+ ESPwhich DA_WCR_91823.rb + fn=DA_WCR_91823.rb + IFS=: + set /home/esp/esp2/mission . + fullPath=/home/esp/esp2/mission/DA_WCR_91823.rb + [ -f /home/esp/esp2/mission/DA_WCR_91823.rb ] + realpath /home/esp/esp2/mission/DA_WCR_91823.rb + return 0 + scriptPath=/home/esp/esp2local/mission/DA_WCR_91823.rb + exec ruby /home/esp/esp2/lib/esp.rb /home/esp/esp2local/mission/DA_WCR_91823.rb @21:38:10.52UTC18-Sep-23
ESP2 v3.42 I2C Gateway v3.20 for core bus @21:38:13.20 Configuration for short puck jake 1/11/21 by brent@mbari.org /home/esp/esp2/type/shallow/jake/configure.rb @21:38:17.37 Polling Can every 10:00 @21:38:17.38 Cleanly exited at 21:37:49UTC18-Sep-23 @21:38:17.43 Loading /home/esp/esp2local/mission/DA_WCR_91823.rb mission @21:38:17.48 GoodNews.email "Starting DA_WCR_91823 w/pucks in tube 2\n{}\nCTD data unavailable\nCan@21:38:17, 25% flow, 20.4C, 23% humidity, 14.8psia, 13.769V, 0.242A, 0.028Ah, 3.33W",Subject: "DA_WCR_91823 begun" @21:38:17.51
Preparing to sleep until + 00:00:05 @21:38:17.56 Asleep til + 00:00:05 @21:38:17.58 Continuing at 21:38 @21:38:17.86 Gate.power :core,:OFF @21:38:17.88 Gate.power :raw,:OFF Shutdown @21:38:20.49 GoodNews.email "Then, in tube 2: initialPurge; da [50, 50] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_91823.rb:14\n{1 => 0,\n 2 => 33,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@21:38:17, 25% flow, 20.4C, 23% humidity, 14.8psia, 13.769V, 0.242A, 0.028Ah, 3.33W",Subject: "Asleep until 21:38" @21:38:22.04 upsync @21:38:22.50
Powering core bus @21:38:24.53 upsync @21:38:27.95
Gate.power :raw,:ON @21:38:28.92 CS.configure :default @21:38:28.97 PS.configure :default @21:38:29.02 SS.configure :default @21:38:29.07 CC.configure :default @21:38:29.12 PC.configure :default @21:38:29.19 Forearm.configure :loose @21:38:29.23 Elbow.configure :loose @21:38:29.30 SC.configure :loose @21:38:29.35 SE.configure :default @21:38:29.40 CH.configure :default @21:38:29.43 PH.configure :default @21:38:29.47 All dwarf controllers running firmware version 3.85 @21:38:29.58 Valve.reset @21:38:30.22 Intake.select :close @21:38:30.26 Exhaust.select :close @21:38:30.28 CTV.dial :air @21:38:30.39 PSV.dial :air @21:38:30.43 CSV.dial :waste1 @21:38:30.56 PS.home.seek 0ml @21:38:30.59
Forearm.home.seek :retract @21:38:30.93 SSV.dial :spit,avoiding: :exhaust @21:38:31.11 CS.reconfigure :fast @21:38:31.15 SS.home.seek 0ml @21:38:31.23 CS.home.seek 0ml @21:38:31.36 Hand.close @21:38:31.40
Elbow.reconfigure :loose @21:38:31.49 Elbow.jog 1500 @21:38:31.89 Elbow.reconfigure :loose @21:38:31.94 Elbow.home.seek :clear @21:38:35.15 SE.home.seek :down @21:38:35.76 SC.reconfigure :tight @21:38:35.81 SC.home.seek :initial @21:38:38.84 PS.stop @21:38:54.76
SC.configure :limp @21:38:54.81 Forearm.seek :retract @21:38:54.88 Elbow.seek :hammer @21:38:55.90 Forearm.reconfigure :tight @21:38:55.95 Forearm.seek retracted @21:38:56.33 PC.home.seek :open @21:38:56.35
CC.home.seek :open @21:38:59.87 Phase [initialPurge; da [50, 50] #da and wsr are run simultanelously] @21:38:59.88 Notice.email "At 21:38:59 began in tube 2: initialPurge; da [50, 50] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_91823.rb:14\n{1 => 0,\n 2 => 33,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@21:38:59, 26% flow, 20.3C, 23% humidity, 14.8psia, 13.710V, 0.272A, 0.032Ah, 3.73W",Subject: "Started DA_WCR_91823 phase initialPurge; da [50, 50] #da and wsr are run simultanelously" @21:38:59.90
Starting initialPurge @21:38:59.93 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @21:38:59.99 Hand.open @21:39:00.69 Elbow.reconfigure :tight @21:39:00.74 Elbow.seek FlushPuck::Garage @21:39:02.65 Elbow.reconfigure :loose @21:39:02.88 Forearm.reconfigure :loose @21:39:02.98 Forearm.seek :garage @21:39:03.97 Hand.close @21:39:04.21 upsync @21:39:04.69
Forearm.seek :retract @21:39:05.71 Elbow.reconfigure :tight @21:39:05.75 Elbow.seek CC @21:39:08.70 Elbow.reconfigure :loose @21:39:08.80 Forearm.reconfigure :tight @21:39:08.85 Forearm.seek CC @21:39:10.07 CC.seek :closed @21:39:14.58 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.43 @21:39:14.64 Hand.open @21:39:15.37 Forearm.reconfigure :loose @21:39:15.42 Forearm.seek :retract @21:39:16.38 Flushing the Collection Valve::Manifold @21:39:16.50 CSV.dial CSR @21:39:17.78 Pulling 5ml of CSR.flush @21:39:17.79 CSR.select :flush @21:39:17.90 CS.reconfigure :default @21:39:17.95 CS.seek 5ml @21:39:31.95 delay 5 seconds @21:39:36.97 CSR.select :air @21:39:37.14 CS.reconfigure :fast @21:39:37.18 CS.seek 7.5ml @21:39:43.91 CSV.dial :waste @21:39:45.20 CS.reconfigure :default @21:39:45.25 CS.seek 0ml @21:40:05.26 Purging external Intake @21:40:05.38 CSV.dial :flush @21:40:05.83 CS.reconfigure :fast @21:40:05.87 CS.seek 10ml @21:40:30.35 delay 5 seconds @21:40:35.37 CSV.dial CBV @21:40:35.41
CBV.dial CSV @21:40:36.16 CTV.dial :intake @21:40:36.96 Intake.select :open @21:40:37.07 CS.seek 0ml @21:41:00.54 Intake.select :close @21:41:00.64 CTV.dial :air @21:41:01.43 Flushing upper manifold @21:41:01.55 CSV.dial :flush @21:41:02.30 CS.seek 5ml @21:41:15.20 delay 5 seconds @21:41:20.21 CSV.dial :air @21:41:20.85 CS.seek 7.001ml @21:41:26.35 CSV.dial CBV @21:41:26.38
CBV.dial CSV @21:41:27.38 CTV.dial :air @21:41:27.47 CS.seek 0ml @21:41:44.16 delay 7 seconds @21:41:51.19 CS.seek 3ml @21:41:59.29 CSV.dial :waste @21:41:59.75 CS.seek 0ml @21:42:07.65 CSV.dial :flush @21:42:08.09 CS.seek 3ml @21:42:16.07 delay 5 seconds @21:42:21.09 CSV.dial :air @21:42:21.71 CS.seek 5ml @21:42:27.35 CSV.dial :air @21:42:27.43 CS.seek 0ml @21:42:39.57 delay 7 seconds @21:42:46.60 CS.seek 3ml @21:42:54.60 CSV.dial :waste @21:42:55.32 CS.seek 0ml @21:43:03.04 Garaging FlushPuck in Collection Clamp @21:43:03.11 Elbow.reconfigure :tight @21:43:03.15 Elbow.seek CC @21:43:03.41 Elbow.reconfigure :loose @21:43:03.51 Forearm.seek CC @21:43:04.48 Hand.close @21:43:05.06 CC.seek :open @21:43:10.12 Forearm.seek :retract @21:43:11.12 Elbow.reconfigure :tight @21:43:11.17 Elbow.seek :garage @21:43:14.06 Elbow.reconfigure :loose @21:43:14.16 Forearm.reconfigure :tight @21:43:14.21 Forearm.seek :garage @21:43:15.24 Hand.open @21:43:15.86 Forearm.reconfigure :loose @21:43:15.91 Forearm.seek :retract @21:43:16.89 da sampling at most 50ml, wcr at most 50ml @21:43:16.92 Loading sh1 puck from tube 2 into Collection Clamp @21:43:16.94 DAfans.email "Started da in tube 2 at 21:43:16UTC18-Sep-23\n{1 => 0,\n 2 => 33,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@21:38:59, 26% flow, 20.3C, 23% humidity, 14.8psia, 13.710V, 0.272A, 0.032Ah, 3.73W",Subject: "da sampling at most 50ml, wcr at most 50ml" @21:43:17.22
SC.reconfigure :tight @21:43:17.28 SC.seek 2 @21:43:20.88 upsync @21:43:24.05
SC.configure :limp @21:43:24.29 Elbow.reconfigure :tight @21:43:24.34 Elbow.seek 2 @21:43:26.24 Forearm.reconfigure :tight @21:43:26.29 Forearm.seek 2 + 450 counts @21:43:27.32 SE.reconfigure :pick @21:43:27.38 SE.seek :beamBreak @21:43:28.36 SE.configure :default @21:43:28.52 Elbow.seek 2 @21:43:28.71 Forearm.seek 2 + 300 counts @21:43:29.00 Forearm.reconfigure :loose @21:43:29.05 Hand.close @21:43:29.70 SE.seek :down @21:43:29.90
Forearm.seek :retract @21:43:30.40 SE.stop @21:43:31.27
Elbow.seek CC @21:43:32.86 Elbow.reconfigure :loose @21:43:32.96 Forearm.reconfigure :tight @21:43:33.01 Forearm.seek CC @21:43:34.28 CC.seek :closed @21:43:38.80 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.25 @21:43:38.81 Hand.open @21:43:39.49 Forearm.reconfigure :loose @21:43:39.54 Forearm.seek :retract @21:43:40.56 collectSample 50ml w/bubble point of 24psi @21:43:40.57 Priming 7ml @21:43:40.58 Intake.select :close @21:43:40.59 Exhaust.select :close @21:43:40.60 SS.hold @21:43:40.81 Calibrating pressure and temperature sensors @21:43:40.84 CTV.dial :air @21:43:40.91 SSV.dial :spit,avoiding: :exhaust @21:43:40.99 SS.reconfigure :fast @21:43:41.04 SS.seek 5ml @21:43:43.88 SSV.dial CBV @21:43:43.91
CBV.dial SSV @21:43:45.24 delay 3.8 seconds @21:43:49.16 Sensor limits: Intake max 89.7psia; Outlet max 85.7psia @21:43:49.17 SSV.dial :spit @21:43:50.49 SS.seek 0ml @21:43:53.47 SSV.dial CBV @21:43:54.78 CTV.dial :intake @21:43:55.59 SSV.dial CBV @21:43:55.61
CBV.dial SSV @21:43:55.85 Allowing 40s for 7.0ml of remaining 7.0ml @21:43:55.86 Intake.select :open @21:43:58.02 Intake @14.4psia, vacuum from 8.9 to 11.9psi @21:43:58.04 SS.reconfigure :inhaling 2.20ml/s @21:43:58.09 SS.seek 7ml @21:44:03.90 Stroke completed -- waiting for pressure to equalize ... @21:44:03.91 SS.reconfigure :exhale @21:44:03.96 SS.hold @21:44:07.02 Intake.select :close @21:44:07.19 SSV.dial :exhaust @21:44:07.95 Exhaust.select :open @21:44:08.07 SS.seek 0ml @21:44:11.97 Exhaust.select :close @21:44:12.08 Sampling 50.0ml @21:44:12.09 Intake.select :close @21:44:12.10 Exhaust.select :close @21:44:12.12 SS.reconfigure :default @21:44:12.16 SS.hold @21:44:12.36 CTV.dial :intake @21:44:12.54 SSV.dial CBV @21:44:12.57
CBV.dial SSV @21:44:13.34 Allowing 130s for 25.0ml of remaining 50.0ml @21:44:13.36 Intake.select :open @21:44:15.52 Intake @14.6psia, vacuum from 9.1 to 12.1psi @21:44:15.54 SS.reconfigure :inhaling 2.20ml/s @21:44:15.58 SS.seek 25ml @21:44:30.95 Stroke completed -- waiting for pressure to equalize ... @21:44:30.96 SS.reconfigure :exhale @21:44:31.01 SS.hold @21:44:34.53 Intake.select :close @21:44:34.68 SSV.dial :exhaust @21:44:35.42 Exhaust.select :open @21:44:35.53 SS.seek 0ml @21:44:44.22 Exhaust.select :close @21:44:44.33 SSV.dial CBV @21:44:44.35
CBV.dial SSV @21:44:45.13 Allowing 130s for 25.0ml of remaining 25.0ml @21:44:45.14 Intake.select :open @21:44:47.30 Intake @14.7psia, vacuum from 9.2 to 12.2psi @21:44:47.32 SS.reconfigure :inhaling 2.20ml/s @21:44:47.36 SS.seek 25ml @21:45:02.59 Stroke completed -- waiting for pressure to equalize ... @21:45:02.60 SS.reconfigure :exhale @21:45:02.65 SS.hold @21:45:06.59 Intake.select :close @21:45:06.74 SSV.dial :exhaust @21:45:07.50 Exhaust.select :open @21:45:07.61 SS.seek 0ml @21:45:16.64 Exhaust.select :close @21:45:16.75 Sampled 50.0ml @21:45:16.76 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @21:45:16.77 Intake.select :close @21:45:16.84 CSV.dial :waste @21:45:16.97 SSV.dial CBV,avoiding: :exhaust @21:45:17.00
CBV.dial SSV @21:45:17.12 CS.seek 0ml @21:45:17.21 CSV.dial :air @21:45:17.95 CS.seek 10ml @21:45:18.88
CTV.dial :air @21:45:19.66 delay 15 seconds @21:45:34.68 SSV.dial :spit,avoiding: :exhaust @21:45:35.99 CTV.dial CSV,avoiding: :intake @21:45:36.03
SS.seek 0ml @21:45:36.15 SSV.dial CBV,avoiding: :exhaust @21:45:42.35 SSV.dial between CBV and plug @21:45:42.85 CBV.dial between SSV and waste2 @21:45:43.25 CTV.dial CSV,avoiding: :air @21:45:43.28
CSV.dial CTV @21:45:43.76 CS.reconfigure :pressurize @21:45:43.81 CS.seek 0ml @21:46:01.51 BubblePoint: Intake @24.2psia, Outlet @0.6psia @21:46:01.52 CBV.dial SSV @21:46:01.85 SS.reconfigure :default @21:46:01.90 SS.seek 10ml @21:46:07.75 delay 15 seconds @21:46:22.88 Evacuated: Intake @23.8psia, Outlet @23.6psia @21:46:22.89 SSV.dial :spit,avoiding: :exhaust @21:46:23.91 SS.reconfigure :exhale @21:46:23.96 SS.seek 0ml @21:46:29.07 SS.reconfigure :fast @21:46:29.12 SS.seek 5ml @21:46:32.14 SSV.dial CBV,avoiding: :exhaust @21:46:32.17
CBV.dial SSV @21:46:33.52 CTV.dial :air,avoiding: :intake @21:46:34.89 CSV.dial CTV @21:46:34.94
SS.seek 10ml @21:46:36.04 CSV.dial :air @21:46:36.49 CS.reconfigure :fast @21:46:36.54 CS.seek 0ml @21:46:37.81
SSV.dial :spit,avoiding: :exhaust @21:46:39.13 SS.reconfigure :exhale @21:46:39.18 SS.seek 0ml @21:46:44.20 SS.reconfigure :fast @21:46:44.24 SS.seek 5ml @21:46:44.91 CS.stop @21:46:47.11 SSV.dial CBV,avoiding: :exhaust @21:46:47.14
CBV.dial SSV @21:46:49.48 SSV.dial :spit,avoiding: :exhaust @21:46:50.77 SS.reconfigure :default @21:46:50.82 SS.seek 0ml @21:46:54.09 SS.stop @21:46:54.13 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @21:46:54.14 Intake.select :close @21:46:54.21 CSV.dial :waste @21:46:54.33 SSV.dial CBV,avoiding: :exhaust @21:46:54.36
CBV.dial SSV @21:46:54.97 CS.seek 0ml @21:46:55.03 CSV.dial :air @21:46:55.69
CTV.dial :air @21:46:55.77 CS.seek 10ml @21:46:55.92
delay 15 seconds @21:47:10.94 SSV.dial :spit,avoiding: :exhaust @21:47:12.24 CTV.dial CSV,avoiding: :intake @21:47:12.28
SS.reconfigure :exhale @21:47:12.38 SS.seek 0ml @21:47:12.76 SSV.dial CBV,avoiding: :exhaust @21:47:20.13 SSV.dial between CBV and plug @21:47:20.61 CBV.dial between SSV and waste2 @21:47:20.99 CSV.dial between air and CTV @21:47:21.32 CS.seek 8.356ml @21:47:25.91 CTV.dial CSV,avoiding: :air @21:47:25.94
CSV.dial CTV @21:47:26.32 CS.reconfigure :pressurize @21:47:26.37 CS.seek 0ml @21:47:43.28 BubblePoint: Intake @24.2psia, Outlet @0.3psia @21:47:43.29 CBV.dial SSV @21:47:43.61 SS.reconfigure :default @21:47:43.66 SS.seek 10ml @21:47:49.32 delay 15 seconds @21:48:04.45 Evacuated: Intake @23.8psia, Outlet @12.6psia @21:48:04.46 SSV.dial :spit,avoiding: :exhaust @21:48:05.50 SS.reconfigure :exhale @21:48:05.55 SS.seek 0ml @21:48:10.52 SS.reconfigure :fast @21:48:11.00 SS.seek 5ml @21:48:13.84 SSV.dial CBV,avoiding: :exhaust @21:48:13.87
CBV.dial SSV @21:48:15.23 CTV.dial :air,avoiding: :intake @21:48:16.55 CSV.dial CTV @21:48:16.60
SS.seek 10ml @21:48:17.77 CSV.dial :air @21:48:18.21 CS.reconfigure :fast @21:48:18.26 CS.seek 0ml @21:48:19.09
SSV.dial :spit,avoiding: :exhaust @21:48:20.41 SS.reconfigure :exhale @21:48:20.46 SS.seek 0ml @21:48:23.56 CS.stop @21:48:25.86
SS.reconfigure :fast @21:48:25.91 SS.seek 5ml @21:48:28.69 SSV.dial CBV,avoiding: :exhaust @21:48:28.72
CBV.dial SSV @21:48:31.07 SSV.dial :spit,avoiding: :exhaust @21:48:32.37 SS.reconfigure :default @21:48:32.42 SS.seek 0ml @21:48:35.76 SS.stop @21:48:35.80 Lysing: Adding 2.0ml AqMeOH @21:48:35.81 CBV.dial between CSV and waste1,avoiding: :DBV @21:48:37.28 CTV.dial CSV @21:48:38.61 CSV.dial CSR @21:48:39.40 Pulling 1ml of CSR.AqMeOH @21:48:39.41 CSR.select :AqMeOH @21:48:39.53 CS.reconfigure :default @21:48:39.57 CS.seek 1ml @21:48:43.22 delay 5 seconds @21:48:48.24 CSR.select :air @21:48:48.41 CS.seek 6ml @21:49:02.09 delay 2 seconds @21:49:04.11 CSV.dial :waste2 @21:49:04.68 CS.reconfigure :fast @21:49:04.72 CS.seek 0ml @21:49:19.18 CSV.dial CSR @21:49:19.63 Pulling 2ml of CSR.AqMeOH CSR.select :AqMeOH @21:49:19.75 CS.reconfigure :default @21:49:19.79 CS.seek 2ml @21:49:25.81 delay 5 seconds @21:49:30.83 CSR.select :air @21:49:31.00 CS.seek 5.001ml @21:49:39.47 delay 2 seconds @21:49:41.48 CSV.dial CTV @21:49:41.93 CS.seek 1ml @21:49:53.26 Before lysing Collection Puck Heater at 20C/25.2psia @21:49:53.28 Heating Puck.from 2, :sh1 in Collection Clamp to 70C for 10:00 @21:49:53.30 CH.seek 70C,8:00 @21:51:52.55 CH.hold 10:00,70C @21:51:52.74 Begun lysing Collection Puck Heater at 68.1C/30.8psia @22:01:52.75 Ended lysing Collection Puck Heater at 70C/29.3psia @22:01:52.77 CS.seek 0ml @22:01:56.40 CTV.dial between spare and air,avoiding: :intake @22:01:57.44 CSV.dial :air @22:01:57.89 CS.seek 5ml @22:02:11.48 CSV.dial CTV @22:02:12.38 delay 2 seconds @22:02:14.39 CSV.dial :air @22:02:14.84 delay 2 seconds @22:02:16.86 CSV.dial CTV @22:02:17.34 delay 2 seconds @22:02:19.36 CSV.dial :air @22:02:19.81 delay 2 seconds @22:02:21.84 CS.seek 0ml @22:02:35.54 Puck @59.8psia after final lysis pressurization @22:02:35.55 Recovering Lysate into Collection syringe @22:02:35.56 CSV.dial CBV @22:02:36.58 CBV.dial CSV @22:02:36.95 CS.seek 5ml @22:02:50.64 delay 5 seconds @22:02:55.66 CTV.dial :air @22:02:56.20 CS.seek 7.001ml @22:03:02.08 delay 5 seconds @22:03:07.09 CSV.dial :air2 @22:03:07.54 CS.seek 10ml @22:03:16.08 CSV.dial between air and CTV,avoiding: CBV @22:03:17.24 Lysing Complete @22:03:17.30 Storing sh1 puck in Collection Clamp to tube 1 @22:03:17.48 SC.reconfigure :tight @22:03:17.53 SC.seek 1 @22:03:21.09 SC.configure :limp @22:03:21.25 Elbow.reconfigure :tight @22:03:21.29 SE.reconfigure :place @22:03:21.37
Elbow.seek CC @22:03:21.45 SE.seek :beamBreak @22:03:21.72
Elbow.reconfigure :loose @22:03:21.82 Forearm.seek CC @22:03:22.91 Hand.close @22:03:23.54 CC.seek :open @22:03:24.26 SE.configure :default @22:03:24.32 SE.seek :place @22:03:28.28
Forearm.seek :retract @22:03:29.26 Elbow.reconfigure :tight @22:03:29.31 Elbow.seek 1 @22:03:30.95 Elbow.reconfigure :limp @22:03:31.00 Forearm.seek 1 @22:03:31.97 Elbow.hold @22:03:32.04 Elbow.reconfigure :tight @22:03:32.09 Hand.open @22:03:33.32 Forearm.force -20 @22:03:33.37 Forearm.seek 1 + 301 counts @22:03:33.67 Forearm.reconfigure :tight @22:03:33.72 Forearm.seek 1 + 450 counts @22:03:33.99 SE.seek :down @22:03:35.13
Forearm.reconfigure :loose @22:03:35.17 Forearm.seek :retract @22:03:36.68 SE.stop @22:03:36.72
Tranferring DA @22:03:36.73 Beginning samplePassC @22:03:36.75 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @22:03:36.94 Elbow.seek FlushPuck::Garage @22:03:38.77 Elbow.reconfigure :loose @22:03:38.87 Forearm.seek :garage @22:03:39.82 Hand.close @22:03:40.53 Forearm.seek :retract @22:03:41.52 Elbow.reconfigure :tight @22:03:41.57 Elbow.seek CC @22:03:44.47 Elbow.reconfigure :loose @22:03:44.57 Forearm.reconfigure :tight @22:03:44.62 Forearm.seek CC @22:03:45.90 CC.seek :closed @22:03:50.43 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.44 @22:03:50.49 Hand.open @22:03:51.24 Forearm.reconfigure :loose @22:03:51.29 Forearm.seek :retract @22:03:52.24 PSV.dial PSR @22:03:53.75 Pulling 1.5ml of PSR.flush @22:03:53.76 PSR.select :flush @22:03:53.88 PS.seek 1.5ml @22:04:10.03 delay 5 seconds @22:04:15.04 PSV.dial :waste1 @22:04:15.53 PS.seek 0ml @22:04:31.90 PSV.dial PSR @22:04:32.37 PSR.select :air @22:04:32.49 PS.seek 2.5ml @22:04:58.87 PRV.dial CBV,avoiding: :ab2 @22:04:58.90
CBV.dial PRV @22:04:59.68 PSV.dial :mixloop @22:05:00.71 CTV.dial :air,avoiding: :intake @22:05:00.79 PTV.dial :PRVmixing @22:05:02.11 PS.seek 0ml @22:05:28.43 PSV.dial :air @22:05:29.01 PS.seek 2.5ml @22:05:55.40 PSV.dial :mixloop @22:05:55.86 PS.seek 0ml @22:06:22.18 PSV.dial :air @22:06:22.65 PS.seek 2.5ml @22:06:48.96 PSV.dial :mixloop @22:06:49.43 PS.seek 0ml @22:07:15.84 Garaging FlushPuck in Collection Clamp @22:07:15.90 Elbow.reconfigure :tight @22:07:15.95 Elbow.seek CC @22:07:16.23 Elbow.reconfigure :loose @22:07:16.33 Forearm.seek CC @22:07:17.36 Hand.close @22:07:17.91 CC.seek :open @22:07:22.53 Forearm.seek :retract @22:07:23.52 Elbow.reconfigure :tight @22:07:23.57 Elbow.seek :garage @22:07:26.48 Elbow.reconfigure :loose @22:07:26.58 Forearm.reconfigure :tight @22:07:26.63 Forearm.seek :garage @22:07:27.57 Hand.open @22:07:28.29 Forearm.reconfigure :loose @22:07:28.33 Forearm.seek :retract @22:07:29.30 PSV.dial :mixloop @22:07:29.38 PTV.dial :PRVmixing @22:07:29.46 PRV.dial CBV @22:07:29.50
CBV.dial PRV @22:07:29.62 CTV.dial :air,avoiding: :intake @22:07:29.70 Loading lyfil puck from tube 2 into Collection Clamp @22:07:29.94 SC.reconfigure :tight @22:07:29.98 SC.seek 2 @22:07:33.56 SC.configure :limp @22:07:33.77 Elbow.reconfigure :tight @22:07:33.82 Elbow.seek 2 @22:07:35.72 Forearm.reconfigure :tight @22:07:35.77 Forearm.seek 2 + 450 counts @22:07:36.81 SE.reconfigure :pick @22:07:36.87 SE.seek :beamBreak @22:07:37.86 SE.configure :default @22:07:38.02 Elbow.seek 2 @22:07:38.21 Forearm.seek 2 + 300 counts @22:07:38.50 Forearm.reconfigure :loose @22:07:38.55 Hand.close @22:07:39.17 SE.seek :down @22:07:39.38
Forearm.seek :retract @22:07:39.84 SE.stop @22:07:40.33
Elbow.seek CC @22:07:41.93 Elbow.reconfigure :loose @22:07:42.03 Forearm.reconfigure :tight @22:07:42.48 Forearm.seek CC @22:07:43.84 CC.seek :closed @22:07:48.36 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.21 @22:07:48.37 Hand.open @22:07:49.11 Forearm.reconfigure :loose @22:07:49.15 Forearm.seek :retract @22:07:50.13 CTV.dial CSV,avoiding: :intake @22:07:51.51 CSV.dial CTV @22:07:51.84 PS.seek 2.5ml @22:08:18.21 CS.seek 4.5ml @22:08:33.10 delay 8 seconds @22:08:41.12 PSV.dial :air @22:08:41.58 delay 2 seconds @22:08:43.60 PSV.dial :waste2 @22:08:44.34 PS.seek 0ml @22:09:10.74 CBV.dial between PRV and waste1 @22:09:11.07 CSV.dial :air @22:09:11.53 CS.seek 10ml @22:09:26.51 CSV.dial CTV @22:09:26.97 CS.seek 0ml @22:09:53.35 CBV.dial :waste1 @22:09:53.66 delay 5 seconds @22:09:58.69 CTV.dial :air,avoiding: :intake @22:10:00.10 Storing lyfil puck in Collection Clamp to tube 1 @22:10:00.27 SC.reconfigure :tight @22:10:00.32 SC.seek 1 @22:10:03.88 SC.configure :limp @22:10:04.47 Elbow.reconfigure :tight @22:10:04.59 Elbow.seek 1 @22:10:06.36 Forearm.reconfigure :tight @22:10:06.41 Forearm.seek 1 + 450 counts @22:10:07.50 SE.reconfigure :pick @22:10:07.55 SE.seek :beamBreak @22:10:10.61 SE.configure :default @22:10:10.77 Elbow.reconfigure :limp @22:10:10.82 Forearm.reconfigure :loose @22:10:10.86 Forearm.seek 1 @22:10:11.03 Elbow.hold @22:10:11.09 Elbow.reconfigure :tight @22:10:11.19 SE.seek 0.16 Pucks below Top Plate @22:10:11.62 Forearm.force -20 @22:10:11.66 Forearm.seek 1 + 317 counts @22:10:11.96 Forearm.reconfigure :tight @22:10:12.01 Forearm.seek 1 + 450 counts @22:10:12.21 SE.seek 1.16 Pucks below Top Plate @22:10:12.68 Forearm.reconfigure :loose @22:10:12.72 Forearm.seek :retract @22:10:13.67 Elbow.seek CC @22:10:15.37 Elbow.reconfigure :loose @22:10:15.47 Forearm.seek CC @22:10:16.47 Hand.close @22:10:17.03 CC.seek :open @22:10:21.78 Forearm.seek :retract @22:10:22.81 Elbow.reconfigure :tight @22:10:22.86 Elbow.seek 1 @22:10:25.04 Elbow.reconfigure :limp @22:10:25.09 Forearm.seek 1 @22:10:26.06 Elbow.hold @22:10:26.13 Elbow.reconfigure :tight @22:10:26.17 Hand.open @22:10:26.91 Forearm.force -20 @22:10:26.95 Forearm.seek 1 + 309 counts @22:10:27.26 Forearm.reconfigure :tight @22:10:27.30 Forearm.seek 1 + 450 counts @22:10:27.56 SE.seek :down @22:10:28.70
Forearm.reconfigure :loose @22:10:28.74 Forearm.seek :retract @22:10:30.18 SE.stop @22:10:30.22
PSV.dial :air @22:10:30.98 PS.seek 2.5ml @22:10:57.39 delay 1 second @22:10:58.41 PSV.dial :mixloop @22:10:58.86 delay 1 second @22:10:59.88 PSV.dial :air @22:11:00.35 delay 1 second @22:11:01.36 PSV.dial :mixloop @22:11:01.83 delay 1 second @22:11:02.84 PSV.dial :air @22:11:03.32 delay 1 second @22:11:04.34 PS.seek 0ml @22:11:30.71 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @22:11:30.89 Elbow.seek FlushPuck::Garage @22:11:32.68 Elbow.reconfigure :loose @22:11:32.78 Forearm.seek :garage @22:11:33.73 Hand.close @22:11:34.40 Forearm.seek :retract @22:11:35.36 Elbow.reconfigure :tight @22:11:35.85 Elbow.seek PC @22:11:37.12 Elbow.reconfigure :loose @22:11:37.22 Forearm.reconfigure :tight @22:11:37.27 Forearm.seek PC @22:11:38.46 PC.seek :closed @22:11:43.07 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.62 @22:11:43.14 Hand.open @22:11:43.84 Forearm.reconfigure :loose @22:11:43.89 Forearm.seek :retract @22:11:44.84 PRV.dial :air @22:11:45.30 PSV.dial :mixloop @22:11:45.83 PS.seek 0.3ml @22:11:49.73 PSV.dial PSR @22:11:50.78 Pulling 0.4ml of PSR.air @22:11:50.79 PSR.select :air @22:11:50.81 PS.seek 0.7ml @22:11:55.77 delay 3 seconds @22:11:58.78 PSR.select :air @22:11:58.80 PS.seek 2.5ml @22:12:18.01 delay 3 seconds @22:12:21.03 PSV.dial :mixloop @22:12:22.08 PTV.dial :mixing @22:12:22.83 PBV.dial :waste2 @22:12:23.79 PS.seek 0ml @22:12:50.23 Garaging FlushPuck in Processing Clamp @22:12:50.30 Elbow.reconfigure :tight @22:12:50.35 Elbow.seek PC @22:12:50.46 Elbow.reconfigure :loose @22:12:50.56 Forearm.seek PC @22:12:51.51 Hand.close @22:12:52.16 PC.seek :open @22:12:56.90 Forearm.seek :retract @22:12:57.86 Elbow.reconfigure :tight @22:12:57.91 Elbow.seek :garage @22:12:59.26 Elbow.reconfigure :loose @22:12:59.36 Forearm.reconfigure :tight @22:12:59.40 Forearm.seek :garage @22:13:00.46 Hand.open @22:13:01.09 Forearm.reconfigure :loose @22:13:01.14 Forearm.seek :retract @22:13:02.10 Loading da puck from tube 2 into Processing Clamp @22:13:02.34 SC.reconfigure :tight @22:13:02.39 SC.seek 2 @22:13:05.94 SC.configure :limp @22:13:06.16 Elbow.reconfigure :tight @22:13:06.21 Elbow.seek 2 @22:13:08.03 Forearm.reconfigure :tight @22:13:08.08 Forearm.seek 2 + 450 counts @22:13:09.17 SE.reconfigure :pick @22:13:09.22 SE.seek :beamBreak @22:13:10.32 SE.configure :default @22:13:10.48 Elbow.seek 2 @22:13:10.67 Forearm.seek 2 + 300 counts @22:13:10.92 Forearm.reconfigure :loose @22:13:10.96 Hand.close @22:13:11.54 SE.seek :down @22:13:11.74
Forearm.seek :retract @22:13:12.30 SE.stop @22:13:12.69
Elbow.seek PC @22:13:15.19 Elbow.reconfigure :loose @22:13:15.74 Forearm.reconfigure :tight @22:13:15.79 Forearm.seek PC @22:13:16.99 PC.seek :closed @22:13:21.62 Processing Clamp onSpring/offSpring for 15/25 samples, on/off torque 1.67 @22:13:21.63 Hand.open @22:13:22.34 Forearm.reconfigure :loose @22:13:22.39 Forearm.seek :retract @22:13:23.33 Heating Puck.from 2, :da in Processing Clamp to 28C for 2:00:00 @22:13:23.35 PH.seek 28C,5:00 @22:13:55.22 PH.hold 2:00:00,28C @22:13:55.71 delay 1:30 @22:15:25.76 PSR.select :air @22:15:25.78 PSV.dial PSR @22:15:26.83 Pulling 0.5ml of PSR.wash PSR.select :wash @22:15:26.95 PS.seek 0.5ml @22:15:32.92 delay 5 seconds @22:15:37.94 PSR.select :air @22:15:38.06 PS.seek 2ml @22:15:54.26 PTV.dial :puck @22:15:55.01 PSV.dial PTV @22:15:55.76 PBV.dial :waste1 @22:15:57.15 PS.seek 1.5ml @22:16:03.10 delay 3 seconds @22:16:06.12 PBV.dial between PSV and waste1 @22:16:06.49 PS.seek 0ml @22:16:22.67 delay 5:00 @22:21:22.68 recoverPSRreagent08 @22:21:22.69 PTV.dial :puck @22:21:22.77 PBV.dial :waste1 @22:21:23.15 delay 30 seconds @22:21:53.17 PBV.dial between PSV and waste1 @22:21:53.53 PSR.select :air @22:21:53.55 PSV.dial PSR @22:21:54.30 PS.reconfigure :fast @22:21:54.35 PS.seek 2.5ml @22:22:18.41 PSV.dial PTV @22:22:19.16 delay 5 seconds @22:22:24.18 PSV.dial PSR @22:22:24.94 delay 5 seconds @22:22:29.96 PSV.dial PTV @22:22:30.71 delay 5 seconds @22:22:35.72 PSV.dial :air @22:22:36.48 PS.seek 0ml @22:22:59.94 PTV.dial :PRVmixing,via: PRV @22:23:01.25 PSV.dial :mixloop @22:23:01.72 PRV.dial :ab1 @22:23:02.48 PS.reconfigure :slow @22:23:02.52 PS.seek 0.15ml @22:23:06.19 delay 2 seconds @22:23:08.21 CBV.dial PRV @22:23:08.24
PRV.dial CBV @22:23:08.80 PS.seek 0.34ml @22:23:13.16 delay 2 seconds @22:23:15.18 CBV.dial between PRV and PSV @22:23:15.49 PRV.dial :air @22:23:15.95 PS.seek 0.9ml @22:23:27.87 PRV.dial :waste2 @22:23:28.62 PS.seek 0.5ml @22:23:37.28 addPMLReagent08 @22:23:37.29 PSV.dial :air @22:23:38.12 PS.reconfigure :default @22:23:38.23 PS.seek 2ml @22:23:54.34 PSV.dial :PML @22:23:54.81 PTV.dial :mixing @22:23:55.56 PBV.dial :waste1 @22:23:55.99 PS.seek 1.85ml @22:23:58.37 PBV.dial between PSV and waste1 @22:23:58.74 PS.seek 0ml @22:24:18.50 delay 2 seconds @22:24:20.52 Beginning samplePassC @22:24:20.53 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @22:24:20.71 Elbow.reconfigure :tight @22:24:20.76 Elbow.seek FlushPuck::Garage @22:24:22.09 Elbow.reconfigure :loose @22:24:22.19 Forearm.seek :garage @22:24:23.21 Hand.close @22:24:23.89 Forearm.seek :retract @22:24:24.86 Elbow.reconfigure :tight @22:24:24.91 Elbow.seek CC @22:24:27.81 Elbow.reconfigure :loose @22:24:27.91 Forearm.reconfigure :tight @22:24:27.96 Forearm.seek CC @22:24:29.20 CC.seek :closed @22:24:33.72 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.46 @22:24:33.78 Hand.open @22:24:34.46 Forearm.reconfigure :loose @22:24:34.95 Forearm.seek :retract @22:24:35.97 PSV.dial PSR @22:24:37.02 Pulling 1.5ml of PSR.flush PSR.select :flush @22:24:37.14 PS.seek 1.5ml @22:24:53.38 delay 5 seconds @22:24:58.40 PSV.dial :waste1 @22:24:58.86 PS.seek 0ml @22:25:15.00 PSV.dial PSR @22:25:15.47 PSR.select :air @22:25:15.58 PS.seek 2.5ml @22:25:41.94 PRV.dial CBV,avoiding: :ab2 @22:25:41.97
CBV.dial PRV @22:25:43.58 PSV.dial :mixloop @22:25:44.61 CTV.dial :air,avoiding: :intake @22:25:44.69 PTV.dial :PRVmixing @22:25:45.46 PS.seek 0ml @22:26:11.87 PSV.dial :air @22:26:12.35 PS.seek 2.5ml @22:26:38.75 PSV.dial :mixloop @22:26:39.22 PS.seek 0ml @22:27:05.59 PSV.dial :air @22:27:06.06 PS.seek 2.5ml @22:27:32.49 PSV.dial :mixloop @22:27:32.97 PS.seek 0ml @22:27:59.37 Garaging FlushPuck in Collection Clamp @22:27:59.44 Elbow.reconfigure :tight @22:27:59.49 Elbow.seek CC @22:27:59.74 Elbow.reconfigure :loose @22:27:59.84 Forearm.seek CC @22:28:00.90 Hand.close @22:28:01.52 CC.seek :open @22:28:06.13 Forearm.seek :retract @22:28:07.17 Elbow.reconfigure :tight @22:28:07.22 Elbow.seek :garage @22:28:10.16 Elbow.reconfigure :loose @22:28:10.26 Forearm.reconfigure :tight @22:28:10.31 Forearm.seek :garage @22:28:11.33 Hand.open @22:28:11.95 Forearm.reconfigure :loose @22:28:12.00 Forearm.seek :retract @22:28:12.97 delayUntil "22:32:20.51" @22:28:13.00 Whole Cell Archive sampling 50ml @22:28:13.01 Loading wcr puck from tube 2 into Collection Clamp @22:28:13.49 Elbow.reconfigure :tight @22:28:13.54 Elbow.seek 2 @22:28:15.46 Forearm.reconfigure :tight @22:28:15.52 Forearm.seek 2 + 450 counts @22:28:16.58 SE.reconfigure :pick @22:28:16.64 SE.seek :beamBreak @22:28:17.78 SE.configure :default @22:28:17.94 Elbow.seek 2 @22:28:18.13 Forearm.seek 2 + 300 counts @22:28:18.29 Forearm.reconfigure :loose @22:28:18.34 Hand.close @22:28:19.03 SE.seek :down @22:28:19.23 Forearm.seek :retract @22:28:19.80 SE.stop @22:28:20.19 Elbow.seek CC @22:28:21.84 Elbow.reconfigure :loose @22:28:21.95 Forearm.reconfigure :tight @22:28:22.00 Forearm.seek CC @22:28:23.75 CC.seek :closed @22:28:28.27 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.23 @22:28:28.29 Hand.open @22:28:28.95 Forearm.reconfigure :loose @22:28:29.00 Forearm.seek :retract @22:28:30.05 collectSample 50ml w/bubble point of 35psi @22:28:30.06 Priming 7ml @22:28:30.07 Intake.select :close @22:28:30.08 Exhaust.select :close @22:28:30.10 SS.hold @22:28:30.30 Calibrating pressure sensors @22:28:30.31 CTV.dial :air @22:28:30.39 SSV.dial :spit,avoiding: :exhaust @22:28:30.42 SS.reconfigure :fast @22:28:30.47 SS.seek 5ml @22:28:33.15 SSV.dial CBV @22:28:33.19 CBV.dial SSV @22:28:34.49 delay 3.8 seconds @22:28:38.43 Sensor limits: Intake max 94.1psia; Outlet max 87.4psia @22:28:38.44 SSV.dial :spit @22:28:39.76 SS.seek 0ml @22:28:42.85 SSV.dial CBV @22:28:44.18 CTV.dial :intake @22:28:44.94 SSV.dial CBV @22:28:44.97 CBV.dial SSV @22:28:45.16 Allowing 40s for 7.0ml of remaining 7.0ml @22:28:45.18 Intake.select :open @22:28:47.35 Intake @14.2psia, vacuum from 8.7 to 11.7psi @22:28:47.37 SS.reconfigure :inhaling 2.20ml/s @22:28:47.42 SS.seek 7ml @22:28:53.38 Stroke completed -- waiting for pressure to equalize ... @22:28:53.39 SS.reconfigure :exhale @22:28:53.44 SS.hold @22:29:08.10 Intake.select :close @22:29:08.26 SSV.dial :exhaust @22:29:09.04 Exhaust.select :open @22:29:09.16 SS.seek 0ml @22:29:13.32 Exhaust.select :close @22:29:13.43 Sampling 50.0ml @22:29:13.44 Intake.select :close @22:29:13.45 Exhaust.select :close @22:29:13.47 SS.reconfigure :default @22:29:13.51 SS.hold @22:29:13.72 CTV.dial :intake @22:29:13.90 SSV.dial CBV @22:29:13.92 CBV.dial SSV @22:29:14.69 Allowing 130s for 25.0ml of remaining 50.0ml @22:29:14.71 Intake.select :open @22:29:16.87 Intake @14.9psia, vacuum from 9.4 to 12.4psi @22:29:16.89 SS.reconfigure :inhaling 2.20ml/s @22:29:16.94 SS.seek 25ml @22:29:28.26 Vacuum too high -- waiting up to 40 seconds @22:29:28.28 SS.reconfigure :exhale @22:29:28.33 SS.hold @22:29:33.57 Intake @13.8psia, vacuum from 8.3 to 11.3psi @22:29:33.59 SS.reconfigure :inhaling 1.76ml/s @22:29:33.64 SS.seek 25ml @22:29:38.67 Stroke completed -- waiting for pressure to equalize ... @22:29:38.69 SS.reconfigure :exhale @22:29:38.73 SS.hold @22:29:44.84 Intake.select :close @22:29:44.99 SSV.dial :exhaust @22:29:45.75 Exhaust.select :open @22:29:45.87 SS.seek 0ml @22:29:54.85 Exhaust.select :close @22:29:54.96 SSV.dial CBV @22:29:54.99 CBV.dial SSV @22:29:55.75 Allowing 130s for 25.0ml of remaining 25.0ml @22:29:55.77 Intake.select :open @22:29:57.94 Intake @15.0psia, vacuum from 9.5 to 12.5psi @22:29:57.96 SS.reconfigure :inhaling 1.76ml/s @22:29:58.01 SS.seek 25ml @22:30:15.16 Stroke completed -- waiting for pressure to equalize ... @22:30:15.17 SS.reconfigure :exhale @22:30:15.22 SS.hold @22:30:23.84 Intake.select :close @22:30:23.99 SSV.dial :exhaust @22:30:24.74 Exhaust.select :open @22:30:24.85 SS.seek 0ml @22:30:33.49 Exhaust.select :close @22:30:34.06 Sampled 50.0ml @22:30:34.08 Drying puck before fixing for 5:00 @22:30:34.09 CBV.dial :waste2b @22:30:34.83 delay 1:00 @22:31:34.86 CSV.dial :air @22:31:35.32 CS.reconfigure :fast @22:31:35.37 CS.seek 10ml @22:31:59.83 Pumping wcr puck from tube 2 up to 35psi... @22:31:59.85 CBV.dial SSV @22:32:01.10 CBV.dial between CSV and waste2b @22:32:02.04 CSV.dial between air and CTV @22:32:02.37 CS.seek 7.363ml @22:32:09.24 CTV.dial CSV,avoiding: :air @22:32:09.27 CSV.dial CTV @22:32:10.08 CS.reconfigure :pressurize @22:32:10.14 CS.seek 0ml @22:32:20.52 recoverPRVreagent08 @22:32:20.53 PTV.dial :mixing @22:32:21.30 PBV.dial :waste1 @22:32:21.68 delay 30 seconds @22:32:29.54 CSV.dial :air @22:32:30.00 CS.reconfigure :fast @22:32:30.06 CS.seek 10ml @22:32:51.70 PBV.dial between PSV and waste1 @22:32:52.08 PSV.dial :air @22:32:52.56 PS.reconfigure :fast @22:32:52.60 PS.seek 2.5ml @22:32:54.35 CSV.dial between air and CTV @22:32:54.69 CS.seek 4.048ml @22:33:08.99 CTV.dial CSV,avoiding: :air @22:33:09.01 CSV.dial CTV @22:33:09.36 CS.reconfigure :pressurize @22:33:09.41 CS.seek 0ml @22:33:16.86 PSV.dial :mixloop @22:33:17.33 delay 2 seconds @22:33:19.35 PSV.dial :air @22:33:19.82 delay 2 seconds @22:33:20.36 CSV.dial :air @22:33:20.81 CS.reconfigure :fast @22:33:20.87 CS.seek 10ml @22:33:21.84 PSV.dial :mixloop @22:33:22.32 delay 2 seconds @22:33:24.33 PSV.dial :air @22:33:24.81 PS.seek 0ml @22:33:45.20 CSV.dial between air and CTV @22:33:45.52 CS.seek 4.14ml @22:33:48.55 washCycleDA #1 of 2 @22:33:48.56 PSR.select :air @22:33:48.58 PSV.dial PSR @22:33:49.91 Pulling 0.5ml of PSR.wash @22:33:49.92 PSR.select :wash @22:33:50.04 PS.reconfigure :default @22:33:50.09 PS.seek 0.5ml @22:33:55.96 delay 5 seconds @22:33:59.64 CTV.dial CSV,avoiding: :air @22:33:59.67 CSV.dial CTV @22:34:00.14 CS.reconfigure :pressurize @22:34:00.20 CS.seek 0ml @22:34:00.97 PSR.select :air @22:34:01.10 PS.seek 2ml @22:34:11.29 CSV.dial :air @22:34:11.74 CS.reconfigure :fast @22:34:11.79 CS.seek 10ml @22:34:17.33 PTV.dial :puck @22:34:18.06 PSV.dial PTV @22:34:18.80 PBV.dial :waste1 @22:34:19.24 PS.seek 1.5ml @22:34:25.17 delay 3 seconds @22:34:28.19 PBV.dial between PSV and waste1 @22:34:28.57 PS.seek 0ml @22:34:36.10 CSV.dial between air and CTV @22:34:36.44 CS.seek 4.157ml @22:34:44.77 delay 2:00 @22:34:50.52 CTV.dial CSV,avoiding: :air @22:34:50.55 CSV.dial CTV @22:34:50.89 CS.reconfigure :pressurize @22:34:50.94 CS.seek 0ml @22:35:02.17 CSV.dial :air @22:35:02.62 CS.reconfigure :default @22:35:02.67 CS.hold @22:35:02.81 ShallowSampler::Error in MAIN -- Puck.from 2, :wcr reached only 20.5 of 35psi after pumping in 40ml of gas @22:35:02.82 Waiting to Resume from Checkpoint "Pumping wcr puck from tube 2 up to 35psi..." @22:35:02.84 BadNews.email "Puck.from 2, :wcr reached only 20.5 of 35psi after pumping in 40ml of gas",Subject: "ShallowSampler::Error in MAIN" @22:35:07.38 upsync @22:36:44.80 recoverPSRreagent08 @22:36:44.81 PTV.dial :puck @22:36:44.83 PBV.dial :waste1 @22:36:45.21 delay 30 seconds @22:37:15.23 PBV.dial between PSV and waste1 @22:37:15.61 PSR.select :air @22:37:15.63 PSV.dial PSR @22:37:16.40 PS.reconfigure :fast @22:37:16.45 PS.seek 2.5ml @22:37:40.76 PSV.dial PTV @22:37:41.53 delay 5 seconds @22:37:46.55 PSV.dial PSR @22:37:47.42 delay 5 seconds @22:37:52.44 PSV.dial PTV @22:37:53.17 delay 5 seconds @22:37:58.19 PSV.dial :air @22:37:58.97 PS.seek 0ml @22:38:22.58 washCycleDA #2 of 2 @22:38:22.59 PSR.select :air @22:38:22.60 PSV.dial PSR @22:38:23.92 Pulling 0.5ml of PSR.wash @22:38:23.93 PSR.select :wash @22:38:24.05 PS.reconfigure :default @22:38:24.10 PS.seek 0.5ml @22:38:30.08 delay 5 seconds @22:38:35.10 PSR.select :air @22:38:35.22 PS.seek 2ml @22:38:51.42 PTV.dial :puck @22:38:51.43 PSV.dial PTV @22:38:52.18 PBV.dial :waste1 @22:38:52.62 PS.seek 1.5ml @22:38:58.57 delay 3 seconds @22:39:01.59 PBV.dial between PSV and waste1 @22:39:01.96 PS.seek 0ml @22:39:18.29 delay 2:00 @22:41:18.32 recoverPSRreagent08 @22:41:18.33 PTV.dial :puck @22:41:18.34 PBV.dial :waste1 @22:41:18.70 delay 30 seconds @22:41:48.72 PBV.dial between PSV and waste1 @22:41:49.09 PSR.select :air @22:41:49.11 PSV.dial PSR @22:41:49.87 PS.reconfigure :fast @22:41:49.92 PS.seek 2.5ml @22:42:14.26 PSV.dial PTV @22:42:15.02 delay 5 seconds @22:42:20.05 PSV.dial PSR @22:42:20.79 delay 5 seconds @22:42:25.81 PSV.dial PTV @22:42:26.68 delay 5 seconds @22:42:31.70 PSV.dial :air @22:42:32.47 PS.seek 0ml @22:42:56.13 getPMLReagent08 @22:42:56.14 PRV.dial :air @22:42:57.06 delay 5 seconds @22:43:02.08 PTV.dial :PRVmixing @22:43:03.41 delay 5 seconds @22:43:08.44 PRV.dial :ab2 @22:43:08.88 PTV.dial :PRVmixing @22:43:08.96 PSV.dial :mixloop @22:43:09.43 PS.reconfigure :default @22:43:09.48 PS.seek 0.3ml @22:43:13.41 delay 2 seconds @22:43:15.43 PRV.dial :air @22:43:15.94 PS.seek 0.55ml @22:43:19.29 addPMLReagent08 @22:43:19.30 PSV.dial :air @22:43:19.76 PS.seek 2ml @22:43:35.35 PSV.dial :PML @22:43:35.82 PTV.dial :mixing @22:43:36.58 PBV.dial :waste1 @22:43:37.01 PS.seek 1.85ml @22:43:39.35 PBV.dial between PSV and waste1 @22:43:39.73 PS.seek 0ml @22:43:59.44 delay 2 seconds @22:44:01.46 delay 8:00 @22:52:01.49 recoverPRVreagent08 @22:52:01.50 PTV.dial :mixing @22:52:01.68 PBV.dial :waste1 @22:52:02.06 delay 30 seconds @22:52:32.09 PBV.dial between PSV and waste1 @22:52:32.45 PSV.dial :air @22:52:32.92 PS.reconfigure :fast @22:52:32.97 PS.seek 2.5ml @22:52:57.34 PSV.dial :mixloop @22:52:57.81 delay 2 seconds @22:52:59.83 PSV.dial :air @22:53:00.29 delay 2 seconds @22:53:02.31 PSV.dial :mixloop @22:53:02.78 delay 2 seconds @22:53:04.80 PSV.dial :air @22:53:05.27 PS.seek 0ml @22:53:28.93 washCycleDA #1 of 3 @22:53:28.94 PSR.select :air @22:53:28.96 PSV.dial PSR @22:53:30.29 Pulling 0.5ml of PSR.wash @22:53:30.30 PSR.select :wash @22:53:30.42 PS.reconfigure :default @22:53:30.47 PS.seek 0.5ml @22:53:36.46 delay 5 seconds @22:53:41.48 PSR.select :air @22:53:41.61 PS.seek 2ml @22:53:57.84 PTV.dial :puck @22:53:58.59 PSV.dial PTV @22:53:59.32 PBV.dial :waste1 @22:53:59.74 PS.seek 1.5ml @22:54:05.69 delay 3 seconds @22:54:08.71 PBV.dial between PSV and waste1 @22:54:09.08 PS.seek 0ml @22:54:25.40 delay 2:00 @22:56:25.43 recoverPSRreagent08 @22:56:25.44 PTV.dial :puck @22:56:25.51 PBV.dial :waste1 @22:56:25.87 delay 30 seconds @22:56:55.89 PBV.dial between PSV and waste1 @22:56:56.32 PSR.select :air @22:56:56.34 PSV.dial PSR @22:56:57.11 PS.reconfigure :fast @22:56:57.16 PS.seek 2.5ml @22:57:21.49 PSV.dial PTV @22:57:22.37 delay 5 seconds @22:57:27.39 PSV.dial PSR @22:57:28.15 delay 5 seconds @22:57:33.17 PSV.dial PTV @22:57:33.91 delay 5 seconds @22:57:38.93 PSV.dial :air @22:57:39.81 PS.seek 0ml @22:58:03.55 washCycleDA #2 of 3 @22:58:03.57 PSR.select :air @22:58:03.58 PSV.dial PSR @22:58:04.91 Pulling 0.5ml of PSR.wash @22:58:04.92 PSR.select :wash @22:58:05.04 PS.reconfigure :default @22:58:05.09 PS.seek 0.5ml @22:58:11.05 delay 5 seconds @22:58:16.07 PSR.select :air @22:58:16.19 PS.seek 2ml @22:58:32.39 PTV.dial :puck @22:58:32.41 PSV.dial PTV @22:58:33.29 PBV.dial :waste1 @22:58:33.72 PS.seek 1.5ml @22:58:39.61 delay 3 seconds @22:58:42.63 PBV.dial between PSV and waste1 @22:58:43.01 PS.seek 0ml @22:58:59.21 delay 2:00 @23:00:59.24 recoverPSRreagent08 @23:00:59.25 PTV.dial :puck @23:00:59.26 PBV.dial :waste1 @23:00:59.63 delay 30 seconds @23:01:29.65 PBV.dial between PSV and waste1 @23:01:30.03 PSR.select :air @23:01:30.05 PSV.dial PSR @23:01:30.91 PS.reconfigure :fast @23:01:30.96 PS.seek 2.5ml @23:01:55.11 PSV.dial PTV @23:01:55.85 delay 5 seconds @23:02:00.87 PSV.dial PSR @23:02:01.61 delay 5 seconds @23:02:06.63 PSV.dial PTV @23:02:07.38 delay 5 seconds @23:02:12.40 PSV.dial :air @23:02:13.29 PS.seek 0ml @23:02:36.86 washCycleDA #3 of 3 @23:02:36.87 PSR.select :air @23:02:36.89 PSV.dial PSR @23:02:38.22 Pulling 0.5ml of PSR.wash @23:02:38.23 PSR.select :wash @23:02:38.35 PS.reconfigure :default @23:02:38.40 PS.seek 0.5ml @23:02:44.61 delay 5 seconds @23:02:49.63 PSR.select :air @23:02:49.75 PS.seek 2ml @23:03:05.98 PTV.dial :puck @23:03:06.00 PSV.dial PTV @23:03:06.88 PBV.dial :waste1 @23:03:07.31 PS.seek 1.5ml @23:03:13.36 delay 3 seconds @23:03:16.38 PBV.dial between PSV and waste1 @23:03:16.91 PS.seek 0ml @23:03:33.08 delay 2:00 @23:05:33.10 recoverPSRreagent08 @23:05:33.11 PTV.dial :puck @23:05:33.13 PBV.dial :waste1 @23:05:33.48 delay 30 seconds @23:06:03.51 PBV.dial between PSV and waste1 @23:06:03.87 PSR.select :air @23:06:03.89 PSV.dial PSR @23:06:04.75 PS.reconfigure :fast @23:06:04.80 PS.seek 2.5ml @23:06:29.07 PSV.dial PTV @23:06:29.81 delay 5 seconds @23:06:34.83 PSV.dial PSR @23:06:35.58 delay 5 seconds @23:06:40.60 PSV.dial PTV @23:06:41.34 delay 5 seconds @23:06:46.36 PSV.dial :air @23:06:47.14 PS.seek 0ml @23:07:10.67 Gate.power :camera,:ON @23:07:10.68 Mixing DA Substrates @23:07:10.69 PSR.select :air @23:07:10.71 PSV.dial PSR @23:07:12.07 Pulling 0.25ml of PSR.sub1 @23:07:12.10 PSR.select :sub1 @23:07:12.32 PS.reconfigure :default @23:07:12.44 PS.seek 0.25ml @23:07:15.89 delay 5 seconds @23:07:20.92 Pulling 0.25ml of PSR.sub2 @23:07:20.93 PSR.select :sub2 @23:07:21.05 PS.seek 0.5ml @23:07:24.42 delay 5 seconds @23:07:29.44 PSR.select :air @23:07:29.55 PS.seek 2ml @23:07:45.70 PTV.dial :puck @23:07:45.71 PSV.dial PTV @23:07:46.46 PBV.dial :waste1 @23:07:46.90 PS.seek 1.5ml @23:07:52.95 delay 3 seconds @23:07:55.97 PBV.dial between PSV and waste1 @23:07:56.34 PS.seek 0ml @23:08:12.45 delay 10 seconds @23:08:22.47 recoverPSRreagent08 @23:08:22.48 PTV.dial :puck @23:08:22.49 PBV.dial :waste1 @23:08:22.87 delay 30 seconds @23:08:52.90 PBV.dial between PSV and waste1 @23:08:53.27 PSR.select :air @23:08:53.29 PSV.dial PSR @23:08:54.06 PS.reconfigure :fast @23:08:54.11 PS.seek 2.5ml @23:09:18.73 PSV.dial PTV @23:09:19.49 delay 5 seconds @23:09:24.51 PSV.dial PSR @23:09:25.39 delay 5 seconds @23:09:30.41 PSV.dial PTV @23:09:31.17 delay 5 seconds @23:09:36.19 PSV.dial :air @23:09:36.97 PS.seek 0ml @23:10:01.14 Additional air clear @23:10:01.15 PSR.select :air @23:10:01.16 PSV.dial PSR @23:10:02.51 Pulling 2ml of PSR.air @23:10:02.52 PSR.select :air @23:10:02.54 PS.reconfigure :default @23:10:02.59 PS.seek 2ml @23:10:23.82 delay 5 seconds @23:10:28.84 PTV.dial :puck @23:10:28.86 PSV.dial PTV @23:10:29.60 PBV.dial :waste1 @23:10:30.51 PS.seek 1.5ml @23:10:36.41 delay 3 seconds @23:10:39.43 PBV.dial between PSV and waste1 @23:10:39.81 delay 1 second @23:10:40.82 PSV.dial :waste1 @23:10:41.90 PS.seek 0ml @23:10:58.06 Processing Puck Heater at 28C before imaging @23:10:58.07 PH.coast @23:10:58.18 Imaging da puck in Processing Clamp while storing it to tube 1 @23:10:58.36 SC.reconfigure :tight @23:10:58.41 SC.seek 1 @23:11:01.97 SC.configure :limp @23:11:02.19 Elbow.reconfigure :tight @23:11:02.24 Elbow.seek 1 @23:11:04.02 Forearm.reconfigure :tight @23:11:04.07 Forearm.seek 1 + 450 counts @23:11:05.17 SE.reconfigure :pick @23:11:05.22 SE.seek :beamBreak @23:11:08.30 SE.configure :default @23:11:08.46 Elbow.reconfigure :limp @23:11:08.51 Forearm.reconfigure :loose @23:11:08.56 Forearm.seek 1 @23:11:08.77 Elbow.hold @23:11:08.84 Elbow.reconfigure :tight @23:11:08.95 SE.seek 1.17 Pucks below Top Plate @23:11:09.39 Forearm.force -20 @23:11:09.44 Forearm.seek 1 + 308 counts @23:11:09.74 Forearm.reconfigure :tight @23:11:09.79 Forearm.seek 1 + 450 counts @23:11:10.00 SE.seek 2.17 Pucks below Top Plate @23:11:10.67 Forearm.reconfigure :loose @23:11:10.72 Forearm.seek :retract @23:11:11.64 Elbow.seek PC @23:11:14.34 Elbow.reconfigure :loose @23:11:14.87 Forearm.seek PC @23:11:15.90 Hand.close @23:11:16.48 PC.seek :open @23:11:21.24 Forearm.seek :retract @23:11:22.22 Elbow.reconfigure :tight @23:11:22.27 Elbow.seek 1 @23:11:24.91 Forearm.reconfigure :tight @23:11:24.96 Forearm.seek 1 + 450 counts @23:11:25.91 Hand.open @23:11:26.54 SE.seek 1.82 Pucks below Top Plate @23:11:26.82 SE.reconfigure :seal @23:11:26.87 SE.seek 0.85 Pucks below Top Plate @23:11:27.43 Lights, camera, ACTION! @23:11:27.46 PuckCamera: snap -bin=2,2 -auto /var/log/esp/da23sep1823h50ml.tif @23:11:46.92 Waiting >20 seconds for da to relinquish FlushPuck @23:11:51.66 SX HX9/16 (USB:02): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 20.719 seconds /var/log/esp/da23sep1823h50ml.tif: TIFF Upload Complete @23:11:51.69 PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da23sep1823h50ml15s.tif @23:12:07.30 SX HX9/16 (USB:02): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15 seconds /var/log/esp/midres/da23sep1823h50ml15s.tif: TIFF Upload Complete @23:12:07.33 PuckCamera: snap -bin=2,2 20.0 /var/log/esp/midres/da23sep1823h50ml20s.tif @23:12:27.98 SX HX9/16 (USB:02): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 20 seconds /var/log/esp/midres/da23sep1823h50ml20s.tif: TIFF Upload Complete @23:12:27.99 Gate.power :camera,:OFF @23:12:28.01 DAfans.email "20.719 sec auto-exposure",Subject: "da23sep1823h50ml images!" @23:12:28.54 Elbow.seek 1 @23:12:29.06 Forearm.seek 1 + 450 counts @23:12:29.41 SE.reconfigure :gently @23:12:29.48 SE.seek 1.57 Pucks below Top Plate @23:12:30.14 Elbow.reconfigure :limp @23:12:30.19 Forearm.reconfigure :loose @23:12:30.24 Forearm.seek 1 @23:12:30.51 Elbow.hold @23:12:30.58 Elbow.reconfigure :tight @23:12:30.63 SE.reconfigure :default @23:12:30.68 SE.seek 1.75 Pucks below Top Plate @23:12:31.03 Forearm.force -20 @23:12:31.08 Forearm.seek 1 + 313 counts @23:12:32.31 Forearm.reconfigure :tight @23:12:32.41 Forearm.seek 1 + 450 counts @23:12:32.85 SE.seek :down @23:12:34.12 Forearm.reconfigure :loose @23:12:34.25 Forearm.seek :retract @23:12:35.49 SE.stop @23:12:35.69 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @23:12:36.07 Elbow.seek FlushPuck::Garage @23:12:38.11 Elbow.reconfigure :loose @23:12:38.33 Forearm.seek :garage @23:12:39.37 Hand.close @23:12:40.32 Forearm.seek :retract @23:12:41.40 Elbow.reconfigure :tight @23:12:41.53 Elbow.seek PC @23:12:42.91 Elbow.reconfigure :loose @23:12:43.11 Forearm.reconfigure :tight @23:12:43.21 Forearm.seek PC @23:12:44.85 PC.seek :closed @23:12:49.62 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.68 @23:12:49.80 Hand.open @23:12:50.64 Forearm.reconfigure :loose @23:12:50.73 Forearm.seek :retract @23:12:51.76 PSR.select :air @23:12:51.84 PSV.dial PSR @23:12:52.38 Pulling 0.5ml of PSR.flush @23:12:52.40 PSR.select :flush @23:12:52.57 PS.seek 0.5ml @23:12:59.64 delay 5 seconds @23:13:01.90 upsync @23:13:04.68 PSR.select :air @23:13:04.81 PS.seek 2.5ml @23:13:26.00 delay 1 second @23:13:27.02 PRV.dial :waste2,avoiding: :ab2 @23:13:28.89 PTV.dial :PRVmixing @23:13:30.21 PSV.dial :mixloop @23:13:31.25 PS.seek :empty @23:13:57.66 PSR.select :air @23:13:57.68 PSV.dial PSR @23:13:58.72 Pulling 0.5ml of PSR.flush @23:13:58.73 PSR.select :flush @23:13:58.85 PS.seek 0.5ml @23:14:04.75 delay 5 seconds @23:14:09.77 PSR.select :air @23:14:09.89 PS.seek 2.5ml @23:14:31.19 delay 1 second @23:14:32.21 PBV.dial :waste1 @23:14:32.58 PTV.dial :mixing @23:14:33.33 PSV.dial :mixloop @23:14:34.40 PS.seek :empty @23:15:00.78 PSV.dial PSR @23:15:01.83 PSR.select :flush @23:15:01.95 PS.seek 1ml @23:15:13.06 PSR.select :air @23:15:13.18 PS.seek 2.5ml @23:15:29.34 PSV.dial PTV @23:15:30.09 PTV.dial :puck @23:15:30.83 PBV.dial :waste1 @23:15:30.91 PS.seek 0ml @23:15:57.25 PSV.dial PSR @23:15:58.00 PSR.select :flush @23:15:58.12 PS.seek 0.5ml @23:16:04.06 PSR.select :air @23:16:04.18 PS.seek 2.5ml @23:16:25.50 PSV.dial PTV @23:16:26.25 PBV.dial PSV @23:16:26.82 PS.seek 0ml @23:16:53.18 PSV.dial PBV @23:16:53.66 PS.seek 2.5ml @23:17:19.97 PSV.dial :waste1 @23:17:20.71 PS.seek 0ml @23:17:47.03 PSV.dial PSR @23:17:47.50 PSR.select :air @23:17:47.52 PS.seek 2.5ml @23:18:13.87 PSV.dial PTV @23:18:14.61 PBV.dial PSV @23:18:14.80 PS.seek 0ml @23:18:41.81 PSV.dial PBV @23:18:42.28 PS.seek 2.5ml @23:19:08.65 PSV.dial :waste1 @23:19:09.39 PS.seek 0ml @23:19:35.74 PSV.dial PSR @23:19:36.21 PSR.select :flush @23:19:36.34 PS.seek 1ml @23:19:47.43 PSR.select :air @23:19:47.55 PS.seek 2.5ml @23:20:03.71 PSV.dial PTV @23:20:04.45 PTV.dial :puck @23:20:04.53 PBV.dial :waste1 @23:20:05.08 PS.seek 0ml @23:20:31.59 PSV.dial PSR @23:20:32.34 PSR.select :flush @23:20:32.46 PS.seek 0.5ml @23:20:38.56 PSR.select :air @23:20:38.68 PS.seek 2.5ml @23:20:59.99 PSV.dial PTV @23:21:00.87 PBV.dial PSV @23:21:01.44 PS.seek 0ml @23:21:27.80 PSV.dial PBV @23:21:28.26 PS.seek 2.5ml @23:21:54.83 PSV.dial :waste1 @23:21:55.58 PS.seek 0ml @23:22:21.87 PSV.dial PSR @23:22:22.34 PSR.select :air @23:22:22.36 PS.seek 2.5ml @23:22:48.68 PSV.dial PTV @23:22:49.41 PBV.dial PSV @23:22:49.61 PS.seek 0ml @23:23:15.96 PSV.dial PBV @23:23:16.44 PS.seek 2.5ml @23:23:42.83 PSV.dial :waste1 @23:23:43.58 PS.seek 0ml @23:24:09.96 Garaging FlushPuck in Processing Clamp @23:24:10.03 Elbow.reconfigure :tight @23:24:10.08 Elbow.seek PC @23:24:10.21 Elbow.reconfigure :loose @23:24:10.31 Forearm.seek PC @23:24:11.29 Hand.close @23:24:12.35 PC.seek :open @23:24:17.14 Forearm.seek :retract @23:24:18.11 Elbow.reconfigure :tight @23:24:18.16 Elbow.seek :garage @23:24:19.49 Elbow.reconfigure :loose @23:24:19.59 Forearm.reconfigure :tight @23:24:19.64 Forearm.seek :garage @23:24:20.68 Hand.open @23:24:21.40 Forearm.reconfigure :loose @23:24:21.45 Forearm.seek :retract @23:58:10.50 Session Begun @23:58:19.07 -> Flush FlushPuck is in FlushPuck::Garage @23:58:21.48 -> CC Collection Clamp contains wcr puck from tube 2 @23:58:37.37 -> PC Processing Clamp open @00:00:48.16UTC19-Sep-23 -> CC.unloadPuck 1, :WCR @00:00:48.25 Puck::Warning in dave -- Unloading Puck.from 2, :wcr to Storage Carousel @00:00:48.27 Storing WCR puck in Collection Clamp to tube 1 @00:00:48.48 SC.reconfigure :tight @00:00:48.55 SC.seek 1 @00:00:48.70 SC.configure :limp @00:00:48.93 Elbow.reconfigure :tight @00:00:49.00 Elbow.seek 1 @00:00:50.87 Forearm.reconfigure :tight @00:00:50.95 Forearm.seek 1 + 450 counts @00:00:51.94 SE.reconfigure :pick @00:00:52.02 SE.seek :beamBreak @00:00:54.98 SE.configure :default @00:00:55.16 Elbow.reconfigure :limp @00:00:55.23 Forearm.reconfigure :loose @00:00:55.30 Forearm.seek 1 @00:00:55.58 Elbow.hold @00:00:55.66 Elbow.reconfigure :tight @00:00:55.78 SE.seek 2.17 Pucks below Top Plate @00:00:56.26 Forearm.force -20 @00:00:56.32 Forearm.seek 1 + 309 counts @00:00:56.64 Forearm.reconfigure :tight @00:00:56.71 Forearm.seek 1 + 450 counts @00:00:56.93 SE.seek 3.17 Pucks below Top Plate @00:00:57.45 Forearm.reconfigure :loose @00:00:57.91 Forearm.seek :retract @00:00:58.94 Elbow.seek CC @00:01:00.57 Elbow.reconfigure :loose @00:01:00.69 Forearm.seek CC @00:01:01.72 Hand.close @00:01:02.32 CC.seek :open @00:01:07.09 Forearm.seek :retract @00:01:08.07 Elbow.reconfigure :tight @00:01:08.14 Elbow.seek 1 @00:01:09.91 Elbow.reconfigure :limp @00:01:09.98 Forearm.seek 1 @00:01:11.00 Elbow.hold @00:01:11.09 Elbow.reconfigure :tight @00:01:11.16 Hand.open @00:01:11.94 Forearm.force -20 @00:01:12.00 Forearm.seek 1 + 314 counts @00:01:12.34 Forearm.reconfigure :tight @00:01:12.41 Forearm.seek 1 + 450 counts @00:01:12.69 SE.seek :down @00:01:13.84 Forearm.reconfigure :loose @00:01:13.91 Forearm.seek :retract @00:01:15.21 SE.stop @00:01:40.97 -> Puck.count @00:01:41.06 SE.seek :down @00:01:41.14 SE.stop @00:01:41.25 SC.reconfigure :tight @00:01:41.32 SC.seek 1 @00:01:41.45 SC.configure :limp @00:01:41.63 Elbow.seek 1 @00:01:41.83 Forearm.reconfigure :tight @00:01:41.90 Forearm.seek 1 + 450 counts @00:01:42.93 SE.reconfigure :place @00:01:43.01 SE.seek :beamBreak @00:01:46.10 SE.configure :default @00:01:46.23 SE.seek :down @00:01:47.74 Forearm.reconfigure :loose @00:01:47.88 Forearm.seek :retract @00:01:48.70 SE.stop @00:01:48.87 SC.reconfigure :tight @00:01:48.94 SC.seek 2 @00:01:52.52 SC.configure :limp @00:01:52.70 Elbow.seek 2 @00:01:52.91 Forearm.reconfigure :tight @00:01:52.98 Forearm.seek 2 + 450 counts @00:01:54.04 SE.reconfigure :place @00:01:54.12 SE.seek :beamBreak @00:01:55.56 SE.configure :default @00:01:55.69 SE.seek :down @00:01:56.46 SE.stop @00:01:56.82 Forearm.reconfigure :loose @00:01:56.90 Forearm.seek :retract @00:01:57.87 SC.reconfigure :tight @00:01:57.94 SC.seek 3 @00:02:01.46 SC.configure :limp @00:02:01.64 Elbow.seek 3 @00:02:02.18 Forearm.reconfigure :tight @00:02:02.25 Forearm.seek 3 + 450 counts @00:02:03.35 SE.reconfigure :place @00:02:03.43 SE.seek :beamBreak @00:02:04.57 SE.configure :default @00:02:04.70 SE.seek :down @00:02:05.13 SE.stop @00:02:05.83 Forearm.reconfigure :loose @00:02:05.90 Forearm.seek :retract @00:02:06.90 SC.reconfigure :tight @00:02:06.97 SC.seek 4 @00:02:10.57 SC.configure :limp @00:02:10.75 Elbow.seek 4 @00:02:11.27 Forearm.reconfigure :tight @00:02:11.34 Forearm.seek 4 + 450 counts @00:02:12.41 SE.reconfigure :place @00:02:12.48 SE.seek :beamBreak @00:02:14.09 SE.configure :default @00:02:14.22 SE.seek :down @00:02:14.65 SE.stop @00:02:15.36 Forearm.reconfigure :loose @00:02:15.43 Forearm.seek :retract @00:02:16.37 SC.reconfigure :tight @00:02:16.44 SC.seek 5 @00:02:20.01 SC.configure :limp @00:02:20.19 Elbow.seek 5 @00:02:20.38 Forearm.reconfigure :tight @00:02:20.46 Forearm.seek 5 + 450 counts @00:02:21.64 SE.reconfigure :place @00:02:21.72 SE.seek :beamBreak @00:02:22.87 SE.configure :default @00:02:23.00 SE.seek :down @00:02:23.44 SE.stop @00:02:24.13 Forearm.reconfigure :loose @00:02:24.20 Forearm.seek :retract @00:02:25.18 SC.reconfigure :tight @00:02:25.25 SC.seek 6 @00:02:28.81 SC.configure :limp @00:02:29.09 Elbow.seek 6 @00:02:29.30 Forearm.reconfigure :tight @00:02:29.37 Forearm.seek 6 + 450 counts @00:02:30.42 SE.reconfigure :place @00:02:30.50 SE.seek :beamBreak @00:02:31.71 SE.configure :default @00:02:31.84 SE.seek :down @00:02:32.26 SE.stop @00:02:32.97 Forearm.reconfigure :loose @00:02:33.04 Forearm.seek :retract @00:02:34.03 SC.reconfigure :tight @00:02:34.10 SC.seek 7 @00:02:37.63 SC.configure :limp @00:02:37.81 Elbow.seek 7 @00:02:38.31 Forearm.reconfigure :tight @00:02:38.83 Forearm.seek 7 + 450 counts @00:02:39.88 SE.reconfigure :place @00:02:39.96 SE.seek :beamBreak @00:02:41.31 SE.configure :default @00:02:41.44 SE.seek :down @00:02:42.07 SE.stop @00:02:42.58 Forearm.reconfigure :loose @00:02:42.65 Forearm.seek :retract {1 => 4, 2 => 29, 3 => 33, 4 => 33, 5 => 33, 6 => 33, 7 => 31} @00:13:27.81 -> exit @00:13:27.83 Session Ended + ESPwhich DA_WCR_91823b.rb + fn=DA_WCR_91823b.rb + IFS=: + set /home/esp/esp2/mission . + fullPath=/home/esp/esp2/mission/DA_WCR_91823b.rb + [ -f /home/esp/esp2/mission/DA_WCR_91823b.rb ] + realpath /home/esp/esp2/mission/DA_WCR_91823b.rb + return 0 + scriptPath=/home/esp/esp2local/mission/DA_WCR_91823b.rb + exec ruby /home/esp/esp2/lib/esp.rb /home/esp/esp2local/mission/DA_WCR_91823b.rb @17:37:09.92UTC19-Sep-23
ESP2 v3.42 I2C Gateway v3.20 for core bus @17:37:12.57 Configuration for short puck jake 1/11/21 by brent@mbari.org /home/esp/esp2/type/shallow/jake/configure.rb @17:37:16.15 Polling Can every 10:00 @17:37:16.19 Cleanly exited at 17:13:38UTC19-Sep-23 @17:37:16.23 Loading /home/esp/esp2local/mission/DA_WCR_91823b.rb mission @17:37:16.29 GoodNews.email "Starting DA_WCR_91823b w/pucks in tube 2\n{1 => 4,\n 2 => 29,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@17:37:16, 25% flow, 21.9C, 12% humidity, 14.8psia, 12.116V, 0.290A, 0.153Ah, 3.52W",Subject: "DA_WCR_91823b begun" @17:37:16.33
Preparing to sleep until + 00:00:05 @17:37:16.36 Asleep til + 00:00:05 @17:37:16.38 Continuing at 17:37 @17:37:16.66 Gate.power :core,:OFF @17:37:16.68 Gate.power :raw,:OFF @17:37:16.69 Shutdown @17:37:19.23 GoodNews.email "Then, in tube 2: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_91823b.rb:14\n{1 => 4,\n 2 => 29,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@17:37:16, 25% flow, 21.9C, 12% humidity, 14.8psia, 12.116V, 0.296A, 0.153Ah, 3.59W",Subject: "Asleep until 17:37" @17:37:20.73 upsync @17:37:21.28
Powering core bus @17:37:23.27 upsync @17:37:27.05
Gate.power :raw,:ON @17:37:28.10 CS.configure :default @17:37:28.15 PS.configure :default @17:37:28.20 SS.configure :default @17:37:28.25 CC.configure :default @17:37:28.29 PC.configure :default @17:37:28.36 Forearm.configure :loose @17:37:28.41 Elbow.configure :loose @17:37:28.47 SC.configure :loose @17:37:28.52 SE.configure :default @17:37:28.57 CH.configure :default @17:37:28.60 PH.configure :default @17:37:28.64 All dwarf controllers running firmware version 3.85 @17:37:28.75 Valve.reset @17:37:29.04 Intake.select :close @17:37:29.05 Exhaust.select :close @17:37:29.06 CTV.dial :air @17:37:29.14 PSV.dial :air @17:37:29.27 CSV.dial :waste1 @17:37:29.30
Forearm.home.seek :retract @17:37:29.40 PS.home.seek 0ml @17:37:29.79 SSV.dial :spit,avoiding: :exhaust @17:37:29.97 CS.reconfigure :fast @17:37:30.01 SS.home.seek 0ml @17:37:30.08 CS.home.seek 0ml @17:37:30.17 Hand.close @17:37:30.20
Elbow.reconfigure :loose @17:37:30.28 Elbow.jog 1500 @17:37:30.76 Elbow.reconfigure :loose @17:37:30.81 Elbow.home.seek :clear @17:37:34.08 SE.home.seek :down @17:37:34.74 SC.reconfigure :tight @17:37:34.79 SC.home.seek :initial @17:37:37.87 PS.stop @17:38:03.22
SC.configure :limp @17:38:03.28 Forearm.seek :retract @17:38:03.34 Elbow.seek :hammer @17:38:04.37 Forearm.reconfigure :tight @17:38:04.42 Forearm.seek retracted @17:38:04.69 CC.home.seek :open @17:38:04.73 PC.home.seek :open @17:38:08.58
Phase [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] @17:38:08.60 Notice.email "At 17:38:08 began in tube 2: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_91823b.rb:14\n{1 => 4,\n 2 => 29,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@17:38:08, 25% flow, 21.9C, 12% humidity, 14.8psia, 12.121V, 0.333A, 0.159Ah, 4.03W",Subject: "Started DA_WCR_91823b phase initialPurge; da [1000, 1000] #da and wsr are run simultanelously" @17:38:08.62
Starting initialPurge @17:38:08.65 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @17:38:08.71 Hand.open @17:38:09.58 Elbow.reconfigure :tight @17:38:09.65 Elbow.seek FlushPuck::Garage @17:38:11.62 Elbow.reconfigure :loose @17:38:11.79 Forearm.reconfigure :loose @17:38:11.85 Forearm.seek :garage @17:38:12.81 Hand.close @17:38:12.86 upsync @17:38:13.72
Forearm.seek :retract @17:38:14.70 Elbow.reconfigure :tight @17:38:14.75 Elbow.seek CC @17:38:17.71 Elbow.reconfigure :loose @17:38:17.81 Forearm.reconfigure :tight @17:38:17.86 Forearm.seek CC @17:38:19.07 CC.seek :closed @17:38:23.76 Collection Clamp onSpring/offSpring for 15/26 samples, on/off torque 3.35 @17:38:23.83 Hand.open @17:38:24.65 Forearm.reconfigure :loose @17:38:24.69 Forearm.seek :retract @17:38:25.70 Flushing the Collection Valve::Manifold @17:38:25.82 CSV.dial CSR @17:38:27.27 Pulling 5ml of CSR.flush @17:38:27.28 CSR.select :flush @17:38:27.40 CS.reconfigure :default @17:38:27.85 CS.seek 5ml @17:38:42.63 delay 5 seconds @17:38:47.65 CSR.select :air @17:38:47.82 CS.reconfigure :fast @17:38:47.87 CS.seek 7.5ml @17:38:55.51 CSV.dial :waste @17:38:56.97 CS.reconfigure :default @17:38:57.01 CS.seek 0ml @17:39:17.32 Purging external Intake @17:39:17.44 CSV.dial :flush @17:39:17.92 CS.reconfigure :fast @17:39:17.97 CS.seek 10ml @17:39:46.16 delay 5 seconds @17:39:51.18 CSV.dial CBV @17:39:51.23
CBV.dial CSV @17:39:52.04 CTV.dial :intake @17:39:52.91 Intake.select :open @17:39:53.02 CS.seek 0ml @17:40:19.91 Intake.select :close @17:40:20.01 CTV.dial :air @17:40:20.88 Flushing upper manifold @17:40:21.00 CSV.dial :flush @17:40:21.82 CS.seek 5ml @17:40:36.25 delay 5 seconds @17:40:41.27 CSV.dial :air @17:40:41.83 CS.seek 6.999ml @17:40:47.94 CSV.dial CBV @17:40:47.97
CBV.dial CSV @17:40:49.14 CTV.dial :air @17:40:49.16 CS.seek 0ml @17:41:08.16 delay 7 seconds @17:41:15.19 CS.seek 3ml @17:41:24.35 CSV.dial :waste @17:41:24.85 CS.seek 0ml @17:41:33.58 CSV.dial :flush @17:41:34.06 CS.seek 3ml @17:41:43.19 delay 5 seconds @17:41:48.21 CSV.dial :air @17:41:48.77 CS.seek 5ml @17:41:55.13 CSV.dial :air @17:41:55.31 CS.seek 0ml @17:42:09.13 delay 7 seconds @17:42:16.16 CS.seek 3ml @17:42:25.25 CSV.dial :waste @17:42:26.07 CS.seek 0ml @17:42:34.75 Garaging FlushPuck in Collection Clamp @17:42:34.82 Elbow.reconfigure :tight @17:42:34.87 Elbow.seek CC @17:42:35.14 Elbow.reconfigure :loose @17:42:35.24 Forearm.seek CC @17:42:36.26 Hand.close @17:42:36.96 CC.seek :open @17:42:42.05 Forearm.seek :retract @17:42:43.04 Elbow.reconfigure :tight @17:42:43.09 Elbow.seek :garage @17:42:46.08 Elbow.reconfigure :loose @17:42:46.18 Forearm.reconfigure :tight @17:42:46.22 Forearm.seek :garage @17:42:47.25 Hand.open @17:42:48.02 Forearm.reconfigure :loose @17:42:48.06 Forearm.seek :retract @17:42:49.06 da sampling at most 1000ml, wcr at most 1000ml @17:42:49.09 DAfans.email "Started da in tube 2 at 17:42:49UTC19-Sep-23\n{1 => 4,\n 2 => 29,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@17:38:08, 25% flow, 21.9C, 12% humidity, 14.8psia, 12.121V, 0.333A, 0.159Ah, 4.03W",Subject: "da sampling at most 1000ml, wcr at most 1000ml" @17:42:49.13
Loading sh1 puck from tube 2 into Collection Clamp @17:42:49.37 SC.reconfigure :tight @17:42:49.43 SC.seek 2 @17:42:54.03 upsync @17:42:56.38
SC.configure :limp @17:42:56.61 Elbow.reconfigure :tight @17:42:56.66 Elbow.seek 2 @17:42:58.60 Forearm.reconfigure :tight @17:42:58.65 Forearm.seek 2 + 450 counts @17:42:59.73 SE.reconfigure :pick @17:42:59.79 SE.seek :beamBreak @17:43:01.13 SE.configure :default @17:43:01.29 Elbow.seek 2 @17:43:01.48 Forearm.seek 2 + 300 counts @17:43:01.74 Forearm.reconfigure :loose @17:43:01.79 Hand.close @17:43:02.60 SE.seek :down @17:43:02.81
Forearm.seek :retract @17:43:03.47 SE.stop @17:43:04.21
Elbow.seek CC @17:43:05.86 Elbow.reconfigure :loose @17:43:05.96 Forearm.reconfigure :tight @17:43:06.01 Forearm.seek CC @17:43:07.32 CC.seek :closed @17:43:11.94 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 3.07 @17:43:11.95 Hand.open @17:43:12.77 Forearm.reconfigure :loose @17:43:12.81 Forearm.seek :retract @17:43:13.84 collectSample 1000ml w/bubble point of 24psi @17:43:13.85 Priming 7ml @17:43:13.86 Intake.select :close @17:43:13.87 Exhaust.select :close @17:43:13.88 SS.hold @17:43:14.09 Calibrating pressure and temperature sensors @17:43:14.11 CTV.dial :air @17:43:14.13 SSV.dial :spit,avoiding: :exhaust @17:43:14.15 SS.reconfigure :fast @17:43:14.19 SS.seek 5ml @17:43:17.33 SSV.dial CBV @17:43:17.37
CBV.dial SSV @17:43:18.86 delay 3.8 seconds @17:43:22.79 Sensor limits: Intake max 112.3psia; Outlet max 106.1psia @17:43:22.80 SSV.dial :spit @17:43:24.27 SS.seek 0ml @17:43:26.92 SSV.dial CBV @17:43:28.39 CTV.dial :intake @17:43:29.27 SSV.dial CBV @17:43:29.30
CBV.dial SSV @17:43:29.44 Allowing 40s for 7.0ml of remaining 7.0ml @17:43:29.45 Intake.select :open @17:43:31.61 Intake @14.3psia, vacuum from 8.8 to 11.8psi @17:43:31.63 SS.reconfigure :inhaling 2.20ml/s @17:43:31.68 SS.seek 7ml @17:43:38.70 Stroke completed -- waiting for pressure to equalize ... @17:43:38.71 SS.reconfigure :exhale @17:43:38.76 SS.hold @17:43:43.93 Intake.select :close @17:43:44.08 SSV.dial :exhaust @17:43:44.90 Exhaust.select :open @17:43:45.02 SS.seek 0ml @17:43:49.25 Exhaust.select :close @17:43:49.36 Sampling 1000.0ml @17:43:49.37 Intake.select :close @17:43:49.38 Exhaust.select :close @17:43:49.39 SS.reconfigure :default @17:43:49.43 SS.hold @17:43:49.63 CTV.dial :intake @17:43:49.71 SSV.dial CBV @17:43:49.74
CBV.dial SSV @17:43:50.56 Allowing 130s for 25.0ml of remaining 1000.0ml @17:43:50.57 Intake.select :open @17:43:52.73 Intake @14.9psia, vacuum from 9.4 to 12.4psi @17:43:52.75 SS.reconfigure :inhaling 2.20ml/s @17:43:52.80 SS.seek 25ml @17:44:05.25 Vacuum too high -- waiting up to 40 seconds @17:44:05.27 SS.reconfigure :exhale @17:44:05.32 SS.hold @17:44:13.16 Intake @16.7psia, vacuum from 10.0 to 13.0psi @17:44:13.18 SS.reconfigure :inhaling 1.76ml/s @17:44:13.22 SS.seek 25ml @17:44:17.84 Stroke completed -- waiting for pressure to equalize ... @17:44:17.85 SS.reconfigure :exhale @17:44:17.89 SS.hold @17:44:30.84 Intake.select :close @17:44:31.00 SSV.dial :exhaust @17:44:31.83 Exhaust.select :open @17:44:31.94 SS.seek 0ml @17:44:40.75 Exhaust.select :close @17:44:40.86 SSV.dial CBV @17:44:40.88
CBV.dial SSV @17:44:41.72 Allowing 130s for 25.0ml of remaining 975.0ml @17:44:41.73 Intake.select :open @17:44:43.90 Intake @15.4psia, vacuum from 9.9 to 12.9psi @17:44:43.91 SS.reconfigure :inhaling 1.76ml/s @17:44:43.96 SS.seek 25ml @17:44:58.53 Vacuum too high -- waiting up to 40 seconds @17:44:58.55 SS.reconfigure :exhale @17:44:58.60 SS.hold @17:45:05.89 Intake @16.2psia, vacuum from 10.0 to 13.0psi @17:45:05.90 SS.reconfigure :inhaling 1.41ml/s @17:45:05.95 SS.seek 25ml @17:45:10.51 Stroke completed -- waiting for pressure to equalize ... @17:45:10.52 SS.reconfigure :exhale @17:45:10.57 SS.hold @17:45:23.20 Intake.select :close @17:45:23.35 SSV.dial :exhaust @17:45:24.18 Exhaust.select :open @17:45:24.30 SS.seek 0ml @17:45:33.20 Exhaust.select :close @17:45:33.31 SSV.dial CBV @17:45:33.33
CBV.dial SSV @17:45:34.17 Allowing 130s for 25.0ml of remaining 950.0ml @17:45:34.18 Intake.select :open @17:45:36.34 Intake @15.6psia, vacuum from 10.0 to 13.0psi @17:45:36.36 SS.reconfigure :inhaling 1.41ml/s @17:45:36.41 SS.seek 25ml @17:45:57.54 Stroke completed -- waiting for pressure to equalize ... @17:45:57.55 SS.reconfigure :exhale @17:45:57.59 SS.hold @17:46:16.35 Intake.select :close @17:46:16.50 SSV.dial :exhaust @17:46:17.32 Exhaust.select :open @17:46:17.44 SS.seek 0ml @17:46:26.59 Exhaust.select :close @17:46:26.70 SSV.dial CBV @17:46:26.72
CBV.dial SSV @17:46:27.56 Allowing 130s for 25.0ml of remaining 925.0ml @17:46:27.57 Intake.select :open @17:46:29.73 Intake @15.6psia, vacuum from 10.0 to 13.0psi @17:46:29.75 SS.reconfigure :inhaling 1.41ml/s @17:46:29.79 SS.seek 25ml @17:46:49.81 Vacuum too high -- waiting up to 40 seconds @17:46:49.82 SS.reconfigure :exhale @17:46:49.88 SS.hold @17:46:59.03 Intake @16.1psia, vacuum from 10.0 to 13.0psi @17:46:59.05 SS.reconfigure :inhaling 1.13ml/s @17:46:59.09 SS.seek 25ml @17:46:59.69 Stroke completed -- waiting for pressure to equalize ... @17:46:59.70 SS.reconfigure :exhale @17:46:59.75 SS.hold @17:47:09.44 Intake.select :close @17:47:09.59 SSV.dial :exhaust @17:47:10.42 Exhaust.select :open @17:47:10.54 SS.seek 0ml @17:47:19.60 Exhaust.select :close @17:47:19.71 SSV.dial CBV @17:47:19.73
CBV.dial SSV @17:47:20.56 Allowing 130s for 25.0ml of remaining 900.0ml @17:47:20.57 Intake.select :open @17:47:23.08 Intake @15.6psia, vacuum from 10.0 to 13.0psi @17:47:23.17 SS.reconfigure :inhaling 1.13ml/s @17:47:23.22 SS.seek 25ml @17:47:47.68 Stroke completed -- waiting for pressure to equalize ... @17:47:47.69 SS.reconfigure :exhale @17:47:47.73 SS.hold @17:48:06.07 Intake.select :close @17:48:06.22 SSV.dial :exhaust @17:48:07.04 Exhaust.select :open @17:48:07.15 SS.seek 0ml @17:48:16.30 Exhaust.select :close @17:48:16.41 SSV.dial CBV @17:48:16.44
CBV.dial SSV @17:48:17.27 Allowing 130s for 25.0ml of remaining 875.0ml @17:48:17.28 Intake.select :open @17:48:19.45 Intake @15.5psia, vacuum from 10.0 to 13.0psi @17:48:19.47 SS.reconfigure :inhaling 1.13ml/s @17:48:19.51 SS.seek 25ml @17:48:44.24 Stroke completed -- waiting for pressure to equalize ... @17:48:44.25 SS.reconfigure :exhale @17:48:44.29 SS.hold @17:49:02.83 Intake.select :close @17:49:02.98 SSV.dial :exhaust @17:49:03.82 Exhaust.select :open @17:49:03.93 SS.seek 0ml @17:49:12.74 Exhaust.select :close @17:49:12.85 SSV.dial CBV @17:49:12.89
CBV.dial SSV @17:49:13.69 Allowing 130s for 25.0ml of remaining 850.0ml @17:49:13.71 Intake.select :open @17:49:15.87 Intake @15.6psia, vacuum from 10.0 to 13.0psi @17:49:15.89 SS.reconfigure :inhaling 1.13ml/s @17:49:15.94 SS.seek 25ml @17:49:32.81 Vacuum too high -- waiting up to 40 seconds @17:49:32.82 SS.reconfigure :exhale @17:49:32.87 SS.hold @17:49:40.43 Intake @15.9psia, vacuum from 10.0 to 13.0psi @17:49:40.45 SS.reconfigure :inhaling 0.91ml/s @17:49:40.50 SS.seek 25ml @17:49:50.24 Stroke completed -- waiting for pressure to equalize ... @17:49:50.25 SS.reconfigure :exhale @17:49:50.29 SS.hold @17:50:04.65 Intake.select :close @17:50:04.80 SSV.dial :exhaust @17:50:05.65 Exhaust.select :open @17:50:05.76 SS.seek 0ml @17:50:15.00 Exhaust.select :close @17:50:15.11 SSV.dial CBV @17:50:15.14
CBV.dial SSV @17:50:15.97 Allowing 130s for 25.0ml of remaining 825.0ml @17:50:15.99 Intake.select :open @17:50:18.15 Intake @15.5psia, vacuum from 10.0 to 13.0psi @17:50:18.17 SS.reconfigure :inhaling 0.91ml/s @17:50:18.64 SS.seek 25ml @17:50:41.46 Vacuum too high -- waiting up to 40 seconds @17:50:41.48 SS.reconfigure :exhale @17:50:41.53 SS.hold @17:50:49.09 Intake @15.6psia, vacuum from 10.0 to 13.0psi @17:50:49.11 SS.reconfigure :inhaling 0.73ml/s @17:50:49.16 SS.seek 25ml @17:50:56.88 Stroke completed -- waiting for pressure to equalize ... @17:50:56.89 SS.reconfigure :exhale @17:50:56.93 SS.hold @17:51:11.19 Intake.select :close @17:51:11.35 SSV.dial :exhaust @17:51:12.18 Exhaust.select :open @17:51:12.29 SS.seek 0ml @17:51:21.06 Exhaust.select :close @17:51:21.17 SSV.dial CBV @17:51:21.20
CBV.dial SSV @17:51:22.04 Allowing 130s for 25.0ml of remaining 800.0ml @17:51:22.05 Intake.select :open @17:51:24.21 Intake @15.5psia, vacuum from 10.0 to 13.0psi @17:51:24.23 SS.reconfigure :inhaling 0.73ml/s @17:51:24.27 SS.seek 25ml @17:52:00.94 Stroke completed -- waiting for pressure to equalize ... @17:52:00.95 SS.reconfigure :exhale @17:52:00.99 SS.hold @17:52:21.75 Intake.select :close @17:52:21.97 SSV.dial :exhaust @17:52:22.81 Exhaust.select :open @17:52:22.92 SS.seek 0ml @17:52:32.05 Exhaust.select :close @17:52:32.16 SSV.dial CBV @17:52:32.18
CBV.dial SSV @17:52:33.03 Allowing 130s for 25.0ml of remaining 775.0ml @17:52:33.05 Intake.select :open @17:52:35.21 Intake @15.5psia, vacuum from 10.0 to 13.0psi @17:52:35.23 SS.reconfigure :inhaling 0.73ml/s @17:52:35.27 SS.seek 25ml @17:53:01.65 Vacuum too high -- waiting up to 40 seconds @17:53:01.66 SS.reconfigure :exhale @17:53:01.71 SS.hold @17:53:11.60 Intake @15.7psia, vacuum from 10.0 to 13.0psi @17:53:11.62 SS.reconfigure :inhaling 0.59ml/s @17:53:11.66 SS.seek 25ml @17:53:23.76 Stroke completed -- waiting for pressure to equalize ... @17:53:23.77 SS.reconfigure :exhale @17:53:23.82 SS.hold @17:53:40.43 Intake.select :close @17:53:40.58 SSV.dial :exhaust @17:53:41.41 Exhaust.select :open @17:53:41.52 SS.seek 0ml @17:53:50.76 Exhaust.select :close @17:53:50.87 SSV.dial CBV @17:53:50.90
CBV.dial SSV @17:53:51.74 Allowing 130s for 25.0ml of remaining 750.0ml @17:53:51.75 Intake.select :open @17:53:53.91 Intake @15.4psia, vacuum from 9.9 to 12.9psi @17:53:53.93 SS.reconfigure :inhaling 0.59ml/s @17:53:53.97 SS.seek 25ml @17:54:33.24 Vacuum too high -- waiting up to 40 seconds @17:54:33.25 SS.reconfigure :exhale @17:54:33.30 SS.hold @17:54:44.84 Intake @15.8psia, vacuum from 10.0 to 13.0psi @17:54:44.85 SS.reconfigure :inhaling 0.47ml/s @17:54:44.90 SS.seek 25ml @17:54:51.40 Stroke completed -- waiting for pressure to equalize ... @17:54:51.41 SS.reconfigure :exhale @17:54:51.46 SS.hold @17:55:07.65 Intake.select :close @17:55:07.80 SSV.dial :exhaust @17:55:08.62 Exhaust.select :open @17:55:08.74 SS.seek 0ml @17:55:17.92 Exhaust.select :close @17:55:18.03 SSV.dial CBV @17:55:18.06
CBV.dial SSV @17:55:18.87 Allowing 130s for 25.0ml of remaining 725.0ml @17:55:18.89 Intake.select :open @17:55:21.05 Intake @15.3psia, vacuum from 9.8 to 12.8psi @17:55:21.07 SS.reconfigure :inhaling 0.47ml/s @17:55:21.12 SS.seek 25ml @17:56:17.07 Stroke completed -- waiting for pressure to equalize ... @17:56:17.08 SS.reconfigure :exhale @17:56:17.13 SS.hold @17:56:43.91 Intake.select :close @17:56:44.06 SSV.dial :exhaust @17:56:44.91 Exhaust.select :open @17:56:45.03 SS.seek 0ml @17:56:53.93 Exhaust.select :close @17:56:54.04 SSV.dial CBV @17:56:54.07
CBV.dial SSV @17:56:54.90 Allowing 130s for 25.0ml of remaining 700.0ml @17:56:54.92 Intake.select :open @17:56:57.08 Intake @15.2psia, vacuum from 9.7 to 12.7psi @17:56:57.10 SS.reconfigure :inhaling 0.47ml/s @17:56:57.15 SS.seek 25ml @17:57:53.73 Stroke completed -- waiting for pressure to equalize ... @17:57:53.74 SS.reconfigure :exhale @17:57:53.78 SS.hold @17:58:26.69 Intake.select :close @17:58:26.84 SSV.dial :exhaust @17:58:27.67 Exhaust.select :open @17:58:27.79 SS.seek 0ml @17:58:36.65 Exhaust.select :close @17:58:36.76 SSV.dial CBV @17:58:36.79
CBV.dial SSV @17:58:37.64 Allowing 130s for 25.0ml of remaining 675.0ml @17:58:37.65 Intake.select :open @17:58:39.81 Intake @15.3psia, vacuum from 9.8 to 12.8psi @17:58:39.83 SS.reconfigure :inhaling 0.47ml/s @17:58:39.87 SS.seek 25ml @17:59:19.72 Vacuum too high -- waiting up to 40 seconds @17:59:19.73 SS.reconfigure :exhale @17:59:19.78 SS.hold @17:59:32.58 Intake @15.7psia, vacuum from 10.0 to 13.0psi @17:59:32.60 SS.reconfigure :inhaling 0.38ml/s @17:59:32.65 SS.seek 25ml @17:59:54.69 Stroke completed -- waiting for pressure to equalize ... @17:59:54.70 SS.reconfigure :exhale @17:59:54.75 SS.hold @18:00:23.22 Intake.select :close @18:00:23.37 SSV.dial :exhaust @18:00:24.19 Exhaust.select :open @18:00:24.31 SS.seek 0ml @18:00:33.68 Exhaust.select :close @18:00:33.79 SSV.dial CBV @18:00:33.81
CBV.dial SSV @18:00:34.65 Allowing 130s for 25.0ml of remaining 650.0ml @18:00:34.66 Intake.select :open @18:00:36.82 Intake @15.3psia, vacuum from 9.8 to 12.8psi @18:00:36.84 SS.reconfigure :inhaling 0.38ml/s @18:00:36.88 SS.seek 25ml @18:01:47.72 Stroke completed -- waiting for pressure to equalize ... @18:01:47.73 SS.reconfigure :exhale @18:01:47.78 SS.hold @18:02:26.66 Intake.select :close @18:02:26.81 SSV.dial :exhaust @18:02:27.64 Exhaust.select :open @18:02:27.75 SS.seek 0ml @18:02:37.08 Exhaust.select :close @18:02:37.19 SSV.dial CBV @18:02:37.22
CBV.dial SSV @18:02:38.05 Allowing 130s for 25.0ml of remaining 625.0ml @18:02:38.06 Intake.select :open @18:02:40.23 Intake @15.4psia, vacuum from 9.9 to 12.9psi @18:02:40.24 SS.reconfigure :inhaling 0.38ml/s @18:02:40.29 SS.seek 25ml @18:03:51.03 Stroke completed -- waiting for pressure to equalize ... @18:03:51.04 SS.reconfigure :exhale @18:03:51.09 SS.hold @18:04:31.27 SS.reconfigure :default @18:04:31.32 SS.hold @18:04:31.38 Intake.select :close @18:04:31.54 ShallowSampler::Clogged in MAIN -- Intake @15psia & Outlet @13psia won't equalize after 400.0ml @18:04:31.55 SSV.dial :exhaust @18:04:32.38 Exhaust.select :open @18:04:32.49 SS.reconfigure :exhale @18:04:32.54 SS.seek 0ml @18:04:41.94 Exhaust.select :close @18:04:42.05 Sampled 400.0ml @18:04:42.06 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @18:04:42.07 Intake.select :close @18:04:42.14 CSV.dial :waste @18:04:42.30 SSV.dial CBV,avoiding: :exhaust @18:04:42.33 CS.seek 0ml @18:04:42.35
CBV.dial SSV @18:04:42.88 CSV.dial :air @18:04:43.70 CS.seek 10ml @18:04:44.88
CTV.dial :air @18:04:45.76 delay 15 seconds @18:05:00.78 SSV.dial :spit,avoiding: :exhaust @18:05:02.24 CTV.dial CSV,avoiding: :intake @18:05:02.28
SS.seek 0ml @18:05:02.40 SSV.dial CBV,avoiding: :exhaust @18:05:11.95 SSV.dial between CBV and plug @18:05:12.47 CBV.dial between SSV and waste2 @18:05:12.88 CTV.dial CSV,avoiding: :air @18:05:12.91
CSV.dial CTV @18:05:13.45 CS.reconfigure :pressurize @18:05:13.50 CS.seek 0ml @18:05:32.14 BubblePoint: Intake @24.2psia, Outlet @0.2psia @18:05:32.15 CBV.dial SSV @18:05:32.50 SS.reconfigure :default @18:05:32.55 SS.seek 10ml @18:05:38.61 delay 15 seconds @18:05:53.73 Evacuated: Intake @23.8psia, Outlet @23.6psia @18:05:53.75 SSV.dial :spit,avoiding: :exhaust @18:05:54.90 SS.reconfigure :exhale @18:05:54.94 SS.seek 0ml @18:06:00.06 SS.reconfigure :fast @18:06:00.10 SS.seek 5ml @18:06:02.99 SSV.dial CBV,avoiding: :exhaust @18:06:03.02
CBV.dial SSV @18:06:04.49 CTV.dial :air,avoiding: :intake @18:06:06.01 CSV.dial CTV @18:06:06.05
SS.seek 10ml @18:06:07.27 CSV.dial :air @18:06:07.73 CS.reconfigure :fast @18:06:07.78 CS.seek 0ml @18:06:08.63
SSV.dial :spit,avoiding: :exhaust @18:06:10.08 SS.reconfigure :exhale @18:06:10.13 SS.seek 0ml @18:06:15.39 SS.reconfigure :fast @18:06:15.43 SS.seek 5ml @18:06:16.60 CS.stop @18:06:18.54 SSV.dial CBV,avoiding: :exhaust @18:06:18.56
CBV.dial SSV @18:06:21.06 SSV.dial :spit,avoiding: :exhaust @18:06:22.52 SS.reconfigure :default @18:06:22.57 SS.seek 0ml @18:06:26.10 SS.stop @18:06:26.14 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @18:06:26.15 Intake.select :close @18:06:26.22 CSV.dial :waste @18:06:26.36 SSV.dial CBV,avoiding: :exhaust @18:06:26.40
CBV.dial SSV @18:06:27.06 CS.seek 0ml @18:06:27.13 CSV.dial :air @18:06:27.86
CTV.dial :air @18:06:27.94 delay 15 seconds @18:06:27.97 CS.seek 10ml @18:06:42.95
SSV.dial :spit,avoiding: :exhaust @18:06:44.41 CTV.dial CSV,avoiding: :intake @18:06:44.45
SS.reconfigure :exhale @18:06:44.53 SS.seek 0ml @18:06:45.00 SSV.dial CBV,avoiding: :exhaust @18:06:55.92 SSV.dial between CBV and plug @18:06:56.44 CBV.dial between SSV and waste2 @18:06:56.86 CSV.dial between air and CTV @18:06:57.20 CS.seek 7.544ml @18:07:04.36 CTV.dial CSV,avoiding: :air @18:07:04.39
CSV.dial CTV @18:07:04.73 CS.reconfigure :pressurize @18:07:04.78 CS.seek 0ml @18:07:18.64 BubblePoint: Intake @24.1psia, Outlet @3.1psia @18:07:18.65 CBV.dial SSV @18:07:19.01 SS.reconfigure :default @18:07:19.06 SS.seek 10ml @18:07:24.72 delay 15 seconds @18:07:39.85 Evacuated: Intake @23.9psia, Outlet @23.5psia @18:07:39.86 SSV.dial :spit,avoiding: :exhaust @18:07:41.00 SS.reconfigure :exhale @18:07:41.05 SS.seek 0ml @18:07:46.54 SS.reconfigure :fast @18:07:46.59 SS.seek 5ml @18:07:49.38 SSV.dial CBV,avoiding: :exhaust @18:07:49.41
CBV.dial SSV @18:07:50.91 CTV.dial :air,avoiding: :intake @18:07:52.42 CSV.dial CTV @18:07:52.45
SS.seek 10ml @18:07:53.58 CSV.dial :air @18:07:54.07 CS.reconfigure :fast @18:07:54.12 CS.seek 0ml @18:07:55.19
SSV.dial :spit,avoiding: :exhaust @18:07:57.00 SS.reconfigure :exhale @18:07:57.12 SS.seek 0ml @18:08:01.44 CS.stop @18:08:02.32
SS.reconfigure :fast @18:08:02.37 SS.seek 5ml @18:08:05.32 SSV.dial CBV,avoiding: :exhaust @18:08:05.34
CBV.dial SSV @18:08:07.85 SSV.dial :spit,avoiding: :exhaust @18:08:09.30 SS.reconfigure :default @18:08:09.35 SS.seek 0ml @18:08:12.59 SS.stop @18:08:12.63 Lysing: Adding 2.0ml AqMeOH @18:08:12.64 CBV.dial between CSV and waste1,avoiding: :DBV @18:08:14.32 CTV.dial CSV @18:08:15.82 CSV.dial CSR @18:08:16.81 Pulling 1ml of CSR.AqMeOH @18:08:16.82 CSR.select :AqMeOH @18:08:16.93 CS.reconfigure :default @18:08:16.98 CS.seek 1ml @18:08:20.76 delay 5 seconds @18:08:25.78 CSR.select :air @18:08:25.94 CS.seek 6ml @18:08:40.32 delay 2 seconds @18:08:42.33 CSV.dial :waste2 @18:08:42.84 CS.reconfigure :fast @18:08:42.89 CS.seek 0ml @18:08:59.25 CSV.dial CSR @18:08:59.86 Pulling 2ml of CSR.AqMeOH @18:08:59.87 CSR.select :AqMeOH @18:08:59.99 CS.reconfigure :default @18:09:00.04 CS.seek 2ml @18:09:06.29 delay 5 seconds @18:09:11.30 CSR.select :air @18:09:11.47 CS.seek 5ml @18:09:20.25 delay 2 seconds @18:09:22.27 CSV.dial CTV @18:09:22.78 CS.seek 1ml @18:09:34.39 Before lysing Collection Puck Heater at 19.5C/29.9psia @18:09:34.42 Heating Puck.from 2, :sh1 in Collection Clamp to 70C for 10:00 @18:09:34.43 CH.seek 70C,8:00 @18:11:59.00 CH.hold 10:00,70C @18:11:59.20 Begun lysing Collection Puck Heater at 68C/35.8psia @18:21:59.21 Ended lysing Collection Puck Heater at 70C/35.0psia @18:21:59.23 CS.seek 0ml @18:22:02.90 CTV.dial between spare and air,avoiding: :intake @18:22:04.09 CSV.dial :air @18:22:04.58 CS.seek 5ml @18:22:19.61 CSV.dial CTV @18:22:20.10 delay 2 seconds @18:22:22.12 CSV.dial :air @18:22:22.62 delay 2 seconds @18:22:24.63 CSV.dial CTV @18:22:25.13 delay 2 seconds @18:22:27.15 CSV.dial :air @18:22:27.65 delay 2 seconds @18:22:29.67 CS.seek 0ml @18:22:44.04 Puck @80.8psia after final lysis pressurization @18:22:44.05 Recovering Lysate into Collection syringe @18:22:44.06 CSV.dial CBV @18:22:45.22 CBV.dial CSV @18:22:45.65 CS.seek 5.001ml @18:23:00.30 delay 5 seconds @18:23:05.32 CTV.dial :air @18:23:05.90 CS.seek 7.001ml @18:23:12.33 delay 5 seconds @18:23:17.35 CSV.dial :air2 @18:23:17.85 CS.seek 10ml @18:23:27.09 CSV.dial between air and CTV,avoiding: CBV @18:23:28.40 Lysing Complete @18:23:28.47 Storing sh1 puck in Collection Clamp to tube 1 @18:23:28.64 SC.reconfigure :tight @18:23:28.69 SC.seek 1 @18:23:32.24 SC.configure :limp @18:23:32.44 Elbow.reconfigure :tight @18:23:32.49 Elbow.seek 1 @18:23:34.29 Forearm.reconfigure :tight @18:23:34.34 Forearm.seek 1 + 450 counts @18:23:35.83 SE.reconfigure :pick @18:23:35.88 SE.seek :beamBreak @18:23:39.08 SE.configure :default @18:23:39.24 Elbow.reconfigure :limp @18:23:39.28 Forearm.reconfigure :loose @18:23:39.33 Forearm.seek 1 @18:23:39.58 Elbow.hold @18:23:39.64 Elbow.reconfigure :tight @18:23:39.74 SE.seek 3.15 Pucks below Top Plate @18:23:40.21 Forearm.force -20 @18:23:40.25 Forearm.seek 1 + 308 counts @18:23:40.56 Forearm.reconfigure :tight @18:23:40.61 Forearm.seek 1 + 450 counts @18:23:40.85 SE.seek 4.15 Pucks below Top Plate @18:23:41.38 Forearm.reconfigure :loose @18:23:41.42 Forearm.seek :retract @18:23:42.41 Elbow.seek CC @18:23:44.12 Elbow.reconfigure :loose @18:23:44.22 Forearm.seek CC @18:23:45.19 Hand.close @18:23:45.93 CC.seek :open @18:23:50.71 Forearm.seek :retract @18:23:51.69 Elbow.reconfigure :tight @18:23:51.74 Elbow.seek 1 @18:23:53.52 Elbow.reconfigure :limp @18:23:53.57 Forearm.seek 1 @18:23:54.61 Elbow.hold @18:23:54.67 Elbow.reconfigure :tight @18:23:54.72 Hand.open @18:23:55.98 Forearm.force -20 @18:23:56.02 Forearm.seek 1 + 312 counts @18:23:56.32 Forearm.reconfigure :tight @18:23:56.37 Forearm.seek 1 + 450 counts @18:23:56.64 SE.seek :down @18:23:57.77
Forearm.reconfigure :loose @18:23:57.86 Forearm.seek :retract @18:23:59.19 SE.stop @18:23:59.24
Tranferring DA @18:23:59.25 Beginning samplePassC @18:23:59.26 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @18:23:59.44 Elbow.seek FlushPuck::Garage @18:24:01.37 Elbow.reconfigure :loose @18:24:01.47 Forearm.seek :garage @18:24:02.52 Hand.close @18:24:03.41 Forearm.seek :retract @18:24:04.39 Elbow.reconfigure :tight @18:24:04.44 Elbow.seek CC @18:24:07.51 Elbow.reconfigure :loose @18:24:07.61 Forearm.reconfigure :tight @18:24:07.66 Forearm.seek CC @18:24:08.86 CC.seek :closed @18:24:13.89 Collection Clamp onSpring/offSpring for 15/26 samples, on/off torque 3.34 @18:24:13.97 Hand.open @18:24:14.74 Forearm.reconfigure :loose @18:24:14.79 Forearm.seek :retract @18:24:15.73 PSV.dial PSR @18:24:17.44 Pulling 1.5ml of PSR.flush @18:24:17.46 PSR.select :flush @18:24:17.58 PS.seek 1.5ml @18:24:35.00 delay 5 seconds @18:24:40.02 PSV.dial :waste1 @18:24:40.55 PS.seek 0ml @18:24:57.87 PSV.dial PSR @18:24:58.39 PSR.select :air @18:24:58.51 PS.seek 2.5ml @18:25:27.47 PRV.dial CBV,avoiding: :ab2 @18:25:27.51
CBV.dial PRV @18:25:29.47 PSV.dial :mixloop @18:25:30.66 CTV.dial :air,avoiding: :intake @18:25:30.83 PTV.dial :PRVmixing @18:25:32.35 PS.seek 0ml @18:25:59.71 PSV.dial :air @18:26:00.35 PS.seek 2.5ml @18:26:27.99 PSV.dial :mixloop @18:26:28.50 PS.seek 0ml @18:26:55.90 PSV.dial :air @18:26:56.42 PS.seek 2.5ml @18:27:24.15 PSV.dial :mixloop @18:27:24.67 PS.seek 0ml @18:27:52.25 Garaging FlushPuck in Collection Clamp @18:27:52.31 Elbow.reconfigure :tight @18:27:52.36 Elbow.seek CC @18:27:52.65 Elbow.reconfigure :loose @18:27:52.75 Forearm.seek CC @18:27:53.79 Hand.close @18:27:54.55 CC.seek :open @18:27:59.23 Forearm.seek :retract @18:28:00.20 Elbow.reconfigure :tight @18:28:00.68 Elbow.seek :garage @18:28:03.66 Elbow.reconfigure :loose @18:28:03.77 Forearm.reconfigure :tight @18:28:03.81 Forearm.seek :garage @18:28:04.87 Hand.open @18:28:05.60 Forearm.reconfigure :loose @18:28:05.65 Forearm.seek :retract @18:28:06.65 PSV.dial :mixloop @18:28:06.73 PTV.dial :PRVmixing @18:28:06.81 PRV.dial CBV @18:28:06.85
CBV.dial PRV @18:28:07.08 CTV.dial :air,avoiding: :intake @18:28:07.10 Loading lyfil puck from tube 2 into Collection Clamp @18:28:07.33 SC.reconfigure :tight @18:28:07.37 SC.seek 2 @18:28:10.98 SC.configure :limp @18:28:11.19 Elbow.reconfigure :tight @18:28:11.24 Elbow.seek 2 @18:28:13.21 Forearm.reconfigure :tight @18:28:13.26 Forearm.seek 2 + 450 counts @18:28:14.26 SE.reconfigure :pick @18:28:14.31 SE.seek :beamBreak @18:28:15.68 SE.configure :default @18:28:15.84 Elbow.seek 2 @18:28:16.03 Forearm.seek 2 + 300 counts @18:28:16.33 Forearm.reconfigure :loose @18:28:16.37 Hand.close @18:28:17.06 SE.seek :down @18:28:17.26
Forearm.seek :retract @18:28:18.02 SE.stop @18:28:18.69
Elbow.seek CC @18:28:20.32 Elbow.reconfigure :loose @18:28:20.42 Forearm.reconfigure :tight @18:28:20.47 Forearm.seek CC @18:28:21.71 CC.seek :closed @18:28:26.30 Collection Clamp onSpring/offSpring for 15/26 samples, on/off torque 3.18 @18:28:26.31 Hand.open @18:28:27.12 Forearm.reconfigure :loose @18:28:27.17 Forearm.seek :retract @18:28:28.16 CTV.dial CSV,avoiding: :intake @18:28:29.74 CSV.dial CTV @18:28:30.08 PS.seek 2.5ml @18:28:59.27 CS.seek 4.5ml @18:29:15.30 delay 8 seconds @18:29:23.32 PSV.dial :air @18:29:23.84 delay 2 seconds @18:29:25.85 PSV.dial :waste2 @18:29:26.69 PS.seek 0ml @18:29:54.77 CBV.dial between PRV and waste1 @18:29:55.11 CSV.dial :air @18:29:55.61 CS.seek 10ml @18:30:12.01 CSV.dial CTV @18:30:12.52 CS.seek 0ml @18:30:40.61 CBV.dial :waste1 @18:30:40.97 delay 5 seconds @18:30:45.99 CTV.dial :air,avoiding: :intake @18:30:47.60 Storing lyfil puck in Collection Clamp to tube 1 @18:30:48.22 SC.reconfigure :tight @18:30:48.26 SC.seek 1 @18:30:51.88 SC.configure :limp @18:30:52.10 Elbow.reconfigure :tight @18:30:52.14 Elbow.seek 1 @18:30:53.96 Forearm.reconfigure :tight @18:30:54.01 Forearm.seek 1 + 450 counts @18:30:55.06 SE.reconfigure :pick @18:30:55.11 SE.seek :beamBreak @18:30:58.29 SE.configure :default @18:30:58.44 Elbow.reconfigure :limp @18:30:58.49 Forearm.reconfigure :loose @18:30:58.54 Forearm.seek 1 @18:30:58.78 Elbow.hold @18:30:58.84 Elbow.reconfigure :tight @18:30:58.94 SE.seek 4.15 Pucks below Top Plate @18:30:59.40 Forearm.force -20 @18:30:59.44 Forearm.seek 1 + 308 counts @18:30:59.73 Forearm.reconfigure :tight @18:30:59.78 Forearm.seek 1 + 450 counts @18:30:59.98 SE.seek 5.15 Pucks below Top Plate @18:31:00.62 Forearm.reconfigure :loose @18:31:00.67 Forearm.seek :retract @18:31:01.59 Elbow.seek CC @18:31:03.28 Elbow.reconfigure :loose @18:31:03.38 Forearm.seek CC @18:31:04.37 Hand.close @18:31:05.04 CC.seek :open @18:31:10.27 Forearm.seek :retract @18:31:11.25 Elbow.reconfigure :tight @18:31:11.30 Elbow.seek 1 @18:31:13.11 Elbow.reconfigure :limp @18:31:13.16 Forearm.seek 1 @18:31:14.12 Elbow.hold @18:31:14.19 Elbow.reconfigure :tight @18:31:14.23 Hand.open @18:31:15.04 Forearm.force -20 @18:31:15.08 Forearm.seek 1 + 309 counts @18:31:15.38 Forearm.reconfigure :tight @18:31:15.43 Forearm.seek 1 + 450 counts @18:31:15.73 SE.seek :down @18:31:16.87
Forearm.reconfigure :loose @18:31:16.91 Forearm.seek :retract @18:31:18.22 SE.stop @18:31:18.26
PSV.dial :air @18:31:19.25 PS.seek 2.5ml @18:31:47.61 delay 1 second @18:31:48.63 PSV.dial :mixloop @18:31:49.15 delay 1 second @18:31:50.16 PSV.dial :air @18:31:50.68 delay 1 second @18:31:51.69 PSV.dial :mixloop @18:31:52.21 delay 1 second @18:31:53.22 PSV.dial :air @18:31:53.85 delay 1 second @18:31:54.87 PS.seek 0ml @18:32:23.40 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @18:32:23.58 Elbow.seek FlushPuck::Garage @18:32:25.52 Elbow.reconfigure :loose @18:32:26.04 Forearm.seek :garage @18:32:27.07 Hand.close @18:32:27.91 Forearm.seek :retract @18:32:28.93 Elbow.reconfigure :tight @18:32:28.98 Elbow.seek PC @18:32:30.60 Elbow.reconfigure :loose @18:32:30.70 Forearm.reconfigure :tight @18:32:30.74 Forearm.seek PC @18:32:32.01 PC.seek :closed @18:32:36.79 Processing Clamp onSpring/offSpring for 16/25 samples, on/off torque 2.80 @18:32:36.86 Hand.open @18:32:37.60 Forearm.reconfigure :loose @18:32:37.65 Forearm.seek :retract @18:32:38.66 PRV.dial :air @18:32:39.16 PSV.dial :mixloop @18:32:39.72 PS.seek 0.3ml @18:32:43.99 PSV.dial PSR @18:32:45.19 Pulling 0.4ml of PSR.air @18:32:45.20 PSR.select :air @18:32:45.22 PS.seek 0.7ml @18:32:50.43 delay 3 seconds @18:32:53.45 PSR.select :air @18:32:53.47 PS.seek 2.5ml @18:33:13.83 delay 3 seconds @18:33:16.85 PSV.dial :mixloop @18:33:18.05 PTV.dial :mixing @18:33:18.92 PBV.dial :waste2 @18:33:20.01 PS.seek 0ml @18:33:48.74 Garaging FlushPuck in Processing Clamp @18:33:48.81 Elbow.reconfigure :tight @18:33:48.85 Elbow.seek PC @18:33:49.41 Elbow.reconfigure :loose @18:33:49.51 Forearm.seek PC @18:33:50.52 Hand.close @18:33:51.29 PC.seek :open @18:33:56.05 Forearm.seek :retract @18:33:57.06 Elbow.reconfigure :tight @18:33:57.11 Elbow.seek :garage @18:33:58.41 Elbow.reconfigure :loose @18:33:58.51 Forearm.reconfigure :tight @18:33:58.55 Forearm.seek :garage @18:33:59.63 Hand.open @18:34:00.47 Forearm.reconfigure :loose @18:34:00.52 Forearm.seek :retract @18:34:01.51 Loading da puck from tube 2 into Processing Clamp @18:34:01.74 SC.reconfigure :tight @18:34:01.79 SC.seek 2 @18:34:05.36 SC.configure :limp @18:34:05.57 Elbow.reconfigure :tight @18:34:05.61 Elbow.seek 2 @18:34:07.50 Forearm.reconfigure :tight @18:34:07.55 Forearm.seek 2 + 450 counts @18:34:08.58 SE.reconfigure :pick @18:34:08.64 SE.seek :beamBreak @18:34:10.19 SE.configure :default @18:34:10.35 Elbow.seek 2 @18:34:10.53 Forearm.seek 2 + 300 counts @18:34:10.76 Forearm.reconfigure :loose @18:34:10.80 Hand.close @18:34:11.55 SE.seek :down @18:34:12.19
Forearm.seek :retract @18:34:13.02 SE.stop @18:34:13.14
Elbow.seek PC @18:34:15.80 Elbow.reconfigure :loose @18:34:15.90 Forearm.reconfigure :tight @18:34:15.94 Forearm.seek PC @18:34:17.15 PC.seek :closed @18:34:21.85 Processing Clamp onSpring/offSpring for 16/26 samples, on/off torque 2.58 @18:34:21.86 Hand.open @18:34:22.60 Forearm.reconfigure :loose @18:34:22.64 Forearm.seek :retract @18:34:23.59 Heating Puck.from 2, :da in Processing Clamp to 28C for 2:00:00 @18:34:23.60 PH.seek 28C,5:00 @18:34:56.80 PH.hold 2:00:00,28C @18:34:56.85 delay 1:30 @18:36:26.90 PSR.select :air @18:36:26.92 PSV.dial PSR @18:36:28.12 Pulling 0.5ml of PSR.wash @18:36:28.13 PSR.select :wash @18:36:28.25 PS.seek 0.5ml @18:36:34.80 delay 5 seconds @18:36:39.81 PSR.select :air @18:36:39.94 PS.seek 2ml @18:36:57.54 PTV.dial :puck @18:36:58.37 PSV.dial PTV @18:36:59.34 PBV.dial :waste1 @18:37:00.92 PS.seek 1.5ml @18:37:07.11 delay 3 seconds @18:37:10.13 PBV.dial between PSV and waste1 @18:37:10.52 PS.seek 0ml @18:37:27.70 delay 5:00 @18:42:27.72 recoverPSRreagent08 @18:42:27.73 PTV.dial :puck @18:42:27.80 PBV.dial :waste1 @18:42:28.21 delay 30 seconds @18:42:58.24 PBV.dial between PSV and waste1 @18:42:58.65 PSR.select :air @18:42:58.66 PSV.dial PSR @18:42:59.51 PS.reconfigure :fast @18:42:59.56 PS.seek 2.5ml @18:43:27.69 PSV.dial PTV @18:43:28.57 delay 5 seconds @18:43:33.59 PSV.dial PSR @18:43:34.44 delay 5 seconds @18:43:39.46 PSV.dial PTV @18:43:40.32 delay 5 seconds @18:43:45.34 PSV.dial :air @18:43:46.21 PS.seek 0ml @18:44:14.32 PTV.dial :PRVmixing,via: PRV @18:44:15.82 PSV.dial :mixloop @18:44:16.33 PRV.dial :ab1 @18:44:17.18 PS.reconfigure :slow @18:44:17.23 PS.seek 0.15ml @18:44:20.75 delay 2 seconds @18:44:22.77 CBV.dial PRV @18:44:22.80
PRV.dial CBV @18:44:23.41 PS.seek 0.35ml @18:44:28.04 delay 2 seconds @18:44:30.42 CBV.dial between PRV and PSV @18:44:30.83 PRV.dial :air @18:44:31.32 PS.seek 0.9ml @18:44:43.13 PRV.dial :waste2 @18:44:43.96 PS.seek 0.5ml @18:44:52.63 addPMLReagent08 @18:44:52.64 PSV.dial :air @18:44:53.26 PS.reconfigure :default @18:44:53.31 PS.seek 2ml @18:45:10.16 PSV.dial :PML @18:45:10.77 PTV.dial :mixing @18:45:11.61 PBV.dial :waste1 @18:45:12.07 PS.seek 1.85ml @18:45:14.41 PBV.dial between PSV and waste1 @18:45:14.81 PS.seek 0ml @18:45:35.32 delay 2 seconds @18:45:37.33 Beginning samplePassC @18:45:37.35 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @18:45:37.53 Elbow.reconfigure :tight @18:45:37.58 Elbow.seek FlushPuck::Garage @18:45:38.94 Elbow.reconfigure :loose @18:45:39.04 Forearm.seek :garage @18:45:40.08 Hand.close @18:45:40.91 Forearm.seek :retract @18:45:41.92 Elbow.reconfigure :tight @18:45:41.97 Elbow.seek CC @18:45:44.99 Elbow.reconfigure :loose @18:45:45.09 Forearm.reconfigure :tight @18:45:45.14 Forearm.seek CC @18:45:46.44 CC.seek :closed @18:45:51.07 Collection Clamp onSpring/offSpring for 15/26 samples, on/off torque 3.90 @18:45:51.13 Hand.open @18:45:51.98 Forearm.reconfigure :loose @18:45:52.02 Forearm.seek :retract @18:45:53.03 PSV.dial PSR @18:45:54.36 Pulling 1.5ml of PSR.flush @18:45:54.37 PSR.select :flush @18:45:54.49 PS.seek 1.5ml @18:46:11.63 delay 5 seconds @18:46:16.65 PSV.dial :waste1 @18:46:17.18 PS.seek 0ml @18:46:34.00 PSV.dial PSR @18:46:34.52 PSR.select :air @18:46:34.63 PS.seek 2.5ml @18:47:02.60 PRV.dial CBV,avoiding: :ab2 @18:47:02.63
CBV.dial PRV @18:47:04.45 PSV.dial :mixloop @18:47:05.64 CTV.dial :air,avoiding: :intake @18:47:05.72 PTV.dial :PRVmixing @18:47:06.58 PS.seek 0ml @18:47:34.66 PSV.dial :air @18:47:35.18 PS.seek 2.5ml @18:48:03.50 PSV.dial :mixloop @18:48:04.15 PS.seek 0ml @18:48:31.94 PSV.dial :air @18:48:32.44 PS.seek 2.5ml @18:49:01.15 PSV.dial :mixloop @18:49:01.68 PS.seek 0ml @18:49:29.78 Garaging FlushPuck in Collection Clamp @18:49:29.85 Elbow.reconfigure :tight @18:49:29.90 Elbow.seek CC @18:49:30.17 Elbow.reconfigure :loose @18:49:30.27 Forearm.seek CC @18:49:31.32 Hand.close @18:49:32.03 CC.seek :open @18:49:36.67 Forearm.seek :retract @18:49:38.12 Elbow.reconfigure :tight @18:49:38.17 Elbow.seek :garage @18:49:41.21 Elbow.reconfigure :loose @18:49:41.32 Forearm.reconfigure :tight @18:49:41.36 Forearm.seek :garage @18:49:42.31 Hand.open @18:49:43.09 Forearm.reconfigure :loose @18:49:43.13 Forearm.seek :retract @18:49:44.08 delayUntil "18:53:37.32" @18:49:44.10 Whole Cell Archive sampling 1000ml @18:49:44.12 Loading wcr puck from tube 2 into Collection Clamp @18:49:44.59 Elbow.reconfigure :tight @18:49:44.64 Elbow.seek 2 @18:49:46.58 Forearm.reconfigure :tight @18:49:46.63 Forearm.seek 2 + 450 counts @18:49:47.64 SE.reconfigure :pick @18:49:47.70 SE.seek :beamBreak @18:49:49.23 SE.configure :default @18:49:49.39 Elbow.seek 2 @18:49:49.58 Forearm.seek 2 + 300 counts @18:49:49.78 Forearm.reconfigure :loose @18:49:49.82 Hand.close @18:49:50.54 SE.seek :down @18:49:50.74 Forearm.seek :retract @18:49:51.64 SE.stop @18:49:51.71 Elbow.seek CC @18:49:53.80 Elbow.reconfigure :loose @18:49:53.91 Forearm.reconfigure :tight @18:49:53.96 Forearm.seek CC @18:49:55.18 CC.seek :closed @18:49:59.80 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 3.26 @18:49:59.82 Hand.open @18:50:00.59 Forearm.reconfigure :loose @18:50:00.64 Forearm.seek :retract @18:50:01.62 collectSample 1000ml w/bubble point of 35psi @18:50:01.64 Priming 7ml @18:50:01.65 Intake.select :close @18:50:01.66 Exhaust.select :close @18:50:01.67 SS.hold @18:50:01.88 Calibrating pressure sensors @18:50:01.90 CTV.dial :air @18:50:01.98 SSV.dial :spit,avoiding: :exhaust @18:50:02.00 SS.reconfigure :fast @18:50:02.05 SS.seek 5ml @18:50:05.07 SSV.dial CBV @18:50:05.10 CBV.dial SSV @18:50:06.68 delay 3.8 seconds @18:50:10.62 Sensor limits: Intake max 112.6psia; Outlet max 106.6psia @18:50:10.64 SSV.dial :spit @18:50:12.14 SS.seek 0ml @18:50:14.82 SSV.dial CBV @18:50:16.35 CTV.dial :intake @18:50:17.23 SSV.dial CBV @18:50:17.26 CBV.dial SSV @18:50:17.48 Allowing 40s for 7.0ml of remaining 7.0ml @18:50:17.49 Intake.select :open @18:50:19.65 Intake @14.4psia, vacuum from 8.9 to 11.9psi @18:50:20.11 SS.reconfigure :inhaling 2.20ml/s @18:50:20.16 SS.seek 7ml @18:50:27.60 Stroke completed -- waiting for pressure to equalize ... @18:50:27.61 SS.reconfigure :exhale @18:50:27.66 SS.hold @18:50:38.83 Intake.select :close @18:50:38.99 SSV.dial :exhaust @18:50:39.85 Exhaust.select :open @18:50:39.97 SS.seek 0ml @18:50:44.19 Exhaust.select :close @18:50:44.31 Sampling 1000.0ml @18:50:44.32 Intake.select :close @18:50:44.33 Exhaust.select :close @18:50:44.34 SS.reconfigure :default @18:50:44.39 SS.hold @18:50:44.59 CTV.dial :intake @18:50:44.76 SSV.dial CBV @18:50:44.79 CBV.dial SSV @18:50:46.09 Allowing 130s for 25.0ml of remaining 1000.0ml @18:50:46.10 Intake.select :open @18:50:48.26 Intake @15.3psia, vacuum from 9.8 to 12.8psi @18:50:48.28 SS.reconfigure :inhaling 2.20ml/s @18:50:48.33 SS.seek 25ml @18:51:02.42 Vacuum too high -- waiting up to 40 seconds @18:51:02.44 SS.reconfigure :exhale @18:51:02.49 SS.hold @18:51:12.69 Intake @17.9psia, vacuum from 10.0 to 13.0psi @18:51:12.71 SS.reconfigure :inhaling 1.76ml/s @18:51:12.76 SS.seek 25ml @18:51:15.68 Stroke completed -- waiting for pressure to equalize ... @18:51:15.69 SS.reconfigure :exhale @18:51:15.73 SS.hold @18:51:34.43 Intake.select :close @18:51:34.69 SSV.dial :exhaust @18:51:35.55 Exhaust.select :open @18:51:35.67 SS.seek 0ml @18:51:44.82 Exhaust.select :close @18:51:44.93 SSV.dial CBV @18:51:44.95 CBV.dial SSV @18:51:45.82 Allowing 130s for 25.0ml of remaining 975.0ml @18:51:45.83 Intake.select :open @18:51:48.00 Intake @15.5psia, vacuum from 10.0 to 13.0psi @18:51:48.02 SS.reconfigure :inhaling 1.76ml/s @18:51:48.07 SS.seek 25ml @18:52:05.28 Stroke completed -- waiting for pressure to equalize ... @18:52:05.30 SS.reconfigure :exhale @18:52:05.34 SS.hold @18:52:34.23 Intake.select :close @18:52:34.39 SSV.dial :exhaust @18:52:35.26 Exhaust.select :open @18:52:35.37 SS.seek 0ml @18:52:44.33 Exhaust.select :close @18:52:44.89 SSV.dial CBV @18:52:44.92 CBV.dial SSV @18:52:45.79 Allowing 130s for 25.0ml of remaining 950.0ml @18:52:45.80 Intake.select :open @18:52:47.96 Intake @15.6psia, vacuum from 10.0 to 13.0psi @18:52:47.98 SS.reconfigure :inhaling 1.76ml/s @18:52:48.03 SS.seek 25ml @18:53:01.64 Vacuum too high -- waiting up to 40 seconds @18:53:01.66 SS.reconfigure :exhale @18:53:01.71 SS.hold @18:53:10.56 Intake @16.0psia, vacuum from 10.0 to 13.0psi @18:53:10.58 SS.reconfigure :inhaling 1.41ml/s @18:53:10.63 SS.seek 25ml @18:53:16.20 Stroke completed -- waiting for pressure to equalize ... @18:53:16.22 SS.reconfigure :exhale @18:53:16.26 SS.hold @18:53:37.33 recoverPRVreagent08 @18:53:37.34 PTV.dial :mixing @18:53:38.20 PBV.dial :waste1 @18:53:38.60 delay 30 seconds @18:53:39.14 Intake.select :close @18:53:39.29 SSV.dial :exhaust @18:53:40.14 Exhaust.select :open @18:53:40.26 SS.seek 0ml @18:53:49.27 Exhaust.select :close @18:53:49.38 SSV.dial CBV @18:53:49.40 CBV.dial SSV @18:53:50.27 Allowing 130s for 25.0ml of remaining 925.0ml @18:53:50.28 Intake.select :open @18:53:52.45 Intake @15.5psia, vacuum from 10.0 to 13.0psi @18:53:52.47 SS.reconfigure :inhaling 1.41ml/s @18:53:52.52 SS.seek 25ml @18:54:08.62 PBV.dial between PSV and waste1 @18:54:09.07 PSV.dial :air @18:54:09.72 PS.reconfigure :fast @18:54:09.77 PS.seek 2.5ml @18:54:12.10 Vacuum too high -- waiting up to 40 seconds @18:54:12.12 SS.reconfigure :exhale @18:54:12.18 SS.hold @18:54:23.52 Intake @16.8psia, vacuum from 10.0 to 13.0psi @18:54:23.54 SS.reconfigure :inhaling 1.13ml/s @18:54:23.59 SS.seek 25ml @18:54:26.11 Stroke completed -- waiting for pressure to equalize ... @18:54:26.12 SS.reconfigure :exhale @18:54:26.17 SS.hold @18:54:38.58 PSV.dial :mixloop @18:54:39.04 Intake.select :close @18:54:39.11 delay 2 seconds @18:54:39.21 SSV.dial :exhaust @18:54:40.07 Exhaust.select :open @18:54:40.18 SS.seek 0ml @18:54:41.13 PSV.dial :air @18:54:41.65 delay 2 seconds @18:54:43.67 PSV.dial :mixloop @18:54:44.18 delay 2 seconds @18:54:46.20 PSV.dial :air @18:54:46.72 PS.seek 0ml @18:54:49.60 Exhaust.select :close @18:54:49.71 SSV.dial CBV @18:54:49.75 CBV.dial SSV @18:54:50.60 Allowing 130s for 25.0ml of remaining 900.0ml @18:54:50.61 Intake.select :open @18:54:52.77 Intake @15.6psia, vacuum from 10.0 to 13.0psi @18:54:52.79 SS.reconfigure :inhaling 1.13ml/s @18:54:52.84 SS.seek 25ml @18:55:14.96 washCycleDA #1 of 2 @18:55:14.97 PSR.select :air @18:55:14.99 PSV.dial PSR @18:55:16.52 Pulling 0.5ml of PSR.wash @18:55:16.53 PSR.select :wash @18:55:16.65 PS.reconfigure :default @18:55:16.72 Stroke completed -- waiting for pressure to equalize ... @18:55:16.74 PS.seek 0.5ml @18:55:16.76 SS.reconfigure :exhale @18:55:17.30 SS.hold @18:55:23.66 delay 5 seconds @18:55:28.68 PSR.select :air @18:55:28.80 PS.seek 2ml @18:55:39.58 Intake.select :close @18:55:39.74 SSV.dial :exhaust @18:55:40.60 Exhaust.select :open @18:55:41.16 SS.seek 0ml @18:55:46.24 PTV.dial :puck @18:55:47.08 PSV.dial PTV @18:55:47.94 PBV.dial :waste1 @18:55:48.41 PS.seek 1.5ml @18:55:50.45 Exhaust.select :close @18:55:50.57 SSV.dial CBV @18:55:50.61 CBV.dial SSV @18:55:51.46 Allowing 130s for 25.0ml of remaining 875.0ml @18:55:51.48 Intake.select :open @18:55:53.64 Intake @15.6psia, vacuum from 10.0 to 13.0psi @18:55:53.66 SS.reconfigure :inhaling 1.13ml/s @18:55:53.71 SS.seek 25ml @18:55:54.55 delay 3 seconds @18:55:57.57 PBV.dial between PSV and waste1 @18:55:57.98 PS.seek 0ml @18:56:12.51 Vacuum too high -- waiting up to 40 seconds @18:56:12.52 SS.reconfigure :exhale @18:56:12.58 SS.hold @18:56:15.11 delay 2:00 @18:56:21.24 Intake @15.3psia, vacuum from 9.8 to 12.8psi @18:56:21.26 SS.reconfigure :inhaling 0.91ml/s @18:56:21.31 SS.seek 25ml @18:56:29.29 Stroke completed -- waiting for pressure to equalize ... @18:56:29.31 SS.reconfigure :exhale @18:56:29.36 SS.hold @18:56:43.74 Intake.select :close @18:56:43.89 SSV.dial :exhaust @18:56:44.78 Exhaust.select :open @18:56:44.90 SS.seek 0ml @18:56:54.22 Exhaust.select :close @18:56:54.33 SSV.dial CBV @18:56:54.35 CBV.dial SSV @18:56:55.23 Allowing 130s for 25.0ml of remaining 850.0ml @18:56:55.25 Intake.select :open @18:56:57.42 Intake @15.5psia, vacuum from 10.0 to 13.0psi @18:56:57.44 SS.reconfigure :inhaling 0.91ml/s @18:56:57.93 SS.seek 25ml @18:57:27.72 Stroke completed -- waiting for pressure to equalize ... @18:57:27.73 SS.reconfigure :exhale @18:57:27.78 SS.hold @18:57:50.23 Intake.select :close @18:57:50.38 SSV.dial :exhaust @18:57:51.25 Exhaust.select :open @18:57:51.76 SS.seek 0ml @18:58:01.19 Exhaust.select :close @18:58:01.30 SSV.dial CBV @18:58:01.34 CBV.dial SSV @18:58:02.20 Allowing 130s for 25.0ml of remaining 825.0ml @18:58:02.22 Intake.select :open @18:58:04.38 Intake @15.5psia, vacuum from 10.0 to 13.0psi @18:58:04.40 SS.reconfigure :inhaling 0.91ml/s @18:58:04.45 SS.seek 25ml @18:58:15.13 recoverPSRreagent08 @18:58:15.15 PTV.dial :puck @18:58:15.22 PBV.dial :waste1 @18:58:15.63 delay 30 seconds @18:58:29.91 Vacuum too high -- waiting up to 40 seconds @18:58:29.93 SS.reconfigure :exhale @18:58:29.98 SS.hold @18:58:41.25 Intake @15.5psia, vacuum from 10.0 to 13.0psi @18:58:41.27 SS.reconfigure :inhaling 0.73ml/s @18:58:41.31 SS.seek 25ml @18:58:45.65 PBV.dial between PSV and waste1 @18:58:46.05 PSR.select :air @18:58:46.45 PSV.dial PSR @18:58:46.93 Stroke completed -- waiting for pressure to equalize ... @18:58:46.95 SS.reconfigure :exhale @18:58:46.99 SS.hold @18:58:47.40 PS.reconfigure :fast @18:58:47.45 PS.seek 2.5ml @18:58:59.67 Intake.select :close @18:58:59.83 SSV.dial :exhaust @18:59:00.70 Exhaust.select :open @18:59:00.82 SS.seek 0ml @18:59:10.14 Exhaust.select :close @18:59:10.25 SSV.dial CBV @18:59:10.28 CBV.dial SSV @18:59:11.14 Allowing 130s for 25.0ml of remaining 800.0ml @18:59:11.15 Intake.select :open @18:59:13.31 Intake @15.4psia, vacuum from 9.9 to 12.9psi @18:59:13.33 SS.reconfigure :inhaling 0.73ml/s @18:59:13.83 SS.seek 25ml @18:59:16.17 PSV.dial PTV @18:59:17.05 delay 5 seconds @18:59:22.07 PSV.dial PSR @18:59:22.94 delay 5 seconds @18:59:27.96 PSV.dial PTV @18:59:28.86 delay 5 seconds @18:59:33.88 PSV.dial :air @18:59:34.76 PS.seek 0ml @18:59:49.82 Stroke completed -- waiting for pressure to equalize ... @18:59:49.84 SS.reconfigure :exhale @18:59:49.88 SS.hold @19:00:03.08 washCycleDA #2 of 2 @19:00:03.09 PSR.select :air @19:00:03.10 PSV.dial PSR @19:00:04.64 Pulling 0.5ml of PSR.wash @19:00:04.65 PSR.select :wash @19:00:04.77 PS.reconfigure :default @19:00:04.81 PS.seek 0.5ml @19:00:11.30 delay 5 seconds @19:00:16.36 PSR.select :air @19:00:16.49 Intake.select :close @19:00:16.55 PS.seek 2ml @19:00:16.66 SSV.dial :exhaust @19:00:17.51 Exhaust.select :open @19:00:17.63 SS.seek 0ml @19:00:26.60 Exhaust.select :close @19:00:26.72 SSV.dial CBV @19:00:26.75 CBV.dial SSV @19:00:27.62 Allowing 130s for 25.0ml of remaining 775.0ml @19:00:27.64 Intake.select :open @19:00:29.80 Intake @15.3psia, vacuum from 9.8 to 12.8psi @19:00:29.82 SS.reconfigure :inhaling 0.73ml/s @19:00:29.87 SS.seek 25ml @19:00:33.86 PTV.dial :puck @19:00:33.94 PSV.dial PTV @19:00:34.91 PBV.dial :waste1 @19:00:35.37 PS.seek 1.5ml @19:00:41.42 delay 3 seconds @19:00:44.44 PBV.dial between PSV and waste1 @19:00:44.85 PS.seek 0ml @19:00:56.50 Vacuum too high -- waiting up to 40 seconds @19:00:56.52 SS.reconfigure :exhale @19:00:56.57 SS.hold @19:01:01.73 delay 2:00 @19:01:09.39 Intake @15.6psia, vacuum from 10.0 to 13.0psi @19:01:09.41 SS.reconfigure :inhaling 0.59ml/s @19:01:09.46 SS.seek 25ml @19:01:22.85 Stroke completed -- waiting for pressure to equalize ... @19:01:22.86 SS.reconfigure :exhale @19:01:22.91 SS.hold @19:01:41.32 Intake.select :close @19:01:41.48 SSV.dial :exhaust @19:01:42.34 Exhaust.select :open @19:01:42.45 SS.seek 0ml @19:01:51.90 Exhaust.select :close @19:01:52.01 SSV.dial CBV @19:01:52.05 CBV.dial SSV @19:01:52.90 Allowing 130s for 25.0ml of remaining 750.0ml @19:01:52.92 Intake.select :open @19:01:55.08 Intake @15.2psia, vacuum from 9.7 to 12.7psi @19:01:55.10 SS.reconfigure :inhaling 0.59ml/s @19:01:55.15 SS.seek 25ml @19:02:38.09 Vacuum too high -- waiting up to 40 seconds @19:02:38.11 SS.reconfigure :exhale @19:02:38.16 SS.hold @19:02:50.95 Intake @14.8psia, vacuum from 9.3 to 12.3psi @19:02:50.97 SS.reconfigure :inhaling 0.47ml/s @19:02:51.02 SS.seek 25ml @19:02:52.93 Stroke completed -- waiting for pressure to equalize ... @19:02:52.94 SS.reconfigure :exhale @19:02:52.99 SS.hold @19:03:01.75 recoverPSRreagent08 @19:03:01.76 PTV.dial :puck @19:03:01.84 PBV.dial :waste1 @19:03:02.25 delay 30 seconds @19:03:09.33 Intake.select :close @19:03:09.48 SSV.dial :exhaust @19:03:10.35 Exhaust.select :open @19:03:10.47 SS.seek 0ml @19:03:19.94 Exhaust.select :close @19:03:20.06 SSV.dial CBV @19:03:20.10 CBV.dial SSV @19:03:20.95 Allowing 130s for 25.0ml of remaining 725.0ml @19:03:20.96 Intake.select :open @19:03:23.12 Intake @15.2psia, vacuum from 9.7 to 12.7psi @19:03:23.14 SS.reconfigure :inhaling 0.47ml/s @19:03:23.19 SS.seek 25ml @19:03:32.27 PBV.dial between PSV and waste1 @19:03:32.68 PSR.select :air @19:03:32.70 PSV.dial PSR @19:03:33.55 PS.reconfigure :fast @19:03:33.60 PS.seek 2.5ml @19:04:01.92 PSV.dial PTV @19:04:02.80 delay 5 seconds @19:04:07.82 PSV.dial PSR @19:04:08.69 delay 5 seconds @19:04:13.71 PSV.dial PTV @19:04:14.57 delay 5 seconds @19:04:18.98 Stroke completed -- waiting for pressure to equalize ... @19:04:18.99 SS.reconfigure :exhale @19:04:19.04 SS.hold @19:04:19.64 PSV.dial :air @19:04:20.52 PS.seek 0ml @19:04:47.65 Intake.select :close @19:04:47.80 SSV.dial :exhaust @19:04:48.60 getPMLReagent08 @19:04:48.62 PRV.dial :air @19:04:48.68 Exhaust.select :open @19:04:48.80 SS.seek 0ml @19:04:49.14 delay 5 seconds @19:04:54.16 PTV.dial :PRVmixing @19:04:55.67 delay 5 seconds @19:04:58.25 Exhaust.select :close @19:04:58.37 SSV.dial CBV @19:04:58.41 CBV.dial SSV @19:04:59.28 Allowing 130s for 25.0ml of remaining 700.0ml @19:04:59.29 Intake.select :open @19:05:00.68 PRV.dial :ab2 @19:05:01.20 PTV.dial :PRVmixing @19:05:01.21 PSV.dial :mixloop @19:05:01.45 Intake @15.2psia, vacuum from 9.7 to 12.7psi @19:05:01.47 SS.reconfigure :inhaling 0.47ml/s @19:05:01.52 SS.seek 25ml @19:05:01.75 PS.reconfigure :default @19:05:01.80 PS.seek 0.3ml @19:05:06.35 delay 2 seconds @19:05:08.37 PRV.dial :air @19:05:08.96 PS.seek 0.55ml @19:05:12.45 addPMLReagent08 @19:05:12.46 PSV.dial :air @19:05:12.98 PS.seek 2ml @19:05:29.76 PSV.dial :PML @19:05:30.29 PTV.dial :mixing @19:05:31.17 PBV.dial :waste1 @19:05:31.63 PS.seek 1.85ml @19:05:34.10 PBV.dial between PSV and waste1 @19:05:34.52 PS.seek 0ml @19:05:55.57 delay 2 seconds @19:05:57.30 Stroke completed -- waiting for pressure to equalize ... @19:05:57.31 SS.reconfigure :exhale @19:05:57.36 SS.hold @19:05:57.59 delay 8:00 @19:06:34.16 Intake.select :close @19:06:34.31 SSV.dial :exhaust @19:06:35.19 Exhaust.select :open @19:06:35.31 SS.seek 0ml @19:06:44.64 Exhaust.select :close @19:06:44.76 SSV.dial CBV @19:06:44.79 CBV.dial SSV @19:06:45.65 Allowing 130s for 25.0ml of remaining 675.0ml @19:06:45.66 Intake.select :open @19:06:47.83 Intake @15.1psia, vacuum from 9.6 to 12.6psi @19:06:47.85 SS.reconfigure :inhaling 0.47ml/s @19:06:47.90 SS.seek 25ml @19:07:30.03 Vacuum too high -- waiting up to 40 seconds @19:07:30.04 SS.reconfigure :exhale @19:07:30.09 SS.hold @19:07:51.09 Intake @15.5psia, vacuum from 10.0 to 13.0psi @19:07:51.11 SS.reconfigure :inhaling 0.38ml/s @19:07:51.16 SS.seek 25ml @19:08:09.62 Stroke completed -- waiting for pressure to equalize ... @19:08:09.64 SS.reconfigure :exhale @19:08:09.68 SS.hold @19:08:36.23 Intake.select :close @19:08:36.39 SSV.dial :exhaust @19:08:37.28 Exhaust.select :open @19:08:37.39 SS.seek 0ml @19:08:46.70 Exhaust.select :close @19:08:46.82 SSV.dial CBV @19:08:46.84 CBV.dial SSV @19:08:47.70 Allowing 130s for 25.0ml of remaining 650.0ml @19:08:47.72 Intake.select :open @19:08:49.88 Intake @15.0psia, vacuum from 9.5 to 12.5psi @19:08:49.90 SS.reconfigure :inhaling 0.38ml/s @19:08:49.95 SS.seek 25ml @19:09:54.40 Vacuum too high -- waiting up to 40 seconds @19:09:54.41 SS.reconfigure :exhale @19:09:54.47 SS.hold @19:10:19.58 Intake @15.9psia, vacuum from 10.0 to 13.0psi @19:10:19.60 SS.reconfigure :inhaling 0.31ml/s @19:10:19.65 SS.seek 25ml @19:10:29.37 Stroke completed -- waiting for pressure to equalize ... @19:10:29.38 SS.reconfigure :exhale @19:10:29.43 SS.hold @19:10:56.18 Intake.select :close @19:10:56.34 SSV.dial :exhaust @19:10:57.20 Exhaust.select :open @19:10:57.32 SS.seek 0ml @19:11:06.64 Exhaust.select :close @19:11:06.75 SSV.dial CBV @19:11:06.77 CBV.dial SSV @19:11:07.64 Allowing 130s for 25.0ml of remaining 625.0ml @19:11:07.65 Intake.select :open @19:11:09.82 Intake @14.9psia, vacuum from 9.4 to 12.4psi @19:11:10.29 SS.reconfigure :inhaling 0.31ml/s @19:11:10.35 SS.seek 25ml @19:12:45.52 Stroke completed -- waiting for pressure to equalize ... @19:12:45.54 SS.reconfigure :exhale @19:12:45.59 SS.hold @19:13:17.66 SS.reconfigure :default @19:13:17.72 SS.hold @19:13:17.78 Intake.select :close @19:13:17.94 ShallowSampler::Clogged in WCR -- Filtering too Slowly after 400.0ml @19:13:17.96 SSV.dial :exhaust @19:13:18.82 Exhaust.select :open @19:13:18.94 SS.reconfigure :exhale @19:13:18.99 SS.seek 0ml @19:13:28.40 Exhaust.select :close @19:13:28.51 Sampled 400.0ml @19:13:28.53 Drying puck before fixing for 5:00 @19:13:28.54 CBV.dial :waste2b @19:13:29.39 delay 1:00 @19:13:57.62 recoverPRVreagent08 @19:13:57.63 PTV.dial :mixing @19:13:57.81 PBV.dial :waste1 @19:13:58.22 delay 30 seconds @19:14:28.24 PBV.dial between PSV and waste1 @19:14:28.66 PSV.dial :air @19:14:29.30 PS.reconfigure :fast @19:14:29.35 PS.seek 2.5ml @19:14:29.41 CSV.dial :air @19:14:29.91 CS.reconfigure :fast @19:14:29.96 CS.seek 10ml @19:14:57.97 PSV.dial :mixloop @19:14:58.47 delay 2 seconds @19:14:59.35 Pumping wcr puck from tube 2 up to 35psi... @19:14:59.36 CBV.dial SSV @19:15:00.48 PSV.dial :air @19:15:00.74 CBV.dial between CSV and waste2b @19:15:01.00 delay 2 seconds @19:15:01.82 CSV.dial between air and CTV @19:15:02.18 CS.seek 7.296ml @19:15:03.02 PSV.dial :mixloop @19:15:03.55 delay 2 seconds @19:15:05.56 PSV.dial :air @19:15:06.21 PS.seek 0ml @19:15:10.41 CTV.dial CSV,avoiding: :air @19:15:10.46 CSV.dial CTV @19:15:11.35 CS.reconfigure :pressurize @19:15:11.40 CS.seek 0ml @19:15:32.25 CSV.dial :air @19:15:33.18 CS.reconfigure :fast @19:15:33.24 CS.seek 10ml @19:15:34.47 washCycleDA #1 of 3 @19:15:34.48 PSR.select :air @19:15:34.49 PSV.dial PSR @19:15:36.06 Pulling 0.5ml of PSR.wash @19:15:36.07 PSR.select :wash @19:15:36.19 PS.reconfigure :default @19:15:36.24 PS.seek 0.5ml @19:15:42.65 delay 5 seconds @19:15:47.68 PSR.select :air @19:15:47.80 PS.seek 2ml @19:16:02.24 CSV.dial between air and CTV @19:16:02.60 CS.seek 4.43ml @19:16:05.00 PTV.dial :puck @19:16:05.86 PSV.dial PTV @19:16:06.73 PBV.dial :waste1 @19:16:07.20 PS.seek 1.5ml @19:16:13.34 delay 3 seconds @19:16:16.36 PBV.dial between PSV and waste1 @19:16:16.77 PS.seek 0ml @19:16:18.79 CTV.dial CSV,avoiding: :air @19:16:18.82 CSV.dial CTV @19:16:19.21 CS.reconfigure :pressurize @19:16:19.26 CS.seek 0ml @19:16:32.22 CSV.dial :air @19:16:32.74 CS.reconfigure :fast @19:16:32.79 CS.seek 10ml @19:16:33.97 delay 2:00 @19:17:01.99 CSV.dial between air and CTV @19:17:02.35 CS.seek 4.547ml @19:17:17.97 CTV.dial CSV,avoiding: :air @19:17:18.00 CSV.dial CTV @19:17:18.38 CS.reconfigure :pressurize @19:17:18.43 CS.seek 0ml @19:17:31.71 CSV.dial :air @19:17:32.24 CS.reconfigure :fast @19:17:32.29 CS.seek 10ml @19:18:01.49 CSV.dial between air and CTV @19:18:02.30 CS.seek 4.543ml @19:18:17.84 CTV.dial CSV,avoiding: :air @19:18:17.87 CSV.dial CTV @19:18:18.25 CS.reconfigure :pressurize @19:18:18.30 CS.seek 0ml @19:18:31.65 CSV.dial :air @19:18:32.17 CS.reconfigure :default @19:18:32.22 CS.hold @19:18:32.35 ShallowSampler::Error in MAIN -- Puck.from 2, :wcr reached only 19.9 of 35psi after pumping in 40ml of gas @19:18:32.37 Waiting to Resume from Checkpoint "Pumping wcr puck from tube 2 up to 35psi..." @19:18:32.39 BadNews.email "Puck.from 2, :wcr reached only 19.9 of 35psi after pumping in 40ml of gas",Subject: "ShallowSampler::Error in MAIN" @19:18:33.99 recoverPSRreagent08 @19:18:34.01 PTV.dial :puck @19:18:34.09 PBV.dial :waste1 @19:18:34.50 delay 30 seconds @19:18:36.55 upsync @19:19:04.52 PBV.dial between PSV and waste1 @19:19:04.93 PSR.select :air @19:19:04.95 PSV.dial PSR @19:19:05.83 PS.reconfigure :fast @19:19:05.88 PS.seek 2.5ml @19:19:34.49 PSV.dial PTV @19:19:35.39 delay 5 seconds @19:19:40.41 PSV.dial PSR @19:19:41.29 delay 5 seconds @19:19:46.31 PSV.dial PTV @19:19:47.18 delay 5 seconds @19:19:52.20 PSV.dial :air @19:19:53.08 PS.seek 0ml @19:20:21.15 washCycleDA #2 of 3 @19:20:21.16 PSR.select :air @19:20:21.18 PSV.dial PSR @19:20:22.74 Pulling 0.5ml of PSR.wash @19:20:22.75 PSR.select :wash @19:20:22.87 PS.reconfigure :default @19:20:22.92 PS.seek 0.5ml @19:20:29.30 delay 5 seconds @19:20:34.32 PSR.select :air @19:20:34.45 PS.seek 2ml @19:20:51.71 PTV.dial :puck @19:20:51.79 PSV.dial PTV @19:20:52.65 PBV.dial :waste1 @19:20:53.12 PS.seek 1.5ml @19:20:59.37 delay 3 seconds @19:21:02.39 PBV.dial between PSV and waste1 @19:21:02.79 PS.seek 0ml @19:21:20.55 delay 2:00 @19:23:20.58 recoverPSRreagent08 @19:23:20.59 PTV.dial :puck @19:23:20.66 PBV.dial :waste1 @19:23:21.07 delay 30 seconds @19:23:51.09 PBV.dial between PSV and waste1 @19:23:51.50 PSR.select :air @19:23:51.52 PSV.dial PSR @19:23:52.40 PS.reconfigure :fast @19:23:52.45 PS.seek 2.5ml @19:24:21.36 PSV.dial PTV @19:24:22.24 delay 5 seconds @19:24:27.26 PSV.dial PSR @19:24:28.13 delay 5 seconds @19:24:33.15 PSV.dial PTV @19:24:34.02 delay 5 seconds @19:24:39.04 PSV.dial :air @19:24:39.91 PS.seek 0ml @19:25:07.86 washCycleDA #3 of 3 @19:25:07.87 PSR.select :air @19:25:07.89 PSV.dial PSR @19:25:09.45 Pulling 0.5ml of PSR.wash @19:25:09.46 PSR.select :wash @19:25:09.58 PS.reconfigure :default @19:25:09.63 PS.seek 0.5ml @19:25:15.99 delay 5 seconds @19:25:21.01 PSR.select :air @19:25:21.13 PS.seek 2ml @19:25:38.58 PTV.dial :puck @19:25:38.66 PSV.dial PTV @19:25:39.52 PBV.dial :waste1 @19:25:39.98 PS.seek 1.5ml @19:25:46.14 delay 3 seconds @19:25:49.16 PBV.dial between PSV and waste1 @19:25:49.57 PS.seek 0ml @19:26:07.08 delay 2:00 @19:28:07.11 recoverPSRreagent08 @19:28:07.12 PTV.dial :puck @19:28:07.18 PBV.dial :waste1 @19:28:07.61 delay 30 seconds @19:28:37.63 PBV.dial between PSV and waste1 @19:28:38.04 PSR.select :air @19:28:38.06 PSV.dial PSR @19:28:38.95 PS.reconfigure :fast @19:28:39.00 PS.seek 2.5ml @19:29:07.76 PSV.dial PTV @19:29:08.64 delay 5 seconds @19:29:13.66 PSV.dial PSR @19:29:14.55 delay 5 seconds @19:29:19.57 PSV.dial PTV @19:29:20.43 delay 5 seconds @19:29:25.46 PSV.dial :air @19:29:26.33 PS.seek 0ml @19:29:54.35 Gate.power :camera,:ON @19:29:54.37 Mixing DA Substrates @19:29:54.38 PSR.select :air @19:29:54.39 PSV.dial PSR @19:29:55.98 Pulling 0.25ml of PSR.sub1 @19:29:56.01 PSR.select :sub1 @19:29:56.23 PS.reconfigure :default @19:29:56.34 PS.seek 0.25ml @19:30:00.17 delay 5 seconds @19:30:05.19 Pulling 0.25ml of PSR.sub2 @19:30:05.21 PSR.select :sub2 @19:30:05.32 PS.seek 0.5ml @19:30:08.79 delay 5 seconds @19:30:13.81 PSR.select :air @19:30:14.40 PS.seek 2ml @19:30:31.85 PTV.dial :puck @19:30:31.93 PSV.dial PTV @19:30:32.79 PBV.dial :waste1 @19:30:33.26 PS.seek 1.5ml @19:30:39.51 delay 3 seconds @19:30:42.53 PBV.dial between PSV and waste1 @19:30:42.94 PS.seek 0ml @19:31:00.45 delay 10 seconds @19:31:10.48 recoverPSRreagent08 @19:31:10.49 PTV.dial :puck @19:31:10.55 PBV.dial :waste1 @19:31:10.96 delay 30 seconds @19:31:40.99 PBV.dial between PSV and waste1 @19:31:41.40 PSR.select :air @19:31:41.42 PSV.dial PSR @19:31:42.29 PS.reconfigure :fast @19:31:42.35 PS.seek 2.5ml @19:32:10.94 PSV.dial PTV @19:32:11.84 delay 5 seconds @19:32:16.86 PSV.dial PSR @19:32:17.71 delay 5 seconds @19:32:22.73 PSV.dial PTV @19:32:23.60 delay 5 seconds @19:32:28.62 PSV.dial :air @19:32:29.51 PS.seek 0ml @19:32:58.04 Additional air clear @19:32:58.05 PSR.select :air @19:32:58.07 PSV.dial PSR @19:32:59.62 Pulling 2ml of PSR.air @19:32:59.63 PSR.select :air @19:32:59.65 PS.reconfigure :default @19:32:59.69 PS.seek 2ml @19:33:22.76 delay 5 seconds @19:33:27.78 PTV.dial :puck @19:33:27.85 PSV.dial PTV @19:33:28.83 PBV.dial :waste1 @19:33:29.30 PS.seek 1.5ml @19:33:35.47 delay 3 seconds @19:33:38.49 PBV.dial between PSV and waste1 @19:33:38.90 delay 1 second @19:33:39.92 PSV.dial :waste1 @19:33:41.14 PS.seek 0ml @19:33:58.56 Processing Puck Heater at 28C before imaging @19:33:58.57 PH.coast @19:33:58.68 Imaging da puck in Processing Clamp while storing it to tube 1 @19:33:58.86 SC.reconfigure :tight @19:33:58.91 SC.seek 1 @19:34:02.54 SC.configure :limp @19:34:02.75 Elbow.reconfigure :tight @19:34:02.80 Elbow.seek 1 @19:34:04.51 Forearm.reconfigure :tight @19:34:05.05 Forearm.seek 1 + 450 counts @19:34:06.17 SE.reconfigure :pick @19:34:06.23 SE.seek :beamBreak @19:34:09.45 SE.configure :default @19:34:09.60 Elbow.reconfigure :limp @19:34:09.66 Forearm.reconfigure :loose @19:34:09.70 Forearm.seek 1 @19:34:10.03 Elbow.hold @19:34:10.10 Elbow.reconfigure :tight @19:34:10.20 SE.seek 5.14 Pucks below Top Plate @19:34:10.74 Forearm.force -20 @19:34:10.79 Forearm.seek 1 + 291 counts @19:34:11.09 Forearm.reconfigure :tight @19:34:11.14 Forearm.seek 1 + 450 counts @19:34:11.32 SE.seek 6.14 Pucks below Top Plate @19:34:11.84 Forearm.reconfigure :loose @19:34:11.89 Forearm.seek :retract @19:34:12.82 Elbow.seek PC @19:34:15.52 Elbow.reconfigure :loose @19:34:15.62 Forearm.seek PC @19:34:16.61 Hand.close @19:34:17.36 PC.seek :open @19:34:22.18 Forearm.seek :retract @19:34:23.16 Elbow.reconfigure :tight @19:34:23.21 Elbow.seek 1 @19:34:25.85 Forearm.reconfigure :tight @19:34:25.90 Forearm.seek 1 + 450 counts @19:34:26.91 Hand.open @19:34:27.67 SE.seek 5.79 Pucks below Top Plate @19:34:27.97 SE.reconfigure :seal @19:34:28.02 SE.seek 4.83 Pucks below Top Plate @19:34:28.59 Lights, camera, ACTION! @19:34:28.63 PuckCamera: snap -bin=2,2 -auto /var/log/esp/da23sep1919h400ml.tif @19:34:48.07 Waiting >20 seconds for da to relinquish FlushPuck @19:34:52.43 SX HX9/16 (USB:02): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 20.312 seconds /var/log/esp/da23sep1919h400ml.tif: TIFF Upload Complete @19:34:52.46 PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da23sep1919h400ml15s.tif @19:35:08.07 SX HX9/16 (USB:02): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15 seconds /var/log/esp/midres/da23sep1919h400ml15s.tif: TIFF Upload Complete @19:35:08.10 PuckCamera: snap -bin=2,2 20.0 /var/log/esp/midres/da23sep1919h400ml20s.tif @19:35:28.75 SX HX9/16 (USB:02): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 20 seconds /var/log/esp/midres/da23sep1919h400ml20s.tif: TIFF Upload Complete @19:35:28.77 Gate.power :camera,:OFF @19:35:28.79 DAfans.email "20.312 sec auto-exposure",Subject: "da23sep1919h400ml images!" @19:35:29.34 Elbow.seek 1 @19:35:29.57 Forearm.seek 1 + 450 counts @19:35:29.64 SE.reconfigure :gently @19:35:29.71 SE.seek 5.54 Pucks below Top Plate @19:35:30.38 Elbow.reconfigure :limp @19:35:30.45 Forearm.reconfigure :loose @19:35:30.50 Forearm.seek 1 @19:35:30.78 Elbow.hold @19:35:30.86 Elbow.reconfigure :tight @19:35:30.92 SE.reconfigure :default @19:35:30.97 SE.seek 5.73 Pucks below Top Plate @19:35:31.39 Forearm.force -20 @19:35:31.44 Forearm.seek 1 + 292 counts @19:35:31.75 Forearm.reconfigure :tight @19:35:31.92 Forearm.seek 1 + 450 counts @19:35:32.25 SE.seek :down @19:35:33.50 Forearm.reconfigure :loose @19:35:33.62 Forearm.seek :retract @19:35:34.61 SE.stop @19:35:34.91 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @19:35:35.31 Elbow.seek FlushPuck::Garage @19:35:37.26 Elbow.reconfigure :loose @19:35:37.48 Forearm.seek :garage @19:35:38.52 Hand.close @19:35:40.57 Forearm.seek :retract @19:35:41.60 Elbow.reconfigure :tight @19:35:41.70 Elbow.seek PC @19:35:43.45 Elbow.reconfigure :loose @19:35:43.69 Forearm.reconfigure :tight @19:35:43.77 Forearm.seek PC @19:35:45.29 PC.seek :closed @19:35:50.21 Processing Clamp onSpring/offSpring for 16/26 samples, on/off torque 2.47 @19:35:50.38 Hand.open @19:35:51.24 Forearm.reconfigure :loose @19:35:51.34 Forearm.seek :retract @19:35:52.39 PSR.select :air @19:35:52.45 PSV.dial PSR @19:35:53.04 Pulling 0.5ml of PSR.flush @19:35:53.07 PSR.select :flush @19:35:53.24 PS.seek 0.5ml @19:35:59.65 delay 5 seconds @19:36:01.82 upsync @19:36:04.67 PSR.select :air @19:36:04.80 PS.seek 2.5ml @19:36:27.88 delay 1 second @19:36:28.90 PRV.dial :waste2,avoiding: :ab2 @19:36:31.10 PTV.dial :PRVmixing @19:36:32.63 PSV.dial :mixloop @19:36:33.83 PS.seek :empty @19:37:01.72 PSR.select :air @19:37:01.74 PSV.dial PSR @19:37:02.95 Pulling 0.5ml of PSR.flush @19:37:02.97 PSR.select :flush @19:37:03.09 PS.seek 0.5ml @19:37:09.44 delay 5 seconds @19:37:14.46 PSR.select :air @19:37:14.58 PS.seek 2.5ml @19:37:37.25 delay 1 second @19:37:38.27 PBV.dial :waste1 @19:37:38.67 PTV.dial :mixing @19:37:39.52 PSV.dial :mixloop @19:37:40.74 PS.seek :empty @19:38:08.97 PSV.dial PSR @19:38:10.19 PSR.select :flush @19:38:10.31 PS.seek 1ml @19:38:22.12 PSR.select :air @19:38:22.24 PS.seek 2.5ml @19:38:39.47 PSV.dial PTV @19:38:40.33 PTV.dial :puck @19:38:41.16 PBV.dial :waste1 @19:38:41.24 PS.seek 0ml @19:39:09.34 PSV.dial PSR @19:39:10.20 PSR.select :flush @19:39:10.32 PS.seek 0.5ml @19:39:16.72 PSR.select :air @19:39:16.84 PS.seek 2.5ml @19:39:39.59 PSV.dial PTV @19:39:40.47 PBV.dial PSV @19:39:41.10 PS.seek 0ml @19:40:09.00 PSV.dial PBV @19:40:09.52 PS.seek 2.5ml @19:40:37.68 PSV.dial :waste1 @19:40:38.52 PS.seek 0ml @19:41:06.53 PSV.dial PSR @19:41:07.03 PSR.select :air @19:41:07.05 PS.seek 2.5ml @19:41:35.40 PSV.dial PTV @19:41:36.23 PBV.dial PSV @19:41:36.31 PS.seek 0ml @19:42:04.34 PSV.dial PBV @19:42:04.86 PS.seek 2.5ml @19:42:33.06 PSV.dial :waste1 @19:42:33.91 PS.seek 0ml @19:43:02.06 PSV.dial PSR @19:43:02.57 PSR.select :flush @19:43:02.69 PS.seek 1ml @19:43:14.55 PSR.select :air @19:43:14.67 PS.seek 2.5ml @19:43:31.99 PSV.dial PTV @19:43:32.95 PTV.dial :puck @19:43:33.03 PBV.dial :waste1 @19:43:33.66 PS.seek 0ml @19:44:01.62 PSV.dial PSR @19:44:02.44 PSR.select :flush @19:44:02.57 PS.seek 0.5ml @19:44:09.06 PSR.select :air @19:44:09.18 PS.seek 2.5ml @19:44:31.87 PSV.dial PTV @19:44:32.73 PBV.dial PSV @19:44:33.36 PS.seek 0ml @19:45:01.46 PSV.dial PBV @19:45:01.98 PS.seek 2.5ml @19:45:30.21 PSV.dial :waste1 @19:45:31.06 PS.seek 0ml @19:45:59.15 PSV.dial PSR @19:45:59.66 PSR.select :air @19:45:59.69 PS.seek 2.5ml @19:46:27.83 PSV.dial PTV @19:46:28.66 PBV.dial PSV @19:46:28.85 PS.seek 0ml @19:46:57.14 PSV.dial PBV @19:46:57.79 PS.seek 2.5ml @19:47:25.99 PSV.dial :waste1 @19:47:26.85 PS.seek 0ml @19:47:54.74 Garaging FlushPuck in Processing Clamp @19:47:54.81 Elbow.reconfigure :tight @19:47:54.86 Elbow.seek PC @19:47:54.98 Elbow.reconfigure :loose @19:47:55.08 Forearm.seek PC @19:47:56.07 Hand.close @19:47:56.85 PC.seek :open @19:48:01.60 Forearm.seek :retract @19:48:02.62 Elbow.reconfigure :tight @19:48:02.67 Elbow.seek :garage @19:48:03.97 Elbow.reconfigure :loose @19:48:04.07 Forearm.reconfigure :tight @19:48:04.12 Forearm.seek :garage @19:48:05.18 Hand.open @19:48:06.03 Forearm.reconfigure :loose @19:48:06.08 Forearm.seek :retract @01:37:41.29UTC20-Sep-23 Session Begun @01:37:48.86 -> CC Collection Clamp contains wcr puck from tube 2 @01:37:51.46 -> PC Processing Clamp open @01:37:58.43 -> Hand Hand is opened @01:38:01.34 -> Arm Elbow at garage, Forearm retracted, Hand is opened @01:39:49.71 -> CC.unloadPuck 1, :WCR @01:39:49.79 Puck::Warning in dave -- Unloading Puck.from 2, :wcr to Storage Carousel @01:39:49.81 Storing WCR puck in Collection Clamp to tube 1 @01:39:50.24 Elbow.reconfigure :tight @01:39:50.31 Elbow.seek 1 @01:39:52.22 Forearm.reconfigure :tight @01:39:52.29 Forearm.seek 1 + 450 counts @01:39:53.44 SE.reconfigure :pick @01:39:53.52 SE.seek :beamBreak @01:39:56.59 SE.configure :default @01:39:56.76 Elbow.reconfigure :limp @01:39:56.83 Forearm.reconfigure :loose @01:39:56.90 Forearm.seek 1 @01:39:57.18 Elbow.hold @01:39:57.26 Elbow.reconfigure :tight @01:39:57.38 SE.seek 6.1 Pucks below Top Plate @01:39:57.87 Forearm.force -20 @01:39:57.93 Forearm.seek 1 + 299 counts @01:39:58.23 Forearm.reconfigure :tight @01:39:58.30 Forearm.seek 1 + 450 counts @01:39:58.53 SE.seek 7.1 Pucks below Top Plate @01:39:59.14 Forearm.reconfigure :loose @01:39:59.21 Forearm.seek :retract @01:40:00.17 Elbow.seek CC @01:40:01.87 Elbow.reconfigure :loose @01:40:01.98 Forearm.seek CC @01:40:03.04 Hand.close @01:40:03.81 CC.seek :open @01:40:08.58 Forearm.seek :retract @01:40:09.59 Elbow.reconfigure :tight @01:40:09.66 Elbow.seek 1 @01:40:11.98 Elbow.reconfigure :limp @01:40:12.05 Forearm.seek 1 @01:40:13.09 Elbow.hold @01:40:13.17 Elbow.reconfigure :tight @01:40:13.24 Hand.open @01:40:14.05 Forearm.force -20 @01:40:14.11 Forearm.seek 1 + 302 counts @01:40:14.42 Forearm.reconfigure :tight @01:40:14.49 Forearm.seek 1 + 450 counts @01:40:14.77 SE.seek :down @01:40:15.91 Forearm.reconfigure :loose @01:40:15.98 Forearm.seek :retract @01:40:17.10 SE.stop @01:41:00.12 -> CC Collection Clamp open @01:41:04.33 -> PC Processing Clamp open @01:51:10.95 -> ps aux @01:51:10.98 NoMethodError in dave -- undefined method `ps' for CmdServer::Client:Class @01:51:19.00 -> exit @01:51:19.01 Session Ended + ESPwhich DA_WCR_92623.rb + fn=DA_WCR_92623.rb + IFS=: + set /home/esp/esp2/mission . + fullPath=/home/esp/esp2/mission/DA_WCR_92623.rb + [ -f /home/esp/esp2/mission/DA_WCR_92623.rb ] + realpath /home/esp/esp2/mission/DA_WCR_92623.rb + return 0 + scriptPath=/home/esp/esp2local/mission/DA_WCR_92623.rb + exec ruby /home/esp/esp2/lib/esp.rb /home/esp/esp2local/mission/DA_WCR_92623.rb @17:42:01.79UTC26-Sep-23
