.seek 2 + 277 counts @14:27:10.15 Forearm.reconfigure :tight @14:27:10.20 Forearm.seek 2 + 450 counts @14:27:10.50 SE.seek 23.12 Pucks below Top Plate @14:27:11.04 Forearm.reconfigure :loose @14:27:11.09 Forearm.seek :retract @14:27:11.97 Elbow.seek CC @14:27:13.74 Elbow.reconfigure :loose @14:27:13.82 Forearm.seek CC @14:27:14.82 Hand.close @14:27:15.47 CC.seek :open @14:27:20.18 Forearm.seek :retract @14:27:21.12 Elbow.reconfigure :tight @14:27:21.16 Elbow.seek 2 @14:27:22.94 Elbow.reconfigure :limp @14:27:23.01 Forearm.seek 2 @14:27:23.98 Elbow.hold @14:27:24.04 Elbow.reconfigure :tight @14:27:24.08 Hand.open @14:27:24.83 Forearm.force -20 @14:27:24.86 Forearm.seek 2 + 303 counts @14:27:25.14 Forearm.reconfigure :tight @14:27:25.19 Forearm.seek 2 + 450 counts @14:27:25.45 SE.seek :down @14:27:26.58
Forearm.reconfigure :loose @14:27:26.63 Forearm.seek :retract @14:27:26.65 SE.stop @14:27:27.62
Tranferring DA @14:27:27.63 Beginning samplePassC @14:27:27.65 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:27:27.83 Elbow.seek FlushPuck::Garage @14:27:29.78 Elbow.reconfigure :loose @14:27:29.87 Forearm.seek :garage @14:27:30.82 Hand.close @14:27:31.57 Forearm.seek :retract @14:27:32.51 Elbow.reconfigure :tight @14:27:32.55 Elbow.seek CC @14:27:35.66 Elbow.reconfigure :loose @14:27:35.74 Forearm.reconfigure :tight @14:27:35.79 Forearm.seek CC @14:27:37.08 CC.seek :closed @14:27:41.61 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.34 @14:27:41.67 Hand.open @14:27:42.40 Forearm.reconfigure :loose @14:27:42.44 Forearm.seek :retract @14:27:43.40 PSV.dial PSR @14:27:45.26 Pulling 1.5ml of PSR.flush @14:27:45.27 PSR.select :flush @14:27:45.39 PS.seek 1.5ml @14:28:01.51 delay 5 seconds @14:28:06.53 PSV.dial :waste1 @14:28:07.01 PS.seek 0ml @14:28:23.13 PSV.dial PSR @14:28:23.60 PSR.select :air @14:28:23.71 PS.seek 2.5ml @14:28:50.14 PRV.dial CBV,avoiding: :ab2 @14:28:50.16
CBV.dial PRV @14:28:51.85 PSV.dial :mixloop @14:28:52.90 CTV.dial :air,avoiding: :intake @14:28:52.91 PTV.dial :PRVmixing @14:28:54.26 PS.seek 0ml @14:29:20.63 PSV.dial :air @14:29:21.22 PS.seek 2.5ml @14:29:47.60 PSV.dial :mixloop @14:29:48.04 PS.seek 0ml @14:30:14.41 PSV.dial :air @14:30:14.87 PS.seek 2.5ml @14:30:41.28 PSV.dial :mixloop @14:30:41.75 PS.seek 0ml @14:31:08.13 Garaging FlushPuck in Collection Clamp @14:31:08.20 Elbow.reconfigure :tight @14:31:08.24 Elbow.seek CC @14:31:08.61 Elbow.reconfigure :loose @14:31:08.69 Forearm.seek CC @14:31:09.74 Hand.close @14:31:10.34 CC.seek :open @14:31:14.98 Forearm.seek :retract @14:31:15.98 Elbow.reconfigure :tight @14:31:16.02 Elbow.seek :garage @14:31:19.12 Elbow.reconfigure :loose @14:31:19.21 Forearm.reconfigure :tight @14:31:19.25 Forearm.seek :garage @14:31:20.26 Hand.open @14:31:20.90 Forearm.reconfigure :loose @14:31:20.94 Forearm.seek :retract @14:31:21.88 PSV.dial :mixloop @14:31:21.90 PTV.dial :PRVmixing @14:31:21.91 PRV.dial CBV @14:31:21.93
CBV.dial PRV @14:31:21.95 CTV.dial :air,avoiding: :intake @14:31:21.96 Loading lyfil puck from tube 3 into Collection Clamp @14:31:22.18 SC.reconfigure :tight @14:31:22.22 SC.seek 3 @14:31:25.72 SC.configure :limp @14:31:25.91 Elbow.reconfigure :tight @14:31:25.95 Elbow.seek 3 @14:31:27.96 Forearm.reconfigure :tight @14:31:28.00 Forearm.seek 3 + 450 counts @14:31:29.05 SE.reconfigure :pick @14:31:29.10 SE.seek :beamBreak @14:31:31.67 SE.configure :default @14:31:31.81 Elbow.seek 3 @14:31:31.98 Forearm.seek 3 + 300 counts @14:31:32.16 Forearm.reconfigure :loose @14:31:32.20 Hand.close @14:31:32.85 SE.seek :down @14:31:33.03
Forearm.seek :retract @14:31:33.98 Elbow.seek CC @14:31:35.09 SE.stop @14:31:35.65
Elbow.reconfigure :loose @14:31:35.75 Forearm.reconfigure :tight @14:31:35.79 Forearm.seek CC @14:31:37.07 CC.seek :closed @14:31:41.57 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.11 @14:31:41.58 Hand.open @14:31:42.27 Forearm.reconfigure :loose @14:31:42.31 Forearm.seek :retract @14:31:43.27 CTV.dial CSV,avoiding: :intake @14:31:44.65 CSV.dial CTV @14:31:44.97 PS.seek 2.5ml @14:32:11.44 CS.seek 4.5ml @14:32:26.34 delay 8 seconds @14:32:34.36 PSV.dial :air @14:32:34.82 delay 2 seconds @14:32:36.83 PSV.dial :waste2 @14:32:37.59 PS.seek 0ml @14:33:03.97 CBV.dial between PRV and waste1 @14:33:04.29 CSV.dial :air @14:33:04.76 CS.seek 10ml @14:33:19.68 CSV.dial CTV @14:33:20.12 CS.seek 0ml @14:33:46.87 CBV.dial :waste1 @14:33:47.16 delay 5 seconds @14:33:52.19 CTV.dial :air,avoiding: :intake @14:33:53.60 Storing lyfil puck in Collection Clamp to tube 2 @14:33:53.76 SC.reconfigure :tight @14:33:53.80 SC.seek 2 @14:33:57.40 SC.configure :limp @14:33:57.58 Elbow.reconfigure :tight @14:33:57.63 Elbow.seek 2 @14:33:59.44 Forearm.reconfigure :tight @14:33:59.49 Forearm.seek 2 + 450 counts @14:34:00.52 SE.reconfigure :pick @14:34:00.57 SE.seek :beamBreak @14:34:02.13 SE.configure :default @14:34:02.27 Elbow.reconfigure :limp @14:34:02.32 Forearm.reconfigure :loose @14:34:02.36 Forearm.seek 2 @14:34:02.60 Elbow.hold @14:34:02.66 Elbow.reconfigure :tight @14:34:02.75 SE.seek 23.11 Pucks below Top Plate @14:34:03.28 Forearm.force -20 @14:34:03.31 Forearm.seek 2 + 285 counts @14:34:03.59 Forearm.reconfigure :tight @14:34:03.64 Forearm.seek 2 + 450 counts @14:34:03.87 SE.seek 24.11 Pucks below Top Plate @14:34:04.42 Forearm.reconfigure :loose @14:34:04.46 Forearm.seek :retract @14:34:05.37 Elbow.seek CC @14:34:07.12 Elbow.reconfigure :loose @14:34:07.21 Forearm.seek CC @14:34:08.19 Hand.close @14:34:08.84 CC.seek :open @14:34:13.55 Forearm.seek :retract @14:34:14.57 Elbow.reconfigure :tight @14:34:14.61 Elbow.seek 2 @14:34:16.39 Elbow.reconfigure :limp @14:34:16.44 Forearm.seek 2 @14:34:17.36 Elbow.hold @14:34:17.42 Elbow.reconfigure :tight @14:34:17.47 Hand.open @14:34:18.20 Forearm.force -20 @14:34:18.23 Forearm.seek 2 + 319 counts @14:34:18.52 Forearm.reconfigure :tight @14:34:18.56 Forearm.seek 2 + 450 counts @14:34:18.80 SE.seek :down @14:34:19.95 SE.stop @14:34:20.52
Forearm.reconfigure :loose @14:34:20.61 Forearm.seek :retract @14:34:21.54 PSV.dial :air @14:34:22.43 PS.seek 2.5ml @14:34:48.84 delay 1 second @14:34:49.86 PSV.dial :mixloop @14:34:50.33 delay 1 second @14:34:51.34 PSV.dial :air @14:34:51.94 delay 1 second @14:34:52.95 PSV.dial :mixloop @14:34:53.42 delay 1 second @14:34:54.44 PSV.dial :air @14:34:54.89 delay 1 second @14:34:55.91 PS.seek 0ml @14:35:22.22 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @14:35:22.39 Elbow.seek FlushPuck::Garage @14:35:24.31 Elbow.reconfigure :loose @14:35:24.40 Forearm.seek :garage @14:35:25.41 Hand.close @14:35:26.07 Forearm.seek :retract @14:35:27.02 Elbow.reconfigure :tight @14:35:27.07 Elbow.seek PC @14:35:28.36 Elbow.reconfigure :loose @14:35:28.44 Forearm.reconfigure :tight @14:35:28.48 Forearm.seek PC @14:35:29.73 PC.seek :closed @14:35:34.34 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.58 @14:35:34.41 Hand.open @14:35:35.02 Forearm.reconfigure :loose @14:35:35.06 Forearm.seek :retract @14:35:36.07 PRV.dial :air @14:35:36.53 PSV.dial :mixloop @14:35:37.04 PS.seek 0.3ml @14:35:40.94 PSV.dial PSR @14:35:42.16 Pulling 0.4ml of PSR.air @14:35:42.17 PSR.select :air @14:35:42.19 PS.seek 0.7ml @14:35:47.09 delay 3 seconds @14:35:50.11 PSR.select :air @14:35:50.13 PS.seek 2.5ml @14:36:09.28 delay 3 seconds @14:36:12.30 PSV.dial :mixloop @14:36:13.34 PTV.dial :mixing @14:36:14.11 PBV.dial :waste2 @14:36:15.07 PS.seek 0ml @14:36:41.47 Garaging FlushPuck in Processing Clamp @14:36:41.54 Elbow.reconfigure :tight @14:36:41.58 Elbow.seek PC @14:36:41.68 Elbow.reconfigure :loose @14:36:41.77 Forearm.seek PC @14:36:42.70 Hand.close @14:36:43.34 PC.seek :open @14:36:48.04 Forearm.seek :retract @14:36:49.02 Elbow.reconfigure :tight @14:36:49.06 Elbow.seek :garage @14:36:50.43 Elbow.reconfigure :loose @14:36:50.52 Forearm.reconfigure :tight @14:36:50.56 Forearm.seek :garage @14:36:51.58 Hand.open @14:36:52.26 Forearm.reconfigure :loose @14:36:52.31 Forearm.seek :retract @14:36:53.26 Loading da puck from tube 3 into Processing Clamp @14:36:53.47 SC.reconfigure :tight @14:36:53.52 SC.seek 3 @14:36:57.03 SC.configure :limp @14:36:57.22 Elbow.reconfigure :tight @14:36:57.26 Elbow.seek 3 @14:36:59.21 Forearm.reconfigure :tight @14:36:59.25 Forearm.seek 3 + 450 counts @14:37:00.28 SE.reconfigure :pick @14:37:00.33 SE.seek :beamBreak @14:37:03.49 SE.configure :default @14:37:03.63 Elbow.seek 3 @14:37:03.80 Forearm.seek 3 + 300 counts @14:37:03.94 Forearm.reconfigure :loose @14:37:03.98 Hand.close @14:37:04.60 SE.seek :down @14:37:04.78
Forearm.seek :retract @14:37:05.71 Elbow.seek PC @14:37:06.93 SE.stop @14:37:08.45
Elbow.reconfigure :loose @14:37:08.54 Forearm.reconfigure :tight @14:37:08.58 Forearm.seek PC @14:37:09.79 PC.seek :closed @14:37:14.39 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.62 @14:37:14.40 Hand.open @14:37:15.02 Forearm.reconfigure :loose @14:37:15.06 Forearm.seek :retract @14:37:16.09 Heating Puck.from 3, :da in Processing Clamp to 28C for 2:00:00 @14:37:16.11 PH.seek 28C,5:00 @14:38:01.17 PH.hold 2:00:00,28C @14:38:01.21 delay 1:30 @14:39:31.25 PSR.select :air @14:39:31.27 PSV.dial PSR @14:39:32.38 Pulling 0.5ml of PSR.wash @14:39:32.39 PSR.select :wash @14:39:32.50 PS.seek 0.5ml @14:39:38.50 delay 5 seconds @14:39:43.52 PSR.select :air @14:39:43.63 PS.seek 2ml @14:39:59.68 PTV.dial :puck @14:40:00.42 PSV.dial PTV @14:40:01.18 PBV.dial :waste1 @14:40:02.60 PS.seek 1.5ml @14:40:08.50 delay 3 seconds @14:40:11.51 PBV.dial between PSV and waste1 @14:40:11.88 PS.seek 0ml @14:40:28.06 delay 5:00 @14:45:28.08 recoverPSRreagent08 @14:45:28.09 PTV.dial :puck @14:45:28.10 PBV.dial :waste1 @14:45:28.46 delay 30 seconds @14:45:58.48 PBV.dial between PSV and waste1 @14:45:58.85 PSR.select :air @14:45:58.87 PSV.dial PSR @14:45:59.64 PS.reconfigure :fast @14:45:59.68 PS.seek 2.5ml @14:46:23.52 PSV.dial PTV @14:46:24.29 delay 5 seconds @14:46:29.31 PSV.dial PSR @14:46:30.07 delay 5 seconds @14:46:35.09 PSV.dial PTV @14:46:35.85 delay 5 seconds @14:46:40.87 PSV.dial :air @14:46:41.76 PS.seek 0ml @14:47:04.71 PTV.dial :PRVmixing,via: PRV @14:47:06.04 PSV.dial :mixloop @14:47:06.47 PRV.dial :ab1 @14:47:07.23 PS.reconfigure :slow @14:47:07.27 PS.seek 0.15ml @14:47:11.05 delay 2 seconds @14:47:13.07 CBV.dial PRV @14:47:13.09
PRV.dial CBV @14:47:13.66 PS.seek 0.35ml @14:47:18.18 delay 2 seconds @14:47:20.19 CBV.dial between PRV and PSV @14:47:21.00 PRV.dial :air @14:47:21.47 PS.seek 0.9ml @14:47:33.21 PRV.dial :waste2 @14:47:33.95 PS.seek 0.5ml @14:47:42.68 addPMLReagent08 @14:47:42.69 PSV.dial :air @14:47:43.15 PS.reconfigure :default @14:47:43.19 PS.seek 2ml @14:47:59.42 PSV.dial :PML @14:47:59.90 PTV.dial :mixing @14:48:00.66 PBV.dial :waste1 @14:48:01.08 PS.seek 1.85ml @14:48:03.39 PBV.dial between PSV and waste1 @14:48:03.78 PS.seek 0ml @14:48:23.71 delay 2 seconds @14:48:25.73 Beginning samplePassC @14:48:25.74 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:48:25.90 Elbow.reconfigure :tight @14:48:25.94 Elbow.seek FlushPuck::Garage @14:48:27.32 Elbow.reconfigure :loose @14:48:27.41 Forearm.seek :garage @14:48:28.34 Hand.close @14:48:29.07 Forearm.seek :retract @14:48:30.05 Elbow.reconfigure :tight @14:48:30.09 Elbow.seek CC @14:48:33.19 Elbow.reconfigure :loose @14:48:33.28 Forearm.reconfigure :tight @14:48:33.32 Forearm.seek CC @14:48:34.54 CC.seek :closed @14:48:39.08 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.36 @14:48:39.14 Hand.open @14:48:39.76 Forearm.reconfigure :loose @14:48:39.80 Forearm.seek :retract @14:48:40.75 PSV.dial PSR @14:48:41.85 Pulling 1.5ml of PSR.flush @14:48:41.86 PSR.select :flush @14:48:41.98 PS.seek 1.5ml @14:48:58.15 delay 5 seconds @14:49:03.17 PSV.dial :waste1 @14:49:03.63 PS.seek 0ml @14:49:19.74 PSV.dial PSR @14:49:20.22 PSR.select :air @14:49:20.33 PS.seek 2.5ml @14:49:46.77 PRV.dial CBV,avoiding: :ab2 @14:49:46.79
CBV.dial PRV @14:49:48.46 PSV.dial :mixloop @14:49:49.50 CTV.dial :air,avoiding: :intake @14:49:49.52 PTV.dial :PRVmixing @14:49:50.28 PS.seek 0ml @14:50:16.64 PSV.dial :air @14:50:17.23 PS.seek 2.5ml @14:50:43.61 PSV.dial :mixloop @14:50:44.05 PS.seek 0ml @14:51:10.45 PSV.dial :air @14:51:11.06 PS.seek 2.5ml @14:51:37.58 PSV.dial :mixloop @14:51:38.02 PS.seek 0ml @14:52:04.36 Garaging FlushPuck in Collection Clamp @14:52:04.43 Elbow.reconfigure :tight @14:52:04.47 Elbow.seek CC @14:52:04.94 Elbow.reconfigure :loose @14:52:05.03 Forearm.seek CC @14:52:06.01 Hand.close @14:52:06.58 CC.seek :open @14:52:11.21 Forearm.seek :retract @14:52:12.18 Elbow.reconfigure :tight @14:52:12.23 Elbow.seek :garage @14:52:15.72 Elbow.reconfigure :loose @14:52:15.81 Forearm.reconfigure :tight @14:52:15.85 Forearm.seek :garage @14:52:16.84 Hand.open @14:52:17.51 Forearm.reconfigure :loose @14:52:17.55 Forearm.seek :retract @14:52:18.56 delayUntil "14:56:25.71" @14:52:18.59 Whole Cell Archive sampling 1000ml @14:52:18.60 Loading wcr puck from tube 3 into Collection Clamp @14:52:19.06 Elbow.reconfigure :tight @14:52:19.10 Elbow.seek 3 @14:52:21.11 Forearm.reconfigure :tight @14:52:21.16 Forearm.seek 3 + 450 counts @14:52:22.19 SE.reconfigure :pick @14:52:22.25 SE.seek :beamBreak @14:52:24.95 SE.configure :default @14:52:25.10 Elbow.seek 3 @14:52:25.27 Forearm.seek 3 + 300 counts @14:52:25.54 Forearm.reconfigure :loose @14:52:25.59 Hand.close @14:52:26.21 SE.seek :down @14:52:26.40 Forearm.seek :retract @14:52:27.38 Elbow.seek CC @14:52:28.63 SE.stop @14:52:29.07 Elbow.reconfigure :loose @14:52:29.16 Forearm.reconfigure :tight @14:52:29.21 Forearm.seek CC @14:52:30.41 CC.seek :closed @14:52:34.92 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.15 @14:52:34.93 Hand.open @14:52:35.63 Forearm.reconfigure :loose @14:52:35.68 Forearm.seek :retract @14:52:36.67 collectSample 1000ml w/bubble point of 35psi @14:52:36.69 Priming 7ml Intake.select :close @14:52:36.70 Exhaust.select :close @14:52:36.71 SS.hold @14:52:36.90 Calibrating pressure sensors @14:52:36.92 CTV.dial :air @14:52:36.93 SSV.dial :spit,avoiding: :exhaust @14:52:36.95 SS.reconfigure :fast @14:52:36.99 SS.seek 5ml @14:52:39.80 SSV.dial CBV @14:52:39.83 CBV.dial SSV @14:52:41.18 delay 3.8 seconds @14:52:45.10 SSV.dial :spit @14:52:46.42 SS.seek 0ml @14:52:49.18 SSV.dial CBV @14:52:50.51 CTV.dial :intake @14:52:51.26 SSV.dial CBV @14:52:51.29 CBV.dial SSV @14:52:51.30 Allowing 40s for 7.0ml of remaining 7.0ml @14:52:51.31 Intake.select :open @14:52:53.47 Intake @15.4psia, vacuum from 9.9 to 12.9psi @14:52:53.49 SS.reconfigure :inhaling 2.20ml/s @14:52:53.53 SS.seek 7ml @14:52:59.16 Stroke completed -- waiting for pressure to equalize ... @14:52:59.17 SS.reconfigure :exhale @14:52:59.21 SS.hold @14:53:10.83 Intake.select :close @14:53:10.97 SSV.dial :exhaust @14:53:11.74 Exhaust.select :open @14:53:11.85 SS.seek 0ml @14:53:15.77 Exhaust.select :close @14:53:15.88 Sampling 1000.0ml @14:53:15.89 Intake.select :close @14:53:15.90 Exhaust.select :close @14:53:15.91 SS.reconfigure :default @14:53:15.95 SS.hold @14:53:16.14 CTV.dial :intake @14:53:16.15 SSV.dial CBV @14:53:16.18 CBV.dial SSV @14:53:16.94 Allowing 130s for 25.0ml of remaining 1000.0ml @14:53:16.96 Intake.select :open @14:53:19.11 Intake @16.0psia, vacuum from 10.0 to 13.0psi @14:53:19.13 SS.reconfigure :inhaling 2.20ml/s @14:53:19.17 SS.seek 25ml @14:53:25.06 Vacuum too high -- waiting up to 40 seconds @14:53:25.07 SS.reconfigure :exhale @14:53:25.11 SS.hold @14:53:27.91 Intake @14.3psia, vacuum from 8.8 to 11.8psi @14:53:27.93 SS.reconfigure :inhaling 1.98ml/s @14:53:27.98 SS.seek 25ml @14:53:33.62 Vacuum too high -- waiting up to 40 seconds @14:53:33.63 SS.reconfigure :exhale @14:53:33.67 SS.hold @14:53:39.55 Intake @14.2psia, vacuum from 8.7 to 11.7psi @14:53:39.57 SS.reconfigure :inhaling 1.79ml/s @14:53:39.62 SS.seek 25ml @14:53:45.85 Stroke completed -- waiting for pressure to equalize ... @14:53:45.86 SS.reconfigure :exhale @14:53:45.90 SS.hold @14:53:53.31 Intake.select :close @14:53:53.45 SSV.dial :exhaust @14:53:54.21 Exhaust.select :open @14:53:54.32 SS.seek 0ml @14:54:03.02 Exhaust.select :close @14:54:03.13 SSV.dial CBV @14:54:03.16 CBV.dial SSV @14:54:03.91 Allowing 130s for 25.0ml of remaining 975.0ml @14:54:03.92 Intake.select :open @14:54:06.08 Intake @15.7psia, vacuum from 10.0 to 13.0psi @14:54:06.10 SS.reconfigure :inhaling 1.79ml/s @14:54:06.14 SS.seek 25ml @14:54:15.24 Vacuum too high -- waiting up to 40 seconds @14:54:15.26 SS.reconfigure :exhale @14:54:15.30 SS.hold @14:54:17.15 Intake @13.2psia, vacuum from 7.7 to 10.7psi @14:54:17.17 SS.reconfigure :inhaling 1.61ml/s @14:54:17.21 SS.seek 25ml @14:54:24.37 Vacuum too high -- waiting up to 40 seconds @14:54:24.38 SS.reconfigure :exhale @14:54:24.42 SS.hold @14:54:31.40 Intake @14.1psia, vacuum from 8.6 to 11.6psi @14:54:31.42 SS.reconfigure :inhaling 1.45ml/s @14:54:31.46 SS.seek 25ml @14:54:34.65 Stroke completed -- waiting for pressure to equalize ... @14:54:34.66 SS.reconfigure :exhale @14:54:34.70 SS.hold @14:54:38.14 Intake.select :close @14:54:38.29 SSV.dial :exhaust @14:54:39.07 Exhaust.select :open @14:54:39.18 SS.seek 0ml @14:54:47.88 Exhaust.select :close @14:54:47.98 SSV.dial CBV @14:54:48.00 CBV.dial SSV @14:54:48.77 Allowing 130s for 25.0ml of remaining 950.0ml @14:54:48.79 Intake.select :open @14:54:50.94 Intake @15.6psia, vacuum from 10.0 to 13.0psi @14:54:50.96 SS.reconfigure :inhaling 1.45ml/s @14:54:51.00 SS.seek 25ml @14:55:03.62 Vacuum too high -- waiting up to 40 seconds @14:55:03.63 SS.reconfigure :exhale @14:55:03.67 SS.hold @14:55:06.28 Intake @13.3psia, vacuum from 7.8 to 10.8psi @14:55:06.30 SS.reconfigure :inhaling 1.30ml/s @14:55:06.34 SS.seek 25ml @14:55:15.39 Stroke completed -- waiting for pressure to equalize ... @14:55:15.40 SS.reconfigure :exhale @14:55:15.45 SS.hold @14:55:24.41 Intake.select :close @14:55:24.55 SSV.dial :exhaust @14:55:25.31 Exhaust.select :open @14:55:25.43 SS.seek 0ml @14:55:34.19 Exhaust.select :close @14:55:34.30 SSV.dial CBV @14:55:34.33 CBV.dial SSV @14:55:35.08 Allowing 130s for 25.0ml of remaining 925.0ml @14:55:35.09 Intake.select :open @14:55:37.25 Intake @15.6psia, vacuum from 10.0 to 13.0psi @14:55:37.27 SS.reconfigure :inhaling 1.30ml/s @14:55:37.31 SS.seek 25ml @14:55:50.74 Vacuum too high -- waiting up to 40 seconds @14:55:50.75 SS.reconfigure :exhale @14:55:50.80 SS.hold @14:55:53.37 Intake @13.5psia, vacuum from 8.0 to 11.0psi @14:55:53.39 SS.reconfigure :inhaling 1.17ml/s @14:55:53.43 SS.seek 25ml @14:56:03.44 Stroke completed -- waiting for pressure to equalize ... @14:56:03.45 SS.reconfigure :exhale @14:56:03.49 SS.hold @14:56:12.00 Intake.select :close @14:56:12.14 SSV.dial :exhaust @14:56:12.91 Exhaust.select :open @14:56:13.02 SS.seek 0ml @14:56:21.98 Exhaust.select :close @14:56:22.09 SSV.dial CBV @14:56:22.11 CBV.dial SSV @14:56:22.89 Allowing 130s for 25.0ml of remaining 900.0ml @14:56:22.91 Intake.select :open @14:56:25.06 Intake @15.5psia, vacuum from 10.0 to 13.0psi @14:56:25.08 SS.reconfigure :inhaling 1.17ml/s @14:56:25.12 SS.seek 25ml @14:56:25.72 recoverPRVreagent08 @14:56:25.73 PTV.dial :mixing @14:56:26.50 PBV.dial :waste1 @14:56:26.87 delay 30 seconds @14:56:41.62 Vacuum too high -- waiting up to 40 seconds @14:56:41.63 SS.reconfigure :exhale @14:56:41.67 SS.hold @14:56:44.43 Intake @13.5psia, vacuum from 8.0 to 11.0psi @14:56:44.45 SS.reconfigure :inhaling 1.06ml/s @14:56:44.49 SS.seek 25ml @14:56:52.47 Stroke completed -- waiting for pressure to equalize ... @14:56:52.48 SS.reconfigure :exhale @14:56:52.52 SS.hold @14:56:56.89 PBV.dial between PSV and waste1 @14:56:57.26 PSV.dial :air @14:56:57.74 PS.reconfigure :fast @14:56:57.79 PS.seek 2.5ml @14:57:00.97 Intake.select :close @14:57:01.12 SSV.dial :exhaust @14:57:01.89 Exhaust.select :open @14:57:02.00 SS.seek 0ml @14:57:10.76 Exhaust.select :close @14:57:10.87 SSV.dial CBV @14:57:10.90 CBV.dial SSV @14:57:11.66 Allowing 130s for 25.0ml of remaining 875.0ml @14:57:11.67 Intake.select :open @14:57:13.83 Intake @15.5psia, vacuum from 10.0 to 13.0psi @14:57:13.84 SS.reconfigure :inhaling 1.06ml/s @14:57:13.89 SS.seek 25ml @14:57:21.60 PSV.dial :mixloop @14:57:22.09 delay 2 seconds @14:57:24.11 PSV.dial :air @14:57:24.60 delay 2 seconds @14:57:26.61 PSV.dial :mixloop @14:57:27.09 delay 2 seconds @14:57:29.11 PSV.dial :air @14:57:29.58 PS.seek 0ml @14:57:34.56 Vacuum too high -- waiting up to 40 seconds @14:57:34.57 SS.reconfigure :exhale @14:57:34.62 SS.hold @14:57:39.49 Intake @14.1psia, vacuum from 8.6 to 11.6psi @14:57:39.51 SS.reconfigure :inhaling 0.95ml/s @14:57:39.55 SS.seek 25ml @14:57:46.06 Stroke completed -- waiting for pressure to equalize ... @14:57:46.07 SS.reconfigure :exhale @14:57:46.11 SS.hold @14:57:52.92 washCycleDA #1 of 2 @14:57:52.93 PSR.select :air @14:57:52.94 PSV.dial PSR @14:57:53.14 Intake.select :close @14:57:53.28 SSV.dial :exhaust @14:57:54.05 Exhaust.select :open @14:57:54.16 SS.seek 0ml @14:57:54.33 Pulling 0.5ml of PSR.wash @14:57:54.34 PSR.select :wash @14:57:54.45 PS.reconfigure :default @14:57:54.50 PS.seek 0.5ml @14:58:00.48 delay 5 seconds @14:58:02.83 Exhaust.select :close @14:58:02.94 SSV.dial CBV @14:58:02.97 CBV.dial SSV @14:58:03.72 Allowing 130s for 25.0ml of remaining 850.0ml @14:58:03.73 Intake.select :open @14:58:05.50 PSR.select :air @14:58:05.61 PS.seek 2ml @14:58:05.89 Intake @15.7psia, vacuum from 10.0 to 13.0psi @14:58:05.91 SS.reconfigure :inhaling 0.95ml/s @14:58:05.95 SS.seek 25ml @14:58:21.76 PTV.dial :puck @14:58:22.51 PSV.dial PTV @14:58:23.28 PBV.dial :waste1 @14:58:23.70 PS.seek 1.5ml @14:58:26.62 Vacuum too high -- waiting up to 40 seconds @14:58:26.63 SS.reconfigure :exhale @14:58:26.67 SS.hold @14:58:29.70 delay 3 seconds @14:58:30.87 Intake @14.2psia, vacuum from 8.7 to 11.7psi @14:58:30.88 SS.reconfigure :inhaling 0.87ml/s @14:58:30.93 SS.seek 25ml @14:58:32.71 PBV.dial between PSV and waste1 @14:58:33.10 PS.seek 0ml @14:58:40.18 Stroke completed -- waiting for pressure to equalize ... @14:58:40.20 SS.reconfigure :exhale @14:58:40.24 SS.hold @14:58:48.81 Intake.select :close @14:58:48.95 SSV.dial :exhaust @14:58:49.17 delay 2:00 @14:58:49.73 Exhaust.select :open @14:58:49.84 SS.seek 0ml @14:58:58.65 Exhaust.select :close @14:58:58.76 SSV.dial CBV @14:58:58.78 CBV.dial SSV @14:58:59.56 Allowing 130s for 25.0ml of remaining 825.0ml @14:58:59.58 Intake.select :open @14:59:01.73 Intake @15.6psia, vacuum from 10.0 to 13.0psi @14:59:01.75 SS.reconfigure :inhaling 0.87ml/s @14:59:01.79 SS.seek 25ml @14:59:24.21 Vacuum too high -- waiting up to 40 seconds @14:59:24.22 SS.reconfigure :exhale @14:59:24.26 SS.hold @14:59:29.59 Intake @14.3psia, vacuum from 8.8 to 11.8psi @14:59:29.60 SS.reconfigure :inhaling 0.78ml/s @14:59:29.65 SS.seek 25ml @14:59:40.00 Stroke completed -- waiting for pressure to equalize ... @14:59:40.01 SS.reconfigure :exhale @14:59:40.05 SS.hold @14:59:53.02 Intake.select :close @14:59:53.16 SSV.dial :exhaust @14:59:53.94 Exhaust.select :open @14:59:54.05 SS.seek 0ml @15:00:02.84 Exhaust.select :close @15:00:02.95 SSV.dial CBV @15:00:02.97 CBV.dial SSV @15:00:03.75 Allowing 130s for 25.0ml of remaining 800.0ml @15:00:03.76 Intake.select :open @15:00:05.92 Intake @15.5psia, vacuum from 10.0 to 13.0psi @15:00:05.94 SS.reconfigure :inhaling 0.78ml/s @15:00:05.98 SS.seek 25ml @15:00:22.02 Vacuum too high -- waiting up to 40 seconds @15:00:22.03 SS.reconfigure :exhale @15:00:22.08 SS.hold @15:00:30.66 Intake @14.9psia, vacuum from 9.4 to 12.4psi @15:00:30.68 SS.reconfigure :inhaling 0.70ml/s @15:00:30.72 SS.seek 25ml @15:00:43.14 Vacuum too high -- waiting up to 40 seconds @15:00:43.15 SS.reconfigure :exhale @15:00:43.20 SS.hold @15:00:49.19 recoverPSRreagent08 @15:00:49.20 PTV.dial :puck @15:00:49.21 PBV.dial :waste1 @15:00:49.59 delay 30 seconds @15:00:57.61 Intake @15.2psia, vacuum from 9.7 to 12.7psi @15:00:57.63 SS.reconfigure :inhaling 0.63ml/s @15:00:57.67 SS.seek 25ml @15:01:07.01 Stroke completed -- waiting for pressure to equalize ... @15:01:07.02 SS.reconfigure :exhale @15:01:07.07 SS.hold @15:01:19.63 PBV.dial between PSV and waste1 @15:01:20.01 PSR.select :air @15:01:20.03 PSV.dial PSR @15:01:20.82 PS.reconfigure :fast @15:01:20.86 PS.seek 2.5ml @15:01:27.36 Intake.select :close @15:01:28.03 SSV.dial :exhaust @15:01:28.79 Exhaust.select :open @15:01:28.90 SS.seek 0ml @15:01:37.75 Exhaust.select :close @15:01:37.85 SSV.dial CBV @15:01:37.87 CBV.dial SSV @15:01:38.65 Allowing 130s for 25.0ml of remaining 775.0ml @15:01:38.67 Intake.select :open @15:01:40.82 Intake @15.6psia, vacuum from 10.0 to 13.0psi @15:01:40.84 SS.reconfigure :inhaling 0.63ml/s @15:01:40.88 SS.seek 25ml @15:01:44.82 PSV.dial PTV @15:01:45.71 delay 5 seconds @15:01:50.73 PSV.dial PSR @15:01:51.48 delay 5 seconds @15:01:55.39 Vacuum too high -- waiting up to 40 seconds @15:01:55.41 SS.reconfigure :exhale @15:01:55.45 SS.hold @15:01:56.49 PSV.dial PTV @15:01:57.27 delay 5 seconds @15:02:02.29 PSV.dial :air @15:02:03.06 PS.seek 0ml @15:02:06.59 Intake @15.2psia, vacuum from 9.7 to 12.7psi @15:02:06.61 SS.reconfigure :inhaling 0.57ml/s @15:02:06.65 SS.seek 25ml @15:02:19.05 Vacuum too high -- waiting up to 40 seconds @15:02:19.06 SS.reconfigure :exhale @15:02:19.11 SS.hold @15:02:26.26 washCycleDA #2 of 2 @15:02:26.27 PSR.select :air @15:02:26.29 PSV.dial PSR @15:02:27.72 Pulling 0.5ml of PSR.wash @15:02:27.73 PSR.select :wash @15:02:27.84 PS.reconfigure :default @15:02:27.88 PS.seek 0.5ml @15:02:33.79 delay 5 seconds @15:02:37.48 SS.reconfigure :inhaling 0.51ml/s @15:02:37.52 SS.seek 25ml @15:02:38.80 PSR.select :air @15:02:38.92 PS.seek 2ml @15:02:53.27 Vacuum too high -- waiting up to 40 seconds @15:02:53.28 SS.reconfigure :exhale @15:02:53.32 SS.hold @15:02:55.07 PTV.dial :puck @15:02:55.08 PSV.dial PTV @15:02:55.83 PBV.dial :waste1 @15:02:56.34 PS.seek 1.5ml @15:03:02.26 delay 3 seconds @15:03:05.27 PBV.dial between PSV and waste1 @15:03:05.66 PS.seek 0ml @15:03:15.81 Intake @15.3psia, vacuum from 9.8 to 12.8psi @15:03:15.83 SS.reconfigure :inhaling 0.47ml/s @15:03:15.87 SS.seek 25ml @15:03:21.52 Stroke completed -- waiting for pressure to equalize ... @15:03:21.54 SS.reconfigure :exhale @15:03:21.58 SS.hold @15:03:21.84 delay 2:00 @15:03:45.99 Intake.select :close @15:03:46.14 SSV.dial :exhaust @15:03:46.92 Exhaust.select :open @15:03:47.03 SS.seek 0ml @15:03:55.98 Exhaust.select :close @15:03:56.08 SSV.dial CBV @15:03:56.11 CBV.dial SSV @15:03:56.87 Allowing 130s for 25.0ml of remaining 750.0ml @15:03:56.88 Intake.select :open @15:03:59.04 Intake @15.5psia, vacuum from 10.0 to 13.0psi @15:03:59.06 SS.reconfigure :inhaling 0.47ml/s @15:03:59.10 SS.seek 25ml @15:04:19.30 Vacuum too high -- waiting up to 40 seconds @15:04:19.31 SS.reconfigure :exhale @15:04:19.35 SS.hold @15:04:39.61 Intake @15.3psia, vacuum from 9.8 to 12.8psi @15:04:39.63 SS.reconfigure :inhaling 0.43ml/s @15:04:39.67 SS.seek 25ml @15:04:55.27 Vacuum too high -- waiting up to 40 seconds @15:04:55.28 SS.reconfigure :exhale @15:04:55.32 SS.hold @15:05:17.78 SS.reconfigure :inhaling 0.38ml/s @15:05:17.82 SS.seek 25ml @15:05:21.86 recoverPSRreagent08 @15:05:21.87 PTV.dial :puck @15:05:21.89 PBV.dial :waste1 @15:05:22.26 delay 30 seconds @15:05:38.89 Vacuum too high -- waiting up to 40 seconds @15:05:38.90 SS.reconfigure :exhale @15:05:38.94 SS.hold @15:05:52.27 PBV.dial between PSV and waste1 @15:05:52.65 PSR.select :air @15:05:52.66 PSV.dial PSR @15:05:53.45 PS.reconfigure :fast @15:05:53.49 PS.seek 2.5ml @15:06:06.89 SS.reconfigure :default @15:06:06.93 SS.hold @15:06:07.06 Intake.select :close @15:06:07.21 ShallowSampler::Clogged in WCR -- Filtering too Slowly after 272.9ml @15:06:07.22 SSV.dial :exhaust @15:06:07.98 Exhaust.select :open @15:06:08.09 SS.reconfigure :exhale @15:06:08.13 SS.seek 0ml @15:06:16.57 Exhaust.select :close @15:06:16.68 Sampled 272.9ml @15:06:16.70 Drying puck before fixing for 20:00 @15:06:16.71 CBV.dial :waste2b @15:06:17.48 delay 1:00 @15:06:17.51 PSV.dial PTV @15:06:18.40 delay 5 seconds @15:06:23.42 PSV.dial PSR @15:06:24.16 delay 5 seconds @15:06:29.18 PSV.dial PTV @15:06:29.94 delay 5 seconds @15:06:34.96 PSV.dial :air @15:06:35.86 PS.seek 0ml @15:06:59.19 getPMLReagent08 @15:06:59.20 PRV.dial :air @15:06:59.66 delay 5 seconds @15:07:04.68 PTV.dial :PRVmixing @15:07:06.04 delay 5 seconds @15:07:11.05 PRV.dial :ab2 @15:07:11.52 PTV.dial :PRVmixing @15:07:11.53 PSV.dial :mixloop @15:07:11.98 PS.reconfigure :default @15:07:12.02 PS.seek 0.3ml @15:07:15.97 delay 2 seconds @15:07:17.50 CSV.dial :air @15:07:17.96 CS.reconfigure :fast @15:07:18.00 CS.seek 10ml @15:07:18.05 PRV.dial :air @15:07:18.58 PS.seek 0.55ml @15:07:21.97 addPMLReagent08 @15:07:21.98 PSV.dial :air @15:07:22.45 PS.seek 2ml @15:07:38.00 PSV.dial :PML @15:07:38.47 PTV.dial :mixing @15:07:39.24 PBV.dial :waste1 @15:07:39.65 PS.seek 1.85ml @15:07:42.10 PBV.dial between PSV and waste1 @15:07:42.44 Pumping wcr puck from tube 3 up to 35psi... @15:07:42.46 CBV.dial SSV @15:07:42.53 PS.seek 0ml @15:07:43.78 CBV.dial between CSV and waste2b @15:07:44.76 CSV.dial between air and CTV @15:07:45.09 CS.seek 7.393ml @15:07:51.91 CTV.dial CSV,avoiding: :air @15:07:51.93 CSV.dial CTV @15:07:52.76 CS.reconfigure :pressurize @15:07:52.80 CS.seek 0ml @15:08:02.32 delay 2 seconds @15:08:04.33 delay 8:00 @15:08:06.80 CBV.dial :waste2b @15:08:07.14 CS.reconfigure :default @15:08:07.19 CS.seek 0ml @15:08:13.26 delay 1:00 @15:09:13.28 CTV.dial :air,avoiding: :intake @15:09:14.63 Fix #1 of 2 (with RNAlater) for 10:00 @15:09:14.64 CSV.dial CSR @15:09:15.13 Pulling 2ml of CSR.RNAlater @15:09:15.14 CSR.select :RNAlater @15:09:15.25 CS.seek 2ml @15:09:21.41 delay 5 seconds @15:09:26.43 CSR.select :air @15:09:26.53 CS.reconfigure :fast @15:09:26.58 CS.seek 10ml @15:09:46.22 Pumping wcr puck from tube 3 up to 35psi... @15:09:46.23 CBV.dial SSV @15:09:47.53 CBV.dial between CSV and waste2b @15:09:48.50 CSV.dial between air and CTV @15:09:49.10 CS.seek 7.251ml @15:09:56.13 CTV.dial CSV,avoiding: :air @15:09:56.15 CSV.dial CTV @15:09:57.55 CS.reconfigure :pressurize @15:09:57.60 CS.seek 0ml @15:10:15.80 delay 10:00 @15:16:04.36 recoverPRVreagent08 @15:16:04.37 PTV.dial :mixing @15:16:04.38 PBV.dial :waste1 @15:16:04.76 delay 30 seconds @15:16:34.79 PBV.dial between PSV and waste1 @15:16:35.15 PSV.dial :air @15:16:35.75 PS.reconfigure :fast @15:16:35.79 PS.seek 2.5ml @15:16:59.68 PSV.dial :mixloop @15:17:00.14 delay 2 seconds @15:17:02.15 PSV.dial :air @15:17:02.62 delay 2 seconds @15:17:04.64 PSV.dial :mixloop @15:17:05.11 delay 2 seconds @15:17:07.13 PSV.dial :air @15:17:07.60 PS.seek 0ml @15:17:30.90 washCycleDA #1 of 3 @15:17:30.91 PSR.select :air @15:17:30.93 PSV.dial PSR @15:17:32.31 Pulling 0.5ml of PSR.wash @15:17:32.32 PSR.select :wash @15:17:32.43 PS.reconfigure :default @15:17:32.48 PS.seek 0.5ml @15:17:38.40 delay 5 seconds @15:17:43.42 PSR.select :air @15:17:43.53 PS.seek 2ml @15:17:59.71 PTV.dial :puck @15:18:00.46 PSV.dial PTV @15:18:01.21 PBV.dial :waste1 @15:18:01.65 PS.seek 1.5ml @15:18:07.52 delay 3 seconds @15:18:10.54 PBV.dial between PSV and waste1 @15:18:10.92 PS.seek 0ml @15:18:27.12 delay 2:00 @15:20:15.82 CBV.dial :waste2b @15:20:16.15 CS.reconfigure :default @15:20:16.20 CS.seek 0ml @15:20:17.68 delay 1:00 @15:20:27.15 recoverPSRreagent08 @15:20:27.16 PTV.dial :puck @15:20:27.17 PBV.dial :waste1 @15:20:27.54 delay 30 seconds @15:20:57.56 PBV.dial between PSV and waste1 @15:20:57.93 PSR.select :air @15:20:57.95 PSV.dial PSR @15:20:58.73 PS.reconfigure :fast @15:20:58.78 PS.seek 2.5ml @15:21:17.70 CTV.dial :air,avoiding: :intake @15:21:19.03 Fix #2 of 2 (with RNAlater) for 10:00 @15:21:19.04 CSV.dial CSR @15:21:19.53 Pulling 2ml of CSR.RNAlater @15:21:19.54 CSR.select :RNAlater @15:21:19.65 CS.seek 2ml @15:21:22.61 PSV.dial PTV @15:21:23.40 delay 5 seconds @15:21:25.52 delay 5 seconds @15:21:28.42 PSV.dial PSR @15:21:29.18 delay 5 seconds @15:21:30.54 CSR.select :air @15:21:30.65 CS.reconfigure :fast @15:21:30.69 CS.seek 10ml @15:21:34.20 PSV.dial PTV @15:21:34.96 delay 5 seconds @15:21:39.98 PSV.dial :air @15:21:40.75 PS.seek 0ml @15:21:50.37 Pumping wcr puck from tube 3 up to 35psi... @15:21:50.38 CBV.dial SSV @15:21:51.69 CBV.dial between CSV and waste2b @15:21:52.66 CSV.dial between air and CTV @15:21:53.73 CS.seek 7.251ml @15:22:00.78 CTV.dial CSV,avoiding: :air @15:22:00.80 CSV.dial CTV @15:22:02.21 CS.reconfigure :pressurize @15:22:02.26 CS.seek 0ml @15:22:04.05 washCycleDA #2 of 3 @15:22:04.06 PSR.select :air @15:22:04.08 PSV.dial PSR @15:22:05.47 Pulling 0.5ml of PSR.wash @15:22:05.48 PSR.select :wash @15:22:05.59 PS.reconfigure :default @15:22:05.63 PS.seek 0.5ml @15:22:11.65 delay 5 seconds @15:22:16.67 PSR.select :air @15:22:16.78 PS.seek 2ml @15:22:18.20 delay 10:00 @15:22:32.96 PTV.dial :puck @15:22:32.97 PSV.dial PTV @15:22:33.72 PBV.dial :waste1 @15:22:34.16 PS.seek 1.5ml @15:22:40.15 delay 3 seconds @15:22:43.16 PBV.dial between PSV and waste1 @15:22:43.55 PS.seek 0ml @15:22:59.68 delay 2:00 @15:24:59.70 recoverPSRreagent08 @15:24:59.71 PTV.dial :puck @15:24:59.72 PBV.dial :waste1 @15:25:00.08 delay 30 seconds @15:25:30.11 PBV.dial between PSV and waste1 @15:25:30.47 PSR.select :air @15:25:30.49 PSV.dial PSR @15:25:31.27 PS.reconfigure :fast @15:25:31.32 PS.seek 2.5ml @15:25:55.20 PSV.dial PTV @15:25:55.99 delay 5 seconds @15:26:01.01 PSV.dial PSR @15:26:01.77 delay 5 seconds @15:26:06.79 PSV.dial PTV @15:26:07.55 delay 5 seconds @15:26:12.57 PSV.dial :air @15:26:13.34 PS.seek 0ml @15:26:36.49 washCycleDA #3 of 3 @15:26:36.50 PSR.select :air @15:26:36.51 PSV.dial PSR @15:26:37.90 Pulling 0.5ml of PSR.wash @15:26:37.91 PSR.select :wash @15:26:38.02 PS.reconfigure :default @15:26:38.06 PS.seek 0.5ml @15:26:44.05 delay 5 seconds @15:26:49.07 PSR.select :air @15:26:49.18 PS.seek 2ml @15:27:05.36 PTV.dial :puck @15:27:05.37 PSV.dial PTV @15:27:06.11 PBV.dial :waste1 @15:27:06.54 PS.seek 1.5ml @15:27:12.52 delay 3 seconds @15:27:15.53 PBV.dial between PSV and waste1 @15:27:15.91 PS.seek 0ml @15:27:32.02 delay 2:00 @15:29:32.04 recoverPSRreagent08 @15:29:32.05 PTV.dial :puck @15:29:32.06 PBV.dial :waste1 @15:29:32.42 delay 30 seconds @15:30:02.45 PBV.dial between PSV and waste1 @15:30:02.81 PSR.select :air @15:30:02.83 PSV.dial PSR @15:30:03.60 PS.reconfigure :fast @15:30:03.64 PS.seek 2.5ml @15:30:27.48 PSV.dial PTV @15:30:28.25 delay 5 seconds @15:30:33.27 PSV.dial PSR @15:30:34.03 delay 5 seconds @15:30:39.05 PSV.dial PTV @15:30:39.81 delay 5 seconds @15:30:44.83 PSV.dial :air @15:30:45.60 PS.seek 0ml @15:31:09.07 Gate.power :camera,:ON @15:31:09.09 Mixing DA Substrates @15:31:09.10 PSR.select :air @15:31:09.11 PSV.dial PSR @15:31:10.66 Pulling 0.25ml of PSR.sub1 @15:31:10.68 PSR.select :sub1 @15:31:10.91 PS.reconfigure :default @15:31:10.99 PS.seek 0.25ml @15:31:14.43 delay 5 seconds @15:31:19.49 Pulling 0.25ml of PSR.sub2 @15:31:19.50 PSR.select :sub2 @15:31:19.63 PS.seek 0.5ml @15:31:23.05 delay 5 seconds @15:31:28.06 PSR.select :air @15:31:28.17 PS.seek 2ml @15:31:44.35 PTV.dial :puck @15:31:44.37 PSV.dial PTV @15:31:45.23 PBV.dial :waste1 @15:31:45.66 PS.seek 1.5ml @15:31:51.67 delay 3 seconds @15:31:54.69 PBV.dial between PSV and waste1 @15:31:55.07 PS.seek 0ml @15:32:11.29 delay 10 seconds @15:32:18.22 CBV.dial :waste2b @15:32:18.57 CS.reconfigure :default @15:32:18.61 CS.seek 0ml @15:32:21.31 recoverPSRreagent08 @15:32:21.32 PTV.dial :puck @15:32:21.33 PBV.dial :waste1 @15:32:22.20 delay 30 seconds @15:32:22.39 delay 1:00 @15:32:52.23 PBV.dial between PSV and waste1 @15:32:52.59 PSR.select :air @15:32:52.61 PSV.dial PSR @15:32:53.38 PS.reconfigure :fast @15:32:53.42 PS.seek 2.5ml @15:33:17.38 PSV.dial PTV @15:33:18.17 delay 5 seconds @15:33:22.42 CTV.dial :air,avoiding: :intake @15:33:23.18 PSV.dial PSR @15:33:23.95 delay 5 seconds @15:33:28.97 PSV.dial PTV @15:33:29.73 delay 5 seconds @15:33:34.75 PSV.dial :air @15:33:35.66 PS.seek 0ml @15:33:59.39 Additional air clear @15:33:59.40 PSR.select :air @15:33:59.41 PSV.dial PSR @15:34:00.83 Pulling 2ml of PSR.air @15:34:00.84 PSR.select :air @15:34:00.86 PS.reconfigure :default @15:34:00.90 PS.seek 2ml @15:34:22.20 delay 5 seconds @15:34:27.22 PTV.dial :puck @15:34:27.23 PSV.dial PTV @15:34:28.01 PBV.dial :waste1 @15:34:28.43 PS.seek 1.5ml @15:34:34.35 delay 3 seconds @15:34:37.37 PBV.dial between PSV and waste1 @15:34:37.75 delay 1 second @15:34:38.76 PSV.dial :waste1 @15:34:39.86 PS.seek 0ml @15:34:56.21 Processing Puck Heater at 28C before imaging @15:34:56.22 PH.coast @15:34:56.35 Imaging da puck in Processing Clamp while storing it to tube 2 @15:34:56.50 SC.reconfigure :tight @15:34:56.55 SC.seek 2 @15:35:00.27 SC.configure :limp @15:35:00.46 Elbow.reconfigure :tight @15:35:00.51 Elbow.seek 2 @15:35:02.31 Forearm.reconfigure :tight @15:35:02.36 Forearm.seek 2 + 450 counts @15:35:03.40 SE.reconfigure :pick @15:35:03.45 SE.seek :beamBreak @15:35:05.09 SE.configure :default @15:35:05.24 Elbow.reconfigure :limp @15:35:05.29 Forearm.reconfigure :loose @15:35:05.33 Forearm.seek 2 @15:35:05.58 Elbow.hold @15:35:05.64 Elbow.reconfigure :tight @15:35:05.72 SE.seek 24.1 Pucks below Top Plate @15:35:06.25 Forearm.force -20 @15:35:06.29 Forearm.seek 2 + 284 counts @15:35:06.56 Forearm.reconfigure :tight @15:35:06.61 Forearm.seek 2 + 450 counts @15:35:06.80 SE.seek 25.1 Pucks below Top Plate @15:35:07.44 Forearm.reconfigure :loose @15:35:07.49 Forearm.seek :retract @15:35:08.42 Elbow.seek PC @15:35:11.11 Elbow.reconfigure :loose @15:35:11.20 Forearm.seek PC @15:35:12.18 Hand.close @15:35:12.80 PC.seek :open @15:35:17.54 Forearm.seek :retract @15:35:18.49 Elbow.reconfigure :tight @15:35:18.53 Elbow.seek 2 @15:35:21.18 Forearm.reconfigure :tight @15:35:21.23 Forearm.seek 2 + 450 counts @15:35:22.15 Hand.open @15:35:22.85 SE.seek 24.75 Pucks below Top Plate @15:35:23.11 SE.reconfigure :seal @15:35:23.15 SE.seek 23.79 Pucks below Top Plate @15:35:23.67 Lights, camera, ACTION! @15:35:23.70 PuckCamera: snap -bin=2,2 -auto /var/log/esp/da23feb0715h425ml.tif @15:35:43.20 Waiting >20 seconds for da to relinquish FlushPuck @15:35:44.43 SX HX9/16 (USB:15): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 17.457 seconds /var/log/esp/da23feb0715h425ml.tif: TIFF Upload Complete @15:35:44.45 PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da23feb0715h425ml15s.tif @15:36:00.07 SX HX9/16 (USB:15): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15 seconds /var/log/esp/midres/da23feb0715h425ml15s.tif: TIFF Upload Complete @15:36:00.09 PuckCamera: snap -bin=2,2 20.0 /var/log/esp/midres/da23feb0715h425ml20s.tif @15:36:20.75 SX HX9/16 (USB:15): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 20 seconds /var/log/esp/midres/da23feb0715h425ml20s.tif: TIFF Upload Complete @15:36:20.76 Gate.power :camera,:OFF @15:36:20.78 DAfans.email "17.457 sec auto-exposure",Subject: "da23feb0715h425ml images!" @15:36:21.02 Elbow.seek 2 @15:36:21.81 Forearm.seek 2 + 450 counts @15:36:21.88 SE.reconfigure :gently @15:36:21.93 SE.seek 24.5 Pucks below Top Plate @15:36:22.59 Elbow.reconfigure :limp @15:36:22.66 Forearm.reconfigure :loose @15:36:22.70 Forearm.seek 2 @15:36:22.96 Elbow.hold @15:36:23.04 Elbow.reconfigure :tight @15:36:23.09 SE.reconfigure :default @15:36:23.14 SE.seek 24.69 Pucks below Top Plate @15:36:23.55 Forearm.force -20 @15:36:23.59 Forearm.seek 2 + 274 counts @15:36:23.89 Forearm.reconfigure :tight @15:36:23.93 Forearm.seek 2 + 450 counts @15:36:24.29 SE.seek :down @15:36:25.45 Forearm.reconfigure :loose @15:36:25.47 SE.stop @15:36:25.56 Forearm.seek :retract @15:36:26.70 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @15:36:27.17 Elbow.seek FlushPuck::Garage @15:36:29.28 Elbow.reconfigure :loose @15:36:29.46 Forearm.seek :garage @15:36:31.01 Hand.close @15:36:31.93 Forearm.seek :retract @15:36:32.99 Elbow.reconfigure :tight @15:36:33.07 Elbow.seek PC @15:36:34.45 Elbow.reconfigure :loose @15:36:34.65 Forearm.reconfigure :tight @15:36:34.77 Forearm.seek PC @15:36:36.31 PC.seek :closed @15:36:41.05 Processing Clamp onSpring/offSpring for 16/26 samples, on/off torque 1.59 @15:36:41.20 Hand.open @15:36:41.93 Forearm.reconfigure :loose @15:36:42.03 Forearm.seek :retract @15:36:43.02 PSR.select :air @15:36:43.08 Storing wcr puck in Collection Clamp to tube 2 @15:36:43.11 PSV.dial PSR @15:36:44.00 Pulling 0.5ml of PSR.flush @15:36:44.06 PSR.select :flush @15:36:44.26 Elbow.reconfigure :tight @15:36:44.36 Elbow.seek 2 @15:36:44.54 PS.seek 0.5ml @15:36:47.23 Forearm.reconfigure :tight @15:36:47.32 Forearm.seek 2 + 450 counts @15:36:48.61 SE.reconfigure :pick @15:36:48.72 SE.seek :beamBreak @15:36:50.20 SE.configure :default @15:36:50.54 delay 5 seconds @15:36:50.58 Elbow.reconfigure :limp @15:36:50.67 Forearm.reconfigure :loose @15:36:50.76 Forearm.seek 2 @15:36:52.26 Elbow.hold @15:36:52.42 Elbow.reconfigure :tight @15:36:52.63 SE.seek 25.1 Pucks below Top Plate @15:36:53.25 Forearm.force -20 @15:36:53.33 Forearm.seek 2 + 265 counts @15:36:53.78 Forearm.reconfigure :tight @15:36:53.87 Forearm.seek 2 + 450 counts @15:36:54.10 SE.seek 26.1 Pucks below Top Plate @15:36:54.91 Forearm.reconfigure :loose @15:36:55.00 Forearm.seek :retract @15:36:55.58 PSR.select :air @15:36:55.74 PS.seek 2.5ml @15:36:55.98 Elbow.seek CC @15:36:57.49 upsync @15:36:57.62 Elbow.reconfigure :loose @15:36:57.71 Forearm.seek CC @15:36:58.76 Hand.close @15:36:59.42 CC.seek :open @15:37:04.13 Forearm.seek :retract @15:37:05.11 Elbow.reconfigure :tight @15:37:05.16 Elbow.seek 2 @15:37:06.93 Elbow.reconfigure :limp @15:37:06.98 Forearm.seek 2 @15:37:07.98 Elbow.hold @15:37:08.05 Elbow.reconfigure :tight @15:37:08.10 Hand.open @15:37:08.78 Forearm.force -20 @15:37:08.82 Forearm.seek 2 + 310 counts @15:37:09.23 Forearm.reconfigure :tight @15:37:09.27 Forearm.seek 2 + 450 counts @15:37:09.52 SE.seek :down @15:37:10.47 SE.stop @15:37:10.65 Forearm.reconfigure :loose @15:37:10.69 Forearm.seek :retract @15:37:11.60 Starting finalPurge @15:37:16.98 delay 1 second @15:37:17.99 PRV.dial :waste2,avoiding: :ab2 @15:37:19.88 PTV.dial :PRVmixing @15:37:21.23 PSV.dial :mixloop @15:37:22.28 PS.seek :empty @15:37:31.01
Waiting >20 seconds for psFlush to relinquish FlushPuck @15:37:48.69 PSR.select :air @15:37:48.71 PSV.dial PSR @15:37:49.81 Pulling 0.5ml of PSR.flush @15:37:49.82 PSR.select :flush @15:37:49.93 PS.seek 0.5ml @15:37:55.95 delay 5 seconds @15:38:00.96 PSR.select :air @15:38:01.08 PS.seek 2.5ml @15:38:22.35 delay 1 second @15:38:23.37 PBV.dial :waste1 @15:38:23.72 PTV.dial :mixing @15:38:24.47 PSV.dial :mixloop @15:38:25.54 PS.seek :empty @15:38:51.91 PSV.dial PSR @15:38:52.97 PSR.select :flush @15:38:53.09 PS.seek 1ml @15:39:04.16 PSR.select :air @15:39:04.27 PS.seek 2.5ml @15:39:20.38 PSV.dial PTV @15:39:21.27 PTV.dial :puck @15:39:22.52 PBV.dial :waste1 @15:39:22.54 PS.seek 0ml @15:39:48.94 PSV.dial PSR @15:39:49.69 PSR.select :flush @15:39:49.81 PS.seek 0.5ml @15:39:55.81 PSR.select :air @15:39:55.93 PS.seek 2.5ml @15:40:17.19 PSV.dial PTV @15:40:17.96 PBV.dial PSV @15:40:18.54 PS.seek 0ml @15:40:45.38 PSV.dial PBV @15:40:45.85 PS.seek 2.5ml @15:41:12.25 PSV.dial :waste1 @15:41:13.01 PS.seek 0ml @15:41:39.34 PSV.dial PSR @15:41:39.81 PSR.select :air @15:41:39.84 PS.seek 2.5ml @15:42:06.22 PSV.dial PTV @15:42:06.97 PBV.dial PSV @15:42:06.99 PS.seek 0ml @15:42:33.31 PSV.dial PBV @15:42:33.79 PS.seek 2.5ml @15:43:00.09 PSV.dial :waste1 @15:43:00.85 PS.seek 0ml @15:43:27.22 PSV.dial PSR @15:43:27.69 PSR.select :flush @15:43:27.80 PS.seek 1ml @15:43:38.88 PSR.select :air @15:43:38.99 PS.seek 2.5ml @15:43:55.15 PSV.dial PTV @15:43:55.91 PTV.dial :puck @15:43:55.92 PBV.dial :waste1 @15:43:56.49 PS.seek 0ml @15:44:22.84 PSV.dial PSR @15:44:23.61 PSR.select :flush @15:44:23.72 PS.seek 0.5ml @15:44:29.65 PSR.select :air @15:44:29.77 PS.seek 2.5ml @15:44:51.00 PSV.dial PTV @15:44:51.76 PBV.dial PSV @15:44:52.33 PS.seek 0ml @15:45:18.72 PSV.dial PBV @15:45:19.19 PS.seek 2.5ml @15:45:45.47 PSV.dial :waste1 @15:45:46.23 PS.seek 0ml @15:46:12.59 PSV.dial PSR @15:46:13.06 PSR.select :air @15:46:13.08 PS.seek 2.5ml @15:46:39.37 PSV.dial PTV @15:46:40.12 PBV.dial PSV @15:46:40.14 PS.seek 0ml @15:47:06.50 PSV.dial PBV @15:47:06.97 PS.seek 2.5ml @15:47:33.31 PSV.dial :waste1 @15:47:34.07 PS.seek 0ml @15:48:00.38
Loading FlushPuck into Collection Clamp from Processing Clamp @15:48:00.54 Elbow.seek PC @15:48:03.14 Elbow.reconfigure :loose @15:48:03.23 Forearm.seek PC @15:48:04.18 Hand.close @15:48:04.79 PC.seek :open @15:48:09.54 Forearm.seek :retract @15:48:10.54 Elbow.reconfigure :tight @15:48:10.58 Elbow.seek CC @15:48:14.37 Elbow.reconfigure :loose @15:48:14.50 Forearm.reconfigure :tight @15:48:14.54 Forearm.seek CC @15:48:15.75 CC.seek :closed @15:48:20.25 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.42 @15:48:20.32 Hand.open @15:48:20.97 Forearm.reconfigure :loose @15:48:21.01 Forearm.seek :retract @15:48:22.01 Flushing the Collection Valve::Manifold @15:48:22.12 CSV.dial CSR @15:48:22.60 Pulling 5ml of CSR.flush @15:48:22.61 CSR.select :flush @15:48:22.72 CS.seek 5ml @15:48:36.50 delay 5 seconds @15:48:41.52 CSR.select :air @15:48:41.80 CS.reconfigure :fast @15:48:41.93 CS.seek 7.501ml @15:48:48.68 CSV.dial :waste @15:48:50.44 CS.reconfigure :default @15:48:50.48 CS.seek 0ml @15:49:10.54 Flushing lower manifold's waste1 port @15:49:10.65 CSV.dial :flush @15:49:11.11 CS.reconfigure :fast @15:49:11.16 CS.seek 5ml @15:49:23.94 delay 5 seconds @15:49:28.95 CSV.dial :air @15:49:29.58 CS.seek 6.999ml @15:49:35.28 CSV.dial CTV @15:49:35.30
CTV.dial CSV @15:49:36.70 CBV.dial :waste1 @15:49:37.46 CS.seek 0ml @15:49:54.06 Flushing lower manifold's waste2 port @15:49:54.18 CSV.dial :flush @15:49:54.91 CS.seek 5ml @15:50:07.72 delay 5 seconds @15:50:12.73 CSV.dial :air @15:50:13.25 CS.seek 7ml @15:50:18.87 CSV.dial CTV @15:50:18.90
CTV.dial CSV @15:50:19.34 CBV.dial :waste2 @15:50:20.41 CS.seek 0ml @15:50:37.25 Flushing lower manifold's waste2b port @15:50:37.36 CSV.dial :flush @15:50:38.10 CS.seek 5ml @15:50:50.84 delay 5 seconds @15:50:55.86 CSV.dial :air @15:50:56.37 CS.seek 7ml @15:51:02.15 CSV.dial CTV @15:51:02.18
CTV.dial CSV @15:51:02.61 CBV.dial :waste2b @15:51:03.66 CS.seek 0ml @15:51:20.59 Disinfecting external Intake with kill @15:51:20.70 CSV.dial CSR @15:51:21.31 Pulling 8ml of CSR.kill @15:51:21.32 CSR.select :kill @15:51:21.44 CS.seek 8ml @15:51:41.12 delay 5 seconds @15:51:46.14 CSR.select :air @15:51:46.25 CS.seek 10ml @15:51:51.78 CSV.dial CBV @15:51:51.80
CBV.dial CSV @15:51:52.87 CTV.dial :intake @15:51:53.65 Intake.select :open @15:51:53.76 CS.seek 2ml @15:52:12.74 Intake.select :close @15:52:12.84 CTV.dial :air @15:52:13.61 CS.reconfigure :default @15:52:13.65 CS.seek 0ml @15:52:19.59 CSV.dial CSR @15:52:20.66 Pulling 5ml of CSR.flush CSR.select :flush @15:52:20.78 CS.seek 5ml @15:52:34.56 delay 5 seconds @15:52:39.58 CSR.select :air @15:52:39.69 CS.seek 10ml @15:52:53.34 CSV.dial CBV @15:52:54.49 CS.seek 0ml @15:53:20.93 Garaging FlushPuck in Collection Clamp @15:53:21.00 Elbow.reconfigure :tight @15:53:21.04 Elbow.seek CC @15:53:21.46 Elbow.reconfigure :loose @15:53:21.55 Forearm.seek CC @15:53:22.49 Hand.close @15:53:23.04 CC.seek :open @15:53:27.66 Forearm.seek :retract @15:53:28.65 Elbow.reconfigure :tight @15:53:28.69 Elbow.seek :garage @15:53:31.71 Elbow.reconfigure :loose @15:53:31.92 Forearm.reconfigure :tight @15:53:31.96 Forearm.seek :garage @15:53:32.93 Hand.open @15:53:33.59 Forearm.reconfigure :loose @15:53:33.63 Forearm.seek :retract @15:53:34.65 Completed [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] begun 13:39:04 @15:53:34.69 Asleep after completing DA_WCR_020723 assays @15:53:34.71 GoodNews.email "{1 => 33,\n 2 => 27,\n 3 => 6,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@15:48:15, 21% flow, 13.1C, 35% humidity, 14.4psia, 13.744V, 0.363A, 0.351A avg, 4.99W\nSampled 272.9ml",Subject: "Asleep after completing DA_WCR_020723 assays" @15:53:41.05 upsync @20:47:43.56UTC07-Feb-23
ESP2 v3.41 I2C Gateway v3.20 for core bus @20:47:46.13 Configuration for short puck jake 1/11/21 by brent@mbari.org /home/esp/esp2/type/shallow/jake/configure.rb @20:47:50.03 Polling Can every 10:00 @20:47:50.21 Cleanly exited at 20:44:39UTC07-Feb-23 @20:47:50.24 Loading /home/esp/esp2/mission/DA_WCR_020723b.rb mission @20:47:50.30 Preparing to sleep until + 00:00:05 @20:47:50.31 GoodNews.email "Starting DA_WCR_020723b w/pucks in tube 3\n{1 => 33,\n 2 => 27,\n 3 => 6,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@20:47:50, 17% flow, 17.5C, 31% humidity, 14.6psia, 13.749V, 0.327A, 0.315A avg, 4.49W",Subject: "DA_WCR_020723b begun" @20:47:50.35
Asleep til + 00:00:05 @20:47:50.39 Continuing at 20:47 @20:47:50.63 Gate.power :core,:OFF @20:47:50.65 Gate.power :raw,:OFF @20:47:50.66 Shutdown @20:47:53.94 GoodNews.email "Then, in tube 3: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2/mission/DA_WCR_020723b.rb:14\n{1 => 33,\n 2 => 27,\n 3 => 6,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@20:47:50, 16% flow, 17.5C, 31% humidity, 14.6psia, 13.754V, 0.327A, 0.315A avg, 4.49W",Subject: "Asleep until 20:47" @20:47:55.28
Powering core bus @20:47:55.71 upsync @20:47:59.03 upsync @20:48:01.12
Gate.power :raw,:ON @20:48:02.04 CS.configure :default @20:48:02.13 PS.configure :default @20:48:02.23 SS.configure :default @20:48:02.32 CC.configure :default @20:48:02.41 PC.configure :default @20:48:02.51 Forearm.configure :loose @20:48:02.60 Elbow.configure :loose @20:48:02.71 SC.configure :loose @20:48:02.80 SE.configure :default @20:48:02.85 CH.coast @20:48:02.90 CH.configure :default @20:48:02.93 PH.coast @20:48:02.98 PH.configure :default @20:48:03.02 All dwarf controllers running firmware version 3.85 @20:48:03.12 Valve.reset @20:48:03.31 Intake.select :close @20:48:03.37 Exhaust.select :close @20:48:03.42 CTV.dial :air @20:48:03.51 PSV.dial :air @20:48:03.63 CSV.dial :waste1 @20:48:03.65
Forearm.reconfigure :loose @20:48:03.74 Forearm.home.seek :retract @20:48:04.13 SSV.dial :spit,avoiding: :exhaust @20:48:04.43 Hand.close @20:48:04.46
Elbow.jog 1500 @20:48:04.57 PS.reconfigure :default @20:48:04.62 PS.home.seek 0ml @20:48:04.95 CS.reconfigure :fast @20:48:04.98 SS.reconfigure :default @20:48:05.09 CS.home.seek 0ml @20:48:05.10 SS.home.seek 0ml @20:48:05.21
Elbow.reconfigure :loose @20:48:05.25 Elbow.home.seek :clear @20:48:08.57 SE.reconfigure :default @20:48:08.61 SE.home.seek :down @20:48:09.21 SC.reconfigure :tight @20:48:09.25 SC.home.seek :initial @20:48:12.86 PS.stop @20:48:21.83
SC.configure :limp @20:48:21.87 Forearm.seek :retract @20:48:21.93 Elbow.seek :hammer @20:48:22.96 Forearm.reconfigure :tight @20:48:23.00 Forearm.seek retracted @20:48:23.21 CC.reconfigure :default @20:48:23.23 PC.reconfigure :default @20:48:23.31
CC.home.seek :open @20:48:23.33 PC.home.seek :open @20:48:26.86
Phase [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] @20:48:26.87 Notice.email "At 20:48:26 began in tube 3: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2/mission/DA_WCR_020723b.rb:14\n{1 => 33,\n 2 => 27,\n 3 => 6,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@20:48:26, 16% flow, 17.5C, 31% humidity, 14.6psia, 13.749V, 0.321A, 0.302A avg, 4.41W",Subject: "Started DA_WCR_020723b phase initialPurge; da [1000, 1000] #da and wsr are run simultanelously" @20:48:26.90
Starting initialPurge @20:48:26.92 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @20:48:26.98 Hand.open @20:48:27.72 Elbow.reconfigure :tight @20:48:27.76 Elbow.seek FlushPuck::Garage @20:48:29.61 Elbow.reconfigure :loose @20:48:29.70 Forearm.reconfigure :loose @20:48:29.74 Forearm.seek :garage @20:48:30.70 Hand.close @20:48:31.47 Forearm.seek :retract @20:48:31.77 upsync @20:48:32.44
Elbow.reconfigure :tight @20:48:32.49 Elbow.seek CC @20:48:35.41 Elbow.reconfigure :loose @20:48:35.50 Forearm.reconfigure :tight @20:48:35.54 Forearm.seek CC @20:48:36.76 CC.seek :closed @20:48:41.29 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.35 @20:48:41.35 Hand.open @20:48:42.03 Forearm.reconfigure :loose @20:48:42.07 Forearm.seek :retract @20:48:43.07 Flushing the Collection Valve::Manifold @20:48:43.19 CSV.dial CSR @20:48:44.51 Pulling 5ml of CSR.flush @20:48:44.52 CSR.select :flush @20:48:44.63 CS.reconfigure :default @20:48:44.67 CS.seek 5ml @20:48:58.47 delay 5 seconds @20:49:03.49 CSR.select :air @20:49:03.64 CS.reconfigure :fast @20:49:03.69 CS.seek 7.5ml @20:49:10.41 CSV.dial :waste @20:49:11.68 CS.reconfigure :default @20:49:11.72 CS.seek 0ml @20:49:31.72 Purging external Intake @20:49:31.84 CSV.dial :flush @20:49:32.29 CS.reconfigure :fast @20:49:32.33 CS.seek 10ml @20:49:56.72 delay 5 seconds @20:50:01.74 CSV.dial CBV @20:50:01.77
CBV.dial CSV @20:50:02.52 CTV.dial :intake @20:50:03.28 Intake.select :open @20:50:03.39 CS.seek 0ml @20:50:26.79 Intake.select :close @20:50:26.89 CTV.dial :air @20:50:27.67 Flushing upper manifold @20:50:27.79 CSV.dial :flush @20:50:28.53 CS.seek 5ml @20:50:41.28 delay 5 seconds @20:50:46.30 CSV.dial :air @20:50:46.79 CS.seek 7ml @20:50:52.25 CSV.dial CBV @20:50:52.27
CBV.dial CSV @20:50:53.30 CTV.dial :air @20:50:53.32 CS.seek 0ml @20:51:09.81 delay 7 seconds @20:51:16.84 CS.seek 3ml @20:51:24.81 CSV.dial :waste @20:51:25.26 CS.seek 0ml @20:51:33.07 CSV.dial :flush @20:51:33.51 CS.seek 3ml @20:51:41.44 delay 5 seconds @20:51:46.45 CSV.dial :air @20:51:47.08 CS.seek 5ml @20:51:52.56 CSV.dial :air @20:51:52.58 CS.seek 0ml @20:52:04.87 delay 7 seconds @20:52:11.90 CS.seek 3ml @20:52:19.81 CSV.dial :waste @20:52:20.52 CS.seek 0ml @20:52:28.19 Garaging FlushPuck in Collection Clamp @20:52:28.25 Elbow.reconfigure :tight @20:52:28.30 Elbow.seek CC @20:52:28.65 Elbow.reconfigure :loose @20:52:28.74 Forearm.seek CC @20:52:29.75 Hand.close @20:52:30.33 CC.seek :open @20:52:34.98 Forearm.seek :retract @20:52:35.96 Elbow.reconfigure :tight @20:52:36.00 Elbow.seek :garage @20:52:38.96 Elbow.reconfigure :loose @20:52:39.05 Forearm.reconfigure :tight @20:52:39.09 Forearm.seek :garage @20:52:40.09 Hand.open @20:52:41.20 Forearm.reconfigure :loose @20:52:41.24 Forearm.seek :retract @20:52:42.21 da sampling at most 1000ml, wcr at most 1000ml @20:52:42.24 Loading sh1 puck from tube 3 into Collection Clamp @20:52:42.25 DAfans.email "Started da in tube 3 at 20:52:42UTC07-Feb-23\n{1 => 33,\n 2 => 27,\n 3 => 6,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@20:48:26, 16% flow, 17.5C, 31% humidity, 14.6psia, 13.749V, 0.321A, 0.302A avg, 4.41W",Subject: "da sampling at most 1000ml, wcr at most 1000ml" @20:52:42.50
SC.reconfigure :tight @20:52:42.55 SC.seek 3 @20:52:46.13 SC.configure :limp @20:52:46.34 Elbow.reconfigure :tight @20:52:46.38 Elbow.seek 3 @20:52:47.30 upsync @20:52:48.28
Forearm.reconfigure :tight @20:52:48.32 Forearm.seek 3 + 450 counts @20:52:49.35 SE.reconfigure :pick @20:52:49.40 SE.seek :beamBreak @20:52:52.27 SE.configure :default @20:52:52.41 Elbow.seek 3 @20:52:52.58 Forearm.seek 3 + 300 counts @20:52:52.75 Forearm.reconfigure :loose @20:52:52.79 Hand.close @20:52:53.47 SE.seek :down @20:52:53.66
Forearm.seek :retract @20:52:54.62 Elbow.seek CC @20:52:55.88 SE.stop @20:52:56.32
Elbow.reconfigure :loose @20:52:56.41 Forearm.reconfigure :tight @20:52:56.46 Forearm.seek CC @20:52:57.76 CC.seek :closed @20:53:02.32 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.20 @20:53:02.33 Hand.open @20:53:03.03 Forearm.reconfigure :loose @20:53:03.07 Forearm.seek :retract @20:53:04.04 collectSample 1000ml w/bubble point of 24psi @20:53:04.05 Priming 7ml @20:53:04.06 Intake.select :close @20:53:04.07 Exhaust.select :close @20:53:04.08 SS.hold @20:53:04.28 Calibrating pressure and temperature sensors @20:53:04.31 CTV.dial :air @20:53:04.32 SSV.dial :spit,avoiding: :exhaust @20:53:04.34 SS.reconfigure :fast @20:53:04.38 SS.seek 5ml @20:53:07.25 SSV.dial CBV @20:53:07.28
CBV.dial SSV @20:53:08.57 delay 3.8 seconds @20:53:12.48 SSV.dial :spit @20:53:13.80 SS.seek 0ml @20:53:16.63 SSV.dial CBV @20:53:17.95 CTV.dial :intake @20:53:18.72 SSV.dial CBV @20:53:18.74
CBV.dial SSV @20:53:18.75 Allowing 40s for 7.0ml of remaining 7.0ml @20:53:18.76 Intake.select :open @20:53:20.92 Intake @15.2psia, vacuum from 9.7 to 12.7psi @20:53:20.94 SS.reconfigure :inhaling 2.20ml/s @20:53:20.98 SS.seek 7ml @20:53:27.77 Stroke completed -- waiting for pressure to equalize ... @20:53:27.78 SS.reconfigure :exhale @20:53:27.82 SS.hold @20:53:30.22 Intake.select :close @20:53:30.36 SSV.dial :exhaust @20:53:31.12 Exhaust.select :open @20:53:31.22 SS.seek 0ml @20:53:35.69 Exhaust.select :close @20:53:35.80 Sampling 1000.0ml @20:53:35.81 Intake.select :close @20:53:35.82 Exhaust.select :close @20:53:35.83 SS.reconfigure :default @20:53:35.87 SS.hold @20:53:36.05 CTV.dial :intake @20:53:36.06 SSV.dial CBV @20:53:36.08
CBV.dial SSV @20:53:36.85 Allowing 130s for 25.0ml of remaining 1000.0ml @20:53:36.86 Intake.select :open @20:53:39.01 Intake @15.3psia, vacuum from 9.8 to 12.8psi @20:53:39.03 SS.reconfigure :inhaling 2.20ml/s @20:53:39.07 SS.seek 25ml @20:53:48.84 Vacuum too high -- waiting up to 40 seconds @20:53:48.85 SS.reconfigure :exhale @20:53:48.89 SS.hold @20:53:49.56 Intake @12.0psia, vacuum from 6.5 to 9.5psi @20:53:49.58 SS.reconfigure :inhaling 1.98ml/s @20:53:49.62 SS.seek 25ml @20:53:54.41 Vacuum too high -- waiting up to 40 seconds @20:53:54.42 SS.reconfigure :exhale @20:53:54.46 SS.hold @20:54:01.21 Intake @13.7psia, vacuum from 8.2 to 11.2psi @20:54:01.23 SS.reconfigure :inhaling 1.79ml/s @20:54:01.27 SS.seek 25ml @20:54:02.52 Stroke completed -- waiting for pressure to equalize ... @20:54:02.53 SS.reconfigure :exhale @20:54:02.57 SS.hold @20:54:03.44 Intake.select :close @20:54:03.58 SSV.dial :exhaust @20:54:04.32 Exhaust.select :open @20:54:04.43 SS.seek 0ml @20:54:13.44 Exhaust.select :close @20:54:13.55 SSV.dial CBV @20:54:13.58
CBV.dial SSV @20:54:14.32 Allowing 130s for 25.0ml of remaining 975.0ml @20:54:14.33 Intake.select :open @20:54:16.49 Intake @15.2psia, vacuum from 9.7 to 12.7psi @20:54:16.51 SS.reconfigure :inhaling 1.79ml/s @20:54:16.55 SS.seek 25ml @20:54:30.28 Vacuum too high -- waiting up to 40 seconds @20:54:30.29 SS.reconfigure :exhale @20:54:30.33 SS.hold @20:54:31.18 Intake @12.5psia, vacuum from 7.0 to 10.0psi @20:54:31.19 SS.reconfigure :inhaling 1.61ml/s @20:54:31.23 SS.seek 25ml @20:54:35.36 Stroke completed -- waiting for pressure to equalize ... SS.reconfigure :exhale @20:54:35.40 SS.hold @20:54:45.19 Intake.select :close @20:54:45.33 SSV.dial :exhaust @20:54:46.09 Exhaust.select :open @20:54:46.19 SS.seek 0ml @20:54:55.22 Exhaust.select :close @20:54:55.33 SSV.dial CBV @20:54:55.36
CBV.dial SSV @20:54:56.10 Allowing 130s for 25.0ml of remaining 950.0ml @20:54:56.11 Intake.select :open @20:54:58.26 Intake @15.4psia, vacuum from 9.9 to 12.9psi @20:54:58.28 SS.reconfigure :inhaling 1.61ml/s @20:54:58.32 SS.seek 25ml @20:55:13.84 Vacuum too high -- waiting up to 40 seconds @20:55:13.85 SS.reconfigure :exhale @20:55:13.89 SS.hold @20:55:17.02 Intake @13.0psia, vacuum from 7.5 to 10.5psi @20:55:17.03 SS.reconfigure :inhaling 1.45ml/s @20:55:17.07 SS.seek 25ml @20:55:20.78 Stroke completed -- waiting for pressure to equalize ... @20:55:20.79 SS.reconfigure :exhale @20:55:20.83 SS.hold @20:55:28.28 Intake.select :close @20:55:28.42 SSV.dial :exhaust @20:55:29.18 Exhaust.select :open @20:55:29.28 SS.seek 0ml @20:55:38.10 Exhaust.select :close @20:55:38.20 SSV.dial CBV @20:55:38.22
CBV.dial SSV @20:55:38.99 Allowing 130s for 25.0ml of remaining 925.0ml @20:55:39.00 Intake.select :open @20:55:41.15 Intake @15.4psia, vacuum from 9.9 to 12.9psi @20:55:41.17 SS.reconfigure :inhaling 1.45ml/s @20:55:41.21 SS.seek 25ml @20:55:57.03 Vacuum too high -- waiting up to 40 seconds @20:55:57.04 SS.reconfigure :exhale @20:55:57.08 SS.hold @20:56:01.34 Intake @13.4psia, vacuum from 7.9 to 10.9psi @20:56:01.36 SS.reconfigure :inhaling 1.30ml/s @20:56:01.40 SS.seek 25ml @20:56:06.88 Stroke completed -- waiting for pressure to equalize ... @20:56:06.89 SS.reconfigure :exhale @20:56:06.93 SS.hold @20:56:14.59 Intake.select :close @20:56:14.73 SSV.dial :exhaust @20:56:15.49 Exhaust.select :open @20:56:15.60 SS.seek 0ml @20:56:24.44 Exhaust.select :close @20:56:24.54 SSV.dial CBV @20:56:24.57
CBV.dial SSV @20:56:25.33 Allowing 130s for 25.0ml of remaining 900.0ml @20:56:25.34 Intake.select :open @20:56:27.50 Intake @15.3psia, vacuum from 9.8 to 12.8psi @20:56:27.52 SS.reconfigure :inhaling 1.30ml/s @20:56:27.56 SS.seek 25ml @20:56:42.62 Vacuum too high -- waiting up to 40 seconds @20:56:42.63 SS.reconfigure :exhale @20:56:42.67 SS.hold @20:56:47.53 Intake @13.8psia, vacuum from 8.3 to 11.3psi @20:56:47.55 SS.reconfigure :inhaling 1.17ml/s @20:56:47.59 SS.seek 25ml @20:56:55.92 Stroke completed -- waiting for pressure to equalize ... @20:56:55.93 SS.reconfigure :exhale @20:56:55.97 SS.hold @20:57:05.15 Intake.select :close @20:57:05.29 SSV.dial :exhaust @20:57:06.05 Exhaust.select :open @20:57:06.16 SS.seek 0ml @20:57:15.25 Exhaust.select :close @20:57:15.36 SSV.dial CBV @20:57:15.41
CBV.dial SSV @20:57:16.13 Allowing 130s for 25.0ml of remaining 875.0ml @20:57:16.14 Intake.select :open @20:57:18.30 Intake @15.4psia, vacuum from 9.9 to 12.9psi @20:57:18.31 SS.reconfigure :inhaling 1.17ml/s @20:57:18.36 SS.seek 25ml @20:57:35.00 Vacuum too high -- waiting up to 40 seconds @20:57:35.01 SS.reconfigure :exhale @20:57:35.05 SS.hold @20:57:40.67 Intake @14.0psia, vacuum from 8.5 to 11.5psi @20:57:40.69 SS.reconfigure :inhaling 1.06ml/s @20:57:40.73 SS.seek 25ml @20:57:48.92 Stroke completed -- waiting for pressure to equalize ... @20:57:48.93 SS.reconfigure :exhale @20:57:48.97 SS.hold @20:58:00.03 Intake.select :close @20:58:00.17 SSV.dial :exhaust @20:58:00.92 Exhaust.select :open @20:58:01.03 SS.seek 0ml @20:58:10.11 Exhaust.select :close @20:58:10.21 SSV.dial CBV @20:58:10.24
CBV.dial SSV @20:58:11.00 Allowing 130s for 25.0ml of remaining 850.0ml @20:58:11.01 Intake.select :open @20:58:13.17 Intake @15.3psia, vacuum from 9.8 to 12.8psi @20:58:13.19 SS.reconfigure :inhaling 1.06ml/s @20:58:13.23 SS.seek 25ml @20:58:30.37 Vacuum too high -- waiting up to 40 seconds @20:58:30.38 SS.reconfigure :exhale @20:58:30.42 SS.hold @20:58:37.42 Intake @14.3psia, vacuum from 8.8 to 11.8psi @20:58:37.43 SS.reconfigure :inhaling 0.95ml/s @20:58:37.48 SS.seek 25ml @20:58:47.73 Stroke completed -- waiting for pressure to equalize ... @20:58:47.74 SS.reconfigure :exhale @20:58:47.79 SS.hold @20:59:02.05 Intake.select :close @20:59:02.18 SSV.dial :exhaust @20:59:02.94 Exhaust.select :open @20:59:03.05 SS.seek 0ml @20:59:11.87 Exhaust.select :close @20:59:11.98 SSV.dial CBV @20:59:12.00
CBV.dial SSV @20:59:12.77 Allowing 130s for 25.0ml of remaining 825.0ml @20:59:12.78 Intake.select :open @20:59:14.93 Intake @15.4psia, vacuum from 9.9 to 12.9psi @20:59:14.95 SS.reconfigure :inhaling 0.95ml/s @20:59:14.99 SS.seek 25ml @20:59:31.40 Vacuum too high -- waiting up to 40 seconds @20:59:31.41 SS.reconfigure :exhale @20:59:31.45 SS.hold @20:59:38.90 Intake @14.5psia, vacuum from 9.0 to 12.0psi @20:59:38.91 SS.reconfigure :inhaling 0.87ml/s @20:59:38.95 SS.seek 25ml @20:59:53.30 Stroke completed -- waiting for pressure to equalize ... @20:59:53.31 SS.reconfigure :exhale @20:59:53.35 SS.hold @21:00:11.55 Intake.select :close @21:00:11.69 SSV.dial :exhaust @21:00:12.42 Exhaust.select :open @21:00:12.53 SS.seek 0ml @21:00:21.59 Exhaust.select :close @21:00:21.70 SSV.dial CBV @21:00:21.74
CBV.dial SSV @21:00:22.45 Allowing 130s for 25.0ml of remaining 800.0ml @21:00:22.46 Intake.select :open @21:00:24.62 Intake @15.4psia, vacuum from 9.9 to 12.9psi @21:00:24.63 SS.reconfigure :inhaling 0.87ml/s @21:00:24.68 SS.seek 25ml @21:00:40.65 Vacuum too high -- waiting up to 40 seconds @21:00:40.66 SS.reconfigure :exhale @21:00:40.70 SS.hold @21:00:49.26 Intake @14.7psia, vacuum from 9.2 to 12.2psi @21:00:49.28 SS.reconfigure :inhaling 0.78ml/s @21:00:49.33 SS.seek 25ml @21:01:04.46 Vacuum too high -- waiting up to 40 seconds @21:01:04.47 SS.reconfigure :exhale @21:01:04.51 SS.hold @21:01:17.18 Intake @14.9psia, vacuum from 9.4 to 12.4psi @21:01:17.19 SS.reconfigure :inhaling 0.70ml/s @21:01:17.24 SS.seek 25ml @21:01:20.80 Stroke completed -- waiting for pressure to equalize ... @21:01:20.81 SS.reconfigure :exhale @21:01:20.85 SS.hold @21:01:30.59 Intake.select :close @21:01:30.73 SSV.dial :exhaust @21:01:31.47 Exhaust.select :open @21:01:31.57 SS.seek 0ml @21:01:40.70 Exhaust.select :close @21:01:40.81 SSV.dial CBV @21:01:40.82
CBV.dial SSV @21:01:41.58 Allowing 130s for 25.0ml of remaining 775.0ml @21:01:41.59 Intake.select :open @21:01:43.74 Intake @15.2psia, vacuum from 9.7 to 12.7psi @21:01:43.76 SS.reconfigure :inhaling 0.70ml/s @21:01:43.80 SS.seek 25ml @21:02:04.36 Vacuum too high -- waiting up to 40 seconds @21:02:04.37 SS.reconfigure :exhale @21:02:04.41 SS.hold @21:02:15.54 Intake @14.8psia, vacuum from 9.3 to 12.3psi @21:02:15.55 SS.reconfigure :inhaling 0.63ml/s @21:02:15.59 SS.seek 25ml @21:02:34.81 Stroke completed -- waiting for pressure to equalize ... @21:02:34.82 SS.reconfigure :exhale @21:02:34.86 SS.hold @21:02:59.20 Intake.select :close @21:02:59.34 SSV.dial :exhaust @21:03:00.07 Exhaust.select :open @21:03:00.18 SS.seek 0ml @21:03:09.45 Exhaust.select :close @21:03:09.56 SSV.dial CBV @21:03:09.59
CBV.dial SSV @21:03:10.33 Allowing 130s for 25.0ml of remaining 750.0ml @21:03:10.34 Intake.select :open @21:03:12.49 Intake @15.4psia, vacuum from 9.9 to 12.9psi @21:03:12.50 SS.reconfigure :inhaling 0.63ml/s @21:03:12.54 SS.seek 25ml @21:03:34.11 Vacuum too high -- waiting up to 40 seconds @21:03:34.12 SS.reconfigure :exhale @21:03:34.16 SS.hold @21:03:46.83 Intake @14.9psia, vacuum from 9.4 to 12.4psi @21:03:46.84 SS.reconfigure :inhaling 0.57ml/s @21:03:46.89 SS.seek 25ml @21:04:06.39 Vacuum too high -- waiting up to 40 seconds @21:04:06.40 SS.reconfigure :exhale @21:04:06.45 SS.hold @21:04:22.72 Intake @15.2psia, vacuum from 9.7 to 12.7psi @21:04:22.74 SS.reconfigure :inhaling 0.51ml/s @21:04:22.78 SS.seek 25ml @21:04:26.21 Stroke completed -- waiting for pressure to equalize ... @21:04:26.22 SS.reconfigure :exhale @21:04:26.26 SS.hold @21:04:42.44 Intake.select :close @21:04:42.58 SSV.dial :exhaust @21:04:43.32 Exhaust.select :open @21:04:43.43 SS.seek 0ml @21:04:52.46 Exhaust.select :close @21:04:52.57 SSV.dial CBV @21:04:52.58
CBV.dial SSV @21:04:53.34 Allowing 130s for 25.0ml of remaining 725.0ml @21:04:53.35 Intake.select :open @21:04:55.50 Intake @15.4psia, vacuum from 9.9 to 12.9psi @21:04:55.52 SS.reconfigure :inhaling 0.51ml/s @21:04:55.56 SS.seek 25ml @21:05:19.86 Vacuum too high -- waiting up to 40 seconds @21:05:19.87 SS.reconfigure :exhale @21:05:19.91 SS.hold @21:05:34.14 Intake @15.2psia, vacuum from 9.7 to 12.7psi @21:05:34.15 SS.reconfigure :inhaling 0.47ml/s @21:05:34.19 SS.seek 25ml @21:05:55.17 Vacuum too high -- waiting up to 40 seconds @21:05:55.18 SS.reconfigure :exhale @21:05:55.22 SS.hold @21:06:11.48 Intake @15.1psia, vacuum from 9.6 to 12.6psi @21:06:11.50 SS.reconfigure :inhaling 0.43ml/s @21:06:11.54 SS.seek 25ml @21:06:21.24 Stroke completed -- waiting for pressure to equalize ... @21:06:21.25 SS.reconfigure :exhale @21:06:21.29 SS.hold @21:06:45.66 Intake.select :close @21:06:45.79 SSV.dial :exhaust @21:06:46.54 Exhaust.select :open @21:06:47.31 SS.seek 0ml @21:06:56.27 Exhaust.select :close @21:06:56.37 SSV.dial CBV @21:06:56.40
CBV.dial SSV @21:06:57.15 Allowing 130s for 25.0ml of remaining 700.0ml @21:06:57.16 Intake.select :open @21:06:59.31 Intake @15.5psia, vacuum from 10.0 to 13.0psi @21:06:59.33 SS.reconfigure :inhaling 0.43ml/s @21:06:59.37 SS.seek 25ml @21:07:33.89 Vacuum too high -- waiting up to 40 seconds @21:07:33.90 SS.reconfigure :exhale @21:07:33.94 SS.hold @21:07:52.19 Intake @15.2psia, vacuum from 9.7 to 12.7psi @21:07:52.21 SS.reconfigure :inhaling 0.38ml/s @21:07:52.25 SS.seek 25ml @21:08:24.76 Vacuum too high -- waiting up to 40 seconds @21:08:24.77 SS.reconfigure :exhale @21:08:24.81 SS.hold @21:08:49.19 Intake @15.2psia, vacuum from 9.7 to 12.7psi @21:08:49.21 SS.reconfigure :inhaling 0.34ml/s @21:08:49.25 SS.seek 25ml @21:08:49.96 Stroke completed -- waiting for pressure to equalize ... @21:08:49.97 SS.reconfigure :exhale @21:08:50.01 SS.hold @21:09:06.19 Intake.select :close @21:09:06.32 SSV.dial :exhaust @21:09:07.07 Exhaust.select :open @21:09:07.17 SS.seek 0ml @21:09:16.03 Exhaust.select :close @21:09:16.14 SSV.dial CBV @21:09:16.17
CBV.dial SSV @21:09:16.91 Allowing 130s for 25.0ml of remaining 675.0ml @21:09:16.92 Intake.select :open @21:09:19.07 Intake @15.4psia, vacuum from 9.9 to 12.9psi @21:09:19.09 SS.reconfigure :inhaling 0.34ml/s @21:09:19.13 SS.seek 25ml @21:10:13.23 Vacuum too high -- waiting up to 40 seconds @21:10:13.24 SS.reconfigure :exhale @21:10:13.28 SS.hold @21:10:34.12 Intake @15.3psia, vacuum from 9.8 to 12.8psi @21:10:34.14 SS.reconfigure :inhaling 0.31ml/s @21:10:34.18 SS.seek 25ml @21:11:09.25 Stroke completed -- waiting for pressure to equalize ... @21:11:09.26 SS.reconfigure :exhale @21:11:09.30 SS.hold @21:11:26.93 SS.reconfigure :default @21:11:26.97 SS.hold @21:11:27.07 Intake.select :close @21:11:27.22 ShallowSampler::Clogged in MAIN -- Filtering too Slowly after 350.0ml @21:11:27.23 SSV.dial :exhaust @21:11:27.97 Exhaust.select :open @21:11:28.08 SS.reconfigure :exhale @21:11:28.12 SS.seek 0ml @21:11:37.47 Exhaust.select :close @21:11:37.57 Sampled 350.0ml @21:11:37.58 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @21:11:37.59 Intake.select :close @21:11:37.65 CSV.dial :waste @21:11:37.72 CS.seek 0ml @21:11:37.79 SSV.dial CBV,avoiding: :exhaust @21:11:37.81
CBV.dial SSV @21:11:37.87 CSV.dial :air @21:11:38.61 CS.seek 10ml @21:11:39.69
CTV.dial :air @21:11:40.48 delay 15 seconds @21:11:55.50 SSV.dial :spit,avoiding: :exhaust @21:11:56.81 CTV.dial CSV,avoiding: :intake @21:11:56.84
SS.seek 0ml @21:11:56.95 SSV.dial CBV,avoiding: :exhaust @21:12:03.03 CBV.dial between SSV and waste2 @21:12:03.42 CTV.dial CSV,avoiding: :air @21:12:03.43
CSV.dial CTV @21:12:03.88 CS.reconfigure :pressurize @21:12:03.92 CS.seek 0ml @21:12:22.51 BubblePoint: Intake @39.1psia, Outlet @14.9psia @21:12:22.52 CBV.dial SSV @21:12:22.84 SS.reconfigure :default @21:12:22.89 SS.seek 10ml @21:12:28.38 delay 15 seconds @21:12:43.49 Evacuated: Intake @16.2psia, Outlet @9.8psia @21:12:43.50 SSV.dial :spit,avoiding: :exhaust @21:12:44.82 SS.reconfigure :exhale @21:12:44.86 SS.seek 0ml @21:12:50.21 SS.reconfigure :fast @21:12:50.25 SS.seek 5ml @21:12:53.10 SSV.dial CBV,avoiding: :exhaust @21:12:53.12
CBV.dial SSV @21:12:54.44 CTV.dial :air,avoiding: :intake @21:12:55.81 CSV.dial CTV @21:12:55.84
SS.seek 10ml @21:12:56.84 CSV.dial :air @21:12:57.30 CS.reconfigure :fast @21:12:57.34 CS.seek 0ml @21:12:58.33
SSV.dial :spit,avoiding: :exhaust @21:12:59.65 SS.reconfigure :exhale @21:12:59.69 SS.seek 0ml @21:13:04.85 CS.stop @21:13:05.02
SS.reconfigure :fast @21:13:05.07 SS.seek 5ml @21:13:07.88 SSV.dial CBV,avoiding: :exhaust @21:13:07.90
CBV.dial SSV @21:13:10.23 SSV.dial :spit,avoiding: :exhaust @21:13:11.55 SS.reconfigure :default @21:13:11.59 SS.seek 0ml @21:13:14.95 SS.stop @21:13:14.99 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @21:13:15.00 Intake.select :close @21:13:15.07 CSV.dial :waste @21:13:15.17 SSV.dial CBV,avoiding: :exhaust @21:13:15.19
CBV.dial SSV @21:13:15.83 CS.seek 0ml @21:13:15.89 CSV.dial :air @21:13:16.53
CTV.dial :air @21:13:16.55 delay 15 seconds @21:13:16.62 CS.seek 10ml @21:13:31.56
SSV.dial :spit,avoiding: :exhaust @21:13:32.90 CTV.dial CSV,avoiding: :intake @21:13:32.94
SS.reconfigure :exhale @21:13:33.01 SS.seek 0ml @21:13:33.44 SSV.dial CBV,avoiding: :exhaust @21:13:41.35 CBV.dial between SSV and waste2 @21:13:41.73 CTV.dial CSV,avoiding: :air @21:13:41.75
CSV.dial CTV @21:13:42.19 CS.reconfigure :pressurize @21:13:42.23 CS.seek 0ml @21:14:03.67 BubblePoint: Intake @38.0psia, Outlet @13.9psia CBV.dial SSV @21:14:04.00 SS.reconfigure :default @21:14:04.04 SS.seek 10ml @21:14:09.64 delay 15 seconds @21:14:24.76 Evacuated: Intake @18.0psia, Outlet @11.4psia @21:14:24.77 SSV.dial :spit,avoiding: :exhaust @21:14:26.10 SS.reconfigure :exhale @21:14:26.14 SS.seek 0ml @21:14:31.57 SS.reconfigure :fast @21:14:31.61 SS.seek 5ml @21:14:34.48 SSV.dial CBV,avoiding: :exhaust @21:14:34.49
CBV.dial SSV @21:14:35.81 CTV.dial :air,avoiding: :intake @21:14:37.15 CSV.dial CTV @21:14:37.19
SS.seek 10ml @21:14:38.18 CSV.dial :air @21:14:38.64 CS.reconfigure :fast @21:14:38.68 CS.seek 0ml @21:14:39.78
SSV.dial :spit,avoiding: :exhaust @21:14:41.10 SS.reconfigure :exhale @21:14:41.14 SS.seek 0ml @21:14:43.69 CS.stop @21:14:46.37
SS.reconfigure :fast @21:14:46.41 SS.seek 5ml @21:14:49.28 SSV.dial CBV,avoiding: :exhaust @21:14:49.29
CBV.dial SSV @21:14:51.62 SSV.dial :spit,avoiding: :exhaust @21:14:52.93 SS.reconfigure :default @21:14:52.97 SS.seek 0ml @21:14:56.37 SS.stop @21:14:56.41 Lysing: Adding 2.0ml AqMeOH @21:14:56.42 CBV.dial between CSV and waste1,avoiding: :DBV @21:14:57.90 CTV.dial CSV @21:14:59.21 CSV.dial CSR @21:15:00.00 Pulling 1ml of CSR.AqMeOH @21:15:00.01 CSR.select :AqMeOH @21:15:00.11 CS.reconfigure :default @21:15:00.15 CS.seek 1ml @21:15:03.76 delay 5 seconds @21:15:08.78 CSR.select :air @21:15:08.93 CS.seek 6ml @21:15:22.54 delay 2 seconds @21:15:24.55 CSV.dial :waste2 @21:15:25.00 CS.reconfigure :fast @21:15:25.04 CS.seek 0ml @21:15:39.44 CSV.dial CSR @21:15:39.94 Pulling 2ml of CSR.AqMeOH @21:15:39.95 CSR.select :AqMeOH @21:15:40.05 CS.reconfigure :default @21:15:40.09 CS.seek 2ml @21:15:46.10 delay 5 seconds @21:15:51.12 CSR.select :air @21:15:51.27 CS.seek 5ml @21:15:59.94 delay 2 seconds @21:16:01.96 CSV.dial CTV @21:16:02.39 CS.seek 1ml @21:16:13.73 Before lysing Collection Puck Heater at 16.4C/38.5psia @21:16:13.75 Heating Puck.from 3, :sh1 in Collection Clamp to 70C for 10:00 @21:16:13.77 CH.seek 70C,8:00 @21:18:03.80 CH.hold 10:00,70C @21:18:03.98 Begun lysing Collection Puck Heater at 68C/47.0psia @21:28:03.99 Ended lysing Collection Puck Heater at 70C/46.3psia @21:28:04.00 CS.seek 0ml @21:28:07.59 CTV.dial between spare and air,avoiding: :intake @21:28:08.62 CSV.dial :air @21:28:09.07 CS.seek 5ml @21:28:22.87 CSV.dial CTV @21:28:23.31 delay 2 seconds @21:28:25.32 CSV.dial :air @21:28:25.78 delay 2 seconds @21:28:27.79 CSV.dial CTV @21:28:28.25 delay 2 seconds @21:28:30.26 CSV.dial :air @21:28:30.71 delay 2 seconds @21:28:32.74 CS.seek 0ml @21:28:46.39 Puck @74.0psia after final lysis pressurization @21:28:46.40 Recovering Lysate into Collection syringe @21:28:46.41 CSV.dial CBV @21:28:47.40 CBV.dial CSV @21:28:47.76 CS.seek 5ml @21:29:01.52 delay 5 seconds @21:29:06.54 CTV.dial :air @21:29:07.05 CS.seek 6.999ml @21:29:13.12 delay 5 seconds @21:29:18.14 CSV.dial :air2 @21:29:18.58 CS.seek 10ml @21:29:27.15 CSV.dial between air and CTV,avoiding: CBV @21:29:28.31 Lysing Complete @21:29:28.38 Storing sh1 puck in Collection Clamp to tube 2 @21:29:28.54 SC.reconfigure :tight @21:29:28.58 SC.seek 2 @21:29:32.14 SC.configure :limp @21:29:32.33 Elbow.reconfigure :tight @21:29:32.38 Elbow.seek 2 @21:29:34.08 Forearm.reconfigure :tight @21:29:34.12 Forearm.seek 2 + 450 counts @21:29:35.17 SE.reconfigure :pick @21:29:35.22 SE.seek :beamBreak @21:29:36.53 SE.configure :default @21:29:36.67 Elbow.reconfigure :limp @21:29:36.72 Forearm.reconfigure :loose @21:29:36.75 Forearm.seek 2 @21:29:37.02 Elbow.hold @21:29:37.08 Elbow.reconfigure :tight @21:29:37.17 SE.seek 26.1 Pucks below Top Plate @21:29:37.71 Forearm.force -20 @21:29:37.74 Forearm.seek 2 + 280 counts @21:29:38.02 Forearm.reconfigure :tight @21:29:38.06 Forearm.seek 2 + 450 counts @21:29:38.26 SE.seek 27.1 Pucks below Top Plate @21:29:38.89 Forearm.reconfigure :loose @21:29:38.93 Forearm.seek :retract @21:29:39.83 Elbow.seek CC @21:29:41.52 Elbow.reconfigure :loose @21:29:41.61 Forearm.seek CC @21:29:42.59 Hand.close @21:29:43.18 CC.seek :open @21:29:47.89 Forearm.seek :retract @21:29:49.33 Elbow.reconfigure :tight @21:29:49.39 Elbow.seek 2 @21:29:51.17 Elbow.reconfigure :limp @21:29:51.22 Forearm.seek 2 @21:29:52.21 Elbow.hold @21:29:52.27 Elbow.reconfigure :tight @21:29:52.31 Hand.open @21:29:53.05 Forearm.force -20 @21:29:53.08 Forearm.seek 2 + 314 counts @21:29:53.46 Forearm.reconfigure :tight @21:29:53.50 Forearm.seek 2 + 450 counts @21:29:53.85 SE.seek :down @21:29:54.73 SE.stop @21:29:54.97
Forearm.reconfigure :loose @21:29:55.02 Forearm.seek :retract @21:29:55.94 Tranferring DA @21:29:55.95 Beginning samplePassC @21:29:55.97 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @21:29:56.15 Elbow.seek FlushPuck::Garage @21:29:58.01 Elbow.reconfigure :loose @21:29:58.11 Forearm.seek :garage @21:29:59.03 Hand.close @21:29:59.78 Forearm.seek :retract @21:30:00.76 Elbow.reconfigure :tight @21:30:00.80 Elbow.seek CC @21:30:03.76 Elbow.reconfigure :loose @21:30:03.85 Forearm.reconfigure :tight @21:30:03.89 Forearm.seek CC @21:30:05.13 CC.seek :closed @21:30:09.63 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.30 @21:30:09.70 Hand.open @21:30:10.36 Forearm.reconfigure :loose @21:30:10.40 Forearm.seek :retract @21:30:11.36 PSV.dial PSR @21:30:12.74 Pulling 1.5ml of PSR.flush @21:30:12.75 PSR.select :flush @21:30:12.87 PS.seek 1.5ml @21:30:29.10 delay 5 seconds @21:30:34.12 PSV.dial :waste1 @21:30:34.57 PS.seek 0ml @21:30:50.78 PSV.dial PSR @21:30:51.23 PSR.select :air @21:30:51.34 PS.seek 2.5ml @21:31:17.76 PRV.dial CBV,avoiding: :ab2 @21:31:17.77
CBV.dial PRV @21:31:19.45 PSV.dial :mixloop @21:31:20.49 CTV.dial :air,avoiding: :intake @21:31:20.50 PTV.dial :PRVmixing @21:31:21.82 PS.seek 0ml @21:31:48.15 PSV.dial :air @21:31:48.72 PS.seek 2.5ml @21:32:15.06 PSV.dial :mixloop @21:32:15.51 PS.seek 0ml @21:32:41.87 PSV.dial :air @21:32:42.33 PS.seek 2.5ml @21:33:08.62 PSV.dial :mixloop @21:33:09.08 PS.seek 0ml @21:33:35.47 Garaging FlushPuck in Collection Clamp @21:33:35.53 Elbow.reconfigure :tight @21:33:35.58 Elbow.seek CC @21:33:35.91 Elbow.reconfigure :loose @21:33:36.00 Forearm.seek CC @21:33:37.03 Hand.close @21:33:37.67 CC.seek :open @21:33:42.31 Forearm.seek :retract @21:33:43.76 Elbow.reconfigure :tight @21:33:43.81 Elbow.seek :garage @21:33:46.63 Elbow.reconfigure :loose @21:33:46.72 Forearm.reconfigure :tight @21:33:46.76 Forearm.seek :garage @21:33:47.78 Hand.open @21:33:48.48 Forearm.reconfigure :loose @21:33:48.52 Forearm.seek :retract @21:33:49.53 PSV.dial :mixloop @21:33:49.54 PTV.dial :PRVmixing @21:33:49.56 PRV.dial CBV @21:33:49.59
CBV.dial PRV @21:33:49.61 CTV.dial :air,avoiding: :intake @21:33:49.62 Loading lyfil puck from tube 3 into Collection Clamp @21:33:49.85 SC.reconfigure :tight @21:33:49.89 SC.seek 3 @21:33:53.48 SC.configure :limp @21:33:53.67 Elbow.reconfigure :tight @21:33:53.71 Elbow.seek 3 @21:33:55.63 Forearm.reconfigure :tight @21:33:55.67 Forearm.seek 3 + 450 counts @21:33:56.69 SE.reconfigure :pick @21:33:56.74 SE.seek :beamBreak @21:33:59.65 SE.configure :default @21:33:59.79 Elbow.seek 3 @21:33:59.96 Forearm.seek 3 + 300 counts @21:34:00.22 Forearm.reconfigure :loose @21:34:00.26 Hand.close @21:34:00.94 SE.seek :down @21:34:01.12
Forearm.seek :retract @21:34:02.03 Elbow.seek CC @21:34:03.48 SE.stop @21:34:03.68
Elbow.reconfigure :loose @21:34:03.77 Forearm.reconfigure :tight @21:34:03.82 Forearm.seek CC @21:34:04.99 CC.seek :closed @21:34:09.50 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.15 @21:34:09.51 Hand.open @21:34:10.24 Forearm.reconfigure :loose @21:34:10.28 Forearm.seek :retract @21:34:11.22 CTV.dial CSV,avoiding: :intake @21:34:12.59 CSV.dial CTV @21:34:12.92 PS.seek 2.5ml @21:34:39.30 CS.seek 4.501ml @21:34:54.21 delay 8 seconds @21:35:02.23 PSV.dial :air @21:35:02.69 delay 2 seconds @21:35:04.70 PSV.dial :waste2 @21:35:05.44 PS.seek 0ml @21:35:31.81 CBV.dial between PRV and waste1 @21:35:32.13 CSV.dial :air @21:35:32.59 CS.seek 10ml @21:35:47.45 CSV.dial CTV @21:35:47.90 CS.seek 0ml @21:36:14.27 CBV.dial :waste1 @21:36:14.56 delay 5 seconds @21:36:19.59 CTV.dial :air,avoiding: :intake @21:36:20.98 Storing lyfil puck in Collection Clamp to tube 2 @21:36:21.14 SC.reconfigure :tight @21:36:21.18 SC.seek 2 @21:36:24.78 SC.configure :limp @21:36:24.96 Elbow.reconfigure :tight @21:36:25.01 Elbow.seek 2 @21:36:26.75 Forearm.reconfigure :tight @21:36:27.27 Forearm.seek 2 + 450 counts @21:36:28.29 SE.reconfigure :pick @21:36:28.34 SE.seek :beamBreak @21:36:29.64 SE.configure :default @21:36:29.81 Elbow.reconfigure :limp @21:36:29.86 Forearm.reconfigure :loose @21:36:29.90 Forearm.seek 2 @21:36:30.12 Elbow.hold @21:36:30.18 Elbow.reconfigure :tight @21:36:30.27 SE.seek 27.09 Pucks below Top Plate @21:36:30.79 Forearm.force -20 @21:36:30.82 Forearm.seek 2 + 307 counts @21:36:31.10 Forearm.reconfigure :tight @21:36:31.14 Forearm.seek 2 + 450 counts @21:36:31.44 SE.seek 28.09 Pucks below Top Plate @21:36:32.06 Forearm.reconfigure :loose @21:36:32.10 Forearm.seek :retract @21:36:33.02 Elbow.seek CC @21:36:34.71 Elbow.reconfigure :loose @21:36:34.81 Forearm.seek CC @21:36:35.76 Hand.close @21:36:36.42 CC.seek :open @21:36:41.13 Forearm.seek :retract @21:36:42.13 Elbow.reconfigure :tight @21:36:42.17 Elbow.seek 2 @21:36:43.90 Elbow.reconfigure :limp @21:36:43.95 Forearm.seek 2 @21:36:44.97 Elbow.hold @21:36:45.03 Elbow.reconfigure :tight @21:36:45.07 Hand.open @21:36:45.78 Forearm.force -20 @21:36:45.81 Forearm.seek 2 + 302 counts @21:36:46.10 Forearm.reconfigure :tight @21:36:46.15 Forearm.seek 2 + 450 counts @21:36:46.41 SE.seek :down @21:36:47.27 SE.stop @21:36:47.52
Forearm.reconfigure :loose @21:36:47.57 Forearm.seek :retract @21:36:48.47 PSV.dial :air @21:36:49.22 PS.seek 2.5ml @21:37:15.59 delay 1 second @21:37:16.60 PSV.dial :mixloop @21:37:17.04 delay 1 second @21:37:18.06 PSV.dial :air @21:37:18.63 delay 1 second @21:37:19.65 PSV.dial :mixloop @21:37:20.09 delay 1 second @21:37:21.10 PSV.dial :air @21:37:21.57 delay 1 second @21:37:22.59 PS.seek 0ml @21:37:49.00 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @21:37:49.16 Elbow.seek FlushPuck::Garage @21:37:51.02 Elbow.reconfigure :loose @21:37:51.11 Forearm.seek :garage @21:37:52.14 Hand.close @21:37:52.90 Forearm.seek :retract @21:37:53.91 Elbow.reconfigure :tight @21:37:53.95 Elbow.seek PC @21:37:55.18 Elbow.reconfigure :loose @21:37:55.27 Forearm.reconfigure :tight @21:37:55.31 Forearm.seek PC @21:37:56.50 PC.seek :closed @21:38:01.12 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.56 @21:38:01.18 Hand.open @21:38:01.85 Forearm.reconfigure :loose @21:38:01.90 Forearm.seek :retract @21:38:02.87 PRV.dial :air @21:38:03.32 PSV.dial :mixloop @21:38:03.85 PS.seek 0.3ml @21:38:07.87 PSV.dial PSR @21:38:08.97 Pulling 0.4ml of PSR.air @21:38:08.98 PSR.select :air @21:38:09.00 PS.seek 0.7ml @21:38:13.87 delay 3 seconds @21:38:16.88 PSR.select :air @21:38:16.90 PS.seek 2.5ml @21:38:36.09 delay 3 seconds @21:38:39.10 PSV.dial :mixloop @21:38:40.27 PTV.dial :mixing @21:38:41.02 PBV.dial :waste2 @21:38:41.97 PS.seek 0ml @21:39:08.31 Garaging FlushPuck in Processing Clamp @21:39:08.38 Elbow.reconfigure :tight @21:39:08.42 Elbow.seek PC @21:39:08.52 Elbow.reconfigure :loose @21:39:08.61 Forearm.seek PC @21:39:09.59 Hand.close @21:39:10.17 PC.seek :open @21:39:14.88 Forearm.seek :retract @21:39:15.84 Elbow.reconfigure :tight @21:39:15.88 Elbow.seek :garage @21:39:17.24 Elbow.reconfigure :loose @21:39:17.33 Forearm.reconfigure :tight @21:39:17.37 Forearm.seek :garage @21:39:18.32 Hand.open @21:39:18.98 Forearm.reconfigure :loose @21:39:19.02 Forearm.seek :retract @21:39:19.99 Loading da puck from tube 3 into Processing Clamp @21:39:20.21 SC.reconfigure :tight @21:39:20.25 SC.seek 3 @21:39:23.82 SC.configure :limp @21:39:24.00 Elbow.reconfigure :tight @21:39:24.05 Elbow.seek 3 @21:39:25.90 Forearm.reconfigure :tight @21:39:25.94 Forearm.seek 3 + 450 counts @21:39:26.98 SE.reconfigure :pick @21:39:27.02 SE.seek :beamBreak @21:39:29.93 SE.configure :default @21:39:30.08 Elbow.seek 3 @21:39:30.24 Forearm.seek 3 + 300 counts @21:39:30.50 Forearm.reconfigure :loose @21:39:30.54 Hand.close @21:39:31.19 SE.seek :down @21:39:31.37
Forearm.seek :retract @21:39:32.29 Elbow.seek PC @21:39:33.74 SE.stop @21:39:34.81
Elbow.reconfigure :loose @21:39:34.91 Forearm.reconfigure :tight @21:39:34.95 Forearm.seek PC @21:39:36.20 PC.seek :closed @21:39:40.80 Processing Clamp onSpring/offSpring for 16/26 samples, on/off torque 1.61 @21:39:40.81 Hand.open @21:39:41.47 Forearm.reconfigure :loose @21:39:41.51 Forearm.seek :retract @21:39:42.44 Heating Puck.from 3, :da in Processing Clamp to 28C for 2:00:00 @21:39:42.46 PH.seek 28C,5:00 @21:40:21.74 PH.hold 2:00:00,28C @21:40:21.80 delay 1:30 @21:41:51.83 PSR.select :air @21:41:51.84 PSV.dial PSR @21:41:52.92 Pulling 0.5ml of PSR.wash @21:41:52.93 PSR.select :wash @21:41:53.04 PS.seek 0.5ml @21:41:59.02 delay 5 seconds @21:42:04.04 PSR.select :air @21:42:04.15 PS.seek 2ml @21:42:20.24 PTV.dial :puck @21:42:20.97 PSV.dial PTV @21:42:21.85 PBV.dial :waste1 @21:42:23.24 PS.seek 1.5ml @21:42:29.21 delay 3 seconds @21:42:32.22 PBV.dial between PSV and waste1 @21:42:32.59 PS.seek 0ml @21:42:48.65 delay 5:00 @21:47:48.67 recoverPSRreagent08 @21:47:48.68 PTV.dial :puck @21:47:48.69 PBV.dial :waste1 @21:47:49.06 delay 30 seconds @21:48:19.08 PBV.dial between PSV and waste1 @21:48:19.45 PSR.select :air @21:48:19.47 PSV.dial PSR @21:48:20.22 PS.reconfigure :fast @21:48:20.26 PS.seek 2.5ml @21:48:43.98 PSV.dial PTV @21:48:44.73 delay 5 seconds @21:48:49.75 PSV.dial PSR @21:48:50.51 delay 5 seconds @21:48:55.53 PSV.dial PTV @21:48:56.30 delay 5 seconds @21:49:01.32 PSV.dial :air @21:49:02.07 PS.seek 0ml @21:49:25.04 PTV.dial :PRVmixing,via: PRV @21:49:26.37 PSV.dial :mixloop @21:49:26.82 PRV.dial :ab1 @21:49:27.58 PS.reconfigure :slow @21:49:27.62 PS.seek 0.15ml @21:49:31.39 delay 2 seconds @21:49:33.40 CBV.dial PRV @21:49:33.43
PRV.dial CBV @21:49:33.98 PS.seek 0.35ml @21:49:38.54 delay 2 seconds @21:49:40.56 CBV.dial between PRV and PSV @21:49:40.88 PRV.dial :air @21:49:41.31 PS.seek 0.9ml @21:49:53.01 PRV.dial :waste2 @21:49:53.75 PS.seek 0.5ml @21:50:02.39 addPMLReagent08 PSV.dial :air @21:50:02.86 PS.reconfigure :default @21:50:02.90 PS.seek 2ml @21:50:19.07 PSV.dial :PML @21:50:19.53 PTV.dial :mixing @21:50:20.26 PBV.dial :waste1 @21:50:20.68 PS.seek 1.85ml @21:50:23.07 PBV.dial between PSV and waste1 @21:50:23.46 PS.seek 0ml @21:50:43.17 delay 2 seconds @21:50:45.18 Beginning samplePassC @21:50:45.20 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @21:50:45.36 Elbow.reconfigure :tight @21:50:45.41 Elbow.seek FlushPuck::Garage @21:50:46.74 Elbow.reconfigure :loose @21:50:46.83 Forearm.seek :garage @21:50:47.80 Hand.close @21:50:48.53 Forearm.seek :retract @21:50:49.54 Elbow.reconfigure :tight @21:50:49.58 Elbow.seek CC @21:50:52.48 Elbow.reconfigure :loose @21:50:52.57 Forearm.reconfigure :tight @21:50:52.61 Forearm.seek CC @21:50:53.91 CC.seek :closed @21:50:58.43 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.40 @21:50:58.50 Hand.open @21:50:59.23 Forearm.reconfigure :loose @21:50:59.27 Forearm.seek :retract @21:51:00.19 PSV.dial PSR @21:51:01.28 Pulling 1.5ml of PSR.flush @21:51:01.29 PSR.select :flush @21:51:01.41 PS.seek 1.5ml @21:51:17.55 delay 5 seconds @21:51:22.57 PSV.dial :waste1 @21:51:23.16 PS.seek 0ml @21:51:39.32 PSV.dial PSR @21:51:39.77 PSR.select :air @21:51:39.89 PS.seek 2.5ml @21:52:06.27 PRV.dial CBV,avoiding: :ab2 @21:52:06.28
CBV.dial PRV @21:52:07.92 PSV.dial :mixloop @21:52:08.95 CTV.dial :air,avoiding: :intake @21:52:08.96 PTV.dial :PRVmixing @21:52:09.72 PS.seek 0ml @21:52:36.10 PSV.dial :air @21:52:36.56 PS.seek 2.5ml @21:53:03.01 PSV.dial :mixloop @21:53:03.47 PS.seek 0ml @21:53:29.85 PSV.dial :air @21:53:30.31 PS.seek 2.5ml @21:53:56.72 PSV.dial :mixloop @21:53:57.18 PS.seek 0ml @21:54:23.57 Garaging FlushPuck in Collection Clamp @21:54:23.64 Elbow.reconfigure :tight @21:54:23.68 Elbow.seek CC @21:54:23.98 Elbow.reconfigure :loose @21:54:24.06 Forearm.seek CC @21:54:25.03 Hand.close @21:54:25.66 CC.seek :open @21:54:30.27 Forearm.seek :retract @21:54:31.22 Elbow.reconfigure :tight @21:54:31.26 Elbow.seek :garage @21:54:34.15 Elbow.reconfigure :loose @21:54:34.24 Forearm.reconfigure :tight @21:54:34.28 Forearm.seek :garage @21:54:35.22 Hand.open @21:54:35.84 Forearm.reconfigure :loose @21:54:35.88 Forearm.seek :retract @21:54:36.83 delayUntil "21:58:45.17" @21:54:36.86 Whole Cell Archive sampling 1000ml @21:54:36.88 Loading wcr puck from tube 3 into Collection Clamp @21:54:37.31 Elbow.reconfigure :tight @21:54:37.36 Elbow.seek 3 @21:54:39.30 Forearm.reconfigure :tight @21:54:39.35 Forearm.seek 3 + 450 counts @21:54:40.38 SE.reconfigure :pick @21:54:40.44 SE.seek :beamBreak @21:54:43.40 SE.configure :default @21:54:43.56 Elbow.seek 3 @21:54:43.73 Forearm.seek 3 + 300 counts @21:54:44.17 Forearm.reconfigure :loose @21:54:44.21 Hand.close @21:54:44.80 SE.seek :down @21:54:44.98 Forearm.seek :retract @21:54:45.93 Elbow.seek CC @21:54:47.49 SE.stop @21:54:47.59 Elbow.reconfigure :loose @21:54:48.13 Forearm.reconfigure :tight @21:54:48.20 Forearm.seek CC @21:54:49.47 CC.seek :closed @21:54:53.97 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.51 @21:54:53.99 Hand.open @21:54:54.59 Forearm.reconfigure :loose @21:54:54.64 Forearm.seek :retract @21:54:55.68 collectSample 1000ml w/bubble point of 35psi @21:54:55.69 Priming 7ml @21:54:55.70 Intake.select :close @21:54:55.71 Exhaust.select :close @21:54:55.72 SS.hold @21:54:55.91 Calibrating pressure sensors @21:54:55.92 CTV.dial :air @21:54:55.93 SSV.dial :spit,avoiding: :exhaust @21:54:55.95 SS.reconfigure :fast @21:54:56.00 SS.seek 5ml @21:54:58.88 SSV.dial CBV @21:54:58.92 CBV.dial SSV @21:55:00.20 delay 3.8 seconds @21:55:04.12 SSV.dial :spit @21:55:05.43 SS.seek 0ml @21:55:08.15 SSV.dial CBV @21:55:09.45 CTV.dial :intake @21:55:10.22 SSV.dial CBV @21:55:10.25 CBV.dial SSV @21:55:10.26 Allowing 40s for 7.0ml of remaining 7.0ml @21:55:10.27 Intake.select :open @21:55:12.43 Intake @15.5psia, vacuum from 10.0 to 13.0psi @21:55:12.45 SS.reconfigure :inhaling 2.20ml/s @21:55:12.49 SS.seek 7ml @21:55:19.11 Stroke completed -- waiting for pressure to equalize ... @21:55:19.12 SS.reconfigure :exhale @21:55:19.16 SS.hold @21:55:30.28 Intake.select :close @21:55:30.42 SSV.dial :exhaust @21:55:31.17 Exhaust.select :open @21:55:31.28 SS.seek 0ml @21:55:35.54 Exhaust.select :close @21:55:35.65 Sampling 1000.0ml @21:55:35.66 Intake.select :close @21:55:35.67 Exhaust.select :close @21:55:35.68 SS.reconfigure :default @21:55:35.72 SS.hold @21:55:35.91 CTV.dial :intake @21:55:35.93 SSV.dial CBV @21:55:35.95 CBV.dial SSV @21:55:36.70 Allowing 130s for 25.0ml of remaining 1000.0ml @21:55:36.72 Intake.select :open @21:55:38.87 Intake @16.3psia, vacuum from 10.0 to 13.0psi @21:55:38.89 SS.reconfigure :inhaling 2.20ml/s @21:55:38.93 SS.seek 25ml @21:55:43.96 Vacuum too high -- waiting up to 40 seconds @21:55:43.97 SS.reconfigure :exhale @21:55:44.01 SS.hold @21:55:47.32 Intake @16.1psia, vacuum from 10.0 to 13.0psi @21:55:47.34 SS.reconfigure :inhaling 1.98ml/s @21:55:47.38 SS.seek 25ml @21:55:51.96 Vacuum too high -- waiting up to 40 seconds @21:55:51.97 SS.reconfigure :exhale @21:55:52.01 SS.hold @21:55:56.53 Intake @16.4psia, vacuum from 10.0 to 13.0psi @21:55:56.55 SS.reconfigure :inhaling 1.79ml/s @21:55:56.59 SS.seek 25ml @21:56:00.87 Vacuum too high -- waiting up to 40 seconds @21:56:00.88 SS.reconfigure :exhale @21:56:00.92 SS.hold @21:56:04.63 SS.reconfigure :inhaling 1.61ml/s @21:56:04.67 SS.seek 25ml @21:56:09.01 Stroke completed -- waiting for pressure to equalize ... @21:56:09.02 SS.reconfigure :exhale @21:56:09.07 SS.hold @21:56:15.50 Intake.select :close @21:56:15.64 SSV.dial :exhaust @21:56:16.40 Exhaust.select :open @21:56:16.51 SS.seek 0ml @21:56:25.51 Exhaust.select :close @21:56:25.62 SSV.dial CBV @21:56:25.64 CBV.dial SSV @21:56:26.42 Allowing 130s for 25.0ml of remaining 975.0ml @21:56:26.43 Intake.select :open @21:56:28.59 Intake @16.6psia, vacuum from 10.0 to 13.0psi @21:56:28.61 SS.reconfigure :inhaling 1.61ml/s @21:56:28.65 SS.seek 25ml @21:56:34.89 Vacuum too high -- waiting up to 40 seconds @21:56:34.91 SS.reconfigure :exhale @21:56:34.95 SS.hold @21:56:37.58 Intake @16.0psia, vacuum from 10.0 to 13.0psi @21:56:37.59 SS.reconfigure :inhaling 1.45ml/s @21:56:37.64 SS.seek 25ml @21:56:45.30 Vacuum too high -- waiting up to 40 seconds @21:56:45.31 SS.reconfigure :exhale @21:56:45.35 SS.hold @21:56:50.51 Intake @16.2psia, vacuum from 10.0 to 13.0psi @21:56:50.53 SS.reconfigure :inhaling 1.30ml/s @21:56:50.57 SS.seek 25ml @21:56:57.64 Stroke completed -- waiting for pressure to equalize ... @21:56:57.65 SS.reconfigure :exhale @21:56:57.69 SS.hold @21:57:05.15 Intake.select :close @21:57:05.29 SSV.dial :exhaust @21:57:06.04 Exhaust.select :open @21:57:06.15 SS.seek 0ml @21:57:15.73 Exhaust.select :close @21:57:15.84 SSV.dial CBV @21:57:15.87 CBV.dial SSV @21:57:16.61 Allowing 130s for 25.0ml of remaining 950.0ml @21:57:16.62 Intake.select :open @21:57:18.77 Intake @16.4psia, vacuum from 10.0 to 13.0psi @21:57:18.79 SS.reconfigure :inhaling 1.30ml/s @21:57:18.84 SS.seek 25ml @21:57:27.83 Vacuum too high -- waiting up to 40 seconds @21:57:27.84 SS.reconfigure :exhale @21:57:27.89 SS.hold @21:57:30.96 Intake @15.3psia, vacuum from 9.8 to 12.8psi @21:57:30.98 SS.reconfigure :inhaling 1.17ml/s @21:57:31.02 SS.seek 25ml @21:57:45.48 Stroke completed -- waiting for pressure to equalize ... @21:57:45.49 SS.reconfigure :exhale @21:57:45.53 SS.hold @21:57:55.29 Intake.select :close @21:57:55.44 SSV.dial :exhaust @21:57:56.19 Exhaust.select :open @21:57:56.30 SS.seek 0ml @21:58:05.28 Exhaust.select :close @21:58:05.38 SSV.dial CBV @21:58:05.40 CBV.dial SSV @21:58:06.15 Allowing 130s for 25.0ml of remaining 925.0ml @21:58:06.17 Intake.select :open @21:58:08.32 Intake @16.4psia, vacuum from 10.0 to 13.0psi @21:58:08.34 SS.reconfigure :inhaling 1.17ml/s @21:58:08.38 SS.seek 25ml @21:58:18.08 Vacuum too high -- waiting up to 40 seconds @21:58:18.09 SS.reconfigure :exhale @21:58:18.13 SS.hold @21:58:21.14 Intake @15.6psia, vacuum from 10.0 to 13.0psi @21:58:21.16 SS.reconfigure :inhaling 1.06ml/s @21:58:21.20 SS.seek 25ml @21:58:37.21 Stroke completed -- waiting for pressure to equalize ... @21:58:37.22 SS.reconfigure :exhale @21:58:37.26 SS.hold @21:58:45.18 recoverPRVreagent08 @21:58:45.19 PTV.dial :mixing @21:58:45.95 PBV.dial :waste1 @21:58:46.32 delay 30 seconds @21:58:47.02 Intake.select :close @21:58:47.16 SSV.dial :exhaust @21:58:47.92 Exhaust.select :open @21:58:48.03 SS.seek 0ml @21:58:57.12 Exhaust.select :close @21:58:57.22 SSV.dial CBV @21:58:57.28 CBV.dial SSV @21:58:58.00 Allowing 130s for 25.0ml of remaining 900.0ml @21:58:58.01 Intake.select :open @21:59:00.16 Intake @16.4psia, vacuum from 10.0 to 13.0psi @21:59:00.18 SS.reconfigure :inhaling 1.06ml/s @21:59:00.22 SS.seek 25ml @21:59:13.08 Vacuum too high -- waiting up to 40 seconds @21:59:13.09 SS.reconfigure :exhale @21:59:13.13 SS.hold @21:59:16.40 PBV.dial between PSV and waste1 @21:59:16.77 PSV.dial :air @21:59:17.02 Intake @16.0psia, vacuum from 10.0 to 13.0psi @21:59:17.04 SS.reconfigure :inhaling 0.95ml/s @21:59:17.08 SS.seek 25ml @21:59:17.24 PS.reconfigure :fast @21:59:17.29 PS.seek 2.5ml @21:59:31.63 Stroke completed -- waiting for pressure to equalize ... @21:59:31.65 SS.reconfigure :exhale @21:59:31.69 SS.hold @21:59:41.06 PSV.dial :mixloop @21:59:41.35 Intake.select :close @21:59:41.50 SSV.dial :exhaust @21:59:41.56 delay 2 seconds @21:59:42.27 Exhaust.select :open @21:59:42.38 SS.seek 0ml @21:59:43.58 PSV.dial :air @21:59:44.03 delay 2 seconds @21:59:46.05 PSV.dial :mixloop @21:59:46.50 delay 2 seconds @21:59:48.52 PSV.dial :air @21:59:48.99 PS.seek 0ml @21:59:51.38 Exhaust.select :close @21:59:51.49 SSV.dial CBV @21:59:51.51 CBV.dial SSV @21:59:52.28 Allowing 130s for 25.0ml of remaining 875.0ml @21:59:52.29 Intake.select :open @21:59:54.45 Intake @16.3psia, vacuum from 10.0 to 13.0psi @21:59:54.47 SS.reconfigure :inhaling 0.95ml/s @21:59:54.51 SS.seek 25ml @22:00:09.36 Vacuum too high -- waiting up to 40 seconds @22:00:09.37 SS.reconfigure :exhale @22:00:09.41 SS.hold @22:00:12.47 washCycleDA #1 of 2 @22:00:12.48 PSR.select :air @22:00:12.49 PSV.dial PSR @22:00:12.97 Intake @15.5psia, vacuum from 10.0 to 13.0psi @22:00:12.99 SS.reconfigure :inhaling 0.87ml/s @22:00:13.03 SS.seek 25ml @22:00:13.87 Pulling 0.5ml of PSR.wash @22:00:13.88 PSR.select :wash @22:00:13.99 PS.reconfigure :default @22:00:14.03 PS.seek 0.5ml @22:00:19.97 delay 5 seconds @22:00:24.99 PSR.select :air @22:00:25.10 PS.seek 2ml @22:00:28.27 Stroke completed -- waiting for pressure to equalize ... @22:00:28.28 SS.reconfigure :exhale @22:00:28.33 SS.hold @22:00:37.98 Intake.select :close @22:00:38.12 SSV.dial :exhaust @22:00:38.87 Exhaust.select :open @22:00:38.98 SS.seek 0ml @22:00:41.28 PTV.dial :puck @22:00:42.03 PSV.dial PTV @22:00:43.26 PBV.dial :waste1 @22:00:43.68 PS.seek 1.5ml @22:00:48.12 Exhaust.select :close @22:00:48.23 SSV.dial CBV @22:00:48.26 CBV.dial SSV @22:00:49.01 Allowing 130s for 25.0ml of remaining 850.0ml @22:00:49.02 Intake.select :open @22:00:49.59 delay 3 seconds @22:00:51.18 Intake @16.3psia, vacuum from 10.0 to 13.0psi @22:00:51.20 SS.reconfigure :inhaling 0.87ml/s @22:00:51.24 SS.seek 25ml @22:00:52.61 PBV.dial between PSV and waste1 @22:00:52.98 PS.seek 0ml @22:01:08.08 Vacuum too high -- waiting up to 40 seconds @22:01:08.09 SS.reconfigure :exhale @22:01:08.13 SS.hold @22:01:09.12 delay 2:00 @22:01:13.01 SS.reconfigure :inhaling 0.78ml/s @22:01:13.05 SS.seek 25ml @22:01:28.97 Stroke completed -- waiting for pressure to equalize ... @22:01:28.99 SS.reconfigure :exhale @22:01:29.03 SS.hold @22:01:40.16 Intake.select :close @22:01:40.30 SSV.dial :exhaust @22:01:41.04 Exhaust.select :open @22:01:41.15 SS.seek 0ml @22:01:49.83 Exhaust.select :close @22:01:49.94 SSV.dial CBV @22:01:49.96 CBV.dial SSV @22:01:50.71 Allowing 130s for 25.0ml of remaining 825.0ml @22:01:50.72 Intake.select :open @22:01:52.88 Intake @16.2psia, vacuum from 10.0 to 13.0psi @22:01:52.90 SS.reconfigure :inhaling 0.78ml/s @22:01:52.94 SS.seek 25ml @22:02:13.33 Vacuum too high -- waiting up to 40 seconds @22:02:13.34 SS.reconfigure :exhale @22:02:13.38 SS.hold @22:02:19.01 Intake @16.3psia, vacuum from 10.0 to 13.0psi @22:02:19.03 SS.reconfigure :inhaling 0.70ml/s @22:02:19.07 SS.seek 25ml @22:02:35.07 Stroke completed -- waiting for pressure to equalize ... @22:02:35.08 SS.reconfigure :exhale @22:02:35.12 SS.hold @22:02:47.78 Intake.select :close @22:02:47.92 SSV.dial :exhaust @22:02:48.66 Exhaust.select :open @22:02:49.28 SS.seek 0ml @22:02:58.05 Exhaust.select :close @22:02:58.16 SSV.dial CBV @22:02:58.18 CBV.dial SSV @22:02:58.95 Allowing 130s for 25.0ml of remaining 800.0ml @22:02:58.96 Intake.select :open @22:03:01.11 Intake @16.2psia, vacuum from 10.0 to 13.0psi @22:03:01.13 SS.reconfigure :inhaling 0.70ml/s @22:03:01.18 SS.seek 25ml @22:03:09.15 recoverPSRreagent08 @22:03:09.16 PTV.dial :puck @22:03:09.17 PBV.dial :waste1 @22:03:09.51 delay 30 seconds @22:03:20.57 Vacuum too high -- waiting up to 40 seconds @22:03:20.59 SS.reconfigure :exhale @22:03:20.63 SS.hold @22:03:25.49 Intake @16.0psia, vacuum from 10.0 to 13.0psi @22:03:25.51 SS.reconfigure :inhaling 0.63ml/s @22:03:25.55 SS.seek 25ml @22:03:39.53 PBV.dial between PSV and waste1 @22:03:39.89 PSR.select :air @22:03:39.91 PSV.dial PSR @22:03:40.66 PS.reconfigure :fast @22:03:40.70 PS.seek 2.5ml @22:03:46.72 Stroke completed -- waiting for pressure to equalize ... @22:03:46.73 SS.reconfigure :exhale @22:03:46.78 SS.hold @22:04:01.13 Intake.select :close @22:04:01.27 SSV.dial :exhaust @22:04:02.04 Exhaust.select :open @22:04:02.15 SS.seek 0ml @22:04:04.62 PSV.dial PTV @22:04:05.39 delay 5 seconds @22:04:10.41 PSV.dial PSR @22:04:11.00 Exhaust.select :close @22:04:11.11 SSV.dial CBV @22:04:11.13 CBV.dial SSV @22:04:11.20 delay 5 seconds @22:04:11.94 Allowing 130s for 25.0ml of remaining 775.0ml @22:04:11.96 Intake.select :open @22:04:14.11 Intake @16.3psia, vacuum from 10.0 to 13.0psi @22:04:14.13 SS.reconfigure :inhaling 0.63ml/s @22:04:14.17 SS.seek 25ml @22:04:16.23 PSV.dial PTV @22:04:17.00 delay 5 seconds @22:04:22.02 PSV.dial :air @22:04:22.78 PS.seek 0ml @22:04:35.54 Vacuum too high -- waiting up to 40 seconds @22:04:35.55 SS.reconfigure :exhale @22:04:35.60 SS.hold @22:04:41.99 Intake @16.5psia, vacuum from 10.0 to 13.0psi @22:04:42.01 SS.reconfigure :inhaling 0.57ml/s @22:04:42.05 SS.seek 25ml @22:04:45.96 washCycleDA #2 of 2 @22:04:45.98 PSR.select :air @22:04:46.00 PSV.dial PSR @22:04:47.36 Pulling 0.5ml of PSR.wash @22:04:47.37 PSR.select :wash @22:04:47.48 PS.reconfigure :default @22:04:47.52 PS.seek 0.5ml @22:04:53.47 delay 5 seconds @22:04:58.48 PSR.select :air @22:04:58.61 PS.seek 2ml @22:04:59.64 Vacuum too high -- waiting up to 40 seconds @22:04:59.65 SS.reconfigure :exhale @22:05:00.19 SS.hold @22:05:06.70 Intake @16.4psia, vacuum from 10.0 to 13.0psi @22:05:06.72 SS.reconfigure :inhaling 0.51ml/s @22:05:06.76 SS.seek 25ml @22:05:13.39 Stroke completed -- waiting for pressure to equalize ... @22:05:13.41 SS.reconfigure :exhale @22:05:13.45 SS.hold @22:05:14.80 PTV.dial :puck @22:05:14.82 PSV.dial PTV @22:05:15.54 PBV.dial :waste1 @22:05:15.96 PS.seek 1.5ml @22:05:21.96 delay 3 seconds @22:05:24.50 Intake.select :close @22:05:24.65 SSV.dial :exhaust @22:05:24.98 PBV.dial between PSV and waste1 @22:05:25.36 PS.seek 0ml @22:05:25.42 Exhaust.select :open @22:05:25.53 SS.seek 0ml @22:05:34.59 Exhaust.select :close @22:05:34.70 SSV.dial CBV @22:05:34.72 CBV.dial SSV @22:05:35.47 Allowing 130s for 25.0ml of remaining 750.0ml @22:05:35.49 Intake.select :open @22:05:37.64 Intake @16.1psia, vacuum from 10.0 to 13.0psi @22:05:37.66 SS.reconfigure :inhaling 0.51ml/s @22:05:37.70 SS.seek 25ml @22:05:41.71 delay 2:00 @22:06:16.10 Vacuum too high -- waiting up to 40 seconds @22:06:16.11 SS.reconfigure :exhale @22:06:16.16 SS.hold @22:06:24.59 SS.reconfigure :inhaling 0.47ml/s @22:06:24.64 SS.seek 25ml @22:06:38.03 Stroke completed -- waiting for pressure to equalize ... @22:06:38.04 SS.reconfigure :exhale @22:06:38.08 SS.hold @22:06:52.43 Intake.select :close @22:06:52.57 SSV.dial :exhaust @22:06:53.31 Exhaust.select :open @22:06:53.42 SS.seek 0ml @22:07:02.75 Exhaust.select :close @22:07:02.86 SSV.dial CBV @22:07:02.89 CBV.dial SSV @22:07:03.63 Allowing 130s for 25.0ml of remaining 725.0ml @22:07:03.64 Intake.select :open @22:07:05.79 Intake @16.1psia, vacuum from 10.0 to 13.0psi @22:07:05.81 SS.reconfigure :inhaling 0.47ml/s @22:07:05.86 SS.seek 25ml @22:07:41.73 recoverPSRreagent08 @22:07:41.74 PTV.dial :puck @22:07:41.76 PBV.dial :waste1 @22:07:42.12 delay 30 seconds @22:07:45.10 Vacuum too high -- waiting up to 40 seconds @22:07:45.11 SS.reconfigure :exhale @22:07:45.16 SS.hold @22:07:54.97 SS.reconfigure :inhaling 0.43ml/s @22:07:55.01 SS.seek 25ml @22:08:12.14 PBV.dial between PSV and waste1 @22:08:12.51 PSR.select :air @22:08:12.53 PSV.dial PSR @22:08:13.29 PS.reconfigure :fast @22:08:13.34 PS.seek 2.5ml @22:08:13.50 Stroke completed -- waiting for pressure to equalize ... @22:08:13.51 SS.reconfigure :exhale @22:08:13.55 SS.hold @22:08:29.93 Intake.select :close @22:08:30.07 SSV.dial :exhaust @22:08:30.81 Exhaust.select :open @22:08:30.92 SS.seek 0ml @22:08:37.15 PSV.dial PTV @22:08:37.91 delay 5 seconds @22:08:39.76 Exhaust.select :close @22:08:39.87 SSV.dial CBV @22:08:39.89 CBV.dial SSV @22:08:40.66 Allowing 130s for 25.0ml of remaining 700.0ml @22:08:40.67 Intake.select :open @22:08:42.82 Intake @16.1psia, vacuum from 10.0 to 13.0psi @22:08:42.84 SS.reconfigure :inhaling 0.43ml/s @22:08:42.88 SS.seek 25ml @22:08:42.93 PSV.dial PSR @22:08:43.68 delay 5 seconds @22:08:48.70 PSV.dial PTV @22:08:49.46 delay 5 seconds @22:08:54.48 PSV.dial :air @22:08:55.36 PS.seek 0ml @22:09:18.49 getPMLReagent08 @22:09:18.50 PRV.dial :air @22:09:18.94 delay 5 seconds @22:09:22.88 Vacuum too high -- waiting up to 40 seconds @22:09:22.89 SS.reconfigure :exhale @22:09:22.94 SS.hold @22:09:23.96 PTV.dial :PRVmixing @22:09:25.27 delay 5 seconds @22:09:30.34 PRV.dial :ab2 @22:09:30.80 PTV.dial :PRVmixing @22:09:30.81 PSV.dial :mixloop @22:09:31.26 PS.reconfigure :default @22:09:31.82 PS.seek 0.3ml @22:09:33.22 Intake @16.2psia, vacuum from 10.0 to 13.0psi @22:09:33.24 SS.reconfigure :inhaling 0.38ml/s @22:09:33.29 SS.seek 25ml @22:09:35.71 delay 2 seconds @22:09:37.73 PRV.dial :air @22:09:38.27 PS.seek 0.55ml @22:09:41.65 addPMLReagent08 @22:09:41.66 PSV.dial :air @22:09:42.12 PS.seek 2ml @22:09:57.77 PSV.dial :PML @22:09:57.93 Stroke completed -- waiting for pressure to equalize ... @22:09:57.94 SS.reconfigure :exhale @22:09:57.99 SS.hold @22:09:58.25 PTV.dial :mixing @22:09:58.99 PBV.dial :waste1 @22:09:59.41 PS.seek 1.85ml @22:10:01.71 PBV.dial between PSV and waste1 @22:10:02.11 PS.seek 0ml @22:10:18.35 Intake.select :close @22:10:18.50 SSV.dial :exhaust @22:10:19.25 Exhaust.select :open @22:10:19.36 SS.seek 0ml @22:10:21.80 delay 2 seconds @22:10:23.82 delay 8:00 @22:10:28.42 Exhaust.select :close @22:10:28.53 SSV.dial CBV @22:10:28.56 CBV.dial SSV @22:10:29.29 Allowing 130s for 25.0ml of remaining 675.0ml @22:10:29.31 Intake.select :open @22:10:31.46 Intake @16.0psia, vacuum from 10.0 to 13.0psi @22:10:31.48 SS.reconfigure :inhaling 0.38ml/s @22:10:31.52 SS.seek 25ml @22:11:16.97 Vacuum too high -- waiting up to 40 seconds @22:11:16.99 SS.reconfigure :exhale @22:11:17.03 SS.hold @22:11:26.82 Intake @15.6psia, vacuum from 10.0 to 13.0psi @22:11:26.84 SS.reconfigure :inhaling 0.34ml/s @22:11:26.88 SS.seek 25ml @22:11:55.06 Stroke completed -- waiting for pressure to equalize ... @22:11:55.07 SS.reconfigure :exhale @22:11:55.12 SS.hold @22:12:17.41 Intake.select :close @22:12:18.08 SSV.dial :exhaust @22:12:18.82 Exhaust.select :open @22:12:18.93 SS.seek 0ml @22:12:27.77 Exhaust.select :close @22:12:27.88 SSV.dial CBV @22:12:27.90 CBV.dial SSV @22:12:28.67 Allowing 130s for 25.0ml of remaining 650.0ml @22:12:28.68 Intake.select :open @22:12:30.84 Intake @16.0psia, vacuum from 10.0 to 13.0psi @22:12:30.85 SS.reconfigure :inhaling 0.34ml/s @22:12:30.90 SS.seek 25ml @22:13:39.59 Vacuum too high -- waiting up to 40 seconds @22:13:39.61 SS.reconfigure :exhale @22:13:39.65 SS.hold @22:13:54.05 Intake @16.0psia, vacuum from 10.0 to 13.0psi @22:13:54.07 SS.reconfigure :inhaling 0.31ml/s @22:13:54.11 SS.seek 25ml @22:14:08.72 Stroke completed -- waiting for pressure to equalize ... @22:14:08.74 SS.reconfigure :exhale @22:14:08.78 SS.hold @22:14:31.19 Intake.select :close @22:14:31.33 SSV.dial :exhaust @22:14:32.07 Exhaust.select :open @22:14:32.18 SS.seek 0ml @22:14:41.33 Exhaust.select :close @22:14:41.44 SSV.dial CBV @22:14:41.46 CBV.dial SSV @22:14:42.21 Allowing 130s for 25.0ml of remaining 625.0ml @22:14:42.22 Intake.select :open @22:14:44.38 Intake @16.1psia, vacuum from 10.0 to 13.0psi @22:14:44.40 SS.reconfigure :inhaling 0.31ml/s @22:14:44.44 SS.seek 25ml @22:15:57.66 Vacuum too high -- waiting up to 40 seconds @22:15:57.67 SS.reconfigure :exhale @22:15:57.71 SS.hold @22:16:16.04 Intake @16.8psia, vacuum from 10.0 to 13.0psi @22:16:16.06 SS.reconfigure :inhaling 0.28ml/s @22:16:16.10 SS.seek 25ml @22:16:36.50 Stroke completed -- waiting for pressure to equalize ... @22:16:36.52 SS.reconfigure :exhale @22:16:36.56 SS.hold @22:16:52.23 SS.reconfigure :default @22:16:52.28 SS.hold @22:16:52.39 Intake.select :close @22:16:53.04 ShallowSampler::Clogged in WCR -- Filtering too Slowly after 400.0ml @22:16:53.05 SSV.dial :exhaust @22:16:53.79 Exhaust.select :open @22:16:53.89 SS.reconfigure :exhale @22:16:53.94 SS.seek 0ml @22:17:03.02 Exhaust.select :close @22:17:03.13 Sampled 400.0ml @22:17:03.15 Drying puck before fixing for 20:00 @22:17:03.16 CBV.dial :waste2b @22:17:03.90 delay 1:00 @22:18:03.92 CSV.dial :air @22:18:04.38 CS.reconfigure :fast @22:18:04.42 CS.seek 10ml @22:18:23.84 recoverPRVreagent08 @22:18:23.85 PTV.dial :mixing @22:18:23.86 PBV.dial :waste1 @22:18:24.23 delay 30 seconds @22:18:28.61 Pumping wcr puck from tube 3 up to 35psi... @22:18:28.62 CBV.dial SSV @22:18:29.93 CBV.dial between CSV and waste2b @22:18:30.88 CSV.dial between air and CTV @22:18:31.21 CS.seek 7.246ml @22:18:38.24 CTV.dial CSV,avoiding: :air @22:18:38.25 CSV.dial CTV @22:18:39.06 CS.reconfigure :pressurize @22:18:39.11 CS.seek 0ml @22:18:54.25 PBV.dial between PSV and waste1 @22:18:54.62 PSV.dial :air @22:18:55.09 PS.reconfigure :fast @22:18:55.14 PS.seek 2.5ml @22:18:57.72 CBV.dial :waste2b @22:18:58.05 CS.reconfigure :default @22:18:58.09 CS.seek 0ml @22:18:59.17 delay 1:00 @22:19:18.85 PSV.dial :mixloop @22:19:19.31 delay 2 seconds @22:19:21.32 PSV.dial :air @22:19:21.79 delay 2 seconds @22:19:23.81 PSV.dial :mixloop @22:19:24.29 delay 2 seconds @22:19:26.30 PSV.dial :air @22:19:26.77 PS.seek 0ml @22:19:49.98 washCycleDA #1 of 3 @22:19:49.99 PSR.select :air @22:19:50.01 PSV.dial PSR @22:19:51.37 Pulling 0.5ml of PSR.wash @22:19:51.38 PSR.select :wash @22:19:51.50 PS.reconfigure :default @22:19:51.54 PS.seek 0.5ml @22:19:57.45 delay 5 seconds @22:19:59.18 CTV.dial :air,avoiding: :intake @22:20:00.52 Fix #1 of 2 (with RNAlater) for 10:00 @22:20:00.54 CSV.dial CSR @22:20:01.01 Pulling 2ml of CSR.RNAlater @22:20:01.02 CSR.select :RNAlater @22:20:01.14 CS.seek 2ml @22:20:02.47 PSR.select :air @22:20:02.58 PS.seek 2ml @22:20:07.10 delay 5 seconds @22:20:12.12 CSR.select :air @22:20:12.23 CS.reconfigure :fast @22:20:12.27 CS.seek 10ml @22:20:18.66 PTV.dial :puck @22:20:19.38 PSV.dial PTV @22:20:20.12 PBV.dial :waste1 @22:20:20.54 PS.seek 1.5ml @22:20:26.54 delay 3 seconds @22:20:29.56 PBV.dial between PSV and waste1 @22:20:29.94 PS.seek 0ml @22:20:31.77 Pumping wcr puck from tube 3 up to 35psi... @22:20:31.78 CBV.dial SSV @22:20:33.09 CBV.dial between CSV and waste2b @22:20:34.04 CSV.dial between air and CTV @22:20:34.64 CS.seek 7.161ml @22:20:41.89 CTV.dial CSV,avoiding: :air @22:20:41.91 CSV.dial CTV @22:20:43.30 CS.reconfigure :pressurize @22:20:43.34 CS.seek 0ml @22:20:46.10 delay 2:00 @22:20:58.18 delay 10:00 @22:22:46.13 recoverPSRreagent08 @22:22:46.14 PTV.dial :puck @22:22:46.15 PBV.dial :waste1 @22:22:46.52 delay 30 seconds @22:23:16.55 PBV.dial between PSV and waste1 @22:23:16.90 PSR.select :air @22:23:16.92 PSV.dial PSR @22:23:17.68 PS.reconfigure :fast @22:23:17.73 PS.seek 2.5ml @22:23:41.44 PSV.dial PTV @22:23:42.21 delay 5 seconds @22:23:47.23 PSV.dial PSR @22:23:47.98 delay 5 seconds @22:23:53.00 PSV.dial PTV @22:23:53.86 delay 5 seconds @22:23:58.88 PSV.dial :air @22:23:59.65 PS.seek 0ml @22:24:22.63 washCycleDA #2 of 3 @22:24:22.64 PSR.select :air @22:24:22.66 PSV.dial PSR @22:24:24.03 Pulling 0.5ml of PSR.wash @22:24:24.04 PSR.select :wash @22:24:24.15 PS.reconfigure :default @22:24:24.19 PS.seek 0.5ml @22:24:30.13 delay 5 seconds @22:24:35.15 PSR.select :air @22:24:35.27 PS.seek 2ml @22:24:51.44 PTV.dial :puck @22:24:51.45 PSV.dial PTV @22:24:52.19 PBV.dial :waste1 @22:24:52.61 PS.seek 1.5ml @22:24:58.47 delay 3 seconds @22:25:01.49 PBV.dial between PSV and waste1 @22:25:01.86 PS.seek 0ml @22:25:18.00 delay 2:00 @22:27:18.03 recoverPSRreagent08 @22:27:18.04 PTV.dial :puck @22:27:18.05 PBV.dial :waste1 @22:27:18.41 delay 30 seconds @22:27:48.44 PBV.dial between PSV and waste1 @22:27:48.78 PSR.select :air @22:27:48.80 PSV.dial PSR @22:27:49.57 PS.reconfigure :fast @22:27:49.61 PS.seek 2.5ml @22:28:13.34 PSV.dial PTV @22:28:14.22 delay 5 seconds @22:28:19.24 PSV.dial PSR @22:28:19.97 delay 5 seconds @22:28:24.99 PSV.dial PTV @22:28:25.74 delay 5 seconds @22:28:30.75 PSV.dial :air @22:28:31.63 PS.seek 0ml @22:28:54.75 washCycleDA #3 of 3 @22:28:54.76 PSR.select :air @22:28:54.78 PSV.dial PSR @22:28:56.15 Pulling 0.5ml of PSR.wash @22:28:56.16 PSR.select :wash @22:28:56.27 PS.reconfigure :default @22:28:56.31 PS.seek 0.5ml @22:29:02.25 delay 5 seconds @22:29:07.27 PSR.select :air @22:29:07.38 PS.seek 2ml @22:29:23.43 PTV.dial :puck @22:29:23.45 PSV.dial PTV @22:29:24.20 PBV.dial :waste1 @22:29:24.63 PS.seek 1.5ml @22:29:30.53 delay 3 seconds @22:29:33.55 PBV.dial between PSV and waste1 @22:29:33.93 PS.seek 0ml @22:29:50.09 delay 2:00 @22:30:58.21 CBV.dial :waste2b @22:30:58.54 CS.reconfigure :default @22:30:58.58 CS.seek 0ml @22:31:03.47 delay 1:00 @22:31:50.12 recoverPSRreagent08 @22:31:50.13 PTV.dial :puck @22:31:50.14 PBV.dial :waste1 @22:31:50.51 delay 30 seconds @22:32:03.49 CTV.dial :air,avoiding: :intake @22:32:04.82 Fix #2 of 2 (with RNAlater) for 10:00 @22:32:04.83 CSV.dial CSR @22:32:05.33 Pulling 2ml of CSR.RNAlater @22:32:05.34 CSR.select :RNAlater @22:32:05.45 CS.seek 2ml @22:32:11.65 delay 5 seconds @22:32:16.67 CSR.select :air @22:32:16.78 CS.reconfigure :fast @22:32:16.83 CS.seek 10ml @22:32:20.53 PBV.dial between PSV and waste1 @22:32:21.37 PSR.select :air @22:32:21.42 PSV.dial PSR @22:32:22.18 PS.reconfigure :fast @22:32:22.22 PS.seek 2.5ml @22:32:36.65 Pumping wcr puck from tube 3 up to 35psi... @22:32:36.67 CBV.dial SSV @22:32:37.97 CBV.dial between CSV and waste2b @22:32:38.92 CSV.dial between air and CTV @22:32:39.52 CS.seek 7.195ml @22:32:45.99 PSV.dial PTV @22:32:46.66 CTV.dial CSV,avoiding: :air @22:32:46.68 CSV.dial CTV @22:32:46.80 delay 5 seconds @22:32:48.05 CS.reconfigure :pressurize @22:32:48.10 CS.seek 0ml @22:32:51.81 PSV.dial PSR @22:32:52.56 delay 5 seconds @22:32:57.58 PSV.dial PTV @22:32:58.45 delay 5 seconds @22:33:02.33 delay 10:00 @22:33:03.47 PSV.dial :air @22:33:04.36 PS.seek 0ml @22:33:27.46 Gate.power :camera,:ON @22:33:27.48 Mixing DA Substrates @22:33:27.49 PSR.select :air @22:33:27.50 PSV.dial PSR @22:33:29.01 Pulling 0.25ml of PSR.sub1 @22:33:29.04 PSR.select :sub1 @22:33:29.23 PS.reconfigure :default @22:33:29.33 PS.seek 0.25ml @22:33:32.81 delay 5 seconds @22:33:37.88 Pulling 0.25ml of PSR.sub2 @22:33:37.89 PSR.select :sub2 @22:33:38.01 PS.seek 0.5ml @22:33:41.43 delay 5 seconds @22:33:46.45 PSR.select :air @22:33:46.56 PS.seek 2ml @22:34:02.74 PTV.dial :puck @22:34:02.75 PSV.dial PTV @22:34:03.51 PBV.dial :waste1 @22:34:03.94 PS.seek 1.5ml @22:34:09.90 delay 3 seconds @22:34:12.92 PBV.dial between PSV and waste1 @22:34:13.30 PS.seek 0ml @22:34:29.46 delay 10 seconds @22:34:39.49 recoverPSRreagent08 @22:34:39.50 PTV.dial :puck @22:34:39.51 PBV.dial :waste1 @22:34:39.87 delay 30 seconds @22:35:09.90 PBV.dial between PSV and waste1 @22:35:10.26 PSR.select :air @22:35:10.28 PSV.dial PSR @22:35:11.04 PS.reconfigure :fast @22:35:11.09 PS.seek 2.5ml @22:35:34.99 PSV.dial PTV @22:35:35.88 delay 5 seconds @22:35:40.90 PSV.dial PSR @22:35:41.65 delay 5 seconds @22:35:46.67 PSV.dial PTV @22:35:47.43 delay 5 seconds @22:35:52.45 PSV.dial :air @22:35:53.22 PS.seek 0ml @22:36:16.68 Additional air clear @22:36:16.69 PSR.select :air @22:36:16.71 PSV.dial PSR @22:36:18.09 Pulling 2ml of PSR.air @22:36:18.10 PSR.select :air @22:36:18.12 PS.reconfigure :default @22:36:18.16 PS.seek 2ml @22:36:39.43 delay 5 seconds @22:36:44.45 PTV.dial :puck @22:36:44.47 PSV.dial PTV @22:36:45.21 PBV.dial :waste1 @22:36:45.64 PS.seek 1.5ml @22:36:51.52 delay 3 seconds @22:36:54.54 PBV.dial between PSV and waste1 @22:36:54.92 delay 1 second @22:36:55.93 PSV.dial :waste1 @22:36:57.00 PS.seek 0ml @22:37:13.20 Processing Puck Heater at 27.9C before imaging PH.coast @22:37:13.32 Imaging da puck in Processing Clamp while storing it to tube 2 @22:37:13.48 SC.reconfigure :tight @22:37:13.53 SC.seek 2 @22:37:17.05 SC.configure :limp @22:37:17.25 Elbow.reconfigure :tight @22:37:17.29 Elbow.seek 2 @22:37:19.04 Forearm.reconfigure :tight @22:37:19.09 Forearm.seek 2 + 450 counts @22:37:20.12 SE.reconfigure :pick @22:37:20.17 SE.seek :beamBreak @22:37:21.42 SE.configure :default @22:37:21.56 Elbow.reconfigure :limp @22:37:21.61 Forearm.reconfigure :loose @22:37:22.16 Forearm.seek 2 @22:37:22.42 Elbow.hold @22:37:22.48 Elbow.reconfigure :tight @22:37:22.57 SE.seek 28.09 Pucks below Top Plate @22:37:23.12 Forearm.force -20 @22:37:23.15 Forearm.seek 2 + 296 counts @22:37:23.42 Forearm.reconfigure :tight @22:37:23.47 Forearm.seek 2 + 450 counts @22:37:23.68 SE.seek 29.09 Pucks below Top Plate @22:37:25.14 Forearm.reconfigure :loose @22:37:25.18 Forearm.seek :retract @22:37:26.08 Elbow.seek PC @22:37:28.60 Elbow.reconfigure :loose @22:37:28.69 Forearm.seek PC @22:37:29.62 Hand.close @22:37:30.25 PC.seek :open @22:37:35.03 Forearm.seek :retract @22:37:36.00 Elbow.reconfigure :tight @22:37:36.04 Elbow.seek 2 @22:37:38.65 Forearm.reconfigure :tight @22:37:38.70 Forearm.seek 2 + 450 counts @22:37:39.67 Hand.open @22:37:40.31 SE.seek 28.74 Pucks below Top Plate @22:37:40.57 SE.reconfigure :seal @22:37:40.62 SE.seek 27.77 Pucks below Top Plate @22:37:41.15 Lights, camera, ACTION! @22:37:41.18 PuckCamera: snap -bin=2,2 -auto /var/log/esp/da23feb0722h350ml.tif @22:38:00.67 Waiting >20 seconds for da to relinquish FlushPuck @22:38:02.82 SX HX9/16 (USB:16): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 18.334 seconds /var/log/esp/da23feb0722h350ml.tif: TIFF Upload Complete @22:38:02.85 PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da23feb0722h350ml15s.tif @22:38:18.47 SX HX9/16 (USB:16): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15 seconds /var/log/esp/midres/da23feb0722h350ml15s.tif: TIFF Upload Complete @22:38:18.49 PuckCamera: snap -bin=2,2 20.0 /var/log/esp/midres/da23feb0722h350ml20s.tif @22:38:39.15 SX HX9/16 (USB:16): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 20 seconds /var/log/esp/midres/da23feb0722h350ml20s.tif: TIFF Upload Complete @22:38:39.17 Gate.power :camera,:OFF @22:38:39.18 DAfans.email "18.334 sec auto-exposure",Subject: "da23feb0722h350ml images!" @22:38:39.43 Elbow.seek 2 @22:38:39.62 Forearm.seek 2 + 450 counts @22:38:39.68 SE.reconfigure :gently @22:38:39.73 SE.seek 28.49 Pucks below Top Plate @22:38:40.42 Elbow.reconfigure :limp @22:38:40.47 Forearm.reconfigure :loose @22:38:40.51 Forearm.seek 2 @22:38:40.80 Elbow.hold @22:38:40.87 Elbow.reconfigure :tight @22:38:40.93 SE.reconfigure :default @22:38:40.97 SE.seek 28.67 Pucks below Top Plate @22:38:41.45 Forearm.force -20 @22:38:41.49 Forearm.seek 2 + 286 counts @22:38:41.77 Forearm.reconfigure :tight @22:38:41.82 Forearm.seek 2 + 450 counts @22:38:42.07 SE.seek :down @22:38:42.81 SE.stop @22:38:43.29 Forearm.reconfigure :loose @22:38:43.34 Forearm.seek :retract @22:38:44.39 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @22:38:44.77 Elbow.seek FlushPuck::Garage @22:38:46.77 Elbow.reconfigure :loose @22:38:46.95 Forearm.seek :garage @22:38:48.02 Hand.close @22:38:48.90 Forearm.seek :retract @22:38:51.02 Elbow.reconfigure :tight @22:38:51.11 Elbow.seek PC @22:38:52.52 Elbow.reconfigure :loose @22:38:52.73 Forearm.reconfigure :tight @22:38:52.83 Forearm.seek PC @22:38:54.30 PC.seek :closed @22:38:59.07 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.62 @22:38:59.22 Hand.open @22:39:00.02 Forearm.reconfigure :loose @22:39:00.10 Forearm.seek :retract @22:39:01.16 PSR.select :air @22:39:01.19 PSV.dial PSR @22:39:01.82 Pulling 0.5ml of PSR.flush @22:39:01.84 PSR.select :flush @22:39:02.01 PS.seek 0.5ml @22:39:08.08 delay 5 seconds @22:39:13.13 PSR.select :air @22:39:13.28 PS.seek 2.5ml @22:39:15.05 upsync @22:39:34.61 delay 1 second @22:39:35.63 PRV.dial :waste2,avoiding: :ab2 @22:39:37.50 PTV.dial :PRVmixing @22:39:38.81 PSV.dial :mixloop @22:39:39.86 PS.seek :empty @22:40:06.23 PSR.select :air @22:40:06.25 PSV.dial PSR @22:40:07.34 Pulling 0.5ml of PSR.flush @22:40:07.35 PSR.select :flush @22:40:07.47 PS.seek 0.5ml @22:40:13.46 delay 5 seconds @22:40:18.48 PSR.select :air @22:40:18.59 PS.seek 2.5ml @22:40:40.05 delay 1 second @22:40:41.07 PBV.dial :waste1 @22:40:41.44 PTV.dial :mixing @22:40:42.19 PSV.dial :mixloop @22:40:43.26 PS.seek :empty @22:41:09.64 PSV.dial PSR @22:41:10.69 PSR.select :flush @22:41:10.80 PS.seek 1ml @22:41:21.86 PSR.select :air @22:41:21.97 PS.seek 2.5ml @22:41:38.08 PSV.dial PTV @22:41:38.95 PTV.dial :puck @22:41:39.69 PBV.dial :waste1 @22:41:39.71 PS.seek 0ml @22:42:06.26 PSV.dial PSR @22:42:07.00 PSR.select :flush @22:42:07.11 PS.seek 0.5ml @22:42:13.04 PSR.select :air @22:42:13.16 PS.seek 2.5ml @22:42:34.42 PSV.dial PTV @22:42:35.17 PBV.dial PSV @22:42:35.74 PS.seek 0ml @22:43:02.32 PSV.dial PBV @22:43:02.37 CBV.dial :waste2b @22:43:02.71 CS.reconfigure :default @22:43:02.76 CS.seek 0ml @22:43:02.82 PS.seek 2.5ml @22:43:08.17 delay 1:00 @22:43:29.17 PSV.dial :waste1 @22:43:29.93 PS.seek 0ml @22:43:56.29 PSV.dial PSR @22:43:56.75 PSR.select :air @22:43:56.77 PS.seek 2.5ml @22:44:08.20 CTV.dial :air,avoiding: :intake @22:44:09.58 Storing wcr puck in Collection Clamp to tube 2 @22:44:09.74 SC.reconfigure :tight @22:44:09.78 SC.seek 2 @22:44:09.88 SC.configure :limp @22:44:10.07 Elbow.reconfigure :tight @22:44:10.12 Elbow.seek 2 @22:44:12.78 Forearm.reconfigure :tight @22:44:12.83 Forearm.seek 2 + 450 counts @22:44:14.32 SE.reconfigure :pick @22:44:14.40 SE.seek :beamBreak @22:44:15.62 SE.configure :default @22:44:15.77 Elbow.reconfigure :limp @22:44:15.82 Forearm.reconfigure :loose @22:44:15.86 Forearm.seek 2 @22:44:16.13 Elbow.hold @22:44:16.20 Elbow.reconfigure :tight @22:44:16.30 SE.seek 29.1 Pucks below Top Plate @22:44:16.86 Forearm.force -20 @22:44:16.90 Forearm.seek 2 + 271 counts @22:44:17.20 Forearm.reconfigure :tight @22:44:17.25 Forearm.seek 2 + 450 counts @22:44:17.56 SE.seek 30.1 Pucks below Top Plate @22:44:18.12 Forearm.reconfigure :loose @22:44:18.17 Forearm.seek :retract @22:44:19.11 Elbow.seek CC @22:44:20.74 Elbow.reconfigure :loose @22:44:20.83 Forearm.seek CC @22:44:21.80 Hand.close @22:44:22.37 CC.seek :open @22:44:23.14 PSV.dial PTV @22:44:24.00 PBV.dial PSV @22:44:24.02 PS.seek 0ml @22:44:27.08 Forearm.seek :retract @22:44:28.04 Elbow.reconfigure :tight @22:44:28.09 Elbow.seek 2 @22:44:29.77 Elbow.reconfigure :limp @22:44:29.82 Forearm.seek 2 @22:44:30.84 Elbow.hold @22:44:30.90 Elbow.reconfigure :tight @22:44:30.95 Hand.open @22:44:31.73 Forearm.force -20 @22:44:31.77 Forearm.seek 2 + 314 counts @22:44:32.17 Forearm.reconfigure :tight @22:44:32.22 Forearm.seek 2 + 450 counts @22:44:32.58 SE.seek :down @22:44:33.22 SE.stop @22:44:33.70 Forearm.reconfigure :loose @22:44:33.75 Forearm.seek :retract @22:44:34.67 Starting finalPurge @22:44:50.39 PSV.dial PBV @22:44:50.85 PS.seek 2.5ml @22:44:53.86
Waiting >20 seconds for psFlush to relinquish FlushPuck @22:45:17.23 PSV.dial :waste1 @22:45:17.97 PS.seek 0ml @22:45:44.29 PSV.dial PSR @22:45:44.76 PSR.select :flush @22:45:44.88 PS.seek 1ml @22:45:55.95 PSR.select :air @22:45:56.06 PS.seek 2.5ml @22:46:12.38 PSV.dial PTV @22:46:13.10 PTV.dial :puck @22:46:13.12 PBV.dial :waste1 @22:46:13.67 PS.seek 0ml @22:46:40.16 PSV.dial PSR @22:46:40.92 PSR.select :flush @22:46:41.03 PS.seek 0.5ml @22:46:47.04 PSR.select :air @22:46:47.15 PS.seek 2.5ml @22:47:08.45 PSV.dial PTV @22:47:09.18 PBV.dial PSV @22:47:09.75 PS.seek 0ml @22:47:36.10 PSV.dial PBV @22:47:36.58 PS.seek 2.5ml @22:48:02.94 PSV.dial :waste1 @22:48:04.14 PS.seek 0ml @22:48:30.51 PSV.dial PSR @22:48:30.98 PSR.select :air @22:48:31.00 PS.seek 2.5ml @22:48:57.38 PSV.dial PTV @22:48:58.12 PBV.dial PSV @22:48:58.14 PS.seek 0ml @22:49:24.51 PSV.dial PBV @22:49:24.98 PS.seek 2.5ml @22:49:51.41 PSV.dial :waste1 @22:49:52.15 PS.seek 0ml @22:50:18.70
Loading FlushPuck into Collection Clamp from Processing Clamp @22:50:18.87 Elbow.seek PC @22:50:21.37 Elbow.reconfigure :loose @22:50:21.46 Forearm.seek PC @22:50:22.44 Hand.close @22:50:22.99 PC.seek :open @22:50:27.71 Forearm.seek :retract @22:50:28.68 Elbow.reconfigure :tight @22:50:28.72 Elbow.seek CC @22:50:32.34 Elbow.reconfigure :loose @22:50:32.43 Forearm.reconfigure :tight @22:50:32.47 Forearm.seek CC @22:50:33.67 CC.seek :closed @22:50:38.18 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.44 @22:50:38.26 Hand.open @22:50:38.93 Forearm.reconfigure :loose @22:50:38.97 Forearm.seek :retract @22:50:39.92 Flushing the Collection Valve::Manifold @22:50:40.04 CSV.dial CSR @22:50:40.53 Pulling 5ml of CSR.flush @22:50:40.54 CSR.select :flush @22:50:40.66 CS.seek 5ml @22:50:54.27 delay 5 seconds @22:50:59.29 CSR.select :air @22:50:59.44 CS.reconfigure :fast @22:50:59.48 CS.seek 7.5ml @22:51:06.26 CSV.dial :waste @22:51:07.53 CS.reconfigure :default @22:51:07.57 CS.seek 0ml @22:51:27.60 Flushing lower manifold's waste1 port @22:51:27.72 CSV.dial :flush @22:51:28.15 CS.reconfigure :fast @22:51:28.20 CS.seek 5ml @22:51:40.79 delay 5 seconds @22:51:45.81 CSV.dial :air @22:51:46.43 CS.seek 7ml @22:51:51.88 CSV.dial CTV @22:51:51.91
CTV.dial CSV @22:51:53.34 CBV.dial :waste1 @22:51:54.09 CS.seek 0ml @22:52:10.63 Flushing lower manifold's waste2 port @22:52:10.75 CSV.dial :flush @22:52:11.47 CS.seek 5ml @22:52:24.04 delay 5 seconds @22:52:29.06 CSV.dial :air @22:52:29.57 CS.seek 7.001ml @22:52:35.07 CSV.dial CTV @22:52:35.09
CTV.dial CSV @22:52:35.54 CBV.dial :waste2 @22:52:36.59 CS.seek 0ml @22:52:53.04 Flushing lower manifold's waste2b port @22:52:53.15 CSV.dial :flush @22:52:53.89 CS.seek 5ml @22:53:06.60 delay 5 seconds @22:53:11.62 CSV.dial :air @22:53:12.13 CS.seek 7ml @22:53:17.70 CSV.dial CTV @22:53:17.74
CTV.dial CSV @22:53:18.15 CBV.dial :waste2b @22:53:19.19 CS.seek 0ml @22:53:35.60 Disinfecting external Intake with kill @22:53:35.71 CSV.dial CSR @22:53:36.20 Pulling 8ml of CSR.kill @22:53:36.21 CSR.select :kill @22:53:36.79 CS.seek 8ml @22:53:56.63 delay 5 seconds @22:54:01.65 CSR.select :air @22:54:01.76 CS.seek 10ml @22:54:07.30 CSV.dial CBV @22:54:07.35
CBV.dial CSV @22:54:08.35 CTV.dial :intake @22:54:09.13 Intake.select :open @22:54:09.24 CS.seek 2ml @22:54:27.97 Intake.select :close @22:54:28.07 CTV.dial :air @22:54:28.82 CS.reconfigure :default @22:54:28.87 CS.seek 0ml @22:54:34.82 CSV.dial CSR @22:54:35.87 Pulling 5ml of CSR.flush @22:54:35.88 CSR.select :flush @22:54:35.99 CS.seek 5ml @22:54:49.79 delay 5 seconds @22:54:54.81 CSR.select :air @22:54:54.92 CS.seek 10ml @22:55:08.47 CSV.dial CBV @22:55:09.59 CS.seek 0ml @22:55:36.16 Garaging FlushPuck in Collection Clamp @22:55:36.23 Elbow.reconfigure :tight @22:55:36.27 Elbow.seek CC @22:55:36.63 Elbow.reconfigure :loose @22:55:36.72 Forearm.seek CC @22:55:37.65 Hand.close @22:55:38.24 CC.seek :open @22:55:42.83 Forearm.seek :retract @22:55:43.78 Elbow.reconfigure :tight @22:55:43.82 Elbow.seek :garage @22:55:46.74 Elbow.reconfigure :loose @22:55:46.83 Forearm.reconfigure :tight @22:55:46.87 Forearm.seek :garage @22:55:47.80 Hand.open @22:55:48.42 Forearm.reconfigure :loose @22:55:48.46 Forearm.seek :retract @22:55:49.41 Completed [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] begun 20:48:26 @22:55:49.45 Asleep after completing DA_WCR_020723b assays @22:55:49.47 GoodNews.email "{1 => 33,\n 2 => 31,\n 3 => 2,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@22:47:51, 17% flow, 17.3C, 34% humidity, 14.7psia, 13.788V, 0.315A, 0.302A avg, 4.34W\nSampled 400.0ml",Subject: "Asleep after completing DA_WCR_020723b assays" @22:55:54.63 upsync @14:43:40.32UTC14-Feb-23
ESP2 v3.41 I2C Gateway v3.20 for core bus @14:43:42.91 Configuration for short puck jake 1/11/21 by brent@mbari.org /home/esp/esp2/type/shallow/jake/configure.rb @14:43:46.89 Polling Can every 10:00 @14:43:47.07 Cleanly exited at 14:41:09UTC14-Feb-23 @14:43:47.11 Loading /home/esp/esp2/mission/DA_WCR_021423.rb mission @14:43:47.16 Preparing to sleep until + 00:00:05 @14:43:47.18 GoodNews.email "Starting DA_WCR_021423 w/pucks in tube 3\n{1 => 33,\n 2 => 31,\n 3 => 2,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@14:43:46, 16% flow, 17.8C, 36% humidity, 14.7psia, 13.788V, 0.248A, 0.230A avg, 3.42W",Subject: "DA_WCR_021423 begun" @14:43:47.22
Asleep til + 00:00:05 @14:43:47.26 Continuing at 14:43 @14:43:47.50 Gate.power :core,:OFF @14:43:47.52 Gate.power :raw,:OFF @14:43:47.53 Shutdown @14:43:51.19 GoodNews.email "Then, in tube 3: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2/mission/DA_WCR_021423.rb:14\n{1 => 33,\n 2 => 31,\n 3 => 2,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@14:43:47, 16% flow, 17.8C, 36% humidity, 14.7psia, 13.788V, 0.248A, 0.230A avg, 3.42W",Subject: "Asleep until 14:43" @14:43:52.13
Powering core bus @14:43:52.95 upsync @14:43:56.41 upsync @14:43:57.49
Gate.power :raw,:ON @14:43:58.35 CS.configure :default @14:43:58.44 PS.configure :default @14:43:58.53 SS.configure :default @14:43:58.62 CC.configure :default @14:43:58.71 PC.configure :default @14:43:58.82 Forearm.configure :loose @14:43:58.90 Elbow.configure :loose @14:43:59.01 SC.configure :loose @14:43:59.10 SE.configure :default @14:43:59.14 CH.coast @14:43:59.19 CH.configure :default @14:43:59.22 PH.coast @14:43:59.27 PH.configure :default @14:43:59.30 All dwarf controllers running firmware version 3.85 @14:43:59.41 Valve.reset @14:43:59.64 Intake.select :close @14:43:59.68 Exhaust.select :close @14:43:59.70 CTV.dial :air @14:43:59.79 PSV.dial :air @14:44:00.00 CSV.dial :waste1 @14:44:00.06
Forearm.reconfigure :loose @14:44:00.18 Forearm.home.seek :retract @14:44:00.57 SSV.dial :spit,avoiding: :exhaust @14:44:00.87 PS.reconfigure :default @14:44:00.94 PS.home.seek 0ml @14:44:00.97 Hand.close @14:44:00.99