ESP2 v3.42 I2C Gateway v3.20 for core bus @17:42:04.44 Configuration for short puck jake 1/11/21 by brent@mbari.org /home/esp/esp2/type/shallow/jake/configure.rb @17:42:08.09 Polling Can every 10:00 @17:42:08.14 Cleanly exited at 17:41:41UTC26-Sep-23 @17:42:08.18 Loading /home/esp/esp2local/mission/DA_WCR_92623.rb mission @17:42:08.24 Preparing to sleep until + 00:00:05 @17:42:08.27 GoodNews.email "Starting DA_WCR_92623 w/pucks in tube 2\n{1 => 8,\n 2 => 25,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@17:42:08, 26% flow, 18.3C, 11% humidity, 14.6psia, 13.754V, 0.260A, 45.513Ah, 3.58W",Subject: "DA_WCR_92623 begun" @17:42:08.32
Asleep til + 00:00:05 @17:42:08.34 Continuing at 17:42 @17:42:08.58 Gate.power :core,:OFF @17:42:08.59 Gate.power :raw,:OFF @17:42:08.60 Shutdown @17:42:10.90 GoodNews.email "Then, in tube 2: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_92623.rb:14\n{1 => 8,\n 2 => 25,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@17:42:08, 26% flow, 18.3C, 11% humidity, 14.6psia, 13.754V, 0.254A, 45.513Ah, 3.50W",Subject: "Asleep until 17:42" @17:42:12.30 upsync @17:42:13.23
Powering core bus @17:42:14.79 upsync @17:42:18.93
Gate.power :raw,:ON @17:42:19.98 CS.configure :default @17:42:20.03 PS.configure :default @17:42:20.08 SS.configure :default @17:42:20.13 CC.configure :default @17:42:20.18 PC.configure :default @17:42:20.24 Forearm.configure :loose @17:42:20.29 Elbow.configure :loose @17:42:20.36 SC.configure :loose @17:42:20.41 SE.configure :default @17:42:20.46 CH.configure :default @17:42:20.49 PH.configure :default @17:42:20.53 All dwarf controllers running firmware version 3.85 @17:42:20.64 Valve.reset @17:42:20.95 Intake.select :close @17:42:20.96 Exhaust.select :close @17:42:20.97 CTV.dial :air @17:42:21.04 PSV.dial :air @17:42:21.17
Forearm.home.seek :retract @17:42:21.92 Hand.close @17:42:21.96
Elbow.reconfigure :loose @17:42:22.04 Elbow.jog 1500 @17:42:22.28 PS.home.seek 0ml @17:42:22.35 CSV.dial :waste1 @17:42:22.46
Elbow.reconfigure :loose @17:42:22.51 Elbow.home.seek :clear @17:42:23.07 SSV.dial :spit,avoiding: :exhaust @17:42:23.96 CS.reconfigure :fast @17:42:24.01 SS.home.seek 0ml @17:42:24.10 CS.home.seek 0ml @17:42:26.96
SE.home.seek :down @17:42:27.56 SC.reconfigure :tight @17:42:27.61 SC.home.seek :initial @17:42:30.63 PS.stop @17:42:55.95
SC.configure :limp @17:42:56.01 Forearm.seek :retract @17:42:56.07 Elbow.seek :hammer @17:42:57.09 Forearm.reconfigure :tight @17:42:57.14 Forearm.seek retracted @17:42:57.44 CC.home.seek :open @17:42:57.76 PC.home.seek :open @17:43:01.26
Phase [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] @17:43:01.27 Notice.email "At 17:43:01 began in tube 2: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_92623.rb:14\n{1 => 8,\n 2 => 25,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@17:43:01, 26% flow, 18.2C, 11% humidity, 14.6psia, 13.739V, 0.248A, 45.518Ah, 3.41W",Subject: "Started DA_WCR_92623 phase initialPurge; da [1000, 1000] #da and wsr are run simultanelously" @17:43:01.30
Starting initialPurge @17:43:01.31 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @17:43:01.38 Hand.open @17:43:02.18 Elbow.reconfigure :tight @17:43:02.23 Elbow.seek FlushPuck::Garage @17:43:04.20 Elbow.reconfigure :loose @17:43:04.41 Forearm.reconfigure :loose @17:43:04.48 Forearm.seek :garage @17:43:05.43 Hand.close @17:43:05.51 upsync @17:43:06.17
Forearm.seek :retract @17:43:07.12 Elbow.reconfigure :tight @17:43:07.17 Elbow.seek CC @17:43:10.08 Elbow.reconfigure :loose @17:43:10.19 Forearm.reconfigure :tight @17:43:10.24 Forearm.seek CC @17:43:11.51 CC.seek :closed @17:43:16.09 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.41 @17:43:16.16 Hand.open @17:43:16.85 Forearm.reconfigure :loose @17:43:16.90 Forearm.seek :retract @17:43:17.95 Flushing the Collection Valve::Manifold @17:43:18.07 CSV.dial CSR @17:43:19.35 Pulling 4.999ml of CSR.flush @17:43:19.36 CSR.select :flush @17:43:19.48 CS.reconfigure :default @17:43:19.93 CS.seek 5ml @17:43:33.52 delay 5 seconds @17:43:38.54 CSR.select :air @17:43:38.71 CS.reconfigure :fast @17:43:38.76 CS.seek 7.5ml @17:43:45.49 CSV.dial :waste @17:43:46.78 CS.reconfigure :default @17:43:46.82 CS.seek 0ml @17:44:06.83 Purging external Intake @17:44:06.96 CSV.dial :flush @17:44:07.42 CS.reconfigure :fast @17:44:07.47 CS.seek 10ml @17:44:31.90 delay 5 seconds @17:44:36.92 CSV.dial CBV @17:44:36.95
CBV.dial CSV @17:44:37.74 CTV.dial :intake @17:44:38.50 Intake.select :open @17:44:38.62 CS.seek 0ml @17:45:01.92 Intake.select :close @17:45:02.03 CTV.dial :air @17:45:02.82 Flushing upper manifold @17:45:02.94 CSV.dial :flush @17:45:03.68 CS.seek 5ml @17:45:16.39 delay 5 seconds @17:45:21.41 CSV.dial :air @17:45:21.91 CS.seek 7ml @17:45:27.49 CSV.dial CBV @17:45:27.53
CBV.dial CSV @17:45:28.53 CTV.dial :air @17:45:28.62 CS.seek 0ml @17:45:45.08 delay 7 seconds @17:45:52.11 CS.seek 3ml @17:46:00.02 CSV.dial :waste @17:46:00.48 CS.seek 0ml @17:46:08.16 CSV.dial :flush @17:46:08.60 CS.seek 3ml @17:46:16.55 delay 5 seconds @17:46:21.57 CSV.dial :air @17:46:22.07 CS.seek 5ml @17:46:27.58 CSV.dial :air @17:46:27.66 CS.seek 0ml @17:46:39.80 delay 7 seconds @17:46:46.83 CS.seek 3ml @17:46:54.95 CSV.dial :waste @17:46:55.70 CS.seek 0ml @17:47:03.33 Garaging FlushPuck in Collection Clamp @17:47:03.40 Elbow.reconfigure :tight @17:47:03.45 Elbow.seek CC @17:47:03.79 Elbow.reconfigure :loose @17:47:03.90 Forearm.seek CC @17:47:04.93 Hand.close @17:47:05.54 CC.seek :open @17:47:10.61 Forearm.seek :retract @17:47:11.60 Elbow.reconfigure :tight @17:47:11.65 Elbow.seek :garage @17:47:14.53 Elbow.reconfigure :loose @17:47:14.63 Forearm.reconfigure :tight @17:47:14.68 Forearm.seek :garage @17:47:15.65 Hand.open @17:47:16.35 Forearm.reconfigure :loose @17:47:16.40 Forearm.seek :retract @17:47:17.37 da sampling at most 1000ml, wcr at most 1000ml @17:47:17.41 Loading sh1 puck from tube 2 into Collection Clamp @17:47:17.42 DAfans.email "Started da in tube 2 at 17:47:17UTC26-Sep-23\n{1 => 8,\n 2 => 25,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@17:43:01, 26% flow, 18.2C, 11% humidity, 14.6psia, 13.739V, 0.248A, 45.518Ah, 3.41W",Subject: "da sampling at most 1000ml, wcr at most 1000ml" @17:47:17.70
SC.reconfigure :tight @17:47:17.77 SC.seek 2 @17:47:21.26 upsync @17:47:24.53
SC.configure :limp @17:47:24.76 Elbow.reconfigure :tight @17:47:24.81 Elbow.seek 2 @17:47:26.75 Forearm.reconfigure :tight @17:47:26.80 Forearm.seek 2 + 450 counts @17:47:27.90 SE.reconfigure :pick @17:47:27.96 SE.seek :beamBreak @17:47:29.57 SE.configure :default @17:47:29.74 Elbow.seek 2 @17:47:29.93 Forearm.seek 2 + 300 counts @17:47:30.10 Forearm.reconfigure :loose @17:47:30.15 Hand.close @17:47:30.80 SE.seek :down @17:47:31.38
Forearm.seek :retract @17:47:31.96 SE.stop @17:47:32.38
Elbow.seek CC @17:47:34.02 Elbow.reconfigure :loose @17:47:34.12 Forearm.reconfigure :tight @17:47:34.17 Forearm.seek CC @17:47:35.47 CC.seek :closed @17:47:40.00 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.26 @17:47:40.01 Hand.open @17:47:40.72 Forearm.reconfigure :loose @17:47:40.77 Forearm.seek :retract @17:47:41.77 collectSample 1000ml w/bubble point of 24psi @17:47:41.78 Priming 7ml @17:47:41.79 Intake.select :close @17:47:41.81 Exhaust.select :close @17:47:41.82 SS.hold @17:47:42.04 Calibrating pressure and temperature sensors @17:47:42.06 CTV.dial :air @17:47:42.08 SSV.dial :spit,avoiding: :exhaust @17:47:42.27 SS.reconfigure :fast @17:47:42.32 SS.seek 5ml @17:47:45.03 SSV.dial CBV @17:47:45.07
CBV.dial SSV @17:47:46.41 delay 3.8 seconds @17:47:50.33 Sensor limits: Intake max 117.3psia; Outlet max 110.5psia @17:47:50.34 SSV.dial :spit @17:47:51.66 SS.seek 0ml @17:47:54.40 SSV.dial CBV @17:47:55.70 CTV.dial :intake @17:47:56.50 SSV.dial CBV @17:47:56.53
CBV.dial SSV @17:47:56.61 Allowing 40s for 7.0ml of remaining 7.0ml @17:47:56.62 Intake.select :open @17:47:58.78 Intake @14.8psia, vacuum from 9.3 to 12.3psi @17:47:58.80 SS.reconfigure :inhaling 2.20ml/s @17:47:58.85 SS.seek 7ml @17:48:05.70 Stroke completed -- waiting for pressure to equalize ... @17:48:05.71 SS.reconfigure :exhale @17:48:05.75 SS.hold @17:48:11.61 Intake.select :close @17:48:11.76 SSV.dial :exhaust @17:48:12.51 Exhaust.select :open @17:48:12.62 SS.seek 0ml @17:48:16.74 Exhaust.select :close @17:48:16.86 Sampling 1000.0ml @17:48:16.87 Intake.select :close @17:48:16.88 Exhaust.select :close @17:48:16.89 SS.reconfigure :default @17:48:16.93 SS.hold @17:48:17.14 CTV.dial :intake @17:48:17.22 SSV.dial CBV @17:48:17.25
CBV.dial SSV @17:48:18.00 Allowing 130s for 25.0ml of remaining 1000.0ml @17:48:18.01 Intake.select :open @17:48:20.17 Intake @15.3psia, vacuum from 9.8 to 12.8psi @17:48:20.19 SS.reconfigure :inhaling 2.20ml/s @17:48:20.24 SS.seek 25ml @17:48:28.96 Vacuum too high -- waiting up to 40 seconds @17:48:28.97 SS.reconfigure :exhale @17:48:29.02 SS.hold @17:48:31.44 Intake @12.9psia, vacuum from 7.4 to 10.4psi @17:48:31.46 SS.reconfigure :inhaling 1.76ml/s @17:48:31.50 SS.seek 25ml @17:48:38.67 Vacuum too high -- waiting up to 40 seconds @17:48:38.69 SS.reconfigure :exhale @17:48:38.74 SS.hold @17:48:43.79 Intake @15.1psia, vacuum from 9.6 to 12.6psi @17:48:43.81 SS.reconfigure :inhaling 1.41ml/s @17:48:43.86 SS.seek 25ml @17:48:44.29 Stroke completed -- waiting for pressure to equalize ... @17:48:44.30 SS.reconfigure :exhale @17:48:44.35 SS.hold @17:48:46.36 Intake.select :close @17:48:46.51 SSV.dial :exhaust @17:48:47.26 Exhaust.select :open @17:48:47.37 SS.seek 0ml @17:48:55.90 Exhaust.select :close @17:48:56.01 SSV.dial CBV @17:48:56.03
CBV.dial SSV @17:48:56.79 Allowing 130s for 25.0ml of remaining 975.0ml @17:48:56.80 Intake.select :open @17:48:58.97 Intake @15.7psia, vacuum from 10.0 to 13.0psi @17:48:58.99 SS.reconfigure :inhaling 1.41ml/s @17:48:59.03 SS.seek 25ml @17:49:18.86 Vacuum too high -- waiting up to 40 seconds @17:49:18.87 SS.reconfigure :exhale @17:49:18.93 SS.hold @17:49:22.10 Intake @14.4psia, vacuum from 8.9 to 11.9psi @17:49:22.12 SS.reconfigure :inhaling 1.13ml/s @17:49:22.16 SS.seek 25ml @17:49:23.79 Stroke completed -- waiting for pressure to equalize ... @17:49:23.80 SS.reconfigure :exhale @17:49:23.84 SS.hold @17:49:29.76 Intake.select :close @17:49:29.91 SSV.dial :exhaust @17:49:30.67 Exhaust.select :open @17:49:30.78 SS.seek 0ml @17:49:39.71 Exhaust.select :close @17:49:39.82 SSV.dial CBV @17:49:39.85
CBV.dial SSV @17:49:40.59 Allowing 130s for 25.0ml of remaining 950.0ml @17:49:40.60 Intake.select :open @17:49:42.76 Intake @15.6psia, vacuum from 10.0 to 13.0psi @17:49:42.78 SS.reconfigure :inhaling 1.13ml/s @17:49:42.83 SS.seek 25ml @17:50:07.26 Stroke completed -- waiting for pressure to equalize ... @17:50:07.27 SS.reconfigure :exhale @17:50:07.31 SS.hold @17:50:13.86 Intake.select :close @17:50:14.01 SSV.dial :exhaust @17:50:14.76 Exhaust.select :open @17:50:14.87 SS.seek 0ml @17:50:23.88 Exhaust.select :close @17:50:23.99 SSV.dial CBV @17:50:24.03
CBV.dial SSV @17:50:24.76 Allowing 130s for 25.0ml of remaining 925.0ml @17:50:24.77 Intake.select :open @17:50:26.94 Intake @15.6psia, vacuum from 10.0 to 13.0psi @17:50:26.96 SS.reconfigure :inhaling 1.13ml/s @17:50:27.01 SS.seek 25ml @17:50:51.82 Stroke completed -- waiting for pressure to equalize ... @17:50:51.83 SS.reconfigure :exhale @17:50:51.88 SS.hold @17:51:00.43 Intake.select :close @17:51:00.58 SSV.dial :exhaust @17:51:01.34 Exhaust.select :open @17:51:01.45 SS.seek 0ml @17:51:10.21 Exhaust.select :close @17:51:10.32 SSV.dial CBV @17:51:10.36
CBV.dial SSV @17:51:11.09 Allowing 130s for 25.0ml of remaining 900.0ml @17:51:11.10 Intake.select :open @17:51:13.26 Intake @15.6psia, vacuum from 10.0 to 13.0psi @17:51:13.28 SS.reconfigure :inhaling 1.13ml/s @17:51:13.33 SS.seek 25ml @17:51:38.05 Stroke completed -- waiting for pressure to equalize ... @17:51:38.06 SS.reconfigure :exhale @17:51:38.11 SS.hold @17:51:47.21 Intake.select :close @17:51:47.36 SSV.dial :exhaust @17:51:48.12 Exhaust.select :open @17:51:48.23 SS.seek 0ml @17:51:56.97 Exhaust.select :close @17:51:57.09 SSV.dial CBV @17:51:57.11
CBV.dial SSV @17:51:57.87 Allowing 130s for 25.0ml of remaining 875.0ml @17:51:57.88 Intake.select :open @17:52:00.04 Intake @15.5psia, vacuum from 10.0 to 13.0psi @17:52:00.06 SS.reconfigure :inhaling 1.13ml/s @17:52:00.11 SS.seek 25ml @17:52:24.93 Stroke completed -- waiting for pressure to equalize ... @17:52:24.94 SS.reconfigure :exhale @17:52:24.98 SS.hold @17:52:36.11 Intake.select :close @17:52:36.70 SSV.dial :exhaust @17:52:37.46 Exhaust.select :open @17:52:37.57 SS.seek 0ml @17:52:46.57 Exhaust.select :close @17:52:46.68 SSV.dial CBV @17:52:46.71
CBV.dial SSV @17:52:47.48 Allowing 130s for 25.0ml of remaining 850.0ml @17:52:47.49 Intake.select :open @17:52:49.65 Intake @15.4psia, vacuum from 9.9 to 12.9psi @17:52:49.67 SS.reconfigure :inhaling 1.13ml/s @17:52:49.72 SS.seek 25ml @17:53:14.19 Stroke completed -- waiting for pressure to equalize ... @17:53:14.20 SS.reconfigure :exhale @17:53:14.25 SS.hold @17:53:26.82 Intake.select :close @17:53:26.97 SSV.dial :exhaust @17:53:27.74 Exhaust.select :open @17:53:27.86 SS.seek 0ml @17:53:36.86 Exhaust.select :close @17:53:36.97 SSV.dial CBV @17:53:37.00
CBV.dial SSV @17:53:37.77 Allowing 130s for 25.0ml of remaining 825.0ml @17:53:37.78 Intake.select :open @17:53:39.94 Intake @15.3psia, vacuum from 9.8 to 12.8psi @17:53:39.96 SS.reconfigure :inhaling 1.13ml/s @17:53:40.01 SS.seek 25ml @17:54:03.23 Vacuum too high -- waiting up to 40 seconds @17:54:03.24 SS.reconfigure :exhale @17:54:03.29 SS.hold @17:54:10.18 Intake @14.6psia, vacuum from 9.1 to 12.1psi @17:54:10.20 SS.reconfigure :inhaling 0.91ml/s @17:54:10.24 SS.seek 25ml @17:54:12.25 Stroke completed -- waiting for pressure to equalize ... @17:54:12.26 SS.reconfigure :exhale @17:54:12.31 SS.hold @17:54:18.84 Intake.select :close @17:54:18.99 SSV.dial :exhaust @17:54:19.75 Exhaust.select :open @17:54:19.87 SS.seek 0ml @17:54:28.86 Exhaust.select :close @17:54:28.97 SSV.dial CBV @17:54:29.00
CBV.dial SSV @17:54:29.75 Allowing 130s for 25.0ml of remaining 800.0ml @17:54:29.77 Intake.select :open @17:54:31.93 Intake @15.2psia, vacuum from 9.7 to 12.7psi @17:54:31.95 SS.reconfigure :inhaling 0.91ml/s @17:54:32.42 SS.seek 25ml @17:55:01.78 Stroke completed -- waiting for pressure to equalize ... @17:55:01.79 SS.reconfigure :exhale @17:55:01.84 SS.hold @17:55:16.21 Intake.select :close @17:55:16.36 SSV.dial :exhaust @17:55:17.11 Exhaust.select :open @17:55:17.22 SS.seek 0ml @17:55:25.89 Exhaust.select :close @17:55:26.00 SSV.dial CBV @17:55:26.02
CBV.dial SSV @17:55:26.77 Allowing 130s for 25.0ml of remaining 775.0ml @17:55:26.78 Intake.select :open @17:55:28.94 Intake @15.0psia, vacuum from 9.5 to 12.5psi @17:55:28.96 SS.reconfigure :inhaling 0.91ml/s @17:55:29.01 SS.seek 25ml @17:55:57.20 Vacuum too high -- waiting up to 40 seconds @17:55:57.21 SS.reconfigure :exhale @17:55:57.26 SS.hold @17:56:06.40 Intake @14.6psia, vacuum from 9.1 to 12.1psi @17:56:06.41 SS.reconfigure :inhaling 0.73ml/s @17:56:06.46 SS.seek 25ml @17:56:09.34 Stroke completed -- waiting for pressure to equalize ... @17:56:09.35 SS.reconfigure :exhale @17:56:09.40 SS.hold @17:56:16.79 Intake.select :close @17:56:16.94 SSV.dial :exhaust @17:56:17.69 Exhaust.select :open @17:56:17.81 SS.seek 0ml @17:56:26.55 Exhaust.select :close @17:56:26.66 SSV.dial CBV @17:56:26.70
CBV.dial SSV @17:56:27.43 Allowing 130s for 25.0ml of remaining 750.0ml @17:56:27.44 Intake.select :open @17:56:29.60 Intake @14.9psia, vacuum from 9.4 to 12.4psi @17:56:29.62 SS.reconfigure :inhaling 0.73ml/s @17:56:29.67 SS.seek 25ml @17:57:06.15 Stroke completed -- waiting for pressure to equalize ... @17:57:06.16 SS.reconfigure :exhale @17:57:06.21 SS.hold @17:57:22.93 Intake.select :close @17:57:23.08 SSV.dial :exhaust @17:57:23.83 Exhaust.select :open @17:57:23.94 SS.seek 0ml @17:57:32.89 Exhaust.select :close @17:57:33.00 SSV.dial CBV @17:57:33.04
CBV.dial SSV @17:57:33.77 Allowing 130s for 25.0ml of remaining 725.0ml @17:57:33.78 Intake.select :open @17:57:35.94 Intake @14.7psia, vacuum from 9.2 to 12.2psi @17:57:35.96 SS.reconfigure :inhaling 0.73ml/s @17:57:36.01 SS.seek 25ml @17:58:10.97 Vacuum too high -- waiting up to 40 seconds @17:58:10.99 SS.reconfigure :exhale @17:58:11.04 SS.hold @17:58:22.40 Intake @14.8psia, vacuum from 9.3 to 12.3psi @17:58:22.42 SS.reconfigure :inhaling 0.59ml/s @17:58:22.46 SS.seek 25ml @17:58:24.23 Stroke completed -- waiting for pressure to equalize ... @17:58:24.24 SS.reconfigure :exhale @17:58:24.29 SS.hold @17:58:32.84 Intake.select :close @17:58:32.99 SSV.dial :exhaust @17:58:33.73 Exhaust.select :open @17:58:33.85 SS.seek 0ml @17:58:42.58 Exhaust.select :close @17:58:42.69 SSV.dial CBV @17:58:42.71
CBV.dial SSV @17:58:43.47 Allowing 130s for 25.0ml of remaining 700.0ml @17:58:43.48 Intake.select :open @17:58:46.07 Intake @14.7psia, vacuum from 9.2 to 12.2psi @17:58:46.09 SS.reconfigure :inhaling 0.59ml/s @17:58:46.14 SS.seek 25ml @17:59:31.21 Stroke completed -- waiting for pressure to equalize ... @17:59:31.22 SS.reconfigure :exhale @17:59:31.27 SS.hold @17:59:51.70 Intake.select :close @17:59:51.86 SSV.dial :exhaust @17:59:52.61 Exhaust.select :open @17:59:52.73 SS.seek 0ml @18:00:01.71 Exhaust.select :close @18:00:01.83 SSV.dial CBV @18:00:01.85
CBV.dial SSV @18:00:02.61 Allowing 130s for 25.0ml of remaining 675.0ml @18:00:02.62 Intake.select :open @18:00:04.78 Intake @14.5psia, vacuum from 9.0 to 12.0psi @18:00:04.80 SS.reconfigure :inhaling 0.59ml/s @18:00:04.85 SS.seek 25ml @18:00:48.72 Vacuum too high -- waiting up to 40 seconds @18:00:48.73 SS.reconfigure :exhale @18:00:48.79 SS.hold @18:01:03.57 Intake @14.9psia, vacuum from 9.4 to 12.4psi @18:01:03.58 SS.reconfigure :inhaling 0.47ml/s @18:01:03.63 SS.seek 25ml @18:01:04.70 Stroke completed -- waiting for pressure to equalize ... @18:01:04.71 SS.reconfigure :exhale @18:01:04.75 SS.hold @18:01:14.54 Intake.select :close @18:01:14.69 SSV.dial :exhaust @18:01:15.44 Exhaust.select :open @18:01:15.55 SS.seek 0ml @18:01:24.28 Exhaust.select :close @18:01:24.39 SSV.dial CBV @18:01:24.42
CBV.dial SSV @18:01:25.17 Allowing 130s for 25.0ml of remaining 650.0ml @18:01:25.18 Intake.select :open @18:01:27.34 Intake @14.5psia, vacuum from 9.0 to 12.0psi @18:01:27.36 SS.reconfigure :inhaling 0.47ml/s @18:01:27.41 SS.seek 25ml @18:02:23.37 Stroke completed -- waiting for pressure to equalize ... @18:02:23.38 SS.reconfigure :exhale @18:02:23.42 SS.hold @18:02:47.92 Intake.select :close @18:02:48.08 SSV.dial :exhaust @18:02:48.84 Exhaust.select :open @18:02:48.95 SS.seek 0ml @18:02:57.71 Exhaust.select :close @18:02:57.82 SSV.dial CBV @18:02:57.84
CBV.dial SSV @18:02:58.61 Allowing 130s for 25.0ml of remaining 625.0ml @18:02:58.62 Intake.select :open @18:03:00.78 Intake @14.3psia, vacuum from 8.8 to 11.8psi @18:03:00.80 SS.reconfigure :inhaling 0.47ml/s @18:03:00.85 SS.seek 25ml @18:03:54.97 Vacuum too high -- waiting up to 40 seconds @18:03:54.98 SS.reconfigure :exhale @18:03:55.04 SS.hold @18:04:11.45 Intake @14.5psia, vacuum from 9.0 to 12.0psi @18:04:11.47 SS.reconfigure :inhaling 0.38ml/s @18:04:11.51 SS.seek 25ml @18:04:14.41 Stroke completed -- waiting for pressure to equalize ... @18:04:14.42 SS.reconfigure :exhale @18:04:14.47 SS.hold @18:04:29.13 Intake.select :close @18:04:29.28 SSV.dial :exhaust @18:04:30.04 Exhaust.select :open @18:04:30.15 SS.seek 0ml @18:04:38.98 Exhaust.select :close @18:04:39.09 SSV.dial CBV @18:04:39.12
CBV.dial SSV @18:04:39.87 Allowing 130s for 25.0ml of remaining 600.0ml @18:04:39.88 Intake.select :open @18:04:42.05 Intake @14.4psia, vacuum from 8.9 to 11.9psi @18:04:42.06 SS.reconfigure :inhaling 0.38ml/s @18:04:42.11 SS.seek 25ml @18:05:53.25 Stroke completed -- waiting for pressure to equalize ... @18:05:53.26 SS.reconfigure :exhale @18:05:53.31 SS.hold @18:06:23.85 Intake.select :close @18:06:24.01 SSV.dial :exhaust @18:06:24.76 Exhaust.select :open @18:06:24.87 SS.seek 0ml @18:06:33.68 Exhaust.select :close @18:06:33.79 SSV.dial CBV @18:06:33.81
CBV.dial SSV @18:06:34.57 Allowing 130s for 25.0ml of remaining 575.0ml @18:06:34.58 Intake.select :open @18:06:36.75 Intake @14.3psia, vacuum from 8.8 to 11.8psi @18:06:36.76 SS.reconfigure :inhaling 0.38ml/s @18:06:36.81 SS.seek 25ml @18:07:44.59 Vacuum too high -- waiting up to 40 seconds @18:07:44.60 SS.reconfigure :exhale @18:07:44.65 SS.hold @18:08:03.74 Intake @14.1psia, vacuum from 8.6 to 11.6psi @18:08:03.76 SS.reconfigure :inhaling 0.31ml/s @18:08:03.81 SS.seek 25ml @18:08:08.66 Stroke completed -- waiting for pressure to equalize ... @18:08:08.67 SS.reconfigure :exhale @18:08:08.71 SS.hold @18:08:29.16 Intake.select :close @18:08:29.32 SSV.dial :exhaust @18:08:30.07 Exhaust.select :open @18:08:30.18 SS.seek 0ml @18:08:39.22 Exhaust.select :close @18:08:39.33 SSV.dial CBV @18:08:39.35
CBV.dial SSV @18:08:40.11 Allowing 130s for 25.0ml of remaining 550.0ml @18:08:40.12 Intake.select :open @18:08:42.29 Intake @14.4psia, vacuum from 8.9 to 11.9psi @18:08:42.31 SS.reconfigure :inhaling 0.31ml/s @18:08:42.36 SS.seek 25ml @18:10:15.72 Stroke completed -- waiting for pressure to equalize ... @18:10:15.73 SS.reconfigure :exhale @18:10:15.77 SS.hold @18:10:50.13 SS.reconfigure :default @18:10:50.18 SS.hold @18:10:50.25 Intake.select :close @18:10:50.41 ShallowSampler::Clogged in MAIN -- Filtering too Slowly after 475.0ml @18:10:50.42 SSV.dial :exhaust @18:10:51.19 Exhaust.select :open @18:10:51.30 SS.reconfigure :exhale @18:10:51.35 SS.seek 0ml @18:11:00.44 Exhaust.select :close @18:11:00.55 Sampled 475.0ml @18:11:00.56 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @18:11:00.57 Intake.select :close @18:11:00.64 CSV.dial :waste @18:11:00.80 SSV.dial CBV,avoiding: :exhaust @18:11:00.83 CS.seek 0ml @18:11:00.86
CBV.dial SSV @18:11:00.96 CSV.dial :air @18:11:01.70 CS.seek 10ml @18:11:02.74
CTV.dial :air @18:11:03.53 delay 15 seconds @18:11:18.55 SSV.dial :spit,avoiding: :exhaust @18:11:19.86 CTV.dial CSV,avoiding: :intake @18:11:19.90
SS.seek 0ml @18:11:20.03 SSV.dial CBV,avoiding: :exhaust @18:11:26.18 SSV.dial between CBV and plug @18:11:26.68 CBV.dial between SSV and waste2 @18:11:27.09 CTV.dial CSV,avoiding: :air @18:11:27.12
CSV.dial CTV @18:11:27.64 CS.reconfigure :pressurize @18:11:27.69 CS.seek 0ml @18:11:46.50 BubblePoint: Intake @24.3psia, Outlet @0.3psia @18:11:46.51 CBV.dial SSV @18:11:47.21 SS.reconfigure :default @18:11:47.33 SS.seek 10ml @18:11:52.89 delay 15 seconds @18:12:08.03 Evacuated: Intake @23.9psia, Outlet @24.9psia @18:12:08.04 SSV.dial :spit,avoiding: :exhaust @18:12:09.07 SS.reconfigure :exhale @18:12:09.12 SS.seek 0ml @18:12:14.17 SS.reconfigure :fast @18:12:14.21 SS.seek 5ml @18:12:17.02 SSV.dial CBV,avoiding: :exhaust @18:12:17.05
CBV.dial SSV @18:12:18.39 CTV.dial :air,avoiding: :intake @18:12:19.73 CSV.dial CTV @18:12:19.78
SS.seek 10ml @18:12:20.94 CSV.dial :air @18:12:21.37 CS.reconfigure :fast @18:12:21.42 CS.seek 0ml @18:12:22.28
SSV.dial :spit,avoiding: :exhaust @18:12:23.61 SS.reconfigure :exhale @18:12:23.66 SS.seek 0ml @18:12:28.65 SS.reconfigure :fast @18:12:28.70 SS.seek 5ml @18:12:28.77 CS.stop @18:12:31.50 SSV.dial CBV,avoiding: :exhaust @18:12:31.53
CBV.dial SSV @18:12:33.87 SSV.dial :spit,avoiding: :exhaust @18:12:35.21 SS.reconfigure :default @18:12:35.25 SS.seek 0ml @18:12:38.51 SS.stop @18:12:38.55 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @18:12:38.56 Intake.select :close @18:12:38.63 CSV.dial :waste @18:12:38.76 SSV.dial CBV,avoiding: :exhaust @18:12:38.79
CBV.dial SSV @18:12:39.43 CS.seek 0ml @18:12:39.50 CSV.dial :air @18:12:40.11
CTV.dial :air @18:12:40.23 CS.seek 10ml @18:12:40.30
delay 15 seconds @18:12:55.32 SSV.dial :spit,avoiding: :exhaust @18:12:56.64 CTV.dial CSV,avoiding: :intake @18:12:56.68
SS.reconfigure :exhale @18:12:56.78 SS.seek 0ml @18:12:57.22 SSV.dial CBV,avoiding: :exhaust @18:13:04.62 SSV.dial between CBV and plug @18:13:05.09 CBV.dial between SSV and waste2 @18:13:05.50 CSV.dial between air and CTV @18:13:06.00 CS.seek 7.958ml @18:13:11.47 CTV.dial CSV,avoiding: :air @18:13:11.49
CSV.dial CTV @18:13:11.95 CS.reconfigure :pressurize @18:13:12.00 CS.seek 0ml @18:13:28.81 BubblePoint: Intake @24.3psia, Outlet @3.6psia @18:13:28.82 CBV.dial SSV @18:13:29.15 SS.reconfigure :default @18:13:29.20 SS.seek 10ml @18:13:34.95 delay 15 seconds @18:13:50.09 Evacuated: Intake @24.1psia, Outlet @25.0psia @18:13:50.10 SSV.dial :spit,avoiding: :exhaust @18:13:51.13 SS.reconfigure :exhale @18:13:51.18 SS.seek 0ml @18:13:56.16 SS.reconfigure :fast @18:13:56.21 SS.seek 5ml @18:13:59.22 SSV.dial CBV,avoiding: :exhaust @18:13:59.25
CBV.dial SSV @18:14:00.58 CTV.dial :air,avoiding: :intake @18:14:01.93 CSV.dial CTV @18:14:01.97
SS.seek 10ml @18:14:03.09 CSV.dial :air @18:14:03.53 CS.reconfigure :fast @18:14:03.58 CS.seek 0ml @18:14:04.65
SSV.dial :spit,avoiding: :exhaust @18:14:05.99 SS.reconfigure :exhale @18:14:06.03 SS.seek 0ml @18:14:08.08 CS.stop @18:14:10.98
SS.reconfigure :fast @18:14:11.02 SS.seek 5ml @18:14:13.96 SSV.dial CBV,avoiding: :exhaust @18:14:13.99
CBV.dial SSV @18:14:16.32 SSV.dial :spit,avoiding: :exhaust @18:14:17.63 SS.reconfigure :default @18:14:17.67 SS.seek 0ml @18:14:20.76 SS.stop @18:14:20.80 Lysing: Adding 2.0ml AqMeOH @18:14:20.81 CBV.dial between CSV and waste1,avoiding: :DBV @18:14:22.30 CTV.dial CSV @18:14:23.63 CSV.dial CSR @18:14:24.42 Pulling 1ml of CSR.AqMeOH @18:14:24.43 CSR.select :AqMeOH @18:14:24.55 CS.reconfigure :default @18:14:24.60 CS.seek 1ml @18:14:28.18 delay 5 seconds @18:14:33.20 CSR.select :air @18:14:33.37 CS.seek 6ml @18:14:47.43 delay 2 seconds @18:14:49.44 CSV.dial :waste2 @18:14:50.01 CS.reconfigure :fast @18:14:50.06 CS.seek 0ml @18:15:04.36 CSV.dial CSR @18:15:04.80 Pulling 2ml of CSR.AqMeOH @18:15:04.81 CSR.select :AqMeOH @18:15:04.93 CS.reconfigure :default @18:15:04.98 CS.seek 2ml @18:15:11.12 delay 5 seconds @18:15:16.13 CSR.select :air @18:15:16.31 CS.seek 5ml @18:15:24.96 delay 2 seconds @18:15:26.98 CSV.dial CTV @18:15:27.42 CS.seek 1ml @18:15:38.63 Before lysing Collection Puck Heater at 17.4C/28.6psia @18:15:38.65 Heating Puck.from 2, :sh1 in Collection Clamp to 70C for 10:00 @18:15:38.67 CH.seek 70C,8:00 @18:17:44.00 CH.hold 10:00,70C @18:17:44.20 Begun lysing Collection Puck Heater at 68.1C/34.9psia @18:27:44.22 Ended lysing Collection Puck Heater at 70C/34.2psia @18:27:44.23 CS.seek 0ml @18:27:47.61 CTV.dial between spare and air,avoiding: :intake @18:27:48.67 CSV.dial :air @18:27:49.12 CS.seek 5ml @18:28:02.94 CSV.dial CTV @18:28:03.40 delay 2 seconds @18:28:05.42 CSV.dial :air @18:28:05.87 delay 2 seconds @18:28:07.89 CSV.dial CTV @18:28:08.34 delay 2 seconds @18:28:10.35 CSV.dial :air @18:28:10.81 delay 2 seconds @18:28:12.83 CS.seek 0ml @18:28:26.47 Puck @77.7psia after final lysis pressurization @18:28:26.48 Recovering Lysate into Collection syringe @18:28:26.49 CSV.dial CBV @18:28:27.51 CBV.dial CSV @18:28:27.90 CS.seek 5ml @18:28:41.60 delay 5 seconds @18:28:46.62 CTV.dial :air @18:28:47.15 CS.seek 7.001ml @18:28:53.13 delay 5 seconds @18:28:58.15 CSV.dial :air2 @18:28:58.59 CS.seek 10ml @18:29:07.04 CSV.dial between air and CTV,avoiding: CBV @18:29:08.18 Lysing Complete @18:29:08.25 Storing sh1 puck in Collection Clamp to tube 1 @18:29:08.87 SC.reconfigure :tight @18:29:08.92 SC.seek 1 @18:29:12.52 SC.configure :limp @18:29:12.73 Elbow.reconfigure :tight @18:29:12.78 Elbow.seek 1 @18:29:14.56 Forearm.reconfigure :tight @18:29:14.60 Forearm.seek 1 + 450 counts @18:29:15.61 SE.reconfigure :pick @18:29:15.67 SE.seek :beamBreak @18:29:18.33 SE.configure :default @18:29:18.49 Elbow.reconfigure :limp @18:29:18.54 Forearm.reconfigure :loose @18:29:18.59 Forearm.seek 1 @18:29:18.89 Elbow.hold @18:29:18.96 Elbow.reconfigure :tight @18:29:19.06 SE.seek 7.1 Pucks below Top Plate @18:29:19.67 Forearm.force -20 @18:29:19.71 Forearm.seek 1 + 264 counts @18:29:20.00 Forearm.reconfigure :tight @18:29:20.05 Forearm.seek 1 + 450 counts @18:29:20.34 SE.seek 8.1 Pucks below Top Plate @18:29:20.87 Forearm.reconfigure :loose @18:29:20.92 Forearm.seek :retract @18:29:21.90 Elbow.seek CC @18:29:23.61 Elbow.reconfigure :loose @18:29:23.71 Forearm.seek CC @18:29:24.72 Hand.close @18:29:25.38 CC.seek :open @18:29:30.58 Forearm.seek :retract @18:29:31.55 Elbow.reconfigure :tight @18:29:31.60 Elbow.seek 1 @18:29:33.39 Elbow.reconfigure :limp @18:29:33.44 Forearm.seek 1 @18:29:34.49 Elbow.hold @18:29:34.55 Elbow.reconfigure :tight @18:29:34.60 Hand.open @18:29:35.37 Forearm.force -20 @18:29:35.41 Forearm.seek 1 + 318 counts @18:29:35.72 Forearm.reconfigure :tight @18:29:35.76 Forearm.seek 1 + 450 counts @18:29:36.03 SE.seek :down @18:29:37.17
Forearm.reconfigure :loose @18:29:37.21 Forearm.seek :retract @18:29:38.19 SE.stop @18:29:38.24
Tranferring DA @18:29:38.25 Beginning samplePassC @18:29:38.26 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @18:29:38.46 Elbow.seek FlushPuck::Garage @18:29:40.28 Elbow.reconfigure :loose @18:29:40.39 Forearm.seek :garage @18:29:41.41 Hand.close @18:29:42.12 Forearm.seek :retract @18:29:43.16 Elbow.reconfigure :tight @18:29:43.21 Elbow.seek CC @18:29:46.18 Elbow.reconfigure :loose @18:29:46.29 Forearm.reconfigure :tight @18:29:46.33 Forearm.seek CC @18:29:48.07 CC.seek :closed @18:29:52.60 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.42 @18:29:52.67 Hand.open @18:29:53.32 Forearm.reconfigure :loose @18:29:53.36 Forearm.seek :retract @18:29:54.34 PSV.dial PSR @18:29:55.71 Pulling 1.5ml of PSR.flush @18:29:55.72 PSR.select :flush @18:29:55.84 PS.seek 1.5ml @18:30:12.01 delay 5 seconds @18:30:17.03 PSV.dial :waste1 @18:30:17.50 PS.seek 0ml @18:30:33.61 PSV.dial PSR @18:30:34.08 PSR.select :air @18:30:34.19 PS.seek 2.5ml @18:31:00.52 PRV.dial CBV,avoiding: :ab2 @18:31:00.54
CBV.dial PRV @18:31:02.29 PSV.dial :mixloop @18:31:03.33 CTV.dial :air,avoiding: :intake @18:31:03.34 PTV.dial :PRVmixing @18:31:04.68 PS.seek 0ml @18:31:31.10 PSV.dial :air @18:31:31.58 PS.seek 2.5ml @18:31:57.92 PSV.dial :mixloop @18:31:58.39 PS.seek 0ml @18:32:24.73 PSV.dial :air @18:32:25.21 PS.seek 2.5ml @18:32:51.57 PSV.dial :mixloop @18:32:52.05 PS.seek 0ml @18:33:18.36 Garaging FlushPuck in Collection Clamp @18:33:18.42 Elbow.reconfigure :tight @18:33:18.47 Elbow.seek CC @18:33:19.13 Elbow.reconfigure :loose @18:33:19.24 Forearm.seek CC @18:33:20.26 Hand.close @18:33:20.88 CC.seek :open @18:33:25.50 Forearm.seek :retract @18:33:26.46 Elbow.reconfigure :tight @18:33:26.50 Elbow.seek :garage @18:33:29.35 Elbow.reconfigure :loose @18:33:29.45 Forearm.reconfigure :tight @18:33:29.50 Forearm.seek :garage @18:33:30.52 Hand.open @18:33:31.19 Forearm.reconfigure :loose @18:33:31.23 Forearm.seek :retract @18:33:32.22 PSV.dial :mixloop @18:33:32.29 PTV.dial :PRVmixing @18:33:32.37 PRV.dial CBV @18:33:32.40
CBV.dial PRV @18:33:32.55 CTV.dial :air,avoiding: :intake @18:33:32.57 Loading lyfil puck from tube 2 into Collection Clamp @18:33:32.80 SC.reconfigure :tight @18:33:32.85 SC.seek 2 @18:33:36.43 SC.configure :limp @18:33:36.65 Elbow.reconfigure :tight @18:33:36.70 Elbow.seek 2 @18:33:38.59 Forearm.reconfigure :tight @18:33:38.64 Forearm.seek 2 + 450 counts @18:33:39.67 SE.reconfigure :pick @18:33:39.73 SE.seek :beamBreak @18:33:41.39 SE.configure :default @18:33:41.99 Elbow.seek 2 @18:33:42.18 Forearm.seek 2 + 300 counts @18:33:42.45 Forearm.reconfigure :loose @18:33:42.50 Hand.close @18:33:43.14 SE.seek :down @18:33:43.35
Forearm.seek :retract @18:33:44.27 Elbow.seek CC @18:33:44.38 SE.stop @18:33:45.85
Elbow.reconfigure :loose @18:33:45.95 Forearm.reconfigure :tight @18:33:46.00 Forearm.seek CC @18:33:47.24 CC.seek :closed @18:33:51.76 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.19 @18:33:51.77 Hand.open @18:33:52.44 Forearm.reconfigure :loose @18:33:52.49 Forearm.seek :retract @18:33:53.44 CTV.dial CSV,avoiding: :intake @18:33:54.80 CSV.dial CTV @18:33:55.24 PS.seek 2.5ml @18:34:21.76 CS.seek 4.5ml @18:34:36.60 delay 8 seconds @18:34:44.62 PSV.dial :air @18:34:45.09 delay 2 seconds @18:34:47.11 PSV.dial :waste2 @18:34:47.84 PS.seek 0ml @18:35:14.16 CBV.dial between PRV and waste1 @18:35:14.68 CSV.dial :air @18:35:15.14 CS.seek 10ml @18:35:29.97 CSV.dial CTV @18:35:30.43 CS.seek 0ml @18:35:56.84 CBV.dial :waste1 @18:35:57.16 delay 5 seconds @18:36:02.18 CTV.dial :air,avoiding: :intake @18:36:03.61 Storing lyfil puck in Collection Clamp to tube 1 @18:36:03.79 SC.reconfigure :tight @18:36:03.84 SC.seek 1 @18:36:07.47 SC.configure :limp @18:36:07.68 Elbow.reconfigure :tight @18:36:07.73 Elbow.seek 1 @18:36:09.56 Forearm.reconfigure :tight @18:36:09.60 Forearm.seek 1 + 450 counts @18:36:10.75 SE.reconfigure :pick @18:36:10.85 SE.seek :beamBreak @18:36:13.42 SE.configure :default @18:36:13.58 Elbow.reconfigure :limp @18:36:13.63 Forearm.reconfigure :loose @18:36:13.67 Forearm.seek 1 @18:36:13.98 Elbow.hold @18:36:14.05 Elbow.reconfigure :tight @18:36:14.15 SE.seek 8.1 Pucks below Top Plate @18:36:14.62 Forearm.force -20 @18:36:14.66 Forearm.seek 1 + 306 counts @18:36:14.96 Forearm.reconfigure :tight @18:36:15.01 Forearm.seek 1 + 450 counts @18:36:15.21 SE.seek 9.1 Pucks below Top Plate @18:36:16.13 Forearm.reconfigure :loose @18:36:16.24 Forearm.seek :retract @18:36:17.19 Elbow.seek CC @18:36:18.89 Elbow.reconfigure :loose @18:36:18.99 Forearm.seek CC @18:36:20.00 Hand.close @18:36:20.62 CC.seek :open @18:36:25.37 Forearm.seek :retract @18:36:26.36 Elbow.reconfigure :tight @18:36:26.41 Elbow.seek 1 @18:36:28.11 Elbow.reconfigure :limp @18:36:28.17 Forearm.seek 1 @18:36:29.17 Elbow.hold @18:36:29.24 Elbow.reconfigure :tight @18:36:29.29 Hand.open @18:36:29.99 Forearm.force -20 @18:36:30.04 Forearm.seek 1 + 320 counts @18:36:30.33 Forearm.reconfigure :tight @18:36:30.38 Forearm.seek 1 + 450 counts @18:36:30.62 SE.seek :down @18:36:31.76
Forearm.reconfigure :loose @18:36:31.80 Forearm.seek :retract @18:36:32.71 SE.stop @18:36:32.77
PSV.dial :air @18:36:33.53 PS.seek 2.5ml @18:36:59.88 delay 1 second @18:37:00.90 PSV.dial :mixloop @18:37:01.35 delay 1 second @18:37:02.37 PSV.dial :air @18:37:02.84 delay 1 second @18:37:03.85 PSV.dial :mixloop @18:37:04.38 delay 1 second @18:37:05.40 PSV.dial :air @18:37:05.87 delay 1 second @18:37:06.89 PS.seek 0ml @18:37:33.26 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @18:37:33.45 Elbow.seek FlushPuck::Garage @18:37:35.27 Elbow.reconfigure :loose @18:37:35.37 Forearm.seek :garage @18:37:36.32 Hand.close @18:37:37.12 Forearm.seek :retract @18:37:38.08 Elbow.reconfigure :tight @18:37:38.12 Elbow.seek PC @18:37:39.38 Elbow.reconfigure :loose @18:37:39.48 Forearm.reconfigure :tight @18:37:39.53 Forearm.seek PC @18:37:40.81 PC.seek :closed @18:37:45.42 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.60 @18:37:45.48 Hand.open @18:37:46.18 Forearm.reconfigure :loose @18:37:46.23 Forearm.seek :retract @18:37:47.18 PRV.dial :air @18:37:47.63 PSV.dial :mixloop @18:37:48.16 PS.seek 0.3ml @18:37:52.10 PSV.dial PSR @18:37:53.15 Pulling 0.4ml of PSR.air @18:37:53.59 PSR.select :air @18:37:53.63 PS.seek 0.7ml @18:37:58.51 delay 3 seconds @18:38:01.52 PSR.select :air @18:38:01.54 PS.seek 2.5ml @18:38:20.79 delay 3 seconds @18:38:23.80 PSV.dial :mixloop @18:38:24.85 PTV.dial :mixing @18:38:25.60 PBV.dial :waste2 @18:38:26.56 PS.seek 0ml @18:38:52.97 Garaging FlushPuck in Processing Clamp @18:38:53.05 Elbow.reconfigure :tight @18:38:53.09 Elbow.seek PC @18:38:53.21 Elbow.reconfigure :loose @18:38:53.31 Forearm.seek PC @18:38:54.26 Hand.close @18:38:54.87 PC.seek :open @18:38:59.59 Forearm.seek :retract @18:39:00.54 Elbow.reconfigure :tight @18:39:00.59 Elbow.seek :garage @18:39:01.91 Elbow.reconfigure :loose @18:39:02.01 Forearm.reconfigure :tight @18:39:02.06 Forearm.seek :garage @18:39:03.02 Hand.open @18:39:03.68 Forearm.reconfigure :loose @18:39:03.73 Forearm.seek :retract @18:39:04.76 Loading da puck from tube 2 into Processing Clamp @18:39:04.99 SC.reconfigure :tight @18:39:05.04 SC.seek 2 @18:39:08.63 SC.configure :limp @18:39:08.85 Elbow.reconfigure :tight @18:39:08.89 Elbow.seek 2 @18:39:10.73 Forearm.reconfigure :tight @18:39:10.77 Forearm.seek 2 + 450 counts @18:39:11.92 SE.reconfigure :pick @18:39:12.40 SE.seek :beamBreak @18:39:13.99 SE.configure :default @18:39:14.15 Elbow.seek 2 @18:39:14.34 Forearm.seek 2 + 300 counts @18:39:14.57 Forearm.reconfigure :loose @18:39:14.62 Hand.close @18:39:15.26 SE.seek :down @18:39:15.46
Forearm.seek :retract @18:39:16.42 Elbow.seek PC @18:39:16.53 SE.stop @18:39:18.94
Elbow.reconfigure :loose @18:39:19.05 Forearm.reconfigure :tight @18:39:19.09 Forearm.seek PC @18:39:20.37 PC.seek :closed @18:39:25.04 Processing Clamp onSpring/offSpring for 16/26 samples, on/off torque 1.65 @18:39:25.05 Hand.open @18:39:25.68 Forearm.reconfigure :loose @18:39:25.72 Forearm.seek :retract @18:39:26.71 Heating Puck.from 2, :da in Processing Clamp to 28C for 2:00:00 @18:39:26.73 PH.seek 28C,5:00 @18:40:02.08 PH.hold 2:00:00,28C @18:40:02.12 delay 1:30 @18:41:32.17 PSR.select :air @18:41:32.19 PSV.dial PSR @18:41:33.24 Pulling 0.5ml of PSR.wash PSR.select :wash @18:41:33.36 PS.seek 0.5ml @18:41:39.38 delay 5 seconds @18:41:44.39 PSR.select :air @18:41:44.52 PS.seek 2ml @18:42:00.62 PTV.dial :puck @18:42:01.36 PSV.dial PTV @18:42:02.13 PBV.dial :waste1 @18:42:03.52 PS.seek 1.5ml @18:42:09.44 delay 3 seconds @18:42:12.46 PBV.dial between PSV and waste1 @18:42:12.82 PS.seek 0ml @18:42:28.97 delay 5:00 @18:47:28.99 recoverPSRreagent08 @18:47:29.00 PTV.dial :puck @18:47:29.01 PBV.dial :waste1 @18:47:29.39 delay 30 seconds @18:47:59.41 PBV.dial between PSV and waste1 @18:47:59.77 PSR.select :air @18:47:59.79 PSV.dial PSR @18:48:00.67 PS.reconfigure :fast @18:48:00.72 PS.seek 2.5ml @18:48:24.80 PSV.dial PTV @18:48:25.54 delay 5 seconds @18:48:30.56 PSV.dial PSR @18:48:31.31 delay 5 seconds @18:48:36.33 PSV.dial PTV @18:48:37.07 delay 5 seconds @18:48:42.09 PSV.dial :air @18:48:43.41 PS.seek 0ml @18:49:06.71 PTV.dial :PRVmixing,via: PRV @18:49:08.03 PSV.dial :mixloop @18:49:08.48 PRV.dial :ab1 @18:49:09.23 PS.reconfigure :slow @18:49:09.28 PS.seek 0.15ml @18:49:12.77 delay 2 seconds @18:49:14.79 CBV.dial PRV @18:49:14.82
PRV.dial CBV @18:49:15.39 PS.seek 0.35ml @18:49:20.02 delay 2 seconds @18:49:22.04 CBV.dial between PRV and PSV @18:49:22.36 PRV.dial :air @18:49:22.81 PS.seek 0.9ml @18:49:34.46 PRV.dial :waste2 @18:49:35.20 PS.seek 0.5ml @18:49:43.87 addPMLReagent08 @18:49:43.88 PSV.dial :air @18:49:44.34 PS.reconfigure :default @18:49:44.39 PS.seek 2ml @18:50:00.61 PSV.dial :PML @18:50:01.08 PTV.dial :mixing @18:50:01.83 PBV.dial :waste1 @18:50:02.26 PS.seek 1.85ml @18:50:04.58 PBV.dial between PSV and waste1 @18:50:04.95 PS.seek 0ml @18:50:24.74 delay 2 seconds @18:50:26.76 Beginning samplePassC @18:50:26.77 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @18:50:26.95 Elbow.reconfigure :tight @18:50:27.00 Elbow.seek FlushPuck::Garage @18:50:28.37 Elbow.reconfigure :loose @18:50:28.47 Forearm.seek :garage @18:50:29.44 Hand.close @18:50:30.23 Forearm.seek :retract @18:50:31.19 Elbow.reconfigure :tight @18:50:31.24 Elbow.seek CC @18:50:34.21 Elbow.reconfigure :loose @18:50:34.75 Forearm.reconfigure :tight @18:50:34.80 Forearm.seek CC @18:50:36.07 CC.seek :closed @18:50:40.64 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.43 @18:50:40.75 Hand.open @18:50:41.42 Forearm.reconfigure :loose @18:50:41.47 Forearm.seek :retract @18:50:42.45 PSV.dial PSR @18:50:43.62 Pulling 1.5ml of PSR.flush PSR.select :flush @18:50:43.74 PS.seek 1.5ml @18:50:59.93 delay 5 seconds @18:51:04.95 PSV.dial :waste1 @18:51:05.43 PS.seek 0ml @18:51:21.55 PSV.dial PSR @18:51:22.02 PSR.select :air @18:51:22.14 PS.seek 2.5ml @18:51:48.77 PRV.dial CBV,avoiding: :ab2 @18:51:48.80
CBV.dial PRV @18:51:50.43 PSV.dial :mixloop @18:51:51.46 CTV.dial :air,avoiding: :intake @18:51:51.53 PTV.dial :PRVmixing @18:51:52.31 PS.seek 0ml @18:52:18.58 PSV.dial :air @18:52:19.06 PS.seek 2.5ml @18:52:45.58 PSV.dial :mixloop @18:52:46.06 PS.seek 0ml @18:53:12.42 PSV.dial :air @18:53:12.90 PS.seek 2.5ml @18:53:39.30 PSV.dial :mixloop @18:53:39.77 PS.seek 0ml @18:54:06.65 Garaging FlushPuck in Collection Clamp @18:54:06.72 Elbow.reconfigure :tight @18:54:06.77 Elbow.seek CC @18:54:06.89 Elbow.reconfigure :loose @18:54:07.00 Forearm.seek CC @18:54:07.98 Hand.close @18:54:08.61 CC.seek :open @18:54:13.23 Forearm.seek :retract @18:54:14.19 Elbow.reconfigure :tight @18:54:14.24 Elbow.seek :garage @18:54:17.16 Elbow.reconfigure :loose @18:54:17.27 Forearm.reconfigure :tight @18:54:17.31 Forearm.seek :garage @18:54:18.25 Hand.open @18:54:18.92 Forearm.reconfigure :loose @18:54:18.96 Forearm.seek :retract @18:54:20.00 delayUntil "18:58:26.75" @18:54:20.03 Whole Cell Archive sampling 1000ml @18:54:20.05 Loading wcr puck from tube 2 into Collection Clamp @18:54:20.29 SC.reconfigure :tight @18:54:20.34 SC.seek 2 @18:54:20.68 SC.configure :limp @18:54:20.90 Elbow.reconfigure :tight @18:54:20.96 Elbow.seek 2 @18:54:22.90 Forearm.reconfigure :tight @18:54:22.95 Forearm.seek 2 + 450 counts @18:54:24.04 SE.reconfigure :pick @18:54:24.10 SE.seek :beamBreak @18:54:25.79 SE.configure :default @18:54:26.40 Elbow.seek 2 @18:54:26.60 Forearm.seek 2 + 300 counts @18:54:26.83 Forearm.reconfigure :loose @18:54:26.88 Hand.close @18:54:27.50 SE.seek :down @18:54:27.70 Forearm.seek :retract @18:54:28.66 Elbow.seek CC @18:54:28.85 SE.stop @18:54:30.24 Elbow.reconfigure :loose @18:54:30.35 Forearm.reconfigure :tight @18:54:30.40 Forearm.seek CC @18:54:31.61 CC.seek :closed @18:54:36.15 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.15 @18:54:36.17 Hand.open @18:54:36.80 Forearm.reconfigure :loose @18:54:36.85 Forearm.seek :retract @18:54:37.83 collectSample 1000ml w/bubble point of 35psi @18:54:37.84 Priming 7ml @18:54:37.85 Intake.select :close @18:54:37.86 Exhaust.select :close @18:54:37.88 SS.hold @18:54:38.08 Calibrating pressure sensors @18:54:38.09 CTV.dial :air @18:54:38.17 SSV.dial :spit,avoiding: :exhaust @18:54:38.27 SS.reconfigure :fast @18:54:38.32 SS.seek 5ml @18:54:41.45 SSV.dial CBV @18:54:41.48 CBV.dial SSV @18:54:42.84 delay 3.8 seconds @18:54:46.79 Sensor limits: Intake max 118.3psia; Outlet max 110.7psia @18:54:46.80 SSV.dial :spit @18:54:48.13 SS.seek 0ml @18:54:50.95 SSV.dial CBV @18:54:52.28 CTV.dial :intake @18:54:53.05 SSV.dial CBV @18:54:53.09 CBV.dial SSV @18:54:53.21 Allowing 40s for 7.0ml of remaining 7.0ml @18:54:53.22 Intake.select :open @18:54:55.39 Intake @14.7psia, vacuum from 9.2 to 12.2psi @18:54:55.41 SS.reconfigure :inhaling 2.20ml/s @18:54:55.46 SS.seek 7ml @18:55:02.02 Stroke completed -- waiting for pressure to equalize ... @18:55:02.04 SS.reconfigure :exhale @18:55:02.08 SS.hold @18:55:10.65 Intake.select :close @18:55:11.18 SSV.dial :exhaust @18:55:12.01 Exhaust.select :open @18:55:12.12 SS.seek 0ml @18:55:16.07 Exhaust.select :close @18:55:16.19 Sampling 1000.0ml @18:55:16.20 Intake.select :close @18:55:16.21 Exhaust.select :close @18:55:16.22 SS.reconfigure :default @18:55:16.27 SS.hold @18:55:16.48 CTV.dial :intake @18:55:16.56 SSV.dial CBV @18:55:16.59 CBV.dial SSV @18:55:17.38 Allowing 130s for 25.0ml of remaining 1000.0ml @18:55:17.40 Intake.select :open @18:55:19.56 Intake @15.4psia, vacuum from 9.9 to 12.9psi @18:55:19.58 SS.reconfigure :inhaling 2.20ml/s @18:55:19.63 SS.seek 25ml @18:55:30.41 Vacuum too high -- waiting up to 40 seconds @18:55:30.42 SS.reconfigure :exhale @18:55:30.48 SS.hold @18:55:37.38 Intake @15.6psia, vacuum from 10.0 to 13.0psi @18:55:37.40 SS.reconfigure :inhaling 1.76ml/s @18:55:37.45 SS.seek 25ml @18:55:43.62 Stroke completed -- waiting for pressure to equalize ... @18:55:43.63 SS.reconfigure :exhale @18:55:43.68 SS.hold @18:55:55.31 Intake.select :close @18:55:55.47 SSV.dial :exhaust @18:55:56.24 Exhaust.select :open @18:55:56.36 SS.seek 0ml @18:56:05.23 Exhaust.select :close @18:56:05.34 SSV.dial CBV @18:56:05.38 CBV.dial SSV @18:56:06.13 Allowing 130s for 25.0ml of remaining 975.0ml @18:56:06.15 Intake.select :open @18:56:08.32 Intake @15.7psia, vacuum from 10.0 to 13.0psi @18:56:08.34 SS.reconfigure :inhaling 1.76ml/s @18:56:08.38 SS.seek 25ml @18:56:20.62 Vacuum too high -- waiting up to 40 seconds @18:56:20.64 SS.reconfigure :exhale @18:56:20.69 SS.hold @18:56:25.79 Intake @15.1psia, vacuum from 9.6 to 12.6psi @18:56:25.81 SS.reconfigure :inhaling 1.41ml/s @18:56:25.86 SS.seek 25ml @18:56:32.80 Stroke completed -- waiting for pressure to equalize ... @18:56:32.82 SS.reconfigure :exhale @18:56:32.86 SS.hold @18:56:44.47 Intake.select :close @18:56:44.64 SSV.dial :exhaust @18:56:45.40 Exhaust.select :open @18:56:45.51 SS.seek 0ml @18:56:54.54 Exhaust.select :close @18:56:54.65 SSV.dial CBV @18:56:54.69 CBV.dial SSV @18:56:55.43 Allowing 130s for 25.0ml of remaining 950.0ml @18:56:55.44 Intake.select :open @18:56:57.61 Intake @15.8psia, vacuum from 10.0 to 13.0psi @18:56:57.63 SS.reconfigure :inhaling 1.41ml/s @18:56:57.68 SS.seek 25ml @18:57:13.59 Vacuum too high -- waiting up to 40 seconds @18:57:13.61 SS.reconfigure :exhale @18:57:13.66 SS.hold @18:57:18.74 Intake @15.0psia, vacuum from 9.5 to 12.5psi @18:57:18.76 SS.reconfigure :inhaling 1.13ml/s @18:57:18.81 SS.seek 25ml @18:57:24.74 Stroke completed -- waiting for pressure to equalize ... @18:57:24.75 SS.reconfigure :exhale @18:57:24.80 SS.hold @18:57:35.06 Intake.select :close @18:57:35.21 SSV.dial :exhaust @18:57:35.98 Exhaust.select :open @18:57:36.09 SS.seek 0ml @18:57:45.11 Exhaust.select :close @18:57:45.23 SSV.dial CBV @18:57:45.25 CBV.dial SSV @18:57:46.05 Allowing 130s for 25.0ml of remaining 925.0ml @18:57:46.07 Intake.select :open @18:57:48.23 Intake @15.8psia, vacuum from 10.0 to 13.0psi @18:57:48.25 SS.reconfigure :inhaling 1.13ml/s @18:57:48.30 SS.seek 25ml @18:58:12.55 Stroke completed -- waiting for pressure to equalize ... @18:58:12.56 SS.reconfigure :exhale @18:58:12.61 SS.hold @18:58:25.45 Intake.select :close @18:58:25.62 SSV.dial :exhaust @18:58:26.38 Exhaust.select :open @18:58:26.50 SS.seek 0ml @18:58:26.76 recoverPRVreagent08 @18:58:26.77 PTV.dial :mixing @18:58:27.51 PBV.dial :waste1 @18:58:27.89 delay 30 seconds @18:58:35.49 Exhaust.select :close @18:58:35.60 SSV.dial CBV @18:58:35.64 CBV.dial SSV @18:58:36.40 Allowing 130s for 25.0ml of remaining 900.0ml @18:58:36.41 Intake.select :open @18:58:38.58 Intake @15.8psia, vacuum from 10.0 to 13.0psi @18:58:38.60 SS.reconfigure :inhaling 1.13ml/s @18:58:38.65 SS.seek 25ml @18:58:57.91 PBV.dial between PSV and waste1 @18:58:58.29 PSV.dial :air @18:58:58.77 PS.reconfigure :fast @18:58:58.82 PS.seek 2.5ml @18:59:00.09 Vacuum too high -- waiting up to 40 seconds @18:59:00.11 SS.reconfigure :exhale @18:59:00.16 SS.hold @18:59:06.37 Intake @15.7psia, vacuum from 10.0 to 13.0psi @18:59:06.39 SS.reconfigure :inhaling 0.91ml/s @18:59:06.44 SS.seek 25ml @18:59:10.33 Stroke completed -- waiting for pressure to equalize ... @18:59:10.34 SS.reconfigure :exhale @18:59:10.39 SS.hold @18:59:18.33 Intake.select :close @18:59:18.49 SSV.dial :exhaust @18:59:19.27 Exhaust.select :open @18:59:19.39 SS.seek 0ml @18:59:23.20 PSV.dial :mixloop @18:59:23.67 delay 2 seconds @18:59:25.69 PSV.dial :air @18:59:26.28 delay 2 seconds @18:59:28.13 Exhaust.select :close @18:59:28.24 SSV.dial CBV @18:59:28.27 CBV.dial SSV @18:59:28.39 PSV.dial :mixloop @18:59:28.88 delay 2 seconds @18:59:29.05 Allowing 130s for 25.0ml of remaining 875.0ml @18:59:29.07 Intake.select :open @18:59:30.90 PSV.dial :air @18:59:31.23 Intake @15.8psia, vacuum from 10.0 to 13.0psi @18:59:31.25 SS.reconfigure :inhaling 0.91ml/s @18:59:31.30 SS.seek 25ml @18:59:31.38 PS.seek 0ml @18:59:55.07 washCycleDA #1 of 2 @18:59:55.08 PSR.select :air @18:59:55.10 PSV.dial PSR @18:59:56.43 Pulling 0.5ml of PSR.wash @18:59:56.44 PSR.select :wash @18:59:56.56 PS.reconfigure :default @18:59:56.61 PS.seek 0.5ml @19:00:01.39 Stroke completed -- waiting for pressure to equalize ... @19:00:01.41 SS.reconfigure :exhale @19:00:01.45 SS.hold @19:00:02.63 delay 5 seconds @19:00:07.64 PSR.select :air @19:00:07.86 PS.seek 2ml @19:00:14.04 Intake.select :close @19:00:14.20 SSV.dial :exhaust @19:00:14.97 Exhaust.select :open @19:00:15.09 SS.seek 0ml @19:00:24.01 PTV.dial :puck @19:00:24.76 PSV.dial PTV @19:00:24.97 Exhaust.select :close @19:00:25.09 SSV.dial CBV @19:00:25.11 CBV.dial SSV @19:00:25.51 PBV.dial :waste1 @19:00:25.95 Allowing 130s for 25.0ml of remaining 850.0ml @19:00:25.96 Intake.select :open @19:00:26.04 PS.seek 1.5ml @19:00:28.16 Intake @15.6psia, vacuum from 10.0 to 13.0psi @19:00:28.18 SS.reconfigure :inhaling 0.91ml/s @19:00:28.23 SS.seek 25ml @19:00:31.95 delay 3 seconds @19:00:34.97 PBV.dial between PSV and waste1 @19:00:35.34 PS.seek 0ml @19:00:51.51 delay 2:00 @19:00:57.50 Stroke completed -- waiting for pressure to equalize ... @19:00:57.51 SS.reconfigure :exhale @19:00:57.56 SS.hold @19:01:12.43 Intake.select :close @19:01:12.59 SSV.dial :exhaust @19:01:13.36 Exhaust.select :open @19:01:13.48 SS.seek 0ml @19:01:22.30 Exhaust.select :close @19:01:22.41 SSV.dial CBV @19:01:22.45 CBV.dial SSV @19:01:23.21 Allowing 130s for 25.0ml of remaining 825.0ml @19:01:23.22 Intake.select :open @19:01:25.38 Intake @15.6psia, vacuum from 10.0 to 13.0psi @19:01:25.40 SS.reconfigure :inhaling 0.91ml/s @19:01:25.45 SS.seek 25ml @19:01:55.30 Vacuum too high -- waiting up to 40 seconds @19:01:55.32 SS.reconfigure :exhale @19:01:55.37 SS.hold @19:02:05.37 Intake @15.8psia, vacuum from 10.0 to 13.0psi @19:02:05.39 SS.reconfigure :inhaling 0.73ml/s @19:02:05.44 SS.seek 25ml @19:02:05.50 Stroke completed -- waiting for pressure to equalize ... @19:02:05.52 SS.reconfigure :exhale @19:02:05.56 SS.hold @19:02:12.07 Intake.select :close @19:02:12.23 SSV.dial :exhaust @19:02:13.00 Exhaust.select :open @19:02:13.57 SS.seek 0ml @19:02:22.72 Exhaust.select :close @19:02:22.83 SSV.dial CBV @19:02:22.86 CBV.dial SSV @19:02:23.63 Allowing 130s for 25.0ml of remaining 800.0ml @19:02:23.64 Intake.select :open @19:02:25.81 Intake @15.5psia, vacuum from 10.0 to 13.0psi @19:02:25.83 SS.reconfigure :inhaling 0.73ml/s @19:02:25.88 SS.seek 25ml @19:02:51.53 recoverPSRreagent08 @19:02:51.54 PTV.dial :puck @19:02:51.73 PBV.dial :waste1 @19:02:52.10 delay 30 seconds @19:03:01.37 Stroke completed -- waiting for pressure to equalize ... @19:03:01.39 SS.reconfigure :exhale @19:03:01.43 SS.hold @19:03:18.20 Intake.select :close @19:03:18.37 SSV.dial :exhaust @19:03:19.14 Exhaust.select :open @19:03:19.26 SS.seek 0ml @19:03:22.12 PBV.dial between PSV and waste1 @19:03:22.49 PSR.select :air @19:03:22.51 PSV.dial PSR @19:03:23.28 PS.reconfigure :fast @19:03:23.33 PS.seek 2.5ml @19:03:28.49 Exhaust.select :close @19:03:28.61 SSV.dial CBV @19:03:28.64 CBV.dial SSV @19:03:29.41 Allowing 130s for 25.0ml of remaining 775.0ml @19:03:29.42 Intake.select :open @19:03:31.58 Intake @15.4psia, vacuum from 9.9 to 12.9psi @19:03:31.60 SS.reconfigure :inhaling 0.73ml/s @19:03:31.65 SS.seek 25ml @19:03:47.47 PSV.dial PTV @19:03:48.24 delay 5 seconds @19:03:53.26 PSV.dial PSR @19:03:54.02 delay 5 seconds @19:03:59.04 PSV.dial PTV @19:03:59.80 delay 5 seconds @19:04:04.82 PSV.dial :air @19:04:05.61 PS.seek 0ml @19:04:07.07 Stroke completed -- waiting for pressure to equalize ... @19:04:07.09 SS.reconfigure :exhale @19:04:07.14 SS.hold @19:04:29.13 washCycleDA #2 of 2 @19:04:29.14 PSR.select :air @19:04:29.16 PSV.dial PSR @19:04:30.06 Intake.select :close @19:04:30.22 SSV.dial :exhaust @19:04:30.47 Pulling 0.5ml of PSR.wash @19:04:30.49 PSR.select :wash @19:04:30.60 PS.reconfigure :default @19:04:30.65 PS.seek 0.5ml @19:04:30.99 Exhaust.select :open @19:04:31.11 SS.seek 0ml @19:04:36.66 delay 5 seconds @19:04:39.92 Exhaust.select :close @19:04:40.03 SSV.dial CBV @19:04:40.06 CBV.dial SSV @19:04:40.83 Allowing 130s for 25.0ml of remaining 750.0ml @19:04:40.84 Intake.select :open @19:04:41.68 PSR.select :air @19:04:41.80 PS.seek 2ml @19:04:43.00 Intake @15.3psia, vacuum from 9.8 to 12.8psi @19:04:43.02 SS.reconfigure :inhaling 0.73ml/s @19:04:43.07 SS.seek 25ml @19:04:57.91 PTV.dial :puck @19:04:57.99 PSV.dial PTV @19:04:58.74 PBV.dial :waste1 @19:04:59.18 PS.seek 1.5ml @19:05:05.22 delay 3 seconds @19:05:07.77 Vacuum too high -- waiting up to 40 seconds @19:05:07.79 SS.reconfigure :exhale @19:05:07.84 SS.hold @19:05:08.24 PBV.dial between PSV and waste1 @19:05:09.10 PS.seek 0ml @19:05:19.60 Intake @15.7psia, vacuum from 10.0 to 13.0psi @19:05:19.62 SS.reconfigure :inhaling 0.59ml/s @19:05:19.67 SS.seek 25ml @19:05:25.26 delay 2:00 @19:05:34.40 Stroke completed -- waiting for pressure to equalize ... @19:05:34.41 SS.reconfigure :exhale @19:05:34.46 SS.hold @19:05:51.25 Intake.select :close @19:05:51.41 SSV.dial :exhaust @19:05:52.17 Exhaust.select :open @19:05:52.28 SS.seek 0ml @19:06:01.29 Exhaust.select :close @19:06:01.41 SSV.dial CBV @19:06:01.43 CBV.dial SSV @19:06:02.20 Allowing 130s for 25.0ml of remaining 725.0ml @19:06:02.21 Intake.select :open @19:06:04.38 Intake @15.3psia, vacuum from 9.8 to 12.8psi @19:06:04.40 SS.reconfigure :inhaling 0.59ml/s @19:06:04.45 SS.seek 25ml @19:06:39.36 Vacuum too high -- waiting up to 40 seconds @19:06:39.38 SS.reconfigure :exhale @19:06:39.43 SS.hold @19:06:52.37 Intake @15.4psia, vacuum from 9.9 to 12.9psi @19:06:52.39 SS.reconfigure :inhaling 0.47ml/s @19:06:52.44 SS.seek 25ml @19:07:04.62 Stroke completed -- waiting for pressure to equalize ... @19:07:04.63 SS.reconfigure :exhale @19:07:04.68 SS.hold @19:07:23.50 Intake.select :close @19:07:23.66 SSV.dial :exhaust @19:07:24.43 Exhaust.select :open @19:07:24.55 SS.seek 0ml @19:07:25.28 recoverPSRreagent08 @19:07:25.29 PTV.dial :puck @19:07:25.36 PBV.dial :waste1 @19:07:25.74 delay 30 seconds @19:07:33.38 Exhaust.select :close @19:07:33.50 SSV.dial CBV @19:07:33.52 CBV.dial SSV @19:07:34.29 Allowing 130s for 25.0ml of remaining 700.0ml @19:07:34.31 Intake.select :open @19:07:36.47 Intake @15.2psia, vacuum from 9.7 to 12.7psi @19:07:36.49 SS.reconfigure :inhaling 0.47ml/s @19:07:36.54 SS.seek 25ml @19:07:55.76 PBV.dial between PSV and waste1 @19:07:56.13 PSR.select :air @19:07:56.15 PSV.dial PSR @19:07:56.91 PS.reconfigure :fast @19:07:56.96 PS.seek 2.5ml @19:08:21.03 PSV.dial PTV @19:08:21.80 delay 5 seconds @19:08:26.82 PSV.dial PSR @19:08:27.58 delay 5 seconds @19:08:29.80 Vacuum too high -- waiting up to 40 seconds @19:08:29.82 SS.reconfigure :exhale @19:08:29.87 SS.hold @19:08:32.60 PSV.dial PTV @19:08:33.34 delay 5 seconds @19:08:38.36 PSV.dial :air @19:08:39.13 PS.seek 0ml @19:08:48.43 Intake @15.9psia, vacuum from 10.0 to 13.0psi @19:08:48.45 SS.reconfigure :inhaling 0.38ml/s @19:08:48.50 SS.seek 25ml @19:08:52.63 Stroke completed -- waiting for pressure to equalize ... @19:08:52.64 SS.reconfigure :exhale @19:08:52.69 SS.hold @19:09:02.63 getPMLReagent08 @19:09:02.66 PRV.dial :air @19:09:03.11 delay 5 seconds @19:09:07.24 Intake.select :close @19:09:07.41 SSV.dial :exhaust @19:09:08.13 PTV.dial :PRVmixing @19:09:08.18 Exhaust.select :open @19:09:08.32 SS.seek 0ml @19:09:09.47 delay 5 seconds @19:09:14.49 PRV.dial :ab2 @19:09:14.97 PTV.dial :PRVmixing @19:09:15.05 PSV.dial :mixloop @19:09:15.64 PS.reconfigure :default @19:09:15.69 PS.seek 0.3ml @19:09:17.54 Exhaust.select :close @19:09:17.65 SSV.dial CBV @19:09:17.68 CBV.dial SSV @19:09:18.45 Allowing 130s for 25.0ml of remaining 675.0ml @19:09:18.46 Intake.select :open @19:09:19.76 delay 2 seconds @19:09:20.62 Intake @15.4psia, vacuum from 9.9 to 12.9psi @19:09:20.64 SS.reconfigure :inhaling 0.38ml/s @19:09:20.69 SS.seek 25ml @19:09:21.77 PRV.dial :air @19:09:22.29 PS.seek 0.55ml @19:09:25.72 addPMLReagent08 @19:09:25.73 PSV.dial :air @19:09:26.32 PS.seek 2ml @19:09:41.95 PSV.dial :PML @19:09:42.41 PTV.dial :mixing @19:09:43.17 PBV.dial :waste1 @19:09:43.59 PS.seek 1.85ml @19:09:46.06 PBV.dial between PSV and waste1 @19:09:46.43 PS.seek 0ml @19:10:06.15 delay 2 seconds @19:10:08.17 delay 8:00 @19:10:29.63 Stroke completed -- waiting for pressure to equalize ... @19:10:29.64 SS.reconfigure :exhale @19:10:29.69 SS.hold @19:11:08.54 Intake.select :close @19:11:08.70 SSV.dial :exhaust @19:11:09.48 Exhaust.select :open @19:11:09.61 SS.seek 0ml @19:11:18.74 Exhaust.select :close @19:11:18.85 SSV.dial CBV @19:11:18.89 CBV.dial SSV @19:11:19.65 Allowing 130s for 25.0ml of remaining 650.0ml @19:11:19.66 Intake.select :open @19:11:21.82 Intake @15.2psia, vacuum from 9.7 to 12.7psi @19:11:21.84 SS.reconfigure :inhaling 0.38ml/s @19:11:21.89 SS.seek 25ml @19:12:25.20 Vacuum too high -- waiting up to 40 seconds @19:12:25.21 SS.reconfigure :exhale @19:12:25.27 SS.hold @19:12:48.34 Intake @14.9psia, vacuum from 9.4 to 12.4psi @19:12:48.36 SS.reconfigure :inhaling 0.31ml/s @19:12:48.41 SS.seek 25ml @19:12:56.41 Stroke completed -- waiting for pressure to equalize ... @19:12:56.42 SS.reconfigure :exhale @19:12:56.47 SS.hold @19:13:23.08 Intake.select :close @19:13:23.24 SSV.dial :exhaust @19:13:24.01 Exhaust.select :open @19:13:24.13 SS.seek 0ml @19:13:33.14 Exhaust.select :close @19:13:33.26 SSV.dial CBV @19:13:33.29 CBV.dial SSV @19:13:34.05 Allowing 130s for 25.0ml of remaining 625.0ml @19:13:34.06 Intake.select :open @19:13:36.23 Intake @15.2psia, vacuum from 9.7 to 12.7psi @19:13:36.25 SS.reconfigure :inhaling 0.31ml/s @19:13:36.30 SS.seek 25ml @19:15:06.81 Stroke completed -- waiting for pressure to equalize ... @19:15:06.82 SS.reconfigure :exhale @19:15:06.87 SS.hold @19:15:44.16 SS.reconfigure :default @19:15:44.22 SS.hold @19:15:44.29 Intake.select :close @19:15:44.92 ShallowSampler::Clogged in WCR -- Filtering too Slowly after 400.0ml @19:15:44.94 SSV.dial :exhaust @19:15:45.70 Exhaust.select :open @19:15:45.82 SS.reconfigure :exhale @19:15:45.88 SS.seek 0ml @19:15:55.12 Exhaust.select :close @19:15:55.24 Sampled 400.0ml @19:15:55.26 Drying puck before fixing for 5:00 @19:15:55.27 CBV.dial :waste2b @19:15:56.02 delay 1:00 @19:16:56.05 CSV.dial :air @19:16:56.51 CS.reconfigure :fast @19:16:56.56 CS.seek 10ml @19:17:21.06 Pumping wcr puck from tube 2 up to 35psi... @19:17:21.07 CBV.dial SSV @19:17:22.34 CBV.dial between CSV and waste2b @19:17:23.30 CSV.dial between air and CTV @19:17:23.62 CS.seek 7.288ml @19:17:30.65 CTV.dial CSV,avoiding: :air @19:17:30.68 CSV.dial CTV @19:17:31.53 CS.reconfigure :pressurize @19:17:31.58 CS.seek 0ml @19:17:50.71 CSV.dial :air @19:17:51.15 CS.reconfigure :fast @19:17:51.20 CS.seek 10ml @19:18:08.19 recoverPRVreagent08 @19:18:08.20 PTV.dial :mixing @19:18:08.28 PBV.dial :waste1 @19:18:08.66 delay 30 seconds @19:18:15.52 CSV.dial between air and CTV @19:18:15.84 CS.seek 4.141ml @19:18:29.93 CTV.dial CSV,avoiding: :air @19:18:29.96 CSV.dial CTV @19:18:30.35 CS.reconfigure :pressurize @19:18:30.41 CS.seek 0ml @19:18:38.68 PBV.dial between PSV and waste1 @19:18:39.07 PSV.dial :air @19:18:39.54 PS.reconfigure :fast @19:18:39.59 PS.seek 2.5ml @19:18:41.52 CSV.dial :air @19:18:42.43 CS.reconfigure :fast @19:18:42.49 CS.seek 10ml @19:19:03.52 PSV.dial :mixloop @19:19:03.99 delay 2 seconds @19:19:06.01 PSV.dial :air @19:19:06.47 delay 2 seconds @19:19:06.70 CSV.dial between air and CTV @19:19:07.03 CS.seek 4.25ml @19:19:08.49 PSV.dial :mixloop @19:19:08.96 delay 2 seconds @19:19:10.97 PSV.dial :air @19:19:11.45 PS.seek 0ml @19:19:20.81 CTV.dial CSV,avoiding: :air @19:19:20.84 CSV.dial CTV @19:19:21.18 CS.reconfigure :pressurize @19:19:21.23 CS.seek 0ml @19:19:32.61 CSV.dial :air @19:19:33.07 CS.reconfigure :fast @19:19:33.12 CS.seek 10ml @19:19:34.86 washCycleDA #1 of 3 @19:19:34.87 PSR.select :air @19:19:34.89 PSV.dial PSR @19:19:36.22 Pulling 0.5ml of PSR.wash @19:19:36.23 PSR.select :wash @19:19:36.35 PS.reconfigure :default @19:19:36.40 PS.seek 0.5ml @19:19:42.36 delay 5 seconds @19:19:47.38 PSR.select :air @19:19:47.50 PS.seek 2ml @19:19:57.42 CSV.dial between air and CTV @19:19:57.75 CS.seek 4.274ml @19:20:03.64 PTV.dial :puck @19:20:04.39 PSV.dial PTV @19:20:05.13 PBV.dial :waste1 @19:20:05.56 PS.seek 1.5ml @19:20:11.49 delay 3 seconds @19:20:11.55 CTV.dial CSV,avoiding: :air @19:20:11.58 CSV.dial CTV @19:20:11.95 CS.reconfigure :pressurize @19:20:12.00 CS.seek 0ml @19:20:14.51 PBV.dial between PSV and waste1 @19:20:14.88 PS.seek 0ml @19:20:23.89 CSV.dial :air @19:20:24.41 CS.reconfigure :default @19:20:24.46 CS.hold @19:20:24.59 ShallowSampler::Error in MAIN -- Puck.from 2, :wcr reached only 19.9 of 35psi after pumping in 40ml of gas @19:20:24.61 Waiting to Resume from Checkpoint "Pumping wcr puck from tube 2 up to 35psi..." @19:20:24.62 BadNews.email "Puck.from 2, :wcr reached only 19.9 of 35psi after pumping in 40ml of gas",Subject: "ShallowSampler::Error in MAIN" @19:20:28.59 upsync @19:20:31.06 delay 2:00 @19:22:31.08 recoverPSRreagent08 @19:22:31.10 PTV.dial :puck @19:22:31.28 PBV.dial :waste1 @19:22:31.66 delay 30 seconds @19:23:01.69 PBV.dial between PSV and waste1 @19:23:02.06 PSR.select :air @19:23:02.08 PSV.dial PSR @19:23:02.83 PS.reconfigure :fast @19:23:02.89 PS.seek 2.5ml @19:23:26.76 PSV.dial PTV @19:23:27.64 delay 5 seconds @19:23:32.66 PSV.dial PSR @19:23:33.52 delay 5 seconds @19:23:38.54 PSV.dial PTV @19:23:39.27 delay 5 seconds @19:23:44.29 PSV.dial :air @19:23:45.05 PS.seek 0ml @19:24:08.48 washCycleDA #2 of 3 @19:24:08.50 PSR.select :air @19:24:08.51 PSV.dial PSR @19:24:09.83 Pulling 0.5ml of PSR.wash @19:24:09.84 PSR.select :wash @19:24:09.96 PS.reconfigure :default @19:24:10.01 PS.seek 0.5ml @19:24:16.01 delay 5 seconds @19:24:21.03 PSR.select :air @19:24:21.15 PS.seek 2ml @19:24:37.32 PTV.dial :puck @19:24:37.40 PSV.dial PTV @19:24:38.15 PBV.dial :waste1 @19:24:38.59 PS.seek 1.5ml @19:24:44.51 delay 3 seconds @19:24:47.53 PBV.dial between PSV and waste1 @19:24:47.89 PS.seek 0ml @19:25:04.04 delay 2:00 @19:27:04.07 recoverPSRreagent08 @19:27:04.08 PTV.dial :puck @19:27:04.17 PBV.dial :waste1 @19:27:04.53 delay 30 seconds @19:27:34.55 PBV.dial between PSV and waste1 @19:27:34.91 PSR.select :air @19:27:34.93 PSV.dial PSR @19:27:35.70 PS.reconfigure :fast @19:27:35.75 PS.seek 2.5ml @19:28:00.31 PSV.dial PTV @19:28:01.07 delay 5 seconds @19:28:06.09 PSV.dial PSR @19:28:06.85 delay 5 seconds @19:28:11.87 PSV.dial PTV @19:28:12.65 delay 5 seconds @19:28:17.67 PSV.dial :air @19:28:18.42 PS.seek 0ml @19:28:41.79 washCycleDA #3 of 3 @19:28:41.80 PSR.select :air @19:28:41.82 PSV.dial PSR @19:28:43.15 Pulling 0.5ml of PSR.wash @19:28:43.16 PSR.select :wash @19:28:43.28 PS.reconfigure :default @19:28:43.32 PS.seek 0.5ml @19:28:49.32 delay 5 seconds @19:28:54.34 PSR.select :air @19:28:54.46 PS.seek 2ml @19:29:10.57 PTV.dial :puck @19:29:10.65 PSV.dial PTV @19:29:11.38 PBV.dial :waste1 @19:29:11.80 PS.seek 1.5ml @19:29:17.76 delay 3 seconds @19:29:20.78 PBV.dial between PSV and waste1 @19:29:21.14 PS.seek 0ml @19:29:37.23 delay 2:00 @19:31:37.25 recoverPSRreagent08 @19:31:37.26 PTV.dial :puck @19:31:37.33 PBV.dial :waste1 @19:31:37.71 delay 30 seconds @19:32:07.73 PBV.dial between PSV and waste1 @19:32:08.10 PSR.select :air @19:32:08.12 PSV.dial PSR @19:32:08.88 PS.reconfigure :fast @19:32:08.93 PS.seek 2.5ml @19:32:33.03 PSV.dial PTV @19:32:33.80 delay 5 seconds @19:32:38.82 PSV.dial PSR @19:32:39.58 delay 5 seconds @19:32:44.60 PSV.dial PTV @19:32:45.35 delay 5 seconds @19:32:50.37 PSV.dial :air @19:32:51.13 PS.seek 0ml @19:33:14.53 Gate.power :camera,:ON @19:33:14.55 Mixing DA Substrates @19:33:14.56 PSR.select :air @19:33:14.57 PSV.dial PSR @19:33:15.96 Pulling 0.25ml of PSR.sub1 @19:33:15.98 PSR.select :sub1 @19:33:16.21 PS.reconfigure :default @19:33:16.34 PS.seek 0.25ml @19:33:19.73 delay 5 seconds @19:33:24.75 Pulling 0.25ml of PSR.sub2 @19:33:24.76 PSR.select :sub2 @19:33:24.88 PS.seek 0.5ml @19:33:28.35 delay 5 seconds @19:33:33.37 PSR.select :air @19:33:33.48 PS.seek 2ml @19:33:49.56 PTV.dial :puck @19:33:49.64 PSV.dial PTV @19:33:50.39 PBV.dial :waste1 @19:33:50.83 PS.seek 1.5ml @19:33:57.06 delay 3 seconds @19:34:00.08 PBV.dial between PSV and waste1 @19:34:00.94 PS.seek 0ml @19:34:17.28 delay 10 seconds @19:34:27.31 recoverPSRreagent08 @19:34:27.32 PTV.dial :puck @19:34:27.39 PBV.dial :waste1 @19:34:27.77 delay 30 seconds @19:34:57.79 PBV.dial between PSV and waste1 @19:34:58.17 PSR.select :air @19:34:58.19 PSV.dial PSR @19:34:58.96 PS.reconfigure :fast @19:34:59.01 PS.seek 2.5ml @19:35:23.22 PSV.dial PTV @19:35:23.99 delay 5 seconds @19:35:29.01 PSV.dial PSR @19:35:29.78 delay 5 seconds @19:35:34.80 PSV.dial PTV @19:35:35.58 delay 5 seconds @19:35:40.60 PSV.dial :air @19:35:41.49 PS.seek 0ml @19:36:05.41 Additional air clear @19:36:05.42 PSR.select :air @19:36:05.43 PSV.dial PSR @19:36:06.79 Pulling 2ml of PSR.air @19:36:06.81 PSR.select :air @19:36:06.83 PS.reconfigure :default @19:36:06.87 PS.seek 2ml @19:36:28.18 delay 5 seconds @19:36:33.21 PTV.dial :puck @19:36:33.29 PSV.dial PTV @19:36:34.08 PBV.dial :waste1 @19:36:34.53 PS.seek 1.5ml @19:36:40.47 delay 3 seconds @19:36:43.48 PBV.dial between PSV and waste1 @19:36:43.87 delay 1 second @19:36:44.89 PSV.dial :waste1 @19:36:45.96 PS.seek 0ml @19:37:02.11 Processing Puck Heater at 28C before imaging @19:37:02.12 PH.coast @19:37:02.23 Imaging da puck in Processing Clamp while storing it to tube 1 @19:37:02.41 SC.reconfigure :tight @19:37:02.46 SC.seek 1 @19:37:06.08 SC.configure :limp @19:37:06.29 Elbow.reconfigure :tight @19:37:06.34 Elbow.seek 1 @19:37:08.12 Forearm.reconfigure :tight @19:37:08.17 Forearm.seek 1 + 450 counts @19:37:09.22 SE.reconfigure :pick @19:37:09.28 SE.seek :beamBreak @19:37:11.89 SE.configure :default @19:37:12.48 Elbow.reconfigure :limp @19:37:12.59 Forearm.reconfigure :loose @19:37:12.64 Forearm.seek 1 @19:37:12.87 Elbow.hold @19:37:12.94 Elbow.reconfigure :tight @19:37:13.05 SE.seek 9.08 Pucks below Top Plate @19:37:13.50 Forearm.force -20 @19:37:13.54 Forearm.seek 1 + 311 counts @19:37:13.85 Forearm.reconfigure :tight @19:37:13.90 Forearm.seek 1 + 450 counts @19:37:14.11 SE.seek 10.08 Pucks below Top Plate @19:37:14.76 Forearm.reconfigure :loose @19:37:14.81 Forearm.seek :retract @19:37:15.74 Elbow.seek PC @19:37:18.45 Elbow.reconfigure :loose @19:37:18.55 Forearm.seek PC @19:37:19.52 Hand.close @19:37:20.20 PC.seek :open @19:37:24.98 Forearm.seek :retract @19:37:25.93 Elbow.reconfigure :tight @19:37:25.98 Elbow.seek 1 @19:37:28.53 Forearm.reconfigure :tight @19:37:28.58 Forearm.seek 1 + 450 counts @19:37:29.55 Hand.open @19:37:30.26 SE.seek 9.73 Pucks below Top Plate @19:37:30.50 SE.reconfigure :seal @19:37:30.55 SE.seek 8.77 Pucks below Top Plate @19:37:31.16 Lights, camera, ACTION! @19:37:31.20 PuckCamera: snap -bin=2,2 -auto /var/log/esp/da23sep2619h475ml.tif @19:37:50.61 Waiting >20 seconds for da to relinquish FlushPuck @19:37:57.31 SX HX9/16 (USB:03): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 22.764 seconds /var/log/esp/da23sep2619h475ml.tif: TIFF Upload Complete @19:37:57.34 PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da23sep2619h475ml15s.tif @19:38:12.95 SX HX9/16 (USB:03): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15 seconds /var/log/esp/midres/da23sep2619h475ml15s.tif: TIFF Upload Complete @19:38:12.98 PuckCamera: snap -bin=2,2 20.0 /var/log/esp/midres/da23sep2619h475ml20s.tif @19:38:33.63 SX HX9/16 (USB:03): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 20 seconds /var/log/esp/midres/da23sep2619h475ml20s.tif: TIFF Upload Complete @19:38:33.65 Gate.power :camera,:OFF @19:38:33.67 DAfans.email "22.764 sec auto-exposure",Subject: "da23sep2619h475ml images!" @19:38:34.10 Elbow.seek 1 @19:38:34.87 Forearm.seek 1 + 450 counts @19:38:34.96 SE.reconfigure :gently @19:38:35.02 SE.seek 9.49 Pucks below Top Plate @19:38:35.74 Elbow.reconfigure :limp @19:38:35.80 Forearm.reconfigure :loose @19:38:35.86 Forearm.seek 1 @19:38:36.16 Elbow.hold @19:38:36.23 Elbow.reconfigure :tight @19:38:36.28 SE.reconfigure :default @19:38:36.35 SE.seek 9.67 Pucks below Top Plate @19:38:36.72 Forearm.force -20 @19:38:36.76 Forearm.seek 1 + 313 counts @19:38:37.35 Forearm.reconfigure :tight @19:38:37.48 Forearm.seek 1 + 450 counts @19:38:37.77 SE.seek :down @19:38:39.06 Forearm.reconfigure :loose @19:38:39.17 Forearm.seek :retract @19:38:39.86 SE.stop @19:38:40.29 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @19:38:40.68 Elbow.seek FlushPuck::Garage @19:38:42.67 Elbow.reconfigure :loose @19:38:42.88 Forearm.seek :garage @19:38:43.99 Hand.close @19:38:44.89 Forearm.seek :retract @19:38:45.95 Elbow.reconfigure :tight @19:38:46.04 Elbow.seek PC @19:38:47.50 Elbow.reconfigure :loose @19:38:47.71 Forearm.reconfigure :tight @19:38:48.84 Forearm.seek PC @19:38:50.55 PC.seek :closed @19:38:55.34 Processing Clamp onSpring/offSpring for 16/26 samples, on/off torque 1.61 @19:38:55.48 Hand.open @19:38:56.22 Forearm.reconfigure :loose @19:38:56.30 Forearm.seek :retract @19:38:57.31 PSR.select :air @19:38:57.35 PSV.dial PSR @19:38:57.88 Pulling 0.5ml of PSR.flush @19:38:57.90 PSR.select :flush @19:38:58.07 PS.seek 0.5ml @19:39:04.05 delay 5 seconds @19:39:07.78 upsync @19:39:09.08 PSR.select :air @19:39:09.20 PS.seek 2.5ml @19:39:30.46 delay 1 second @19:39:31.48 PRV.dial :waste2,avoiding: :ab2 @19:39:33.35 PTV.dial :PRVmixing @19:39:34.65 PSV.dial :mixloop @19:39:35.69 PS.seek :empty @19:40:01.99 PSR.select :air @19:40:02.01 PSV.dial PSR @19:40:03.06 Pulling 0.5ml of PSR.flush @19:40:03.07 PSR.select :flush @19:40:03.19 PS.seek 0.5ml @19:40:09.15 delay 5 seconds @19:40:14.17 PSR.select :air @19:40:14.29 PS.seek 2.5ml @19:40:35.49 delay 1 second @19:40:36.51 PBV.dial :waste1 @19:40:36.88 PTV.dial :mixing @19:40:37.63 PSV.dial :mixloop @19:40:38.81 PS.seek :empty @19:41:05.21 PSV.dial PSR @19:41:06.24 PSR.select :flush @19:41:06.36 PS.seek 1ml @19:41:17.55 PSR.select :air @19:41:17.67 PS.seek 2.5ml @19:41:33.80 PSV.dial PTV @19:41:34.55 PTV.dial :puck @19:41:35.75 PBV.dial :waste1 @19:41:35.83 PS.seek 0ml @19:42:02.18 PSV.dial PSR @19:42:02.93 PSR.select :flush @19:42:03.05 PS.seek 0.5ml @19:42:08.96 PSR.select :air @19:42:09.08 PS.seek 2.5ml @19:42:30.36 PSV.dial PTV @19:42:31.12 PBV.dial PSV @19:42:31.68 PS.seek 0ml @19:42:58.02 PSV.dial PBV @19:42:58.50 PS.seek 2.5ml @19:43:24.86 PSV.dial :waste1 @19:43:25.62 PS.seek 0ml @19:43:52.02 PSV.dial PSR @19:43:52.49 PSR.select :air @19:43:52.51 PS.seek 2.5ml @19:44:18.89 PSV.dial PTV @19:44:19.63 PBV.dial PSV @19:44:19.82 PS.seek 0ml @19:44:46.36 PSV.dial PBV @19:44:46.84 PS.seek 2.5ml @19:45:13.23 PSV.dial :waste1 @19:45:13.98 PS.seek 0ml @19:45:40.52 PSV.dial PSR @19:45:40.99 PSR.select :flush @19:45:41.11 PS.seek 1ml @19:45:52.20 PSR.select :air @19:45:52.32 PS.seek 2.5ml @19:46:08.48 PSV.dial PTV @19:46:09.22 PTV.dial :puck @19:46:09.30 PBV.dial :waste1 @19:46:09.85 PS.seek 0ml @19:46:36.45 PSV.dial PSR @19:46:37.20 PSR.select :flush @19:46:37.33 PS.seek 0.5ml @19:46:43.30 PSR.select :air @19:46:43.41 PS.seek 2.5ml @19:47:04.73 PSV.dial PTV @19:47:05.48 PBV.dial PSV @19:47:06.05 PS.seek 0ml @19:47:32.39 PSV.dial PBV @19:47:32.86 PS.seek 2.5ml @19:47:59.67 PSV.dial :waste1 @19:48:00.41 PS.seek 0ml @19:48:26.83 PSV.dial PSR @19:48:27.29 PSR.select :air @19:48:27.32 PS.seek 2.5ml @19:48:53.73 PSV.dial PTV @19:48:54.47 PBV.dial PSV @19:48:54.66 PS.seek 0ml @19:49:21.04 PSV.dial PBV @19:49:21.52 PS.seek 2.5ml @19:49:47.89 PSV.dial :waste1 @19:49:48.63 PS.seek 0ml @19:50:15.04 Garaging FlushPuck in Processing Clamp @19:50:15.12 Elbow.reconfigure :tight @19:50:15.17 Elbow.seek PC @19:50:15.29 Elbow.reconfigure :loose @19:50:15.39 Forearm.seek PC @19:50:16.32 Hand.close @19:50:16.94 PC.seek :open @19:50:21.69 Forearm.seek :retract @19:50:22.64 Elbow.reconfigure :tight @19:50:22.69 Elbow.seek :garage @19:50:24.02 Elbow.reconfigure :loose @19:50:24.12 Forearm.reconfigure :tight @19:50:24.17 Forearm.seek :garage @19:50:25.21 Hand.open @19:50:25.87 Forearm.reconfigure :loose @19:50:25.92 Forearm.seek :retract @23:55:32.98 Session Begun @23:55:39.16 -> CC Collection Clamp contains wcr puck from tube 2 @23:55:41.78 -> PC Processing Clamp open @23:55:48.32 -> Hand Hand is opened @23:55:51.58 -> Arm Elbow at garage, Forearm retracted, Hand is opened @23:56:21.92 -> MainThread.resume @23:56:21.94
Resuming Thread (suspended since 19:17:21) # @23:56:21.99 Pumping wcr puck from tube 2 up to 35psi... @23:56:22.03 CBV.dial SSV @23:56:23.57 CBV.dial between CSV and waste2b @23:56:24.55 CSV.dial between air and CTV @23:56:24.89 CS.reconfigure :fast @23:56:24.97 CS.seek 5.886ml @23:56:40.21 CTV.dial CSV,avoiding: :air @23:56:40.24
CSV.dial CTV @23:56:40.62 CS.reconfigure :pressurize @23:56:40.70 CS.seek 0ml @23:56:56.36 CSV.dial :air @23:56:56.84 CS.reconfigure :fast @23:56:56.91 CS.seek 10ml @23:57:21.46 CSV.dial between air and CTV @23:57:21.79 CS.seek 5.92ml @23:57:31.84 CTV.dial CSV,avoiding: :air @23:57:31.87
CSV.dial CTV @23:57:32.24 CS.reconfigure :pressurize @23:57:32.32 CS.seek 0ml @23:57:48.05 CSV.dial :air @23:57:48.52 CS.reconfigure :fast @23:57:48.59 CS.seek 10ml @23:58:12.92 CSV.dial between air and CTV @23:58:13.26 CS.seek 3.965ml @23:58:27.68 CTV.dial CSV,avoiding: :air @23:58:27.71
CSV.dial CTV @23:58:28.09 CS.reconfigure :pressurize @23:58:28.16 CS.seek 0ml @23:58:38.89 CSV.dial :air @23:58:39.37 CS.reconfigure :fast @23:58:39.44 CS.seek 10ml @23:59:03.88 CSV.dial between air and CTV @23:59:04.27 CS.seek 4.027ml @23:59:19.12 CTV.dial CSV,avoiding: :air @23:59:19.15
CSV.dial CTV @23:59:19.51 CS.reconfigure :pressurize @23:59:19.58 CS.seek 0ml @23:59:30.48 CSV.dial :air @23:59:30.96 CS.reconfigure :default @23:59:31.03 CS.hold @23:59:31.17 ShallowSampler::Error in MAIN -- Puck.from 2, :wcr reached only 30.7 of 35psi after pumping in 40ml of gas @23:59:31.19 Waiting to Resume from Checkpoint "Pumping wcr puck from tube 2 up to 35psi..." @23:59:31.22 BadNews.email "Puck.from 2, :wcr reached only 30.7 of 35psi after pumping in 40ml of gas",Subject: "ShallowSampler::Error in MAIN" @23:59:35.54 upsync @00:01:03.81UTC27-Sep-23 -> CC.unloadPuck 1, :WCR @00:01:03.91 Puck::Warning in dave -- Unloading Puck.from 2, :wcr to Storage Carousel @00:01:03.93 Storing WCR puck in Collection Clamp to tube 1 @00:01:04.15 SC.reconfigure :tight @00:01:04.27 SC.seek 1 @00:01:04.36 SC.configure :limp @00:01:04.66 Elbow.reconfigure :tight @00:01:04.73 Elbow.seek 1 @00:01:06.63 Forearm.reconfigure :tight @00:01:06.70 Forearm.seek 1 + 450 counts @00:01:07.85 SE.reconfigure :pick @00:01:07.93 SE.seek :beamBreak @00:01:10.41 SE.configure :default @00:01:10.59 Elbow.reconfigure :limp @00:01:10.66 Forearm.reconfigure :loose @00:01:10.73 Forearm.seek 1 @00:01:11.05 Elbow.hold @00:01:11.14 Elbow.reconfigure :tight @00:01:11.26 SE.seek 10.09 Pucks below Top Plate @00:01:11.75 Forearm.force -20 @00:01:11.81 Forearm.seek 1 + 315 counts @00:01:12.11 Forearm.reconfigure :tight @00:01:12.18 Forearm.seek 1 + 450 counts @00:01:12.74 SE.seek 11.09 Pucks below Top Plate @00:01:13.34 Forearm.reconfigure :loose @00:01:13.40 Forearm.seek :retract @00:01:14.37 Elbow.seek CC @00:01:16.02 Elbow.reconfigure :loose @00:01:16.14 Forearm.seek CC @00:01:17.16 Hand.close @00:01:17.85 CC.seek :open @00:01:22.65 Forearm.seek :retract @00:01:23.63 Elbow.reconfigure :tight @00:01:23.70 Elbow.seek 1 @00:01:25.49 Elbow.reconfigure :limp @00:01:25.56 Forearm.seek 1 @00:01:26.57 Elbow.hold @00:01:26.65 Elbow.reconfigure :tight @00:01:26.72 Hand.open @00:01:27.47 Forearm.force -20 @00:01:27.53 Forearm.seek 1 + 306 counts @00:01:27.83 Forearm.reconfigure :tight @00:01:27.90 Forearm.seek 1 + 450 counts @00:01:28.18 SE.seek :down @00:01:29.31 Forearm.reconfigure :loose @00:01:29.38 Forearm.seek :retract @00:01:30.17 SE.stop @00:03:07.30 -> MainThread.resume @00:03:07.32
Resuming Thread (suspended since 19:17:21) # @00:03:07.36 Pumping wcr puck from tube 2 up to 35psi... @00:03:07.40 CBV.dial SSV @00:03:08.83 CBV.dial between CSV and waste2b @00:03:09.79 CSV.dial between air and CTV @00:03:10.13 CS.reconfigure :fast @00:03:10.20 CS.seek 7.314ml @00:03:28.77 CTV.dial CSV,avoiding: :air @00:03:28.80
CSV.dial CTV @00:03:29.17 CS.reconfigure :pressurize @00:03:29.70 CS.seek 0ml @00:03:48.98 CSV.dial :air @00:03:49.46 CS.reconfigure :fast @00:03:49.53 CS.seek 10ml @00:04:13.66 CSV.dial between air and CTV @00:04:14.01 CS.seek 7.349ml @00:04:20.83 CTV.dial CSV,avoiding: :air @00:04:20.86
CSV.dial CTV @00:04:21.20 CS.reconfigure :pressurize @00:04:21.27 CS.seek 0ml @00:04:40.67 CSV.dial :air @00:04:41.14 CS.reconfigure :fast @00:04:41.22 CS.seek 10ml @00:05:05.25 CSV.dial between air and CTV @00:05:05.58 CS.seek 7.332ml @00:05:12.36 CTV.dial CSV,avoiding: :air @00:05:12.39
CSV.dial CTV @00:05:12.77 CS.reconfigure :pressurize @00:05:12.84 CS.seek 0ml @00:05:32.20 CSV.dial :air @00:05:32.67 CS.reconfigure :fast @00:05:32.74 CS.seek 10ml @00:05:56.66 CSV.dial between air and CTV @00:05:57.00 CS.seek 7.332ml @00:06:03.76 CTV.dial CSV,avoiding: :air @00:06:03.79
CSV.dial CTV @00:06:04.17 CS.reconfigure :pressurize @00:06:04.24 CS.seek 0ml @00:06:23.60 CSV.dial :air @00:06:24.07 CS.reconfigure :default @00:06:24.14 CS.hold @00:06:24.29 ShallowSampler::Error in MAIN -- reached only -0.2 of 35psi after pumping in 40ml of gas @00:06:24.69 Waiting to Resume from Checkpoint "Pumping wcr puck from tube 2 up to 35psi..." @00:06:24.77 BadNews.email " reached only -0.2 of 35psi after pumping in 40ml of gas",Subject: "ShallowSampler::Error in MAIN" @00:06:28.84 upsync @00:06:33.03 -> CC Collection Clamp open @00:06:35.60 -> PC Processing Clamp open @00:06:42.16 -> Puck.count @00:06:42.25 SE.seek :down @00:06:42.34 SE.stop @00:06:42.46 SC.reconfigure :tight @00:06:42.54 SC.seek 1 @00:06:42.63 SC.configure :limp @00:06:42.81 Elbow.seek 1 @00:06:43.03 Forearm.reconfigure :tight @00:06:43.10 Forearm.seek 1 + 450 counts @00:06:44.14 SE.reconfigure :place @00:06:44.23 SE.seek :beamBreak @00:06:46.94 SE.configure :default @00:06:47.07 SE.seek :down @00:06:48.21 Forearm.reconfigure :loose @00:06:48.28 Forearm.seek :retract @00:06:48.99 SE.stop @00:06:49.30 SC.reconfigure :tight @00:06:49.37 SC.seek 2 @00:06:52.96 SC.configure :limp @00:06:53.15 Elbow.seek 2 @00:06:53.36 Forearm.reconfigure :tight @00:06:53.45 Forearm.seek 2 + 450 counts @00:06:54.50 SE.reconfigure :place @00:06:54.58 SE.seek :beamBreak @00:06:56.64 SE.configure :default @00:06:56.77 SE.seek :down @00:06:57.91 Forearm.reconfigure :loose @00:06:57.98 Forearm.seek :retract @00:06:58.14 SE.stop @00:06:58.99 SC.reconfigure :tight @00:06:59.06 SC.seek 3 @00:07:02.57 SC.configure :limp @00:07:02.76 Elbow.seek 3 @00:07:03.31 Forearm.reconfigure :tight @00:07:03.38 Forearm.seek 3 + 450 counts @00:07:04.44 SE.reconfigure :place @00:07:04.52 SE.seek :beamBreak @00:07:05.68 SE.configure :default @00:07:05.81 SE.seek :down @00:07:06.23 SE.stop @00:07:06.94 Forearm.reconfigure :loose @00:07:07.01 Forearm.seek :retract @00:07:07.96 SC.reconfigure :tight @00:07:08.08 SC.seek 4 @00:07:11.70 SC.configure :limp @00:07:11.87 Elbow.seek 4 @00:07:12.39 Forearm.reconfigure :tight @00:07:12.46 Forearm.seek 4 + 450 counts @00:07:13.56 SE.reconfigure :place @00:07:13.64 SE.seek :beamBreak @00:07:14.79 SE.configure :default @00:07:14.92 SE.seek :down @00:07:15.37 SE.stop @00:07:16.05 Forearm.reconfigure :loose @00:07:16.12 Forearm.seek :retract @00:07:17.08 SC.reconfigure :tight @00:07:17.15 SC.seek 5 @00:07:20.68 SC.configure :limp @00:07:20.86 Elbow.seek 5 @00:07:21.13 Forearm.reconfigure :tight @00:07:21.21 Forearm.seek 5 + 450 counts @00:07:22.30 SE.reconfigure :place @00:07:22.38 SE.seek :beamBreak @00:07:23.54 SE.configure :default @00:07:23.67 SE.seek :down @00:07:24.58 SE.stop @00:07:24.80 Forearm.reconfigure :loose @00:07:24.88 Forearm.seek :retract @00:07:25.90 SC.reconfigure :tight @00:07:25.97 SC.seek 6 @00:07:29.60 SC.configure :limp @00:07:29.78 Elbow.seek 6 @00:07:30.00 Forearm.reconfigure :tight @00:07:30.07 Forearm.seek 6 + 450 counts @00:07:31.15 SE.reconfigure :place @00:07:31.24 SE.seek :beamBreak @00:07:32.42 SE.configure :default @00:07:32.55 SE.seek :down @00:07:32.98 SE.stop @00:07:33.68 Forearm.reconfigure :loose @00:07:33.75 Forearm.seek :retract @00:07:34.73 SC.reconfigure :tight @00:07:34.80 SC.seek 7 @00:07:38.34 SC.configure :limp @00:07:38.51 Elbow.seek 7 @00:07:39.09 Forearm.reconfigure :tight @00:07:39.16 Forearm.seek 7 + 450 counts @00:07:40.22 SE.reconfigure :place @00:07:40.30 SE.seek :beamBreak @00:07:41.64 SE.configure :default @00:07:41.77 SE.seek :down @00:07:42.37 SE.stop @00:07:42.90 Forearm.reconfigure :loose @00:07:42.98 Forearm.seek :retract {1 => 12, 2 => 21, 3 => 33, 4 => 33, 5 => 33, 6 => 33, 7 => 31} @00:08:11.59 -> purgeIntake @00:08:11.61 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @00:08:11.82 Elbow.seek FlushPuck::Garage @00:08:13.68 Elbow.reconfigure :loose @00:08:13.80 Forearm.seek :garage @00:08:14.78 Hand.close @00:08:16.04 Forearm.seek :retract @00:08:17.06 Elbow.reconfigure :tight @00:08:17.14 Elbow.seek CC @00:08:20.09 Elbow.reconfigure :loose @00:08:20.21 Forearm.reconfigure :tight @00:08:20.28 Forearm.seek CC @00:08:21.60 CC.seek :closed @00:08:26.20 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.41 @00:08:26.29 Hand.open @00:08:27.03 Forearm.reconfigure :loose @00:08:27.10 Forearm.seek :retract @00:08:28.13 Purging external Intake @00:08:28.27 CSV.dial :flush @00:08:28.73 CS.reconfigure :fast @00:08:28.80 CS.seek 10ml @00:08:53.32 delay 5 seconds @00:08:58.34 CSV.dial CBV @00:08:58.38 CBV.dial CSV @00:08:59.16 CTV.dial :intake @00:08:59.94 Intake.select :open @00:09:00.07 CS.seek 0ml @00:09:23.47 Intake.select :close @00:09:23.60 CTV.dial :air @00:09:24.37 Garaging FlushPuck in Collection Clamp @00:09:24.46 Elbow.reconfigure :tight @00:09:24.53 Elbow.seek CC @00:09:24.80 Elbow.reconfigure :loose @00:09:24.93 Forearm.seek CC @00:09:26.00 Hand.close @00:09:26.59 CC.seek :open @00:09:31.24 Forearm.seek :retract @00:09:32.30 Elbow.reconfigure :tight @00:09:32.37 Elbow.seek :garage @00:09:35.30 Elbow.reconfigure :loose @00:09:35.43 Forearm.reconfigure :tight @00:09:35.90 Forearm.seek :garage @00:09:36.96 Hand.open @00:09:37.66 Forearm.reconfigure :loose @00:09:37.72 Forearm.seek :retract FlushPuck::Garage @00:12:48.65 -> CS.flush @00:12:48.67 NoMethodError in dave -- undefined method `flush' for CS:Syringe @00:14:37.05 -> abort 'wcr failed' @00:14:47.93 -> abort "wcr failed" @00:15:00.53 -> abort! "wcr failed" @00:15:00.54 CmdServer::Client::Error in dave -- Aborting Mission: wcr failed @00:15:00.59 BadNews.email "wcr failed",Subject: "DA_WCR_92623 ABORTED!" # @00:15:04.62 upsync @00:15:04.72 Session Ended + ESPwhich DA_WCR_92823.rb + fn=DA_WCR_92823.rb + IFS=: + set /home/esp/esp2/mission . + fullPath=/home/esp/esp2/mission/DA_WCR_92823.rb + [ -f /home/esp/esp2/mission/DA_WCR_92823.rb ] + realpath /home/esp/esp2/mission/DA_WCR_92823.rb + return 0 + scriptPath=/home/esp/esp2local/mission/DA_WCR_92823.rb + exec ruby /home/esp/esp2/lib/esp.rb /home/esp/esp2local/mission/DA_WCR_92823.rb @13:56:14.80UTC28-Sep-23
ESP2 v3.42 I2C Gateway v3.20 for core bus @13:56:17.44 Configuration for short puck jake 1/11/21 by brent@mbari.org /home/esp/esp2/type/shallow/jake/configure.rb @13:56:21.09 Polling Can every 10:00 @13:56:21.16 Cleanly exited at 13:52:07UTC28-Sep-23 @13:56:21.20 Loading /home/esp/esp2local/mission/DA_WCR_92823.rb mission @13:56:21.26 GoodNews.email "Starting DA_WCR_92823 w/pucks in tube 2\n{1 => 12,\n 2 => 21,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@13:56:21, 27% flow, 18.0C, 12% humidity, 14.6psia, 13.759V, 0.254A, 56.956Ah, 3.50W",Subject: "DA_WCR_92823 begun" @13:56:21.30
Preparing to sleep until + 00:00:05 @13:56:21.34 Asleep til + 00:00:05 @13:56:21.35 Continuing at 13:56 @13:56:21.64 Gate.power :core,:OFF @13:56:21.65 Gate.power :raw,:OFF @13:56:21.66 Shutdown @13:56:24.29 GoodNews.email "Then, in tube 2: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_92823.rb:14\n{1 => 12,\n 2 => 21,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@13:56:21, 26% flow, 18.0C, 12% humidity, 14.6psia, 13.759V, 0.260A, 56.956Ah, 3.58W",Subject: "Asleep until 13:56" @13:56:25.63 upsync @13:56:26.25
Powering core bus @13:56:28.12 upsync @13:56:31.62
Gate.power :raw,:ON @13:56:32.28 CS.configure :default @13:56:32.33 PS.configure :default @13:56:32.37 SS.configure :default @13:56:32.42 CC.configure :default @13:56:32.47 PC.configure :default @13:56:32.54 Forearm.configure :loose @13:56:32.59 Elbow.configure :loose @13:56:32.66 SC.configure :loose @13:56:32.71 SE.configure :default @13:56:32.76 CH.configure :default @13:56:32.79 PH.configure :default @13:56:32.83 All dwarf controllers running firmware version 3.85 @13:56:32.94 Valve.reset @13:56:33.28 PSV.dial :air @13:56:33.30 Intake.select :close @13:56:33.35 Exhaust.select :close @13:56:33.39 CTV.dial :air @13:56:33.47
Forearm.home.seek :retract @13:56:33.53 CSV.dial :waste1 @13:56:33.98 SSV.dial :spit,avoiding: :exhaust @13:56:34.24 Hand.close @13:56:34.28
Elbow.reconfigure :loose @13:56:34.40 Elbow.jog 1500 @13:56:34.42 PS.home.seek 0ml @13:56:34.85 CS.reconfigure :fast @13:56:34.89 SS.home.seek 0ml @13:56:34.98 CS.home.seek 0ml @13:56:35.06
Elbow.reconfigure :loose @13:56:35.11 Elbow.home.seek :clear @13:56:39.60 SE.home.seek :down @13:56:40.22 SC.reconfigure :tight @13:56:40.27 SC.home.seek :initial @13:56:42.74 PS.stop @13:57:08.58
SC.configure :limp @13:57:08.64 Forearm.seek :retract @13:57:08.70 Elbow.seek :hammer @13:57:09.75 Forearm.reconfigure :tight @13:57:09.80 Forearm.seek retracted @13:57:10.52 PC.home.seek :open @13:57:10.61
CC.home.seek :open @13:57:14.00 Phase [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] @13:57:14.01 Notice.email "At 13:57:13 began in tube 2: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_92823.rb:14\n{1 => 12,\n 2 => 21,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@13:57:13, 27% flow, 18.0C, 13% humidity, 14.6psia, 13.759V, 0.272A, 56.961Ah, 3.75W",Subject: "Started DA_WCR_92823 phase initialPurge; da [1000, 1000] #da and wsr are run simultanelously" @13:57:14.04
Starting initialPurge @13:57:14.06 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @13:57:14.12 Hand.open @13:57:14.83 Elbow.reconfigure :tight @13:57:14.88 Elbow.seek FlushPuck::Garage @13:57:16.80 Elbow.reconfigure :loose @13:57:17.05 Forearm.reconfigure :loose @13:57:17.10 Forearm.seek :garage @13:57:18.05 Hand.close @13:57:18.10 upsync @13:57:18.82
Forearm.seek :retract @13:57:19.83 Elbow.reconfigure :tight @13:57:19.88 Elbow.seek CC @13:57:22.79 Elbow.reconfigure :loose @13:57:22.90 Forearm.reconfigure :tight @13:57:22.95 Forearm.seek CC @13:57:24.22 CC.seek :closed @13:57:28.84 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.36 @13:57:28.90 Hand.open @13:57:29.62 Forearm.reconfigure :loose @13:57:29.67 Forearm.seek :retract @13:57:30.66 Flushing the Collection Valve::Manifold @13:57:30.78 CSV.dial CSR @13:57:32.06 Pulling 5ml of CSR.flush @13:57:32.07 CSR.select :flush @13:57:32.18 CS.reconfigure :default @13:57:32.64 CS.seek 5ml @13:57:46.36 delay 5 seconds @13:57:51.37 CSR.select :air @13:57:51.55 CS.reconfigure :fast @13:57:51.59 CS.seek 7.5ml @13:57:58.32 CSV.dial :waste @13:57:59.61 CS.reconfigure :default @13:57:59.66 CS.seek 0ml @13:58:19.67 Purging external Intake @13:58:19.79 CSV.dial :flush @13:58:20.36 CS.reconfigure :fast @13:58:20.41 CS.seek 10ml @13:58:44.76 delay 5 seconds @13:58:49.78 CSV.dial CBV @13:58:49.83
CBV.dial CSV @13:58:50.53 CTV.dial :intake @13:58:51.32 Intake.select :open @13:58:51.44 CS.seek 0ml @13:59:14.85 Intake.select :close @13:59:14.95 CTV.dial :air @13:59:15.75 Flushing upper manifold @13:59:15.87 CSV.dial :flush @13:59:16.61 CS.seek 5ml @13:59:29.45 delay 5 seconds @13:59:34.47 CSV.dial :air @13:59:34.98 CS.seek 7ml @13:59:40.48 CSV.dial CBV @13:59:40.50
CBV.dial CSV @13:59:41.53 CTV.dial :air @13:59:41.61 CS.seek 0ml @13:59:58.26 delay 7 seconds @14:00:05.29 CS.seek 3ml @14:00:13.29 CSV.dial :waste @14:00:13.75 CS.seek 0ml @14:00:21.56 CSV.dial :flush @14:00:22.00 CS.seek 3ml @14:00:30.01 delay 5 seconds @14:00:35.03 CSV.dial :air @14:00:35.65 CS.seek 5ml @14:00:41.13 CSV.dial :air @14:00:41.22 CS.seek 0ml @14:00:53.41 delay 7 seconds @14:01:00.44 CS.seek 3ml @14:01:08.38 CSV.dial :waste @14:01:09.11 CS.seek 0ml @14:01:16.70 Garaging FlushPuck in Collection Clamp @14:01:16.77 Elbow.reconfigure :tight @14:01:16.81 Elbow.seek CC @14:01:17.08 Elbow.reconfigure :loose @14:01:17.18 Forearm.seek CC @14:01:18.21 Hand.close @14:01:18.81 CC.seek :open @14:01:23.87 Forearm.seek :retract @14:01:24.83 Elbow.reconfigure :tight @14:01:24.87 Elbow.seek :garage @14:01:27.82 Elbow.reconfigure :loose @14:01:27.92 Forearm.reconfigure :tight @14:01:27.97 Forearm.seek :garage @14:01:28.92 Hand.open @14:01:29.62 Forearm.reconfigure :loose @14:01:29.67 Forearm.seek :retract @14:01:30.64 da sampling at most 1000ml, wcr at most 1000ml @14:01:30.67 Loading sh1 puck from tube 2 into Collection Clamp @14:01:30.69 DAfans.email "Started da in tube 2 at 14:01:30UTC28-Sep-23\n{1 => 12,\n 2 => 21,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@13:57:13, 27% flow, 18.0C, 13% humidity, 14.6psia, 13.759V, 0.272A, 56.961Ah, 3.75W",Subject: "da sampling at most 1000ml, wcr at most 1000ml" @14:01:30.97
SC.reconfigure :tight @14:01:31.03 SC.seek 2 @14:01:34.74 upsync @14:01:37.80
SC.configure :limp @14:01:38.04 Elbow.reconfigure :tight @14:01:38.09 Elbow.seek 2 @14:01:40.06 Forearm.reconfigure :tight @14:01:40.12 Forearm.seek 2 + 450 counts @14:01:41.19 SE.reconfigure :pick @14:01:41.25 SE.seek :beamBreak @14:01:42.98 SE.configure :default @14:01:43.15 Elbow.seek 2 @14:01:43.34 Forearm.seek 2 + 300 counts @14:01:43.54 Forearm.reconfigure :loose @14:01:43.59 Hand.close @14:01:44.20 SE.seek :down @14:01:44.42
Forearm.seek :retract @14:01:45.63 SE.stop @14:01:45.84
Elbow.seek CC @14:01:47.44 Elbow.reconfigure :loose @14:01:47.55 Forearm.reconfigure :tight @14:01:47.60 Forearm.seek CC @14:01:48.90 CC.seek :closed @14:01:53.42 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.28 @14:01:53.44 Hand.open @14:01:54.12 Forearm.reconfigure :loose @14:01:54.16 Forearm.seek :retract @14:01:55.20 collectSample 1000ml w/bubble point of 24psi @14:01:55.21 Priming 7ml @14:01:55.22 Intake.select :close @14:01:55.23 Exhaust.select :close @14:01:55.25 SS.hold @14:01:55.47 Calibrating pressure and temperature sensors @14:01:55.49 CTV.dial :air @14:01:55.57 SSV.dial :spit,avoiding: :exhaust @14:01:55.76 SS.reconfigure :fast @14:01:55.81 SS.seek 5ml @14:01:58.82 SSV.dial CBV @14:01:58.84
CBV.dial SSV @14:02:00.19 delay 3.8 seconds @14:02:04.12 Sensor limits: Intake max 117.2psia; Outlet max 109.6psia @14:02:04.13 SSV.dial :spit @14:02:05.46 SS.seek 0ml @14:02:08.03 SSV.dial CBV @14:02:09.34 CTV.dial :intake @14:02:10.13 SSV.dial CBV @14:02:10.16
CBV.dial SSV @14:02:10.31 Allowing 40s for 7.0ml of remaining 7.0ml @14:02:10.32 Intake.select :open @14:02:12.49 Intake @14.8psia, vacuum from 9.3 to 12.3psi @14:02:12.50 SS.reconfigure :inhaling 2.20ml/s @14:02:12.55 SS.seek 7ml @14:02:19.00 Stroke completed -- waiting for pressure to equalize ... @14:02:19.01 SS.reconfigure :exhale @14:02:19.05 SS.hold @14:02:24.33 Intake.select :close @14:02:24.49 SSV.dial :exhaust @14:02:25.23 Exhaust.select :open @14:02:25.35 SS.seek 0ml @14:02:29.22 Exhaust.select :close @14:02:29.34 Sampling 1000.0ml @14:02:29.35 Intake.select :close @14:02:29.36 Exhaust.select :close @14:02:29.37 SS.reconfigure :default @14:02:29.42 SS.hold @14:02:29.62 CTV.dial :intake @14:02:29.85 SSV.dial CBV @14:02:29.88
CBV.dial SSV @14:02:30.67 Allowing 130s for 25.0ml of remaining 1000.0ml @14:02:30.68 Intake.select :open @14:02:32.85 Intake @15.3psia, vacuum from 9.8 to 12.8psi @14:02:32.87 SS.reconfigure :inhaling 2.20ml/s @14:02:32.91 SS.seek 25ml @14:02:43.38 Vacuum too high -- waiting up to 40 seconds @14:02:43.39 SS.reconfigure :exhale @14:02:43.45 SS.hold @14:02:47.35 Intake @13.8psia, vacuum from 8.3 to 11.3psi @14:02:47.37 SS.reconfigure :inhaling 1.76ml/s @14:02:47.41 SS.seek 25ml @14:02:53.20 Stroke completed -- waiting for pressure to equalize ... @14:02:53.21 SS.reconfigure :exhale @14:02:53.26 SS.hold @14:03:01.21 Intake.select :close @14:03:01.36 SSV.dial :exhaust @14:03:02.12 Exhaust.select :open @14:03:02.24 SS.seek 0ml @14:03:11.22 Exhaust.select :close @14:03:11.33 SSV.dial CBV @14:03:11.36
CBV.dial SSV @14:03:12.12 Allowing 130s for 25.0ml of remaining 975.0ml @14:03:12.14 Intake.select :open @14:03:14.30 Intake @15.5psia, vacuum from 10.0 to 13.0psi @14:03:14.32 SS.reconfigure :inhaling 1.76ml/s @14:03:14.37 SS.seek 25ml @14:03:30.04 Vacuum too high -- waiting up to 40 seconds @14:03:30.05 SS.reconfigure :exhale @14:03:30.10 SS.hold @14:03:36.06 Intake @14.8psia, vacuum from 9.3 to 12.3psi @14:03:36.08 SS.reconfigure :inhaling 1.41ml/s @14:03:36.12 SS.seek 25ml @14:03:38.06 Stroke completed -- waiting for pressure to equalize ... @14:03:38.07 SS.reconfigure :exhale @14:03:38.12 SS.hold @14:03:43.25 Intake.select :close @14:03:43.40 SSV.dial :exhaust @14:03:44.17 Exhaust.select :open @14:03:44.28 SS.seek 0ml @14:03:53.19 Exhaust.select :close @14:03:53.30 SSV.dial CBV @14:03:53.32
CBV.dial SSV @14:03:54.11 Allowing 130s for 25.0ml of remaining 950.0ml @14:03:54.12 Intake.select :open @14:03:56.28 Intake @15.7psia, vacuum from 10.0 to 13.0psi @14:03:56.30 SS.reconfigure :inhaling 1.41ml/s @14:03:56.35 SS.seek 25ml @14:04:16.26 Stroke completed -- waiting for pressure to equalize ... @14:04:16.27 SS.reconfigure :exhale @14:04:16.32 SS.hold @14:04:26.01 Intake.select :close @14:04:26.16 SSV.dial :exhaust @14:04:26.91 Exhaust.select :open @14:04:27.02 SS.seek 0ml @14:04:36.04 Exhaust.select :close @14:04:36.16 SSV.dial CBV @14:04:36.18
CBV.dial SSV @14:04:36.95 Allowing 130s for 25.0ml of remaining 925.0ml @14:04:36.96 Intake.select :open @14:04:39.12 Intake @15.6psia, vacuum from 10.0 to 13.0psi @14:04:39.14 SS.reconfigure :inhaling 1.41ml/s @14:04:39.19 SS.seek 25ml @14:04:59.17 Stroke completed -- waiting for pressure to equalize ... @14:04:59.18 SS.reconfigure :exhale @14:04:59.22 SS.hold @14:05:09.01 Intake.select :close @14:05:09.17 SSV.dial :exhaust @14:05:09.93 Exhaust.select :open @14:05:10.04 SS.seek 0ml @14:05:18.76 Exhaust.select :close @14:05:18.87 SSV.dial CBV @14:05:18.89
CBV.dial SSV @14:05:19.65 Allowing 130s for 25.0ml of remaining 900.0ml @14:05:19.66 Intake.select :open @14:05:21.83 Intake @15.7psia, vacuum from 10.0 to 13.0psi @14:05:21.85 SS.reconfigure :inhaling 1.41ml/s @14:05:21.90 SS.seek 25ml @14:05:41.69 Vacuum too high -- waiting up to 40 seconds @14:05:41.70 SS.reconfigure :exhale @14:05:41.76 SS.hold @14:05:45.90 Intake @14.0psia, vacuum from 8.5 to 11.5psi @14:05:45.92 SS.reconfigure :inhaling 1.13ml/s @14:05:45.97 SS.seek 25ml @14:05:47.73 Stroke completed -- waiting for pressure to equalize ... @14:05:47.74 SS.reconfigure :exhale @14:05:47.78 SS.hold @14:05:53.41 Intake.select :close @14:05:53.57 SSV.dial :exhaust @14:05:54.32 Exhaust.select :open @14:05:54.44 SS.seek 0ml @14:06:03.37 Exhaust.select :close @14:06:03.48 SSV.dial CBV @14:06:03.51
CBV.dial SSV @14:06:04.28 Allowing 130s for 25.0ml of remaining 875.0ml @14:06:04.29 Intake.select :open @14:06:06.45 Intake @15.6psia, vacuum from 10.0 to 13.0psi @14:06:06.47 SS.reconfigure :inhaling 1.13ml/s @14:06:06.52 SS.seek 25ml @14:06:30.71 Stroke completed -- waiting for pressure to equalize ... @14:06:30.72 SS.reconfigure :exhale @14:06:30.77 SS.hold @14:06:40.71 Intake.select :close @14:06:40.87 SSV.dial :exhaust @14:06:41.62 Exhaust.select :open @14:06:41.74 SS.seek 0ml @14:06:50.48 Exhaust.select :close @14:06:50.59 SSV.dial CBV @14:06:50.63
CBV.dial SSV @14:06:51.37 Allowing 130s for 25.0ml of remaining 850.0ml @14:06:51.38 Intake.select :open @14:06:53.54 Intake @15.5psia, vacuum from 10.0 to 13.0psi @14:06:53.56 SS.reconfigure :inhaling 1.13ml/s @14:06:53.61 SS.seek 25ml @14:07:18.41 Stroke completed -- waiting for pressure to equalize ... @14:07:18.42 SS.reconfigure :exhale @14:07:18.47 SS.hold @14:07:29.53 Intake.select :close @14:07:29.69 SSV.dial :exhaust @14:07:30.43 Exhaust.select :open @14:07:30.54 SS.seek 0ml @14:07:39.29 Exhaust.select :close @14:07:39.40 SSV.dial CBV @14:07:39.44
CBV.dial SSV @14:07:40.17 Allowing 130s for 25.0ml of remaining 825.0ml @14:07:40.18 Intake.select :open @14:07:42.34 Intake @15.4psia, vacuum from 9.9 to 12.9psi @14:07:42.36 SS.reconfigure :inhaling 1.13ml/s @14:07:42.41 SS.seek 25ml @14:08:07.18 Stroke completed -- waiting for pressure to equalize ... @14:08:07.19 SS.reconfigure :exhale @14:08:07.24 SS.hold @14:08:18.38 Intake.select :close @14:08:18.54 SSV.dial :exhaust @14:08:19.29 Exhaust.select :open @14:08:19.40 SS.seek 0ml @14:08:28.13 Exhaust.select :close @14:08:28.67 SSV.dial CBV @14:08:28.71
CBV.dial SSV @14:08:29.45 Allowing 130s for 25.0ml of remaining 800.0ml @14:08:29.46 Intake.select :open @14:08:31.63 Intake @15.4psia, vacuum from 9.9 to 12.9psi @14:08:31.64 SS.reconfigure :inhaling 1.13ml/s @14:08:31.69 SS.seek 25ml @14:08:56.41 Stroke completed -- waiting for pressure to equalize ... @14:08:56.42 SS.reconfigure :exhale @14:08:56.47 SS.hold @14:09:09.05 Intake.select :close @14:09:09.21 SSV.dial :exhaust @14:09:09.96 Exhaust.select :open @14:09:10.07 SS.seek 0ml @14:09:18.80 Exhaust.select :close @14:09:18.91 SSV.dial CBV @14:09:18.94
CBV.dial SSV @14:09:19.69 Allowing 130s for 25.0ml of remaining 775.0ml @14:09:19.71 Intake.select :open @14:09:21.88 Intake @15.4psia, vacuum from 9.9 to 12.9psi @14:09:21.90 SS.reconfigure :inhaling 1.13ml/s @14:09:21.95 SS.seek 25ml @14:09:44.00 Vacuum too high -- waiting up to 40 seconds @14:09:44.01 SS.reconfigure :exhale @14:09:44.06 SS.hold @14:09:50.08 Intake @14.3psia, vacuum from 8.8 to 11.8psi @14:09:50.09 SS.reconfigure :inhaling 0.91ml/s @14:09:50.14 SS.seek 25ml @14:09:54.48 Stroke completed -- waiting for pressure to equalize ... @14:09:54.49 SS.reconfigure :exhale @14:09:54.54 SS.hold @14:10:03.11 Intake.select :close @14:10:03.27 SSV.dial :exhaust @14:10:04.02 Exhaust.select :open @14:10:04.13 SS.seek 0ml @14:10:13.24 Exhaust.select :close @14:10:13.35 SSV.dial CBV @14:10:13.37
CBV.dial SSV @14:10:14.15 Allowing 130s for 25.0ml of remaining 750.0ml @14:10:14.16 Intake.select :open @14:10:16.32 Intake @15.4psia, vacuum from 9.9 to 12.9psi @14:10:16.34 SS.reconfigure :inhaling 0.91ml/s @14:10:16.39 SS.seek 25ml @14:10:45.78 Stroke completed -- waiting for pressure to equalize ... @14:10:45.86 SS.reconfigure :exhale @14:10:45.91 SS.hold @14:11:00.31 Intake.select :close @14:11:00.46 SSV.dial :exhaust @14:11:01.21 Exhaust.select :open @14:11:01.32 SS.seek 0ml @14:11:10.33 Exhaust.select :close @14:11:10.44 SSV.dial CBV @14:11:10.46
CBV.dial SSV @14:11:11.22 Allowing 130s for 25.0ml of remaining 725.0ml @14:11:11.23 Intake.select :open @14:11:13.40 Intake @15.5psia, vacuum from 10.0 to 13.0psi @14:11:13.42 SS.reconfigure :inhaling 0.91ml/s @14:11:13.47 SS.seek 25ml @14:11:41.00 Vacuum too high -- waiting up to 40 seconds @14:11:41.01 SS.reconfigure :exhale @14:11:41.06 SS.hold @14:11:49.85 Intake @15.3psia, vacuum from 9.8 to 12.8psi @14:11:49.87 SS.reconfigure :inhaling 0.73ml/s @14:11:49.92 SS.seek 25ml @14:11:52.20 Stroke completed -- waiting for pressure to equalize ... @14:11:52.22 SS.reconfigure :exhale @14:11:52.26 SS.hold @14:12:00.45 Intake.select :close @14:12:00.61 SSV.dial :exhaust @14:12:01.35 Exhaust.select :open @14:12:01.46 SS.seek 0ml @14:12:10.19 Exhaust.select :close @14:12:10.30 SSV.dial CBV @14:12:10.32
CBV.dial SSV @14:12:11.10 Allowing 130s for 25.0ml of remaining 700.0ml @14:12:11.11 Intake.select :open @14:12:13.27 Intake @15.5psia, vacuum from 10.0 to 13.0psi @14:12:13.29 SS.reconfigure :inhaling 0.73ml/s @14:12:13.34 SS.seek 25ml @14:12:49.36 Stroke completed -- waiting for pressure to equalize ... @14:12:49.37 SS.reconfigure :exhale @14:12:49.77 SS.hold @14:13:06.18 Intake.select :close @14:13:06.34 SSV.dial :exhaust @14:13:07.08 Exhaust.select :open @14:13:07.19 SS.seek 0ml @14:13:15.88 Exhaust.select :close @14:13:15.99 SSV.dial CBV @14:13:16.02
CBV.dial SSV @14:13:16.78 Allowing 130s for 25.0ml of remaining 675.0ml @14:13:16.79 Intake.select :open @14:13:18.97 Intake @15.4psia, vacuum from 9.9 to 12.9psi @14:13:18.99 SS.reconfigure :inhaling 0.73ml/s @14:13:19.04 SS.seek 25ml @14:13:54.93 Vacuum too high -- waiting up to 40 seconds @14:13:54.94 SS.reconfigure :exhale @14:13:54.99 SS.hold @14:14:06.46 Intake @16.1psia, vacuum from 10.0 to 13.0psi @14:14:06.48 SS.reconfigure :inhaling 0.59ml/s @14:14:06.53 SS.seek 25ml @14:14:07.33 Stroke completed -- waiting for pressure to equalize ... @14:14:07.34 SS.reconfigure :exhale @14:14:07.38 SS.hold @14:14:17.16 Intake.select :close @14:14:17.31 SSV.dial :exhaust @14:14:18.06 Exhaust.select :open @14:14:18.18 SS.seek 0ml @14:14:27.24 Exhaust.select :close @14:14:27.36 SSV.dial CBV @14:14:27.39
CBV.dial SSV @14:14:28.14 Allowing 130s for 25.0ml of remaining 650.0ml @14:14:28.15 Intake.select :open @14:14:30.32 Intake @15.5psia, vacuum from 10.0 to 13.0psi @14:14:30.33 SS.reconfigure :inhaling 0.59ml/s @14:14:30.38 SS.seek 25ml @14:15:14.72 Stroke completed -- waiting for pressure to equalize ... @14:15:14.73 SS.reconfigure :exhale @14:15:14.77 SS.hold @14:15:33.13 Intake.select :close @14:15:33.29 SSV.dial :exhaust @14:15:34.03 Exhaust.select :open @14:15:34.14 SS.seek 0ml @14:15:42.92 Exhaust.select :close @14:15:43.03 SSV.dial CBV @14:15:43.06
CBV.dial SSV @14:15:43.83 Allowing 130s for 25.0ml of remaining 625.0ml @14:15:43.84 Intake.select :open @14:15:46.00 Intake @15.3psia, vacuum from 9.8 to 12.8psi @14:15:46.02 SS.reconfigure :inhaling 0.59ml/s @14:15:46.07 SS.seek 25ml @14:16:30.93 Vacuum too high -- waiting up to 40 seconds @14:16:30.94 SS.reconfigure :exhale @14:16:31.35 SS.hold @14:16:42.69 Intake @15.1psia, vacuum from 9.6 to 12.6psi @14:16:42.71 SS.reconfigure :inhaling 0.47ml/s @14:16:42.76 SS.seek 25ml @14:16:43.17 Stroke completed -- waiting for pressure to equalize ... @14:16:43.18 SS.reconfigure :exhale @14:16:43.23 SS.hold @14:16:54.41 Intake.select :close @14:16:54.56 SSV.dial :exhaust @14:16:55.31 Exhaust.select :open @14:16:55.42 SS.seek 0ml @14:17:04.45 Exhaust.select :close @14:17:04.56 SSV.dial CBV @14:17:04.58
CBV.dial SSV @14:17:05.36 Allowing 130s for 25.0ml of remaining 600.0ml @14:17:05.37 Intake.select :open @14:17:07.54 Intake @15.3psia, vacuum from 9.8 to 12.8psi @14:17:07.55 SS.reconfigure :inhaling 0.47ml/s @14:17:07.60 SS.seek 25ml @14:18:02.71 Stroke completed -- waiting for pressure to equalize ... @14:18:02.72 SS.reconfigure :exhale @14:18:02.77 SS.hold @14:18:25.43 Intake.select :close @14:18:25.58 SSV.dial :exhaust @14:18:26.32 Exhaust.select :open @14:18:26.44 SS.seek 0ml @14:18:35.23 Exhaust.select :close @14:18:35.34 SSV.dial CBV @14:18:35.36
CBV.dial SSV @14:18:36.15 Allowing 130s for 25.0ml of remaining 575.0ml @14:18:36.16 Intake.select :open @14:18:38.33 Intake @15.3psia, vacuum from 9.8 to 12.8psi @14:18:38.34 SS.reconfigure :inhaling 0.47ml/s @14:18:38.39 SS.seek 25ml @14:19:34.10 Stroke completed -- waiting for pressure to equalize ... @14:19:34.11 SS.reconfigure :exhale @14:19:34.16 SS.hold @14:20:01.18 Intake.select :close @14:20:01.33 SSV.dial :exhaust @14:20:02.09 Exhaust.select :open @14:20:02.20 SS.seek 0ml @14:20:11.27 Exhaust.select :close @14:20:11.39 SSV.dial CBV @14:20:11.41
CBV.dial SSV @14:20:12.22 Allowing 130s for 25.0ml of remaining 550.0ml @14:20:12.23 Intake.select :open @14:20:14.40 Intake @15.4psia, vacuum from 9.9 to 12.9psi @14:20:14.42 SS.reconfigure :inhaling 0.47ml/s @14:20:14.47 SS.seek 25ml @14:21:01.92 Vacuum too high -- waiting up to 40 seconds @14:21:01.93 SS.reconfigure :exhale @14:21:01.99 SS.hold @14:21:18.42 Intake @15.3psia, vacuum from 9.8 to 12.8psi @14:21:18.43 SS.reconfigure :inhaling 0.38ml/s @14:21:18.48 SS.seek 25ml @14:21:28.81 Stroke completed -- waiting for pressure to equalize ... @14:21:28.82 SS.reconfigure :exhale @14:21:28.87 SS.hold @14:21:49.28 Intake.select :close @14:21:50.08 SSV.dial :exhaust @14:21:50.84 Exhaust.select :open @14:21:50.95 SS.seek 0ml @14:21:59.71 Exhaust.select :close @14:21:59.82 SSV.dial CBV @14:21:59.86
CBV.dial SSV @14:22:00.59 Allowing 130s for 25.0ml of remaining 525.0ml @14:22:00.60 Intake.select :open @14:22:02.77 Intake @15.2psia, vacuum from 9.7 to 12.7psi @14:22:02.79 SS.reconfigure :inhaling 0.38ml/s @14:22:02.83 SS.seek 25ml @14:23:12.52 Stroke completed -- waiting for pressure to equalize ... @14:23:12.53 SS.reconfigure :exhale @14:23:12.58 SS.hold @14:23:43.50 Intake.select :close @14:23:43.65 SSV.dial :exhaust @14:23:44.40 Exhaust.select :open @14:23:44.51 SS.seek 0ml @14:23:53.57 Exhaust.select :close @14:23:53.68 SSV.dial CBV @14:23:53.70
CBV.dial SSV @14:23:54.46 Allowing 130s for 25.0ml of remaining 500.0ml @14:23:54.47 Intake.select :open @14:23:56.63 Intake @15.3psia, vacuum from 9.8 to 12.8psi @14:23:56.65 SS.reconfigure :inhaling 0.38ml/s @14:23:56.70 SS.seek 25ml @14:25:01.39 Vacuum too high -- waiting up to 40 seconds @14:25:01.40 SS.reconfigure :exhale @14:25:01.45 SS.hold @14:25:19.96 Intake @15.4psia, vacuum from 9.9 to 12.9psi @14:25:19.98 SS.reconfigure :inhaling 0.31ml/s @14:25:20.03 SS.seek 25ml @14:25:26.77 Stroke completed -- waiting for pressure to equalize ... @14:25:26.78 SS.reconfigure :exhale @14:25:26.82 SS.hold @14:25:49.74 Intake.select :close @14:25:49.90 SSV.dial :exhaust @14:25:50.64 Exhaust.select :open @14:25:50.76 SS.seek 0ml @14:25:59.58 Exhaust.select :close @14:25:59.69 SSV.dial CBV @14:25:59.72
CBV.dial SSV @14:26:00.47 Allowing 130s for 25.0ml of remaining 475.0ml @14:26:00.48 Intake.select :open @14:26:02.64 Intake @15.4psia, vacuum from 9.9 to 12.9psi @14:26:02.66 SS.reconfigure :inhaling 0.31ml/s @14:26:02.71 SS.seek 25ml @14:27:33.62 Stroke completed -- waiting for pressure to equalize ... @14:27:33.63 SS.reconfigure :exhale @14:27:33.68 SS.hold @14:28:10.46 Intake.select :close @14:28:10.62 SSV.dial :exhaust @14:28:11.38 Exhaust.select :open @14:28:11.49 SS.seek 0ml @14:28:20.27 Exhaust.select :close @14:28:20.38 SSV.dial CBV @14:28:20.40
CBV.dial SSV @14:28:21.17 Allowing 130s for 25.0ml of remaining 450.0ml @14:28:21.18 Intake.select :open @14:28:23.34 Intake @15.4psia, vacuum from 9.9 to 12.9psi @14:28:23.36 SS.reconfigure :inhaling 0.31ml/s @14:28:23.41 SS.seek 25ml @14:29:53.90 Stroke completed -- waiting for pressure to equalize ... @14:29:53.91 SS.reconfigure :exhale @14:29:53.96 SS.hold @14:30:31.19 SS.reconfigure :default @14:30:31.24 SS.hold @14:30:31.30 Intake.select :close @14:30:31.46 ShallowSampler::Clogged in MAIN -- Filtering too Slowly after 575.0ml @14:30:31.47 SSV.dial :exhaust @14:30:32.22 Exhaust.select :open @14:30:32.33 SS.reconfigure :exhale @14:30:32.38 SS.seek 0ml @14:30:41.20 Exhaust.select :close @14:30:41.31 Sampled 575.0ml @14:30:41.32 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @14:30:41.33 Intake.select :close @14:30:41.83 CSV.dial :waste @14:30:41.96 SSV.dial CBV,avoiding: :exhaust @14:30:41.99
CBV.dial SSV @14:30:42.12 CS.seek 0ml @14:30:42.20 CSV.dial :air @14:30:42.94 CS.seek 10ml @14:30:43.87
CTV.dial :air @14:30:44.62 delay 15 seconds @14:30:59.64 SSV.dial :spit,avoiding: :exhaust @14:31:00.96 CTV.dial CSV,avoiding: :intake @14:31:01.00
SS.seek 0ml @14:31:01.12 SSV.dial CBV,avoiding: :exhaust @14:31:07.51 SSV.dial between CBV and plug @14:31:08.00 CBV.dial between SSV and waste2 @14:31:08.40 CTV.dial CSV,avoiding: :air @14:31:08.42
CSV.dial CTV @14:31:08.89 CS.reconfigure :pressurize @14:31:08.94 CS.seek 0ml @14:31:27.49 BubblePoint: Intake @24.2psia, Outlet @0.3psia @14:31:27.50 CBV.dial SSV @14:31:27.83 SS.reconfigure :default @14:31:27.87 SS.seek 10ml @14:31:33.54 delay 15 seconds @14:31:48.68 Evacuated: Intake @23.9psia, Outlet @24.0psia @14:31:48.69 SSV.dial :spit,avoiding: :exhaust @14:31:49.74 SS.reconfigure :exhale @14:31:49.79 SS.seek 0ml @14:31:54.65 SS.reconfigure :fast @14:31:54.69 SS.seek 5ml @14:31:57.67 SSV.dial CBV,avoiding: :exhaust @14:31:57.70
CBV.dial SSV @14:31:59.06 CTV.dial :air,avoiding: :intake @14:32:00.40 CSV.dial CTV @14:32:00.44
SS.seek 10ml @14:32:01.65 CSV.dial :air @14:32:02.09 CS.reconfigure :fast @14:32:02.14 CS.seek 0ml @14:32:03.04
SSV.dial :spit,avoiding: :exhaust @14:32:04.36 SS.reconfigure :exhale @14:32:04.41 SS.seek 0ml @14:32:09.32 SS.reconfigure :fast @14:32:09.37 SS.seek 5ml @14:32:09.72 CS.stop @14:32:12.20 SSV.dial CBV,avoiding: :exhaust @14:32:12.23
CBV.dial SSV @14:32:14.98 SSV.dial :spit,avoiding: :exhaust @14:32:16.30 SS.reconfigure :default @14:32:16.35 SS.seek 0ml @14:32:19.60 SS.stop @14:32:19.64 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @14:32:19.65 Intake.select :close @14:32:19.73 CSV.dial :waste @14:32:19.85 SSV.dial CBV,avoiding: :exhaust @14:32:19.88
CBV.dial SSV @14:32:20.50 CS.seek 0ml @14:32:20.57 CSV.dial :air @14:32:21.20
CTV.dial :air @14:32:21.27 delay 15 seconds @14:32:21.32 CS.seek 10ml @14:32:36.30
SSV.dial :spit,avoiding: :exhaust @14:32:37.61 CTV.dial CSV,avoiding: :intake @14:32:37.64
SS.reconfigure :exhale @14:32:37.75 SS.seek 0ml @14:32:38.14 SSV.dial CBV,avoiding: :exhaust @14:32:45.73 SSV.dial between CBV and plug @14:32:46.22 CBV.dial between SSV and waste2 @14:32:46.61 CSV.dial between air and CTV @14:32:46.92 CS.seek 7.508ml @14:32:53.39 CTV.dial CSV,avoiding: :air @14:32:53.41
CSV.dial CTV @14:32:53.73 CS.reconfigure :pressurize @14:32:53.78 CS.seek 0ml @14:33:09.36 BubblePoint: Intake @24.2psia, Outlet @3.4psia @14:33:09.37 CBV.dial SSV @14:33:09.70 SS.reconfigure :default @14:33:09.75 SS.seek 10ml @14:33:15.34 delay 15 seconds @14:33:30.47 Evacuated: Intake @24.0psia, Outlet @24.1psia @14:33:30.49 SSV.dial :spit,avoiding: :exhaust @14:33:31.52 SS.reconfigure :exhale @14:33:31.57 SS.seek 0ml @14:33:36.61 SS.reconfigure :fast @14:33:36.66 SS.seek 5ml @14:33:39.66 SSV.dial CBV,avoiding: :exhaust @14:33:39.68
CBV.dial SSV @14:33:41.04 CTV.dial :air,avoiding: :intake @14:33:42.38 CSV.dial CTV @14:33:42.42
SS.seek 10ml @14:33:43.64 CSV.dial :air @14:33:44.07 CS.reconfigure :fast @14:33:44.12 CS.seek 0ml @14:33:45.41
SSV.dial :spit,avoiding: :exhaust @14:33:46.80 SS.reconfigure :exhale @14:33:46.85 SS.seek 0ml @14:33:48.72 CS.stop @14:33:51.96
SS.reconfigure :fast @14:33:52.01 SS.seek 5ml @14:33:54.83 SSV.dial CBV,avoiding: :exhaust @14:33:54.86
CBV.dial SSV @14:33:57.21 SSV.dial :spit,avoiding: :exhaust @14:33:58.53 SS.reconfigure :default @14:33:58.58 SS.seek 0ml @14:34:01.81 SS.stop @14:34:01.85 Lysing: Adding 2.0ml AqMeOH @14:34:01.86 CBV.dial between CSV and waste1,avoiding: :DBV @14:34:03.35 CTV.dial CSV @14:34:04.66 CSV.dial CSR @14:34:05.45 Pulling 1ml of CSR.AqMeOH @14:34:05.46 CSR.select :AqMeOH @14:34:05.58 CS.reconfigure :default @14:34:05.63 CS.seek 1ml @14:34:09.01 delay 5 seconds @14:34:14.03 CSR.select :air @14:34:14.19 CS.seek 6ml @14:34:27.79 delay 2 seconds @14:34:29.80 CSV.dial :waste2 @14:34:30.24 CS.reconfigure :fast @14:34:30.29 CS.seek 0ml @14:34:44.63 CSV.dial CSR @14:34:45.08 Pulling 2ml of CSR.AqMeOH @14:34:45.09 CSR.select :AqMeOH @14:34:45.21 CS.reconfigure :default @14:34:45.25 CS.seek 2ml @14:34:51.13 delay 5 seconds @14:34:56.15 CSR.select :air @14:34:56.32 CS.seek 4.999ml @14:35:04.97 delay 2 seconds @14:35:06.99 CSV.dial CTV @14:35:07.43 CS.seek 1ml @14:35:18.61 Before lysing Collection Puck Heater at 17.1C/29.9psia @14:35:18.63 Heating Puck.from 2, :sh1 in Collection Clamp to 70C for 10:00 @14:35:18.65 CH.seek 70C,8:00 @14:37:25.08 CH.hold 10:00,70C @14:37:25.28 Begun lysing Collection Puck Heater at 68C/36.0psia @14:47:25.28 Ended lysing Collection Puck Heater at 70C/35.2psia @14:47:25.29 CS.seek 0ml @14:47:28.78 CTV.dial between spare and air,avoiding: :intake @14:47:29.89 CSV.dial :air @14:47:30.34 CS.seek 5ml @14:47:43.93 CSV.dial CTV @14:47:44.39 delay 2 seconds @14:47:46.40 CSV.dial :air @14:47:46.85 delay 2 seconds @14:47:48.87 CSV.dial CTV @14:47:49.32 delay 2 seconds @14:47:51.34 CSV.dial :air @14:47:51.79 delay 2 seconds @14:47:53.81 CS.seek 0ml @14:48:07.45 Puck @80.7psia after final lysis pressurization @14:48:07.47 Recovering Lysate into Collection syringe @14:48:07.48 CSV.dial CBV @14:48:08.60 CBV.dial CSV @14:48:09.00 CS.seek 5ml @14:48:22.68 delay 5 seconds @14:48:27.70 CTV.dial :air @14:48:28.23 CS.seek 7ml @14:48:34.21 delay 5 seconds @14:48:39.23 CSV.dial :air2 @14:48:39.69 CS.seek 10ml @14:48:48.53 CSV.dial between air and CTV,avoiding: CBV @14:48:49.67 Lysing Complete @14:48:49.74 Storing sh1 puck in Collection Clamp to tube 1 @14:48:49.92 SC.reconfigure :tight @14:48:49.97 SC.seek 1 @14:48:53.51 SC.configure :limp @14:48:53.74 Elbow.reconfigure :tight @14:48:53.79 Elbow.seek 1 @14:48:55.60 Forearm.reconfigure :tight @14:48:55.64 Forearm.seek 1 + 450 counts @14:48:56.66 SE.reconfigure :pick @14:48:56.72 SE.seek :beamBreak @14:48:59.19 SE.configure :default @14:48:59.35 Elbow.reconfigure :limp @14:48:59.40 Forearm.reconfigure :loose @14:48:59.45 Forearm.seek 1 @14:48:59.66 Elbow.hold @14:48:59.72 Elbow.reconfigure :tight @14:49:00.26 SE.seek 11.08 Pucks below Top Plate @14:49:00.79 Forearm.force -20 @14:49:00.83 Forearm.seek 1 + 305 counts @14:49:01.13 Forearm.reconfigure :tight @14:49:01.18 Forearm.seek 1 + 450 counts @14:49:01.37 SE.seek 12.08 Pucks below Top Plate @14:49:01.95 Forearm.reconfigure :loose @14:49:01.99 Forearm.seek :retract @14:49:02.91 Elbow.seek CC @14:49:04.60 Elbow.reconfigure :loose @14:49:04.71 Forearm.seek CC @14:49:05.69 Hand.close @14:49:06.27 CC.seek :open @14:49:11.01 Forearm.seek :retract @14:49:11.99 Elbow.reconfigure :tight @14:49:12.04 Elbow.seek 1 @14:49:13.81 Elbow.reconfigure :limp @14:49:13.86 Forearm.seek 1 @14:49:14.86 Elbow.hold @14:49:14.93 Elbow.reconfigure :tight @14:49:14.98 Hand.open @14:49:15.76 Forearm.force -20 @14:49:15.81 Forearm.seek 1 + 312 counts @14:49:16.11 Forearm.reconfigure :tight @14:49:16.16 Forearm.seek 1 + 450 counts @14:49:16.41 SE.seek :down @14:49:17.55
Forearm.reconfigure :loose @14:49:17.60 Forearm.seek :retract @14:49:18.29 SE.stop @14:49:18.98
Tranferring DA @14:49:18.99 Beginning samplePassC @14:49:19.00 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:49:19.20 Elbow.seek FlushPuck::Garage @14:49:20.94 Elbow.reconfigure :loose @14:49:21.05 Forearm.seek :garage @14:49:22.00 Hand.close @14:49:22.77 Forearm.seek :retract @14:49:23.76 Elbow.reconfigure :tight @14:49:23.81 Elbow.seek CC @14:49:26.67 Elbow.reconfigure :loose @14:49:26.77 Forearm.reconfigure :tight @14:49:26.82 Forearm.seek CC @14:49:28.05 CC.seek :closed @14:49:32.58 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.36 @14:49:32.64 Hand.open @14:49:33.33 Forearm.reconfigure :loose @14:49:33.38 Forearm.seek :retract @14:49:34.38 PSV.dial PSR @14:49:35.76 Pulling 1.5ml of PSR.flush @14:49:35.77 PSR.select :flush @14:49:35.89 PS.seek 1.5ml @14:49:52.09 delay 5 seconds @14:49:57.11 PSV.dial :waste1 @14:49:57.58 PS.seek 0ml @14:50:14.34 PSV.dial PSR @14:50:14.81 PSR.select :air @14:50:14.93 PS.seek 2.5ml @14:50:41.25 PRV.dial CBV,avoiding: :ab2 @14:50:41.28
CBV.dial PRV @14:50:43.03 PSV.dial :mixloop @14:50:44.06 CTV.dial :air,avoiding: :intake @14:50:44.15 PTV.dial :PRVmixing @14:50:45.46 PS.seek 0ml @14:51:11.84 PSV.dial :air @14:51:12.32 PS.seek 2.5ml @14:51:38.62 PSV.dial :mixloop @14:51:39.10 PS.seek 0ml @14:52:05.43 PSV.dial :air @14:52:05.91 PS.seek 2.5ml @14:52:32.24 PSV.dial :mixloop @14:52:32.72 PS.seek 0ml @14:52:59.12 Garaging FlushPuck in Collection Clamp @14:52:59.20 Elbow.reconfigure :tight @14:52:59.24 Elbow.seek CC @14:52:59.60 Elbow.reconfigure :loose @14:52:59.71 Forearm.seek CC @14:53:00.75 Hand.close @14:53:01.40 CC.seek :open @14:53:06.05 Forearm.seek :retract @14:53:07.05 Elbow.reconfigure :tight @14:53:07.10 Elbow.seek :garage @14:53:09.95 Elbow.reconfigure :loose @14:53:10.05 Forearm.reconfigure :tight @14:53:10.10 Forearm.seek :garage @14:53:11.16 Hand.open @14:53:11.83 Forearm.reconfigure :loose @14:53:11.87 Forearm.seek :retract @14:53:12.86 PSV.dial :mixloop @14:53:12.93 PTV.dial :PRVmixing @14:53:12.95 PRV.dial CBV @14:53:13.35
CBV.dial PRV @14:53:13.63 CTV.dial :air,avoiding: :intake @14:53:13.71 Loading lyfil puck from tube 2 into Collection Clamp @14:53:13.96 SC.reconfigure :tight @14:53:14.01 SC.seek 2 @14:53:17.57 SC.configure :limp @14:53:17.80 Elbow.reconfigure :tight @14:53:17.85 Elbow.seek 2 @14:53:19.76 Forearm.reconfigure :tight @14:53:19.81 Forearm.seek 2 + 450 counts @14:53:20.89 SE.reconfigure :pick @14:53:20.95 SE.seek :beamBreak @14:53:22.85 SE.configure :default @14:53:23.01 Elbow.seek 2 @14:53:23.21 Forearm.seek 2 + 300 counts @14:53:23.45 Forearm.reconfigure :loose @14:53:23.50 Hand.close @14:53:24.16 SE.seek :down @14:53:24.36