Elbow.jog 1500 @14:44:01.39 CS.reconfigure :fast @14:44:01.42 SS.reconfigure :default @14:44:01.51 CS.home.seek 0ml @14:44:01.56 SS.home.seek 0ml @14:44:01.66
Elbow.reconfigure :loose @14:44:01.70 Elbow.home.seek :clear @14:44:06.21 SE.reconfigure :default @14:44:06.25 SE.home.seek :down @14:44:06.85 SC.reconfigure :tight @14:44:06.89 SC.home.seek :initial @14:44:09.32 PS.stop @14:44:35.25
SC.configure :limp @14:44:35.30 Forearm.seek :retract @14:44:35.36 Elbow.seek :hammer @14:44:36.40 Forearm.reconfigure :tight @14:44:36.44 Forearm.seek retracted @14:44:36.69 PC.reconfigure :default @14:44:36.71
CC.reconfigure :default @14:44:36.79 PC.home.seek :open @14:44:36.82
CC.home.seek :open @14:44:40.32 Phase [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] @14:44:40.33 Notice.email "At 14:44:40 began in tube 3: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2/mission/DA_WCR_021423.rb:14\n{1 => 33,\n 2 => 31,\n 3 => 2,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@14:44:40, 17% flow, 17.8C, 36% humidity, 14.7psia, 13.749V, 0.387A, 0.375A avg, 5.32W",Subject: "Started DA_WCR_021423 phase initialPurge; da [1000, 1000] #da and wsr are run simultanelously" @14:44:40.36
Starting initialPurge @14:44:40.38 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:44:40.44 Hand.open @14:44:41.20 Elbow.reconfigure :tight @14:44:41.24 Elbow.seek FlushPuck::Garage @14:44:43.13 Elbow.reconfigure :loose @14:44:43.21 Forearm.reconfigure :loose @14:44:43.26 Forearm.seek :garage @14:44:44.25 Hand.close @14:44:44.98 Forearm.seek :retract @14:44:45.49 upsync @14:44:45.95
Elbow.reconfigure :tight @14:44:45.99 Elbow.seek CC @14:44:48.94 Elbow.reconfigure :loose @14:44:49.03 Forearm.reconfigure :tight @14:44:49.08 Forearm.seek CC @14:44:50.27 CC.seek :closed @14:44:54.80 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.29 @14:44:54.87 Hand.open @14:44:55.51 Forearm.reconfigure :loose @14:44:55.55 Forearm.seek :retract @14:44:56.51 Flushing the Collection Valve::Manifold @14:44:56.63 CSV.dial CSR @14:44:57.95 Pulling 5ml of CSR.flush @14:44:57.96 CSR.select :flush @14:44:58.07 CS.reconfigure :default @14:44:58.11 CS.seek 5ml @14:45:12.12 delay 5 seconds @14:45:17.13 CSR.select :air @14:45:17.28 CS.reconfigure :fast @14:45:17.33 CS.seek 7.501ml @14:45:24.08 CSV.dial :waste @14:45:25.35 CS.reconfigure :default @14:45:25.39 CS.seek 0ml @14:45:45.36 Purging external Intake @14:45:45.48 CSV.dial :flush @14:45:45.91 CS.reconfigure :fast @14:45:45.96 CS.seek 10ml @14:46:10.36 delay 5 seconds @14:46:15.38 CSV.dial CBV @14:46:15.41
CBV.dial CSV @14:46:16.16 CTV.dial :intake @14:46:16.95 Intake.select :open @14:46:17.06 CS.seek 0ml @14:46:40.58 Intake.select :close @14:46:40.68 CTV.dial :air @14:46:41.47 Flushing upper manifold @14:46:41.59 CSV.dial :flush @14:46:42.32 CS.seek 5ml @14:46:55.08 delay 5 seconds @14:47:00.10 CSV.dial :air @14:47:00.59 CS.seek 7ml @14:47:06.05 CSV.dial CBV @14:47:06.07
CBV.dial CSV @14:47:07.10 CTV.dial :air @14:47:07.11 CS.seek 0ml @14:47:23.61 delay 7 seconds @14:47:30.64 CS.seek 3ml @14:47:38.61 CSV.dial :waste @14:47:39.05 CS.seek 0ml @14:47:46.77 CSV.dial :flush @14:47:47.21 CS.seek 3ml @14:47:55.17 delay 5 seconds @14:48:00.19 CSV.dial :air @14:48:00.68 CS.seek 4.999ml @14:48:06.36 CSV.dial :air @14:48:06.38 CS.seek 0ml @14:48:18.45 delay 7 seconds @14:48:25.48 CS.seek 3ml @14:48:33.39 CSV.dial :waste @14:48:34.12 CS.seek 0ml @14:48:41.74 Garaging FlushPuck in Collection Clamp @14:48:41.81 Elbow.reconfigure :tight @14:48:41.85 Elbow.seek CC @14:48:42.18 Elbow.reconfigure :loose @14:48:42.27 Forearm.seek CC @14:48:43.28 Hand.close @14:48:43.95 CC.seek :open @14:48:48.60 Forearm.seek :retract @14:48:49.55 Elbow.reconfigure :tight @14:48:49.60 Elbow.seek :garage @14:48:52.57 Elbow.reconfigure :loose @14:48:52.67 Forearm.reconfigure :tight @14:48:52.71 Forearm.seek :garage @14:48:53.71 Hand.open @14:48:54.81 Forearm.reconfigure :loose @14:48:54.85 Forearm.seek :retract @14:48:55.80 da sampling at most 1000ml, wcr at most 1000ml @14:48:55.84 Loading sh1 puck from tube 3 into Collection Clamp @14:48:55.85 DAfans.email "Started da in tube 3 at 14:48:55UTC14-Feb-23\n{1 => 33,\n 2 => 31,\n 3 => 2,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@14:44:40, 17% flow, 17.8C, 36% humidity, 14.7psia, 13.749V, 0.387A, 0.375A avg, 5.32W",Subject: "da sampling at most 1000ml, wcr at most 1000ml" @14:48:56.10
SC.reconfigure :tight @14:48:56.15 SC.seek 3 @14:48:59.74 SC.configure :limp @14:48:59.96 Elbow.reconfigure :tight @14:49:00.01 Elbow.seek 3 @14:49:01.15 upsync @14:49:01.96
Forearm.reconfigure :tight @14:49:02.00 Forearm.seek 3 + 450 counts @14:49:03.05 SE.reconfigure :pick @14:49:03.11 SE.seek :beamBreak @14:49:06.17 SE.configure :default @14:49:06.32 Elbow.seek 3 @14:49:06.49 Forearm.seek 3 + 300 counts @14:49:06.66 Forearm.reconfigure :loose @14:49:06.70 Hand.close @14:49:07.33 SE.seek :down @14:49:07.52
Forearm.seek :retract @14:49:08.46 Elbow.seek CC @14:49:10.06 Elbow.reconfigure :loose @14:49:10.08 SE.stop @14:49:10.22
Forearm.reconfigure :tight @14:49:10.26 Forearm.seek CC @14:49:11.55 CC.seek :closed @14:49:16.06 Collection Clamp onSpring/offSpring for 14/27 samples, on/off torque 2.18 @14:49:16.07 Hand.open @14:49:16.75 Forearm.reconfigure :loose @14:49:16.79 Forearm.seek :retract @14:49:17.74 collectSample 1000ml w/bubble point of 24psi @14:49:17.75 Priming 7ml @14:49:17.76 Intake.select :close @14:49:17.77 Exhaust.select :close @14:49:17.78 SS.hold @14:49:17.98 Calibrating pressure and temperature sensors @14:49:18.01 CTV.dial :air @14:49:18.02 SSV.dial :spit,avoiding: :exhaust @14:49:18.04 SS.reconfigure :fast @14:49:18.09 SS.seek 5ml @14:49:20.94 SSV.dial CBV @14:49:20.96
CBV.dial SSV @14:49:22.30 delay 3.8 seconds @14:49:26.21 SSV.dial :spit @14:49:27.52 SS.seek 0ml @14:49:30.29 SSV.dial CBV @14:49:31.59 CTV.dial :intake @14:49:32.38 SSV.dial CBV @14:49:32.40
CBV.dial SSV @14:49:32.41 Allowing 40s for 7.0ml of remaining 7.0ml @14:49:32.42 Intake.select :open @14:49:34.58 Intake @15.0psia, vacuum from 9.5 to 12.5psi @14:49:34.59 SS.reconfigure :inhaling 2.20ml/s @14:49:34.63 SS.seek 7ml @14:49:41.28 Stroke completed -- waiting for pressure to equalize ... @14:49:41.29 SS.reconfigure :exhale @14:49:41.33 SS.hold @14:49:42.19 Intake.select :close @14:49:42.32 SSV.dial :exhaust @14:49:43.08 Exhaust.select :open @14:49:43.18 SS.seek 0ml @14:49:47.26 Exhaust.select :close @14:49:47.37 Sampling 1000.0ml Intake.select :close @14:49:47.38 Exhaust.select :close @14:49:47.39 SS.reconfigure :default @14:49:47.43 SS.hold @14:49:47.61 CTV.dial :intake @14:49:47.63 SSV.dial CBV @14:49:47.66
CBV.dial SSV @14:49:48.42 Allowing 130s for 25.0ml of remaining 1000.0ml @14:49:48.43 Intake.select :open @14:49:50.58 Intake @14.8psia, vacuum from 9.3 to 12.3psi @14:49:50.60 SS.reconfigure :inhaling 2.20ml/s @14:49:50.64 SS.seek 25ml @14:50:02.71 Vacuum too high -- waiting up to 40 seconds @14:50:02.72 SS.reconfigure :exhale @14:50:02.76 SS.hold @14:50:03.46 Intake @9.7psia, vacuum from 4.2 to 7.2psi @14:50:03.47 SS.reconfigure :inhaling 1.98ml/s @14:50:03.52 SS.seek 25ml @14:50:07.77 Stroke completed -- waiting for pressure to equalize ... @14:50:07.78 SS.reconfigure :exhale @14:50:07.82 SS.hold @14:50:12.27 Intake.select :close @14:50:12.40 SSV.dial :exhaust @14:50:13.17 Exhaust.select :open @14:50:13.27 SS.seek 0ml @14:50:22.04 Exhaust.select :close @14:50:22.15 SSV.dial CBV @14:50:22.18
CBV.dial SSV @14:50:22.92 Allowing 130s for 25.0ml of remaining 975.0ml @14:50:22.93 Intake.select :open @14:50:25.08 Intake @14.3psia, vacuum from 8.8 to 11.8psi @14:50:25.10 SS.reconfigure :inhaling 1.98ml/s @14:50:25.14 SS.seek 25ml @14:50:39.61 Vacuum too high -- waiting up to 40 seconds @14:50:39.62 SS.reconfigure :exhale @14:50:39.66 SS.hold @14:50:40.29 Intake @9.9psia, vacuum from 4.4 to 7.4psi @14:50:40.30 SS.reconfigure :inhaling 1.79ml/s @14:50:40.34 SS.seek 25ml @14:50:42.96 Stroke completed -- waiting for pressure to equalize ... @14:50:42.97 SS.reconfigure :exhale @14:50:43.01 SS.hold @14:50:47.79 Intake.select :close @14:50:47.92 SSV.dial :exhaust @14:50:48.68 Exhaust.select :open @14:50:48.79 SS.seek 0ml @14:50:57.59 Exhaust.select :close @14:50:57.69 SSV.dial CBV @14:50:57.73
CBV.dial SSV @14:50:58.47 Allowing 130s for 25.0ml of remaining 950.0ml @14:50:58.48 Intake.select :open @14:51:00.63 Intake @14.1psia, vacuum from 8.6 to 11.6psi @14:51:00.64 SS.reconfigure :inhaling 1.79ml/s @14:51:00.69 SS.seek 25ml @14:51:17.99 Stroke completed -- waiting for pressure to equalize ... @14:51:18.00 SS.reconfigure :exhale @14:51:18.05 SS.hold @14:51:24.53 Intake.select :close @14:51:24.67 SSV.dial :exhaust @14:51:25.43 Exhaust.select :open @14:51:25.54 SS.seek 0ml @14:51:34.56 Exhaust.select :close @14:51:34.66 SSV.dial CBV @14:51:34.68
CBV.dial SSV @14:51:35.45 Allowing 130s for 25.0ml of remaining 925.0ml @14:51:35.46 Intake.select :open @14:51:37.61 Intake @14.2psia, vacuum from 8.7 to 11.7psi @14:51:37.63 SS.reconfigure :inhaling 1.79ml/s @14:51:37.67 SS.seek 25ml @14:51:54.60 Stroke completed -- waiting for pressure to equalize ... @14:51:54.61 SS.reconfigure :exhale @14:51:54.65 SS.hold @14:52:01.05 Intake.select :close @14:52:01.20 SSV.dial :exhaust @14:52:01.95 Exhaust.select :open @14:52:02.06 SS.seek 0ml @14:52:11.10 Exhaust.select :close @14:52:11.21 SSV.dial CBV @14:52:11.26
CBV.dial SSV @14:52:11.96 Allowing 130s for 25.0ml of remaining 900.0ml @14:52:11.97 Intake.select :open @14:52:14.13 Intake @14.2psia, vacuum from 8.7 to 11.7psi @14:52:14.14 SS.reconfigure :inhaling 1.79ml/s @14:52:14.19 SS.seek 25ml @14:52:29.05 Vacuum too high -- waiting up to 40 seconds @14:52:29.06 SS.reconfigure :exhale @14:52:29.10 SS.hold @14:52:29.67 Intake @10.4psia, vacuum from 4.9 to 7.9psi @14:52:29.69 SS.reconfigure :inhaling 1.61ml/s @14:52:29.73 SS.seek 25ml @14:52:32.44 Stroke completed -- waiting for pressure to equalize ... @14:52:32.45 SS.reconfigure :exhale @14:52:32.49 SS.hold @14:52:38.03 Intake.select :close @14:52:38.17 SSV.dial :exhaust @14:52:38.91 Exhaust.select :open @14:52:39.02 SS.seek 0ml @14:52:48.09 Exhaust.select :close @14:52:48.19 SSV.dial CBV @14:52:48.21
CBV.dial SSV @14:52:48.96 Allowing 130s for 25.0ml of remaining 875.0ml @14:52:48.97 Intake.select :open @14:52:51.12 Intake @14.3psia, vacuum from 8.8 to 11.8psi @14:52:51.14 SS.reconfigure :inhaling 1.61ml/s @14:52:51.18 SS.seek 25ml @14:53:09.34 Vacuum too high -- waiting up to 40 seconds @14:53:09.35 SS.reconfigure :exhale @14:53:09.39 SS.hold @14:53:09.78 Intake @10.6psia, vacuum from 5.1 to 8.1psi @14:53:09.79 SS.reconfigure :inhaling 1.45ml/s @14:53:09.84 SS.seek 25ml @14:53:10.16 Stroke completed -- waiting for pressure to equalize ... @14:53:10.17 SS.reconfigure :exhale @14:53:10.22 SS.hold @14:53:15.86 Intake.select :close @14:53:16.00 SSV.dial :exhaust @14:53:16.74 Exhaust.select :open @14:53:16.85 SS.seek 0ml @14:53:25.69 Exhaust.select :close @14:53:25.80 SSV.dial CBV @14:53:25.82
CBV.dial SSV @14:53:26.57 Allowing 130s for 25.0ml of remaining 850.0ml @14:53:26.58 Intake.select :open @14:53:28.73 Intake @14.4psia, vacuum from 8.9 to 11.9psi @14:53:28.75 SS.reconfigure :inhaling 1.45ml/s @14:53:28.79 SS.seek 25ml @14:53:49.32 Stroke completed -- waiting for pressure to equalize ... @14:53:49.33 SS.reconfigure :exhale @14:53:49.37 SS.hold @14:53:54.90 Intake.select :close @14:53:55.03 SSV.dial :exhaust @14:53:55.77 Exhaust.select :open @14:53:55.88 SS.seek 0ml @14:54:04.76 Exhaust.select :close @14:54:04.86 SSV.dial CBV @14:54:04.88
CBV.dial SSV @14:54:05.63 Allowing 130s for 25.0ml of remaining 825.0ml @14:54:05.64 Intake.select :open @14:54:07.80 Intake @14.4psia, vacuum from 8.9 to 11.9psi @14:54:07.81 SS.reconfigure :inhaling 1.45ml/s @14:54:07.85 SS.seek 25ml @14:54:28.36 Stroke completed -- waiting for pressure to equalize ... @14:54:28.37 SS.reconfigure :exhale @14:54:28.41 SS.hold @14:54:34.82 Intake.select :close @14:54:34.95 SSV.dial :exhaust @14:54:35.69 Exhaust.select :open @14:54:35.80 SS.seek 0ml @14:54:44.85 Exhaust.select :close @14:54:44.95 SSV.dial CBV @14:54:44.97
CBV.dial SSV @14:54:45.73 Allowing 130s for 25.0ml of remaining 800.0ml @14:54:45.74 Intake.select :open @14:54:47.89 Intake @14.5psia, vacuum from 9.0 to 12.0psi @14:54:47.90 SS.reconfigure :inhaling 1.45ml/s @14:54:47.95 SS.seek 25ml @14:55:07.88 Stroke completed -- waiting for pressure to equalize ... @14:55:07.89 SS.reconfigure :exhale @14:55:07.93 SS.hold @14:55:15.33 Intake.select :close @14:55:15.46 SSV.dial :exhaust @14:55:16.21 Exhaust.select :open @14:55:16.31 SS.seek 0ml @14:55:25.35 Exhaust.select :close @14:55:25.45 SSV.dial CBV @14:55:25.47
CBV.dial SSV @14:55:26.23 Allowing 130s for 25.0ml of remaining 775.0ml @14:55:26.24 Intake.select :open @14:55:28.39 Intake @14.5psia, vacuum from 9.0 to 12.0psi @14:55:28.41 SS.reconfigure :inhaling 1.45ml/s @14:55:28.45 SS.seek 25ml @14:55:47.60 Vacuum too high -- waiting up to 40 seconds @14:55:47.61 SS.reconfigure :exhale @14:55:47.65 SS.hold @14:55:48.14 Intake @11.4psia, vacuum from 5.9 to 8.9psi @14:55:48.15 SS.reconfigure :inhaling 1.30ml/s @14:55:48.20 SS.seek 25ml @14:55:49.28 Stroke completed -- waiting for pressure to equalize ... @14:55:49.29 SS.reconfigure :exhale @14:55:49.34 SS.hold @14:55:55.74 Intake.select :close @14:55:56.37 SSV.dial :exhaust @14:55:57.11 Exhaust.select :open @14:55:57.22 SS.seek 0ml @14:56:06.03 Exhaust.select :close @14:56:06.14 SSV.dial CBV @14:56:06.16
CBV.dial SSV @14:56:06.91 Allowing 130s for 25.0ml of remaining 750.0ml @14:56:06.92 Intake.select :open @14:56:09.08 Intake @14.5psia, vacuum from 9.0 to 12.0psi @14:56:09.10 SS.reconfigure :inhaling 1.30ml/s @14:56:09.14 SS.seek 25ml @14:56:31.39 Stroke completed -- waiting for pressure to equalize ... @14:56:31.40 SS.reconfigure :exhale @14:56:31.44 SS.hold @14:56:37.94 Intake.select :close @14:56:38.07 SSV.dial :exhaust @14:56:38.82 Exhaust.select :open @14:56:38.92 SS.seek 0ml @14:56:48.00 Exhaust.select :close @14:56:48.11 SSV.dial CBV @14:56:48.16
CBV.dial SSV @14:56:48.86 Allowing 130s for 25.0ml of remaining 725.0ml @14:56:48.87 Intake.select :open @14:56:51.03 Intake @14.6psia, vacuum from 9.1 to 12.1psi @14:56:51.05 SS.reconfigure :inhaling 1.30ml/s @14:56:51.09 SS.seek 25ml @14:57:12.64 Stroke completed -- waiting for pressure to equalize ... @14:57:12.65 SS.reconfigure :exhale @14:57:12.69 SS.hold @14:57:20.06 Intake.select :close @14:57:20.20 SSV.dial :exhaust @14:57:20.94 Exhaust.select :open @14:57:21.05 SS.seek 0ml @14:57:30.19 Exhaust.select :close @14:57:30.29 SSV.dial CBV @14:57:30.31
CBV.dial SSV @14:57:31.07 Allowing 130s for 25.0ml of remaining 700.0ml @14:57:31.08 Intake.select :open @14:57:33.23 Intake @14.6psia, vacuum from 9.1 to 12.1psi @14:57:33.29 SS.reconfigure :inhaling 1.30ml/s @14:57:33.34 SS.seek 25ml @14:57:53.85 Vacuum too high -- waiting up to 40 seconds @14:57:53.86 SS.reconfigure :exhale @14:57:53.90 SS.hold @14:57:54.61 Intake @11.8psia, vacuum from 6.3 to 9.3psi @14:57:54.62 SS.reconfigure :inhaling 1.17ml/s @14:57:54.66 SS.seek 25ml @14:57:56.47 Stroke completed -- waiting for pressure to equalize ... @14:57:56.48 SS.reconfigure :exhale @14:57:56.52 SS.hold @14:58:02.91 Intake.select :close @14:58:03.05 SSV.dial :exhaust @14:58:03.78 Exhaust.select :open @14:58:03.89 SS.seek 0ml @14:58:12.69 Exhaust.select :close @14:58:12.79 SSV.dial CBV @14:58:12.81
CBV.dial SSV @14:58:13.57 Allowing 130s for 25.0ml of remaining 675.0ml @14:58:13.58 Intake.select :open @14:58:15.73 Intake @14.5psia, vacuum from 9.0 to 12.0psi @14:58:15.74 SS.reconfigure :inhaling 1.17ml/s @14:58:15.79 SS.seek 25ml @14:58:40.20 Stroke completed -- waiting for pressure to equalize ... @14:58:40.21 SS.reconfigure :exhale @14:58:40.25 SS.hold @14:58:47.61 Intake.select :close @14:58:47.75 SSV.dial :exhaust @14:58:48.49 Exhaust.select :open @14:58:48.59 SS.seek 0ml @14:58:57.47 Exhaust.select :close @14:58:57.57 SSV.dial CBV @14:58:57.59
CBV.dial SSV @14:58:58.35 Allowing 130s for 25.0ml of remaining 650.0ml @14:58:58.36 Intake.select :open @14:59:00.51 Intake @14.6psia, vacuum from 9.1 to 12.1psi @14:59:00.52 SS.reconfigure :inhaling 1.17ml/s @14:59:00.57 SS.seek 25ml @14:59:24.95 Stroke completed -- waiting for pressure to equalize ... @14:59:24.96 SS.reconfigure :exhale @14:59:25.00 SS.hold @14:59:33.55 Intake.select :close @14:59:33.69 SSV.dial :exhaust @14:59:34.42 Exhaust.select :open @14:59:34.53 SS.seek 0ml @14:59:43.31 Exhaust.select :close @14:59:43.42 SSV.dial CBV @14:59:43.47
CBV.dial SSV @14:59:44.17 Allowing 130s for 25.0ml of remaining 625.0ml @14:59:44.18 Intake.select :open @14:59:46.34 Intake @14.6psia, vacuum from 9.1 to 12.1psi @14:59:46.35 SS.reconfigure :inhaling 1.17ml/s @14:59:46.40 SS.seek 25ml @15:00:08.41 Vacuum too high -- waiting up to 40 seconds @15:00:08.42 SS.reconfigure :exhale @15:00:08.46 SS.hold @15:00:10.53 Intake @12.2psia, vacuum from 6.7 to 9.7psi @15:00:10.54 SS.reconfigure :inhaling 1.06ml/s @15:00:10.59 SS.seek 25ml @15:00:13.45 Stroke completed -- waiting for pressure to equalize ... @15:00:13.46 SS.reconfigure :exhale @15:00:13.50 SS.hold @15:00:19.99 Intake.select :close @15:00:20.13 SSV.dial :exhaust @15:00:20.87 Exhaust.select :open @15:00:20.98 SS.seek 0ml @15:00:29.81 Exhaust.select :close @15:00:29.91 SSV.dial CBV @15:00:29.94
CBV.dial SSV @15:00:30.69 Allowing 130s for 25.0ml of remaining 600.0ml @15:00:30.70 Intake.select :open @15:00:32.85 Intake @14.5psia, vacuum from 9.0 to 12.0psi @15:00:32.87 SS.reconfigure :inhaling 1.06ml/s @15:00:32.91 SS.seek 25ml @15:00:59.45 Stroke completed -- waiting for pressure to equalize ... @15:00:59.46 SS.reconfigure :exhale @15:00:59.50 SS.hold @15:01:08.03 Intake.select :close @15:01:08.98 SSV.dial :exhaust @15:01:09.72 Exhaust.select :open @15:01:09.82 SS.seek 0ml @15:01:18.89 Exhaust.select :close @15:01:18.99 SSV.dial CBV @15:01:19.01
CBV.dial SSV @15:01:19.77 Allowing 130s for 25.0ml of remaining 575.0ml @15:01:19.78 Intake.select :open @15:01:21.93 Intake @14.5psia, vacuum from 9.0 to 12.0psi @15:01:21.94 SS.reconfigure :inhaling 1.06ml/s @15:01:21.98 SS.seek 25ml @15:01:47.01 Vacuum too high -- waiting up to 40 seconds @15:01:47.02 SS.reconfigure :exhale @15:01:47.06 SS.hold @15:01:49.11 Intake @12.3psia, vacuum from 6.8 to 9.8psi @15:01:49.13 SS.reconfigure :inhaling 0.95ml/s @15:01:49.17 SS.seek 25ml @15:01:50.70 Stroke completed -- waiting for pressure to equalize ... @15:01:50.71 SS.reconfigure :exhale @15:01:50.75 SS.hold @15:01:57.16 Intake.select :close @15:01:57.30 SSV.dial :exhaust @15:01:58.04 Exhaust.select :open @15:01:58.15 SS.seek 0ml @15:02:07.14 Exhaust.select :close @15:02:07.24 SSV.dial CBV @15:02:07.26
CBV.dial SSV @15:02:08.50 Allowing 130s for 25.0ml of remaining 550.0ml @15:02:08.51 Intake.select :open @15:02:10.66 Intake @14.5psia, vacuum from 9.0 to 12.0psi @15:02:10.68 SS.reconfigure :inhaling 0.95ml/s @15:02:10.72 SS.seek 25ml @15:02:38.66 Vacuum too high -- waiting up to 40 seconds @15:02:38.67 SS.reconfigure :exhale @15:02:38.71 SS.hold @15:02:40.76 Intake @12.4psia, vacuum from 6.9 to 9.9psi @15:02:40.77 SS.reconfigure :inhaling 0.87ml/s @15:02:40.82 SS.seek 25ml @15:02:42.04 Stroke completed -- waiting for pressure to equalize ... @15:02:42.05 SS.reconfigure :exhale @15:02:42.09 SS.hold @15:02:47.71 Intake.select :close @15:02:47.85 SSV.dial :exhaust @15:02:48.59 Exhaust.select :open @15:02:48.70 SS.seek 0ml @15:02:57.82 Exhaust.select :close @15:02:57.93 SSV.dial CBV @15:02:57.95
CBV.dial SSV @15:02:58.70 Allowing 130s for 25.0ml of remaining 525.0ml @15:02:58.71 Intake.select :open @15:03:00.86 Intake @14.5psia, vacuum from 9.0 to 12.0psi @15:03:00.88 SS.reconfigure :inhaling 0.87ml/s @15:03:00.92 SS.seek 25ml @15:03:31.79 Stroke completed -- waiting for pressure to equalize ... @15:03:31.80 SS.reconfigure :exhale @15:03:31.84 SS.hold @15:03:40.29 Intake.select :close @15:03:40.43 SSV.dial :exhaust @15:03:41.17 Exhaust.select :open @15:03:41.27 SS.seek 0ml @15:03:50.10 Exhaust.select :close @15:03:50.20 SSV.dial CBV @15:03:50.22
CBV.dial SSV @15:03:50.98 Allowing 130s for 25.0ml of remaining 500.0ml @15:03:50.99 Intake.select :open @15:03:53.14 Intake @14.5psia, vacuum from 9.0 to 12.0psi @15:03:53.16 SS.reconfigure :inhaling 0.87ml/s @15:03:53.20 SS.seek 25ml @15:04:24.77 Stroke completed -- waiting for pressure to equalize ... @15:04:24.78 SS.reconfigure :exhale @15:04:24.82 SS.hold @15:04:33.30 Intake.select :close @15:04:33.44 SSV.dial :exhaust @15:04:34.18 Exhaust.select :open @15:04:34.29 SS.seek 0ml @15:04:43.35 Exhaust.select :close @15:04:43.45 SSV.dial CBV @15:04:43.48
CBV.dial SSV @15:04:44.23 Allowing 130s for 25.0ml of remaining 475.0ml @15:04:44.24 Intake.select :open @15:04:46.39 Intake @14.2psia, vacuum from 8.7 to 11.7psi @15:04:46.41 SS.reconfigure :inhaling 0.87ml/s @15:04:46.45 SS.seek 25ml @15:05:13.81 Vacuum too high -- waiting up to 40 seconds @15:05:13.82 SS.reconfigure :exhale @15:05:13.86 SS.hold @15:05:18.02 Intake @12.9psia, vacuum from 7.4 to 10.4psi @15:05:18.04 SS.reconfigure :inhaling 0.78ml/s @15:05:18.08 SS.seek 25ml @15:05:22.88 Stroke completed -- waiting for pressure to equalize ... @15:05:22.89 SS.reconfigure :exhale @15:05:22.93 SS.hold @15:05:28.57 Intake.select :close @15:05:28.70 SSV.dial :exhaust @15:05:29.45 Exhaust.select :open @15:05:29.55 SS.seek 0ml @15:05:38.40 Exhaust.select :close @15:05:38.50 SSV.dial CBV @15:05:38.53
CBV.dial SSV @15:05:39.29 Allowing 130s for 25.0ml of remaining 450.0ml @15:05:39.30 Intake.select :open @15:05:41.46 Intake @14.6psia, vacuum from 9.1 to 12.1psi @15:05:41.47 SS.reconfigure :inhaling 0.78ml/s @15:05:41.52 SS.seek 25ml @15:06:16.43 Stroke completed -- waiting for pressure to equalize ... @15:06:16.44 SS.reconfigure :exhale @15:06:16.48 SS.hold @15:06:26.23 Intake.select :close @15:06:26.37 SSV.dial :exhaust @15:06:27.12 Exhaust.select :open @15:06:27.23 SS.seek 0ml @15:06:36.05 Exhaust.select :close @15:06:36.16 SSV.dial CBV @15:06:36.18
CBV.dial SSV @15:06:36.93 Allowing 130s for 25.0ml of remaining 425.0ml @15:06:36.94 Intake.select :open @15:06:39.09 Intake @14.6psia, vacuum from 9.1 to 12.1psi @15:06:39.11 SS.reconfigure :inhaling 0.78ml/s @15:06:39.15 SS.seek 25ml @15:07:12.22 Vacuum too high -- waiting up to 40 seconds @15:07:12.23 SS.reconfigure :exhale @15:07:12.27 SS.hold @15:07:16.46 Intake @13.1psia, vacuum from 7.6 to 10.6psi @15:07:16.48 SS.reconfigure :inhaling 0.70ml/s @15:07:16.52 SS.seek 25ml @15:07:18.79 Stroke completed -- waiting for pressure to equalize ... @15:07:18.80 SS.reconfigure :exhale @15:07:18.84 SS.hold @15:07:25.24 Intake.select :close @15:07:25.38 SSV.dial :exhaust @15:07:26.12 Exhaust.select :open @15:07:26.22 SS.seek 0ml @15:07:35.04 Exhaust.select :close @15:07:35.14 SSV.dial CBV @15:07:35.16
CBV.dial SSV @15:07:35.91 Allowing 130s for 25.0ml of remaining 400.0ml @15:07:35.92 Intake.select :open @15:07:38.08 Intake @14.6psia, vacuum from 9.1 to 12.1psi @15:07:38.10 SS.reconfigure :inhaling 0.70ml/s @15:07:38.14 SS.seek 25ml @15:08:16.02 Stroke completed -- waiting for pressure to equalize ... @15:08:16.03 SS.reconfigure :exhale @15:08:16.07 SS.hold @15:08:27.63 Intake.select :close @15:08:27.77 SSV.dial :exhaust @15:08:28.52 Exhaust.select :open @15:08:28.63 SS.seek 0ml @15:08:37.44 Exhaust.select :close @15:08:37.55 SSV.dial CBV @15:08:37.60
CBV.dial SSV @15:08:38.30 Allowing 130s for 25.0ml of remaining 375.0ml @15:08:38.31 Intake.select :open @15:08:40.47 Intake @14.6psia, vacuum from 9.1 to 12.1psi @15:08:40.49 SS.reconfigure :inhaling 0.70ml/s @15:08:40.53 SS.seek 25ml @15:09:17.68 Vacuum too high -- waiting up to 40 seconds @15:09:17.69 SS.reconfigure :exhale @15:09:17.73 SS.hold @15:09:23.36 Intake @13.4psia, vacuum from 7.9 to 10.9psi @15:09:23.38 SS.reconfigure :inhaling 0.63ml/s @15:09:23.42 SS.seek 25ml @15:09:24.75 Stroke completed -- waiting for pressure to equalize ... @15:09:24.76 SS.reconfigure :exhale @15:09:24.80 SS.hold @15:09:31.32 Intake.select :close @15:09:31.46 SSV.dial :exhaust @15:09:32.21 Exhaust.select :open @15:09:32.31 SS.seek 0ml @15:09:41.11 Exhaust.select :close @15:09:41.22 SSV.dial CBV @15:09:41.27
CBV.dial SSV @15:09:41.97 Allowing 130s for 25.0ml of remaining 350.0ml @15:09:41.98 Intake.select :open @15:09:44.14 Intake @14.6psia, vacuum from 9.1 to 12.1psi @15:09:44.15 SS.reconfigure :inhaling 0.63ml/s @15:09:44.20 SS.seek 25ml @15:10:26.30 Stroke completed -- waiting for pressure to equalize ... @15:10:26.31 SS.reconfigure :exhale @15:10:26.35 SS.hold @15:10:38.89 Intake.select :close @15:10:39.03 SSV.dial :exhaust @15:10:39.77 Exhaust.select :open @15:10:39.87 SS.seek 0ml @15:10:48.91 Exhaust.select :close @15:10:49.01 SSV.dial CBV @15:10:49.03
CBV.dial SSV @15:10:49.78 Allowing 130s for 25.0ml of remaining 325.0ml @15:10:49.79 Intake.select :open @15:10:51.95 Intake @14.6psia, vacuum from 9.1 to 12.1psi @15:10:51.97 SS.reconfigure :inhaling 0.63ml/s @15:10:52.01 SS.seek 25ml @15:11:33.77 Stroke completed -- waiting for pressure to equalize ... @15:11:33.78 SS.reconfigure :exhale @15:11:33.82 SS.hold @15:11:48.06 Intake.select :close @15:11:48.20 SSV.dial :exhaust @15:11:48.94 Exhaust.select :open @15:11:49.51 SS.seek 0ml @15:11:58.34 Exhaust.select :close @15:11:58.45 SSV.dial CBV @15:11:58.47
CBV.dial SSV @15:11:59.22 Allowing 130s for 25.0ml of remaining 300.0ml @15:11:59.23 Intake.select :open @15:12:01.39 Intake @14.5psia, vacuum from 9.0 to 12.0psi @15:12:01.40 SS.reconfigure :inhaling 0.63ml/s @15:12:01.44 SS.seek 25ml @15:12:31.65 Vacuum too high -- waiting up to 40 seconds @15:12:31.66 SS.reconfigure :exhale @15:12:31.70 SS.hold @15:12:36.56 Intake @13.4psia, vacuum from 7.9 to 10.9psi @15:12:36.58 SS.reconfigure :inhaling 0.57ml/s @15:12:36.62 SS.seek 25ml @15:12:50.03 Stroke completed -- waiting for pressure to equalize ... @15:12:50.04 SS.reconfigure :exhale @15:12:50.08 SS.hold @15:13:01.21 Intake.select :close @15:13:01.35 SSV.dial :exhaust @15:13:02.09 Exhaust.select :open @15:13:02.20 SS.seek 0ml @15:13:11.26 Exhaust.select :close @15:13:11.37 SSV.dial CBV @15:13:11.39
CBV.dial SSV @15:13:12.14 Allowing 130s for 25.0ml of remaining 275.0ml @15:13:12.15 Intake.select :open @15:13:14.30 Intake @14.5psia, vacuum from 9.0 to 12.0psi @15:13:14.32 SS.reconfigure :inhaling 0.57ml/s @15:13:14.36 SS.seek 25ml @15:13:55.15 Vacuum too high -- waiting up to 40 seconds @15:13:55.16 SS.reconfigure :exhale @15:13:55.20 SS.hold @15:14:01.60 Intake @13.4psia, vacuum from 7.9 to 10.9psi @15:14:01.61 SS.reconfigure :inhaling 0.51ml/s @15:14:01.65 SS.seek 25ml @15:14:07.64 Stroke completed -- waiting for pressure to equalize ... @15:14:07.65 SS.reconfigure :exhale @15:14:07.69 SS.hold @15:14:17.46 Intake.select :close @15:14:17.64 SSV.dial :exhaust @15:14:18.40 Exhaust.select :open @15:14:18.50 SS.seek 0ml @15:14:27.56 Exhaust.select :close @15:14:27.66 SSV.dial CBV @15:14:27.68
CBV.dial SSV @15:14:28.44 Allowing 130s for 25.0ml of remaining 250.0ml @15:14:28.45 Intake.select :open @15:14:30.60 Intake @14.5psia, vacuum from 9.0 to 12.0psi @15:14:30.61 SS.reconfigure :inhaling 0.51ml/s @15:14:30.66 SS.seek 25ml @15:15:21.50 Stroke completed -- waiting for pressure to equalize ... @15:15:21.51 SS.reconfigure :exhale @15:15:21.55 SS.hold @15:15:36.68 Intake.select :close @15:15:36.81 SSV.dial :exhaust @15:15:37.56 Exhaust.select :open @15:15:37.67 SS.seek 0ml @15:15:46.70 Exhaust.select :close @15:15:46.80 SSV.dial CBV @15:15:46.82
CBV.dial SSV @15:15:47.58 Allowing 130s for 25.0ml of remaining 225.0ml @15:15:47.59 Intake.select :open @15:15:49.74 Intake @14.5psia, vacuum from 9.0 to 12.0psi @15:15:49.76 SS.reconfigure :inhaling 0.51ml/s @15:15:49.80 SS.seek 25ml @15:16:31.49 Vacuum too high -- waiting up to 40 seconds @15:16:31.50 SS.reconfigure :exhale @15:16:31.54 SS.hold @15:16:38.89 Intake @13.7psia, vacuum from 8.2 to 11.2psi @15:16:38.90 SS.reconfigure :inhaling 0.47ml/s @15:16:38.95 SS.seek 25ml @15:16:49.17 Stroke completed -- waiting for pressure to equalize ... @15:16:49.18 SS.reconfigure :exhale @15:16:49.22 SS.hold @15:17:01.88 Intake.select :close @15:17:02.02 SSV.dial :exhaust @15:17:02.76 Exhaust.select :open @15:17:02.87 SS.seek 0ml @15:17:11.90 Exhaust.select :close @15:17:12.00 SSV.dial CBV @15:17:12.03
CBV.dial SSV @15:17:12.78 Allowing 130s for 25.0ml of remaining 200.0ml @15:17:12.79 Intake.select :open @15:17:14.94 Intake @14.5psia, vacuum from 9.0 to 12.0psi @15:17:14.95 SS.reconfigure :inhaling 0.47ml/s @15:17:15.00 SS.seek 25ml @15:18:07.23 Vacuum too high -- waiting up to 40 seconds @15:18:07.24 SS.reconfigure :exhale @15:18:07.29 SS.hold @15:18:15.72 Intake @13.8psia, vacuum from 8.3 to 11.3psi @15:18:15.74 SS.reconfigure :inhaling 0.43ml/s @15:18:15.78 SS.seek 25ml @15:18:19.44 Stroke completed -- waiting for pressure to equalize ... @15:18:19.45 SS.reconfigure :exhale @15:18:19.50 SS.hold @15:18:30.52 Intake.select :close @15:18:30.65 SSV.dial :exhaust @15:18:31.78 Exhaust.select :open @15:18:31.98 SS.seek 0ml @15:18:40.98 Exhaust.select :close @15:18:41.08 SSV.dial CBV @15:18:41.11
CBV.dial SSV @15:18:41.85 Allowing 130s for 25.0ml of remaining 175.0ml @15:18:41.86 Intake.select :open @15:18:44.02 Intake @14.5psia, vacuum from 9.0 to 12.0psi @15:18:44.03 SS.reconfigure :inhaling 0.43ml/s @15:18:44.08 SS.seek 25ml @15:19:46.15 Stroke completed -- waiting for pressure to equalize ... @15:19:46.16 SS.reconfigure :exhale @15:19:46.20 SS.hold @15:20:04.51 Intake.select :close @15:20:04.65 SSV.dial :exhaust @15:20:05.40 Exhaust.select :open @15:20:05.50 SS.seek 0ml @15:20:14.40 Exhaust.select :close @15:20:14.50 SSV.dial CBV @15:20:14.52
CBV.dial SSV @15:20:15.27 Allowing 130s for 25.0ml of remaining 150.0ml @15:20:15.29 Intake.select :open @15:20:17.44 Intake @14.6psia, vacuum from 9.1 to 12.1psi @15:20:17.46 SS.reconfigure :inhaling 0.43ml/s @15:20:17.50 SS.seek 25ml @15:21:05.61 Vacuum too high -- waiting up to 40 seconds @15:21:05.62 SS.reconfigure :exhale @15:21:05.66 SS.hold @15:21:14.10 Intake @13.9psia, vacuum from 8.4 to 11.4psi @15:21:14.12 SS.reconfigure :inhaling 0.38ml/s @15:21:14.16 SS.seek 25ml @15:21:30.41 Stroke completed -- waiting for pressure to equalize ... @15:21:30.42 SS.reconfigure :exhale @15:21:30.46 SS.hold @15:21:44.80 Intake.select :close @15:21:44.94 SSV.dial :exhaust @15:21:45.68 Exhaust.select :open @15:21:45.79 SS.seek 0ml @15:21:54.63 Exhaust.select :close @15:21:54.73 SSV.dial CBV @15:21:54.75
CBV.dial SSV @15:21:55.51 Allowing 130s for 25.0ml of remaining 125.0ml @15:21:55.52 Intake.select :open @15:21:57.67 Intake @14.5psia, vacuum from 9.0 to 12.0psi @15:21:57.68 SS.reconfigure :inhaling 0.38ml/s @15:21:57.73 SS.seek 25ml @15:23:08.55 Stroke completed -- waiting for pressure to equalize ... @15:23:08.56 SS.reconfigure :exhale @15:23:08.60 SS.hold @15:23:31.37 Intake.select :close @15:23:31.51 SSV.dial :exhaust @15:23:32.25 Exhaust.select :open @15:23:32.36 SS.seek 0ml @15:23:41.19 Exhaust.select :close @15:23:41.29 SSV.dial CBV @15:23:41.32
CBV.dial SSV @15:23:42.08 Allowing 130s for 25.0ml of remaining 100.0ml @15:23:42.09 Intake.select :open @15:23:44.26 Intake @14.6psia, vacuum from 9.1 to 12.1psi @15:23:44.27 SS.reconfigure :inhaling 0.38ml/s @15:23:44.32 SS.seek 25ml @15:24:41.10 Vacuum too high -- waiting up to 40 seconds @15:24:41.11 SS.reconfigure :exhale @15:24:41.15 SS.hold @15:24:50.81 Intake @13.9psia, vacuum from 8.4 to 11.4psi @15:24:50.82 SS.reconfigure :inhaling 0.34ml/s @15:24:50.87 SS.seek 25ml @15:25:07.58 Stroke completed -- waiting for pressure to equalize ... @15:25:07.59 SS.reconfigure :exhale @15:25:07.63 SS.hold @15:25:25.86 Intake.select :close @15:25:26.00 SSV.dial :exhaust @15:25:26.73 Exhaust.select :open @15:25:26.84 SS.seek 0ml @15:25:35.69 Exhaust.select :close @15:25:35.79 SSV.dial CBV @15:25:35.81
CBV.dial SSV @15:25:36.56 Allowing 130s for 25.0ml of remaining 75.0ml @15:25:36.57 Intake.select :open @15:25:38.73 Intake @14.6psia, vacuum from 9.1 to 12.1psi @15:25:38.75 SS.reconfigure :inhaling 0.34ml/s @15:25:38.79 SS.seek 25ml @15:27:00.64 Stroke completed -- waiting for pressure to equalize ... @15:27:00.65 SS.reconfigure :exhale @15:27:00.69 SS.hold @15:27:25.12 Intake.select :close @15:27:25.26 SSV.dial :exhaust @15:27:26.00 Exhaust.select :open @15:27:26.10 SS.seek 0ml @15:27:34.95 Exhaust.select :close @15:27:35.05 SSV.dial CBV @15:27:35.07
CBV.dial SSV @15:27:35.83 Allowing 130s for 25.0ml of remaining 50.0ml @15:27:35.84 Intake.select :open @15:27:38.00 Intake @14.6psia, vacuum from 9.1 to 12.1psi @15:27:38.02 SS.reconfigure :inhaling 0.34ml/s @15:27:38.06 SS.seek 25ml @15:28:36.03 Vacuum too high -- waiting up to 40 seconds @15:28:36.04 SS.reconfigure :exhale @15:28:36.08 SS.hold @15:28:46.15 Intake @14.0psia, vacuum from 8.5 to 11.5psi @15:28:46.16 SS.reconfigure :inhaling 0.31ml/s @15:28:46.21 SS.seek 25ml @15:29:13.76 Stroke completed -- waiting for pressure to equalize ... @15:29:13.77 SS.reconfigure :exhale @15:29:13.81 SS.hold @15:29:36.14 Intake.select :close @15:29:36.28 SSV.dial :exhaust @15:29:37.01 Exhaust.select :open @15:29:37.12 SS.seek 0ml @15:29:46.04 Exhaust.select :close @15:29:46.14 SSV.dial CBV @15:29:46.17
CBV.dial SSV @15:29:46.92 Allowing 130s for 25.0ml of remaining 25.0ml @15:29:46.93 Intake.select :open @15:29:49.08 Intake @14.6psia, vacuum from 9.1 to 12.1psi @15:29:49.10 SS.reconfigure :inhaling 0.31ml/s @15:29:49.14 SS.seek 24.99ml @15:31:09.03 Vacuum too high -- waiting up to 40 seconds @15:31:09.04 SS.reconfigure :exhale @15:31:09.08 SS.hold @15:31:23.33 Intake @14.0psia, vacuum from 8.5 to 11.5psi @15:31:23.34 SS.reconfigure :inhaling 0.28ml/s @15:31:23.38 SS.seek 24.99ml @15:31:35.15 Stroke completed -- waiting for pressure to equalize ... @15:31:35.16 SS.reconfigure :exhale @15:31:35.20 SS.hold @15:31:53.42 Intake.select :close @15:31:53.56 SSV.dial :exhaust @15:31:54.31 Exhaust.select :open @15:31:54.41 SS.seek 0ml @15:32:03.27 Exhaust.select :close @15:32:03.38 Sampled 1000.0ml @15:32:03.39 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @15:32:03.40 Intake.select :close @15:32:03.45 CSV.dial :waste @15:32:03.50 CS.seek 0ml @15:32:03.61 SSV.dial CBV,avoiding: :exhaust @15:32:03.63 CSV.dial :air @15:32:03.65
CBV.dial SSV @15:32:04.90 CS.seek 10ml @15:32:06.01
CTV.dial :air @15:32:06.80 delay 15 seconds @15:32:21.82 SSV.dial :spit,avoiding: :exhaust @15:32:23.14 CTV.dial CSV,avoiding: :intake @15:32:23.17
SS.seek 0ml @15:32:23.29 SSV.dial CBV,avoiding: :exhaust @15:32:29.28 CBV.dial between SSV and waste2 @15:32:29.67 CTV.dial CSV,avoiding: :air @15:32:29.68
CSV.dial CTV @15:32:30.14 CS.reconfigure :pressurize @15:32:30.18 CS.seek 0ml @15:32:48.88 BubblePoint: Intake @38.7psia, Outlet @14.5psia @15:32:48.89 CBV.dial SSV @15:32:49.21 SS.reconfigure :default @15:32:49.25 SS.seek 10ml @15:32:54.88 delay 15 seconds @15:33:09.99 Evacuated: Intake @17.0psia, Outlet @8.9psia @15:33:10.01 SSV.dial :spit,avoiding: :exhaust @15:33:11.31 SS.reconfigure :exhale @15:33:11.35 SS.seek 0ml @15:33:16.69 SS.reconfigure :fast @15:33:16.73 SS.seek 5ml @15:33:19.78 SSV.dial CBV,avoiding: :exhaust @15:33:19.80
CBV.dial SSV @15:33:21.12 CTV.dial :air,avoiding: :intake @15:33:22.47 CSV.dial CTV @15:33:22.51
SS.seek 10ml @15:33:23.50 CSV.dial :air @15:33:23.96 CS.reconfigure :fast @15:33:24.00 CS.seek 0ml @15:33:25.15
SSV.dial :spit,avoiding: :exhaust @15:33:26.47 SS.reconfigure :exhale @15:33:26.51 SS.seek 0ml @15:33:31.58 SS.reconfigure :fast @15:33:31.65 SS.seek 5ml @15:33:31.66 CS.stop @15:33:34.50 SSV.dial CBV,avoiding: :exhaust @15:33:34.52
CBV.dial SSV @15:33:36.84 SSV.dial :spit,avoiding: :exhaust @15:33:38.15 SS.reconfigure :default @15:33:38.19 SS.seek 0ml @15:33:41.46 SS.stop @15:33:41.50 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) Intake.select :close @15:33:41.57 CSV.dial :waste @15:33:41.68 SSV.dial CBV,avoiding: :exhaust @15:33:41.70
CBV.dial SSV @15:33:42.34 CS.seek 0ml @15:33:42.41 CSV.dial :air @15:33:43.04
CTV.dial :air @15:33:43.05 delay 15 seconds @15:33:43.14 CS.seek 10ml @15:33:58.08
SSV.dial :spit,avoiding: :exhaust @15:33:59.39 CTV.dial CSV,avoiding: :intake @15:33:59.42
SS.reconfigure :exhale @15:33:59.51 SS.seek 0ml @15:33:59.93 SSV.dial CBV,avoiding: :exhaust @15:34:07.31 CBV.dial between SSV and waste2 @15:34:07.70 CTV.dial CSV,avoiding: :air @15:34:07.71
CSV.dial CTV @15:34:08.29 CS.reconfigure :pressurize @15:34:08.33 CS.seek 0ml @15:34:29.60 BubblePoint: Intake @37.6psia, Outlet @13.6psia CBV.dial SSV @15:34:29.93 SS.reconfigure :default @15:34:29.97 SS.seek 10ml @15:34:35.63 delay 15 seconds @15:34:50.75 Evacuated: Intake @18.5psia, Outlet @10.9psia @15:34:50.76 SSV.dial :spit,avoiding: :exhaust @15:34:52.07 SS.reconfigure :exhale @15:34:52.12 SS.seek 0ml @15:34:57.47 SS.reconfigure :fast @15:34:57.51 SS.seek 5ml @15:35:00.29 SSV.dial CBV,avoiding: :exhaust @15:35:00.31
CBV.dial SSV @15:35:01.63 CTV.dial :air,avoiding: :intake @15:35:02.97 CSV.dial CTV @15:35:03.00
SS.seek 10ml @15:35:04.01 CSV.dial :air @15:35:04.44 CS.reconfigure :fast @15:35:04.49 CS.seek 0ml @15:35:05.54
SSV.dial :spit,avoiding: :exhaust @15:35:06.85 SS.reconfigure :exhale @15:35:06.90 SS.seek 0ml @15:35:09.59 CS.stop @15:35:12.24
SS.reconfigure :fast @15:35:12.35 SS.seek 5ml @15:35:15.16 SSV.dial CBV,avoiding: :exhaust @15:35:15.18
CBV.dial SSV @15:35:17.50 SSV.dial :spit,avoiding: :exhaust @15:35:18.81 SS.reconfigure :default @15:35:18.85 SS.seek 0ml @15:35:22.17 SS.stop @15:35:22.21 Lysing: Adding 2.0ml AqMeOH @15:35:22.22 CBV.dial between CSV and waste1,avoiding: :DBV @15:35:23.71 CTV.dial CSV @15:35:25.03 CSV.dial CSR @15:35:25.82 Pulling 1ml of CSR.AqMeOH @15:35:25.83 CSR.select :AqMeOH @15:35:25.94 CS.reconfigure :default @15:35:25.98 CS.seek 1ml @15:35:29.47 delay 5 seconds @15:35:34.49 CSR.select :air @15:35:34.65 CS.seek 6.001ml @15:35:48.22 delay 2 seconds @15:35:50.23 CSV.dial :waste2 @15:35:50.68 CS.reconfigure :fast @15:35:50.72 CS.seek 0ml @15:36:05.07 CSV.dial CSR @15:36:05.57 Pulling 2ml of CSR.AqMeOH @15:36:05.58 CSR.select :AqMeOH @15:36:05.69 CS.reconfigure :default @15:36:05.73 CS.seek 2ml @15:36:11.91 delay 5 seconds @15:36:16.93 CSR.select :air @15:36:17.08 CS.seek 5ml @15:36:25.78 delay 2 seconds @15:36:27.80 CSV.dial CTV @15:36:28.22 CS.seek 1ml @15:36:39.35 Before lysing Collection Puck Heater at 18.4C/38.2psia @15:36:39.37 Heating Puck.from 3, :sh1 in Collection Clamp to 70C for 10:00 @15:36:39.39 CH.seek 70C,8:00 @15:38:36.65 CH.hold 10:00,70C @15:38:36.83 Begun lysing Collection Puck Heater at 68.1C/45.5psia @15:48:36.85 Ended lysing Collection Puck Heater at 70C/44.1psia @15:48:36.86 CS.seek 0ml @15:48:40.24 CTV.dial between spare and air,avoiding: :intake @15:48:41.27 CSV.dial :air @15:48:41.71 CS.seek 5ml @15:48:55.30 CSV.dial CTV @15:48:55.74 delay 2 seconds @15:48:57.75 CSV.dial :air @15:48:58.21 delay 2 seconds @15:49:00.22 CSV.dial CTV @15:49:01.16 delay 2 seconds @15:49:03.18 CSV.dial :air @15:49:03.63 delay 2 seconds @15:49:05.65 CS.seek 0ml @15:49:19.38 Puck @71.6psia after final lysis pressurization @15:49:19.39 Recovering Lysate into Collection syringe @15:49:19.40 CSV.dial CBV @15:49:20.39 CBV.dial CSV @15:49:20.77 CS.seek 4.999ml @15:49:34.71 delay 5 seconds @15:49:39.72 CTV.dial :air @15:49:40.25 CS.seek 6.999ml @15:49:46.11 delay 5 seconds @15:49:51.13 CSV.dial :air2 @15:49:51.57 CS.seek 10ml @15:50:00.02 CSV.dial between air and CTV,avoiding: CBV @15:50:01.29 Lysing Complete @15:50:01.36 Storing sh1 puck in Collection Clamp to tube 2 @15:50:01.51 SC.reconfigure :tight @15:50:01.56 SC.seek 2 @15:50:05.11 SC.configure :limp @15:50:05.30 Elbow.reconfigure :tight @15:50:05.34 Elbow.seek 2 @15:50:07.08 Forearm.reconfigure :tight @15:50:07.12 Forearm.seek 2 + 450 counts @15:50:08.12 SE.reconfigure :pick @15:50:08.17 SE.seek :beamBreak @15:50:09.25 SE.configure :default @15:50:09.39 Elbow.reconfigure :limp @15:50:09.43 Forearm.reconfigure :loose @15:50:09.47 Forearm.seek 2 @15:50:09.73 Elbow.hold @15:50:09.79 Elbow.reconfigure :tight @15:50:09.88 SE.seek 30.12 Pucks below Top Plate @15:50:10.47 Forearm.force -20 @15:50:10.50 Forearm.seek 2 + 278 counts @15:50:10.77 Forearm.reconfigure :tight @15:50:10.81 Forearm.seek 2 + 450 counts @15:50:11.09 SE.seek 31.12 Pucks below Top Plate @15:50:11.64 Forearm.reconfigure :loose @15:50:11.69 Forearm.seek :retract @15:50:12.61 Elbow.seek CC @15:50:14.29 Elbow.reconfigure :loose @15:50:14.38 Forearm.seek CC @15:50:15.33 Hand.close @15:50:15.89 CC.seek :open @15:50:20.64 Forearm.seek :retract @15:50:21.61 Elbow.reconfigure :tight @15:50:21.65 Elbow.seek 2 @15:50:23.41 Elbow.reconfigure :limp @15:50:23.45 Forearm.seek 2 @15:50:24.44 Elbow.hold @15:50:24.51 Elbow.reconfigure :tight @15:50:24.55 Hand.open @15:50:25.25 Forearm.force -20 @15:50:25.28 Forearm.seek 2 + 290 counts @15:50:25.57 Forearm.reconfigure :tight @15:50:25.62 Forearm.seek 2 + 450 counts @15:50:25.86 SE.seek :down @15:50:26.45 SE.stop @15:50:26.99
Forearm.reconfigure :loose @15:50:27.03 Forearm.seek :retract @15:50:27.99 Tranferring DA @15:50:28.00 Beginning samplePassC @15:50:28.01 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @15:50:28.19 Elbow.seek FlushPuck::Garage @15:50:30.08 Elbow.reconfigure :loose @15:50:30.17 Forearm.seek :garage @15:50:31.10 Hand.close @15:50:31.88 Forearm.seek :retract @15:50:32.85 Elbow.reconfigure :tight @15:50:32.89 Elbow.seek CC @15:50:35.84 Elbow.reconfigure :loose @15:50:36.40 Forearm.reconfigure :tight @15:50:36.44 Forearm.seek CC @15:50:37.68 CC.seek :closed @15:50:42.19 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.38 @15:50:42.26 Hand.open @15:50:42.95 Forearm.reconfigure :loose @15:50:42.99 Forearm.seek :retract @15:50:43.95 PSV.dial PSR @15:50:45.34 Pulling 1.5ml of PSR.flush @15:50:45.35 PSR.select :flush @15:50:45.47 PS.seek 1.5ml @15:51:01.62 delay 5 seconds @15:51:06.64 PSV.dial :waste1 @15:51:07.09 PS.seek 0ml @15:51:23.24 PSV.dial PSR @15:51:23.69 PSR.select :air @15:51:23.80 PS.seek 2.5ml @15:51:50.25 PRV.dial CBV,avoiding: :ab2 @15:51:50.27
CBV.dial PRV @15:51:51.94 PSV.dial :mixloop @15:51:52.98 CTV.dial :air,avoiding: :intake @15:51:52.99 PTV.dial :PRVmixing @15:51:54.31 PS.seek 0ml @15:52:20.68 PSV.dial :air @15:52:21.25 PS.seek 2.5ml @15:52:47.64 PSV.dial :mixloop @15:52:48.09 PS.seek 0ml @15:53:14.46 PSV.dial :air @15:53:14.92 PS.seek 2.5ml @15:53:41.30 PSV.dial :mixloop @15:53:41.76 PS.seek 0ml @15:54:08.15 Garaging FlushPuck in Collection Clamp @15:54:08.22 Elbow.reconfigure :tight @15:54:08.26 Elbow.seek CC @15:54:08.61 Elbow.reconfigure :loose @15:54:08.70 Forearm.seek CC @15:54:09.73 Hand.close @15:54:10.39 CC.seek :open @15:54:15.00 Forearm.seek :retract @15:54:15.95 Elbow.reconfigure :tight @15:54:16.00 Elbow.seek :garage @15:54:18.83 Elbow.reconfigure :loose @15:54:18.92 Forearm.reconfigure :tight @15:54:18.97 Forearm.seek :garage @15:54:19.97 Hand.open @15:54:20.70 Forearm.reconfigure :loose @15:54:20.74 Forearm.seek :retract @15:54:21.68 PSV.dial :mixloop @15:54:21.70 PTV.dial :PRVmixing @15:54:21.71 PRV.dial CBV @15:54:21.73
CBV.dial PRV @15:54:21.75 CTV.dial :air,avoiding: :intake @15:54:21.77 Loading lyfil puck from tube 3 into Collection Clamp @15:54:21.99 SC.reconfigure :tight @15:54:22.03 SC.seek 3 @15:54:25.66 SC.configure :limp @15:54:25.85 Elbow.reconfigure :tight @15:54:25.90 Elbow.seek 3 @15:54:27.80 Forearm.reconfigure :tight @15:54:27.84 Forearm.seek 3 + 450 counts @15:54:28.87 SE.reconfigure :pick @15:54:28.92 SE.seek :beamBreak @15:54:31.96 SE.configure :default @15:54:32.10 Elbow.seek 3 @15:54:32.27 Forearm.seek 3 + 300 counts @15:54:32.61 Forearm.reconfigure :loose @15:54:33.11 Hand.close @15:54:33.78 SE.seek :down @15:54:33.97
Forearm.seek :retract @15:54:34.91 Elbow.seek CC @15:54:36.52 Elbow.reconfigure :loose @15:54:36.59 SE.stop @15:54:36.70
Forearm.reconfigure :tight @15:54:36.74 Forearm.seek CC @15:54:38.04 CC.seek :closed @15:54:42.54 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.08 @15:54:42.55 Hand.open @15:54:43.20 Forearm.reconfigure :loose @15:54:43.25 Forearm.seek :retract @15:54:44.22 CTV.dial CSV,avoiding: :intake @15:54:45.58 CSV.dial CTV @15:54:45.86 PS.seek 2.5ml @15:55:12.23 CS.seek 4.5ml @15:55:27.07 delay 8 seconds @15:55:35.09 PSV.dial :air @15:55:35.55 delay 2 seconds @15:55:37.56 PSV.dial :waste2 @15:55:38.30 PS.seek 0ml @15:56:04.64 CBV.dial between PRV and waste1 @15:56:04.97 CSV.dial :air @15:56:05.42 CS.seek 10ml @15:56:20.35 CSV.dial CTV @15:56:20.80 CS.seek 0ml @15:56:47.16 CBV.dial :waste1 @15:56:47.46 delay 5 seconds @15:56:52.48 CTV.dial :air,avoiding: :intake @15:56:53.88 Storing lyfil puck in Collection Clamp to tube 2 @15:56:54.04 SC.reconfigure :tight @15:56:54.08 SC.seek 2 @15:56:57.68 SC.configure :limp @15:56:57.87 Elbow.reconfigure :tight @15:56:57.91 Elbow.seek 2 @15:56:59.70 Forearm.reconfigure :tight @15:56:59.74 Forearm.seek 2 + 450 counts @15:57:00.76 SE.reconfigure :pick @15:57:00.81 SE.seek :beamBreak @15:57:01.87 SE.configure :default @15:57:02.02 Elbow.reconfigure :limp @15:57:02.06 Forearm.reconfigure :loose @15:57:02.10 Forearm.seek 2 @15:57:02.32 Elbow.hold @15:57:02.38 Elbow.reconfigure :tight @15:57:02.47 SE.seek 31.11 Pucks below Top Plate @15:57:03.00 Forearm.force -20 @15:57:03.03 Forearm.seek 2 + 285 counts @15:57:03.31 Forearm.reconfigure :tight @15:57:03.36 Forearm.seek 2 + 450 counts @15:57:03.55 SE.seek 32.11 Pucks below Top Plate @15:57:04.16 Forearm.reconfigure :loose @15:57:04.20 Forearm.seek :retract @15:57:05.09 Elbow.seek CC @15:57:06.77 Elbow.reconfigure :loose @15:57:06.86 Forearm.seek CC @15:57:07.82 Hand.close @15:57:08.40 CC.seek :open @15:57:13.09 Forearm.seek :retract @15:57:14.59 Elbow.reconfigure :tight @15:57:14.63 Elbow.seek 2 @15:57:16.39 Elbow.reconfigure :limp @15:57:16.44 Forearm.seek 2 @15:57:17.45 Elbow.hold @15:57:17.51 Elbow.reconfigure :tight @15:57:17.56 Hand.open @15:57:18.25 Forearm.force -20 @15:57:18.28 Forearm.seek 2 + 311 counts @15:57:18.57 Forearm.reconfigure :tight @15:57:18.62 Forearm.seek 2 + 450 counts @15:57:18.86 SE.seek :down @15:57:19.38 SE.stop @15:57:19.99
Forearm.reconfigure :loose @15:57:20.04 Forearm.seek :retract @15:57:20.95 PSV.dial :air @15:57:21.69 PS.seek 2.5ml @15:57:48.05 delay 1 second @15:57:49.06 PSV.dial :mixloop @15:57:49.50 delay 1 second @15:57:50.51 PSV.dial :air @15:57:50.97 delay 1 second @15:57:51.98 PSV.dial :mixloop @15:57:52.44 delay 1 second @15:57:53.45 PSV.dial :air @15:57:53.91 delay 1 second @15:57:54.93 PS.seek 0ml @15:58:21.20 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @15:58:21.37 Elbow.seek FlushPuck::Garage @15:58:23.19 Elbow.reconfigure :loose @15:58:23.28 Forearm.seek :garage @15:58:24.31 Hand.close @15:58:25.00 Forearm.seek :retract @15:58:25.99 Elbow.reconfigure :tight @15:58:26.03 Elbow.seek PC @15:58:27.28 Elbow.reconfigure :loose @15:58:27.37 Forearm.reconfigure :tight @15:58:27.42 Forearm.seek PC @15:58:28.67 PC.seek :closed @15:58:33.30 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.55 @15:58:33.36 Hand.open @15:58:34.07 Forearm.reconfigure :loose @15:58:34.11 Forearm.seek :retract @15:58:35.10 PRV.dial :air @15:58:35.54 PSV.dial :mixloop @15:58:36.06 PS.seek 0.3ml @15:58:39.92 PSV.dial PSR @15:58:41.01 Pulling 0.4ml of PSR.air @15:58:41.02 PSR.select :air @15:58:41.04 PS.seek 0.7ml @15:58:45.95 delay 3 seconds @15:58:48.97 PSR.select :air @15:58:48.99 PS.seek 2.5ml @15:59:08.14 delay 3 seconds @15:59:11.16 PSV.dial :mixloop @15:59:12.33 PTV.dial :mixing @15:59:13.09 PBV.dial :waste2 @15:59:14.03 PS.seek 0ml @15:59:40.45 Garaging FlushPuck in Processing Clamp @15:59:40.52 Elbow.reconfigure :tight @15:59:40.56 Elbow.seek PC @15:59:40.68 Elbow.reconfigure :loose @15:59:40.76 Forearm.seek PC @15:59:41.71 Hand.close @15:59:42.26 PC.seek :open @15:59:46.96 Forearm.seek :retract @15:59:47.98 Elbow.reconfigure :tight @15:59:48.02 Elbow.seek :garage @15:59:49.83 Elbow.reconfigure :loose @15:59:49.92 Forearm.reconfigure :tight @15:59:49.96 Forearm.seek :garage @15:59:50.87 Hand.open @15:59:51.57 Forearm.reconfigure :loose @15:59:51.61 Forearm.seek :retract @15:59:52.56 Loading da puck from tube 4 into Processing Clamp @15:59:52.78 SC.reconfigure :tight @15:59:52.82 SC.seek 4 @15:59:59.55 SC.configure :limp @15:59:59.75 Elbow.reconfigure :tight @15:59:59.79 Elbow.seek 4 @16:00:01.65 Forearm.reconfigure :tight @16:00:01.69 Forearm.seek 4 + 450 counts @16:00:02.69 SE.reconfigure :pick @16:00:02.74 SE.seek :beamBreak @16:00:03.65 SE.configure :default @16:00:03.79 Elbow.seek 4 @16:00:03.97 Forearm.seek 4 + 300 counts @16:00:04.41 Forearm.reconfigure :loose @16:00:04.46 Hand.close @16:00:05.03 SE.seek :down @16:00:05.22
Forearm.seek :retract @16:00:05.63 SE.stop @16:00:06.18
Elbow.seek PC @16:00:08.72 Elbow.reconfigure :loose @16:00:08.81 Forearm.reconfigure :tight @16:00:08.85 Forearm.seek PC @16:00:10.08 PC.seek :closed @16:00:14.70 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.61 @16:00:14.71 Hand.open @16:00:15.33 Forearm.reconfigure :loose @16:00:15.37 Forearm.seek :retract @16:00:16.30 Heating Puck.from 4, :da in Processing Clamp to 28C for 2:00:00 @16:00:16.32 PH.seek 28C,5:00 @16:00:51.97 PH.hold 2:00:00,28C @16:00:52.01 delay 1:30 @16:02:22.05 PSR.select :air @16:02:22.06 PSV.dial PSR @16:02:23.16 Pulling 0.5ml of PSR.wash @16:02:23.17 PSR.select :wash @16:02:23.28 PS.seek 0.5ml @16:02:29.17 delay 5 seconds @16:02:34.18 PSR.select :air @16:02:34.30 PS.seek 2ml @16:02:50.51 PTV.dial :puck @16:02:51.23 PSV.dial PTV @16:02:51.99 PBV.dial :waste1 @16:02:53.38 PS.seek 1.5ml @16:02:59.38 delay 3 seconds @16:03:02.40 PBV.dial between PSV and waste1 @16:03:02.77 PS.seek 0ml @16:03:18.92 delay 5:00 @16:08:18.93 recoverPSRreagent08 @16:08:18.94 PTV.dial :puck @16:08:18.96 PBV.dial :waste1 @16:08:19.33 delay 30 seconds @16:08:49.36 PBV.dial between PSV and waste1 @16:08:49.72 PSR.select :air @16:08:49.74 PSV.dial PSR @16:08:50.49 PS.reconfigure :fast @16:08:50.53 PS.seek 2.5ml @16:09:14.41 PSV.dial PTV @16:09:15.63 delay 5 seconds @16:09:20.65 PSV.dial PSR @16:09:21.39 delay 5 seconds @16:09:26.41 PSV.dial PTV @16:09:27.16 delay 5 seconds @16:09:32.18 PSV.dial :air @16:09:33.06 PS.seek 0ml @16:09:56.19 PTV.dial :PRVmixing,via: PRV @16:09:57.50 PSV.dial :mixloop @16:09:57.95 PRV.dial :ab1 @16:09:58.71 PS.reconfigure :slow @16:09:58.75 PS.seek 0.15ml @16:10:02.38 delay 2 seconds @16:10:04.39 CBV.dial PRV @16:10:04.41
PRV.dial CBV @16:10:04.97 PS.seek 0.35ml @16:10:09.50 delay 2 seconds @16:10:11.52 CBV.dial between PRV and PSV @16:10:11.84 PRV.dial :air @16:10:12.27 PS.seek 0.9ml @16:10:23.97 PRV.dial :waste2 @16:10:24.71 PS.seek 0.5ml @16:10:33.34 addPMLReagent08 @16:10:33.35 PSV.dial :air @16:10:33.93 PS.reconfigure :default @16:10:33.97 PS.seek 2ml @16:10:50.15 PSV.dial :PML @16:10:50.59 PTV.dial :mixing @16:10:51.34 PBV.dial :waste1 @16:10:51.76 PS.seek 1.85ml @16:10:54.06 PBV.dial between PSV and waste1 @16:10:54.44 PS.seek 0ml @16:11:14.15 delay 2 seconds @16:11:16.17 Beginning samplePassC @16:11:16.18 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @16:11:16.35 Elbow.reconfigure :tight @16:11:16.39 Elbow.seek FlushPuck::Garage @16:11:17.74 Elbow.reconfigure :loose @16:11:17.83 Forearm.seek :garage @16:11:18.78 Hand.close @16:11:19.50 Forearm.seek :retract @16:11:20.46 Elbow.reconfigure :tight @16:11:20.50 Elbow.seek CC @16:11:23.41 Elbow.reconfigure :loose @16:11:23.51 Forearm.reconfigure :tight @16:11:23.56 Forearm.seek CC @16:11:24.80 CC.seek :closed @16:11:29.35 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.41 @16:11:29.43 Hand.open @16:11:30.07 Forearm.reconfigure :loose @16:11:30.11 Forearm.seek :retract @16:11:31.09 PSV.dial PSR @16:11:32.19 Pulling 1.5ml of PSR.flush @16:11:32.20 PSR.select :flush @16:11:32.32 PS.seek 1.5ml @16:11:48.44 delay 5 seconds @16:11:53.46 PSV.dial :waste1 @16:11:53.91 PS.seek 0ml @16:12:10.06 PSV.dial PSR @16:12:10.51 PSR.select :air @16:12:10.62 PS.seek 2.5ml @16:12:37.19 PRV.dial CBV,avoiding: :ab2 @16:12:37.21
CBV.dial PRV @16:12:38.83 PSV.dial :mixloop @16:12:39.98 CTV.dial :air,avoiding: :intake @16:12:39.99 PTV.dial :PRVmixing @16:12:40.75 PS.seek 0ml @16:13:07.03 PSV.dial :air @16:13:07.49 PS.seek 2.5ml @16:13:33.90 PSV.dial :mixloop @16:13:34.36 PS.seek 0ml @16:14:00.77 PSV.dial :air @16:14:01.70 PS.seek 2.5ml @16:14:28.21 PSV.dial :mixloop @16:14:28.67 PS.seek 0ml @16:14:55.00 Garaging FlushPuck in Collection Clamp @16:14:55.07 Elbow.reconfigure :tight @16:14:55.11 Elbow.seek CC @16:14:55.54 Elbow.reconfigure :loose @16:14:55.63 Forearm.seek CC @16:14:56.57 Hand.close @16:14:57.13 CC.seek :open @16:15:01.73 Forearm.seek :retract @16:15:02.69 Elbow.reconfigure :tight @16:15:02.73 Elbow.seek :garage @16:15:05.57 Elbow.reconfigure :loose @16:15:05.66 Forearm.reconfigure :tight @16:15:05.70 Forearm.seek :garage @16:15:06.73 Hand.open @16:15:07.43 Forearm.reconfigure :loose @16:15:07.48 Forearm.seek :retract @16:15:08.47 delayUntil "16:19:16.16" @16:15:08.50 Whole Cell Archive sampling 1000ml @16:15:08.51 Loading wcr puck from tube 4 into Collection Clamp @16:15:08.74 SC.reconfigure :tight @16:15:08.78 SC.seek 4 @16:15:08.87 SC.configure :limp @16:15:09.07 Elbow.reconfigure :tight @16:15:09.11 Elbow.seek 4 @16:15:11.05 Forearm.reconfigure :tight @16:15:11.10 Forearm.seek 4 + 450 counts @16:15:12.13 SE.reconfigure :pick @16:15:12.18 SE.seek :beamBreak @16:15:13.28 SE.configure :default @16:15:13.43 Elbow.seek 4 @16:15:13.61 Forearm.seek 4 + 300 counts @16:15:13.79 Forearm.reconfigure :loose @16:15:13.84 Hand.close @16:15:14.51 SE.seek :down @16:15:14.70 Forearm.seek :retract @16:15:15.43 SE.stop @16:15:15.64 Elbow.seek CC @16:15:17.36 Elbow.reconfigure :loose @16:15:17.46 Forearm.reconfigure :tight @16:15:17.50 Forearm.seek CC @16:15:18.71 CC.seek :closed @16:15:23.23 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.13 @16:15:23.24 Hand.open @16:15:23.93 Forearm.reconfigure :loose @16:15:23.98 Forearm.seek :retract @16:15:24.97 collectSample 1000ml w/bubble point of 35psi @16:15:24.98 Priming 7ml @16:15:24.99 Intake.select :close @16:15:25.00 Exhaust.select :close @16:15:25.01 SS.hold @16:15:25.21 Calibrating pressure sensors @16:15:25.22 CTV.dial :air @16:15:25.23 SSV.dial :spit,avoiding: :exhaust @16:15:25.25 SS.reconfigure :fast @16:15:25.29 SS.seek 5ml @16:15:28.12 SSV.dial CBV @16:15:28.15 CBV.dial SSV @16:15:29.47 delay 3.8 seconds @16:15:33.87 SSV.dial :spit @16:15:35.18 SS.seek 0ml @16:15:38.05 SSV.dial CBV @16:15:39.38 CTV.dial :intake @16:15:40.15 SSV.dial CBV @16:15:40.17 CBV.dial SSV @16:15:40.20 Allowing 40s for 7.0ml of remaining 7.0ml @16:15:40.21 Intake.select :open @16:15:42.36 Intake @15.1psia, vacuum from 9.6 to 12.6psi @16:15:42.38 SS.reconfigure :inhaling 2.20ml/s @16:15:42.43 SS.seek 7ml @16:15:48.81 Stroke completed -- waiting for pressure to equalize ... @16:15:48.83 SS.reconfigure :exhale @16:15:48.87 SS.hold @16:15:56.31 Intake.select :close @16:15:56.45 SSV.dial :exhaust @16:15:57.21 Exhaust.select :open @16:15:57.32 SS.seek 0ml @16:16:01.20 Exhaust.select :close @16:16:01.31 Sampling 1000.0ml @16:16:01.32 Intake.select :close @16:16:01.33 Exhaust.select :close @16:16:01.34 SS.reconfigure :default @16:16:01.38 SS.hold @16:16:01.58 CTV.dial :intake @16:16:01.60 SSV.dial CBV @16:16:01.62 CBV.dial SSV @16:16:02.39 Allowing 130s for 25.0ml of remaining 1000.0ml @16:16:02.41 Intake.select :open @16:16:04.56 Intake @15.3psia, vacuum from 9.8 to 12.8psi @16:16:04.58 SS.reconfigure :inhaling 2.20ml/s @16:16:04.62 SS.seek 25ml @16:16:12.10 Vacuum too high -- waiting up to 40 seconds @16:16:12.12 SS.reconfigure :exhale @16:16:12.16 SS.hold @16:16:12.87 Intake @12.4psia, vacuum from 6.9 to 9.9psi @16:16:12.89 SS.reconfigure :inhaling 1.98ml/s @16:16:12.93 SS.seek 25ml @16:16:17.11 Vacuum too high -- waiting up to 40 seconds @16:16:17.12 SS.reconfigure :exhale @16:16:17.17 SS.hold @16:16:22.26 Intake @12.5psia, vacuum from 7.0 to 10.0psi @16:16:22.28 SS.reconfigure :inhaling 1.79ml/s @16:16:22.32 SS.seek 25ml @16:16:28.37 Stroke completed -- waiting for pressure to equalize ... @16:16:28.39 SS.reconfigure :exhale @16:16:28.43 SS.hold @16:16:33.34 Intake.select :close @16:16:33.49 SSV.dial :exhaust @16:16:34.25 Exhaust.select :open @16:16:34.36 SS.seek 0ml @16:16:43.20 Exhaust.select :close @16:16:43.31 SSV.dial CBV @16:16:43.33 CBV.dial SSV @16:16:44.10 Allowing 130s for 25.0ml of remaining 975.0ml @16:16:44.11 Intake.select :open @16:16:46.26 Intake @14.7psia, vacuum from 9.2 to 12.2psi @16:16:46.28 SS.reconfigure :inhaling 1.79ml/s @16:16:46.32 SS.seek 25ml @16:16:57.92 Vacuum too high -- waiting up to 40 seconds @16:16:57.93 SS.reconfigure :exhale @16:16:57.97 SS.hold @16:16:58.54 Intake @11.5psia, vacuum from 6.0 to 9.0psi @16:16:59.04 SS.reconfigure :inhaling 1.61ml/s @16:16:59.09 SS.seek 25ml @16:17:04.38 Vacuum too high -- waiting up to 40 seconds @16:17:04.40 SS.reconfigure :exhale @16:17:04.44 SS.hold @16:17:11.09 Intake @13.0psia, vacuum from 7.5 to 10.5psi @16:17:11.11 SS.reconfigure :inhaling 1.45ml/s @16:17:11.15 SS.seek 25ml @16:17:13.80 Stroke completed -- waiting for pressure to equalize ... @16:17:13.81 SS.reconfigure :exhale @16:17:13.85 SS.hold @16:17:15.05 Intake.select :close @16:17:15.19 SSV.dial :exhaust @16:17:15.94 Exhaust.select :open @16:17:16.05 SS.seek 0ml @16:17:24.92 Exhaust.select :close @16:17:25.03 SSV.dial CBV @16:17:25.05 CBV.dial SSV @16:17:25.82 Allowing 130s for 25.0ml of remaining 950.0ml @16:17:25.83 Intake.select :open @16:17:27.98 Intake @14.6psia, vacuum from 9.1 to 12.1psi @16:17:28.00 SS.reconfigure :inhaling 1.45ml/s @16:17:28.04 SS.seek 25ml @16:17:44.91 Vacuum too high -- waiting up to 40 seconds @16:17:44.93 SS.reconfigure :exhale @16:17:44.97 SS.hold @16:17:46.04 Intake @11.7psia, vacuum from 6.2 to 9.2psi @16:17:46.06 SS.reconfigure :inhaling 1.30ml/s @16:17:46.10 SS.seek 25ml @16:17:50.07 Stroke completed -- waiting for pressure to equalize ... @16:17:50.08 SS.reconfigure :exhale @16:17:50.12 SS.hold @16:17:56.52 Intake.select :close @16:17:56.66 SSV.dial :exhaust @16:17:57.41 Exhaust.select :open @16:17:57.52 SS.seek 0ml @16:18:06.34 Exhaust.select :close @16:18:06.45 SSV.dial CBV @16:18:06.47 CBV.dial SSV @16:18:07.24 Allowing 130s for 25.0ml of remaining 925.0ml @16:18:07.25 Intake.select :open @16:18:09.40 Intake @14.7psia, vacuum from 9.2 to 12.2psi @16:18:09.42 SS.reconfigure :inhaling 1.30ml/s @16:18:09.46 SS.seek 25ml @16:18:28.10 Vacuum too high -- waiting up to 40 seconds @16:18:28.11 SS.reconfigure :exhale @16:18:28.15 SS.hold @16:18:28.86 Intake @11.8psia, vacuum from 6.3 to 9.3psi @16:18:28.88 SS.reconfigure :inhaling 1.17ml/s @16:18:28.93 SS.seek 25ml @16:18:33.27 Stroke completed -- waiting for pressure to equalize ... @16:18:33.28 SS.reconfigure :exhale @16:18:33.32 SS.hold @16:18:39.74 Intake.select :close @16:18:39.88 SSV.dial :exhaust @16:18:40.65 Exhaust.select :open @16:18:40.76 SS.seek 0ml @16:18:49.59 Exhaust.select :close @16:18:49.70 SSV.dial CBV @16:18:49.72 CBV.dial SSV @16:18:50.50 Allowing 130s for 25.0ml of remaining 900.0ml @16:18:50.51 Intake.select :open @16:18:52.67 Intake @14.6psia, vacuum from 9.1 to 12.1psi @16:18:52.69 SS.reconfigure :inhaling 1.17ml/s @16:18:52.73 SS.seek 25ml @16:19:16.17 recoverPRVreagent08 @16:19:16.18 PTV.dial :mixing @16:19:16.48 Stroke completed -- waiting for pressure to equalize ... @16:19:16.49 SS.reconfigure :exhale @16:19:16.54 SS.hold @16:19:16.93 PBV.dial :waste1 @16:19:17.33 delay 30 seconds @16:19:23.99 Intake.select :close @16:19:24.13 SSV.dial :exhaust @16:19:24.89 Exhaust.select :open @16:19:25.00 SS.seek 0ml @16:19:34.06 Exhaust.select :close @16:19:34.17 SSV.dial CBV @16:19:34.21 CBV.dial SSV @16:19:34.95 Allowing 130s for 25.0ml of remaining 875.0ml @16:19:34.97 Intake.select :open @16:19:37.12 Intake @14.5psia, vacuum from 9.0 to 12.0psi @16:19:37.14 SS.reconfigure :inhaling 1.17ml/s @16:19:37.18 SS.seek 25ml @16:19:47.35 PBV.dial between PSV and waste1 @16:19:47.72 PSV.dial :air @16:19:48.20 PS.reconfigure :fast @16:19:48.24 PS.seek 2.5ml @16:19:57.26 Vacuum too high -- waiting up to 40 seconds @16:19:57.27 SS.reconfigure :exhale @16:19:57.31 SS.hold @16:19:59.36 Intake @12.1psia, vacuum from 6.6 to 9.6psi @16:19:59.38 SS.reconfigure :inhaling 1.06ml/s @16:19:59.42 SS.seek 25ml @16:20:03.75 Stroke completed -- waiting for pressure to equalize ... @16:20:03.76 SS.reconfigure :exhale @16:20:03.80 SS.hold @16:20:10.23 Intake.select :close @16:20:10.37 SSV.dial :exhaust @16:20:11.14 Exhaust.select :open @16:20:11.25 SS.seek 0ml @16:20:11.99 PSV.dial :mixloop @16:20:12.46 delay 2 seconds @16:20:14.47 PSV.dial :air @16:20:14.93 delay 2 seconds @16:20:16.94 PSV.dial :mixloop @16:20:17.41 delay 2 seconds @16:20:19.43 PSV.dial :air @16:20:19.91 PS.seek 0ml @16:20:20.29 Exhaust.select :close @16:20:20.40 SSV.dial CBV @16:20:20.42 CBV.dial SSV @16:20:21.20 Allowing 130s for 25.0ml of remaining 850.0ml @16:20:21.22 Intake.select :open @16:20:23.37 Intake @14.6psia, vacuum from 9.1 to 12.1psi @16:20:23.39 SS.reconfigure :inhaling 1.06ml/s @16:20:23.43 SS.seek 25ml @16:20:43.43 washCycleDA #1 of 2 @16:20:43.44 PSR.select :air @16:20:43.45 PSV.dial PSR @16:20:44.82 Pulling 0.5ml of PSR.wash @16:20:44.83 PSR.select :wash @16:20:44.94 PS.reconfigure :default @16:20:44.99 PS.seek 0.5ml @16:20:47.53 Vacuum too high -- waiting up to 40 seconds @16:20:47.55 SS.reconfigure :exhale @16:20:47.59 SS.hold @16:20:49.71 Intake @12.3psia, vacuum from 6.8 to 9.8psi @16:20:49.73 SS.reconfigure :inhaling 0.95ml/s @16:20:49.77 SS.seek 25ml @16:20:50.90 delay 5 seconds @16:20:52.56 Stroke completed -- waiting for pressure to equalize ... @16:20:52.57 SS.reconfigure :exhale @16:20:52.61 SS.hold @16:20:55.91 PSR.select :air @16:20:56.07 PS.seek 2ml @16:20:58.20 Intake.select :close @16:20:58.35 SSV.dial :exhaust @16:20:59.11 Exhaust.select :open @16:20:59.22 SS.seek 0ml @16:21:08.37 Exhaust.select :close @16:21:08.48 SSV.dial CBV @16:21:08.50 CBV.dial SSV @16:21:09.28 Allowing 130s for 25.0ml of remaining 825.0ml @16:21:09.29 Intake.select :open @16:21:11.45 Intake @14.7psia, vacuum from 9.2 to 12.2psi @16:21:11.47 SS.reconfigure :inhaling 0.95ml/s @16:21:11.51 SS.seek 25ml @16:21:12.20 PTV.dial :puck @16:21:12.96 PSV.dial PTV @16:21:13.71 PBV.dial :waste1 @16:21:14.14 PS.seek 1.5ml @16:21:20.02 delay 3 seconds @16:21:23.04 PBV.dial between PSV and waste1 @16:21:23.42 PS.seek 0ml @16:21:39.75 Stroke completed -- waiting for pressure to equalize ... @16:21:39.77 delay 2:00 @16:21:39.78 SS.reconfigure :exhale @16:21:39.84 SS.hold @16:21:48.39 Intake.select :close @16:21:48.53 SSV.dial :exhaust @16:21:49.29 Exhaust.select :open @16:21:49.40 SS.seek 0ml @16:21:58.50 Exhaust.select :close @16:21:58.61 SSV.dial CBV @16:21:58.63 CBV.dial SSV @16:21:59.39 Allowing 130s for 25.0ml of remaining 800.0ml @16:21:59.40 Intake.select :open @16:22:01.55 Intake @14.8psia, vacuum from 9.3 to 12.3psi @16:22:01.57 SS.reconfigure :inhaling 0.95ml/s @16:22:01.62 SS.seek 25ml @16:22:29.90 Stroke completed -- waiting for pressure to equalize ... @16:22:29.91 SS.reconfigure :exhale @16:22:29.96 SS.hold @16:22:39.68 Intake.select :close @16:22:39.82 SSV.dial :exhaust @16:22:40.57 Exhaust.select :open @16:22:40.68 SS.seek 0ml @16:22:49.77 Exhaust.select :close @16:22:49.88 SSV.dial CBV @16:22:49.90 CBV.dial SSV @16:22:51.15 Allowing 130s for 25.0ml of remaining 775.0ml @16:22:51.16 Intake.select :open @16:22:53.32 Intake @14.8psia, vacuum from 9.3 to 12.3psi @16:22:53.34 SS.reconfigure :inhaling 0.95ml/s @16:22:53.38 SS.seek 25ml @16:23:16.63 Vacuum too high -- waiting up to 40 seconds @16:23:16.64 SS.reconfigure :exhale @16:23:16.68 SS.hold @16:23:20.24 Intake @12.8psia, vacuum from 7.3 to 10.3psi @16:23:20.26 SS.reconfigure :inhaling 0.87ml/s @16:23:20.30 SS.seek 25ml @16:23:26.29 Stroke completed -- waiting for pressure to equalize ... @16:23:26.30 SS.reconfigure :exhale @16:23:26.34 SS.hold @16:23:32.75 Intake.select :close @16:23:32.89 SSV.dial :exhaust @16:23:33.66 Exhaust.select :open @16:23:33.77 SS.seek 0ml @16:23:39.79 recoverPSRreagent08 @16:23:39.80 PTV.dial :puck @16:23:39.81 PBV.dial :waste1 @16:23:40.17 delay 30 seconds @16:23:42.57 Exhaust.select :close @16:23:42.68 SSV.dial CBV @16:23:42.70 CBV.dial SSV @16:23:43.48 Allowing 130s for 25.0ml of remaining 750.0ml @16:23:43.49 Intake.select :open @16:23:45.65 Intake @14.9psia, vacuum from 9.4 to 12.4psi @16:23:45.67 SS.reconfigure :inhaling 0.87ml/s @16:23:45.71 SS.seek 25ml @16:24:10.19 PBV.dial between PSV and waste1 @16:24:10.55 PSR.select :air @16:24:10.57 PSV.dial PSR @16:24:11.33 PS.reconfigure :fast @16:24:11.38 PS.seek 2.5ml @16:24:15.22 Vacuum too high -- waiting up to 40 seconds @16:24:15.24 SS.reconfigure :exhale @16:24:15.28 SS.hold @16:24:18.97 Intake @13.1psia, vacuum from 7.6 to 10.6psi @16:24:18.99 SS.reconfigure :inhaling 0.78ml/s @16:24:19.03 SS.seek 25ml @16:24:21.83 Stroke completed -- waiting for pressure to equalize ... @16:24:21.85 SS.reconfigure :exhale @16:24:21.89 SS.hold @16:24:27.46 Intake.select :close @16:24:27.61 SSV.dial :exhaust @16:24:28.37 Exhaust.select :open @16:24:28.48 SS.seek 0ml @16:24:35.23 PSV.dial PTV @16:24:36.12 delay 5 seconds @16:24:37.37 Exhaust.select :close @16:24:37.47 SSV.dial CBV @16:24:37.50 CBV.dial SSV @16:24:38.26 Allowing 130s for 25.0ml of remaining 725.0ml @16:24:38.27 Intake.select :open @16:24:40.44 Intake @14.9psia, vacuum from 9.4 to 12.4psi @16:24:40.45 SS.reconfigure :inhaling 0.78ml/s @16:24:40.50 SS.seek 25ml @16:24:41.14 PSV.dial PSR @16:24:41.89 delay 5 seconds @16:24:46.91 PSV.dial PTV @16:24:47.67 delay 5 seconds @16:24:52.69 PSV.dial :air @16:24:53.94 PS.seek 0ml @16:25:15.41 Stroke completed -- waiting for pressure to equalize ... @16:25:15.42 SS.reconfigure :exhale @16:25:15.47 SS.hold @16:25:17.26 washCycleDA #2 of 2 @16:25:17.27 PSR.select :air @16:25:17.29 PSV.dial PSR @16:25:18.66 Pulling 0.5ml of PSR.wash @16:25:18.67 PSR.select :wash @16:25:18.79 PS.reconfigure :default @16:25:18.83 PS.seek 0.5ml @16:25:24.73 delay 5 seconds @16:25:25.24 Intake.select :close @16:25:25.39 SSV.dial :exhaust @16:25:26.15 Exhaust.select :open @16:25:26.27 SS.seek 0ml @16:25:29.75 PSR.select :air @16:25:29.86 PS.seek 2ml @16:25:35.29 Exhaust.select :close @16:25:35.40 SSV.dial CBV @16:25:35.42 CBV.dial SSV @16:25:36.19 Allowing 130s for 25.0ml of remaining 700.0ml @16:25:36.21 Intake.select :open @16:25:38.36 Intake @14.8psia, vacuum from 9.3 to 12.3psi @16:25:38.38 SS.reconfigure :inhaling 0.78ml/s @16:25:38.42 SS.seek 25ml @16:25:46.04 PTV.dial :puck @16:25:46.06 PSV.dial PTV @16:25:46.79 PBV.dial :waste1 @16:25:47.21 PS.seek 1.5ml @16:25:53.14 delay 3 seconds @16:25:56.16 PBV.dial between PSV and waste1 @16:25:56.52 PS.seek 0ml @16:26:12.55 Stroke completed -- waiting for pressure to equalize ... @16:26:12.56 SS.reconfigure :exhale @16:26:12.61 SS.hold @16:26:12.87 delay 2:00 @16:26:23.65 Intake.select :close @16:26:23.79 SSV.dial :exhaust @16:26:24.55 Exhaust.select :open @16:26:24.66 SS.seek 0ml @16:26:33.47 Exhaust.select :close @16:26:33.58 SSV.dial CBV @16:26:33.60 CBV.dial SSV @16:26:34.38 Allowing 130s for 25.0ml of remaining 675.0ml @16:26:34.39 Intake.select :open @16:26:36.55 Intake @14.9psia, vacuum from 9.4 to 12.4psi @16:26:36.57 SS.reconfigure :inhaling 0.78ml/s @16:26:36.61 SS.seek 25ml @16:27:05.81 Vacuum too high -- waiting up to 40 seconds @16:27:05.82 SS.reconfigure :exhale @16:27:05.86 SS.hold @16:27:10.72 Intake @13.5psia, vacuum from 8.0 to 11.0psi @16:27:10.74 SS.reconfigure :inhaling 0.70ml/s @16:27:10.78 SS.seek 25ml @16:27:17.31 Stroke completed -- waiting for pressure to equalize ... @16:27:17.33 SS.reconfigure :exhale @16:27:17.37 SS.hold @16:27:25.13 Intake.select :close @16:27:25.27 SSV.dial :exhaust @16:27:26.02 Exhaust.select :open @16:27:26.13 SS.seek 0ml @16:27:34.97 Exhaust.select :close @16:27:35.08 SSV.dial CBV @16:27:35.10 CBV.dial SSV @16:27:35.86 Allowing 130s for 25.0ml of remaining 650.0ml @16:27:35.88 Intake.select :open @16:27:38.03 Intake @14.9psia, vacuum from 9.4 to 12.4psi @16:27:38.05 SS.reconfigure :inhaling 0.70ml/s @16:27:38.09 SS.seek 25ml @16:28:12.89 recoverPSRreagent08 @16:28:12.90 PTV.dial :puck @16:28:12.92 PBV.dial :waste1 @16:28:13.26 delay 30 seconds @16:28:15.53 Vacuum too high -- waiting up to 40 seconds @16:28:15.54 SS.reconfigure :exhale @16:28:15.58 SS.hold @16:28:20.45 Intake @13.5psia, vacuum from 8.0 to 11.0psi @16:28:20.47 SS.reconfigure :inhaling 0.63ml/s @16:28:20.51 SS.seek 25ml @16:28:21.33 Stroke completed -- waiting for pressure to equalize ... @16:28:21.34 SS.reconfigure :exhale @16:28:21.38 SS.hold @16:28:27.80 Intake.select :close @16:28:27.94 SSV.dial :exhaust @16:28:28.69 Exhaust.select :open @16:28:28.80 SS.seek 0ml @16:28:37.64 Exhaust.select :close @16:28:37.75 SSV.dial CBV @16:28:37.77 CBV.dial SSV @16:28:38.54 Allowing 130s for 25.0ml of remaining 625.0ml @16:28:38.55 Intake.select :open @16:28:40.70 Intake @14.9psia, vacuum from 9.4 to 12.4psi @16:28:40.72 SS.reconfigure :inhaling 0.63ml/s @16:28:40.76 SS.seek 25ml @16:28:43.28 PBV.dial between PSV and waste1 @16:28:43.64 PSR.select :air @16:28:43.65 PSV.dial PSR @16:28:44.44 PS.reconfigure :fast @16:28:44.48 PS.seek 2.5ml @16:29:08.35 PSV.dial PTV @16:29:09.24 delay 5 seconds @16:29:14.26 PSV.dial PSR @16:29:15.01 delay 5 seconds @16:29:20.03 PSV.dial PTV @16:29:20.79 delay 5 seconds @16:29:22.95 Stroke completed -- waiting for pressure to equalize ... @16:29:22.97 SS.reconfigure :exhale @16:29:23.01 SS.hold @16:29:25.85 PSV.dial :air @16:29:26.59 PS.seek 0ml @16:29:34.56 Intake.select :close @16:29:34.70 SSV.dial :exhaust @16:29:35.45 Exhaust.select :open @16:29:35.57 SS.seek 0ml @16:29:44.41 Exhaust.select :close @16:29:44.52 SSV.dial CBV @16:29:44.53 CBV.dial SSV @16:29:45.30 Allowing 130s for 25.0ml of remaining 600.0ml @16:29:45.31 Intake.select :open @16:29:47.47 Intake @14.9psia, vacuum from 9.4 to 12.4psi @16:29:47.49 SS.reconfigure :inhaling 0.63ml/s @16:29:47.53 SS.seek 25ml @16:29:49.98 getPMLReagent08 @16:29:49.99 PRV.dial :air @16:29:50.43 delay 5 seconds @16:29:55.45 PTV.dial :PRVmixing @16:29:56.77 delay 5 seconds @16:30:01.79 PRV.dial :ab2 @16:30:02.26 PTV.dial :PRVmixing @16:30:02.27 PSV.dial :mixloop @16:30:02.73 PS.reconfigure :default @16:30:02.78 PS.seek 0.3ml @16:30:06.69 delay 2 seconds @16:30:08.71 PRV.dial :air @16:30:09.24 PS.seek 0.55ml @16:30:12.54 addPMLReagent08 @16:30:12.55 PSV.dial :air @16:30:13.02 PS.seek 2ml @16:30:28.69 PSV.dial :PML @16:30:29.16 PTV.dial :mixing @16:30:29.72 Stroke completed -- waiting for pressure to equalize ... @16:30:29.73 SS.reconfigure :exhale @16:30:29.77 SS.hold @16:30:29.91 PBV.dial :waste1 @16:30:30.41 PS.seek 1.85ml @16:30:32.72 PBV.dial between PSV and waste1 @16:30:33.09 PS.seek 0ml @16:30:42.41 Intake.select :close @16:30:42.55 SSV.dial :exhaust @16:30:43.30 Exhaust.select :open @16:30:43.41 SS.seek 0ml @16:30:52.48 Exhaust.select :close @16:30:52.59 SSV.dial CBV @16:30:52.61 CBV.dial SSV @16:30:52.85 delay 2 seconds @16:30:53.56 Allowing 130s for 25.0ml of remaining 575.0ml @16:30:53.58 Intake.select :open @16:30:54.87 delay 8:00 @16:30:55.73 Intake @15.0psia, vacuum from 9.5 to 12.5psi @16:30:55.75 SS.reconfigure :inhaling 0.63ml/s @16:30:55.79 SS.seek 25ml @16:31:33.12 Vacuum too high -- waiting up to 40 seconds @16:31:33.13 SS.reconfigure :exhale @16:31:33.17 SS.hold @16:31:39.68 Intake @13.9psia, vacuum from 8.4 to 11.4psi @16:31:39.70 SS.reconfigure :inhaling 0.57ml/s @16:31:39.75 SS.seek 25ml @16:31:44.98 Stroke completed -- waiting for pressure to equalize ... @16:31:44.99 SS.reconfigure :exhale @16:31:45.04 SS.hold @16:31:52.94 Intake.select :close @16:31:53.08 SSV.dial :exhaust @16:31:53.83 Exhaust.select :open @16:31:53.94 SS.seek 0ml @16:32:02.81 Exhaust.select :close @16:32:02.92 SSV.dial CBV @16:32:02.94 CBV.dial SSV @16:32:03.70 Allowing 130s for 25.0ml of remaining 550.0ml @16:32:03.72 Intake.select :open @16:32:05.87 Intake @14.9psia, vacuum from 9.4 to 12.4psi @16:32:05.89 SS.reconfigure :inhaling 0.57ml/s @16:32:05.93 SS.seek 25ml @16:32:52.07 Stroke completed -- waiting for pressure to equalize ... @16:32:52.09 SS.reconfigure :exhale @16:32:52.13 SS.hold @16:33:06.40 Intake.select :close @16:33:06.55 SSV.dial :exhaust @16:33:07.29 Exhaust.select :open @16:33:07.41 SS.seek 0ml @16:33:16.31 Exhaust.select :close @16:33:16.42 SSV.dial CBV @16:33:16.45 CBV.dial SSV @16:33:17.20 Allowing 130s for 25.0ml of remaining 525.0ml @16:33:17.21 Intake.select :open @16:33:19.37 Intake @14.9psia, vacuum from 9.4 to 12.4psi @16:33:19.39 SS.reconfigure :inhaling 0.57ml/s @16:33:19.43 SS.seek 25ml @16:33:56.55 Vacuum too high -- waiting up to 40 seconds @16:33:56.56 SS.reconfigure :exhale @16:33:56.61 SS.hold @16:34:03.15 Intake @13.9psia, vacuum from 8.4 to 11.4psi @16:34:03.17 SS.reconfigure :inhaling 0.51ml/s @16:34:03.21 SS.seek 25ml @16:34:13.34 Stroke completed -- waiting for pressure to equalize ... @16:34:13.35 SS.reconfigure :exhale @16:34:13.39 SS.hold @16:34:23.06 Intake.select :close @16:34:23.20 SSV.dial :exhaust @16:34:23.97 Exhaust.select :open @16:34:24.08 SS.seek 0ml @16:34:32.93 Exhaust.select :close @16:34:33.04 SSV.dial CBV @16:34:33.06 CBV.dial SSV @16:34:33.84 Allowing 130s for 25.0ml of remaining 500.0ml @16:34:33.85 Intake.select :open @16:34:36.01 Intake @14.9psia, vacuum from 9.4 to 12.4psi @16:34:36.03 SS.reconfigure :inhaling 0.51ml/s @16:34:36.07 SS.seek 25ml @16:35:27.52 Stroke completed -- waiting for pressure to equalize ... @16:35:27.53 SS.reconfigure :exhale @16:35:27.58 SS.hold @16:35:44.08 Intake.select :close @16:35:44.23 SSV.dial :exhaust @16:35:44.99 Exhaust.select :open @16:35:45.10 SS.seek 0ml @16:35:53.87 Exhaust.select :close @16:35:53.97 SSV.dial CBV @16:35:53.99 CBV.dial SSV @16:35:54.76 Allowing 130s for 25.0ml of remaining 475.0ml @16:35:54.82 Intake.select :open @16:35:56.98 Intake @14.9psia, vacuum from 9.4 to 12.4psi @16:35:57.00 SS.reconfigure :inhaling 0.51ml/s @16:35:57.05 SS.seek 25ml @16:36:42.30 Vacuum too high -- waiting up to 40 seconds @16:36:42.31 SS.reconfigure :exhale @16:36:42.35 SS.hold @16:36:49.82 Intake @13.6psia, vacuum from 8.1 to 11.1psi @16:36:49.84 SS.reconfigure :inhaling 0.47ml/s @16:36:49.88 SS.seek 25ml @16:36:56.97 Stroke completed -- waiting for pressure to equalize ... @16:36:56.99 SS.reconfigure :exhale @16:36:57.03 SS.hold @16:37:08.08 Intake.select :close @16:37:08.22 SSV.dial :exhaust @16:37:08.98 Exhaust.select :open @16:37:09.09 SS.seek 0ml @16:37:17.96 Exhaust.select :close @16:37:18.07 SSV.dial CBV @16:37:18.10 CBV.dial SSV @16:37:18.85 Allowing 130s for 25.0ml of remaining 450.0ml @16:37:18.87 Intake.select :open @16:37:21.02 Intake @14.9psia, vacuum from 9.4 to 12.4psi @16:37:21.04 SS.reconfigure :inhaling 0.47ml/s @16:37:21.08 SS.seek 25ml @16:38:13.39 Vacuum too high -- waiting up to 40 seconds @16:38:13.40 SS.reconfigure :exhale @16:38:13.44 SS.hold @16:38:22.00 Intake @13.8psia, vacuum from 8.3 to 11.3psi @16:38:22.02 SS.reconfigure :inhaling 0.43ml/s @16:38:22.06 SS.seek 25ml @16:38:26.43 Stroke completed -- waiting for pressure to equalize ... @16:38:26.44 SS.reconfigure :exhale @16:38:26.48 SS.hold @16:38:35.57 Intake.select :close @16:38:35.71 SSV.dial :exhaust @16:38:36.46 Exhaust.select :open @16:38:36.57 SS.seek 0ml @16:38:45.39 Exhaust.select :close @16:38:45.50 SSV.dial CBV @16:38:45.53 CBV.dial SSV @16:38:46.27 Allowing 130s for 25.0ml of remaining 425.0ml @16:38:46.29 Intake.select :open @16:38:48.45 Intake @14.9psia, vacuum from 9.4 to 12.4psi @16:38:48.47 SS.reconfigure :inhaling 0.43ml/s @16:38:48.51 SS.seek 25ml @16:38:54.89 recoverPRVreagent08 @16:38:54.90 PTV.dial :mixing @16:38:54.91 PBV.dial :waste1 @16:38:55.28 delay 30 seconds @16:39:25.31 PBV.dial between PSV and waste1 @16:39:25.67 PSV.dial :air @16:39:26.14 PS.reconfigure :fast @16:39:26.19 PS.seek 2.5ml @16:39:50.12 PSV.dial :mixloop @16:39:50.60 delay 2 seconds @16:39:50.62 Stroke completed -- waiting for pressure to equalize ... @16:39:50.63 SS.reconfigure :exhale @16:39:50.68 SS.hold @16:39:52.68 PSV.dial :air @16:39:53.15 delay 2 seconds @16:39:55.17 PSV.dial :mixloop @16:39:55.64 delay 2 seconds @16:39:57.65 PSV.dial :air @16:39:58.13 PS.seek 0ml @16:40:09.00 Intake.select :close @16:40:09.14 SSV.dial :exhaust @16:40:09.88 Exhaust.select :open @16:40:09.99 SS.seek 0ml @16:40:18.88 Exhaust.select :close @16:40:18.98 SSV.dial CBV @16:40:19.01 CBV.dial SSV @16:40:19.77 Allowing 130s for 25.0ml of remaining 400.0ml @16:40:19.78 Intake.select :open @16:40:21.50 washCycleDA #1 of 3 @16:40:21.51 PSR.select :air @16:40:21.52 PSV.dial PSR @16:40:21.94 Intake @15.0psia, vacuum from 9.5 to 12.5psi @16:40:21.95 SS.reconfigure :inhaling 0.43ml/s @16:40:22.00 SS.seek 25ml @16:40:22.89 Pulling 0.5ml of PSR.wash @16:40:22.90 PSR.select :wash @16:40:23.02 PS.reconfigure :default @16:40:23.06 PS.seek 0.5ml @16:40:28.94 delay 5 seconds @16:40:33.95 PSR.select :air @16:40:34.07 PS.seek 2ml @16:40:50.27 PTV.dial :puck @16:40:51.03 PSV.dial PTV @16:40:51.78 PBV.dial :waste1 @16:40:52.21 PS.seek 1.5ml @16:40:58.12 delay 3 seconds @16:41:01.14 PBV.dial between PSV and waste1 @16:41:01.52 PS.seek 0ml @16:41:14.20 Vacuum too high -- waiting up to 40 seconds @16:41:14.21 SS.reconfigure :exhale @16:41:14.25 SS.hold @16:41:17.72 delay 2:00 @16:41:23.31 Intake @14.1psia, vacuum from 8.6 to 11.6psi @16:41:23.33 SS.reconfigure :inhaling 0.38ml/s @16:41:23.38 SS.seek 25ml @16:41:35.09 Stroke completed -- waiting for pressure to equalize ... @16:41:35.11 SS.reconfigure :exhale @16:41:35.15 SS.hold @16:41:47.72 Intake.select :close @16:41:47.86 SSV.dial :exhaust @16:41:48.63 Exhaust.select :open @16:41:48.74 SS.seek 0ml @16:41:57.66 Exhaust.select :close @16:41:57.76 SSV.dial CBV @16:41:57.79 CBV.dial SSV @16:41:58.55 Allowing 130s for 25.0ml of remaining 375.0ml @16:41:58.56 Intake.select :open @16:42:00.72 Intake @15.0psia, vacuum from 9.5 to 12.5psi @16:42:00.74 SS.reconfigure :inhaling 0.38ml/s @16:42:00.78 SS.seek 25ml @16:43:10.29 Vacuum too high -- waiting up to 40 seconds @16:43:10.30 SS.reconfigure :exhale @16:43:10.34 SS.hold @16:43:17.76 recoverPSRreagent08 @16:43:17.78 PTV.dial :puck @16:43:17.79 PBV.dial :waste1 @16:43:18.15 delay 30 seconds @16:43:21.47 Intake @14.4psia, vacuum from 8.9 to 11.9psi @16:43:21.48 SS.reconfigure :inhaling 0.34ml/s @16:43:21.53 SS.seek 25ml @16:43:21.97 Stroke completed -- waiting for pressure to equalize ... @16:43:21.98 SS.reconfigure :exhale @16:43:22.02 SS.hold @16:43:31.69 Intake.select :close @16:43:31.85 SSV.dial :exhaust @16:43:32.61 Exhaust.select :open @16:43:32.72 SS.seek 0ml @16:43:41.59 Exhaust.select :close @16:43:41.70 SSV.dial CBV @16:43:41.72 CBV.dial SSV @16:43:42.50 Allowing 130s for 25.0ml of remaining 350.0ml @16:43:42.51 Intake.select :open @16:43:44.67 Intake @15.1psia, vacuum from 9.6 to 12.6psi @16:43:44.69 SS.reconfigure :inhaling 0.34ml/s @16:43:44.73 SS.seek 25ml @16:43:48.17 PBV.dial between PSV and waste1 @16:43:48.54 PSR.select :air @16:43:48.56 PSV.dial PSR @16:43:49.32 PS.reconfigure :fast @16:43:49.91 PS.seek 2.5ml @16:44:13.62 PSV.dial PTV @16:44:14.38 delay 5 seconds @16:44:19.40 PSV.dial PSR @16:44:20.16 delay 5 seconds @16:44:25.18 PSV.dial PTV @16:44:25.94 delay 5 seconds @16:44:30.96 PSV.dial :air @16:44:31.72 PS.seek 0ml @16:44:55.21 washCycleDA #2 of 3 @16:44:55.22 PSR.select :air @16:44:55.24 PSV.dial PSR @16:44:56.61 Pulling 0.5ml of PSR.wash @16:44:56.62 PSR.select :wash @16:44:56.73 PS.reconfigure :default @16:44:56.77 PS.seek 0.5ml @16:45:02.77 delay 5 seconds @16:45:05.62 Stroke completed -- waiting for pressure to equalize ... @16:45:05.63 SS.reconfigure :exhale @16:45:05.68 SS.hold @16:45:07.79 PSR.select :air @16:45:07.91 PS.seek 2ml @16:45:24.11 PTV.dial :puck @16:45:24.12 PSV.dial PTV @16:45:24.86 PBV.dial :waste1 @16:45:25.30 PS.seek 1.5ml @16:45:26.02 Intake.select :close @16:45:26.16 SSV.dial :exhaust @16:45:26.92 Exhaust.select :open @16:45:27.03 SS.seek 0ml @16:45:31.30 delay 3 seconds @16:45:34.32 PBV.dial between PSV and waste1 @16:45:34.70 PS.seek 0ml @16:45:36.12 Exhaust.select :close @16:45:36.23 SSV.dial CBV @16:45:36.25 CBV.dial SSV @16:45:37.03 Allowing 130s for 25.0ml of remaining 325.0ml @16:45:37.04 Intake.select :open @16:45:39.20 Intake @15.1psia, vacuum from 9.6 to 12.6psi @16:45:39.22 SS.reconfigure :inhaling 0.34ml/s @16:45:39.26 SS.seek 25ml @16:45:50.83 delay 2:00 @16:47:00.20 Stroke completed -- waiting for pressure to equalize ... @16:47:00.21 SS.reconfigure :exhale @16:47:00.25 SS.hold @16:47:26.67 Intake.select :close @16:47:26.81 SSV.dial :exhaust @16:47:27.58 Exhaust.select :open @16:47:27.69 SS.seek 0ml @16:47:36.54 Exhaust.select :close @16:47:36.65 SSV.dial CBV @16:47:36.70 CBV.dial SSV @16:47:37.42 Allowing 130s for 25.0ml of remaining 300.0ml @16:47:37.43 Intake.select :open @16:47:39.58 Intake @15.1psia, vacuum from 9.6 to 12.6psi @16:47:39.60 SS.reconfigure :inhaling 0.34ml/s @16:47:39.64 SS.seek 25ml @16:47:50.86 recoverPSRreagent08 @16:47:50.87 PTV.dial :puck @16:47:51.31 PBV.dial :waste1 @16:47:51.75 delay 30 seconds @16:48:21.78 PBV.dial between PSV and waste1 @16:48:22.14 PSR.select :air @16:48:22.16 PSV.dial PSR @16:48:22.93 PS.reconfigure :fast @16:48:22.97 PS.seek 2.5ml @16:48:32.16 Vacuum too high -- waiting up to 40 seconds @16:48:32.17 SS.reconfigure :exhale @16:48:32.22 SS.hold @16:48:44.76 Intake @14.7psia, vacuum from 9.2 to 12.2psi @16:48:44.78 SS.reconfigure :inhaling 0.31ml/s @16:48:44.82 SS.seek 25ml @16:48:46.83 PSV.dial PTV @16:48:47.60 delay 5 seconds @16:48:52.61 PSV.dial PSR @16:48:53.38 delay 5 seconds @16:48:58.40 PSV.dial PTV @16:48:59.16 delay 5 seconds @16:49:04.18 PSV.dial :air @16:49:04.94 PS.seek 0ml @16:49:15.24 Stroke completed -- waiting for pressure to equalize ... @16:49:15.25 SS.reconfigure :exhale @16:49:15.30 SS.hold @16:49:28.20 washCycleDA #3 of 3 @16:49:28.21 PSR.select :air @16:49:28.23 PSV.dial PSR @16:49:29.60 Pulling 0.5ml of PSR.wash @16:49:29.61 PSR.select :wash @16:49:29.72 PS.reconfigure :default @16:49:29.76 PS.seek 0.5ml @16:49:35.67 delay 5 seconds @16:49:37.61 Intake.select :close @16:49:37.76 SSV.dial :exhaust @16:49:38.52 Exhaust.select :open @16:49:38.64 SS.seek 0ml @16:49:40.69 PSR.select :air @16:49:40.80 PS.seek 2ml @16:49:47.76 Exhaust.select :close @16:49:47.87 SSV.dial CBV @16:49:47.88 CBV.dial SSV @16:49:48.66 Allowing 130s for 25.0ml of remaining 275.0ml @16:49:48.68 Intake.select :open @16:49:50.83 Intake @15.2psia, vacuum from 9.7 to 12.7psi @16:49:50.85 SS.reconfigure :inhaling 0.31ml/s @16:49:50.89 SS.seek 25ml @16:49:56.86 PTV.dial :puck @16:49:56.87 PSV.dial PTV @16:49:57.61 PBV.dial :waste1 @16:49:58.04 PS.seek 1.5ml @16:50:03.98 delay 3 seconds @16:50:07.00 PBV.dial between PSV and waste1 @16:50:07.38 PS.seek 0ml @16:50:23.55 delay 2:00 @16:51:00.37 Vacuum too high -- waiting up to 40 seconds @16:51:00.39 SS.reconfigure :exhale @16:51:00.43 SS.hold @16:51:13.61 Intake @14.8psia, vacuum from 9.3 to 12.3psi @16:51:13.63 SS.reconfigure :inhaling 0.28ml/s @16:51:13.67 SS.seek 25ml @16:51:34.35 Stroke completed -- waiting for pressure to equalize ... @16:51:34.36 SS.reconfigure :exhale @16:51:34.40 SS.hold @16:51:56.73 Intake.select :close @16:51:56.87 SSV.dial :exhaust @16:51:57.61 Exhaust.select :open @16:51:57.72 SS.seek 0ml @16:52:06.53 Exhaust.select :close @16:52:06.64 SSV.dial CBV @16:52:06.66 CBV.dial SSV @16:52:07.43 Allowing 130s for 25.0ml of remaining 250.0ml @16:52:07.44 Intake.select :open @16:52:09.59 Intake @15.2psia, vacuum from 9.7 to 12.7psi @16:52:09.61 SS.reconfigure :inhaling 0.28ml/s @16:52:09.65 SS.seek 25ml @16:52:23.56 recoverPSRreagent08 @16:52:23.57 PTV.dial :puck @16:52:23.59 PBV.dial :waste1 @16:52:23.95 delay 30 seconds @16:52:53.98 PBV.dial between PSV and waste1 @16:52:54.34 PSR.select :air @16:52:54.36 PSV.dial PSR @16:52:55.13 PS.reconfigure :fast @16:52:55.17 PS.seek 2.5ml @16:53:19.07 PSV.dial PTV @16:53:19.84 delay 5 seconds @16:53:24.86 PSV.dial PSR @16:53:25.60 delay 5 seconds @16:53:30.62 PSV.dial PTV @16:53:31.39 delay 5 seconds @16:53:36.41 PSV.dial :air @16:53:37.28 PS.seek 0ml @16:53:39.00 Vacuum too high -- waiting up to 40 seconds @16:53:39.01 SS.reconfigure :exhale @16:53:39.05 SS.hold @16:53:51.73 Intake @14.5psia, vacuum from 9.0 to 12.0psi @16:53:51.75 SS.reconfigure :inhaling 0.25ml/s @16:53:51.80 SS.seek 25ml @16:54:00.45 Gate.power :camera,:ON @16:54:00.46 Mixing DA Substrates @16:54:00.47 PSR.select :air @16:54:00.49 PSV.dial PSR @16:54:02.01 Pulling 0.25ml of PSR.sub1 @16:54:02.04 PSR.select :sub1 @16:54:02.23 PS.reconfigure :default @16:54:02.33 PS.seek 0.25ml @16:54:04.83 Stroke completed -- waiting for pressure to equalize ... @16:54:04.84 SS.reconfigure :exhale @16:54:04.88 SS.hold @16:54:05.76 delay 5 seconds @16:54:10.86 Pulling 0.25ml of PSR.sub2 @16:54:10.87 PSR.select :sub2 @16:54:10.99 PS.seek 0.5ml @16:54:14.35 delay 5 seconds @16:54:17.53 SS.reconfigure :default @16:54:17.59 SS.hold @16:54:17.70 Intake.select :close @16:54:17.84 ShallowSampler::Clogged in WCR -- Filtering too Slowly after 775.0ml @16:54:17.86 SSV.dial :exhaust @16:54:18.61 Exhaust.select :open @16:54:18.72 SS.reconfigure :exhale @16:54:18.77 SS.seek 0ml @16:54:19.37 PSR.select :air @16:54:19.48 PS.seek 2ml @16:54:27.72 Exhaust.select :close @16:54:27.83 Sampled 775.0ml @16:54:27.85 Drying puck before fixing for 20:00 @16:54:27.86 CBV.dial :waste2b @16:54:28.61 delay 1:00 @16:54:35.66 PTV.dial :puck @16:54:35.68 PSV.dial PTV @16:54:36.55 PBV.dial :waste1 @16:54:36.99 PS.seek 1.5ml @16:54:42.98 delay 3 seconds @16:54:45.99 PBV.dial between PSV and waste1 @16:54:46.37 PS.seek 0ml @16:55:02.60 delay 10 seconds @16:55:12.63 recoverPSRreagent08 @16:55:12.64 PTV.dial :puck @16:55:12.65 PBV.dial :waste1 @16:55:13.01 delay 30 seconds @16:55:28.63 CSV.dial :air @16:55:29.08 CS.reconfigure :fast @16:55:29.13 CS.seek 10ml @16:55:43.02 PBV.dial between PSV and waste1 @16:55:43.40 PSR.select :air @16:55:43.42 PSV.dial PSR @16:55:44.17 PS.reconfigure :fast @16:55:44.21 PS.seek 2.5ml @16:55:53.77 Pumping wcr puck from tube 4 up to 35psi... @16:55:53.78 CBV.dial SSV @16:55:55.11 CBV.dial between CSV and waste2b @16:55:56.09 CSV.dial between air and CTV @16:55:56.41 CS.seek 7.389ml @16:56:03.30 CTV.dial CSV,avoiding: :air @16:56:03.32 CSV.dial CTV @16:56:04.16 CS.reconfigure :pressurize @16:56:04.21 CS.seek 0ml @16:56:08.26 PSV.dial PTV @16:56:09.02 delay 5 seconds @16:56:14.04 PSV.dial PSR @16:56:14.80 delay 5 seconds @16:56:18.16 CBV.dial :waste2b @16:56:18.50 CS.reconfigure :default @16:56:18.55 CS.seek 0ml @16:56:19.82 PSV.dial PTV @16:56:20.59 delay 5 seconds @16:56:24.82 delay 1:00 @16:56:25.60 PSV.dial :air @16:56:26.37 PS.seek 0ml @16:56:49.85 Additional air clear @16:56:49.86 PSR.select :air @16:56:49.88 PSV.dial PSR @16:56:51.25 Pulling 2ml of PSR.air @16:56:51.26 PSR.select :air @16:56:51.28 PS.reconfigure :default @16:56:51.32 PS.seek 2ml @16:57:12.50 delay 5 seconds @16:57:17.52 PTV.dial :puck @16:57:17.54 PSV.dial PTV @16:57:18.29 PBV.dial :waste1 @16:57:18.72 PS.seek 1.5ml @16:57:24.63 delay 3 seconds @16:57:24.85 CTV.dial :air,avoiding: :intake @16:57:26.19 Fix #1 of 2 (with RNAlater) for 10:00 @16:57:26.20 CSV.dial CSR @16:57:26.69 Pulling 2ml of CSR.RNAlater @16:57:26.70 CSR.select :RNAlater @16:57:26.81 CS.seek 2ml @16:57:27.65 PBV.dial between PSV and waste1 @16:57:28.02 delay 1 second @16:57:29.04 PSV.dial :waste1 @16:57:30.10 PS.seek 0ml @16:57:32.73 delay 5 seconds @16:57:37.75 CSR.select :air @16:57:38.35 CS.reconfigure :fast @16:57:38.40 CS.seek 10ml @16:57:46.30 Processing Puck Heater at 28.1C before imaging @16:57:46.31 PH.coast @16:57:46.44 Imaging da puck in Processing Clamp while storing it to tube 3 @16:57:46.60 SC.reconfigure :tight @16:57:46.64 SC.seek 3 @16:57:50.26 SC.configure :limp @16:57:50.38 SE.reconfigure :place @16:57:50.51 SE.seek :beamBreak @16:57:50.57 Elbow.reconfigure :tight @16:57:50.62 Elbow.seek PC @16:57:53.35 SE.configure :default @16:57:53.40 SE.seek :place @16:57:54.39 Elbow.reconfigure :loose @16:57:54.48 Forearm.seek PC @16:57:55.41 Hand.close @16:57:55.96 PC.seek :open @16:57:58.07 Pumping wcr puck from tube 4 up to 35psi... @16:57:58.09 CBV.dial SSV @16:57:59.41 CBV.dial between CSV and waste2b @16:58:00.38 CSV.dial between air and CTV @16:58:00.76 Forearm.seek :retract @16:58:00.99 CS.seek 7.234ml @16:58:01.70 Elbow.reconfigure :tight @16:58:01.74 Elbow.seek 3 @16:58:04.30 Forearm.reconfigure :tight @16:58:04.34 Forearm.seek 3 + 450 counts @16:58:05.30 Hand.open @16:58:06.02 SE.seek -0.26 Pucks below Top Plate @16:58:06.30 SE.reconfigure :seal @16:58:06.35 SE.seek -1.22 Pucks below Top Plate @16:58:06.89 Lights, camera, ACTION! @16:58:06.93 PuckCamera: snap -bin=2,2 -auto /var/log/esp/da23feb1416h1000ml.tif @16:58:08.09 CTV.dial CSV,avoiding: :air @16:58:08.11 CSV.dial CTV @16:58:09.57 CS.reconfigure :pressurize @16:58:09.65 CS.seek 0ml @16:58:23.25 delay 10:00 @16:58:26.39 Waiting >20 seconds for da to relinquish FlushPuck @16:58:29.23 SX HX9/16 (USB:17): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 19.004 seconds /var/log/esp/da23feb1416h1000ml.tif: TIFF Upload Complete @16:58:29.25 PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da23feb1416h1000ml15s.tif @16:58:44.86 SX HX9/16 (USB:17): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15 seconds /var/log/esp/midres/da23feb1416h1000ml15s.tif: TIFF Upload Complete @16:58:44.89 PuckCamera: snap -bin=2,2 20.0 /var/log/esp/midres/da23feb1416h1000ml20s.tif @16:59:05.54 SX HX9/16 (USB:17): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 20 seconds /var/log/esp/midres/da23feb1416h1000ml20s.tif: TIFF Upload Complete @16:59:05.56 Gate.power :camera,:OFF @16:59:05.58 DAfans.email "19.004 sec auto-exposure",Subject: "da23feb1416h1000ml images!" @16:59:05.83 Elbow.seek 3 @16:59:06.01 Forearm.seek 3 + 450 counts @16:59:06.11 SE.reconfigure :gently @16:59:06.21 SE.seek -0.51 Pucks below Top Plate @16:59:06.99 Elbow.reconfigure :limp @16:59:07.04 Forearm.reconfigure :loose @16:59:07.08 Forearm.seek 3 @16:59:07.34 Elbow.hold @16:59:07.42 Elbow.reconfigure :tight @16:59:07.47 SE.reconfigure :default @16:59:07.52 SE.seek -0.32 Pucks below Top Plate @16:59:07.87 Forearm.force -20 @16:59:07.93 Forearm.seek 3 + 318 counts @16:59:08.24 Forearm.reconfigure :tight @16:59:08.29 Forearm.seek 3 + 450 counts @16:59:09.06 SE.seek :down @16:59:10.19 Forearm.reconfigure :loose @16:59:10.26 Forearm.seek :retract @16:59:11.85 SE.stop @16:59:12.07 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @16:59:12.44 Elbow.seek FlushPuck::Garage @16:59:14.43 Elbow.reconfigure :loose @16:59:14.63 Forearm.seek :garage @16:59:15.98 Hand.close @16:59:16.85 Forearm.seek :retract @16:59:17.91 Elbow.reconfigure :tight @16:59:18.01 Elbow.seek PC @16:59:19.39 Elbow.reconfigure :loose @16:59:19.59 Forearm.reconfigure :tight @16:59:19.68 Forearm.seek PC @16:59:21.22 PC.seek :closed @16:59:25.99 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.61 @16:59:26.23 Hand.open @16:59:26.98 Forearm.reconfigure :loose @16:59:27.06 Forearm.seek :retract @16:59:28.10 PSR.select :air @16:59:28.14 PSV.dial PSR @16:59:28.76 Pulling 0.5ml of PSR.flush @16:59:28.78 PSR.select :flush @16:59:28.96 PS.seek 0.5ml @16:59:35.00 delay 5 seconds @16:59:40.05 PSR.select :air @16:59:40.23 PS.seek 2.5ml @16:59:41.64 upsync @17:00:01.56 delay 1 second @17:00:02.58 PRV.dial :waste2,avoiding: :ab2 @17:00:04.44 PTV.dial :PRVmixing @17:00:05.75 PSV.dial :mixloop @17:00:06.79 PS.seek :empty @17:00:33.12 PSR.select :air @17:00:33.14 PSV.dial PSR @17:00:34.24 Pulling 0.5ml of PSR.flush @17:00:34.25 PSR.select :flush @17:00:34.37 PS.seek 0.5ml @17:00:40.28 delay 5 seconds @17:00:45.30 PSR.select :air @17:00:45.42 PS.seek 2.5ml @17:01:06.72 delay 1 second @17:01:07.74 PBV.dial :waste1 @17:01:08.11 PTV.dial :mixing @17:01:08.86 PSV.dial :mixloop @17:01:09.91 PS.seek :empty @17:01:36.25 PSV.dial PSR @17:01:37.30 PSR.select :flush @17:01:37.41 PS.seek 1ml @17:01:48.47 PSR.select :air @17:01:48.58 PS.seek 2.5ml @17:02:04.68 PSV.dial PTV @17:02:05.45 PTV.dial :puck @17:02:06.19 PBV.dial :waste1 @17:02:06.21 PS.seek 0ml @17:02:32.62 PSV.dial PSR @17:02:33.39 PSR.select :flush @17:02:33.50 PS.seek 0.5ml @17:02:39.37 PSR.select :air @17:02:39.49 PS.seek 2.5ml @17:03:00.75 PSV.dial PTV @17:03:01.51 PBV.dial PSV @17:03:02.08 PS.seek 0ml @17:03:28.62 PSV.dial PBV @17:03:29.10 PS.seek 2.5ml @17:03:55.46 PSV.dial :waste1 @17:03:56.21 PS.seek 0ml @17:04:22.59 PSV.dial PSR @17:04:23.06 PSR.select :air @17:04:23.08 PS.seek 2.5ml @17:04:49.46 PSV.dial PTV @17:04:50.70 PBV.dial PSV @17:04:50.72 PS.seek 0ml @17:05:17.24 PSV.dial PBV @17:05:17.72 PS.seek 2.5ml @17:05:44.06 PSV.dial :waste1 @17:05:44.80 PS.seek 0ml @17:06:11.15 PSV.dial PSR @17:06:11.62 PSR.select :flush @17:06:11.74 PS.seek 1ml @17:06:22.80 PSR.select :air @17:06:22.92 PS.seek 2.5ml @17:06:39.09 PSV.dial PTV @17:06:39.82 PTV.dial :puck @17:06:39.84 PBV.dial :waste1 @17:06:40.39 PS.seek 0ml @17:07:06.74 PSV.dial PSR @17:07:07.49 PSR.select :flush @17:07:07.61 PS.seek 0.5ml @17:07:13.49 PSR.select :air @17:07:13.61 PS.seek 2.5ml @17:07:34.80 PSV.dial PTV @17:07:35.55 PBV.dial PSV @17:07:36.12 PS.seek 0ml @17:08:02.46 PSV.dial PBV @17:08:02.94 PS.seek 2.5ml @17:08:23.28 CBV.dial :waste2b @17:08:23.61 CS.reconfigure :default @17:08:23.66 CS.seek 0ml @17:08:29.24 PSV.dial :waste1 @17:08:29.98 delay 1:00 @17:08:30.02 PS.seek 0ml @17:08:56.36 PSV.dial PSR @17:08:56.83 PSR.select :air @17:08:56.86 PS.seek 2.5ml @17:09:23.27 PSV.dial PTV @17:09:24.01 PBV.dial PSV @17:09:24.03 PS.seek 0ml @17:09:30.00 CTV.dial :air,avoiding: :intake @17:09:31.32 Fix #2 of 2 (with RNAlater) for 10:00 @17:09:31.33 CSV.dial CSR @17:09:31.93 Pulling 1.999ml of CSR.RNAlater @17:09:31.94 CSR.select :RNAlater @17:09:32.05 CS.seek 2ml @17:09:38.06 delay 5 seconds @17:09:43.08 CSR.select :air @17:09:43.19 CS.reconfigure :fast @17:09:43.24 CS.seek 10ml @17:09:50.58 PSV.dial PBV @17:09:51.06 PS.seek 2.5ml @17:10:02.84 Pumping wcr puck from tube 4 up to 35psi... @17:10:02.86 CBV.dial SSV @17:10:04.16 CBV.dial between CSV and waste2b @17:10:05.10 CSV.dial between air and CTV @17:10:05.69 CS.seek 7.205ml @17:10:12.88 CTV.dial CSV,avoiding: :air @17:10:12.90 CSV.dial CTV @17:10:14.30 CS.reconfigure :pressurize @17:10:14.35 CS.seek 0ml @17:10:17.46 PSV.dial :waste1 @17:10:18.20 PS.seek 0ml @17:10:33.25 CSV.dial :air @17:10:33.70 CS.reconfigure :fast @17:10:33.75 CS.seek 10ml @17:10:44.62 Garaging FlushPuck in Processing Clamp @17:10:44.69 Elbow.reconfigure :tight @17:10:44.73 Elbow.seek PC @17:10:45.06 Elbow.reconfigure :loose @17:10:45.15 Forearm.seek PC @17:10:46.15 Hand.close @17:10:46.70 PC.seek :open @17:10:51.41 Forearm.seek :retract @17:10:52.37 Elbow.reconfigure :tight @17:10:52.42 Elbow.seek :garage @17:10:53.72 Elbow.reconfigure :loose @17:10:54.27 Forearm.reconfigure :tight @17:10:54.32 Forearm.seek :garage @17:10:55.34 Hand.open @17:10:56.01 Forearm.reconfigure :loose @17:10:56.05 Forearm.seek :retract @17:10:58.12 CSV.dial between air and CTV @17:10:58.44 CS.seek 4.083ml @17:11:12.63 CTV.dial CSV,avoiding: :air @17:11:12.65 CSV.dial CTV @17:11:12.96 CS.reconfigure :pressurize @17:11:13.00 CS.seek 0ml @17:11:23.93 CSV.dial :air @17:11:24.39 CS.reconfigure :fast @17:11:24.44 CS.seek 10ml @17:11:48.58 CSV.dial between air and CTV @17:11:48.91 CS.seek 4.453ml @17:12:02.16 CTV.dial CSV,avoiding: :air @17:12:02.18 CSV.dial CTV @17:12:02.49 CS.reconfigure :pressurize @17:12:02.53 CS.seek 0ml @17:12:14.40 CSV.dial :air @17:12:14.86 CS.reconfigure :fast @17:12:14.91 CS.seek 10ml @17:12:38.96 CSV.dial between air and CTV @17:12:39.29 CS.seek 4.618ml @17:12:52.16 CTV.dial CSV,avoiding: :air @17:12:52.18 CSV.dial CTV @17:12:52.48 CS.reconfigure :pressurize @17:12:52.53 CS.seek 0ml @17:13:04.84 CSV.dial :air @17:13:05.29 CS.reconfigure :default @17:13:05.34 CS.hold @17:13:05.40 ShallowSampler::Error in MAIN -- Puck.from 4, :wcr did not reach 35psi after pumping in 40ml of gas @17:13:05.41 Waiting to Resume from Checkpoint "Pumping wcr puck from tube 4 up to 35psi..." @17:13:05.43 BadNews.email "Puck.from 4, :wcr did not reach 35psi after pumping in 40ml of gas",Subject: "ShallowSampler::Error in MAIN" @17:13:10.49 upsync @17:29:06.75 Session Begun @17:29:10.38 -> trouble @17:29:34.74 -> MainThread.resume @17:29:34.76
Resuming Thread (suspended since 17:10:02) @17:29:34.78 Pumping wcr puck from tube 4 up to 35psi... @17:29:34.89 CBV.dial SSV @17:29:36.38 CBV.dial between CSV and waste2b @17:29:37.35 CSV.dial between air and CTV @17:29:37.69 CS.reconfigure :fast @17:29:37.78 CS.seek 5.069ml @17:29:50.92 CTV.dial CSV,avoiding: :air @17:29:50.94
CSV.dial CTV @17:29:51.28 CS.reconfigure :pressurize @17:29:51.35 CS.seek 0ml @17:30:04.91 CSV.dial :air @17:30:05.39 CS.reconfigure :fast @17:30:05.46 CS.seek 10ml @17:30:29.81 CSV.dial between air and CTV @17:30:30.74 CS.seek 5.111ml @17:30:42.61 CTV.dial CSV,avoiding: :air @17:30:42.63
CSV.dial CTV @17:30:42.97 CS.reconfigure :pressurize @17:30:43.03 CS.seek 0ml @17:30:56.63 CSV.dial :air @17:30:57.11 CS.reconfigure :fast @17:30:57.17 CS.seek 10ml @17:31:21.41 CSV.dial between air and CTV @17:31:21.75 CS.seek 4.708ml @17:31:34.48 CTV.dial CSV,avoiding: :air @17:31:34.50
CSV.dial CTV @17:31:34.84 CS.reconfigure :pressurize @17:31:34.91 CS.seek 0ml @17:31:47.44 CSV.dial :air @17:31:47.92 CS.reconfigure :fast @17:31:47.99 CS.seek 10ml @17:32:12.22 CSV.dial between air and CTV @17:32:12.55 CS.seek 4.917ml @17:32:24.82 CTV.dial CSV,avoiding: :air @17:32:24.85
CSV.dial CTV @17:32:25.17 CS.reconfigure :pressurize @17:32:25.23 CS.seek 0ml @17:32:38.32 CSV.dial :air @17:32:38.77 CS.reconfigure :default @17:32:38.83 CS.hold @17:32:38.91 ShallowSampler::Error in MAIN -- Puck.from 4, :wcr did not reach 35psi after pumping in 40ml of gas @17:32:38.93 Waiting to Resume from Checkpoint "Pumping wcr puck from tube 4 up to 35psi..." @17:32:38.95 BadNews.email "Puck.from 4, :wcr did not reach 35psi after pumping in 40ml of gas",Subject: "ShallowSampler::Error in MAIN" @17:32:44.11 upsync @17:33:02.68 -> MainThread.resume @17:33:02.70
Resuming Thread (suspended since 17:10:02) @17:33:02.73 Pumping wcr puck from tube 4 up to 35psi... @17:33:02.83 CBV.dial SSV @17:33:04.29 CBV.dial between CSV and waste2b @17:33:05.27 CSV.dial between air and CTV @17:33:05.61 CS.reconfigure :fast @17:33:05.68 CS.seek 5.462ml @17:33:19.70 CTV.dial CSV,avoiding: :air @17:33:19.73
CSV.dial CTV @17:33:20.06 CS.reconfigure :pressurize @17:33:20.13 CS.seek 0ml @17:33:34.69 CSV.dial :air @17:33:35.17 CS.reconfigure :fast @17:33:35.23 CS.seek 10ml @17:33:59.34 CSV.dial between air and CTV @17:33:59.68 CS.seek 5.59ml @17:34:10.45 CTV.dial CSV,avoiding: :air @17:34:10.47
CSV.dial CTV @17:34:10.81 CS.reconfigure :pressurize @17:34:10.88 CS.seek 0ml @17:34:23.87 delay 10:00 @17:44:23.89 CBV.dial :waste2b @17:44:24.23 CS.reconfigure :default @17:44:24.29 CS.seek 0ml @17:44:27.34 delay 1:00 @17:45:27.36 CTV.dial :air,avoiding: :intake @17:45:28.75 Storing wcr puck in Collection Clamp to tube 3 @17:45:29.15 Elbow.reconfigure :tight @17:45:29.21 Elbow.seek 3 @17:45:31.08 Forearm.reconfigure :tight @17:45:31.14 Forearm.seek 3 + 450 counts @17:45:32.20 SE.reconfigure :pick @17:45:32.27 SE.seek :beamBreak @17:45:35.32 SE.configure :default @17:45:35.49 Elbow.reconfigure :limp @17:45:35.55 Forearm.reconfigure :loose @17:45:35.61 Forearm.seek 3 @17:45:35.84 Elbow.hold @17:45:35.92 Elbow.reconfigure :tight @17:45:36.03 SE.seek 0.17 Pucks below Top Plate @17:45:36.46 Forearm.force -20 @17:45:36.52 Forearm.seek 3 + 308 counts @17:45:36.90 Forearm.reconfigure :tight @17:45:36.96 Forearm.seek 3 + 450 counts @17:45:37.17 SE.seek 1.17 Pucks below Top Plate @17:45:37.70 Forearm.reconfigure :loose @17:45:37.77 Forearm.seek :retract @17:45:38.70 Elbow.seek CC @17:45:40.34 Elbow.reconfigure :loose @17:45:40.45 Forearm.seek CC @17:45:41.43 Hand.close @17:45:42.04 CC.seek :open @17:45:46.77 Forearm.seek :retract @17:45:47.76 Elbow.reconfigure :tight @17:45:47.83 Elbow.seek 3 @17:45:49.62 Elbow.reconfigure :limp @17:45:49.69 Forearm.seek 3 @17:45:50.70 Elbow.hold @17:45:50.78 Elbow.reconfigure :tight @17:45:50.85 Hand.open @17:45:51.53 Forearm.force -20 @17:45:51.59 Forearm.seek 3 + 318 counts @17:45:51.88 Forearm.reconfigure :tight @17:45:51.94 Forearm.seek 3 + 450 counts @17:45:52.22 SE.seek :down @17:45:53.34
Forearm.reconfigure :loose @17:45:53.41 Forearm.seek :retract @17:45:54.84 SE.stop @17:45:54.90
Starting finalPurge @17:45:54.92 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @17:45:55.13 Elbow.seek FlushPuck::Garage @17:45:57.01 Elbow.reconfigure :loose @17:45:57.11 Forearm.seek :garage @17:45:58.10 Hand.close @17:45:58.78 Forearm.seek :retract @17:45:59.78 Elbow.reconfigure :tight @17:45:59.84 Elbow.seek CC @17:46:02.81 Elbow.reconfigure :loose @17:46:02.92 Forearm.reconfigure :tight @17:46:02.98 Forearm.seek CC @17:46:04.16 CC.seek :closed @17:46:08.72 Collection Clamp onSpring/offSpring for 14/27 samples, on/off torque 2.20 @17:46:08.82 Hand.open @17:46:09.48 Forearm.reconfigure :loose @17:46:09.54 Forearm.seek :retract @17:46:10.48 Flushing the Collection Valve::Manifold @17:46:10.62 CSV.dial CSR @17:46:11.12 Pulling 5ml of CSR.flush @17:46:11.13 CSR.select :flush @17:46:11.28 CS.seek 5ml @17:46:25.09 delay 5 seconds @17:46:30.10 CSR.select :air @17:46:30.27 CS.reconfigure :fast @17:46:30.33 CS.seek 7.501ml @17:46:37.08 CSV.dial :waste @17:46:38.38 CS.reconfigure :default @17:46:38.44 CS.seek 0ml @17:46:58.43 Flushing lower manifold's waste1 port @17:46:58.57 CSV.dial :flush @17:46:59.01 CS.reconfigure :fast @17:46:59.08 CS.seek 5ml @17:47:11.83 delay 5 seconds @17:47:16.85 CSV.dial :air @17:47:17.36 CS.seek 7.001ml @17:47:22.86 CSV.dial CTV @17:47:22.90
CTV.dial CSV @17:47:24.30 CBV.dial :waste1 @17:47:25.09 CS.seek 0ml @17:47:41.61 Flushing lower manifold's waste2 port @17:47:41.75 CSV.dial :flush @17:47:42.48 CS.seek 5ml @17:47:55.27 delay 5 seconds @17:48:00.29 CSV.dial :air @17:48:00.81 CS.seek 7ml @17:48:06.30 CSV.dial CTV @17:48:06.32
CTV.dial CSV @17:48:06.79 CBV.dial :waste2 @17:48:07.85 CS.seek 0ml @17:48:24.55 Flushing lower manifold's waste2b port @17:48:24.68 CSV.dial :flush @17:48:25.43 CS.seek 5ml @17:48:38.02 delay 5 seconds @17:48:43.04 CSV.dial :air @17:48:43.57 CS.seek 7ml @17:48:49.21 CSV.dial CTV @17:48:49.24
CTV.dial CSV @17:48:49.69 CBV.dial :waste2b @17:48:50.74 CS.seek 0ml @17:49:07.17 Disinfecting external Intake with kill @17:49:07.30 CSV.dial CSR @17:49:07.81 Pulling 8ml of CSR.kill @17:49:07.82 CSR.select :kill @17:49:07.96 CS.seek 8ml @17:49:27.61 delay 5 seconds @17:49:32.63 CSR.select :air @17:49:32.76 CS.seek 10ml @17:49:38.37 CSV.dial CBV @17:49:38.39
CBV.dial CSV @17:49:39.47 CTV.dial :intake @17:49:40.27 Intake.select :open @17:49:40.39 CS.seek 2ml @17:49:59.11 Intake.select :close @17:49:59.22 CTV.dial :air @17:50:00.00 CS.reconfigure :default @17:50:00.06 CS.seek 0ml @17:50:06.05 CSV.dial CSR @17:50:07.12 Pulling 5ml of CSR.flush CSR.select :flush @17:50:07.26 CS.seek 5ml @17:50:20.86 delay 5 seconds @17:50:25.88 CSR.select :air @17:50:26.01 CS.seek 10ml @17:50:39.58 CSV.dial CBV @17:50:40.59 CS.seek 0ml @17:51:07.08 Garaging FlushPuck in Collection Clamp @17:51:07.16 Elbow.reconfigure :tight @17:51:07.22 Elbow.seek CC @17:51:07.53 Elbow.reconfigure :loose @17:51:07.64 Forearm.seek CC @17:51:08.66 Hand.close @17:51:09.29 CC.seek :open @17:51:13.90 Forearm.seek :retract @17:51:14.87 Elbow.reconfigure :tight @17:51:14.93 Elbow.seek :garage @17:51:17.96 Elbow.reconfigure :loose @17:51:18.06 Forearm.reconfigure :tight @17:51:18.12 Forearm.seek :garage @17:51:19.55 Hand.open @17:51:20.22 Forearm.reconfigure :loose @17:51:20.28 Forearm.seek :retract @17:51:21.24 Completed [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] begun 14:44:40 @17:51:21.31 Asleep after completing DA_WCR_021423 assays @17:51:21.33 GoodNews.email "{1 => 33,\n 2 => 33,\n 3 => 2,\n 4 => 31,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@17:43:49, 17% flow, 20.8C, 33% humidity, 14.8psia, 13.739V, 0.429A, 0.411A avg, 5.90W\nSampled 775.0ml",Subject: "Asleep after completing DA_WCR_021423 assays" @17:51:26.40 upsync @17:51:26.49 Session Ended @13:54:01.58UTC21-Feb-23
ESP2 v3.41 I2C Gateway v3.20 for core bus @13:54:04.26 Configuration for short puck jake 1/11/21 by brent@mbari.org /home/esp/esp2/type/shallow/jake/configure.rb @13:54:08.23 Polling Can every 10:00 @13:54:08.42 Cleanly exited at 13:49:45UTC21-Feb-23 @13:54:08.46 Loading /home/esp/esp2/mission/DA_WCR_022123.rb mission @13:54:08.52 Preparing to sleep until + 00:00:05 @13:54:08.53 GoodNews.email "Starting DA_WCR_022123 w/pucks in tube 4\n{1 => 33,\n 2 => 33,\n 3 => 2,\n 4 => 31,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@13:54:08, 16% flow, 18.4C, 38% humidity, 14.7psia, 13.749V, 0.345A, 0.327A avg, 4.74W",Subject: "DA_WCR_022123 begun" @13:54:08.57
Asleep til + 00:00:05 @13:54:08.61 Continuing at 13:54 @13:54:08.85 Gate.power :core,:OFF @13:54:08.87 Gate.power :raw,:OFF Shutdown @13:54:12.84 GoodNews.email "Then, in tube 4: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2/mission/DA_WCR_022123.rb:14\n{1 => 33,\n 2 => 33,\n 3 => 2,\n 4 => 31,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@13:54:08, 16% flow, 18.4C, 38% humidity, 14.7psia, 13.749V, 0.345A, 0.327A avg, 4.74W",Subject: "Asleep until 13:54" @13:54:13.49
Powering core bus @13:54:14.64 upsync @13:54:18.09 upsync @13:54:18.89
Gate.power :raw,:ON @13:54:19.87 CS.configure :default @13:54:19.96 PS.configure :default @13:54:20.05 SS.configure :default @13:54:20.14 CC.configure :default @13:54:20.23 PC.configure :default @13:54:20.33 Forearm.configure :loose @13:54:20.41 Elbow.configure :loose @13:54:20.52 SC.configure :loose @13:54:20.61 SE.configure :default @13:54:20.65 CH.coast @13:54:20.70 CH.configure :default @13:54:20.72 PH.coast @13:54:20.78 PH.configure :default @13:54:20.81 All dwarf controllers running firmware version 3.85 @13:54:20.91 Valve.reset @13:54:21.16 Intake.select :close @13:54:21.20 Exhaust.select :close @13:54:21.21 CTV.dial :air @13:54:21.29 PSV.dial :air @13:54:21.36 CSV.dial :waste1 @13:54:21.43
Forearm.reconfigure :loose @13:54:21.48 Forearm.home.seek :retract @13:54:21.82 SSV.dial :spit,avoiding: :exhaust @13:54:22.20 Hand.close @13:54:22.23
Elbow.jog 1500 @13:54:22.39 PS.reconfigure :default @13:54:22.43 PS.home.seek 0ml @13:54:22.64 CS.reconfigure :fast @13:54:22.67 SS.reconfigure :default @13:54:22.76 CS.home.seek 0ml @13:54:22.78 SS.home.seek 0ml @13:54:22.90
Elbow.reconfigure :loose @13:54:22.94 Elbow.home.seek :clear @13:54:26.21 SE.reconfigure :default @13:54:26.25 SE.home.seek :down @13:54:26.87 SC.reconfigure :tight @13:54:26.92 SC.home.seek :initial @13:54:30.71 PS.stop @13:54:55.25
SC.configure :limp @13:54:55.30 Forearm.seek :retract @13:54:55.35 Elbow.seek :hammer @13:54:56.37 Forearm.reconfigure :tight @13:54:56.41 Forearm.seek retracted @13:54:56.63 CC.reconfigure :default @13:54:56.64 PC.reconfigure :default @13:54:56.73
CC.home.seek :open @13:54:56.75 PC.home.seek :open @13:55:00.24 Notice.email "At 13:55:00 began in tube 4: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2/mission/DA_WCR_022123.rb:14\n{1 => 33,\n 2 => 33,\n 3 => 2,\n 4 => 31,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@13:55:00, 16% flow, 18.4C, 38% humidity, 14.7psia, 13.754V, 0.327A, 0.309A avg, 4.49W",Subject: "Started DA_WCR_022123 phase initialPurge; da [1000, 1000] #da and wsr are run simultanelously" @13:55:00.27
Phase [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] @13:55:00.28 Starting initialPurge @13:55:00.30 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @13:55:00.36 Hand.open @13:55:01.07 Elbow.reconfigure :tight @13:55:01.12 Elbow.seek FlushPuck::Garage @13:55:02.96 Elbow.reconfigure :loose @13:55:03.05 Forearm.reconfigure :loose @13:55:03.09 Forearm.seek :garage @13:55:04.05 Hand.close @13:55:04.81 Forearm.seek :retract @13:55:05.07 upsync @13:55:05.78
Elbow.reconfigure :tight @13:55:05.82 Elbow.seek CC @13:55:08.68 Elbow.reconfigure :loose @13:55:08.77 Forearm.reconfigure :tight @13:55:08.81 Forearm.seek CC @13:55:10.10 CC.seek :closed @13:55:14.63 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.33 @13:55:14.70 Hand.open @13:55:15.37 Forearm.reconfigure :loose @13:55:15.41 Forearm.seek :retract @13:55:16.37 Flushing the Collection Valve::Manifold @13:55:16.49 CSV.dial CSR @13:55:17.81 Pulling 5ml of CSR.flush @13:55:17.82 CSR.select :flush @13:55:17.93 CS.reconfigure :default @13:55:17.97 CS.seek 5ml @13:55:31.57 delay 5 seconds @13:55:36.59 CSR.select :air @13:55:36.73 CS.reconfigure :fast @13:55:36.77 CS.seek 7.5ml @13:55:43.50 CSV.dial :waste @13:55:44.79 CS.reconfigure :default @13:55:44.83 CS.seek 0ml @13:56:04.82 Purging external Intake @13:56:04.93 CSV.dial :flush @13:56:05.37 CS.reconfigure :fast @13:56:05.46 CS.seek 10ml @13:56:29.66 delay 5 seconds @13:56:34.68 CSV.dial CBV @13:56:34.71
CBV.dial CSV @13:56:35.46 CTV.dial :intake @13:56:36.24 Intake.select :open @13:56:36.34 CS.seek 0ml @13:56:59.78 Intake.select :close @13:56:59.88 CTV.dial :air @13:57:00.68 Flushing upper manifold @13:57:00.79 CSV.dial :flush @13:57:01.52 CS.seek 5ml @13:57:14.10 delay 5 seconds @13:57:19.12 CSV.dial :air @13:57:19.61 CS.seek 6.999ml @13:57:25.16 CSV.dial CBV @13:57:25.18
CBV.dial CSV @13:57:26.21 CTV.dial :air @13:57:26.22 CS.seek 0ml @13:57:42.81 delay 7 seconds @13:57:49.84 CS.seek 3ml @13:57:57.84 CSV.dial :waste @13:57:58.29 CS.seek 0ml @13:58:05.98 CSV.dial :flush @13:58:06.41 CS.seek 3ml @13:58:14.35 delay 5 seconds @13:58:19.36 CSV.dial :air @13:58:19.86 CS.seek 5ml @13:58:25.53 CSV.dial :air @13:58:25.55 CS.seek 0ml @13:58:37.69 delay 7 seconds @13:58:44.71 CS.seek 3ml @13:58:52.69 CSV.dial :waste @13:58:53.41 CS.seek 0ml @13:59:01.22 Garaging FlushPuck in Collection Clamp @13:59:01.29 Elbow.reconfigure :tight @13:59:01.33 Elbow.seek CC @13:59:01.44 Elbow.reconfigure :loose @13:59:01.52 Forearm.seek CC @13:59:02.47 Hand.close @13:59:03.06 CC.seek :open @13:59:07.67 Forearm.seek :retract @13:59:08.68 Elbow.reconfigure :tight @13:59:08.72 Elbow.seek :garage @13:59:11.70 Elbow.reconfigure :loose @13:59:11.79 Forearm.reconfigure :tight @13:59:11.83 Forearm.seek :garage @13:59:12.78 Hand.open @13:59:13.93 Forearm.reconfigure :loose @13:59:13.97 Forearm.seek :retract @13:59:14.92 da sampling at most 1000ml, wcr at most 1000ml @13:59:14.95 Loading sh1 puck from tube 4 into Collection Clamp @13:59:14.96 DAfans.email "Started da in tube 4 at 13:59:14UTC21-Feb-23\n{1 => 33,\n 2 => 33,\n 3 => 2,\n 4 => 31,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@13:55:00, 16% flow, 18.4C, 38% humidity, 14.7psia, 13.754V, 0.327A, 0.309A avg, 4.49W",Subject: "da sampling at most 1000ml, wcr at most 1000ml" @13:59:15.44
Elbow.reconfigure :tight @13:59:15.49 Elbow.seek 4 @13:59:17.42 Forearm.reconfigure :tight @13:59:17.46 Forearm.seek 4 + 450 counts @13:59:18.67 SE.reconfigure :pick @13:59:18.78 SE.seek :beamBreak @13:59:19.87 SE.configure :default @13:59:20.02 Elbow.seek 4 @13:59:20.21 Forearm.seek 4 + 300 counts @13:59:20.30 upsync @13:59:20.44
Forearm.reconfigure :loose @13:59:20.49 Hand.close @13:59:21.07 SE.seek :down @13:59:21.26
Forearm.seek :retract @13:59:21.80 SE.stop @13:59:22.20
Elbow.seek CC @13:59:23.85 Elbow.reconfigure :loose @13:59:23.94 Forearm.reconfigure :tight @13:59:23.98 Forearm.seek CC @13:59:25.23 CC.seek :closed @13:59:29.73 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.20 @13:59:29.74 Hand.open @13:59:30.36 Forearm.reconfigure :loose @13:59:30.40 Forearm.seek :retract @13:59:31.35 collectSample 1000ml w/bubble point of 24psi @13:59:31.36 Priming 7ml @13:59:31.37 Intake.select :close @13:59:31.38 Exhaust.select :close @13:59:31.39 SS.hold @13:59:31.59 Calibrating pressure and temperature sensors @13:59:31.61 CTV.dial :air @13:59:31.63 SSV.dial :spit,avoiding: :exhaust @13:59:31.65 SS.reconfigure :fast @13:59:31.69 SS.seek 5ml @13:59:34.56 SSV.dial CBV @13:59:34.58
CBV.dial SSV @13:59:35.90 delay 3.8 seconds @13:59:39.82 SSV.dial :spit @13:59:41.11 SS.seek 0ml @13:59:43.87 SSV.dial CBV @13:59:45.17 CTV.dial :intake @13:59:45.97 SSV.dial CBV @13:59:45.99
CBV.dial SSV @13:59:46.01 Allowing 40s for 7.0ml of remaining 7.0ml @13:59:46.02 Intake.select :open @13:59:48.17 Intake @15.1psia, vacuum from 9.6 to 12.6psi @13:59:48.19 SS.reconfigure :inhaling 2.20ml/s @13:59:48.23 SS.seek 7ml @13:59:54.76 Stroke completed -- waiting for pressure to equalize ... @13:59:54.77 SS.reconfigure :exhale @13:59:54.81 SS.hold @13:59:58.25 Intake.select :close @13:59:58.39 SSV.dial :exhaust @13:59:59.15 Exhaust.select :open @13:59:59.26 SS.seek 0ml @14:00:03.06 Exhaust.select :close @14:00:03.16 Sampling 1000.0ml @14:00:03.17 Intake.select :close @14:00:03.18 Exhaust.select :close @14:00:03.19 SS.reconfigure :default @14:00:03.23 SS.hold @14:00:03.42 CTV.dial :intake @14:00:03.43 SSV.dial CBV @14:00:03.45
CBV.dial SSV @14:00:04.22 Allowing 130s for 25.0ml of remaining 1000.0ml @14:00:04.23 Intake.select :open @14:00:06.38 Intake @15.5psia, vacuum from 10.0 to 13.0psi @14:00:06.39 SS.reconfigure :inhaling 2.20ml/s @14:00:06.43 SS.seek 25ml @14:00:15.16 Vacuum too high -- waiting up to 40 seconds @14:00:15.17 SS.reconfigure :exhale @14:00:15.21 SS.hold @14:00:20.87 Intake @15.7psia, vacuum from 10.0 to 13.0psi @14:00:20.88 SS.reconfigure :inhaling 1.98ml/s @14:00:20.93 SS.seek 25ml @14:00:26.35 Vacuum too high -- waiting up to 40 seconds @14:00:26.36 SS.reconfigure :exhale @14:00:26.40 SS.hold @14:00:33.15 Intake @16.2psia, vacuum from 10.0 to 13.0psi @14:00:33.17 SS.reconfigure :inhaling 1.79ml/s @14:00:33.21 SS.seek 25ml @14:00:36.51 Stroke completed -- waiting for pressure to equalize ... @14:00:36.52 SS.reconfigure :exhale @14:00:36.56 SS.hold @14:00:42.09 Intake.select :close @14:00:42.23 SSV.dial :exhaust @14:00:42.96 Exhaust.select :open @14:00:43.07 SS.seek 0ml @14:00:51.68 Exhaust.select :close @14:00:51.78 SSV.dial CBV @14:00:51.81
CBV.dial SSV @14:00:52.56 Allowing 130s for 25.0ml of remaining 975.0ml @14:00:52.57 Intake.select :open @14:00:54.72 Intake @16.1psia, vacuum from 10.0 to 13.0psi @14:00:54.74 SS.reconfigure :inhaling 1.79ml/s @14:00:54.78 SS.seek 25ml @14:01:01.97 Vacuum too high -- waiting up to 40 seconds @14:01:01.98 SS.reconfigure :exhale @14:01:02.02 SS.hold @14:01:07.06 Intake @16.1psia, vacuum from 10.0 to 13.0psi @14:01:07.08 SS.reconfigure :inhaling 1.61ml/s @14:01:07.12 SS.seek 25ml @14:01:12.23 Vacuum too high -- waiting up to 40 seconds @14:01:12.24 SS.reconfigure :exhale @14:01:12.28 SS.hold @14:01:18.04 Intake @16.3psia, vacuum from 10.0 to 13.0psi @14:01:18.05 SS.reconfigure :inhaling 1.45ml/s @14:01:18.10 SS.seek 25ml @14:01:22.56 Vacuum too high -- waiting up to 40 seconds @14:01:22.57 SS.reconfigure :exhale @14:01:22.61 SS.hold @14:01:27.59 Intake @16.4psia, vacuum from 10.0 to 13.0psi @14:01:27.60 SS.reconfigure :inhaling 1.30ml/s @14:01:27.65 SS.seek 25ml @14:01:31.87 Stroke completed -- waiting for pressure to equalize ... @14:01:31.88 SS.reconfigure :exhale @14:01:31.92 SS.hold @14:01:40.43 Intake.select :close @14:01:40.57 SSV.dial :exhaust @14:01:41.31 Exhaust.select :open @14:01:41.42 SS.seek 0ml @14:01:50.43 Exhaust.select :close @14:01:50.53 SSV.dial CBV @14:01:50.55
CBV.dial SSV @14:01:51.81 Allowing 130s for 25.0ml of remaining 950.0ml @14:01:51.82 Intake.select :open @14:01:53.97 Intake @16.1psia, vacuum from 10.0 to 13.0psi @14:01:53.99 SS.reconfigure :inhaling 1.30ml/s @14:01:54.03 SS.seek 25ml @14:02:02.66 Vacuum too high -- waiting up to 40 seconds @14:02:02.67 SS.reconfigure :exhale @14:02:02.71 SS.hold @14:02:07.61 Intake @16.0psia, vacuum from 10.0 to 13.0psi @14:02:07.62 SS.reconfigure :inhaling 1.17ml/s @14:02:07.67 SS.seek 25ml @14:02:15.59 Vacuum too high -- waiting up to 40 seconds @14:02:15.60 SS.reconfigure :exhale @14:02:15.64 SS.hold @14:02:22.18 Intake @16.3psia, vacuum from 10.0 to 13.0psi @14:02:22.19 SS.reconfigure :inhaling 1.06ml/s @14:02:22.24 SS.seek 25ml @14:02:29.75 Vacuum too high -- waiting up to 40 seconds @14:02:29.76 SS.reconfigure :exhale @14:02:29.80 SS.hold @14:02:36.29 Intake @16.6psia, vacuum from 10.0 to 13.0psi @14:02:36.30 SS.reconfigure :inhaling 0.95ml/s @14:02:36.35 SS.seek 25ml @14:02:37.26 Stroke completed -- waiting for pressure to equalize ... @14:02:37.27 SS.reconfigure :exhale @14:02:37.31 SS.hold @14:02:41.39 Intake.select :close @14:02:41.53 SSV.dial :exhaust @14:02:42.29 Exhaust.select :open @14:02:42.40 SS.seek 0ml @14:02:51.13 Exhaust.select :close @14:02:51.24 SSV.dial CBV @14:02:51.27
CBV.dial SSV @14:02:52.03 Allowing 130s for 25.0ml of remaining 925.0ml Intake.select :open @14:02:54.19 Intake @16.2psia, vacuum from 10.0 to 13.0psi @14:02:54.21 SS.reconfigure :inhaling 0.95ml/s @14:02:54.25 SS.seek 25ml @14:03:07.03 Vacuum too high -- waiting up to 40 seconds @14:03:07.04 SS.reconfigure :exhale @14:03:07.08 SS.hold @14:03:11.92 Intake @16.0psia, vacuum from 10.0 to 13.0psi @14:03:11.93 SS.reconfigure :inhaling 0.87ml/s @14:03:11.98 SS.seek 25ml @14:03:24.12 Vacuum too high -- waiting up to 40 seconds @14:03:24.13 SS.reconfigure :exhale @14:03:24.17 SS.hold @14:03:30.65 Intake @16.2psia, vacuum from 10.0 to 13.0psi @14:03:30.66 SS.reconfigure :inhaling 0.78ml/s @14:03:30.70 SS.seek 25ml @14:03:37.49 Stroke completed -- waiting for pressure to equalize ... @14:03:37.50 SS.reconfigure :exhale @14:03:37.54 SS.hold @14:03:45.99 Intake.select :close @14:03:46.12 SSV.dial :exhaust @14:03:46.87 Exhaust.select :open @14:03:46.97 SS.seek 0ml @14:03:55.94 Exhaust.select :close @14:03:56.05 SSV.dial CBV @14:03:56.08
CBV.dial SSV @14:03:56.82 Allowing 130s for 25.0ml of remaining 900.0ml @14:03:56.83 Intake.select :open @14:03:58.98 Intake @16.1psia, vacuum from 10.0 to 13.0psi @14:03:59.00 SS.reconfigure :inhaling 0.78ml/s @14:03:59.04 SS.seek 25ml @14:04:20.06 Vacuum too high -- waiting up to 40 seconds @14:04:20.07 SS.reconfigure :exhale @14:04:20.11 SS.hold @14:04:26.66 Intake @16.2psia, vacuum from 10.0 to 13.0psi @14:04:26.67 SS.reconfigure :inhaling 0.70ml/s @14:04:26.71 SS.seek 25ml @14:04:41.46 Stroke completed -- waiting for pressure to equalize ... @14:04:41.47 SS.reconfigure :exhale @14:04:41.51 SS.hold @14:04:52.51 Intake.select :close @14:04:52.65 SSV.dial :exhaust @14:04:53.40 Exhaust.select :open @14:04:53.50 SS.seek 0ml @14:05:02.22 Exhaust.select :close @14:05:02.33 SSV.dial CBV @14:05:02.37
CBV.dial SSV @14:05:03.09 Allowing 130s for 25.0ml of remaining 875.0ml @14:05:03.10 Intake.select :open @14:05:05.25 Intake @16.1psia, vacuum from 10.0 to 13.0psi @14:05:05.26 SS.reconfigure :inhaling 0.70ml/s @14:05:05.31 SS.seek 25ml @14:05:27.78 Vacuum too high -- waiting up to 40 seconds @14:05:27.79 SS.reconfigure :exhale @14:05:27.83 SS.hold @14:05:33.44 Intake @15.8psia, vacuum from 10.0 to 13.0psi @14:05:33.45 SS.reconfigure :inhaling 0.63ml/s @14:05:33.50 SS.seek 25ml @14:05:50.99 Stroke completed -- waiting for pressure to equalize ... @14:05:51.00 SS.reconfigure :exhale @14:05:51.04 SS.hold @14:06:03.60 Intake.select :close @14:06:03.74 SSV.dial :exhaust @14:06:04.49 Exhaust.select :open @14:06:04.60 SS.seek 0ml @14:06:13.61 Exhaust.select :close @14:06:13.71 SSV.dial CBV @14:06:13.74
CBV.dial SSV @14:06:14.51 Allowing 130s for 25.0ml of remaining 850.0ml @14:06:14.52 Intake.select :open @14:06:16.67 Intake @16.1psia, vacuum from 10.0 to 13.0psi @14:06:16.68 SS.reconfigure :inhaling 0.63ml/s @14:06:16.72 SS.seek 25ml @14:06:54.71 Vacuum too high -- waiting up to 40 seconds @14:06:54.72 SS.reconfigure :exhale @14:06:54.76 SS.hold @14:07:02.20 Intake @16.0psia, vacuum from 10.0 to 13.0psi @14:07:02.21 SS.reconfigure :inhaling 0.57ml/s @14:07:02.26 SS.seek 25ml @14:07:06.89 Stroke completed -- waiting for pressure to equalize ... @14:07:06.90 SS.reconfigure :exhale @14:07:06.94 SS.hold @14:07:15.39 Intake.select :close @14:07:15.53 SSV.dial :exhaust @14:07:16.28 Exhaust.select :open @14:07:16.39 SS.seek 0ml @14:07:25.33 Exhaust.select :close @14:07:25.43 SSV.dial CBV @14:07:25.45
CBV.dial SSV @14:07:26.22 Allowing 130s for 25.0ml of remaining 825.0ml @14:07:26.23 Intake.select :open @14:07:28.38 Intake @16.0psia, vacuum from 10.0 to 13.0psi @14:07:28.40 SS.reconfigure :inhaling 0.57ml/s @14:07:28.44 SS.seek 25ml @14:08:13.88 Stroke completed -- waiting for pressure to equalize ... @14:08:13.89 SS.reconfigure :exhale @14:08:13.93 SS.hold @14:08:28.20 Intake.select :close @14:08:28.33 SSV.dial :exhaust @14:08:29.10 Exhaust.select :open @14:08:29.20 SS.seek 0ml @14:08:38.02 Exhaust.select :close @14:08:38.12 SSV.dial CBV @14:08:38.14
CBV.dial SSV @14:08:38.91 Allowing 130s for 25.0ml of remaining 800.0ml @14:08:38.92 Intake.select :open @14:08:41.07 Intake @16.1psia, vacuum from 10.0 to 13.0psi @14:08:41.08 SS.reconfigure :inhaling 0.57ml/s @14:08:41.13 SS.seek 25ml @14:09:27.36 Stroke completed -- waiting for pressure to equalize ... @14:09:27.37 SS.reconfigure :exhale @14:09:27.41 SS.hold @14:09:43.57 Intake.select :close @14:09:43.71 SSV.dial :exhaust @14:09:44.47 Exhaust.select :open @14:09:44.57 SS.seek 0ml @14:09:53.26 Exhaust.select :close @14:09:53.37 SSV.dial CBV @14:09:53.42
CBV.dial SSV @14:09:54.14 Allowing 130s for 25.0ml of remaining 775.0ml @14:09:54.15 Intake.select :open @14:09:56.30 Intake @16.0psia, vacuum from 10.0 to 13.0psi @14:09:56.32 SS.reconfigure :inhaling 0.57ml/s @14:09:56.36 SS.seek 25ml @14:10:36.27 Vacuum too high -- waiting up to 40 seconds @14:10:36.28 SS.reconfigure :exhale @14:10:36.32 SS.hold @14:10:44.84 Intake @15.9psia, vacuum from 10.0 to 13.0psi @14:10:44.85 SS.reconfigure :inhaling 0.51ml/s @14:10:44.90 SS.seek 25ml @14:10:52.06 Stroke completed -- waiting for pressure to equalize ... @14:10:52.07 SS.reconfigure :exhale @14:10:52.11 SS.hold @14:11:02.38 Intake.select :close @14:11:02.52 SSV.dial :exhaust @14:11:03.28 Exhaust.select :open @14:11:03.39 SS.seek 0ml @14:11:12.43 Exhaust.select :close @14:11:12.54 SSV.dial CBV @14:11:12.56
CBV.dial SSV @14:11:13.31 Allowing 130s for 25.0ml of remaining 750.0ml @14:11:13.32 Intake.select :open @14:11:15.47 Intake @16.0psia, vacuum from 10.0 to 13.0psi @14:11:15.49 SS.reconfigure :inhaling 0.51ml/s @14:11:15.53 SS.seek 25ml @14:12:06.03 Stroke completed -- waiting for pressure to equalize ... @14:12:06.04 SS.reconfigure :exhale @14:12:06.08 SS.hold @14:12:22.30 Intake.select :close @14:12:22.43 SSV.dial :exhaust @14:12:23.17 Exhaust.select :open @14:12:23.27 SS.seek 0ml @14:12:32.29 Exhaust.select :close @14:12:32.39 SSV.dial CBV @14:12:32.42
CBV.dial SSV @14:12:33.17 Allowing 130s for 25.0ml of remaining 725.0ml @14:12:33.18 Intake.select :open @14:12:35.33 Intake @16.1psia, vacuum from 10.0 to 13.0psi @14:12:35.35 SS.reconfigure :inhaling 0.51ml/s @14:12:35.39 SS.seek 25ml @14:13:26.28 Stroke completed -- waiting for pressure to equalize ... @14:13:26.29 SS.reconfigure :exhale @14:13:26.33 SS.hold @14:13:44.52 Intake.select :close @14:13:44.65 SSV.dial :exhaust @14:13:45.39 Exhaust.select :open @14:13:45.49 SS.seek 0ml @14:13:54.49 Exhaust.select :close @14:13:54.60 SSV.dial CBV @14:13:54.64
CBV.dial SSV @14:13:55.36 Allowing 130s for 25.0ml of remaining 700.0ml @14:13:55.37 Intake.select :open @14:13:57.52 Intake @16.0psia, vacuum from 10.0 to 13.0psi @14:13:57.53 SS.reconfigure :inhaling 0.51ml/s @14:13:57.58 SS.seek 25ml @14:14:47.73 Stroke completed -- waiting for pressure to equalize ... @14:14:47.74 SS.reconfigure :exhale @14:14:47.78 SS.hold @14:15:06.08 Intake.select :close @14:15:06.21 SSV.dial :exhaust @14:15:06.95 Exhaust.select :open @14:15:07.05 SS.seek 0ml @14:15:16.09 Exhaust.select :close @14:15:16.19 SSV.dial CBV @14:15:16.21
CBV.dial SSV @14:15:16.96 Allowing 130s for 25.0ml of remaining 675.0ml @14:15:16.97 Intake.select :open @14:15:19.13 Intake @16.1psia, vacuum from 10.0 to 13.0psi @14:15:19.14 SS.reconfigure :inhaling 0.51ml/s @14:15:19.18 SS.seek 25ml @14:16:01.17 Vacuum too high -- waiting up to 40 seconds @14:16:01.18 SS.reconfigure :exhale @14:16:01.22 SS.hold @14:16:09.79 Intake @16.0psia, vacuum from 10.0 to 13.0psi @14:16:09.81 SS.reconfigure :inhaling 0.47ml/s @14:16:09.85 SS.seek 25ml @14:16:19.71 Stroke completed -- waiting for pressure to equalize ... @14:16:19.72 SS.reconfigure :exhale @14:16:19.76 SS.hold @14:16:32.42 Intake.select :close @14:16:32.56 SSV.dial :exhaust @14:16:33.30 Exhaust.select :open @14:16:33.41 SS.seek 0ml @14:16:42.21 Exhaust.select :close @14:16:42.31 SSV.dial CBV @14:16:42.33
CBV.dial SSV @14:16:43.09 Allowing 130s for 25.0ml of remaining 650.0ml @14:16:43.10 Intake.select :open @14:16:45.25 Intake @16.0psia, vacuum from 10.0 to 13.0psi @14:16:45.26 SS.reconfigure :inhaling 0.47ml/s @14:16:45.31 SS.seek 25ml @14:17:41.25 Stroke completed -- waiting for pressure to equalize ... @14:17:41.26 SS.reconfigure :exhale @14:17:41.31 SS.hold @14:18:01.56 Intake.select :close @14:18:01.69 SSV.dial :exhaust @14:18:02.43 Exhaust.select :open @14:18:02.54 SS.seek 0ml @14:18:11.52 Exhaust.select :close @14:18:11.62 SSV.dial CBV @14:18:11.64
CBV.dial SSV @14:18:12.40 Allowing 130s for 25.0ml of remaining 625.0ml @14:18:12.41 Intake.select :open @14:18:14.56 Intake @16.0psia, vacuum from 10.0 to 13.0psi @14:18:14.57 SS.reconfigure :inhaling 0.47ml/s @14:18:14.62 SS.seek 25ml @14:19:10.19 Stroke completed -- waiting for pressure to equalize ... @14:19:10.20 SS.reconfigure :exhale @14:19:10.29 SS.hold @14:19:30.62 Intake.select :close @14:19:30.76 SSV.dial :exhaust @14:19:31.50 Exhaust.select :open @14:19:31.61 SS.seek 0ml @14:19:40.39 Exhaust.select :close @14:19:40.50 SSV.dial CBV @14:19:40.54
CBV.dial SSV @14:19:41.25 Allowing 130s for 25.0ml of remaining 600.0ml @14:19:41.26 Intake.select :open @14:19:43.42 Intake @16.0psia, vacuum from 10.0 to 13.0psi @14:19:43.43 SS.reconfigure :inhaling 0.47ml/s @14:19:43.48 SS.seek 25ml @14:20:36.17 Vacuum too high -- waiting up to 40 seconds SS.reconfigure :exhale @14:20:36.21 SS.hold @14:20:49.31 Intake @16.6psia, vacuum from 10.0 to 13.0psi @14:20:49.33 SS.reconfigure :inhaling 0.43ml/s @14:20:49.37 SS.seek 25ml @14:20:53.15 Stroke completed -- waiting for pressure to equalize ... @14:20:53.16 SS.reconfigure :exhale @14:20:53.20 SS.hold @14:21:04.21 Intake.select :close @14:21:04.34 SSV.dial :exhaust @14:21:05.08 Exhaust.select :open @14:21:05.19 SS.seek 0ml @14:21:13.95 Exhaust.select :close @14:21:14.06 SSV.dial CBV @14:21:14.11
CBV.dial SSV @14:21:14.82 Allowing 130s for 25.0ml of remaining 575.0ml @14:21:14.83 Intake.select :open @14:21:16.98 Intake @16.1psia, vacuum from 10.0 to 13.0psi @14:21:16.99 SS.reconfigure :inhaling 0.43ml/s @14:21:17.04 SS.seek 25ml @14:22:19.05 Stroke completed -- waiting for pressure to equalize ... @14:22:19.06 SS.reconfigure :exhale @14:22:19.10 SS.hold @14:22:41.38 Intake.select :close @14:22:41.52 SSV.dial :exhaust @14:22:42.27 Exhaust.select :open @14:22:42.37 SS.seek 0ml @14:22:51.37 Exhaust.select :close @14:22:51.48 SSV.dial CBV @14:22:51.53
CBV.dial SSV @14:22:52.24 Allowing 130s for 25.0ml of remaining 550.0ml @14:22:52.25 Intake.select :open @14:22:54.41 Intake @16.1psia, vacuum from 10.0 to 13.0psi @14:22:54.42 SS.reconfigure :inhaling 0.43ml/s @14:22:54.46 SS.seek 25ml @14:23:55.61 Stroke completed -- waiting for pressure to equalize ... @14:23:55.62 SS.reconfigure :exhale @14:23:55.66 SS.hold @14:24:17.94 Intake.select :close @14:24:18.08 SSV.dial :exhaust @14:24:18.82 Exhaust.select :open @14:24:18.93 SS.seek 0ml @14:24:27.72 Exhaust.select :close @14:24:27.82 SSV.dial CBV @14:24:27.84
CBV.dial SSV @14:24:28.59 Allowing 130s for 25.0ml of remaining 525.0ml @14:24:28.60 Intake.select :open @14:24:30.76 Intake @16.3psia, vacuum from 10.0 to 13.0psi @14:24:30.78 SS.reconfigure :inhaling 0.43ml/s @14:24:30.82 SS.seek 25ml @14:25:18.85 Vacuum too high -- waiting up to 40 seconds @14:25:18.86 SS.reconfigure :exhale @14:25:18.90 SS.hold @14:25:30.02 Intake @16.7psia, vacuum from 10.0 to 13.0psi @14:25:30.03 SS.reconfigure :inhaling 0.38ml/s @14:25:30.08 SS.seek 25ml @14:25:45.84 Stroke completed -- waiting for pressure to equalize ... @14:25:45.85 SS.reconfigure :exhale @14:25:45.89 SS.hold @14:26:02.05 Intake.select :close @14:26:02.19 SSV.dial :exhaust @14:26:02.93 Exhaust.select :open @14:26:03.04 SS.seek 0ml @14:26:12.03 Exhaust.select :close @14:26:12.13 SSV.dial CBV @14:26:12.15
CBV.dial SSV @14:26:12.92 Allowing 130s for 25.0ml of remaining 500.0ml @14:26:12.93 Intake.select :open @14:26:15.08 Intake @16.2psia, vacuum from 10.0 to 13.0psi @14:26:15.10 SS.reconfigure :inhaling 0.38ml/s @14:26:15.14 SS.seek 25ml @14:27:24.63 Stroke completed -- waiting for pressure to equalize ... @14:27:24.64 SS.reconfigure :exhale @14:27:24.68 SS.hold @14:27:44.96 Intake.select :close @14:27:45.10 SSV.dial :exhaust @14:27:45.86 Exhaust.select :open @14:27:45.97 SS.seek 0ml @14:27:54.84 Exhaust.select :close @14:27:54.94 SSV.dial CBV @14:27:54.96
CBV.dial SSV @14:27:55.73 Allowing 130s for 25.0ml of remaining 475.0ml @14:27:55.74 Intake.select :open @14:27:57.89 Intake @16.1psia, vacuum from 10.0 to 13.0psi @14:27:57.91 SS.reconfigure :inhaling 0.38ml/s @14:27:57.95 SS.seek 25ml @14:29:08.29 Stroke completed -- waiting for pressure to equalize ... @14:29:08.30 SS.reconfigure :exhale @14:29:08.34 SS.hold @14:29:31.08 Intake.select :close @14:29:31.22 SSV.dial :exhaust @14:29:31.98 Exhaust.select :open @14:29:32.08 SS.seek 0ml @14:29:40.91 Exhaust.select :close @14:29:41.02 SSV.dial CBV @14:29:41.06
CBV.dial SSV @14:29:41.79 Allowing 130s for 25.0ml of remaining 450.0ml @14:29:41.80 Intake.select :open @14:29:43.95 Intake @16.2psia, vacuum from 10.0 to 13.0psi @14:29:43.97 SS.reconfigure :inhaling 0.38ml/s @14:29:44.01 SS.seek 25ml @14:30:54.22 Stroke completed -- waiting for pressure to equalize ... @14:30:54.23 SS.reconfigure :exhale @14:30:54.27 SS.hold @14:31:18.59 Intake.select :close @14:31:18.73 SSV.dial :exhaust @14:31:19.49 Exhaust.select :open @14:31:19.60 SS.seek 0ml @14:31:28.46 Exhaust.select :close @14:31:28.56 SSV.dial CBV @14:31:28.58
CBV.dial SSV @14:31:29.35 Allowing 130s for 25.0ml of remaining 425.0ml @14:31:29.36 Intake.select :open @14:31:31.51 Intake @16.1psia, vacuum from 10.0 to 13.0psi @14:31:31.53 SS.reconfigure :inhaling 0.38ml/s @14:31:31.57 SS.seek 25ml @14:32:42.55 Stroke completed -- waiting for pressure to equalize ... @14:32:42.56 SS.reconfigure :exhale @14:32:42.60 SS.hold @14:33:11.03 Intake.select :close @14:33:11.16 SSV.dial :exhaust @14:33:11.93 Exhaust.select :open @14:33:12.03 SS.seek 0ml @14:33:20.89 Exhaust.select :close @14:33:21.00 SSV.dial CBV @14:33:21.04
CBV.dial SSV @14:33:21.77 Allowing 130s for 25.0ml of remaining 400.0ml @14:33:21.78 Intake.select :open @14:33:23.93 Intake @16.2psia, vacuum from 10.0 to 13.0psi @14:33:23.95 SS.reconfigure :inhaling 0.38ml/s @14:33:23.99 SS.seek 25ml @14:34:29.49 Vacuum too high -- waiting up to 40 seconds @14:34:29.50 SS.reconfigure :exhale @14:34:29.54 SS.hold @14:34:45.79 Intake @16.7psia, vacuum from 10.0 to 13.0psi @14:34:45.80 SS.reconfigure :inhaling 0.34ml/s @14:34:45.84 SS.seek 25ml @14:34:52.00 Stroke completed -- waiting for pressure to equalize ... @14:34:52.01 SS.reconfigure :exhale @14:34:52.05 SS.hold @14:35:08.31 Intake.select :close @14:35:08.45 SSV.dial :exhaust @14:35:09.19 Exhaust.select :open @14:35:09.29 SS.seek 0ml @14:35:17.98 Exhaust.select :close @14:35:18.09 SSV.dial CBV @14:35:18.11
CBV.dial SSV @14:35:18.86 Allowing 130s for 25.0ml of remaining 375.0ml @14:35:18.87 Intake.select :open @14:35:21.02 Intake @16.2psia, vacuum from 10.0 to 13.0psi @14:35:21.04 SS.reconfigure :inhaling 0.34ml/s @14:35:21.08 SS.seek 25ml @14:36:42.50 Stroke completed -- waiting for pressure to equalize ... @14:36:42.51 SS.reconfigure :exhale @14:36:42.55 SS.hold @14:37:08.97 Intake.select :close @14:37:09.10 SSV.dial :exhaust @14:37:09.85 Exhaust.select :open @14:37:09.95 SS.seek 0ml @14:37:18.78 Exhaust.select :close @14:37:18.88 SSV.dial CBV @14:37:18.90
CBV.dial SSV @14:37:19.66 Allowing 130s for 25.0ml of remaining 350.0ml @14:37:19.67 Intake.select :open @14:37:21.82 Intake @16.2psia, vacuum from 10.0 to 13.0psi @14:37:21.83 SS.reconfigure :inhaling 0.34ml/s @14:37:21.87 SS.seek 25ml @14:38:44.68 Stroke completed -- waiting for pressure to equalize ... @14:38:44.69 SS.reconfigure :exhale @14:38:44.73 SS.hold @14:39:13.20 Intake.select :close @14:39:13.33 SSV.dial :exhaust @14:39:14.07 Exhaust.select :open @14:39:14.18 SS.seek 0ml @14:39:23.03 Exhaust.select :close @14:39:23.61 SSV.dial CBV @14:39:23.63
CBV.dial SSV @14:39:24.39 Allowing 130s for 25.0ml of remaining 325.0ml @14:39:24.40 Intake.select :open @14:39:26.55 Intake @16.4psia, vacuum from 10.0 to 13.0psi @14:39:26.57 SS.reconfigure :inhaling 0.34ml/s @14:39:26.61 SS.seek 25ml @14:40:42.77 Vacuum too high -- waiting up to 40 seconds @14:40:42.78 SS.reconfigure :exhale @14:40:42.82 SS.hold @14:40:57.07 SS.reconfigure :inhaling 0.31ml/s @14:40:57.11 SS.seek 25ml @14:41:04.68 Stroke completed -- waiting for pressure to equalize ... @14:41:04.69 SS.reconfigure :exhale @14:41:04.73 SS.hold @14:41:23.10 Intake.select :close @14:41:23.29 SSV.dial :exhaust @14:41:24.04 Exhaust.select :open @14:41:24.15 SS.seek 0ml @14:41:33.15 Exhaust.select :close @14:41:33.25 SSV.dial CBV @14:41:33.27
CBV.dial SSV @14:41:34.03 Allowing 130s for 25.0ml of remaining 300.0ml @14:41:34.04 Intake.select :open @14:41:36.19 Intake @16.3psia, vacuum from 10.0 to 13.0psi @14:41:36.20 SS.reconfigure :inhaling 0.31ml/s @14:41:36.24 SS.seek 25ml @14:43:08.11 Stroke completed -- waiting for pressure to equalize ... @14:43:08.12 SS.reconfigure :exhale @14:43:08.16 SS.hold @14:43:34.52 Intake.select :close @14:43:34.66 SSV.dial :exhaust @14:43:35.40 Exhaust.select :open @14:43:35.51 SS.seek 0ml @14:43:44.33 Exhaust.select :close @14:43:44.44 SSV.dial CBV @14:43:44.48
CBV.dial SSV @14:43:45.20 Allowing 130s for 25.0ml of remaining 275.0ml Intake.select :open @14:43:47.35 Intake @16.3psia, vacuum from 10.0 to 13.0psi @14:43:47.37 SS.reconfigure :inhaling 0.31ml/s @14:43:47.41 SS.seek 25ml @14:45:19.67 Stroke completed -- waiting for pressure to equalize ... @14:45:19.68 SS.reconfigure :exhale @14:45:19.72 SS.hold @14:45:48.75 Intake.select :close @14:45:48.88 SSV.dial :exhaust @14:45:49.63 Exhaust.select :open @14:45:49.74 SS.seek 0ml @14:45:58.58 Exhaust.select :close @14:45:58.68 SSV.dial CBV @14:45:58.70
CBV.dial SSV @14:45:59.48 Allowing 130s for 25.0ml of remaining 250.0ml @14:45:59.49 Intake.select :open @14:46:01.64 Intake @16.3psia, vacuum from 10.0 to 13.0psi @14:46:01.66 SS.reconfigure :inhaling 0.31ml/s @14:46:01.70 SS.seek 25ml @14:47:35.14 Stroke completed -- waiting for pressure to equalize ... @14:47:35.15 SS.reconfigure :exhale @14:47:35.19 SS.hold @14:48:05.67 Intake.select :close @14:48:05.80 SSV.dial :exhaust @14:48:06.57 Exhaust.select :open @14:48:06.67 SS.seek 0ml @14:48:15.45 Exhaust.select :close @14:48:15.55 SSV.dial CBV @14:48:15.58
CBV.dial SSV @14:48:16.35 Allowing 130s for 25.0ml of remaining 225.0ml @14:48:16.36 Intake.select :open @14:48:18.51 Intake @16.3psia, vacuum from 10.0 to 13.0psi @14:48:18.52 SS.reconfigure :inhaling 0.31ml/s @14:48:18.57 SS.seek 25ml @14:49:51.89 Stroke completed -- waiting for pressure to equalize ... @14:49:51.90 SS.reconfigure :exhale @14:49:51.94 SS.hold @14:50:24.43 Intake.select :close @14:50:24.56 SSV.dial :exhaust @14:50:25.32 Exhaust.select :open @14:50:25.42 SS.seek 0ml @14:50:34.48 Exhaust.select :close @14:50:34.58 SSV.dial CBV @14:50:34.61
CBV.dial SSV @14:50:35.37 Allowing 130s for 25.0ml of remaining 200.0ml @14:50:35.38 Intake.select :open @14:50:37.54 Intake @16.4psia, vacuum from 10.0 to 13.0psi @14:50:37.55 SS.reconfigure :inhaling 0.31ml/s @14:50:37.60 SS.seek 25ml @14:52:10.90 Stroke completed -- waiting for pressure to equalize ... @14:52:10.91 SS.reconfigure :exhale @14:52:10.96 SS.hold @14:52:42.00 Intake.select :close @14:52:42.15 SSV.dial :exhaust @14:52:42.90 Exhaust.select :open @14:52:43.01 SS.seek 0ml @14:52:51.82 Exhaust.select :close @14:52:51.92 SSV.dial CBV @14:52:51.95
CBV.dial SSV @14:52:52.72 Allowing 130s for 25.0ml of remaining 175.0ml @14:52:52.73 Intake.select :open @14:52:54.88 Intake @16.3psia, vacuum from 10.0 to 13.0psi @14:52:54.90 SS.reconfigure :inhaling 0.31ml/s @14:52:54.94 SS.seek 25ml @14:54:18.81 Vacuum too high -- waiting up to 40 seconds @14:54:18.82 SS.reconfigure :exhale @14:54:18.86 SS.hold @14:54:37.16 Intake @16.9psia, vacuum from 10.0 to 13.0psi @14:54:37.17 SS.reconfigure :inhaling 0.28ml/s @14:54:37.22 SS.seek 25ml @14:54:46.87 Stroke completed -- waiting for pressure to equalize ... @14:54:46.88 SS.reconfigure :exhale @14:54:46.92 SS.hold @14:55:02.74 SS.reconfigure :default @14:55:02.77 SS.hold @14:55:02.89 Intake.select :close @14:55:03.02 ShallowSampler::Clogged in MAIN -- Filtering too Slowly after 850.0ml @14:55:03.03 SSV.dial :exhaust @14:55:03.77 Exhaust.select :open @14:55:03.88 SS.reconfigure :exhale @14:55:03.92 SS.seek 0ml @14:55:12.65 Exhaust.select :close @14:55:12.75 Sampled 850.0ml @14:55:12.76 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @14:55:12.77 Intake.select :close @14:55:12.84 CSV.dial :waste @14:55:12.91 CS.seek 0ml @14:55:12.99 CSV.dial :air @14:55:13.02 SSV.dial CBV,avoiding: :exhaust @14:55:13.10
CBV.dial SSV @14:55:13.78 CS.seek 10ml @14:55:14.93
CTV.dial :air @14:55:15.73 delay 15 seconds @14:55:30.75 SSV.dial :spit,avoiding: :exhaust @14:55:32.08 CTV.dial CSV,avoiding: :intake @14:55:32.11
SS.seek 0ml @14:55:32.22 SSV.dial CBV,avoiding: :exhaust @14:55:38.28 CBV.dial between SSV and waste2 @14:55:38.66 CSV.dial between air and CTV @14:55:39.45 CS.seek 8.427ml @14:55:43.89 CTV.dial CSV,avoiding: :air @14:55:43.91
CSV.dial CTV @14:55:44.20 CS.reconfigure :pressurize @14:55:44.24 CS.seek 0ml @14:55:58.57 BubblePoint: Intake @39.6psia, Outlet @15.6psia @14:55:58.58 CBV.dial SSV @14:55:58.90 SS.reconfigure :default @14:55:58.95 SS.seek 10ml @14:56:04.54 delay 15 seconds @14:56:19.65 Evacuated: Intake @18.1psia, Outlet @9.7psia @14:56:19.66 SSV.dial :spit,avoiding: :exhaust @14:56:20.98 SS.reconfigure :exhale @14:56:21.02 SS.seek 0ml @14:56:26.33 SS.reconfigure :fast @14:56:26.37 SS.seek 5ml @14:56:29.22 SSV.dial CBV,avoiding: :exhaust @14:56:29.23
CBV.dial SSV @14:56:30.56 CTV.dial :air,avoiding: :intake @14:56:31.92 CSV.dial CTV @14:56:31.95
SS.seek 10ml @14:56:32.94 CSV.dial :air @14:56:33.39 CS.reconfigure :fast @14:56:33.43 CS.seek 0ml @14:56:34.40
SSV.dial :spit,avoiding: :exhaust @14:56:35.72 SS.reconfigure :exhale @14:56:35.76 SS.seek 0ml @14:56:40.72 SS.reconfigure :fast @14:56:40.76 SS.seek 5ml @14:56:41.22 CS.stop @14:56:43.53 SSV.dial CBV,avoiding: :exhaust @14:56:43.55
CBV.dial SSV @14:56:45.88 SSV.dial :spit,avoiding: :exhaust @14:56:47.18 SS.reconfigure :default @14:56:47.23 SS.seek 0ml @14:56:50.44 SS.stop @14:56:50.48 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @14:56:50.49 Intake.select :close @14:56:50.56 CSV.dial :waste @14:56:50.67 SSV.dial CBV,avoiding: :exhaust @14:56:50.68
CBV.dial SSV @14:56:51.33 CS.seek 0ml @14:56:51.39 CSV.dial :air @14:56:52.02
CTV.dial :air @14:56:52.04 delay 15 seconds @14:56:52.12 CS.seek 10ml @14:57:07.05
SSV.dial :spit,avoiding: :exhaust @14:57:08.37 CTV.dial CSV,avoiding: :intake @14:57:08.40
SS.reconfigure :exhale @14:57:08.49 SS.seek 0ml @14:57:08.91 SSV.dial CBV,avoiding: :exhaust @14:57:16.47 CBV.dial between SSV and waste2 @14:57:16.84 CSV.dial between air and CTV @14:57:17.14 CS.seek 8.496ml @14:57:21.36 CTV.dial CSV,avoiding: :air @14:57:21.38
CSV.dial CTV @14:57:21.68 CS.reconfigure :pressurize @14:57:21.73 CS.seek 0ml @14:57:39.72 BubblePoint: Intake @38.6psia, Outlet @14.4psia @14:57:39.73 CBV.dial SSV @14:57:40.06 SS.reconfigure :default @14:57:40.10 SS.seek 10ml @14:57:45.65 delay 15 seconds @14:58:00.76 Evacuated: Intake @18.3psia, Outlet @11.9psia @14:58:00.77 SSV.dial :spit,avoiding: :exhaust @14:58:02.11 SS.reconfigure :exhale @14:58:02.15 SS.seek 0ml @14:58:07.50 SS.reconfigure :fast @14:58:07.54 SS.seek 5ml @14:58:10.37 SSV.dial CBV,avoiding: :exhaust @14:58:10.39
CBV.dial SSV @14:58:11.71 CTV.dial :air,avoiding: :intake @14:58:13.08 CSV.dial CTV @14:58:13.11
SS.seek 10ml @14:58:14.10 CSV.dial :air @14:58:14.56 CS.reconfigure :fast @14:58:14.60 CS.seek 0ml @14:58:15.55
SSV.dial :spit,avoiding: :exhaust @14:58:16.87 SS.reconfigure :exhale @14:58:16.91 SS.seek 0ml @14:58:19.75 CS.stop @14:58:21.89
SS.reconfigure :fast @14:58:21.93 SS.seek 5ml @14:58:24.92 SSV.dial CBV,avoiding: :exhaust @14:58:24.94
CBV.dial SSV @14:58:27.26 SSV.dial :spit,avoiding: :exhaust @14:58:28.57 SS.reconfigure :default @14:58:28.61 SS.seek 0ml @14:58:32.17 SS.stop @14:58:32.21 Lysing: Adding 2.0ml AqMeOH @14:58:32.22 CBV.dial between CSV and waste1,avoiding: :DBV @14:58:33.71 CTV.dial CSV @14:58:35.04 CSV.dial CSR @14:58:35.82 Pulling 1ml of CSR.AqMeOH @14:58:35.83 CSR.select :AqMeOH @14:58:35.94 CS.reconfigure :default @14:58:35.98 CS.seek 1ml @14:58:39.57 delay 5 seconds @14:58:44.58 CSR.select :air @14:58:44.73 CS.seek 6ml @14:58:58.32 delay 2 seconds @14:59:00.33 CSV.dial :waste2 @14:59:00.76 CS.reconfigure :fast @14:59:00.80 CS.seek 0ml @14:59:15.16 CSV.dial CSR @14:59:15.65 Pulling 2ml of CSR.AqMeOH @14:59:15.66 CSR.select :AqMeOH @14:59:15.77 CS.reconfigure :default @14:59:15.81 CS.seek 2ml @14:59:21.72 delay 5 seconds @14:59:26.74 CSR.select :air @14:59:26.89 CS.seek 4.999ml @14:59:35.38 delay 2 seconds @14:59:37.39 CSV.dial CTV @14:59:37.82 CS.seek 1ml @14:59:49.14 Before lysing Collection Puck Heater at 17.5C/39.4psia @14:59:49.15 Heating Puck.from 4, :sh1 in Collection Clamp to 70C for 10:00 @14:59:49.17 CH.seek 70C,8:00 @15:01:45.37 CH.hold 10:00,70C @15:01:45.54 Begun lysing Collection Puck Heater at 68.1C/47.7psia @15:11:45.56 Ended lysing Collection Puck Heater at 70C/46.3psia @15:11:45.58 CS.seek 0ml @15:11:48.97 CTV.dial between spare and air,avoiding: :intake @15:11:50.01 CSV.dial :air @15:11:50.45 CS.seek 5ml @15:12:04.24 CSV.dial CTV @15:12:04.68 delay 2 seconds @15:12:06.69 CSV.dial :air @15:12:07.15 delay 2 seconds @15:12:09.16 CSV.dial CTV @15:12:09.62 delay 2 seconds @15:12:11.63 CSV.dial :air @15:12:12.07 delay 2 seconds @15:12:14.09 CS.seek 0ml @15:12:27.98 Puck @74.4psia after final lysis pressurization @15:12:27.99 Recovering Lysate into Collection syringe @15:12:28.00 CSV.dial CBV @15:12:28.99 CBV.dial CSV @15:12:29.85 CS.seek 5ml @15:12:43.61 delay 5 seconds @15:12:48.63 CTV.dial :air @15:12:49.16 CS.seek 7ml @15:12:55.33 delay 5 seconds @15:13:00.35 CSV.dial :air2 @15:13:00.79 CS.seek 10ml @15:13:09.27 CSV.dial between air and CTV,avoiding: CBV @15:13:10.42 Lysing Complete @15:13:10.48 Storing sh1 puck in Collection Clamp to tube 3 @15:13:10.64 SC.reconfigure :tight @15:13:10.68 SC.seek 3 @15:13:14.27 SC.configure :limp @15:13:14.47 Elbow.reconfigure :tight @15:13:14.51 Elbow.seek 3 @15:13:16.26 Forearm.reconfigure :tight @15:13:16.30 Forearm.seek 3 + 450 counts @15:13:17.27 SE.reconfigure :pick @15:13:17.32 SE.seek :beamBreak @15:13:20.38 SE.configure :default @15:13:20.52 Elbow.reconfigure :limp @15:13:20.56 Forearm.reconfigure :loose @15:13:20.60 Forearm.seek 3 @15:13:20.88 Elbow.hold @15:13:20.94 Elbow.reconfigure :tight @15:13:21.03 SE.seek 1.18 Pucks below Top Plate @15:13:21.47 Forearm.force -20 @15:13:21.50 Forearm.seek 3 + 281 counts @15:13:21.78 Forearm.reconfigure :tight @15:13:21.82 Forearm.seek 3 + 450 counts @15:13:22.09 SE.seek 2.18 Pucks below Top Plate @15:13:22.57 Forearm.reconfigure :loose @15:13:22.62 Forearm.seek :retract @15:13:23.56 Elbow.seek CC @15:13:25.23 Elbow.reconfigure :loose @15:13:25.32 Forearm.seek CC @15:13:26.27 Hand.close @15:13:26.87 CC.seek :open @15:13:31.60 Forearm.seek :retract @15:13:32.57 Elbow.reconfigure :tight @15:13:32.61 Elbow.seek 3 @15:13:34.46 Elbow.reconfigure :limp @15:13:34.50 Forearm.seek 3 @15:13:35.51 Elbow.hold @15:13:35.57 Elbow.reconfigure :tight @15:13:35.61 Hand.open @15:13:36.35 Forearm.force -20 @15:13:36.38 Forearm.seek 3 + 319 counts @15:13:36.67 Forearm.reconfigure :tight @15:13:36.71 Forearm.seek 3 + 450 counts @15:13:36.96 SE.seek :down @15:13:38.09
Forearm.reconfigure :loose @15:13:38.14 Forearm.seek :retract @15:13:39.51 SE.stop @15:13:39.55
Tranferring DA @15:13:39.56 Beginning samplePassC @15:13:39.58 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @15:13:39.75 Elbow.seek FlushPuck::Garage @15:13:41.58 Elbow.reconfigure :loose @15:13:41.66 Forearm.seek :garage @15:13:42.66 Hand.close @15:13:43.35 Forearm.seek :retract @15:13:44.29 Elbow.reconfigure :tight @15:13:44.33 Elbow.seek CC @15:13:47.30 Elbow.reconfigure :loose @15:13:47.39 Forearm.reconfigure :tight @15:13:47.44 Forearm.seek CC @15:13:49.18 CC.seek :closed @15:13:53.76 Collection Clamp onSpring/offSpring for 14/27 samples, on/off torque 2.31 @15:13:53.83 Hand.open @15:13:54.55 Forearm.reconfigure :loose @15:13:54.60 Forearm.seek :retract @15:13:55.57 PSV.dial PSR @15:13:56.94 Pulling 1.5ml of PSR.flush @15:13:56.95 PSR.select :flush @15:13:57.06 PS.seek 1.5ml @15:14:13.12 delay 5 seconds @15:14:18.15 PSV.dial :waste1 @15:14:18.59 PS.seek 0ml @15:14:34.77 PSV.dial PSR @15:14:35.23 PSR.select :air @15:14:35.34 PS.seek 2.5ml @15:15:01.75 PRV.dial CBV,avoiding: :ab2 @15:15:01.79
CBV.dial PRV @15:15:03.44 PSV.dial :mixloop @15:15:04.46 CTV.dial :air,avoiding: :intake @15:15:04.47 PTV.dial :PRVmixing @15:15:05.79 PS.seek 0ml @15:15:32.33 PSV.dial :air @15:15:32.79 PS.seek 2.5ml @15:15:59.08 PSV.dial :mixloop @15:15:59.54 PS.seek 0ml @15:16:25.96 PSV.dial :air @15:16:26.42 PS.seek 2.5ml @15:16:52.71 PSV.dial :mixloop @15:16:53.17 PS.seek 0ml @15:17:19.55 Garaging FlushPuck in Collection Clamp @15:17:19.62 Elbow.reconfigure :tight @15:17:19.67 Elbow.seek CC @15:17:20.01 Elbow.reconfigure :loose @15:17:20.10 Forearm.seek CC @15:17:21.07 Hand.close @15:17:21.62 CC.seek :open @15:17:26.22 Forearm.seek :retract @15:17:27.15 Elbow.reconfigure :tight @15:17:27.19 Elbow.seek :garage @15:17:30.10 Elbow.reconfigure :loose @15:17:30.19 Forearm.reconfigure :tight @15:17:30.24 Forearm.seek :garage @15:17:31.16 Hand.open @15:17:31.80 Forearm.reconfigure :loose @15:17:31.84 Forearm.seek :retract @15:17:32.80 PSV.dial :mixloop @15:17:32.81 PTV.dial :PRVmixing @15:17:32.83 PRV.dial CBV @15:17:32.85
CBV.dial PRV @15:17:32.86 CTV.dial :air,avoiding: :intake @15:17:32.88 Loading lyfil puck from tube 4 into Collection Clamp @15:17:33.10 SC.reconfigure :tight @15:17:33.14 SC.seek 4 @15:17:36.72 SC.configure :limp @15:17:36.91 Elbow.reconfigure :tight @15:17:36.95 Elbow.seek 4 @15:17:38.90 Forearm.reconfigure :tight @15:17:38.94 Forearm.seek 4 + 450 counts @15:17:39.95 SE.reconfigure :pick @15:17:40.00 SE.seek :beamBreak @15:17:41.25 SE.configure :default @15:17:41.39 Elbow.seek 4 @15:17:41.55 Forearm.seek 4 + 300 counts @15:17:41.77 Forearm.reconfigure :loose @15:17:41.81 Hand.close @15:17:42.38 SE.seek :down @15:17:42.57
Forearm.seek :retract @15:17:43.18 SE.stop @15:17:43.72
Elbow.seek CC @15:17:45.36 Elbow.reconfigure :loose @15:17:45.45 Forearm.reconfigure :tight @15:17:45.50 Forearm.seek CC @15:17:46.67 CC.seek :closed @15:17:51.19 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.11 @15:17:51.20 Hand.open @15:17:51.93 Forearm.reconfigure :loose @15:17:51.97 Forearm.seek :retract @15:17:52.93 CTV.dial CSV,avoiding: :intake @15:17:54.28 CSV.dial CTV @15:17:54.59 PS.seek 2.5ml @15:18:20.92 CS.seek 4.5ml @15:18:35.83 delay 8 seconds @15:18:43.85 PSV.dial :air @15:18:44.32 delay 2 seconds @15:18:46.33 PSV.dial :waste2 @15:18:47.06 PS.seek 0ml @15:19:13.39 CBV.dial between PRV and waste1 @15:19:13.73 CSV.dial :air @15:19:14.18 CS.seek 10ml @15:19:28.98 CSV.dial CTV @15:19:29.43 CS.seek 0ml @15:19:55.79 CBV.dial :waste1 @15:19:56.09 delay 5 seconds @15:20:01.11 CTV.dial :air,avoiding: :intake @15:20:02.51 Storing lyfil puck in Collection Clamp to tube 3 @15:20:02.66 SC.reconfigure :tight @15:20:02.71 SC.seek 3 @15:20:06.31 SC.configure :limp @15:20:06.50 Elbow.reconfigure :tight @15:20:06.54 Elbow.seek 3 @15:20:08.32 Forearm.reconfigure :tight @15:20:08.36 Forearm.seek 3 + 450 counts @15:20:09.47 SE.reconfigure :pick @15:20:09.52 SE.seek :beamBreak @15:20:12.45 SE.configure :default @15:20:12.59 Elbow.reconfigure :limp @15:20:12.63 Forearm.reconfigure :loose @15:20:12.67 Forearm.seek 3 @15:20:12.94 Elbow.hold @15:20:13.00 Elbow.reconfigure :tight @15:20:13.09 SE.seek 2.17 Pucks below Top Plate @15:20:13.51 Forearm.force -20 @15:20:13.54 Forearm.seek 3 + 315 counts @15:20:13.82 Forearm.reconfigure :tight @15:20:13.87 Forearm.seek 3 + 450 counts @15:20:14.09 SE.seek 3.17 Pucks below Top Plate @15:20:14.58 Forearm.reconfigure :loose @15:20:14.62 Forearm.seek :retract @15:20:15.57 Elbow.seek CC @15:20:17.26 Elbow.reconfigure :loose @15:20:17.35 Forearm.seek CC @15:20:18.33 Hand.close @15:20:18.99 CC.seek :open @15:20:23.69 Forearm.seek :retract @15:20:24.68 Elbow.reconfigure :tight @15:20:24.72 Elbow.seek 3 @15:20:26.47 Elbow.reconfigure :limp @15:20:26.51 Forearm.seek 3 @15:20:27.48 Elbow.hold @15:20:28.02 Elbow.reconfigure :tight @15:20:28.07 Hand.open @15:20:28.86 Forearm.force -20 @15:20:28.89 Forearm.seek 3 + 319 counts @15:20:29.34 Forearm.reconfigure :tight @15:20:29.38 Forearm.seek 3 + 450 counts @15:20:29.63 SE.seek :down @15:20:30.75
Forearm.reconfigure :loose @15:20:30.79 Forearm.seek :retract @15:20:32.12 SE.stop @15:20:32.15
PSV.dial :air @15:20:32.90 PS.seek 2.5ml @15:20:59.27 delay 1 second @15:21:00.28 PSV.dial :mixloop @15:21:00.72 delay 1 second @15:21:01.73 PSV.dial :air @15:21:02.33 delay 1 second @15:21:03.34 PSV.dial :mixloop @15:21:03.78 delay 1 second @15:21:04.80 PSV.dial :air @15:21:05.25 delay 1 second @15:21:06.27 PS.seek 0ml @15:21:32.71 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @15:21:32.87 Elbow.seek FlushPuck::Garage @15:21:34.70 Elbow.reconfigure :loose @15:21:34.78 Forearm.seek :garage @15:21:35.78 Hand.close @15:21:36.47 Forearm.seek :retract @15:21:37.43 Elbow.reconfigure :tight @15:21:37.47 Elbow.seek PC @15:21:38.73 Elbow.reconfigure :loose @15:21:38.82 Forearm.reconfigure :tight @15:21:38.86 Forearm.seek PC @15:21:40.08 PC.seek :closed @15:21:44.70 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.57 @15:21:44.77 Hand.open @15:21:45.42 Forearm.reconfigure :loose @15:21:45.46 Forearm.seek :retract @15:21:46.47 PRV.dial :air @15:21:46.91 PSV.dial :mixloop @15:21:47.42 PS.seek 0.3ml @15:21:51.26 PSV.dial PSR @15:21:52.35 Pulling 0.4ml of PSR.air @15:21:52.36 PSR.select :air @15:21:52.38 PS.seek 0.7ml @15:21:57.33 delay 3 seconds @15:22:00.34 PSR.select :air @15:22:00.37 PS.seek 2.5ml @15:22:19.61 delay 3 seconds @15:22:22.62 PSV.dial :mixloop @15:22:23.78 PTV.dial :mixing @15:22:24.53 PBV.dial :waste2 @15:22:25.47 PS.seek 0ml @15:22:51.86 Garaging FlushPuck in Processing Clamp @15:22:51.93 Elbow.reconfigure :tight @15:22:51.97 Elbow.seek PC @15:22:52.08 Elbow.reconfigure :loose @15:22:52.17 Forearm.seek PC @15:22:53.15 Hand.close @15:22:53.74 PC.seek :open @15:22:58.43 Forearm.seek :retract @15:22:59.38 Elbow.reconfigure :tight @15:22:59.42 Elbow.seek :garage @15:23:00.77 Elbow.reconfigure :loose @15:23:00.86 Forearm.reconfigure :tight @15:23:00.91 Forearm.seek :garage @15:23:01.90 Hand.open @15:23:02.54 Forearm.reconfigure :loose @15:23:02.58 Forearm.seek :retract @15:23:04.09 Loading da puck from tube 4 into Processing Clamp @15:23:04.30 SC.reconfigure :tight @15:23:04.34 SC.seek 4 @15:23:07.96 SC.configure :limp @15:23:08.15 Elbow.reconfigure :tight @15:23:08.19 Elbow.seek 4 @15:23:10.06 Forearm.reconfigure :tight @15:23:10.11 Forearm.seek 4 + 450 counts @15:23:11.09 SE.reconfigure :pick @15:23:11.14 SE.seek :beamBreak @15:23:12.34 SE.configure :default @15:23:12.49 Elbow.seek 4 @15:23:12.65 Forearm.seek 4 + 300 counts @15:23:12.87 Forearm.reconfigure :loose @15:23:12.91 Hand.close @15:23:13.50 SE.seek :down @15:23:13.69
Forearm.seek :retract @15:23:14.35 SE.stop @15:23:14.66
Elbow.seek PC @15:23:17.21 Elbow.reconfigure :loose @15:23:17.30 Forearm.reconfigure :tight @15:23:17.34 Forearm.seek PC @15:23:18.61 PC.seek :closed @15:23:23.23 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.58 @15:23:23.24 Hand.open @15:23:23.92 Forearm.reconfigure :loose @15:23:23.96 Forearm.seek :retract @15:23:24.95 Heating Puck.from 4, :da in Processing Clamp to 28C for 2:00:00 @15:23:24.96 PH.seek 28C,5:00 @15:23:59.55 PH.hold 2:00:00,28C @15:23:59.61 delay 1:30 @15:25:29.64 PSR.select :air @15:25:29.65 PSV.dial PSR @15:25:30.74 Pulling 0.5ml of PSR.wash PSR.select :wash @15:25:30.86 PS.seek 0.5ml @15:25:36.86 delay 5 seconds @15:25:41.87 PSR.select :air @15:25:41.99 PS.seek 2ml @15:25:58.07 PTV.dial :puck @15:25:58.81 PSV.dial PTV @15:25:59.56 PBV.dial :waste1 @15:26:00.93 PS.seek 1.5ml @15:26:06.79 delay 3 seconds @15:26:09.81 PBV.dial between PSV and waste1 @15:26:10.17 PS.seek 0ml @15:26:26.32 delay 5:00 @15:31:26.34 recoverPSRreagent08 @15:31:26.35 PTV.dial :puck @15:31:26.36 PBV.dial :waste1 @15:31:26.72 delay 30 seconds @15:31:56.75 PBV.dial between PSV and waste1 @15:31:57.10 PSR.select :air @15:31:57.12 PSV.dial PSR @15:31:57.87 PS.reconfigure :fast @15:31:57.91 PS.seek 2.5ml @15:32:21.41 PSV.dial PTV @15:32:22.14 delay 5 seconds @15:32:27.16 PSV.dial PSR @15:32:27.91 delay 5 seconds @15:32:32.93 PSV.dial PTV @15:32:33.68 delay 5 seconds @15:32:38.69 PSV.dial :air @15:32:39.45 PS.seek 0ml @15:33:02.50 PTV.dial :PRVmixing,via: PRV @15:33:04.32 PSV.dial :mixloop @15:33:04.77 PRV.dial :ab1 @15:33:05.51 PS.reconfigure :slow @15:33:05.55 PS.seek 0.15ml @15:33:09.16 delay 2 seconds @15:33:11.18 CBV.dial PRV @15:33:11.21
PRV.dial CBV @15:33:11.74 PS.seek 0.35ml @15:33:16.28 delay 2 seconds @15:33:18.30 CBV.dial between PRV and PSV @15:33:18.61 PRV.dial :air @15:33:19.07 PS.seek 0.9ml @15:33:30.81 PRV.dial :waste2 @15:33:31.54 PS.seek 0.5ml @15:33:40.16 addPMLReagent08 @15:33:40.17 PSV.dial :air @15:33:40.63 PS.reconfigure :default @15:33:40.68 PS.seek 2ml @15:33:56.91 PSV.dial :PML @15:33:57.36 PTV.dial :mixing @15:33:58.10 PBV.dial :waste1 @15:33:58.52 PS.seek 1.85ml @15:34:00.88 PBV.dial between PSV and waste1 @15:34:01.26 PS.seek 0ml @15:34:21.00 delay 2 seconds @15:34:23.02 Beginning samplePassC @15:34:23.03 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @15:34:23.19 Elbow.reconfigure :tight @15:34:23.23 Elbow.seek FlushPuck::Garage @15:34:24.61 Elbow.reconfigure :loose @15:34:24.70 Forearm.seek :garage @15:34:25.68 Hand.close @15:34:26.34 Forearm.seek :retract @15:34:27.35 Elbow.reconfigure :tight @15:34:27.40 Elbow.seek CC @15:34:30.25 Elbow.reconfigure :loose @15:34:30.34 Forearm.reconfigure :tight @15:34:30.38 Forearm.seek CC @15:34:31.64 CC.seek :closed @15:34:36.15 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.35 @15:34:36.22 Hand.open @15:34:36.91 Forearm.reconfigure :loose @15:34:36.95 Forearm.seek :retract @15:34:37.96 PSV.dial PSR @15:34:39.18 Pulling 1.5ml of PSR.flush @15:34:39.19 PSR.select :flush @15:34:39.31 PS.seek 1.5ml @15:34:55.50 delay 5 seconds @15:35:00.52 PSV.dial :waste1 @15:35:00.99 PS.seek 0ml @15:35:17.28 PSV.dial PSR @15:35:17.73 PSR.select :air @15:35:17.84 PS.seek 2.5ml @15:35:44.13 PRV.dial CBV,avoiding: :ab2 @15:35:44.15
CBV.dial PRV @15:35:45.76 PSV.dial :mixloop @15:35:46.78 CTV.dial :air,avoiding: :intake @15:35:46.79 PTV.dial :PRVmixing @15:35:47.54 PS.seek 0ml @15:36:13.87 PSV.dial :air @15:36:14.33 PS.seek 2.5ml @15:36:40.62 PSV.dial :mixloop @15:36:41.08 PS.seek 0ml @15:37:07.46 PSV.dial :air @15:37:07.93 PS.seek 2.5ml @15:37:34.31 PSV.dial :mixloop @15:37:34.77 PS.seek 0ml @15:38:01.09 Garaging FlushPuck in Collection Clamp @15:38:01.16 Elbow.reconfigure :tight @15:38:01.20 Elbow.seek CC @15:38:01.78 Elbow.reconfigure :loose @15:38:01.87 Forearm.seek CC @15:38:02.83 Hand.close @15:38:03.48 CC.seek :open @15:38:08.11 Forearm.seek :retract @15:38:09.10 Elbow.reconfigure :tight @15:38:09.14 Elbow.seek :garage @15:38:12.09 Elbow.reconfigure :loose @15:38:12.18 Forearm.reconfigure :tight @15:38:12.22 Forearm.seek :garage @15:38:13.16 Hand.open @15:38:13.78 Forearm.reconfigure :loose @15:38:13.82 Forearm.seek :retract @15:38:14.81 delayUntil "15:42:23.01" @15:38:14.83 Whole Cell Archive sampling 1000ml @15:38:14.85 Loading wcr puck from tube 4 into Collection Clamp @15:38:15.30 Elbow.reconfigure :tight @15:38:15.35 Elbow.seek 4 @15:38:17.29 Forearm.reconfigure :tight @15:38:17.34 Forearm.seek 4 + 450 counts @15:38:18.40 SE.reconfigure :pick @15:38:18.46 SE.seek :beamBreak @15:38:19.72 SE.configure :default @15:38:19.87 Elbow.seek 4 @15:38:20.04 Forearm.seek 4 + 300 counts @15:38:20.30 Forearm.reconfigure :loose @15:38:20.34 Hand.close @15:38:20.99 SE.seek :down @15:38:21.17 Forearm.seek :retract @15:38:21.93 SE.stop @15:38:22.12 Elbow.seek CC @15:38:23.69 Elbow.reconfigure :loose @15:38:23.78 Forearm.reconfigure :tight @15:38:23.82 Forearm.seek CC @15:38:25.03 CC.seek :closed @15:38:29.57 Collection Clamp onSpring/offSpring for 14/27 samples, on/off torque 2.20 @15:38:29.58 Hand.open @15:38:30.28 Forearm.reconfigure :loose @15:38:30.32 Forearm.seek :retract @15:38:31.27 collectSample 1000ml w/bubble point of 35psi @15:38:31.28 Priming 7ml @15:38:31.29 Intake.select :close @15:38:31.30 Exhaust.select :close @15:38:31.32 SS.hold @15:38:31.51 Calibrating pressure sensors @15:38:31.52 CTV.dial :air @15:38:31.53 SSV.dial :spit,avoiding: :exhaust @15:38:31.55 SS.reconfigure :fast @15:38:31.59 SS.seek 5ml @15:38:34.20 SSV.dial CBV @15:38:34.22 CBV.dial SSV @15:38:35.58 delay 3.8 seconds @15:38:39.51 SSV.dial :spit @15:38:40.82 SS.seek 0ml @15:38:43.60 SSV.dial CBV @15:38:44.92 CTV.dial :intake @15:38:45.69 SSV.dial CBV @15:38:45.71 CBV.dial SSV @15:38:45.72 Allowing 40s for 7.0ml of remaining 7.0ml @15:38:45.73 Intake.select :open @15:38:48.36 Intake @15.1psia, vacuum from 9.6 to 12.6psi @15:38:48.38 SS.reconfigure :inhaling 2.20ml/s @15:38:48.42 SS.seek 7ml @15:38:54.94 Stroke completed -- waiting for pressure to equalize ... @15:38:54.95 SS.reconfigure :exhale @15:38:54.99 SS.hold @15:39:07.56 Intake.select :close @15:39:07.70 SSV.dial :exhaust @15:39:08.46 Exhaust.select :open @15:39:08.57 SS.seek 0ml @15:39:12.52 Exhaust.select :close @15:39:12.62 Sampling 1000.0ml @15:39:12.63 Intake.select :close @15:39:12.64 Exhaust.select :close @15:39:12.66 SS.reconfigure :default @15:39:12.70 SS.hold @15:39:12.90 CTV.dial :intake @15:39:12.92 SSV.dial CBV @15:39:12.93 CBV.dial SSV @15:39:13.71 Allowing 130s for 25.0ml of remaining 1000.0ml @15:39:13.73 Intake.select :open @15:39:15.88 Intake @16.3psia, vacuum from 10.0 to 13.0psi @15:39:15.90 SS.reconfigure :inhaling 2.20ml/s @15:39:15.94 SS.seek 25ml @15:39:21.73 Vacuum too high -- waiting up to 40 seconds @15:39:21.74 SS.reconfigure :exhale @15:39:21.78 SS.hold @15:39:25.08 Intake @15.7psia, vacuum from 10.0 to 13.0psi @15:39:25.09 SS.reconfigure :inhaling 1.98ml/s @15:39:25.14 SS.seek 25ml @15:39:30.70 Vacuum too high -- waiting up to 40 seconds @15:39:30.71 SS.reconfigure :exhale @15:39:30.75 SS.hold @15:39:35.84 Intake @16.2psia, vacuum from 10.0 to 13.0psi @15:39:35.86 SS.reconfigure :inhaling 1.79ml/s @15:39:35.90 SS.seek 25ml @15:39:41.85 Vacuum too high -- waiting up to 40 seconds @15:39:41.86 SS.reconfigure :exhale @15:39:41.91 SS.hold @15:39:46.27 Intake @16.5psia, vacuum from 10.0 to 13.0psi @15:39:46.29 SS.reconfigure :inhaling 1.61ml/s @15:39:46.33 SS.seek 25ml @15:39:47.85 Stroke completed -- waiting for pressure to equalize ... @15:39:47.86 SS.reconfigure :exhale @15:39:47.90 SS.hold @15:39:50.80 Intake.select :close @15:39:50.94 SSV.dial :exhaust @15:39:51.71 Exhaust.select :open @15:39:51.81 SS.seek 0ml @15:40:00.48 Exhaust.select :close @15:40:01.09 SSV.dial CBV @15:40:01.10 CBV.dial SSV @15:40:01.88 Allowing 130s for 25.0ml of remaining 975.0ml @15:40:01.89 Intake.select :open @15:40:04.05 Intake @16.4psia, vacuum from 10.0 to 13.0psi @15:40:04.07 SS.reconfigure :inhaling 1.61ml/s @15:40:04.11 SS.seek 25ml @15:40:13.54 Vacuum too high -- waiting up to 40 seconds @15:40:13.55 SS.reconfigure :exhale @15:40:13.60 SS.hold @15:40:16.25 Intake @15.5psia, vacuum from 10.0 to 13.0psi @15:40:16.27 SS.reconfigure :inhaling 1.45ml/s @15:40:16.32 SS.seek 25ml @15:40:27.00 Stroke completed -- waiting for pressure to equalize ... @15:40:27.01 SS.reconfigure :exhale @15:40:27.05 SS.hold @15:40:34.52 Intake.select :close @15:40:34.66 SSV.dial :exhaust @15:40:35.42 Exhaust.select :open @15:40:35.54 SS.seek 0ml @15:40:44.27 Exhaust.select :close @15:40:44.37 SSV.dial CBV @15:40:44.40 CBV.dial SSV @15:40:45.16 Allowing 130s for 25.0ml of remaining 950.0ml @15:40:45.17 Intake.select :open @15:40:47.33 Intake @16.4psia, vacuum from 10.0 to 13.0psi @15:40:47.35 SS.reconfigure :inhaling 1.45ml/s @15:40:47.39 SS.seek 25ml @15:40:57.76 Vacuum too high -- waiting up to 40 seconds @15:40:57.77 SS.reconfigure :exhale @15:40:57.81 SS.hold @15:41:00.89 Intake @15.7psia, vacuum from 10.0 to 13.0psi @15:41:00.91 SS.reconfigure :inhaling 1.30ml/s @15:41:00.95 SS.seek 25ml @15:41:12.37 Stroke completed -- waiting for pressure to equalize ... @15:41:12.39 SS.reconfigure :exhale @15:41:12.43 SS.hold @15:41:19.81 Intake.select :close @15:41:19.95 SSV.dial :exhaust @15:41:20.72 Exhaust.select :open @15:41:20.83 SS.seek 0ml @15:41:29.86 Exhaust.select :close @15:41:29.97 SSV.dial CBV @15:41:30.02 CBV.dial SSV @15:41:30.74 Allowing 130s for 25.0ml of remaining 925.0ml @15:41:30.75 Intake.select :open @15:41:32.90 Intake @16.3psia, vacuum from 10.0 to 13.0psi @15:41:32.92 SS.reconfigure :inhaling 1.30ml/s @15:41:32.96 SS.seek 25ml @15:41:46.66 Vacuum too high -- waiting up to 40 seconds @15:41:46.67 SS.reconfigure :exhale @15:41:46.72 SS.hold @15:41:50.80 Intake @15.7psia, vacuum from 10.0 to 13.0psi @15:41:50.82 SS.reconfigure :inhaling 1.17ml/s @15:41:50.86 SS.seek 25ml @15:41:59.70 Stroke completed -- waiting for pressure to equalize ... @15:41:59.71 SS.reconfigure :exhale @15:41:59.76 SS.hold @15:42:05.32 Intake.select :close @15:42:05.46 SSV.dial :exhaust @15:42:06.22 Exhaust.select :open @15:42:06.33 SS.seek 0ml @15:42:15.37 Exhaust.select :close @15:42:15.48 SSV.dial CBV @15:42:15.50 CBV.dial SSV @15:42:16.28 Allowing 130s for 25.0ml of remaining 900.0ml @15:42:16.29 Intake.select :open @15:42:18.45 Intake @16.3psia, vacuum from 10.0 to 13.0psi @15:42:18.47 SS.reconfigure :inhaling 1.17ml/s @15:42:18.51 SS.seek 25ml @15:42:23.02 recoverPRVreagent08 @15:42:23.03 PTV.dial :mixing @15:42:23.79 PBV.dial :waste1 @15:42:24.15 delay 30 seconds @15:42:36.19 Vacuum too high -- waiting up to 40 seconds @15:42:36.21 SS.reconfigure :exhale @15:42:36.25 SS.hold @15:42:39.83 Intake @15.4psia, vacuum from 9.9 to 12.9psi @15:42:39.85 SS.reconfigure :inhaling 1.06ml/s @15:42:39.89 SS.seek 25ml @15:42:46.64 Stroke completed -- waiting for pressure to equalize ... @15:42:46.65 SS.reconfigure :exhale @15:42:46.69 SS.hold @15:42:52.24 Intake.select :close @15:42:52.38 SSV.dial :exhaust @15:42:53.14 Exhaust.select :open @15:42:53.25 SS.seek 0ml @15:42:54.17 PBV.dial between PSV and waste1 @15:42:54.54 PSV.dial :air @15:42:55.15 PS.reconfigure :fast @15:42:55.20 PS.seek 2.5ml @15:43:02.26 Exhaust.select :close @15:43:02.37 SSV.dial CBV @15:43:02.39 CBV.dial SSV @15:43:03.17 Allowing 130s for 25.0ml of remaining 875.0ml @15:43:03.18 Intake.select :open @15:43:05.34 Intake @16.1psia, vacuum from 10.0 to 13.0psi @15:43:05.36 SS.reconfigure :inhaling 1.06ml/s @15:43:05.40 SS.seek 25ml @15:43:19.02 PSV.dial :mixloop @15:43:19.48 delay 2 seconds @15:43:21.49 PSV.dial :air @15:43:21.96 delay 2 seconds @15:43:23.98 PSV.dial :mixloop @15:43:24.44 delay 2 seconds @15:43:26.45 PSV.dial :air @15:43:26.92 PS.seek 0ml @15:43:31.21 Stroke completed -- waiting for pressure to equalize ... @15:43:31.23 SS.reconfigure :exhale @15:43:31.27 SS.hold @15:43:40.30 Intake.select :close @15:43:40.44 SSV.dial :exhaust @15:43:41.20 Exhaust.select :open @15:43:41.31 SS.seek 0ml @15:43:50.12 Exhaust.select :close @15:43:50.23 SSV.dial CBV @15:43:50.27 CBV.dial SSV @15:43:50.32 washCycleDA #1 of 2 @15:43:50.34 PSR.select :air @15:43:50.36 PSV.dial PSR @15:43:51.05 Allowing 130s for 25.0ml of remaining 850.0ml @15:43:51.06 Intake.select :open @15:43:51.73 Pulling 0.5ml of PSR.wash @15:43:51.74 PSR.select :wash @15:43:51.85 PS.reconfigure :default @15:43:51.90 PS.seek 0.5ml @15:43:53.21 Intake @16.0psia, vacuum from 10.0 to 13.0psi @15:43:53.23 SS.reconfigure :inhaling 1.06ml/s @15:43:53.28 SS.seek 25ml @15:43:57.90 delay 5 seconds @15:44:02.92 PSR.select :air @15:44:03.03 PS.seek 2ml @15:44:19.14 PTV.dial :puck @15:44:19.65 Stroke completed -- waiting for pressure to equalize ... @15:44:19.67 SS.reconfigure :exhale @15:44:19.71 SS.hold @15:44:19.91 PSV.dial PTV @15:44:20.66 PBV.dial :waste1 @15:44:21.08 PS.seek 1.5ml @15:44:27.02 delay 3 seconds @15:44:28.27 Intake.select :close @15:44:28.41 SSV.dial :exhaust @15:44:29.17 Exhaust.select :open @15:44:29.28 SS.seek 0ml @15:44:30.03 PBV.dial between PSV and waste1 @15:44:30.42 PS.seek 0ml @15:44:38.26 Exhaust.select :close @15:44:38.37 SSV.dial CBV @15:44:38.39 CBV.dial SSV @15:44:39.17 Allowing 130s for 25.0ml of remaining 825.0ml @15:44:39.18 Intake.select :open @15:44:41.34 Intake @16.0psia, vacuum from 10.0 to 13.0psi @15:44:41.35 SS.reconfigure :inhaling 1.06ml/s @15:44:41.40 SS.seek 25ml @15:44:46.52 delay 2:00 @15:45:07.28 Stroke completed -- waiting for pressure to equalize ... @15:45:07.29 SS.reconfigure :exhale @15:45:07.33 SS.hold @15:45:17.02 Intake.select :close @15:45:17.16 SSV.dial :exhaust @15:45:17.92 Exhaust.select :open @15:45:18.03 SS.seek 0ml @15:45:27.07 Exhaust.select :close @15:45:27.18 SSV.dial CBV @15:45:27.20 CBV.dial SSV @15:45:27.98 Allowing 130s for 25.0ml of remaining 800.0ml @15:45:27.99 Intake.select :open @15:45:30.15 Intake @15.9psia, vacuum from 10.0 to 13.0psi @15:45:30.17 SS.reconfigure :inhaling 1.06ml/s @15:45:30.21 SS.seek 25ml @15:45:55.87 Stroke completed -- waiting for pressure to equalize ... @15:45:55.88 SS.reconfigure :exhale @15:45:55.92 SS.hold @15:46:05.58 Intake.select :close @15:46:05.72 SSV.dial :exhaust @15:46:06.48 Exhaust.select :open @15:46:06.59 SS.seek 0ml @15:46:15.32 Exhaust.select :close @15:46:15.43 SSV.dial CBV @15:46:15.45 CBV.dial SSV @15:46:16.23 Allowing 130s for 25.0ml of remaining 775.0ml @15:46:16.24 Intake.select :open @15:46:18.40 Intake @16.0psia, vacuum from 10.0 to 13.0psi @15:46:18.42 SS.reconfigure :inhaling 1.06ml/s @15:46:18.47 SS.seek 25ml @15:46:42.38 Vacuum too high -- waiting up to 40 seconds @15:46:42.39 SS.reconfigure :exhale @15:46:42.43 SS.hold @15:46:46.58 recoverPSRreagent08 @15:46:46.59 PTV.dial :puck @15:46:46.60 PBV.dial :waste1 @15:46:46.66 Intake @14.8psia, vacuum from 9.3 to 12.3psi @15:46:46.68 SS.reconfigure :inhaling 0.95ml/s @15:46:46.72 SS.seek 25ml @15:46:46.99 delay 30 seconds @15:46:49.85 Stroke completed -- waiting for pressure to equalize ... @15:46:49.86 SS.reconfigure :exhale @15:46:49.91 SS.hold @15:46:54.68 Intake.select :close @15:46:54.82 SSV.dial :exhaust @15:46:55.57 Exhaust.select :open @15:46:55.68 SS.seek 0ml @15:47:04.59 Exhaust.select :close @15:47:04.69 SSV.dial CBV @15:47:04.71 CBV.dial SSV @15:47:05.48 Allowing 130s for 25.0ml of remaining 750.0ml @15:47:05.49 Intake.select :open @15:47:07.65 Intake @16.0psia, vacuum from 10.0 to 13.0psi @15:47:07.66 SS.reconfigure :inhaling 0.95ml/s @15:47:07.71 SS.seek 25ml @15:47:17.01 PBV.dial between PSV and waste1 @15:47:17.38 PSR.select :air @15:47:17.40 PSV.dial PSR @15:47:18.16 PS.reconfigure :fast @15:47:18.21 PS.seek 2.5ml @15:47:36.59 Stroke completed -- waiting for pressure to equalize ... @15:47:36.60 SS.reconfigure :exhale @15:47:36.64 SS.hold @15:47:42.18 PSV.dial PTV @15:47:42.94 delay 5 seconds @15:47:45.17 Intake.select :close @15:47:45.31 SSV.dial :exhaust @15:47:46.07 Exhaust.select :open @15:47:46.18 SS.seek 0ml @15:47:47.96 PSV.dial PSR @15:47:48.72 delay 5 seconds @15:47:53.74 PSV.dial PTV @15:47:54.49 delay 5 seconds @15:47:55.21 Exhaust.select :close @15:47:55.32 SSV.dial CBV @15:47:55.34 CBV.dial SSV @15:47:56.12 Allowing 130s for 25.0ml of remaining 725.0ml @15:47:56.13 Intake.select :open @15:47:58.28 Intake @16.0psia, vacuum from 10.0 to 13.0psi @15:47:58.81 SS.reconfigure :inhaling 0.95ml/s @15:47:58.85 SS.seek 25ml @15:47:59.50 PSV.dial :air @15:48:00.27 PS.seek 0ml @15:48:23.67 washCycleDA #2 of 2 @15:48:23.68 PSR.select :air @15:48:23.70 PSV.dial PSR @15:48:25.07 Pulling 0.5ml of PSR.wash @15:48:25.08 PSR.select :wash @15:48:25.19 PS.reconfigure :default @15:48:25.23 PS.seek 0.5ml @15:48:27.19 Stroke completed -- waiting for pressure to equalize ... @15:48:27.21 SS.reconfigure :exhale @15:48:27.25 SS.hold @15:48:31.11 delay 5 seconds @15:48:36.13 PSR.select :air @15:48:36.24 PS.seek 2ml @15:48:36.93 Intake.select :close @15:48:37.07 SSV.dial :exhaust @15:48:37.84 Exhaust.select :open @15:48:37.95 SS.seek 0ml @15:48:46.76 Exhaust.select :close @15:48:46.86 SSV.dial CBV @15:48:46.88 CBV.dial SSV @15:48:47.65 Allowing 130s for 25.0ml of remaining 700.0ml @15:48:47.66 Intake.select :open @15:48:49.82 Intake @15.9psia, vacuum from 10.0 to 13.0psi @15:48:49.84 SS.reconfigure :inhaling 0.95ml/s @15:48:49.88 SS.seek 25ml @15:48:52.43 PTV.dial :puck @15:48:52.44 PSV.dial PTV @15:48:53.31 PBV.dial :waste1 @15:48:53.75 PS.seek 1.5ml @15:48:59.77 delay 3 seconds @15:49:02.78 PBV.dial between PSV and waste1 @15:49:03.16 PS.seek 0ml @15:49:18.60 Stroke completed -- waiting for pressure to equalize ... @15:49:18.61 SS.reconfigure :exhale @15:49:18.65 SS.hold @15:49:19.33 delay 2:00 @15:49:28.42 Intake.select :close @15:49:28.56 SSV.dial :exhaust @15:49:29.32 Exhaust.select :open @15:49:29.43 SS.seek 0ml @15:49:38.38 Exhaust.select :close @15:49:38.49 SSV.dial CBV @15:49:38.53 CBV.dial SSV @15:49:39.27 Allowing 130s for 25.0ml of remaining 675.0ml @15:49:39.29 Intake.select :open @15:49:41.44 Intake @16.0psia, vacuum from 10.0 to 13.0psi @15:49:41.46 SS.reconfigure :inhaling 0.95ml/s @15:49:41.50 SS.seek 25ml @15:50:06.72 Vacuum too high -- waiting up to 40 seconds @15:50:06.73 SS.reconfigure :exhale @15:50:06.77 SS.hold @15:50:11.63 Intake @15.3psia, vacuum from 9.8 to 12.8psi @15:50:11.65 SS.reconfigure :inhaling 0.87ml/s @15:50:11.69 SS.seek 25ml @15:50:15.21 Stroke completed -- waiting for pressure to equalize ... @15:50:15.22 SS.reconfigure :exhale @15:50:15.26 SS.hold @15:50:20.63 Intake.select :close @15:50:20.78 SSV.dial :exhaust @15:50:21.54 Exhaust.select :open @15:50:21.65 SS.seek 0ml @15:50:30.46 Exhaust.select :close @15:50:30.56 SSV.dial CBV @15:50:30.58 CBV.dial SSV @15:50:31.35 Allowing 130s for 25.0ml of remaining 650.0ml @15:50:31.36 Intake.select :open @15:50:33.52 Intake @16.0psia, vacuum from 10.0 to 13.0psi @15:50:33.54 SS.reconfigure :inhaling 0.87ml/s @15:50:33.58 SS.seek 25ml @15:51:05.14 Stroke completed -- waiting for pressure to equalize ... @15:51:05.16 SS.reconfigure :exhale @15:51:05.20 SS.hold @15:51:14.88 Intake.select :close @15:51:15.02 SSV.dial :exhaust @15:51:15.78 Exhaust.select :open @15:51:15.89 SS.seek 0ml @15:51:19.35 recoverPSRreagent08 @15:51:19.36 PTV.dial :puck @15:51:19.37 PBV.dial :waste1 @15:51:19.73 delay 30 seconds @15:51:24.74 Exhaust.select :close @15:51:24.84 SSV.dial CBV @15:51:24.87 CBV.dial SSV @15:51:25.63 Allowing 130s for 25.0ml of remaining 625.0ml @15:51:25.64 Intake.select :open @15:51:27.80 Intake @15.9psia, vacuum from 10.0 to 13.0psi @15:51:27.81 SS.reconfigure :inhaling 0.87ml/s @15:51:27.86 SS.seek 25ml @15:51:49.76 PBV.dial between PSV and waste1 @15:51:50.12 PSR.select :air @15:51:50.14 PSV.dial PSR @15:51:50.89 PS.reconfigure :fast @15:51:50.94 PS.seek 2.5ml @15:51:59.44 Stroke completed -- waiting for pressure to equalize ... @15:51:59.45 SS.reconfigure :exhale @15:51:59.49 SS.hold @15:52:09.29 Intake.select :close @15:52:09.43 SSV.dial :exhaust @15:52:10.20 Exhaust.select :open @15:52:10.31 SS.seek 0ml @15:52:14.85 PSV.dial PTV @15:52:15.62 delay 5 seconds @15:52:19.27 Exhaust.select :close @15:52:19.37 SSV.dial CBV @15:52:19.39 CBV.dial SSV @15:52:20.16 Allowing 130s for 25.0ml of remaining 600.0ml @15:52:20.17 Intake.select :open @15:52:20.64 PSV.dial PSR @15:52:21.40 delay 5 seconds @15:52:22.33 Intake @15.9psia, vacuum from 10.0 to 13.0psi @15:52:22.35 SS.reconfigure :inhaling 0.87ml/s @15:52:22.39 SS.seek 25ml @15:52:26.42 PSV.dial PTV @15:52:27.31 delay 5 seconds @15:52:32.33 PSV.dial :air @15:52:33.10 PS.seek 0ml @15:52:53.39 Stroke completed -- waiting for pressure to equalize ... @15:52:53.40 SS.reconfigure :exhale @15:52:53.45 SS.hold @15:52:56.21 getPMLReagent08 @15:52:56.25 PRV.dial :air @15:52:56.70 delay 5 seconds @15:53:01.71 PTV.dial :PRVmixing @15:53:03.04 delay 5 seconds @15:53:03.69 Intake.select :close @15:53:03.83 SSV.dial :exhaust @15:53:04.58 Exhaust.select :open @15:53:04.69 SS.seek 0ml @15:53:08.06 PRV.dial :ab2 @15:53:08.49 PTV.dial :PRVmixing @15:53:08.51 PSV.dial :mixloop @15:53:08.97 PS.reconfigure :default @15:53:09.02 PS.seek 0.3ml @15:53:12.92 delay 2 seconds @15:53:13.48 Exhaust.select :close @15:53:13.60 SSV.dial CBV @15:53:13.61 CBV.dial SSV @15:53:14.38 Allowing 130s for 25.0ml of remaining 575.0ml @15:53:14.39 Intake.select :open @15:53:14.93 PRV.dial :air @15:53:15.44 PS.seek 0.55ml @15:53:16.54 Intake @15.9psia, vacuum from 10.0 to 13.0psi @15:53:16.56 SS.reconfigure :inhaling 0.87ml/s @15:53:16.61 SS.seek 25ml @15:53:18.86 addPMLReagent08 @15:53:18.87 PSV.dial :air @15:53:19.38 PS.seek 2ml @15:53:35.23 PSV.dial :PML @15:53:35.68 PTV.dial :mixing @15:53:36.43 PBV.dial :waste1 @15:53:36.85 PS.seek 1.85ml @15:53:39.20 PBV.dial between PSV and waste1 @15:53:39.58 PS.seek 0ml @15:53:47.53 Vacuum too high -- waiting up to 40 seconds @15:53:47.54 SS.reconfigure :exhale @15:53:47.58 SS.hold @15:53:53.23 Intake @15.0psia, vacuum from 9.5 to 12.5psi @15:53:53.24 SS.reconfigure :inhaling 0.78ml/s @15:53:53.29 SS.seek 25ml @15:53:54.06 Stroke completed -- waiting for pressure to equalize ... @15:53:54.08 SS.reconfigure :exhale @15:53:54.12 SS.hold @15:53:58.89 Intake.select :close @15:53:59.03 SSV.dial :exhaust @15:53:59.29 delay 2 seconds @15:53:59.80 Exhaust.select :open @15:53:59.91 SS.seek 0ml @15:54:01.31 delay 8:00 @15:54:08.72 Exhaust.select :close @15:54:08.83 SSV.dial CBV @15:54:08.88 CBV.dial SSV @15:54:09.60 Allowing 130s for 25.0ml of remaining 550.0ml @15:54:09.61 Intake.select :open @15:54:11.76 Intake @16.0psia, vacuum from 10.0 to 13.0psi @15:54:11.78 SS.reconfigure :inhaling 0.78ml/s @15:54:11.82 SS.seek 25ml @15:54:46.47 Stroke completed -- waiting for pressure to equalize ... @15:54:46.48 SS.reconfigure :exhale @15:54:46.52 SS.hold @15:54:56.52 Intake.select :close @15:54:56.71 SSV.dial :exhaust @15:54:57.48 Exhaust.select :open @15:54:57.59 SS.seek 0ml @15:55:06.45 Exhaust.select :close @15:55:06.56 SSV.dial CBV @15:55:06.58 CBV.dial SSV @15:55:07.36 Allowing 130s for 25.0ml of remaining 525.0ml @15:55:07.37 Intake.select :open @15:55:09.53 Intake @15.9psia, vacuum from 10.0 to 13.0psi @15:55:09.55 SS.reconfigure :inhaling 0.78ml/s @15:55:09.59 SS.seek 25ml @15:55:44.14 Stroke completed -- waiting for pressure to equalize ... @15:55:44.15 SS.reconfigure :exhale @15:55:44.19 SS.hold @15:55:56.21 Intake.select :close @15:55:56.36 SSV.dial :exhaust @15:55:57.11 Exhaust.select :open @15:55:57.22 SS.seek 0ml @15:56:06.03 Exhaust.select :close @15:56:06.13 SSV.dial CBV @15:56:06.18 CBV.dial SSV @15:56:06.92 Allowing 130s for 25.0ml of remaining 500.0ml @15:56:06.93 Intake.select :open @15:56:09.09 Intake @15.9psia, vacuum from 10.0 to 13.0psi @15:56:09.11 SS.reconfigure :inhaling 0.78ml/s @15:56:09.15 SS.seek 25ml @15:56:43.84 Stroke completed -- waiting for pressure to equalize ... @15:56:43.85 SS.reconfigure :exhale @15:56:43.89 SS.hold @15:56:55.04 Intake.select :close @15:56:55.18 SSV.dial :exhaust @15:56:55.93 Exhaust.select :open @15:56:56.04 SS.seek 0ml @15:57:04.87 Exhaust.select :close @15:57:04.98 SSV.dial CBV @15:57:05.00 CBV.dial SSV @15:57:05.78 Allowing 130s for 25.0ml of remaining 475.0ml @15:57:05.79 Intake.select :open @15:57:07.95 Intake @15.9psia, vacuum from 10.0 to 13.0psi @15:57:07.97 SS.reconfigure :inhaling 0.78ml/s @15:57:08.01 SS.seek 25ml @15:57:40.58 Vacuum too high -- waiting up to 40 seconds @15:57:40.59 SS.reconfigure :exhale @15:57:40.64 SS.hold @15:57:46.31 Intake @15.4psia, vacuum from 9.9 to 12.9psi @15:57:46.75 SS.reconfigure :inhaling 0.70ml/s @15:57:46.89 SS.seek 25ml @15:57:49.53 Stroke completed -- waiting for pressure to equalize ... @15:57:49.54 SS.reconfigure :exhale @15:57:49.58 SS.hold @15:57:55.13 Intake.select :close @15:57:55.27 SSV.dial :exhaust @15:57:56.03 Exhaust.select :open @15:57:56.14 SS.seek 0ml @15:58:04.95 Exhaust.select :close @15:58:05.06 SSV.dial CBV @15:58:05.09 CBV.dial SSV @15:58:05.84 Allowing 130s for 25.0ml of remaining 450.0ml @15:58:05.85 Intake.select :open @15:58:08.01 Intake @15.9psia, vacuum from 10.0 to 13.0psi @15:58:08.03 SS.reconfigure :inhaling 0.70ml/s @15:58:08.07 SS.seek 25ml @15:58:45.98 Stroke completed -- waiting for pressure to equalize ... @15:58:45.99 SS.reconfigure :exhale @15:58:46.03 SS.hold @15:58:57.18 Intake.select :close @15:58:57.32 SSV.dial :exhaust @15:58:58.09 Exhaust.select :open @15:58:58.20 SS.seek 0ml @15:59:07.07 Exhaust.select :close @15:59:07.18 SSV.dial CBV @15:59:07.20 CBV.dial SSV @15:59:07.98 Allowing 130s for 25.0ml of remaining 425.0ml @15:59:07.99 Intake.select :open @15:59:10.15 Intake @16.0psia, vacuum from 10.0 to 13.0psi @15:59:10.16 SS.reconfigure :inhaling 0.70ml/s @15:59:10.21 SS.seek 25ml @15:59:48.04 Stroke completed -- waiting for pressure to equalize ... @15:59:48.05 SS.reconfigure :exhale @15:59:48.09 SS.hold @16:00:00.73 Intake.select :close @16:00:00.87 SSV.dial :exhaust @16:00:01.63 Exhaust.select :open @16:00:01.74 SS.seek 0ml @16:00:10.82 Exhaust.select :close @16:00:10.93 SSV.dial CBV @16:00:10.95 CBV.dial SSV @16:00:11.73 Allowing 130s for 25.0ml of remaining 400.0ml @16:00:11.74 Intake.select :open @16:00:13.89 Intake @15.9psia, vacuum from 10.0 to 13.0psi @16:00:13.91 SS.reconfigure :inhaling 0.70ml/s @16:00:13.95 SS.seek 25ml @16:00:51.24 Stroke completed -- waiting for pressure to equalize ... @16:00:51.25 SS.reconfigure :exhale @16:00:51.30 SS.hold @16:01:04.36 Intake.select :close @16:01:04.50 SSV.dial :exhaust @16:01:05.26 Exhaust.select :open @16:01:05.37 SS.seek 0ml @16:01:14.44 Exhaust.select :close @16:01:14.55 SSV.dial CBV @16:01:14.57 CBV.dial SSV @16:01:15.35 Allowing 130s for 25.0ml of remaining 375.0ml @16:01:15.37 Intake.select :open @16:01:17.52 Intake @16.0psia, vacuum from 10.0 to 13.0psi @16:01:17.54 SS.reconfigure :inhaling 0.70ml/s @16:01:17.58 SS.seek 25ml @16:01:49.23 Vacuum too high -- waiting up to 40 seconds @16:01:49.25 SS.reconfigure :exhale @16:01:49.29 SS.hold @16:01:55.78 Intake @15.5psia, vacuum from 10.0 to 13.0psi @16:01:55.80 SS.reconfigure :inhaling 0.63ml/s @16:01:55.84 SS.seek 25ml @16:02:01.33 recoverPRVreagent08 @16:02:01.34 PTV.dial :mixing @16:02:01.35 PBV.dial :waste1 @16:02:01.72 delay 30 seconds @16:02:02.72 Stroke completed -- waiting for pressure to equalize ... @16:02:02.74 SS.reconfigure :exhale @16:02:02.78 SS.hold @16:02:10.15 Intake.select :close @16:02:10.29 SSV.dial :exhaust @16:02:11.05 Exhaust.select :open @16:02:11.16 SS.seek 0ml @16:02:20.05 Exhaust.select :close @16:02:20.16 SSV.dial CBV @16:02:20.18 CBV.dial SSV @16:02:20.96 Allowing 130s for 25.0ml of remaining 350.0ml @16:02:20.97 Intake.select :open @16:02:23.13 Intake @16.1psia, vacuum from 10.0 to 13.0psi @16:02:23.15 SS.reconfigure :inhaling 0.63ml/s @16:02:23.19 SS.seek 25ml @16:02:31.74 PBV.dial between PSV and waste1 @16:02:32.10 PSV.dial :air @16:02:32.57 PS.reconfigure :fast @16:02:32.61 PS.seek 2.5ml @16:02:56.69 PSV.dial :mixloop @16:02:57.14 delay 2 seconds @16:02:59.16 PSV.dial :air @16:02:59.61 delay 2 seconds @16:03:01.63 PSV.dial :mixloop @16:03:02.08 delay 2 seconds @16:03:04.10 PSV.dial :air @16:03:04.57 PS.seek 0ml @16:03:05.28 Stroke completed -- waiting for pressure to equalize ... @16:03:05.30 SS.reconfigure :exhale @16:03:05.34 SS.hold @16:03:18.40 Intake.select :close @16:03:18.55 SSV.dial :exhaust @16:03:19.32 Exhaust.select :open @16:03:19.43 SS.seek 0ml @16:03:28.12 washCycleDA #1 of 3 @16:03:28.13 PSR.select :air @16:03:28.15 PSV.dial PSR @16:03:28.27 Exhaust.select :close @16:03:28.38 SSV.dial CBV @16:03:28.40 CBV.dial SSV @16:03:29.18 Allowing 130s for 25.0ml of remaining 325.0ml @16:03:29.19 Intake.select :open @16:03:29.52 Pulling 0.5ml of PSR.wash @16:03:29.53 PSR.select :wash @16:03:29.65 PS.reconfigure :default @16:03:29.69 PS.seek 0.5ml @16:03:31.35 Intake @16.1psia, vacuum from 10.0 to 13.0psi @16:03:31.37 SS.reconfigure :inhaling 0.63ml/s @16:03:31.41 SS.seek 25ml @16:03:35.63 delay 5 seconds @16:03:40.65 PSR.select :air @16:03:40.76 PS.seek 2ml @16:03:56.84 PTV.dial :puck @16:03:57.59 PSV.dial PTV @16:03:58.34 PBV.dial :waste1 @16:03:58.78 PS.seek 1.5ml @16:04:04.69 delay 3 seconds @16:04:07.70 PBV.dial between PSV and waste1 @16:04:08.08 PS.seek 0ml @16:04:10.64 Vacuum too high -- waiting up to 40 seconds @16:04:10.65 SS.reconfigure :exhale @16:04:10.69 SS.hold @16:04:17.21 Intake @15.6psia, vacuum from 10.0 to 13.0psi @16:04:17.23 SS.reconfigure :inhaling 0.57ml/s @16:04:17.27 SS.seek 25ml @16:04:20.74 Stroke completed -- waiting for pressure to equalize ... @16:04:20.75 SS.reconfigure :exhale @16:04:20.79 SS.hold @16:04:24.40 delay 2:00 @16:04:27.35 Intake.select :close @16:04:27.49 SSV.dial :exhaust @16:04:28.25 Exhaust.select :open @16:04:28.36 SS.seek 0ml @16:04:37.33 Exhaust.select :close @16:04:37.44 SSV.dial CBV @16:04:37.46 CBV.dial SSV @16:04:38.24 Allowing 130s for 25.0ml of remaining 300.0ml @16:04:38.25 Intake.select :open @16:04:40.41 Intake @16.1psia, vacuum from 10.0 to 13.0psi @16:04:40.43 SS.reconfigure :inhaling 0.57ml/s @16:04:40.47 SS.seek 25ml @16:05:26.08 Stroke completed -- waiting for pressure to equalize ... @16:05:26.09 SS.reconfigure :exhale @16:05:26.13 SS.hold @16:05:38.77 Intake.select :close @16:05:38.91 SSV.dial :exhaust @16:05:39.67 Exhaust.select :open @16:05:39.78 SS.seek 0ml @16:05:49.09 Exhaust.select :close @16:05:49.20 SSV.dial CBV @16:05:49.22 CBV.dial SSV @16:05:50.00 Allowing 130s for 25.0ml of remaining 275.0ml @16:05:50.02 Intake.select :open @16:05:52.22 Intake @16.0psia, vacuum from 10.0 to 13.0psi @16:05:52.24 SS.reconfigure :inhaling 0.57ml/s @16:05:52.28 SS.seek 25ml @16:06:24.43 recoverPSRreagent08 @16:06:24.44 PTV.dial :puck @16:06:24.45 PBV.dial :waste1 @16:06:24.83 delay 30 seconds @16:06:38.27 Stroke completed -- waiting for pressure to equalize ... @16:06:38.28 SS.reconfigure :exhale @16:06:38.32 SS.hold @16:06:50.87 Intake.select :close @16:06:51.01 SSV.dial :exhaust @16:06:51.77 Exhaust.select :open @16:06:51.88 SS.seek 0ml @16:06:54.84 PBV.dial between PSV and waste1 @16:06:55.22 PSR.select :air @16:06:55.23 PSV.dial PSR @16:06:56.00 PS.reconfigure :fast @16:06:56.04 PS.seek 2.5ml @16:07:00.70 Exhaust.select :close @16:07:00.81 SSV.dial CBV @16:07:00.83 CBV.dial SSV @16:07:01.61 Allowing 130s for 25.0ml of remaining 250.0ml @16:07:01.63 Intake.select :open @16:07:03.78 Intake @16.0psia, vacuum from 10.0 to 13.0psi @16:07:03.80 SS.reconfigure :inhaling 0.57ml/s @16:07:03.84 SS.seek 25ml @16:07:19.93 PSV.dial PTV @16:07:20.70 delay 5 seconds @16:07:25.72 PSV.dial PSR @16:07:26.48 delay 5 seconds @16:07:31.50 PSV.dial PTV @16:07:32.26 delay 5 seconds @16:07:37.28 PSV.dial :air @16:07:38.05 PS.seek 0ml @16:07:50.06 Stroke completed -- waiting for pressure to equalize ... @16:07:50.07 SS.reconfigure :exhale @16:07:50.11 SS.hold @16:08:01.12 washCycleDA #2 of 3 @16:08:01.13 PSR.select :air @16:08:01.15 PSV.dial PSR @16:08:02.51 Pulling 0.5ml of PSR.wash @16:08:02.52 PSR.select :wash @16:08:02.63 PS.reconfigure :default @16:08:02.68 PS.seek 0.5ml @16:08:04.57 Intake.select :close @16:08:04.72 SSV.dial :exhaust @16:08:05.48 Exhaust.select :open @16:08:05.59 SS.seek 0ml @16:08:08.59 delay 5 seconds @16:08:13.61 PSR.select :air @16:08:13.72 PS.seek 2ml @16:08:14.45 Exhaust.select :close @16:08:14.56 SSV.dial CBV @16:08:14.58 CBV.dial SSV @16:08:15.36 Allowing 130s for 25.0ml of remaining 225.0ml @16:08:15.37 Intake.select :open @16:08:17.53 Intake @16.0psia, vacuum from 10.0 to 13.0psi @16:08:17.54 SS.reconfigure :inhaling 0.57ml/s @16:08:17.59 SS.seek 25ml @16:08:29.84 PTV.dial :puck @16:08:29.85 PSV.dial PTV @16:08:30.59 PBV.dial :waste1 @16:08:31.00 PS.seek 1.5ml @16:08:37.43 delay 3 seconds @16:08:40.45 PBV.dial between PSV and waste1 @16:08:40.83 PS.seek 0ml @16:08:56.93 delay 2:00 @16:09:03.86 Stroke completed -- waiting for pressure to equalize ... @16:09:03.87 SS.reconfigure :exhale @16:09:03.91 SS.hold @16:09:18.27 Intake.select :close @16:09:18.41 SSV.dial :exhaust @16:09:19.17 Exhaust.select :open @16:09:19.28 SS.seek 0ml @16:09:28.17 Exhaust.select :close @16:09:28.27 SSV.dial CBV @16:09:28.32 CBV.dial SSV @16:09:29.05 Allowing 130s for 25.0ml of remaining 200.0ml @16:09:29.06 Intake.select :open @16:09:31.21 Intake @16.1psia, vacuum from 10.0 to 13.0psi @16:09:31.23 SS.reconfigure :inhaling 0.57ml/s @16:09:31.27 SS.seek 25ml @16:10:07.82 Vacuum too high -- waiting up to 40 seconds @16:10:07.83 SS.reconfigure :exhale @16:10:07.87 SS.hold @16:10:14.40 Intake @15.6psia, vacuum from 10.0 to 13.0psi @16:10:14.42 SS.reconfigure :inhaling 0.51ml/s @16:10:14.46 SS.seek 25ml @16:10:25.45 Stroke completed -- waiting for pressure to equalize ... @16:10:25.46 SS.reconfigure :exhale @16:10:25.50 SS.hold @16:10:35.26 Intake.select :close @16:10:35.40 SSV.dial :exhaust @16:10:36.16 Exhaust.select :open @16:10:36.27 SS.seek 0ml @16:10:45.28 Exhaust.select :close @16:10:45.38 SSV.dial CBV @16:10:45.41 CBV.dial SSV @16:10:46.17 Allowing 130s for 25.0ml of remaining 175.0ml @16:10:46.18 Intake.select :open @16:10:48.34 Intake @16.0psia, vacuum from 10.0 to 13.0psi @16:10:48.35 SS.reconfigure :inhaling 0.51ml/s @16:10:48.40 SS.seek 25ml @16:10:56.95 recoverPSRreagent08 @16:10:56.97 PTV.dial :puck @16:10:56.98 PBV.dial :waste1 @16:10:57.33 delay 30 seconds @16:11:27.36 PBV.dial between PSV and waste1 @16:11:27.73 PSR.select :air @16:11:27.74 PSV.dial PSR @16:11:28.51 PS.reconfigure :fast @16:11:28.56 PS.seek 2.5ml @16:11:38.96 Stroke completed -- waiting for pressure to equalize ... @16:11:38.97 SS.reconfigure :exhale @16:11:39.02 SS.hold @16:11:52.30 PSV.dial PTV @16:11:53.07 delay 5 seconds @16:11:53.97 Intake.select :close @16:11:54.11 SSV.dial :exhaust @16:11:54.87 Exhaust.select :open @16:11:54.98 SS.seek 0ml @16:11:58.08 PSV.dial PSR @16:11:58.85 delay 5 seconds @16:12:03.87 PSV.dial PTV @16:12:04.04 Exhaust.select :close @16:12:04.15 SSV.dial CBV @16:12:04.17 CBV.dial SSV @16:12:04.63 delay 5 seconds @16:12:04.95 Allowing 130s for 25.0ml of remaining 150.0ml @16:12:04.96 Intake.select :open @16:12:07.12 Intake @16.1psia, vacuum from 10.0 to 13.0psi @16:12:07.14 SS.reconfigure :inhaling 0.51ml/s @16:12:07.18 SS.seek 25ml @16:12:09.65 PSV.dial :air @16:12:10.53 PS.seek 0ml @16:12:33.71 washCycleDA #3 of 3 @16:12:33.72 PSR.select :air @16:12:33.73 PSV.dial PSR @16:12:35.12 Pulling 0.5ml of PSR.wash @16:12:35.13 PSR.select :wash @16:12:35.24 PS.reconfigure :default @16:12:35.28 PS.seek 0.5ml @16:12:41.18 delay 5 seconds @16:12:46.20 PSR.select :air @16:12:46.31 PS.seek 2ml @16:12:57.53 Vacuum too high -- waiting up to 40 seconds @16:12:57.55 SS.reconfigure :exhale @16:12:57.59 SS.hold @16:13:02.39 PTV.dial :puck @16:13:02.42 PSV.dial PTV @16:13:03.22 PBV.dial :waste1 @16:13:03.65 PS.seek 1.5ml @16:13:05.09 Intake @15.8psia, vacuum from 10.0 to 13.0psi @16:13:05.11 SS.reconfigure :inhaling 0.47ml/s @16:13:05.15 SS.seek 25ml @16:13:05.94 Stroke completed -- waiting for pressure to equalize ... @16:13:05.96 SS.reconfigure :exhale @16:13:06.00 SS.hold @16:13:09.55 delay 3 seconds @16:13:12.56 PBV.dial between PSV and waste1 @16:13:12.93 PS.seek 0ml @16:13:13.40 Intake.select :close @16:13:13.54 SSV.dial :exhaust @16:13:14.29 Exhaust.select :open @16:13:14.40 SS.seek 0ml @16:13:23.23 Exhaust.select :close @16:13:23.33 SSV.dial CBV @16:13:23.35 CBV.dial SSV @16:13:24.12 Allowing 130s for 25.0ml of remaining 125.0ml @16:13:24.13 Intake.select :open @16:13:26.28 Intake @16.2psia, vacuum from 10.0 to 13.0psi @16:13:26.30 SS.reconfigure :inhaling 0.47ml/s @16:13:26.35 SS.seek 25ml @16:13:29.08 delay 2:00 @16:14:22.13 Stroke completed -- waiting for pressure to equalize ... @16:14:22.14 SS.reconfigure :exhale @16:14:22.19 SS.hold @16:14:37.09 Intake.select :close @16:14:37.23 SSV.dial :exhaust @16:14:37.99 Exhaust.select :open @16:14:38.10 SS.seek 0ml @16:14:47.01 Exhaust.select :close @16:14:47.11 SSV.dial CBV @16:14:47.13 CBV.dial SSV @16:14:47.90 Allowing 130s for 25.0ml of remaining 100.0ml @16:14:47.91 Intake.select :open @16:14:50.07 Intake @16.3psia, vacuum from 10.0 to 13.0psi @16:14:50.08 SS.reconfigure :inhaling 0.47ml/s @16:14:50.13 SS.seek 25ml @16:15:29.10 recoverPSRreagent08 @16:15:29.12 PTV.dial :puck @16:15:29.13 PBV.dial :waste1 @16:15:29.49 delay 30 seconds @16:15:45.97 Stroke completed -- waiting for pressure to equalize ... @16:15:45.99 SS.reconfigure :exhale @16:15:46.03 SS.hold @16:15:59.50 PBV.dial between PSV and waste1 @16:15:59.88 PSR.select :air @16:15:59.89 PSV.dial PSR @16:16:00.66 PS.reconfigure :fast @16:16:00.70 PS.seek 2.5ml @16:16:02.23 Intake.select :close @16:16:02.37 SSV.dial :exhaust @16:16:03.13 Exhaust.select :open @16:16:03.24 SS.seek 0ml @16:16:11.99 Exhaust.select :close @16:16:12.10 SSV.dial CBV @16:16:12.13 CBV.dial SSV @16:16:12.88 Allowing 130s for 25.0ml of remaining 75.0ml @16:16:12.89 Intake.select :open @16:16:15.05 Intake @16.2psia, vacuum from 10.0 to 13.0psi @16:16:15.07 SS.reconfigure :inhaling 0.47ml/s @16:16:15.11 SS.seek 25ml @16:16:24.55 PSV.dial PTV @16:16:25.31 delay 5 seconds @16:16:30.33 PSV.dial PSR @16:16:31.08 delay 5 seconds @16:16:36.10 PSV.dial PTV @16:16:36.86 delay 5 seconds @16:16:41.88 PSV.dial :air @16:16:42.76 PS.seek 0ml @16:17:06.11 Gate.power :camera,:ON @16:17:06.12 Mixing DA Substrates @16:17:06.13 PSR.select :air @16:17:06.16 PSV.dial PSR @16:17:06.23 Vacuum too high -- waiting up to 40 seconds @16:17:06.24 SS.reconfigure :exhale @16:17:06.29 SS.hold @16:17:07.89 Pulling 0.25ml of PSR.sub1 @16:17:07.94 PSR.select :sub1 @16:17:08.35 PS.reconfigure :default @16:17:08.51 PS.seek 0.25ml @16:17:11.95 delay 5 seconds @16:17:14.86 Intake @16.0psia, vacuum from 10.0 to 13.0psi @16:17:14.88 SS.reconfigure :inhaling 0.43ml/s @16:17:14.93 SS.seek 25ml @16:17:17.02 Pulling 0.25ml of PSR.sub2 @16:17:17.03 PSR.select :sub2 @16:17:17.15 PS.seek 0.5ml @16:17:20.49 Stroke completed -- waiting for pressure to equalize ... @16:17:20.51 SS.reconfigure :exhale @16:17:20.56 SS.hold @16:17:20.59 delay 5 seconds @16:17:25.61 PSR.select :air @16:17:25.72 PS.seek 2ml @16:17:30.30 Intake.select :close @16:17:30.44 SSV.dial :exhaust @16:17:31.21 Exhaust.select :open @16:17:31.32 SS.seek 0ml @16:17:40.19 Exhaust.select :close @16:17:40.30 SSV.dial CBV @16:17:40.33 CBV.dial SSV @16:17:41.10 Allowing 130s for 25.0ml of remaining 50.0ml @16:17:41.11 Intake.select :open @16:17:41.86 PTV.dial :puck @16:17:41.87 PSV.dial PTV @16:17:42.64 PBV.dial :waste1 @16:17:43.07 PS.seek 1.5ml @16:17:43.26 Intake @16.3psia, vacuum from 10.0 to 13.0psi @16:17:43.28 SS.reconfigure :inhaling 0.43ml/s @16:17:43.33 SS.seek 25ml @16:17:49.01 delay 3 seconds @16:17:52.03 PBV.dial between PSV and waste1 @16:17:52.41 PS.seek 0ml @16:18:08.54 delay 10 seconds @16:18:18.57 recoverPSRreagent08 @16:18:18.58 PTV.dial :puck @16:18:18.59 PBV.dial :waste1 @16:18:18.95 delay 30 seconds @16:18:45.45 Stroke completed -- waiting for pressure to equalize ... @16:18:45.47 SS.reconfigure :exhale @16:18:45.51 SS.hold @16:18:48.97 PBV.dial between PSV and waste1 @16:18:49.34 PSR.select :air @16:18:49.36 PSV.dial PSR @16:18:50.13 PS.reconfigure :fast @16:18:50.19 PS.seek 2.5ml @16:19:01.79 Intake.select :close @16:19:01.94 SSV.dial :exhaust @16:19:02.72 Exhaust.select :open @16:19:02.83 SS.seek 0ml @16:19:11.89 Exhaust.select :close @16:19:12.00 SSV.dial CBV @16:19:12.02 CBV.dial SSV @16:19:12.78 Allowing 130s for 25.0ml of remaining 25.0ml @16:19:12.80 Intake.select :open @16:19:14.29 PSV.dial PTV @16:19:14.95 Intake @16.2psia, vacuum from 10.0 to 13.0psi @16:19:14.97 SS.reconfigure :inhaling 0.43ml/s @16:19:15.01 SS.seek 24.97ml @16:19:15.18 delay 5 seconds @16:19:20.20 PSV.dial PSR @16:19:20.95 delay 5 seconds @16:19:25.97 PSV.dial PTV @16:19:26.73 delay 5 seconds @16:19:31.75 PSV.dial :air @16:19:32.64 PS.seek 0ml @16:19:56.29 Additional air clear @16:19:56.30 PSR.select :air @16:19:56.32 PSV.dial PSR @16:19:57.72 Pulling 2ml of PSR.air @16:19:57.73 PSR.select :air @16:19:57.75 PS.reconfigure :default @16:19:57.79 PS.seek 2ml @16:20:16.59 Stroke completed -- waiting for pressure to equalize ... @16:20:17.05 SS.reconfigure :exhale @16:20:17.17 SS.hold @16:20:19.18 delay 5 seconds @16:20:24.20 PTV.dial :puck @16:20:24.21 PSV.dial PTV @16:20:25.10 PBV.dial :waste1 @16:20:25.52 PS.seek 1.5ml @16:20:31.50 delay 3 seconds @16:20:34.52 PBV.dial between PSV and waste1 @16:20:34.89 delay 1 second @16:20:35.50 Intake.select :close @16:20:35.64 SSV.dial :exhaust @16:20:35.91 PSV.dial :waste1 @16:20:36.41 Exhaust.select :open @16:20:36.52 SS.seek 0ml @16:20:36.97 PS.seek 0ml @16:20:45.56 Exhaust.select :close @16:20:45.67 Sampled 1000.0ml @16:20:45.69 Drying puck before fixing for 20:00 CBV.dial :waste2b @16:20:46.46 delay 1:00 @16:20:53.22 Processing Puck Heater at 28C before imaging @16:20:53.23 PH.coast @16:20:53.35 Imaging da puck in Processing Clamp while storing it to tube 3 @16:20:53.51 SC.reconfigure :tight @16:20:53.55 SC.seek 3 @16:20:57.15 SC.configure :limp @16:20:57.34 Elbow.reconfigure :tight @16:20:57.39 Elbow.seek 3 @16:20:59.09 Forearm.reconfigure :tight @16:20:59.14 Forearm.seek 3 + 450 counts @16:21:00.20 SE.reconfigure :pick @16:21:00.25 SE.seek :beamBreak @16:21:03.15 SE.configure :default @16:21:03.29 Elbow.reconfigure :limp @16:21:03.34 Forearm.reconfigure :loose @16:21:03.38 Forearm.seek 3 @16:21:03.65 Elbow.hold @16:21:03.71 Elbow.reconfigure :tight @16:21:03.80 SE.seek 3.16 Pucks below Top Plate @16:21:04.25 Forearm.force -20 @16:21:04.29 Forearm.seek 3 + 263 counts @16:21:04.56 Forearm.reconfigure :tight @16:21:04.61 Forearm.seek 3 + 450 counts @16:21:04.79 SE.seek 4.16 Pucks below Top Plate @16:21:05.27 Forearm.reconfigure :loose @16:21:05.31 Forearm.seek :retract @16:21:06.24 Elbow.seek PC @16:21:08.74 Elbow.reconfigure :loose @16:21:08.83 Forearm.seek PC @16:21:09.79 Hand.close @16:21:10.44 PC.seek :open @16:21:15.20 Forearm.seek :retract @16:21:16.17 Elbow.reconfigure :tight @16:21:16.22 Elbow.seek 3 @16:21:18.84 Forearm.reconfigure :tight @16:21:18.89 Forearm.seek 3 + 450 counts @16:21:19.89 Hand.open @16:21:20.50 SE.seek 3.81 Pucks below Top Plate @16:21:20.75 SE.reconfigure :seal @16:21:20.79 SE.seek 2.85 Pucks below Top Plate @16:21:21.36 Lights, camera, ACTION! @16:21:21.39 PuckCamera: snap -bin=2,2 -auto /var/log/esp/da23feb2116h850ml.tif @16:21:40.84 Waiting >20 seconds for da to relinquish FlushPuck @16:21:46.27 SX HX9/16 (USB:18): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 21.56 seconds /var/log/esp/da23feb2116h850ml.tif: TIFF Upload Complete @16:21:46.29 PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da23feb2116h850ml15s.tif @16:21:46.48 CSV.dial :air @16:21:46.98 CS.reconfigure :fast @16:21:47.03 CS.seek 10ml @16:22:01.91 SX HX9/16 (USB:18): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15 seconds /var/log/esp/midres/da23feb2116h850ml15s.tif: TIFF Upload Complete @16:22:01.93 PuckCamera: snap -bin=2,2 20.0 /var/log/esp/midres/da23feb2116h850ml20s.tif @16:22:11.62 Pumping wcr puck from tube 4 up to 35psi... @16:22:11.64 CBV.dial SSV @16:22:12.97 CBV.dial between CSV and waste2b @16:22:13.94 CSV.dial between air and CTV @16:22:14.28 CS.seek 7.017ml @16:22:21.93 CTV.dial CSV,avoiding: :air @16:22:21.96 CSV.dial CTV @16:22:22.58 SX HX9/16 (USB:18): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 20 seconds /var/log/esp/midres/da23feb2116h850ml20s.tif: TIFF Upload Complete @16:22:22.60 Gate.power :camera,:OFF @16:22:22.62 DAfans.email "21.56 sec auto-exposure",Subject: "da23feb2116h850ml images!" @16:22:22.87 CS.reconfigure :pressurize @16:22:22.94 CS.seek 0ml @16:22:22.97 Elbow.seek 3 @16:22:23.48 Forearm.seek 3 + 450 counts @16:22:23.55 SE.reconfigure :gently @16:22:23.60 SE.seek 3.56 Pucks below Top Plate @16:22:24.28 Elbow.reconfigure :limp @16:22:24.35 Forearm.reconfigure :loose @16:22:24.40 Forearm.seek 3 @16:22:24.66 Elbow.hold @16:22:24.73 Elbow.reconfigure :tight @16:22:24.78 SE.reconfigure :default @16:22:24.83 SE.seek 3.75 Pucks below Top Plate @16:22:25.22 Forearm.force -20 @16:22:25.26 Forearm.seek 3 + 274 counts @16:22:25.63 Forearm.reconfigure :tight @16:22:25.67 Forearm.seek 3 + 450 counts @16:22:25.94 SE.seek :down @16:22:27.08 Forearm.reconfigure :loose @16:22:27.13 Forearm.seek :retract @16:22:28.40 SE.stop @16:22:28.65 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @16:22:29.01 Elbow.seek FlushPuck::Garage @16:22:30.95 Elbow.reconfigure :loose @16:22:31.13 Forearm.seek :garage @16:22:32.14 Hand.close @16:22:33.07 Forearm.seek :retract @16:22:34.08 Elbow.reconfigure :tight @16:22:34.17 Elbow.seek PC @16:22:35.50 Elbow.reconfigure :loose @16:22:35.69 Forearm.reconfigure :tight @16:22:35.78 Forearm.seek PC @16:22:36.01 CBV.dial :waste2b @16:22:36.43 CS.reconfigure :default @16:22:36.51 CS.seek 0ml @16:22:37.35 PC.seek :closed @16:22:42.10 Processing Clamp onSpring/offSpring for 16/26 samples, on/off torque 1.58 @16:22:42.25 Hand.open @16:22:42.76 delay 1:00 @16:22:43.08 Forearm.reconfigure :loose @16:22:43.15 Forearm.seek :retract @16:22:44.13 PSR.select :air @16:22:44.17 PSV.dial PSR @16:22:44.78 Pulling 0.5ml of PSR.flush @16:22:44.80 PSR.select :flush @16:22:44.98 PS.seek 0.5ml @16:22:51.03 delay 5 seconds @16:22:56.06 PSR.select :air @16:22:56.21 PS.seek 2.5ml @16:22:59.47 upsync @16:23:17.41 delay 1 second @16:23:18.43 PRV.dial :waste2,avoiding: :ab2 @16:23:20.30 PTV.dial :PRVmixing @16:23:21.62 PSV.dial :mixloop @16:23:22.66 PS.seek :empty @16:23:42.80 CTV.dial :air,avoiding: :intake @16:23:44.13 Fix #1 of 2 (with RNAlater) for 10:00 @16:23:44.14 CSV.dial CSR @16:23:44.63 Pulling 2ml of CSR.RNAlater @16:23:44.64 CSR.select :RNAlater @16:23:44.75 CS.seek 2ml @16:23:48.97 PSR.select :air @16:23:49.03 PSV.dial PSR @16:23:50.17 Pulling 0.5ml of PSR.flush @16:23:50.18 PSR.select :flush @16:23:50.30 PS.seek 0.5ml @16:23:50.71 delay 5 seconds @16:23:55.73 CSR.select :air @16:23:55.83 CS.reconfigure :fast @16:23:55.88 CS.seek 10ml @16:23:56.23 delay 5 seconds @16:24:01.24 PSR.select :air @16:24:01.36 PS.seek 2.5ml @16:24:15.45 Pumping wcr puck from tube 4 up to 35psi... @16:24:15.47 CBV.dial SSV @16:24:16.77 CBV.dial between CSV and waste2b @16:24:17.71 CSV.dial between air and CTV @16:24:18.32 CS.seek 7.005ml @16:24:22.60 delay 1 second @16:24:23.62 PBV.dial :waste1 @16:24:23.99 PTV.dial :mixing @16:24:24.74 PSV.dial :mixloop @16:24:25.81 PS.seek :empty @16:24:25.99 CTV.dial CSV,avoiding: :air @16:24:26.01 CSV.dial CTV @16:24:27.43 CS.reconfigure :pressurize @16:24:27.48 CS.seek 0ml @16:24:45.89 CSV.dial :air @16:24:46.35 CS.reconfigure :fast @16:24:46.39 CS.seek 10ml @16:24:52.16 PSV.dial PSR @16:24:53.22 PSR.select :flush @16:24:53.34 PS.seek 1ml @16:25:04.44 PSR.select :air @16:25:04.56 PS.seek 2.5ml @16:25:10.57 CSV.dial between air and CTV @16:25:10.90 CS.seek 3.954ml @16:25:20.75 PSV.dial PTV @16:25:21.52 PTV.dial :puck @16:25:22.25 PBV.dial :waste1 @16:25:22.27 PS.seek 0ml @16:25:25.24 CTV.dial CSV,avoiding: :air @16:25:25.26 CSV.dial CTV @16:25:25.57 CS.reconfigure :pressurize @16:25:25.61 CS.seek 0ml @16:25:36.20 CSV.dial :air @16:25:36.66 CS.reconfigure :fast @16:25:36.71 CS.seek 10ml @16:25:48.63 PSV.dial PSR @16:25:49.38 PSR.select :flush @16:25:49.96 PS.seek 0.5ml @16:25:55.88 PSR.select :air @16:25:55.99 PS.seek 2.5ml @16:26:00.85 CSV.dial between air and CTV @16:26:01.18 CS.seek 6.178ml @16:26:10.58 CTV.dial CSV,avoiding: :air @16:26:10.60 CSV.dial CTV @16:26:10.91 CS.reconfigure :pressurize @16:26:10.96 CS.seek 0ml @16:26:17.31 PSV.dial PTV @16:26:18.07 PBV.dial PSV @16:26:18.64 PS.seek 0ml @16:26:26.44 delay 10:00 @16:26:45.16 PSV.dial PBV @16:26:45.63 PS.seek 2.5ml @16:27:11.91 PSV.dial :waste1 @16:27:12.65 PS.seek 0ml @16:27:39.00 PSV.dial PSR @16:27:39.47 PSR.select :air @16:27:39.49 PS.seek 2.5ml @16:28:05.84 PSV.dial PTV @16:28:06.70 PBV.dial PSV @16:28:06.72 PS.seek 0ml @16:28:33.06 PSV.dial PBV @16:28:33.52 PS.seek 2.5ml @16:29:00.00 PSV.dial :waste1 @16:29:00.74 PS.seek 0ml @16:29:27.12 PSV.dial PSR @16:29:27.59 PSR.select :flush @16:29:27.71 PS.seek 1ml @16:29:38.81 PSR.select :air @16:29:38.92 PS.seek 2.5ml @16:29:55.12 PSV.dial PTV @16:29:55.86 PTV.dial :puck @16:29:55.87 PBV.dial :waste1 @16:29:56.43 PS.seek 0ml @16:30:22.81 PSV.dial PSR @16:30:23.56 PSR.select :flush @16:30:23.68 PS.seek 0.5ml @16:30:29.56 PSR.select :air @16:30:29.67 PS.seek 2.5ml @16:30:51.03 PSV.dial PTV @16:30:51.78 PBV.dial PSV @16:30:52.46 PS.seek 0ml @16:31:18.78 PSV.dial PBV @16:31:19.25 PS.seek 2.5ml @16:31:45.59 PSV.dial :waste1 @16:31:46.35 PS.seek 0ml @16:32:12.68 PSV.dial PSR @16:32:13.15 PSR.select :air @16:32:13.18 PS.seek 2.5ml @16:32:39.56 PSV.dial PTV @16:32:40.29 PBV.dial PSV @16:32:40.31 PS.seek 0ml @16:33:06.87 PSV.dial PBV @16:33:07.35 PS.seek 2.5ml @16:33:33.74 PSV.dial :waste1 @16:33:34.47 PS.seek 0ml @16:34:00.78 Garaging FlushPuck in Processing Clamp @16:34:00.85 Elbow.reconfigure :tight @16:34:00.89 Elbow.seek PC @16:34:00.99 Elbow.reconfigure :loose @16:34:01.08 Forearm.seek PC @16:34:02.05 Hand.close @16:34:02.68 PC.seek :open @16:34:07.38 Forearm.seek :retract @16:34:08.33 Elbow.reconfigure :tight @16:34:08.38 Elbow.seek :garage @16:34:09.71 Elbow.reconfigure :loose @16:34:09.80 Forearm.reconfigure :tight @16:34:09.85 Forearm.seek :garage @16:34:10.85 Hand.open @16:34:11.48 Forearm.reconfigure :loose @16:34:11.53 Forearm.seek :retract @16:36:26.46 CBV.dial :waste2b @16:36:26.79 CS.reconfigure :default @16:36:26.83 CS.seek 0ml @16:36:28.84 delay 1:00 @16:37:28.87 CTV.dial :air,avoiding: :intake @16:37:30.21 Fix #2 of 2 (with RNAlater) for 10:00 @16:37:30.22 CSV.dial CSR @16:37:30.71 Pulling 2ml of CSR.RNAlater @16:37:30.72 CSR.select :RNAlater @16:37:30.83 CS.seek 2ml @16:37:36.84 delay 5 seconds @16:37:41.86 CSR.select :air @16:37:41.97 CS.reconfigure :fast @16:37:42.02 CS.seek 10ml @16:38:01.63 Pumping wcr puck from tube 4 up to 35psi... @16:38:01.64 CBV.dial SSV @16:38:02.94 CBV.dial between CSV and waste2b @16:38:03.89 CSV.dial between air and CTV @16:38:04.49 CS.seek 7.049ml @16:38:11.94 CTV.dial CSV,avoiding: :air @16:38:11.97 CSV.dial CTV @16:38:13.33 CS.reconfigure :pressurize @16:38:13.38 CS.seek 0ml @16:38:29.05 delay 10:00 @16:48:29.07 CBV.dial :waste2b @16:48:29.40 CS.reconfigure :default @16:48:29.45 CS.seek 0ml @16:48:32.99 delay 1:00 @16:49:33.02 CTV.dial :air,avoiding: :intake @16:49:34.41 Storing wcr puck in Collection Clamp to tube 3 @16:49:34.77 Elbow.reconfigure :tight @16:49:34.82 Elbow.seek 3 @16:49:36.68 Forearm.reconfigure :tight @16:49:36.73 Forearm.seek 3 + 450 counts @16:49:37.75 SE.reconfigure :pick @16:49:37.81 SE.seek :beamBreak @16:49:40.66 SE.configure :default @16:49:40.81 Elbow.reconfigure :limp @16:49:40.85 Forearm.reconfigure :loose @16:49:40.90 Forearm.seek 3 @16:49:41.16 Elbow.hold @16:49:41.23 Elbow.reconfigure :tight @16:49:41.32 SE.seek 4.17 Pucks below Top Plate @16:49:41.77 Forearm.force -20 @16:49:41.81 Forearm.seek 3 + 299 counts @16:49:42.09 Forearm.reconfigure :tight @16:49:42.13 Forearm.seek 3 + 450 counts @16:49:42.33 SE.seek 5.17 Pucks below Top Plate @16:49:42.82 Forearm.reconfigure :loose @16:49:42.87 Forearm.seek :retract @16:49:43.81 Elbow.seek CC @16:49:45.44 Elbow.reconfigure :loose @16:49:45.53 Forearm.seek CC @16:49:46.47 Hand.close @16:49:47.04 CC.seek :open @16:49:51.77 Forearm.seek :retract @16:49:52.74 Elbow.reconfigure :tight @16:49:52.79 Elbow.seek 3 @16:49:54.57 Elbow.reconfigure :limp @16:49:54.62 Forearm.seek 3 @16:49:55.65 Elbow.hold @16:49:55.71 Elbow.reconfigure :tight @16:49:55.76 Hand.open @16:49:56.91 Forearm.force -20 @16:49:56.94 Forearm.seek 3 + 314 counts @16:49:57.24 Forearm.reconfigure :tight @16:49:57.29 Forearm.seek 3 + 450 counts @16:49:57.56 SE.seek :down @16:49:58.68 Forearm.reconfigure :loose @16:49:58.73 Forearm.seek :retract @16:49:59.89 SE.stop @16:49:59.93
Starting finalPurge @16:49:59.94 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @16:50:00.12 Elbow.seek FlushPuck::Garage @16:50:01.97 Elbow.reconfigure :loose @16:50:02.06 Forearm.seek :garage @16:50:03.01 Hand.close @16:50:03.77 Forearm.seek :retract @16:50:04.74 Elbow.reconfigure :tight @16:50:04.79 Elbow.seek CC @16:50:07.73 Elbow.reconfigure :loose @16:50:07.82 Forearm.reconfigure :tight @16:50:07.87 Forearm.seek CC @16:50:09.07 CC.seek :closed @16:50:13.58 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.24 @16:50:13.64 Hand.open @16:50:14.34 Forearm.reconfigure :loose @16:50:14.39 Forearm.seek :retract @16:50:15.40 Flushing the Collection Valve::Manifold @16:50:15.52 CSV.dial CSR @16:50:15.99 Pulling 5ml of CSR.flush @16:50:16.00 CSR.select :flush @16:50:16.11 CS.seek 5ml @16:50:29.71 delay 5 seconds @16:50:34.72 CSR.select :air @16:50:34.87 CS.reconfigure :fast @16:50:34.91 CS.seek 7.5ml @16:50:41.64 CSV.dial :waste @16:50:42.91 CS.reconfigure :default @16:50:42.95 CS.seek 0ml @16:51:03.02 Flushing lower manifold's waste1 port @16:51:03.13 CSV.dial :flush @16:51:03.57 CS.reconfigure :fast @16:51:03.61 CS.seek 5ml @16:51:16.42 delay 5 seconds @16:51:21.44 CSV.dial :air @16:51:22.06 CS.seek 7ml @16:51:27.52 CSV.dial CTV @16:51:27.54
CTV.dial CSV @16:51:28.92 CBV.dial :waste1 @16:51:29.68 CS.seek 0ml @16:51:46.46 Flushing lower manifold's waste2 port @16:51:46.57 CSV.dial :flush @16:51:47.29 CS.seek 5ml @16:52:00.14 delay 5 seconds @16:52:05.16 CSV.dial :air @16:52:05.67 CS.seek 7ml @16:52:11.24 CSV.dial CTV @16:52:11.28
CTV.dial CSV @16:52:11.69 CBV.dial :waste2 @16:52:12.73 CS.seek 0ml @16:52:29.24 Flushing lower manifold's waste2b port @16:52:29.35 CSV.dial :flush @16:52:30.08 CS.seek 5ml @16:52:42.77 delay 5 seconds @16:52:47.78 CSV.dial :air @16:52:48.29 CS.seek 7.001ml @16:52:53.77 CSV.dial CTV @16:52:53.81
CTV.dial CSV @16:52:54.22 CBV.dial :waste2b @16:52:55.27 CS.seek 0ml @16:53:11.70 Disinfecting external Intake with kill @16:53:11.82 CSV.dial CSR @16:53:12.40 Pulling 8ml of CSR.kill @16:53:12.41 CSR.select :kill @16:53:12.52 CS.seek 8ml @16:53:32.21 delay 5 seconds @16:53:37.22 CSR.select :air @16:53:37.33 CS.seek 10ml @16:53:42.80 CSV.dial CBV @16:53:42.82
CBV.dial CSV @16:53:43.86 CTV.dial :intake @16:53:44.64 Intake.select :open @16:53:44.75 CS.seek 2ml @16:54:03.73 Intake.select :close @16:54:03.83 CTV.dial :air @16:54:04.58 CS.reconfigure :default @16:54:04.62 CS.seek 0ml @16:54:10.58 CSV.dial CSR @16:54:11.74 Pulling 5ml of CSR.flush @16:54:11.75 CSR.select :flush @16:54:11.86 CS.seek 5ml @16:54:25.64 delay 5 seconds @16:54:30.66 CSR.select :air @16:54:30.77 CS.seek 10ml @16:54:44.29 CSV.dial CBV @16:54:45.27 CS.seek 0ml @16:55:11.64 Garaging FlushPuck in Collection Clamp @16:55:11.71 Elbow.reconfigure :tight @16:55:11.75 Elbow.seek CC @16:55:12.04 Elbow.reconfigure :loose @16:55:12.13 Forearm.seek CC @16:55:13.06 Hand.close @16:55:13.66 CC.seek :open @16:55:18.28 Forearm.seek :retract @16:55:19.27 Elbow.reconfigure :tight @16:55:19.31 Elbow.seek :garage @16:55:22.20 Elbow.reconfigure :loose @16:55:22.28 Forearm.reconfigure :tight @16:55:22.32 Forearm.seek :garage @16:55:23.35 Hand.open @16:55:23.97 Forearm.reconfigure :loose @16:55:24.01 Forearm.seek :retract @16:55:25.00 Completed [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] begun 13:55:00 @16:55:25.05 Asleep after completing DA_WCR_022123 assays @16:55:25.07 GoodNews.email "{1 => 33,\n 2 => 33,\n 3 => 6,\n 4 => 27,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@16:54:11, 17% flow, 18.6C, 37% humidity, 14.7psia, 13.661V, 0.659A, 0.647A avg, 9.00W\nSampled 1000.0ml",Subject: "Asleep after completing DA_WCR_022123 assays" @16:55:30.17 upsync @15:10:27.08UTC28-Feb-23
ESP2 v3.41 I2C Gateway v3.20 for core bus @15:10:29.63 Configuration for short puck jake 1/11/21 by brent@mbari.org /home/esp/esp2/type/shallow/jake/configure.rb @15:10:33.49 Polling Can every 10:00 @15:10:33.70 Cleanly exited at 15:08:02UTC28-Feb-23 @15:10:33.74 Loading /home/esp/esp2/mission/DA_WCR_022823.rb mission @15:10:33.79 GoodNews.email "Starting DA_WCR_022823 w/pucks in tube 4\n{1 => 33,\n 2 => 33,\n 3 => 6,\n 4 => 27,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@15:10:33, 16% flow, 18.9C, 37% humidity, 14.7psia, 13.617V, 0.272A, 0.260A avg, 3.71W",Subject: "DA_WCR_022823 begun" @15:10:33.81
Preparing to sleep until + 00:00:05 @15:10:33.86 Asleep til + 00:00:05 @15:10:33.87 Continuing at 15:10 @15:10:34.16 Gate.power :core,:OFF @15:10:34.18 Gate.power :raw,:OFF Shutdown @15:10:38.76
Powering core bus @15:10:39.03 GoodNews.email "Then, in tube 4: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2/mission/DA_WCR_022823.rb:14\n{1 => 33,\n 2 => 33,\n 3 => 6,\n 4 => 27,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@15:10:33, 16% flow, 18.9C, 37% humidity, 14.7psia, 13.617V, 0.272A, 0.260A avg, 3.71W",Subject: "Asleep until 15:10" @15:10:40.94 upsync @15:10:44.20
Gate.power :raw,:ON @15:10:44.66 upsync @15:10:45.10
CS.configure :default @15:10:45.19 PS.configure :default @15:10:45.27 SS.configure :default @15:10:45.36 CC.configure :default @15:10:45.45 PC.configure :default @15:10:45.56 Forearm.configure :loose @15:10:45.64 Elbow.configure :loose @15:10:45.75 SC.configure :loose @15:10:45.83 SE.configure :default @15:10:45.88 CH.coast @15:10:45.93 CH.configure :default @15:10:45.96 PH.coast @15:10:46.01 PH.configure :default @15:10:46.05 All dwarf controllers running firmware version 3.85 @15:10:46.15 Valve.reset @15:10:46.34 Intake.select :close @15:10:46.40 Exhaust.select :close @15:10:46.45 CTV.dial :air @15:10:46.55 PSV.dial :air @15:10:46.62 CSV.dial :waste1 @15:10:46.65
Forearm.reconfigure :loose @15:10:46.72 Forearm.home.seek :retract @15:10:47.12 SSV.dial :spit,avoiding: :exhaust @15:10:47.43 Hand.close @15:10:47.46
Elbow.jog 1500 @15:10:47.75 PS.reconfigure :default @15:10:47.79 PS.home.seek 0ml @15:10:47.94 CS.reconfigure :fast @15:10:47.98 SS.reconfigure :default @15:10:48.05 CS.home.seek 0ml @15:10:48.09 SS.home.seek 0ml @15:10:48.19
Elbow.reconfigure :loose @15:10:48.23 Elbow.home.seek :clear @15:10:51.47 SE.reconfigure :default @15:10:51.51 SE.home.seek :down @15:10:52.10 SC.reconfigure :tight @15:10:52.14 SC.home.seek :initial @15:10:56.03 PS.stop @15:11:20.44
SC.configure :limp @15:11:20.49 Forearm.seek :retract @15:11:20.54 Elbow.seek :hammer @15:11:21.59 Forearm.reconfigure :tight @15:11:21.63 Forearm.seek retracted @15:11:21.84 PC.reconfigure :default @15:11:21.86
CC.reconfigure :default @15:11:21.95 PC.home.seek :open @15:11:21.96
CC.home.seek :open @15:11:25.48 Phase [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] @15:11:25.49 Notice.email "At 15:11:25 began in tube 4: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2/mission/DA_WCR_022823.rb:14\n{1 => 33,\n 2 => 33,\n 3 => 6,\n 4 => 27,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@15:11:25, 16% flow, 19.0C, 37% humidity, 14.8psia, 13.715V, 0.302A, 0.284A avg, 4.15W",Subject: "Started DA_WCR_022823 phase initialPurge; da [1000, 1000] #da and wsr are run simultanelously" @15:11:25.51
Starting initialPurge @15:11:25.54 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @15:11:25.60 Hand.open @15:11:26.31 Elbow.reconfigure :tight @15:11:26.37 Elbow.seek FlushPuck::Garage @15:11:28.21 Elbow.reconfigure :loose @15:11:28.29 Forearm.reconfigure :loose @15:11:28.34 Forearm.seek :garage @15:11:29.26 Hand.close @15:11:29.96 Forearm.seek :retract @15:11:30.95 Elbow.reconfigure :tight @15:11:31.00 Elbow.seek CC @15:11:31.21 upsync @15:11:33.96
Elbow.reconfigure :loose @15:11:34.05 Forearm.reconfigure :tight @15:11:34.09 Forearm.seek CC @15:11:35.34 CC.seek :closed @15:11:39.95 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.34 @15:11:40.02 Hand.open @15:11:40.63 Forearm.reconfigure :loose @15:11:40.67 Forearm.seek :retract @15:11:41.62 Flushing the Collection Valve::Manifold @15:11:41.74 CSV.dial CSR @15:11:43.06 Pulling 5ml of CSR.flush @15:11:43.07 CSR.select :flush @15:11:43.18 CS.reconfigure :default @15:11:43.22 CS.seek 5ml @15:11:56.80 delay 5 seconds @15:12:01.82 CSR.select :air @15:12:01.97 CS.reconfigure :fast @15:12:02.01 CS.seek 7.5ml @15:12:08.86 CSV.dial :waste @15:12:10.13 CS.reconfigure :default @15:12:10.17 CS.seek 0ml @15:12:30.18 Purging external Intake @15:12:30.29 CSV.dial :flush @15:12:30.85 CS.reconfigure :fast @15:12:30.90 CS.seek 10ml @15:12:55.52 delay 5 seconds @15:13:00.54 CSV.dial CBV @15:13:00.57
CBV.dial CSV @15:13:01.30 CTV.dial :intake @15:13:02.10 Intake.select :open @15:13:02.20 CS.seek 0ml @15:13:25.89 Intake.select :close @15:13:25.99 CTV.dial :air @15:13:26.79 Flushing upper manifold @15:13:26.90 CSV.dial :flush @15:13:27.63 CS.seek 5ml @15:13:40.36 delay 5 seconds @15:13:45.38 CSV.dial :air @15:13:46.00 CS.seek 7ml @15:13:51.61 CSV.dial CBV @15:13:51.63
CBV.dial CSV @15:13:52.66 CTV.dial :air @15:13:52.68 CS.seek 0ml @15:14:09.42 delay 7 seconds @15:14:16.45 CS.seek 3ml @15:14:24.55 CSV.dial :waste @15:14:24.99 CS.seek 0ml @15:14:32.77 CSV.dial :flush @15:14:33.23 CS.seek 3ml @15:14:41.36 delay 5 seconds @15:14:46.38 CSV.dial :air @15:14:46.87 CS.seek 5.001ml @15:14:52.58 CSV.dial :air @15:14:52.60 CS.seek 0ml @15:15:04.74 delay 7 seconds @15:15:11.76 CS.seek 3ml @15:15:19.86 CSV.dial :waste @15:15:20.59 CS.seek 0ml @15:15:28.27 Garaging FlushPuck in Collection Clamp @15:15:28.33 Elbow.reconfigure :tight @15:15:28.38 Elbow.seek CC @15:15:28.64 Elbow.reconfigure :loose @15:15:28.72 Forearm.seek CC @15:15:29.69 Hand.close @15:15:30.32 CC.seek :open @15:15:34.97 Forearm.seek :retract @15:15:35.94 Elbow.reconfigure :tight @15:15:35.99 Elbow.seek :garage @15:15:38.87 Elbow.reconfigure :loose @15:15:38.96 Forearm.reconfigure :tight @15:15:39.00 Forearm.seek :garage @15:15:39.95 Hand.open @15:15:40.70 Forearm.reconfigure :loose @15:15:40.74 Forearm.seek :retract @15:15:41.70 da sampling at most 1000ml, wcr at most 1000ml @15:15:41.73 Loading sh1 puck from tube 4 into Collection Clamp @15:15:41.74 DAfans.email "Started da in tube 4 at 15:15:41UTC28-Feb-23\n{1 => 33,\n 2 => 33,\n 3 => 6,\n 4 => 27,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@15:11:25, 16% flow, 19.0C, 37% humidity, 14.8psia, 13.715V, 0.302A, 0.284A avg, 4.15W",Subject: "da sampling at most 1000ml, wcr at most 1000ml" @15:15:42.22
Elbow.reconfigure :tight @15:15:42.27 Elbow.seek 4 @15:15:44.21 Forearm.reconfigure :tight @15:15:44.25 Forearm.seek 4 + 450 counts @15:15:45.31 SE.reconfigure :pick @15:15:45.37 SE.seek :beamBreak @15:15:46.77 SE.configure :default @15:15:46.96 Elbow.seek 4 @15:15:47.14 Forearm.seek 4 + 300 counts @15:15:47.33 Forearm.reconfigure :loose @15:15:47.38 Hand.close @15:15:47.53 upsync @15:15:47.96 SE.seek :down @15:15:48.16
Forearm.seek :retract @15:15:48.98 SE.stop @15:15:49.09
Elbow.seek CC @15:15:50.73 Elbow.reconfigure :loose @15:15:50.82 Forearm.reconfigure :tight @15:15:50.87 Forearm.seek CC @15:15:52.04 CC.seek :closed @15:15:56.59 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.32 @15:15:56.60 Hand.open @15:15:57.25 Forearm.reconfigure :loose @15:15:57.29 Forearm.seek :retract @15:15:58.24 collectSample 1000ml w/bubble point of 24psi @15:15:58.25 Priming 7ml @15:15:58.26 Intake.select :close @15:15:58.27 Exhaust.select :close @15:15:58.28 SS.hold @15:15:58.48 Calibrating pressure and temperature sensors @15:15:58.50 CTV.dial :air @15:15:58.52 SSV.dial :spit,avoiding: :exhaust @15:15:58.54 SS.reconfigure :fast @15:15:58.58 SS.seek 5ml @15:16:01.62 SSV.dial CBV @15:16:01.65
CBV.dial SSV @15:16:02.97 delay 3.8 seconds @15:16:06.89 SSV.dial :spit @15:16:08.21 SS.seek 0ml @15:16:10.81 SSV.dial CBV @15:16:12.11 CTV.dial :intake @15:16:12.90 SSV.dial CBV @15:16:12.92
CBV.dial SSV @15:16:12.93 Allowing 40s for 7.0ml of remaining 7.0ml @15:16:12.94 Intake.select :open @15:16:15.09 Intake @15.1psia, vacuum from 9.6 to 12.6psi @15:16:15.11 SS.reconfigure :inhaling 2.20ml/s @15:16:15.15 SS.seek 7ml @15:16:22.54 Stroke completed -- waiting for pressure to equalize ... @15:16:22.55 SS.reconfigure :exhale @15:16:22.59 SS.hold @15:16:23.45 Intake.select :close @15:16:23.59 SSV.dial :exhaust @15:16:24.33 Exhaust.select :open @15:16:24.44 SS.seek 0ml @15:16:28.49 Exhaust.select :close @15:16:28.60 Sampling 1000.0ml @15:16:28.61 Intake.select :close @15:16:28.62 Exhaust.select :close @15:16:28.63 SS.reconfigure :default @15:16:28.67 SS.hold @15:16:28.85 CTV.dial :intake @15:16:28.86 SSV.dial CBV @15:16:28.89
CBV.dial SSV @15:16:29.65 Allowing 130s for 25.0ml of remaining 1000.0ml @15:16:29.66 Intake.select :open @15:16:31.81 Intake @15.1psia, vacuum from 9.6 to 12.6psi @15:16:31.83 SS.reconfigure :inhaling 2.20ml/s @15:16:31.87 SS.seek 25ml @15:16:42.58 Vacuum too high -- waiting up to 40 seconds @15:16:42.59 SS.reconfigure :exhale @15:16:42.63 SS.hold @15:16:47.11 Intake @13.9psia, vacuum from 8.4 to 11.4psi @15:16:47.13 SS.reconfigure :inhaling 1.98ml/s @15:16:47.17 SS.seek 25ml @15:16:52.60 Stroke completed -- waiting for pressure to equalize ... @15:16:52.61 SS.reconfigure :exhale @15:16:52.65 SS.hold @15:16:57.42 Intake.select :close @15:16:57.56 SSV.dial :exhaust @15:16:58.31 Exhaust.select :open @15:16:58.41 SS.seek 0ml @15:17:07.46 Exhaust.select :close @15:17:07.56 SSV.dial CBV @15:17:07.59
CBV.dial SSV @15:17:08.36 Allowing 130s for 25.0ml of remaining 975.0ml @15:17:08.37 Intake.select :open @15:17:10.52 Intake @15.8psia, vacuum from 10.0 to 13.0psi @15:17:10.53 SS.reconfigure :inhaling 1.98ml/s @15:17:10.58 SS.seek 25ml @15:17:19.55 Vacuum too high -- waiting up to 40 seconds @15:17:19.56 SS.reconfigure :exhale @15:17:19.60 SS.hold @15:17:21.89 Intake @13.7psia, vacuum from 8.2 to 11.2psi @15:17:21.91 SS.reconfigure :inhaling 1.79ml/s @15:17:21.95 SS.seek 25ml @15:17:30.15 Stroke completed -- waiting for pressure to equalize ... @15:17:30.16 SS.reconfigure :exhale @15:17:30.20 SS.hold @15:17:37.58 Intake.select :close @15:17:37.71 SSV.dial :exhaust @15:17:38.46 Exhaust.select :open @15:17:38.57 SS.seek 0ml @15:17:47.34 Exhaust.select :close @15:17:47.44 SSV.dial CBV @15:17:47.46
CBV.dial SSV @15:17:48.23 Allowing 130s for 25.0ml of remaining 950.0ml @15:17:48.24 Intake.select :open @15:17:50.41 Intake @16.1psia, vacuum from 10.0 to 13.0psi @15:17:50.42 SS.reconfigure :inhaling 1.79ml/s @15:17:50.47 SS.seek 25ml @15:17:59.24 Vacuum too high -- waiting up to 40 seconds @15:17:59.25 SS.reconfigure :exhale @15:17:59.29 SS.hold @15:18:01.52 Intake @14.0psia, vacuum from 8.5 to 11.5psi @15:18:01.54 SS.reconfigure :inhaling 1.61ml/s @15:18:01.58 SS.seek 25ml @15:18:11.51 Stroke completed -- waiting for pressure to equalize ... @15:18:11.52 SS.reconfigure :exhale @15:18:11.56 SS.hold @15:18:18.91 Intake.select :close @15:18:19.05 SSV.dial :exhaust @15:18:19.79 Exhaust.select :open @15:18:19.90 SS.seek 0ml @15:18:28.66 Exhaust.select :close @15:18:28.81 SSV.dial CBV @15:18:28.84
CBV.dial SSV @15:18:29.60 Allowing 130s for 25.0ml of remaining 925.0ml @15:18:29.61 Intake.select :open @15:18:31.77 Intake @16.0psia, vacuum from 10.0 to 13.0psi @15:18:31.78 SS.reconfigure :inhaling 1.61ml/s @15:18:31.83 SS.seek 25ml @15:18:42.86 Vacuum too high -- waiting up to 40 seconds @15:18:42.87 SS.reconfigure :exhale @15:18:42.91 SS.hold @15:18:43.71 Intake @13.5psia, vacuum from 8.0 to 11.0psi @15:18:43.73 SS.reconfigure :inhaling 1.45ml/s @15:18:43.77 SS.seek 25ml @15:18:52.90 Stroke completed -- waiting for pressure to equalize ... @15:18:52.91 SS.reconfigure :exhale @15:18:52.95 SS.hold @15:19:01.42 Intake.select :close @15:19:02.03 SSV.dial :exhaust @15:19:02.79 Exhaust.select :open @15:19:02.90 SS.seek 0ml @15:19:11.94 Exhaust.select :close @15:19:12.05 SSV.dial CBV @15:19:12.08
CBV.dial SSV @15:19:12.84 Allowing 130s for 25.0ml of remaining 900.0ml @15:19:12.85 Intake.select :open @15:19:15.00 Intake @15.9psia, vacuum from 10.0 to 13.0psi @15:19:15.02 SS.reconfigure :inhaling 1.45ml/s @15:19:15.06 SS.seek 25ml @15:19:29.86 Vacuum too high -- waiting up to 40 seconds @15:19:29.87 SS.reconfigure :exhale @15:19:29.91 SS.hold @15:19:33.03 Intake @14.2psia, vacuum from 8.7 to 11.7psi @15:19:33.04 SS.reconfigure :inhaling 1.30ml/s @15:19:33.08 SS.seek 25ml @15:19:39.51 Stroke completed -- waiting for pressure to equalize ... @15:19:39.52 SS.reconfigure :exhale @15:19:39.56 SS.hold @15:19:45.13 Intake.select :close @15:19:45.26 SSV.dial :exhaust @15:19:46.02 Exhaust.select :open @15:19:46.13 SS.seek 0ml @15:19:55.10 Exhaust.select :close @15:19:55.20 SSV.dial CBV @15:19:55.22
CBV.dial SSV @15:19:55.99 Allowing 130s for 25.0ml of remaining 875.0ml @15:19:56.00 Intake.select :open @15:19:58.16 Intake @15.9psia, vacuum from 10.0 to 13.0psi @15:19:58.17 SS.reconfigure :inhaling 1.30ml/s @15:19:58.21 SS.seek 25ml @15:20:16.48 Vacuum too high -- waiting up to 40 seconds @15:20:16.49 SS.reconfigure :exhale @15:20:16.53 SS.hold @15:20:20.11 Intake @14.7psia, vacuum from 9.2 to 12.2psi @15:20:20.13 SS.reconfigure :inhaling 1.17ml/s @15:20:20.17 SS.seek 25ml @15:20:24.05 Stroke completed -- waiting for pressure to equalize ... @15:20:24.06 SS.reconfigure :exhale @15:20:24.10 SS.hold @15:20:28.87 Intake.select :close @15:20:29.00 SSV.dial :exhaust @15:20:29.76 Exhaust.select :open @15:20:29.86 SS.seek 0ml @15:20:38.66 Exhaust.select :close @15:20:38.76 SSV.dial CBV @15:20:38.78
CBV.dial SSV @15:20:39.56 Allowing 130s for 25.0ml of remaining 850.0ml @15:20:39.57 Intake.select :open @15:20:41.72 Intake @16.0psia, vacuum from 10.0 to 13.0psi @15:20:41.73 SS.reconfigure :inhaling 1.17ml/s @15:20:41.78 SS.seek 25ml @15:21:05.86 Stroke completed -- waiting for pressure to equalize ... @15:21:05.87 SS.reconfigure :exhale @15:21:05.91 SS.hold @15:21:14.39 Intake.select :close @15:21:14.53 SSV.dial :exhaust @15:21:15.29 Exhaust.select :open @15:21:15.39 SS.seek 0ml @15:21:24.16 Exhaust.select :close @15:21:24.26 SSV.dial CBV @15:21:24.28
CBV.dial SSV @15:21:25.05 Allowing 130s for 25.0ml of remaining 825.0ml @15:21:25.06 Intake.select :open @15:21:27.21 Intake @16.0psia, vacuum from 10.0 to 13.0psi @15:21:27.23 SS.reconfigure :inhaling 1.17ml/s @15:21:27.27 SS.seek 25ml @15:21:47.92 Vacuum too high -- waiting up to 40 seconds @15:21:47.93 SS.reconfigure :exhale @15:21:47.97 SS.hold @15:21:51.54 Intake @14.6psia, vacuum from 9.1 to 12.1psi @15:21:51.56 SS.reconfigure :inhaling 1.06ml/s @15:21:51.60 SS.seek 25ml @15:21:55.66 Stroke completed -- waiting for pressure to equalize ... @15:21:55.67 SS.reconfigure :exhale @15:21:55.71 SS.hold @15:22:01.00 Intake.select :close @15:22:01.14 SSV.dial :exhaust @15:22:01.90 Exhaust.select :open @15:22:02.00 SS.seek 0ml @15:22:10.96 Exhaust.select :close @15:22:11.06 SSV.dial CBV @15:22:11.08
CBV.dial SSV @15:22:11.85 Allowing 130s for 25.0ml of remaining 800.0ml @15:22:11.86 Intake.select :open @15:22:14.01 Intake @16.0psia, vacuum from 10.0 to 13.0psi @15:22:14.03 SS.reconfigure :inhaling 1.06ml/s @15:22:14.07 SS.seek 25ml @15:22:40.03 Stroke completed -- waiting for pressure to equalize ... @15:22:40.04 SS.reconfigure :exhale @15:22:40.08 SS.hold @15:22:48.58 Intake.select :close @15:22:48.71 SSV.dial :exhaust @15:22:49.48 Exhaust.select :open @15:22:49.58 SS.seek 0ml @15:22:58.36 Exhaust.select :close @15:22:58.47 SSV.dial CBV @15:22:58.50
CBV.dial SSV @15:22:59.24 Allowing 130s for 25.0ml of remaining 775.0ml @15:22:59.25 Intake.select :open @15:23:01.40 Intake @16.1psia, vacuum from 10.0 to 13.0psi @15:23:01.42 SS.reconfigure :inhaling 1.06ml/s @15:23:01.46 SS.seek 25ml @15:23:25.29 Vacuum too high -- waiting up to 40 seconds @15:23:25.30 SS.reconfigure :exhale @15:23:25.34 SS.hold @15:23:29.50 Intake @15.2psia, vacuum from 9.7 to 12.7psi @15:23:29.51 SS.reconfigure :inhaling 0.95ml/s @15:23:29.55 SS.seek 25ml @15:23:32.56 Stroke completed -- waiting for pressure to equalize ... @15:23:32.57 SS.reconfigure :exhale @15:23:32.61 SS.hold @15:23:37.38 Intake.select :close @15:23:37.52 SSV.dial :exhaust @15:23:38.27 Exhaust.select :open @15:23:38.37 SS.seek 0ml @15:23:47.13 Exhaust.select :close @15:23:47.23 SSV.dial CBV @15:23:47.25
CBV.dial SSV @15:23:48.02 Allowing 130s for 25.0ml of remaining 750.0ml @15:23:48.03 Intake.select :open @15:23:50.18 Intake @16.1psia, vacuum from 10.0 to 13.0psi @15:23:50.20 SS.reconfigure :inhaling 0.95ml/s @15:23:50.24 SS.seek 25ml @15:24:19.11 Stroke completed -- waiting for pressure to equalize ... @15:24:19.12 SS.reconfigure :exhale @15:24:19.16 SS.hold @15:24:27.59 Intake.select :close @15:24:27.72 SSV.dial :exhaust @15:24:28.47 Exhaust.select :open @15:24:28.58 SS.seek 0ml @15:24:37.30 Exhaust.select :close @15:24:37.40 SSV.dial CBV @15:24:37.42
CBV.dial SSV @15:24:38.19 Allowing 130s for 25.0ml of remaining 725.0ml @15:24:38.20 Intake.select :open @15:24:40.35 Intake @16.1psia, vacuum from 10.0 to 13.0psi @15:24:40.37 SS.reconfigure :inhaling 0.95ml/s @15:24:40.41 SS.seek 25ml @15:25:09.30 Stroke completed -- waiting for pressure to equalize ... @15:25:09.31 SS.reconfigure :exhale @15:25:09.35 SS.hold @15:25:18.26 Intake.select :close @15:25:18.40 SSV.dial :exhaust @15:25:19.16 Exhaust.select :open @15:25:19.26 SS.seek 0ml @15:25:27.97 Exhaust.select :close @15:25:28.07 SSV.dial CBV @15:25:28.09
CBV.dial SSV @15:25:28.86 Allowing 130s for 25.0ml of remaining 700.0ml @15:25:28.87 Intake.select :open @15:25:31.03 Intake @16.1psia, vacuum from 10.0 to 13.0psi @15:25:31.04 SS.reconfigure :inhaling 0.95ml/s @15:25:31.08 SS.seek 25ml @15:25:56.26 Vacuum too high -- waiting up to 40 seconds @15:25:56.27 SS.reconfigure :exhale @15:25:56.31 SS.hold @15:26:00.46 Intake @15.1psia, vacuum from 9.6 to 12.6psi @15:26:00.48 SS.reconfigure :inhaling 0.87ml/s @15:26:00.52 SS.seek 25ml @15:26:04.81 Stroke completed -- waiting for pressure to equalize ... @15:26:04.82 SS.reconfigure :exhale @15:26:04.86 SS.hold @15:26:10.41 Intake.select :close @15:26:10.54 SSV.dial :exhaust @15:26:11.30 Exhaust.select :open @15:26:11.40 SS.seek 0ml @15:26:20.19 Exhaust.select :close @15:26:20.29 SSV.dial CBV @15:26:20.31
CBV.dial SSV @15:26:21.08 Allowing 130s for 25.0ml of remaining 675.0ml @15:26:21.09 Intake.select :open @15:26:23.24 Intake @16.1psia, vacuum from 10.0 to 13.0psi @15:26:23.26 SS.reconfigure :inhaling 0.87ml/s @15:26:23.30 SS.seek 25ml @15:26:54.84 Stroke completed -- waiting for pressure to equalize ... @15:26:54.85 SS.reconfigure :exhale @15:26:54.89 SS.hold @15:27:04.55 Intake.select :close @15:27:04.69 SSV.dial :exhaust @15:27:05.45 Exhaust.select :open @15:27:05.56 SS.seek 0ml @15:27:14.31 Exhaust.select :close @15:27:14.42 SSV.dial CBV @15:27:14.43
CBV.dial SSV @15:27:15.20 Allowing 130s for 25.0ml of remaining 650.0ml @15:27:15.21 Intake.select :open @15:27:17.36 Intake @16.0psia, vacuum from 10.0 to 13.0psi @15:27:17.38 SS.reconfigure :inhaling 0.87ml/s @15:27:17.42 SS.seek 25ml @15:27:48.73 Stroke completed -- waiting for pressure to equalize ... @15:27:48.74 SS.reconfigure :exhale @15:27:48.78 SS.hold @15:27:58.42 Intake.select :close @15:27:58.56 SSV.dial :exhaust @15:27:59.32 Exhaust.select :open @15:27:59.42 SS.seek 0ml @15:28:08.26 Exhaust.select :close @15:28:08.37 SSV.dial CBV @15:28:08.38
CBV.dial SSV @15:28:09.17 Allowing 130s for 25.0ml of remaining 625.0ml @15:28:09.18 Intake.select :open @15:28:11.33 Intake @16.1psia, vacuum from 10.0 to 13.0psi @15:28:11.35 SS.reconfigure :inhaling 0.87ml/s @15:28:11.39 SS.seek 25ml @15:28:37.38 Vacuum too high -- waiting up to 40 seconds @15:28:37.39 SS.reconfigure :exhale @15:28:37.43 SS.hold @15:28:41.58 Intake @15.0psia, vacuum from 9.5 to 12.5psi @15:28:41.59 SS.reconfigure :inhaling 0.78ml/s @15:28:41.64 SS.seek 25ml @15:28:48.12 Stroke completed -- waiting for pressure to equalize ... @15:28:48.52 SS.reconfigure :exhale @15:28:48.65 SS.hold @15:28:55.00 Intake.select :close @15:28:55.13 SSV.dial :exhaust @15:28:55.89 Exhaust.select :open @15:28:56.00 SS.seek 0ml @15:29:04.83 Exhaust.select :close @15:29:04.93 SSV.dial CBV @15:29:04.95
CBV.dial SSV @15:29:05.72 Allowing 130s for 25.0ml of remaining 600.0ml @15:29:05.73 Intake.select :open @15:29:07.88 Intake @16.1psia, vacuum from 10.0 to 13.0psi @15:29:07.89 SS.reconfigure :inhaling 0.78ml/s @15:29:07.94 SS.seek 25ml @15:29:42.75 Stroke completed -- waiting for pressure to equalize ... @15:29:42.76 SS.reconfigure :exhale @15:29:42.80 SS.hold @15:29:52.44 Intake.select :close @15:29:52.58 SSV.dial :exhaust @15:29:53.34 Exhaust.select :open @15:29:53.45 SS.seek 0ml @15:30:02.43 Exhaust.select :close @15:30:02.54 SSV.dial CBV @15:30:02.57
CBV.dial SSV @15:30:03.33 Allowing 130s for 25.0ml of remaining 575.0ml @15:30:03.34 Intake.select :open @15:30:05.49 Intake @16.1psia, vacuum from 10.0 to 13.0psi @15:30:05.50 SS.reconfigure :inhaling 0.78ml/s @15:30:05.55 SS.seek 25ml @15:30:35.04 Vacuum too high -- waiting up to 40 seconds SS.reconfigure :exhale @15:30:35.08 SS.hold @15:30:39.23 Intake @15.0psia, vacuum from 9.5 to 12.5psi @15:30:39.25 SS.reconfigure :inhaling 0.70ml/s @15:30:39.29 SS.seek 25ml @15:30:44.76 Stroke completed -- waiting for pressure to equalize ... @15:30:44.77 SS.reconfigure :exhale @15:30:44.81 SS.hold @15:30:53.25 Intake.select :close @15:30:53.38 SSV.dial :exhaust @15:30:54.14 Exhaust.select :open @15:30:54.24 SS.seek 0ml @15:31:02.98 Exhaust.select :close @15:31:03.08 SSV.dial CBV @15:31:03.10
CBV.dial SSV @15:31:03.87 Allowing 130s for 25.0ml of remaining 550.0ml @15:31:03.88 Intake.select :open @15:31:06.03 Intake @16.0psia, vacuum from 10.0 to 13.0psi @15:31:06.05 SS.reconfigure :inhaling 0.70ml/s @15:31:06.09 SS.seek 25ml @15:31:44.04 Stroke completed -- waiting for pressure to equalize ... @15:31:44.05 SS.reconfigure :exhale @15:31:44.09 SS.hold @15:31:55.20 Intake.select :close @15:31:55.34 SSV.dial :exhaust @15:31:56.09 Exhaust.select :open @15:31:56.20 SS.seek 0ml @15:32:05.06 Exhaust.select :close @15:32:05.16 SSV.dial CBV @15:32:05.18
CBV.dial SSV @15:32:05.95 Allowing 130s for 25.0ml of remaining 525.0ml @15:32:05.96 Intake.select :open @15:32:08.11 Intake @16.1psia, vacuum from 10.0 to 13.0psi @15:32:08.13 SS.reconfigure :inhaling 0.70ml/s @15:32:08.17 SS.seek 25ml @15:32:43.97 Vacuum too high -- waiting up to 40 seconds @15:32:43.98 SS.reconfigure :exhale @15:32:44.02 SS.hold @15:32:49.64 Intake @15.8psia, vacuum from 10.0 to 13.0psi @15:32:49.66 SS.reconfigure :inhaling 0.63ml/s @15:32:49.70 SS.seek 25ml @15:32:52.56 Stroke completed -- waiting for pressure to equalize ... @15:32:52.57 SS.reconfigure :exhale @15:32:52.61 SS.hold @15:32:59.08 Intake.select :close @15:32:59.22 SSV.dial :exhaust @15:32:59.98 Exhaust.select :open @15:33:00.08 SS.seek 0ml @15:33:09.05 Exhaust.select :close @15:33:09.16 SSV.dial CBV @15:33:09.18
CBV.dial SSV @15:33:09.95 Allowing 130s for 25.0ml of remaining 500.0ml @15:33:09.96 Intake.select :open @15:33:12.11 Intake @16.1psia, vacuum from 10.0 to 13.0psi @15:33:12.12 SS.reconfigure :inhaling 0.63ml/s @15:33:12.17 SS.seek 25ml @15:33:53.88 Stroke completed -- waiting for pressure to equalize ... @15:33:53.89 SS.reconfigure :exhale @15:33:53.93 SS.hold @15:34:04.98 Intake.select :close @15:34:05.12 SSV.dial :exhaust @15:34:05.89 Exhaust.select :open @15:34:05.99 SS.seek 0ml @15:34:14.71 Exhaust.select :close @15:34:14.81 SSV.dial CBV @15:34:14.83
CBV.dial SSV @15:34:15.60 Allowing 130s for 25.0ml of remaining 475.0ml @15:34:15.61 Intake.select :open @15:34:17.76 Intake @16.1psia, vacuum from 10.0 to 13.0psi @15:34:17.78 SS.reconfigure :inhaling 0.63ml/s @15:34:17.82 SS.seek 25ml @15:34:59.52 Stroke completed -- waiting for pressure to equalize ... @15:34:59.53 SS.reconfigure :exhale @15:34:59.57 SS.hold @15:35:12.10 Intake.select :close @15:35:12.23 SSV.dial :exhaust @15:35:12.99 Exhaust.select :open @15:35:13.61 SS.seek 0ml @15:35:22.37 Exhaust.select :close @15:35:22.47 SSV.dial CBV @15:35:22.49
CBV.dial SSV @15:35:23.27 Allowing 130s for 25.0ml of remaining 450.0ml @15:35:23.28 Intake.select :open @15:35:25.44 Intake @16.1psia, vacuum from 10.0 to 13.0psi @15:35:25.45 SS.reconfigure :inhaling 0.63ml/s @15:35:25.49 SS.seek 25ml @15:36:07.52 Stroke completed -- waiting for pressure to equalize ... @15:36:07.53 SS.reconfigure :exhale @15:36:07.57 SS.hold @15:36:20.11 Intake.select :close @15:36:20.25 SSV.dial :exhaust @15:36:21.01 Exhaust.select :open @15:36:21.11 SS.seek 0ml @15:36:29.99 Exhaust.select :close @15:36:30.09 SSV.dial CBV @15:36:30.11
CBV.dial SSV @15:36:30.88 Allowing 130s for 25.0ml of remaining 425.0ml @15:36:30.89 Intake.select :open @15:36:33.04 Intake @16.0psia, vacuum from 10.0 to 13.0psi @15:36:33.06 SS.reconfigure :inhaling 0.63ml/s @15:36:33.10 SS.seek 25ml @15:37:15.38 Stroke completed -- waiting for pressure to equalize ... @15:37:15.39 SS.reconfigure :exhale @15:37:15.43 SS.hold @15:37:29.66 Intake.select :close @15:37:29.80 SSV.dial :exhaust @15:37:30.55 Exhaust.select :open @15:37:30.66 SS.seek 0ml @15:37:39.50 Exhaust.select :close @15:37:39.61 SSV.dial CBV @15:37:39.66
CBV.dial SSV @15:37:40.38 Allowing 130s for 25.0ml of remaining 400.0ml @15:37:40.39 Intake.select :open @15:37:42.54 Intake @16.1psia, vacuum from 10.0 to 13.0psi @15:37:42.56 SS.reconfigure :inhaling 0.63ml/s @15:37:42.60 SS.seek 25ml @15:38:15.18 Vacuum too high -- waiting up to 40 seconds @15:38:15.19 SS.reconfigure :exhale @15:38:15.23 SS.hold @15:38:22.67 Intake @16.2psia, vacuum from 10.0 to 13.0psi @15:38:22.68 SS.reconfigure :inhaling 0.57ml/s @15:38:22.73 SS.seek 25ml @15:38:33.77 Stroke completed -- waiting for pressure to equalize ... @15:38:33.78 SS.reconfigure :exhale @15:38:33.82 SS.hold @15:38:44.85 Intake.select :close @15:38:44.98 SSV.dial :exhaust @15:38:45.74 Exhaust.select :open @15:38:46.31 SS.seek 0ml @15:38:55.14 Exhaust.select :close @15:38:55.25 SSV.dial CBV @15:38:55.30
CBV.dial SSV @15:38:56.02 Allowing 130s for 25.0ml of remaining 375.0ml @15:38:56.03 Intake.select :open @15:38:58.19 Intake @16.1psia, vacuum from 10.0 to 13.0psi @15:38:58.21 SS.reconfigure :inhaling 0.57ml/s @15:38:58.25 SS.seek 25ml @15:39:44.41 Stroke completed -- waiting for pressure to equalize ... @15:39:44.42 SS.reconfigure :exhale @15:39:44.46 SS.hold @15:40:00.63 Intake.select :close @15:40:00.76 SSV.dial :exhaust @15:40:01.50 Exhaust.select :open @15:40:01.61 SS.seek 0ml @15:40:10.66 Exhaust.select :close @15:40:10.76 SSV.dial CBV @15:40:10.78
CBV.dial SSV @15:40:11.55 Allowing 130s for 25.0ml of remaining 350.0ml @15:40:11.56 Intake.select :open @15:40:13.71 Intake @16.2psia, vacuum from 10.0 to 13.0psi @15:40:13.73 SS.reconfigure :inhaling 0.57ml/s @15:40:13.77 SS.seek 25ml @15:40:49.43 Vacuum too high -- waiting up to 40 seconds @15:40:49.44 SS.reconfigure :exhale @15:40:49.48 SS.hold @15:40:57.91 Intake @16.7psia, vacuum from 10.0 to 13.0psi @15:40:57.92 SS.reconfigure :inhaling 0.51ml/s @15:40:57.97 SS.seek 25ml @15:41:09.33 Stroke completed -- waiting for pressure to equalize ... @15:41:09.34 SS.reconfigure :exhale @15:41:09.38 SS.hold @15:41:20.38 Intake.select :close @15:41:20.51 SSV.dial :exhaust @15:41:21.27 Exhaust.select :open @15:41:21.37 SS.seek 0ml @15:41:30.11 Exhaust.select :close @15:41:30.21 SSV.dial CBV @15:41:30.28
CBV.dial SSV @15:41:31.05 Allowing 130s for 25.0ml of remaining 325.0ml @15:41:31.06 Intake.select :open @15:41:33.21 Intake @16.2psia, vacuum from 10.0 to 13.0psi @15:41:33.22 SS.reconfigure :inhaling 0.51ml/s @15:41:33.27 SS.seek 25ml @15:42:24.26 Stroke completed -- waiting for pressure to equalize ... @15:42:24.27 SS.reconfigure :exhale @15:42:24.31 SS.hold @15:42:40.94 Intake.select :close @15:42:41.07 SSV.dial :exhaust @15:42:41.83 Exhaust.select :open @15:42:41.93 SS.seek 0ml @15:42:50.95 Exhaust.select :close @15:42:51.05 SSV.dial CBV @15:42:51.07
CBV.dial SSV @15:42:51.84 Allowing 130s for 25.0ml of remaining 300.0ml @15:42:51.85 Intake.select :open @15:42:54.01 Intake @16.3psia, vacuum from 10.0 to 13.0psi @15:42:54.02 SS.reconfigure :inhaling 0.51ml/s @15:42:54.06 SS.seek 25ml @15:43:39.08 Vacuum too high -- waiting up to 40 seconds @15:43:39.09 SS.reconfigure :exhale @15:43:39.13 SS.hold @15:43:46.47 Intake @16.0psia, vacuum from 10.0 to 13.0psi @15:43:46.49 SS.reconfigure :inhaling 0.47ml/s @15:43:46.53 SS.seek 25ml @15:43:53.36 Stroke completed -- waiting for pressure to equalize ... @15:43:53.37 SS.reconfigure :exhale @15:43:53.41 SS.hold @15:44:04.51 Intake.select :close @15:44:04.65 SSV.dial :exhaust @15:44:05.40 Exhaust.select :open @15:44:05.51 SS.seek 0ml @15:44:14.54 Exhaust.select :close @15:44:14.65 SSV.dial CBV @15:44:14.70
CBV.dial SSV @15:44:15.42 Allowing 130s for 25.0ml of remaining 275.0ml @15:44:15.43 Intake.select :open @15:44:17.58 Intake @16.2psia, vacuum from 10.0 to 13.0psi @15:44:17.60 SS.reconfigure :inhaling 0.47ml/s @15:44:17.64 SS.seek 25ml @15:45:13.32 Stroke completed -- waiting for pressure to equalize ... @15:45:13.33 SS.reconfigure :exhale @15:45:13.37 SS.hold @15:45:29.54 Intake.select :close @15:45:29.68 SSV.dial :exhaust @15:45:30.43 Exhaust.select :open @15:45:30.54 SS.seek 0ml @15:45:39.57 Exhaust.select :close @15:45:39.67 SSV.dial CBV @15:45:39.70
CBV.dial SSV @15:45:40.47 Allowing 130s for 25.0ml of remaining 250.0ml Intake.select :open @15:45:42.62 Intake @16.1psia, vacuum from 10.0 to 13.0psi @15:45:42.64 SS.reconfigure :inhaling 0.47ml/s @15:45:42.68 SS.seek 25ml @15:46:38.15 Stroke completed -- waiting for pressure to equalize ... @15:46:38.16 SS.reconfigure :exhale @15:46:38.20 SS.hold @15:46:56.90 Intake.select :close @15:46:57.03 SSV.dial :exhaust @15:46:57.79 Exhaust.select :open @15:46:57.90 SS.seek 0ml @15:47:06.96 Exhaust.select :close @15:47:07.06 SSV.dial CBV @15:47:07.09
CBV.dial SSV @15:47:07.85 Allowing 130s for 25.0ml of remaining 225.0ml @15:47:07.86 Intake.select :open @15:47:10.02 Intake @16.1psia, vacuum from 10.0 to 13.0psi @15:47:10.04 SS.reconfigure :inhaling 0.47ml/s @15:47:10.08 SS.seek 25ml @15:48:05.68 Stroke completed -- waiting for pressure to equalize ... @15:48:05.69 SS.reconfigure :exhale @15:48:05.73 SS.hold @15:48:25.98 Intake.select :close @15:48:26.12 SSV.dial :exhaust @15:48:26.87 Exhaust.select :open @15:48:26.98 SS.seek 0ml @15:48:35.77 Exhaust.select :close @15:48:35.87 SSV.dial CBV @15:48:35.90
CBV.dial SSV @15:48:36.67 Allowing 130s for 25.0ml of remaining 200.0ml Intake.select :open @15:48:38.82 Intake @16.2psia, vacuum from 10.0 to 13.0psi @15:48:38.84 SS.reconfigure :inhaling 0.47ml/s @15:48:38.88 SS.seek 25ml @15:49:18.42 Vacuum too high -- waiting up to 40 seconds @15:49:18.43 SS.reconfigure :exhale @15:49:18.47 SS.hold @15:49:26.92 Intake @16.3psia, vacuum from 10.0 to 13.0psi @15:49:26.93 SS.reconfigure :inhaling 0.43ml/s @15:49:26.98 SS.seek 25ml @15:49:45.79 Stroke completed -- waiting for pressure to equalize ... @15:49:45.80 SS.reconfigure :exhale @15:49:45.84 SS.hold @15:50:02.01 Intake.select :close @15:50:02.15 SSV.dial :exhaust @15:50:02.91 Exhaust.select :open @15:50:03.02 SS.seek 0ml @15:50:12.07 Exhaust.select :close @15:50:12.17 SSV.dial CBV @15:50:12.19
CBV.dial SSV @15:50:12.96 Allowing 130s for 25.0ml of remaining 175.0ml @15:50:12.97 Intake.select :open @15:50:15.12 Intake @16.3psia, vacuum from 10.0 to 13.0psi @15:50:15.14 SS.reconfigure :inhaling 0.43ml/s @15:50:15.18 SS.seek 25ml @15:51:14.67 Vacuum too high -- waiting up to 40 seconds @15:51:14.68 SS.reconfigure :exhale @15:51:14.72 SS.hold @15:51:24.58 Intake @16.5psia, vacuum from 10.0 to 13.0psi @15:51:24.60 SS.reconfigure :inhaling 0.38ml/s @15:51:24.64 SS.seek 25ml @15:51:27.50 Stroke completed -- waiting for pressure to equalize ... @15:51:27.51 SS.reconfigure :exhale @15:51:27.56 SS.hold @15:51:40.18 Intake.select :close @15:51:40.32 SSV.dial :exhaust @15:51:41.29 Exhaust.select :open @15:51:41.40 SS.seek 0ml @15:51:50.42 Exhaust.select :close @15:51:50.53 SSV.dial CBV @15:51:50.55
CBV.dial SSV @15:51:51.32 Allowing 130s for 25.0ml of remaining 150.0ml @15:51:51.33 Intake.select :open @15:51:53.48 Intake @16.5psia, vacuum from 10.0 to 13.0psi @15:51:53.49 SS.reconfigure :inhaling 0.38ml/s @15:51:53.55 SS.seek 25ml @15:53:04.14 Stroke completed -- waiting for pressure to equalize ... @15:53:04.15 SS.reconfigure :exhale @15:53:04.19 SS.hold @15:53:24.44 Intake.select :close @15:53:24.58 SSV.dial :exhaust @15:53:25.35 Exhaust.select :open @15:53:25.46 SS.seek 0ml @15:53:34.56 Exhaust.select :close @15:53:34.67 SSV.dial CBV @15:53:34.72
CBV.dial SSV @15:53:35.44 Allowing 130s for 25.0ml of remaining 125.0ml @15:53:35.45 Intake.select :open @15:53:37.60 Intake @16.4psia, vacuum from 10.0 to 13.0psi @15:53:37.62 SS.reconfigure :inhaling 0.38ml/s @15:53:37.66 SS.seek 25ml @15:54:48.76 Stroke completed -- waiting for pressure to equalize ... @15:54:48.77 SS.reconfigure :exhale @15:54:48.81 SS.hold @15:55:11.10 Intake.select :close @15:55:11.23 SSV.dial :exhaust @15:55:11.98 Exhaust.select :open @15:55:12.09 SS.seek 0ml @15:55:21.15 Exhaust.select :close @15:55:21.26 SSV.dial CBV @15:55:21.31
CBV.dial SSV @15:55:22.03 Allowing 130s for 25.0ml of remaining 100.0ml @15:55:22.04 Intake.select :open @15:55:24.20 Intake @16.4psia, vacuum from 10.0 to 13.0psi @15:55:24.21 SS.reconfigure :inhaling 0.38ml/s @15:55:24.26 SS.seek 25ml @15:56:36.84 Stroke completed -- waiting for pressure to equalize ... @15:56:36.85 SS.reconfigure :exhale @15:56:36.89 SS.hold @15:57:01.32 Intake.select :close @15:57:01.45 SSV.dial :exhaust @15:57:02.22 Exhaust.select :open @15:57:02.32 SS.seek 0ml @15:57:11.39 Exhaust.select :close @15:57:11.49 SSV.dial CBV @15:57:11.51
CBV.dial SSV @15:57:12.29 Allowing 130s for 25.0ml of remaining 75.0ml @15:57:12.30 Intake.select :open @15:57:14.45 Intake @16.5psia, vacuum from 10.0 to 13.0psi @15:57:14.46 SS.reconfigure :inhaling 0.38ml/s @15:57:14.51 SS.seek 25ml @15:58:09.06 Vacuum too high -- waiting up to 40 seconds @15:58:09.07 SS.reconfigure :exhale @15:58:09.12 SS.hold @15:58:18.89 Intake @16.3psia, vacuum from 10.0 to 13.0psi @15:58:18.90 SS.reconfigure :inhaling 0.34ml/s @15:58:18.95 SS.seek 25ml @15:58:38.87 Stroke completed -- waiting for pressure to equalize ... @15:58:38.88 SS.reconfigure :exhale @15:58:38.92 SS.hold @15:58:57.12 Intake.select :close @15:58:57.26 SSV.dial :exhaust @15:58:58.01 Exhaust.select :open @15:58:58.12 SS.seek 0ml @15:59:07.21 Exhaust.select :close @15:59:07.31 SSV.dial CBV @15:59:07.33
CBV.dial SSV @15:59:08.10 Allowing 130s for 25.0ml of remaining 50.0ml @15:59:08.11 Intake.select :open @15:59:10.26 Intake @16.4psia, vacuum from 10.0 to 13.0psi @15:59:10.28 SS.reconfigure :inhaling 0.34ml/s @15:59:10.32 SS.seek 25ml @16:00:36.55 Stroke completed -- waiting for pressure to equalize ... @16:00:36.56 SS.reconfigure :exhale @16:00:36.60 SS.hold @16:00:58.90 Intake.select :close @16:00:59.03 SSV.dial :exhaust @16:00:59.79 Exhaust.select :open @16:00:59.90 SS.seek 0ml @16:01:08.96 Exhaust.select :close @16:01:09.07 SSV.dial CBV @16:01:09.08
CBV.dial SSV @16:01:09.86 Allowing 130s for 25.0ml of remaining 25.0ml @16:01:09.87 Intake.select :open @16:01:12.02 Intake @16.4psia, vacuum from 10.0 to 13.0psi @16:01:12.04 SS.reconfigure :inhaling 0.34ml/s @16:01:12.08 SS.seek 24.96ml @16:02:36.04 Stroke completed -- waiting for pressure to equalize ... @16:02:36.05 SS.reconfigure :exhale @16:02:36.09 SS.hold @16:03:00.51 Intake.select :close @16:03:00.64 SSV.dial :exhaust @16:03:01.40 Exhaust.select :open @16:03:01.50 SS.seek 0ml @16:03:10.36 Exhaust.select :close @16:03:10.47 Sampled 1000.0ml @16:03:10.48 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @16:03:10.49 Intake.select :close @16:03:10.54 CSV.dial :waste @16:03:10.59 CS.seek 0ml @16:03:10.68 SSV.dial CBV,avoiding: :exhaust @16:03:10.70
CBV.dial SSV @16:03:10.76 CSV.dial :air @16:03:11.50 CS.seek 10ml @16:03:12.59
CTV.dial :air @16:03:13.38 delay 15 seconds @16:03:28.40 SSV.dial :spit,avoiding: :exhaust @16:03:29.73 CTV.dial CSV,avoiding: :intake @16:03:29.76
SS.seek 0ml @16:03:29.88 SSV.dial CBV,avoiding: :exhaust @16:03:36.25 CBV.dial between SSV and waste2 @16:03:36.62 CSV.dial between air and CTV @16:03:36.92 CS.seek 8.427ml @16:03:41.36 CTV.dial CSV,avoiding: :air @16:03:41.38
CSV.dial CTV @16:03:41.70 CS.reconfigure :pressurize @16:03:41.74 CS.seek 0ml @16:03:58.03 BubblePoint: Intake @39.3psia, Outlet @15.2psia @16:03:58.04 CBV.dial SSV @16:03:58.38 SS.reconfigure :default @16:03:58.42 SS.seek 10ml @16:04:04.02 delay 15 seconds @16:04:19.13 Evacuated: Intake @17.0psia, Outlet @9.3psia @16:04:19.14 SSV.dial :spit,avoiding: :exhaust @16:04:20.48 SS.reconfigure :exhale @16:04:20.52 SS.seek 0ml @16:04:25.82 SS.reconfigure :fast @16:04:25.86 SS.seek 5ml @16:04:28.71 SSV.dial CBV,avoiding: :exhaust @16:04:28.73
CBV.dial SSV @16:04:30.05 CTV.dial :air,avoiding: :intake @16:04:31.43 CSV.dial CTV @16:04:31.46
SS.seek 10ml @16:04:32.45 CSV.dial :air @16:04:32.91 CS.reconfigure :fast @16:04:32.96 CS.seek 0ml @16:04:33.94
SSV.dial :spit,avoiding: :exhaust @16:04:35.28 SS.reconfigure :exhale @16:04:35.32 SS.seek 0ml @16:04:39.00 CS.stop @16:04:40.68
SS.reconfigure :fast @16:04:40.72 SS.seek 5ml @16:04:43.82 SSV.dial CBV,avoiding: :exhaust @16:04:43.84
CBV.dial SSV @16:04:46.17 SSV.dial :spit,avoiding: :exhaust @16:04:47.48 SS.reconfigure :default @16:04:47.96 SS.seek 0ml @16:04:51.14 SS.stop @16:04:51.18 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @16:04:51.19 Intake.select :close @16:04:51.26 CSV.dial :waste @16:04:51.38 SSV.dial CBV,avoiding: :exhaust @16:04:51.39
CBV.dial SSV @16:04:51.99 CS.seek 0ml @16:04:52.06 CSV.dial :air @16:04:52.73
CTV.dial :air @16:04:52.74 delay 15 seconds @16:04:52.79 CS.seek 10ml @16:05:07.75
SSV.dial :spit,avoiding: :exhaust @16:05:09.07 CTV.dial CSV,avoiding: :intake @16:05:09.10
SS.reconfigure :exhale @16:05:09.20 SS.seek 0ml @16:05:09.61 SSV.dial CBV,avoiding: :exhaust @16:05:17.46 CBV.dial between SSV and waste2 @16:05:17.86 CTV.dial CSV,avoiding: :air @16:05:17.88
CSV.dial CTV @16:05:18.32 CS.reconfigure :pressurize @16:05:18.36 CS.seek 0ml @16:05:39.41 BubblePoint: Intake @38.3psia, Outlet @14.1psia CBV.dial SSV @16:05:39.74 SS.reconfigure :default @16:05:39.78 SS.seek 10ml @16:05:45.38 delay 15 seconds @16:06:00.49 Evacuated: Intake @19.0psia, Outlet @11.6psia @16:06:00.50 SSV.dial :spit,avoiding: :exhaust @16:06:01.84 SS.reconfigure :exhale @16:06:01.88 SS.seek 0ml @16:06:07.51 SS.reconfigure :fast @16:06:07.55 SS.seek 5ml @16:06:10.46 SSV.dial CBV,avoiding: :exhaust @16:06:10.48
CBV.dial SSV @16:06:11.80 CTV.dial :air,avoiding: :intake @16:06:13.16 CSV.dial CTV @16:06:13.20
SS.seek 10ml @16:06:14.19 CSV.dial :air @16:06:14.65 CS.reconfigure :fast @16:06:14.69 CS.seek 0ml @16:06:15.86
SSV.dial :spit,avoiding: :exhaust @16:06:17.18 SS.reconfigure :exhale @16:06:17.22 SS.seek 0ml @16:06:20.12 CS.stop @16:06:22.57
SS.reconfigure :fast @16:06:22.61 SS.seek 5ml @16:06:25.46 SSV.dial CBV,avoiding: :exhaust @16:06:25.48
CBV.dial SSV @16:06:27.81 SSV.dial :spit,avoiding: :exhaust @16:06:29.12 SS.reconfigure :default @16:06:29.16 SS.seek 0ml @16:06:32.44 SS.stop @16:06:32.47 Lysing: Adding 2.0ml AqMeOH @16:06:32.48 CBV.dial between CSV and waste1,avoiding: :DBV @16:06:33.99 CTV.dial CSV @16:06:35.33 CSV.dial CSR @16:06:36.12 Pulling 1ml of CSR.AqMeOH @16:06:36.13 CSR.select :AqMeOH @16:06:36.23 CS.reconfigure :default @16:06:36.27 CS.seek 1ml @16:06:39.66 delay 5 seconds @16:06:44.67 CSR.select :air @16:06:44.83 CS.seek 5.999ml @16:06:58.47 delay 2 seconds @16:07:00.48 CSV.dial :waste2 @16:07:01.04 CS.reconfigure :fast @16:07:01.08 CS.seek 0ml @16:07:15.59 CSV.dial CSR @16:07:16.07 Pulling 2ml of CSR.AqMeOH @16:07:16.08 CSR.select :AqMeOH @16:07:16.19 CS.reconfigure :default @16:07:16.23 CS.seek 2ml @16:07:22.13 delay 5 seconds @16:07:27.14 CSR.select :air @16:07:27.29 CS.seek 5ml @16:07:35.78 delay 2 seconds @16:07:37.80 CSV.dial CTV @16:07:38.22 CS.seek 1ml @16:07:49.41 Before lysing Collection Puck Heater at 18.1C/39.1psia @16:07:49.43 Heating Puck.from 4, :sh1 in Collection Clamp to 70C for 10:00 @16:07:49.45 CH.seek 70C,8:00 @16:09:38.82 CH.hold 10:00,70C @16:09:38.99 Begun lysing Collection Puck Heater at 68.1C/46.9psia @16:19:39.00 Ended lysing Collection Puck Heater at 70C/45.5psia @16:19:39.01 CS.seek 0ml @16:19:42.37 CTV.dial between spare and air,avoiding: :intake @16:19:43.41 CSV.dial :air @16:19:43.85 CS.seek 5ml @16:19:57.45 CSV.dial CTV @16:19:57.89 delay 2 seconds @16:19:59.91 CSV.dial :air @16:20:00.36 delay 2 seconds @16:20:02.38 CSV.dial CTV @16:20:02.82 delay 2 seconds @16:20:04.83 CSV.dial :air @16:20:05.27 delay 2 seconds @16:20:07.29 CS.seek 0ml @16:20:21.04 Puck @72.9psia after final lysis pressurization @16:20:21.05 Recovering Lysate into Collection syringe @16:20:21.06 CSV.dial CBV @16:20:22.05 CBV.dial CSV @16:20:22.40 CS.seek 4.999ml @16:20:35.99 delay 5 seconds @16:20:41.00 CTV.dial :air @16:20:41.51 CS.seek 7ml @16:20:47.49 delay 5 seconds @16:20:52.50 CSV.dial :air2 @16:20:52.94 CS.seek 10ml @16:21:01.55 CSV.dial between air and CTV,avoiding: CBV @16:21:02.69 Lysing Complete @16:21:02.76 Storing sh1 puck in Collection Clamp to tube 3 @16:21:02.92 SC.reconfigure :tight @16:21:02.97 SC.seek 3 @16:21:06.58 SC.configure :limp @16:21:06.77 Elbow.reconfigure :tight @16:21:06.81 Elbow.seek 3 @16:21:08.50 Forearm.reconfigure :tight @16:21:08.54 Forearm.seek 3 + 450 counts @16:21:09.54 SE.reconfigure :pick @16:21:09.59 SE.seek :beamBreak @16:21:12.36 SE.configure :default @16:21:12.50 Elbow.reconfigure :limp @16:21:12.54 Forearm.reconfigure :loose @16:21:12.59 Forearm.seek 3 @16:21:12.87 Elbow.hold @16:21:12.93 Elbow.reconfigure :tight @16:21:13.02 SE.seek 5.15 Pucks below Top Plate @16:21:13.47 Forearm.force -20 @16:21:13.52 Forearm.seek 3 + 252 counts @16:21:13.78 Forearm.reconfigure :tight @16:21:13.83 Forearm.seek 3 + 450 counts @16:21:14.05 SE.seek 6.15 Pucks below Top Plate @16:21:14.58 Forearm.reconfigure :loose @16:21:14.62 Forearm.seek :retract @16:21:16.03 Elbow.seek CC @16:21:17.71 Elbow.reconfigure :loose @16:21:17.80 Forearm.seek CC @16:21:18.76 Hand.close @16:21:19.39 CC.seek :open @16:21:24.10 Forearm.seek :retract @16:21:25.03 Elbow.reconfigure :tight @16:21:25.08 Elbow.seek 3 @16:21:26.83 Elbow.reconfigure :limp @16:21:26.87 Forearm.seek 3 @16:21:27.81 Elbow.hold @16:21:27.87 Elbow.reconfigure :tight @16:21:27.91 Hand.open @16:21:28.63 Forearm.force -20 @16:21:28.66 Forearm.seek 3 + 316 counts @16:21:28.95 Forearm.reconfigure :tight @16:21:28.99 Forearm.seek 3 + 450 counts @16:21:29.24 SE.seek :down @16:21:30.35
Forearm.reconfigure :loose @16:21:30.40 Forearm.seek :retract @16:21:31.52 SE.stop @16:21:31.56
Tranferring DA @16:21:31.57 Beginning samplePassC @16:21:31.58 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @16:21:31.77 Elbow.seek FlushPuck::Garage @16:21:33.52 Elbow.reconfigure :loose @16:21:33.62 Forearm.seek :garage @16:21:34.59 Hand.close @16:21:35.25 Forearm.seek :retract @16:21:36.21 Elbow.reconfigure :tight @16:21:36.25 Elbow.seek CC @16:21:39.19 Elbow.reconfigure :loose @16:21:39.28 Forearm.reconfigure :tight @16:21:39.33 Forearm.seek CC @16:21:40.57 CC.seek :closed @16:21:45.08 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.35 @16:21:45.14 Hand.open @16:21:45.82 Forearm.reconfigure :loose @16:21:45.87 Forearm.seek :retract @16:21:46.80 PSV.dial PSR @16:21:48.15 Pulling 1.5ml of PSR.flush @16:21:48.16 PSR.select :flush @16:21:48.28 PS.seek 1.5ml @16:22:04.34 delay 5 seconds @16:22:09.35 PSV.dial :waste1 @16:22:09.82 PS.seek 0ml @16:22:25.92 PSV.dial PSR @16:22:26.38 PSR.select :air @16:22:26.49 PS.seek 2.5ml @16:22:52.87 PRV.dial CBV,avoiding: :ab2 @16:22:52.91
CBV.dial PRV @16:22:54.57 PSV.dial :mixloop @16:22:55.58 CTV.dial :air,avoiding: :intake @16:22:55.60 PTV.dial :PRVmixing @16:22:56.90 PS.seek 0ml @16:23:23.20 PSV.dial :air @16:23:23.77 PS.seek 2.5ml @16:23:50.14 PSV.dial :mixloop @16:23:50.59 PS.seek 0ml @16:24:16.86 PSV.dial :air @16:24:17.34 PS.seek 2.5ml @16:24:43.64 PSV.dial :mixloop @16:24:44.10 PS.seek 0ml @16:25:10.46 Garaging FlushPuck in Collection Clamp @16:25:10.52 Elbow.reconfigure :tight @16:25:11.05 Elbow.seek CC @16:25:11.68 Elbow.reconfigure :loose @16:25:11.77 Forearm.seek CC @16:25:12.78 Hand.close @16:25:13.39 CC.seek :open @16:25:18.00 Forearm.seek :retract @16:25:19.02 Elbow.reconfigure :tight @16:25:19.06 Elbow.seek :garage @16:25:22.07 Elbow.reconfigure :loose @16:25:22.16 Forearm.reconfigure :tight @16:25:22.20 Forearm.seek :garage @16:25:23.26 Hand.open @16:25:23.95 Forearm.reconfigure :loose @16:25:23.99 Forearm.seek :retract @16:25:24.97 PSV.dial :mixloop @16:25:24.98 PTV.dial :PRVmixing @16:25:24.99 PRV.dial CBV @16:25:25.01
CBV.dial PRV @16:25:25.03 CTV.dial :air,avoiding: :intake @16:25:25.05 Loading lyfil puck from tube 4 into Collection Clamp @16:25:25.26 SC.reconfigure :tight @16:25:25.31 SC.seek 4 @16:25:28.88 SC.configure :limp @16:25:29.07 Elbow.reconfigure :tight @16:25:29.11 Elbow.seek 4 @16:25:31.09 Forearm.reconfigure :tight @16:25:31.14 Forearm.seek 4 + 450 counts @16:25:32.16 SE.reconfigure :pick @16:25:32.22 SE.seek :beamBreak @16:25:33.68 SE.configure :default @16:25:33.83 Elbow.seek 4 @16:25:34.01 Forearm.seek 4 + 300 counts @16:25:34.18 Forearm.reconfigure :loose @16:25:34.23 Hand.close @16:25:34.90 SE.seek :down @16:25:35.09
Forearm.seek :retract @16:25:36.01 SE.stop @16:25:36.07
Elbow.seek CC @16:25:37.65 Elbow.reconfigure :loose @16:25:37.74 Forearm.reconfigure :tight @16:25:37.78 Forearm.seek CC @16:25:39.02 CC.seek :closed @16:25:43.53 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.12 @16:25:43.54 Hand.open @16:25:44.20 Forearm.reconfigure :loose @16:25:44.24 Forearm.seek :retract @16:25:45.19 CTV.dial CSV,avoiding: :intake @16:25:46.54 CSV.dial CTV @16:25:46.85 PS.seek 2.5ml @16:26:13.33 CS.seek 4.501ml @16:26:28.29 delay 8 seconds @16:26:36.31 PSV.dial :air @16:26:36.78 delay 2 seconds @16:26:38.80 PSV.dial :waste2 @16:26:39.52 PS.seek 0ml @16:27:05.89 CBV.dial between PRV and waste1 @16:27:06.22 CSV.dial :air @16:27:06.67 CS.seek 10ml @16:27:21.63 CSV.dial CTV @16:27:22.08 CS.seek 0ml @16:27:48.45 CBV.dial :waste1 @16:27:48.75 delay 5 seconds @16:27:53.77 CTV.dial :air,avoiding: :intake @16:27:55.16 Storing lyfil puck in Collection Clamp to tube 3 @16:27:55.79 SC.reconfigure :tight @16:27:55.83 SC.seek 3 @16:27:59.43 SC.configure :limp @16:27:59.62 Elbow.reconfigure :tight @16:27:59.66 Elbow.seek 3 @16:28:01.48 Forearm.reconfigure :tight @16:28:01.52 Forearm.seek 3 + 450 counts @16:28:02.59 SE.reconfigure :pick @16:28:02.64 SE.seek :beamBreak @16:28:05.36 SE.configure :default @16:28:05.50 Elbow.reconfigure :limp @16:28:05.54 Forearm.reconfigure :loose @16:28:05.58 Forearm.seek 3 @16:28:05.82 Elbow.hold @16:28:05.88 Elbow.reconfigure :tight @16:28:05.97 SE.seek 6.12 Pucks below Top Plate @16:28:06.41 Forearm.force -20 @16:28:06.44 Forearm.seek 3 + 298 counts @16:28:06.72 Forearm.reconfigure :tight @16:28:06.76 Forearm.seek 3 + 450 counts @16:28:06.94 SE.seek 7.12 Pucks below Top Plate @16:28:07.43 Forearm.reconfigure :loose @16:28:07.48 Forearm.seek :retract @16:28:08.37 Elbow.seek CC @16:28:10.03 Elbow.reconfigure :loose @16:28:10.12 Forearm.seek CC @16:28:11.06 Hand.close @16:28:11.64 CC.seek :open @16:28:16.35 Forearm.seek :retract @16:28:17.29 Elbow.reconfigure :tight @16:28:17.34 Elbow.seek 3 @16:28:19.10 Elbow.reconfigure :limp @16:28:19.15 Forearm.seek 3 @16:28:20.10 Elbow.hold @16:28:20.16 Elbow.reconfigure :tight @16:28:20.21 Hand.open @16:28:20.87 Forearm.force -20 @16:28:20.91 Forearm.seek 3 + 319 counts @16:28:21.19 Forearm.reconfigure :tight @16:28:21.24 Forearm.seek 3 + 450 counts @16:28:21.50 SE.seek :down @16:28:22.63
Forearm.reconfigure :loose @16:28:22.67 Forearm.seek :retract @16:28:23.69 SE.stop @16:28:23.73
PSV.dial :air @16:28:24.47 PS.seek 2.5ml @16:28:50.86 delay 1 second @16:28:51.88 PSV.dial :mixloop @16:28:52.36 delay 1 second @16:28:53.37 PSV.dial :air @16:28:53.83 delay 1 second @16:28:54.84 PSV.dial :mixloop @16:28:55.29 delay 1 second @16:28:56.31 PSV.dial :air @16:28:56.76 delay 1 second @16:28:57.78 PS.seek 0ml @16:29:24.17 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @16:29:24.34 Elbow.seek FlushPuck::Garage @16:29:26.14 Elbow.reconfigure :loose @16:29:26.23 Forearm.seek :garage @16:29:27.15 Hand.close @16:29:27.87 Forearm.seek :retract @16:29:28.84 Elbow.reconfigure :tight @16:29:28.89 Elbow.seek PC @16:29:30.13 Elbow.reconfigure :loose @16:29:30.21 Forearm.reconfigure :tight @16:29:30.25 Forearm.seek PC @16:29:31.94 PC.seek :closed @16:29:36.58 Processing Clamp onSpring/offSpring for 16/26 samples, on/off torque 1.55 @16:29:36.66 Hand.open @16:29:37.32 Forearm.reconfigure :loose @16:29:37.36 Forearm.seek :retract @16:29:38.32 PRV.dial :air @16:29:38.76 PSV.dial :mixloop @16:29:39.30 PS.seek 0.3ml @16:29:43.17 PSV.dial PSR @16:29:44.38 Pulling 0.4ml of PSR.air @16:29:44.39 PSR.select :air @16:29:44.42 PS.seek 0.7ml @16:29:49.36 delay 3 seconds @16:29:52.37 PSR.select :air @16:29:52.40 PS.seek 2.5ml @16:30:11.51 delay 3 seconds @16:30:14.53 PSV.dial :mixloop @16:30:15.54 PTV.dial :mixing @16:30:16.29 PBV.dial :waste2 @16:30:17.25 PS.seek 0ml @16:30:43.57 Garaging FlushPuck in Processing Clamp @16:30:43.64 Elbow.reconfigure :tight @16:30:43.68 Elbow.seek PC @16:30:43.80 Elbow.reconfigure :loose @16:30:43.88 Forearm.seek PC @16:30:44.83 Hand.close @16:30:45.39 PC.seek :open @16:30:50.09 Forearm.seek :retract @16:30:51.03 Elbow.reconfigure :tight @16:30:51.07 Elbow.seek :garage @16:30:52.41 Elbow.reconfigure :loose @16:30:52.49 Forearm.reconfigure :tight @16:30:52.53 Forearm.seek :garage @16:30:53.55 Hand.open @16:30:54.19 Forearm.reconfigure :loose @16:30:54.23 Forearm.seek :retract @16:30:55.20 Loading da puck from tube 4 into Processing Clamp @16:30:55.41 SC.reconfigure :tight @16:30:55.46 SC.seek 4 @16:30:59.03 SC.configure :limp @16:30:59.22 Elbow.reconfigure :tight @16:30:59.27 Elbow.seek 4 @16:31:01.12 Forearm.reconfigure :tight @16:31:01.17 Forearm.seek 4 + 450 counts @16:31:02.22 SE.reconfigure :pick @16:31:02.27 SE.seek :beamBreak @16:31:03.82 SE.configure :default @16:31:03.96 Elbow.seek 4 @16:31:04.12 Forearm.seek 4 + 300 counts @16:31:04.26 Forearm.reconfigure :loose @16:31:04.30 Hand.close @16:31:04.90 SE.seek :down @16:31:05.08