Forearm.seek :retract @14:53:25.30 Elbow.seek CC @14:53:25.63 SE.stop @14:53:26.90
Elbow.reconfigure :loose @14:53:27.01 Forearm.reconfigure :tight @14:53:27.05 Forearm.seek CC @14:53:28.79 CC.seek :closed @14:53:33.37 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.18 @14:53:33.39 Hand.open @14:53:34.08 Forearm.reconfigure :loose @14:53:34.13 Forearm.seek :retract @14:53:35.16 CTV.dial CSV,avoiding: :intake @14:53:36.52 CSV.dial CTV @14:53:36.84 PS.seek 2.5ml @14:54:03.31 CS.seek 4.5ml @14:54:18.15 delay 8 seconds @14:54:26.17 PSV.dial :air @14:54:26.64 delay 2 seconds @14:54:28.65 PSV.dial :waste2 @14:54:29.39 PS.seek 0ml @14:54:55.78 CBV.dial between PRV and waste1 @14:54:56.11 CSV.dial :air @14:54:56.57 CS.seek 10ml @14:55:11.55 CSV.dial CTV @14:55:12.01 CS.seek 0ml @14:55:38.61 CBV.dial :waste1 @14:55:38.91 delay 5 seconds @14:55:43.93 CTV.dial :air,avoiding: :intake @14:55:45.36 Storing lyfil puck in Collection Clamp to tube 1 @14:55:45.54 SC.reconfigure :tight @14:55:45.59 SC.seek 1 @14:55:49.24 SC.configure :limp @14:55:49.47 Elbow.reconfigure :tight @14:55:49.51 Elbow.seek 1 @14:55:51.34 Forearm.reconfigure :tight @14:55:51.39 Forearm.seek 1 + 450 counts @14:55:52.54 SE.reconfigure :pick @14:55:52.60 SE.seek :beamBreak @14:55:54.96 SE.configure :default @14:55:55.12 Elbow.reconfigure :limp @14:55:55.17 Forearm.reconfigure :loose @14:55:55.22 Forearm.seek 1 @14:55:55.50 Elbow.hold @14:55:55.57 Elbow.reconfigure :tight @14:55:55.67 SE.seek 12.09 Pucks below Top Plate @14:55:56.14 Forearm.force -20 @14:55:56.18 Forearm.seek 1 + 310 counts @14:55:56.47 Forearm.reconfigure :tight @14:55:56.52 Forearm.seek 1 + 450 counts @14:55:56.71 SE.seek 13.09 Pucks below Top Plate @14:55:57.26 Forearm.reconfigure :loose @14:55:57.31 Forearm.seek :retract @14:55:58.22 Elbow.seek CC @14:55:59.88 Elbow.reconfigure :loose @14:56:00.00 Forearm.seek CC @14:56:00.97 Hand.close @14:56:01.64 CC.seek :open @14:56:06.38 Forearm.seek :retract @14:56:07.41 Elbow.reconfigure :tight @14:56:07.46 Elbow.seek 1 @14:56:09.25 Elbow.reconfigure :limp @14:56:09.31 Forearm.seek 1 @14:56:10.34 Elbow.hold @14:56:10.84 Elbow.reconfigure :tight @14:56:10.89 Hand.open @14:56:11.65 Forearm.force -20 @14:56:11.69 Forearm.seek 1 + 319 counts @14:56:12.00 Forearm.reconfigure :tight @14:56:12.05 Forearm.seek 1 + 450 counts @14:56:12.30 SE.seek :down @14:56:13.44
Forearm.reconfigure :loose @14:56:13.49 Forearm.seek :retract @14:56:14.12 SE.stop @14:56:14.41
PSV.dial :air @14:56:15.16 PS.seek 2.5ml @14:56:41.49 delay 1 second @14:56:42.51 PSV.dial :mixloop @14:56:42.96 delay 1 second @14:56:43.98 PSV.dial :air @14:56:44.57 delay 1 second @14:56:45.59 PSV.dial :mixloop @14:56:46.04 delay 1 second @14:56:47.06 PSV.dial :air @14:56:47.52 delay 1 second @14:56:48.55 PS.seek 0ml @14:57:14.93 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @14:57:15.12 Elbow.seek FlushPuck::Garage @14:57:16.95 Elbow.reconfigure :loose @14:57:17.05 Forearm.seek :garage @14:57:18.05 Hand.close @14:57:18.76 Forearm.seek :retract @14:57:19.77 Elbow.reconfigure :tight @14:57:19.81 Elbow.seek PC @14:57:21.07 Elbow.reconfigure :loose @14:57:21.18 Forearm.reconfigure :tight @14:57:21.22 Forearm.seek PC @14:57:22.46 PC.seek :closed @14:57:27.10 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.60 @14:57:27.16 Hand.open @14:57:27.83 Forearm.reconfigure :loose @14:57:27.88 Forearm.seek :retract @14:57:28.84 PRV.dial :air @14:57:29.29 PSV.dial :mixloop @14:57:29.82 PS.seek 0.3ml @14:57:33.77 PSV.dial PSR @14:57:34.82 Pulling 0.4ml of PSR.air @14:57:34.83 PSR.select :air @14:57:34.85 PS.seek 0.7ml @14:57:39.80 delay 3 seconds @14:57:42.82 PSR.select :air @14:57:42.84 PS.seek 2.5ml @14:58:02.09 delay 3 seconds @14:58:05.10 PSV.dial :mixloop @14:58:06.15 PTV.dial :mixing @14:58:06.90 PBV.dial :waste2 @14:58:07.86 PS.seek 0ml @14:58:34.30 Garaging FlushPuck in Processing Clamp @14:58:34.37 Elbow.reconfigure :tight @14:58:34.42 Elbow.seek PC @14:58:34.54 Elbow.reconfigure :loose @14:58:34.65 Forearm.seek PC @14:58:35.59 Hand.close @14:58:36.14 PC.seek :open @14:58:40.86 Forearm.seek :retract @14:58:41.81 Elbow.reconfigure :tight @14:58:41.86 Elbow.seek :garage @14:58:43.28 Elbow.reconfigure :loose @14:58:43.38 Forearm.reconfigure :tight @14:58:43.43 Forearm.seek :garage @14:58:44.45 Hand.open @14:58:45.08 Forearm.reconfigure :loose @14:58:45.12 Forearm.seek :retract @14:58:46.06 Loading da puck from tube 2 into Processing Clamp @14:58:46.30 SC.reconfigure :tight @14:58:46.35 SC.seek 2 @14:58:49.93 SC.configure :limp @14:58:50.15 Elbow.reconfigure :tight @14:58:50.19 Elbow.seek 2 @14:58:52.18 Forearm.reconfigure :tight @14:58:52.23 Forearm.seek 2 + 450 counts @14:58:53.28 SE.reconfigure :pick @14:58:53.34 SE.seek :beamBreak @14:58:55.22 SE.configure :default @14:58:55.38 Elbow.seek 2 @14:58:55.57 Forearm.seek 2 + 300 counts @14:58:55.75 Forearm.reconfigure :loose @14:58:55.79 Hand.close @14:58:56.40 SE.seek :down @14:58:56.61
Forearm.seek :retract @14:58:57.59 Elbow.seek PC @14:58:57.96 SE.stop @14:59:00.66
Elbow.reconfigure :loose @14:59:00.77 Forearm.reconfigure :tight @14:59:00.82 Forearm.seek PC @14:59:02.51 PC.seek :closed @14:59:07.16 Processing Clamp onSpring/offSpring for 16/26 samples, on/off torque 1.63 @14:59:07.17 Hand.open @14:59:07.83 Forearm.reconfigure :loose @14:59:07.88 Forearm.seek :retract @14:59:08.83 Heating Puck.from 2, :da in Processing Clamp to 28C for 2:00:00 @14:59:08.85 PH.seek 28C,5:00 @14:59:49.48 PH.hold 2:00:00,28C @14:59:49.53 delay 1:30 @15:01:19.57 PSR.select :air @15:01:19.59 PSV.dial PSR @15:01:20.63 Pulling 0.5ml of PSR.wash @15:01:20.64 PSR.select :wash @15:01:20.76 PS.seek 0.5ml @15:01:26.86 delay 5 seconds @15:01:31.88 PSR.select :air @15:01:32.00 PS.seek 2ml @15:01:48.14 PTV.dial :puck @15:01:48.88 PSV.dial PTV @15:01:49.64 PBV.dial :waste1 @15:01:51.03 PS.seek 1.5ml @15:01:56.93 delay 3 seconds @15:01:59.94 PBV.dial between PSV and waste1 @15:02:00.31 PS.seek 0ml @15:02:16.49 delay 5:00 @15:07:16.51 recoverPSRreagent08 @15:07:16.52 PTV.dial :puck @15:07:16.53 PBV.dial :waste1 @15:07:16.90 delay 30 seconds @15:07:46.92 PBV.dial between PSV and waste1 @15:07:47.28 PSR.select :air @15:07:47.29 PSV.dial PSR @15:07:48.05 PS.reconfigure :fast @15:07:48.10 PS.seek 2.5ml @15:08:11.95 PSV.dial PTV @15:08:12.70 delay 5 seconds @15:08:17.72 PSV.dial PSR @15:08:18.46 delay 5 seconds @15:08:23.48 PSV.dial PTV @15:08:24.23 delay 5 seconds @15:08:29.25 PSV.dial :air @15:08:30.00 PS.seek 0ml @15:08:53.32 PTV.dial :PRVmixing,via: PRV @15:08:54.63 PSV.dial :mixloop @15:08:55.10 PRV.dial :ab1 @15:08:55.86 PS.reconfigure :slow @15:08:55.91 PS.seek 0.15ml @15:08:59.45 delay 2 seconds @15:09:01.47 CBV.dial PRV @15:09:01.49
PRV.dial CBV @15:09:02.06 PS.seek 0.35ml @15:09:06.82 delay 2 seconds @15:09:08.84 CBV.dial between PRV and PSV @15:09:09.16 PRV.dial :air @15:09:09.61 PS.seek 0.9ml @15:09:21.41 PRV.dial :waste2 @15:09:22.17 PS.seek 0.5ml @15:09:30.88 addPMLReagent08 @15:09:30.89 PSV.dial :air @15:09:31.80 PS.reconfigure :default @15:09:31.84 PS.seek 2ml @15:09:48.04 PSV.dial :PML @15:09:48.51 PTV.dial :mixing @15:09:49.26 PBV.dial :waste1 @15:09:49.68 PS.seek 1.85ml @15:09:52.01 PBV.dial between PSV and waste1 @15:09:52.37 PS.seek 0ml @15:10:12.07 delay 2 seconds @15:10:14.09 Beginning samplePassC @15:10:14.10 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @15:10:14.29 Elbow.reconfigure :tight @15:10:14.34 Elbow.seek FlushPuck::Garage @15:10:15.73 Elbow.reconfigure :loose @15:10:15.83 Forearm.seek :garage @15:10:16.79 Hand.close @15:10:17.51 Forearm.seek :retract @15:10:18.48 Elbow.reconfigure :tight @15:10:18.52 Elbow.seek CC @15:10:21.40 Elbow.reconfigure :loose @15:10:21.51 Forearm.reconfigure :tight @15:10:21.55 Forearm.seek CC @15:10:22.81 CC.seek :closed @15:10:27.35 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.43 @15:10:27.42 Hand.open @15:10:28.49 Forearm.reconfigure :loose @15:10:28.58 Forearm.seek :retract @15:10:29.53 PSV.dial PSR @15:10:30.59 Pulling 1.5ml of PSR.flush PSR.select :flush @15:10:30.72 PS.seek 1.5ml @15:10:46.82 delay 5 seconds @15:10:51.84 PSV.dial :waste1 @15:10:52.31 PS.seek 0ml @15:11:08.50 PSV.dial PSR @15:11:08.97 PSR.select :air @15:11:09.09 PS.seek 2.5ml @15:11:35.45 PRV.dial CBV,avoiding: :ab2 @15:11:35.47
CBV.dial PRV @15:11:37.09 PSV.dial :mixloop @15:11:38.12 CTV.dial :air,avoiding: :intake @15:11:38.19 PTV.dial :PRVmixing @15:11:38.95 PS.seek 0ml @15:12:05.25 PSV.dial :air @15:12:05.84 PS.seek 2.5ml @15:12:32.19 PSV.dial :mixloop @15:12:32.65 PS.seek 0ml @15:12:59.06 PSV.dial :air @15:12:59.54 PS.seek 2.5ml @15:13:25.94 PSV.dial :mixloop @15:13:26.42 PS.seek 0ml @15:13:52.75 Garaging FlushPuck in Collection Clamp @15:13:52.83 Elbow.reconfigure :tight @15:13:52.88 Elbow.seek CC @15:13:53.18 Elbow.reconfigure :loose @15:13:53.29 Forearm.seek CC @15:13:54.31 Hand.close @15:13:54.88 CC.seek :open @15:13:59.50 Forearm.seek :retract @15:14:00.49 Elbow.reconfigure :tight @15:14:00.54 Elbow.seek :garage @15:14:03.42 Elbow.reconfigure :loose @15:14:03.52 Forearm.reconfigure :tight @15:14:03.94 Forearm.seek :garage @15:14:05.01 Hand.open @15:14:05.69 Forearm.reconfigure :loose @15:14:05.75 Forearm.seek :retract @15:14:06.71 delayUntil "15:18:14.08" @15:14:06.73 Whole Cell Archive sampling 1000ml @15:14:06.75 Loading wcr puck from tube 2 into Collection Clamp @15:14:07.00 SC.reconfigure :tight @15:14:07.05 SC.seek 2 @15:14:07.45 SC.configure :limp @15:14:07.68 Elbow.reconfigure :tight @15:14:07.73 Elbow.seek 2 @15:14:09.66 Forearm.reconfigure :tight @15:14:09.71 Forearm.seek 2 + 450 counts @15:14:10.77 SE.reconfigure :pick @15:14:10.83 SE.seek :beamBreak @15:14:12.89 SE.configure :default @15:14:13.06 Elbow.seek 2 @15:14:13.25 Forearm.seek 2 + 300 counts @15:14:13.41 Forearm.reconfigure :loose @15:14:13.46 Hand.close @15:14:14.14 SE.seek :down @15:14:14.35 Forearm.seek :retract @15:14:15.30 Elbow.seek CC @15:14:15.78 SE.stop @15:14:16.92 Elbow.reconfigure :loose @15:14:17.02 Forearm.reconfigure :tight @15:14:17.45 Forearm.seek CC @15:14:18.82 CC.seek :closed @15:14:23.35 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.29 @15:14:23.36 Hand.open @15:14:24.07 Forearm.reconfigure :loose @15:14:24.12 Forearm.seek :retract @15:14:25.13 collectSample 1000ml w/bubble point of 35psi @15:14:25.14 Priming 7ml @15:14:25.15 Intake.select :close @15:14:25.16 Exhaust.select :close @15:14:25.18 SS.hold @15:14:25.38 Calibrating pressure sensors @15:14:25.40 CTV.dial :air @15:14:25.47 SSV.dial :spit,avoiding: :exhaust @15:14:25.56 SS.reconfigure :fast @15:14:25.62 SS.seek 5ml @15:14:28.29 SSV.dial CBV @15:14:28.32 CBV.dial SSV @15:14:29.68 delay 3.8 seconds @15:14:33.62 Sensor limits: Intake max 117.4psia; Outlet max 109.7psia @15:14:33.63 SSV.dial :spit @15:14:34.96 SS.seek 0ml @15:14:37.78 SSV.dial CBV @15:14:39.11 CTV.dial :intake @15:14:39.88 SSV.dial CBV @15:14:39.91 CBV.dial SSV @15:14:40.05 Allowing 40s for 7.0ml of remaining 7.0ml @15:14:40.06 Intake.select :open @15:14:42.23 Intake @14.8psia, vacuum from 9.3 to 12.3psi @15:14:42.25 SS.reconfigure :inhaling 2.20ml/s @15:14:42.30 SS.seek 7ml @15:14:48.91 Stroke completed -- waiting for pressure to equalize ... @15:14:48.93 SS.reconfigure :exhale @15:14:49.40 SS.hold @15:14:58.01 Intake.select :close @15:14:58.17 SSV.dial :exhaust @15:14:58.95 Exhaust.select :open @15:14:59.06 SS.seek 0ml @15:15:02.98 Exhaust.select :close @15:15:03.09 Sampling 1000.0ml @15:15:03.10 Intake.select :close @15:15:03.11 Exhaust.select :close @15:15:03.12 SS.reconfigure :default @15:15:03.17 SS.hold @15:15:03.38 CTV.dial :intake @15:15:03.55 SSV.dial CBV @15:15:03.59 CBV.dial SSV @15:15:04.34 Allowing 130s for 25.0ml of remaining 1000.0ml @15:15:04.35 Intake.select :open @15:15:06.51 Intake @15.5psia, vacuum from 10.0 to 13.0psi @15:15:06.53 SS.reconfigure :inhaling 2.20ml/s @15:15:06.58 SS.seek 25ml @15:15:21.78 Stroke completed -- waiting for pressure to equalize ... @15:15:21.79 SS.reconfigure :exhale @15:15:22.28 SS.hold @15:15:42.75 Intake.select :close @15:15:42.91 SSV.dial :exhaust @15:15:43.69 Exhaust.select :open @15:15:43.80 SS.seek 0ml @15:15:52.54 Exhaust.select :close @15:15:52.65 SSV.dial CBV @15:15:52.68 CBV.dial SSV @15:15:53.46 Allowing 130s for 25.0ml of remaining 975.0ml @15:15:53.48 Intake.select :open @15:15:55.65 Intake @15.8psia, vacuum from 10.0 to 13.0psi @15:15:55.67 SS.reconfigure :inhaling 2.20ml/s @15:15:55.72 SS.seek 25ml @15:16:07.86 Vacuum too high -- waiting up to 40 seconds @15:16:07.88 SS.reconfigure :exhale @15:16:07.93 SS.hold @15:16:13.96 Intake @15.4psia, vacuum from 9.9 to 12.9psi @15:16:13.98 SS.reconfigure :inhaling 1.76ml/s @15:16:14.03 SS.seek 25ml @15:16:17.91 Stroke completed -- waiting for pressure to equalize ... @15:16:17.93 SS.reconfigure :exhale @15:16:17.97 SS.hold @15:16:30.72 Intake.select :close @15:16:30.88 SSV.dial :exhaust @15:16:31.65 Exhaust.select :open @15:16:31.76 SS.seek 0ml @15:16:40.76 Exhaust.select :close @15:16:40.87 SSV.dial CBV @15:16:40.90 CBV.dial SSV @15:16:41.68 Allowing 130s for 25.0ml of remaining 950.0ml @15:16:41.69 Intake.select :open @15:16:43.86 Intake @15.9psia, vacuum from 10.0 to 13.0psi @15:16:43.87 SS.reconfigure :inhaling 1.76ml/s @15:16:43.92 SS.seek 25ml @15:16:58.26 Vacuum too high -- waiting up to 40 seconds @15:16:58.28 SS.reconfigure :exhale @15:16:58.34 SS.hold @15:17:03.51 Intake @15.4psia, vacuum from 9.9 to 12.9psi @15:17:03.53 SS.reconfigure :inhaling 1.41ml/s @15:17:03.58 SS.seek 25ml @15:17:06.96 Stroke completed -- waiting for pressure to equalize ... @15:17:06.97 SS.reconfigure :exhale @15:17:07.02 SS.hold @15:17:18.18 Intake.select :close @15:17:18.34 SSV.dial :exhaust @15:17:19.12 Exhaust.select :open @15:17:19.23 SS.seek 0ml @15:17:28.19 Exhaust.select :close @15:17:28.31 SSV.dial CBV @15:17:28.35 CBV.dial SSV @15:17:29.54 Allowing 130s for 25.0ml of remaining 925.0ml @15:17:29.55 Intake.select :open @15:17:31.72 Intake @15.8psia, vacuum from 10.0 to 13.0psi @15:17:31.74 SS.reconfigure :inhaling 1.41ml/s @15:17:31.79 SS.seek 25ml @15:17:52.00 Stroke completed -- waiting for pressure to equalize ... @15:17:52.02 SS.reconfigure :exhale @15:17:52.07 SS.hold @15:18:06.48 Intake.select :close @15:18:06.64 SSV.dial :exhaust @15:18:07.40 Exhaust.select :open @15:18:07.51 SS.seek 0ml @15:18:14.09 recoverPRVreagent08 @15:18:14.10 PTV.dial :mixing @15:18:14.92 PBV.dial :waste1 @15:18:15.30 delay 30 seconds @15:18:16.52 Exhaust.select :close @15:18:16.64 SSV.dial CBV @15:18:16.66 CBV.dial SSV @15:18:17.46 Allowing 130s for 25.0ml of remaining 900.0ml @15:18:17.47 Intake.select :open @15:18:19.65 Intake @15.8psia, vacuum from 10.0 to 13.0psi @15:18:19.67 SS.reconfigure :inhaling 1.41ml/s @15:18:19.72 SS.seek 25ml @15:18:39.88 Stroke completed -- waiting for pressure to equalize ... @15:18:39.89 SS.reconfigure :exhale @15:18:39.95 SS.hold @15:18:45.31 PBV.dial between PSV and waste1 @15:18:45.68 PSV.dial :air @15:18:46.16 PS.reconfigure :fast @15:18:46.21 PS.seek 2.5ml @15:18:54.72 Intake.select :close @15:18:54.89 SSV.dial :exhaust @15:18:55.65 Exhaust.select :open @15:18:55.76 SS.seek 0ml @15:19:04.71 Exhaust.select :close @15:19:04.82 SSV.dial CBV @15:19:04.86 CBV.dial SSV @15:19:05.60 Allowing 130s for 25.0ml of remaining 875.0ml @15:19:05.61 Intake.select :open @15:19:07.78 Intake @15.8psia, vacuum from 10.0 to 13.0psi @15:19:07.80 SS.reconfigure :inhaling 1.41ml/s @15:19:07.85 SS.seek 25ml @15:19:10.25 PSV.dial :mixloop @15:19:10.72 delay 2 seconds @15:19:12.73 PSV.dial :air @15:19:13.20 delay 2 seconds @15:19:15.22 PSV.dial :mixloop @15:19:15.69 delay 2 seconds @15:19:17.70 PSV.dial :air @15:19:18.17 PS.seek 0ml @15:19:25.17 Vacuum too high -- waiting up to 40 seconds @15:19:25.19 SS.reconfigure :exhale @15:19:25.24 SS.hold @15:19:31.07 Intake @15.4psia, vacuum from 9.9 to 12.9psi @15:19:31.09 SS.reconfigure :inhaling 1.13ml/s @15:19:31.14 SS.seek 25ml @15:19:34.86 Stroke completed -- waiting for pressure to equalize ... @15:19:34.87 SS.reconfigure :exhale @15:19:35.30 SS.hold @15:19:41.78 washCycleDA #1 of 2 @15:19:41.79 PSR.select :air @15:19:41.80 PSV.dial PSR @15:19:42.94 Intake.select :close @15:19:43.10 SSV.dial :exhaust @15:19:43.18 Pulling 0.5ml of PSR.wash @15:19:43.19 PSR.select :wash @15:19:43.31 PS.reconfigure :default @15:19:43.36 PS.seek 0.5ml @15:19:43.88 Exhaust.select :open @15:19:44.00 SS.seek 0ml @15:19:49.31 delay 5 seconds @15:19:52.78 Exhaust.select :close @15:19:52.90 SSV.dial CBV @15:19:52.94 CBV.dial SSV @15:19:53.69 Allowing 130s for 25.0ml of remaining 850.0ml @15:19:53.71 Intake.select :open @15:19:54.32 PSR.select :air @15:19:54.45 PS.seek 2ml @15:19:55.87 Intake @15.8psia, vacuum from 10.0 to 13.0psi @15:19:55.89 SS.reconfigure :inhaling 1.13ml/s @15:19:55.94 SS.seek 25ml @15:20:10.59 PTV.dial :puck @15:20:11.34 PSV.dial PTV @15:20:12.54 PBV.dial :waste1 @15:20:12.98 PS.seek 1.5ml @15:20:18.96 delay 3 seconds @15:20:20.67 Stroke completed -- waiting for pressure to equalize ... @15:20:20.69 SS.reconfigure :exhale @15:20:20.74 SS.hold @15:20:22.01 PBV.dial between PSV and waste1 @15:20:22.40 PS.seek 0ml @15:20:33.43 Intake.select :close @15:20:33.59 SSV.dial :exhaust @15:20:34.37 Exhaust.select :open @15:20:34.49 SS.seek 0ml @15:20:38.59 delay 2:00 @15:20:43.50 Exhaust.select :close @15:20:43.62 SSV.dial CBV @15:20:43.64 CBV.dial SSV @15:20:44.43 Allowing 130s for 25.0ml of remaining 825.0ml @15:20:44.44 Intake.select :open @15:20:46.61 Intake @15.7psia, vacuum from 10.0 to 13.0psi @15:20:46.63 SS.reconfigure :inhaling 1.13ml/s @15:20:46.68 SS.seek 25ml @15:21:10.95 Stroke completed -- waiting for pressure to equalize ... @15:21:10.97 SS.reconfigure :exhale @15:21:11.02 SS.hold @15:21:27.29 Intake.select :close @15:21:27.45 SSV.dial :exhaust @15:21:28.22 Exhaust.select :open @15:21:28.34 SS.seek 0ml @15:21:37.13 Exhaust.select :close @15:21:37.25 SSV.dial CBV @15:21:37.28 CBV.dial SSV @15:21:38.06 Allowing 130s for 25.0ml of remaining 800.0ml @15:21:38.08 Intake.select :open @15:21:40.25 Intake @15.6psia, vacuum from 10.0 to 13.0psi @15:21:40.27 SS.reconfigure :inhaling 1.13ml/s @15:21:40.32 SS.seek 25ml @15:22:01.92 Vacuum too high -- waiting up to 40 seconds @15:22:01.93 SS.reconfigure :exhale @15:22:01.99 SS.hold @15:22:10.78 Intake @15.2psia, vacuum from 9.7 to 12.7psi @15:22:10.80 SS.reconfigure :inhaling 0.91ml/s @15:22:10.85 SS.seek 25ml @15:22:14.97 Stroke completed -- waiting for pressure to equalize ... @15:22:14.98 SS.reconfigure :exhale @15:22:15.03 SS.hold @15:22:24.06 Intake.select :close @15:22:24.22 SSV.dial :exhaust @15:22:24.99 Exhaust.select :open @15:22:25.10 SS.seek 0ml @15:22:33.81 Exhaust.select :close @15:22:33.93 SSV.dial CBV @15:22:33.97 CBV.dial SSV @15:22:34.73 Allowing 130s for 25.0ml of remaining 775.0ml @15:22:34.75 Intake.select :open @15:22:36.91 Intake @15.5psia, vacuum from 10.0 to 13.0psi @15:22:36.93 SS.reconfigure :inhaling 0.91ml/s @15:22:36.99 SS.seek 25ml @15:22:38.61 recoverPSRreagent08 @15:22:38.62 PTV.dial :puck @15:22:38.70 PBV.dial :waste1 @15:22:39.06 delay 30 seconds @15:23:07.01 Stroke completed -- waiting for pressure to equalize ... @15:23:07.03 SS.reconfigure :exhale @15:23:07.08 SS.hold @15:23:09.25 PBV.dial between PSV and waste1 @15:23:09.62 PSR.select :air @15:23:09.64 PSV.dial PSR @15:23:10.40 PS.reconfigure :fast @15:23:10.45 PS.seek 2.5ml @15:23:25.69 Intake.select :close @15:23:25.85 SSV.dial :exhaust @15:23:26.62 Exhaust.select :open @15:23:26.74 SS.seek 0ml @15:23:34.74 PSV.dial PTV @15:23:35.51 delay 5 seconds @15:23:35.73 Exhaust.select :close @15:23:35.85 SSV.dial CBV @15:23:35.88 CBV.dial SSV @15:23:36.64 Allowing 130s for 25.0ml of remaining 750.0ml @15:23:36.65 Intake.select :open @15:23:38.82 Intake @15.5psia, vacuum from 10.0 to 13.0psi @15:23:38.84 SS.reconfigure :inhaling 0.91ml/s @15:23:38.89 SS.seek 25ml @15:23:40.53 PSV.dial PSR @15:23:41.34 delay 5 seconds @15:23:46.36 PSV.dial PTV @15:23:47.24 delay 5 seconds @15:23:52.26 PSV.dial :air @15:23:53.03 PS.seek 0ml @15:24:02.29 Vacuum too high -- waiting up to 40 seconds @15:24:02.31 SS.reconfigure :exhale @15:24:02.36 SS.hold @15:24:13.67 Intake @15.3psia, vacuum from 9.8 to 12.8psi @15:24:13.69 SS.reconfigure :inhaling 0.73ml/s @15:24:13.74 SS.seek 25ml @15:24:16.71 washCycleDA #2 of 2 @15:24:16.72 PSR.select :air @15:24:16.74 PSV.dial PSR @15:24:18.07 Pulling 0.5ml of PSR.wash @15:24:18.08 PSR.select :wash @15:24:18.20 PS.reconfigure :default @15:24:18.25 PS.seek 0.5ml @15:24:21.53 Stroke completed -- waiting for pressure to equalize ... @15:24:21.54 SS.reconfigure :exhale @15:24:21.59 SS.hold @15:24:24.24 delay 5 seconds @15:24:29.26 PSR.select :air @15:24:29.44 PS.seek 2ml @15:24:34.63 Intake.select :close @15:24:34.79 SSV.dial :exhaust @15:24:35.56 Exhaust.select :open @15:24:35.68 SS.seek 0ml @15:24:44.73 Exhaust.select :close @15:24:44.85 SSV.dial CBV @15:24:44.87 CBV.dial SSV @15:24:45.52 PTV.dial :puck @15:24:45.60 PSV.dial PTV @15:24:45.67 Allowing 130s for 25.0ml of remaining 725.0ml @15:24:45.68 Intake.select :open @15:24:46.35 PBV.dial :waste1 @15:24:46.77 PS.seek 1.5ml @15:24:47.84 Intake @15.5psia, vacuum from 10.0 to 13.0psi @15:24:47.86 SS.reconfigure :inhaling 0.73ml/s @15:24:48.37 SS.seek 25ml @15:24:52.77 delay 3 seconds @15:24:55.79 PBV.dial between PSV and waste1 @15:24:56.15 PS.seek 0ml @15:25:12.27 delay 2:00 @15:25:21.75 Vacuum too high -- waiting up to 40 seconds @15:25:21.77 SS.reconfigure :exhale @15:25:21.83 SS.hold @15:25:34.70 Intake @15.2psia, vacuum from 9.7 to 12.7psi @15:25:34.72 SS.reconfigure :inhaling 0.59ml/s @15:25:34.77 SS.seek 25ml @15:25:38.01 Stroke completed -- waiting for pressure to equalize ... @15:25:38.03 SS.reconfigure :exhale @15:25:38.08 SS.hold @15:25:51.22 Intake.select :close @15:25:51.37 SSV.dial :exhaust @15:25:52.14 Exhaust.select :open @15:25:52.25 SS.seek 0ml @15:26:01.04 Exhaust.select :close @15:26:01.16 SSV.dial CBV @15:26:01.19 CBV.dial SSV @15:26:01.97 Allowing 130s for 25.0ml of remaining 700.0ml @15:26:01.98 Intake.select :open @15:26:04.15 Intake @15.5psia, vacuum from 10.0 to 13.0psi @15:26:04.17 SS.reconfigure :inhaling 0.59ml/s @15:26:04.22 SS.seek 25ml @15:26:45.94 Vacuum too high -- waiting up to 40 seconds @15:26:45.96 SS.reconfigure :exhale @15:26:46.01 SS.hold @15:27:00.99 Intake @15.3psia, vacuum from 9.8 to 12.8psi @15:27:01.01 SS.reconfigure :inhaling 0.47ml/s @15:27:01.06 SS.seek 25ml @15:27:05.29 Stroke completed -- waiting for pressure to equalize ... @15:27:05.30 SS.reconfigure :exhale @15:27:05.35 SS.hold @15:27:12.29 recoverPSRreagent08 @15:27:12.30 PTV.dial :puck @15:27:12.38 PBV.dial :waste1 @15:27:12.75 delay 30 seconds @15:27:18.09 Intake.select :close @15:27:18.25 SSV.dial :exhaust @15:27:19.03 Exhaust.select :open @15:27:19.14 SS.seek 0ml @15:27:27.90 Exhaust.select :close @15:27:28.01 SSV.dial CBV @15:27:28.04 CBV.dial SSV @15:27:28.82 Allowing 130s for 25.0ml of remaining 675.0ml @15:27:28.84 Intake.select :open @15:27:31.00 Intake @15.3psia, vacuum from 9.8 to 12.8psi @15:27:31.02 SS.reconfigure :inhaling 0.47ml/s @15:27:31.07 SS.seek 25ml @15:27:42.78 PBV.dial between PSV and waste1 @15:27:43.16 PSR.select :air @15:27:43.18 PSV.dial PSR @15:27:43.94 PS.reconfigure :fast @15:27:43.99 PS.seek 2.5ml @15:28:08.20 PSV.dial PTV @15:28:08.97 delay 5 seconds @15:28:13.99 PSV.dial PSR @15:28:14.75 delay 5 seconds @15:28:19.77 PSV.dial PTV @15:28:20.53 delay 5 seconds @15:28:25.55 PSV.dial :air @15:28:26.32 PS.seek 0ml @15:28:26.98 Stroke completed -- waiting for pressure to equalize ... @15:28:26.99 SS.reconfigure :exhale @15:28:27.04 SS.hold @15:28:49.81 getPMLReagent08 @15:28:49.84 PRV.dial :air @15:28:50.34 delay 5 seconds @15:28:55.36 PTV.dial :PRVmixing @15:28:56.67 delay 5 seconds @15:28:58.51 Intake.select :close @15:28:58.67 SSV.dial :exhaust @15:28:59.45 Exhaust.select :open @15:28:59.56 SS.seek 0ml @15:29:01.69 PRV.dial :ab2 @15:29:02.14 PTV.dial :PRVmixing @15:29:02.16 PSV.dial :mixloop @15:29:02.65 PS.reconfigure :default @15:29:02.70 PS.seek 0.3ml @15:29:06.58 delay 2 seconds @15:29:08.60 PRV.dial :air @15:29:08.76 Exhaust.select :close @15:29:08.87 SSV.dial CBV @15:29:08.89 CBV.dial SSV @15:29:09.11 PS.seek 0.55ml @15:29:09.68 Allowing 130s for 25.0ml of remaining 650.0ml @15:29:09.70 Intake.select :open @15:29:11.87 Intake @15.4psia, vacuum from 9.9 to 12.9psi @15:29:11.90 SS.reconfigure :inhaling 0.47ml/s @15:29:11.95 SS.seek 25ml @15:29:12.45 addPMLReagent08 @15:29:12.47 PSV.dial :air @15:29:12.94 PS.seek 2ml @15:29:28.76 PSV.dial :PML @15:29:29.23 PTV.dial :mixing @15:29:29.98 PBV.dial :waste1 @15:29:30.42 PS.seek 1.85ml @15:29:32.73 PBV.dial between PSV and waste1 @15:29:33.13 PS.seek 0ml @15:29:52.89 delay 2 seconds @15:29:54.91 delay 8:00 @15:29:59.28 Vacuum too high -- waiting up to 40 seconds @15:29:59.30 SS.reconfigure :exhale @15:29:59.35 SS.hold @15:30:18.37 Intake @15.7psia, vacuum from 10.0 to 13.0psi @15:30:18.39 SS.reconfigure :inhaling 0.38ml/s @15:30:18.44 SS.seek 25ml @15:30:28.68 Stroke completed -- waiting for pressure to equalize ... @15:30:28.69 SS.reconfigure :exhale @15:30:28.74 SS.hold @15:30:51.23 Intake.select :close @15:30:51.39 SSV.dial :exhaust @15:30:52.16 Exhaust.select :open @15:30:52.28 SS.seek 0ml @15:31:01.49 Exhaust.select :close @15:31:01.60 SSV.dial CBV @15:31:01.64 CBV.dial SSV @15:31:02.40 Allowing 130s for 25.0ml of remaining 625.0ml @15:31:02.41 Intake.select :open @15:31:04.57 Intake @15.2psia, vacuum from 9.7 to 12.7psi @15:31:04.59 SS.reconfigure :inhaling 0.38ml/s @15:31:04.64 SS.seek 25ml @15:32:14.22 Stroke completed -- waiting for pressure to equalize ... @15:32:14.23 SS.reconfigure :exhale @15:32:14.28 SS.hold @15:32:54.48 SS.reconfigure :default @15:32:54.53 SS.hold @15:32:54.60 Intake.select :close @15:32:54.77 ShallowSampler::Clogged in WCR -- Intake @15psia & Outlet @13psia won't equalize after 400.0ml @15:32:55.25 SSV.dial :exhaust @15:32:56.00 Exhaust.select :open @15:32:56.12 SS.reconfigure :exhale @15:32:56.18 SS.seek 0ml @15:33:04.99 Exhaust.select :close @15:33:05.10 Sampled 400.0ml @15:33:05.12 Drying puck before fixing for 5:00 @15:33:05.13 CBV.dial :waste2b @15:33:05.89 delay 1:00 @15:34:05.91 CSV.dial :air @15:34:06.38 CS.reconfigure :fast @15:34:06.43 CS.seek 10ml @15:34:31.14 Pumping wcr puck from tube 2 up to 35psi... @15:34:31.16 CBV.dial SSV @15:34:32.42 CBV.dial between CSV and waste2b @15:34:33.38 CSV.dial between air and CTV @15:34:33.71 CS.seek 7.283ml @15:34:40.83 CTV.dial CSV,avoiding: :air @15:34:40.86 CSV.dial CTV @15:34:41.69 CS.reconfigure :pressurize @15:34:41.74 CS.seek 0ml @15:35:00.88 CSV.dial :air @15:35:01.34 CS.reconfigure :fast @15:35:01.40 CS.seek 10ml @15:35:26.00 CSV.dial between air and CTV @15:35:26.33 CS.seek 4.424ml @15:35:40.08 CTV.dial CSV,avoiding: :air @15:35:40.10 CSV.dial CTV @15:35:40.50 CS.reconfigure :pressurize @15:35:40.55 CS.seek 0ml @15:35:52.48 CSV.dial :air @15:35:52.94 CS.reconfigure :fast @15:35:52.99 CS.seek 10ml @15:36:17.66 CSV.dial between air and CTV @15:36:17.98 CS.seek 4.535ml @15:36:31.67 CTV.dial CSV,avoiding: :air @15:36:31.70 CSV.dial CTV @15:36:32.06 CS.reconfigure :pressurize @15:36:32.12 CS.seek 0ml @15:36:44.23 CSV.dial :air @15:36:44.69 CS.reconfigure :fast @15:36:44.74 CS.seek 10ml @15:37:09.06 CSV.dial between air and CTV @15:37:09.39 CS.seek 4.549ml @15:37:22.64 CTV.dial CSV,avoiding: :air @15:37:22.66 CSV.dial CTV @15:37:23.14 CS.reconfigure :pressurize @15:37:23.19 CS.seek 0ml @15:37:35.32 CSV.dial :air @15:37:35.76 CS.reconfigure :default @15:37:35.81 CS.hold @15:37:35.94 ShallowSampler::Error in MAIN -- Puck.from 2, :wcr reached only 19.1 of 35psi after pumping in 40ml of gas @15:37:35.95 Waiting to Resume from Checkpoint "Pumping wcr puck from tube 2 up to 35psi..." @15:37:35.97 BadNews.email "Puck.from 2, :wcr reached only 19.1 of 35psi after pumping in 40ml of gas",Subject: "ShallowSampler::Error in MAIN" @15:37:40.27 upsync @15:37:54.93 recoverPRVreagent08 @15:37:54.95 PTV.dial :mixing @15:37:55.02 PBV.dial :waste1 @15:37:55.40 delay 30 seconds @15:38:25.43 PBV.dial between PSV and waste1 @15:38:25.79 PSV.dial :air @15:38:26.26 PS.reconfigure :fast @15:38:26.32 PS.seek 2.5ml @15:38:50.69 PSV.dial :mixloop @15:38:51.16 delay 2 seconds @15:38:53.18 PSV.dial :air @15:38:53.65 delay 2 seconds @15:38:55.66 PSV.dial :mixloop @15:38:56.15 delay 2 seconds @15:38:58.16 PSV.dial :air @15:38:58.76 PS.seek 0ml @15:39:22.32 washCycleDA #1 of 3 @15:39:22.79 PSR.select :air @15:39:22.84 PSV.dial PSR @15:39:24.28 Pulling 0.5ml of PSR.wash @15:39:24.30 PSR.select :wash @15:39:24.42 PS.reconfigure :default @15:39:24.47 PS.seek 0.5ml @15:39:30.38 delay 5 seconds @15:39:35.40 PSR.select :air @15:39:35.52 PS.seek 2ml @15:39:51.63 PTV.dial :puck @15:39:52.38 PSV.dial PTV @15:39:53.24 PBV.dial :waste1 @15:39:53.66 PS.seek 1.5ml @15:39:59.53 delay 3 seconds @15:40:02.55 PBV.dial between PSV and waste1 @15:40:02.91 PS.seek 0ml @15:40:19.25 delay 2:00 @15:42:19.28 recoverPSRreagent08 @15:42:19.29 PTV.dial :puck @15:42:19.36 PBV.dial :waste1 @15:42:19.74 delay 30 seconds @15:42:49.76 PBV.dial between PSV and waste1 @15:42:50.14 PSR.select :air @15:42:50.16 PSV.dial PSR @15:42:50.91 PS.reconfigure :fast @15:42:50.96 PS.seek 2.5ml @15:43:15.12 PSV.dial PTV @15:43:15.89 delay 5 seconds @15:43:20.91 PSV.dial PSR @15:43:21.67 delay 5 seconds @15:43:26.69 PSV.dial PTV @15:43:27.56 delay 5 seconds @15:43:32.58 PSV.dial :air @15:43:33.47 PS.seek 0ml @15:43:56.91 washCycleDA #2 of 3 @15:43:56.92 PSR.select :air @15:43:56.93 PSV.dial PSR @15:43:58.26 Pulling 0.5ml of PSR.wash @15:43:58.27 PSR.select :wash @15:43:58.39 PS.reconfigure :default @15:43:58.44 PS.seek 0.5ml @15:44:04.34 delay 5 seconds @15:44:09.36 PSR.select :air @15:44:09.48 PS.seek 2ml @15:44:25.62 PTV.dial :puck @15:44:25.70 PSV.dial PTV @15:44:26.47 PBV.dial :waste1 @15:44:26.90 PS.seek 1.5ml @15:44:32.81 delay 3 seconds @15:44:35.83 PBV.dial between PSV and waste1 @15:44:36.21 PS.seek 0ml @15:44:52.37 delay 2:00 @15:46:52.40 recoverPSRreagent08 @15:46:52.41 PTV.dial :puck @15:46:52.48 PBV.dial :waste1 @15:46:52.86 delay 30 seconds @15:47:22.88 PBV.dial between PSV and waste1 @15:47:23.26 PSR.select :air @15:47:23.28 PSV.dial PSR @15:47:24.03 PS.reconfigure :fast @15:47:24.08 PS.seek 2.5ml @15:47:48.24 PSV.dial PTV @15:47:49.59 delay 5 seconds @15:47:54.61 PSV.dial PSR @15:47:55.34 delay 5 seconds @15:48:00.36 PSV.dial PTV @15:48:01.10 delay 5 seconds @15:48:06.12 PSV.dial :air @15:48:06.89 PS.seek 0ml @15:48:30.18 washCycleDA #3 of 3 @15:48:30.19 PSR.select :air @15:48:30.21 PSV.dial PSR @15:48:31.54 Pulling 0.5ml of PSR.wash @15:48:31.55 PSR.select :wash @15:48:31.67 PS.reconfigure :default @15:48:31.72 PS.seek 0.5ml @15:48:37.62 delay 5 seconds @15:48:42.64 PSR.select :air @15:48:42.76 PS.seek 2ml @15:48:58.96 PTV.dial :puck @15:48:59.04 PSV.dial PTV @15:48:59.77 PBV.dial :waste1 @15:49:00.21 PS.seek 1.5ml @15:49:06.15 delay 3 seconds @15:49:09.17 PBV.dial between PSV and waste1 @15:49:09.54 PS.seek 0ml @15:49:25.90 delay 2:00 @15:51:25.92 recoverPSRreagent08 @15:51:25.93 PTV.dial :puck @15:51:26.00 PBV.dial :waste1 @15:51:26.38 delay 30 seconds @15:51:56.41 PBV.dial between PSV and waste1 @15:51:56.77 PSR.select :air @15:51:56.79 PSV.dial PSR @15:51:57.56 PS.reconfigure :fast @15:51:57.61 PS.seek 2.5ml @15:52:21.67 PSV.dial PTV @15:52:22.44 delay 5 seconds @15:52:27.46 PSV.dial PSR @15:52:28.21 delay 5 seconds @15:52:33.23 PSV.dial PTV @15:52:34.10 delay 5 seconds @15:52:39.12 PSV.dial :air @15:52:39.88 PS.seek 0ml @15:53:03.33 Gate.power :camera,:ON @15:53:03.35 Mixing DA Substrates PSR.select :air @15:53:03.37 PSV.dial PSR @15:53:04.77 Pulling 0.25ml of PSR.sub1 @15:53:04.80 PSR.select :sub1 @15:53:05.02 PS.reconfigure :default @15:53:05.15 PS.seek 0.25ml @15:53:08.53 delay 5 seconds @15:53:13.55 Pulling 0.25ml of PSR.sub2 @15:53:13.56 PSR.select :sub2 @15:53:13.68 PS.seek 0.5ml @15:53:17.08 delay 5 seconds @15:53:22.10 PSR.select :air @15:53:22.22 PS.seek 2ml @15:53:38.45 PTV.dial :puck @15:53:38.53 PSV.dial PTV @15:53:39.30 PBV.dial :waste1 @15:53:39.74 PS.seek 1.5ml @15:53:45.64 delay 3 seconds @15:53:48.66 PBV.dial between PSV and waste1 @15:53:49.51 PS.seek 0ml @15:54:05.64 delay 10 seconds @15:54:15.67 recoverPSRreagent08 @15:54:15.68 PTV.dial :puck @15:54:15.75 PBV.dial :waste1 @15:54:16.13 delay 30 seconds @15:54:46.15 PBV.dial between PSV and waste1 @15:54:46.53 PSR.select :air @15:54:46.55 PSV.dial PSR @15:54:47.32 PS.reconfigure :fast @15:54:47.37 PS.seek 2.5ml @15:55:11.76 PSV.dial PTV @15:55:12.53 delay 5 seconds @15:55:17.55 PSV.dial PSR @15:55:18.30 delay 5 seconds @15:55:23.32 PSV.dial PTV @15:55:24.08 delay 5 seconds @15:55:29.10 PSV.dial :air @15:55:29.99 PS.seek 0ml @15:55:54.05 Additional air clear @15:55:54.06 PSR.select :air @15:55:54.08 PSV.dial PSR @15:55:55.44 Pulling 2ml of PSR.air @15:55:55.45 PSR.select :air @15:55:55.47 PS.reconfigure :default @15:55:55.52 PS.seek 2ml @15:56:16.73 delay 5 seconds @15:56:21.75 PTV.dial :puck @15:56:21.84 PSV.dial PTV @15:56:22.73 PBV.dial :waste1 @15:56:23.17 PS.seek 1.5ml @15:56:29.04 delay 3 seconds @15:56:32.06 PBV.dial between PSV and waste1 @15:56:32.42 delay 1 second @15:56:33.44 PSV.dial :waste1 @15:56:34.51 PS.seek 0ml @15:56:50.76 Processing Puck Heater at 27.9C before imaging @15:56:50.77 PH.coast @15:56:50.88 Imaging da puck in Processing Clamp while storing it to tube 1 @15:56:51.06 SC.reconfigure :tight @15:56:51.11 SC.seek 1 @15:56:54.75 SC.configure :limp @15:56:54.98 Elbow.reconfigure :tight @15:56:55.03 Elbow.seek 1 @15:56:56.80 Forearm.reconfigure :tight @15:56:56.86 Forearm.seek 1 + 450 counts @15:56:57.95 SE.reconfigure :pick @15:56:58.01 SE.seek :beamBreak @15:57:00.34 SE.configure :default @15:57:00.50 Elbow.reconfigure :limp @15:57:00.98 Forearm.reconfigure :loose @15:57:01.09 Forearm.seek 1 @15:57:01.35 Elbow.hold @15:57:01.42 Elbow.reconfigure :tight @15:57:01.53 SE.seek 13.08 Pucks below Top Plate @15:57:02.10 Forearm.force -20 @15:57:02.15 Forearm.seek 1 + 312 counts @15:57:02.44 Forearm.reconfigure :tight @15:57:02.49 Forearm.seek 1 + 450 counts @15:57:02.70 SE.seek 14.08 Pucks below Top Plate @15:57:03.24 Forearm.reconfigure :loose @15:57:03.29 Forearm.seek :retract @15:57:04.22 Elbow.seek PC @15:57:06.70 Elbow.reconfigure :loose @15:57:06.80 Forearm.seek PC @15:57:07.73 Hand.close @15:57:08.34 PC.seek :open @15:57:13.17 Forearm.seek :retract @15:57:14.14 Elbow.reconfigure :tight @15:57:14.19 Elbow.seek 1 @15:57:16.80 Forearm.reconfigure :tight @15:57:16.85 Forearm.seek 1 + 450 counts @15:57:17.87 Hand.open @15:57:18.52 SE.seek 13.73 Pucks below Top Plate @15:57:18.79 SE.reconfigure :seal @15:57:18.84 SE.seek 12.77 Pucks below Top Plate @15:57:19.35 Lights, camera, ACTION! @15:57:19.39 PuckCamera: snap -bin=2,2 -auto /var/log/esp/da23sep2815h575ml.tif @15:57:38.90 Waiting >20 seconds for da to relinquish FlushPuck @15:57:42.99 SX HX9/16 (USB:04): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 20.296 seconds /var/log/esp/da23sep2815h575ml.tif: TIFF Upload Complete @15:57:43.02 PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da23sep2815h575ml15s.tif @15:57:58.63 SX HX9/16 (USB:04): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15 seconds /var/log/esp/midres/da23sep2815h575ml15s.tif: TIFF Upload Complete @15:57:58.66 PuckCamera: snap -bin=2,2 20.0 /var/log/esp/midres/da23sep2815h575ml20s.tif @15:58:19.31 SX HX9/16 (USB:04): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 20 seconds /var/log/esp/midres/da23sep2815h575ml20s.tif: TIFF Upload Complete @15:58:19.33 Gate.power :camera,:OFF @15:58:19.35 DAfans.email "20.296 sec auto-exposure",Subject: "da23sep2815h575ml images!" @15:58:19.77 Elbow.seek 1 @15:58:20.56 Forearm.seek 1 + 450 counts @15:58:20.64 SE.reconfigure :gently @15:58:20.70 SE.seek 13.48 Pucks below Top Plate @15:58:21.42 Elbow.reconfigure :limp @15:58:21.48 Forearm.reconfigure :loose @15:58:21.52 Forearm.seek 1 @15:58:21.82 Elbow.hold @15:58:21.91 Elbow.reconfigure :tight @15:58:21.96 SE.reconfigure :default @15:58:22.04 SE.seek 13.67 Pucks below Top Plate @15:58:22.41 Forearm.force -20 @15:58:22.45 Forearm.seek 1 + 309 counts @15:58:22.74 Forearm.reconfigure :tight @15:58:22.79 Forearm.seek 1 + 450 counts @15:58:23.17 SE.seek :down @15:58:24.45 Forearm.reconfigure :loose @15:58:24.56 Forearm.seek :retract @15:58:25.01 SE.stop @15:58:25.71 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @15:58:26.12 Elbow.seek FlushPuck::Garage @15:58:28.09 Elbow.reconfigure :loose @15:58:28.31 Forearm.seek :garage @15:58:29.39 Hand.close @15:58:30.39 Forearm.seek :retract @15:58:31.45 Elbow.reconfigure :tight @15:58:31.55 Elbow.seek PC @15:58:32.93 Elbow.reconfigure :loose @15:58:33.16 Forearm.reconfigure :tight @15:58:33.26 Forearm.seek PC @15:58:35.95 PC.seek :closed @15:58:40.78 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.63 @15:58:40.92 Hand.open @15:58:41.81 Forearm.reconfigure :loose @15:58:41.92 Forearm.seek :retract @15:58:42.93 PSR.select :air @15:58:42.99 PSV.dial PSR @15:58:43.52 Pulling 0.5ml of PSR.flush @15:58:43.54 PSR.select :flush @15:58:43.71 PS.seek 0.5ml @15:58:49.70 delay 5 seconds @15:58:53.35 upsync @15:58:54.72 PSR.select :air @15:58:54.85 PS.seek 2.5ml @15:59:16.17 delay 1 second @15:59:17.19 PRV.dial :waste2,avoiding: :ab2 @15:59:19.06 PTV.dial :PRVmixing @15:59:20.37 PSV.dial :mixloop @15:59:21.41 PS.seek :empty @15:59:47.73 PSR.select :air @15:59:47.75 PSV.dial PSR @15:59:48.79 Pulling 0.5ml of PSR.flush @15:59:48.80 PSR.select :flush @15:59:48.92 PS.seek 0.5ml @15:59:54.85 delay 5 seconds @15:59:59.87 PSR.select :air @15:59:59.99 PS.seek 2.5ml @16:00:21.26 delay 1 second @16:00:22.28 PBV.dial :waste1 @16:00:22.63 PTV.dial :mixing @16:00:23.38 PSV.dial :mixloop @16:00:24.43 PS.seek :empty @16:00:50.88 PSV.dial PSR @16:00:51.93 PSR.select :flush @16:00:52.05 PS.seek 1ml @16:01:03.19 PSR.select :air @16:01:03.31 PS.seek 2.5ml @16:01:19.47 PSV.dial PTV @16:01:20.35 PTV.dial :puck @16:01:21.09 PBV.dial :waste1 @16:01:21.62 PS.seek 0ml @16:01:48.01 PSV.dial PSR @16:01:48.74 PSR.select :flush @16:01:48.86 PS.seek 0.5ml @16:01:54.85 PSR.select :air @16:01:54.97 PS.seek 2.5ml @16:02:16.25 PSV.dial PTV @16:02:17.01 PBV.dial PSV @16:02:17.56 PS.seek 0ml @16:02:43.94 PSV.dial PBV @16:02:44.42 PS.seek 2.5ml @16:03:10.78 PSV.dial :waste1 @16:03:11.53 PS.seek 0ml @16:03:37.88 PSV.dial PSR @16:03:38.35 PSR.select :air @16:03:38.37 PS.seek 2.5ml @16:04:04.94 PSV.dial PTV @16:04:05.68 PBV.dial PSV @16:04:05.76 PS.seek 0ml @16:04:32.03 PSV.dial PBV @16:04:32.51 PS.seek 2.5ml @16:04:58.81 PSV.dial :waste1 @16:04:59.68 PS.seek 0ml @16:05:26.06 PSV.dial PSR @16:05:26.52 PSR.select :flush @16:05:26.64 PS.seek 1ml @16:05:37.66 PSR.select :air @16:05:37.78 PS.seek 2.5ml @16:05:54.00 PSV.dial PTV @16:05:54.86 PTV.dial :puck @16:05:54.88 PBV.dial :waste1 @16:05:55.43 PS.seek 0ml @16:06:21.75 PSV.dial PSR @16:06:22.50 PSR.select :flush @16:06:22.62 PS.seek 0.5ml @16:06:28.59 PSR.select :air @16:06:28.71 PS.seek 2.5ml @16:06:49.97 PSV.dial PTV @16:06:50.73 PBV.dial PSV @16:06:51.29 PS.seek 0ml @16:07:17.62 PSV.dial PBV @16:07:18.10 PS.seek 2.5ml @16:07:44.50 PSV.dial :waste1 @16:07:45.68 PS.seek 0ml @16:08:12.03 PSV.dial PSR @16:08:12.50 PSR.select :air @16:08:12.52 PS.seek 2.5ml @16:08:38.84 PSV.dial PTV @16:08:39.70 PBV.dial PSV @16:08:39.79 PS.seek 0ml @16:09:06.09 PSV.dial PBV @16:09:06.55 PS.seek 2.5ml @16:09:32.90 PSV.dial :waste1 @16:09:33.64 PS.seek 0ml @16:09:59.97 Garaging FlushPuck in Processing Clamp @16:10:00.04 Elbow.reconfigure :tight @16:10:00.09 Elbow.seek PC @16:10:00.21 Elbow.reconfigure :loose @16:10:00.32 Forearm.seek PC @16:10:01.26 Hand.close @16:10:01.83 PC.seek :open @16:10:06.58 Forearm.seek :retract @16:10:07.53 Elbow.reconfigure :tight @16:10:07.58 Elbow.seek :garage @16:10:08.93 Elbow.reconfigure :loose @16:10:09.03 Forearm.reconfigure :tight @16:10:09.08 Forearm.seek :garage @16:10:10.04 Hand.open @16:10:10.76 Forearm.reconfigure :loose @16:10:10.81 Forearm.seek :retract @18:05:47.11 Session Begun @18:05:50.13 -> CC Collection Clamp contains wcr puck from tube 2 @18:06:33.32 -> PC Processing Clamp open @18:08:09.08 -> CC.unloadPuck 1, :WCR @18:08:09.16 Puck::Warning in dave -- Unloading Puck.from 2, :wcr to Storage Carousel @18:08:09.18 Storing WCR puck in Collection Clamp to tube 1 @18:08:09.66 Elbow.reconfigure :tight @18:08:09.73 Elbow.seek 1 @18:08:11.58 Forearm.reconfigure :tight @18:08:12.10 Forearm.seek 1 + 450 counts @18:08:13.35 SE.reconfigure :pick @18:08:13.43 SE.seek :beamBreak @18:08:15.60 SE.configure :default @18:08:15.79 Elbow.reconfigure :limp @18:08:15.86 Forearm.reconfigure :loose @18:08:15.92 Forearm.seek 1 @18:08:16.23 Elbow.hold @18:08:16.32 Elbow.reconfigure :tight @18:08:16.44 SE.seek 14.06 Pucks below Top Plate @18:08:16.93 Forearm.force -20 @18:08:16.98 Forearm.seek 1 + 302 counts @18:08:17.41 Forearm.reconfigure :tight @18:08:17.48 Forearm.seek 1 + 450 counts @18:08:17.72 SE.seek 15.06 Pucks below Top Plate @18:08:18.27 Forearm.reconfigure :loose @18:08:18.34 Forearm.seek :retract @18:08:19.34 Elbow.seek CC @18:08:20.96 Elbow.reconfigure :loose @18:08:21.08 Forearm.seek CC @18:08:22.08 Hand.close @18:08:22.73 CC.seek :open @18:08:27.49 Forearm.seek :retract @18:08:28.53 Elbow.reconfigure :tight @18:08:28.60 Elbow.seek 1 @18:08:30.40 Elbow.reconfigure :limp @18:08:30.48 Forearm.seek 1 @18:08:31.46 Elbow.hold @18:08:31.56 Elbow.reconfigure :tight @18:08:31.62 Hand.open @18:08:32.35 Forearm.force -20 @18:08:32.41 Forearm.seek 1 + 319 counts @18:08:32.72 Forearm.reconfigure :tight @18:08:32.79 Forearm.seek 1 + 450 counts @18:08:33.06 SE.seek :down @18:08:34.58 Forearm.reconfigure :loose @18:08:34.72 Forearm.seek :retract @18:08:34.80 SE.stop @18:08:41.70 -> Puck.count @18:08:41.79 SE.seek :down @18:08:41.88 SE.stop @18:08:41.99 SC.reconfigure :tight @18:08:42.06 SC.seek 1 @18:08:42.15 SC.configure :limp @18:08:42.33 Elbow.seek 1 @18:08:42.54 Forearm.reconfigure :tight @18:08:42.62 Forearm.seek 1 + 450 counts @18:08:43.71 SE.reconfigure :place @18:08:43.79 SE.seek :beamBreak @18:08:46.21 SE.configure :default @18:08:46.34 SE.seek :down @18:08:47.47 Forearm.reconfigure :loose @18:08:47.55 Forearm.seek :retract @18:08:47.98 SE.stop @18:08:48.54 SC.reconfigure :tight @18:08:48.62 SC.seek 2 @18:08:52.20 SC.configure :limp @18:08:52.39 Elbow.seek 2 @18:08:52.60 Forearm.reconfigure :tight @18:08:52.67 Forearm.seek 2 + 450 counts @18:08:53.73 SE.reconfigure :place @18:08:53.81 SE.seek :beamBreak @18:08:56.04 SE.configure :default @18:08:56.17 SE.seek :down @18:08:57.30 Forearm.reconfigure :loose @18:08:57.37 Forearm.seek :retract @18:08:57.77 SE.stop @18:08:58.33 SC.reconfigure :tight @18:08:58.40 SC.seek 3 @18:09:01.81 SC.configure :limp @18:09:01.99 Elbow.seek 3 @18:09:02.53 Forearm.reconfigure :tight @18:09:02.60 Forearm.seek 3 + 450 counts @18:09:03.72 SE.reconfigure :place @18:09:03.80 SE.seek :beamBreak @18:09:04.93 SE.configure :default @18:09:05.06 SE.seek :down @18:09:05.50 SE.stop @18:09:06.20 Forearm.reconfigure :loose @18:09:06.27 Forearm.seek :retract @18:09:07.32 SC.reconfigure :tight @18:09:07.40 SC.seek 4 @18:09:10.95 SC.configure :limp @18:09:11.14 Elbow.seek 4 @18:09:11.63 Forearm.reconfigure :tight @18:09:11.70 Forearm.seek 4 + 450 counts @18:09:12.73 SE.reconfigure :place @18:09:12.81 SE.seek :beamBreak @18:09:13.98 SE.configure :default @18:09:14.11 SE.seek :down @18:09:14.58 SE.stop @18:09:15.25 Forearm.reconfigure :loose @18:09:15.32 Forearm.seek :retract @18:09:16.29 SC.reconfigure :tight @18:09:16.36 SC.seek 5 @18:09:19.89 SC.configure :limp @18:09:20.07 Elbow.seek 5 @18:09:20.29 Forearm.reconfigure :tight @18:09:20.35 Forearm.seek 5 + 450 counts @18:09:21.37 SE.reconfigure :place @18:09:21.45 SE.seek :beamBreak @18:09:22.62 SE.configure :default @18:09:22.75 SE.seek :down @18:09:23.25 SE.stop @18:09:23.88 Forearm.reconfigure :loose @18:09:23.96 Forearm.seek :retract @18:09:24.93 SC.reconfigure :tight @18:09:25.00 SC.seek 6 @18:09:28.53 SC.configure :limp @18:09:29.10 Elbow.seek 6 @18:09:29.38 Forearm.reconfigure :tight @18:09:29.45 Forearm.seek 6 + 450 counts @18:09:30.59 SE.reconfigure :place @18:09:30.67 SE.seek :beamBreak @18:09:31.87 SE.configure :default @18:09:32.00 SE.seek :down @18:09:32.42 SE.stop @18:09:33.14 Forearm.reconfigure :loose @18:09:33.21 Forearm.seek :retract @18:09:34.18 SC.reconfigure :tight @18:09:34.26 SC.seek 7 @18:09:37.80 SC.configure :limp @18:09:37.98 Elbow.seek 7 @18:09:38.54 Forearm.reconfigure :tight @18:09:38.61 Forearm.seek 7 + 450 counts @18:09:39.65 SE.reconfigure :place @18:09:39.73 SE.seek :beamBreak @18:09:41.05 SE.configure :default @18:09:41.18 SE.seek :down @18:09:41.80 SE.stop @18:09:42.32 Forearm.reconfigure :loose @18:09:42.39 Forearm.seek :retract {1 => 16, 2 => 17, 3 => 33, 4 => 33, 5 => 33, 6 => 33, 7 => 31} @18:11:25.21 -> PS.flush @18:11:25.23 Flushing Processing stage @18:11:25.32 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @18:11:25.53 Elbow.seek FlushPuck::Garage @18:11:27.41 Elbow.reconfigure :loose @18:11:27.53 Forearm.seek :garage @18:11:28.55 Hand.close @18:11:29.35 Forearm.seek :retract @18:11:30.36 Elbow.reconfigure :tight @18:11:30.43 Elbow.seek PC @18:11:31.70 Elbow.reconfigure :loose @18:11:31.83 Forearm.reconfigure :tight @18:11:31.90 Forearm.seek PC @18:11:33.65 PC.seek :closed @18:11:38.36 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.63 @18:11:38.45 Hand.open @18:11:39.16 Forearm.reconfigure :loose @18:11:39.23 Forearm.seek :retract @18:11:40.22 PSV.dial PSR @18:11:40.72 PSR.select :flush @18:11:40.86 PS.seek 1ml @18:11:51.88 PSR.select :air @18:11:52.02 PS.seek 2.5ml @18:12:08.16 PSV.dial PTV @18:12:09.04 PTV.dial :puck @18:12:09.05 PBV.dial :waste1 @18:12:09.64 PS.seek 0ml @18:12:36.04 PSV.dial PSR @18:12:36.91 PSR.select :flush @18:12:37.05 PS.seek 0.5ml @18:12:42.97 PSR.select :air @18:12:43.12 PS.seek 2.5ml @18:13:04.41 PSV.dial PTV @18:13:05.16 PBV.dial PSV @18:13:05.75 PS.seek 0ml @18:13:32.10 PSV.dial PBV @18:13:32.59 PS.seek 2.5ml @18:13:58.91 PSV.dial :waste1 @18:13:59.67 PS.seek 0ml @18:14:26.04 PSV.dial PSR @18:14:26.57 PSR.select :air @18:14:26.60 PS.seek 2.5ml @18:14:52.94 PSV.dial PTV @18:14:53.69 PBV.dial PSV @18:14:53.90 PS.seek 0ml @18:15:20.28 PSV.dial PBV @18:15:20.78 PS.seek 2.5ml @18:15:47.19 PSV.dial :waste1 @18:15:47.95 PS.seek 0ml @18:16:14.35 Garaging FlushPuck in Processing Clamp @18:16:14.44 Elbow.reconfigure :tight @18:16:14.51 Elbow.seek PC @18:16:15.13 Elbow.reconfigure :loose @18:16:15.33 Forearm.seek PC @18:16:16.30 Hand.close @18:16:16.97 PC.seek :open @18:16:21.72 Forearm.seek :retract @18:16:22.71 Elbow.reconfigure :tight @18:16:22.78 Elbow.seek :garage @18:16:24.21 Elbow.reconfigure :loose @18:16:24.34 Forearm.reconfigure :tight @18:16:24.41 Forearm.seek :garage @18:16:25.38 Hand.open @18:16:26.04 Forearm.reconfigure :loose @18:16:26.11 Forearm.seek :retract FlushPuck::Garage @18:18:27.24 -> exit @18:18:27.25 Session Ended + ESPwhich DA_WCR_100223.rb + fn=DA_WCR_100223.rb + IFS=: + set /home/esp/esp2/mission . + fullPath=/home/esp/esp2/mission/DA_WCR_100223.rb + [ -f /home/esp/esp2/mission/DA_WCR_100223.rb ] + realpath /home/esp/esp2/mission/DA_WCR_100223.rb + return 0 + scriptPath=/home/esp/esp2local/mission/DA_WCR_100223.rb + exec ruby /home/esp/esp2/lib/esp.rb /home/esp/esp2local/mission/DA_WCR_100223.rb @18:41:54.22UTC02-Oct-23
ESP2 v3.42 I2C Gateway v3.20 for core bus @18:41:56.84 Configuration for short puck jake 1/11/21 by brent@mbari.org /home/esp/esp2/type/shallow/jake/configure.rb @18:42:00.42 Polling Can every 10:00 @18:42:00.50 Cleanly exited at 18:37:42UTC02-Oct-23 @18:42:00.55 Loading /home/esp/esp2local/mission/DA_WCR_100223.rb mission @18:42:00.61 Preparing to sleep until + 00:00:05 @18:42:00.65 GoodNews.email "Starting DA_WCR_100223 w/pucks in tube 2\n{1 => 16,\n 2 => 17,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@18:42:00, 25% flow, 19.3C, 13% humidity, 14.7psia, 13.754V, 0.284A, 84.417Ah, 3.91W",Subject: "DA_WCR_100223 begun" @18:42:00.68
Asleep til + 00:00:05 @18:42:00.71 Continuing at 18:42 @18:42:00.95 Gate.power :core,:OFF @18:42:00.96 Gate.power :raw,:OFF @18:42:00.97 Shutdown @18:42:03.70 GoodNews.email "Then, in tube 2: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_100223.rb:14\n{1 => 16,\n 2 => 17,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@18:42:00, 25% flow, 19.3C, 13% humidity, 14.7psia, 13.749V, 0.290A, 84.417Ah, 3.99W",Subject: "Asleep until 18:42" @18:42:05.01 upsync @18:42:05.62
Powering core bus @18:42:07.81 upsync @18:42:11.02
Gate.power :raw,:ON @18:42:11.94 CS.configure :default @18:42:11.99 PS.configure :default @18:42:12.04 SS.configure :default @18:42:12.09 CC.configure :default @18:42:12.13 PC.configure :default @18:42:12.20 Forearm.configure :loose @18:42:12.25 Elbow.configure :loose @18:42:12.32 SC.configure :loose @18:42:12.37 SE.configure :default @18:42:12.41 CH.configure :default @18:42:12.45 PH.configure :default @18:42:12.49 All dwarf controllers running firmware version 3.85 @18:42:12.60 Valve.reset @18:42:12.87 Intake.select :close @18:42:12.89 Exhaust.select :close @18:42:12.92 CTV.dial :air @18:42:13.02 PSV.dial :air @18:42:13.13
Forearm.home.seek :retract @18:42:13.88 Hand.close @18:42:13.91
Elbow.reconfigure :loose @18:42:14.00 Elbow.jog 1500 @18:42:14.10 PS.home.seek 0ml @18:42:14.25 CSV.dial :waste1 @18:42:14.51
Elbow.reconfigure :loose @18:42:14.57 Elbow.home.seek :clear @18:42:14.98 SSV.dial :spit,avoiding: :exhaust @18:42:15.85 CS.reconfigure :fast @18:42:15.89 SS.home.seek 0ml @18:42:15.98 CS.home.seek 0ml @18:42:19.10
SE.home.seek :down @18:42:19.72 SC.reconfigure :tight @18:42:19.77 SC.home.seek :initial @18:42:22.42 PS.stop @18:42:48.16
SC.configure :limp @18:42:48.21 Forearm.seek :retract @18:42:48.28 Elbow.seek :hammer @18:42:49.29 Forearm.reconfigure :tight @18:42:49.34 Forearm.seek retracted @18:42:49.76 PC.home.seek :open @18:42:49.78
CC.home.seek :open @18:42:53.55 Phase [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] @18:42:53.57 Notice.email "At 18:42:53 began in tube 2: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_100223.rb:14\n{1 => 16,\n 2 => 17,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@18:42:53, 25% flow, 19.3C, 13% humidity, 14.7psia, 13.754V, 0.278A, 84.423Ah, 3.83W",Subject: "Started DA_WCR_100223 phase initialPurge; da [1000, 1000] #da and wsr are run simultanelously" @18:42:53.59
Starting initialPurge @18:42:53.61 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @18:42:53.67 Hand.open @18:42:54.43 Elbow.reconfigure :tight @18:42:54.47 Elbow.seek FlushPuck::Garage @18:42:56.43 Elbow.reconfigure :loose @18:42:56.61 Forearm.reconfigure :loose @18:42:56.66 Forearm.seek :garage @18:42:57.62 Hand.close @18:42:57.64 upsync @18:42:58.44
Forearm.seek :retract @18:42:59.40 Elbow.reconfigure :tight @18:42:59.45 Elbow.seek CC @18:43:02.41 Elbow.reconfigure :loose @18:43:02.51 Forearm.reconfigure :tight @18:43:02.56 Forearm.seek CC @18:43:03.88 CC.seek :closed @18:43:08.41 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.31 @18:43:08.47 Hand.open @18:43:09.12 Forearm.reconfigure :loose @18:43:09.16 Forearm.seek :retract @18:43:10.16 Flushing the Collection Valve::Manifold @18:43:10.29 CSV.dial CSR @18:43:11.57 Pulling 5ml of CSR.flush @18:43:11.58 CSR.select :flush @18:43:11.69 CS.reconfigure :default @18:43:11.74 CS.seek 5ml @18:43:25.72 delay 5 seconds @18:43:30.74 CSR.select :air @18:43:30.91 CS.reconfigure :fast @18:43:30.96 CS.seek 7.5ml @18:43:37.69 CSV.dial :waste @18:43:38.98 CS.reconfigure :default @18:43:39.02 CS.seek 0ml @18:43:59.04 Purging external Intake @18:43:59.16 CSV.dial :flush @18:43:59.62 CS.reconfigure :fast @18:43:59.66 CS.seek 10ml @18:44:24.22 delay 5 seconds @18:44:29.24 CSV.dial CBV @18:44:29.28
CBV.dial CSV @18:44:30.06 CTV.dial :intake @18:44:30.84 Intake.select :open @18:44:30.96 CS.seek 0ml @18:44:54.62 Intake.select :close @18:44:54.73 CTV.dial :air @18:44:55.52 Flushing upper manifold @18:44:55.64 CSV.dial :flush @18:44:56.38 CS.seek 5ml @18:45:09.22 delay 5 seconds @18:45:14.24 CSV.dial :air @18:45:14.86 CS.seek 7ml @18:45:20.44 CSV.dial CBV @18:45:20.48
CBV.dial CSV @18:45:21.47 CTV.dial :air @18:45:21.55 CS.seek 0ml @18:45:38.16 delay 7 seconds @18:45:45.18 CS.seek 3ml @18:45:53.19 CSV.dial :waste @18:45:53.65 CS.seek 0ml @18:46:01.36 CSV.dial :flush @18:46:01.80 CS.seek 3ml @18:46:09.84 delay 5 seconds @18:46:14.86 CSV.dial :air @18:46:15.38 CS.seek 5ml @18:46:21.09 CSV.dial :air @18:46:21.18 CS.seek 0ml @18:46:33.22 delay 7 seconds @18:46:40.25 CS.seek 3ml @18:46:48.22 CSV.dial :waste @18:46:48.96 CS.seek 0ml @18:46:56.59 Garaging FlushPuck in Collection Clamp @18:46:56.66 Elbow.reconfigure :tight @18:46:56.71 Elbow.seek CC @18:46:57.03 Elbow.reconfigure :loose @18:46:57.13 Forearm.seek CC @18:46:58.17 Hand.close @18:46:58.80 CC.seek :open @18:47:03.49 Forearm.seek :retract @18:47:04.52 Elbow.reconfigure :tight @18:47:04.57 Elbow.seek :garage @18:47:07.46 Elbow.reconfigure :loose @18:47:07.56 Forearm.reconfigure :tight @18:47:07.61 Forearm.seek :garage @18:47:08.62 Hand.open @18:47:09.23 Forearm.reconfigure :loose @18:47:09.28 Forearm.seek :retract @18:47:10.24 da sampling at most 1000ml, wcr at most 1000ml @18:47:10.28 Loading sh1 puck from tube 2 into Collection Clamp @18:47:10.30 DAfans.email "Started da in tube 2 at 18:47:10UTC02-Oct-23\n{1 => 16,\n 2 => 17,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@18:42:53, 25% flow, 19.3C, 13% humidity, 14.7psia, 13.754V, 0.278A, 84.423Ah, 3.83W",Subject: "da sampling at most 1000ml, wcr at most 1000ml" @18:47:10.57
SC.reconfigure :tight @18:47:10.63 SC.seek 2 @18:47:14.44 upsync @18:47:17.31
SC.configure :limp @18:47:17.54 Elbow.reconfigure :tight @18:47:17.59 Elbow.seek 2 @18:47:19.54 Forearm.reconfigure :tight @18:47:19.59 Forearm.seek 2 + 450 counts @18:47:20.61 SE.reconfigure :pick @18:47:20.72 SE.seek :beamBreak @18:47:22.74 SE.configure :default @18:47:22.90 Elbow.seek 2 @18:47:23.10 Forearm.seek 2 + 300 counts @18:47:23.38 Forearm.reconfigure :loose @18:47:23.43 Hand.close @18:47:24.06 SE.seek :down @18:47:24.64
Forearm.seek :retract @18:47:25.63 Elbow.seek CC @18:47:25.79 SE.stop @18:47:27.26
Elbow.reconfigure :loose @18:47:27.36 Forearm.reconfigure :tight @18:47:27.41 Forearm.seek CC @18:47:28.66 CC.seek :closed @18:47:33.19 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.22 @18:47:33.21 Hand.open @18:47:33.86 Forearm.reconfigure :loose @18:47:33.91 Forearm.seek :retract @18:47:34.93 collectSample 1000ml w/bubble point of 24psi @18:47:34.94 Priming 7ml @18:47:34.95 Intake.select :close @18:47:34.96 Exhaust.select :close @18:47:34.97 SS.hold @18:47:35.17 Calibrating pressure and temperature sensors @18:47:35.20 CTV.dial :air @18:47:35.28 SSV.dial :spit,avoiding: :exhaust @18:47:35.36 SS.reconfigure :fast @18:47:35.41 SS.seek 5ml @18:47:38.23 SSV.dial CBV @18:47:38.26
CBV.dial SSV @18:47:39.59 delay 3.8 seconds @18:47:43.52 Sensor limits: Intake max 115.8psia; Outlet max 108.9psia @18:47:43.53 SSV.dial :spit @18:47:44.86 SS.seek 0ml @18:47:47.88 SSV.dial CBV @18:47:49.18 CTV.dial :intake @18:47:49.97 SSV.dial CBV @18:47:50.01
CBV.dial SSV @18:47:50.13 Allowing 40s for 7.0ml of remaining 7.0ml @18:47:50.15 Intake.select :open @18:47:52.31 Intake @14.8psia, vacuum from 9.3 to 12.3psi @18:47:52.33 SS.reconfigure :inhaling 2.20ml/s @18:47:52.38 SS.seek 7ml @18:47:58.19 Stroke completed -- waiting for pressure to equalize ... @18:47:58.20 SS.reconfigure :exhale @18:47:58.25 SS.hold @18:48:01.99 Intake.select :close @18:48:02.14 SSV.dial :exhaust @18:48:02.89 Exhaust.select :open @18:48:03.00 SS.seek 0ml @18:48:07.21 Exhaust.select :close @18:48:07.33 Sampling 1000.0ml @18:48:07.34 Intake.select :close @18:48:07.35 Exhaust.select :close @18:48:07.36 SS.reconfigure :default @18:48:07.40 SS.hold @18:48:07.61 CTV.dial :intake @18:48:07.69 SSV.dial CBV @18:48:07.73
CBV.dial SSV @18:48:08.48 Allowing 130s for 25.0ml of remaining 1000.0ml @18:48:08.49 Intake.select :open @18:48:10.66 Intake @15.3psia, vacuum from 9.8 to 12.8psi @18:48:10.67 SS.reconfigure :inhaling 2.20ml/s @18:48:10.72 SS.seek 25ml @18:48:25.15 Stroke completed -- waiting for pressure to equalize ... @18:48:25.16 SS.reconfigure :exhale @18:48:25.20 SS.hold @18:48:37.86 Intake.select :close @18:48:38.04 SSV.dial :exhaust @18:48:38.79 Exhaust.select :open @18:48:38.90 SS.seek 0ml @18:48:47.95 Exhaust.select :close @18:48:48.06 SSV.dial CBV @18:48:48.09
CBV.dial SSV @18:48:48.85 Allowing 130s for 25.0ml of remaining 975.0ml @18:48:48.86 Intake.select :open @18:48:51.03 Intake @15.7psia, vacuum from 10.0 to 13.0psi @18:48:51.05 SS.reconfigure :inhaling 2.20ml/s @18:48:51.10 SS.seek 25ml @18:49:05.34 Stroke completed -- waiting for pressure to equalize ... @18:49:05.35 SS.reconfigure :exhale @18:49:05.39 SS.hold @18:49:18.06 Intake.select :close @18:49:18.22 SSV.dial :exhaust @18:49:18.96 Exhaust.select :open @18:49:19.08 SS.seek 0ml @18:49:27.85 Exhaust.select :close @18:49:27.96 SSV.dial CBV @18:49:28.00
CBV.dial SSV @18:49:28.73 Allowing 130s for 25.0ml of remaining 950.0ml @18:49:28.74 Intake.select :open @18:49:30.90 Intake @15.9psia, vacuum from 10.0 to 13.0psi @18:49:30.92 SS.reconfigure :inhaling 2.20ml/s @18:49:30.97 SS.seek 25ml @18:49:46.48 Stroke completed -- waiting for pressure to equalize ... @18:49:46.49 SS.reconfigure :exhale @18:49:46.54 SS.hold @18:49:59.21 Intake.select :close @18:49:59.36 SSV.dial :exhaust @18:50:00.12 Exhaust.select :open @18:50:00.23 SS.seek 0ml @18:50:08.99 Exhaust.select :close @18:50:09.10 SSV.dial CBV @18:50:09.12
CBV.dial SSV @18:50:09.91 Allowing 130s for 25.0ml of remaining 925.0ml @18:50:09.92 Intake.select :open @18:50:12.09 Intake @15.8psia, vacuum from 10.0 to 13.0psi @18:50:12.11 SS.reconfigure :inhaling 2.20ml/s @18:50:12.16 SS.seek 25ml @18:50:25.72 Vacuum too high -- waiting up to 40 seconds @18:50:25.73 SS.reconfigure :exhale @18:50:25.78 SS.hold @18:50:26.45 Intake @12.8psia, vacuum from 7.3 to 10.3psi @18:50:26.47 SS.reconfigure :inhaling 1.76ml/s @18:50:26.52 SS.seek 25ml @18:50:28.57 Stroke completed -- waiting for pressure to equalize ... @18:50:28.58 SS.reconfigure :exhale @18:50:28.62 SS.hold @18:50:40.53 Intake.select :close @18:50:40.69 SSV.dial :exhaust @18:50:41.43 Exhaust.select :open @18:50:41.54 SS.seek 0ml @18:50:50.55 Exhaust.select :close @18:50:50.67 SSV.dial CBV @18:50:50.69
CBV.dial SSV @18:50:51.46 Allowing 130s for 25.0ml of remaining 900.0ml @18:50:51.47 Intake.select :open @18:50:53.63 Intake @15.8psia, vacuum from 10.0 to 13.0psi @18:50:53.65 SS.reconfigure :inhaling 1.76ml/s @18:50:53.70 SS.seek 25ml @18:51:10.24 Stroke completed -- waiting for pressure to equalize ... @18:51:10.25 SS.reconfigure :exhale @18:51:10.29 SS.hold @18:51:19.61 Intake.select :close @18:51:19.77 SSV.dial :exhaust @18:51:20.54 Exhaust.select :open @18:51:20.65 SS.seek 0ml @18:51:29.43 Exhaust.select :close @18:51:29.54 SSV.dial CBV @18:51:29.57
CBV.dial SSV @18:51:30.33 Allowing 130s for 25.0ml of remaining 875.0ml @18:51:30.34 Intake.select :open @18:51:32.51 Intake @15.9psia, vacuum from 10.0 to 13.0psi @18:51:32.53 SS.reconfigure :inhaling 1.76ml/s @18:51:32.58 SS.seek 25ml @18:51:49.86 Stroke completed -- waiting for pressure to equalize ... @18:51:49.87 SS.reconfigure :exhale @18:51:49.92 SS.hold @18:52:00.97 Intake.select :close @18:52:01.12 SSV.dial :exhaust @18:52:01.88 Exhaust.select :open @18:52:01.99 SS.seek 0ml @18:52:10.75 Exhaust.select :close @18:52:10.86 SSV.dial CBV @18:52:10.89
CBV.dial SSV @18:52:12.10 Allowing 130s for 25.0ml of remaining 850.0ml @18:52:12.11 Intake.select :open @18:52:14.27 Intake @15.9psia, vacuum from 10.0 to 13.0psi @18:52:14.29 SS.reconfigure :inhaling 1.76ml/s @18:52:14.34 SS.seek 25ml @18:52:28.93 Vacuum too high -- waiting up to 40 seconds @18:52:28.95 SS.reconfigure :exhale @18:52:29.00 SS.hold @18:52:32.78 Intake @14.2psia, vacuum from 8.7 to 11.7psi @18:52:32.79 SS.reconfigure :inhaling 1.41ml/s @18:52:32.84 SS.seek 25ml @18:52:36.63 Stroke completed -- waiting for pressure to equalize ... @18:52:36.64 SS.reconfigure :exhale @18:52:36.68 SS.hold @18:52:42.30 Intake.select :close @18:52:42.45 SSV.dial :exhaust @18:52:43.19 Exhaust.select :open @18:52:43.30 SS.seek 0ml @18:52:52.27 Exhaust.select :close @18:52:52.38 SSV.dial CBV @18:52:52.41
CBV.dial SSV @18:52:53.18 Allowing 130s for 25.0ml of remaining 825.0ml @18:52:53.19 Intake.select :open @18:52:55.36 Intake @15.9psia, vacuum from 10.0 to 13.0psi @18:52:55.37 SS.reconfigure :inhaling 1.41ml/s @18:52:55.42 SS.seek 25ml @18:53:15.99 Stroke completed -- waiting for pressure to equalize ... @18:53:16.00 SS.reconfigure :exhale @18:53:16.04 SS.hold @18:53:25.80 Intake.select :close @18:53:25.95 SSV.dial :exhaust @18:53:26.73 Exhaust.select :open @18:53:26.84 SS.seek 0ml @18:53:35.88 Exhaust.select :close @18:53:35.99 SSV.dial CBV @18:53:36.01
CBV.dial SSV @18:53:36.80 Allowing 130s for 25.0ml of remaining 800.0ml @18:53:36.81 Intake.select :open @18:53:38.97 Intake @15.5psia, vacuum from 10.0 to 13.0psi @18:53:38.99 SS.reconfigure :inhaling 1.41ml/s @18:53:39.04 SS.seek 25ml @18:53:58.83 Stroke completed -- waiting for pressure to equalize ... @18:53:58.84 SS.reconfigure :exhale @18:53:58.88 SS.hold @18:54:11.62 Intake.select :close @18:54:11.77 SSV.dial :exhaust @18:54:12.53 Exhaust.select :open @18:54:12.65 SS.seek 0ml @18:54:21.81 Exhaust.select :close @18:54:21.92 SSV.dial CBV @18:54:21.95
CBV.dial SSV @18:54:22.74 Allowing 130s for 25.0ml of remaining 775.0ml @18:54:22.75 Intake.select :open @18:54:24.91 Intake @15.7psia, vacuum from 10.0 to 13.0psi @18:54:24.93 SS.reconfigure :inhaling 1.41ml/s @18:54:24.98 SS.seek 25ml @18:54:44.67 Stroke completed -- waiting for pressure to equalize ... @18:54:44.68 SS.reconfigure :exhale @18:54:44.73 SS.hold @18:54:59.10 Intake.select :close @18:54:59.26 SSV.dial :exhaust @18:55:00.00 Exhaust.select :open @18:55:00.12 SS.seek 0ml @18:55:09.14 Exhaust.select :close @18:55:09.25 SSV.dial CBV @18:55:09.27
CBV.dial SSV @18:55:10.06 Allowing 130s for 25.0ml of remaining 750.0ml @18:55:10.08 Intake.select :open @18:55:12.24 Intake @15.7psia, vacuum from 10.0 to 13.0psi @18:55:12.26 SS.reconfigure :inhaling 1.41ml/s @18:55:12.31 SS.seek 25ml @18:55:29.93 Vacuum too high -- waiting up to 40 seconds @18:55:29.94 SS.reconfigure :exhale @18:55:29.99 SS.hold @18:55:37.64 SS.reconfigure :inhaling 1.13ml/s @18:55:37.69 SS.seek 25ml @18:55:41.22 Stroke completed -- waiting for pressure to equalize ... @18:55:41.23 SS.reconfigure :exhale @18:55:41.28 SS.hold @18:55:47.94 Intake.select :close @18:55:48.09 SSV.dial :exhaust @18:55:48.84 Exhaust.select :open @18:55:48.96 SS.seek 0ml @18:55:57.98 Exhaust.select :close @18:55:58.09 SSV.dial CBV @18:55:58.12
CBV.dial SSV @18:55:58.89 Allowing 130s for 25.0ml of remaining 725.0ml @18:55:58.90 Intake.select :open @18:56:01.06 Intake @15.5psia, vacuum from 10.0 to 13.0psi @18:56:01.08 SS.reconfigure :inhaling 1.13ml/s @18:56:01.13 SS.seek 25ml @18:56:25.17 Stroke completed -- waiting for pressure to equalize ... @18:56:25.18 SS.reconfigure :exhale @18:56:25.23 SS.hold @18:56:39.67 Intake.select :close @18:56:39.82 SSV.dial :exhaust @18:56:40.58 Exhaust.select :open @18:56:40.69 SS.seek 0ml @18:56:49.44 Exhaust.select :close @18:56:49.55 SSV.dial CBV @18:56:49.57
CBV.dial SSV @18:56:50.36 Allowing 130s for 25.0ml of remaining 700.0ml @18:56:50.37 Intake.select :open @18:56:52.53 Intake @15.6psia, vacuum from 10.0 to 13.0psi @18:56:52.55 SS.reconfigure :inhaling 1.13ml/s @18:56:52.60 SS.seek 25ml @18:57:13.52 Vacuum too high -- waiting up to 40 seconds @18:57:13.53 SS.reconfigure :exhale @18:57:13.59 SS.hold @18:57:21.16 Intake @15.1psia, vacuum from 9.6 to 12.6psi @18:57:21.17 SS.reconfigure :inhaling 0.91ml/s @18:57:21.22 SS.seek 25ml @18:57:27.03 Stroke completed -- waiting for pressure to equalize ... @18:57:27.04 SS.reconfigure :exhale @18:57:27.08 SS.hold @18:57:36.85 Intake.select :close @18:57:37.00 SSV.dial :exhaust @18:57:37.76 Exhaust.select :open @18:57:37.87 SS.seek 0ml @18:57:46.90 Exhaust.select :close @18:57:47.01 SSV.dial CBV @18:57:47.04
CBV.dial SSV @18:57:47.81 Allowing 130s for 25.0ml of remaining 675.0ml @18:57:47.82 Intake.select :open @18:57:49.98 Intake @15.5psia, vacuum from 10.0 to 13.0psi @18:57:50.00 SS.reconfigure :inhaling 0.91ml/s @18:57:50.05 SS.seek 25ml @18:58:19.71 Stroke completed -- waiting for pressure to equalize ... @18:58:19.72 SS.reconfigure :exhale @18:58:19.77 SS.hold @18:58:35.99 Intake.select :close @18:58:36.14 SSV.dial :exhaust @18:58:36.89 Exhaust.select :open @18:58:37.00 SS.seek 0ml @18:58:45.71 Exhaust.select :close @18:58:45.83 SSV.dial CBV @18:58:45.85
CBV.dial SSV @18:58:46.62 Allowing 130s for 25.0ml of remaining 650.0ml @18:58:46.63 Intake.select :open @18:58:48.80 Intake @15.2psia, vacuum from 9.7 to 12.7psi @18:58:48.82 SS.reconfigure :inhaling 0.91ml/s @18:58:48.87 SS.seek 25ml @18:59:18.33 Vacuum too high -- waiting up to 40 seconds @18:59:18.34 SS.reconfigure :exhale @18:59:18.40 SS.hold @18:59:29.66 Intake @15.0psia, vacuum from 9.5 to 12.5psi @18:59:29.67 SS.reconfigure :inhaling 0.73ml/s @18:59:29.72 SS.seek 25ml @18:59:30.73 Stroke completed -- waiting for pressure to equalize ... @18:59:30.74 SS.reconfigure :exhale @18:59:30.78 SS.hold @18:59:38.68 Intake.select :close @18:59:38.84 SSV.dial :exhaust @18:59:39.59 Exhaust.select :open @18:59:39.70 SS.seek 0ml @18:59:48.49 Exhaust.select :close @18:59:48.61 SSV.dial CBV @18:59:48.63
CBV.dial SSV @18:59:49.40 Allowing 130s for 25.0ml of remaining 625.0ml @18:59:49.42 Intake.select :open @18:59:51.58 Intake @15.5psia, vacuum from 10.0 to 13.0psi @18:59:51.60 SS.reconfigure :inhaling 0.73ml/s @18:59:51.65 SS.seek 25ml @19:00:28.07 Stroke completed -- waiting for pressure to equalize ... @19:00:28.08 SS.reconfigure :exhale @19:00:28.13 SS.hold @19:00:48.59 Intake.select :close @19:00:48.75 SSV.dial :exhaust @19:00:49.51 Exhaust.select :open @19:00:49.65 SS.seek 0ml @19:00:58.40 Exhaust.select :close @19:00:58.51 SSV.dial CBV @19:00:58.55
CBV.dial SSV @19:00:59.27 Allowing 130s for 25.0ml of remaining 600.0ml @19:00:59.29 Intake.select :open @19:01:01.45 Intake @15.5psia, vacuum from 10.0 to 13.0psi @19:01:01.47 SS.reconfigure :inhaling 0.73ml/s @19:01:01.52 SS.seek 25ml @19:01:35.52 Vacuum too high -- waiting up to 40 seconds @19:01:35.53 SS.reconfigure :exhale @19:01:35.58 SS.hold @19:01:50.56 Intake @15.1psia, vacuum from 9.6 to 12.6psi @19:01:50.58 SS.reconfigure :inhaling 0.59ml/s @19:01:50.63 SS.seek 25ml @19:01:53.85 Stroke completed -- waiting for pressure to equalize ... @19:01:53.86 SS.reconfigure :exhale @19:01:53.91 SS.hold @19:02:03.58 Intake.select :close @19:02:03.73 SSV.dial :exhaust @19:02:04.49 Exhaust.select :open @19:02:04.61 SS.seek 0ml @19:02:13.41 Exhaust.select :close @19:02:13.52 SSV.dial CBV @19:02:13.55
CBV.dial SSV @19:02:14.30 Allowing 130s for 25.0ml of remaining 575.0ml @19:02:14.32 Intake.select :open @19:02:16.48 Intake @15.2psia, vacuum from 9.7 to 12.7psi @19:02:16.50 SS.reconfigure :inhaling 0.59ml/s @19:02:16.55 SS.seek 25ml @19:03:01.50 Stroke completed -- waiting for pressure to equalize ... @19:03:01.52 SS.reconfigure :exhale @19:03:01.56 SS.hold @19:03:26.19 Intake.select :close @19:03:26.35 SSV.dial :exhaust @19:03:27.10 Exhaust.select :open @19:03:27.21 SS.seek 0ml @19:03:36.24 Exhaust.select :close @19:03:36.35 SSV.dial CBV @19:03:36.38
CBV.dial SSV @19:03:37.15 Allowing 130s for 25.0ml of remaining 550.0ml @19:03:37.16 Intake.select :open @19:03:39.32 Intake @15.1psia, vacuum from 9.6 to 12.6psi @19:03:39.34 SS.reconfigure :inhaling 0.59ml/s @19:03:39.39 SS.seek 25ml @19:04:18.86 Vacuum too high -- waiting up to 40 seconds @19:04:18.87 SS.reconfigure :exhale @19:04:18.92 SS.hold @19:04:32.15 Intake @14.7psia, vacuum from 9.2 to 12.2psi @19:04:32.16 SS.reconfigure :inhaling 0.47ml/s @19:04:32.21 SS.seek 25ml @19:04:38.67 Stroke completed -- waiting for pressure to equalize ... @19:04:38.69 SS.reconfigure :exhale @19:04:38.73 SS.hold @19:04:57.08 Intake.select :close @19:04:57.23 SSV.dial :exhaust @19:04:57.99 Exhaust.select :open @19:04:58.10 SS.seek 0ml @19:05:07.13 Exhaust.select :close @19:05:07.24 SSV.dial CBV @19:05:07.28
CBV.dial SSV @19:05:08.00 Allowing 130s for 25.0ml of remaining 525.0ml @19:05:08.02 Intake.select :open @19:05:10.18 Intake @15.0psia, vacuum from 9.5 to 12.5psi @19:05:10.20 SS.reconfigure :inhaling 0.47ml/s @19:05:10.25 SS.seek 25ml @19:06:05.74 Stroke completed -- waiting for pressure to equalize ... @19:06:05.75 SS.reconfigure :exhale @19:06:05.79 SS.hold @19:06:34.38 Intake.select :close @19:06:34.53 SSV.dial :exhaust @19:06:35.28 Exhaust.select :open @19:06:35.40 SS.seek 0ml @19:06:44.17 Exhaust.select :close @19:06:44.28 SSV.dial CBV @19:06:44.31
CBV.dial SSV @19:06:45.08 Allowing 130s for 25.0ml of remaining 500.0ml @19:06:45.09 Intake.select :open @19:06:47.25 Intake @15.0psia, vacuum from 9.5 to 12.5psi @19:06:47.27 SS.reconfigure :inhaling 0.47ml/s @19:06:47.32 SS.seek 25ml @19:07:43.45 Stroke completed -- waiting for pressure to equalize ... @19:07:43.46 SS.reconfigure :exhale @19:07:43.51 SS.hold @19:08:18.57 Intake.select :close @19:08:18.73 SSV.dial :exhaust @19:08:19.48 Exhaust.select :open @19:08:19.60 SS.seek 0ml @19:08:28.39 Exhaust.select :close @19:08:28.50 SSV.dial CBV @19:08:28.52
CBV.dial SSV @19:08:29.30 Allowing 130s for 25.0ml of remaining 475.0ml @19:08:29.31 Intake.select :open @19:08:31.47 Intake @15.0psia, vacuum from 9.5 to 12.5psi @19:08:31.49 SS.reconfigure :inhaling 0.47ml/s @19:08:31.54 SS.seek 25ml @19:09:18.44 Vacuum too high -- waiting up to 40 seconds @19:09:18.46 SS.reconfigure :exhale @19:09:18.51 SS.hold @19:09:39.38 Intake @15.6psia, vacuum from 10.0 to 13.0psi @19:09:39.39 SS.reconfigure :inhaling 0.38ml/s @19:09:39.44 SS.seek 25ml @19:09:52.06 Stroke completed -- waiting for pressure to equalize ... @19:09:52.07 SS.reconfigure :exhale @19:09:52.11 SS.hold @19:10:12.52 Intake.select :close @19:10:13.11 SSV.dial :exhaust @19:10:13.86 Exhaust.select :open @19:10:13.97 SS.seek 0ml @19:10:22.98 Exhaust.select :close @19:10:23.09 SSV.dial CBV @19:10:23.12
CBV.dial SSV @19:10:23.89 Allowing 130s for 25.0ml of remaining 450.0ml @19:10:23.90 Intake.select :open @19:10:26.07 Intake @15.1psia, vacuum from 9.6 to 12.6psi @19:10:26.08 SS.reconfigure :inhaling 0.38ml/s @19:10:26.13 SS.seek 25ml @19:11:37.20 Stroke completed -- waiting for pressure to equalize ... @19:11:37.21 SS.reconfigure :exhale @19:11:37.25 SS.hold @19:12:12.12 Intake.select :close @19:12:12.27 SSV.dial :exhaust @19:12:13.03 Exhaust.select :open @19:12:13.14 SS.seek 0ml @19:12:21.96 Exhaust.select :close @19:12:22.07 SSV.dial CBV @19:12:22.10
CBV.dial SSV @19:12:22.87 Allowing 130s for 25.0ml of remaining 425.0ml @19:12:22.88 Intake.select :open @19:12:25.04 Intake @15.0psia, vacuum from 9.5 to 12.5psi @19:12:25.06 SS.reconfigure :inhaling 0.38ml/s @19:12:25.11 SS.seek 25ml @19:13:34.81 Vacuum too high -- waiting up to 40 seconds @19:13:34.83 SS.reconfigure :exhale @19:13:34.93 SS.hold @19:13:58.46 Intake @14.9psia, vacuum from 9.4 to 12.4psi @19:13:58.48 SS.reconfigure :inhaling 0.31ml/s @19:13:58.53 SS.seek 25ml @19:14:01.38 Stroke completed -- waiting for pressure to equalize ... @19:14:01.39 SS.reconfigure :exhale @19:14:01.44 SS.hold @19:14:19.84 Intake.select :close @19:14:20.00 SSV.dial :exhaust @19:14:20.74 Exhaust.select :open @19:14:20.86 SS.seek 0ml @19:14:29.66 Exhaust.select :close @19:14:29.77 SSV.dial CBV @19:14:29.80
CBV.dial SSV @19:14:30.55 Allowing 130s for 25.0ml of remaining 400.0ml @19:14:30.57 Intake.select :open @19:14:32.73 Intake @15.0psia, vacuum from 9.5 to 12.5psi @19:14:33.10 SS.reconfigure :inhaling 0.31ml/s @19:14:33.22 SS.seek 25ml @19:16:06.60 Stroke completed -- waiting for pressure to equalize ... @19:16:06.61 SS.reconfigure :exhale @19:16:06.66 SS.hold @19:16:40.58 SS.reconfigure :default @19:16:40.63 SS.hold @19:16:40.69 Intake.select :close @19:16:40.85 ShallowSampler::Clogged in MAIN -- Filtering too Slowly after 625.0ml @19:16:40.86 SSV.dial :exhaust @19:16:42.05 Exhaust.select :open @19:16:42.16 SS.reconfigure :exhale @19:16:42.21 SS.seek 0ml @19:16:51.29 Exhaust.select :close @19:16:51.40 Sampled 625.0ml @19:16:51.41 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @19:16:51.42 Intake.select :close @19:16:51.49 CSV.dial :waste @19:16:51.64 SSV.dial CBV,avoiding: :exhaust @19:16:51.68 CS.seek 0ml @19:16:51.71
CBV.dial SSV @19:16:51.86 CSV.dial :air @19:16:52.60 CS.seek 10ml @19:16:53.60
CTV.dial :air @19:16:54.39 delay 15 seconds @19:17:09.41 SSV.dial :spit,avoiding: :exhaust @19:17:10.73 CTV.dial CSV,avoiding: :intake @19:17:10.77
SS.seek 0ml @19:17:10.89 SSV.dial CBV,avoiding: :exhaust @19:17:17.10 SSV.dial between CBV and plug @19:17:17.59 CBV.dial between SSV and waste2 @19:17:17.99 CTV.dial CSV,avoiding: :air @19:17:18.02
CSV.dial CTV @19:17:18.50 CS.reconfigure :pressurize @19:17:18.55 CS.seek 0ml @19:17:36.54 BubblePoint: Intake @24.2psia, Outlet @0.7psia @19:17:36.55 CBV.dial SSV @19:17:36.88 SS.reconfigure :default @19:17:36.93 SS.seek 10ml @19:17:42.50 delay 15 seconds @19:17:57.63 Evacuated: Intake @23.8psia, Outlet @24.1psia @19:17:57.65 SSV.dial :spit,avoiding: :exhaust @19:17:58.68 SS.reconfigure :exhale @19:17:58.73 SS.seek 0ml @19:18:03.69 SS.reconfigure :fast @19:18:03.73 SS.seek 5ml @19:18:06.73 SSV.dial CBV,avoiding: :exhaust @19:18:06.76
CBV.dial SSV @19:18:08.10 CTV.dial :air,avoiding: :intake @19:18:09.44 CSV.dial CTV @19:18:09.49
SS.seek 10ml @19:18:10.60 CSV.dial :air @19:18:11.05 CS.reconfigure :fast @19:18:11.10 CS.seek 0ml @19:18:12.12
SSV.dial :spit,avoiding: :exhaust @19:18:13.45 SS.reconfigure :exhale @19:18:13.92 SS.seek 0ml @19:18:19.15 CS.stop @19:18:19.22
SS.reconfigure :fast @19:18:19.27 SS.seek 5ml @19:18:22.02 SSV.dial CBV,avoiding: :exhaust @19:18:22.05
CBV.dial SSV @19:18:24.42 SSV.dial :spit,avoiding: :exhaust @19:18:25.74 SS.reconfigure :default @19:18:25.79 SS.seek 0ml @19:18:29.20 SS.stop @19:18:29.24 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @19:18:29.25 Intake.select :close @19:18:29.32 CSV.dial :waste @19:18:29.44 SSV.dial CBV,avoiding: :exhaust @19:18:29.48
CBV.dial SSV @19:18:30.10 CS.seek 0ml @19:18:30.16 CSV.dial :air @19:18:30.80
CTV.dial :air @19:18:30.91 CS.seek 10ml @19:18:30.99
delay 15 seconds @19:18:46.00 SSV.dial :spit,avoiding: :exhaust @19:18:47.32 CTV.dial CSV,avoiding: :intake @19:18:47.36
SS.reconfigure :exhale @19:18:47.45 SS.seek 0ml @19:18:47.52 SSV.dial CBV,avoiding: :exhaust @19:18:55.47 SSV.dial between CBV and plug @19:18:55.96 CBV.dial between SSV and waste2 @19:18:56.35 CSV.dial between air and CTV @19:18:56.66 CS.seek 7.274ml @19:19:03.60 CTV.dial CSV,avoiding: :air @19:19:03.63
CSV.dial CTV @19:19:03.97 CS.reconfigure :pressurize @19:19:04.02 CS.seek 0ml @19:19:18.41 BubblePoint: Intake @24.1psia, Outlet @4.7psia CBV.dial SSV @19:19:18.72 SS.reconfigure :default @19:19:18.77 SS.seek 10ml @19:19:24.21 delay 15 seconds @19:19:39.34 Evacuated: Intake @23.7psia, Outlet @24.2psia @19:19:39.35 SSV.dial :spit,avoiding: :exhaust @19:19:40.40 SS.reconfigure :exhale @19:19:40.45 SS.seek 0ml @19:19:45.64 SS.reconfigure :fast @19:19:45.68 SS.seek 5ml @19:19:48.35 SSV.dial CBV,avoiding: :exhaust @19:19:48.81
CBV.dial SSV @19:19:50.14 CTV.dial :air,avoiding: :intake @19:19:51.48 CSV.dial CTV @19:19:51.52
SS.seek 10ml @19:19:52.64 CSV.dial :air @19:19:53.10 CS.reconfigure :fast @19:19:53.15 CS.seek 0ml @19:19:54.00
SSV.dial :spit,avoiding: :exhaust @19:19:55.33 SS.reconfigure :exhale @19:19:55.38 SS.seek 0ml @19:19:58.28 CS.stop @19:20:00.31
SS.reconfigure :fast @19:20:00.36 SS.seek 5ml @19:20:03.13 SSV.dial CBV,avoiding: :exhaust @19:20:03.16
CBV.dial SSV @19:20:05.48 SSV.dial :spit,avoiding: :exhaust @19:20:06.80 SS.reconfigure :default @19:20:06.85 SS.seek 0ml @19:20:10.09 SS.stop @19:20:10.14 Lysing: Adding 2.0ml AqMeOH @19:20:10.15 CBV.dial between CSV and waste1,avoiding: :DBV @19:20:11.62 CTV.dial CSV @19:20:12.93 CSV.dial CSR @19:20:13.72 Pulling 1ml of CSR.AqMeOH @19:20:13.73 CSR.select :AqMeOH @19:20:13.84 CS.reconfigure :default @19:20:13.89 CS.seek 1ml @19:20:17.37 delay 5 seconds @19:20:22.39 CSR.select :air @19:20:22.56 CS.seek 6.001ml @19:20:36.12 delay 2 seconds @19:20:38.14 CSV.dial :waste2 @19:20:38.58 CS.reconfigure :fast @19:20:38.63 CS.seek 0ml @19:20:52.99 CSV.dial CSR @19:20:53.45 Pulling 2ml of CSR.AqMeOH @19:20:53.46 CSR.select :AqMeOH @19:20:53.57 CS.reconfigure :default @19:20:53.62 CS.seek 2ml @19:20:59.62 delay 5 seconds @19:21:04.64 CSR.select :air @19:21:04.81 CS.seek 5.001ml @19:21:13.37 delay 2 seconds @19:21:15.39 CSV.dial CTV @19:21:15.83 CS.seek 1ml @19:21:27.32 Before lysing Collection Puck Heater at 18.1C/34.4psia @19:21:27.35 Heating Puck.from 2, :sh1 in Collection Clamp to 70C for 10:00 @19:21:27.36 CH.seek 70C,8:00 @19:23:32.71 CH.hold 10:00,70C @19:23:33.36 Begun lysing Collection Puck Heater at 68C/40.2psia @19:33:32.92 Ended lysing Collection Puck Heater at 70C/39.6psia @19:33:32.94 CS.seek 0ml @19:33:36.52 CTV.dial between spare and air,avoiding: :intake @19:33:37.58 CSV.dial :air @19:33:38.03 CS.seek 5ml @19:33:51.82 CSV.dial CTV @19:33:52.28 delay 2 seconds @19:33:54.30 CSV.dial :air @19:33:54.75 delay 2 seconds @19:33:56.76 CSV.dial CTV @19:33:57.22 delay 2 seconds @19:33:59.23 CSV.dial :air @19:33:59.69 delay 2 seconds @19:34:01.71 CS.seek 0ml @19:34:15.38 Puck @101.1psia after final lysis pressurization @19:34:15.39 Recovering Lysate into Collection syringe @19:34:15.40 CSV.dial CBV @19:34:16.40 CBV.dial CSV @19:34:16.79 CS.seek 5ml @19:34:30.45 delay 5 seconds @19:34:35.47 CTV.dial :air @19:34:35.99 CS.seek 7ml @19:34:42.04 delay 5 seconds @19:34:47.06 CSV.dial :air2 @19:34:47.50 CS.seek 10ml @19:34:55.95 CSV.dial between air and CTV,avoiding: CBV @19:34:57.09 Lysing Complete @19:34:57.16 Storing sh1 puck in Collection Clamp to tube 1 @19:34:57.34 SC.reconfigure :tight @19:34:57.39 SC.seek 1 @19:35:00.92 SC.configure :limp @19:35:01.15 Elbow.reconfigure :tight @19:35:01.19 Elbow.seek 1 @19:35:03.00 Forearm.reconfigure :tight @19:35:03.05 Forearm.seek 1 + 450 counts @19:35:04.10 SE.reconfigure :pick @19:35:04.16 SE.seek :beamBreak @19:35:06.31 SE.configure :default @19:35:06.47 Elbow.reconfigure :limp @19:35:06.97 Forearm.reconfigure :loose @19:35:07.02 Forearm.seek 1 @19:35:07.30 Elbow.hold @19:35:07.37 Elbow.reconfigure :tight @19:35:07.47 SE.seek 15.08 Pucks below Top Plate @19:35:07.93 Forearm.force -20 @19:35:07.97 Forearm.seek 1 + 288 counts @19:35:08.27 Forearm.reconfigure :tight @19:35:08.32 Forearm.seek 1 + 450 counts @19:35:08.51 SE.seek 16.08 Pucks below Top Plate @19:35:09.15 Forearm.reconfigure :loose @19:35:09.20 Forearm.seek :retract @19:35:10.18 Elbow.seek CC @19:35:11.90 Elbow.reconfigure :loose @19:35:12.00 Forearm.seek CC @19:35:12.96 Hand.close @19:35:13.64 CC.seek :open @19:35:18.37 Forearm.seek :retract @19:35:19.40 Elbow.reconfigure :tight @19:35:19.45 Elbow.seek 1 @19:35:21.22 Elbow.reconfigure :limp @19:35:21.27 Forearm.seek 1 @19:35:22.26 Elbow.hold @19:35:22.32 Elbow.reconfigure :tight @19:35:22.37 Hand.open @19:35:23.14 Forearm.force -20 @19:35:23.18 Forearm.seek 1 + 318 counts @19:35:23.49 Forearm.reconfigure :tight @19:35:23.54 Forearm.seek 1 + 450 counts @19:35:24.23 SE.seek :down @19:35:25.37
Forearm.reconfigure :loose @19:35:25.42 Forearm.seek :retract @19:35:25.86 SE.stop @19:35:26.34
Tranferring DA @19:35:26.35 Beginning samplePassC @19:35:26.36 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @19:35:26.56 Elbow.seek FlushPuck::Garage @19:35:28.37 Elbow.reconfigure :loose @19:35:28.48 Forearm.seek :garage @19:35:29.43 Hand.close @19:35:30.13 Forearm.seek :retract @19:35:31.12 Elbow.reconfigure :tight @19:35:31.17 Elbow.seek CC @19:35:34.07 Elbow.reconfigure :loose @19:35:34.17 Forearm.reconfigure :tight @19:35:34.22 Forearm.seek CC @19:35:35.45 CC.seek :closed @19:35:39.99 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.38 @19:35:40.05 Hand.open @19:35:40.70 Forearm.reconfigure :loose @19:35:40.74 Forearm.seek :retract @19:35:41.69 PSV.dial PSR @19:35:43.65 Pulling 1.5ml of PSR.flush @19:35:43.66 PSR.select :flush @19:35:43.78 PS.seek 1.5ml @19:35:59.92 delay 5 seconds @19:36:04.94 PSV.dial :waste1 @19:36:05.43 PS.seek 0ml @19:36:21.55 PSV.dial PSR @19:36:22.02 PSR.select :air @19:36:22.14 PS.seek 2.5ml @19:36:48.55 PRV.dial CBV,avoiding: :ab2 @19:36:48.58
CBV.dial PRV @19:36:50.33 PSV.dial :mixloop @19:36:51.36 CTV.dial :air,avoiding: :intake @19:36:51.45 PTV.dial :PRVmixing @19:36:52.76 PS.seek 0ml @19:37:19.17 PSV.dial :air @19:37:19.76 PS.seek 2.5ml @19:37:46.14 PSV.dial :mixloop @19:37:46.60 PS.seek 0ml @19:38:12.98 PSV.dial :air @19:38:13.46 PS.seek 2.5ml @19:38:39.89 PSV.dial :mixloop @19:38:40.36 PS.seek 0ml @19:39:06.67 Garaging FlushPuck in Collection Clamp @19:39:06.75 Elbow.reconfigure :tight @19:39:06.80 Elbow.seek CC @19:39:07.11 Elbow.reconfigure :loose @19:39:07.21 Forearm.seek CC @19:39:08.25 Hand.close @19:39:08.88 CC.seek :open @19:39:13.53 Forearm.seek :retract @19:39:14.52 Elbow.reconfigure :tight @19:39:14.57 Elbow.seek :garage @19:39:17.50 Elbow.reconfigure :loose @19:39:17.60 Forearm.reconfigure :tight @19:39:17.65 Forearm.seek :garage @19:39:18.65 Hand.open @19:39:19.75 Forearm.reconfigure :loose @19:39:19.80 Forearm.seek :retract @19:39:20.80 PSV.dial :mixloop @19:39:20.87 PTV.dial :PRVmixing @19:39:20.95 PRV.dial CBV @19:39:20.98
CBV.dial PRV @19:39:21.18 CTV.dial :air,avoiding: :intake @19:39:21.26 Loading lyfil puck from tube 2 into Collection Clamp @19:39:21.50 SC.reconfigure :tight @19:39:21.55 SC.seek 2 @19:39:25.11 SC.configure :limp @19:39:25.32 Elbow.reconfigure :tight @19:39:25.37 Elbow.seek 2 @19:39:27.28 Forearm.reconfigure :tight @19:39:27.33 Forearm.seek 2 + 450 counts @19:39:28.36 SE.reconfigure :pick @19:39:28.42 SE.seek :beamBreak @19:39:30.47 SE.configure :default @19:39:30.63 Elbow.seek 2 @19:39:30.82 Forearm.seek 2 + 300 counts @19:39:31.00 Forearm.reconfigure :loose @19:39:31.05 Hand.close @19:39:31.64 SE.seek :down @19:39:31.85
Forearm.seek :retract @19:39:32.80 Elbow.seek CC @19:39:33.40 SE.stop @19:39:34.39
Elbow.reconfigure :loose @19:39:34.49 Forearm.reconfigure :tight @19:39:34.95 Forearm.seek CC @19:39:36.18 CC.seek :closed @19:39:40.70 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.16 @19:39:40.72 Hand.open @19:39:41.44 Forearm.reconfigure :loose @19:39:41.49 Forearm.seek :retract @19:39:42.46 CTV.dial CSV,avoiding: :intake @19:39:43.82 CSV.dial CTV @19:39:44.14 PS.seek 2.5ml @19:40:10.51 CS.seek 4.5ml @19:40:25.35 delay 8 seconds @19:40:33.37 PSV.dial :air @19:40:33.84 delay 2 seconds @19:40:35.86 PSV.dial :waste2 @19:40:36.60 PS.seek 0ml @19:41:02.95 CBV.dial between PRV and waste1 @19:41:03.27 CSV.dial :air @19:41:03.73 CS.seek 10ml @19:41:18.57 CSV.dial CTV @19:41:19.03 CS.seek 0ml @19:41:45.66 CBV.dial :waste1 @19:41:45.96 delay 5 seconds @19:41:50.98 CTV.dial :air,avoiding: :intake @19:41:52.39 Storing lyfil puck in Collection Clamp to tube 1 @19:41:52.57 SC.reconfigure :tight @19:41:52.62 SC.seek 1 @19:41:56.20 SC.configure :limp @19:41:56.79 Elbow.reconfigure :tight @19:41:56.96 Elbow.seek 1 @19:41:58.69 Forearm.reconfigure :tight @19:41:58.74 Forearm.seek 1 + 450 counts @19:41:59.77 SE.reconfigure :pick @19:41:59.83 SE.seek :beamBreak @19:42:01.96 SE.configure :default @19:42:02.12 Elbow.reconfigure :limp @19:42:02.17 Forearm.reconfigure :loose @19:42:02.24 Forearm.seek 1 @19:42:02.52 Elbow.hold @19:42:02.58 Elbow.reconfigure :tight @19:42:02.68 SE.seek 16.07 Pucks below Top Plate @19:42:03.24 Forearm.force -20 @19:42:03.29 Forearm.seek 1 + 309 counts @19:42:03.58 Forearm.reconfigure :tight @19:42:03.63 Forearm.seek 1 + 450 counts @19:42:03.82 SE.seek 17.07 Pucks below Top Plate @19:42:04.42 Forearm.reconfigure :loose @19:42:04.47 Forearm.seek :retract @19:42:05.41 Elbow.seek CC @19:42:07.10 Elbow.reconfigure :loose @19:42:07.20 Forearm.seek CC @19:42:08.14 Hand.close @19:42:08.75 CC.seek :open @19:42:13.49 Forearm.seek :retract @19:42:14.44 Elbow.reconfigure :tight @19:42:14.49 Elbow.seek 1 @19:42:16.74 Elbow.reconfigure :limp @19:42:16.80 Forearm.seek 1 @19:42:17.80 Elbow.hold @19:42:17.86 Elbow.reconfigure :tight @19:42:17.92 Hand.open @19:42:18.63 Forearm.force -20 @19:42:18.67 Forearm.seek 1 + 320 counts @19:42:18.98 Forearm.reconfigure :tight @19:42:19.03 Forearm.seek 1 + 450 counts @19:42:19.29 SE.seek :down @19:42:20.43
Forearm.reconfigure :loose @19:42:20.48 Forearm.seek :retract @19:42:20.85 SE.stop @19:42:21.39
PSV.dial :air @19:42:22.14 PS.seek 2.5ml @19:42:48.48 delay 1 second @19:42:49.49 PSV.dial :mixloop @19:42:49.95 delay 1 second @19:42:50.97 PSV.dial :air @19:42:51.43 delay 1 second @19:42:52.45 PSV.dial :mixloop @19:42:52.92 delay 1 second @19:42:53.93 PSV.dial :air @19:42:54.40 delay 1 second @19:42:55.42 PS.seek 0ml @19:43:21.76 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @19:43:21.95 Elbow.seek FlushPuck::Garage @19:43:23.85 Elbow.reconfigure :loose @19:43:23.95 Forearm.seek :garage @19:43:24.92 Hand.close @19:43:25.62 Forearm.seek :retract @19:43:26.60 Elbow.reconfigure :tight @19:43:27.09 Elbow.seek PC @19:43:28.37 Elbow.reconfigure :loose @19:43:28.48 Forearm.reconfigure :tight @19:43:28.53 Forearm.seek PC @19:43:29.75 PC.seek :closed @19:43:34.42 Processing Clamp onSpring/offSpring for 16/26 samples, on/off torque 1.60 @19:43:34.48 Hand.open @19:43:35.19 Forearm.reconfigure :loose @19:43:35.24 Forearm.seek :retract @19:43:36.15 PRV.dial :air @19:43:36.60 PSV.dial :mixloop @19:43:37.14 PS.seek 0.3ml @19:43:41.10 PSV.dial PSR @19:43:42.16 Pulling 0.4ml of PSR.air @19:43:42.17 PSR.select :air @19:43:42.19 PS.seek 0.7ml @19:43:47.13 delay 3 seconds @19:43:50.16 PSR.select :air @19:43:50.18 PS.seek 2.5ml @19:44:09.38 delay 3 seconds @19:44:12.40 PSV.dial :mixloop @19:44:13.46 PTV.dial :mixing @19:44:14.21 PBV.dial :waste2 @19:44:15.15 PS.seek 0ml @19:44:41.70 Garaging FlushPuck in Processing Clamp @19:44:41.77 Elbow.reconfigure :tight @19:44:41.81 Elbow.seek PC @19:44:41.93 Elbow.reconfigure :loose @19:44:42.04 Forearm.seek PC @19:44:43.02 Hand.close @19:44:43.63 PC.seek :open @19:44:48.79 Forearm.seek :retract @19:44:49.74 Elbow.reconfigure :tight @19:44:49.79 Elbow.seek :garage @19:44:51.17 Elbow.reconfigure :loose @19:44:51.27 Forearm.reconfigure :tight @19:44:51.32 Forearm.seek :garage @19:44:52.34 Hand.open @19:44:53.06 Forearm.reconfigure :loose @19:44:53.11 Forearm.seek :retract @19:44:54.13 Loading da puck from tube 2 into Processing Clamp @19:44:54.36 SC.reconfigure :tight @19:44:54.42 SC.seek 2 @19:44:57.98 SC.configure :limp @19:44:58.19 Elbow.reconfigure :tight @19:44:58.24 Elbow.seek 2 @19:45:00.11 Forearm.reconfigure :tight @19:45:00.16 Forearm.seek 2 + 450 counts @19:45:01.22 SE.reconfigure :pick @19:45:01.28 SE.seek :beamBreak @19:45:03.42 SE.configure :default @19:45:03.58 Elbow.seek 2 @19:45:03.76 Forearm.seek 2 + 300 counts @19:45:04.09 Forearm.reconfigure :loose @19:45:04.14 Hand.close @19:45:04.76 SE.seek :down @19:45:04.97
Forearm.seek :retract @19:45:05.99 Elbow.seek PC @19:45:06.60 SE.stop @19:45:08.90
Elbow.reconfigure :loose @19:45:09.07 Forearm.reconfigure :tight @19:45:09.12 Forearm.seek PC @19:45:10.38 PC.seek :closed @19:45:15.11 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.62 @19:45:15.12 Hand.open @19:45:15.81 Forearm.reconfigure :loose @19:45:15.85 Forearm.seek :retract @19:45:16.82 Heating Puck.from 2, :da in Processing Clamp to 28C for 2:00:00 @19:45:16.84 PH.seek 28C,5:00 @19:45:54.29 PH.hold 2:00:00,28C @19:45:54.34 delay 1:30 @19:47:24.38 PSR.select :air @19:47:24.40 PSV.dial PSR @19:47:25.44 Pulling 0.5ml of PSR.wash @19:47:25.45 PSR.select :wash @19:47:25.57 PS.seek 0.5ml @19:47:31.50 delay 5 seconds @19:47:36.52 PSR.select :air @19:47:36.65 PS.seek 2ml @19:47:52.78 PTV.dial :puck @19:47:53.52 PSV.dial PTV @19:47:54.27 PBV.dial :waste1 @19:47:55.66 PS.seek 1.5ml @19:48:01.57 delay 3 seconds @19:48:04.58 PBV.dial between PSV and waste1 @19:48:04.93 PS.seek 0ml @19:48:21.10 delay 5:00 @19:53:21.12 recoverPSRreagent08 @19:53:21.13 PTV.dial :puck @19:53:21.20 PBV.dial :waste1 @19:53:21.56 delay 30 seconds @19:53:51.58 PBV.dial between PSV and waste1 @19:53:51.95 PSR.select :air @19:53:51.97 PSV.dial PSR @19:53:52.72 PS.reconfigure :fast @19:53:52.77 PS.seek 2.5ml @19:54:16.65 PSV.dial PTV @19:54:17.53 delay 5 seconds @19:54:22.55 PSV.dial PSR @19:54:23.28 delay 5 seconds @19:54:28.30 PSV.dial PTV @19:54:29.04 delay 5 seconds @19:54:34.06 PSV.dial :air @19:54:34.81 PS.seek 0ml @19:54:58.18 PTV.dial :PRVmixing,via: PRV @19:54:59.49 PSV.dial :mixloop @19:54:59.96 PRV.dial :ab1 @19:55:00.72 PS.reconfigure :slow @19:55:00.77 PS.seek 0.15ml @19:55:04.40 delay 2 seconds @19:55:06.42 CBV.dial PRV @19:55:06.45
PRV.dial CBV @19:55:07.01 PS.seek 0.35ml @19:55:11.59 delay 2 seconds @19:55:13.60 CBV.dial between PRV and PSV @19:55:13.93 PRV.dial :air @19:55:14.40 PS.seek 0.9ml @19:55:26.52 PRV.dial :waste2 @19:55:27.27 PS.seek 0.5ml @19:55:35.99 addPMLReagent08 @19:55:36.00 PSV.dial :air @19:55:36.48 PS.reconfigure :default @19:55:36.53 PS.seek 2ml @19:55:52.68 PSV.dial :PML @19:55:53.15 PTV.dial :mixing @19:55:53.90 PBV.dial :waste1 @19:55:54.32 PS.seek 1.85ml @19:55:56.77 PBV.dial between PSV and waste1 @19:55:57.14 PS.seek 0ml @19:56:16.87 delay 2 seconds @19:56:18.89 Beginning samplePassC @19:56:18.90 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @19:56:19.09 Elbow.reconfigure :tight @19:56:19.13 Elbow.seek FlushPuck::Garage @19:56:20.50 Elbow.reconfigure :loose @19:56:20.60 Forearm.seek :garage @19:56:21.62 Hand.close @19:56:22.36 Forearm.seek :retract @19:56:23.33 Elbow.reconfigure :tight @19:56:23.39 Elbow.seek CC @19:56:26.28 Elbow.reconfigure :loose @19:56:26.38 Forearm.reconfigure :tight @19:56:26.43 Forearm.seek CC @19:56:27.74 CC.seek :closed @19:56:32.82 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.38 @19:56:32.89 Hand.open @19:56:33.55 Forearm.reconfigure :loose @19:56:33.60 Forearm.seek :retract @19:56:34.60 PSV.dial PSR @19:56:35.65 Pulling 1.5ml of PSR.flush @19:56:35.66 PSR.select :flush @19:56:35.78 PS.seek 1.5ml @19:56:52.02 delay 5 seconds @19:56:57.04 PSV.dial :waste1 @19:56:57.51 PS.seek 0ml @19:57:13.65 PSV.dial PSR @19:57:14.12 PSR.select :air @19:57:14.24 PS.seek 2.5ml @19:57:40.56 PRV.dial CBV,avoiding: :ab2 @19:57:40.59
CBV.dial PRV @19:57:42.19 PSV.dial :mixloop @19:57:43.23 CTV.dial :air,avoiding: :intake @19:57:43.30 PTV.dial :PRVmixing @19:57:44.08 PS.seek 0ml @19:58:10.46 PSV.dial :air @19:58:10.93 PS.seek 2.5ml @19:58:37.33 PSV.dial :mixloop @19:58:37.81 PS.seek 0ml @19:59:04.14 PSV.dial :air @19:59:04.62 PS.seek 2.5ml @19:59:31.02 PSV.dial :mixloop @19:59:31.49 PS.seek 0ml @19:59:57.86 Garaging FlushPuck in Collection Clamp @19:59:57.94 Elbow.reconfigure :tight @19:59:57.99 Elbow.seek CC @19:59:58.31 Elbow.reconfigure :loose @19:59:58.41 Forearm.seek CC @19:59:59.47 Hand.close @20:00:01.11 CC.seek :open @20:00:05.73 Forearm.seek :retract @20:00:06.72 Elbow.reconfigure :tight @20:00:06.77 Elbow.seek :garage @20:00:10.17 Elbow.reconfigure :loose @20:00:10.28 Forearm.reconfigure :tight @20:00:10.33 Forearm.seek :garage @20:00:11.34 Hand.open @20:00:12.05 Forearm.reconfigure :loose @20:00:12.10 Forearm.seek :retract @20:00:13.08 delayUntil "20:04:18.87" @20:00:13.11 Whole Cell Archive sampling 1000ml @20:00:13.13 Loading wcr puck from tube 2 into Collection Clamp @20:00:13.43 SC.reconfigure :tight @20:00:13.49 SC.seek 2 @20:00:13.79 SC.configure :limp @20:00:14.02 Elbow.reconfigure :tight @20:00:14.07 Elbow.seek 2 @20:00:16.02 Forearm.reconfigure :tight @20:00:16.07 Forearm.seek 2 + 450 counts @20:00:17.21 SE.reconfigure :pick @20:00:17.27 SE.seek :beamBreak @20:00:19.50 SE.configure :default @20:00:19.67 Elbow.seek 2 @20:00:19.86 Forearm.seek 2 + 300 counts @20:00:20.06 Forearm.reconfigure :loose @20:00:20.11 Hand.close @20:00:20.75 SE.seek :down @20:00:20.96 Forearm.seek :retract @20:00:21.96 Elbow.seek CC @20:00:22.67 SE.stop @20:00:24.04 Elbow.reconfigure :loose @20:00:24.15 Forearm.reconfigure :tight @20:00:24.21 Forearm.seek CC @20:00:25.44 CC.seek :closed @20:00:29.96 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.28 @20:00:29.98 Hand.open @20:00:30.68 Forearm.reconfigure :loose @20:00:30.73 Forearm.seek :retract @20:00:31.71 collectSample 1000ml w/bubble point of 35psi @20:00:31.72 Priming 7ml @20:00:31.73 Intake.select :close @20:00:31.74 Exhaust.select :close @20:00:31.76 SS.hold @20:00:31.96 Calibrating pressure sensors @20:00:31.97 CTV.dial :air @20:00:32.05 SSV.dial :spit,avoiding: :exhaust @20:00:32.15 SS.reconfigure :fast @20:00:32.20 SS.seek 5ml @20:00:35.03 SSV.dial CBV @20:00:35.06 CBV.dial SSV @20:00:36.42 delay 3.8 seconds @20:00:40.36 Sensor limits: Intake max 116.3psia; Outlet max 108.9psia @20:00:40.37 SSV.dial :spit @20:00:41.69 SS.seek 0ml @20:00:44.36 SSV.dial CBV @20:00:45.68 CTV.dial :intake @20:00:46.44 SSV.dial CBV @20:00:46.47 CBV.dial SSV @20:00:46.61 Allowing 40s for 7.0ml of remaining 7.0ml @20:00:47.08 Intake.select :open @20:00:49.25 Intake @14.6psia, vacuum from 9.1 to 12.1psi @20:00:49.27 SS.reconfigure :inhaling 2.20ml/s @20:00:49.32 SS.seek 7ml @20:00:55.84 Stroke completed -- waiting for pressure to equalize ... @20:00:55.85 SS.reconfigure :exhale @20:00:55.90 SS.hold @20:01:05.68 Intake.select :close @20:01:05.84 SSV.dial :exhaust @20:01:06.62 Exhaust.select :open @20:01:06.73 SS.seek 0ml @20:01:10.76 Exhaust.select :close @20:01:10.87 Sampling 1000.0ml @20:01:10.89 Intake.select :close @20:01:10.90 Exhaust.select :close @20:01:10.91 SS.reconfigure :default @20:01:10.96 SS.hold @20:01:11.16 CTV.dial :intake @20:01:11.25 SSV.dial CBV @20:01:11.28 CBV.dial SSV @20:01:12.51 Allowing 130s for 25.0ml of remaining 1000.0ml @20:01:12.53 Intake.select :open @20:01:14.70 Intake @15.3psia, vacuum from 9.8 to 12.8psi @20:01:14.72 SS.reconfigure :inhaling 2.20ml/s @20:01:14.77 SS.seek 25ml @20:01:23.19 Vacuum too high -- waiting up to 40 seconds @20:01:23.21 SS.reconfigure :exhale @20:01:23.26 SS.hold @20:01:29.30 Intake @16.8psia, vacuum from 10.0 to 13.0psi @20:01:29.32 SS.reconfigure :inhaling 1.76ml/s @20:01:29.37 SS.seek 25ml @20:01:33.64 Vacuum too high -- waiting up to 40 seconds @20:01:33.65 SS.reconfigure :exhale @20:01:33.71 SS.hold @20:01:36.43 Intake @16.0psia, vacuum from 10.0 to 13.0psi @20:01:36.45 SS.reconfigure :inhaling 1.41ml/s @20:01:36.95 SS.seek 25ml @20:01:42.49 Stroke completed -- waiting for pressure to equalize ... @20:01:42.51 SS.reconfigure :exhale @20:01:42.56 SS.hold @20:01:58.79 Intake.select :close @20:01:58.95 SSV.dial :exhaust @20:01:59.71 Exhaust.select :open @20:01:59.84 SS.seek 0ml @20:02:08.81 Exhaust.select :close @20:02:08.92 SSV.dial CBV @20:02:08.95 CBV.dial SSV @20:02:09.70 Allowing 130s for 25.0ml of remaining 975.0ml @20:02:09.71 Intake.select :open @20:02:12.33 Intake @15.6psia, vacuum from 10.0 to 13.0psi @20:02:12.35 SS.reconfigure :inhaling 1.41ml/s @20:02:12.40 SS.seek 25ml @20:02:32.00 Vacuum too high -- waiting up to 40 seconds @20:02:32.02 SS.reconfigure :exhale @20:02:32.08 SS.hold @20:02:39.74 Intake @17.0psia, vacuum from 10.0 to 13.0psi @20:02:39.76 SS.reconfigure :inhaling 1.13ml/s @20:02:39.81 SS.seek 25ml @20:02:40.51 Stroke completed -- waiting for pressure to equalize ... @20:02:40.52 SS.reconfigure :exhale @20:02:40.57 SS.hold @20:02:53.45 Intake.select :close @20:02:53.61 SSV.dial :exhaust @20:02:54.37 Exhaust.select :open @20:02:54.49 SS.seek 0ml @20:03:03.56 Exhaust.select :close @20:03:03.67 SSV.dial CBV @20:03:03.71 CBV.dial SSV @20:03:04.43 Allowing 130s for 25.0ml of remaining 950.0ml @20:03:04.45 Intake.select :open @20:03:06.62 Intake @15.7psia, vacuum from 10.0 to 13.0psi @20:03:06.64 SS.reconfigure :inhaling 1.13ml/s @20:03:06.69 SS.seek 25ml @20:03:31.41 Stroke completed -- waiting for pressure to equalize ... @20:03:31.43 SS.reconfigure :exhale @20:03:31.48 SS.hold @20:03:47.83 Intake.select :close @20:03:47.99 SSV.dial :exhaust @20:03:48.76 Exhaust.select :open @20:03:48.87 SS.seek 0ml @20:03:57.66 Exhaust.select :close @20:03:57.78 SSV.dial CBV @20:03:57.80 CBV.dial SSV @20:03:58.57 Allowing 130s for 25.0ml of remaining 925.0ml @20:03:58.59 Intake.select :open @20:04:00.76 Intake @15.8psia, vacuum from 10.0 to 13.0psi @20:04:00.78 SS.reconfigure :inhaling 1.13ml/s @20:04:00.83 SS.seek 25ml @20:04:18.89 recoverPRVreagent08 @20:04:18.90 PTV.dial :mixing @20:04:19.65 PBV.dial :waste1 @20:04:20.03 delay 30 seconds @20:04:23.25 Vacuum too high -- waiting up to 40 seconds @20:04:23.27 SS.reconfigure :exhale @20:04:23.32 SS.hold @20:04:29.25 Intake @16.1psia, vacuum from 10.0 to 13.0psi @20:04:29.27 SS.reconfigure :inhaling 0.91ml/s @20:04:29.32 SS.seek 25ml @20:04:32.30 Stroke completed -- waiting for pressure to equalize ... @20:04:32.32 SS.reconfigure :exhale @20:04:32.36 SS.hold @20:04:43.57 Intake.select :close @20:04:44.18 SSV.dial :exhaust @20:04:44.95 Exhaust.select :open @20:04:45.07 SS.seek 0ml @20:04:50.05 PBV.dial between PSV and waste1 @20:04:50.42 PSV.dial :air @20:04:51.02 PS.reconfigure :fast @20:04:51.07 PS.seek 2.5ml @20:04:54.18 Exhaust.select :close @20:04:54.29 SSV.dial CBV @20:04:54.33 CBV.dial SSV @20:04:55.08 Allowing 130s for 25.0ml of remaining 900.0ml @20:04:55.09 Intake.select :open @20:04:57.26 Intake @15.8psia, vacuum from 10.0 to 13.0psi @20:04:57.28 SS.reconfigure :inhaling 0.91ml/s @20:04:57.33 SS.seek 25ml @20:05:15.23 PSV.dial :mixloop @20:05:15.69 delay 2 seconds @20:05:17.70 PSV.dial :air @20:05:18.17 delay 2 seconds @20:05:20.19 PSV.dial :mixloop @20:05:20.66 delay 2 seconds @20:05:22.68 PSV.dial :air @20:05:23.14 PS.seek 0ml @20:05:26.46 Stroke completed -- waiting for pressure to equalize ... @20:05:26.47 SS.reconfigure :exhale @20:05:26.52 SS.hold @20:05:39.20 Intake.select :close @20:05:39.36 SSV.dial :exhaust @20:05:40.13 Exhaust.select :open @20:05:40.25 SS.seek 0ml @20:05:46.80 washCycleDA #1 of 2 @20:05:46.81 PSR.select :air @20:05:46.82 PSV.dial PSR @20:05:48.15 Pulling 0.5ml of PSR.wash @20:05:48.16 PSR.select :wash @20:05:48.28 PS.reconfigure :default @20:05:48.33 PS.seek 0.5ml @20:05:49.04 Exhaust.select :close @20:05:49.15 SSV.dial CBV @20:05:49.18 CBV.dial SSV @20:05:49.95 Allowing 130s for 25.0ml of remaining 875.0ml @20:05:49.96 Intake.select :open @20:05:52.12 Intake @15.7psia, vacuum from 10.0 to 13.0psi @20:05:52.14 SS.reconfigure :inhaling 0.91ml/s @20:05:52.19 SS.seek 25ml @20:05:54.20 delay 5 seconds @20:05:59.22 PSR.select :air @20:05:59.34 PS.seek 2ml @20:06:15.51 PTV.dial :puck @20:06:16.26 PSV.dial PTV @20:06:17.01 PBV.dial :waste1 @20:06:17.45 PS.seek 1.5ml @20:06:22.64 Stroke completed -- waiting for pressure to equalize ... @20:06:22.66 SS.reconfigure :exhale @20:06:22.71 SS.hold @20:06:23.42 delay 3 seconds @20:06:26.43 PBV.dial between PSV and waste1 @20:06:26.85 PS.seek 0ml @20:06:37.20 Intake.select :close @20:06:37.36 SSV.dial :exhaust @20:06:38.12 Exhaust.select :open @20:06:38.24 SS.seek 0ml @20:06:43.08 delay 2:00 @20:06:46.96 Exhaust.select :close @20:06:47.07 SSV.dial CBV @20:06:47.09 CBV.dial SSV @20:06:47.85 Allowing 130s for 25.0ml of remaining 850.0ml @20:06:47.86 Intake.select :open @20:06:50.03 Intake @15.6psia, vacuum from 10.0 to 13.0psi @20:06:50.05 SS.reconfigure :inhaling 0.91ml/s @20:06:50.10 SS.seek 25ml @20:07:20.03 Stroke completed -- waiting for pressure to equalize ... @20:07:20.05 SS.reconfigure :exhale @20:07:20.10 SS.hold @20:07:32.90 Intake.select :close @20:07:33.06 SSV.dial :exhaust @20:07:33.83 Exhaust.select :open @20:07:33.95 SS.seek 0ml @20:07:42.77 Exhaust.select :close @20:07:42.88 SSV.dial CBV @20:07:42.92 CBV.dial SSV @20:07:43.68 Allowing 130s for 25.0ml of remaining 825.0ml @20:07:43.69 Intake.select :open @20:07:45.85 Intake @15.6psia, vacuum from 10.0 to 13.0psi @20:07:45.87 SS.reconfigure :inhaling 0.91ml/s @20:07:45.92 SS.seek 25ml @20:08:15.84 Stroke completed -- waiting for pressure to equalize ... @20:08:15.86 SS.reconfigure :exhale @20:08:15.91 SS.hold @20:08:30.40 Intake.select :close @20:08:30.56 SSV.dial :exhaust @20:08:31.33 Exhaust.select :open @20:08:31.45 SS.seek 0ml @20:08:40.22 Exhaust.select :close @20:08:40.33 SSV.dial CBV @20:08:40.36 CBV.dial SSV @20:08:41.13 Allowing 130s for 25.0ml of remaining 800.0ml @20:08:41.14 Intake.select :open @20:08:43.10 recoverPSRreagent08 @20:08:43.11 PTV.dial :puck @20:08:43.32 PBV.dial :waste1 @20:08:43.36 Intake @15.6psia, vacuum from 10.0 to 13.0psi @20:08:43.40 SS.reconfigure :inhaling 0.91ml/s @20:08:43.47 SS.seek 25ml @20:08:43.74 delay 30 seconds @20:09:09.28 Vacuum too high -- waiting up to 40 seconds @20:09:09.29 SS.reconfigure :exhale @20:09:09.35 SS.hold @20:09:13.77 PBV.dial between PSV and waste1 @20:09:14.13 PSR.select :air @20:09:14.15 PSV.dial PSR @20:09:14.92 PS.reconfigure :fast @20:09:14.97 PS.seek 2.5ml @20:09:17.14 Intake @15.9psia, vacuum from 10.0 to 13.0psi @20:09:17.16 SS.reconfigure :inhaling 0.73ml/s @20:09:17.21 SS.seek 25ml @20:09:22.58 Stroke completed -- waiting for pressure to equalize ... @20:09:22.59 SS.reconfigure :exhale @20:09:22.64 SS.hold @20:09:31.60 Intake.select :close @20:09:31.76 SSV.dial :exhaust @20:09:32.53 Exhaust.select :open @20:09:32.65 SS.seek 0ml @20:09:39.07 PSV.dial PTV @20:09:39.84 delay 5 seconds @20:09:41.70 Exhaust.select :close @20:09:41.82 SSV.dial CBV @20:09:41.86 CBV.dial SSV @20:09:42.60 Allowing 130s for 25.0ml of remaining 775.0ml @20:09:42.61 Intake.select :open @20:09:44.78 Intake @15.5psia, vacuum from 10.0 to 13.0psi @20:09:44.80 SS.reconfigure :inhaling 0.73ml/s @20:09:44.85 SS.seek 25ml @20:09:44.90 PSV.dial PSR @20:09:45.65 delay 5 seconds @20:09:50.67 PSV.dial PTV @20:09:51.43 delay 5 seconds @20:09:56.45 PSV.dial :air @20:09:57.20 PS.seek 0ml @20:10:20.70 Stroke completed -- waiting for pressure to equalize ... @20:10:20.72 SS.reconfigure :exhale @20:10:20.76 SS.hold @20:10:20.88 washCycleDA #2 of 2 @20:10:20.89 PSR.select :air @20:10:20.91 PSV.dial PSR @20:10:22.24 Pulling 0.5ml of PSR.wash @20:10:22.25 PSR.select :wash @20:10:22.44 PS.reconfigure :default @20:10:22.49 PS.seek 0.5ml @20:10:28.44 delay 5 seconds @20:10:33.50 PSR.select :air @20:10:33.62 PS.seek 2ml @20:10:37.13 Intake.select :close @20:10:37.29 SSV.dial :exhaust @20:10:38.05 Exhaust.select :open @20:10:38.18 SS.seek 0ml @20:10:47.23 Exhaust.select :close @20:10:47.35 SSV.dial CBV @20:10:47.39 CBV.dial SSV @20:10:48.13 Allowing 130s for 25.0ml of remaining 750.0ml @20:10:48.14 Intake.select :open @20:10:49.79 PTV.dial :puck @20:10:49.86 PSV.dial PTV @20:10:50.31 Intake @15.5psia, vacuum from 10.0 to 13.0psi @20:10:50.33 SS.reconfigure :inhaling 0.73ml/s @20:10:50.37 SS.seek 25ml @20:10:50.62 PBV.dial :waste1 @20:10:51.04 PS.seek 1.5ml @20:10:56.91 delay 3 seconds @20:10:59.93 PBV.dial between PSV and waste1 @20:11:00.29 PS.seek 0ml @20:11:16.60 delay 2:00 @20:11:26.32 Stroke completed -- waiting for pressure to equalize ... @20:11:26.34 SS.reconfigure :exhale @20:11:26.39 SS.hold @20:11:44.97 Intake.select :close @20:11:45.14 SSV.dial :exhaust @20:11:45.90 Exhaust.select :open @20:11:46.02 SS.seek 0ml @20:11:54.81 Exhaust.select :close @20:11:54.92 SSV.dial CBV @20:11:54.95 CBV.dial SSV @20:11:55.72 Allowing 130s for 25.0ml of remaining 725.0ml @20:11:55.73 Intake.select :open @20:11:57.89 Intake @15.4psia, vacuum from 9.9 to 12.9psi @20:11:57.91 SS.reconfigure :inhaling 0.73ml/s @20:11:57.96 SS.seek 25ml @20:12:34.46 Stroke completed -- waiting for pressure to equalize ... @20:12:34.48 SS.reconfigure :exhale @20:12:34.52 SS.hold @20:12:55.42 Intake.select :close @20:12:55.58 SSV.dial :exhaust @20:12:56.35 Exhaust.select :open @20:12:56.47 SS.seek 0ml @20:13:05.48 Exhaust.select :close @20:13:05.59 SSV.dial CBV @20:13:05.62 CBV.dial SSV @20:13:06.39 Allowing 130s for 25.0ml of remaining 700.0ml @20:13:06.40 Intake.select :open @20:13:08.57 Intake @15.5psia, vacuum from 10.0 to 13.0psi @20:13:08.59 SS.reconfigure :inhaling 0.73ml/s @20:13:09.10 SS.seek 25ml @20:13:16.62 recoverPSRreagent08 @20:13:16.63 PTV.dial :puck @20:13:16.71 PBV.dial :waste1 @20:13:17.07 delay 30 seconds @20:13:39.93 Vacuum too high -- waiting up to 40 seconds @20:13:39.95 SS.reconfigure :exhale @20:13:40.00 SS.hold @20:13:47.08 PBV.dial between PSV and waste1 @20:13:47.44 PSR.select :air @20:13:47.46 PSV.dial PSR @20:13:48.23 PS.reconfigure :fast @20:13:48.28 PS.seek 2.5ml @20:13:52.90 Intake @15.2psia, vacuum from 9.7 to 12.7psi @20:13:52.92 SS.reconfigure :inhaling 0.59ml/s @20:13:52.97 SS.seek 25ml @20:13:59.38 Stroke completed -- waiting for pressure to equalize ... @20:13:59.40 SS.reconfigure :exhale @20:13:59.45 SS.hold @20:14:12.50 Intake.select :close @20:14:12.57 PSV.dial PTV @20:14:12.68 SSV.dial :exhaust @20:14:13.45 delay 5 seconds @20:14:13.48 Exhaust.select :open @20:14:13.60 SS.seek 0ml @20:14:18.48 PSV.dial PSR @20:14:19.24 delay 5 seconds @20:14:22.43 Exhaust.select :close @20:14:22.55 SSV.dial CBV @20:14:22.57 CBV.dial SSV @20:14:23.32 Allowing 130s for 25.0ml of remaining 675.0ml @20:14:23.34 Intake.select :open @20:14:24.26 PSV.dial PTV @20:14:25.02 delay 5 seconds @20:14:25.51 Intake @15.3psia, vacuum from 9.8 to 12.8psi @20:14:25.53 SS.reconfigure :inhaling 0.59ml/s @20:14:25.58 SS.seek 25ml @20:14:30.04 PSV.dial :air @20:14:31.26 PS.seek 0ml @20:14:55.06 getPMLReagent08 @20:14:55.07 PRV.dial :air @20:14:55.55 delay 5 seconds @20:15:00.57 PTV.dial :PRVmixing @20:15:01.89 delay 5 seconds @20:15:06.91 PRV.dial :ab2 @20:15:07.36 PTV.dial :PRVmixing @20:15:07.44 PSV.dial :mixloop @20:15:07.91 PS.reconfigure :default @20:15:07.96 PS.seek 0.3ml @20:15:09.67 Vacuum too high -- waiting up to 40 seconds @20:15:09.69 SS.reconfigure :exhale @20:15:09.75 SS.hold @20:15:11.90 delay 2 seconds @20:15:13.97 PRV.dial :air @20:15:14.50 PS.seek 0.55ml @20:15:17.94 addPMLReagent08 @20:15:17.95 PSV.dial :air @20:15:18.52 PS.seek 2ml @20:15:23.09 Intake @15.5psia, vacuum from 10.0 to 13.0psi @20:15:23.11 SS.reconfigure :inhaling 0.47ml/s @20:15:23.16 SS.seek 25ml @20:15:24.85 Stroke completed -- waiting for pressure to equalize ... @20:15:24.87 SS.reconfigure :exhale @20:15:24.91 SS.hold @20:15:34.18 PSV.dial :PML @20:15:34.68 PTV.dial :mixing @20:15:35.47 PBV.dial :waste1 @20:15:35.89 PS.seek 1.85ml @20:15:36.09 Intake.select :close @20:15:36.25 SSV.dial :exhaust @20:15:37.01 Exhaust.select :open @20:15:37.13 SS.seek 0ml @20:15:38.34 PBV.dial between PSV and waste1 @20:15:38.72 PS.seek 0ml @20:15:46.12 Exhaust.select :close @20:15:46.71 SSV.dial CBV @20:15:46.74 CBV.dial SSV @20:15:47.50 Allowing 130s for 25.0ml of remaining 650.0ml @20:15:47.51 Intake.select :open @20:15:49.68 Intake @15.2psia, vacuum from 9.7 to 12.7psi @20:15:49.69 SS.reconfigure :inhaling 0.47ml/s @20:15:49.74 SS.seek 25ml @20:15:58.47 delay 2 seconds @20:16:00.49 delay 8:00 @20:16:45.08 Stroke completed -- waiting for pressure to equalize ... @20:16:45.10 SS.reconfigure :exhale @20:16:45.15 SS.hold @20:17:09.66 Intake.select :close @20:17:09.82 SSV.dial :exhaust @20:17:10.57 Exhaust.select :open @20:17:10.69 SS.seek 0ml @20:17:19.87 Exhaust.select :close @20:17:19.98 SSV.dial CBV @20:17:20.01 CBV.dial SSV @20:17:20.78 Allowing 130s for 25.0ml of remaining 625.0ml @20:17:20.79 Intake.select :open @20:17:22.95 Intake @15.4psia, vacuum from 9.9 to 12.9psi @20:17:22.97 SS.reconfigure :inhaling 0.47ml/s @20:17:23.02 SS.seek 25ml @20:18:17.97 Stroke completed -- waiting for pressure to equalize ... @20:18:17.99 SS.reconfigure :exhale @20:18:18.03 SS.hold @20:18:49.36 Intake.select :close @20:18:49.51 SSV.dial :exhaust @20:18:50.29 Exhaust.select :open @20:18:50.40 SS.seek 0ml @20:18:59.25 Exhaust.select :close @20:18:59.37 SSV.dial CBV @20:18:59.41 CBV.dial SSV @20:19:00.16 Allowing 130s for 25.0ml of remaining 600.0ml @20:19:00.18 Intake.select :open @20:19:02.35 Intake @15.3psia, vacuum from 9.8 to 12.8psi @20:19:02.37 SS.reconfigure :inhaling 0.47ml/s @20:19:02.42 SS.seek 25ml @20:19:39.95 Vacuum too high -- waiting up to 40 seconds @20:19:39.97 SS.reconfigure :exhale @20:19:40.02 SS.hold @20:19:56.82 Intake @15.9psia, vacuum from 10.0 to 13.0psi @20:19:56.84 SS.reconfigure :inhaling 0.38ml/s @20:19:56.89 SS.seek 25ml @20:20:19.63 Stroke completed -- waiting for pressure to equalize ... @20:20:19.64 SS.reconfigure :exhale @20:20:19.69 SS.hold @20:20:42.67 Intake.select :close @20:20:42.83 SSV.dial :exhaust @20:20:43.58 Exhaust.select :open @20:20:43.70 SS.seek 0ml @20:20:52.92 Exhaust.select :close @20:20:53.04 SSV.dial CBV @20:20:53.07 CBV.dial SSV @20:20:53.82 Allowing 130s for 25.0ml of remaining 575.0ml @20:20:53.83 Intake.select :open @20:20:55.99 Intake @15.3psia, vacuum from 9.8 to 12.8psi @20:20:56.01 SS.reconfigure :inhaling 0.38ml/s @20:20:56.06 SS.seek 25ml @20:22:05.44 Stroke completed -- waiting for pressure to equalize ... @20:22:05.45 SS.reconfigure :exhale @20:22:05.50 SS.hold @20:22:44.75 Intake.select :close @20:22:44.91 SSV.dial :exhaust @20:22:45.69 Exhaust.select :open @20:22:45.80 SS.seek 0ml @20:22:54.58 Exhaust.select :close @20:22:54.69 SSV.dial CBV @20:22:54.72 CBV.dial SSV @20:22:55.55 Allowing 130s for 25.0ml of remaining 550.0ml @20:22:55.57 Intake.select :open @20:22:57.74 Intake @15.3psia, vacuum from 9.8 to 12.8psi @20:22:57.76 SS.reconfigure :inhaling 0.38ml/s @20:22:57.81 SS.seek 25ml @20:23:50.85 Vacuum too high -- waiting up to 40 seconds @20:23:50.87 SS.reconfigure :exhale @20:23:50.92 SS.hold @20:24:00.51 recoverPRVreagent08 @20:24:00.52 PTV.dial :mixing @20:24:00.65 PBV.dial :waste1 @20:24:01.01 delay 30 seconds @20:24:11.99 Intake @15.7psia, vacuum from 10.0 to 13.0psi @20:24:12.01 SS.reconfigure :inhaling 0.31ml/s @20:24:12.06 SS.seek 25ml @20:24:31.03 PBV.dial between PSV and waste1 @20:24:31.40 PSV.dial :air @20:24:31.87 PS.reconfigure :fast @20:24:31.92 PS.seek 2.5ml @20:24:35.23 Stroke completed -- waiting for pressure to equalize ... @20:24:35.24 SS.reconfigure :exhale @20:24:35.29 SS.hold @20:24:56.49 PSV.dial :mixloop @20:24:57.02 delay 2 seconds @20:24:59.04 PSV.dial :air @20:24:59.51 delay 2 seconds @20:25:01.52 PSV.dial :mixloop @20:25:01.99 delay 2 seconds @20:25:03.90 Intake.select :close @20:25:04.00 PSV.dial :air @20:25:04.11 SSV.dial :exhaust @20:25:04.48 PS.seek 0ml @20:25:04.89 Exhaust.select :open @20:25:05.01 SS.seek 0ml @20:25:14.14 Exhaust.select :close @20:25:14.25 SSV.dial CBV @20:25:14.27 CBV.dial SSV @20:25:15.05 Allowing 130s for 25.0ml of remaining 525.0ml @20:25:15.06 Intake.select :open @20:25:17.22 Intake @15.2psia, vacuum from 9.7 to 12.7psi @20:25:17.24 SS.reconfigure :inhaling 0.31ml/s @20:25:17.29 SS.seek 25ml @20:25:28.46 washCycleDA #1 of 3 @20:25:28.47 PSR.select :air @20:25:28.49 PSV.dial PSR @20:25:29.82 Pulling 0.5ml of PSR.wash @20:25:29.83 PSR.select :wash @20:25:29.95 PS.reconfigure :default @20:25:30.00 PS.seek 0.5ml @20:25:35.89 delay 5 seconds @20:25:40.92 PSR.select :air @20:25:41.04 PS.seek 2ml @20:25:57.24 PTV.dial :puck @20:25:57.99 PSV.dial PTV @20:25:58.74 PBV.dial :waste1 @20:25:59.16 PS.seek 1.5ml @20:26:05.11 delay 3 seconds @20:26:08.13 PBV.dial between PSV and waste1 @20:26:08.49 PS.seek 0ml @20:26:24.58 delay 2:00 @20:26:50.07 Stroke completed -- waiting for pressure to equalize ... @20:26:50.08 SS.reconfigure :exhale @20:26:50.13 SS.hold @20:27:25.07 SS.reconfigure :default @20:27:25.12 SS.hold @20:27:25.19 Intake.select :close @20:27:25.35 ShallowSampler::Clogged in WCR -- Filtering too Slowly after 500.0ml @20:27:25.37 SSV.dial :exhaust @20:27:26.62 Exhaust.select :open @20:27:26.74 SS.reconfigure :exhale @20:27:26.80 SS.seek 0ml @20:27:35.60 Exhaust.select :close @20:27:35.72 Sampled 500.0ml @20:27:35.74 Drying puck before fixing for 5:00 @20:27:35.75 CBV.dial :waste2b @20:27:36.51 delay 1:00 @20:28:24.61 recoverPSRreagent08 @20:28:24.62 PTV.dial :puck @20:28:24.80 PBV.dial :waste1 @20:28:25.16 delay 30 seconds @20:28:36.53 CSV.dial :air @20:28:36.99 CS.reconfigure :fast @20:28:37.05 CS.seek 10ml @20:28:55.18 PBV.dial between PSV and waste1 @20:28:55.55 PSR.select :air @20:28:55.57 PSV.dial PSR @20:28:56.33 PS.reconfigure :fast @20:28:56.38 PS.seek 2.5ml @20:29:01.50 Pumping wcr puck from tube 2 up to 35psi... @20:29:01.51 CBV.dial SSV @20:29:02.78 CBV.dial between CSV and waste2b @20:29:03.73 CSV.dial between air and CTV @20:29:04.06 CS.seek 7.247ml @20:29:11.15 CTV.dial CSV,avoiding: :air @20:29:11.18 CSV.dial CTV @20:29:11.99 CS.reconfigure :pressurize @20:29:12.05 CS.seek 0ml @20:29:20.42 PSV.dial PTV @20:29:21.19 delay 5 seconds @20:29:26.16 CBV.dial :waste2b @20:29:26.22 PSV.dial PSR @20:29:26.52 CS.reconfigure :default @20:29:26.58 CS.seek 0ml @20:29:26.97 delay 5 seconds @20:29:31.99 PSV.dial PTV @20:29:32.39 delay 1:00 @20:29:32.74 delay 5 seconds @20:29:37.76 PSV.dial :air @20:29:38.63 PS.seek 0ml @20:30:02.11 washCycleDA #2 of 3 @20:30:02.12 PSR.select :air @20:30:02.14 PSV.dial PSR @20:30:03.47 Pulling 0.5ml of PSR.wash @20:30:03.48 PSR.select :wash @20:30:03.60 PS.reconfigure :default @20:30:03.65 PS.seek 0.5ml @20:30:09.64 delay 5 seconds @20:30:14.66 PSR.select :air @20:30:14.78 PS.seek 2ml @20:30:31.48 PTV.dial :puck @20:30:31.56 PSV.dial PTV @20:30:32.41 CTV.dial :air,avoiding: :intake @20:30:32.45 PBV.dial :waste1 @20:30:32.93 PS.seek 1.5ml @20:30:33.80 Fix #1 of 2 (with RNAlater) for 2:30 @20:30:33.82 CSV.dial CSR @20:30:34.27 Pulling 2ml of CSR.RNAlater @20:30:34.28 CSR.select :RNAlater @20:30:34.40 CS.seek 2ml @20:30:38.83 delay 3 seconds @20:30:40.55 delay 5 seconds @20:30:41.84 PBV.dial between PSV and waste1 @20:30:42.21 PS.seek 0ml @20:30:45.56 CSR.select :air @20:30:45.68 CS.reconfigure :fast @20:30:45.73 CS.seek 10ml @20:30:58.30 delay 2:00 @20:31:05.43 Pumping wcr puck from tube 2 up to 35psi... @20:31:05.44 CBV.dial SSV @20:31:06.70 CBV.dial between CSV and waste2b @20:31:07.65 CSV.dial between air and CTV @20:31:08.26 CS.seek 7.213ml @20:31:15.40 CTV.dial CSV,avoiding: :air @20:31:15.42 CSV.dial CTV @20:31:16.82 CS.reconfigure :pressurize @20:31:16.88 CS.seek 0ml @20:31:35.29 delay 2:30 @20:32:58.33 recoverPSRreagent08 @20:32:58.34 PTV.dial :puck @20:32:58.42 PBV.dial :waste1 @20:32:58.78 delay 30 seconds @20:33:28.80 PBV.dial between PSV and waste1 @20:33:29.17 PSR.select :air @20:33:29.19 PSV.dial PSR @20:33:29.92 PS.reconfigure :fast @20:33:29.98 PS.seek 2.5ml @20:33:53.82 PSV.dial PTV @20:33:54.57 delay 5 seconds @20:33:59.59 PSV.dial PSR @20:34:00.34 delay 5 seconds @20:34:05.31 CBV.dial :waste2b @20:34:05.36 PSV.dial PTV @20:34:05.64 CS.reconfigure :default @20:34:05.70 CS.seek 0ml @20:34:06.11 delay 5 seconds @20:34:07.37 delay 1:00 @20:34:11.12 PSV.dial :air @20:34:11.89 PS.seek 0ml @20:34:35.39 washCycleDA #3 of 3 @20:34:35.40 PSR.select :air @20:34:35.41 PSV.dial PSR @20:34:36.73 Pulling 0.5ml of PSR.wash @20:34:36.74 PSR.select :wash @20:34:36.86 PS.reconfigure :default @20:34:36.91 PS.seek 0.5ml @20:34:42.82 delay 5 seconds @20:34:47.84 PSR.select :air @20:34:47.96 PS.seek 2ml @20:35:04.19 PTV.dial :puck @20:35:04.27 PSV.dial PTV @20:35:05.13 PBV.dial :waste1 @20:35:05.56 PS.seek 1.5ml @20:35:07.39 CTV.dial :air,avoiding: :intake @20:35:08.71 Fix #2 of 2 (with RNAlater) for 2:30 @20:35:08.72 CSV.dial CSR @20:35:09.17 Pulling 2ml of CSR.RNAlater @20:35:09.19 CSR.select :RNAlater @20:35:09.31 CS.seek 2ml @20:35:11.54 delay 3 seconds @20:35:14.56 PBV.dial between PSV and waste1 @20:35:14.94 PS.seek 0ml @20:35:15.26 delay 5 seconds @20:35:20.28 CSR.select :air @20:35:20.40 CS.reconfigure :fast @20:35:20.45 CS.seek 10ml @20:35:31.10 delay 2:00 @20:35:40.17 Pumping wcr puck from tube 2 up to 35psi... @20:35:40.19 CBV.dial SSV @20:35:41.44 CBV.dial between CSV and waste2b @20:35:42.40 CSV.dial between air and CTV @20:35:43.00 CS.seek 7.192ml @20:35:50.24 CTV.dial CSV,avoiding: :air @20:35:50.26 CSV.dial CTV @20:35:51.66 CS.reconfigure :pressurize @20:35:51.71 CS.seek 0ml @20:36:07.60 delay 2:30 @20:37:31.12 recoverPSRreagent08 @20:37:31.14 PTV.dial :puck @20:37:31.21 PBV.dial :waste1 @20:37:31.59 delay 30 seconds @20:38:01.61 PBV.dial between PSV and waste1 @20:38:01.99 PSR.select :air @20:38:02.01 PSV.dial PSR @20:38:02.87 PS.reconfigure :fast @20:38:02.92 PS.seek 2.5ml @20:38:27.29 PSV.dial PTV @20:38:28.02 delay 5 seconds @20:38:33.04 PSV.dial PSR @20:38:33.79 delay 5 seconds @20:38:37.62 CBV.dial :waste2b @20:38:37.95 CS.reconfigure :default @20:38:38.01 CS.seek 0ml @20:38:38.81 PSV.dial PTV @20:38:39.57 delay 5 seconds @20:38:41.95 delay 1:00 @20:38:44.59 PSV.dial :air @20:38:45.34 PS.seek 0ml @20:39:08.82 Gate.power :camera,:ON @20:39:08.83 Mixing DA Substrates @20:39:08.84 PSR.select :air @20:39:08.86 PSV.dial PSR @20:39:10.27 Pulling 0.25ml of PSR.sub1 @20:39:10.29 PSR.select :sub1 @20:39:10.50 PS.reconfigure :default @20:39:10.61 PS.seek 0.25ml @20:39:14.07 delay 5 seconds @20:39:19.09 Pulling 0.25ml of PSR.sub2 @20:39:19.10 PSR.select :sub2 @20:39:19.23 PS.seek 0.5ml @20:39:22.60 delay 5 seconds @20:39:27.62 PSR.select :air @20:39:27.73 PS.seek 2ml @20:39:41.97 CTV.dial :air,avoiding: :intake @20:39:43.84 PTV.dial :puck @20:39:43.93 PSV.dial PTV @20:39:44.69 PBV.dial :waste1 @20:39:45.13 PS.seek 1.5ml @20:39:51.04 delay 3 seconds @20:39:54.05 PBV.dial between PSV and waste1 @20:39:54.43 PS.seek 0ml @20:40:10.63 delay 10 seconds @20:40:20.66 recoverPSRreagent08 @20:40:20.67 PTV.dial :puck @20:40:20.74 PBV.dial :waste1 @20:40:21.11 delay 30 seconds @20:40:51.14 PBV.dial between PSV and waste1 @20:40:51.52 PSR.select :air @20:40:51.54 PSV.dial PSR @20:40:52.30 PS.reconfigure :fast @20:40:52.36 PS.seek 2.5ml @20:41:16.69 PSV.dial PTV @20:41:17.46 delay 5 seconds @20:41:22.48 PSV.dial PSR @20:41:23.36 delay 5 seconds @20:41:28.38 PSV.dial PTV @20:41:29.13 delay 5 seconds @20:41:34.15 PSV.dial :air @20:41:34.91 PS.seek 0ml @20:41:58.82 Additional air clear @20:41:58.83 PSR.select :air @20:41:58.84 PSV.dial PSR @20:42:00.19 Pulling 2ml of PSR.air @20:42:00.20 PSR.select :air @20:42:00.22 PS.reconfigure :default @20:42:00.27 PS.seek 2ml @20:42:21.59 delay 5 seconds @20:42:26.62 PTV.dial :puck @20:42:26.70 PSV.dial PTV @20:42:27.47 PBV.dial :waste1 @20:42:28.08 PS.seek 1.5ml @20:42:34.09 delay 3 seconds @20:42:37.11 PBV.dial between PSV and waste1 @20:42:37.49 delay 1 second @20:42:38.51 PSV.dial :waste1 @20:42:39.57 PS.seek 0ml @20:42:55.80 Processing Puck Heater at 28C before imaging @20:42:55.81 PH.coast @20:42:55.93 Imaging da puck in Processing Clamp while storing it to tube 1 @20:42:56.11 SC.reconfigure :tight @20:42:56.16 SC.seek 1 @20:42:59.90 SC.configure :limp @20:43:00.11 Elbow.reconfigure :tight @20:43:00.16 Elbow.seek 1 @20:43:01.93 Forearm.reconfigure :tight @20:43:01.98 Forearm.seek 1 + 450 counts @20:43:03.03 SE.reconfigure :pick @20:43:03.09 SE.seek :beamBreak @20:43:05.12 SE.configure :default @20:43:05.28 Elbow.reconfigure :limp @20:43:05.34 Forearm.reconfigure :loose @20:43:05.39 Forearm.seek 1 @20:43:05.56 Elbow.hold @20:43:05.62 Elbow.reconfigure :tight @20:43:05.73 SE.seek 17.06 Pucks below Top Plate @20:43:06.25 Forearm.force -20 @20:43:06.31 Forearm.seek 1 + 319 counts @20:43:06.62 Forearm.reconfigure :tight @20:43:06.67 Forearm.seek 1 + 450 counts @20:43:06.88 SE.seek 18.06 Pucks below Top Plate @20:43:07.52 Forearm.reconfigure :loose @20:43:07.57 Forearm.seek :retract @20:43:08.56 Elbow.seek PC @20:43:11.25 Elbow.reconfigure :loose @20:43:11.87 Forearm.seek PC @20:43:12.81 Hand.close @20:43:13.45 PC.seek :open @20:43:18.24 Forearm.seek :retract @20:43:19.20 Elbow.reconfigure :tight @20:43:19.25 Elbow.seek 1 @20:43:21.80 Forearm.reconfigure :tight @20:43:21.85 Forearm.seek 1 + 450 counts @20:43:22.86 Hand.open @20:43:23.51 SE.seek 17.71 Pucks below Top Plate @20:43:23.77 SE.reconfigure :seal @20:43:23.83 SE.seek 16.74 Pucks below Top Plate @20:43:24.35 Lights, camera, ACTION! @20:43:24.38 PuckCamera: snap -bin=2,2 -auto /var/log/esp/da23oct0220h625ml.tif @20:43:43.88 Waiting >20 seconds for da to relinquish FlushPuck @20:43:49.54 SX HX9/16 (USB:05): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 21.793 seconds /var/log/esp/da23oct0220h625ml.tif: TIFF Upload Complete @20:43:49.57 PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da23oct0220h625ml15s.tif @20:44:05.20 SX HX9/16 (USB:05): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15 seconds /var/log/esp/midres/da23oct0220h625ml15s.tif: TIFF Upload Complete @20:44:05.22 PuckCamera: snap -bin=2,2 20.0 /var/log/esp/midres/da23oct0220h625ml20s.tif @20:44:25.87 SX HX9/16 (USB:05): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 20 seconds /var/log/esp/midres/da23oct0220h625ml20s.tif: TIFF Upload Complete @20:44:25.89 Gate.power :camera,:OFF @20:44:25.91 DAfans.email "21.793 sec auto-exposure",Subject: "da23oct0220h625ml images!" @20:44:26.35 Elbow.seek 1 @20:44:26.58 Forearm.seek 1 + 450 counts @20:44:26.66 SE.reconfigure :gently @20:44:26.74 SE.seek 17.46 Pucks below Top Plate @20:44:27.47 Elbow.reconfigure :limp @20:44:27.53 Forearm.reconfigure :loose @20:44:27.58 Forearm.seek 1 @20:44:27.86 Elbow.hold @20:44:27.93 Elbow.reconfigure :tight @20:44:28.00 SE.reconfigure :default @20:44:28.05 SE.seek 17.64 Pucks below Top Plate @20:44:28.51 Forearm.force -20 @20:44:28.56 Forearm.seek 1 + 308 counts @20:44:29.42 Forearm.reconfigure :tight @20:44:29.48 Forearm.seek 1 + 450 counts @20:44:30.06 SE.seek :down @20:44:31.32 Forearm.reconfigure :loose @20:44:31.44 Forearm.seek :retract @20:44:31.61 SE.stop @20:44:32.57 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @20:44:33.12 Elbow.seek FlushPuck::Garage @20:44:35.08 Elbow.reconfigure :loose @20:44:35.29 Forearm.seek :garage @20:44:36.36 Hand.close @20:44:37.24 Forearm.seek :retract @20:44:38.29 Elbow.reconfigure :tight @20:44:38.40 Elbow.seek PC @20:44:39.76 Elbow.reconfigure :loose @20:44:39.99 Forearm.reconfigure :tight @20:44:40.09 Forearm.seek PC @20:44:41.73 PC.seek :closed @20:44:46.65 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.62 @20:44:46.79 Hand.open @20:44:47.60 Forearm.reconfigure :loose @20:44:47.72 Forearm.seek :retract @20:44:48.70 PSR.select :air @20:44:48.76 Storing wcr puck in Collection Clamp to tube 1 @20:44:48.80 PSV.dial PSR @20:44:50.50 SC.reconfigure :tight @20:44:50.60 SC.seek 1 @20:44:50.74 Pulling 0.5ml of PSR.flush @20:44:50.78 PSR.select :flush @20:44:50.94 SC.configure :limp @20:44:51.28 PS.seek 0.5ml @20:44:51.57 Elbow.reconfigure :tight @20:44:51.67 Elbow.seek 1 @20:44:54.46 Forearm.reconfigure :tight @20:44:54.56 Forearm.seek 1 + 450 counts @20:44:55.72 SE.reconfigure :pick @20:44:55.86 SE.seek :beamBreak @20:44:57.36 delay 5 seconds @20:44:57.86 SE.configure :default @20:44:58.18 Elbow.reconfigure :limp @20:44:58.30 Forearm.reconfigure :loose @20:44:58.40 Forearm.seek 1 @20:44:58.75 Elbow.hold @20:44:58.89 Elbow.reconfigure :tight @20:44:59.11 SE.seek 18.07 Pucks below Top Plate @20:44:59.83 Forearm.force -20 @20:44:59.91 Forearm.seek 1 + 308 counts @20:45:00.48 Forearm.reconfigure :tight @20:45:00.57 Forearm.seek 1 + 450 counts @20:45:00.93 SE.seek 19.07 Pucks below Top Plate @20:45:01.73 Forearm.reconfigure :loose @20:45:01.83 Forearm.seek :retract @20:45:02.40 PSR.select :air @20:45:03.51 Elbow.seek CC @20:45:03.77 PS.seek 2.5ml @20:45:04.70 upsync @20:45:05.23 Elbow.reconfigure :loose @20:45:05.33 Forearm.seek CC @20:45:06.32 Hand.close @20:45:06.95 CC.seek :open @20:45:11.72 Forearm.seek :retract @20:45:12.70 Elbow.reconfigure :tight @20:45:12.75 Elbow.seek 1 @20:45:14.54 Elbow.reconfigure :limp @20:45:14.59 Forearm.seek 1 @20:45:15.56 Elbow.hold @20:45:15.63 Elbow.reconfigure :tight @20:45:15.68 Hand.open @20:45:16.45 Forearm.force -20 @20:45:16.51 Forearm.seek 1 + 320 counts @20:45:16.85 Forearm.reconfigure :tight @20:45:16.91 Forearm.seek 1 + 450 counts @20:45:17.24 SE.seek :down @20:45:18.39 Forearm.reconfigure :loose @20:45:18.44 Forearm.seek :retract @20:45:18.66 SE.stop @20:45:19.41
Starting finalPurge @20:45:25.12 delay 1 second @20:45:26.14 PRV.dial :waste2,avoiding: :ab2 @20:45:27.98 PTV.dial :PRVmixing @20:45:29.34 PSV.dial :mixloop @20:45:30.38 PS.seek :empty @20:45:38.71
Waiting >20 seconds for psFlush to relinquish FlushPuck @20:45:56.71 PSR.select :air @20:45:56.73 PSV.dial PSR @20:45:57.78 Pulling 0.5ml of PSR.flush @20:45:57.79 PSR.select :flush @20:45:57.91 PS.seek 0.5ml @20:46:03.94 delay 5 seconds @20:46:08.96 PSR.select :air @20:46:09.08 PS.seek 2.5ml @20:46:30.50 delay 1 second @20:46:31.52 PBV.dial :waste1 @20:46:31.89 PTV.dial :mixing @20:46:32.64 PSV.dial :mixloop @20:46:33.69 PS.seek :empty @20:47:00.06 PSV.dial PSR @20:47:01.10 PSR.select :flush @20:47:01.23 PS.seek 1ml @20:47:12.37 PSR.select :air @20:47:12.49 PS.seek 2.5ml @20:47:28.65 PSV.dial PTV @20:47:29.42 PTV.dial :puck @20:47:30.15 PBV.dial :waste1 @20:47:30.33 PS.seek 0ml @20:47:56.71 PSV.dial PSR @20:47:57.46 PSR.select :flush @20:47:57.59 PS.seek 0.5ml @20:48:03.49 PSR.select :air @20:48:03.61 PS.seek 2.5ml @20:48:24.93 PSV.dial PTV @20:48:25.68 PBV.dial PSV @20:48:26.23 PS.seek 0ml @20:48:52.52 PSV.dial PBV @20:48:53.11 PS.seek 2.5ml @20:49:19.65 PSV.dial :waste1 @20:49:20.42 PS.seek 0ml @20:49:46.80 PSV.dial PSR @20:49:47.27 PSR.select :air @20:49:47.30 PS.seek 2.5ml @20:50:13.68 PSV.dial PTV @20:50:14.41 PBV.dial PSV @20:50:14.50 PS.seek 0ml @20:50:40.86 PSV.dial PBV @20:50:41.34 PS.seek 2.5ml @20:51:07.64 PSV.dial :waste1 @20:51:08.39 PS.seek 0ml @20:51:34.83 PSV.dial PSR @20:51:35.30 PSR.select :flush @20:51:35.43 PS.seek 1ml @20:51:46.55 PSR.select :air @20:51:46.67 PS.seek 2.5ml @20:52:02.86 PSV.dial PTV @20:52:03.60 PTV.dial :puck @20:52:03.68 PBV.dial :waste1 @20:52:04.25 PS.seek 0ml @20:52:30.61 PSV.dial PSR @20:52:31.36 PSR.select :flush @20:52:31.49 PS.seek 0.5ml @20:52:37.45 PSR.select :air @20:52:37.57 PS.seek 2.5ml @20:52:58.86 PSV.dial PTV @20:52:59.74 PBV.dial PSV @20:53:00.31 PS.seek 0ml @20:53:26.64 PSV.dial PBV @20:53:27.10 PS.seek 2.5ml @20:53:53.49 PSV.dial :waste1 @20:53:54.23 PS.seek 0ml @20:54:20.55 PSV.dial PSR @20:54:21.02 PSR.select :air @20:54:21.05 PS.seek 2.5ml @20:54:47.48 PSV.dial PTV @20:54:48.22 PBV.dial PSV @20:54:48.41 PS.seek 0ml @20:55:14.98 PSV.dial PBV @20:55:15.46 PS.seek 2.5ml @20:55:41.86 PSV.dial :waste1 @20:55:42.60 PS.seek 0ml @20:56:08.93
Loading FlushPuck into Collection Clamp from Processing Clamp @20:56:09.11 Elbow.seek PC @20:56:11.59 Elbow.reconfigure :loose @20:56:11.69 Forearm.seek PC @20:56:12.64 Hand.close @20:56:13.19 PC.seek :open @20:56:17.91 Forearm.seek :retract @20:56:19.26 Elbow.reconfigure :tight @20:56:19.37 Elbow.seek CC @20:56:23.00 Elbow.reconfigure :loose @20:56:23.11 Forearm.reconfigure :tight @20:56:23.16 Forearm.seek CC @20:56:24.46 CC.seek :closed @20:56:28.99 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.32 @20:56:29.06 Hand.open @20:56:29.75 Forearm.reconfigure :loose @20:56:29.80 Forearm.seek :retract @20:56:30.74 Flushing the Collection Valve::Manifold @20:56:30.87 CSV.dial CSR @20:56:31.32 Pulling 5ml of CSR.flush @20:56:31.33 CSR.select :flush @20:56:31.45 CS.seek 5ml @20:56:45.12 delay 5 seconds @20:56:50.14 CSR.select :air @20:56:50.30 CS.reconfigure :fast @20:56:50.35 CS.seek 7.5ml @20:56:57.05 CSV.dial :waste @20:56:58.34 CS.reconfigure :default @20:56:58.39 CS.seek 0ml @20:57:18.59 Flushing lower manifold's waste1 port @20:57:18.71 CSV.dial :flush @20:57:19.15 CS.reconfigure :fast @20:57:19.20 CS.seek 5ml @20:57:31.90 delay 5 seconds @20:57:36.92 CSV.dial :air @20:57:37.43 CS.seek 7ml @20:57:43.18 CSV.dial CTV @20:57:43.22
CTV.dial CSV @20:57:44.63 CBV.dial :waste1 @20:57:45.39 CS.seek 0ml @20:58:01.99 Flushing lower manifold's waste2 port @20:58:02.57 CSV.dial :flush @20:58:03.40 CS.seek 5ml @20:58:16.18 delay 5 seconds @20:58:21.20 CSV.dial :air @20:58:21.71 CS.seek 7ml @20:58:27.27 CSV.dial CTV @20:58:27.30
CTV.dial CSV @20:58:27.79 CBV.dial :waste2 @20:58:28.84 CS.seek 0ml @20:58:45.46 Flushing lower manifold's waste2b port @20:58:45.58 CSV.dial :flush @20:58:46.32 CS.seek 5ml @20:58:59.11 delay 5 seconds @20:59:04.13 CSV.dial :air @20:59:04.66 CS.seek 7ml @20:59:10.24 CSV.dial CTV @20:59:10.27
CTV.dial CSV @20:59:10.71 CBV.dial :waste2b @20:59:11.75 CS.seek 0ml @20:59:28.24 Disinfecting external Intake with kill @20:59:28.36 CSV.dial CSR @20:59:28.80 Pulling 8ml of CSR.kill @20:59:28.81 CSR.select :kill @20:59:28.93 CS.seek 8ml @20:59:48.74 delay 5 seconds @20:59:53.76 CSR.select :air @20:59:53.88 CS.seek 10ml @20:59:59.36 CSV.dial CBV @20:59:59.39
CBV.dial CSV @21:00:00.44 CTV.dial :intake @21:00:01.19 Intake.select :open @21:00:01.30 CS.seek 2ml @21:00:20.17 Intake.select :close @21:00:20.28 CTV.dial :air @21:00:21.04 CS.reconfigure :default @21:00:21.09 CS.seek 0ml @21:00:26.95 CSV.dial CSR @21:00:27.97 Pulling 5ml of CSR.flush @21:00:27.98 CSR.select :flush @21:00:28.10 CS.seek 5ml @21:00:41.92 delay 5 seconds @21:00:46.94 CSR.select :air @21:00:47.07 CS.seek 10ml @21:01:00.74 CSV.dial CBV @21:01:01.74 CS.seek 0ml @21:01:28.14 Garaging FlushPuck in Collection Clamp @21:01:28.21 Elbow.reconfigure :tight @21:01:28.26 Elbow.seek CC @21:01:28.95 Elbow.reconfigure :loose @21:01:29.06 Forearm.seek CC @21:01:30.09 Hand.close @21:01:30.69 CC.seek :open @21:01:35.39 Forearm.seek :retract @21:01:36.45 Elbow.reconfigure :tight @21:01:36.50 Elbow.seek :garage @21:01:39.44 Elbow.reconfigure :loose @21:01:39.55 Forearm.reconfigure :tight @21:01:39.60 Forearm.seek :garage @21:01:40.64 Hand.open @21:01:41.37 Forearm.reconfigure :loose @21:01:41.41 Forearm.seek :retract @21:01:42.45 Completed [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] begun 18:42:53 @21:01:42.51 Asleep after completing DA_WCR_100223 assays @21:01:42.53 GoodNews.email "{1 => 20,\n 2 => 13,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@20:52:02, 25% flow, 20.8C, 13% humidity, 14.7psia, 13.759V, 0.321A, 85.705Ah, 4.41W\nSampled 500.0ml",Subject: "Asleep after completing DA_WCR_100223 assays" @21:01:46.78 upsync + ESPwhich DA_WCR_100323.rb + fn=DA_WCR_100323.rb + IFS=: + set /home/esp/esp2/mission . + fullPath=/home/esp/esp2/mission/DA_WCR_100323.rb + [ -f /home/esp/esp2/mission/DA_WCR_100323.rb ] + realpath /home/esp/esp2/mission/DA_WCR_100323.rb + return 0 + scriptPath=/home/esp/esp2local/mission/DA_WCR_100323.rb + exec ruby /home/esp/esp2/lib/esp.rb /home/esp/esp2local/mission/DA_WCR_100323.rb @16:50:44.15UTC03-Oct-23
ESP2 v3.42 I2C Gateway v3.20 for core bus @16:50:46.94 Configuration for short puck jake 1/11/21 by brent@mbari.org /home/esp/esp2/type/shallow/jake/configure.rb @16:50:50.70 Polling Can every 10:00 @16:50:50.79 Cleanly exited at 16:48:05UTC03-Oct-23 @16:50:50.85 Loading /home/esp/esp2local/mission/DA_WCR_100323.rb mission @16:50:50.90 GoodNews.email "Starting DA_WCR_100323 w/pucks in tube 2\n{1 => 20,\n 2 => 13,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@16:50:50, 26% flow, 19.1C, 14% humidity, 14.6psia, 13.769V, 0.248A, 90.102Ah, 3.42W",Subject: "DA_WCR_100323 begun" @16:50:50.93
Preparing to sleep until + 00:00:05 @16:50:50.98 Asleep til + 00:00:05 @16:50:51.00 Continuing at 16:50 @16:50:51.29 Gate.power :core,:OFF @16:50:51.30 Gate.power :raw,:OFF @16:50:51.31 Shutdown @16:50:53.62 GoodNews.email "Then, in tube 2: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_100323.rb:14\n{1 => 20,\n 2 => 13,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@16:50:51, 26% flow, 19.2C, 14% humidity, 14.6psia, 13.773V, 0.248A, 90.102Ah, 3.42W",Subject: "Asleep until 16:50" @16:50:55.00 upsync @16:50:55.87
Powering core bus @16:50:57.72 upsync @16:51:01.39
Gate.power :raw,:ON @16:51:02.11 CS.configure :default @16:51:02.16 PS.configure :default @16:51:02.21 SS.configure :default @16:51:02.25 CC.configure :default @16:51:02.30 PC.configure :default @16:51:02.37 Forearm.configure :loose @16:51:02.42 Elbow.configure :loose @16:51:02.49 SC.configure :loose @16:51:02.54 SE.configure :default @16:51:02.59 CH.configure :default @16:51:02.62 PH.configure :default @16:51:02.66 All dwarf controllers running firmware version 3.85 @16:51:02.77 Valve.reset @16:51:03.02 Intake.select :close @16:51:03.08 Exhaust.select :close @16:51:03.09 CTV.dial :air @16:51:03.16 PSV.dial :air @16:51:03.30
Forearm.home.seek :retract @16:51:03.36 CSV.dial :waste1 @16:51:03.79 SSV.dial :spit,avoiding: :exhaust @16:51:04.00 Hand.close @16:51:04.04
Elbow.reconfigure :loose @16:51:04.12 Elbow.jog 1500 @16:51:04.27 PS.home.seek 0ml @16:51:04.66 CS.reconfigure :fast @16:51:04.70 SS.home.seek 0ml @16:51:04.80 CS.home.seek 0ml @16:51:04.88
Elbow.reconfigure :loose @16:51:04.93 Elbow.home.seek :clear @16:51:08.11 SE.home.seek :down @16:51:08.75 SC.reconfigure :tight @16:51:08.80 SC.home.seek :initial @16:51:12.52 PS.stop @16:51:37.18
SC.configure :limp @16:51:37.23 Forearm.seek :retract @16:51:37.30 Elbow.seek :hammer @16:51:38.31 Forearm.reconfigure :tight @16:51:38.36 Forearm.seek retracted @16:51:39.06 PC.home.seek :open @16:51:39.16
CC.home.seek :open @16:51:42.59 Phase [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] @16:51:42.61 Notice.email "At 16:51:42 began in tube 2: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_100323.rb:14\n{1 => 20,\n 2 => 13,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@16:51:42, 26% flow, 19.1C, 14% humidity, 14.6psia, 13.759V, 0.266A, 90.107Ah, 3.66W",Subject: "Started DA_WCR_100323 phase initialPurge; da [1000, 1000] #da and wsr are run simultanelously" @16:51:42.63
Starting initialPurge @16:51:42.65 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @16:51:42.72 Hand.open @16:51:43.45 Elbow.reconfigure :tight @16:51:43.51 Elbow.seek FlushPuck::Garage @16:51:45.43 Elbow.reconfigure :loose @16:51:45.55 Forearm.reconfigure :loose @16:51:45.60 Forearm.seek :garage @16:51:46.48 upsync @16:51:46.58
Hand.close @16:51:47.32 Forearm.seek :retract @16:51:48.34 Elbow.reconfigure :tight @16:51:48.39 Elbow.seek CC @16:51:51.30 Elbow.reconfigure :loose @16:51:51.41 Forearm.reconfigure :tight @16:51:51.46 Forearm.seek CC @16:51:52.75 CC.seek :closed @16:51:57.33 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.40 @16:51:57.39 Hand.open @16:51:58.12 Forearm.reconfigure :loose @16:51:58.17 Forearm.seek :retract @16:51:59.20 Flushing the Collection Valve::Manifold @16:51:59.33 CSV.dial CSR @16:52:00.61 Pulling 5ml of CSR.flush CSR.select :flush @16:52:00.73 CS.reconfigure :default @16:52:01.19 CS.seek 5ml @16:52:15.01 delay 5 seconds @16:52:20.03 CSR.select :air @16:52:20.19 CS.reconfigure :fast @16:52:20.24 CS.seek 7.5ml @16:52:27.16 CSV.dial :waste @16:52:28.45 CS.reconfigure :default @16:52:28.50 CS.seek 0ml @16:52:48.51 Purging external Intake @16:52:48.63 CSV.dial :flush @16:52:49.08 CS.reconfigure :fast @16:52:49.12 CS.seek 10ml @16:53:13.85 delay 5 seconds @16:53:18.87 CSV.dial CBV @16:53:18.91
CBV.dial CSV @16:53:19.65 CTV.dial :intake @16:53:20.41 Intake.select :open @16:53:20.53 CS.seek 0ml @16:53:43.97 Intake.select :close @16:53:44.08 CTV.dial :air @16:53:44.85 Flushing upper manifold @16:53:44.98 CSV.dial :flush @16:53:45.72 CS.seek 5ml @16:53:58.35 delay 5 seconds @16:54:03.37 CSV.dial :air @16:54:03.87 CS.seek 7.001ml @16:54:09.45 CSV.dial CBV @16:54:09.47
CBV.dial CSV @16:54:10.49 CTV.dial :air @16:54:10.51 CS.seek 0ml @16:54:27.07 delay 7 seconds @16:54:34.10 CS.seek 3ml @16:54:42.10 CSV.dial :waste @16:54:42.56 CS.seek 0ml @16:54:50.40 CSV.dial :flush @16:54:50.84 CS.seek 3ml @16:54:58.85 delay 5 seconds @16:55:03.87 CSV.dial :air @16:55:04.37 CS.seek 5ml @16:55:09.88 CSV.dial :air @16:55:09.96 CS.seek 0ml @16:55:22.07 delay 7 seconds @16:55:29.10 CS.seek 3ml @16:55:37.04 CSV.dial :waste @16:55:37.78 CS.seek 0ml @16:55:45.48 Garaging FlushPuck in Collection Clamp @16:55:45.55 Elbow.reconfigure :tight @16:55:45.59 Elbow.seek CC @16:55:45.85 Elbow.reconfigure :loose @16:55:45.95 Forearm.seek CC @16:55:46.99 Hand.close @16:55:47.56 CC.seek :open @16:55:52.62 Forearm.seek :retract @16:55:53.57 Elbow.reconfigure :tight @16:55:53.62 Elbow.seek :garage @16:55:56.57 Elbow.reconfigure :loose @16:55:56.67 Forearm.reconfigure :tight @16:55:56.72 Forearm.seek :garage @16:55:57.70 Hand.open @16:55:58.37 Forearm.reconfigure :loose @16:55:58.41 Forearm.seek :retract @16:55:59.37 da sampling at most 1000ml, wcr at most 1000ml @16:55:59.41 Loading sh1 puck from tube 2 into Collection Clamp @16:55:59.43 DAfans.email "Started da in tube 2 at 16:55:59UTC03-Oct-23\n{1 => 20,\n 2 => 13,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@16:51:42, 26% flow, 19.1C, 14% humidity, 14.6psia, 13.759V, 0.266A, 90.107Ah, 3.66W",Subject: "da sampling at most 1000ml, wcr at most 1000ml" @16:55:59.71
SC.reconfigure :tight @16:55:59.77 SC.seek 2 @16:56:03.32 upsync @16:56:06.54
SC.configure :limp @16:56:06.77 Elbow.reconfigure :tight @16:56:06.82 Elbow.seek 2 @16:56:08.85 Forearm.reconfigure :tight @16:56:08.90 Forearm.seek 2 + 450 counts @16:56:10.04 SE.reconfigure :pick @16:56:10.10 SE.seek :beamBreak @16:56:12.40 SE.configure :default @16:56:12.57 Elbow.seek 2 @16:56:13.10 Forearm.seek 2 + 300 counts @16:56:13.34 Forearm.reconfigure :loose @16:56:13.38 Hand.close @16:56:14.07 SE.seek :down @16:56:14.28
Forearm.seek :retract @16:56:15.69 Elbow.seek CC @16:56:16.05 SE.stop @16:56:17.38
Elbow.reconfigure :loose @16:56:17.48 Forearm.reconfigure :tight @16:56:17.53 Forearm.seek CC @16:56:18.77 CC.seek :closed @16:56:23.29 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.24 @16:56:23.31 Hand.open @16:56:23.99 Forearm.reconfigure :loose @16:56:24.04 Forearm.seek :retract @16:56:25.00 collectSample 1000ml w/bubble point of 24psi @16:56:25.01 Priming 7ml @16:56:25.02 Intake.select :close @16:56:25.03 Exhaust.select :close @16:56:25.04 SS.hold @16:56:25.25 Calibrating pressure and temperature sensors @16:56:25.28 CTV.dial :air @16:56:25.29 SSV.dial :spit,avoiding: :exhaust @16:56:25.48 SS.reconfigure :fast @16:56:25.53 SS.seek 5ml @16:56:28.32 SSV.dial CBV @16:56:28.34
CBV.dial SSV @16:56:29.69 delay 3.8 seconds @16:56:33.62 Sensor limits: Intake max 115.5psia; Outlet max 108.4psia @16:56:33.63 SSV.dial :spit @16:56:34.95 SS.seek 0ml @16:56:37.47 SSV.dial CBV @16:56:38.79 CTV.dial :intake @16:56:39.57 SSV.dial CBV @16:56:39.59
CBV.dial SSV @16:56:39.68 Allowing 40s for 7.0ml of remaining 7.0ml @16:56:39.69 Intake.select :open @16:56:41.85 Intake @14.6psia, vacuum from 9.1 to 12.1psi @16:56:41.87 SS.reconfigure :inhaling 2.20ml/s @16:56:41.92 SS.seek 7ml @16:56:48.48 Stroke completed -- waiting for pressure to equalize ... @16:56:48.49 SS.reconfigure :exhale @16:56:48.54 SS.hold @16:56:53.58 Intake.select :close @16:56:53.73 SSV.dial :exhaust @16:56:54.49 Exhaust.select :open @16:56:54.60 SS.seek 0ml @16:56:58.54 Exhaust.select :close @16:56:58.65 Sampling 1000.0ml @16:56:58.66 Intake.select :close @16:56:58.68 Exhaust.select :close @16:56:58.69 SS.reconfigure :default @16:56:58.73 SS.hold @16:56:58.94 CTV.dial :intake @16:56:59.18 SSV.dial CBV @16:56:59.22
CBV.dial SSV @16:56:59.96 Allowing 130s for 25.0ml of remaining 1000.0ml @16:56:59.97 Intake.select :open @16:57:02.13 Intake @14.9psia, vacuum from 9.4 to 12.4psi @16:57:02.15 SS.reconfigure :inhaling 2.20ml/s @16:57:02.20 SS.seek 25ml @16:57:11.48 Vacuum too high -- waiting up to 40 seconds @16:57:11.49 SS.reconfigure :exhale @16:57:11.55 SS.hold @16:57:16.05 Intake @14.4psia, vacuum from 8.9 to 11.9psi @16:57:16.06 SS.reconfigure :inhaling 1.76ml/s @16:57:16.11 SS.seek 25ml @16:57:23.33 Stroke completed -- waiting for pressure to equalize ... @16:57:23.34 SS.reconfigure :exhale @16:57:23.39 SS.hold @16:57:31.04 Intake.select :close @16:57:31.20 SSV.dial :exhaust @16:57:31.97 Exhaust.select :open @16:57:32.08 SS.seek 0ml @16:57:40.83 Exhaust.select :close @16:57:40.94 SSV.dial CBV @16:57:40.98
CBV.dial SSV @16:57:41.72 Allowing 130s for 25.0ml of remaining 975.0ml @16:57:41.73 Intake.select :open @16:57:43.90 Intake @15.5psia, vacuum from 10.0 to 13.0psi @16:57:43.92 SS.reconfigure :inhaling 1.76ml/s @16:57:43.97 SS.seek 25ml @16:57:58.20 Vacuum too high -- waiting up to 40 seconds @16:57:58.21 SS.reconfigure :exhale @16:57:58.26 SS.hold @16:58:03.37 Intake @14.7psia, vacuum from 9.2 to 12.2psi @16:58:03.39 SS.reconfigure :inhaling 1.41ml/s @16:58:03.43 SS.seek 25ml @16:58:07.92 Stroke completed -- waiting for pressure to equalize ... @16:58:07.93 SS.reconfigure :exhale @16:58:07.98 SS.hold @16:58:14.04 Intake.select :close @16:58:14.19 SSV.dial :exhaust @16:58:14.95 Exhaust.select :open @16:58:15.07 SS.seek 0ml @16:58:23.98 Exhaust.select :close @16:58:24.09 SSV.dial CBV @16:58:24.12
CBV.dial SSV @16:58:24.88 Allowing 130s for 25.0ml of remaining 950.0ml @16:58:24.89 Intake.select :open @16:58:27.05 Intake @15.5psia, vacuum from 10.0 to 13.0psi @16:58:27.07 SS.reconfigure :inhaling 1.41ml/s @16:58:27.12 SS.seek 25ml @16:58:47.50 Stroke completed -- waiting for pressure to equalize ... @16:58:47.51 SS.reconfigure :exhale @16:58:47.55 SS.hold @16:58:59.12 Intake.select :close @16:58:59.27 SSV.dial :exhaust @16:59:00.01 Exhaust.select :open @16:59:00.13 SS.seek 0ml @16:59:09.08 Exhaust.select :close @16:59:09.19 SSV.dial CBV @16:59:09.21
CBV.dial SSV @16:59:09.97 Allowing 130s for 25.0ml of remaining 925.0ml @16:59:09.98 Intake.select :open @16:59:12.14 Intake @15.4psia, vacuum from 9.9 to 12.9psi @16:59:12.16 SS.reconfigure :inhaling 1.41ml/s @16:59:12.21 SS.seek 25ml @16:59:32.81 Stroke completed -- waiting for pressure to equalize ... @16:59:32.82 SS.reconfigure :exhale @16:59:32.87 SS.hold @16:59:44.00 Intake.select :close @16:59:44.15 SSV.dial :exhaust @16:59:44.92 Exhaust.select :open @16:59:45.03 SS.seek 0ml @16:59:53.81 Exhaust.select :close @16:59:53.92 SSV.dial CBV @16:59:53.95
CBV.dial SSV @16:59:54.70 Allowing 130s for 25.0ml of remaining 900.0ml @16:59:54.71 Intake.select :open @16:59:56.87 Intake @15.5psia, vacuum from 10.0 to 13.0psi @16:59:56.89 SS.reconfigure :inhaling 1.41ml/s @16:59:56.94 SS.seek 25ml @17:00:17.78 Stroke completed -- waiting for pressure to equalize ... @17:00:17.79 SS.reconfigure :exhale @17:00:17.83 SS.hold @17:00:30.76 Intake.select :close @17:00:30.92 SSV.dial :exhaust @17:00:31.68 Exhaust.select :open @17:00:31.80 SS.seek 0ml @17:00:40.60 Exhaust.select :close @17:00:40.72 SSV.dial CBV @17:00:40.74
CBV.dial SSV @17:00:41.50 Allowing 130s for 25.0ml of remaining 875.0ml @17:00:41.51 Intake.select :open @17:00:43.67 Intake @15.5psia, vacuum from 10.0 to 13.0psi @17:00:43.69 SS.reconfigure :inhaling 1.41ml/s @17:00:43.74 SS.seek 25ml @17:01:03.53 Vacuum too high -- waiting up to 40 seconds @17:01:03.55 SS.reconfigure :exhale @17:01:03.60 SS.hold @17:01:10.35 Intake @14.6psia, vacuum from 9.1 to 12.1psi @17:01:10.37 SS.reconfigure :inhaling 1.13ml/s @17:01:10.41 SS.seek 25ml @17:01:11.77 Stroke completed -- waiting for pressure to equalize ... @17:01:11.78 SS.reconfigure :exhale @17:01:11.83 SS.hold @17:01:17.48 Intake.select :close @17:01:17.63 SSV.dial :exhaust @17:01:18.39 Exhaust.select :open @17:01:18.50 SS.seek 0ml @17:01:27.18 Exhaust.select :close @17:01:27.29 SSV.dial CBV @17:01:27.32
CBV.dial SSV @17:01:28.07 Allowing 130s for 25.0ml of remaining 850.0ml @17:01:28.09 Intake.select :open @17:01:30.25 Intake @15.5psia, vacuum from 10.0 to 13.0psi @17:01:30.27 SS.reconfigure :inhaling 1.13ml/s @17:01:30.32 SS.seek 25ml @17:01:55.32 Stroke completed -- waiting for pressure to equalize ... @17:01:55.33 SS.reconfigure :exhale @17:01:55.38 SS.hold @17:02:06.94 Intake.select :close @17:02:07.09 SSV.dial :exhaust @17:02:07.85 Exhaust.select :open @17:02:07.97 SS.seek 0ml @17:02:16.98 Exhaust.select :close @17:02:17.09 SSV.dial CBV @17:02:17.12
CBV.dial SSV @17:02:17.87 Allowing 130s for 25.0ml of remaining 825.0ml @17:02:17.89 Intake.select :open @17:02:20.05 Intake @15.5psia, vacuum from 10.0 to 13.0psi @17:02:20.07 SS.reconfigure :inhaling 1.13ml/s @17:02:20.12 SS.seek 25ml @17:02:44.51 Stroke completed -- waiting for pressure to equalize ... @17:02:44.52 SS.reconfigure :exhale @17:02:44.56 SS.hold @17:02:57.67 Intake.select :close @17:02:57.82 SSV.dial :exhaust @17:02:58.57 Exhaust.select :open @17:02:58.69 SS.seek 0ml @17:03:07.65 Exhaust.select :close @17:03:07.76 SSV.dial CBV @17:03:07.80
CBV.dial SSV @17:03:08.53 Allowing 130s for 25.0ml of remaining 800.0ml @17:03:08.54 Intake.select :open @17:03:10.70 Intake @15.3psia, vacuum from 9.8 to 12.8psi @17:03:10.72 SS.reconfigure :inhaling 1.13ml/s @17:03:10.77 SS.seek 25ml @17:03:35.27 Stroke completed -- waiting for pressure to equalize ... @17:03:35.28 SS.reconfigure :exhale @17:03:35.33 SS.hold @17:03:51.55 Intake.select :close @17:03:51.70 SSV.dial :exhaust @17:03:52.45 Exhaust.select :open @17:03:52.56 SS.seek 0ml @17:04:01.23 Exhaust.select :close @17:04:01.34 SSV.dial CBV @17:04:01.36
CBV.dial SSV @17:04:02.12 Allowing 130s for 25.0ml of remaining 775.0ml @17:04:02.13 Intake.select :open @17:04:04.72 Intake @15.3psia, vacuum from 9.8 to 12.8psi @17:04:04.74 SS.reconfigure :inhaling 1.13ml/s @17:04:04.79 SS.seek 25ml @17:04:27.22 Vacuum too high -- waiting up to 40 seconds @17:04:27.23 SS.reconfigure :exhale @17:04:27.29 SS.hold @17:04:35.97 Intake @14.6psia, vacuum from 9.1 to 12.1psi @17:04:35.99 SS.reconfigure :inhaling 0.91ml/s @17:04:36.03 SS.seek 25ml @17:04:39.19 Stroke completed -- waiting for pressure to equalize ... @17:04:39.20 SS.reconfigure :exhale @17:04:39.25 SS.hold @17:04:47.77 Intake.select :close @17:04:47.92 SSV.dial :exhaust @17:04:48.70 Exhaust.select :open @17:04:48.81 SS.seek 0ml @17:04:57.52 Exhaust.select :close @17:04:57.63 SSV.dial CBV @17:04:57.66
CBV.dial SSV @17:04:58.42 Allowing 130s for 25.0ml of remaining 750.0ml @17:04:58.43 Intake.select :open @17:05:00.59 Intake @15.3psia, vacuum from 9.8 to 12.8psi @17:05:00.61 SS.reconfigure :inhaling 0.91ml/s @17:05:00.66 SS.seek 25ml @17:05:30.50 Stroke completed -- waiting for pressure to equalize ... @17:05:30.51 SS.reconfigure :exhale @17:05:30.56 SS.hold @17:05:46.87 Intake.select :close @17:05:47.03 SSV.dial :exhaust @17:05:47.79 Exhaust.select :open @17:05:47.90 SS.seek 0ml @17:05:56.61 Exhaust.select :close @17:05:56.72 SSV.dial CBV @17:05:56.75
CBV.dial SSV @17:05:57.93 Allowing 130s for 25.0ml of remaining 725.0ml @17:05:57.94 Intake.select :open @17:06:00.10 Intake @15.3psia, vacuum from 9.8 to 12.8psi @17:06:00.12 SS.reconfigure :inhaling 0.91ml/s @17:06:00.17 SS.seek 25ml @17:06:28.65 Vacuum too high -- waiting up to 40 seconds @17:06:28.67 SS.reconfigure :exhale @17:06:28.72 SS.hold @17:06:38.65 Intake @14.7psia, vacuum from 9.2 to 12.2psi @17:06:38.67 SS.reconfigure :inhaling 0.73ml/s @17:06:38.71 SS.seek 25ml @17:06:41.13 Stroke completed -- waiting for pressure to equalize ... @17:06:41.14 SS.reconfigure :exhale @17:06:41.18 SS.hold @17:06:49.81 Intake.select :close @17:06:49.96 SSV.dial :exhaust @17:06:50.71 Exhaust.select :open @17:06:50.82 SS.seek 0ml @17:06:59.57 Exhaust.select :close @17:06:59.68 SSV.dial CBV @17:06:59.70
CBV.dial SSV @17:07:00.46 Allowing 130s for 25.0ml of remaining 700.0ml @17:07:00.47 Intake.select :open @17:07:02.63 Intake @15.2psia, vacuum from 9.7 to 12.7psi @17:07:02.65 SS.reconfigure :inhaling 0.73ml/s @17:07:02.70 SS.seek 25ml @17:07:39.13 Stroke completed -- waiting for pressure to equalize ... @17:07:39.14 SS.reconfigure :exhale @17:07:39.19 SS.hold @17:07:57.52 Intake.select :close @17:07:58.08 SSV.dial :exhaust @17:07:58.86 Exhaust.select :open @17:07:58.98 SS.seek 0ml @17:08:07.96 Exhaust.select :close @17:08:08.07 SSV.dial CBV @17:08:08.09
CBV.dial SSV @17:08:08.86 Allowing 130s for 25.0ml of remaining 675.0ml @17:08:08.88 Intake.select :open @17:08:11.04 Intake @15.2psia, vacuum from 9.7 to 12.7psi @17:08:11.06 SS.reconfigure :inhaling 0.73ml/s @17:08:11.11 SS.seek 25ml @17:08:47.14 Stroke completed -- waiting for pressure to equalize ... @17:08:47.15 SS.reconfigure :exhale @17:08:47.20 SS.hold @17:09:09.75 Intake.select :close @17:09:09.90 SSV.dial :exhaust @17:09:10.66 Exhaust.select :open @17:09:10.77 SS.seek 0ml @17:09:19.75 Exhaust.select :close @17:09:19.86 SSV.dial CBV @17:09:19.88
CBV.dial SSV @17:09:21.05 Allowing 130s for 25.0ml of remaining 650.0ml @17:09:21.06 Intake.select :open @17:09:23.23 Intake @15.2psia, vacuum from 9.7 to 12.7psi @17:09:23.25 SS.reconfigure :inhaling 0.73ml/s @17:09:23.30 SS.seek 25ml @17:09:53.27 Vacuum too high -- waiting up to 40 seconds @17:09:53.29 SS.reconfigure :exhale @17:09:53.34 SS.hold @17:10:06.14 Intake @15.3psia, vacuum from 9.8 to 12.8psi @17:10:06.16 SS.reconfigure :inhaling 0.59ml/s @17:10:06.21 SS.seek 25ml @17:10:13.93 Stroke completed -- waiting for pressure to equalize ... @17:10:13.95 SS.reconfigure :exhale @17:10:13.99 SS.hold @17:10:27.09 Intake.select :close @17:10:27.25 SSV.dial :exhaust @17:10:28.00 Exhaust.select :open @17:10:28.12 SS.seek 0ml @17:10:37.09 Exhaust.select :close @17:10:37.21 SSV.dial CBV @17:10:37.24
CBV.dial SSV @17:10:37.99 Allowing 130s for 25.0ml of remaining 625.0ml @17:10:38.00 Intake.select :open @17:10:40.17 Intake @15.2psia, vacuum from 9.7 to 12.7psi @17:10:40.19 SS.reconfigure :inhaling 0.59ml/s @17:10:40.24 SS.seek 25ml @17:11:24.86 Stroke completed -- waiting for pressure to equalize ... @17:11:24.87 SS.reconfigure :exhale @17:11:24.91 SS.hold @17:11:49.87 Intake.select :close @17:11:50.02 SSV.dial :exhaust @17:11:50.78 Exhaust.select :open @17:11:50.89 SS.seek 0ml @17:11:59.90 Exhaust.select :close @17:12:00.01 SSV.dial CBV @17:12:00.04
CBV.dial SSV @17:12:00.80 Allowing 130s for 25.0ml of remaining 600.0ml @17:12:00.81 Intake.select :open @17:12:02.97 Intake @15.1psia, vacuum from 9.6 to 12.6psi @17:12:02.99 SS.reconfigure :inhaling 0.59ml/s @17:12:03.04 SS.seek 25ml @17:12:41.74 Vacuum too high -- waiting up to 40 seconds @17:12:41.75 SS.reconfigure :exhale @17:12:41.80 SS.hold @17:12:56.74 Intake @15.2psia, vacuum from 9.7 to 12.7psi @17:12:56.76 SS.reconfigure :inhaling 0.47ml/s @17:12:56.81 SS.seek 25ml @17:13:04.46 Stroke completed -- waiting for pressure to equalize ... @17:13:04.47 SS.reconfigure :exhale @17:13:04.52 SS.hold @17:13:20.83 Intake.select :close @17:13:20.98 SSV.dial :exhaust @17:13:21.75 Exhaust.select :open @17:13:21.86 SS.seek 0ml @17:13:30.82 Exhaust.select :close @17:13:30.93 SSV.dial CBV @17:13:30.96
CBV.dial SSV @17:13:31.72 Allowing 130s for 25.0ml of remaining 575.0ml @17:13:31.73 Intake.select :open @17:13:33.89 Intake @15.1psia, vacuum from 9.6 to 12.6psi @17:13:33.91 SS.reconfigure :inhaling 0.47ml/s @17:13:33.96 SS.seek 25ml @17:14:29.27 Stroke completed -- waiting for pressure to equalize ... @17:14:29.28 SS.reconfigure :exhale @17:14:29.33 SS.hold @17:15:02.16 Intake.select :close @17:15:02.32 SSV.dial :exhaust @17:15:03.07 Exhaust.select :open @17:15:03.19 SS.seek 0ml @17:15:11.99 Exhaust.select :close @17:15:12.10 SSV.dial CBV @17:15:12.13
CBV.dial SSV @17:15:12.89 Allowing 130s for 25.0ml of remaining 550.0ml @17:15:12.90 Intake.select :open @17:15:15.07 Intake @15.1psia, vacuum from 9.6 to 12.6psi @17:15:15.08 SS.reconfigure :inhaling 0.47ml/s @17:15:15.13 SS.seek 25ml @17:15:58.77 Vacuum too high -- waiting up to 40 seconds @17:15:58.79 SS.reconfigure :exhale @17:15:58.84 SS.hold @17:16:17.29 Intake @15.2psia, vacuum from 9.7 to 12.7psi @17:16:17.31 SS.reconfigure :inhaling 0.38ml/s @17:16:17.36 SS.seek 25ml @17:16:32.93 Stroke completed -- waiting for pressure to equalize ... @17:16:32.94 SS.reconfigure :exhale @17:16:32.98 SS.hold @17:16:55.43 Intake.select :close @17:16:55.59 SSV.dial :exhaust @17:16:56.34 Exhaust.select :open @17:16:56.45 SS.seek 0ml @17:17:05.48 Exhaust.select :close @17:17:05.59 SSV.dial CBV @17:17:05.61
CBV.dial SSV @17:17:06.37 Allowing 130s for 25.0ml of remaining 525.0ml @17:17:06.38 Intake.select :open @17:17:08.55 Intake @15.1psia, vacuum from 9.6 to 12.6psi @17:17:08.56 SS.reconfigure :inhaling 0.38ml/s @17:17:08.61 SS.seek 25ml @17:18:19.52 Stroke completed -- waiting for pressure to equalize ... @17:18:19.53 SS.reconfigure :exhale @17:18:19.58 SS.hold @17:18:58.40 Intake.select :close @17:18:58.56 SSV.dial :exhaust @17:18:59.33 Exhaust.select :open @17:18:59.44 SS.seek 0ml @17:19:08.22 Exhaust.select :close @17:19:08.34 SSV.dial CBV @17:19:08.36
CBV.dial SSV @17:19:09.12 Allowing 130s for 25.0ml of remaining 500.0ml @17:19:09.13 Intake.select :open @17:19:11.29 Intake @15.1psia, vacuum from 9.6 to 12.6psi @17:19:11.31 SS.reconfigure :inhaling 0.38ml/s @17:19:11.36 SS.seek 25ml @17:20:09.60 Vacuum too high -- waiting up to 40 seconds @17:20:09.62 SS.reconfigure :exhale @17:20:09.67 SS.hold @17:20:30.63 Intake @15.3psia, vacuum from 9.8 to 12.8psi @17:20:30.65 SS.reconfigure :inhaling 0.31ml/s @17:20:30.69 SS.seek 25ml @17:20:46.88 Stroke completed -- waiting for pressure to equalize ... @17:20:46.89 SS.reconfigure :exhale @17:20:46.93 SS.hold @17:21:13.90 Intake.select :close @17:21:14.06 SSV.dial :exhaust @17:21:14.82 Exhaust.select :open @17:21:14.94 SS.seek 0ml @17:21:24.03 Exhaust.select :close @17:21:24.14 SSV.dial CBV @17:21:24.17
CBV.dial SSV @17:21:24.92 Allowing 130s for 25.0ml of remaining 475.0ml @17:21:24.94 Intake.select :open @17:21:27.10 Intake @15.1psia, vacuum from 9.6 to 12.6psi @17:21:27.12 SS.reconfigure :inhaling 0.31ml/s @17:21:27.17 SS.seek 25ml @17:22:59.79 Stroke completed -- waiting for pressure to equalize ... @17:22:59.81 SS.reconfigure :exhale @17:22:59.85 SS.hold @17:23:34.98 SS.reconfigure :default @17:23:35.03 SS.hold @17:23:35.10 Intake.select :close @17:23:35.25 ShallowSampler::Clogged in MAIN -- Filtering too Slowly after 550.0ml @17:23:35.26 SSV.dial :exhaust @17:23:36.03 Exhaust.select :open @17:23:36.14 SS.reconfigure :exhale @17:23:36.19 SS.seek 0ml @17:23:45.04 Exhaust.select :close @17:23:45.15 Sampled 550.0ml @17:23:45.17 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @17:23:45.18 Intake.select :close @17:23:45.25 CSV.dial :waste @17:23:45.41 SSV.dial CBV,avoiding: :exhaust @17:23:45.44 CS.seek 0ml @17:23:45.47
CBV.dial SSV @17:23:45.57 CSV.dial :air @17:23:46.31 CS.seek 10ml @17:23:47.35
CTV.dial :air @17:23:48.12 delay 15 seconds @17:24:03.14 SSV.dial :spit,avoiding: :exhaust @17:24:04.46 CTV.dial CSV,avoiding: :intake @17:24:04.50
SS.seek 0ml @17:24:04.62 SSV.dial CBV,avoiding: :exhaust @17:24:10.85 SSV.dial between CBV and plug @17:24:11.71 CBV.dial between SSV and waste2 @17:24:12.18 CTV.dial CSV,avoiding: :air @17:24:12.21
CSV.dial CTV @17:24:12.86 CS.reconfigure :pressurize @17:24:12.91 CS.seek 0ml @17:24:31.21 BubblePoint: Intake @24.1psia, Outlet @0.5psia @17:24:31.22 CBV.dial SSV @17:24:31.56 SS.reconfigure :default @17:24:31.61 SS.seek 10ml @17:24:37.27 delay 15 seconds @17:24:52.40 Evacuated: Intake @23.7psia, Outlet @23.9psia @17:24:52.41 SSV.dial :spit,avoiding: :exhaust @17:24:53.46 SS.reconfigure :exhale @17:24:53.50 SS.seek 0ml @17:24:58.50 SS.reconfigure :fast @17:24:58.55 SS.seek 5ml @17:25:01.38 SSV.dial CBV,avoiding: :exhaust @17:25:01.41
CBV.dial SSV @17:25:02.75 CTV.dial :air,avoiding: :intake @17:25:04.07 CSV.dial CTV @17:25:04.11
SS.seek 10ml @17:25:05.23 CSV.dial :air @17:25:05.67 CS.reconfigure :fast @17:25:05.72 CS.seek 0ml @17:25:06.61
SSV.dial :spit,avoiding: :exhaust @17:25:07.93 SS.reconfigure :exhale @17:25:07.97 SS.seek 0ml @17:25:13.04 SS.reconfigure :fast @17:25:13.09 SS.seek 5ml @17:25:13.60 CS.stop @17:25:15.92 SSV.dial CBV,avoiding: :exhaust @17:25:15.95
CBV.dial SSV @17:25:18.30 SSV.dial :spit,avoiding: :exhaust @17:25:19.61 SS.reconfigure :default @17:25:19.66 SS.seek 0ml @17:25:22.92 SS.stop @17:25:22.96 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @17:25:22.97 Intake.select :close @17:25:23.04 CSV.dial :waste @17:25:23.18 SSV.dial CBV,avoiding: :exhaust @17:25:23.20
CBV.dial SSV @17:25:23.81 CS.seek 0ml @17:25:23.88 CSV.dial :air @17:25:24.56
CTV.dial :air @17:25:24.64 CS.seek 10ml @17:25:24.73
delay 15 seconds @17:25:39.74 SSV.dial :spit,avoiding: :exhaust @17:25:41.13 CTV.dial CSV,avoiding: :intake @17:25:41.17
SS.reconfigure :exhale @17:25:41.27 SS.seek 0ml @17:25:41.67 SSV.dial CBV,avoiding: :exhaust @17:25:49.35 SSV.dial between CBV and plug @17:25:49.83 CBV.dial between SSV and waste2 @17:25:50.24 CSV.dial between air and CTV @17:25:50.55 CS.seek 7.357ml @17:25:57.35 CTV.dial CSV,avoiding: :air @17:25:57.38
CSV.dial CTV @17:25:57.71 CS.reconfigure :pressurize @17:25:57.77 CS.seek 0ml @17:26:10.67 BubblePoint: Intake @24.3psia, Outlet @4.1psia @17:26:10.68 CBV.dial SSV @17:26:11.01 SS.reconfigure :default @17:26:11.06 SS.seek 10ml @17:26:16.64 delay 15 seconds @17:26:31.77 Evacuated: Intake @24.0psia, Outlet @24.1psia @17:26:31.78 SSV.dial :spit,avoiding: :exhaust @17:26:32.87 SS.reconfigure :exhale @17:26:32.91 SS.seek 0ml @17:26:38.21 SS.reconfigure :fast @17:26:38.26 SS.seek 5ml @17:26:40.88 SSV.dial CBV,avoiding: :exhaust @17:26:40.90
CBV.dial SSV @17:26:42.26 CTV.dial :air,avoiding: :intake @17:26:43.60 CSV.dial CTV @17:26:43.64
SS.seek 10ml @17:26:44.84 CSV.dial :air @17:26:45.27 CS.reconfigure :fast @17:26:45.32 CS.seek 0ml @17:26:46.12
SSV.dial :spit,avoiding: :exhaust @17:26:47.44 SS.reconfigure :exhale @17:26:47.49 SS.seek 0ml @17:26:51.88 CS.stop @17:26:52.68
SS.reconfigure :fast @17:26:52.72 SS.seek 5ml @17:26:55.69 SSV.dial CBV,avoiding: :exhaust @17:26:55.72
CBV.dial SSV @17:26:58.04 SSV.dial :spit,avoiding: :exhaust @17:26:59.36 SS.reconfigure :default @17:26:59.41 SS.seek 0ml @17:27:02.50 SS.stop @17:27:02.54 Lysing: Adding 2.0ml AqMeOH @17:27:02.55 CBV.dial between CSV and waste1,avoiding: :DBV @17:27:04.46 CTV.dial CSV @17:27:05.77 CSV.dial CSR @17:27:06.56 Pulling 1ml of CSR.AqMeOH @17:27:06.57 CSR.select :AqMeOH @17:27:06.69 CS.reconfigure :default @17:27:06.73 CS.seek 1ml @17:27:10.41 delay 5 seconds @17:27:15.43 CSR.select :air @17:27:15.60 CS.seek 6ml @17:27:29.22 delay 2 seconds @17:27:31.24 CSV.dial :waste2 @17:27:31.68 CS.reconfigure :fast @17:27:31.73 CS.seek 0ml @17:27:46.22 CSV.dial CSR @17:27:46.68 Pulling 2ml of CSR.AqMeOH CSR.select :AqMeOH @17:27:46.80 CS.reconfigure :default @17:27:46.85 CS.seek 2ml @17:27:53.00 delay 5 seconds @17:27:58.03 CSR.select :air @17:27:58.19 CS.seek 5ml @17:28:06.69 delay 2 seconds @17:28:08.71 CSV.dial CTV @17:28:09.15 CS.seek 1ml @17:28:20.36 Before lysing Collection Puck Heater at 18.1C/30.1psia @17:28:20.38 Heating Puck.from 2, :sh1 in Collection Clamp to 70C for 10:00 @17:28:20.40 CH.seek 70C,8:00 @17:30:26.43 CH.hold 10:00,70C @17:30:26.63 Begun lysing Collection Puck Heater at 68C/36.7psia @17:40:26.65 Ended lysing Collection Puck Heater at 70C/35.6psia @17:40:26.67 CS.seek 0ml @17:40:30.06 CTV.dial between spare and air,avoiding: :intake @17:40:31.09 CSV.dial :air @17:40:31.53 CS.seek 5ml @17:40:45.11 CSV.dial CTV @17:40:45.57 delay 2 seconds @17:40:47.59 CSV.dial :air @17:40:48.04 delay 2 seconds @17:40:50.05 CSV.dial CTV @17:40:50.51 delay 2 seconds @17:40:52.53 CSV.dial :air @17:40:52.98 delay 2 seconds @17:40:55.00 CS.seek 0ml @17:41:08.67 Puck @82.8psia after final lysis pressurization @17:41:08.68 Recovering Lysate into Collection syringe @17:41:08.69 CSV.dial CBV @17:41:09.71 CBV.dial CSV @17:41:10.29 CS.seek 5ml @17:41:24.08 delay 5 seconds @17:41:29.10 CTV.dial :air @17:41:29.63 CS.seek 7.001ml @17:41:35.90 delay 5 seconds @17:41:40.92 CSV.dial :air2 @17:41:41.37 CS.seek 10ml @17:41:49.80 CSV.dial between air and CTV,avoiding: CBV @17:41:50.95 Lysing Complete @17:41:51.01 Storing sh1 puck in Collection Clamp to tube 1 @17:41:51.19 SC.reconfigure :tight @17:41:51.24 SC.seek 1 @17:41:54.78 SC.configure :limp @17:41:55.01 Elbow.reconfigure :tight @17:41:55.05 Elbow.seek 1 @17:41:56.84 Forearm.reconfigure :tight @17:41:56.89 Forearm.seek 1 + 450 counts @17:41:57.94 SE.reconfigure :pick @17:41:58.00 SE.seek :beamBreak @17:41:59.81 SE.configure :default @17:41:59.99 Elbow.reconfigure :limp @17:42:00.04 Forearm.reconfigure :loose @17:42:00.08 Forearm.seek 1 @17:42:00.30 Elbow.hold @17:42:00.36 Elbow.reconfigure :tight @17:42:00.46 SE.seek 19.06 Pucks below Top Plate @17:42:00.90 Forearm.force -20 @17:42:00.95 Forearm.seek 1 + 308 counts @17:42:01.25 Forearm.reconfigure :tight @17:42:01.30 Forearm.seek 1 + 450 counts @17:42:01.52 SE.seek 20.06 Pucks below Top Plate @17:42:02.11 Forearm.reconfigure :loose @17:42:02.15 Forearm.seek :retract @17:42:03.09 Elbow.seek CC @17:42:04.78 Elbow.reconfigure :loose @17:42:04.89 Forearm.seek CC @17:42:05.87 Hand.close @17:42:06.52 CC.seek :open @17:42:11.26 Forearm.seek :retract @17:42:12.22 Elbow.reconfigure :tight @17:42:12.27 Elbow.seek 1 @17:42:14.02 Elbow.reconfigure :limp @17:42:14.07 Forearm.seek 1 @17:42:15.11 Elbow.hold @17:42:15.18 Elbow.reconfigure :tight @17:42:15.22 Hand.open @17:42:16.01 Forearm.force -20 @17:42:16.07 Forearm.seek 1 + 320 counts @17:42:16.44 Forearm.reconfigure :tight @17:42:16.48 Forearm.seek 1 + 450 counts @17:42:16.74 SE.seek :down @17:42:17.88
Forearm.reconfigure :loose @17:42:17.92 Forearm.seek :retract @17:42:18.06 SE.stop @17:42:18.86
Tranferring DA @17:42:18.87 Beginning samplePassC @17:42:18.89 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @17:42:19.08 Elbow.seek FlushPuck::Garage @17:42:20.94 Elbow.reconfigure :loose @17:42:21.06 Forearm.seek :garage @17:42:22.02 Hand.close @17:42:23.21 Forearm.seek :retract @17:42:24.19 Elbow.reconfigure :tight @17:42:24.24 Elbow.seek CC @17:42:27.20 Elbow.reconfigure :loose @17:42:27.78 Forearm.reconfigure :tight @17:42:27.83 Forearm.seek CC @17:42:29.10 CC.seek :closed @17:42:33.64 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.39 @17:42:33.71 Hand.open @17:42:34.33 Forearm.reconfigure :loose @17:42:34.37 Forearm.seek :retract @17:42:35.34 PSV.dial PSR @17:42:36.72 Pulling 1.5ml of PSR.flush @17:42:36.73 PSR.select :flush @17:42:36.85 PS.seek 1.5ml @17:42:53.06 delay 5 seconds @17:42:58.08 PSV.dial :waste1 @17:42:58.55 PS.seek 0ml @17:43:14.65 PSV.dial PSR @17:43:15.12 PSR.select :air @17:43:15.24 PS.seek 2.5ml @17:43:41.81 PRV.dial CBV,avoiding: :ab2 @17:43:41.84
CBV.dial PRV @17:43:43.57 PSV.dial :mixloop @17:43:44.73 CTV.dial :air,avoiding: :intake @17:43:44.80 PTV.dial :PRVmixing @17:43:46.11 PS.seek 0ml @17:44:12.49 PSV.dial :air @17:44:13.54 PS.seek 2.5ml @17:44:39.99 PSV.dial :mixloop @17:44:40.45 PS.seek 0ml @17:45:06.74 PSV.dial :air @17:45:07.22 PS.seek 2.5ml @17:45:33.52 PSV.dial :mixloop @17:45:34.00 PS.seek 0ml @17:46:00.37 Garaging FlushPuck in Collection Clamp @17:46:00.44 Elbow.reconfigure :tight @17:46:00.49 Elbow.seek CC @17:46:00.74 Elbow.reconfigure :loose @17:46:00.85 Forearm.seek CC @17:46:01.87 Hand.close @17:46:02.52 CC.seek :open @17:46:07.14 Forearm.seek :retract @17:46:08.11 Elbow.reconfigure :tight @17:46:08.16 Elbow.seek :garage @17:46:11.08 Elbow.reconfigure :loose @17:46:11.19 Forearm.reconfigure :tight @17:46:11.24 Forearm.seek :garage @17:46:12.18 Hand.open @17:46:12.83 Forearm.reconfigure :loose @17:46:12.87 Forearm.seek :retract @17:46:13.87 PSV.dial :mixloop @17:46:13.94 PTV.dial :PRVmixing @17:46:13.96 PRV.dial CBV @17:46:13.98
CBV.dial PRV @17:46:14.07 CTV.dial :air,avoiding: :intake @17:46:14.15 Loading lyfil puck from tube 2 into Collection Clamp @17:46:14.39 SC.reconfigure :tight @17:46:14.44 SC.seek 2 @17:46:18.00 SC.configure :limp @17:46:18.22 Elbow.reconfigure :tight @17:46:18.27 Elbow.seek 2 @17:46:20.62 Forearm.reconfigure :tight @17:46:20.67 Forearm.seek 2 + 450 counts @17:46:21.70 SE.reconfigure :pick @17:46:21.76 SE.seek :beamBreak @17:46:24.08 SE.configure :default @17:46:24.24 Elbow.seek 2 @17:46:24.43 Forearm.seek 2 + 300 counts @17:46:24.64 Forearm.reconfigure :loose @17:46:24.69 Hand.close @17:46:25.28 SE.seek :down @17:46:25.49
Forearm.seek :retract @17:46:26.48 Elbow.seek CC @17:46:27.35 SE.stop @17:46:28.10
Elbow.reconfigure :loose @17:46:28.21 Forearm.reconfigure :tight @17:46:28.26 Forearm.seek CC @17:46:29.50 CC.seek :closed @17:46:34.02 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.14 @17:46:34.04 Hand.open @17:46:34.71 Forearm.reconfigure :loose @17:46:34.75 Forearm.seek :retract @17:46:35.70 CTV.dial CSV,avoiding: :intake @17:46:37.49 CSV.dial CTV @17:46:37.82 PS.seek 2.5ml @17:47:04.30 CS.seek 4.5ml @17:47:19.24 delay 8 seconds @17:47:27.26 PSV.dial :air @17:47:27.73 delay 2 seconds @17:47:29.74 PSV.dial :waste2 @17:47:30.48 PS.seek 0ml @17:47:56.86 CBV.dial between PRV and waste1 @17:47:57.19 CSV.dial :air @17:47:57.77 CS.seek 10ml @17:48:12.86 CSV.dial CTV @17:48:13.31 CS.seek 0ml @17:48:39.73 CBV.dial :waste1 @17:48:40.05 delay 5 seconds @17:48:45.07 CTV.dial :air,avoiding: :intake @17:48:46.46 Storing lyfil puck in Collection Clamp to tube 1 @17:48:46.64 SC.reconfigure :tight @17:48:46.69 SC.seek 1 @17:48:50.35 SC.configure :limp @17:48:50.57 Elbow.reconfigure :tight @17:48:50.62 Elbow.seek 1 @17:48:52.40 Forearm.reconfigure :tight @17:48:52.45 Forearm.seek 1 + 450 counts @17:48:53.46 SE.reconfigure :pick @17:48:53.52 SE.seek :beamBreak @17:48:55.21 SE.configure :default @17:48:55.38 Elbow.reconfigure :limp @17:48:55.43 Forearm.reconfigure :loose @17:48:55.48 Forearm.seek 1 @17:48:55.78 Elbow.hold @17:48:55.84 Elbow.reconfigure :tight @17:48:55.94 SE.seek 20.05 Pucks below Top Plate @17:48:56.90 Forearm.force -20 @17:48:56.94 Forearm.seek 1 + 305 counts @17:48:57.26 Forearm.reconfigure :tight @17:48:57.31 Forearm.seek 1 + 450 counts @17:48:57.52 SE.seek 21.05 Pucks below Top Plate @17:48:58.10 Forearm.reconfigure :loose @17:48:58.15 Forearm.seek :retract @17:48:59.11 Elbow.seek CC @17:49:00.78 Elbow.reconfigure :loose @17:49:00.88 Forearm.seek CC @17:49:01.87 Hand.close @17:49:02.51 CC.seek :open @17:49:07.29 Forearm.seek :retract @17:49:08.29 Elbow.reconfigure :tight @17:49:08.34 Elbow.seek 1 @17:49:10.14 Elbow.reconfigure :limp @17:49:10.20 Forearm.seek 1 @17:49:11.20 Elbow.hold @17:49:11.26 Elbow.reconfigure :tight @17:49:11.31 Hand.open @17:49:12.01 Forearm.force -20 @17:49:12.07 Forearm.seek 1 + 309 counts @17:49:12.45 Forearm.reconfigure :tight @17:49:12.50 Forearm.seek 1 + 450 counts @17:49:12.81 SE.seek :down @17:49:14.01
Forearm.reconfigure :loose @17:49:14.06 Forearm.seek :retract @17:49:14.11 SE.stop @17:49:15.03
PSV.dial :air @17:49:15.78 PS.seek 2.5ml @17:49:42.17 delay 1 second @17:49:43.19 PSV.dial :mixloop @17:49:43.65 delay 1 second @17:49:44.66 PSV.dial :air @17:49:45.25 delay 1 second @17:49:46.27 PSV.dial :mixloop @17:49:46.72 delay 1 second @17:49:47.74 PSV.dial :air @17:49:48.21 delay 1 second @17:49:49.23 PS.seek 0ml @17:50:15.55 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @17:50:15.80 Elbow.seek FlushPuck::Garage @17:50:17.61 Elbow.reconfigure :loose @17:50:17.72 Forearm.seek :garage @17:50:18.67 Hand.close @17:50:19.38 Forearm.seek :retract @17:50:20.36 Elbow.reconfigure :tight @17:50:20.41 Elbow.seek PC @17:50:21.66 Elbow.reconfigure :loose @17:50:21.77 Forearm.reconfigure :tight @17:50:21.81 Forearm.seek PC @17:50:23.12 PC.seek :closed @17:50:27.74 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.61 @17:50:28.25 Hand.open @17:50:28.95 Forearm.reconfigure :loose @17:50:29.00 Forearm.seek :retract @17:50:29.95 PRV.dial :air @17:50:30.40 PSV.dial :mixloop @17:50:30.93 PS.seek 0.3ml @17:50:35.02 PSV.dial PSR @17:50:36.06 Pulling 0.4ml of PSR.air @17:50:36.07 PSR.select :air @17:50:36.10 PS.seek 0.7ml @17:50:41.06 delay 3 seconds @17:50:44.07 PSR.select :air @17:50:44.10 PS.seek 2.5ml @17:51:03.36 delay 3 seconds @17:51:06.38 PSV.dial :mixloop @17:51:07.43 PTV.dial :mixing @17:51:08.17 PBV.dial :waste2 @17:51:09.13 PS.seek 0ml @17:51:35.52 Garaging FlushPuck in Processing Clamp @17:51:35.59 Elbow.reconfigure :tight @17:51:35.64 Elbow.seek PC @17:51:35.76 Elbow.reconfigure :loose @17:51:35.87 Forearm.seek PC @17:51:36.80 Hand.close @17:51:37.39 PC.seek :open @17:51:42.14 Forearm.seek :retract @17:51:43.10 Elbow.reconfigure :tight @17:51:43.15 Elbow.seek :garage @17:51:44.52 Elbow.reconfigure :loose @17:51:44.62 Forearm.reconfigure :tight @17:51:44.67 Forearm.seek :garage @17:51:45.74 Hand.open @17:51:46.45 Forearm.reconfigure :loose @17:51:46.50 Forearm.seek :retract @17:51:47.51 Loading da puck from tube 2 into Processing Clamp @17:51:47.80 SC.reconfigure :tight @17:51:47.85 SC.seek 2 @17:51:51.47 SC.configure :limp @17:51:51.70 Elbow.reconfigure :tight @17:51:51.74 Elbow.seek 2 @17:51:53.60 Forearm.reconfigure :tight @17:51:53.65 Forearm.seek 2 + 450 counts @17:51:54.73 SE.reconfigure :pick @17:51:54.79 SE.seek :beamBreak @17:51:57.20 SE.configure :default @17:51:57.35 Elbow.seek 2 @17:51:57.54 Forearm.seek 2 + 300 counts @17:51:57.77 Forearm.reconfigure :loose @17:51:57.82 Hand.close @17:51:58.40 SE.seek :down @17:51:58.62
Forearm.seek :retract @17:51:59.60 Elbow.seek PC @17:52:00.52 SE.stop @17:52:02.14
Elbow.reconfigure :loose @17:52:02.25 Forearm.reconfigure :tight @17:52:02.29 Forearm.seek PC @17:52:03.53 PC.seek :closed @17:52:08.20 Processing Clamp onSpring/offSpring for 16/26 samples, on/off torque 1.62 @17:52:08.21 Hand.open @17:52:08.95 Forearm.reconfigure :loose @17:52:09.00 Forearm.seek :retract @17:52:10.45 Heating Puck.from 2, :da in Processing Clamp to 28C for 2:00:00 @17:52:10.46 PH.seek 28C,5:00 @17:52:47.49 PH.hold 2:00:00,28C @17:52:47.54 delay 1:30 @17:54:17.58 PSR.select :air @17:54:17.59 PSV.dial PSR @17:54:18.64 Pulling 0.5ml of PSR.wash @17:54:18.65 PSR.select :wash @17:54:18.77 PS.seek 0.5ml @17:54:24.64 delay 5 seconds @17:54:29.66 PSR.select :air @17:54:29.78 PS.seek 2ml @17:54:45.89 PTV.dial :puck @17:54:46.62 PSV.dial PTV @17:54:47.39 PBV.dial :waste1 @17:54:48.78 PS.seek 1.5ml @17:54:54.73 delay 3 seconds @17:54:57.75 PBV.dial between PSV and waste1 @17:54:58.10 PS.seek 0ml @17:55:14.71 delay 5:00 @18:00:14.73 recoverPSRreagent08 @18:00:14.74 PTV.dial :puck @18:00:14.75 PBV.dial :waste1 @18:00:15.12 delay 30 seconds @18:00:45.14 PBV.dial between PSV and waste1 @18:00:45.51 PSR.select :air @18:00:45.52 PSV.dial PSR @18:00:46.28 PS.reconfigure :fast @18:00:46.33 PS.seek 2.5ml @18:01:10.25 PSV.dial PTV @18:01:11.01 delay 5 seconds @18:01:16.03 PSV.dial PSR @18:01:16.79 delay 5 seconds @18:01:21.81 PSV.dial PTV @18:01:22.57 delay 5 seconds @18:01:27.59 PSV.dial :air @18:01:28.46 PS.seek 0ml @18:01:52.16 PTV.dial :PRVmixing,via: PRV @18:01:53.47 PSV.dial :mixloop @18:01:53.93 PRV.dial :ab1 @18:01:54.68 PS.reconfigure :slow @18:01:54.73 PS.seek 0.15ml @18:01:58.29 delay 2 seconds @18:02:00.30 CBV.dial PRV @18:02:00.33
PRV.dial CBV @18:02:00.90 PS.seek 0.35ml @18:02:05.44 delay 2 seconds @18:02:07.46 CBV.dial between PRV and PSV @18:02:07.77 PRV.dial :air @18:02:08.23 PS.seek 0.9ml @18:02:19.91 PRV.dial :waste2 @18:02:20.65 PS.seek 0.5ml @18:02:29.29 addPMLReagent08 PSV.dial :air @18:02:29.76 PS.reconfigure :default @18:02:29.86 PS.seek 2ml @18:02:46.09 PSV.dial :PML @18:02:46.57 PTV.dial :mixing @18:02:47.33 PBV.dial :waste1 @18:02:47.75 PS.seek 1.85ml @18:02:50.03 PBV.dial between PSV and waste1 @18:02:50.40 PS.seek 0ml @18:03:10.13 delay 2 seconds @18:03:12.15 Beginning samplePassC @18:03:12.16 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @18:03:12.35 Elbow.reconfigure :tight @18:03:12.39 Elbow.seek FlushPuck::Garage @18:03:14.19 Elbow.reconfigure :loose @18:03:14.30 Forearm.seek :garage @18:03:15.29 Hand.close @18:03:16.00 Forearm.seek :retract @18:03:16.97 Elbow.reconfigure :tight @18:03:17.02 Elbow.seek CC @18:03:19.90 Elbow.reconfigure :loose @18:03:20.01 Forearm.reconfigure :tight @18:03:20.05 Forearm.seek CC @18:03:21.28 CC.seek :closed @18:03:25.82 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.43 @18:03:25.89 Hand.open @18:03:26.56 Forearm.reconfigure :loose @18:03:26.61 Forearm.seek :retract @18:03:27.56 PSV.dial PSR @18:03:28.61 Pulling 1.5ml of PSR.flush @18:03:28.62 PSR.select :flush @18:03:28.74 PS.seek 1.5ml @18:03:44.97 delay 5 seconds @18:03:49.99 PSV.dial :waste1 @18:03:50.46 PS.seek 0ml @18:04:06.56 PSV.dial PSR @18:04:07.03 PSR.select :air @18:04:07.15 PS.seek 2.5ml @18:04:33.54 PRV.dial CBV,avoiding: :ab2 @18:04:33.56
CBV.dial PRV @18:04:35.19 PSV.dial :mixloop @18:04:36.22 CTV.dial :air,avoiding: :intake @18:04:36.75 PTV.dial :PRVmixing @18:04:37.52 PS.seek 0ml @18:05:03.87 PSV.dial :air @18:05:04.35 PS.seek 2.5ml @18:05:30.78 PSV.dial :mixloop @18:05:31.26 PS.seek 0ml @18:05:57.59 PSV.dial :air @18:05:58.07 PS.seek 2.5ml @18:06:24.44 PSV.dial :mixloop @18:06:24.91 PS.seek 0ml @18:06:51.31 Garaging FlushPuck in Collection Clamp @18:06:51.39 Elbow.reconfigure :tight @18:06:51.43 Elbow.seek CC @18:06:51.71 Elbow.reconfigure :loose @18:06:51.81 Forearm.seek CC @18:06:52.87 Hand.close @18:06:53.50 CC.seek :open @18:06:58.12 Forearm.seek :retract @18:06:59.08 Elbow.reconfigure :tight @18:06:59.13 Elbow.seek :garage @18:07:02.02 Elbow.reconfigure :loose @18:07:02.12 Forearm.reconfigure :tight @18:07:02.17 Forearm.seek :garage @18:07:03.18 Hand.open @18:07:03.81 Forearm.reconfigure :loose @18:07:03.86 Forearm.seek :retract @18:07:04.87 delayUntil "18:11:12.13" @18:07:04.90 Whole Cell Archive sampling 1000ml @18:07:04.92 Loading wcr puck from tube 2 into Collection Clamp @18:07:05.23 SC.reconfigure :tight @18:07:05.28 SC.seek 2 @18:07:05.55 SC.configure :limp @18:07:06.22 Elbow.reconfigure :tight @18:07:06.28 Elbow.seek 2 @18:07:08.21 Forearm.reconfigure :tight @18:07:08.26 Forearm.seek 2 + 450 counts @18:07:09.30 SE.reconfigure :pick @18:07:09.36 SE.seek :beamBreak @18:07:11.81 SE.configure :default @18:07:11.98 Elbow.seek 2 @18:07:12.23 Forearm.seek 2 + 300 counts @18:07:12.43 Forearm.reconfigure :loose @18:07:12.48 Hand.close @18:07:13.13 SE.seek :down @18:07:13.34 Forearm.seek :retract @18:07:14.26 Elbow.seek CC @18:07:15.36 SE.stop @18:07:15.90 Elbow.reconfigure :loose @18:07:16.01 Forearm.reconfigure :tight @18:07:16.06 Forearm.seek CC @18:07:17.28 CC.seek :closed @18:07:21.80 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.28 @18:07:21.82 Hand.open @18:07:23.00 Forearm.reconfigure :loose @18:07:23.05 Forearm.seek :retract @18:07:24.01 collectSample 1000ml w/bubble point of 35psi @18:07:24.03 Priming 7ml @18:07:24.04 Intake.select :close @18:07:24.05 Exhaust.select :close @18:07:24.06 SS.hold @18:07:24.28 Calibrating pressure sensors @18:07:24.29 CTV.dial :air @18:07:24.37 SSV.dial :spit,avoiding: :exhaust @18:07:24.47 SS.reconfigure :fast @18:07:24.52 SS.seek 5ml @18:07:27.12 SSV.dial CBV @18:07:27.15 CBV.dial SSV @18:07:28.51 delay 3.8 seconds @18:07:32.44 Sensor limits: Intake max 115.8psia; Outlet max 108.5psia @18:07:32.45 SSV.dial :spit @18:07:33.78 SS.seek 0ml @18:07:36.57 SSV.dial CBV @18:07:37.89 CTV.dial :intake @18:07:38.67 SSV.dial CBV @18:07:38.70 CBV.dial SSV @18:07:38.84 Allowing 40s for 7.0ml of remaining 7.0ml @18:07:38.86 Intake.select :open @18:07:41.03 Intake @14.7psia, vacuum from 9.2 to 12.2psi @18:07:41.05 SS.reconfigure :inhaling 2.20ml/s @18:07:41.09 SS.seek 7ml @18:07:47.33 Stroke completed -- waiting for pressure to equalize ... @18:07:47.35 SS.reconfigure :exhale @18:07:47.39 SS.hold @18:07:55.98 Intake.select :close @18:07:56.14 SSV.dial :exhaust @18:07:56.91 Exhaust.select :open @18:07:57.03 SS.seek 0ml @18:08:01.05 Exhaust.select :close @18:08:01.17 Sampling 1000.0ml @18:08:01.18 Intake.select :close @18:08:01.19 Exhaust.select :close @18:08:01.21 SS.reconfigure :default @18:08:01.25 SS.hold @18:08:01.47 CTV.dial :intake @18:08:01.69 SSV.dial CBV @18:08:01.71 CBV.dial SSV @18:08:02.51 Allowing 130s for 25.0ml of remaining 1000.0ml @18:08:02.52 Intake.select :open @18:08:04.69 Intake @15.3psia, vacuum from 9.8 to 12.8psi @18:08:04.71 SS.reconfigure :inhaling 2.20ml/s @18:08:04.76 SS.seek 25ml @18:08:16.70 Vacuum too high -- waiting up to 40 seconds @18:08:16.72 SS.reconfigure :exhale @18:08:16.77 SS.hold @18:08:25.08 Intake @15.9psia, vacuum from 10.0 to 13.0psi @18:08:25.10 SS.reconfigure :inhaling 1.76ml/s @18:08:25.15 SS.seek 25ml @18:08:29.63 Stroke completed -- waiting for pressure to equalize ... @18:08:29.64 SS.reconfigure :exhale @18:08:29.69 SS.hold @18:08:46.02 Intake.select :close @18:08:46.19 SSV.dial :exhaust @18:08:46.96 Exhaust.select :open @18:08:47.08 SS.seek 0ml @18:08:56.01 Exhaust.select :close @18:08:56.12 SSV.dial CBV @18:08:56.14 CBV.dial SSV @18:08:56.93 Allowing 130s for 25.0ml of remaining 975.0ml @18:08:56.94 Intake.select :open @18:08:59.11 Intake @15.6psia, vacuum from 10.0 to 13.0psi @18:08:59.13 SS.reconfigure :inhaling 1.76ml/s @18:08:59.18 SS.seek 25ml @18:09:15.01 Vacuum too high -- waiting up to 40 seconds @18:09:15.03 SS.reconfigure :exhale @18:09:15.09 SS.hold @18:09:23.93 Intake @16.8psia, vacuum from 10.0 to 13.0psi @18:09:23.95 SS.reconfigure :inhaling 1.41ml/s @18:09:24.00 SS.seek 25ml @18:09:25.71 Stroke completed -- waiting for pressure to equalize ... @18:09:26.17 SS.reconfigure :exhale @18:09:26.22 SS.hold @18:09:37.38 Intake.select :close @18:09:37.54 SSV.dial :exhaust @18:09:38.30 Exhaust.select :open @18:09:38.42 SS.seek 0ml @18:09:47.38 Exhaust.select :close @18:09:47.49 SSV.dial CBV @18:09:47.53 CBV.dial SSV @18:09:48.29 Allowing 130s for 25.0ml of remaining 950.0ml @18:09:48.30 Intake.select :open @18:09:50.46 Intake @15.7psia, vacuum from 10.0 to 13.0psi @18:09:50.48 SS.reconfigure :inhaling 1.41ml/s @18:09:50.53 SS.seek 25ml @18:10:10.78 Stroke completed -- waiting for pressure to equalize ... @18:10:10.80 SS.reconfigure :exhale @18:10:10.84 SS.hold @18:10:29.54 Intake.select :close @18:10:29.70 SSV.dial :exhaust @18:10:30.48 Exhaust.select :open @18:10:30.59 SS.seek 0ml @18:10:39.74 Exhaust.select :close @18:10:39.85 SSV.dial CBV @18:10:39.87 CBV.dial SSV @18:10:40.66 Allowing 130s for 25.0ml of remaining 925.0ml @18:10:40.68 Intake.select :open @18:10:43.22 Intake @15.6psia, vacuum from 10.0 to 13.0psi @18:10:43.31 SS.reconfigure :inhaling 1.41ml/s @18:10:43.36 SS.seek 25ml @18:11:03.13 Stroke completed -- waiting for pressure to equalize ... @18:11:03.14 SS.reconfigure :exhale @18:11:03.19 SS.hold @18:11:12.14 recoverPRVreagent08 @18:11:12.15 PTV.dial :mixing @18:11:12.95 PBV.dial :waste1 @18:11:13.37 delay 30 seconds @18:11:19.54 Intake.select :close @18:11:19.70 SSV.dial :exhaust @18:11:20.47 Exhaust.select :open @18:11:20.59 SS.seek 0ml @18:11:29.35 Exhaust.select :close @18:11:29.91 SSV.dial CBV @18:11:29.94 CBV.dial SSV @18:11:30.72 Allowing 130s for 25.0ml of remaining 900.0ml @18:11:30.74 Intake.select :open @18:11:32.91 Intake @15.8psia, vacuum from 10.0 to 13.0psi @18:11:32.93 SS.reconfigure :inhaling 1.41ml/s @18:11:32.98 SS.seek 25ml @18:11:43.39 PBV.dial between PSV and waste1 @18:11:43.77 PSV.dial :air @18:11:44.25 PS.reconfigure :fast @18:11:44.30 PS.seek 2.5ml @18:11:48.89 Vacuum too high -- waiting up to 40 seconds @18:11:48.91 SS.reconfigure :exhale @18:11:48.96 SS.hold @18:11:54.01 Intake @14.8psia, vacuum from 9.3 to 12.3psi @18:11:54.03 SS.reconfigure :inhaling 1.13ml/s @18:11:54.08 SS.seek 25ml @18:12:00.20 Stroke completed -- waiting for pressure to equalize ... @18:12:00.22 SS.reconfigure :exhale @18:12:00.26 SS.hold @18:12:08.36 PSV.dial :mixloop @18:12:08.84 delay 2 seconds @18:12:10.53 Intake.select :close @18:12:10.69 SSV.dial :exhaust @18:12:10.85 PSV.dial :air @18:12:11.32 delay 2 seconds @18:12:11.47 Exhaust.select :open @18:12:11.59 SS.seek 0ml @18:12:13.34 PSV.dial :mixloop @18:12:13.81 delay 2 seconds @18:12:15.82 PSV.dial :air @18:12:16.31 PS.seek 0ml @18:12:20.39 Exhaust.select :close @18:12:20.51 SSV.dial CBV @18:12:20.54 CBV.dial SSV @18:12:21.33 Allowing 130s for 25.0ml of remaining 875.0ml @18:12:21.35 Intake.select :open @18:12:23.51 Intake @15.7psia, vacuum from 10.0 to 13.0psi @18:12:23.53 SS.reconfigure :inhaling 1.13ml/s @18:12:23.58 SS.seek 25ml @18:12:40.18 washCycleDA #1 of 2 @18:12:40.19 PSR.select :air @18:12:40.21 PSV.dial PSR @18:12:41.54 Pulling 0.5ml of PSR.wash @18:12:41.55 PSR.select :wash @18:12:41.67 PS.reconfigure :default @18:12:41.72 PS.seek 0.5ml @18:12:47.68 delay 5 seconds @18:12:48.58 Stroke completed -- waiting for pressure to equalize ... @18:12:48.59 SS.reconfigure :exhale @18:12:48.64 SS.hold @18:12:52.69 PSR.select :air @18:12:52.82 PS.seek 2ml @18:13:01.62 Intake.select :close @18:13:01.78 SSV.dial :exhaust @18:13:02.55 Exhaust.select :open @18:13:02.66 SS.seek 0ml @18:13:08.96 PTV.dial :puck @18:13:09.71 PSV.dial PTV @18:13:10.46 PBV.dial :waste1 @18:13:10.90 PS.seek 1.5ml @18:13:11.70 Exhaust.select :close @18:13:11.82 SSV.dial CBV @18:13:11.86 CBV.dial SSV @18:13:12.61 Allowing 130s for 25.0ml of remaining 850.0ml @18:13:12.63 Intake.select :open @18:13:14.79 Intake @15.6psia, vacuum from 10.0 to 13.0psi @18:13:14.81 SS.reconfigure :inhaling 1.13ml/s @18:13:14.86 SS.seek 25ml @18:13:16.77 delay 3 seconds @18:13:19.79 PBV.dial between PSV and waste1 @18:13:20.19 PS.seek 0ml @18:13:36.30 delay 2:00 @18:13:39.55 Stroke completed -- waiting for pressure to equalize ... @18:13:39.56 SS.reconfigure :exhale @18:13:39.61 SS.hold @18:13:54.49 Intake.select :close @18:13:54.65 SSV.dial :exhaust @18:13:55.42 Exhaust.select :open @18:13:55.54 SS.seek 0ml @18:14:04.41 Exhaust.select :close @18:14:04.52 SSV.dial CBV @18:14:04.55 CBV.dial SSV @18:14:05.36 Allowing 130s for 25.0ml of remaining 825.0ml @18:14:05.38 Intake.select :open @18:14:07.55 Intake @15.5psia, vacuum from 10.0 to 13.0psi @18:14:07.57 SS.reconfigure :inhaling 1.13ml/s @18:14:07.62 SS.seek 25ml @18:14:32.48 Stroke completed -- waiting for pressure to equalize ... @18:14:32.50 SS.reconfigure :exhale @18:14:32.54 SS.hold @18:14:47.00 Intake.select :close @18:14:47.16 SSV.dial :exhaust @18:14:47.92 Exhaust.select :open @18:14:48.04 SS.seek 0ml @18:14:56.81 Exhaust.select :close @18:14:56.93 SSV.dial CBV @18:14:57.41 CBV.dial SSV @18:14:58.20 Allowing 130s for 25.0ml of remaining 800.0ml @18:14:58.22 Intake.select :open @18:15:00.39 Intake @15.6psia, vacuum from 10.0 to 13.0psi @18:15:00.41 SS.reconfigure :inhaling 1.13ml/s @18:15:00.46 SS.seek 25ml @18:15:24.73 Vacuum too high -- waiting up to 40 seconds @18:15:24.74 SS.reconfigure :exhale @18:15:24.80 SS.hold @18:15:33.51 Intake @15.1psia, vacuum from 9.6 to 12.6psi @18:15:33.53 SS.reconfigure :inhaling 0.91ml/s @18:15:33.58 SS.seek 25ml @18:15:34.70 Stroke completed -- waiting for pressure to equalize ... @18:15:34.71 SS.reconfigure :exhale @18:15:34.77 SS.hold @18:15:36.79 recoverPSRreagent08 @18:15:36.81 PTV.dial :puck @18:15:36.97 PBV.dial :waste1 @18:15:37.35 delay 30 seconds @18:15:41.74 Intake.select :close @18:15:41.90 SSV.dial :exhaust @18:15:42.67 Exhaust.select :open @18:15:42.79 SS.seek 0ml @18:15:51.61 Exhaust.select :close @18:15:51.72 SSV.dial CBV @18:15:51.74 CBV.dial SSV @18:15:52.53 Allowing 130s for 25.0ml of remaining 775.0ml @18:15:52.54 Intake.select :open @18:15:54.71 Intake @15.4psia, vacuum from 9.9 to 12.9psi @18:15:54.73 SS.reconfigure :inhaling 0.91ml/s @18:15:54.78 SS.seek 25ml @18:16:07.37 PBV.dial between PSV and waste1 @18:16:07.75 PSR.select :air @18:16:07.77 PSV.dial PSR @18:16:08.54 PS.reconfigure :fast @18:16:08.59 PS.seek 2.5ml @18:16:24.57 Stroke completed -- waiting for pressure to equalize ... @18:16:24.59 SS.reconfigure :exhale @18:16:24.64 SS.hold @18:16:32.76 PSV.dial PTV @18:16:33.54 delay 5 seconds @18:16:38.55 PSV.dial PSR @18:16:39.14 Intake.select :close @18:16:39.32 delay 5 seconds @18:16:39.35 SSV.dial :exhaust @18:16:40.12 Exhaust.select :open @18:16:40.24 SS.seek 0ml @18:16:44.35 PSV.dial PTV @18:16:45.11 delay 5 seconds @18:16:49.29 Exhaust.select :close @18:16:49.41 SSV.dial CBV @18:16:49.44 CBV.dial SSV @18:16:50.13 PSV.dial :air @18:16:50.23 Allowing 130s for 25.0ml of remaining 750.0ml @18:16:50.25 Intake.select :open @18:16:50.90 PS.seek 0ml @18:16:52.42 Intake @15.5psia, vacuum from 10.0 to 13.0psi @18:16:52.44 SS.reconfigure :inhaling 0.91ml/s @18:16:52.48 SS.seek 25ml @18:17:14.39 washCycleDA #2 of 2 @18:17:14.40 PSR.select :air @18:17:14.42 PSV.dial PSR @18:17:15.75 Pulling 0.5ml of PSR.wash @18:17:15.76 PSR.select :wash @18:17:15.88 PS.reconfigure :default @18:17:15.93 PS.seek 0.5ml @18:17:21.80 delay 5 seconds @18:17:21.85 Stroke completed -- waiting for pressure to equalize ... @18:17:22.25 SS.reconfigure :exhale @18:17:22.37 SS.hold @18:17:26.81 PSR.select :air @18:17:26.94 PS.seek 2ml @18:17:40.75 Intake.select :close @18:17:40.90 SSV.dial :exhaust @18:17:41.67 Exhaust.select :open @18:17:41.78 SS.seek 0ml @18:17:43.05 PTV.dial :puck @18:17:43.13 PSV.dial PTV @18:17:44.08 PBV.dial :waste1 @18:17:44.52 PS.seek 1.5ml @18:17:50.46 delay 3 seconds @18:17:50.60 Exhaust.select :close @18:17:50.72 SSV.dial CBV @18:17:50.74 CBV.dial SSV @18:17:51.53 Allowing 130s for 25.0ml of remaining 725.0ml @18:17:51.54 Intake.select :open @18:17:53.47 PBV.dial between PSV and waste1 @18:17:53.71 Intake @15.4psia, vacuum from 9.9 to 12.9psi @18:17:53.73 SS.reconfigure :inhaling 0.91ml/s @18:17:53.78 SS.seek 25ml @18:17:53.87 PS.seek 0ml @18:18:10.02 delay 2:00 @18:18:18.66 Vacuum too high -- waiting up to 40 seconds @18:18:18.68 SS.reconfigure :exhale @18:18:18.73 SS.hold @18:18:28.66 Intake @15.1psia, vacuum from 9.6 to 12.6psi @18:18:28.67 SS.reconfigure :inhaling 0.73ml/s @18:18:28.72 SS.seek 25ml @18:18:35.84 Stroke completed -- waiting for pressure to equalize ... @18:18:36.27 SS.reconfigure :exhale @18:18:36.36 SS.hold @18:18:47.52 Intake.select :close @18:18:47.68 SSV.dial :exhaust @18:18:48.45 Exhaust.select :open @18:18:48.57 SS.seek 0ml @18:18:57.35 Exhaust.select :close @18:18:57.47 SSV.dial CBV @18:18:57.50 CBV.dial SSV @18:18:58.29 Allowing 130s for 25.0ml of remaining 700.0ml @18:18:58.31 Intake.select :open @18:19:00.47 Intake @15.3psia, vacuum from 9.8 to 12.8psi @18:19:00.49 SS.reconfigure :inhaling 0.73ml/s @18:19:00.54 SS.seek 25ml @18:19:36.74 Stroke completed -- waiting for pressure to equalize ... @18:19:36.76 SS.reconfigure :exhale @18:19:36.80 SS.hold @18:19:59.57 Intake.select :close @18:19:59.73 SSV.dial :exhaust @18:20:00.49 Exhaust.select :open @18:20:00.61 SS.seek 0ml @18:20:09.40 Exhaust.select :close @18:20:09.52 SSV.dial CBV @18:20:09.55 CBV.dial SSV @18:20:10.13 recoverPSRreagent08 @18:20:10.15 PTV.dial :puck @18:20:10.22 PBV.dial :waste1 @18:20:10.58 delay 30 seconds @18:20:10.79 Allowing 130s for 25.0ml of remaining 675.0ml @18:20:10.81 Intake.select :open @18:20:12.98 Intake @15.4psia, vacuum from 9.9 to 12.9psi @18:20:13.00 SS.reconfigure :inhaling 0.73ml/s @18:20:13.05 SS.seek 25ml @18:20:40.60 PBV.dial between PSV and waste1 @18:20:40.97 PSR.select :air @18:20:40.99 PSV.dial PSR @18:20:41.76 PS.reconfigure :fast @18:20:41.87 PS.seek 2.5ml @18:20:42.35 Vacuum too high -- waiting up to 40 seconds @18:20:42.36 SS.reconfigure :exhale @18:20:42.42 SS.hold @18:20:55.25 Intake @15.5psia, vacuum from 10.0 to 13.0psi @18:20:55.27 SS.reconfigure :inhaling 0.59ml/s @18:20:55.79 SS.seek 25ml @18:21:04.71 Stroke completed -- waiting for pressure to equalize ... @18:21:04.72 SS.reconfigure :exhale @18:21:04.77 SS.hold @18:21:06.23 PSV.dial PTV @18:21:07.00 delay 5 seconds @18:21:12.01 PSV.dial PSR @18:21:12.76 delay 5 seconds @18:21:17.83 PSV.dial PTV @18:21:17.89 Intake.select :close @18:21:18.06 SSV.dial :exhaust @18:21:18.60 delay 5 seconds @18:21:18.84 Exhaust.select :open @18:21:18.95 SS.seek 0ml @18:21:23.61 PSV.dial :air @18:21:24.38 PS.seek 0ml @18:21:27.77 Exhaust.select :close @18:21:28.29 SSV.dial CBV @18:21:28.32 CBV.dial SSV @18:21:29.10 Allowing 130s for 25.0ml of remaining 650.0ml @18:21:29.12 Intake.select :open @18:21:31.29 Intake @15.3psia, vacuum from 9.8 to 12.8psi @18:21:31.31 SS.reconfigure :inhaling 0.59ml/s @18:21:31.35 SS.seek 25ml @18:21:48.42 getPMLReagent08 @18:21:48.43 PRV.dial :air @18:21:48.89 delay 5 seconds @18:21:53.91 PTV.dial :PRVmixing @18:21:55.25 delay 5 seconds @18:22:00.27 PRV.dial :ab2 @18:22:00.73 PTV.dial :PRVmixing @18:22:00.81 PSV.dial :mixloop @18:22:01.28 PS.reconfigure :default @18:22:01.33 PS.seek 0.3ml @18:22:05.26 delay 2 seconds @18:22:07.29 PRV.dial :air @18:22:07.81 PS.seek 0.55ml @18:22:11.14 addPMLReagent08 @18:22:11.15 PSV.dial :air @18:22:11.63 PS.seek 2ml @18:22:16.07 Stroke completed -- waiting for pressure to equalize ... @18:22:16.08 SS.reconfigure :exhale @18:22:16.13 SS.hold @18:22:27.29 PSV.dial :PML @18:22:27.76 PTV.dial :mixing @18:22:28.53 PBV.dial :waste1 @18:22:28.95 PS.seek 1.85ml @18:22:31.29 PBV.dial between PSV and waste1 @18:22:31.66 PS.seek 0ml @18:22:44.64 Intake.select :close @18:22:44.80 SSV.dial :exhaust @18:22:45.57 Exhaust.select :open @18:22:45.68 SS.seek 0ml @18:22:51.32 delay 2 seconds @18:22:53.34 delay 8:00 @18:22:54.68 Exhaust.select :close @18:22:54.79 SSV.dial CBV @18:22:54.81 CBV.dial SSV @18:22:55.60 Allowing 130s for 25.0ml of remaining 625.0ml @18:22:55.61 Intake.select :open @18:22:57.78 Intake @15.2psia, vacuum from 9.7 to 12.7psi @18:22:57.80 SS.reconfigure :inhaling 0.59ml/s @18:22:57.85 SS.seek 25ml @18:23:31.31 Vacuum too high -- waiting up to 40 seconds @18:23:31.33 SS.reconfigure :exhale @18:23:31.38 SS.hold @18:23:46.25 Intake @15.8psia, vacuum from 10.0 to 13.0psi @18:23:46.27 SS.reconfigure :inhaling 0.47ml/s @18:23:46.32 SS.seek 25ml @18:24:00.49 Stroke completed -- waiting for pressure to equalize ... @18:24:00.50 SS.reconfigure :exhale @18:24:00.55 SS.hold @18:24:21.05 Intake.select :close @18:24:21.21 SSV.dial :exhaust @18:24:21.98 Exhaust.select :open @18:24:22.10 SS.seek 0ml @18:24:30.88 Exhaust.select :close @18:24:30.99 SSV.dial CBV @18:24:31.01 CBV.dial SSV @18:24:31.80 Allowing 130s for 25.0ml of remaining 600.0ml @18:24:31.81 Intake.select :open @18:24:33.98 Intake @15.1psia, vacuum from 9.6 to 12.6psi @18:24:34.00 SS.reconfigure :inhaling 0.47ml/s @18:24:34.05 SS.seek 25ml @18:25:22.68 Vacuum too high -- waiting up to 40 seconds @18:25:22.70 SS.reconfigure :exhale @18:25:22.75 SS.hold @18:25:39.67 Intake @15.6psia, vacuum from 10.0 to 13.0psi @18:25:39.69 SS.reconfigure :inhaling 0.38ml/s @18:25:39.74 SS.seek 25ml @18:25:48.89 Stroke completed -- waiting for pressure to equalize ... @18:25:48.90 SS.reconfigure :exhale @18:25:48.95 SS.hold @18:26:07.34 Intake.select :close @18:26:07.50 SSV.dial :exhaust @18:26:08.27 Exhaust.select :open @18:26:08.38 SS.seek 0ml @18:26:17.19 Exhaust.select :close @18:26:17.30 SSV.dial CBV @18:26:17.33 CBV.dial SSV @18:26:18.11 Allowing 130s for 25.0ml of remaining 575.0ml @18:26:18.13 Intake.select :open @18:26:20.29 Intake @15.2psia, vacuum from 9.7 to 12.7psi @18:26:20.31 SS.reconfigure :inhaling 0.38ml/s @18:26:20.36 SS.seek 25ml @18:27:30.83 Stroke completed -- waiting for pressure to equalize ... @18:27:30.84 SS.reconfigure :exhale @18:27:30.89 SS.hold @18:28:03.76 Intake.select :close @18:28:03.92 SSV.dial :exhaust @18:28:04.69 Exhaust.select :open @18:28:04.81 SS.seek 0ml @18:28:13.67 Exhaust.select :close @18:28:13.78 SSV.dial CBV @18:28:13.82 CBV.dial SSV @18:28:14.58 Allowing 130s for 25.0ml of remaining 550.0ml @18:28:14.99 Intake.select :open @18:28:17.23 Intake @15.1psia, vacuum from 9.6 to 12.6psi @18:28:17.25 SS.reconfigure :inhaling 0.38ml/s @18:28:17.30 SS.seek 25ml @18:29:25.02 Vacuum too high -- waiting up to 40 seconds @18:29:25.04 SS.reconfigure :exhale @18:29:25.09 SS.hold @18:29:47.56 Intake @15.5psia, vacuum from 10.0 to 13.0psi @18:29:47.99 SS.reconfigure :inhaling 0.31ml/s @18:29:48.10 SS.seek 25ml @18:29:51.45 Stroke completed -- waiting for pressure to equalize ... @18:29:51.46 SS.reconfigure :exhale @18:29:51.51 SS.hold @18:30:09.89 Intake.select :close @18:30:10.05 SSV.dial :exhaust @18:30:10.81 Exhaust.select :open @18:30:10.93 SS.seek 0ml @18:30:19.70 Exhaust.select :close @18:30:19.81 SSV.dial CBV @18:30:20.32 CBV.dial SSV @18:30:21.05 Allowing 130s for 25.0ml of remaining 525.0ml @18:30:21.06 Intake.select :open @18:30:23.23 Intake @15.2psia, vacuum from 9.7 to 12.7psi @18:30:23.25 SS.reconfigure :inhaling 0.31ml/s @18:30:23.30 SS.seek 25ml @18:30:53.36 recoverPRVreagent08 @18:30:53.37 PTV.dial :mixing @18:30:53.56 PBV.dial :waste1 @18:30:53.93 delay 30 seconds @18:31:23.95 PBV.dial between PSV and waste1 @18:31:24.31 PSV.dial :air @18:31:24.79 PS.reconfigure :fast @18:31:24.84 PS.seek 2.5ml @18:31:48.90 PSV.dial :mixloop @18:31:49.38 delay 2 seconds @18:31:51.40 PSV.dial :air @18:31:51.99 delay 2 seconds @18:31:53.77 Stroke completed -- waiting for pressure to equalize ... @18:31:53.79 SS.reconfigure :exhale @18:31:53.84 SS.hold @18:31:54.00 PSV.dial :mixloop @18:31:54.48 delay 2 seconds @18:31:56.51 PSV.dial :air @18:31:57.13 PS.seek 0ml @18:32:20.59 washCycleDA #1 of 3 @18:32:20.60 PSR.select :air @18:32:20.62 PSV.dial PSR @18:32:21.96 Pulling 0.5ml of PSR.wash @18:32:21.97 PSR.select :wash @18:32:22.09 PS.reconfigure :default @18:32:22.14 PS.seek 0.5ml @18:32:28.15 delay 5 seconds @18:32:31.13 SS.reconfigure :default @18:32:31.18 SS.hold @18:32:31.25 Intake.select :close @18:32:31.41 ShallowSampler::Clogged in WCR -- Filtering too Slowly after 500.0ml @18:32:31.43 SSV.dial :exhaust @18:32:32.18 Exhaust.select :open @18:32:32.30 SS.reconfigure :exhale @18:32:32.35 SS.seek 0ml @18:32:33.17 PSR.select :air @18:32:33.29 PS.seek 2ml @18:32:41.52 Exhaust.select :close @18:32:41.64 Sampled 500.0ml @18:32:41.66 Drying puck before fixing for 5:00 @18:32:41.67 CBV.dial :waste2b @18:32:42.42 delay 1:00 @18:32:49.47 PTV.dial :puck @18:32:50.22 PSV.dial PTV @18:32:50.98 PBV.dial :waste1 @18:32:51.88 PS.seek 1.5ml @18:32:57.75 delay 3 seconds @18:33:00.77 PBV.dial between PSV and waste1 @18:33:01.13 PS.seek 0ml @18:33:17.31 delay 2:00 @18:33:42.45 CSV.dial :air @18:33:42.91 CS.reconfigure :fast @18:33:42.96 CS.seek 10ml @18:34:07.35 Pumping wcr puck from tube 2 up to 35psi... @18:34:07.37 CBV.dial SSV @18:34:08.63 CBV.dial between CSV and waste2b @18:34:09.59 CSV.dial between air and CTV @18:34:09.91 CS.seek 7.255ml @18:34:17.00 CTV.dial CSV,avoiding: :air @18:34:17.03 CSV.dial CTV @18:34:17.87 CS.reconfigure :pressurize @18:34:17.92 CS.seek 0ml @18:34:32.27 CBV.dial :waste2b @18:34:32.61 CS.reconfigure :default @18:34:32.67 CS.seek 0ml @18:34:38.28 delay 1:00 @18:35:17.34 recoverPSRreagent08 @18:35:17.35 PTV.dial :puck @18:35:17.37 PBV.dial :waste1 @18:35:17.72 delay 30 seconds @18:35:38.30 CTV.dial :air,avoiding: :intake @18:35:39.65 Fix #1 of 2 (with RNAlater) for 2:30 @18:35:39.66 CSV.dial CSR @18:35:40.09 Pulling 2ml of CSR.RNAlater @18:35:40.10 CSR.select :RNAlater @18:35:40.23 CS.seek 2ml @18:35:46.44 delay 5 seconds @18:35:47.73 PBV.dial between PSV and waste1 @18:35:48.09 PSR.select :air @18:35:48.11 PSV.dial PSR @18:35:48.88 PS.reconfigure :fast @18:35:48.92 PS.seek 2.5ml @18:35:51.46 CSR.select :air @18:35:51.58 CS.reconfigure :fast @18:35:51.63 CS.seek 10ml @18:36:11.34 Pumping wcr puck from tube 2 up to 35psi... @18:36:11.36 CBV.dial SSV @18:36:12.61 CBV.dial between CSV and waste2b @18:36:12.81 PSV.dial PTV @18:36:13.58 delay 5 seconds @18:36:14.08 CSV.dial between air and CTV @18:36:14.69 CS.seek 7.221ml @18:36:19.08 PSV.dial PSR @18:36:19.84 delay 5 seconds @18:36:21.82 CTV.dial CSV,avoiding: :air @18:36:21.85 CSV.dial CTV @18:36:23.24 CS.reconfigure :pressurize @18:36:23.29 CS.seek 0ml @18:36:24.86 PSV.dial PTV @18:36:25.61 delay 5 seconds @18:36:30.63 PSV.dial :air @18:36:31.39 PS.seek 0ml @18:36:38.77 delay 2:30 @18:36:54.90 washCycleDA #2 of 3 @18:36:54.91 PSR.select :air @18:36:54.93 PSV.dial PSR @18:36:56.26 Pulling 0.5ml of PSR.wash @18:36:56.27 PSR.select :wash @18:36:56.39 PS.reconfigure :default @18:36:56.44 PS.seek 0.5ml @18:37:02.34 delay 5 seconds @18:37:07.36 PSR.select :air @18:37:07.48 PS.seek 2ml @18:37:23.65 PTV.dial :puck @18:37:23.66 PSV.dial PTV @18:37:24.40 PBV.dial :waste1 @18:37:24.82 PS.seek 1.5ml @18:37:30.84 delay 3 seconds @18:37:33.86 PBV.dial between PSV and waste1 @18:37:34.22 PS.seek 0ml @18:37:50.37 delay 2:00 @18:39:08.80 CBV.dial :waste2b @18:39:09.14 CS.reconfigure :default @18:39:09.19 CS.seek 0ml @18:39:13.53 delay 1:00 @18:39:50.40 recoverPSRreagent08 @18:39:50.41 PTV.dial :puck @18:39:50.42 PBV.dial :waste1 @18:39:50.77 delay 30 seconds @18:40:13.55 CTV.dial :air,avoiding: :intake @18:40:14.88 Fix #2 of 2 (with RNAlater) for 2:30 @18:40:14.89 CSV.dial CSR @18:40:15.34 Pulling 2ml of CSR.RNAlater @18:40:15.35 CSR.select :RNAlater @18:40:15.47 CS.seek 2ml @18:40:20.79 PBV.dial between PSV and waste1 @18:40:21.15 PSR.select :air @18:40:21.17 PSV.dial PSR @18:40:21.43 delay 5 seconds @18:40:22.04 PS.reconfigure :fast @18:40:22.09 PS.seek 2.5ml @18:40:26.45 CSR.select :air @18:40:26.57 CS.reconfigure :fast @18:40:26.62 CS.seek 10ml @18:40:46.19 PSV.dial PTV @18:40:46.90 Pumping wcr puck from tube 2 up to 35psi... @18:40:46.93 CBV.dial SSV @18:40:46.99 delay 5 seconds @18:40:48.19 CBV.dial between CSV and waste2b @18:40:49.15 CSV.dial between air and CTV @18:40:49.75 CS.seek 7.221ml @18:40:52.01 PSV.dial PSR @18:40:52.77 delay 5 seconds @18:40:57.10 CTV.dial CSV,avoiding: :air @18:40:57.13 CSV.dial CTV @18:40:57.79 PSV.dial PTV @18:40:58.50 CS.reconfigure :pressurize @18:40:58.57 CS.seek 0ml @18:40:58.60 delay 5 seconds @18:41:03.63 PSV.dial :air @18:41:04.39 PS.seek 0ml @18:41:13.64 delay 2:30 @18:41:28.08 washCycleDA #3 of 3 @18:41:28.09 PSR.select :air @18:41:28.11 PSV.dial PSR @18:41:29.44 Pulling 0.5ml of PSR.wash @18:41:29.45 PSR.select :wash @18:41:29.57 PS.reconfigure :default @18:41:29.62 PS.seek 0.5ml @18:41:35.61 delay 5 seconds @18:41:40.63 PSR.select :air @18:41:40.76 PS.seek 2ml @18:41:56.95 PTV.dial :puck @18:41:56.97 PSV.dial PTV @18:41:57.72 PBV.dial :waste1 @18:41:58.16 PS.seek 1.5ml @18:42:04.21 delay 3 seconds @18:42:07.23 PBV.dial between PSV and waste1 @18:42:07.60 PS.seek 0ml @18:42:23.86 delay 2:00 @18:43:43.67 CBV.dial :waste2b @18:43:43.99 CS.reconfigure :default @18:43:44.05 CS.seek 0ml @18:43:48.90 delay 1:00 @18:44:23.89 recoverPSRreagent08 @18:44:24.52 PTV.dial :puck @18:44:24.54 PBV.dial :waste1 @18:44:24.91 delay 30 seconds @18:44:48.93 CTV.dial :air,avoiding: :intake @18:44:54.93 PBV.dial between PSV and waste1 @18:44:55.31 PSR.select :air @18:44:55.33 PSV.dial PSR @18:44:56.10 PS.reconfigure :fast @18:44:56.15 PS.seek 2.5ml @18:45:20.08 PSV.dial PTV @18:45:21.33 delay 5 seconds @18:45:26.35 PSV.dial PSR @18:45:27.09 delay 5 seconds @18:45:32.12 PSV.dial PTV @18:45:32.86 delay 5 seconds @18:45:37.88 PSV.dial :air @18:45:38.77 PS.seek 0ml @18:46:02.14 Gate.power :camera,:ON @18:46:02.15 Mixing DA Substrates @18:46:02.16 PSR.select :air @18:46:02.18 PSV.dial PSR @18:46:03.59 Pulling 0.25ml of PSR.sub1 @18:46:03.61 PSR.select :sub1 @18:46:03.82 PS.reconfigure :default @18:46:03.93 PS.seek 0.25ml @18:46:07.30 delay 5 seconds @18:46:12.33 Pulling 0.25ml of PSR.sub2 @18:46:12.34 PSR.select :sub2 @18:46:12.46 PS.seek 0.5ml @18:46:15.83 delay 5 seconds @18:46:20.85 PSR.select :air @18:46:20.96 PS.seek 2ml @18:46:37.17 PTV.dial :puck @18:46:37.18 PSV.dial PTV @18:46:37.97 PBV.dial :waste1 @18:46:38.40 PS.seek 1.5ml @18:46:44.33 delay 3 seconds @18:46:47.35 PBV.dial between PSV and waste1 @18:46:47.72 PS.seek 0ml @18:47:03.83 delay 10 seconds @18:47:13.86 recoverPSRreagent08 PTV.dial :puck @18:47:13.88 PBV.dial :waste1 @18:47:14.26 delay 30 seconds @18:47:44.29 PBV.dial between PSV and waste1 @18:47:44.67 PSR.select :air @18:47:44.69 PSV.dial PSR @18:47:45.46 PS.reconfigure :fast @18:47:45.51 PS.seek 2.5ml @18:48:09.92 PSV.dial PTV @18:48:10.68 delay 5 seconds @18:48:15.70 PSV.dial PSR @18:48:16.47 delay 5 seconds @18:48:21.49 PSV.dial PTV @18:48:22.25 delay 5 seconds @18:48:27.27 PSV.dial :air @18:48:28.03 PS.seek 0ml @18:48:52.14 Additional air clear @18:48:52.15 PSR.select :air @18:48:52.17 PSV.dial PSR @18:48:53.51 Pulling 2ml of PSR.air @18:48:53.52 PSR.select :air @18:48:53.55 PS.reconfigure :default @18:48:53.59 PS.seek 2ml @18:49:14.89 delay 5 seconds @18:49:19.91 PTV.dial :puck @18:49:19.92 PSV.dial PTV @18:49:20.68 PBV.dial :waste1 @18:49:21.12 PS.seek 1.5ml @18:49:27.10 delay 3 seconds @18:49:30.12 PBV.dial between PSV and waste1 @18:49:30.49 delay 1 second @18:49:31.51 PSV.dial :waste1 @18:49:32.59 PS.seek 0ml @18:49:48.97 Processing Puck Heater at 28C before imaging @18:49:48.98 PH.coast @18:49:49.09 Imaging da puck in Processing Clamp while storing it to tube 1 @18:49:49.77 SC.reconfigure :tight @18:49:49.82 SC.seek 1 @18:49:53.37 SC.configure :limp @18:49:53.60 Elbow.reconfigure :tight @18:49:53.65 Elbow.seek 1 @18:49:55.41 Forearm.reconfigure :tight @18:49:55.46 Forearm.seek 1 + 450 counts @18:49:56.52 SE.reconfigure :pick @18:49:56.58 SE.seek :beamBreak @18:49:58.30 SE.configure :default @18:49:58.47 Elbow.reconfigure :limp @18:49:58.52 Forearm.reconfigure :loose @18:49:58.56 Forearm.seek 1 @18:49:58.83 Elbow.hold @18:49:58.90 Elbow.reconfigure :tight @18:49:59.00 SE.seek 21.05 Pucks below Top Plate @18:49:59.44 Forearm.force -20 @18:49:59.48 Forearm.seek 1 + 311 counts @18:49:59.79 Forearm.reconfigure :tight @18:49:59.84 Forearm.seek 1 + 450 counts @18:50:00.06 SE.seek 22.05 Pucks below Top Plate @18:50:00.63 Forearm.reconfigure :loose @18:50:00.68 Forearm.seek :retract @18:50:01.66 Elbow.seek PC @18:50:04.16 Elbow.reconfigure :loose @18:50:04.26 Forearm.seek PC @18:50:05.21 Hand.close @18:50:05.84 PC.seek :open @18:50:10.62 Forearm.seek :retract @18:50:11.59 Elbow.reconfigure :tight @18:50:12.07 Elbow.seek 1 @18:50:14.72 Forearm.reconfigure :tight @18:50:14.77 Forearm.seek 1 + 450 counts @18:50:15.80 Hand.open @18:50:16.52 SE.seek 21.7 Pucks below Top Plate @18:50:16.78 SE.reconfigure :seal @18:50:16.83 SE.seek 20.74 Pucks below Top Plate @18:50:17.35 Lights, camera, ACTION! @18:50:17.38 PuckCamera: snap -bin=2,2 -auto /var/log/esp/da23oct0318h550ml.tif @18:50:36.89 Waiting >20 seconds for da to relinquish FlushPuck @18:50:40.43 SX HX9/16 (USB:06): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 19.739 seconds /var/log/esp/da23oct0318h550ml.tif: TIFF Upload Complete @18:50:40.46 PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da23oct0318h550ml15s.tif @18:50:56.11 SX HX9/16 (USB:06): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15 seconds /var/log/esp/midres/da23oct0318h550ml15s.tif: TIFF Upload Complete @18:50:56.14 PuckCamera: snap -bin=2,2 20.0 /var/log/esp/midres/da23oct0318h550ml20s.tif @18:51:16.75 SX HX9/16 (USB:06): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 20 seconds /var/log/esp/midres/da23oct0318h550ml20s.tif: TIFF Upload Complete @18:51:16.77 Gate.power :camera,:OFF @18:51:16.79 DAfans.email "19.739 sec auto-exposure",Subject: "da23oct0318h550ml images!" @18:51:17.24 Elbow.seek 1 @18:51:17.48 Forearm.seek 1 + 450 counts @18:51:17.56 SE.reconfigure :gently @18:51:17.63 SE.seek 21.45 Pucks below Top Plate @18:51:18.33 Elbow.reconfigure :limp @18:51:18.38 Forearm.reconfigure :loose @18:51:18.45 Forearm.seek 1 @18:51:18.71 Elbow.hold @18:51:18.78 Elbow.reconfigure :tight @18:51:18.83 SE.reconfigure :default @18:51:18.89 SE.seek 21.64 Pucks below Top Plate @18:51:19.38 Forearm.force -20 @18:51:19.45 Forearm.seek 1 + 304 counts @18:51:20.01 Forearm.reconfigure :tight @18:51:20.07 Forearm.seek 1 + 450 counts @18:51:20.42 SE.seek :down @18:51:21.80 SE.stop @18:51:21.85 Forearm.reconfigure :loose @18:51:23.18 Forearm.seek :retract @18:51:24.37 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @18:51:24.90 Elbow.seek FlushPuck::Garage @18:51:26.87 Elbow.reconfigure :loose @18:51:27.11 Forearm.seek :garage @18:51:28.12 Hand.close @18:51:28.98 Forearm.seek :retract @18:51:29.96 Elbow.reconfigure :tight @18:51:30.09 Elbow.seek PC @18:51:31.50 Elbow.reconfigure :loose @18:51:31.70 Forearm.reconfigure :tight @18:51:31.82 Forearm.seek PC @18:51:33.40 PC.seek :closed @18:51:38.27 Processing Clamp onSpring/offSpring for 16/26 samples, on/off torque 1.64 @18:51:38.41 Hand.open @18:51:39.25 Forearm.reconfigure :loose @18:51:39.34 Forearm.seek :retract @18:51:40.37 PSR.select :air @18:51:40.44 Storing wcr puck in Collection Clamp to tube 1 @18:51:40.49 PSV.dial PSR @18:51:41.24 Pulling 0.5ml of PSR.flush @18:51:41.32 PSR.select :flush @18:51:42.63 Elbow.reconfigure :tight @18:51:42.83 Elbow.seek 1 @18:51:42.92 PS.seek 0.5ml @18:51:45.78 Forearm.reconfigure :tight @18:51:45.90 Forearm.seek 1 + 450 counts @18:51:47.14 SE.reconfigure :pick @18:51:47.26 SE.seek :beamBreak @18:51:49.05 SE.configure :default @18:51:49.29 delay 5 seconds @18:51:49.46 Elbow.reconfigure :limp @18:51:49.56 Forearm.reconfigure :loose @18:51:49.65 Forearm.seek 1 @18:51:49.96 Elbow.hold @18:51:50.08 Elbow.reconfigure :tight @18:51:50.30 SE.seek 22.05 Pucks below Top Plate @18:51:51.02 Forearm.force -20 @18:51:51.12 Forearm.seek 1 + 312 counts @18:51:51.59 Forearm.reconfigure :tight @18:51:51.65 Forearm.seek 1 + 450 counts @18:51:51.85 SE.seek 23.05 Pucks below Top Plate @18:51:52.03 upsync @18:51:52.44 Forearm.reconfigure :loose @18:51:52.49 Forearm.seek :retract @18:51:53.43 Elbow.seek CC @18:51:54.34 PSR.select :air @18:51:54.45 PS.seek 2.5ml @18:51:55.06 Elbow.reconfigure :loose @18:51:55.17 Forearm.seek CC @18:51:56.13 Hand.close @18:51:56.71 CC.seek :open @18:52:01.96 Forearm.seek :retract @18:52:02.91 Elbow.reconfigure :tight @18:52:02.97 Elbow.seek 1 @18:52:04.77 Elbow.reconfigure :limp @18:52:04.82 Forearm.seek 1 @18:52:05.79 Elbow.hold @18:52:05.86 Elbow.reconfigure :tight @18:52:05.91 Hand.open @18:52:06.71 Forearm.force -20 @18:52:06.76 Forearm.seek 1 + 321 counts @18:52:07.14 Forearm.reconfigure :tight @18:52:07.20 Forearm.seek 1 + 450 counts @18:52:07.44 SE.seek :down @18:52:08.58 Forearm.reconfigure :loose @18:52:08.65 Forearm.seek :retract @18:52:08.68 SE.stop @18:52:09.65
Starting finalPurge @18:52:15.76 delay 1 second @18:52:16.78 PRV.dial :waste2,avoiding: :ab2 @18:52:18.63 PTV.dial :PRVmixing @18:52:19.94 PSV.dial :mixloop @18:52:20.99 PS.seek :empty @18:52:28.81
Waiting >20 seconds for psFlush to relinquish FlushPuck @18:52:47.50 PSR.select :air @18:52:47.52 PSV.dial PSR @18:52:48.57 Pulling 0.5ml of PSR.flush @18:52:48.58 PSR.select :flush @18:52:48.70 PS.seek 0.5ml @18:52:54.57 delay 5 seconds @18:52:59.59 PSR.select :air @18:52:59.71 PS.seek 2.5ml @18:53:20.91 delay 1 second @18:53:21.93 PBV.dial :waste1 @18:53:22.29 PTV.dial :mixing @18:53:23.11 PSV.dial :mixloop @18:53:24.29 PS.seek :empty @18:53:50.66 PSV.dial PSR @18:53:51.69 PSR.select :flush @18:53:51.81 PS.seek 1ml @18:54:02.85 PSR.select :air @18:54:02.97 PS.seek 2.5ml @18:54:19.28 PSV.dial PTV @18:54:20.04 PTV.dial :puck @18:54:20.77 PBV.dial :waste1 @18:54:20.85 PS.seek 0ml @18:54:47.19 PSV.dial PSR @18:54:47.94 PSR.select :flush @18:54:48.06 PS.seek 0.5ml @18:54:54.09 PSR.select :air @18:54:54.21 PS.seek 2.5ml @18:55:15.50 PSV.dial PTV @18:55:16.25 PBV.dial PSV @18:55:16.82 PS.seek 0ml @18:55:43.16 PSV.dial PBV @18:55:43.74 PS.seek 2.5ml @18:56:10.06 PSV.dial :waste1 @18:56:10.82 PS.seek 0ml @18:56:37.19 PSV.dial PSR @18:56:37.66 PSR.select :air @18:56:37.68 PS.seek 2.5ml @18:57:04.12 PSV.dial PTV @18:57:04.86 PBV.dial PSV @18:57:04.94 PS.seek 0ml @18:57:31.28 PSV.dial PBV @18:57:31.76 PS.seek 2.5ml @18:57:58.09 PSV.dial :waste1 @18:57:58.83 PS.seek 0ml @18:58:25.12 PSV.dial PSR @18:58:25.59 PSR.select :flush @18:58:25.71 PS.seek 1ml @18:58:36.81 PSR.select :air @18:58:36.93 PS.seek 2.5ml @18:58:53.09 PSV.dial PTV @18:58:53.83 PTV.dial :puck @18:58:53.84 PBV.dial :waste1 @18:58:54.39 PS.seek 0ml @18:59:20.71 PSV.dial PSR @18:59:21.47 PSR.select :flush @18:59:22.04 PS.seek 0.5ml @18:59:28.09 PSR.select :air @18:59:28.21 PS.seek 2.5ml @18:59:49.50 PSV.dial PTV @18:59:50.25 PBV.dial PSV @18:59:50.93 PS.seek 0ml @19:00:17.31 PSV.dial PBV @19:00:17.89 PS.seek 2.5ml @19:00:44.28 PSV.dial :waste1 @19:00:45.05 PS.seek 0ml @19:01:11.37 PSV.dial PSR @19:01:11.84 PSR.select :air @19:01:11.86 PS.seek 2.5ml @19:01:38.18 PSV.dial PTV @19:01:38.92 PBV.dial PSV @19:01:39.00 PS.seek 0ml @19:02:05.37 PSV.dial PBV @19:02:05.84 PS.seek 2.5ml @19:02:32.21 PSV.dial :waste1 @19:02:32.95 PS.seek 0ml @19:02:59.50
Loading FlushPuck into Collection Clamp from Processing Clamp @19:02:59.69 Elbow.seek PC @19:03:02.17 Elbow.reconfigure :loose @19:03:02.28 Forearm.seek PC @19:03:03.26 Hand.close @19:03:03.83 PC.seek :open @19:03:08.55 Forearm.seek :retract @19:03:09.50 Elbow.reconfigure :tight @19:03:09.54 Elbow.seek CC @19:03:13.18 Elbow.reconfigure :loose @19:03:13.28 Forearm.reconfigure :tight @19:03:13.32 Forearm.seek CC @19:03:14.53 CC.seek :closed @19:03:19.07 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.31 @19:03:19.14 Hand.open @19:03:19.77 Forearm.reconfigure :loose @19:03:19.82 Forearm.seek :retract @19:03:20.78 Flushing the Collection Valve::Manifold @19:03:20.90 CSV.dial CSR @19:03:21.45 Pulling 5ml of CSR.flush @19:03:21.46 CSR.select :flush @19:03:21.58 CS.seek 5ml @19:03:35.37 delay 5 seconds @19:03:40.39 CSR.select :air @19:03:40.56 CS.reconfigure :fast @19:03:40.61 CS.seek 7.5ml @19:03:47.34 CSV.dial :waste @19:03:48.61 CS.reconfigure :default @19:03:48.66 CS.seek 0ml @19:04:08.84 Flushing lower manifold's waste1 port @19:04:08.97 CSV.dial :flush @19:04:09.43 CS.reconfigure :fast @19:04:09.47 CS.seek 5ml @19:04:22.41 delay 5 seconds @19:04:27.43 CSV.dial :air @19:04:27.94 CS.seek 7ml @19:04:33.44 CSV.dial CTV @19:04:33.47
CTV.dial CSV @19:04:34.83 CBV.dial :waste1 @19:04:35.58 CS.seek 0ml @19:04:52.31 Flushing lower manifold's waste2 port @19:04:52.44 CSV.dial :flush @19:04:53.27 CS.seek 5ml @19:05:06.12 delay 5 seconds @19:05:11.14 CSV.dial :air @19:05:11.64 CS.seek 7ml @19:05:17.44 CSV.dial CTV @19:05:17.47
CTV.dial CSV @19:05:17.97 CBV.dial :waste2 @19:05:19.00 CS.seek 0ml @19:05:35.50 Flushing lower manifold's waste2b port @19:05:36.01 CSV.dial :flush @19:05:36.82 CS.seek 5ml @19:05:49.40 delay 5 seconds @19:05:54.42 CSV.dial :air @19:05:54.95 CS.seek 7.001ml @19:06:00.41 CSV.dial CTV @19:06:00.43
CTV.dial CSV @19:06:00.89 CBV.dial :waste2b @19:06:01.93 CS.seek 0ml @19:06:18.47 Disinfecting external Intake with kill @19:06:18.59 CSV.dial CSR @19:06:19.03 Pulling 8ml of CSR.kill @19:06:19.04 CSR.select :kill @19:06:19.16 CS.seek 8ml @19:06:38.90 delay 5 seconds @19:06:43.92 CSR.select :air @19:06:44.04 CS.seek 10ml @19:06:49.56 CSV.dial CBV @19:06:49.59
CBV.dial CSV @19:06:50.64 CTV.dial :intake @19:06:51.40 Intake.select :open @19:06:51.51 CS.seek 2ml @19:07:10.31 Intake.select :close @19:07:10.42 CTV.dial :air @19:07:11.19 CS.reconfigure :default @19:07:11.24 CS.seek 0ml @19:07:17.18 CSV.dial CSR @19:07:18.31 Pulling 5ml of CSR.flush @19:07:18.32 CSR.select :flush @19:07:18.44 CS.seek 5ml @19:07:32.00 delay 5 seconds @19:07:37.01 CSR.select :air @19:07:37.13 CS.seek 10ml @19:07:50.68 CSV.dial CBV @19:07:51.67 CS.seek 0ml @19:08:18.09 Garaging FlushPuck in Collection Clamp @19:08:18.16 Elbow.reconfigure :tight @19:08:18.21 Elbow.seek CC @19:08:18.50 Elbow.reconfigure :loose @19:08:18.61 Forearm.seek CC @19:08:19.65 Hand.close @19:08:20.32 CC.seek :open @19:08:24.96 Forearm.seek :retract @19:08:25.95 Elbow.reconfigure :tight @19:08:25.99 Elbow.seek :garage @19:08:28.89 Elbow.reconfigure :loose @19:08:29.44 Forearm.reconfigure :tight @19:08:29.49 Forearm.seek :garage @19:08:30.46 Hand.open @19:08:31.13 Forearm.reconfigure :loose @19:08:31.18 Forearm.seek :retract @19:08:32.13 Completed [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] begun 16:51:42 @19:08:32.19 Asleep after completing DA_WCR_100323 assays @19:08:32.20 GoodNews.email "{1 => 24,\n 2 => 9,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@19:00:52, 25% flow, 20.8C, 14% humidity, 14.7psia, 13.769V, 0.302A, 91.409Ah, 4.16W\nSampled 500.0ml",Subject: "Asleep after completing DA_WCR_100323 assays" @19:08:36.20 upsync + ESPwhich DA_WCR_100923.rb + fn=DA_WCR_100923.rb + IFS=: + set /home/esp/esp2/mission . + fullPath=/home/esp/esp2/mission/DA_WCR_100923.rb + [ -f /home/esp/esp2/mission/DA_WCR_100923.rb ] + realpath /home/esp/esp2/mission/DA_WCR_100923.rb + return 0 + scriptPath=/home/esp/esp2local/mission/DA_WCR_100923.rb + exec ruby /home/esp/esp2/lib/esp.rb /home/esp/esp2local/mission/DA_WCR_100923.rb @14:30:22.67UTC09-Oct-23
ESP2 v3.42 I2C Gateway v3.20 for core bus @14:30:25.31 Configuration for short puck jake 1/11/21 by brent@mbari.org /home/esp/esp2/type/shallow/jake/configure.rb @14:30:28.96 Polling Can every 10:00 @14:30:29.02 Loading /home/esp/esp2local/mission/DA_WCR_100923.rb mission @14:30:29.08 Preparing to sleep until + 00:00:05 @14:30:29.09 GoodNews.email "Starting DA_WCR_100923 w/pucks in tube 2\n{}\nCTD data unavailable\nCan@14:30:28, 28% flow, 17.2C, 16% humidity, 14.6psia, 13.764V, 0.218A, 119.995Ah, 3.00W",Subject: "DA_WCR_100923 begun" @14:30:29.14
Asleep til + 00:00:05 @14:30:29.17 Continuing at 14:30 @14:30:29.42 Gate.power :core,:OFF @14:30:29.43 Gate.power :raw,:OFF @14:30:29.44 Shutdown @14:30:34.05
Powering core bus @14:30:39.73 Gate.power :raw,:ON @14:30:40.72 CS.configure :default @14:30:40.77 PS.configure :default @14:30:40.82 SS.configure :default @14:30:41.20 CC.configure :default @14:30:41.29 PC.configure :default @14:30:41.35 Forearm.configure :loose @14:30:41.40 Elbow.configure :loose @14:30:41.47 SC.configure :loose @14:30:41.52 SE.configure :default @14:30:41.57 CH.configure :default @14:30:41.61 PH.configure :default @14:30:41.64 All dwarf controllers running firmware version 3.85 @14:30:41.75 Valve.reset @14:30:41.98 Intake.select :close @14:30:42.03 Exhaust.select :close @14:30:42.07 CTV.dial :air @14:30:42.16 PSV.dial :air @14:30:42.21 CSV.dial :waste1 @14:30:42.40
Forearm.home.seek :retract @14:30:42.75 SSV.dial :spit,avoiding: :exhaust @14:30:43.14 Hand.close @14:30:43.18
Elbow.reconfigure :loose @14:30:43.27 Elbow.jog 1500 @14:30:43.45 PS.home.seek 0ml @14:30:43.62 CS.reconfigure :fast @14:30:43.66 SS.home.seek 0ml @14:30:43.74 CS.home.seek 0ml @14:30:43.82
Elbow.reconfigure :loose @14:30:43.90 Elbow.home.seek :clear @14:30:48.41 SE.home.seek :down @14:30:49.09 SC.reconfigure :tight @14:30:49.13 SC.home.seek :initial @14:30:51.68 PS.stop @14:30:59.75 GoodNews.email "Then, in tube 2: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_100923.rb:14\n{1 => 24,\n 2 => 9,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@14:30:29, 28% flow, 17.2C, 16% humidity, 14.6psia, 13.764V, 0.212A, 119.995Ah, 2.92W",Subject: "Asleep until 14:30" @14:31:01.49 upsync @14:31:15.18 upsync @14:31:17.49
SC.configure :limp @14:31:17.55 Forearm.seek :retract @14:31:17.62 Elbow.seek :hammer @14:31:18.66 Forearm.reconfigure :tight @14:31:18.71 Forearm.seek retracted @14:31:18.99 CC.home.seek :open @14:31:19.01 PC.home.seek :open @14:31:27.05
Phase [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] @14:31:27.06 Notice.email "At 14:31:26 began in tube 2: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_100923.rb:14\n{1 => 24,\n 2 => 9,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@14:31:27, 28% flow, 17.3C, 16% humidity, 14.6psia, 13.720V, 0.278A, 120.001Ah, 3.82W",Subject: "Started DA_WCR_100923 phase initialPurge; da [1000, 1000] #da and wsr are run simultanelously" @14:31:27.09
Starting initialPurge @14:31:27.11 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:31:27.17 Hand.open @14:31:27.89 Elbow.reconfigure :tight @14:31:27.94 Elbow.seek FlushPuck::Garage @14:31:29.83 Elbow.reconfigure :loose @14:31:29.93 Forearm.reconfigure :loose @14:31:29.98 Forearm.seek :garage @14:31:30.92 Hand.close @14:31:31.62 Forearm.seek :retract @14:31:32.62 Elbow.reconfigure :tight @14:31:32.67 Elbow.seek CC @14:31:35.63 Elbow.reconfigure :loose @14:31:35.73 Forearm.reconfigure :tight @14:31:35.78 Forearm.seek CC @14:31:37.05 CC.seek :closed @14:31:41.68 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.34 @14:31:41.74 Hand.open @14:31:42.01 upsync @14:31:42.43
Forearm.reconfigure :loose @14:31:42.48 Forearm.seek :retract @14:31:43.41 Flushing the Collection Valve::Manifold @14:31:43.54 CSV.dial CSR @14:31:44.82 Pulling 5ml of CSR.flush @14:31:44.83 CSR.select :flush @14:31:44.96 CS.reconfigure :default @14:31:45.43 CS.seek 5ml @14:31:59.26 delay 5 seconds @14:32:04.28 CSR.select :air @14:32:04.46 CS.reconfigure :fast @14:32:04.50 CS.seek 7.5ml @14:32:11.23 CSV.dial :waste @14:32:12.51 CS.reconfigure :default @14:32:12.56 CS.seek 0ml @14:32:32.60 Purging external Intake @14:32:32.73 CSV.dial :flush @14:32:33.18 CS.reconfigure :fast @14:32:33.23 CS.seek 10ml @14:32:57.60 delay 5 seconds @14:33:02.62 CSV.dial CBV @14:33:02.66
CBV.dial CSV @14:33:03.40 CTV.dial :intake @14:33:04.21 Intake.select :open @14:33:04.32 CS.seek 0ml @14:33:27.85 Intake.select :close @14:33:27.95 CTV.dial :air @14:33:28.74 Flushing upper manifold @14:33:28.86 CSV.dial :flush @14:33:29.61 CS.seek 5ml @14:33:42.26 delay 5 seconds @14:33:47.27 CSV.dial :air @14:33:47.77 CS.seek 7ml @14:33:53.38 CSV.dial CBV @14:33:53.41
CBV.dial CSV @14:33:54.46 CTV.dial :air @14:33:54.54 CS.seek 0ml @14:34:11.23 delay 7 seconds @14:34:18.25 CS.seek 3ml @14:34:26.32 CSV.dial :waste @14:34:26.78 CS.seek 0ml @14:34:34.52 CSV.dial :flush @14:34:34.96 CS.seek 3ml @14:34:43.13 delay 5 seconds @14:34:48.15 CSV.dial :air @14:34:48.77 CS.seek 5ml @14:34:54.32 CSV.dial :air @14:34:54.40 CS.seek 0ml @14:35:06.54 delay 7 seconds @14:35:13.56 CS.seek 3ml @14:35:21.60 CSV.dial :waste @14:35:22.32 CS.seek 0ml @14:35:29.91 Garaging FlushPuck in Collection Clamp @14:35:29.98 Elbow.reconfigure :tight @14:35:30.03 Elbow.seek CC @14:35:30.33 Elbow.reconfigure :loose @14:35:30.44 Forearm.seek CC @14:35:31.49 Hand.close @14:35:32.12 CC.seek :open @14:35:37.18 Forearm.seek :retract @14:35:38.15 Elbow.reconfigure :tight @14:35:38.20 Elbow.seek :garage @14:35:41.11 Elbow.reconfigure :loose @14:35:41.21 Forearm.reconfigure :tight @14:35:41.26 Forearm.seek :garage @14:35:42.32 Hand.open @14:35:43.05 Forearm.reconfigure :loose @14:35:43.10 Forearm.seek :retract @14:35:44.09 da sampling at most 1000ml, wcr at most 1000ml @14:35:44.12 Loading sh1 puck from tube 2 into Collection Clamp @14:35:44.14 DAfans.email "Started da in tube 2 at 14:35:44UTC09-Oct-23\n{1 => 24,\n 2 => 9,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@14:31:27, 28% flow, 17.3C, 16% humidity, 14.6psia, 13.720V, 0.278A, 120.001Ah, 3.82W",Subject: "da sampling at most 1000ml, wcr at most 1000ml" @14:35:44.42
SC.reconfigure :tight @14:35:44.47 SC.seek 2 @14:35:51.25 SC.configure :limp @14:35:51.47 Elbow.reconfigure :tight @14:35:51.52 Elbow.seek 2 @14:35:53.52 Forearm.reconfigure :tight @14:35:53.57 Forearm.seek 2 + 450 counts @14:35:54.63 SE.reconfigure :pick @14:35:54.69 SE.seek :beamBreak @14:35:57.30 SE.configure :default @14:35:57.68 Elbow.seek 2 @14:35:57.92 Forearm.seek 2 + 300 counts @14:35:58.23 Forearm.reconfigure :loose @14:35:58.28 Hand.close @14:35:58.88 SE.seek :down @14:35:58.92 upsync @14:35:59.11
Forearm.seek :retract @14:36:00.49 Elbow.seek CC @14:36:01.16 SE.stop @14:36:02.13
Elbow.reconfigure :loose @14:36:02.24 Forearm.reconfigure :tight @14:36:02.29 Forearm.seek CC @14:36:03.50 CC.seek :closed @14:36:08.09 Collection Clamp onSpring/offSpring for 14/27 samples, on/off torque 2.21 @14:36:08.10 Hand.open @14:36:08.80 Forearm.reconfigure :loose @14:36:08.85 Forearm.seek :retract @14:36:09.82 collectSample 1000ml w/bubble point of 24psi @14:36:09.83 Priming 7ml @14:36:09.84 Intake.select :close @14:36:09.86 Exhaust.select :close @14:36:09.87 SS.hold @14:36:10.08 Calibrating pressure and temperature sensors @14:36:10.11 CTV.dial :air @14:36:10.19 SSV.dial :spit,avoiding: :exhaust @14:36:10.38 SS.reconfigure :fast @14:36:10.43 SS.seek 5ml @14:36:13.44 SSV.dial CBV @14:36:13.48
CBV.dial SSV @14:36:14.78 delay 3.8 seconds @14:36:18.72 Sensor limits: Intake max 113.9psia; Outlet max 106.1psia @14:36:18.73 SSV.dial :spit @14:36:20.04 SS.seek 0ml @14:36:22.59 SSV.dial CBV @14:36:23.91 CTV.dial :intake @14:36:24.71 SSV.dial CBV @14:36:24.74
CBV.dial SSV @14:36:24.92 Allowing 40s for 7.0ml of remaining 7.0ml @14:36:24.93 Intake.select :open @14:36:27.09 Intake @14.5psia, vacuum from 9.0 to 12.0psi @14:36:27.11 SS.reconfigure :inhaling 2.20ml/s @14:36:27.16 SS.seek 7ml @14:36:33.57 Stroke completed -- waiting for pressure to equalize ... @14:36:33.58 SS.reconfigure :exhale @14:36:33.63 SS.hold @14:36:40.55 Intake.select :close @14:36:40.70 SSV.dial :exhaust @14:36:41.49 Exhaust.select :open @14:36:41.60 SS.seek 0ml @14:36:45.43 Exhaust.select :close @14:36:45.55 Sampling 1000.0ml @14:36:45.56 Intake.select :close @14:36:45.57 Exhaust.select :close @14:36:45.58 SS.reconfigure :default @14:36:45.62 SS.hold @14:36:45.83 CTV.dial :intake @14:36:46.07 SSV.dial CBV @14:36:46.11
CBV.dial SSV @14:36:46.87 Allowing 130s for 25.0ml of remaining 1000.0ml @14:36:46.88 Intake.select :open @14:36:49.05 Intake @14.8psia, vacuum from 9.3 to 12.3psi @14:36:49.07 SS.reconfigure :inhaling 2.20ml/s @14:36:49.11 SS.seek 25ml @14:36:56.79 Vacuum too high -- waiting up to 40 seconds @14:36:56.80 SS.reconfigure :exhale @14:36:56.85 SS.hold @14:37:00.19 Intake @13.2psia, vacuum from 7.7 to 10.7psi @14:37:00.21 SS.reconfigure :inhaling 1.76ml/s @14:37:00.26 SS.seek 25ml @14:37:06.47 Vacuum too high -- waiting up to 40 seconds @14:37:06.49 SS.reconfigure :exhale @14:37:06.54 SS.hold @14:37:12.08 Intake @14.5psia, vacuum from 9.0 to 12.0psi @14:37:12.09 SS.reconfigure :inhaling 1.41ml/s @14:37:12.14 SS.seek 25ml @14:37:16.56 Stroke completed -- waiting for pressure to equalize ... @14:37:16.57 SS.reconfigure :exhale @14:37:16.61 SS.hold @14:37:20.76 Intake.select :close @14:37:20.91 SSV.dial :exhaust @14:37:21.67 Exhaust.select :open @14:37:21.78 SS.seek 0ml @14:37:30.50 Exhaust.select :close @14:37:30.61 SSV.dial CBV @14:37:30.65
CBV.dial SSV @14:37:31.39 Allowing 130s for 25.0ml of remaining 975.0ml @14:37:31.40 Intake.select :open @14:37:33.56 Intake @14.8psia, vacuum from 9.3 to 12.3psi @14:37:33.58 SS.reconfigure :inhaling 1.41ml/s @14:37:33.63 SS.seek 25ml @14:37:50.16 Vacuum too high -- waiting up to 40 seconds @14:37:50.17 SS.reconfigure :exhale @14:37:50.22 SS.hold @14:37:55.45 Intake @13.9psia, vacuum from 8.4 to 11.4psi @14:37:55.47 SS.reconfigure :inhaling 1.13ml/s @14:37:55.51 SS.seek 25ml @14:38:01.18 Stroke completed -- waiting for pressure to equalize ... @14:38:01.19 SS.reconfigure :exhale @14:38:01.24 SS.hold @14:38:06.77 Intake.select :close @14:38:06.93 SSV.dial :exhaust @14:38:07.68 Exhaust.select :open @14:38:07.80 SS.seek 0ml @14:38:16.56 Exhaust.select :close @14:38:16.67 SSV.dial CBV @14:38:16.70
CBV.dial SSV @14:38:17.47 Allowing 130s for 25.0ml of remaining 950.0ml @14:38:17.48 Intake.select :open @14:38:19.66 Intake @14.8psia, vacuum from 9.3 to 12.3psi @14:38:19.68 SS.reconfigure :inhaling 1.13ml/s @14:38:19.73 SS.seek 25ml @14:38:44.52 Stroke completed -- waiting for pressure to equalize ... @14:38:44.54 SS.reconfigure :exhale @14:38:44.59 SS.hold @14:38:53.33 Intake.select :close @14:38:53.49 SSV.dial :exhaust @14:38:54.25 Exhaust.select :open @14:38:54.36 SS.seek 0ml @14:39:03.32 Exhaust.select :close @14:39:03.43 SSV.dial CBV @14:39:03.46
CBV.dial SSV @14:39:04.23 Allowing 130s for 25.0ml of remaining 925.0ml @14:39:04.24 Intake.select :open @14:39:06.40 Intake @14.9psia, vacuum from 9.4 to 12.4psi @14:39:06.42 SS.reconfigure :inhaling 1.13ml/s @14:39:06.47 SS.seek 25ml @14:39:30.88 Stroke completed -- waiting for pressure to equalize ... @14:39:30.89 SS.reconfigure :exhale @14:39:30.94 SS.hold @14:39:40.61 Intake.select :close @14:39:40.76 SSV.dial :exhaust @14:39:41.53 Exhaust.select :open @14:39:41.65 SS.seek 0ml @14:39:50.46 Exhaust.select :close @14:39:50.57 SSV.dial CBV @14:39:50.60
CBV.dial SSV @14:39:51.39 Allowing 130s for 25.0ml of remaining 900.0ml @14:39:51.40 Intake.select :open @14:39:53.56 Intake @14.8psia, vacuum from 9.3 to 12.3psi @14:39:53.58 SS.reconfigure :inhaling 1.13ml/s @14:39:53.63 SS.seek 25ml @14:40:17.00 Vacuum too high -- waiting up to 40 seconds @14:40:17.01 SS.reconfigure :exhale @14:40:17.07 SS.hold @14:40:22.84 Intake @14.0psia, vacuum from 8.5 to 11.5psi @14:40:22.86 SS.reconfigure :inhaling 0.91ml/s @14:40:22.90 SS.seek 25ml @14:40:24.76 Stroke completed -- waiting for pressure to equalize ... @14:40:24.77 SS.reconfigure :exhale @14:40:24.81 SS.hold @14:40:30.08 Intake.select :close @14:40:30.24 SSV.dial :exhaust @14:40:31.01 Exhaust.select :open @14:40:31.12 SS.seek 0ml @14:40:39.88 Exhaust.select :close @14:40:39.99 SSV.dial CBV @14:40:40.02
CBV.dial SSV @14:40:40.81 Allowing 130s for 25.0ml of remaining 875.0ml @14:40:40.82 Intake.select :open @14:40:42.98 Intake @14.8psia, vacuum from 9.3 to 12.3psi @14:40:43.00 SS.reconfigure :inhaling 0.91ml/s @14:40:43.05 SS.seek 25ml @14:41:13.19 Stroke completed -- waiting for pressure to equalize ... @14:41:13.20 SS.reconfigure :exhale @14:41:13.25 SS.hold @14:41:22.93 Intake.select :close @14:41:23.09 SSV.dial :exhaust @14:41:23.86 Exhaust.select :open @14:41:23.97 SS.seek 0ml @14:41:32.99 Exhaust.select :close @14:41:33.10 SSV.dial CBV @14:41:33.12
CBV.dial SSV @14:41:33.90 Allowing 130s for 25.0ml of remaining 850.0ml @14:41:33.91 Intake.select :open @14:41:36.08 Intake @14.8psia, vacuum from 9.3 to 12.3psi @14:41:36.10 SS.reconfigure :inhaling 0.91ml/s @14:41:36.14 SS.seek 25ml @14:42:05.58 Stroke completed -- waiting for pressure to equalize ... @14:42:05.61 SS.reconfigure :exhale @14:42:05.72 SS.hold @14:42:17.16 Intake.select :close @14:42:17.32 SSV.dial :exhaust @14:42:18.09 Exhaust.select :open @14:42:18.20 SS.seek 0ml @14:42:27.27 Exhaust.select :close @14:42:27.38 SSV.dial CBV @14:42:27.41
CBV.dial SSV @14:42:28.18 Allowing 130s for 25.0ml of remaining 825.0ml @14:42:28.19 Intake.select :open @14:42:30.35 Intake @14.8psia, vacuum from 9.3 to 12.3psi @14:42:30.37 SS.reconfigure :inhaling 0.91ml/s @14:42:30.42 SS.seek 25ml @14:42:59.89 Stroke completed -- waiting for pressure to equalize ... @14:42:59.90 SS.reconfigure :exhale @14:42:59.95 SS.hold @14:43:12.61 Intake.select :close @14:43:12.77 SSV.dial :exhaust @14:43:13.54 Exhaust.select :open @14:43:13.65 SS.seek 0ml @14:43:22.69 Exhaust.select :close @14:43:22.81 SSV.dial CBV @14:43:22.83
CBV.dial SSV @14:43:23.62 Allowing 130s for 25.0ml of remaining 800.0ml @14:43:23.63 Intake.select :open @14:43:25.80 Intake @14.8psia, vacuum from 9.3 to 12.3psi @14:43:25.81 SS.reconfigure :inhaling 0.91ml/s @14:43:25.86 SS.seek 25ml @14:43:56.14 Stroke completed -- waiting for pressure to equalize ... @14:43:56.15 SS.reconfigure :exhale @14:43:56.20 SS.hold @14:44:11.01 Intake.select :close @14:44:11.16 SSV.dial :exhaust @14:44:11.93 Exhaust.select :open @14:44:12.04 SS.seek 0ml @14:44:20.79 Exhaust.select :close @14:44:20.90 SSV.dial CBV @14:44:20.92
CBV.dial SSV @14:44:21.71 Allowing 130s for 25.0ml of remaining 775.0ml @14:44:21.72 Intake.select :open @14:44:23.88 Intake @14.8psia, vacuum from 9.3 to 12.3psi @14:44:23.90 SS.reconfigure :inhaling 0.91ml/s @14:44:23.95 SS.seek 25ml @14:44:46.28 Vacuum too high -- waiting up to 40 seconds @14:44:46.29 SS.reconfigure :exhale @14:44:46.34 SS.hold @14:44:53.29 Intake @14.3psia, vacuum from 8.8 to 11.8psi @14:44:53.31 SS.reconfigure :inhaling 0.73ml/s @14:44:53.35 SS.seek 25ml @14:45:03.14 Stroke completed -- waiting for pressure to equalize ... @14:45:03.15 SS.reconfigure :exhale @14:45:03.20 SS.hold @14:45:12.95 Intake.select :close @14:45:13.10 SSV.dial :exhaust @14:45:13.86 Exhaust.select :open @14:45:13.98 SS.seek 0ml @14:45:22.96 Exhaust.select :close @14:45:23.07 SSV.dial CBV @14:45:23.09
CBV.dial SSV @14:45:23.89 Allowing 130s for 25.0ml of remaining 750.0ml @14:45:23.91 Intake.select :open @14:45:26.07 Intake @14.7psia, vacuum from 9.2 to 12.2psi @14:45:26.09 SS.reconfigure :inhaling 0.73ml/s @14:45:26.14 SS.seek 25ml @14:46:02.06 Stroke completed -- waiting for pressure to equalize ... @14:46:02.07 SS.reconfigure :exhale @14:46:02.12 SS.hold @14:46:18.37 Intake.select :close @14:46:18.53 SSV.dial :exhaust @14:46:19.30 Exhaust.select :open @14:46:19.41 SS.seek 0ml @14:46:28.45 Exhaust.select :close @14:46:28.57 SSV.dial CBV @14:46:28.59
CBV.dial SSV @14:46:29.38 Allowing 130s for 25.0ml of remaining 725.0ml @14:46:29.39 Intake.select :open @14:46:31.55 Intake @14.6psia, vacuum from 9.1 to 12.1psi @14:46:31.57 SS.reconfigure :inhaling 0.73ml/s @14:46:31.62 SS.seek 25ml @14:47:06.93 Vacuum too high -- waiting up to 40 seconds @14:47:06.94 SS.reconfigure :exhale @14:47:06.99 SS.hold @14:47:15.66 Intake @14.1psia, vacuum from 8.6 to 11.6psi @14:47:15.67 SS.reconfigure :inhaling 0.59ml/s @14:47:15.72 SS.seek 25ml @14:47:16.78 Stroke completed -- waiting for pressure to equalize ... @14:47:16.79 SS.reconfigure :exhale @14:47:16.84 SS.hold @14:47:24.45 Intake.select :close @14:47:24.60 SSV.dial :exhaust @14:47:25.36 Exhaust.select :open @14:47:25.47 SS.seek 0ml @14:47:34.48 Exhaust.select :close @14:47:34.59 SSV.dial CBV @14:47:34.62
CBV.dial SSV @14:47:35.41 Allowing 130s for 25.0ml of remaining 700.0ml @14:47:35.42 Intake.select :open @14:47:37.58 Intake @14.8psia, vacuum from 9.3 to 12.3psi @14:47:37.60 SS.reconfigure :inhaling 0.59ml/s @14:47:37.65 SS.seek 25ml @14:48:22.23 Stroke completed -- waiting for pressure to equalize ... @14:48:22.24 SS.reconfigure :exhale @14:48:22.29 SS.hold @14:48:36.61 Intake.select :close @14:48:36.76 SSV.dial :exhaust @14:48:37.53 Exhaust.select :open @14:48:37.64 SS.seek 0ml @14:48:46.40 Exhaust.select :close @14:48:46.52 SSV.dial CBV @14:48:46.54
CBV.dial SSV @14:48:47.33 Allowing 130s for 25.0ml of remaining 675.0ml @14:48:47.34 Intake.select :open @14:48:49.51 Intake @14.6psia, vacuum from 9.1 to 12.1psi @14:48:49.52 SS.reconfigure :inhaling 0.59ml/s @14:48:49.57 SS.seek 25ml @14:49:34.53 Stroke completed -- waiting for pressure to equalize ... @14:49:34.54 SS.reconfigure :exhale @14:49:34.58 SS.hold @14:49:52.94 Intake.select :close @14:49:53.10 SSV.dial :exhaust @14:49:53.86 Exhaust.select :open @14:49:54.40 SS.seek 0ml @14:50:03.42 Exhaust.select :close @14:50:03.53 SSV.dial CBV @14:50:03.57
CBV.dial SSV @14:50:04.31 Allowing 130s for 25.0ml of remaining 650.0ml @14:50:04.32 Intake.select :open @14:50:06.48 Intake @14.8psia, vacuum from 9.3 to 12.3psi @14:50:06.50 SS.reconfigure :inhaling 0.59ml/s @14:50:06.55 SS.seek 25ml @14:50:50.95 Stroke completed -- waiting for pressure to equalize ... @14:50:50.96 SS.reconfigure :exhale @14:50:51.00 SS.hold @14:51:13.46 Intake.select :close @14:51:13.62 SSV.dial :exhaust @14:51:14.61 Exhaust.select :open @14:51:14.72 SS.seek 0ml @14:51:23.81 Exhaust.select :close @14:51:23.92 SSV.dial CBV @14:51:23.95
CBV.dial SSV @14:51:24.70 Allowing 130s for 25.0ml of remaining 625.0ml @14:51:24.71 Intake.select :open @14:51:26.87 Intake @14.6psia, vacuum from 9.1 to 12.1psi @14:51:26.89 SS.reconfigure :inhaling 0.59ml/s @14:51:26.94 SS.seek 25ml @14:52:05.71 Vacuum too high -- waiting up to 40 seconds @14:52:05.72 SS.reconfigure :exhale @14:52:05.77 SS.hold @14:52:18.54 Intake @14.7psia, vacuum from 9.2 to 12.2psi @14:52:18.55 SS.reconfigure :inhaling 0.47ml/s @14:52:18.60 SS.seek 25ml @14:52:25.51 Stroke completed -- waiting for pressure to equalize ... @14:52:25.52 SS.reconfigure :exhale @14:52:25.56 SS.hold @14:52:40.02 Intake.select :close @14:52:40.17 SSV.dial :exhaust @14:52:40.92 Exhaust.select :open @14:52:41.03 SS.seek 0ml @14:52:49.77 Exhaust.select :close @14:52:49.89 SSV.dial CBV @14:52:49.91
CBV.dial SSV @14:52:50.68 Allowing 130s for 25.0ml of remaining 600.0ml @14:52:50.69 Intake.select :open @14:52:52.85 Intake @14.7psia, vacuum from 9.2 to 12.2psi @14:52:52.87 SS.reconfigure :inhaling 0.47ml/s @14:52:52.92 SS.seek 25ml @14:53:48.57 Stroke completed -- waiting for pressure to equalize ... @14:53:48.58 SS.reconfigure :exhale @14:53:48.63 SS.hold @14:54:11.12 Intake.select :close @14:54:11.27 SSV.dial :exhaust @14:54:12.02 Exhaust.select :open @14:54:12.14 SS.seek 0ml @14:54:20.96 Exhaust.select :close @14:54:21.07 SSV.dial CBV @14:54:21.09
CBV.dial SSV @14:54:21.87 Allowing 130s for 25.0ml of remaining 575.0ml @14:54:21.88 Intake.select :open @14:54:24.05 Intake @14.7psia, vacuum from 9.2 to 12.2psi @14:54:24.06 SS.reconfigure :inhaling 0.47ml/s @14:54:24.11 SS.seek 25ml @14:55:19.68 Stroke completed -- waiting for pressure to equalize ... @14:55:19.69 SS.reconfigure :exhale @14:55:19.73 SS.hold @14:55:46.27 Intake.select :close @14:55:46.43 SSV.dial :exhaust @14:55:47.19 Exhaust.select :open @14:55:47.30 SS.seek 0ml @14:55:56.30 Exhaust.select :close @14:55:56.41 SSV.dial CBV @14:55:56.44
CBV.dial SSV @14:55:57.19 Allowing 130s for 25.0ml of remaining 550.0ml @14:55:57.21 Intake.select :open @14:55:59.38 Intake @14.7psia, vacuum from 9.2 to 12.2psi @14:55:59.39 SS.reconfigure :inhaling 0.47ml/s @14:55:59.44 SS.seek 25ml @14:56:48.51 Vacuum too high -- waiting up to 40 seconds @14:56:48.52 SS.reconfigure :exhale @14:56:48.58 SS.hold @14:57:05.01 Intake @15.1psia, vacuum from 9.6 to 12.6psi @14:57:05.02 SS.reconfigure :inhaling 0.38ml/s @14:57:05.07 SS.seek 25ml @14:57:12.77 Stroke completed -- waiting for pressure to equalize ... @14:57:12.78 SS.reconfigure :exhale @14:57:12.82 SS.hold @14:57:31.17 Intake.select :close @14:57:31.33 SSV.dial :exhaust @14:57:32.07 Exhaust.select :open @14:57:32.18 SS.seek 0ml @14:57:40.92 Exhaust.select :close @14:57:41.04 SSV.dial CBV @14:57:41.06
CBV.dial SSV @14:57:41.90 Allowing 130s for 25.0ml of remaining 525.0ml @14:57:41.91 Intake.select :open @14:57:44.08 Intake @14.7psia, vacuum from 9.2 to 12.2psi @14:57:44.10 SS.reconfigure :inhaling 0.38ml/s @14:57:44.15 SS.seek 25ml @14:58:54.24 Stroke completed -- waiting for pressure to equalize ... @14:58:54.25 SS.reconfigure :exhale @14:58:54.29 SS.hold @14:59:22.92 Intake.select :close @14:59:23.07 SSV.dial :exhaust @14:59:23.83 Exhaust.select :open @14:59:23.94 SS.seek 0ml @14:59:32.75 Exhaust.select :close @14:59:33.28 SSV.dial CBV @14:59:33.30
CBV.dial SSV @14:59:34.08 Allowing 130s for 25.0ml of remaining 500.0ml @14:59:34.09 Intake.select :open @14:59:36.26 Intake @14.6psia, vacuum from 9.1 to 12.1psi @14:59:36.28 SS.reconfigure :inhaling 0.38ml/s @14:59:36.33 SS.seek 25ml @15:00:46.51 Stroke completed -- waiting for pressure to equalize ... @15:00:46.52 SS.reconfigure :exhale @15:00:46.57 SS.hold @15:01:19.71 Intake.select :close @15:01:19.89 SSV.dial :exhaust @15:01:20.66 Exhaust.select :open @15:01:20.77 SS.seek 0ml @15:01:29.59 Exhaust.select :close @15:01:29.71 SSV.dial CBV @15:01:29.73
CBV.dial SSV @15:01:30.52 Allowing 130s for 25.0ml of remaining 475.0ml @15:01:30.53 Intake.select :open @15:01:32.74 Intake @14.7psia, vacuum from 9.2 to 12.2psi @15:01:32.76 SS.reconfigure :inhaling 0.38ml/s @15:01:32.81 SS.seek 25ml @15:02:34.10 Vacuum too high -- waiting up to 40 seconds @15:02:34.11 SS.reconfigure :exhale @15:02:34.17 SS.hold @15:02:52.66 Intake @15.1psia, vacuum from 9.6 to 12.6psi @15:02:52.67 SS.reconfigure :inhaling 0.31ml/s @15:02:52.72 SS.seek 25ml @15:03:04.10 Stroke completed -- waiting for pressure to equalize ... @15:03:04.11 SS.reconfigure :exhale @15:03:04.17 SS.hold @15:03:26.98 Intake.select :close @15:03:27.13 SSV.dial :exhaust @15:03:27.90 Exhaust.select :open @15:03:28.02 SS.seek 0ml @15:03:36.79 Exhaust.select :close @15:03:36.90 SSV.dial CBV @15:03:36.93
CBV.dial SSV @15:03:37.70 Allowing 130s for 25.0ml of remaining 450.0ml @15:03:37.71 Intake.select :open @15:03:39.87 Intake @14.7psia, vacuum from 9.2 to 12.2psi @15:03:39.89 SS.reconfigure :inhaling 0.31ml/s @15:03:39.94 SS.seek 25ml @15:05:09.95 Stroke completed -- waiting for pressure to equalize ... @15:05:09.96 SS.reconfigure :exhale @15:05:10.00 SS.hold @15:05:42.96 Intake.select :close @15:05:43.11 SSV.dial :exhaust @15:05:43.88 Exhaust.select :open @15:05:43.99 SS.seek 0ml @15:05:53.01 Exhaust.select :close @15:05:53.12 SSV.dial CBV @15:05:53.14
CBV.dial SSV @15:05:53.92 Allowing 130s for 25.0ml of remaining 425.0ml @15:05:53.93 Intake.select :open @15:05:56.09 Intake @14.8psia, vacuum from 9.3 to 12.3psi @15:05:56.46 SS.reconfigure :inhaling 0.31ml/s @15:05:56.58 SS.seek 25ml @15:07:26.51 Stroke completed -- waiting for pressure to equalize ... @15:07:26.52 SS.reconfigure :exhale @15:07:26.56 SS.hold @15:08:03.94 SS.reconfigure :default @15:08:03.98 SS.hold @15:08:04.48 Intake.select :close @15:08:04.64 ShallowSampler::Clogged in MAIN -- Filtering too Slowly after 600.0ml @15:08:04.65 SSV.dial :exhaust @15:08:05.41 Exhaust.select :open @15:08:05.53 SS.reconfigure :exhale @15:08:05.58 SS.seek 0ml @15:08:14.58 Exhaust.select :close @15:08:14.70 Sampled 600.0ml @15:08:14.71 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @15:08:14.72 Intake.select :close @15:08:14.79 CSV.dial :waste @15:08:14.91 SSV.dial CBV,avoiding: :exhaust @15:08:14.94
CBV.dial SSV @15:08:15.06 CS.seek 0ml @15:08:15.15 CSV.dial :air @15:08:15.88 CS.seek 10ml @15:08:16.81
CTV.dial :air @15:08:17.57 delay 15 seconds @15:08:32.59 SSV.dial :spit,avoiding: :exhaust @15:08:33.90 CTV.dial CSV,avoiding: :intake @15:08:33.94
SS.seek 0ml @15:08:34.07 SSV.dial CBV,avoiding: :exhaust @15:08:40.54 SSV.dial between CBV and plug @15:08:41.03 CBV.dial between SSV and waste2 @15:08:41.43 CTV.dial CSV,avoiding: :air @15:08:41.45
CSV.dial CTV @15:08:41.94 CS.reconfigure :pressurize @15:08:41.99 CS.seek 0ml @15:09:00.57 BubblePoint: Intake @24.1psia, Outlet @0.2psia @15:09:00.58 CBV.dial SSV @15:09:00.93 SS.reconfigure :default @15:09:00.98 SS.seek 10ml @15:09:06.48 delay 15 seconds @15:09:21.61 Evacuated: Intake @23.8psia, Outlet @24.0psia @15:09:21.62 SSV.dial :spit,avoiding: :exhaust @15:09:22.67 SS.reconfigure :exhale @15:09:22.72 SS.seek 0ml @15:09:27.92 SS.reconfigure :fast @15:09:27.97 SS.seek 5ml @15:09:30.71 SSV.dial CBV,avoiding: :exhaust @15:09:30.74
CBV.dial SSV @15:09:32.09 CTV.dial :air,avoiding: :intake @15:09:33.41 CSV.dial CTV @15:09:33.46
SS.seek 10ml @15:09:34.62 CSV.dial :air @15:09:35.05 CS.reconfigure :fast @15:09:35.52 CS.seek 0ml @15:09:35.95
SSV.dial :spit,avoiding: :exhaust @15:09:37.26 SS.reconfigure :exhale @15:09:37.31 SS.seek 0ml @15:09:42.53 SS.reconfigure :fast @15:09:42.58 SS.seek 5ml @15:09:43.16 CS.stop @15:09:45.48 SSV.dial CBV,avoiding: :exhaust @15:09:45.51
CBV.dial SSV @15:09:47.86 SSV.dial :spit,avoiding: :exhaust @15:09:49.18 SS.reconfigure :default @15:09:49.23 SS.seek 0ml @15:09:52.32 SS.stop @15:09:52.36 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @15:09:52.37 Intake.select :close @15:09:52.44 CSV.dial :waste @15:09:52.57 SSV.dial CBV,avoiding: :exhaust @15:09:52.59
CBV.dial SSV @15:09:53.24 CS.seek 0ml @15:09:53.31 CSV.dial :air @15:09:53.96
CTV.dial :air @15:09:54.04 delay 15 seconds @15:09:54.08 CS.seek 10ml @15:10:09.06
SSV.dial :spit,avoiding: :exhaust @15:10:10.39 CTV.dial CSV,avoiding: :intake @15:10:10.42
SS.reconfigure :exhale @15:10:10.51 SS.seek 0ml @15:10:10.96 SSV.dial CBV,avoiding: :exhaust @15:10:18.69 SSV.dial between CBV and plug @15:10:19.18 CBV.dial between SSV and waste2 @15:10:19.59 CSV.dial between air and CTV @15:10:19.90 CS.seek 7.742ml @15:10:25.91 CTV.dial CSV,avoiding: :air @15:10:25.94
CSV.dial CTV @15:10:26.44 CS.reconfigure :pressurize @15:10:26.49 CS.seek 0ml @15:10:42.82 BubblePoint: Intake @24.3psia, Outlet @3.0psia @15:10:42.83 CBV.dial SSV @15:10:43.16 SS.reconfigure :default @15:10:43.21 SS.seek 10ml @15:10:48.80 delay 15 seconds @15:11:03.93 Evacuated: Intake @23.9psia, Outlet @24.3psia @15:11:03.95 SSV.dial :spit,avoiding: :exhaust @15:11:04.99 SS.reconfigure :exhale @15:11:05.04 SS.seek 0ml @15:11:10.52 SS.reconfigure :fast @15:11:10.56 SS.seek 5ml @15:11:13.35 SSV.dial CBV,avoiding: :exhaust @15:11:13.38
CBV.dial SSV @15:11:14.71 CTV.dial :air,avoiding: :intake @15:11:16.03 CSV.dial CTV @15:11:16.07
SS.seek 10ml @15:11:17.20 CSV.dial :air @15:11:17.64 CS.reconfigure :fast @15:11:17.69 CS.seek 0ml @15:11:18.54
SSV.dial :spit,avoiding: :exhaust @15:11:19.85 SS.reconfigure :exhale @15:11:19.90 SS.seek 0ml @15:11:22.19 CS.stop @15:11:24.95
SS.reconfigure :fast @15:11:25.00 SS.seek 5ml @15:11:27.97 SSV.dial CBV,avoiding: :exhaust @15:11:28.00
CBV.dial SSV @15:11:30.34 SSV.dial :spit,avoiding: :exhaust @15:11:31.66 SS.reconfigure :default @15:11:31.71 SS.seek 0ml @15:11:34.86 SS.stop @15:11:34.90 Lysing: Adding 2.0ml AqMeOH @15:11:34.92 CBV.dial between CSV and waste1,avoiding: :DBV @15:11:36.41 CTV.dial CSV @15:11:37.72 CSV.dial CSR @15:11:38.51 Pulling 1ml of CSR.AqMeOH @15:11:38.52 CSR.select :AqMeOH @15:11:38.63 CS.reconfigure :default @15:11:38.68 CS.seek 1ml @15:11:42.16 delay 5 seconds @15:11:47.17 CSR.select :air @15:11:47.34 CS.seek 6ml @15:12:00.97 delay 2 seconds @15:12:02.98 CSV.dial :waste2 @15:12:03.55 CS.reconfigure :fast @15:12:03.60 CS.seek 0ml @15:12:17.94 CSV.dial CSR @15:12:18.38 Pulling 2ml of CSR.AqMeOH CSR.select :AqMeOH @15:12:18.50 CS.reconfigure :default @15:12:18.55 CS.seek 2ml @15:12:24.53 delay 5 seconds @15:12:29.55 CSR.select :air @15:12:29.72 CS.seek 5.001ml @15:12:38.19 delay 2 seconds @15:12:40.20 CSV.dial CTV @15:12:40.64 CS.seek 1ml @15:12:51.83 Before lysing Collection Puck Heater at 16.7C/29.6psia @15:12:51.85 Heating Puck.from 2, :sh1 in Collection Clamp to 70C for 10:00 @15:12:52.29 CH.seek 70C,8:00 @15:15:01.88 CH.hold 10:00,70C @15:15:02.09 Begun lysing Collection Puck Heater at 68.1C/35.8psia @15:25:02.11 Ended lysing Collection Puck Heater at 70C/34.9psia @15:25:02.12 CS.seek 0ml @15:25:05.74 CTV.dial between spare and air,avoiding: :intake @15:25:06.80 CSV.dial :air @15:25:07.24 CS.seek 5ml @15:25:20.80 CSV.dial CTV @15:25:21.25 delay 2 seconds @15:25:23.27 CSV.dial :air @15:25:23.72 delay 2 seconds @15:25:25.74 CSV.dial CTV @15:25:26.19 delay 2 seconds @15:25:28.21 CSV.dial :air @15:25:28.66 delay 2 seconds @15:25:30.68 CS.seek 0ml @15:25:44.32 Puck @80.4psia after final lysis pressurization @15:25:44.33 Recovering Lysate into Collection syringe @15:25:44.34 CSV.dial CBV @15:25:45.36 CBV.dial CSV @15:25:45.73 CS.seek 5.001ml @15:25:59.30 delay 5 seconds @15:26:04.32 CTV.dial :air @15:26:04.86 CS.seek 7ml @15:26:10.86 delay 5 seconds @15:26:15.88 CSV.dial :air2 @15:26:16.32 CS.seek 10ml @15:26:24.86 CSV.dial between air and CTV,avoiding: CBV @15:26:26.02 Lysing Complete @15:26:26.09 Storing sh1 puck in Collection Clamp to tube 1 @15:26:26.27 SC.reconfigure :tight @15:26:26.31 SC.seek 1 @15:26:29.87 SC.configure :limp @15:26:30.08 Elbow.reconfigure :tight @15:26:30.13 Elbow.seek 1 @15:26:31.95 Forearm.reconfigure :tight @15:26:31.99 Forearm.seek 1 + 450 counts @15:26:33.13 SE.reconfigure :pick @15:26:33.19 SE.seek :beamBreak @15:26:34.81 SE.configure :default @15:26:34.98 Elbow.reconfigure :limp @15:26:35.03 Forearm.reconfigure :loose @15:26:35.08 Forearm.seek 1 @15:26:35.42 Elbow.hold @15:26:35.48 Elbow.reconfigure :tight @15:26:35.58 SE.seek 23.04 Pucks below Top Plate @15:26:36.10 Forearm.force -20 @15:26:36.15 Forearm.seek 1 + 299 counts @15:26:36.43 Forearm.reconfigure :tight @15:26:36.48 Forearm.seek 1 + 450 counts @15:26:36.67 SE.seek 24.04 Pucks below Top Plate @15:26:37.27 Forearm.reconfigure :loose @15:26:37.32 Forearm.seek :retract @15:26:38.25 Elbow.seek CC @15:26:39.96 Elbow.reconfigure :loose @15:26:40.06 Forearm.seek CC @15:26:41.01 Hand.close @15:26:41.58 CC.seek :open @15:26:46.31 Forearm.seek :retract @15:26:47.31 Elbow.reconfigure :tight @15:26:47.36 Elbow.seek 1 @15:26:49.12 Elbow.reconfigure :limp @15:26:49.18 Forearm.seek 1 @15:26:50.21 Elbow.hold @15:26:50.28 Elbow.reconfigure :tight @15:26:50.33 Hand.open @15:26:51.51 Forearm.force -20 @15:26:51.56 Forearm.seek 1 + 319 counts @15:26:51.87 Forearm.reconfigure :tight @15:26:51.92 Forearm.seek 1 + 450 counts @15:26:52.19 SE.seek :down @15:26:53.27 SE.stop @15:26:53.40