Forearm.seek :retract @16:31:06.04 Elbow.seek PC @16:31:06.11 SE.stop @16:31:08.55
Elbow.reconfigure :loose @16:31:08.64 Forearm.reconfigure :tight @16:31:08.68 Forearm.seek PC @16:31:09.91 PC.seek :closed @16:31:14.55 Processing Clamp onSpring/offSpring for 16/26 samples, on/off torque 1.58 @16:31:15.04 Hand.open @16:31:15.69 Forearm.reconfigure :loose @16:31:15.73 Forearm.seek :retract @16:31:16.75 Heating Puck.from 4, :da in Processing Clamp to 28C for 2:00:00 @16:31:16.77 PH.seek 28C,5:00 @16:31:51.38 PH.hold 2:00:00,28C @16:31:51.42 delay 1:30 @16:33:21.46 PSR.select :air @16:33:21.48 PSV.dial PSR @16:33:22.58 Pulling 0.5ml of PSR.wash @16:33:22.59 PSR.select :wash @16:33:22.70 PS.seek 0.5ml @16:33:28.57 delay 5 seconds @16:33:33.59 PSR.select :air @16:33:33.70 PS.seek 2ml @16:33:49.88 PTV.dial :puck @16:33:50.60 PSV.dial PTV @16:33:51.35 PBV.dial :waste1 @16:33:52.72 PS.seek 1.5ml @16:33:58.66 delay 3 seconds @16:34:01.68 PBV.dial between PSV and waste1 @16:34:02.05 PS.seek 0ml @16:34:18.26 delay 5:00 @16:39:18.27 recoverPSRreagent08 @16:39:18.28 PTV.dial :puck @16:39:18.29 PBV.dial :waste1 @16:39:18.66 delay 30 seconds @16:39:48.68 PBV.dial between PSV and waste1 @16:39:49.03 PSR.select :air @16:39:49.05 PSV.dial PSR @16:39:49.80 PS.reconfigure :fast @16:39:49.84 PS.seek 2.5ml @16:40:13.28 PSV.dial PTV @16:40:14.16 delay 5 seconds @16:40:19.17 PSV.dial PSR @16:40:19.91 delay 5 seconds @16:40:24.92 PSV.dial PTV @16:40:25.67 delay 5 seconds @16:40:30.69 PSV.dial :air @16:40:31.46 PS.seek 0ml @16:40:54.62 PTV.dial :PRVmixing,via: PRV @16:40:55.94 PSV.dial :mixloop @16:40:56.39 PRV.dial :ab1 @16:40:57.13 PS.reconfigure :slow @16:40:57.17 PS.seek 0.15ml @16:41:00.75 delay 2 seconds @16:41:02.77 CBV.dial PRV @16:41:02.82
PRV.dial CBV @16:41:03.34 PS.seek 0.35ml @16:41:07.94 delay 2 seconds @16:41:09.95 CBV.dial between PRV and PSV @16:41:10.26 PRV.dial :air @16:41:10.71 PS.seek 0.9ml @16:41:22.50 PRV.dial :waste2 @16:41:23.24 PS.seek 0.5ml @16:41:31.97 addPMLReagent08 @16:41:31.98 PSV.dial :air @16:41:32.44 PS.reconfigure :default @16:41:32.48 PS.seek 2ml @16:41:48.84 PSV.dial :PML @16:41:49.29 PTV.dial :mixing @16:41:50.03 PBV.dial :waste1 @16:41:50.43 PS.seek 1.85ml @16:41:52.84 PBV.dial between PSV and waste1 @16:41:53.21 PS.seek 0ml @16:42:12.90 delay 2 seconds @16:42:14.92 Beginning samplePassC @16:42:14.93 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @16:42:15.09 Elbow.reconfigure :tight @16:42:15.13 Elbow.seek FlushPuck::Garage @16:42:16.46 Elbow.reconfigure :loose @16:42:16.55 Forearm.seek :garage @16:42:17.55 Hand.close @16:42:18.24 Forearm.seek :retract @16:42:19.17 Elbow.reconfigure :tight @16:42:19.22 Elbow.seek CC @16:42:22.56 Elbow.reconfigure :loose @16:42:22.65 Forearm.reconfigure :tight @16:42:22.70 Forearm.seek CC @16:42:23.87 CC.seek :closed @16:42:28.41 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.37 @16:42:28.49 Hand.open @16:42:29.19 Forearm.reconfigure :loose @16:42:29.23 Forearm.seek :retract @16:42:30.17 PSV.dial PSR @16:42:31.39 Pulling 1.5ml of PSR.flush @16:42:31.40 PSR.select :flush @16:42:31.52 PS.seek 1.5ml @16:42:47.66 delay 5 seconds @16:42:52.67 PSV.dial :waste1 @16:42:53.14 PS.seek 0ml @16:43:09.28 PSV.dial PSR @16:43:09.73 PSR.select :air @16:43:09.84 PS.seek 2.5ml @16:43:36.16 PRV.dial CBV,avoiding: :ab2 @16:43:36.18
CBV.dial PRV @16:43:37.78 PSV.dial :mixloop @16:43:38.81 CTV.dial :air,avoiding: :intake @16:43:38.82 PTV.dial :PRVmixing @16:43:39.56 PS.seek 0ml @16:44:05.87 PSV.dial :air @16:44:06.44 PS.seek 2.5ml @16:44:32.74 PSV.dial :mixloop @16:44:33.19 PS.seek 0ml @16:44:59.55 PSV.dial :air @16:45:00.02 PS.seek 2.5ml @16:45:26.34 PSV.dial :mixloop @16:45:26.80 PS.seek 0ml @16:45:53.12 Garaging FlushPuck in Collection Clamp @16:45:53.19 Elbow.reconfigure :tight @16:45:53.23 Elbow.seek CC @16:45:53.49 Elbow.reconfigure :loose @16:45:53.58 Forearm.seek CC @16:45:54.53 Hand.close @16:45:55.12 CC.seek :open @16:45:59.76 Forearm.seek :retract @16:46:00.72 Elbow.reconfigure :tight @16:46:00.76 Elbow.seek :garage @16:46:03.66 Elbow.reconfigure :loose @16:46:03.75 Forearm.reconfigure :tight @16:46:03.79 Forearm.seek :garage @16:46:04.80 Hand.open @16:46:05.43 Forearm.reconfigure :loose @16:46:05.47 Forearm.seek :retract @16:46:06.44 delayUntil "16:50:14.91" @16:46:06.46 Whole Cell Archive sampling 1000ml @16:46:06.48 Loading wcr puck from tube 4 into Collection Clamp @16:46:06.70 SC.reconfigure :tight @16:46:06.74 SC.seek 4 @16:46:06.83 SC.configure :limp @16:46:07.03 Elbow.reconfigure :tight @16:46:07.08 Elbow.seek 4 @16:46:08.99 Forearm.reconfigure :tight @16:46:09.03 Forearm.seek 4 + 450 counts @16:46:10.26 SE.reconfigure :pick @16:46:10.36 SE.seek :beamBreak @16:46:11.99 SE.configure :default @16:46:12.14 Elbow.seek 4 @16:46:12.32 Forearm.seek 4 + 300 counts @16:46:12.96 Forearm.reconfigure :loose @16:46:13.02 Hand.close @16:46:13.64 SE.seek :down @16:46:13.84 Forearm.seek :retract @16:46:14.80 Elbow.seek CC @16:46:14.86 SE.stop @16:46:16.43 Elbow.reconfigure :loose @16:46:16.53 Forearm.reconfigure :tight @16:46:16.57 Forearm.seek CC @16:46:17.82 CC.seek :closed @16:46:22.34 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.22 @16:46:22.35 Hand.open @16:46:23.06 Forearm.reconfigure :loose @16:46:23.10 Forearm.seek :retract @16:46:24.05 collectSample 1000ml w/bubble point of 35psi @16:46:24.06 Priming 7ml @16:46:24.07 Intake.select :close @16:46:24.08 Exhaust.select :close @16:46:24.09 SS.hold @16:46:24.29 Calibrating pressure sensors @16:46:24.30 CTV.dial :air @16:46:24.31 SSV.dial :spit,avoiding: :exhaust @16:46:24.33 SS.reconfigure :fast @16:46:24.38 SS.seek 5ml @16:46:27.21 SSV.dial CBV @16:46:27.24 CBV.dial SSV @16:46:28.55 delay 3.8 seconds @16:46:32.48 SSV.dial :spit @16:46:33.79 SS.seek 0ml @16:46:36.50 SSV.dial CBV @16:46:37.81 CTV.dial :intake @16:46:38.58 SSV.dial CBV @16:46:38.60 CBV.dial SSV @16:46:38.62 Allowing 40s for 7.0ml of remaining 7.0ml @16:46:38.63 Intake.select :open @16:46:40.78 Intake @15.0psia, vacuum from 9.5 to 12.5psi @16:46:40.80 SS.reconfigure :inhaling 2.20ml/s @16:46:40.84 SS.seek 7ml @16:46:47.39 Stroke completed -- waiting for pressure to equalize ... @16:46:47.40 SS.reconfigure :exhale @16:46:47.44 SS.hold @16:47:00.08 Intake.select :close @16:47:00.22 SSV.dial :exhaust @16:47:00.98 Exhaust.select :open @16:47:01.09 SS.seek 0ml @16:47:05.44 Exhaust.select :close @16:47:05.54 Sampling 1000.0ml @16:47:05.55 Intake.select :close @16:47:05.56 Exhaust.select :close @16:47:05.58 SS.reconfigure :default @16:47:05.62 SS.hold @16:47:05.81 CTV.dial :intake @16:47:05.83 SSV.dial CBV @16:47:05.86 CBV.dial SSV @16:47:06.61 Allowing 130s for 25.0ml of remaining 1000.0ml @16:47:06.62 Intake.select :open @16:47:08.77 Intake @15.5psia, vacuum from 10.0 to 13.0psi @16:47:08.79 SS.reconfigure :inhaling 2.20ml/s @16:47:08.83 SS.seek 25ml @16:47:16.63 Vacuum too high -- waiting up to 40 seconds @16:47:16.64 SS.reconfigure :exhale @16:47:16.69 SS.hold @16:47:23.67 Intake @16.3psia, vacuum from 10.0 to 13.0psi @16:47:23.69 SS.reconfigure :inhaling 1.98ml/s @16:47:23.73 SS.seek 25ml @16:47:28.70 Vacuum too high -- waiting up to 40 seconds @16:47:28.71 SS.reconfigure :exhale @16:47:28.75 SS.hold @16:47:33.15 Intake @16.8psia, vacuum from 10.0 to 13.0psi @16:47:33.17 SS.reconfigure :inhaling 1.79ml/s @16:47:33.22 SS.seek 25ml @16:47:37.59 Stroke completed -- waiting for pressure to equalize ... @16:47:37.60 SS.reconfigure :exhale @16:47:37.65 SS.hold @16:47:44.04 Intake.select :close @16:47:44.18 SSV.dial :exhaust @16:47:44.92 Exhaust.select :open @16:47:45.03 SS.seek 0ml @16:47:53.81 Exhaust.select :close @16:47:53.92 SSV.dial CBV @16:47:53.95 CBV.dial SSV @16:47:54.71 Allowing 130s for 25.0ml of remaining 975.0ml @16:47:54.72 Intake.select :open @16:47:56.87 Intake @16.7psia, vacuum from 10.0 to 13.0psi @16:47:56.89 SS.reconfigure :inhaling 1.79ml/s @16:47:56.93 SS.seek 25ml @16:48:03.75 Vacuum too high -- waiting up to 40 seconds @16:48:03.77 SS.reconfigure :exhale @16:48:03.81 SS.hold @16:48:06.50 Intake @15.7psia, vacuum from 10.0 to 13.0psi @16:48:06.52 SS.reconfigure :inhaling 1.61ml/s @16:48:06.56 SS.seek 25ml @16:48:18.32 Vacuum too high -- waiting up to 40 seconds @16:48:18.33 SS.reconfigure :exhale @16:48:18.37 SS.hold @16:48:26.95 Intake @17.6psia, vacuum from 10.0 to 13.0psi @16:48:26.97 SS.reconfigure :inhaling 1.45ml/s @16:48:27.01 SS.seek 25ml @16:48:28.03 Stroke completed -- waiting for pressure to equalize ... @16:48:28.04 SS.reconfigure :exhale @16:48:28.08 SS.hold @16:48:31.08 Intake.select :close @16:48:31.72 SSV.dial :exhaust @16:48:32.48 Exhaust.select :open @16:48:32.59 SS.seek 0ml @16:48:41.34 Exhaust.select :close @16:48:41.45 SSV.dial CBV @16:48:41.47 CBV.dial SSV @16:48:42.25 Allowing 130s for 25.0ml of remaining 950.0ml @16:48:42.26 Intake.select :open @16:48:44.42 Intake @16.7psia, vacuum from 10.0 to 13.0psi @16:48:44.44 SS.reconfigure :inhaling 1.45ml/s @16:48:44.48 SS.seek 25ml @16:48:53.04 Vacuum too high -- waiting up to 40 seconds @16:48:53.05 SS.reconfigure :exhale @16:48:53.09 SS.hold @16:48:55.69 Intake @15.6psia, vacuum from 10.0 to 13.0psi @16:48:55.71 SS.reconfigure :inhaling 1.30ml/s @16:48:55.76 SS.seek 25ml @16:49:09.34 Stroke completed -- waiting for pressure to equalize ... @16:49:09.35 SS.reconfigure :exhale @16:49:09.39 SS.hold @16:49:20.51 Intake.select :close @16:49:20.65 SSV.dial :exhaust @16:49:21.40 Exhaust.select :open @16:49:21.51 SS.seek 0ml @16:49:30.54 Exhaust.select :close @16:49:30.65 SSV.dial CBV @16:49:30.67 CBV.dial SSV @16:49:31.45 Allowing 130s for 25.0ml of remaining 925.0ml @16:49:31.46 Intake.select :open @16:49:33.62 Intake @16.4psia, vacuum from 10.0 to 13.0psi @16:49:33.64 SS.reconfigure :inhaling 1.30ml/s @16:49:33.68 SS.seek 25ml @16:49:44.25 Vacuum too high -- waiting up to 40 seconds @16:49:44.27 SS.reconfigure :exhale @16:49:44.31 SS.hold @16:49:48.56 Intake @16.5psia, vacuum from 10.0 to 13.0psi @16:49:48.58 SS.reconfigure :inhaling 1.17ml/s @16:49:48.62 SS.seek 25ml @16:50:00.41 Vacuum too high -- waiting up to 40 seconds @16:50:00.42 SS.reconfigure :exhale @16:50:00.46 SS.hold @16:50:06.15 Intake @16.9psia, vacuum from 10.0 to 13.0psi @16:50:06.17 SS.reconfigure :inhaling 1.06ml/s @16:50:06.21 SS.seek 25ml @16:50:07.07 Stroke completed -- waiting for pressure to equalize ... @16:50:07.08 SS.reconfigure :exhale @16:50:07.13 SS.hold @16:50:10.03 Intake.select :close @16:50:10.18 SSV.dial :exhaust @16:50:10.93 Exhaust.select :open @16:50:11.55 SS.seek 0ml @16:50:14.92 recoverPRVreagent08 @16:50:14.93 PTV.dial :mixing @16:50:15.68 PBV.dial :waste1 @16:50:16.05 delay 30 seconds @16:50:20.64 Exhaust.select :close @16:50:20.75 SSV.dial CBV @16:50:20.77 CBV.dial SSV @16:50:21.53 Allowing 130s for 25.0ml of remaining 900.0ml @16:50:21.54 Intake.select :open @16:50:23.70 Intake @16.5psia, vacuum from 10.0 to 13.0psi @16:50:23.72 SS.reconfigure :inhaling 1.06ml/s @16:50:23.77 SS.seek 25ml @16:50:39.69 Vacuum too high -- waiting up to 40 seconds @16:50:39.70 SS.reconfigure :exhale @16:50:39.75 SS.hold @16:50:43.92 Intake @16.5psia, vacuum from 10.0 to 13.0psi @16:50:43.94 SS.reconfigure :inhaling 0.95ml/s @16:50:43.98 SS.seek 25ml @16:50:46.07 PBV.dial between PSV and waste1 @16:50:46.44 PSV.dial :air @16:50:46.92 PS.reconfigure :fast @16:50:46.96 PS.seek 2.5ml @16:50:55.38 Stroke completed -- waiting for pressure to equalize ... @16:50:55.40 SS.reconfigure :exhale @16:50:55.44 SS.hold @16:51:02.82 Intake.select :close @16:51:02.96 SSV.dial :exhaust @16:51:03.70 Exhaust.select :open @16:51:03.81 SS.seek 0ml @16:51:10.64 PSV.dial :mixloop @16:51:11.11 delay 2 seconds @16:51:12.63 Exhaust.select :close @16:51:12.74 SSV.dial CBV @16:51:12.76 CBV.dial SSV @16:51:13.13 PSV.dial :air @16:51:13.53 Allowing 130s for 25.0ml of remaining 875.0ml @16:51:13.54 Intake.select :open @16:51:13.61 delay 2 seconds @16:51:15.63 PSV.dial :mixloop @16:51:15.72 Intake @16.4psia, vacuum from 10.0 to 13.0psi @16:51:15.74 SS.reconfigure :inhaling 0.95ml/s @16:51:15.78 SS.seek 25ml @16:51:16.10 delay 2 seconds @16:51:18.11 PSV.dial :air @16:51:18.59 PS.seek 0ml @16:51:39.78 Vacuum too high -- waiting up to 40 seconds @16:51:39.80 SS.reconfigure :exhale @16:51:39.84 SS.hold @16:51:41.89 washCycleDA #1 of 2 @16:51:41.90 PSR.select :air @16:51:41.92 PSV.dial PSR @16:51:43.29 Pulling 0.5ml of PSR.wash @16:51:43.30 PSR.select :wash @16:51:43.47 PS.reconfigure :default @16:51:43.52 PS.seek 0.5ml @16:51:44.13 Intake @16.1psia, vacuum from 10.0 to 13.0psi @16:51:44.14 SS.reconfigure :inhaling 0.87ml/s @16:51:44.19 SS.seek 25ml @16:51:49.49 delay 5 seconds @16:51:50.15 Stroke completed -- waiting for pressure to equalize ... @16:51:50.16 SS.reconfigure :exhale @16:51:50.20 SS.hold @16:51:54.81 PSR.select :air @16:51:54.92 PS.seek 2ml @16:51:56.34 Intake.select :close @16:51:56.48 SSV.dial :exhaust @16:51:57.24 Exhaust.select :open @16:51:57.35 SS.seek 0ml @16:52:06.29 Exhaust.select :close @16:52:06.40 SSV.dial CBV @16:52:06.43 CBV.dial SSV @16:52:07.18 Allowing 130s for 25.0ml of remaining 850.0ml @16:52:07.20 Intake.select :open @16:52:09.35 Intake @16.2psia, vacuum from 10.0 to 13.0psi @16:52:09.37 SS.reconfigure :inhaling 0.87ml/s @16:52:09.41 SS.seek 25ml @16:52:11.14 PTV.dial :puck @16:52:11.89 PSV.dial PTV @16:52:12.75 PBV.dial :waste1 @16:52:13.19 PS.seek 1.5ml @16:52:19.17 delay 3 seconds @16:52:22.19 PBV.dial between PSV and waste1 @16:52:22.57 PS.seek 0ml @16:52:38.74 delay 2:00 @16:52:40.55 Stroke completed -- waiting for pressure to equalize ... @16:52:40.57 SS.reconfigure :exhale @16:52:40.61 SS.hold @16:52:50.30 Intake.select :close @16:52:50.44 SSV.dial :exhaust @16:52:51.20 Exhaust.select :open @16:52:51.31 SS.seek 0ml @16:53:00.16 Exhaust.select :close @16:53:00.27 SSV.dial CBV @16:53:00.29 CBV.dial SSV @16:53:01.06 Allowing 130s for 25.0ml of remaining 825.0ml @16:53:01.07 Intake.select :open @16:53:03.22 Intake @16.2psia, vacuum from 10.0 to 13.0psi @16:53:03.24 SS.reconfigure :inhaling 0.87ml/s @16:53:03.28 SS.seek 25ml @16:53:31.62 Vacuum too high -- waiting up to 40 seconds @16:53:31.64 SS.reconfigure :exhale @16:53:31.68 SS.hold @16:53:36.57 Intake @15.5psia, vacuum from 10.0 to 13.0psi @16:53:36.58 SS.reconfigure :inhaling 0.78ml/s @16:53:36.63 SS.seek 25ml @16:53:40.29 Stroke completed -- waiting for pressure to equalize ... @16:53:40.30 SS.reconfigure :exhale @16:53:40.34 SS.hold @16:53:45.89 Intake.select :close @16:53:46.03 SSV.dial :exhaust @16:53:46.79 Exhaust.select :open @16:53:46.91 SS.seek 0ml @16:53:55.72 Exhaust.select :close @16:53:55.83 SSV.dial CBV @16:53:55.85 CBV.dial SSV @16:53:56.62 Allowing 130s for 25.0ml of remaining 800.0ml @16:53:56.63 Intake.select :open @16:53:58.79 Intake @16.1psia, vacuum from 10.0 to 13.0psi @16:53:58.81 SS.reconfigure :inhaling 0.78ml/s @16:53:58.85 SS.seek 25ml @16:54:33.41 Stroke completed -- waiting for pressure to equalize ... @16:54:33.42 SS.reconfigure :exhale @16:54:33.94 SS.hold @16:54:38.79 recoverPSRreagent08 @16:54:38.80 PTV.dial :puck @16:54:38.81 PBV.dial :waste1 @16:54:39.17 delay 30 seconds @16:54:43.73 Intake.select :close @16:54:43.88 SSV.dial :exhaust @16:54:44.65 Exhaust.select :open @16:54:44.76 SS.seek 0ml @16:54:53.63 Exhaust.select :close @16:54:53.74 SSV.dial CBV @16:54:53.76 CBV.dial SSV @16:54:54.54 Allowing 130s for 25.0ml of remaining 775.0ml @16:54:54.55 Intake.select :open @16:54:56.71 Intake @16.1psia, vacuum from 10.0 to 13.0psi @16:54:56.73 SS.reconfigure :inhaling 0.78ml/s @16:54:56.77 SS.seek 25ml @16:55:09.20 PBV.dial between PSV and waste1 @16:55:09.56 PSR.select :air @16:55:09.58 PSV.dial PSR @16:55:10.33 PS.reconfigure :fast @16:55:10.38 PS.seek 2.5ml @16:55:31.60 Stroke completed -- waiting for pressure to equalize ... @16:55:31.61 SS.reconfigure :exhale @16:55:31.65 SS.hold @16:55:34.26 PSV.dial PTV @16:55:35.02 delay 5 seconds @16:55:40.10 PSV.dial PSR @16:55:40.84 delay 5 seconds @16:55:42.74 Intake.select :close @16:55:42.88 SSV.dial :exhaust @16:55:43.65 Exhaust.select :open @16:55:43.76 SS.seek 0ml @16:55:45.86 PSV.dial PTV @16:55:46.73 delay 5 seconds @16:55:51.75 PSV.dial :air @16:55:52.64 PS.seek 0ml @16:55:52.86 Exhaust.select :close @16:55:52.97 SSV.dial CBV @16:55:52.99 CBV.dial SSV @16:55:53.76 Allowing 130s for 25.0ml of remaining 750.0ml @16:55:53.77 Intake.select :open @16:55:55.92 Intake @16.1psia, vacuum from 10.0 to 13.0psi @16:55:55.94 SS.reconfigure :inhaling 0.78ml/s @16:55:55.98 SS.seek 25ml @16:56:16.14 washCycleDA #2 of 2 @16:56:16.15 PSR.select :air @16:56:16.16 PSV.dial PSR @16:56:17.53 Pulling 0.5ml of PSR.wash @16:56:17.54 PSR.select :wash @16:56:17.66 PS.reconfigure :default @16:56:17.70 PS.seek 0.5ml @16:56:23.83 delay 5 seconds @16:56:28.85 PSR.select :air @16:56:28.96 PS.seek 2ml @16:56:30.03 Stroke completed -- waiting for pressure to equalize ... @16:56:30.05 SS.reconfigure :exhale @16:56:30.09 SS.hold @16:56:43.13 Intake.select :close @16:56:43.27 SSV.dial :exhaust @16:56:44.02 Exhaust.select :open @16:56:44.13 SS.seek 0ml @16:56:45.08 PTV.dial :puck @16:56:45.10 PSV.dial PTV @16:56:45.86 PBV.dial :waste1 @16:56:46.29 PS.seek 1.5ml @16:56:52.20 delay 3 seconds @16:56:53.22 Exhaust.select :close @16:56:53.33 SSV.dial CBV @16:56:53.35 CBV.dial SSV @16:56:54.12 Allowing 130s for 25.0ml of remaining 725.0ml @16:56:54.13 Intake.select :open @16:56:55.22 PBV.dial between PSV and waste1 @16:56:55.58 PS.seek 0ml @16:56:56.28 Intake @16.1psia, vacuum from 10.0 to 13.0psi @16:56:56.30 SS.reconfigure :inhaling 0.78ml/s @16:56:56.34 SS.seek 25ml @16:57:11.73 delay 2:00 @16:57:20.43 Vacuum too high -- waiting up to 40 seconds @16:57:20.45 SS.reconfigure :exhale @16:57:20.49 SS.hold @16:57:26.99 Intake @15.9psia, vacuum from 10.0 to 13.0psi @16:57:27.01 SS.reconfigure :inhaling 0.70ml/s @16:57:27.05 SS.seek 25ml @16:57:38.53 Stroke completed -- waiting for pressure to equalize ... @16:57:38.55 SS.reconfigure :exhale @16:57:38.59 SS.hold @16:57:48.27 Intake.select :close @16:57:48.41 SSV.dial :exhaust @16:57:49.18 Exhaust.select :open @16:57:49.29 SS.seek 0ml @16:57:58.11 Exhaust.select :close @16:57:58.22 SSV.dial CBV @16:57:58.24 CBV.dial SSV @16:57:59.02 Allowing 130s for 25.0ml of remaining 700.0ml @16:57:59.03 Intake.select :open @16:58:01.19 Intake @16.0psia, vacuum from 10.0 to 13.0psi @16:58:01.20 SS.reconfigure :inhaling 0.70ml/s @16:58:01.25 SS.seek 25ml @16:58:39.28 Stroke completed -- waiting for pressure to equalize ... @16:58:39.29 SS.reconfigure :exhale @16:58:39.34 SS.hold @16:58:53.69 Intake.select :close @16:58:53.83 SSV.dial :exhaust @16:58:54.60 Exhaust.select :open @16:58:54.71 SS.seek 0ml @16:59:03.83 Exhaust.select :close @16:59:03.94 SSV.dial CBV @16:59:03.97 CBV.dial SSV @16:59:04.72 Allowing 130s for 25.0ml of remaining 675.0ml @16:59:05.20 Intake.select :open @16:59:07.38 Intake @16.0psia, vacuum from 10.0 to 13.0psi @16:59:07.40 SS.reconfigure :inhaling 0.70ml/s @16:59:07.44 SS.seek 25ml @16:59:11.75 recoverPSRreagent08 @16:59:11.76 PTV.dial :puck @16:59:11.77 PBV.dial :waste1 @16:59:12.12 delay 30 seconds @16:59:42.14 PBV.dial between PSV and waste1 @16:59:42.49 PSR.select :air @16:59:42.51 PSV.dial PSR @16:59:42.90 Vacuum too high -- waiting up to 40 seconds @16:59:42.91 SS.reconfigure :exhale @16:59:42.96 SS.hold @16:59:43.39 PS.reconfigure :fast @16:59:43.43 PS.seek 2.5ml @16:59:51.54 Intake @15.9psia, vacuum from 10.0 to 13.0psi @16:59:51.55 SS.reconfigure :inhaling 0.63ml/s @16:59:51.60 SS.seek 25ml @16:59:53.92 Stroke completed -- waiting for pressure to equalize ... @16:59:53.93 SS.reconfigure :exhale @16:59:53.98 SS.hold @17:00:01.36 Intake.select :close @17:00:01.51 SSV.dial :exhaust @17:00:02.27 Exhaust.select :open @17:00:02.38 SS.seek 0ml @17:00:07.35 PSV.dial PTV @17:00:08.21 delay 5 seconds @17:00:11.25 Exhaust.select :close @17:00:11.36 SSV.dial CBV @17:00:11.40 CBV.dial SSV @17:00:12.13 Allowing 130s for 25.0ml of remaining 650.0ml @17:00:12.14 Intake.select :open @17:00:13.23 PSV.dial PSR @17:00:13.96 delay 5 seconds @17:00:14.29 Intake @16.0psia, vacuum from 10.0 to 13.0psi @17:00:14.31 SS.reconfigure :inhaling 0.63ml/s @17:00:14.35 SS.seek 25ml @17:00:18.98 PSV.dial PTV @17:00:19.73 delay 5 seconds @17:00:24.75 PSV.dial :air @17:00:25.52 PS.seek 0ml @17:00:48.60 getPMLReagent08 @17:00:48.61 PRV.dial :air @17:00:49.05 delay 5 seconds @17:00:54.07 PTV.dial :PRVmixing @17:00:55.38 delay 5 seconds @17:00:56.59 Stroke completed -- waiting for pressure to equalize ... @17:00:56.60 SS.reconfigure :exhale @17:00:56.65 SS.hold @17:01:00.40 PRV.dial :ab2 @17:01:00.87 PTV.dial :PRVmixing @17:01:00.88 PSV.dial :mixloop @17:01:01.40 PS.reconfigure :default @17:01:01.44 PS.seek 0.3ml @17:01:05.38 delay 2 seconds @17:01:07.40 PRV.dial :air @17:01:07.91 PS.seek 0.55ml @17:01:10.92 Intake.select :close @17:01:11.06 SSV.dial :exhaust @17:01:11.63 addPMLReagent08 @17:01:11.64 PSV.dial :air @17:01:12.11 PS.seek 2ml @17:01:12.33 Exhaust.select :open @17:01:12.44 SS.seek 0ml @17:01:21.22 Exhaust.select :close @17:01:21.32 SSV.dial CBV @17:01:21.36 CBV.dial SSV @17:01:22.10 Allowing 130s for 25.0ml of remaining 625.0ml @17:01:22.11 Intake.select :open @17:01:24.26 Intake @16.0psia, vacuum from 10.0 to 13.0psi @17:01:24.28 SS.reconfigure :inhaling 0.63ml/s @17:01:24.33 SS.seek 25ml @17:01:27.76 PSV.dial :PML @17:01:28.23 PTV.dial :mixing @17:01:28.98 PBV.dial :waste1 @17:01:29.41 PS.seek 1.85ml @17:01:31.85 PBV.dial between PSV and waste1 @17:01:32.23 PS.seek 0ml @17:01:51.91 delay 2 seconds @17:01:53.93 delay 8:00 @17:02:01.27 Vacuum too high -- waiting up to 40 seconds @17:02:01.29 SS.reconfigure :exhale @17:02:01.33 SS.hold @17:02:08.82 Intake @15.5psia, vacuum from 10.0 to 13.0psi @17:02:08.84 SS.reconfigure :inhaling 0.57ml/s @17:02:08.88 SS.seek 25ml @17:02:14.79 Stroke completed -- waiting for pressure to equalize ... @17:02:14.80 SS.reconfigure :exhale @17:02:14.85 SS.hold @17:02:24.50 Intake.select :close @17:02:24.64 SSV.dial :exhaust @17:02:25.39 Exhaust.select :open @17:02:25.50 SS.seek 0ml @17:02:34.70 Exhaust.select :close @17:02:34.81 SSV.dial CBV @17:02:34.83 CBV.dial SSV @17:02:35.59 Allowing 130s for 25.0ml of remaining 600.0ml @17:02:35.61 Intake.select :open @17:02:37.76 Intake @16.1psia, vacuum from 10.0 to 13.0psi @17:02:37.78 SS.reconfigure :inhaling 0.57ml/s @17:02:37.82 SS.seek 25ml @17:03:23.00 Stroke completed -- waiting for pressure to equalize ... @17:03:23.01 SS.reconfigure :exhale @17:03:23.05 SS.hold @17:03:41.38 Intake.select :close @17:03:41.52 SSV.dial :exhaust @17:03:42.26 Exhaust.select :open @17:03:42.37 SS.seek 0ml @17:03:51.23 Exhaust.select :close @17:03:51.33 SSV.dial CBV @17:03:51.84 CBV.dial SSV @17:03:52.62 Allowing 130s for 25.0ml of remaining 575.0ml @17:03:52.64 Intake.select :open @17:03:54.79 Intake @16.1psia, vacuum from 10.0 to 13.0psi @17:03:54.81 SS.reconfigure :inhaling 0.57ml/s @17:03:54.85 SS.seek 25ml @17:04:26.11 Vacuum too high -- waiting up to 40 seconds @17:04:26.12 SS.reconfigure :exhale @17:04:26.17 SS.hold @17:04:33.66 Intake @16.0psia, vacuum from 10.0 to 13.0psi @17:04:33.68 SS.reconfigure :inhaling 0.51ml/s @17:04:33.73 SS.seek 25ml @17:04:50.79 Stroke completed -- waiting for pressure to equalize ... @17:04:50.80 SS.reconfigure :exhale @17:04:50.84 SS.hold @17:05:05.23 Intake.select :close @17:05:05.37 SSV.dial :exhaust @17:05:06.12 Exhaust.select :open @17:05:06.23 SS.seek 0ml @17:05:15.32 Exhaust.select :close @17:05:15.43 SSV.dial CBV @17:05:15.45 CBV.dial SSV @17:05:16.21 Allowing 130s for 25.0ml of remaining 550.0ml @17:05:16.23 Intake.select :open @17:05:18.38 Intake @16.1psia, vacuum from 10.0 to 13.0psi @17:05:18.40 SS.reconfigure :inhaling 0.51ml/s @17:05:18.45 SS.seek 25ml @17:06:02.89 Vacuum too high -- waiting up to 40 seconds @17:06:02.91 SS.reconfigure :exhale @17:06:02.95 SS.hold @17:06:12.74 Intake @16.3psia, vacuum from 10.0 to 13.0psi @17:06:12.76 SS.reconfigure :inhaling 0.47ml/s @17:06:12.80 SS.seek 25ml @17:06:19.85 Stroke completed -- waiting for pressure to equalize ... @17:06:19.86 SS.reconfigure :exhale @17:06:19.90 SS.hold @17:06:31.02 Intake.select :close @17:06:31.16 SSV.dial :exhaust @17:06:31.90 Exhaust.select :open @17:06:32.01 SS.seek 0ml @17:06:41.57 Exhaust.select :close @17:06:41.68 SSV.dial CBV @17:06:41.71 CBV.dial SSV @17:06:42.45 Allowing 130s for 25.0ml of remaining 525.0ml @17:06:42.46 Intake.select :open @17:06:44.62 Intake @16.2psia, vacuum from 10.0 to 13.0psi @17:06:44.64 SS.reconfigure :inhaling 0.47ml/s @17:06:44.68 SS.seek 25ml @17:07:28.11 Vacuum too high -- waiting up to 40 seconds @17:07:28.12 SS.reconfigure :exhale @17:07:28.16 SS.hold @17:07:36.72 Intake @15.9psia, vacuum from 10.0 to 13.0psi @17:07:36.74 SS.reconfigure :inhaling 0.43ml/s @17:07:36.78 SS.seek 25ml @17:07:50.10 Stroke completed -- waiting for pressure to equalize ... @17:07:50.11 SS.reconfigure :exhale @17:07:50.15 SS.hold @17:08:04.51 Intake.select :close @17:08:04.66 SSV.dial :exhaust @17:08:05.40 Exhaust.select :open @17:08:05.51 SS.seek 0ml @17:08:14.54 Exhaust.select :close @17:08:14.64 SSV.dial CBV @17:08:14.67 CBV.dial SSV @17:08:15.41 Allowing 130s for 25.0ml of remaining 500.0ml @17:08:15.43 Intake.select :open @17:08:17.58 Intake @16.0psia, vacuum from 10.0 to 13.0psi @17:08:17.60 SS.reconfigure :inhaling 0.43ml/s @17:08:17.64 SS.seek 25ml @17:09:19.25 Stroke completed -- waiting for pressure to equalize ... @17:09:19.26 SS.reconfigure :exhale @17:09:19.31 SS.hold @17:09:39.61 Intake.select :close @17:09:39.75 SSV.dial :exhaust @17:09:40.49 Exhaust.select :open @17:09:40.60 SS.seek 0ml @17:09:49.67 Exhaust.select :close @17:09:49.78 SSV.dial CBV @17:09:49.80 CBV.dial SSV @17:09:50.55 Allowing 130s for 25.0ml of remaining 475.0ml @17:09:50.57 Intake.select :open @17:09:52.72 Intake @16.1psia, vacuum from 10.0 to 13.0psi @17:09:52.74 SS.reconfigure :inhaling 0.43ml/s @17:09:52.78 SS.seek 25ml @17:09:53.95 recoverPRVreagent08 @17:09:53.96 PTV.dial :mixing @17:09:53.97 PBV.dial :waste1 @17:09:54.33 delay 30 seconds @17:10:24.36 PBV.dial between PSV and waste1 @17:10:24.73 PSV.dial :air @17:10:25.71 PS.reconfigure :fast @17:10:25.75 PS.seek 2.5ml @17:10:49.68 PSV.dial :mixloop @17:10:50.15 delay 2 seconds @17:10:52.17 PSV.dial :air @17:10:52.64 delay 2 seconds @17:10:54.06 Stroke completed -- waiting for pressure to equalize ... @17:10:54.08 SS.reconfigure :exhale @17:10:54.12 SS.hold @17:10:54.65 PSV.dial :mixloop @17:10:55.12 delay 2 seconds @17:10:57.14 PSV.dial :air @17:10:57.61 PS.seek 0ml @17:11:18.50 Intake.select :close @17:11:18.65 SSV.dial :exhaust @17:11:19.41 Exhaust.select :open @17:11:19.52 SS.seek 0ml @17:11:20.78 washCycleDA #1 of 3 @17:11:20.79 PSR.select :air @17:11:20.80 PSV.dial PSR @17:11:22.17 Pulling 0.5ml of PSR.wash @17:11:22.18 PSR.select :wash @17:11:22.29 PS.reconfigure :default @17:11:22.34 PS.seek 0.5ml @17:11:28.28 delay 5 seconds @17:11:28.36 Exhaust.select :close @17:11:28.47 SSV.dial CBV @17:11:28.49 CBV.dial SSV @17:11:29.27 Allowing 130s for 25.0ml of remaining 450.0ml @17:11:29.28 Intake.select :open @17:11:31.44 Intake @16.1psia, vacuum from 10.0 to 13.0psi @17:11:31.45 SS.reconfigure :inhaling 0.43ml/s @17:11:31.50 SS.seek 25ml @17:11:33.29 PSR.select :air @17:11:33.41 PS.seek 2ml @17:11:49.52 PTV.dial :puck @17:11:50.26 PSV.dial PTV @17:11:51.01 PBV.dial :waste1 @17:11:51.43 PS.seek 1.5ml @17:11:57.37 delay 3 seconds @17:12:00.39 PBV.dial between PSV and waste1 @17:12:00.77 PS.seek 0ml @17:12:16.90 delay 2:00 @17:12:18.04 Vacuum too high -- waiting up to 40 seconds @17:12:18.05 SS.reconfigure :exhale @17:12:18.10 SS.hold @17:12:30.83 Intake @16.4psia, vacuum from 10.0 to 13.0psi @17:12:30.84 SS.reconfigure :inhaling 0.38ml/s @17:12:30.89 SS.seek 25ml @17:12:48.06 Stroke completed -- waiting for pressure to equalize ... @17:12:48.07 SS.reconfigure :exhale @17:12:48.11 SS.hold @17:13:06.97 Intake.select :close @17:13:07.11 SSV.dial :exhaust @17:13:07.88 Exhaust.select :open @17:13:07.99 SS.seek 0ml @17:13:16.84 Exhaust.select :close @17:13:16.95 SSV.dial CBV @17:13:16.97 CBV.dial SSV @17:13:17.75 Allowing 130s for 25.0ml of remaining 425.0ml @17:13:17.76 Intake.select :open @17:13:19.92 Intake @16.2psia, vacuum from 10.0 to 13.0psi @17:13:19.94 SS.reconfigure :inhaling 0.38ml/s @17:13:19.98 SS.seek 25ml @17:14:16.92 recoverPSRreagent08 @17:14:16.93 PTV.dial :puck @17:14:16.95 PBV.dial :waste1 @17:14:17.31 delay 30 seconds @17:14:21.38 Vacuum too high -- waiting up to 40 seconds @17:14:21.40 SS.reconfigure :exhale @17:14:21.44 SS.hold @17:14:35.88 Intake @17.0psia, vacuum from 10.0 to 13.0psi @17:14:35.90 SS.reconfigure :inhaling 0.34ml/s @17:14:35.94 SS.seek 25ml @17:14:46.48 Stroke completed -- waiting for pressure to equalize ... @17:14:46.49 SS.reconfigure :exhale @17:14:46.54 SS.hold @17:14:47.32 PBV.dial between PSV and waste1 @17:14:47.69 PSR.select :air @17:14:47.71 PSV.dial PSR @17:14:48.48 PS.reconfigure :fast @17:14:48.53 PS.seek 2.5ml @17:15:02.96 Intake.select :close @17:15:03.10 SSV.dial :exhaust @17:15:03.84 Exhaust.select :open @17:15:03.95 SS.seek 0ml @17:15:12.40 PSV.dial PTV @17:15:12.98 Exhaust.select :close @17:15:13.09 SSV.dial CBV @17:15:13.11 CBV.dial SSV @17:15:13.18 delay 5 seconds @17:15:13.89 Allowing 130s for 25.0ml of remaining 400.0ml @17:15:13.90 Intake.select :open @17:15:16.06 Intake @16.3psia, vacuum from 10.0 to 13.0psi @17:15:16.07 SS.reconfigure :inhaling 0.34ml/s @17:15:16.12 SS.seek 25ml @17:15:18.20 PSV.dial PSR @17:15:18.96 delay 5 seconds @17:15:23.98 PSV.dial PTV @17:15:24.74 delay 5 seconds @17:15:29.76 PSV.dial :air @17:15:30.51 PS.seek 0ml @17:15:53.84 washCycleDA #2 of 3 @17:15:53.85 PSR.select :air @17:15:53.86 PSV.dial PSR @17:15:55.23 Pulling 0.5ml of PSR.wash @17:15:55.24 PSR.select :wash @17:15:55.35 PS.reconfigure :default @17:15:55.39 PS.seek 0.5ml @17:16:01.30 delay 5 seconds @17:16:06.32 PSR.select :air @17:16:06.43 PS.seek 2ml @17:16:23.02 PTV.dial :puck @17:16:23.03 PSV.dial PTV @17:16:23.77 PBV.dial :waste1 @17:16:24.21 PS.seek 1.5ml @17:16:30.18 delay 3 seconds @17:16:33.19 PBV.dial between PSV and waste1 @17:16:33.58 PS.seek 0ml @17:16:38.32 Stroke completed -- waiting for pressure to equalize ... @17:16:38.34 SS.reconfigure :exhale @17:16:38.38 SS.hold @17:16:49.68 delay 2:00 @17:17:06.86 Intake.select :close @17:17:07.00 SSV.dial :exhaust @17:17:07.76 Exhaust.select :open @17:17:07.87 SS.seek 0ml @17:17:16.68 Exhaust.select :close @17:17:16.79 SSV.dial CBV @17:17:16.81 CBV.dial SSV @17:17:17.59 Allowing 130s for 25.0ml of remaining 375.0ml @17:17:17.60 Intake.select :open @17:17:19.76 Intake @16.2psia, vacuum from 10.0 to 13.0psi @17:17:19.78 SS.reconfigure :inhaling 0.34ml/s @17:17:19.82 SS.seek 25ml @17:18:33.87 Vacuum too high -- waiting up to 40 seconds @17:18:33.89 SS.reconfigure :exhale @17:18:33.93 SS.hold @17:18:49.69 recoverPSRreagent08 @17:18:49.70 PTV.dial :puck @17:18:49.71 PBV.dial :waste1 @17:18:50.09 delay 30 seconds @17:18:50.26 Intake @16.1psia, vacuum from 10.0 to 13.0psi @17:18:50.28 SS.reconfigure :inhaling 0.31ml/s @17:18:50.32 SS.seek 25ml @17:18:58.30 Stroke completed -- waiting for pressure to equalize ... @17:18:58.32 SS.reconfigure :exhale @17:18:58.36 SS.hold @17:19:16.68 Intake.select :close @17:19:16.82 SSV.dial :exhaust @17:19:17.57 Exhaust.select :open @17:19:18.21 SS.seek 0ml @17:19:20.10 PBV.dial between PSV and waste1 @17:19:20.47 PSR.select :air @17:19:20.49 PSV.dial PSR @17:19:21.26 PS.reconfigure :fast @17:19:21.30 PS.seek 2.5ml @17:19:27.34 Exhaust.select :close @17:19:27.44 SSV.dial CBV @17:19:27.46 CBV.dial SSV @17:19:28.23 Allowing 130s for 25.0ml of remaining 350.0ml @17:19:28.24 Intake.select :open @17:19:30.40 Intake @16.2psia, vacuum from 10.0 to 13.0psi @17:19:30.42 SS.reconfigure :inhaling 0.31ml/s @17:19:30.46 SS.seek 25ml @17:19:45.08 PSV.dial PTV @17:19:45.96 delay 5 seconds @17:19:50.98 PSV.dial PSR @17:19:51.71 delay 5 seconds @17:19:56.73 PSV.dial PTV @17:19:57.47 delay 5 seconds @17:20:02.49 PSV.dial :air @17:20:03.26 PS.seek 0ml @17:20:26.36 washCycleDA #3 of 3 @17:20:26.37 PSR.select :air @17:20:26.39 PSV.dial PSR @17:20:27.76 Pulling 0.5ml of PSR.wash @17:20:27.77 PSR.select :wash @17:20:27.88 PS.reconfigure :default @17:20:27.92 PS.seek 0.5ml @17:20:33.86 delay 5 seconds @17:20:38.88 PSR.select :air @17:20:38.99 PS.seek 2ml @17:20:55.17 PTV.dial :puck @17:20:55.18 PSV.dial PTV @17:20:55.91 PBV.dial :waste1 @17:20:56.33 PS.seek 1.5ml @17:21:02.18 Stroke completed -- waiting for pressure to equalize ... @17:21:02.20 SS.reconfigure :exhale @17:21:02.25 SS.hold @17:21:02.27 delay 3 seconds @17:21:05.29 PBV.dial between PSV and waste1 @17:21:05.65 PS.seek 0ml @17:21:21.98 delay 2:00 @17:21:36.97 Intake.select :close @17:21:37.11 SSV.dial :exhaust @17:21:37.87 Exhaust.select :open @17:21:37.97 SS.seek 0ml @17:21:46.88 Exhaust.select :close @17:21:46.99 SSV.dial CBV @17:21:47.02 CBV.dial SSV @17:21:47.79 Allowing 130s for 25.0ml of remaining 325.0ml @17:21:47.80 Intake.select :open @17:21:49.95 Intake @16.4psia, vacuum from 10.0 to 13.0psi @17:21:49.97 SS.reconfigure :inhaling 0.31ml/s @17:21:50.02 SS.seek 25ml @17:22:59.59 Vacuum too high -- waiting up to 40 seconds @17:22:59.60 SS.reconfigure :exhale @17:22:59.64 SS.hold @17:23:16.48 Intake @16.7psia, vacuum from 10.0 to 13.0psi @17:23:16.49 SS.reconfigure :inhaling 0.28ml/s @17:23:16.54 SS.seek 25ml @17:23:22.00 recoverPSRreagent08 @17:23:22.01 PTV.dial :puck @17:23:22.02 PBV.dial :waste1 @17:23:22.37 delay 30 seconds @17:23:42.85 Stroke completed -- waiting for pressure to equalize ... @17:23:42.86 SS.reconfigure :exhale @17:23:42.90 SS.hold @17:23:52.39 PBV.dial between PSV and waste1 @17:23:52.75 PSR.select :air @17:23:52.76 PSV.dial PSR @17:23:53.52 PS.reconfigure :fast @17:23:53.57 PS.seek 2.5ml @17:23:57.81 SS.reconfigure :default @17:23:57.85 SS.hold @17:23:57.97 Intake.select :close @17:23:58.11 ShallowSampler::Clogged in WCR -- Filtering too Slowly after 700.0ml @17:23:58.12 SSV.dial :exhaust @17:23:58.87 Exhaust.select :open @17:23:58.98 SS.reconfigure :exhale @17:23:59.02 SS.seek 0ml @17:24:08.17 Exhaust.select :close @17:24:08.28 Sampled 700.0ml @17:24:08.30 Drying puck before fixing for 20:00 @17:24:08.31 CBV.dial :waste2b @17:24:09.05 delay 1:00 @17:24:17.23 PSV.dial PTV @17:24:17.98 delay 5 seconds @17:24:23.00 PSV.dial PSR @17:24:23.75 delay 5 seconds @17:24:28.77 PSV.dial PTV @17:24:29.51 delay 5 seconds @17:24:34.53 PSV.dial :air @17:24:35.27 PS.seek 0ml @17:24:58.35 Gate.power :camera,:ON @17:24:58.37 Mixing DA Substrates @17:24:58.38 PSR.select :air @17:24:58.39 PSV.dial PSR @17:24:59.92 Pulling 0.25ml of PSR.sub1 @17:24:59.95 PSR.select :sub1 @17:25:00.11 PS.reconfigure :default @17:25:00.24 PS.seek 0.25ml @17:25:03.61 delay 5 seconds @17:25:08.67 Pulling 0.25ml of PSR.sub2 @17:25:08.68 PSR.select :sub2 @17:25:08.80 PS.seek 0.5ml @17:25:09.07 CSV.dial :air @17:25:09.54 CS.reconfigure :fast @17:25:09.59 CS.seek 10ml @17:25:12.17 delay 5 seconds @17:25:17.19 PSR.select :air @17:25:17.29 PS.seek 2ml @17:25:33.38 PTV.dial :puck @17:25:33.40 PSV.dial PTV @17:25:34.26 PBV.dial :waste1 @17:25:34.36 Pumping wcr puck from tube 4 up to 35psi... @17:25:34.38 CBV.dial SSV @17:25:34.69 PS.seek 1.5ml @17:25:35.69 CBV.dial between CSV and waste2b @17:25:36.65 CSV.dial between air and CTV @17:25:36.99 CS.seek 7.143ml @17:25:40.60 delay 3 seconds @17:25:43.62 PBV.dial between PSV and waste1 @17:25:44.00 PS.seek 0ml @17:25:44.46 CTV.dial CSV,avoiding: :air @17:25:44.48 CSV.dial CTV @17:25:45.30 CS.reconfigure :pressurize @17:25:45.35 CS.seek 0ml @17:26:00.10 delay 10 seconds @17:26:01.07 CBV.dial :waste2b @17:26:01.40 CS.reconfigure :default @17:26:01.44 CS.seek 0ml @17:26:05.40 delay 1:00 @17:26:10.13 recoverPSRreagent08 @17:26:10.14 PTV.dial :puck @17:26:10.15 PBV.dial :waste1 @17:26:10.51 delay 30 seconds @17:26:40.53 PBV.dial between PSV and waste1 @17:26:41.40 PSR.select :air @17:26:41.42 PSV.dial PSR @17:26:42.17 PS.reconfigure :fast @17:26:42.22 PS.seek 2.5ml @17:27:05.41 CTV.dial :air,avoiding: :intake @17:27:06.13 PSV.dial PTV @17:27:06.76 Fix #1 of 2 (with RNAlater) for 10:00 @17:27:06.77 CSV.dial CSR @17:27:06.90 delay 5 seconds @17:27:07.26 Pulling 1.999ml of CSR.RNAlater @17:27:07.27 CSR.select :RNAlater @17:27:07.38 CS.seek 2ml @17:27:11.92 PSV.dial PSR @17:27:12.68 delay 5 seconds @17:27:13.30 delay 5 seconds @17:27:17.70 PSV.dial PTV @17:27:18.31 CSR.select :air @17:27:18.42 CS.reconfigure :fast @17:27:18.47 delay 5 seconds @17:27:18.51 CS.seek 10ml @17:27:23.49 PSV.dial :air @17:27:24.27 PS.seek 0ml @17:27:38.33 Pumping wcr puck from tube 4 up to 35psi... @17:27:38.34 CBV.dial SSV @17:27:39.66 CBV.dial between CSV and waste2b @17:27:40.63 CSV.dial between air and CTV @17:27:41.23 CS.seek 7.045ml @17:27:47.69 Additional air clear @17:27:47.70 PSR.select :air @17:27:47.72 PSV.dial PSR @17:27:48.90 CTV.dial CSV,avoiding: :air @17:27:48.92 CSV.dial CTV @17:27:49.09 Pulling 2ml of PSR.air @17:27:49.10 PSR.select :air @17:27:49.13 PS.reconfigure :default @17:27:49.17 PS.seek 2ml @17:27:50.32 CS.reconfigure :pressurize @17:27:50.37 CS.seek 0ml @17:28:08.26 delay 10:00 @17:28:10.35 delay 5 seconds @17:28:15.37 PTV.dial :puck @17:28:15.38 PSV.dial PTV @17:28:16.24 PBV.dial :waste1 @17:28:16.66 PS.seek 1.5ml @17:28:22.66 delay 3 seconds @17:28:25.68 PBV.dial between PSV and waste1 @17:28:26.06 delay 1 second @17:28:27.07 PSV.dial :waste1 @17:28:28.13 PS.seek 0ml @17:28:44.34 Processing Puck Heater at 28C before imaging @17:28:44.35 PH.coast @17:28:44.47 Imaging da puck in Processing Clamp while storing it to tube 3 @17:28:44.63 SC.reconfigure :tight @17:28:44.67 SC.seek 3 @17:28:48.25 SC.configure :limp @17:28:48.45 Elbow.reconfigure :tight @17:28:48.49 Elbow.seek 3 @17:28:50.26 Forearm.reconfigure :tight @17:28:50.31 Forearm.seek 3 + 450 counts @17:28:51.29 SE.reconfigure :pick @17:28:51.34 SE.seek :beamBreak @17:28:54.07 SE.configure :default @17:28:54.21 Elbow.reconfigure :limp @17:28:54.26 Forearm.reconfigure :loose @17:28:54.30 Forearm.seek 3 @17:28:54.54 Elbow.hold @17:28:54.60 Elbow.reconfigure :tight @17:28:54.73 SE.seek 7.13 Pucks below Top Plate @17:28:55.21 Forearm.force -20 @17:28:55.24 Forearm.seek 3 + 305 counts @17:28:55.51 Forearm.reconfigure :tight @17:28:55.56 Forearm.seek 3 + 450 counts @17:28:55.75 SE.seek 8.13 Pucks below Top Plate @17:28:56.26 Forearm.reconfigure :loose @17:28:56.31 Forearm.seek :retract @17:28:57.22 Elbow.seek PC @17:28:59.69 Elbow.reconfigure :loose @17:28:59.78 Forearm.seek PC @17:29:01.24 Hand.close @17:29:01.84 PC.seek :open @17:29:06.61 Forearm.seek :retract @17:29:07.54 Elbow.reconfigure :tight @17:29:07.59 Elbow.seek 3 @17:29:10.20 Forearm.reconfigure :tight @17:29:10.25 Forearm.seek 3 + 450 counts @17:29:11.19 Hand.open @17:29:11.90 SE.seek 7.78 Pucks below Top Plate @17:29:12.16 SE.reconfigure :seal @17:29:12.21 SE.seek 6.81 Pucks below Top Plate @17:29:12.72 Lights, camera, ACTION! @17:29:12.76 PuckCamera: snap -bin=2,2 -auto /var/log/esp/da23feb2817h1000ml.tif @17:29:32.26 Waiting >20 seconds for da to relinquish FlushPuck @17:29:37.63 SX HX9/16 (USB:19): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 21.569 seconds /var/log/esp/da23feb2817h1000ml.tif: TIFF Upload Complete @17:29:37.65 PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da23feb2817h1000ml15s.tif @17:29:53.27 SX HX9/16 (USB:19): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15 seconds /var/log/esp/midres/da23feb2817h1000ml15s.tif: TIFF Upload Complete @17:29:53.29 PuckCamera: snap -bin=2,2 20.0 /var/log/esp/midres/da23feb2817h1000ml20s.tif @17:30:13.95 SX HX9/16 (USB:19): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 20 seconds /var/log/esp/midres/da23feb2817h1000ml20s.tif: TIFF Upload Complete @17:30:13.97 DAfans.email "21.569 sec auto-exposure",Subject: "da23feb2817h1000ml images!" @17:30:13.99 Gate.power :camera,:OFF @17:30:14.34 Elbow.seek 3 @17:30:14.56 Forearm.seek 3 + 450 counts @17:30:14.72 SE.reconfigure :gently @17:30:14.77 SE.seek 7.53 Pucks below Top Plate @17:30:15.40 Elbow.reconfigure :limp @17:30:15.45 Forearm.reconfigure :loose @17:30:15.51 Forearm.seek 3 @17:30:15.80 Elbow.hold @17:30:15.88 Elbow.reconfigure :tight @17:30:15.93 SE.reconfigure :default @17:30:15.98 SE.seek 7.71 Pucks below Top Plate @17:30:16.45 Forearm.force -20 @17:30:16.51 Forearm.seek 3 + 299 counts @17:30:16.81 Forearm.reconfigure :tight @17:30:16.86 Forearm.seek 3 + 450 counts @17:30:17.12 SE.seek :down @17:30:18.26 Forearm.reconfigure :loose @17:30:18.31 Forearm.seek :retract @17:30:19.30 SE.stop @17:30:19.40 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @17:30:19.60 Elbow.seek FlushPuck::Garage @17:30:21.66 Elbow.reconfigure :loose @17:30:21.87 Forearm.seek :garage @17:30:22.89 Hand.close @17:30:23.73 Forearm.seek :retract @17:30:24.81 Elbow.reconfigure :tight @17:30:24.91 Elbow.seek PC @17:30:26.32 Elbow.reconfigure :loose @17:30:26.52 Forearm.reconfigure :tight @17:30:26.60 Forearm.seek PC @17:30:28.15 PC.seek :closed @17:30:32.87 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.60 @17:30:33.02 Hand.open @17:30:34.82 Forearm.reconfigure :loose @17:30:34.92 Forearm.seek :retract @17:30:35.90 PSR.select :air @17:30:35.96 PSV.dial PSR @17:30:36.62 Pulling 0.5ml of PSR.flush @17:30:36.64 PSR.select :flush @17:30:36.80 PS.seek 0.5ml @17:30:42.76 delay 5 seconds @17:30:47.80 PSR.select :air @17:30:47.98 PS.seek 2.5ml @17:30:52.33 upsync @17:31:09.22 delay 1 second @17:31:10.24 PRV.dial :waste2,avoiding: :ab2 @17:31:12.10 PTV.dial :PRVmixing @17:31:13.41 PSV.dial :mixloop @17:31:14.45 PS.seek :empty @17:31:40.81 PSR.select :air @17:31:40.83 PSV.dial PSR @17:31:41.93 Pulling 0.5ml of PSR.flush @17:31:41.94 PSR.select :flush @17:31:42.06 PS.seek 0.5ml @17:31:48.01 delay 5 seconds @17:31:53.02 PSR.select :air @17:31:53.14 PS.seek 2.5ml @17:32:14.38 delay 1 second @17:32:15.39 PBV.dial :waste1 @17:32:15.77 PTV.dial :mixing @17:32:16.53 PSV.dial :mixloop @17:32:17.58 PS.seek :empty @17:32:43.94 PSV.dial PSR @17:32:44.98 PSR.select :flush @17:32:45.10 PS.seek 1ml @17:32:56.15 PSR.select :air @17:32:56.27 PS.seek 2.5ml @17:33:12.40 PSV.dial PTV @17:33:13.28 PTV.dial :puck @17:33:14.02 PBV.dial :waste1 @17:33:14.04 PS.seek 0ml @17:33:40.44 PSV.dial PSR @17:33:41.17 PSR.select :flush @17:33:41.29 PS.seek 0.5ml @17:33:47.22 PSR.select :air @17:33:47.33 PS.seek 2.5ml @17:34:08.78 PSV.dial PTV @17:34:09.53 PBV.dial PSV @17:34:10.10 PS.seek 0ml @17:34:36.62 PSV.dial PBV @17:34:37.10 PS.seek 2.5ml @17:35:03.37 PSV.dial :waste1 @17:35:04.11 PS.seek 0ml @17:35:30.53 PSV.dial PSR @17:35:31.00 PSR.select :air @17:35:31.02 PS.seek 2.5ml @17:35:57.37 PSV.dial PTV @17:35:58.11 PBV.dial PSV @17:35:58.13 PS.seek 0ml @17:36:24.40 PSV.dial PBV @17:36:24.88 PS.seek 2.5ml @17:36:51.28 PSV.dial :waste1 @17:36:52.14 PS.seek 0ml @17:37:18.49 PSV.dial PSR @17:37:18.95 PSR.select :flush @17:37:19.06 PS.seek 1ml @17:37:30.12 PSR.select :air @17:37:30.23 PS.seek 2.5ml @17:37:46.43 PSV.dial PTV @17:37:47.17 PTV.dial :puck @17:37:47.18 PBV.dial :waste1 @17:37:47.74 PS.seek 0ml @17:38:08.28 CBV.dial :waste2b @17:38:08.60 CS.reconfigure :default @17:38:08.65 CS.seek 0ml @17:38:09.94 delay 1:00 @17:38:14.12 PSV.dial PSR @17:38:14.87 PSR.select :flush @17:38:14.98 PS.seek 0.5ml @17:38:20.87 PSR.select :air @17:38:20.98 PS.seek 2.5ml @17:38:42.24 PSV.dial PTV @17:38:42.99 PBV.dial PSV @17:38:43.67 PS.seek 0ml @17:39:09.96 CTV.dial :air,avoiding: :intake @17:39:10.21 PSV.dial PBV @17:39:10.69 PS.seek 2.5ml @17:39:11.29 Fix #2 of 2 (with RNAlater) for 10:00 @17:39:11.30 CSV.dial CSR @17:39:11.90 Pulling 2ml of CSR.RNAlater @17:39:11.91 CSR.select :RNAlater @17:39:12.48 CS.seek 2ml @17:39:18.44 delay 5 seconds @17:39:23.46 CSR.select :air @17:39:23.57 CS.reconfigure :fast @17:39:23.62 CS.seek 10ml @17:39:37.12 PSV.dial :waste1 @17:39:37.87 PS.seek 0ml @17:39:43.29 Pumping wcr puck from tube 4 up to 35psi... @17:39:43.30 CBV.dial SSV @17:39:44.60 CBV.dial between CSV and waste2b @17:39:45.55 CSV.dial between air and CTV @17:39:46.14 CS.seek 7.025ml @17:39:53.77 CTV.dial CSV,avoiding: :air @17:39:53.79 CSV.dial CTV @17:39:55.18 CS.reconfigure :pressurize @17:39:55.23 CS.seek 0ml @17:40:04.27 PSV.dial PSR @17:40:04.74 PSR.select :air @17:40:04.77 PS.seek 2.5ml @17:40:09.43 delay 10:00 @17:40:31.08 PSV.dial PTV @17:40:31.94 PBV.dial PSV @17:40:31.96 PS.seek 0ml @17:40:58.36 PSV.dial PBV @17:40:58.83 PS.seek 2.5ml @17:41:25.21 PSV.dial :waste1 @17:41:25.97 PS.seek 0ml @17:41:52.52 Garaging FlushPuck in Processing Clamp @17:41:52.59 Elbow.reconfigure :tight @17:41:52.64 Elbow.seek PC @17:41:52.74 Elbow.reconfigure :loose @17:41:52.83 Forearm.seek PC @17:41:53.81 Hand.close @17:41:54.45 PC.seek :open @17:41:59.19 Forearm.seek :retract @17:42:00.14 Elbow.reconfigure :tight @17:42:00.19 Elbow.seek :garage @17:42:01.55 Elbow.reconfigure :loose @17:42:01.64 Forearm.reconfigure :tight @17:42:01.69 Forearm.seek :garage @17:42:02.67 Hand.open @17:42:03.38 Forearm.reconfigure :loose @17:42:03.43 Forearm.seek :retract @17:50:09.45 CBV.dial :waste2b @17:50:09.78 CS.reconfigure :default @17:50:09.82 CS.seek 0ml @17:50:14.75 delay 1:00 @17:51:14.77 CTV.dial :air,avoiding: :intake @17:51:16.14 Storing wcr puck in Collection Clamp to tube 3 @17:51:16.50 Elbow.reconfigure :tight @17:51:16.55 Elbow.seek 3 @17:51:18.38 Forearm.reconfigure :tight @17:51:18.43 Forearm.seek 3 + 450 counts @17:51:19.49 SE.reconfigure :pick @17:51:19.54 SE.seek :beamBreak @17:51:22.08 SE.configure :default @17:51:22.23 Elbow.reconfigure :limp @17:51:22.28 Forearm.reconfigure :loose @17:51:22.32 Forearm.seek 3 @17:51:22.58 Elbow.hold @17:51:22.64 Elbow.reconfigure :tight @17:51:22.73 SE.seek 8.12 Pucks below Top Plate @17:51:23.19 Forearm.force -20 @17:51:23.22 Forearm.seek 3 + 284 counts @17:51:23.51 Forearm.reconfigure :tight @17:51:23.56 Forearm.seek 3 + 450 counts @17:51:24.32 SE.seek 9.12 Pucks below Top Plate @17:51:24.86 Forearm.reconfigure :loose @17:51:24.90 Forearm.seek :retract @17:51:25.81 Elbow.seek CC @17:51:27.46 Elbow.reconfigure :loose @17:51:27.55 Forearm.seek CC @17:51:28.54 Hand.close @17:51:29.20 CC.seek :open @17:51:33.93 Forearm.seek :retract @17:51:34.91 Elbow.reconfigure :tight @17:51:34.96 Elbow.seek 3 @17:51:36.74 Elbow.reconfigure :limp @17:51:36.79 Forearm.seek 3 @17:51:37.80 Elbow.hold @17:51:37.87 Elbow.reconfigure :tight @17:51:37.91 Hand.open @17:51:38.68 Forearm.force -20 @17:51:38.72 Forearm.seek 3 + 319 counts @17:51:38.99 Forearm.reconfigure :tight @17:51:39.04 Forearm.seek 3 + 450 counts @17:51:39.31 SE.seek :down @17:51:40.43 Forearm.reconfigure :loose @17:51:40.48 Forearm.seek :retract @17:51:41.42 SE.stop @17:51:41.45
Starting finalPurge @17:51:41.47 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @17:51:41.64 Elbow.seek FlushPuck::Garage @17:51:43.40 Elbow.reconfigure :loose @17:51:43.50 Forearm.seek :garage @17:51:44.46 Hand.close @17:51:45.18 Forearm.seek :retract @17:51:46.18 Elbow.reconfigure :tight @17:51:46.22 Elbow.seek CC @17:51:49.17 Elbow.reconfigure :loose @17:51:49.26 Forearm.reconfigure :tight @17:51:49.30 Forearm.seek CC @17:51:50.52 CC.seek :closed @17:51:55.04 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.30 @17:51:55.11 Hand.open @17:51:55.75 Forearm.reconfigure :loose @17:51:55.79 Forearm.seek :retract @17:51:56.74 Flushing the Collection Valve::Manifold @17:51:56.86 CSV.dial CSR @17:51:57.45 Pulling 5ml of CSR.flush @17:51:57.46 CSR.select :flush @17:51:57.57 CS.seek 5ml @17:52:11.15 delay 5 seconds @17:52:16.16 CSR.select :air @17:52:16.32 CS.reconfigure :fast @17:52:16.36 CS.seek 7.501ml @17:52:23.05 CSV.dial :waste @17:52:24.31 CS.reconfigure :default @17:52:24.35 CS.seek 0ml @17:52:44.30 Flushing lower manifold's waste1 port @17:52:44.41 CSV.dial :flush @17:52:44.87 CS.reconfigure :fast @17:52:44.91 CS.seek 5ml @17:52:57.71 delay 5 seconds @17:53:02.72 CSV.dial :air @17:53:03.22 CS.seek 7ml @17:53:08.83 CSV.dial CTV @17:53:08.86
CTV.dial CSV @17:53:10.68 CBV.dial :waste1 @17:53:11.43 CS.seek 0ml @17:53:27.90 Flushing lower manifold's waste2 port @17:53:28.01 CSV.dial :flush @17:53:28.73 CS.seek 5ml @17:53:41.30 delay 5 seconds @17:53:46.32 CSV.dial :air @17:53:46.83 CS.seek 7ml @17:53:52.27 CSV.dial CTV @17:53:52.29
CTV.dial CSV @17:53:52.74 CBV.dial :waste2 @17:53:53.79 CS.seek 0ml @17:54:10.30 Flushing lower manifold's waste2b port @17:54:10.41 CSV.dial :flush @17:54:11.15 CS.seek 5ml @17:54:23.67 delay 5 seconds @17:54:28.69 CSV.dial :air @17:54:29.20 CS.seek 7ml @17:54:34.64 CSV.dial CTV @17:54:34.67
CTV.dial CSV @17:54:35.11 CBV.dial :waste2b @17:54:36.15 CS.seek 0ml @17:54:52.64 Disinfecting external Intake with kill @17:54:52.76 CSV.dial CSR @17:54:53.24 Pulling 8ml of CSR.kill @17:54:53.25 CSR.select :kill @17:54:53.36 CS.seek 8ml @17:55:12.96 delay 5 seconds @17:55:17.97 CSR.select :air @17:55:18.09 CS.seek 10ml @17:55:23.61 CSV.dial CBV @17:55:23.63
CBV.dial CSV @17:55:24.67 CTV.dial :intake @17:55:25.45 Intake.select :open @17:55:25.56 CS.seek 2ml @17:55:44.30 Intake.select :close @17:55:44.40 CTV.dial :air @17:55:45.16 CS.reconfigure :default @17:55:45.20 CS.seek 0ml @17:55:51.17 CSV.dial CSR @17:55:52.21 Pulling 4.999ml of CSR.flush @17:55:52.22 CSR.select :flush @17:55:52.34 CS.seek 5ml @17:56:06.14 delay 5 seconds @17:56:11.16 CSR.select :air @17:56:11.27 CS.seek 10ml @17:56:24.86 CSV.dial CBV @17:56:25.85 CS.seek 0ml @17:56:52.33 Garaging FlushPuck in Collection Clamp @17:56:52.40 Elbow.reconfigure :tight @17:56:52.44 Elbow.seek CC @17:56:52.88 Elbow.reconfigure :loose @17:56:52.97 Forearm.seek CC @17:56:53.90 Hand.close @17:56:54.57 CC.seek :open @17:56:59.18 Forearm.seek :retract @17:57:00.14 Elbow.reconfigure :tight @17:57:00.18 Elbow.seek :garage @17:57:02.96 Elbow.reconfigure :loose @17:57:03.05 Forearm.reconfigure :tight @17:57:03.09 Forearm.seek :garage @17:57:04.10 Hand.open @17:57:04.75 Forearm.reconfigure :loose @17:57:04.79 Forearm.seek :retract @17:57:05.74 Completed [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] begun 15:11:25 @17:57:05.78 Asleep after completing DA_WCR_022823 assays @17:57:05.80 GoodNews.email "{1 => 33,\n 2 => 33,\n 3 => 10,\n 4 => 23,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@17:50:36, 17% flow, 20.4C, 36% humidity, 14.8psia, 13.739V, 0.411A, 0.399A avg, 5.65W\nSampled 700.0ml",Subject: "Asleep after completing DA_WCR_022823 assays" @17:57:11.53 upsync @14:33:18.41UTC01-Mar-23
ESP2 v3.41 I2C Gateway v3.20 for core bus @14:33:20.96 Configuration for short puck jake 1/11/21 by brent@mbari.org /home/esp/esp2/type/shallow/jake/configure.rb @14:33:24.89 Polling Can every 10:00 @14:33:25.11 Cleanly exited at 14:30:50UTC01-Mar-23 @14:33:25.14 Loading /home/esp/esp2/mission/DA_WCR_030123.rb mission @14:33:25.20 Preparing to sleep until + 00:00:05 @14:33:25.21 GoodNews.email "Starting DA_WCR_030123 w/pucks in tube 4\n{1 => 33,\n 2 => 33,\n 3 => 10,\n 4 => 23,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@14:33:24, 15% flow, 20.0C, 37% humidity, 14.8psia, 13.773V, 0.260A, 0.236A avg, 3.58W",Subject: "DA_WCR_030123 begun" @14:33:25.25
Asleep til + 00:00:05 @14:33:25.29 Continuing at 14:33 @14:33:25.53 Gate.power :core,:OFF @14:33:25.55 Gate.power :raw,:OFF @14:33:25.56 Shutdown @14:33:30.16
Powering core bus @14:33:31.67 GoodNews.email "Then, in tube 4: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2/mission/DA_WCR_030123.rb:14\n{1 => 33,\n 2 => 33,\n 3 => 10,\n 4 => 23,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@14:33:25, 15% flow, 20.1C, 37% humidity, 14.8psia, 13.773V, 0.254A, 0.242A avg, 3.50W",Subject: "Asleep until 14:33" @14:33:33.55 upsync @14:33:35.40
Gate.power :raw,:ON @14:33:36.04 CS.configure :default @14:33:36.13 PS.configure :default @14:33:36.22 SS.configure :default @14:33:36.31 CC.configure :default @14:33:36.40 PC.configure :default @14:33:36.51 Forearm.configure :loose @14:33:36.60 Elbow.configure :loose @14:33:36.72 SC.configure :loose @14:33:36.87 SE.configure :default @14:33:36.97 CH.coast @14:33:37.07 CH.configure :default @14:33:37.13 PH.coast @14:33:37.24 PH.configure :default @14:33:37.31 All dwarf controllers running firmware version 3.85 @14:33:37.41 Valve.reset @14:33:37.70 Intake.select :close @14:33:37.72 Exhaust.select :close @14:33:37.73 CTV.dial :air @14:33:37.85 PSV.dial :air @14:33:37.87 CSV.dial :waste1 @14:33:37.97
Forearm.reconfigure :loose @14:33:38.02 Forearm.home.seek :retract @14:33:38.34 SSV.dial :spit,avoiding: :exhaust @14:33:38.75 Hand.close @14:33:38.78
Elbow.jog 1500 @14:33:38.87 upsync @14:33:39.06 PS.reconfigure :default @14:33:39.11 PS.home.seek 0ml @14:33:39.17 CS.reconfigure :fast @14:33:39.20 SS.reconfigure :default @14:33:39.28 CS.home.seek 0ml @14:33:39.31 SS.home.seek 0ml @14:33:39.41
Elbow.reconfigure :loose @14:33:39.45 Elbow.home.seek :clear @14:33:43.91 SE.reconfigure :default @14:33:43.95 SE.home.seek :down @14:33:44.55 SC.reconfigure :tight @14:33:44.60 SC.home.seek :initial @14:33:47.44 PS.stop @14:34:12.99
SC.configure :limp @14:34:13.03 Forearm.seek :retract @14:34:13.09 Elbow.seek :hammer @14:34:14.12 Forearm.reconfigure :tight @14:34:14.16 Forearm.seek retracted @14:34:14.39 PC.reconfigure :default @14:34:14.41
CC.reconfigure :default @14:34:14.50 CC.home.seek :open @14:34:14.51 PC.home.seek :open @14:34:18.02
Phase [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] @14:34:18.03 Notice.email "At 14:34:17 began in tube 4: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2/mission/DA_WCR_030123.rb:14\n{1 => 33,\n 2 => 33,\n 3 => 10,\n 4 => 23,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@14:34:17, 16% flow, 20.0C, 37% humidity, 14.8psia, 13.754V, 0.321A, 0.302A avg, 4.41W",Subject: "Started DA_WCR_030123 phase initialPurge; da [1000, 1000] #da and wsr are run simultanelously" @14:34:18.05
Starting initialPurge @14:34:18.08 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:34:18.14 Hand.open @14:34:18.87 Elbow.reconfigure :tight @14:34:18.91 Elbow.seek FlushPuck::Garage @14:34:20.80 Elbow.reconfigure :loose @14:34:20.89 Forearm.reconfigure :loose @14:34:20.93 Forearm.seek :garage @14:34:21.89 Hand.close @14:34:22.61 Forearm.seek :retract @14:34:23.58 Elbow.reconfigure :tight @14:34:23.63 Elbow.seek CC @14:34:24.47 upsync @14:34:26.57
Elbow.reconfigure :loose @14:34:26.66 Forearm.reconfigure :tight @14:34:26.71 Forearm.seek CC @14:34:27.95 CC.seek :closed @14:34:32.50 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.35 @14:34:32.58 Hand.open @14:34:33.29 Forearm.reconfigure :loose @14:34:33.33 Forearm.seek :retract @14:34:34.28 Flushing the Collection Valve::Manifold @14:34:34.39 CSV.dial CSR @14:34:35.72 Pulling 5ml of CSR.flush @14:34:35.73 CSR.select :flush @14:34:35.84 CS.reconfigure :default @14:34:35.88 CS.seek 5ml @14:34:49.71 delay 5 seconds @14:34:54.73 CSR.select :air @14:34:54.88 CS.reconfigure :fast @14:34:54.92 CS.seek 7.5ml @14:35:01.64 CSV.dial :waste @14:35:02.90 CS.reconfigure :default @14:35:02.94 CS.seek 0ml @14:35:22.87 Purging external Intake @14:35:22.98 CSV.dial :flush @14:35:23.42 CS.reconfigure :fast @14:35:23.46 CS.seek 10ml @14:35:47.80 delay 5 seconds @14:35:52.82 CSV.dial CBV @14:35:52.85
CBV.dial CSV @14:35:53.60 CTV.dial :intake @14:35:54.38 Intake.select :open @14:35:54.48 CS.seek 0ml @14:36:17.99 Intake.select :close @14:36:18.08 CTV.dial :air @14:36:18.87 Flushing upper manifold @14:36:18.98 CSV.dial :flush @14:36:19.71 CS.seek 5ml @14:36:32.49 delay 5 seconds @14:36:37.51 CSV.dial :air @14:36:38.00 CS.seek 7ml @14:36:43.49 CSV.dial CBV @14:36:43.52
CBV.dial CSV @14:36:44.53 CTV.dial :air @14:36:44.55 CS.seek 0ml @14:37:01.05 delay 7 seconds @14:37:08.08 CS.seek 3ml @14:37:16.05 CSV.dial :waste @14:37:16.49 CS.seek 0ml @14:37:24.24 CSV.dial :flush @14:37:24.68 CS.seek 3ml @14:37:32.74 delay 5 seconds @14:37:37.75 CSV.dial :air @14:37:38.25 CS.seek 5ml @14:37:43.70 CSV.dial :air @14:37:43.72 CS.seek 0ml @14:37:55.80 delay 7 seconds @14:38:02.82 CS.seek 3ml @14:38:10.80 CSV.dial :waste @14:38:11.52 CS.seek 0ml @14:38:19.14 Garaging FlushPuck in Collection Clamp @14:38:19.21 Elbow.reconfigure :tight @14:38:19.25 Elbow.seek CC @14:38:19.53 Elbow.reconfigure :loose @14:38:19.61 Forearm.seek CC @14:38:20.58 Hand.close @14:38:21.23 CC.seek :open @14:38:25.88 Forearm.seek :retract @14:38:26.85 Elbow.reconfigure :tight @14:38:26.90 Elbow.seek :garage @14:38:29.81 Elbow.reconfigure :loose @14:38:29.90 Forearm.reconfigure :tight @14:38:29.94 Forearm.seek :garage @14:38:30.97 Hand.open @14:38:32.03 Forearm.reconfigure :loose @14:38:32.07 Forearm.seek :retract @14:38:33.02 da sampling at most 1000ml, wcr at most 1000ml @14:38:33.06 Loading sh1 puck from tube 4 into Collection Clamp @14:38:33.07 DAfans.email "Started da in tube 4 at 14:38:33UTC01-Mar-23\n{1 => 33,\n 2 => 33,\n 3 => 10,\n 4 => 23,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@14:34:17, 16% flow, 20.0C, 37% humidity, 14.8psia, 13.754V, 0.321A, 0.302A avg, 4.41W",Subject: "da sampling at most 1000ml, wcr at most 1000ml" @14:38:33.55
Elbow.reconfigure :tight @14:38:33.62 Elbow.seek 4 @14:38:35.57 Forearm.reconfigure :tight @14:38:35.62 Forearm.seek 4 + 450 counts @14:38:36.66 SE.reconfigure :pick @14:38:36.73 SE.seek :beamBreak @14:38:38.34 SE.configure :default @14:38:38.50 Elbow.seek 4 @14:38:38.69 Forearm.seek 4 + 300 counts @14:38:38.71 upsync @14:38:38.91
Forearm.reconfigure :loose @14:38:38.96 Hand.close @14:38:39.62 SE.seek :down @14:38:39.82
Forearm.seek :retract @14:38:40.76 Elbow.seek CC @14:38:40.91 SE.stop @14:38:42.33
Elbow.reconfigure :loose @14:38:42.42 Forearm.reconfigure :tight @14:38:42.46 Forearm.seek CC @14:38:43.75 CC.seek :closed @14:38:48.31 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.23 @14:38:48.32 Hand.open @14:38:49.03 Forearm.reconfigure :loose @14:38:49.07 Forearm.seek :retract @14:38:50.12 collectSample 1000ml w/bubble point of 24psi @14:38:50.13 Priming 7ml @14:38:50.14 Intake.select :close @14:38:50.15 Exhaust.select :close @14:38:50.16 SS.hold @14:38:50.36 Calibrating pressure and temperature sensors @14:38:50.38 CTV.dial :air @14:38:50.40 SSV.dial :spit,avoiding: :exhaust @14:38:50.41 SS.reconfigure :fast @14:38:50.46 SS.seek 5ml @14:38:53.30 SSV.dial CBV @14:38:53.34
CBV.dial SSV @14:38:54.62 delay 3.8 seconds @14:38:58.54 SSV.dial :spit @14:38:59.83 SS.seek 0ml @14:39:02.61 SSV.dial CBV @14:39:03.91 CTV.dial :intake @14:39:04.69 SSV.dial CBV @14:39:04.71
CBV.dial SSV @14:39:04.73 Allowing 40s for 7.0ml of remaining 7.0ml @14:39:04.74 Intake.select :open @14:39:06.89 Intake @15.2psia, vacuum from 9.7 to 12.7psi @14:39:06.91 SS.reconfigure :inhaling 2.20ml/s @14:39:06.95 SS.seek 7ml @14:39:13.82 Stroke completed -- waiting for pressure to equalize ... @14:39:13.83 SS.reconfigure :exhale @14:39:13.87 SS.hold @14:39:15.81 Intake.select :close @14:39:15.95 SSV.dial :exhaust @14:39:16.70 Exhaust.select :open @14:39:16.81 SS.seek 0ml @14:39:20.89 Exhaust.select :close @14:39:20.99 Sampling 1000.0ml @14:39:21.00 Intake.select :close @14:39:21.01 Exhaust.select :close @14:39:21.02 SS.reconfigure :default @14:39:21.06 SS.hold @14:39:21.24 CTV.dial :intake @14:39:21.25 SSV.dial CBV @14:39:21.27
CBV.dial SSV @14:39:22.05 Allowing 130s for 25.0ml of remaining 1000.0ml @14:39:22.06 Intake.select :open @14:39:24.20 Intake @15.2psia, vacuum from 9.7 to 12.7psi @14:39:24.22 SS.reconfigure :inhaling 2.20ml/s @14:39:24.26 SS.seek 25ml @14:39:35.90 Vacuum too high -- waiting up to 40 seconds SS.reconfigure :exhale @14:39:35.95 SS.hold @14:39:36.93 Intake @12.5psia, vacuum from 7.0 to 10.0psi @14:39:36.95 SS.reconfigure :inhaling 1.98ml/s @14:39:36.99 SS.seek 25ml @14:39:41.82 Stroke completed -- waiting for pressure to equalize ... @14:39:41.83 SS.reconfigure :exhale @14:39:42.27 SS.hold @14:39:47.22 Intake.select :close @14:39:47.35 SSV.dial :exhaust @14:39:48.09 Exhaust.select :open @14:39:48.20 SS.seek 0ml @14:39:57.21 Exhaust.select :close @14:39:57.32 SSV.dial CBV @14:39:57.35
CBV.dial SSV @14:39:58.08 Allowing 130s for 25.0ml of remaining 975.0ml @14:39:58.09 Intake.select :open @14:40:00.24 Intake @15.7psia, vacuum from 10.0 to 13.0psi @14:40:00.25 SS.reconfigure :inhaling 1.98ml/s @14:40:00.30 SS.seek 25ml @14:40:11.58 Vacuum too high -- waiting up to 40 seconds @14:40:11.59 SS.reconfigure :exhale @14:40:11.63 SS.hold @14:40:12.54 Intake @12.8psia, vacuum from 7.3 to 10.3psi @14:40:12.55 SS.reconfigure :inhaling 1.79ml/s @14:40:12.59 SS.seek 25ml @14:40:17.96 Stroke completed -- waiting for pressure to equalize ... @14:40:17.97 SS.reconfigure :exhale @14:40:18.01 SS.hold @14:40:24.40 Intake.select :close @14:40:24.54 SSV.dial :exhaust @14:40:25.29 Exhaust.select :open @14:40:25.40 SS.seek 0ml @14:40:34.17 Exhaust.select :close @14:40:34.28 SSV.dial CBV @14:40:34.31
CBV.dial SSV @14:40:35.06 Allowing 130s for 25.0ml of remaining 950.0ml @14:40:35.07 Intake.select :open @14:40:37.22 Intake @15.7psia, vacuum from 10.0 to 13.0psi @14:40:37.24 SS.reconfigure :inhaling 1.79ml/s @14:40:37.28 SS.seek 25ml @14:40:50.27 Vacuum too high -- waiting up to 40 seconds @14:40:50.28 SS.reconfigure :exhale @14:40:50.32 SS.hold @14:40:50.91 Intake @13.0psia, vacuum from 7.5 to 10.5psi @14:40:50.92 SS.reconfigure :inhaling 1.61ml/s @14:40:50.97 SS.seek 25ml @14:40:56.57 Stroke completed -- waiting for pressure to equalize ... @14:40:56.58 SS.reconfigure :exhale @14:40:56.62 SS.hold @14:41:03.12 Intake.select :close @14:41:03.26 SSV.dial :exhaust @14:41:04.01 Exhaust.select :open @14:41:04.12 SS.seek 0ml @14:41:12.85 Exhaust.select :close @14:41:12.96 SSV.dial CBV @14:41:13.00
CBV.dial SSV @14:41:13.73 Allowing 130s for 25.0ml of remaining 925.0ml @14:41:13.74 Intake.select :open @14:41:15.89 Intake @15.7psia, vacuum from 10.0 to 13.0psi @14:41:15.91 SS.reconfigure :inhaling 1.61ml/s @14:41:15.95 SS.seek 25ml @14:41:34.30 Vacuum too high -- waiting up to 40 seconds @14:41:34.31 SS.reconfigure :exhale @14:41:34.35 SS.hold @14:41:36.01 Intake @13.2psia, vacuum from 7.7 to 10.7psi @14:41:36.02 SS.reconfigure :inhaling 1.45ml/s @14:41:36.06 SS.seek 25ml @14:41:37.10 Stroke completed -- waiting for pressure to equalize ... @14:41:37.11 SS.reconfigure :exhale @14:41:37.15 SS.hold @14:41:42.70 Intake.select :close @14:41:42.83 SSV.dial :exhaust @14:41:43.59 Exhaust.select :open @14:41:43.69 SS.seek 0ml @14:41:52.38 Exhaust.select :close @14:41:52.49 SSV.dial CBV @14:41:52.54
CBV.dial SSV @14:41:53.26 Allowing 130s for 25.0ml of remaining 900.0ml @14:41:53.27 Intake.select :open @14:41:55.42 Intake @15.7psia, vacuum from 10.0 to 13.0psi @14:41:55.44 SS.reconfigure :inhaling 1.45ml/s @14:41:55.48 SS.seek 25ml @14:42:15.84 Stroke completed -- waiting for pressure to equalize ... SS.reconfigure :exhale @14:42:15.88 SS.hold @14:42:22.76 Intake.select :close @14:42:22.90 SSV.dial :exhaust @14:42:23.67 Exhaust.select :open @14:42:23.77 SS.seek 0ml @14:42:32.55 Exhaust.select :close @14:42:32.66 SSV.dial CBV @14:42:32.68
CBV.dial SSV @14:42:33.45 Allowing 130s for 25.0ml of remaining 875.0ml @14:42:33.46 Intake.select :open @14:42:35.61 Intake @15.6psia, vacuum from 10.0 to 13.0psi @14:42:35.62 SS.reconfigure :inhaling 1.45ml/s @14:42:35.67 SS.seek 25ml @14:42:56.16 Stroke completed -- waiting for pressure to equalize ... @14:42:56.17 SS.reconfigure :exhale @14:42:56.21 SS.hold @14:43:03.58 Intake.select :close @14:43:03.71 SSV.dial :exhaust @14:43:04.48 Exhaust.select :open @14:43:04.58 SS.seek 0ml @14:43:13.24 Exhaust.select :close @14:43:13.34 SSV.dial CBV @14:43:13.37
CBV.dial SSV @14:43:14.13 Allowing 130s for 25.0ml of remaining 850.0ml @14:43:14.14 Intake.select :open @14:43:16.30 Intake @15.7psia, vacuum from 10.0 to 13.0psi @14:43:16.31 SS.reconfigure :inhaling 1.45ml/s @14:43:16.35 SS.seek 25ml @14:43:36.74 Stroke completed -- waiting for pressure to equalize ... @14:43:36.75 SS.reconfigure :exhale @14:43:36.79 SS.hold @14:43:45.24 Intake.select :close @14:43:45.38 SSV.dial :exhaust @14:43:46.13 Exhaust.select :open @14:43:46.24 SS.seek 0ml @14:43:55.30 Exhaust.select :close @14:43:55.40 SSV.dial CBV @14:43:55.43
CBV.dial SSV @14:43:56.20 Allowing 130s for 25.0ml of remaining 825.0ml @14:43:56.21 Intake.select :open @14:43:58.36 Intake @15.6psia, vacuum from 10.0 to 13.0psi @14:43:58.37 SS.reconfigure :inhaling 1.45ml/s @14:43:58.41 SS.seek 25ml @14:44:18.35 Stroke completed -- waiting for pressure to equalize ... @14:44:18.36 SS.reconfigure :exhale @14:44:18.40 SS.hold @14:44:26.84 Intake.select :close @14:44:26.97 SSV.dial :exhaust @14:44:27.72 Exhaust.select :open @14:44:27.83 SS.seek 0ml @14:44:36.61 Exhaust.select :close @14:44:36.72 SSV.dial CBV @14:44:36.77
CBV.dial SSV @14:44:37.49 Allowing 130s for 25.0ml of remaining 800.0ml @14:44:37.50 Intake.select :open @14:44:39.66 Intake @15.7psia, vacuum from 10.0 to 13.0psi @14:44:39.67 SS.reconfigure :inhaling 1.45ml/s @14:44:39.71 SS.seek 25ml @14:44:57.35 Vacuum too high -- waiting up to 40 seconds @14:44:57.36 SS.reconfigure :exhale @14:44:57.41 SS.hold @14:44:59.53 Intake @13.4psia, vacuum from 7.9 to 10.9psi @14:44:59.55 SS.reconfigure :inhaling 1.30ml/s @14:44:59.59 SS.seek 25ml @14:45:03.23 Stroke completed -- waiting for pressure to equalize ... @14:45:03.24 SS.reconfigure :exhale @14:45:03.28 SS.hold @14:45:09.75 Intake.select :close @14:45:09.89 SSV.dial :exhaust @14:45:10.65 Exhaust.select :open @14:45:11.24 SS.seek 0ml @14:45:20.00 Exhaust.select :close @14:45:20.11 SSV.dial CBV @14:45:20.14
CBV.dial SSV @14:45:20.88 Allowing 130s for 25.0ml of remaining 775.0ml @14:45:20.89 Intake.select :open @14:45:23.04 Intake @15.6psia, vacuum from 10.0 to 13.0psi @14:45:23.06 SS.reconfigure :inhaling 1.30ml/s @14:45:23.10 SS.seek 25ml @14:45:45.33 Stroke completed -- waiting for pressure to equalize ... @14:45:45.34 SS.reconfigure :exhale @14:45:45.38 SS.hold @14:45:53.82 Intake.select :close @14:45:53.96 SSV.dial :exhaust @14:45:54.69 Exhaust.select :open @14:45:54.80 SS.seek 0ml @14:46:03.63 Exhaust.select :close @14:46:03.73 SSV.dial CBV @14:46:03.75
CBV.dial SSV @14:46:04.51 Allowing 130s for 25.0ml of remaining 750.0ml @14:46:04.52 Intake.select :open @14:46:06.67 Intake @15.6psia, vacuum from 10.0 to 13.0psi @14:46:06.68 SS.reconfigure :inhaling 1.30ml/s @14:46:06.72 SS.seek 25ml @14:46:28.86 Stroke completed -- waiting for pressure to equalize ... @14:46:28.87 SS.reconfigure :exhale @14:46:28.91 SS.hold @14:46:37.37 Intake.select :close @14:46:37.51 SSV.dial :exhaust @14:46:38.25 Exhaust.select :open @14:46:38.36 SS.seek 0ml @14:46:47.35 Exhaust.select :close @14:46:47.45 SSV.dial CBV @14:46:47.47
CBV.dial SSV @14:46:48.22 Allowing 130s for 25.0ml of remaining 725.0ml @14:46:48.23 Intake.select :open @14:46:50.39 Intake @15.6psia, vacuum from 10.0 to 13.0psi @14:46:50.40 SS.reconfigure :inhaling 1.30ml/s @14:46:50.45 SS.seek 25ml @14:47:12.22 Stroke completed -- waiting for pressure to equalize ... @14:47:12.23 SS.reconfigure :exhale @14:47:12.27 SS.hold @14:47:22.02 Intake.select :close @14:47:22.16 SSV.dial :exhaust @14:47:22.91 Exhaust.select :open @14:47:23.02 SS.seek 0ml @14:47:31.86 Exhaust.select :close @14:47:31.96 SSV.dial CBV @14:47:31.99
CBV.dial SSV @14:47:32.75 Allowing 130s for 25.0ml of remaining 700.0ml @14:47:32.76 Intake.select :open @14:47:34.92 Intake @15.6psia, vacuum from 10.0 to 13.0psi @14:47:34.93 SS.reconfigure :inhaling 1.30ml/s @14:47:34.97 SS.seek 25ml @14:47:56.64 Vacuum too high -- waiting up to 40 seconds @14:47:56.65 SS.reconfigure :exhale @14:47:56.69 SS.hold @14:47:59.70 Intake @13.2psia, vacuum from 7.7 to 10.7psi @14:47:59.72 SS.reconfigure :inhaling 1.17ml/s @14:47:59.76 SS.seek 25ml @14:47:59.98 Stroke completed -- waiting for pressure to equalize ... @14:47:59.99 SS.reconfigure :exhale @14:48:00.03 SS.hold @14:48:06.42 Intake.select :close @14:48:06.56 SSV.dial :exhaust @14:48:07.31 Exhaust.select :open @14:48:07.41 SS.seek 0ml @14:48:16.16 Exhaust.select :close @14:48:16.26 SSV.dial CBV @14:48:16.28
CBV.dial SSV @14:48:17.03 Allowing 130s for 25.0ml of remaining 675.0ml @14:48:17.04 Intake.select :open @14:48:19.19 Intake @15.6psia, vacuum from 10.0 to 13.0psi @14:48:19.21 SS.reconfigure :inhaling 1.17ml/s @14:48:19.25 SS.seek 25ml @14:48:43.98 Stroke completed -- waiting for pressure to equalize ... @14:48:43.99 SS.reconfigure :exhale @14:48:44.03 SS.hold @14:48:52.56 Intake.select :close @14:48:52.70 SSV.dial :exhaust @14:48:53.45 Exhaust.select :open @14:48:53.56 SS.seek 0ml @14:49:02.70 Exhaust.select :close @14:49:02.81 SSV.dial CBV @14:49:02.83
CBV.dial SSV @14:49:03.60 Allowing 130s for 25.0ml of remaining 650.0ml @14:49:03.61 Intake.select :open @14:49:05.76 Intake @15.6psia, vacuum from 10.0 to 13.0psi @14:49:05.78 SS.reconfigure :inhaling 1.17ml/s @14:49:05.82 SS.seek 25ml @14:49:29.47 Stroke completed -- waiting for pressure to equalize ... @14:49:29.48 SS.reconfigure :exhale @14:49:29.52 SS.hold @14:49:39.17 Intake.select :close @14:49:39.31 SSV.dial :exhaust @14:49:40.07 Exhaust.select :open @14:49:40.17 SS.seek 0ml @14:49:49.22 Exhaust.select :close @14:49:49.32 SSV.dial CBV @14:49:49.34
CBV.dial SSV @14:49:50.11 Allowing 130s for 25.0ml of remaining 625.0ml @14:49:50.12 Intake.select :open @14:49:52.27 Intake @15.6psia, vacuum from 10.0 to 13.0psi @14:49:52.29 SS.reconfigure :inhaling 1.17ml/s @14:49:52.33 SS.seek 25ml @14:50:15.87 Stroke completed -- waiting for pressure to equalize ... @14:50:15.88 SS.reconfigure :exhale @14:50:15.92 SS.hold @14:50:25.57 Intake.select :close @14:50:25.71 SSV.dial :exhaust @14:50:26.47 Exhaust.select :open @14:50:26.57 SS.seek 0ml @14:50:35.61 Exhaust.select :close @14:50:35.71 SSV.dial CBV @14:50:35.73
CBV.dial SSV @14:50:36.50 Allowing 130s for 25.0ml of remaining 600.0ml @14:50:36.51 Intake.select :open @14:50:38.66 Intake @15.7psia, vacuum from 10.0 to 13.0psi @14:50:38.68 SS.reconfigure :inhaling 1.17ml/s @14:50:38.72 SS.seek 25ml @14:50:59.44 Vacuum too high -- waiting up to 40 seconds @14:50:59.45 SS.reconfigure :exhale @14:50:59.49 SS.hold @14:51:03.68 Intake @13.8psia, vacuum from 8.3 to 11.3psi @14:51:03.70 SS.reconfigure :inhaling 1.06ml/s @14:51:03.74 SS.seek 25ml @14:51:07.65 Stroke completed -- waiting for pressure to equalize ... @14:51:07.66 SS.reconfigure :exhale @14:51:07.70 SS.hold @14:51:14.11 Intake.select :close @14:51:14.24 SSV.dial :exhaust @14:51:15.00 Exhaust.select :open @14:51:15.10 SS.seek 0ml @14:51:23.92 Exhaust.select :close @14:51:24.02 SSV.dial CBV @14:51:24.04
CBV.dial SSV @14:51:24.81 Allowing 130s for 25.0ml of remaining 575.0ml @14:51:25.29 Intake.select :open @14:51:27.46 Intake @15.6psia, vacuum from 10.0 to 13.0psi @14:51:27.48 SS.reconfigure :inhaling 1.06ml/s @14:51:27.52 SS.seek 25ml @14:51:53.87 Stroke completed -- waiting for pressure to equalize ... @14:51:53.88 SS.reconfigure :exhale @14:51:53.92 SS.hold @14:52:03.67 Intake.select :close @14:52:03.81 SSV.dial :exhaust @14:52:04.56 Exhaust.select :open @14:52:04.67 SS.seek 0ml @14:52:13.56 Exhaust.select :close @14:52:13.66 SSV.dial CBV @14:52:13.68
CBV.dial SSV @14:52:14.44 Allowing 130s for 25.0ml of remaining 550.0ml @14:52:14.45 Intake.select :open @14:52:16.60 Intake @15.6psia, vacuum from 10.0 to 13.0psi @14:52:16.61 SS.reconfigure :inhaling 1.06ml/s @14:52:16.66 SS.seek 25ml @14:52:43.01 Stroke completed -- waiting for pressure to equalize ... @14:52:43.02 SS.reconfigure :exhale @14:52:43.06 SS.hold @14:52:54.08 Intake.select :close @14:52:54.21 SSV.dial :exhaust @14:52:54.95 Exhaust.select :open @14:52:55.06 SS.seek 0ml @14:53:04.07 Exhaust.select :close @14:53:04.18 SSV.dial CBV @14:53:04.23
CBV.dial SSV @14:53:04.93 Allowing 130s for 25.0ml of remaining 525.0ml @14:53:04.94 Intake.select :open @14:53:07.10 Intake @15.6psia, vacuum from 10.0 to 13.0psi @14:53:07.12 SS.reconfigure :inhaling 1.06ml/s @14:53:07.16 SS.seek 25ml @14:53:32.56 Vacuum too high -- waiting up to 40 seconds @14:53:32.57 SS.reconfigure :exhale @14:53:32.61 SS.hold @14:53:38.23 Intake @14.4psia, vacuum from 8.9 to 11.9psi @14:53:38.24 SS.reconfigure :inhaling 0.95ml/s @14:53:38.28 SS.seek 25ml @14:53:39.35 Stroke completed -- waiting for pressure to equalize ... @14:53:39.36 SS.reconfigure :exhale @14:53:39.40 SS.hold @14:53:44.94 Intake.select :close @14:53:45.08 SSV.dial :exhaust @14:53:45.84 Exhaust.select :open @14:53:45.95 SS.seek 0ml @14:53:54.77 Exhaust.select :close @14:53:54.88 SSV.dial CBV @14:53:54.90
CBV.dial SSV @14:53:55.67 Allowing 130s for 25.0ml of remaining 500.0ml @14:53:55.68 Intake.select :open @14:53:57.83 Intake @15.7psia, vacuum from 10.0 to 13.0psi @14:53:57.84 SS.reconfigure :inhaling 0.95ml/s @14:53:57.89 SS.seek 25ml @14:54:26.54 Stroke completed -- waiting for pressure to equalize ... @14:54:26.55 SS.reconfigure :exhale @14:54:26.59 SS.hold @14:54:37.78 Intake.select :close @14:54:37.91 SSV.dial :exhaust @14:54:38.65 Exhaust.select :open @14:54:38.76 SS.seek 0ml @14:54:47.56 Exhaust.select :close @14:54:47.66 SSV.dial CBV @14:54:47.68
CBV.dial SSV @14:54:48.43 Allowing 130s for 25.0ml of remaining 475.0ml @14:54:48.44 Intake.select :open @14:54:50.60 Intake @15.5psia, vacuum from 10.0 to 13.0psi @14:54:50.61 SS.reconfigure :inhaling 0.95ml/s @14:54:50.66 SS.seek 25ml @14:55:19.68 Stroke completed -- waiting for pressure to equalize ... @14:55:19.69 SS.reconfigure :exhale @14:55:19.73 SS.hold @14:55:32.26 Intake.select :close @14:55:32.40 SSV.dial :exhaust @14:55:33.15 Exhaust.select :open @14:55:33.26 SS.seek 0ml @14:55:42.07 Exhaust.select :close @14:55:42.17 SSV.dial CBV @14:55:42.19
CBV.dial SSV @14:55:42.95 Allowing 130s for 25.0ml of remaining 450.0ml @14:55:42.96 Intake.select :open @14:55:45.11 Intake @15.6psia, vacuum from 10.0 to 13.0psi @14:55:45.12 SS.reconfigure :inhaling 0.95ml/s @14:55:45.17 SS.seek 25ml @14:56:13.38 Vacuum too high -- waiting up to 40 seconds @14:56:13.39 SS.reconfigure :exhale @14:56:13.43 SS.hold @14:56:19.06 Intake @14.4psia, vacuum from 8.9 to 11.9psi @14:56:19.07 SS.reconfigure :inhaling 0.87ml/s @14:56:19.12 SS.seek 25ml @14:56:20.30 Stroke completed -- waiting for pressure to equalize ... @14:56:20.31 SS.reconfigure :exhale @14:56:20.35 SS.hold @14:56:26.77 Intake.select :close @14:56:26.91 SSV.dial :exhaust @14:56:27.65 Exhaust.select :open @14:56:27.76 SS.seek 0ml @14:56:36.60 Exhaust.select :close @14:56:36.70 SSV.dial CBV @14:56:36.72
CBV.dial SSV @14:56:37.48 Allowing 130s for 25.0ml of remaining 425.0ml @14:56:37.49 Intake.select :open @14:56:39.64 Intake @15.6psia, vacuum from 10.0 to 13.0psi @14:56:39.65 SS.reconfigure :inhaling 0.87ml/s @14:56:39.70 SS.seek 25ml @14:57:11.24 Stroke completed -- waiting for pressure to equalize ... @14:57:11.25 SS.reconfigure :exhale @14:57:11.29 SS.hold @14:57:23.88 Intake.select :close @14:57:24.02 SSV.dial :exhaust @14:57:24.76 Exhaust.select :open @14:57:24.86 SS.seek 0ml @14:57:33.69 Exhaust.select :close @14:57:33.80 SSV.dial CBV @14:57:33.85
CBV.dial SSV @14:57:34.55 Allowing 130s for 25.0ml of remaining 400.0ml @14:57:34.56 Intake.select :open @14:57:36.72 Intake @15.5psia, vacuum from 10.0 to 13.0psi @14:57:36.74 SS.reconfigure :inhaling 0.87ml/s @14:57:36.78 SS.seek 25ml @14:58:07.90 Vacuum too high -- waiting up to 40 seconds @14:58:07.91 SS.reconfigure :exhale @14:58:07.95 SS.hold @14:58:14.45 Intake @14.7psia, vacuum from 9.2 to 12.2psi @14:58:14.47 SS.reconfigure :inhaling 0.78ml/s @14:58:14.51 SS.seek 25ml @14:58:15.85 Stroke completed -- waiting for pressure to equalize ... @14:58:15.86 SS.reconfigure :exhale @14:58:15.90 SS.hold @14:58:21.56 Intake.select :close @14:58:21.70 SSV.dial :exhaust @14:58:22.44 Exhaust.select :open @14:58:22.55 SS.seek 0ml @14:58:31.74 Exhaust.select :close @14:58:31.84 SSV.dial CBV @14:58:31.86
CBV.dial SSV @14:58:32.62 Allowing 130s for 25.0ml of remaining 375.0ml @14:58:32.63 Intake.select :open @14:58:34.78 Intake @15.6psia, vacuum from 10.0 to 13.0psi @14:58:34.80 SS.reconfigure :inhaling 0.78ml/s @14:58:34.84 SS.seek 25ml @14:59:08.91 Stroke completed -- waiting for pressure to equalize ... @14:59:08.92 SS.reconfigure :exhale @14:59:08.96 SS.hold @14:59:23.30 Intake.select :close @14:59:23.44 SSV.dial :exhaust @14:59:24.18 Exhaust.select :open @14:59:24.28 SS.seek 0ml @14:59:33.36 Exhaust.select :close @14:59:33.47 SSV.dial CBV @14:59:33.49
CBV.dial SSV @14:59:34.24 Allowing 130s for 25.0ml of remaining 350.0ml @14:59:34.25 Intake.select :open @14:59:36.40 Intake @15.6psia, vacuum from 10.0 to 13.0psi @14:59:36.42 SS.reconfigure :inhaling 0.78ml/s @14:59:36.46 SS.seek 25ml @15:00:10.58 Stroke completed -- waiting for pressure to equalize ... @15:00:10.59 SS.reconfigure :exhale @15:00:10.63 SS.hold @15:00:25.34 Intake.select :close @15:00:25.47 SSV.dial :exhaust @15:00:26.22 Exhaust.select :open @15:00:26.33 SS.seek 0ml @15:00:35.19 Exhaust.select :close @15:00:35.29 SSV.dial CBV @15:00:35.31
CBV.dial SSV @15:00:36.07 Allowing 130s for 25.0ml of remaining 325.0ml @15:00:36.08 Intake.select :open @15:00:38.23 Intake @15.5psia, vacuum from 10.0 to 13.0psi @15:00:38.25 SS.reconfigure :inhaling 0.78ml/s @15:00:38.29 SS.seek 25ml @15:01:13.08 Stroke completed -- waiting for pressure to equalize ... @15:01:13.09 SS.reconfigure :exhale @15:01:13.13 SS.hold @15:01:29.38 Intake.select :close @15:01:29.52 SSV.dial :exhaust @15:01:30.27 Exhaust.select :open @15:01:30.37 SS.seek 0ml @15:01:39.50 Exhaust.select :close @15:01:39.60 SSV.dial CBV @15:01:39.62
CBV.dial SSV @15:01:40.38 Allowing 130s for 25.0ml of remaining 300.0ml @15:01:40.39 Intake.select :open @15:01:42.54 Intake @15.5psia, vacuum from 10.0 to 13.0psi @15:01:42.56 SS.reconfigure :inhaling 0.78ml/s @15:01:42.60 SS.seek 25ml @15:02:16.62 Vacuum too high -- waiting up to 40 seconds @15:02:16.63 SS.reconfigure :exhale @15:02:16.67 SS.hold @15:02:25.24 Intake @14.7psia, vacuum from 9.2 to 12.2psi @15:02:25.25 SS.reconfigure :inhaling 0.70ml/s @15:02:25.30 SS.seek 25ml @15:02:25.50 Stroke completed -- waiting for pressure to equalize ... @15:02:25.51 SS.reconfigure :exhale @15:02:25.55 SS.hold @15:02:33.99 Intake.select :close @15:02:34.12 SSV.dial :exhaust @15:02:34.86 Exhaust.select :open @15:02:34.97 SS.seek 0ml @15:02:44.03 Exhaust.select :close @15:02:44.13 SSV.dial CBV @15:02:44.15
CBV.dial SSV @15:02:44.91 Allowing 130s for 25.0ml of remaining 275.0ml @15:02:44.92 Intake.select :open @15:02:47.07 Intake @15.5psia, vacuum from 10.0 to 13.0psi @15:02:47.08 SS.reconfigure :inhaling 0.70ml/s @15:02:47.13 SS.seek 25ml @15:03:24.78 Stroke completed -- waiting for pressure to equalize ... @15:03:24.79 SS.reconfigure :exhale @15:03:24.83 SS.hold @15:03:41.01 Intake.select :close @15:03:41.15 SSV.dial :exhaust @15:03:41.89 Exhaust.select :open @15:03:42.00 SS.seek 0ml @15:03:50.86 Exhaust.select :close @15:03:50.96 SSV.dial CBV @15:03:50.99
CBV.dial SSV @15:03:51.73 Allowing 130s for 25.0ml of remaining 250.0ml @15:03:51.74 Intake.select :open @15:03:53.90 Intake @15.4psia, vacuum from 9.9 to 12.9psi @15:03:53.92 SS.reconfigure :inhaling 0.70ml/s @15:03:53.96 SS.seek 25ml @15:04:26.43 Vacuum too high -- waiting up to 40 seconds @15:04:26.44 SS.reconfigure :exhale @15:04:26.48 SS.hold @15:04:35.02 Intake @14.6psia, vacuum from 9.1 to 12.1psi @15:04:35.04 SS.reconfigure :inhaling 0.63ml/s @15:04:35.08 SS.seek 25ml @15:04:41.70 Stroke completed -- waiting for pressure to equalize ... @15:04:41.71 SS.reconfigure :exhale @15:04:41.75 SS.hold @15:04:51.41 Intake.select :close @15:04:51.55 SSV.dial :exhaust @15:04:52.30 Exhaust.select :open @15:04:52.41 SS.seek 0ml @15:05:01.23 Exhaust.select :close @15:05:01.33 SSV.dial CBV @15:05:01.35
CBV.dial SSV @15:05:02.11 Allowing 130s for 25.0ml of remaining 225.0ml @15:05:02.12 Intake.select :open @15:05:04.27 Intake @15.4psia, vacuum from 9.9 to 12.9psi @15:05:04.29 SS.reconfigure :inhaling 0.63ml/s @15:05:04.33 SS.seek 25ml @15:05:44.43 Vacuum too high -- waiting up to 40 seconds @15:05:44.44 SS.reconfigure :exhale @15:05:44.48 SS.hold @15:05:53.01 Intake @14.7psia, vacuum from 9.2 to 12.2psi @15:05:53.03 SS.reconfigure :inhaling 0.57ml/s @15:05:53.07 SS.seek 25ml @15:05:55.96 Stroke completed -- waiting for pressure to equalize ... @15:05:55.97 SS.reconfigure :exhale @15:05:56.02 SS.hold @15:06:05.78 Intake.select :close @15:06:05.91 SSV.dial :exhaust @15:06:06.66 Exhaust.select :open @15:06:06.76 SS.seek 0ml @15:06:15.82 Exhaust.select :close @15:06:15.92 SSV.dial CBV @15:06:15.97
CBV.dial SSV @15:06:16.70 Allowing 130s for 25.0ml of remaining 200.0ml @15:06:16.71 Intake.select :open @15:06:18.86 Intake @15.5psia, vacuum from 10.0 to 13.0psi @15:06:18.88 SS.reconfigure :inhaling 0.57ml/s @15:06:18.92 SS.seek 25ml @15:07:04.82 Stroke completed -- waiting for pressure to equalize ... @15:07:04.83 SS.reconfigure :exhale @15:07:05.34 SS.hold @15:07:23.47 Intake.select :close @15:07:23.60 SSV.dial :exhaust @15:07:24.35 Exhaust.select :open @15:07:24.46 SS.seek 0ml @15:07:33.32 Exhaust.select :close @15:07:33.42 SSV.dial CBV @15:07:33.44
CBV.dial SSV @15:07:34.20 Allowing 130s for 25.0ml of remaining 175.0ml @15:07:34.21 Intake.select :open @15:07:36.36 Intake @15.5psia, vacuum from 10.0 to 13.0psi @15:07:36.38 SS.reconfigure :inhaling 0.57ml/s @15:07:36.42 SS.seek 25ml @15:08:21.83 Vacuum too high -- waiting up to 40 seconds @15:08:21.84 SS.reconfigure :exhale @15:08:21.88 SS.hold @15:08:32.99 Intake @15.4psia, vacuum from 9.9 to 12.9psi @15:08:33.01 SS.reconfigure :inhaling 0.51ml/s @15:08:33.06 SS.seek 25ml @15:08:34.40 Stroke completed -- waiting for pressure to equalize ... @15:08:34.41 SS.reconfigure :exhale @15:08:34.45 SS.hold @15:08:44.13 Intake.select :close @15:08:44.26 SSV.dial :exhaust @15:08:45.01 Exhaust.select :open @15:08:45.11 SS.seek 0ml @15:08:54.18 Exhaust.select :close @15:08:54.28 SSV.dial CBV @15:08:54.30
CBV.dial SSV @15:08:55.06 Allowing 130s for 25.0ml of remaining 150.0ml @15:08:55.07 Intake.select :open @15:08:57.23 Intake @15.6psia, vacuum from 10.0 to 13.0psi @15:08:57.24 SS.reconfigure :inhaling 0.51ml/s @15:08:57.29 SS.seek 25ml @15:09:48.05 Stroke completed -- waiting for pressure to equalize ... @15:09:48.06 SS.reconfigure :exhale @15:09:48.10 SS.hold @15:10:08.44 Intake.select :close @15:10:08.58 SSV.dial :exhaust @15:10:09.32 Exhaust.select :open @15:10:09.43 SS.seek 0ml @15:10:18.54 Exhaust.select :close @15:10:19.11 SSV.dial CBV @15:10:19.15
CBV.dial SSV @15:10:19.88 Allowing 130s for 25.0ml of remaining 125.0ml @15:10:19.89 Intake.select :open @15:10:22.05 Intake @15.5psia, vacuum from 10.0 to 13.0psi @15:10:22.06 SS.reconfigure :inhaling 0.51ml/s @15:10:22.11 SS.seek 25ml @15:11:13.39 Stroke completed -- waiting for pressure to equalize ... @15:11:13.40 SS.reconfigure :exhale @15:11:13.45 SS.hold @15:11:35.74 Intake.select :close @15:11:35.87 SSV.dial :exhaust @15:11:36.61 Exhaust.select :open @15:11:36.72 SS.seek 0ml @15:11:45.77 Exhaust.select :close @15:11:45.87 SSV.dial CBV @15:11:45.89
CBV.dial SSV @15:11:46.65 Allowing 130s for 25.0ml of remaining 100.0ml @15:11:46.66 Intake.select :open @15:11:48.82 Intake @15.5psia, vacuum from 10.0 to 13.0psi @15:11:48.83 SS.reconfigure :inhaling 0.51ml/s @15:11:48.87 SS.seek 25ml @15:12:24.45 Vacuum too high -- waiting up to 40 seconds @15:12:24.46 SS.reconfigure :exhale @15:12:24.50 SS.hold @15:12:34.15 Intake @15.2psia, vacuum from 9.7 to 12.7psi @15:12:34.16 SS.reconfigure :inhaling 0.47ml/s @15:12:34.21 SS.seek 25ml @15:12:51.81 Stroke completed -- waiting for pressure to equalize ... @15:12:51.82 SS.reconfigure :exhale @15:12:51.86 SS.hold @15:13:10.12 Intake.select :close @15:13:10.26 SSV.dial :exhaust @15:13:11.00 Exhaust.select :open @15:13:11.11 SS.seek 0ml @15:13:20.13 Exhaust.select :close @15:13:20.23 SSV.dial CBV @15:13:20.25
CBV.dial SSV @15:13:21.00 Allowing 130s for 25.0ml of remaining 75.0ml @15:13:21.01 Intake.select :open @15:13:23.16 Intake @15.5psia, vacuum from 10.0 to 13.0psi @15:13:23.18 SS.reconfigure :inhaling 0.47ml/s @15:13:23.23 SS.seek 25ml @15:14:19.53 Stroke completed -- waiting for pressure to equalize ... @15:14:19.54 SS.reconfigure :exhale @15:14:19.58 SS.hold @15:14:45.96 Intake.select :close @15:14:46.10 SSV.dial :exhaust @15:14:46.85 Exhaust.select :open @15:14:46.96 SS.seek 0ml @15:14:55.81 Exhaust.select :close @15:14:55.91 SSV.dial CBV @15:14:55.94
CBV.dial SSV @15:14:56.69 Allowing 130s for 25.0ml of remaining 50.0ml @15:14:56.70 Intake.select :open @15:14:58.85 Intake @15.6psia, vacuum from 10.0 to 13.0psi @15:14:58.87 SS.reconfigure :inhaling 0.47ml/s @15:14:58.91 SS.seek 25ml @15:15:40.38 Vacuum too high -- waiting up to 40 seconds @15:15:40.39 SS.reconfigure :exhale @15:15:40.43 SS.hold @15:15:52.97 Intake @15.3psia, vacuum from 9.8 to 12.8psi @15:15:52.98 SS.reconfigure :inhaling 0.43ml/s @15:15:53.03 SS.seek 25ml @15:16:10.62 Stroke completed -- waiting for pressure to equalize ... @15:16:10.63 SS.reconfigure :exhale @15:16:10.67 SS.hold @15:16:30.94 Intake.select :close @15:16:31.08 SSV.dial :exhaust @15:16:31.83 Exhaust.select :open @15:16:31.94 SS.seek 0ml @15:16:40.81 Exhaust.select :close @15:16:40.91 SSV.dial CBV @15:16:40.93
CBV.dial SSV @15:16:41.69 Allowing 130s for 25.0ml of remaining 25.0ml @15:16:41.70 Intake.select :open @15:16:43.85 Intake @15.6psia, vacuum from 10.0 to 13.0psi @15:16:43.86 SS.reconfigure :inhaling 0.43ml/s @15:16:43.91 SS.seek 24.95ml @15:17:40.16 Vacuum too high -- waiting up to 40 seconds @15:17:40.17 SS.reconfigure :exhale @15:17:40.21 SS.hold @15:17:52.74 Intake @15.3psia, vacuum from 9.8 to 12.8psi @15:17:52.75 SS.reconfigure :inhaling 0.38ml/s @15:17:52.80 SS.seek 24.95ml @15:18:03.03 Stroke completed -- waiting for pressure to equalize ... SS.reconfigure :exhale @15:18:03.07 SS.hold @15:18:21.57 Intake.select :close @15:18:21.71 SSV.dial :exhaust @15:18:22.45 Exhaust.select :open @15:18:22.56 SS.seek 0ml @15:18:31.40 Exhaust.select :close @15:18:31.50 Sampled 1000.0ml @15:18:31.52 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) Intake.select :close @15:18:31.58 CSV.dial :waste @15:18:31.65 CS.seek 0ml @15:18:31.72 SSV.dial CBV,avoiding: :exhaust @15:18:31.74
CBV.dial SSV @15:18:31.81 CSV.dial :air @15:18:32.55 CS.seek 10ml @15:18:33.61
CTV.dial :air @15:18:34.40 delay 15 seconds @15:18:49.42 SSV.dial :spit,avoiding: :exhaust @15:18:50.73 CTV.dial CSV,avoiding: :intake @15:18:50.77
SS.seek 0ml @15:18:50.88 SSV.dial CBV,avoiding: :exhaust @15:18:56.94 CBV.dial between SSV and waste2 @15:18:57.32 CTV.dial CSV,avoiding: :air @15:18:57.34
CSV.dial CTV @15:18:57.78 CS.reconfigure :pressurize @15:18:57.82 CS.seek 0ml @15:19:16.82 BubblePoint: Intake @39.3psia, Outlet @14.9psia @15:19:16.83 CBV.dial SSV @15:19:17.15 SS.reconfigure :default @15:19:17.19 SS.seek 10ml @15:19:22.89 delay 15 seconds @15:19:38.00 Evacuated: Intake @16.7psia, Outlet @10.2psia @15:19:38.01 SSV.dial :spit,avoiding: :exhaust @15:19:39.33 SS.reconfigure :exhale @15:19:39.37 SS.seek 0ml @15:19:44.83 SS.reconfigure :fast @15:19:44.87 SS.seek 5ml @15:19:47.80 SSV.dial CBV,avoiding: :exhaust @15:19:47.83
CBV.dial SSV @15:19:49.12 CTV.dial :air,avoiding: :intake @15:19:50.46 CSV.dial CTV @15:19:50.50
SS.seek 10ml @15:19:51.49 CSV.dial :air @15:19:51.95 CS.reconfigure :fast @15:19:51.99 CS.seek 0ml @15:19:53.12
SSV.dial :spit,avoiding: :exhaust @15:19:54.44 SS.reconfigure :exhale @15:19:54.49 SS.seek 0ml @15:19:59.19 CS.stop @15:19:59.82
SS.reconfigure :fast @15:19:59.86 SS.seek 5ml @15:20:02.72 SSV.dial CBV,avoiding: :exhaust @15:20:02.74
CBV.dial SSV @15:20:05.07 SSV.dial :spit,avoiding: :exhaust @15:20:06.39 SS.reconfigure :default @15:20:06.43 SS.seek 0ml @15:20:09.74 SS.stop @15:20:09.78 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @15:20:09.79 Intake.select :close @15:20:09.86 CSV.dial :waste @15:20:09.97 SSV.dial CBV,avoiding: :exhaust @15:20:09.99
CBV.dial SSV @15:20:10.61 CS.seek 0ml @15:20:10.67 CSV.dial :air @15:20:11.31
CTV.dial :air @15:20:11.32 delay 15 seconds @15:20:11.39 CS.seek 10ml @15:20:26.34
SSV.dial :spit,avoiding: :exhaust @15:20:27.66 CTV.dial CSV,avoiding: :intake @15:20:27.68
SS.reconfigure :exhale @15:20:27.77 SS.seek 0ml @15:20:28.17 SSV.dial CBV,avoiding: :exhaust @15:20:35.94 CBV.dial between SSV and waste2 @15:20:36.32 CTV.dial CSV,avoiding: :air @15:20:36.34
CSV.dial CTV @15:20:36.78 CS.reconfigure :pressurize @15:20:36.82 CS.seek 0ml @15:20:58.13 BubblePoint: Intake @38.3psia, Outlet @13.9psia @15:20:58.14 CBV.dial SSV @15:20:58.90 SS.reconfigure :default @15:20:58.94 SS.seek 10ml @15:21:04.54 delay 15 seconds @15:21:19.66 Evacuated: Intake @18.0psia, Outlet @11.6psia @15:21:19.67 SSV.dial :spit,avoiding: :exhaust @15:21:20.98 SS.reconfigure :exhale @15:21:21.03 SS.seek 0ml @15:21:26.38 SS.reconfigure :fast @15:21:26.42 SS.seek 5ml @15:21:29.30 SSV.dial CBV,avoiding: :exhaust @15:21:29.32
CBV.dial SSV @15:21:30.62 CTV.dial :air,avoiding: :intake @15:21:31.94 CSV.dial CTV @15:21:31.98
SS.seek 10ml @15:21:32.97 CSV.dial :air @15:21:33.43 CS.reconfigure :fast @15:21:33.47 CS.seek 0ml @15:21:35.40
SSV.dial :spit,avoiding: :exhaust @15:21:36.72 SS.reconfigure :exhale @15:21:36.77 SS.seek 0ml @15:21:38.63 CS.stop @15:21:42.17
SS.reconfigure :fast @15:21:42.22 SS.seek 5ml @15:21:45.08 SSV.dial CBV,avoiding: :exhaust @15:21:45.11
CBV.dial SSV @15:21:47.39 SSV.dial :spit,avoiding: :exhaust @15:21:48.72 SS.reconfigure :default @15:21:48.76 SS.seek 0ml @15:21:52.13 SS.stop @15:21:52.17 Lysing: Adding 2.0ml AqMeOH @15:21:52.18 CBV.dial between CSV and waste1,avoiding: :DBV @15:21:53.66 CTV.dial CSV @15:21:54.97 CSV.dial CSR @15:21:55.87 Pulling 1ml of CSR.AqMeOH @15:21:55.88 CSR.select :AqMeOH @15:21:55.98 CS.reconfigure :default @15:21:56.02 CS.seek 1ml @15:21:59.69 delay 5 seconds @15:22:04.71 CSR.select :air @15:22:04.87 CS.seek 6ml @15:22:18.63 delay 2 seconds @15:22:20.64 CSV.dial :waste2 @15:22:21.10 CS.reconfigure :fast @15:22:21.14 CS.seek 0ml @15:22:35.44 CSV.dial CSR @15:22:35.93 Pulling 2ml of CSR.AqMeOH @15:22:35.94 CSR.select :AqMeOH @15:22:36.05 CS.reconfigure :default @15:22:36.09 CS.seek 2ml @15:22:42.38 delay 5 seconds @15:22:47.40 CSR.select :air @15:22:47.56 CS.seek 5ml @15:22:56.22 delay 2 seconds @15:22:58.24 CSV.dial CTV @15:22:58.66 CS.seek 1ml @15:23:09.82 Before lysing Collection Puck Heater at 19.8C/38.3psia @15:23:09.84 Heating Puck.from 4, :sh1 in Collection Clamp to 70C for 10:00 @15:23:09.86 CH.seek 70C,8:00 @15:25:06.53 CH.hold 10:00,70C @15:25:06.71 Begun lysing Collection Puck Heater at 68C/46.7psia @15:35:06.72 Ended lysing Collection Puck Heater at 70C/45.2psia @15:35:06.73 CS.seek 0ml @15:35:10.09 CTV.dial between spare and air,avoiding: :intake @15:35:11.12 CSV.dial :air @15:35:11.56 CS.seek 5ml @15:35:25.36 CSV.dial CTV @15:35:25.80 delay 2 seconds @15:35:27.82 CSV.dial :air @15:35:28.27 delay 2 seconds @15:35:30.29 CSV.dial CTV @15:35:30.74 delay 2 seconds @15:35:32.76 CSV.dial :air @15:35:33.21 delay 2 seconds @15:35:35.23 CS.seek 0ml @15:35:48.98 Puck @72.2psia after final lysis pressurization @15:35:48.99 Recovering Lysate into Collection syringe @15:35:49.00 CSV.dial CBV @15:35:49.99 CBV.dial CSV @15:35:50.36 CS.seek 5ml @15:36:04.18 delay 5 seconds @15:36:09.19 CTV.dial :air @15:36:09.70 CS.seek 7ml @15:36:15.68 delay 5 seconds @15:36:20.69 CSV.dial :air2 @15:36:21.13 CS.seek 10ml @15:36:29.61 CSV.dial between air and CTV,avoiding: CBV @15:36:30.76 Lysing Complete @15:36:30.83 Storing sh1 puck in Collection Clamp to tube 3 @15:36:30.99 SC.reconfigure :tight @15:36:31.03 SC.seek 3 @15:36:34.63 SC.configure :limp @15:36:34.81 Elbow.reconfigure :tight @15:36:34.86 Elbow.seek 3 @15:36:36.63 Forearm.reconfigure :tight @15:36:36.67 Forearm.seek 3 + 450 counts @15:36:37.66 SE.reconfigure :pick @15:36:37.70 SE.seek :beamBreak @15:36:40.28 SE.configure :default @15:36:40.43 Elbow.reconfigure :limp @15:36:40.48 Forearm.reconfigure :loose @15:36:40.52 Forearm.seek 3 @15:36:40.74 Elbow.hold @15:36:40.80 Elbow.reconfigure :tight @15:36:40.88 SE.seek 9.13 Pucks below Top Plate @15:36:41.26 Forearm.force -20 @15:36:41.29 Forearm.seek 3 + 317 counts @15:36:41.57 Forearm.reconfigure :tight @15:36:41.61 Forearm.seek 3 + 450 counts @15:36:41.82 SE.seek 10.13 Pucks below Top Plate @15:36:42.31 Forearm.reconfigure :loose @15:36:42.36 Forearm.seek :retract @15:36:43.29 Elbow.seek CC @15:36:44.90 Elbow.reconfigure :loose @15:36:44.99 Forearm.seek CC @15:36:45.95 Hand.close @15:36:46.55 CC.seek :open @15:36:51.26 Forearm.seek :retract @15:36:52.22 Elbow.reconfigure :tight @15:36:52.27 Elbow.seek 3 @15:36:54.05 Elbow.reconfigure :limp @15:36:54.09 Forearm.seek 3 @15:36:55.11 Elbow.hold @15:36:55.17 Elbow.reconfigure :tight @15:36:55.21 Hand.open @15:36:55.91 Forearm.force -20 @15:36:55.94 Forearm.seek 3 + 308 counts @15:36:56.22 Forearm.reconfigure :tight @15:36:56.26 Forearm.seek 3 + 450 counts @15:36:56.97 SE.seek :down @15:36:58.09
Forearm.reconfigure :loose @15:36:58.13 Forearm.seek :retract @15:36:58.98 SE.stop @15:36:59.08
Tranferring DA @15:36:59.10 Beginning samplePassC @15:36:59.11 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @15:36:59.28 Elbow.seek FlushPuck::Garage @15:37:01.17 Elbow.reconfigure :loose @15:37:01.27 Forearm.seek :garage @15:37:02.24 Hand.close @15:37:02.91 Forearm.seek :retract @15:37:03.90 Elbow.reconfigure :tight @15:37:03.94 Elbow.seek CC @15:37:06.90 Elbow.reconfigure :loose @15:37:06.99 Forearm.reconfigure :tight @15:37:07.03 Forearm.seek CC @15:37:08.23 CC.seek :closed @15:37:12.74 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.32 @15:37:12.81 Hand.open @15:37:13.48 Forearm.reconfigure :loose @15:37:13.53 Forearm.seek :retract @15:37:14.45 PSV.dial PSR @15:37:15.81 Pulling 1.5ml of PSR.flush @15:37:15.82 PSR.select :flush @15:37:15.94 PS.seek 1.5ml @15:37:32.12 delay 5 seconds @15:37:37.14 PSV.dial :waste1 @15:37:37.61 PS.seek 0ml @15:37:53.80 PSV.dial PSR @15:37:54.26 PSR.select :air @15:37:54.37 PS.seek 2.5ml @15:38:20.78 PRV.dial CBV,avoiding: :ab2 @15:38:20.80
CBV.dial PRV @15:38:22.46 PSV.dial :mixloop @15:38:23.49 CTV.dial :air,avoiding: :intake @15:38:23.51 PTV.dial :PRVmixing @15:38:24.81 PS.seek 0ml @15:38:51.15 PSV.dial :air @15:38:51.59 PS.seek 2.5ml @15:39:17.93 PSV.dial :mixloop @15:39:18.39 PS.seek 0ml @15:39:44.77 PSV.dial :air @15:39:45.23 PS.seek 2.5ml @15:40:11.62 PSV.dial :mixloop @15:40:12.09 PS.seek 0ml @15:40:38.40 Garaging FlushPuck in Collection Clamp @15:40:38.46 Elbow.reconfigure :tight @15:40:38.51 Elbow.seek CC @15:40:38.88 Elbow.reconfigure :loose @15:40:38.97 Forearm.seek CC @15:40:39.98 Hand.close @15:40:40.55 CC.seek :open @15:40:45.18 Forearm.seek :retract @15:40:46.16 Elbow.reconfigure :tight @15:40:46.20 Elbow.seek :garage @15:40:49.08 Elbow.reconfigure :loose @15:40:49.17 Forearm.reconfigure :tight @15:40:49.22 Forearm.seek :garage @15:40:50.22 Hand.open @15:40:50.85 Forearm.reconfigure :loose @15:40:50.89 Forearm.seek :retract @15:40:51.86 PSV.dial :mixloop @15:40:51.87 PTV.dial :PRVmixing @15:40:51.88 PRV.dial CBV @15:40:51.91
CBV.dial PRV @15:40:51.92 CTV.dial :air,avoiding: :intake @15:40:51.94 Loading lyfil puck from tube 4 into Collection Clamp @15:40:52.62 SC.reconfigure :tight @15:40:52.67 SC.seek 4 @15:40:56.21 SC.configure :limp @15:40:56.40 Elbow.reconfigure :tight @15:40:56.44 Elbow.seek 4 @15:40:58.38 Forearm.reconfigure :tight @15:40:58.42 Forearm.seek 4 + 450 counts @15:40:59.50 SE.reconfigure :pick @15:40:59.55 SE.seek :beamBreak @15:41:01.27 SE.configure :default @15:41:01.41 Elbow.seek 4 @15:41:01.58 Forearm.seek 4 + 300 counts @15:41:01.70 Forearm.reconfigure :loose @15:41:01.74 Hand.close @15:41:02.31 SE.seek :down @15:41:02.50
Forearm.seek :retract @15:41:03.47 Elbow.seek CC @15:41:03.71 SE.stop @15:41:05.10
Elbow.reconfigure :loose @15:41:05.19 Forearm.reconfigure :tight @15:41:05.24 Forearm.seek CC @15:41:06.42 CC.seek :closed @15:41:10.94 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.11 @15:41:10.95 Hand.open @15:41:11.60 Forearm.reconfigure :loose @15:41:11.64 Forearm.seek :retract @15:41:12.61 CTV.dial CSV,avoiding: :intake @15:41:13.95 CSV.dial CTV @15:41:14.27 PS.seek 2.5ml @15:41:40.64 CS.seek 4.5ml @15:41:55.61 delay 8 seconds @15:42:03.63 PSV.dial :air @15:42:04.08 delay 2 seconds @15:42:06.10 PSV.dial :waste2 @15:42:06.84 PS.seek 0ml @15:42:33.14 CBV.dial between PRV and waste1 @15:42:33.47 CSV.dial :air @15:42:33.93 CS.seek 10ml @15:42:48.89 CSV.dial CTV @15:42:49.33 CS.seek 0ml @15:43:16.10 CBV.dial :waste1 @15:43:16.40 delay 5 seconds @15:43:21.43 CTV.dial :air,avoiding: :intake @15:43:22.82 Storing lyfil puck in Collection Clamp to tube 3 @15:43:22.97 SC.reconfigure :tight @15:43:23.02 SC.seek 3 @15:43:26.62 SC.configure :limp @15:43:26.83 Elbow.reconfigure :tight @15:43:26.87 Elbow.seek 3 @15:43:28.65 Forearm.reconfigure :tight @15:43:28.70 Forearm.seek 3 + 450 counts @15:43:29.76 SE.reconfigure :pick @15:43:29.81 SE.seek :beamBreak @15:43:32.33 SE.configure :default @15:43:32.46 Elbow.reconfigure :limp @15:43:32.51 Forearm.reconfigure :loose @15:43:32.55 Forearm.seek 3 @15:43:32.78 Elbow.hold @15:43:32.84 Elbow.reconfigure :tight @15:43:32.93 SE.seek 10.13 Pucks below Top Plate @15:43:33.36 Forearm.force -20 @15:43:33.87 Forearm.seek 3 + 320 counts @15:43:34.14 Forearm.reconfigure :tight @15:43:34.19 Forearm.seek 3 + 450 counts @15:43:34.38 SE.seek 11.13 Pucks below Top Plate @15:43:34.90 Forearm.reconfigure :loose @15:43:34.94 Forearm.seek :retract @15:43:35.88 Elbow.seek CC @15:43:37.54 Elbow.reconfigure :loose @15:43:37.62 Forearm.seek CC @15:43:38.59 Hand.close @15:43:39.17 CC.seek :open @15:43:43.88 Forearm.seek :retract @15:43:44.84 Elbow.reconfigure :tight @15:43:44.89 Elbow.seek 3 @15:43:46.62 Elbow.reconfigure :limp @15:43:46.67 Forearm.seek 3 @15:43:47.68 Elbow.hold @15:43:47.74 Elbow.reconfigure :tight @15:43:47.79 Hand.open @15:43:48.53 Forearm.force -20 @15:43:48.56 Forearm.seek 3 + 315 counts @15:43:48.87 Forearm.reconfigure :tight @15:43:48.91 Forearm.seek 3 + 450 counts @15:43:49.17 SE.seek :down @15:43:50.30
Forearm.reconfigure :loose @15:43:50.34 Forearm.seek :retract @15:43:51.07 SE.stop @15:43:51.27
PSV.dial :air @15:43:52.03 PS.seek 2.5ml @15:44:18.33 delay 1 second @15:44:19.34 PSV.dial :mixloop @15:44:19.78 delay 1 second @15:44:20.80 PSV.dial :air @15:44:21.25 delay 1 second @15:44:22.27 PSV.dial :mixloop @15:44:22.74 delay 1 second @15:44:23.75 PSV.dial :air @15:44:24.20 delay 1 second @15:44:25.22 PS.seek 0ml @15:44:51.58 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @15:44:51.75 Elbow.seek FlushPuck::Garage @15:44:53.65 Elbow.reconfigure :loose @15:44:53.73 Forearm.seek :garage @15:44:54.80 Hand.close @15:44:55.53 Forearm.seek :retract @15:44:56.50 Elbow.reconfigure :tight @15:44:56.54 Elbow.seek PC @15:44:57.77 Elbow.reconfigure :loose @15:44:57.86 Forearm.reconfigure :tight @15:44:57.90 Forearm.seek PC @15:44:59.08 PC.seek :closed @15:45:03.70 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.56 @15:45:03.77 Hand.open @15:45:04.48 Forearm.reconfigure :loose @15:45:04.52 Forearm.seek :retract @15:45:05.48 PRV.dial :air @15:45:05.92 PSV.dial :mixloop @15:45:06.43 PS.seek 0.3ml @15:45:10.33 PSV.dial PSR @15:45:11.41 Pulling 0.4ml of PSR.air @15:45:11.42 PSR.select :air @15:45:11.44 PS.seek 0.7ml @15:45:16.78 delay 3 seconds @15:45:19.90 PSR.select :air @15:45:19.92 PS.seek 2.5ml @15:45:39.11 delay 3 seconds @15:45:42.12 PSV.dial :mixloop @15:45:43.17 PTV.dial :mixing @15:45:43.92 PBV.dial :waste2 @15:45:44.86 PS.seek 0ml @15:46:11.23 Garaging FlushPuck in Processing Clamp @15:46:11.30 Elbow.reconfigure :tight @15:46:11.35 Elbow.seek PC @15:46:11.46 Elbow.reconfigure :loose @15:46:11.56 Forearm.seek PC @15:46:12.51 Hand.close @15:46:13.17 PC.seek :open @15:46:17.87 Forearm.seek :retract @15:46:18.89 Elbow.reconfigure :tight @15:46:18.93 Elbow.seek :garage @15:46:20.27 Elbow.reconfigure :loose @15:46:20.36 Forearm.reconfigure :tight @15:46:20.41 Forearm.seek :garage @15:46:21.40 Hand.open @15:46:22.10 Forearm.reconfigure :loose @15:46:22.14 Forearm.seek :retract @15:46:23.10 Loading da puck from tube 4 into Processing Clamp @15:46:23.31 SC.reconfigure :tight @15:46:23.36 SC.seek 4 @15:46:26.89 SC.configure :limp @15:46:27.08 Elbow.reconfigure :tight @15:46:27.13 Elbow.seek 4 @15:46:28.98 Forearm.reconfigure :tight @15:46:29.02 Forearm.seek 4 + 450 counts @15:46:30.12 SE.reconfigure :pick @15:46:30.17 SE.seek :beamBreak @15:46:31.86 SE.configure :default @15:46:32.01 Elbow.seek 4 @15:46:32.17 Forearm.seek 4 + 300 counts @15:46:32.37 Forearm.reconfigure :loose @15:46:32.41 Hand.close @15:46:33.06 SE.seek :down @15:46:33.24
Forearm.seek :retract @15:46:34.20 Elbow.seek PC @15:46:34.49 SE.stop @15:46:36.74
Elbow.reconfigure :loose @15:46:36.82 Forearm.reconfigure :tight @15:46:36.86 Forearm.seek PC @15:46:38.06 PC.seek :closed @15:46:42.67 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.60 @15:46:42.68 Hand.open @15:46:43.35 Forearm.reconfigure :loose @15:46:43.39 Forearm.seek :retract @15:46:44.36 Heating Puck.from 4, :da in Processing Clamp to 28C for 2:00:00 @15:46:44.37 PH.seek 28C,5:00 @15:47:14.79 PH.hold 2:00:00,28C @15:47:14.83 delay 1:30 @15:48:44.87 PSR.select :air @15:48:44.89 PSV.dial PSR @15:48:45.99 Pulling 0.5ml of PSR.wash @15:48:46.00 PSR.select :wash @15:48:46.11 PS.seek 0.5ml @15:48:52.17 delay 5 seconds @15:48:57.19 PSR.select :air @15:48:57.30 PS.seek 2ml @15:49:13.92 PTV.dial :puck @15:49:14.65 PSV.dial PTV @15:49:15.53 PBV.dial :waste1 @15:49:16.92 PS.seek 1.5ml @15:49:22.82 delay 3 seconds @15:49:25.84 PBV.dial between PSV and waste1 @15:49:26.21 PS.seek 0ml @15:49:42.35 delay 5:00 @15:54:42.38 recoverPSRreagent08 PTV.dial :puck @15:54:42.39 PBV.dial :waste1 @15:54:42.76 delay 30 seconds @15:55:12.78 PBV.dial between PSV and waste1 @15:55:13.13 PSR.select :air @15:55:13.15 PSV.dial PSR @15:55:13.90 PS.reconfigure :fast @15:55:13.94 PS.seek 2.5ml @15:55:37.69 PSV.dial PTV @15:55:38.46 delay 5 seconds @15:55:43.47 PSV.dial PSR @15:55:44.24 delay 5 seconds @15:55:49.26 PSV.dial PTV @15:55:50.02 delay 5 seconds @15:55:55.04 PSV.dial :air @15:55:55.79 PS.seek 0ml @15:56:18.69 PTV.dial :PRVmixing,via: PRV @15:56:20.00 PSV.dial :mixloop @15:56:20.45 PRV.dial :ab1 @15:56:21.19 PS.reconfigure :slow @15:56:21.24 PS.seek 0.15ml @15:56:24.88 delay 2 seconds @15:56:26.89 CBV.dial PRV @15:56:26.92
PRV.dial CBV @15:56:27.47 PS.seek 0.35ml @15:56:32.09 delay 2 seconds @15:56:34.11 CBV.dial between PRV and PSV @15:56:34.42 PRV.dial :air @15:56:34.87 PS.seek 0.9ml @15:56:46.59 PRV.dial :waste2 @15:56:47.34 PS.seek 0.5ml @15:56:55.97 addPMLReagent08 @15:56:55.98 PSV.dial :air @15:56:56.44 PS.reconfigure :default @15:56:56.49 PS.seek 2ml @15:57:12.56 PSV.dial :PML @15:57:13.02 PTV.dial :mixing @15:57:13.77 PBV.dial :waste1 @15:57:14.18 PS.seek 1.85ml @15:57:16.53 PBV.dial between PSV and waste1 @15:57:16.90 PS.seek 0ml @15:57:36.62 delay 2 seconds @15:57:38.64 Beginning samplePassC @15:57:38.66 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @15:57:38.82 Elbow.reconfigure :tight @15:57:38.86 Elbow.seek FlushPuck::Garage @15:57:40.23 Elbow.reconfigure :loose @15:57:40.31 Forearm.seek :garage @15:57:41.27 Hand.close @15:57:42.02 Forearm.seek :retract @15:57:42.97 Elbow.reconfigure :tight @15:57:43.01 Elbow.seek CC @15:57:45.94 Elbow.reconfigure :loose @15:57:46.04 Forearm.reconfigure :tight @15:57:46.08 Forearm.seek CC @15:57:47.28 CC.seek :closed @15:57:51.81 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.36 @15:57:51.88 Hand.open @15:57:52.59 Forearm.reconfigure :loose @15:57:52.63 Forearm.seek :retract @15:57:53.58 PSV.dial PSR @15:57:54.69 Pulling 1.5ml of PSR.flush @15:57:54.70 PSR.select :flush @15:57:54.82 PS.seek 1.5ml @15:58:10.91 delay 5 seconds @15:58:15.93 PSV.dial :waste1 @15:58:16.84 PS.seek 0ml @15:58:33.00 PSV.dial PSR @15:58:33.45 PSR.select :air @15:58:33.56 PS.seek 2.5ml @15:58:59.88 PRV.dial CBV,avoiding: :ab2 @15:58:59.90
CBV.dial PRV @15:59:01.51 PSV.dial :mixloop @15:59:02.55 CTV.dial :air,avoiding: :intake @15:59:02.56 PTV.dial :PRVmixing @15:59:03.32 PS.seek 0ml @15:59:29.84 PSV.dial :air @15:59:30.44 PS.seek 2.5ml @15:59:56.75 PSV.dial :mixloop @15:59:57.19 PS.seek 0ml @16:00:23.59 PSV.dial :air @16:00:24.05 PS.seek 2.5ml @16:00:50.62 PSV.dial :mixloop @16:00:51.08 PS.seek 0ml @16:01:17.50 Garaging FlushPuck in Collection Clamp @16:01:17.57 Elbow.reconfigure :tight @16:01:17.61 Elbow.seek CC @16:01:17.72 Elbow.reconfigure :loose @16:01:17.81 Forearm.seek CC @16:01:18.78 Hand.close @16:01:19.41 CC.seek :open @16:01:24.04 Forearm.seek :retract @16:01:25.02 Elbow.reconfigure :tight @16:01:25.06 Elbow.seek :garage @16:01:28.02 Elbow.reconfigure :loose @16:01:28.11 Forearm.reconfigure :tight @16:01:28.15 Forearm.seek :garage @16:01:29.08 Hand.open @16:01:29.71 Forearm.reconfigure :loose @16:01:29.76 Forearm.seek :retract @16:01:30.71 delayUntil "16:05:38.63" @16:01:30.73 Whole Cell Archive sampling 1000ml @16:01:30.75 Loading wcr puck from tube 4 into Collection Clamp @16:01:31.19 Elbow.reconfigure :tight @16:01:31.24 Elbow.seek 4 @16:01:33.16 Forearm.reconfigure :tight @16:01:33.21 Forearm.seek 4 + 450 counts @16:01:34.22 SE.reconfigure :pick @16:01:34.28 SE.seek :beamBreak @16:01:36.17 SE.configure :default @16:01:36.31 Elbow.seek 4 @16:01:36.48 Forearm.seek 4 + 300 counts @16:01:36.68 Forearm.reconfigure :loose @16:01:36.73 Hand.close @16:01:37.29 SE.seek :down @16:01:37.48 Forearm.seek :retract @16:01:38.44 Elbow.seek CC @16:01:38.80 SE.stop @16:01:40.13 Elbow.reconfigure :loose @16:01:40.23 Forearm.reconfigure :tight @16:01:40.27 Forearm.seek CC @16:01:41.53 CC.seek :closed @16:01:46.04 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.21 @16:01:46.06 Hand.open @16:01:47.18 Forearm.reconfigure :loose @16:01:47.23 Forearm.seek :retract @16:01:48.20 collectSample 1000ml w/bubble point of 35psi @16:01:48.22 Priming 7ml @16:01:48.23 Intake.select :close @16:01:48.24 Exhaust.select :close @16:01:48.25 SS.hold @16:01:48.44 Calibrating pressure sensors @16:01:48.45 CTV.dial :air @16:01:48.47 SSV.dial :spit,avoiding: :exhaust @16:01:48.48 SS.reconfigure :fast @16:01:48.53 SS.seek 5ml @16:01:51.23 SSV.dial CBV @16:01:51.25 CBV.dial SSV @16:01:52.58 delay 3.8 seconds @16:01:56.52 SSV.dial :spit @16:01:57.83 SS.seek 0ml @16:02:00.69 SSV.dial CBV @16:02:02.01 CTV.dial :intake @16:02:02.78 SSV.dial CBV @16:02:02.80 CBV.dial SSV @16:02:02.82 Allowing 40s for 7.0ml of remaining 7.0ml @16:02:02.83 Intake.select :open @16:02:04.98 Intake @15.2psia, vacuum from 9.7 to 12.7psi @16:02:05.00 SS.reconfigure :inhaling 2.20ml/s @16:02:05.04 SS.seek 7ml @16:02:11.86 Stroke completed -- waiting for pressure to equalize ... @16:02:11.87 SS.reconfigure :exhale @16:02:11.92 SS.hold @16:02:20.47 Intake.select :close @16:02:20.61 SSV.dial :exhaust @16:02:21.38 Exhaust.select :open @16:02:21.49 SS.seek 0ml @16:02:25.80 Exhaust.select :close @16:02:25.91 Sampling 1000.0ml @16:02:25.92 Intake.select :close @16:02:25.93 Exhaust.select :close @16:02:25.94 SS.reconfigure :default @16:02:25.98 SS.hold @16:02:26.17 CTV.dial :intake @16:02:26.19 SSV.dial CBV @16:02:26.21 CBV.dial SSV @16:02:26.97 Allowing 130s for 25.0ml of remaining 1000.0ml @16:02:26.99 Intake.select :open @16:02:29.14 Intake @15.6psia, vacuum from 10.0 to 13.0psi @16:02:29.16 SS.reconfigure :inhaling 2.20ml/s @16:02:29.20 SS.seek 25ml @16:02:36.57 Vacuum too high -- waiting up to 40 seconds @16:02:36.59 SS.reconfigure :exhale @16:02:36.63 SS.hold @16:02:39.08 Intake @13.7psia, vacuum from 8.2 to 11.2psi @16:02:39.10 SS.reconfigure :inhaling 1.98ml/s @16:02:39.14 SS.seek 25ml @16:02:47.77 Stroke completed -- waiting for pressure to equalize ... @16:02:47.78 SS.reconfigure :exhale @16:02:47.82 SS.hold @16:02:56.37 Intake.select :close @16:02:56.51 SSV.dial :exhaust @16:02:57.25 Exhaust.select :open @16:02:57.36 SS.seek 0ml @16:03:06.20 Exhaust.select :close @16:03:06.31 SSV.dial CBV @16:03:06.33 CBV.dial SSV @16:03:07.08 Allowing 130s for 25.0ml of remaining 975.0ml @16:03:07.57 Intake.select :open @16:03:09.72 Intake @15.9psia, vacuum from 10.0 to 13.0psi @16:03:09.74 SS.reconfigure :inhaling 1.98ml/s @16:03:09.78 SS.seek 25ml @16:03:19.26 Vacuum too high -- waiting up to 40 seconds @16:03:19.27 SS.reconfigure :exhale @16:03:19.32 SS.hold @16:03:19.94 Intake @13.1psia, vacuum from 7.6 to 10.6psi @16:03:19.96 SS.reconfigure :inhaling 1.79ml/s @16:03:20.00 SS.seek 25ml @16:03:28.09 Stroke completed -- waiting for pressure to equalize ... @16:03:28.11 SS.reconfigure :exhale @16:03:28.15 SS.hold @16:03:36.61 Intake.select :close @16:03:36.75 SSV.dial :exhaust @16:03:37.50 Exhaust.select :open @16:03:37.61 SS.seek 0ml @16:03:46.45 Exhaust.select :close @16:03:46.56 SSV.dial CBV @16:03:46.58 CBV.dial SSV @16:03:47.33 Allowing 130s for 25.0ml of remaining 950.0ml @16:03:47.34 Intake.select :open @16:03:49.50 Intake @16.0psia, vacuum from 10.0 to 13.0psi @16:03:49.52 SS.reconfigure :inhaling 1.79ml/s @16:03:49.56 SS.seek 25ml @16:03:59.95 Vacuum too high -- waiting up to 40 seconds @16:03:59.96 SS.reconfigure :exhale @16:04:00.00 SS.hold @16:04:00.86 Intake @13.3psia, vacuum from 7.8 to 10.8psi @16:04:00.88 SS.reconfigure :inhaling 1.61ml/s @16:04:00.92 SS.seek 25ml @16:04:09.41 Stroke completed -- waiting for pressure to equalize ... @16:04:09.42 SS.reconfigure :exhale @16:04:09.46 SS.hold @16:04:15.86 Intake.select :close @16:04:16.00 SSV.dial :exhaust @16:04:16.74 Exhaust.select :open @16:04:16.85 SS.seek 0ml @16:04:25.67 Exhaust.select :close @16:04:25.78 SSV.dial CBV @16:04:25.80 CBV.dial SSV @16:04:26.57 Allowing 130s for 25.0ml of remaining 925.0ml @16:04:26.58 Intake.select :open @16:04:28.73 Intake @15.9psia, vacuum from 10.0 to 13.0psi @16:04:28.75 SS.reconfigure :inhaling 1.61ml/s @16:04:28.79 SS.seek 25ml @16:04:41.32 Vacuum too high -- waiting up to 40 seconds @16:04:41.33 SS.reconfigure :exhale @16:04:41.38 SS.hold @16:04:42.47 Intake @13.3psia, vacuum from 7.8 to 10.8psi @16:04:42.49 SS.reconfigure :inhaling 1.45ml/s @16:04:42.53 SS.seek 25ml @16:04:50.01 Stroke completed -- waiting for pressure to equalize ... @16:04:50.03 SS.reconfigure :exhale @16:04:50.07 SS.hold @16:04:56.46 Intake.select :close @16:04:56.60 SSV.dial :exhaust @16:04:57.83 Exhaust.select :open @16:04:57.94 SS.seek 0ml @16:05:07.00 Exhaust.select :close @16:05:07.11 SSV.dial CBV @16:05:07.13 CBV.dial SSV @16:05:07.89 Allowing 130s for 25.0ml of remaining 900.0ml @16:05:07.91 Intake.select :open @16:05:10.06 Intake @15.9psia, vacuum from 10.0 to 13.0psi @16:05:10.08 SS.reconfigure :inhaling 1.45ml/s @16:05:10.13 SS.seek 25ml @16:05:24.66 Vacuum too high -- waiting up to 40 seconds @16:05:24.68 SS.reconfigure :exhale @16:05:24.72 SS.hold @16:05:25.48 Intake @13.3psia, vacuum from 7.8 to 10.8psi @16:05:25.50 SS.reconfigure :inhaling 1.30ml/s @16:05:25.54 SS.seek 25ml @16:05:32.08 Stroke completed -- waiting for pressure to equalize ... @16:05:32.09 SS.reconfigure :exhale @16:05:32.14 SS.hold @16:05:37.67 Intake.select :close @16:05:37.82 SSV.dial :exhaust @16:05:38.56 Exhaust.select :open @16:05:38.64 recoverPRVreagent08 @16:05:38.65 PTV.dial :mixing @16:05:38.69 SS.seek 0ml @16:05:39.41 PBV.dial :waste1 @16:05:39.79 delay 30 seconds @16:05:47.50 Exhaust.select :close @16:05:47.61 SSV.dial CBV @16:05:47.64 CBV.dial SSV @16:05:48.38 Allowing 130s for 25.0ml of remaining 875.0ml @16:05:48.39 Intake.select :open @16:05:50.54 Intake @16.0psia, vacuum from 10.0 to 13.0psi @16:05:50.56 SS.reconfigure :inhaling 1.30ml/s @16:05:50.60 SS.seek 25ml @16:06:09.81 PBV.dial between PSV and waste1 @16:06:10.18 PSV.dial :air @16:06:10.65 PS.reconfigure :fast @16:06:10.69 PS.seek 2.5ml @16:06:11.19 Vacuum too high -- waiting up to 40 seconds @16:06:11.21 SS.reconfigure :exhale @16:06:11.25 SS.hold @16:06:11.69 Intake @13.3psia, vacuum from 7.8 to 10.8psi @16:06:11.70 SS.reconfigure :inhaling 1.17ml/s @16:06:11.75 SS.seek 25ml @16:06:14.16 Stroke completed -- waiting for pressure to equalize ... @16:06:14.17 SS.reconfigure :exhale @16:06:14.21 SS.hold @16:06:19.78 Intake.select :close @16:06:19.93 SSV.dial :exhaust @16:06:20.67 Exhaust.select :open @16:06:20.78 SS.seek 0ml @16:06:29.59 Exhaust.select :close @16:06:29.70 SSV.dial CBV @16:06:29.72 CBV.dial SSV @16:06:30.48 Allowing 130s for 25.0ml of remaining 850.0ml @16:06:30.50 Intake.select :open @16:06:32.65 Intake @15.9psia, vacuum from 10.0 to 13.0psi @16:06:32.67 SS.reconfigure :inhaling 1.17ml/s @16:06:32.71 SS.seek 25ml @16:06:34.39 PSV.dial :mixloop @16:06:34.87 delay 2 seconds @16:06:36.89 PSV.dial :air @16:06:37.35 delay 2 seconds @16:06:39.37 PSV.dial :mixloop @16:06:39.83 delay 2 seconds @16:06:41.85 PSV.dial :air @16:06:42.32 PS.seek 0ml @16:06:57.00 Stroke completed -- waiting for pressure to equalize ... @16:06:57.01 SS.reconfigure :exhale @16:06:57.05 SS.hold @16:07:02.78 Intake.select :close @16:07:02.93 SSV.dial :exhaust @16:07:03.67 Exhaust.select :open @16:07:03.78 SS.seek 0ml @16:07:05.49 washCycleDA #1 of 2 @16:07:05.50 PSR.select :air @16:07:05.52 PSV.dial PSR @16:07:06.88 Pulling 0.5ml of PSR.wash @16:07:06.89 PSR.select :wash @16:07:07.01 PS.reconfigure :default @16:07:07.05 PS.seek 0.5ml @16:07:12.62 Exhaust.select :close @16:07:12.73 SSV.dial CBV @16:07:12.75 CBV.dial SSV @16:07:13.05 delay 5 seconds @16:07:13.52 Allowing 130s for 25.0ml of remaining 825.0ml @16:07:13.53 Intake.select :open @16:07:15.68 Intake @15.9psia, vacuum from 10.0 to 13.0psi @16:07:15.70 SS.reconfigure :inhaling 1.17ml/s @16:07:15.74 SS.seek 25ml @16:07:18.07 PSR.select :air @16:07:18.19 PS.seek 2ml @16:07:34.33 PTV.dial :puck @16:07:35.07 PSV.dial PTV @16:07:35.81 PBV.dial :waste1 @16:07:36.24 PS.seek 1.5ml @16:07:39.96 Stroke completed -- waiting for pressure to equalize ... @16:07:39.98 SS.reconfigure :exhale @16:07:40.02 SS.hold @16:07:42.21 delay 3 seconds @16:07:45.22 PBV.dial between PSV and waste1 @16:07:45.59 PS.seek 0ml @16:07:46.41 Intake.select :close @16:07:46.56 SSV.dial :exhaust @16:07:47.29 Exhaust.select :open @16:07:47.40 SS.seek 0ml @16:07:56.20 Exhaust.select :close @16:07:56.31 SSV.dial CBV @16:07:56.33 CBV.dial SSV @16:07:57.09 Allowing 130s for 25.0ml of remaining 800.0ml @16:07:57.11 Intake.select :open @16:07:59.26 Intake @16.0psia, vacuum from 10.0 to 13.0psi @16:07:59.28 SS.reconfigure :inhaling 1.17ml/s @16:07:59.32 SS.seek 25ml @16:08:01.74 delay 2:00 @16:08:23.10 Vacuum too high -- waiting up to 40 seconds @16:08:23.11 SS.reconfigure :exhale @16:08:23.15 SS.hold @16:08:24.83 Intake @13.8psia, vacuum from 8.3 to 11.3psi @16:08:24.85 SS.reconfigure :inhaling 1.06ml/s @16:08:24.89 SS.seek 25ml @16:08:25.87 Stroke completed -- waiting for pressure to equalize ... @16:08:25.88 SS.reconfigure :exhale @16:08:25.92 SS.hold @16:08:30.69 Intake.select :close @16:08:30.83 SSV.dial :exhaust @16:08:31.57 Exhaust.select :open @16:08:31.68 SS.seek 0ml @16:08:40.53 Exhaust.select :close @16:08:40.64 SSV.dial CBV @16:08:40.65 CBV.dial SSV @16:08:41.40 Allowing 130s for 25.0ml of remaining 775.0ml @16:08:41.42 Intake.select :open @16:08:43.57 Intake @16.0psia, vacuum from 10.0 to 13.0psi @16:08:43.59 SS.reconfigure :inhaling 1.06ml/s @16:08:43.63 SS.seek 25ml @16:09:10.24 Stroke completed -- waiting for pressure to equalize ... @16:09:10.73 SS.reconfigure :exhale @16:09:10.77 SS.hold @16:09:17.91 Intake.select :close @16:09:18.06 SSV.dial :exhaust @16:09:18.81 Exhaust.select :open @16:09:18.92 SS.seek 0ml @16:09:27.74 Exhaust.select :close @16:09:27.85 SSV.dial CBV @16:09:27.87 CBV.dial SSV @16:09:28.64 Allowing 130s for 25.0ml of remaining 750.0ml @16:09:28.65 Intake.select :open @16:09:30.80 Intake @16.0psia, vacuum from 10.0 to 13.0psi @16:09:30.82 SS.reconfigure :inhaling 1.06ml/s @16:09:30.86 SS.seek 25ml @16:09:57.43 Stroke completed -- waiting for pressure to equalize ... @16:09:57.44 SS.reconfigure :exhale @16:09:57.49 SS.hold @16:10:01.75 recoverPSRreagent08 @16:10:01.77 PTV.dial :puck @16:10:01.78 PBV.dial :waste1 @16:10:02.13 delay 30 seconds @16:10:04.92 Intake.select :close @16:10:05.06 SSV.dial :exhaust @16:10:05.81 Exhaust.select :open @16:10:05.92 SS.seek 0ml @16:10:14.70 Exhaust.select :close @16:10:14.80 SSV.dial CBV @16:10:14.82 CBV.dial SSV @16:10:15.59 Allowing 130s for 25.0ml of remaining 725.0ml @16:10:15.60 Intake.select :open @16:10:17.76 Intake @16.0psia, vacuum from 10.0 to 13.0psi @16:10:17.78 SS.reconfigure :inhaling 1.06ml/s @16:10:17.82 SS.seek 25ml @16:10:32.14 PBV.dial between PSV and waste1 @16:10:32.50 PSR.select :air @16:10:32.52 PSV.dial PSR @16:10:33.41 PS.reconfigure :fast @16:10:33.46 PS.seek 2.5ml @16:10:44.41 Stroke completed -- waiting for pressure to equalize ... @16:10:44.43 SS.reconfigure :exhale @16:10:44.47 SS.hold @16:10:52.93 Intake.select :close @16:10:53.07 SSV.dial :exhaust @16:10:53.82 Exhaust.select :open @16:10:53.93 SS.seek 0ml @16:10:57.33 PSV.dial PTV @16:10:58.19 delay 5 seconds @16:11:02.79 Exhaust.select :close @16:11:02.90 SSV.dial CBV @16:11:02.94 CBV.dial SSV @16:11:03.20 PSV.dial PSR @16:11:03.67 Allowing 130s for 25.0ml of remaining 700.0ml @16:11:03.68 Intake.select :open @16:11:03.95 delay 5 seconds @16:11:05.83 Intake @16.0psia, vacuum from 10.0 to 13.0psi @16:11:05.85 SS.reconfigure :inhaling 1.06ml/s @16:11:05.89 SS.seek 25ml @16:11:08.97 PSV.dial PTV @16:11:09.73 delay 5 seconds @16:11:14.75 PSV.dial :air @16:11:15.63 PS.seek 0ml @16:11:30.92 Vacuum too high -- waiting up to 40 seconds @16:11:30.93 SS.reconfigure :exhale @16:11:30.97 SS.hold @16:11:33.40 Intake @14.2psia, vacuum from 8.7 to 11.7psi @16:11:33.42 SS.reconfigure :inhaling 0.95ml/s @16:11:33.46 SS.seek 25ml @16:11:36.03 Stroke completed -- waiting for pressure to equalize ... @16:11:36.04 SS.reconfigure :exhale @16:11:36.08 SS.hold @16:11:38.83 washCycleDA #2 of 2 PSR.select :air @16:11:38.85 PSV.dial PSR @16:11:40.24 Pulling 0.5ml of PSR.wash @16:11:40.25 PSR.select :wash @16:11:40.36 PS.reconfigure :default @16:11:40.40 PS.seek 0.5ml @16:11:40.92 Intake.select :close @16:11:41.07 SSV.dial :exhaust @16:11:41.84 Exhaust.select :open @16:11:41.95 SS.seek 0ml @16:11:46.27 delay 5 seconds @16:11:50.71 Exhaust.select :close @16:11:50.82 SSV.dial CBV @16:11:50.84 CBV.dial SSV @16:11:51.29 PSR.select :air @16:11:51.40 PS.seek 2ml @16:11:51.62 Allowing 130s for 25.0ml of remaining 675.0ml @16:11:51.63 Intake.select :open @16:11:53.79 Intake @16.1psia, vacuum from 10.0 to 13.0psi @16:11:53.81 SS.reconfigure :inhaling 0.95ml/s @16:11:53.85 SS.seek 25ml @16:12:07.48 PTV.dial :puck @16:12:07.49 PSV.dial PTV @16:12:08.25 PBV.dial :waste1 @16:12:08.65 PS.seek 1.5ml @16:12:14.58 delay 3 seconds @16:12:17.59 PBV.dial between PSV and waste1 @16:12:17.96 PS.seek 0ml @16:12:22.80 Stroke completed -- waiting for pressure to equalize ... @16:12:22.81 SS.reconfigure :exhale @16:12:22.86 SS.hold @16:12:30.34 Intake.select :close @16:12:30.48 SSV.dial :exhaust @16:12:31.23 Exhaust.select :open @16:12:31.38 SS.seek 0ml @16:12:34.01 delay 2:00 @16:12:40.21 Exhaust.select :close @16:12:40.32 SSV.dial CBV @16:12:40.35 CBV.dial SSV @16:12:41.09 Allowing 130s for 25.0ml of remaining 650.0ml @16:12:41.10 Intake.select :open @16:12:43.25 Intake @16.1psia, vacuum from 10.0 to 13.0psi @16:12:43.27 SS.reconfigure :inhaling 0.95ml/s @16:12:43.31 SS.seek 25ml @16:13:11.19 Vacuum too high -- waiting up to 40 seconds @16:13:11.20 SS.reconfigure :exhale @16:13:11.24 SS.hold @16:13:13.74 Intake @14.1psia, vacuum from 8.6 to 11.6psi @16:13:13.76 SS.reconfigure :inhaling 0.87ml/s @16:13:13.80 SS.seek 25ml @16:13:15.35 Stroke completed -- waiting for pressure to equalize ... @16:13:15.36 SS.reconfigure :exhale @16:13:15.40 SS.hold @16:13:20.65 Intake.select :close @16:13:20.79 SSV.dial :exhaust @16:13:21.54 Exhaust.select :open @16:13:21.65 SS.seek 0ml @16:13:30.74 Exhaust.select :close @16:13:30.85 SSV.dial CBV @16:13:30.88 CBV.dial SSV @16:13:31.62 Allowing 130s for 25.0ml of remaining 625.0ml @16:13:31.63 Intake.select :open @16:13:33.78 Intake @15.9psia, vacuum from 10.0 to 13.0psi @16:13:33.80 SS.reconfigure :inhaling 0.87ml/s @16:13:33.84 SS.seek 25ml @16:14:05.07 Stroke completed -- waiting for pressure to equalize ... @16:14:05.08 SS.reconfigure :exhale @16:14:05.12 SS.hold @16:14:12.49 Intake.select :close @16:14:12.63 SSV.dial :exhaust @16:14:13.40 Exhaust.select :open @16:14:13.51 SS.seek 0ml @16:14:22.38 Exhaust.select :close @16:14:22.49 SSV.dial CBV @16:14:22.52 CBV.dial SSV @16:14:23.27 Allowing 130s for 25.0ml of remaining 600.0ml @16:14:23.28 Intake.select :open @16:14:25.44 Intake @15.9psia, vacuum from 10.0 to 13.0psi @16:14:25.46 SS.reconfigure :inhaling 0.87ml/s @16:14:25.50 SS.seek 25ml @16:14:34.04 recoverPSRreagent08 @16:14:34.05 PTV.dial :puck @16:14:34.06 PBV.dial :waste1 @16:14:34.42 delay 30 seconds @16:14:57.44 Stroke completed -- waiting for pressure to equalize ... @16:14:57.45 SS.reconfigure :exhale @16:14:57.50 SS.hold @16:15:04.43 PBV.dial between PSV and waste1 @16:15:04.83 PSR.select :air @16:15:04.87 PSV.dial PSR @16:15:04.95 Intake.select :close @16:15:05.10 SSV.dial :exhaust @16:15:05.66 PS.reconfigure :fast @16:15:05.70 PS.seek 2.5ml @16:15:05.86 Exhaust.select :open @16:15:05.97 SS.seek 0ml @16:15:14.99 Exhaust.select :close @16:15:15.10 SSV.dial CBV @16:15:15.11 CBV.dial SSV @16:15:15.88 Allowing 130s for 25.0ml of remaining 575.0ml @16:15:15.89 Intake.select :open @16:15:18.05 Intake @15.9psia, vacuum from 10.0 to 13.0psi @16:15:18.07 SS.reconfigure :inhaling 0.87ml/s @16:15:18.11 SS.seek 25ml @16:15:29.32 PSV.dial PTV @16:15:30.09 delay 5 seconds @16:15:35.11 PSV.dial PSR @16:15:35.85 delay 5 seconds @16:15:40.87 PSV.dial PTV @16:15:41.62 delay 5 seconds @16:15:46.64 PSV.dial :air @16:15:47.51 PS.seek 0ml @16:15:48.98 Stroke completed -- waiting for pressure to equalize ... @16:15:49.00 SS.reconfigure :exhale @16:15:49.04 SS.hold @16:15:57.78 Intake.select :close @16:15:57.92 SSV.dial :exhaust @16:15:58.69 Exhaust.select :open @16:15:58.80 SS.seek 0ml @16:16:07.66 Exhaust.select :close @16:16:07.76 SSV.dial CBV @16:16:07.81 CBV.dial SSV @16:16:08.54 Allowing 130s for 25.0ml of remaining 550.0ml @16:16:08.55 Intake.select :open @16:16:10.71 Intake @15.9psia, vacuum from 10.0 to 13.0psi @16:16:10.73 SS.reconfigure :inhaling 0.87ml/s @16:16:10.77 SS.seek 25ml @16:16:10.88 getPMLReagent08 @16:16:10.89 PRV.dial :air @16:16:11.34 delay 5 seconds @16:16:16.35 PTV.dial :PRVmixing @16:16:17.68 delay 5 seconds @16:16:22.70 PRV.dial :ab2 @16:16:23.15 PTV.dial :PRVmixing @16:16:23.16 PSV.dial :mixloop @16:16:23.61 PS.reconfigure :default @16:16:23.65 PS.seek 0.3ml @16:16:27.54 delay 2 seconds @16:16:29.56 PRV.dial :air @16:16:30.05 PS.seek 0.55ml @16:16:33.42 addPMLReagent08 @16:16:33.43 PSV.dial :air @16:16:33.89 PS.seek 2ml @16:16:42.00 Vacuum too high -- waiting up to 40 seconds @16:16:42.01 SS.reconfigure :exhale @16:16:42.05 SS.hold @16:16:45.05 Intake @14.3psia, vacuum from 8.8 to 11.8psi @16:16:45.07 SS.reconfigure :inhaling 0.78ml/s @16:16:45.11 SS.seek 25ml @16:16:45.41 Stroke completed -- waiting for pressure to equalize ... @16:16:45.42 SS.reconfigure :exhale @16:16:45.46 SS.hold @16:16:49.54 PSV.dial :PML @16:16:49.99 PTV.dial :mixing @16:16:50.72 PBV.dial :waste1 @16:16:51.14 PS.seek 1.85ml @16:16:51.87 Intake.select :close @16:16:52.01 SSV.dial :exhaust @16:16:52.75 Exhaust.select :open @16:16:52.86 SS.seek 0ml @16:16:53.41 PBV.dial between PSV and waste1 @16:16:53.80 PS.seek 0ml @16:17:01.61 Exhaust.select :close @16:17:01.72 SSV.dial CBV @16:17:01.74 CBV.dial SSV @16:17:02.50 Allowing 130s for 25.0ml of remaining 525.0ml @16:17:02.51 Intake.select :open @16:17:04.67 Intake @16.0psia, vacuum from 10.0 to 13.0psi @16:17:04.69 SS.reconfigure :inhaling 0.78ml/s @16:17:04.73 SS.seek 25ml @16:17:13.54 delay 2 seconds @16:17:15.56 delay 8:00 @16:17:39.86 Stroke completed -- waiting for pressure to equalize ... @16:17:39.87 SS.reconfigure :exhale @16:17:39.91 SS.hold @16:17:48.90 Intake.select :close @16:17:49.04 SSV.dial :exhaust @16:17:49.78 Exhaust.select :open @16:17:49.89 SS.seek 0ml @16:17:58.80 Exhaust.select :close @16:17:58.90 SSV.dial CBV @16:17:58.93 CBV.dial SSV @16:17:59.67 Allowing 130s for 25.0ml of remaining 500.0ml @16:17:59.69 Intake.select :open @16:18:01.84 Intake @16.0psia, vacuum from 10.0 to 13.0psi @16:18:01.86 SS.reconfigure :inhaling 0.78ml/s @16:18:01.90 SS.seek 25ml @16:18:36.68 Stroke completed -- waiting for pressure to equalize ... @16:18:36.70 SS.reconfigure :exhale @16:18:36.74 SS.hold @16:18:46.58 Intake.select :close @16:18:46.72 SSV.dial :exhaust @16:18:47.47 Exhaust.select :open @16:18:47.58 SS.seek 0ml @16:18:56.67 Exhaust.select :close @16:18:56.78 SSV.dial CBV @16:18:56.80 CBV.dial SSV @16:18:57.56 Allowing 130s for 25.0ml of remaining 475.0ml @16:18:57.58 Intake.select :open @16:18:59.73 Intake @16.1psia, vacuum from 10.0 to 13.0psi @16:18:59.75 SS.reconfigure :inhaling 0.78ml/s @16:18:59.79 SS.seek 25ml @16:19:30.71 Vacuum too high -- waiting up to 40 seconds @16:19:30.72 SS.reconfigure :exhale @16:19:30.76 SS.hold @16:19:34.31 Intake @14.7psia, vacuum from 9.2 to 12.2psi @16:19:34.33 SS.reconfigure :inhaling 0.70ml/s @16:19:34.37 SS.seek 25ml @16:19:38.82 Stroke completed -- waiting for pressure to equalize ... @16:19:38.84 SS.reconfigure :exhale @16:19:38.88 SS.hold @16:19:45.28 Intake.select :close @16:19:45.42 SSV.dial :exhaust @16:19:46.17 Exhaust.select :open @16:19:46.28 SS.seek 0ml @16:19:55.45 Exhaust.select :close @16:19:55.56 SSV.dial CBV @16:19:55.59 CBV.dial SSV @16:19:56.33 Allowing 130s for 25.0ml of remaining 450.0ml @16:19:56.34 Intake.select :open @16:19:58.51 Intake @16.1psia, vacuum from 10.0 to 13.0psi @16:19:58.53 SS.reconfigure :inhaling 0.70ml/s @16:19:58.57 SS.seek 25ml @16:20:35.79 Stroke completed -- waiting for pressure to equalize ... @16:20:35.80 SS.reconfigure :exhale @16:20:35.85 SS.hold @16:20:45.61 Intake.select :close @16:20:45.75 SSV.dial :exhaust @16:20:46.50 Exhaust.select :open @16:20:46.61 SS.seek 0ml @16:20:55.73 Exhaust.select :close @16:20:55.84 SSV.dial CBV @16:20:55.86 CBV.dial SSV @16:20:56.62 Allowing 130s for 25.0ml of remaining 425.0ml @16:20:56.64 Intake.select :open @16:20:58.79 Intake @16.1psia, vacuum from 10.0 to 13.0psi @16:20:58.81 SS.reconfigure :inhaling 0.70ml/s @16:20:58.85 SS.seek 25ml @16:21:36.15 Stroke completed -- waiting for pressure to equalize ... @16:21:36.16 SS.reconfigure :exhale @16:21:36.20 SS.hold @16:21:47.33 Intake.select :close @16:21:47.47 SSV.dial :exhaust @16:21:48.21 Exhaust.select :open @16:21:48.32 SS.seek 0ml @16:21:57.51 Exhaust.select :close @16:21:57.62 SSV.dial CBV @16:21:57.65 CBV.dial SSV @16:21:58.39 Allowing 130s for 25.0ml of remaining 400.0ml @16:21:58.40 Intake.select :open @16:22:00.55 Intake @16.1psia, vacuum from 10.0 to 13.0psi @16:22:00.57 SS.reconfigure :inhaling 0.70ml/s @16:22:00.61 SS.seek 25ml @16:22:36.02 Vacuum too high -- waiting up to 40 seconds @16:22:36.03 SS.reconfigure :exhale @16:22:36.07 SS.hold @16:22:40.93 Intake @15.0psia, vacuum from 9.5 to 12.5psi @16:22:40.95 SS.reconfigure :inhaling 0.63ml/s @16:22:40.99 SS.seek 25ml @16:22:43.32 Stroke completed -- waiting for pressure to equalize ... @16:22:43.33 SS.reconfigure :exhale @16:22:43.38 SS.hold @16:22:49.78 Intake.select :close @16:22:49.92 SSV.dial :exhaust @16:22:50.67 Exhaust.select :open @16:22:50.78 SS.seek 0ml @16:22:59.62 Exhaust.select :close @16:22:59.72 SSV.dial CBV @16:22:59.75 CBV.dial SSV @16:23:00.49 Allowing 130s for 25.0ml of remaining 375.0ml @16:23:00.51 Intake.select :open @16:23:02.66 Intake @16.0psia, vacuum from 10.0 to 13.0psi @16:23:02.68 SS.reconfigure :inhaling 0.63ml/s @16:23:02.72 SS.seek 25ml @16:23:45.23 Stroke completed -- waiting for pressure to equalize ... @16:23:45.24 SS.reconfigure :exhale @16:23:45.28 SS.hold @16:23:55.07 Intake.select :close @16:23:55.21 SSV.dial :exhaust @16:23:55.98 Exhaust.select :open @16:23:56.09 SS.seek 0ml @16:24:05.21 Exhaust.select :close @16:24:05.32 SSV.dial CBV @16:24:05.34 CBV.dial SSV @16:24:06.10 Allowing 130s for 25.0ml of remaining 350.0ml @16:24:06.12 Intake.select :open @16:24:08.27 Intake @16.1psia, vacuum from 10.0 to 13.0psi @16:24:08.29 SS.reconfigure :inhaling 0.63ml/s @16:24:08.33 SS.seek 25ml @16:24:49.36 Vacuum too high -- waiting up to 40 seconds @16:24:49.37 