Forearm.reconfigure :loose @15:26:53.45 Forearm.seek :retract @15:26:54.36 Tranferring DA @15:26:54.37 Beginning samplePassC @15:26:54.38 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @15:26:54.58 Elbow.seek FlushPuck::Garage @15:26:56.41 Elbow.reconfigure :loose @15:26:56.53 Forearm.seek :garage @15:26:57.53 Hand.close @15:26:58.32 Forearm.seek :retract @15:26:59.33 Elbow.reconfigure :tight @15:26:59.38 Elbow.seek CC @15:27:02.29 Elbow.reconfigure :loose @15:27:02.39 Forearm.reconfigure :tight @15:27:02.44 Forearm.seek CC @15:27:03.66 CC.seek :closed @15:27:08.18 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.36 @15:27:08.69 Hand.open @15:27:09.39 Forearm.reconfigure :loose @15:27:09.44 Forearm.seek :retract @15:27:10.39 PSV.dial PSR @15:27:11.81 Pulling 1.5ml of PSR.flush @15:27:11.82 PSR.select :flush @15:27:11.94 PS.seek 1.5ml @15:27:28.05 delay 5 seconds @15:27:33.07 PSV.dial :waste1 @15:27:33.54 PS.seek 0ml @15:27:49.71 PSV.dial PSR @15:27:50.18 PSR.select :air @15:27:50.30 PS.seek 2.5ml @15:28:16.62 PRV.dial CBV,avoiding: :ab2 @15:28:16.66
CBV.dial PRV @15:28:18.41 PSV.dial :mixloop @15:28:19.44 CTV.dial :air,avoiding: :intake @15:28:19.67 PTV.dial :PRVmixing @15:28:20.99 PS.seek 0ml @15:28:47.52 PSV.dial :air @15:28:47.99 PS.seek 2.5ml @15:29:14.30 PSV.dial :mixloop @15:29:14.77 PS.seek 0ml @15:29:41.14 PSV.dial :air @15:29:41.62 PS.seek 2.5ml @15:30:08.02 PSV.dial :mixloop @15:30:08.49 PS.seek 0ml @15:30:34.92 Garaging FlushPuck in Collection Clamp @15:30:35.00 Elbow.reconfigure :tight @15:30:35.04 Elbow.seek CC @15:30:35.36 Elbow.reconfigure :loose @15:30:35.47 Forearm.seek CC @15:30:36.51 Hand.close @15:30:37.07 CC.seek :open @15:30:41.69 Forearm.seek :retract @15:30:42.70 Elbow.reconfigure :tight @15:30:42.75 Elbow.seek :garage @15:30:46.00 Elbow.reconfigure :loose @15:30:46.11 Forearm.reconfigure :tight @15:30:46.16 Forearm.seek :garage @15:30:47.23 Hand.open @15:30:47.89 Forearm.reconfigure :loose @15:30:47.94 Forearm.seek :retract @15:30:48.95 PSV.dial :mixloop @15:30:49.02 PTV.dial :PRVmixing @15:30:49.10 PRV.dial CBV @15:30:49.13
CBV.dial PRV @15:30:49.27 CTV.dial :air,avoiding: :intake @15:30:49.34 Loading lyfil puck from tube 2 into Collection Clamp @15:30:49.59 SC.reconfigure :tight @15:30:49.63 SC.seek 2 @15:30:53.21 SC.configure :limp @15:30:53.43 Elbow.reconfigure :tight @15:30:53.47 Elbow.seek 2 @15:30:55.38 Forearm.reconfigure :tight @15:30:55.43 Forearm.seek 2 + 450 counts @15:30:56.43 SE.reconfigure :pick @15:30:56.49 SE.seek :beamBreak @15:30:59.15 SE.configure :default @15:30:59.31 Elbow.seek 2 @15:30:59.50 Forearm.seek 2 + 300 counts @15:30:59.76 Forearm.reconfigure :loose @15:30:59.80 Hand.close @15:31:00.46 SE.seek :down @15:31:00.66
Forearm.seek :retract @15:31:02.04 Elbow.seek CC @15:31:02.78 SE.stop @15:31:03.68
Elbow.reconfigure :loose @15:31:03.79 Forearm.reconfigure :tight @15:31:03.83 Forearm.seek CC @15:31:05.15 CC.seek :closed @15:31:09.67 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.14 @15:31:09.68 Hand.open @15:31:10.38 Forearm.reconfigure :loose @15:31:10.43 Forearm.seek :retract @15:31:11.42 CTV.dial CSV,avoiding: :intake @15:31:12.75 CSV.dial CTV @15:31:13.08 PS.seek 2.5ml @15:31:39.42 CS.seek 4.501ml @15:31:54.42 delay 8 seconds @15:32:02.44 PSV.dial :air @15:32:02.91 delay 2 seconds @15:32:04.92 PSV.dial :waste2 @15:32:05.66 PS.seek 0ml @15:32:31.98 CBV.dial between PRV and waste1 @15:32:32.31 CSV.dial :air @15:32:32.78 CS.seek 10ml @15:32:47.73 CSV.dial CTV @15:32:48.19 CS.seek 0ml @15:33:14.79 CBV.dial :waste1 @15:33:15.10 delay 5 seconds @15:33:20.13 CTV.dial :air,avoiding: :intake @15:33:21.52 Storing lyfil puck in Collection Clamp to tube 1 @15:33:22.16 SC.reconfigure :tight @15:33:22.20 SC.seek 1 @15:33:25.80 SC.configure :limp @15:33:26.03 Elbow.reconfigure :tight @15:33:26.08 Elbow.seek 1 @15:33:27.86 Forearm.reconfigure :tight @15:33:27.91 Forearm.seek 1 + 450 counts @15:33:28.99 SE.reconfigure :pick @15:33:29.05 SE.seek :beamBreak @15:33:30.63 SE.configure :default @15:33:30.79 Elbow.reconfigure :limp @15:33:30.85 Forearm.reconfigure :loose @15:33:30.90 Forearm.seek 1 @15:33:31.18 Elbow.hold @15:33:31.24 Elbow.reconfigure :tight @15:33:31.34 SE.seek 24.04 Pucks below Top Plate @15:33:31.86 Forearm.force -20 @15:33:31.90 Forearm.seek 1 + 301 counts @15:33:32.19 Forearm.reconfigure :tight @15:33:32.24 Forearm.seek 1 + 450 counts @15:33:32.43 SE.seek 25.04 Pucks below Top Plate @15:33:33.03 Forearm.reconfigure :loose @15:33:33.08 Forearm.seek :retract @15:33:34.06 Elbow.seek CC @15:33:35.75 Elbow.reconfigure :loose @15:33:35.86 Forearm.seek CC @15:33:36.88 Hand.close @15:33:37.45 CC.seek :open @15:33:42.62 Forearm.seek :retract @15:33:43.59 Elbow.reconfigure :tight @15:33:43.64 Elbow.seek 1 @15:33:45.43 Elbow.reconfigure :limp @15:33:45.49 Forearm.seek 1 @15:33:46.54 Elbow.hold @15:33:46.61 Elbow.reconfigure :tight @15:33:46.66 Hand.open @15:33:47.44 Forearm.force -20 @15:33:47.49 Forearm.seek 1 + 315 counts @15:33:47.78 Forearm.reconfigure :tight @15:33:47.83 Forearm.seek 1 + 450 counts @15:33:48.10 SE.seek :down @15:33:49.11 SE.stop @15:33:49.27
Forearm.reconfigure :loose @15:33:49.32 Forearm.seek :retract @15:33:50.24 PSV.dial :air @15:33:50.99 PS.seek 2.5ml @15:34:17.29 delay 1 second @15:34:18.31 PSV.dial :mixloop @15:34:18.77 delay 1 second @15:34:19.78 PSV.dial :air @15:34:20.37 delay 1 second @15:34:21.39 PSV.dial :mixloop @15:34:21.84 delay 1 second @15:34:22.86 PSV.dial :air @15:34:23.33 delay 1 second @15:34:24.35 PS.seek 0ml @15:34:50.64 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @15:34:50.83 Elbow.seek FlushPuck::Garage @15:34:52.70 Elbow.reconfigure :loose @15:34:53.17 Forearm.seek :garage @15:34:54.24 Hand.close @15:34:54.94 Forearm.seek :retract @15:34:55.95 Elbow.reconfigure :tight @15:34:56.00 Elbow.seek PC @15:34:57.27 Elbow.reconfigure :loose @15:34:57.37 Forearm.reconfigure :tight @15:34:57.42 Forearm.seek PC @15:34:58.69 PC.seek :closed @15:35:03.36 Processing Clamp onSpring/offSpring for 16/26 samples, on/off torque 1.61 @15:35:03.43 Hand.open @15:35:04.13 Forearm.reconfigure :loose @15:35:04.18 Forearm.seek :retract @15:35:05.19 PRV.dial :air @15:35:05.64 PSV.dial :mixloop @15:35:06.17 PS.seek 0.3ml @15:35:10.14 PSV.dial PSR @15:35:11.19 Pulling 0.4ml of PSR.air @15:35:11.20 PSR.select :air @15:35:11.22 PS.seek 0.7ml @15:35:16.11 delay 3 seconds @15:35:19.12 PSR.select :air @15:35:19.15 PS.seek 2.5ml @15:35:38.36 delay 3 seconds @15:35:41.37 PSV.dial :mixloop @15:35:42.42 PTV.dial :mixing @15:35:43.18 PBV.dial :waste2 @15:35:44.14 PS.seek 0ml @15:36:10.48 Garaging FlushPuck in Processing Clamp @15:36:10.55 Elbow.reconfigure :tight @15:36:10.60 Elbow.seek PC @15:36:11.17 Elbow.reconfigure :loose @15:36:11.28 Forearm.seek PC @15:36:12.24 Hand.close @15:36:12.82 PC.seek :open @15:36:17.54 Forearm.seek :retract @15:36:18.49 Elbow.reconfigure :tight @15:36:18.54 Elbow.seek :garage @15:36:19.92 Elbow.reconfigure :loose @15:36:20.02 Forearm.reconfigure :tight @15:36:20.07 Forearm.seek :garage @15:36:21.03 Hand.open @15:36:21.75 Forearm.reconfigure :loose @15:36:21.80 Forearm.seek :retract @15:36:22.77 Loading da puck from tube 2 into Processing Clamp @15:36:23.01 SC.reconfigure :tight @15:36:23.06 SC.seek 2 @15:36:26.64 SC.configure :limp @15:36:26.86 Elbow.reconfigure :tight @15:36:26.90 Elbow.seek 2 @15:36:28.86 Forearm.reconfigure :tight @15:36:28.91 Forearm.seek 2 + 450 counts @15:36:29.96 SE.reconfigure :pick @15:36:30.02 SE.seek :beamBreak @15:36:32.82 SE.configure :default @15:36:32.98 Elbow.seek 2 @15:36:33.16 Forearm.seek 2 + 300 counts @15:36:33.43 Forearm.reconfigure :loose @15:36:33.47 Hand.close @15:36:34.08 SE.seek :down @15:36:34.73
Forearm.seek :retract @15:36:35.67 Elbow.seek PC @15:36:36.92 SE.stop @15:36:38.22
Elbow.reconfigure :loose @15:36:38.32 Forearm.reconfigure :tight @15:36:38.37 Forearm.seek PC @15:36:39.58 PC.seek :closed @15:36:44.21 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.64 @15:36:44.22 Hand.open @15:36:44.88 Forearm.reconfigure :loose @15:36:44.93 Forearm.seek :retract @15:36:45.90 Heating Puck.from 2, :da in Processing Clamp to 28C for 2:00:00 @15:36:45.92 PH.seek 28C,5:00 @15:37:27.59 PH.hold 2:00:00,28C @15:37:27.64 delay 1:30 @15:38:57.69 PSR.select :air @15:38:57.71 PSV.dial PSR @15:38:58.78 Pulling 0.5ml of PSR.wash PSR.select :wash @15:38:58.90 PS.seek 0.5ml @15:39:04.91 delay 5 seconds @15:39:09.93 PSR.select :air @15:39:10.06 PS.seek 2ml @15:39:26.19 PTV.dial :puck @15:39:26.95 PSV.dial PTV @15:39:27.71 PBV.dial :waste1 @15:39:29.12 PS.seek 1.5ml @15:39:35.04 delay 3 seconds @15:39:38.06 PBV.dial between PSV and waste1 @15:39:38.42 PS.seek 0ml @15:39:54.76 delay 5:00 @15:44:54.77 recoverPSRreagent08 @15:44:54.78 PTV.dial :puck @15:44:54.86 PBV.dial :waste1 @15:44:55.24 delay 30 seconds @15:45:25.26 PBV.dial between PSV and waste1 @15:45:25.63 PSR.select :air @15:45:25.64 PSV.dial PSR @15:45:26.41 PS.reconfigure :fast @15:45:26.46 PS.seek 2.5ml @15:45:50.65 PSV.dial PTV @15:45:51.42 delay 5 seconds @15:45:56.44 PSV.dial PSR @15:45:57.19 delay 5 seconds @15:46:02.21 PSV.dial PTV @15:46:02.97 delay 5 seconds @15:46:07.99 PSV.dial :air @15:46:08.77 PS.seek 0ml @15:46:32.28 PTV.dial :PRVmixing,via: PRV @15:46:33.61 PSV.dial :mixloop @15:46:34.08 PRV.dial :ab1 @15:46:35.28 PS.reconfigure :slow @15:46:35.33 PS.seek 0.15ml @15:46:38.87 delay 2 seconds @15:46:40.89 CBV.dial PRV @15:46:40.92
PRV.dial CBV @15:46:41.50 PS.seek 0.35ml @15:46:46.03 delay 2 seconds @15:46:48.05 CBV.dial between PRV and PSV @15:46:48.37 PRV.dial :air @15:46:48.83 PS.seek 0.9ml @15:47:00.62 PRV.dial :waste2 @15:47:01.38 PS.seek 0.5ml @15:47:10.00 addPMLReagent08 @15:47:10.01 PSV.dial :air @15:47:10.49 PS.reconfigure :default @15:47:10.54 PS.seek 2ml @15:47:26.65 PSV.dial :PML @15:47:27.12 PTV.dial :mixing @15:47:27.89 PBV.dial :waste1 @15:47:28.31 PS.seek 1.85ml @15:47:30.65 PBV.dial between PSV and waste1 @15:47:31.02 PS.seek 0ml @15:47:50.78 delay 2 seconds @15:47:52.80 Beginning samplePassC @15:47:52.81 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @15:47:52.99 Elbow.reconfigure :tight @15:47:53.05 Elbow.seek FlushPuck::Garage @15:47:54.41 Elbow.reconfigure :loose @15:47:54.52 Forearm.seek :garage @15:47:55.46 Hand.close @15:47:56.19 Forearm.seek :retract @15:47:57.15 Elbow.reconfigure :tight @15:47:57.20 Elbow.seek CC @15:48:00.27 Elbow.reconfigure :loose @15:48:00.37 Forearm.reconfigure :tight @15:48:00.42 Forearm.seek CC @15:48:02.06 CC.seek :closed @15:48:06.59 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.42 @15:48:06.66 Hand.open @15:48:07.37 Forearm.reconfigure :loose @15:48:07.42 Forearm.seek :retract @15:48:08.43 PSV.dial PSR @15:48:09.50 Pulling 1.5ml of PSR.flush @15:48:09.51 PSR.select :flush @15:48:09.63 PS.seek 1.5ml @15:48:25.72 delay 5 seconds @15:48:30.74 PSV.dial :waste1 @15:48:31.21 PS.seek 0ml @15:48:47.37 PSV.dial PSR @15:48:47.84 PSR.select :air @15:48:47.96 PS.seek 2.5ml @15:49:14.34 PRV.dial CBV,avoiding: :ab2 @15:49:14.37
CBV.dial PRV @15:49:16.00 PSV.dial :mixloop @15:49:17.04 CTV.dial :air,avoiding: :intake @15:49:17.12 PTV.dial :PRVmixing @15:49:17.91 PS.seek 0ml @15:49:44.18 PSV.dial :air @15:49:44.77 PS.seek 2.5ml @15:50:11.21 PSV.dial :mixloop @15:50:11.67 PS.seek 0ml @15:50:37.99 PSV.dial :air @15:50:38.47 PS.seek 2.5ml @15:51:04.87 PSV.dial :mixloop @15:51:05.34 PS.seek 0ml @15:51:31.68 Garaging FlushPuck in Collection Clamp @15:51:31.75 Elbow.reconfigure :tight @15:51:31.80 Elbow.seek CC @15:51:31.92 Elbow.reconfigure :loose @15:51:32.48 Forearm.seek CC @15:51:33.51 Hand.close @15:51:34.19 CC.seek :open @15:51:38.80 Forearm.seek :retract @15:51:39.76 Elbow.reconfigure :tight @15:51:39.81 Elbow.seek :garage @15:51:42.71 Elbow.reconfigure :loose @15:51:42.81 Forearm.reconfigure :tight @15:51:42.86 Forearm.seek :garage @15:51:43.80 Hand.open @15:51:44.49 Forearm.reconfigure :loose @15:51:44.54 Forearm.seek :retract @15:51:45.51 delayUntil "15:55:52.79" @15:51:45.54 Whole Cell Archive sampling 1000ml @15:51:45.56 Loading wcr puck from tube 2 into Collection Clamp @15:51:45.80 SC.reconfigure :tight @15:51:45.85 SC.seek 2 @15:51:46.18 SC.configure :limp @15:51:46.41 Elbow.reconfigure :tight @15:51:46.46 Elbow.seek 2 @15:51:48.38 Forearm.reconfigure :tight @15:51:48.43 Forearm.seek 2 + 450 counts @15:51:49.47 SE.reconfigure :pick @15:51:49.53 SE.seek :beamBreak @15:51:52.32 SE.configure :default @15:51:52.49 Elbow.seek 2 @15:51:52.68 Forearm.seek 2 + 300 counts @15:51:53.37 Forearm.reconfigure :loose @15:51:53.42 Hand.close @15:51:54.07 SE.seek :down @15:51:54.30 Forearm.seek :retract @15:51:55.28 Elbow.seek CC @15:51:56.53 SE.stop @15:51:56.94 Elbow.reconfigure :loose @15:51:57.05 Forearm.reconfigure :tight @15:51:57.10 Forearm.seek CC @15:51:58.34 CC.seek :closed @15:52:02.87 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.28 @15:52:02.89 Hand.open @15:52:03.62 Forearm.reconfigure :loose @15:52:03.67 Forearm.seek :retract @15:52:04.64 collectSample 1000ml w/bubble point of 35psi @15:52:04.66 Priming 7ml @15:52:04.67 Intake.select :close @15:52:04.68 Exhaust.select :close @15:52:04.69 SS.hold @15:52:04.90 Calibrating pressure sensors @15:52:04.92 CTV.dial :air @15:52:04.99 SSV.dial :spit,avoiding: :exhaust @15:52:05.09 SS.reconfigure :fast @15:52:05.14 SS.seek 5ml @15:52:07.96 SSV.dial CBV @15:52:07.99 CBV.dial SSV @15:52:09.33 delay 3.8 seconds @15:52:13.70 Sensor limits: Intake max 114.7psia; Outlet max 106.3psia @15:52:13.72 SSV.dial :spit @15:52:15.07 SS.seek 0ml @15:52:17.75 SSV.dial CBV @15:52:19.10 CTV.dial :intake @15:52:19.87 SSV.dial CBV @15:52:19.90 CBV.dial SSV @15:52:20.04 Allowing 40s for 7.0ml of remaining 7.0ml @15:52:20.06 Intake.select :open @15:52:22.23 Intake @14.4psia, vacuum from 8.9 to 11.9psi @15:52:22.25 SS.reconfigure :inhaling 2.20ml/s @15:52:22.30 SS.seek 7ml @15:52:28.71 Stroke completed -- waiting for pressure to equalize ... @15:52:28.72 SS.reconfigure :exhale @15:52:28.77 SS.hold @15:52:38.57 Intake.select :close @15:52:38.72 SSV.dial :exhaust @15:52:39.49 Exhaust.select :open @15:52:39.61 SS.seek 0ml @15:52:43.99 Exhaust.select :close @15:52:44.10 Sampling 1000.0ml @15:52:44.11 Intake.select :close @15:52:44.12 Exhaust.select :close @15:52:44.14 SS.reconfigure :default @15:52:44.18 SS.hold @15:52:44.40 CTV.dial :intake @15:52:44.59 SSV.dial CBV @15:52:44.62 CBV.dial SSV @15:52:45.41 Allowing 130s for 25.0ml of remaining 1000.0ml @15:52:45.42 Intake.select :open @15:52:47.59 Intake @15.2psia, vacuum from 9.7 to 12.7psi @15:52:47.61 SS.reconfigure :inhaling 2.20ml/s @15:52:47.66 SS.seek 25ml @15:52:58.63 Vacuum too high -- waiting up to 40 seconds @15:52:58.65 SS.reconfigure :exhale @15:52:58.70 SS.hold @15:53:05.58 Intake @15.1psia, vacuum from 9.6 to 12.6psi @15:53:05.60 SS.reconfigure :inhaling 1.76ml/s @15:53:05.65 SS.seek 25ml @15:53:10.76 Stroke completed -- waiting for pressure to equalize ... @15:53:10.78 SS.reconfigure :exhale @15:53:10.82 SS.hold @15:53:20.91 Intake.select :close @15:53:21.07 SSV.dial :exhaust @15:53:21.86 Exhaust.select :open @15:53:21.98 SS.seek 0ml @15:53:30.73 Exhaust.select :close @15:53:30.85 SSV.dial CBV @15:53:30.87 CBV.dial SSV @15:53:31.67 Allowing 130s for 25.0ml of remaining 975.0ml @15:53:31.69 Intake.select :open @15:53:33.86 Intake @15.4psia, vacuum from 9.9 to 12.9psi @15:53:33.88 SS.reconfigure :inhaling 1.76ml/s @15:53:33.93 SS.seek 25ml @15:53:50.16 Vacuum too high -- waiting up to 40 seconds @15:53:50.18 SS.reconfigure :exhale @15:53:50.23 SS.hold @15:53:56.35 Intake @14.3psia, vacuum from 8.8 to 11.8psi @15:53:56.37 SS.reconfigure :inhaling 1.41ml/s @15:53:56.42 SS.seek 25ml @15:53:58.09 Stroke completed -- waiting for pressure to equalize ... @15:53:58.10 SS.reconfigure :exhale @15:53:58.15 SS.hold @15:54:06.75 Intake.select :close @15:54:06.91 SSV.dial :exhaust @15:54:08.13 Exhaust.select :open @15:54:08.24 SS.seek 0ml @15:54:17.01 Exhaust.select :close @15:54:17.13 SSV.dial CBV @15:54:17.17 CBV.dial SSV @15:54:17.92 Allowing 130s for 25.0ml of remaining 950.0ml @15:54:17.94 Intake.select :open @15:54:20.11 Intake @15.3psia, vacuum from 9.8 to 12.8psi @15:54:20.12 SS.reconfigure :inhaling 1.41ml/s @15:54:20.17 SS.seek 25ml @15:54:40.98 Stroke completed -- waiting for pressure to equalize ... @15:54:40.99 SS.reconfigure :exhale @15:54:41.04 SS.hold @15:54:53.72 Intake.select :close @15:54:53.88 SSV.dial :exhaust @15:54:55.11 Exhaust.select :open @15:54:55.23 SS.seek 0ml @15:55:04.28 Exhaust.select :close @15:55:04.44 SSV.dial CBV @15:55:04.51 CBV.dial SSV @15:55:05.31 Allowing 130s for 25.0ml of remaining 925.0ml @15:55:05.33 Intake.select :open @15:55:07.49 Intake @15.3psia, vacuum from 9.8 to 12.8psi @15:55:07.51 SS.reconfigure :inhaling 1.41ml/s @15:55:07.56 SS.seek 25ml @15:55:27.89 Stroke completed -- waiting for pressure to equalize ... @15:55:27.90 SS.reconfigure :exhale @15:55:27.95 SS.hold @15:55:39.12 Intake.select :close @15:55:39.27 SSV.dial :exhaust @15:55:40.04 Exhaust.select :open @15:55:40.16 SS.seek 0ml @15:55:49.48 Exhaust.select :close @15:55:49.60 SSV.dial CBV @15:55:49.62 CBV.dial SSV @15:55:50.40 Allowing 130s for 25.0ml of remaining 900.0ml @15:55:50.42 Intake.select :open @15:55:52.59 Intake @15.2psia, vacuum from 9.7 to 12.7psi @15:55:52.61 SS.reconfigure :inhaling 1.41ml/s @15:55:52.66 SS.seek 25ml @15:55:52.79 recoverPRVreagent08 @15:55:52.81 PTV.dial :mixing @15:55:53.57 PBV.dial :waste1 @15:55:53.94 delay 30 seconds @15:56:12.93 Stroke completed -- waiting for pressure to equalize ... @15:56:12.95 SS.reconfigure :exhale @15:56:12.99 SS.hold @15:56:23.96 PBV.dial between PSV and waste1 @15:56:24.35 PSV.dial :air @15:56:24.82 PS.reconfigure :fast @15:56:24.87 PS.seek 2.5ml @15:56:26.10 Intake.select :close @15:56:26.27 SSV.dial :exhaust @15:56:27.03 Exhaust.select :open @15:56:27.15 SS.seek 0ml @15:56:35.81 Exhaust.select :close @15:56:35.92 SSV.dial CBV @15:56:35.94 CBV.dial SSV @15:56:36.73 Allowing 130s for 25.0ml of remaining 875.0ml @15:56:36.75 Intake.select :open @15:56:38.91 Intake @15.3psia, vacuum from 9.8 to 12.8psi @15:56:38.93 SS.reconfigure :inhaling 1.41ml/s @15:56:38.98 SS.seek 25ml @15:56:49.24 PSV.dial :mixloop @15:56:49.71 delay 2 seconds @15:56:51.73 PSV.dial :air @15:56:52.19 delay 2 seconds @15:56:54.21 PSV.dial :mixloop @15:56:54.68 delay 2 seconds @15:56:56.69 PSV.dial :air @15:56:57.31 PS.seek 0ml @15:56:59.60 Vacuum too high -- waiting up to 40 seconds @15:56:59.62 SS.reconfigure :exhale @15:56:59.68 SS.hold @15:57:06.30 Intake @14.4psia, vacuum from 8.9 to 11.9psi @15:57:06.32 SS.reconfigure :inhaling 1.13ml/s @15:57:06.37 SS.seek 25ml @15:57:06.98 Stroke completed -- waiting for pressure to equalize ... @15:57:06.99 SS.reconfigure :exhale @15:57:07.04 SS.hold @15:57:13.56 Intake.select :close @15:57:13.72 SSV.dial :exhaust @15:57:14.50 Exhaust.select :open @15:57:14.62 SS.seek 0ml @15:57:21.08 washCycleDA #1 of 2 @15:57:21.09 PSR.select :air @15:57:21.11 PSV.dial PSR @15:57:22.46 Pulling 0.5ml of PSR.wash @15:57:22.47 PSR.select :wash @15:57:22.59 PS.reconfigure :default @15:57:22.63 PS.seek 0.5ml @15:57:23.71 Exhaust.select :close @15:57:23.83 SSV.dial CBV @15:57:23.87 CBV.dial SSV @15:57:24.62 Allowing 130s for 25.0ml of remaining 850.0ml @15:57:24.63 Intake.select :open @15:57:26.81 Intake @15.3psia, vacuum from 9.8 to 12.8psi @15:57:26.83 SS.reconfigure :inhaling 1.13ml/s @15:57:26.88 SS.seek 25ml @15:57:28.58 delay 5 seconds @15:57:33.60 PSR.select :air @15:57:33.71 PS.seek 2ml @15:57:49.83 PTV.dial :puck @15:57:50.58 PSV.dial PTV @15:57:51.47 PBV.dial :waste1 @15:57:51.99 Stroke completed -- waiting for pressure to equalize ... @15:57:52.00 SS.reconfigure :exhale @15:57:52.05 SS.hold @15:57:52.42 PS.seek 1.5ml @15:57:58.39 delay 3 seconds @15:58:01.41 PBV.dial between PSV and waste1 @15:58:01.79 PS.seek 0ml @15:58:04.85 Intake.select :close @15:58:05.01 SSV.dial :exhaust @15:58:05.80 Exhaust.select :open @15:58:05.91 SS.seek 0ml @15:58:14.96 Exhaust.select :close @15:58:15.08 SSV.dial CBV @15:58:15.11 CBV.dial SSV @15:58:15.97 Allowing 130s for 25.0ml of remaining 825.0ml @15:58:15.98 Intake.select :open @15:58:17.89 delay 2:00 @15:58:18.15 Intake @15.2psia, vacuum from 9.7 to 12.7psi @15:58:18.17 SS.reconfigure :inhaling 1.13ml/s @15:58:18.22 SS.seek 25ml @15:58:42.41 Stroke completed -- waiting for pressure to equalize ... @15:58:42.43 SS.reconfigure :exhale @15:58:42.48 SS.hold @15:58:56.92 Intake.select :close @15:58:57.08 SSV.dial :exhaust @15:58:57.87 Exhaust.select :open @15:58:57.99 SS.seek 0ml @15:59:06.70 Exhaust.select :close @15:59:06.81 SSV.dial CBV @15:59:06.83 CBV.dial SSV @15:59:08.07 Allowing 130s for 25.0ml of remaining 800.0ml @15:59:08.09 Intake.select :open @15:59:10.26 Intake @15.2psia, vacuum from 9.7 to 12.7psi @15:59:10.28 SS.reconfigure :inhaling 1.13ml/s @15:59:10.33 SS.seek 25ml @15:59:33.63 Vacuum too high -- waiting up to 40 seconds @15:59:33.64 SS.reconfigure :exhale @15:59:33.70 SS.hold @15:59:42.40 Intake @14.2psia, vacuum from 8.7 to 11.7psi @15:59:42.42 SS.reconfigure :inhaling 0.91ml/s @15:59:42.47 SS.seek 25ml @15:59:44.65 Stroke completed -- waiting for pressure to equalize ... @15:59:44.66 SS.reconfigure :exhale @15:59:44.71 SS.hold @15:59:52.66 Intake.select :close @15:59:52.83 SSV.dial :exhaust @15:59:53.60 Exhaust.select :open @15:59:53.72 SS.seek 0ml @16:00:02.51 Exhaust.select :close @16:00:02.62 SSV.dial CBV @16:00:02.65 CBV.dial SSV @16:00:03.45 Allowing 130s for 25.0ml of remaining 775.0ml @16:00:03.46 Intake.select :open @16:00:05.62 Intake @15.0psia, vacuum from 9.5 to 12.5psi @16:00:05.64 SS.reconfigure :inhaling 0.91ml/s @16:00:05.69 SS.seek 25ml @16:00:17.92 recoverPSRreagent08 @16:00:17.93 PTV.dial :puck @16:00:18.02 PBV.dial :waste1 @16:00:18.39 delay 30 seconds @16:00:35.65 Stroke completed -- waiting for pressure to equalize ... @16:00:35.66 SS.reconfigure :exhale @16:00:35.71 SS.hold @16:00:48.46 PBV.dial between PSV and waste1 @16:00:48.84 PSR.select :air @16:00:48.86 PSV.dial PSR @16:00:49.61 PS.reconfigure :fast @16:00:49.66 PS.seek 2.5ml @16:00:52.11 Intake.select :close @16:00:52.27 SSV.dial :exhaust @16:00:53.05 Exhaust.select :open @16:00:53.17 SS.seek 0ml @16:01:01.99 Exhaust.select :close @16:01:02.11 SSV.dial CBV @16:01:02.13 CBV.dial SSV @16:01:02.93 Allowing 130s for 25.0ml of remaining 750.0ml @16:01:02.94 Intake.select :open @16:01:05.11 Intake @15.0psia, vacuum from 9.5 to 12.5psi @16:01:05.13 SS.reconfigure :inhaling 0.91ml/s @16:01:05.18 SS.seek 25ml @16:01:14.04 PSV.dial PTV @16:01:14.81 delay 5 seconds @16:01:19.83 PSV.dial PSR @16:01:20.61 delay 5 seconds @16:01:25.63 PSV.dial PTV @16:01:26.52 delay 5 seconds @16:01:31.50 Vacuum too high -- waiting up to 40 seconds @16:01:31.51 SS.reconfigure :exhale @16:01:31.57 SS.hold @16:01:31.62 PSV.dial :air @16:01:32.41 PS.seek 0ml @16:01:40.65 Intake @14.4psia, vacuum from 8.9 to 11.9psi @16:01:40.67 SS.reconfigure :inhaling 0.73ml/s @16:01:40.72 SS.seek 25ml @16:01:45.38 Stroke completed -- waiting for pressure to equalize ... @16:01:45.39 SS.reconfigure :exhale @16:01:45.44 SS.hold @16:01:55.25 Intake.select :close @16:01:55.40 SSV.dial :exhaust @16:01:56.11 washCycleDA #2 of 2 @16:01:56.12 PSR.select :air @16:01:56.14 PSV.dial PSR @16:01:56.21 Exhaust.select :open @16:01:56.32 SS.seek 0ml @16:01:57.48 Pulling 0.5ml of PSR.wash @16:01:57.49 PSR.select :wash @16:01:57.61 PS.reconfigure :default @16:01:57.66 PS.seek 0.5ml @16:02:03.64 delay 5 seconds @16:02:05.33 Exhaust.select :close @16:02:05.45 SSV.dial CBV @16:02:05.48 CBV.dial SSV @16:02:06.27 Allowing 130s for 25.0ml of remaining 725.0ml @16:02:06.29 Intake.select :open @16:02:08.45 Intake @14.9psia, vacuum from 9.4 to 12.4psi @16:02:08.47 SS.reconfigure :inhaling 0.73ml/s @16:02:08.52 SS.seek 25ml @16:02:08.65 PSR.select :air @16:02:08.78 PS.seek 2ml @16:02:24.89 PTV.dial :puck @16:02:24.97 PSV.dial PTV @16:02:25.72 PBV.dial :waste1 @16:02:26.15 PS.seek 1.5ml @16:02:32.14 delay 3 seconds @16:02:35.16 PBV.dial between PSV and waste1 @16:02:35.53 PS.seek 0ml @16:02:44.49 Stroke completed -- waiting for pressure to equalize ... @16:02:44.50 SS.reconfigure :exhale @16:02:44.55 SS.hold @16:02:51.70 delay 2:00 @16:03:05.44 Intake.select :close @16:03:05.59 SSV.dial :exhaust @16:03:06.37 Exhaust.select :open @16:03:06.49 SS.seek 0ml @16:03:15.46 Exhaust.select :close @16:03:15.57 SSV.dial CBV @16:03:15.59 CBV.dial SSV @16:03:16.39 Allowing 130s for 25.0ml of remaining 700.0ml @16:03:16.41 Intake.select :open @16:03:18.57 Intake @14.9psia, vacuum from 9.4 to 12.4psi @16:03:18.59 SS.reconfigure :inhaling 0.73ml/s @16:03:18.64 SS.seek 25ml @16:03:50.28 Vacuum too high -- waiting up to 40 seconds @16:03:50.29 SS.reconfigure :exhale @16:03:50.74 SS.hold @16:04:03.62 Intake @14.7psia, vacuum from 9.2 to 12.2psi @16:04:03.64 SS.reconfigure :inhaling 0.59ml/s @16:04:03.69 SS.seek 25ml @16:04:09.45 Stroke completed -- waiting for pressure to equalize ... @16:04:09.47 SS.reconfigure :exhale @16:04:09.51 SS.hold @16:04:22.36 Intake.select :close @16:04:22.52 SSV.dial :exhaust @16:04:23.30 Exhaust.select :open @16:04:23.42 SS.seek 0ml @16:04:32.44 Exhaust.select :close @16:04:32.55 SSV.dial CBV @16:04:32.57 CBV.dial SSV @16:04:33.38 Allowing 130s for 25.0ml of remaining 675.0ml @16:04:33.39 Intake.select :open @16:04:35.55 Intake @14.9psia, vacuum from 9.4 to 12.4psi @16:04:35.57 SS.reconfigure :inhaling 0.59ml/s @16:04:35.62 SS.seek 25ml @16:04:51.72 recoverPSRreagent08 @16:04:51.73 PTV.dial :puck @16:04:51.81 PBV.dial :waste1 @16:04:52.18 delay 30 seconds @16:05:18.21 Vacuum too high -- waiting up to 40 seconds @16:05:18.23 SS.reconfigure :exhale @16:05:18.28 SS.hold @16:05:22.20 PBV.dial between PSV and waste1 @16:05:22.55 PSR.select :air @16:05:22.57 PSV.dial PSR @16:05:23.35 PS.reconfigure :fast @16:05:23.40 PS.seek 2.5ml @16:05:31.64 Intake @15.0psia, vacuum from 9.5 to 12.5psi @16:05:31.66 SS.reconfigure :inhaling 0.47ml/s @16:05:31.71 SS.seek 25ml @16:05:34.01 Stroke completed -- waiting for pressure to equalize ... @16:05:34.02 SS.reconfigure :exhale @16:05:34.07 SS.hold @16:05:45.94 Intake.select :close @16:05:46.10 SSV.dial :exhaust @16:05:46.88 Exhaust.select :open @16:05:46.99 SS.seek 0ml @16:05:47.66 PSV.dial PTV @16:05:48.43 delay 5 seconds @16:05:53.45 PSV.dial PSR @16:05:54.21 delay 5 seconds @16:05:55.77 Exhaust.select :close @16:05:55.88 SSV.dial CBV @16:05:55.91 CBV.dial SSV @16:05:56.69 Allowing 130s for 25.0ml of remaining 650.0ml @16:05:56.70 Intake.select :open @16:05:58.87 Intake @15.1psia, vacuum from 9.6 to 12.6psi @16:05:58.89 SS.reconfigure :inhaling 0.47ml/s @16:05:58.94 SS.seek 25ml @16:05:59.23 PSV.dial PTV @16:05:59.99 delay 5 seconds @16:06:05.01 PSV.dial :air @16:06:05.90 PS.seek 0ml @16:06:29.57 getPMLReagent08 @16:06:29.58 PRV.dial :air @16:06:30.04 delay 5 seconds @16:06:35.06 PTV.dial :PRVmixing @16:06:36.38 delay 5 seconds @16:06:41.40 PRV.dial :ab2 @16:06:41.87 PTV.dial :PRVmixing @16:06:41.88 PSV.dial :mixloop @16:06:42.34 PS.reconfigure :default @16:06:42.39 PS.seek 0.3ml @16:06:46.23 delay 2 seconds @16:06:48.24 PRV.dial :air @16:06:48.79 PS.seek 0.55ml @16:06:52.17 addPMLReagent08 @16:06:52.18 PSV.dial :air @16:06:52.65 PS.seek 2ml @16:06:54.50 Stroke completed -- waiting for pressure to equalize ... @16:06:54.51 SS.reconfigure :exhale @16:06:55.00 SS.hold @16:07:08.22 PSV.dial :PML @16:07:08.69 PTV.dial :mixing @16:07:09.46 PBV.dial :waste1 @16:07:09.91 PS.seek 1.85ml @16:07:12.35 PBV.dial between PSV and waste1 @16:07:12.73 PS.seek 0ml @16:07:19.53 Intake.select :close @16:07:19.70 SSV.dial :exhaust @16:07:20.49 Exhaust.select :open @16:07:20.61 SS.seek 0ml @16:07:29.42 Exhaust.select :close @16:07:29.54 SSV.dial CBV @16:07:29.57 CBV.dial SSV @16:07:30.34 Allowing 130s for 25.0ml of remaining 625.0ml @16:07:30.36 Intake.select :open @16:07:32.53 delay 2 seconds @16:07:32.58 Intake @15.0psia, vacuum from 9.5 to 12.5psi @16:07:32.60 SS.reconfigure :inhaling 0.47ml/s @16:07:32.65 SS.seek 25ml @16:07:34.56 delay 8:00 @16:08:25.58 Vacuum too high -- waiting up to 40 seconds @16:08:25.60 SS.reconfigure :exhale @16:08:25.65 SS.hold @16:08:44.22 Intake @15.4psia, vacuum from 9.9 to 12.9psi @16:08:44.24 SS.reconfigure :inhaling 0.38ml/s @16:08:44.29 SS.seek 25ml @16:08:47.71 Stroke completed -- waiting for pressure to equalize ... @16:08:47.73 SS.reconfigure :exhale @16:08:47.77 SS.hold @16:09:04.61 Intake.select :close @16:09:04.84 SSV.dial :exhaust @16:09:05.62 Exhaust.select :open @16:09:05.74 SS.seek 0ml @16:09:14.87 Exhaust.select :close @16:09:14.99 SSV.dial CBV @16:09:15.02 CBV.dial SSV @16:09:15.83 Allowing 130s for 25.0ml of remaining 600.0ml @16:09:15.84 Intake.select :open @16:09:18.00 Intake @14.9psia, vacuum from 9.4 to 12.4psi @16:09:18.02 SS.reconfigure :inhaling 0.38ml/s @16:09:18.07 SS.seek 25ml @16:10:26.29 Stroke completed -- waiting for pressure to equalize ... @16:10:26.30 SS.reconfigure :exhale @16:10:26.35 SS.hold @16:10:59.10 Intake.select :close @16:10:59.26 SSV.dial :exhaust @16:11:00.04 Exhaust.select :open @16:11:00.16 SS.seek 0ml @16:11:08.96 Exhaust.select :close @16:11:09.08 SSV.dial CBV @16:11:09.11 CBV.dial SSV @16:11:09.92 Allowing 130s for 25.0ml of remaining 575.0ml @16:11:09.93 Intake.select :open @16:11:12.09 Intake @14.9psia, vacuum from 9.4 to 12.4psi @16:11:12.11 SS.reconfigure :inhaling 0.38ml/s @16:11:12.16 SS.seek 25ml @16:12:14.55 Vacuum too high -- waiting up to 40 seconds @16:12:14.56 SS.reconfigure :exhale @16:12:14.62 SS.hold @16:12:35.65 Intake @15.2psia, vacuum from 9.7 to 12.7psi @16:12:35.67 SS.reconfigure :inhaling 0.31ml/s @16:12:35.72 SS.seek 25ml @16:12:44.18 Stroke completed -- waiting for pressure to equalize ... @16:12:44.19 SS.reconfigure :exhale @16:12:44.24 SS.hold @16:13:06.72 Intake.select :close @16:13:06.88 SSV.dial :exhaust @16:13:07.66 Exhaust.select :open @16:13:07.78 SS.seek 0ml @16:13:16.60 Exhaust.select :close @16:13:16.72 SSV.dial CBV @16:13:16.74 CBV.dial SSV @16:13:17.54 Allowing 130s for 25.0ml of remaining 550.0ml @16:13:17.55 Intake.select :open @16:13:19.72 Intake @14.8psia, vacuum from 9.3 to 12.3psi @16:13:19.74 SS.reconfigure :inhaling 0.31ml/s @16:13:19.79 SS.seek 25ml @16:14:48.11 Stroke completed -- waiting for pressure to equalize ... @16:14:48.13 SS.reconfigure :exhale @16:14:48.17 SS.hold @16:15:27.56 SS.reconfigure :default @16:15:27.61 SS.hold @16:15:27.68 Intake.select :close @16:15:27.84 ShallowSampler::Clogged in WCR -- Filtering too Slowly after 475.0ml @16:15:28.27 SSV.dial :exhaust @16:15:29.10 Exhaust.select :open @16:15:29.22 SS.reconfigure :exhale @16:15:29.27 SS.seek 0ml @16:15:34.58 recoverPRVreagent08 @16:15:34.60 PTV.dial :mixing @16:15:34.67 PBV.dial :waste1 @16:15:35.05 delay 30 seconds @16:15:38.35 Exhaust.select :close @16:15:38.46 Sampled 475.0ml @16:15:38.48 Drying puck before fixing for 5:00 @16:15:38.49 CBV.dial :waste2b @16:15:39.25 delay 1:00 @16:16:05.07 PBV.dial between PSV and waste1 @16:16:05.45 PSV.dial :air @16:16:05.92 PS.reconfigure :fast @16:16:05.97 PS.seek 2.5ml @16:16:30.34 PSV.dial :mixloop @16:16:30.81 delay 2 seconds @16:16:32.83 PSV.dial :air @16:16:33.29 delay 2 seconds @16:16:35.31 PSV.dial :mixloop @16:16:35.78 delay 2 seconds @16:16:37.80 PSV.dial :air @16:16:38.27 PS.seek 0ml @16:16:39.27 CSV.dial :air @16:16:39.72 CS.reconfigure :fast @16:16:39.78 CS.seek 10ml @16:17:02.22 washCycleDA #1 of 3 @16:17:02.23 PSR.select :air @16:17:02.25 PSV.dial PSR @16:17:03.59 Pulling 0.5ml of PSR.wash @16:17:03.60 PSR.select :wash @16:17:03.72 PS.reconfigure :default @16:17:03.77 PS.seek 0.5ml @16:17:04.44 Pumping wcr puck from tube 2 up to 35psi... @16:17:04.46 CBV.dial SSV @16:17:05.74 CBV.dial between CSV and waste2b @16:17:06.71 CSV.dial between air and CTV @16:17:07.04 CS.seek 7.328ml @16:17:09.65 delay 5 seconds @16:17:13.97 CTV.dial CSV,avoiding: :air @16:17:14.00 CSV.dial CTV @16:17:14.67 PSR.select :air @16:17:14.79 PS.seek 2ml @16:17:14.86 CS.reconfigure :pressurize @16:17:14.92 CS.seek 0ml @16:17:28.99 CBV.dial :waste2b @16:17:29.35 CS.reconfigure :default @16:17:29.40 CS.seek 0ml @16:17:31.47 PTV.dial :puck @16:17:32.23 PSV.dial PTV @16:17:32.98 PBV.dial :waste1 @16:17:33.42 PS.seek 1.5ml @16:17:35.28 delay 1:00 @16:17:39.44 delay 3 seconds @16:17:42.46 PBV.dial between PSV and waste1 @16:17:42.83 PS.seek 0ml @16:17:59.03 delay 2:00 @16:18:35.30 CTV.dial :air,avoiding: :intake @16:18:36.66 Fix #1 of 2 (with RNAlater) for 2:30 @16:18:36.67 CSV.dial CSR @16:18:37.12 Pulling 2ml of CSR.RNAlater @16:18:37.13 CSR.select :RNAlater @16:18:37.25 CS.seek 2ml @16:18:43.18 delay 5 seconds @16:18:48.20 CSR.select :air @16:18:48.33 CS.reconfigure :fast @16:18:48.38 CS.seek 10ml @16:19:08.43 Pumping wcr puck from tube 2 up to 35psi... @16:19:08.45 CBV.dial SSV @16:19:09.71 CBV.dial between CSV and waste2b @16:19:10.68 CSV.dial between air and CTV @16:19:11.29 CS.seek 7.259ml @16:19:18.47 CTV.dial CSV,avoiding: :air @16:19:18.50 CSV.dial CTV @16:19:19.89 CS.reconfigure :pressurize @16:19:19.95 CS.seek 0ml @16:19:34.21 delay 2:30 @16:19:59.05 recoverPSRreagent08 @16:19:59.06 PTV.dial :puck @16:19:59.24 PBV.dial :waste1 @16:19:59.60 delay 30 seconds @16:20:29.63 PBV.dial between PSV and waste1 @16:20:29.99 PSR.select :air @16:20:30.01 PSV.dial PSR @16:20:30.79 PS.reconfigure :fast @16:20:30.84 PS.seek 2.5ml @16:20:55.11 PSV.dial PTV @16:20:55.88 delay 5 seconds @16:21:00.90 PSV.dial PSR @16:21:01.66 delay 5 seconds @16:21:06.68 PSV.dial PTV @16:21:07.45 delay 5 seconds @16:21:12.47 PSV.dial :air @16:21:13.23 PS.seek 0ml @16:21:36.96 washCycleDA #2 of 3 @16:21:36.97 PSR.select :air @16:21:36.99 PSV.dial PSR @16:21:38.32 Pulling 0.5ml of PSR.wash @16:21:38.79 PSR.select :wash @16:21:38.92 PS.reconfigure :default @16:21:38.97 PS.seek 0.5ml @16:21:44.96 delay 5 seconds @16:21:49.98 PSR.select :air @16:21:50.10 PS.seek 2ml @16:22:04.22 CBV.dial :waste2b @16:22:04.57 CS.reconfigure :default @16:22:04.63 CS.seek 0ml @16:22:06.24 PTV.dial :puck @16:22:06.32 PSV.dial PTV @16:22:07.07 PBV.dial :waste1 @16:22:07.49 PS.seek 1.5ml @16:22:10.15 delay 1:00 @16:22:13.43 delay 3 seconds @16:22:16.45 PBV.dial between PSV and waste1 @16:22:16.81 PS.seek 0ml @16:22:32.99 delay 2:00 @16:23:10.18 CTV.dial :air,avoiding: :intake @16:23:11.51 Fix #2 of 2 (with RNAlater) for 2:30 @16:23:11.53 CSV.dial CSR @16:23:11.96 Pulling 2ml of CSR.RNAlater @16:23:11.97 CSR.select :RNAlater @16:23:12.09 CS.seek 2ml @16:23:18.27 delay 5 seconds @16:23:23.29 CSR.select :air @16:23:23.41 CS.reconfigure :fast @16:23:23.47 CS.seek 10ml @16:23:43.31 Pumping wcr puck from tube 2 up to 35psi... @16:23:43.32 CBV.dial SSV @16:23:44.58 CBV.dial between CSV and waste2b @16:23:45.53 CSV.dial between air and CTV @16:23:46.14 CS.seek 7.242ml @16:23:53.25 CTV.dial CSV,avoiding: :air @16:23:53.28 CSV.dial CTV @16:23:54.67 CS.reconfigure :pressurize @16:23:54.72 CS.seek 0ml @16:24:13.74 CSV.dial :air @16:24:14.21 CS.reconfigure :fast @16:24:14.27 CS.seek 10ml @16:24:33.01 recoverPSRreagent08 @16:24:33.03 PTV.dial :puck @16:24:33.10 PBV.dial :waste1 @16:24:33.47 delay 30 seconds @16:24:39.34 CSV.dial between air and CTV @16:24:39.69 CS.seek 3.71ml @16:24:54.81 CTV.dial CSV,avoiding: :air @16:24:54.84 CSV.dial CTV @16:24:55.20 CS.reconfigure :pressurize @16:24:55.25 CS.seek 0ml @16:25:02.11 delay 2:30 @16:25:03.49 PBV.dial between PSV and waste1 @16:25:03.86 PSR.select :air @16:25:03.88 PSV.dial PSR @16:25:04.65 PS.reconfigure :fast @16:25:04.70 PS.seek 2.5ml @16:25:28.92 PSV.dial PTV @16:25:29.69 delay 5 seconds @16:25:34.71 PSV.dial PSR @16:25:35.46 delay 5 seconds @16:25:40.48 PSV.dial PTV @16:25:41.24 delay 5 seconds @16:25:46.26 PSV.dial :air @16:25:47.02 PS.seek 0ml @16:26:10.58 washCycleDA #3 of 3 @16:26:10.59 PSR.select :air @16:26:10.61 PSV.dial PSR @16:26:11.94 Pulling 0.5ml of PSR.wash @16:26:11.95 PSR.select :wash @16:26:12.07 PS.reconfigure :default @16:26:12.12 PS.seek 0.5ml @16:26:18.08 delay 5 seconds @16:26:23.10 PSR.select :air @16:26:23.22 PS.seek 2ml @16:26:39.42 PTV.dial :puck @16:26:39.50 PSV.dial PTV @16:26:40.37 PBV.dial :waste1 @16:26:40.81 PS.seek 1.5ml @16:26:46.73 delay 3 seconds @16:26:49.75 PBV.dial between PSV and waste1 @16:26:50.13 PS.seek 0ml @16:27:06.33 delay 2:00 @16:27:32.13 CBV.dial :waste2b @16:27:32.46 CS.reconfigure :default @16:27:32.51 CS.seek 0ml @16:27:36.33 delay 1:00 @16:28:36.36 CTV.dial :air,avoiding: :intake @16:29:06.36 recoverPSRreagent08 @16:29:06.37 PTV.dial :puck @16:29:06.43 PBV.dial :waste1 @16:29:06.81 delay 30 seconds @16:29:36.84 PBV.dial between PSV and waste1 @16:29:37.21 PSR.select :air @16:29:37.23 PSV.dial PSR @16:29:37.99 PS.reconfigure :fast @16:29:38.03 PS.seek 2.5ml @16:30:02.55 PSV.dial PTV @16:30:03.37 delay 5 seconds @16:30:08.39 PSV.dial PSR @16:30:09.15 delay 5 seconds @16:30:14.17 PSV.dial PTV @16:30:14.93 delay 5 seconds @16:30:19.96 PSV.dial :air @16:30:20.74 PS.seek 0ml @16:30:44.29 Gate.power :camera,:ON @16:30:44.31 Mixing DA Substrates @16:30:44.32 PSR.select :air @16:30:44.33 PSV.dial PSR @16:30:45.77 Pulling 0.25ml of PSR.sub1 @16:30:45.79 PSR.select :sub1 @16:30:46.01 PS.reconfigure :default @16:30:46.13 PS.seek 0.25ml @16:30:49.52 delay 5 seconds @16:30:54.54 Pulling 0.25ml of PSR.sub2 @16:30:54.56 PSR.select :sub2 @16:30:54.68 PS.seek 0.5ml @16:30:58.10 delay 5 seconds @16:31:03.12 PSR.select :air @16:31:03.24 PS.seek 2ml @16:31:19.29 PTV.dial :puck @16:31:19.37 PSV.dial PTV @16:31:20.15 PBV.dial :waste1 @16:31:20.59 PS.seek 1.5ml @16:31:26.54 delay 3 seconds @16:31:29.56 PBV.dial between PSV and waste1 @16:31:29.94 PS.seek 0ml @16:31:46.13 delay 10 seconds @16:31:56.16 recoverPSRreagent08 @16:31:56.17 PTV.dial :puck @16:31:56.24 PBV.dial :waste1 @16:31:56.62 delay 30 seconds @16:32:26.65 PBV.dial between PSV and waste1 @16:32:27.02 PSR.select :air @16:32:27.04 PSV.dial PSR @16:32:27.84 PS.reconfigure :fast @16:32:27.89 PS.seek 2.5ml @16:32:52.44 PSV.dial PTV @16:32:53.35 delay 5 seconds @16:32:58.37 PSV.dial PSR @16:32:59.12 delay 5 seconds @16:33:04.14 PSV.dial PTV @16:33:04.92 delay 5 seconds @16:33:09.94 PSV.dial :air @16:33:10.84 PS.seek 0ml @16:33:35.20 Additional air clear @16:33:35.21 PSR.select :air @16:33:35.23 PSV.dial PSR @16:33:36.59 Pulling 2ml of PSR.air @16:33:36.60 PSR.select :air @16:33:36.62 PS.reconfigure :default @16:33:36.67 PS.seek 2ml @16:33:57.85 delay 5 seconds @16:34:02.87 PTV.dial :puck @16:34:02.96 PSV.dial PTV @16:34:03.87 PBV.dial :waste1 @16:34:04.30 PS.seek 1.5ml @16:34:10.32 delay 3 seconds @16:34:13.34 PBV.dial between PSV and waste1 @16:34:13.71 delay 1 second @16:34:14.73 PSV.dial :waste1 @16:34:15.82 PS.seek 0ml @16:34:32.54 Processing Puck Heater at 28C before imaging @16:34:32.55 PH.coast @16:34:32.67 Imaging da puck in Processing Clamp while storing it to tube 1 @16:34:32.86 SC.reconfigure :tight @16:34:32.91 SC.seek 1 @16:34:36.54 SC.configure :limp @16:34:36.76 Elbow.reconfigure :tight @16:34:36.81 Elbow.seek 1 @16:34:38.59 Forearm.reconfigure :tight @16:34:38.64 Forearm.seek 1 + 450 counts @16:34:39.69 SE.reconfigure :pick @16:34:39.75 SE.seek :beamBreak @16:34:41.30 SE.configure :default @16:34:41.46 Elbow.reconfigure :limp @16:34:41.51 Forearm.reconfigure :loose @16:34:41.56 Forearm.seek 1 @16:34:41.85 Elbow.hold @16:34:41.91 Elbow.reconfigure :tight @16:34:42.01 SE.seek 25.03 Pucks below Top Plate @16:34:42.47 Forearm.force -20 @16:34:42.51 Forearm.seek 1 + 318 counts @16:34:42.81 Forearm.reconfigure :tight @16:34:42.86 Forearm.seek 1 + 450 counts @16:34:43.07 SE.seek 26.03 Pucks below Top Plate @16:34:43.66 Forearm.reconfigure :loose @16:34:43.71 Forearm.seek :retract @16:34:44.65 Elbow.seek PC @16:34:47.14 Elbow.reconfigure :loose @16:34:47.24 Forearm.seek PC @16:34:48.20 Hand.close @16:34:48.78 PC.seek :open @16:34:53.98 Forearm.seek :retract @16:34:55.01 Elbow.reconfigure :tight @16:34:55.06 Elbow.seek 1 @16:34:57.78 Forearm.reconfigure :tight @16:34:57.83 Forearm.seek 1 + 450 counts @16:34:58.80 Hand.open @16:34:59.46 SE.seek 25.68 Pucks below Top Plate @16:34:59.72 SE.reconfigure :seal @16:34:59.77 SE.seek 24.72 Pucks below Top Plate @16:35:00.30 Lights, camera, ACTION! @16:35:00.33 PuckCamera: snap -bin=2,2 -auto /var/log/esp/da23oct0916h600ml.tif @16:35:19.83 Waiting >20 seconds for da to relinquish FlushPuck @16:35:23.78 SX HX9/16 (USB:07): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 20.059 seconds /var/log/esp/da23oct0916h600ml.tif: TIFF Upload Complete @16:35:23.80 PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da23oct0916h600ml15s.tif @16:35:39.44 SX HX9/16 (USB:07): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15 seconds /var/log/esp/midres/da23oct0916h600ml15s.tif: TIFF Upload Complete @16:35:39.46 PuckCamera: snap -bin=2,2 20.0 /var/log/esp/midres/da23oct0916h600ml20s.tif @16:36:00.11 SX HX9/16 (USB:07): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 20 seconds /var/log/esp/midres/da23oct0916h600ml20s.tif: TIFF Upload Complete @16:36:00.13 Gate.power :camera,:OFF @16:36:00.15 DAfans.email "20.059 sec auto-exposure",Subject: "da23oct0916h600ml images!" @16:36:00.59 Elbow.seek 1 @16:36:01.09 Forearm.seek 1 + 450 counts @16:36:01.18 SE.reconfigure :gently @16:36:01.23 SE.seek 25.43 Pucks below Top Plate @16:36:01.91 Elbow.reconfigure :limp @16:36:01.97 Forearm.reconfigure :loose @16:36:02.03 Forearm.seek 1 @16:36:02.35 Elbow.hold @16:36:02.42 Elbow.reconfigure :tight @16:36:02.47 SE.reconfigure :default @16:36:02.52 SE.seek 25.62 Pucks below Top Plate @16:36:02.95 Forearm.force -20 @16:36:03.00 Forearm.seek 1 + 307 counts @16:36:03.41 Forearm.reconfigure :tight @16:36:03.46 Forearm.seek 1 + 450 counts @16:36:03.89 SE.seek :down @16:36:04.86 SE.stop @16:36:05.55 Forearm.reconfigure :loose @16:36:05.61 Forearm.seek :retract @16:36:06.64 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @16:36:06.93 Elbow.seek FlushPuck::Garage @16:36:08.85 Elbow.reconfigure :loose @16:36:09.07 Forearm.seek :garage @16:36:10.13 Hand.close @16:36:11.05 Forearm.seek :retract @16:36:12.04 Elbow.reconfigure :tight @16:36:12.16 Elbow.seek PC @16:36:13.56 Elbow.reconfigure :loose @16:36:13.78 Forearm.reconfigure :tight @16:36:13.87 Forearm.seek PC @16:36:15.46 PC.seek :closed @16:36:20.39 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.63 @16:36:20.53 Hand.open @16:36:21.30 Forearm.reconfigure :loose @16:36:21.40 Forearm.seek :retract @16:36:22.44 PSR.select :air @16:36:22.50 Storing wcr puck in Collection Clamp to tube 1 @16:36:22.55 PSV.dial PSR @16:36:23.30 Pulling 0.5ml of PSR.flush @16:36:23.38 PSR.select :flush @16:36:24.76 PS.seek 0.5ml @16:36:24.90 Elbow.reconfigure :tight @16:36:25.00 Elbow.seek 1 @16:36:27.77 Forearm.reconfigure :tight @16:36:27.89 Forearm.seek 1 + 450 counts @16:36:29.03 SE.reconfigure :pick @16:36:29.15 SE.seek :beamBreak @16:36:30.68 SE.configure :default @16:36:30.80 delay 5 seconds @16:36:31.10 Elbow.reconfigure :limp @16:36:31.20 Forearm.reconfigure :loose @16:36:31.30 Forearm.seek 1 @16:36:31.63 Elbow.hold @16:36:31.80 Elbow.reconfigure :tight @16:36:32.06 SE.seek 26.04 Pucks below Top Plate @16:36:32.71 Forearm.force -20 @16:36:32.79 Forearm.seek 1 + 319 counts @16:36:33.44 Forearm.reconfigure :tight @16:36:33.58 Forearm.seek 1 + 450 counts @16:36:33.94 SE.seek 27.04 Pucks below Top Plate @16:36:34.79 Forearm.reconfigure :loose @16:36:34.92 Forearm.seek :retract @16:36:35.86 PSR.select :air @16:36:35.96 Elbow.seek CC @16:36:36.21 PS.seek 2.5ml @16:36:37.83 Elbow.reconfigure :loose @16:36:38.04 Forearm.seek CC @16:36:39.08 Hand.close @16:36:40.28 upsync @16:36:40.31 CC.seek :open @16:36:45.10 Forearm.seek :retract @16:36:46.10 Elbow.reconfigure :tight @16:36:46.15 Elbow.seek 1 @16:36:47.95 Elbow.reconfigure :limp @16:36:48.00 Forearm.seek 1 @16:36:48.99 Elbow.hold @16:36:49.06 Elbow.reconfigure :tight @16:36:49.11 Hand.open @16:36:49.90 Forearm.force -20 @16:36:49.95 Forearm.seek 1 + 319 counts @16:36:50.27 Forearm.reconfigure :tight @16:36:50.33 Forearm.seek 1 + 450 counts @16:36:50.61 SE.seek :down @16:36:51.56 SE.stop @16:36:51.75 Forearm.reconfigure :loose @16:36:51.80 Forearm.seek :retract @16:36:52.80 Starting finalPurge @16:36:57.47 delay 1 second @16:36:58.49 PRV.dial :waste2,avoiding: :ab2 @16:37:00.35 PTV.dial :PRVmixing @16:37:01.66 PSV.dial :mixloop @16:37:02.70 PS.seek :empty @16:37:12.01
Waiting >20 seconds for psFlush to relinquish FlushPuck @16:37:29.13 PSR.select :air @16:37:29.15 PSV.dial PSR @16:37:30.19 Pulling 0.5ml of PSR.flush @16:37:30.20 PSR.select :flush @16:37:30.32 PS.seek 0.5ml @16:37:36.25 delay 5 seconds @16:37:41.27 PSR.select :air @16:37:41.39 PS.seek 2.5ml @16:38:02.69 delay 1 second @16:38:04.09 PBV.dial :waste1 @16:38:04.52 PTV.dial :mixing @16:38:05.27 PSV.dial :mixloop @16:38:06.32 PS.seek :empty @16:38:32.69 PSV.dial PSR @16:38:33.74 PSR.select :flush @16:38:33.86 PS.seek 1ml @16:38:44.97 PSR.select :air @16:38:45.09 PS.seek 2.5ml @16:39:01.19 PSV.dial PTV @16:39:02.06 PTV.dial :puck @16:39:02.81 PBV.dial :waste1 @16:39:02.90 PS.seek 0ml @16:39:29.19 PSV.dial PSR @16:39:30.05 PSR.select :flush @16:39:30.18 PS.seek 0.5ml @16:39:36.09 PSR.select :air @16:39:36.21 PS.seek 2.5ml @16:39:57.47 PSV.dial PTV @16:39:58.20 PBV.dial PSV @16:39:58.77 PS.seek 0ml @16:40:25.15 PSV.dial PBV @16:40:25.63 PS.seek 2.5ml @16:40:52.03 PSV.dial :waste1 @16:40:52.77 PS.seek 0ml @16:41:19.15 PSV.dial PSR @16:41:19.62 PSR.select :air @16:41:19.65 PS.seek 2.5ml @16:41:46.06 PSV.dial PTV @16:41:46.92 PBV.dial PSV @16:41:47.00 PS.seek 0ml @16:42:13.37 PSV.dial PBV @16:42:13.83 PS.seek 2.5ml @16:42:40.15 PSV.dial :waste1 @16:42:40.89 PS.seek 0ml @16:43:07.31 PSV.dial PSR @16:43:07.78 PSR.select :flush @16:43:07.90 PS.seek 1ml @16:43:18.99 PSR.select :air @16:43:19.11 PS.seek 2.5ml @16:43:35.31 PSV.dial PTV @16:43:36.17 PTV.dial :puck @16:43:36.25 PBV.dial :waste1 @16:43:37.26 PS.seek 0ml @16:44:03.68 PSV.dial PSR @16:44:04.43 PSR.select :flush @16:44:04.56 PS.seek 0.5ml @16:44:10.56 PSR.select :air @16:44:10.68 PS.seek 2.5ml @16:44:31.96 PSV.dial PTV @16:44:32.73 PBV.dial PSV @16:44:33.30 PS.seek 0ml @16:44:59.68 PSV.dial PBV @16:45:00.16 PS.seek 2.5ml @16:45:26.56 PSV.dial :waste1 @16:45:27.30 PS.seek 0ml @16:45:53.68 PSV.dial PSR @16:45:54.15 PSR.select :air @16:45:54.18 PS.seek 2.5ml @16:46:20.52 PSV.dial PTV @16:46:21.26 PBV.dial PSV @16:46:21.35 PS.seek 0ml @16:46:47.74 PSV.dial PBV @16:46:48.22 PS.seek 2.5ml @16:47:14.58 PSV.dial :waste1 @16:47:15.33 PS.seek 0ml @16:47:41.75
Loading FlushPuck into Collection Clamp from Processing Clamp @16:47:41.94 Elbow.seek PC @16:47:44.43 Elbow.reconfigure :loose @16:47:44.53 Forearm.seek PC @16:47:45.52 Hand.close @16:47:46.14 PC.seek :open @16:47:50.89 Forearm.seek :retract @16:47:51.89 Elbow.reconfigure :tight @16:47:51.94 Elbow.seek CC @16:47:55.51 Elbow.reconfigure :loose @16:47:55.61 Forearm.reconfigure :tight @16:47:55.66 Forearm.seek CC @16:47:57.33 CC.seek :closed @16:48:01.85 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.35 @16:48:01.92 Hand.open @16:48:02.57 Forearm.reconfigure :loose @16:48:02.62 Forearm.seek :retract @16:48:03.60 Flushing the Collection Valve::Manifold @16:48:03.73 CSV.dial CSR @16:48:04.17 Pulling 5ml of CSR.flush @16:48:04.18 CSR.select :flush @16:48:04.30 CS.seek 5ml @16:48:17.87 delay 5 seconds @16:48:22.89 CSR.select :air @16:48:23.06 CS.reconfigure :fast @16:48:23.11 CS.seek 7.501ml @16:48:29.90 CSV.dial :waste @16:48:31.20 CS.reconfigure :default @16:48:31.25 CS.seek 0ml @16:48:51.22 Flushing lower manifold's waste1 port @16:48:51.34 CSV.dial :flush @16:48:51.78 CS.reconfigure :fast @16:48:51.83 CS.seek 5ml @16:49:04.53 delay 5 seconds @16:49:09.55 CSV.dial :air @16:49:10.08 CS.seek 7ml @16:49:15.69 CSV.dial CTV @16:49:15.71
CTV.dial CSV @16:49:17.09 CBV.dial :waste1 @16:49:17.86 CS.seek 0ml @16:49:34.50 Flushing lower manifold's waste2 port @16:49:34.62 CSV.dial :flush @16:49:35.36 CS.seek 5ml @16:49:48.03 delay 5 seconds @16:49:53.05 CSV.dial :air @16:49:53.58 CS.seek 7ml @16:49:59.15 CSV.dial CTV @16:49:59.18
CTV.dial CSV @16:49:59.65 CBV.dial :waste2 @16:50:01.16 CS.seek 0ml @16:50:17.84 Flushing lower manifold's waste2b port @16:50:17.97 CSV.dial :flush @16:50:18.83 CS.seek 5ml @16:50:31.44 delay 5 seconds @16:50:36.46 CSV.dial :air @16:50:36.97 CS.seek 7ml @16:50:42.56 CSV.dial CTV @16:50:42.59
CTV.dial CSV @16:50:43.03 CBV.dial :waste2b @16:50:44.08 CS.seek 0ml @16:51:00.78 Disinfecting external Intake with kill @16:51:00.90 CSV.dial CSR @16:51:01.34 Pulling 8ml of CSR.kill @16:51:01.35 CSR.select :kill @16:51:01.47 CS.seek 8ml @16:51:21.34 delay 5 seconds @16:51:26.36 CSR.select :air @16:51:26.48 CS.seek 10ml @16:51:31.97 CSV.dial CBV @16:51:32.00
CBV.dial CSV @16:51:33.07 CTV.dial :intake @16:51:33.84 Intake.select :open @16:51:33.95 CS.seek 2ml @16:51:52.71 Intake.select :close @16:51:52.81 CTV.dial :air @16:51:53.56 CS.reconfigure :default @16:51:53.61 CS.seek 0ml @16:51:59.59 CSV.dial CSR @16:52:00.61 Pulling 5ml of CSR.flush CSR.select :flush @16:52:00.73 CS.seek 5ml @16:52:14.53 delay 5 seconds @16:52:19.54 CSR.select :air @16:52:19.66 CS.seek 10ml @16:52:33.24 CSV.dial CBV @16:52:34.26 CS.seek 0ml @16:53:00.65 Garaging FlushPuck in Collection Clamp @16:53:00.72 Elbow.reconfigure :tight @16:53:00.77 Elbow.seek CC @16:53:01.04 Elbow.reconfigure :loose @16:53:01.15 Forearm.seek CC @16:53:02.19 Hand.close @16:53:02.76 CC.seek :open @16:53:07.39 Forearm.seek :retract @16:53:08.86 Elbow.reconfigure :tight @16:53:08.93 Elbow.seek :garage @16:53:11.87 Elbow.reconfigure :loose @16:53:11.98 Forearm.reconfigure :tight @16:53:12.03 Forearm.seek :garage @16:53:13.10 Hand.open @16:53:13.82 Forearm.reconfigure :loose @16:53:13.87 Forearm.seek :retract @16:53:14.85 Completed [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] begun 14:31:26 @16:53:14.90 Asleep after completing DA_WCR_100923 assays @16:53:14.92 GoodNews.email "{1 => 28,\n 2 => 5,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@16:50:31, 26% flow, 18.1C, 16% humidity, 14.6psia, 13.661V, 0.466A, 121.428Ah, 6.36W\nSampled 475.0ml",Subject: "Asleep after completing DA_WCR_100923 assays" @16:53:20.50 upsync + ESPwhich DA_WCR_101223.rb + fn=DA_WCR_101223.rb + IFS=: + set /home/esp/esp2/mission . + fullPath=/home/esp/esp2/mission/DA_WCR_101223.rb + [ -f /home/esp/esp2/mission/DA_WCR_101223.rb ] + realpath /home/esp/esp2/mission/DA_WCR_101223.rb + return 0 + scriptPath=/home/esp/esp2local/mission/DA_WCR_101223.rb + exec ruby /home/esp/esp2/lib/esp.rb /home/esp/esp2local/mission/DA_WCR_101223.rb @14:49:52.36UTC12-Oct-23
ESP2 v3.42 I2C Gateway v3.20 for core bus @14:49:55.01 Configuration for short puck jake 1/11/21 by brent@mbari.org /home/esp/esp2/type/shallow/jake/configure.rb @14:49:58.68 Polling Can every 10:00 @14:49:58.72 Cleanly exited at 14:45:22UTC12-Oct-23 @14:49:58.76 Loading /home/esp/esp2local/mission/DA_WCR_101223.rb mission @14:49:58.82 Preparing to sleep until + 00:00:05 @14:49:58.83 GoodNews.email "Starting DA_WCR_101223 w/pucks in tube 2\n{1 => 28,\n 2 => 5,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@14:49:58, 27% flow, 17.6C, 17% humidity, 14.6psia, 13.730V, 0.248A, 137.995Ah, 3.41W",Subject: "DA_WCR_101223 begun" @14:49:58.89
Asleep til + 00:00:05 @14:49:58.93 Continuing at 14:50 @14:49:59.16 Gate.power :core,:OFF @14:49:59.19 Gate.power :raw,:OFF @14:49:59.20 Shutdown @14:50:01.71 GoodNews.email "Then, in tube 2: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_101223.rb:14\n{1 => 28,\n 2 => 5,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@14:49:58, 27% flow, 17.6C, 17% humidity, 14.6psia, 13.730V, 0.254A, 137.995Ah, 3.49W",Subject: "Asleep until 14:50" @14:50:03.17 upsync @14:50:03.80
Powering core bus @14:50:06.07 upsync @14:50:09.52
Gate.power :raw,:ON @14:50:10.38 CS.configure :default @14:50:10.43 PS.configure :default @14:50:10.48 SS.configure :default @14:50:10.52 CC.configure :default @14:50:10.57 PC.configure :default @14:50:10.64 Forearm.configure :loose @14:50:10.69 Elbow.configure :loose @14:50:10.77 SC.configure :loose @14:50:10.82 SE.configure :default @14:50:10.86 CH.configure :default @14:50:10.90 PH.configure :default @14:50:10.94 All dwarf controllers running firmware version 3.85 @14:50:11.05 Valve.reset @14:50:11.28 Intake.select :close @14:50:11.32 Exhaust.select :close @14:50:11.35 CTV.dial :air @14:50:11.47 PSV.dial :air @14:50:11.57 CSV.dial :waste1 @14:50:11.61
Forearm.home.seek :retract @14:50:12.05 SSV.dial :spit,avoiding: :exhaust @14:50:12.42 Hand.close @14:50:12.45
Elbow.reconfigure :loose @14:50:12.54 Elbow.jog 1500 @14:50:12.63 PS.home.seek 0ml @14:50:12.92 CS.reconfigure :fast @14:50:12.96 SS.home.seek 0ml @14:50:13.08 CS.home.seek 0ml @14:50:13.11
Elbow.reconfigure :loose @14:50:13.21 Elbow.home.seek :clear @14:50:16.52 SE.home.seek :down @14:50:17.13 SC.reconfigure :tight @14:50:17.18 SC.home.seek :initial @14:50:20.92 PS.stop @14:50:45.52
SC.configure :limp @14:50:45.57 Forearm.seek :retract @14:50:45.64 Elbow.seek :hammer @14:50:46.82 Forearm.reconfigure :tight @14:50:46.87 Forearm.seek retracted @14:50:47.15 PC.home.seek :open @14:50:47.17
CC.home.seek :open @14:50:50.69 Phase [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] @14:50:50.70 Notice.email "At 14:50:50 began in tube 2: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_101223.rb:14\n{1 => 28,\n 2 => 5,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@14:50:50, 27% flow, 17.8C, 17% humidity, 14.6psia, 13.730V, 0.254A, 138.000Ah, 3.49W",Subject: "Started DA_WCR_101223 phase initialPurge; da [1000, 1000] #da and wsr are run simultanelously" @14:50:50.73
Starting initialPurge @14:50:50.75 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:50:50.82 Hand.open @14:50:51.62 Elbow.reconfigure :tight @14:50:51.69 Elbow.seek FlushPuck::Garage @14:50:53.66 Elbow.reconfigure :loose @14:50:53.81 Forearm.reconfigure :loose @14:50:53.86 Forearm.seek :garage @14:50:54.82 Hand.close @14:50:54.99 upsync @14:50:55.62
Forearm.seek :retract @14:50:56.59 Elbow.reconfigure :tight @14:50:56.64 Elbow.seek CC @14:50:59.58 Elbow.reconfigure :loose @14:50:59.69 Forearm.reconfigure :tight @14:50:59.74 Forearm.seek CC @14:51:00.98 CC.seek :closed @14:51:05.52 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.36 @14:51:05.58 Hand.open @14:51:06.31 Forearm.reconfigure :loose @14:51:06.35 Forearm.seek :retract @14:51:07.32 Flushing the Collection Valve::Manifold @14:51:07.45 CSV.dial CSR @14:51:08.85 Pulling 4.999ml of CSR.flush @14:51:08.86 CSR.select :flush @14:51:08.98 CS.reconfigure :default @14:51:09.02 CS.seek 5ml @14:51:23.29 delay 5 seconds @14:51:28.30 CSR.select :air @14:51:28.48 CS.reconfigure :fast @14:51:28.52 CS.seek 7.5ml @14:51:35.32 CSV.dial :waste @14:51:36.60 CS.reconfigure :default @14:51:36.65 CS.seek 0ml @14:51:56.72 Purging external Intake @14:51:56.85 CSV.dial :flush @14:51:57.31 CS.reconfigure :fast @14:51:57.35 CS.seek 10ml @14:52:21.94 delay 5 seconds @14:52:26.96 CSV.dial CBV @14:52:27.01
CBV.dial CSV @14:52:27.72 CTV.dial :intake @14:52:28.52 Intake.select :open @14:52:28.63 CS.seek 0ml @14:52:52.28 Intake.select :close @14:52:52.39 CTV.dial :air @14:52:53.18 Flushing upper manifold @14:52:53.29 CSV.dial :flush @14:52:54.03 CS.seek 5ml @14:53:06.94 delay 5 seconds @14:53:11.96 CSV.dial :air @14:53:12.47 CS.seek 7.001ml @14:53:18.00 CSV.dial CBV @14:53:18.03
CBV.dial CSV @14:53:19.08 CTV.dial :air @14:53:19.31 CS.seek 0ml @14:53:36.22 delay 7 seconds @14:53:43.25 CS.seek 3ml @14:53:51.31 CSV.dial :waste @14:53:51.77 CS.seek 0ml @14:53:59.55 CSV.dial :flush @14:54:00.12 CS.seek 3ml @14:54:08.13 delay 5 seconds @14:54:13.15 CSV.dial :air @14:54:13.67 CS.seek 5ml @14:54:19.22 CSV.dial :air @14:54:19.41 CS.seek 0ml @14:54:31.56 delay 7 seconds @14:54:38.59 CS.seek 3ml @14:54:46.69 CSV.dial :waste @14:54:47.41 CS.seek 0ml @14:54:55.13 Garaging FlushPuck in Collection Clamp @14:54:55.20 Elbow.reconfigure :tight @14:54:55.25 Elbow.seek CC @14:54:55.81 Elbow.reconfigure :loose @14:54:55.92 Forearm.seek CC @14:54:56.96 Hand.close @14:54:57.62 CC.seek :open @14:55:02.69 Forearm.seek :retract @14:55:03.71 Elbow.reconfigure :tight @14:55:03.76 Elbow.seek :garage @14:55:06.68 Elbow.reconfigure :loose @14:55:06.78 Forearm.reconfigure :tight @14:55:06.83 Forearm.seek :garage @14:55:07.81 Hand.open @14:55:08.42 Forearm.reconfigure :loose @14:55:08.47 Forearm.seek :retract @14:55:09.49 da sampling at most 1000ml, wcr at most 1000ml @14:55:09.53 Loading sh1 puck from tube 2 into Collection Clamp @14:55:09.55 DAfans.email "Started da in tube 2 at 14:55:09UTC12-Oct-23\n{1 => 28,\n 2 => 5,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@14:50:50, 27% flow, 17.8C, 17% humidity, 14.6psia, 13.730V, 0.254A, 138.000Ah, 3.49W",Subject: "da sampling at most 1000ml, wcr at most 1000ml" @14:55:09.81
SC.reconfigure :tight @14:55:09.88 SC.seek 2 @14:55:13.71 upsync @14:55:16.60
SC.configure :limp @14:55:16.83 Elbow.reconfigure :tight @14:55:16.88 Elbow.seek 2 @14:55:18.78 Forearm.reconfigure :tight @14:55:18.83 Forearm.seek 2 + 450 counts @14:55:19.87 SE.reconfigure :pick @14:55:19.93 SE.seek :beamBreak @14:55:22.82 SE.configure :default @14:55:22.99 Elbow.seek 2 @14:55:23.18 Forearm.seek 2 + 300 counts @14:55:23.44 Forearm.reconfigure :loose @14:55:23.49 Hand.close @14:55:24.13 SE.seek :down @14:55:24.70
Forearm.seek :retract @14:55:25.71 Elbow.seek CC @14:55:26.64 SE.stop @14:55:27.30
Elbow.reconfigure :loose @14:55:27.40 Forearm.reconfigure :tight @14:55:27.45 Forearm.seek CC @14:55:28.68 CC.seek :closed @14:55:33.20 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.28 @14:55:33.21 Hand.open @14:55:33.93 Forearm.reconfigure :loose @14:55:33.98 Forearm.seek :retract @14:55:34.95 collectSample 1000ml w/bubble point of 24psi @14:55:34.97 Priming 7ml @14:55:34.98 Intake.select :close @14:55:34.99 Exhaust.select :close @14:55:35.00 SS.hold @14:55:35.21 Calibrating pressure and temperature sensors @14:55:35.24 CTV.dial :air @14:55:35.25 SSV.dial :spit,avoiding: :exhaust @14:55:35.44 SS.reconfigure :fast @14:55:35.49 SS.seek 5ml @14:55:38.25 SSV.dial CBV @14:55:38.28
CBV.dial SSV @14:55:39.63 delay 3.8 seconds @14:55:43.55 Sensor limits: Intake max 113.4psia; Outlet max 105.6psia @14:55:43.56 SSV.dial :spit @14:55:44.89 SS.seek 0ml @14:55:47.68 SSV.dial CBV @14:55:49.01 CTV.dial :intake @14:55:49.77 SSV.dial CBV @14:55:49.79
CBV.dial SSV @14:55:49.88 Allowing 40s for 7.0ml of remaining 7.0ml @14:55:49.89 Intake.select :open @14:55:52.05 Intake @14.7psia, vacuum from 9.2 to 12.2psi @14:55:52.07 SS.reconfigure :inhaling 2.20ml/s @14:55:52.12 SS.seek 7ml @14:55:58.70 Stroke completed -- waiting for pressure to equalize ... @14:55:58.71 SS.reconfigure :exhale @14:55:58.75 SS.hold @14:56:05.44 Intake.select :close @14:56:05.59 SSV.dial :exhaust @14:56:06.36 Exhaust.select :open @14:56:06.47 SS.seek 0ml @14:56:10.68 Exhaust.select :close @14:56:10.80 Sampling 1000.0ml @14:56:10.81 Intake.select :close @14:56:10.82 Exhaust.select :close @14:56:10.83 SS.reconfigure :default @14:56:10.87 SS.hold @14:56:11.08 CTV.dial :intake @14:56:11.32 SSV.dial CBV @14:56:11.34
CBV.dial SSV @14:56:12.11 Allowing 130s for 25.0ml of remaining 1000.0ml @14:56:12.12 Intake.select :open @14:56:14.28 Intake @15.1psia, vacuum from 9.6 to 12.6psi @14:56:14.30 SS.reconfigure :inhaling 2.20ml/s @14:56:14.35 SS.seek 25ml @14:56:23.72 Vacuum too high -- waiting up to 40 seconds @14:56:23.74 SS.reconfigure :exhale @14:56:23.79 SS.hold @14:56:29.82 Intake @15.7psia, vacuum from 10.0 to 13.0psi @14:56:29.84 SS.reconfigure :inhaling 1.76ml/s @14:56:29.89 SS.seek 25ml @14:56:36.01 Stroke completed -- waiting for pressure to equalize ... @14:56:36.02 SS.reconfigure :exhale @14:56:36.60 SS.hold @14:56:44.06 Intake.select :close @14:56:44.22 SSV.dial :exhaust @14:56:44.98 Exhaust.select :open @14:56:45.09 SS.seek 0ml @14:56:54.04 Exhaust.select :close @14:56:54.15 SSV.dial CBV @14:56:54.18
CBV.dial SSV @14:56:54.95 Allowing 130s for 25.0ml of remaining 975.0ml @14:56:54.96 Intake.select :open @14:56:57.12 Intake @15.4psia, vacuum from 9.9 to 12.9psi @14:56:57.14 SS.reconfigure :inhaling 1.76ml/s @14:56:57.19 SS.seek 25ml @14:57:11.38 Vacuum too high -- waiting up to 40 seconds @14:57:11.39 SS.reconfigure :exhale @14:57:11.44 SS.hold @14:57:17.42 Intake @14.5psia, vacuum from 9.0 to 12.0psi @14:57:17.44 SS.reconfigure :inhaling 1.41ml/s @14:57:17.49 SS.seek 25ml @14:57:21.16 Stroke completed -- waiting for pressure to equalize ... @14:57:21.18 SS.reconfigure :exhale @14:57:21.22 SS.hold @14:57:27.74 Intake.select :close @14:57:27.90 SSV.dial :exhaust @14:57:28.67 Exhaust.select :open @14:57:28.78 SS.seek 0ml @14:57:37.74 Exhaust.select :close @14:57:37.85 SSV.dial CBV @14:57:37.88
CBV.dial SSV @14:57:38.65 Allowing 130s for 25.0ml of remaining 950.0ml @14:57:38.66 Intake.select :open @14:57:40.83 Intake @15.5psia, vacuum from 10.0 to 13.0psi @14:57:40.85 SS.reconfigure :inhaling 1.41ml/s @14:57:40.89 SS.seek 25ml @14:58:01.01 Stroke completed -- waiting for pressure to equalize ... @14:58:01.02 SS.reconfigure :exhale @14:58:01.06 SS.hold @14:58:14.02 Intake.select :close @14:58:14.17 SSV.dial :exhaust @14:58:14.93 Exhaust.select :open @14:58:15.04 SS.seek 0ml @14:58:23.77 Exhaust.select :close @14:58:23.89 SSV.dial CBV @14:58:23.91
CBV.dial SSV @14:58:24.68 Allowing 130s for 25.0ml of remaining 925.0ml @14:58:24.69 Intake.select :open @14:58:26.86 Intake @15.5psia, vacuum from 10.0 to 13.0psi @14:58:26.88 SS.reconfigure :inhaling 1.41ml/s @14:58:26.93 SS.seek 25ml @14:58:47.66 Stroke completed -- waiting for pressure to equalize ... @14:58:47.67 SS.reconfigure :exhale @14:58:47.72 SS.hold @14:59:00.80 Intake.select :close @14:59:00.95 SSV.dial :exhaust @14:59:01.71 Exhaust.select :open @14:59:01.83 SS.seek 0ml @14:59:10.82 Exhaust.select :close @14:59:10.93 SSV.dial CBV @14:59:10.95
CBV.dial SSV @14:59:11.73 Allowing 130s for 25.0ml of remaining 900.0ml @14:59:11.74 Intake.select :open @14:59:13.90 Intake @15.4psia, vacuum from 9.9 to 12.9psi @14:59:13.92 SS.reconfigure :inhaling 1.41ml/s @14:59:13.97 SS.seek 25ml @14:59:34.26 Stroke completed -- waiting for pressure to equalize ... @14:59:34.27 SS.reconfigure :exhale @14:59:34.31 SS.hold @14:59:48.70 Intake.select :close @14:59:48.85 SSV.dial :exhaust @14:59:49.62 Exhaust.select :open @14:59:49.73 SS.seek 0ml @14:59:58.69 Exhaust.select :close @14:59:58.81 SSV.dial CBV @14:59:58.83
CBV.dial SSV @14:59:59.59 Allowing 130s for 25.0ml of remaining 875.0ml @14:59:59.60 Intake.select :open @15:00:01.76 Intake @15.4psia, vacuum from 9.9 to 12.9psi @15:00:01.78 SS.reconfigure :inhaling 1.41ml/s @15:00:01.83 SS.seek 25ml @15:00:20.44 Vacuum too high -- waiting up to 40 seconds @15:00:20.45 SS.reconfigure :exhale @15:00:20.93 SS.hold @15:00:27.52 Intake @14.3psia, vacuum from 8.8 to 11.8psi @15:00:27.54 SS.reconfigure :inhaling 1.13ml/s @15:00:27.59 SS.seek 25ml @15:00:29.93 Stroke completed -- waiting for pressure to equalize ... @15:00:29.94 SS.reconfigure :exhale @15:00:29.98 SS.hold @15:00:36.49 Intake.select :close @15:00:36.64 SSV.dial :exhaust @15:00:37.40 Exhaust.select :open @15:00:37.51 SS.seek 0ml @15:00:46.24 Exhaust.select :close @15:00:46.35 SSV.dial CBV @15:00:46.37
CBV.dial SSV @15:00:47.57 Allowing 130s for 25.0ml of remaining 850.0ml @15:00:47.58 Intake.select :open @15:00:49.74 Intake @15.4psia, vacuum from 9.9 to 12.9psi @15:00:49.76 SS.reconfigure :inhaling 1.13ml/s @15:00:49.81 SS.seek 25ml @15:01:14.57 Stroke completed -- waiting for pressure to equalize ... @15:01:14.58 SS.reconfigure :exhale @15:01:14.62 SS.hold @15:01:27.29 Intake.select :close @15:01:27.44 SSV.dial :exhaust @15:01:28.21 Exhaust.select :open @15:01:28.32 SS.seek 0ml @15:01:37.11 Exhaust.select :close @15:01:37.23 SSV.dial CBV @15:01:37.26
CBV.dial SSV @15:01:38.02 Allowing 130s for 25.0ml of remaining 825.0ml @15:01:38.03 Intake.select :open @15:01:40.20 Intake @15.4psia, vacuum from 9.9 to 12.9psi @15:01:40.22 SS.reconfigure :inhaling 1.13ml/s @15:01:40.27 SS.seek 25ml @15:02:05.17 Stroke completed -- waiting for pressure to equalize ... @15:02:05.18 SS.reconfigure :exhale @15:02:05.23 SS.hold @15:02:19.75 Intake.select :close @15:02:19.90 SSV.dial :exhaust @15:02:20.66 Exhaust.select :open @15:02:20.78 SS.seek 0ml @15:02:29.55 Exhaust.select :close @15:02:29.66 SSV.dial CBV @15:02:29.69
CBV.dial SSV @15:02:30.46 Allowing 130s for 25.0ml of remaining 800.0ml @15:02:30.47 Intake.select :open @15:02:32.63 Intake @15.4psia, vacuum from 9.9 to 12.9psi @15:02:32.65 SS.reconfigure :inhaling 1.13ml/s @15:02:32.70 SS.seek 25ml @15:02:53.81 Vacuum too high -- waiting up to 40 seconds @15:02:53.82 SS.reconfigure :exhale @15:02:53.87 SS.hold @15:03:02.00 Intake @15.1psia, vacuum from 9.6 to 12.6psi @15:03:02.01 SS.reconfigure :inhaling 0.91ml/s @15:03:02.06 SS.seek 25ml @15:03:06.88 Stroke completed -- waiting for pressure to equalize ... @15:03:06.89 SS.reconfigure :exhale @15:03:06.93 SS.hold @15:03:14.37 Intake.select :close @15:03:14.52 SSV.dial :exhaust @15:03:15.29 Exhaust.select :open @15:03:15.41 SS.seek 0ml @15:03:24.17 Exhaust.select :close @15:03:24.29 SSV.dial CBV @15:03:24.31
CBV.dial SSV @15:03:25.08 Allowing 130s for 25.0ml of remaining 775.0ml @15:03:25.09 Intake.select :open @15:03:27.26 Intake @15.4psia, vacuum from 9.9 to 12.9psi @15:03:27.28 SS.reconfigure :inhaling 0.91ml/s @15:03:27.32 SS.seek 25ml @15:03:57.44 Stroke completed -- waiting for pressure to equalize ... @15:03:57.45 SS.reconfigure :exhale @15:03:57.50 SS.hold @15:04:13.80 Intake.select :close @15:04:13.96 SSV.dial :exhaust @15:04:14.72 Exhaust.select :open @15:04:14.84 SS.seek 0ml @15:04:23.83 Exhaust.select :close @15:04:23.94 SSV.dial CBV @15:04:23.96
CBV.dial SSV @15:04:24.74 Allowing 130s for 25.0ml of remaining 750.0ml @15:04:24.75 Intake.select :open @15:04:26.92 Intake @15.3psia, vacuum from 9.8 to 12.8psi @15:04:26.93 SS.reconfigure :inhaling 0.91ml/s @15:04:26.98 SS.seek 25ml @15:04:56.12 Vacuum too high -- waiting up to 40 seconds @15:04:56.13 SS.reconfigure :exhale @15:04:56.18 SS.hold @15:05:06.08 Intake @15.2psia, vacuum from 9.7 to 12.7psi @15:05:06.09 SS.reconfigure :inhaling 0.73ml/s @15:05:06.14 SS.seek 25ml @15:05:06.93 Stroke completed -- waiting for pressure to equalize ... @15:05:06.94 SS.reconfigure :exhale @15:05:06.99 SS.hold @15:05:13.48 Intake.select :close @15:05:13.63 SSV.dial :exhaust @15:05:14.39 Exhaust.select :open @15:05:14.51 SS.seek 0ml @15:05:23.50 Exhaust.select :close @15:05:23.61 SSV.dial CBV @15:05:23.64
CBV.dial SSV @15:05:24.41 Allowing 130s for 25.0ml of remaining 725.0ml @15:05:24.42 Intake.select :open @15:05:26.59 Intake @15.3psia, vacuum from 9.8 to 12.8psi @15:05:26.60 SS.reconfigure :inhaling 0.73ml/s @15:05:26.65 SS.seek 25ml @15:06:02.34 Stroke completed -- waiting for pressure to equalize ... @15:06:02.35 SS.reconfigure :exhale @15:06:02.40 SS.hold @15:06:18.69 Intake.select :close @15:06:18.84 SSV.dial :exhaust @15:06:19.61 Exhaust.select :open @15:06:19.73 SS.seek 0ml @15:06:28.70 Exhaust.select :close @15:06:28.81 SSV.dial CBV @15:06:28.84
CBV.dial SSV @15:06:29.61 Allowing 130s for 25.0ml of remaining 700.0ml @15:06:29.62 Intake.select :open @15:06:31.78 Intake @15.2psia, vacuum from 9.7 to 12.7psi @15:06:31.80 SS.reconfigure :inhaling 0.73ml/s @15:06:31.85 SS.seek 25ml @15:07:07.65 Stroke completed -- waiting for pressure to equalize ... @15:07:07.66 SS.reconfigure :exhale @15:07:07.71 SS.hold @15:07:26.44 Intake.select :close @15:07:26.60 SSV.dial :exhaust @15:07:27.36 Exhaust.select :open @15:07:27.47 SS.seek 0ml @15:07:36.50 Exhaust.select :close @15:07:36.61 SSV.dial CBV @15:07:36.64
CBV.dial SSV @15:07:37.41 Allowing 130s for 25.0ml of remaining 675.0ml @15:07:37.42 Intake.select :open @15:07:39.58 Intake @15.2psia, vacuum from 9.7 to 12.7psi @15:07:39.60 SS.reconfigure :inhaling 0.73ml/s @15:07:39.65 SS.seek 25ml @15:08:13.80 Vacuum too high -- waiting up to 40 seconds @15:08:13.82 SS.reconfigure :exhale @15:08:13.87 SS.hold @15:08:25.12 Intake @15.2psia, vacuum from 9.7 to 12.7psi @15:08:25.14 SS.reconfigure :inhaling 0.59ml/s @15:08:25.18 SS.seek 25ml @15:08:28.03 Stroke completed -- waiting for pressure to equalize ... @15:08:28.04 SS.reconfigure :exhale @15:08:28.08 SS.hold @15:08:37.11 Intake.select :close @15:08:37.27 SSV.dial :exhaust @15:08:38.03 Exhaust.select :open @15:08:38.14 SS.seek 0ml @15:08:47.09 Exhaust.select :close @15:08:47.20 SSV.dial CBV @15:08:47.24
CBV.dial SSV @15:08:47.98 Allowing 130s for 25.0ml of remaining 650.0ml @15:08:47.99 Intake.select :open @15:08:50.16 Intake @15.2psia, vacuum from 9.7 to 12.7psi @15:08:50.18 SS.reconfigure :inhaling 0.59ml/s @15:08:50.23 SS.seek 25ml @15:09:34.76 Stroke completed -- waiting for pressure to equalize ... @15:09:34.77 SS.reconfigure :exhale @15:09:34.82 SS.hold @15:09:53.16 Intake.select :close @15:09:53.31 SSV.dial :exhaust @15:09:54.07 Exhaust.select :open @15:09:54.19 SS.seek 0ml @15:10:02.96 Exhaust.select :close @15:10:03.08 SSV.dial CBV @15:10:03.10
CBV.dial SSV @15:10:03.87 Allowing 130s for 25.0ml of remaining 625.0ml @15:10:03.88 Intake.select :open @15:10:06.05 Intake @15.2psia, vacuum from 9.7 to 12.7psi @15:10:06.07 SS.reconfigure :inhaling 0.59ml/s @15:10:06.12 SS.seek 25ml @15:10:50.99 Stroke completed -- waiting for pressure to equalize ... @15:10:51.00 SS.reconfigure :exhale @15:10:51.05 SS.hold @15:11:13.40 Intake.select :close @15:11:13.55 SSV.dial :exhaust @15:11:14.31 Exhaust.select :open @15:11:14.42 SS.seek 0ml @15:11:23.42 Exhaust.select :close @15:11:23.53 SSV.dial CBV @15:11:23.55
CBV.dial SSV @15:11:24.32 Allowing 130s for 25.0ml of remaining 600.0ml @15:11:24.33 Intake.select :open @15:11:26.50 Intake @15.2psia, vacuum from 9.7 to 12.7psi @15:11:26.52 SS.reconfigure :inhaling 0.59ml/s @15:11:26.57 SS.seek 25ml @15:12:11.05 Stroke completed -- waiting for pressure to equalize ... @15:12:11.06 SS.reconfigure :exhale @15:12:11.11 SS.hold @15:12:37.58 Intake.select :close @15:12:37.74 SSV.dial :exhaust @15:12:38.52 Exhaust.select :open @15:12:38.63 SS.seek 0ml @15:12:47.44 Exhaust.select :close @15:12:47.56 SSV.dial CBV @15:12:47.59
CBV.dial SSV @15:12:48.35 Allowing 130s for 25.0ml of remaining 575.0ml @15:12:48.37 Intake.select :open @15:12:50.53 Intake @15.1psia, vacuum from 9.6 to 12.6psi @15:12:50.55 SS.reconfigure :inhaling 0.59ml/s @15:12:50.60 SS.seek 25ml @15:13:31.08 Vacuum too high -- waiting up to 40 seconds @15:13:31.09 SS.reconfigure :exhale @15:13:31.14 SS.hold @15:13:46.07 Intake @15.4psia, vacuum from 9.9 to 12.9psi @15:13:46.09 SS.reconfigure :inhaling 0.47ml/s @15:13:46.14 SS.seek 25ml @15:13:52.21 Stroke completed -- waiting for pressure to equalize ... @15:13:52.22 SS.reconfigure :exhale @15:13:52.27 SS.hold @15:14:06.64 Intake.select :close @15:14:06.79 SSV.dial :exhaust @15:14:07.55 Exhaust.select :open @15:14:07.67 SS.seek 0ml @15:14:16.40 Exhaust.select :close @15:14:16.51 SSV.dial CBV @15:14:16.53
CBV.dial SSV @15:14:17.31 Allowing 130s for 25.0ml of remaining 550.0ml @15:14:17.32 Intake.select :open @15:14:19.48 Intake @15.2psia, vacuum from 9.7 to 12.7psi @15:14:19.50 SS.reconfigure :inhaling 0.47ml/s @15:14:19.55 SS.seek 25ml @15:15:15.47 Stroke completed -- waiting for pressure to equalize ... @15:15:15.48 SS.reconfigure :exhale @15:15:15.53 SS.hold @15:15:42.22 Intake.select :close @15:15:42.37 SSV.dial :exhaust @15:15:43.14 Exhaust.select :open @15:15:43.25 SS.seek 0ml @15:15:52.30 Exhaust.select :close @15:15:52.41 SSV.dial CBV @15:15:52.43
CBV.dial SSV @15:15:53.21 Allowing 130s for 25.0ml of remaining 525.0ml @15:15:53.22 Intake.select :open @15:15:55.38 Intake @15.2psia, vacuum from 9.7 to 12.7psi @15:15:55.40 SS.reconfigure :inhaling 0.47ml/s @15:15:55.45 SS.seek 25ml @15:16:50.61 Stroke completed -- waiting for pressure to equalize ... @15:16:50.62 SS.reconfigure :exhale @15:16:50.67 SS.hold @15:17:21.57 Intake.select :close @15:17:21.72 SSV.dial :exhaust @15:17:22.49 Exhaust.select :open @15:17:22.60 SS.seek 0ml @15:17:31.63 Exhaust.select :close @15:17:31.74 SSV.dial CBV @15:17:31.78
CBV.dial SSV @15:17:32.52 Allowing 130s for 25.0ml of remaining 500.0ml @15:17:32.53 Intake.select :open @15:17:34.70 Intake @15.2psia, vacuum from 9.7 to 12.7psi @15:17:34.71 SS.reconfigure :inhaling 0.47ml/s @15:17:34.76 SS.seek 25ml @15:18:30.03 Stroke completed -- waiting for pressure to equalize ... @15:18:30.04 SS.reconfigure :exhale @15:18:30.09 SS.hold @15:19:05.07 Intake.select :close @15:19:05.23 SSV.dial :exhaust @15:19:05.98 Exhaust.select :open @15:19:06.10 SS.seek 0ml @15:19:15.22 Exhaust.select :close @15:19:15.33 SSV.dial CBV @15:19:15.79
CBV.dial SSV @15:19:16.55 Allowing 130s for 25.0ml of remaining 475.0ml @15:19:16.56 Intake.select :open @15:19:18.72 Intake @15.2psia, vacuum from 9.7 to 12.7psi @15:19:18.74 SS.reconfigure :inhaling 0.47ml/s @15:19:18.79 SS.seek 25ml @15:20:05.29 Vacuum too high -- waiting up to 40 seconds @15:20:05.30 SS.reconfigure :exhale @15:20:05.35 SS.hold @15:20:23.71 Intake @15.4psia, vacuum from 9.9 to 12.9psi @15:20:23.72 SS.reconfigure :inhaling 0.38ml/s @15:20:23.77 SS.seek 25ml @15:20:34.32 Stroke completed -- waiting for pressure to equalize ... @15:20:34.33 SS.reconfigure :exhale @15:20:34.38 SS.hold @15:20:57.27 Intake.select :close @15:20:57.43 SSV.dial :exhaust @15:20:58.19 Exhaust.select :open @15:20:58.30 SS.seek 0ml @15:21:07.12 Exhaust.select :close @15:21:07.23 SSV.dial CBV @15:21:07.26
CBV.dial SSV @15:21:08.03 Allowing 130s for 25.0ml of remaining 450.0ml @15:21:08.04 Intake.select :open @15:21:10.20 Intake @15.1psia, vacuum from 9.6 to 12.6psi @15:21:10.22 SS.reconfigure :inhaling 0.38ml/s @15:21:10.27 SS.seek 25ml @15:22:19.54 Stroke completed -- waiting for pressure to equalize ... @15:22:19.55 SS.reconfigure :exhale @15:22:19.60 SS.hold @15:22:56.71 Intake.select :close @15:22:56.86 SSV.dial :exhaust @15:22:57.62 Exhaust.select :open @15:22:57.73 SS.seek 0ml @15:23:06.56 Exhaust.select :close @15:23:06.67 SSV.dial CBV @15:23:06.69
CBV.dial SSV @15:23:07.47 Allowing 130s for 25.0ml of remaining 425.0ml @15:23:07.48 Intake.select :open @15:23:09.64 Intake @15.2psia, vacuum from 9.7 to 12.7psi @15:23:09.66 SS.reconfigure :inhaling 0.38ml/s @15:23:09.71 SS.seek 25ml @15:24:15.59 Vacuum too high -- waiting up to 40 seconds @15:24:15.61 SS.reconfigure :exhale @15:24:15.66 SS.hold @15:24:36.48 Intake @15.4psia, vacuum from 9.9 to 12.9psi @15:24:36.50 SS.reconfigure :inhaling 0.31ml/s @15:24:36.55 SS.seek 25ml @15:24:41.21 Stroke completed -- waiting for pressure to equalize ... @15:24:41.22 SS.reconfigure :exhale @15:24:41.27 SS.hold @15:25:02.06 Intake.select :close @15:25:02.22 SSV.dial :exhaust @15:25:02.99 Exhaust.select :open @15:25:03.10 SS.seek 0ml @15:25:11.94 Exhaust.select :close @15:25:12.06 SSV.dial CBV @15:25:12.09
CBV.dial SSV @15:25:12.85 Allowing 130s for 25.0ml of remaining 400.0ml @15:25:12.86 Intake.select :open @15:25:15.03 Intake @15.2psia, vacuum from 9.7 to 12.7psi @15:25:15.05 SS.reconfigure :inhaling 0.31ml/s @15:25:15.10 SS.seek 25ml @15:26:45.54 Stroke completed -- waiting for pressure to equalize ... @15:26:45.55 SS.reconfigure :exhale @15:26:45.59 SS.hold @15:27:22.37 Intake.select :close @15:27:22.52 SSV.dial :exhaust @15:27:23.29 Exhaust.select :open @15:27:23.40 SS.seek 0ml @15:27:32.44 Exhaust.select :close @15:27:32.55 SSV.dial CBV @15:27:32.58
CBV.dial SSV @15:27:33.35 Allowing 130s for 25.0ml of remaining 375.0ml @15:27:33.36 Intake.select :open @15:27:35.52 Intake @15.1psia, vacuum from 9.6 to 12.6psi @15:27:35.54 SS.reconfigure :inhaling 0.31ml/s @15:27:35.59 SS.seek 25ml @15:29:06.10 Stroke completed -- waiting for pressure to equalize ... @15:29:06.11 SS.reconfigure :exhale @15:29:06.15 SS.hold @15:29:43.37 SS.reconfigure :default @15:29:43.42 SS.hold @15:29:43.49 Intake.select :close @15:29:43.64 ShallowSampler::Clogged in MAIN -- Filtering too Slowly after 650.0ml @15:29:43.65 SSV.dial :exhaust @15:29:44.41 Exhaust.select :open @15:29:44.52 SS.reconfigure :exhale @15:29:44.57 SS.seek 0ml @15:29:53.63 Exhaust.select :close @15:29:53.74 Sampled 650.0ml @15:29:53.75 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @15:29:53.76 Intake.select :close @15:29:53.83 CSV.dial :waste @15:29:53.97 SSV.dial CBV,avoiding: :exhaust @15:29:54.01 CS.seek 0ml @15:29:54.04
CBV.dial SSV @15:29:54.14 CSV.dial :air @15:29:54.88 CS.seek 10ml @15:29:56.01
CTV.dial :air @15:29:56.78 delay 15 seconds @15:30:11.80 SSV.dial :spit,avoiding: :exhaust @15:30:13.12 CTV.dial CSV,avoiding: :intake @15:30:13.15
SS.seek 0ml @15:30:13.28 SSV.dial CBV,avoiding: :exhaust @15:30:19.75 SSV.dial between CBV and plug @15:30:20.24 CBV.dial between SSV and waste2 @15:30:20.64 CTV.dial CSV,avoiding: :air @15:30:20.67
CSV.dial CTV @15:30:21.19 CS.reconfigure :pressurize @15:30:21.24 CS.seek 0ml @15:30:39.88 BubblePoint: Intake @24.3psia, Outlet @0.5psia @15:30:39.89 CBV.dial SSV @15:30:40.22 SS.reconfigure :default @15:30:40.27 SS.seek 10ml @15:30:45.75 delay 15 seconds @15:31:00.88 Evacuated: Intake @23.9psia, Outlet @23.9psia @15:31:00.90 SSV.dial :spit,avoiding: :exhaust @15:31:01.93 SS.reconfigure :exhale @15:31:01.98 SS.seek 0ml @15:31:07.25 SS.reconfigure :fast @15:31:07.30 SS.seek 5ml @15:31:10.18 SSV.dial CBV,avoiding: :exhaust @15:31:10.21
CBV.dial SSV @15:31:11.55 CTV.dial :air,avoiding: :intake @15:31:12.86 CSV.dial CTV @15:31:12.91
SS.seek 10ml @15:31:14.06 CSV.dial :air @15:31:14.50 CS.reconfigure :fast @15:31:14.55 CS.seek 0ml @15:31:15.61
SSV.dial :spit,avoiding: :exhaust @15:31:16.93 SS.reconfigure :exhale @15:31:16.98 SS.seek 0ml @15:31:22.01 SS.reconfigure :fast @15:31:22.06 SS.seek 5ml @15:31:22.12 CS.stop @15:31:25.04 SSV.dial CBV,avoiding: :exhaust @15:31:25.06
CBV.dial SSV @15:31:27.45 SSV.dial :spit,avoiding: :exhaust @15:31:28.76 SS.reconfigure :default @15:31:28.81 SS.seek 0ml @15:31:31.86 SS.stop @15:31:31.90 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @15:31:31.91 Intake.select :close @15:31:32.40 CSV.dial :waste @15:31:32.55 SSV.dial CBV,avoiding: :exhaust @15:31:32.58
CBV.dial SSV @15:31:33.19 CS.seek 0ml @15:31:33.25 CSV.dial :air @15:31:33.91
CTV.dial :air @15:31:33.99 delay 15 seconds @15:31:34.02 CS.seek 10ml @15:31:49.03
SSV.dial :spit,avoiding: :exhaust @15:31:50.37 CTV.dial CSV,avoiding: :intake @15:31:50.40
SS.reconfigure :exhale @15:31:50.51 SS.seek 0ml @15:31:50.88 SSV.dial CBV,avoiding: :exhaust @15:31:58.72 SSV.dial between CBV and plug @15:31:59.19 CBV.dial between SSV and waste2 @15:31:59.60 CSV.dial between air and CTV @15:31:59.91 CS.seek 7.481ml @15:32:06.44 CTV.dial CSV,avoiding: :air @15:32:06.47
CSV.dial CTV @15:32:06.83 CS.reconfigure :pressurize @15:32:06.88 CS.seek 0ml @15:32:22.57 BubblePoint: Intake @24.2psia, Outlet @3.6psia @15:32:22.58 CBV.dial SSV @15:32:22.92 SS.reconfigure :default @15:32:22.97 SS.seek 10ml @15:32:28.53 delay 15 seconds @15:32:43.66 Evacuated: Intake @24.0psia, Outlet @24.1psia @15:32:43.68 SSV.dial :spit,avoiding: :exhaust @15:32:44.71 SS.reconfigure :exhale @15:32:44.76 SS.seek 0ml @15:32:49.76 SS.reconfigure :fast @15:32:49.81 SS.seek 5ml @15:32:52.63 SSV.dial CBV,avoiding: :exhaust @15:32:52.66
CBV.dial SSV @15:32:53.99 CTV.dial :air,avoiding: :intake @15:32:55.32 CSV.dial CTV @15:32:55.38
SS.seek 10ml @15:32:56.55 CSV.dial :air @15:32:56.98 CS.reconfigure :fast @15:32:57.03 CS.seek 0ml @15:32:57.92
SSV.dial :spit,avoiding: :exhaust @15:32:59.24 SS.reconfigure :exhale @15:32:59.29 SS.seek 0ml @15:33:01.49 CS.stop @15:33:04.28
SS.reconfigure :fast @15:33:04.33 SS.seek 5ml @15:33:07.08 SSV.dial CBV,avoiding: :exhaust @15:33:07.11
CBV.dial SSV @15:33:09.48 SSV.dial :spit,avoiding: :exhaust @15:33:10.79 SS.reconfigure :default @15:33:10.84 SS.seek 0ml @15:33:14.08 SS.stop @15:33:14.12 Lysing: Adding 2.0ml AqMeOH @15:33:14.13 CBV.dial between CSV and waste1,avoiding: :DBV @15:33:15.62 CTV.dial CSV @15:33:16.93 CSV.dial CSR @15:33:17.72 Pulling 1ml of CSR.AqMeOH @15:33:17.73 CSR.select :AqMeOH @15:33:17.85 CS.reconfigure :default @15:33:17.90 CS.seek 1ml @15:33:21.31 delay 5 seconds @15:33:26.32 CSR.select :air @15:33:26.50 CS.seek 6ml @15:33:40.12 delay 2 seconds @15:33:42.14 CSV.dial :waste2 @15:33:42.58 CS.reconfigure :fast @15:33:42.63 CS.seek 0ml @15:33:56.99 CSV.dial CSR @15:33:57.45 Pulling 2ml of CSR.AqMeOH @15:33:57.46 CSR.select :AqMeOH @15:33:57.57 CS.reconfigure :default @15:33:57.62 CS.seek 2ml @15:34:03.56 delay 5 seconds @15:34:08.57 CSR.select :air @15:34:08.74 CS.seek 5ml @15:34:17.43 delay 2 seconds @15:34:19.45 CSV.dial CTV @15:34:19.89 CS.seek 1ml @15:34:31.07 Before lysing Collection Puck Heater at 16.8C/29.7psia @15:34:31.09 Heating Puck.from 2, :sh1 in Collection Clamp to 70C for 10:00 @15:34:31.11 CH.seek 70C,8:00 @15:36:42.76 CH.hold 10:00,70C @15:36:42.97 Begun lysing Collection Puck Heater at 68.1C/36.0psia @15:46:42.98 Ended lysing Collection Puck Heater at 70C/35.3psia @15:46:43.00 CS.seek 0ml @15:46:46.39 CTV.dial between spare and air,avoiding: :intake @15:46:47.90 CSV.dial :air @15:46:48.33 CS.seek 5ml @15:47:01.92 CSV.dial CTV @15:47:02.37 delay 2 seconds @15:47:04.39 CSV.dial :air @15:47:04.84 delay 2 seconds @15:47:06.86 CSV.dial CTV @15:47:07.31 delay 2 seconds @15:47:09.33 CSV.dial :air @15:47:09.78 delay 2 seconds @15:47:11.80 CS.seek 0ml @15:47:25.41 Puck @80.3psia after final lysis pressurization @15:47:25.42 Recovering Lysate into Collection syringe @15:47:25.43 CSV.dial CBV @15:47:26.45 CBV.dial CSV @15:47:26.84 CS.seek 5ml @15:47:40.64 delay 5 seconds @15:47:45.66 CTV.dial :air @15:47:46.18 CS.seek 7ml @15:47:52.14 delay 5 seconds @15:47:57.16 CSV.dial :air2 @15:47:57.59 CS.seek 10ml @15:48:06.26 CSV.dial between air and CTV,avoiding: CBV @15:48:07.42 Lysing Complete @15:48:07.49 Storing sh1 puck in Collection Clamp to tube 1 @15:48:07.67 SC.reconfigure :tight @15:48:07.72 SC.seek 1 @15:48:11.41 SC.configure :limp @15:48:11.63 Elbow.reconfigure :tight @15:48:11.68 Elbow.seek 1 @15:48:13.47 Forearm.reconfigure :tight @15:48:13.52 Forearm.seek 1 + 450 counts @15:48:14.55 SE.reconfigure :pick @15:48:14.61 SE.seek :beamBreak @15:48:15.96 SE.configure :default @15:48:16.12 Elbow.reconfigure :limp @15:48:16.17 Forearm.reconfigure :loose @15:48:16.22 Forearm.seek 1 @15:48:16.52 Elbow.hold @15:48:16.58 Elbow.reconfigure :tight @15:48:17.11 SE.seek 27.04 Pucks below Top Plate @15:48:17.69 Forearm.force -20 @15:48:17.74 Forearm.seek 1 + 277 counts @15:48:18.02 Forearm.reconfigure :tight @15:48:18.07 Forearm.seek 1 + 450 counts @15:48:18.26 SE.seek 28.04 Pucks below Top Plate @15:48:18.77 Forearm.reconfigure :loose @15:48:18.82 Forearm.seek :retract @15:48:19.74 Elbow.seek CC @15:48:21.44 Elbow.reconfigure :loose @15:48:21.55 Forearm.seek CC @15:48:22.52 Hand.close @15:48:23.08 CC.seek :open @15:48:27.81 Forearm.seek :retract @15:48:28.84 Elbow.reconfigure :tight @15:48:28.89 Elbow.seek 1 @15:48:30.67 Elbow.reconfigure :limp @15:48:30.72 Forearm.seek 1 @15:48:31.71 Elbow.hold @15:48:31.78 Elbow.reconfigure :tight @15:48:31.83 Hand.open @15:48:32.56 Forearm.force -20 @15:48:32.61 Forearm.seek 1 + 308 counts @15:48:32.91 Forearm.reconfigure :tight @15:48:32.96 Forearm.seek 1 + 450 counts @15:48:33.23 SE.seek :down @15:48:34.06 SE.stop @15:48:34.73
Forearm.reconfigure :loose @15:48:34.85 Forearm.seek :retract @15:48:35.78 Tranferring DA @15:48:35.79 Beginning samplePassC @15:48:35.80 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @15:48:36.00 Elbow.seek FlushPuck::Garage @15:48:37.85 Elbow.reconfigure :loose @15:48:37.96 Forearm.seek :garage @15:48:38.98 Hand.close @15:48:39.69 Forearm.seek :retract @15:48:40.70 Elbow.reconfigure :tight @15:48:40.75 Elbow.seek CC @15:48:43.71 Elbow.reconfigure :loose @15:48:43.81 Forearm.reconfigure :tight @15:48:43.86 Forearm.seek CC @15:48:45.08 CC.seek :closed @15:48:49.61 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.42 @15:48:49.67 Hand.open @15:48:50.38 Forearm.reconfigure :loose @15:48:50.43 Forearm.seek :retract @15:48:51.40 PSV.dial PSR @15:48:52.79 Pulling 1.5ml of PSR.flush @15:48:52.80 PSR.select :flush @15:48:52.92 PS.seek 1.5ml @15:49:09.05 delay 5 seconds @15:49:14.07 PSV.dial :waste1 @15:49:14.54 PS.seek 0ml @15:49:31.18 PSV.dial PSR @15:49:31.66 PSR.select :air @15:49:31.77 PS.seek 2.5ml @15:49:58.18 PRV.dial CBV,avoiding: :ab2 @15:49:58.20
CBV.dial PRV @15:49:59.95 PSV.dial :mixloop @15:50:01.11 CTV.dial :air,avoiding: :intake @15:50:01.35 PTV.dial :PRVmixing @15:50:02.73 PS.seek 0ml @15:50:29.29 PSV.dial :air @15:50:29.90 PS.seek 2.5ml @15:50:56.29 PSV.dial :mixloop @15:50:56.75 PS.seek 0ml @15:51:23.11 PSV.dial :air @15:51:23.58 PS.seek 2.5ml @15:51:49.95 PSV.dial :mixloop @15:51:50.43 PS.seek 0ml @15:52:16.76 Garaging FlushPuck in Collection Clamp @15:52:16.83 Elbow.reconfigure :tight @15:52:16.88 Elbow.seek CC @15:52:17.20 Elbow.reconfigure :loose @15:52:17.30 Forearm.seek CC @15:52:18.36 Hand.close @15:52:18.95 CC.seek :open @15:52:23.57 Forearm.seek :retract @15:52:24.57 Elbow.reconfigure :tight @15:52:24.62 Elbow.seek :garage @15:52:27.58 Elbow.reconfigure :loose @15:52:27.68 Forearm.reconfigure :tight @15:52:27.73 Forearm.seek :garage @15:52:28.79 Hand.open @15:52:29.50 Forearm.reconfigure :loose @15:52:29.55 Forearm.seek :retract @15:52:30.53 PSV.dial :mixloop @15:52:30.61 PTV.dial :PRVmixing @15:52:31.13 PRV.dial CBV @15:52:31.15
CBV.dial PRV @15:52:31.24 CTV.dial :air,avoiding: :intake @15:52:31.32 Loading lyfil puck from tube 2 into Collection Clamp @15:52:31.56 SC.reconfigure :tight @15:52:31.61 SC.seek 2 @15:52:35.20 SC.configure :limp @15:52:35.42 Elbow.reconfigure :tight @15:52:35.47 Elbow.seek 2 @15:52:37.50 Forearm.reconfigure :tight @15:52:37.55 Forearm.seek 2 + 450 counts @15:52:38.58 SE.reconfigure :pick @15:52:38.64 SE.seek :beamBreak @15:52:41.64 SE.configure :default @15:52:41.80 Elbow.seek 2 @15:52:41.99 Forearm.seek 2 + 300 counts @15:52:42.26 Forearm.reconfigure :loose @15:52:42.30 Hand.close @15:52:42.95 SE.seek :down @15:52:43.15
Forearm.seek :retract @15:52:44.15 Elbow.seek CC @15:52:45.54 SE.stop @15:52:45.76
Elbow.reconfigure :loose @15:52:45.87 Forearm.reconfigure :tight @15:52:45.91 Forearm.seek CC @15:52:47.60 CC.seek :closed @15:52:52.11 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.09 @15:52:52.13 Hand.open @15:52:52.76 Forearm.reconfigure :loose @15:52:52.80 Forearm.seek :retract @15:52:53.77 CTV.dial CSV,avoiding: :intake @15:52:55.10 CSV.dial CTV @15:52:55.43 PS.seek 2.5ml @15:53:21.95 CS.seek 4.5ml @15:53:36.85 delay 8 seconds @15:53:44.87 PSV.dial :air @15:53:45.34 delay 2 seconds @15:53:47.36 PSV.dial :waste2 @15:53:48.10 PS.seek 0ml @15:54:14.42 CBV.dial between PRV and waste1 @15:54:14.74 CSV.dial :air @15:54:15.21 CS.seek 10ml @15:54:30.07 CSV.dial CTV @15:54:30.53 CS.seek 0ml @15:54:57.13 CBV.dial :waste1 @15:54:57.43 delay 5 seconds @15:55:02.45 CTV.dial :air,avoiding: :intake @15:55:03.86 Storing lyfil puck in Collection Clamp to tube 1 @15:55:04.04 SC.reconfigure :tight @15:55:04.09 SC.seek 1 @15:55:07.74 SC.configure :limp @15:55:07.96 Elbow.reconfigure :tight @15:55:08.01 Elbow.seek 1 @15:55:09.76 Forearm.reconfigure :tight @15:55:09.81 Forearm.seek 1 + 450 counts @15:55:11.33 SE.reconfigure :pick @15:55:11.39 SE.seek :beamBreak @15:55:12.60 SE.configure :default @15:55:12.77 Elbow.reconfigure :limp @15:55:12.82 Forearm.reconfigure :loose @15:55:12.87 Forearm.seek 1 @15:55:13.13 Elbow.hold @15:55:13.19 Elbow.reconfigure :tight @15:55:13.29 SE.seek 28.03 Pucks below Top Plate @15:55:13.86 Forearm.force -20 @15:55:13.90 Forearm.seek 1 + 312 counts @15:55:14.31 Forearm.reconfigure :tight @15:55:14.36 Forearm.seek 1 + 450 counts @15:55:14.60 SE.seek 29.03 Pucks below Top Plate @15:55:15.23 Forearm.reconfigure :loose @15:55:15.28 Forearm.seek :retract @15:55:16.21 Elbow.seek CC @15:55:17.88 Elbow.reconfigure :loose @15:55:17.98 Forearm.seek CC @15:55:18.95 Hand.close @15:55:19.57 CC.seek :open @15:55:24.30 Forearm.seek :retract @15:55:25.29 Elbow.reconfigure :tight @15:55:25.33 Elbow.seek 1 @15:55:27.13 Elbow.reconfigure :limp @15:55:27.19 Forearm.seek 1 @15:55:28.62 Elbow.hold @15:55:28.76 Elbow.reconfigure :tight @15:55:28.81 Hand.open @15:55:29.57 Forearm.force -20 @15:55:29.61 Forearm.seek 1 + 318 counts @15:55:29.92 Forearm.reconfigure :tight @15:55:29.97 Forearm.seek 1 + 450 counts @15:55:30.24 SE.seek :down @15:55:30.98 SE.stop @15:55:31.38
Forearm.reconfigure :loose @15:55:31.43 Forearm.seek :retract @15:55:32.41 PSV.dial :air @15:55:33.16 PS.seek 2.5ml @15:55:59.51 delay 1 second @15:56:00.52 PSV.dial :mixloop @15:56:00.98 delay 1 second @15:56:01.99 PSV.dial :air @15:56:02.46 delay 1 second @15:56:03.48 PSV.dial :mixloop @15:56:03.95 delay 1 second @15:56:04.96 PSV.dial :air @15:56:05.43 delay 1 second @15:56:06.45 PS.seek 0ml @15:56:32.76 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @15:56:32.95 Elbow.seek FlushPuck::Garage @15:56:34.71 Elbow.reconfigure :loose @15:56:34.82 Forearm.seek :garage @15:56:35.84 Hand.close @15:56:36.56 Forearm.seek :retract @15:56:37.53 Elbow.reconfigure :tight @15:56:37.58 Elbow.seek PC @15:56:38.82 Elbow.reconfigure :loose @15:56:38.93 Forearm.reconfigure :tight @15:56:38.98 Forearm.seek PC @15:56:40.71 PC.seek :closed @15:56:45.38 Processing Clamp onSpring/offSpring for 16/26 samples, on/off torque 1.61 @15:56:45.45 Hand.open @15:56:46.13 Forearm.reconfigure :loose @15:56:46.18 Forearm.seek :retract @15:56:47.17 PRV.dial :air @15:56:47.62 PSV.dial :mixloop @15:56:48.15 PS.seek 0.3ml @15:56:52.01 PSV.dial PSR @15:56:53.05 Pulling 0.4ml of PSR.air @15:56:53.06 PSR.select :air @15:56:53.09 PS.seek 0.7ml @15:56:57.95 delay 3 seconds @15:57:00.96 PSR.select :air @15:57:00.99 PS.seek 2.5ml @15:57:20.23 delay 3 seconds @15:57:23.24 PSV.dial :mixloop @15:57:24.29 PTV.dial :mixing @15:57:25.04 PBV.dial :waste2 @15:57:25.99 PS.seek 0ml @15:57:52.45 Garaging FlushPuck in Processing Clamp @15:57:52.52 Elbow.reconfigure :tight @15:57:52.57 Elbow.seek PC @15:57:52.69 Elbow.reconfigure :loose @15:57:52.79 Forearm.seek PC @15:57:53.75 Hand.close @15:57:54.32 PC.seek :open @15:57:59.03 Forearm.seek :retract @15:58:00.00 Elbow.reconfigure :tight @15:58:00.05 Elbow.seek :garage @15:58:01.87 Elbow.reconfigure :loose @15:58:01.98 Forearm.reconfigure :tight @15:58:02.02 Forearm.seek :garage @15:58:03.08 Hand.open @15:58:03.75 Forearm.reconfigure :loose @15:58:03.79 Forearm.seek :retract @15:58:04.80 Loading da puck from tube 2 into Processing Clamp @15:58:05.04 SC.reconfigure :tight @15:58:05.09 SC.seek 2 @15:58:08.66 SC.configure :limp @15:58:08.88 Elbow.reconfigure :tight @15:58:08.93 Elbow.seek 2 @15:58:10.90 Forearm.reconfigure :tight @15:58:10.95 Forearm.seek 2 + 450 counts @15:58:11.98 SE.reconfigure :pick @15:58:12.04 SE.seek :beamBreak @15:58:15.00 SE.configure :default @15:58:15.16 Elbow.seek 2 @15:58:15.43 Forearm.seek 2 + 300 counts @15:58:15.56 Forearm.reconfigure :loose @15:58:15.61 Hand.close @15:58:16.20 SE.seek :down @15:58:16.40
Forearm.seek :retract @15:58:17.39 Elbow.seek PC @15:58:18.88 SE.stop @15:58:19.90
Elbow.reconfigure :loose @15:58:20.00 Forearm.reconfigure :tight @15:58:20.05 Forearm.seek PC @15:58:21.79 PC.seek :closed @15:58:26.43 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.63 @15:58:26.44 Hand.open @15:58:27.12 Forearm.reconfigure :loose @15:58:27.17 Forearm.seek :retract @15:58:28.13 Heating Puck.from 2, :da in Processing Clamp to 28C for 2:00:00 @15:58:28.15 PH.seek 28C,5:00 @15:59:09.82 PH.hold 2:00:00,28C @15:59:09.86 delay 1:30 @16:00:39.92 PSR.select :air @16:00:39.94 PSV.dial PSR @16:00:40.99 Pulling 0.5ml of PSR.wash @16:00:41.00 PSR.select :wash @16:00:41.12 PS.seek 0.5ml @16:00:47.13 delay 5 seconds @16:00:52.15 PSR.select :air @16:00:52.27 PS.seek 2ml @16:01:08.44 PTV.dial :puck @16:01:09.19 PSV.dial PTV @16:01:10.07 PBV.dial :waste1 @16:01:11.47 PS.seek 1.5ml @16:01:17.47 delay 3 seconds @16:01:20.49 PBV.dial between PSV and waste1 @16:01:20.84 PS.seek 0ml @16:01:37.00 delay 5:00 @16:06:37.02 recoverPSRreagent08 @16:06:37.03 PTV.dial :puck @16:06:37.11 PBV.dial :waste1 @16:06:37.47 delay 30 seconds @16:07:07.49 PBV.dial between PSV and waste1 @16:07:07.85 PSR.select :air @16:07:07.87 PSV.dial PSR @16:07:08.73 PS.reconfigure :fast @16:07:08.78 PS.seek 2.5ml @16:07:32.84 PSV.dial PTV @16:07:33.58 delay 5 seconds @16:07:38.60 PSV.dial PSR @16:07:39.34 delay 5 seconds @16:07:44.36 PSV.dial PTV @16:07:45.11 delay 5 seconds @16:07:50.13 PSV.dial :air @16:07:51.00 PS.seek 0ml @16:08:14.50 PTV.dial :PRVmixing,via: PRV @16:08:15.82 PSV.dial :mixloop @16:08:16.28 PRV.dial :ab1 @16:08:17.01 PS.reconfigure :slow @16:08:17.06 PS.seek 0.15ml @16:08:20.59 delay 2 seconds @16:08:22.61 CBV.dial PRV @16:08:22.64
PRV.dial CBV @16:08:23.20 PS.seek 0.35ml @16:08:27.84 delay 2 seconds @16:08:29.86 CBV.dial between PRV and PSV @16:08:30.18 PRV.dial :air @16:08:30.64 PS.seek 0.9ml @16:08:42.43 PRV.dial :waste2 @16:08:43.25 PS.seek 0.5ml @16:08:51.90 addPMLReagent08 @16:08:51.91 PSV.dial :air @16:08:52.39 PS.reconfigure :default @16:08:52.44 PS.seek 2ml @16:09:08.74 PSV.dial :PML @16:09:09.21 PTV.dial :mixing @16:09:09.96 PBV.dial :waste1 @16:09:10.39 PS.seek 1.85ml @16:09:12.84 PBV.dial between PSV and waste1 @16:09:13.20 PS.seek 0ml @16:09:32.90 delay 2 seconds @16:09:34.92 Beginning samplePassC @16:09:34.93 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @16:09:35.11 Elbow.reconfigure :tight @16:09:35.16 Elbow.seek FlushPuck::Garage @16:09:36.51 Elbow.reconfigure :loose @16:09:36.62 Forearm.seek :garage @16:09:37.57 Hand.close @16:09:38.30 Forearm.seek :retract @16:09:39.27 Elbow.reconfigure :tight @16:09:39.32 Elbow.seek CC @16:09:42.23 Elbow.reconfigure :loose @16:09:42.34 Forearm.reconfigure :tight @16:09:42.38 Forearm.seek CC @16:09:43.72 CC.seek :closed @16:09:48.25 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.41 @16:09:48.76 Hand.open @16:09:49.49 Forearm.reconfigure :loose @16:09:49.54 Forearm.seek :retract @16:09:50.52 PSV.dial PSR @16:09:51.70 Pulling 1.5ml of PSR.flush @16:09:51.71 PSR.select :flush @16:09:51.83 PS.seek 1.5ml @16:10:07.96 delay 5 seconds @16:10:12.98 PSV.dial :waste1 @16:10:13.47 PS.seek 0ml @16:10:29.68 PSV.dial PSR @16:10:30.15 PSR.select :air @16:10:30.27 PS.seek 2.5ml @16:10:56.59 PRV.dial CBV,avoiding: :ab2 @16:10:56.62
CBV.dial PRV @16:10:58.24 PSV.dial :mixloop @16:10:59.40 CTV.dial :air,avoiding: :intake @16:10:59.61 PTV.dial :PRVmixing @16:11:00.39 PS.seek 0ml @16:11:26.71 PSV.dial :air @16:11:27.31 PS.seek 2.5ml @16:11:53.74 PSV.dial :mixloop @16:11:54.20 PS.seek 0ml @16:12:20.58 PSV.dial :air @16:12:21.06 PS.seek 2.5ml @16:12:47.46 PSV.dial :mixloop @16:12:47.93 PS.seek 0ml @16:13:14.24 Garaging FlushPuck in Collection Clamp @16:13:14.31 Elbow.reconfigure :tight @16:13:14.36 Elbow.seek CC @16:13:14.64 Elbow.reconfigure :loose @16:13:14.74 Forearm.seek CC @16:13:15.77 Hand.close @16:13:16.43 CC.seek :open @16:13:21.07 Forearm.seek :retract @16:13:22.07 Elbow.reconfigure :tight @16:13:22.12 Elbow.seek :garage @16:13:25.01 Elbow.reconfigure :loose @16:13:25.55 Forearm.reconfigure :tight @16:13:25.60 Forearm.seek :garage @16:13:26.58 Hand.open @16:13:27.24 Forearm.reconfigure :loose @16:13:27.29 Forearm.seek :retract @16:13:28.26 delayUntil "16:17:34.91" @16:13:28.28 Whole Cell Archive sampling 1000ml @16:13:28.30 Loading wcr puck from tube 2 into Collection Clamp @16:13:28.61 SC.reconfigure :tight @16:13:28.66 SC.seek 2 @16:13:28.98 SC.configure :limp @16:13:29.21 Elbow.reconfigure :tight @16:13:29.26 Elbow.seek 2 @16:13:31.30 Forearm.reconfigure :tight @16:13:31.35 Forearm.seek 2 + 450 counts @16:13:32.44 SE.reconfigure :pick @16:13:32.50 SE.seek :beamBreak @16:13:35.55 SE.configure :default @16:13:35.71 Elbow.seek 2 @16:13:36.20 Forearm.seek 2 + 300 counts @16:13:36.38 Forearm.reconfigure :loose @16:13:36.43 Hand.close @16:13:37.06 SE.seek :down @16:13:37.27 Forearm.seek :retract @16:13:38.21 Elbow.seek CC @16:13:39.78 SE.stop @16:13:40.44 Elbow.reconfigure :loose @16:13:40.55 Forearm.reconfigure :tight @16:13:40.60 Forearm.seek CC @16:13:41.91 CC.seek :closed @16:13:46.43 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.25 @16:13:46.45 Hand.open @16:13:47.11 Forearm.reconfigure :loose @16:13:47.16 Forearm.seek :retract @16:13:48.11 collectSample 1000ml w/bubble point of 35psi @16:13:48.13 Priming 7ml @16:13:48.14 Intake.select :close @16:13:48.15 Exhaust.select :close @16:13:48.17 SS.hold @16:13:48.38 Calibrating pressure sensors @16:13:48.39 CTV.dial :air @16:13:48.41 SSV.dial :spit,avoiding: :exhaust @16:13:48.49 SS.reconfigure :fast @16:13:48.54 SS.seek 5ml @16:13:51.36 SSV.dial CBV @16:13:51.40 CBV.dial SSV @16:13:52.72 delay 3.8 seconds @16:13:56.65 Sensor limits: Intake max 113.9psia; Outlet max 105.5psia @16:13:56.66 SSV.dial :spit @16:13:57.97 SS.seek 0ml @16:14:00.61 SSV.dial CBV @16:14:01.94 CTV.dial :intake @16:14:02.72 SSV.dial CBV @16:14:02.75 CBV.dial SSV @16:14:02.89 Allowing 40s for 7.0ml of remaining 7.0ml @16:14:02.91 Intake.select :open @16:14:05.08 Intake @14.5psia, vacuum from 9.0 to 12.0psi @16:14:05.10 SS.reconfigure :inhaling 2.20ml/s @16:14:05.59 SS.seek 7ml @16:14:12.57 Stroke completed -- waiting for pressure to equalize ... @16:14:12.59 SS.reconfigure :exhale @16:14:12.64 SS.hold @16:14:23.69 Intake.select :close @16:14:23.85 SSV.dial :exhaust @16:14:24.62 Exhaust.select :open @16:14:24.74 SS.seek 0ml @16:14:28.69 Exhaust.select :close @16:14:28.81 Sampling 1000.0ml @16:14:28.82 Intake.select :close @16:14:28.83 Exhaust.select :close @16:14:28.84 SS.reconfigure :default @16:14:28.89 SS.hold @16:14:29.10 CTV.dial :intake @16:14:29.18 SSV.dial CBV @16:14:29.20 CBV.dial SSV @16:14:29.98 Allowing 130s for 25.0ml of remaining 1000.0ml @16:14:30.00 Intake.select :open @16:14:32.61 Intake @15.3psia, vacuum from 9.8 to 12.8psi @16:14:32.63 SS.reconfigure :inhaling 2.20ml/s @16:14:32.68 SS.seek 25ml @16:14:46.04 Vacuum too high -- waiting up to 40 seconds @16:14:46.05 SS.reconfigure :exhale @16:14:46.11 SS.hold @16:14:54.02 Intake @15.0psia, vacuum from 9.5 to 12.5psi @16:14:54.04 SS.reconfigure :inhaling 1.76ml/s @16:14:54.09 SS.seek 25ml @16:14:56.38 Stroke completed -- waiting for pressure to equalize ... @16:14:56.39 SS.reconfigure :exhale @16:14:56.44 SS.hold @16:15:07.67 Intake.select :close @16:15:07.83 SSV.dial :exhaust @16:15:08.61 Exhaust.select :open @16:15:08.73 SS.seek 0ml @16:15:17.70 Exhaust.select :close @16:15:17.81 SSV.dial CBV @16:15:17.85 CBV.dial SSV @16:15:18.59 Allowing 130s for 25.0ml of remaining 975.0ml @16:15:18.61 Intake.select :open @16:15:20.77 Intake @15.4psia, vacuum from 9.9 to 12.9psi @16:15:20.79 SS.reconfigure :inhaling 1.76ml/s @16:15:20.84 SS.seek 25ml @16:15:37.91 Stroke completed -- waiting for pressure to equalize ... @16:15:37.93 SS.reconfigure :exhale @16:15:37.98 SS.hold @16:15:58.45 Intake.select :close @16:15:58.61 SSV.dial :exhaust @16:15:59.41 Exhaust.select :open @16:15:59.53 SS.seek 0ml @16:16:08.53 Exhaust.select :close @16:16:08.64 SSV.dial CBV @16:16:08.68 CBV.dial SSV @16:16:09.43 Allowing 130s for 25.0ml of remaining 950.0ml @16:16:09.45 Intake.select :open @16:16:11.61 Intake @15.5psia, vacuum from 10.0 to 13.0psi @16:16:11.63 SS.reconfigure :inhaling 1.76ml/s @16:16:11.68 SS.seek 25ml @16:16:28.38 Stroke completed -- waiting for pressure to equalize ... @16:16:28.40 SS.reconfigure :exhale @16:16:28.44 SS.hold @16:16:45.31 Intake.select :close @16:16:45.47 SSV.dial :exhaust @16:16:46.23 Exhaust.select :open @16:16:46.34 SS.seek 0ml @16:16:55.35 Exhaust.select :close @16:16:55.47 SSV.dial CBV @16:16:55.51 CBV.dial SSV @16:16:56.26 Allowing 130s for 25.0ml of remaining 925.0ml @16:16:56.27 Intake.select :open @16:16:58.44 Intake @15.6psia, vacuum from 10.0 to 13.0psi @16:16:58.46 SS.reconfigure :inhaling 1.76ml/s @16:16:58.51 SS.seek 25ml @16:17:14.94 Vacuum too high -- waiting up to 40 seconds @16:17:14.95 SS.reconfigure :exhale @16:17:15.01 SS.hold @16:17:21.05 Intake @15.0psia, vacuum from 9.5 to 12.5psi @16:17:21.07 SS.reconfigure :inhaling 1.41ml/s @16:17:21.12 SS.seek 25ml @16:17:22.47 Stroke completed -- waiting for pressure to equalize ... @16:17:22.49 SS.reconfigure :exhale @16:17:22.54 SS.hold @16:17:31.30 Intake.select :close @16:17:31.45 SSV.dial :exhaust @16:17:32.67 Exhaust.select :open @16:17:32.78 SS.seek 0ml @16:17:34.92 recoverPRVreagent08 @16:17:34.93 PTV.dial :mixing @16:17:35.69 PBV.dial :waste1 @16:17:36.06 delay 30 seconds @16:17:41.73 Exhaust.select :close @16:17:41.84 SSV.dial CBV @16:17:41.88 CBV.dial SSV @16:17:42.64 Allowing 130s for 25.0ml of remaining 900.0ml @16:17:42.65 Intake.select :open @16:17:44.81 Intake @15.6psia, vacuum from 10.0 to 13.0psi @16:17:44.83 SS.reconfigure :inhaling 1.41ml/s @16:17:44.88 SS.seek 25ml @16:18:05.09 Stroke completed -- waiting for pressure to equalize ... @16:18:05.10 SS.reconfigure :exhale @16:18:05.15 SS.hold @16:18:06.09 PBV.dial between PSV and waste1 @16:18:06.47 PSV.dial :air @16:18:06.94 PS.reconfigure :fast @16:18:06.99 PS.seek 2.5ml @16:18:17.86 Intake.select :close @16:18:18.03 SSV.dial :exhaust @16:18:18.79 Exhaust.select :open @16:18:18.91 SS.seek 0ml @16:18:27.69 Exhaust.select :close @16:18:27.81 SSV.dial CBV @16:18:27.85 CBV.dial SSV @16:18:28.60 Allowing 130s for 25.0ml of remaining 875.0ml @16:18:28.62 Intake.select :open @16:18:30.79 Intake @15.6psia, vacuum from 10.0 to 13.0psi @16:18:30.80 SS.reconfigure :inhaling 1.41ml/s @16:18:30.85 SS.seek 25ml @16:18:31.14 PSV.dial :mixloop @16:18:31.61 delay 2 seconds @16:18:33.63 PSV.dial :air @16:18:34.22 delay 2 seconds @16:18:36.24 PSV.dial :mixloop @16:18:36.69 delay 2 seconds @16:18:38.70 PSV.dial :air @16:18:39.18 PS.seek 0ml @16:18:51.49 Stroke completed -- waiting for pressure to equalize ... @16:18:51.50 SS.reconfigure :exhale @16:18:51.55 SS.hold @16:19:03.04 washCycleDA #1 of 2 @16:19:03.05 PSR.select :air @16:19:03.07 PSV.dial PSR @16:19:04.30 Intake.select :close @16:19:04.42 Pulling 0.5ml of PSR.wash @16:19:04.43 PSR.select :wash @16:19:04.54 SSV.dial :exhaust @16:19:04.61 PS.reconfigure :default @16:19:04.66 PS.seek 0.5ml @16:19:05.32 Exhaust.select :open @16:19:05.44 SS.seek 0ml @16:19:10.67 delay 5 seconds @16:19:14.49 Exhaust.select :close @16:19:14.60 SSV.dial CBV @16:19:14.63 CBV.dial SSV @16:19:15.40 Allowing 130s for 25.0ml of remaining 850.0ml @16:19:15.41 Intake.select :open @16:19:15.69 PSR.select :air @16:19:15.81 PS.seek 2ml @16:19:17.58 Intake @15.6psia, vacuum from 10.0 to 13.0psi @16:19:17.60 SS.reconfigure :inhaling 1.41ml/s @16:19:17.65 SS.seek 25ml @16:19:31.98 PTV.dial :puck @16:19:32.73 PSV.dial PTV @16:19:33.48 PBV.dial :waste1 @16:19:33.92 PS.seek 1.5ml @16:19:37.64 Stroke completed -- waiting for pressure to equalize ... @16:19:37.66 SS.reconfigure :exhale @16:19:37.71 SS.hold @16:19:39.82 delay 3 seconds @16:19:42.84 PBV.dial between PSV and waste1 @16:19:43.29 PS.seek 0ml @16:19:52.01 Intake.select :close @16:19:52.17 SSV.dial :exhaust @16:19:52.94 Exhaust.select :open @16:19:53.06 SS.seek 0ml @16:19:59.42 delay 2:00 @16:20:01.85 Exhaust.select :close @16:20:01.96 SSV.dial CBV @16:20:02.00 CBV.dial SSV @16:20:02.74 Allowing 130s for 25.0ml of remaining 825.0ml @16:20:02.76 Intake.select :open @16:20:04.94 Intake @15.4psia, vacuum from 9.9 to 12.9psi @16:20:04.96 SS.reconfigure :inhaling 1.41ml/s @16:20:05.01 SS.seek 25ml @16:20:25.55 Stroke completed -- waiting for pressure to equalize ... @16:20:25.56 SS.reconfigure :exhale @16:20:25.61 SS.hold @16:20:40.53 Intake.select :close @16:20:40.68 SSV.dial :exhaust @16:20:41.46 Exhaust.select :open @16:20:41.57 SS.seek 0ml @16:20:50.60 Exhaust.select :close @16:20:50.71 SSV.dial CBV @16:20:50.75 CBV.dial SSV @16:20:51.49 Allowing 130s for 25.0ml of remaining 800.0ml @16:20:51.50 Intake.select :open @16:20:53.67 Intake @15.4psia, vacuum from 9.9 to 12.9psi @16:20:53.69 SS.reconfigure :inhaling 1.41ml/s @16:20:53.74 SS.seek 25ml @16:21:10.81 Vacuum too high -- waiting up to 40 seconds @16:21:10.83 SS.reconfigure :exhale @16:21:10.88 SS.hold @16:21:18.66 Intake @14.6psia, vacuum from 9.1 to 12.1psi @16:21:18.68 SS.reconfigure :inhaling 1.13ml/s @16:21:18.73 SS.seek 25ml @16:21:22.94 Stroke completed -- waiting for pressure to equalize ... @16:21:22.95 SS.reconfigure :exhale @16:21:23.00 SS.hold @16:21:30.48 Intake.select :close @16:21:30.64 SSV.dial :exhaust @16:21:31.41 Exhaust.select :open @16:21:31.93 SS.seek 0ml @16:21:40.69 Exhaust.select :close @16:21:40.81 SSV.dial CBV @16:21:40.84 CBV.dial SSV @16:21:41.61 Allowing 130s for 25.0ml of remaining 775.0ml @16:21:41.63 Intake.select :open @16:21:43.79 Intake @15.4psia, vacuum from 9.9 to 12.9psi @16:21:43.81 SS.reconfigure :inhaling 1.13ml/s @16:21:43.86 SS.seek 25ml @16:21:59.45 recoverPSRreagent08 @16:21:59.47 PTV.dial :puck @16:21:59.65 PBV.dial :waste1 @16:22:00.03 delay 30 seconds @16:22:08.55 Stroke completed -- waiting for pressure to equalize ... @16:22:08.56 SS.reconfigure :exhale @16:22:08.61 SS.hold @16:22:25.44 Intake.select :close @16:22:25.59 SSV.dial :exhaust @16:22:26.36 Exhaust.select :open @16:22:26.49 SS.seek 0ml @16:22:30.04 PBV.dial between PSV and waste1 @16:22:30.42 PSR.select :air @16:22:30.44 PSV.dial PSR @16:22:31.20 PS.reconfigure :fast @16:22:31.25 PS.seek 2.5ml @16:22:35.33 Exhaust.select :close @16:22:35.45 SSV.dial CBV @16:22:35.48 CBV.dial SSV @16:22:36.25 Allowing 130s for 25.0ml of remaining 750.0ml @16:22:36.27 Intake.select :open @16:22:38.44 Intake @15.3psia, vacuum from 9.8 to 12.8psi @16:22:38.46 SS.reconfigure :inhaling 1.13ml/s @16:22:38.51 SS.seek 25ml @16:22:55.79 PSV.dial PTV @16:22:56.59 delay 5 seconds @16:22:57.43 Vacuum too high -- waiting up to 40 seconds @16:22:57.45 SS.reconfigure :exhale @16:22:57.50 SS.hold @16:23:01.67 PSV.dial PSR @16:23:02.46 delay 5 seconds @16:23:04.14 Intake @14.5psia, vacuum from 9.0 to 12.0psi @16:23:04.16 SS.reconfigure :inhaling 0.91ml/s @16:23:04.21 SS.seek 25ml @16:23:07.48 PSV.dial PTV @16:23:08.24 delay 5 seconds @16:23:12.05 Stroke completed -- waiting for pressure to equalize ... @16:23:12.06 SS.reconfigure :exhale @16:23:12.11 SS.hold @16:23:13.36 PSV.dial :air @16:23:14.16 PS.seek 0ml @16:23:23.32 Intake.select :close @16:23:23.48 SSV.dial :exhaust @16:23:24.25 Exhaust.select :open @16:23:24.37 SS.seek 0ml @16:23:33.30 Exhaust.select :close @16:23:33.41 SSV.dial CBV @16:23:33.45 CBV.dial SSV @16:23:34.21 Allowing 130s for 25.0ml of remaining 725.0ml @16:23:34.22 Intake.select :open @16:23:36.39 Intake @15.3psia, vacuum from 9.8 to 12.8psi @16:23:36.41 SS.reconfigure :inhaling 0.91ml/s @16:23:36.91 SS.seek 25ml @16:23:37.98 washCycleDA #2 of 2 @16:23:37.99 PSR.select :air @16:23:38.01 PSV.dial PSR @16:23:39.34 Pulling 0.5ml of PSR.wash @16:23:39.35 PSR.select :wash @16:23:39.47 PS.reconfigure :default @16:23:39.52 PS.seek 0.5ml @16:23:45.57 delay 5 seconds @16:23:50.59 PSR.select :air @16:23:50.71 PS.seek 2ml @16:24:06.97 Stroke completed -- waiting for pressure to equalize ... @16:24:06.98 SS.reconfigure :exhale @16:24:07.03 SS.hold @16:24:07.09 PTV.dial :puck @16:24:07.21 PSV.dial PTV @16:24:07.98 PBV.dial :waste1 @16:24:08.43 PS.seek 1.5ml @16:24:14.41 delay 3 seconds @16:24:17.43 PBV.dial between PSV and waste1 @16:24:17.80 PS.seek 0ml @16:24:25.47 Intake.select :close @16:24:25.63 SSV.dial :exhaust @16:24:26.41 Exhaust.select :open @16:24:26.52 SS.seek 0ml @16:24:34.13 delay 2:00 @16:24:35.48 Exhaust.select :close @16:24:35.60 SSV.dial CBV @16:24:35.63 CBV.dial SSV @16:24:36.41 Allowing 130s for 25.0ml of remaining 700.0ml @16:24:36.42 Intake.select :open @16:24:38.59 Intake @15.2psia, vacuum from 9.7 to 12.7psi @16:24:38.61 SS.reconfigure :inhaling 0.91ml/s @16:24:38.66 SS.seek 25ml @16:25:02.71 Vacuum too high -- waiting up to 40 seconds @16:25:02.73 SS.reconfigure :exhale @16:25:02.78 SS.hold @16:25:12.82 Intake @14.9psia, vacuum from 9.4 to 12.4psi @16:25:12.84 SS.reconfigure :inhaling 0.73ml/s @16:25:12.89 SS.seek 25ml @16:25:20.08 Stroke completed -- waiting for pressure to equalize ... @16:25:20.09 SS.reconfigure :exhale @16:25:20.14 SS.hold @16:25:31.33 Intake.select :close @16:25:31.49 SSV.dial :exhaust @16:25:32.27 Exhaust.select :open @16:25:32.38 SS.seek 0ml @16:25:41.15 Exhaust.select :close @16:25:41.27 SSV.dial CBV @16:25:41.31 CBV.dial SSV @16:25:42.06 Allowing 130s for 25.0ml of remaining 675.0ml @16:25:42.08 Intake.select :open @16:25:44.24 Intake @15.3psia, vacuum from 9.8 to 12.8psi @16:25:44.69 SS.reconfigure :inhaling 0.73ml/s @16:25:44.77 SS.seek 25ml @16:26:21.23 Stroke completed -- waiting for pressure to equalize ... @16:26:21.24 SS.reconfigure :exhale @16:26:21.33 Ignoring excess vacuum after stroke's end ... @16:26:21.34 SS.reconfigure :exhale @16:26:21.40 SS.hold @16:26:34.17 recoverPSRreagent08 @16:26:34.18 PTV.dial :puck @16:26:34.25 PBV.dial :waste1 @16:26:34.62 delay 30 seconds @16:26:41.89 Intake.select :close @16:26:42.44 SSV.dial :exhaust @16:26:43.28 Exhaust.select :open @16:26:43.40 SS.seek 0ml @16:26:52.39 Exhaust.select :close @16:26:52.50 SSV.dial CBV @16:26:52.53 CBV.dial SSV @16:26:53.30 Allowing 130s for 25.0ml of remaining 650.0ml @16:26:53.31 Intake.select :open @16:26:55.47 Intake @15.1psia, vacuum from 9.6 to 12.6psi @16:26:55.49 SS.reconfigure :inhaling 0.73ml/s @16:26:55.54 SS.seek 25ml @16:27:04.64 PBV.dial between PSV and waste1 @16:27:05.00 PSR.select :air @16:27:05.02 PSV.dial PSR @16:27:05.79 PS.reconfigure :fast @16:27:05.84 PS.seek 2.5ml @16:27:23.83 Vacuum too high -- waiting up to 40 seconds @16:27:23.85 SS.reconfigure :exhale @16:27:23.91 SS.hold @16:27:30.06 PSV.dial PTV @16:27:30.83 delay 5 seconds @16:27:35.66 SS.reconfigure :inhaling 0.59ml/s @16:27:35.71 SS.seek 25ml @16:27:35.85 PSV.dial PSR @16:27:36.61 delay 5 seconds @16:27:41.63 PSV.dial PTV @16:27:42.40 delay 5 seconds @16:27:45.42 Stroke completed -- waiting for pressure to equalize ... @16:27:45.43 SS.reconfigure :exhale @16:27:45.48 SS.hold @16:27:47.41 PSV.dial :air @16:27:48.18 PS.seek 0ml @16:28:00.78 Intake.select :close @16:28:00.95 SSV.dial :exhaust @16:28:01.71 Exhaust.select :open @16:28:02.29 SS.seek 0ml @16:28:11.07 Exhaust.select :close @16:28:11.19 SSV.dial CBV @16:28:11.22 CBV.dial SSV @16:28:11.78 getPMLReagent08 @16:28:11.80 PRV.dial :air @16:28:12.00 Allowing 130s for 25.0ml of remaining 625.0ml @16:28:12.01 Intake.select :open @16:28:12.27 delay 5 seconds @16:28:14.18 Intake @15.2psia, vacuum from 9.7 to 12.7psi @16:28:14.20 SS.reconfigure :inhaling 0.59ml/s @16:28:14.25 SS.seek 25ml @16:28:17.29 PTV.dial :PRVmixing @16:28:18.60 delay 5 seconds @16:28:23.62 PRV.dial :ab2 @16:28:24.08 PTV.dial :PRVmixing @16:28:24.09 PSV.dial :mixloop @16:28:24.57 PS.reconfigure :default @16:28:24.62 PS.seek 0.3ml @16:28:28.53 delay 2 seconds @16:28:30.55 PRV.dial :air @16:28:31.10 PS.seek 0.55ml @16:28:34.53 addPMLReagent08 @16:28:34.55 PSV.dial :air @16:28:35.02 PS.seek 2ml @16:28:50.72 PSV.dial :PML @16:28:51.19 PTV.dial :mixing @16:28:51.95 PBV.dial :waste1 @16:28:52.39 PS.seek 1.85ml @16:28:54.72 PBV.dial between PSV and waste1 @16:28:55.10 PS.seek 0ml @16:28:55.20 Vacuum too high -- waiting up to 40 seconds @16:28:55.22 SS.reconfigure :exhale @16:28:55.28 SS.hold @16:29:06.59 Intake @15.1psia, vacuum from 9.6 to 12.6psi @16:29:06.61 SS.reconfigure :inhaling 0.47ml/s @16:29:06.66 SS.seek 25ml @16:29:11.95 Stroke completed -- waiting for pressure to equalize ... @16:29:11.97 SS.reconfigure :exhale @16:29:12.01 SS.hold @16:29:15.03 delay 2 seconds @16:29:17.05 delay 8:00 @16:29:26.38 Intake.select :close @16:29:26.54 SSV.dial :exhaust @16:29:27.31 Exhaust.select :open @16:29:27.42 SS.seek 0ml @16:29:36.46 Exhaust.select :close @16:29:36.58 SSV.dial CBV @16:29:36.61 CBV.dial SSV @16:29:37.39 Allowing 130s for 25.0ml of remaining 600.0ml @16:29:37.40 Intake.select :open @16:29:39.56 Intake @15.1psia, vacuum from 9.6 to 12.6psi @16:29:39.58 SS.reconfigure :inhaling 0.47ml/s @16:29:39.63 SS.seek 25ml @16:30:34.76 Stroke completed -- waiting for pressure to equalize ... @16:30:34.77 SS.reconfigure :exhale @16:30:34.82 SS.hold @16:30:59.37 Intake.select :close @16:30:59.53 SSV.dial :exhaust @16:31:00.30 Exhaust.select :open @16:31:00.42 SS.seek 0ml @16:31:09.41 Exhaust.select :close @16:31:09.53 SSV.dial CBV @16:31:09.57 CBV.dial SSV @16:31:10.32 Allowing 130s for 25.0ml of remaining 575.0ml @16:31:10.34 Intake.select :open @16:31:12.51 Intake @15.1psia, vacuum from 9.6 to 12.6psi @16:31:12.53 SS.reconfigure :inhaling 0.47ml/s @16:31:12.58 SS.seek 25ml @16:32:07.77 Stroke completed -- waiting for pressure to equalize ... @16:32:07.78 SS.reconfigure :exhale @16:32:07.83 SS.hold @16:32:38.52 Intake.select :close @16:32:38.68 SSV.dial :exhaust @16:32:39.44 Exhaust.select :open @16:32:39.56 SS.seek 0ml @16:32:48.43 Exhaust.select :close @16:32:48.54 SSV.dial CBV @16:32:48.58 CBV.dial SSV @16:32:49.33 Allowing 130s for 25.0ml of remaining 550.0ml @16:32:49.35 Intake.select :open @16:32:51.52 Intake @15.1psia, vacuum from 9.6 to 12.6psi @16:32:51.54 SS.reconfigure :inhaling 0.47ml/s @16:32:51.59 SS.seek 25ml @16:33:36.51 Vacuum too high -- waiting up to 40 seconds @16:33:36.53 SS.reconfigure :exhale @16:33:36.58 SS.hold @16:33:53.50 Intake @15.6psia, vacuum from 10.0 to 13.0psi @16:33:53.52 SS.reconfigure :inhaling 0.38ml/s @16:33:53.57 SS.seek 25ml @16:34:07.03 Stroke completed -- waiting for pressure to equalize ... @16:34:07.05 SS.reconfigure :exhale @16:34:07.09 SS.hold @16:34:29.62 Intake.select :close @16:34:29.78 SSV.dial :exhaust @16:34:30.54 Exhaust.select :open @16:34:30.65 SS.seek 0ml @16:34:39.67 Exhaust.select :close @16:34:39.79 SSV.dial CBV @16:34:39.83 CBV.dial SSV @16:34:40.57 Allowing 130s for 25.0ml of remaining 525.0ml @16:34:40.58 Intake.select :open @16:34:42.74 Intake @15.1psia, vacuum from 9.6 to 12.6psi @16:34:42.76 SS.reconfigure :inhaling 0.38ml/s @16:34:42.81 SS.seek 25ml @16:35:51.19 Stroke completed -- waiting for pressure to equalize ... @16:35:51.20 SS.reconfigure :exhale @16:35:51.25 SS.hold @16:36:26.48 Intake.select :close @16:36:26.63 SSV.dial :exhaust @16:36:27.79 Exhaust.select :open @16:36:27.97 SS.seek 0ml @16:36:36.84 Exhaust.select :close @16:36:36.96 SSV.dial CBV @16:36:36.99 CBV.dial SSV @16:36:37.78 Allowing 130s for 25.0ml of remaining 500.0ml @16:36:37.80 Intake.select :open @16:36:39.96 Intake @15.2psia, vacuum from 9.7 to 12.7psi @16:36:39.98 SS.reconfigure :inhaling 0.38ml/s @16:36:40.03 SS.seek 25ml @16:37:17.07 recoverPRVreagent08 @16:37:17.08 PTV.dial :mixing @16:37:17.16 PBV.dial :waste1 @16:37:17.54 delay 30 seconds @16:37:40.51 Vacuum too high -- waiting up to 40 seconds @16:37:40.52 SS.reconfigure :exhale @16:37:40.58 SS.hold @16:37:47.56 PBV.dial between PSV and waste1 @16:37:47.96 PSV.dial :air @16:37:48.60 PS.reconfigure :fast @16:37:48.65 PS.seek 2.5ml @16:38:03.12 Intake @15.6psia, vacuum from 10.0 to 13.0psi @16:38:03.14 SS.reconfigure :inhaling 0.31ml/s @16:38:03.19 SS.seek 25ml @16:38:12.96 PSV.dial :mixloop @16:38:13.41 delay 2 seconds @16:38:14.17 Stroke completed -- waiting for pressure to equalize ... @16:38:14.18 SS.reconfigure :exhale @16:38:14.23 SS.hold @16:38:15.50 PSV.dial :air @16:38:15.96 delay 2 seconds @16:38:17.98 PSV.dial :mixloop @16:38:18.46 delay 2 seconds @16:38:20.47 PSV.dial :air @16:38:20.95 PS.seek 0ml @16:38:38.78 Intake.select :close @16:38:38.93 SSV.dial :exhaust @16:38:39.71 Exhaust.select :open @16:38:39.82 SS.seek 0ml @16:38:44.59 washCycleDA #1 of 3 @16:38:44.60 PSR.select :air @16:38:44.61 PSV.dial PSR @16:38:45.96 Pulling 0.5ml of PSR.wash @16:38:45.97 PSR.select :wash @16:38:46.09 PS.reconfigure :default @16:38:46.14 PS.seek 0.5ml @16:38:48.67 Exhaust.select :close @16:38:48.78 SSV.dial CBV @16:38:48.82 CBV.dial SSV @16:38:49.58 Allowing 130s for 25.0ml of remaining 475.0ml @16:38:49.59 Intake.select :open @16:38:51.76 Intake @15.0psia, vacuum from 9.5 to 12.5psi @16:38:51.78 SS.reconfigure :inhaling 0.31ml/s @16:38:51.83 SS.seek 25ml @16:38:52.15 delay 5 seconds @16:38:57.16 PSR.select :air @16:38:57.28 PS.seek 2ml @16:39:13.49 PTV.dial :puck @16:39:14.24 PSV.dial PTV @16:39:14.99 PBV.dial :waste1 @16:39:15.43 PS.seek 1.5ml @16:39:21.43 delay 3 seconds @16:39:24.45 PBV.dial between PSV and waste1 @16:39:24.82 PS.seek 0ml @16:39:40.99 delay 2:00 @16:40:21.04 Stroke completed -- waiting for pressure to equalize ... @16:40:21.06 SS.reconfigure :exhale @16:40:21.10 SS.hold @16:40:59.60 SS.reconfigure :default @16:40:59.66 SS.hold @16:40:59.73 Intake.select :close @16:40:59.89 ShallowSampler::Clogged in WCR -- Filtering too Slowly after 550.0ml @16:40:59.91 SSV.dial :exhaust @16:41:00.67 Exhaust.select :open @16:41:00.79 SS.reconfigure :exhale @16:41:00.84 SS.seek 0ml @16:41:09.65 Exhaust.select :close @16:41:09.77 Sampled 550.0ml @16:41:09.79 Drying puck before fixing for 5:00 @16:41:09.80 CBV.dial :waste2b @16:41:10.57 delay 1:00 @16:41:41.01 recoverPSRreagent08 @16:41:41.02 PTV.dial :puck @16:41:41.10 PBV.dial :waste1 @16:41:41.47 delay 30 seconds @16:42:10.60 CSV.dial :air @16:42:11.06 CS.reconfigure :fast @16:42:11.11 CS.seek 10ml @16:42:11.49 PBV.dial between PSV and waste1 @16:42:11.86 PSR.select :air @16:42:11.88 PSV.dial PSR @16:42:12.65 PS.reconfigure :fast @16:42:12.70 PS.seek 2.5ml @16:42:35.96 Pumping wcr puck from tube 2 up to 35psi... @16:42:35.98 CBV.dial SSV @16:42:36.83 PSV.dial PTV @16:42:37.25 CBV.dial between CSV and waste2b @16:42:37.60 delay 5 seconds @16:42:38.20 CSV.dial between air and CTV @16:42:38.53 CS.seek 7.304ml @16:42:42.61 PSV.dial PSR @16:42:43.38 delay 5 seconds @16:42:45.62 CTV.dial CSV,avoiding: :air @16:42:45.65 CSV.dial CTV @16:42:46.45 CS.reconfigure :pressurize @16:42:46.50 CS.seek 0ml @16:42:48.40 PSV.dial PTV @16:42:49.16 delay 5 seconds @16:42:54.18 PSV.dial :air @16:42:54.94 PS.seek 0ml @16:43:05.71 CSV.dial :air @16:43:06.17 CS.reconfigure :fast @16:43:06.22 CS.seek 10ml @16:43:18.46 washCycleDA #2 of 3 @16:43:18.47 PSR.select :air @16:43:18.48 PSV.dial PSR @16:43:19.81 Pulling 0.5ml of PSR.wash @16:43:19.82 PSR.select :wash @16:43:19.94 PS.reconfigure :default @16:43:19.99 PS.seek 0.5ml @16:43:25.89 delay 5 seconds @16:43:30.91 PSR.select :air @16:43:31.03 PS.seek 2ml @16:43:31.26 CSV.dial between air and CTV @16:43:31.61 CS.seek 4.019ml @16:43:46.30 CTV.dial CSV,avoiding: :air @16:43:46.34 CSV.dial CTV @16:43:46.68 CS.reconfigure :pressurize @16:43:46.73 CS.seek 0ml @16:43:47.23 PTV.dial :puck @16:43:47.31 PSV.dial PTV @16:43:48.06 PBV.dial :waste1 @16:43:48.50 PS.seek 1.5ml @16:43:54.39 delay 3 seconds @16:43:57.41 PBV.dial between PSV and waste1 @16:43:57.61 CSV.dial :air @16:43:57.77 PS.seek 0ml @16:43:58.07 CS.reconfigure :fast @16:43:58.13 CS.seek 10ml @16:44:13.95 delay 2:00 @16:44:22.64 CSV.dial between air and CTV @16:44:22.96 CS.seek 4.139ml @16:44:37.27 CTV.dial CSV,avoiding: :air @16:44:37.30 CSV.dial CTV @16:44:37.62 CS.reconfigure :pressurize @16:44:37.67 CS.seek 0ml @16:44:48.80 CSV.dial :air @16:44:49.26 CS.reconfigure :fast @16:44:49.31 CS.seek 10ml @16:45:13.89 CSV.dial between air and CTV @16:45:14.21 CS.seek 4.15ml @16:45:28.93 CTV.dial CSV,avoiding: :air @16:45:28.96 CSV.dial CTV @16:45:29.40 CS.reconfigure :pressurize @16:45:29.46 CS.seek 0ml @16:45:40.58 CSV.dial :air @16:45:41.02 CS.reconfigure :default @16:45:41.07 CS.hold @16:45:41.21 ShallowSampler::Error in MAIN -- Puck.from 2, :wcr reached only 21.6 of 35psi after pumping in 40ml of gas @16:45:41.22 Waiting to Resume from Checkpoint "Pumping wcr puck from tube 2 up to 35psi..." @16:45:41.24 BadNews.email "Puck.from 2, :wcr reached only 21.6 of 35psi after pumping in 40ml of gas",Subject: "ShallowSampler::Error in MAIN" @16:45:45.50 upsync @16:46:13.98 recoverPSRreagent08 @16:46:14.00 PTV.dial :puck @16:46:14.08 PBV.dial :waste1 @16:46:14.44 delay 30 seconds @16:46:44.47 PBV.dial between PSV and waste1 @16:46:44.83 PSR.select :air @16:46:44.85 PSV.dial PSR @16:46:45.61 PS.reconfigure :fast @16:46:45.67 PS.seek 2.5ml @16:47:09.70 PSV.dial PTV @16:47:10.47 delay 5 seconds @16:47:15.49 PSV.dial PSR @16:47:16.25 delay 5 seconds @16:47:21.27 PSV.dial PTV @16:47:22.03 delay 5 seconds @16:47:27.05 PSV.dial :air @16:47:27.93 PS.seek 0ml @16:47:51.45 washCycleDA #3 of 3 @16:47:51.46 PSR.select :air @16:47:51.48 PSV.dial PSR @16:47:52.93 Pulling 0.5ml of PSR.wash @16:47:52.94 PSR.select :wash @16:47:53.06 PS.reconfigure :default @16:47:53.12 PS.seek 0.5ml @16:47:59.14 delay 5 seconds @16:48:04.16 PSR.select :air @16:48:04.28 PS.seek 2ml @16:48:20.48 PTV.dial :puck @16:48:20.56 PSV.dial PTV @16:48:21.31 PBV.dial :waste1 @16:48:21.73 PS.seek 1.5ml @16:48:27.76 delay 3 seconds @16:48:30.78 PBV.dial between PSV and waste1 @16:48:31.14 PS.seek 0ml @16:48:47.29 delay 2:00 @16:50:47.32 recoverPSRreagent08 @16:50:47.33 PTV.dial :puck @16:50:47.41 PBV.dial :waste1 @16:50:48.27 delay 30 seconds @16:51:18.30 PBV.dial between PSV and waste1 @16:51:18.68 PSR.select :air @16:51:18.70 PSV.dial PSR @16:51:19.47 PS.reconfigure :fast @16:51:19.52 PS.seek 2.5ml @16:51:43.60 PSV.dial PTV @16:51:44.37 delay 5 seconds @16:51:49.39 PSV.dial PSR @16:51:50.15 delay 5 seconds @16:51:55.17 PSV.dial PTV @16:51:55.93 delay 5 seconds @16:52:00.95 PSV.dial :air @16:52:01.73 PS.seek 0ml @16:52:25.04 Gate.power :camera,:ON @16:52:25.05 Mixing DA Substrates @16:52:25.06 PSR.select :air @16:52:25.08 PSV.dial PSR @16:52:26.47 Pulling 0.25ml of PSR.sub1 @16:52:26.50 PSR.select :sub1 @16:52:26.72 PS.reconfigure :default @16:52:26.84 PS.seek 0.25ml @16:52:30.30 delay 5 seconds @16:52:35.32 Pulling 0.25ml of PSR.sub2 @16:52:35.33 PSR.select :sub2 @16:52:35.45 PS.seek 0.5ml @16:52:39.01 delay 5 seconds @16:52:44.03 PSR.select :air @16:52:44.14 PS.seek 2ml @16:53:00.28 PTV.dial :puck @16:53:00.36 PSV.dial PTV @16:53:01.13 PBV.dial :waste1 @16:53:01.57 PS.seek 1.5ml @16:53:07.57 delay 3 seconds @16:53:10.59 PBV.dial between PSV and waste1 @16:53:10.96 PS.seek 0ml @16:53:27.16 delay 10 seconds @16:53:37.19 recoverPSRreagent08 @16:53:37.20 PTV.dial :puck @16:53:37.27 PBV.dial :waste1 @16:53:37.65 delay 30 seconds @16:54:07.67 PBV.dial between PSV and waste1 @16:54:08.05 PSR.select :air @16:54:08.07 PSV.dial PSR @16:54:08.85 PS.reconfigure :fast @16:54:08.90 PS.seek 2.5ml @16:54:33.28 PSV.dial PTV @16:54:34.07 delay 5 seconds @16:54:39.09 PSV.dial PSR @16:54:39.85 delay 5 seconds @16:54:44.87 PSV.dial PTV @16:54:45.77 delay 5 seconds @16:54:50.79 PSV.dial :air @16:54:51.70 PS.seek 0ml @16:55:15.82 Additional air clear @16:55:15.83 PSR.select :air @16:55:15.84 PSV.dial PSR @16:55:17.23 Pulling 2ml of PSR.air @16:55:17.25 PSR.select :air @16:55:17.29 PS.reconfigure :default @16:55:17.34 PS.seek 2ml @16:55:38.53 delay 5 seconds @16:55:43.55 PTV.dial :puck @16:55:43.64 PSV.dial PTV @16:55:44.42 PBV.dial :waste1 @16:55:44.86 PS.seek 1.5ml @16:55:51.31 delay 3 seconds @16:55:54.33 PBV.dial between PSV and waste1 @16:55:54.71 delay 1 second @16:55:55.73 PSV.dial :waste1 @16:55:56.81 PS.seek 0ml @16:56:13.05 Processing Puck Heater at 27.9C before imaging @16:56:13.07 PH.coast @16:56:13.19 Imaging da puck in Processing Clamp while storing it to tube 1 @16:56:13.36 SC.reconfigure :tight @16:56:13.42 SC.seek 1 @16:56:17.02 SC.configure :limp @16:56:17.24 Elbow.reconfigure :tight @16:56:17.29 Elbow.seek 1 @16:56:19.03 Forearm.reconfigure :tight @16:56:19.08 Forearm.seek 1 + 450 counts @16:56:20.11 SE.reconfigure :pick @16:56:20.16 SE.seek :beamBreak @16:56:21.39 SE.configure :default @16:56:21.55 Elbow.reconfigure :limp @16:56:21.60 Forearm.reconfigure :loose @16:56:21.65 Forearm.seek 1 @16:56:21.87 Elbow.hold @16:56:21.94 Elbow.reconfigure :tight @16:56:22.04 SE.seek 29.03 Pucks below Top Plate @16:56:22.52 Forearm.force -20 @16:56:22.57 Forearm.seek 1 + 314 counts @16:56:22.87 Forearm.reconfigure :tight @16:56:22.92 Forearm.seek 1 + 450 counts @16:56:23.14 SE.seek 30.03 Pucks below Top Plate @16:56:23.71 Forearm.reconfigure :loose @16:56:23.76 Forearm.seek :retract @16:56:24.73 Elbow.seek PC @16:56:27.44 Elbow.reconfigure :loose @16:56:27.55 Forearm.seek PC @16:56:29.00 Hand.close @16:56:29.64 PC.seek :open @16:56:34.46 Forearm.seek :retract @16:56:35.47 Elbow.reconfigure :tight @16:56:35.52 Elbow.seek 1 @16:56:38.18 Forearm.reconfigure :tight @16:56:38.23 Forearm.seek 1 + 450 counts @16:56:39.29 Hand.open @16:56:39.95 SE.seek 29.68 Pucks below Top Plate @16:56:40.21 SE.reconfigure :seal @16:56:40.26 SE.seek 28.72 Pucks below Top Plate @16:56:40.78 Lights, camera, ACTION! @16:56:40.81 PuckCamera: snap -bin=2,2 -auto /var/log/esp/da23oct1216h650ml.tif @16:57:00.32 Waiting >20 seconds for da to relinquish FlushPuck @16:57:03.47 SX HX9/16 (USB:08): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 19.374 seconds /var/log/esp/da23oct1216h650ml.tif: TIFF Upload Complete @16:57:03.50 PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da23oct1216h650ml15s.tif @16:57:19.15 SX HX9/16 (USB:08): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15 seconds /var/log/esp/midres/da23oct1216h650ml15s.tif: TIFF Upload Complete @16:57:19.18 PuckCamera: snap -bin=2,2 20.0 /var/log/esp/midres/da23oct1216h650ml20s.tif @16:57:39.79 SX HX9/16 (USB:08): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 20 seconds /var/log/esp/midres/da23oct1216h650ml20s.tif: TIFF Upload Complete @16:57:39.81 Gate.power :camera,:OFF @16:57:39.83 DAfans.email "19.374 sec auto-exposure",Subject: "da23oct1216h650ml images!" @16:57:40.27 Elbow.seek 1 @16:57:40.83 Forearm.seek 1 + 450 counts @16:57:41.04 SE.reconfigure :gently @16:57:41.09 SE.seek 29.43 Pucks below Top Plate @16:57:41.80 Elbow.reconfigure :limp @16:57:41.88 Forearm.reconfigure :loose @16:57:41.93 Forearm.seek 1 @16:57:42.23 Elbow.hold @16:57:42.30 Elbow.reconfigure :tight @16:57:42.36 SE.reconfigure :default @16:57:42.41 SE.seek 29.62 Pucks below Top Plate @16:57:43.09 Forearm.force -20 @16:57:43.19 Forearm.seek 1 + 315 counts @16:57:44.54 Forearm.reconfigure :tight @16:57:44.66 Forearm.seek 1 + 450 counts @16:57:45.11 SE.seek :down @16:57:45.87 SE.stop @16:57:46.43 Forearm.reconfigure :loose @16:57:46.53 Forearm.seek :retract @16:57:47.77 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @16:57:48.14 Elbow.seek FlushPuck::Garage @16:57:50.10 Elbow.reconfigure :loose @16:57:50.36 Forearm.seek :garage @16:57:51.39 Hand.close @16:57:52.26 Forearm.seek :retract @16:57:53.30 Elbow.reconfigure :tight @16:57:53.40 Elbow.seek PC @16:57:54.76 Elbow.reconfigure :loose @16:57:54.97 Forearm.reconfigure :tight @16:57:55.06 Forearm.seek PC @16:57:56.62 PC.seek :closed @16:58:01.40 Processing Clamp onSpring/offSpring for 16/26 samples, on/off torque 1.62 @16:58:01.54 Hand.open @16:58:02.29 Forearm.reconfigure :loose @16:58:02.38 Forearm.seek :retract @16:58:03.34 PSR.select :air @16:58:03.38 PSV.dial PSR @16:58:03.90 Pulling 0.5ml of PSR.flush @16:58:03.92 PSR.select :flush @16:58:04.09 PS.seek 0.5ml @16:58:10.06 delay 5 seconds @16:58:14.05 upsync @16:58:16.02 PSR.select :air @16:58:16.15 PS.seek 2.5ml @16:58:37.47 delay 1 second @16:58:38.49 PRV.dial :waste2,avoiding: :ab2 @16:58:40.36 PTV.dial :PRVmixing @16:58:41.69 PSV.dial :mixloop @16:58:42.73 PS.seek :empty @16:59:09.06 PSR.select :air @16:59:09.08 PSV.dial PSR @16:59:10.14 Pulling 0.5ml of PSR.flush @16:59:10.15 PSR.select :flush @16:59:10.27 PS.seek 0.5ml @16:59:16.28 delay 5 seconds @16:59:21.30 PSR.select :air @16:59:21.42 PS.seek 2.5ml @16:59:42.68 delay 1 second @16:59:43.70 PBV.dial :waste1 @16:59:44.07 PTV.dial :mixing @16:59:44.82 PSV.dial :mixloop @16:59:45.89 PS.seek :empty @17:00:12.31 PSV.dial PSR @17:00:13.35 PSR.select :flush @17:00:13.48 PS.seek 1ml @17:00:24.49 PSR.select :air @17:00:24.61 PS.seek 2.5ml @17:00:40.71 PSV.dial PTV @17:00:41.48 PTV.dial :puck @17:00:42.21 PBV.dial :waste1 @17:00:42.30 PS.seek 0ml @17:01:08.62 PSV.dial PSR @17:01:09.39 PSR.select :flush @17:01:09.51 PS.seek 0.5ml @17:01:15.46 PSR.select :air @17:01:15.58 PS.seek 2.5ml @17:01:36.81 PSV.dial PTV @17:01:37.57 PBV.dial PSV @17:01:38.27 PS.seek 0ml @17:02:04.65 PSV.dial PBV @17:02:05.12 PS.seek 2.5ml @17:02:31.49 PSV.dial :waste1 @17:02:32.23 PS.seek 0ml @17:02:58.62 PSV.dial PSR @17:02:59.09 PSR.select :air @17:02:59.11 PS.seek 2.5ml @17:03:25.52 PSV.dial PTV @17:03:26.26 PBV.dial PSV @17:03:26.34 PS.seek 0ml @17:03:52.74 PSV.dial PBV @17:03:53.22 PS.seek 2.5ml @17:04:19.62 PSV.dial :waste1 @17:04:20.40 PS.seek 0ml @17:04:46.71 PSV.dial PSR @17:04:47.18 PSR.select :flush @17:04:47.30 PS.seek 1ml @17:04:58.30 PSR.select :air @17:04:58.42 PS.seek 2.5ml @17:05:14.55 PSV.dial PTV @17:05:15.29 PTV.dial :puck @17:05:15.30 PBV.dial :waste1 @17:05:15.86 PS.seek 0ml @17:05:42.15 PSV.dial PSR @17:05:42.91 PSR.select :flush @17:05:43.03 PS.seek 0.5ml @17:05:48.96 PSR.select :air @17:05:49.08 PS.seek 2.5ml @17:06:10.52 PSV.dial PTV @17:06:11.29 PBV.dial PSV @17:06:11.85 PS.seek 0ml @17:06:38.42 PSV.dial PBV @17:06:38.90 PS.seek 2.5ml @17:07:05.21 PSV.dial :waste1 @17:07:05.95 PS.seek 0ml @17:07:32.33 PSV.dial PSR @17:07:32.80 PSR.select :air @17:07:32.83 PS.seek 2.5ml @17:07:59.24 PSV.dial PTV @17:08:00.10 PBV.dial PSV @17:08:00.29 PS.seek 0ml @17:08:26.61 PSV.dial PBV @17:08:27.07 PS.seek 2.5ml @17:08:53.42 PSV.dial :waste1 @17:08:54.29 PS.seek 0ml @17:09:20.67 Garaging FlushPuck in Processing Clamp @17:09:20.75 Elbow.reconfigure :tight @17:09:20.80 Elbow.seek PC @17:09:20.92 Elbow.reconfigure :loose @17:09:21.02 Forearm.seek PC @17:09:21.99 Hand.close @17:09:22.63 PC.seek :open @17:09:27.83 Forearm.seek :retract @17:09:28.80 Elbow.reconfigure :tight @17:09:28.85 Elbow.seek :garage @17:09:30.20 Elbow.reconfigure :loose @17:09:30.31 Forearm.reconfigure :tight @17:09:30.36 Forearm.seek :garage @17:09:31.31 Hand.open @17:09:31.94 Forearm.reconfigure :loose @17:09:31.99 Forearm.seek :retract @18:19:28.39 Session Begun @18:19:33.38 -> Flush FlushPuck is in FlushPuck::Garage @18:19:36.59 -> CC Collection Clamp contains wcr puck from tube 2 @18:19:41.19 -> PC Processing Clamp open @18:19:51.49 -> Puck.count @18:19:51.58 SE.seek :down @18:19:51.69 SE.stop @18:19:51.81 SC.reconfigure :tight @18:19:51.88 SC.seek 1 @18:19:52.00 SC.configure :limp @18:19:52.25 Elbow.reconfigure :tight @18:19:52.32 Elbow.seek 1 @18:19:54.20 Forearm.reconfigure :tight @18:19:54.27 Forearm.seek 1 + 450 counts @18:19:55.35 SE.reconfigure :place @18:19:55.43 SE.seek :beamBreak @18:19:56.79 SE.configure :default @18:19:56.92 SE.seek :down @18:19:57.53 SE.stop @18:19:58.06 Forearm.reconfigure :loose @18:19:58.13 Forearm.seek :retract @18:19:59.04 SC.reconfigure :tight @18:19:59.12 SC.seek 2 @18:20:02.68 SC.configure :limp @18:20:02.95 Elbow.seek 2 @18:20:03.36 Forearm.reconfigure :tight @18:20:03.44 Forearm.seek 2 + 450 counts @18:20:04.52 SE.reconfigure :place @18:20:04.99 SE.seek :beamBreak @18:20:08.34 SE.configure :default @18:20:08.47 SE.seek :down @18:20:09.61 Forearm.reconfigure :loose @18:20:09.68 Forearm.seek :retract @18:20:11.16 SE.stop @18:20:11.22 SC.reconfigure :tight @18:20:11.29 SC.seek 3 @18:20:14.77 SC.configure :limp @18:20:14.95 Elbow.seek 3 @18:20:15.30 Forearm.reconfigure :tight @18:20:15.38 Forearm.seek 3 + 450 counts @18:20:16.37 SE.reconfigure :place @18:20:16.45 SE.seek :beamBreak @18:20:17.58 SE.configure :default @18:20:17.72 SE.seek :down @18:20:18.14 SE.stop @18:20:18.86 Forearm.reconfigure :loose @18:20:18.93 Forearm.seek :retract @18:20:19.91 SC.reconfigure :tight @18:20:19.98 SC.seek 4 @18:20:23.57 SC.configure :limp @18:20:23.74 Elbow.seek 4 @18:20:24.46 Forearm.reconfigure :tight @18:20:24.53 Forearm.seek 4 + 450 counts @18:20:25.63 SE.reconfigure :place @18:20:25.71 SE.seek :beamBreak @18:20:26.85 SE.configure :default @18:20:26.98 SE.seek :down @18:20:27.41 SE.stop @18:20:28.11 Forearm.reconfigure :loose @18:20:28.19 Forearm.seek :retract @18:20:29.11 SC.reconfigure :tight @18:20:29.18 SC.seek 5 @18:20:32.71 SC.configure :limp @18:20:32.88 Elbow.seek 5 @18:20:33.55 Forearm.reconfigure :tight @18:20:33.62 Forearm.seek 5 + 450 counts @18:20:34.68 SE.reconfigure :place @18:20:34.76 SE.seek :beamBreak @18:20:35.95 SE.configure :default @18:20:36.08 SE.seek :down @18:20:36.56 SE.stop @18:20:37.21 Forearm.reconfigure :loose @18:20:37.29 Forearm.seek :retract @18:20:38.31 SC.reconfigure :tight @18:20:38.38 SC.seek 6 @18:20:41.96 SC.configure :limp @18:20:42.13 Elbow.seek 6 @18:20:42.35 Forearm.reconfigure :tight @18:20:42.42 Forearm.seek 6 + 450 counts @18:20:43.59 SE.reconfigure :place @18:20:43.68 SE.seek :beamBreak @18:20:44.87 SE.configure :default @18:20:44.99 SE.seek :down @18:20:45.41 SE.stop @18:20:46.13 Forearm.reconfigure :loose @18:20:46.20 Forearm.seek :retract @18:20:47.18 SC.reconfigure :tight @18:20:47.26 SC.seek 7 @18:20:50.78 SC.configure :limp @18:20:50.96 Elbow.seek 7 @18:20:51.56 Forearm.reconfigure :tight @18:20:51.64 Forearm.seek 7 + 450 counts @18:20:52.74 SE.reconfigure :place @18:20:52.82 SE.seek :beamBreak @18:20:54.13 SE.configure :default @18:20:54.26 SE.seek :down @18:20:54.92 SE.stop @18:20:55.39 Forearm.reconfigure :loose @18:20:55.46 Forearm.seek :retract {1 => 31, 2 => 1, 3 => 33, 4 => 33, 5 => 33, 6 => 33, 7 => 31} @18:21:49.47 -> MainThread.resume @18:21:49.49