SS.reconfigure :exhale @16:24:49.41 SS.hold @16:24:53.59 Intake @15.1psia, vacuum from 9.6 to 12.6psi @16:24:53.61 SS.reconfigure :inhaling 0.57ml/s @16:24:53.65 SS.seek 25ml @16:24:54.57 Stroke completed -- waiting for pressure to equalize ... @16:24:54.58 SS.reconfigure :exhale @16:24:54.62 SS.hold @16:25:01.03 Intake.select :close @16:25:01.17 SSV.dial :exhaust @16:25:01.91 Exhaust.select :open @16:25:02.03 SS.seek 0ml @16:25:10.88 Exhaust.select :close @16:25:10.99 SSV.dial CBV @16:25:11.01 CBV.dial SSV @16:25:11.79 Allowing 130s for 25.0ml of remaining 325.0ml @16:25:11.80 Intake.select :open @16:25:13.96 Intake @16.1psia, vacuum from 10.0 to 13.0psi @16:25:13.97 SS.reconfigure :inhaling 0.57ml/s @16:25:14.02 SS.seek 25ml @16:25:15.57 recoverPRVreagent08 @16:25:15.58 PTV.dial :mixing @16:25:15.59 PBV.dial :waste1 @16:25:15.97 delay 30 seconds @16:25:46.00 PBV.dial between PSV and waste1 @16:25:46.36 PSV.dial :air @16:25:46.83 PS.reconfigure :fast @16:25:46.87 PS.seek 2.5ml @16:26:00.38 Stroke completed -- waiting for pressure to equalize ... @16:26:00.39 SS.reconfigure :exhale @16:26:00.43 SS.hold @16:26:10.23 Intake.select :close @16:26:10.37 SSV.dial :exhaust @16:26:10.77 PSV.dial :mixloop @16:26:11.13 Exhaust.select :open @16:26:11.23 delay 2 seconds @16:26:11.26 SS.seek 0ml @16:26:13.25 PSV.dial :air @16:26:13.72 delay 2 seconds @16:26:15.73 PSV.dial :mixloop @16:26:16.22 delay 2 seconds @16:26:18.27 PSV.dial :air @16:26:18.74 PS.seek 0ml @16:26:20.05 Exhaust.select :close @16:26:20.16 SSV.dial CBV @16:26:20.69 CBV.dial SSV @16:26:21.52 Allowing 130s for 25.0ml of remaining 300.0ml @16:26:21.54 Intake.select :open @16:26:23.69 Intake @16.1psia, vacuum from 10.0 to 13.0psi @16:26:23.71 SS.reconfigure :inhaling 0.57ml/s @16:26:23.75 SS.seek 25ml @16:26:42.28 washCycleDA #1 of 3 @16:26:42.29 PSR.select :air @16:26:42.30 PSV.dial PSR @16:26:43.67 Pulling 0.5ml of PSR.wash @16:26:43.68 PSR.select :wash @16:26:43.80 PS.reconfigure :default @16:26:43.84 PS.seek 0.5ml @16:26:49.75 delay 5 seconds @16:26:54.77 PSR.select :air @16:26:54.88 PS.seek 2ml @16:27:09.91 Stroke completed -- waiting for pressure to equalize ... @16:27:09.93 SS.reconfigure :exhale @16:27:09.97 SS.hold @16:27:10.96 PTV.dial :puck @16:27:11.70 PSV.dial PTV @16:27:12.43 PBV.dial :waste1 @16:27:12.92 PS.seek 1.5ml @16:27:18.93 delay 3 seconds @16:27:21.95 PBV.dial between PSV and waste1 @16:27:22.31 PS.seek 0ml @16:27:22.56 Intake.select :close @16:27:22.71 SSV.dial :exhaust @16:27:23.46 Exhaust.select :open @16:27:23.57 SS.seek 0ml @16:27:32.46 Exhaust.select :close @16:27:32.56 SSV.dial CBV @16:27:32.58 CBV.dial SSV @16:27:33.33 Allowing 130s for 25.0ml of remaining 275.0ml @16:27:33.35 Intake.select :open @16:27:35.50 Intake @16.2psia, vacuum from 10.0 to 13.0psi @16:27:35.52 SS.reconfigure :inhaling 0.57ml/s @16:27:35.56 SS.seek 25ml @16:27:38.65 delay 2:00 @16:28:19.20 Vacuum too high -- waiting up to 40 seconds @16:28:19.21 SS.reconfigure :exhale @16:28:19.25 SS.hold @16:28:24.12 Intake @15.3psia, vacuum from 9.8 to 12.8psi @16:28:24.14 SS.reconfigure :inhaling 0.51ml/s @16:28:24.18 SS.seek 25ml @16:28:27.67 Stroke completed -- waiting for pressure to equalize ... @16:28:27.68 SS.reconfigure :exhale @16:28:27.73 SS.hold @16:28:35.09 Intake.select :close @16:28:35.23 SSV.dial :exhaust @16:28:35.99 Exhaust.select :open @16:28:36.10 SS.seek 0ml @16:28:45.16 Exhaust.select :close @16:28:45.26 SSV.dial CBV @16:28:45.29 CBV.dial SSV @16:28:46.03 Allowing 130s for 25.0ml of remaining 250.0ml @16:28:46.05 Intake.select :open @16:28:48.20 Intake @16.1psia, vacuum from 10.0 to 13.0psi @16:28:48.22 SS.reconfigure :inhaling 0.51ml/s @16:28:48.26 SS.seek 25ml @16:29:38.68 recoverPSRreagent08 @16:29:38.69 PTV.dial :puck @16:29:39.23 Stroke completed -- waiting for pressure to equalize ... @16:29:39.24 PBV.dial :waste1 @16:29:39.26 SS.reconfigure :exhale @16:29:39.35 SS.hold @16:29:39.71 delay 30 seconds @16:29:50.48 Intake.select :close @16:29:50.62 SSV.dial :exhaust @16:29:51.36 Exhaust.select :open @16:29:51.47 SS.seek 0ml @16:30:00.55 Exhaust.select :close @16:30:00.65 SSV.dial CBV @16:30:00.68 CBV.dial SSV @16:30:01.42 Allowing 130s for 25.0ml of remaining 225.0ml @16:30:01.44 Intake.select :open @16:30:03.59 Intake @16.1psia, vacuum from 10.0 to 13.0psi @16:30:03.61 SS.reconfigure :inhaling 0.51ml/s @16:30:03.65 SS.seek 25ml @16:30:09.73 PBV.dial between PSV and waste1 @16:30:10.10 PSR.select :air @16:30:10.12 PSV.dial PSR @16:30:10.87 PS.reconfigure :fast @16:30:10.92 PS.seek 2.5ml @16:30:34.83 PSV.dial PTV @16:30:35.60 delay 5 seconds @16:30:40.62 PSV.dial PSR @16:30:41.38 delay 5 seconds @16:30:46.40 PSV.dial PTV @16:30:47.16 delay 5 seconds @16:30:52.18 PSV.dial :air @16:30:53.06 PS.seek 0ml @16:30:54.36 Stroke completed -- waiting for pressure to equalize ... @16:30:54.37 SS.reconfigure :exhale @16:30:54.41 SS.hold @16:31:06.96 Intake.select :close @16:31:07.10 SSV.dial :exhaust @16:31:07.85 Exhaust.select :open @16:31:07.96 SS.seek 0ml @16:31:16.52 washCycleDA #2 of 3 @16:31:16.53 PSR.select :air @16:31:16.55 PSV.dial PSR @16:31:17.12 Exhaust.select :close @16:31:17.23 SSV.dial CBV @16:31:17.25 CBV.dial SSV @16:31:17.92 Pulling 0.5ml of PSR.wash @16:31:17.93 PSR.select :wash @16:31:18.02 Allowing 130s for 25.0ml of remaining 200.0ml @16:31:18.03 Intake.select :open @16:31:18.09 PS.reconfigure :default @16:31:18.13 PS.seek 0.5ml @16:31:20.23 Intake @16.0psia, vacuum from 10.0 to 13.0psi @16:31:20.25 SS.reconfigure :inhaling 0.51ml/s @16:31:20.29 SS.seek 25ml @16:31:24.05 delay 5 seconds @16:31:29.07 PSR.select :air @16:31:29.19 PS.seek 2ml @16:31:45.36 PTV.dial :puck @16:31:45.38 PSV.dial PTV @16:31:46.13 PBV.dial :waste1 @16:31:46.56 PS.seek 1.5ml @16:31:52.52 delay 3 seconds @16:31:55.54 PBV.dial between PSV and waste1 @16:31:55.92 PS.seek 0ml @16:32:11.18 Stroke completed -- waiting for pressure to equalize ... @16:32:11.20 SS.reconfigure :exhale @16:32:11.24 SS.hold @16:32:12.14 delay 2:00 @16:32:24.01 Intake.select :close @16:32:24.15 SSV.dial :exhaust @16:32:24.90 Exhaust.select :open @16:32:25.01 SS.seek 0ml @16:32:34.12 Exhaust.select :close @16:32:34.23 SSV.dial CBV @16:32:34.26 CBV.dial SSV @16:32:35.01 Allowing 130s for 25.0ml of remaining 175.0ml @16:32:35.03 Intake.select :open @16:32:37.18 Intake @16.2psia, vacuum from 10.0 to 13.0psi @16:32:37.20 SS.reconfigure :inhaling 0.51ml/s @16:32:37.24 SS.seek 25ml @16:33:18.82 Vacuum too high -- waiting up to 40 seconds @16:33:18.83 SS.reconfigure :exhale @16:33:18.87 SS.hold @16:33:24.51 Intake @15.6psia, vacuum from 10.0 to 13.0psi @16:33:24.53 SS.reconfigure :inhaling 0.47ml/s @16:33:24.57 SS.seek 25ml @16:33:34.84 Stroke completed -- waiting for pressure to equalize ... @16:33:34.85 SS.reconfigure :exhale @16:33:34.89 SS.hold @16:33:45.91 Intake.select :close @16:33:46.05 SSV.dial :exhaust @16:33:46.79 Exhaust.select :open @16:33:46.90 SS.seek 0ml @16:33:55.82 Exhaust.select :close @16:33:55.93 SSV.dial CBV @16:33:55.95 CBV.dial SSV @16:33:56.73 Allowing 130s for 25.0ml of remaining 150.0ml @16:33:56.74 Intake.select :open @16:33:58.91 Intake @16.2psia, vacuum from 10.0 to 13.0psi @16:33:58.92 SS.reconfigure :inhaling 0.47ml/s @16:33:58.97 SS.seek 25ml @16:34:12.16 recoverPSRreagent08 @16:34:12.18 PTV.dial :puck @16:34:12.19 PBV.dial :waste1 @16:34:12.55 delay 30 seconds @16:34:42.58 PBV.dial between PSV and waste1 @16:34:42.92 PSR.select :air @16:34:42.94 PSV.dial PSR @16:34:43.82 PS.reconfigure :fast @16:34:43.86 PS.seek 2.5ml @16:34:55.09 Stroke completed -- waiting for pressure to equalize ... @16:34:55.10 SS.reconfigure :exhale @16:34:55.14 SS.hold @16:35:07.58 PSV.dial PTV @16:35:08.30 delay 5 seconds @16:35:10.00 Intake.select :close @16:35:10.14 SSV.dial :exhaust @16:35:10.88 Exhaust.select :open @16:35:11.00 SS.seek 0ml @16:35:13.32 PSV.dial PSR @16:35:14.06 delay 5 seconds @16:35:19.08 PSV.dial PTV @16:35:19.84 delay 5 seconds @16:35:20.12 Exhaust.select :close @16:35:20.23 SSV.dial CBV @16:35:20.26 CBV.dial SSV @16:35:21.00 Allowing 130s for 25.0ml of remaining 125.0ml @16:35:21.01 Intake.select :open @16:35:23.16 Intake @16.2psia, vacuum from 10.0 to 13.0psi @16:35:23.18 SS.reconfigure :inhaling 0.47ml/s @16:35:23.22 SS.seek 25ml @16:35:24.86 PSV.dial :air @16:35:25.62 PS.seek 0ml @16:35:48.95 washCycleDA #3 of 3 @16:35:48.96 PSR.select :air @16:35:48.98 PSV.dial PSR @16:35:50.35 Pulling 0.5ml of PSR.wash @16:35:50.36 PSR.select :wash @16:35:50.48 PS.reconfigure :default @16:35:50.52 PS.seek 0.5ml @16:35:56.49 delay 5 seconds @16:36:01.50 PSR.select :air @16:36:01.62 PS.seek 2ml @16:36:11.60 Vacuum too high -- waiting up to 40 seconds @16:36:11.61 SS.reconfigure :exhale @16:36:11.65 SS.hold @16:36:17.29 Intake @15.7psia, vacuum from 10.0 to 13.0psi @16:36:17.31 SS.reconfigure :inhaling 0.43ml/s @16:36:17.35 SS.seek 25ml @16:36:17.70 PTV.dial :puck @16:36:17.71 PSV.dial PTV @16:36:18.45 PBV.dial :waste1 @16:36:18.87 PS.seek 1.5ml @16:36:24.95 delay 3 seconds @16:36:25.93 Stroke completed -- waiting for pressure to equalize ... @16:36:25.94 SS.reconfigure :exhale @16:36:25.98 SS.hold @16:36:28.02 PBV.dial between PSV and waste1 @16:36:28.38 PS.seek 0ml @16:36:37.21 Intake.select :close @16:36:37.35 SSV.dial :exhaust @16:36:38.10 Exhaust.select :open @16:36:38.21 SS.seek 0ml @16:36:44.55 delay 2:00 @16:36:47.33 Exhaust.select :close @16:36:47.44 SSV.dial CBV @16:36:47.46 CBV.dial SSV @16:36:48.21 Allowing 130s for 25.0ml of remaining 100.0ml @16:36:48.22 Intake.select :open @16:36:50.38 Intake @16.2psia, vacuum from 10.0 to 13.0psi @16:36:50.40 SS.reconfigure :inhaling 0.43ml/s @16:36:50.44 SS.seek 25ml @16:37:52.41 Stroke completed -- waiting for pressure to equalize ... @16:37:52.42 SS.reconfigure :exhale @16:37:52.47 SS.hold @16:38:06.85 Intake.select :close @16:38:06.99 SSV.dial :exhaust @16:38:07.74 Exhaust.select :open @16:38:07.85 SS.seek 0ml @16:38:16.72 Exhaust.select :close @16:38:16.83 SSV.dial CBV @16:38:16.85 CBV.dial SSV @16:38:17.62 Allowing 130s for 25.0ml of remaining 75.0ml @16:38:17.63 Intake.select :open @16:38:19.78 Intake @16.2psia, vacuum from 10.0 to 13.0psi @16:38:19.80 SS.reconfigure :inhaling 0.43ml/s @16:38:19.84 SS.seek 25ml @16:38:44.56 recoverPSRreagent08 @16:38:44.58 PTV.dial :puck @16:38:44.59 PBV.dial :waste1 @16:38:44.93 delay 30 seconds @16:39:14.96 PBV.dial between PSV and waste1 @16:39:15.31 PSR.select :air @16:39:15.33 PSV.dial PSR @16:39:16.09 PS.reconfigure :fast @16:39:16.14 PS.seek 2.5ml @16:39:22.13 Stroke completed -- waiting for pressure to equalize ... @16:39:22.14 SS.reconfigure :exhale @16:39:22.18 SS.hold @16:39:38.36 Intake.select :close @16:39:38.50 SSV.dial :exhaust @16:39:39.25 Exhaust.select :open @16:39:39.36 SS.seek 0ml @16:39:39.79 PSV.dial PTV @16:39:40.56 delay 5 seconds @16:39:45.58 PSV.dial PSR @16:39:46.32 delay 5 seconds @16:39:48.17 Exhaust.select :close @16:39:48.28 SSV.dial CBV @16:39:48.30 CBV.dial SSV @16:39:49.07 Allowing 130s for 25.0ml of remaining 50.0ml @16:39:49.08 Intake.select :open @16:39:51.24 Intake @16.0psia, vacuum from 10.0 to 13.0psi @16:39:51.26 SS.reconfigure :inhaling 0.43ml/s @16:39:51.30 SS.seek 25ml @16:39:51.34 PSV.dial PTV @16:39:52.09 delay 5 seconds @16:39:57.11 PSV.dial :air @16:39:57.98 PS.seek 0ml @16:40:21.26 Gate.power :camera,:ON @16:40:21.27 Mixing DA Substrates @16:40:21.28 PSR.select :air @16:40:21.30 PSV.dial PSR @16:40:22.83 Pulling 0.25ml of PSR.sub1 @16:40:22.86 PSR.select :sub1 @16:40:23.09 PS.reconfigure :default @16:40:23.22 PS.seek 0.25ml @16:40:26.67 delay 5 seconds @16:40:31.74 Pulling 0.25ml of PSR.sub2 @16:40:31.75 PSR.select :sub2 @16:40:31.86 PS.seek 0.5ml @16:40:35.45 delay 5 seconds @16:40:40.47 PSR.select :air @16:40:40.58 PS.seek 2ml @16:40:53.89 Stroke completed -- waiting for pressure to equalize ... @16:40:53.90 SS.reconfigure :exhale @16:40:53.95 SS.hold @16:40:56.82 PTV.dial :puck @16:40:56.84 PSV.dial PTV @16:40:57.62 PBV.dial :waste1 @16:40:58.05 PS.seek 1.5ml @16:41:03.91 delay 3 seconds @16:41:06.93 PBV.dial between PSV and waste1 @16:41:07.31 PS.seek 0ml @16:41:12.19 Intake.select :close @16:41:12.33 SSV.dial :exhaust @16:41:13.10 Exhaust.select :open @16:41:13.21 SS.seek 0ml @16:41:22.41 Exhaust.select :close @16:41:22.52 SSV.dial CBV @16:41:22.53 CBV.dial SSV @16:41:23.30 Allowing 130s for 25.0ml of remaining 25.0ml @16:41:23.31 Intake.select :open @16:41:23.48 delay 10 seconds @16:41:25.47 Intake @16.0psia, vacuum from 10.0 to 13.0psi @16:41:25.49 SS.reconfigure :inhaling 0.43ml/s @16:41:25.53 SS.seek 24.96ml @16:41:33.50 recoverPSRreagent08 @16:41:33.51 PTV.dial :puck @16:41:33.52 PBV.dial :waste1 @16:41:33.88 delay 30 seconds @16:42:03.91 PBV.dial between PSV and waste1 @16:42:04.27 PSR.select :air @16:42:04.29 PSV.dial PSR @16:42:05.06 PS.reconfigure :fast @16:42:05.10 PS.seek 2.5ml @16:42:12.40 Vacuum too high -- waiting up to 40 seconds @16:42:12.41 SS.reconfigure :exhale @16:42:12.46 SS.hold @16:42:19.93 Intake @15.7psia, vacuum from 10.0 to 13.0psi @16:42:19.95 SS.reconfigure :inhaling 0.38ml/s @16:42:19.99 SS.seek 24.96ml @16:42:29.26 PSV.dial PTV @16:42:30.02 delay 5 seconds @16:42:35.04 PSV.dial PSR @16:42:35.80 delay 5 seconds @16:42:37.19 Stroke completed -- waiting for pressure to equalize ... @16:42:37.20 SS.reconfigure :exhale @16:42:37.24 SS.hold @16:42:40.82 PSV.dial PTV @16:42:41.58 delay 5 seconds @16:42:46.60 PSV.dial :air @16:42:47.37 PS.seek 0ml @16:42:51.50 Intake.select :close @16:42:51.64 SSV.dial :exhaust @16:42:52.41 Exhaust.select :open @16:42:52.52 SS.seek 0ml @16:43:01.62 Exhaust.select :close @16:43:01.73 Sampled 1000.0ml @16:43:01.75 Drying puck before fixing for 20:00 @16:43:01.76 CBV.dial :waste2b @16:43:02.51 delay 1:00 @16:43:11.23 Additional air clear @16:43:11.24 PSR.select :air @16:43:11.25 PSV.dial PSR @16:43:12.76 Pulling 2ml of PSR.air @16:43:12.77 PSR.select :air @16:43:12.79 PS.reconfigure :default @16:43:12.84 PS.seek 2ml @16:43:34.16 delay 5 seconds @16:43:39.18 PTV.dial :puck @16:43:39.20 PSV.dial PTV @16:43:39.96 PBV.dial :waste1 @16:43:40.39 PS.seek 1.5ml @16:43:46.29 delay 3 seconds @16:43:49.30 PBV.dial between PSV and waste1 @16:43:49.68 delay 1 second @16:43:50.70 PSV.dial :waste1 @16:43:51.87 PS.seek 0ml @16:44:02.54 CSV.dial :air @16:44:03.00 CS.reconfigure :fast @16:44:03.56 CS.seek 10ml @16:44:08.03 Processing Puck Heater at 27.9C before imaging @16:44:08.04 PH.coast @16:44:08.16 Imaging da puck in Processing Clamp while storing it to tube 3 @16:44:08.32 SC.reconfigure :tight @16:44:08.37 SC.seek 3 @16:44:11.94 SC.configure :limp @16:44:12.13 Elbow.reconfigure :tight @16:44:12.18 Elbow.seek 3 @16:44:13.93 Forearm.reconfigure :tight @16:44:13.98 Forearm.seek 3 + 450 counts @16:44:14.99 SE.reconfigure :pick @16:44:15.04 SE.seek :beamBreak @16:44:17.45 SE.configure :default @16:44:17.60 Elbow.reconfigure :limp @16:44:17.65 Forearm.reconfigure :loose @16:44:17.69 Forearm.seek 3 @16:44:17.93 Elbow.hold @16:44:18.00 Elbow.reconfigure :tight @16:44:18.09 SE.seek 11.11 Pucks below Top Plate @16:44:18.54 Forearm.force -20 @16:44:18.58 Forearm.seek 3 + 313 counts @16:44:18.86 Forearm.reconfigure :tight @16:44:18.91 Forearm.seek 3 + 450 counts @16:44:19.09 SE.seek 12.11 Pucks below Top Plate @16:44:19.62 Forearm.reconfigure :loose @16:44:19.66 Forearm.seek :retract @16:44:20.59 Elbow.seek PC @16:44:23.08 Elbow.reconfigure :loose @16:44:23.16 Forearm.seek PC @16:44:24.23 Hand.close @16:44:24.87 PC.seek :open @16:44:28.33 Pumping wcr puck from tube 4 up to 35psi... @16:44:28.34 CBV.dial SSV @16:44:29.63 Forearm.seek :retract @16:44:29.72 CBV.dial between CSV and waste2b @16:44:30.57 Elbow.reconfigure :tight @16:44:30.63 Elbow.seek 3 @16:44:30.75 CSV.dial between air and CTV @16:44:31.08 CS.seek 7.047ml @16:44:33.27 Forearm.reconfigure :tight @16:44:33.32 Forearm.seek 3 + 450 counts @16:44:34.30 Hand.open @16:44:34.92 SE.seek 11.76 Pucks below Top Plate @16:44:35.19 SE.reconfigure :seal @16:44:35.23 SE.seek 10.8 Pucks below Top Plate @16:44:35.77 Lights, camera, ACTION! @16:44:35.80 PuckCamera: snap -bin=2,2 -auto /var/log/esp/da23mar0116h1000ml.tif @16:44:38.71 CTV.dial CSV,avoiding: :air @16:44:38.73 CSV.dial CTV @16:44:39.56 CS.reconfigure :pressurize @16:44:39.61 CS.seek 0ml @16:44:55.28 Waiting >20 seconds for da to relinquish FlushPuck @16:44:55.87 SX HX9/16 (USB:20): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 16.774 seconds /var/log/esp/da23mar0116h1000ml.tif: TIFF Upload Complete @16:44:55.89 PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da23mar0116h1000ml15s.tif @16:44:57.44 CBV.dial :waste2b @16:44:57.80 CS.reconfigure :default @16:44:57.90 CS.seek 0ml @16:44:59.32 delay 1:00 @16:45:11.51 SX HX9/16 (USB:20): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15 seconds /var/log/esp/midres/da23mar0116h1000ml15s.tif: TIFF Upload Complete @16:45:11.53 PuckCamera: snap -bin=2,2 20.0 /var/log/esp/midres/da23mar0116h1000ml20s.tif @16:45:32.19 SX HX9/16 (USB:20): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 20 seconds /var/log/esp/midres/da23mar0116h1000ml20s.tif: TIFF Upload Complete @16:45:32.20 Gate.power :camera,:OFF @16:45:32.22 DAfans.email "16.774 sec auto-exposure",Subject: "da23mar0116h1000ml images!" @16:45:32.47 Elbow.seek 3 @16:45:32.67 Forearm.seek 3 + 450 counts @16:45:32.73 SE.reconfigure :gently @16:45:32.78 SE.seek 11.51 Pucks below Top Plate @16:45:33.57 Elbow.reconfigure :limp @16:45:33.62 Forearm.reconfigure :loose @16:45:33.68 Forearm.seek 3 @16:45:33.96 Elbow.hold @16:45:34.02 Elbow.reconfigure :tight @16:45:34.08 SE.reconfigure :default @16:45:34.12 SE.seek 11.7 Pucks below Top Plate @16:45:34.50 Forearm.force -20 @16:45:34.54 Forearm.seek 3 + 307 counts @16:45:34.83 Forearm.reconfigure :tight @16:45:34.88 Forearm.seek 3 + 450 counts @16:45:35.17 SE.seek :down @16:45:36.31 Forearm.reconfigure :loose @16:45:36.35 Forearm.seek :retract @16:45:37.11 SE.stop @16:45:37.34 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @16:45:37.53 Elbow.seek FlushPuck::Garage @16:45:39.48 Elbow.reconfigure :loose @16:45:39.69 Forearm.seek :garage @16:45:40.70 Hand.close @16:45:41.61 Forearm.seek :retract @16:45:42.63 Elbow.reconfigure :tight @16:45:42.75 Elbow.seek PC @16:45:44.14 Elbow.reconfigure :loose @16:45:44.34 Forearm.reconfigure :tight @16:45:44.52 Forearm.seek PC @16:45:46.07 PC.seek :closed @16:45:50.89 Processing Clamp onSpring/offSpring for 15/25 samples, on/off torque 1.63 @16:45:51.04 Hand.open @16:45:51.77 Forearm.reconfigure :loose @16:45:51.86 Forearm.seek :retract @16:45:52.87 PSR.select :air @16:45:52.91 PSV.dial PSR @16:45:53.50 Pulling 0.5ml of PSR.flush @16:45:53.52 PSR.select :flush @16:45:53.73 PS.seek 0.5ml @16:45:59.36 CTV.dial :air,avoiding: :intake @16:45:59.68 delay 5 seconds @16:46:00.75 Fix #1 of 2 (with RNAlater) for 10:00 @16:46:00.78 CSV.dial CSR @16:46:01.38 Pulling 2ml of CSR.RNAlater @16:46:01.41 CSR.select :RNAlater @16:46:01.57 CS.seek 2ml @16:46:04.71 PSR.select :air @16:46:04.92 PS.seek 2.5ml @16:46:07.63 delay 5 seconds @16:46:08.62 upsync @16:46:12.66 CSR.select :air @16:46:12.77 CS.reconfigure :fast @16:46:12.82 CS.seek 10ml @16:46:26.16 delay 1 second @16:46:27.18 PRV.dial :waste2,avoiding: :ab2 @16:46:29.05 PTV.dial :PRVmixing @16:46:30.36 PSV.dial :mixloop @16:46:31.52 PS.seek :empty @16:46:32.51 Pumping wcr puck from tube 4 up to 35psi... @16:46:32.53 CBV.dial SSV @16:46:33.83 CBV.dial between CSV and waste2b @16:46:35.26 CSV.dial between air and CTV @16:46:35.88 CS.seek 7.015ml @16:46:43.42 CTV.dial CSV,avoiding: :air @16:46:43.44 CSV.dial CTV @16:46:44.83 CS.reconfigure :pressurize @16:46:44.88 CS.seek 0ml @16:46:58.09 PSR.select :air @16:46:58.11 PSV.dial PSR @16:46:58.59 delay 10:00 @16:46:59.32 Pulling 0.5ml of PSR.flush @16:46:59.33 PSR.select :flush @16:46:59.45 PS.seek 0.5ml @16:47:05.44 delay 5 seconds @16:47:10.46 PSR.select :air @16:47:10.58 PS.seek 2.5ml @16:47:31.78 delay 1 second @16:47:32.80 PBV.dial :waste1 @16:47:33.16 PTV.dial :mixing @16:47:33.91 PSV.dial :mixloop @16:47:35.07 PS.seek :empty @16:48:01.47 PSV.dial PSR @16:48:02.50 PSR.select :flush @16:48:02.61 PS.seek 1ml @16:48:13.65 PSR.select :air @16:48:13.76 PS.seek 2.5ml @16:48:29.97 PSV.dial PTV @16:48:30.73 PTV.dial :puck @16:48:31.47 PBV.dial :waste1 @16:48:31.49 PS.seek 0ml @16:48:57.81 PSV.dial PSR @16:48:58.57 PSR.select :flush @16:48:58.69 PS.seek 0.5ml @16:49:04.65 PSR.select :air @16:49:04.76 PS.seek 2.5ml @16:49:25.96 PSV.dial PTV @16:49:26.73 PBV.dial PSV @16:49:27.30 PS.seek 0ml @16:49:53.84 PSV.dial PBV @16:49:54.32 PS.seek 2.5ml @16:50:20.71 PSV.dial :waste1 @16:50:21.47 PS.seek 0ml @16:50:47.90 PSV.dial PSR @16:50:48.37 PSR.select :air @16:50:48.39 PS.seek 2.5ml @16:51:14.81 PSV.dial PTV @16:51:15.54 PBV.dial PSV @16:51:15.56 PS.seek 0ml @16:51:41.90 PSV.dial PBV @16:51:42.38 PS.seek 2.5ml @16:52:08.68 PSV.dial :waste1 @16:52:09.55 PS.seek 0ml @16:52:35.84 PSV.dial PSR @16:52:36.29 PSR.select :flush @16:52:36.41 PS.seek 1ml @16:52:47.49 PSR.select :air @16:52:47.60 PS.seek 2.5ml @16:53:03.77 PSV.dial PTV @16:53:04.51 PTV.dial :puck @16:53:04.52 PBV.dial :waste1 @16:53:05.08 PS.seek 0ml @16:53:31.65 PSV.dial PSR @16:53:32.38 PSR.select :flush @16:53:32.50 PS.seek 0.5ml @16:53:38.43 PSR.select :air @16:53:38.54 PS.seek 2.5ml @16:53:59.77 PSV.dial PTV @16:54:00.52 PBV.dial PSV @16:54:01.09 PS.seek 0ml @16:54:27.43 PSV.dial PBV @16:54:27.90 PS.seek 2.5ml @16:54:54.21 PSV.dial :waste1 @16:54:54.95 PS.seek 0ml @16:55:21.27 PSV.dial PSR @16:55:21.74 PSR.select :air @16:55:21.76 PS.seek 2.5ml @16:55:48.08 PSV.dial PTV @16:55:48.82 PBV.dial PSV @16:55:48.84 PS.seek 0ml @16:56:15.21 PSV.dial PBV @16:56:15.68 PS.seek 2.5ml @16:56:42.05 PSV.dial :waste1 @16:56:42.86 PS.seek 0ml @16:56:58.61 CBV.dial :waste2b @16:56:58.94 CS.reconfigure :default @16:56:58.99 CS.seek 0ml @16:57:04.59 delay 1:00 @16:57:09.24 Garaging FlushPuck in Processing Clamp @16:57:09.31 Elbow.reconfigure :tight @16:57:09.36 Elbow.seek PC @16:57:09.47 Elbow.reconfigure :loose @16:57:09.57 Forearm.seek PC @16:57:10.56 Hand.close @16:57:11.11 PC.seek :open @16:57:15.85 Forearm.seek :retract @16:57:16.84 Elbow.reconfigure :tight @16:57:16.89 Elbow.seek :garage @16:57:18.24 Elbow.reconfigure :loose @16:57:18.33 Forearm.reconfigure :tight @16:57:18.37 Forearm.seek :garage @16:57:19.43 Hand.open @16:57:20.04 Forearm.reconfigure :loose @16:57:20.08 Forearm.seek :retract @16:58:04.61 CTV.dial :air,avoiding: :intake @16:58:05.91 Fix #2 of 2 (with RNAlater) for 10:00 @16:58:05.92 CSV.dial CSR @16:58:06.41 Pulling 2ml of CSR.RNAlater @16:58:06.42 CSR.select :RNAlater @16:58:06.53 CS.seek 2ml @16:58:12.43 delay 5 seconds @16:58:17.45 CSR.select :air @16:58:17.57 CS.reconfigure :fast @16:58:17.61 CS.seek 10ml @16:58:37.40 Pumping wcr puck from tube 4 up to 35psi... @16:58:37.42 CBV.dial SSV @16:58:38.72 CBV.dial between CSV and waste2b @16:58:39.66 CSV.dial between air and CTV @16:58:40.27 CS.seek 7.008ml @16:58:47.94 CTV.dial CSV,avoiding: :air @16:58:47.96 CSV.dial CTV @16:58:49.34 CS.reconfigure :pressurize @16:58:49.39 CS.seek 0ml @16:59:02.67 delay 10:00 @17:09:02.69 CBV.dial :waste2b @17:09:03.02 CS.reconfigure :default @17:09:03.06 CS.seek 0ml @17:09:08.98 delay 1:00 @17:10:09.01 CTV.dial :air,avoiding: :intake @17:10:10.37 Storing wcr puck in Collection Clamp to tube 3 @17:10:10.53 SC.reconfigure :tight @17:10:10.58 SC.seek 3 @17:10:10.64 SC.configure :limp @17:10:10.84 Elbow.reconfigure :tight @17:10:10.89 Elbow.seek 3 @17:10:12.74 Forearm.reconfigure :tight @17:10:12.78 Forearm.seek 3 + 450 counts @17:10:13.79 SE.reconfigure :pick @17:10:13.85 SE.seek :beamBreak @17:10:16.13 SE.configure :default @17:10:16.28 Elbow.reconfigure :limp @17:10:16.33 Forearm.reconfigure :loose @17:10:16.37 Forearm.seek 3 @17:10:16.61 Elbow.hold @17:10:16.67 Elbow.reconfigure :tight @17:10:16.77 SE.seek 12.14 Pucks below Top Plate @17:10:17.19 Forearm.force -20 @17:10:17.23 Forearm.seek 3 + 300 counts @17:10:17.50 Forearm.reconfigure :tight @17:10:17.55 Forearm.seek 3 + 450 counts @17:10:18.21 SE.seek 13.14 Pucks below Top Plate @17:10:18.77 Forearm.reconfigure :loose @17:10:18.82 Forearm.seek :retract @17:10:19.77 Elbow.seek CC @17:10:21.38 Elbow.reconfigure :loose @17:10:21.47 Forearm.seek CC @17:10:22.45 Hand.close @17:10:23.10 CC.seek :open @17:10:27.82 Forearm.seek :retract @17:10:28.85 Elbow.reconfigure :tight @17:10:28.89 Elbow.seek 3 @17:10:30.67 Elbow.reconfigure :limp @17:10:30.72 Forearm.seek 3 @17:10:31.67 Elbow.hold @17:10:31.74 Elbow.reconfigure :tight @17:10:31.79 Hand.open @17:10:32.45 Forearm.force -20 @17:10:32.49 Forearm.seek 3 + 320 counts @17:10:32.78 Forearm.reconfigure :tight @17:10:32.82 Forearm.seek 3 + 450 counts @17:10:33.08 SE.seek :down @17:10:34.20 Forearm.reconfigure :loose @17:10:34.25 Forearm.seek :retract @17:10:34.90 SE.stop @17:10:35.19
Starting finalPurge @17:10:35.20 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @17:10:35.38 Elbow.seek FlushPuck::Garage @17:10:37.10 Elbow.reconfigure :loose @17:10:37.19 Forearm.seek :garage @17:10:38.20 Hand.close @17:10:38.95 Forearm.seek :retract @17:10:39.91 Elbow.reconfigure :tight @17:10:39.95 Elbow.seek CC @17:10:42.85 Elbow.reconfigure :loose @17:10:42.94 Forearm.reconfigure :tight @17:10:42.98 Forearm.seek CC @17:10:44.14 CC.seek :closed @17:10:48.65 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.35 @17:10:48.73 Hand.open @17:10:49.40 Forearm.reconfigure :loose @17:10:49.44 Forearm.seek :retract @17:10:50.41 Flushing the Collection Valve::Manifold @17:10:50.52 CSV.dial CSR @17:10:51.00 Pulling 5ml of CSR.flush @17:10:51.01 CSR.select :flush @17:10:51.13 CS.seek 5ml @17:11:04.95 delay 5 seconds @17:11:09.97 CSR.select :air @17:11:10.11 CS.reconfigure :fast @17:11:10.16 CS.seek 7.5ml @17:11:16.92 CSV.dial :waste @17:11:18.19 CS.reconfigure :default @17:11:18.23 CS.seek 0ml @17:11:38.26 Flushing lower manifold's waste1 port @17:11:38.38 CSV.dial :flush @17:11:38.81 CS.reconfigure :fast @17:11:38.86 CS.seek 5ml @17:11:51.67 delay 5 seconds @17:11:56.69 CSV.dial :air @17:11:57.18 CS.seek 7.001ml @17:12:02.64 CSV.dial CTV @17:12:02.66
CTV.dial CSV @17:12:04.48 CBV.dial :waste1 @17:12:05.24 CS.seek 0ml @17:12:21.73 Flushing lower manifold's waste2 port @17:12:21.85 CSV.dial :flush @17:12:22.69 CS.seek 5ml @17:12:35.29 delay 5 seconds @17:12:40.31 CSV.dial :air @17:12:40.81 CS.seek 7ml @17:12:46.32 CSV.dial CTV @17:12:46.35
CTV.dial CSV @17:12:46.79 CBV.dial :waste2 @17:12:47.83 CS.seek 0ml @17:13:04.32 Flushing lower manifold's waste2b port @17:13:04.44 CSV.dial :flush @17:13:05.17 CS.seek 5ml @17:13:17.92 delay 5 seconds @17:13:22.93 CSV.dial :air @17:13:23.45 CS.seek 7ml @17:13:28.89 CSV.dial CTV @17:13:28.92
CTV.dial CSV @17:13:29.34 CBV.dial :waste2b @17:13:30.39 CS.seek 0ml @17:13:46.82 Disinfecting external Intake with kill @17:13:46.94 CSV.dial CSR @17:13:47.42 Pulling 8ml of CSR.kill @17:13:47.43 CSR.select :kill @17:13:47.54 CS.seek 8ml @17:14:07.17 delay 5 seconds @17:14:12.18 CSR.select :air @17:14:12.30 CS.seek 10ml @17:14:17.73 CSV.dial CBV @17:14:17.75
CBV.dial CSV @17:14:18.79 CTV.dial :intake @17:14:19.57 Intake.select :open @17:14:19.68 CS.seek 2ml @17:14:38.51 Intake.select :close @17:14:38.61 CTV.dial :air @17:14:39.36 CS.reconfigure :default @17:14:39.40 CS.seek 0ml @17:14:45.29 CSV.dial CSR @17:14:46.33 Pulling 5ml of CSR.flush @17:14:46.34 CSR.select :flush @17:14:46.45 CS.seek 5ml @17:15:00.07 delay 5 seconds @17:15:05.09 CSR.select :air @17:15:05.20 CS.seek 10ml @17:15:18.85 CSV.dial CBV @17:15:19.86 CS.seek 0ml @17:15:46.23 Garaging FlushPuck in Collection Clamp @17:15:46.30 Elbow.reconfigure :tight @17:15:46.34 Elbow.seek CC @17:15:46.73 Elbow.reconfigure :loose @17:15:46.83 Forearm.seek CC @17:15:47.77 Hand.close @17:15:48.34 CC.seek :open @17:15:52.96 Forearm.seek :retract @17:15:53.92 Elbow.reconfigure :tight @17:15:53.96 Elbow.seek :garage @17:15:56.88 Elbow.reconfigure :loose @17:15:56.96 Forearm.reconfigure :tight @17:15:57.00 Forearm.seek :garage @17:15:58.01 Hand.open @17:15:58.65 Forearm.reconfigure :loose @17:15:58.69 Forearm.seek :retract @17:15:59.65 Completed [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] begun 14:34:17 @17:15:59.70 Asleep after completing DA_WCR_030123 assays @17:15:59.72 GoodNews.email "{1 => 33,\n 2 => 33,\n 3 => 14,\n 4 => 19,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@17:13:26, 17% flow, 22.5C, 35% humidity, 14.9psia, 13.695V, 0.466A, 0.453A avg, 6.38W\nSampled 1000.0ml",Subject: "Asleep after completing DA_WCR_030123 assays" @17:16:06.14 upsync @14:26:53.47UTC10-Mar-23
ESP2 v3.41 I2C Gateway v3.20 for core bus @14:26:56.05 Configuration for short puck jake 1/11/21 by brent@mbari.org /home/esp/esp2/type/shallow/jake/configure.rb @14:26:59.96 Polling Can every 10:00 @14:27:00.18 Cleanly exited at 14:23:59UTC10-Mar-23 @14:27:00.22 Loading /home/esp/esp2/mission/DA_WCR_031023.rb mission @14:27:00.28 Preparing to sleep until + 00:00:05 @14:27:00.29 GoodNews.email "Starting DA_WCR_031023 w/pucks in tube 4\n{1 => 33,\n 2 => 33,\n 3 => 14,\n 4 => 19,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@14:26:59, 16% flow, 20.9C, 39% humidity, 14.8psia, 13.734V, 0.284A, 0.266A avg, 3.91W",Subject: "DA_WCR_031023 begun" @14:27:00.33
Asleep til + 00:00:05 @14:27:00.37 Continuing at 14:27 @14:27:00.61 Gate.power :core,:OFF @14:27:00.63 Gate.power :raw,:OFF Shutdown @14:27:04.76 GoodNews.email "Then, in tube 4: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2/mission/DA_WCR_031023.rb:14\n{1 => 33,\n 2 => 33,\n 3 => 14,\n 4 => 19,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@14:27:00, 16% flow, 21.1C, 39% humidity, 14.8psia, 13.734V, 0.284A, 0.266A avg, 3.91W",Subject: "Asleep until 14:27" @14:27:05.28
Powering core bus @14:27:06.58 upsync @14:27:10.02 upsync @14:27:10.85
Gate.power :raw,:ON @14:27:11.70 CS.configure :default @14:27:11.79 PS.configure :default @14:27:11.88 SS.configure :default @14:27:11.97 CC.configure :default @14:27:12.06 PC.configure :default @14:27:12.17 Forearm.configure :loose @14:27:12.26 Elbow.configure :loose @14:27:12.37 SC.configure :loose @14:27:12.46 SE.configure :default @14:27:12.50 CH.coast @14:27:12.55 CH.configure :default @14:27:12.58 PH.coast @14:27:12.63 PH.configure :default @14:27:12.67 All dwarf controllers running firmware version 3.85 @14:27:12.77 Valve.reset @14:27:13.00 Intake.select :close @14:27:13.03 Exhaust.select :close @14:27:13.06 CTV.dial :air @14:27:13.16 PSV.dial :air @14:27:13.26 CSV.dial :waste1 @14:27:13.28
Forearm.reconfigure :loose @14:27:13.36 Forearm.home.seek :retract @14:27:13.75 SSV.dial :spit,avoiding: :exhaust @14:27:14.05 Hand.close @14:27:14.08
Elbow.jog 1500 @14:27:14.24 PS.reconfigure :default @14:27:14.28 PS.home.seek 0ml @14:27:14.50
Elbow.reconfigure :loose @14:27:14.57 Elbow.home.seek :clear @14:27:14.61 CS.reconfigure :fast @14:27:14.63 SS.reconfigure :default @14:27:14.76 SS.home.seek 0ml @14:27:14.77 CS.home.seek 0ml @14:27:17.90
SE.reconfigure :default @14:27:17.95 SE.home.seek :down @14:27:18.55 SC.reconfigure :tight @14:27:18.59 SC.home.seek :initial @14:27:22.53 PS.stop @14:27:46.85
SC.configure :limp @14:27:46.90 Forearm.seek :retract @14:27:46.96 Elbow.seek :hammer @14:27:48.00 Forearm.reconfigure :tight @14:27:48.05 Forearm.seek retracted @14:27:48.27 CC.reconfigure :default @14:27:48.28 PC.reconfigure :default @14:27:48.37
CC.home.seek :open @14:27:48.39 PC.home.seek :open @14:27:51.92
Phase [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] @14:27:51.93 Notice.email "At 14:27:51 began in tube 4: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2/mission/DA_WCR_031023.rb:14\n{1 => 33,\n 2 => 33,\n 3 => 14,\n 4 => 19,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@14:27:51, 16% flow, 21.1C, 39% humidity, 14.8psia, 13.666V, 0.321A, 0.302A avg, 4.38W",Subject: "Started DA_WCR_031023 phase initialPurge; da [1000, 1000] #da and wsr are run simultanelously" @14:27:51.95
Starting initialPurge @14:27:51.97 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:27:52.10 Hand.open @14:27:52.85 Elbow.reconfigure :tight @14:27:52.90 Elbow.seek FlushPuck::Garage @14:27:54.76 Elbow.reconfigure :loose @14:27:54.87 Forearm.reconfigure :loose @14:27:54.94 Forearm.seek :garage @14:27:55.98 Hand.close @14:27:56.73 Forearm.seek :retract @14:27:57.71 Elbow.reconfigure :tight @14:27:57.75 Elbow.seek CC @14:27:57.77 upsync @14:28:00.70
Elbow.reconfigure :loose @14:28:00.79 Forearm.reconfigure :tight @14:28:00.84 Forearm.seek CC @14:28:02.02 CC.seek :closed @14:28:06.54 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.33 @14:28:06.60 Hand.open @14:28:07.29 Forearm.reconfigure :loose @14:28:07.33 Forearm.seek :retract @14:28:08.27 Flushing the Collection Valve::Manifold @14:28:08.39 CSV.dial CSR @14:28:09.73 Pulling 5ml of CSR.flush CSR.select :flush @14:28:09.84 CS.reconfigure :default @14:28:09.89 CS.seek 5ml @14:28:23.58 delay 5 seconds @14:28:28.60 CSR.select :air @14:28:28.74 CS.reconfigure :fast @14:28:28.79 CS.seek 7.5ml @14:28:35.54 CSV.dial :waste @14:28:36.81 CS.reconfigure :default @14:28:36.86 CS.seek 0ml @14:28:56.86 Purging external Intake @14:28:56.97 CSV.dial :flush @14:28:57.41 CS.reconfigure :fast @14:28:57.45 CS.seek 10ml @14:29:21.98 delay 5 seconds @14:29:27.00 CSV.dial CBV @14:29:27.03
CBV.dial CSV @14:29:27.78 CTV.dial :intake @14:29:28.56 Intake.select :open @14:29:28.66 CS.seek 0ml @14:29:52.20 Intake.select :close @14:29:52.30 CTV.dial :air @14:29:53.09 Flushing upper manifold @14:29:53.21 CSV.dial :flush @14:29:53.94 CS.seek 5ml @14:30:06.64 delay 5 seconds @14:30:11.65 CSV.dial :air @14:30:12.15 CS.seek 7.001ml @14:30:17.73 CSV.dial CBV @14:30:17.75
CBV.dial CSV @14:30:18.78 CTV.dial :air @14:30:18.80 CS.seek 0ml @14:30:35.42 delay 7 seconds @14:30:42.44 CS.seek 3ml @14:30:50.45 CSV.dial :waste @14:30:50.89 CS.seek 0ml @14:30:58.62 CSV.dial :flush @14:30:59.05 CS.seek 3ml @14:31:07.01 delay 5 seconds @14:31:12.03 CSV.dial :air @14:31:12.52 CS.seek 5ml @14:31:18.01 CSV.dial :air @14:31:18.03 CS.seek 0ml @14:31:30.10 delay 7 seconds @14:31:37.13 CS.seek 3ml @14:31:45.20 CSV.dial :waste @14:31:45.92 CS.seek 0ml @14:31:53.67 Garaging FlushPuck in Collection Clamp @14:31:53.73 Elbow.reconfigure :tight @14:31:53.78 Elbow.seek CC @14:31:54.03 Elbow.reconfigure :loose @14:31:54.12 Forearm.seek CC @14:31:55.12 Hand.close @14:31:55.72 CC.seek :open @14:32:00.34 Forearm.seek :retract @14:32:01.28 Elbow.reconfigure :tight @14:32:01.32 Elbow.seek :garage @14:32:04.32 Elbow.reconfigure :loose @14:32:04.41 Forearm.reconfigure :tight @14:32:04.45 Forearm.seek :garage @14:32:05.46 Hand.open @14:32:06.22 Forearm.reconfigure :loose @14:32:06.27 Forearm.seek :retract @14:32:07.23 da sampling at most 1000ml, wcr at most 1000ml @14:32:07.27 Loading sh1 puck from tube 4 into Collection Clamp @14:32:07.28 DAfans.email "Started da in tube 4 at 14:32:07UTC10-Mar-23\n{1 => 33,\n 2 => 33,\n 3 => 14,\n 4 => 19,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@14:27:51, 16% flow, 21.1C, 39% humidity, 14.8psia, 13.666V, 0.321A, 0.302A avg, 4.38W",Subject: "da sampling at most 1000ml, wcr at most 1000ml" @14:32:07.76
Elbow.reconfigure :tight @14:32:07.81 Elbow.seek 4 @14:32:09.78 Forearm.reconfigure :tight @14:32:09.82 Forearm.seek 4 + 450 counts @14:32:11.00 SE.reconfigure :pick @14:32:11.08 SE.seek :beamBreak @14:32:12.58 upsync @14:32:13.00
SE.configure :default @14:32:13.15 Elbow.seek 4 @14:32:13.33 Forearm.seek 4 + 300 counts @14:32:13.51 Forearm.reconfigure :loose @14:32:13.56 Hand.close @14:32:14.23 SE.seek :down @14:32:14.42
Forearm.seek :retract @14:32:15.37 Elbow.seek CC @14:32:15.82 SE.stop @14:32:17.01
Elbow.reconfigure :loose @14:32:17.10 Forearm.reconfigure :tight @14:32:17.14 Forearm.seek CC @14:32:18.42 CC.seek :closed @14:32:22.99 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.21 @14:32:23.00 Hand.open @14:32:23.65 Forearm.reconfigure :loose @14:32:23.69 Forearm.seek :retract @14:32:24.66 collectSample 1000ml w/bubble point of 24psi @14:32:24.67 Priming 7ml @14:32:24.68 Intake.select :close @14:32:24.69 Exhaust.select :close @14:32:24.70 SS.hold @14:32:24.89 Calibrating pressure and temperature sensors @14:32:24.92 CTV.dial :air @14:32:24.94 SSV.dial :spit,avoiding: :exhaust @14:32:24.95 SS.reconfigure :fast @14:32:25.00 SS.seek 5ml @14:32:27.83 SSV.dial CBV @14:32:27.86
CBV.dial SSV @14:32:29.18 delay 3.8 seconds @14:32:33.13 SSV.dial :spit @14:32:34.43 SS.seek 0ml @14:32:37.18 SSV.dial CBV @14:32:38.47 CTV.dial :intake @14:32:39.26 SSV.dial CBV @14:32:39.28
CBV.dial SSV @14:32:39.30 Allowing 40s for 7.0ml of remaining 7.0ml @14:32:39.31 Intake.select :open @14:32:41.46 Intake @15.2psia, vacuum from 9.7 to 12.7psi @14:32:41.48 SS.reconfigure :inhaling 2.20ml/s @14:32:41.52 SS.seek 7ml @14:32:48.35 Stroke completed -- waiting for pressure to equalize ... @14:32:48.36 SS.reconfigure :exhale @14:32:48.40 SS.hold @14:32:48.70 Intake.select :close @14:32:48.84 SSV.dial :exhaust @14:32:49.58 Exhaust.select :open @14:32:49.69 SS.seek 0ml @14:32:53.55 Exhaust.select :close @14:32:53.65 Sampling 1000.0ml @14:32:53.66 Intake.select :close @14:32:53.67 Exhaust.select :close @14:32:53.68 SS.reconfigure :default @14:32:53.72 SS.hold @14:32:53.90 CTV.dial :intake @14:32:53.92 SSV.dial CBV @14:32:53.94
CBV.dial SSV @14:32:54.71 Allowing 130s for 25.0ml of remaining 1000.0ml @14:32:54.72 Intake.select :open @14:32:56.87 Intake @14.8psia, vacuum from 9.3 to 12.3psi @14:32:56.89 SS.reconfigure :inhaling 2.20ml/s @14:32:56.93 SS.seek 25ml @14:33:10.20 Vacuum too high -- waiting up to 40 seconds @14:33:10.21 SS.reconfigure :exhale @14:33:10.25 SS.hold @14:33:10.95 Intake @12.0psia, vacuum from 6.5 to 9.5psi @14:33:10.97 SS.reconfigure :inhaling 1.98ml/s @14:33:11.01 SS.seek 25ml @14:33:14.08 Stroke completed -- waiting for pressure to equalize ... @14:33:14.09 SS.reconfigure :exhale @14:33:14.13 SS.hold @14:33:19.38 Intake.select :close @14:33:19.52 SSV.dial :exhaust @14:33:20.25 Exhaust.select :open @14:33:20.36 SS.seek 0ml @14:33:29.09 Exhaust.select :close @14:33:29.20 SSV.dial CBV @14:33:29.22
CBV.dial SSV @14:33:29.97 Allowing 130s for 25.0ml of remaining 975.0ml @14:33:29.98 Intake.select :open @14:33:32.14 Intake @15.6psia, vacuum from 10.0 to 13.0psi @14:33:32.16 SS.reconfigure :inhaling 1.98ml/s @14:33:32.20 SS.seek 25ml @14:33:47.57 Vacuum too high -- waiting up to 40 seconds @14:33:47.58 SS.reconfigure :exhale @14:33:47.62 SS.hold @14:33:48.27 Intake @12.7psia, vacuum from 7.2 to 10.2psi @14:33:48.28 SS.reconfigure :inhaling 1.79ml/s @14:33:48.33 SS.seek 25ml @14:33:49.50 Stroke completed -- waiting for pressure to equalize ... @14:33:49.51 SS.reconfigure :exhale @14:33:49.55 SS.hold @14:33:57.05 Intake.select :close @14:33:57.18 SSV.dial :exhaust @14:33:57.92 Exhaust.select :open @14:33:58.03 SS.seek 0ml @14:34:07.13 Exhaust.select :close @14:34:07.23 SSV.dial CBV @14:34:07.25
CBV.dial SSV @14:34:08.00 Allowing 130s for 25.0ml of remaining 950.0ml @14:34:08.01 Intake.select :open @14:34:10.17 Intake @15.7psia, vacuum from 10.0 to 13.0psi @14:34:10.19 SS.reconfigure :inhaling 1.79ml/s @14:34:10.23 SS.seek 25ml @14:34:27.47 Stroke completed -- waiting for pressure to equalize ... @14:34:27.48 SS.reconfigure :exhale @14:34:27.52 SS.hold @14:34:34.92 Intake.select :close @14:34:35.05 SSV.dial :exhaust @14:34:35.80 Exhaust.select :open @14:34:35.90 SS.seek 0ml @14:34:44.73 Exhaust.select :close @14:34:44.84 SSV.dial CBV @14:34:44.87
CBV.dial SSV @14:34:45.60 Allowing 130s for 25.0ml of remaining 925.0ml @14:34:45.61 Intake.select :open @14:34:47.76 Intake @15.8psia, vacuum from 10.0 to 13.0psi @14:34:47.78 SS.reconfigure :inhaling 1.79ml/s @14:34:47.82 SS.seek 25ml @14:35:04.70 Vacuum too high -- waiting up to 40 seconds SS.reconfigure :exhale @14:35:04.74 SS.hold @14:35:05.13 Intake @13.0psia, vacuum from 7.5 to 10.5psi @14:35:05.15 SS.reconfigure :inhaling 1.61ml/s @14:35:05.19 SS.seek 25ml @14:35:05.66 Stroke completed -- waiting for pressure to equalize ... @14:35:05.67 SS.reconfigure :exhale @14:35:05.71 SS.hold @14:35:13.10 Intake.select :close @14:35:13.24 SSV.dial :exhaust @14:35:13.98 Exhaust.select :open @14:35:14.09 SS.seek 0ml @14:35:22.95 Exhaust.select :close @14:35:23.05 SSV.dial CBV @14:35:23.10
CBV.dial SSV @14:35:23.81 Allowing 130s for 25.0ml of remaining 900.0ml @14:35:23.82 Intake.select :open @14:35:25.98 Intake @15.7psia, vacuum from 10.0 to 13.0psi @14:35:25.99 SS.reconfigure :inhaling 1.61ml/s @14:35:26.04 SS.seek 25ml @14:35:44.75 Stroke completed -- waiting for pressure to equalize ... @14:35:44.76 SS.reconfigure :exhale @14:35:44.80 SS.hold @14:35:52.18 Intake.select :close @14:35:52.32 SSV.dial :exhaust @14:35:53.06 Exhaust.select :open @14:35:53.17 SS.seek 0ml @14:36:01.87 Exhaust.select :close @14:36:02.47 SSV.dial CBV @14:36:02.48
CBV.dial SSV @14:36:03.25 Allowing 130s for 25.0ml of remaining 875.0ml @14:36:03.26 Intake.select :open @14:36:05.41 Intake @15.8psia, vacuum from 10.0 to 13.0psi @14:36:05.43 SS.reconfigure :inhaling 1.61ml/s @14:36:05.47 SS.seek 25ml @14:36:24.47 Stroke completed -- waiting for pressure to equalize ... @14:36:24.48 SS.reconfigure :exhale @14:36:24.52 SS.hold @14:36:32.99 Intake.select :close @14:36:33.13 SSV.dial :exhaust @14:36:33.89 Exhaust.select :open @14:36:34.02 SS.seek 0ml @14:36:43.01 Exhaust.select :close @14:36:43.12 SSV.dial CBV @14:36:43.16
CBV.dial SSV @14:36:43.89 Allowing 130s for 25.0ml of remaining 850.0ml @14:36:43.90 Intake.select :open @14:36:46.06 Intake @15.9psia, vacuum from 10.0 to 13.0psi @14:36:46.07 SS.reconfigure :inhaling 1.61ml/s @14:36:46.11 SS.seek 25ml @14:37:03.32 Vacuum too high -- waiting up to 40 seconds @14:37:03.33 SS.reconfigure :exhale @14:37:03.37 SS.hold @14:37:04.81 Intake @13.1psia, vacuum from 7.6 to 10.6psi @14:37:04.82 SS.reconfigure :inhaling 1.45ml/s @14:37:04.86 SS.seek 25ml @14:37:06.48 Stroke completed -- waiting for pressure to equalize ... @14:37:06.49 SS.reconfigure :exhale @14:37:06.53 SS.hold @14:37:12.95 Intake.select :close @14:37:13.08 SSV.dial :exhaust @14:37:13.84 Exhaust.select :open @14:37:13.95 SS.seek 0ml @14:37:22.81 Exhaust.select :close @14:37:22.91 SSV.dial CBV @14:37:22.93
CBV.dial SSV @14:37:23.70 Allowing 130s for 25.0ml of remaining 825.0ml @14:37:23.71 Intake.select :open @14:37:25.86 Intake @15.8psia, vacuum from 10.0 to 13.0psi @14:37:25.88 SS.reconfigure :inhaling 1.45ml/s @14:37:25.92 SS.seek 25ml @14:37:45.82 Stroke completed -- waiting for pressure to equalize ... @14:37:45.83 SS.reconfigure :exhale @14:37:45.87 SS.hold @14:37:53.25 Intake.select :close @14:37:53.39 SSV.dial :exhaust @14:37:54.14 Exhaust.select :open @14:37:54.24 SS.seek 0ml @14:38:03.26 Exhaust.select :close @14:38:03.37 SSV.dial CBV @14:38:03.39
CBV.dial SSV @14:38:04.15 Allowing 130s for 25.0ml of remaining 800.0ml @14:38:04.16 Intake.select :open @14:38:06.32 Intake @15.7psia, vacuum from 10.0 to 13.0psi @14:38:06.33 SS.reconfigure :inhaling 1.45ml/s @14:38:06.37 SS.seek 25ml @14:38:26.46 Stroke completed -- waiting for pressure to equalize ... @14:38:26.47 SS.reconfigure :exhale @14:38:26.51 SS.hold @14:38:33.90 Intake.select :close @14:38:34.03 SSV.dial :exhaust @14:38:34.79 Exhaust.select :open @14:38:34.90 SS.seek 0ml @14:38:43.99 Exhaust.select :close @14:38:44.10 SSV.dial CBV @14:38:44.13
CBV.dial SSV @14:38:44.87 Allowing 130s for 25.0ml of remaining 775.0ml @14:38:44.88 Intake.select :open @14:38:47.04 Intake @15.7psia, vacuum from 10.0 to 13.0psi @14:38:47.05 SS.reconfigure :inhaling 1.45ml/s @14:38:47.09 SS.seek 25ml @14:39:06.81 Stroke completed -- waiting for pressure to equalize ... @14:39:06.82 SS.reconfigure :exhale @14:39:06.86 SS.hold @14:39:14.72 Intake.select :close @14:39:14.86 SSV.dial :exhaust @14:39:15.60 Exhaust.select :open @14:39:15.71 SS.seek 0ml @14:39:24.56 Exhaust.select :close @14:39:24.66 SSV.dial CBV @14:39:24.68
CBV.dial SSV @14:39:25.43 Allowing 130s for 25.0ml of remaining 750.0ml @14:39:25.44 Intake.select :open @14:39:27.60 Intake @15.7psia, vacuum from 10.0 to 13.0psi @14:39:27.62 SS.reconfigure :inhaling 1.45ml/s @14:39:27.66 SS.seek 25ml @14:39:47.82 Vacuum too high -- waiting up to 40 seconds @14:39:47.83 SS.reconfigure :exhale @14:39:47.87 SS.hold @14:39:48.16 Intake @13.3psia, vacuum from 7.8 to 10.8psi @14:39:48.17 SS.reconfigure :inhaling 1.30ml/s @14:39:48.22 SS.seek 25ml @14:39:48.51 Stroke completed -- waiting for pressure to equalize ... @14:39:48.52 SS.reconfigure :exhale @14:39:48.56 SS.hold @14:39:56.41 Intake.select :close @14:39:56.55 SSV.dial :exhaust @14:39:57.29 Exhaust.select :open @14:39:57.40 SS.seek 0ml @14:40:06.12 Exhaust.select :close @14:40:06.22 SSV.dial CBV @14:40:06.24
CBV.dial SSV @14:40:07.00 Allowing 130s for 25.0ml of remaining 725.0ml @14:40:07.01 Intake.select :open @14:40:09.16 Intake @15.6psia, vacuum from 10.0 to 13.0psi @14:40:09.17 SS.reconfigure :inhaling 1.30ml/s @14:40:09.22 SS.seek 25ml @14:40:31.52 Stroke completed -- waiting for pressure to equalize ... @14:40:31.53 SS.reconfigure :exhale @14:40:31.57 SS.hold @14:40:38.95 Intake.select :close @14:40:39.08 SSV.dial :exhaust @14:40:39.82 Exhaust.select :open @14:40:39.93 SS.seek 0ml @14:40:48.68 Exhaust.select :close @14:40:48.78 SSV.dial CBV @14:40:48.80
CBV.dial SSV @14:40:49.56 Allowing 130s for 25.0ml of remaining 700.0ml @14:40:49.57 Intake.select :open @14:40:51.72 Intake @15.7psia, vacuum from 10.0 to 13.0psi @14:40:51.74 SS.reconfigure :inhaling 1.30ml/s @14:40:51.78 SS.seek 25ml @14:41:13.96 Stroke completed -- waiting for pressure to equalize ... @14:41:13.97 SS.reconfigure :exhale @14:41:14.01 SS.hold @14:41:22.50 Intake.select :close @14:41:22.63 SSV.dial :exhaust @14:41:23.37 Exhaust.select :open @14:41:23.47 SS.seek 0ml @14:41:32.52 Exhaust.select :close @14:41:32.63 SSV.dial CBV @14:41:32.65
CBV.dial SSV @14:41:33.40 Allowing 130s for 25.0ml of remaining 675.0ml @14:41:33.41 Intake.select :open @14:41:35.56 Intake @15.8psia, vacuum from 10.0 to 13.0psi @14:41:35.58 SS.reconfigure :inhaling 1.30ml/s @14:41:35.62 SS.seek 25ml @14:41:57.10 Stroke completed -- waiting for pressure to equalize ... @14:41:57.11 SS.reconfigure :exhale @14:41:57.15 SS.hold @14:42:05.61 Intake.select :close @14:42:05.74 SSV.dial :exhaust @14:42:06.48 Exhaust.select :open @14:42:06.59 SS.seek 0ml @14:42:15.40 Exhaust.select :close @14:42:15.50 SSV.dial CBV @14:42:15.52
CBV.dial SSV @14:42:16.76 Allowing 130s for 25.0ml of remaining 650.0ml @14:42:16.77 Intake.select :open @14:42:18.92 Intake @15.9psia, vacuum from 10.0 to 13.0psi @14:42:18.94 SS.reconfigure :inhaling 1.30ml/s @14:42:18.98 SS.seek 25ml @14:42:38.59 Vacuum too high -- waiting up to 40 seconds @14:42:38.60 SS.reconfigure :exhale @14:42:38.64 SS.hold @14:42:41.74 Intake @14.0psia, vacuum from 8.5 to 11.5psi @14:42:41.76 SS.reconfigure :inhaling 1.17ml/s @14:42:41.80 SS.seek 25ml @14:42:45.25 Stroke completed -- waiting for pressure to equalize ... @14:42:45.26 SS.reconfigure :exhale @14:42:45.31 SS.hold @14:42:50.10 Intake.select :close @14:42:50.23 SSV.dial :exhaust @14:42:50.98 Exhaust.select :open @14:42:51.08 SS.seek 0ml @14:42:59.90 Exhaust.select :close @14:43:00.00 SSV.dial CBV @14:43:00.02
CBV.dial SSV @14:43:00.79 Allowing 130s for 25.0ml of remaining 625.0ml @14:43:00.80 Intake.select :open @14:43:02.96 Intake @15.9psia, vacuum from 10.0 to 13.0psi @14:43:02.97 SS.reconfigure :inhaling 1.17ml/s @14:43:03.01 SS.seek 25ml @14:43:27.35 Stroke completed -- waiting for pressure to equalize ... @14:43:27.36 SS.reconfigure :exhale @14:43:27.40 SS.hold @14:43:35.85 Intake.select :close @14:43:35.98 SSV.dial :exhaust @14:43:36.72 Exhaust.select :open @14:43:36.83 SS.seek 0ml @14:43:45.82 Exhaust.select :close @14:43:45.92 SSV.dial CBV @14:43:45.94
CBV.dial SSV @14:43:46.69 Allowing 130s for 25.0ml of remaining 600.0ml @14:43:46.70 Intake.select :open @14:43:48.86 Intake @16.0psia, vacuum from 10.0 to 13.0psi @14:43:48.88 SS.reconfigure :inhaling 1.17ml/s @14:43:48.92 SS.seek 25ml @14:44:12.89 Stroke completed -- waiting for pressure to equalize ... @14:44:12.90 SS.reconfigure :exhale @14:44:12.94 SS.hold @14:44:21.51 Intake.select :close @14:44:21.65 SSV.dial :exhaust @14:44:22.39 Exhaust.select :open @14:44:22.50 SS.seek 0ml @14:44:31.33 Exhaust.select :close @14:44:31.43 SSV.dial CBV @14:44:31.46
CBV.dial SSV @14:44:32.21 Allowing 130s for 25.0ml of remaining 575.0ml @14:44:32.22 Intake.select :open @14:44:34.37 Intake @15.9psia, vacuum from 10.0 to 13.0psi @14:44:34.39 SS.reconfigure :inhaling 1.17ml/s @14:44:34.43 SS.seek 25ml @14:44:58.71 Stroke completed -- waiting for pressure to equalize ... @14:44:58.72 SS.reconfigure :exhale @14:44:58.76 SS.hold @14:45:07.21 Intake.select :close @14:45:07.35 SSV.dial :exhaust @14:45:08.10 Exhaust.select :open @14:45:08.20 SS.seek 0ml @14:45:16.99 Exhaust.select :close @14:45:17.09 SSV.dial CBV @14:45:17.11
CBV.dial SSV @14:45:17.86 Allowing 130s for 25.0ml of remaining 550.0ml @14:45:17.87 Intake.select :open @14:45:20.50 Intake @15.9psia, vacuum from 10.0 to 13.0psi @14:45:20.52 SS.reconfigure :inhaling 1.17ml/s @14:45:20.56 SS.seek 25ml @14:45:43.34 Vacuum too high -- waiting up to 40 seconds @14:45:43.35 SS.reconfigure :exhale @14:45:43.39 SS.hold @14:45:46.40 Intake @14.0psia, vacuum from 8.5 to 11.5psi @14:45:46.41 SS.reconfigure :inhaling 1.06ml/s @14:45:46.45 SS.seek 25ml @14:45:48.43 Stroke completed -- waiting for pressure to equalize ... @14:45:48.44 SS.reconfigure :exhale @14:45:48.48 SS.hold @14:45:54.06 Intake.select :close @14:45:54.20 SSV.dial :exhaust @14:45:54.94 Exhaust.select :open @14:45:55.05 SS.seek 0ml @14:46:04.03 Exhaust.select :close @14:46:04.14 SSV.dial CBV @14:46:04.19
CBV.dial SSV @14:46:04.89 Allowing 130s for 25.0ml of remaining 525.0ml @14:46:04.91 Intake.select :open @14:46:07.06 Intake @15.9psia, vacuum from 10.0 to 13.0psi @14:46:07.07 SS.reconfigure :inhaling 1.06ml/s @14:46:07.12 SS.seek 25ml @14:46:33.08 Stroke completed -- waiting for pressure to equalize ... @14:46:33.09 SS.reconfigure :exhale @14:46:33.13 SS.hold @14:46:41.60 Intake.select :close @14:46:41.74 SSV.dial :exhaust @14:46:42.49 Exhaust.select :open @14:46:42.59 SS.seek 0ml @14:46:51.34 Exhaust.select :close @14:46:51.45 SSV.dial CBV @14:46:51.47
CBV.dial SSV @14:46:52.22 Allowing 130s for 25.0ml of remaining 500.0ml @14:46:52.23 Intake.select :open @14:46:54.38 Intake @15.8psia, vacuum from 10.0 to 13.0psi @14:46:54.40 SS.reconfigure :inhaling 1.06ml/s @14:46:54.44 SS.seek 25ml @14:47:20.98 Stroke completed -- waiting for pressure to equalize ... @14:47:20.99 SS.reconfigure :exhale @14:47:21.03 SS.hold @14:47:29.50 Intake.select :close @14:47:29.63 SSV.dial :exhaust @14:47:30.38 Exhaust.select :open @14:47:30.48 SS.seek 0ml @14:47:39.36 Exhaust.select :close @14:47:39.46 SSV.dial CBV @14:47:39.48
CBV.dial SSV @14:47:40.25 Allowing 130s for 25.0ml of remaining 475.0ml @14:47:40.26 Intake.select :open @14:47:42.42 Intake @16.0psia, vacuum from 10.0 to 13.0psi @14:47:42.43 SS.reconfigure :inhaling 1.06ml/s @14:47:42.48 SS.seek 25ml @14:48:08.90 Stroke completed -- waiting for pressure to equalize ... @14:48:08.91 SS.reconfigure :exhale @14:48:08.96 SS.hold @14:48:17.97 Intake.select :close @14:48:18.10 SSV.dial :exhaust @14:48:18.85 Exhaust.select :open @14:48:18.95 SS.seek 0ml @14:48:27.75 Exhaust.select :close @14:48:27.85 SSV.dial CBV @14:48:27.88
CBV.dial SSV @14:48:28.63 Allowing 130s for 25.0ml of remaining 450.0ml @14:48:28.64 Intake.select :open @14:48:30.79 Intake @16.0psia, vacuum from 10.0 to 13.0psi @14:48:30.81 SS.reconfigure :inhaling 1.06ml/s @14:48:30.85 SS.seek 25ml @14:48:53.34 Vacuum too high -- waiting up to 40 seconds @14:48:53.35 SS.reconfigure :exhale @14:48:53.39 SS.hold @14:48:56.94 Intake @14.5psia, vacuum from 9.0 to 12.0psi @14:48:56.96 SS.reconfigure :inhaling 0.95ml/s @14:48:57.00 SS.seek 25ml @14:49:02.01 Stroke completed -- waiting for pressure to equalize ... @14:49:02.02 SS.reconfigure :exhale @14:49:02.06 SS.hold @14:49:07.65 Intake.select :close @14:49:07.78 SSV.dial :exhaust @14:49:08.53 Exhaust.select :open @14:49:08.64 SS.seek 0ml @14:49:17.31 Exhaust.select :close @14:49:17.41 SSV.dial CBV @14:49:17.43
CBV.dial SSV @14:49:18.19 Allowing 130s for 25.0ml of remaining 425.0ml @14:49:18.20 Intake.select :open @14:49:20.35 Intake @16.0psia, vacuum from 10.0 to 13.0psi @14:49:20.37 SS.reconfigure :inhaling 0.95ml/s @14:49:20.43 SS.seek 25ml @14:49:49.42 Stroke completed -- waiting for pressure to equalize ... @14:49:49.43 SS.reconfigure :exhale @14:49:49.47 SS.hold @14:49:57.93 Intake.select :close @14:49:58.07 SSV.dial :exhaust @14:49:58.81 Exhaust.select :open @14:49:58.92 SS.seek 0ml @14:50:07.75 Exhaust.select :close @14:50:07.85 SSV.dial CBV @14:50:07.87
CBV.dial SSV @14:50:08.62 Allowing 130s for 25.0ml of remaining 400.0ml @14:50:08.63 Intake.select :open @14:50:10.79 Intake @16.0psia, vacuum from 10.0 to 13.0psi @14:50:10.81 SS.reconfigure :inhaling 0.95ml/s @14:50:10.85 SS.seek 25ml @14:50:39.87 Stroke completed -- waiting for pressure to equalize ... @14:50:39.88 SS.reconfigure :exhale @14:50:39.92 SS.hold @14:50:49.59 Intake.select :close @14:50:49.72 SSV.dial :exhaust @14:50:50.47 Exhaust.select :open @14:50:50.57 SS.seek 0ml @14:50:59.51 Exhaust.select :close @14:50:59.61 SSV.dial CBV @14:50:59.63
CBV.dial SSV @14:51:00.39 Allowing 130s for 25.0ml of remaining 375.0ml @14:51:00.40 Intake.select :open @14:51:02.55 Intake @16.0psia, vacuum from 10.0 to 13.0psi @14:51:02.57 SS.reconfigure :inhaling 0.95ml/s @14:51:02.61 SS.seek 25ml @14:51:31.12 Stroke completed -- waiting for pressure to equalize ... @14:51:31.13 SS.reconfigure :exhale @14:51:31.63 SS.hold @14:51:41.43 Intake.select :close @14:51:41.57 SSV.dial :exhaust @14:51:42.31 Exhaust.select :open @14:51:42.42 SS.seek 0ml @14:51:51.20 Exhaust.select :close @14:51:51.30 SSV.dial CBV @14:51:51.32
CBV.dial SSV @14:51:52.07 Allowing 130s for 25.0ml of remaining 350.0ml @14:51:52.09 Intake.select :open @14:51:54.24 Intake @16.0psia, vacuum from 10.0 to 13.0psi @14:51:54.26 SS.reconfigure :inhaling 0.95ml/s @14:51:54.30 SS.seek 25ml @14:52:21.52 Vacuum too high -- waiting up to 40 seconds @14:52:21.53 SS.reconfigure :exhale @14:52:21.57 SS.hold @14:52:25.73 Intake @14.5psia, vacuum from 9.0 to 12.0psi @14:52:25.74 SS.reconfigure :inhaling 0.87ml/s @14:52:25.79 SS.seek 25ml @14:52:28.01 Stroke completed -- waiting for pressure to equalize ... @14:52:28.02 SS.reconfigure :exhale @14:52:28.06 SS.hold @14:52:33.73 Intake.select :close @14:52:33.87 SSV.dial :exhaust @14:52:34.62 Exhaust.select :open @14:52:34.73 SS.seek 0ml @14:52:43.79 Exhaust.select :close @14:52:43.89 SSV.dial CBV @14:52:43.92
CBV.dial SSV @14:52:44.67 Allowing 130s for 25.0ml of remaining 325.0ml @14:52:44.68 Intake.select :open @14:52:46.83 Intake @16.0psia, vacuum from 10.0 to 13.0psi @14:52:46.85 SS.reconfigure :inhaling 0.87ml/s @14:52:46.89 SS.seek 25ml @14:53:18.04 Stroke completed -- waiting for pressure to equalize ... @14:53:18.05 SS.reconfigure :exhale @14:53:18.09 SS.hold @14:53:27.79 Intake.select :close @14:53:27.92 SSV.dial :exhaust @14:53:28.67 Exhaust.select :open @14:53:28.78 SS.seek 0ml @14:53:37.55 Exhaust.select :close @14:53:37.66 SSV.dial CBV @14:53:37.69
CBV.dial SSV @14:53:38.45 Allowing 130s for 25.0ml of remaining 300.0ml @14:53:38.46 Intake.select :open @14:53:40.61 Intake @16.0psia, vacuum from 10.0 to 13.0psi @14:53:40.63 SS.reconfigure :inhaling 0.87ml/s @14:53:40.67 SS.seek 25ml @14:54:12.29 Stroke completed -- waiting for pressure to equalize ... @14:54:12.30 SS.reconfigure :exhale @14:54:12.34 SS.hold @14:54:22.04 Intake.select :close @14:54:22.18 SSV.dial :exhaust @14:54:22.93 Exhaust.select :open @14:54:23.04 SS.seek 0ml @14:54:32.04 Exhaust.select :close @14:54:32.14 SSV.dial CBV @14:54:32.16
CBV.dial SSV @14:54:32.93 Allowing 130s for 25.0ml of remaining 275.0ml @14:54:32.94 Intake.select :open @14:54:35.10 Intake @16.1psia, vacuum from 10.0 to 13.0psi @14:54:35.12 SS.reconfigure :inhaling 0.87ml/s @14:54:35.16 SS.seek 25ml @14:55:05.76 Vacuum too high -- waiting up to 40 seconds @14:55:05.77 SS.reconfigure :exhale @14:55:05.82 SS.hold @14:55:09.99 Intake @14.8psia, vacuum from 9.3 to 12.3psi @14:55:10.00 SS.reconfigure :inhaling 0.78ml/s @14:55:10.05 SS.seek 25ml @14:55:10.66 Stroke completed -- waiting for pressure to equalize ... @14:55:10.67 SS.reconfigure :exhale @14:55:10.71 SS.hold @14:55:16.43 Intake.select :close @14:55:16.56 SSV.dial :exhaust @14:55:17.32 Exhaust.select :open @14:55:17.43 SS.seek 0ml @14:55:26.43 Exhaust.select :close @14:55:26.53 SSV.dial CBV @14:55:26.55
CBV.dial SSV @14:55:27.32 Allowing 130s for 25.0ml of remaining 250.0ml @14:55:27.33 Intake.select :open @14:55:29.48 Intake @16.0psia, vacuum from 10.0 to 13.0psi @14:55:29.50 SS.reconfigure :inhaling 0.78ml/s @14:55:29.54 SS.seek 25ml @14:56:03.89 Stroke completed -- waiting for pressure to equalize ... @14:56:03.90 SS.reconfigure :exhale @14:56:03.95 SS.hold @14:56:13.62 Intake.select :close @14:56:13.76 SSV.dial :exhaust @14:56:14.51 Exhaust.select :open @14:56:14.61 SS.seek 0ml @14:56:23.46 Exhaust.select :close @14:56:23.56 SSV.dial CBV @14:56:23.58
CBV.dial SSV @14:56:24.33 Allowing 130s for 25.0ml of remaining 225.0ml @14:56:24.34 Intake.select :open @14:56:26.50 Intake @16.1psia, vacuum from 10.0 to 13.0psi @14:56:26.51 SS.reconfigure :inhaling 0.78ml/s @14:56:26.56 SS.seek 25ml @14:57:01.36 Stroke completed -- waiting for pressure to equalize ... @14:57:01.37 SS.reconfigure :exhale @14:57:01.41 SS.hold @14:57:11.09 Intake.select :close @14:57:11.23 SSV.dial :exhaust @14:57:11.98 Exhaust.select :open @14:57:12.09 SS.seek 0ml @14:57:20.92 Exhaust.select :close @14:57:21.03 SSV.dial CBV @14:57:21.05
CBV.dial SSV @14:57:21.80 Allowing 130s for 25.0ml of remaining 200.0ml @14:57:21.81 Intake.select :open @14:57:23.97 Intake @16.2psia, vacuum from 10.0 to 13.0psi @14:57:24.35 SS.reconfigure :inhaling 0.78ml/s @14:57:24.49 SS.seek 25ml @14:57:59.25 Stroke completed -- waiting for pressure to equalize ... @14:57:59.26 SS.reconfigure :exhale @14:57:59.30 SS.hold @14:58:10.37 Intake.select :close @14:58:10.51 SSV.dial :exhaust @14:58:11.25 Exhaust.select :open @14:58:11.36 SS.seek 0ml @14:58:20.49 Exhaust.select :close @14:58:20.59 SSV.dial CBV @14:58:20.61
CBV.dial SSV @14:58:21.37 Allowing 130s for 25.0ml of remaining 175.0ml @14:58:21.38 Intake.select :open @14:58:23.53 Intake @16.2psia, vacuum from 10.0 to 13.0psi @14:58:23.55 SS.reconfigure :inhaling 0.78ml/s @14:58:23.59 SS.seek 25ml @14:58:56.20 Vacuum too high -- waiting up to 40 seconds @14:58:56.21 SS.reconfigure :exhale @14:58:56.25 SS.hold @14:59:01.88 Intake @15.5psia, vacuum from 10.0 to 13.0psi @14:59:01.90 SS.reconfigure :inhaling 0.70ml/s @14:59:01.94 SS.seek 25ml @14:59:03.78 Stroke completed -- waiting for pressure to equalize ... @14:59:03.79 SS.reconfigure :exhale @14:59:03.83 SS.hold @14:59:10.25 Intake.select :close @14:59:10.39 SSV.dial :exhaust @14:59:11.13 Exhaust.select :open @14:59:11.24 SS.seek 0ml @14:59:20.09 Exhaust.select :close @14:59:20.20 SSV.dial CBV @14:59:20.22
CBV.dial SSV @14:59:20.97 Allowing 130s for 25.0ml of remaining 150.0ml @14:59:20.98 Intake.select :open @14:59:23.15 Intake @16.2psia, vacuum from 10.0 to 13.0psi @14:59:23.17 SS.reconfigure :inhaling 0.70ml/s @14:59:23.21 SS.seek 25ml @15:00:01.33 Stroke completed -- waiting for pressure to equalize ... @15:00:01.34 SS.reconfigure :exhale @15:00:01.38 SS.hold @15:00:12.40 Intake.select :close @15:00:12.55 SSV.dial :exhaust @15:00:13.30 Exhaust.select :open @15:00:13.40 SS.seek 0ml @15:00:22.47 Exhaust.select :close @15:00:22.57 SSV.dial CBV @15:00:22.59
CBV.dial SSV @15:00:23.35 Allowing 130s for 25.0ml of remaining 125.0ml @15:00:23.36 Intake.select :open @15:00:25.51 Intake @16.2psia, vacuum from 10.0 to 13.0psi @15:00:25.53 SS.reconfigure :inhaling 0.70ml/s @15:00:25.57 SS.seek 25ml @15:01:03.15 Stroke completed -- waiting for pressure to equalize ... @15:01:03.16 SS.reconfigure :exhale @15:01:03.20 SS.hold @15:01:14.42 Intake.select :close @15:01:14.56 SSV.dial :exhaust @15:01:15.30 Exhaust.select :open @15:01:15.40 SS.seek 0ml @15:01:24.23 Exhaust.select :close @15:01:24.34 SSV.dial CBV @15:01:24.37
CBV.dial SSV @15:01:25.11 Allowing 130s for 25.0ml of remaining 99.9ml @15:01:25.12 Intake.select :open @15:01:27.28 Intake @16.3psia, vacuum from 10.0 to 13.0psi @15:01:27.29 SS.reconfigure :inhaling 0.70ml/s @15:01:27.34 SS.seek 25ml @15:01:56.98 Vacuum too high -- waiting up to 40 seconds @15:01:56.99 SS.reconfigure :exhale @15:01:57.03 SS.hold @15:02:01.20 Intake @15.5psia, vacuum from 10.0 to 13.0psi @15:02:01.21 SS.reconfigure :inhaling 0.63ml/s @15:02:01.26 SS.seek 25ml @15:02:11.18 Stroke completed -- waiting for pressure to equalize ... @15:02:11.19 SS.reconfigure :exhale @15:02:11.24 SS.hold @15:02:19.78 Intake.select :close @15:02:19.92 SSV.dial :exhaust @15:02:20.67 Exhaust.select :open @15:02:20.78 SS.seek 0ml @15:02:29.43 Exhaust.select :close @15:02:29.54 SSV.dial CBV @15:02:29.56
CBV.dial SSV @15:02:30.33 Allowing 130s for 25.0ml of remaining 74.9ml @15:02:30.34 Intake.select :open @15:02:32.49 Intake @16.2psia, vacuum from 10.0 to 13.0psi @15:02:32.51 SS.reconfigure :inhaling 0.63ml/s @15:02:32.55 SS.seek 25ml @15:03:15.14 Stroke completed -- waiting for pressure to equalize ... @15:03:15.15 SS.reconfigure :exhale @15:03:15.19 SS.hold @15:03:26.26 Intake.select :close @15:03:26.40 SSV.dial :exhaust @15:03:27.14 Exhaust.select :open @15:03:27.24 SS.seek 0ml @15:03:35.98 Exhaust.select :close @15:03:36.08 SSV.dial CBV @15:03:36.11
CBV.dial SSV @15:03:36.86 Allowing 130s for 25.0ml of remaining 49.9ml @15:03:36.87 Intake.select :open @15:03:39.02 Intake @16.3psia, vacuum from 10.0 to 13.0psi @15:03:39.04 SS.reconfigure :inhaling 0.63ml/s @15:03:39.08 SS.seek 25ml @15:04:18.78 Vacuum too high -- waiting up to 40 seconds @15:04:18.79 SS.reconfigure :exhale @15:04:18.83 SS.hold @15:04:23.69 Intake @15.6psia, vacuum from 10.0 to 13.0psi @15:04:23.71 SS.reconfigure :inhaling 0.57ml/s @15:04:23.75 SS.seek 25ml @15:04:27.40 Stroke completed -- waiting for pressure to equalize ... @15:04:27.41 SS.reconfigure :exhale @15:04:27.45 SS.hold @15:04:34.93 Intake.select :close @15:04:35.07 SSV.dial :exhaust @15:04:35.81 Exhaust.select :open @15:04:35.92 SS.seek 0ml @15:04:44.95 Exhaust.select :close @15:04:45.05 SSV.dial CBV @15:04:45.07
CBV.dial SSV @15:04:45.82 Allowing 130s for 24.9ml of remaining 24.9ml @15:04:45.84 Intake.select :open @15:04:47.99 Intake @16.3psia, vacuum from 10.0 to 13.0psi @15:04:48.01 SS.reconfigure :inhaling 0.57ml/s @15:04:48.05 SS.seek 24.94ml @15:05:33.93 Stroke completed -- waiting for pressure to equalize ... @15:05:33.94 SS.reconfigure :exhale @15:05:33.98 SS.hold @15:05:45.13 Intake.select :close @15:05:45.27 SSV.dial :exhaust @15:05:46.01 Exhaust.select :open @15:05:46.12 SS.seek 0ml @15:05:54.88 Exhaust.select :close @15:05:54.99 Sampled 1000.0ml @15:05:55.00 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @15:05:55.01 Intake.select :close @15:05:55.06 CSV.dial :waste @15:05:55.11 CS.seek 0ml @15:05:55.21 CSV.dial :air @15:05:55.24 SSV.dial CBV,avoiding: :exhaust @15:05:55.26
CBV.dial SSV @15:05:56.01 CS.seek 10ml @15:05:57.14
CTV.dial :air @15:05:57.93 delay 15 seconds @15:06:12.95 SSV.dial :spit,avoiding: :exhaust @15:06:14.28 CTV.dial CSV,avoiding: :intake @15:06:14.31
SS.seek 0ml @15:06:14.43 SSV.dial CBV,avoiding: :exhaust @15:06:20.59 CBV.dial between SSV and waste2 @15:06:20.98 CTV.dial CSV,avoiding: :air @15:06:21.00
CSV.dial CTV @15:06:21.56 CS.reconfigure :pressurize @15:06:21.60 CS.seek 0ml @15:06:40.16 BubblePoint: Intake @39.4psia, Outlet @15.0psia @15:06:40.17 CBV.dial SSV @15:06:40.51 SS.reconfigure :default @15:06:40.55 SS.seek 10ml @15:06:46.18 delay 15 seconds @15:07:01.30 Evacuated: Intake @17.5psia, Outlet @10.2psia @15:07:01.31 SSV.dial :spit,avoiding: :exhaust @15:07:02.64 SS.reconfigure :exhale @15:07:02.68 SS.seek 0ml @15:07:08.11 SS.reconfigure :fast @15:07:08.15 SS.seek 5ml @15:07:11.00 SSV.dial CBV,avoiding: :exhaust @15:07:11.02
CBV.dial SSV @15:07:12.34 CTV.dial :air,avoiding: :intake @15:07:13.69 CSV.dial CTV @15:07:13.72
SS.seek 10ml @15:07:14.73 CSV.dial :air @15:07:15.16 CS.reconfigure :fast @15:07:15.21 CS.seek 0ml @15:07:16.21
SSV.dial :spit,avoiding: :exhaust @15:07:17.53 SS.reconfigure :exhale @15:07:17.57 SS.seek 0ml @15:07:22.67 SS.reconfigure :fast @15:07:22.71 SS.seek 5ml @15:07:22.81 CS.stop @15:07:25.58 SSV.dial CBV,avoiding: :exhaust @15:07:25.60
CBV.dial SSV @15:07:27.92 SSV.dial :spit,avoiding: :exhaust @15:07:29.70 SS.reconfigure :default @15:07:29.74 SS.seek 0ml @15:07:33.03 SS.stop @15:07:33.07 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) Intake.select :close @15:07:33.14 CSV.dial :waste @15:07:33.26 SSV.dial CBV,avoiding: :exhaust @15:07:33.27
CBV.dial SSV @15:07:33.92 CS.seek 0ml @15:07:33.98 CSV.dial :air @15:07:34.57
CTV.dial :air @15:07:34.59 delay 15 seconds @15:07:34.71 CS.seek 10ml @15:07:49.60
SSV.dial :spit,avoiding: :exhaust @15:07:50.92 CTV.dial CSV,avoiding: :intake @15:07:50.95
SS.reconfigure :exhale @15:07:51.05 SS.seek 0ml @15:07:51.42 SSV.dial CBV,avoiding: :exhaust @15:07:59.09 CBV.dial between SSV and waste2 @15:07:59.49 CTV.dial CSV,avoiding: :air @15:07:59.51
CSV.dial CTV @15:07:59.95 CS.reconfigure :pressurize @15:07:59.99 CS.seek 0ml @15:08:20.91 BubblePoint: Intake @38.4psia, Outlet @14.1psia @15:08:20.92 CBV.dial SSV @15:08:21.24 SS.reconfigure :default @15:08:21.28 SS.seek 10ml @15:08:26.91 delay 15 seconds @15:08:42.03 Evacuated: Intake @19.0psia, Outlet @11.5psia @15:08:42.04 SSV.dial :spit,avoiding: :exhaust @15:08:43.37 SS.reconfigure :exhale @15:08:43.41 SS.seek 0ml @15:08:49.05 SS.reconfigure :fast @15:08:49.09 SS.seek 5ml @15:08:52.08 SSV.dial CBV,avoiding: :exhaust @15:08:52.09
CBV.dial SSV @15:08:53.42 CTV.dial :air,avoiding: :intake @15:08:54.75 CSV.dial CTV @15:08:54.79
SS.seek 10ml @15:08:55.78 CSV.dial :air @15:08:56.24 CS.reconfigure :fast @15:08:56.28 CS.seek 0ml @15:08:57.45
SSV.dial :spit,avoiding: :exhaust @15:08:58.76 SS.reconfigure :exhale @15:08:58.81 SS.seek 0ml @15:09:01.81 CS.stop @15:09:04.08
SS.reconfigure :fast @15:09:04.12 SS.seek 5ml @15:09:06.97 SSV.dial CBV,avoiding: :exhaust @15:09:06.99
CBV.dial SSV @15:09:09.31 SSV.dial :spit,avoiding: :exhaust @15:09:10.62 SS.reconfigure :default @15:09:10.66 SS.seek 0ml @15:09:13.86 SS.stop @15:09:13.90 Lysing: Adding 2.0ml AqMeOH @15:09:13.91 CBV.dial between CSV and waste1,avoiding: :DBV @15:09:15.39 CTV.dial CSV @15:09:16.72 CSV.dial CSR @15:09:17.51 Pulling 1ml of CSR.AqMeOH @15:09:17.52 CSR.select :AqMeOH @15:09:17.62 CS.reconfigure :default @15:09:17.67 CS.seek 1ml @15:09:21.22 delay 5 seconds @15:09:26.24 CSR.select :air @15:09:26.39 CS.seek 6ml @15:09:39.94 delay 2 seconds @15:09:41.95 CSV.dial :waste2 @15:09:42.40 CS.reconfigure :fast @15:09:42.44 CS.seek 0ml @15:09:56.81 CSV.dial CSR @15:09:57.32 Pulling 2ml of CSR.AqMeOH @15:09:57.33 CSR.select :AqMeOH @15:09:57.44 CS.reconfigure :default @15:09:57.48 CS.seek 2ml @15:10:03.47 delay 5 seconds @15:10:08.49 CSR.select :air @15:10:08.64 CS.seek 5ml @15:10:17.12 delay 2 seconds @15:10:19.14 CSV.dial CTV @15:10:19.59 CS.seek 1ml @15:10:30.95 Before lysing Collection Puck Heater at 20.7C/39.6psia @15:10:30.97 Heating Puck.from 4, :sh1 in Collection Clamp to 70C for 10:00 @15:10:30.99 CH.seek 70C,8:00 @15:12:34.02 CH.hold 10:00,70C @15:12:34.19 Begun lysing Collection Puck Heater at 68.2C/46.6psia @15:22:34.21 Ended lysing Collection Puck Heater at 70C/45.1psia @15:22:34.22 CS.seek 0ml @15:22:37.99 CTV.dial between spare and air,avoiding: :intake @15:22:39.02 CSV.dial :air @15:22:39.46 CS.seek 5ml @15:22:53.23 CSV.dial CTV @15:22:53.68 delay 2 seconds @15:22:55.69 CSV.dial :air @15:22:56.14 delay 2 seconds @15:22:58.16 CSV.dial CTV @15:22:58.61 delay 2 seconds @15:23:00.63 CSV.dial :air @15:23:01.08 delay 2 seconds @15:23:03.10 CS.seek 0ml @15:23:16.76 Puck @73.3psia after final lysis pressurization @15:23:16.77 Recovering Lysate into Collection syringe @15:23:16.78 CSV.dial CBV @15:23:17.77 CBV.dial CSV @15:23:18.13 CS.seek 5ml @15:23:31.89 delay 5 seconds @15:23:36.91 CTV.dial :air @15:23:37.44 CS.seek 7ml @15:23:43.58 delay 5 seconds @15:23:48.60 CSV.dial :air2 @15:23:49.04 CS.seek 10ml @15:23:57.61 CSV.dial between air and CTV,avoiding: CBV @15:23:58.76 Lysing Complete @15:23:58.82 Storing sh1 puck in Collection Clamp to tube 3 @15:23:58.98 SC.reconfigure :tight @15:23:59.03 SC.seek 3 @15:24:02.61 SC.configure :limp @15:24:02.80 Elbow.reconfigure :tight @15:24:02.85 Elbow.seek 3 @15:24:04.63 Forearm.reconfigure :tight @15:24:04.67 Forearm.seek 3 + 450 counts @15:24:05.71 SE.reconfigure :pick @15:24:05.76 SE.seek :beamBreak @15:24:08.09 SE.configure :default @15:24:08.24 Elbow.reconfigure :limp @15:24:08.28 Forearm.reconfigure :loose @15:24:08.32 Forearm.seek 3 @15:24:08.53 Elbow.hold @15:24:08.59 Elbow.reconfigure :tight @15:24:08.68 SE.seek 13.12 Pucks below Top Plate @15:24:09.27 Forearm.force -20 @15:24:09.31 Forearm.seek 3 + 318 counts @15:24:10.05 Forearm.reconfigure :tight @15:24:10.11 Forearm.seek 3 + 450 counts @15:24:10.30 SE.seek 14.12 Pucks below Top Plate @15:24:10.84 Forearm.reconfigure :loose @15:24:10.88 Forearm.seek :retract @15:24:11.80 Elbow.seek CC @15:24:13.47 Elbow.reconfigure :loose @15:24:13.56 Forearm.seek CC @15:24:14.48 Hand.close @15:24:15.05 CC.seek :open @15:24:19.76 Forearm.seek :retract @15:24:20.70 Elbow.reconfigure :tight @15:24:20.75 Elbow.seek 3 @15:24:22.59 Elbow.reconfigure :limp @15:24:22.64 Forearm.seek 3 @15:24:23.67 Elbow.hold @15:24:23.73 Elbow.reconfigure :tight @15:24:23.78 Hand.open @15:24:24.53 Forearm.force -20 @15:24:24.57 Forearm.seek 3 + 307 counts @15:24:24.86 Forearm.reconfigure :tight @15:24:24.91 Forearm.seek 3 + 450 counts @15:24:25.16 SE.seek :down @15:24:26.28
Forearm.reconfigure :loose @15:24:26.32 Forearm.seek :retract @15:24:26.91 SE.stop @15:24:27.25
Tranferring DA @15:24:27.26 Beginning samplePassC @15:24:27.28 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @15:24:27.46 Elbow.seek FlushPuck::Garage @15:24:29.30 Elbow.reconfigure :loose @15:24:29.40 Forearm.seek :garage @15:24:30.37 Hand.close @15:24:31.03 Forearm.seek :retract @15:24:32.08 Elbow.reconfigure :tight @15:24:32.12 Elbow.seek CC @15:24:35.13 Elbow.reconfigure :loose @15:24:35.22 Forearm.reconfigure :tight @15:24:35.26 Forearm.seek CC @15:24:36.51 CC.seek :closed @15:24:41.01 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.33 @15:24:41.08 Hand.open @15:24:41.73 Forearm.reconfigure :loose @15:24:41.77 Forearm.seek :retract @15:24:42.71 PSV.dial PSR @15:24:44.08 Pulling 1.5ml of PSR.flush @15:24:44.09 PSR.select :flush @15:24:44.20 PS.seek 1.5ml @15:25:00.34 delay 5 seconds @15:25:05.36 PSV.dial :waste1 @15:25:05.81 PS.seek 0ml @15:25:21.99 PSV.dial PSR @15:25:22.44 PSR.select :air @15:25:22.55 PS.seek 2.5ml @15:25:48.93 PRV.dial CBV,avoiding: :ab2 @15:25:48.95
CBV.dial PRV @15:25:50.63 PSV.dial :mixloop @15:25:51.66 CTV.dial :air,avoiding: :intake @15:25:51.67 PTV.dial :PRVmixing @15:25:53.00 PS.seek 0ml @15:26:19.36 PSV.dial :air @15:26:19.82 PS.seek 2.5ml @15:26:46.24 PSV.dial :mixloop @15:26:46.70 PS.seek 0ml @15:27:13.05 PSV.dial :air @15:27:13.99 PS.seek 2.5ml @15:27:40.36 PSV.dial :mixloop @15:27:40.82 PS.seek 0ml @15:28:07.18 Garaging FlushPuck in Collection Clamp @15:28:07.25 Elbow.reconfigure :tight @15:28:07.29 Elbow.seek CC @15:28:07.39 Elbow.reconfigure :loose @15:28:07.49 Forearm.seek CC @15:28:08.51 Hand.close @15:28:09.17 CC.seek :open @15:28:13.81 Forearm.seek :retract @15:28:14.79 Elbow.reconfigure :tight @15:28:14.84 Elbow.seek :garage @15:28:17.74 Elbow.reconfigure :loose @15:28:17.83 Forearm.reconfigure :tight @15:28:17.87 Forearm.seek :garage @15:28:18.86 Hand.open @15:28:19.48 Forearm.reconfigure :loose @15:28:19.52 Forearm.seek :retract @15:28:20.48 PSV.dial :mixloop @15:28:20.49 PTV.dial :PRVmixing @15:28:20.50 PRV.dial CBV @15:28:20.53
CBV.dial PRV @15:28:20.54 CTV.dial :air,avoiding: :intake @15:28:20.56 Loading lyfil puck from tube 4 into Collection Clamp @15:28:20.78 SC.reconfigure :tight @15:28:20.82 SC.seek 4 @15:28:24.40 SC.configure :limp @15:28:24.59 Elbow.reconfigure :tight @15:28:24.64 Elbow.seek 4 @15:28:26.59 Forearm.reconfigure :tight @15:28:26.64 Forearm.seek 4 + 450 counts @15:28:27.72 SE.reconfigure :pick @15:28:27.77 SE.seek :beamBreak @15:28:29.79 SE.configure :default @15:28:29.93 Elbow.seek 4 @15:28:30.11 Forearm.seek 4 + 300 counts @15:28:30.29 Forearm.reconfigure :loose @15:28:30.33 Hand.close @15:28:30.93 SE.seek :down @15:28:31.12
Forearm.seek :retract @15:28:32.06 Elbow.seek CC @15:28:32.60 SE.stop @15:28:33.71
Elbow.reconfigure :loose @15:28:33.80 Forearm.reconfigure :tight @15:28:33.84 Forearm.seek CC @15:28:35.10 CC.seek :closed @15:28:39.60 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.04 @15:28:39.61 Hand.open @15:28:40.23 Forearm.reconfigure :loose @15:28:40.27 Forearm.seek :retract @15:28:41.23 CTV.dial CSV,avoiding: :intake @15:28:42.59 CSV.dial CTV @15:28:43.02 PS.seek 2.5ml @15:29:09.39 CS.seek 4.5ml @15:29:24.39 delay 8 seconds @15:29:32.41 PSV.dial :air @15:29:32.88 delay 2 seconds @15:29:34.89 PSV.dial :waste2 @15:29:35.63 PS.seek 0ml @15:30:01.95 CBV.dial between PRV and waste1 @15:30:02.28 CSV.dial :air @15:30:02.72 CS.seek 10ml @15:30:18.29 CSV.dial CTV @15:30:18.75 CS.seek 0ml @15:30:45.13 CBV.dial :waste1 @15:30:45.43 delay 5 seconds @15:30:50.45 CTV.dial :air,avoiding: :intake @15:30:51.85 Storing lyfil puck in Collection Clamp to tube 3 @15:30:52.02 SC.reconfigure :tight @15:30:52.06 SC.seek 3 @15:30:55.66 SC.configure :limp @15:30:55.86 Elbow.reconfigure :tight @15:30:55.90 Elbow.seek 3 @15:30:57.68 Forearm.reconfigure :tight @15:30:57.73 Forearm.seek 3 + 450 counts @15:30:58.77 SE.reconfigure :pick @15:30:58.82 SE.seek :beamBreak @15:31:01.07 SE.configure :default @15:31:01.22 Elbow.reconfigure :limp @15:31:01.26 Forearm.reconfigure :loose @15:31:01.30 Forearm.seek 3 @15:31:01.51 Elbow.hold @15:31:01.57 Elbow.reconfigure :tight @15:31:01.66 SE.seek 14.14 Pucks below Top Plate @15:31:02.18 Forearm.force -20 @15:31:02.21 Forearm.seek 3 + 314 counts @15:31:02.48 Forearm.reconfigure :tight @15:31:02.53 Forearm.seek 3 + 450 counts @15:31:02.73 SE.seek 15.14 Pucks below Top Plate @15:31:03.23 Forearm.reconfigure :loose @15:31:03.27 Forearm.seek :retract @15:31:04.20 Elbow.seek CC @15:31:05.88 Elbow.reconfigure :loose @15:31:05.96 Forearm.seek CC @15:31:06.96 Hand.close @15:31:07.54 CC.seek :open @15:31:12.29 Forearm.seek :retract @15:31:13.29 Elbow.reconfigure :tight @15:31:13.33 Elbow.seek 3 @15:31:15.08 Elbow.reconfigure :limp @15:31:15.13 Forearm.seek 3 @15:31:16.10 Elbow.hold @15:31:16.16 Elbow.reconfigure :tight @15:31:16.20 Hand.open @15:31:16.90 Forearm.force -20 @15:31:16.93 Forearm.seek 3 + 315 counts @15:31:17.22 Forearm.reconfigure :tight @15:31:17.27 Forearm.seek 3 + 450 counts @15:31:17.53 SE.seek :down @15:31:18.65
Forearm.reconfigure :loose @15:31:18.70 Forearm.seek :retract @15:31:19.20 SE.stop @15:31:19.63
PSV.dial :air @15:31:20.38 PS.seek 2.5ml @15:31:46.76 delay 1 second @15:31:47.78 PSV.dial :mixloop @15:31:48.20 delay 1 second @15:31:49.22 PSV.dial :air @15:31:49.67 delay 1 second @15:31:50.69 PSV.dial :mixloop @15:31:51.15 delay 1 second @15:31:52.17 PSV.dial :air @15:31:52.64 delay 1 second @15:31:53.66 PS.seek 0ml @15:32:20.17 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @15:32:20.34 Elbow.seek FlushPuck::Garage @15:32:22.08 Elbow.reconfigure :loose @15:32:22.16 Forearm.seek :garage @15:32:23.16 Hand.close @15:32:23.90 Forearm.seek :retract @15:32:25.33 Elbow.reconfigure :tight @15:32:25.37 Elbow.seek PC @15:32:26.80 Elbow.reconfigure :loose @15:32:26.89 Forearm.reconfigure :tight @15:32:26.93 Forearm.seek PC @15:32:28.22 PC.seek :closed @15:32:32.87 Processing Clamp onSpring/offSpring for 16/26 samples, on/off torque 1.55 @15:32:32.94 Hand.open @15:32:33.60 Forearm.reconfigure :loose @15:32:33.64 Forearm.seek :retract @15:32:34.58 PRV.dial :air @15:32:35.03 PSV.dial :mixloop @15:32:35.56 PS.seek 0.3ml @15:32:39.39 PSV.dial PSR @15:32:40.47 Pulling 0.4ml of PSR.air @15:32:40.48 PSR.select :air @15:32:40.51 PS.seek 0.7ml @15:32:45.36 delay 3 seconds @15:32:48.37 PSR.select :air @15:32:48.39 PS.seek 2.5ml @15:33:07.79 delay 3 seconds @15:33:10.81 PSV.dial :mixloop @15:33:11.84 PTV.dial :mixing @15:33:12.59 PBV.dial :waste2 @15:33:13.52 PS.seek 0ml @15:33:39.92 Garaging FlushPuck in Processing Clamp @15:33:39.99 Elbow.reconfigure :tight @15:33:40.03 Elbow.seek PC @15:33:40.14 Elbow.reconfigure :loose @15:33:40.23 Forearm.seek PC @15:33:41.21 Hand.close @15:33:41.79 PC.seek :open @15:33:46.50 Forearm.seek :retract @15:33:47.43 Elbow.reconfigure :tight @15:33:47.47 Elbow.seek :garage @15:33:48.77 Elbow.reconfigure :loose @15:33:48.86 Forearm.reconfigure :tight @15:33:48.90 Forearm.seek :garage @15:33:49.93 Hand.open @15:33:50.60 Forearm.reconfigure :loose @15:33:50.64 Forearm.seek :retract @15:33:51.60 Loading da puck from tube 4 into Processing Clamp @15:33:51.82 SC.reconfigure :tight @15:33:51.87 SC.seek 4 @15:33:55.47 SC.configure :limp @15:33:55.66 Elbow.reconfigure :tight @15:33:55.70 Elbow.seek 4 @15:33:57.54 Forearm.reconfigure :tight @15:33:57.58 Forearm.seek 4 + 450 counts @15:33:58.63 SE.reconfigure :pick @15:33:58.68 SE.seek :beamBreak @15:34:00.77 SE.configure :default @15:34:00.91 Elbow.seek 4 @15:34:01.09 Forearm.seek 4 + 300 counts @15:34:01.33 Forearm.reconfigure :loose @15:34:01.38 Hand.close @15:34:02.05 SE.seek :down @15:34:02.24
Forearm.seek :retract @15:34:03.15 Elbow.seek PC @15:34:03.74 SE.stop @15:34:05.66
Elbow.reconfigure :loose @15:34:05.75 Forearm.reconfigure :tight @15:34:05.79 Forearm.seek PC @15:34:06.98 PC.seek :closed @15:34:11.60 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.57 @15:34:11.61 Hand.open @15:34:12.22 Forearm.reconfigure :loose @15:34:12.26 Forearm.seek :retract @15:34:13.26 Heating Puck.from 4, :da in Processing Clamp to 28C for 2:00:00 @15:34:13.27 PH.seek 28C,5:00 @15:34:43.77 PH.hold 2:00:00,28C @15:34:43.82 delay 1:30 @15:36:13.86 PSR.select :air @15:36:13.88 PSV.dial PSR @15:36:14.97 Pulling 0.5ml of PSR.wash @15:36:14.98 PSR.select :wash @15:36:15.10 PS.seek 0.5ml @15:36:21.01 delay 5 seconds @15:36:26.03 PSR.select :air @15:36:26.14 PS.seek 2ml @15:36:42.51 PTV.dial :puck @15:36:43.24 PSV.dial PTV @15:36:43.99 PBV.dial :waste1 @15:36:45.38 PS.seek 1.5ml @15:36:51.35 delay 3 seconds @15:36:54.37 PBV.dial between PSV and waste1 @15:36:54.72 PS.seek 0ml @15:37:10.82 delay 5:00 @15:42:10.84 recoverPSRreagent08 @15:42:10.85 PTV.dial :puck @15:42:10.87 PBV.dial :waste1 @15:42:11.22 delay 30 seconds @15:42:41.24 PBV.dial between PSV and waste1 @15:42:41.60 PSR.select :air @15:42:41.61 PSV.dial PSR @15:42:42.37 PS.reconfigure :fast @15:42:42.41 PS.seek 2.5ml @15:43:05.90 PSV.dial PTV @15:43:06.64 delay 5 seconds @15:43:11.66 PSV.dial PSR @15:43:12.41 delay 5 seconds @15:43:17.43 PSV.dial PTV @15:43:18.17 delay 5 seconds @15:43:23.19 PSV.dial :air @15:43:23.94 PS.seek 0ml @15:43:46.97 PTV.dial :PRVmixing,via: PRV @15:43:48.26 PSV.dial :mixloop @15:43:48.73 PRV.dial :ab1 @15:43:49.49 PS.reconfigure :slow @15:43:49.53 PS.seek 0.15ml @15:43:53.31 delay 2 seconds @15:43:55.33 CBV.dial PRV @15:43:55.35
PRV.dial CBV @15:43:55.91 PS.seek 0.35ml @15:44:00.56 delay 2 seconds @15:44:02.58 CBV.dial between PRV and PSV @15:44:02.90 PRV.dial :air @15:44:03.37 PS.seek 0.9ml @15:44:15.12 PRV.dial :waste2 @15:44:15.85 PS.seek 0.5ml @15:44:24.59 addPMLReagent08 @15:44:24.60 PSV.dial :air @15:44:25.07 PS.reconfigure :default @15:44:25.11 PS.seek 2ml @15:44:41.21 PSV.dial :PML @15:44:41.67 PTV.dial :mixing @15:44:42.42 PBV.dial :waste1 @15:44:42.84 PS.seek 1.85ml @15:44:45.28 PBV.dial between PSV and waste1 @15:44:45.66 PS.seek 0ml @15:45:05.56 delay 2 seconds @15:45:07.58 Beginning samplePassC @15:45:07.59 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @15:45:07.76 Elbow.reconfigure :tight @15:45:07.80 Elbow.seek FlushPuck::Garage @15:45:09.13 Elbow.reconfigure :loose @15:45:09.22 Forearm.seek :garage @15:45:10.21 Hand.close @15:45:11.38 Forearm.seek :retract @15:45:12.36 Elbow.reconfigure :tight @15:45:12.40 Elbow.seek CC @15:45:15.27 Elbow.reconfigure :loose @15:45:15.36 Forearm.reconfigure :tight @15:45:15.41 Forearm.seek CC @15:45:16.62 CC.seek :closed @15:45:21.15 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.31 @15:45:21.21 Hand.open @15:45:21.84 Forearm.reconfigure :loose @15:45:21.88 Forearm.seek :retract @15:45:22.82 PSV.dial PSR @15:45:24.02 Pulling 1.5ml of PSR.flush @15:45:24.03 PSR.select :flush @15:45:24.15 PS.seek 1.5ml @15:45:40.28 delay 5 seconds @15:45:45.30 PSV.dial :waste1 @15:45:45.77 PS.seek 0ml @15:46:01.93 PSV.dial PSR @15:46:02.51 PSR.select :air @15:46:02.62 PS.seek 2.5ml @15:46:29.00 PRV.dial CBV,avoiding: :ab2 @15:46:29.02
CBV.dial PRV @15:46:30.62 PSV.dial :mixloop @15:46:31.67 CTV.dial :air,avoiding: :intake @15:46:31.68 PTV.dial :PRVmixing @15:46:32.42 PS.seek 0ml @15:46:58.84 PSV.dial :air @15:46:59.41 PS.seek 2.5ml @15:47:25.84 PSV.dial :mixloop @15:47:26.28 PS.seek 0ml @15:47:52.68 PSV.dial :air @15:47:53.14 PS.seek 2.5ml @15:48:19.52 PSV.dial :mixloop @15:48:19.98 PS.seek 0ml @15:48:46.34 Garaging FlushPuck in Collection Clamp @15:48:46.41 Elbow.reconfigure :tight @15:48:46.45 Elbow.seek CC @15:48:46.99 Elbow.reconfigure :loose @15:48:47.07 Forearm.seek CC @15:48:48.07 Hand.close @15:48:48.65 CC.seek :open @15:48:53.29 Forearm.seek :retract @15:48:54.26 Elbow.reconfigure :tight @15:48:54.30 Elbow.seek :garage @15:48:57.20 Elbow.reconfigure :loose @15:48:57.29 Forearm.reconfigure :tight @15:48:57.34 Forearm.seek :garage @15:48:58.34 Hand.open @15:48:58.96 Forearm.reconfigure :loose @15:48:59.00 Forearm.seek :retract @15:48:59.96 delayUntil "15:53:07.57" @15:48:59.99 Whole Cell Archive sampling 1000ml @15:49:00.00 Loading wcr puck from tube 4 into Collection Clamp @15:49:00.45 Elbow.reconfigure :tight @15:49:00.50 Elbow.seek 4 @15:49:02.41 Forearm.reconfigure :tight @15:49:02.45 Forearm.seek 4 + 450 counts @15:49:03.50 SE.reconfigure :pick @15:49:03.55 SE.seek :beamBreak @15:49:05.70 SE.configure :default @15:49:05.84 Elbow.seek 4 @15:49:06.49 Forearm.seek 4 + 300 counts @15:49:06.70 Forearm.reconfigure :loose @15:49:06.74 Hand.close @15:49:07.42 SE.seek :down @15:49:07.63 Forearm.seek :retract @15:49:08.56 Elbow.seek CC @15:49:09.19 SE.stop @15:49:10.12 Elbow.reconfigure :loose @15:49:10.22 Forearm.reconfigure :tight @15:49:10.27 Forearm.seek CC @15:49:11.59 CC.seek :closed @15:49:16.13 Collection Clamp onSpring/offSpring for 14/27 samples, on/off torque 2.13 @15:49:16.14 Hand.open @15:49:16.83 Forearm.reconfigure :loose @15:49:16.88 Forearm.seek :retract @15:49:17.87 collectSample 1000ml w/bubble point of 35psi @15:49:17.88 Priming 7ml @15:49:17.89 Intake.select :close @15:49:17.90 Exhaust.select :close @15:49:17.91 SS.hold @15:49:18.11 Calibrating pressure sensors @15:49:18.12 CTV.dial :air @15:49:18.14 SSV.dial :spit,avoiding: :exhaust @15:49:18.15 SS.reconfigure :fast @15:49:18.20 SS.seek 5ml @15:49:20.83 SSV.dial CBV @15:49:20.86 CBV.dial SSV @15:49:22.19 delay 3.8 seconds @15:49:26.13 SSV.dial :spit @15:49:27.46 SS.seek 0ml @15:49:30.21 SSV.dial CBV @15:49:32.30 CTV.dial :intake @15:49:33.06 SSV.dial CBV @15:49:33.08 CBV.dial SSV @15:49:33.10 Allowing 40s for 7.0ml of remaining 7.0ml @15:49:33.11 Intake.select :open @15:49:35.26 Intake @15.1psia, vacuum from 9.6 to 12.6psi @15:49:35.28 SS.reconfigure :inhaling 2.20ml/s @15:49:35.32 SS.seek 7ml @15:49:41.94 Stroke completed -- waiting for pressure to equalize ... @15:49:41.95 SS.reconfigure :exhale @15:49:41.99 SS.hold @15:49:56.28 Intake.select :close @15:49:56.42 SSV.dial :exhaust @15:49:57.17 Exhaust.select :open @15:49:57.29 SS.seek 0ml @15:50:01.44 Exhaust.select :close @15:50:01.54 Sampling 1000.0ml @15:50:01.55 Intake.select :close @15:50:01.56 Exhaust.select :close @15:50:01.58 SS.reconfigure :default @15:50:01.62 SS.hold @15:50:01.81 CTV.dial :intake @15:50:01.83 SSV.dial CBV @15:50:01.86 CBV.dial SSV @15:50:02.60 Allowing 130s for 25.0ml of remaining 1000.0ml @15:50:02.61 Intake.select :open @15:50:04.76 Intake @15.8psia, vacuum from 10.0 to 13.0psi @15:50:04.78 SS.reconfigure :inhaling 2.20ml/s @15:50:05.30 SS.seek 25ml @15:50:11.54 Vacuum too high -- waiting up to 40 seconds @15:50:11.55 SS.reconfigure :exhale @15:50:11.60 SS.hold @15:50:16.06 Intake @15.8psia, vacuum from 10.0 to 13.0psi @15:50:16.08 SS.reconfigure :inhaling 1.98ml/s @15:50:16.12 SS.seek 25ml @15:50:21.42 Vacuum too high -- waiting up to 40 seconds @15:50:21.43 SS.reconfigure :exhale @15:50:21.47 SS.hold @15:50:27.34 Intake @17.0psia, vacuum from 10.0 to 13.0psi @15:50:27.36 SS.reconfigure :inhaling 1.79ml/s @15:50:27.41 SS.seek 25ml @15:50:31.32 Vacuum too high -- waiting up to 40 seconds @15:50:31.33 SS.reconfigure :exhale @15:50:31.38 SS.hold @15:50:34.58 Intake @16.7psia, vacuum from 10.0 to 13.0psi @15:50:34.60 SS.reconfigure :inhaling 1.61ml/s @15:50:34.64 SS.seek 25ml @15:50:37.69 Stroke completed -- waiting for pressure to equalize ... @15:50:37.70 SS.reconfigure :exhale @15:50:37.74 SS.hold @15:50:41.83 Intake.select :close @15:50:41.97 SSV.dial :exhaust @15:50:42.73 Exhaust.select :open @15:50:42.84 SS.seek 0ml @15:50:51.72 Exhaust.select :close @15:50:51.83 SSV.dial CBV @15:50:51.87 CBV.dial SSV @15:50:52.60 Allowing 130s for 25.0ml of remaining 975.0ml @15:50:52.61 Intake.select :open @15:50:54.76 Intake @16.6psia, vacuum from 10.0 to 13.0psi @15:50:54.78 SS.reconfigure :inhaling 1.61ml/s @15:50:54.83 SS.seek 25ml @15:51:02.16 Vacuum too high -- waiting up to 40 seconds @15:51:02.18 SS.reconfigure :exhale @15:51:02.22 SS.hold @15:51:05.40 Intake @16.8psia, vacuum from 10.0 to 13.0psi @15:51:05.42 SS.reconfigure :inhaling 1.45ml/s @15:51:05.46 SS.seek 25ml @15:51:11.01 Vacuum too high -- waiting up to 40 seconds @15:51:11.02 SS.reconfigure :exhale @15:51:11.06 SS.hold @15:51:14.72 Intake @16.9psia, vacuum from 10.0 to 13.0psi @15:51:14.74 SS.reconfigure :inhaling 1.30ml/s @15:51:14.78 SS.seek 25ml @15:51:20.98 Vacuum too high -- waiting up to 40 seconds @15:51:20.99 SS.reconfigure :exhale @15:51:21.03 SS.hold @15:51:24.36 Intake @17.0psia, vacuum from 10.0 to 13.0psi @15:51:24.38 SS.reconfigure :inhaling 1.17ml/s @15:51:24.43 SS.seek 25ml @15:51:27.67 Stroke completed -- waiting for pressure to equalize ... @15:51:27.68 SS.reconfigure :exhale @15:51:27.72 SS.hold @15:51:32.74 Intake.select :close @15:51:32.88 SSV.dial :exhaust @15:51:33.62 Exhaust.select :open @15:51:33.74 SS.seek 0ml @15:51:42.58 Exhaust.select :close @15:51:42.69 SSV.dial CBV @15:51:42.72 CBV.dial SSV @15:51:43.47 Allowing 130s for 25.0ml of remaining 950.0ml @15:51:43.48 Intake.select :open @15:51:45.64 Intake @16.7psia, vacuum from 10.0 to 13.0psi @15:51:45.66 SS.reconfigure :inhaling 1.17ml/s @15:51:45.70 SS.seek 25ml @15:51:57.07 Vacuum too high -- waiting up to 40 seconds @15:51:57.08 SS.reconfigure :exhale @15:51:57.12 SS.hold @15:51:59.64 Intake @16.0psia, vacuum from 10.0 to 13.0psi @15:51:59.66 SS.reconfigure :inhaling 1.06ml/s @15:51:59.70 SS.seek 25ml @15:52:14.48 Stroke completed -- waiting for pressure to equalize ... @15:52:14.49 SS.reconfigure :exhale @15:52:14.54 SS.hold @15:52:23.06 Intake.select :close @15:52:23.20 SSV.dial :exhaust @15:52:23.97 Exhaust.select :open @15:52:24.08 SS.seek 0ml @15:52:32.84 Exhaust.select :close @15:52:32.95 SSV.dial CBV @15:52:32.97 CBV.dial SSV @15:52:33.75 Allowing 130s for 25.0ml of remaining 925.0ml @15:52:33.76 Intake.select :open @15:52:35.92 Intake @16.6psia, vacuum from 10.0 to 13.0psi @15:52:35.94 SS.reconfigure :inhaling 1.06ml/s @15:52:35.98 SS.seek 25ml @15:52:49.88 Vacuum too high -- waiting up to 40 seconds @15:52:49.89 SS.reconfigure :exhale @15:52:49.94 SS.hold @15:52:53.49 Intake @16.6psia, vacuum from 10.0 to 13.0psi @15:52:53.51 SS.reconfigure :inhaling 0.95ml/s @15:52:53.56 SS.seek 25ml @15:53:07.57 recoverPRVreagent08 @15:53:07.59 PTV.dial :mixing @15:53:07.70 Stroke completed -- waiting for pressure to equalize ... @15:53:07.71 SS.reconfigure :exhale @15:53:07.75 SS.hold @15:53:08.33 PBV.dial :waste1 @15:53:08.69 delay 30 seconds @15:53:15.64 Intake.select :close @15:53:15.78 SSV.dial :exhaust @15:53:16.54 Exhaust.select :open @15:53:16.66 SS.seek 0ml @15:53:25.45 Exhaust.select :close @15:53:25.56 SSV.dial CBV @15:53:25.58 CBV.dial SSV @15:53:26.36 Allowing 130s for 25.0ml of remaining 900.0ml @15:53:26.37 Intake.select :open @15:53:28.54 Intake @16.5psia, vacuum from 10.0 to 13.0psi @15:53:28.55 SS.reconfigure :inhaling 0.95ml/s @15:53:28.60 SS.seek 25ml @15:53:38.71 PBV.dial between PSV and waste1 @15:53:39.08 PSV.dial :air @15:53:39.56 PS.reconfigure :fast @15:53:39.60 PS.seek 2.5ml @15:53:48.54 Vacuum too high -- waiting up to 40 seconds @15:53:48.55 SS.reconfigure :exhale @15:53:48.59 SS.hold @15:53:52.78 Intake @16.4psia, vacuum from 10.0 to 13.0psi @15:53:52.80 SS.reconfigure :inhaling 0.87ml/s @15:53:52.84 SS.seek 25ml @15:54:03.14 Stroke completed -- waiting for pressure to equalize ... @15:54:03.16 SS.reconfigure :exhale @15:54:03.20 SS.hold @15:54:03.52 PSV.dial :mixloop @15:54:04.00 delay 2 seconds @15:54:06.08 PSV.dial :air @15:54:06.53 delay 2 seconds @15:54:08.55 PSV.dial :mixloop @15:54:09.02 delay 2 seconds @15:54:09.66 Intake.select :close @15:54:09.80 SSV.dial :exhaust @15:54:10.56 Exhaust.select :open @15:54:10.67 SS.seek 0ml @15:54:11.03 PSV.dial :air @15:54:11.51 PS.seek 0ml @15:54:19.68 Exhaust.select :close @15:54:19.79 SSV.dial CBV @15:54:19.81 CBV.dial SSV @15:54:20.59 Allowing 130s for 25.0ml of remaining 875.0ml @15:54:20.61 Intake.select :open @15:54:22.76 Intake @16.6psia, vacuum from 10.0 to 13.0psi @15:54:22.78 SS.reconfigure :inhaling 0.87ml/s @15:54:22.82 SS.seek 25ml @15:54:34.80 washCycleDA #1 of 2 @15:54:34.81 PSR.select :air @15:54:34.82 PSV.dial PSR @15:54:36.20 Pulling 0.5ml of PSR.wash @15:54:36.21 PSR.select :wash @15:54:36.32 PS.reconfigure :default @15:54:36.36 PS.seek 0.5ml @15:54:42.33 delay 5 seconds @15:54:47.35 PSR.select :air @15:54:47.46 PS.seek 2ml @15:54:53.82 Vacuum too high -- waiting up to 40 seconds @15:54:53.83 SS.reconfigure :exhale @15:54:53.87 SS.hold @15:54:58.05 Intake @16.4psia, vacuum from 10.0 to 13.0psi @15:54:58.07 SS.reconfigure :inhaling 0.78ml/s @15:54:58.11 SS.seek 25ml @15:54:58.59 Stroke completed -- waiting for pressure to equalize ... @15:54:58.60 SS.reconfigure :exhale @15:54:59.14 SS.hold @15:55:02.72 Intake.select :close @15:55:02.86 SSV.dial :exhaust @15:55:03.63 Exhaust.select :open @15:55:03.69 PTV.dial :puck @15:55:03.74 SS.seek 0ml @15:55:04.44 PSV.dial PTV @15:55:05.19 PBV.dial :waste1 @15:55:05.62 PS.seek 1.5ml @15:55:11.64 delay 3 seconds @15:55:12.51 Exhaust.select :close @15:55:12.62 SSV.dial CBV @15:55:12.64 CBV.dial SSV @15:55:13.42 Allowing 130s for 25.0ml of remaining 850.0ml @15:55:13.43 Intake.select :open @15:55:14.66 PBV.dial between PSV and waste1 @15:55:15.02 PS.seek 0ml @15:55:15.59 Intake @16.5psia, vacuum from 10.0 to 13.0psi @15:55:15.61 SS.reconfigure :inhaling 0.78ml/s @15:55:15.66 SS.seek 25ml @15:55:31.17 delay 2:00 @15:55:50.21 Stroke completed -- waiting for pressure to equalize ... @15:55:50.22 SS.reconfigure :exhale @15:55:50.27 SS.hold @15:55:58.74 Intake.select :close @15:55:58.88 SSV.dial :exhaust @15:55:59.64 Exhaust.select :open @15:55:59.75 SS.seek 0ml @15:56:08.40 Exhaust.select :close @15:56:08.51 SSV.dial CBV @15:56:08.53 CBV.dial SSV @15:56:09.75 Allowing 130s for 25.0ml of remaining 825.0ml @15:56:09.76 Intake.select :open @15:56:11.91 Intake @16.4psia, vacuum from 10.0 to 13.0psi @15:56:11.93 SS.reconfigure :inhaling 0.78ml/s @15:56:11.97 SS.seek 25ml @15:56:46.59 Stroke completed -- waiting for pressure to equalize ... @15:56:46.60 SS.reconfigure :exhale @15:56:46.64 SS.hold @15:56:55.11 Intake.select :close @15:56:55.25 SSV.dial :exhaust @15:56:55.99 Exhaust.select :open @15:56:56.10 SS.seek 0ml @15:57:05.18 Exhaust.select :close @15:57:05.29 SSV.dial CBV @15:57:05.32 CBV.dial SSV @15:57:06.06 Allowing 130s for 25.0ml of remaining 800.0ml @15:57:06.07 Intake.select :open @15:57:08.23 Intake @16.5psia, vacuum from 10.0 to 13.0psi @15:57:08.25 SS.reconfigure :inhaling 0.78ml/s @15:57:08.29 SS.seek 25ml @15:57:31.20 recoverPSRreagent08 @15:57:31.21 PTV.dial :puck @15:57:31.22 PBV.dial :waste1 @15:57:31.58 delay 30 seconds @15:57:42.35 Stroke completed -- waiting for pressure to equalize ... @15:57:42.36 SS.reconfigure :exhale @15:57:42.40 SS.hold @15:57:52.29 Intake.select :close @15:57:52.43 SSV.dial :exhaust @15:57:53.19 Exhaust.select :open @15:57:53.30 SS.seek 0ml @15:58:01.60 PBV.dial between PSV and waste1 @15:58:01.95 PSR.select :air @15:58:01.97 PSV.dial PSR @15:58:02.08 Exhaust.select :close @15:58:02.19 SSV.dial CBV @15:58:02.21 CBV.dial SSV @15:58:02.74 PS.reconfigure :fast @15:58:02.78 PS.seek 2.5ml @15:58:02.99 Allowing 130s for 25.0ml of remaining 775.0ml @15:58:03.01 Intake.select :open @15:58:05.17 Intake @16.4psia, vacuum from 10.0 to 13.0psi @15:58:05.18 SS.reconfigure :inhaling 0.78ml/s @15:58:05.23 SS.seek 25ml @15:58:26.67 PSV.dial PTV @15:58:27.44 delay 5 seconds @15:58:32.45 PSV.dial PSR @15:58:33.33 delay 5 seconds @15:58:38.35 PSV.dial PTV @15:58:39.08 delay 5 seconds @15:58:39.75 Vacuum too high -- waiting up to 40 seconds @15:58:39.76 SS.reconfigure :exhale @15:58:39.80 SS.hold @15:58:44.09 PSV.dial :air @15:58:44.67 Intake @15.8psia, vacuum from 10.0 to 13.0psi @15:58:44.70 SS.reconfigure :inhaling 0.70ml/s @15:58:44.74 SS.seek 25ml @15:58:44.85 PS.seek 0ml @15:58:45.52 Stroke completed -- waiting for pressure to equalize ... @15:58:45.53 SS.reconfigure :exhale @15:58:45.58 SS.hold @15:58:49.67 Intake.select :close @15:58:49.82 SSV.dial :exhaust @15:58:50.58 Exhaust.select :open @15:58:50.70 SS.seek 0ml @15:58:59.72 Exhaust.select :close @15:58:59.83 SSV.dial CBV @15:58:59.85 CBV.dial SSV @15:59:00.60 Allowing 130s for 25.0ml of remaining 750.0ml @15:59:00.62 Intake.select :open @15:59:02.77 Intake @16.4psia, vacuum from 10.0 to 13.0psi @15:59:02.79 SS.reconfigure :inhaling 0.70ml/s @15:59:02.83 SS.seek 25ml @15:59:08.32 washCycleDA #2 of 2 @15:59:08.33 PSR.select :air @15:59:08.35 PSV.dial PSR @15:59:09.72 Pulling 0.5ml of PSR.wash @15:59:09.73 PSR.select :wash @15:59:09.85 PS.reconfigure :default @15:59:09.89 PS.seek 0.5ml @15:59:16.01 delay 5 seconds @15:59:21.03 PSR.select :air @15:59:21.15 PS.seek 2ml @15:59:37.35 PTV.dial :puck @15:59:37.37 PSV.dial PTV @15:59:38.10 PBV.dial :waste1 @15:59:38.53 PS.seek 1.5ml @15:59:40.10 Stroke completed -- waiting for pressure to equalize ... @15:59:40.11 SS.reconfigure :exhale @15:59:40.15 SS.hold @15:59:44.65 delay 3 seconds @15:59:47.68 PBV.dial between PSV and waste1 @15:59:48.05 PS.seek 0ml @15:59:49.15 Intake.select :close @15:59:49.30 SSV.dial :exhaust @15:59:50.04 Exhaust.select :open @15:59:50.15 SS.seek 0ml @15:59:58.94 Exhaust.select :close @15:59:59.05 SSV.dial CBV @15:59:59.07 CBV.dial SSV @15:59:59.85 Allowing 130s for 25.0ml of remaining 725.0ml @15:59:59.86 Intake.select :open @16:00:02.02 Intake @16.4psia, vacuum from 10.0 to 13.0psi @16:00:02.04 SS.reconfigure :inhaling 0.70ml/s @16:00:02.08 SS.seek 25ml @16:00:04.39 delay 2:00 @16:00:39.83 Stroke completed -- waiting for pressure to equalize ... @16:00:39.84 SS.reconfigure :exhale @16:00:39.89 SS.hold @16:00:49.58 Intake.select :close @16:00:49.72 SSV.dial :exhaust @16:00:50.49 Exhaust.select :open @16:00:50.60 SS.seek 0ml @16:00:59.35 Exhaust.select :close @16:00:59.46 SSV.dial CBV @16:00:59.47 CBV.dial SSV @16:01:00.26 Allowing 130s for 25.0ml of remaining 700.0ml @16:01:00.27 Intake.select :open @16:01:02.42 Intake @16.4psia, vacuum from 10.0 to 13.0psi @16:01:02.44 SS.reconfigure :inhaling 0.70ml/s @16:01:02.48 SS.seek 25ml @16:01:40.49 Stroke completed -- waiting for pressure to equalize ... @16:01:40.50 SS.reconfigure :exhale @16:01:40.54 SS.hold @16:01:50.22 Intake.select :close @16:01:50.37 SSV.dial :exhaust @16:01:51.13 Exhaust.select :open @16:01:51.24 SS.seek 0ml @16:02:00.13 Exhaust.select :close @16:02:00.24 SSV.dial CBV @16:02:00.29 CBV.dial SSV @16:02:01.02 Allowing 130s for 25.0ml of remaining 675.0ml @16:02:01.03 Intake.select :open @16:02:03.19 Intake @16.5psia, vacuum from 10.0 to 13.0psi @16:02:03.21 SS.reconfigure :inhaling 0.70ml/s @16:02:03.25 SS.seek 25ml @16:02:04.41 recoverPSRreagent08 @16:02:04.42 PTV.dial :puck @16:02:04.43 PBV.dial :waste1 @16:02:04.81 delay 30 seconds @16:02:34.83 PBV.dial between PSV and waste1 @16:02:35.20 PSR.select :air @16:02:35.21 PSV.dial PSR @16:02:35.98 PS.reconfigure :fast @16:02:36.03 PS.seek 2.5ml @16:02:37.40 Vacuum too high -- waiting up to 40 seconds @16:02:37.41 SS.reconfigure :exhale @16:02:37.46 SS.hold @16:02:41.71 Intake @15.7psia, vacuum from 10.0 to 13.0psi @16:02:41.73 SS.reconfigure :inhaling 0.63ml/s @16:02:41.77 SS.seek 25ml @16:02:46.39 Stroke completed -- waiting for pressure to equalize ... @16:02:46.40 SS.reconfigure :exhale @16:02:46.45 SS.hold @16:02:52.87 Intake.select :close @16:02:53.01 SSV.dial :exhaust @16:02:53.75 Exhaust.select :open @16:02:53.86 SS.seek 0ml @16:03:00.01 PSV.dial PTV @16:03:00.77 delay 5 seconds @16:03:02.67 Exhaust.select :close @16:03:02.78 SSV.dial CBV @16:03:02.80 CBV.dial SSV @16:03:03.57 Allowing 130s for 25.0ml of remaining 650.0ml @16:03:03.58 Intake.select :open @16:03:05.73 Intake @16.3psia, vacuum from 10.0 to 13.0psi @16:03:05.75 SS.reconfigure :inhaling 0.63ml/s @16:03:05.80 SS.seek 25ml @16:03:05.84 PSV.dial PSR @16:03:06.59 delay 5 seconds @16:03:11.60 PSV.dial PTV @16:03:12.35 delay 5 seconds @16:03:17.37 PSV.dial :air @16:03:18.25 PS.seek 0ml @16:03:41.57 getPMLReagent08 @16:03:41.58 PRV.dial :air @16:03:42.02 delay 5 seconds @16:03:47.04 PTV.dial :PRVmixing @16:03:47.89 Stroke completed -- waiting for pressure to equalize ... @16:03:47.90 SS.reconfigure :exhale @16:03:47.95 SS.hold @16:03:48.35 delay 5 seconds @16:03:53.44 PRV.dial :ab2 @16:03:53.89 PTV.dial :PRVmixing @16:03:53.91 PSV.dial :mixloop @16:03:54.36 PS.reconfigure :default @16:03:54.40 PS.seek 0.3ml @16:03:57.64 Intake.select :close @16:03:57.79 SSV.dial :exhaust @16:03:58.32 delay 2 seconds @16:03:58.53 Exhaust.select :open @16:03:58.65 SS.seek 0ml @16:04:00.33 PRV.dial :air @16:04:00.86 PS.seek 0.55ml @16:04:04.26 addPMLReagent08 @16:04:04.27 PSV.dial :air @16:04:04.73 PS.seek 2ml @16:04:07.50 Exhaust.select :close @16:04:07.61 SSV.dial CBV @16:04:07.64 CBV.dial SSV @16:04:08.38 Allowing 130s for 25.0ml of remaining 625.0ml @16:04:08.39 Intake.select :open @16:04:10.54 Intake @16.4psia, vacuum from 10.0 to 13.0psi @16:04:10.56 SS.reconfigure :inhaling 0.63ml/s @16:04:10.61 SS.seek 25ml @16:04:20.28 PSV.dial :PML @16:04:20.75 PTV.dial :mixing @16:04:21.50 PBV.dial :waste1 @16:04:21.94 PS.seek 1.85ml @16:04:24.36 PBV.dial between PSV and waste1 @16:04:24.81 PS.seek 0ml @16:04:44.53 delay 2 seconds @16:04:46.55 delay 8:00 @16:04:52.72 Stroke completed -- waiting for pressure to equalize ... @16:04:52.73 SS.reconfigure :exhale @16:04:52.77 SS.hold @16:05:02.96 Intake.select :close @16:05:03.11 SSV.dial :exhaust @16:05:03.86 Exhaust.select :open @16:05:03.97 SS.seek 0ml @16:05:12.75 Exhaust.select :close @16:05:12.85 SSV.dial CBV @16:05:12.91 CBV.dial SSV @16:05:13.64 Allowing 130s for 25.0ml of remaining 600.0ml @16:05:13.65 Intake.select :open @16:05:15.81 Intake @16.6psia, vacuum from 10.0 to 13.0psi @16:05:15.83 SS.reconfigure :inhaling 0.63ml/s @16:05:15.87 SS.seek 25ml @16:05:58.00 Stroke completed -- waiting for pressure to equalize ... @16:05:58.01 SS.reconfigure :exhale @16:05:58.06 SS.hold @16:06:09.24 Intake.select :close @16:06:09.38 SSV.dial :exhaust @16:06:10.15 Exhaust.select :open @16:06:10.26 SS.seek 0ml @16:06:19.17 Exhaust.select :close @16:06:19.27 SSV.dial CBV @16:06:19.29 CBV.dial SSV @16:06:20.06 Allowing 130s for 25.0ml of remaining 575.0ml @16:06:20.08 Intake.select :open @16:06:22.23 Intake @16.5psia, vacuum from 10.0 to 13.0psi @16:06:22.25 SS.reconfigure :inhaling 0.63ml/s @16:06:22.29 SS.seek 25ml @16:07:04.38 Stroke completed -- waiting for pressure to equalize ... @16:07:04.40 SS.reconfigure :exhale @16:07:04.44 SS.hold @16:07:17.01 Intake.select :close @16:07:17.16 SSV.dial :exhaust @16:07:17.92 Exhaust.select :open @16:07:18.03 SS.seek 0ml @16:07:26.79 Exhaust.select :close @16:07:26.90 SSV.dial CBV @16:07:26.92 CBV.dial SSV @16:07:27.70 Allowing 130s for 25.0ml of remaining 550.0ml @16:07:27.71 Intake.select :open @16:07:29.87 Intake @16.6psia, vacuum from 10.0 to 13.0psi @16:07:29.89 SS.reconfigure :inhaling 0.63ml/s @16:07:29.93 SS.seek 25ml @16:08:04.89 Vacuum too high -- waiting up to 40 seconds @16:08:04.91 SS.reconfigure :exhale @16:08:04.95 SS.hold @16:08:10.60 Intake @16.6psia, vacuum from 10.0 to 13.0psi @16:08:11.13 SS.reconfigure :inhaling 0.57ml/s @16:08:11.17 SS.seek 25ml @16:08:19.15 Stroke completed -- waiting for pressure to equalize ... @16:08:19.16 SS.reconfigure :exhale @16:08:19.21 SS.hold @16:08:26.59 Intake.select :close @16:08:26.74 SSV.dial :exhaust @16:08:27.49 Exhaust.select :open @16:08:27.60 SS.seek 0ml @16:08:36.37 Exhaust.select :close @16:08:36.48 SSV.dial CBV @16:08:36.52 CBV.dial SSV @16:08:37.25 Allowing 130s for 25.0ml of remaining 525.0ml @16:08:37.26 Intake.select :open @16:08:39.41 Intake @16.6psia, vacuum from 10.0 to 13.0psi @16:08:39.43 SS.reconfigure :inhaling 0.57ml/s @16:08:39.48 SS.seek 25ml @16:09:25.60 Stroke completed -- waiting for pressure to equalize ... @16:09:25.61 SS.reconfigure :exhale @16:09:25.66 SS.hold @16:09:38.23 Intake.select :close @16:09:38.37 SSV.dial :exhaust @16:09:39.13 Exhaust.select :open @16:09:39.25 SS.seek 0ml @16:09:47.94 Exhaust.select :close @16:09:48.05 SSV.dial CBV @16:09:48.07 CBV.dial SSV @16:09:48.85 Allowing 130s for 25.0ml of remaining 500.0ml @16:09:48.87 Intake.select :open @16:09:51.02 Intake @16.6psia, vacuum from 10.0 to 13.0psi @16:09:51.04 SS.reconfigure :inhaling 0.57ml/s @16:09:51.08 SS.seek 25ml @16:10:33.99 Vacuum too high -- waiting up to 40 seconds @16:10:34.00 SS.reconfigure :exhale @16:10:34.04 SS.hold @16:10:38.91 Intake @16.1psia, vacuum from 10.0 to 13.0psi @16:10:38.93 SS.reconfigure :inhaling 0.51ml/s @16:10:38.97 SS.seek 25ml @16:10:42.70 Stroke completed -- waiting for pressure to equalize ... @16:10:42.71 SS.reconfigure :exhale @16:10:42.75 SS.hold @16:10:50.14 Intake.select :close @16:10:50.28 SSV.dial :exhaust @16:10:51.05 Exhaust.select :open @16:10:51.16 SS.seek 0ml @16:10:59.99 Exhaust.select :close @16:11:00.10 SSV.dial CBV @16:11:00.65 CBV.dial SSV @16:11:01.40 Allowing 130s for 25.0ml of remaining 475.0ml @16:11:01.41 Intake.select :open @16:11:03.57 Intake @16.6psia, vacuum from 10.0 to 13.0psi @16:11:03.59 SS.reconfigure :inhaling 0.51ml/s @16:11:03.63 SS.seek 25ml @16:11:54.82 Stroke completed -- waiting for pressure to equalize ... @16:11:54.83 SS.reconfigure :exhale @16:11:54.87 SS.hold @16:12:05.94 Intake.select :close @16:12:06.08 SSV.dial :exhaust @16:12:06.85 Exhaust.select :open @16:12:06.96 SS.seek 0ml @16:12:15.80 Exhaust.select :close @16:12:15.91 SSV.dial CBV @16:12:15.93 CBV.dial SSV @16:12:16.71 Allowing 130s for 25.0ml of remaining 450.0ml @16:12:16.72 Intake.select :open @16:12:18.88 Intake @16.7psia, vacuum from 10.0 to 13.0psi @16:12:18.90 SS.reconfigure :inhaling 0.51ml/s @16:12:18.94 SS.seek 25ml @16:12:46.58 recoverPRVreagent08 @16:12:46.59 PTV.dial :mixing @16:12:46.60 PBV.dial :waste1 @16:12:46.96 delay 30 seconds @16:13:10.10 Stroke completed -- waiting for pressure to equalize ... @16:13:10.11 SS.reconfigure :exhale @16:13:10.15 SS.hold @16:13:16.98 PBV.dial between PSV and waste1 @16:13:17.35 PSV.dial :air @16:13:17.83 PS.reconfigure :fast @16:13:17.87 PS.seek 2.5ml @16:13:22.79 Intake.select :close @16:13:22.93 SSV.dial :exhaust @16:13:23.69 Exhaust.select :open @16:13:23.80 SS.seek 0ml @16:13:32.86 Exhaust.select :close @16:13:32.97 SSV.dial CBV @16:13:32.99 CBV.dial SSV @16:13:33.77 Allowing 130s for 25.0ml of remaining 425.0ml @16:13:33.78 Intake.select :open @16:13:35.94 Intake @16.7psia, vacuum from 10.0 to 13.0psi @16:13:35.96 SS.reconfigure :inhaling 0.51ml/s @16:13:36.00 SS.seek 25ml @16:13:41.71 PSV.dial :mixloop @16:13:42.18 delay 2 seconds @16:13:44.20 PSV.dial :air @16:13:44.67 delay 2 seconds @16:13:46.68 PSV.dial :mixloop @16:13:47.16 delay 2 seconds @16:13:49.17 PSV.dial :air @16:13:49.64 PS.seek 0ml @16:14:12.93 washCycleDA #1 of 3 @16:14:12.94 PSR.select :air @16:14:12.96 PSV.dial PSR @16:14:14.33 Pulling 0.5ml of PSR.wash @16:14:14.34 PSR.select :wash @16:14:14.45 PS.reconfigure :default @16:14:14.49 PS.seek 0.5ml @16:14:20.53 delay 5 seconds @16:14:25.54 PSR.select :air @16:14:25.66 PS.seek 2ml @16:14:25.89 Vacuum too high -- waiting up to 40 seconds @16:14:25.90 SS.reconfigure :exhale @16:14:25.94 SS.hold @16:14:31.73 Intake @16.5psia, vacuum from 10.0 to 13.0psi @16:14:31.75 SS.reconfigure :inhaling 0.47ml/s @16:14:31.79 SS.seek 25ml @16:14:32.84 Stroke completed -- waiting for pressure to equalize ... @16:14:32.86 SS.reconfigure :exhale @16:14:32.90 SS.hold @16:14:40.29 Intake.select :close @16:14:40.43 SSV.dial :exhaust @16:14:41.19 Exhaust.select :open @16:14:41.30 SS.seek 0ml @16:14:41.87 PTV.dial :puck @16:14:42.62 PSV.dial PTV @16:14:43.37 PBV.dial :waste1 @16:14:43.80 PS.seek 1.5ml @16:14:49.80 delay 3 seconds @16:14:50.34 Exhaust.select :close @16:14:50.45 SSV.dial CBV @16:14:50.48 CBV.dial SSV @16:14:51.25 Allowing 130s for 25.0ml of remaining 400.0ml @16:14:51.27 Intake.select :open @16:14:52.82 PBV.dial between PSV and waste1 @16:14:53.20 PS.seek 0ml @16:14:53.42 Intake @16.8psia, vacuum from 10.0 to 13.0psi @16:14:53.44 SS.reconfigure :inhaling 0.47ml/s @16:14:53.49 SS.seek 25ml @16:15:09.31 delay 2:00 @16:15:49.01 Stroke completed -- waiting for pressure to equalize ... @16:15:49.03 SS.reconfigure :exhale @16:15:49.07 SS.hold @16:16:01.68 Intake.select :close @16:16:01.82 SSV.dial :exhaust @16:16:02.56 Exhaust.select :open @16:16:02.67 SS.seek 0ml @16:16:11.47 Exhaust.select :close @16:16:11.58 SSV.dial CBV @16:16:11.60 CBV.dial SSV @16:16:12.38 Allowing 130s for 25.0ml of remaining 375.0ml @16:16:12.39 Intake.select :open @16:16:14.54 Intake @16.8psia, vacuum from 10.0 to 13.0psi @16:16:14.56 SS.reconfigure :inhaling 0.47ml/s @16:16:14.61 SS.seek 25ml @16:17:09.33 recoverPSRreagent08 @16:17:09.34 PTV.dial :puck @16:17:09.35 PBV.dial :waste1 @16:17:09.71 delay 30 seconds @16:17:10.36 Stroke completed -- waiting for pressure to equalize ... @16:17:10.37 SS.reconfigure :exhale @16:17:10.41 SS.hold @16:17:23.01 Intake.select :close @16:17:23.67 SSV.dial :exhaust @16:17:24.44 Exhaust.select :open @16:17:24.55 SS.seek 0ml @16:17:33.37 Exhaust.select :close @16:17:33.48 SSV.dial CBV @16:17:33.50 CBV.dial SSV @16:17:34.28 Allowing 130s for 25.0ml of remaining 350.0ml @16:17:34.30 Intake.select :open @16:17:36.45 Intake @16.8psia, vacuum from 10.0 to 13.0psi @16:17:36.47 SS.reconfigure :inhaling 0.47ml/s @16:17:36.52 SS.seek 25ml @16:17:39.72 PBV.dial between PSV and waste1 @16:17:40.10 PSR.select :air @16:17:40.12 PSV.dial PSR @16:17:40.89 PS.reconfigure :fast @16:17:40.93 PS.seek 2.5ml @16:18:04.61 PSV.dial PTV @16:18:05.38 delay 5 seconds @16:18:10.40 PSV.dial PSR @16:18:11.16 delay 5 seconds @16:18:16.18 PSV.dial PTV @16:18:16.94 delay 5 seconds @16:18:21.96 PSV.dial :air @16:18:22.73 PS.seek 0ml @16:18:27.07 Vacuum too high -- waiting up to 40 seconds @16:18:27.09 SS.reconfigure :exhale @16:18:27.13 SS.hold @16:18:33.56 Intake @17.2psia, vacuum from 10.0 to 13.0psi @16:18:33.58 SS.reconfigure :inhaling 0.43ml/s @16:18:33.63 SS.seek 25ml @16:18:39.71 Stroke completed -- waiting for pressure to equalize ... @16:18:39.73 SS.reconfigure :exhale @16:18:39.77 SS.hold @16:18:46.05 washCycleDA #2 of 3 @16:18:46.06 PSR.select :air @16:18:46.08 PSV.dial PSR @16:18:47.44 Pulling 0.5ml of PSR.wash @16:18:47.45 PSR.select :wash @16:18:47.57 PS.reconfigure :default @16:18:47.62 PS.seek 0.5ml @16:18:48.33 Intake.select :close @16:18:48.48 SSV.dial :exhaust @16:18:49.23 Exhaust.select :open @16:18:49.34 SS.seek 0ml @16:18:53.58 delay 5 seconds @16:18:58.17 Exhaust.select :close @16:18:58.27 SSV.dial CBV @16:18:58.32 CBV.dial SSV @16:18:58.60 PSR.select :air @16:18:58.72 PS.seek 2ml @16:18:59.05 Allowing 130s for 25.0ml of remaining 325.0ml @16:18:59.06 Intake.select :open @16:19:01.21 Intake @16.9psia, vacuum from 10.0 to 13.0psi @16:19:01.23 SS.reconfigure :inhaling 0.43ml/s @16:19:01.27 SS.seek 25ml @16:19:15.02 PTV.dial :puck @16:19:15.03 PSV.dial PTV @16:19:15.75 PBV.dial :waste1 @16:19:16.19 PS.seek 1.5ml @16:19:22.05 delay 3 seconds @16:19:25.08 PBV.dial between PSV and waste1 @16:19:25.48 PS.seek 0ml @16:19:41.61 delay 2:00 @16:20:02.98 Stroke completed -- waiting for pressure to equalize ... @16:20:02.99 SS.reconfigure :exhale @16:20:03.03 SS.hold @16:20:17.32 Intake.select :close @16:20:17.47 SSV.dial :exhaust @16:20:18.23 Exhaust.select :open @16:20:18.34 SS.seek 0ml @16:20:27.10 Exhaust.select :close @16:20:27.21 SSV.dial CBV @16:20:27.23 CBV.dial SSV @16:20:28.01 Allowing 130s for 25.0ml of remaining 300.0ml @16:20:28.03 Intake.select :open @16:20:30.18 Intake @16.9psia, vacuum from 10.0 to 13.0psi @16:20:30.20 SS.reconfigure :inhaling 0.43ml/s @16:20:30.24 SS.seek 25ml @16:21:32.10 Stroke completed -- waiting for pressure to equalize ... @16:21:32.11 SS.reconfigure :exhale @16:21:32.16 SS.hold @16:21:41.63 recoverPSRreagent08 @16:21:41.64 PTV.dial :puck @16:21:41.65 PBV.dial :waste1 @16:21:42.01 delay 30 seconds @16:21:46.44 Intake.select :close @16:21:46.58 SSV.dial :exhaust @16:21:47.32 Exhaust.select :open @16:21:47.43 SS.seek 0ml @16:21:56.34 Exhaust.select :close @16:21:56.44 SSV.dial CBV @16:21:56.47 CBV.dial SSV @16:21:57.23 Allowing 130s for 25.0ml of remaining 275.0ml @16:21:57.24 Intake.select :open @16:21:59.40 Intake @17.0psia, vacuum from 10.0 to 13.0psi @16:21:59.42 SS.reconfigure :inhaling 0.43ml/s @16:21:59.46 SS.seek 25ml @16:22:12.04 PBV.dial between PSV and waste1 @16:22:12.39 PSR.select :air @16:22:12.41 PSV.dial PSR @16:22:13.18 PS.reconfigure :fast @16:22:13.22 PS.seek 2.5ml @16:22:36.98 PSV.dial PTV @16:22:37.75 delay 5 seconds @16:22:42.77 PSV.dial PSR @16:22:43.53 delay 5 seconds @16:22:48.55 PSV.dial PTV @16:22:49.31 delay 5 seconds @16:22:53.88 Vacuum too high -- waiting up to 40 seconds @16:22:53.90 SS.reconfigure :exhale @16:22:53.94 SS.hold @16:22:54.33 PSV.dial :air @16:22:55.21 PS.seek 0ml @16:22:59.51 Intake @16.7psia, vacuum from 10.0 to 13.0psi @16:22:59.53 SS.reconfigure :inhaling 0.38ml/s @16:22:59.57 SS.seek 25ml @16:23:08.01 Stroke completed -- waiting for pressure to equalize ... @16:23:08.02 SS.reconfigure :exhale @16:23:08.06 SS.hold @16:23:18.52 washCycleDA #3 of 3 @16:23:18.53 PSR.select :air @16:23:18.55 PSV.dial PSR @16:23:19.14 Intake.select :close @16:23:19.28 SSV.dial :exhaust @16:23:19.94 Pulling 0.5ml of PSR.wash @16:23:19.95 PSR.select :wash @16:23:20.04 Exhaust.select :open @16:23:20.10 PS.reconfigure :default @16:23:20.16 PS.seek 0.5ml @16:23:20.18 SS.seek 0ml @16:23:26.08 delay 5 seconds @16:23:28.99 Exhaust.select :close @16:23:29.10 SSV.dial CBV @16:23:29.12 CBV.dial SSV @16:23:29.90 Allowing 130s for 25.0ml of remaining 250.0ml @16:23:29.91 Intake.select :open @16:23:31.09 PSR.select :air @16:23:31.21 PS.seek 2ml @16:23:32.07 Intake @17.0psia, vacuum from 10.0 to 13.0psi @16:23:32.09 SS.reconfigure :inhaling 0.38ml/s @16:23:32.13 SS.seek 25ml @16:23:47.42 PTV.dial :puck @16:23:47.43 PSV.dial PTV @16:23:48.18 PBV.dial :waste1 @16:23:48.60 PS.seek 1.5ml @16:23:54.51 delay 3 seconds @16:23:57.53 PBV.dial between PSV and waste1 @16:23:57.90 PS.seek 0ml @16:24:14.23 delay 2:00 @16:24:41.19 Stroke completed -- waiting for pressure to equalize ... @16:24:41.20 SS.reconfigure :exhale @16:24:41.25 SS.hold @16:24:55.54 Intake.select :close @16:24:55.68 SSV.dial :exhaust @16:24:56.44 Exhaust.select :open @16:24:56.55 SS.seek 0ml @16:25:05.44 Exhaust.select :close @16:25:05.55 SSV.dial CBV @16:25:05.57 CBV.dial SSV @16:25:06.35 Allowing 130s for 25.0ml of remaining 225.0ml @16:25:06.36 Intake.select :open @16:25:08.52 Intake @16.9psia, vacuum from 10.0 to 13.0psi @16:25:08.54 SS.reconfigure :inhaling 0.38ml/s @16:25:08.58 SS.seek 25ml @16:26:14.25 recoverPSRreagent08 @16:26:14.26 PTV.dial :puck @16:26:14.27 PBV.dial :waste1 @16:26:14.62 delay 30 seconds @16:26:18.58 Stroke completed -- waiting for pressure to equalize ... @16:26:18.59 SS.reconfigure :exhale @16:26:18.63 SS.hold @16:26:32.92 Intake.select :close @16:26:33.06 SSV.dial :exhaust @16:26:33.82 Exhaust.select :open @16:26:33.93 SS.seek 0ml @16:26:42.78 Exhaust.select :close @16:26:42.89 SSV.dial CBV @16:26:42.91 CBV.dial SSV @16:26:43.69 Allowing 130s for 25.0ml of remaining 200.0ml @16:26:43.70 Intake.select :open @16:26:44.64 PBV.dial between PSV and waste1 @16:26:44.99 PSR.select :air @16:26:45.01 PSV.dial PSR @16:26:45.86 Intake @16.9psia, vacuum from 10.0 to 13.0psi @16:26:45.88 SS.reconfigure :inhaling 0.38ml/s @16:26:45.92 SS.seek 25ml @16:26:46.30 PS.reconfigure :fast @16:26:46.34 PS.seek 2.5ml @16:27:10.01 PSV.dial PTV @16:27:10.78 delay 5 seconds @16:27:15.80 PSV.dial PSR @16:27:16.56 delay 5 seconds @16:27:21.58 PSV.dial PTV @16:27:22.34 delay 5 seconds @16:27:27.36 PSV.dial :air @16:27:28.23 PS.seek 0ml @16:27:51.47 Gate.power :camera,:ON @16:27:51.49 Mixing DA Substrates @16:27:51.50 PSR.select :air @16:27:51.51 PSV.dial PSR @16:27:53.04 Pulling 0.25ml of PSR.sub1 @16:27:53.07 PSR.select :sub1 @16:27:53.27 PS.reconfigure :default @16:27:53.38 PS.seek 0.25ml @16:27:55.83 Stroke completed -- waiting for pressure to equalize ... @16:27:55.84 SS.reconfigure :exhale @16:27:55.88 SS.hold @16:27:56.82 delay 5 seconds @16:28:01.89 Pulling 0.25ml of PSR.sub2 @16:28:01.90 PSR.select :sub2 @16:28:02.01 PS.seek 0.5ml @16:28:05.35 delay 5 seconds @16:28:10.36 PSR.select :air @16:28:10.48 PS.seek 2ml @16:28:14.17 Intake.select :close @16:28:14.31 SSV.dial :exhaust @16:28:15.08 Exhaust.select :open @16:28:15.19 SS.seek 0ml @16:28:24.09 Exhaust.select :close @16:28:24.20 SSV.dial CBV @16:28:24.22 CBV.dial SSV @16:28:25.02 Allowing 130s for 25.0ml of remaining 174.9ml @16:28:25.03 Intake.select :open @16:28:26.79 PTV.dial :puck @16:28:26.80 PSV.dial PTV @16:28:27.19 Intake @17.0psia, vacuum from 10.0 to 13.0psi @16:28:27.21 SS.reconfigure :inhaling 0.38ml/s @16:28:27.25 SS.seek 25ml @16:28:27.57 PBV.dial :waste1 @16:28:28.01 PS.seek 1.5ml @16:28:33.94 delay 3 seconds @16:28:36.96 PBV.dial between PSV and waste1 @16:28:37.34 PS.seek 0ml @16:28:53.57 delay 10 seconds @16:29:03.60 recoverPSRreagent08 @16:29:03.61 PTV.dial :puck @16:29:03.62 PBV.dial :waste1 @16:29:04.00 delay 30 seconds @16:29:34.03 PBV.dial between PSV and waste1 @16:29:34.40 PSR.select :air @16:29:34.41 PSV.dial PSR @16:29:35.18 PS.reconfigure :fast @16:29:35.23 PS.seek 2.5ml @16:29:36.64 Stroke completed -- waiting for pressure to equalize ... @16:29:36.65 SS.reconfigure :exhale @16:29:36.69 SS.hold @16:29:56.97 Intake.select :close @16:29:57.11 SSV.dial :exhaust @16:29:58.39 Exhaust.select :open @16:29:58.50 SS.seek 0ml @16:29:59.28 PSV.dial PTV @16:30:00.18 delay 5 seconds @16:30:05.20 PSV.dial PSR @16:30:05.94 delay 5 seconds @16:30:07.37 Exhaust.select :close @16:30:07.48 SSV.dial CBV @16:30:07.50 CBV.dial SSV @16:30:08.28 Allowing 130s for 25.0ml of remaining 149.9ml @16:30:08.29 Intake.select :open @16:30:10.46 Intake @17.1psia, vacuum from 10.0 to 13.0psi @16:30:10.48 SS.reconfigure :inhaling 0.38ml/s @16:30:10.52 SS.seek 25ml @16:30:10.96 PSV.dial PTV @16:30:11.83 delay 5 seconds @16:30:16.85 PSV.dial :air @16:30:17.64 PS.seek 0ml @16:30:41.29 Additional air clear @16:30:41.30 PSR.select :air @16:30:41.31 PSV.dial PSR @16:30:42.68 Pulling 2ml of PSR.air @16:30:42.69 PSR.select :air @16:30:42.72 PS.reconfigure :default @16:30:42.76 PS.seek 2ml @16:31:03.94 delay 5 seconds @16:31:08.96 PTV.dial :puck @16:31:08.98 PSV.dial PTV @16:31:09.21 Vacuum too high -- waiting up to 40 seconds @16:31:09.22 SS.reconfigure :exhale @16:31:09.26 SS.hold @16:31:09.84 PBV.dial :waste1 @16:31:10.27 PS.seek 1.5ml @16:31:16.28 delay 3 seconds @16:31:17.75 Intake @18.2psia, vacuum from 10.0 to 13.0psi @16:31:17.76 SS.reconfigure :inhaling 0.34ml/s @16:31:17.81 SS.seek 25ml @16:31:19.30 PBV.dial between PSV and waste1 @16:31:19.68 delay 1 second @16:31:20.70 PSV.dial :waste1 @16:31:21.74 PS.seek 0ml @16:31:30.37 Stroke completed -- waiting for pressure to equalize ... @16:31:30.38 SS.reconfigure :exhale @16:31:30.43 SS.hold @16:31:37.86 Processing Puck Heater at 28C before imaging @16:31:37.87 PH.coast @16:31:38.00 Imaging da puck in Processing Clamp while storing it to tube 3 @16:31:38.17 SC.reconfigure :tight @16:31:38.21 SC.seek 3 @16:31:41.79 SC.configure :limp @16:31:41.99 Elbow.reconfigure :tight @16:31:42.03 Elbow.seek 3 @16:31:43.79 Forearm.reconfigure :tight @16:31:43.83 Forearm.seek 3 + 450 counts @16:31:44.84 SE.reconfigure :pick @16:31:44.90 SE.seek :beamBreak @16:31:46.65 Intake.select :close @16:31:46.79 SSV.dial :exhaust @16:31:46.98 SE.configure :default @16:31:47.13 Elbow.reconfigure :limp @16:31:47.18 Forearm.reconfigure :loose @16:31:47.22 Forearm.seek 3 @16:31:47.47 Elbow.hold @16:31:47.53 Elbow.reconfigure :tight @16:31:47.60 Exhaust.select :open @16:31:48.24 SE.seek 15.13 Pucks below Top Plate @16:31:48.30 SS.seek 0ml @16:31:48.68 Forearm.force -20 @16:31:48.72 Forearm.seek 3 + 307 counts @16:31:49.02 Forearm.reconfigure :tight @16:31:49.06 Forearm.seek 3 + 450 counts @16:31:49.29 SE.seek 16.13 Pucks below Top Plate @16:31:49.82 Forearm.reconfigure :loose @16:31:49.86 Forearm.seek :retract @16:31:50.79 Elbow.seek PC @16:31:53.45 Elbow.reconfigure :loose @16:31:53.54 Forearm.seek PC @16:31:54.52 Hand.close @16:31:55.11 PC.seek :open @16:31:57.12 Exhaust.select :close @16:31:57.23 SSV.dial CBV @16:31:57.25 CBV.dial SSV @16:31:58.03 Allowing 130s for 25.0ml of remaining 124.9ml @16:31:58.04 Intake.select :open @16:31:59.92 Forearm.seek :retract @16:32:00.20 Intake @17.2psia, vacuum from 10.0 to 13.0psi @16:32:00.22 SS.reconfigure :inhaling 0.34ml/s @16:32:00.26 SS.seek 25ml @16:32:00.88 Elbow.reconfigure :tight @16:32:00.92 Elbow.seek 3 @16:32:03.63 Forearm.reconfigure :tight @16:32:03.68 Forearm.seek 3 + 450 counts @16:32:04.64 Hand.open @16:32:05.30 SE.seek 15.78 Pucks below Top Plate @16:32:05.58 SE.reconfigure :seal @16:32:05.62 SE.seek 14.82 Pucks below Top Plate @16:32:06.12 Lights, camera, ACTION! @16:32:06.16 PuckCamera: snap -bin=2,2 -auto /var/log/esp/da23mar1016h1000ml.tif @16:32:25.67 Waiting >20 seconds for da to relinquish FlushPuck @16:32:32.43 SX HX9/16 (USB:21): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 22.949 seconds /var/log/esp/da23mar1016h1000ml.tif: TIFF Upload Complete @16:32:32.45 PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da23mar1016h1000ml15s.tif @16:32:48.07 SX HX9/16 (USB:21): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15 seconds /var/log/esp/midres/da23mar1016h1000ml15s.tif: TIFF Upload Complete @16:32:48.09 PuckCamera: snap -bin=2,2 20.0 /var/log/esp/midres/da23mar1016h1000ml20s.tif @16:33:08.75 SX HX9/16 (USB:21): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 20 seconds /var/log/esp/midres/da23mar1016h1000ml20s.tif: TIFF Upload Complete @16:33:08.77 Gate.power :camera,:OFF @16:33:08.78 DAfans.email "22.949 sec auto-exposure",Subject: "da23mar1016h1000ml images!" @16:33:09.04 Elbow.seek 3 @16:33:09.22 Forearm.seek 3 + 450 counts @16:33:09.35 SE.reconfigure :gently @16:33:09.40 SE.seek 15.54 Pucks below Top Plate @16:33:10.14 Elbow.reconfigure :limp @16:33:10.19 Forearm.reconfigure :loose @16:33:10.27 Forearm.seek 3 @16:33:10.53 Elbow.hold @16:33:10.61 Elbow.reconfigure :tight @16:33:10.67 SE.reconfigure :default @16:33:10.71 SE.seek 15.72 Pucks below Top Plate @16:33:11.25 Forearm.force -20 @16:33:11.29 Forearm.seek 3 + 303 counts @16:33:11.60 Forearm.reconfigure :tight @16:33:11.64 Forearm.seek 3 + 450 counts @16:33:11.94 SE.seek :down @16:33:13.27 Forearm.reconfigure :loose @16:33:13.36 Forearm.seek :retract @16:33:13.60 SE.stop @16:33:14.53 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @16:33:14.92 Elbow.seek FlushPuck::Garage @16:33:18.08 Elbow.reconfigure :loose @16:33:18.28 Forearm.seek :garage @16:33:19.35 Hand.close @16:33:20.18 Stroke completed -- waiting for pressure to equalize ... @16:33:20.31 SS.reconfigure :exhale @16:33:20.41 SS.hold @16:33:20.59 Forearm.seek :retract @16:33:21.67 Elbow.reconfigure :tight @16:33:21.75 Elbow.seek PC @16:33:23.49 Elbow.reconfigure :loose @16:33:23.87 Forearm.reconfigure :tight @16:33:23.94 Forearm.seek PC @16:33:25.60 PC.seek :closed @16:33:30.40 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.62 @16:33:30.56 Hand.open @16:33:31.38 Forearm.reconfigure :loose @16:33:31.47 Forearm.seek :retract @16:33:32.46 PSR.select :air @16:33:32.49 PSV.dial PSR @16:33:33.13 Pulling 0.5ml of PSR.flush @16:33:33.15 PSR.select :flush @16:33:33.32 PS.seek 0.5ml @16:33:39.19 Intake.select :close @16:33:39.41 delay 5 seconds @16:33:39.55 SSV.dial :exhaust @16:33:40.37 Exhaust.select :open @16:33:40.56 SS.seek 0ml @16:33:44.44 PSR.select :air @16:33:44.60 PS.seek 2.5ml @16:33:47.93 upsync @16:33:49.34 Exhaust.select :close @16:33:49.45 SSV.dial CBV @16:33:49.47 CBV.dial SSV @16:33:50.23 Allowing 130s for 25.0ml of remaining 99.9ml @16:33:50.24 Intake.select :open @16:33:52.40 Intake @17.2psia, vacuum from 10.0 to 13.0psi @16:33:52.42 SS.reconfigure :inhaling 0.34ml/s @16:33:52.46 SS.seek 25ml @16:34:05.87 delay 1 second @16:34:06.89 PRV.dial :waste2,avoiding: :ab2 @16:34:08.75 PTV.dial :PRVmixing @16:34:10.06 PSV.dial :mixloop @16:34:11.11 PS.seek :empty @16:34:37.46 PSR.select :air @16:34:37.48 PSV.dial PSR @16:34:38.71 Pulling 0.5ml of PSR.flush @16:34:38.72 PSR.select :flush @16:34:38.83 PS.seek 0.5ml @16:34:44.97 delay 5 seconds @16:34:49.99 PSR.select :air @16:34:50.10 PS.seek 2.5ml @16:35:11.34 delay 1 second @16:35:12.36 PBV.dial :waste1 @16:35:12.41 Stroke completed -- waiting for pressure to equalize ... @16:35:12.44 SS.reconfigure :exhale @16:35:12.48 SS.hold @16:35:12.79 PTV.dial :mixing @16:35:13.59 PSV.dial :mixloop @16:35:14.63 PS.seek :empty @16:35:32.91 Intake.select :close @16:35:33.06 SSV.dial :exhaust @16:35:33.80 Exhaust.select :open @16:35:33.92 SS.seek 0ml @16:35:40.94 PSV.dial PSR @16:35:41.98 PSR.select :flush @16:35:42.10 PS.seek 1ml @16:35:42.94 Exhaust.select :close @16:35:43.05 SSV.dial CBV @16:35:43.07 CBV.dial SSV @16:35:43.84 Allowing 130s for 25.0ml of remaining 74.9ml @16:35:43.85 Intake.select :open @16:35:46.01 Intake @17.2psia, vacuum from 10.0 to 13.0psi @16:35:46.03 SS.reconfigure :inhaling 0.34ml/s @16:35:46.08 SS.seek 25ml @16:35:53.21 PSR.select :air @16:35:53.33 PS.seek 2.5ml @16:36:09.50 PSV.dial PTV @16:36:10.25 PTV.dial :puck @16:36:10.98 PBV.dial :waste1 @16:36:11.00 PS.seek 0ml @16:36:37.53 PSV.dial PSR @16:36:38.28 PSR.select :flush @16:36:38.40 PS.seek 0.5ml @16:36:44.37 PSR.select :air @16:36:44.48 PS.seek 2.5ml @16:37:05.68 PSV.dial PTV @16:37:06.02 Stroke completed -- waiting for pressure to equalize ... @16:37:06.03 SS.reconfigure :exhale @16:37:06.07 SS.hold @16:37:06.61 PBV.dial PSV @16:37:07.20 PS.seek 0ml @16:37:28.47 Intake.select :close @16:37:28.62 SSV.dial :exhaust @16:37:29.38 Exhaust.select :open @16:37:29.49 SS.seek 0ml @16:37:33.56 PSV.dial PBV @16:37:34.02 PS.seek 2.5ml @16:37:38.58 Exhaust.select :close @16:37:38.69 SSV.dial CBV @16:37:38.71 CBV.dial SSV @16:37:39.47 Allowing 130s for 25.0ml of remaining 49.9ml @16:37:39.49 Intake.select :open @16:37:41.65 Intake @17.2psia, vacuum from 10.0 to 13.0psi @16:37:41.67 SS.reconfigure :inhaling 0.34ml/s @16:37:41.71 SS.seek 25ml @16:38:00.40 PSV.dial :waste1 @16:38:01.14 PS.seek 0ml @16:38:27.68 PSV.dial PSR @16:38:28.15 PSR.select :air @16:38:28.17 PS.seek 2.5ml @16:38:54.55 PSV.dial PTV @16:38:55.29 PBV.dial PSV @16:38:55.31 PS.seek 0ml @16:39:01.44 Stroke completed -- waiting for pressure to equalize ... @16:39:01.45 SS.reconfigure :exhale @16:39:01.49 SS.hold @16:39:21.62 PSV.dial PBV @16:39:22.09 PS.seek 2.5ml @16:39:24.09 Intake.select :close @16:39:24.23 SSV.dial :exhaust @16:39:24.97 Exhaust.select :open @16:39:25.08 SS.seek 0ml @16:39:33.88 Exhaust.select :close @16:39:33.99 SSV.dial CBV @16:39:34.00 CBV.dial SSV @16:39:34.77 Allowing 130s for 24.9ml of remaining 24.9ml @16:39:34.78 Intake.select :open @16:39:36.94 Intake @17.2psia, vacuum from 10.0 to 13.0psi @16:39:36.96 SS.reconfigure :inhaling 0.34ml/s @16:39:37.00 SS.seek 24.95ml @16:39:48.49 PSV.dial :waste1 @16:39:49.23 PS.seek 0ml @16:40:15.68 PSV.dial PSR @16:40:16.15 PSR.select :flush @16:40:16.26 PS.seek 1ml @16:40:27.36 PSR.select :air @16:40:27.48 PS.seek 2.5ml @16:40:39.20 Vacuum too high -- waiting up to 40 seconds @16:40:39.21 SS.reconfigure :exhale @16:40:39.25 SS.hold @16:40:43.68 PSV.dial PTV @16:40:44.42 PTV.dial :puck @16:40:44.43 PBV.dial :waste1 @16:40:45.00 PS.seek 0ml @16:40:47.83 Intake @17.5psia, vacuum from 10.0 to 13.0psi @16:40:47.85 SS.reconfigure :inhaling 0.31ml/s @16:40:47.89 SS.seek 24.95ml @16:41:07.26 Stroke completed -- waiting for pressure to equalize ... @16:41:07.28 SS.reconfigure :exhale @16:41:07.32 SS.hold @16:41:11.39 PSV.dial PSR @16:41:12.15 PSR.select :flush @16:41:12.26 PS.seek 0.5ml @16:41:18.14 PSR.select :air @16:41:18.34 PS.seek 2.5ml @16:41:23.56 Intake.select :close @16:41:23.70 SSV.dial :exhaust @16:41:24.45 Exhaust.select :open @16:41:24.56 SS.seek 0ml @16:41:33.59 Exhaust.select :close @16:41:33.70 Sampled 1000.0ml @16:41:33.72 Drying puck before fixing for 20:00 @16:41:33.73 CBV.dial :waste2b @16:41:34.97 delay 1:00 @16:41:39.74 PSV.dial PTV @16:41:40.49 PBV.dial PSV @16:41:41.17 PS.seek 0ml @16:42:07.71 PSV.dial PBV @16:42:08.18 PS.seek 2.5ml @16:42:34.58 PSV.dial :waste1 @16:42:34.98 CSV.dial :air @16:42:35.32 PS.seek 0ml @16:42:35.44 CS.reconfigure :fast @16:42:35.49 CS.seek 10ml @16:42:59.78 Pumping wcr puck from tube 4 up to 35psi... @16:42:59.80 CBV.dial SSV @16:43:01.32 CBV.dial between CSV and waste2b @16:43:01.64 PSV.dial PSR @16:43:02.11 PSR.select :air @16:43:02.14 PS.seek 2.5ml @16:43:02.26 CSV.dial between air and CTV @16:43:02.59 CS.seek 6.869ml @16:43:10.60 CTV.dial CSV,avoiding: :air @16:43:10.62 CSV.dial CTV @16:43:11.41 CS.reconfigure :pressurize @16:43:11.46 CS.seek 0ml @16:43:23.23 CBV.dial :waste2b @16:43:23.56 CS.reconfigure :default @16:43:23.61 CS.seek 0ml @16:43:28.49 PSV.dial PTV @16:43:29.22 PBV.dial PSV @16:43:29.24 PS.seek 0ml @16:43:30.56 delay 1:00 @16:43:55.58 PSV.dial PBV @16:43:56.06 PS.seek 2.5ml @16:44:22.48 PSV.dial :waste1 @16:44:23.23 PS.seek 0ml @16:44:30.58 CTV.dial :air,avoiding: :intake @16:44:31.91 Fix #1 of 2 (with RNAlater) for 10:00 @16:44:31.92 CSV.dial CSR @16:44:32.41 Pulling 2ml of CSR.RNAlater @16:44:32.42 CSR.select :RNAlater @16:44:32.53 CS.seek 2ml @16:44:38.68 delay 5 seconds @16:44:43.70 CSR.select :air @16:44:43.81 CS.reconfigure :fast @16:44:43.87 CS.seek 10ml @16:44:49.55 Garaging FlushPuck in Processing Clamp @16:44:49.62 Elbow.reconfigure :tight @16:44:49.67 Elbow.seek PC @16:44:49.78 Elbow.reconfigure :loose @16:44:49.87 Forearm.seek PC @16:44:50.83 Hand.close @16:44:51.48 PC.seek :open @16:44:56.19 Forearm.seek :retract @16:44:57.13 Elbow.reconfigure :tight @16:44:57.18 Elbow.seek :garage @16:44:58.50 Elbow.reconfigure :loose @16:44:58.59 Forearm.reconfigure :tight @16:44:58.64 Forearm.seek :garage @16:44:59.65 Hand.open @16:45:00.29 Forearm.reconfigure :loose @16:45:00.33 Forearm.seek :retract @16:45:03.21 Pumping wcr puck from tube 4 up to 35psi... @16:45:03.23 CBV.dial SSV @16:45:04.53 CBV.dial between CSV and waste2b @16:45:05.48 CSV.dial between air and CTV @16:45:06.08 CS.seek 6.839ml @16:45:13.97 CTV.dial CSV,avoiding: :air @16:45:13.99 CSV.dial CTV @16:45:15.38 CS.reconfigure :pressurize @16:45:15.42 CS.seek 0ml @16:45:33.95 CSV.dial :air @16:45:34.44 CS.reconfigure :fast @16:45:34.49 CS.seek 10ml @16:45:58.46 CSV.dial between air and CTV @16:45:58.79 CS.seek 4.082ml @16:46:12.97 CTV.dial CSV,avoiding: :air @16:46:12.99 CSV.dial CTV @16:46:13.30 CS.reconfigure :pressurize @16:46:13.35 CS.seek 0ml @16:46:24.27 CSV.dial :air @16:46:24.73 CS.reconfigure :fast @16:46:24.78 CS.seek 10ml @16:46:48.80 CSV.dial between air and CTV @16:46:49.13 CS.seek 5.592ml @16:46:59.75 CTV.dial CSV,avoiding: :air @16:46:59.77 CSV.dial CTV @16:47:00.08 CS.reconfigure :pressurize @16:47:00.12 CS.seek 0ml @16:47:13.80 delay 10:00 @16:57:13.81 CBV.dial :waste2b @16:57:14.14 CS.reconfigure :default @16:57:14.19 CS.seek 0ml @16:57:16.17 delay 1:00 @16:58:16.20 CTV.dial :air,avoiding: :intake @16:58:17.50 Fix #2 of 2 (with RNAlater) for 10:00 @16:58:17.51 CSV.dial CSR @16:58:18.01 Pulling 2ml of CSR.RNAlater @16:58:18.02 CSR.select :RNAlater @16:58:18.13 CS.seek 2ml @16:58:24.05 delay 5 seconds @16:58:29.07 CSR.select :air @16:58:29.18 CS.reconfigure :fast @16:58:29.22 CS.seek 10ml @16:58:48.92 Pumping wcr puck from tube 4 up to 35psi... @16:58:48.93 CBV.dial SSV @16:58:50.22 CBV.dial between CSV and waste2b @16:58:51.17 CSV.dial between air and CTV @16:58:51.77 CS.seek 6.85ml @16:58:59.61 CTV.dial CSV,avoiding: :air @16:58:59.63 CSV.dial CTV @16:59:01.03 CS.reconfigure :pressurize @16:59:01.08 CS.seek 0ml @16:59:18.50 delay 10:00 @17:09:18.52 CBV.dial :waste2b @17:09:18.85 CS.reconfigure :default @17:09:18.89 CS.seek 0ml @17:09:20.22 delay 1:00 @17:10:20.25 CTV.dial :air,avoiding: :intake @17:10:21.59 Storing wcr puck in Collection Clamp to tube 3 @17:10:21.97 Elbow.reconfigure :tight @17:10:22.02 Elbow.seek 3 @17:10:23.88 Forearm.reconfigure :tight @17:10:23.93 Forearm.seek 3 + 450 counts @17:10:24.92 SE.reconfigure :pick @17:10:24.98 SE.seek :beamBreak @17:10:26.96 SE.configure :default @17:10:27.12 Elbow.reconfigure :limp @17:10:27.16 Forearm.reconfigure :loose @17:10:27.21 Forearm.seek 3 @17:10:27.48 Elbow.hold @17:10:27.55 Elbow.reconfigure :tight @17:10:27.65 SE.seek 16.11 Pucks below Top Plate @17:10:28.54 Forearm.force -20 @17:10:28.58 Forearm.seek 3 + 299 counts @17:10:28.89 Forearm.reconfigure :tight @17:10:28.93 Forearm.seek 3 + 450 counts @17:10:29.13 SE.seek 17.11 Pucks below Top Plate @17:10:29.70 Forearm.reconfigure :loose @17:10:29.74 Forearm.seek :retract @17:10:30.71 Elbow.seek CC @17:10:32.32 Elbow.reconfigure :loose @17:10:32.42 Forearm.seek CC @17:10:33.44 Hand.close @17:10:34.08 CC.seek :open @17:10:38.82 Forearm.seek :retract @17:10:39.80 Elbow.reconfigure :tight @17:10:39.85 Elbow.seek 3 @17:10:41.63 Elbow.reconfigure :limp @17:10:41.68 Forearm.seek 3 @17:10:42.68 Elbow.hold @17:10:42.74 Elbow.reconfigure :tight @17:10:42.79 Hand.open @17:10:43.57 Forearm.force -20 @17:10:43.62 Forearm.seek 3 + 311 counts @17:10:43.90 Forearm.reconfigure :tight @17:10:43.95 Forearm.seek 3 + 450 counts @17:10:44.21 SE.seek :down @17:10:45.34 Forearm.reconfigure :loose @17:10:45.38 Forearm.seek :retract @17:10:45.74 SE.stop @17:10:46.32