Resuming Thread (suspended since 16:42:35) # @18:21:49.54 Pumping wcr puck from tube 2 up to 35psi... @18:21:49.96 CBV.dial SSV @18:21:51.46 CBV.dial between CSV and waste2b @18:21:52.44 CSV.dial between air and CTV @18:21:52.77 CS.reconfigure :fast @18:21:52.85 CS.seek 5.712ml @18:22:08.52 CTV.dial CSV,avoiding: :air @18:22:08.55
CSV.dial CTV @18:22:08.88 CS.reconfigure :pressurize @18:22:08.96 CS.seek 0ml @18:22:24.17 CSV.dial :air @18:22:24.65 CS.reconfigure :fast @18:22:24.72 CS.seek 10ml @18:22:49.26 CSV.dial between air and CTV @18:22:49.62 CS.seek 5.701ml @18:23:00.21 CTV.dial CSV,avoiding: :air @18:23:00.24
CSV.dial CTV @18:23:00.57 CS.reconfigure :pressurize @18:23:00.65 CS.seek 0ml @18:23:15.73 CSV.dial :air @18:23:16.21 CS.reconfigure :fast @18:23:16.28 CS.seek 10ml @18:23:40.63 CSV.dial between air and CTV @18:23:40.97 CS.seek 4.003ml @18:23:55.39 CTV.dial CSV,avoiding: :air @18:23:55.42
CSV.dial CTV @18:23:55.75 CS.reconfigure :pressurize @18:23:55.83 CS.seek 0ml @18:24:06.64 CSV.dial :air @18:24:07.16 CS.reconfigure :fast @18:24:07.24 CS.seek 10ml @18:24:31.73 CSV.dial between air and CTV @18:24:32.07 CS.seek 4.1ml @18:24:46.80 CTV.dial CSV,avoiding: :air @18:24:46.83
CSV.dial CTV @18:24:47.18 CS.reconfigure :pressurize @18:24:47.25 CS.seek 0ml @18:24:58.32 CSV.dial :air @18:24:58.81 CS.reconfigure :default @18:24:58.88 CS.hold @18:24:59.04 ShallowSampler::Error in MAIN -- Puck.from 2, :wcr reached only 21.9 of 35psi after pumping in 40ml of gas @18:24:59.06 Waiting to Resume from Checkpoint "Pumping wcr puck from tube 2 up to 35psi..." @18:24:59.09 BadNews.email "Puck.from 2, :wcr reached only 21.9 of 35psi after pumping in 40ml of gas",Subject: "ShallowSampler::Error in MAIN" @18:25:03.44 upsync @18:28:13.56 -> CC.unloadPuck 1, :WCR @18:28:13.66 Puck::Warning in dave -- Unloading Puck.from 2, :wcr to Storage Carousel @18:28:13.68 Storing WCR puck in Collection Clamp to tube 1 @18:28:13.89 SC.reconfigure :tight @18:28:13.97 SC.seek 1 @18:28:33.32 SC.configure :limp @18:28:33.52 Elbow.seek 1 @18:28:34.16 Forearm.reconfigure :tight @18:28:34.24 Forearm.seek 1 + 450 counts @18:28:35.33 SE.reconfigure :pick @18:28:35.41 SE.seek :beamBreak @18:28:36.52 SE.configure :default @18:28:36.70 Elbow.reconfigure :limp @18:28:36.78 Forearm.reconfigure :loose @18:28:36.84 Forearm.seek 1 @18:28:37.14 Elbow.hold @18:28:37.23 Elbow.reconfigure :tight @18:28:37.35 SE.seek 30.02 Pucks below Top Plate @18:28:37.82 Forearm.force -20 @18:28:37.88 Forearm.seek 1 + 313 counts @18:28:38.21 Forearm.reconfigure :tight @18:28:38.28 Forearm.seek 1 + 450 counts @18:28:38.58 SE.seek 31.02 Pucks below Top Plate @18:28:39.17 Forearm.reconfigure :loose @18:28:39.23 Forearm.seek :retract @18:28:40.68 Elbow.seek CC @18:28:42.40 Elbow.reconfigure :loose @18:28:42.52 Forearm.seek CC @18:28:43.52 Hand.close @18:28:44.19 CC.seek :open @18:28:49.00 Forearm.seek :retract @18:28:49.96 Elbow.reconfigure :tight @18:28:50.03 Elbow.seek 1 @18:28:51.80 Elbow.reconfigure :limp @18:28:51.88 Forearm.seek 1 @18:28:52.92 Elbow.hold @18:28:53.01 Elbow.reconfigure :tight @18:28:53.08 Hand.open @18:28:53.82 Forearm.force -20 @18:28:53.88 Forearm.seek 1 + 319 counts @18:28:54.26 Forearm.reconfigure :tight @18:28:54.33 Forearm.seek 1 + 450 counts @18:28:54.66 SE.seek :down @18:28:55.28 SE.stop @18:28:55.79 Forearm.reconfigure :loose @18:28:55.86 Forearm.seek :retract @18:30:43.22 -> Puck.count @18:30:43.30 SE.seek :down @18:30:43.39 SE.stop @18:30:43.50 SC.reconfigure :tight @18:30:43.57 SC.seek 1 @18:30:43.70 SC.configure :limp @18:30:43.88 Elbow.seek 1 @18:30:44.08 Forearm.reconfigure :tight @18:30:44.16 Forearm.seek 1 + 450 counts @18:30:45.20 SE.reconfigure :place @18:30:45.28 SE.seek :beamBreak @18:30:46.59 SE.configure :default @18:30:46.72 SE.seek :down @18:30:47.24 SE.stop @18:30:48.32 Forearm.reconfigure :loose @18:30:48.39 Forearm.seek :retract @18:30:49.36 SC.reconfigure :tight @18:30:49.44 SC.seek 2 @18:30:53.04 SC.configure :limp @18:30:53.22 Elbow.seek 2 @18:30:53.44 Forearm.reconfigure :tight @18:30:53.51 Forearm.seek 2 + 450 counts @18:30:54.55 SE.reconfigure :place @18:30:54.63 SE.seek :beamBreak @18:30:57.87 SE.configure :default @18:30:58.00 SE.seek :down @18:30:59.14 Forearm.reconfigure :loose @18:30:59.21 Forearm.seek :retract @18:31:00.71 SE.stop @18:31:00.78 SC.reconfigure :tight @18:31:00.85 SC.seek 3 @18:31:04.38 SC.configure :limp @18:31:04.55 Elbow.seek 3 @18:31:05.13 Forearm.reconfigure :tight @18:31:05.21 Forearm.seek 3 + 450 counts @18:31:06.25 SE.reconfigure :place @18:31:06.33 SE.seek :beamBreak @18:31:07.50 SE.configure :default @18:31:07.63 SE.seek :down @18:31:08.06 SE.stop @18:31:08.76 Forearm.reconfigure :loose @18:31:08.83 Forearm.seek :retract @18:31:09.86 SC.reconfigure :tight @18:31:09.93 SC.seek 4 @18:31:13.56 SC.configure :limp @18:31:13.74 Elbow.seek 4 @18:31:14.20 Forearm.reconfigure :tight @18:31:14.27 Forearm.seek 4 + 450 counts @18:31:15.34 SE.reconfigure :place @18:31:15.42 SE.seek :beamBreak @18:31:17.03 SE.configure :default @18:31:17.16 SE.seek :down @18:31:17.59 SE.stop @18:31:18.30 Forearm.reconfigure :loose @18:31:18.37 Forearm.seek :retract @18:31:19.35 SC.reconfigure :tight @18:31:19.42 SC.seek 5 @18:31:22.96 SC.configure :limp @18:31:23.14 Elbow.seek 5 @18:31:23.48 Forearm.reconfigure :tight @18:31:23.55 Forearm.seek 5 + 450 counts @18:31:24.59 SE.reconfigure :place @18:31:24.68 SE.seek :beamBreak @18:31:25.84 SE.configure :default @18:31:25.97 SE.seek :down @18:31:26.45 SE.stop @18:31:27.10 Forearm.reconfigure :loose @18:31:27.17 Forearm.seek :retract @18:31:28.13 SC.reconfigure :tight @18:31:28.20 SC.seek 6 @18:31:31.77 SC.configure :limp @18:31:31.95 Elbow.seek 6 @18:31:32.16 Forearm.reconfigure :tight @18:31:32.24 Forearm.seek 6 + 450 counts @18:31:33.24 SE.reconfigure :place @18:31:33.32 SE.seek :beamBreak @18:31:34.51 SE.configure :default @18:31:34.64 SE.seek :down @18:31:35.07 SE.stop @18:31:35.78 Forearm.reconfigure :loose @18:31:35.85 Forearm.seek :retract @18:31:36.80 SC.reconfigure :tight @18:31:36.87 SC.seek 7 @18:31:40.42 SC.configure :limp @18:31:40.59 Elbow.seek 7 @18:31:41.15 Forearm.reconfigure :tight @18:31:41.61 Forearm.seek 7 + 450 counts @18:31:42.83 SE.reconfigure :place @18:31:42.91 SE.seek :beamBreak @18:31:44.27 SE.configure :default @18:31:44.40 SE.seek :down @18:31:45.02 SE.stop @18:31:45.55 Forearm.reconfigure :loose @18:31:45.62 Forearm.seek :retract {1 => 32, 2 => 1, 3 => 33, 4 => 33, 5 => 33, 6 => 33, 7 => 31} @18:32:26.54 -> CS.flush @18:32:26.56 NoMethodError in dave -- undefined method `flush' for CS:Syringe @18:32:35.19 -> PS.flush @18:32:35.21 Flushing Processing stage @18:32:35.30 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @18:32:35.50 Elbow.seek FlushPuck::Garage @18:32:37.37 Elbow.reconfigure :loose @18:32:37.49 Forearm.seek :garage @18:32:38.45 Hand.close @18:32:39.18 Forearm.seek :retract @18:32:40.17 Elbow.reconfigure :tight @18:32:40.25 Elbow.seek PC @18:32:41.52 Elbow.reconfigure :loose @18:32:41.64 Forearm.reconfigure :tight @18:32:41.72 Forearm.seek PC @18:32:42.99 PC.seek :closed @18:32:47.65 Processing Clamp onSpring/offSpring for 16/26 samples, on/off torque 1.62 @18:32:47.75 Hand.open @18:32:48.50 Forearm.reconfigure :loose @18:32:48.56 Forearm.seek :retract @18:32:49.53 PSV.dial PSR @18:32:50.01 PSR.select :flush @18:32:50.16 PS.seek 1ml @18:33:01.26 PSR.select :air @18:33:01.40 PS.seek 2.5ml @18:33:17.62 PSV.dial PTV @18:33:18.37 PTV.dial :puck @18:33:18.39 PBV.dial :waste1 @18:33:18.97 PS.seek 0ml @18:33:45.39 PSV.dial PSR @18:33:46.28 PSR.select :flush @18:33:46.42 PS.seek 0.5ml @18:33:52.36 PSR.select :air @18:33:52.50 PS.seek 2.5ml @18:34:13.70 PSV.dial PTV @18:34:14.58 PBV.dial PSV @18:34:15.16 PS.seek 0ml @18:34:41.57 PSV.dial PBV @18:34:42.05 PS.seek 2.5ml @18:35:08.39 PSV.dial :waste1 @18:35:09.14 PS.seek 0ml @18:35:35.70 PSV.dial PSR @18:35:36.18 PSR.select :air @18:35:36.22 PS.seek 2.5ml @18:36:02.57 PSV.dial PTV @18:36:03.34 PBV.dial PSV @18:36:03.55 PS.seek 0ml @18:36:29.92 PSV.dial PBV @18:36:30.45 PS.seek 2.5ml @18:36:56.85 PSV.dial :waste1 @18:36:57.61 PS.seek 0ml @18:37:24.17 Garaging FlushPuck in Processing Clamp @18:37:24.26 Elbow.reconfigure :tight @18:37:24.33 Elbow.seek PC @18:37:24.47 Elbow.reconfigure :loose @18:37:24.60 Forearm.seek PC @18:37:25.56 Hand.close @18:37:26.18 PC.seek :open @18:37:30.91 Forearm.seek :retract @18:37:31.89 Elbow.reconfigure :tight @18:37:31.96 Elbow.seek :garage @18:37:33.34 Elbow.reconfigure :loose @18:37:33.46 Forearm.reconfigure :tight @18:37:33.53 Forearm.seek :garage @18:37:34.63 Hand.open @18:37:35.37 Forearm.reconfigure :loose @18:37:35.44 Forearm.seek :retract FlushPuck::Garage @18:37:43.96 -> exit @18:37:43.98 Session Ended @16:38:57.66UTC16-Oct-23 Session Begun @16:39:05.43 -> abort! 'mission complete' @16:39:05.45 CmdServer::Client::Error in dave -- Aborting Mission: mission complete @16:39:05.49 BadNews.email "mission complete",Subject: "DA_WCR_101223 ABORTED!" # @16:39:09.78 upsync @16:39:09.87 Session Ended + ESPwhich DA_WCR_101623.rb + fn=DA_WCR_101623.rb + IFS=: + set /home/esp/esp2/mission . + fullPath=/home/esp/esp2/mission/DA_WCR_101623.rb + [ -f /home/esp/esp2/mission/DA_WCR_101623.rb ] + realpath /home/esp/esp2/mission/DA_WCR_101623.rb + return 0 + scriptPath=/home/esp/esp2local/mission/DA_WCR_101623.rb + exec ruby /home/esp/esp2/lib/esp.rb /home/esp/esp2local/mission/DA_WCR_101623.rb @16:47:54.08UTC16-Oct-23
ESP2 v3.42 I2C Gateway v3.20 for core bus @16:47:56.89 Configuration for short puck jake 1/11/21 by brent@mbari.org /home/esp/esp2/type/shallow/jake/configure.rb @16:48:00.76 Polling Can every 10:00 @16:48:00.81 Cleanly exited at 16:44:19UTC16-Oct-23 @16:48:00.86 Loading /home/esp/esp2local/mission/DA_WCR_101623.rb mission @16:48:00.91 GoodNews.email "Starting DA_WCR_101623 w/pucks in tube 2\n{1 => 32,\n 2 => 1,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@16:48:00, 27% flow, 18.3C, 18% humidity, 14.6psia, 13.734V, 0.266A, 164.599Ah, 3.66W",Subject: "DA_WCR_101623 begun" @16:48:00.95
Preparing to sleep until + 00:00:05 @16:48:00.98 Asleep til + 00:00:05 @16:48:01.00 Continuing at 16:48 @16:48:01.28 Gate.power :core,:OFF @16:48:01.30 Gate.power :raw,:OFF @16:48:01.31 Shutdown @16:48:03.61 GoodNews.email "Then, in tube 2: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_101623.rb:14\n{1 => 32,\n 2 => 1,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@16:48:01, 27% flow, 18.3C, 18% humidity, 14.6psia, 13.734V, 0.272A, 164.599Ah, 3.74W",Subject: "Asleep until 16:48" @16:48:05.03 upsync @16:48:05.89
Powering core bus @16:48:07.82 upsync @16:48:11.60
Gate.power :raw,:ON @16:48:12.46 CS.configure :default @16:48:12.51 PS.configure :default @16:48:12.56 SS.configure :default @16:48:12.61 CC.configure :default @16:48:12.66 PC.configure :default @16:48:12.72 Forearm.configure :loose @16:48:12.77 Elbow.configure :loose @16:48:12.84 SC.configure :loose @16:48:12.89 SE.configure :default @16:48:12.93 CH.configure :default @16:48:12.97 PH.configure :default @16:48:13.01 All dwarf controllers running firmware version 3.85 @16:48:13.12 Valve.reset @16:48:13.43 Intake.select :close @16:48:13.45 PSV.dial :air @16:48:13.47 Exhaust.select :close @16:48:13.50 CTV.dial :air @16:48:13.65
Forearm.home.seek :retract @16:48:14.41 Hand.close @16:48:14.44
Elbow.reconfigure :loose @16:48:14.56 Elbow.jog 1500 @16:48:14.58 PS.home.seek 0ml @16:48:14.89 CSV.dial :waste1 @16:48:15.01
Elbow.reconfigure :loose @16:48:15.06 Elbow.home.seek :clear @16:48:15.61 SSV.dial :spit,avoiding: :exhaust @16:48:16.48 CS.reconfigure :fast @16:48:16.50 SS.home.seek 0ml @16:48:16.59 CS.home.seek 0ml @16:48:19.58
SE.home.seek :down @16:48:20.24 SC.reconfigure :tight @16:48:20.29 SC.home.seek :initial @16:48:22.93 PS.stop @16:48:48.62
SC.configure :limp @16:48:48.68 Forearm.seek :retract @16:48:48.74 Elbow.seek :hammer @16:48:49.79 Forearm.reconfigure :tight @16:48:49.84 Forearm.seek retracted @16:48:50.08 PC.home.seek :open @16:48:50.38
CC.home.seek :open @16:48:53.86 Phase [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] @16:48:53.88 Notice.email "At 16:48:53 began in tube 2: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_101623.rb:14\n{1 => 32,\n 2 => 1,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@16:48:53, 27% flow, 18.3C, 18% humidity, 14.6psia, 13.734V, 0.296A, 164.605Ah, 4.07W",Subject: "Started DA_WCR_101623 phase initialPurge; da [1000, 1000] #da and wsr are run simultanelously" @16:48:53.90
Starting initialPurge @16:48:53.93 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @16:48:53.99 Hand.open @16:48:54.72 Elbow.reconfigure :tight @16:48:54.77 Elbow.seek FlushPuck::Garage @16:48:56.63 Elbow.reconfigure :loose @16:48:56.87 Forearm.reconfigure :loose @16:48:56.97 Forearm.seek :garage @16:48:57.97 Hand.close @16:48:58.19 upsync @16:48:58.71
Forearm.seek :retract @16:48:59.73 Elbow.reconfigure :tight @16:48:59.78 Elbow.seek CC @16:49:02.74 Elbow.reconfigure :loose @16:49:02.85 Forearm.reconfigure :tight @16:49:02.90 Forearm.seek CC @16:49:04.15 CC.seek :closed @16:49:08.72 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.39 @16:49:08.79 Hand.open @16:49:09.53 Forearm.reconfigure :loose @16:49:09.58 Forearm.seek :retract @16:49:10.55 Flushing the Collection Valve::Manifold @16:49:10.68 CSV.dial CSR @16:49:11.96 Pulling 5ml of CSR.flush CSR.select :flush @16:49:12.08 CS.reconfigure :default @16:49:12.54 CS.seek 5ml @16:49:26.13 delay 5 seconds @16:49:31.15 CSR.select :air @16:49:31.31 CS.reconfigure :fast @16:49:31.36 CS.seek 7.499ml @16:49:38.19 CSV.dial :waste @16:49:39.47 CS.reconfigure :default @16:49:39.52 CS.seek 0ml @16:49:59.50 Purging external Intake @16:49:59.62 CSV.dial :flush @16:50:00.08 CS.reconfigure :fast @16:50:00.13 CS.seek 10ml @16:50:24.81 delay 5 seconds @16:50:29.84 CSV.dial CBV @16:50:29.88
CBV.dial CSV @16:50:30.66 CTV.dial :intake @16:50:31.45 Intake.select :open @16:50:31.57 CS.seek 0ml @16:50:55.28 Intake.select :close @16:50:55.38 CTV.dial :air @16:50:56.17 Flushing upper manifold @16:50:56.29 CSV.dial :flush @16:50:57.02 CS.seek 5ml @16:51:09.72 delay 5 seconds @16:51:14.74 CSV.dial :air @16:51:15.36 CS.seek 7ml @16:51:21.16 CSV.dial CBV @16:51:21.19
CBV.dial CSV @16:51:22.20 CTV.dial :air @16:51:22.29 CS.seek 0ml @16:51:38.97 delay 7 seconds @16:51:46.00 CS.seek 3ml @16:51:53.97 CSV.dial :waste @16:51:54.43 CS.seek 0ml @16:52:02.27 CSV.dial :flush @16:52:02.71 CS.seek 3ml @16:52:10.75 delay 5 seconds @16:52:15.77 CSV.dial :air @16:52:16.39 CS.seek 5ml @16:52:22.15 CSV.dial :air @16:52:22.24 CS.seek 0ml @16:52:34.37 delay 7 seconds @16:52:41.40 CS.seek 3ml @16:52:49.40 CSV.dial :waste @16:52:50.13 CS.seek 0ml @16:52:57.75 Garaging FlushPuck in Collection Clamp @16:52:57.82 Elbow.reconfigure :tight @16:52:57.87 Elbow.seek CC @16:52:58.20 Elbow.reconfigure :loose @16:52:58.30 Forearm.seek CC @16:52:59.33 Hand.close @16:52:59.96 CC.seek :open @16:53:05.02 Forearm.seek :retract @16:53:06.02 Elbow.reconfigure :tight @16:53:06.07 Elbow.seek :garage @16:53:08.96 Elbow.reconfigure :loose @16:53:09.06 Forearm.reconfigure :tight @16:53:09.11 Forearm.seek :garage @16:53:10.16 Hand.open @16:53:10.89 Forearm.reconfigure :loose @16:53:10.94 Forearm.seek :retract @16:53:11.93 da sampling at most 1000ml, wcr at most 1000ml @16:53:11.96 DAfans.email "Started da in tube 2 at 16:53:11UTC16-Oct-23\n{1 => 32,\n 2 => 1,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@16:48:53, 27% flow, 18.3C, 18% humidity, 14.6psia, 13.734V, 0.296A, 164.605Ah, 4.07W",Subject: "da sampling at most 1000ml, wcr at most 1000ml" @16:53:12.00
Loading sh1 puck from tube 2 into Collection Clamp @16:53:12.25 SC.reconfigure :tight @16:53:12.31 SC.seek 2 @16:53:15.90 upsync @16:53:19.08
SC.configure :limp @16:53:19.31 Elbow.reconfigure :tight @16:53:19.36 Elbow.seek 2 @16:53:21.32 Forearm.reconfigure :tight @16:53:21.37 Forearm.seek 2 + 450 counts @16:53:22.46 SE.reconfigure :pick @16:53:22.52 SE.seek :beamBreak @16:53:25.56 SE.configure :default @16:53:25.73 Elbow.seek 2 @16:53:25.93 Forearm.seek 2 + 300 counts @16:53:26.17 Forearm.reconfigure :loose @16:53:26.23 Hand.close @16:53:26.85 SE.seek :down @16:53:27.43
Forearm.seek :retract @16:53:28.44 Elbow.seek CC @16:53:29.73 SE.stop @16:53:30.09
Elbow.reconfigure :loose @16:53:30.19 Forearm.reconfigure :tight @16:53:30.24 Forearm.seek CC @16:53:31.48 CC.seek :closed @16:53:36.01 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.26 @16:53:36.02 Hand.open @16:53:36.65 Forearm.reconfigure :loose @16:53:36.69 Forearm.seek :retract @16:53:37.67 collectSample 1000ml w/bubble point of 24psi @16:53:37.69 Priming 7ml @16:53:37.70 Intake.select :close @16:53:37.71 Exhaust.select :close @16:53:37.72 SS.hold @16:53:37.93 Calibrating pressure and temperature sensors @16:53:37.96 CTV.dial :air @16:53:37.97 SSV.dial :spit,avoiding: :exhaust @16:53:38.05 SS.reconfigure :fast @16:53:38.09 SS.seek 5ml @16:53:41.14 SSV.dial CBV @16:53:41.16
CBV.dial SSV @16:53:42.50 delay 3.8 seconds @16:53:46.42 Sensor limits: Intake max 112.2psia; Outlet max 104.7psia @16:53:46.43 SSV.dial :spit @16:53:47.77 SS.seek 0ml @16:53:50.41 SSV.dial CBV @16:53:51.73 CTV.dial :intake @16:53:52.52 SSV.dial CBV @16:53:52.54
CBV.dial SSV @16:53:52.74 Allowing 40s for 7.0ml of remaining 7.0ml @16:53:52.76 Intake.select :open @16:53:54.92 Intake @14.5psia, vacuum from 9.0 to 12.0psi @16:53:54.94 SS.reconfigure :inhaling 2.20ml/s @16:53:54.99 SS.seek 7ml @16:54:02.35 Stroke completed -- waiting for pressure to equalize ... @16:54:02.36 SS.reconfigure :exhale @16:54:02.41 SS.hold @16:54:10.28 Intake.select :close @16:54:10.43 SSV.dial :exhaust @16:54:11.20 Exhaust.select :open @16:54:11.31 SS.seek 0ml @16:54:15.18 Exhaust.select :close @16:54:15.29 Sampling 1000.0ml @16:54:15.30 Intake.select :close @16:54:15.31 Exhaust.select :close @16:54:15.32 SS.reconfigure :default @16:54:15.36 SS.hold @16:54:15.56 CTV.dial :intake @16:54:15.64 SSV.dial CBV @16:54:15.69
CBV.dial SSV @16:54:16.43 Allowing 130s for 25.0ml of remaining 1000.0ml @16:54:16.44 Intake.select :open @16:54:18.60 Intake @15.3psia, vacuum from 9.8 to 12.8psi @16:54:18.62 SS.reconfigure :inhaling 2.20ml/s @16:54:18.67 SS.seek 25ml @16:54:26.94 Vacuum too high -- waiting up to 40 seconds @16:54:26.95 SS.reconfigure :exhale @16:54:27.00 SS.hold @16:54:31.50 Intake @15.6psia, vacuum from 10.0 to 13.0psi @16:54:31.51 SS.reconfigure :inhaling 1.76ml/s @16:54:31.56 SS.seek 25ml @16:54:38.72 Vacuum too high -- waiting up to 40 seconds @16:54:38.73 SS.reconfigure :exhale @16:54:39.21 SS.hold @16:54:43.30 Intake @15.9psia, vacuum from 10.0 to 13.0psi @16:54:43.31 SS.reconfigure :inhaling 1.41ml/s @16:54:43.37 SS.seek 25ml @16:54:45.77 Stroke completed -- waiting for pressure to equalize ... @16:54:45.78 SS.reconfigure :exhale @16:54:45.83 SS.hold @16:54:56.88 Intake.select :close @16:54:57.03 SSV.dial :exhaust @16:54:57.79 Exhaust.select :open @16:54:57.90 SS.seek 0ml @16:55:07.02 Exhaust.select :close @16:55:07.20 SSV.dial CBV @16:55:07.23
CBV.dial SSV @16:55:08.01 Allowing 130s for 25.0ml of remaining 975.0ml @16:55:08.02 Intake.select :open @16:55:10.19 Intake @15.7psia, vacuum from 10.0 to 13.0psi @16:55:10.20 SS.reconfigure :inhaling 1.41ml/s @16:55:10.25 SS.seek 25ml @16:55:23.59 Vacuum too high -- waiting up to 40 seconds @16:55:23.61 SS.reconfigure :exhale @16:55:23.66 SS.hold @16:55:27.95 Intake @15.9psia, vacuum from 10.0 to 13.0psi @16:55:27.97 SS.reconfigure :inhaling 1.13ml/s @16:55:28.01 SS.seek 25ml @16:55:37.24 Stroke completed -- waiting for pressure to equalize ... @16:55:37.25 SS.reconfigure :exhale @16:55:37.30 SS.hold @16:55:55.63 Intake.select :close @16:55:55.78 SSV.dial :exhaust @16:55:56.54 Exhaust.select :open @16:55:56.65 SS.seek 0ml @16:56:05.37 Exhaust.select :close @16:56:05.48 SSV.dial CBV @16:56:05.50
CBV.dial SSV @16:56:06.29 Allowing 130s for 25.0ml of remaining 950.0ml @16:56:06.30 Intake.select :open @16:56:08.46 Intake @15.7psia, vacuum from 10.0 to 13.0psi @16:56:08.48 SS.reconfigure :inhaling 1.13ml/s @16:56:08.53 SS.seek 25ml @16:56:33.38 Stroke completed -- waiting for pressure to equalize ... @16:56:33.39 SS.reconfigure :exhale @16:56:33.43 SS.hold @16:56:52.22 Intake.select :close @16:56:52.37 SSV.dial :exhaust @16:56:53.13 Exhaust.select :open @16:56:53.24 SS.seek 0ml @16:57:02.19 Exhaust.select :close @16:57:02.31 SSV.dial CBV @16:57:02.33
CBV.dial SSV @16:57:03.12 Allowing 130s for 25.0ml of remaining 925.0ml @16:57:03.13 Intake.select :open @16:57:05.29 Intake @15.8psia, vacuum from 10.0 to 13.0psi @16:57:05.31 SS.reconfigure :inhaling 1.13ml/s @16:57:05.35 SS.seek 25ml @16:57:29.69 Stroke completed -- waiting for pressure to equalize ... @16:57:29.70 SS.reconfigure :exhale @16:57:29.75 SS.hold @16:57:46.04 Intake.select :close @16:57:46.19 SSV.dial :exhaust @16:57:46.96 Exhaust.select :open @16:57:47.07 SS.seek 0ml @16:57:55.80 Exhaust.select :close @16:57:55.91 SSV.dial CBV @16:57:55.95
CBV.dial SSV @16:57:56.69 Allowing 130s for 25.0ml of remaining 900.0ml @16:57:56.70 Intake.select :open @16:57:59.29 Intake @15.7psia, vacuum from 10.0 to 13.0psi @16:57:59.31 SS.reconfigure :inhaling 1.13ml/s @16:57:59.35 SS.seek 25ml @16:58:23.77 Stroke completed -- waiting for pressure to equalize ... @16:58:23.78 SS.reconfigure :exhale @16:58:23.83 SS.hold @16:58:38.20 Intake.select :close @16:58:38.36 SSV.dial :exhaust @16:58:39.13 Exhaust.select :open @16:58:39.24 SS.seek 0ml @16:58:48.03 Exhaust.select :close @16:58:48.14 SSV.dial CBV @16:58:48.17
CBV.dial SSV @16:58:48.94 Allowing 130s for 25.0ml of remaining 875.0ml @16:58:48.95 Intake.select :open @16:58:51.12 Intake @15.7psia, vacuum from 10.0 to 13.0psi @16:58:51.13 SS.reconfigure :inhaling 1.13ml/s @16:58:51.18 SS.seek 25ml @16:59:15.89 Stroke completed -- waiting for pressure to equalize ... @16:59:15.90 SS.reconfigure :exhale @16:59:15.95 SS.hold @16:59:30.49 Intake.select :close @16:59:30.64 SSV.dial :exhaust @16:59:31.41 Exhaust.select :open @16:59:31.52 SS.seek 0ml @16:59:40.30 Exhaust.select :close @16:59:40.41 SSV.dial CBV @16:59:40.43
CBV.dial SSV @16:59:41.22 Allowing 130s for 25.0ml of remaining 850.0ml @16:59:41.23 Intake.select :open @16:59:43.39 Intake @15.7psia, vacuum from 10.0 to 13.0psi @16:59:43.41 SS.reconfigure :inhaling 1.13ml/s @16:59:43.45 SS.seek 25ml @17:00:08.36 Stroke completed -- waiting for pressure to equalize ... @17:00:08.37 SS.reconfigure :exhale @17:00:08.41 SS.hold @17:00:25.13 Intake.select :close @17:00:25.28 SSV.dial :exhaust @17:00:26.05 Exhaust.select :open @17:00:26.16 SS.seek 0ml @17:00:35.14 Exhaust.select :close @17:00:35.25 SSV.dial CBV @17:00:35.28
CBV.dial SSV @17:00:36.03 Allowing 130s for 25.0ml of remaining 825.0ml @17:00:36.04 Intake.select :open @17:00:38.21 Intake @15.6psia, vacuum from 10.0 to 13.0psi @17:00:38.23 SS.reconfigure :inhaling 1.13ml/s @17:00:38.28 SS.seek 25ml @17:01:02.75 Stroke completed -- waiting for pressure to equalize ... @17:01:02.76 SS.reconfigure :exhale @17:01:02.80 SS.hold @17:01:17.16 Intake.select :close @17:01:17.32 SSV.dial :exhaust @17:01:18.11 Exhaust.select :open @17:01:18.23 SS.seek 0ml @17:01:27.34 Exhaust.select :close @17:01:27.45 SSV.dial CBV @17:01:27.48
CBV.dial SSV @17:01:28.23 Allowing 130s for 25.0ml of remaining 800.0ml @17:01:28.25 Intake.select :open @17:01:30.41 Intake @15.6psia, vacuum from 10.0 to 13.0psi @17:01:30.43 SS.reconfigure :inhaling 1.13ml/s @17:01:30.48 SS.seek 25ml @17:01:54.70 Stroke completed -- waiting for pressure to equalize ... @17:01:54.71 SS.reconfigure :exhale @17:01:54.76 SS.hold @17:02:11.04 Intake.select :close @17:02:11.19 SSV.dial :exhaust @17:02:11.95 Exhaust.select :open @17:02:12.07 SS.seek 0ml @17:02:21.28 Exhaust.select :close @17:02:21.39 SSV.dial CBV @17:02:21.42
CBV.dial SSV @17:02:22.19 Allowing 130s for 25.0ml of remaining 775.0ml @17:02:22.20 Intake.select :open @17:02:24.36 Intake @15.5psia, vacuum from 10.0 to 13.0psi @17:02:24.38 SS.reconfigure :inhaling 1.13ml/s @17:02:24.43 SS.seek 25ml @17:02:48.36 Vacuum too high -- waiting up to 40 seconds @17:02:48.38 SS.reconfigure :exhale @17:02:48.43 SS.hold @17:02:58.31 Intake @15.3psia, vacuum from 9.8 to 12.8psi @17:02:58.33 SS.reconfigure :inhaling 0.91ml/s @17:02:58.37 SS.seek 25ml @17:02:59.35 Stroke completed -- waiting for pressure to equalize ... @17:02:59.37 SS.reconfigure :exhale @17:02:59.41 SS.hold @17:03:07.30 Intake.select :close @17:03:07.46 SSV.dial :exhaust @17:03:08.22 Exhaust.select :open @17:03:08.33 SS.seek 0ml @17:03:17.31 Exhaust.select :close @17:03:17.42 SSV.dial CBV @17:03:17.44
CBV.dial SSV @17:03:18.23 Allowing 130s for 25.0ml of remaining 750.0ml @17:03:18.24 Intake.select :open @17:03:20.41 Intake @15.4psia, vacuum from 9.9 to 12.9psi @17:03:20.43 SS.reconfigure :inhaling 0.91ml/s @17:03:20.48 SS.seek 25ml @17:03:49.78 Stroke completed -- waiting for pressure to equalize ... @17:03:49.79 SS.reconfigure :exhale @17:03:49.83 SS.hold @17:04:06.11 Intake.select :close @17:04:06.61 SSV.dial :exhaust @17:04:07.45 Exhaust.select :open @17:04:07.56 SS.seek 0ml @17:04:16.31 Exhaust.select :close @17:04:16.42 SSV.dial CBV @17:04:16.45
CBV.dial SSV @17:04:17.22 Allowing 130s for 25.0ml of remaining 725.0ml @17:04:17.23 Intake.select :open @17:04:19.39 Intake @15.4psia, vacuum from 9.9 to 12.9psi @17:04:19.41 SS.reconfigure :inhaling 0.91ml/s @17:04:19.46 SS.seek 25ml @17:04:49.67 Stroke completed -- waiting for pressure to equalize ... @17:04:49.68 SS.reconfigure :exhale @17:04:49.72 SS.hold @17:05:06.00 Intake.select :close @17:05:06.15 SSV.dial :exhaust @17:05:06.92 Exhaust.select :open @17:05:07.03 SS.seek 0ml @17:05:15.82 Exhaust.select :close @17:05:15.93 SSV.dial CBV @17:05:15.96
CBV.dial SSV @17:05:16.73 Allowing 130s for 25.0ml of remaining 700.0ml @17:05:16.74 Intake.select :open @17:05:18.90 Intake @15.3psia, vacuum from 9.8 to 12.8psi @17:05:18.92 SS.reconfigure :inhaling 0.91ml/s @17:05:18.97 SS.seek 25ml @17:05:49.33 Stroke completed -- waiting for pressure to equalize ... @17:05:49.34 SS.reconfigure :exhale @17:05:49.39 SS.hold @17:06:07.75 Intake.select :close @17:06:07.91 SSV.dial :exhaust @17:06:08.67 Exhaust.select :open @17:06:08.78 SS.seek 0ml @17:06:17.62 Exhaust.select :close @17:06:17.73 SSV.dial CBV @17:06:17.75
CBV.dial SSV @17:06:18.51 Allowing 130s for 25.0ml of remaining 675.0ml @17:06:18.52 Intake.select :open @17:06:20.68 Intake @15.1psia, vacuum from 9.6 to 12.6psi @17:06:20.70 SS.reconfigure :inhaling 0.91ml/s @17:06:20.75 SS.seek 25ml @17:06:45.89 Vacuum too high -- waiting up to 40 seconds @17:06:45.90 SS.reconfigure :exhale @17:06:45.95 SS.hold @17:06:57.42 Intake @15.6psia, vacuum from 10.0 to 13.0psi @17:06:57.44 SS.reconfigure :inhaling 0.73ml/s @17:06:57.48 SS.seek 25ml @17:07:03.52 Stroke completed -- waiting for pressure to equalize ... @17:07:03.53 SS.reconfigure :exhale @17:07:03.58 SS.hold @17:07:13.25 Intake.select :close @17:07:13.76 SSV.dial :exhaust @17:07:14.57 Exhaust.select :open @17:07:14.68 SS.seek 0ml @17:07:23.66 Exhaust.select :close @17:07:23.78 SSV.dial CBV @17:07:23.80
CBV.dial SSV @17:07:24.57 Allowing 130s for 25.0ml of remaining 650.0ml @17:07:24.58 Intake.select :open @17:07:26.75 Intake @15.2psia, vacuum from 9.7 to 12.7psi @17:07:26.77 SS.reconfigure :inhaling 0.73ml/s @17:07:26.82 SS.seek 25ml @17:08:02.60 Stroke completed -- waiting for pressure to equalize ... @17:08:02.61 SS.reconfigure :exhale @17:08:02.65 SS.hold @17:08:20.99 Intake.select :close @17:08:21.14 SSV.dial :exhaust @17:08:21.90 Exhaust.select :open @17:08:22.01 SS.seek 0ml @17:08:31.04 Exhaust.select :close @17:08:31.15 SSV.dial CBV @17:08:31.19
CBV.dial SSV @17:08:31.93 Allowing 130s for 25.0ml of remaining 625.0ml @17:08:31.94 Intake.select :open @17:08:34.53 Intake @15.2psia, vacuum from 9.7 to 12.7psi @17:08:34.54 SS.reconfigure :inhaling 0.73ml/s @17:08:34.59 SS.seek 25ml @17:09:10.66 Stroke completed -- waiting for pressure to equalize ... @17:09:10.67 SS.reconfigure :exhale @17:09:10.72 SS.hold @17:09:31.02 Intake.select :close @17:09:31.17 SSV.dial :exhaust @17:09:31.93 Exhaust.select :open @17:09:32.05 SS.seek 0ml @17:09:40.88 Exhaust.select :close @17:09:40.99 SSV.dial CBV @17:09:41.01
CBV.dial SSV @17:09:41.80 Allowing 130s for 25.0ml of remaining 600.0ml @17:09:41.81 Intake.select :open @17:09:43.98 Intake @15.3psia, vacuum from 9.8 to 12.8psi @17:09:44.00 SS.reconfigure :inhaling 0.73ml/s @17:09:44.04 SS.seek 25ml @17:10:20.74 Stroke completed -- waiting for pressure to equalize ... @17:10:20.75 SS.reconfigure :exhale @17:10:20.79 SS.hold @17:10:45.73 Intake.select :close @17:10:45.89 SSV.dial :exhaust @17:10:46.64 Exhaust.select :open @17:10:46.76 SS.seek 0ml @17:10:55.80 Exhaust.select :close @17:10:55.91 SSV.dial CBV @17:10:55.95
CBV.dial SSV @17:10:56.69 Allowing 130s for 25.0ml of remaining 575.0ml @17:10:56.70 Intake.select :open @17:10:58.86 Intake @15.2psia, vacuum from 9.7 to 12.7psi @17:10:58.88 SS.reconfigure :inhaling 0.73ml/s @17:10:58.92 SS.seek 25ml @17:11:28.07 Vacuum too high -- waiting up to 40 seconds @17:11:28.09 SS.reconfigure :exhale @17:11:28.14 SS.hold @17:11:42.94 Intake @15.9psia, vacuum from 10.0 to 13.0psi @17:11:42.96 SS.reconfigure :inhaling 0.59ml/s @17:11:43.00 SS.seek 25ml @17:11:52.17 Stroke completed -- waiting for pressure to equalize ... @17:11:52.18 SS.reconfigure :exhale @17:11:52.23 SS.hold @17:12:06.57 Intake.select :close @17:12:06.72 SSV.dial :exhaust @17:12:07.49 Exhaust.select :open @17:12:07.60 SS.seek 0ml @17:12:16.41 Exhaust.select :close @17:12:16.52 SSV.dial CBV @17:12:16.54
CBV.dial SSV @17:12:17.30 Allowing 130s for 25.0ml of remaining 550.0ml @17:12:17.32 Intake.select :open @17:12:19.48 Intake @15.1psia, vacuum from 9.6 to 12.6psi @17:12:19.50 SS.reconfigure :inhaling 0.59ml/s @17:12:19.55 SS.seek 25ml @17:13:04.48 Stroke completed -- waiting for pressure to equalize ... @17:13:04.49 SS.reconfigure :exhale @17:13:04.54 SS.hold @17:13:27.38 Intake.select :close @17:13:27.53 SSV.dial :exhaust @17:13:28.28 Exhaust.select :open @17:13:28.39 SS.seek 0ml @17:13:37.19 Exhaust.select :close @17:13:37.30 SSV.dial CBV @17:13:37.33
CBV.dial SSV @17:13:38.09 Allowing 130s for 25.0ml of remaining 525.0ml @17:13:38.11 Intake.select :open @17:13:40.27 Intake @15.1psia, vacuum from 9.6 to 12.6psi @17:13:40.29 SS.reconfigure :inhaling 0.59ml/s @17:13:40.34 SS.seek 25ml @17:14:25.17 Stroke completed -- waiting for pressure to equalize ... @17:14:25.18 SS.reconfigure :exhale @17:14:25.23 SS.hold @17:14:51.75 Intake.select :close @17:14:51.90 SSV.dial :exhaust @17:14:52.68 Exhaust.select :open @17:14:52.79 SS.seek 0ml @17:15:01.78 Exhaust.select :close @17:15:01.89 SSV.dial CBV @17:15:01.92
CBV.dial SSV @17:15:02.70 Allowing 130s for 25.0ml of remaining 500.0ml @17:15:02.71 Intake.select :open @17:15:04.89 Intake @15.2psia, vacuum from 9.7 to 12.7psi @17:15:04.90 SS.reconfigure :inhaling 0.59ml/s @17:15:04.95 SS.seek 25ml @17:15:47.19 Vacuum too high -- waiting up to 40 seconds @17:15:47.20 SS.reconfigure :exhale @17:15:47.26 SS.hold @17:16:03.77 Intake @15.9psia, vacuum from 10.0 to 13.0psi @17:16:03.79 SS.reconfigure :inhaling 0.47ml/s @17:16:03.84 SS.seek 25ml @17:16:06.65 Stroke completed -- waiting for pressure to equalize ... @17:16:06.66 SS.reconfigure :exhale @17:16:06.71 SS.hold @17:16:21.07 Intake.select :close @17:16:21.22 SSV.dial :exhaust @17:16:21.98 Exhaust.select :open @17:16:22.09 SS.seek 0ml @17:16:30.90 Exhaust.select :close @17:16:31.01 SSV.dial CBV @17:16:31.03
CBV.dial SSV @17:16:31.81 Allowing 130s for 25.0ml of remaining 475.0ml @17:16:31.82 Intake.select :open @17:16:33.98 Intake @15.1psia, vacuum from 9.6 to 12.6psi @17:16:34.00 SS.reconfigure :inhaling 0.47ml/s @17:16:34.05 SS.seek 25ml @17:17:30.12 Stroke completed -- waiting for pressure to equalize ... @17:17:30.13 SS.reconfigure :exhale @17:17:30.18 SS.hold @17:17:56.99 Intake.select :close @17:17:57.15 SSV.dial :exhaust @17:17:57.93 Exhaust.select :open @17:17:58.04 SS.seek 0ml @17:18:06.99 Exhaust.select :close @17:18:07.10 SSV.dial CBV @17:18:07.13
CBV.dial SSV @17:18:07.90 Allowing 130s for 25.0ml of remaining 450.0ml @17:18:07.91 Intake.select :open @17:18:10.07 Intake @15.1psia, vacuum from 9.6 to 12.6psi @17:18:10.09 SS.reconfigure :inhaling 0.47ml/s @17:18:10.14 SS.seek 25ml @17:19:05.85 Stroke completed -- waiting for pressure to equalize ... @17:19:05.86 SS.reconfigure :exhale @17:19:05.91 SS.hold @17:19:34.88 Intake.select :close @17:19:35.03 SSV.dial :exhaust @17:19:35.79 Exhaust.select :open @17:19:35.90 SS.seek 0ml @17:19:44.65 Exhaust.select :close @17:19:44.76 SSV.dial CBV @17:19:44.79
CBV.dial SSV @17:19:45.54 Allowing 130s for 25.0ml of remaining 425.0ml @17:19:45.55 Intake.select :open @17:19:47.72 Intake @15.1psia, vacuum from 9.6 to 12.6psi @17:19:47.74 SS.reconfigure :inhaling 0.47ml/s @17:19:47.78 SS.seek 25ml @17:20:43.94 Stroke completed -- waiting for pressure to equalize ... @17:20:44.30 SS.reconfigure :exhale @17:20:44.42 SS.hold @17:21:17.08 Intake.select :close @17:21:17.23 SSV.dial :exhaust @17:21:17.98 Exhaust.select :open @17:21:18.10 SS.seek 0ml @17:21:27.19 Exhaust.select :close @17:21:27.30 SSV.dial CBV @17:21:27.33
CBV.dial SSV @17:21:28.52 Allowing 130s for 25.0ml of remaining 400.0ml @17:21:28.54 Intake.select :open @17:21:30.70 Intake @15.1psia, vacuum from 9.6 to 12.6psi @17:21:30.72 SS.reconfigure :inhaling 0.47ml/s @17:21:30.77 SS.seek 25ml @17:22:25.83 Stroke completed -- waiting for pressure to equalize ... @17:22:25.84 SS.reconfigure :exhale @17:22:25.89 SS.hold @17:23:02.56 Intake.select :close @17:23:02.72 SSV.dial :exhaust @17:23:03.47 Exhaust.select :open @17:23:03.59 SS.seek 0ml @17:23:12.38 Exhaust.select :close @17:23:12.49 SSV.dial CBV @17:23:12.52
CBV.dial SSV @17:23:13.29 Allowing 130s for 25.0ml of remaining 375.0ml @17:23:13.30 Intake.select :open @17:23:15.46 Intake @15.1psia, vacuum from 9.6 to 12.6psi @17:23:15.48 SS.reconfigure :inhaling 0.47ml/s @17:23:15.53 SS.seek 25ml @17:24:00.31 Vacuum too high -- waiting up to 40 seconds @17:24:00.32 SS.reconfigure :exhale @17:24:00.37 SS.hold @17:24:18.86 Intake @15.6psia, vacuum from 10.0 to 13.0psi @17:24:18.87 SS.reconfigure :inhaling 0.38ml/s @17:24:18.92 SS.seek 25ml @17:24:33.99 Stroke completed -- waiting for pressure to equalize ... @17:24:34.00 SS.reconfigure :exhale @17:24:34.05 SS.hold @17:24:56.50 Intake.select :close @17:24:56.66 SSV.dial :exhaust @17:24:57.42 Exhaust.select :open @17:24:57.54 SS.seek 0ml @17:25:06.35 Exhaust.select :close @17:25:06.46 SSV.dial CBV @17:25:06.48
CBV.dial SSV @17:25:07.27 Allowing 130s for 25.0ml of remaining 350.0ml @17:25:07.28 Intake.select :open @17:25:09.44 Intake @15.1psia, vacuum from 9.6 to 12.6psi @17:25:09.46 SS.reconfigure :inhaling 0.38ml/s @17:25:09.51 SS.seek 25ml @17:26:19.51 Stroke completed -- waiting for pressure to equalize ... @17:26:19.52 SS.reconfigure :exhale @17:26:19.57 SS.hold @17:26:54.65 Intake.select :close @17:26:54.81 SSV.dial :exhaust @17:26:55.56 Exhaust.select :open @17:26:55.68 SS.seek 0ml @17:27:04.50 Exhaust.select :close @17:27:04.61 SSV.dial CBV @17:27:04.64
CBV.dial SSV @17:27:05.84 Allowing 130s for 25.0ml of remaining 325.0ml @17:27:05.86 Intake.select :open @17:27:08.02 Intake @15.1psia, vacuum from 9.6 to 12.6psi @17:27:08.04 SS.reconfigure :inhaling 0.38ml/s @17:27:08.09 SS.seek 25ml @17:28:18.23 Stroke completed -- waiting for pressure to equalize ... @17:28:18.24 SS.reconfigure :exhale @17:28:18.29 SS.hold @17:28:58.46 Intake.select :close @17:28:58.61 SSV.dial :exhaust @17:28:59.37 Exhaust.select :open @17:28:59.49 SS.seek 0ml @17:29:08.56 Exhaust.select :close @17:29:08.67 SSV.dial CBV @17:29:08.69
CBV.dial SSV @17:29:09.48 Allowing 130s for 25.0ml of remaining 300.0ml @17:29:09.49 Intake.select :open @17:29:11.65 Intake @15.1psia, vacuum from 9.6 to 12.6psi @17:29:11.67 SS.reconfigure :inhaling 0.38ml/s @17:29:11.72 SS.seek 25ml @17:30:20.58 Vacuum too high -- waiting up to 40 seconds @17:30:20.59 SS.reconfigure :exhale @17:30:20.65 SS.hold @17:30:45.26 Intake @15.5psia, vacuum from 10.0 to 13.0psi @17:30:45.28 SS.reconfigure :inhaling 0.31ml/s @17:30:45.32 SS.seek 25ml @17:30:46.37 Stroke completed -- waiting for pressure to equalize ... @17:30:46.38 SS.reconfigure :exhale @17:30:46.42 SS.hold @17:31:06.82 Intake.select :close @17:31:06.98 SSV.dial :exhaust @17:31:07.73 Exhaust.select :open @17:31:07.84 SS.seek 0ml @17:31:16.82 Exhaust.select :close @17:31:16.93 SSV.dial CBV @17:31:16.96
CBV.dial SSV @17:31:17.73 Allowing 130s for 25.0ml of remaining 275.0ml @17:31:18.13 Intake.select :open @17:31:20.33 Intake @15.1psia, vacuum from 9.6 to 12.6psi @17:31:20.35 SS.reconfigure :inhaling 0.31ml/s @17:31:20.40 SS.seek 25ml @17:32:50.68 Stroke completed -- waiting for pressure to equalize ... @17:32:50.69 SS.reconfigure :exhale @17:32:50.74 SS.hold @17:33:28.14 SS.reconfigure :default @17:33:28.19 SS.hold @17:33:28.25 Intake.select :close @17:33:28.41 ShallowSampler::Clogged in MAIN -- Filtering too Slowly after 750.0ml @17:33:28.42 SSV.dial :exhaust @17:33:29.18 Exhaust.select :open @17:33:29.29 SS.reconfigure :exhale @17:33:29.34 SS.seek 0ml @17:33:38.08 Exhaust.select :close @17:33:38.19 Sampled 750.0ml @17:33:38.21 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @17:33:38.22 Intake.select :close @17:33:38.29 CSV.dial :waste @17:33:38.44 SSV.dial CBV,avoiding: :exhaust @17:33:38.47 CS.seek 0ml @17:33:38.51
CBV.dial SSV @17:33:38.64 CSV.dial :air @17:33:39.38 CS.seek 10ml @17:33:40.42
CTV.dial :air @17:33:41.21 delay 15 seconds @17:33:56.23 SSV.dial :spit,avoiding: :exhaust @17:33:57.54 CTV.dial CSV,avoiding: :intake @17:33:57.58
SS.seek 0ml @17:33:57.71 SSV.dial CBV,avoiding: :exhaust @17:34:04.09 SSV.dial between CBV and plug @17:34:04.56 CBV.dial between SSV and waste2 @17:34:04.94 CTV.dial CSV,avoiding: :air @17:34:04.97
CSV.dial CTV @17:34:05.46 CS.reconfigure :pressurize @17:34:05.51 CS.seek 0ml @17:34:24.15 BubblePoint: Intake @24.2psia, Outlet @0.5psia @17:34:24.16 CBV.dial SSV @17:34:24.49 SS.reconfigure :default @17:34:24.54 SS.seek 10ml @17:34:30.18 delay 15 seconds @17:34:45.31 Evacuated: Intake @23.9psia, Outlet @23.9psia @17:34:45.32 SSV.dial :spit,avoiding: :exhaust @17:34:46.36 SS.reconfigure :exhale @17:34:46.40 SS.seek 0ml @17:34:51.31 SS.reconfigure :fast @17:34:51.35 SS.seek 5ml @17:34:54.15 SSV.dial CBV,avoiding: :exhaust @17:34:54.18
CBV.dial SSV @17:34:55.51 CTV.dial :air,avoiding: :intake @17:34:56.85 CSV.dial CTV @17:34:56.90
SS.seek 10ml @17:34:58.42 CSV.dial :air @17:34:58.85 CS.reconfigure :fast @17:34:58.90 CS.seek 0ml @17:34:59.82
SSV.dial :spit,avoiding: :exhaust @17:35:01.14 SS.reconfigure :exhale @17:35:01.19 SS.seek 0ml @17:35:06.17 SS.reconfigure :fast @17:35:06.23 SS.seek 5ml @17:35:06.49 CS.stop @17:35:09.17 SSV.dial CBV,avoiding: :exhaust @17:35:09.19
CBV.dial SSV @17:35:11.53 SSV.dial :spit,avoiding: :exhaust @17:35:12.87 SS.reconfigure :default @17:35:12.92 SS.seek 0ml @17:35:15.93 SS.stop @17:35:15.97 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @17:35:15.98 Intake.select :close @17:35:16.05 CSV.dial :waste @17:35:16.18 SSV.dial CBV,avoiding: :exhaust @17:35:16.22
CBV.dial SSV @17:35:16.83 CS.seek 0ml @17:35:16.89 CSV.dial :air @17:35:17.52
CTV.dial :air @17:35:17.60 delay 15 seconds @17:35:17.64 CS.seek 10ml @17:35:32.62
SSV.dial :spit,avoiding: :exhaust @17:35:33.93 CTV.dial CSV,avoiding: :intake @17:35:33.97
SS.reconfigure :exhale @17:35:34.07 SS.seek 0ml @17:35:34.45 SSV.dial CBV,avoiding: :exhaust @17:35:42.08 SSV.dial between CBV and plug @17:35:42.57 CBV.dial between SSV and waste2 @17:35:42.95 CSV.dial between air and CTV @17:35:43.26 CS.seek 7.573ml @17:35:49.68 CTV.dial CSV,avoiding: :air @17:35:49.71
CSV.dial CTV @17:35:50.05 CS.reconfigure :pressurize @17:35:50.10 CS.seek 0ml @17:36:06.03 BubblePoint: Intake @24.3psia, Outlet @3.4psia CBV.dial SSV @17:36:06.37 SS.reconfigure :default @17:36:06.42 SS.seek 10ml @17:36:11.96 delay 15 seconds @17:36:27.09 Evacuated: Intake @24.0psia, Outlet @24.1psia @17:36:27.10 SSV.dial :spit,avoiding: :exhaust @17:36:28.13 SS.reconfigure :exhale @17:36:28.61 SS.seek 0ml @17:36:33.63 SS.reconfigure :fast @17:36:33.67 SS.seek 5ml @17:36:36.49 SSV.dial CBV,avoiding: :exhaust @17:36:36.52
CBV.dial SSV @17:36:37.87 CTV.dial :air,avoiding: :intake @17:36:39.21 CSV.dial CTV @17:36:39.25
SS.seek 10ml @17:36:40.44 CSV.dial :air @17:36:40.88 CS.reconfigure :fast @17:36:40.93 CS.seek 0ml @17:36:41.72
SSV.dial :spit,avoiding: :exhaust @17:36:43.06 SS.reconfigure :exhale @17:36:43.10 SS.seek 0ml @17:36:45.30 CS.stop @17:36:48.11
SS.reconfigure :fast @17:36:48.16 SS.seek 5ml @17:36:50.91 SSV.dial CBV,avoiding: :exhaust @17:36:50.93
CBV.dial SSV @17:36:53.26 SSV.dial :spit,avoiding: :exhaust @17:36:54.59 SS.reconfigure :default @17:36:54.63 SS.seek 0ml @17:36:57.72 SS.stop @17:36:57.76 Lysing: Adding 2.0ml AqMeOH @17:36:57.77 CBV.dial between CSV and waste1,avoiding: :DBV @17:36:59.27 CTV.dial CSV @17:37:00.60 CSV.dial CSR @17:37:01.38 Pulling 1ml of CSR.AqMeOH @17:37:01.39 CSR.select :AqMeOH @17:37:01.51 CS.reconfigure :default @17:37:01.55 CS.seek 1ml @17:37:04.92 delay 5 seconds @17:37:09.94 CSR.select :air @17:37:10.11 CS.seek 6ml @17:37:23.77 delay 2 seconds @17:37:25.78 CSV.dial :waste2 @17:37:26.22 CS.reconfigure :fast @17:37:26.28 CS.seek 0ml @17:37:40.64 CSV.dial CSR @17:37:41.09 Pulling 2ml of CSR.AqMeOH @17:37:41.10 CSR.select :AqMeOH @17:37:41.22 CS.reconfigure :default @17:37:41.27 CS.seek 2ml @17:37:47.23 delay 5 seconds @17:37:52.25 CSR.select :air @17:37:52.42 CS.seek 5ml @17:38:00.95 delay 2 seconds @17:38:02.97 CSV.dial CTV @17:38:03.41 CS.seek 1ml @17:38:14.96 Before lysing Collection Puck Heater at 16.9C/29.3psia @17:38:14.98 Heating Puck.from 2, :sh1 in Collection Clamp to 70C for 10:00 @17:38:15.00 CH.seek 70C,8:00 @17:40:24.82 CH.hold 10:00,70C @17:40:25.01 Begun lysing Collection Puck Heater at 68C/35.5psia @17:50:25.02 Ended lysing Collection Puck Heater at 70C/34.8psia @17:50:25.03 CS.seek 0ml @17:50:28.41 CTV.dial between spare and air,avoiding: :intake @17:50:29.44 CSV.dial :air @17:50:29.89 CS.seek 5ml @17:50:43.50 CSV.dial CTV @17:50:43.96 delay 2 seconds @17:50:45.97 CSV.dial :air @17:50:46.43 delay 2 seconds @17:50:48.44 CSV.dial CTV @17:50:48.90 delay 2 seconds @17:50:50.91 CSV.dial :air @17:50:51.36 delay 2 seconds @17:50:53.39 CS.seek 0ml @17:51:07.05 Puck @77.8psia after final lysis pressurization @17:51:07.07 Recovering Lysate into Collection syringe @17:51:07.08 CSV.dial CBV @17:51:08.08 CBV.dial CSV @17:51:08.46 CS.seek 5ml @17:51:22.25 delay 5 seconds @17:51:27.27 CTV.dial :air @17:51:27.80 CS.seek 7ml @17:51:33.78 delay 5 seconds @17:51:38.80 CSV.dial :air2 @17:51:39.24 CS.seek 10ml @17:51:47.91 CSV.dial between air and CTV,avoiding: CBV @17:51:49.07 Lysing Complete @17:51:49.13 Storing sh1 puck in Collection Clamp to tube 1 @17:51:49.31 SC.reconfigure :tight @17:51:49.35 SC.seek 1 @17:51:52.90 SC.configure :limp @17:51:53.12 Elbow.reconfigure :tight @17:51:53.17 Elbow.seek 1 @17:51:55.36 Forearm.reconfigure :tight @17:51:55.42 Forearm.seek 1 + 450 counts @17:51:56.48 SE.reconfigure :pick @17:51:56.54 SE.seek :beamBreak @17:51:57.65 SE.configure :default @17:51:57.82 Elbow.reconfigure :limp @17:51:57.87 Forearm.reconfigure :loose @17:51:57.93 Forearm.seek 1 @17:51:58.15 Elbow.hold @17:51:58.22 Elbow.reconfigure :tight @17:51:58.32 SE.seek 31.02 Pucks below Top Plate @17:51:58.86 Forearm.force -20 @17:51:58.90 Forearm.seek 1 + 305 counts @17:51:59.21 Forearm.reconfigure :tight @17:51:59.26 Forearm.seek 1 + 450 counts @17:51:59.46 SE.seek 32.02 Pucks below Top Plate @17:52:00.01 Forearm.reconfigure :loose @17:52:00.05 Forearm.seek :retract @17:52:01.02 Elbow.seek CC @17:52:02.72 Elbow.reconfigure :loose @17:52:02.83 Forearm.seek CC @17:52:03.81 Hand.close @17:52:04.41 CC.seek :open @17:52:09.15 Forearm.seek :retract @17:52:10.17 Elbow.reconfigure :tight @17:52:10.22 Elbow.seek 1 @17:52:11.99 Elbow.reconfigure :limp @17:52:12.04 Forearm.seek 1 @17:52:13.09 Elbow.hold @17:52:13.16 Elbow.reconfigure :tight @17:52:13.20 Hand.open @17:52:14.34 Forearm.force -20 @17:52:14.38 Forearm.seek 1 + 317 counts @17:52:14.67 Forearm.reconfigure :tight @17:52:14.72 Forearm.seek 1 + 450 counts @17:52:14.98 SE.seek :down @17:52:15.51 SE.stop @17:52:16.12
Forearm.reconfigure :loose @17:52:16.17 Forearm.seek :retract @17:52:17.17 Tranferring DA @17:52:17.18 Beginning samplePassC @17:52:17.19 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @17:52:17.38 Elbow.seek FlushPuck::Garage @17:52:19.18 Elbow.reconfigure :loose @17:52:19.30 Forearm.seek :garage @17:52:20.27 Hand.close @17:52:21.03 Forearm.seek :retract @17:52:21.99 Elbow.reconfigure :tight @17:52:22.03 Elbow.seek CC @17:52:25.00 Elbow.reconfigure :loose @17:52:25.10 Forearm.reconfigure :tight @17:52:25.15 Forearm.seek CC @17:52:26.41 CC.seek :closed @17:52:31.50 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.35 @17:52:31.57 Hand.open @17:52:32.22 Forearm.reconfigure :loose @17:52:32.26 Forearm.seek :retract @17:52:33.26 PSV.dial PSR @17:52:34.64 Pulling 1.5ml of PSR.flush @17:52:34.65 PSR.select :flush @17:52:34.78 PS.seek 1.5ml @17:52:50.98 delay 5 seconds @17:52:55.99 PSV.dial :waste1 @17:52:56.47 PS.seek 0ml @17:53:12.63 PSV.dial PSR @17:53:13.10 PSR.select :air @17:53:13.22 PS.seek 2.5ml @17:53:39.60 PRV.dial CBV,avoiding: :ab2 @17:53:39.63
CBV.dial PRV @17:53:41.36 PSV.dial :mixloop @17:53:42.40 CTV.dial :air,avoiding: :intake @17:53:42.47 PTV.dial :PRVmixing @17:53:43.79 PS.seek 0ml @17:54:10.35 PSV.dial :air @17:54:10.83 PS.seek 2.5ml @17:54:37.19 PSV.dial :mixloop @17:54:37.67 PS.seek 0ml @17:55:03.97 PSV.dial :air @17:55:04.45 PS.seek 2.5ml @17:55:30.84 PSV.dial :mixloop @17:55:31.32 PS.seek 0ml @17:55:57.63 Garaging FlushPuck in Collection Clamp @17:55:57.70 Elbow.reconfigure :tight @17:55:57.74 Elbow.seek CC @17:55:58.08 Elbow.reconfigure :loose @17:55:58.19 Forearm.seek CC @17:55:59.24 Hand.close @17:55:59.90 CC.seek :open @17:56:04.52 Forearm.seek :retract @17:56:05.97 Elbow.reconfigure :tight @17:56:06.02 Elbow.seek :garage @17:56:08.91 Elbow.reconfigure :loose @17:56:09.01 Forearm.reconfigure :tight @17:56:09.06 Forearm.seek :garage @17:56:10.12 Hand.open @17:56:10.84 Forearm.reconfigure :loose @17:56:10.89 Forearm.seek :retract @17:56:11.87 PSV.dial :mixloop @17:56:11.96 PTV.dial :PRVmixing @17:56:11.97 PRV.dial CBV @17:56:12.00
CBV.dial PRV @17:56:12.20 CTV.dial :air,avoiding: :intake @17:56:12.28 Loading lyfil puck from tube 3 into Collection Clamp @17:56:12.52 SC.reconfigure :tight @17:56:12.57 SC.seek 3 @17:56:19.33 SC.configure :limp @17:56:19.55 Elbow.reconfigure :tight @17:56:19.60 Elbow.seek 3 @17:56:21.52 Forearm.reconfigure :tight @17:56:21.57 Forearm.seek 3 + 450 counts @17:56:22.58 SE.reconfigure :pick @17:56:22.64 SE.seek :beamBreak @17:56:23.65 SE.configure :default @17:56:23.81 Elbow.seek 3 @17:56:24.00 Forearm.seek 3 + 300 counts @17:56:24.24 Forearm.reconfigure :loose @17:56:24.29 Hand.close @17:56:24.93 SE.seek :down @17:56:25.59 SE.stop @17:56:25.61
Forearm.seek :retract @17:56:26.56 Elbow.seek CC @17:56:28.14 Elbow.reconfigure :loose @17:56:28.25 Forearm.reconfigure :tight @17:56:28.29 Forearm.seek CC @17:56:29.55 CC.seek :closed @17:56:34.06 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.13 @17:56:34.07 Hand.open @17:56:34.71 Forearm.reconfigure :loose @17:56:34.76 Forearm.seek :retract @17:56:35.73 CTV.dial CSV,avoiding: :intake @17:56:37.09 CSV.dial CTV @17:56:37.42 PS.seek 2.5ml @17:57:03.84 CS.seek 4.5ml @17:57:18.68 delay 8 seconds @17:57:26.71 PSV.dial :air @17:57:27.17 delay 2 seconds @17:57:29.19 PSV.dial :waste2 @17:57:29.93 PS.seek 0ml @17:57:56.31 CBV.dial between PRV and waste1 @17:57:56.64 CSV.dial :air @17:57:57.10 CS.seek 10ml @17:58:12.02 CSV.dial CTV @17:58:12.48 CS.seek 0ml @17:58:38.87 CBV.dial :waste1 @17:58:39.18 delay 5 seconds @17:58:44.20 CTV.dial :air,avoiding: :intake @17:58:45.60 Storing lyfil puck in Collection Clamp to tube 2 @17:58:46.23 SC.reconfigure :tight @17:58:46.28 SC.seek 2 @17:58:49.82 SC.configure :limp @17:58:49.98 SE.reconfigure :place @17:58:50.06
Elbow.reconfigure :tight @17:58:50.10 SE.seek :beamBreak @17:58:50.13
Elbow.seek CC @17:58:50.45 Elbow.reconfigure :loose @17:58:50.56 Forearm.seek CC @17:58:51.59 Hand.close @17:58:52.15 CC.seek :open @17:58:52.97 SE.configure :default @17:58:53.03 SE.seek :place @17:58:56.88
Forearm.seek :retract @17:58:57.85 Elbow.reconfigure :tight @17:58:57.90 Elbow.seek 2 @17:58:59.54 Elbow.reconfigure :limp @17:58:59.59 Forearm.seek 2 @17:59:00.57 Elbow.hold @17:59:00.64 Elbow.reconfigure :tight @17:59:00.68 Hand.open @17:59:01.39 Forearm.force -20 @17:59:01.43 Forearm.seek 2 + 308 counts @17:59:01.75 Forearm.reconfigure :tight @17:59:01.79 Forearm.seek 2 + 450 counts @17:59:02.06 SE.seek :down @17:59:03.63
Forearm.reconfigure :loose @17:59:03.68 Forearm.seek :retract @17:59:04.77 SE.stop @17:59:04.81
PSV.dial :air @17:59:05.57 PS.seek 2.5ml @17:59:31.87 delay 1 second @17:59:32.89 PSV.dial :mixloop @17:59:33.34 delay 1 second @17:59:34.36 PSV.dial :air @17:59:34.83 delay 1 second @17:59:35.84 PSV.dial :mixloop @17:59:36.31 delay 1 second @17:59:37.33 PSV.dial :air @17:59:37.80 delay 1 second @17:59:38.82 PS.seek 0ml @18:00:05.09 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @18:00:05.28 Elbow.seek FlushPuck::Garage @18:00:07.12 Elbow.reconfigure :loose @18:00:07.22 Forearm.seek :garage @18:00:08.24 Hand.close @18:00:09.02 Forearm.seek :retract @18:00:10.01 Elbow.reconfigure :tight @18:00:10.06 Elbow.seek PC @18:00:11.33 Elbow.reconfigure :loose @18:00:11.43 Forearm.reconfigure :tight @18:00:11.48 Forearm.seek PC @18:00:12.67 PC.seek :closed @18:00:17.32 Processing Clamp onSpring/offSpring for 16/26 samples, on/off torque 1.61 @18:00:17.83 Hand.open @18:00:18.46 Forearm.reconfigure :loose @18:00:18.51 Forearm.seek :retract @18:00:19.49 PRV.dial :air @18:00:19.94 PSV.dial :mixloop @18:00:20.48 PS.seek 0.3ml @18:00:24.50 PSV.dial PSR @18:00:25.68 Pulling 0.4ml of PSR.air @18:00:25.69 PSR.select :air @18:00:25.71 PS.seek 0.7ml @18:00:30.59 delay 3 seconds @18:00:33.61 PSR.select :air @18:00:33.63 PS.seek 2.5ml @18:00:52.81 delay 3 seconds @18:00:55.83 PSV.dial :mixloop @18:00:56.98 PTV.dial :mixing @18:00:57.73 PBV.dial :waste2 @18:00:58.69 PS.seek 0ml @18:01:25.03 Garaging FlushPuck in Processing Clamp @18:01:25.10 Elbow.reconfigure :tight @18:01:25.15 Elbow.seek PC @18:01:25.27 Elbow.reconfigure :loose @18:01:25.37 Forearm.seek PC @18:01:26.32 Hand.close @18:01:26.90 PC.seek :open @18:01:31.61 Forearm.seek :retract @18:01:32.59 Elbow.reconfigure :tight @18:01:32.63 Elbow.seek :garage @18:01:34.01 Elbow.reconfigure :loose @18:01:34.11 Forearm.reconfigure :tight @18:01:34.17 Forearm.seek :garage @18:01:35.21 Hand.open @18:01:35.83 Forearm.reconfigure :loose @18:01:35.88 Forearm.seek :retract @18:01:36.88 Loading da puck from tube 3 into Processing Clamp @18:01:37.11 SC.reconfigure :tight @18:01:37.16 SC.seek 3 @18:01:40.66 SC.configure :limp @18:01:41.79 Elbow.reconfigure :tight @18:01:41.84 Elbow.seek 3 @18:01:43.71 Forearm.reconfigure :tight @18:01:43.76 Forearm.seek 3 + 450 counts @18:01:44.79 SE.reconfigure :pick @18:01:44.84 SE.seek :beamBreak @18:01:45.82 SE.configure :default @18:01:45.98 Elbow.seek 3 @18:01:46.16 Forearm.seek 3 + 300 counts @18:01:46.41 Forearm.reconfigure :loose @18:01:46.46 Hand.close @18:01:47.04 SE.seek :down @18:01:47.26
Forearm.seek :retract @18:01:47.73 SE.stop @18:01:48.21
Elbow.seek PC @18:01:50.73 Elbow.reconfigure :loose @18:01:50.84 Forearm.reconfigure :tight @18:01:50.88 Forearm.seek PC @18:01:52.16 PC.seek :closed @18:01:56.78 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.64 @18:01:56.79 Hand.open @18:01:57.45 Forearm.reconfigure :loose @18:01:57.50 Forearm.seek :retract @18:01:58.48 Heating Puck.from 3, :da in Processing Clamp to 28C for 2:00:00 @18:01:58.88 PH.seek 28C,5:00 @18:02:38.05 PH.hold 2:00:00,28C @18:02:38.10 delay 1:30 @18:04:08.14 PSR.select :air @18:04:08.16 PSV.dial PSR @18:04:09.20 Pulling 0.5ml of PSR.wash PSR.select :wash @18:04:09.32 PS.seek 0.5ml @18:04:15.28 delay 5 seconds @18:04:20.29 PSR.select :air @18:04:20.42 PS.seek 2ml @18:04:36.52 PTV.dial :puck @18:04:37.27 PSV.dial PTV @18:04:38.04 PBV.dial :waste1 @18:04:39.43 PS.seek 1.5ml @18:04:45.34 delay 3 seconds @18:04:48.36 PBV.dial between PSV and waste1 @18:04:48.70 PS.seek 0ml @18:05:05.27 delay 5:00 @18:10:05.30 recoverPSRreagent08 @18:10:05.31 PTV.dial :puck @18:10:05.38 PBV.dial :waste1 @18:10:05.74 delay 30 seconds @18:10:35.76 PBV.dial between PSV and waste1 @18:10:36.13 PSR.select :air @18:10:36.14 PSV.dial PSR @18:10:36.90 PS.reconfigure :fast @18:10:36.95 PS.seek 2.5ml @18:11:00.80 PSV.dial PTV @18:11:01.67 delay 5 seconds @18:11:06.69 PSV.dial PSR @18:11:07.42 delay 5 seconds @18:11:12.44 PSV.dial PTV @18:11:13.19 delay 5 seconds @18:11:18.21 PSV.dial :air @18:11:18.97 PS.seek 0ml @18:11:42.30 PTV.dial :PRVmixing,via: PRV @18:11:43.62 PSV.dial :mixloop @18:11:44.09 PRV.dial :ab1 @18:11:44.85 PS.reconfigure :slow @18:11:44.90 PS.seek 0.15ml @18:11:48.45 delay 2 seconds @18:11:50.47 CBV.dial PRV @18:11:50.50
PRV.dial CBV @18:11:51.07 PS.seek 0.35ml @18:11:55.61 delay 2 seconds @18:11:57.63 CBV.dial between PRV and PSV @18:11:57.95 PRV.dial :air @18:11:58.41 PS.seek 0.9ml @18:12:10.14 PRV.dial :waste2 @18:12:10.88 PS.seek 0.5ml @18:12:19.51 addPMLReagent08 @18:12:19.52 PSV.dial :air @18:12:19.99 PS.reconfigure :default @18:12:20.04 PS.seek 2ml @18:12:36.36 PSV.dial :PML @18:12:36.83 PTV.dial :mixing @18:12:37.58 PBV.dial :waste1 @18:12:38.00 PS.seek 1.85ml @18:12:40.36 PBV.dial between PSV and waste1 @18:12:40.72 PS.seek 0ml @18:13:00.48 delay 2 seconds @18:13:02.50 Beginning samplePassC @18:13:02.51 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @18:13:02.70 Elbow.reconfigure :tight @18:13:02.75 Elbow.seek FlushPuck::Garage @18:13:04.54 Elbow.reconfigure :loose @18:13:04.65 Forearm.seek :garage @18:13:05.66 Hand.close @18:13:06.39 Forearm.seek :retract @18:13:07.36 Elbow.reconfigure :tight @18:13:07.41 Elbow.seek CC @18:13:10.43 Elbow.reconfigure :loose @18:13:10.53 Forearm.reconfigure :tight @18:13:10.58 Forearm.seek CC @18:13:11.85 CC.seek :closed @18:13:16.42 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.30 @18:13:16.49 Hand.open @18:13:17.20 Forearm.reconfigure :loose @18:13:17.25 Forearm.seek :retract @18:13:18.23 PSV.dial PSR @18:13:19.41 Pulling 1.5ml of PSR.flush PSR.select :flush @18:13:19.53 PS.seek 1.5ml @18:13:35.73 delay 5 seconds @18:13:40.75 PSV.dial :waste1 @18:13:41.22 PS.seek 0ml @18:13:57.42 PSV.dial PSR @18:13:57.89 PSR.select :air @18:13:58.00 PS.seek 2.5ml @18:14:24.39 PRV.dial CBV,avoiding: :ab2 @18:14:24.42
CBV.dial PRV @18:14:26.03 PSV.dial :mixloop @18:14:27.51 CTV.dial :air,avoiding: :intake @18:14:27.68 PTV.dial :PRVmixing @18:14:28.47 PS.seek 0ml @18:14:54.85 PSV.dial :air @18:14:55.33 PS.seek 2.5ml @18:15:21.63 PSV.dial :mixloop @18:15:22.11 PS.seek 0ml @18:15:48.51 PSV.dial :air @18:15:48.99 PS.seek 2.5ml @18:16:15.38 PSV.dial :mixloop @18:16:15.86 PS.seek 0ml @18:16:42.20 Garaging FlushPuck in Collection Clamp @18:16:42.27 Elbow.reconfigure :tight @18:16:42.32 Elbow.seek CC @18:16:42.66 Elbow.reconfigure :loose @18:16:42.76 Forearm.seek CC @18:16:43.81 Hand.close @18:16:44.39 CC.seek :open @18:16:49.00 Forearm.seek :retract @18:16:50.00 Elbow.reconfigure :tight @18:16:50.05 Elbow.seek :garage @18:16:52.96 Elbow.reconfigure :loose @18:16:53.06 Forearm.reconfigure :tight @18:16:53.11 Forearm.seek :garage @18:16:54.12 Hand.open @18:16:54.82 Forearm.reconfigure :loose @18:16:54.87 Forearm.seek :retract @18:16:55.86 delayUntil "18:21:02.49" @18:16:55.88 Whole Cell Archive sampling 1000ml @18:16:55.90 Loading wcr puck from tube 3 into Collection Clamp @18:16:56.15 SC.reconfigure :tight @18:16:56.20 SC.seek 3 @18:16:56.30 SC.configure :limp @18:16:56.97 Elbow.reconfigure :tight @18:16:57.02 Elbow.seek 3 @18:16:59.12 Forearm.reconfigure :tight @18:16:59.18 Forearm.seek 3 + 450 counts @18:17:00.28 SE.reconfigure :pick @18:17:00.34 SE.seek :beamBreak @18:17:01.42 SE.configure :default @18:17:01.58 Elbow.seek 3 @18:17:01.78 Forearm.seek 3 + 300 counts @18:17:01.93 Forearm.reconfigure :loose @18:17:01.98 Hand.close @18:17:02.65 SE.seek :down @18:17:02.86 Forearm.seek :retract @18:17:03.42 SE.stop @18:17:03.79 Elbow.seek CC @18:17:05.44 Elbow.reconfigure :loose @18:17:05.55 Forearm.reconfigure :tight @18:17:05.60 Forearm.seek CC @18:17:06.90 CC.seek :closed @18:17:11.42 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.26 @18:17:11.88 Hand.open @18:17:12.57 Forearm.reconfigure :loose @18:17:12.62 Forearm.seek :retract @18:17:13.62 collectSample 1000ml w/bubble point of 35psi @18:17:13.64 Priming 7ml @18:17:13.65 Intake.select :close @18:17:13.66 Exhaust.select :close @18:17:13.67 SS.hold @18:17:13.89 Calibrating pressure sensors @18:17:13.90 CTV.dial :air @18:17:13.91 SSV.dial :spit,avoiding: :exhaust @18:17:13.99 SS.reconfigure :fast @18:17:14.05 SS.seek 5ml @18:17:16.72 SSV.dial CBV @18:17:16.75 CBV.dial SSV @18:17:18.13 delay 3.8 seconds @18:17:22.07 Sensor limits: Intake max 112.8psia; Outlet max 104.7psia @18:17:22.08 SSV.dial :spit @18:17:23.42 SS.seek 0ml @18:17:26.47 SSV.dial CBV @18:17:27.80 CTV.dial :intake @18:17:28.59 SSV.dial CBV @18:17:28.63 CBV.dial SSV @18:17:28.77 Allowing 40s for 7.0ml of remaining 7.0ml @18:17:28.78 Intake.select :open @18:17:30.94 Intake @14.4psia, vacuum from 8.9 to 11.9psi @18:17:30.96 SS.reconfigure :inhaling 2.20ml/s @18:17:31.01 SS.seek 7ml @18:17:36.76 Stroke completed -- waiting for pressure to equalize ... @18:17:36.78 SS.reconfigure :exhale @18:17:36.82 SS.hold @18:17:49.49 Intake.select :close @18:17:49.65 SSV.dial :exhaust @18:17:50.42 Exhaust.select :open @18:17:50.54 SS.seek 0ml @18:17:54.86 Exhaust.select :close @18:17:54.97 Sampling 1000.0ml @18:17:54.98 Intake.select :close @18:17:54.99 Exhaust.select :close @18:17:55.01 SS.reconfigure :default @18:17:55.05 SS.hold @18:17:55.26 CTV.dial :intake @18:17:55.48 SSV.dial CBV @18:17:55.52 CBV.dial SSV @18:17:56.27 Allowing 130s for 25.0ml of remaining 1000.0ml @18:17:56.28 Intake.select :open @18:17:58.45 Intake @15.1psia, vacuum from 9.6 to 12.6psi @18:17:58.47 SS.reconfigure :inhaling 2.20ml/s @18:17:58.52 SS.seek 25ml @18:18:11.05 Vacuum too high -- waiting up to 40 seconds @18:18:11.07 SS.reconfigure :exhale @18:18:11.12 SS.hold @18:18:21.47 Intake @15.4psia, vacuum from 9.9 to 12.9psi @18:18:21.49 SS.reconfigure :inhaling 1.76ml/s @18:18:21.54 SS.seek 25ml @18:18:23.90 Stroke completed -- waiting for pressure to equalize ... @18:18:23.92 SS.reconfigure :exhale @18:18:23.97 SS.hold @18:18:37.11 Intake.select :close @18:18:37.27 SSV.dial :exhaust @18:18:38.05 Exhaust.select :open @18:18:38.16 SS.seek 0ml @18:18:46.94 Exhaust.select :close @18:18:47.05 SSV.dial CBV @18:18:47.08 CBV.dial SSV @18:18:47.85 Allowing 130s for 25.0ml of remaining 975.0ml @18:18:47.86 Intake.select :open @18:18:50.02 Intake @15.5psia, vacuum from 10.0 to 13.0psi @18:18:50.04 SS.reconfigure :inhaling 1.76ml/s @18:18:50.09 SS.seek 25ml @18:19:07.54 Stroke completed -- waiting for pressure to equalize ... @18:19:07.56 SS.reconfigure :exhale @18:19:07.60 SS.hold @18:19:32.21 Intake.select :close @18:19:32.37 SSV.dial :exhaust @18:19:33.14 Exhaust.select :open @18:19:33.25 SS.seek 0ml @18:19:42.06 Exhaust.select :close @18:19:42.17 SSV.dial CBV @18:19:42.20 CBV.dial SSV @18:19:42.97 Allowing 130s for 25.0ml of remaining 950.0ml @18:19:42.98 Intake.select :open @18:19:45.15 Intake @15.6psia, vacuum from 10.0 to 13.0psi @18:19:45.17 SS.reconfigure :inhaling 1.76ml/s @18:19:45.22 SS.seek 25ml @18:20:02.65 Stroke completed -- waiting for pressure to equalize ... @18:20:02.67 SS.reconfigure :exhale @18:20:02.71 SS.hold @18:20:21.10 Intake.select :close @18:20:21.26 SSV.dial :exhaust @18:20:22.03 Exhaust.select :open @18:20:22.14 SS.seek 0ml @18:20:30.93 Exhaust.select :close @18:20:31.05 SSV.dial CBV @18:20:31.09 CBV.dial SSV @18:20:31.84 Allowing 130s for 25.0ml of remaining 925.0ml @18:20:31.86 Intake.select :open @18:20:34.02 Intake @15.7psia, vacuum from 10.0 to 13.0psi @18:20:34.04 SS.reconfigure :inhaling 1.76ml/s @18:20:34.09 SS.seek 25ml @18:20:50.89 Vacuum too high -- waiting up to 40 seconds @18:20:50.91 SS.reconfigure :exhale @18:20:50.96 SS.hold @18:20:59.08 Intake @15.8psia, vacuum from 10.0 to 13.0psi @18:20:59.10 SS.reconfigure :inhaling 1.41ml/s @18:20:59.15 SS.seek 25ml @18:20:59.78 Stroke completed -- waiting for pressure to equalize ... @18:20:59.79 SS.reconfigure :exhale @18:20:59.84 SS.hold @18:21:02.50 recoverPRVreagent08 @18:21:02.51 PTV.dial :mixing @18:21:03.27 PBV.dial :waste1 @18:21:03.64 delay 30 seconds @18:21:11.49 Intake.select :close @18:21:11.65 SSV.dial :exhaust @18:21:12.42 Exhaust.select :open @18:21:12.53 SS.seek 0ml @18:21:21.23 Exhaust.select :close @18:21:21.35 SSV.dial CBV @18:21:21.38 CBV.dial SSV @18:21:22.14 Allowing 130s for 25.0ml of remaining 900.0ml @18:21:22.15 Intake.select :open @18:21:24.32 Intake @15.6psia, vacuum from 10.0 to 13.0psi @18:21:24.34 SS.reconfigure :inhaling 1.41ml/s @18:21:24.39 SS.seek 25ml @18:21:33.67 PBV.dial between PSV and waste1 @18:21:34.04 PSV.dial :air @18:21:34.51 PS.reconfigure :fast @18:21:34.56 PS.seek 2.5ml @18:21:44.93 Stroke completed -- waiting for pressure to equalize ... @18:21:44.95 SS.reconfigure :exhale @18:21:44.99 SS.hold @18:21:58.63 PSV.dial :mixloop @18:21:59.10 delay 2 seconds @18:22:01.12 PSV.dial :air @18:22:01.60 delay 2 seconds @18:22:03.39 Intake.select :close @18:22:03.54 SSV.dial :exhaust @18:22:03.61 PSV.dial :mixloop @18:22:04.08 delay 2 seconds @18:22:04.31 Exhaust.select :open @18:22:04.42 SS.seek 0ml @18:22:06.10 PSV.dial :air @18:22:06.57 PS.seek 0ml @18:22:13.20 Exhaust.select :close @18:22:13.31 SSV.dial CBV @18:22:13.35 CBV.dial SSV @18:22:14.11 Allowing 130s for 25.0ml of remaining 875.0ml @18:22:14.12 Intake.select :open @18:22:16.28 Intake @15.6psia, vacuum from 10.0 to 13.0psi @18:22:16.30 SS.reconfigure :inhaling 1.41ml/s @18:22:16.35 SS.seek 25ml @18:22:30.41 washCycleDA #1 of 2 @18:22:30.42 PSR.select :air @18:22:30.44 PSV.dial PSR @18:22:31.78 Pulling 0.5ml of PSR.wash @18:22:31.79 PSR.select :wash @18:22:31.91 PS.reconfigure :default @18:22:31.96 PS.seek 0.5ml @18:22:36.96 Stroke completed -- waiting for pressure to equalize ... @18:22:36.98 SS.reconfigure :exhale @18:22:37.02 SS.hold @18:22:38.02 delay 5 seconds @18:22:43.04 PSR.select :air @18:22:43.16 PS.seek 2ml @18:22:53.40 Intake.select :close @18:22:53.56 SSV.dial :exhaust @18:22:54.32 Exhaust.select :open @18:22:54.44 SS.seek 0ml @18:22:59.38 PTV.dial :puck @18:23:00.13 PSV.dial PTV @18:23:00.88 PBV.dial :waste1 @18:23:01.31 PS.seek 1.5ml @18:23:03.51 Exhaust.select :close @18:23:03.62 SSV.dial CBV @18:23:03.65 CBV.dial SSV @18:23:04.41 Allowing 130s for 25.0ml of remaining 850.0ml @18:23:04.42 Intake.select :open @18:23:06.58 Intake @15.7psia, vacuum from 10.0 to 13.0psi @18:23:06.60 SS.reconfigure :inhaling 1.41ml/s @18:23:06.65 SS.seek 25ml @18:23:07.28 delay 3 seconds @18:23:10.30 PBV.dial between PSV and waste1 @18:23:10.68 PS.seek 0ml @18:23:26.79 Stroke completed -- waiting for pressure to equalize ... @18:23:26.80 SS.reconfigure :exhale @18:23:26.85 SS.hold @18:23:27.06 delay 2:00 @18:23:43.46 Intake.select :close @18:23:43.62 SSV.dial :exhaust @18:23:44.42 Exhaust.select :open @18:23:44.60 SS.seek 0ml @18:23:53.62 Exhaust.select :close @18:23:53.73 SSV.dial CBV @18:23:53.76 CBV.dial SSV @18:23:54.53 Allowing 130s for 25.0ml of remaining 825.0ml @18:23:54.54 Intake.select :open @18:23:56.71 Intake @15.6psia, vacuum from 10.0 to 13.0psi @18:23:56.73 SS.reconfigure :inhaling 1.41ml/s @18:23:56.78 SS.seek 25ml @18:24:16.98 Stroke completed -- waiting for pressure to equalize ... @18:24:16.99 SS.reconfigure :exhale @18:24:17.03 SS.hold @18:24:33.85 Intake.select :close @18:24:34.01 SSV.dial :exhaust @18:24:34.77 Exhaust.select :open @18:24:34.89 SS.seek 0ml @18:24:43.59 Exhaust.select :close @18:24:43.70 SSV.dial CBV @18:24:43.72 CBV.dial SSV @18:24:44.49 Allowing 130s for 25.0ml of remaining 800.0ml @18:24:44.51 Intake.select :open @18:24:46.67 Intake @15.6psia, vacuum from 10.0 to 13.0psi @18:24:46.69 SS.reconfigure :inhaling 1.41ml/s @18:24:46.74 SS.seek 25ml @18:25:07.46 Stroke completed -- waiting for pressure to equalize ... @18:25:07.48 SS.reconfigure :exhale @18:25:07.52 SS.hold @18:25:23.75 Intake.select :close @18:25:23.91 SSV.dial :exhaust @18:25:24.68 Exhaust.select :open @18:25:24.79 SS.seek 0ml @18:25:27.08 recoverPSRreagent08 @18:25:27.10 PTV.dial :puck @18:25:27.17 PBV.dial :waste1 @18:25:27.55 delay 30 seconds @18:25:33.80 Exhaust.select :close @18:25:33.92 SSV.dial CBV @18:25:33.96 CBV.dial SSV @18:25:35.18 Allowing 130s for 25.0ml of remaining 775.0ml @18:25:35.20 Intake.select :open @18:25:37.37 Intake @15.5psia, vacuum from 10.0 to 13.0psi @18:25:37.39 SS.reconfigure :inhaling 1.41ml/s @18:25:37.44 SS.seek 25ml @18:25:57.43 Stroke completed -- waiting for pressure to equalize ... @18:25:57.44 SS.reconfigure :exhale @18:25:57.49 SS.hold @18:25:57.57 PBV.dial between PSV and waste1 @18:25:57.93 PSR.select :air @18:25:57.95 PSV.dial PSR @18:25:58.78 PS.reconfigure :fast @18:25:58.83 PS.seek 2.5ml @18:26:17.99 Intake.select :close @18:26:18.16 SSV.dial :exhaust @18:26:18.94 Exhaust.select :open @18:26:19.05 SS.seek 0ml @18:26:23.03 PSV.dial PTV @18:26:23.80 delay 5 seconds @18:26:27.79 Exhaust.select :close @18:26:27.90 SSV.dial CBV @18:26:27.93 CBV.dial SSV @18:26:28.70 Allowing 130s for 25.0ml of remaining 750.0ml @18:26:28.71 Intake.select :open @18:26:28.82 PSV.dial PSR @18:26:29.59 delay 5 seconds @18:26:30.87 Intake @15.5psia, vacuum from 10.0 to 13.0psi @18:26:30.89 SS.reconfigure :inhaling 1.41ml/s @18:26:30.94 SS.seek 25ml @18:26:34.61 PSV.dial PTV @18:26:35.52 delay 5 seconds @18:26:40.54 PSV.dial :air @18:26:41.42 PS.seek 0ml @18:26:48.27 Vacuum too high -- waiting up to 40 seconds @18:26:48.28 SS.reconfigure :exhale @18:26:48.34 SS.hold @18:26:59.84 Intake @16.1psia, vacuum from 10.0 to 13.0psi @18:26:59.86 SS.reconfigure :inhaling 1.13ml/s @18:26:59.91 SS.seek 25ml @18:27:04.25 Stroke completed -- waiting for pressure to equalize ... @18:27:04.27 SS.reconfigure :exhale @18:27:04.32 SS.hold @18:27:05.26 washCycleDA #2 of 2 @18:27:05.27 PSR.select :air @18:27:05.29 PSV.dial PSR @18:27:06.64 Pulling 0.5ml of PSR.wash @18:27:06.65 PSR.select :wash @18:27:06.81 PS.reconfigure :default @18:27:06.92 PS.seek 0.5ml @18:27:12.96 delay 5 seconds @18:27:12.99 Intake.select :close @18:27:13.15 SSV.dial :exhaust @18:27:13.91 Exhaust.select :open @18:27:14.03 SS.seek 0ml @18:27:17.99 PSR.select :air @18:27:18.11 PS.seek 2ml @18:27:23.03 Exhaust.select :close @18:27:23.14 SSV.dial CBV @18:27:23.17 CBV.dial SSV @18:27:23.94 Allowing 130s for 25.0ml of remaining 725.0ml @18:27:23.96 Intake.select :open @18:27:26.12 Intake @15.5psia, vacuum from 10.0 to 13.0psi @18:27:26.14 SS.reconfigure :inhaling 1.13ml/s @18:27:26.19 SS.seek 25ml @18:27:34.87 PTV.dial :puck @18:27:34.95 PSV.dial PTV @18:27:35.72 PBV.dial :waste1 @18:27:36.14 PS.seek 1.5ml @18:27:42.09 delay 3 seconds @18:27:45.11 PBV.dial between PSV and waste1 @18:27:45.47 PS.seek 0ml @18:27:49.39 Vacuum too high -- waiting up to 40 seconds @18:27:49.40 SS.reconfigure :exhale @18:27:49.46 SS.hold @18:28:01.19 Intake @15.6psia, vacuum from 10.0 to 13.0psi @18:28:01.21 SS.reconfigure :inhaling 0.91ml/s @18:28:01.26 SS.seek 25ml @18:28:01.59 delay 2:00 @18:28:02.92 Stroke completed -- waiting for pressure to equalize ... @18:28:02.94 SS.reconfigure :exhale @18:28:02.99 SS.hold @18:28:10.39 Intake.select :close @18:28:10.55 SSV.dial :exhaust @18:28:11.32 Exhaust.select :open @18:28:11.44 SS.seek 0ml @18:28:20.39 Exhaust.select :close @18:28:20.51 SSV.dial CBV @18:28:20.55 CBV.dial SSV @18:28:21.30 Allowing 130s for 25.0ml of remaining 700.0ml @18:28:21.32 Intake.select :open @18:28:23.49 Intake @15.4psia, vacuum from 9.9 to 12.9psi @18:28:23.51 SS.reconfigure :inhaling 0.91ml/s @18:28:23.56 SS.seek 25ml @18:28:53.08 Stroke completed -- waiting for pressure to equalize ... @18:28:53.09 SS.reconfigure :exhale @18:28:53.14 SS.hold @18:29:11.79 Intake.select :close @18:29:11.95 SSV.dial :exhaust @18:29:12.72 Exhaust.select :open @18:29:12.84 SS.seek 0ml @18:29:21.95 Exhaust.select :close @18:29:22.07 SSV.dial CBV @18:29:22.11 CBV.dial SSV @18:29:22.86 Allowing 130s for 25.0ml of remaining 675.0ml @18:29:22.88 Intake.select :open @18:29:25.05 Intake @15.4psia, vacuum from 9.9 to 12.9psi @18:29:25.07 SS.reconfigure :inhaling 0.91ml/s @18:29:25.12 SS.seek 25ml @18:29:54.41 Stroke completed -- waiting for pressure to equalize ... @18:29:54.42 SS.reconfigure :exhale @18:29:54.85 SS.hold @18:30:01.61 recoverPSRreagent08 @18:30:01.62 PTV.dial :puck @18:30:01.70 PBV.dial :waste1 @18:30:02.07 delay 30 seconds @18:30:15.34 Intake.select :close @18:30:15.49 SSV.dial :exhaust @18:30:16.26 Exhaust.select :open @18:30:16.37 SS.seek 0ml @18:30:25.78 Exhaust.select :close @18:30:25.96 SSV.dial CBV @18:30:25.99 CBV.dial SSV @18:30:26.77 Allowing 130s for 25.0ml of remaining 650.0ml @18:30:26.78 Intake.select :open @18:30:28.95 Intake @15.3psia, vacuum from 9.8 to 12.8psi @18:30:28.97 SS.reconfigure :inhaling 0.91ml/s @18:30:29.02 SS.seek 25ml @18:30:32.09 PBV.dial between PSV and waste1 @18:30:32.46 PSR.select :air @18:30:32.48 PSV.dial PSR @18:30:33.25 PS.reconfigure :fast @18:30:33.30 PS.seek 2.5ml @18:30:51.10 Vacuum too high -- waiting up to 40 seconds @18:30:51.12 SS.reconfigure :exhale @18:30:51.17 SS.hold @18:30:57.33 PSV.dial PTV @18:30:58.10 delay 5 seconds @18:31:01.14 Intake @15.3psia, vacuum from 9.8 to 12.8psi @18:31:01.16 SS.reconfigure :inhaling 0.73ml/s @18:31:01.20 SS.seek 25ml @18:31:03.12 PSV.dial PSR @18:31:03.88 delay 5 seconds @18:31:08.90 PSV.dial PTV @18:31:10.12 delay 5 seconds @18:31:10.87 Stroke completed -- waiting for pressure to equalize ... @18:31:10.89 SS.reconfigure :exhale @18:31:10.94 SS.hold @18:31:15.13 PSV.dial :air @18:31:15.90 PS.seek 0ml @18:31:25.22 Intake.select :close @18:31:25.38 SSV.dial :exhaust @18:31:26.14 Exhaust.select :open @18:31:26.26 SS.seek 0ml @18:31:35.28 Exhaust.select :close @18:31:35.39 SSV.dial CBV @18:31:35.43 CBV.dial SSV @18:31:36.17 Allowing 130s for 25.0ml of remaining 625.0ml @18:31:36.19 Intake.select :open @18:31:38.76 Intake @15.3psia, vacuum from 9.8 to 12.8psi @18:31:38.78 SS.reconfigure :inhaling 0.73ml/s @18:31:38.83 SS.seek 25ml @18:31:39.59 getPMLReagent08 @18:31:39.60 PRV.dial :air @18:31:40.07 delay 5 seconds @18:31:45.09 PTV.dial :PRVmixing @18:31:46.43 delay 5 seconds @18:31:51.45 PRV.dial :ab2 @18:31:51.90 PTV.dial :PRVmixing @18:31:52.09 PSV.dial :mixloop @18:31:52.56 PS.reconfigure :default @18:31:52.61 PS.seek 0.3ml @18:31:56.53 delay 2 seconds @18:31:58.55 PRV.dial :air @18:31:59.07 PS.seek 0.55ml @18:32:02.43 addPMLReagent08 @18:32:02.44 PSV.dial :air @18:32:02.92 PS.seek 2ml @18:32:14.23 Vacuum too high -- waiting up to 40 seconds @18:32:14.24 SS.reconfigure :exhale @18:32:14.30 SS.hold @18:32:18.58 PSV.dial :PML @18:32:19.05 PTV.dial :mixing @18:32:19.82 PBV.dial :waste1 @18:32:20.25 PS.seek 1.85ml @18:32:22.61 PBV.dial between PSV and waste1 @18:32:23.45 PS.seek 0ml @18:32:27.43 Intake @15.4psia, vacuum from 9.9 to 12.9psi @18:32:27.45 SS.reconfigure :inhaling 0.59ml/s @18:32:27.50 SS.seek 25ml @18:32:28.70 Stroke completed -- waiting for pressure to equalize ... @18:32:28.71 SS.reconfigure :exhale @18:32:28.76 SS.hold @18:32:38.54 Intake.select :close @18:32:38.71 SSV.dial :exhaust @18:32:39.47 Exhaust.select :open @18:32:39.60 SS.seek 0ml @18:32:43.12 delay 2 seconds @18:32:45.54 delay 8:00 @18:32:48.34 Exhaust.select :close @18:32:48.46 SSV.dial CBV @18:32:48.49 CBV.dial SSV @18:32:49.27 Allowing 130s for 25.0ml of remaining 600.0ml @18:32:49.28 Intake.select :open @18:32:51.44 Intake @15.3psia, vacuum from 9.8 to 12.8psi @18:32:51.46 SS.reconfigure :inhaling 0.59ml/s @18:32:51.51 SS.seek 25ml @18:33:36.23 Stroke completed -- waiting for pressure to equalize ... @18:33:36.24 SS.reconfigure :exhale @18:33:36.29 SS.hold @18:33:58.83 Intake.select :close @18:33:58.99 SSV.dial :exhaust @18:33:59.76 Exhaust.select :open @18:33:59.88 SS.seek 0ml @18:34:08.68 Exhaust.select :close @18:34:08.80 SSV.dial CBV @18:34:08.84 CBV.dial SSV @18:34:10.34 Allowing 130s for 25.0ml of remaining 575.0ml @18:34:10.36 Intake.select :open @18:34:12.52 Intake @15.2psia, vacuum from 9.7 to 12.7psi @18:34:12.54 SS.reconfigure :inhaling 0.59ml/s @18:34:12.59 SS.seek 25ml @18:34:52.97 Vacuum too high -- waiting up to 40 seconds @18:34:52.99 SS.reconfigure :exhale @18:34:53.04 SS.hold @18:35:04.64 Intake @15.1psia, vacuum from 9.6 to 12.6psi @18:35:04.72 SS.reconfigure :inhaling 0.47ml/s @18:35:04.77 SS.seek 25ml @18:35:10.68 Stroke completed -- waiting for pressure to equalize ... @18:35:10.69 SS.reconfigure :exhale @18:35:10.74 SS.hold @18:35:25.13 Intake.select :close @18:35:25.29 SSV.dial :exhaust @18:35:26.06 Exhaust.select :open @18:35:26.17 SS.seek 0ml @18:35:34.96 Exhaust.select :close @18:35:35.08 SSV.dial CBV @18:35:35.12 CBV.dial SSV @18:35:35.87 Allowing 130s for 25.0ml of remaining 550.0ml @18:35:35.88 Intake.select :open @18:35:38.52 Intake @15.3psia, vacuum from 9.8 to 12.8psi @18:35:38.54 SS.reconfigure :inhaling 0.47ml/s @18:35:38.59 SS.seek 25ml @18:36:34.69 Stroke completed -- waiting for pressure to equalize ... @18:36:34.71 SS.reconfigure :exhale @18:36:34.76 SS.hold @18:36:59.26 Intake.select :close @18:36:59.84 SSV.dial :exhaust @18:37:00.60 Exhaust.select :open @18:37:00.72 SS.seek 0ml @18:37:09.54 Exhaust.select :close @18:37:09.65 SSV.dial CBV @18:37:09.68 CBV.dial SSV @18:37:10.45 Allowing 130s for 25.0ml of remaining 525.0ml @18:37:10.46 Intake.select :open @18:37:12.63 Intake @15.3psia, vacuum from 9.8 to 12.8psi @18:37:12.65 SS.reconfigure :inhaling 0.47ml/s @18:37:12.71 SS.seek 25ml @18:38:08.52 Stroke completed -- waiting for pressure to equalize ... @18:38:08.53 SS.reconfigure :exhale @18:38:08.58 SS.hold @18:38:39.73 Intake.select :close @18:38:39.89 SSV.dial :exhaust @18:38:40.66 Exhaust.select :open @18:38:40.78 SS.seek 0ml @18:38:49.80 Exhaust.select :close @18:38:49.92 SSV.dial CBV @18:38:49.94 CBV.dial SSV @18:38:50.71 Allowing 130s for 25.0ml of remaining 500.0ml @18:38:50.72 Intake.select :open @18:38:52.90 Intake @15.3psia, vacuum from 9.8 to 12.8psi @18:38:52.92 SS.reconfigure :inhaling 0.47ml/s @18:38:52.97 SS.seek 25ml @18:39:48.43 Stroke completed -- waiting for pressure to equalize ... @18:39:48.44 SS.reconfigure :exhale @18:39:48.49 SS.hold @18:40:25.41 Intake.select :close @18:40:25.57 SSV.dial :exhaust @18:40:26.33 Exhaust.select :open @18:40:26.45 SS.seek 0ml @18:40:35.27 Exhaust.select :close @18:40:35.38 SSV.dial CBV @18:40:35.41 CBV.dial SSV @18:40:36.18 Allowing 130s for 25.0ml of remaining 475.0ml @18:40:36.19 Intake.select :open @18:40:38.35 Intake @15.3psia, vacuum from 9.8 to 12.8psi @18:40:38.37 SS.reconfigure :inhaling 0.47ml/s @18:40:38.42 SS.seek 25ml @18:40:45.62 recoverPRVreagent08 @18:40:45.64 PTV.dial :mixing @18:40:45.71 PBV.dial :waste1 @18:40:46.25 delay 30 seconds @18:41:16.27 PBV.dial between PSV and waste1 @18:41:16.65 PSV.dial :air @18:41:17.27 PS.reconfigure :fast @18:41:17.32 PS.seek 2.5ml @18:41:27.50 Vacuum too high -- waiting up to 40 seconds @18:41:27.51 SS.reconfigure :exhale @18:41:27.57 SS.hold @18:41:41.51 PSV.dial :mixloop @18:41:41.96 delay 2 seconds @18:41:44.05 PSV.dial :air @18:41:44.64 delay 2 seconds @18:41:46.65 PSV.dial :mixloop @18:41:47.11 delay 2 seconds @18:41:48.67 Intake @15.7psia, vacuum from 10.0 to 13.0psi @18:41:48.69 SS.reconfigure :inhaling 0.38ml/s @18:41:48.74 SS.seek 25ml @18:41:49.12 PSV.dial :air @18:41:49.60 PS.seek 0ml @18:41:57.49 Stroke completed -- waiting for pressure to equalize ... @18:41:57.50 SS.reconfigure :exhale @18:41:57.55 SS.hold @18:42:13.11 washCycleDA #1 of 3 @18:42:13.12 PSR.select :air @18:42:13.13 PSV.dial PSR @18:42:14.46 Pulling 0.5ml of PSR.wash @18:42:14.47 PSR.select :wash @18:42:14.59 PS.reconfigure :default @18:42:14.64 PS.seek 0.5ml @18:42:18.45 Intake.select :close @18:42:18.61 SSV.dial :exhaust @18:42:19.38 Exhaust.select :open @18:42:19.49 SS.seek 0ml @18:42:20.51 delay 5 seconds @18:42:25.53 PSR.select :air @18:42:25.64 PS.seek 2ml @18:42:28.30 Exhaust.select :close @18:42:28.41 SSV.dial CBV @18:42:28.45 CBV.dial SSV @18:42:29.21 Allowing 130s for 25.0ml of remaining 450.0ml @18:42:29.22 Intake.select :open @18:42:31.39 Intake @15.2psia, vacuum from 9.7 to 12.7psi @18:42:31.41 SS.reconfigure :inhaling 0.38ml/s @18:42:31.46 SS.seek 25ml @18:42:41.73 PTV.dial :puck @18:42:42.48 PSV.dial PTV @18:42:43.23 PBV.dial :waste1 @18:42:43.67 PS.seek 1.5ml @18:42:49.70 delay 3 seconds @18:42:52.72 PBV.dial between PSV and waste1 @18:42:53.10 PS.seek 0ml @18:43:09.32 delay 2:00 @18:43:40.73 Stroke completed -- waiting for pressure to equalize ... @18:43:40.75 SS.reconfigure :exhale @18:43:40.79 SS.hold @18:44:20.16 Intake.select :close @18:44:20.31 SSV.dial :exhaust @18:44:21.08 Exhaust.select :open @18:44:21.19 SS.seek 0ml @18:44:30.03 Exhaust.select :close @18:44:30.14 SSV.dial CBV @18:44:30.18 CBV.dial SSV @18:44:30.94 Allowing 130s for 25.0ml of remaining 425.0ml @18:44:30.95 Intake.select :open @18:44:33.12 Intake @15.2psia, vacuum from 9.7 to 12.7psi @18:44:33.14 SS.reconfigure :inhaling 0.38ml/s @18:44:33.19 SS.seek 25ml @18:45:09.34 recoverPSRreagent08 @18:45:09.35 PTV.dial :puck @18:45:09.81 PBV.dial :waste1 @18:45:10.23 delay 30 seconds @18:45:40.25 PBV.dial between PSV and waste1 @18:45:40.62 PSR.select :air @18:45:40.63 PSV.dial PSR @18:45:41.40 PS.reconfigure :fast @18:45:41.45 PS.seek 2.5ml @18:45:42.43 Stroke completed -- waiting for pressure to equalize ... @18:45:42.45 SS.reconfigure :exhale @18:45:42.50 SS.hold @18:46:05.63 PSV.dial PTV @18:46:06.40 delay 5 seconds @18:46:11.41 PSV.dial PSR @18:46:12.16 delay 5 seconds @18:46:17.18 PSV.dial PTV @18:46:18.05 delay 5 seconds @18:46:22.68 SS.reconfigure :default @18:46:22.73 SS.hold @18:46:22.80 Intake.select :close @18:46:22.95 ShallowSampler::Clogged in WCR -- Intake @15psia & Outlet @12psia won't equalize after 600.0ml @18:46:22.97 SSV.dial :exhaust @18:46:23.12 PSV.dial :air @18:46:23.75 Exhaust.select :open @18:46:23.86 SS.reconfigure :exhale @18:46:23.94 PS.seek 0ml @18:46:23.98 SS.seek 0ml @18:46:32.81 Exhaust.select :close @18:46:32.92 Sampled 600.0ml @18:46:32.94 Drying puck before fixing for 5:00 @18:46:32.95 CBV.dial :waste2b @18:46:33.71 delay 1:00 @18:46:47.63 washCycleDA #2 of 3 @18:46:47.64 PSR.select :air @18:46:47.66 PSV.dial PSR @18:46:48.99 Pulling 0.5ml of PSR.wash @18:46:49.00 PSR.select :wash @18:46:49.12 PS.reconfigure :default @18:46:49.17 PS.seek 0.5ml @18:46:55.07 delay 5 seconds @18:47:00.09 PSR.select :air @18:47:00.21 PS.seek 2ml @18:47:16.56 PTV.dial :puck @18:47:16.58 PSV.dial PTV @18:47:17.32 PBV.dial :waste1 @18:47:17.74 PS.seek 1.5ml @18:47:23.69 delay 3 seconds @18:47:26.71 PBV.dial between PSV and waste1 @18:47:27.07 PS.seek 0ml @18:47:33.73 CSV.dial :air @18:47:34.67 CS.reconfigure :fast @18:47:34.72 CS.seek 10ml @18:47:43.22 delay 2:00 @18:47:59.48 Pumping wcr puck from tube 3 up to 35psi... @18:47:59.50 CBV.dial SSV @18:48:00.78 CBV.dial between CSV and waste2b @18:48:01.74 CSV.dial between air and CTV @18:48:02.06 CS.seek 7.134ml @18:48:09.39 CTV.dial CSV,avoiding: :air @18:48:09.42 CSV.dial CTV @18:48:10.26 CS.reconfigure :pressurize @18:48:10.32 CS.seek 0ml @18:48:29.07 CSV.dial :air @18:48:29.53 CS.reconfigure :fast @18:48:29.58 CS.seek 10ml @18:48:53.97 CSV.dial between air and CTV @18:48:54.30 CS.seek 4.133ml @18:49:08.51 CTV.dial CSV,avoiding: :air @18:49:08.54 CSV.dial CTV @18:49:09.01 CS.reconfigure :pressurize @18:49:09.06 CS.seek 0ml @18:49:20.13 CSV.dial :air @18:49:20.57 CS.reconfigure :fast @18:49:20.63 CS.seek 10ml @18:49:43.25 recoverPSRreagent08 @18:49:43.26 PTV.dial :puck @18:49:43.28 PBV.dial :waste1 @18:49:43.63 delay 30 seconds @18:49:45.28 CSV.dial between air and CTV @18:49:45.61 CS.seek 4.213ml @18:49:59.67 CTV.dial CSV,avoiding: :air @18:49:59.70 CSV.dial CTV @18:50:00.06 CS.reconfigure :pressurize @18:50:00.11 CS.seek 0ml @18:50:11.85 CSV.dial :air @18:50:12.31 CS.reconfigure :fast @18:50:12.36 CS.seek 10ml @18:50:13.64 PBV.dial between PSV and waste1 @18:50:14.00 PSR.select :air @18:50:14.02 PSV.dial PSR @18:50:14.79 PS.reconfigure :fast @18:50:14.84 PS.seek 2.5ml @18:50:36.94 CSV.dial between air and CTV @18:50:37.29 CS.seek 4.236ml @18:50:38.81 PSV.dial PTV @18:50:39.58 delay 5 seconds @18:50:44.60 PSV.dial PSR @18:50:45.35 delay 5 seconds @18:50:50.37 PSV.dial PTV @18:50:51.13 delay 5 seconds @18:50:51.23 CTV.dial CSV,avoiding: :air @18:50:51.26 CSV.dial CTV @18:50:51.73 CS.reconfigure :pressurize @18:50:51.78 CS.seek 0ml @18:50:56.14 PSV.dial :air @18:50:57.02 PS.seek 0ml @18:51:03.16 CSV.dial :air @18:51:03.60 CS.reconfigure :default @18:51:03.65 CS.hold @18:51:03.79 ShallowSampler::Error in MAIN -- Puck.from 3, :wcr reached only 20.0 of 35psi after pumping in 40ml of gas @18:51:03.80 Waiting to Resume from Checkpoint "Pumping wcr puck from tube 3 up to 35psi..." @18:51:03.82 BadNews.email "Puck.from 3, :wcr reached only 20.0 of 35psi after pumping in 40ml of gas",Subject: "ShallowSampler::Error in MAIN" @18:51:08.02 upsync @18:51:20.57 washCycleDA #3 of 3 @18:51:20.58 PSR.select :air @18:51:20.60 PSV.dial PSR @18:51:21.92 Pulling 0.5ml of PSR.wash @18:51:21.93 PSR.select :wash @18:51:22.06 PS.reconfigure :default @18:51:22.11 PS.seek 0.5ml @18:51:28.06 delay 5 seconds @18:51:33.08 PSR.select :air @18:51:33.21 PS.seek 2ml @18:51:49.37 PTV.dial :puck @18:51:49.39 PSV.dial PTV @18:51:50.16 PBV.dial :waste1 @18:51:51.09 PS.seek 1.5ml @18:51:57.09 delay 3 seconds @18:52:00.11 PBV.dial between PSV and waste1 @18:52:00.47 PS.seek 0ml @18:52:16.78 delay 2:00 @18:54:16.81 recoverPSRreagent08 @18:54:16.82 PTV.dial :puck @18:54:16.83 PBV.dial :waste1 @18:54:17.20 delay 30 seconds @18:54:47.23 PBV.dial between PSV and waste1 @18:54:47.60 PSR.select :air @18:54:47.62 PSV.dial PSR @18:54:48.39 PS.reconfigure :fast @18:54:48.44 PS.seek 2.5ml @18:55:12.52 PSV.dial PTV @18:55:13.29 delay 5 seconds @18:55:18.31 PSV.dial PSR @18:55:19.06 delay 5 seconds @18:55:24.08 PSV.dial PTV @18:55:24.95 delay 5 seconds @18:55:29.97 PSV.dial :air @18:55:30.75 PS.seek 0ml @18:55:54.21 Gate.power :camera,:ON @18:55:54.23 Mixing DA Substrates @18:55:54.24 PSR.select :air @18:55:54.25 PSV.dial PSR @18:55:55.66 Pulling 0.25ml of PSR.sub1 @18:55:55.68 PSR.select :sub1 @18:55:55.89 PS.reconfigure :default @18:55:56.00 PS.seek 0.25ml @18:55:59.41 delay 5 seconds @18:56:04.43 Pulling 0.25ml of PSR.sub2 @18:56:04.45 PSR.select :sub2 @18:56:04.56 PS.seek 0.5ml @18:56:07.90 delay 5 seconds @18:56:12.92 PSR.select :air @18:56:13.03 PS.seek 2ml @18:56:29.24 PTV.dial :puck @18:56:29.26 PSV.dial PTV @18:56:30.02 PBV.dial :waste1 @18:56:30.46 PS.seek 1.5ml @18:56:36.43 delay 3 seconds @18:56:39.45 PBV.dial between PSV and waste1 @18:56:39.83 PS.seek 0ml @18:56:55.96 delay 10 seconds @18:57:05.99 recoverPSRreagent08 @18:57:06.00 PTV.dial :puck @18:57:06.01 PBV.dial :waste1 @18:57:06.38 delay 30 seconds @18:57:36.41 PBV.dial between PSV and waste1 @18:57:36.79 PSR.select :air @18:57:36.81 PSV.dial PSR @18:57:37.59 PS.reconfigure :fast @18:57:37.64 PS.seek 2.5ml @18:58:01.99 PSV.dial PTV @18:58:02.77 delay 5 seconds @18:58:07.79 PSV.dial PSR @18:58:08.55 delay 5 seconds @18:58:13.58 PSV.dial PTV @18:58:14.35 delay 5 seconds @18:58:19.37 PSV.dial :air @18:58:20.26 PS.seek 0ml @18:58:44.15 Additional air clear @18:58:44.16 PSR.select :air @18:58:44.18 PSV.dial PSR @18:58:45.54 Pulling 2ml of PSR.air @18:58:45.55 PSR.select :air @18:58:45.57 PS.reconfigure :default @18:58:45.62 PS.seek 2ml @18:59:07.37 delay 5 seconds @18:59:12.39 PTV.dial :puck @18:59:12.41 PSV.dial PTV @18:59:13.18 PBV.dial :waste1 @18:59:13.62 PS.seek 1.5ml @18:59:19.49 delay 3 seconds @18:59:22.51 PBV.dial between PSV and waste1 @18:59:22.88 delay 1 second @18:59:23.90 PSV.dial :waste1 @18:59:24.96 PS.seek 0ml @18:59:41.10 Processing Puck Heater at 28.1C before imaging @18:59:41.11 PH.coast @18:59:41.23 Imaging da puck in Processing Clamp while storing it to tube 2 @18:59:41.41 SC.reconfigure :tight @18:59:41.46 SC.seek 2 @18:59:45.08 SC.configure :limp @18:59:45.29 Elbow.reconfigure :tight @18:59:45.34 Elbow.seek 2 @18:59:47.13 Forearm.reconfigure :tight @18:59:47.18 Forearm.seek 2 + 450 counts @18:59:48.21 SE.reconfigure :pick @18:59:48.27 SE.seek :beamBreak @18:59:51.35 SE.configure :default @18:59:51.50 Elbow.reconfigure :limp @18:59:51.56 Forearm.reconfigure :loose @18:59:51.61 Forearm.seek 2 @18:59:51.88 Elbow.hold @18:59:51.95 Elbow.reconfigure :tight @18:59:52.05 SE.seek 0.16 Pucks below Top Plate @18:59:52.51 Forearm.force -20 @18:59:52.56 Forearm.seek 2 + 303 counts @18:59:52.87 Forearm.reconfigure :tight @18:59:52.92 Forearm.seek 2 + 450 counts @18:59:53.13 SE.seek 1.16 Pucks below Top Plate @18:59:53.65 Forearm.reconfigure :loose @18:59:53.70 Forearm.seek :retract @18:59:55.12 Elbow.seek PC @18:59:57.61 Elbow.reconfigure :loose @18:59:57.71 Forearm.seek PC @18:59:58.64 Hand.close @18:59:59.23 PC.seek :open @19:00:04.00 Forearm.seek :retract @19:00:04.97 Elbow.reconfigure :tight @19:00:05.02 Elbow.seek 2 @19:00:07.63 Forearm.reconfigure :tight @19:00:07.68 Forearm.seek 2 + 450 counts @19:00:08.67 Hand.open @19:00:09.41 SE.seek 0.81 Pucks below Top Plate @19:00:09.67 SE.reconfigure :seal @19:00:09.72 SE.seek -0.15 Pucks below Top Plate @19:00:10.30 Lights, camera, ACTION! @19:00:10.33 PuckCamera: snap -bin=2,2 -auto /var/log/esp/da23oct1619h750ml.tif @19:00:29.78 Waiting >20 seconds for da to relinquish FlushPuck @19:00:34.35 SX HX9/16 (USB:09): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 20.68 seconds /var/log/esp/da23oct1619h750ml.tif: TIFF Upload Complete @19:00:34.38 PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da23oct1619h750ml15s.tif @19:00:50.03 SX HX9/16 (USB:09): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15 seconds /var/log/esp/midres/da23oct1619h750ml15s.tif: TIFF Upload Complete @19:00:50.06 PuckCamera: snap -bin=2,2 20.0 /var/log/esp/midres/da23oct1619h750ml20s.tif @19:01:10.68 SX HX9/16 (USB:09): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 20 seconds /var/log/esp/midres/da23oct1619h750ml20s.tif: TIFF Upload Complete @19:01:10.69 Gate.power :camera,:OFF @19:01:10.71 DAfans.email "20.68 sec auto-exposure",Subject: "da23oct1619h750ml images!" @19:01:10.98 Elbow.seek 2 @19:01:11.40 Forearm.seek 2 + 450 counts @19:01:11.60 SE.reconfigure :gently @19:01:11.68 SE.seek 0.56 Pucks below Top Plate @19:01:12.33 Elbow.reconfigure :limp @19:01:12.38 Forearm.reconfigure :loose @19:01:12.45 Forearm.seek 2 @19:01:12.77 Elbow.hold @19:01:12.84 Elbow.reconfigure :tight @19:01:12.92 SE.reconfigure :default @19:01:12.98 SE.seek 0.75 Pucks below Top Plate @19:01:14.01 Forearm.force -20 @19:01:14.13 Forearm.seek 2 + 292 counts @19:01:14.66 Forearm.reconfigure :tight @19:01:14.71 Forearm.seek 2 + 450 counts @19:01:15.15 SE.seek :down @19:01:16.43 Forearm.reconfigure :loose @19:01:16.53 Forearm.seek :retract @19:01:17.89 SE.stop @19:01:18.10 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @19:01:18.49 Elbow.seek FlushPuck::Garage @19:01:20.56 Elbow.reconfigure :loose @19:01:20.76 Forearm.seek :garage @19:01:21.77 Hand.close @19:01:22.72 Forearm.seek :retract @19:01:23.71 Elbow.reconfigure :tight @19:01:23.83 Elbow.seek PC @19:01:25.27 Elbow.reconfigure :loose @19:01:25.49 Forearm.reconfigure :tight @19:01:25.59 Forearm.seek PC @19:01:27.30 PC.seek :closed @19:01:32.08 Processing Clamp onSpring/offSpring for 16/26 samples, on/off torque 1.61 @19:01:32.22 Hand.open @19:01:33.00 Forearm.reconfigure :loose @19:01:33.09 Forearm.seek :retract @19:01:35.05 PSR.select :air @19:01:35.09 PSV.dial PSR @19:01:35.63 Pulling 0.5ml of PSR.flush @19:01:35.67 PSR.select :flush @19:01:35.85 PS.seek 0.5ml @19:01:41.82 delay 5 seconds @19:01:45.16 upsync @19:01:46.85 PSR.select :air @19:01:46.97 PS.seek 2.5ml @19:02:08.27 delay 1 second @19:02:09.29 PRV.dial :waste2,avoiding: :ab2 @19:02:11.14 PTV.dial :PRVmixing @19:02:12.46 PSV.dial :mixloop @19:02:13.50 PS.seek :empty @19:02:39.83 PSR.select :air @19:02:39.85 PSV.dial PSR @19:02:40.89 Pulling 0.5ml of PSR.flush @19:02:40.90 PSR.select :flush @19:02:41.02 PS.seek 0.5ml @19:02:47.02 delay 5 seconds @19:02:52.04 PSR.select :air @19:02:52.16 PS.seek 2.5ml @19:03:13.61 delay 1 second @19:03:14.63 PBV.dial :waste1 @19:03:15.00 PTV.dial :mixing @19:03:15.75 PSV.dial :mixloop @19:03:16.93 PS.seek :empty @19:03:43.30 PSV.dial PSR @19:03:44.33 PSR.select :flush @19:03:44.45 PS.seek 1ml @19:03:55.51 PSR.select :air @19:03:55.63 PS.seek 2.5ml @19:04:11.83 PSV.dial PTV @19:04:12.58 PTV.dial :puck @19:04:13.31 PBV.dial :waste1 @19:04:13.40 PS.seek 0ml @19:04:39.70 PSV.dial PSR @19:04:40.45 PSR.select :flush @19:04:40.57 PS.seek 0.5ml @19:04:46.58 PSR.select :air @19:04:46.70 PS.seek 2.5ml @19:05:07.98 PSV.dial PTV @19:05:08.73 PBV.dial PSV @19:05:09.30 PS.seek 0ml @19:05:35.57 PSV.dial PBV @19:05:36.05 PS.seek 2.5ml @19:06:02.42 PSV.dial :waste1 @19:06:03.16 PS.seek 0ml @19:06:29.51 PSV.dial PSR @19:06:29.98 PSR.select :air @19:06:30.01 PS.seek 2.5ml @19:06:56.79 PSV.dial PTV @19:06:57.53 PBV.dial PSV @19:06:57.72 PS.seek 0ml @19:07:24.26 PSV.dial PBV @19:07:24.74 PS.seek 2.5ml @19:07:51.07 PSV.dial :waste1 @19:07:51.81 PS.seek 0ml @19:08:18.20 PSV.dial PSR @19:08:18.67 PSR.select :flush @19:08:18.79 PS.seek 1ml @19:08:29.79 PSR.select :air @19:08:29.91 PS.seek 2.5ml @19:08:46.01 PSV.dial PTV @19:08:46.74 PTV.dial :puck @19:08:46.76 PBV.dial :waste1 @19:08:47.30 PS.seek 0ml @19:09:13.66 PSV.dial PSR @19:09:14.41 PSR.select :flush @19:09:14.54 PS.seek 0.5ml @19:09:20.51 PSR.select :air @19:09:20.63 PS.seek 2.5ml @19:09:41.85 PSV.dial PTV @19:09:42.60 PBV.dial PSV @19:09:43.17 PS.seek 0ml @19:10:09.69 PSV.dial PBV @19:10:10.17 PS.seek 2.5ml @19:10:36.47 PSV.dial :waste1 @19:10:37.22 PS.seek 0ml @19:11:03.54 PSV.dial PSR @19:11:04.01 PSR.select :air @19:11:04.03 PS.seek 2.5ml @19:11:30.44 PSV.dial PTV @19:11:31.18 PBV.dial PSV @19:11:31.26 PS.seek 0ml @19:11:57.69 PSV.dial PBV @19:11:58.17 PS.seek 2.5ml @19:12:24.57 PSV.dial :waste1 @19:12:25.31 PS.seek 0ml @19:12:51.66 Garaging FlushPuck in Processing Clamp @19:12:51.73 Elbow.reconfigure :tight @19:12:51.78 Elbow.seek PC @19:12:51.90 Elbow.reconfigure :loose @19:12:52.45 Forearm.seek PC @19:12:53.40 Hand.close @19:12:53.97 PC.seek :open @19:12:58.69 Forearm.seek :retract @19:12:59.65 Elbow.reconfigure :tight @19:12:59.70 Elbow.seek :garage @19:13:01.03 Elbow.reconfigure :loose @19:13:01.13 Forearm.reconfigure :tight @19:13:01.18 Forearm.seek :garage @19:13:02.26 Hand.open @19:13:02.90 Forearm.reconfigure :loose @19:13:02.94 Forearm.seek :retract @19:20:11.10 Session Begun @19:20:20.09 -> CC Collection Clamp contains wcr puck from tube 3 @19:20:25.50 -> PC Processing Clamp open @19:21:02.34 -> Puck.count @19:21:02.42 SE.seek :down @19:21:02.51 SE.stop @19:21:02.62 SC.reconfigure :tight @19:21:02.69 SC.seek 1 @19:21:06.31 SC.configure :limp @19:21:06.55 Elbow.reconfigure :tight @19:21:06.62 Elbow.seek 1 @19:21:08.48 Forearm.reconfigure :tight @19:21:08.55 Forearm.seek 1 + 450 counts @19:21:09.78 SE.reconfigure :place @19:21:09.86 SE.seek :beamBreak @19:21:10.95 SE.configure :default @19:21:11.08 SE.seek :down @19:21:11.53 SE.stop @19:21:12.21 Forearm.reconfigure :loose @19:21:12.29 Forearm.seek :retract @19:21:13.27 SC.reconfigure :tight @19:21:13.35 SC.seek 2 @19:21:16.93 SC.configure :limp @19:21:17.11 Elbow.seek 2 @19:21:17.54 Forearm.reconfigure :tight @19:21:18.00 Forearm.seek 2 + 450 counts @19:21:19.20 SE.reconfigure :place @19:21:19.29 SE.seek :beamBreak @19:21:22.58 SE.configure :default @19:21:22.71 SE.seek :down @19:21:23.85 Forearm.reconfigure :loose @19:21:23.92 Forearm.seek :retract @19:21:25.29 SE.stop @19:21:25.36 SC.reconfigure :tight @19:21:25.42 SC.seek 3 @19:21:28.89 SC.configure :limp @19:21:29.06 Elbow.seek 3 @19:21:29.41 Forearm.reconfigure :tight @19:21:29.48 Forearm.seek 3 + 450 counts @19:21:30.50 SE.reconfigure :place @19:21:30.59 SE.seek :beamBreak @19:21:31.97 SE.configure :default @19:21:32.10 SE.seek :down @19:21:32.78 SE.stop @19:21:33.23 Forearm.reconfigure :loose @19:21:33.30 Forearm.seek :retract @19:21:34.33 SC.reconfigure :tight @19:21:34.42 SC.seek 4 @19:21:38.07 SC.configure :limp @19:21:38.25 Elbow.seek 4 @19:21:38.94 Forearm.reconfigure :tight @19:21:39.01 Forearm.seek 4 + 450 counts @19:21:40.14 SE.reconfigure :place @19:21:40.22 SE.seek :beamBreak @19:21:41.37 SE.configure :default @19:21:41.50 SE.seek :down @19:21:41.93 SE.stop @19:21:42.63 Forearm.reconfigure :loose @19:21:42.71 Forearm.seek :retract @19:21:43.63 SC.reconfigure :tight @19:21:43.70 SC.seek 5 @19:21:47.23 SC.configure :limp @19:21:47.86 Elbow.seek 5 @19:21:48.08 Forearm.reconfigure :tight @19:21:48.15 Forearm.seek 5 + 450 counts @19:21:49.25 SE.reconfigure :place @19:21:49.33 SE.seek :beamBreak @19:21:50.50 SE.configure :default @19:21:50.63 SE.seek :down @19:21:51.10 SE.stop @19:21:51.76 Forearm.reconfigure :loose @19:21:51.84 Forearm.seek :retract @19:21:52.86 SC.reconfigure :tight @19:21:52.94 SC.seek 6 @19:21:56.70 SC.configure :limp @19:21:56.88 Elbow.seek 6 @19:21:57.10 Forearm.reconfigure :tight @19:21:57.17 Forearm.seek 6 + 450 counts @19:21:58.26 SE.reconfigure :place @19:21:58.39 SE.seek :beamBreak @19:21:59.60 SE.configure :default @19:21:59.72 SE.seek :down @19:22:00.13 SE.stop @19:22:00.86 Forearm.reconfigure :loose @19:22:00.93 Forearm.seek :retract @19:22:01.95 SC.reconfigure :tight @19:22:02.02 SC.seek 7 @19:22:05.62 SC.configure :limp @19:22:05.80 Elbow.seek 7 @19:22:06.42 Forearm.reconfigure :tight @19:22:06.49 Forearm.seek 7 + 450 counts @19:22:07.55 SE.reconfigure :place @19:22:07.63 SE.seek :beamBreak @19:22:08.94 SE.configure :default @19:22:09.06 SE.seek :down @19:22:09.74 SE.stop @19:22:10.19 Forearm.reconfigure :loose @19:22:10.62 Forearm.seek :retract {1 => 33, 2 => 2, 3 => 30, 4 => 33, 5 => 33, 6 => 33, 7 => 31} @19:23:40.91 -> CC.unloadPuck 2, :WCR @19:23:41.00 Puck::Warning in dave -- Unloading Puck.from 3, :wcr to Storage Carousel @19:23:41.02 Storing WCR puck in Collection Clamp to tube 2 @19:23:41.22 SC.reconfigure :tight @19:23:41.29 SC.seek 2 @19:23:57.51 SC.configure :limp @19:23:57.68 Elbow.seek 2 @19:23:58.22 Forearm.reconfigure :tight @19:23:58.30 Forearm.seek 2 + 450 counts @19:23:59.34 SE.reconfigure :pick @19:23:59.47 SE.seek :beamBreak @19:24:02.52 SE.configure :default @19:24:02.70 Elbow.reconfigure :limp @19:24:02.77 Forearm.reconfigure :loose @19:24:02.84 Forearm.seek 2 @19:24:03.14 Elbow.hold @19:24:03.23 Elbow.reconfigure :tight @19:24:03.35 SE.seek 1.17 Pucks below Top Plate @19:24:03.81 Forearm.force -20 @19:24:03.87 Forearm.seek 2 + 286 counts @19:24:04.16 Forearm.reconfigure :tight @19:24:04.24 Forearm.seek 2 + 450 counts @19:24:04.47 SE.seek 2.17 Pucks below Top Plate @19:24:05.00 Forearm.reconfigure :loose @19:24:05.07 Forearm.seek :retract @19:24:06.03 Elbow.seek CC @19:24:07.73 Elbow.reconfigure :loose @19:24:07.85 Forearm.seek CC @19:24:08.86 Hand.close @19:24:09.46 CC.seek :open @19:24:14.23 Forearm.seek :retract @19:24:15.23 Elbow.reconfigure :tight @19:24:15.30 Elbow.seek 2 @19:24:17.13 Elbow.reconfigure :limp @19:24:17.20 Forearm.seek 2 @19:24:18.20 Elbow.hold @19:24:18.29 Elbow.reconfigure :tight @19:24:18.75 Hand.open @19:24:19.57 Forearm.force -20 @19:24:19.63 Forearm.seek 2 + 292 counts @19:24:20.01 Forearm.reconfigure :tight @19:24:20.08 Forearm.seek 2 + 450 counts @19:24:20.38 SE.seek :down @19:24:21.51 Forearm.reconfigure :loose @19:24:21.58 Forearm.seek :retract @19:24:22.94 SE.stop @19:24:28.78 -> CC Collection Clamp open @19:24:39.96 -> Flush FlushPuck is in FlushPuck::Garage @19:24:42.34 -> PC Processing Clamp open @19:24:44.65 -> Arm Elbow at 4, Forearm retracted, Hand is opened @19:25:03.84 -> abort! 'mission complete' @19:25:03.86 CmdServer::Client::Error in dave -- Aborting Mission: mission complete @19:25:03.90 BadNews.email "mission complete",Subject: "DA_WCR_101623 ABORTED!" # @19:25:08.17 upsync @19:25:08.26 Session Ended + ESPwhich DA_WCR_101823.rb + fn=DA_WCR_101823.rb + IFS=: + set /home/esp/esp2/mission . + fullPath=/home/esp/esp2/mission/DA_WCR_101823.rb + [ -f /home/esp/esp2/mission/DA_WCR_101823.rb ] + realpath /home/esp/esp2/mission/DA_WCR_101823.rb + return 0 + scriptPath=/home/esp/esp2local/mission/DA_WCR_101823.rb + exec ruby /home/esp/esp2/lib/esp.rb /home/esp/esp2local/mission/DA_WCR_101823.rb @15:32:07.99UTC18-Oct-23
ESP2 v3.42 I2C Gateway v3.20 for core bus @15:32:10.68 Configuration for short puck jake 1/11/21 by brent@mbari.org /home/esp/esp2/type/shallow/jake/configure.rb @15:32:14.33 Polling Can every 10:00 @15:32:14.40 Cleanly exited at 15:28:50UTC18-Oct-23 @15:32:14.45 Loading /home/esp/esp2local/mission/DA_WCR_101823.rb mission @15:32:14.51 Preparing to sleep until + 00:00:05 @15:32:14.54 GoodNews.email "Starting DA_WCR_101823 w/pucks in tube 3\n{1 => 33,\n 2 => 3,\n 3 => 30,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@15:32:14, 28% flow, 17.3C, 19% humidity, 14.6psia, 13.749V, 0.284A, 177.215Ah, 3.91W",Subject: "DA_WCR_101823 begun" @15:32:14.59
Asleep til + 00:00:05 @15:32:14.61 Continuing at 15:32 @15:32:14.85 Gate.power :core,:OFF @15:32:14.86 Gate.power :raw,:OFF @15:32:14.87 Shutdown @15:32:17.19 GoodNews.email "Then, in tube 3: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_101823.rb:14\n{1 => 33,\n 2 => 3,\n 3 => 30,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@15:32:14, 28% flow, 17.3C, 19% humidity, 14.6psia, 13.749V, 0.284A, 177.215Ah, 3.91W",Subject: "Asleep until 15:32" @15:32:18.57 upsync @15:32:19.49
Powering core bus @15:32:21.85 upsync @15:32:24.84
Gate.power :raw,:ON @15:32:25.62 CS.configure :default @15:32:25.67 PS.configure :default @15:32:25.72 SS.configure :default @15:32:25.77 CC.configure :default @15:32:25.81 PC.configure :default @15:32:25.88 Forearm.configure :loose @15:32:25.93 Elbow.configure :loose @15:32:26.00 SC.configure :loose @15:32:26.05 SE.configure :default @15:32:26.10 CH.configure :default @15:32:26.13 PH.configure :default @15:32:26.17 All dwarf controllers running firmware version 3.85 @15:32:26.28 Valve.reset @15:32:26.55 Intake.select :close @15:32:26.59 Exhaust.select :close @15:32:26.60 CTV.dial :air @15:32:26.67 PSV.dial :air @15:32:26.81
Forearm.home.seek :retract @15:32:27.57 Hand.close @15:32:27.61
Elbow.reconfigure :loose @15:32:27.69 Elbow.jog 1500 @15:32:27.94 PS.home.seek 0ml @15:32:28.00 CSV.dial :waste1 @15:32:28.20
Elbow.reconfigure :loose @15:32:28.25 Elbow.home.seek :clear @15:32:28.72 SSV.dial :spit,avoiding: :exhaust @15:32:29.60 CS.reconfigure :fast @15:32:29.64 SS.home.seek 0ml @15:32:29.72 CS.home.seek 0ml @15:32:32.77
SE.home.seek :down @15:32:33.38 SC.reconfigure :tight @15:32:33.43 SC.home.seek :initial @15:32:36.20 PS.stop @15:33:01.70
SC.configure :limp @15:33:01.75 Forearm.seek :retract @15:33:01.82 Elbow.seek :hammer @15:33:02.82 Forearm.reconfigure :tight @15:33:02.87 Forearm.seek retracted @15:33:03.26 PC.home.seek :open @15:33:03.66
CC.home.seek :open @15:33:07.02 Phase [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] @15:33:07.03 Notice.email "At 15:33:06 began in tube 3: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_101823.rb:14\n{1 => 33,\n 2 => 3,\n 3 => 30,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@15:33:06, 28% flow, 17.3C, 19% humidity, 14.6psia, 13.676V, 0.284A, 177.221Ah, 3.89W",Subject: "Started DA_WCR_101823 phase initialPurge; da [1000, 1000] #da and wsr are run simultanelously" @15:33:07.06
Starting initialPurge @15:33:07.08 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @15:33:07.14 Hand.open @15:33:07.94 Elbow.reconfigure :tight @15:33:07.99 Elbow.seek FlushPuck::Garage @15:33:09.96 Elbow.reconfigure :loose @15:33:10.07 Forearm.reconfigure :loose @15:33:10.13 Forearm.seek :garage @15:33:11.02 upsync @15:33:11.15
Hand.close @15:33:11.93 Forearm.seek :retract @15:33:12.91 Elbow.reconfigure :tight @15:33:12.96 Elbow.seek CC @15:33:15.94 Elbow.reconfigure :loose @15:33:16.05 Forearm.reconfigure :tight @15:33:16.10 Forearm.seek CC @15:33:17.33 CC.seek :closed @15:33:21.89 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.35 @15:33:21.97 Hand.open @15:33:22.62 Forearm.reconfigure :loose @15:33:22.66 Forearm.seek :retract @15:33:23.62 Flushing the Collection Valve::Manifold @15:33:23.74 CSV.dial CSR @15:33:25.05 Pulling 5ml of CSR.flush @15:33:25.06 CSR.select :flush @15:33:25.18 CS.reconfigure :default @15:33:25.64 CS.seek 5ml @15:33:39.25 delay 5 seconds @15:33:44.27 CSR.select :air @15:33:44.44 CS.reconfigure :fast @15:33:44.49 CS.seek 7.501ml @15:33:51.31 CSV.dial :waste @15:33:52.60 CS.reconfigure :default @15:33:52.65 CS.seek 0ml @15:34:12.88 Purging external Intake @15:34:13.00 CSV.dial :flush @15:34:13.44 CS.reconfigure :fast @15:34:13.49 CS.seek 10ml @15:34:38.03 delay 5 seconds @15:34:43.05 CSV.dial CBV @15:34:43.09
CBV.dial CSV @15:34:43.86 CTV.dial :intake @15:34:44.62 Intake.select :open @15:34:44.74 CS.seek 0ml @15:35:08.34 Intake.select :close @15:35:08.44 CTV.dial :air @15:35:09.24 Flushing upper manifold @15:35:09.36 CSV.dial :flush @15:35:10.10 CS.seek 5ml @15:35:22.75 delay 5 seconds @15:35:27.77 CSV.dial :air @15:35:28.28 CS.seek 7ml @15:35:33.78 CSV.dial CBV @15:35:33.81
CBV.dial CSV @15:35:34.83 CTV.dial :air @15:35:34.91 CS.seek 0ml @15:35:51.47 delay 7 seconds @15:35:58.50 CS.seek 3ml @15:36:06.72 CSV.dial :waste @15:36:07.18 CS.seek 0ml @15:36:14.92 CSV.dial :flush @15:36:15.36 CS.seek 3ml @15:36:23.62 delay 5 seconds @15:36:28.64 CSV.dial :air @15:36:29.28 CS.seek 5ml @15:36:34.96 CSV.dial :air @15:36:35.05 CS.seek 0ml @15:36:47.15 delay 7 seconds @15:36:54.18 CS.seek 3ml @15:37:02.18 CSV.dial :waste @15:37:02.91 CS.seek 0ml @15:37:10.56 Garaging FlushPuck in Collection Clamp @15:37:10.63 Elbow.reconfigure :tight @15:37:10.73 Elbow.seek CC @15:37:10.98 Elbow.reconfigure :loose @15:37:11.08 Forearm.seek CC @15:37:12.11 Hand.close @15:37:12.68 CC.seek :open @15:37:17.74 Forearm.seek :retract @15:37:18.69 Elbow.reconfigure :tight @15:37:18.74 Elbow.seek :garage @15:37:21.74 Elbow.reconfigure :loose @15:37:21.84 Forearm.reconfigure :tight @15:37:21.89 Forearm.seek :garage @15:37:22.94 Hand.open @15:37:23.61 Forearm.reconfigure :loose @15:37:23.65 Forearm.seek :retract @15:37:24.69 da sampling at most 1000ml, wcr at most 1000ml @15:37:24.73 Loading sh1 puck from tube 3 into Collection Clamp @15:37:24.75 DAfans.email "Started da in tube 3 at 15:37:24UTC18-Oct-23\n{1 => 33,\n 2 => 3,\n 3 => 30,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@15:33:06, 28% flow, 17.3C, 19% humidity, 14.6psia, 13.676V, 0.284A, 177.221Ah, 3.89W",Subject: "da sampling at most 1000ml, wcr at most 1000ml" @15:37:25.03
SC.reconfigure :tight @15:37:25.08 SC.seek 3 @15:37:28.71 SC.configure :limp @15:37:28.95 Elbow.reconfigure :tight @15:37:28.98 upsync @15:37:29.03
Elbow.seek 3 @15:37:30.99 Forearm.reconfigure :tight @15:37:31.04 Forearm.seek 3 + 450 counts @15:37:32.13 SE.reconfigure :pick @15:37:32.19 SE.seek :beamBreak @15:37:33.31 SE.configure :default @15:37:33.48 Elbow.seek 3 @15:37:33.66 Forearm.seek 3 + 300 counts @15:37:33.91 Forearm.reconfigure :loose @15:37:33.96 Hand.close @15:37:34.56 SE.seek :down @15:37:34.77
Forearm.seek :retract @15:37:35.35 SE.stop @15:37:36.11
Elbow.seek CC @15:37:37.66 Elbow.reconfigure :loose @15:37:37.77 Forearm.reconfigure :tight @15:37:37.82 Forearm.seek CC @15:37:39.05 CC.seek :closed @15:37:43.56 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.20 @15:37:43.57 Hand.open @15:37:44.23 Forearm.reconfigure :loose @15:37:44.28 Forearm.seek :retract @15:37:45.24 collectSample 1000ml w/bubble point of 24psi @15:37:45.25 Priming 7ml @15:37:45.26 Intake.select :close @15:37:45.27 Exhaust.select :close @15:37:45.28 SS.hold @15:37:45.49 Calibrating pressure and temperature sensors @15:37:45.52 CTV.dial :air @15:37:45.59 SSV.dial :spit,avoiding: :exhaust @15:37:45.78 SS.reconfigure :fast @15:37:45.83 SS.seek 5ml @15:37:48.68 SSV.dial CBV @15:37:48.71
CBV.dial SSV @15:37:50.07 delay 3.8 seconds @15:37:54.00 Sensor limits: Intake max 110.5psia; Outlet max 103.5psia @15:37:54.01 SSV.dial :spit @15:37:55.34 SS.seek 0ml @15:37:58.08 SSV.dial CBV @15:37:59.40 CTV.dial :intake @15:38:00.20 SSV.dial CBV @15:38:00.23
CBV.dial SSV @15:38:00.30 Allowing 40s for 7.0ml of remaining 7.0ml @15:38:00.32 Intake.select :open @15:38:02.49 Intake @14.4psia, vacuum from 8.9 to 11.9psi @15:38:02.51 SS.reconfigure :inhaling 2.20ml/s @15:38:02.56 SS.seek 7ml @15:38:09.21 Stroke completed -- waiting for pressure to equalize ... @15:38:09.22 SS.reconfigure :exhale @15:38:09.26 SS.hold @15:38:40.33 SS.reconfigure :default @15:38:40.37 SS.hold @15:38:40.43 Intake.select :close @15:38:40.59 ShallowSampler::Clogged in MAIN -- Filtering too Slowly after 7.0ml @15:38:40.60 SSV.dial :exhaust @15:38:41.35 Exhaust.select :open @15:38:41.46 SS.reconfigure :exhale @15:38:41.51 SS.seek 0ml @15:38:45.57 Exhaust.select :close @15:38:45.68 Sampling 1000.0ml @15:38:45.69 Intake.select :close @15:38:45.70 Exhaust.select :close @15:38:45.71 SS.reconfigure :default @15:38:45.76 SS.hold @15:38:45.97 CTV.dial :intake @15:38:46.14 SSV.dial CBV @15:38:46.17
CBV.dial SSV @15:38:46.93 Allowing 130s for 25.0ml of remaining 1000.0ml @15:38:46.94 Intake.select :open @15:38:49.10 Intake @14.5psia, vacuum from 9.0 to 12.0psi @15:38:49.12 SS.reconfigure :inhaling 2.20ml/s @15:38:49.17 SS.seek 25ml @15:38:54.09 Vacuum too high -- waiting up to 40 seconds @15:38:54.10 SS.reconfigure :exhale @15:38:54.16 SS.hold @15:39:35.24 SS.reconfigure :default @15:39:35.29 SS.hold @15:39:35.36 Intake.select :close @15:39:35.51 ShallowSampler::Clogged in MAIN -- Intake @14.3psia, & Outlet @3.4psia -- no progress after 4.6ml @15:39:35.53 SSV.dial :exhaust @15:39:36.29 Exhaust.select :open @15:39:36.40 SS.reconfigure :exhale @15:39:36.45 SS.seek 0ml @15:39:39.80 Exhaust.select :close @15:39:39.91 Sampled 4.6ml @15:39:39.92 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @15:39:39.93 Intake.select :close @15:39:40.00 CSV.dial :waste @15:39:40.16 SSV.dial CBV,avoiding: :exhaust @15:39:40.19 CS.seek 0ml @15:39:40.22
CBV.dial SSV @15:39:40.31 CSV.dial :air @15:39:41.05 CS.seek 10ml @15:39:42.10
CTV.dial :air @15:39:42.89 delay 15 seconds @15:39:57.90 SSV.dial :spit,avoiding: :exhaust @15:39:59.21 CTV.dial CSV,avoiding: :intake @15:39:59.25
SS.seek 0ml @15:39:59.38 SSV.dial CBV,avoiding: :exhaust @15:40:05.75 SSV.dial between CBV and plug @15:40:06.23 CBV.dial between SSV and waste2 @15:40:06.64 CTV.dial CSV,avoiding: :air @15:40:06.66
CSV.dial CTV @15:40:07.19 CS.reconfigure :pressurize @15:40:07.24 CS.seek 0ml @15:40:29.25 BubblePoint: Intake @24.2psia, Outlet @-7.9psia @15:40:29.26 CBV.dial SSV @15:40:29.61 SS.reconfigure :default @15:40:29.66 SS.seek 10ml @15:40:35.27 delay 15 seconds @15:40:50.40 Evacuated: Intake @22.2psia, Outlet @16.4psia @15:40:50.41 SSV.dial :spit,avoiding: :exhaust @15:40:51.45 SS.reconfigure :exhale @15:40:51.50 SS.seek 0ml @15:40:56.51 SS.reconfigure :fast @15:40:56.55 SS.seek 5ml @15:40:59.52 SSV.dial CBV,avoiding: :exhaust @15:40:59.55
CBV.dial SSV @15:41:00.88 CTV.dial :air,avoiding: :intake @15:41:02.21 CSV.dial CTV @15:41:02.26
SS.seek 10ml @15:41:03.42 CSV.dial :air @15:41:03.98 CS.reconfigure :fast @15:41:04.03 CS.seek 0ml @15:41:04.91
SSV.dial :spit,avoiding: :exhaust @15:41:06.24 SS.reconfigure :exhale @15:41:06.29 SS.seek 0ml @15:41:08.55 CS.stop @15:41:11.42
SS.reconfigure :fast @15:41:11.91 SS.seek 5ml @15:41:14.66 SSV.dial CBV,avoiding: :exhaust @15:41:14.69
CBV.dial SSV @15:41:17.06 SSV.dial :spit,avoiding: :exhaust @15:41:18.37 SS.reconfigure :default @15:41:18.42 SS.seek 0ml @15:41:21.52 SS.stop @15:41:21.56 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @15:41:21.57 Intake.select :close @15:41:21.64 CSV.dial :waste @15:41:21.76 SSV.dial CBV,avoiding: :exhaust @15:41:21.79
CBV.dial SSV @15:41:22.44 CS.seek 0ml @15:41:22.50 CSV.dial :air @15:41:23.15
CTV.dial :air @15:41:23.23 CS.seek 10ml @15:41:23.37
delay 15 seconds @15:41:38.39 SSV.dial :spit,avoiding: :exhaust @15:41:39.71 CTV.dial CSV,avoiding: :intake @15:41:39.75
SS.reconfigure :exhale @15:41:39.85 SS.seek 0ml @15:41:40.30 SSV.dial CBV,avoiding: :exhaust @15:41:47.84 SSV.dial between CBV and plug @15:41:48.33 CBV.dial between SSV and waste2 @15:41:48.74 CSV.dial between air and CTV @15:41:49.05 CS.seek 8.233ml @15:41:53.97 CTV.dial CSV,avoiding: :air @15:41:53.99
CSV.dial CTV @15:41:54.36 CS.reconfigure :pressurize @15:41:54.41 CS.seek 0ml @15:42:13.16 BubblePoint: Intake @24.2psia, Outlet @-0.3psia @15:42:13.17 CBV.dial SSV @15:42:13.50 SS.reconfigure :default @15:42:13.55 SS.seek 10ml @15:42:19.19 delay 15 seconds @15:42:34.32 Evacuated: Intake @22.6psia, Outlet @16.1psia @15:42:34.33 SSV.dial :spit,avoiding: :exhaust @15:42:35.38 SS.reconfigure :exhale @15:42:35.43 SS.seek 0ml @15:42:40.46 SS.reconfigure :fast @15:42:40.50 SS.seek 5ml @15:42:43.27 SSV.dial CBV,avoiding: :exhaust @15:42:43.30
CBV.dial SSV @15:42:44.63 CTV.dial :air,avoiding: :intake @15:42:45.99 CSV.dial CTV @15:42:46.03
SS.seek 10ml @15:42:47.25 CSV.dial :air @15:42:47.68 CS.reconfigure :fast @15:42:47.73 CS.seek 0ml @15:42:48.72
SSV.dial :spit,avoiding: :exhaust @15:42:50.50 SS.reconfigure :exhale @15:42:50.55 SS.seek 0ml @15:42:51.11 CS.stop @15:42:55.78
SS.reconfigure :fast @15:42:55.83 SS.seek 5ml @15:42:58.65 SSV.dial CBV,avoiding: :exhaust @15:42:58.67