Starting finalPurge @17:10:46.33 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @17:10:46.51 Elbow.seek FlushPuck::Garage @17:10:48.37 Elbow.reconfigure :loose @17:10:48.46 Forearm.seek :garage @17:10:49.45 Hand.close @17:10:50.19 Forearm.seek :retract @17:10:51.14 Elbow.reconfigure :tight @17:10:51.19 Elbow.seek CC @17:10:54.24 Elbow.reconfigure :loose @17:10:54.33 Forearm.reconfigure :tight @17:10:54.38 Forearm.seek CC @17:10:55.63 CC.seek :closed @17:11:00.14 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.28 @17:11:00.22 Hand.open @17:11:00.89 Forearm.reconfigure :loose @17:11:00.93 Forearm.seek :retract @17:11:01.89 Flushing the Collection Valve::Manifold @17:11:02.01 CSV.dial CSR @17:11:02.51 Pulling 5ml of CSR.flush @17:11:02.52 CSR.select :flush @17:11:02.63 CS.seek 5ml @17:11:16.22 delay 5 seconds @17:11:21.24 CSR.select :air @17:11:21.38 CS.reconfigure :fast @17:11:21.43 CS.seek 7.501ml @17:11:28.09 CSV.dial :waste @17:11:29.35 CS.reconfigure :default @17:11:29.39 CS.seek 0ml @17:11:49.47 Flushing lower manifold's waste1 port @17:11:49.58 CSV.dial :flush @17:11:50.49 CS.reconfigure :fast @17:11:50.53 CS.seek 5ml @17:12:03.12 delay 5 seconds @17:12:08.14 CSV.dial :air @17:12:08.64 CS.seek 7ml @17:12:14.12 CSV.dial CTV @17:12:14.15
CTV.dial CSV @17:12:15.50 CBV.dial :waste1 @17:12:16.26 CS.seek 0ml @17:12:32.72 Flushing lower manifold's waste2 port @17:12:32.84 CSV.dial :flush @17:12:33.55 CS.seek 5ml @17:12:46.25 delay 5 seconds @17:12:51.27 CSV.dial :air @17:12:51.78 CS.seek 7.001ml @17:12:57.31 CSV.dial CTV @17:12:57.33
CTV.dial CSV @17:12:57.78 CBV.dial :waste2 @17:12:58.82 CS.seek 0ml @17:13:15.25 Flushing lower manifold's waste2b port @17:13:15.37 CSV.dial :flush @17:13:16.10 CS.seek 5ml @17:13:28.59 delay 5 seconds @17:13:33.61 CSV.dial :air @17:13:34.12 CS.seek 7ml @17:13:39.53 CSV.dial CTV @17:13:39.55
CTV.dial CSV @17:13:40.00 CBV.dial :waste2b @17:13:41.04 CS.seek 0ml @17:13:57.59 Disinfecting external Intake with kill @17:13:57.71 CSV.dial CSR @17:13:58.19 Pulling 8ml of CSR.kill @17:13:58.20 CSR.select :kill @17:13:58.31 CS.seek 8ml @17:14:17.94 delay 5 seconds @17:14:22.95 CSR.select :air @17:14:23.06 CS.seek 10ml @17:14:28.50 CSV.dial CBV @17:14:28.52
CBV.dial CSV @17:14:29.56 CTV.dial :intake @17:14:30.32 Intake.select :open @17:14:30.43 CS.seek 2ml @17:14:49.05 Intake.select :close @17:14:49.16 CTV.dial :air @17:14:49.92 CS.reconfigure :default @17:14:49.96 CS.seek 0ml @17:14:55.93 CSV.dial CSR @17:14:56.99 Pulling 5ml of CSR.flush @17:14:57.00 CSR.select :flush @17:14:57.11 CS.seek 5ml @17:15:10.72 delay 5 seconds @17:15:15.73 CSR.select :air @17:15:15.85 CS.seek 10ml @17:15:29.37 CSV.dial CBV @17:15:30.38 CS.seek 0ml @17:15:56.75 Garaging FlushPuck in Collection Clamp @17:15:56.81 Elbow.reconfigure :tight @17:15:56.86 Elbow.seek CC @17:15:57.16 Elbow.reconfigure :loose @17:15:57.25 Forearm.seek CC @17:15:58.20 Hand.close @17:15:58.83 CC.seek :open @17:16:03.45 Forearm.seek :retract @17:16:04.40 Elbow.reconfigure :tight @17:16:04.45 Elbow.seek :garage @17:16:07.24 Elbow.reconfigure :loose @17:16:07.33 Forearm.reconfigure :tight @17:16:07.37 Forearm.seek :garage @17:16:08.39 Hand.open @17:16:09.01 Forearm.reconfigure :loose @17:16:09.05 Forearm.seek :retract @17:16:10.01 Completed [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] begun 14:27:51 @17:16:10.06 Asleep after completing DA_WCR_031023 assays @17:16:10.08 GoodNews.email "{1 => 33,\n 2 => 33,\n 3 => 18,\n 4 => 15,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@17:07:02, 16% flow, 22.0C, 40% humidity, 14.9psia, 13.739V, 0.375A, 0.363A avg, 5.15W\nSampled 1000.0ml",Subject: "Asleep after completing DA_WCR_031023 assays" @17:16:16.21 upsync @13:12:48.60UTC15-Mar-23
ESP2 v3.41 I2C Gateway v3.20 for core bus @13:12:51.22 Configuration for short puck jake 1/11/21 by brent@mbari.org /home/esp/esp2/type/shallow/jake/configure.rb @13:12:55.22 Polling Can every 10:00 @13:12:55.47 Cleanly exited at 13:08:47UTC15-Mar-23 @13:12:55.50 Loading /home/esp/esp2/mission/DA_WCR_031523.rb mission @13:12:55.56 GoodNews.email "Starting DA_WCR_031523 w/pucks in tube 4\n{1 => 33,\n 2 => 33,\n 3 => 18,\n 4 => 15,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@13:12:55, 16% flow, 19.0C, 42% humidity, 14.8psia, 13.749V, 0.290A, 0.272A avg, 3.99W",Subject: "DA_WCR_031523 begun" @13:12:55.58
Preparing to sleep until + 00:00:05 @13:12:55.63 Asleep til + 00:00:05 @13:12:55.64 Continuing at 13:13 @13:12:55.93 Gate.power :core,:OFF @13:12:55.95 Gate.power :raw,:OFF @13:12:55.96 Shutdown @13:12:59.84 GoodNews.email "Then, in tube 4: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2/mission/DA_WCR_031523.rb:14\n{1 => 33,\n 2 => 33,\n 3 => 18,\n 4 => 15,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@13:12:55, 16% flow, 19.0C, 42% humidity, 14.8psia, 13.749V, 0.290A, 0.272A avg, 3.99W",Subject: "Asleep until 13:13" @13:13:00.53
Powering core bus @13:13:01.66 upsync @13:13:04.82 upsync @13:13:06.07
Gate.power :raw,:ON @13:13:07.05 CS.configure :default @13:13:07.14 PS.configure :default @13:13:07.24 SS.configure :default @13:13:07.33 CC.configure :default @13:13:07.42 PC.configure :default @13:13:07.53 Forearm.configure :loose @13:13:07.61 Elbow.configure :loose @13:13:07.72 SC.configure :loose @13:13:07.81 SE.configure :default @13:13:07.85 CH.coast @13:13:07.90 CH.configure :default @13:13:07.93 PH.coast @13:13:07.98 PH.configure :default @13:13:08.01 All dwarf controllers running firmware version 3.85 @13:13:08.12 Valve.reset @13:13:08.41 PSV.dial :air @13:13:08.44 Intake.select :close @13:13:08.47 Exhaust.select :close @13:13:08.50 CTV.dial :air @13:13:08.64 CSV.dial :waste1 @13:13:08.66
Forearm.reconfigure :loose @13:13:08.74 Forearm.home.seek :retract @13:13:09.12 SSV.dial :spit,avoiding: :exhaust @13:13:09.44 Hand.close @13:13:09.48
Elbow.jog 1500 @13:13:09.57 PS.reconfigure :default @13:13:09.62 PS.home.seek 0ml @13:13:09.94 CS.reconfigure :fast @13:13:09.97 SS.reconfigure :default @13:13:10.06 CS.home.seek 0ml @13:13:10.09 SS.home.seek 0ml @13:13:10.20
Elbow.reconfigure :loose @13:13:10.24 Elbow.home.seek :clear @13:13:14.71 SE.reconfigure :default @13:13:14.76 SE.home.seek :down @13:13:15.36 SC.reconfigure :tight @13:13:15.40 SC.home.seek :initial @13:13:17.84 PS.stop @13:13:43.77
SC.configure :limp @13:13:43.82 Forearm.seek :retract @13:13:43.88 Elbow.seek :hammer @13:13:44.90 Forearm.reconfigure :tight @13:13:44.95 Forearm.seek retracted @13:13:45.16 CC.reconfigure :default @13:13:45.19 PC.reconfigure :default @13:13:45.26
CC.home.seek :open @13:13:45.28 PC.home.seek :open @13:13:48.86
Phase [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] @13:13:48.87 Notice.email "At 13:13:48 began in tube 4: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2/mission/DA_WCR_031523.rb:14\n{1 => 33,\n 2 => 33,\n 3 => 18,\n 4 => 15,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@13:13:48, 16% flow, 19.1C, 41% humidity, 14.8psia, 13.710V, 0.387A, 0.369A avg, 5.31W",Subject: "Started DA_WCR_031523 phase initialPurge; da [1000, 1000] #da and wsr are run simultanelously" @13:13:48.89
Starting initialPurge @13:13:48.92 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @13:13:48.98 Hand.open @13:13:49.72 Elbow.reconfigure :tight @13:13:49.77 Elbow.seek FlushPuck::Garage @13:13:51.62 Elbow.reconfigure :loose @13:13:51.70 Forearm.reconfigure :loose @13:13:51.74 Forearm.seek :garage @13:13:52.71 Hand.close @13:13:53.47 Forearm.seek :retract @13:13:53.78 upsync @13:13:54.45
Elbow.reconfigure :tight @13:13:54.49 Elbow.seek CC @13:13:57.41 Elbow.reconfigure :loose @13:13:57.50 Forearm.reconfigure :tight @13:13:57.55 Forearm.seek CC @13:13:58.72 CC.seek :closed @13:14:03.26 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.33 @13:14:03.33 Hand.open @13:14:04.02 Forearm.reconfigure :loose @13:14:04.07 Forearm.seek :retract @13:14:05.03 Flushing the Collection Valve::Manifold @13:14:05.15 CSV.dial CSR @13:14:06.47 Pulling 5ml of CSR.flush @13:14:06.48 CSR.select :flush @13:14:06.59 CS.reconfigure :default @13:14:06.63 CS.seek 5ml @13:14:20.54 delay 5 seconds @13:14:25.56 CSR.select :air @13:14:25.70 CS.reconfigure :fast @13:14:25.75 CS.seek 7.501ml @13:14:32.48 CSV.dial :waste @13:14:33.75 CS.reconfigure :default @13:14:33.79 CS.seek 0ml @13:14:53.79 Purging external Intake @13:14:53.90 CSV.dial :flush @13:14:54.34 CS.reconfigure :fast @13:14:54.38 CS.seek 10ml @13:15:18.85 delay 5 seconds @13:15:23.87 CSV.dial CBV @13:15:23.90
CBV.dial CSV @13:15:24.77 CTV.dial :intake @13:15:25.58 Intake.select :open @13:15:25.69 CS.seek 0ml @13:15:49.26 Intake.select :close @13:15:49.35 CTV.dial :air @13:15:50.17 Flushing upper manifold @13:15:50.28 CSV.dial :flush @13:15:51.00 CS.seek 5ml @13:16:03.69 delay 5 seconds @13:16:08.71 CSV.dial :air @13:16:09.21 CS.seek 7ml @13:16:14.95 CSV.dial CBV @13:16:14.99
CBV.dial CSV @13:16:15.98 CTV.dial :air @13:16:15.99 CS.seek 0ml @13:16:32.63 delay 7 seconds @13:16:39.65 CS.seek 3ml @13:16:47.88 CSV.dial :waste @13:16:48.33 CS.seek 0ml @13:16:56.01 CSV.dial :flush @13:16:56.45 CS.seek 3ml @13:17:04.44 delay 5 seconds @13:17:09.46 CSV.dial :air @13:17:09.96 CS.seek 5ml @13:17:15.63 CSV.dial :air @13:17:15.65 CS.seek 0ml @13:17:27.85 delay 7 seconds @13:17:34.88 CS.seek 3ml @13:17:42.94 CSV.dial :waste @13:17:43.67 CS.seek 0ml @13:17:51.29 Garaging FlushPuck in Collection Clamp @13:17:51.36 Elbow.reconfigure :tight @13:17:51.40 Elbow.seek CC @13:17:51.72 Elbow.reconfigure :loose @13:17:51.81 Forearm.seek CC @13:17:52.76 Hand.close @13:17:53.34 CC.seek :open @13:17:57.96 Forearm.seek :retract @13:17:58.98 Elbow.reconfigure :tight @13:17:59.02 Elbow.seek :garage @13:18:01.96 Elbow.reconfigure :loose @13:18:02.04 Forearm.reconfigure :tight @13:18:02.09 Forearm.seek :garage @13:18:03.10 Hand.open @13:18:04.21 Forearm.reconfigure :loose @13:18:04.25 Forearm.seek :retract @13:18:05.23 da sampling at most 1000ml, wcr at most 1000ml @13:18:05.27 Loading sh1 puck from tube 4 into Collection Clamp @13:18:05.28 DAfans.email "Started da in tube 4 at 13:18:05UTC15-Mar-23\n{1 => 33,\n 2 => 33,\n 3 => 18,\n 4 => 15,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@13:13:48, 16% flow, 19.1C, 41% humidity, 14.8psia, 13.710V, 0.387A, 0.369A avg, 5.31W",Subject: "da sampling at most 1000ml, wcr at most 1000ml" @13:18:05.74
Elbow.reconfigure :tight @13:18:05.78 Elbow.seek 4 @13:18:07.70 Forearm.reconfigure :tight @13:18:07.77 Forearm.seek 4 + 450 counts @13:18:08.76 SE.reconfigure :pick @13:18:08.81 SE.seek :beamBreak @13:18:10.85 upsync @13:18:10.95
SE.configure :default @13:18:11.10 Elbow.seek 4 @13:18:11.28 Forearm.seek 4 + 300 counts @13:18:11.68 Forearm.reconfigure :loose @13:18:11.72 Hand.close @13:18:12.35 SE.seek :down @13:18:12.55
Forearm.seek :retract @13:18:13.53 Elbow.seek CC @13:18:14.21 SE.stop @13:18:15.18
Elbow.reconfigure :loose @13:18:15.27 Forearm.reconfigure :tight @13:18:15.31 Forearm.seek CC @13:18:16.55 CC.seek :closed @13:18:21.07 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.19 @13:18:21.08 Hand.open @13:18:21.77 Forearm.reconfigure :loose @13:18:21.81 Forearm.seek :retract @13:18:22.78 collectSample 1000ml w/bubble point of 24psi @13:18:22.79 Priming 7ml @13:18:22.80 Intake.select :close @13:18:22.81 Exhaust.select :close @13:18:22.82 SS.hold @13:18:23.03 Calibrating pressure and temperature sensors @13:18:23.06 CTV.dial :air @13:18:23.07 SSV.dial :spit,avoiding: :exhaust @13:18:23.09 SS.reconfigure :fast @13:18:23.13 SS.seek 5ml @13:18:25.93 SSV.dial CBV @13:18:25.96
CBV.dial SSV @13:18:27.25 delay 3.8 seconds @13:18:31.17 SSV.dial :spit @13:18:32.49 SS.seek 0ml @13:18:35.17 SSV.dial CBV @13:18:36.46 CTV.dial :intake @13:18:37.24 SSV.dial CBV @13:18:37.26
CBV.dial SSV @13:18:37.28 Allowing 40s for 7.0ml of remaining 7.0ml @13:18:37.29 Intake.select :open @13:18:39.44 Intake @14.9psia, vacuum from 9.4 to 12.4psi @13:18:39.46 SS.reconfigure :inhaling 2.20ml/s @13:18:39.50 SS.seek 7ml @13:18:46.92 Stroke completed -- waiting for pressure to equalize ... @13:18:46.93 SS.reconfigure :exhale @13:18:46.97 SS.hold @13:18:49.36 Intake.select :close @13:18:49.50 SSV.dial :exhaust @13:18:50.23 Exhaust.select :open @13:18:50.34 SS.seek 0ml @13:18:54.08 Exhaust.select :close @13:18:54.18 Sampling 1000.0ml @13:18:54.19 Intake.select :close @13:18:54.20 Exhaust.select :close @13:18:54.21 SS.reconfigure :default @13:18:54.25 SS.hold @13:18:54.43 CTV.dial :intake @13:18:54.45 SSV.dial CBV @13:18:54.46
CBV.dial SSV @13:18:55.24 Allowing 130s for 25.0ml of remaining 1000.0ml @13:18:55.25 Intake.select :open @13:18:57.40 Intake @15.4psia, vacuum from 9.9 to 12.9psi @13:18:57.41 SS.reconfigure :inhaling 2.20ml/s @13:18:57.46 SS.seek 25ml @13:19:08.16 Vacuum too high -- waiting up to 40 seconds @13:19:08.17 SS.reconfigure :exhale @13:19:08.21 SS.hold @13:19:11.49 Intake @13.6psia, vacuum from 8.1 to 11.1psi @13:19:11.50 SS.reconfigure :inhaling 1.98ml/s @13:19:11.55 SS.seek 25ml @13:19:16.98 Stroke completed -- waiting for pressure to equalize ... @13:19:16.99 SS.reconfigure :exhale @13:19:17.03 SS.hold @13:19:22.69 Intake.select :close @13:19:22.82 SSV.dial :exhaust @13:19:23.58 Exhaust.select :open @13:19:23.68 SS.seek 0ml @13:19:32.56 Exhaust.select :close @13:19:32.66 SSV.dial CBV @13:19:32.69
CBV.dial SSV @13:19:33.45 Allowing 130s for 25.0ml of remaining 975.0ml @13:19:33.46 Intake.select :open @13:19:35.68 Intake @15.6psia, vacuum from 10.0 to 13.0psi @13:19:35.71 SS.reconfigure :inhaling 1.98ml/s @13:19:35.80 SS.seek 25ml @13:19:45.45 Vacuum too high -- waiting up to 40 seconds @13:19:45.46 SS.reconfigure :exhale @13:19:45.50 SS.hold @13:19:47.03 Intake @13.6psia, vacuum from 8.1 to 11.1psi @13:19:47.04 SS.reconfigure :inhaling 1.79ml/s @13:19:47.09 SS.seek 25ml @13:19:54.43 Stroke completed -- waiting for pressure to equalize ... @13:19:54.44 SS.reconfigure :exhale @13:19:54.49 SS.hold @13:20:03.02 Intake.select :close @13:20:03.16 SSV.dial :exhaust @13:20:03.92 Exhaust.select :open @13:20:04.03 SS.seek 0ml @13:20:12.81 Exhaust.select :close @13:20:12.92 SSV.dial CBV @13:20:12.93
CBV.dial SSV @13:20:13.70 Allowing 130s for 25.0ml of remaining 950.0ml @13:20:13.71 Intake.select :open @13:20:15.86 Intake @15.6psia, vacuum from 10.0 to 13.0psi @13:20:15.88 SS.reconfigure :inhaling 1.79ml/s @13:20:15.92 SS.seek 25ml @13:20:27.19 Vacuum too high -- waiting up to 40 seconds @13:20:27.20 SS.reconfigure :exhale @13:20:27.25 SS.hold @13:20:30.43 Intake @13.9psia, vacuum from 8.4 to 11.4psi @13:20:30.45 SS.reconfigure :inhaling 1.61ml/s @13:20:30.49 SS.seek 25ml @13:20:38.00 Stroke completed -- waiting for pressure to equalize ... @13:20:38.01 SS.reconfigure :exhale @13:20:38.05 SS.hold @13:20:45.43 Intake.select :close @13:20:45.56 SSV.dial :exhaust @13:20:46.33 Exhaust.select :open @13:20:46.43 SS.seek 0ml @13:20:55.20 Exhaust.select :close @13:20:55.30 SSV.dial CBV @13:20:55.32
CBV.dial SSV @13:20:56.09 Allowing 130s for 25.0ml of remaining 925.0ml @13:20:56.10 Intake.select :open @13:20:58.25 Intake @15.6psia, vacuum from 10.0 to 13.0psi @13:20:58.27 SS.reconfigure :inhaling 1.61ml/s @13:20:58.31 SS.seek 25ml @13:21:11.47 Vacuum too high -- waiting up to 40 seconds @13:21:11.48 SS.reconfigure :exhale @13:21:11.52 SS.hold @13:21:15.86 Intake @14.2psia, vacuum from 8.7 to 11.7psi @13:21:15.87 SS.reconfigure :inhaling 1.45ml/s @13:21:15.91 SS.seek 25ml @13:21:23.00 Stroke completed -- waiting for pressure to equalize ... @13:21:23.01 SS.reconfigure :exhale @13:21:23.05 SS.hold @13:21:30.03 Intake.select :close @13:21:30.17 SSV.dial :exhaust @13:21:30.92 Exhaust.select :open @13:21:31.03 SS.seek 0ml @13:21:39.81 Exhaust.select :close @13:21:39.91 SSV.dial CBV @13:21:39.93
CBV.dial SSV @13:21:40.70 Allowing 130s for 25.0ml of remaining 900.0ml @13:21:40.71 Intake.select :open @13:21:42.87 Intake @15.5psia, vacuum from 10.0 to 13.0psi @13:21:42.88 SS.reconfigure :inhaling 1.45ml/s @13:21:42.93 SS.seek 25ml @13:21:59.03 Vacuum too high -- waiting up to 40 seconds @13:21:59.04 SS.reconfigure :exhale @13:21:59.08 SS.hold @13:22:04.05 Intake @14.0psia, vacuum from 8.5 to 11.5psi @13:22:04.06 SS.reconfigure :inhaling 1.30ml/s @13:22:04.10 SS.seek 25ml @13:22:09.06 Stroke completed -- waiting for pressure to equalize ... @13:22:09.07 SS.reconfigure :exhale @13:22:09.12 SS.hold @13:22:15.50 Intake.select :close @13:22:15.64 SSV.dial :exhaust @13:22:16.40 Exhaust.select :open @13:22:16.51 SS.seek 0ml @13:22:25.50 Exhaust.select :close @13:22:25.60 SSV.dial CBV @13:22:25.62
CBV.dial SSV @13:22:26.37 Allowing 130s for 25.0ml of remaining 875.0ml @13:22:26.38 Intake.select :open @13:22:28.53 Intake @15.4psia, vacuum from 9.9 to 12.9psi @13:22:28.55 SS.reconfigure :inhaling 1.30ml/s @13:22:28.59 SS.seek 25ml @13:22:47.00 Vacuum too high -- waiting up to 40 seconds @13:22:47.01 SS.reconfigure :exhale @13:22:47.05 SS.hold @13:22:53.60 Intake @14.5psia, vacuum from 9.0 to 12.0psi @13:22:53.61 SS.reconfigure :inhaling 1.17ml/s @13:22:53.66 SS.seek 25ml @13:22:57.51 Stroke completed -- waiting for pressure to equalize ... @13:22:57.52 SS.reconfigure :exhale @13:22:57.56 SS.hold @13:23:03.94 Intake.select :close @13:23:04.08 SSV.dial :exhaust @13:23:04.82 Exhaust.select :open @13:23:04.93 SS.seek 0ml @13:23:13.71 Exhaust.select :close @13:23:13.82 SSV.dial CBV @13:23:13.87
CBV.dial SSV @13:23:14.57 Allowing 130s for 25.0ml of remaining 850.0ml @13:23:14.58 Intake.select :open @13:23:16.74 Intake @15.4psia, vacuum from 9.9 to 12.9psi @13:23:16.76 SS.reconfigure :inhaling 1.17ml/s @13:23:16.80 SS.seek 25ml @13:23:36.37 Vacuum too high -- waiting up to 40 seconds @13:23:36.38 SS.reconfigure :exhale @13:23:36.43 SS.hold @13:23:42.93 Intake @14.6psia, vacuum from 9.1 to 12.1psi @13:23:42.94 SS.reconfigure :inhaling 1.06ml/s @13:23:42.98 SS.seek 25ml @13:23:48.45 Stroke completed -- waiting for pressure to equalize ... @13:23:48.46 SS.reconfigure :exhale @13:23:48.50 SS.hold @13:23:55.49 Intake.select :close @13:23:55.62 SSV.dial :exhaust @13:23:56.37 Exhaust.select :open @13:23:56.48 SS.seek 0ml @13:24:05.56 Exhaust.select :close @13:24:05.66 SSV.dial CBV @13:24:05.69
CBV.dial SSV @13:24:06.42 Allowing 130s for 25.0ml of remaining 825.0ml @13:24:06.43 Intake.select :open @13:24:08.58 Intake @15.4psia, vacuum from 9.9 to 12.9psi @13:24:08.59 SS.reconfigure :inhaling 1.06ml/s @13:24:08.64 SS.seek 25ml @13:24:29.63 Vacuum too high -- waiting up to 40 seconds @13:24:29.64 SS.reconfigure :exhale @13:24:29.68 SS.hold @13:24:37.18 Intake @14.8psia, vacuum from 9.3 to 12.3psi @13:24:37.19 SS.reconfigure :inhaling 0.95ml/s @13:24:37.25 SS.seek 25ml @13:24:42.74 Stroke completed -- waiting for pressure to equalize ... @13:24:42.75 SS.reconfigure :exhale @13:24:42.79 SS.hold @13:24:51.24 Intake.select :close @13:24:51.38 SSV.dial :exhaust @13:24:52.13 Exhaust.select :open @13:24:52.24 SS.seek 0ml @13:25:01.23 Exhaust.select :close @13:25:01.33 SSV.dial CBV @13:25:01.35
CBV.dial SSV @13:25:02.12 Allowing 130s for 25.0ml of remaining 800.0ml @13:25:02.13 Intake.select :open @13:25:04.28 Intake @15.4psia, vacuum from 9.9 to 12.9psi @13:25:04.30 SS.reconfigure :inhaling 0.95ml/s @13:25:04.34 SS.seek 25ml @13:25:26.87 Vacuum too high -- waiting up to 40 seconds @13:25:26.88 SS.reconfigure :exhale @13:25:26.92 SS.hold @13:25:35.45 Intake @15.0psia, vacuum from 9.5 to 12.5psi @13:25:35.47 SS.reconfigure :inhaling 0.87ml/s @13:25:35.51 SS.seek 25ml @13:25:42.30 Stroke completed -- waiting for pressure to equalize ... @13:25:42.31 SS.reconfigure :exhale @13:25:42.36 SS.hold @13:25:52.11 Intake.select :close @13:25:52.24 SSV.dial :exhaust @13:25:53.06 Exhaust.select :open @13:25:53.17 SS.seek 0ml @13:26:02.18 Exhaust.select :close @13:26:02.28 SSV.dial CBV @13:26:02.31
CBV.dial SSV @13:26:03.07 Allowing 130s for 25.0ml of remaining 775.0ml @13:26:03.08 Intake.select :open @13:26:05.23 Intake @15.4psia, vacuum from 9.9 to 12.9psi @13:26:05.25 SS.reconfigure :inhaling 0.87ml/s @13:26:05.29 SS.seek 25ml @13:26:26.18 Vacuum too high -- waiting up to 40 seconds @13:26:26.19 SS.reconfigure :exhale @13:26:26.23 SS.hold @13:26:34.21 Intake @15.1psia, vacuum from 9.6 to 12.6psi @13:26:34.22 SS.reconfigure :inhaling 0.78ml/s @13:26:34.26 SS.seek 25ml @13:26:46.02 Stroke completed -- waiting for pressure to equalize ... @13:26:46.03 SS.reconfigure :exhale @13:26:46.07 SS.hold @13:27:00.55 Intake.select :close @13:27:00.69 SSV.dial :exhaust @13:27:01.44 Exhaust.select :open @13:27:01.55 SS.seek 0ml @13:27:10.54 Exhaust.select :close @13:27:10.64 SSV.dial CBV @13:27:10.66
CBV.dial SSV @13:27:11.43 Allowing 130s for 25.0ml of remaining 750.0ml @13:27:11.44 Intake.select :open @13:27:13.59 Intake @15.4psia, vacuum from 9.9 to 12.9psi @13:27:13.61 SS.reconfigure :inhaling 0.78ml/s @13:27:13.65 SS.seek 25ml @13:27:36.47 Vacuum too high -- waiting up to 40 seconds @13:27:36.48 SS.reconfigure :exhale @13:27:36.52 SS.hold @13:27:46.29 Intake @15.1psia, vacuum from 9.6 to 12.6psi @13:27:46.31 SS.reconfigure :inhaling 0.70ml/s @13:27:46.35 SS.seek 25ml @13:27:59.38 Stroke completed -- waiting for pressure to equalize ... @13:27:59.39 SS.reconfigure :exhale @13:27:59.43 SS.hold @13:28:15.58 Intake.select :close @13:28:15.72 SSV.dial :exhaust @13:28:16.47 Exhaust.select :open @13:28:16.58 SS.seek 0ml @13:28:25.66 Exhaust.select :close @13:28:25.76 SSV.dial CBV @13:28:25.78
CBV.dial SSV @13:28:26.55 Allowing 130s for 25.0ml of remaining 725.0ml @13:28:26.56 Intake.select :open @13:28:28.71 Intake @15.5psia, vacuum from 10.0 to 13.0psi @13:28:28.73 SS.reconfigure :inhaling 0.70ml/s @13:28:28.77 SS.seek 25ml @13:28:51.81 Vacuum too high -- waiting up to 40 seconds @13:28:51.82 SS.reconfigure :exhale @13:28:51.86 SS.hold @13:29:01.61 Intake @14.8psia, vacuum from 9.3 to 12.3psi @13:29:01.62 SS.reconfigure :inhaling 0.63ml/s @13:29:01.67 SS.seek 25ml @13:29:17.89 Stroke completed -- waiting for pressure to equalize ... @13:29:17.90 SS.reconfigure :exhale @13:29:17.94 SS.hold @13:29:38.75 Intake.select :close @13:29:38.89 SSV.dial :exhaust @13:29:39.65 Exhaust.select :open @13:29:39.76 SS.seek 0ml @13:29:48.80 Exhaust.select :close @13:29:48.90 SSV.dial CBV @13:29:48.92
CBV.dial SSV @13:29:49.69 Allowing 130s for 25.0ml of remaining 700.0ml @13:29:49.70 Intake.select :open @13:29:51.85 Intake @15.2psia, vacuum from 9.7 to 12.7psi @13:29:51.87 SS.reconfigure :inhaling 0.63ml/s @13:29:51.91 SS.seek 25ml @13:30:15.77 Vacuum too high -- waiting up to 40 seconds @13:30:15.78 SS.reconfigure :exhale @13:30:15.83 SS.hold @13:30:28.44 Intake @14.9psia, vacuum from 9.4 to 12.4psi @13:30:28.46 SS.reconfigure :inhaling 0.57ml/s @13:30:28.51 SS.seek 25ml @13:30:48.17 Stroke completed -- waiting for pressure to equalize ... @13:30:48.18 SS.reconfigure :exhale @13:30:48.22 SS.hold @13:31:14.60 Intake.select :close @13:31:14.73 SSV.dial :exhaust @13:31:15.49 Exhaust.select :open @13:31:15.60 SS.seek 0ml @13:31:24.45 Exhaust.select :close @13:31:24.56 SSV.dial CBV @13:31:24.61
CBV.dial SSV @13:31:25.33 Allowing 130s for 25.0ml of remaining 675.0ml @13:31:25.34 Intake.select :open @13:31:27.78 Intake @15.3psia, vacuum from 9.8 to 12.8psi @13:31:27.79 SS.reconfigure :inhaling 0.57ml/s @13:31:27.84 SS.seek 25ml @13:31:50.99 Vacuum too high -- waiting up to 40 seconds @13:31:51.00 SS.reconfigure :exhale @13:31:51.04 SS.hold @13:32:03.66 Intake @14.9psia, vacuum from 9.4 to 12.4psi @13:32:03.68 SS.reconfigure :inhaling 0.51ml/s @13:32:03.72 SS.seek 25ml @13:32:24.12 Vacuum too high -- waiting up to 40 seconds @13:32:24.13 SS.reconfigure :exhale @13:32:24.17 SS.hold @13:32:40.53 Intake @15.0psia, vacuum from 9.5 to 12.5psi @13:32:40.55 SS.reconfigure :inhaling 0.47ml/s @13:32:40.59 SS.seek 25ml @13:32:47.65 Stroke completed -- waiting for pressure to equalize ... @13:32:47.66 SS.reconfigure :exhale @13:32:47.70 SS.hold @13:33:08.06 Intake.select :close @13:33:08.19 SSV.dial :exhaust @13:33:08.96 Exhaust.select :open @13:33:09.06 SS.seek 0ml @13:33:18.23 Exhaust.select :close @13:33:18.34 SSV.dial CBV @13:33:18.39
CBV.dial SSV @13:33:19.11 Allowing 130s for 25.0ml of remaining 650.0ml @13:33:19.12 Intake.select :open @13:33:21.27 Intake @15.2psia, vacuum from 9.7 to 12.7psi @13:33:21.29 SS.reconfigure :inhaling 0.47ml/s @13:33:21.33 SS.seek 25ml @13:33:50.43 Vacuum too high -- waiting up to 40 seconds @13:33:50.44 SS.reconfigure :exhale @13:33:50.48 SS.hold @13:34:08.67 Intake @15.1psia, vacuum from 9.6 to 12.6psi @13:34:08.69 SS.reconfigure :inhaling 0.43ml/s @13:34:08.73 SS.seek 25ml @13:34:37.02 Vacuum too high -- waiting up to 40 seconds @13:34:37.03 SS.reconfigure :exhale @13:34:37.07 SS.hold @13:35:01.41 Intake @15.3psia, vacuum from 9.8 to 12.8psi @13:35:01.43 SS.reconfigure :inhaling 0.38ml/s @13:35:01.47 SS.seek 25ml @13:35:02.56 Stroke completed -- waiting for pressure to equalize ... @13:35:02.57 SS.reconfigure :exhale @13:35:02.61 SS.hold @13:35:20.83 Intake.select :close @13:35:20.97 SSV.dial :exhaust @13:35:21.73 Exhaust.select :open @13:35:21.84 SS.seek 0ml @13:35:30.96 Exhaust.select :close @13:35:31.07 SSV.dial CBV @13:35:31.09
CBV.dial SSV @13:35:31.86 Allowing 130s for 25.0ml of remaining 625.0ml @13:35:31.87 Intake.select :open @13:35:34.02 Intake @15.2psia, vacuum from 9.7 to 12.7psi @13:35:34.04 SS.reconfigure :inhaling 0.38ml/s @13:35:34.08 SS.seek 25ml @13:36:09.45 Vacuum too high -- waiting up to 40 seconds @13:36:09.46 SS.reconfigure :exhale @13:36:09.51 SS.hold @13:36:31.84 Intake @15.5psia, vacuum from 10.0 to 13.0psi @13:36:31.86 SS.reconfigure :inhaling 0.34ml/s @13:36:31.90 SS.seek 25ml @13:37:10.70 Stroke completed -- waiting for pressure to equalize ... @13:37:10.71 SS.reconfigure :exhale @13:37:10.75 SS.hold @13:37:41.88 SS.reconfigure :default @13:37:41.91 SS.hold @13:37:42.02 Intake.select :close @13:37:42.16 ShallowSampler::Clogged in MAIN -- Filtering too Slowly after 400.0ml @13:37:42.17 SSV.dial :exhaust @13:37:42.93 Exhaust.select :open @13:37:43.04 SS.reconfigure :exhale @13:37:43.08 SS.seek 0ml @13:37:52.10 Exhaust.select :close @13:37:52.20 Sampled 400.0ml @13:37:52.22 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @13:37:52.23 Intake.select :close @13:37:52.28 CSV.dial :waste @13:37:52.34 CS.seek 0ml @13:37:52.41 SSV.dial CBV,avoiding: :exhaust @13:37:52.43
CBV.dial SSV @13:37:52.49 CSV.dial :air @13:37:53.23 CS.seek 10ml @13:37:54.31
CTV.dial :air @13:37:55.10 delay 15 seconds @13:38:10.11 SSV.dial :spit,avoiding: :exhaust @13:38:11.44 CTV.dial CSV,avoiding: :intake @13:38:11.48
SS.seek 0ml @13:38:11.57 SSV.dial CBV,avoiding: :exhaust @13:38:17.91 CBV.dial between SSV and waste2 @13:38:18.31 CTV.dial CSV,avoiding: :air @13:38:18.33
CSV.dial CTV @13:38:18.78 CS.reconfigure :pressurize @13:38:18.82 CS.seek 0ml @13:38:37.36 BubblePoint: Intake @39.2psia, Outlet @15.1psia @13:38:37.37 CBV.dial SSV @13:38:37.71 SS.reconfigure :default @13:38:37.75 SS.seek 10ml @13:38:43.85 delay 15 seconds @13:38:58.96 Evacuated: Intake @16.9psia, Outlet @9.7psia @13:38:58.98 SSV.dial :spit,avoiding: :exhaust @13:39:00.31 SS.reconfigure :exhale @13:39:00.35 SS.seek 0ml @13:39:05.74 SS.reconfigure :fast @13:39:05.78 SS.seek 5ml @13:39:08.62 SSV.dial CBV,avoiding: :exhaust @13:39:08.64
CBV.dial SSV @13:39:09.98 CTV.dial :air,avoiding: :intake @13:39:11.33 CSV.dial CTV @13:39:11.36
SS.seek 10ml @13:39:12.37 CSV.dial :air @13:39:12.82 CS.reconfigure :fast @13:39:12.86 CS.seek 0ml @13:39:13.84
SSV.dial :spit,avoiding: :exhaust @13:39:15.15 SS.reconfigure :exhale @13:39:15.20 SS.seek 0ml @13:39:20.13 SS.reconfigure :fast @13:39:20.17 SS.seek 5ml @13:39:20.48 CS.stop @13:39:23.02 SSV.dial CBV,avoiding: :exhaust @13:39:23.03
CBV.dial SSV @13:39:25.38 SSV.dial :spit,avoiding: :exhaust @13:39:26.70 SS.reconfigure :default @13:39:26.74 SS.seek 0ml @13:39:30.00 SS.stop @13:39:30.04 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @13:39:30.05 Intake.select :close @13:39:30.12 CSV.dial :waste @13:39:30.23 SSV.dial CBV,avoiding: :exhaust @13:39:30.25
CBV.dial SSV @13:39:30.90 CS.seek 0ml @13:39:30.96 CSV.dial :air @13:39:31.59
CTV.dial :air @13:39:31.60 delay 15 seconds @13:39:31.71 CS.seek 10ml @13:39:46.62
SSV.dial :spit,avoiding: :exhaust @13:39:47.94 CTV.dial CSV,avoiding: :intake @13:39:47.96
SS.reconfigure :exhale @13:39:48.06 SS.seek 0ml @13:39:48.46 SSV.dial CBV,avoiding: :exhaust @13:39:56.10 CBV.dial between SSV and waste2 @13:39:56.50 CTV.dial CSV,avoiding: :air @13:39:56.52
CSV.dial CTV @13:39:56.96 CS.reconfigure :pressurize @13:39:57.00 CS.seek 0ml @13:40:18.30 BubblePoint: Intake @38.2psia, Outlet @14.1psia @13:40:18.31 CBV.dial SSV @13:40:18.64 SS.reconfigure :default @13:40:18.69 SS.seek 10ml @13:40:24.19 delay 15 seconds @13:40:39.31 Evacuated: Intake @18.2psia, Outlet @11.6psia @13:40:39.32 SSV.dial :spit,avoiding: :exhaust @13:40:40.64 SS.reconfigure :exhale @13:40:40.68 SS.seek 0ml @13:40:45.87 SS.reconfigure :fast @13:40:45.91 SS.seek 5ml @13:40:48.89 SSV.dial CBV,avoiding: :exhaust @13:40:48.90
CBV.dial SSV @13:40:50.24 CTV.dial :air,avoiding: :intake @13:40:51.59 CSV.dial CTV @13:40:51.63
SS.seek 10ml @13:40:52.62 CSV.dial :air @13:40:53.08 CS.reconfigure :fast @13:40:53.13 CS.seek 0ml @13:40:54.23
SSV.dial :spit,avoiding: :exhaust @13:40:55.54 SS.reconfigure :exhale @13:40:55.59 SS.seek 0ml @13:40:58.48 CS.stop @13:41:00.58
SS.reconfigure :fast @13:41:00.62 SS.seek 5ml @13:41:03.45 SSV.dial CBV,avoiding: :exhaust @13:41:03.47
CBV.dial SSV @13:41:05.79 SSV.dial :spit,avoiding: :exhaust @13:41:07.10 SS.reconfigure :default @13:41:07.15 SS.seek 0ml @13:41:10.38 SS.stop @13:41:10.41 Lysing: Adding 2.0ml AqMeOH @13:41:10.43 CBV.dial between CSV and waste1,avoiding: :DBV @13:41:11.92 CTV.dial CSV @13:41:13.25 CSV.dial CSR @13:41:14.03 Pulling 1ml of CSR.AqMeOH @13:41:14.04 CSR.select :AqMeOH @13:41:14.15 CS.reconfigure :default @13:41:14.19 CS.seek 1ml @13:41:17.80 delay 5 seconds @13:41:22.81 CSR.select :air @13:41:22.96 CS.seek 6ml @13:41:36.64 delay 2 seconds @13:41:38.65 CSV.dial :waste2 @13:41:39.53 CS.reconfigure :fast @13:41:39.58 CS.seek 0ml @13:41:53.95 CSV.dial CSR @13:41:54.46 Pulling 2ml of CSR.AqMeOH @13:41:54.47 CSR.select :AqMeOH @13:41:54.58 CS.reconfigure :default @13:41:54.62 CS.seek 2ml @13:42:00.64 delay 5 seconds @13:42:05.66 CSR.select :air @13:42:05.81 CS.seek 5.001ml @13:42:14.26 delay 2 seconds @13:42:16.28 CSV.dial CTV @13:42:16.71 CS.seek 1ml @13:42:28.02 Before lysing Collection Puck Heater at 18.6C/38.0psia @13:42:28.04 Heating Puck.from 4, :sh1 in Collection Clamp to 70C for 10:00 @13:42:28.06 CH.seek 70C,8:00 @13:44:27.46 CH.hold 10:00,70C @13:44:27.64 Begun lysing Collection Puck Heater at 68C/45.7psia @13:54:27.65 Ended lysing Collection Puck Heater at 70C/44.2psia @13:54:27.67 CS.seek 0ml @13:54:31.25 CTV.dial between spare and air,avoiding: :intake @13:54:32.30 CSV.dial :air @13:54:32.75 CS.seek 5ml @13:54:46.34 CSV.dial CTV @13:54:46.80 delay 2 seconds @13:54:48.81 CSV.dial :air @13:54:49.27 delay 2 seconds @13:54:51.28 CSV.dial CTV @13:54:51.73 delay 2 seconds @13:54:53.75 CSV.dial :air @13:54:54.20 delay 2 seconds @13:54:56.22 CS.seek 0ml @13:55:09.96 Puck @71.3psia after final lysis pressurization @13:55:09.97 Recovering Lysate into Collection syringe @13:55:09.98 CSV.dial CBV @13:55:10.98 CBV.dial CSV @13:55:11.34 CS.seek 5ml @13:55:25.15 delay 5 seconds @13:55:30.17 CTV.dial :air @13:55:30.70 CS.seek 6.999ml @13:55:36.59 delay 5 seconds @13:55:41.61 CSV.dial :air2 @13:55:42.05 CS.seek 10ml @13:55:50.53 CSV.dial between air and CTV,avoiding: CBV @13:55:51.68 Lysing Complete @13:55:51.74 Storing sh1 puck in Collection Clamp to tube 3 @13:55:51.90 SC.reconfigure :tight @13:55:52.43 SC.seek 3 @13:55:56.02 SC.configure :limp @13:55:56.21 Elbow.reconfigure :tight @13:55:56.26 Elbow.seek 3 @13:55:58.03 Forearm.reconfigure :tight @13:55:58.07 Forearm.seek 3 + 450 counts @13:55:59.08 SE.reconfigure :pick @13:55:59.13 SE.seek :beamBreak @13:56:01.07 SE.configure :default @13:56:01.22 Elbow.reconfigure :limp @13:56:01.26 Forearm.reconfigure :loose @13:56:01.30 Forearm.seek 3 @13:56:01.57 Elbow.hold @13:56:01.63 Elbow.reconfigure :tight @13:56:01.72 SE.seek 17.12 Pucks below Top Plate @13:56:02.17 Forearm.force -20 @13:56:02.20 Forearm.seek 3 + 284 counts @13:56:02.48 Forearm.reconfigure :tight @13:56:02.53 Forearm.seek 3 + 450 counts @13:56:02.76 SE.seek 18.12 Pucks below Top Plate @13:56:03.40 Forearm.reconfigure :loose @13:56:03.45 Forearm.seek :retract @13:56:04.40 Elbow.seek CC @13:56:06.05 Elbow.reconfigure :loose @13:56:06.14 Forearm.seek CC @13:56:07.13 Hand.close @13:56:07.68 CC.seek :open @13:56:12.40 Forearm.seek :retract @13:56:13.39 Elbow.reconfigure :tight @13:56:13.43 Elbow.seek 3 @13:56:15.19 Elbow.reconfigure :limp @13:56:15.24 Forearm.seek 3 @13:56:16.20 Elbow.hold @13:56:16.26 Elbow.reconfigure :tight @13:56:16.30 Hand.open @13:56:17.04 Forearm.force -20 @13:56:17.07 Forearm.seek 3 + 316 counts @13:56:17.37 Forearm.reconfigure :tight @13:56:17.41 Forearm.seek 3 + 450 counts @13:56:17.68 SE.seek :down @13:56:18.81
Forearm.reconfigure :loose @13:56:18.85 Forearm.seek :retract @13:56:19.17 SE.stop @13:56:19.78
Tranferring DA @13:56:19.79 Beginning samplePassC @13:56:19.80 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @13:56:19.97 Elbow.seek FlushPuck::Garage @13:56:21.75 Elbow.reconfigure :loose @13:56:21.84 Forearm.seek :garage @13:56:22.84 Hand.close @13:56:23.54 Forearm.seek :retract @13:56:24.54 Elbow.reconfigure :tight @13:56:24.59 Elbow.seek CC @13:56:27.47 Elbow.reconfigure :loose @13:56:27.56 Forearm.reconfigure :tight @13:56:27.60 Forearm.seek CC @13:56:28.77 CC.seek :closed @13:56:33.31 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.36 @13:56:33.38 Hand.open @13:56:33.99 Forearm.reconfigure :loose @13:56:34.03 Forearm.seek :retract @13:56:35.44 PSV.dial PSR @13:56:36.83 Pulling 1.5ml of PSR.flush PSR.select :flush @13:56:36.95 PS.seek 1.5ml @13:56:53.07 delay 5 seconds @13:56:58.09 PSV.dial :waste1 @13:56:58.56 PS.seek 0ml @13:57:14.66 PSV.dial PSR @13:57:15.13 PSR.select :air @13:57:15.24 PS.seek 2.5ml @13:57:41.61 PRV.dial CBV,avoiding: :ab2 @13:57:41.62
CBV.dial PRV @13:57:43.32 PSV.dial :mixloop @13:57:44.48 CTV.dial :air,avoiding: :intake @13:57:44.49 PTV.dial :PRVmixing @13:57:45.81 PS.seek 0ml @13:58:12.19 PSV.dial :air @13:58:12.65 PS.seek 2.5ml @13:58:39.06 PSV.dial :mixloop @13:58:39.53 PS.seek 0ml @13:59:05.81 PSV.dial :air @13:59:06.28 PS.seek 2.5ml @13:59:32.59 PSV.dial :mixloop @13:59:33.06 PS.seek 0ml @13:59:59.45 Garaging FlushPuck in Collection Clamp @13:59:59.52 Elbow.reconfigure :tight @13:59:59.56 Elbow.seek CC @13:59:59.93 Elbow.reconfigure :loose @14:00:00.02 Forearm.seek CC @14:00:00.97 Hand.close @14:00:01.56 CC.seek :open @14:00:06.20 Forearm.seek :retract @14:00:07.16 Elbow.reconfigure :tight @14:00:07.21 Elbow.seek :garage @14:00:10.07 Elbow.reconfigure :loose @14:00:10.16 Forearm.reconfigure :tight @14:00:10.20 Forearm.seek :garage @14:00:11.13 Hand.open @14:00:11.86 Forearm.reconfigure :loose @14:00:11.90 Forearm.seek :retract @14:00:12.89 PSV.dial :mixloop @14:00:12.91 PTV.dial :PRVmixing @14:00:12.92 PRV.dial CBV @14:00:12.94
CBV.dial PRV @14:00:12.96 CTV.dial :air,avoiding: :intake @14:00:12.98 Loading lyfil puck from tube 4 into Collection Clamp @14:00:13.19 SC.reconfigure :tight @14:00:13.23 SC.seek 4 @14:00:16.84 SC.configure :limp @14:00:17.03 Elbow.reconfigure :tight @14:00:17.07 Elbow.seek 4 @14:00:18.97 Forearm.reconfigure :tight @14:00:19.02 Forearm.seek 4 + 450 counts @14:00:20.02 SE.reconfigure :pick @14:00:20.07 SE.seek :beamBreak @14:00:22.26 SE.configure :default @14:00:22.41 Elbow.seek 4 @14:00:22.58 Forearm.seek 4 + 300 counts @14:00:22.85 Forearm.reconfigure :loose @14:00:22.89 Hand.close @14:00:23.57 SE.seek :down @14:00:23.75
Forearm.seek :retract @14:00:24.70 Elbow.seek CC @14:00:25.44 SE.stop @14:00:26.33
Elbow.reconfigure :loose @14:00:26.42 Forearm.reconfigure :tight @14:00:26.46 Forearm.seek CC @14:00:27.75 CC.seek :closed @14:00:32.26 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.09 @14:00:32.27 Hand.open @14:00:32.99 Forearm.reconfigure :loose @14:00:33.03 Forearm.seek :retract @14:00:33.96 CTV.dial CSV,avoiding: :intake @14:00:35.34 CSV.dial CTV @14:00:35.64 PS.seek 2.5ml @14:01:02.10 CS.seek 4.5ml @14:01:16.99 delay 8 seconds @14:01:25.01 PSV.dial :air @14:01:25.47 delay 2 seconds @14:01:27.49 PSV.dial :waste2 @14:01:28.23 PS.seek 0ml @14:01:54.53 CBV.dial between PRV and waste1 @14:01:54.85 CSV.dial :air @14:01:55.31 CS.seek 10ml @14:02:10.15 CSV.dial CTV @14:02:10.61 CS.seek 0ml @14:02:37.21 CBV.dial :waste1 @14:02:37.51 delay 5 seconds @14:02:42.53 CTV.dial :air,avoiding: :intake @14:02:43.94 Storing lyfil puck in Collection Clamp to tube 3 @14:02:44.10 SC.reconfigure :tight @14:02:44.14 SC.seek 3 @14:02:47.74 SC.configure :limp @14:02:47.92 Elbow.reconfigure :tight @14:02:47.97 Elbow.seek 3 @14:02:49.74 Forearm.reconfigure :tight @14:02:49.78 Forearm.seek 3 + 450 counts @14:02:50.81 SE.reconfigure :pick @14:02:50.86 SE.seek :beamBreak @14:02:52.88 SE.configure :default @14:02:53.02 Elbow.reconfigure :limp @14:02:53.06 Forearm.reconfigure :loose @14:02:53.10 Forearm.seek 3 @14:02:53.27 Elbow.hold @14:02:53.33 Elbow.reconfigure :tight @14:02:53.42 SE.seek 18.12 Pucks below Top Plate @14:02:53.98 Forearm.force -20 @14:02:54.01 Forearm.seek 3 + 318 counts @14:02:54.29 Forearm.reconfigure :tight @14:02:54.33 Forearm.seek 3 + 450 counts @14:02:54.51 SE.seek 19.12 Pucks below Top Plate @14:02:55.03 Forearm.reconfigure :loose @14:02:55.08 Forearm.seek :retract @14:02:56.01 Elbow.seek CC @14:02:57.68 Elbow.reconfigure :loose @14:02:57.77 Forearm.seek CC @14:02:58.74 Hand.close @14:02:59.30 CC.seek :open @14:03:04.01 Forearm.seek :retract @14:03:04.97 Elbow.reconfigure :tight @14:03:05.02 Elbow.seek 3 @14:03:06.77 Elbow.reconfigure :limp @14:03:06.81 Forearm.seek 3 @14:03:07.75 Elbow.hold @14:03:07.81 Elbow.reconfigure :tight @14:03:07.85 Hand.open @14:03:08.54 Forearm.force -20 @14:03:08.57 Forearm.seek 3 + 318 counts @14:03:08.85 Forearm.reconfigure :tight @14:03:08.90 Forearm.seek 3 + 450 counts @14:03:09.15 SE.seek :down @14:03:10.68
Forearm.reconfigure :loose @14:03:10.81 SE.stop @14:03:10.82
Forearm.seek :retract @14:03:11.78 PSV.dial :air @14:03:12.54 PS.seek 2.5ml @14:03:38.90 delay 1 second @14:03:39.92 PSV.dial :mixloop @14:03:40.47 delay 1 second @14:03:41.48 PSV.dial :air @14:03:42.05 delay 1 second @14:03:43.06 PSV.dial :mixloop @14:03:43.50 delay 1 second @14:03:44.51 PSV.dial :air @14:03:44.98 delay 1 second @14:03:46.00 PS.seek 0ml @14:04:12.41 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @14:04:12.58 Elbow.seek FlushPuck::Garage @14:04:14.37 Elbow.reconfigure :loose @14:04:14.46 Forearm.seek :garage @14:04:15.47 Hand.close @14:04:16.16 Forearm.seek :retract @14:04:17.15 Elbow.reconfigure :tight @14:04:17.19 Elbow.seek PC @14:04:18.42 Elbow.reconfigure :loose @14:04:18.50 Forearm.reconfigure :tight @14:04:18.55 Forearm.seek PC @14:04:19.75 PC.seek :closed @14:04:24.38 Processing Clamp onSpring/offSpring for 16/26 samples, on/off torque 1.55 @14:04:24.46 Hand.open @14:04:25.11 Forearm.reconfigure :loose @14:04:25.15 Forearm.seek :retract @14:04:26.11 PRV.dial :air @14:04:26.56 PSV.dial :mixloop @14:04:27.09 PS.seek 0.3ml @14:04:31.06 PSV.dial PSR @14:04:32.14 Pulling 0.4ml of PSR.air @14:04:32.15 PSR.select :air @14:04:32.18 PS.seek 0.7ml @14:04:37.12 delay 3 seconds @14:04:40.14 PSR.select :air @14:04:40.16 PS.seek 2.5ml @14:04:59.37 delay 3 seconds @14:05:02.39 PSV.dial :mixloop @14:05:03.42 PTV.dial :mixing @14:05:04.17 PBV.dial :waste2 @14:05:05.11 PS.seek 0ml @14:05:31.47 Garaging FlushPuck in Processing Clamp @14:05:31.53 Elbow.reconfigure :tight @14:05:31.58 Elbow.seek PC @14:05:31.68 Elbow.reconfigure :loose @14:05:31.77 Forearm.seek PC @14:05:32.72 Hand.close @14:05:33.30 PC.seek :open @14:05:38.04 Forearm.seek :retract @14:05:38.99 Elbow.reconfigure :tight @14:05:39.03 Elbow.seek :garage @14:05:40.37 Elbow.reconfigure :loose @14:05:40.45 Forearm.reconfigure :tight @14:05:40.50 Forearm.seek :garage @14:05:41.55 Hand.open @14:05:42.23 Forearm.reconfigure :loose @14:05:42.27 Forearm.seek :retract @14:05:43.26 Loading da puck from tube 4 into Processing Clamp @14:05:43.47 SC.reconfigure :tight @14:05:43.52 SC.seek 4 @14:05:47.06 SC.configure :limp @14:05:47.24 Elbow.reconfigure :tight @14:05:47.29 Elbow.seek 4 @14:05:49.25 Forearm.reconfigure :tight @14:05:49.30 Forearm.seek 4 + 450 counts @14:05:50.37 SE.reconfigure :pick @14:05:50.42 SE.seek :beamBreak @14:05:53.18 SE.configure :default @14:05:53.33 Elbow.seek 4 @14:05:53.62 Forearm.seek 4 + 300 counts @14:05:53.77 Forearm.reconfigure :loose @14:05:53.81 Hand.close @14:05:54.44 SE.seek :down @14:05:54.63
Forearm.seek :retract @14:05:55.57 Elbow.seek PC @14:05:56.45 SE.stop @14:05:58.07
Elbow.reconfigure :loose @14:05:58.16 Forearm.reconfigure :tight @14:05:58.20 Forearm.seek PC @14:05:59.41 PC.seek :closed @14:06:04.03 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.59 @14:06:04.04 Hand.open @14:06:04.74 Forearm.reconfigure :loose @14:06:04.78 Forearm.seek :retract @14:06:05.71 Heating Puck.from 4, :da in Processing Clamp to 28C for 2:00:00 @14:06:05.73 PH.seek 28C,5:00 @14:06:39.30 PH.hold 2:00:00,28C @14:06:39.34 delay 1:30 @14:08:09.38 PSR.select :air @14:08:09.40 PSV.dial PSR @14:08:10.48 Pulling 0.5ml of PSR.wash @14:08:10.49 PSR.select :wash @14:08:10.61 PS.seek 0.5ml @14:08:16.59 delay 5 seconds @14:08:21.61 PSR.select :air @14:08:21.72 PS.seek 2ml @14:08:37.90 PTV.dial :puck @14:08:38.63 PSV.dial PTV @14:08:39.40 PBV.dial :waste1 @14:08:40.79 PS.seek 1.5ml @14:08:46.77 delay 3 seconds @14:08:49.79 PBV.dial between PSV and waste1 @14:08:50.16 PS.seek 0ml @14:09:06.27 delay 5:00 @14:14:06.30 recoverPSRreagent08 @14:14:06.31 PTV.dial :puck @14:14:06.32 PBV.dial :waste1 @14:14:06.69 delay 30 seconds @14:14:36.71 PBV.dial between PSV and waste1 @14:14:37.08 PSR.select :air @14:14:37.10 PSV.dial PSR @14:14:37.87 PS.reconfigure :fast @14:14:37.91 PS.seek 2.5ml @14:15:01.64 PSV.dial PTV @14:15:02.39 delay 5 seconds @14:15:07.41 PSV.dial PSR @14:15:08.16 delay 5 seconds @14:15:13.17 PSV.dial PTV @14:15:14.05 delay 5 seconds @14:15:19.07 PSV.dial :air @14:15:19.85 PS.seek 0ml @14:15:43.17 PTV.dial :PRVmixing,via: PRV @14:15:44.48 PSV.dial :mixloop @14:15:44.94 PRV.dial :ab1 @14:15:45.66 PS.reconfigure :slow @14:15:45.70 PS.seek 0.15ml @14:15:49.48 delay 2 seconds @14:15:51.50 CBV.dial PRV @14:15:51.52
PRV.dial CBV @14:15:52.10 PS.seek 0.35ml @14:15:56.70 delay 2 seconds @14:15:58.72 CBV.dial between PRV and PSV @14:15:59.03 PRV.dial :air @14:15:59.48 PS.seek 0.9ml @14:16:11.64 PRV.dial :waste2 @14:16:12.37 PS.seek 0.5ml @14:16:21.01 addPMLReagent08 @14:16:21.02 PSV.dial :air @14:16:21.49 PS.reconfigure :default @14:16:21.53 PS.seek 2ml @14:16:37.76 PSV.dial :PML @14:16:38.22 PTV.dial :mixing @14:16:38.95 PBV.dial :waste1 @14:16:39.37 PS.seek 1.85ml @14:16:41.70 PBV.dial between PSV and waste1 @14:16:42.07 PS.seek 0ml @14:17:01.79 delay 2 seconds @14:17:03.81 Beginning samplePassC @14:17:03.82 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:17:03.99 Elbow.reconfigure :tight @14:17:04.03 Elbow.seek FlushPuck::Garage @14:17:05.37 Elbow.reconfigure :loose @14:17:05.46 Forearm.seek :garage @14:17:06.44 Hand.close @14:17:07.17 Forearm.seek :retract @14:17:08.15 Elbow.reconfigure :tight @14:17:08.19 Elbow.seek CC @14:17:11.08 Elbow.reconfigure :loose @14:17:11.17 Forearm.reconfigure :tight @14:17:11.21 Forearm.seek CC @14:17:12.45 CC.seek :closed @14:17:16.96 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.42 @14:17:17.04 Hand.open @14:17:17.72 Forearm.reconfigure :loose @14:17:17.76 Forearm.seek :retract @14:17:18.72 PSV.dial PSR @14:17:19.83 Pulling 1.5ml of PSR.flush @14:17:19.84 PSR.select :flush @14:17:19.96 PS.seek 1.5ml @14:17:36.05 delay 5 seconds @14:17:41.06 PSV.dial :waste1 @14:17:41.52 PS.seek 0ml @14:17:57.64 PSV.dial PSR @14:17:58.09 PSR.select :air @14:17:58.20 PS.seek 2.5ml @14:18:24.77 PRV.dial CBV,avoiding: :ab2 @14:18:24.79
CBV.dial PRV @14:18:26.40 PSV.dial :mixloop @14:18:27.44 CTV.dial :air,avoiding: :intake @14:18:27.45 PTV.dial :PRVmixing @14:18:28.21 PS.seek 0ml @14:18:54.48 PSV.dial :air @14:18:54.94 PS.seek 2.5ml @14:19:21.32 PSV.dial :mixloop @14:19:21.78 PS.seek 0ml @14:19:48.16 PSV.dial :air @14:19:48.63 PS.seek 2.5ml @14:20:15.04 PSV.dial :mixloop @14:20:15.50 PS.seek 0ml @14:20:41.88 Garaging FlushPuck in Collection Clamp @14:20:41.95 Elbow.reconfigure :tight @14:20:41.99 Elbow.seek CC @14:20:42.34 Elbow.reconfigure :loose @14:20:42.43 Forearm.seek CC @14:20:43.39 Hand.close @14:20:44.04 CC.seek :open @14:20:48.65 Forearm.seek :retract @14:20:49.65 Elbow.reconfigure :tight @14:20:49.69 Elbow.seek :garage @14:20:52.53 Elbow.reconfigure :loose @14:20:52.61 Forearm.reconfigure :tight @14:20:52.66 Forearm.seek :garage @14:20:54.15 Hand.open @14:20:54.84 Forearm.reconfigure :loose @14:20:54.89 Forearm.seek :retract @14:20:55.87 delayUntil "14:25:03.80" @14:20:55.89 Whole Cell Archive sampling 1000ml @14:20:55.91 Loading wcr puck from tube 4 into Collection Clamp @14:20:56.35 Elbow.reconfigure :tight @14:20:56.40 Elbow.seek 4 @14:20:58.30 Forearm.reconfigure :tight @14:20:58.34 Forearm.seek 4 + 450 counts @14:20:59.49 SE.reconfigure :pick @14:20:59.54 SE.seek :beamBreak @14:21:01.84 SE.configure :default @14:21:02.00 Elbow.seek 4 @14:21:02.17 Forearm.seek 4 + 300 counts @14:21:02.30 Forearm.reconfigure :loose @14:21:02.34 Hand.close @14:21:02.93 SE.seek :down @14:21:03.12 Forearm.seek :retract @14:21:04.07 Elbow.seek CC @14:21:04.97 SE.stop @14:21:05.70 Elbow.reconfigure :loose @14:21:05.80 Forearm.reconfigure :tight @14:21:05.84 Forearm.seek CC @14:21:07.08 CC.seek :closed @14:21:11.60 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.23 @14:21:11.61 Hand.open @14:21:12.22 Forearm.reconfigure :loose @14:21:12.27 Forearm.seek :retract @14:21:13.24 collectSample 1000ml w/bubble point of 35psi @14:21:13.25 Priming 7ml @14:21:13.26 Intake.select :close @14:21:13.27 Exhaust.select :close @14:21:13.29 SS.hold @14:21:13.48 Calibrating pressure sensors @14:21:13.49 CTV.dial :air @14:21:13.50 SSV.dial :spit,avoiding: :exhaust @14:21:13.52 SS.reconfigure :fast @14:21:13.57 SS.seek 5ml @14:21:16.23 SSV.dial CBV @14:21:16.28 CBV.dial SSV @14:21:17.57 delay 3.8 seconds @14:21:21.49 SSV.dial :spit @14:21:22.81 SS.seek 0ml @14:21:25.55 SSV.dial CBV @14:21:26.88 CTV.dial :intake @14:21:27.65 SSV.dial CBV @14:21:27.67 CBV.dial SSV @14:21:27.69 Allowing 40s for 7.0ml of remaining 7.0ml @14:21:27.70 Intake.select :open @14:21:29.85 Intake @15.0psia, vacuum from 9.5 to 12.5psi @14:21:29.87 SS.reconfigure :inhaling 2.20ml/s @14:21:29.91 SS.seek 7ml @14:21:36.74 Stroke completed -- waiting for pressure to equalize ... @14:21:36.75 SS.reconfigure :exhale @14:21:36.79 SS.hold @14:21:51.53 Intake.select :close @14:21:51.67 SSV.dial :exhaust @14:21:52.42 Exhaust.select :open @14:21:52.54 SS.seek 0ml @14:21:56.53 Exhaust.select :close @14:21:56.64 Sampling 1000.0ml @14:21:56.65 Intake.select :close @14:21:56.66 Exhaust.select :close @14:21:56.67 SS.reconfigure :default @14:21:56.71 SS.hold @14:21:56.91 CTV.dial :intake @14:21:56.93 SSV.dial CBV @14:21:56.96 CBV.dial SSV @14:21:57.71 Allowing 130s for 25.0ml of remaining 1000.0ml @14:21:57.72 Intake.select :open @14:21:59.87 Intake @15.9psia, vacuum from 10.0 to 13.0psi @14:21:59.89 SS.reconfigure :inhaling 2.20ml/s @14:21:59.94 SS.seek 25ml @14:22:05.77 Vacuum too high -- waiting up to 40 seconds @14:22:05.79 SS.reconfigure :exhale @14:22:05.83 SS.hold @14:22:10.32 Intake @15.5psia, vacuum from 10.0 to 13.0psi @14:22:10.34 SS.reconfigure :inhaling 1.98ml/s @14:22:10.38 SS.seek 25ml @14:22:16.59 Vacuum too high -- waiting up to 40 seconds @14:22:16.60 SS.reconfigure :exhale @14:22:16.64 SS.hold @14:22:23.37 Intake @16.1psia, vacuum from 10.0 to 13.0psi @14:22:23.39 SS.reconfigure :inhaling 1.79ml/s @14:22:23.44 SS.seek 25ml @14:22:28.31 Vacuum too high -- waiting up to 40 seconds @14:22:28.32 SS.reconfigure :exhale @14:22:28.36 SS.hold @14:22:33.39 Intake @16.3psia, vacuum from 10.0 to 13.0psi @14:22:33.41 SS.reconfigure :inhaling 1.61ml/s @14:22:33.46 SS.seek 25ml @14:22:33.75 Stroke completed -- waiting for pressure to equalize ... @14:22:33.76 SS.reconfigure :exhale @14:22:33.80 SS.hold @14:22:37.26 Intake.select :close @14:22:37.40 SSV.dial :exhaust @14:22:38.16 Exhaust.select :open @14:22:38.27 SS.seek 0ml @14:22:47.25 Exhaust.select :close @14:22:47.36 SSV.dial CBV @14:22:47.38 CBV.dial SSV @14:22:48.16 Allowing 130s for 25.0ml of remaining 975.0ml @14:22:48.17 Intake.select :open @14:22:50.33 Intake @16.1psia, vacuum from 10.0 to 13.0psi @14:22:50.35 SS.reconfigure :inhaling 1.61ml/s @14:22:50.40 SS.seek 25ml @14:22:58.49 Vacuum too high -- waiting up to 40 seconds @14:22:58.50 SS.reconfigure :exhale @14:22:59.03 SS.hold @14:23:02.44 Intake @15.6psia, vacuum from 10.0 to 13.0psi @14:23:02.46 SS.reconfigure :inhaling 1.45ml/s @14:23:02.50 SS.seek 25ml @14:23:13.02 Vacuum too high -- waiting up to 40 seconds @14:23:13.04 SS.reconfigure :exhale @14:23:13.08 SS.hold @14:23:20.66 Intake @15.8psia, vacuum from 10.0 to 13.0psi @14:23:20.68 SS.reconfigure :inhaling 1.30ml/s @14:23:20.72 SS.seek 25ml @14:23:22.53 Stroke completed -- waiting for pressure to equalize ... @14:23:22.54 SS.reconfigure :exhale @14:23:22.58 SS.hold @14:23:27.36 Intake.select :close @14:23:27.50 SSV.dial :exhaust @14:23:28.27 Exhaust.select :open @14:23:28.38 SS.seek 0ml @14:23:37.34 Exhaust.select :close @14:23:37.45 SSV.dial CBV @14:23:37.47 CBV.dial SSV @14:23:38.24 Allowing 130s for 25.0ml of remaining 950.0ml @14:23:38.25 Intake.select :open @14:23:40.40 Intake @16.1psia, vacuum from 10.0 to 13.0psi @14:23:40.42 SS.reconfigure :inhaling 1.30ml/s @14:23:40.47 SS.seek 25ml @14:23:50.21 Vacuum too high -- waiting up to 40 seconds @14:23:50.22 SS.reconfigure :exhale @14:23:50.26 SS.hold @14:23:54.50 Intake @15.8psia, vacuum from 10.0 to 13.0psi @14:23:54.52 SS.reconfigure :inhaling 1.17ml/s @14:23:54.56 SS.seek 25ml @14:24:06.52 Vacuum too high -- waiting up to 40 seconds @14:24:06.53 SS.reconfigure :exhale @14:24:06.58 SS.hold @14:24:13.10 Intake @15.6psia, vacuum from 10.0 to 13.0psi @14:24:13.12 SS.reconfigure :inhaling 1.06ml/s @14:24:13.16 SS.seek 25ml @14:24:15.50 Stroke completed -- waiting for pressure to equalize ... @14:24:15.51 SS.reconfigure :exhale @14:24:15.55 SS.hold @14:24:20.93 Intake.select :close @14:24:21.07 SSV.dial :exhaust @14:24:21.83 Exhaust.select :open @14:24:21.94 SS.seek 0ml @14:24:30.90 Exhaust.select :close @14:24:31.01 SSV.dial CBV @14:24:31.04 CBV.dial SSV @14:24:31.78 Allowing 130s for 25.0ml of remaining 925.0ml @14:24:31.80 Intake.select :open @14:24:33.95 Intake @16.2psia, vacuum from 10.0 to 13.0psi @14:24:33.97 SS.reconfigure :inhaling 1.06ml/s @14:24:34.01 SS.seek 25ml @14:24:46.18 Vacuum too high -- waiting up to 40 seconds @14:24:46.19 SS.reconfigure :exhale @14:24:46.23 SS.hold @14:24:50.41 Intake @15.7psia, vacuum from 10.0 to 13.0psi @14:24:50.43 SS.reconfigure :inhaling 0.95ml/s @14:24:50.47 SS.seek 25ml @14:25:03.81 recoverPRVreagent08 @14:25:03.82 PTV.dial :mixing @14:25:04.58 PBV.dial :waste1 @14:25:04.96 delay 30 seconds @14:25:05.86 Stroke completed -- waiting for pressure to equalize ... @14:25:05.87 SS.reconfigure :exhale @14:25:05.91 SS.hold @14:25:18.47 Intake.select :close @14:25:18.62 SSV.dial :exhaust @14:25:19.39 Exhaust.select :open @14:25:19.50 SS.seek 0ml @14:25:28.53 Exhaust.select :close @14:25:28.64 SSV.dial CBV @14:25:28.66 CBV.dial SSV @14:25:29.44 Allowing 130s for 25.0ml of remaining 900.0ml @14:25:29.45 Intake.select :open @14:25:31.60 Intake @16.0psia, vacuum from 10.0 to 13.0psi @14:25:31.62 SS.reconfigure :inhaling 0.95ml/s @14:25:31.67 SS.seek 25ml @14:25:34.97 PBV.dial between PSV and waste1 @14:25:35.35 PSV.dial :air @14:25:35.82 PS.reconfigure :fast @14:25:35.87 PS.seek 2.5ml @14:25:47.05 Vacuum too high -- waiting up to 40 seconds @14:25:47.06 SS.reconfigure :exhale @14:25:47.11 SS.hold @14:25:51.97 Intake @15.7psia, vacuum from 10.0 to 13.0psi @14:25:51.99 SS.reconfigure :inhaling 0.87ml/s @14:25:52.03 SS.seek 25ml @14:25:59.97 PSV.dial :mixloop @14:26:00.44 delay 2 seconds @14:26:02.46 PSV.dial :air @14:26:02.93 delay 2 seconds @14:26:04.94 PSV.dial :mixloop @14:26:05.42 delay 2 seconds @14:26:06.53 Stroke completed -- waiting for pressure to equalize ... @14:26:06.54 SS.reconfigure :exhale @14:26:06.58 SS.hold @14:26:07.43 PSV.dial :air @14:26:07.90 PS.seek 0ml @14:26:18.11 Intake.select :close @14:26:18.25 SSV.dial :exhaust @14:26:19.01 Exhaust.select :open @14:26:19.12 SS.seek 0ml @14:26:27.93 Exhaust.select :close @14:26:28.04 SSV.dial CBV @14:26:28.06 CBV.dial SSV @14:26:28.84 Allowing 130s for 25.0ml of remaining 875.0ml @14:26:28.86 Intake.select :open @14:26:31.01 Intake @15.9psia, vacuum from 10.0 to 13.0psi @14:26:31.03 SS.reconfigure :inhaling 0.87ml/s @14:26:31.07 SS.seek 25ml @14:26:31.32 washCycleDA #1 of 2 @14:26:31.33 PSR.select :air @14:26:31.34 PSV.dial PSR @14:26:32.78 Pulling 0.5ml of PSR.wash @14:26:32.79 PSR.select :wash @14:26:32.95 PS.reconfigure :default @14:26:33.00 PS.seek 0.5ml @14:26:38.94 delay 5 seconds @14:26:43.96 PSR.select :air @14:26:44.08 PS.seek 2ml @14:26:50.77 Vacuum too high -- waiting up to 40 seconds @14:26:50.78 SS.reconfigure :exhale @14:26:50.82 SS.hold @14:26:57.34 Intake @15.5psia, vacuum from 10.0 to 13.0psi @14:26:57.36 SS.reconfigure :inhaling 0.78ml/s @14:26:57.41 SS.seek 25ml @14:27:00.16 PTV.dial :puck @14:27:00.91 PSV.dial PTV @14:27:01.77 PBV.dial :waste1 @14:27:02.19 PS.seek 1.5ml @14:27:08.10 delay 3 seconds @14:27:10.78 Stroke completed -- waiting for pressure to equalize ... @14:27:10.79 SS.reconfigure :exhale @14:27:10.83 SS.hold @14:27:11.11 PBV.dial between PSV and waste1 @14:27:11.49 PS.seek 0ml @14:27:21.88 Intake.select :close @14:27:22.02 SSV.dial :exhaust @14:27:22.78 Exhaust.select :open @14:27:22.89 SS.seek 0ml @14:27:27.60 delay 2:00 @14:27:31.85 Exhaust.select :close @14:27:31.96 SSV.dial CBV @14:27:31.98 CBV.dial SSV @14:27:32.76 Allowing 130s for 25.0ml of remaining 850.0ml @14:27:32.78 Intake.select :open @14:27:34.93 Intake @15.9psia, vacuum from 10.0 to 13.0psi @14:27:34.95 SS.reconfigure :inhaling 0.78ml/s @14:27:34.99 SS.seek 25ml @14:27:58.18 Vacuum too high -- waiting up to 40 seconds @14:27:58.19 SS.reconfigure :exhale @14:27:58.23 SS.hold @14:28:05.70 Intake @15.6psia, vacuum from 10.0 to 13.0psi @14:28:05.72 SS.reconfigure :inhaling 0.70ml/s @14:28:05.76 SS.seek 25ml @14:28:18.01 Stroke completed -- waiting for pressure to equalize ... @14:28:18.02 SS.reconfigure :exhale @14:28:18.06 SS.hold @14:28:29.10 Intake.select :close @14:28:29.24 SSV.dial :exhaust @14:28:30.01 Exhaust.select :open @14:28:30.12 SS.seek 0ml @14:28:38.93 Exhaust.select :close @14:28:39.04 SSV.dial CBV @14:28:39.06 CBV.dial SSV @14:28:39.84 Allowing 130s for 25.0ml of remaining 825.0ml @14:28:39.85 Intake.select :open @14:28:42.01 Intake @15.8psia, vacuum from 10.0 to 13.0psi @14:28:42.03 SS.reconfigure :inhaling 0.70ml/s @14:28:42.07 SS.seek 25ml @14:29:06.24 Vacuum too high -- waiting up to 40 seconds @14:29:06.25 SS.reconfigure :exhale @14:29:06.29 SS.hold @14:29:12.80 Intake @15.1psia, vacuum from 9.6 to 12.6psi @14:29:12.82 SS.reconfigure :inhaling 0.63ml/s @14:29:12.86 SS.seek 25ml @14:29:27.61 recoverPSRreagent08 @14:29:27.63 PTV.dial :puck @14:29:27.64 PBV.dial :waste1 @14:29:28.00 delay 30 seconds @14:29:28.48 Stroke completed -- waiting for pressure to equalize ... @14:29:28.49 SS.reconfigure :exhale @14:29:28.53 SS.hold @14:29:41.09 Intake.select :close @14:29:41.23 SSV.dial :exhaust @14:29:41.99 Exhaust.select :open @14:29:42.10 SS.seek 0ml @14:29:51.07 Exhaust.select :close @14:29:51.18 SSV.dial CBV @14:29:51.20 CBV.dial SSV @14:29:51.98 Allowing 130s for 25.0ml of remaining 800.0ml @14:29:51.99 Intake.select :open @14:29:54.15 Intake @15.8psia, vacuum from 10.0 to 13.0psi @14:29:54.17 SS.reconfigure :inhaling 0.63ml/s @14:29:54.21 SS.seek 25ml @14:29:58.02 PBV.dial between PSV and waste1 @14:29:58.39 PSR.select :air @14:29:58.41 PSV.dial PSR @14:29:59.17 PS.reconfigure :fast @14:29:59.22 PS.seek 2.5ml @14:30:22.95 Vacuum too high -- waiting up to 40 seconds @14:30:22.97 SS.reconfigure :exhale @14:30:23.01 SS.hold @14:30:23.32 PSV.dial PTV @14:30:24.11 delay 5 seconds @14:30:29.15 PSV.dial PSR @14:30:29.92 delay 5 seconds @14:30:31.11 Intake @15.5psia, vacuum from 10.0 to 13.0psi @14:30:31.13 SS.reconfigure :inhaling 0.57ml/s @14:30:31.17 SS.seek 25ml @14:30:34.94 PSV.dial PTV @14:30:35.70 delay 5 seconds @14:30:40.72 PSV.dial :air @14:30:41.60 PS.seek 0ml @14:30:45.62 Stroke completed -- waiting for pressure to equalize ... @14:30:45.63 SS.reconfigure :exhale @14:30:45.67 SS.hold @14:30:59.95 Intake.select :close @14:31:00.10 SSV.dial :exhaust @14:31:00.87 Exhaust.select :open @14:31:00.98 SS.seek 0ml @14:31:04.81 washCycleDA #2 of 2 @14:31:04.82 PSR.select :air @14:31:04.84 PSV.dial PSR @14:31:06.22 Pulling 0.5ml of PSR.wash @14:31:06.23 PSR.select :wash @14:31:06.34 PS.reconfigure :default @14:31:06.39 PS.seek 0.5ml @14:31:10.05 Exhaust.select :close @14:31:10.15 SSV.dial CBV @14:31:10.17 CBV.dial SSV @14:31:10.93 Allowing 130s for 25.0ml of remaining 775.0ml @14:31:10.94 Intake.select :open @14:31:12.34 delay 5 seconds @14:31:13.10 Intake @15.8psia, vacuum from 10.0 to 13.0psi @14:31:13.12 SS.reconfigure :inhaling 0.57ml/s @14:31:13.17 SS.seek 25ml @14:31:17.36 PSR.select :air @14:31:17.47 PS.seek 2ml @14:31:33.68 PTV.dial :puck @14:31:33.70 PSV.dial PTV @14:31:34.45 PBV.dial :waste1 @14:31:34.88 PS.seek 1.5ml @14:31:40.78 delay 3 seconds @14:31:43.80 PBV.dial between PSV and waste1 @14:31:44.16 PS.seek 0ml @14:31:45.39 Vacuum too high -- waiting up to 40 seconds @14:31:45.40 SS.reconfigure :exhale @14:31:45.44 SS.hold @14:31:55.21 Intake @15.8psia, vacuum from 10.0 to 13.0psi @14:31:55.23 SS.reconfigure :inhaling 0.51ml/s @14:31:55.27 SS.seek 25ml @14:32:00.47 delay 2:00 @14:32:10.24 Stroke completed -- waiting for pressure to equalize ... @14:32:10.25 SS.reconfigure :exhale @14:32:10.29 SS.hold @14:32:24.76 Intake.select :close @14:32:24.90 SSV.dial :exhaust @14:32:25.66 Exhaust.select :open @14:32:25.77 SS.seek 0ml @14:32:34.55 Exhaust.select :close @14:32:34.66 SSV.dial CBV @14:32:34.68 CBV.dial SSV @14:32:35.46 Allowing 130s for 25.0ml of remaining 750.0ml @14:32:35.47 Intake.select :open @14:32:37.63 Intake @15.7psia, vacuum from 10.0 to 13.0psi @14:32:37.65 SS.reconfigure :inhaling 0.51ml/s @14:32:37.69 SS.seek 25ml @14:33:15.23 Vacuum too high -- waiting up to 40 seconds @14:33:15.24 SS.reconfigure :exhale @14:33:15.29 SS.hold @14:33:25.12 Intake @15.7psia, vacuum from 10.0 to 13.0psi @14:33:25.14 SS.reconfigure :inhaling 0.47ml/s @14:33:25.18 SS.seek 25ml @14:33:40.22 Stroke completed -- waiting for pressure to equalize ... @14:33:40.23 SS.reconfigure :exhale @14:33:40.28 SS.hold @14:33:56.46 Intake.select :close @14:33:56.61 SSV.dial :exhaust @14:33:57.36 Exhaust.select :open @14:33:57.47 SS.seek 0ml @14:34:00.49 recoverPSRreagent08 @14:34:00.50 PTV.dial :puck @14:34:00.51 PBV.dial :waste1 @14:34:00.86 delay 30 seconds @14:34:06.69 Exhaust.select :close @14:34:06.80 SSV.dial CBV @14:34:06.82 CBV.dial SSV @14:34:07.60 Allowing 130s for 25.0ml of remaining 725.0ml @14:34:07.61 Intake.select :open @14:34:09.77 Intake @15.8psia, vacuum from 10.0 to 13.0psi @14:34:09.79 SS.reconfigure :inhaling 0.47ml/s @14:34:09.83 SS.seek 25ml @14:34:30.87 PBV.dial between PSV and waste1 @14:34:31.23 PSR.select :air @14:34:31.25 PSV.dial PSR @14:34:32.01 PS.reconfigure :fast @14:34:32.06 PS.seek 2.5ml @14:34:53.29 Vacuum too high -- waiting up to 40 seconds @14:34:53.31 SS.reconfigure :exhale @14:34:53.35 SS.hold @14:34:55.90 PSV.dial PTV @14:34:56.67 delay 5 seconds @14:35:01.68 PSV.dial PSR @14:35:02.43 delay 5 seconds @14:35:06.07 SS.reconfigure :inhaling 0.43ml/s @14:35:06.12 SS.seek 25ml @14:35:07.45 PSV.dial PTV @14:35:08.21 delay 5 seconds @14:35:13.23 PSV.dial :air @14:35:13.98 PS.seek 0ml @14:35:18.81 Stroke completed -- waiting for pressure to equalize ... @14:35:18.83 SS.reconfigure :exhale @14:35:18.87 SS.hold @14:35:37.14 Intake.select :close @14:35:37.20 getPMLReagent08 @14:35:37.21 PRV.dial :air @14:35:37.30 SSV.dial :exhaust @14:35:37.71 delay 5 seconds @14:35:38.06 Exhaust.select :open @14:35:38.18 SS.seek 0ml @14:35:42.73 PTV.dial :PRVmixing @14:35:44.06 delay 5 seconds @14:35:47.19 Exhaust.select :close @14:35:47.30 SSV.dial CBV @14:35:47.33 CBV.dial SSV @14:35:48.07 Allowing 130s for 25.0ml of remaining 700.0ml @14:35:48.08 Intake.select :open @14:35:49.07 PRV.dial :ab2 @14:35:49.54 PTV.dial :PRVmixing @14:35:49.55 PSV.dial :mixloop @14:35:50.01 PS.reconfigure :default @14:35:50.06 PS.seek 0.3ml @14:35:50.23 Intake @15.8psia, vacuum from 10.0 to 13.0psi @14:35:50.25 SS.reconfigure :inhaling 0.43ml/s @14:35:50.30 SS.seek 25ml @14:35:53.90 delay 2 seconds @14:35:55.91 PRV.dial :air @14:35:56.44 PS.seek 0.55ml @14:35:59.84 addPMLReagent08 @14:35:59.85 PSV.dial :air @14:36:00.31 PS.seek 2ml @14:36:16.02 PSV.dial :PML @14:36:16.49 PTV.dial :mixing @14:36:17.24 PBV.dial :waste1 @14:36:17.68 PS.seek 1.85ml @14:36:20.02 PBV.dial between PSV and waste1 @14:36:20.41 PS.seek 0ml @14:36:36.38 Vacuum too high -- waiting up to 40 seconds @14:36:36.40 SS.reconfigure :exhale @14:36:36.44 SS.hold @14:36:40.05 delay 2 seconds @14:36:42.07 delay 8:00 @14:36:50.83 Intake @16.0psia, vacuum from 10.0 to 13.0psi @14:36:50.85 SS.reconfigure :inhaling 0.38ml/s @14:36:50.89 SS.seek 25ml @14:37:08.15 Stroke completed -- waiting for pressure to equalize ... @14:37:08.17 SS.reconfigure :exhale @14:37:08.21 SS.hold @14:37:28.79 Intake.select :close @14:37:28.94 SSV.dial :exhaust @14:37:29.69 Exhaust.select :open @14:37:29.80 SS.seek 0ml @14:37:38.87 Exhaust.select :close @14:37:38.98 SSV.dial CBV @14:37:39.00 CBV.dial SSV @14:37:39.78 Allowing 130s for 25.0ml of remaining 675.0ml @14:37:39.80 Intake.select :open @14:37:41.95 Intake @15.9psia, vacuum from 10.0 to 13.0psi @14:37:41.97 SS.reconfigure :inhaling 0.38ml/s @14:37:42.01 SS.seek 25ml @14:38:28.69 Vacuum too high -- waiting up to 40 seconds @14:38:28.71 SS.reconfigure :exhale @14:38:28.75 SS.hold @14:38:43.12 Intake @15.9psia, vacuum from 10.0 to 13.0psi @14:38:43.14 SS.reconfigure :inhaling 0.34ml/s @14:38:43.18 SS.seek 25ml @14:39:08.43 Stroke completed -- waiting for pressure to equalize ... @14:39:08.45 SS.reconfigure :exhale @14:39:08.49 SS.hold @14:39:32.86 Intake.select :close @14:39:33.01 SSV.dial :exhaust @14:39:33.75 Exhaust.select :open @14:39:33.86 SS.seek 0ml @14:39:42.64 Exhaust.select :close @14:39:42.74 SSV.dial CBV @14:39:42.80 CBV.dial SSV @14:39:43.53 Allowing 130s for 25.0ml of remaining 650.0ml @14:39:43.54 Intake.select :open @14:39:45.70 Intake @15.8psia, vacuum from 10.0 to 13.0psi @14:39:45.72 SS.reconfigure :inhaling 0.34ml/s @14:39:45.76 SS.seek 25ml @14:40:50.69 Vacuum too high -- waiting up to 40 seconds @14:40:50.70 SS.reconfigure :exhale @14:40:50.75 SS.hold @14:41:09.61 Intake @16.1psia, vacuum from 10.0 to 13.0psi @14:41:09.63 SS.reconfigure :inhaling 0.31ml/s @14:41:09.67 SS.seek 25ml @14:41:26.48 Stroke completed -- waiting for pressure to equalize ... @14:41:26.49 SS.reconfigure :exhale @14:41:26.53 SS.hold @14:41:52.95 Intake.select :close @14:41:53.09 SSV.dial :exhaust @14:41:53.86 Exhaust.select :open @14:41:53.97 SS.seek 0ml @14:42:02.81 Exhaust.select :close @14:42:02.91 SSV.dial CBV @14:42:02.93 CBV.dial SSV @14:42:03.70 Allowing 130s for 25.0ml of remaining 625.0ml @14:42:03.71 Intake.select :open @14:42:05.88 Intake @15.9psia, vacuum from 10.0 to 13.0psi @14:42:05.89 SS.reconfigure :inhaling 0.31ml/s @14:42:05.94 SS.seek 25ml @14:42:59.60 Vacuum too high -- waiting up to 40 seconds @14:42:59.61 SS.reconfigure :exhale @14:42:59.65 SS.hold @14:43:15.83 Intake @16.0psia, vacuum from 10.0 to 13.0psi @14:43:15.85 SS.reconfigure :inhaling 0.28ml/s @14:43:15.89 SS.seek 25ml @14:43:55.80 Stroke completed -- waiting for pressure to equalize ... @14:43:55.81 SS.reconfigure :exhale @14:43:55.86 SS.hold @14:44:13.72 SS.reconfigure :default @14:44:13.76 SS.hold @14:44:14.42 Intake.select :close @14:44:14.56 ShallowSampler::Clogged in WCR -- Filtering too Slowly after 400.0ml @14:44:14.58 SSV.dial :exhaust @14:44:15.34 Exhaust.select :open @14:44:15.45 SS.reconfigure :exhale @14:44:15.49 SS.seek 0ml @14:44:24.71 Exhaust.select :close @14:44:24.82 Sampled 400.0ml @14:44:24.84 Drying puck before fixing for 20:00 CBV.dial :waste2b @14:44:25.61 delay 1:00 @14:44:42.09 recoverPRVreagent08 @14:44:42.10 PTV.dial :mixing @14:44:42.11 PBV.dial :waste1 @14:44:42.48 delay 30 seconds @14:45:12.51 PBV.dial between PSV and waste1 @14:45:12.87 PSV.dial :air @14:45:13.35 PS.reconfigure :fast @14:45:13.39 PS.seek 2.5ml @14:45:25.63 CSV.dial :air @14:45:26.10 CS.reconfigure :fast @14:45:26.14 CS.seek 10ml @14:45:37.29 PSV.dial :mixloop @14:45:37.76 delay 2 seconds @14:45:39.78 PSV.dial :air @14:45:40.25 delay 2 seconds @14:45:42.26 PSV.dial :mixloop @14:45:42.73 delay 2 seconds @14:45:44.75 PSV.dial :air @14:45:45.22 PS.seek 0ml @14:45:50.64 Pumping wcr puck from tube 4 up to 35psi... @14:45:50.65 CBV.dial SSV @14:45:51.97 CBV.dial between CSV and waste2b @14:45:52.92 CSV.dial between air and CTV @14:45:53.24 CS.seek 7.229ml @14:46:00.33 CTV.dial CSV,avoiding: :air @14:46:00.35 CSV.dial CTV @14:46:01.18 CS.reconfigure :pressurize @14:46:01.22 CS.seek 0ml @14:46:08.58 washCycleDA #1 of 3 @14:46:08.59 PSR.select :air @14:46:08.60 PSV.dial PSR @14:46:09.97 Pulling 0.5ml of PSR.wash @14:46:09.98 PSR.select :wash @14:46:10.09 PS.reconfigure :default @14:46:10.14 PS.seek 0.5ml @14:46:16.01 delay 5 seconds @14:46:17.53 CBV.dial :waste2b @14:46:17.86 CS.reconfigure :default @14:46:17.91 CS.seek 0ml @14:46:21.03 PSR.select :air @14:46:21.15 PS.seek 2ml @14:46:21.26 delay 1:00 @14:46:37.26 PTV.dial :puck @14:46:38.01 PSV.dial PTV @14:46:38.76 PBV.dial :waste1 @14:46:39.19 PS.seek 1.5ml @14:46:45.17 delay 3 seconds @14:46:48.18 PBV.dial between PSV and waste1 @14:46:48.57 PS.seek 0ml @14:47:04.67 delay 2:00 @14:47:21.29 CTV.dial :air,avoiding: :intake @14:47:22.64 Fix #1 of 2 (with RNAlater) for 10:00 @14:47:22.66 CSV.dial CSR @14:47:23.14 Pulling 1.999ml of CSR.RNAlater @14:47:23.16 CSR.select :RNAlater @14:47:23.27 CS.seek 2ml @14:47:29.29 delay 5 seconds @14:47:34.31 CSR.select :air @14:47:34.43 CS.reconfigure :fast @14:47:34.47 CS.seek 10ml @14:47:54.23 Pumping wcr puck from tube 4 up to 35psi... @14:47:54.25 CBV.dial SSV @14:47:55.54 CBV.dial between CSV and waste2b @14:47:56.49 CSV.dial between air and CTV @14:47:57.10 CS.seek 7.113ml @14:48:04.52 CTV.dial CSV,avoiding: :air @14:48:04.54 CSV.dial CTV @14:48:05.92 CS.reconfigure :pressurize @14:48:05.97 CS.seek 0ml @14:48:19.87 delay 10:00 @14:49:04.69 recoverPSRreagent08 @14:49:04.70 PTV.dial :puck @14:49:04.71 PBV.dial :waste1 @14:49:05.56 delay 30 seconds @14:49:35.58 PBV.dial between PSV and waste1 @14:49:35.93 PSR.select :air @14:49:35.95 PSV.dial PSR @14:49:36.72 PS.reconfigure :fast @14:49:36.76 PS.seek 2.5ml @14:50:00.47 PSV.dial PTV @14:50:01.24 delay 5 seconds @14:50:06.26 PSV.dial PSR @14:50:07.01 delay 5 seconds @14:50:12.03 PSV.dial PTV @14:50:12.77 delay 5 seconds @14:50:17.79 PSV.dial :air @14:50:18.67 PS.seek 0ml @14:50:41.82 washCycleDA #2 of 3 @14:50:41.83 PSR.select :air @14:50:41.85 PSV.dial PSR @14:50:43.22 Pulling 0.5ml of PSR.wash @14:50:43.23 PSR.select :wash @14:50:43.34 PS.reconfigure :default @14:50:43.38 PS.seek 0.5ml @14:50:49.32 delay 5 seconds @14:50:54.34 PSR.select :air @14:50:54.45 PS.seek 2ml @14:51:10.63 PTV.dial :puck @14:51:10.64 PSV.dial PTV @14:51:11.51 PBV.dial :waste1 @14:51:11.92 PS.seek 1.5ml @14:51:17.79 delay 3 seconds @14:51:20.81 PBV.dial between PSV and waste1 @14:51:21.17 PS.seek 0ml @14:51:37.48 delay 2:00 @14:53:37.50 recoverPSRreagent08 @14:53:37.51 PTV.dial :puck @14:53:37.52 PBV.dial :waste1 @14:53:37.89 delay 30 seconds @14:54:07.92 PBV.dial between PSV and waste1 @14:54:08.29 PSR.select :air @14:54:08.31 PSV.dial PSR @14:54:09.06 PS.reconfigure :fast @14:54:09.10 PS.seek 2.5ml @14:54:32.81 PSV.dial PTV @14:54:33.58 delay 5 seconds @14:54:38.60 PSV.dial PSR @14:54:39.47 delay 5 seconds @14:54:44.49 PSV.dial PTV @14:54:45.22 delay 5 seconds @14:54:50.24 PSV.dial :air @14:54:50.99 PS.seek 0ml @14:55:14.28 washCycleDA #3 of 3 @14:55:14.29 PSR.select :air @14:55:14.31 PSV.dial PSR @14:55:15.68 Pulling 0.5ml of PSR.wash @14:55:15.69 PSR.select :wash @14:55:15.80 PS.reconfigure :default @14:55:15.85 PS.seek 0.5ml @14:55:21.76 delay 5 seconds @14:55:26.77 PSR.select :air @14:55:26.89 PS.seek 2ml @14:55:43.09 PTV.dial :puck @14:55:43.11 PSV.dial PTV @14:55:43.85 PBV.dial :waste1 @14:55:44.28 PS.seek 1.5ml @14:55:50.28 delay 3 seconds @14:55:53.30 PBV.dial between PSV and waste1 @14:55:53.68 PS.seek 0ml @14:56:09.85 delay 2:00 @14:58:09.87 recoverPSRreagent08 @14:58:09.88 PTV.dial :puck @14:58:09.89 PBV.dial :waste1 @14:58:10.25 delay 30 seconds @14:58:19.90 CBV.dial :waste2b @14:58:20.22 CS.reconfigure :default @14:58:20.27 CS.seek 0ml @14:58:26.12 delay 1:00 @14:58:40.27 PBV.dial between PSV and waste1 @14:58:40.64 PSR.select :air @14:58:40.66 PSV.dial PSR @14:58:41.43 PS.reconfigure :fast @14:58:41.47 PS.seek 2.5ml @14:59:05.12 PSV.dial PTV @14:59:05.89 delay 5 seconds @14:59:10.91 PSV.dial PSR @14:59:11.65 delay 5 seconds @14:59:16.67 PSV.dial PTV @14:59:17.54 delay 5 seconds @14:59:22.56 PSV.dial :air @14:59:23.33 PS.seek 0ml @14:59:26.15 CTV.dial :air,avoiding: :intake @14:59:27.47 Fix #2 of 2 (with RNAlater) for 10:00 @14:59:27.49 CSV.dial CSR @14:59:27.98 Pulling 2ml of CSR.RNAlater @14:59:27.99 CSR.select :RNAlater @14:59:28.10 CS.seek 2ml @14:59:34.25 delay 5 seconds @14:59:39.27 CSR.select :air @14:59:39.38 CS.reconfigure :fast @14:59:39.43 CS.seek 10ml @14:59:46.59 Gate.power :camera,:ON @14:59:46.61 Mixing DA Substrates PSR.select :air @14:59:46.63 PSV.dial PSR @14:59:48.13 Pulling 0.25ml of PSR.sub1 @14:59:48.15 PSR.select :sub1 @14:59:48.37 PS.reconfigure :default @14:59:48.47 PS.seek 0.25ml @14:59:52.01 delay 5 seconds @14:59:57.07 Pulling 0.25ml of PSR.sub2 @14:59:57.08 PSR.select :sub2 @14:59:57.69 PS.seek 0.5ml @14:59:59.47 Pumping wcr puck from tube 4 up to 35psi... @14:59:59.48 CBV.dial SSV @15:00:00.80 CBV.dial between CSV and waste2b @15:00:01.03 delay 5 seconds @15:00:01.76 CSV.dial between air and CTV @15:00:02.39 CS.seek 7.06ml @15:00:06.05 PSR.select :air @15:00:06.15 PS.seek 2ml @15:00:10.07 CTV.dial CSV,avoiding: :air @15:00:10.09 CSV.dial CTV @15:00:11.54 CS.reconfigure :pressurize @15:00:11.58 CS.seek 0ml @15:00:22.28 PTV.dial :puck @15:00:22.29 PSV.dial PTV @15:00:23.03 PBV.dial :waste1 @15:00:23.48 PS.seek 1.5ml @15:00:29.11 delay 10:00 @15:00:29.43 delay 3 seconds @15:00:32.45 PBV.dial between PSV and waste1 @15:00:32.83 PS.seek 0ml @15:00:48.93 delay 10 seconds @15:00:58.96 recoverPSRreagent08 @15:00:58.97 PTV.dial :puck @15:00:58.98 PBV.dial :waste1 @15:00:59.34 delay 30 seconds @15:01:29.37 PBV.dial between PSV and waste1 @15:01:29.73 PSR.select :air @15:01:29.75 PSV.dial PSR @15:01:30.52 PS.reconfigure :fast @15:01:30.56 PS.seek 2.5ml @15:01:54.71 PSV.dial PTV @15:01:55.48 delay 5 seconds @15:02:00.50 PSV.dial PSR @15:02:01.26 delay 5 seconds @15:02:06.28 PSV.dial PTV @15:02:07.17 delay 5 seconds @15:02:12.19 PSV.dial :air @15:02:12.98 PS.seek 0ml @15:02:36.75 Additional air clear @15:02:36.76 PSR.select :air @15:02:36.77 PSV.dial PSR @15:02:38.16 Pulling 2ml of PSR.air @15:02:38.17 PSR.select :air @15:02:38.19 PS.reconfigure :default @15:02:38.23 PS.seek 2ml @15:02:59.52 delay 5 seconds @15:03:04.54 PTV.dial :puck @15:03:04.55 PSV.dial PTV @15:03:05.29 PBV.dial :waste1 @15:03:05.71 PS.seek 1.5ml @15:03:11.62 delay 3 seconds @15:03:14.64 PBV.dial between PSV and waste1 @15:03:15.01 delay 1 second @15:03:16.03 PSV.dial :waste1 @15:03:17.11 PS.seek 0ml @15:03:33.36 Processing Puck Heater at 28C before imaging PH.coast @15:03:33.48 Imaging da puck in Processing Clamp while storing it to tube 3 @15:03:33.65 SC.reconfigure :tight @15:03:33.69 SC.seek 3 @15:03:37.26 SC.configure :limp @15:03:37.46 Elbow.reconfigure :tight @15:03:37.50 Elbow.seek 3 @15:03:39.27 Forearm.reconfigure :tight @15:03:39.32 Forearm.seek 3 + 450 counts @15:03:40.39 SE.reconfigure :pick @15:03:40.44 SE.seek :beamBreak @15:03:42.25 SE.configure :default @15:03:42.39 Elbow.reconfigure :limp @15:03:42.44 Forearm.reconfigure :loose @15:03:42.48 Forearm.seek 3 @15:03:42.69 Elbow.hold @15:03:42.75 Elbow.reconfigure :tight @15:03:42.84 SE.seek 19.11 Pucks below Top Plate @15:03:43.26 Forearm.force -20 @15:03:43.29 Forearm.seek 3 + 304 counts @15:03:43.57 Forearm.reconfigure :tight @15:03:43.61 Forearm.seek 3 + 450 counts @15:03:43.84 SE.seek 20.11 Pucks below Top Plate @15:03:44.46 Forearm.reconfigure :loose @15:03:44.51 Forearm.seek :retract @15:03:45.47 Elbow.seek PC @15:03:48.66 Elbow.reconfigure :loose @15:03:48.75 Forearm.seek PC @15:03:49.80 Hand.close @15:03:50.38 PC.seek :open @15:03:55.19 Forearm.seek :retract @15:03:56.13 Elbow.reconfigure :tight @15:03:56.17 Elbow.seek 3 @15:03:58.72 Forearm.reconfigure :tight @15:03:58.77 Forearm.seek 3 + 450 counts @15:03:59.81 Hand.open @15:04:00.44 SE.seek 19.77 Pucks below Top Plate @15:04:00.70 SE.reconfigure :seal @15:04:00.75 SE.seek 18.8 Pucks below Top Plate @15:04:01.28 Lights, camera, ACTION! @15:04:01.31 PuckCamera: snap -bin=2,2 -auto /var/log/esp/da23mar1515h400ml.tif @15:04:20.80 Waiting >20 seconds for da to relinquish FlushPuck @15:04:34.75 SX HX9/16 (USB:22): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 30.063 seconds /var/log/esp/da23mar1515h400ml.tif: TIFF Upload Complete @15:04:34.77 PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da23mar1515h400ml15s.tif @15:04:50.43 SX HX9/16 (USB:22): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15 seconds /var/log/esp/midres/da23mar1515h400ml15s.tif: TIFF Upload Complete @15:04:50.45 PuckCamera: snap -bin=2,2 20.0 /var/log/esp/midres/da23mar1515h400ml20s.tif @15:05:11.07 SX HX9/16 (USB:22): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 20 seconds /var/log/esp/midres/da23mar1515h400ml20s.tif: TIFF Upload Complete @15:05:11.09 DAfans.email "30.063 sec auto-exposure",Subject: "da23mar1515h400ml images!" @15:05:11.10 Gate.power :camera,:OFF @15:05:11.37 Elbow.seek 3 @15:05:11.55 Forearm.seek 3 + 450 counts @15:05:11.63 SE.reconfigure :gently @15:05:11.68 SE.seek 19.52 Pucks below Top Plate @15:05:12.43 Elbow.reconfigure :limp @15:05:12.47 Forearm.reconfigure :loose @15:05:12.52 Forearm.seek 3 @15:05:12.80 Elbow.hold @15:05:12.87 Elbow.reconfigure :tight @15:05:12.93 SE.reconfigure :default @15:05:12.99 SE.seek 19.7 Pucks below Top Plate @15:05:13.50 Forearm.force -20 @15:05:13.54 Forearm.seek 3 + 317 counts @15:05:13.85 Forearm.reconfigure :tight @15:05:13.89 Forearm.seek 3 + 450 counts @15:05:14.18 SE.seek :down @15:05:15.38 Forearm.reconfigure :loose @15:05:15.48 Forearm.seek :retract @15:05:15.63 SE.stop @15:05:16.53 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @15:05:16.81 Elbow.seek FlushPuck::Garage @15:05:18.70 Elbow.reconfigure :loose @15:05:18.88 Forearm.seek :garage @15:05:19.95 Hand.close @15:05:20.87 Forearm.seek :retract @15:05:21.92 Elbow.reconfigure :tight @15:05:22.00 Elbow.seek PC @15:05:23.39 Elbow.reconfigure :loose @15:05:23.58 Forearm.reconfigure :tight @15:05:23.67 Forearm.seek PC @15:05:25.18 PC.seek :closed @15:05:29.91 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.61 @15:05:31.18 Hand.open @15:05:32.02 Forearm.reconfigure :loose @15:05:32.10 Forearm.seek :retract @15:05:33.09 PSR.select :air @15:05:33.12 PSV.dial PSR @15:05:33.76 Pulling 0.5ml of PSR.flush @15:05:33.78 PSR.select :flush @15:05:33.95 PS.seek 0.5ml @15:05:39.96 delay 5 seconds @15:05:45.00 PSR.select :air @15:05:45.15 PS.seek 2.5ml @15:05:54.37 upsync @15:06:06.36 delay 1 second @15:06:07.38 PRV.dial :waste2,avoiding: :ab2 @15:06:09.24 PTV.dial :PRVmixing @15:06:10.57 PSV.dial :mixloop @15:06:11.73 PS.seek :empty @15:06:38.14 PSR.select :air @15:06:38.16 PSV.dial PSR @15:06:39.37 Pulling 0.5ml of PSR.flush @15:06:39.38 PSR.select :flush @15:06:39.50 PS.seek 0.5ml @15:06:45.49 delay 5 seconds @15:06:50.51 PSR.select :air @15:06:50.66 PS.seek 2.5ml @15:07:11.86 delay 1 second @15:07:12.88 PBV.dial :waste1 @15:07:13.25 PTV.dial :mixing @15:07:14.00 PSV.dial :mixloop @15:07:15.16 PS.seek :empty @15:07:41.49 PSV.dial PSR @15:07:42.52 PSR.select :flush @15:07:42.64 PS.seek 1ml @15:07:53.70 PSR.select :air @15:07:53.82 PS.seek 2.5ml @15:08:09.99 PSV.dial PTV @15:08:10.74 PTV.dial :puck @15:08:11.47 PBV.dial :waste1 @15:08:11.49 PS.seek 0ml @15:08:37.86 PSV.dial PSR @15:08:38.61 PSR.select :flush @15:08:38.73 PS.seek 0.5ml @15:08:44.70 PSR.select :air @15:08:44.82 PS.seek 2.5ml @15:09:06.05 PSV.dial PTV @15:09:06.80 PBV.dial PSV @15:09:07.37 PS.seek 0ml @15:09:33.70 PSV.dial PBV @15:09:34.18 PS.seek 2.5ml @15:10:00.61 PSV.dial :waste1 @15:10:01.35 PS.seek 0ml @15:10:27.70 PSV.dial PSR @15:10:28.17 PSR.select :air @15:10:28.20 PS.seek 2.5ml @15:10:29.13 CBV.dial :waste2b @15:10:29.46 CS.reconfigure :default @15:10:29.51 CS.seek 0ml @15:10:31.46 delay 1:00 @15:10:54.64 PSV.dial PTV @15:10:55.37 PBV.dial PSV @15:10:55.39 PS.seek 0ml @15:11:21.73 PSV.dial PBV @15:11:22.21 PS.seek 2.5ml @15:11:31.48 CTV.dial :air,avoiding: :intake @15:11:32.86 Storing wcr puck in Collection Clamp to tube 3 @15:11:33.24 Elbow.reconfigure :tight @15:11:33.28 Elbow.seek 3 @15:11:35.90 Forearm.reconfigure :tight @15:11:35.94 Forearm.seek 3 + 450 counts @15:11:36.97 SE.reconfigure :pick @15:11:37.02 SE.seek :beamBreak @15:11:38.73 SE.configure :default @15:11:38.88 Elbow.reconfigure :limp @15:11:38.92 Forearm.reconfigure :loose @15:11:38.97 Forearm.seek 3 @15:11:39.23 Elbow.hold @15:11:39.29 Elbow.reconfigure :tight @15:11:39.39 SE.seek 20.11 Pucks below Top Plate @15:11:39.82 Forearm.force -20 @15:11:40.32 Forearm.seek 3 + 309 counts @15:11:40.62 Forearm.reconfigure :tight @15:11:40.66 Forearm.seek 3 + 450 counts @15:11:40.86 SE.seek 21.11 Pucks below Top Plate @15:11:41.51 Forearm.reconfigure :loose @15:11:41.55 Forearm.seek :retract @15:11:42.52 Elbow.seek CC @15:11:44.13 Elbow.reconfigure :loose @15:11:44.22 Forearm.seek CC @15:11:45.18 Hand.close @15:11:45.75 CC.seek :open @15:11:48.61 PSV.dial :waste1 @15:11:49.35 PS.seek 0ml @15:11:50.49 Forearm.seek :retract @15:11:51.47 Elbow.reconfigure :tight @15:11:51.51 Elbow.seek 3 @15:11:53.29 Elbow.reconfigure :limp @15:11:53.35 Forearm.seek 3 @15:11:54.32 Elbow.hold @15:11:54.38 Elbow.reconfigure :tight @15:11:54.43 Hand.open @15:11:55.11 Forearm.force -20 @15:11:55.15 Forearm.seek 3 + 301 counts @15:11:55.44 Forearm.reconfigure :tight @15:11:55.49 Forearm.seek 3 + 450 counts @15:11:55.75 SE.seek :down @15:11:56.88 Forearm.reconfigure :loose @15:11:56.92 Forearm.seek :retract @15:11:57.02 SE.stop @15:11:57.83
Starting finalPurge @15:12:15.73 PSV.dial PSR @15:12:16.20 PSR.select :flush @15:12:16.32 PS.seek 1ml @15:12:17.34
Waiting >20 seconds for psFlush to relinquish FlushPuck @15:12:27.39 PSR.select :air @15:12:27.50 PS.seek 2.5ml @15:12:43.70 PSV.dial PTV @15:12:44.43 PTV.dial :puck @15:12:44.45 PBV.dial :waste1 @15:12:45.00 PS.seek 0ml @15:13:11.54 PSV.dial PSR @15:13:12.29 PSR.select :flush @15:13:12.41 PS.seek 0.5ml @15:13:18.32 PSR.select :air @15:13:18.43 PS.seek 2.5ml @15:13:39.70 PSV.dial PTV @15:13:40.45 PBV.dial PSV @15:13:41.00 PS.seek 0ml @15:14:07.39 PSV.dial PBV @15:14:07.86 PS.seek 2.5ml @15:14:34.23 PSV.dial :waste1 @15:14:35.10 PS.seek 0ml @15:15:01.38 PSV.dial PSR @15:15:01.84 PSR.select :air @15:15:01.86 PS.seek 2.5ml @15:15:28.35 PSV.dial PTV @15:15:29.09 PBV.dial PSV @15:15:29.11 PS.seek 0ml @15:15:55.38 PSV.dial PBV @15:15:55.84 PS.seek 2.5ml @15:16:22.29 PSV.dial :waste1 @15:16:23.03 PS.seek 0ml @15:16:49.58
Loading FlushPuck into Collection Clamp from Processing Clamp @15:16:49.74 Elbow.seek PC @15:16:52.36 Elbow.reconfigure :loose @15:16:52.45 Forearm.seek PC @15:16:53.41 Hand.close @15:16:54.07 PC.seek :open @15:16:58.78 Forearm.seek :retract @15:16:59.78 Elbow.reconfigure :tight @15:16:59.82 Elbow.seek CC @15:17:03.45 Elbow.reconfigure :loose @15:17:03.55 Forearm.reconfigure :tight @15:17:03.59 Forearm.seek CC @15:17:04.81 CC.seek :closed @15:17:09.37 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.30 @15:17:09.44 Hand.open @15:17:10.06 Forearm.reconfigure :loose @15:17:10.10 Forearm.seek :retract @15:17:11.06 Flushing the Collection Valve::Manifold @15:17:11.17 CSV.dial CSR @15:17:11.66 Pulling 4.999ml of CSR.flush @15:17:11.67 CSR.select :flush @15:17:11.78 CS.seek 5ml @15:17:25.39 delay 5 seconds @15:17:30.41 CSR.select :air @15:17:30.55 CS.reconfigure :fast @15:17:30.59 CS.seek 7.499ml @15:17:37.51 CSV.dial :waste @15:17:38.78 CS.reconfigure :default @15:17:38.83 CS.seek 0ml @15:17:58.80 Flushing lower manifold's waste1 port @15:17:58.91 CSV.dial :flush @15:17:59.35 CS.reconfigure :fast @15:17:59.39 CS.seek 5ml @15:18:12.23 delay 5 seconds @15:18:17.25 CSV.dial :air @15:18:17.87 CS.seek 7ml @15:18:23.36 CSV.dial CTV @15:18:23.38
CTV.dial CSV @15:18:24.78 CBV.dial :waste1 @15:18:25.53 CS.seek 0ml @15:18:42.30 Flushing lower manifold's waste2 port @15:18:42.41 CSV.dial :flush @15:18:43.13 CS.seek 5ml @15:18:55.95 delay 5 seconds @15:19:00.97 CSV.dial :air @15:19:01.48 CS.seek 7ml @15:19:07.20 CSV.dial CTV @15:19:07.22
CTV.dial CSV @15:19:07.67 CBV.dial :waste2 @15:19:08.72 CS.seek 0ml @15:19:25.26 Flushing lower manifold's waste2b port @15:19:25.38 CSV.dial :flush @15:19:26.11 CS.seek 5ml @15:19:38.95 delay 5 seconds @15:19:43.97 CSV.dial :air @15:19:44.48 CS.seek 7.001ml @15:19:49.95 CSV.dial CTV @15:19:49.97
CTV.dial CSV @15:19:50.42 CBV.dial :waste2b @15:19:51.47 CS.seek 0ml @15:20:08.23 Disinfecting external Intake with kill @15:20:08.34 CSV.dial CSR @15:20:08.83 Pulling 8ml of CSR.kill @15:20:08.84 CSR.select :kill @15:20:08.95 CS.seek 8ml @15:20:28.70 delay 5 seconds @15:20:33.72 CSR.select :air @15:20:33.83 CS.seek 10ml @15:20:39.43 CSV.dial CBV @15:20:39.44
CBV.dial CSV @15:20:40.48 CTV.dial :intake @15:20:41.26 Intake.select :open @15:20:41.37 CS.seek 2ml @15:21:00.29 Intake.select :close @15:21:00.39 CTV.dial :air @15:21:01.15 CS.reconfigure :default @15:21:01.20 CS.seek 0ml @15:21:07.13 CSV.dial CSR @15:21:08.24 Pulling 5ml of CSR.flush @15:21:08.25 CSR.select :flush @15:21:08.38 CS.seek 5ml @15:21:22.11 delay 5 seconds @15:21:27.12 CSR.select :air @15:21:27.24 CS.seek 10ml @15:21:40.79 CSV.dial CBV @15:21:41.78 CS.seek 0ml @15:22:08.20 Garaging FlushPuck in Collection Clamp @15:22:08.74 Elbow.reconfigure :tight @15:22:08.78 Elbow.seek CC @15:22:08.89 Elbow.reconfigure :loose @15:22:08.98 Forearm.seek CC @15:22:09.92 Hand.close @15:22:10.49 CC.seek :open @15:22:15.12 Forearm.seek :retract @15:22:16.13 Elbow.reconfigure :tight @15:22:16.17 Elbow.seek :garage @15:22:19.08 Elbow.reconfigure :loose @15:22:19.16 Forearm.reconfigure :tight @15:22:19.20 Forearm.seek :garage @15:22:20.24 Hand.open @15:22:20.92 Forearm.reconfigure :loose @15:22:20.96 Forearm.seek :retract @15:22:21.94 Completed [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] begun 13:13:48 @15:22:21.99 Asleep after completing DA_WCR_031523 assays @15:22:22.01 GoodNews.email "{1 => 33,\n 2 => 33,\n 3 => 22,\n 4 => 11,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@15:12:58, 16% flow, 19.7C, 40% humidity, 14.8psia, 13.744V, 0.309A, 0.290A avg, 4.24W\nSampled 400.0ml",Subject: "Asleep after completing DA_WCR_031523 assays" @15:22:27.17 upsync @12:54:44.88UTC16-Mar-23
ESP2 v3.41 I2C Gateway v3.20 for core bus @12:54:47.43 Configuration for short puck jake 1/11/21 by brent@mbari.org /home/esp/esp2/type/shallow/jake/configure.rb @12:54:51.37 Polling Can every 10:00 @12:54:51.62 Cleanly exited at 12:52:21UTC16-Mar-23 @12:54:51.66 Loading /home/esp/esp2/mission/DA_WCR_031623.rb mission @12:54:51.71 Preparing to sleep until + 00:00:05 @12:54:51.73 GoodNews.email "Starting DA_WCR_031623 w/pucks in tube 4\n{1 => 33,\n 2 => 33,\n 3 => 22,\n 4 => 11,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@12:54:51, 15% flow, 19.5C, 41% humidity, 14.8psia, 13.754V, 0.254A, 0.236A avg, 3.50W",Subject: "DA_WCR_031623 begun" @12:54:51.77
Asleep til + 00:00:05 @12:54:51.81 Continuing at 12:54 @12:54:52.05 Gate.power :core,:OFF @12:54:52.07 Gate.power :raw,:OFF @12:54:52.08 Shutdown @12:54:55.60 GoodNews.email "Then, in tube 4: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2/mission/DA_WCR_031623.rb:14\n{1 => 33,\n 2 => 33,\n 3 => 22,\n 4 => 11,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@12:54:51, 15% flow, 19.5C, 41% humidity, 14.8psia, 13.754V, 0.254A, 0.236A avg, 3.50W",Subject: "Asleep until 12:54" @12:54:56.68
Powering core bus @12:54:57.39 upsync @12:55:00.46 upsync @12:55:02.23
Gate.power :raw,:ON @12:55:02.84 CS.configure :default @12:55:02.93 PS.configure :default @12:55:03.02 SS.configure :default @12:55:03.10 CC.configure :default @12:55:03.19 PC.configure :default @12:55:03.30 Forearm.configure :loose @12:55:03.38 Elbow.configure :loose @12:55:03.49 SC.configure :loose @12:55:03.58 SE.configure :default @12:55:03.62 CH.coast @12:55:03.67 CH.configure :default @12:55:03.70 PH.coast @12:55:03.75 PH.configure :default @12:55:03.78 All dwarf controllers running firmware version 3.85 @12:55:03.89 Valve.reset @12:55:04.17 PSV.dial :air @12:55:04.21 Intake.select :close @12:55:04.23 Exhaust.select :close @12:55:04.24 CTV.dial :air @12:55:04.38 CSV.dial :waste1 @12:55:04.40
Forearm.reconfigure :loose @12:55:04.47 Forearm.home.seek :retract @12:55:04.87 SSV.dial :spit,avoiding: :exhaust @12:55:05.19 Hand.close @12:55:05.22
Elbow.jog 1500 @12:55:05.31 PS.reconfigure :default @12:55:05.36 PS.home.seek 0ml @12:55:05.69 CS.reconfigure :fast @12:55:05.72 SS.reconfigure :default @12:55:05.81 CS.home.seek 0ml @12:55:05.84 SS.home.seek 0ml @12:55:05.95
Elbow.reconfigure :loose @12:55:06.00 Elbow.home.seek :clear @12:55:09.28 SE.reconfigure :default @12:55:09.32 SE.home.seek :down @12:55:09.92 SC.reconfigure :tight @12:55:09.98 SC.home.seek :initial @12:55:13.65 PS.stop @12:55:38.29
SC.configure :limp @12:55:38.34 Forearm.seek :retract @12:55:38.39 Elbow.seek :hammer @12:55:39.51 Forearm.reconfigure :tight @12:55:39.56 Forearm.seek retracted @12:55:39.77 PC.reconfigure :default @12:55:39.79
CC.reconfigure :default @12:55:39.88 CC.home.seek :open @12:55:39.90 PC.home.seek :open @12:55:43.41
Phase [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] @12:55:43.43 Notice.email "At 12:55:43 began in tube 4: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2/mission/DA_WCR_031623.rb:14\n{1 => 33,\n 2 => 33,\n 3 => 22,\n 4 => 11,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@12:55:43, 16% flow, 19.5C, 41% humidity, 14.8psia, 13.715V, 0.296A, 0.278A avg, 4.07W",Subject: "Started DA_WCR_031623 phase initialPurge; da [1000, 1000] #da and wsr are run simultanelously" @12:55:43.45
Starting initialPurge @12:55:43.48 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @12:55:43.54 Hand.open @12:55:44.24 Elbow.reconfigure :tight @12:55:44.29 Elbow.seek FlushPuck::Garage @12:55:46.12 Elbow.reconfigure :loose @12:55:46.20 Forearm.reconfigure :loose @12:55:46.24 Forearm.seek :garage @12:55:47.19 Hand.close @12:55:47.87 Forearm.seek :retract @12:55:48.27 upsync @12:55:48.91
Elbow.reconfigure :tight @12:55:48.95 Elbow.seek CC @12:55:51.91 Elbow.reconfigure :loose @12:55:52.00 Forearm.reconfigure :tight @12:55:52.04 Forearm.seek CC @12:55:53.27 CC.seek :closed @12:55:57.79 Collection Clamp onSpring/offSpring for 13/27 samples, on/off torque 2.35 @12:55:57.86 Hand.open @12:55:58.56 Forearm.reconfigure :loose @12:55:58.60 Forearm.seek :retract @12:55:59.60 Flushing the Collection Valve::Manifold @12:55:59.71 CSV.dial CSR @12:56:01.03 Pulling 5ml of CSR.flush @12:56:01.04 CSR.select :flush @12:56:01.15 CS.reconfigure :default @12:56:01.19 CS.seek 5ml @12:56:15.01 delay 5 seconds @12:56:20.03 CSR.select :air @12:56:20.18 CS.reconfigure :fast @12:56:20.22 CS.seek 7.501ml @12:56:27.23 CSV.dial :waste @12:56:28.50 CS.reconfigure :default @12:56:28.54 CS.seek 0ml @12:56:48.61 Purging external Intake @12:56:48.72 CSV.dial :flush @12:56:49.16 CS.reconfigure :fast @12:56:49.25 CS.seek 10ml @12:57:13.70 delay 5 seconds @12:57:18.72 CSV.dial CBV @12:57:18.75
CBV.dial CSV @12:57:19.52 CTV.dial :intake @12:57:20.31 Intake.select :open @12:57:20.41 CS.seek 0ml @12:57:44.13 Intake.select :close @12:57:44.23 CTV.dial :air @12:57:45.03 Flushing upper manifold @12:57:45.14 CSV.dial :flush @12:57:45.88 CS.seek 5ml @12:57:58.57 delay 5 seconds @12:58:03.59 CSV.dial :air @12:58:04.21 CS.seek 7ml @12:58:09.79 CSV.dial CBV @12:58:09.81
CBV.dial CSV @12:58:10.82 CTV.dial :air @12:58:10.84 CS.seek 0ml @12:58:27.51 delay 7 seconds @12:58:34.54 CS.seek 3ml @12:58:42.54 CSV.dial :waste @12:58:42.98 CS.seek 0ml @12:58:50.73 CSV.dial :flush @12:58:51.17 CS.seek 3ml @12:58:59.13 delay 5 seconds @12:59:04.15 CSV.dial :air @12:59:04.65 CS.seek 5ml @12:59:10.13 CSV.dial :air @12:59:10.15 CS.seek 0ml @12:59:22.32 delay 7 seconds @12:59:29.35 CS.seek 3ml @12:59:37.32 CSV.dial :waste @12:59:38.06 CS.seek 0ml @12:59:45.70 Garaging FlushPuck in Collection Clamp @12:59:45.76 Elbow.reconfigure :tight @12:59:45.80 Elbow.seek CC @12:59:46.08 Elbow.reconfigure :loose @12:59:46.17 Forearm.seek CC @12:59:47.17 Hand.close @12:59:47.75 CC.seek :open @12:59:52.39 Forearm.seek :retract @12:59:53.33 Elbow.reconfigure :tight @12:59:53.37 Elbow.seek :garage @12:59:56.27 Elbow.reconfigure :loose @12:59:56.35 Forearm.reconfigure :tight @12:59:56.39 Forearm.seek :garage @12:59:57.40 Hand.open @12:59:58.49 Forearm.reconfigure :loose @12:59:58.53 Forearm.seek :retract @12:59:59.51 da sampling at most 1000ml, wcr at most 1000ml @12:59:59.55 Loading sh1 puck from tube 4 into Collection Clamp @12:59:59.56 DAfans.email "Started da in tube 4 at 12:59:59UTC16-Mar-23\n{1 => 33,\n 2 => 33,\n 3 => 22,\n 4 => 11,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@12:55:43, 16% flow, 19.5C, 41% humidity, 14.8psia, 13.715V, 0.296A, 0.278A avg, 4.07W",Subject: "da sampling at most 1000ml, wcr at most 1000ml" @13:00:00.03
Elbow.reconfigure :tight @13:00:00.09 Elbow.seek 4 @13:00:02.02 Forearm.reconfigure :tight @13:00:02.06 Forearm.seek 4 + 450 counts @13:00:03.24 SE.reconfigure :pick @13:00:03.29 SE.seek :beamBreak @13:00:04.71 upsync @13:00:05.82
SE.configure :default @13:00:05.97 Elbow.seek 4 @13:00:06.14 Forearm.seek 4 + 300 counts @13:00:06.29 Forearm.reconfigure :loose @13:00:06.33 Hand.close @13:00:07.01 SE.seek :down @13:00:07.19
Forearm.seek :retract @13:00:08.14 Elbow.seek CC @13:00:09.16 SE.stop @13:00:09.69
Elbow.reconfigure :loose @13:00:09.78 Forearm.reconfigure :tight @13:00:09.82 Forearm.seek CC @13:00:11.07 CC.seek :closed @13:00:15.59 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.20 @13:00:15.60 Hand.open @13:00:16.30 Forearm.reconfigure :loose @13:00:16.34 Forearm.seek :retract @13:00:17.30 collectSample 1000ml w/bubble point of 24psi @13:00:17.31 Priming 7ml @13:00:17.32 Intake.select :close @13:00:17.33 Exhaust.select :close @13:00:17.34 SS.hold @13:00:17.53 Calibrating pressure and temperature sensors @13:00:17.56 CTV.dial :air @13:00:17.57 SSV.dial :spit,avoiding: :exhaust @13:00:17.59 SS.reconfigure :fast @13:00:17.63 SS.seek 5ml @13:00:20.65 SSV.dial CBV @13:00:20.68
CBV.dial SSV @13:00:21.98 delay 3.8 seconds @13:00:25.88 SSV.dial :spit @13:00:27.20 SS.seek 0ml @13:00:29.78 SSV.dial CBV @13:00:31.07 CTV.dial :intake @13:00:31.87 SSV.dial CBV @13:00:31.89
CBV.dial SSV @13:00:31.90 Allowing 40s for 7.0ml of remaining 7.0ml @13:00:31.91 Intake.select :open @13:00:34.06 Intake @15.2psia, vacuum from 9.7 to 12.7psi @13:00:34.08 SS.reconfigure :inhaling 2.20ml/s @13:00:34.12 SS.seek 7ml @13:00:41.33 Stroke completed -- waiting for pressure to equalize ... @13:00:41.34 SS.reconfigure :exhale @13:00:41.38 SS.hold @13:00:42.23 Intake.select :close @13:00:42.37 SSV.dial :exhaust @13:00:43.11 Exhaust.select :open @13:00:43.22 SS.seek 0ml @13:00:47.05 Exhaust.select :close @13:00:47.15 Sampling 1000.0ml @13:00:47.16 Intake.select :close @13:00:47.17 Exhaust.select :close @13:00:47.18 SS.reconfigure :default @13:00:47.22 SS.hold @13:00:47.40 CTV.dial :intake @13:00:47.41 SSV.dial CBV @13:00:47.43
CBV.dial SSV @13:00:48.21 Allowing 130s for 25.0ml of remaining 1000.0ml @13:00:48.22 Intake.select :open @13:00:50.37 Intake @15.2psia, vacuum from 9.7 to 12.7psi @13:00:50.38 SS.reconfigure :inhaling 2.20ml/s @13:00:50.42 SS.seek 25ml @13:01:05.52 Stroke completed -- waiting for pressure to equalize ... @13:01:05.53 SS.reconfigure :exhale @13:01:05.57 SS.hold @13:01:14.01 Intake.select :close @13:01:14.15 SSV.dial :exhaust @13:01:14.91 Exhaust.select :open @13:01:15.02 SS.seek 0ml @13:01:23.81 Exhaust.select :close @13:01:23.92 SSV.dial CBV @13:01:23.97
CBV.dial SSV @13:01:24.69 Allowing 130s for 25.0ml of remaining 975.0ml @13:01:24.70 Intake.select :open @13:01:26.85 Intake @15.9psia, vacuum from 10.0 to 13.0psi @13:01:26.87 SS.reconfigure :inhaling 2.20ml/s @13:01:26.91 SS.seek 25ml @13:01:36.23 Vacuum too high -- waiting up to 40 seconds @13:01:36.24 SS.reconfigure :exhale @13:01:36.28 SS.hold @13:01:39.06 Intake @14.0psia, vacuum from 8.5 to 11.5psi @13:01:39.07 SS.reconfigure :inhaling 1.98ml/s @13:01:39.11 SS.seek 25ml @13:01:46.11 Stroke completed -- waiting for pressure to equalize ... @13:01:46.12 SS.reconfigure :exhale @13:01:46.16 SS.hold @13:01:52.54 Intake.select :close @13:01:52.67 SSV.dial :exhaust @13:01:53.42 Exhaust.select :open @13:01:53.53 SS.seek 0ml @13:02:02.55 Exhaust.select :close @13:02:02.65 SSV.dial CBV @13:02:02.69
CBV.dial SSV @13:02:03.44 Allowing 130s for 25.0ml of remaining 950.0ml @13:02:03.45 Intake.select :open @13:02:05.60 Intake @16.1psia, vacuum from 10.0 to 13.0psi @13:02:05.62 SS.reconfigure :inhaling 1.98ml/s @13:02:05.66 SS.seek 25ml @13:02:14.54 Vacuum too high -- waiting up to 40 seconds @13:02:14.55 SS.reconfigure :exhale @13:02:14.59 SS.hold @13:02:15.77 Intake @13.6psia, vacuum from 8.1 to 11.1psi @13:02:15.79 SS.reconfigure :inhaling 1.79ml/s @13:02:15.83 SS.seek 25ml @13:02:23.98 Stroke completed -- waiting for pressure to equalize ... @13:02:23.99 SS.reconfigure :exhale @13:02:24.03 SS.hold @13:02:32.47 Intake.select :close @13:02:32.60 SSV.dial :exhaust @13:02:33.36 Exhaust.select :open @13:02:33.46 SS.seek 0ml @13:02:42.22 Exhaust.select :close @13:02:42.32 SSV.dial CBV @13:02:42.34
CBV.dial SSV @13:02:43.11 Allowing 130s for 25.0ml of remaining 925.0ml @13:02:43.12 Intake.select :open @13:02:45.27 Intake @16.0psia, vacuum from 10.0 to 13.0psi @13:02:45.29 SS.reconfigure :inhaling 1.79ml/s @13:02:45.33 SS.seek 25ml @13:02:55.44 Vacuum too high -- waiting up to 40 seconds @13:02:55.45 SS.reconfigure :exhale @13:02:55.49 SS.hold @13:02:58.65 Intake @14.5psia, vacuum from 9.0 to 12.0psi @13:02:58.67 SS.reconfigure :inhaling 1.61ml/s @13:02:58.71 SS.seek 25ml @13:03:07.28 Stroke completed -- waiting for pressure to equalize ... @13:03:07.29 SS.reconfigure :exhale @13:03:07.33 SS.hold @13:03:14.69 Intake.select :close @13:03:14.83 SSV.dial :exhaust @13:03:15.58 Exhaust.select :open @13:03:15.69 SS.seek 0ml @13:03:24.73 Exhaust.select :close @13:03:24.84 SSV.dial CBV @13:03:24.85
CBV.dial SSV @13:03:25.61 Allowing 130s for 25.0ml of remaining 900.0ml @13:03:25.62 Intake.select :open @13:03:27.77 Intake @16.0psia, vacuum from 10.0 to 13.0psi @13:03:27.79 SS.reconfigure :inhaling 1.61ml/s @13:03:27.83 SS.seek 25ml @13:03:39.13 Vacuum too high -- waiting up to 40 seconds @13:03:39.14 SS.reconfigure :exhale @13:03:39.18 SS.hold @13:03:42.35 Intake @14.5psia, vacuum from 9.0 to 12.0psi @13:03:42.36 SS.reconfigure :inhaling 1.45ml/s @13:03:42.40 SS.seek 25ml @13:03:51.06 Stroke completed -- waiting for pressure to equalize ... @13:03:51.07 SS.reconfigure :exhale @13:03:51.11 SS.hold @13:03:59.54 Intake.select :close @13:03:59.68 SSV.dial :exhaust @13:04:00.42 Exhaust.select :open @13:04:00.53 SS.seek 0ml @13:04:09.50 Exhaust.select :close @13:04:09.60 SSV.dial CBV @13:04:09.62
CBV.dial SSV @13:04:10.37 Allowing 130s for 25.0ml of remaining 875.0ml @13:04:10.38 Intake.select :open @13:04:12.54 Intake @15.9psia, vacuum from 10.0 to 13.0psi @13:04:12.55 SS.reconfigure :inhaling 1.45ml/s @13:04:12.59 SS.seek 25ml @13:04:25.07 Vacuum too high -- waiting up to 40 seconds @13:04:25.08 SS.reconfigure :exhale @13:04:25.12 SS.hold @13:04:29.38 Intake @14.8psia, vacuum from 9.3 to 12.3psi @13:04:29.39 SS.reconfigure :inhaling 1.30ml/s @13:04:29.44 SS.seek 25ml @13:04:37.87 Stroke completed -- waiting for pressure to equalize ... @13:04:37.88 SS.reconfigure :exhale @13:04:37.92 SS.hold @13:04:46.37 Intake.select :close @13:04:46.50 SSV.dial :exhaust @13:04:47.25 Exhaust.select :open @13:04:47.35 SS.seek 0ml @13:04:56.26 Exhaust.select :close @13:04:56.36 SSV.dial CBV @13:04:56.38
CBV.dial SSV @13:04:57.14 Allowing 130s for 25.0ml of remaining 850.0ml @13:04:57.15 Intake.select :open @13:04:59.31 Intake @16.1psia, vacuum from 10.0 to 13.0psi @13:04:59.32 SS.reconfigure :inhaling 1.30ml/s @13:04:59.36 SS.seek 25ml @13:05:10.41 Vacuum too high -- waiting up to 40 seconds @13:05:10.42 SS.reconfigure :exhale @13:05:10.46 SS.hold @13:05:14.07 Intake @15.0psia, vacuum from 9.5 to 12.5psi @13:05:14.09 SS.reconfigure :inhaling 1.17ml/s @13:05:14.13 SS.seek 25ml @13:05:26.06 Stroke completed -- waiting for pressure to equalize ... @13:05:26.07 SS.reconfigure :exhale @13:05:26.11 SS.hold @13:05:37.10 Intake.select :close @13:05:37.26 SSV.dial :exhaust @13:05:38.00 Exhaust.select :open @13:05:38.10 SS.seek 0ml @13:05:46.92 Exhaust.select :close @13:05:47.02 SSV.dial CBV @13:05:47.04
CBV.dial SSV @13:05:47.79 Allowing 130s for 25.0ml of remaining 825.0ml @13:05:47.80 Intake.select :open @13:05:49.95 Intake @16.1psia, vacuum from 10.0 to 13.0psi @13:05:49.97 SS.reconfigure :inhaling 1.17ml/s @13:05:50.01 SS.seek 25ml @13:06:01.60 Vacuum too high -- waiting up to 40 seconds @13:06:02.09 SS.reconfigure :exhale @13:06:02.14 SS.hold @13:06:06.29 Intake @15.5psia, vacuum from 10.0 to 13.0psi @13:06:06.31 SS.reconfigure :inhaling 1.06ml/s @13:06:06.35 SS.seek 25ml @13:06:20.84 Stroke completed -- waiting for pressure to equalize ... @13:06:20.85 SS.reconfigure :exhale @13:06:20.89 SS.hold @13:06:33.51 Intake.select :close @13:06:33.65 SSV.dial :exhaust @13:06:34.39 Exhaust.select :open @13:06:34.49 SS.seek 0ml @13:06:43.54 Exhaust.select :close @13:06:43.64 SSV.dial CBV @13:06:43.66
CBV.dial SSV @13:06:44.42 Allowing 130s for 25.0ml of remaining 800.0ml @13:06:44.43 Intake.select :open @13:06:46.58 Intake @16.0psia, vacuum from 10.0 to 13.0psi @13:06:46.59 SS.reconfigure :inhaling 1.06ml/s @13:06:46.64 SS.seek 25ml @13:06:59.09 Vacuum too high -- waiting up to 40 seconds @13:06:59.10 SS.reconfigure :exhale @13:06:59.15 SS.hold @13:07:04.76 Intake @15.5psia, vacuum from 10.0 to 13.0psi @13:07:04.77 SS.reconfigure :inhaling 0.95ml/s @13:07:04.81 SS.seek 25ml @13:07:19.76 Stroke completed -- waiting for pressure to equalize ... @13:07:19.77 SS.reconfigure :exhale @13:07:19.81 SS.hold @13:07:37.99 Intake.select :close @13:07:38.12 SSV.dial :exhaust @13:07:38.87 Exhaust.select :open @13:07:38.97 SS.seek 0ml @13:07:47.77 Exhaust.select :close @13:07:47.88 SSV.dial CBV @13:07:47.92
CBV.dial SSV @13:07:48.64 Allowing 130s for 25.0ml of remaining 775.0ml @13:07:48.65 Intake.select :open @13:07:50.80 Intake @15.9psia, vacuum from 10.0 to 13.0psi @13:07:50.82 SS.reconfigure :inhaling 0.95ml/s @13:07:50.86 SS.seek 25ml @13:08:03.34 Vacuum too high -- waiting up to 40 seconds @13:08:03.35 SS.reconfigure :exhale @13:08:03.39 SS.hold @13:08:09.85 Intake @15.3psia, vacuum from 9.8 to 12.8psi @13:08:09.87 SS.reconfigure :inhaling 0.87ml/s @13:08:09.91 SS.seek 25ml @13:08:24.40 Vacuum too high -- waiting up to 40 seconds @13:08:24.41 SS.reconfigure :exhale @13:08:24.46 SS.hold @13:08:36.06 Intake @15.6psia, vacuum from 10.0 to 13.0psi @13:08:36.07 SS.reconfigure :inhaling 0.78ml/s @13:08:36.12 SS.seek 25ml @13:08:40.76 Stroke completed -- waiting for pressure to equalize ... @13:08:40.77 SS.reconfigure :exhale @13:08:40.81 SS.hold @13:08:54.26 Intake.select :close @13:08:54.39 SSV.dial :exhaust @13:08:55.13 Exhaust.select :open @13:08:55.24 SS.seek 0ml @13:09:03.94 Exhaust.select :close @13:09:04.05 SSV.dial CBV @13:09:04.07
CBV.dial SSV @13:09:04.82 Allowing 130s for 25.0ml of remaining 750.0ml @13:09:04.83 Intake.select :open @13:09:06.99 Intake @15.8psia, vacuum from 10.0 to 13.0psi @13:09:07.01 SS.reconfigure :inhaling 0.78ml/s @13:09:07.05 SS.seek 25ml @13:09:22.87 Vacuum too high -- waiting up to 40 seconds @13:09:22.88 SS.reconfigure :exhale @13:09:22.92 SS.hold @13:09:31.46 Intake @15.4psia, vacuum from 9.9 to 12.9psi @13:09:31.47 SS.reconfigure :inhaling 0.70ml/s @13:09:31.51 SS.seek 25ml @13:09:48.93 Vacuum too high -- waiting up to 40 seconds @13:09:48.94 SS.reconfigure :exhale @13:09:48.99 SS.hold @13:10:05.23 Intake @15.7psia, vacuum from 10.0 to 13.0psi @13:10:05.25 SS.reconfigure :inhaling 0.63ml/s @13:10:05.29 SS.seek 25ml @13:10:09.86 Stroke completed -- waiting for pressure to equalize ... @13:10:09.87 SS.reconfigure :exhale @13:10:09.91 SS.hold @13:10:26.10 Intake.select :close @13:10:26.24 SSV.dial :exhaust @13:10:26.98 Exhaust.select :open @13:10:27.08 SS.seek 0ml @13:10:36.14 Exhaust.select :close @13:10:36.25 SSV.dial CBV @13:10:36.28
CBV.dial SSV @13:10:37.02 Allowing 130s for 25.0ml of remaining 725.0ml @13:10:37.03 Intake.select :open @13:10:39.18 Intake @15.8psia, vacuum from 10.0 to 13.0psi @13:10:39.20 SS.reconfigure :inhaling 0.63ml/s @13:10:39.24 SS.seek 25ml @13:10:57.15 Vacuum too high -- waiting up to 40 seconds @13:10:57.16 SS.reconfigure :exhale @13:10:57.20 SS.hold @13:11:08.87 Intake @15.5psia, vacuum from 10.0 to 13.0psi @13:11:08.89 SS.reconfigure :inhaling 0.57ml/s @13:11:08.93 SS.seek 25ml @13:11:29.65 Vacuum too high -- waiting up to 40 seconds @13:11:29.66 SS.reconfigure :exhale @13:11:29.70 SS.hold @13:11:48.00 Intake @15.8psia, vacuum from 10.0 to 13.0psi @13:11:48.01 SS.reconfigure :inhaling 0.51ml/s @13:11:48.05 SS.seek 25ml @13:11:54.66 Stroke completed -- waiting for pressure to equalize ... @13:11:54.67 SS.reconfigure :exhale @13:11:54.71 SS.hold @13:12:19.13 Intake.select :close @13:12:19.26 SSV.dial :exhaust @13:12:20.00 Exhaust.select :open @13:12:20.11 SS.seek 0ml @13:12:28.89 Exhaust.select :close @13:12:29.00 SSV.dial CBV @13:12:29.05
CBV.dial SSV @13:12:29.75 Allowing 130s for 25.0ml of remaining 700.0ml @13:12:29.76 Intake.select :open @13:12:31.91 Intake @15.8psia, vacuum from 10.0 to 13.0psi @13:12:31.93 SS.reconfigure :inhaling 0.51ml/s @13:12:31.97 SS.seek 25ml @13:12:52.18 Vacuum too high -- waiting up to 40 seconds @13:12:52.19 SS.reconfigure :exhale @13:12:52.23 SS.hold @13:13:06.64 Intake @15.7psia, vacuum from 10.0 to 13.0psi @13:13:06.66 SS.reconfigure :inhaling 0.47ml/s @13:13:06.70 SS.seek 25ml @13:13:23.09 Vacuum too high -- waiting up to 40 seconds @13:13:23.10 SS.reconfigure :exhale @13:13:23.14 SS.hold @13:13:41.45 Intake @15.8psia, vacuum from 10.0 to 13.0psi @13:13:41.96 SS.reconfigure :inhaling 0.43ml/s @13:13:42.02 SS.seek 25ml @13:14:01.34 Vacuum too high -- waiting up to 40 seconds @13:14:01.35 SS.reconfigure :exhale @13:14:01.39 SS.hold @13:14:21.62 Intake @15.6psia, vacuum from 10.0 to 13.0psi @13:14:21.64 SS.reconfigure :inhaling 0.38ml/s @13:14:21.68 SS.seek 25ml @13:14:22.78 Stroke completed -- waiting for pressure to equalize ... @13:14:22.79 SS.reconfigure :exhale @13:14:22.83 SS.hold @13:14:39.77 SS.reconfigure :default @13:14:39.81 SS.hold @13:14:39.92 Intake.select :close @13:14:40.06 ShallowSampler::Clogged in MAIN -- Filtering too Slowly after 325.0ml @13:14:40.07 SSV.dial :exhaust @13:14:40.81 Exhaust.select :open @13:14:40.92 SS.reconfigure :exhale @13:14:40.96 SS.seek 0ml @13:14:49.73 Exhaust.select :close @13:14:49.84 Sampled 325.0ml @13:14:49.85 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @13:14:49.86 Intake.select :close @13:14:49.91 CSV.dial :waste @13:14:49.96 CS.seek 0ml @13:14:50.06 CSV.dial :air @13:14:50.09 SSV.dial CBV,avoiding: :exhaust @13:14:50.13
CBV.dial SSV @13:14:50.84 CS.seek 10ml @13:14:52.02
CTV.dial :air @13:14:52.82 delay 15 seconds @13:15:07.84 SSV.dial :spit,avoiding: :exhaust @13:15:09.15 CTV.dial CSV,avoiding: :intake @13:15:09.19
SS.seek 0ml @13:15:09.30 SSV.dial CBV,avoiding: :exhaust @13:15:15.51 CBV.dial between SSV and waste2 @13:15:15.89 CTV.dial CSV,avoiding: :air @13:15:15.92
CSV.dial CTV @13:15:16.48 CS.reconfigure :pressurize @13:15:16.52 CS.seek 0ml @13:15:34.99 BubblePoint: Intake @39.4psia, Outlet @15.3psia @13:15:35.00 CBV.dial SSV @13:15:35.32 SS.reconfigure :default @13:15:35.37 SS.seek 10ml @13:15:40.97 delay 15 seconds @13:15:56.08 Evacuated: Intake @17.6psia, Outlet @9.3psia @13:15:56.09 SSV.dial :spit,avoiding: :exhaust @13:15:57.41 SS.reconfigure :exhale @13:15:57.45 SS.seek 0ml @13:16:02.77 SS.reconfigure :fast @13:16:02.81 SS.seek 5ml @13:16:05.58 SSV.dial CBV,avoiding: :exhaust @13:16:05.60
CBV.dial SSV @13:16:06.93 CTV.dial :air,avoiding: :intake @13:16:08.26 CSV.dial CTV @13:16:08.29
SS.seek 10ml @13:16:09.29 CSV.dial :air @13:16:09.73 CS.reconfigure :fast @13:16:09.77 CS.seek 0ml @13:16:10.77
SSV.dial :spit,avoiding: :exhaust @13:16:12.10 SS.reconfigure :exhale @13:16:12.14 SS.seek 0ml @13:16:17.33 CS.stop @13:16:17.85
SS.reconfigure :fast @13:16:17.89 SS.seek 5ml @13:16:20.54 SSV.dial CBV,avoiding: :exhaust @13:16:20.55
CBV.dial SSV @13:16:22.90 SSV.dial :spit,avoiding: :exhaust @13:16:24.21 SS.reconfigure :default @13:16:24.25 SS.seek 0ml @13:16:27.51 SS.stop @13:16:27.55 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @13:16:27.56 Intake.select :close @13:16:27.63 CSV.dial :waste @13:16:27.75 SSV.dial CBV,avoiding: :exhaust @13:16:27.76
CBV.dial SSV @13:16:28.39 CS.seek 0ml @13:16:28.46 CSV.dial :air @13:16:29.10
CTV.dial :air @13:16:29.11 delay 15 seconds @13:16:29.19 CS.seek 10ml @13:16:44.13
SSV.dial :spit,avoiding: :exhaust @13:16:45.46 CTV.dial CSV,avoiding: :intake @13:16:45.49
SS.reconfigure :exhale @13:16:45.58 SS.seek 0ml @13:16:45.98 SSV.dial CBV,avoiding: :exhaust @13:16:53.48 CBV.dial between SSV and waste2 @13:16:53.87 CTV.dial CSV,avoiding: :air @13:16:53.89
CSV.dial CTV @13:16:54.32 CS.reconfigure :pressurize @13:16:54.37 CS.seek 0ml @13:17:15.55 BubblePoint: Intake @38.3psia, Outlet @14.1psia @13:17:15.56 CBV.dial SSV @13:17:15.88 SS.reconfigure :default @13:17:15.93 SS.seek 10ml @13:17:21.61 delay 15 seconds @13:17:36.72 Evacuated: Intake @18.7psia, Outlet @11.5psia @13:17:36.73 SSV.dial :spit,avoiding: :exhaust @13:17:38.05 SS.reconfigure :exhale @13:17:38.09 SS.seek 0ml @13:17:43.34 SS.reconfigure :fast @13:17:43.38 SS.seek 5ml @13:17:46.38 SSV.dial CBV,avoiding: :exhaust @13:17:46.40
CBV.dial SSV @13:17:47.71 CTV.dial :air,avoiding: :intake @13:17:49.05 CSV.dial CTV @13:17:49.09
SS.seek 10ml @13:17:50.08 CSV.dial :air @13:17:50.54 CS.reconfigure :fast @13:17:50.58 CS.seek 0ml @13:17:51.76
SSV.dial :spit,avoiding: :exhaust @13:17:53.08 SS.reconfigure :exhale @13:17:53.12 SS.seek 0ml @13:17:55.80 CS.stop @13:17:58.18
SS.reconfigure :fast @13:17:58.22 SS.seek 5ml @13:18:01.06 SSV.dial CBV,avoiding: :exhaust @13:18:01.08
CBV.dial SSV @13:18:03.40 SSV.dial :spit,avoiding: :exhaust @13:18:04.72 SS.reconfigure :default @13:18:04.76 SS.seek 0ml @13:18:08.12 SS.stop @13:18:08.16 Lysing: Adding 2.0ml AqMeOH @13:18:08.17 CBV.dial between CSV and waste1,avoiding: :DBV @13:18:09.66 CTV.dial CSV @13:18:10.99 CSV.dial CSR @13:18:11.78 Pulling 1ml of CSR.AqMeOH CSR.select :AqMeOH @13:18:11.89 CS.reconfigure :default @13:18:11.93 CS.seek 1ml @13:18:15.36 delay 5 seconds @13:18:20.38 CSR.select :air @13:18:20.53 CS.seek 6ml @13:18:34.17 delay 2 seconds @13:18:36.19 CSV.dial :waste2 @13:18:36.62 CS.reconfigure :fast @13:18:36.66 CS.seek 0ml @13:18:51.05 CSV.dial CSR @13:18:51.54 Pulling 2ml of CSR.AqMeOH @13:18:51.55 CSR.select :AqMeOH @13:18:51.66 CS.reconfigure :default @13:18:51.70 CS.seek 2ml @13:18:57.86 delay 5 seconds @13:19:02.88 CSR.select :air @13:19:03.03 CS.seek 5ml @13:19:11.71 delay 2 seconds @13:19:13.72 CSV.dial CTV @13:19:14.15 CS.seek 1ml @13:19:25.59 Before lysing Collection Puck Heater at 18.6C/38.7psia @13:19:25.60 Heating Puck.from 4, :sh1 in Collection Clamp to 70C for 10:00 @13:19:25.62 CH.seek 70C,8:00 @13:21:21.27 CH.hold 10:00,70C @13:21:21.44 Begun lysing Collection Puck Heater at 68.1C/46.4psia @13:31:21.45 Ended lysing Collection Puck Heater at 70C/44.7psia @13:31:21.47 CS.seek 0ml @13:31:24.85 CTV.dial between spare and air,avoiding: :intake @13:31:25.88 CSV.dial :air @13:31:26.32 CS.seek 5ml @13:31:40.10 CSV.dial CTV @13:31:40.54 delay 2 seconds @13:31:42.55 CSV.dial :air @13:31:43.01 delay 2 seconds @13:31:45.02 CSV.dial CTV @13:31:45.47 delay 2 seconds @13:31:47.49 CSV.dial :air @13:31:47.94 delay 2 seconds @13:31:49.96 CS.seek 0ml @13:32:03.87 Puck @72.4psia after final lysis pressurization @13:32:03.88 Recovering Lysate into Collection syringe @13:32:03.89 CSV.dial CBV @13:32:04.89 CBV.dial CSV @13:32:05.25 CS.seek 5.001ml @13:32:18.82 delay 5 seconds @13:32:23.83 CTV.dial :air @13:32:24.36 CS.seek 7ml @13:32:30.32 delay 5 seconds @13:32:35.33 CSV.dial :air2 @13:32:35.78 CS.seek 10ml @13:32:44.32 CSV.dial between air and CTV,avoiding: CBV @13:32:45.46 Lysing Complete @13:32:45.53 Storing sh1 puck in Collection Clamp to tube 3 @13:32:45.68 SC.reconfigure :tight @13:32:45.72 SC.seek 3 @13:32:49.29 SC.configure :limp @13:32:49.48 Elbow.reconfigure :tight @13:32:49.52 Elbow.seek 3 @13:32:51.28 Forearm.reconfigure :tight @13:32:51.32 Forearm.seek 3 + 450 counts @13:32:52.32 SE.reconfigure :pick @13:32:52.37 SE.seek :beamBreak @13:32:54.01 SE.configure :default @13:32:54.15 Elbow.reconfigure :limp @13:32:54.19 Forearm.reconfigure :loose @13:32:54.23 Forearm.seek 3 @13:32:54.39 Elbow.hold @13:32:54.45 Elbow.reconfigure :tight @13:32:54.54 SE.seek 21.12 Pucks below Top Plate @13:32:55.07 Forearm.force -20 @13:32:55.57 Forearm.seek 3 + 313 counts @13:32:55.86 Forearm.reconfigure :tight @13:32:55.91 Forearm.seek 3 + 450 counts @13:32:56.10 SE.seek 22.12 Pucks below Top Plate @13:32:56.74 Forearm.reconfigure :loose @13:32:56.78 Forearm.seek :retract @13:32:57.74 Elbow.seek CC @13:32:59.42 Elbow.reconfigure :loose @13:32:59.50 Forearm.seek CC @13:33:00.51 Hand.close @13:33:01.09 CC.seek :open @13:33:05.80 Forearm.seek :retract @13:33:06.81 Elbow.reconfigure :tight @13:33:06.85 Elbow.seek 3 @13:33:08.53 Elbow.reconfigure :limp @13:33:08.57 Forearm.seek 3 @13:33:09.58 Elbow.hold @13:33:09.64 Elbow.reconfigure :tight @13:33:09.68 Hand.open @13:33:10.45 Forearm.force -20 @13:33:10.48 Forearm.seek 3 + 310 counts @13:33:10.76 Forearm.reconfigure :tight @13:33:10.80 Forearm.seek 3 + 450 counts @13:33:11.06 SE.seek :down @13:33:12.19
Forearm.reconfigure :loose @13:33:12.23 Forearm.seek :retract @13:33:12.27 SE.stop @13:33:13.27
Tranferring DA @13:33:13.28 Beginning samplePassC @13:33:13.29 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @13:33:13.46 Elbow.seek FlushPuck::Garage @13:33:15.30 Elbow.reconfigure :loose @13:33:15.39 Forearm.seek :garage @13:33:16.39 Hand.close @13:33:17.08 Forearm.seek :retract @13:33:18.05 Elbow.reconfigure :tight @13:33:18.09 Elbow.seek CC @13:33:21.02 Elbow.reconfigure :loose @13:33:21.11 Forearm.reconfigure :tight @13:33:21.15 Forearm.seek CC @13:33:22.37 CC.seek :closed @13:33:26.91 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.32 @13:33:26.97 Hand.open @13:33:27.65 Forearm.reconfigure :loose @13:33:27.69 Forearm.seek :retract @13:33:28.64 PSV.dial PSR @13:33:30.02 Pulling 1.5ml of PSR.flush @13:33:30.03 PSR.select :flush @13:33:30.14 PS.seek 1.5ml @13:33:46.33 delay 5 seconds @13:33:51.35 PSV.dial :waste1 @13:33:51.80 PS.seek 0ml @13:34:08.10 PSV.dial PSR @13:34:08.56 PSR.select :air @13:34:08.67 PS.seek 2.5ml @13:34:35.08 PRV.dial CBV,avoiding: :ab2 @13:34:35.10
CBV.dial PRV @13:34:36.76 PSV.dial :mixloop @13:34:37.80 CTV.dial :air,avoiding: :intake @13:34:37.81 PTV.dial :PRVmixing @13:34:39.11 PS.seek 0ml @13:35:05.48 PSV.dial :air @13:35:06.05 PS.seek 2.5ml @13:35:32.82 PSV.dial :mixloop @13:35:33.28 PS.seek 0ml @13:35:59.60 PSV.dial :air @13:36:00.06 PS.seek 2.5ml @13:36:26.48 PSV.dial :mixloop @13:36:26.94 PS.seek 0ml @13:36:53.29 Garaging FlushPuck in Collection Clamp @13:36:53.36 Elbow.reconfigure :tight @13:36:53.40 Elbow.seek CC @13:36:53.80 Elbow.reconfigure :loose @13:36:53.89 Forearm.seek CC @13:36:54.85 Hand.close @13:36:55.47 CC.seek :open @13:37:00.08 Forearm.seek :retract @13:37:01.03 Elbow.reconfigure :tight @13:37:01.07 Elbow.seek :garage @13:37:03.96 Elbow.reconfigure :loose @13:37:04.04 Forearm.reconfigure :tight @13:37:04.08 Forearm.seek :garage @13:37:05.12 Hand.open @13:37:05.77 Forearm.reconfigure :loose @13:37:05.82 Forearm.seek :retract @13:37:06.78 PSV.dial :mixloop @13:37:06.79 PTV.dial :PRVmixing @13:37:06.80 PRV.dial CBV @13:37:06.83
CBV.dial PRV @13:37:06.84 CTV.dial :air,avoiding: :intake @13:37:06.86 Loading lyfil puck from tube 4 into Collection Clamp @13:37:07.07 SC.reconfigure :tight @13:37:07.11 SC.seek 4 @13:37:10.71 SC.configure :limp @13:37:10.90 Elbow.reconfigure :tight @13:37:10.94 Elbow.seek 4 @13:37:12.84 Forearm.reconfigure :tight @13:37:12.89 Forearm.seek 4 + 450 counts @13:37:13.96 SE.reconfigure :pick @13:37:14.01 SE.seek :beamBreak @13:37:16.52 SE.configure :default @13:37:16.67 Elbow.seek 4 @13:37:16.84 Forearm.seek 4 + 300 counts @13:37:17.02 Forearm.reconfigure :loose @13:37:17.07 Hand.close @13:37:17.73 SE.seek :down @13:37:17.92
Forearm.seek :retract @13:37:18.87 Elbow.seek CC @13:37:19.93 SE.stop @13:37:20.53
Elbow.reconfigure :loose @13:37:20.62 Forearm.reconfigure :tight @13:37:20.66 Forearm.seek CC @13:37:21.91 CC.seek :closed @13:37:26.42 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.14 @13:37:26.43 Hand.open @13:37:27.15 Forearm.reconfigure :loose @13:37:27.19 Forearm.seek :retract @13:37:28.15 CTV.dial CSV,avoiding: :intake @13:37:29.51 CSV.dial CTV @13:37:29.82 PS.seek 2.5ml @13:37:56.19 CS.seek 4.5ml @13:38:11.12 delay 8 seconds @13:38:19.14 PSV.dial :air @13:38:19.60 delay 2 seconds @13:38:21.62 PSV.dial :waste2 @13:38:22.34 PS.seek 0ml @13:38:48.91 CBV.dial between PRV and waste1 @13:38:49.69 CSV.dial :air @13:38:50.16 CS.seek 10ml @13:39:05.03 CSV.dial CTV @13:39:05.47 CS.seek 0ml @13:39:31.84 CBV.dial :waste1 @13:39:32.14 delay 5 seconds @13:39:37.16 CTV.dial :air,avoiding: :intake @13:39:38.54 Storing lyfil puck in Collection Clamp to tube 3 @13:39:38.70 SC.reconfigure :tight @13:39:38.74 SC.seek 3 @13:39:42.35 SC.configure :limp @13:39:42.54 Elbow.reconfigure :tight @13:39:42.58 Elbow.seek 3 @13:39:44.38 Forearm.reconfigure :tight @13:39:44.43 Forearm.seek 3 + 450 counts @13:39:45.43 SE.reconfigure :pick @13:39:45.48 SE.seek :beamBreak @13:39:47.22 SE.configure :default @13:39:47.37 Elbow.reconfigure :limp @13:39:47.42 Forearm.reconfigure :loose @13:39:47.46 Forearm.seek 3 @13:39:47.69 Elbow.hold @13:39:47.75 Elbow.reconfigure :tight @13:39:47.84 SE.seek 22.12 Pucks below Top Plate @13:39:48.42 Forearm.force -20 @13:39:48.45 Forearm.seek 3 + 302 counts @13:39:48.73 Forearm.reconfigure :tight @13:39:48.77 Forearm.seek 3 + 450 counts @13:39:48.98 SE.seek 23.12 Pucks below Top Plate @13:39:49.51 Forearm.reconfigure :loose @13:39:49.55 Forearm.seek :retract @13:39:50.48 Elbow.seek CC @13:39:52.17 Elbow.reconfigure :loose @13:39:52.26 Forearm.seek CC @13:39:53.23 Hand.close @13:39:53.84 CC.seek :open @13:39:58.56 Forearm.seek :retract @13:39:59.53 Elbow.reconfigure :tight @13:39:59.57 Elbow.seek 3 @13:40:01.24 Elbow.reconfigure :limp @13:40:01.28 Forearm.seek 3 @13:40:02.30 Elbow.hold @13:40:02.36 Elbow.reconfigure :tight @13:40:02.41 Hand.open @13:40:03.07 Forearm.force -20 @13:40:03.10 Forearm.seek 3 + 309 counts @13:40:03.38 Forearm.reconfigure :tight @13:40:03.42 Forearm.seek 3 + 450 counts @13:40:03.69 SE.seek :down @13:40:04.82
Forearm.reconfigure :loose @13:40:04.87 Forearm.seek :retract @13:40:04.89 SE.stop @13:40:05.82
PSV.dial :air @13:40:06.58 PS.seek 2.5ml @13:40:32.97 delay 1 second @13:40:33.99 PSV.dial :mixloop @13:40:34.43 delay 1 second @13:40:35.44 PSV.dial :air @13:40:36.02 delay 1 second @13:40:37.03 PSV.dial :mixloop @13:40:37.47 delay 1 second @13:40:38.49 PSV.dial :air @13:40:38.95 delay 1 second @13:40:39.97 PS.seek 0ml @13:41:06.32 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @13:41:06.48 Elbow.seek FlushPuck::Garage @13:41:08.29 Elbow.reconfigure :loose @13:41:08.38 Forearm.seek :garage @13:41:09.35 Hand.close @13:41:10.57 Forearm.seek :retract @13:41:11.53 Elbow.reconfigure :tight @13:41:11.57 Elbow.seek PC @13:41:12.80 Elbow.reconfigure :loose @13:41:12.88 Forearm.reconfigure :tight @13:41:12.93 Forearm.seek PC @13:41:14.09 PC.seek :closed @13:41:18.73 Processing Clamp onSpring/offSpring for 16/26 samples, on/off torque 1.59 @13:41:18.81 Hand.open @13:41:19.52 Forearm.reconfigure :loose @13:41:19.56 Forearm.seek :retract @13:41:20.52 PRV.dial :air @13:41:20.97 PSV.dial :mixloop @13:41:21.50 PS.seek 0.3ml @13:41:25.44 PSV.dial PSR @13:41:26.53 Pulling 0.4ml of PSR.air PSR.select :air @13:41:26.56 PS.seek 0.7ml @13:41:31.47 delay 3 seconds @13:41:34.49 PSR.select :air @13:41:34.51 PS.seek 2.5ml @13:41:53.91 delay 3 seconds @13:41:56.93 PSV.dial :mixloop @13:41:57.96 PTV.dial :mixing @13:41:58.71 PBV.dial :waste2 @13:41:59.65 PS.seek 0ml @13:42:26.07 Garaging FlushPuck in Processing Clamp @13:42:26.13 Elbow.reconfigure :tight @13:42:26.18 Elbow.seek PC @13:42:26.28 Elbow.reconfigure :loose @13:42:26.36 Forearm.seek PC @13:42:27.30 Hand.close @13:42:27.96 PC.seek :open @13:42:32.67 Forearm.seek :retract @13:42:33.63 Elbow.reconfigure :tight @13:42:33.67 Elbow.seek :garage @13:42:35.03 Elbow.reconfigure :loose @13:42:35.12 Forearm.reconfigure :tight @13:42:35.16 Forearm.seek :garage @13:42:36.19 Hand.open @13:42:36.89 Forearm.reconfigure :loose @13:42:36.93 Forearm.seek :retract @13:42:37.92 Loading da puck from tube 4 into Processing Clamp @13:42:38.14 SC.reconfigure :tight @13:42:38.18 SC.seek 4 @13:42:41.76 SC.configure :limp @13:42:41.95 Elbow.reconfigure :tight @13:42:41.99 Elbow.seek 4 @13:42:43.83 Forearm.reconfigure :tight @13:42:43.87 Forearm.seek 4 + 450 counts @13:42:44.89 SE.reconfigure :pick @13:42:44.94 SE.seek :beamBreak @13:42:47.45 SE.configure :default @13:42:47.59 Elbow.seek 4 @13:42:47.75 Forearm.seek 4 + 300 counts @13:42:47.96 Forearm.reconfigure :loose @13:42:48.00 Hand.close @13:42:48.60 SE.seek :down @13:42:48.78
Forearm.seek :retract @13:42:49.71 Elbow.seek PC @13:42:50.83 SE.stop @13:42:52.23
Elbow.reconfigure :loose @13:42:52.32 Forearm.reconfigure :tight @13:42:52.36 Forearm.seek PC @13:42:54.03 PC.seek :closed @13:42:58.67 Processing Clamp onSpring/offSpring for 16/26 samples, on/off torque 1.58 @13:42:58.68 Hand.open @13:42:59.39 Forearm.reconfigure :loose @13:42:59.43 Forearm.seek :retract @13:43:00.39 Heating Puck.from 4, :da in Processing Clamp to 28C for 2:00:00 @13:43:00.41 PH.seek 28C,5:00 @13:43:33.98 PH.hold 2:00:00,28C @13:43:34.02 delay 1:30 @13:45:04.06 PSR.select :air @13:45:04.08 PSV.dial PSR @13:45:05.16 Pulling 0.5ml of PSR.wash @13:45:05.17 PSR.select :wash @13:45:05.29 PS.seek 0.5ml @13:45:11.41 delay 5 seconds @13:45:16.42 PSR.select :air @13:45:16.53 PS.seek 2ml @13:45:32.68 PTV.dial :puck @13:45:33.41 PSV.dial PTV @13:45:34.16 PBV.dial :waste1 @13:45:35.54 PS.seek 1.5ml @13:45:41.50 delay 3 seconds @13:45:44.51 PBV.dial between PSV and waste1 @13:45:44.87 PS.seek 0ml @13:46:01.03 delay 5:00 @13:51:01.05 recoverPSRreagent08 @13:51:01.06 PTV.dial :puck @13:51:01.07 PBV.dial :waste1 @13:51:01.41 delay 30 seconds @13:51:31.44 PBV.dial between PSV and waste1 @13:51:31.81 PSR.select :air @13:51:31.82 PSV.dial PSR @13:51:32.57 PS.reconfigure :fast @13:51:32.62 PS.seek 2.5ml @13:51:56.05 PSV.dial PTV @13:51:56.79 delay 5 seconds @13:52:01.81 PSV.dial PSR @13:52:02.68 delay 5 seconds @13:52:07.70 PSV.dial PTV @13:52:08.43 delay 5 seconds @13:52:13.45 PSV.dial :air @13:52:14.20 PS.seek 0ml @13:52:37.18 PTV.dial :PRVmixing,via: PRV @13:52:38.49 PSV.dial :mixloop @13:52:38.94 PRV.dial :ab1 @13:52:39.67 PS.reconfigure :slow @13:52:39.71 PS.seek 0.15ml @13:52:43.30 delay 2 seconds @13:52:45.32 CBV.dial PRV @13:52:45.34
PRV.dial CBV @13:52:45.90 PS.seek 0.35ml @13:52:50.43 delay 2 seconds @13:52:52.44 CBV.dial between PRV and PSV @13:52:52.77 PRV.dial :air @13:52:53.22 PS.seek 0.9ml @13:53:04.99 PRV.dial :waste2 @13:53:05.72 PS.seek 0.5ml @13:53:14.30 addPMLReagent08 @13:53:14.31 PSV.dial :air @13:53:14.78 PS.reconfigure :default @13:53:14.82 PS.seek 2ml @13:53:30.92 PSV.dial :PML @13:53:31.38 PTV.dial :mixing @13:53:32.11 PBV.dial :waste1 @13:53:32.53 PS.seek 1.85ml @13:53:34.93 PBV.dial between PSV and waste1 @13:53:35.29 PS.seek 0ml @13:53:55.08 delay 2 seconds @13:53:57.10 Beginning samplePassC @13:53:57.11 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @13:53:57.27 Elbow.reconfigure :tight @13:53:57.31 Elbow.seek FlushPuck::Garage @13:53:59.12 Elbow.reconfigure :loose @13:53:59.23 Forearm.seek :garage @13:54:00.22 Hand.close @13:54:00.94 Forearm.seek :retract @13:54:01.91 Elbow.reconfigure :tight @13:54:01.95 Elbow.seek CC @13:54:04.82 Elbow.reconfigure :loose @13:54:04.90 Forearm.reconfigure :tight @13:54:04.95 Forearm.seek CC @13:54:06.13 CC.seek :closed @13:54:10.68 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.32 @13:54:10.75 Hand.open @13:54:11.39 Forearm.reconfigure :loose @13:54:11.43 Forearm.seek :retract @13:54:12.40 PSV.dial PSR @13:54:13.49 Pulling 1.5ml of PSR.flush @13:54:13.50 PSR.select :flush @13:54:13.62 PS.seek 1.5ml @13:54:29.83 delay 5 seconds @13:54:34.85 PSV.dial :waste1 @13:54:35.32 PS.seek 0ml @13:54:51.49 PSV.dial PSR @13:54:51.96 PSR.select :air @13:54:52.07 PS.seek 2.5ml @13:55:18.43 PRV.dial CBV,avoiding: :ab2 @13:55:18.45
CBV.dial PRV @13:55:20.07 PSV.dial :mixloop @13:55:21.10 CTV.dial :air,avoiding: :intake @13:55:21.11 PTV.dial :PRVmixing @13:55:21.85 PS.seek 0ml @13:55:48.20 PSV.dial :air @13:55:48.66 PS.seek 2.5ml @13:56:14.95 PSV.dial :mixloop @13:56:15.41 PS.seek 0ml @13:56:42.01 PSV.dial :air @13:56:42.48 PS.seek 2.5ml @13:57:08.86 PSV.dial :mixloop @13:57:09.32 PS.seek 0ml @13:57:35.74 Garaging FlushPuck in Collection Clamp @13:57:35.80 Elbow.reconfigure :tight @13:57:35.84 Elbow.seek CC @13:57:36.16 Elbow.reconfigure :loose @13:57:36.24 Forearm.seek CC @13:57:37.21 Hand.close @13:57:37.82 CC.seek :open @13:57:42.46 Forearm.seek :retract @13:57:43.42 Elbow.reconfigure :tight @13:57:43.46 Elbow.seek :garage @13:57:46.34 Elbow.reconfigure :loose @13:57:46.43 Forearm.reconfigure :tight @13:57:46.47 Forearm.seek :garage @13:57:47.51 Hand.open @13:57:48.13 Forearm.reconfigure :loose @13:57:48.17 Forearm.seek :retract @13:57:49.17 delayUntil "14:01:57.09" @13:57:49.19 Whole Cell Archive sampling 1000ml @13:57:49.21 Loading wcr puck from tube 4 into Collection Clamp @13:57:49.43 SC.reconfigure :tight @13:57:49.47 SC.seek 4 @13:57:49.58 SC.configure :limp @13:57:49.79 Elbow.reconfigure :tight @13:57:49.84 Elbow.seek 4 @13:57:51.75 Forearm.reconfigure :tight @13:57:51.79 Forearm.seek 4 + 450 counts @13:57:53.28 SE.reconfigure :pick @13:57:53.35 SE.seek :beamBreak @13:57:56.01 SE.configure :default @13:57:56.16 Elbow.seek 4 @13:57:56.33 Forearm.seek 4 + 300 counts @13:57:56.56 Forearm.reconfigure :loose @13:57:56.60 Hand.close @13:57:57.21 SE.seek :down @13:57:57.40 Forearm.seek :retract @13:57:58.36 Elbow.seek CC @13:57:59.53 SE.stop @13:58:00.00 Elbow.reconfigure :loose @13:58:00.10 Forearm.reconfigure :tight @13:58:00.15 Forearm.seek CC @13:58:01.42 CC.seek :closed @13:58:05.93 Collection Clamp onSpring/offSpring for 13/27 samples, on/off torque 2.18 @13:58:05.95 Hand.open @13:58:06.63 Forearm.reconfigure :loose @13:58:06.67 Forearm.seek :retract @13:58:07.64 collectSample 1000ml w/bubble point of 35psi @13:58:07.65 Priming 7ml @13:58:07.66 Intake.select :close @13:58:07.67 Exhaust.select :close @13:58:07.68 SS.hold @13:58:07.87 Calibrating pressure sensors @13:58:07.88 CTV.dial :air @13:58:07.90 SSV.dial :spit,avoiding: :exhaust @13:58:07.91 SS.reconfigure :fast @13:58:07.96 SS.seek 5ml @13:58:10.54 SSV.dial CBV @13:58:10.59 CBV.dial SSV @13:58:11.89 delay 3.8 seconds @13:58:15.81 SSV.dial :spit @13:58:17.13 SS.seek 0ml @13:58:19.84 SSV.dial CBV @13:58:21.17 CTV.dial :intake @13:58:21.93 SSV.dial CBV @13:58:21.95 CBV.dial SSV @13:58:21.97 Allowing 40s for 7.0ml of remaining 7.0ml @13:58:21.99 Intake.select :open @13:58:24.14 Intake @15.1psia, vacuum from 9.6 to 12.6psi @13:58:24.16 SS.reconfigure :inhaling 2.20ml/s @13:58:24.20 SS.seek 7ml @13:58:30.32 Stroke completed -- waiting for pressure to equalize ... @13:58:30.33 SS.reconfigure :exhale @13:58:30.38 SS.hold @13:58:43.00 Intake.select :close @13:58:43.15 SSV.dial :exhaust @13:58:43.90 Exhaust.select :open @13:58:44.01 SS.seek 0ml @13:58:48.29 Exhaust.select :close @13:58:48.40 Sampling 1000.0ml @13:58:48.41 Intake.select :close @13:58:48.42 Exhaust.select :close @13:58:48.43 SS.reconfigure :default @13:58:48.47 SS.hold @13:58:48.66 CTV.dial :intake @13:58:49.16 SSV.dial CBV @13:58:49.18 CBV.dial SSV @13:58:49.93 Allowing 130s for 25.0ml of remaining 1000.0ml @13:58:49.95 Intake.select :open @13:58:52.10 Intake @15.5psia, vacuum from 10.0 to 13.0psi @13:58:52.12 SS.reconfigure :inhaling 2.20ml/s @13:58:52.16 SS.seek 25ml @13:58:58.63 Vacuum too high -- waiting up to 40 seconds @13:58:58.64 SS.reconfigure :exhale @13:58:58.68 SS.hold @13:59:03.17 Intake @14.4psia, vacuum from 8.9 to 11.9psi @13:59:03.19 SS.reconfigure :inhaling 1.98ml/s @13:59:03.23 SS.seek 25ml @13:59:09.50 Vacuum too high -- waiting up to 40 seconds @13:59:09.51 SS.reconfigure :exhale @13:59:09.56 SS.hold @13:59:17.28 Intake @15.6psia, vacuum from 10.0 to 13.0psi @13:59:17.30 SS.reconfigure :inhaling 1.79ml/s @13:59:17.34 SS.seek 25ml @13:59:21.51 Stroke completed -- waiting for pressure to equalize ... @13:59:21.52 SS.reconfigure :exhale @13:59:21.56 SS.hold @13:59:27.22 Intake.select :close @13:59:27.37 SSV.dial :exhaust @13:59:28.10 Exhaust.select :open @13:59:28.21 SS.seek 0ml @13:59:37.05 Exhaust.select :close @13:59:37.16 SSV.dial CBV @13:59:37.18 CBV.dial SSV @13:59:37.93 Allowing 130s for 25.0ml of remaining 975.0ml @13:59:37.94 Intake.select :open @13:59:40.10 Intake @15.9psia, vacuum from 10.0 to 13.0psi @13:59:40.12 SS.reconfigure :inhaling 1.79ml/s @13:59:40.16 SS.seek 25ml @13:59:48.69 Vacuum too high -- waiting up to 40 seconds @13:59:48.70 SS.reconfigure :exhale @13:59:48.74 SS.hold @13:59:52.48 Intake @14.6psia, vacuum from 9.1 to 12.1psi @13:59:52.50 SS.reconfigure :inhaling 1.61ml/s @13:59:52.54 SS.seek 25ml @14:00:01.91 Vacuum too high -- waiting up to 40 seconds @14:00:01.92 SS.reconfigure :exhale @14:00:01.96 SS.hold @14:00:09.53 Intake @15.1psia, vacuum from 9.6 to 12.6psi @14:00:09.54 SS.reconfigure :inhaling 1.45ml/s @14:00:09.59 SS.seek 25ml @14:00:11.19 Stroke completed -- waiting for pressure to equalize ... @14:00:11.21 SS.reconfigure :exhale @14:00:11.75 SS.hold @14:00:14.61 Intake.select :close @14:00:14.75 SSV.dial :exhaust @14:00:15.49 Exhaust.select :open @14:00:15.60 SS.seek 0ml @14:00:24.57 Exhaust.select :close @14:00:24.68 SSV.dial CBV @14:00:24.71 CBV.dial SSV @14:00:25.45 Allowing 130s for 25.0ml of remaining 950.0ml @14:00:25.46 Intake.select :open @14:00:27.61 Intake @15.7psia, vacuum from 10.0 to 13.0psi @14:00:27.63 SS.reconfigure :inhaling 1.45ml/s @14:00:27.67 SS.seek 25ml @14:00:38.25 Vacuum too high -- waiting up to 40 seconds @14:00:38.26 SS.reconfigure :exhale @14:00:38.30 SS.hold @14:00:42.01 Intake @14.3psia, vacuum from 8.8 to 11.8psi @14:00:42.02 SS.reconfigure :inhaling 1.30ml/s @14:00:42.07 SS.seek 25ml @14:00:52.91 Stroke completed -- waiting for pressure to equalize ... @14:00:52.92 SS.reconfigure :exhale @14:00:52.97 SS.hold @14:01:03.99 Intake.select :close @14:01:04.13 SSV.dial :exhaust @14:01:04.87 Exhaust.select :open @14:01:04.98 SS.seek 0ml @14:01:13.82 Exhaust.select :close @14:01:13.92 SSV.dial CBV @14:01:13.98 CBV.dial SSV @14:01:14.68 Allowing 130s for 25.0ml of remaining 925.0ml @14:01:14.69 Intake.select :open @14:01:16.85 Intake @15.8psia, vacuum from 10.0 to 13.0psi @14:01:16.86 SS.reconfigure :inhaling 1.30ml/s @14:01:16.91 SS.seek 25ml @14:01:28.03 Vacuum too high -- waiting up to 40 seconds @14:01:28.04 SS.reconfigure :exhale @14:01:28.08 SS.hold @14:01:31.66 Intake @14.4psia, vacuum from 8.9 to 11.9psi @14:01:31.68 SS.reconfigure :inhaling 1.17ml/s @14:01:31.72 SS.seek 25ml @14:01:44.41 Stroke completed -- waiting for pressure to equalize ... @14:01:44.42 SS.reconfigure :exhale @14:01:44.47 SS.hold @14:01:55.59 Intake.select :close @14:01:56.18 SSV.dial :exhaust @14:01:56.93 Exhaust.select :open @14:01:57.04 SS.seek 0ml @14:01:57.10 recoverPRVreagent08 @14:01:57.11 PTV.dial :mixing @14:01:57.85 PBV.dial :waste1 @14:01:58.22 delay 30 seconds @14:02:06.12 Exhaust.select :close @14:02:06.23 SSV.dial CBV @14:02:06.25 CBV.dial SSV @14:02:07.01 Allowing 130s for 25.0ml of remaining 900.0ml @14:02:07.02 Intake.select :open @14:02:09.18 Intake @15.6psia, vacuum from 10.0 to 13.0psi @14:02:09.19 SS.reconfigure :inhaling 1.17ml/s @14:02:09.24 SS.seek 25ml @14:02:24.68 Vacuum too high -- waiting up to 40 seconds @14:02:24.70 SS.reconfigure :exhale @14:02:24.74 SS.hold @14:02:28.28 PBV.dial between PSV and waste1 @14:02:28.64 PSV.dial :air @14:02:29.11 PS.reconfigure :fast @14:02:29.15 PS.seek 2.5ml @14:02:30.41 Intake @14.7psia, vacuum from 9.2 to 12.2psi @14:02:30.43 SS.reconfigure :inhaling 1.06ml/s @14:02:30.47 SS.seek 25ml @14:02:40.02 Stroke completed -- waiting for pressure to equalize ... @14:02:40.03 SS.reconfigure :exhale @14:02:40.07 SS.hold @14:02:51.21 Intake.select :close @14:02:51.35 SSV.dial :exhaust @14:02:52.11 Exhaust.select :open @14:02:52.22 SS.seek 0ml @14:02:52.95 PSV.dial :mixloop @14:02:53.42 delay 2 seconds @14:02:55.43 PSV.dial :air @14:02:55.88 delay 2 seconds @14:02:57.90 PSV.dial :mixloop @14:02:58.37 delay 2 seconds @14:03:00.39 PSV.dial :air @14:03:00.84 PS.seek 0ml @14:03:01.02 Exhaust.select :close @14:03:01.13 SSV.dial CBV @14:03:01.15 CBV.dial SSV @14:03:01.93 Allowing 130s for 25.0ml of remaining 875.0ml @14:03:01.94 Intake.select :open @14:03:04.09 Intake @15.7psia, vacuum from 10.0 to 13.0psi @14:03:04.11 SS.reconfigure :inhaling 1.06ml/s @14:03:04.15 SS.seek 25ml @14:03:19.15 Vacuum too high -- waiting up to 40 seconds @14:03:19.16 SS.reconfigure :exhale @14:03:19.21 SS.hold @14:03:24.36 washCycleDA #1 of 2 @14:03:24.37 PSR.select :air @14:03:24.38 PSV.dial PSR @14:03:24.82 Intake @14.9psia, vacuum from 9.4 to 12.4psi @14:03:24.84 SS.reconfigure :inhaling 0.95ml/s @14:03:24.88 SS.seek 25ml @14:03:25.75 Pulling 0.5ml of PSR.wash @14:03:25.76 PSR.select :wash @14:03:25.87 PS.reconfigure :default @14:03:25.91 PS.seek 0.5ml @14:03:31.85 delay 5 seconds @14:03:36.87 PSR.select :air @14:03:36.98 PS.seek 2ml @14:03:37.86 Stroke completed -- waiting for pressure to equalize ... @14:03:37.87 SS.reconfigure :exhale @14:03:38.39 SS.hold @14:03:49.38 Intake.select :close @14:03:49.52 SSV.dial :exhaust @14:03:50.28 Exhaust.select :open @14:03:50.39 SS.seek 0ml @14:03:53.07 PTV.dial :puck @14:03:53.80 PSV.dial PTV @14:03:54.54 PBV.dial :waste1 @14:03:54.97 PS.seek 1.5ml @14:03:59.60 Exhaust.select :close @14:03:59.70 SSV.dial CBV @14:03:59.74 CBV.dial SSV @14:04:00.47 Allowing 130s for 25.0ml of remaining 850.0ml @14:04:00.49 Intake.select :open @14:04:00.88 delay 3 seconds @14:04:02.64 Intake @15.6psia, vacuum from 10.0 to 13.0psi @14:04:02.66 SS.reconfigure :inhaling 0.95ml/s @14:04:02.70 SS.seek 25ml @14:04:03.90 PBV.dial between PSV and waste1 @14:04:04.28 PS.seek 0ml @14:04:20.40 Vacuum too high -- waiting up to 40 seconds @14:04:20.41 SS.reconfigure :exhale @14:04:20.46 SS.hold @14:04:20.50 delay 2:00 @14:04:27.00 Intake @14.9psia, vacuum from 9.4 to 12.4psi @14:04:27.02 SS.reconfigure :inhaling 0.87ml/s @14:04:27.06 SS.seek 25ml @14:04:38.94 Stroke completed -- waiting for pressure to equalize ... @14:04:38.95 SS.reconfigure :exhale @14:04:38.99 SS.hold @14:04:51.63 Intake.select :close @14:04:51.77 SSV.dial :exhaust @14:04:52.52 Exhaust.select :open @14:04:52.63 SS.seek 0ml @14:05:01.44 Exhaust.select :close @14:05:01.54 SSV.dial CBV @14:05:01.60 CBV.dial SSV @14:05:02.30 Allowing 130s for 25.0ml of remaining 825.0ml @14:05:02.31 Intake.select :open @14:05:04.47 Intake @15.7psia, vacuum from 10.0 to 13.0psi @14:05:04.48 SS.reconfigure :inhaling 0.87ml/s @14:05:04.53 SS.seek 25ml @14:05:21.84 Vacuum too high -- waiting up to 40 seconds @14:05:21.85 SS.reconfigure :exhale @14:05:21.89 SS.hold @14:05:28.39 Intake @15.1psia, vacuum from 9.6 to 12.6psi @14:05:28.41 SS.reconfigure :inhaling 0.78ml/s @14:05:28.45 SS.seek 25ml @14:05:44.45 Stroke completed -- waiting for pressure to equalize ... @14:05:44.46 SS.reconfigure :exhale @14:05:44.51 SS.hold @14:05:58.77 Intake.select :close @14:05:58.91 SSV.dial :exhaust @14:05:59.66 Exhaust.select :open @14:05:59.77 SS.seek 0ml @14:06:08.84 Exhaust.select :close @14:06:08.95 SSV.dial CBV @14:06:08.97 CBV.dial SSV @14:06:09.72 Allowing 130s for 25.0ml of remaining 800.0ml @14:06:09.73 Intake.select :open @14:06:11.89 Intake @15.6psia, vacuum from 10.0 to 13.0psi @14:06:11.91 SS.reconfigure :inhaling 0.78ml/s @14:06:11.95 SS.seek 25ml @14:06:20.53 recoverPSRreagent08 @14:06:20.54 PTV.dial :puck @14:06:20.55 PBV.dial :waste1 @14:06:20.91 delay 30 seconds @14:06:31.59 Vacuum too high -- waiting up to 40 seconds @14:06:31.60 SS.reconfigure :exhale @14:06:31.65 SS.hold @14:06:39.11 Intake @15.1psia, vacuum from 9.6 to 12.6psi @14:06:39.13 SS.reconfigure :inhaling 0.70ml/s @14:06:39.17 SS.seek 25ml @14:06:50.94 PBV.dial between PSV and waste1 @14:06:51.30 PSR.select :air @14:06:51.32 PSV.dial PSR @14:06:52.09 PS.reconfigure :fast @14:06:52.13 PS.seek 2.5ml @14:06:55.45 Stroke completed -- waiting for pressure to equalize ... @14:06:55.46 SS.reconfigure :exhale @14:06:55.51 SS.hold @14:07:13.85 Intake.select :close @14:07:13.99 SSV.dial :exhaust @14:07:14.73 Exhaust.select :open @14:07:14.84 SS.seek 0ml @14:07:15.85 PSV.dial PTV @14:07:16.61 delay 5 seconds @14:07:21.63 PSV.dial PSR @14:07:22.50 delay 5 seconds @14:07:23.89 Exhaust.select :close @14:07:24.00 SSV.dial CBV @14:07:24.03 CBV.dial SSV @14:07:24.77 Allowing 130s for 25.0ml of remaining 775.0ml @14:07:24.78 Intake.select :open @14:07:27.40 Intake @15.7psia, vacuum from 10.0 to 13.0psi @14:07:27.41 SS.reconfigure :inhaling 0.70ml/s @14:07:27.46 SS.seek 25ml @14:07:27.52 PSV.dial PTV @14:07:28.26 delay 5 seconds @14:07:33.27 PSV.dial :air @14:07:34.04 PS.seek 0ml @14:07:46.33 Vacuum too high -- waiting up to 40 seconds @14:07:46.35 SS.reconfigure :exhale @14:07:46.39 SS.hold @14:07:54.94 Intake @15.3psia, vacuum from 9.8 to 12.8psi @14:07:54.96 SS.reconfigure :inhaling 0.63ml/s @14:07:55.00 SS.seek 25ml @14:07:57.29 washCycleDA #2 of 2 @14:07:57.30 PSR.select :air @14:07:57.32 PSV.dial PSR @14:07:58.68 Pulling 0.5ml of PSR.wash @14:07:58.69 PSR.select :wash @14:07:58.80 PS.reconfigure :default @14:07:58.84 PS.seek 0.5ml @14:08:04.72 delay 5 seconds @14:08:09.74 PSR.select :air @14:08:09.86 PS.seek 2ml @14:08:16.56 Stroke completed -- waiting for pressure to equalize ... @14:08:16.57 SS.reconfigure :exhale @14:08:16.61 SS.hold @14:08:26.03 PTV.dial :puck @14:08:26.04 PSV.dial PTV @14:08:26.78 PBV.dial :waste1 @14:08:27.21 PS.seek 1.5ml @14:08:33.16 delay 3 seconds @14:08:36.17 PBV.dial between PSV and waste1 @14:08:36.56 PS.seek 0ml @14:08:36.99 Intake.select :close @14:08:37.14 SSV.dial :exhaust @14:08:37.88 Exhaust.select :open @14:08:37.99 SS.seek 0ml @14:08:46.82 Exhaust.select :close @14:08:46.93 SSV.dial CBV @14:08:46.95 CBV.dial SSV @14:08:47.70 Allowing 130s for 25.0ml of remaining 750.0ml @14:08:47.72 Intake.select :open @14:08:49.87 Intake @15.7psia, vacuum from 10.0 to 13.0psi @14:08:49.89 SS.reconfigure :inhaling 0.63ml/s @14:08:49.93 SS.seek 25ml @14:08:52.66 delay 2:00 @14:09:06.71 Vacuum too high -- waiting up to 40 seconds @14:09:06.72 SS.reconfigure :exhale @14:09:06.76 SS.hold @14:09:14.22 Intake @15.3psia, vacuum from 9.8 to 12.8psi @14:09:14.24 SS.reconfigure :inhaling 0.57ml/s @14:09:14.28 SS.seek 25ml @14:09:35.80 Vacuum too high -- waiting up to 40 seconds @14:09:35.81 SS.reconfigure :exhale @14:09:35.86 SS.hold @14:09:48.87 Intake @15.5psia, vacuum from 10.0 to 13.0psi @14:09:48.89 SS.reconfigure :inhaling 0.51ml/s @14:09:48.93 SS.seek 25ml @14:09:56.67 Stroke completed -- waiting for pressure to equalize ... @14:09:56.68 SS.reconfigure :exhale @14:09:56.72 SS.hold @14:10:11.07 Intake.select :close @14:10:11.22 SSV.dial :exhaust @14:10:11.96 Exhaust.select :open @14:10:12.07 SS.seek 0ml @14:10:20.81 Exhaust.select :close @14:10:20.91 SSV.dial CBV @14:10:20.94 CBV.dial SSV @14:10:21.68 Allowing 130s for 25.0ml of remaining 725.0ml @14:10:21.70 Intake.select :open @14:10:23.85 Intake @15.9psia, vacuum from 10.0 to 13.0psi @14:10:23.87 SS.reconfigure :inhaling 0.51ml/s @14:10:23.91 SS.seek 25ml @14:10:45.46 Vacuum too high -- waiting up to 40 seconds @14:10:45.47 SS.reconfigure :exhale @14:10:45.52 SS.hold @14:10:52.68 recoverPSRreagent08 @14:10:52.69 PTV.dial :puck @14:10:52.71 PBV.dial :waste1 @14:10:53.08 delay 30 seconds @14:10:54.06 Intake @15.4psia, vacuum from 9.9 to 12.9psi @14:10:54.08 SS.reconfigure :inhaling 0.47ml/s @14:10:54.12 SS.seek 25ml @14:11:23.10 PBV.dial between PSV and waste1 @14:11:23.45 PSR.select :air @14:11:23.47 PSV.dial PSR @14:11:24.25 Vacuum too high -- waiting up to 40 seconds @14:11:24.26 SS.reconfigure :exhale @14:11:24.33 SS.hold @14:11:24.34 PS.reconfigure :fast @14:11:24.46 PS.seek 2.5ml @14:11:41.06 Intake @15.5psia, vacuum from 10.0 to 13.0psi @14:11:41.08 SS.reconfigure :inhaling 0.43ml/s @14:11:41.12 SS.seek 25ml @14:11:44.54 Stroke completed -- waiting for pressure to equalize ... @14:11:44.55 SS.reconfigure :exhale @14:11:44.59 SS.hold @14:11:48.15 PSV.dial PTV @14:11:48.89 delay 5 seconds @14:11:53.90 PSV.dial PSR @14:11:54.66 delay 5 seconds @14:11:58.85 Intake.select :close @14:11:58.99 SSV.dial :exhaust @14:11:59.67 PSV.dial PTV @14:11:59.74 Exhaust.select :open @14:11:59.85 SS.seek 0ml @14:12:00.42 delay 5 seconds @14:12:05.44 PSV.dial :air @14:12:06.21 PS.seek 0ml @14:12:08.85 Exhaust.select :close @14:12:08.96 SSV.dial CBV @14:12:08.98 CBV.dial SSV @14:12:09.74 Allowing 130s for 25.0ml of remaining 700.0ml @14:12:09.76 Intake.select :open @14:12:11.91 Intake @15.7psia, vacuum from 10.0 to 13.0psi @14:12:11.93 SS.reconfigure :inhaling 0.43ml/s @14:12:11.97 SS.seek 25ml @14:12:29.65 getPMLReagent08 @14:12:29.67 PRV.dial :air @14:12:30.11 delay 5 seconds @14:12:35.13 PTV.dial :PRVmixing @14:12:36.44 delay 5 seconds @14:12:41.46 PRV.dial :ab2 @14:12:41.90 PTV.dial :PRVmixing @14:12:41.92 PSV.dial :mixloop @14:12:42.38 PS.reconfigure :default @14:12:42.42 PS.seek 0.3ml @14:12:42.52 Vacuum too high -- waiting up to 40 seconds @14:12:42.53 SS.reconfigure :exhale @14:12:42.57 SS.hold @14:12:46.37 delay 2 seconds @14:12:48.39 PRV.dial :air @14:12:48.90 PS.seek 0.55ml @14:12:52.22 addPMLReagent08 @14:12:52.23 PSV.dial :air @14:12:52.71 PS.seek 2ml @14:12:53.82 Intake @15.3psia, vacuum from 9.8 to 12.8psi @14:12:53.84 SS.reconfigure :inhaling 0.38ml/s @14:12:53.88 SS.seek 25ml @14:13:08.37 PSV.dial :PML @14:13:08.84 PTV.dial :mixing @14:13:09.59 PBV.dial :waste1 @14:13:09.99 PS.seek 1.85ml @14:13:12.34 PBV.dial between PSV and waste1 @14:13:12.71 PS.seek 0ml @14:13:28.79 Stroke completed -- waiting for pressure to equalize ... @14:13:28.80 SS.reconfigure :exhale @14:13:28.84 SS.hold @14:13:32.43 delay 2 seconds @14:13:34.87 delay 8:00 @14:14:03.52 Intake.select :close @14:14:03.66 SSV.dial :exhaust @14:14:04.40 Exhaust.select :open @14:14:04.51 SS.seek 0ml @14:14:13.38 Exhaust.select :close @14:14:13.49 SSV.dial CBV @14:14:13.52 CBV.dial SSV @14:14:14.27 Allowing 130s for 25.0ml of remaining 675.0ml @14:14:14.29 Intake.select :open @14:14:16.44 Intake @15.7psia, vacuum from 10.0 to 13.0psi @14:14:16.46 SS.reconfigure :inhaling 0.38ml/s @14:14:16.50 SS.seek 25ml @14:14:48.86 Vacuum too high -- waiting up to 40 seconds @14:14:48.87 SS.reconfigure :exhale @14:14:48.92 SS.hold @14:15:03.17 Intake @15.4psia, vacuum from 9.9 to 12.9psi @14:15:03.19 SS.reconfigure :inhaling 0.34ml/s @14:15:03.23 SS.seek 25ml @14:15:43.08 Vacuum too high -- waiting up to 40 seconds @14:15:43.09 SS.reconfigure :exhale @14:15:43.13 SS.hold @14:16:06.17 Intake @15.6psia, vacuum from 10.0 to 13.0psi @14:16:06.19 SS.reconfigure :inhaling 0.31ml/s @14:16:06.23 SS.seek 25ml @14:16:10.28 Stroke completed -- waiting for pressure to equalize ... @14:16:10.30 SS.reconfigure :exhale @14:16:10.34 SS.hold @14:16:24.30 SS.reconfigure :default @14:16:24.34 SS.hold @14:16:24.46 Intake.select :close @14:16:25.19 ShallowSampler::Clogged in WCR -- Filtering too Slowly after 350.0ml @14:16:25.25 SSV.dial :exhaust @14:16:26.00 Exhaust.select :open @14:16:26.11 SS.reconfigure :exhale @14:16:26.16 SS.seek 0ml @14:16:35.17 Exhaust.select :close @14:16:35.28 Sampled 350.0ml @14:16:35.30 Drying puck before fixing for 20:00 @14:16:35.31 CBV.dial :waste2b @14:16:36.06 delay 1:00 @14:17:36.08 CSV.dial :air @14:17:36.53 CS.reconfigure :fast @14:17:36.57 CS.seek 10ml @14:18:00.84 Pumping wcr puck from tube 4 up to 35psi... @14:18:00.85 CBV.dial SSV @14:18:02.15 CBV.dial between CSV and waste2b @14:18:03.08 CSV.dial between air and CTV @14:18:03.41 CS.seek 7.267ml @14:18:10.47 CTV.dial CSV,avoiding: :air @14:18:10.49 CSV.dial CTV @14:18:11.29 CS.reconfigure :pressurize @14:18:11.34 CS.seek 0ml @14:18:30.05 CBV.dial :waste2b @14:18:30.37 CS.reconfigure :default @14:18:30.42 CS.seek 0ml @14:18:31.71 delay 1:00 @14:19:31.73 CTV.dial :air,avoiding: :intake @14:19:33.06 Fix #1 of 2 (with RNAlater) for 10:00 @14:19:33.07 CSV.dial CSR @14:19:33.56 Pulling 2ml of CSR.RNAlater @14:19:33.57 CSR.select :RNAlater @14:19:33.68 CS.seek 2ml @14:19:39.55 delay 5 seconds @14:19:44.57 CSR.select :air @14:19:44.69 CS.reconfigure :fast @14:19:44.73 CS.seek 10ml @14:20:04.15 Pumping wcr puck from tube 4 up to 35psi... @14:20:04.16 CBV.dial SSV @14:20:05.45 CBV.dial between CSV and waste2b @14:20:06.37 CSV.dial between air and CTV @14:20:06.99 CS.seek 7.166ml @14:20:14.21 CTV.dial CSV,avoiding: :air @14:20:14.24 CSV.dial CTV @14:20:15.60 CS.reconfigure :pressurize @14:20:15.65 CS.seek 0ml @14:20:34.45 CSV.dial :air @14:20:34.91 CS.reconfigure :fast @14:20:34.96 CS.seek 10ml @14:20:58.94 CSV.dial between air and CTV @14:20:59.26 CS.seek 4.055ml @14:21:13.37 CTV.dial CSV,avoiding: :air @14:21:13.39 CSV.dial CTV @14:21:13.70 CS.reconfigure :pressurize @14:21:13.74 CS.seek 0ml @14:21:24.61 CSV.dial :air @14:21:25.07 CS.reconfigure :fast @14:21:25.11 CS.seek 10ml @14:21:34.98 recoverPRVreagent08 @14:21:34.99 PTV.dial :mixing @14:21:35.00 PBV.dial :waste1 @14:21:35.35 delay 30 seconds @14:21:49.20 CSV.dial between air and CTV @14:21:49.51 CS.seek 4.326ml @14:22:02.99 CTV.dial CSV,avoiding: :air @14:22:03.01 CSV.dial CTV @14:22:03.32 CS.reconfigure :pressurize @14:22:03.37 CS.seek 0ml @14:22:05.36 PBV.dial between PSV and waste1 @14:22:05.72 PSV.dial :air @14:22:06.20 PS.reconfigure :fast @14:22:06.24 PS.seek 2.5ml @14:22:15.42 CSV.dial :air @14:22:15.88 CS.reconfigure :fast @14:22:15.93 CS.seek 10ml @14:22:30.11 PSV.dial :mixloop @14:22:30.58 delay 2 seconds @14:22:32.60 PSV.dial :air @14:22:33.06 delay 2 seconds @14:22:35.08 PSV.dial :mixloop @14:22:35.55 delay 2 seconds @14:22:37.57 PSV.dial :air @14:22:38.04 PS.seek 0ml @14:22:40.04 CSV.dial between air and CTV @14:22:40.38 CS.seek 4.628ml @14:22:53.21 CTV.dial CSV,avoiding: :air @14:22:53.23 CSV.dial CTV @14:22:53.54 CS.reconfigure :pressurize @14:22:53.59 CS.seek 0ml @14:23:01.33 washCycleDA #1 of 3 @14:23:01.34 PSR.select :air @14:23:01.36 PSV.dial PSR @14:23:02.71 Pulling 0.5ml of PSR.wash @14:23:02.72 PSR.select :wash @14:23:02.83 PS.reconfigure :default @14:23:02.87 PS.seek 0.5ml @14:23:05.92 CSV.dial :air @14:23:06.38 CS.reconfigure :default @14:23:06.43 CS.hold @14:23:06.49 ShallowSampler::Error in MAIN -- Puck.from 4, :wcr did not reach 35psi after pumping in 40ml of gas @14:23:06.50 Waiting to Resume from Checkpoint "Pumping wcr puck from tube 4 up to 35psi..." @14:23:06.51 BadNews.email "Puck.from 4, :wcr did not reach 35psi after pumping in 40ml of gas",Subject: "ShallowSampler::Error in MAIN" @14:23:08.83 delay 5 seconds @14:23:13.20 upsync @14:23:13.85 PSR.select :air @14:23:13.96 PS.seek 2ml @14:23:30.30 PTV.dial :puck @14:23:31.04 PSV.dial PTV @14:23:31.89 PBV.dial :waste1 @14:23:32.33 PS.seek 1.5ml @14:23:38.23 delay 3 seconds @14:23:41.25 PBV.dial between PSV and waste1 @14:23:41.63 PS.seek 0ml @14:23:57.83 delay 2:00 @14:25:57.85 recoverPSRreagent08 @14:25:57.86 PTV.dial :puck @14:25:57.88 PBV.dial :waste1 @14:25:58.25 delay 30 seconds @14:26:28.27 PBV.dial between PSV and waste1 @14:26:28.64 PSR.select :air @14:26:28.66 PSV.dial PSR @14:26:29.41 PS.reconfigure :fast @14:26:29.45 PS.seek 2.5ml @14:26:53.01 PSV.dial PTV @14:26:53.76 delay 5 seconds @14:26:58.78 PSV.dial PSR @14:26:59.53 delay 5 seconds @14:27:04.55 PSV.dial PTV @14:27:05.42 delay 5 seconds @14:27:10.44 PSV.dial :air @14:27:11.21 PS.seek 0ml @14:27:34.26 washCycleDA #2 of 3 @14:27:34.27 PSR.select :air @14:27:34.29 PSV.dial PSR @14:27:35.64 Pulling 0.5ml of PSR.wash @14:27:35.65 PSR.select :wash @14:27:35.76 PS.reconfigure :default @14:27:35.81 PS.seek 0.5ml @14:27:41.67 delay 5 seconds @14:27:46.69 PSR.select :air @14:27:46.80 PS.seek 2ml @14:28:03.10 PTV.dial :puck @14:28:03.12 PSV.dial PTV @14:28:03.84 PBV.dial :waste1 @14:28:04.27 PS.seek 1.5ml @14:28:10.20 delay 3 seconds @14:28:13.21 PBV.dial between PSV and waste1 @14:28:13.60 PS.seek 0ml @14:28:30.23 delay 2:00 @14:30:30.26 recoverPSRreagent08 @14:30:30.27 PTV.dial :puck @14:30:30.28 PBV.dial :waste1 @14:30:30.65 delay 30 seconds @14:31:00.68 PBV.dial between PSV and waste1 @14:31:01.04 PSR.select :air @14:31:01.06 PSV.dial PSR @14:31:01.81 PS.reconfigure :fast @14:31:01.85 PS.seek 2.5ml @14:31:25.29 PSV.dial PTV @14:31:26.04 delay 5 seconds @14:31:31.06 PSV.dial PSR @14:31:31.80 delay 5 seconds @14:31:36.82 PSV.dial PTV @14:31:37.69 delay 5 seconds @14:31:42.71 PSV.dial :air @14:31:43.48 PS.seek 0ml @14:32:06.57 washCycleDA #3 of 3 @14:32:06.58 PSR.select :air @14:32:06.60 PSV.dial PSR @14:32:07.95 Pulling 0.5ml of PSR.wash @14:32:07.96 PSR.select :wash @14:32:08.07 PS.reconfigure :default @14:32:08.11 PS.seek 0.5ml @14:32:14.07 delay 5 seconds @14:32:19.09 PSR.select :air @14:32:19.20 PS.seek 2ml @14:32:35.41 PTV.dial :puck @14:32:35.42 PSV.dial PTV @14:32:36.14 PBV.dial :waste1 @14:32:36.55 PS.seek 1.5ml @14:32:42.41 delay 3 seconds @14:32:45.43 PBV.dial between PSV and waste1 @14:32:45.80 PS.seek 0ml @14:33:02.13 delay 2:00 @14:35:02.16 recoverPSRreagent08 @14:35:02.17 PTV.dial :puck @14:35:02.18 PBV.dial :waste1 @14:35:02.53 delay 30 seconds @14:35:32.56 PBV.dial between PSV and waste1 @14:35:32.91 PSR.select :air @14:35:32.93 PSV.dial PSR @14:35:33.68 PS.reconfigure :fast @14:35:33.72 PS.seek 2.5ml @14:35:57.19 PSV.dial PTV @14:35:57.94 delay 5 seconds @14:36:02.96 PSV.dial PSR @14:36:03.70 delay 5 seconds @14:36:08.72 PSV.dial PTV @14:36:09.59 delay 5 seconds @14:36:14.61 PSV.dial :air @14:36:15.38 PS.seek 0ml @14:36:38.37 Gate.power :camera,:ON @14:36:38.39 Mixing DA Substrates @14:36:38.40 PSR.select :air @14:36:38.41 PSV.dial PSR @14:36:39.91 Pulling 0.25ml of PSR.sub1 @14:36:39.94 PSR.select :sub1 @14:36:40.11 PS.reconfigure :default @14:36:40.24 PS.seek 0.25ml @14:36:43.66 delay 5 seconds @14:36:48.73 Pulling 0.25ml of PSR.sub2 @14:36:48.74 PSR.select :sub2 @14:36:48.85 PS.seek 0.5ml @14:36:52.28 delay 5 seconds @14:36:57.30 PSR.select :air @14:36:57.41 PS.seek 2ml @14:37:13.75 PTV.dial :puck @14:37:13.76 PSV.dial PTV @14:37:14.50 PBV.dial :waste1 @14:37:14.92 PS.seek 1.5ml @14:37:20.87 delay 3 seconds @14:37:23.89 PBV.dial between PSV and waste1 @14:37:24.27 PS.seek 0ml @14:37:40.47 delay 10 seconds @14:37:50.49 recoverPSRreagent08 @14:37:50.50 PTV.dial :puck @14:37:50.51 PBV.dial :waste1 @14:37:50.87 delay 30 seconds @14:38:20.90 PBV.dial between PSV and waste1 @14:38:21.26 PSR.select :air @14:38:21.28 PSV.dial PSR @14:38:22.05 PS.reconfigure :fast @14:38:22.09 PS.seek 2.5ml @14:38:46.03 PSV.dial PTV @14:38:46.79 delay 5 seconds @14:38:51.81 PSV.dial PSR @14:38:52.56 delay 5 seconds @14:38:57.58 PSV.dial PTV @14:38:58.34 delay 5 seconds @14:39:03.36 PSV.dial :air @14:39:04.13 PS.seek 0ml @14:39:27.65 Additional air clear @14:39:27.66 PSR.select :air @14:39:27.68 PSV.dial PSR @14:39:29.05 Pulling 2ml of PSR.air @14:39:29.06 PSR.select :air @14:39:29.08 PS.reconfigure :default @14:39:29.12 PS.seek 2ml @14:39:50.34 delay 5 seconds @14:39:55.36 PTV.dial :puck @14:39:55.38 PSV.dial PTV @14:39:56.12 PBV.dial :waste1 @14:39:56.54 PS.seek 1.5ml @14:40:02.46 delay 3 seconds @14:40:05.48 PBV.dial between PSV and waste1 @14:40:05.86 delay 1 second @14:40:06.87 PSV.dial :waste1 @14:40:07.95 PS.seek 0ml @14:40:24.20 Processing Puck Heater at 27.9C before imaging @14:40:24.21 PH.coast @14:40:24.33 Imaging da puck in Processing Clamp while storing it to tube 3 @14:40:24.98 SC.reconfigure :tight @14:40:25.03 SC.seek 3 @14:40:28.62 SC.configure :limp @14:40:28.82 Elbow.reconfigure :tight @14:40:28.86 Elbow.seek 3 @14:40:30.63 Forearm.reconfigure :tight @14:40:30.68 Forearm.seek 3 + 450 counts @14:40:31.67 SE.reconfigure :pick @14:40:31.72 SE.seek :beamBreak @14:40:33.37 SE.configure :default @14:40:33.52 Elbow.reconfigure :limp @14:40:33.56 Forearm.reconfigure :loose @14:40:33.60 Forearm.seek 3 @14:40:33.85 Elbow.hold @14:40:33.91 Elbow.reconfigure :tight @14:40:34.00 SE.seek 23.11 Pucks below Top Plate @14:40:34.43 Forearm.force -20 @14:40:34.46 Forearm.seek 3 + 317 counts @14:40:34.74 Forearm.reconfigure :tight @14:40:34.79 Forearm.seek 3 + 450 counts @14:40:34.99 SE.seek 24.11 Pucks below Top Plate @14:40:35.61 Forearm.reconfigure :loose @14:40:35.65 Forearm.seek :retract @14:40:36.57 Elbow.seek PC @14:40:39.04 Elbow.reconfigure :loose @14:40:39.13 Forearm.seek PC @14:40:40.09 Hand.close @14:40:40.67 PC.seek :open @14:40:45.46 Forearm.seek :retract @14:40:46.40 Elbow.reconfigure :tight @14:40:46.44 Elbow.seek 3 @14:40:49.05 Forearm.reconfigure :tight @14:40:49.10 Forearm.seek 3 + 450 counts @14:40:50.09 Hand.open @14:40:50.72 SE.seek 23.76 Pucks below Top Plate @14:40:50.98 SE.reconfigure :seal @14:40:51.03 SE.seek 22.8 Pucks below Top Plate @14:40:51.56 Lights, camera, ACTION! @14:40:51.60 PuckCamera: snap -bin=2,2 -auto /var/log/esp/da23mar1614h325ml.tif @14:41:11.08 Waiting >20 seconds for da to relinquish FlushPuck @14:41:22.50 SX HX9/16 (USB:23): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 27.615 seconds /var/log/esp/da23mar1614h325ml.tif: TIFF Upload Complete @14:41:22.53 PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da23mar1614h325ml15s.tif @14:41:38.14 SX HX9/16 (USB:23): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15 seconds /var/log/esp/midres/da23mar1614h325ml15s.tif: TIFF Upload Complete @14:41:38.17 PuckCamera: snap -bin=2,2 20.0 /var/log/esp/midres/da23mar1614h325ml20s.tif @14:41:58.82 SX HX9/16 (USB:23): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 20 seconds /var/log/esp/midres/da23mar1614h325ml20s.tif: TIFF Upload Complete @14:41:58.84 Gate.power :camera,:OFF @14:41:58.86 DAfans.email "27.615 sec auto-exposure",Subject: "da23mar1614h325ml images!" @14:41:59.10 Elbow.seek 3 @14:41:59.28 Forearm.seek 3 + 450 counts @14:41:59.35 SE.reconfigure :gently @14:41:59.40 SE.seek 23.51 Pucks below Top Plate @14:42:00.08 Elbow.reconfigure :limp @14:42:00.12 Forearm.reconfigure :loose @14:42:00.18 Forearm.seek 3 @14:42:00.41 Elbow.hold @14:42:00.48 Elbow.reconfigure :tight @14:42:00.54 SE.reconfigure :default @14:42:00.59 SE.seek 23.7 Pucks below Top Plate @14:42:01.04 Forearm.force -20 @14:42:01.08 Forearm.seek 3 + 312 counts @14:42:01.36 Forearm.reconfigure :tight @14:42:01.40 Forearm.seek 3 + 450 counts @14:42:01.68 SE.seek :down @14:42:02.90 SE.stop @14:42:03.26 Forearm.reconfigure :loose @14:42:03.31 Forearm.seek :retract @14:42:04.41 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @14:42:04.77 Elbow.seek FlushPuck::Garage @14:42:06.69 Elbow.reconfigure :loose @14:42:06.87 Forearm.seek :garage @14:42:07.85 Hand.close @14:42:08.80 Forearm.seek :retract @14:42:09.89 Elbow.reconfigure :tight @14:42:09.98 Elbow.seek PC @14:42:11.33 Elbow.reconfigure :loose @14:42:11.52 Forearm.reconfigure :tight @14:42:11.60 Forearm.seek PC @14:42:13.09 PC.seek :closed @14:42:17.82 Processing Clamp onSpring/offSpring for 16/26 samples, on/off torque 1.60 @14:42:17.97 Hand.open @14:42:18.80 Forearm.reconfigure :loose @14:42:18.88 Forearm.seek :retract @14:42:19.86 PSR.select :air @14:42:19.90 PSV.dial PSR @14:42:20.51 Pulling 0.5ml of PSR.flush @14:42:20.53 PSR.select :flush @14:42:20.70 PS.seek 0.5ml @14:42:26.68 delay 5 seconds @14:42:31.72 PSR.select :air @14:42:31.94 PS.seek 2.5ml @14:42:38.21 upsync @14:42:53.24 delay 1 second @14:42:54.26 PRV.dial :waste2,avoiding: :ab2 @14:42:56.10 PTV.dial :PRVmixing @14:42:57.41 PSV.dial :mixloop @14:42:58.45 PS.seek :empty @14:43:24.99 PSR.select :air @14:43:25.01 PSV.dial PSR @14:43:26.09 Pulling 0.5ml of PSR.flush @14:43:26.10 PSR.select :flush @14:43:26.21 PS.seek 0.5ml @14:43:32.18 delay 5 seconds @14:43:37.20 PSR.select :air @14:43:37.31 PS.seek 2.5ml @14:43:58.49 delay 1 second @14:43:59.51 PBV.dial :waste1 @14:43:59.85 PTV.dial :mixing @14:44:00.60 PSV.dial :mixloop @14:44:01.65 PS.seek :empty @14:44:28.02 PSV.dial PSR @14:44:29.07 PSR.select :flush @14:44:29.18 PS.seek 1ml @14:44:40.27 PSR.select :air @14:44:40.38 PS.seek 2.5ml @14:44:56.49 PSV.dial PTV @14:44:57.35 PTV.dial :puck @14:44:58.08 PBV.dial :waste1 @14:44:58.10 PS.seek 0ml @14:45:24.42 PSV.dial PSR @14:45:25.14 PSR.select :flush @14:45:25.26 PS.seek 0.5ml @14:45:31.17 PSR.select :air @14:45:31.29 PS.seek 2.5ml @14:45:52.55 PSV.dial PTV @14:45:53.30 PBV.dial PSV @14:45:53.86 PS.seek 0ml @14:46:20.20 PSV.dial PBV @14:46:20.68 PS.seek 2.5ml @14:46:47.08 PSV.dial :waste1 @14:46:47.82 PS.seek 0ml @14:47:14.20 PSV.dial PSR @14:47:14.67 PSR.select :air @14:47:14.70 PS.seek 2.5ml @14:47:41.30 PSV.dial PTV @14:47:42.03 PBV.dial PSV @14:47:42.05 PS.seek 0ml @14:48:08.45 PSV.dial PBV @14:48:09.44 PS.seek 2.5ml @14:48:35.83 PSV.dial :waste1 @14:48:36.57 PS.seek 0ml @14:49:02.92 PSV.dial PSR @14:49:03.39 PSR.select :flush @14:49:03.50 PS.seek 1ml @14:49:14.51 PSR.select :air @14:49:14.62 PS.seek 2.5ml @14:49:30.83 PSV.dial PTV @14:49:31.56 PTV.dial :puck @14:49:31.57 PBV.dial :waste1 @14:49:32.11 PS.seek 0ml @14:49:58.61 PSV.dial PSR @14:49:59.48 PSR.select :flush @14:49:59.60 PS.seek 0.5ml @14:50:05.57 PSR.select :air @14:50:05.69 PS.seek 2.5ml @14:50:26.98 PSV.dial PTV @14:50:27.72 PBV.dial PSV @14:50:28.28 PS.seek 0ml @14:50:54.82 PSV.dial PBV @14:50:55.30 PS.seek 2.5ml @14:51:21.61 PSV.dial :waste1 @14:51:22.35 PS.seek 0ml @14:51:48.67 PSV.dial PSR @14:51:49.12 PSR.select :air @14:51:49.14 PS.seek 2.5ml @14:52:15.45 PSV.dial PTV @14:52:16.18 PBV.dial PSV @14:52:16.20 PS.seek 0ml @14:52:42.48 PSV.dial PBV @14:52:42.95 PS.seek 2.5ml @14:53:09.29 PSV.dial :waste1 @14:53:10.03 PS.seek 0ml @14:53:36.42 Garaging FlushPuck in Processing Clamp @14:53:36.49 Elbow.reconfigure :tight @14:53:36.53 Elbow.seek PC @14:53:36.64 Elbow.reconfigure :loose @14:53:36.73 Forearm.seek PC @14:53:37.71 Hand.close @14:53:38.28 PC.seek :open @14:53:42.99 Forearm.seek :retract @14:53:43.99 Elbow.reconfigure :tight @14:53:44.04 Elbow.seek :garage @14:53:45.39 Elbow.reconfigure :loose @14:53:45.48 Forearm.reconfigure :tight @14:53:45.52 Forearm.seek :garage @14:53:46.50 Hand.open @14:53:47.21 Forearm.reconfigure :loose @14:53:47.25 Forearm.seek :retract @15:11:49.53 Session Begun @15:11:53.79 -> showlog @15:12:02.42 -> showlog -150 @15:12:43.07 -> Flush @15:12:52.69 -> Hand @15:12:55.08 -> Arm @15:13:01.90 -> CC @15:13:04.83 -> PC @15:13:15.60 -> MainThread.resume @15:13:15.62
Resuming Thread (suspended since 14:20:04) @15:13:15.65 Pumping wcr puck from tube 4 up to 35psi... @15:13:15.75 CBV.dial SSV @15:13:17.20 CBV.dial between CSV and waste2b @15:13:18.17 CSV.dial between air and CTV @15:13:18.50 CS.reconfigure :fast @15:13:18.57 CS.seek 5.601ml @15:13:33.10 CTV.dial CSV,avoiding: :air @15:13:33.12
CSV.dial CTV @15:13:33.46 CS.reconfigure :pressurize @15:13:33.52 CS.seek 0ml @15:13:48.49 CSV.dial :air @15:13:48.97 CS.reconfigure :fast @15:13:49.04 CS.seek 10ml @15:14:13.21 CSV.dial between air and CTV @15:14:13.55 CS.seek 5.611ml @15:14:24.25 CTV.dial CSV,avoiding: :air @15:14:24.28
CSV.dial CTV @15:14:25.01 CS.reconfigure :pressurize @15:14:25.14 CS.seek 0ml @15:14:40.09 CSV.dial :air @15:14:40.55 CS.reconfigure :fast @15:14:40.61 CS.seek 10ml @15:15:04.83 CSV.dial between air and CTV @15:15:05.18 CS.seek 4.673ml @15:15:17.97 CTV.dial CSV,avoiding: :air @15:15:17.99
CSV.dial CTV @15:15:18.33 CS.reconfigure :pressurize @15:15:18.39 CS.seek 0ml @15:15:30.93 CSV.dial :air @15:15:31.41 CS.reconfigure :fast @15:15:31.47 CS.seek 10ml @15:15:55.64 CSV.dial between air and CTV @15:15:55.99 CS.seek 5.85ml @15:16:06.09 CTV.dial CSV,avoiding: :air @15:16:06.13
CSV.dial CTV @15:16:06.45 CS.reconfigure :pressurize @15:16:06.52 CS.seek 0ml @15:16:21.99 CSV.dial :air @15:16:22.45 CS.reconfigure :default @15:16:22.51 CS.hold @15:16:22.59 ShallowSampler::Error in MAIN -- Puck.from 4, :wcr did not reach 35psi after pumping in 40ml of gas @15:16:22.61 Waiting to Resume from Checkpoint "Pumping wcr puck from tube 4 up to 35psi..." @15:16:22.63 BadNews.email "Puck.from 4, :wcr did not reach 35psi after pumping in 40ml of gas",Subject: "ShallowSampler::Error in MAIN" @15:16:29.46 upsync @15:16:50.67 -> MainThread.resume @15:16:50.69
Resuming Thread (suspended since 14:20:04) @15:16:50.71 Pumping wcr puck from tube 4 up to 35psi... @15:16:50.82 CBV.dial SSV @15:16:52.28 CBV.dial between CSV and waste2b @15:16:53.26 CSV.dial between air and CTV @15:16:53.60 CS.reconfigure :fast @15:16:53.66 CS.seek 4.604ml @15:17:05.81 CTV.dial CSV,avoiding: :air @15:17:05.83
CSV.dial CTV @15:17:06.17 CS.reconfigure :pressurize @15:17:06.24 CS.seek 0ml @15:17:18.49 CSV.dial :air @15:17:18.97 CS.reconfigure :fast @15:17:19.04 CS.seek 10ml @15:17:43.21 CSV.dial between air and CTV @15:17:43.55 CS.seek 4.659ml @15:17:56.41 CTV.dial CSV,avoiding: :air @15:17:56.44
CSV.dial CTV @15:17:56.76 CS.reconfigure :pressurize @15:17:56.83 CS.seek 0ml @15:18:06.42 delay 10:00 @15:28:06.44 CBV.dial :waste2b @15:28:06.78 CS.reconfigure :default @15:28:06.85 CS.seek 0ml @15:28:10.36 delay 1:00 @15:29:10.38 CTV.dial :air,avoiding: :intake @15:29:11.72 Fix #2 of 2 (with RNAlater) for 10:00 @15:29:11.73 CSV.dial CSR @15:29:12.25 Pulling 2ml of CSR.RNAlater @15:29:12.27 CSR.select :RNAlater @15:29:12.84 CS.seek 2ml @15:29:18.86 delay 5 seconds @15:29:23.87 CSR.select :air @15:29:24.01 CS.reconfigure :fast @15:29:24.07 CS.seek 10ml @15:29:43.48 Pumping wcr puck from tube 4 up to 35psi... @15:29:43.50 CBV.dial SSV @15:29:44.81 CBV.dial between CSV and waste2b @15:29:45.78 CSV.dial between air and CTV @15:29:46.40 CS.seek 7.061ml @15:29:53.89 CTV.dial CSV,avoiding: :air @15:29:53.91
CSV.dial CTV @15:29:55.30 CS.reconfigure :pressurize @15:29:55.37 CS.seek 0ml @15:30:10.03 delay 10:00 @15:40:10.05 CBV.dial :waste2b @15:40:10.40 CS.reconfigure :default @15:40:10.46 CS.seek 0ml @15:40:15.13 delay 1:00 @15:41:15.16 CTV.dial :air,avoiding: :intake @15:41:16.54 Storing wcr puck in Collection Clamp to tube 3 @15:41:16.93 Elbow.reconfigure :tight @15:41:16.99 Elbow.seek 3 @15:41:18.86 Forearm.reconfigure :tight @15:41:18.93 Forearm.seek 3 + 450 counts @15:41:19.95 SE.reconfigure :pick @15:41:20.02 SE.seek :beamBreak @15:41:21.62 SE.configure :default @15:41:21.78 Elbow.reconfigure :limp @15:41:21.85 Forearm.reconfigure :loose @15:41:21.91 Forearm.seek 3 @15:41:22.08 Elbow.hold @15:41:22.16 Elbow.reconfigure :tight @15:41:22.27 SE.seek 24.1 Pucks below Top Plate @15:41:22.74 Forearm.force -20 @15:41:22.79 Forearm.seek 3 + 311 counts @15:41:23.08 Forearm.reconfigure :tight @15:41:23.15 Forearm.seek 3 + 450 counts @15:41:23.38 SE.seek 25.1 Pucks below Top Plate @15:41:24.02 Forearm.reconfigure :loose @15:41:24.08 Forearm.seek :retract @15:41:25.00 Elbow.seek CC @15:41:26.61 Elbow.reconfigure :loose @15:41:26.72 Forearm.seek CC @15:41:27.72 Hand.close @15:41:28.36 CC.seek :open @15:41:33.09 Forearm.seek :retract @15:41:34.05 Elbow.reconfigure :tight @15:41:34.11 Elbow.seek 3 @15:41:35.92 Elbow.reconfigure :limp @15:41:35.99 Forearm.seek 3 @15:41:37.00 Elbow.hold @15:41:37.09 Elbow.reconfigure :tight @15:41:37.15 Hand.open @15:41:37.84 Forearm.force -20 @15:41:37.90 Forearm.seek 3 + 314 counts @15:41:38.21 Forearm.reconfigure :tight @15:41:38.27 Forearm.seek 3 + 450 counts @15:41:38.54 SE.seek :down @15:41:39.54 SE.stop @15:41:40.15
Forearm.reconfigure :loose @15:41:40.23 Forearm.seek :retract @15:41:41.18 Starting finalPurge @15:41:41.19 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @15:41:41.40 Elbow.seek FlushPuck::Garage @15:41:43.29 Elbow.reconfigure :loose @15:41:43.40 Forearm.seek :garage @15:41:44.36 Hand.close @15:41:45.09 Forearm.seek :retract @15:41:46.10 Elbow.reconfigure :tight @15:41:46.16 Elbow.seek CC @15:41:49.21 Elbow.reconfigure :loose @15:41:49.32 Forearm.reconfigure :tight @15:41:49.38 Forearm.seek CC @15:41:50.61 CC.seek :closed @15:41:55.12 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.22 @15:41:55.21 Hand.open @15:41:55.92 Forearm.reconfigure :loose @15:41:55.98 Forearm.seek :retract @15:41:56.95 Flushing the Collection Valve::Manifold @15:41:57.09 CSV.dial CSR @15:41:57.60 Pulling 5ml of CSR.flush @15:41:57.61 CSR.select :flush @15:41:57.75 CS.seek 5ml @15:42:11.56 delay 5 seconds @15:42:16.58 CSR.select :air @15:42:16.73 CS.reconfigure :fast @15:42:16.79 CS.seek 7.5ml @15:42:23.68 CSV.dial :waste @15:42:25.08 CS.reconfigure :default @15:42:25.14 CS.seek 0ml @15:42:45.12 Flushing lower manifold's waste1 port @15:42:45.25 CSV.dial :flush @15:42:45.72 CS.reconfigure :fast @15:42:45.79 CS.seek 5ml @15:42:58.44 delay 5 seconds @15:43:03.46 CSV.dial :air @15:43:03.98 CS.seek 7.001ml @15:43:09.44 CSV.dial CTV @15:43:09.46
CTV.dial CSV @15:43:10.85 CBV.dial :waste1 @15:43:11.61 CS.seek 0ml @15:43:28.15 Flushing lower manifold's waste2 port @15:43:28.28 CSV.dial :flush @15:43:29.02 CS.seek 5ml @15:43:41.81 delay 5 seconds @15:43:46.83 CSV.dial :air @15:43:47.35 CS.seek 7ml @15:43:52.84 CSV.dial CTV @15:43:52.86
CTV.dial CSV @15:43:53.33 CBV.dial :waste2 @15:43:54.38 CS.seek 0ml @15:44:10.78 Flushing lower manifold's waste2b port @15:44:10.91 CSV.dial :flush @15:44:11.77 CS.seek 5ml @15:44:24.25 delay 5 seconds @15:44:29.26 CSV.dial :air @15:44:29.77 CS.seek 7ml @15:44:35.43 CSV.dial CTV @15:44:35.46
CTV.dial CSV @15:44:35.92 CBV.dial :waste2b @15:44:36.97 CS.seek 0ml @15:44:53.40 Disinfecting external Intake with kill @15:44:53.54 CSV.dial CSR @15:44:54.03 Pulling 7.999ml of CSR.kill @15:44:54.04 CSR.select :kill @15:44:54.18 CS.seek 8ml @15:45:13.75 delay 5 seconds @15:45:18.76 CSR.select :air @15:45:18.88 CS.seek 10ml @15:45:24.56 CSV.dial CBV @15:45:24.58
CBV.dial CSV @15:45:25.63 CTV.dial :intake @15:45:26.41 Intake.select :open @15:45:26.53 CS.seek 2ml @15:45:45.15 Intake.select :close @15:45:45.27 CTV.dial :air @15:45:46.06 CS.reconfigure :default @15:45:46.61 CS.seek 0ml @15:45:52.77 CSV.dial CSR @15:45:53.95 Pulling 5ml of CSR.flush @15:45:53.96 CSR.select :flush @15:45:54.11 CS.seek 5ml @15:46:07.72 delay 5 seconds @15:46:12.73 CSR.select :air @15:46:12.85 CS.seek 10ml @15:46:26.49 CSV.dial CBV @15:46:27.48 CS.seek 0ml @15:46:54.06 Garaging FlushPuck in Collection Clamp @15:46:54.15 Elbow.reconfigure :tight @15:46:54.21 Elbow.seek CC @15:46:54.33 Elbow.reconfigure :loose @15:46:54.44 Forearm.seek CC @15:46:55.42 Hand.close @15:46:55.98 CC.seek :open @15:47:00.60 Forearm.seek :retract @15:47:01.56 Elbow.reconfigure :tight @15:47:01.62 Elbow.seek :garage @15:47:04.53 Elbow.reconfigure :loose @15:47:04.64 Forearm.reconfigure :tight @15:47:04.70 Forearm.seek :garage @15:47:05.72 Hand.open @15:47:06.41 Forearm.reconfigure :loose @15:47:06.47 Forearm.seek :retract @15:47:07.48 Completed [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] begun 12:55:43 @15:47:07.55 Asleep after completing DA_WCR_031623 assays @15:47:07.57 GoodNews.email "{1 => 33,\n 2 => 33,\n 3 => 26,\n 4 => 7,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@15:44:54, 17% flow, 21.9C, 39% humidity, 14.9psia, 13.730V, 0.472A, 0.460A avg, 6.47W\nSampled 350.0ml",Subject: "Asleep after completing DA_WCR_031623 assays" @15:47:12.74 upsync @15:47:12.82 Session Ended @14:13:01.05UTC22-Mar-23
ESP2 v3.41 I2C Gateway v3.20 for core bus @14:13:03.66 Configuration for short puck jake 1/11/21 by brent@mbari.org /home/esp/esp2/type/shallow/jake/configure.rb @14:13:07.61 Polling Can every 10:00 @14:13:07.88 Cleanly exited at 14:10:15UTC22-Mar-23 @14:13:07.92 Loading /home/esp/esp2/mission/DA_WCR_032223.rb mission @14:13:07.97 Preparing to sleep until + 00:00:05 @14:13:07.99 GoodNews.email "Starting DA_WCR_032223 w/pucks in tube 4\n{1 => 33,\n 2 => 33,\n 3 => 26,\n 4 => 7,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@14:13:07, 16% flow, 21.9C, 41% humidity, 14.9psia, 13.773V, 0.272A, 0.254A avg, 3.75W",Subject: "DA_WCR_032223 begun" @14:13:08.03
Asleep til + 00:00:05 @14:13:08.07 Continuing at 14:13 @14:13:08.31 Gate.power :core,:OFF @14:13:08.33 Gate.power :raw,:OFF Shutdown @14:13:11.49 GoodNews.email "Then, in tube 4: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2/mission/DA_WCR_032223.rb:14\n{1 => 33,\n 2 => 33,\n 3 => 26,\n 4 => 7,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@14:13:08, 16% flow, 21.9C, 41% humidity, 14.9psia, 13.773V, 0.272A, 0.254A avg, 3.75W",Subject: "Asleep until 14:13" @14:13:12.95
Powering core bus @14:13:13.25 upsync @14:13:16.46 upsync @14:13:18.57
Gate.power :raw,:ON @14:13:19.17 CS.configure :default @14:13:19.26 PS.configure :default @14:13:19.35 SS.configure :default @14:13:19.44 CC.configure :default @14:13:19.53 PC.configure :default @14:13:19.64 Forearm.configure :loose @14:13:19.72 Elbow.configure :loose @14:13:19.83 SC.configure :loose @14:13:19.91 SE.configure :default @14:13:19.96 CH.coast @14:13:20.01 CH.configure :default @14:13:20.04 PH.coast @14:13:20.09 PH.configure :default @14:13:20.12 All dwarf controllers running firmware version 3.85 @14:13:20.23 Valve.reset @14:13:20.49 PSV.dial :air @14:13:20.53 Intake.select :close @14:13:20.56 Exhaust.select :close @14:13:20.59 CTV.dial :air @14:13:20.66 CSV.dial :waste1 @14:13:20.76
Forearm.reconfigure :loose @14:13:20.80 Forearm.home.seek :retract @14:13:21.15 SSV.dial :spit,avoiding: :exhaust @14:13:21.53 Hand.close @14:13:21.57
Elbow.jog 1500 @14:13:21.67 PS.reconfigure :default @14:13:21.71 PS.home.seek 0ml @14:13:21.97 CS.reconfigure :fast @14:13:22.00 SS.reconfigure :default @14:13:22.09 CS.home.seek 0ml @14:13:22.13 SS.home.seek 0ml @14:13:22.23
Elbow.reconfigure :loose @14:13:22.27 Elbow.home.seek :clear @14:13:25.48 SE.reconfigure :default @14:13:25.52 SE.home.seek :down @14:13:26.14 SC.reconfigure :tight @14:13:26.18 SC.home.seek :initial @14:13:30.04 PS.stop @14:13:54.52
SC.configure :limp @14:13:54.57 Forearm.seek :retract @14:13:54.62 Elbow.seek :hammer @14:13:55.65 Forearm.reconfigure :tight @14:13:55.70 Forearm.seek retracted @14:13:55.91 PC.reconfigure :default @14:13:55.93
CC.reconfigure :default @14:13:56.02 CC.home.seek :open @14:13:56.03 PC.home.seek :open @14:13:59.59
Phase [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] @14:13:59.60 Notice.email "At 14:13:59 began in tube 4: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2/mission/DA_WCR_032223.rb:14\n{1 => 33,\n 2 => 33,\n 3 => 26,\n 4 => 7,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@14:13:59, 16% flow, 22.0C, 41% humidity, 14.9psia, 13.764V, 0.309A, 0.290A avg, 4.25W",Subject: "Started DA_WCR_032223 phase initialPurge; da [1000, 1000] #da and wsr are run simultanelously" @14:13:59.62
Starting initialPurge @14:13:59.65 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:13:59.71 Hand.open @14:14:00.39 Elbow.reconfigure :tight @14:14:00.43 Elbow.seek FlushPuck::Garage @14:14:02.33 Elbow.reconfigure :loose @14:14:02.42 Forearm.reconfigure :loose @14:14:02.46 Forearm.seek :garage @14:14:03.41 Hand.close @14:14:04.14 Forearm.seek :retract @14:14:04.46 upsync @14:14:05.12
Elbow.reconfigure :tight @14:14:05.16 Elbow.seek CC @14:14:08.13 Elbow.reconfigure :loose @14:14:08.22 Forearm.reconfigure :tight @14:14:08.27 Forearm.seek CC @14:14:09.53 CC.seek :closed @14:14:14.05 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.26 @14:14:14.11 Hand.open @14:14:14.82 Forearm.reconfigure :loose @14:14:14.86 Forearm.seek :retract @14:14:15.91 Flushing the Collection Valve::Manifold @14:14:16.03 CSV.dial CSR @14:14:17.34 Pulling 4.999ml of CSR.flush @14:14:17.35 CSR.select :flush @14:14:17.46 CS.reconfigure :default @14:14:17.50 CS.seek 5ml @14:14:31.09 delay 5 seconds @14:14:36.11 CSR.select :air @14:14:36.26 CS.reconfigure :fast @14:14:36.30 CS.seek 7.5ml @14:14:43.12 CSV.dial :waste @14:14:44.37 CS.reconfigure :default @14:14:44.41 CS.seek 0ml @14:15:04.37 Purging external Intake @14:15:04.48 CSV.dial :flush @14:15:04.92 CS.reconfigure :fast @14:15:04.96 CS.seek 10ml @14:15:29.40 delay 5 seconds @14:15:34.42 CSV.dial CBV @14:15:34.44
CBV.dial CSV @14:15:35.19 CTV.dial :intake @14:15:35.97 Intake.select :open @14:15:36.08 CS.seek 0ml @14:15:59.52 Intake.select :close @14:15:59.62 CTV.dial :air @14:16:00.41 Flushing upper manifold @14:16:00.53 CSV.dial :flush @14:16:01.26 CS.seek 5ml @14:16:14.05 delay 5 seconds @14:16:19.07 CSV.dial :air @14:16:19.57 CS.seek 7ml @14:16:24.99 CSV.dial CBV @14:16:25.01
CBV.dial CSV @14:16:26.04 CTV.dial :air @14:16:26.06 CS.seek 0ml @14:16:42.58 delay 7 seconds @14:16:49.61 CS.seek 3ml @14:16:57.58 CSV.dial :waste @14:16:58.03 CS.seek 0ml @14:17:05.78 CSV.dial :flush @14:17:06.22 CS.seek 3ml @14:17:14.14 delay 5 seconds @14:17:19.16 CSV.dial :air @14:17:19.66 CS.seek 5ml @14:17:25.14 CSV.dial :air @14:17:25.16 CS.seek 0ml @14:17:37.24 delay 7 seconds @14:17:44.27 CS.seek 3ml @14:17:52.71 CSV.dial :waste @14:17:53.43 CS.seek 0ml @14:18:01.21 Garaging FlushPuck in Collection Clamp @14:18:01.28 Elbow.reconfigure :tight @14:18:01.32 Elbow.seek CC @14:18:01.70 Elbow.reconfigure :loose @14:18:01.79 Forearm.seek CC @14:18:02.71 Hand.close @14:18:03.26 CC.seek :open @14:18:07.88 Forearm.seek :retract @14:18:08.84 Elbow.reconfigure :tight @14:18:08.88 Elbow.seek :garage @14:18:11.76 Elbow.reconfigure :loose @14:18:11.86 Forearm.reconfigure :tight @14:18:11.90 Forearm.seek :garage @14:18:12.89 Hand.open @14:18:14.01 Forearm.reconfigure :loose @14:18:14.05 Forearm.seek :retract @14:18:15.00 da sampling at most 1000ml, wcr at most 1000ml @14:18:15.03 DAfans.email "Started da in tube 4 at 14:18:15UTC22-Mar-23\n{1 => 33,\n 2 => 33,\n 3 => 26,\n 4 => 7,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@14:13:59, 16% flow, 22.0C, 41% humidity, 14.9psia, 13.764V, 0.309A, 0.290A avg, 4.25W",Subject: "da sampling at most 1000ml, wcr at most 1000ml" @14:18:15.05
Loading sh1 puck from tube 4 into Collection Clamp @14:18:15.52 Elbow.reconfigure :tight @14:18:15.59 Elbow.seek 4 @14:18:17.50 Forearm.reconfigure :tight @14:18:17.54 Forearm.seek 4 + 450 counts @14:18:18.57 SE.reconfigure :pick @14:18:18.63 SE.seek :beamBreak @14:18:19.75 upsync @14:18:21.37
SE.configure :default @14:18:21.52 Elbow.seek 4 @14:18:21.70 Forearm.seek 4 + 300 counts @14:18:21.88 Forearm.reconfigure :loose @14:18:21.92 Hand.close @14:18:22.52 SE.seek :down @14:18:22.71
Forearm.seek :retract @14:18:23.68 Elbow.seek CC @14:18:24.89 SE.stop @14:18:25.25
Elbow.reconfigure :loose @14:18:25.35 Forearm.reconfigure :tight @14:18:25.39 Forearm.seek CC @14:18:26.68 CC.seek :closed @14:18:31.20 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.16 @14:18:31.21 Hand.open @14:18:31.82 Forearm.reconfigure :loose @14:18:31.86 Forearm.seek :retract @14:18:32.82 collectSample 1000ml w/bubble point of 24psi @14:18:32.84 Priming 7ml Intake.select :close @14:18:32.85 Exhaust.select :close @14:18:32.87 SS.hold @14:18:33.06 Calibrating pressure and temperature sensors @14:18:33.09 CTV.dial :air @14:18:33.10 SSV.dial :spit,avoiding: :exhaust @14:18:33.12 SS.reconfigure :fast @14:18:33.16 SS.seek 5ml @14:18:35.99 SSV.dial CBV @14:18:36.01
CBV.dial SSV @14:18:37.32 delay 3.8 seconds @14:18:41.23 SSV.dial :spit @14:18:42.52 SS.seek 0ml @14:18:45.25 SSV.dial CBV @14:18:46.55 CTV.dial :intake @14:18:47.32 SSV.dial CBV @14:18:47.34
CBV.dial SSV @14:18:47.35 Allowing 40s for 7.0ml of remaining 7.0ml @14:18:47.36 Intake.select :open @14:18:49.52 Intake @15.1psia, vacuum from 9.6 to 12.6psi @14:18:49.54 SS.reconfigure :inhaling 2.20ml/s @14:18:49.58 SS.seek 7ml @14:18:56.28 Stroke completed -- waiting for pressure to equalize ... @14:18:56.29 SS.reconfigure :exhale @14:18:56.33 SS.hold @14:18:57.19 Intake.select :close @14:18:57.33 SSV.dial :exhaust @14:18:58.08 Exhaust.select :open @14:18:58.19 SS.seek 0ml @14:19:02.23 Exhaust.select :close @14:19:02.34 Sampling 1000.0ml Intake.select :close @14:19:02.35 Exhaust.select :close @14:19:02.36 SS.reconfigure :default @14:19:02.40 SS.hold @14:19:02.58 CTV.dial :intake @14:19:02.60 SSV.dial CBV @14:19:02.63
CBV.dial SSV @14:19:03.38 Allowing 130s for 25.0ml of remaining 1000.0ml @14:19:03.39 Intake.select :open @14:19:05.54 Intake @15.2psia, vacuum from 9.7 to 12.7psi @14:19:05.55 SS.reconfigure :inhaling 2.20ml/s @14:19:05.59 SS.seek 25ml @14:19:20.42 Stroke completed -- waiting for pressure to equalize ... @14:19:20.43 SS.reconfigure :exhale @14:19:20.47 SS.hold @14:19:28.93 Intake.select :close @14:19:29.07 SSV.dial :exhaust @14:19:29.81 Exhaust.select :open @14:19:29.92 SS.seek 0ml @14:19:38.86 Exhaust.select :close @14:19:38.96 SSV.dial CBV @14:19:38.98
CBV.dial SSV @14:19:39.74 Allowing 130s for 25.0ml of remaining 975.0ml Intake.select :open @14:19:41.89 Intake @15.9psia, vacuum from 10.0 to 13.0psi @14:19:41.91 SS.reconfigure :inhaling 2.20ml/s @14:19:41.95 SS.seek 25ml @14:19:51.05 Vacuum too high -- waiting up to 40 seconds @14:19:51.06 SS.reconfigure :exhale @14:19:51.10 SS.hold @14:19:53.02 Intake @13.7psia, vacuum from 8.2 to 11.2psi @14:19:53.04 SS.reconfigure :inhaling 1.98ml/s @14:19:53.08 SS.seek 25ml @14:19:59.90 Stroke completed -- waiting for pressure to equalize ... @14:19:59.91 SS.reconfigure :exhale @14:19:59.95 SS.hold @14:20:06.34 Intake.select :close @14:20:06.48 SSV.dial :exhaust @14:20:07.22 Exhaust.select :open @14:20:07.32 SS.seek 0ml @14:20:16.09 Exhaust.select :close @14:20:16.19 SSV.dial CBV @14:20:16.22
CBV.dial SSV @14:20:16.97 Allowing 130s for 25.0ml of remaining 950.0ml @14:20:16.98 Intake.select :open @14:20:19.13 Intake @16.3psia, vacuum from 10.0 to 13.0psi @14:20:19.15 SS.reconfigure :inhaling 1.98ml/s @14:20:19.19 SS.seek 25ml @14:20:27.55 Vacuum too high -- waiting up to 40 seconds @14:20:27.56 SS.reconfigure :exhale @14:20:27.60 SS.hold @14:20:28.23 Intake @13.4psia, vacuum from 7.9 to 10.9psi @14:20:28.24 SS.reconfigure :inhaling 1.79ml/s @14:20:28.29 SS.seek 25ml @14:20:37.28 Stroke completed -- waiting for pressure to equalize ... @14:20:37.29 SS.reconfigure :exhale @14:20:37.33 SS.hold @14:20:44.68 Intake.select :close @14:20:44.81 SSV.dial :exhaust @14:20:45.56 Exhaust.select :open @14:20:45.67 SS.seek 0ml @14:20:54.36 Exhaust.select :close @14:20:54.46 SSV.dial CBV @14:20:54.48
CBV.dial SSV @14:20:55.23 Allowing 130s for 25.0ml of remaining 925.0ml @14:20:55.24 Intake.select :open @14:20:57.39 Intake @16.2psia, vacuum from 10.0 to 13.0psi @14:20:57.41 SS.reconfigure :inhaling 1.79ml/s @14:20:57.45 SS.seek 25ml @14:21:06.65 Vacuum too high -- waiting up to 40 seconds @14:21:06.66 SS.reconfigure :exhale @14:21:06.70 SS.hold @14:21:07.54 Intake @13.6psia, vacuum from 8.1 to 11.1psi @14:21:07.55 SS.reconfigure :inhaling 1.61ml/s @14:21:07.59 SS.seek 25ml @14:21:17.07 Stroke completed -- waiting for pressure to equalize ... @14:21:17.08 SS.reconfigure :exhale @14:21:17.12 SS.hold @14:21:24.48 Intake.select :close @14:21:24.61 SSV.dial :exhaust @14:21:25.36 Exhaust.select :open @14:21:25.46 SS.seek 0ml @14:21:34.14 Exhaust.select :close @14:21:34.24 SSV.dial CBV @14:21:34.26
CBV.dial SSV @14:21:35.01 Allowing 130s for 25.0ml of remaining 900.0ml @14:21:35.02 Intake.select :open @14:21:37.18 Intake @16.1psia, vacuum from 10.0 to 13.0psi @14:21:37.20 SS.reconfigure :inhaling 1.61ml/s @14:21:37.24 SS.seek 25ml @14:21:49.43 Vacuum too high -- waiting up to 40 seconds @14:21:49.44 SS.reconfigure :exhale @14:21:49.48 SS.hold @14:21:51.29 Intake @13.7psia, vacuum from 8.2 to 11.2psi @14:21:51.30 SS.reconfigure :inhaling 1.45ml/s @14:21:51.35 SS.seek 25ml @14:21:59.20 Stroke completed -- waiting for pressure to equalize ... @14:21:59.21 SS.reconfigure :exhale @14:21:59.25 SS.hold @14:22:06.05 Intake.select :close @14:22:06.19 SSV.dial :exhaust @14:22:06.93 Exhaust.select :open @14:22:07.04 SS.seek 0ml @14:22:15.96 Exhaust.select :close @14:22:16.07 SSV.dial CBV @14:22:16.12
CBV.dial SSV @14:22:16.83 Allowing 130s for 25.0ml of remaining 875.0ml @14:22:16.84 Intake.select :open @14:22:18.99 Intake @16.1psia, vacuum from 10.0 to 13.0psi @14:22:19.00 SS.reconfigure :inhaling 1.45ml/s @14:22:19.05 SS.seek 25ml @14:22:33.96 Vacuum too high -- waiting up to 40 seconds @14:22:33.97 SS.reconfigure :exhale @14:22:34.01 SS.hold @14:22:37.10 Intake @14.4psia, vacuum from 8.9 to 11.9psi @14:22:37.11 SS.reconfigure :inhaling 1.30ml/s @14:22:37.16 SS.seek 25ml @14:22:43.45 Stroke completed -- waiting for pressure to equalize ... @14:22:43.46 SS.reconfigure :exhale @14:22:43.50 SS.hold @14:22:49.12 Intake.select :close @14:22:49.26 SSV.dial :exhaust @14:22:50.00 Exhaust.select :open @14:22:50.10 SS.seek 0ml @14:22:58.85 Exhaust.select :close @14:22:58.96 SSV.dial CBV @14:22:59.01
CBV.dial SSV @14:22:59.71 Allowing 130s for 25.0ml of remaining 850.0ml @14:22:59.72 Intake.select :open @14:23:01.88 Intake @16.1psia, vacuum from 10.0 to 13.0psi @14:23:01.89 SS.reconfigure :inhaling 1.30ml/s @14:23:01.93 SS.seek 25ml @14:23:18.73 Vacuum too high -- waiting up to 40 seconds @14:23:18.74 SS.reconfigure :exhale @14:23:18.79 SS.hold @14:23:20.49 Intake @14.0psia, vacuum from 8.5 to 11.5psi @14:23:20.51 SS.reconfigure :inhaling 1.17ml/s @14:23:20.55 SS.seek 25ml @14:23:27.15 Stroke completed -- waiting for pressure to equalize ... @14:23:27.16 SS.reconfigure :exhale @14:23:27.20 SS.hold @14:23:32.73 Intake.select :close @14:23:32.87 SSV.dial :exhaust @14:23:33.60 Exhaust.select :open @14:23:33.71 SS.seek 0ml @14:23:42.65 Exhaust.select :close @14:23:42.75 SSV.dial CBV @14:23:42.77
CBV.dial SSV @14:23:43.53 Allowing 130s for 25.0ml of remaining 825.0ml @14:23:43.54 Intake.select :open @14:23:45.69 Intake @16.1psia, vacuum from 10.0 to 13.0psi @14:23:45.70 SS.reconfigure :inhaling 1.17ml/s @14:23:45.75 SS.seek 25ml @14:24:09.76 Stroke completed -- waiting for pressure to equalize ... @14:24:09.77 SS.reconfigure :exhale @14:24:09.81 SS.hold @14:24:17.27 Intake.select :close @14:24:17.40 SSV.dial :exhaust @14:24:18.15 Exhaust.select :open @14:24:18.26 SS.seek 0ml @14:24:26.98 Exhaust.select :close @14:24:27.08 SSV.dial CBV @14:24:27.10
CBV.dial SSV @14:24:27.85 Allowing 130s for 25.0ml of remaining 800.0ml @14:24:27.86 Intake.select :open @14:24:30.02 Intake @16.1psia, vacuum from 10.0 to 13.0psi @14:24:30.03 SS.reconfigure :inhaling 1.17ml/s @14:24:30.07 SS.seek 25ml @14:24:50.83 Vacuum too high -- waiting up to 40 seconds @14:24:50.84 SS.reconfigure :exhale @14:24:50.88 SS.hold @14:24:54.12 Intake @14.8psia, vacuum from 9.3 to 12.3psi @14:24:54.14 SS.reconfigure :inhaling 1.06ml/s @14:24:54.18 SS.seek 25ml @14:24:58.65 Stroke completed -- waiting for pressure to equalize ... @14:24:58.66 SS.reconfigure :exhale @14:24:58.70 SS.hold @14:25:03.45 Intake.select :close @14:25:03.58 SSV.dial :exhaust @14:25:04.32 Exhaust.select :open @14:25:04.43 SS.seek 0ml @14:25:13.19 Exhaust.select :close @14:25:13.30 SSV.dial CBV @14:25:13.33
CBV.dial SSV @14:25:14.06 Allowing 130s for 25.0ml of remaining 775.0ml @14:25:14.07 Intake.select :open @14:25:16.23 Intake @16.1psia, vacuum from 10.0 to 13.0psi @14:25:16.25 SS.reconfigure :inhaling 1.06ml/s @14:25:16.29 SS.seek 25ml @14:25:42.66 Stroke completed -- waiting for pressure to equalize ... @14:25:42.67 SS.reconfigure :exhale @14:25:42.71 SS.hold @14:25:50.81 Intake.select :close @14:25:50.94 SSV.dial :exhaust @14:25:51.66 Exhaust.select :open @14:25:51.77 SS.seek 0ml @14:26:00.80 Exhaust.select :close @14:26:00.90 SSV.dial CBV @14:26:00.93
CBV.dial SSV @14:26:01.70 Allowing 130s for 25.0ml of remaining 750.0ml @14:26:01.71 Intake.select :open @14:26:03.86 Intake @16.1psia, vacuum from 10.0 to 13.0psi @14:26:03.88 SS.reconfigure :inhaling 1.06ml/s @14:26:03.92 SS.seek 25ml @14:26:27.36 Vacuum too high -- waiting up to 40 seconds @14:26:27.37 SS.reconfigure :exhale @14:26:27.41 SS.hold @14:26:31.57 Intake @15.2psia, vacuum from 9.7 to 12.7psi @14:26:31.58 SS.reconfigure :inhaling 0.95ml/s @14:26:31.63 SS.seek 25ml @14:26:34.85 Stroke completed -- waiting for pressure to equalize ... @14:26:34.86 SS.reconfigure :exhale @14:26:34.90 SS.hold @14:26:39.77 Intake.select :close @14:26:39.91 SSV.dial :exhaust @14:26:40.65 Exhaust.select :open @14:26:40.75 SS.seek 0ml @14:26:49.89 Exhaust.select :close @14:26:50.00 SSV.dial CBV @14:26:50.02
CBV.dial SSV @14:26:50.77 Allowing 130s for 25.0ml of remaining 725.0ml @14:26:50.78 Intake.select :open @14:26:52.93 Intake @16.2psia, vacuum from 10.0 to 13.0psi @14:26:52.95 SS.reconfigure :inhaling 0.95ml/s @14:26:52.99 SS.seek 25ml @14:27:19.26 Vacuum too high -- waiting up to 40 seconds @14:27:19.27 SS.reconfigure :exhale @14:27:19.31 SS.hold @14:27:23.46 Intake @15.2psia, vacuum from 9.7 to 12.7psi @14:27:23.48 SS.reconfigure :inhaling 0.87ml/s @14:27:23.52 SS.seek 25ml @14:27:26.52 Stroke completed -- waiting for pressure to equalize ... @14:27:26.53 SS.reconfigure :exhale @14:27:26.57 SS.hold @14:27:31.43 Intake.select :close @14:27:31.57 SSV.dial :exhaust @14:27:32.30 Exhaust.select :open @14:27:32.41 SS.seek 0ml @14:27:41.52 Exhaust.select :close @14:27:42.12 SSV.dial CBV @14:27:42.14
CBV.dial SSV @14:27:42.90 Allowing 130s for 25.0ml of remaining 700.0ml @14:27:42.91 Intake.select :open @14:27:45.06 Intake @16.2psia, vacuum from 10.0 to 13.0psi @14:27:45.08 SS.reconfigure :inhaling 0.87ml/s @14:27:45.12 SS.seek 25ml @14:28:16.11 Stroke completed -- waiting for pressure to equalize ... @14:28:16.12 SS.reconfigure :exhale @14:28:16.16 SS.hold @14:28:25.91 Intake.select :close @14:28:26.04 SSV.dial :exhaust @14:28:26.79 Exhaust.select :open @14:28:26.90 SS.seek 0ml @14:28:35.69 Exhaust.select :close @14:28:35.79 SSV.dial CBV @14:28:35.81
CBV.dial SSV @14:28:36.57 Allowing 130s for 25.0ml of remaining 675.0ml @14:28:36.58 Intake.select :open @14:28:38.73 Intake @16.1psia, vacuum from 10.0 to 13.0psi @14:28:38.74 SS.reconfigure :inhaling 0.87ml/s @14:28:38.79 SS.seek 25ml @14:29:08.32 Vacuum too high -- waiting up to 40 seconds @14:29:08.33 SS.reconfigure :exhale @14:29:08.37 SS.hold @14:29:12.53 Intake @15.1psia, vacuum from 9.6 to 12.6psi @14:29:12.54 SS.reconfigure :inhaling 0.78ml/s @14:29:12.59 SS.seek 25ml @14:29:14.99 Stroke completed -- waiting for pressure to equalize ... @14:29:15.00 SS.reconfigure :exhale @14:29:15.04 SS.hold @14:29:20.65 Intake.select :close @14:29:20.78 SSV.dial :exhaust @14:29:21.52 Exhaust.select :open @14:29:21.62 SS.seek 0ml @14:29:30.38 Exhaust.select :close @14:29:30.48 SSV.dial CBV @14:29:30.50
CBV.dial SSV @14:29:31.25 Allowing 130s for 25.0ml of remaining 650.0ml @14:29:31.26 Intake.select :open @14:29:33.41 Intake @16.2psia, vacuum from 10.0 to 13.0psi @14:29:33.43 SS.reconfigure :inhaling 0.78ml/s @14:29:33.47 SS.seek 25ml @14:30:08.64 Stroke completed -- waiting for pressure to equalize ... @14:30:08.65 SS.reconfigure :exhale @14:30:08.69 SS.hold @14:30:18.35 Intake.select :close @14:30:18.49 SSV.dial :exhaust @14:30:19.24 Exhaust.select :open @14:30:19.35 SS.seek 0ml @14:30:28.09 Exhaust.select :close @14:30:28.20 SSV.dial CBV @14:30:28.25
CBV.dial SSV @14:30:28.96 Allowing 130s for 25.0ml of remaining 625.0ml @14:30:28.97 Intake.select :open @14:30:31.12 Intake @16.1psia, vacuum from 10.0 to 13.0psi @14:30:31.14 SS.reconfigure :inhaling 0.78ml/s @14:30:31.18 SS.seek 25ml @14:31:06.72 Stroke completed -- waiting for pressure to equalize ... @14:31:06.73 SS.reconfigure :exhale @14:31:06.77 SS.hold @14:31:17.79 Intake.select :close @14:31:17.92 SSV.dial :exhaust @14:31:19.16 Exhaust.select :open @14:31:19.26 SS.seek 0ml @14:31:28.31 Exhaust.select :close @14:31:28.42 SSV.dial CBV @14:31:28.47
CBV.dial SSV @14:31:29.17 Allowing 130s for 25.0ml of remaining 600.0ml @14:31:29.18 Intake.select :open @14:31:31.34 Intake @16.3psia, vacuum from 10.0 to 13.0psi @14:31:31.36 SS.reconfigure :inhaling 0.78ml/s @14:31:31.40 SS.seek 25ml @14:31:53.85 Vacuum too high -- waiting up to 40 seconds @14:31:53.86 SS.reconfigure :exhale @14:31:53.90 SS.hold @14:31:58.80 Intake @15.6psia, vacuum from 10.0 to 13.0psi @14:31:58.81 SS.reconfigure :inhaling 0.70ml/s @14:31:58.86 SS.seek 25ml @14:32:12.33 Stroke completed -- waiting for pressure to equalize ... @14:32:12.34 SS.reconfigure :exhale @14:32:12.38 SS.hold @14:32:22.13 Intake.select :close @14:32:22.26 SSV.dial :exhaust @14:32:23.00 Exhaust.select :open @14:32:23.11 SS.seek 0ml @14:32:31.93 Exhaust.select :close @14:32:32.04 SSV.dial CBV @14:32:32.06
CBV.dial SSV @14:32:32.81 Allowing 130s for 25.0ml of remaining 575.0ml @14:32:32.82 Intake.select :open @14:32:34.98 Intake @16.2psia, vacuum from 10.0 to 13.0psi @14:32:35.00 SS.reconfigure :inhaling 0.70ml/s @14:32:35.04 SS.seek 25ml @14:33:05.72 Vacuum too high -- waiting up to 40 seconds @14:33:05.73 SS.reconfigure :exhale @14:33:05.77 SS.hold @14:33:10.63 Intake @15.4psia, vacuum from 9.9 to 12.9psi @14:33:10.64 SS.reconfigure :inhaling 0.63ml/s @14:33:10.69 SS.seek 25ml @14:33:19.31 Stroke completed -- waiting for pressure to equalize ... @14:33:19.32 SS.reconfigure :exhale @14:33:19.36 SS.hold @14:33:27.91 Intake.select :close @14:33:28.05 SSV.dial :exhaust @14:33:29.48 Exhaust.select :open @14:33:29.59 SS.seek 0ml @14:33:38.40 Exhaust.select :close @14:33:38.50 SSV.dial CBV @14:33:38.54
CBV.dial SSV @14:33:39.26 Allowing 130s for 25.0ml of remaining 550.0ml @14:33:39.27 Intake.select :open @14:33:41.44 Intake @16.2psia, vacuum from 10.0 to 13.0psi @14:33:41.45 SS.reconfigure :inhaling 0.63ml/s @14:33:41.50 SS.seek 25ml @14:34:23.81 Stroke completed -- waiting for pressure to equalize ... @14:34:23.82 SS.reconfigure :exhale @14:34:23.86 SS.hold @14:34:36.41 Intake.select :close @14:34:36.54 SSV.dial :exhaust @14:34:37.28 Exhaust.select :open @14:34:37.38 SS.seek 0ml @14:34:46.51 Exhaust.select :close @14:34:46.61 SSV.dial CBV @14:34:46.64
CBV.dial SSV @14:34:47.40 Allowing 130s for 25.0ml of remaining 525.0ml @14:34:47.41 Intake.select :open @14:34:49.57 Intake @16.2psia, vacuum from 10.0 to 13.0psi @14:34:49.58 SS.reconfigure :inhaling 0.63ml/s @14:34:49.62 SS.seek 25ml @14:35:25.00 Vacuum too high -- waiting up to 40 seconds @14:35:25.01 SS.reconfigure :exhale @14:35:25.05 SS.hold @14:35:29.92 Intake @15.1psia, vacuum from 9.6 to 12.6psi @14:35:29.93 SS.reconfigure :inhaling 0.57ml/s @14:35:29.97 SS.seek 25ml @14:35:37.34 Stroke completed -- waiting for pressure to equalize ... @14:35:37.35 SS.reconfigure :exhale @14:35:37.39 SS.hold @14:35:47.03 Intake.select :close @14:35:47.17 SSV.dial :exhaust @14:35:47.92 Exhaust.select :open @14:35:48.03 SS.seek 0ml @14:35:57.24 Exhaust.select :close @14:35:57.35 SSV.dial CBV @14:35:57.40
CBV.dial SSV @14:35:58.11 Allowing 130s for 25.0ml of remaining 500.0ml @14:35:58.12 Intake.select :open @14:36:00.27 Intake @16.1psia, vacuum from 10.0 to 13.0psi @14:36:00.29 SS.reconfigure :inhaling 0.57ml/s @14:36:00.33 SS.seek 25ml @14:36:46.19 Stroke completed -- waiting for pressure to equalize ... @14:36:46.20 SS.reconfigure :exhale @14:36:46.24 SS.hold @14:37:00.48 Intake.select :close @14:37:00.61 SSV.dial :exhaust @14:37:01.35 Exhaust.select :open @14:37:01.46 SS.seek 0ml @14:37:10.32 Exhaust.select :close @14:37:10.42 SSV.dial CBV @14:37:10.44
CBV.dial SSV @14:37:11.20 Allowing 130s for 25.0ml of remaining 475.0ml @14:37:11.21 Intake.select :open @14:37:13.36 Intake @16.2psia, vacuum from 10.0 to 13.0psi @14:37:13.38 SS.reconfigure :inhaling 0.57ml/s @14:37:13.42 SS.seek 25ml @14:37:44.15 Vacuum too high -- waiting up to 40 seconds @14:37:44.16 SS.reconfigure :exhale @14:37:44.21 SS.hold @14:37:50.08 Intake @15.9psia, vacuum from 10.0 to 13.0psi @14:37:50.10 SS.reconfigure :inhaling 0.51ml/s @14:37:50.14 SS.seek 25ml @14:38:08.25 Stroke completed -- waiting for pressure to equalize ... @14:38:08.26 SS.reconfigure :exhale @14:38:08.69 SS.hold @14:38:19.88 Intake.select :close @14:38:20.02 SSV.dial :exhaust @14:38:20.76 Exhaust.select :open @14:38:20.87 SS.seek 0ml @14:38:29.74 Exhaust.select :close @14:38:29.85 SSV.dial CBV @14:38:29.86
CBV.dial SSV @14:38:30.62 Allowing 130s for 25.0ml of remaining 450.0ml @14:38:30.63 Intake.select :open @14:38:32.78 Intake @16.2psia, vacuum from 10.0 to 13.0psi @14:38:32.80 SS.reconfigure :inhaling 0.51ml/s @14:38:32.84 SS.seek 25ml @14:39:17.25 Vacuum too high -- waiting up to 40 seconds @14:39:17.26 SS.reconfigure :exhale @14:39:17.30 SS.hold @14:39:24.65 Intake @16.0psia, vacuum from 10.0 to 13.0psi @14:39:24.67 SS.reconfigure :inhaling 0.47ml/s @14:39:24.71 SS.seek 25ml @14:39:33.18 Stroke completed -- waiting for pressure to equalize ... @14:39:33.19 SS.reconfigure :exhale @14:39:33.23 SS.hold @14:39:44.32 Intake.select :close @14:39:44.45 SSV.dial :exhaust @14:39:45.19 Exhaust.select :open @14:39:45.30 SS.seek 0ml @14:39:54.11 Exhaust.select :close @14:39:54.22 SSV.dial CBV @14:39:54.27
CBV.dial SSV @14:39:54.97 Allowing 130s for 25.0ml of remaining 425.0ml @14:39:54.98 Intake.select :open @14:39:57.14 Intake @16.2psia, vacuum from 10.0 to 13.0psi @14:39:57.16 SS.reconfigure :inhaling 0.47ml/s @14:39:57.20 SS.seek 25ml @14:40:54.42 Stroke completed -- waiting for pressure to equalize ... @14:40:54.43 SS.reconfigure :exhale @14:40:54.47 SS.hold @14:41:10.67 Intake.select :close @14:41:10.81 SSV.dial :exhaust @14:41:11.55 Exhaust.select :open @14:41:11.66 SS.seek 0ml @14:41:20.72 Exhaust.select :close @14:41:20.82 SSV.dial CBV @14:41:20.85
CBV.dial SSV @14:41:21.60 Allowing 130s for 25.0ml of remaining 400.0ml @14:41:21.61 Intake.select :open @14:41:23.76 Intake @16.3psia, vacuum from 10.0 to 13.0psi @14:41:23.78 SS.reconfigure :inhaling 0.47ml/s @14:41:23.82 SS.seek 25ml @14:42:08.31 Vacuum too high -- waiting up to 40 seconds @14:42:08.32 SS.reconfigure :exhale @14:42:08.82 SS.hold @14:42:15.31 Intake @15.9psia, vacuum from 10.0 to 13.0psi @14:42:15.33 SS.reconfigure :inhaling 0.43ml/s @14:42:15.37 SS.seek 25ml @14:42:29.73 Stroke completed -- waiting for pressure to equalize ... @14:42:29.74 SS.reconfigure :exhale @14:42:29.79 SS.hold @14:42:44.14 Intake.select :close @14:42:44.28 SSV.dial :exhaust @14:42:45.02 Exhaust.select :open @14:42:45.12 SS.seek 0ml @14:42:54.25 Exhaust.select :close @14:42:54.35 SSV.dial CBV @14:42:54.37
CBV.dial SSV @14:42:55.13 Allowing 130s for 25.0ml of remaining 375.0ml @14:42:55.14 Intake.select :open @14:42:57.29 Intake @16.2psia, vacuum from 10.0 to 13.0psi @14:42:57.30 SS.reconfigure :inhaling 0.43ml/s @14:42:57.35 SS.seek 25ml @14:44:02.69 Stroke completed -- waiting for pressure to equalize ... @14:44:02.70 SS.reconfigure :exhale @14:44:02.74 SS.hold @14:44:21.83 Intake.select :close @14:44:21.96 SSV.dial :exhaust @14:44:22.70 Exhaust.select :open @14:44:22.81 SS.seek 0ml @14:44:31.84 Exhaust.select :close @14:44:31.95 SSV.dial CBV @14:44:31.98
CBV.dial SSV @14:44:32.72 Allowing 130s for 25.0ml of remaining 350.0ml @14:44:32.73 Intake.select :open @14:44:34.89 Intake @16.2psia, vacuum from 10.0 to 13.0psi @14:44:34.91 SS.reconfigure :inhaling 0.43ml/s @14:44:34.95 SS.seek 25ml @14:45:41.07 Stroke completed -- waiting for pressure to equalize ... @14:45:41.08 SS.reconfigure :exhale @14:45:41.12 SS.hold @14:46:01.44 Intake.select :close @14:46:01.57 SSV.dial :exhaust @14:46:02.31 Exhaust.select :open @14:46:02.42 SS.seek 0ml @14:46:11.29 Exhaust.select :close @14:46:11.40 SSV.dial CBV @14:46:11.92
CBV.dial SSV @14:46:12.64 Allowing 130s for 25.0ml of remaining 325.0ml @14:46:12.65 Intake.select :open @14:46:14.80 Intake @16.2psia, vacuum from 10.0 to 13.0psi @14:46:14.82 SS.reconfigure :inhaling 0.43ml/s @14:46:14.86 SS.seek 25ml @14:47:07.99 Vacuum too high -- waiting up to 40 seconds @14:47:08.00 SS.reconfigure :exhale @14:47:08.04 SS.hold @14:47:17.70 Intake @16.4psia, vacuum from 10.0 to 13.0psi @14:47:17.71 SS.reconfigure :inhaling 0.38ml/s @14:47:17.75 SS.seek 25ml @14:47:32.93 Stroke completed -- waiting for pressure to equalize ... @14:47:32.94 SS.reconfigure :exhale @14:47:32.98 SS.hold @14:47:49.25 Intake.select :close @14:47:49.39 SSV.dial :exhaust @14:47:50.15 Exhaust.select :open @14:47:50.26 SS.seek 0ml @14:47:59.07 Exhaust.select :close @14:47:59.17 SSV.dial CBV @14:47:59.20
CBV.dial SSV @14:47:59.95 Allowing 130s for 25.0ml of remaining 300.0ml @14:47:59.96 Intake.select :open @14:48:02.11 Intake @16.3psia, vacuum from 10.0 to 13.0psi @14:48:02.13 SS.reconfigure :inhaling 0.38ml/s @14:48:02.17 SS.seek 25ml @14:49:21.85 Stroke completed -- waiting for pressure to equalize ... @14:49:21.86 SS.reconfigure :exhale @14:49:21.90 SS.hold @14:49:44.30 Intake.select :close @14:49:44.43 SSV.dial :exhaust @14:49:45.18 Exhaust.select :open @14:49:45.29 SS.seek 0ml @14:49:54.18 Exhaust.select :close @14:49:54.28 SSV.dial CBV @14:49:54.30
CBV.dial SSV @14:49:55.06 Allowing 130s for 25.0ml of remaining 275.0ml @14:49:55.07 Intake.select :open @14:49:57.23 Intake @16.3psia, vacuum from 10.0 to 13.0psi @14:49:57.25 SS.reconfigure :inhaling 0.38ml/s @14:49:57.29 SS.seek 25ml @14:51:17.96 Stroke completed -- waiting for pressure to equalize ... @14:51:17.97 SS.reconfigure :exhale @14:51:18.01 SS.hold @14:51:40.77 Intake.select :close @14:51:40.91 SSV.dial :exhaust @14:51:41.66 Exhaust.select :open @14:51:41.77 SS.seek 0ml @14:51:50.65 Exhaust.select :close @14:51:50.75 SSV.dial CBV @14:51:50.77
CBV.dial SSV @14:51:51.54 Allowing 130s for 25.0ml of remaining 250.0ml @14:51:51.55 Intake.select :open @14:51:53.70 Intake @16.2psia, vacuum from 10.0 to 13.0psi @14:51:53.72 SS.reconfigure :inhaling 0.38ml/s @14:51:53.76 SS.seek 25ml @14:53:05.42 Vacuum too high -- waiting up to 40 seconds @14:53:05.43 SS.reconfigure :exhale @14:53:05.47 SS.hold @14:53:18.02 Intake @16.4psia, vacuum from 10.0 to 13.0psi @14:53:18.04 SS.reconfigure :inhaling 0.34ml/s @14:53:18.08 SS.seek 25ml @14:53:27.50 Stroke completed -- waiting for pressure to equalize ... @14:53:27.51 SS.reconfigure :exhale @14:53:27.55 SS.hold @14:53:43.82 Intake.select :close @14:53:43.95 SSV.dial :exhaust @14:53:44.69 Exhaust.select :open @14:53:44.80 SS.seek 0ml @14:53:53.67 Exhaust.select :close @14:53:53.78 SSV.dial CBV @14:53:53.81
CBV.dial SSV @14:53:54.55 Allowing 130s for 25.0ml of remaining 225.0ml @14:53:54.56 Intake.select :open @14:53:56.71 Intake @16.3psia, vacuum from 10.0 to 13.0psi @14:53:56.73 SS.reconfigure :inhaling 0.34ml/s @14:53:56.77 SS.seek 25ml @14:55:30.59 Stroke completed -- waiting for pressure to equalize ... @14:55:30.60 SS.reconfigure :exhale @14:55:30.64 SS.hold @14:55:57.03 Intake.select :close @14:55:57.17 SSV.dial :exhaust @14:55:57.93 Exhaust.select :open @14:55:58.04 SS.seek 0ml @14:56:06.97 Exhaust.select :close @14:56:07.07 SSV.dial CBV @14:56:07.09
CBV.dial SSV @14:56:07.85 Allowing 130s for 25.0ml of remaining 200.0ml @14:56:07.86 Intake.select :open @14:56:10.01 Intake @16.5psia, vacuum from 10.0 to 13.0psi @14:56:10.02 SS.reconfigure :inhaling 0.34ml/s @14:56:10.06 SS.seek 25ml @14:57:26.10 Vacuum too high -- waiting up to 40 seconds @14:57:26.11 SS.reconfigure :exhale @14:57:26.64 SS.hold @14:57:37.59 Intake @16.3psia, vacuum from 10.0 to 13.0psi @14:57:37.61 SS.reconfigure :inhaling 0.31ml/s @14:57:37.65 SS.seek 25ml @14:57:56.34 Stroke completed -- waiting for pressure to equalize ... @14:57:56.35 SS.reconfigure :exhale @14:57:56.39 SS.hold @14:58:17.86 SS.reconfigure :default @14:58:17.91 SS.hold @14:58:18.02 Intake.select :close @14:58:18.16 ShallowSampler::Clogged in MAIN -- Filtering too Slowly after 825.1ml @14:58:18.17 SSV.dial :exhaust @14:58:18.90 Exhaust.select :open @14:58:19.01 SS.reconfigure :exhale @14:58:19.05 SS.seek 0ml @14:58:27.95 Exhaust.select :close @14:58:28.05 Sampled 825.1ml @14:58:28.06 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @14:58:28.07 Intake.select :close @14:58:28.13 CSV.dial :waste @14:58:28.20 CS.seek 0ml @14:58:28.27 CSV.dial :air @14:58:28.30 SSV.dial CBV,avoiding: :exhaust @14:58:28.37
CBV.dial SSV @14:58:29.06 CS.seek 10ml @14:58:30.19
CTV.dial :air @14:58:30.96 delay 15 seconds @14:58:45.98 SSV.dial :spit,avoiding: :exhaust @14:58:47.29 CTV.dial CSV,avoiding: :intake @14:58:47.31
SS.seek 0ml @14:58:47.43 SSV.dial CBV,avoiding: :exhaust @14:58:53.56 CBV.dial between SSV and waste2 @14:58:53.95 CTV.dial CSV,avoiding: :air @14:58:53.97
CSV.dial CTV @14:58:54.53 CS.reconfigure :pressurize @14:58:54.57 CS.seek 0ml @14:59:13.26 BubblePoint: Intake @39.4psia, Outlet @15.2psia @14:59:13.27 CBV.dial SSV @14:59:13.59 SS.reconfigure :default @14:59:13.63 SS.seek 10ml @14:59:19.31 delay 15 seconds @14:59:34.42 Evacuated: Intake @17.9psia, Outlet @9.8psia @14:59:34.43 SSV.dial :spit,avoiding: :exhaust @14:59:35.74 SS.reconfigure :exhale @14:59:35.78 SS.seek 0ml @14:59:41.35 SS.reconfigure :fast @14:59:41.39 SS.seek 5ml @14:59:44.30 SSV.dial CBV,avoiding: :exhaust @14:59:44.32
CBV.dial SSV @14:59:45.64 CTV.dial :air,avoiding: :intake @14:59:46.98 CSV.dial CTV @14:59:47.01
SS.seek 10ml @14:59:48.00 CSV.dial :air @14:59:48.43 CS.reconfigure :fast @14:59:48.48 CS.seek 0ml @14:59:49.48
SSV.dial :spit,avoiding: :exhaust @14:59:50.80 SS.reconfigure :exhale @14:59:50.84 SS.seek 0ml @14:59:55.81 CS.stop @14:59:56.18
SS.reconfigure :fast @14:59:56.22 SS.seek 5ml @14:59:59.05 SSV.dial CBV,avoiding: :exhaust @14:59:59.07
CBV.dial SSV @15:00:01.77 SSV.dial :spit,avoiding: :exhaust @15:00:03.14 SS.reconfigure :default @15:00:03.19 SS.seek 0ml @15:00:06.57 SS.stop @15:00:06.61 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @15:00:06.62 Intake.select :close @15:00:06.69 CSV.dial :waste @15:00:06.81 SSV.dial CBV,avoiding: :exhaust @15:00:06.82
CBV.dial SSV @15:00:07.45 CS.seek 0ml @15:00:07.51 CSV.dial :air @15:00:08.17
CTV.dial :air @15:00:08.18 delay 15 seconds @15:00:08.25 CS.seek 10ml @15:00:23.20
SSV.dial :spit,avoiding: :exhaust @15:00:24.52 CTV.dial CSV,avoiding: :intake @15:00:24.55
SS.reconfigure :exhale @15:00:24.64 SS.seek 0ml @15:00:25.03 SSV.dial CBV,avoiding: :exhaust @15:00:32.62 CBV.dial between SSV and waste2 @15:00:33.01 CTV.dial CSV,avoiding: :air @15:00:33.03
CSV.dial CTV @15:00:33.47 CS.reconfigure :pressurize @15:00:33.52 CS.seek 0ml @15:00:54.41 BubblePoint: Intake @38.3psia, Outlet @14.1psia @15:00:54.42 CBV.dial SSV @15:00:54.74 SS.reconfigure :default @15:00:54.79 SS.seek 10ml @15:01:00.56 delay 15 seconds @15:01:15.67 Evacuated: Intake @19.2psia, Outlet @11.3psia @15:01:15.68 SSV.dial :spit,avoiding: :exhaust @15:01:17.00 SS.reconfigure :exhale @15:01:17.04 SS.seek 0ml @15:01:22.27 SS.reconfigure :fast @15:01:22.31 SS.seek 5ml @15:01:25.14 SSV.dial CBV,avoiding: :exhaust @15:01:25.16
CBV.dial SSV @15:01:26.48 CTV.dial :air,avoiding: :intake @15:01:27.82 CSV.dial CTV @15:01:27.86
SS.seek 10ml @15:01:28.85 CSV.dial :air @15:01:29.31 CS.reconfigure :fast @15:01:29.35 CS.seek 0ml @15:01:30.34
SSV.dial :spit,avoiding: :exhaust @15:01:31.66 SS.reconfigure :exhale @15:01:31.70 SS.seek 0ml @15:01:34.78 CS.stop @15:01:37.10
SS.reconfigure :fast @15:01:37.14 SS.seek 5ml @15:01:40.07 SSV.dial CBV,avoiding: :exhaust @15:01:40.09
CBV.dial SSV @15:01:42.41 SSV.dial :spit,avoiding: :exhaust @15:01:43.72 SS.reconfigure :default @15:01:43.76 SS.seek 0ml @15:01:47.02 SS.stop @15:01:47.06 Lysing: Adding 2.0ml AqMeOH @15:01:47.07 CBV.dial between CSV and waste1,avoiding: :DBV @15:01:48.54 CTV.dial CSV @15:01:49.85 CSV.dial CSR @15:01:50.63 Pulling 1ml of CSR.AqMeOH @15:01:50.64 CSR.select :AqMeOH @15:01:50.75 CS.reconfigure :default @15:01:50.79 CS.seek 1ml @15:01:54.35 delay 5 seconds @15:01:59.37 CSR.select :air @15:01:59.52 CS.seek 6ml @15:02:13.22 delay 2 seconds @15:02:15.24 CSV.dial :waste2 @15:02:15.67 CS.reconfigure :fast @15:02:15.71 CS.seek 0ml @15:02:29.94 CSV.dial CSR @15:02:30.45 Pulling 2ml of CSR.AqMeOH @15:02:30.46 CSR.select :AqMeOH @15:02:30.57 CS.reconfigure :default @15:02:30.61 CS.seek 2ml @15:02:36.51 delay 5 seconds @15:02:41.52 CSR.select :air @15:02:41.67 CS.seek 5ml @15:02:50.35 delay 2 seconds @15:02:52.36 CSV.dial CTV @15:02:52.79 CS.seek 1ml @15:03:03.95 Before lysing Collection Puck Heater at 21.6C/39.5psia @15:03:03.97 Heating Puck.from 4, :sh1 in Collection Clamp to 70C for 10:00 @15:03:03.98 CH.seek 70C,8:00 @15:04:52.02 CH.hold 10:00,70C @15:04:52.19 Begun lysing Collection Puck Heater at 68C/46.7psia @15:14:52.21 Ended lysing Collection Puck Heater at 70C/44.5psia @15:14:52.22 CS.seek 0ml @15:14:55.84 CTV.dial between spare and air,avoiding: :intake @15:14:56.87 CSV.dial :air @15:14:57.32 CS.seek 5ml @15:15:10.89 CSV.dial CTV @15:15:11.34 delay 2 seconds @15:15:13.35 CSV.dial :air @15:15:13.80 delay 2 seconds @15:15:15.82 CSV.dial CTV @15:15:16.27 delay 2 seconds @15:15:18.29 CSV.dial :air @15:15:18.74 delay 2 seconds @15:15:20.76 CS.seek 0ml @15:15:34.38 Puck @73.0psia after final lysis pressurization @15:15:34.40 Recovering Lysate into Collection syringe @15:15:34.41 CSV.dial CBV @15:15:35.40 CBV.dial CSV @15:15:35.77 CS.seek 5ml @15:15:49.43 delay 5 seconds @15:15:54.44 CTV.dial :air @15:15:54.97 CS.seek 7.001ml @15:16:00.93 delay 5 seconds @15:16:05.94 CSV.dial :air2 @15:16:06.39 CS.seek 10ml @15:16:14.83 CSV.dial between air and CTV,avoiding: CBV @15:16:15.98 Lysing Complete @15:16:16.05 Storing sh1 puck in Collection Clamp to tube 3 @15:16:16.21 SC.reconfigure :tight @15:16:16.25 SC.seek 3 @15:16:19.85 SC.configure :limp @15:16:20.04 Elbow.reconfigure :tight @15:16:20.08 Elbow.seek 3 @15:16:21.86 Forearm.reconfigure :tight @15:16:21.91 Forearm.seek 3 + 450 counts @15:16:22.92 SE.reconfigure :pick @15:16:22.97 SE.seek :beamBreak @15:16:24.48 SE.configure :default @15:16:24.63 Elbow.reconfigure :limp @15:16:24.67 Forearm.reconfigure :loose @15:16:24.71 Forearm.seek 3 @15:16:24.91 Elbow.hold @15:16:24.98 Elbow.reconfigure :tight @15:16:25.07 SE.seek 25.1 Pucks below Top Plate @15:16:25.61 Forearm.force -20 @15:16:25.64 Forearm.seek 3 + 313 counts @15:16:25.91 Forearm.reconfigure :tight @15:16:25.96 Forearm.seek 3 + 450 counts @15:16:26.14 SE.seek 26.1 Pucks below Top Plate @15:16:27.15 Forearm.reconfigure :loose @15:16:27.21 Forearm.seek :retract @15:16:28.16 Elbow.seek CC @15:16:29.81 Elbow.reconfigure :loose @15:16:29.90 Forearm.seek CC @15:16:30.88 Hand.close @15:16:31.46 CC.seek :open @15:16:36.17 Forearm.seek :retract @15:16:37.13 Elbow.reconfigure :tight @15:16:37.17 Elbow.seek 3 @15:16:38.94 Elbow.reconfigure :limp @15:16:38.99 Forearm.seek 3 @15:16:39.96 Elbow.hold @15:16:40.02 Elbow.reconfigure :tight @15:16:40.06 Hand.open @15:16:40.82 Forearm.force -20 @15:16:40.85 Forearm.seek 3 + 310 counts @15:16:41.15 Forearm.reconfigure :tight @15:16:41.19 Forearm.seek 3 + 450 counts @15:16:41.46 SE.seek :down @15:16:42.39 SE.stop @15:16:42.58
Forearm.reconfigure :loose @15:16:42.62 Forearm.seek :retract @15:16:43.54 Tranferring DA @15:16:43.55 Beginning samplePassC @15:16:43.56 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @15:16:43.74 Elbow.seek FlushPuck::Garage @15:16:45.56 Elbow.reconfigure :loose @15:16:45.65 Forearm.seek :garage @15:16:46.61 Hand.close @15:16:47.32 Forearm.seek :retract @15:16:48.27 Elbow.reconfigure :tight @15:16:48.32 Elbow.seek CC @15:16:51.23 Elbow.reconfigure :loose @15:16:51.32 Forearm.reconfigure :tight @15:16:51.36 Forearm.seek CC @15:16:52.62 CC.seek :closed @15:16:57.14 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.32 @15:16:57.21 Hand.open @15:16:57.89 Forearm.reconfigure :loose @15:16:57.93 Forearm.seek :retract @15:16:58.89 PSV.dial PSR @15:17:00.25 Pulling 1.5ml of PSR.flush @15:17:00.26 PSR.select :flush @15:17:00.38 PS.seek 1.5ml @15:17:16.47 delay 5 seconds @15:17:21.48 PSV.dial :waste1 @15:17:21.94 PS.seek 0ml @15:17:38.09 PSV.dial PSR @15:17:38.56 PSR.select :air @15:17:38.67 PS.seek 2.5ml @15:18:05.06 PRV.dial CBV,avoiding: :ab2 @15:18:05.08
CBV.dial PRV @15:18:06.74 PSV.dial :mixloop @15:18:07.76 CTV.dial :air,avoiding: :intake @15:18:07.77 PTV.dial :PRVmixing @15:18:09.08 PS.seek 0ml @15:18:35.46 PSV.dial :air @15:18:35.92 PS.seek 2.5ml @15:19:02.24 PSV.dial :mixloop @15:19:02.70 PS.seek 0ml @15:19:29.05 PSV.dial :air @15:19:29.51 PS.seek 2.5ml @15:19:55.80 PSV.dial :mixloop @15:19:56.26 PS.seek 0ml @15:20:23.31 Garaging FlushPuck in Collection Clamp @15:20:23.38 Elbow.reconfigure :tight @15:20:23.42 Elbow.seek CC @15:20:23.79 Elbow.reconfigure :loose @15:20:23.88 Forearm.seek CC @15:20:24.91 Hand.close @15:20:25.46 CC.seek :open @15:20:30.07 Forearm.seek :retract @15:20:31.03 Elbow.reconfigure :tight @15:20:31.08 Elbow.seek :garage @15:20:34.00 Elbow.reconfigure :loose @15:20:34.08 Forearm.reconfigure :tight @15:20:34.13 Forearm.seek :garage @15:20:35.08 Hand.open @15:20:35.76 Forearm.reconfigure :loose @15:20:35.80 Forearm.seek :retract @15:20:36.78 PSV.dial :mixloop @15:20:36.79 PTV.dial :PRVmixing @15:20:36.81 PRV.dial CBV @15:20:36.83
CBV.dial PRV @15:20:36.85 CTV.dial :air,avoiding: :intake @15:20:36.86 Loading lyfil puck from tube 4 into Collection Clamp @15:20:37.08 SC.reconfigure :tight @15:20:37.13 SC.seek 4 @15:20:40.67 SC.configure :limp @15:20:40.86 Elbow.reconfigure :tight @15:20:40.90 Elbow.seek 4 @15:20:42.81 Forearm.reconfigure :tight @15:20:42.86 Forearm.seek 4 + 450 counts @15:20:43.84 SE.reconfigure :pick @15:20:43.89 SE.seek :beamBreak @15:20:46.71 SE.configure :default @15:20:46.85 Elbow.seek 4 @15:20:47.02 Forearm.seek 4 + 300 counts @15:20:47.30 Forearm.reconfigure :loose @15:20:47.34 Hand.close @15:20:47.97 SE.seek :down @15:20:48.16
Forearm.seek :retract @15:20:49.10 Elbow.seek CC @15:20:50.40 SE.stop @15:20:50.73
Elbow.reconfigure :loose @15:20:50.82 Forearm.reconfigure :tight @15:20:50.86 Forearm.seek CC @15:20:52.05 CC.seek :closed @15:20:56.56 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.10 @15:20:56.57 Hand.open @15:20:57.27 Forearm.reconfigure :loose @15:20:57.31 Forearm.seek :retract @15:20:58.27 CTV.dial CSV,avoiding: :intake @15:20:59.61 CSV.dial CTV @15:20:59.91 PS.seek 2.5ml @15:21:26.33 CS.seek 4.5ml @15:21:41.23 delay 8 seconds @15:21:49.26 PSV.dial :air @15:21:49.72 delay 2 seconds @15:21:51.74 PSV.dial :waste2 @15:21:52.47 PS.seek 0ml @15:22:18.86 CBV.dial between PRV and waste1 @15:22:19.19 CSV.dial :air @15:22:19.65 CS.seek 10ml @15:22:34.61 CSV.dial CTV @15:22:35.05 CS.seek 0ml @15:23:01.67 CBV.dial :waste1 @15:23:01.97 delay 5 seconds @15:23:06.99 CTV.dial :air,avoiding: :intake @15:23:08.87 Storing lyfil puck in Collection Clamp to tube 3 @15:23:09.03 SC.reconfigure :tight @15:23:09.07 SC.seek 3 @15:23:12.66 SC.configure :limp @15:23:12.86 Elbow.reconfigure :tight @15:23:12.90 Elbow.seek 3 @15:23:14.66 Forearm.reconfigure :tight @15:23:14.70 Forearm.seek 3 + 450 counts @15:23:15.78 SE.reconfigure :pick @15:23:15.83 SE.seek :beamBreak @15:23:17.21 SE.configure :default @15:23:17.36 Elbow.reconfigure :limp @15:23:17.40 Forearm.reconfigure :loose @15:23:17.44 Forearm.seek 3 @15:23:17.61 Elbow.hold @15:23:17.67 Elbow.reconfigure :tight @15:23:17.76 SE.seek 26.11 Pucks below Top Plate @15:23:18.21 Forearm.force -20 @15:23:18.24 Forearm.seek 3 + 313 counts @15:23:18.52 Forearm.reconfigure :tight @15:23:18.56 Forearm.seek 3 + 450 counts @15:23:18.79 SE.seek 27.11 Pucks below Top Plate @15:23:19.34 Forearm.reconfigure :loose @15:23:19.38 Forearm.seek :retract @15:23:20.30 Elbow.seek CC @15:23:21.93 Elbow.reconfigure :loose @15:23:22.02 Forearm.seek CC @15:23:22.96 Hand.close @15:23:23.58 CC.seek :open @15:23:28.29 Forearm.seek :retract @15:23:29.27 Elbow.reconfigure :tight @15:23:29.31 Elbow.seek 3 @15:23:31.08 Elbow.reconfigure :limp @15:23:31.12 Forearm.seek 3 @15:23:32.14 Elbow.hold @15:23:32.20 Elbow.reconfigure :tight @15:23:32.24 Hand.open @15:23:32.94 Forearm.force -20 @15:23:32.97 Forearm.seek 3 + 310 counts @15:23:33.28 Forearm.reconfigure :tight @15:23:33.32 Forearm.seek 3 + 450 counts @15:23:33.61 SE.seek :down @15:23:34.47 SE.stop @15:23:34.73
Forearm.reconfigure :loose @15:23:34.77 Forearm.seek :retract @15:23:35.72 PSV.dial :air @15:23:36.46 PS.seek 2.5ml @15:24:02.77 delay 1 second @15:24:03.78 PSV.dial :mixloop @15:24:04.21 delay 1 second @15:24:05.22 PSV.dial :air @15:24:05.78 delay 1 second @15:24:06.80 PSV.dial :mixloop @15:24:07.24 delay 1 second @15:24:08.25 PSV.dial :air @15:24:08.72 delay 1 second @15:24:09.74 PS.seek 0ml @15:24:36.11 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @15:24:36.28 Elbow.seek FlushPuck::Garage @15:24:38.02 Elbow.reconfigure :loose @15:24:38.11 Forearm.seek :garage @15:24:39.12 Hand.close @15:24:39.81 Forearm.seek :retract @15:24:40.75 Elbow.reconfigure :tight @15:24:40.79 Elbow.seek PC @15:24:42.04 Elbow.reconfigure :loose @15:24:42.12 Forearm.reconfigure :tight @15:24:42.81 Forearm.seek PC @15:24:44.07 PC.seek :closed @15:24:48.67 Processing Clamp onSpring/offSpring for 16/26 samples, on/off torque 1.55 @15:24:48.74 Hand.open @15:24:49.38 Forearm.reconfigure :loose @15:24:49.43 Forearm.seek :retract @15:24:50.35 PRV.dial :air @15:24:50.80 PSV.dial :mixloop @15:24:51.33 PS.seek 0.3ml @15:24:55.17 PSV.dial PSR @15:24:56.26 Pulling 0.4ml of PSR.air PSR.select :air @15:24:56.29 PS.seek 0.7ml @15:25:01.14 delay 3 seconds @15:25:04.16 PSR.select :air @15:25:04.18 PS.seek 2.5ml @15:25:23.39 delay 3 seconds @15:25:26.41 PSV.dial :mixloop @15:25:27.44 PTV.dial :mixing @15:25:28.19 PBV.dial :waste2 @15:25:29.12 PS.seek 0ml @15:25:55.42 Garaging FlushPuck in Processing Clamp @15:25:55.50 Elbow.reconfigure :tight @15:25:55.54 Elbow.seek PC @15:25:55.86 Elbow.reconfigure :loose @15:25:55.95 Forearm.seek PC @15:25:56.90 Hand.close @15:25:57.46 PC.seek :open @15:26:02.15 Forearm.seek :retract @15:26:03.17 Elbow.reconfigure :tight @15:26:03.21 Elbow.seek :garage @15:26:04.49 Elbow.reconfigure :loose @15:26:04.58 Forearm.reconfigure :tight @15:26:04.62 Forearm.seek :garage @15:26:05.57 Hand.open @15:26:06.26 Forearm.reconfigure :loose @15:26:06.30 Forearm.seek :retract @15:26:07.26 Loading da puck from tube 4 into Processing Clamp @15:26:07.47 SC.reconfigure :tight @15:26:07.51 SC.seek 4 @15:26:11.14 SC.configure :limp @15:26:11.33 Elbow.reconfigure :tight @15:26:11.38 Elbow.seek 4 @15:26:13.20 Forearm.reconfigure :tight @15:26:13.25 Forearm.seek 4 + 450 counts @15:26:14.32 SE.reconfigure :pick @15:26:14.37 SE.seek :beamBreak @15:26:17.26 SE.configure :default @15:26:17.39 Elbow.seek 4 @15:26:17.56 Forearm.seek 4 + 300 counts @15:26:18.00 Forearm.reconfigure :loose @15:26:18.04 Hand.close @15:26:18.71 SE.seek :down @15:26:18.90
Forearm.seek :retract @15:26:19.84 Elbow.seek PC @15:26:21.19 SE.stop @15:26:22.38
Elbow.reconfigure :loose @15:26:22.47 Forearm.reconfigure :tight @15:26:22.51 Forearm.seek PC @15:26:23.70 PC.seek :closed @15:26:28.57 Processing Clamp onSpring/offSpring for 13/26 samples, on/off torque 1.56 @15:26:28.58 Hand.open @15:26:29.26 Forearm.reconfigure :loose @15:26:29.30 Forearm.seek :retract @15:26:30.26 Heating Puck.from 4, :da in Processing Clamp to 28C for 2:00:00 @15:26:30.27 PH.seek 28C,5:00 @15:26:58.63 PH.hold 2:00:00,28C @15:26:58.69 delay 1:30 @15:28:28.72 PSR.select :air @15:28:28.73 PSV.dial PSR @15:28:29.82 Pulling 0.5ml of PSR.wash PSR.select :wash @15:28:29.94 PS.seek 0.5ml @15:28:35.83 delay 5 seconds @15:28:40.84 PSR.select :air @15:28:40.96 PS.seek 2ml @15:28:57.04 PTV.dial :puck @15:28:57.74 PSV.dial PTV @15:28:58.50 PBV.dial :waste1 @15:28:59.87 PS.seek 1.5ml @15:29:05.79 delay 3 seconds @15:29:08.81 PBV.dial between PSV and waste1 @15:29:09.18 PS.seek 0ml @15:29:25.29 delay 5:00 @15:34:25.31 recoverPSRreagent08 @15:34:25.32 PTV.dial :puck @15:34:25.33 PBV.dial :waste1 @15:34:25.69 delay 30 seconds @15:34:55.72 PBV.dial between PSV and waste1 @15:34:56.07 PSR.select :air @15:34:56.08 PSV.dial PSR @15:34:56.84 PS.reconfigure :fast @15:34:56.88 PS.seek 2.5ml @15:35:20.38 PSV.dial PTV @15:35:21.13 delay 5 seconds @15:35:26.15 PSV.dial PSR @15:35:26.89 delay 5 seconds @15:35:31.91 PSV.dial PTV @15:35:32.66 delay 5 seconds @15:35:37.68 PSV.dial :air @15:35:38.43 PS.seek 0ml @15:36:01.44 PTV.dial :PRVmixing,via: PRV @15:36:02.75 PSV.dial :mixloop @15:36:03.22 PRV.dial :ab1 @15:36:03.98 PS.reconfigure :slow @15:36:04.02 PS.seek 0.15ml @15:36:07.63 delay 2 seconds @15:36:09.64 CBV.dial PRV @15:36:09.67
PRV.dial CBV @15:36:10.22 PS.seek 0.35ml @15:36:14.81 delay 2 seconds @15:36:16.83 CBV.dial between PRV and PSV @15:36:17.14 PRV.dial :air @15:36:17.59 PS.seek 0.9ml @15:36:29.28 PRV.dial :waste2 @15:36:29.99 PS.seek 0.5ml @15:36:38.63 addPMLReagent08 @15:36:38.64 PSV.dial :air @15:36:39.10 PS.reconfigure :default @15:36:39.14 PS.seek 2ml @15:36:55.44 PSV.dial :PML @15:36:55.89 PTV.dial :mixing @15:36:56.63 PBV.dial :waste1 @15:36:57.03 PS.seek 1.85ml @15:36:59.47 PBV.dial between PSV and waste1 @15:36:59.84 PS.seek 0ml @15:37:19.56 delay 2 seconds @15:37:21.58 Beginning samplePassC @15:37:21.59 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @15:37:21.75 Elbow.reconfigure :tight @15:37:21.80 Elbow.seek FlushPuck::Garage @15:37:23.13 Elbow.reconfigure :loose @15:37:23.22 Forearm.seek :garage @15:37:24.24 Hand.close @15:37:24.94 Forearm.seek :retract @15:37:25.91 Elbow.reconfigure :tight @15:37:25.95 Elbow.seek CC @15:37:28.76 Elbow.reconfigure :loose @15:37:29.32 Forearm.reconfigure :tight @15:37:29.36 Forearm.seek CC @15:37:30.62 CC.seek :closed @15:37:35.16 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.37 @15:37:35.22 Hand.open @15:37:35.87 Forearm.reconfigure :loose @15:37:35.92 Forearm.seek :retract @15:37:36.88 PSV.dial PSR @15:37:38.09 Pulling 1.5ml of PSR.flush @15:37:38.10 PSR.select :flush @15:37:38.21 PS.seek 1.5ml @15:37:54.44 delay 5 seconds @15:37:59.46 PSV.dial :waste1 @15:37:59.93 PS.seek 0ml @15:38:16.12 PSV.dial PSR @15:38:16.59 PSR.select :air @15:38:16.70 PS.seek 2.5ml @15:38:43.07 PRV.dial CBV,avoiding: :ab2 @15:38:43.09
CBV.dial PRV @15:38:44.69 PSV.dial :mixloop @15:38:45.70 CTV.dial :air,avoiding: :intake @15:38:45.72 PTV.dial :PRVmixing @15:38:46.48 PS.seek 0ml @15:39:12.78 PSV.dial :air @15:39:13.35 PS.seek 2.5ml @15:39:39.71 PSV.dial :mixloop @15:39:40.16 PS.seek 0ml @15:40:06.56 PSV.dial :air @15:40:07.02 PS.seek 2.5ml @15:40:33.40 PSV.dial :mixloop @15:40:33.86 PS.seek 0ml @15:41:00.40 Garaging FlushPuck in Collection Clamp @15:41:00.47 Elbow.reconfigure :tight @15:41:00.51 Elbow.seek CC @15:41:00.63 Elbow.reconfigure :loose @15:41:00.72 Forearm.seek CC @15:41:01.68 Hand.close @15:41:02.31 CC.seek :open @15:41:06.95 Forearm.seek :retract @15:41:07.95 Elbow.reconfigure :tight @15:41:08.00 Elbow.seek :garage @15:41:10.90 Elbow.reconfigure :loose @15:41:10.98 Forearm.reconfigure :tight @15:41:11.03 Forearm.seek :garage @15:41:11.99 Hand.open @15:41:12.62 Forearm.reconfigure :loose @15:41:12.66 Forearm.seek :retract @15:41:13.61 delayUntil "15:45:21.57" @15:41:13.64 Whole Cell Archive sampling 1000ml @15:41:13.65 Loading wcr puck from tube 4 into Collection Clamp @15:41:13.88 SC.reconfigure :tight @15:41:13.92 SC.seek 4 @15:41:14.23 SC.configure :limp @15:41:14.42 Elbow.reconfigure :tight @15:41:14.47 Elbow.seek 4 @15:41:16.35 Forearm.reconfigure :tight @15:41:16.40 Forearm.seek 4 + 450 counts @15:41:17.42 SE.reconfigure :pick @15:41:17.47 SE.seek :beamBreak @15:41:20.48 SE.configure :default @15:41:20.62 Elbow.seek 4 @15:41:20.79 Forearm.seek 4 + 300 counts @15:41:21.03 Forearm.reconfigure :loose @15:41:21.53 Hand.close @15:41:22.20 SE.seek :down @15:41:22.39 Forearm.seek :retract @15:41:23.35 Elbow.seek CC @15:41:24.79 SE.stop @15:41:25.00 Elbow.reconfigure :loose @15:41:25.10 Forearm.reconfigure :tight @15:41:25.14 Forearm.seek CC @15:41:26.33 CC.seek :closed @15:41:30.84 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.24 @15:41:30.85 Hand.open @15:41:31.50 Forearm.reconfigure :loose @15:41:31.54 Forearm.seek :retract @15:41:32.49 collectSample 1000ml w/bubble point of 35psi @15:41:32.50 Priming 7ml @15:41:32.51 Intake.select :close @15:41:32.52 Exhaust.select :close @15:41:32.53 SS.hold @15:41:32.72 Calibrating pressure sensors @15:41:32.73 CTV.dial :air @15:41:32.75 SSV.dial :spit,avoiding: :exhaust @15:41:32.76 SS.reconfigure :fast @15:41:32.81 SS.seek 5ml @15:41:35.48 SSV.dial CBV @15:41:35.50 CBV.dial SSV @15:41:36.85 delay 3.8 seconds @15:41:40.77 SSV.dial :spit @15:41:42.09 SS.seek 0ml @15:41:44.94 SSV.dial CBV @15:41:46.25 CTV.dial :intake @15:41:47.00 SSV.dial CBV @15:41:47.02 CBV.dial SSV @15:41:47.04 Allowing 40s for 7.0ml of remaining 7.0ml @15:41:47.05 Intake.select :open @15:41:49.22 Intake @15.4psia, vacuum from 9.9 to 12.9psi @15:41:49.24 SS.reconfigure :inhaling 2.20ml/s @15:41:49.28 SS.seek 7ml @15:41:56.07 Stroke completed -- waiting for pressure to equalize ... @15:41:56.08 SS.reconfigure :exhale @15:41:56.12 SS.hold @15:42:04.58 Intake.select :close @15:42:04.73 SSV.dial :exhaust @15:42:05.47 Exhaust.select :open @15:42:05.59 SS.seek 0ml @15:42:09.79 Exhaust.select :close @15:42:09.89 Sampling 1000.0ml @15:42:09.90 Intake.select :close @15:42:09.91 Exhaust.select :close @15:42:09.93 SS.reconfigure :default @15:42:09.96 SS.hold @15:42:10.17 CTV.dial :intake @15:42:10.19 SSV.dial CBV @15:42:10.21 CBV.dial SSV @15:42:11.00 Allowing 130s for 25.0ml of remaining 1000.0ml @15:42:11.02 Intake.select :open @15:42:13.18 Intake @15.3psia, vacuum from 9.8 to 12.8psi @15:42:13.20 SS.reconfigure :inhaling 2.20ml/s @15:42:13.24 SS.seek 25ml @15:42:21.92 Vacuum too high -- waiting up to 40 seconds @15:42:21.93 SS.reconfigure :exhale @15:42:21.97 SS.hold @15:42:26.29 Intake @14.2psia, vacuum from 8.7 to 11.7psi @15:42:26.31 SS.reconfigure :inhaling 1.98ml/s @15:42:26.36 SS.seek 25ml @15:42:34.05 Stroke completed -- waiting for pressure to equalize ... @15:42:34.06 SS.reconfigure :exhale @15:42:34.11 SS.hold @15:42:42.58 Intake.select :close @15:42:42.72 SSV.dial :exhaust @15:42:43.47 Exhaust.select :open @15:42:43.58 SS.seek 0ml @15:42:52.41 Exhaust.select :close @15:42:52.52 SSV.dial CBV @15:42:52.54 CBV.dial SSV @15:42:53.30 Allowing 130s for 25.0ml of remaining 975.0ml @15:42:53.32 Intake.select :open @15:42:55.47 Intake @16.1psia, vacuum from 10.0 to 13.0psi @15:42:55.49 SS.reconfigure :inhaling 1.98ml/s @15:42:55.53 SS.seek 25ml @15:43:04.26 Vacuum too high -- waiting up to 40 seconds @15:43:04.27 SS.reconfigure :exhale @15:43:04.32 SS.hold @15:43:06.63 Intake @14.2psia, vacuum from 8.7 to 11.7psi @15:43:06.65 SS.reconfigure :inhaling 1.79ml/s @15:43:06.69 SS.seek 25ml @15:43:15.71 Stroke completed -- waiting for pressure to equalize ... @15:43:15.73 SS.reconfigure :exhale @15:43:15.77 SS.hold @15:43:24.35 Intake.select :close @15:43:24.50 SSV.dial :exhaust @15:43:25.26 Exhaust.select :open @15:43:25.37 SS.seek 0ml @15:43:34.13 Exhaust.select :close @15:43:34.24 SSV.dial CBV @15:43:34.27 CBV.dial SSV @15:43:35.01 Allowing 130s for 25.0ml of remaining 950.0ml @15:43:35.02 Intake.select :open @15:43:37.17 Intake @16.3psia, vacuum from 10.0 to 13.0psi @15:43:37.19 SS.reconfigure :inhaling 1.79ml/s @15:43:37.23 SS.seek 25ml @15:43:45.82 Vacuum too high -- waiting up to 40 seconds @15:43:45.84 SS.reconfigure :exhale @15:43:45.88 SS.hold @15:43:47.34 Intake @13.8psia, vacuum from 8.3 to 11.3psi @15:43:47.36 SS.reconfigure :inhaling 1.61ml/s @15:43:47.40 SS.seek 25ml @15:43:57.69 Stroke completed -- waiting for pressure to equalize ... @15:43:57.70 SS.reconfigure :exhale @15:43:57.74 SS.hold @15:44:05.72 Intake.select :close @15:44:05.86 SSV.dial :exhaust @15:44:06.63 Exhaust.select :open @15:44:06.74 SS.seek 0ml @15:44:15.60 Exhaust.select :close @15:44:15.70 SSV.dial CBV @15:44:15.72 CBV.dial SSV @15:44:16.49 Allowing 130s for 25.0ml of remaining 925.0ml @15:44:16.50 Intake.select :open @15:44:18.66 Intake @16.3psia, vacuum from 10.0 to 13.0psi @15:44:18.68 SS.reconfigure :inhaling 1.61ml/s @15:44:18.72 SS.seek 25ml @15:44:28.32 Vacuum too high -- waiting up to 40 seconds @15:44:28.33 SS.reconfigure :exhale @15:44:28.38 SS.hold @15:44:29.15 Intake @13.8psia, vacuum from 8.3 to 11.3psi @15:44:29.18 SS.reconfigure :inhaling 1.45ml/s @15:44:29.23 SS.seek 25ml @15:44:39.88 Stroke completed -- waiting for pressure to equalize ... @15:44:39.89 SS.reconfigure :exhale @15:44:39.93 SS.hold @15:44:48.39 Intake.select :close @15:44:48.54 SSV.dial :exhaust @15:44:49.28 Exhaust.select :open @15:44:49.39 SS.seek 0ml @15:44:58.47 Exhaust.select :close @15:44:58.58 SSV.dial CBV @15:44:58.63 CBV.dial SSV @15:44:59.33 Allowing 130s for 25.0ml of remaining 900.0ml @15:44:59.34 Intake.select :open @15:45:01.50 Intake @16.1psia, vacuum from 10.0 to 13.0psi @15:45:01.52 SS.reconfigure :inhaling 1.45ml/s @15:45:01.56 SS.seek 25ml @15:45:13.82 Vacuum too high -- waiting up to 40 seconds @15:45:13.83 SS.reconfigure :exhale @15:45:13.88 SS.hold @15:45:15.99 Intake @14.1psia, vacuum from 8.6 to 11.6psi @15:45:16.01 SS.reconfigure :inhaling 1.30ml/s @15:45:16.05 SS.seek 25ml @15:45:21.58 recoverPRVreagent08 @15:45:21.59 PTV.dial :mixing @15:45:22.33 PBV.dial :waste1 @15:45:22.71 delay 30 seconds @15:45:24.87 Stroke completed -- waiting for pressure to equalize ... @15:45:24.89 SS.reconfigure :exhale @15:45:24.93 SS.hold @15:45:31.44 Intake.select :close @15:45:31.58 SSV.dial :exhaust @15:45:32.33 Exhaust.select :open @15:45:32.44 SS.seek 0ml @15:45:41.28 Exhaust.select :close @15:45:41.39 SSV.dial CBV @15:45:41.41 CBV.dial SSV @15:45:42.17 Allowing 130s for 25.0ml of remaining 875.0ml @15:45:42.19 Intake.select :open @15:45:44.34 Intake @16.2psia, vacuum from 10.0 to 13.0psi @15:45:44.36 SS.reconfigure :inhaling 1.30ml/s @15:45:44.40 SS.seek 25ml @15:45:52.73 PBV.dial between PSV and waste1 @15:45:53.10 PSV.dial :air @15:45:53.57 PS.reconfigure :fast @15:45:53.63 PS.seek 2.5ml @15:45:58.07 Vacuum too high -- waiting up to 40 seconds @15:45:58.08 SS.reconfigure :exhale @15:45:58.63 SS.hold @15:46:00.28 Intake @14.3psia, vacuum from 8.8 to 11.8psi @15:46:00.30 SS.reconfigure :inhaling 1.17ml/s @15:46:00.35 SS.seek 25ml @15:46:10.42 Stroke completed -- waiting for pressure to equalize ... @15:46:10.43 SS.reconfigure :exhale @15:46:10.48 SS.hold @15:46:16.03 Intake.select :close @15:46:16.17 SSV.dial :exhaust @15:46:16.92 Exhaust.select :open @15:46:17.04 SS.seek 0ml @15:46:17.36 PSV.dial :mixloop @15:46:17.83 delay 2 seconds @15:46:19.85 PSV.dial :air @15:46:20.32 delay 2 seconds @15:46:22.34 PSV.dial :mixloop @15:46:22.80 delay 2 seconds @15:46:24.82 PSV.dial :air @15:46:25.29 PS.seek 0ml @15:46:26.06 Exhaust.select :close @15:46:26.17 SSV.dial CBV @15:46:26.19 CBV.dial SSV @15:46:26.96 Allowing 130s for 25.0ml of remaining 850.0ml @15:46:26.97 Intake.select :open @15:46:29.12 Intake @16.1psia, vacuum from 10.0 to 13.0psi @15:46:29.14 SS.reconfigure :inhaling 1.17ml/s @15:46:29.18 SS.seek 25ml @15:46:48.58 washCycleDA #1 of 2 @15:46:48.59 PSR.select :air @15:46:48.61 PSV.dial PSR @15:46:49.96 Pulling 0.5ml of PSR.wash @15:46:49.97 PSR.select :wash @15:46:50.09 PS.reconfigure :default @15:46:50.13 PS.seek 0.5ml @15:46:51.23 Vacuum too high -- waiting up to 40 seconds @15:46:51.24 SS.reconfigure :exhale @15:46:51.28 SS.hold @15:46:54.30 Intake @14.5psia, vacuum from 9.0 to 12.0psi @15:46:54.32 SS.reconfigure :inhaling 1.06ml/s @15:46:54.37 SS.seek 25ml @15:46:56.06 delay 5 seconds @15:46:56.95 Stroke completed -- waiting for pressure to equalize ... @15:46:56.96 SS.reconfigure :exhale @15:46:57.01 SS.hold @15:47:01.07 PSR.select :air @15:47:01.18 PS.seek 2ml @15:47:01.80 Intake.select :close @15:47:01.94 SSV.dial :exhaust @15:47:02.69 Exhaust.select :open @15:47:02.80 SS.seek 0ml @15:47:11.90 Exhaust.select :close @15:47:12.01 SSV.dial CBV @15:47:12.03 CBV.dial SSV @15:47:12.78 Allowing 130s for 25.0ml of remaining 825.0ml @15:47:12.79 Intake.select :open @15:47:14.95 Intake @16.2psia, vacuum from 10.0 to 13.0psi @15:47:14.97 SS.reconfigure :inhaling 1.06ml/s @15:47:15.01 SS.seek 25ml @15:47:17.37 PTV.dial :puck @15:47:18.10 PSV.dial PTV @15:47:18.83 PBV.dial :waste1 @15:47:19.27 PS.seek 1.5ml @15:47:25.21 delay 3 seconds @15:47:28.22 PBV.dial between PSV and waste1 @15:47:28.60 PS.seek 0ml @15:47:40.92 Stroke completed -- waiting for pressure to equalize ... @15:47:40.93 SS.reconfigure :exhale @15:47:40.97 SS.hold @15:47:44.96 delay 2:00 @15:47:48.77 Intake.select :close @15:47:48.91 SSV.dial :exhaust @15:47:49.65 Exhaust.select :open @15:47:49.77 SS.seek 0ml @15:47:58.64 Exhaust.select :close @15:47:58.75 SSV.dial CBV @15:47:58.78 CBV.dial SSV @15:47:59.52 Allowing 130s for 25.0ml of remaining 800.0ml @15:47:59.53 Intake.select :open @15:48:01.68 Intake @16.2psia, vacuum from 10.0 to 13.0psi @15:48:01.70 SS.reconfigure :inhaling 1.06ml/s @15:48:01.74 SS.seek 25ml @15:48:25.04 Vacuum too high -- waiting up to 40 seconds @15:48:25.05 SS.reconfigure :exhale @15:48:25.09 SS.hold @15:48:27.14 Intake @14.2psia, vacuum from 8.7 to 11.7psi @15:48:27.16 SS.reconfigure :inhaling 0.95ml/s @15:48:27.20 SS.seek 25ml @15:48:31.25 Stroke completed -- waiting for pressure to equalize ... @15:48:31.26 SS.reconfigure :exhale @15:48:31.30 SS.hold @15:48:37.06 Intake.select :close @15:48:37.21 SSV.dial :exhaust @15:48:37.95 Exhaust.select :open @15:48:38.06 SS.seek 0ml @15:48:46.90 Exhaust.select :close @15:48:47.01 SSV.dial CBV @15:48:47.03 CBV.dial SSV @15:48:47.80 Allowing 130s for 25.0ml of remaining 775.0ml @15:48:47.81 Intake.select :open @15:48:49.96 Intake @16.2psia, vacuum from 10.0 to 13.0psi @15:48:49.98 SS.reconfigure :inhaling 0.95ml/s @15:48:50.02 SS.seek 25ml @15:49:18.95 Stroke completed -- waiting for pressure to equalize ... @15:49:18.96 SS.reconfigure :exhale @15:49:19.00 SS.hold @15:49:27.45 Intake.select :close @15:49:27.60 SSV.dial :exhaust @15:49:28.34 Exhaust.select :open @15:49:28.45 SS.seek 0ml @15:49:37.60 Exhaust.select :close @15:49:37.71 SSV.dial CBV @15:49:37.73 CBV.dial SSV @15:49:38.49 Allowing 130s for 25.0ml of remaining 750.0ml @15:49:38.50 Intake.select :open @15:49:40.66 Intake @16.2psia, vacuum from 10.0 to 13.0psi @15:49:40.67 SS.reconfigure :inhaling 0.95ml/s @15:49:40.72 SS.seek 25ml @15:49:44.97 recoverPSRreagent08 @15:49:44.98 PTV.dial :puck @15:49:45.00 PBV.dial :waste1 @15:49:45.36 delay 30 seconds @15:50:08.31 Vacuum too high -- waiting up to 40 seconds @15:50:08.33 SS.reconfigure :exhale @15:50:08.37 SS.hold @15:50:12.27 Intake @14.8psia, vacuum from 9.3 to 12.3psi @15:50:12.29 SS.reconfigure :inhaling 0.87ml/s @15:50:12.34 SS.seek 25ml @15:50:13.28 Stroke completed -- waiting for pressure to equalize ... @15:50:13.29 SS.reconfigure :exhale @15:50:13.34 SS.hold @15:50:15.38 PBV.dial between PSV and waste1 @15:50:15.83 PSR.select :air @15:50:15.85 PSV.dial PSR @15:50:16.60 PS.reconfigure :fast @15:50:16.64 PS.seek 2.5ml @15:50:18.22 Intake.select :close @15:50:18.37 SSV.dial :exhaust @15:50:19.12 Exhaust.select :open @15:50:19.23 SS.seek 0ml @15:50:28.06 Exhaust.select :close @15:50:28.16 SSV.dial CBV @15:50:28.19 CBV.dial SSV @15:50:28.93 Allowing 130s for 25.0ml of remaining 725.0ml @15:50:28.95 Intake.select :open @15:50:31.10 Intake @16.2psia, vacuum from 10.0 to 13.0psi @15:50:31.12 SS.reconfigure :inhaling 0.87ml/s @15:50:31.16 SS.seek 25ml @15:50:40.33 PSV.dial PTV @15:50:41.08 delay 5 seconds @15:50:46.10 PSV.dial PSR @15:50:46.84 delay 5 seconds @15:50:51.86 PSV.dial PTV @15:50:52.74 delay 5 seconds @15:50:57.76 PSV.dial :air @15:50:58.52 PS.seek 0ml @15:51:03.15 Stroke completed -- waiting for pressure to equalize ... @15:51:03.17 SS.reconfigure :exhale @15:51:03.21 SS.hold @15:51:11.67 Intake.select :close @15:51:11.81 SSV.dial :exhaust @15:51:12.56 Exhaust.select :open @15:51:12.67 SS.seek 0ml @15:51:21.75 washCycleDA #2 of 2 @15:51:21.77 Exhaust.select :close @15:51:21.78 PSR.select :air @15:51:21.89 PSV.dial PSR @15:51:21.94 SSV.dial CBV @15:51:21.95 CBV.dial SSV @15:51:22.71 Allowing 130s for 25.0ml of remaining 700.0ml @15:51:22.73 Intake.select :open @15:51:23.24 Pulling 0.5ml of PSR.wash @15:51:23.25 PSR.select :wash @15:51:23.36 PS.reconfigure :default @15:51:23.41 PS.seek 0.5ml @15:51:24.88 Intake @16.1psia, vacuum from 10.0 to 13.0psi @15:51:24.90 SS.reconfigure :inhaling 0.87ml/s @15:51:24.94 SS.seek 25ml @15:51:29.39 delay 5 seconds @15:51:34.42 PSR.select :air @15:51:34.53 PS.seek 2ml @15:51:50.70 PTV.dial :puck @15:51:50.71 PSV.dial PTV @15:51:51.56 PBV.dial :waste1 @15:51:51.99 PS.seek 1.5ml @15:51:54.50 Vacuum too high -- waiting up to 40 seconds @15:51:54.52 SS.reconfigure :exhale @15:51:54.56 SS.hold @15:51:57.07 Intake @14.0psia, vacuum from 8.5 to 11.5psi @15:51:57.59 SS.reconfigure :inhaling 0.78ml/s @15:51:57.63 SS.seek 25ml @15:51:57.89 delay 3 seconds @15:52:00.23 Stroke completed -- waiting for pressure to equalize ... @15:52:00.24 SS.reconfigure :exhale @15:52:00.28 SS.hold @15:52:00.90 PBV.dial between PSV and waste1 @15:52:01.27 PS.seek 0ml @15:52:05.84 Intake.select :close @15:52:05.98 SSV.dial :exhaust @15:52:06.73 Exhaust.select :open @15:52:06.84 SS.seek 0ml @15:52:15.93 Exhaust.select :close @15:52:16.04 SSV.dial CBV @15:52:16.06 CBV.dial SSV @15:52:16.82 Allowing 130s for 25.0ml of remaining 675.0ml @15:52:16.84 Intake.select :open @15:52:17.58 delay 2:00 @15:52:18.99 Intake @16.1psia, vacuum from 10.0 to 13.0psi @15:52:19.01 SS.reconfigure :inhaling 0.78ml/s @15:52:19.05 SS.seek 25ml @15:52:53.16 Stroke completed -- waiting for pressure to equalize ... @15:52:53.17 SS.reconfigure :exhale @15:52:53.22 SS.hold @15:53:02.89 Intake.select :close @15:53:03.03 SSV.dial :exhaust @15:53:03.78 Exhaust.select :open @15:53:03.89 SS.seek 0ml @15:53:12.94 Exhaust.select :close @15:53:13.05 SSV.dial CBV @15:53:13.07 CBV.dial SSV @15:53:13.84 Allowing 130s for 25.0ml of remaining 650.0ml @15:53:13.85 Intake.select :open @15:53:16.01 Intake @16.0psia, vacuum from 10.0 to 13.0psi @15:53:16.03 SS.reconfigure :inhaling 0.78ml/s @15:53:16.07 SS.seek 25ml @15:53:50.52 Stroke completed -- waiting for pressure to equalize ... @15:53:50.53 SS.reconfigure :exhale @15:53:50.57 SS.hold @15:54:00.38 Intake.select :close @15:54:00.52 SSV.dial :exhaust @15:54:01.28 Exhaust.select :open @15:54:01.39 SS.seek 0ml @15:54:10.33 Exhaust.select :close @15:54:10.44 SSV.dial CBV @15:54:10.46 CBV.dial SSV @15:54:11.23 Allowing 130s for 25.0ml of remaining 625.0ml @15:54:11.24 Intake.select :open @15:54:13.39 Intake @16.0psia, vacuum from 10.0 to 13.0psi @15:54:13.41 SS.reconfigure :inhaling 0.78ml/s @15:54:13.45 SS.seek 25ml @15:54:17.60 recoverPSRreagent08 @15:54:17.61 PTV.dial :puck @15:54:17.62 PBV.dial :waste1 @15:54:17.96 delay 30 seconds @15:54:42.56 Vacuum too high -- waiting up to 40 seconds @15:54:42.58 SS.reconfigure :exhale @15:54:42.62 SS.hold @15:54:45.23 Intake @14.0psia, vacuum from 8.5 to 11.5psi @15:54:45.25 SS.reconfigure :inhaling 0.70ml/s @15:54:45.29 SS.seek 25ml @15:54:47.98 PBV.dial between PSV and waste1 @15:54:48.34 PSR.select :air @15:54:48.36 PSV.dial PSR @15:54:49.09 PS.reconfigure :fast @15:54:49.14 PS.seek 2.5ml @15:54:52.04 Stroke completed -- waiting for pressure to equalize ... @15:54:52.05 SS.reconfigure :exhale @15:54:52.09 SS.hold @15:55:00.56 Intake.select :close @15:55:00.70 SSV.dial :exhaust @15:55:01.44 Exhaust.select :open @15:55:01.55 SS.seek 0ml @15:55:10.71 Exhaust.select :close @15:55:10.86 SSV.dial CBV @15:55:10.88 CBV.dial SSV @15:55:11.63 Allowing 130s for 25.0ml of remaining 600.0ml @15:55:11.64 Intake.select :open @15:55:12.79 PSV.dial PTV @15:55:13.54 delay 5 seconds @15:55:13.80 Intake @16.0psia, vacuum from 10.0 to 13.0psi @15:55:13.82 SS.reconfigure :inhaling 0.70ml/s @15:55:13.86 SS.seek 25ml @15:55:18.56 PSV.dial PSR @15:55:19.31 delay 5 seconds @15:55:24.33 PSV.dial PTV @15:55:25.07 delay 5 seconds @15:55:30.09 PSV.dial :air @15:55:30.84 PS.seek 0ml @15:55:51.39 Stroke completed -- waiting for pressure to equalize ... @15:55:51.41 SS.reconfigure :exhale @15:55:51.45 SS.hold @15:55:53.98 getPMLReagent08 @15:55:53.99 PRV.dial :air @15:55:54.43 delay 5 seconds @15:55:59.45 PTV.dial :PRVmixing @15:56:00.76 delay 5 seconds @15:56:04.05 Intake.select :close @15:56:04.19 SSV.dial :exhaust @15:56:04.93 Exhaust.select :open @15:56:05.04 SS.seek 0ml @15:56:05.77 PRV.dial :ab2 @15:56:06.23 PTV.dial :PRVmixing @15:56:06.24 PSV.dial :mixloop @15:56:06.70 PS.reconfigure :default @15:56:06.75 PS.seek 0.3ml @15:56:10.73 delay 2 seconds @15:56:12.74 PRV.dial :air @15:56:13.27 PS.seek 0.55ml @15:56:13.91 Exhaust.select :close @15:56:14.02 SSV.dial CBV @15:56:14.04 CBV.dial SSV @15:56:14.80 Allowing 130s for 25.0ml of remaining 575.0ml @15:56:14.82 Intake.select :open @15:56:16.57 addPMLReagent08 @15:56:16.58 PSV.dial :air @15:56:16.97 Intake @16.0psia, vacuum from 10.0 to 13.0psi @15:56:16.99 SS.reconfigure :inhaling 0.70ml/s @15:56:17.04 SS.seek 25ml @15:56:17.10 PS.seek 2ml @15:56:32.79 PSV.dial :PML @15:56:33.26 PTV.dial :mixing @15:56:34.01 PBV.dial :waste1 @15:56:34.44 PS.seek 1.85ml @15:56:36.84 PBV.dial between PSV and waste1 @15:56:37.22 PS.seek 0ml @15:56:49.03 Vacuum too high -- waiting up to 40 seconds @15:56:49.04 SS.reconfigure :exhale @15:56:49.08 SS.hold @15:56:52.64 Intake @14.2psia, vacuum from 8.7 to 11.7psi @15:56:52.66 SS.reconfigure :inhaling 0.63ml/s @15:56:52.70 SS.seek 25ml @15:56:56.94 delay 2 seconds @15:56:58.96 delay 8:00 @15:56:59.75 Stroke completed -- waiting for pressure to equalize ... @15:56:59.76 SS.reconfigure :exhale @15:56:59.81 SS.hold @15:57:08.30 Intake.select :close @15:57:08.44 SSV.dial :exhaust @15:57:09.19 Exhaust.select :open @15:57:09.30 SS.seek 0ml @15:57:18.38 Exhaust.select :close @15:57:18.48 SSV.dial CBV @15:57:18.50 CBV.dial SSV @15:57:19.27 Allowing 130s for 25.0ml of remaining 550.0ml @15:57:19.28 Intake.select :open @15:57:21.44 Intake @16.3psia, vacuum from 10.0 to 13.0psi @15:57:21.46 SS.reconfigure :inhaling 0.63ml/s @15:57:21.50 SS.seek 25ml @15:57:57.56 Vacuum too high -- waiting up to 40 seconds @15:57:57.57 SS.reconfigure :exhale @15:57:57.61 SS.hold @15:58:00.62 Intake @14.5psia, vacuum from 9.0 to 12.0psi @15:58:00.64 SS.reconfigure :inhaling 0.57ml/s @15:58:00.68 SS.seek 25ml @15:58:07.55 Stroke completed -- waiting for pressure to equalize ... @15:58:07.56 SS.reconfigure :exhale @15:58:07.60 SS.hold @15:58:16.06 Intake.select :close @15:58:16.20 SSV.dial :exhaust @15:58:16.95 Exhaust.select :open @15:58:17.06 SS.seek 0ml @15:58:26.12 Exhaust.select :close @15:58:26.23 SSV.dial CBV @15:58:26.25 CBV.dial SSV @15:58:27.02 Allowing 130s for 25.0ml of remaining 525.0ml @15:58:27.03 Intake.select :open @15:58:29.19 Intake @16.3psia, vacuum from 10.0 to 13.0psi @15:58:29.20 SS.reconfigure :inhaling 0.57ml/s @15:58:29.25 SS.seek 25ml @15:59:15.25 Stroke completed -- waiting for pressure to equalize ... @15:59:15.26 SS.reconfigure :exhale @15:59:15.30 SS.hold @15:59:27.92 Intake.select :close @15:59:28.07 SSV.dial :exhaust @15:59:28.81 Exhaust.select :open @15:59:28.92 SS.seek 0ml @15:59:37.83 Exhaust.select :close @15:59:37.94 SSV.dial CBV @15:59:37.96 CBV.dial SSV @15:59:38.72 Allowing 130s for 25.0ml of remaining 500.0ml @15:59:38.73 Intake.select :open @15:59:40.89 Intake @16.2psia, vacuum from 10.0 to 13.0psi @15:59:40.91 SS.reconfigure :inhaling 0.57ml/s @15:59:40.96 SS.seek 25ml @16:00:19.62 Vacuum too high -- waiting up to 40 seconds @16:00:19.63 SS.reconfigure :exhale @16:00:19.67 SS.hold @16:00:24.56 Intake @15.1psia, vacuum from 9.6 to 12.6psi @16:00:24.58 SS.reconfigure :inhaling 0.51ml/s @16:00:24.63 SS.seek 25ml @16:00:33.62 Stroke completed -- waiting for pressure to equalize ... @16:00:33.63 SS.reconfigure :exhale @16:00:33.68 SS.hold @16:00:43.40 Intake.select :close @16:00:43.54 SSV.dial :exhaust @16:00:44.30 Exhaust.select :open @16:00:44.41 SS.seek 0ml @16:00:53.28 Exhaust.select :close @16:00:53.38 SSV.dial CBV @16:00:53.42 CBV.dial SSV @16:00:54.16 Allowing 130s for 25.0ml of remaining 475.0ml @16:00:54.17 Intake.select :open @16:00:56.32 Intake @16.3psia, vacuum from 10.0 to 13.0psi @16:00:56.34 SS.reconfigure :inhaling 0.51ml/s @16:00:56.38 SS.seek 25ml @16:01:47.84 Stroke completed -- waiting for pressure to equalize ... @16:01:47.85 SS.reconfigure :exhale @16:01:47.89 SS.hold @16:02:02.19 Intake.select :close @16:02:02.34 SSV.dial :exhaust @16:02:03.09 Exhaust.select :open @16:02:03.20 SS.seek 0ml @16:02:12.09 Exhaust.select :close @16:02:12.20 SSV.dial CBV @16:02:12.24 CBV.dial SSV @16:02:12.97 Allowing 130s for 25.0ml of remaining 450.0ml @16:02:12.98 Intake.select :open @16:02:15.13 Intake @16.2psia, vacuum from 10.0 to 13.0psi @16:02:15.15 SS.reconfigure :inhaling 0.51ml/s @16:02:15.19 SS.seek 25ml @16:02:59.53 Vacuum too high -- waiting up to 40 seconds @16:02:59.55 SS.reconfigure :exhale @16:02:59.59 SS.hold @16:03:04.47 Intake @15.2psia, vacuum from 9.7 to 12.7psi @16:03:04.49 SS.reconfigure :inhaling 0.47ml/s @16:03:04.53 SS.seek 25ml @16:03:13.27 Stroke completed -- waiting for pressure to equalize ... @16:03:13.29 SS.reconfigure :exhale @16:03:13.33 SS.hold @16:03:23.01 Intake.select :close @16:03:23.15 SSV.dial :exhaust @16:03:23.90 Exhaust.select :open @16:03:24.01 SS.seek 0ml @16:03:32.85 Exhaust.select :close @16:03:32.96 SSV.dial CBV @16:03:32.99 CBV.dial SSV @16:03:33.73 Allowing 130s for 25.0ml of remaining 425.0ml @16:03:33.74 Intake.select :open @16:03:35.90 Intake @16.3psia, vacuum from 10.0 to 13.0psi @16:03:35.92 SS.reconfigure :inhaling 0.47ml/s @16:03:35.96 SS.seek 25ml @16:04:30.67 Vacuum too high -- waiting up to 40 seconds @16:04:30.68 SS.reconfigure :exhale @16:04:30.73 SS.hold @16:04:34.90 Intake @14.9psia, vacuum from 9.4 to 12.4psi @16:04:34.92 SS.reconfigure :inhaling 0.43ml/s @16:04:34.96 SS.seek 25ml @16:04:37.80 Stroke completed -- waiting for pressure to equalize ... @16:04:37.82 SS.reconfigure :exhale @16:04:37.86 SS.hold @16:04:47.66 Intake.select :close @16:04:47.80 SSV.dial :exhaust @16:04:48.54 Exhaust.select :open @16:04:48.65 SS.seek 0ml @16:04:57.88 Exhaust.select :close @16:04:57.99 SSV.dial CBV @16:04:58.01 CBV.dial SSV @16:04:58.78 Allowing 130s for 25.0ml of remaining 400.0ml @16:04:58.79 Intake.select :open @16:04:58.98 recoverPRVreagent08 @16:04:58.99 PTV.dial :mixing @16:04:59.00 PBV.dial :waste1 @16:04:59.37 delay 30 seconds @16:05:00.94 Intake @16.2psia, vacuum from 10.0 to 13.0psi @16:05:00.96 SS.reconfigure :inhaling 0.43ml/s @16:05:01.00 SS.seek 25ml @16:05:29.39 PBV.dial between PSV and waste1 @16:05:29.76 PSV.dial :air @16:05:30.24 PS.reconfigure :fast @16:05:30.28 PS.seek 2.5ml @16:05:53.93 PSV.dial :mixloop @16:05:54.40 delay 2 seconds @16:05:56.42 PSV.dial :air @16:05:57.40 delay 2 seconds @16:05:59.42 PSV.dial :mixloop @16:05:59.89 delay 2 seconds @16:06:01.90 PSV.dial :air @16:06:02.38 PS.seek 0ml @16:06:04.35 Stroke completed -- waiting for pressure to equalize ... @16:06:04.36 SS.reconfigure :exhale @16:06:04.40 SS.hold @16:06:18.71 Intake.select :close @16:06:18.85 SSV.dial :exhaust @16:06:19.60 Exhaust.select :open @16:06:19.71 SS.seek 0ml @16:06:25.56 washCycleDA #1 of 3 @16:06:25.57 PSR.select :air @16:06:25.59 PSV.dial PSR @16:06:26.94 Pulling 0.5ml of PSR.wash @16:06:26.95 PSR.select :wash @16:06:27.06 PS.reconfigure :default @16:06:27.11 PS.seek 0.5ml @16:06:28.71 Exhaust.select :close @16:06:28.82 SSV.dial CBV @16:06:28.87 CBV.dial SSV @16:06:29.57 Allowing 130s for 25.0ml of remaining 375.0ml @16:06:29.58 Intake.select :open @16:06:31.74 Intake @16.2psia, vacuum from 10.0 to 13.0psi @16:06:31.76 SS.reconfigure :inhaling 0.43ml/s @16:06:31.80 SS.seek 25ml @16:06:33.03 delay 5 seconds @16:06:38.05 PSR.select :air @16:06:38.16 PS.seek 2ml @16:06:54.27 PTV.dial :puck @16:06:55.01 PSV.dial PTV @16:06:55.74 PBV.dial :waste1 @16:06:56.18 PS.seek 1.5ml @16:07:02.18 delay 3 seconds @16:07:05.20 PBV.dial between PSV and waste1 @16:07:05.58 PS.seek 0ml @16:07:21.78 delay 2:00 @16:07:35.19 Stroke completed -- waiting for pressure to equalize ... @16:07:35.20 SS.reconfigure :exhale @16:07:35.25 SS.hold @16:07:51.43 Intake.select :close @16:07:51.57 SSV.dial :exhaust @16:07:52.32 Exhaust.select :open @16:07:52.43 SS.seek 0ml @16:08:01.35 Exhaust.select :close @16:08:01.46 SSV.dial CBV @16:08:01.47 CBV.dial SSV @16:08:02.22 Allowing 130s for 25.0ml of remaining 350.0ml @16:08:02.24 Intake.select :open @16:08:04.39 Intake @16.2psia, vacuum from 10.0 to 13.0psi @16:08:04.41 SS.reconfigure :inhaling 0.43ml/s @16:08:04.45 SS.seek 25ml @16:09:00.23 Vacuum too high -- waiting up to 40 seconds @16:09:00.24 SS.reconfigure :exhale @16:09:00.28 SS.hold @16:09:05.24 Intake @14.8psia, vacuum from 9.3 to 12.3psi @16:09:05.26 SS.reconfigure :inhaling 0.38ml/s @16:09:05.31 SS.seek 25ml @16:09:14.74 Stroke completed -- waiting for pressure to equalize ... @16:09:14.75 SS.reconfigure :exhale @16:09:14.79 SS.hold @16:09:21.80 recoverPSRreagent08 @16:09:21.81 PTV.dial :puck @16:09:21.82 PBV.dial :waste1 @16:09:22.18 delay 30 seconds @16:09:27.36 Intake.select :close @16:09:27.50 SSV.dial :exhaust @16:09:28.25 Exhaust.select :open @16:09:28.36 SS.seek 0ml @16:09:37.31 Exhaust.select :close @16:09:37.42 SSV.dial CBV @16:09:37.44 CBV.dial SSV @16:09:38.21 Allowing 130s for 25.0ml of remaining 325.0ml @16:09:38.22 Intake.select :open @16:09:40.38 Intake @16.3psia, vacuum from 10.0 to 13.0psi @16:09:40.40 SS.reconfigure :inhaling 0.38ml/s @16:09:40.45 SS.seek 25ml @16:09:52.20 PBV.dial between PSV and waste1 @16:09:52.59 PSR.select :air @16:09:52.61 PSV.dial PSR @16:09:53.36 PS.reconfigure :fast @16:09:53.40 PS.seek 2.5ml @16:10:17.11 PSV.dial PTV @16:10:17.99 delay 5 seconds @16:10:23.01 PSV.dial PSR @16:10:23.74 delay 5 seconds @16:10:28.76 PSV.dial PTV @16:10:29.51 delay 5 seconds @16:10:34.53 PSV.dial :air @16:10:35.28 PS.seek 0ml @16:10:54.23 Stroke completed -- waiting for pressure to equalize ... @16:10:54.25 SS.reconfigure :exhale @16:10:54.29 SS.hold @16:10:58.43 washCycleDA #2 of 3 @16:10:58.44 PSR.select :air @16:10:58.45 PSV.dial PSR @16:10:59.83 Pulling 0.5ml of PSR.wash @16:10:59.84 PSR.select :wash @16:10:59.96 PS.reconfigure :default @16:11:00.00 PS.seek 0.5ml @16:11:06.05 delay 5 seconds @16:11:10.50 Intake.select :close @16:11:10.65 SSV.dial :exhaust @16:11:11.07 PSR.select :air @16:11:11.18 PS.seek 2ml @16:11:11.38 Exhaust.select :open @16:11:11.49 SS.seek 0ml @16:11:20.39 Exhaust.select :close @16:11:20.50 SSV.dial CBV @16:11:20.53 CBV.dial SSV @16:11:21.27 Allowing 130s for 25.0ml of remaining 300.0ml @16:11:21.28 Intake.select :open @16:11:23.44 Intake @16.4psia, vacuum from 10.0 to 13.0psi @16:11:23.46 SS.reconfigure :inhaling 0.38ml/s @16:11:23.51 SS.seek 25ml @16:11:27.52 PTV.dial :puck @16:11:27.53 PSV.dial PTV @16:11:28.38 PBV.dial :waste1 @16:11:28.81 PS.seek 1.5ml @16:11:34.77 delay 3 seconds @16:11:37.79 PBV.dial between PSV and waste1 @16:11:38.15 PS.seek 0ml @16:11:54.99 delay 2:00 @16:12:34.82 Vacuum too high -- waiting up to 40 seconds @16:12:34.84 SS.reconfigure :exhale @16:12:34.88 SS.hold @16:12:39.63 Intake @14.8psia, vacuum from 9.3 to 12.3psi @16:12:39.65 SS.reconfigure :inhaling 0.34ml/s @16:12:39.69 SS.seek 25ml @16:12:42.64 Stroke completed -- waiting for pressure to equalize ... @16:12:42.65 SS.reconfigure :exhale @16:12:42.69 SS.hold @16:12:55.25 Intake.select :close @16:12:55.40 SSV.dial :exhaust @16:12:56.14 Exhaust.select :open @16:12:56.25 SS.seek 0ml @16:13:05.17 Exhaust.select :close @16:13:05.28 SSV.dial CBV @16:13:05.32 CBV.dial SSV @16:13:06.05 Allowing 130s for 25.0ml of remaining 275.0ml @16:13:06.06 Intake.select :open @16:13:08.21 Intake @16.2psia, vacuum from 10.0 to 13.0psi @16:13:08.23 SS.reconfigure :inhaling 0.34ml/s @16:13:08.27 SS.seek 25ml @16:13:55.01 recoverPSRreagent08 @16:13:55.02 PTV.dial :puck @16:13:55.03 PBV.dial :waste1 @16:13:55.40 delay 30 seconds @16:14:25.42 PBV.dial between PSV and waste1 @16:14:25.78 PSR.select :air @16:14:25.80 PSV.dial PSR @16:14:26.55 PS.reconfigure :fast @16:14:26.60 PS.seek 2.5ml @16:14:33.95 Stroke completed -- waiting for pressure to equalize ... @16:14:33.96 SS.reconfigure :exhale @16:14:34.00 SS.hold @16:14:50.20 Intake.select :close @16:14:50.26 PSV.dial PTV @16:14:50.36 SSV.dial :exhaust @16:14:51.03 delay 5 seconds @16:14:51.11 Exhaust.select :open @16:14:51.22 SS.seek 0ml @16:14:56.05 PSV.dial PSR @16:14:56.80 delay 5 seconds @16:15:00.40 Exhaust.select :close @16:15:00.51 SSV.dial CBV @16:15:00.54 CBV.dial SSV @16:15:01.30 Allowing 130s for 25.0ml of remaining 250.0ml @16:15:01.32 Intake.select :open @16:15:01.81 PSV.dial PTV @16:15:02.56 delay 5 seconds @16:15:03.47 Intake @16.3psia, vacuum from 10.0 to 13.0psi @16:15:03.49 SS.reconfigure :inhaling 0.34ml/s @16:15:03.54 SS.seek 25ml @16:15:07.58 PSV.dial :air @16:15:08.33 PS.seek 0ml @16:15:31.55 washCycleDA #3 of 3 @16:15:31.56 PSR.select :air @16:15:31.57 PSV.dial PSR @16:15:33.45 Pulling 0.5ml of PSR.wash @16:15:33.46 PSR.select :wash @16:15:33.58 PS.reconfigure :default @16:15:33.62 PS.seek 0.5ml @16:15:39.61 delay 5 seconds @16:15:44.63 PSR.select :air @16:15:44.74 PS.seek 2ml @16:16:00.89 PTV.dial :puck @16:16:00.90 PSV.dial PTV @16:16:01.62 PBV.dial :waste1 @16:16:02.06 PS.seek 1.5ml @16:16:07.95 delay 3 seconds @16:16:10.97 PBV.dial between PSV and waste1 @16:16:11.35 PS.seek 0ml @16:16:27.58 delay 2:00 @16:16:29.24 Stroke completed -- waiting for pressure to equalize ... @16:16:29.25 SS.reconfigure :exhale @16:16:29.30 SS.hold @16:16:49.60 Intake.select :close @16:16:49.74 SSV.dial :exhaust @16:16:50.49 Exhaust.select :open @16:16:50.61 SS.seek 0ml @16:16:59.54 Exhaust.select :close @16:16:59.65 SSV.dial CBV @16:16:59.67 CBV.dial SSV @16:17:00.43 Allowing 130s for 25.0ml of remaining 225.0ml @16:17:00.44 Intake.select :open @16:17:02.60 Intake @16.3psia, vacuum from 10.0 to 13.0psi @16:17:02.62 SS.reconfigure :inhaling 0.34ml/s @16:17:02.66 SS.seek 25ml @16:18:27.60 recoverPSRreagent08 @16:18:27.61 PTV.dial :puck @16:18:27.62 PBV.dial :waste1 @16:18:27.98 delay 30 seconds @16:18:30.35 Stroke completed -- waiting for pressure to equalize ... @16:18:30.36 SS.reconfigure :exhale @16:18:30.40 SS.hold @16:18:52.74 Intake.select :close @16:18:53.66 SSV.dial :exhaust @16:18:54.40 Exhaust.select :open @16:18:54.51 SS.seek 0ml @16:18:58.00 PBV.dial between PSV and waste1 @16:18:58.37 PSR.select :air @16:18:58.39 PSV.dial PSR @16:18:59.27 PS.reconfigure :fast @16:18:59.32 PS.seek 2.5ml @16:19:03.43 Exhaust.select :close @16:19:03.54 SSV.dial CBV @16:19:03.57 CBV.dial SSV @16:19:04.33 Allowing 130s for 25.0ml of remaining 200.0ml @16:19:04.34 Intake.select :open @16:19:06.49 Intake @16.6psia, vacuum from 10.0 to 13.0psi @16:19:06.51 SS.reconfigure :inhaling 0.34ml/s @16:19:06.55 SS.seek 25ml @16:19:22.98 PSV.dial PTV @16:19:23.71 delay 5 seconds @16:19:28.73 PSV.dial PSR @16:19:29.48 delay 5 seconds @16:19:34.50 PSV.dial PTV @16:19:35.24 delay 5 seconds @16:19:40.26 PSV.dial :air @16:19:41.54 PS.seek 0ml @16:20:04.45 Gate.power :camera,:ON @16:20:04.46 Mixing DA Substrates @16:20:04.47 PSR.select :air @16:20:04.48 PSV.dial PSR @16:20:06.02 Pulling 0.25ml of PSR.sub1 @16:20:06.05 PSR.select :sub1 @16:20:06.26 PS.reconfigure :default @16:20:06.38 PS.seek 0.25ml @16:20:09.90 delay 5 seconds @16:20:14.97 Pulling 0.25ml of PSR.sub2 @16:20:14.98 PSR.select :sub2 @16:20:15.10 PS.seek 0.5ml @16:20:18.54 delay 5 seconds @16:20:20.56 Vacuum too high -- waiting up to 40 seconds @16:20:20.57 SS.reconfigure :exhale @16:20:20.62 SS.hold @16:20:23.57 PSR.select :air @16:20:23.68 PS.seek 2ml @16:20:29.18 Intake @15.9psia, vacuum from 10.0 to 13.0psi @16:20:29.20 SS.reconfigure :inhaling 0.31ml/s @16:20:29.24 SS.seek 25ml @16:20:39.85 PTV.dial :puck @16:20:39.86 PSV.dial PTV @16:20:40.60 PBV.dial :waste1 @16:20:41.04 PS.seek 1.5ml @16:20:45.20 Stroke completed -- waiting for pressure to equalize ... @16:20:45.22 SS.reconfigure :exhale @16:20:45.26 SS.hold @16:20:47.04 delay 3 seconds @16:20:50.05 PBV.dial between PSV and waste1 @16:20:50.44 PS.seek 0ml @16:21:03.49 Intake.select :close @16:21:03.64 SSV.dial :exhaust @16:21:04.43 Exhaust.select :open @16:21:04.54 SS.seek 0ml @16:21:06.60 delay 10 seconds @16:21:13.38 Exhaust.select :close @16:21:13.48 SSV.dial CBV @16:21:13.50 CBV.dial SSV @16:21:14.27 Allowing 130s for 25.0ml of remaining 175.0ml @16:21:14.28 Intake.select :open @16:21:16.44 Intake @16.4psia, vacuum from 10.0 to 13.0psi @16:21:16.46 SS.reconfigure :inhaling 0.31ml/s @16:21:16.50 SS.seek 25ml @16:21:16.62 recoverPSRreagent08 @16:21:16.63 PTV.dial :puck @16:21:16.64 PBV.dial :waste1 @16:21:17.01 delay 30 seconds @16:21:47.03 PBV.dial between PSV and waste1 @16:21:47.40 PSR.select :air @16:21:47.42 PSV.dial PSR @16:21:48.18 PS.reconfigure :fast @16:21:48.23 PS.seek 2.5ml @16:22:12.32 PSV.dial PTV @16:22:13.09 delay 5 seconds @16:22:18.11 PSV.dial PSR @16:22:18.85 delay 5 seconds @16:22:23.87 PSV.dial PTV @16:22:24.63 delay 5 seconds @16:22:29.65 PSV.dial :air @16:22:30.42 PS.seek 0ml @16:22:53.73 Stroke completed -- waiting for pressure to equalize ... @16:22:53.75 SS.reconfigure :exhale @16:22:53.79 SS.hold @16:22:53.94 Additional air clear @16:22:53.95 PSR.select :air @16:22:53.97 PSV.dial PSR @16:22:55.40 Pulling 2ml of PSR.air @16:22:55.41 PSR.select :air @16:22:55.48 PS.reconfigure :default @16:22:56.09 PS.seek 2ml @16:23:16.70 Intake.select :close @16:23:16.85 SSV.dial :exhaust @16:23:17.38 delay 5 seconds @16:23:17.59 Exhaust.select :open @16:23:17.71 SS.seek 0ml @16:23:22.40 PTV.dial :puck @16:23:22.41 PSV.dial PTV @16:23:23.15 PBV.dial :waste1 @16:23:23.58 PS.seek 1.5ml @16:23:26.58 Exhaust.select :close @16:23:26.69 SSV.dial CBV @16:23:26.71 CBV.dial SSV @16:23:27.49 Allowing 130s for 25.0ml of remaining 150.0ml @16:23:27.50 Intake.select :open @16:23:29.60 delay 3 seconds @16:23:29.67 Intake @16.6psia, vacuum from 10.0 to 13.0psi @16:23:29.69 SS.reconfigure :inhaling 0.31ml/s @16:23:29.73 SS.seek 25ml @16:23:32.62 PBV.dial between PSV and waste1 @16:23:32.99 delay 1 second @16:23:34.01 PSV.dial :waste1 @16:23:35.07 PS.seek 0ml @16:23:51.27 Processing Puck Heater at 28C before imaging @16:23:51.28 PH.coast @16:23:51.40 Imaging da puck in Processing Clamp while storing it to tube 3 @16:23:51.56 SC.reconfigure :tight @16:23:51.61 SC.seek 3 @16:23:55.23 SC.configure :limp @16:23:55.42 Elbow.reconfigure :tight @16:23:55.47 Elbow.seek 3 @16:23:57.15 Forearm.reconfigure :tight @16:23:57.20 Forearm.seek 3 + 450 counts @16:23:58.24 SE.reconfigure :pick @16:23:58.29 SE.seek :beamBreak @16:23:59.56 SE.configure :default @16:23:59.71 Elbow.reconfigure :limp @16:23:59.76 Forearm.reconfigure :loose @16:23:59.80 Forearm.seek 3 @16:24:00.01 Elbow.hold @16:24:00.07 Elbow.reconfigure :tight @16:24:00.16 SE.seek 27.09 Pucks below Top Plate @16:24:00.71 Forearm.force -20 @16:24:00.75 Forearm.seek 3 + 317 counts @16:24:01.03 Forearm.reconfigure :tight @16:24:01.08 Forearm.seek 3 + 450 counts @16:24:01.28 SE.seek 28.09 Pucks below Top Plate @16:24:01.86 Forearm.reconfigure :loose @16:24:01.90 Forearm.seek :retract @16:24:02.81 Elbow.seek PC @16:24:05.31 Elbow.reconfigure :loose @16:24:05.40 Forearm.seek PC @16:24:06.36 Hand.close @16:24:07.03 PC.seek :open @16:24:11.82 Forearm.seek :retract @16:24:12.78 Elbow.reconfigure :tight @16:24:12.82 Elbow.seek 3 @16:24:15.42 Forearm.reconfigure :tight @16:24:15.46 Forearm.seek 3 + 450 counts @16:24:16.41 Hand.open @16:24:17.08 SE.seek 27.74 Pucks below Top Plate @16:24:17.33 SE.reconfigure :seal @16:24:17.38 SE.seek 26.78 Pucks below Top Plate @16:24:17.92 Lights, camera, ACTION! @16:24:18.49 PuckCamera: snap -bin=2,2 -auto /var/log/esp/da23mar2216h825ml.tif @16:24:37.43 Waiting >20 seconds for da to relinquish FlushPuck @16:24:51.88 Vacuum too high -- waiting up to 40 seconds @16:24:51.90 SS.reconfigure :exhale @16:24:51.95 SS.hold @16:24:59.34 Intake @15.8psia, vacuum from 10.0 to 13.0psi @16:24:59.36 SS.reconfigure :inhaling 0.28ml/s @16:24:59.40 SS.seek 25ml @16:25:15.50 Stroke completed -- waiting for pressure to equalize ... @16:25:15.51 SS.reconfigure :exhale @16:25:15.55 SS.hold @16:25:35.83 Intake.select :close @16:25:35.97 SSV.dial :exhaust @16:25:36.74 Exhaust.select :open @16:25:36.85 SS.seek 0ml @16:25:45.96 Exhaust.select :close @16:25:46.07 SSV.dial CBV @16:25:46.09 CBV.dial SSV @16:25:46.87 Allowing 130s for 25.0ml of remaining 125.0ml @16:25:46.89 Intake.select :open @16:25:49.04 Intake @16.5psia, vacuum from 10.0 to 13.0psi @16:25:49.06 SS.reconfigure :inhaling 0.28ml/s @16:25:49.10 SS.seek 25ml @16:25:57.80 SX HX9/16 (USB:24): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 95.945 seconds /var/log/esp/da23mar2216h825ml.tif: TIFF Upload Complete @16:25:57.82 PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da23mar2216h825ml15s.tif @16:26:13.47 SX HX9/16 (USB:24): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15 seconds /var/log/esp/midres/da23mar2216h825ml15s.tif: TIFF Upload Complete @16:26:13.49 PuckCamera: snap -bin=2,2 20.0 /var/log/esp/midres/da23mar2216h825ml20s.tif @16:26:34.11 SX HX9/16 (USB:24): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 20 seconds /var/log/esp/midres/da23mar2216h825ml20s.tif: TIFF Upload Complete @16:26:34.12 Gate.power :camera,:OFF @16:26:34.14 DAfans.email "95.945 sec auto-exposure",Subject: "da23mar2216h825ml images!" @16:26:34.40 Elbow.seek 3 @16:26:34.72 Forearm.seek 3 + 450 counts @16:26:34.80 SE.reconfigure :gently @16:26:34.85 SE.seek 27.49 Pucks below Top Plate @16:26:35.54 Elbow.reconfigure :limp @16:26:35.59 Forearm.reconfigure :loose @16:26:35.63 Forearm.seek 3 @16:26:35.86 Elbow.hold @16:26:35.94 Elbow.reconfigure :tight @16:26:35.99 SE.reconfigure :default @16:26:36.04 SE.seek 27.68 Pucks below Top Plate @16:26:36.52 Forearm.force -20 @16:26:36.56 Forearm.seek 3 + 309 counts @16:26:36.87 Forearm.reconfigure :tight @16:26:36.91 Forearm.seek 3 + 450 counts @16:26:37.19 SE.seek :down @16:26:38.77 SE.stop @16:26:38.94 Forearm.reconfigure :loose @16:26:38.99 Forearm.seek :retract @16:26:40.09 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @16:26:40.46 Elbow.seek FlushPuck::Garage @16:26:42.33 Elbow.reconfigure :loose @16:26:42.52 Forearm.seek :garage @16:26:43.56 Hand.close @16:26:44.47 Forearm.seek :retract @16:26:45.55 Elbow.reconfigure :tight @16:26:45.67 Elbow.seek PC @16:26:47.22 Elbow.reconfigure :loose @16:26:47.44 Forearm.reconfigure :tight @16:26:47.53 Forearm.seek PC @16:26:49.08 PC.seek :closed @16:26:53.84 Processing Clamp onSpring/offSpring for 16/26 samples, on/off torque 1.61 @16:26:54.00 Hand.open @16:26:54.79 Forearm.reconfigure :loose @16:26:54.87 Forearm.seek :retract @16:26:55.84 PSR.select :air @16:26:55.88 PSV.dial PSR @16:26:56.50 Pulling 0.5ml of PSR.flush @16:26:56.52 PSR.select :flush @16:26:56.70 PS.seek 0.5ml @16:27:02.68 delay 5 seconds @16:27:07.74 PSR.select :air @16:27:07.94 PS.seek 2.5ml @16:27:19.19 upsync @16:27:29.28 delay 1 second @16:27:30.30 PRV.dial :waste2,avoiding: :ab2 @16:27:32.14 PTV.dial :PRVmixing @16:27:33.45 PSV.dial :mixloop @16:27:34.49 PS.seek :empty @16:27:37.37 Stroke completed -- waiting for pressure to equalize ... @16:27:37.38 SS.reconfigure :exhale @16:27:37.42 SS.hold @16:27:56.90 SS.reconfigure :default @16:27:56.94 SS.hold @16:27:57.05 Intake.select :close @16:27:57.20 ShallowSampler::Clogged in WCR -- Filtering too Slowly after 900.0ml @16:27:57.21 SSV.dial :exhaust @16:27:57.98 Exhaust.select :open @16:27:58.09 SS.reconfigure :exhale @16:27:58.14 SS.seek 0ml @16:28:00.78 PSR.select :air @16:28:00.80 PSV.dial PSR @16:28:01.90 Pulling 0.5ml of PSR.flush @16:28:01.91 PSR.select :flush @16:28:02.02 PS.seek 0.5ml @16:28:07.02 Exhaust.select :close @16:28:07.13 Sampled 900.0ml @16:28:07.15 Drying puck before fixing for 20:00 @16:28:07.16 CBV.dial :waste2b @16:28:07.91 delay 1:00 @16:28:07.97 delay 5 seconds @16:28:12.99 PSR.select :air @16:28:13.10 PS.seek 2.5ml @16:28:34.31 delay 1 second @16:28:35.33 PBV.dial :waste1 @16:28:35.70 PTV.dial :mixing @16:28:36.45 PSV.dial :mixloop @16:28:37.50 PS.seek :empty @16:29:03.87 PSV.dial PSR @16:29:05.40 PSR.select :flush @16:29:05.52 PS.seek 1ml @16:29:07.93 CSV.dial :air @16:29:08.39 CS.reconfigure :fast @16:29:08.43 CS.seek 10ml @16:29:16.59 PSR.select :air @16:29:16.70 PS.seek 2.5ml @16:29:32.79 Pumping wcr puck from tube 4 up to 35psi... @16:29:32.81 CBV.dial SSV @16:29:32.87 PSV.dial PTV @16:29:33.62 PTV.dial :puck @16:29:34.10 CBV.dial between CSV and waste2b @16:29:34.36 PBV.dial :waste1 @16:29:34.38 PS.seek 0ml @16:29:35.07 CSV.dial between air and CTV @16:29:35.39 CS.seek 7.049ml @16:29:42.92 CTV.dial CSV,avoiding: :air @16:29:42.96 CSV.dial CTV @16:29:43.71 CS.reconfigure :pressurize @16:29:43.76 CS.seek 0ml @16:29:57.30 CBV.dial :waste2b @16:29:57.63 CS.reconfigure :default @16:29:57.68 CS.seek 0ml @16:30:00.71 PSV.dial PSR @16:30:01.57 PSR.select :flush @16:30:01.69 PS.seek 0.5ml @16:30:03.57 delay 1:00 @16:30:07.56 PSR.select :air @16:30:07.67 PS.seek 2.5ml @16:30:28.90 PSV.dial PTV @16:30:29.64 PBV.dial PSV @16:30:30.20 PS.seek 0ml @16:30:56.59 PSV.dial PBV @16:30:57.07 PS.seek 2.5ml @16:31:03.59 CTV.dial :air,avoiding: :intake @16:31:04.88 Fix #1 of 2 (with RNAlater) for 10:00 @16:31:04.90 CSV.dial CSR @16:31:05.39 Pulling 2ml of CSR.RNAlater @16:31:05.40 CSR.select :RNAlater @16:31:05.51 CS.seek 2ml @16:31:11.38 delay 5 seconds @16:31:16.40 CSR.select :air @16:31:16.51 CS.reconfigure :fast @16:31:16.56 CS.seek 10ml @16:31:23.40 PSV.dial :waste1 @16:31:24.14 PS.seek 0ml @16:31:36.29 Pumping wcr puck from tube 4 up to 35psi... @16:31:36.30 CBV.dial SSV @16:31:37.59 CBV.dial between CSV and waste2b @16:31:38.53 CSV.dial between air and CTV @16:31:39.14 CS.seek 6.971ml @16:31:46.79 CTV.dial CSV,avoiding: :air @16:31:46.81 CSV.dial CTV @16:31:48.18 CS.reconfigure :pressurize @16:31:48.23 CS.seek 0ml @16:31:50.52 PSV.dial PSR @16:31:50.99 PSR.select :air @16:31:51.02 PS.seek 2.5ml @16:32:02.40 delay 10:00 @16:32:17.40 PSV.dial PTV @16:32:18.25 PBV.dial PSV @16:32:18.27 PS.seek 0ml @16:32:44.62 PSV.dial PBV @16:32:45.08 PS.seek 2.5ml @16:33:11.46 PSV.dial :waste1 @16:33:12.20 PS.seek 0ml @16:33:38.52 PSV.dial PSR @16:33:38.99 PSR.select :flush @16:33:39.11 PS.seek 1ml @16:33:50.33 PSR.select :air @16:33:50.45 PS.seek 2.5ml @16:34:06.59 PSV.dial PTV @16:34:07.30 PTV.dial :puck @16:34:07.32 PBV.dial :waste1 @16:34:07.87 PS.seek 0ml @16:34:34.30 PSV.dial PSR @16:34:35.05 PSR.select :flush @16:34:35.17 PS.seek 0.5ml @16:34:41.11 PSR.select :air @16:34:41.23 PS.seek 2.5ml @16:35:02.43 PSV.dial PTV @16:35:03.18 PBV.dial PSV @16:35:03.73 PS.seek 0ml @16:35:30.11 PSV.dial PBV @16:35:30.70 PS.seek 2.5ml @16:35:57.11 PSV.dial :waste1 @16:35:57.87 PS.seek 0ml @16:36:24.39 PSV.dial PSR @16:36:24.85 PSR.select :air @16:36:24.87 PS.seek 2.5ml @16:36:51.24 PSV.dial PTV @16:36:51.97 PBV.dial PSV @16:36:51.99 PS.seek 0ml @16:37:18.55 PSV.dial PBV @16:37:19.02 PS.seek 2.5ml @16:37:45.39 PSV.dial :waste1 @16:37:46.13 PS.seek 0ml @16:38:12.46 Garaging FlushPuck in Processing Clamp @16:38:12.53 Elbow.reconfigure :tight @16:38:12.57 Elbow.seek PC @16:38:12.69 Elbow.reconfigure :loose @16:38:12.78 Forearm.seek PC @16:38:13.74 Hand.close @16:38:14.39 PC.seek :open @16:38:19.09 Forearm.seek :retract @16:38:20.04 Elbow.reconfigure :tight @16:38:20.09 Elbow.seek :garage @16:38:21.43 Elbow.reconfigure :loose @16:38:21.52 Forearm.reconfigure :tight @16:38:21.56 Forearm.seek :garage @16:38:22.51 Hand.open @16:38:23.19 Forearm.reconfigure :loose @16:38:23.24 Forearm.seek :retract @16:42:02.42 CBV.dial :waste2b @16:42:02.75 CS.reconfigure :default @16:42:02.79 CS.seek 0ml @16:42:07.65 delay 1:00 @16:43:07.68 CTV.dial :air,avoiding: :intake @16:43:08.99 Fix #2 of 2 (with RNAlater) for 10:00 @16:43:09.01 CSV.dial CSR @16:43:09.61 Pulling 2ml of CSR.RNAlater @16:43:09.62 CSR.select :RNAlater @16:43:09.73 CS.seek 2ml @16:43:15.71 delay 5 seconds @16:43:20.73 CSR.select :air @16:43:20.84 CS.reconfigure :fast @16:43:20.89 CS.seek 10ml @16:43:40.33 Pumping wcr puck from tube 4 up to 35psi... @16:43:40.35 CBV.dial SSV @16:43:41.63 CBV.dial between CSV and waste2b @16:43:42.58 CSV.dial between air and CTV @16:43:43.16 CS.seek 6.971ml @16:43:50.84 CTV.dial CSV,avoiding: :air @16:43:50.86 CSV.dial CTV @16:43:52.21 CS.reconfigure :pressurize @16:43:52.26 CS.seek 0ml @16:44:05.54 delay 10:00 @16:54:05.56 CBV.dial :waste2b @16:54:05.89 CS.reconfigure :default @16:54:05.94 CS.seek 0ml @16:54:11.88 delay 1:00 @16:55:11.91 CTV.dial :air,avoiding: :intake @16:55:13.27 Storing wcr puck in Collection Clamp to tube 3 @16:55:13.43 SC.reconfigure :tight @16:55:13.48 SC.seek 3 @16:55:14.13 SC.configure :limp @16:55:14.33 Elbow.reconfigure :tight @16:55:14.37 Elbow.seek 3 @16:55:16.38 Forearm.reconfigure :tight @16:55:16.42 Forearm.seek 3 + 450 counts @16:55:17.48 SE.reconfigure :pick @16:55:17.53 SE.seek :beamBreak @16:55:18.74 SE.configure :default @16:55:18.89 Elbow.reconfigure :limp @16:55:18.93 Forearm.reconfigure :loose @16:55:18.98 Forearm.seek 3 @16:55:19.26 Elbow.hold @16:55:19.33 Elbow.reconfigure :tight @16:55:19.43 SE.seek 28.09 Pucks below Top Plate @16:55:19.91 Forearm.force -20 @16:55:19.94 Forearm.seek 3 + 302 counts @16:55:20.23 Forearm.reconfigure :tight @16:55:20.27 Forearm.seek 3 + 450 counts @16:55:20.49 SE.seek 29.09 Pucks below Top Plate @16:55:21.07 Forearm.reconfigure :loose @16:55:21.11 Forearm.seek :retract @16:55:22.06 Elbow.seek CC @16:55:23.85 Elbow.reconfigure :loose @16:55:23.94 Forearm.seek CC @16:55:24.92 Hand.close @16:55:25.50 CC.seek :open @16:55:30.23 Forearm.seek :retract @16:55:31.20 Elbow.reconfigure :tight @16:55:31.25 Elbow.seek 3 @16:55:33.03 Elbow.reconfigure :limp @16:55:33.08 Forearm.seek 3 @16:55:34.04 Elbow.hold @16:55:34.11 Elbow.reconfigure :tight @16:55:34.16 Hand.open @16:55:34.86 Forearm.force -20 @16:55:34.89 Forearm.seek 3 + 316 counts @16:55:35.17 Forearm.reconfigure :tight @16:55:35.22 Forearm.seek 3 + 450 counts @16:55:35.47 SE.seek :down @16:55:36.23 SE.stop @16:55:36.59 Forearm.reconfigure :loose @16:55:36.64 Forearm.seek :retract @16:55:37.58 Starting finalPurge @16:55:37.59 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @16:55:37.77 Elbow.seek FlushPuck::Garage @16:55:39.61 Elbow.reconfigure :loose @16:55:39.70 Forearm.seek :garage @16:55:40.70 Hand.close @16:55:41.36 Forearm.seek :retract @16:55:42.34 Elbow.reconfigure :tight @16:55:42.38 Elbow.seek CC @16:55:45.31 Elbow.reconfigure :loose @16:55:45.40 Forearm.reconfigure :tight @16:55:45.44 Forearm.seek CC @16:55:46.62 CC.seek :closed @16:55:51.13 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.32 @16:55:51.19 Hand.open @16:55:51.81 Forearm.reconfigure :loose @16:55:51.85 Forearm.seek :retract @16:55:52.76 Flushing the Collection Valve::Manifold @16:55:52.88 CSV.dial CSR @16:55:53.36 Pulling 4.999ml of CSR.flush @16:55:53.37 CSR.select :flush @16:55:53.48 CS.seek 5ml @16:56:07.29 delay 5 seconds @16:56:12.31 CSR.select :air @16:56:12.46 CS.reconfigure :fast @16:56:12.50 CS.seek 7.499ml @16:56:19.16 CSV.dial :waste @16:56:20.42 CS.reconfigure :default @16:56:20.46 CS.seek 0ml @16:56:40.51 Flushing lower manifold's waste1 port @16:56:40.62 CSV.dial :flush @16:56:41.06 CS.reconfigure :fast @16:56:41.10 CS.seek 5ml @16:56:53.91 delay 5 seconds @16:56:58.93 CSV.dial :air @16:56:59.43 CS.seek 7ml @16:57:04.98 CSV.dial CTV @16:57:05.02