CBV.dial SSV @15:43:01.00 SSV.dial :spit,avoiding: :exhaust @15:43:02.32 SS.reconfigure :default @15:43:02.37 SS.seek 0ml @15:43:05.62 SS.stop @15:43:05.66 Lysing: Adding 2.0ml AqMeOH @15:43:05.67 CBV.dial between CSV and waste1,avoiding: :DBV @15:43:07.16 CTV.dial CSV @15:43:08.49 CSV.dial CSR @15:43:09.28 Pulling 1ml of CSR.AqMeOH @15:43:09.29 CSR.select :AqMeOH @15:43:09.41 CS.reconfigure :default @15:43:09.45 CS.seek 1ml @15:43:12.93 delay 5 seconds @15:43:17.95 CSR.select :air @15:43:18.12 CS.seek 6ml @15:43:31.71 delay 2 seconds @15:43:33.73 CSV.dial :waste2 @15:43:34.17 CS.reconfigure :fast @15:43:34.22 CS.seek 0ml @15:43:48.77 CSV.dial CSR @15:43:49.23 Pulling 2ml of CSR.AqMeOH CSR.select :AqMeOH @15:43:49.35 CS.reconfigure :default @15:43:49.40 CS.seek 2ml @15:43:55.52 delay 5 seconds @15:44:00.54 CSR.select :air @15:44:00.71 CS.seek 4.999ml @15:44:09.40 delay 2 seconds @15:44:11.41 CSV.dial CTV @15:44:11.85 CS.seek 1ml @15:44:22.94 Before lysing Collection Puck Heater at 17.2C/23.9psia @15:44:22.97 Heating Puck.from 3, :sh1 in Collection Clamp to 70C for 10:00 @15:44:22.98 CH.seek 70C,8:00 @15:46:31.51 CH.hold 10:00,70C @15:46:31.70 Begun lysing Collection Puck Heater at 68C/29.5psia @15:56:31.72 Ended lysing Collection Puck Heater at 70C/28.3psia @15:56:31.73 CS.seek 0ml @15:56:35.11 CTV.dial between spare and air,avoiding: :intake @15:56:36.15 CSV.dial :air @15:56:36.73 CS.seek 5ml @15:56:50.41 CSV.dial CTV @15:56:50.85 delay 2 seconds @15:56:52.87 CSV.dial :air @15:56:53.32 delay 2 seconds @15:56:55.34 CSV.dial CTV @15:56:56.26 delay 2 seconds @15:56:58.28 CSV.dial :air @15:56:58.73 delay 2 seconds @15:57:00.75 CS.seek 0ml @15:57:14.44 Puck @56.8psia after final lysis pressurization @15:57:14.45 Recovering Lysate into Collection syringe @15:57:14.46 CSV.dial CBV @15:57:15.48 CBV.dial CSV @15:57:15.87 CS.seek 5ml @15:57:29.66 delay 5 seconds @15:57:34.68 CTV.dial :air @15:57:35.21 CS.seek 6.999ml @15:57:41.10 delay 5 seconds @15:57:46.12 CSV.dial :air2 @15:57:46.56 CS.seek 10ml @15:57:55.10 CSV.dial between air and CTV,avoiding: CBV @15:57:56.26 Lysing Complete @15:57:56.33 Storing sh1 puck in Collection Clamp to tube 2 @15:57:56.51 SC.reconfigure :tight @15:57:56.56 SC.seek 2 @15:58:00.17 SC.configure :limp @15:58:00.39 Elbow.reconfigure :tight @15:58:00.44 Elbow.seek 2 @15:58:02.24 Forearm.reconfigure :tight @15:58:02.29 Forearm.seek 2 + 450 counts @15:58:03.30 SE.reconfigure :pick @15:58:03.36 SE.seek :beamBreak @15:58:06.34 SE.configure :default @15:58:06.50 Elbow.reconfigure :limp @15:58:06.55 Forearm.reconfigure :loose @15:58:06.60 Forearm.seek 2 @15:58:06.84 Elbow.hold @15:58:06.91 Elbow.reconfigure :tight @15:58:07.01 SE.seek 2.17 Pucks below Top Plate @15:58:07.48 Forearm.force -20 @15:58:07.52 Forearm.seek 2 + 285 counts @15:58:07.81 Forearm.reconfigure :tight @15:58:07.85 Forearm.seek 2 + 450 counts @15:58:08.05 SE.seek 3.17 Pucks below Top Plate @15:58:09.01 Forearm.reconfigure :loose @15:58:09.07 Forearm.seek :retract @15:58:10.06 Elbow.seek CC @15:58:11.73 Elbow.reconfigure :loose @15:58:11.84 Forearm.seek CC @15:58:12.78 Hand.close @15:58:13.41 CC.seek :open @15:58:18.15 Forearm.seek :retract @15:58:19.16 Elbow.reconfigure :tight @15:58:19.21 Elbow.seek 2 @15:58:20.97 Elbow.reconfigure :limp @15:58:21.02 Forearm.seek 2 @15:58:22.00 Elbow.hold @15:58:22.06 Elbow.reconfigure :tight @15:58:22.11 Hand.open @15:58:22.90 Forearm.force -20 @15:58:22.95 Forearm.seek 2 + 298 counts @15:58:23.37 Forearm.reconfigure :tight @15:58:23.42 Forearm.seek 2 + 450 counts @15:58:23.69 SE.seek :down @15:58:24.83
Forearm.reconfigure :loose @15:58:24.87 Forearm.seek :retract @15:58:26.18 SE.stop @15:58:26.22
Tranferring DA @15:58:26.23 Beginning samplePassC @15:58:26.25 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @15:58:26.43 Elbow.seek FlushPuck::Garage @15:58:28.29 Elbow.reconfigure :loose @15:58:28.39 Forearm.seek :garage @15:58:29.42 Hand.close @15:58:30.15 Forearm.seek :retract @15:58:31.60 Elbow.reconfigure :tight @15:58:31.65 Elbow.seek CC @15:58:34.68 Elbow.reconfigure :loose @15:58:34.79 Forearm.reconfigure :tight @15:58:34.84 Forearm.seek CC @15:58:36.08 CC.seek :closed @15:58:40.61 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.41 @15:58:40.68 Hand.open @15:58:41.34 Forearm.reconfigure :loose @15:58:41.39 Forearm.seek :retract @15:58:42.33 PSV.dial PSR @15:58:43.71 Pulling 1.5ml of PSR.flush @15:58:43.72 PSR.select :flush @15:58:43.84 PS.seek 1.5ml @15:58:59.92 delay 5 seconds @15:59:04.94 PSV.dial :waste1 @15:59:05.43 PS.seek 0ml @15:59:21.58 PSV.dial PSR @15:59:22.05 PSR.select :air @15:59:22.17 PS.seek 2.5ml @15:59:48.55 PRV.dial CBV,avoiding: :ab2 @15:59:48.58
CBV.dial PRV @15:59:50.31 PSV.dial :mixloop @15:59:51.47 CTV.dial :air,avoiding: :intake @15:59:51.65 PTV.dial :PRVmixing @15:59:52.98 PS.seek 0ml @16:00:19.36 PSV.dial :air @16:00:19.84 PS.seek 2.5ml @16:00:46.14 PSV.dial :mixloop @16:00:46.62 PS.seek 0ml @16:01:12.95 PSV.dial :air @16:01:13.43 PS.seek 2.5ml @16:01:40.26 PSV.dial :mixloop @16:01:40.74 PS.seek 0ml @16:02:07.05 Garaging FlushPuck in Collection Clamp @16:02:07.12 Elbow.reconfigure :tight @16:02:07.17 Elbow.seek CC @16:02:07.29 Elbow.reconfigure :loose @16:02:07.40 Forearm.seek CC @16:02:08.35 Hand.close @16:02:09.03 CC.seek :open @16:02:13.69 Forearm.seek :retract @16:02:14.64 Elbow.reconfigure :tight @16:02:14.69 Elbow.seek :garage @16:02:17.66 Elbow.reconfigure :loose @16:02:17.76 Forearm.reconfigure :tight @16:02:17.81 Forearm.seek :garage @16:02:18.86 Hand.open @16:02:19.59 Forearm.reconfigure :loose @16:02:19.64 Forearm.seek :retract @16:02:20.62 PSV.dial :mixloop @16:02:20.70 PTV.dial :PRVmixing @16:02:20.78 PRV.dial CBV @16:02:20.81
CBV.dial PRV @16:02:20.99 CTV.dial :air,avoiding: :intake @16:02:21.01 Loading lyfil puck from tube 3 into Collection Clamp @16:02:21.24 SC.reconfigure :tight @16:02:21.29 SC.seek 3 @16:02:24.86 SC.configure :limp @16:02:25.08 Elbow.reconfigure :tight @16:02:25.13 Elbow.seek 3 @16:02:27.07 Forearm.reconfigure :tight @16:02:27.12 Forearm.seek 3 + 450 counts @16:02:28.18 SE.reconfigure :pick @16:02:28.65 SE.seek :beamBreak @16:02:29.93 SE.configure :default @16:02:30.09 Elbow.seek 3 @16:02:30.28 Forearm.seek 3 + 300 counts @16:02:30.53 Forearm.reconfigure :loose @16:02:30.57 Hand.close @16:02:31.17 SE.seek :down @16:02:31.37
Forearm.seek :retract @16:02:32.07 SE.stop @16:02:32.33
Elbow.seek CC @16:02:33.94 Elbow.reconfigure :loose @16:02:34.05 Forearm.reconfigure :tight @16:02:34.10 Forearm.seek CC @16:02:35.29 CC.seek :closed @16:02:39.82 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.15 @16:02:39.83 Hand.open @16:02:40.47 Forearm.reconfigure :loose @16:02:40.52 Forearm.seek :retract @16:02:41.50 CTV.dial CSV,avoiding: :intake @16:02:42.85 CSV.dial CTV @16:02:43.18 PS.seek 2.5ml @16:03:09.70 CS.seek 4.5ml @16:03:24.63 delay 8 seconds @16:03:32.65 PSV.dial :air @16:03:33.11 delay 2 seconds @16:03:35.51 PSV.dial :waste2 @16:03:36.44 PS.seek 0ml @16:04:02.79 CBV.dial between PRV and waste1 @16:04:03.13 CSV.dial :air @16:04:03.60 CS.seek 10ml @16:04:18.53 CSV.dial CTV @16:04:18.99 CS.seek 0ml @16:04:45.37 CBV.dial :waste1 @16:04:45.69 delay 5 seconds @16:04:50.71 CTV.dial :air,avoiding: :intake @16:04:52.12 Storing lyfil puck in Collection Clamp to tube 2 @16:04:52.30 SC.reconfigure :tight @16:04:52.35 SC.seek 2 @16:04:55.89 SC.configure :limp @16:04:56.11 Elbow.reconfigure :tight @16:04:56.15 Elbow.seek 2 @16:04:57.93 Forearm.reconfigure :tight @16:04:57.97 Forearm.seek 2 + 450 counts @16:04:59.04 SE.reconfigure :pick @16:04:59.10 SE.seek :beamBreak @16:05:01.99 SE.configure :default @16:05:02.15 Elbow.reconfigure :limp @16:05:02.20 Forearm.reconfigure :loose @16:05:02.25 Forearm.seek 2 @16:05:02.52 Elbow.hold @16:05:02.59 Elbow.reconfigure :tight @16:05:02.69 SE.seek 3.16 Pucks below Top Plate @16:05:03.15 Forearm.force -20 @16:05:03.19 Forearm.seek 2 + 291 counts @16:05:03.58 Forearm.reconfigure :tight @16:05:03.63 Forearm.seek 2 + 450 counts @16:05:04.38 SE.seek 4.16 Pucks below Top Plate @16:05:04.88 Forearm.reconfigure :loose @16:05:04.93 Forearm.seek :retract @16:05:05.89 Elbow.seek CC @16:05:07.58 Elbow.reconfigure :loose @16:05:07.68 Forearm.seek CC @16:05:08.73 Hand.close @16:05:09.40 CC.seek :open @16:05:14.14 Forearm.seek :retract @16:05:15.11 Elbow.reconfigure :tight @16:05:15.15 Elbow.seek 2 @16:05:16.93 Elbow.reconfigure :limp @16:05:16.99 Forearm.seek 2 @16:05:17.99 Elbow.hold @16:05:18.06 Elbow.reconfigure :tight @16:05:18.11 Hand.open @16:05:18.89 Forearm.force -20 @16:05:18.94 Forearm.seek 2 + 317 counts @16:05:19.30 Forearm.reconfigure :tight @16:05:19.35 Forearm.seek 2 + 450 counts @16:05:19.68 SE.seek :down @16:05:20.82
Forearm.reconfigure :loose @16:05:20.87 Forearm.seek :retract @16:05:22.09 SE.stop @16:05:22.14
PSV.dial :air @16:05:22.88 PS.seek 2.5ml @16:05:49.70 delay 1 second @16:05:50.71 PSV.dial :mixloop @16:05:51.17 delay 1 second @16:05:52.18 PSV.dial :air @16:05:52.65 delay 1 second @16:05:53.67 PSV.dial :mixloop @16:05:54.14 delay 1 second @16:05:55.15 PSV.dial :air @16:05:55.62 delay 1 second @16:05:56.64 PS.seek 0ml @16:06:23.14 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @16:06:23.32 Elbow.seek FlushPuck::Garage @16:06:25.16 Elbow.reconfigure :loose @16:06:25.26 Forearm.seek :garage @16:06:26.29 Hand.close @16:06:27.02 Forearm.seek :retract @16:06:28.06 Elbow.reconfigure :tight @16:06:28.11 Elbow.seek PC @16:06:29.37 Elbow.reconfigure :loose @16:06:29.48 Forearm.reconfigure :tight @16:06:29.53 Forearm.seek PC @16:06:30.72 PC.seek :closed @16:06:35.38 Processing Clamp onSpring/offSpring for 16/26 samples, on/off torque 1.62 @16:06:35.45 Hand.open @16:06:36.09 Forearm.reconfigure :loose @16:06:36.14 Forearm.seek :retract @16:06:37.16 PRV.dial :air @16:06:37.63 PSV.dial :mixloop @16:06:38.63 PS.seek 0.3ml @16:06:42.70 PSV.dial PSR @16:06:43.75 Pulling 0.4ml of PSR.air @16:06:43.76 PSR.select :air @16:06:43.78 PS.seek 0.7ml @16:06:48.77 delay 3 seconds @16:06:51.78 PSR.select :air @16:06:51.81 PS.seek 2.5ml @16:07:11.01 delay 3 seconds @16:07:14.03 PSV.dial :mixloop @16:07:15.07 PTV.dial :mixing @16:07:15.84 PBV.dial :waste2 @16:07:16.78 PS.seek 0ml @16:07:43.14 Garaging FlushPuck in Processing Clamp @16:07:43.21 Elbow.reconfigure :tight @16:07:43.26 Elbow.seek PC @16:07:43.37 Elbow.reconfigure :loose @16:07:43.48 Forearm.seek PC @16:07:44.43 Hand.close @16:07:45.03 PC.seek :open @16:07:49.78 Forearm.seek :retract @16:07:50.76 Elbow.reconfigure :tight @16:07:50.81 Elbow.seek :garage @16:07:52.17 Elbow.reconfigure :loose @16:07:52.27 Forearm.reconfigure :tight @16:07:52.32 Forearm.seek :garage @16:07:53.40 Hand.open @16:07:54.09 Forearm.reconfigure :loose @16:07:54.13 Forearm.seek :retract @16:07:55.10 Loading da puck from tube 3 into Processing Clamp @16:07:55.34 SC.reconfigure :tight @16:07:55.39 SC.seek 3 @16:07:58.88 SC.configure :limp @16:07:59.09 Elbow.reconfigure :tight @16:07:59.14 Elbow.seek 3 @16:08:01.38 Forearm.reconfigure :tight @16:08:01.50 Forearm.seek 3 + 450 counts @16:08:02.51 SE.reconfigure :pick @16:08:02.56 SE.seek :beamBreak @16:08:03.87 SE.configure :default @16:08:04.03 Elbow.seek 3 @16:08:04.22 Forearm.seek 3 + 300 counts @16:08:04.46 Forearm.reconfigure :loose @16:08:04.51 Hand.close @16:08:05.16 SE.seek :down @16:08:05.37
Forearm.seek :retract @16:08:06.11 SE.stop @16:08:06.35
Elbow.seek PC @16:08:08.89 Elbow.reconfigure :loose @16:08:08.99 Forearm.reconfigure :tight @16:08:09.04 Forearm.seek PC @16:08:10.26 PC.seek :closed @16:08:14.89 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.64 @16:08:14.90 Hand.open @16:08:15.59 Forearm.reconfigure :loose @16:08:15.63 Forearm.seek :retract @16:08:16.66 Heating Puck.from 3, :da in Processing Clamp to 28C for 2:00:00 @16:08:16.68 PH.seek 28C,5:00 @16:08:55.19 PH.hold 2:00:00,28C @16:08:55.24 delay 1:30 @16:10:25.28 PSR.select :air @16:10:25.30 PSV.dial PSR @16:10:26.35 Pulling 0.5ml of PSR.wash @16:10:26.36 PSR.select :wash @16:10:26.48 PS.seek 0.5ml @16:10:32.35 delay 5 seconds @16:10:37.37 PSR.select :air @16:10:37.49 PS.seek 2ml @16:10:53.72 PTV.dial :puck @16:10:54.47 PSV.dial PTV @16:10:55.24 PBV.dial :waste1 @16:10:56.64 PS.seek 1.5ml @16:11:02.57 delay 3 seconds @16:11:05.59 PBV.dial between PSV and waste1 @16:11:05.93 PS.seek 0ml @16:11:22.07 delay 5:00 @16:16:22.09 recoverPSRreagent08 @16:16:22.10 PTV.dial :puck @16:16:22.17 PBV.dial :waste1 @16:16:22.55 delay 30 seconds @16:16:52.57 PBV.dial between PSV and waste1 @16:16:52.95 PSR.select :air @16:16:52.97 PSV.dial PSR @16:16:54.18 PS.reconfigure :fast @16:16:54.23 PS.seek 2.5ml @16:17:18.15 PSV.dial PTV @16:17:18.92 delay 5 seconds @16:17:23.94 PSV.dial PSR @16:17:24.72 delay 5 seconds @16:17:29.74 PSV.dial PTV @16:17:30.50 delay 5 seconds @16:17:35.52 PSV.dial :air @16:17:36.39 PS.seek 0ml @16:17:59.75 PTV.dial :PRVmixing,via: PRV @16:18:01.07 PSV.dial :mixloop @16:18:01.53 PRV.dial :ab1 @16:18:02.28 PS.reconfigure :slow @16:18:02.33 PS.seek 0.15ml @16:18:05.97 delay 2 seconds @16:18:07.99 CBV.dial PRV @16:18:08.01
PRV.dial CBV @16:18:08.60 PS.seek 0.35ml @16:18:13.18 delay 2 seconds @16:18:15.20 CBV.dial between PRV and PSV @16:18:15.52 PRV.dial :air @16:18:15.97 PS.seek 0.9ml @16:18:27.71 PRV.dial :waste2 @16:18:28.47 PS.seek 0.5ml @16:18:37.22 addPMLReagent08 @16:18:37.23 PSV.dial :air @16:18:37.70 PS.reconfigure :default @16:18:37.75 PS.seek 2ml @16:18:53.90 PSV.dial :PML @16:18:54.37 PTV.dial :mixing @16:18:55.12 PBV.dial :waste1 @16:18:55.56 PS.seek 1.85ml @16:18:57.87 PBV.dial between PSV and waste1 @16:18:58.25 PS.seek 0ml @16:19:17.97 delay 2 seconds @16:19:19.98 Beginning samplePassC @16:19:20.00 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @16:19:20.18 Elbow.reconfigure :tight @16:19:20.23 Elbow.seek FlushPuck::Garage @16:19:21.60 Elbow.reconfigure :loose @16:19:21.70 Forearm.seek :garage @16:19:22.67 Hand.close @16:19:23.82 Forearm.seek :retract @16:19:24.81 Elbow.reconfigure :tight @16:19:24.86 Elbow.seek CC @16:19:27.71 Elbow.reconfigure :loose @16:19:27.82 Forearm.reconfigure :tight @16:19:27.87 Forearm.seek CC @16:19:29.14 CC.seek :closed @16:19:33.68 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.47 @16:19:33.75 Hand.open @16:19:34.45 Forearm.reconfigure :loose @16:19:34.50 Forearm.seek :retract @16:19:35.47 PSV.dial PSR @16:19:36.53 Pulling 1.5ml of PSR.flush @16:19:36.54 PSR.select :flush @16:19:36.66 PS.seek 1.5ml @16:19:52.78 delay 5 seconds @16:19:57.80 PSV.dial :waste1 @16:19:58.27 PS.seek 0ml @16:20:14.46 PSV.dial PSR @16:20:14.93 PSR.select :air @16:20:15.05 PS.seek 2.5ml @16:20:41.50 PRV.dial CBV,avoiding: :ab2 @16:20:41.53
CBV.dial PRV @16:20:43.15 PSV.dial :mixloop @16:20:44.20 CTV.dial :air,avoiding: :intake @16:20:44.28 PTV.dial :PRVmixing @16:20:45.05 PS.seek 0ml @16:21:11.40 PSV.dial :air @16:21:11.98 PS.seek 2.5ml @16:21:38.75 PSV.dial :mixloop @16:21:39.28 PS.seek 0ml @16:22:05.55 PSV.dial :air @16:22:06.03 PS.seek 2.5ml @16:22:32.37 PSV.dial :mixloop @16:22:32.84 PS.seek 0ml @16:22:59.24 Garaging FlushPuck in Collection Clamp @16:22:59.32 Elbow.reconfigure :tight @16:22:59.36 Elbow.seek CC @16:22:59.61 Elbow.reconfigure :loose @16:22:59.72 Forearm.seek CC @16:23:00.74 Hand.close @16:23:01.39 CC.seek :open @16:23:06.04 Forearm.seek :retract @16:23:07.01 Elbow.reconfigure :tight @16:23:07.06 Elbow.seek :garage @16:23:09.97 Elbow.reconfigure :loose @16:23:10.07 Forearm.reconfigure :tight @16:23:10.12 Forearm.seek :garage @16:23:11.20 Hand.open @16:23:11.82 Forearm.reconfigure :loose @16:23:11.87 Forearm.seek :retract @16:23:12.86 delayUntil "16:27:19.97" @16:23:12.88 Whole Cell Archive sampling 1000ml @16:23:12.90 Loading wcr puck from tube 3 into Collection Clamp @16:23:13.38 Elbow.reconfigure :tight @16:23:13.43 Elbow.seek 3 @16:23:15.38 Forearm.reconfigure :tight @16:23:15.43 Forearm.seek 3 + 450 counts @16:23:16.90 SE.reconfigure :pick @16:23:16.97 SE.seek :beamBreak @16:23:18.36 SE.configure :default @16:23:18.53 Elbow.seek 3 @16:23:18.72 Forearm.seek 3 + 300 counts @16:23:18.98 Forearm.reconfigure :loose @16:23:19.03 Hand.close @16:23:19.65 SE.seek :down @16:23:19.86 Forearm.seek :retract @16:23:20.69 SE.stop @16:23:20.79 Elbow.seek CC @16:23:22.41 Elbow.reconfigure :loose @16:23:22.52 Forearm.reconfigure :tight @16:23:22.57 Forearm.seek CC @16:23:23.87 CC.seek :closed @16:23:28.39 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.30 @16:23:28.41 Hand.open @16:23:29.07 Forearm.reconfigure :loose @16:23:29.12 Forearm.seek :retract @16:23:30.12 collectSample 1000ml w/bubble point of 35psi @16:23:30.14 Priming 7ml @16:23:30.15 Intake.select :close @16:23:30.16 Exhaust.select :close @16:23:30.17 SS.hold @16:23:30.83 Calibrating pressure sensors @16:23:30.84 CTV.dial :air @16:23:30.92 SSV.dial :spit,avoiding: :exhaust @16:23:30.95 SS.reconfigure :fast @16:23:31.00 SS.seek 5ml @16:23:33.64 SSV.dial CBV @16:23:33.67 CBV.dial SSV @16:23:35.06 delay 3.8 seconds @16:23:39.00 Sensor limits: Intake max 111.4psia; Outlet max 103.6psia @16:23:39.01 SSV.dial :spit @16:23:40.37 SS.seek 0ml @16:23:43.14 SSV.dial CBV @16:23:44.49 CTV.dial :intake @16:23:45.25 SSV.dial CBV @16:23:45.28 CBV.dial SSV @16:23:45.50 Allowing 40s for 7.0ml of remaining 7.0ml @16:23:45.51 Intake.select :open @16:23:47.68 Intake @14.5psia, vacuum from 9.0 to 12.0psi @16:23:47.70 SS.reconfigure :inhaling 2.20ml/s @16:23:47.75 SS.seek 7ml @16:23:53.98 Stroke completed -- waiting for pressure to equalize ... @16:23:53.99 SS.reconfigure :exhale @16:23:54.04 SS.hold @16:23:54.40 Intake.select :close @16:23:54.56 SSV.dial :exhaust @16:23:55.34 Exhaust.select :open @16:23:55.46 SS.seek 0ml @16:23:59.50 Exhaust.select :close @16:23:59.61 Sampling 1000.0ml @16:23:59.62 Intake.select :close @16:23:59.63 Exhaust.select :close @16:23:59.64 SS.reconfigure :default @16:23:59.69 SS.hold @16:23:59.90 CTV.dial :intake @16:24:00.08 SSV.dial CBV @16:24:00.12 CBV.dial SSV @16:24:00.89 Allowing 130s for 25.0ml of remaining 1000.0ml @16:24:00.90 Intake.select :open @16:24:03.08 Intake @15.3psia, vacuum from 9.8 to 12.8psi @16:24:03.10 SS.reconfigure :inhaling 2.20ml/s @16:24:03.15 SS.seek 25ml @16:24:18.00 Stroke completed -- waiting for pressure to equalize ... @16:24:18.01 SS.reconfigure :exhale @16:24:18.06 SS.hold @16:24:49.01 Intake.select :close @16:24:49.56 SSV.dial :exhaust @16:24:50.40 Exhaust.select :open @16:24:50.52 SS.seek 0ml @16:24:59.15 Exhaust.select :close @16:24:59.27 SSV.dial CBV @16:24:59.30 CBV.dial SSV @16:25:00.08 Allowing 130s for 25.0ml of remaining 975.0ml @16:25:00.09 Intake.select :open @16:25:02.26 Intake @15.5psia, vacuum from 10.0 to 13.0psi @16:25:02.28 SS.reconfigure :inhaling 2.20ml/s @16:25:02.33 SS.seek 25ml @16:25:09.97 Vacuum too high -- waiting up to 40 seconds @16:25:09.99 SS.reconfigure :exhale @16:25:10.05 SS.hold @16:25:16.04 Intake @15.3psia, vacuum from 9.8 to 12.8psi @16:25:16.06 SS.reconfigure :inhaling 1.76ml/s @16:25:16.11 SS.seek 25ml @16:25:23.63 Vacuum too high -- waiting up to 40 seconds @16:25:23.65 SS.reconfigure :exhale @16:25:23.70 SS.hold @16:25:30.67 Intake @15.9psia, vacuum from 10.0 to 13.0psi @16:25:30.69 SS.reconfigure :inhaling 1.41ml/s @16:25:30.74 SS.seek 25ml @16:25:33.24 Stroke completed -- waiting for pressure to equalize ... @16:25:33.26 SS.reconfigure :exhale @16:25:33.31 SS.hold @16:25:43.60 Intake.select :close @16:25:43.77 SSV.dial :exhaust @16:25:44.54 Exhaust.select :open @16:25:44.67 SS.seek 0ml @16:25:53.45 Exhaust.select :close @16:25:53.56 SSV.dial CBV @16:25:53.60 CBV.dial SSV @16:25:54.34 Allowing 130s for 25.0ml of remaining 950.0ml @16:25:54.36 Intake.select :open @16:25:56.52 Intake @15.6psia, vacuum from 10.0 to 13.0psi @16:25:56.54 SS.reconfigure :inhaling 1.41ml/s @16:25:56.59 SS.seek 25ml @16:26:11.75 Vacuum too high -- waiting up to 40 seconds @16:26:11.77 SS.reconfigure :exhale @16:26:11.83 SS.hold @16:26:19.51 SS.reconfigure :inhaling 1.13ml/s @16:26:19.56 SS.seek 25ml @16:26:27.15 Stroke completed -- waiting for pressure to equalize ... @16:26:27.16 SS.reconfigure :exhale @16:26:27.21 SS.hold @16:26:38.65 Intake.select :close @16:26:38.81 SSV.dial :exhaust @16:26:39.57 Exhaust.select :open @16:26:39.69 SS.seek 0ml @16:26:48.93 Exhaust.select :close @16:26:49.05 SSV.dial CBV @16:26:49.07 CBV.dial SSV @16:26:49.84 Allowing 130s for 25.0ml of remaining 925.0ml @16:26:49.85 Intake.select :open @16:26:52.02 Intake @15.5psia, vacuum from 10.0 to 13.0psi @16:26:52.04 SS.reconfigure :inhaling 1.13ml/s @16:26:52.09 SS.seek 25ml @16:27:16.02 Stroke completed -- waiting for pressure to equalize ... @16:27:16.04 SS.reconfigure :exhale @16:27:16.09 SS.hold @16:27:19.98 recoverPRVreagent08 @16:27:20.00 PTV.dial :mixing @16:27:20.75 PBV.dial :waste1 @16:27:21.14 delay 30 seconds @16:27:32.75 Intake.select :close @16:27:32.91 SSV.dial :exhaust @16:27:33.68 Exhaust.select :open @16:27:33.80 SS.seek 0ml @16:27:42.49 Exhaust.select :close @16:27:42.61 SSV.dial CBV @16:27:42.64 CBV.dial SSV @16:27:43.42 Allowing 130s for 25.0ml of remaining 900.0ml @16:27:43.43 Intake.select :open @16:27:45.60 Intake @15.4psia, vacuum from 9.9 to 12.9psi @16:27:45.62 SS.reconfigure :inhaling 1.13ml/s @16:27:45.67 SS.seek 25ml @16:27:51.16 PBV.dial between PSV and waste1 @16:27:51.53 PSV.dial :air @16:27:52.15 PS.reconfigure :fast @16:27:52.20 PS.seek 2.5ml @16:28:10.68 Stroke completed -- waiting for pressure to equalize ... @16:28:10.70 SS.reconfigure :exhale @16:28:10.75 SS.hold @16:28:16.36 PSV.dial :mixloop @16:28:16.86 delay 2 seconds @16:28:18.88 PSV.dial :air @16:28:19.35 delay 2 seconds @16:28:21.37 PSV.dial :mixloop @16:28:21.85 delay 2 seconds @16:28:23.89 PSV.dial :air @16:28:24.37 PS.seek 0ml @16:28:25.15 Intake.select :close @16:28:25.31 SSV.dial :exhaust @16:28:26.07 Exhaust.select :open @16:28:26.19 SS.seek 0ml @16:28:34.98 Exhaust.select :close @16:28:35.49 SSV.dial CBV @16:28:35.52 CBV.dial SSV @16:28:36.31 Allowing 130s for 25.0ml of remaining 875.0ml @16:28:36.32 Intake.select :open @16:28:38.49 Intake @15.5psia, vacuum from 10.0 to 13.0psi @16:28:38.51 SS.reconfigure :inhaling 1.13ml/s @16:28:38.56 SS.seek 25ml @16:28:48.30 washCycleDA #1 of 2 @16:28:48.31 PSR.select :air @16:28:48.33 PSV.dial PSR @16:28:49.67 Pulling 0.5ml of PSR.wash @16:28:49.68 PSR.select :wash @16:28:49.80 PS.reconfigure :default @16:28:49.85 PS.seek 0.5ml @16:28:55.86 delay 5 seconds @16:29:00.88 PSR.select :air @16:29:01.00 PS.seek 2ml @16:29:03.22 Vacuum too high -- waiting up to 40 seconds @16:29:03.24 SS.reconfigure :exhale @16:29:03.29 SS.hold @16:29:10.89 Intake @15.2psia, vacuum from 9.7 to 12.7psi @16:29:10.91 SS.reconfigure :inhaling 0.91ml/s @16:29:10.96 SS.seek 25ml @16:29:11.13 Stroke completed -- waiting for pressure to equalize ... @16:29:11.14 SS.reconfigure :exhale @16:29:11.19 SS.hold @16:29:17.23 PTV.dial :puck @16:29:18.00 PSV.dial PTV @16:29:18.03 Intake.select :close @16:29:18.23 SSV.dial :exhaust @16:29:18.80 PBV.dial :waste1 @16:29:18.99 Exhaust.select :open @16:29:19.12 SS.seek 0ml @16:29:19.25 PS.seek 1.5ml @16:29:25.14 delay 3 seconds @16:29:28.15 Exhaust.select :close @16:29:28.20 PBV.dial between PSV and waste1 @16:29:28.26 SSV.dial CBV @16:29:28.28 CBV.dial SSV @16:29:28.58 PS.seek 0ml @16:29:29.06 Allowing 130s for 25.0ml of remaining 850.0ml @16:29:29.07 Intake.select :open @16:29:31.23 Intake @15.2psia, vacuum from 9.7 to 12.7psi @16:29:31.25 SS.reconfigure :inhaling 0.91ml/s @16:29:31.30 SS.seek 25ml @16:29:45.02 delay 2:00 @16:30:00.40 Stroke completed -- waiting for pressure to equalize ... @16:30:00.41 SS.reconfigure :exhale @16:30:00.46 SS.hold @16:30:13.40 Intake.select :close @16:30:13.56 SSV.dial :exhaust @16:30:14.35 Exhaust.select :open @16:30:14.47 SS.seek 0ml @16:30:23.43 Exhaust.select :close @16:30:23.54 SSV.dial CBV @16:30:23.56 CBV.dial SSV @16:30:24.35 Allowing 130s for 25.0ml of remaining 825.0ml @16:30:24.37 Intake.select :open @16:30:26.53 Intake @15.0psia, vacuum from 9.5 to 12.5psi @16:30:26.55 SS.reconfigure :inhaling 0.91ml/s @16:30:26.60 SS.seek 25ml @16:30:56.24 Stroke completed -- waiting for pressure to equalize ... @16:30:56.25 SS.reconfigure :exhale @16:30:56.30 SS.hold @16:31:10.93 Intake.select :close @16:31:11.09 SSV.dial :exhaust @16:31:11.87 Exhaust.select :open @16:31:11.99 SS.seek 0ml @16:31:21.04 Exhaust.select :close @16:31:21.15 SSV.dial CBV @16:31:21.18 CBV.dial SSV @16:31:21.96 Allowing 130s for 25.0ml of remaining 800.0ml @16:31:21.97 Intake.select :open @16:31:24.14 Intake @15.0psia, vacuum from 9.5 to 12.5psi @16:31:24.16 SS.reconfigure :inhaling 0.91ml/s @16:31:24.21 SS.seek 25ml @16:31:45.04 recoverPSRreagent08 @16:31:45.05 PTV.dial :puck @16:31:45.07 PBV.dial :waste1 @16:31:45.44 delay 30 seconds @16:31:53.50 Stroke completed -- waiting for pressure to equalize ... @16:31:53.52 SS.reconfigure :exhale @16:31:53.57 SS.hold @16:32:10.33 Intake.select :close @16:32:10.49 SSV.dial :exhaust @16:32:11.25 Exhaust.select :open @16:32:11.37 SS.seek 0ml @16:32:15.45 PBV.dial between PSV and waste1 @16:32:15.84 PSR.select :air @16:32:15.86 PSV.dial PSR @16:32:16.63 PS.reconfigure :fast @16:32:16.68 PS.seek 2.5ml @16:32:20.38 Exhaust.select :close @16:32:20.49 SSV.dial CBV @16:32:20.52 CBV.dial SSV @16:32:21.29 Allowing 130s for 25.0ml of remaining 775.0ml @16:32:21.30 Intake.select :open @16:32:23.47 Intake @14.9psia, vacuum from 9.4 to 12.4psi @16:32:23.49 SS.reconfigure :inhaling 0.91ml/s @16:32:23.54 SS.seek 25ml @16:32:41.36 PSV.dial PTV @16:32:42.33 delay 5 seconds @16:32:47.35 PSV.dial PSR @16:32:48.09 delay 5 seconds @16:32:51.16 Vacuum too high -- waiting up to 40 seconds @16:32:51.17 SS.reconfigure :exhale @16:32:51.23 SS.hold @16:32:53.11 PSV.dial PTV @16:32:53.87 delay 5 seconds @16:32:58.89 PSV.dial :air @16:32:59.66 PS.seek 0ml @16:33:02.63 Intake @15.3psia, vacuum from 9.8 to 12.8psi @16:33:02.65 SS.reconfigure :inhaling 0.73ml/s @16:33:02.70 SS.seek 25ml @16:33:05.34 Stroke completed -- waiting for pressure to equalize ... @16:33:05.36 SS.reconfigure :exhale @16:33:05.41 SS.hold @16:33:12.17 Intake.select :close @16:33:12.33 SSV.dial :exhaust @16:33:13.10 Exhaust.select :open @16:33:13.22 SS.seek 0ml @16:33:21.99 Exhaust.select :close @16:33:22.10 SSV.dial CBV @16:33:22.12 CBV.dial SSV @16:33:22.89 Allowing 130s for 25.0ml of remaining 750.0ml @16:33:22.91 Intake.select :open @16:33:23.33 washCycleDA #2 of 2 @16:33:23.34 PSR.select :air @16:33:23.35 PSV.dial PSR @16:33:24.70 Pulling 0.5ml of PSR.wash @16:33:24.71 PSR.select :wash @16:33:24.83 PS.reconfigure :default @16:33:24.88 PS.seek 0.5ml @16:33:25.08 Intake @15.0psia, vacuum from 9.5 to 12.5psi @16:33:25.10 SS.reconfigure :inhaling 0.73ml/s @16:33:25.15 SS.seek 25ml @16:33:30.79 delay 5 seconds @16:33:35.81 PSR.select :air @16:33:35.93 PS.seek 2ml @16:33:52.10 PTV.dial :puck @16:33:52.12 PSV.dial PTV @16:33:52.87 PBV.dial :waste1 @16:33:53.31 PS.seek 1.5ml @16:33:59.23 delay 3 seconds @16:34:01.41 Stroke completed -- waiting for pressure to equalize ... @16:34:01.42 SS.reconfigure :exhale @16:34:01.47 SS.hold @16:34:02.55 PBV.dial between PSV and waste1 @16:34:02.92 PS.seek 0ml @16:34:18.17 Intake.select :close @16:34:18.33 SSV.dial :exhaust @16:34:19.11 Exhaust.select :open @16:34:19.19 delay 2:00 @16:34:19.23 SS.seek 0ml @16:34:28.38 Exhaust.select :close @16:34:28.49 SSV.dial CBV @16:34:28.52 CBV.dial SSV @16:34:29.29 Allowing 130s for 25.0ml of remaining 725.0ml @16:34:29.30 Intake.select :open @16:34:31.47 Intake @14.8psia, vacuum from 9.3 to 12.3psi @16:34:31.49 SS.reconfigure :inhaling 0.73ml/s @16:34:31.54 SS.seek 25ml @16:35:03.93 Vacuum too high -- waiting up to 40 seconds @16:35:03.95 SS.reconfigure :exhale @16:35:04.39 SS.hold @16:35:14.42 Intake @15.2psia, vacuum from 9.7 to 12.7psi @16:35:14.44 SS.reconfigure :inhaling 0.59ml/s @16:35:14.49 SS.seek 25ml @16:35:18.67 Stroke completed -- waiting for pressure to equalize ... @16:35:18.68 SS.reconfigure :exhale @16:35:18.73 SS.hold @16:35:27.33 Intake.select :close @16:35:27.49 SSV.dial :exhaust @16:35:28.28 Exhaust.select :open @16:35:28.40 SS.seek 0ml @16:35:37.42 Exhaust.select :close @16:35:37.54 SSV.dial CBV @16:35:37.57 CBV.dial SSV @16:35:38.33 Allowing 130s for 25.0ml of remaining 700.0ml @16:35:38.34 Intake.select :open @16:35:40.51 Intake @14.7psia, vacuum from 9.2 to 12.2psi @16:35:40.53 SS.reconfigure :inhaling 0.59ml/s @16:35:40.58 SS.seek 25ml @16:36:19.21 recoverPSRreagent08 @16:36:19.22 PTV.dial :puck @16:36:19.24 PBV.dial :waste1 @16:36:19.59 delay 30 seconds @16:36:24.96 Stroke completed -- waiting for pressure to equalize ... @16:36:24.97 SS.reconfigure :exhale @16:36:25.02 SS.hold @16:36:41.80 Intake.select :close @16:36:41.96 SSV.dial :exhaust @16:36:42.74 Exhaust.select :open @16:36:42.86 SS.seek 0ml @16:36:49.61 PBV.dial between PSV and waste1 @16:36:49.99 PSR.select :air @16:36:50.01 PSV.dial PSR @16:36:50.78 PS.reconfigure :fast @16:36:50.83 PS.seek 2.5ml @16:36:51.73 Exhaust.select :close @16:36:51.85 SSV.dial CBV @16:36:51.89 CBV.dial SSV @16:36:52.64 Allowing 130s for 25.0ml of remaining 675.0ml @16:36:52.65 Intake.select :open @16:36:54.83 Intake @14.7psia, vacuum from 9.2 to 12.2psi @16:36:54.85 SS.reconfigure :inhaling 0.59ml/s @16:36:54.90 SS.seek 25ml @16:37:14.97 PSV.dial PTV @16:37:15.74 delay 5 seconds @16:37:20.76 PSV.dial PSR @16:37:21.52 delay 5 seconds @16:37:26.54 PSV.dial PTV @16:37:27.30 delay 5 seconds @16:37:32.32 PSV.dial :air @16:37:33.21 PS.seek 0ml @16:37:39.78 Stroke completed -- waiting for pressure to equalize ... @16:37:39.79 SS.reconfigure :exhale @16:37:39.84 SS.hold @16:37:56.72 getPMLReagent08 @16:37:56.73 PRV.dial :air @16:37:57.21 delay 5 seconds @16:38:00.50 Intake.select :close @16:38:00.66 SSV.dial :exhaust @16:38:01.44 Exhaust.select :open @16:38:01.56 SS.seek 0ml @16:38:02.23 PTV.dial :PRVmixing @16:38:03.57 delay 5 seconds @16:38:08.59 PRV.dial :ab2 @16:38:09.05 PTV.dial :PRVmixing @16:38:09.13 PSV.dial :mixloop @16:38:09.59 PS.reconfigure :default @16:38:09.64 PS.seek 0.3ml @16:38:10.28 Exhaust.select :close @16:38:10.39 SSV.dial CBV @16:38:10.41 CBV.dial SSV @16:38:11.66 Allowing 130s for 25.0ml of remaining 650.0ml @16:38:11.67 Intake.select :open @16:38:13.61 delay 2 seconds @16:38:13.83 Intake @14.6psia, vacuum from 9.1 to 12.1psi @16:38:13.85 SS.reconfigure :inhaling 0.59ml/s @16:38:13.90 SS.seek 25ml @16:38:15.62 PRV.dial :air @16:38:16.16 PS.seek 0.55ml @16:38:19.60 addPMLReagent08 @16:38:19.61 PSV.dial :air @16:38:20.09 PS.seek 2ml @16:38:35.72 PSV.dial :PML @16:38:36.19 PTV.dial :mixing @16:38:36.97 PBV.dial :waste1 @16:38:37.41 PS.seek 1.85ml @16:38:39.85 PBV.dial between PSV and waste1 @16:38:40.21 PS.seek 0ml @16:38:48.68 Vacuum too high -- waiting up to 40 seconds @16:38:48.69 SS.reconfigure :exhale @16:38:48.75 SS.hold @16:38:59.99 delay 2 seconds @16:39:01.71 Intake @15.0psia, vacuum from 9.5 to 12.5psi @16:39:01.73 SS.reconfigure :inhaling 0.47ml/s @16:39:01.78 SS.seek 25ml @16:39:02.03 delay 8:00 @16:39:14.74 Stroke completed -- waiting for pressure to equalize ... @16:39:14.76 SS.reconfigure :exhale @16:39:14.81 SS.hold @16:39:29.20 Intake.select :close @16:39:29.36 SSV.dial :exhaust @16:39:30.12 Exhaust.select :open @16:39:30.24 SS.seek 0ml @16:39:39.89 Exhaust.select :close @16:39:40.01 SSV.dial CBV @16:39:40.03 CBV.dial SSV @16:39:40.81 Allowing 130s for 25.0ml of remaining 625.0ml @16:39:40.82 Intake.select :open @16:39:43.00 Intake @14.6psia, vacuum from 9.1 to 12.1psi @16:39:43.02 SS.reconfigure :inhaling 0.47ml/s @16:39:43.07 SS.seek 25ml @16:40:38.51 Stroke completed -- waiting for pressure to equalize ... @16:40:38.52 SS.reconfigure :exhale @16:40:38.57 SS.hold @16:41:01.04 Intake.select :close @16:41:01.20 SSV.dial :exhaust @16:41:01.98 Exhaust.select :open @16:41:02.10 SS.seek 0ml @16:41:10.84 Exhaust.select :close @16:41:10.95 SSV.dial CBV @16:41:10.97 CBV.dial SSV @16:41:11.74 Allowing 130s for 25.0ml of remaining 600.0ml @16:41:11.76 Intake.select :open @16:41:13.92 Intake @14.6psia, vacuum from 9.1 to 12.1psi @16:41:13.94 SS.reconfigure :inhaling 0.47ml/s @16:41:13.99 SS.seek 25ml @16:42:09.83 Stroke completed -- waiting for pressure to equalize ... @16:42:09.85 SS.reconfigure :exhale @16:42:09.90 SS.hold @16:42:36.82 Intake.select :close @16:42:36.98 SSV.dial :exhaust @16:42:37.75 Exhaust.select :open @16:42:37.86 SS.seek 0ml @16:42:46.62 Exhaust.select :close @16:42:46.73 SSV.dial CBV @16:42:46.76 CBV.dial SSV @16:42:47.54 Allowing 130s for 25.0ml of remaining 575.0ml @16:42:47.55 Intake.select :open @16:42:49.72 Intake @14.5psia, vacuum from 9.0 to 12.0psi @16:42:49.74 SS.reconfigure :inhaling 0.47ml/s @16:42:49.79 SS.seek 25ml @16:43:35.42 Vacuum too high -- waiting up to 40 seconds @16:43:35.44 SS.reconfigure :exhale @16:43:35.49 SS.hold @16:43:52.01 Intake @14.8psia, vacuum from 9.3 to 12.3psi @16:43:52.03 SS.reconfigure :inhaling 0.38ml/s @16:43:52.08 SS.seek 25ml @16:44:04.91 Stroke completed -- waiting for pressure to equalize ... @16:44:04.92 SS.reconfigure :exhale @16:44:04.97 SS.hold @16:44:23.33 Intake.select :close @16:44:23.49 SSV.dial :exhaust @16:44:24.27 Exhaust.select :open @16:44:24.39 SS.seek 0ml @16:44:33.38 Exhaust.select :close @16:44:33.49 SSV.dial CBV @16:44:33.51 CBV.dial SSV @16:44:34.30 Allowing 130s for 25.0ml of remaining 550.0ml @16:44:34.32 Intake.select :open @16:44:36.48 Intake @14.6psia, vacuum from 9.1 to 12.1psi @16:44:36.50 SS.reconfigure :inhaling 0.38ml/s @16:44:36.55 SS.seek 25ml @16:45:45.32 Stroke completed -- waiting for pressure to equalize ... @16:45:45.33 SS.reconfigure :exhale @16:45:45.38 SS.hold @16:46:18.56 Intake.select :close @16:46:18.72 SSV.dial :exhaust @16:46:19.50 Exhaust.select :open @16:46:19.61 SS.seek 0ml @16:46:28.42 Exhaust.select :close @16:46:28.54 SSV.dial CBV @16:46:28.58 CBV.dial SSV @16:46:29.35 Allowing 130s for 25.0ml of remaining 525.0ml @16:46:29.36 Intake.select :open @16:46:31.52 Intake @14.5psia, vacuum from 9.0 to 12.0psi @16:46:31.54 SS.reconfigure :inhaling 0.38ml/s @16:46:31.59 SS.seek 25ml @16:47:02.05 recoverPRVreagent08 @16:47:02.06 PTV.dial :mixing @16:47:02.23 PBV.dial :waste1 @16:47:02.61 delay 30 seconds @16:47:32.63 PBV.dial between PSV and waste1 @16:47:33.01 PSV.dial :air @16:47:33.61 PS.reconfigure :fast @16:47:33.66 PS.seek 2.5ml @16:47:35.01 Vacuum too high -- waiting up to 40 seconds @16:47:35.03 SS.reconfigure :exhale @16:47:35.08 SS.hold @16:47:56.18 Intake @15.1psia, vacuum from 9.6 to 12.6psi @16:47:56.20 SS.reconfigure :inhaling 0.31ml/s @16:47:56.25 SS.seek 25ml @16:47:57.90 PSV.dial :mixloop @16:47:58.35 delay 2 seconds @16:48:00.37 PSV.dial :air @16:48:00.84 delay 2 seconds @16:48:02.86 PSV.dial :mixloop @16:48:03.32 delay 2 seconds @16:48:04.01 Stroke completed -- waiting for pressure to equalize ... @16:48:04.03 SS.reconfigure :exhale @16:48:04.08 SS.hold @16:48:05.34 PSV.dial :air @16:48:05.81 PS.seek 0ml @16:48:20.72 Intake.select :close @16:48:20.88 SSV.dial :exhaust @16:48:21.64 Exhaust.select :open @16:48:21.76 SS.seek 0ml @16:48:29.43 washCycleDA #1 of 3 @16:48:29.44 PSR.select :air @16:48:29.46 PSV.dial PSR @16:48:30.78 Exhaust.select :close @16:48:30.85 Pulling 0.5ml of PSR.wash @16:48:30.86 PSR.select :wash @16:48:30.93 SSV.dial CBV @16:48:30.95 CBV.dial SSV @16:48:31.01 PS.reconfigure :default @16:48:31.06 PS.seek 0.5ml @16:48:31.72 Allowing 130s for 25.0ml of remaining 500.0ml @16:48:31.74 Intake.select :open @16:48:33.91 Intake @14.6psia, vacuum from 9.1 to 12.1psi @16:48:33.93 SS.reconfigure :inhaling 0.31ml/s @16:48:33.98 SS.seek 25ml @16:48:37.56 delay 5 seconds @16:48:42.58 PSR.select :air @16:48:42.70 PS.seek 2ml @16:48:58.83 PTV.dial :puck @16:48:59.60 PSV.dial PTV @16:49:00.35 PBV.dial :waste1 @16:49:00.79 PS.seek 1.5ml @16:49:06.80 delay 3 seconds @16:49:09.82 PBV.dial between PSV and waste1 @16:49:10.20 PS.seek 0ml @16:49:26.56 delay 2:00 @16:50:02.78 Stroke completed -- waiting for pressure to equalize ... @16:50:02.79 SS.reconfigure :exhale @16:50:02.84 SS.hold @16:50:36.03 Intake.select :close @16:50:36.19 SSV.dial :exhaust @16:50:36.96 Exhaust.select :open @16:50:37.08 SS.seek 0ml @16:50:46.15 Exhaust.select :close @16:50:46.27 SSV.dial CBV @16:50:46.31 CBV.dial SSV @16:50:47.06 Allowing 130s for 25.0ml of remaining 475.0ml @16:50:47.08 Intake.select :open @16:50:49.24 Intake @14.6psia, vacuum from 9.1 to 12.1psi @16:50:49.26 SS.reconfigure :inhaling 0.31ml/s @16:50:49.31 SS.seek 25ml @16:51:26.58 recoverPSRreagent08 @16:51:26.59 PTV.dial :puck @16:51:26.77 PBV.dial :waste1 @16:51:27.15 delay 30 seconds @16:51:57.17 PBV.dial between PSV and waste1 @16:51:57.55 PSR.select :air @16:51:57.57 PSV.dial PSR @16:51:58.34 PS.reconfigure :fast @16:51:58.39 PS.seek 2.5ml @16:52:19.84 Stroke completed -- waiting for pressure to equalize ... @16:52:19.85 SS.reconfigure :exhale @16:52:19.90 SS.hold @16:52:22.86 PSV.dial PTV @16:52:23.63 delay 5 seconds @16:52:28.65 PSV.dial PSR @16:52:29.41 delay 5 seconds @16:52:34.43 PSV.dial PTV @16:52:35.19 delay 5 seconds @16:52:40.21 PSV.dial :air @16:52:40.98 PS.seek 0ml @16:52:57.09 SS.reconfigure :default @16:52:57.14 SS.hold @16:52:57.21 Intake.select :close @16:52:57.38 ShallowSampler::Clogged in WCR -- Filtering too Slowly after 550.0ml @16:52:57.39 SSV.dial :exhaust @16:52:58.15 Exhaust.select :open @16:52:58.27 SS.reconfigure :exhale @16:52:58.32 SS.seek 0ml @16:53:04.55 washCycleDA #2 of 3 @16:53:04.56 PSR.select :air @16:53:04.58 PSV.dial PSR @16:53:05.93 Pulling 0.5ml of PSR.wash @16:53:05.94 PSR.select :wash @16:53:06.06 PS.reconfigure :default @16:53:06.11 PS.seek 0.5ml @16:53:07.45 Exhaust.select :close @16:53:07.56 Sampled 550.0ml @16:53:07.57 Drying puck before fixing for 5:00 @16:53:07.58 CBV.dial :waste2b @16:53:08.35 delay 1:00 @16:53:12.08 delay 5 seconds @16:53:17.10 PSR.select :air @16:53:17.22 PS.seek 2ml @16:53:33.36 PTV.dial :puck @16:53:33.53 PSV.dial PTV @16:53:34.28 PBV.dial :waste1 @16:53:34.72 PS.seek 1.5ml @16:53:40.67 delay 3 seconds @16:53:43.69 PBV.dial between PSV and waste1 @16:53:44.07 PS.seek 0ml @16:54:00.43 delay 2:00 @16:54:08.37 CSV.dial :air @16:54:08.83 CS.reconfigure :fast @16:54:08.89 CS.seek 10ml @16:54:33.58 Pumping wcr puck from tube 3 up to 35psi... @16:54:33.60 CBV.dial SSV @16:54:34.86 CBV.dial between CSV and waste2b @16:54:35.82 CSV.dial between air and CTV @16:54:36.15 CS.seek 7.472ml @16:54:42.86 CTV.dial CSV,avoiding: :air @16:54:42.89 CSV.dial CTV @16:54:43.74 CS.reconfigure :pressurize @16:54:43.79 CS.seek 0ml @16:55:03.42 CSV.dial :air @16:55:03.86 CS.reconfigure :fast @16:55:04.39 CS.seek 10ml @16:55:29.07 CSV.dial between air and CTV @16:55:29.52 CS.seek 4.269ml @16:55:43.30 CTV.dial CSV,avoiding: :air @16:55:43.33 CSV.dial CTV @16:55:43.82 CS.reconfigure :pressurize @16:55:43.87 CS.seek 0ml @16:55:55.32 CSV.dial :air @16:55:55.77 CS.reconfigure :fast @16:55:55.82 CS.seek 10ml @16:56:00.45 recoverPSRreagent08 @16:56:00.46 PTV.dial :puck @16:56:00.48 PBV.dial :waste1 @16:56:00.84 delay 30 seconds @16:56:20.20 CSV.dial between air and CTV @16:56:20.52 CS.seek 4.299ml @16:56:30.87 PBV.dial between PSV and waste1 @16:56:31.25 PSR.select :air @16:56:31.27 PSV.dial PSR @16:56:32.04 PS.reconfigure :fast @16:56:32.09 PS.seek 2.5ml @16:56:34.42 CTV.dial CSV,avoiding: :air @16:56:34.45 CSV.dial CTV @16:56:34.90 CS.reconfigure :pressurize @16:56:34.95 CS.seek 0ml @16:56:46.42 CSV.dial :air @16:56:46.86 CS.reconfigure :fast @16:56:46.92 CS.seek 10ml @16:56:56.20 PSV.dial PTV @16:56:56.97 delay 5 seconds @16:57:01.99 PSV.dial PSR @16:57:02.75 delay 5 seconds @16:57:07.77 PSV.dial PTV @16:57:08.53 delay 5 seconds @16:57:11.32 CSV.dial between air and CTV @16:57:11.65 CS.seek 4.36ml @16:57:13.55 PSV.dial :air @16:57:14.43 PS.seek 0ml @16:57:25.33 CTV.dial CSV,avoiding: :air @16:57:25.36 CSV.dial CTV @16:57:26.13 CS.reconfigure :pressurize @16:57:26.18 CS.seek 0ml @16:57:37.85 CSV.dial :air @16:57:37.98 washCycleDA #3 of 3 @16:57:38.00 PSR.select :air @16:57:38.01 PSV.dial PSR @16:57:38.31 CS.reconfigure :default @16:57:38.36 CS.hold @16:57:38.49 ShallowSampler::Error in MAIN -- Puck.from 3, :wcr reached only 20.6 of 35psi after pumping in 40ml of gas @16:57:38.50 Waiting to Resume from Checkpoint "Pumping wcr puck from tube 3 up to 35psi..." @16:57:38.52 BadNews.email "Puck.from 3, :wcr reached only 20.6 of 35psi after pumping in 40ml of gas",Subject: "ShallowSampler::Error in MAIN" @16:57:39.36 Pulling 0.5ml of PSR.wash @16:57:39.37 PSR.select :wash @16:57:39.49 PS.reconfigure :default @16:57:39.54 PS.seek 0.5ml @16:57:42.77 upsync @16:57:45.49 delay 5 seconds @16:57:50.51 PSR.select :air @16:57:50.64 PS.seek 2ml @16:58:06.83 PTV.dial :puck @16:58:06.84 PSV.dial PTV @16:58:07.61 PBV.dial :waste1 @16:58:08.05 PS.seek 1.5ml @16:58:13.95 delay 3 seconds @16:58:16.97 PBV.dial between PSV and waste1 @16:58:17.35 PS.seek 0ml @16:58:33.48 delay 2:00 @17:00:33.51 recoverPSRreagent08 @17:00:33.52 PTV.dial :puck @17:00:33.54 PBV.dial :waste1 @17:00:33.92 delay 30 seconds @17:01:03.95 PBV.dial between PSV and waste1 @17:01:04.32 PSR.select :air @17:01:04.34 PSV.dial PSR @17:01:05.09 PS.reconfigure :fast @17:01:05.14 PS.seek 2.5ml @17:01:29.26 PSV.dial PTV @17:01:30.03 delay 5 seconds @17:01:35.05 PSV.dial PSR @17:01:35.79 delay 5 seconds @17:01:40.81 PSV.dial PTV @17:01:41.59 delay 5 seconds @17:01:46.61 PSV.dial :air @17:01:47.36 PS.seek 0ml @17:02:10.63 Gate.power :camera,:ON @17:02:10.65 Mixing DA Substrates @17:02:10.66 PSR.select :air @17:02:10.68 PSV.dial PSR @17:02:12.11 Pulling 0.25ml of PSR.sub1 @17:02:12.13 PSR.select :sub1 @17:02:12.35 PS.reconfigure :default @17:02:12.47 PS.seek 0.25ml @17:02:16.37 delay 5 seconds @17:02:21.45 Pulling 0.25ml of PSR.sub2 @17:02:21.46 PSR.select :sub2 @17:02:21.57 PS.seek 0.5ml @17:02:24.91 delay 5 seconds @17:02:29.93 PSR.select :air @17:02:30.05 PS.seek 2ml @17:02:46.16 PTV.dial :puck @17:02:46.18 PSV.dial PTV @17:02:46.95 PBV.dial :waste1 @17:02:47.38 PS.seek 1.5ml @17:02:53.26 delay 3 seconds @17:02:56.28 PBV.dial between PSV and waste1 @17:02:56.65 PS.seek 0ml @17:03:12.79 delay 10 seconds @17:03:22.82 recoverPSRreagent08 @17:03:22.83 PTV.dial :puck @17:03:22.84 PBV.dial :waste1 @17:03:23.23 delay 30 seconds @17:03:53.25 PBV.dial between PSV and waste1 @17:03:53.63 PSR.select :air @17:03:53.65 PSV.dial PSR @17:03:54.43 PS.reconfigure :fast @17:03:54.48 PS.seek 2.5ml @17:04:18.85 PSV.dial PTV @17:04:19.62 delay 5 seconds @17:04:24.64 PSV.dial PSR @17:04:25.40 delay 5 seconds @17:04:30.42 PSV.dial PTV @17:04:31.30 delay 5 seconds @17:04:36.32 PSV.dial :air @17:04:37.23 PS.seek 0ml @17:05:01.16 Additional air clear @17:05:01.17 PSR.select :air @17:05:01.19 PSV.dial PSR @17:05:02.57 Pulling 2ml of PSR.air @17:05:02.58 PSR.select :air @17:05:02.60 PS.reconfigure :default @17:05:02.65 PS.seek 2ml @17:05:23.94 delay 5 seconds @17:05:28.96 PTV.dial :puck @17:05:28.98 PSV.dial PTV @17:05:29.86 PBV.dial :waste1 @17:05:30.30 PS.seek 1.5ml @17:05:36.35 delay 3 seconds @17:05:39.37 PBV.dial between PSV and waste1 @17:05:39.74 delay 1 second @17:05:40.76 PSV.dial :waste1 @17:05:41.83 PS.seek 0ml @17:05:58.06 Processing Puck Heater at 28.1C before imaging @17:05:58.07 PH.coast @17:05:58.17 Imaging da puck in Processing Clamp while storing it to tube 2 @17:05:58.35 SC.reconfigure :tight @17:05:58.40 SC.seek 2 @17:06:01.98 SC.configure :limp @17:06:02.20 Elbow.reconfigure :tight @17:06:02.26 Elbow.seek 2 @17:06:04.54 Forearm.reconfigure :tight @17:06:04.59 Forearm.seek 2 + 450 counts @17:06:05.57 SE.reconfigure :pick @17:06:05.63 SE.seek :beamBreak @17:06:08.49 SE.configure :default @17:06:08.65 Elbow.reconfigure :limp @17:06:08.71 Forearm.reconfigure :loose @17:06:08.76 Forearm.seek 2 @17:06:09.03 Elbow.hold @17:06:09.09 Elbow.reconfigure :tight @17:06:09.20 SE.seek 4.15 Pucks below Top Plate @17:06:09.60 Forearm.force -20 @17:06:09.64 Forearm.seek 2 + 294 counts @17:06:10.05 Forearm.reconfigure :tight @17:06:10.10 Forearm.seek 2 + 450 counts @17:06:10.34 SE.seek 5.15 Pucks below Top Plate @17:06:10.86 Forearm.reconfigure :loose @17:06:10.91 Forearm.seek :retract @17:06:11.88 Elbow.seek PC @17:06:14.38 Elbow.reconfigure :loose @17:06:14.48 Forearm.seek PC @17:06:15.46 Hand.close @17:06:16.10 PC.seek :open @17:06:20.86 Forearm.seek :retract @17:06:21.84 Elbow.reconfigure :tight @17:06:21.89 Elbow.seek 2 @17:06:24.48 Forearm.reconfigure :tight @17:06:24.53 Forearm.seek 2 + 450 counts @17:06:25.49 Hand.open @17:06:26.16 SE.seek 4.8 Pucks below Top Plate @17:06:26.43 SE.reconfigure :seal @17:06:26.48 SE.seek 3.84 Pucks below Top Plate @17:06:27.00 Lights, camera, ACTION! @17:06:27.54 PuckCamera: snap -bin=2,2 -auto /var/log/esp/da23oct1817h5ml.tif @17:06:46.53 Waiting >20 seconds for da to relinquish FlushPuck @17:06:53.23 SX HX9/16 (USB:10): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 22.364 seconds /var/log/esp/da23oct1817h5ml.tif: TIFF Upload Complete @17:06:53.27 PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da23oct1817h5ml15s.tif @17:07:08.98 SX HX9/16 (USB:10): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15 seconds /var/log/esp/midres/da23oct1817h5ml15s.tif: TIFF Upload Complete @17:07:09.01 PuckCamera: snap -bin=2,2 20.0 /var/log/esp/midres/da23oct1817h5ml20s.tif @17:07:29.63 SX HX9/16 (USB:10): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 20 seconds /var/log/esp/midres/da23oct1817h5ml20s.tif: TIFF Upload Complete @17:07:29.65 Gate.power :camera,:OFF @17:07:29.67 DAfans.email "22.364 sec auto-exposure",Subject: "da23oct1817h5ml images!" @17:07:30.14 Elbow.seek 2 @17:07:30.37 Forearm.seek 2 + 450 counts @17:07:30.47 SE.reconfigure :gently @17:07:30.52 SE.seek 4.55 Pucks below Top Plate @17:07:31.17 Elbow.reconfigure :limp @17:07:31.23 Forearm.reconfigure :loose @17:07:31.28 Forearm.seek 2 @17:07:31.54 Elbow.hold @17:07:31.60 Elbow.reconfigure :tight @17:07:31.67 SE.reconfigure :default @17:07:31.73 SE.seek 4.74 Pucks below Top Plate @17:07:32.19 Forearm.force -20 @17:07:32.24 Forearm.seek 2 + 300 counts @17:07:32.88 Forearm.reconfigure :tight @17:07:33.00 Forearm.seek 2 + 450 counts @17:07:33.40 SE.seek :down @17:07:34.67 Forearm.reconfigure :loose @17:07:34.77 Forearm.seek :retract @17:07:35.80 SE.stop @17:07:36.01 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @17:07:36.56 Elbow.seek FlushPuck::Garage @17:07:38.58 Elbow.reconfigure :loose @17:07:38.79 Forearm.seek :garage @17:07:39.80 Hand.close @17:07:41.19 Forearm.seek :retract @17:07:42.21 Elbow.reconfigure :tight @17:07:42.31 Elbow.seek PC @17:07:43.92 Elbow.reconfigure :loose @17:07:44.12 Forearm.reconfigure :tight @17:07:44.22 Forearm.seek PC @17:07:45.84 PC.seek :closed @17:07:50.72 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.62 @17:07:50.86 Hand.open @17:07:51.62 Forearm.reconfigure :loose @17:07:51.71 Forearm.seek :retract @17:07:52.74 PSR.select :air @17:07:52.78 PSV.dial PSR @17:07:53.30 Pulling 0.5ml of PSR.flush @17:07:53.32 PSR.select :flush @17:07:53.49 PS.seek 0.5ml @17:07:59.51 delay 5 seconds @17:08:02.49 upsync @17:08:04.55 PSR.select :air @17:08:04.68 PS.seek 2.5ml @17:08:25.97 delay 1 second @17:08:26.99 PRV.dial :waste2,avoiding: :ab2 @17:08:28.88 PTV.dial :PRVmixing @17:08:30.20 PSV.dial :mixloop @17:08:31.24 PS.seek :empty @17:08:57.62 PSR.select :air @17:08:57.64 PSV.dial PSR @17:08:58.69 Pulling 0.5ml of PSR.flush @17:08:58.70 PSR.select :flush @17:08:58.82 PS.seek 0.5ml @17:09:04.81 delay 5 seconds @17:09:09.83 PSR.select :air @17:09:09.95 PS.seek 2.5ml @17:09:31.16 delay 1 second @17:09:32.17 PBV.dial :waste1 @17:09:32.55 PTV.dial :mixing @17:09:33.31 PSV.dial :mixloop @17:09:34.36 PS.seek :empty @17:10:01.22 PSV.dial PSR @17:10:02.26 PSR.select :flush @17:10:02.39 PS.seek 1ml @17:10:13.78 PSR.select :air @17:10:13.90 PS.seek 2.5ml @17:10:30.06 PSV.dial PTV @17:10:30.83 PTV.dial :puck @17:10:31.58 PBV.dial :waste1 @17:10:31.66 PS.seek 0ml @17:10:58.00 PSV.dial PSR @17:10:58.76 PSR.select :flush @17:10:58.89 PS.seek 0.5ml @17:11:04.84 PSR.select :air @17:11:04.96 PS.seek 2.5ml @17:11:26.25 PSV.dial PTV @17:11:27.01 PBV.dial PSV @17:11:27.60 PS.seek 0ml @17:11:53.96 PSV.dial PBV @17:11:54.44 PS.seek 2.5ml @17:12:20.81 PSV.dial :waste1 @17:12:21.56 PS.seek 0ml @17:12:47.87 PSV.dial PSR @17:12:48.34 PSR.select :air @17:12:48.36 PS.seek 2.5ml @17:13:14.77 PSV.dial PTV @17:13:15.51 PBV.dial PSV @17:13:15.70 PS.seek 0ml @17:13:42.09 PSV.dial PBV @17:13:42.56 PS.seek 2.5ml @17:14:08.99 PSV.dial :waste1 @17:14:09.86 PS.seek 0ml @17:14:36.24 PSV.dial PSR @17:14:36.70 PSR.select :flush @17:14:36.82 PS.seek 1ml @17:14:47.93 PSR.select :air @17:14:48.05 PS.seek 2.5ml @17:15:04.21 PSV.dial PTV @17:15:04.96 PTV.dial :puck @17:15:05.15 PBV.dial :waste1 @17:15:05.70 PS.seek 0ml @17:15:32.08 PSV.dial PSR @17:15:32.85 PSR.select :flush @17:15:33.42 PS.seek 0.5ml @17:15:39.40 PSR.select :air @17:15:39.52 PS.seek 2.5ml @17:16:00.77 PSV.dial PTV @17:16:01.54 PBV.dial PSV @17:16:02.11 PS.seek 0ml @17:16:28.68 PSV.dial PBV @17:16:29.15 PS.seek 2.5ml @17:16:55.49 PSV.dial :waste1 @17:16:56.25 PS.seek 0ml @17:17:22.61 PSV.dial PSR @17:17:23.08 PSR.select :air @17:17:23.11 PS.seek 2.5ml @17:17:49.49 PSV.dial PTV @17:17:50.22 PBV.dial PSV @17:17:50.42 PS.seek 0ml @17:18:16.80 PSV.dial PBV @17:18:17.28 PS.seek 2.5ml @17:18:43.67 PSV.dial :waste1 @17:18:44.43 PS.seek 0ml @17:19:10.74 Garaging FlushPuck in Processing Clamp @17:19:10.81 Elbow.reconfigure :tight @17:19:10.86 Elbow.seek PC @17:19:10.98 Elbow.reconfigure :loose @17:19:11.09 Forearm.seek PC @17:19:12.06 Hand.close @17:19:12.72 PC.seek :open @17:19:17.48 Forearm.seek :retract @17:19:18.48 Elbow.reconfigure :tight @17:19:18.53 Elbow.seek :garage @17:19:19.83 Elbow.reconfigure :loose @17:19:19.93 Forearm.reconfigure :tight @17:19:19.98 Forearm.seek :garage @17:19:20.93 Hand.open @17:19:21.65 Forearm.reconfigure :loose @17:19:21.70 Forearm.seek :retract @16:53:39.12UTC19-Oct-23 Session Begun @16:53:55.86 -> CC Collection Clamp contains wcr puck from tube 3 @16:53:58.24 -> PC Processing Clamp open @16:54:24.50 -> Puck.count @16:54:24.59 SE.seek :down @16:54:24.68 SE.stop @16:54:24.79 SC.reconfigure :tight @16:54:24.87 SC.seek 1 @16:54:28.55 SC.configure :limp @16:54:28.79 Elbow.reconfigure :tight @16:54:28.86 Elbow.seek 1 @16:54:30.75 Forearm.reconfigure :tight @16:54:30.82 Forearm.seek 1 + 450 counts @16:54:31.90 SE.reconfigure :place @16:54:31.98 SE.seek :beamBreak @16:54:33.12 SE.configure :default @16:54:33.25 SE.seek :down @16:54:34.11 SE.stop @16:54:34.39 Forearm.reconfigure :loose @16:54:34.47 Forearm.seek :retract @16:54:35.43 SC.reconfigure :tight @16:54:35.56 SC.seek 2 @16:54:39.15 SC.configure :limp @16:54:39.33 Elbow.seek 2 @16:54:39.91 Forearm.reconfigure :tight @16:54:39.99 Forearm.seek 2 + 450 counts @16:54:41.07 SE.reconfigure :place @16:54:41.15 SE.seek :beamBreak @16:54:44.11 SE.configure :default @16:54:44.24 SE.seek :down @16:54:45.37 Forearm.reconfigure :loose @16:54:45.44 Forearm.seek :retract @16:54:46.55 SE.stop @16:54:46.62 SC.reconfigure :tight @16:54:46.68 SC.seek 3 @16:54:50.13 SC.configure :limp @16:54:50.31 Elbow.seek 3 @16:54:50.62 Forearm.reconfigure :tight @16:54:50.70 Forearm.seek 3 + 450 counts @16:54:51.71 SE.reconfigure :place @16:54:51.79 SE.seek :beamBreak @16:54:53.48 SE.configure :default @16:54:53.61 SE.seek :down @16:54:54.55 SE.stop @16:54:54.74 Forearm.reconfigure :loose @16:54:54.81 Forearm.seek :retract @16:54:55.80 SC.reconfigure :tight @16:54:55.87 SC.seek 4 @16:54:59.43 SC.configure :limp @16:54:59.61 Elbow.seek 4 @16:55:00.23 Forearm.reconfigure :tight @16:55:00.31 Forearm.seek 4 + 450 counts @16:55:01.35 SE.reconfigure :place @16:55:01.82 SE.seek :beamBreak @16:55:03.06 SE.configure :default @16:55:03.19 SE.seek :down @16:55:03.63 SE.stop @16:55:04.32 Forearm.reconfigure :loose @16:55:04.40 Forearm.seek :retract @16:55:05.37 SC.reconfigure :tight @16:55:05.44 SC.seek 5 @16:55:09.04 SC.configure :limp @16:55:09.22 Elbow.seek 5 @16:55:09.55 Forearm.reconfigure :tight @16:55:09.62 Forearm.seek 5 + 450 counts @16:55:10.72 SE.reconfigure :place @16:55:10.81 SE.seek :beamBreak @16:55:11.98 SE.configure :default @16:55:12.18 SE.seek :down @16:55:12.63 SE.stop @16:55:13.31 Forearm.reconfigure :loose @16:55:13.38 Forearm.seek :retract @16:55:14.36 SC.reconfigure :tight @16:55:14.43 SC.seek 6 @16:55:18.01 SC.configure :limp @16:55:18.18 Elbow.seek 6 @16:55:18.39 Forearm.reconfigure :tight @16:55:18.46 Forearm.seek 6 + 450 counts @16:55:19.53 SE.reconfigure :place @16:55:19.61 SE.seek :beamBreak @16:55:20.81 SE.configure :default @16:55:20.94 SE.seek :down @16:55:21.36 SE.stop @16:55:22.07 Forearm.reconfigure :loose @16:55:22.14 Forearm.seek :retract @16:55:23.12 SC.reconfigure :tight @16:55:23.19 SC.seek 7 @16:55:26.71 SC.configure :limp @16:55:26.89 Elbow.seek 7 @16:55:27.91 Forearm.reconfigure :tight @16:55:27.98 Forearm.seek 7 + 450 counts @16:55:29.00 SE.reconfigure :place @16:55:29.09 SE.seek :beamBreak @16:55:30.37 SE.configure :default @16:55:30.50 SE.seek :down @16:55:31.11 SE.stop @16:55:31.63 Forearm.reconfigure :loose @16:55:31.71 Forearm.seek :retract {1 => 33, 2 => 6, 3 => 26, 4 => 33, 5 => 33, 6 => 33, 7 => 31} @16:56:28.40 -> CC.unloadPuck 2, :WCR @16:56:28.49 Puck::Warning in dave -- Unloading Puck.from 3, :wcr to Storage Carousel @16:56:28.51 Storing WCR puck in Collection Clamp to tube 2 @16:56:28.72 SC.reconfigure :tight @16:56:28.79 SC.seek 2 @16:56:44.97 SC.configure :limp @16:56:45.14 Elbow.seek 2 @16:56:45.63 Forearm.reconfigure :tight @16:56:45.70 Forearm.seek 2 + 450 counts @16:56:46.72 SE.reconfigure :pick @16:56:46.80 SE.seek :beamBreak @16:56:49.55 SE.configure :default @16:56:49.73 Elbow.reconfigure :limp @16:56:49.80 Forearm.reconfigure :loose @16:56:49.87 Forearm.seek 2 @16:56:50.14 Elbow.hold @16:56:50.23 Elbow.reconfigure :tight @16:56:50.35 SE.seek 5.14 Pucks below Top Plate @16:56:50.79 Forearm.force -20 @16:56:50.85 Forearm.seek 2 + 298 counts @16:56:51.19 Forearm.reconfigure :tight @16:56:51.26 Forearm.seek 2 + 450 counts @16:56:51.49 SE.seek 6.14 Pucks below Top Plate @16:56:52.03 Forearm.reconfigure :loose @16:56:52.10 Forearm.seek :retract @16:56:53.02 Elbow.seek CC @16:56:54.71 Elbow.reconfigure :loose @16:56:54.83 Forearm.seek CC @16:56:55.82 Hand.close @16:56:56.44 CC.seek :open @16:57:01.28 Forearm.seek :retract @16:57:02.24 Elbow.reconfigure :tight @16:57:02.31 Elbow.seek 2 @16:57:04.10 Elbow.reconfigure :limp @16:57:04.17 Forearm.seek 2 @16:57:05.16 Elbow.hold @16:57:05.26 Elbow.reconfigure :tight @16:57:05.32 Hand.open @16:57:06.07 Forearm.force -20 @16:57:06.13 Forearm.seek 2 + 314 counts @16:57:06.52 Forearm.reconfigure :tight @16:57:06.59 Forearm.seek 2 + 450 counts @16:57:06.94 SE.seek :down @16:57:08.07 Forearm.reconfigure :loose @16:57:08.15 Forearm.seek :retract @16:57:09.25 SE.stop @16:57:17.06 -> Puck.count @16:57:17.15 SE.seek :down @16:57:17.24 SE.stop @16:57:17.35 SC.reconfigure :tight @16:57:17.42 SC.seek 1 @16:57:21.11 SC.configure :limp @16:57:21.29 Elbow.seek 1 @16:57:21.62 Forearm.reconfigure :tight @16:57:21.70 Forearm.seek 1 + 450 counts @16:57:22.76 SE.reconfigure :place @16:57:22.84 SE.seek :beamBreak @16:57:24.03 SE.configure :default @16:57:24.15 SE.seek :down @16:57:24.61 SE.stop @16:57:25.29 Forearm.reconfigure :loose @16:57:25.36 Forearm.seek :retract @16:57:26.34 SC.reconfigure :tight @16:57:26.41 SC.seek 2 @16:57:30.00 SC.configure :limp @16:57:30.18 Elbow.seek 2 @16:57:30.85 Forearm.reconfigure :tight @16:57:30.92 Forearm.seek 2 + 450 counts @16:57:32.04 SE.reconfigure :place @16:57:32.12 SE.seek :beamBreak @16:57:34.99 SE.configure :default @16:57:35.13 SE.seek :down @16:57:36.26 Forearm.reconfigure :loose @16:57:36.34 Forearm.seek :retract @16:57:37.38 SE.stop @16:57:37.43 SC.reconfigure :tight @16:57:37.50 SC.seek 3 @16:57:40.93 SC.configure :limp @16:57:41.10 Elbow.seek 3 @16:57:41.67 Forearm.reconfigure :tight @16:57:41.74 Forearm.seek 3 + 450 counts @16:57:42.84 SE.reconfigure :place @16:57:42.92 SE.seek :beamBreak @16:57:44.59 SE.configure :default @16:57:44.71 SE.seek :down @16:57:45.67 SE.stop @16:57:45.84 Forearm.reconfigure :loose @16:57:45.92 Forearm.seek :retract @16:57:46.88 SC.reconfigure :tight @16:57:46.95 SC.seek 4 @16:57:50.52 SC.configure :limp @16:57:50.70 Elbow.seek 4 @16:57:51.16 Forearm.reconfigure :tight @16:57:51.24 Forearm.seek 4 + 450 counts @16:57:52.28 SE.reconfigure :place @16:57:52.36 SE.seek :beamBreak @16:57:53.52 SE.configure :default @16:57:53.65 SE.seek :down @16:57:54.10 SE.stop @16:57:54.78 Forearm.reconfigure :loose @16:57:54.85 Forearm.seek :retract @16:57:56.22 SC.reconfigure :tight @16:57:56.37 SC.seek 5 @16:58:00.00 SC.configure :limp @16:58:00.19 Elbow.seek 5 @16:58:00.49 Forearm.reconfigure :tight @16:58:00.56 Forearm.seek 5 + 450 counts @16:58:01.62 SE.reconfigure :place @16:58:01.70 SE.seek :beamBreak @16:58:02.85 SE.configure :default @16:58:02.98 SE.seek :down @16:58:03.41 SE.stop @16:58:04.11 Forearm.reconfigure :loose @16:58:04.19 Forearm.seek :retract @16:58:05.12 SC.reconfigure :tight @16:58:05.19 SC.seek 6 @16:58:08.72 SC.configure :limp @16:58:08.90 Elbow.seek 6 @16:58:09.12 Forearm.reconfigure :tight @16:58:09.19 Forearm.seek 6 + 450 counts @16:58:10.28 SE.reconfigure :place @16:58:10.36 SE.seek :beamBreak @16:58:11.56 SE.configure :default @16:58:11.69 SE.seek :down @16:58:12.13 SE.stop @16:58:12.83 Forearm.reconfigure :loose @16:58:12.90 Forearm.seek :retract @16:58:13.86 SC.reconfigure :tight @16:58:13.94 SC.seek 7 @16:58:17.47 SC.configure :limp @16:58:17.65 Elbow.seek 7 @16:58:18.18 Forearm.reconfigure :tight @16:58:18.26 Forearm.seek 7 + 450 counts @16:58:19.38 SE.reconfigure :place @16:58:19.46 SE.seek :beamBreak @16:58:20.77 SE.configure :default @16:58:20.90 SE.seek :down @16:58:21.55 SE.stop @16:58:22.04 Forearm.reconfigure :loose @16:58:22.11 Forearm.seek :retract {1 => 33, 2 => 7, 3 => 26, 4 => 33, 5 => 33, 6 => 33, 7 => 31} @16:58:57.89 -> abort! 'mission complete' @16:58:57.90 CmdServer::Client::Error in dave -- Aborting Mission: mission complete @16:58:57.95 BadNews.email "mission complete",Subject: "DA_WCR_101823 ABORTED!" # @16:59:03.65 upsync @16:59:03.74 Session Ended + ESPwhich DA_WCR_102423.rb + fn=DA_WCR_102423.rb + IFS=: + set /home/esp/esp2/mission . + fullPath=/home/esp/esp2/mission/DA_WCR_102423.rb + [ -f /home/esp/esp2/mission/DA_WCR_102423.rb ] + realpath /home/esp/esp2/mission/DA_WCR_102423.rb + return 0 + scriptPath=/home/esp/esp2local/mission/DA_WCR_102423.rb + exec ruby /home/esp/esp2/lib/esp.rb /home/esp/esp2local/mission/DA_WCR_102423.rb @13:48:42.17UTC24-Oct-23
ESP2 v3.42 I2C Gateway v3.20 for core bus @13:48:44.88 Configuration for short puck jake 1/11/21 by brent@mbari.org /home/esp/esp2/type/shallow/jake/configure.rb @13:48:48.57 Polling Can every 10:00 @13:48:48.64 Cleanly exited at 13:46:15UTC24-Oct-23 @13:48:48.68 Loading /home/esp/esp2local/mission/DA_WCR_102423.rb mission @13:48:48.74 GoodNews.email "Starting DA_WCR_102423 w/pucks in tube 3\n{1 => 33,\n 2 => 7,\n 3 => 26,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@13:48:48, 28% flow, 17.3C, 20% humidity, 14.6psia, 13.749V, 0.236A, 212.531Ah, 3.25W",Subject: "DA_WCR_102423 begun" @13:48:48.78
Preparing to sleep until + 00:00:05 @13:48:48.81 Asleep til + 00:00:05 @13:48:48.83 Continuing at 13:48 @13:48:49.12 Gate.power :core,:OFF @13:48:49.13 Gate.power :raw,:OFF @13:48:49.14 Shutdown @13:48:51.65 GoodNews.email "Then, in tube 3: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_102423.rb:14\n{1 => 33,\n 2 => 7,\n 3 => 26,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@13:48:48, 28% flow, 17.3C, 20% humidity, 14.6psia, 13.749V, 0.242A, 212.531Ah, 3.33W",Subject: "Asleep until 13:48" @13:48:53.04 upsync @13:48:53.72
Powering core bus @13:48:55.57 upsync @13:48:59.06
Gate.power :raw,:ON @13:48:59.67 CS.configure :default @13:48:59.72 PS.configure :default @13:48:59.76 SS.configure :default @13:48:59.81 CC.configure :default @13:48:59.86 PC.configure :default @13:48:59.92 Forearm.configure :loose @13:48:59.97 Elbow.configure :loose @13:49:00.04 SC.configure :loose @13:49:00.09 SE.configure :default @13:49:00.13 CH.configure :default @13:49:00.17 PH.configure :default @13:49:00.21 All dwarf controllers running firmware version 3.85 @13:49:00.32 Valve.reset @13:49:00.58 Intake.select :close @13:49:00.62 Exhaust.select :close @13:49:00.63 CTV.dial :air @13:49:00.72 PSV.dial :air @13:49:00.82
Forearm.home.seek :retract @13:49:01.60 Hand.close @13:49:01.63
Elbow.reconfigure :loose @13:49:01.72 Elbow.jog 1500 @13:49:01.81 PS.home.seek 0ml @13:49:02.00 CSV.dial :waste1 @13:49:02.24
Elbow.reconfigure :loose @13:49:02.31 Elbow.home.seek :clear @13:49:02.72 SSV.dial :spit,avoiding: :exhaust @13:49:03.60 CS.reconfigure :fast @13:49:03.64 SS.home.seek 0ml @13:49:03.73 CS.home.seek 0ml @13:49:06.77
SE.home.seek :down @13:49:07.36 SC.reconfigure :tight @13:49:07.41 SC.home.seek :initial @13:49:10.19 PS.stop @13:49:35.75
SC.configure :limp @13:49:35.80 Forearm.seek :retract @13:49:35.87 Elbow.seek :hammer @13:49:36.92 Forearm.reconfigure :tight @13:49:36.97 Forearm.seek retracted @13:49:37.33 PC.home.seek :open @13:49:37.34
CC.home.seek :open @13:49:41.13 Phase [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] @13:49:41.15 Notice.email "At 13:49:41 began in tube 3: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_102423.rb:14\n{1 => 33,\n 2 => 7,\n 3 => 26,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@13:49:41, 28% flow, 17.2C, 20% humidity, 14.6psia, 13.666V, 0.278A, 212.537Ah, 3.80W",Subject: "Started DA_WCR_102423 phase initialPurge; da [1000, 1000] #da and wsr are run simultanelously" @13:49:41.17
Starting initialPurge @13:49:41.19 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @13:49:41.26 Hand.open @13:49:42.04 Elbow.reconfigure :tight @13:49:42.11 Elbow.seek FlushPuck::Garage @13:49:44.12 Elbow.reconfigure :loose @13:49:44.24 Forearm.reconfigure :loose @13:49:44.29 Forearm.seek :garage @13:49:45.23 Hand.close @13:49:45.28 upsync @13:49:45.92
Forearm.seek :retract @13:49:46.89 Elbow.reconfigure :tight @13:49:46.93 Elbow.seek CC @13:49:49.90 Elbow.reconfigure :loose @13:49:50.00 Forearm.reconfigure :tight @13:49:50.05 Forearm.seek CC @13:49:51.32 CC.seek :closed @13:49:55.90 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.28 @13:49:55.97 Hand.open @13:49:56.61 Forearm.reconfigure :loose @13:49:56.65 Forearm.seek :retract @13:49:57.65 Flushing the Collection Valve::Manifold @13:49:57.77 CSV.dial CSR @13:49:59.07 Pulling 5ml of CSR.flush CSR.select :flush @13:49:59.19 CS.reconfigure :default @13:49:59.65 CS.seek 5ml @13:50:13.61 delay 5 seconds @13:50:18.63 CSR.select :air @13:50:18.80 CS.reconfigure :fast @13:50:18.85 CS.seek 7.499ml @13:50:25.83 CSV.dial :waste @13:50:27.12 CS.reconfigure :default @13:50:27.16 CS.seek 0ml @13:50:47.18 Purging external Intake @13:50:47.30 CSV.dial :flush @13:50:47.76 CS.reconfigure :fast @13:50:47.80 CS.seek 10ml @13:51:12.36 delay 5 seconds @13:51:17.38 CSV.dial CBV @13:51:17.43
CBV.dial CSV @13:51:18.19 CTV.dial :intake @13:51:18.99 Intake.select :open @13:51:19.10 CS.seek 0ml @13:51:42.70 Intake.select :close @13:51:42.80 CTV.dial :air @13:51:43.60 Flushing upper manifold @13:51:43.72 CSV.dial :flush @13:51:44.46 CS.seek 5ml @13:51:57.33 delay 5 seconds @13:52:02.35 CSV.dial :air @13:52:02.86 CS.seek 7.001ml @13:52:08.52 CSV.dial CBV @13:52:08.55
CBV.dial CSV @13:52:09.59 CTV.dial :air @13:52:09.68 CS.seek 0ml @13:52:26.45 delay 7 seconds @13:52:33.48 CS.seek 3ml @13:52:41.51 CSV.dial :waste @13:52:41.97 CS.seek 0ml @13:52:49.72 CSV.dial :flush @13:52:50.29 CS.seek 3ml @13:52:58.33 delay 5 seconds @13:53:03.35 CSV.dial :air @13:53:03.88 CS.seek 5.001ml @13:53:09.42 CSV.dial :air @13:53:09.61 CS.seek 0ml @13:53:21.70 delay 7 seconds @13:53:28.73 CS.seek 3ml @13:53:36.70 CSV.dial :waste @13:53:37.43 CS.seek 0ml @13:53:45.14 Garaging FlushPuck in Collection Clamp @13:53:45.21 Elbow.reconfigure :tight @13:53:45.26 Elbow.seek CC @13:53:45.51 Elbow.reconfigure :loose @13:53:45.61 Forearm.seek CC @13:53:46.58 Hand.close @13:53:47.16 CC.seek :open @13:53:52.20 Forearm.seek :retract @13:53:53.19 Elbow.reconfigure :tight @13:53:53.24 Elbow.seek :garage @13:53:56.23 Elbow.reconfigure :loose @13:53:56.33 Forearm.reconfigure :tight @13:53:56.38 Forearm.seek :garage @13:53:57.35 Hand.open @13:53:57.97 Forearm.reconfigure :loose @13:53:58.01 Forearm.seek :retract @13:53:58.99 da sampling at most 1000ml, wcr at most 1000ml @13:53:59.03 Loading sh1 puck from tube 3 into Collection Clamp @13:53:59.04 DAfans.email "Started da in tube 3 at 13:53:59UTC24-Oct-23\n{1 => 33,\n 2 => 7,\n 3 => 26,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@13:49:41, 28% flow, 17.2C, 20% humidity, 14.6psia, 13.666V, 0.278A, 212.537Ah, 3.80W",Subject: "da sampling at most 1000ml, wcr at most 1000ml" @13:53:59.31
SC.reconfigure :tight @13:53:59.37 SC.seek 3 @13:54:02.96 SC.configure :limp @13:54:03.10 upsync @13:54:03.23
Elbow.reconfigure :tight @13:54:03.28 Elbow.seek 3 @13:54:05.20 Forearm.reconfigure :tight @13:54:05.25 Forearm.seek 3 + 450 counts @13:54:06.38 SE.reconfigure :pick @13:54:06.44 SE.seek :beamBreak @13:54:07.96 SE.configure :default @13:54:08.13 Elbow.seek 3 @13:54:08.32 Forearm.seek 3 + 300 counts @13:54:08.56 Forearm.reconfigure :loose @13:54:08.61 Hand.close @13:54:09.30 SE.seek :down @13:54:09.89
Forearm.seek :retract @13:54:10.40 SE.stop @13:54:10.91
Elbow.seek CC @13:54:12.54 Elbow.reconfigure :loose @13:54:12.65 Forearm.reconfigure :tight @13:54:12.69 Forearm.seek CC @13:54:14.02 CC.seek :closed @13:54:18.54 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.28 @13:54:18.56 Hand.open @13:54:19.22 Forearm.reconfigure :loose @13:54:19.26 Forearm.seek :retract @13:54:20.22 collectSample 1000ml w/bubble point of 24psi @13:54:20.23 Priming 7ml @13:54:20.25 Intake.select :close @13:54:20.26 Exhaust.select :close @13:54:20.27 SS.hold @13:54:20.48 Calibrating pressure and temperature sensors @13:54:20.51 CTV.dial :air @13:54:20.59 SSV.dial :spit,avoiding: :exhaust @13:54:20.67 SS.reconfigure :fast @13:54:20.72 SS.seek 5ml @13:54:23.68 SSV.dial CBV @13:54:23.71
CBV.dial SSV @13:54:25.06 delay 3.8 seconds @13:54:28.98 Sensor limits: Intake max 109.7psia; Outlet max 102.4psia @13:54:28.99 SSV.dial :spit @13:54:30.31 SS.seek 0ml @13:54:32.96 SSV.dial CBV @13:54:34.29 CTV.dial :intake @13:54:35.09 SSV.dial CBV @13:54:35.12
CBV.dial SSV @13:54:35.34 Allowing 40s for 7.0ml of remaining 7.0ml @13:54:35.36 Intake.select :open @13:54:37.52 Intake @14.8psia, vacuum from 9.3 to 12.3psi @13:54:37.54 SS.reconfigure :inhaling 2.20ml/s @13:54:37.58 SS.seek 7ml @13:54:44.27 Stroke completed -- waiting for pressure to equalize ... @13:54:44.28 SS.reconfigure :exhale @13:54:44.33 SS.hold @13:55:15.37 SS.reconfigure :default @13:55:15.41 SS.hold @13:55:15.48 Intake.select :close @13:55:15.63 ShallowSampler::Clogged in MAIN -- Filtering too Slowly after 7.0ml @13:55:15.64 SSV.dial :exhaust @13:55:16.40 Exhaust.select :open @13:55:16.51 SS.reconfigure :exhale @13:55:16.55 SS.seek 0ml @13:55:20.91 Exhaust.select :close @13:55:21.03 Sampling 1000.0ml @13:55:21.04 Intake.select :close @13:55:21.05 Exhaust.select :close @13:55:21.06 SS.reconfigure :default @13:55:21.10 SS.hold @13:55:21.30 CTV.dial :intake @13:55:21.47 SSV.dial CBV @13:55:21.51
CBV.dial SSV @13:55:22.26 Allowing 130s for 25.0ml of remaining 1000.0ml @13:55:22.27 Intake.select :open @13:55:24.86 Intake @14.8psia, vacuum from 9.3 to 12.3psi @13:55:24.88 SS.reconfigure :inhaling 2.20ml/s @13:55:24.92 SS.seek 25ml @13:55:29.70 Vacuum too high -- waiting up to 40 seconds @13:55:29.72 SS.reconfigure :exhale @13:55:29.77 SS.hold @13:56:10.39 SS.reconfigure :default @13:56:10.44 SS.hold @13:56:10.50 Intake.select :close @13:56:10.65 ShallowSampler::Clogged in MAIN -- Intake @14.8psia, & Outlet @2.3psia -- no progress after 4.8ml @13:56:10.67 SSV.dial :exhaust @13:56:11.43 Exhaust.select :open @13:56:11.54 SS.reconfigure :exhale @13:56:11.59 SS.seek 0ml @13:56:15.13 Exhaust.select :close @13:56:15.24 Sampled 4.8ml @13:56:15.26 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @13:56:15.28 Intake.select :close @13:56:15.34 CSV.dial :waste @13:56:15.48 SSV.dial CBV,avoiding: :exhaust @13:56:15.51
CBV.dial SSV @13:56:15.64 CS.seek 0ml @13:56:15.71 CSV.dial :air @13:56:16.44 CS.seek 10ml @13:56:17.40
CTV.dial :air @13:56:18.19 delay 15 seconds @13:56:33.21 SSV.dial :spit,avoiding: :exhaust @13:56:34.53 CTV.dial CSV,avoiding: :intake @13:56:34.57
SS.seek 0ml @13:56:34.68 SSV.dial CBV,avoiding: :exhaust @13:56:41.11 SSV.dial between CBV and plug @13:56:41.59 CBV.dial between SSV and waste2 @13:56:41.98 CTV.dial CSV,avoiding: :air @13:56:42.01
CSV.dial CTV @13:56:42.49 CS.reconfigure :pressurize @13:56:42.53 CS.seek 0ml @13:57:02.61 BubblePoint: Intake @24.2psia, Outlet @-10.7psia @13:57:02.62 CBV.dial SSV @13:57:02.95 SS.reconfigure :default @13:57:03.00 SS.seek 10ml @13:57:08.63 delay 15 seconds @13:57:23.76 Evacuated: Intake @23.9psia, Outlet @-10.4psia @13:57:23.78 SSV.dial :spit,avoiding: :exhaust @13:57:24.82 SS.reconfigure :exhale @13:57:24.88 SS.seek 0ml @13:57:30.16 SS.reconfigure :fast @13:57:30.20 SS.seek 5ml @13:57:33.03 SSV.dial CBV,avoiding: :exhaust @13:57:33.05
CBV.dial SSV @13:57:34.40 CTV.dial :air,avoiding: :intake @13:57:35.75 CSV.dial CTV @13:57:35.78
SS.seek 10ml @13:57:36.91 CSV.dial :air @13:57:37.36 CS.reconfigure :fast @13:57:37.41 CS.seek 0ml @13:57:38.43
SSV.dial :spit,avoiding: :exhaust @13:57:40.11 SS.reconfigure :exhale @13:57:40.23 SS.seek 0ml @13:57:43.67 CS.stop @13:57:45.25
SS.reconfigure :fast @13:57:45.30 SS.seek 5ml @13:57:48.07 SSV.dial CBV,avoiding: :exhaust @13:57:48.10
CBV.dial SSV @13:57:50.42 SSV.dial :spit,avoiding: :exhaust @13:57:51.75 SS.reconfigure :default @13:57:51.79 SS.seek 0ml @13:57:55.05 SS.stop @13:57:55.09 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @13:57:55.10 Intake.select :close @13:57:55.17 CSV.dial :waste @13:57:55.29 SSV.dial CBV,avoiding: :exhaust @13:57:55.32
CBV.dial SSV @13:57:55.96 CS.seek 0ml @13:57:56.02 CSV.dial :air @13:57:56.65
CTV.dial :air @13:57:56.74 delay 15 seconds @13:57:56.78 CS.seek 10ml @13:58:11.76
SSV.dial :spit,avoiding: :exhaust @13:58:13.07 CTV.dial CSV,avoiding: :intake @13:58:13.11
SS.reconfigure :exhale @13:58:13.20 SS.seek 0ml @13:58:13.62 SSV.dial CBV,avoiding: :exhaust @13:58:21.26 SSV.dial between CBV and plug @13:58:21.74 CBV.dial between SSV and waste2 @13:58:22.13 CSV.dial between air and CTV @13:58:22.56 CS.seek 7.311ml @13:58:29.55 CTV.dial CSV,avoiding: :air @13:58:29.58
CSV.dial CTV @13:58:29.94 CS.reconfigure :pressurize @13:58:29.99 CS.seek 0ml @13:58:45.21 BubblePoint: Intake @24.3psia, Outlet @-0.8psia @13:58:45.22 CBV.dial SSV @13:58:45.55 SS.reconfigure :default @13:58:45.59 SS.seek 10ml @13:58:51.22 delay 15 seconds @13:59:06.36 Evacuated: Intake @23.9psia, Outlet @-0.1psia @13:59:06.37 SSV.dial :spit,avoiding: :exhaust @13:59:07.43 SS.reconfigure :exhale @13:59:07.48 SS.seek 0ml @13:59:12.54 SS.reconfigure :fast @13:59:12.58 SS.seek 5ml @13:59:15.65 SSV.dial CBV,avoiding: :exhaust @13:59:15.69
CBV.dial SSV @13:59:16.99 CTV.dial :air,avoiding: :intake @13:59:18.36 CSV.dial CTV @13:59:18.39
SS.seek 10ml @13:59:19.61 CSV.dial :air @13:59:20.04 CS.reconfigure :fast @13:59:20.09 CS.seek 0ml @13:59:21.37
SSV.dial :spit,avoiding: :exhaust @13:59:22.69 SS.reconfigure :exhale @13:59:22.74 SS.seek 0ml @13:59:24.98 CS.stop @13:59:27.78
SS.reconfigure :fast @13:59:27.84 SS.seek 5ml @13:59:30.67 SSV.dial CBV,avoiding: :exhaust @13:59:30.69
CBV.dial SSV @13:59:33.01 SSV.dial :spit,avoiding: :exhaust @13:59:34.34 SS.reconfigure :default @13:59:34.38 SS.seek 0ml @13:59:37.66 SS.stop @13:59:37.70 Lysing: Adding 2.0ml AqMeOH @13:59:37.71 CBV.dial between CSV and waste1,avoiding: :DBV @13:59:39.21 CTV.dial CSV @13:59:40.54 CSV.dial CSR @13:59:41.33 Pulling 1ml of CSR.AqMeOH @13:59:41.34 CSR.select :AqMeOH @13:59:41.45 CS.reconfigure :default @13:59:41.50 CS.seek 1ml @13:59:45.10 delay 5 seconds @13:59:50.12 CSR.select :air @13:59:50.29 CS.seek 6ml @14:00:03.88 delay 2 seconds @14:00:05.90 CSV.dial :waste2 @14:00:06.34 CS.reconfigure :fast @14:00:06.39 CS.seek 0ml @14:00:20.79 CSV.dial CSR @14:00:21.24 Pulling 2ml of CSR.AqMeOH @14:00:21.25 CSR.select :AqMeOH @14:00:21.37 CS.reconfigure :default @14:00:21.41 CS.seek 2ml @14:00:27.35 delay 5 seconds @14:00:32.37 CSR.select :air @14:00:32.54 CS.seek 5ml @14:00:41.01 delay 2 seconds @14:00:43.02 CSV.dial CTV @14:00:43.47 CS.seek 1ml @14:00:54.71 Before lysing Collection Puck Heater at 16.8C/50.8psia @14:00:54.73 Heating Puck.from 3, :sh1 in Collection Clamp to 70C for 10:00 @14:00:54.75 CH.seek 70C,8:00 @14:03:07.46 CH.hold 10:00,70C @14:03:07.65 Begun lysing Collection Puck Heater at 68C/49.3psia @14:13:07.66 Ended lysing Collection Puck Heater at 70C/43.5psia @14:13:07.68 CS.seek 0ml @14:13:11.22 CTV.dial between spare and air,avoiding: :intake @14:13:12.26 CSV.dial :air @14:13:12.91 CS.seek 5ml @14:13:26.49 CSV.dial CTV @14:13:26.93 delay 2 seconds @14:13:28.95 CSV.dial :air @14:13:29.40 delay 2 seconds @14:13:31.42 CSV.dial CTV @14:13:31.88 delay 2 seconds @14:13:33.89 CSV.dial :air @14:13:34.34 delay 2 seconds @14:13:36.36 CS.seek 0ml @14:13:50.02 Puck @95.1psia after final lysis pressurization @14:13:50.03 Recovering Lysate into Collection syringe @14:13:50.04 CSV.dial CBV @14:13:51.06 CBV.dial CSV @14:13:51.43 CS.seek 5ml @14:14:05.28 delay 5 seconds @14:14:10.29 CTV.dial :air @14:14:10.82 CS.seek 7ml @14:14:16.71 delay 5 seconds @14:14:21.73 CSV.dial :air2 @14:14:22.17 CS.seek 10ml @14:14:30.59 CSV.dial between air and CTV,avoiding: CBV @14:14:31.75 Lysing Complete @14:14:31.82 Storing sh1 puck in Collection Clamp to tube 2 @14:14:31.99 SC.reconfigure :tight @14:14:32.04 SC.seek 2 @14:14:35.54 SC.configure :limp @14:14:35.76 Elbow.reconfigure :tight @14:14:35.81 Elbow.seek 2 @14:14:37.56 Forearm.reconfigure :tight @14:14:37.60 Forearm.seek 2 + 450 counts @14:14:38.69 SE.reconfigure :pick @14:14:38.74 SE.seek :beamBreak @14:14:41.53 SE.configure :default @14:14:41.68 Elbow.reconfigure :limp @14:14:41.73 Forearm.reconfigure :loose @14:14:41.78 Forearm.seek 2 @14:14:42.08 Elbow.hold @14:14:42.14 Elbow.reconfigure :tight @14:14:42.24 SE.seek 6.14 Pucks below Top Plate @14:14:42.65 Forearm.force -20 @14:14:42.70 Forearm.seek 2 + 287 counts @14:14:43.38 Forearm.reconfigure :tight @14:14:43.50 Forearm.seek 2 + 450 counts @14:14:43.71 SE.seek 7.14 Pucks below Top Plate @14:14:44.22 Forearm.reconfigure :loose @14:14:44.27 Forearm.seek :retract @14:14:45.21 Elbow.seek CC @14:14:46.89 Elbow.reconfigure :loose @14:14:46.99 Forearm.seek CC @14:14:48.05 Hand.close @14:14:48.64 CC.seek :open @14:14:53.41 Forearm.seek :retract @14:14:54.39 Elbow.reconfigure :tight @14:14:54.44 Elbow.seek 2 @14:14:56.24 Elbow.reconfigure :limp @14:14:56.29 Forearm.seek 2 @14:14:57.28 Elbow.hold @14:14:57.35 Elbow.reconfigure :tight @14:14:57.39 Hand.open @14:14:58.13 Forearm.force -20 @14:14:58.18 Forearm.seek 2 + 320 counts @14:14:58.48 Forearm.reconfigure :tight @14:14:58.53 Forearm.seek 2 + 450 counts @14:14:58.83 SE.seek :down @14:14:59.97
Forearm.reconfigure :loose @14:15:00.01 Forearm.seek :retract @14:15:01.04 SE.stop @14:15:01.08
Tranferring DA @14:15:01.09 Beginning samplePassC @14:15:01.11 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:15:01.29 Elbow.seek FlushPuck::Garage @14:15:03.02 Elbow.reconfigure :loose @14:15:03.12 Forearm.seek :garage @14:15:04.55 Hand.close @14:15:05.27 Forearm.seek :retract @14:15:06.25 Elbow.reconfigure :tight @14:15:06.30 Elbow.seek CC @14:15:09.23 Elbow.reconfigure :loose @14:15:09.33 Forearm.reconfigure :tight @14:15:09.38 Forearm.seek CC @14:15:10.68 CC.seek :closed @14:15:15.21 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.40 @14:15:15.28 Hand.open @14:15:15.95 Forearm.reconfigure :loose @14:15:16.00 Forearm.seek :retract @14:15:16.95 PSV.dial PSR @14:15:18.34 Pulling 1.5ml of PSR.flush @14:15:18.35 PSR.select :flush @14:15:18.47 PS.seek 1.5ml @14:15:34.60 delay 5 seconds @14:15:39.62 PSV.dial :waste1 @14:15:40.10 PS.seek 0ml @14:15:56.22 PSV.dial PSR @14:15:56.69 PSR.select :air @14:15:56.81 PS.seek 2.5ml @14:16:23.16 PRV.dial CBV,avoiding: :ab2 @14:16:23.19
CBV.dial PRV @14:16:24.94 PSV.dial :mixloop @14:16:26.10 CTV.dial :air,avoiding: :intake @14:16:26.18 PTV.dial :PRVmixing @14:16:27.51 PS.seek 0ml @14:16:53.88 PSV.dial :air @14:16:54.35 PS.seek 2.5ml @14:17:21.16 PSV.dial :mixloop @14:17:21.63 PS.seek 0ml @14:17:47.94 PSV.dial :air @14:17:48.41 PS.seek 2.5ml @14:18:14.94 PSV.dial :mixloop @14:18:15.41 PS.seek 0ml @14:18:41.69 Garaging FlushPuck in Collection Clamp @14:18:41.76 Elbow.reconfigure :tight @14:18:41.81 Elbow.seek CC @14:18:42.10 Elbow.reconfigure :loose @14:18:42.20 Forearm.seek CC @14:18:43.25 Hand.close @14:18:43.89 CC.seek :open @14:18:48.51 Forearm.seek :retract @14:18:49.50 Elbow.reconfigure :tight @14:18:49.55 Elbow.seek :garage @14:18:52.47 Elbow.reconfigure :loose @14:18:52.57 Forearm.reconfigure :tight @14:18:52.62 Forearm.seek :garage @14:18:53.72 Hand.open @14:18:54.45 Forearm.reconfigure :loose @14:18:54.49 Forearm.seek :retract @14:18:55.46 PSV.dial :mixloop @14:18:55.55 PTV.dial :PRVmixing @14:18:55.63 PRV.dial CBV @14:18:55.66
CBV.dial PRV @14:18:55.80 CTV.dial :air,avoiding: :intake @14:18:56.65 Loading lyfil puck from tube 3 into Collection Clamp @14:18:56.89 SC.reconfigure :tight @14:18:56.94 SC.seek 3 @14:19:00.52 SC.configure :limp @14:19:00.74 Elbow.reconfigure :tight @14:19:00.78 Elbow.seek 3 @14:19:03.17 Forearm.reconfigure :tight @14:19:03.22 Forearm.seek 3 + 450 counts @14:19:04.26 SE.reconfigure :pick @14:19:04.32 SE.seek :beamBreak @14:19:05.83 SE.configure :default @14:19:05.99 Elbow.seek 3 @14:19:06.19 Forearm.seek 3 + 300 counts @14:19:06.48 Forearm.reconfigure :loose @14:19:06.53 Hand.close @14:19:07.15 SE.seek :down @14:19:07.35
Forearm.seek :retract @14:19:08.28 Elbow.seek CC @14:19:08.35 SE.stop @14:19:09.88
Elbow.reconfigure :loose @14:19:09.98 Forearm.reconfigure :tight @14:19:10.03 Forearm.seek CC @14:19:11.28 CC.seek :closed @14:19:15.81 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.16 @14:19:15.82 Hand.open @14:19:16.45 Forearm.reconfigure :loose @14:19:16.49 Forearm.seek :retract @14:19:17.45 CTV.dial CSV,avoiding: :intake @14:19:18.82 CSV.dial CTV @14:19:19.61 PS.seek 2.5ml @14:19:46.06 CS.seek 4.5ml @14:20:00.96 delay 8 seconds @14:20:08.98 PSV.dial :air @14:20:09.45 delay 2 seconds @14:20:11.47 PSV.dial :waste2 @14:20:12.21 PS.seek 0ml @14:20:38.65 CBV.dial between PRV and waste1 @14:20:38.99 CSV.dial :air @14:20:39.45 CS.seek 10ml @14:20:54.30 CSV.dial CTV @14:20:54.76 CS.seek 0ml @14:21:21.42 CBV.dial :waste1 @14:21:21.74 delay 5 seconds @14:21:26.76 CTV.dial :air,avoiding: :intake @14:21:28.19 Storing lyfil puck in Collection Clamp to tube 2 @14:21:28.36 SC.reconfigure :tight @14:21:28.41 SC.seek 2 @14:21:31.95 SC.configure :limp @14:21:32.16 Elbow.reconfigure :tight @14:21:32.21 Elbow.seek 2 @14:21:34.02 Forearm.reconfigure :tight @14:21:34.07 Forearm.seek 2 + 450 counts @14:21:35.09 SE.reconfigure :pick @14:21:35.14 SE.seek :beamBreak @14:21:37.75 SE.configure :default @14:21:37.91 Elbow.reconfigure :limp @14:21:37.96 Forearm.reconfigure :loose @14:21:38.01 Forearm.seek 2 @14:21:38.26 Elbow.hold @14:21:38.32 Elbow.reconfigure :tight @14:21:38.42 SE.seek 7.15 Pucks below Top Plate @14:21:39.33 Forearm.force -20 @14:21:39.37 Forearm.seek 2 + 304 counts @14:21:39.74 Forearm.reconfigure :tight @14:21:39.79 Forearm.seek 2 + 450 counts @14:21:40.02 SE.seek 8.15 Pucks below Top Plate @14:21:40.53 Forearm.reconfigure :loose @14:21:40.58 Forearm.seek :retract @14:21:41.53 Elbow.seek CC @14:21:43.24 Elbow.reconfigure :loose @14:21:43.34 Forearm.seek CC @14:21:44.32 Hand.close @14:21:44.89 CC.seek :open @14:21:49.63 Forearm.seek :retract @14:21:50.59 Elbow.reconfigure :tight @14:21:50.64 Elbow.seek 2 @14:21:52.39 Elbow.reconfigure :limp @14:21:52.44 Forearm.seek 2 @14:21:53.42 Elbow.hold @14:21:53.48 Elbow.reconfigure :tight @14:21:53.53 Hand.open @14:21:54.26 Forearm.force -20 @14:21:54.29 Forearm.seek 2 + 302 counts @14:21:54.67 Forearm.reconfigure :tight @14:21:54.72 Forearm.seek 2 + 450 counts @14:21:54.98 SE.seek :down @14:21:56.56
Forearm.reconfigure :loose @14:21:56.61 Forearm.seek :retract @14:21:57.14 SE.stop @14:21:57.57
PSV.dial :air @14:21:58.33 PS.seek 2.5ml @14:22:24.69 delay 1 second @14:22:25.70 PSV.dial :mixloop @14:22:26.15 delay 1 second @14:22:27.17 PSV.dial :air @14:22:27.64 delay 1 second @14:22:28.65 PSV.dial :mixloop @14:22:29.12 delay 1 second @14:22:30.14 PSV.dial :air @14:22:30.61 delay 1 second @14:22:31.63 PS.seek 0ml @14:22:58.00 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @14:22:58.18 Elbow.seek FlushPuck::Garage @14:23:00.03 Elbow.reconfigure :loose @14:23:00.13 Forearm.seek :garage @14:23:01.12 Hand.close @14:23:01.88 Forearm.seek :retract @14:23:02.86 Elbow.reconfigure :tight @14:23:02.90 Elbow.seek PC @14:23:04.39 Elbow.reconfigure :loose @14:23:04.49 Forearm.reconfigure :tight @14:23:04.54 Forearm.seek PC @14:23:05.83 PC.seek :closed @14:23:10.50 Processing Clamp onSpring/offSpring for 16/26 samples, on/off torque 1.61 @14:23:10.56 Hand.open @14:23:11.70 Forearm.reconfigure :loose @14:23:11.75 Forearm.seek :retract @14:23:12.70 PRV.dial :air @14:23:13.15 PSV.dial :mixloop @14:23:13.68 PS.seek 0.3ml @14:23:17.59 PSV.dial PSR @14:23:18.65 Pulling 0.4ml of PSR.air @14:23:18.66 PSR.select :air @14:23:18.68 PS.seek 0.7ml @14:23:23.56 delay 3 seconds @14:23:26.57 PSR.select :air @14:23:26.60 PS.seek 2.5ml @14:23:45.74 delay 3 seconds @14:23:48.76 PSV.dial :mixloop @14:23:49.82 PTV.dial :mixing @14:23:50.59 PBV.dial :waste2 @14:23:51.56 PS.seek 0ml @14:24:17.93 Garaging FlushPuck in Processing Clamp @14:24:18.01 Elbow.reconfigure :tight @14:24:18.05 Elbow.seek PC @14:24:18.17 Elbow.reconfigure :loose @14:24:18.28 Forearm.seek PC @14:24:19.24 Hand.close @14:24:19.89 PC.seek :open @14:24:24.61 Forearm.seek :retract @14:24:25.59 Elbow.reconfigure :tight @14:24:25.64 Elbow.seek :garage @14:24:26.94 Elbow.reconfigure :loose @14:24:27.04 Forearm.reconfigure :tight @14:24:27.09 Forearm.seek :garage @14:24:28.16 Hand.open @14:24:28.82 Forearm.reconfigure :loose @14:24:28.86 Forearm.seek :retract @14:24:29.81 Loading da puck from tube 3 into Processing Clamp @14:24:30.05 SC.reconfigure :tight @14:24:30.10 SC.seek 3 @14:24:33.69 SC.configure :limp @14:24:34.35 Elbow.reconfigure :tight @14:24:34.39 Elbow.seek 3 @14:24:36.27 Forearm.reconfigure :tight @14:24:36.31 Forearm.seek 3 + 450 counts @14:24:37.35 SE.reconfigure :pick @14:24:37.41 SE.seek :beamBreak @14:24:39.01 SE.configure :default @14:24:39.16 Elbow.seek 3 @14:24:39.35 Forearm.seek 3 + 300 counts @14:24:39.62 Forearm.reconfigure :loose @14:24:39.66 Hand.close @14:24:40.27 SE.seek :down @14:24:40.47
Forearm.seek :retract @14:24:41.44 Elbow.seek PC @14:24:41.51 SE.stop @14:24:43.98
Elbow.reconfigure :loose @14:24:44.08 Forearm.reconfigure :tight @14:24:44.13 Forearm.seek PC @14:24:45.43 PC.seek :closed @14:24:50.07 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.62 @14:24:50.08 Hand.open @14:24:50.69 Forearm.reconfigure :loose @14:24:50.74 Forearm.seek :retract @14:24:51.76 Heating Puck.from 3, :da in Processing Clamp to 28C for 2:00:00 @14:24:51.77 PH.seek 28C,5:00 @14:25:34.53 PH.hold 2:00:00,28C @14:25:34.57 delay 1:30 @14:27:04.62 PSR.select :air @14:27:04.64 PSV.dial PSR @14:27:05.68 Pulling 0.5ml of PSR.wash @14:27:05.69 PSR.select :wash @14:27:05.81 PS.seek 0.5ml @14:27:11.71 delay 5 seconds @14:27:16.73 PSR.select :air @14:27:16.86 PS.seek 2ml @14:27:32.96 PTV.dial :puck @14:27:33.71 PSV.dial PTV @14:27:34.47 PBV.dial :waste1 @14:27:35.86 PS.seek 1.5ml @14:27:41.87 delay 3 seconds @14:27:44.89 PBV.dial between PSV and waste1 @14:27:45.25 PS.seek 0ml @14:28:01.40 delay 5:00 @14:33:01.41 recoverPSRreagent08 @14:33:01.42 PTV.dial :puck @14:33:01.50 PBV.dial :waste1 @14:33:01.88 delay 30 seconds @14:33:31.89 PBV.dial between PSV and waste1 @14:33:32.27 PSR.select :air @14:33:32.28 PSV.dial PSR @14:33:33.16 PS.reconfigure :fast @14:33:33.21 PS.seek 2.5ml @14:33:57.11 PSV.dial PTV @14:33:57.97 delay 5 seconds @14:34:02.99 PSV.dial PSR @14:34:03.75 delay 5 seconds @14:34:08.77 PSV.dial PTV @14:34:09.53 delay 5 seconds @14:34:14.55 PSV.dial :air @14:34:15.30 PS.seek 0ml @14:34:38.77 PTV.dial :PRVmixing,via: PRV @14:34:40.09 PSV.dial :mixloop @14:34:40.56 PRV.dial :ab1 @14:34:41.32 PS.reconfigure :slow @14:34:41.36 PS.seek 0.15ml @14:34:44.89 delay 2 seconds @14:34:46.91 CBV.dial PRV @14:34:46.94
PRV.dial CBV @14:34:47.50 PS.seek 0.35ml @14:34:52.11 delay 2 seconds @14:34:54.12 CBV.dial between PRV and PSV @14:34:54.44 PRV.dial :air @14:34:54.90 PS.seek 0.9ml @14:35:06.57 PRV.dial :waste2 @14:35:07.33 PS.seek 0.5ml @14:35:15.98 addPMLReagent08 @14:35:15.99 PSV.dial :air @14:35:16.46 PS.reconfigure :default @14:35:16.50 PS.seek 2ml @14:35:32.85 PSV.dial :PML @14:35:33.33 PTV.dial :mixing @14:35:34.08 PBV.dial :waste1 @14:35:34.51 PS.seek 1.85ml @14:35:37.20 PBV.dial between PSV and waste1 @14:35:37.58 PS.seek 0ml @14:35:57.26 delay 2 seconds @14:35:59.28 Beginning samplePassC @14:35:59.29 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:35:59.92 Elbow.reconfigure :tight @14:35:59.96 Elbow.seek FlushPuck::Garage @14:36:01.32 Elbow.reconfigure :loose @14:36:01.42 Forearm.seek :garage @14:36:02.44 Hand.close @14:36:03.12 Forearm.seek :retract @14:36:04.11 Elbow.reconfigure :tight @14:36:04.16 Elbow.seek CC @14:36:07.02 Elbow.reconfigure :loose @14:36:07.12 Forearm.reconfigure :tight @14:36:07.17 Forearm.seek CC @14:36:08.53 CC.seek :closed @14:36:13.07 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.36 @14:36:13.14 Hand.open @14:36:13.81 Forearm.reconfigure :loose @14:36:13.86 Forearm.seek :retract @14:36:14.84 PSV.dial PSR @14:36:15.90 Pulling 1.5ml of PSR.flush @14:36:15.91 PSR.select :flush @14:36:16.04 PS.seek 1.5ml @14:36:32.23 delay 5 seconds @14:36:37.25 PSV.dial :waste1 @14:36:37.70 PS.seek 0ml @14:36:53.85 PSV.dial PSR @14:36:54.33 PSR.select :air @14:36:54.44 PS.seek 2.5ml @14:37:20.73 PRV.dial CBV,avoiding: :ab2 @14:37:20.76
CBV.dial PRV @14:37:22.82 PSV.dial :mixloop @14:37:23.87 CTV.dial :air,avoiding: :intake @14:37:24.11 PTV.dial :PRVmixing @14:37:24.88 PS.seek 0ml @14:37:51.16 PSV.dial :air @14:37:51.64 PS.seek 2.5ml @14:38:18.04 PSV.dial :mixloop @14:38:18.52 PS.seek 0ml @14:38:44.88 PSV.dial :air @14:38:45.36 PS.seek 2.5ml @14:39:11.66 PSV.dial :mixloop @14:39:12.14 PS.seek 0ml @14:39:38.42 Garaging FlushPuck in Collection Clamp @14:39:38.49 Elbow.reconfigure :tight @14:39:38.54 Elbow.seek CC @14:39:38.78 Elbow.reconfigure :loose @14:39:38.88 Forearm.seek CC @14:39:39.92 Hand.close @14:39:40.50 CC.seek :open @14:39:45.15 Forearm.seek :retract @14:39:46.14 Elbow.reconfigure :tight @14:39:46.19 Elbow.seek :garage @14:39:49.11 Elbow.reconfigure :loose @14:39:49.21 Forearm.reconfigure :tight @14:39:49.26 Forearm.seek :garage @14:39:50.28 Hand.open @14:39:50.93 Forearm.reconfigure :loose @14:39:50.98 Forearm.seek :retract @14:39:51.97 delayUntil "14:43:59.27" @14:39:51.99 Whole Cell Archive sampling 1000ml @14:39:52.01 Loading wcr puck from tube 3 into Collection Clamp @14:39:52.93 Elbow.reconfigure :tight @14:39:52.99 Elbow.seek 3 @14:39:54.92 Forearm.reconfigure :tight @14:39:54.97 Forearm.seek 3 + 450 counts @14:39:56.01 SE.reconfigure :pick @14:39:56.07 SE.seek :beamBreak @14:39:57.70 SE.configure :default @14:39:58.63 Elbow.seek 3 @14:39:58.83 Forearm.seek 3 + 300 counts @14:39:59.07 Forearm.reconfigure :loose @14:39:59.12 Hand.close @14:39:59.76 SE.seek :down @14:39:59.98 Forearm.seek :retract @14:40:00.99 Elbow.seek CC @14:40:01.06 SE.stop @14:40:02.63 Elbow.reconfigure :loose @14:40:02.74 Forearm.reconfigure :tight @14:40:02.79 Forearm.seek CC @14:40:04.05 CC.seek :closed @14:40:08.95 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.26 @14:40:09.04 Hand.open @14:40:09.69 Forearm.reconfigure :loose @14:40:09.74 Forearm.seek :retract @14:40:10.73 collectSample 1000ml w/bubble point of 35psi @14:40:10.75 Priming 7ml @14:40:10.76 Intake.select :close @14:40:10.77 Exhaust.select :close @14:40:10.78 SS.hold @14:40:11.00 Calibrating pressure sensors @14:40:11.02 CTV.dial :air @14:40:11.10 SSV.dial :spit,avoiding: :exhaust @14:40:11.18 SS.reconfigure :fast @14:40:11.23 SS.seek 5ml @14:40:13.91 SSV.dial CBV @14:40:13.94 CBV.dial SSV @14:40:15.30 delay 3.8 seconds @14:40:19.23 Sensor limits: Intake max 111.1psia; Outlet max 102.6psia @14:40:19.24 SSV.dial :spit @14:40:20.59 SS.seek 0ml @14:40:23.72 SSV.dial CBV @14:40:25.07 CTV.dial :intake @14:40:25.86 SSV.dial CBV @14:40:25.89 CBV.dial SSV @14:40:26.03 Allowing 40s for 7.0ml of remaining 7.0ml @14:40:26.05 Intake.select :open @14:40:28.22 Intake @14.8psia, vacuum from 9.3 to 12.3psi @14:40:28.24 SS.reconfigure :inhaling 2.20ml/s @14:40:28.28 SS.seek 7ml @14:40:33.61 Stroke completed -- waiting for pressure to equalize ... @14:40:33.62 SS.reconfigure :exhale @14:40:33.67 SS.hold @14:40:46.37 Intake.select :close @14:40:46.53 SSV.dial :exhaust @14:40:47.30 Exhaust.select :open @14:40:47.41 SS.seek 0ml @14:40:51.56 Exhaust.select :close @14:40:51.68 Sampling 1000.0ml @14:40:51.69 Intake.select :close @14:40:51.70 Exhaust.select :close @14:40:51.71 SS.reconfigure :default @14:40:51.76 SS.hold @14:40:51.96 CTV.dial :intake @14:40:52.19 SSV.dial CBV @14:40:52.22 CBV.dial SSV @14:40:53.02 Allowing 130s for 25.0ml of remaining 1000.0ml @14:40:53.03 Intake.select :open @14:40:55.19 Intake @15.4psia, vacuum from 9.9 to 12.9psi @14:40:55.21 SS.reconfigure :inhaling 2.20ml/s @14:40:55.26 SS.seek 25ml @14:41:03.62 Vacuum too high -- waiting up to 40 seconds @14:41:03.64 SS.reconfigure :exhale @14:41:03.70 SS.hold @14:41:11.77 Intake @15.9psia, vacuum from 10.0 to 13.0psi @14:41:11.79 SS.reconfigure :inhaling 1.76ml/s @14:41:11.84 SS.seek 25ml @14:41:19.24 Vacuum too high -- waiting up to 40 seconds @14:41:19.26 SS.reconfigure :exhale @14:41:19.31 SS.hold @14:41:26.11 Intake @16.2psia, vacuum from 10.0 to 13.0psi @14:41:26.13 SS.reconfigure :inhaling 1.41ml/s @14:41:26.18 SS.seek 25ml @14:41:26.81 Stroke completed -- waiting for pressure to equalize ... @14:41:26.82 SS.reconfigure :exhale @14:41:27.32 SS.hold @14:41:35.92 Intake.select :close @14:41:36.07 SSV.dial :exhaust @14:41:36.85 Exhaust.select :open @14:41:36.96 SS.seek 0ml @14:41:45.67 Exhaust.select :close @14:41:45.79 SSV.dial CBV @14:41:45.81 CBV.dial SSV @14:41:46.63 Allowing 130s for 25.0ml of remaining 975.0ml @14:41:46.64 Intake.select :open @14:41:48.81 Intake @15.9psia, vacuum from 10.0 to 13.0psi @14:41:48.83 SS.reconfigure :inhaling 1.41ml/s @14:41:48.88 SS.seek 25ml @14:42:02.96 Vacuum too high -- waiting up to 40 seconds @14:42:02.98 SS.reconfigure :exhale @14:42:03.03 SS.hold @14:42:09.11 Intake @16.7psia, vacuum from 10.0 to 13.0psi @14:42:09.13 SS.reconfigure :inhaling 1.13ml/s @14:42:09.18 SS.seek 25ml @14:42:17.67 Stroke completed -- waiting for pressure to equalize ... @14:42:17.68 SS.reconfigure :exhale @14:42:17.73 SS.hold @14:42:32.14 Intake.select :close @14:42:32.30 SSV.dial :exhaust @14:42:33.08 Exhaust.select :open @14:42:33.19 SS.seek 0ml @14:42:42.00 Exhaust.select :close @14:42:42.11 SSV.dial CBV @14:42:42.14 CBV.dial SSV @14:42:42.92 Allowing 130s for 25.0ml of remaining 950.0ml @14:42:42.94 Intake.select :open @14:42:45.10 Intake @15.9psia, vacuum from 10.0 to 13.0psi @14:42:45.12 SS.reconfigure :inhaling 1.13ml/s @14:42:45.17 SS.seek 25ml @14:43:10.11 Stroke completed -- waiting for pressure to equalize ... @14:43:10.12 SS.reconfigure :exhale @14:43:10.17 SS.hold @14:43:26.49 Intake.select :close @14:43:26.65 SSV.dial :exhaust @14:43:27.44 Exhaust.select :open @14:43:27.55 SS.seek 0ml @14:43:36.67 Exhaust.select :close @14:43:36.78 SSV.dial CBV @14:43:36.82 CBV.dial SSV @14:43:37.58 Allowing 130s for 25.0ml of remaining 925.0ml @14:43:37.59 Intake.select :open @14:43:39.76 Intake @15.9psia, vacuum from 10.0 to 13.0psi @14:43:39.78 SS.reconfigure :inhaling 1.13ml/s @14:43:39.83 SS.seek 25ml @14:43:59.28 recoverPRVreagent08 @14:43:59.29 PTV.dial :mixing @14:44:00.05 PBV.dial :waste1 @14:44:00.43 delay 30 seconds @14:44:04.33 Stroke completed -- waiting for pressure to equalize ... @14:44:04.34 SS.reconfigure :exhale @14:44:04.39 SS.hold @14:44:18.78 Intake.select :close @14:44:18.94 SSV.dial :exhaust @14:44:19.70 Exhaust.select :open @14:44:19.82 SS.seek 0ml @14:44:28.98 Exhaust.select :close @14:44:29.10 SSV.dial CBV @14:44:29.13 CBV.dial SSV @14:44:29.90 Allowing 130s for 25.0ml of remaining 900.0ml @14:44:29.92 Intake.select :open @14:44:30.44 PBV.dial between PSV and waste1 @14:44:30.82 PSV.dial :air @14:44:31.41 PS.reconfigure :fast @14:44:31.46 PS.seek 2.5ml @14:44:32.09 Intake @15.9psia, vacuum from 10.0 to 13.0psi @14:44:32.11 SS.reconfigure :inhaling 1.13ml/s @14:44:32.16 SS.seek 25ml @14:44:55.50 PSV.dial :mixloop @14:44:55.96 delay 2 seconds @14:44:56.57 Stroke completed -- waiting for pressure to equalize ... @14:44:56.59 SS.reconfigure :exhale @14:44:56.63 SS.hold @14:44:57.97 PSV.dial :air @14:44:58.61 delay 2 seconds @14:45:00.69 PSV.dial :mixloop @14:45:01.14 delay 2 seconds @14:45:03.16 PSV.dial :air @14:45:03.76 PS.seek 0ml @14:45:13.46 Intake.select :close @14:45:13.61 SSV.dial :exhaust @14:45:14.37 Exhaust.select :open @14:45:14.49 SS.seek 0ml @14:45:23.45 Exhaust.select :close @14:45:23.56 SSV.dial CBV @14:45:23.60 CBV.dial SSV @14:45:24.34 Allowing 130s for 25.0ml of remaining 875.0ml @14:45:24.35 Intake.select :open @14:45:26.52 Intake @15.8psia, vacuum from 10.0 to 13.0psi @14:45:26.54 SS.reconfigure :inhaling 1.13ml/s @14:45:26.59 SS.seek 25ml @14:45:27.38 washCycleDA #1 of 2 @14:45:27.39 PSR.select :air @14:45:27.41 PSV.dial PSR @14:45:28.76 Pulling 0.5ml of PSR.wash @14:45:28.77 PSR.select :wash @14:45:28.89 PS.reconfigure :default @14:45:28.93 PS.seek 0.5ml @14:45:34.84 delay 5 seconds @14:45:39.86 PSR.select :air @14:45:39.98 PS.seek 2ml @14:45:50.92 Stroke completed -- waiting for pressure to equalize ... @14:45:50.93 SS.reconfigure :exhale @14:45:51.36 SS.hold @14:45:56.12 PTV.dial :puck @14:45:56.88 PSV.dial PTV @14:45:57.64 PBV.dial :waste1 @14:45:58.07 PS.seek 1.5ml @14:46:04.11 delay 3 seconds @14:46:05.82 Intake.select :close @14:46:05.98 SSV.dial :exhaust @14:46:06.75 Exhaust.select :open @14:46:06.86 SS.seek 0ml @14:46:07.15 PBV.dial between PSV and waste1 @14:46:07.51 PS.seek 0ml @14:46:15.84 Exhaust.select :close @14:46:16.41 SSV.dial CBV @14:46:16.44 CBV.dial SSV @14:46:17.21 Allowing 130s for 25.0ml of remaining 850.0ml @14:46:17.23 Intake.select :open @14:46:19.40 Intake @15.8psia, vacuum from 10.0 to 13.0psi @14:46:19.42 SS.reconfigure :inhaling 1.13ml/s @14:46:19.47 SS.seek 25ml @14:46:23.66 delay 2:00 @14:46:42.74 Vacuum too high -- waiting up to 40 seconds @14:46:42.76 SS.reconfigure :exhale @14:46:42.81 SS.hold @14:46:50.41 Intake @15.3psia, vacuum from 9.8 to 12.8psi @14:46:50.43 SS.reconfigure :inhaling 0.91ml/s @14:46:50.48 SS.seek 25ml @14:46:51.91 Stroke completed -- waiting for pressure to equalize ... @14:46:51.93 SS.reconfigure :exhale @14:46:51.97 SS.hold @14:47:01.07 Intake.select :close @14:47:01.23 SSV.dial :exhaust @14:47:01.99 Exhaust.select :open @14:47:02.11 SS.seek 0ml @14:47:10.88 Exhaust.select :close @14:47:11.00 SSV.dial CBV @14:47:11.04 CBV.dial SSV @14:47:11.79 Allowing 130s for 25.0ml of remaining 825.0ml @14:47:11.80 Intake.select :open @14:47:13.97 Intake @15.7psia, vacuum from 10.0 to 13.0psi @14:47:13.99 SS.reconfigure :inhaling 0.91ml/s @14:47:14.04 SS.seek 25ml @14:47:43.88 Stroke completed -- waiting for pressure to equalize ... @14:47:43.89 SS.reconfigure :exhale @14:47:43.94 SS.hold @14:47:56.74 Intake.select :close @14:47:56.89 SSV.dial :exhaust @14:47:57.67 Exhaust.select :open @14:47:57.78 SS.seek 0ml @14:48:06.57 Exhaust.select :close @14:48:06.69 SSV.dial CBV @14:48:06.72 CBV.dial SSV @14:48:07.49 Allowing 130s for 25.0ml of remaining 800.0ml @14:48:07.51 Intake.select :open @14:48:09.67 Intake @15.6psia, vacuum from 10.0 to 13.0psi @14:48:09.69 SS.reconfigure :inhaling 0.91ml/s @14:48:09.74 SS.seek 25ml @14:48:23.68 recoverPSRreagent08 @14:48:23.69 PTV.dial :puck @14:48:23.76 PBV.dial :waste1 @14:48:24.14 delay 30 seconds @14:48:39.71 Stroke completed -- waiting for pressure to equalize ... @14:48:39.72 SS.reconfigure :exhale @14:48:39.77 SS.hold @14:48:52.93 Intake.select :close @14:48:53.09 SSV.dial :exhaust @14:48:53.87 Exhaust.select :open @14:48:53.99 SS.seek 0ml @14:48:54.16 PBV.dial between PSV and waste1 @14:48:54.53 PSR.select :air @14:48:54.55 PSV.dial PSR @14:48:55.32 PS.reconfigure :fast @14:48:55.37 PS.seek 2.5ml @14:49:03.02 Exhaust.select :close @14:49:03.14 SSV.dial CBV @14:49:03.18 CBV.dial SSV @14:49:03.93 Allowing 130s for 25.0ml of remaining 775.0ml @14:49:03.94 Intake.select :open @14:49:06.11 Intake @15.6psia, vacuum from 10.0 to 13.0psi @14:49:06.13 SS.reconfigure :inhaling 0.91ml/s @14:49:06.18 SS.seek 25ml @14:49:19.53 PSV.dial PTV @14:49:20.30 delay 5 seconds @14:49:25.32 PSV.dial PSR @14:49:26.09 delay 5 seconds @14:49:31.11 PSV.dial PTV @14:49:31.88 delay 5 seconds @14:49:35.66 Stroke completed -- waiting for pressure to equalize ... @14:49:35.68 SS.reconfigure :exhale @14:49:35.72 SS.hold @14:49:36.89 PSV.dial :air @14:49:37.66 PS.seek 0ml @14:49:52.24 Intake.select :close @14:49:52.40 SSV.dial :exhaust @14:49:53.16 Exhaust.select :open @14:49:53.28 SS.seek 0ml @14:50:01.41 washCycleDA #2 of 2 @14:50:01.42 PSR.select :air @14:50:01.43 PSV.dial PSR @14:50:02.09 Exhaust.select :close @14:50:02.20 SSV.dial CBV @14:50:02.24 CBV.dial SSV @14:50:02.78 Pulling 0.5ml of PSR.wash @14:50:02.79 PSR.select :wash @14:50:02.91 PS.reconfigure :default @14:50:02.95 PS.seek 0.5ml @14:50:03.02 Allowing 130s for 25.0ml of remaining 750.0ml @14:50:03.04 Intake.select :open @14:50:05.66 Intake @15.3psia, vacuum from 9.8 to 12.8psi @14:50:05.68 SS.reconfigure :inhaling 0.91ml/s @14:50:05.73 SS.seek 25ml @14:50:08.87 delay 5 seconds @14:50:13.89 PSR.select :air @14:50:14.02 PS.seek 2ml @14:50:30.12 PTV.dial :puck @14:50:30.20 PSV.dial PTV @14:50:30.95 PBV.dial :waste1 @14:50:31.39 PS.seek 1.5ml @14:50:35.60 Stroke completed -- waiting for pressure to equalize ... @14:50:35.62 SS.reconfigure :exhale @14:50:35.67 SS.hold @14:50:37.37 delay 3 seconds @14:50:40.39 PBV.dial between PSV and waste1 @14:50:40.77 PS.seek 0ml @14:50:52.00 Intake.select :close @14:50:52.16 SSV.dial :exhaust @14:50:52.95 Exhaust.select :open @14:50:53.07 SS.seek 0ml @14:50:56.94 delay 2:00 @14:51:02.16 Exhaust.select :close @14:51:02.28 SSV.dial CBV @14:51:02.30 CBV.dial SSV @14:51:03.08 Allowing 130s for 25.0ml of remaining 725.0ml @14:51:03.10 Intake.select :open @14:51:05.26 Intake @15.4psia, vacuum from 9.9 to 12.9psi @14:51:05.28 SS.reconfigure :inhaling 0.91ml/s @14:51:05.33 SS.seek 25ml @14:51:34.63 Stroke completed -- waiting for pressure to equalize ... @14:51:34.64 SS.reconfigure :exhale @14:51:34.69 SS.hold @14:51:55.16 Intake.select :close @14:51:55.31 SSV.dial :exhaust @14:51:56.08 Exhaust.select :open @14:51:56.25 SS.seek 0ml @14:52:05.05 Exhaust.select :close @14:52:05.16 SSV.dial CBV @14:52:05.19 CBV.dial SSV @14:52:06.00 Allowing 130s for 25.0ml of remaining 700.0ml @14:52:06.02 Intake.select :open @14:52:08.19 Intake @15.3psia, vacuum from 9.8 to 12.8psi @14:52:08.21 SS.reconfigure :inhaling 0.91ml/s @14:52:08.26 SS.seek 25ml @14:52:30.51 Vacuum too high -- waiting up to 40 seconds @14:52:30.53 SS.reconfigure :exhale @14:52:30.58 SS.hold @14:52:40.55 Intake @15.5psia, vacuum from 10.0 to 13.0psi @14:52:40.57 SS.reconfigure :inhaling 0.73ml/s @14:52:40.62 SS.seek 25ml @14:52:50.28 Stroke completed -- waiting for pressure to equalize ... @14:52:50.30 SS.reconfigure :exhale @14:52:50.34 SS.hold @14:52:56.97 recoverPSRreagent08 @14:52:56.98 PTV.dial :puck @14:52:57.06 PBV.dial :waste1 @14:52:57.43 delay 30 seconds @14:53:03.02 Intake.select :close @14:53:03.18 SSV.dial :exhaust @14:53:04.01 Exhaust.select :open @14:53:04.13 SS.seek 0ml @14:53:13.13 Exhaust.select :close @14:53:13.24 SSV.dial CBV @14:53:13.27 CBV.dial SSV @14:53:14.05 Allowing 130s for 25.0ml of remaining 675.0ml @14:53:14.06 Intake.select :open @14:53:16.23 Intake @15.2psia, vacuum from 9.7 to 12.7psi @14:53:16.25 SS.reconfigure :inhaling 0.73ml/s @14:53:16.30 SS.seek 25ml @14:53:27.46 PBV.dial between PSV and waste1 @14:53:27.84 PSR.select :air @14:53:27.86 PSV.dial PSR @14:53:28.62 PS.reconfigure :fast @14:53:28.67 PS.seek 2.5ml @14:53:52.28 Stroke completed -- waiting for pressure to equalize ... @14:53:52.30 SS.reconfigure :exhale @14:53:52.34 SS.hold @14:53:52.80 PSV.dial PTV @14:53:53.69 delay 5 seconds @14:53:58.71 PSV.dial PSR @14:53:59.46 delay 5 seconds @14:54:04.48 PSV.dial PTV @14:54:05.24 delay 5 seconds @14:54:10.26 PSV.dial :air @14:54:11.03 PS.seek 0ml @14:54:12.80 Intake.select :close @14:54:12.95 SSV.dial :exhaust @14:54:13.73 Exhaust.select :open @14:54:13.84 SS.seek 0ml @14:54:22.88 Exhaust.select :close @14:54:22.99 SSV.dial CBV @14:54:23.02 CBV.dial SSV @14:54:23.80 Allowing 130s for 25.0ml of remaining 650.0ml @14:54:23.81 Intake.select :open @14:54:25.98 Intake @15.2psia, vacuum from 9.7 to 12.7psi @14:54:26.00 SS.reconfigure :inhaling 0.73ml/s @14:54:26.05 SS.seek 25ml @14:54:34.84 getPMLReagent08 @14:54:34.85 PRV.dial :air @14:54:35.32 delay 5 seconds @14:54:40.34 PTV.dial :PRVmixing @14:54:41.66 delay 5 seconds @14:54:46.68 PRV.dial :ab2 @14:54:47.15 PTV.dial :PRVmixing @14:54:47.16 PSV.dial :mixloop @14:54:47.64 PS.reconfigure :default @14:54:47.68 PS.seek 0.3ml @14:54:51.65 delay 2 seconds @14:54:53.66 PRV.dial :air @14:54:54.19 PS.seek 0.55ml @14:54:54.88 Vacuum too high -- waiting up to 40 seconds @14:54:54.90 SS.reconfigure :exhale @14:54:54.95 SS.hold @14:54:57.68 addPMLReagent08 @14:54:57.69 PSV.dial :air @14:54:58.22 PS.seek 2ml @14:55:06.50 Intake @15.0psia, vacuum from 9.5 to 12.5psi @14:55:06.52 SS.reconfigure :inhaling 0.59ml/s @14:55:06.57 SS.seek 25ml @14:55:13.96 PSV.dial :PML @14:55:14.43 PTV.dial :mixing @14:55:15.19 PBV.dial :waste1 @14:55:15.63 PS.seek 1.85ml @14:55:15.70 Stroke completed -- waiting for pressure to equalize ... @14:55:15.71 SS.reconfigure :exhale @14:55:15.76 SS.hold @14:55:17.96 PBV.dial between PSV and waste1 @14:55:18.34 PS.seek 0ml @14:55:28.70 Intake.select :close @14:55:28.86 SSV.dial :exhaust @14:55:29.65 Exhaust.select :open @14:55:29.76 SS.seek 0ml @14:55:38.02 delay 2 seconds @14:55:38.77 Exhaust.select :close @14:55:38.88 SSV.dial CBV @14:55:38.92 CBV.dial SSV @14:55:39.67 Allowing 130s for 25.0ml of remaining 625.0ml @14:55:39.69 Intake.select :open @14:55:40.04 delay 8:00 @14:55:41.85 Intake @15.2psia, vacuum from 9.7 to 12.7psi @14:55:41.87 SS.reconfigure :inhaling 0.59ml/s @14:55:41.92 SS.seek 25ml @14:56:26.32 Stroke completed -- waiting for pressure to equalize ... @14:56:26.34 SS.reconfigure :exhale @14:56:26.38 SS.hold @14:56:51.00 Intake.select :close @14:56:51.16 SSV.dial :exhaust @14:56:51.94 Exhaust.select :open @14:56:52.05 SS.seek 0ml @14:57:00.89 Exhaust.select :close @14:57:01.00 SSV.dial CBV @14:57:01.04 CBV.dial SSV @14:57:01.78 Allowing 130s for 25.0ml of remaining 600.0ml @14:57:01.79 Intake.select :open @14:57:03.96 Intake @15.1psia, vacuum from 9.6 to 12.6psi @14:57:03.98 SS.reconfigure :inhaling 0.59ml/s @14:57:04.03 SS.seek 25ml @14:57:43.69 Vacuum too high -- waiting up to 40 seconds @14:57:43.71 SS.reconfigure :exhale @14:57:43.76 SS.hold @14:57:58.67 Intake @15.3psia, vacuum from 9.8 to 12.8psi @14:57:58.69 SS.reconfigure :inhaling 0.47ml/s @14:57:58.74 SS.seek 25ml @14:58:05.35 Stroke completed -- waiting for pressure to equalize ... @14:58:05.37 SS.reconfigure :exhale @14:58:05.41 SS.hold @14:58:18.20 Intake.select :close @14:58:18.46 SSV.dial :exhaust @14:58:19.23 Exhaust.select :open @14:58:19.35 SS.seek 0ml @14:58:28.17 Exhaust.select :close @14:58:28.28 SSV.dial CBV @14:58:28.30 CBV.dial SSV @14:58:29.11 Allowing 130s for 25.0ml of remaining 575.0ml @14:58:29.12 Intake.select :open @14:58:31.29 Intake @15.2psia, vacuum from 9.7 to 12.7psi @14:58:31.31 SS.reconfigure :inhaling 0.47ml/s @14:58:31.36 SS.seek 25ml @14:59:26.96 Stroke completed -- waiting for pressure to equalize ... @14:59:26.97 SS.reconfigure :exhale @14:59:27.02 SS.hold @14:59:53.67 Intake.select :close @14:59:53.83 SSV.dial :exhaust @14:59:54.63 Exhaust.select :open @14:59:54.74 SS.seek 0ml @15:00:03.81 Exhaust.select :close @15:00:03.92 SSV.dial CBV @15:00:03.95 CBV.dial SSV @15:00:04.73 Allowing 130s for 25.0ml of remaining 550.0ml @15:00:04.74 Intake.select :open @15:00:06.90 Intake @15.2psia, vacuum from 9.7 to 12.7psi @15:00:06.92 SS.reconfigure :inhaling 0.47ml/s @15:00:06.97 SS.seek 25ml @15:01:02.13 Stroke completed -- waiting for pressure to equalize ... @15:01:02.14 SS.reconfigure :exhale @15:01:02.19 SS.hold @15:01:33.17 Intake.select :close @15:01:33.80 SSV.dial :exhaust @15:01:34.57 Exhaust.select :open @15:01:34.69 SS.seek 0ml @15:01:43.52 Exhaust.select :close @15:01:43.64 SSV.dial CBV @15:01:43.68 CBV.dial SSV @15:01:44.43 Allowing 130s for 25.0ml of remaining 525.0ml @15:01:44.44 Intake.select :open @15:01:46.61 Intake @15.3psia, vacuum from 9.8 to 12.8psi @15:01:46.63 SS.reconfigure :inhaling 0.47ml/s @15:01:46.68 SS.seek 25ml @15:02:36.87 Vacuum too high -- waiting up to 40 seconds @15:02:36.89 SS.reconfigure :exhale @15:02:36.94 SS.hold @15:02:55.44 Intake @15.2psia, vacuum from 9.7 to 12.7psi @15:02:55.46 SS.reconfigure :inhaling 0.38ml/s @15:02:55.51 SS.seek 25ml @15:03:02.61 Stroke completed -- waiting for pressure to equalize ... @15:03:02.63 SS.reconfigure :exhale @15:03:02.67 SS.hold @15:03:21.06 Intake.select :close @15:03:21.62 SSV.dial :exhaust @15:03:22.46 Exhaust.select :open @15:03:22.58 SS.seek 0ml @15:03:31.38 Exhaust.select :close @15:03:31.50 SSV.dial CBV @15:03:31.52 CBV.dial SSV @15:03:32.30 Allowing 130s for 25.0ml of remaining 500.0ml @15:03:32.32 Intake.select :open @15:03:34.49 Intake @15.2psia, vacuum from 9.7 to 12.7psi @15:03:34.51 SS.reconfigure :inhaling 0.38ml/s @15:03:34.56 SS.seek 25ml @15:03:40.06 recoverPRVreagent08 @15:03:40.07 PTV.dial :mixing @15:03:40.15 PBV.dial :waste1 @15:03:40.53 delay 30 seconds @15:04:10.55 PBV.dial between PSV and waste1 @15:04:10.93 PSV.dial :air @15:04:11.53 PS.reconfigure :fast @15:04:11.58 PS.seek 2.5ml @15:04:35.48 PSV.dial :mixloop @15:04:35.93 delay 2 seconds @15:04:37.95 PSV.dial :air @15:04:38.42 delay 2 seconds @15:04:40.44 PSV.dial :mixloop @15:04:40.90 delay 2 seconds @15:04:42.92 PSV.dial :air @15:04:43.39 PS.seek 0ml @15:04:43.58 Stroke completed -- waiting for pressure to equalize ... @15:04:43.59 SS.reconfigure :exhale @15:04:43.64 SS.hold @15:05:07.10 washCycleDA #1 of 3 @15:05:07.11 PSR.select :air @15:05:07.13 PSV.dial PSR @15:05:08.48 Pulling 0.5ml of PSR.wash @15:05:08.49 PSR.select :wash @15:05:08.61 PS.reconfigure :default @15:05:08.66 PS.seek 0.5ml @15:05:14.57 delay 5 seconds @15:05:18.43 Intake.select :close @15:05:18.58 SSV.dial :exhaust @15:05:19.35 Exhaust.select :open @15:05:19.46 SS.seek 0ml @15:05:19.59 PSR.select :air @15:05:19.70 PS.seek 2ml @15:05:28.33 Exhaust.select :close @15:05:28.45 SSV.dial CBV @15:05:28.48 CBV.dial SSV @15:05:29.25 Allowing 130s for 25.0ml of remaining 475.0ml @15:05:29.27 Intake.select :open @15:05:31.43 Intake @15.2psia, vacuum from 9.7 to 12.7psi @15:05:31.45 SS.reconfigure :inhaling 0.38ml/s @15:05:31.50 SS.seek 25ml @15:05:35.82 PTV.dial :puck @15:05:36.57 PSV.dial PTV @15:05:37.32 PBV.dial :waste1 @15:05:37.76 PS.seek 1.5ml @15:05:43.70 delay 3 seconds @15:05:46.72 PBV.dial between PSV and waste1 @15:05:47.09 PS.seek 0ml @15:06:03.29 delay 2:00 @15:06:31.15 Vacuum too high -- waiting up to 40 seconds @15:06:31.17 SS.reconfigure :exhale @15:06:31.22 SS.hold @15:06:53.76 Intake @15.8psia, vacuum from 10.0 to 13.0psi @15:06:53.78 SS.reconfigure :inhaling 0.31ml/s @15:06:53.83 SS.seek 25ml @15:07:07.19 Stroke completed -- waiting for pressure to equalize ... @15:07:07.20 SS.reconfigure :exhale @15:07:07.25 SS.hold @15:07:30.20 Intake.select :close @15:07:30.37 SSV.dial :exhaust @15:07:31.13 Exhaust.select :open @15:07:31.24 SS.seek 0ml @15:07:40.27 Exhaust.select :close @15:07:40.38 SSV.dial CBV @15:07:40.42 CBV.dial SSV @15:07:41.17 Allowing 130s for 25.0ml of remaining 450.0ml @15:07:41.19 Intake.select :open @15:07:43.36 Intake @15.2psia, vacuum from 9.7 to 12.7psi @15:07:43.38 SS.reconfigure :inhaling 0.31ml/s @15:07:43.43 SS.seek 25ml @15:08:03.32 recoverPSRreagent08 @15:08:03.33 PTV.dial :puck @15:08:03.34 PBV.dial :waste1 @15:08:03.71 delay 30 seconds @15:08:33.74 PBV.dial between PSV and waste1 @15:08:34.11 PSR.select :air @15:08:34.13 PSV.dial PSR @15:08:34.90 PS.reconfigure :fast @15:08:34.95 PS.seek 2.5ml @15:08:59.10 PSV.dial PTV @15:08:59.87 delay 5 seconds @15:09:04.89 PSV.dial PSR @15:09:05.77 delay 5 seconds @15:09:10.79 PSV.dial PTV @15:09:11.54 delay 5 seconds @15:09:13.04 Stroke completed -- waiting for pressure to equalize ... @15:09:13.06 SS.reconfigure :exhale @15:09:13.10 SS.hold @15:09:16.61 PSV.dial :air @15:09:17.40 PS.seek 0ml @15:09:40.88 washCycleDA #2 of 3 @15:09:40.89 PSR.select :air @15:09:40.91 PSV.dial PSR @15:09:42.22 Pulling 0.5ml of PSR.wash @15:09:42.23 PSR.select :wash @15:09:42.35 PS.reconfigure :default @15:09:42.39 PS.seek 0.5ml @15:09:48.28 delay 5 seconds @15:09:51.20 SS.reconfigure :default @15:09:51.25 SS.hold @15:09:51.32 Intake.select :close @15:09:51.47 ShallowSampler::Clogged in WCR -- Filtering too Slowly after 575.0ml @15:09:51.49 SSV.dial :exhaust @15:09:52.26 Exhaust.select :open @15:09:52.38 SS.reconfigure :exhale @15:09:52.43 SS.seek 0ml @15:09:53.30 PSR.select :air @15:09:53.90 PS.seek 2ml @15:10:01.50 Exhaust.select :close @15:10:01.61 Sampled 575.0ml @15:10:01.63 Drying puck before fixing for 5:00 @15:10:01.64 CBV.dial :waste2b @15:10:02.41 delay 1:00 @15:10:10.10 PTV.dial :puck @15:10:10.11 PSV.dial PTV @15:10:10.86 PBV.dial :waste1 @15:10:11.30 PS.seek 1.5ml @15:10:17.29 delay 3 seconds @15:10:20.31 PBV.dial between PSV and waste1 @15:10:20.67 PS.seek 0ml @15:10:36.85 delay 2:00 @15:11:02.43 CSV.dial :air @15:11:02.90 CS.reconfigure :fast @15:11:02.95 CS.seek 10ml @15:11:27.48 Pumping wcr puck from tube 3 up to 35psi... @15:11:27.49 CBV.dial SSV @15:11:28.76 CBV.dial between CSV and waste2b @15:11:29.74 CSV.dial between air and CTV @15:11:30.06 CS.seek 7.346ml @15:11:36.91 CTV.dial CSV,avoiding: :air @15:11:36.94 CSV.dial CTV @15:11:37.82 CS.reconfigure :pressurize @15:11:37.87 CS.seek 0ml @15:11:56.74 CBV.dial :waste2b @15:11:57.07 CS.reconfigure :default @15:11:57.12 CS.seek 0ml @15:11:58.09 delay 1:00 @15:12:36.87 recoverPSRreagent08 @15:12:36.89 PTV.dial :puck @15:12:36.90 PBV.dial :waste1 @15:12:37.25 delay 30 seconds @15:12:58.11 CTV.dial :air,avoiding: :intake @15:12:59.44 Fix #1 of 2 (with RNAlater) for 2:30 @15:12:59.46 CSV.dial CSR @15:12:59.91 Pulling 2ml of CSR.RNAlater @15:12:59.92 CSR.select :RNAlater @15:13:00.04 CS.seek 2ml @15:13:06.19 delay 5 seconds @15:13:07.27 PBV.dial between PSV and waste1 @15:13:07.63 PSR.select :air @15:13:07.64 PSV.dial PSR @15:13:08.41 PS.reconfigure :fast @15:13:08.46 PS.seek 2.5ml @15:13:11.21 CSR.select :air @15:13:11.33 CS.reconfigure :fast @15:13:11.85 CS.seek 10ml @15:13:31.75 Pumping wcr puck from tube 3 up to 35psi... @15:13:31.77 CBV.dial SSV @15:13:32.41 PSV.dial PTV @15:13:33.02 CBV.dial between CSV and waste2b @15:13:33.17 delay 5 seconds @15:13:34.00 CSV.dial between air and CTV @15:13:34.60 CS.seek 7.311ml @15:13:38.19 PSV.dial PSR @15:13:38.95 delay 5 seconds @15:13:41.66 CTV.dial CSV,avoiding: :air @15:13:41.71 CSV.dial CTV @15:13:43.05 CS.reconfigure :pressurize @15:13:43.10 CS.seek 0ml @15:13:43.97 PSV.dial PTV @15:13:44.73 delay 5 seconds @15:13:49.75 PSV.dial :air @15:13:50.52 PS.seek 0ml @15:13:58.52 delay 2:30 @15:14:13.91 washCycleDA #3 of 3 @15:14:13.92 PSR.select :air @15:14:13.94 PSV.dial PSR @15:14:15.26 Pulling 0.5ml of PSR.wash @15:14:15.28 PSR.select :wash @15:14:15.39 PS.reconfigure :default @15:14:15.44 PS.seek 0.5ml @15:14:21.31 delay 5 seconds @15:14:26.33 PSR.select :air @15:14:26.45 PS.seek 2ml @15:14:42.56 PTV.dial :puck @15:14:42.57 PSV.dial PTV @15:14:43.33 PBV.dial :waste1 @15:14:43.76 PS.seek 1.5ml @15:14:49.75 delay 3 seconds @15:14:52.77 PBV.dial between PSV and waste1 @15:14:53.14 PS.seek 0ml @15:15:09.25 delay 2:00 @15:16:28.55 CBV.dial :waste2b @15:16:28.88 CS.reconfigure :default @15:16:28.93 CS.seek 0ml @15:16:33.40 delay 1:00 @15:17:09.27 recoverPSRreagent08 @15:17:09.29 PTV.dial :puck @15:17:09.30 PBV.dial :waste1 @15:17:09.67 delay 30 seconds @15:17:33.43 CTV.dial :air,avoiding: :intake @15:17:34.77 Fix #2 of 2 (with RNAlater) for 2:30 @15:17:34.78 CSV.dial CSR @15:17:35.21 Pulling 2ml of CSR.RNAlater @15:17:35.22 CSR.select :RNAlater @15:17:35.34 CS.seek 2ml @15:17:39.69 PBV.dial between PSV and waste1 @15:17:40.09 PSR.select :air @15:17:40.11 PSV.dial PSR @15:17:40.88 PS.reconfigure :fast @15:17:40.93 PS.seek 2.5ml @15:17:41.74 delay 5 seconds @15:17:46.76 CSR.select :air @15:17:46.88 CS.reconfigure :fast @15:17:46.93 CS.seek 10ml @15:18:04.84 PSV.dial PTV @15:18:05.61 delay 5 seconds @15:18:06.68 Pumping wcr puck from tube 3 up to 35psi... @15:18:06.70 CBV.dial SSV @15:18:07.95 CBV.dial between CSV and waste2b @15:18:08.93 CSV.dial between air and CTV @15:18:09.53 CS.seek 7.259ml @15:18:10.62 PSV.dial PSR @15:18:11.37 delay 5 seconds @15:18:16.39 PSV.dial PTV @15:18:16.69 CTV.dial CSV,avoiding: :air @15:18:16.72 CSV.dial CTV @15:18:17.15 delay 5 seconds @15:18:18.11 CS.reconfigure :pressurize @15:18:18.17 CS.seek 0ml @15:18:22.17 PSV.dial :air @15:18:22.94 PS.seek 0ml @15:18:32.89 delay 2:30 @15:18:46.21 Gate.power :camera,:ON @15:18:46.23 Mixing DA Substrates @15:18:46.24 PSR.select :air @15:18:46.26 PSV.dial PSR @15:18:47.69 Pulling 0.25ml of PSR.sub1 @15:18:47.71 PSR.select :sub1 @15:18:47.93 PS.reconfigure :default @15:18:48.06 PS.seek 0.25ml @15:18:51.52 delay 5 seconds @15:18:56.55 Pulling 0.25ml of PSR.sub2 @15:18:56.56 PSR.select :sub2 @15:18:56.68 PS.seek 0.5ml @15:19:00.03 delay 5 seconds @15:19:05.05 PSR.select :air @15:19:05.16 PS.seek 2ml @15:19:21.27 PTV.dial :puck @15:19:21.29 PSV.dial PTV @15:19:22.04 PBV.dial :waste1 @15:19:22.48 PS.seek 1.5ml @15:19:28.46 delay 3 seconds @15:19:31.48 PBV.dial between PSV and waste1 @15:19:31.86 PS.seek 0ml @15:19:47.99 delay 10 seconds @15:19:58.01 recoverPSRreagent08 @15:19:58.02 PTV.dial :puck @15:19:58.03 PBV.dial :waste1 @15:19:58.42 delay 30 seconds @15:20:28.44 PBV.dial between PSV and waste1 @15:20:29.30 PSR.select :air @15:20:29.33 PSV.dial PSR @15:20:30.11 PS.reconfigure :fast @15:20:30.16 PS.seek 2.5ml @15:20:54.33 PSV.dial PTV @15:20:55.23 delay 5 seconds @15:21:00.25 PSV.dial PSR @15:21:01.01 delay 5 seconds @15:21:02.91 CBV.dial :waste2b @15:21:03.26 CS.reconfigure :default @15:21:03.31 CS.seek 0ml @15:21:06.03 PSV.dial PTV @15:21:06.82 delay 5 seconds @15:21:08.62 delay 1:00 @15:21:11.84 PSV.dial :air @15:21:12.62 PS.seek 0ml @15:21:36.59 Additional air clear @15:21:36.60 PSR.select :air @15:21:36.61 PSV.dial PSR @15:21:37.97 Pulling 2ml of PSR.air @15:21:37.99 PSR.select :air @15:21:38.01 PS.reconfigure :default @15:21:38.05 PS.seek 2ml @15:21:59.24 delay 5 seconds @15:22:04.26 PTV.dial :puck @15:22:04.28 PSV.dial PTV @15:22:05.04 PBV.dial :waste1 @15:22:05.47 PS.seek 1.5ml @15:22:08.64 CTV.dial :air,avoiding: :intake @15:22:11.43 delay 3 seconds @15:22:14.45 PBV.dial between PSV and waste1 @15:22:14.82 delay 1 second @15:22:15.84 PSV.dial :waste1 @15:22:16.92 PS.seek 0ml @15:22:33.14 Processing Puck Heater at 28C before imaging @15:22:33.15 PH.coast @15:22:33.27 Imaging da puck in Processing Clamp while storing it to tube 2 @15:22:33.44 SC.reconfigure :tight @15:22:33.49 SC.seek 2 @15:22:37.08 SC.configure :limp @15:22:37.30 Elbow.reconfigure :tight @15:22:37.34 Elbow.seek 2 @15:22:39.09 Forearm.reconfigure :tight @15:22:39.14 Forearm.seek 2 + 450 counts @15:22:40.16 SE.reconfigure :pick @15:22:40.22 SE.seek :beamBreak @15:22:42.81 SE.configure :default @15:22:43.47 Elbow.reconfigure :limp @15:22:43.52 Forearm.reconfigure :loose @15:22:43.57 Forearm.seek 2 @15:22:43.74 Elbow.hold @15:22:43.81 Elbow.reconfigure :tight @15:22:43.91 SE.seek 8.13 Pucks below Top Plate @15:22:44.36 Forearm.force -20 @15:22:44.41 Forearm.seek 2 + 319 counts @15:22:44.72 Forearm.reconfigure :tight @15:22:44.77 Forearm.seek 2 + 450 counts @15:22:45.00 SE.seek 9.13 Pucks below Top Plate @15:22:45.53 Forearm.reconfigure :loose @15:22:45.58 Forearm.seek :retract @15:22:46.53 Elbow.seek PC @15:22:49.00 Elbow.reconfigure :loose @15:22:49.11 Forearm.seek PC @15:22:50.07 Hand.close @15:22:50.71 PC.seek :open @15:22:55.51 Forearm.seek :retract @15:22:56.52 Elbow.reconfigure :tight @15:22:56.57 Elbow.seek 2 @15:22:59.28 Forearm.reconfigure :tight @15:22:59.33 Forearm.seek 2 + 450 counts @15:23:00.27 Hand.open @15:23:00.90 SE.seek 8.78 Pucks below Top Plate @15:23:01.16 SE.reconfigure :seal @15:23:01.21 SE.seek 7.82 Pucks below Top Plate @15:23:01.74 Lights, camera, ACTION! @15:23:01.77 PuckCamera: snap -bin=2,2 -auto /var/log/esp/da23oct2415h5ml.tif @15:23:20.32 SX HX9/16 (USB:11): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15.268 seconds /var/log/esp/da23oct2415h5ml.tif: TIFF Upload Complete @15:23:20.34 PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da23oct2415h5ml15s.tif @15:23:21.26 Waiting >20 seconds for da to relinquish FlushPuck @15:23:35.95 SX HX9/16 (USB:11): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15 seconds /var/log/esp/midres/da23oct2415h5ml15s.tif: TIFF Upload Complete @15:23:35.98 PuckCamera: snap -bin=2,2 20.0 /var/log/esp/midres/da23oct2415h5ml20s.tif @15:23:56.59 SX HX9/16 (USB:11): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 20 seconds /var/log/esp/midres/da23oct2415h5ml20s.tif: TIFF Upload Complete @15:23:56.61 DAfans.email "15.268 sec auto-exposure",Subject: "da23oct2415h5ml images!" @15:23:56.64 Gate.power :camera,:OFF @15:23:57.05 Elbow.seek 2 @15:23:57.94 Forearm.seek 2 + 450 counts @15:23:58.03 SE.reconfigure :gently @15:23:58.08 SE.seek 8.54 Pucks below Top Plate @15:23:58.73 Elbow.reconfigure :limp @15:23:58.80 Forearm.reconfigure :loose @15:23:58.85 Forearm.seek 2 @15:23:59.07 Elbow.hold @15:23:59.14 Elbow.reconfigure :tight @15:23:59.21 SE.reconfigure :default @15:23:59.25 SE.seek 8.72 Pucks below Top Plate @15:23:59.66 Forearm.force -20 @15:23:59.70 Forearm.seek 2 + 316 counts @15:24:00.05 Forearm.reconfigure :tight @15:24:00.10 Forearm.seek 2 + 450 counts @15:24:00.46 SE.seek :down @15:24:01.64 Forearm.reconfigure :loose @15:24:01.74 Forearm.seek :retract @15:24:02.62 SE.stop @15:24:02.92 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @15:24:03.48 Elbow.seek FlushPuck::Garage @15:24:05.38 Elbow.reconfigure :loose @15:24:05.59 Forearm.seek :garage @15:24:06.68 Hand.close @15:24:07.65 Forearm.seek :retract @15:24:08.72 Elbow.reconfigure :tight @15:24:08.83 Elbow.seek PC @15:24:10.22 Elbow.reconfigure :loose @15:24:11.52 Forearm.reconfigure :tight @15:24:11.60 Forearm.seek PC @15:24:13.32 PC.seek :closed @15:24:18.13 Processing Clamp onSpring/offSpring for 14/26 samples, on/off torque 1.62 @15:24:18.27 Hand.open @15:24:19.14 Forearm.reconfigure :loose @15:24:19.24 Forearm.seek :retract @15:24:20.29 PSR.select :air @15:24:20.36 Storing wcr puck in Collection Clamp to tube 2 @15:24:20.40 PSV.dial PSR @15:24:20.88 SC.reconfigure :tight @15:24:21.00 SC.seek 2 @15:24:21.11 Pulling 0.5ml of PSR.flush @15:24:21.17 PSR.select :flush @15:24:21.33 SC.configure :limp @15:24:21.65 PS.seek 0.5ml @15:24:21.93 Elbow.reconfigure :tight @15:24:22.07 Elbow.seek 2 @15:24:24.86 Forearm.reconfigure :tight @15:24:24.96 Forearm.seek 2 + 450 counts @15:24:26.18 SE.reconfigure :pick @15:24:26.29 SE.seek :beamBreak @15:24:27.66 delay 5 seconds @15:24:28.90 SE.configure :default @15:24:29.29 Elbow.reconfigure :limp @15:24:30.38 Forearm.reconfigure :loose @15:24:30.52 Forearm.seek 2 @15:24:30.82 Elbow.hold @15:24:30.97 Elbow.reconfigure :tight @15:24:31.26 SE.seek 9.11 Pucks below Top Plate @15:24:31.90 Forearm.force -20 @15:24:31.98 Forearm.seek 2 + 301 counts @15:24:32.54 Forearm.reconfigure :tight @15:24:32.64 Forearm.seek 2 + 450 counts @15:24:32.73 PSR.select :air @15:24:32.94 SE.seek 10.11 Pucks below Top Plate @15:24:33.10 PS.seek 2.5ml @15:24:33.52 upsync @15:24:33.58 Forearm.reconfigure :loose @15:24:33.64 Forearm.seek :retract @15:24:34.59 Elbow.seek CC @15:24:36.22 Elbow.reconfigure :loose @15:24:36.32 Forearm.seek CC @15:24:37.29 Hand.close @15:24:37.97 CC.seek :open @15:24:42.73 Forearm.seek :retract @15:24:43.68 Elbow.reconfigure :tight @15:24:43.73 Elbow.seek 2 @15:24:45.53 Elbow.reconfigure :limp @15:24:45.59 Forearm.seek 2 @15:24:46.57 Elbow.hold @15:24:46.64 Elbow.reconfigure :tight @15:24:46.69 Hand.open @15:24:47.90 Forearm.force -20 @15:24:47.94 Forearm.seek 2 + 313 counts @15:24:48.29 Forearm.reconfigure :tight @15:24:48.34 Forearm.seek 2 + 450 counts @15:24:48.67 SE.seek :down @15:24:49.81 Forearm.reconfigure :loose @15:24:49.86 Forearm.seek :retract @15:24:50.70 SE.stop @15:24:50.82
Starting finalPurge @15:24:54.39 delay 1 second @15:24:55.41 PRV.dial :waste2,avoiding: :ab2 @15:24:57.27 PTV.dial :PRVmixing @15:24:58.58 PSV.dial :mixloop @15:24:59.63 PS.seek :empty @15:25:10.76
Waiting >20 seconds for psFlush to relinquish FlushPuck @15:25:26.02 PSR.select :air @15:25:26.04 PSV.dial PSR @15:25:27.08 Pulling 0.5ml of PSR.flush @15:25:27.09 PSR.select :flush @15:25:27.21 PS.seek 0.5ml @15:25:33.08 delay 5 seconds @15:25:38.10 PSR.select :air @15:25:38.22 PS.seek 2.5ml @15:25:59.49 delay 1 second @15:26:00.50 PBV.dial :waste1 @15:26:00.88 PTV.dial :mixing @15:26:01.63 PSV.dial :mixloop @15:26:02.68 PS.seek :empty @15:26:29.20 PSV.dial PSR @15:26:30.25 PSR.select :flush @15:26:30.37 PS.seek 1ml @15:26:41.54 PSR.select :air @15:26:41.66 PS.seek 2.5ml @15:26:57.80 PSV.dial PTV @15:26:58.56 PTV.dial :puck @15:26:59.31 PBV.dial :waste1 @15:26:59.49 PS.seek 0ml @15:27:26.04 PSV.dial PSR @15:27:26.80 PSR.select :flush @15:27:26.92 PS.seek 0.5ml @15:27:33.37 PSR.select :air @15:27:33.52 PS.seek 2.5ml @15:27:54.83 PSV.dial PTV @15:27:55.70 PBV.dial PSV @15:27:56.27 PS.seek 0ml @15:28:22.61 PSV.dial PBV @15:28:23.07 PS.seek 2.5ml @15:28:49.42 PSV.dial :waste1 @15:28:50.16 PS.seek 0ml @15:29:16.76 PSV.dial PSR @15:29:17.23 PSR.select :air @15:29:17.26 PS.seek 2.5ml @15:29:43.60 PSV.dial PTV @15:29:44.46 PBV.dial PSV @15:29:44.66 PS.seek 0ml @15:30:10.98 PSV.dial PBV @15:30:11.44 PS.seek 2.5ml @15:30:37.76 PSV.dial :waste1 @15:30:38.50 PS.seek 0ml @15:31:04.88 PSV.dial PSR @15:31:05.35 PSR.select :flush @15:31:05.48 PS.seek 1ml @15:31:16.57 PSR.select :air @15:31:16.69 PS.seek 2.5ml @15:31:32.85 PSV.dial PTV @15:31:33.59 PTV.dial :puck @15:31:33.71 PBV.dial :waste1 @15:31:34.27 PS.seek 0ml @15:32:00.60 PSV.dial PSR @15:32:01.35 PSR.select :flush @15:32:01.47 PS.seek 0.5ml @15:32:07.44 PSR.select :air @15:32:07.56 PS.seek 2.5ml @15:32:28.85 PSV.dial PTV @15:32:29.60 PBV.dial PSV @15:32:30.17 PS.seek 0ml @15:32:56.48 PSV.dial PBV @15:32:56.95 PS.seek 2.5ml @15:33:23.32 PSV.dial :waste1 @15:33:24.06 PS.seek 0ml @15:33:50.57 PSV.dial PSR @15:33:51.04 PSR.select :air @15:33:51.06 PS.seek 2.5ml @15:34:17.82 PSV.dial PTV @15:34:18.74 PBV.dial PSV @15:34:18.93 PS.seek 0ml @15:34:45.35 PSV.dial PBV @15:34:45.81 PS.seek 2.5ml @15:35:12.13 PSV.dial :waste1 @15:35:12.87 PS.seek 0ml @15:35:39.39