CTV.dial CSV @16:57:06.36 CBV.dial :waste1 @16:57:07.11 CS.seek 0ml @16:57:23.60 Flushing lower manifold's waste2 port @16:57:23.72 CSV.dial :flush @16:57:24.43 CS.seek 5ml @16:57:36.97 delay 5 seconds @16:57:41.99 CSV.dial :air @16:57:42.50 CS.seek 7ml @16:57:47.97 CSV.dial CTV @16:57:48.00
CTV.dial CSV @16:57:48.44 CBV.dial :waste2 @16:57:49.48 CS.seek 0ml @16:58:06.00 Flushing lower manifold's waste2b port @16:58:06.12 CSV.dial :flush @16:58:06.85 CS.seek 5ml @16:58:19.63 delay 5 seconds @16:58:24.65 CSV.dial :air @16:58:25.16 CS.seek 7ml @16:58:30.57 CSV.dial CTV @16:58:30.59
CTV.dial CSV @16:58:31.02 CBV.dial :waste2b @16:58:32.06 CS.seek 0ml @16:58:48.50 Disinfecting external Intake with kill @16:58:48.62 CSV.dial CSR @16:58:49.10 Pulling 8ml of CSR.kill @16:58:49.11 CSR.select :kill @16:58:49.23 CS.seek 8ml @16:59:09.01 delay 5 seconds @16:59:14.02 CSR.select :air @16:59:14.14 CS.seek 10ml @16:59:19.85 CSV.dial CBV @16:59:19.87
CBV.dial CSV @16:59:21.02 CTV.dial :intake @16:59:21.78 Intake.select :open @16:59:21.89 CS.seek 2ml @16:59:40.53 Intake.select :close @16:59:40.63 CTV.dial :air @16:59:41.38 CS.reconfigure :default @16:59:41.43 CS.seek 0ml @16:59:47.40 CSV.dial CSR @16:59:48.46 Pulling 5ml of CSR.flush @16:59:48.47 CSR.select :flush @16:59:48.58 CS.seek 5ml @17:00:02.19 delay 5 seconds @17:00:07.21 CSR.select :air @17:00:07.32 CS.seek 10ml @17:00:20.87 CSV.dial CBV @17:00:21.87 CS.seek 0ml @17:00:48.29 Garaging FlushPuck in Collection Clamp @17:00:48.35 Elbow.reconfigure :tight @17:00:48.40 Elbow.seek CC @17:00:48.79 Elbow.reconfigure :loose @17:00:48.88 Forearm.seek CC @17:00:49.85 Hand.close @17:00:50.49 CC.seek :open @17:00:55.11 Forearm.seek :retract @17:00:56.07 Elbow.reconfigure :tight @17:00:56.11 Elbow.seek :garage @17:00:58.98 Elbow.reconfigure :loose @17:00:59.07 Forearm.reconfigure :tight @17:00:59.11 Forearm.seek :garage @17:01:00.13 Hand.open @17:01:01.26 Forearm.reconfigure :loose @17:01:01.31 Forearm.seek :retract @17:01:02.29 Completed [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] begun 14:13:59 @17:01:02.34 Asleep after completing DA_WCR_032223 assays @17:01:02.36 GoodNews.email "{1 => 33,\n 2 => 33,\n 3 => 30,\n 4 => 3,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@16:53:10, 16% flow, 24.1C, 39% humidity, 15.0psia, 13.749V, 0.417A, 0.405A avg, 5.74W\nSampled 900.0ml",Subject: "Asleep after completing DA_WCR_032223 assays" @17:01:07.56 upsync @17:04:16.88UTC27-Mar-23
ESP2 v3.41 I2C Gateway v3.20 for core bus @17:04:19.55 Configuration for short puck jake 1/11/21 by brent@mbari.org /home/esp/esp2/type/shallow/jake/configure.rb @17:04:23.47 Polling Can every 10:00 @17:04:23.74 Cleanly exited at 16:26:16UTC27-Mar-23 @17:04:23.78 Loading /home/esp/esp2/mission/DA_WCR_032723.rb mission @17:04:23.84 Preparing to sleep until + 00:00:05 @17:04:23.85 GoodNews.email "Starting DA_WCR_032723 w/pucks in tube 4\n{1 => 33,\n 2 => 33,\n 3 => 30,\n 4 => 3,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@17:04:23, 16% flow, 24.6C, 41% humidity, 15.1psia, 13.773V, 0.321A, 0.309A avg, 4.42W",Subject: "DA_WCR_032723 begun" @17:04:23.90
Asleep til + 00:00:05 @17:04:23.92 Continuing at 17:04 @17:04:24.18 Gate.power :core,:OFF @17:04:24.20 Gate.power :raw,:OFF Shutdown @17:04:27.32 GoodNews.email "Then, in tube 4: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2/mission/DA_WCR_032723.rb:14\n{1 => 33,\n 2 => 33,\n 3 => 30,\n 4 => 3,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@17:04:23, 16% flow, 24.5C, 41% humidity, 15.1psia, 13.769V, 0.321A, 0.309A avg, 4.41W",Subject: "Asleep until 17:04" @17:04:28.81
Powering core bus @17:04:29.08 upsync @17:04:32.31 upsync @17:04:34.19
Gate.power :raw,:ON @17:04:34.91 CS.configure :default @17:04:35.00 PS.configure :default @17:04:35.09 SS.configure :default @17:04:35.18 CC.configure :default @17:04:35.27 PC.configure :default @17:04:35.37 Forearm.configure :loose @17:04:35.46 Elbow.configure :loose @17:04:35.56 SC.configure :loose @17:04:35.65 SE.configure :default @17:04:35.69 CH.coast @17:04:35.74 CH.configure :default @17:04:35.77 PH.coast @17:04:35.82 PH.configure :default @17:04:35.85 All dwarf controllers running firmware version 3.85 @17:04:35.95 Valve.reset @17:04:36.14 Intake.select :close @17:04:36.20 Exhaust.select :close @17:04:36.24 CTV.dial :air @17:04:36.32 PSV.dial :air @17:04:36.39 CSV.dial :waste1 @17:04:36.47
Forearm.reconfigure :loose @17:04:36.51 Forearm.home.seek :retract @17:04:36.86 SSV.dial :spit,avoiding: :exhaust @17:04:37.23 Hand.close @17:04:37.26
Elbow.jog 1500 @17:04:37.41 PS.reconfigure :default @17:04:37.46 PS.home.seek 0ml @17:04:37.68 CS.reconfigure :fast @17:04:37.70 SS.reconfigure :default @17:04:37.79 CS.home.seek 0ml @17:04:37.82 SS.home.seek 0ml @17:04:37.93
Elbow.reconfigure :loose @17:04:37.97 Elbow.home.seek :clear @17:04:41.18 SE.reconfigure :default @17:04:41.22 SE.home.seek :down @17:04:41.86 SC.reconfigure :tight @17:04:41.90 SC.home.seek :initial @17:04:45.68 PS.stop @17:05:10.21
SC.configure :limp @17:05:10.26 Forearm.seek :retract @17:05:10.31 Elbow.seek :hammer @17:05:11.34 Forearm.reconfigure :tight @17:05:11.38 Forearm.seek retracted @17:05:11.73 CC.reconfigure :default @17:05:11.74 PC.reconfigure :default @17:05:11.83
CC.home.seek :open @17:05:11.85 PC.home.seek :open @17:05:15.38
Phase [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] @17:05:15.39 Notice.email "At 17:05:15 began in tube 4: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2/mission/DA_WCR_032723.rb:14\n{1 => 33,\n 2 => 33,\n 3 => 30,\n 4 => 3,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@17:05:15, 17% flow, 24.6C, 41% humidity, 15.1psia, 13.734V, 0.460A, 0.447A avg, 6.31W",Subject: "Started DA_WCR_032723 phase initialPurge; da [1000, 1000] #da and wsr are run simultanelously" @17:05:15.41
Starting initialPurge @17:05:15.44 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @17:05:15.50 Hand.open @17:05:16.23 Elbow.reconfigure :tight @17:05:16.29 Elbow.seek FlushPuck::Garage @17:05:18.07 Elbow.reconfigure :loose @17:05:18.15 Forearm.reconfigure :loose @17:05:18.19 Forearm.seek :garage @17:05:19.14 Hand.close @17:05:19.93 Forearm.seek :retract @17:05:20.54 upsync @17:05:20.89
Elbow.reconfigure :tight @17:05:20.94 Elbow.seek CC @17:05:23.86 Elbow.reconfigure :loose @17:05:23.95 Forearm.reconfigure :tight @17:05:24.00 Forearm.seek CC @17:05:25.19 CC.seek :closed @17:05:29.74 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.27 @17:05:29.81 Hand.open @17:05:30.43 Forearm.reconfigure :loose @17:05:30.47 Forearm.seek :retract @17:05:31.44 Flushing the Collection Valve::Manifold @17:05:31.55 CSV.dial CSR @17:05:32.86 Pulling 5ml of CSR.flush @17:05:32.87 CSR.select :flush @17:05:32.98 CS.reconfigure :default @17:05:33.02 CS.seek 5ml @17:05:46.57 delay 5 seconds @17:05:51.59 CSR.select :air @17:05:51.75 CS.reconfigure :fast @17:05:51.79 CS.seek 7.5ml @17:05:58.44 CSV.dial :waste @17:05:59.70 CS.reconfigure :default @17:05:59.74 CS.seek 0ml @17:06:19.70 Purging external Intake @17:06:19.81 CSV.dial :flush @17:06:20.25 CS.reconfigure :fast @17:06:20.29 CS.seek 10ml @17:06:44.51 delay 5 seconds @17:06:49.53 CSV.dial CBV @17:06:49.56
CBV.dial CSV @17:06:50.30 CTV.dial :intake @17:06:51.07 Intake.select :open @17:06:51.17 CS.seek 0ml @17:07:14.38 Intake.select :close @17:07:14.48 CTV.dial :air @17:07:15.24 Flushing upper manifold @17:07:15.35 CSV.dial :flush @17:07:16.09 CS.seek 5ml @17:07:28.63 delay 5 seconds @17:07:33.65 CSV.dial :air @17:07:34.14 CS.seek 7ml @17:07:39.64 CSV.dial CBV @17:07:39.65
CBV.dial CSV @17:07:40.68 CTV.dial :air @17:07:40.70 CS.seek 0ml @17:07:57.13 delay 7 seconds @17:08:04.16 CS.seek 3ml @17:08:12.13 CSV.dial :waste @17:08:12.57 CS.seek 0ml @17:08:20.42 CSV.dial :flush @17:08:20.86 CS.seek 3ml @17:08:28.82 delay 5 seconds @17:08:33.83 CSV.dial :air @17:08:34.33 CS.seek 5ml @17:08:39.75 CSV.dial :air @17:08:39.77 CS.seek 0ml @17:08:52.00 delay 7 seconds @17:08:59.03 CS.seek 3ml @17:09:07.00 CSV.dial :waste @17:09:07.73 CS.seek 0ml @17:09:15.57 Garaging FlushPuck in Collection Clamp @17:09:15.63 Elbow.reconfigure :tight @17:09:15.68 Elbow.seek CC @17:09:15.94 Elbow.reconfigure :loose @17:09:16.02 Forearm.seek CC @17:09:16.99 Hand.close @17:09:17.62 CC.seek :open @17:09:22.23 Forearm.seek :retract @17:09:23.26 Elbow.reconfigure :tight @17:09:23.30 Elbow.seek :garage @17:09:26.17 Elbow.reconfigure :loose @17:09:26.26 Forearm.reconfigure :tight @17:09:26.30 Forearm.seek :garage @17:09:27.29 Hand.open @17:09:28.42 Forearm.reconfigure :loose @17:09:28.47 Forearm.seek :retract @17:09:29.43 da sampling at most 1000ml, wcr at most 1000ml @17:09:29.47 Loading sh1 puck from tube 4 into Collection Clamp @17:09:29.48 DAfans.email "Started da in tube 4 at 17:09:29UTC27-Mar-23\n{1 => 33,\n 2 => 33,\n 3 => 30,\n 4 => 3,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@17:05:15, 17% flow, 24.6C, 41% humidity, 15.1psia, 13.734V, 0.460A, 0.447A avg, 6.31W",Subject: "da sampling at most 1000ml, wcr at most 1000ml" @17:09:29.72
SC.reconfigure :tight @17:09:29.77 SC.seek 4 @17:09:29.86 SC.configure :limp @17:09:30.08 Elbow.reconfigure :tight @17:09:30.13 Elbow.seek 4 @17:09:32.03 Forearm.reconfigure :tight @17:09:32.07 Forearm.seek 4 + 450 counts @17:09:33.20 SE.reconfigure :pick @17:09:33.28 SE.seek :beamBreak @17:09:34.75 upsync @17:09:36.24
SE.configure :default @17:09:36.39 Elbow.seek 4 @17:09:36.56 Forearm.seek 4 + 300 counts @17:09:36.80 Forearm.reconfigure :loose @17:09:36.85 Hand.close @17:09:37.51 SE.seek :down @17:09:37.70
Forearm.seek :retract @17:09:38.64 Elbow.seek CC @17:09:40.14 SE.stop @17:09:40.26
Elbow.reconfigure :loose @17:09:40.35 Forearm.reconfigure :tight @17:09:40.39 Forearm.seek CC @17:09:41.64 CC.seek :closed @17:09:46.16 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.14 @17:09:46.17 Hand.open @17:09:46.80 Forearm.reconfigure :loose @17:09:46.84 Forearm.seek :retract @17:09:47.79 collectSample 1000ml w/bubble point of 24psi @17:09:47.80 Priming 7ml @17:09:47.81 Intake.select :close @17:09:47.82 Exhaust.select :close @17:09:47.84 SS.hold @17:09:48.03 Calibrating pressure and temperature sensors @17:09:48.06 CTV.dial :air @17:09:48.07 SSV.dial :spit,avoiding: :exhaust @17:09:48.09 SS.reconfigure :fast @17:09:48.13 SS.seek 5ml @17:09:51.02 SSV.dial CBV @17:09:51.04
CBV.dial SSV @17:09:52.35 delay 3.8 seconds @17:09:56.26 SSV.dial :spit @17:09:57.57 SS.seek 0ml @17:10:00.64 SSV.dial CBV @17:10:01.91 CTV.dial :intake @17:10:02.68 SSV.dial CBV @17:10:02.70
CBV.dial SSV @17:10:02.71 Allowing 40s for 7.0ml of remaining 7.0ml @17:10:02.72 Intake.select :open @17:10:04.92 Intake @15.2psia, vacuum from 9.7 to 12.7psi @17:10:04.93 SS.reconfigure :inhaling 2.20ml/s @17:10:04.97 SS.seek 7ml @17:10:10.81 Stroke completed -- waiting for pressure to equalize ... @17:10:10.82 SS.reconfigure :exhale @17:10:10.86 SS.hold @17:10:12.92 Intake.select :close @17:10:13.06 SSV.dial :exhaust @17:10:13.81 Exhaust.select :open @17:10:13.93 SS.seek 0ml @17:10:17.86 Exhaust.select :close @17:10:17.96 Sampling 1000.0ml @17:10:17.97 Intake.select :close @17:10:17.98 Exhaust.select :close @17:10:17.99 SS.reconfigure :default @17:10:18.03 SS.hold @17:10:18.21 CTV.dial :intake @17:10:18.22 SSV.dial CBV @17:10:18.25
CBV.dial SSV @17:10:19.00 Allowing 130s for 25.0ml of remaining 1000.0ml @17:10:19.01 Intake.select :open @17:10:21.16 Intake @15.3psia, vacuum from 9.8 to 12.8psi @17:10:21.18 SS.reconfigure :inhaling 2.20ml/s @17:10:21.22 SS.seek 25ml @17:10:31.60 Vacuum too high -- waiting up to 40 seconds @17:10:31.61 SS.reconfigure :exhale @17:10:31.65 SS.hold @17:10:32.39 Intake @11.8psia, vacuum from 6.3 to 9.3psi @17:10:32.41 SS.reconfigure :inhaling 1.98ml/s @17:10:32.45 SS.seek 25ml @17:10:35.39 Vacuum too high -- waiting up to 40 seconds @17:10:35.40 SS.reconfigure :exhale @17:10:35.44 SS.hold @17:10:41.75 Intake @13.4psia, vacuum from 7.9 to 10.9psi @17:10:41.76 SS.reconfigure :inhaling 1.79ml/s @17:10:41.81 SS.seek 25ml @17:10:46.33 Stroke completed -- waiting for pressure to equalize ... @17:10:46.34 SS.reconfigure :exhale @17:10:46.38 SS.hold @17:10:53.73 Intake.select :close @17:10:53.86 SSV.dial :exhaust @17:10:54.58 Exhaust.select :open @17:10:54.68 SS.seek 0ml @17:11:03.50 Exhaust.select :close @17:11:03.60 SSV.dial CBV @17:11:03.62
CBV.dial SSV @17:11:04.39 Allowing 130s for 25.0ml of remaining 975.0ml @17:11:04.40 Intake.select :open @17:11:06.55 Intake @14.5psia, vacuum from 9.0 to 12.0psi @17:11:06.57 SS.reconfigure :inhaling 1.79ml/s @17:11:06.61 SS.seek 25ml @17:11:15.04 Vacuum too high -- waiting up to 40 seconds @17:11:15.05 SS.reconfigure :exhale @17:11:15.09 SS.hold @17:11:22.69 Intake @13.6psia, vacuum from 8.1 to 11.1psi @17:11:22.71 SS.reconfigure :inhaling 1.61ml/s @17:11:22.75 SS.seek 25ml @17:11:27.60 Vacuum too high -- waiting up to 40 seconds @17:11:27.61 SS.reconfigure :exhale @17:11:27.65 SS.hold @17:11:40.39 Intake @14.1psia, vacuum from 8.6 to 11.6psi @17:11:40.40 SS.reconfigure :inhaling 1.45ml/s @17:11:40.45 SS.seek 25ml @17:11:46.16 Vacuum too high -- waiting up to 40 seconds @17:11:46.17 SS.reconfigure :exhale @17:11:46.21 SS.hold @17:12:04.76 Intake @14.2psia, vacuum from 8.7 to 11.7psi @17:12:04.78 SS.reconfigure :inhaling 1.30ml/s @17:12:04.82 SS.seek 25ml @17:12:07.01 Stroke completed -- waiting for pressure to equalize ... @17:12:07.02 SS.reconfigure :exhale @17:12:07.06 SS.hold @17:12:25.27 Intake.select :close @17:12:25.41 SSV.dial :exhaust @17:12:26.15 Exhaust.select :open @17:12:26.26 SS.seek 0ml @17:12:35.09 Exhaust.select :close @17:12:35.19 SSV.dial CBV @17:12:35.21
CBV.dial SSV @17:12:35.97 Allowing 130s for 25.0ml of remaining 950.0ml @17:12:35.98 Intake.select :open @17:12:38.14 Intake @14.8psia, vacuum from 9.3 to 12.3psi @17:12:38.15 SS.reconfigure :inhaling 1.30ml/s @17:12:38.20 SS.seek 25ml @17:12:46.88 Vacuum too high -- waiting up to 40 seconds @17:12:46.89 SS.reconfigure :exhale @17:12:46.93 SS.hold @17:13:05.25 Intake @14.5psia, vacuum from 9.0 to 12.0psi @17:13:05.26 SS.reconfigure :inhaling 1.17ml/s @17:13:05.30 SS.seek 25ml @17:13:11.35 Vacuum too high -- waiting up to 40 seconds @17:13:11.36 SS.reconfigure :exhale @17:13:11.40 SS.hold @17:13:39.92 Intake @14.7psia, vacuum from 9.2 to 12.2psi @17:13:39.93 SS.reconfigure :inhaling 1.06ml/s @17:13:39.98 SS.seek 25ml @17:13:46.13 Vacuum too high -- waiting up to 40 seconds @17:13:46.14 SS.reconfigure :exhale @17:13:46.18 SS.hold @17:14:24.88 SS.reconfigure :inhaling 0.95ml/s @17:14:24.92 SS.seek 25ml @17:14:31.34 Vacuum too high -- waiting up to 40 seconds @17:14:31.35 SS.reconfigure :exhale @17:14:31.40 SS.hold @17:14:45.99 SS.reconfigure :default @17:14:46.03 SS.hold @17:14:46.14 Intake.select :close @17:14:46.28 ShallowSampler::Clogged in MAIN -- Filtering too Slowly after 74.7ml @17:14:46.29 SSV.dial :exhaust @17:14:47.03 Exhaust.select :open @17:14:47.13 SS.reconfigure :exhale @17:14:47.17 SS.seek 0ml @17:14:56.10 Exhaust.select :close @17:14:56.21 Sampled 74.7ml @17:14:56.22 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @17:14:56.23 Intake.select :close @17:14:56.28 CSV.dial :waste @17:14:56.34 CS.seek 0ml @17:14:56.43 CSV.dial :air @17:14:56.45 SSV.dial CBV,avoiding: :exhaust @17:14:56.49
CBV.dial SSV @17:14:57.21 CS.seek 10ml @17:14:58.34
CTV.dial :air @17:14:59.12 delay 15 seconds @17:15:14.14 SSV.dial :spit,avoiding: :exhaust @17:15:15.44 CTV.dial CSV,avoiding: :intake @17:15:15.48
SS.seek 0ml @17:15:15.58 SSV.dial CBV,avoiding: :exhaust @17:15:21.49 CBV.dial between SSV and waste2 @17:15:21.87 CTV.dial CSV,avoiding: :air @17:15:21.90
CSV.dial CTV @17:15:22.33 CS.reconfigure :pressurize @17:15:22.37 CS.seek 0ml @17:15:41.02 BubblePoint: Intake @39.4psia, Outlet @15.1psia @17:15:41.03 CBV.dial SSV @17:15:41.36 SS.reconfigure :default @17:15:41.40 SS.seek 10ml @17:15:47.18 delay 15 seconds @17:16:02.30 Evacuated: Intake @17.3psia, Outlet @9.7psia @17:16:02.31 SSV.dial :spit,avoiding: :exhaust @17:16:03.62 SS.reconfigure :exhale @17:16:03.67 SS.seek 0ml @17:16:09.08 SS.reconfigure :fast @17:16:09.12 SS.seek 5ml @17:16:12.05 SSV.dial CBV,avoiding: :exhaust @17:16:12.06
CBV.dial SSV @17:16:13.39 CTV.dial :air,avoiding: :intake @17:16:14.73 CSV.dial CTV @17:16:14.76
SS.seek 10ml @17:16:15.77 CSV.dial :air @17:16:16.22 CS.reconfigure :fast @17:16:16.26 CS.seek 0ml @17:16:17.26
SSV.dial :spit,avoiding: :exhaust @17:16:18.56 SS.reconfigure :exhale @17:16:18.60 SS.seek 0ml @17:16:23.62 CS.stop @17:16:23.89
SS.reconfigure :fast @17:16:23.93 SS.seek 5ml @17:16:26.81 SSV.dial CBV,avoiding: :exhaust @17:16:26.83
CBV.dial SSV @17:16:29.14 SSV.dial :spit,avoiding: :exhaust @17:16:30.44 SS.reconfigure :default @17:16:30.48 SS.seek 0ml @17:16:33.80 SS.stop @17:16:33.84 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @17:16:33.85 Intake.select :close @17:16:33.92 CSV.dial :waste @17:16:34.03 SSV.dial CBV,avoiding: :exhaust @17:16:34.05
CBV.dial SSV @17:16:34.66 CS.seek 0ml @17:16:34.72 CSV.dial :air @17:16:35.35
CTV.dial :air @17:16:35.37 delay 15 seconds @17:16:35.57 CS.seek 10ml @17:16:50.38
SSV.dial :spit,avoiding: :exhaust @17:16:51.70 CTV.dial CSV,avoiding: :intake @17:16:51.73
SS.reconfigure :exhale @17:16:51.82 SS.seek 0ml @17:16:52.23 SSV.dial CBV,avoiding: :exhaust @17:16:59.64 CBV.dial between SSV and waste2 @17:17:00.03 CTV.dial CSV,avoiding: :air @17:17:00.05
CSV.dial CTV @17:17:00.50 CS.reconfigure :pressurize @17:17:00.54 CS.seek 0ml @17:17:21.71 BubblePoint: Intake @38.4psia, Outlet @14.3psia @17:17:21.72 CBV.dial SSV @17:17:22.05 SS.reconfigure :default @17:17:22.09 SS.seek 10ml @17:17:27.77 delay 15 seconds @17:17:42.89 Evacuated: Intake @18.4psia, Outlet @11.9psia @17:17:42.90 SSV.dial :spit,avoiding: :exhaust @17:17:44.18 SS.reconfigure :exhale @17:17:44.23 SS.seek 0ml @17:17:49.61 SS.reconfigure :fast @17:17:49.65 SS.seek 5ml @17:17:52.55 SSV.dial CBV,avoiding: :exhaust @17:17:52.56
CBV.dial SSV @17:17:53.87 CTV.dial :air,avoiding: :intake @17:17:55.20 CSV.dial CTV @17:17:55.23
SS.seek 10ml @17:17:56.23 CSV.dial :air @17:17:56.69 CS.reconfigure :fast @17:17:56.73 CS.seek 0ml @17:17:57.82
SSV.dial :spit,avoiding: :exhaust @17:17:59.14 SS.reconfigure :exhale @17:17:59.18 SS.seek 0ml @17:18:02.09 CS.stop @17:18:04.31
SS.reconfigure :fast @17:18:04.35 SS.seek 5ml @17:18:07.24 SSV.dial CBV,avoiding: :exhaust @17:18:07.75
CBV.dial SSV @17:18:10.03 SSV.dial :spit,avoiding: :exhaust @17:18:11.34 SS.reconfigure :default @17:18:11.38 SS.seek 0ml @17:18:14.72 SS.stop @17:18:14.76 Lysing: Adding 2.0ml AqMeOH @17:18:14.77 CBV.dial between CSV and waste1,avoiding: :DBV @17:18:16.25 CTV.dial CSV @17:18:17.56 CSV.dial CSR @17:18:18.34 Pulling 1ml of CSR.AqMeOH @17:18:18.35 CSR.select :AqMeOH @17:18:18.46 CS.reconfigure :default @17:18:18.50 CS.seek 1ml @17:18:21.96 delay 5 seconds @17:18:26.98 CSR.select :air @17:18:27.13 CS.seek 6.001ml @17:18:40.93 delay 2 seconds @17:18:42.95 CSV.dial :waste2 @17:18:43.39 CS.reconfigure :fast @17:18:43.43 CS.seek 0ml @17:18:57.62 CSV.dial CSR @17:18:58.11 Pulling 2ml of CSR.AqMeOH @17:18:58.12 CSR.select :AqMeOH @17:18:58.22 CS.reconfigure :default @17:18:58.26 CS.seek 2ml @17:19:04.28 delay 5 seconds @17:19:09.29 CSR.select :air @17:19:09.44 CS.seek 5ml @17:19:17.87 delay 2 seconds @17:19:19.88 CSV.dial CTV @17:19:20.33 CS.seek 1ml @17:19:31.44 Before lysing Collection Puck Heater at 24.1C/39.4psia @17:19:31.46 Heating Puck.from 4, :sh1 in Collection Clamp to 70C for 10:00 @17:19:31.47 CH.seek 70C,8:00 @17:21:16.19 CH.hold 10:00,70C @17:21:16.38 Begun lysing Collection Puck Heater at 68C/45.6psia @17:31:16.38 Ended lysing Collection Puck Heater at 70C/43.5psia @17:31:16.40 CS.seek 0ml @17:31:20.02 CTV.dial between spare and air,avoiding: :intake @17:31:21.03 CSV.dial :air @17:31:21.47 CS.seek 5ml @17:31:35.04 CSV.dial CTV @17:31:35.48 delay 2 seconds @17:31:37.50 CSV.dial :air @17:31:37.95 delay 2 seconds @17:31:39.96 CSV.dial CTV @17:31:40.41 delay 2 seconds @17:31:42.42 CSV.dial :air @17:31:42.87 delay 2 seconds @17:31:44.89 CS.seek 0ml @17:31:58.62 Puck @71.6psia after final lysis pressurization @17:31:58.63 Recovering Lysate into Collection syringe @17:31:58.64 CSV.dial CBV @17:31:59.64 CBV.dial CSV @17:31:59.99 CS.seek 4.999ml @17:32:13.54 delay 5 seconds @17:32:18.56 CTV.dial :air @17:32:19.08 CS.seek 6.999ml @17:32:25.23 delay 5 seconds @17:32:30.25 CSV.dial :air2 @17:32:30.69 CS.seek 10ml @17:32:39.26 CSV.dial between air and CTV,avoiding: CBV @17:32:40.41 Lysing Complete @17:32:40.47 Storing sh1 puck in Collection Clamp to tube 3 @17:32:40.62 SC.reconfigure :tight @17:32:40.67 SC.seek 3 @17:32:44.28 SC.configure :limp @17:32:44.96 Elbow.reconfigure :tight @17:32:45.02 Elbow.seek 3 @17:32:46.83 Forearm.reconfigure :tight @17:32:46.87 Forearm.seek 3 + 450 counts @17:32:47.88 SE.reconfigure :pick @17:32:47.93 SE.seek :beamBreak @17:32:49.08 SE.configure :default @17:32:49.22 Elbow.reconfigure :limp @17:32:49.27 Forearm.reconfigure :loose @17:32:49.31 Forearm.seek 3 @17:32:49.55 Elbow.hold @17:32:49.61 Elbow.reconfigure :tight @17:32:49.70 SE.seek 29.1 Pucks below Top Plate @17:32:50.18 Forearm.force -20 @17:32:50.21 Forearm.seek 3 + 288 counts @17:32:50.49 Forearm.reconfigure :tight @17:32:50.54 Forearm.seek 3 + 450 counts @17:32:50.78 SE.seek 30.1 Pucks below Top Plate @17:32:51.32 Forearm.reconfigure :loose @17:32:51.36 Forearm.seek :retract @17:32:52.29 Elbow.seek CC @17:32:53.96 Elbow.reconfigure :loose @17:32:54.05 Forearm.seek CC @17:32:55.00 Hand.close @17:32:55.60 CC.seek :open @17:33:00.32 Forearm.seek :retract @17:33:01.29 Elbow.reconfigure :tight @17:33:01.33 Elbow.seek 3 @17:33:03.11 Elbow.reconfigure :limp @17:33:03.15 Forearm.seek 3 @17:33:04.17 Elbow.hold @17:33:04.23 Elbow.reconfigure :tight @17:33:04.28 Hand.open @17:33:04.95 Forearm.force -20 @17:33:04.99 Forearm.seek 3 + 313 counts @17:33:05.26 Forearm.reconfigure :tight @17:33:05.31 Forearm.seek 3 + 450 counts @17:33:05.55 SE.seek :down @17:33:06.18 SE.stop @17:33:06.67
Forearm.reconfigure :loose @17:33:06.71 Forearm.seek :retract @17:33:07.62 Tranferring DA @17:33:07.63 Beginning samplePassC @17:33:07.64 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @17:33:07.82 Elbow.seek FlushPuck::Garage @17:33:09.68 Elbow.reconfigure :loose @17:33:09.77 Forearm.seek :garage @17:33:10.74 Hand.close @17:33:11.46 Forearm.seek :retract @17:33:12.43 Elbow.reconfigure :tight @17:33:12.47 Elbow.seek CC @17:33:15.46 Elbow.reconfigure :loose @17:33:15.55 Forearm.reconfigure :tight @17:33:15.60 Forearm.seek CC @17:33:16.85 CC.seek :closed @17:33:21.35 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.40 @17:33:21.42 Hand.open @17:33:22.03 Forearm.reconfigure :loose @17:33:22.07 Forearm.seek :retract @17:33:23.08 PSV.dial PSR @17:33:24.43 Pulling 1.5ml of PSR.flush @17:33:24.44 PSR.select :flush @17:33:24.56 PS.seek 1.5ml @17:33:40.65 delay 5 seconds @17:33:45.67 PSV.dial :waste1 @17:33:46.12 PS.seek 0ml @17:34:02.21 PSV.dial PSR @17:34:03.15 PSR.select :air @17:34:03.26 PS.seek 2.5ml @17:34:29.62 PRV.dial CBV,avoiding: :ab2 @17:34:29.64
CBV.dial PRV @17:34:31.29 PSV.dial :mixloop @17:34:32.62 CTV.dial :air,avoiding: :intake @17:34:32.63 PTV.dial :PRVmixing @17:34:33.93 PS.seek 0ml @17:35:00.30 PSV.dial :air @17:35:00.87 PS.seek 2.5ml @17:35:27.36 PSV.dial :mixloop @17:35:27.81 PS.seek 0ml @17:35:54.17 PSV.dial :air @17:35:54.63 PS.seek 2.5ml @17:36:21.05 PSV.dial :mixloop @17:36:21.51 PS.seek 0ml @17:36:47.99 Garaging FlushPuck in Collection Clamp @17:36:48.06 Elbow.reconfigure :tight @17:36:48.10 Elbow.seek CC @17:36:48.54 Elbow.reconfigure :loose @17:36:48.63 Forearm.seek CC @17:36:49.64 Hand.close @17:36:50.22 CC.seek :open @17:36:54.83 Forearm.seek :retract @17:36:55.81 Elbow.reconfigure :tight @17:36:55.86 Elbow.seek :garage @17:36:58.77 Elbow.reconfigure :loose @17:36:58.86 Forearm.reconfigure :tight @17:36:58.90 Forearm.seek :garage @17:36:59.94 Hand.open @17:37:00.65 Forearm.reconfigure :loose @17:37:00.69 Forearm.seek :retract @17:37:01.65 PSV.dial :mixloop @17:37:01.67 PTV.dial :PRVmixing @17:37:01.68 PRV.dial CBV @17:37:01.70
CBV.dial PRV @17:37:01.72 CTV.dial :air,avoiding: :intake @17:37:01.74 Loading lyfil puck from tube 4 into Collection Clamp @17:37:01.95 SC.reconfigure :tight @17:37:02.00 SC.seek 4 @17:37:05.60 SC.configure :limp @17:37:05.79 Elbow.reconfigure :tight @17:37:05.83 Elbow.seek 4 @17:37:07.70 Forearm.reconfigure :tight @17:37:07.75 Forearm.seek 4 + 450 counts @17:37:08.78 SE.reconfigure :pick @17:37:08.83 SE.seek :beamBreak @17:37:11.82 SE.configure :default @17:37:11.96 Elbow.seek 4 @17:37:12.12 Forearm.seek 4 + 300 counts @17:37:12.39 Forearm.reconfigure :loose @17:37:12.43 Hand.close @17:37:13.11 SE.seek :down @17:37:13.28
Forearm.seek :retract @17:37:14.24 Elbow.seek CC @17:37:15.84 Elbow.reconfigure :loose @17:37:15.85 SE.stop @17:37:15.99
Forearm.reconfigure :tight @17:37:16.03 Forearm.seek CC @17:37:17.21 CC.seek :closed @17:37:21.76 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.14 @17:37:21.77 Hand.open @17:37:22.41 Forearm.reconfigure :loose @17:37:22.92 Forearm.seek :retract @17:37:23.86 CTV.dial CSV,avoiding: :intake @17:37:25.18 CSV.dial CTV @17:37:25.61 PS.seek 2.5ml @17:37:52.05 CS.seek 4.5ml @17:38:06.91 delay 8 seconds @17:38:14.93 PSV.dial :air @17:38:15.39 delay 2 seconds @17:38:17.41 PSV.dial :waste2 @17:38:18.12 PS.seek 0ml @17:38:44.42 CBV.dial between PRV and waste1 @17:38:44.74 CSV.dial :air @17:38:45.18 CS.seek 10ml @17:39:00.13 CSV.dial CTV @17:39:00.57 CS.seek 0ml @17:39:27.25 CBV.dial :waste1 @17:39:27.54 delay 5 seconds @17:39:32.56 CTV.dial :air,avoiding: :intake @17:39:33.94 Storing lyfil puck in Collection Clamp to tube 3 @17:39:34.09 SC.reconfigure :tight @17:39:34.14 SC.seek 3 @17:39:37.79 SC.configure :limp @17:39:37.98 Elbow.reconfigure :tight @17:39:38.02 Elbow.seek 3 @17:39:39.82 Forearm.reconfigure :tight @17:39:39.87 Forearm.seek 3 + 450 counts @17:39:40.90 SE.reconfigure :pick @17:39:40.95 SE.seek :beamBreak @17:39:42.01 SE.configure :default @17:39:42.15 Elbow.reconfigure :limp @17:39:42.19 Forearm.reconfigure :loose @17:39:42.23 Forearm.seek 3 @17:39:42.51 Elbow.hold @17:39:42.57 Elbow.reconfigure :tight @17:39:42.66 SE.seek 30.09 Pucks below Top Plate @17:39:43.13 Forearm.force -20 @17:39:43.16 Forearm.seek 3 + 311 counts @17:39:43.44 Forearm.reconfigure :tight @17:39:43.48 Forearm.seek 3 + 450 counts @17:39:43.69 SE.seek 31.09 Pucks below Top Plate @17:39:44.37 Forearm.reconfigure :loose @17:39:44.42 Forearm.seek :retract @17:39:45.34 Elbow.seek CC @17:39:47.04 Elbow.reconfigure :loose @17:39:47.12 Forearm.seek CC @17:39:48.13 Hand.close @17:39:48.72 CC.seek :open @17:39:53.43 Forearm.seek :retract @17:39:54.39 Elbow.reconfigure :tight @17:39:54.43 Elbow.seek 3 @17:39:56.21 Elbow.reconfigure :limp @17:39:56.26 Forearm.seek 3 @17:39:57.33 Elbow.hold @17:39:57.39 Elbow.reconfigure :tight @17:39:57.43 Hand.open @17:39:58.20 Forearm.force -20 @17:39:58.23 Forearm.seek 3 + 299 counts @17:39:58.52 Forearm.reconfigure :tight @17:39:58.56 Forearm.seek 3 + 450 counts @17:39:58.84 SE.seek :down @17:39:59.47 SE.stop @17:39:59.96
Forearm.reconfigure :loose @17:40:00.00 Forearm.seek :retract @17:40:00.92 PSV.dial :air @17:40:01.65 PS.seek 2.5ml @17:40:27.95 delay 1 second @17:40:28.96 PSV.dial :mixloop @17:40:29.40 delay 1 second @17:40:30.42 PSV.dial :air @17:40:31.34 delay 1 second @17:40:32.36 PSV.dial :mixloop @17:40:32.83 delay 1 second @17:40:33.84 PSV.dial :air @17:40:34.30 delay 1 second @17:40:35.32 PS.seek 0ml @17:41:01.70 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @17:41:01.87 Elbow.seek FlushPuck::Garage @17:41:03.67 Elbow.reconfigure :loose @17:41:03.76 Forearm.seek :garage @17:41:04.73 Hand.close @17:41:05.45 Forearm.seek :retract @17:41:06.42 Elbow.reconfigure :tight @17:41:06.47 Elbow.seek PC @17:41:07.91 Elbow.reconfigure :loose @17:41:08.00 Forearm.reconfigure :tight @17:41:08.04 Forearm.seek PC @17:41:09.23 PC.seek :closed @17:41:13.86 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.57 @17:41:13.92 Hand.open @17:41:14.65 Forearm.reconfigure :loose @17:41:14.69 Forearm.seek :retract @17:41:15.64 PRV.dial :air @17:41:16.07 PSV.dial :mixloop @17:41:16.61 PS.seek 0.3ml @17:41:20.73 PSV.dial PSR @17:41:21.80 Pulling 0.4ml of PSR.air @17:41:21.81 PSR.select :air @17:41:21.83 PS.seek 0.7ml @17:41:26.70 delay 3 seconds @17:41:29.71 PSR.select :air @17:41:29.74 PS.seek 2.5ml @17:41:48.89 delay 3 seconds @17:41:51.90 PSV.dial :mixloop @17:41:52.92 PTV.dial :mixing @17:41:53.64 PBV.dial :waste2 @17:41:54.56 PS.seek 0ml @17:42:20.89 Garaging FlushPuck in Processing Clamp @17:42:20.96 Elbow.reconfigure :tight @17:42:21.00 Elbow.seek PC @17:42:21.11 Elbow.reconfigure :loose @17:42:21.20 Forearm.seek PC @17:42:22.17 Hand.close @17:42:22.72 PC.seek :open @17:42:27.43 Forearm.seek :retract @17:42:28.36 Elbow.reconfigure :tight @17:42:28.40 Elbow.seek :garage @17:42:29.70 Elbow.reconfigure :loose @17:42:29.79 Forearm.reconfigure :tight @17:42:29.84 Forearm.seek :garage @17:42:30.82 Hand.open @17:42:31.52 Forearm.reconfigure :loose @17:42:31.56 Forearm.seek :retract @17:42:32.51 Loading da puck from tube 4 into Processing Clamp @17:42:32.73 SC.reconfigure :tight @17:42:32.77 SC.seek 4 @17:42:36.41 SC.configure :limp @17:42:36.60 Elbow.reconfigure :tight @17:42:36.64 Elbow.seek 4 @17:42:38.48 Forearm.reconfigure :tight @17:42:38.53 Forearm.seek 4 + 450 counts @17:42:39.57 SE.reconfigure :pick @17:42:39.62 SE.seek :beamBreak @17:42:42.66 SE.configure :default @17:42:42.80 Elbow.seek 4 @17:42:42.96 Forearm.seek 4 + 300 counts @17:42:43.20 Forearm.reconfigure :loose @17:42:43.24 Hand.close @17:42:44.36 SE.seek :down @17:42:44.54
Forearm.seek :retract @17:42:45.48 Elbow.seek PC @17:42:47.14 SE.stop @17:42:48.00
Elbow.reconfigure :loose @17:42:48.09 Forearm.reconfigure :tight @17:42:48.14 Forearm.seek PC @17:42:49.34 PC.seek :closed @17:42:54.03 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.58 @17:42:54.04 Hand.open @17:42:54.65 Forearm.reconfigure :loose @17:42:54.69 Forearm.seek :retract @17:42:55.71 Heating Puck.from 4, :da in Processing Clamp to 28C for 2:00:00 @17:42:55.72 PH.seek 28C,5:00 @17:43:20.94 PH.hold 2:00:00,28C @17:43:20.98 delay 1:30 @17:44:51.02 PSR.select :air @17:44:51.03 PSV.dial PSR @17:44:52.11 Pulling 0.5ml of PSR.wash @17:44:52.12 PSR.select :wash @17:44:52.23 PS.seek 0.5ml @17:44:58.14 delay 5 seconds @17:45:03.15 PSR.select :air @17:45:03.27 PS.seek 2ml @17:45:19.41 PTV.dial :puck @17:45:20.13 PSV.dial PTV @17:45:20.88 PBV.dial :waste1 @17:45:22.26 PS.seek 1.5ml @17:45:28.16 delay 3 seconds @17:45:31.18 PBV.dial between PSV and waste1 @17:45:31.53 PS.seek 0ml @17:45:47.76 delay 5:00 @17:50:47.78 recoverPSRreagent08 PTV.dial :puck @17:50:47.79 PBV.dial :waste1 @17:50:48.14 delay 30 seconds @17:51:18.17 PBV.dial between PSV and waste1 @17:51:18.54 PSR.select :air @17:51:18.55 PSV.dial PSR @17:51:19.30 PS.reconfigure :fast @17:51:19.34 PS.seek 2.5ml @17:51:42.84 PSV.dial PTV @17:51:43.58 delay 5 seconds @17:51:48.60 PSV.dial PSR @17:51:49.34 delay 5 seconds @17:51:54.36 PSV.dial PTV @17:51:55.23 delay 5 seconds @17:52:00.25 PSV.dial :air @17:52:01.13 PS.seek 0ml @17:52:24.06 PTV.dial :PRVmixing,via: PRV @17:52:25.36 PSV.dial :mixloop @17:52:25.79 PRV.dial :ab1 @17:52:26.52 PS.reconfigure :slow @17:52:26.56 PS.seek 0.15ml @17:52:30.19 delay 2 seconds @17:52:32.20 CBV.dial PRV @17:52:32.23
PRV.dial CBV @17:52:32.78 PS.seek 0.35ml @17:52:37.37 delay 2 seconds @17:52:39.39 CBV.dial between PRV and PSV @17:52:39.69 PRV.dial :air @17:52:40.15 PS.seek 0.9ml @17:52:51.97 PRV.dial :waste2 @17:52:52.69 PS.seek 0.5ml @17:53:01.34 addPMLReagent08 @17:53:01.35 PSV.dial :air @17:53:01.82 PS.reconfigure :default @17:53:01.86 PS.seek 2ml @17:53:18.15 PSV.dial :PML @17:53:18.61 PTV.dial :mixing @17:53:19.34 PBV.dial :waste1 @17:53:20.23 PS.seek 1.85ml @17:53:22.56 PBV.dial between PSV and waste1 @17:53:22.93 PS.seek 0ml @17:53:42.81 delay 2 seconds @17:53:44.83 Beginning samplePassC @17:53:44.84 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @17:53:45.00 Elbow.reconfigure :tight @17:53:45.04 Elbow.seek FlushPuck::Garage @17:53:46.38 Elbow.reconfigure :loose @17:53:46.48 Forearm.seek :garage @17:53:47.41 Hand.close @17:53:48.07 Forearm.seek :retract @17:53:49.02 Elbow.reconfigure :tight @17:53:49.06 Elbow.seek CC @17:53:51.91 Elbow.reconfigure :loose @17:53:52.01 Forearm.reconfigure :tight @17:53:52.05 Forearm.seek CC @17:53:53.37 CC.seek :closed @17:53:57.90 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.37 @17:53:57.96 Hand.open @17:53:58.64 Forearm.reconfigure :loose @17:53:58.68 Forearm.seek :retract @17:53:59.62 PSV.dial PSR @17:54:00.83 Pulling 1.5ml of PSR.flush @17:54:00.84 PSR.select :flush @17:54:00.96 PS.seek 1.5ml @17:54:17.12 delay 5 seconds @17:54:22.14 PSV.dial :waste1 @17:54:22.61 PS.seek 0ml @17:54:38.81 PSV.dial PSR @17:54:39.26 PSR.select :air @17:54:39.37 PS.seek 2.5ml @17:55:05.72 PRV.dial CBV,avoiding: :ab2 @17:55:05.74
CBV.dial PRV @17:55:07.32 PSV.dial :mixloop @17:55:08.34 CTV.dial :air,avoiding: :intake @17:55:08.35 PTV.dial :PRVmixing @17:55:09.10 PS.seek 0ml @17:55:35.40 PSV.dial :air @17:55:35.85 PS.seek 2.5ml @17:56:02.12 PSV.dial :mixloop @17:56:02.58 PS.seek 0ml @17:56:28.96 PSV.dial :air @17:56:29.42 PS.seek 2.5ml @17:56:55.80 PSV.dial :mixloop @17:56:56.26 PS.seek 0ml @17:57:22.62 Garaging FlushPuck in Collection Clamp @17:57:22.69 Elbow.reconfigure :tight @17:57:22.73 Elbow.seek CC @17:57:23.04 Elbow.reconfigure :loose @17:57:23.12 Forearm.seek CC @17:57:24.14 Hand.close @17:57:24.70 CC.seek :open @17:57:29.31 Forearm.seek :retract @17:57:30.25 Elbow.reconfigure :tight @17:57:30.29 Elbow.seek :garage @17:57:33.18 Elbow.reconfigure :loose @17:57:33.26 Forearm.reconfigure :tight @17:57:33.31 Forearm.seek :garage @17:57:34.30 Hand.open @17:57:35.01 Forearm.reconfigure :loose @17:57:35.05 Forearm.seek :retract @17:57:36.02 delayUntil "18:01:44.82" @17:57:36.04 Whole Cell Archive sampling 1000ml @17:57:36.06 Loading wcr puck from tube 5 into Collection Clamp @17:57:36.28 SC.reconfigure :tight @17:57:36.32 SC.seek 5 @17:57:40.46 SC.configure :limp @17:57:40.66 Elbow.reconfigure :tight @17:57:40.70 Elbow.seek 5 @17:57:42.60 Forearm.reconfigure :tight @17:57:42.65 Forearm.seek 5 + 450 counts @17:57:43.62 SE.reconfigure :pick @17:57:43.67 SE.seek :beamBreak @17:57:44.57 SE.configure :default @17:57:44.72 Elbow.seek 5 @17:57:44.90 Forearm.seek 5 + 300 counts @17:57:45.12 Forearm.reconfigure :loose @17:57:45.17 Hand.close @17:57:45.84 SE.seek :down @17:57:46.03 Forearm.seek :retract @17:57:46.43 SE.stop @17:57:46.95 Elbow.seek CC @17:57:48.54 Elbow.reconfigure :loose @17:57:48.63 Forearm.reconfigure :tight @17:57:48.68 Forearm.seek CC @17:57:49.91 CC.seek :closed @17:57:54.42 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.24 @17:57:54.44 Hand.open @17:57:55.14 Forearm.reconfigure :loose @17:57:55.18 Forearm.seek :retract @17:57:56.21 collectSample 1000ml w/bubble point of 35psi @17:57:56.23 Priming 7ml Intake.select :close @17:57:56.24 Exhaust.select :close @17:57:56.25 SS.hold @17:57:56.45 Calibrating pressure sensors @17:57:56.46 CTV.dial :air @17:57:56.47 SSV.dial :spit,avoiding: :exhaust @17:57:56.49 SS.reconfigure :fast @17:57:56.53 SS.seek 5ml @17:57:59.24 SSV.dial CBV @17:57:59.26 CBV.dial SSV @17:58:00.60 delay 3.8 seconds @17:58:04.51 SSV.dial :spit @17:58:05.81 SS.seek 0ml @17:58:08.46 SSV.dial CBV @17:58:09.76 CTV.dial :intake @17:58:10.50 SSV.dial CBV @17:58:10.53 CBV.dial SSV @17:58:10.54 Allowing 40s for 7.0ml of remaining 7.0ml @17:58:10.55 Intake.select :open @17:58:12.71 Intake @15.1psia, vacuum from 9.6 to 12.6psi @17:58:12.73 SS.reconfigure :inhaling 2.20ml/s @17:58:12.78 SS.seek 7ml @17:58:19.66 Stroke completed -- waiting for pressure to equalize ... @17:58:19.67 SS.reconfigure :exhale @17:58:19.71 SS.hold @17:58:30.72 Intake.select :close @17:58:30.87 SSV.dial :exhaust @17:58:32.08 Exhaust.select :open @17:58:32.19 SS.seek 0ml @17:58:36.49 Exhaust.select :close @17:58:36.59 Sampling 1000.0ml @17:58:36.60 Intake.select :close @17:58:36.61 Exhaust.select :close @17:58:36.63 SS.reconfigure :default @17:58:36.67 SS.hold @17:58:36.86 CTV.dial :intake @17:58:36.88 SSV.dial CBV @17:58:36.91 CBV.dial SSV @17:58:37.66 Allowing 130s for 25.0ml of remaining 1000.0ml @17:58:37.68 Intake.select :open @17:58:39.83 Intake @16.3psia, vacuum from 10.0 to 13.0psi @17:58:39.85 SS.reconfigure :inhaling 2.20ml/s @17:58:39.89 SS.seek 25ml @17:58:45.17 Vacuum too high -- waiting up to 40 seconds @17:58:45.18 SS.reconfigure :exhale @17:58:45.22 SS.hold @17:58:49.70 SS.reconfigure :inhaling 1.98ml/s @17:58:49.74 SS.seek 25ml @17:58:53.01 Vacuum too high -- waiting up to 40 seconds @17:58:53.02 SS.reconfigure :exhale @17:58:53.06 SS.hold @17:58:58.98 Intake @16.2psia, vacuum from 10.0 to 13.0psi @17:58:59.00 SS.reconfigure :inhaling 1.79ml/s @17:58:59.04 SS.seek 25ml @17:59:02.54 Vacuum too high -- waiting up to 40 seconds @17:59:02.55 SS.reconfigure :exhale @17:59:02.60 SS.hold @17:59:10.25 Intake @16.0psia, vacuum from 10.0 to 13.0psi @17:59:10.26 SS.reconfigure :inhaling 1.61ml/s @17:59:10.31 SS.seek 25ml @17:59:13.76 Vacuum too high -- waiting up to 40 seconds @17:59:13.77 SS.reconfigure :exhale @17:59:13.82 SS.hold @17:59:22.47 Intake @15.8psia, vacuum from 10.0 to 13.0psi @17:59:22.49 SS.reconfigure :inhaling 1.45ml/s @17:59:22.53 SS.seek 25ml @17:59:26.66 Stroke completed -- waiting for pressure to equalize ... @17:59:26.67 SS.reconfigure :exhale @17:59:26.71 SS.hold @17:59:51.17 Intake.select :close @17:59:51.31 SSV.dial :exhaust @17:59:52.06 Exhaust.select :open @17:59:52.18 SS.seek 0ml @18:00:01.06 Exhaust.select :close @18:00:01.17 SSV.dial CBV @18:00:01.19 CBV.dial SSV @18:00:01.96 Allowing 130s for 25.0ml of remaining 975.0ml @18:00:01.97 Intake.select :open @18:00:04.12 Intake @15.7psia, vacuum from 10.0 to 13.0psi @18:00:04.14 SS.reconfigure :inhaling 1.45ml/s @18:00:04.18 SS.seek 25ml @18:00:11.48 Vacuum too high -- waiting up to 40 seconds @18:00:11.49 SS.reconfigure :exhale @18:00:11.53 SS.hold @18:00:27.91 Intake @16.0psia, vacuum from 10.0 to 13.0psi @18:00:27.93 SS.reconfigure :inhaling 1.30ml/s @18:00:27.97 SS.seek 25ml @18:00:30.98 Vacuum too high -- waiting up to 40 seconds @18:00:30.99 SS.reconfigure :exhale @18:00:31.03 SS.hold @18:00:47.35 SS.reconfigure :inhaling 1.17ml/s @18:00:47.39 SS.seek 25ml @18:00:50.95 Vacuum too high -- waiting up to 40 seconds @18:00:50.96 SS.reconfigure :exhale @18:00:51.00 SS.hold @18:01:15.44 Intake @15.9psia, vacuum from 10.0 to 13.0psi @18:01:15.46 SS.reconfigure :inhaling 1.06ml/s @18:01:15.50 SS.seek 25ml @18:01:19.41 Vacuum too high -- waiting up to 40 seconds @18:01:19.42 SS.reconfigure :exhale @18:01:19.47 SS.hold @18:01:44.82 recoverPRVreagent08 @18:01:44.83 PTV.dial :mixing @18:01:45.58 PBV.dial :waste1 @18:01:45.94 delay 30 seconds @18:01:50.57 Intake @16.0psia, vacuum from 10.0 to 13.0psi @18:01:50.59 SS.reconfigure :inhaling 0.95ml/s @18:01:50.63 SS.seek 25ml @18:01:54.98 Vacuum too high -- waiting up to 40 seconds @18:01:54.99 SS.reconfigure :exhale @18:01:55.03 SS.hold @18:02:11.98 SS.reconfigure :default @18:02:12.02 SS.hold @18:02:12.14 Intake.select :close @18:02:12.29 ShallowSampler::Clogged in WCR -- Filtering too Slowly after 44.7ml @18:02:12.30 SSV.dial :exhaust @18:02:13.05 Exhaust.select :open @18:02:13.15 SS.reconfigure :exhale @18:02:13.20 SS.seek 0ml @18:02:15.96 PBV.dial between PSV and waste1 @18:02:16.32 PSV.dial :air @18:02:16.79 PS.reconfigure :fast @18:02:16.83 PS.seek 2.5ml @18:02:21.03 Exhaust.select :close @18:02:21.14 Sampled 44.7ml @18:02:21.15 Drying puck before fixing for 20:00 @18:02:21.16 CBV.dial :waste2b @18:02:21.91 delay 1:00 @18:02:40.36 PSV.dial :mixloop @18:02:40.81 delay 2 seconds @18:02:42.83 PSV.dial :air @18:02:43.39 delay 2 seconds @18:02:45.41 PSV.dial :mixloop @18:02:45.86 delay 2 seconds @18:02:47.88 PSV.dial :air @18:02:48.34 PS.seek 0ml @18:03:11.36 washCycleDA #1 of 2 @18:03:11.37 PSR.select :air @18:03:11.39 PSV.dial PSR @18:03:12.73 Pulling 0.5ml of PSR.wash PSR.select :wash @18:03:12.84 PS.reconfigure :default @18:03:12.89 PS.seek 0.5ml @18:03:18.86 delay 5 seconds @18:03:21.92 CSV.dial :air @18:03:22.37 CS.reconfigure :fast @18:03:22.42 CS.seek 10ml @18:03:23.88 PSR.select :air @18:03:23.99 PS.seek 2ml @18:03:40.17 PTV.dial :puck @18:03:40.89 PSV.dial PTV @18:03:41.62 PBV.dial :waste1 @18:03:42.03 PS.seek 1.5ml @18:03:46.70 Pumping wcr puck from tube 5 up to 35psi... @18:03:46.71 CBV.dial SSV @18:03:47.89 delay 3 seconds @18:03:48.03 CBV.dial between CSV and waste2b @18:03:49.45 CSV.dial between air and CTV @18:03:49.79 CS.seek 7.321ml @18:03:50.91 PBV.dial between PSV and waste1 @18:03:51.27 PS.seek 0ml @18:03:56.61 CTV.dial CSV,avoiding: :air @18:03:56.63 CSV.dial CTV @18:03:57.45 CS.reconfigure :pressurize @18:03:57.50 CS.seek 0ml @18:04:07.49 delay 2:00 @18:04:15.90 CBV.dial :waste2b @18:04:16.22 CS.reconfigure :default @18:04:16.26 CS.seek 0ml @18:04:17.98 delay 1:00 @18:05:18.00 CTV.dial :air,avoiding: :intake @18:05:19.33 Fix #1 of 2 (with RNAlater) for 10:00 @18:05:19.34 CSV.dial CSR @18:05:19.83 Pulling 1.999ml of CSR.RNAlater @18:05:19.84 CSR.select :RNAlater @18:05:19.95 CS.seek 2ml @18:05:25.88 delay 5 seconds @18:05:30.90 CSR.select :air @18:05:31.02 CS.reconfigure :fast @18:05:31.07 CS.seek 10ml @18:05:50.57 Pumping wcr puck from tube 5 up to 35psi... @18:05:50.59 CBV.dial SSV @18:05:51.87 CBV.dial between CSV and waste2b @18:05:52.82 CSV.dial between air and CTV @18:05:53.53 CS.seek 7.267ml @18:06:00.51 CTV.dial CSV,avoiding: :air @18:06:00.54 CSV.dial CTV @18:06:01.87 CS.reconfigure :pressurize @18:06:01.92 CS.seek 0ml @18:06:07.51 recoverPSRreagent08 @18:06:07.52 PTV.dial :puck @18:06:07.53 PBV.dial :waste1 @18:06:07.87 delay 30 seconds @18:06:15.75 delay 10:00 @18:06:37.89 PBV.dial between PSV and waste1 @18:06:38.26 PSR.select :air @18:06:38.28 PSV.dial PSR @18:06:39.03 PS.reconfigure :fast @18:06:39.08 PS.seek 2.5ml @18:07:02.70 PSV.dial PTV @18:07:03.44 delay 5 seconds @18:07:08.46 PSV.dial PSR @18:07:09.20 delay 5 seconds @18:07:14.22 PSV.dial PTV @18:07:14.97 delay 5 seconds @18:07:19.99 PSV.dial :air @18:07:20.74 PS.seek 0ml @18:07:43.64 washCycleDA #2 of 2 @18:07:43.65 PSR.select :air @18:07:43.66 PSV.dial PSR @18:07:45.00 Pulling 0.5ml of PSR.wash @18:07:45.01 PSR.select :wash @18:07:45.12 PS.reconfigure :default @18:07:45.17 PS.seek 0.5ml @18:07:51.14 delay 5 seconds @18:07:56.16 PSR.select :air @18:07:56.27 PS.seek 2ml @18:08:12.41 PTV.dial :puck @18:08:12.43 PSV.dial PTV @18:08:13.28 PBV.dial :waste1 @18:08:13.69 PS.seek 1.5ml @18:08:19.60 delay 3 seconds @18:08:22.62 PBV.dial between PSV and waste1 @18:08:23.00 PS.seek 0ml @18:08:39.20 delay 2:00 @18:10:39.22 recoverPSRreagent08 @18:10:39.23 PTV.dial :puck @18:10:39.24 PBV.dial :waste1 @18:10:39.60 delay 30 seconds @18:11:09.63 PBV.dial between PSV and waste1 @18:11:09.99 PSR.select :air @18:11:10.01 PSV.dial PSR @18:11:10.87 PS.reconfigure :fast @18:11:10.91 PS.seek 2.5ml @18:11:34.38 PSV.dial PTV @18:11:35.12 delay 5 seconds @18:11:40.14 PSV.dial PSR @18:11:40.88 delay 5 seconds @18:11:45.90 PSV.dial PTV @18:11:46.65 delay 5 seconds @18:11:51.67 PSV.dial :air @18:11:52.42 PS.seek 0ml @18:12:15.29 getPMLReagent08 @18:12:15.30 PRV.dial :air @18:12:15.74 delay 5 seconds @18:12:20.76 PTV.dial :PRVmixing @18:12:22.54 delay 5 seconds @18:12:27.56 PRV.dial :ab2 @18:12:28.01 PTV.dial :PRVmixing @18:12:28.02 PSV.dial :mixloop @18:12:28.48 PS.reconfigure :default @18:12:28.52 PS.seek 0.3ml @18:12:32.38 delay 2 seconds @18:12:34.40 PRV.dial :air @18:12:34.89 PS.seek 0.55ml @18:12:38.19 addPMLReagent08 @18:12:38.20 PSV.dial :air @18:12:38.78 PS.seek 2ml @18:12:54.41 PSV.dial :PML @18:12:54.85 PTV.dial :mixing @18:12:55.60 PBV.dial :waste1 @18:12:56.03 PS.seek 1.85ml @18:12:58.41 PBV.dial between PSV and waste1 @18:12:58.78 PS.seek 0ml @18:13:18.47 delay 2 seconds @18:13:20.49 delay 8:00 @18:16:15.77 CBV.dial :waste2b @18:16:16.11 CS.reconfigure :default @18:16:16.16 CS.seek 0ml @18:16:22.28 delay 1:00 @18:17:22.31 CTV.dial :air,avoiding: :intake @18:17:23.61 Fix #2 of 2 (with RNAlater) for 10:00 @18:17:23.62 CSV.dial CSR @18:17:24.22 Pulling 2ml of CSR.RNAlater @18:17:24.23 CSR.select :RNAlater @18:17:24.34 CS.seek 2ml @18:17:30.28 delay 5 seconds @18:17:35.30 CSR.select :air @18:17:35.41 CS.reconfigure :fast @18:17:35.45 CS.seek 10ml @18:17:54.97 Pumping wcr puck from tube 5 up to 35psi... @18:17:54.98 CBV.dial SSV @18:17:56.27 CBV.dial between CSV and waste2b @18:17:57.21 CSV.dial between air and CTV @18:17:57.91 CS.seek 7.184ml @18:18:05.07 CTV.dial CSV,avoiding: :air @18:18:05.08 CSV.dial CTV @18:18:06.47 CS.reconfigure :pressurize @18:18:06.52 CS.seek 0ml @18:18:21.20 delay 10:00 @18:21:20.52 recoverPRVreagent08 @18:21:20.53 PTV.dial :mixing @18:21:20.54 PBV.dial :waste1 @18:21:20.89 delay 30 seconds @18:21:50.91 PBV.dial between PSV and waste1 @18:21:51.26 PSV.dial :air @18:21:51.73 PS.reconfigure :fast @18:21:51.78 PS.seek 2.5ml @18:22:15.21 PSV.dial :mixloop @18:22:15.67 delay 2 seconds @18:22:17.68 PSV.dial :air @18:22:19.12 delay 2 seconds @18:22:21.14 PSV.dial :mixloop @18:22:21.59 delay 2 seconds @18:22:23.61 PSV.dial :air @18:22:24.08 PS.seek 0ml @18:22:47.21 washCycleDA #1 of 3 @18:22:47.22 PSR.select :air @18:22:47.24 PSV.dial PSR @18:22:48.58 Pulling 0.5ml of PSR.wash @18:22:48.59 PSR.select :wash @18:22:48.70 PS.reconfigure :default @18:22:48.74 PS.seek 0.5ml @18:22:54.78 delay 5 seconds @18:22:59.79 PSR.select :air @18:22:59.91 PS.seek 2ml @18:23:16.08 PTV.dial :puck @18:23:16.80 PSV.dial PTV @18:23:17.54 PBV.dial :waste1 @18:23:17.94 PS.seek 1.5ml @18:23:23.87 delay 3 seconds @18:23:26.89 PBV.dial between PSV and waste1 @18:23:27.25 PS.seek 0ml @18:23:43.34 delay 2:00 @18:25:43.36 recoverPSRreagent08 @18:25:43.37 PTV.dial :puck @18:25:43.38 PBV.dial :waste1 @18:25:43.73 delay 30 seconds @18:26:13.75 PBV.dial between PSV and waste1 @18:26:14.11 PSR.select :air @18:26:14.13 PSV.dial PSR @18:26:14.88 PS.reconfigure :fast @18:26:14.93 PS.seek 2.5ml @18:26:38.46 PSV.dial PTV @18:26:39.19 delay 5 seconds @18:26:44.21 PSV.dial PSR @18:26:44.96 delay 5 seconds @18:26:49.98 PSV.dial PTV @18:26:50.72 delay 5 seconds @18:26:55.74 PSV.dial :air @18:26:56.97 PS.seek 0ml @18:27:19.93 washCycleDA #2 of 3 @18:27:19.94 PSR.select :air @18:27:19.95 PSV.dial PSR @18:27:21.29 Pulling 0.5ml of PSR.wash @18:27:21.30 PSR.select :wash @18:27:21.41 PS.reconfigure :default @18:27:21.46 PS.seek 0.5ml @18:27:27.37 delay 5 seconds @18:27:32.38 PSR.select :air @18:27:32.50 PS.seek 2ml @18:27:48.64 PTV.dial :puck @18:27:48.66 PSV.dial PTV @18:27:49.38 PBV.dial :waste1 @18:27:49.81 PS.seek 1.5ml @18:27:55.67 delay 3 seconds @18:27:58.69 PBV.dial between PSV and waste1 @18:27:59.06 PS.seek 0ml @18:28:15.24 delay 2:00 @18:28:21.23 CBV.dial :waste2b @18:28:21.55 CS.reconfigure :default @18:28:21.60 CS.seek 0ml @18:28:26.58 delay 1:00 @18:29:26.61 CTV.dial :air,avoiding: :intake @18:30:15.26 recoverPSRreagent08 @18:30:15.27 PTV.dial :puck @18:30:15.28 PBV.dial :waste1 @18:30:15.64 delay 30 seconds @18:30:45.67 PBV.dial between PSV and waste1 @18:30:46.02 PSR.select :air @18:30:46.03 PSV.dial PSR @18:30:46.79 PS.reconfigure :fast @18:30:46.83 PS.seek 2.5ml @18:31:10.36 PSV.dial PTV @18:31:11.11 delay 5 seconds @18:31:16.13 PSV.dial PSR @18:31:16.87 delay 5 seconds @18:31:21.89 PSV.dial PTV @18:31:22.64 delay 5 seconds @18:31:27.66 PSV.dial :air @18:31:28.41 PS.seek 0ml @18:31:51.39 washCycleDA #3 of 3 @18:31:51.40 PSR.select :air @18:31:51.42 PSV.dial PSR @18:31:52.75 Pulling 0.5ml of PSR.wash @18:31:52.76 PSR.select :wash @18:31:52.87 PS.reconfigure :default @18:31:52.92 PS.seek 0.5ml @18:31:58.89 delay 5 seconds @18:32:03.91 PSR.select :air @18:32:04.02 PS.seek 2ml @18:32:20.23 PTV.dial :puck @18:32:20.24 PSV.dial PTV @18:32:20.96 PBV.dial :waste1 @18:32:21.37 PS.seek 1.5ml @18:32:27.26 delay 3 seconds @18:32:30.28 PBV.dial between PSV and waste1 @18:32:30.65 PS.seek 0ml @18:32:46.83 delay 2:00 @18:34:46.84 recoverPSRreagent08 @18:34:46.85 PTV.dial :puck @18:34:46.86 PBV.dial :waste1 @18:34:47.21 delay 30 seconds @18:35:17.24 PBV.dial between PSV and waste1 @18:35:17.59 PSR.select :air @18:35:17.61 PSV.dial PSR @18:35:18.36 PS.reconfigure :fast @18:35:18.40 PS.seek 2.5ml @18:35:42.01 PSV.dial PTV @18:35:42.74 delay 5 seconds @18:35:47.76 PSV.dial PSR @18:35:48.51 delay 5 seconds @18:35:53.53 PSV.dial PTV @18:35:54.27 delay 5 seconds @18:35:59.29 PSV.dial :air @18:36:00.15 PS.seek 0ml @18:36:23.29 Gate.power :camera,:ON @18:36:23.30 Mixing DA Substrates @18:36:23.31 PSR.select :air @18:36:23.33 PSV.dial PSR @18:36:24.95 Pulling 0.25ml of PSR.sub1 @18:36:24.98 PSR.select :sub1 @18:36:25.18 PS.reconfigure :default @18:36:25.28 PS.seek 0.25ml @18:36:28.70 delay 5 seconds @18:36:33.76 Pulling 0.25ml of PSR.sub2 @18:36:33.77 PSR.select :sub2 @18:36:33.89 PS.seek 0.5ml @18:36:37.48 delay 5 seconds @18:36:42.50 PSR.select :air @18:36:42.60 PS.seek 2ml @18:36:58.72 PTV.dial :puck @18:36:58.74 PSV.dial PTV @18:36:59.48 PBV.dial :waste1 @18:36:59.91 PS.seek 1.5ml @18:37:05.91 delay 3 seconds @18:37:08.93 PBV.dial between PSV and waste1 @18:37:09.31 PS.seek 0ml @18:37:25.41 delay 10 seconds @18:37:35.44 recoverPSRreagent08 @18:37:35.45 PTV.dial :puck @18:37:35.46 PBV.dial :waste1 @18:37:35.82 delay 30 seconds @18:38:05.85 PBV.dial between PSV and waste1 @18:38:06.21 PSR.select :air @18:38:06.23 PSV.dial PSR @18:38:07.00 PS.reconfigure :fast @18:38:07.04 PS.seek 2.5ml @18:38:31.00 PSV.dial PTV @18:38:31.76 delay 5 seconds @18:38:36.78 PSV.dial PSR @18:38:37.54 delay 5 seconds @18:38:43.05 PSV.dial PTV @18:38:43.79 delay 5 seconds @18:38:48.81 PSV.dial :air @18:38:49.69 PS.seek 0ml @18:39:13.10 Additional air clear @18:39:13.11 PSR.select :air @18:39:13.13 PSV.dial PSR @18:39:14.49 Pulling 2ml of PSR.air @18:39:14.50 PSR.select :air @18:39:14.53 PS.reconfigure :default @18:39:14.57 PS.seek 2ml @18:39:35.88 delay 5 seconds @18:39:40.90 PTV.dial :puck @18:39:40.92 PSV.dial PTV @18:39:41.68 PBV.dial :waste1 @18:39:42.11 PS.seek 1.5ml @18:39:48.00 delay 3 seconds @18:39:51.02 PBV.dial between PSV and waste1 @18:39:51.40 delay 1 second @18:39:52.41 PSV.dial :waste1 @18:39:53.59 PS.seek 0ml @18:40:09.71 Processing Puck Heater at 27.9C before imaging @18:40:09.72 PH.coast @18:40:09.84 Imaging da puck in Processing Clamp while storing it to tube 3 @18:40:09.99 SC.reconfigure :tight @18:40:10.04 SC.seek 3 @18:40:16.78 SC.configure :limp @18:40:16.97 Elbow.reconfigure :tight @18:40:17.02 Elbow.seek 3 @18:40:18.80 Forearm.reconfigure :tight @18:40:18.85 Forearm.seek 3 + 450 counts @18:40:19.80 SE.reconfigure :pick @18:40:19.85 SE.seek :beamBreak @18:40:20.94 SE.configure :default @18:40:21.08 Elbow.reconfigure :limp @18:40:21.12 Forearm.reconfigure :loose @18:40:21.17 Forearm.seek 3 @18:40:21.45 Elbow.hold @18:40:21.51 Elbow.reconfigure :tight @18:40:21.60 SE.seek 31.09 Pucks below Top Plate @18:40:22.09 Forearm.force -20 @18:40:22.13 Forearm.seek 3 + 295 counts @18:40:22.40 Forearm.reconfigure :tight @18:40:22.44 Forearm.seek 3 + 450 counts @18:40:22.66 SE.seek 32.09 Pucks below Top Plate @18:40:23.21 Forearm.reconfigure :loose @18:40:23.25 Forearm.seek :retract @18:40:24.19 Elbow.seek PC @18:40:26.66 Elbow.reconfigure :loose @18:40:26.74 Forearm.seek PC @18:40:27.74 Hand.close @18:40:28.29 PC.seek :open @18:40:33.04 Forearm.seek :retract @18:40:33.98 Elbow.reconfigure :tight @18:40:34.03 Elbow.seek 3 @18:40:36.63 Forearm.reconfigure :tight @18:40:36.68 Forearm.seek 3 + 450 counts @18:40:37.62 Hand.open @18:40:38.23 SE.seek 31.74 Pucks below Top Plate @18:40:38.47 SE.reconfigure :seal @18:40:38.52 SE.seek 30.78 Pucks below Top Plate @18:40:39.07 Lights, camera, ACTION! @18:40:39.10 PuckCamera: snap -bin=2,2 -auto /var/log/esp/da23mar2718h75ml.tif @18:40:58.57 Waiting >20 seconds for da to relinquish FlushPuck @18:42:25.62 SX HX9/16 (USB:25): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 103.034 seconds /var/log/esp/da23mar2718h75ml.tif: TIFF Upload Complete @18:42:25.65 PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da23mar2718h75ml15s.tif @18:42:41.26 SX HX9/16 (USB:25): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15 seconds /var/log/esp/midres/da23mar2718h75ml15s.tif: TIFF Upload Complete @18:42:41.29 PuckCamera: snap -bin=2,2 20.0 /var/log/esp/midres/da23mar2718h75ml20s.tif @18:43:01.94 SX HX9/16 (USB:25): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 20 seconds /var/log/esp/midres/da23mar2718h75ml20s.tif: TIFF Upload Complete @18:43:01.96 Gate.power :camera,:OFF @18:43:01.98 DAfans.email "103.034 sec auto-exposure",Subject: "da23mar2718h75ml images!" @18:43:02.28 Elbow.seek 3 @18:43:02.53 Forearm.seek 3 + 450 counts @18:43:02.60 SE.reconfigure :gently @18:43:02.66 SE.seek 31.49 Pucks below Top Plate @18:43:03.32 Elbow.reconfigure :limp @18:43:03.97 Forearm.reconfigure :loose @18:43:04.01 Forearm.seek 3 @18:43:04.31 Elbow.hold @18:43:04.39 Elbow.reconfigure :tight @18:43:04.44 SE.reconfigure :default @18:43:04.49 SE.seek 31.68 Pucks below Top Plate @18:43:04.97 Forearm.force -20 @18:43:05.00 Forearm.seek 3 + 284 counts @18:43:05.28 Forearm.reconfigure :tight @18:43:05.33 Forearm.seek 3 + 450 counts @18:43:05.87 SE.seek :down @18:43:06.52 SE.stop @18:43:07.12 Forearm.reconfigure :loose @18:43:07.21 Forearm.seek :retract @18:43:08.29 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @18:43:08.80 Elbow.seek FlushPuck::Garage @18:43:10.76 Elbow.reconfigure :loose @18:43:10.95 Forearm.seek :garage @18:43:11.94 Hand.close @18:43:12.83 Forearm.seek :retract @18:43:13.85 Elbow.reconfigure :tight @18:43:13.94 Elbow.seek PC @18:43:15.49 Elbow.reconfigure :loose @18:43:15.68 Forearm.reconfigure :tight @18:43:15.76 Forearm.seek PC @18:43:17.33 PC.seek :closed @18:43:22.22 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.60 @18:43:22.40 Hand.open @18:43:23.18 Forearm.reconfigure :loose @18:43:23.26 Forearm.seek :retract @18:43:24.28 PSR.select :air @18:43:24.34 Storing wcr puck in Collection Clamp to tube 4 @18:43:24.37 PSV.dial PSR @18:43:24.78 SC.reconfigure :tight @18:43:24.87 SC.seek 4 @18:43:25.10 Pulling 0.5ml of PSR.flush @18:43:25.12 PSR.select :flush @18:43:25.30 PS.seek 0.5ml @18:43:28.52 SC.configure :limp @18:43:28.89 SE.reconfigure :place @18:43:29.08 Elbow.reconfigure :tight @18:43:29.17 Elbow.seek CC @18:43:29.26 SE.seek :beamBreak @18:43:31.25 delay 5 seconds @18:43:32.18 SE.configure :default @18:43:32.28 SE.seek :place @18:43:32.94 Elbow.reconfigure :loose @18:43:33.14 Forearm.seek CC @18:43:34.32 Hand.close @18:43:34.95 CC.seek :open @18:43:36.28 PSR.select :air @18:43:36.43 PS.seek 2.5ml @18:43:39.99 Forearm.seek :retract @18:43:41.07 Elbow.reconfigure :tight @18:43:41.16 Elbow.seek 4 @18:43:44.15 Elbow.reconfigure :limp @18:43:44.28 Forearm.seek 4 @18:43:45.34 Elbow.hold @18:43:45.48 Elbow.reconfigure :tight @18:43:45.57 Hand.open @18:43:46.44 Forearm.force -20 @18:43:46.52 Forearm.seek 4 + 291 counts @18:43:46.99 Forearm.reconfigure :tight @18:43:47.07 Forearm.seek 4 + 450 counts @18:43:47.48 SE.seek :down @18:43:48.66 Forearm.reconfigure :loose @18:43:48.72 Forearm.seek :retract @18:43:49.13 upsync @18:43:50.22 SE.stop @18:43:50.25
Starting finalPurge @18:43:57.75 delay 1 second @18:43:58.77 PRV.dial :waste2,avoiding: :ab2 @18:44:00.59 PTV.dial :PRVmixing @18:44:01.89 PSV.dial :mixloop @18:44:03.03 PS.seek :empty @18:44:10.10
Waiting >20 seconds for psFlush to relinquish FlushPuck @18:44:29.40 PSR.select :air @18:44:29.42 PSV.dial PSR @18:44:30.47 Pulling 0.5ml of PSR.flush @18:44:30.48 PSR.select :flush @18:44:30.60 PS.seek 0.5ml @18:44:36.72 delay 5 seconds @18:44:41.74 PSR.select :air @18:44:41.85 PS.seek 2.5ml @18:45:03.03 delay 1 second @18:45:04.05 PBV.dial :waste1 @18:45:04.40 PTV.dial :mixing @18:45:05.14 PSV.dial :mixloop @18:45:06.17 PS.seek :empty @18:45:32.50 PSV.dial PSR @18:45:33.53 PSR.select :flush @18:45:33.64 PS.seek 1ml @18:45:44.77 PSR.select :air @18:45:44.89 PS.seek 2.5ml @18:46:01.09 PSV.dial PTV @18:46:01.82 PTV.dial :puck @18:46:02.56 PBV.dial :waste1 @18:46:02.58 PS.seek 0ml @18:46:29.12 PSV.dial PSR @18:46:29.85 PSR.select :flush @18:46:29.97 PS.seek 0.5ml @18:46:35.84 PSR.select :air @18:46:35.95 PS.seek 2.5ml @18:46:57.21 PSV.dial PTV @18:46:57.96 PBV.dial PSV @18:46:58.52 PS.seek 0ml @18:47:24.90 PSV.dial PBV @18:47:25.36 PS.seek 2.5ml @18:47:51.71 PSV.dial :waste1 @18:47:52.45 PS.seek 0ml @18:48:18.77 PSV.dial PSR @18:48:19.23 PSR.select :air @18:48:19.25 PS.seek 2.5ml @18:48:45.62 PSV.dial PTV @18:48:46.34 PBV.dial PSV @18:48:46.36 PS.seek 0ml @18:49:12.71 PSV.dial PBV @18:49:13.19 PS.seek 2.5ml @18:49:39.52 PSV.dial :waste1 @18:49:40.26 PS.seek 0ml @18:50:06.61 PSV.dial PSR @18:50:07.07 PSR.select :flush @18:50:07.18 PS.seek 1ml @18:50:18.27 PSR.select :air @18:50:18.38 PS.seek 2.5ml @18:50:34.55 PSV.dial PTV @18:50:35.27 PTV.dial :puck @18:50:35.28 PBV.dial :waste1 @18:50:35.84 PS.seek 0ml @18:51:02.24 PSV.dial PSR @18:51:02.97 PSR.select :flush @18:51:03.09 PS.seek 0.5ml @18:51:09.05 PSR.select :air @18:51:09.16 PS.seek 2.5ml @18:51:30.46 PSV.dial PTV @18:51:31.68 PBV.dial PSV @18:51:32.26 PS.seek 0ml @18:51:58.62 PSV.dial PBV @18:51:59.14 PS.seek 2.5ml @18:52:25.46 PSV.dial :waste1 @18:52:26.18 PS.seek 0ml @18:52:52.52 PSV.dial PSR @18:52:53.10 PSR.select :air @18:52:53.12 PS.seek 2.5ml @18:53:19.49 PSV.dial PTV @18:53:20.24 PBV.dial PSV @18:53:20.26 PS.seek 0ml @18:53:46.61 PSV.dial PBV @18:53:47.09 PS.seek 2.5ml @18:54:13.45 PSV.dial :waste1 @18:54:14.18 PS.seek 0ml @18:54:40.52
Loading FlushPuck into Collection Clamp from Processing Clamp @18:54:40.69 Elbow.seek PC @18:54:43.33 Elbow.reconfigure :loose @18:54:43.42 Forearm.seek PC @18:54:44.40 Hand.close @18:54:45.03 PC.seek :open @18:54:49.75 Forearm.seek :retract @18:54:50.70 Elbow.reconfigure :tight @18:54:50.75 Elbow.seek CC @18:54:54.32 Elbow.reconfigure :loose @18:54:54.40 Forearm.reconfigure :tight @18:54:54.44 Forearm.seek CC @18:54:55.66 CC.seek :closed @18:55:00.17 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.37 @18:55:00.24 Hand.open @18:55:00.96 Forearm.reconfigure :loose @18:55:01.00 Forearm.seek :retract @18:55:02.00 Flushing the Collection Valve::Manifold @18:55:02.12 CSV.dial CSR @18:55:02.71 Pulling 5ml of CSR.flush @18:55:02.72 CSR.select :flush @18:55:02.83 CS.seek 5ml @18:55:16.40 delay 5 seconds @18:55:21.41 CSR.select :air @18:55:21.56 CS.reconfigure :fast @18:55:21.60 CS.seek 7.5ml @18:55:28.24 CSV.dial :waste @18:55:29.48 CS.reconfigure :default @18:55:29.52 CS.seek 0ml @18:55:49.52 Flushing lower manifold's waste1 port @18:55:49.64 CSV.dial :flush @18:55:50.09 CS.reconfigure :fast @18:55:50.13 CS.seek 5ml @18:56:02.74 delay 5 seconds @18:56:07.75 CSV.dial :air @18:56:08.25 CS.seek 7ml @18:56:13.74 CSV.dial CTV @18:56:13.76
CTV.dial CSV @18:56:15.11 CBV.dial :waste1 @18:56:15.85 CS.seek 0ml @18:56:32.52 Flushing lower manifold's waste2 port @18:56:32.63 CSV.dial :flush @18:56:33.37 CS.seek 5ml @18:56:46.33 delay 5 seconds @18:56:51.35 CSV.dial :air @18:56:51.86 CS.seek 7ml @18:56:57.36 CSV.dial CTV @18:56:57.38
CTV.dial CSV @18:56:57.83 CBV.dial :waste2 @18:56:58.85 CS.seek 0ml @18:57:15.46 Flushing lower manifold's waste2b port @18:57:15.57 CSV.dial :flush @18:57:16.41 CS.seek 5ml @18:57:29.14 delay 5 seconds @18:57:34.16 CSV.dial :air @18:57:34.65 CS.seek 7ml @18:57:40.08 CSV.dial CTV @18:57:40.10
CTV.dial CSV @18:57:40.55 CBV.dial :waste2b @18:57:42.06 CS.seek 0ml @18:57:58.45 Disinfecting external Intake with kill @18:57:58.57 CSV.dial CSR @18:57:59.06 Pulling 8ml of CSR.kill CSR.select :kill @18:57:59.17 CS.seek 8ml @18:58:18.80 delay 5 seconds @18:58:23.82 CSR.select :air @18:58:23.93 CS.seek 10ml @18:58:29.37 CSV.dial CBV @18:58:29.41
CBV.dial CSV @18:58:30.39 CTV.dial :intake @18:58:31.15 Intake.select :open @18:58:31.26 CS.seek 2ml @18:58:49.89 Intake.select :close @18:58:49.99 CTV.dial :air @18:58:50.74 CS.reconfigure :default @18:58:50.78 CS.seek 0ml @18:58:56.92 CSV.dial CSR @18:58:57.96 Pulling 5ml of CSR.flush @18:58:57.97 CSR.select :flush @18:58:58.08 CS.seek 5ml @18:59:11.64 delay 5 seconds @18:59:16.66 CSR.select :air @18:59:16.77 CS.seek 10ml @18:59:30.29 CSV.dial CBV @18:59:31.29 CS.seek 0ml @18:59:57.86 Garaging FlushPuck in Collection Clamp @18:59:57.93 Elbow.reconfigure :tight @18:59:57.97 Elbow.seek CC @18:59:58.23 Elbow.reconfigure :loose @18:59:58.32 Forearm.seek CC @18:59:59.27 Hand.close @18:59:59.90 CC.seek :open @19:00:04.50 Forearm.seek :retract @19:00:05.46 Elbow.reconfigure :tight @19:00:05.51 Elbow.seek :garage @19:00:08.39 Elbow.reconfigure :loose @19:00:08.48 Forearm.reconfigure :tight @19:00:08.53 Forearm.seek :garage @19:00:09.55 Hand.open @19:00:10.21 Forearm.reconfigure :loose @19:00:10.25 Forearm.seek :retract @19:00:11.21 Completed [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] begun 17:05:15 @19:00:11.26 Asleep after completing DA_WCR_032723 assays @19:00:11.28 GoodNews.email "{1 => 33,\n 2 => 33,\n 3 => 33,\n 4 => 1,\n 5 => 32,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@18:54:26, 16% flow, 23.5C, 44% humidity, 15.0psia, 13.769V, 0.339A, 0.315A avg, 4.66W\nSampled 44.7ml",Subject: "Asleep after completing DA_WCR_032723 assays" @19:00:16.14 upsync @20:56:23.23UTC15-Apr-23
ESP2 v3.41 I2C Gateway v3.20 for core bus @20:56:25.83 Configuration for short puck jake 1/11/21 by brent@mbari.org /home/esp/esp2/type/shallow/jake/configure.rb @20:56:29.70 Polling Can every 10:00 @20:56:29.99 Cleanly exited at 20:52:30UTC15-Apr-23 @20:56:30.02 Loading /home/esp/esp2/mission/DA_WCR_041523.rb mission @20:56:30.07 Preparing to sleep until + 00:00:05 @20:56:30.09 GoodNews.email "Starting DA_WCR_041523 w/pucks in tube 5\n{1 => 33,\n 2 => 33,\n 3 => 33,\n 4 => 1,\n 5 => 32,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@20:56:29, 16% flow, 21.5C, 52% humidity, 15.0psia, 13.632V, 0.302A, 0.290A avg, 4.12W",Subject: "DA_WCR_041523 begun" @20:56:30.14
Asleep til + 00:00:05 @20:56:30.17 Continuing at 20:56 @20:56:30.42 Gate.power :core,:OFF @20:56:30.43 Gate.power :raw,:OFF @20:56:30.44 Shutdown @20:56:32.87 GoodNews.email "Then, in tube 5: initialPurge; da [100, 100] #da and wsr are run simultanelously\n/home/esp/esp2/mission/DA_WCR_041523.rb:14\n{1 => 33,\n 2 => 33,\n 3 => 33,\n 4 => 1,\n 5 => 32,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@20:56:30, 16% flow, 21.5C, 52% humidity, 15.0psia, 13.632V, 0.302A, 0.290A avg, 4.12W",Subject: "Asleep until 20:56" @20:56:34.45 upsync @20:56:35.05
Powering core bus @20:56:36.82 upsync @20:56:40.77
Gate.power :raw,:ON @20:56:41.40 CS.configure :default @20:56:41.49 PS.configure :default @20:56:41.58 SS.configure :default @20:56:41.67 CC.configure :default @20:56:41.76 PC.configure :default @20:56:41.87 Forearm.configure :loose @20:56:41.96 Elbow.configure :loose @20:56:42.06 SC.configure :loose @20:56:42.15 SE.configure :default @20:56:42.19 CH.coast @20:56:42.24 CH.configure :default @20:56:42.27 PH.coast @20:56:42.33 PH.configure :default @20:56:42.36 All dwarf controllers running firmware version 3.85 @20:56:42.48 Valve.reset @20:56:42.72 Intake.select :close @20:56:42.76 Exhaust.select :close @20:56:42.77 CTV.dial :air @20:56:42.86 PSV.dial :air @20:56:42.97 CSV.dial :waste1 @20:56:43.00
Forearm.reconfigure :loose @20:56:43.08 Forearm.home.seek :retract @20:56:43.12 SSV.dial :spit,avoiding: :exhaust @20:56:43.13 PS.reconfigure :default @20:56:43.28 PS.home.seek 0ml @20:56:43.35 CS.reconfigure :fast @20:56:43.38 SS.reconfigure :default @20:56:43.46 CS.home.seek 0ml @20:56:43.49 SS.home.seek 0ml @20:56:43.88 Hand.close @20:56:43.93
Elbow.jog 1500 @20:56:44.43 Elbow.reconfigure :loose @20:56:44.49 Elbow.home.seek :clear @20:56:49.00 SE.reconfigure :default @20:56:49.04 SE.home.seek :down @20:56:49.64 SC.reconfigure :tight @20:56:49.68 SC.home.seek :initial @20:56:51.55 PS.stop @20:57:18.07
SC.configure :limp @20:57:18.12 Forearm.seek :retract @20:57:18.18 Elbow.seek :hammer @20:57:19.19 Forearm.reconfigure :tight @20:57:19.23 Forearm.seek retracted @20:57:19.46 PC.reconfigure :default @20:57:19.49
CC.reconfigure :default @20:57:19.56 PC.home.seek :open @20:57:19.62
CC.home.seek :open @20:57:23.08 Phase [initialPurge; da [100, 100] #da and wsr are run simultanelously] @20:57:23.13 Notice.email "At 20:57:22 began in tube 5: initialPurge; da [100, 100] #da and wsr are run simultanelously\n/home/esp/esp2/mission/DA_WCR_041523.rb:14\n{1 => 33,\n 2 => 33,\n 3 => 33,\n 4 => 1,\n 5 => 32,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@20:57:23, 16% flow, 21.4C, 52% humidity, 15.0psia, 13.598V, 0.302A, 0.290A avg, 4.11W",Subject: "Started DA_WCR_041523 phase initialPurge; da [100, 100] #da and wsr are run simultanelously" @20:57:23.15
Starting initialPurge @20:57:23.18 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @20:57:23.25 Hand.open @20:57:23.99 Elbow.reconfigure :tight @20:57:24.03 Elbow.seek FlushPuck::Garage @20:57:25.92 Elbow.reconfigure :loose @20:57:26.11 Forearm.reconfigure :loose @20:57:26.21 Forearm.seek :garage @20:57:27.20 Hand.close @20:57:27.43 upsync @20:57:27.98
Forearm.seek :retract @20:57:28.97 Elbow.reconfigure :tight @20:57:29.02 Elbow.seek CC @20:57:31.93 Elbow.reconfigure :loose @20:57:32.03 Forearm.reconfigure :tight @20:57:32.07 Forearm.seek CC @20:57:33.28 CC.seek :closed @20:57:37.81 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.35 @20:57:37.87 Hand.open @20:57:38.55 Forearm.reconfigure :loose @20:57:38.60 Forearm.seek :retract @20:57:39.57 Flushing the Collection Valve::Manifold @20:57:39.69 CSV.dial CSR @20:57:41.04 Pulling 5ml of CSR.flush CSR.select :flush @20:57:41.16 CS.reconfigure :default @20:57:41.20 CS.seek 5ml @20:57:55.00 delay 5 seconds @20:58:00.02 CSR.select :air @20:58:00.17 CS.reconfigure :fast @20:58:00.21 CS.seek 7.5ml @20:58:06.96 CSV.dial :waste @20:58:08.36 CS.reconfigure :default @20:58:08.40 CS.seek 0ml @20:58:28.34 Purging external Intake @20:58:28.46 CSV.dial :flush @20:58:28.92 CS.reconfigure :fast @20:58:28.97 CS.seek 10ml @20:58:53.53 delay 5 seconds @20:58:58.55 CSV.dial CBV @20:58:58.58
CBV.dial CSV @20:58:59.34 CTV.dial :intake @20:59:00.15 Intake.select :open @20:59:00.26 CS.seek 0ml @20:59:23.90 Intake.select :close @20:59:24.00 CTV.dial :air @20:59:24.81 Flushing upper manifold @20:59:24.94 CSV.dial :flush @20:59:25.80 CS.seek 5ml @20:59:38.56 delay 5 seconds @20:59:43.58 CSV.dial :air @20:59:44.10 CS.seek 7ml @20:59:49.81 CSV.dial CBV @20:59:49.83
CBV.dial CSV @20:59:50.86 CTV.dial :air @20:59:50.87 CS.seek 0ml @21:00:07.74 delay 7 seconds @21:00:14.77 CS.seek 3ml @21:00:22.87 CSV.dial :waste @21:00:23.31 CS.seek 0ml @21:00:31.07 CSV.dial :flush @21:00:31.50 CS.seek 3ml @21:00:39.59 delay 5 seconds @21:00:44.61 CSV.dial :air @21:00:45.11 CS.seek 5ml @21:00:50.84 CSV.dial :air @21:00:50.85 CS.seek 0ml @21:01:03.15 delay 7 seconds @21:01:10.18 CS.seek 3ml @21:01:18.24 CSV.dial :waste @21:01:18.98 CS.seek 0ml @21:01:26.68 Garaging FlushPuck in Collection Clamp @21:01:26.75 Elbow.reconfigure :tight @21:01:26.79 Elbow.seek CC @21:01:27.07 Elbow.reconfigure :loose @21:01:27.16 Forearm.seek CC @21:01:28.15 Hand.close @21:01:28.74 CC.seek :open @21:01:33.35 Forearm.seek :retract @21:01:34.36 Elbow.reconfigure :tight @21:01:34.40 Elbow.seek :garage @21:01:37.32 Elbow.reconfigure :loose @21:01:37.41 Forearm.reconfigure :tight @21:01:37.45 Forearm.seek :garage @21:01:38.47 Hand.open @21:01:39.24 Forearm.reconfigure :loose @21:01:39.28 Forearm.seek :retract @21:01:40.24 da sampling at most 100ml, wcr at most 100ml @21:01:40.27 Loading sh1 puck from tube 5 into Collection Clamp @21:01:40.28 DAfans.email "Started da in tube 5 at 21:01:40UTC15-Apr-23\n{1 => 33,\n 2 => 33,\n 3 => 33,\n 4 => 1,\n 5 => 32,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@20:57:23, 16% flow, 21.4C, 52% humidity, 15.0psia, 13.598V, 0.302A, 0.290A avg, 4.11W",Subject: "da sampling at most 100ml, wcr at most 100ml" @21:01:40.54
SC.reconfigure :tight @21:01:40.58 SC.seek 5 @21:01:44.16 SC.configure :limp @21:01:44.26 upsync @21:01:44.41
Elbow.reconfigure :tight @21:01:44.45 Elbow.seek 5 @21:01:46.35 Forearm.reconfigure :tight @21:01:46.40 Forearm.seek 5 + 450 counts @21:01:47.37 SE.reconfigure :pick @21:01:47.42 SE.seek :beamBreak @21:01:48.44 SE.configure :default @21:01:48.58 Elbow.seek 5 @21:01:48.76 Forearm.seek 5 + 300 counts @21:01:48.95 Forearm.reconfigure :loose @21:01:48.99 Hand.close @21:01:49.62 SE.seek :down @21:01:49.82
Forearm.seek :retract @21:01:50.29 SE.stop @21:01:50.75
Elbow.seek CC @21:01:52.38 Elbow.reconfigure :loose @21:01:52.47 Forearm.reconfigure :tight @21:01:52.52 Forearm.seek CC @21:01:53.74 CC.seek :closed @21:01:58.27 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.22 @21:01:58.28 Hand.open @21:01:58.92 Forearm.reconfigure :loose @21:01:58.96 Forearm.seek :retract @21:01:59.93 collectSample 100ml w/bubble point of 24psi @21:01:59.94 Priming 7ml @21:01:59.95 Intake.select :close @21:01:59.96 Exhaust.select :close @21:01:59.97 SS.hold @21:02:00.17 Calibrating pressure and temperature sensors @21:02:00.19 CTV.dial :air @21:02:00.21 SSV.dial :spit,avoiding: :exhaust @21:02:00.23 SS.reconfigure :fast @21:02:00.27 SS.seek 5ml @21:02:03.09 SSV.dial CBV @21:02:03.11
CBV.dial SSV @21:02:04.48 delay 3.8 seconds @21:02:08.39 SSV.dial :spit @21:02:09.72 SS.seek 0ml @21:02:12.52 SSV.dial CBV @21:02:13.84 CTV.dial :intake @21:02:14.63 SSV.dial CBV @21:02:14.66
CBV.dial SSV @21:02:14.67 Allowing 40s for 7.0ml of remaining 7.0ml @21:02:14.68 Intake.select :open @21:02:16.84 Intake @15.3psia, vacuum from 9.8 to 12.8psi @21:02:16.85 SS.reconfigure :inhaling 2.20ml/s @21:02:16.90 SS.seek 7ml @21:02:23.96 Stroke completed -- waiting for pressure to equalize ... @21:02:23.97 SS.reconfigure :exhale @21:02:24.01 SS.hold @21:02:25.20 Intake.select :close @21:02:25.34 SSV.dial :exhaust @21:02:26.10 Exhaust.select :open @21:02:26.21 SS.seek 0ml @21:02:30.44 Exhaust.select :close @21:02:30.54 Sampling 100.0ml @21:02:30.55 Intake.select :close @21:02:30.56 Exhaust.select :close @21:02:30.57 SS.reconfigure :default @21:02:30.61 SS.hold @21:02:30.80 CTV.dial :intake @21:02:30.81 SSV.dial CBV @21:02:30.84
CBV.dial SSV @21:02:31.60 Allowing 130s for 25.0ml of remaining 100.0ml @21:02:31.61 Intake.select :open @21:02:33.77 Intake @15.6psia, vacuum from 10.0 to 13.0psi @21:02:33.78 SS.reconfigure :inhaling 2.20ml/s @21:02:33.83 SS.seek 25ml @21:02:45.65 Vacuum too high -- waiting up to 40 seconds @21:02:45.66 SS.reconfigure :exhale @21:02:45.71 SS.hold @21:02:46.40 Intake @11.5psia, vacuum from 6.0 to 9.0psi @21:02:46.41 SS.reconfigure :inhaling 1.98ml/s @21:02:46.46 SS.seek 25ml @21:02:50.05 Stroke completed -- waiting for pressure to equalize ... @21:02:50.06 SS.reconfigure :exhale @21:02:50.10 SS.hold @21:02:54.59 Intake.select :close @21:02:54.74 SSV.dial :exhaust @21:02:55.49 Exhaust.select :open @21:02:55.60 SS.seek 0ml @21:03:04.49 Exhaust.select :close @21:03:04.59 SSV.dial CBV @21:03:04.64
CBV.dial SSV @21:03:05.38 Allowing 130s for 25.0ml of remaining 75.0ml @21:03:05.39 Intake.select :open @21:03:07.54 Intake @15.7psia, vacuum from 10.0 to 13.0psi @21:03:07.56 SS.reconfigure :inhaling 1.98ml/s @21:03:07.60 SS.seek 25ml @21:03:20.96 Vacuum too high -- waiting up to 40 seconds @21:03:20.97 SS.reconfigure :exhale @21:03:21.02 SS.hold @21:03:21.64 Intake @11.8psia, vacuum from 6.3 to 9.3psi @21:03:21.66 SS.reconfigure :inhaling 1.79ml/s @21:03:21.70 SS.seek 25ml @21:03:25.99 Stroke completed -- waiting for pressure to equalize ... @21:03:26.00 SS.reconfigure :exhale @21:03:26.04 SS.hold @21:03:31.66 Intake.select :close @21:03:31.80 SSV.dial :exhaust @21:03:32.57 Exhaust.select :open @21:03:32.67 SS.seek 0ml @21:03:41.42 Exhaust.select :close @21:03:41.53 SSV.dial CBV @21:03:41.56
CBV.dial SSV @21:03:42.32 Allowing 130s for 25.0ml of remaining 50.0ml @21:03:42.33 Intake.select :open @21:03:44.49 Intake @15.6psia, vacuum from 10.0 to 13.0psi @21:03:44.50 SS.reconfigure :inhaling 1.79ml/s @21:03:44.54 SS.seek 25ml @21:04:02.08 Stroke completed -- waiting for pressure to equalize ... @21:04:02.09 SS.reconfigure :exhale @21:04:02.13 SS.hold @21:04:08.56 Intake.select :close @21:04:08.70 SSV.dial :exhaust @21:04:09.46 Exhaust.select :open @21:04:09.56 SS.seek 0ml @21:04:18.36 Exhaust.select :close @21:04:18.46 SSV.dial CBV @21:04:18.49
CBV.dial SSV @21:04:19.25 Allowing 130s for 25.0ml of remaining 25.0ml @21:04:19.26 Intake.select :open @21:04:21.42 Intake @15.6psia, vacuum from 10.0 to 13.0psi @21:04:21.43 SS.reconfigure :inhaling 1.79ml/s @21:04:21.48 SS.seek 25ml @21:04:38.80 Stroke completed -- waiting for pressure to equalize ... @21:04:38.81 SS.reconfigure :exhale @21:04:38.85 SS.hold @21:04:45.26 Intake.select :close @21:04:45.39 SSV.dial :exhaust @21:04:46.17 Exhaust.select :open @21:04:46.28 SS.seek 0ml @21:04:55.06 Exhaust.select :close @21:04:55.17 Sampled 100.0ml @21:04:55.18 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @21:04:55.19 Intake.select :close @21:04:55.25 CSV.dial :waste @21:04:55.29 CS.seek 0ml @21:04:55.39 SSV.dial CBV,avoiding: :exhaust @21:04:55.42 CSV.dial :air @21:04:55.44
CBV.dial SSV @21:04:56.23 CS.seek 10ml @21:04:57.38
CTV.dial :air @21:04:58.18 delay 15 seconds @21:05:13.20 SSV.dial :spit,avoiding: :exhaust @21:05:14.55 CTV.dial CSV,avoiding: :intake @21:05:14.59
SS.seek 0ml @21:05:14.70 SSV.dial CBV,avoiding: :exhaust @21:05:21.42 CBV.dial between SSV and waste2 @21:05:21.80 CTV.dial CSV,avoiding: :air @21:05:21.83
CSV.dial CTV @21:05:22.29 CS.reconfigure :pressurize @21:05:22.33 CS.seek 0ml @21:05:40.09 BubblePoint: Intake @39.4psia, Outlet @15.2psia @21:05:40.10 CBV.dial SSV @21:05:40.43 SS.reconfigure :default @21:05:40.48 SS.seek 10ml @21:05:46.09 delay 15 seconds @21:06:01.21 Evacuated: Intake @16.3psia, Outlet @9.2psia @21:06:01.23 SSV.dial :spit,avoiding: :exhaust @21:06:02.95 SS.reconfigure :exhale @21:06:03.08 SS.seek 0ml @21:06:08.56 SS.reconfigure :fast @21:06:08.60 SS.seek 5ml @21:06:11.52 SSV.dial CBV,avoiding: :exhaust @21:06:11.56
CBV.dial SSV @21:06:12.88 CTV.dial :air,avoiding: :intake @21:06:14.29 CSV.dial CTV @21:06:14.32
SS.seek 10ml @21:06:15.32 CSV.dial :air @21:06:15.79 CS.reconfigure :fast @21:06:15.84 CS.seek 0ml @21:06:16.78
SSV.dial :spit,avoiding: :exhaust @21:06:18.12 SS.reconfigure :exhale @21:06:18.16 SS.seek 0ml @21:06:23.67 SS.reconfigure :fast @21:06:23.71 SS.seek 5ml @21:06:24.24 CS.stop @21:06:26.52 SSV.dial CBV,avoiding: :exhaust @21:06:26.55
CBV.dial SSV @21:06:28.90 SSV.dial :spit,avoiding: :exhaust @21:06:30.22 SS.reconfigure :default @21:06:30.27 SS.seek 0ml @21:06:33.75 SS.stop @21:06:33.78 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @21:06:33.79 Intake.select :close @21:06:33.87 CSV.dial :waste @21:06:33.98 SSV.dial CBV,avoiding: :exhaust @21:06:34.00
CBV.dial SSV @21:06:34.65 CS.seek 0ml @21:06:34.71 CSV.dial :air @21:06:35.35
CTV.dial :air @21:06:35.36 delay 15 seconds @21:06:35.45 CS.seek 10ml @21:06:50.38
SSV.dial :spit,avoiding: :exhaust @21:06:51.71 CTV.dial CSV,avoiding: :intake @21:06:51.73
SS.reconfigure :exhale @21:06:51.83 SS.seek 0ml @21:06:52.25 SSV.dial CBV,avoiding: :exhaust @21:07:00.11 CBV.dial between SSV and waste2 @21:07:00.50 CTV.dial CSV,avoiding: :air @21:07:00.53
CSV.dial CTV @21:07:00.98 CS.reconfigure :pressurize @21:07:01.02 CS.seek 0ml @21:07:22.21 BubblePoint: Intake @38.3psia, Outlet @14.1psia @21:07:22.22 CBV.dial SSV @21:07:22.55 SS.reconfigure :default @21:07:22.60 SS.seek 10ml @21:07:28.19 delay 15 seconds @21:07:43.31 Evacuated: Intake @18.1psia, Outlet @11.7psia @21:07:43.32 SSV.dial :spit,avoiding: :exhaust @21:07:44.66 SS.reconfigure :exhale @21:07:44.70 SS.seek 0ml @21:07:50.12 SS.reconfigure :fast @21:07:50.16 SS.seek 5ml @21:07:53.00 SSV.dial CBV,avoiding: :exhaust @21:07:53.03
CBV.dial SSV @21:07:54.36 CTV.dial :air,avoiding: :intake @21:07:55.75 CSV.dial CTV @21:07:55.79
SS.seek 10ml @21:07:56.79 CSV.dial :air @21:07:57.26 CS.reconfigure :fast @21:07:57.30 CS.seek 0ml @21:07:58.25
SSV.dial :spit,avoiding: :exhaust @21:07:59.58 SS.reconfigure :exhale @21:07:59.63 SS.seek 0ml @21:08:02.55 CS.stop @21:08:04.94
SS.reconfigure :fast @21:08:04.99 SS.seek 5ml @21:08:07.95 SSV.dial CBV,avoiding: :exhaust @21:08:07.97
CBV.dial SSV @21:08:10.30 SSV.dial :spit,avoiding: :exhaust @21:08:11.66 SS.reconfigure :default @21:08:11.70 SS.seek 0ml @21:08:14.87 SS.stop @21:08:14.91 Lysing: Adding 2.0ml AqMeOH @21:08:14.92 CBV.dial between CSV and waste1,avoiding: :DBV @21:08:16.46 CTV.dial CSV @21:08:17.81 CSV.dial CSR @21:08:18.62 Pulling 1ml of CSR.AqMeOH CSR.select :AqMeOH @21:08:18.73 CS.reconfigure :default @21:08:18.77 CS.seek 1ml @21:08:22.46 delay 5 seconds @21:08:27.47 CSR.select :air @21:08:27.63 CS.seek 6ml @21:08:41.27 delay 2 seconds @21:08:43.28 CSV.dial :waste2 @21:08:43.71 CS.reconfigure :fast @21:08:43.75 CS.seek 0ml @21:08:58.48 CSV.dial CSR @21:08:58.98 Pulling 2ml of CSR.AqMeOH @21:08:58.99 CSR.select :AqMeOH @21:08:59.10 CS.reconfigure :default @21:08:59.14 CS.seek 2ml @21:09:05.05 delay 5 seconds @21:09:10.06 CSR.select :air @21:09:10.21 CS.seek 5ml @21:09:18.92 delay 2 seconds @21:09:20.94 CSV.dial CTV @21:09:21.40 CS.seek 1ml @21:09:32.56 Before lysing Collection Puck Heater at 21.4C/38.7psia @21:09:32.58 Heating Puck.from 5, :sh1 in Collection Clamp to 70C for 10:00 @21:09:32.59 CH.seek 70C,8:00 @21:11:33.51 CH.hold 10:00,70C @21:11:33.68 Begun lysing Collection Puck Heater at 68.1C/46.1psia @21:21:33.70 Ended lysing Collection Puck Heater at 70.1C/43.8psia @21:21:33.72 CS.seek 0ml @21:21:37.48 CTV.dial between spare and air,avoiding: :intake @21:21:38.52 CSV.dial :air @21:21:38.97 CS.seek 5ml @21:21:52.56 CSV.dial CTV @21:21:53.00 delay 2 seconds @21:21:55.02 CSV.dial :air @21:21:55.47 delay 2 seconds @21:21:57.49 CSV.dial CTV @21:21:57.95 delay 2 seconds @21:21:59.96 CSV.dial :air @21:22:00.41 delay 2 seconds @21:22:02.43 CS.seek 0ml @21:22:16.12 Puck @71.8psia after final lysis pressurization @21:22:16.13 Recovering Lysate into Collection syringe @21:22:16.14 CSV.dial CBV @21:22:17.16 CBV.dial CSV @21:22:17.53 CS.seek 5ml @21:22:31.09 delay 5 seconds @21:22:36.11 CTV.dial :air @21:22:36.64 CS.seek 7ml @21:22:42.63 delay 5 seconds @21:22:47.64 CSV.dial :air2 @21:22:48.08 CS.seek 10ml @21:22:56.66 CSV.dial between air and CTV,avoiding: CBV @21:22:57.83 Lysing Complete @21:22:57.90 Storing sh1 puck in Collection Clamp to tube 4 @21:22:58.06 SC.reconfigure :tight @21:22:58.10 SC.seek 4 @21:23:01.66 SC.configure :limp @21:23:01.86 Elbow.reconfigure :tight @21:23:01.90 Elbow.seek 4 @21:23:03.67 Forearm.reconfigure :tight @21:23:03.71 Forearm.seek 4 + 450 counts @21:23:04.71 SE.reconfigure :pick @21:23:04.76 SE.seek :beamBreak @21:23:07.80 SE.configure :default @21:23:07.94 Elbow.reconfigure :limp @21:23:07.99 Forearm.reconfigure :loose @21:23:08.02 Forearm.seek 4 @21:23:08.24 Elbow.hold @21:23:08.30 Elbow.reconfigure :tight @21:23:08.39 SE.seek 0.18 Pucks below Top Plate @21:23:08.83 Forearm.force -20 @21:23:08.87 Forearm.seek 4 + 288 counts @21:23:09.16 Forearm.reconfigure :tight @21:23:09.20 Forearm.seek 4 + 450 counts @21:23:09.41 SE.seek 1.18 Pucks below Top Plate @21:23:09.90 Forearm.reconfigure :loose @21:23:09.94 Forearm.seek :retract @21:23:10.87 Elbow.seek CC @21:23:12.58 Elbow.reconfigure :loose @21:23:12.67 Forearm.seek CC @21:23:13.65 Hand.close @21:23:14.22 CC.seek :open @21:23:18.97 Forearm.seek :retract @21:23:19.93 Elbow.reconfigure :tight @21:23:19.98 Elbow.seek 4 @21:23:22.15 Elbow.reconfigure :limp @21:23:22.21 Forearm.seek 4 @21:23:23.17 Elbow.hold @21:23:23.23 Elbow.reconfigure :tight @21:23:23.27 Hand.open @21:23:23.96 Forearm.force -20 @21:23:24.00 Forearm.seek 4 + 317 counts @21:23:24.28 Forearm.reconfigure :tight @21:23:24.32 Forearm.seek 4 + 450 counts @21:23:24.59 SE.seek :down @21:23:25.72
Forearm.reconfigure :loose @21:23:25.76 Forearm.seek :retract @21:23:27.20 SE.stop @21:23:27.24
Tranferring DA @21:23:27.25 Beginning samplePassC @21:23:27.26 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @21:23:27.45 Elbow.seek FlushPuck::Garage @21:23:29.30 Elbow.reconfigure :loose @21:23:29.39 Forearm.seek :garage @21:23:30.40 Hand.close @21:23:31.08 Forearm.seek :retract @21:23:32.04 Elbow.reconfigure :tight @21:23:32.08 Elbow.seek CC @21:23:34.99 Elbow.reconfigure :loose @21:23:35.09 Forearm.reconfigure :tight @21:23:35.13 Forearm.seek CC @21:23:36.36 CC.seek :closed @21:23:40.92 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.40 @21:23:40.99 Hand.open @21:23:41.65 Forearm.reconfigure :loose @21:23:41.69 Forearm.seek :retract @21:23:42.63 PSV.dial PSR @21:23:44.02 Pulling 1.5ml of PSR.flush @21:23:44.03 PSR.select :flush @21:23:44.15 PS.seek 1.5ml @21:24:00.49 delay 5 seconds @21:24:05.50 PSV.dial :waste1 @21:24:05.96 PS.seek 0ml @21:24:22.10 PSV.dial PSR @21:24:22.56 PSR.select :air @21:24:22.67 PS.seek 2.5ml @21:24:48.98 PRV.dial CBV,avoiding: :ab2 @21:24:49.01
CBV.dial PRV @21:24:50.70 PSV.dial :mixloop @21:24:51.75 CTV.dial :air,avoiding: :intake @21:24:51.76 PTV.dial :PRVmixing @21:24:53.10 PS.seek 0ml @21:25:19.51 PSV.dial :air @21:25:19.97 PS.seek 2.5ml @21:25:46.32 PSV.dial :mixloop @21:25:46.80 PS.seek 0ml @21:26:13.10 PSV.dial :air @21:26:13.56 PS.seek 2.5ml @21:26:39.95 PSV.dial :mixloop @21:26:40.41 PS.seek 0ml @21:27:06.92 Garaging FlushPuck in Collection Clamp @21:27:06.99 Elbow.reconfigure :tight @21:27:07.03 Elbow.seek CC @21:27:07.34 Elbow.reconfigure :loose @21:27:07.43 Forearm.seek CC @21:27:08.43 Hand.close @21:27:09.09 CC.seek :open @21:27:13.70 Forearm.seek :retract @21:27:14.67 Elbow.reconfigure :tight @21:27:14.71 Elbow.seek :garage @21:27:17.59 Elbow.reconfigure :loose @21:27:17.68 Forearm.reconfigure :tight @21:27:17.72 Forearm.seek :garage @21:27:18.78 Hand.open @21:27:19.90 Forearm.reconfigure :loose @21:27:19.94 Forearm.seek :retract @21:27:20.91 PSV.dial :mixloop @21:27:20.93 PTV.dial :PRVmixing @21:27:20.94 PRV.dial CBV @21:27:20.96
CBV.dial PRV @21:27:20.98 CTV.dial :air,avoiding: :intake @21:27:21.00 Loading lyfil puck from tube 5 into Collection Clamp @21:27:21.21 SC.reconfigure :tight @21:27:21.26 SC.seek 5 @21:27:24.77 SC.configure :limp @21:27:24.96 Elbow.reconfigure :tight @21:27:25.00 Elbow.seek 5 @21:27:26.90 Forearm.reconfigure :tight @21:27:26.94 Forearm.seek 5 + 450 counts @21:27:27.94 SE.reconfigure :pick @21:27:27.99 SE.seek :beamBreak @21:27:29.05 SE.configure :default @21:27:29.20 Elbow.seek 5 @21:27:29.37 Forearm.seek 5 + 300 counts @21:27:29.72 Forearm.reconfigure :loose @21:27:29.77 Hand.close @21:27:30.35 SE.seek :down @21:27:30.55
Forearm.seek :retract @21:27:31.09 SE.stop @21:27:31.46
Elbow.seek CC @21:27:33.05 Elbow.reconfigure :loose @21:27:33.14 Forearm.reconfigure :tight @21:27:33.18 Forearm.seek CC @21:27:34.43 CC.seek :closed @21:27:38.95 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.17 @21:27:38.97 Hand.open @21:27:39.65 Forearm.reconfigure :loose @21:27:39.69 Forearm.seek :retract @21:27:40.65 CTV.dial CSV,avoiding: :intake @21:27:42.01 CSV.dial CTV @21:27:42.33 PS.seek 2.5ml @21:28:08.75 CS.seek 4.5ml @21:28:23.68 delay 8 seconds @21:28:31.70 PSV.dial :air @21:28:32.17 delay 2 seconds @21:28:34.18 PSV.dial :waste2 @21:28:34.94 PS.seek 0ml @21:29:01.35 CBV.dial between PRV and waste1 @21:29:01.68 CSV.dial :air @21:29:02.27 CS.seek 10ml @21:29:17.12 CSV.dial CTV @21:29:17.55 CS.seek 0ml @21:29:44.02 CBV.dial :waste1 @21:29:44.31 delay 5 seconds @21:29:49.33 CTV.dial :air,avoiding: :intake @21:29:50.74 Storing lyfil puck in Collection Clamp to tube 4 @21:29:50.90 SC.reconfigure :tight @21:29:50.94 SC.seek 4 @21:29:54.60 SC.configure :limp @21:29:54.79 Elbow.reconfigure :tight @21:29:54.84 Elbow.seek 4 @21:29:56.63 Forearm.reconfigure :tight @21:29:56.68 Forearm.seek 4 + 450 counts @21:29:57.77 SE.reconfigure :pick @21:29:57.82 SE.seek :beamBreak @21:30:00.86 SE.configure :default @21:30:01.46 Elbow.reconfigure :limp @21:30:01.51 Forearm.reconfigure :loose @21:30:01.55 Forearm.seek 4 @21:30:01.75 Elbow.hold @21:30:01.81 Elbow.reconfigure :tight @21:30:01.90 SE.seek 1.19 Pucks below Top Plate @21:30:02.45 Forearm.force -20 @21:30:02.49 Forearm.seek 4 + 312 counts @21:30:02.77 Forearm.reconfigure :tight @21:30:02.81 Forearm.seek 4 + 450 counts @21:30:03.03 SE.seek 2.19 Pucks below Top Plate @21:30:03.57 Forearm.reconfigure :loose @21:30:03.62 Forearm.seek :retract @21:30:04.55 Elbow.seek CC @21:30:06.19 Elbow.reconfigure :loose @21:30:06.28 Forearm.seek CC @21:30:07.27 Hand.close @21:30:07.83 CC.seek :open @21:30:12.55 Forearm.seek :retract @21:30:13.52 Elbow.reconfigure :tight @21:30:13.56 Elbow.seek 4 @21:30:15.33 Elbow.reconfigure :limp @21:30:15.38 Forearm.seek 4 @21:30:16.38 Elbow.hold @21:30:16.44 Elbow.reconfigure :tight @21:30:16.48 Hand.open @21:30:17.19 Forearm.force -20 @21:30:17.23 Forearm.seek 4 + 314 counts @21:30:17.64 Forearm.reconfigure :tight @21:30:17.68 Forearm.seek 4 + 450 counts @21:30:17.94 SE.seek :down @21:30:19.07
Forearm.reconfigure :loose @21:30:19.11 Forearm.seek :retract @21:30:20.54 SE.stop @21:30:20.58
PSV.dial :air @21:30:21.33 PS.seek 2.5ml @21:30:47.69 delay 1 second @21:30:48.71 PSV.dial :mixloop @21:30:49.15 delay 1 second @21:30:50.17 PSV.dial :air @21:30:50.63 delay 1 second @21:30:51.65 PSV.dial :mixloop @21:30:52.12 delay 1 second @21:30:53.13 PSV.dial :air @21:30:53.58 delay 1 second @21:30:54.60 PS.seek 0ml @21:31:20.91 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @21:31:21.09 Elbow.seek FlushPuck::Garage @21:31:22.90 Elbow.reconfigure :loose @21:31:22.99 Forearm.seek :garage @21:31:24.02 Hand.close @21:31:24.69 Forearm.seek :retract @21:31:25.68 Elbow.reconfigure :tight @21:31:25.72 Elbow.seek PC @21:31:26.98 Elbow.reconfigure :loose @21:31:27.07 Forearm.reconfigure :tight @21:31:27.11 Forearm.seek PC @21:31:28.32 PC.seek :closed @21:31:32.94 Processing Clamp onSpring/offSpring for 16/26 samples, on/off torque 1.53 @21:31:33.01 Hand.open @21:31:33.64 Forearm.reconfigure :loose @21:31:33.68 Forearm.seek :retract @21:31:35.09 PRV.dial :air @21:31:35.55 PSV.dial :mixloop @21:31:36.08 PS.seek 0.3ml @21:31:40.03 PSV.dial PSR @21:31:41.14 Pulling 0.4ml of PSR.air @21:31:41.15 PSR.select :air @21:31:41.17 PS.seek 0.7ml @21:31:46.04 delay 3 seconds @21:31:49.06 PSR.select :air @21:31:49.08 PS.seek 2.5ml @21:32:08.32 delay 3 seconds @21:32:11.33 PSV.dial :mixloop @21:32:12.52 PTV.dial :mixing @21:32:13.28 PBV.dial :waste2 @21:32:14.23 PS.seek 0ml @21:32:40.63 Garaging FlushPuck in Processing Clamp @21:32:40.70 Elbow.reconfigure :tight @21:32:40.74 Elbow.seek PC @21:32:41.06 Elbow.reconfigure :loose @21:32:41.15 Forearm.seek PC @21:32:42.11 Hand.close @21:32:42.71 PC.seek :open @21:32:47.46 Forearm.seek :retract @21:32:48.44 Elbow.reconfigure :tight @21:32:48.48 Elbow.seek :garage @21:32:49.79 Elbow.reconfigure :loose @21:32:49.93 Forearm.reconfigure :tight @21:32:49.97 Forearm.seek :garage @21:32:51.00 Hand.open @21:32:51.64 Forearm.reconfigure :loose @21:32:51.68 Forearm.seek :retract @21:32:52.67 Loading da puck from tube 5 into Processing Clamp @21:32:52.89 SC.reconfigure :tight @21:32:52.93 SC.seek 5 @21:32:56.45 SC.configure :limp @21:32:56.64 Elbow.reconfigure :tight @21:32:56.68 Elbow.seek 5 @21:32:58.54 Forearm.reconfigure :tight @21:32:58.58 Forearm.seek 5 + 450 counts @21:32:59.62 SE.reconfigure :pick @21:32:59.67 SE.seek :beamBreak @21:33:00.83 SE.configure :default @21:33:00.97 Elbow.seek 5 @21:33:01.14 Forearm.seek 5 + 300 counts @21:33:01.56 Forearm.reconfigure :loose @21:33:01.61 Hand.close @21:33:02.22 SE.seek :down @21:33:02.41
Forearm.seek :retract @21:33:03.01 SE.stop @21:33:03.35
Elbow.seek PC @21:33:05.87 Elbow.reconfigure :loose @21:33:05.96 Forearm.reconfigure :tight @21:33:06.00 Forearm.seek PC @21:33:07.17 PC.seek :closed @21:33:11.78 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.58 @21:33:11.79 Hand.open @21:33:12.51 Forearm.reconfigure :loose @21:33:12.56 Forearm.seek :retract @21:33:13.51 Heating Puck.from 5, :da in Processing Clamp to 28C for 2:00:00 @21:33:13.53 PH.seek 28C,5:00 @21:33:43.41 PH.hold 2:00:00,28C @21:33:43.45 delay 1:30 @21:35:13.48 PSR.select :air @21:35:13.50 PSV.dial PSR @21:35:14.59 Pulling 0.5ml of PSR.wash @21:35:14.60 PSR.select :wash @21:35:14.71 PS.seek 0.5ml @21:35:20.81 delay 5 seconds @21:35:25.83 PSR.select :air @21:35:25.95 PS.seek 2ml @21:35:42.09 PTV.dial :puck @21:35:42.84 PSV.dial PTV @21:35:43.61 PBV.dial :waste1 @21:35:45.00 PS.seek 1.5ml @21:35:50.91 delay 3 seconds @21:35:53.92 PBV.dial between PSV and waste1 @21:35:54.29 PS.seek 0ml @21:36:10.44 delay 5:00 @21:41:10.46 recoverPSRreagent08 @21:41:10.47 PTV.dial :puck @21:41:10.48 PBV.dial :waste1 @21:41:10.85 delay 30 seconds @21:41:40.88 PBV.dial between PSV and waste1 @21:41:41.24 PSR.select :air @21:41:41.26 PSV.dial PSR @21:41:42.03 PS.reconfigure :fast @21:41:42.07 PS.seek 2.5ml @21:42:05.96 PSV.dial PTV @21:42:06.71 delay 5 seconds @21:42:11.73 PSV.dial PSR @21:42:12.49 delay 5 seconds @21:42:17.51 PSV.dial PTV @21:42:18.27 delay 5 seconds @21:42:23.29 PSV.dial :air @21:42:24.04 PS.seek 0ml @21:42:47.34 PTV.dial :PRVmixing,via: PRV @21:42:48.66 PSV.dial :mixloop @21:42:49.13 PRV.dial :ab1 @21:42:49.90 PS.reconfigure :slow @21:42:49.95 PS.seek 0.15ml @21:42:53.49 delay 2 seconds @21:42:55.51 CBV.dial PRV @21:42:55.54
PRV.dial CBV @21:42:56.10 PS.seek 0.35ml @21:43:00.65 delay 2 seconds @21:43:02.66 CBV.dial between PRV and PSV @21:43:02.97 PRV.dial :air @21:43:03.42 PS.seek 0.9ml @21:43:15.18 PRV.dial :waste2 @21:43:15.92 PS.seek 0.5ml @21:43:24.62 addPMLReagent08 PSV.dial :air @21:43:25.09 PS.reconfigure :default @21:43:25.13 PS.seek 2ml @21:43:41.24 PSV.dial :PML @21:43:41.69 PTV.dial :mixing @21:43:42.43 PBV.dial :waste1 @21:43:42.85 PS.seek 1.85ml @21:43:45.14 PBV.dial between PSV and waste1 @21:43:45.53 PS.seek 0ml @21:44:05.18 delay 2 seconds @21:44:07.20 Beginning samplePassC @21:44:07.21 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @21:44:07.37 Elbow.reconfigure :tight @21:44:07.41 Elbow.seek FlushPuck::Garage @21:44:08.75 Elbow.reconfigure :loose @21:44:08.84 Forearm.seek :garage @21:44:09.87 Hand.close @21:44:10.56 Forearm.seek :retract @21:44:11.57 Elbow.reconfigure :tight @21:44:11.62 Elbow.seek CC @21:44:14.48 Elbow.reconfigure :loose @21:44:14.57 Forearm.reconfigure :tight @21:44:14.61 Forearm.seek CC @21:44:15.82 CC.seek :closed @21:44:20.35 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.40 @21:44:20.42 Hand.open @21:44:21.60 Forearm.reconfigure :loose @21:44:21.64 Forearm.seek :retract @21:44:22.60 PSV.dial PSR @21:44:23.72 Pulling 1.5ml of PSR.flush @21:44:23.73 PSR.select :flush @21:44:23.84 PS.seek 1.5ml @21:44:39.99 delay 5 seconds @21:44:45.01 PSV.dial :waste1 @21:44:45.46 PS.seek 0ml @21:45:01.55 PSV.dial PSR @21:45:02.00 PSR.select :air @21:45:02.12 PS.seek 2.5ml @21:45:28.43 PRV.dial CBV,avoiding: :ab2 @21:45:28.46
CBV.dial PRV @21:45:30.07 PSV.dial :mixloop @21:45:31.11 CTV.dial :air,avoiding: :intake @21:45:31.13 PTV.dial :PRVmixing @21:45:31.88 PS.seek 0ml @21:45:58.27 PSV.dial :air @21:45:58.73 PS.seek 2.5ml @21:46:25.08 PSV.dial :mixloop @21:46:25.56 PS.seek 0ml @21:46:51.86 PSV.dial :air @21:46:52.44 PS.seek 2.5ml @21:47:18.80 PSV.dial :mixloop @21:47:19.24 PS.seek 0ml @21:47:45.55 Garaging FlushPuck in Collection Clamp @21:47:45.62 Elbow.reconfigure :tight @21:47:45.66 Elbow.seek CC @21:47:45.78 Elbow.reconfigure :loose @21:47:45.87 Forearm.seek CC @21:47:46.85 Hand.close @21:47:47.45 CC.seek :open @21:47:52.09 Forearm.seek :retract @21:47:53.10 Elbow.reconfigure :tight @21:47:53.14 Elbow.seek :garage @21:47:56.12 Elbow.reconfigure :loose @21:47:56.21 Forearm.reconfigure :tight @21:47:56.26 Forearm.seek :garage @21:47:57.27 Hand.open @21:47:58.01 Forearm.reconfigure :loose @21:47:58.05 Forearm.seek :retract @21:47:59.01 delayUntil "21:52:07.18" @21:47:59.03 Whole Cell Archive sampling 100ml @21:47:59.05 Loading wcr puck from tube 5 into Collection Clamp @21:47:59.49 Elbow.reconfigure :tight @21:47:59.54 Elbow.seek 5 @21:48:01.43 Forearm.reconfigure :tight @21:48:01.48 Forearm.seek 5 + 450 counts @21:48:02.49 SE.reconfigure :pick @21:48:02.54 SE.seek :beamBreak @21:48:03.80 SE.configure :default @21:48:03.95 Elbow.seek 5 @21:48:04.12 Forearm.seek 5 + 300 counts @21:48:04.31 Forearm.reconfigure :loose @21:48:04.36 Hand.close @21:48:04.96 SE.seek :down @21:48:05.14 Forearm.seek :retract @21:48:05.89 SE.stop @21:48:06.09 Elbow.seek CC @21:48:07.66 Elbow.reconfigure :loose @21:48:07.76 Forearm.reconfigure :tight @21:48:07.80 Forearm.seek CC @21:48:09.08 CC.seek :closed @21:48:13.65 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.17 @21:48:13.66 Hand.open @21:48:14.38 Forearm.reconfigure :loose @21:48:14.43 Forearm.seek :retract @21:48:15.43 collectSample 100ml w/bubble point of 35psi @21:48:15.44 Priming 7ml @21:48:15.45 Intake.select :close @21:48:15.46 Exhaust.select :close @21:48:15.47 SS.hold @21:48:15.68 Calibrating pressure sensors @21:48:15.69 CTV.dial :air @21:48:15.70 SSV.dial :spit,avoiding: :exhaust @21:48:15.72 SS.reconfigure :fast @21:48:15.76 SS.seek 5ml @21:48:18.47 SSV.dial CBV @21:48:18.50 CBV.dial SSV @21:48:19.84 delay 3.8 seconds @21:48:23.77 SSV.dial :spit @21:48:25.11 SS.seek 0ml @21:48:27.95 SSV.dial CBV @21:48:29.30 CTV.dial :intake @21:48:30.07 SSV.dial CBV @21:48:30.10 CBV.dial SSV @21:48:30.11 Allowing 40s for 7.0ml of remaining 7.0ml @21:48:30.12 Intake.select :open @21:48:32.28 Intake @15.5psia, vacuum from 10.0 to 13.0psi @21:48:32.30 SS.reconfigure :inhaling 2.20ml/s @21:48:32.34 SS.seek 7ml @21:48:39.06 Stroke completed -- waiting for pressure to equalize ... @21:48:39.07 SS.reconfigure :exhale @21:48:39.11 SS.hold @21:48:55.31 Intake.select :close @21:48:55.46 SSV.dial :exhaust @21:48:56.21 Exhaust.select :open @21:48:56.32 SS.seek 0ml @21:49:00.70 Exhaust.select :close @21:49:00.80 Sampling 100.0ml @21:49:00.81 Intake.select :close @21:49:00.82 Exhaust.select :close @21:49:00.84 SS.reconfigure :default @21:49:00.87 SS.hold @21:49:01.08 CTV.dial :intake @21:49:01.09 SSV.dial CBV @21:49:01.12 CBV.dial SSV @21:49:01.90 Allowing 130s for 25.0ml of remaining 100.0ml @21:49:01.92 Intake.select :open @21:49:04.07 Intake @16.4psia, vacuum from 10.0 to 13.0psi @21:49:04.09 SS.reconfigure :inhaling 2.20ml/s @21:49:04.13 SS.seek 25ml @21:49:08.91 Vacuum too high -- waiting up to 40 seconds @21:49:08.92 SS.reconfigure :exhale @21:49:08.96 SS.hold @21:49:11.76 Intake @15.0psia, vacuum from 9.5 to 12.5psi @21:49:11.78 SS.reconfigure :inhaling 1.98ml/s @21:49:11.83 SS.seek 25ml @21:49:19.50 Vacuum too high -- waiting up to 40 seconds @21:49:19.51 SS.reconfigure :exhale @21:49:19.56 SS.hold @21:49:26.37 Intake @15.8psia, vacuum from 10.0 to 13.0psi @21:49:26.39 SS.reconfigure :inhaling 1.79ml/s @21:49:26.43 SS.seek 25ml @21:49:31.02 Stroke completed -- waiting for pressure to equalize ... @21:49:31.04 SS.reconfigure :exhale @21:49:31.08 SS.hold @21:49:35.86 Intake.select :close @21:49:36.01 SSV.dial :exhaust @21:49:36.78 Exhaust.select :open @21:49:36.89 SS.seek 0ml @21:49:45.52 Exhaust.select :close @21:49:45.63 SSV.dial CBV @21:49:45.66 CBV.dial SSV @21:49:46.43 Allowing 130s for 25.0ml of remaining 75.0ml @21:49:46.45 Intake.select :open @21:49:48.61 Intake @16.3psia, vacuum from 10.0 to 13.0psi @21:49:48.62 SS.reconfigure :inhaling 1.79ml/s @21:49:48.67 SS.seek 25ml @21:49:57.56 Vacuum too high -- waiting up to 40 seconds @21:49:57.58 SS.reconfigure :exhale @21:49:57.62 SS.hold @21:49:59.84 Intake @14.5psia, vacuum from 9.0 to 12.0psi @21:49:59.85 SS.reconfigure :inhaling 1.61ml/s @21:49:59.90 SS.seek 25ml @21:50:09.91 Stroke completed -- waiting for pressure to equalize ... @21:50:09.93 SS.reconfigure :exhale @21:50:09.97 SS.hold @21:50:17.85 Intake.select :close @21:50:17.99 SSV.dial :exhaust @21:50:18.77 Exhaust.select :open @21:50:18.89 SS.seek 0ml @21:50:27.85 Exhaust.select :close @21:50:27.96 SSV.dial CBV @21:50:27.99 CBV.dial SSV @21:50:28.74 Allowing 130s for 25.0ml of remaining 50.0ml @21:50:28.76 Intake.select :open @21:50:30.92 Intake @16.3psia, vacuum from 10.0 to 13.0psi @21:50:30.93 SS.reconfigure :inhaling 1.61ml/s @21:50:30.98 SS.seek 25ml @21:50:43.69 Vacuum too high -- waiting up to 40 seconds @21:50:43.70 SS.reconfigure :exhale @21:50:43.74 SS.hold @21:50:44.53 Intake @13.6psia, vacuum from 8.1 to 11.1psi @21:50:44.54 SS.reconfigure :inhaling 1.45ml/s @21:50:44.59 SS.seek 25ml @21:50:51.57 Stroke completed -- waiting for pressure to equalize ... @21:50:51.58 SS.reconfigure :exhale @21:50:51.62 SS.hold @21:50:56.25 Intake.select :close @21:50:56.39 SSV.dial :exhaust @21:50:57.15 Exhaust.select :open @21:50:57.26 SS.seek 0ml @21:51:06.12 Exhaust.select :close @21:51:06.22 SSV.dial CBV @21:51:06.25 CBV.dial SSV @21:51:07.53 Allowing 130s for 25.0ml of remaining 25.0ml @21:51:07.54 Intake.select :open @21:51:09.69 Intake @16.3psia, vacuum from 10.0 to 13.0psi @21:51:09.71 SS.reconfigure :inhaling 1.45ml/s @21:51:09.76 SS.seek 24.99ml @21:51:28.06 Vacuum too high -- waiting up to 40 seconds @21:51:28.07 SS.reconfigure :exhale @21:51:28.12 SS.hold @21:51:28.61 Intake @13.6psia, vacuum from 8.1 to 11.1psi @21:51:28.63 SS.reconfigure :inhaling 1.30ml/s @21:51:28.67 SS.seek 24.99ml @21:51:31.69 Stroke completed -- waiting for pressure to equalize ... @21:51:31.70 SS.reconfigure :exhale @21:51:31.75 SS.hold @21:51:35.83 Intake.select :close @21:51:35.98 SSV.dial :exhaust @21:51:36.76 Exhaust.select :open @21:51:36.87 SS.seek 0ml @21:51:45.91 Exhaust.select :close @21:51:46.02 Sampled 100.0ml @21:51:46.04 Drying puck before fixing for 20:00 CBV.dial :waste2b @21:51:46.80 delay 1:00 @21:52:07.19 recoverPRVreagent08 @21:52:07.21 PTV.dial :mixing @21:52:07.96 PBV.dial :waste1 @21:52:08.34 delay 30 seconds @21:52:38.37 PBV.dial between PSV and waste1 @21:52:38.73 PSV.dial :air @21:52:39.33 PS.reconfigure :fast @21:52:39.38 PS.seek 2.5ml @21:52:46.82 CSV.dial :air @21:52:47.27 CS.reconfigure :fast @21:52:47.32 CS.seek 10ml @21:53:03.51 PSV.dial :mixloop @21:53:03.96 delay 2 seconds @21:53:05.98 PSV.dial :air @21:53:06.45 delay 2 seconds @21:53:08.47 PSV.dial :mixloop @21:53:08.93 delay 2 seconds @21:53:10.95 PSV.dial :air @21:53:11.42 PS.seek 0ml @21:53:12.29 Pumping wcr puck from tube 5 up to 35psi... @21:53:12.30 CBV.dial SSV @21:53:13.62 CBV.dial between CSV and waste2b @21:53:14.58 CSV.dial between air and CTV @21:53:14.90 CS.seek 7.298ml @21:53:21.91 CTV.dial CSV,avoiding: :air @21:53:21.94 CSV.dial CTV @21:53:22.74 CS.reconfigure :pressurize @21:53:22.79 CS.seek 0ml @21:53:35.23 washCycleDA #1 of 2 @21:53:35.24 PSR.select :air @21:53:35.25 PSV.dial PSR @21:53:36.63 Pulling 0.5ml of PSR.wash @21:53:36.64 PSR.select :wash @21:53:36.74 CBV.dial :waste2b @21:53:36.80 PS.reconfigure :default @21:53:36.85 PS.seek 0.5ml @21:53:37.07 CS.reconfigure :default @21:53:37.12 CS.seek 0ml @21:53:42.76 delay 5 seconds @21:53:43.07 delay 1:00 @21:53:47.78 PSR.select :air @21:53:47.89 PS.seek 2ml @21:54:04.04 PTV.dial :puck @21:54:04.79 PSV.dial PTV @21:54:05.54 PBV.dial :waste1 @21:54:05.97 PS.seek 1.5ml @21:54:11.95 delay 3 seconds @21:54:14.96 PBV.dial between PSV and waste1 @21:54:15.34 PS.seek 0ml @21:54:31.51 delay 2:00 @21:54:43.08 CTV.dial :air,avoiding: :intake @21:54:44.43 Fix #1 of 2 (with RNAlater) for 10:00 @21:54:44.44 CSV.dial CSR @21:54:44.93 Pulling 2ml of CSR.RNAlater @21:54:44.94 CSR.select :RNAlater @21:54:45.05 CS.seek 2ml @21:54:50.97 delay 5 seconds @21:54:55.99 CSR.select :air @21:54:56.10 CS.reconfigure :fast @21:54:56.59 CS.seek 10ml @21:55:16.44 Pumping wcr puck from tube 5 up to 35psi... @21:55:16.45 CBV.dial SSV @21:55:17.78 CBV.dial between CSV and waste2b @21:55:18.75 CSV.dial between air and CTV @21:55:19.36 CS.seek 7.281ml @21:55:26.44 CTV.dial CSV,avoiding: :air @21:55:26.47 CSV.dial CTV @21:55:27.86 CS.reconfigure :pressurize @21:55:27.91 CS.seek 0ml @21:55:44.74 delay 10:00 @21:56:31.53 recoverPSRreagent08 @21:56:31.54 PTV.dial :puck @21:56:31.55 PBV.dial :waste1 @21:56:31.93 delay 30 seconds @21:57:01.95 PBV.dial between PSV and waste1 @21:57:02.34 PSR.select :air @21:57:02.35 PSV.dial PSR @21:57:03.12 PS.reconfigure :fast @21:57:03.17 PS.seek 2.5ml @21:57:27.10 PSV.dial PTV @21:57:27.99 delay 5 seconds @21:57:33.01 PSV.dial PSR @21:57:33.76 delay 5 seconds @21:57:38.78 PSV.dial PTV @21:57:39.66 delay 5 seconds @21:57:44.68 PSV.dial :air @21:57:45.57 PS.seek 0ml @21:58:08.85 washCycleDA #2 of 2 @21:58:08.86 PSR.select :air @21:58:08.87 PSV.dial PSR @21:58:10.26 Pulling 0.5ml of PSR.wash @21:58:10.27 PSR.select :wash @21:58:10.38 PS.reconfigure :default @21:58:10.42 PS.seek 0.5ml @21:58:16.45 delay 5 seconds @21:58:21.46 PSR.select :air @21:58:21.58 PS.seek 2ml @21:58:37.69 PTV.dial :puck @21:58:37.70 PSV.dial PTV @21:58:38.46 PBV.dial :waste1 @21:58:38.89 PS.seek 1.5ml @21:58:44.78 delay 3 seconds @21:58:47.80 PBV.dial between PSV and waste1 @21:58:48.18 PS.seek 0ml @21:59:04.32 delay 2:00 @22:01:04.34 recoverPSRreagent08 @22:01:04.35 PTV.dial :puck @22:01:04.36 PBV.dial :waste1 @22:01:04.74 delay 30 seconds @22:01:34.76 PBV.dial between PSV and waste1 @22:01:35.13 PSR.select :air @22:01:35.14 PSV.dial PSR @22:01:35.91 PS.reconfigure :fast @22:01:35.96 PS.seek 2.5ml @22:02:00.09 PSV.dial PTV @22:02:00.98 delay 5 seconds @22:02:06.00 PSV.dial PSR @22:02:06.75 delay 5 seconds @22:02:11.77 PSV.dial PTV @22:02:12.53 delay 5 seconds @22:02:17.55 PSV.dial :air @22:02:18.32 PS.seek 0ml @22:02:41.72 getPMLReagent08 @22:02:41.73 PRV.dial :air @22:02:42.19 delay 5 seconds @22:02:47.21 PTV.dial :PRVmixing @22:02:48.53 delay 5 seconds @22:02:53.55 PRV.dial :ab2 @22:02:54.00 PTV.dial :PRVmixing @22:02:54.01 PSV.dial :mixloop @22:02:54.47 PS.reconfigure :default @22:02:54.52 PS.seek 0.3ml @22:02:58.44 delay 2 seconds @22:03:00.45 PRV.dial :air @22:03:00.97 PS.seek 0.55ml @22:03:04.37 addPMLReagent08 @22:03:04.38 PSV.dial :air @22:03:04.85 PS.seek 2ml @22:03:20.53 PSV.dial :PML @22:03:21.00 PTV.dial :mixing @22:03:21.76 PBV.dial :waste1 @22:03:22.18 PS.seek 1.85ml @22:03:24.59 PBV.dial between PSV and waste1 @22:03:24.98 PS.seek 0ml @22:03:44.87 delay 2 seconds @22:03:46.89 delay 8:00 @22:05:44.76 CBV.dial :waste2b @22:05:45.09 CS.reconfigure :default @22:05:45.14 CS.seek 0ml @22:05:48.12 delay 1:00 @22:06:48.14 CTV.dial :air,avoiding: :intake @22:06:49.46 Fix #2 of 2 (with RNAlater) for 10:00 @22:06:49.48 CSV.dial CSR @22:06:49.97 Pulling 2ml of CSR.RNAlater @22:06:50.44 CSR.select :RNAlater @22:06:50.55 CS.seek 2ml @22:06:56.46 delay 5 seconds @22:07:01.48 CSR.select :air @22:07:01.59 CS.reconfigure :fast @22:07:01.64 CS.seek 10ml @22:07:21.52 Pumping wcr puck from tube 5 up to 35psi... @22:07:21.53 CBV.dial SSV @22:07:22.84 CBV.dial between CSV and waste2b @22:07:23.78 CSV.dial between air and CTV @22:07:24.39 CS.seek 7.181ml @22:07:31.71 CTV.dial CSV,avoiding: :air @22:07:31.74 CSV.dial CTV @22:07:33.12 CS.reconfigure :pressurize @22:07:33.17 CS.seek 0ml @22:07:52.08 CSV.dial :air @22:07:52.56 CS.reconfigure :fast @22:07:52.60 CS.seek 10ml @22:08:17.29 CSV.dial between air and CTV @22:08:17.64 CS.seek 4.343ml @22:08:31.43 CTV.dial CSV,avoiding: :air @22:08:31.46 CSV.dial CTV @22:08:31.76 CS.reconfigure :pressurize @22:08:31.81 CS.seek 0ml @22:08:41.48 delay 10:00 @22:11:46.91 recoverPRVreagent08 @22:11:46.92 PTV.dial :mixing @22:11:46.93 PBV.dial :waste1 @22:11:47.30 delay 30 seconds @22:12:17.33 PBV.dial between PSV and waste1 @22:12:17.69 PSV.dial :air @22:12:18.16 PS.reconfigure :fast @22:12:18.21 PS.seek 2.5ml @22:12:42.36 PSV.dial :mixloop @22:12:42.83 delay 2 seconds @22:12:44.85 PSV.dial :air @22:12:45.32 delay 2 seconds @22:12:47.33 PSV.dial :mixloop @22:12:47.80 delay 2 seconds @22:12:49.82 PSV.dial :air @22:12:50.29 PS.seek 0ml @22:13:13.77 washCycleDA #1 of 3 @22:13:13.78 PSR.select :air @22:13:13.79 PSV.dial PSR @22:13:15.18 Pulling 0.5ml of PSR.wash @22:13:15.19 PSR.select :wash @22:13:15.31 PS.reconfigure :default @22:13:15.35 PS.seek 0.5ml @22:13:21.33 delay 5 seconds @22:13:26.35 PSR.select :air @22:13:26.46 PS.seek 2ml @22:13:42.64 PTV.dial :puck @22:13:43.38 PSV.dial PTV @22:13:44.13 PBV.dial :waste1 @22:13:44.56 PS.seek 1.5ml @22:13:50.42 delay 3 seconds @22:13:53.44 PBV.dial between PSV and waste1 @22:13:53.82 PS.seek 0ml @22:14:10.17 delay 2:00 @22:16:10.20 recoverPSRreagent08 @22:16:10.21 PTV.dial :puck @22:16:10.22 PBV.dial :waste1 @22:16:10.59 delay 30 seconds @22:16:40.61 PBV.dial between PSV and waste1 @22:16:40.97 PSR.select :air @22:16:40.98 PSV.dial PSR @22:16:41.75 PS.reconfigure :fast @22:16:41.80 PS.seek 2.5ml @22:17:05.82 PSV.dial PTV @22:17:06.59 delay 5 seconds @22:17:11.61 PSV.dial PSR @22:17:12.37 delay 5 seconds @22:17:17.39 PSV.dial PTV @22:17:18.15 delay 5 seconds @22:17:23.17 PSV.dial :air @22:17:24.06 PS.seek 0ml @22:17:47.61 washCycleDA #2 of 3 @22:17:47.62 PSR.select :air @22:17:47.63 PSV.dial PSR @22:17:49.02 Pulling 0.5ml of PSR.wash @22:17:49.03 PSR.select :wash @22:17:49.14 PS.reconfigure :default @22:17:49.18 PS.seek 0.5ml @22:17:55.08 delay 5 seconds @22:18:00.09 PSR.select :air @22:18:00.21 PS.seek 2ml @22:18:16.29 PTV.dial :puck @22:18:16.30 PSV.dial PTV @22:18:17.18 PBV.dial :waste1 @22:18:17.60 PS.seek 1.5ml @22:18:23.98 delay 3 seconds @22:18:27.00 PBV.dial between PSV and waste1 @22:18:27.38 PS.seek 0ml @22:18:41.49 CBV.dial :waste2b @22:18:41.83 CS.reconfigure :default @22:18:41.87 CS.seek 0ml @22:18:43.54 delay 2:00 @22:18:44.51 delay 1:00 @22:19:44.54 CTV.dial :air,avoiding: :intake @22:20:43.57 recoverPSRreagent08 @22:20:43.58 PTV.dial :puck @22:20:43.59 PBV.dial :waste1 @22:20:43.96 delay 30 seconds @22:21:13.99 PBV.dial between PSV and waste1 @22:21:14.35 PSR.select :air @22:21:14.37 PSV.dial PSR @22:21:15.12 PS.reconfigure :fast @22:21:15.17 PS.seek 2.5ml @22:21:39.07 PSV.dial PTV @22:21:39.84 delay 5 seconds @22:21:44.86 PSV.dial PSR @22:21:45.62 delay 5 seconds @22:21:50.64 PSV.dial PTV @22:21:51.53 delay 5 seconds @22:21:56.55 PSV.dial :air @22:21:57.43 PS.seek 0ml @22:22:20.98 washCycleDA #3 of 3 @22:22:20.99 PSR.select :air @22:22:21.00 PSV.dial PSR @22:22:22.51 Pulling 0.5ml of PSR.wash @22:22:22.52 PSR.select :wash @22:22:22.64 PS.reconfigure :default @22:22:22.68 PS.seek 0.5ml @22:22:28.70 delay 5 seconds @22:22:33.71 PSR.select :air @22:22:33.83 PS.seek 2ml @22:22:49.94 PTV.dial :puck @22:22:49.95 PSV.dial PTV @22:22:50.69 PBV.dial :waste1 @22:22:51.13 PS.seek 1.5ml @22:22:57.04 delay 3 seconds @22:23:00.05 PBV.dial between PSV and waste1 @22:23:00.43 PS.seek 0ml @22:23:16.57 delay 2:00 @22:25:16.60 recoverPSRreagent08 @22:25:16.61 PTV.dial :puck @22:25:16.62 PBV.dial :waste1 @22:25:16.99 delay 30 seconds @22:25:47.01 PBV.dial between PSV and waste1 @22:25:47.38 PSR.select :air @22:25:47.40 PSV.dial PSR @22:25:48.16 PS.reconfigure :fast @22:25:48.21 PS.seek 2.5ml @22:26:12.28 PSV.dial PTV @22:26:13.17 delay 5 seconds @22:26:18.19 PSV.dial PSR @22:26:18.94 delay 5 seconds @22:26:23.96 PSV.dial PTV @22:26:24.72 delay 5 seconds @22:26:29.74 PSV.dial :air @22:26:30.63 PS.seek 0ml @22:26:54.09 Gate.power :camera,:ON @22:26:54.11 Mixing DA Substrates @22:26:54.12 PSR.select :air @22:26:54.13 PSV.dial PSR @22:26:55.71 Pulling 0.25ml of PSR.sub1 @22:26:55.73 PSR.select :sub1 @22:26:55.93 PS.reconfigure :default @22:26:56.06 PS.seek 0.25ml @22:26:59.53 delay 5 seconds @22:27:04.60 Pulling 0.25ml of PSR.sub2 @22:27:04.61 PSR.select :sub2 @22:27:04.73 PS.seek 0.5ml @22:27:08.19 delay 5 seconds @22:27:13.21 PSR.select :air @22:27:13.32 PS.seek 2ml @22:27:29.53 PTV.dial :puck @22:27:29.54 PSV.dial PTV @22:27:30.33 PBV.dial :waste1 @22:27:30.76 PS.seek 1.5ml @22:27:37.03 delay 3 seconds @22:27:40.05 PBV.dial between PSV and waste1 @22:27:40.43 PS.seek 0ml @22:27:56.59 delay 10 seconds @22:28:06.62 recoverPSRreagent08 @22:28:06.63 PTV.dial :puck @22:28:06.64 PBV.dial :waste1 @22:28:07.00 delay 30 seconds @22:28:37.02 PBV.dial between PSV and waste1 @22:28:37.39 PSR.select :air @22:28:37.41 PSV.dial PSR @22:28:38.32 PS.reconfigure :fast @22:28:38.36 PS.seek 2.5ml @22:29:02.81 PSV.dial PTV @22:29:03.58 delay 5 seconds @22:29:08.59 PSV.dial PSR @22:29:09.37 delay 5 seconds @22:29:14.39 PSV.dial PTV @22:29:15.17 delay 5 seconds @22:29:20.19 PSV.dial :air @22:29:20.96 PS.seek 0ml @22:29:45.19 Additional air clear @22:29:45.20 PSR.select :air @22:29:45.21 PSV.dial PSR @22:29:46.61 Pulling 2ml of PSR.air @22:29:46.62 PSR.select :air @22:29:46.64 PS.reconfigure :default @22:29:46.69 PS.seek 2ml @22:30:07.96 delay 5 seconds @22:30:12.98 PTV.dial :puck @22:30:13.00 PSV.dial PTV @22:30:13.78 PBV.dial :waste1 @22:30:14.69 PS.seek 1.5ml @22:30:20.56 delay 3 seconds @22:30:23.58 PBV.dial between PSV and waste1 @22:30:23.95 delay 1 second @22:30:24.97 PSV.dial :waste1 @22:30:26.05 PS.seek 0ml @22:30:42.24 Processing Puck Heater at 27.9C before imaging PH.coast @22:30:42.37 Imaging da puck in Processing Clamp while storing it to tube 4 @22:30:42.53 SC.reconfigure :tight @22:30:42.58 SC.seek 4 @22:30:46.15 SC.configure :limp @22:30:46.34 Elbow.reconfigure :tight @22:30:46.39 Elbow.seek 4 @22:30:48.16 Forearm.reconfigure :tight @22:30:48.21 Forearm.seek 4 + 450 counts @22:30:49.28 SE.reconfigure :pick @22:30:49.33 SE.seek :beamBreak @22:30:52.32 SE.configure :default @22:30:52.46 Elbow.reconfigure :limp @22:30:52.51 Forearm.reconfigure :loose @22:30:52.55 Forearm.seek 4 @22:30:52.78 Elbow.hold @22:30:52.84 Elbow.reconfigure :tight @22:30:52.93 SE.seek 2.18 Pucks below Top Plate @22:30:53.38 Forearm.force -20 @22:30:53.42 Forearm.seek 4 + 286 counts @22:30:53.90 Forearm.reconfigure :tight @22:30:53.94 Forearm.seek 4 + 450 counts @22:30:54.16 SE.seek 3.18 Pucks below Top Plate @22:30:54.66 Forearm.reconfigure :loose @22:30:54.70 Forearm.seek :retract @22:30:55.64 Elbow.seek PC @22:30:58.12 Elbow.reconfigure :loose @22:30:58.21 Forearm.seek PC @22:30:59.17 Hand.close @22:30:59.91 PC.seek :open @22:31:04.68 Forearm.seek :retract @22:31:05.61 Elbow.reconfigure :tight @22:31:05.66 Elbow.seek 4 @22:31:08.24 Forearm.reconfigure :tight @22:31:08.28 Forearm.seek 4 + 450 counts @22:31:09.23 Hand.open @22:31:09.85 SE.seek 2.83 Pucks below Top Plate @22:31:10.12 SE.reconfigure :seal @22:31:10.17 SE.seek 1.86 Pucks below Top Plate @22:31:10.73 Lights, camera, ACTION! @22:31:10.76 PuckCamera: snap -bin=2,2 -auto /var/log/esp/da23apr1522h100ml.tif @22:31:30.21 Waiting >20 seconds for da to relinquish FlushPuck @22:32:52.49 SX HX9/16 (USB:02): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 98.095 seconds /var/log/esp/da23apr1522h100ml.tif: TIFF Upload Complete @22:32:52.52 PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da23apr1522h100ml15s.tif @22:33:08.13 SX HX9/16 (USB:02): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15 seconds /var/log/esp/midres/da23apr1522h100ml15s.tif: TIFF Upload Complete @22:33:08.16 PuckCamera: snap -bin=2,2 20.0 /var/log/esp/midres/da23apr1522h100ml20s.tif @22:33:28.81 SX HX9/16 (USB:02): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 20 seconds /var/log/esp/midres/da23apr1522h100ml20s.tif: TIFF Upload Complete @22:33:28.83 Gate.power :camera,:OFF @22:33:28.84 DAfans.email "98.095 sec auto-exposure",Subject: "da23apr1522h100ml images!" @22:33:29.10 Elbow.seek 4 @22:33:29.28 Forearm.seek 4 + 450 counts @22:33:29.36 SE.reconfigure :gently @22:33:29.41 SE.seek 2.58 Pucks below Top Plate @22:33:30.05 Elbow.reconfigure :limp @22:33:30.12 Forearm.reconfigure :loose @22:33:30.16 Forearm.seek 4 @22:33:30.42 Elbow.hold @22:33:30.49 Elbow.reconfigure :tight @22:33:30.56 SE.reconfigure :default @22:33:30.60 SE.seek 2.77 Pucks below Top Plate @22:33:31.40 Forearm.force -20 @22:33:31.44 Forearm.seek 4 + 265 counts @22:33:31.74 Forearm.reconfigure :tight @22:33:31.79 Forearm.seek 4 + 450 counts @22:33:32.21 SE.seek :down @22:33:33.49 Forearm.reconfigure :loose @22:33:33.59 Forearm.seek :retract @22:33:34.79 SE.stop @22:33:34.99 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @22:33:35.58 Elbow.seek FlushPuck::Garage @22:33:37.54 Elbow.reconfigure :loose @22:33:37.72 Forearm.seek :garage @22:33:38.78 Hand.close @22:33:39.66 Forearm.seek :retract @22:33:40.66 Elbow.reconfigure :tight @22:33:40.74 Elbow.seek PC @22:33:42.24 Elbow.reconfigure :loose @22:33:42.46 Forearm.reconfigure :tight @22:33:42.54 Forearm.seek PC @22:33:44.06 PC.seek :closed @22:33:48.85 Processing Clamp onSpring/offSpring for 14/26 samples, on/off torque 1.55 @22:33:48.99 Hand.open @22:33:49.81 Forearm.reconfigure :loose @22:33:49.89 Forearm.seek :retract @22:33:50.87 PSR.select :air @22:33:50.94 Storing wcr puck in Collection Clamp to tube 4 @22:33:50.96 PSV.dial PSR @22:33:51.40 SC.reconfigure :tight @22:33:51.50 SC.seek 4 @22:33:51.75 Pulling 0.5ml of PSR.flush @22:33:51.77 PSR.select :flush @22:33:51.93 PS.seek 0.5ml @22:33:52.07 SC.configure :limp @22:33:52.48 Elbow.reconfigure :tight @22:33:52.57 Elbow.seek 4 @22:33:55.30 Forearm.reconfigure :tight @22:33:55.38 Forearm.seek 4 + 450 counts @22:33:56.55 SE.reconfigure :pick @22:33:56.65 SE.seek :beamBreak @22:33:57.93 delay 5 seconds @22:33:59.72 SE.configure :default @22:34:00.04 Elbow.reconfigure :limp @22:34:00.14 Forearm.reconfigure :loose @22:34:00.22 Forearm.seek 4 @22:34:00.54 Elbow.hold @22:34:00.68 Elbow.reconfigure :tight @22:34:00.86 SE.seek 3.16 Pucks below Top Plate @22:34:01.51 Forearm.force -20 @22:34:01.59 Forearm.seek 4 + 265 counts @22:34:02.07 Forearm.reconfigure :tight @22:34:02.15 Forearm.seek 4 + 450 counts @22:34:02.44 SE.seek 4.16 Pucks below Top Plate @22:34:03.18 PSR.select :air @22:34:03.32 Forearm.reconfigure :loose @22:34:03.39 Forearm.seek :retract @22:34:03.51 PS.seek 2.5ml @22:34:05.45 Elbow.seek CC @22:34:07.24 Elbow.reconfigure :loose @22:34:07.44 Forearm.seek CC @22:34:08.50 Hand.close @22:34:09.19 CC.seek :open @22:34:12.52 upsync @22:34:13.93 Forearm.seek :retract @22:34:14.95 Elbow.reconfigure :tight @22:34:15.00 Elbow.seek 4 @22:34:16.77 Elbow.reconfigure :limp @22:34:16.82 Forearm.seek 4 @22:34:17.82 Elbow.hold @22:34:17.88 Elbow.reconfigure :tight @22:34:17.93 Hand.open @22:34:18.64 Forearm.force -20 @22:34:18.68 Forearm.seek 4 + 301 counts @22:34:18.98 Forearm.reconfigure :tight @22:34:19.02 Forearm.seek 4 + 450 counts @22:34:19.27 SE.seek :down @22:34:20.40 Forearm.reconfigure :loose @22:34:20.45 Forearm.seek :retract @22:34:21.67 SE.stop @22:34:21.71
Starting finalPurge @22:34:24.77 delay 1 second @22:34:25.79 PRV.dial :waste2,avoiding: :ab2 @22:34:27.65 PTV.dial :PRVmixing @22:34:28.96 PSV.dial :mixloop @22:34:30.00 PS.seek :empty @22:34:41.41
Waiting >20 seconds for psFlush to relinquish FlushPuck @22:34:56.36 PSR.select :air @22:34:56.38 PSV.dial PSR @22:34:57.48 Pulling 0.5ml of PSR.flush @22:34:57.49 PSR.select :flush @22:34:57.61 PS.seek 0.5ml @22:35:03.59 delay 5 seconds @22:35:08.60 PSR.select :air @22:35:08.72 PS.seek 2.5ml @22:35:29.96 delay 1 second @22:35:30.97 PBV.dial :waste1 @22:35:31.32 PTV.dial :mixing @22:35:32.07 PSV.dial :mixloop @22:35:33.12 PS.seek :empty @22:35:59.55 PSV.dial PSR @22:36:00.60 PSR.select :flush @22:36:00.71 PS.seek 1ml @22:36:11.70 PSR.select :air @22:36:11.82 PS.seek 2.5ml @22:36:27.99 PSV.dial PTV @22:36:28.86 PTV.dial :puck @22:36:29.60 PBV.dial :waste1 @22:36:29.62 PS.seek 0ml @22:36:55.99 PSV.dial PSR @22:36:56.72 PSR.select :flush @22:36:56.84 PS.seek 0.5ml @22:37:02.80 PSR.select :air @22:37:02.91 PS.seek 2.5ml @22:37:24.17 PSV.dial PTV @22:37:24.92 PBV.dial PSV @22:37:25.48 PS.seek 0ml @22:37:51.83 PSV.dial PBV @22:37:52.31 PS.seek 2.5ml @22:38:18.70 PSV.dial :waste1 @22:38:19.44 PS.seek 0ml @22:38:45.80 PSV.dial PSR @22:38:46.27 PSR.select :air @22:38:46.29 PS.seek 2.5ml @22:39:12.64 PSV.dial PTV @22:39:13.36 PBV.dial PSV @22:39:13.38 PS.seek 0ml @22:39:39.70 PSV.dial PBV @22:39:40.18 PS.seek 2.5ml @22:40:06.45 PSV.dial :waste1 @22:40:07.18 PS.seek 0ml @22:40:33.54 PSV.dial PSR @22:40:34.01 PSR.select :flush @22:40:34.13 PS.seek 1ml @22:40:45.70 PSR.select :air @22:40:45.81 PS.seek 2.5ml @22:41:02.01 PSV.dial PTV @22:41:02.75 PTV.dial :puck @22:41:02.76 PBV.dial :waste1 @22:41:03.31 PS.seek 0ml @22:41:29.70 PSV.dial PSR @22:41:30.45 PSR.select :flush @22:41:30.57 PS.seek 0.5ml @22:41:36.54 PSR.select :air @22:41:36.66 PS.seek 2.5ml @22:41:57.86 PSV.dial PTV @22:41:58.61 PBV.dial PSV @22:41:59.16 PS.seek 0ml @22:42:25.54 PSV.dial PBV @22:42:26.02 PS.seek 2.5ml @22:42:52.51 PSV.dial :waste1 @22:42:53.25 PS.seek 0ml @22:43:19.63 PSV.dial PSR @22:43:20.10 PSR.select :air @22:43:20.13 PS.seek 2.5ml @22:43:46.67 PSV.dial PTV @22:43:47.40 PBV.dial PSV @22:43:47.42 PS.seek 0ml @22:44:13.73 PSV.dial PBV @22:44:14.20 PS.seek 2.5ml @22:44:40.57 PSV.dial :waste1 @22:44:41.31 PS.seek 0ml @22:45:07.64
Loading FlushPuck into Collection Clamp from Processing Clamp @22:45:07.81 Elbow.seek PC @22:45:10.29 Elbow.reconfigure :loose @22:45:10.38 Forearm.seek PC @22:45:11.36 Hand.close @22:45:12.02 PC.seek :open @22:45:16.71 Forearm.seek :retract @22:45:17.68 Elbow.reconfigure :tight @22:45:17.72 Elbow.seek CC @22:45:21.26 Elbow.reconfigure :loose @22:45:21.35 Forearm.reconfigure :tight @22:45:21.39 Forearm.seek CC @22:45:22.62 CC.seek :closed @22:45:27.18 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.35 @22:45:27.25 Hand.open @22:45:27.95 Forearm.reconfigure :loose @22:45:28.00 Forearm.seek :retract @22:45:28.96 Flushing the Collection Valve::Manifold @22:45:29.08 CSV.dial CSR @22:45:29.57 Pulling 5ml of CSR.flush CSR.select :flush @22:45:29.69 CS.seek 5ml @22:45:43.51 delay 5 seconds @22:45:48.53 CSR.select :air @22:45:48.68 CS.reconfigure :fast @22:45:48.72 CS.seek 7.501ml @22:45:55.48 CSV.dial :waste @22:45:56.76 CS.reconfigure :default @22:45:56.81 CS.seek 0ml @22:46:16.76 Flushing lower manifold's waste1 port @22:46:16.88 CSV.dial :flush @22:46:17.31 CS.reconfigure :fast @22:46:17.35 CS.seek 5ml @22:46:30.07 delay 5 seconds @22:46:35.09 CSV.dial :air @22:46:35.61 CS.seek 7.001ml @22:46:41.10 CSV.dial CTV @22:46:41.13
CTV.dial CSV @22:46:42.52 CBV.dial :waste1 @22:46:43.28 CS.seek 0ml @22:46:59.98 Flushing lower manifold's waste2 port @22:47:00.10 CSV.dial :flush @22:47:00.81 CS.seek 5ml @22:47:13.63 delay 5 seconds @22:47:18.65 CSV.dial :air @22:47:19.16 CS.seek 6.999ml @22:47:25.32 CSV.dial CTV @22:47:25.35
CTV.dial CSV @22:47:25.79 CBV.dial :waste2 @22:47:26.84 CS.seek 0ml @22:47:43.57 Flushing lower manifold's waste2b port @22:47:43.69 CSV.dial :flush @22:47:44.42 CS.seek 5ml @22:47:57.17 delay 5 seconds @22:48:02.18 CSV.dial :air @22:48:02.70 CS.seek 7ml @22:48:08.20 CSV.dial CTV @22:48:08.23
CTV.dial CSV @22:48:08.67 CBV.dial :waste2b @22:48:09.72 CS.seek 0ml @22:48:26.42 Disinfecting external Intake with kill @22:48:26.54 CSV.dial CSR @22:48:27.14 Pulling 8ml of CSR.kill @22:48:27.15 CSR.select :kill @22:48:27.26 CS.seek 8ml @22:48:47.14 delay 5 seconds @22:48:52.15 CSR.select :air @22:48:52.27 CS.seek 10ml @22:48:57.79 CSV.dial CBV @22:48:57.82
CBV.dial CSV @22:48:59.01 CTV.dial :intake @22:48:59.80 Intake.select :open @22:48:59.91 CS.seek 2ml @22:49:18.88 Intake.select :close @22:49:18.98 CTV.dial :air @22:49:19.73 CS.reconfigure :default @22:49:19.77 CS.seek 0ml @22:49:25.69 CSV.dial CSR @22:49:26.86 Pulling 5ml of CSR.flush @22:49:26.87 CSR.select :flush @22:49:26.98 CS.seek 5ml @22:49:40.57 delay 5 seconds @22:49:45.59 CSR.select :air @22:49:45.70 CS.seek 10ml @22:49:59.29 CSV.dial CBV @22:50:00.28 CS.seek 0ml @22:50:26.63 Garaging FlushPuck in Collection Clamp @22:50:26.70 Elbow.reconfigure :tight @22:50:26.74 Elbow.seek CC @22:50:26.86 Elbow.reconfigure :loose @22:50:26.94 Forearm.seek CC @22:50:27.90 Hand.close @22:50:28.52 CC.seek :open @22:50:33.11 Forearm.seek :retract @22:50:34.06 Elbow.reconfigure :tight @22:50:34.11 Elbow.seek :garage @22:50:37.04 Elbow.reconfigure :loose @22:50:37.13 Forearm.reconfigure :tight @22:50:37.17 Forearm.seek :garage @22:50:38.17 Hand.open @22:50:38.83 Forearm.reconfigure :loose @22:50:38.87 Forearm.seek :retract @22:50:39.88 Completed [initialPurge; da [100, 100] #da and wsr are run simultanelously] begun 20:57:22 @22:50:39.92 Asleep after completing DA_WCR_041523 assays @22:50:39.94 GoodNews.email "{1 => 33,\n 2 => 33,\n 3 => 33,\n 4 => 5,\n 5 => 28,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@22:46:32, 17% flow, 22.3C, 51% humidity, 15.0psia, 13.539V, 0.460A, 0.441A avg, 6.22W\nSampled 100.0ml",Subject: "Asleep after completing DA_WCR_041523 assays"