Loading FlushPuck into Collection Clamp from Processing Clamp @15:35:39.57 Elbow.seek PC @15:35:42.07 Elbow.reconfigure :loose @15:35:42.17 Forearm.seek PC @15:35:43.12 Hand.close @15:35:43.70 PC.seek :open @15:35:48.41 Forearm.seek :retract @15:35:49.40 Elbow.reconfigure :tight @15:35:49.45 Elbow.seek CC @15:35:53.08 Elbow.reconfigure :loose @15:35:53.18 Forearm.reconfigure :tight @15:35:53.23 Forearm.seek CC @15:35:54.53 CC.seek :closed @15:35:59.06 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.40 @15:35:59.13 Hand.open @15:35:59.77 Forearm.reconfigure :loose @15:35:59.82 Forearm.seek :retract @15:36:00.81 Flushing the Collection Valve::Manifold @15:36:00.94 CSV.dial CSR @15:36:01.37 Pulling 5ml of CSR.flush CSR.select :flush @15:36:01.95 CS.seek 5ml @15:36:15.76 delay 5 seconds @15:36:20.78 CSR.select :air @15:36:20.94 CS.reconfigure :fast @15:36:20.99 CS.seek 7.5ml @15:36:27.72 CSV.dial :waste @15:36:29.01 CS.reconfigure :default @15:36:29.06 CS.seek 0ml @15:36:49.10 Flushing lower manifold's waste1 port @15:36:49.22 CSV.dial :flush @15:36:49.67 CS.reconfigure :fast @15:36:49.72 CS.seek 5ml @15:37:02.70 delay 5 seconds @15:37:07.71 CSV.dial :air @15:37:08.24 CS.seek 7ml @15:37:13.88 CSV.dial CTV @15:37:13.91
CTV.dial CSV @15:37:15.29 CBV.dial :waste1 @15:37:16.06 CS.seek 0ml @15:37:32.73 Flushing lower manifold's waste2 port @15:37:32.85 CSV.dial :flush @15:37:33.59 CS.seek 5ml @15:37:46.51 delay 5 seconds @15:37:51.53 CSV.dial :air @15:37:52.04 CS.seek 7ml @15:37:57.63 CSV.dial CTV @15:37:57.66
CTV.dial CSV @15:37:58.11 CBV.dial :waste2 @15:37:59.17 CS.seek 0ml @15:38:15.79 Flushing lower manifold's waste2b port @15:38:15.91 CSV.dial :flush @15:38:16.65 CS.seek 5ml @15:38:29.35 delay 5 seconds @15:38:34.37 CSV.dial :air @15:38:34.88 CS.seek 7ml @15:38:40.60 CSV.dial CTV @15:38:40.64
CTV.dial CSV @15:38:41.07 CBV.dial :waste2b @15:38:42.12 CS.seek 0ml @15:38:58.91 Disinfecting external Intake with kill @15:38:59.04 CSV.dial CSR @15:38:59.48 Pulling 8ml of CSR.kill CSR.select :kill @15:38:59.60 CS.seek 8ml @15:39:19.84 delay 5 seconds @15:39:24.97 CSR.select :air @15:39:25.09 CS.seek 10ml @15:39:30.69 CSV.dial CBV @15:39:30.72
CBV.dial CSV @15:39:31.79 CTV.dial :intake @15:39:32.57 Intake.select :open @15:39:32.68 CS.seek 2ml @15:39:51.63 Intake.select :close @15:39:51.73 CTV.dial :air @15:39:53.26 CS.reconfigure :default @15:39:53.31 CS.seek 0ml @15:39:59.22 CSV.dial CSR @15:40:00.24 Pulling 5ml of CSR.flush @15:40:00.25 CSR.select :flush @15:40:00.36 CS.seek 5ml @15:40:13.94 delay 5 seconds @15:40:18.96 CSR.select :air @15:40:19.10 CS.seek 10ml @15:40:32.78 CSV.dial CBV @15:40:33.79 CS.seek 0ml @15:41:00.22 Garaging FlushPuck in Collection Clamp @15:41:00.30 Elbow.reconfigure :tight @15:41:00.34 Elbow.seek CC @15:41:00.61 Elbow.reconfigure :loose @15:41:00.71 Forearm.seek CC @15:41:01.74 Hand.close @15:41:02.32 CC.seek :open @15:41:06.93 Forearm.seek :retract @15:41:07.98 Elbow.reconfigure :tight @15:41:08.03 Elbow.seek :garage @15:41:10.91 Elbow.reconfigure :loose @15:41:11.01 Forearm.reconfigure :tight @15:41:11.06 Forearm.seek :garage @15:41:12.15 Hand.open @15:41:12.88 Forearm.reconfigure :loose @15:41:12.93 Forearm.seek :retract @15:41:13.98 Completed [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] begun 13:49:41 @15:41:14.03 Asleep after completing DA_WCR_102423 assays @15:41:14.05 GoodNews.email "{1 => 33,\n 2 => 11,\n 3 => 22,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@15:38:51, 26% flow, 18.2C, 20% humidity, 14.6psia, 13.695V, 0.435A, 213.688Ah, 5.96W\nSampled 575.0ml",Subject: "Asleep after completing DA_WCR_102423 assays" @15:41:18.45 upsync + ESPwhich DA_WCR_102423b.rb + fn=DA_WCR_102423b.rb + IFS=: + set /home/esp/esp2/mission . + fullPath=/home/esp/esp2/mission/DA_WCR_102423b.rb + [ -f /home/esp/esp2/mission/DA_WCR_102423b.rb ] + realpath /home/esp/esp2/mission/DA_WCR_102423b.rb + return 0 + scriptPath=/home/esp/esp2local/mission/DA_WCR_102423b.rb + exec ruby /home/esp/esp2/lib/esp.rb /home/esp/esp2local/mission/DA_WCR_102423b.rb @17:15:29.77UTC24-Oct-23
ESP2 v3.42 I2C Gateway v3.20 for core bus @17:15:32.43 Configuration for short puck jake 1/11/21 by brent@mbari.org /home/esp/esp2/type/shallow/jake/configure.rb @17:15:36.24 Polling Can every 10:00 @17:15:36.32 Cleanly exited at 17:12:45UTC24-Oct-23 @17:15:36.37 Loading /home/esp/esp2local/mission/DA_WCR_102423b.rb mission @17:15:36.42 Preparing to sleep until + 00:00:05 @17:15:36.44 GoodNews.email "Starting DA_WCR_102423b w/pucks in tube 3\n{1 => 33,\n 2 => 11,\n 3 => 22,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@17:15:36, 27% flow, 17.9C, 21% humidity, 14.6psia, 13.764V, 0.230A, 214.031Ah, 3.17W",Subject: "DA_WCR_102423b begun" @17:15:36.51
Asleep til + 00:00:05 @17:15:36.53 Continuing at 17:15 @17:15:36.77 Gate.power :core,:OFF @17:15:36.78 Gate.power :raw,:OFF @17:15:36.79 Shutdown @17:15:39.55 GoodNews.email "Then, in tube 3: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_102423b.rb:14\n{1 => 33,\n 2 => 11,\n 3 => 22,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@17:15:36, 27% flow, 17.9C, 21% humidity, 14.6psia, 13.759V, 0.236A, 214.031Ah, 3.25W",Subject: "Asleep until 17:15" @17:15:40.94 upsync @17:15:41.42
Powering core bus @17:15:43.68 upsync @17:15:47.11
Gate.power :raw,:ON @17:15:48.04 CS.configure :default @17:15:48.10 PS.configure :default @17:15:48.14 SS.configure :default @17:15:48.19 CC.configure :default @17:15:48.24 PC.configure :default @17:15:48.31 Forearm.configure :loose @17:15:48.35 Elbow.configure :loose @17:15:48.42 SC.configure :loose @17:15:48.47 SE.configure :default @17:15:48.52 CH.configure :default @17:15:48.55 PH.configure :default @17:15:48.59 All dwarf controllers running firmware version 3.85 @17:15:48.70 Valve.reset @17:15:48.92 Intake.select :close @17:15:48.97 Exhaust.select :close @17:15:49.01 CTV.dial :air @17:15:49.12 PSV.dial :air @17:15:49.18 CSV.dial :waste1 @17:15:49.28
Forearm.home.seek :retract @17:15:49.65 SSV.dial :spit,avoiding: :exhaust @17:15:50.04 Hand.close @17:15:50.08
Elbow.reconfigure :loose @17:15:50.19 Elbow.jog 1500 @17:15:50.26 PS.home.seek 0ml @17:15:50.52 CS.reconfigure :fast @17:15:50.55 SS.home.seek 0ml @17:15:50.64 CS.home.seek 0ml @17:15:50.71
Elbow.reconfigure :loose @17:15:50.78 Elbow.home.seek :clear @17:15:54.14 SE.home.seek :down @17:15:54.74 SC.reconfigure :tight @17:15:54.79 SC.home.seek :initial @17:15:58.51 PS.stop @17:16:23.15
SC.configure :limp @17:16:23.20 Forearm.seek :retract @17:16:23.26 Elbow.seek :hammer @17:16:24.30 Forearm.reconfigure :tight @17:16:24.35 Forearm.seek retracted @17:16:25.05 PC.home.seek :open @17:16:25.14
CC.home.seek :open @17:16:28.51 Phase [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] @17:16:28.53 Notice.email "At 17:16:28 began in tube 3: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_102423b.rb:14\n{1 => 33,\n 2 => 11,\n 3 => 22,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@17:16:28, 27% flow, 17.9C, 21% humidity, 14.6psia, 13.720V, 0.272A, 214.037Ah, 3.74W",Subject: "Started DA_WCR_102423b phase initialPurge; da [1000, 1000] #da and wsr are run simultanelously" @17:16:28.55
Starting initialPurge @17:16:28.57 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @17:16:28.64 Hand.open @17:16:29.37 Elbow.reconfigure :tight @17:16:29.42 Elbow.seek FlushPuck::Garage @17:16:31.36 Elbow.reconfigure :loose @17:16:31.58 Forearm.reconfigure :loose @17:16:31.68 Forearm.seek :garage @17:16:32.68 Hand.close @17:16:32.81 upsync @17:16:33.49
Forearm.seek :retract @17:16:34.47 Elbow.reconfigure :tight @17:16:34.52 Elbow.seek CC @17:16:37.40 Elbow.reconfigure :loose @17:16:37.50 Forearm.reconfigure :tight @17:16:37.55 Forearm.seek CC @17:16:38.93 CC.seek :closed @17:16:43.55 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.45 @17:16:43.62 Hand.open @17:16:44.29 Forearm.reconfigure :loose @17:16:44.34 Forearm.seek :retract @17:16:45.36 Flushing the Collection Valve::Manifold @17:16:45.49 CSV.dial CSR @17:16:46.76 Pulling 5ml of CSR.flush @17:16:46.77 CSR.select :flush @17:16:46.89 CS.reconfigure :default @17:16:46.93 CS.seek 5ml @17:17:01.06 delay 5 seconds @17:17:06.07 CSR.select :air @17:17:06.24 CS.reconfigure :fast @17:17:06.29 CS.seek 7.5ml @17:17:13.09 CSV.dial :waste @17:17:14.37 CS.reconfigure :default @17:17:14.42 CS.seek 0ml @17:17:34.46 Purging external Intake @17:17:34.58 CSV.dial :flush @17:17:35.03 CS.reconfigure :fast @17:17:35.08 CS.seek 10ml @17:17:59.74 delay 5 seconds @17:18:04.76 CSV.dial CBV @17:18:04.80
CBV.dial CSV @17:18:05.54 CTV.dial :intake @17:18:06.32 Intake.select :open @17:18:06.43 CS.seek 0ml @17:18:30.12 Intake.select :close @17:18:30.22 CTV.dial :air @17:18:31.01 Flushing upper manifold @17:18:31.13 CSV.dial :flush @17:18:31.87 CS.seek 5ml @17:18:44.65 delay 5 seconds @17:18:49.67 CSV.dial :air @17:18:50.16 CS.seek 7.001ml @17:18:55.74 CSV.dial CBV @17:18:55.77
CBV.dial CSV @17:18:56.79 CTV.dial :air @17:18:56.81 CS.seek 0ml @17:19:13.43 delay 7 seconds @17:19:20.46 CS.seek 3ml @17:19:28.49 CSV.dial :waste @17:19:28.95 CS.seek 0ml @17:19:36.72 CSV.dial :flush @17:19:37.15 CS.seek 3ml @17:19:45.12 delay 5 seconds @17:19:50.13 CSV.dial :air @17:19:50.76 CS.seek 5ml @17:19:56.52 CSV.dial :air @17:19:56.60 CS.seek 0ml @17:20:08.90 delay 7 seconds @17:20:15.92 CS.seek 3ml @17:20:23.89 CSV.dial :waste @17:20:24.62 CS.seek 0ml @17:20:32.31 Garaging FlushPuck in Collection Clamp @17:20:32.37 Elbow.reconfigure :tight @17:20:32.42 Elbow.seek CC @17:20:32.67 Elbow.reconfigure :loose @17:20:32.77 Forearm.seek CC @17:20:33.80 Hand.close @17:20:34.48 CC.seek :open @17:20:39.54 Forearm.seek :retract @17:20:40.53 Elbow.reconfigure :tight @17:20:40.57 Elbow.seek :garage @17:20:43.56 Elbow.reconfigure :loose @17:20:43.66 Forearm.reconfigure :tight @17:20:43.71 Forearm.seek :garage @17:20:44.68 Hand.open @17:20:45.41 Forearm.reconfigure :loose @17:20:45.46 Forearm.seek :retract @17:20:46.40 da sampling at most 1000ml, wcr at most 1000ml @17:20:46.44 Loading sh1 puck from tube 3 into Collection Clamp @17:20:46.45 DAfans.email "Started da in tube 3 at 17:20:46UTC24-Oct-23\n{1 => 33,\n 2 => 11,\n 3 => 22,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@17:16:28, 27% flow, 17.9C, 21% humidity, 14.6psia, 13.720V, 0.272A, 214.037Ah, 3.74W",Subject: "da sampling at most 1000ml, wcr at most 1000ml" @17:20:46.72
SC.reconfigure :tight @17:20:46.79 SC.seek 3 @17:20:50.38 SC.configure :limp @17:20:50.43 upsync @17:20:50.65
Elbow.reconfigure :tight @17:20:50.70 Elbow.seek 3 @17:20:52.68 Forearm.reconfigure :tight @17:20:52.73 Forearm.seek 3 + 450 counts @17:20:53.71 SE.reconfigure :pick @17:20:53.77 SE.seek :beamBreak @17:20:55.53 SE.configure :default @17:20:55.69 Elbow.seek 3 @17:20:55.89 Forearm.seek 3 + 300 counts @17:20:56.15 Forearm.reconfigure :loose @17:20:56.20 Hand.close @17:20:56.87 SE.seek :down @17:20:57.07
Forearm.seek :retract @17:20:58.09 Elbow.seek CC @17:20:58.20 SE.stop @17:20:59.74
Elbow.reconfigure :loose @17:20:59.84 Forearm.reconfigure :tight @17:20:59.89 Forearm.seek CC @17:21:01.21 CC.seek :closed @17:21:05.75 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.27 @17:21:05.76 Hand.open @17:21:06.41 Forearm.reconfigure :loose @17:21:06.46 Forearm.seek :retract @17:21:07.48 collectSample 1000ml w/bubble point of 24psi @17:21:07.49 Priming 7ml @17:21:07.50 Intake.select :close @17:21:07.51 Exhaust.select :close @17:21:07.52 SS.hold @17:21:07.73 Calibrating pressure and temperature sensors @17:21:07.76 CTV.dial :air @17:21:07.78 SSV.dial :spit,avoiding: :exhaust @17:21:07.87 SS.reconfigure :fast @17:21:07.91 SS.seek 5ml @17:21:10.72 SSV.dial CBV @17:21:10.76
CBV.dial SSV @17:21:12.06 delay 3.8 seconds @17:21:15.99 Sensor limits: Intake max 110.0psia; Outlet max 102.3psia @17:21:16.00 SSV.dial :spit @17:21:17.33 SS.seek 0ml @17:21:20.14 SSV.dial CBV @17:21:21.44 CTV.dial :intake @17:21:22.23 SSV.dial CBV @17:21:22.25
CBV.dial SSV @17:21:22.40 Allowing 40s for 7.0ml of remaining 7.0ml @17:21:22.41 Intake.select :open @17:21:24.57 Intake @14.7psia, vacuum from 9.2 to 12.2psi @17:21:24.59 SS.reconfigure :inhaling 2.20ml/s @17:21:24.63 SS.seek 7ml @17:21:31.47 Stroke completed -- waiting for pressure to equalize ... @17:21:31.48 SS.reconfigure :exhale @17:21:31.52 SS.hold @17:21:36.71 Intake.select :close @17:21:36.86 SSV.dial :exhaust @17:21:37.61 Exhaust.select :open @17:21:37.72 SS.seek 0ml @17:21:42.09 Exhaust.select :close @17:21:42.20 Sampling 1000.0ml @17:21:42.21 Intake.select :close @17:21:42.22 Exhaust.select :close @17:21:42.23 SS.reconfigure :default @17:21:42.27 SS.hold @17:21:42.48 CTV.dial :intake @17:21:42.66 SSV.dial CBV @17:21:42.69
CBV.dial SSV @17:21:43.47 Allowing 130s for 25.0ml of remaining 1000.0ml @17:21:43.48 Intake.select :open @17:21:45.64 Intake @15.2psia, vacuum from 9.7 to 12.7psi @17:21:45.66 SS.reconfigure :inhaling 2.20ml/s @17:21:45.71 SS.seek 25ml @17:22:00.59 Stroke completed -- waiting for pressure to equalize ... @17:22:00.60 SS.reconfigure :exhale @17:22:00.65 SS.hold @17:22:15.09 Intake.select :close @17:22:15.24 SSV.dial :exhaust @17:22:16.00 Exhaust.select :open @17:22:16.11 SS.seek 0ml @17:22:24.92 Exhaust.select :close @17:22:25.03 SSV.dial CBV @17:22:25.07
CBV.dial SSV @17:22:25.81 Allowing 130s for 25.0ml of remaining 975.0ml @17:22:25.82 Intake.select :open @17:22:27.98 Intake @15.5psia, vacuum from 10.0 to 13.0psi @17:22:28.00 SS.reconfigure :inhaling 2.20ml/s @17:22:28.05 SS.seek 25ml @17:22:42.05 Vacuum too high -- waiting up to 40 seconds @17:22:42.07 SS.reconfigure :exhale @17:22:42.12 SS.hold @17:22:42.83 Intake @12.8psia, vacuum from 7.3 to 10.3psi @17:22:42.84 SS.reconfigure :inhaling 1.76ml/s @17:22:42.89 SS.seek 25ml @17:22:43.08 Vacuum too high -- waiting up to 40 seconds @17:22:43.10 SS.reconfigure :exhale @17:22:43.15 SS.hold @17:22:47.40 Intake @16.0psia, vacuum from 10.0 to 13.0psi @17:22:47.42 SS.reconfigure :inhaling 1.41ml/s @17:22:47.47 SS.seek 25ml @17:22:49.43 Stroke completed -- waiting for pressure to equalize ... @17:22:49.44 SS.reconfigure :exhale @17:22:49.49 SS.hold @17:22:59.27 Intake.select :close @17:22:59.42 SSV.dial :exhaust @17:23:00.19 Exhaust.select :open @17:23:00.30 SS.seek 0ml @17:23:09.18 Exhaust.select :close @17:23:09.29 SSV.dial CBV @17:23:09.32
CBV.dial SSV @17:23:10.09 Allowing 130s for 25.0ml of remaining 950.0ml @17:23:10.10 Intake.select :open @17:23:12.26 Intake @15.8psia, vacuum from 10.0 to 13.0psi @17:23:12.28 SS.reconfigure :inhaling 1.41ml/s @17:23:12.32 SS.seek 25ml @17:23:32.56 Stroke completed -- waiting for pressure to equalize ... @17:23:32.57 SS.reconfigure :exhale @17:23:32.61 SS.hold @17:23:42.79 Intake.select :close @17:23:42.94 SSV.dial :exhaust @17:23:43.70 Exhaust.select :open @17:23:43.81 SS.seek 0ml @17:23:52.56 Exhaust.select :close @17:23:52.67 SSV.dial CBV @17:23:52.71
CBV.dial SSV @17:23:53.45 Allowing 130s for 25.0ml of remaining 925.0ml @17:23:53.46 Intake.select :open @17:23:55.63 Intake @15.7psia, vacuum from 10.0 to 13.0psi @17:23:55.65 SS.reconfigure :inhaling 1.41ml/s @17:23:55.70 SS.seek 25ml @17:24:16.43 Stroke completed -- waiting for pressure to equalize ... @17:24:16.44 SS.reconfigure :exhale @17:24:16.49 SS.hold @17:24:26.41 Intake.select :close @17:24:26.56 SSV.dial :exhaust @17:24:27.33 Exhaust.select :open @17:24:27.44 SS.seek 0ml @17:24:36.38 Exhaust.select :close @17:24:36.50 SSV.dial CBV @17:24:36.52
CBV.dial SSV @17:24:37.29 Allowing 130s for 25.0ml of remaining 900.0ml @17:24:37.30 Intake.select :open @17:24:39.46 Intake @15.8psia, vacuum from 10.0 to 13.0psi @17:24:39.48 SS.reconfigure :inhaling 1.41ml/s @17:24:39.53 SS.seek 25ml @17:24:59.93 Stroke completed -- waiting for pressure to equalize ... @17:24:59.94 SS.reconfigure :exhale @17:24:59.99 SS.hold @17:25:08.95 Intake.select :close @17:25:09.11 SSV.dial :exhaust @17:25:09.87 Exhaust.select :open @17:25:09.98 SS.seek 0ml @17:25:18.60 Exhaust.select :close @17:25:18.71 SSV.dial CBV @17:25:18.74
CBV.dial SSV @17:25:19.51 Allowing 130s for 25.0ml of remaining 875.0ml @17:25:19.52 Intake.select :open @17:25:21.69 Intake @15.9psia, vacuum from 10.0 to 13.0psi @17:25:21.71 SS.reconfigure :inhaling 1.41ml/s @17:25:21.75 SS.seek 25ml @17:25:42.71 Stroke completed -- waiting for pressure to equalize ... @17:25:42.72 SS.reconfigure :exhale @17:25:42.77 SS.hold @17:25:50.23 Intake.select :close @17:25:50.38 SSV.dial :exhaust @17:25:51.15 Exhaust.select :open @17:25:51.26 SS.seek 0ml @17:26:00.07 Exhaust.select :close @17:26:00.18 SSV.dial CBV @17:26:00.20
CBV.dial SSV @17:26:01.01 Allowing 130s for 25.0ml of remaining 850.0ml @17:26:01.02 Intake.select :open @17:26:03.18 Intake @15.9psia, vacuum from 10.0 to 13.0psi @17:26:03.20 SS.reconfigure :inhaling 1.41ml/s @17:26:03.25 SS.seek 25ml @17:26:23.99 Stroke completed -- waiting for pressure to equalize ... @17:26:24.00 SS.reconfigure :exhale @17:26:24.05 SS.hold @17:26:32.58 Intake.select :close @17:26:32.73 SSV.dial :exhaust @17:26:33.49 Exhaust.select :open @17:26:33.60 SS.seek 0ml @17:26:42.40 Exhaust.select :close @17:26:42.51 SSV.dial CBV @17:26:42.53
CBV.dial SSV @17:26:43.32 Allowing 130s for 25.0ml of remaining 825.0ml @17:26:43.33 Intake.select :open @17:26:45.49 Intake @15.8psia, vacuum from 10.0 to 13.0psi @17:26:45.51 SS.reconfigure :inhaling 1.41ml/s @17:26:45.56 SS.seek 25ml @17:27:06.11 Stroke completed -- waiting for pressure to equalize ... @17:27:06.12 SS.reconfigure :exhale @17:27:06.17 SS.hold @17:27:13.84 Intake.select :close @17:27:13.99 SSV.dial :exhaust @17:27:14.76 Exhaust.select :open @17:27:14.87 SS.seek 0ml @17:27:23.63 Exhaust.select :close @17:27:23.74 SSV.dial CBV @17:27:23.76
CBV.dial SSV @17:27:24.54 Allowing 130s for 25.0ml of remaining 800.0ml @17:27:24.55 Intake.select :open @17:27:26.71 Intake @15.7psia, vacuum from 10.0 to 13.0psi @17:27:26.73 SS.reconfigure :inhaling 1.41ml/s @17:27:26.78 SS.seek 25ml @17:27:47.11 Stroke completed -- waiting for pressure to equalize ... @17:27:47.12 SS.reconfigure :exhale @17:27:47.17 SS.hold @17:27:54.97 Intake.select :close @17:27:55.13 SSV.dial :exhaust @17:27:55.90 Exhaust.select :open @17:27:56.01 SS.seek 0ml @17:28:04.72 Exhaust.select :close @17:28:04.84 SSV.dial CBV @17:28:04.86
CBV.dial SSV @17:28:05.65 Allowing 130s for 25.0ml of remaining 775.0ml @17:28:05.66 Intake.select :open @17:28:07.82 Intake @15.9psia, vacuum from 10.0 to 13.0psi @17:28:07.84 SS.reconfigure :inhaling 1.41ml/s @17:28:07.89 SS.seek 25ml @17:28:28.39 Stroke completed -- waiting for pressure to equalize ... @17:28:28.41 SS.reconfigure :exhale @17:28:28.45 SS.hold @17:28:35.91 Intake.select :close @17:28:36.06 SSV.dial :exhaust @17:28:36.82 Exhaust.select :open @17:28:36.93 SS.seek 0ml @17:28:45.58 Exhaust.select :close @17:28:45.69 SSV.dial CBV @17:28:45.72
CBV.dial SSV @17:28:46.49 Allowing 130s for 25.0ml of remaining 750.0ml @17:28:46.50 Intake.select :open @17:28:48.66 Intake @15.8psia, vacuum from 10.0 to 13.0psi @17:28:48.68 SS.reconfigure :inhaling 1.41ml/s @17:28:48.73 SS.seek 25ml @17:29:09.32 Stroke completed -- waiting for pressure to equalize ... @17:29:09.33 SS.reconfigure :exhale @17:29:09.37 SS.hold @17:29:18.17 Intake.select :close @17:29:18.33 SSV.dial :exhaust @17:29:19.10 Exhaust.select :open @17:29:19.22 SS.seek 0ml @17:29:28.04 Exhaust.select :close @17:29:28.15 SSV.dial CBV @17:29:28.17
CBV.dial SSV @17:29:28.96 Allowing 130s for 25.0ml of remaining 725.0ml @17:29:28.97 Intake.select :open @17:29:31.13 Intake @15.8psia, vacuum from 10.0 to 13.0psi @17:29:31.15 SS.reconfigure :inhaling 1.41ml/s @17:29:31.19 SS.seek 25ml @17:29:51.71 Stroke completed -- waiting for pressure to equalize ... @17:29:51.72 SS.reconfigure :exhale @17:29:51.76 SS.hold @17:30:01.52 Intake.select :close @17:30:01.68 SSV.dial :exhaust @17:30:02.45 Exhaust.select :open @17:30:02.56 SS.seek 0ml @17:30:11.53 Exhaust.select :close @17:30:11.65 SSV.dial CBV @17:30:11.67
CBV.dial SSV @17:30:12.46 Allowing 130s for 25.0ml of remaining 700.0ml @17:30:12.47 Intake.select :open @17:30:14.63 Intake @15.8psia, vacuum from 10.0 to 13.0psi @17:30:14.65 SS.reconfigure :inhaling 1.41ml/s @17:30:14.70 SS.seek 25ml @17:30:34.10 Vacuum too high -- waiting up to 40 seconds @17:30:34.12 SS.reconfigure :exhale @17:30:34.17 SS.hold @17:30:38.49 Intake @14.4psia, vacuum from 8.9 to 11.9psi @17:30:38.51 SS.reconfigure :inhaling 1.13ml/s @17:30:38.56 SS.seek 25ml @17:30:39.90 Stroke completed -- waiting for pressure to equalize ... @17:30:39.91 SS.reconfigure :exhale @17:30:39.95 SS.hold @17:30:45.58 Intake.select :close @17:30:45.74 SSV.dial :exhaust @17:30:46.50 Exhaust.select :open @17:30:46.62 SS.seek 0ml @17:30:55.58 Exhaust.select :close @17:30:55.69 SSV.dial CBV @17:30:55.73
CBV.dial SSV @17:30:56.46 Allowing 130s for 25.0ml of remaining 675.0ml @17:30:56.47 Intake.select :open @17:30:58.64 Intake @15.8psia, vacuum from 10.0 to 13.0psi @17:30:58.65 SS.reconfigure :inhaling 1.13ml/s @17:30:58.70 SS.seek 25ml @17:31:23.03 Stroke completed -- waiting for pressure to equalize ... @17:31:23.04 SS.reconfigure :exhale @17:31:23.09 SS.hold @17:31:32.76 Intake.select :close @17:31:32.91 SSV.dial :exhaust @17:31:33.68 Exhaust.select :open @17:31:33.79 SS.seek 0ml @17:31:42.53 Exhaust.select :close @17:31:42.64 SSV.dial CBV @17:31:42.67
CBV.dial SSV @17:31:43.44 Allowing 130s for 25.0ml of remaining 650.0ml @17:31:43.45 Intake.select :open @17:31:45.62 Intake @15.8psia, vacuum from 10.0 to 13.0psi @17:31:45.63 SS.reconfigure :inhaling 1.13ml/s @17:31:45.68 SS.seek 25ml @17:32:10.59 Stroke completed -- waiting for pressure to equalize ... @17:32:10.60 SS.reconfigure :exhale @17:32:11.01 SS.hold @17:32:22.21 Intake.select :close @17:32:22.36 SSV.dial :exhaust @17:32:23.13 Exhaust.select :open @17:32:23.24 SS.seek 0ml @17:32:32.00 Exhaust.select :close @17:32:32.11 SSV.dial CBV @17:32:32.13
CBV.dial SSV @17:32:32.97 Allowing 130s for 25.0ml of remaining 625.0ml @17:32:32.98 Intake.select :open @17:32:35.15 Intake @15.8psia, vacuum from 10.0 to 13.0psi @17:32:35.17 SS.reconfigure :inhaling 1.13ml/s @17:32:35.21 SS.seek 25ml @17:32:59.19 Vacuum too high -- waiting up to 40 seconds @17:32:59.21 SS.reconfigure :exhale @17:32:59.26 SS.hold @17:33:05.87 Intake @15.3psia, vacuum from 9.8 to 12.8psi @17:33:05.88 SS.reconfigure :inhaling 0.91ml/s @17:33:05.93 SS.seek 25ml @17:33:07.61 Stroke completed -- waiting for pressure to equalize ... @17:33:07.62 SS.reconfigure :exhale @17:33:07.66 SS.hold @17:33:14.15 Intake.select :close @17:33:14.30 SSV.dial :exhaust @17:33:15.07 Exhaust.select :open @17:33:15.18 SS.seek 0ml @17:33:24.23 Exhaust.select :close @17:33:24.34 SSV.dial CBV @17:33:24.37
CBV.dial SSV @17:33:25.14 Allowing 130s for 25.0ml of remaining 600.0ml @17:33:25.15 Intake.select :open @17:33:27.31 Intake @15.7psia, vacuum from 10.0 to 13.0psi @17:33:27.33 SS.reconfigure :inhaling 0.91ml/s @17:33:27.38 SS.seek 25ml @17:33:56.82 Stroke completed -- waiting for pressure to equalize ... @17:33:56.84 SS.reconfigure :exhale @17:33:56.88 SS.hold @17:34:08.14 Intake.select :close @17:34:08.30 SSV.dial :exhaust @17:34:09.06 Exhaust.select :open @17:34:09.18 SS.seek 0ml @17:34:18.12 Exhaust.select :close @17:34:18.23 SSV.dial CBV @17:34:18.26
CBV.dial SSV @17:34:19.02 Allowing 130s for 25.0ml of remaining 575.0ml @17:34:19.03 Intake.select :open @17:34:21.19 Intake @15.8psia, vacuum from 10.0 to 13.0psi @17:34:21.21 SS.reconfigure :inhaling 0.91ml/s @17:34:21.26 SS.seek 25ml @17:34:50.37 Stroke completed -- waiting for pressure to equalize ... @17:34:50.38 SS.reconfigure :exhale @17:34:50.43 SS.hold @17:35:04.90 Intake.select :close @17:35:05.05 SSV.dial :exhaust @17:35:05.81 Exhaust.select :open @17:35:05.92 SS.seek 0ml @17:35:14.67 Exhaust.select :close @17:35:14.78 SSV.dial CBV @17:35:14.82
CBV.dial SSV @17:35:15.56 Allowing 130s for 25.0ml of remaining 550.0ml @17:35:15.57 Intake.select :open @17:35:17.73 Intake @15.8psia, vacuum from 10.0 to 13.0psi @17:35:17.75 SS.reconfigure :inhaling 0.91ml/s @17:35:17.80 SS.seek 25ml @17:35:41.88 Vacuum too high -- waiting up to 40 seconds @17:35:41.89 SS.reconfigure :exhale @17:35:41.95 SS.hold @17:35:49.51 Intake @15.5psia, vacuum from 10.0 to 13.0psi @17:35:49.53 SS.reconfigure :inhaling 0.73ml/s @17:35:49.57 SS.seek 25ml @17:35:57.14 Stroke completed -- waiting for pressure to equalize ... @17:35:57.15 SS.reconfigure :exhale @17:35:57.19 SS.hold @17:36:06.02 Intake.select :close @17:36:06.17 SSV.dial :exhaust @17:36:06.93 Exhaust.select :open @17:36:07.05 SS.seek 0ml @17:36:16.07 Exhaust.select :close @17:36:16.18 SSV.dial CBV @17:36:16.21
CBV.dial SSV @17:36:17.00 Allowing 130s for 25.0ml of remaining 525.0ml @17:36:17.01 Intake.select :open @17:36:19.17 Intake @15.7psia, vacuum from 10.0 to 13.0psi @17:36:19.19 SS.reconfigure :inhaling 0.73ml/s @17:36:19.24 SS.seek 25ml @17:36:55.23 Stroke completed -- waiting for pressure to equalize ... @17:36:55.24 SS.reconfigure :exhale @17:36:55.33 SS.hold @17:37:12.08 Intake.select :close @17:37:12.24 SSV.dial :exhaust @17:37:13.00 Exhaust.select :open @17:37:13.11 SS.seek 0ml @17:37:21.87 Exhaust.select :close @17:37:21.98 SSV.dial CBV @17:37:22.01
CBV.dial SSV @17:37:22.78 Allowing 130s for 25.0ml of remaining 500.0ml @17:37:22.79 Intake.select :open @17:37:24.95 Intake @15.8psia, vacuum from 10.0 to 13.0psi @17:37:24.97 SS.reconfigure :inhaling 0.73ml/s @17:37:25.02 SS.seek 25ml @17:37:57.63 Vacuum too high -- waiting up to 40 seconds @17:37:57.64 SS.reconfigure :exhale @17:37:57.69 SS.hold @17:38:06.82 Intake @16.1psia, vacuum from 10.0 to 13.0psi @17:38:06.83 SS.reconfigure :inhaling 0.59ml/s @17:38:06.88 SS.seek 25ml @17:38:11.59 Stroke completed -- waiting for pressure to equalize ... @17:38:11.60 SS.reconfigure :exhale @17:38:11.64 SS.hold @17:38:22.81 Intake.select :close @17:38:22.96 SSV.dial :exhaust @17:38:23.73 Exhaust.select :open @17:38:23.84 SS.seek 0ml @17:38:32.85 Exhaust.select :close @17:38:32.96 SSV.dial CBV @17:38:33.00
CBV.dial SSV @17:38:33.76 Allowing 130s for 25.0ml of remaining 475.0ml @17:38:33.77 Intake.select :open @17:38:35.94 Intake @15.7psia, vacuum from 10.0 to 13.0psi @17:38:35.95 SS.reconfigure :inhaling 0.59ml/s @17:38:36.00 SS.seek 25ml @17:39:20.22 Stroke completed -- waiting for pressure to equalize ... @17:39:20.24 SS.reconfigure :exhale @17:39:20.28 SS.hold @17:39:38.76 Intake.select :close @17:39:38.92 SSV.dial :exhaust @17:39:39.68 Exhaust.select :open @17:39:39.79 SS.seek 0ml @17:39:48.59 Exhaust.select :close @17:39:48.70 SSV.dial CBV @17:39:48.72
CBV.dial SSV @17:39:49.52 Allowing 130s for 25.0ml of remaining 450.0ml @17:39:49.53 Intake.select :open @17:39:51.70 Intake @15.8psia, vacuum from 10.0 to 13.0psi @17:39:51.72 SS.reconfigure :inhaling 0.59ml/s @17:39:51.77 SS.seek 25ml @17:40:36.72 Vacuum too high -- waiting up to 40 seconds @17:40:36.73 SS.reconfigure :exhale @17:40:36.78 SS.hold @17:40:45.52 Intake @14.9psia, vacuum from 9.4 to 12.4psi @17:40:45.53 SS.reconfigure :inhaling 0.47ml/s @17:40:45.58 SS.seek 25ml @17:40:45.65 Stroke completed -- waiting for pressure to equalize ... @17:40:45.66 SS.reconfigure :exhale @17:40:45.70 SS.hold @17:40:58.37 Intake.select :close @17:40:58.52 SSV.dial :exhaust @17:40:59.29 Exhaust.select :open @17:40:59.40 SS.seek 0ml @17:41:08.41 Exhaust.select :close @17:41:08.52 SSV.dial CBV @17:41:08.54
CBV.dial SSV @17:41:09.73 Allowing 130s for 25.0ml of remaining 425.0ml @17:41:09.74 Intake.select :open @17:41:11.90 Intake @15.7psia, vacuum from 10.0 to 13.0psi @17:41:11.92 SS.reconfigure :inhaling 0.47ml/s @17:41:11.97 SS.seek 25ml @17:42:07.11 Stroke completed -- waiting for pressure to equalize ... @17:42:07.13 SS.reconfigure :exhale @17:42:07.17 SS.hold @17:42:27.56 Intake.select :close @17:42:27.71 SSV.dial :exhaust @17:42:28.48 Exhaust.select :open @17:42:28.60 SS.seek 0ml @17:42:37.55 Exhaust.select :close @17:42:37.66 SSV.dial CBV @17:42:37.68
CBV.dial SSV @17:42:38.46 Allowing 130s for 25.0ml of remaining 400.0ml @17:42:38.47 Intake.select :open @17:42:40.63 Intake @15.8psia, vacuum from 10.0 to 13.0psi @17:42:41.00 SS.reconfigure :inhaling 0.47ml/s @17:42:41.12 SS.seek 25ml @17:43:36.25 Stroke completed -- waiting for pressure to equalize ... @17:43:36.26 SS.reconfigure :exhale @17:43:36.31 SS.hold @17:44:00.72 Intake.select :close @17:44:00.87 SSV.dial :exhaust @17:44:01.63 Exhaust.select :open @17:44:01.74 SS.seek 0ml @17:44:10.55 Exhaust.select :close @17:44:10.66 SSV.dial CBV @17:44:10.69
CBV.dial SSV @17:44:11.46 Allowing 130s for 25.0ml of remaining 375.0ml @17:44:11.47 Intake.select :open @17:44:13.63 Intake @15.9psia, vacuum from 10.0 to 13.0psi @17:44:13.65 SS.reconfigure :inhaling 0.47ml/s @17:44:14.12 SS.seek 25ml @17:44:56.99 Vacuum too high -- waiting up to 40 seconds @17:44:57.01 SS.reconfigure :exhale @17:44:57.06 SS.hold @17:45:08.31 Intake @16.1psia, vacuum from 10.0 to 13.0psi @17:45:08.33 SS.reconfigure :inhaling 0.38ml/s @17:45:08.38 SS.seek 25ml @17:45:24.33 Stroke completed -- waiting for pressure to equalize ... @17:45:24.34 SS.reconfigure :exhale @17:45:24.38 SS.hold @17:45:44.85 Intake.select :close @17:45:45.29 SSV.dial :exhaust @17:45:46.06 Exhaust.select :open @17:45:46.18 SS.seek 0ml @17:45:54.94 Exhaust.select :close @17:45:55.05 SSV.dial CBV @17:45:55.08
CBV.dial SSV @17:45:55.83 Allowing 130s for 25.0ml of remaining 350.0ml @17:45:55.84 Intake.select :open @17:45:58.00 Intake @15.8psia, vacuum from 10.0 to 13.0psi @17:45:58.02 SS.reconfigure :inhaling 0.38ml/s @17:45:58.07 SS.seek 25ml @17:47:07.06 Stroke completed -- waiting for pressure to equalize ... @17:47:07.07 SS.reconfigure :exhale @17:47:07.12 SS.hold @17:47:36.12 Intake.select :close @17:47:36.27 SSV.dial :exhaust @17:47:37.05 Exhaust.select :open @17:47:37.16 SS.seek 0ml @17:47:45.98 Exhaust.select :close @17:47:46.09 SSV.dial CBV @17:47:46.11
CBV.dial SSV @17:47:46.92 Allowing 130s for 25.0ml of remaining 325.0ml @17:47:46.93 Intake.select :open @17:47:49.09 Intake @15.9psia, vacuum from 10.0 to 13.0psi @17:47:49.11 SS.reconfigure :inhaling 0.38ml/s @17:47:49.16 SS.seek 25ml @17:48:49.02 Vacuum too high -- waiting up to 40 seconds @17:48:49.03 SS.reconfigure :exhale @17:48:49.08 SS.hold @17:49:01.95 SS.reconfigure :inhaling 0.31ml/s @17:49:02.00 SS.seek 25ml @17:49:14.87 Stroke completed -- waiting for pressure to equalize ... @17:49:14.88 SS.reconfigure :exhale @17:49:14.93 SS.hold @17:49:35.31 Intake.select :close @17:49:35.46 SSV.dial :exhaust @17:49:36.24 Exhaust.select :open @17:49:36.78 SS.seek 0ml @17:49:45.82 Exhaust.select :close @17:49:45.94 SSV.dial CBV @17:49:45.96
CBV.dial SSV @17:49:46.73 Allowing 130s for 25.0ml of remaining 300.0ml @17:49:46.74 Intake.select :open @17:49:48.90 Intake @15.8psia, vacuum from 10.0 to 13.0psi @17:49:48.92 SS.reconfigure :inhaling 0.31ml/s @17:49:48.97 SS.seek 25ml @17:51:16.76 Stroke completed -- waiting for pressure to equalize ... @17:51:16.77 SS.reconfigure :exhale @17:51:16.81 SS.hold @17:51:47.86 Intake.select :close @17:51:48.02 SSV.dial :exhaust @17:51:48.79 Exhaust.select :open @17:51:48.90 SS.seek 0ml @17:51:57.95 Exhaust.select :close @17:51:58.06 SSV.dial CBV @17:51:58.08
CBV.dial SSV @17:51:58.87 Allowing 130s for 25.0ml of remaining 275.0ml @17:51:58.88 Intake.select :open @17:52:01.05 Intake @15.9psia, vacuum from 10.0 to 13.0psi @17:52:01.06 SS.reconfigure :inhaling 0.31ml/s @17:52:01.11 SS.seek 25ml @17:53:29.43 Stroke completed -- waiting for pressure to equalize ... @17:53:29.44 SS.reconfigure :exhale @17:53:29.48 SS.hold @17:54:06.29 Intake.select :close @17:54:06.44 SSV.dial :exhaust @17:54:07.21 Exhaust.select :open @17:54:07.32 SS.seek 0ml @17:54:16.38 Exhaust.select :close @17:54:16.49 SSV.dial CBV @17:54:16.51
CBV.dial SSV @17:54:17.27 Allowing 130s for 25.0ml of remaining 250.0ml @17:54:17.28 Intake.select :open @17:54:19.44 Intake @16.0psia, vacuum from 10.0 to 13.0psi @17:54:19.46 SS.reconfigure :inhaling 0.31ml/s @17:54:19.51 SS.seek 25ml @17:55:40.20 Vacuum too high -- waiting up to 40 seconds @17:55:40.21 SS.reconfigure :exhale @17:55:40.26 SS.hold @17:55:58.89 Intake @16.9psia, vacuum from 10.0 to 13.0psi @17:55:58.90 SS.reconfigure :inhaling 0.25ml/s @17:55:58.95 SS.seek 25ml @17:56:10.53 Stroke completed -- waiting for pressure to equalize ... @17:56:10.54 SS.reconfigure :exhale @17:56:10.59 SS.hold @17:56:27.29 SS.reconfigure :default @17:56:27.34 SS.hold @17:56:27.40 Intake.select :close @17:56:27.56 ShallowSampler::Clogged in MAIN -- Filtering too Slowly after 775.0ml @17:56:27.57 SSV.dial :exhaust @17:56:28.33 Exhaust.select :open @17:56:28.44 SS.reconfigure :exhale @17:56:28.49 SS.seek 0ml @17:56:37.47 Exhaust.select :close @17:56:37.58 Sampled 775.0ml @17:56:37.59 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @17:56:37.60 Intake.select :close @17:56:37.67 CSV.dial :waste @17:56:38.27 SSV.dial CBV,avoiding: :exhaust @17:56:38.30 CS.seek 0ml @17:56:38.33
CBV.dial SSV @17:56:38.49 CSV.dial :air @17:56:39.23 CS.seek 10ml @17:56:40.21
CTV.dial :air @17:56:40.99 delay 15 seconds @17:56:56.01 SSV.dial :spit,avoiding: :exhaust @17:56:57.32 CTV.dial CSV,avoiding: :intake @17:56:57.36
SS.seek 0ml @17:56:57.49 SSV.dial CBV,avoiding: :exhaust @17:57:04.05 SSV.dial between CBV and plug @17:57:04.54 CBV.dial between SSV and waste2 @17:57:04.92 CSV.dial between air and CTV @17:57:05.35 CS.seek 8.301ml @17:57:10.06 CTV.dial CSV,avoiding: :air @17:57:10.09
CSV.dial CTV @17:57:10.61 CS.reconfigure :pressurize @17:57:10.67 CS.seek 0ml @17:57:25.65 BubblePoint: Intake @24.3psia, Outlet @0.9psia @17:57:25.66 CBV.dial SSV @17:57:25.99 SS.reconfigure :default @17:57:26.04 SS.seek 10ml @17:57:31.53 delay 15 seconds @17:57:46.66 Evacuated: Intake @23.8psia, Outlet @23.3psia @17:57:46.67 SSV.dial :spit,avoiding: :exhaust @17:57:47.71 SS.reconfigure :exhale @17:57:47.76 SS.seek 0ml @17:57:52.95 SS.reconfigure :fast @17:57:53.00 SS.seek 5ml @17:57:55.85 SSV.dial CBV,avoiding: :exhaust @17:57:55.89
CBV.dial SSV @17:57:57.19 CTV.dial :air,avoiding: :intake @17:57:58.50 CSV.dial CTV @17:57:58.54
SS.seek 10ml @17:57:59.66 CSV.dial :air @17:58:00.11 CS.reconfigure :fast @17:58:00.16 CS.seek 0ml @17:58:01.21
SSV.dial :spit,avoiding: :exhaust @17:58:02.52 SS.reconfigure :exhale @17:58:02.57 SS.seek 0ml @17:58:07.01 CS.stop @17:58:07.67
SS.reconfigure :fast @17:58:08.14 SS.seek 5ml @17:58:11.05 SSV.dial CBV,avoiding: :exhaust @17:58:11.08
CBV.dial SSV @17:58:13.40 SSV.dial :spit,avoiding: :exhaust @17:58:14.74 SS.reconfigure :default @17:58:14.79 SS.seek 0ml @17:58:17.91 SS.stop @17:58:17.95 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @17:58:17.96 Intake.select :close @17:58:18.03 CSV.dial :waste @17:58:18.16 SSV.dial CBV,avoiding: :exhaust @17:58:18.19
CBV.dial SSV @17:58:18.80 CS.seek 0ml @17:58:18.87 CSV.dial :air @17:58:19.52
CTV.dial :air @17:58:19.61 CS.seek 10ml @17:58:19.66
delay 15 seconds @17:58:34.69 SSV.dial :spit,avoiding: :exhaust @17:58:36.01 CTV.dial CSV,avoiding: :intake @17:58:36.03
SS.reconfigure :exhale @17:58:36.14 SS.seek 0ml @17:58:36.52 SSV.dial CBV,avoiding: :exhaust @17:58:44.27 SSV.dial between CBV and plug @17:58:44.75 CBV.dial between SSV and waste2 @17:58:45.13 CSV.dial between air and CTV @17:58:45.44 CS.seek 6.944ml @17:58:53.27 CTV.dial CSV,avoiding: :air @17:58:53.30
CSV.dial CTV @17:58:53.75 CS.reconfigure :pressurize @17:58:53.80 CS.seek 0ml @17:59:07.43 BubblePoint: Intake @24.3psia, Outlet @4.5psia @17:59:07.44 CBV.dial SSV @17:59:07.77 SS.reconfigure :default @17:59:07.82 SS.seek 10ml @17:59:13.38 delay 15 seconds @17:59:28.51 Evacuated: Intake @24.0psia, Outlet @23.3psia @17:59:28.52 SSV.dial :spit,avoiding: :exhaust @17:59:29.55 SS.reconfigure :exhale @17:59:29.60 SS.seek 0ml @17:59:34.62 SS.reconfigure :fast @17:59:34.67 SS.seek 5ml @17:59:37.47 SSV.dial CBV,avoiding: :exhaust @17:59:37.50
CBV.dial SSV @17:59:38.85 CTV.dial :air,avoiding: :intake @17:59:40.20 CSV.dial CTV @17:59:40.24
SS.seek 10ml @17:59:41.80 CSV.dial :air @17:59:42.24 CS.reconfigure :fast @17:59:42.29 CS.seek 0ml @17:59:43.21
SSV.dial :spit,avoiding: :exhaust @17:59:44.52 SS.reconfigure :exhale @17:59:44.57 SS.seek 0ml @17:59:47.26 CS.stop @17:59:49.56
SS.reconfigure :fast @17:59:49.60 SS.seek 5ml @17:59:52.37 SSV.dial CBV,avoiding: :exhaust @17:59:52.40
CBV.dial SSV @17:59:54.76 SSV.dial :spit,avoiding: :exhaust @17:59:56.08 SS.reconfigure :default @17:59:56.13 SS.seek 0ml @17:59:59.36 SS.stop @17:59:59.40 Lysing: Adding 2.0ml AqMeOH @17:59:59.41 CBV.dial between CSV and waste1,avoiding: :DBV @18:00:00.89 CTV.dial CSV @18:00:02.20 CSV.dial CSR @18:00:02.99 Pulling 1ml of CSR.AqMeOH @18:00:03.00 CSR.select :AqMeOH @18:00:03.12 CS.reconfigure :default @18:00:03.16 CS.seek 1ml @18:00:06.54 delay 5 seconds @18:00:11.56 CSR.select :air @18:00:11.73 CS.seek 6ml @18:00:25.39 delay 2 seconds @18:00:27.40 CSV.dial :waste2 @18:00:27.85 CS.reconfigure :fast @18:00:27.89 CS.seek 0ml @18:00:42.42 CSV.dial CSR @18:00:42.87 Pulling 2ml of CSR.AqMeOH @18:00:42.88 CSR.select :AqMeOH @18:00:43.00 CS.reconfigure :default @18:00:43.04 CS.seek 2ml @18:00:49.01 delay 5 seconds @18:00:54.03 CSR.select :air @18:00:54.19 CS.seek 5ml @18:01:02.82 delay 2 seconds @18:01:04.84 CSV.dial CTV @18:01:05.28 CS.seek 1ml @18:01:16.46 Before lysing Collection Puck Heater at 16.3C/38.7psia @18:01:16.48 Heating Puck.from 3, :sh1 in Collection Clamp to 70C for 10:00 @18:01:16.50 CH.seek 70C,8:00 @18:03:41.27 CH.hold 10:00,70C @18:03:41.46 Begun lysing Collection Puck Heater at 68.1C/45.2psia @18:13:41.47 Ended lysing Collection Puck Heater at 70C/44.3psia @18:13:41.48 CS.seek 0ml @18:13:44.88 CTV.dial between spare and air,avoiding: :intake @18:13:45.92 CSV.dial :air @18:13:46.37 CS.seek 5ml @18:13:59.97 CSV.dial CTV @18:14:00.42 delay 2 seconds @18:14:02.44 CSV.dial :air @18:14:02.89 delay 2 seconds @18:14:04.91 CSV.dial CTV @18:14:05.36 delay 2 seconds @18:14:07.38 CSV.dial :air @18:14:07.83 delay 2 seconds @18:14:09.85 CS.seek 0ml @18:14:23.46 Puck @95.5psia after final lysis pressurization @18:14:23.47 Recovering Lysate into Collection syringe @18:14:23.48 CSV.dial CBV @18:14:24.50 CBV.dial CSV @18:14:24.89 CS.seek 5ml @18:14:38.53 delay 5 seconds @18:14:43.55 CTV.dial :air @18:14:44.09 CS.seek 7ml @18:14:50.03 delay 5 seconds @18:14:55.05 CSV.dial :air2 @18:14:55.49 CS.seek 10ml @18:15:04.16 CSV.dial between air and CTV,avoiding: CBV @18:15:05.31 Lysing Complete @18:15:05.38 Storing sh1 puck in Collection Clamp to tube 2 @18:15:05.56 SC.reconfigure :tight @18:15:05.61 SC.seek 2 @18:15:09.10 SC.configure :limp @18:15:09.76 Elbow.reconfigure :tight @18:15:09.81 Elbow.seek 2 @18:15:11.60 Forearm.reconfigure :tight @18:15:11.65 Forearm.seek 2 + 450 counts @18:15:12.64 SE.reconfigure :pick @18:15:12.69 SE.seek :beamBreak @18:15:15.12 SE.configure :default @18:15:15.28 Elbow.reconfigure :limp @18:15:15.33 Forearm.reconfigure :loose @18:15:15.38 Forearm.seek 2 @18:15:15.60 Elbow.hold @18:15:15.67 Elbow.reconfigure :tight @18:15:15.77 SE.seek 10.12 Pucks below Top Plate @18:15:16.23 Forearm.force -20 @18:15:16.27 Forearm.seek 2 + 300 counts @18:15:16.57 Forearm.reconfigure :tight @18:15:16.61 Forearm.seek 2 + 450 counts @18:15:16.83 SE.seek 11.12 Pucks below Top Plate @18:15:17.37 Forearm.reconfigure :loose @18:15:17.42 Forearm.seek :retract @18:15:18.33 Elbow.seek CC @18:15:20.02 Elbow.reconfigure :loose @18:15:20.13 Forearm.seek CC @18:15:21.09 Hand.close @18:15:21.68 CC.seek :open @18:15:26.42 Forearm.seek :retract @18:15:27.40 Elbow.reconfigure :tight @18:15:27.45 Elbow.seek 2 @18:15:29.22 Elbow.reconfigure :limp @18:15:29.27 Forearm.seek 2 @18:15:30.73 Elbow.hold @18:15:30.79 Elbow.reconfigure :tight @18:15:30.84 Hand.open @18:15:31.55 Forearm.force -20 @18:15:31.59 Forearm.seek 2 + 304 counts @18:15:31.90 Forearm.reconfigure :tight @18:15:31.94 Forearm.seek 2 + 450 counts @18:15:32.21 SE.seek :down @18:15:33.35
Forearm.reconfigure :loose @18:15:33.39 Forearm.seek :retract @18:15:34.16 SE.stop @18:15:34.31
Tranferring DA @18:15:34.32 Beginning samplePassC @18:15:34.33 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @18:15:34.53 Elbow.seek FlushPuck::Garage @18:15:36.27 Elbow.reconfigure :loose @18:15:36.37 Forearm.seek :garage @18:15:37.38 Hand.close @18:15:38.18 Forearm.seek :retract @18:15:39.15 Elbow.reconfigure :tight @18:15:39.20 Elbow.seek CC @18:15:42.11 Elbow.reconfigure :loose @18:15:42.21 Forearm.reconfigure :tight @18:15:42.26 Forearm.seek CC @18:15:43.51 CC.seek :closed @18:15:48.03 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.31 @18:15:48.09 Hand.open @18:15:48.75 Forearm.reconfigure :loose @18:15:48.79 Forearm.seek :retract @18:15:49.81 PSV.dial PSR @18:15:51.31 Pulling 1.5ml of PSR.flush @18:15:51.32 PSR.select :flush @18:15:51.44 PS.seek 1.5ml @18:16:07.56 delay 5 seconds @18:16:12.58 PSV.dial :waste1 @18:16:13.07 PS.seek 0ml @18:16:29.19 PSV.dial PSR @18:16:29.66 PSR.select :air @18:16:29.78 PS.seek 2.5ml @18:16:56.22 PRV.dial CBV,avoiding: :ab2 @18:16:56.25
CBV.dial PRV @18:16:58.00 PSV.dial :mixloop @18:16:59.03 CTV.dial :air,avoiding: :intake @18:16:59.21 PTV.dial :PRVmixing @18:17:00.55 PS.seek 0ml @18:17:26.97 PSV.dial :air @18:17:27.55 PS.seek 2.5ml @18:17:54.06 PSV.dial :mixloop @18:17:54.52 PS.seek 0ml @18:18:20.87 PSV.dial :air @18:18:21.35 PS.seek 2.5ml @18:18:47.75 PSV.dial :mixloop @18:18:48.22 PS.seek 0ml @18:19:14.56 Garaging FlushPuck in Collection Clamp @18:19:14.63 Elbow.reconfigure :tight @18:19:14.68 Elbow.seek CC @18:19:15.15 Elbow.reconfigure :loose @18:19:15.25 Forearm.seek CC @18:19:16.24 Hand.close @18:19:16.81 CC.seek :open @18:19:21.53 Forearm.seek :retract @18:19:22.49 Elbow.reconfigure :tight @18:19:22.54 Elbow.seek :garage @18:19:25.50 Elbow.reconfigure :loose @18:19:25.60 Forearm.reconfigure :tight @18:19:25.65 Forearm.seek :garage @18:19:26.69 Hand.open @18:19:27.37 Forearm.reconfigure :loose @18:19:27.41 Forearm.seek :retract @18:19:28.38 PSV.dial :mixloop @18:19:28.45 PTV.dial :PRVmixing @18:19:28.53 PRV.dial CBV @18:19:28.56
CBV.dial PRV @18:19:28.75 CTV.dial :air,avoiding: :intake @18:19:28.77 Loading lyfil puck from tube 3 into Collection Clamp @18:19:29.00 SC.reconfigure :tight @18:19:29.05 SC.seek 3 @18:19:32.56 SC.configure :limp @18:19:32.78 Elbow.reconfigure :tight @18:19:32.82 Elbow.seek 3 @18:19:34.74 Forearm.reconfigure :tight @18:19:34.79 Forearm.seek 3 + 450 counts @18:19:35.85 SE.reconfigure :pick @18:19:35.91 SE.seek :beamBreak @18:19:37.75 SE.configure :default @18:19:37.91 Elbow.seek 3 @18:19:38.10 Forearm.seek 3 + 300 counts @18:19:38.35 Forearm.reconfigure :loose @18:19:38.40 Hand.close @18:19:39.13 SE.seek :down @18:19:39.34
Forearm.seek :retract @18:19:40.32 Elbow.seek CC @18:19:40.56 SE.stop @18:19:41.95
Elbow.reconfigure :loose @18:19:42.06 Forearm.reconfigure :tight @18:19:42.11 Forearm.seek CC @18:19:43.35 CC.seek :closed @18:19:47.88 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.11 @18:19:47.89 Hand.open @18:19:48.62 Forearm.reconfigure :loose @18:19:48.66 Forearm.seek :retract @18:19:49.62 CTV.dial CSV,avoiding: :intake @18:19:50.96 CSV.dial CTV @18:19:51.30 PS.seek 2.5ml @18:20:17.68 CS.seek 4.5ml @18:20:32.59 delay 8 seconds @18:20:40.61 PSV.dial :air @18:20:41.08 delay 2 seconds @18:20:43.09 PSV.dial :waste2 @18:20:43.83 PS.seek 0ml @18:21:10.21 CBV.dial between PRV and waste1 @18:21:10.54 CSV.dial :air @18:21:11.00 CS.seek 10ml @18:21:25.96 CSV.dial CTV @18:21:26.42 CS.seek 0ml @18:21:53.21 CBV.dial :waste1 @18:21:53.57 delay 5 seconds @18:21:58.59 CTV.dial :air,avoiding: :intake @18:22:00.46 Storing lyfil puck in Collection Clamp to tube 2 @18:22:00.64 SC.reconfigure :tight @18:22:00.69 SC.seek 2 @18:22:04.28 SC.configure :limp @18:22:04.50 Elbow.reconfigure :tight @18:22:04.55 Elbow.seek 2 @18:22:06.33 Forearm.reconfigure :tight @18:22:06.37 Forearm.seek 2 + 450 counts @18:22:07.44 SE.reconfigure :pick @18:22:07.50 SE.seek :beamBreak @18:22:09.87 SE.configure :default @18:22:10.03 Elbow.reconfigure :limp @18:22:10.08 Forearm.reconfigure :loose @18:22:10.12 Forearm.seek 2 @18:22:10.28 Elbow.hold @18:22:10.35 Elbow.reconfigure :tight @18:22:10.45 SE.seek 11.1 Pucks below Top Plate @18:22:10.91 Forearm.force -20 @18:22:10.95 Forearm.seek 2 + 315 counts @18:22:11.25 Forearm.reconfigure :tight @18:22:11.30 Forearm.seek 2 + 450 counts @18:22:11.53 SE.seek 12.1 Pucks below Top Plate @18:22:12.06 Forearm.reconfigure :loose @18:22:12.10 Forearm.seek :retract @18:22:13.08 Elbow.seek CC @18:22:14.76 Elbow.reconfigure :loose @18:22:14.87 Forearm.seek CC @18:22:16.28 Hand.close @18:22:16.93 CC.seek :open @18:22:21.67 Forearm.seek :retract @18:22:22.62 Elbow.reconfigure :tight @18:22:22.66 Elbow.seek 2 @18:22:24.42 Elbow.reconfigure :limp @18:22:24.47 Forearm.seek 2 @18:22:25.47 Elbow.hold @18:22:25.54 Elbow.reconfigure :tight @18:22:25.59 Hand.open @18:22:26.30 Forearm.force -20 @18:22:26.34 Forearm.seek 2 + 308 counts @18:22:26.74 Forearm.reconfigure :tight @18:22:26.78 Forearm.seek 2 + 450 counts @18:22:27.12 SE.seek :down @18:22:28.26
Forearm.reconfigure :loose @18:22:28.30 Forearm.seek :retract @18:22:29.01 SE.stop @18:22:29.26
PSV.dial :air @18:22:30.02 PS.seek 2.5ml @18:22:56.40 delay 1 second @18:22:57.41 PSV.dial :mixloop @18:22:57.87 delay 1 second @18:22:58.89 PSV.dial :air @18:22:59.35 delay 1 second @18:23:00.37 PSV.dial :mixloop @18:23:00.84 delay 1 second @18:23:01.85 PSV.dial :air @18:23:02.32 delay 1 second @18:23:03.34 PS.seek 0ml @18:23:29.71 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @18:23:30.34 Elbow.seek FlushPuck::Garage @18:23:32.15 Elbow.reconfigure :loose @18:23:32.26 Forearm.seek :garage @18:23:33.27 Hand.close @18:23:34.04 Forearm.seek :retract @18:23:35.01 Elbow.reconfigure :tight @18:23:35.06 Elbow.seek PC @18:23:36.35 Elbow.reconfigure :loose @18:23:36.46 Forearm.reconfigure :tight @18:23:36.50 Forearm.seek PC @18:23:37.70 PC.seek :closed @18:23:42.36 Processing Clamp onSpring/offSpring for 16/26 samples, on/off torque 1.63 @18:23:42.43 Hand.open @18:23:43.11 Forearm.reconfigure :loose @18:23:43.15 Forearm.seek :retract @18:23:44.13 PRV.dial :air @18:23:44.59 PSV.dial :mixloop @18:23:45.12 PS.seek 0.3ml @18:23:49.02 PSV.dial PSR @18:23:50.08 Pulling 0.4ml of PSR.air @18:23:50.09 PSR.select :air @18:23:50.11 PS.seek 0.7ml @18:23:54.99 delay 3 seconds @18:23:58.01 PSR.select :air @18:23:58.03 PS.seek 2.5ml @18:24:17.27 delay 3 seconds @18:24:20.29 PSV.dial :mixloop @18:24:21.34 PTV.dial :mixing @18:24:22.10 PBV.dial :waste2 @18:24:23.50 PS.seek 0ml @18:24:49.84 Garaging FlushPuck in Processing Clamp @18:24:49.91 Elbow.reconfigure :tight @18:24:49.96 Elbow.seek PC @18:24:50.08 Elbow.reconfigure :loose @18:24:50.18 Forearm.seek PC @18:24:51.17 Hand.close @18:24:51.80 PC.seek :open @18:24:56.52 Forearm.seek :retract @18:24:57.49 Elbow.reconfigure :tight @18:24:57.54 Elbow.seek :garage @18:24:58.92 Elbow.reconfigure :loose @18:24:59.02 Forearm.reconfigure :tight @18:24:59.07 Forearm.seek :garage @18:25:00.14 Hand.open @18:25:00.86 Forearm.reconfigure :loose @18:25:00.91 Forearm.seek :retract @18:25:01.90 Loading da puck from tube 3 into Processing Clamp @18:25:02.13 SC.reconfigure :tight @18:25:02.18 SC.seek 3 @18:25:05.70 SC.configure :limp @18:25:05.91 Elbow.reconfigure :tight @18:25:05.96 Elbow.seek 3 @18:25:07.84 Forearm.reconfigure :tight @18:25:07.89 Forearm.seek 3 + 450 counts @18:25:08.92 SE.reconfigure :pick @18:25:08.98 SE.seek :beamBreak @18:25:10.83 SE.configure :default @18:25:10.99 Elbow.seek 3 @18:25:11.86 Forearm.seek 3 + 300 counts @18:25:12.11 Forearm.reconfigure :loose @18:25:12.16 Hand.close @18:25:12.82 SE.seek :down @18:25:13.03
Forearm.seek :retract @18:25:13.96 Elbow.seek PC @18:25:14.31 SE.stop @18:25:16.45
Elbow.reconfigure :loose @18:25:16.55 Forearm.reconfigure :tight @18:25:16.60 Forearm.seek PC @18:25:17.90 PC.seek :closed @18:25:22.61 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.65 @18:25:22.62 Hand.open @18:25:23.24 Forearm.reconfigure :loose @18:25:23.28 Forearm.seek :retract @18:25:24.21 Heating Puck.from 3, :da in Processing Clamp to 28C for 2:00:00 @18:25:24.23 PH.seek 28C,5:00 @18:26:03.78 PH.hold 2:00:00,28C @18:26:03.83 delay 1:30 @18:27:33.88 PSR.select :air @18:27:33.90 PSV.dial PSR @18:27:34.94 Pulling 0.5ml of PSR.wash @18:27:34.95 PSR.select :wash @18:27:35.07 PS.seek 0.5ml @18:27:41.05 delay 5 seconds @18:27:46.07 PSR.select :air @18:27:46.19 PS.seek 2ml @18:28:02.55 PTV.dial :puck @18:28:03.28 PSV.dial PTV @18:28:04.05 PBV.dial :waste1 @18:28:05.44 PS.seek 1.5ml @18:28:11.49 delay 3 seconds @18:28:14.51 PBV.dial between PSV and waste1 @18:28:14.87 PS.seek 0ml @18:28:31.02 delay 5:00 @18:33:31.04 recoverPSRreagent08 @18:33:31.05 PTV.dial :puck @18:33:31.07 PBV.dial :waste1 @18:33:31.44 delay 30 seconds @18:34:01.45 PBV.dial between PSV and waste1 @18:34:01.81 PSR.select :air @18:34:01.83 PSV.dial PSR @18:34:02.60 PS.reconfigure :fast @18:34:02.64 PS.seek 2.5ml @18:34:26.51 PSV.dial PTV @18:34:27.28 delay 5 seconds @18:34:32.30 PSV.dial PSR @18:34:33.06 delay 5 seconds @18:34:38.08 PSV.dial PTV @18:34:38.84 delay 5 seconds @18:34:43.86 PSV.dial :air @18:34:44.75 PS.seek 0ml @18:35:08.08 PTV.dial :PRVmixing,via: PRV @18:35:09.40 PSV.dial :mixloop @18:35:10.29 PRV.dial :ab1 @18:35:11.05 PS.reconfigure :slow @18:35:11.10 PS.seek 0.15ml @18:35:14.70 delay 2 seconds @18:35:16.72 CBV.dial PRV @18:35:16.75
PRV.dial CBV @18:35:17.33 PS.seek 0.35ml @18:35:21.95 delay 2 seconds @18:35:23.97 CBV.dial between PRV and PSV @18:35:24.29 PRV.dial :air @18:35:24.75 PS.seek 0.9ml @18:35:36.54 PRV.dial :waste2 @18:35:37.28 PS.seek 0.5ml @18:35:45.95 addPMLReagent08 @18:35:45.96 PSV.dial :air @18:35:46.42 PS.reconfigure :default @18:35:46.47 PS.seek 2ml @18:36:02.70 PSV.dial :PML @18:36:03.17 PTV.dial :mixing @18:36:03.92 PBV.dial :waste1 @18:36:04.34 PS.seek 1.85ml @18:36:06.76 PBV.dial between PSV and waste1 @18:36:07.13 PS.seek 0ml @18:36:26.92 delay 2 seconds @18:36:28.93 Beginning samplePassC @18:36:28.95 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @18:36:29.14 Elbow.reconfigure :tight @18:36:29.18 Elbow.seek FlushPuck::Garage @18:36:30.57 Elbow.reconfigure :loose @18:36:30.67 Forearm.seek :garage @18:36:31.65 Hand.close @18:36:32.41 Forearm.seek :retract @18:36:33.37 Elbow.reconfigure :tight @18:36:33.42 Elbow.seek CC @18:36:36.30 Elbow.reconfigure :loose @18:36:36.40 Forearm.reconfigure :tight @18:36:36.45 Forearm.seek CC @18:36:38.17 CC.seek :closed @18:36:42.69 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.48 @18:36:42.76 Hand.open @18:36:43.48 Forearm.reconfigure :loose @18:36:43.52 Forearm.seek :retract @18:36:44.49 PSV.dial PSR @18:36:45.56 Pulling 1.5ml of PSR.flush @18:36:45.57 PSR.select :flush @18:36:45.69 PS.seek 1.5ml @18:37:01.82 delay 5 seconds @18:37:06.84 PSV.dial :waste1 @18:37:07.33 PS.seek 0ml @18:37:23.66 PSV.dial PSR @18:37:24.14 PSR.select :air @18:37:24.25 PS.seek 2.5ml @18:37:50.79 PRV.dial CBV,avoiding: :ab2 @18:37:50.82
CBV.dial PRV @18:37:52.45 PSV.dial :mixloop @18:37:53.49 CTV.dial :air,avoiding: :intake @18:37:53.57 PTV.dial :PRVmixing @18:37:54.34 PS.seek 0ml @18:38:20.63 PSV.dial :air @18:38:21.22 PS.seek 2.5ml @18:38:47.66 PSV.dial :mixloop @18:38:48.12 PS.seek 0ml @18:39:14.44 PSV.dial :air @18:39:14.92 PS.seek 2.5ml @18:39:41.32 PSV.dial :mixloop @18:39:41.79 PS.seek 0ml @18:40:08.16 Garaging FlushPuck in Collection Clamp @18:40:08.24 Elbow.reconfigure :tight @18:40:08.29 Elbow.seek CC @18:40:08.86 Elbow.reconfigure :loose @18:40:08.97 Forearm.seek CC @18:40:09.97 Hand.close @18:40:10.54 CC.seek :open @18:40:15.18 Forearm.seek :retract @18:40:16.13 Elbow.reconfigure :tight @18:40:16.18 Elbow.seek :garage @18:40:19.11 Elbow.reconfigure :loose @18:40:19.21 Forearm.reconfigure :tight @18:40:19.26 Forearm.seek :garage @18:40:20.20 Hand.open @18:40:20.85 Forearm.reconfigure :loose @18:40:20.90 Forearm.seek :retract @18:40:21.85 delayUntil "18:44:28.92" @18:40:21.88 Whole Cell Archive sampling 1000ml @18:40:21.90 Loading wcr puck from tube 3 into Collection Clamp @18:40:22.38 Elbow.reconfigure :tight @18:40:22.43 Elbow.seek 3 @18:40:24.38 Forearm.reconfigure :tight @18:40:24.43 Forearm.seek 3 + 450 counts @18:40:25.46 SE.reconfigure :pick @18:40:25.52 SE.seek :beamBreak @18:40:27.54 SE.configure :default @18:40:27.71 Elbow.seek 3 @18:40:27.90 Forearm.seek 3 + 300 counts @18:40:28.42 Forearm.reconfigure :loose @18:40:28.47 Hand.close @18:40:29.05 SE.seek :down @18:40:29.27 Forearm.seek :retract @18:40:30.21 Elbow.seek CC @18:40:30.62 SE.stop @18:40:31.83 Elbow.reconfigure :loose @18:40:31.94 Forearm.reconfigure :tight @18:40:31.99 Forearm.seek CC @18:40:33.20 CC.seek :closed @18:40:37.74 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.30 @18:40:37.75 Hand.open @18:40:38.48 Forearm.reconfigure :loose @18:40:38.53 Forearm.seek :retract @18:40:39.46 collectSample 1000ml w/bubble point of 35psi @18:40:39.48 Priming 7ml @18:40:39.49 Intake.select :close @18:40:39.50 Exhaust.select :close @18:40:39.51 SS.hold @18:40:39.72 Calibrating pressure sensors @18:40:39.73 CTV.dial :air @18:40:39.82 SSV.dial :spit,avoiding: :exhaust @18:40:39.91 SS.reconfigure :fast @18:40:39.96 SS.seek 5ml @18:40:42.98 SSV.dial CBV @18:40:43.01 CBV.dial SSV @18:40:44.38 delay 3.8 seconds @18:40:48.31 Sensor limits: Intake max 110.0psia; Outlet max 102.3psia @18:40:48.76 SSV.dial :spit @18:40:50.08 SS.seek 0ml @18:40:52.62 SSV.dial CBV @18:40:53.94 CTV.dial :intake @18:40:54.71 SSV.dial CBV @18:40:54.74 CBV.dial SSV @18:40:54.82 Allowing 40s for 7.0ml of remaining 7.0ml @18:40:54.83 Intake.select :open @18:40:57.00 Intake @14.7psia, vacuum from 9.2 to 12.2psi @18:40:57.02 SS.reconfigure :inhaling 2.20ml/s @18:40:57.07 SS.seek 7ml @18:41:03.81 Stroke completed -- waiting for pressure to equalize ... @18:41:03.82 SS.reconfigure :exhale @18:41:03.87 SS.hold @18:41:16.58 Intake.select :close @18:41:16.74 SSV.dial :exhaust @18:41:17.95 Exhaust.select :open @18:41:18.07 SS.seek 0ml @18:41:22.28 Exhaust.select :close @18:41:22.39 Sampling 1000.0ml @18:41:22.40 Intake.select :close @18:41:22.41 Exhaust.select :close @18:41:22.42 SS.reconfigure :default @18:41:22.47 SS.hold @18:41:22.68 CTV.dial :intake @18:41:22.76 SSV.dial CBV @18:41:22.80 CBV.dial SSV @18:41:23.54 Allowing 130s for 25.0ml of remaining 1000.0ml @18:41:23.56 Intake.select :open @18:41:25.73 Intake @15.6psia, vacuum from 10.0 to 13.0psi @18:41:25.75 SS.reconfigure :inhaling 2.20ml/s @18:41:25.80 SS.seek 25ml @18:41:33.89 Vacuum too high -- waiting up to 40 seconds @18:41:33.91 SS.reconfigure :exhale @18:41:33.96 SS.hold @18:41:40.86 Intake @17.2psia, vacuum from 10.0 to 13.0psi @18:41:40.88 SS.reconfigure :inhaling 1.76ml/s @18:41:40.93 SS.seek 25ml @18:41:44.55 Vacuum too high -- waiting up to 40 seconds @18:41:44.57 SS.reconfigure :exhale @18:41:44.62 SS.hold @18:41:47.82 Intake @17.3psia, vacuum from 10.0 to 13.0psi @18:41:47.84 SS.reconfigure :inhaling 1.41ml/s @18:41:47.89 SS.seek 25ml @18:41:52.67 Vacuum too high -- waiting up to 40 seconds @18:41:52.69 SS.reconfigure :exhale @18:41:52.74 SS.hold @18:41:55.89 Intake @17.5psia, vacuum from 10.0 to 13.0psi @18:41:55.91 SS.reconfigure :inhaling 1.13ml/s @18:41:55.95 SS.seek 25ml @18:41:57.94 Stroke completed -- waiting for pressure to equalize ... @18:41:57.95 SS.reconfigure :exhale @18:41:58.00 SS.hold @18:42:12.84 Intake.select :close @18:42:13.00 SSV.dial :exhaust @18:42:13.78 Exhaust.select :open @18:42:13.89 SS.seek 0ml @18:42:22.68 Exhaust.select :close @18:42:22.80 SSV.dial CBV @18:42:22.83 CBV.dial SSV @18:42:23.61 Allowing 130s for 25.0ml of remaining 975.0ml @18:42:23.62 Intake.select :open @18:42:25.78 Intake @15.8psia, vacuum from 10.0 to 13.0psi @18:42:25.80 SS.reconfigure :inhaling 1.13ml/s @18:42:25.85 SS.seek 25ml @18:42:50.73 Stroke completed -- waiting for pressure to equalize ... @18:42:50.74 SS.reconfigure :exhale @18:42:50.79 SS.hold @18:43:09.37 Intake.select :close @18:43:09.53 SSV.dial :exhaust @18:43:10.32 Exhaust.select :open @18:43:10.44 SS.seek 0ml @18:43:19.28 Exhaust.select :close @18:43:19.39 SSV.dial CBV @18:43:19.42 CBV.dial SSV @18:43:20.21 Allowing 130s for 25.0ml of remaining 950.0ml @18:43:20.23 Intake.select :open @18:43:22.40 Intake @16.0psia, vacuum from 10.0 to 13.0psi @18:43:22.42 SS.reconfigure :inhaling 1.13ml/s @18:43:22.47 SS.seek 25ml @18:43:47.34 Stroke completed -- waiting for pressure to equalize ... @18:43:47.35 SS.reconfigure :exhale @18:43:47.40 SS.hold @18:44:08.07 Intake.select :close @18:44:08.23 SSV.dial :exhaust @18:44:09.00 Exhaust.select :open @18:44:09.12 SS.seek 0ml @18:44:18.13 Exhaust.select :close @18:44:18.25 SSV.dial CBV @18:44:18.28 CBV.dial SSV @18:44:19.06 Allowing 130s for 25.0ml of remaining 925.0ml @18:44:19.07 Intake.select :open @18:44:21.23 Intake @15.9psia, vacuum from 10.0 to 13.0psi @18:44:21.25 SS.reconfigure :inhaling 1.13ml/s @18:44:21.30 SS.seek 25ml @18:44:28.94 recoverPRVreagent08 @18:44:28.95 PTV.dial :mixing @18:44:30.09 PBV.dial :waste1 @18:44:30.47 delay 30 seconds @18:44:44.95 Vacuum too high -- waiting up to 40 seconds @18:44:44.97 SS.reconfigure :exhale @18:44:45.03 SS.hold @18:44:51.65 Intake @16.2psia, vacuum from 10.0 to 13.0psi @18:44:51.67 SS.reconfigure :inhaling 0.91ml/s @18:44:51.72 SS.seek 25ml @18:44:52.38 Stroke completed -- waiting for pressure to equalize ... @18:44:52.39 SS.reconfigure :exhale @18:44:52.49 SS.hold @18:45:00.49 PBV.dial between PSV and waste1 @18:45:00.86 PSV.dial :air @18:45:01.33 PS.reconfigure :fast @18:45:01.38 PS.seek 2.5ml @18:45:03.73 Intake.select :close @18:45:03.89 SSV.dial :exhaust @18:45:04.67 Exhaust.select :open @18:45:04.79 SS.seek 0ml @18:45:13.52 Exhaust.select :close @18:45:13.64 SSV.dial CBV @18:45:13.68 CBV.dial SSV @18:45:14.43 Allowing 130s for 25.0ml of remaining 900.0ml @18:45:14.45 Intake.select :open @18:45:16.61 Intake @16.0psia, vacuum from 10.0 to 13.0psi @18:45:16.63 SS.reconfigure :inhaling 0.91ml/s @18:45:16.68 SS.seek 25ml @18:45:25.47 PSV.dial :mixloop @18:45:25.94 delay 2 seconds @18:45:27.96 PSV.dial :air @18:45:28.42 delay 2 seconds @18:45:30.44 PSV.dial :mixloop @18:45:30.91 delay 2 seconds @18:45:32.92 PSV.dial :air @18:45:33.39 PS.seek 0ml @18:45:46.72 Stroke completed -- waiting for pressure to equalize ... @18:45:46.74 SS.reconfigure :exhale @18:45:46.79 SS.hold @18:45:57.09 washCycleDA #1 of 2 @18:45:57.10 PSR.select :air @18:45:57.12 PSV.dial PSR @18:45:58.45 Pulling 0.5ml of PSR.wash @18:45:58.46 PSR.select :wash @18:45:58.58 PS.reconfigure :default @18:45:58.63 PS.seek 0.5ml @18:45:59.65 Intake.select :close @18:45:59.81 SSV.dial :exhaust @18:46:00.59 Exhaust.select :open @18:46:00.70 SS.seek 0ml @18:46:04.59 delay 5 seconds @18:46:09.49 Exhaust.select :close @18:46:09.61 SSV.dial CBV @18:46:09.65 CBV.dial SSV @18:46:09.75 PSR.select :air @18:46:09.88 PS.seek 2ml @18:46:10.43 Allowing 130s for 25.0ml of remaining 875.0ml @18:46:10.44 Intake.select :open @18:46:12.62 Intake @16.0psia, vacuum from 10.0 to 13.0psi @18:46:12.64 SS.reconfigure :inhaling 0.91ml/s @18:46:12.69 SS.seek 25ml @18:46:25.97 PTV.dial :puck @18:46:26.72 PSV.dial PTV @18:46:27.47 PBV.dial :waste1 @18:46:27.90 PS.seek 1.5ml @18:46:33.97 delay 3 seconds @18:46:36.99 PBV.dial between PSV and waste1 @18:46:37.38 PS.seek 0ml @18:46:42.77 Stroke completed -- waiting for pressure to equalize ... @18:46:42.78 SS.reconfigure :exhale @18:46:42.83 SS.hold @18:46:53.53 delay 2:00 @18:46:59.15 Intake.select :close @18:46:59.31 SSV.dial :exhaust @18:47:00.08 Exhaust.select :open @18:47:00.20 SS.seek 0ml @18:47:08.94 Exhaust.select :close @18:47:09.06 SSV.dial CBV @18:47:09.08 CBV.dial SSV @18:47:10.35 Allowing 130s for 25.0ml of remaining 850.0ml @18:47:10.36 Intake.select :open @18:47:12.53 Intake @15.8psia, vacuum from 10.0 to 13.0psi @18:47:12.55 SS.reconfigure :inhaling 0.91ml/s @18:47:12.60 SS.seek 25ml @18:47:42.58 Stroke completed -- waiting for pressure to equalize ... @18:47:42.59 SS.reconfigure :exhale @18:47:42.64 SS.hold @18:47:57.05 Intake.select :close @18:47:57.21 SSV.dial :exhaust @18:47:57.99 Exhaust.select :open @18:47:58.10 SS.seek 0ml @18:48:07.11 Exhaust.select :close @18:48:07.68 SSV.dial CBV @18:48:07.72 CBV.dial SSV @18:48:08.50 Allowing 130s for 25.0ml of remaining 825.0ml @18:48:08.52 Intake.select :open @18:48:10.69 Intake @15.9psia, vacuum from 10.0 to 13.0psi @18:48:10.71 SS.reconfigure :inhaling 0.91ml/s @18:48:10.76 SS.seek 25ml @18:48:40.22 Stroke completed -- waiting for pressure to equalize ... @18:48:40.23 SS.reconfigure :exhale @18:48:40.28 SS.hold @18:48:53.55 recoverPSRreagent08 @18:48:53.56 PTV.dial :puck @18:48:53.64 PBV.dial :waste1 @18:48:54.01 delay 30 seconds @18:48:54.73 Intake.select :close @18:48:54.94 SSV.dial :exhaust @18:48:56.16 Exhaust.select :open @18:48:56.28 SS.seek 0ml @18:49:05.35 Exhaust.select :close @18:49:05.46 SSV.dial CBV @18:49:05.50 CBV.dial SSV @18:49:06.25 Allowing 130s for 25.0ml of remaining 800.0ml @18:49:06.27 Intake.select :open @18:49:08.44 Intake @15.8psia, vacuum from 10.0 to 13.0psi @18:49:08.46 SS.reconfigure :inhaling 0.91ml/s @18:49:08.51 SS.seek 25ml @18:49:24.03 PBV.dial between PSV and waste1 @18:49:24.40 PSR.select :air @18:49:24.42 PSV.dial PSR @18:49:25.19 PS.reconfigure :fast @18:49:25.24 PS.seek 2.5ml @18:49:37.89 Stroke completed -- waiting for pressure to equalize ... @18:49:37.91 SS.reconfigure :exhale @18:49:37.95 SS.hold @18:49:49.37 PSV.dial PTV @18:49:50.61 delay 5 seconds @18:49:54.28 Intake.select :close @18:49:54.45 SSV.dial :exhaust @18:49:55.22 Exhaust.select :open @18:49:55.34 SS.seek 0ml @18:49:55.63 PSV.dial PSR @18:49:56.39 delay 5 seconds @18:50:01.41 PSV.dial PTV @18:50:02.17 delay 5 seconds @18:50:04.19 Exhaust.select :close @18:50:04.30 SSV.dial CBV @18:50:04.34 CBV.dial SSV @18:50:05.08 Allowing 130s for 25.0ml of remaining 775.0ml @18:50:05.09 Intake.select :open @18:50:07.18 PSV.dial :air @18:50:07.28 Intake @15.7psia, vacuum from 10.0 to 13.0psi @18:50:07.30 SS.reconfigure :inhaling 0.91ml/s @18:50:07.35 SS.seek 25ml @18:50:07.95 PS.seek 0ml @18:50:31.46 washCycleDA #2 of 2 @18:50:31.47 PSR.select :air @18:50:31.49 PSV.dial PSR @18:50:32.84 Pulling 0.5ml of PSR.wash @18:50:32.85 PSR.select :wash @18:50:32.97 PS.reconfigure :default @18:50:33.01 PS.seek 0.5ml @18:50:36.83 Stroke completed -- waiting for pressure to equalize ... @18:50:36.85 SS.reconfigure :exhale @18:50:36.90 SS.hold @18:50:38.99 delay 5 seconds @18:50:44.01 PSR.select :air @18:50:44.13 PS.seek 2ml @18:50:55.18 Intake.select :close @18:50:55.33 SSV.dial :exhaust @18:50:56.09 Exhaust.select :open @18:50:56.21 SS.seek 0ml @18:51:00.30 PTV.dial :puck @18:51:00.38 PSV.dial PTV @18:51:01.13 PBV.dial :waste1 @18:51:01.57 PS.seek 1.5ml @18:51:04.99 Exhaust.select :close @18:51:05.10 SSV.dial CBV @18:51:05.13 CBV.dial SSV @18:51:05.92 Allowing 130s for 25.0ml of remaining 750.0ml @18:51:05.94 Intake.select :open @18:51:07.56 delay 3 seconds @18:51:08.10 Intake @15.8psia, vacuum from 10.0 to 13.0psi @18:51:08.12 SS.reconfigure :inhaling 0.91ml/s @18:51:08.17 SS.seek 25ml @18:51:10.57 PBV.dial between PSV and waste1 @18:51:10.94 PS.seek 0ml @18:51:27.27 delay 2:00 @18:51:33.43 Vacuum too high -- waiting up to 40 seconds @18:51:33.44 SS.reconfigure :exhale @18:51:33.50 SS.hold @18:51:42.53 Intake @15.6psia, vacuum from 10.0 to 13.0psi @18:51:42.55 SS.reconfigure :inhaling 0.73ml/s @18:51:42.60 SS.seek 25ml @18:51:48.53 Stroke completed -- waiting for pressure to equalize ... @18:51:48.54 SS.reconfigure :exhale @18:51:48.59 SS.hold @18:51:59.82 Intake.select :close @18:51:59.98 SSV.dial :exhaust @18:52:00.75 Exhaust.select :open @18:52:00.87 SS.seek 0ml @18:52:10.00 Exhaust.select :close @18:52:10.11 SSV.dial CBV @18:52:10.15 CBV.dial SSV @18:52:10.91 Allowing 130s for 25.0ml of remaining 725.0ml @18:52:10.92 Intake.select :open @18:52:13.08 Intake @15.7psia, vacuum from 10.0 to 13.0psi @18:52:13.10 SS.reconfigure :inhaling 0.73ml/s @18:52:13.15 SS.seek 25ml @18:52:49.12 Stroke completed -- waiting for pressure to equalize ... @18:52:49.14 SS.reconfigure :exhale @18:52:49.18 SS.hold @18:53:08.16 Intake.select :close @18:53:08.32 SSV.dial :exhaust @18:53:09.11 Exhaust.select :open @18:53:09.22 SS.seek 0ml @18:53:18.22 Exhaust.select :close @18:53:18.33 SSV.dial CBV @18:53:18.36 CBV.dial SSV @18:53:19.14 Allowing 130s for 25.0ml of remaining 700.0ml @18:53:19.15 Intake.select :open @18:53:21.32 Intake @15.8psia, vacuum from 10.0 to 13.0psi @18:53:21.34 SS.reconfigure :inhaling 0.73ml/s @18:53:21.39 SS.seek 25ml @18:53:27.29 recoverPSRreagent08 @18:53:27.30 PTV.dial :puck @18:53:27.38 PBV.dial :waste1 @18:53:27.76 delay 30 seconds @18:53:50.22 Vacuum too high -- waiting up to 40 seconds @18:53:50.24 SS.reconfigure :exhale @18:53:50.30 SS.hold @18:53:57.82 PBV.dial between PSV and waste1 @18:53:58.01 Intake @15.3psia, vacuum from 9.8 to 12.8psi @18:53:58.03 SS.reconfigure :inhaling 0.59ml/s @18:53:58.08 SS.seek 25ml @18:53:58.25 PSR.select :air @18:53:58.27 PSV.dial PSR @18:53:59.04 PS.reconfigure :fast @18:53:59.09 PS.seek 2.5ml @18:54:07.42 Stroke completed -- waiting for pressure to equalize ... @18:54:07.43 SS.reconfigure :exhale @18:54:07.48 SS.hold @18:54:20.17 Intake.select :close @18:54:20.33 SSV.dial :exhaust @18:54:21.11 Exhaust.select :open @18:54:21.22 SS.seek 0ml @18:54:23.33 PSV.dial PTV @18:54:24.10 delay 5 seconds @18:54:29.12 PSV.dial PSR @18:54:29.91 delay 5 seconds @18:54:30.20 Exhaust.select :close @18:54:30.31 SSV.dial CBV @18:54:30.35 CBV.dial SSV @18:54:31.09 Allowing 130s for 25.0ml of remaining 675.0ml @18:54:31.10 Intake.select :open @18:54:33.27 Intake @15.7psia, vacuum from 10.0 to 13.0psi @18:54:33.29 SS.reconfigure :inhaling 0.59ml/s @18:54:33.34 SS.seek 25ml @18:54:34.93 PSV.dial PTV @18:54:35.71 delay 5 seconds @18:54:40.73 PSV.dial :air @18:54:41.49 PS.seek 0ml @18:55:04.93 getPMLReagent08 @18:55:04.94 PRV.dial :air @18:55:05.40 delay 5 seconds @18:55:10.42 PTV.dial :PRVmixing @18:55:11.74 delay 5 seconds @18:55:16.76 PRV.dial :ab2 @18:55:17.22 PTV.dial :PRVmixing @18:55:17.30 PSV.dial :mixloop @18:55:17.54 Stroke completed -- waiting for pressure to equalize ... @18:55:17.55 SS.reconfigure :exhale @18:55:17.60 SS.hold @18:55:17.79 PS.reconfigure :default @18:55:17.84 PS.seek 0.3ml @18:55:21.74 delay 2 seconds @18:55:23.76 PRV.dial :air @18:55:24.33 PS.seek 0.55ml @18:55:27.74 addPMLReagent08 @18:55:27.75 PSV.dial :air @18:55:28.21 PS.seek 2ml @18:55:38.14 Intake.select :close @18:55:38.30 SSV.dial :exhaust @18:55:39.09 Exhaust.select :open @18:55:39.20 SS.seek 0ml @18:55:43.86 PSV.dial :PML @18:55:44.34 PTV.dial :mixing @18:55:45.08 PBV.dial :waste1 @18:55:45.51 PS.seek 1.85ml @18:55:47.80 PBV.dial between PSV and waste1 @18:55:47.98 Exhaust.select :close @18:55:48.10 SSV.dial CBV @18:55:48.13 CBV.dial SSV @18:55:48.21 PS.seek 0ml @18:55:48.95 Allowing 130s for 25.0ml of remaining 650.0ml @18:55:48.96 Intake.select :open @18:55:51.12 Intake @15.8psia, vacuum from 10.0 to 13.0psi @18:55:51.55 SS.reconfigure :inhaling 0.59ml/s @18:55:51.66 SS.seek 25ml @18:56:08.02 delay 2 seconds @18:56:10.04 delay 8:00 @18:56:27.00 Vacuum too high -- waiting up to 40 seconds @18:56:27.02 SS.reconfigure :exhale @18:56:27.08 SS.hold @18:56:38.38 Intake @16.2psia, vacuum from 10.0 to 13.0psi @18:56:38.40 SS.reconfigure :inhaling 0.47ml/s @18:56:38.45 SS.seek 25ml @18:56:50.73 Stroke completed -- waiting for pressure to equalize ... @18:56:50.74 SS.reconfigure :exhale @18:56:50.79 SS.hold @18:57:03.95 Intake.select :close @18:57:04.11 SSV.dial :exhaust @18:57:04.88 Exhaust.select :open @18:57:05.00 SS.seek 0ml @18:57:14.06 Exhaust.select :close @18:57:14.17 SSV.dial CBV @18:57:14.21 CBV.dial SSV @18:57:14.98 Allowing 130s for 25.0ml of remaining 625.0ml @18:57:14.99 Intake.select :open @18:57:17.16 Intake @15.7psia, vacuum from 10.0 to 13.0psi @18:57:17.18 SS.reconfigure :inhaling 0.47ml/s @18:57:17.23 SS.seek 25ml @18:58:12.29 Stroke completed -- waiting for pressure to equalize ... @18:58:12.30 SS.reconfigure :exhale @18:58:12.35 SS.hold @18:58:37.09 Intake.select :close @18:58:37.25 SSV.dial :exhaust @18:58:38.01 Exhaust.select :open @18:58:38.12 SS.seek 0ml @18:58:47.29 Exhaust.select :close @18:58:47.40 SSV.dial CBV @18:58:47.42 CBV.dial SSV @18:58:48.23 Allowing 130s for 25.0ml of remaining 600.0ml @18:58:48.24 Intake.select :open @18:58:50.41 Intake @15.7psia, vacuum from 10.0 to 13.0psi @18:58:50.43 SS.reconfigure :inhaling 0.47ml/s @18:58:50.48 SS.seek 25ml @18:59:45.36 Stroke completed -- waiting for pressure to equalize ... @18:59:45.77 SS.reconfigure :exhale @18:59:45.89 SS.hold @19:00:14.50 Intake.select :close @19:00:15.13 SSV.dial :exhaust @19:00:15.90 Exhaust.select :open @19:00:16.01 SS.seek 0ml @19:00:24.75 Exhaust.select :close @19:00:24.87 SSV.dial CBV @19:00:24.90 CBV.dial SSV @19:00:25.69 Allowing 130s for 25.0ml of remaining 575.0ml @19:00:25.71 Intake.select :open @19:00:27.88 Intake @15.7psia, vacuum from 10.0 to 13.0psi @19:00:27.90 SS.reconfigure :inhaling 0.47ml/s @19:00:27.95 SS.seek 25ml @19:01:13.87 Vacuum too high -- waiting up to 40 seconds @19:01:13.89 SS.reconfigure :exhale @19:01:13.94 SS.hold @19:01:30.39 Intake @16.1psia, vacuum from 10.0 to 13.0psi @19:01:30.41 SS.reconfigure :inhaling 0.38ml/s @19:01:30.46 SS.seek 25ml @19:01:42.80 Stroke completed -- waiting for pressure to equalize ... @19:01:42.81 SS.reconfigure :exhale @19:01:42.86 SS.hold @19:02:01.25 Intake.select :close @19:02:01.40 SSV.dial :exhaust @19:02:02.19 Exhaust.select :open @19:02:02.78 SS.seek 0ml @19:02:11.89 Exhaust.select :close @19:02:12.01 SSV.dial CBV @19:02:12.05 CBV.dial SSV @19:02:12.80 Allowing 130s for 25.0ml of remaining 550.0ml @19:02:12.81 Intake.select :open @19:02:14.98 Intake @15.8psia, vacuum from 10.0 to 13.0psi @19:02:15.00 SS.reconfigure :inhaling 0.38ml/s @19:02:15.05 SS.seek 25ml @19:03:23.50 Stroke completed -- waiting for pressure to equalize ... @19:03:23.51 SS.reconfigure :exhale @19:03:23.56 SS.hold @19:03:58.80 Intake.select :close @19:03:58.96 SSV.dial :exhaust @19:03:59.74 Exhaust.select :open @19:03:59.85 SS.seek 0ml @19:04:08.67 Exhaust.select :close @19:04:08.79 SSV.dial CBV @19:04:08.82 CBV.dial SSV @19:04:09.59 Allowing 130s for 25.0ml of remaining 525.0ml @19:04:09.61 Intake.select :open @19:04:10.05 recoverPRVreagent08 @19:04:10.06 PTV.dial :mixing @19:04:10.13 PBV.dial :waste1 @19:04:10.51 delay 30 seconds @19:04:11.78 Intake @15.8psia, vacuum from 10.0 to 13.0psi @19:04:11.80 SS.reconfigure :inhaling 0.38ml/s @19:04:11.85 SS.seek 25ml @19:04:40.53 PBV.dial between PSV and waste1 @19:04:40.91 PSV.dial :air @19:04:41.39 PS.reconfigure :fast @19:04:41.44 PS.seek 2.5ml @19:05:05.34 Vacuum too high -- waiting up to 40 seconds @19:05:05.35 SS.reconfigure :exhale @19:05:05.41 SS.hold @19:05:05.78 PSV.dial :mixloop @19:05:06.26 delay 2 seconds @19:05:08.27 PSV.dial :air @19:05:08.74 delay 2 seconds @19:05:10.76 PSV.dial :mixloop @19:05:11.23 delay 2 seconds @19:05:13.24 PSV.dial :air @19:05:13.84 PS.seek 0ml @19:05:24.19 Intake @16.6psia, vacuum from 10.0 to 13.0psi @19:05:24.21 SS.reconfigure :inhaling 0.31ml/s @19:05:24.26 SS.seek 25ml @19:05:37.42 washCycleDA #1 of 3 @19:05:37.43 PSR.select :air @19:05:37.44 PSV.dial PSR @19:05:38.79 Pulling 0.5ml of PSR.wash @19:05:38.80 PSR.select :wash @19:05:38.92 PS.reconfigure :default @19:05:38.97 PS.seek 0.5ml @19:05:44.07 Stroke completed -- waiting for pressure to equalize ... @19:05:44.09 SS.reconfigure :exhale @19:05:44.14 SS.hold @19:05:44.99 delay 5 seconds @19:05:50.03 PSR.select :air @19:05:50.15 PS.seek 2ml @19:06:06.28 PTV.dial :puck @19:06:07.05 PSV.dial PTV @19:06:07.85 PBV.dial :waste1 @19:06:08.29 PS.seek 1.5ml @19:06:09.25 Intake.select :close @19:06:09.41 SSV.dial :exhaust @19:06:10.19 Exhaust.select :open @19:06:10.31 SS.seek 0ml @19:06:14.36 delay 3 seconds @19:06:17.38 PBV.dial between PSV and waste1 @19:06:17.76 PS.seek 0ml @19:06:19.90 Exhaust.select :close @19:06:20.01 SSV.dial CBV @19:06:20.05 CBV.dial SSV @19:06:20.83 Allowing 130s for 25.0ml of remaining 500.0ml @19:06:20.84 Intake.select :open @19:06:23.01 Intake @15.6psia, vacuum from 10.0 to 13.0psi @19:06:23.03 SS.reconfigure :inhaling 0.31ml/s @19:06:23.08 SS.seek 25ml @19:06:33.91 delay 2:00 @19:07:50.57 Stroke completed -- waiting for pressure to equalize ... @19:07:50.59 SS.reconfigure :exhale @19:07:50.63 SS.hold @19:08:30.82 Intake.select :close @19:08:30.98 SSV.dial :exhaust @19:08:31.76 Exhaust.select :open @19:08:31.88 SS.seek 0ml @19:08:33.93 recoverPSRreagent08 @19:08:33.94 PTV.dial :puck @19:08:33.96 PBV.dial :waste1 @19:08:34.33 delay 30 seconds @19:08:40.70 Exhaust.select :close @19:08:40.81 SSV.dial CBV @19:08:40.84 CBV.dial SSV @19:08:41.64 Allowing 130s for 25.0ml of remaining 475.0ml @19:08:41.65 Intake.select :open @19:08:43.82 Intake @15.6psia, vacuum from 10.0 to 13.0psi @19:08:43.84 SS.reconfigure :inhaling 0.31ml/s @19:08:43.89 SS.seek 25ml @19:09:04.36 PBV.dial between PSV and waste1 @19:09:04.72 PSR.select :air @19:09:04.74 PSV.dial PSR @19:09:05.51 PS.reconfigure :fast @19:09:05.56 PS.seek 2.5ml @19:09:29.53 PSV.dial PTV @19:09:30.43 delay 5 seconds @19:09:35.45 PSV.dial PSR @19:09:36.19 delay 5 seconds @19:09:41.21 PSV.dial PTV @19:09:41.97 delay 5 seconds @19:09:46.99 PSV.dial :air @19:09:47.77 PS.seek 0ml @19:10:11.75 washCycleDA #2 of 3 @19:10:11.76 PSR.select :air @19:10:11.78 PSV.dial PSR @19:10:13.07 Stroke completed -- waiting for pressure to equalize ... @19:10:13.08 SS.reconfigure :exhale @19:10:13.15 Pulling 0.5ml of PSR.wash @19:10:13.17 SS.hold @19:10:13.19 PSR.select :wash @19:10:13.46 PS.reconfigure :default @19:10:13.52 PS.seek 0.5ml @19:10:19.47 delay 5 seconds @19:10:24.48 PSR.select :air @19:10:24.60 PS.seek 2ml @19:10:40.87 PTV.dial :puck @19:10:40.89 PSV.dial PTV @19:10:41.64 PBV.dial :waste1 @19:10:42.08 PS.seek 1.5ml @19:10:48.12 delay 3 seconds @19:10:51.14 PBV.dial between PSV and waste1 @19:10:51.52 PS.seek 0ml @19:10:51.66 SS.reconfigure :default @19:10:51.72 SS.hold @19:10:51.78 Intake.select :close @19:10:51.94 ShallowSampler::Clogged in WCR -- Filtering too Slowly after 550.0ml @19:10:51.96 SSV.dial :exhaust @19:10:52.74 Exhaust.select :open @19:10:52.86 SS.reconfigure :exhale @19:10:52.91 SS.seek 0ml @19:11:02.18 Exhaust.select :close @19:11:02.29 Sampled 550.0ml @19:11:02.31 Drying puck before fixing for 5:00 @19:11:02.32 CBV.dial :waste2b @19:11:03.08 delay 1:00 @19:11:07.74 delay 2:00 @19:12:03.11 CSV.dial :air @19:12:03.61 CS.reconfigure :fast @19:12:03.66 CS.seek 10ml @19:12:27.97 Pumping wcr puck from tube 3 up to 35psi... @19:12:27.99 CBV.dial SSV @19:12:29.26 CBV.dial between CSV and waste2b @19:12:30.22 CSV.dial between air and CTV @19:12:30.54 CS.seek 7.151ml @19:12:37.88 CTV.dial CSV,avoiding: :air @19:12:37.91 CSV.dial CTV @19:12:39.20 CS.reconfigure :pressurize @19:12:39.26 CS.seek 0ml @19:12:55.68 CBV.dial :waste2b @19:12:56.01 CS.reconfigure :default @19:12:56.06 CS.seek 0ml @19:12:59.32 delay 1:00 @19:13:07.76 recoverPSRreagent08 @19:13:07.77 PTV.dial :puck @19:13:07.79 PBV.dial :waste1 @19:13:08.16 delay 30 seconds @19:13:38.18 PBV.dial between PSV and waste1 @19:13:38.56 PSR.select :air @19:13:38.58 PSV.dial PSR @19:13:39.35 PS.reconfigure :fast @19:13:39.40 PS.seek 2.5ml @19:13:59.34 CTV.dial :air,avoiding: :intake @19:14:00.68 Fix #1 of 2 (with RNAlater) for 2:30 @19:14:00.69 CSV.dial CSR @19:14:01.13 Pulling 2ml of CSR.RNAlater @19:14:01.14 CSR.select :RNAlater @19:14:01.26 CS.seek 2ml @19:14:03.31 PSV.dial PTV @19:14:04.08 delay 5 seconds @19:14:07.28 delay 5 seconds @19:14:09.09 PSV.dial PSR @19:14:09.84 delay 5 seconds @19:14:12.30 CSR.select :air @19:14:12.43 CS.reconfigure :fast @19:14:12.48 CS.seek 10ml @19:14:14.86 PSV.dial PTV @19:14:15.62 delay 5 seconds @19:14:20.64 PSV.dial :air @19:14:21.52 PS.seek 0ml @19:14:32.25 Pumping wcr puck from tube 3 up to 35psi... @19:14:32.27 CBV.dial SSV @19:14:33.52 CBV.dial between CSV and waste2b @19:14:34.49 CSV.dial between air and CTV @19:14:35.10 CS.seek 7.085ml @19:14:42.51 CTV.dial CSV,avoiding: :air @19:14:42.55 CSV.dial CTV @19:14:43.93 CS.reconfigure :pressurize @19:14:43.98 CS.seek 0ml @19:14:44.97 washCycleDA #3 of 3 @19:14:44.98 PSR.select :air @19:14:44.99 PSV.dial PSR @19:14:46.34 Pulling 0.5ml of PSR.wash @19:14:46.35 PSR.select :wash @19:14:46.47 PS.reconfigure :default @19:14:46.52 PS.seek 0.5ml @19:14:52.43 delay 5 seconds @19:14:57.45 PSR.select :air @19:14:58.04 delay 2:30 @19:14:58.10 PS.seek 2ml @19:15:14.24 PTV.dial :puck @19:15:14.26 PSV.dial PTV @19:15:15.15 PBV.dial :waste1 @19:15:15.59 PS.seek 1.5ml @19:15:21.50 delay 3 seconds @19:15:24.52 PBV.dial between PSV and waste1 @19:15:24.89 PS.seek 0ml @19:15:41.03 delay 2:00 @19:17:28.07 CBV.dial :waste2b @19:17:28.41 CS.reconfigure :default @19:17:28.46 CS.seek 0ml @19:17:34.37 delay 1:00 @19:17:41.05 recoverPSRreagent08 @19:17:41.06 PTV.dial :puck @19:17:41.08 PBV.dial :waste1 @19:17:41.45 delay 30 seconds @19:18:11.47 PBV.dial between PSV and waste1 @19:18:11.85 PSR.select :air @19:18:11.87 PSV.dial PSR @19:18:12.62 PS.reconfigure :fast @19:18:12.68 PS.seek 2.5ml @19:18:34.40 CTV.dial :air,avoiding: :intake @19:18:35.74 Fix #2 of 2 (with RNAlater) for 2:30 @19:18:35.76 CSV.dial CSR @19:18:36.19 Pulling 2ml of CSR.RNAlater @19:18:36.20 CSR.select :RNAlater @19:18:36.32 CS.seek 2ml @19:18:36.65 PSV.dial PTV @19:18:37.41 delay 5 seconds @19:18:42.25 delay 5 seconds @19:18:42.43 PSV.dial PSR @19:18:43.18 delay 5 seconds @19:18:47.27 CSR.select :air @19:18:47.39 CS.reconfigure :fast @19:18:47.44 CS.seek 10ml @19:18:48.20 PSV.dial PTV @19:18:48.96 delay 5 seconds @19:18:53.98 PSV.dial :air @19:18:54.75 PS.seek 0ml @19:19:07.22 Pumping wcr puck from tube 3 up to 35psi... @19:19:07.24 CBV.dial SSV @19:19:08.51 CBV.dial between CSV and waste2b @19:19:09.48 CSV.dial between air and CTV @19:19:10.08 CS.seek 7.085ml @19:19:17.56 CTV.dial CSV,avoiding: :air @19:19:17.59 CSV.dial CTV @19:19:18.42 Gate.power :camera,:ON @19:19:18.92 Mixing DA Substrates @19:19:18.93 PSR.select :air @19:19:18.95 PSV.dial PSR @19:19:19.03 CS.reconfigure :pressurize @19:19:19.08 CS.seek 0ml @19:19:20.35 Pulling 0.25ml of PSR.sub1 @19:19:20.38 PSR.select :sub1 @19:19:20.59 PS.reconfigure :default @19:19:20.71 PS.seek 0.25ml @19:19:24.08 delay 5 seconds @19:19:29.11 Pulling 0.25ml of PSR.sub2 @19:19:29.12 PSR.select :sub2 @19:19:29.24 PS.seek 0.5ml @19:19:32.68 delay 5 seconds @19:19:37.02 delay 2:30 @19:19:37.69 PSR.select :air @19:19:37.81 PS.seek 2ml @19:19:54.02 PTV.dial :puck @19:19:54.03 PSV.dial PTV @19:19:54.91 PBV.dial :waste1 @19:19:55.36 PS.seek 1.5ml @19:20:01.40 delay 3 seconds @19:20:04.42 PBV.dial between PSV and waste1 @19:20:04.80 PS.seek 0ml @19:20:20.86 delay 10 seconds @19:20:30.89 recoverPSRreagent08 @19:20:30.90 PTV.dial :puck @19:20:30.91 PBV.dial :waste1 @19:20:31.29 delay 30 seconds @19:21:01.31 PBV.dial between PSV and waste1 @19:21:01.69 PSR.select :air @19:21:01.71 PSV.dial PSR @19:21:02.48 PS.reconfigure :fast @19:21:02.53 PS.seek 2.5ml @19:21:26.86 PSV.dial PTV @19:21:27.65 delay 5 seconds @19:21:32.67 PSV.dial PSR @19:21:33.44 delay 5 seconds @19:21:38.46 PSV.dial PTV @19:21:39.35 delay 5 seconds @19:21:44.37 PSV.dial :air @19:21:45.28 PS.seek 0ml @19:22:07.04 CBV.dial :waste2b @19:22:07.37 CS.reconfigure :default @19:22:07.43 CS.seek 0ml @19:22:09.00 delay 1:00 @19:22:09.18 Additional air clear @19:22:09.19 PSR.select :air @19:22:09.21 PSV.dial PSR @19:22:10.58 Pulling 2ml of PSR.air @19:22:10.59 PSR.select :air @19:22:10.61 PS.reconfigure :default @19:22:10.66 PS.seek 2ml @19:22:32.08 delay 5 seconds @19:22:37.10 PTV.dial :puck @19:22:37.12 PSV.dial PTV @19:22:37.89 PBV.dial :waste1 @19:22:38.33 PS.seek 1.5ml @19:22:44.30 delay 3 seconds @19:22:47.32 PBV.dial between PSV and waste1 @19:22:47.69 delay 1 second @19:22:48.71 PSV.dial :waste1 @19:22:49.78 PS.seek 0ml @19:23:05.98 Processing Puck Heater at 28C before imaging @19:23:05.99 PH.coast @19:23:06.59 Imaging da puck in Processing Clamp while storing it to tube 2 @19:23:06.78 SC.reconfigure :tight @19:23:06.83 SC.seek 2 @19:23:09.02 CTV.dial :air,avoiding: :intake @19:23:10.42 SC.configure :limp @19:23:10.64 Elbow.reconfigure :tight @19:23:10.69 Elbow.seek 2 @19:23:12.45 Forearm.reconfigure :tight @19:23:12.50 Forearm.seek 2 + 450 counts @19:23:13.50 SE.reconfigure :pick @19:23:13.56 SE.seek :beamBreak @19:23:15.84 SE.configure :default @19:23:16.01 Elbow.reconfigure :limp @19:23:16.06 Forearm.reconfigure :loose @19:23:16.11 Forearm.seek 2 @19:23:16.33 Elbow.hold @19:23:16.39 Elbow.reconfigure :tight @19:23:16.50 SE.seek 12.1 Pucks below Top Plate @19:23:16.97 Forearm.force -20 @19:23:17.02 Forearm.seek 2 + 318 counts @19:23:17.33 Forearm.reconfigure :tight @19:23:17.38 Forearm.seek 2 + 450 counts @19:23:17.60 SE.seek 13.1 Pucks below Top Plate @19:23:18.15 Forearm.reconfigure :loose @19:23:18.20 Forearm.seek :retract @19:23:19.18 Elbow.seek PC @19:23:21.67 Elbow.reconfigure :loose @19:23:21.77 Forearm.seek PC @19:23:22.75 Hand.close @19:23:23.38 PC.seek :open @19:23:28.67 Forearm.seek :retract @19:23:29.63 Elbow.reconfigure :tight @19:23:29.68 Elbow.seek 2 @19:23:32.31 Forearm.reconfigure :tight @19:23:32.36 Forearm.seek 2 + 450 counts @19:23:33.45 Hand.open @19:23:34.19 SE.seek 12.75 Pucks below Top Plate @19:23:34.45 SE.reconfigure :seal @19:23:34.50 SE.seek 11.79 Pucks below Top Plate @19:23:35.03 Lights, camera, ACTION! @19:23:35.06 PuckCamera: snap -bin=2,2 -auto /var/log/esp/da23oct2419h775ml.tif @19:23:54.56 Waiting >20 seconds for da to relinquish FlushPuck @19:23:56.91 SX HX9/16 (USB:12): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 18.551 seconds /var/log/esp/da23oct2419h775ml.tif: TIFF Upload Complete @19:23:56.94 PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da23oct2419h775ml15s.tif @19:24:12.59 SX HX9/16 (USB:12): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15 seconds /var/log/esp/midres/da23oct2419h775ml15s.tif: TIFF Upload Complete @19:24:12.62 PuckCamera: snap -bin=2,2 20.0 /var/log/esp/midres/da23oct2419h775ml20s.tif @19:24:33.35 SX HX9/16 (USB:12): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 20 seconds /var/log/esp/midres/da23oct2419h775ml20s.tif: TIFF Upload Complete @19:24:33.37 Gate.power :camera,:OFF @19:24:33.39 DAfans.email "18.551 sec auto-exposure",Subject: "da23oct2419h775ml images!" @19:24:33.83 Elbow.seek 2 @19:24:34.07 Forearm.seek 2 + 450 counts @19:24:34.16 SE.reconfigure :gently @19:24:34.22 SE.seek 12.51 Pucks below Top Plate @19:24:34.87 Elbow.reconfigure :limp @19:24:34.94 Forearm.reconfigure :loose @19:24:34.99 Forearm.seek 2 @19:24:35.24 Elbow.hold @19:24:35.31 Elbow.reconfigure :tight @19:24:35.38 SE.reconfigure :default @19:24:35.43 SE.seek 12.69 Pucks below Top Plate @19:24:35.92 Forearm.force -20 @19:24:36.02 Forearm.seek 2 + 300 counts @19:24:36.42 Forearm.reconfigure :tight @19:24:36.47 Forearm.seek 2 + 450 counts @19:24:36.74 SE.seek :down @19:24:37.97 Forearm.reconfigure :loose @19:24:38.04 Forearm.seek :retract @19:24:38.84 SE.stop @19:24:39.13 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @19:24:39.68 Elbow.seek FlushPuck::Garage @19:24:41.73 Elbow.reconfigure :loose @19:24:41.95 Forearm.seek :garage @19:24:43.05 Hand.close @19:24:44.08 Forearm.seek :retract @19:24:45.14 Elbow.reconfigure :tight @19:24:45.23 Elbow.seek PC @19:24:46.81 Elbow.reconfigure :loose @19:24:47.03 Forearm.reconfigure :tight @19:24:47.13 Forearm.seek PC @19:24:48.80 PC.seek :closed @19:24:53.64 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.62 @19:24:53.80 Hand.open @19:24:54.63 Forearm.reconfigure :loose @19:24:54.71 Forearm.seek :retract @19:24:55.77 PSR.select :air @19:24:55.83 Storing wcr puck in Collection Clamp to tube 2 @19:24:55.87 PSV.dial PSR @19:24:56.37 SC.reconfigure :tight @19:24:56.55 SC.seek 2 @19:24:56.67 Pulling 0.5ml of PSR.flush @19:24:56.71 PSR.select :flush @19:24:57.85 SC.configure :limp @19:24:58.12 PS.seek 0.5ml @19:24:58.47 Elbow.reconfigure :tight @19:24:58.57 Elbow.seek 2 @19:25:01.31 Forearm.reconfigure :tight @19:25:01.43 Forearm.seek 2 + 450 counts @19:25:02.57 SE.reconfigure :pick @19:25:02.71 SE.seek :beamBreak @19:25:04.13 delay 5 seconds @19:25:05.06 SE.configure :default @19:25:05.40 Elbow.reconfigure :limp @19:25:05.50 Forearm.reconfigure :loose @19:25:05.60 Forearm.seek 2 @19:25:05.88 Elbow.hold @19:25:06.09 Elbow.reconfigure :tight @19:25:06.29 SE.seek 13.1 Pucks below Top Plate @19:25:06.97 Forearm.force -20 @19:25:07.10 Forearm.seek 2 + 303 counts @19:25:08.40 Forearm.reconfigure :tight @19:25:08.52 Forearm.seek 2 + 450 counts @19:25:08.80 SE.seek 14.1 Pucks below Top Plate @19:25:09.17 PSR.select :air @19:25:09.57 PS.seek 2.5ml @19:25:09.71 Forearm.reconfigure :loose @19:25:09.81 Forearm.seek :retract @19:25:10.77 Elbow.seek CC @19:25:11.33 upsync @19:25:12.37 Elbow.reconfigure :loose @19:25:12.86 Forearm.seek CC @19:25:13.92 Hand.close @19:25:14.50 CC.seek :open @19:25:19.27 Forearm.seek :retract @19:25:20.23 Elbow.reconfigure :tight @19:25:20.28 Elbow.seek 2 @19:25:22.05 Elbow.reconfigure :limp @19:25:22.11 Forearm.seek 2 @19:25:23.09 Elbow.hold @19:25:23.16 Elbow.reconfigure :tight @19:25:23.21 Hand.open @19:25:24.00 Forearm.force -20 @19:25:24.05 Forearm.seek 2 + 305 counts @19:25:24.36 Forearm.reconfigure :tight @19:25:24.41 Forearm.seek 2 + 450 counts @19:25:24.70 SE.seek :down @19:25:25.84 Forearm.reconfigure :loose @19:25:25.90 Forearm.seek :retract @19:25:26.46 SE.stop @19:25:26.88
Starting finalPurge @19:25:30.80 delay 1 second @19:25:31.82 PRV.dial :waste2,avoiding: :ab2 @19:25:33.67 PTV.dial :PRVmixing @19:25:34.98 PSV.dial :mixloop @19:25:36.03 PS.seek :empty @19:25:46.51
Waiting >20 seconds for psFlush to relinquish FlushPuck @19:26:02.42 PSR.select :air @19:26:02.44 PSV.dial PSR @19:26:03.48 Pulling 0.5ml of PSR.flush @19:26:03.50 PSR.select :flush @19:26:03.62 PS.seek 0.5ml @19:26:09.59 delay 5 seconds @19:26:14.61 PSR.select :air @19:26:14.73 PS.seek 2.5ml @19:26:36.17 delay 1 second @19:26:37.19 PBV.dial :waste1 @19:26:37.55 PTV.dial :mixing @19:26:38.30 PSV.dial :mixloop @19:26:39.35 PS.seek :empty @19:27:05.76 PSV.dial PSR @19:27:06.81 PSR.select :flush @19:27:06.93 PS.seek 1ml @19:27:18.04 PSR.select :air @19:27:18.17 PS.seek 2.5ml @19:27:34.26 PSV.dial PTV @19:27:35.14 PTV.dial :puck @19:27:35.87 PBV.dial :waste1 @19:27:35.89 PS.seek 0ml @19:28:02.29 PSV.dial PSR @19:28:03.04 PSR.select :flush @19:28:03.18 PS.seek 0.5ml @19:28:09.14 PSR.select :air @19:28:09.25 PS.seek 2.5ml @19:28:30.48 PSV.dial PTV @19:28:31.23 PBV.dial PSV @19:28:31.78 PS.seek 0ml @19:28:58.17 PSV.dial PBV @19:28:58.64 PS.seek 2.5ml @19:29:24.95 PSV.dial :waste1 @19:29:25.69 PS.seek 0ml @19:29:52.04 PSV.dial PSR @19:29:52.51 PSR.select :air @19:29:52.54 PS.seek 2.5ml @19:30:18.82 PSV.dial PTV @19:30:19.56 PBV.dial PSV @19:30:19.64 PS.seek 0ml @19:30:45.98 PSV.dial PBV @19:30:46.50 PS.seek 2.5ml @19:31:12.94 PSV.dial :waste1 @19:31:13.69 PS.seek 0ml @19:31:39.98 PSV.dial PSR @19:31:40.44 PSR.select :flush @19:31:40.57 PS.seek 1ml @19:31:51.60 PSR.select :air @19:31:51.72 PS.seek 2.5ml @19:32:07.88 PSV.dial PTV @19:32:09.00 PTV.dial :puck @19:32:09.08 PBV.dial :waste1 @19:32:09.62 PS.seek 0ml @19:32:36.01 PSV.dial PSR @19:32:36.76 PSR.select :flush @19:32:36.88 PS.seek 0.5ml @19:32:42.79 PSR.select :air @19:32:42.91 PS.seek 2.5ml @19:33:04.16 PSV.dial PTV @19:33:04.91 PBV.dial PSV @19:33:05.48 PS.seek 0ml @19:33:31.82 PSV.dial PBV @19:33:32.29 PS.seek 2.5ml @19:33:58.72 PSV.dial :waste1 @19:33:59.46 PS.seek 0ml @19:34:25.88 PSV.dial PSR @19:34:26.35 PSR.select :air @19:34:26.37 PS.seek 2.5ml @19:34:52.69 PSV.dial PTV @19:34:53.43 PBV.dial PSV @19:34:53.51 PS.seek 0ml @19:35:19.94 PSV.dial PBV @19:35:20.42 PS.seek 2.5ml @19:35:46.85 PSV.dial :waste1 @19:35:47.59 PS.seek 0ml @19:36:14.01
Loading FlushPuck into Collection Clamp from Processing Clamp @19:36:14.19 Elbow.seek PC @19:36:16.67 Elbow.reconfigure :loose @19:36:16.77 Forearm.seek PC @19:36:17.80 Hand.close @19:36:18.37 PC.seek :open @19:36:23.09 Forearm.seek :retract @19:36:24.10 Elbow.reconfigure :tight @19:36:24.15 Elbow.seek CC @19:36:27.72 Elbow.reconfigure :loose @19:36:27.82 Forearm.reconfigure :tight @19:36:27.86 Forearm.seek CC @19:36:29.52 CC.seek :closed @19:36:34.05 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.37 @19:36:34.11 Hand.open @19:36:34.80 Forearm.reconfigure :loose @19:36:34.85 Forearm.seek :retract @19:36:35.82 Flushing the Collection Valve::Manifold @19:36:35.95 CSV.dial CSR @19:36:36.40 Pulling 5ml of CSR.flush @19:36:36.41 CSR.select :flush @19:36:36.53 CS.seek 5ml @19:36:50.39 delay 5 seconds @19:36:55.40 CSR.select :air @19:36:55.57 CS.reconfigure :fast @19:36:55.62 CS.seek 7.5ml @19:37:02.38 CSV.dial :waste @19:37:03.67 CS.reconfigure :default @19:37:03.72 CS.seek 0ml @19:37:23.73 Flushing lower manifold's waste1 port @19:37:23.85 CSV.dial :flush @19:37:24.30 CS.reconfigure :fast @19:37:24.34 CS.seek 5ml @19:37:37.29 delay 5 seconds @19:37:42.31 CSV.dial :air @19:37:42.93 CS.seek 7ml @19:37:48.57 CSV.dial CTV @19:37:48.60
CTV.dial CSV @19:37:49.98 CBV.dial :waste1 @19:37:50.75 CS.seek 0ml @19:38:07.35 Flushing lower manifold's waste2 port @19:38:07.47 CSV.dial :flush @19:38:08.20 CS.seek 5ml @19:38:21.01 delay 5 seconds @19:38:26.03 CSV.dial :air @19:38:26.54 CS.seek 7.001ml @19:38:32.05 CSV.dial CTV @19:38:32.08
CTV.dial CSV @19:38:32.66 CBV.dial :waste2 @19:38:33.70 CS.seek 0ml @19:38:50.54 Flushing lower manifold's waste2b port @19:38:50.66 CSV.dial :flush @19:38:51.41 CS.seek 5ml @19:39:04.04 delay 5 seconds @19:39:09.06 CSV.dial :air @19:39:09.57 CS.seek 7.001ml @19:39:15.35 CSV.dial CTV @19:39:15.38
CTV.dial CSV @19:39:15.84 CBV.dial :waste2b @19:39:16.89 CS.seek 0ml @19:39:33.51 Disinfecting external Intake with kill @19:39:33.63 CSV.dial CSR @19:39:34.20 Pulling 8ml of CSR.kill CSR.select :kill @19:39:34.32 CS.seek 8ml @19:39:54.29 delay 5 seconds @19:39:59.31 CSR.select :air @19:39:59.43 CS.seek 10ml @19:40:04.99 CSV.dial CBV @19:40:05.02
CBV.dial CSV @19:40:06.09 CTV.dial :intake @19:40:06.87 Intake.select :open @19:40:06.98 CS.seek 2ml @19:40:25.91 Intake.select :close @19:40:26.01 CTV.dial :air @19:40:26.78 CS.reconfigure :default @19:40:26.83 CS.seek 0ml @19:40:32.75 CSV.dial CSR @19:40:33.77 Pulling 5ml of CSR.flush @19:40:33.78 CSR.select :flush @19:40:33.90 CS.seek 5ml @19:40:47.48 delay 5 seconds @19:40:52.49 CSR.select :air @19:40:52.61 CS.seek 10ml @19:41:06.19 CSV.dial CBV @19:41:07.20 CS.seek 0ml @19:41:33.63 Garaging FlushPuck in Collection Clamp @19:41:33.70 Elbow.reconfigure :tight @19:41:33.75 Elbow.seek CC @19:41:33.99 Elbow.reconfigure :loose @19:41:34.09 Forearm.seek CC @19:41:35.12 Hand.close @19:41:35.74 CC.seek :open @19:41:40.82 Forearm.seek :retract @19:41:41.78 Elbow.reconfigure :tight @19:41:41.83 Elbow.seek :garage @19:41:44.81 Elbow.reconfigure :loose @19:41:44.91 Forearm.reconfigure :tight @19:41:44.96 Forearm.seek :garage @19:41:45.89 Hand.open @19:41:46.55 Forearm.reconfigure :loose @19:41:46.59 Forearm.seek :retract @19:41:47.57 Completed [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] begun 17:16:28 @19:41:47.62 Asleep after completing DA_WCR_102423b assays @19:41:47.64 GoodNews.email "{1 => 33,\n 2 => 15,\n 3 => 18,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@19:35:39, 26% flow, 18.6C, 21% humidity, 14.6psia, 13.754V, 0.296A, 215.464Ah, 4.08W\nSampled 550.0ml",Subject: "Asleep after completing DA_WCR_102423b assays" @19:41:51.87 upsync + ESPwhich DA_WCR_102723.rb + fn=DA_WCR_102723.rb + IFS=: + set /home/esp/esp2/mission . + fullPath=/home/esp/esp2/mission/DA_WCR_102723.rb + [ -f /home/esp/esp2/mission/DA_WCR_102723.rb ] + realpath /home/esp/esp2/mission/DA_WCR_102723.rb + return 0 + scriptPath=/home/esp/esp2local/mission/DA_WCR_102723.rb + exec ruby /home/esp/esp2/lib/esp.rb /home/esp/esp2local/mission/DA_WCR_102723.rb @17:26:32.47UTC27-Oct-23
ESP2 v3.42 I2C Gateway v3.20 for core bus @17:26:35.08 Configuration for short puck jake 1/11/21 by brent@mbari.org /home/esp/esp2/type/shallow/jake/configure.rb @17:26:39.03 Polling Can every 10:00 @17:26:39.12 Cleanly exited at 17:23:09UTC27-Oct-23 @17:26:39.17 Loading /home/esp/esp2local/mission/DA_WCR_102723.rb mission @17:26:39.22 GoodNews.email "Starting DA_WCR_102723 w/pucks in tube 3\n{1 => 33,\n 2 => 15,\n 3 => 18,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@17:26:39, 25% flow, 20.4C, 21% humidity, 14.7psia, 13.739V, 0.284A, 231.192Ah, 3.91W",Subject: "DA_WCR_102723 begun" @17:26:39.25
Preparing to sleep until + 00:00:05 @17:26:39.30 Asleep til + 00:00:05 @17:26:39.31 Continuing at 17:26 @17:26:39.60 Gate.power :core,:OFF @17:26:39.61 Gate.power :raw,:OFF @17:26:39.62 Shutdown @17:26:41.76 GoodNews.email "Then, in tube 3: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_102723.rb:14\n{1 => 33,\n 2 => 15,\n 3 => 18,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@17:26:39, 25% flow, 20.4C, 21% humidity, 14.7psia, 13.739V, 0.296A, 231.192Ah, 4.07W",Subject: "Asleep until 17:26" @17:26:43.15 upsync @17:26:44.21
Powering core bus @17:26:45.67 upsync @17:26:49.77
Gate.power :raw,:ON @17:26:50.72 CS.configure :default @17:26:50.77 PS.configure :default @17:26:50.82 SS.configure :default @17:26:50.87 CC.configure :default @17:26:50.92 PC.configure :default @17:26:50.99 Forearm.configure :loose @17:26:51.04 Elbow.configure :loose @17:26:51.11 SC.configure :loose @17:26:51.16 SE.configure :default @17:26:51.20 CH.configure :default @17:26:51.24 PH.configure :default @17:26:51.28 All dwarf controllers running firmware version 3.85 @17:26:51.39 Valve.reset @17:26:52.00 Intake.select :close @17:26:52.05 Exhaust.select :close @17:26:52.09 CTV.dial :air @17:26:52.20 PSV.dial :air @17:26:52.32 CSV.dial :waste1 @17:26:52.35
Forearm.home.seek :retract @17:26:52.81 SSV.dial :spit,avoiding: :exhaust @17:26:53.12 Hand.close @17:26:53.16
Elbow.reconfigure :loose @17:26:53.25 Elbow.jog 1500 @17:26:53.32 PS.home.seek 0ml @17:26:53.69 CS.reconfigure :fast @17:26:53.73 SS.home.seek 0ml @17:26:53.83 CS.home.seek 0ml @17:26:53.91
Elbow.reconfigure :loose @17:26:53.96 Elbow.home.seek :clear @17:26:57.32 SE.home.seek :down @17:26:57.93 SC.reconfigure :tight @17:26:57.98 SC.home.seek :initial @17:27:01.55 PS.stop @17:27:10.58
SC.configure :limp @17:27:10.63 Forearm.seek :retract @17:27:10.70 Elbow.seek :hammer @17:27:11.70 Forearm.reconfigure :tight @17:27:11.75 Forearm.seek retracted @17:27:12.04 PC.home.seek :open @17:27:12.06
CC.home.seek :open @17:27:15.55 Phase [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] @17:27:15.56 Notice.email "At 17:27:15 began in tube 3: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_102723.rb:14\n{1 => 33,\n 2 => 15,\n 3 => 18,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@17:27:15, 25% flow, 20.4C, 21% humidity, 14.7psia, 13.676V, 0.290A, 231.196Ah, 3.97W",Subject: "Started DA_WCR_102723 phase initialPurge; da [1000, 1000] #da and wsr are run simultanelously" @17:27:15.62
Starting initialPurge @17:27:15.65 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @17:27:15.71 Hand.open @17:27:16.42 Elbow.reconfigure :tight @17:27:16.46 Elbow.seek FlushPuck::Garage @17:27:18.50 Elbow.reconfigure :loose @17:27:18.74 Forearm.reconfigure :loose @17:27:18.79 Forearm.seek :garage @17:27:19.79 Hand.close @17:27:19.85 upsync @17:27:20.53
Forearm.seek :retract @17:27:21.51 Elbow.reconfigure :tight @17:27:21.56 Elbow.seek CC @17:27:24.46 Elbow.reconfigure :loose @17:27:24.56 Forearm.reconfigure :tight @17:27:24.61 Forearm.seek CC @17:27:25.88 CC.seek :closed @17:27:30.42 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.32 @17:27:30.49 Hand.open @17:27:31.21 Forearm.reconfigure :loose @17:27:31.26 Forearm.seek :retract @17:27:32.29 Flushing the Collection Valve::Manifold @17:27:32.42 CSV.dial CSR @17:27:33.71 Pulling 4.999ml of CSR.flush @17:27:33.72 CSR.select :flush @17:27:33.84 CS.reconfigure :default @17:27:33.89 CS.seek 5ml @17:27:47.91 delay 5 seconds @17:27:52.93 CSR.select :air @17:27:53.10 CS.reconfigure :fast @17:27:53.15 CS.seek 7.5ml @17:27:59.88 CSV.dial :waste @17:28:01.17 CS.reconfigure :default @17:28:01.21 CS.seek 0ml @17:28:21.19 Purging external Intake @17:28:21.32 CSV.dial :flush @17:28:21.76 CS.reconfigure :fast @17:28:21.81 CS.seek 10ml @17:28:46.44 delay 5 seconds @17:28:51.46 CSV.dial CBV @17:28:51.50
CBV.dial CSV @17:28:52.24 CTV.dial :intake @17:28:53.03 Intake.select :open @17:28:53.15 CS.seek 0ml @17:29:16.63 Intake.select :close @17:29:16.74 CTV.dial :air @17:29:17.52 Flushing upper manifold @17:29:17.65 CSV.dial :flush @17:29:18.39 CS.seek 5ml @17:29:31.00 delay 5 seconds @17:29:36.03 CSV.dial :air @17:29:36.52 CS.seek 7ml @17:29:42.19 CSV.dial CBV @17:29:42.22
CBV.dial CSV @17:29:43.24 CTV.dial :air @17:29:43.26 CS.seek 0ml @17:29:59.82 delay 7 seconds @17:30:06.84 CS.seek 3ml @17:30:14.94 CSV.dial :waste @17:30:15.40 CS.seek 0ml @17:30:23.21 CSV.dial :flush @17:30:23.65 CS.seek 3ml @17:30:31.69 delay 5 seconds @17:30:36.71 CSV.dial :air @17:30:37.21 CS.seek 4.999ml @17:30:42.75 CSV.dial :air @17:30:42.84 CS.seek 0ml @17:30:54.94 delay 7 seconds @17:31:01.97 CS.seek 3ml @17:31:09.97 CSV.dial :waste @17:31:10.71 CS.seek 0ml @17:31:18.35 Garaging FlushPuck in Collection Clamp @17:31:18.42 Elbow.reconfigure :tight @17:31:18.46 Elbow.seek CC @17:31:18.73 Elbow.reconfigure :loose @17:31:18.83 Forearm.seek CC @17:31:19.85 Hand.close @17:31:20.52 CC.seek :open @17:31:25.59 Forearm.seek :retract @17:31:26.60 Elbow.reconfigure :tight @17:31:26.65 Elbow.seek :garage @17:31:29.58 Elbow.reconfigure :loose @17:31:29.68 Forearm.reconfigure :tight @17:31:29.73 Forearm.seek :garage @17:31:30.79 Hand.open @17:31:31.46 Forearm.reconfigure :loose @17:31:31.50 Forearm.seek :retract @17:31:32.52 da sampling at most 1000ml, wcr at most 1000ml @17:31:32.56 DAfans.email "Started da in tube 3 at 17:31:32UTC27-Oct-23\n{1 => 33,\n 2 => 15,\n 3 => 18,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@17:27:15, 25% flow, 20.4C, 21% humidity, 14.7psia, 13.676V, 0.290A, 231.196Ah, 3.97W",Subject: "da sampling at most 1000ml, wcr at most 1000ml" @17:31:32.60
Loading sh1 puck from tube 3 into Collection Clamp @17:31:32.86 SC.reconfigure :tight @17:31:32.92 SC.seek 3 @17:31:36.44 upsync @17:31:36.50
SC.configure :limp @17:31:36.74 Elbow.reconfigure :tight @17:31:36.79 Elbow.seek 3 @17:31:38.74 Forearm.reconfigure :tight @17:31:38.80 Forearm.seek 3 + 450 counts @17:31:39.84 SE.reconfigure :pick @17:31:39.90 SE.seek :beamBreak @17:31:41.97 SE.configure :default @17:31:42.14 Elbow.seek 3 @17:31:42.33 Forearm.seek 3 + 300 counts @17:31:42.55 Forearm.reconfigure :loose @17:31:42.60 Hand.close @17:31:43.28 SE.seek :down @17:31:43.50
Forearm.seek :retract @17:31:44.78 Elbow.seek CC @17:31:44.94 SE.stop @17:31:46.45
Elbow.reconfigure :loose @17:31:46.56 Forearm.reconfigure :tight @17:31:46.60 Forearm.seek CC @17:31:47.81 CC.seek :closed @17:31:52.35 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.24 @17:31:52.36 Hand.open @17:31:53.08 Forearm.reconfigure :loose @17:31:53.13 Forearm.seek :retract @17:31:54.10 collectSample 1000ml w/bubble point of 24psi @17:31:54.11 Priming 7ml @17:31:54.12 Intake.select :close @17:31:54.13 Exhaust.select :close @17:31:54.15 SS.hold @17:31:54.36 Calibrating pressure and temperature sensors @17:31:54.39 CTV.dial :air @17:31:54.40 SSV.dial :spit,avoiding: :exhaust @17:31:54.49 SS.reconfigure :fast @17:31:54.54 SS.seek 5ml @17:31:57.34 SSV.dial CBV @17:31:57.37
CBV.dial SSV @17:31:58.70 delay 3.8 seconds @17:32:02.63 Sensor limits: Intake max 108.6psia; Outlet max 102.5psia @17:32:02.64 SSV.dial :spit @17:32:03.97 SS.seek 0ml @17:32:06.73 SSV.dial CBV @17:32:08.05 CTV.dial :intake @17:32:08.83 SSV.dial CBV @17:32:08.86
CBV.dial SSV @17:32:08.94 Allowing 40s for 7.0ml of remaining 7.0ml @17:32:08.96 Intake.select :open @17:32:11.13 Intake @14.5psia, vacuum from 9.0 to 12.0psi @17:32:11.14 SS.reconfigure :inhaling 2.20ml/s @17:32:11.19 SS.seek 7ml @17:32:18.51 Stroke completed -- waiting for pressure to equalize ... @17:32:18.52 SS.reconfigure :exhale @17:32:18.57 SS.hold @17:32:24.37 Intake.select :close @17:32:24.53 SSV.dial :exhaust @17:32:25.28 Exhaust.select :open @17:32:25.39 SS.seek 0ml @17:32:29.36 Exhaust.select :close @17:32:29.47 Sampling 1000.0ml @17:32:29.48 Intake.select :close @17:32:29.49 Exhaust.select :close @17:32:29.50 SS.reconfigure :default @17:32:29.55 SS.hold @17:32:29.75 CTV.dial :intake @17:32:29.92 SSV.dial CBV @17:32:29.95
CBV.dial SSV @17:32:30.76 Allowing 130s for 25.0ml of remaining 1000.0ml @17:32:30.77 Intake.select :open @17:32:32.94 Intake @15.0psia, vacuum from 9.5 to 12.5psi @17:32:32.96 SS.reconfigure :inhaling 2.20ml/s @17:32:33.01 SS.seek 25ml @17:32:41.69 Vacuum too high -- waiting up to 40 seconds @17:32:41.71 SS.reconfigure :exhale @17:32:41.76 SS.hold @17:32:47.77 Intake @17.5psia, vacuum from 10.0 to 13.0psi @17:32:47.78 SS.reconfigure :inhaling 1.76ml/s @17:32:47.83 SS.seek 25ml @17:32:51.19 Vacuum too high -- waiting up to 40 seconds @17:32:51.20 SS.reconfigure :exhale @17:32:51.26 SS.hold @17:32:53.43 Intake @17.5psia, vacuum from 10.0 to 13.0psi @17:32:53.45 SS.reconfigure :inhaling 1.41ml/s @17:32:53.49 SS.seek 25ml @17:32:56.97 Vacuum too high -- waiting up to 40 seconds @17:32:56.99 SS.reconfigure :exhale @17:32:57.04 SS.hold @17:32:59.25 Intake @17.4psia, vacuum from 10.0 to 13.0psi @17:32:59.27 SS.reconfigure :inhaling 1.13ml/s @17:32:59.32 SS.seek 25ml @17:33:04.20 Stroke completed -- waiting for pressure to equalize ... @17:33:04.21 SS.reconfigure :exhale @17:33:04.25 SS.hold @17:33:15.38 Intake.select :close @17:33:15.91 SSV.dial :exhaust @17:33:16.68 Exhaust.select :open @17:33:16.80 SS.seek 0ml @17:33:25.87 Exhaust.select :close @17:33:25.98 SSV.dial CBV @17:33:26.00
CBV.dial SSV @17:33:26.78 Allowing 130s for 25.0ml of remaining 975.0ml @17:33:26.79 Intake.select :open @17:33:28.96 Intake @15.7psia, vacuum from 10.0 to 13.0psi @17:33:28.97 SS.reconfigure :inhaling 1.13ml/s @17:33:29.02 SS.seek 25ml @17:33:53.26 Stroke completed -- waiting for pressure to equalize ... @17:33:53.27 SS.reconfigure :exhale @17:33:53.32 SS.hold @17:34:11.66 Intake.select :close @17:34:11.82 SSV.dial :exhaust @17:34:12.59 Exhaust.select :open @17:34:12.70 SS.seek 0ml @17:34:21.78 Exhaust.select :close @17:34:21.89 SSV.dial CBV @17:34:21.91
CBV.dial SSV @17:34:22.70 Allowing 130s for 25.0ml of remaining 950.0ml @17:34:22.71 Intake.select :open @17:34:25.24 Intake @15.8psia, vacuum from 10.0 to 13.0psi @17:34:25.26 SS.reconfigure :inhaling 1.13ml/s @17:34:25.31 SS.seek 25ml @17:34:49.52 Stroke completed -- waiting for pressure to equalize ... @17:34:49.53 SS.reconfigure :exhale @17:34:49.58 SS.hold @17:35:07.93 Intake.select :close @17:35:08.09 SSV.dial :exhaust @17:35:08.84 Exhaust.select :open @17:35:08.95 SS.seek 0ml @17:35:18.09 Exhaust.select :close @17:35:18.20 SSV.dial CBV @17:35:18.23
CBV.dial SSV @17:35:18.98 Allowing 130s for 25.0ml of remaining 925.0ml @17:35:18.99 Intake.select :open @17:35:21.16 Intake @15.8psia, vacuum from 10.0 to 13.0psi @17:35:21.17 SS.reconfigure :inhaling 1.13ml/s @17:35:21.22 SS.seek 25ml @17:35:45.74 Stroke completed -- waiting for pressure to equalize ... @17:35:45.75 SS.reconfigure :exhale @17:35:45.80 SS.hold @17:36:04.34 Intake.select :close @17:36:04.49 SSV.dial :exhaust @17:36:05.26 Exhaust.select :open @17:36:05.37 SS.seek 0ml @17:36:14.37 Exhaust.select :close @17:36:14.48 SSV.dial CBV @17:36:14.50
CBV.dial SSV @17:36:15.29 Allowing 130s for 25.0ml of remaining 900.0ml @17:36:15.30 Intake.select :open @17:36:17.46 Intake @15.8psia, vacuum from 10.0 to 13.0psi @17:36:17.48 SS.reconfigure :inhaling 1.13ml/s @17:36:17.53 SS.seek 25ml @17:36:40.53 Vacuum too high -- waiting up to 40 seconds @17:36:40.54 SS.reconfigure :exhale @17:36:40.59 SS.hold @17:36:47.64 Intake @16.4psia, vacuum from 10.0 to 13.0psi @17:36:47.66 SS.reconfigure :inhaling 0.91ml/s @17:36:47.70 SS.seek 25ml @17:36:49.32 Stroke completed -- waiting for pressure to equalize ... @17:36:49.33 SS.reconfigure :exhale @17:36:49.37 SS.hold @17:36:59.19 Intake.select :close @17:36:59.34 SSV.dial :exhaust @17:37:00.10 Exhaust.select :open @17:37:00.21 SS.seek 0ml @17:37:09.01 Exhaust.select :close @17:37:09.12 SSV.dial CBV @17:37:09.14
CBV.dial SSV @17:37:09.94 Allowing 130s for 25.0ml of remaining 875.0ml @17:37:09.96 Intake.select :open @17:37:12.12 Intake @15.8psia, vacuum from 10.0 to 13.0psi @17:37:12.14 SS.reconfigure :inhaling 0.91ml/s @17:37:12.19 SS.seek 25ml @17:37:41.97 Stroke completed -- waiting for pressure to equalize ... @17:37:41.98 SS.reconfigure :exhale @17:37:42.03 SS.hold @17:37:55.08 Intake.select :close @17:37:55.24 SSV.dial :exhaust @17:37:56.01 Exhaust.select :open @17:37:56.12 SS.seek 0ml @17:38:05.25 Exhaust.select :close @17:38:05.37 SSV.dial CBV @17:38:05.40
CBV.dial SSV @17:38:06.15 Allowing 130s for 25.0ml of remaining 850.0ml @17:38:06.16 Intake.select :open @17:38:08.32 Intake @15.8psia, vacuum from 10.0 to 13.0psi @17:38:08.34 SS.reconfigure :inhaling 0.91ml/s @17:38:08.39 SS.seek 25ml @17:38:37.41 Stroke completed -- waiting for pressure to equalize ... @17:38:37.42 SS.reconfigure :exhale @17:38:37.46 SS.hold @17:38:51.84 Intake.select :close @17:38:51.99 SSV.dial :exhaust @17:38:52.75 Exhaust.select :open @17:38:52.87 SS.seek 0ml @17:39:01.89 Exhaust.select :close @17:39:02.01 SSV.dial CBV @17:39:02.03
CBV.dial SSV @17:39:02.82 Allowing 130s for 25.0ml of remaining 825.0ml @17:39:02.83 Intake.select :open @17:39:04.99 Intake @15.8psia, vacuum from 10.0 to 13.0psi @17:39:05.01 SS.reconfigure :inhaling 0.91ml/s @17:39:05.06 SS.seek 25ml @17:39:34.25 Stroke completed -- waiting for pressure to equalize ... @17:39:34.26 SS.reconfigure :exhale @17:39:34.31 SS.hold @17:39:50.96 Intake.select :close @17:39:51.12 SSV.dial :exhaust @17:39:51.88 Exhaust.select :open @17:39:51.99 SS.seek 0ml @17:40:00.78 Exhaust.select :close @17:40:00.89 SSV.dial CBV @17:40:00.92
CBV.dial SSV @17:40:01.71 Allowing 130s for 25.0ml of remaining 800.0ml @17:40:01.72 Intake.select :open @17:40:03.88 Intake @15.7psia, vacuum from 10.0 to 13.0psi @17:40:03.90 SS.reconfigure :inhaling 0.91ml/s @17:40:03.95 SS.seek 25ml @17:40:33.95 Stroke completed -- waiting for pressure to equalize ... @17:40:33.96 SS.reconfigure :exhale @17:40:34.01 SS.hold @17:40:52.35 Intake.select :close @17:40:52.50 SSV.dial :exhaust @17:40:53.27 Exhaust.select :open @17:40:53.38 SS.seek 0ml @17:41:02.14 Exhaust.select :close @17:41:02.25 SSV.dial CBV @17:41:02.28
CBV.dial SSV @17:41:03.06 Allowing 130s for 25.0ml of remaining 775.0ml @17:41:03.08 Intake.select :open @17:41:05.24 Intake @15.7psia, vacuum from 10.0 to 13.0psi @17:41:05.26 SS.reconfigure :inhaling 0.91ml/s @17:41:05.31 SS.seek 25ml @17:41:29.43 Vacuum too high -- waiting up to 40 seconds @17:41:29.44 SS.reconfigure :exhale @17:41:29.49 SS.hold @17:41:37.82 Intake @16.7psia, vacuum from 10.0 to 13.0psi @17:41:37.83 SS.reconfigure :inhaling 0.73ml/s @17:41:37.88 SS.seek 25ml @17:41:45.12 Stroke completed -- waiting for pressure to equalize ... @17:41:45.13 SS.reconfigure :exhale @17:41:45.18 SS.hold @17:41:56.23 Intake.select :close @17:41:56.39 SSV.dial :exhaust @17:41:57.14 Exhaust.select :open @17:41:57.25 SS.seek 0ml @17:42:06.08 Exhaust.select :close @17:42:06.19 SSV.dial CBV @17:42:06.23
CBV.dial SSV @17:42:06.97 Allowing 130s for 25.0ml of remaining 750.0ml @17:42:06.98 Intake.select :open @17:42:09.14 Intake @15.8psia, vacuum from 10.0 to 13.0psi @17:42:09.16 SS.reconfigure :inhaling 0.73ml/s @17:42:09.21 SS.seek 25ml @17:42:45.54 Stroke completed -- waiting for pressure to equalize ... @17:42:45.55 SS.reconfigure :exhale @17:42:45.60 SS.hold @17:43:02.94 Intake.select :close @17:43:03.10 SSV.dial :exhaust @17:43:03.86 Exhaust.select :open @17:43:03.97 SS.seek 0ml @17:43:12.76 Exhaust.select :close @17:43:12.88 SSV.dial CBV @17:43:12.90
CBV.dial SSV @17:43:13.67 Allowing 130s for 25.0ml of remaining 725.0ml @17:43:13.68 Intake.select :open @17:43:15.84 Intake @15.7psia, vacuum from 10.0 to 13.0psi @17:43:15.86 SS.reconfigure :inhaling 0.73ml/s @17:43:15.91 SS.seek 25ml @17:43:51.82 Stroke completed -- waiting for pressure to equalize ... @17:43:51.83 SS.reconfigure :exhale @17:43:51.88 SS.hold @17:44:10.22 Intake.select :close @17:44:10.73 SSV.dial :exhaust @17:44:11.56 Exhaust.select :open @17:44:11.67 SS.seek 0ml @17:44:20.44 Exhaust.select :close @17:44:20.55 SSV.dial CBV @17:44:20.58
CBV.dial SSV @17:44:21.34 Allowing 130s for 25.0ml of remaining 700.0ml @17:44:21.35 Intake.select :open @17:44:23.52 Intake @15.7psia, vacuum from 10.0 to 13.0psi @17:44:23.53 SS.reconfigure :inhaling 0.73ml/s @17:44:23.58 SS.seek 25ml @17:44:59.95 Stroke completed -- waiting for pressure to equalize ... @17:44:59.96 SS.reconfigure :exhale @17:45:00.00 SS.hold @17:45:20.41 Intake.select :close @17:45:20.56 SSV.dial :exhaust @17:45:21.33 Exhaust.select :open @17:45:21.44 SS.seek 0ml @17:45:30.43 Exhaust.select :close @17:45:30.54 SSV.dial CBV @17:45:30.57
CBV.dial SSV @17:45:31.34 Allowing 130s for 25.0ml of remaining 675.0ml @17:45:31.71 Intake.select :open @17:45:33.94 Intake @15.7psia, vacuum from 10.0 to 13.0psi @17:45:33.96 SS.reconfigure :inhaling 0.73ml/s @17:45:34.01 SS.seek 25ml @17:45:59.39 Vacuum too high -- waiting up to 40 seconds @17:45:59.41 SS.reconfigure :exhale @17:45:59.46 SS.hold @17:46:09.37 Intake @16.5psia, vacuum from 10.0 to 13.0psi @17:46:09.38 SS.reconfigure :inhaling 0.59ml/s @17:46:09.43 SS.seek 25ml @17:46:22.99 Stroke completed -- waiting for pressure to equalize ... @17:46:23.00 SS.reconfigure :exhale @17:46:23.05 SS.hold @17:46:37.77 Intake.select :close @17:46:37.92 SSV.dial :exhaust @17:46:38.68 Exhaust.select :open @17:46:38.80 SS.seek 0ml @17:46:47.59 Exhaust.select :close @17:46:47.70 SSV.dial CBV @17:46:47.74
CBV.dial SSV @17:46:48.48 Allowing 130s for 25.0ml of remaining 650.0ml @17:46:48.49 Intake.select :open @17:46:50.66 Intake @15.7psia, vacuum from 10.0 to 13.0psi @17:46:50.68 SS.reconfigure :inhaling 0.59ml/s @17:46:50.73 SS.seek 25ml @17:47:35.71 Stroke completed -- waiting for pressure to equalize ... @17:47:35.72 SS.reconfigure :exhale @17:47:35.77 SS.hold @17:47:56.24 Intake.select :close @17:47:56.39 SSV.dial :exhaust @17:47:57.16 Exhaust.select :open @17:47:57.27 SS.seek 0ml @17:48:06.46 Exhaust.select :close @17:48:06.57 SSV.dial CBV @17:48:06.60
CBV.dial SSV @17:48:07.35 Allowing 130s for 25.0ml of remaining 625.0ml @17:48:07.37 Intake.select :open @17:48:09.53 Intake @15.7psia, vacuum from 10.0 to 13.0psi @17:48:09.55 SS.reconfigure :inhaling 0.59ml/s @17:48:09.60 SS.seek 25ml @17:48:54.24 Stroke completed -- waiting for pressure to equalize ... @17:48:54.25 SS.reconfigure :exhale @17:48:54.30 SS.hold @17:49:18.79 Intake.select :close @17:49:18.95 SSV.dial :exhaust @17:49:19.71 Exhaust.select :open @17:49:19.82 SS.seek 0ml @17:49:28.58 Exhaust.select :close @17:49:28.70 SSV.dial CBV @17:49:28.74
CBV.dial SSV @17:49:29.49 Allowing 130s for 25.0ml of remaining 600.0ml @17:49:29.50 Intake.select :open @17:49:31.67 Intake @15.7psia, vacuum from 10.0 to 13.0psi @17:49:31.69 SS.reconfigure :inhaling 0.59ml/s @17:49:31.73 SS.seek 25ml @17:50:12.48 Vacuum too high -- waiting up to 40 seconds @17:50:12.50 SS.reconfigure :exhale @17:50:12.55 SS.hold @17:50:27.06 Intake @16.8psia, vacuum from 10.0 to 13.0psi @17:50:27.08 SS.reconfigure :inhaling 0.47ml/s @17:50:27.13 SS.seek 25ml @17:50:32.27 Stroke completed -- waiting for pressure to equalize ... @17:50:32.28 SS.reconfigure :exhale @17:50:32.33 SS.hold @17:50:48.87 Intake.select :close @17:50:49.03 SSV.dial :exhaust @17:50:49.79 Exhaust.select :open @17:50:49.90 SS.seek 0ml @17:50:58.86 Exhaust.select :close @17:50:58.98 SSV.dial CBV @17:50:59.00
CBV.dial SSV @17:50:59.79 Allowing 130s for 25.0ml of remaining 575.0ml @17:50:59.80 Intake.select :open @17:51:01.97 Intake @15.9psia, vacuum from 10.0 to 13.0psi @17:51:01.99 SS.reconfigure :inhaling 0.47ml/s @17:51:02.03 SS.seek 25ml @17:51:57.35 Stroke completed -- waiting for pressure to equalize ... @17:51:57.36 SS.reconfigure :exhale @17:51:57.40 SS.hold @17:52:22.33 Intake.select :close @17:52:22.49 SSV.dial :exhaust @17:52:23.25 Exhaust.select :open @17:52:23.37 SS.seek 0ml @17:52:32.36 Exhaust.select :close @17:52:32.47 SSV.dial CBV @17:52:32.50
CBV.dial SSV @17:52:33.27 Allowing 130s for 25.0ml of remaining 550.0ml @17:52:33.28 Intake.select :open @17:52:35.45 Intake @15.8psia, vacuum from 10.0 to 13.0psi @17:52:35.46 SS.reconfigure :inhaling 0.47ml/s @17:52:35.51 SS.seek 25ml @17:53:30.76 Stroke completed -- waiting for pressure to equalize ... @17:53:30.78 SS.reconfigure :exhale @17:53:30.82 SS.hold @17:53:59.59 Intake.select :close @17:53:59.75 SSV.dial :exhaust @17:54:00.53 Exhaust.select :open @17:54:00.65 SS.seek 0ml @17:54:09.53 Exhaust.select :close @17:54:09.64 SSV.dial CBV @17:54:09.66
CBV.dial SSV @17:54:10.45 Allowing 130s for 25.0ml of remaining 525.0ml @17:54:10.47 Intake.select :open @17:54:12.63 Intake @15.8psia, vacuum from 10.0 to 13.0psi @17:54:12.65 SS.reconfigure :inhaling 0.47ml/s @17:54:12.70 SS.seek 25ml @17:55:08.58 Stroke completed -- waiting for pressure to equalize ... @17:55:08.59 SS.reconfigure :exhale @17:55:08.63 SS.hold @17:55:41.50 Intake.select :close @17:55:41.66 SSV.dial :exhaust @17:55:42.43 Exhaust.select :open @17:55:42.54 SS.seek 0ml @17:55:51.34 Exhaust.select :close @17:55:51.45 SSV.dial CBV @17:55:51.48
CBV.dial SSV @17:55:52.25 Allowing 130s for 25.0ml of remaining 500.0ml @17:55:52.26 Intake.select :open @17:55:54.43 Intake @15.9psia, vacuum from 10.0 to 13.0psi @17:55:54.44 SS.reconfigure :inhaling 0.47ml/s @17:55:54.49 SS.seek 25ml @17:56:27.57 Vacuum too high -- waiting up to 40 seconds @17:56:27.58 SS.reconfigure :exhale @17:56:27.63 SS.hold @17:56:40.78 Intake @16.9psia, vacuum from 10.0 to 13.0psi @17:56:40.79 SS.reconfigure :inhaling 0.38ml/s @17:56:40.85 SS.seek 25ml @17:57:05.60 Vacuum too high -- waiting up to 40 seconds @17:57:05.61 SS.reconfigure :exhale @17:57:05.67 SS.hold @17:57:14.79 Intake @16.9psia, vacuum from 10.0 to 13.0psi @17:57:14.81 SS.reconfigure :inhaling 0.31ml/s @17:57:14.86 SS.seek 25ml @17:57:19.40 Stroke completed -- waiting for pressure to equalize ... @17:57:19.41 SS.reconfigure :exhale @17:57:19.46 SS.hold @17:57:35.68 Intake.select :close @17:57:35.84 SSV.dial :exhaust @17:57:36.61 Exhaust.select :open @17:57:36.72 SS.seek 0ml @17:57:45.53 Exhaust.select :close @17:57:45.64 SSV.dial CBV @17:57:45.67
CBV.dial SSV @17:57:46.43 Allowing 130s for 25.0ml of remaining 475.0ml @17:57:46.45 Intake.select :open @17:57:48.61 Intake @15.9psia, vacuum from 10.0 to 13.0psi @17:57:48.63 SS.reconfigure :inhaling 0.31ml/s @17:57:48.68 SS.seek 25ml @17:59:20.00 Stroke completed -- waiting for pressure to equalize ... @17:59:20.01 SS.reconfigure :exhale @17:59:20.06 SS.hold @17:59:44.49 Intake.select :close @17:59:44.64 SSV.dial :exhaust @17:59:45.42 Exhaust.select :open @17:59:45.53 SS.seek 0ml @17:59:54.57 Exhaust.select :close @17:59:54.68 SSV.dial CBV @17:59:54.70
CBV.dial SSV @17:59:55.48 Allowing 130s for 25.0ml of remaining 450.0ml @17:59:55.49 Intake.select :open @17:59:57.65 Intake @16.0psia, vacuum from 10.0 to 13.0psi @17:59:57.67 SS.reconfigure :inhaling 0.31ml/s @17:59:57.72 SS.seek 25ml @18:01:28.64 Stroke completed -- waiting for pressure to equalize ... @18:01:28.72 SS.reconfigure :exhale @18:01:28.77 SS.hold @18:01:59.24 Intake.select :close @18:01:59.39 SSV.dial :exhaust @18:02:00.16 Exhaust.select :open @18:02:00.28 SS.seek 0ml @18:02:09.18 Exhaust.select :close @18:02:09.29 SSV.dial CBV @18:02:09.32
CBV.dial SSV @18:02:10.42 Allowing 130s for 25.0ml of remaining 425.0ml @18:02:10.51 Intake.select :open @18:02:12.67 Intake @16.0psia, vacuum from 10.0 to 13.0psi @18:02:12.69 SS.reconfigure :inhaling 0.31ml/s @18:02:12.74 SS.seek 25ml @18:03:43.38 Stroke completed -- waiting for pressure to equalize ... @18:03:43.39 SS.reconfigure :exhale @18:03:43.44 SS.hold @18:04:16.12 Intake.select :close @18:04:16.27 SSV.dial :exhaust @18:04:17.11 Exhaust.select :open @18:04:17.24 SS.seek 0ml @18:04:26.07 Exhaust.select :close @18:04:26.18 SSV.dial CBV @18:04:26.21
CBV.dial SSV @18:04:26.97 Allowing 130s for 25.0ml of remaining 400.0ml @18:04:26.99 Intake.select :open @18:04:29.15 Intake @16.2psia, vacuum from 10.0 to 13.0psi @18:04:29.17 SS.reconfigure :inhaling 0.31ml/s @18:04:29.22 SS.seek 25ml @18:06:01.22 Stroke completed -- waiting for pressure to equalize ... @18:06:01.23 SS.reconfigure :exhale @18:06:01.27 SS.hold @18:06:37.00 SS.reconfigure :default @18:06:37.04 SS.hold @18:06:37.11 Intake.select :close @18:06:37.27 ShallowSampler::Clogged in MAIN -- Filtering too Slowly after 625.0ml @18:06:37.28 SSV.dial :exhaust @18:06:38.05 Exhaust.select :open @18:06:38.16 SS.reconfigure :exhale @18:06:38.21 SS.seek 0ml @18:06:47.26 Exhaust.select :close @18:06:47.38 Sampled 625.0ml @18:06:47.39 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @18:06:47.40 Intake.select :close @18:06:47.47 CSV.dial :waste @18:06:47.62 SSV.dial CBV,avoiding: :exhaust @18:06:47.65 CS.seek 0ml @18:06:47.70
CBV.dial SSV @18:06:47.84 CSV.dial :air @18:06:48.58 CS.seek 10ml @18:06:49.55
CTV.dial :air @18:06:50.32 delay 15 seconds @18:07:05.35 SSV.dial :spit,avoiding: :exhaust @18:07:06.66 CTV.dial CSV,avoiding: :intake @18:07:06.70
SS.seek 0ml @18:07:06.82 SSV.dial CBV,avoiding: :exhaust @18:07:13.15 SSV.dial between CBV and plug @18:07:13.65 CBV.dial between SSV and waste2 @18:07:14.04 CSV.dial between air and CTV @18:07:14.35 CS.seek 8.269ml @18:07:19.19 CTV.dial CSV,avoiding: :air @18:07:19.22
CSV.dial CTV @18:07:19.73 CS.reconfigure :pressurize @18:07:19.78 CS.seek 0ml @18:07:34.66 BubblePoint: Intake @24.2psia, Outlet @1.0psia @18:07:34.67 CBV.dial SSV @18:07:35.00 SS.reconfigure :default @18:07:35.05 SS.seek 10ml @18:07:40.60 delay 15 seconds @18:07:55.73 Evacuated: Intake @23.8psia, Outlet @23.3psia @18:07:55.74 SSV.dial :spit,avoiding: :exhaust @18:07:56.77 SS.reconfigure :exhale @18:07:56.82 SS.seek 0ml @18:08:01.84 SS.reconfigure :fast @18:08:01.95 SS.seek 5ml @18:08:04.79 SSV.dial CBV,avoiding: :exhaust @18:08:04.82
CBV.dial SSV @18:08:06.14 CTV.dial :air,avoiding: :intake @18:08:07.47 CSV.dial CTV @18:08:07.50
SS.seek 10ml @18:08:08.64 CSV.dial :air @18:08:09.08 CS.reconfigure :fast @18:08:09.12 CS.seek 0ml @18:08:10.02
SSV.dial :spit,avoiding: :exhaust @18:08:11.33 SS.reconfigure :exhale @18:08:11.38 SS.seek 0ml @18:08:16.24 CS.stop @18:08:16.60
SS.reconfigure :fast @18:08:16.65 SS.seek 5ml @18:08:19.46 SSV.dial CBV,avoiding: :exhaust @18:08:19.48
CBV.dial SSV @18:08:21.81 SSV.dial :spit,avoiding: :exhaust @18:08:23.14 SS.reconfigure :default @18:08:23.19 SS.seek 0ml @18:08:26.45 SS.stop @18:08:26.49 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @18:08:26.50 Intake.select :close @18:08:26.57 CSV.dial :waste @18:08:26.71 SSV.dial CBV,avoiding: :exhaust @18:08:26.74
CBV.dial SSV @18:08:27.34 CS.seek 0ml @18:08:27.40 CSV.dial :air @18:08:28.08
CTV.dial :air @18:08:28.17 delay 15 seconds @18:08:28.19 CS.seek 10ml @18:08:43.19
SSV.dial :spit,avoiding: :exhaust @18:08:44.52 CTV.dial CSV,avoiding: :intake @18:08:44.55
SS.reconfigure :exhale @18:08:44.66 SS.seek 0ml @18:08:45.09 SSV.dial CBV,avoiding: :exhaust @18:08:52.87 SSV.dial between CBV and plug @18:08:53.36 CBV.dial between SSV and waste2 @18:08:53.75 CSV.dial between air and CTV @18:08:54.06 CS.seek 7.225ml @18:09:01.22 CTV.dial CSV,avoiding: :air @18:09:01.24
CSV.dial CTV @18:09:01.61 CS.reconfigure :pressurize @18:09:01.66 CS.seek 0ml @18:09:16.71 BubblePoint: Intake @24.1psia, Outlet @3.7psia @18:09:16.72 CBV.dial SSV @18:09:17.04 SS.reconfigure :default @18:09:17.09 SS.seek 10ml @18:09:22.72 delay 15 seconds @18:09:37.85 Evacuated: Intake @23.9psia, Outlet @23.4psia @18:09:37.86 SSV.dial :spit,avoiding: :exhaust @18:09:38.89 SS.reconfigure :exhale @18:09:38.94 SS.seek 0ml @18:09:43.98 SS.reconfigure :fast @18:09:44.02 SS.seek 5ml @18:09:46.85 SSV.dial CBV,avoiding: :exhaust @18:09:46.87
CBV.dial SSV @18:09:48.23 CTV.dial :air,avoiding: :intake @18:09:49.57 CSV.dial CTV @18:09:49.61
SS.seek 10ml @18:09:50.82 CSV.dial :air @18:09:51.26 CS.reconfigure :fast @18:09:51.31 CS.seek 0ml @18:09:52.12
SSV.dial :spit,avoiding: :exhaust @18:09:53.44 SS.reconfigure :exhale @18:09:53.49 SS.seek 0ml @18:09:56.24 CS.stop @18:09:58.80
SS.reconfigure :fast @18:09:58.85 SS.seek 5ml @18:10:01.66 SSV.dial CBV,avoiding: :exhaust @18:10:01.69
CBV.dial SSV @18:10:04.08 SSV.dial :spit,avoiding: :exhaust @18:10:05.39 SS.reconfigure :default @18:10:05.44 SS.seek 0ml @18:10:08.67 SS.stop @18:10:08.71 Lysing: Adding 2.0ml AqMeOH @18:10:08.72 CBV.dial between CSV and waste1,avoiding: :DBV @18:10:10.21 CTV.dial CSV @18:10:11.52 CSV.dial CSR @18:10:12.31 Pulling 1ml of CSR.AqMeOH @18:10:12.32 CSR.select :AqMeOH @18:10:12.44 CS.reconfigure :default @18:10:12.49 CS.seek 1ml @18:10:15.90 delay 5 seconds @18:10:20.92 CSR.select :air @18:10:21.09 CS.seek 6.001ml @18:10:34.90 delay 2 seconds @18:10:36.91 CSV.dial :waste2 @18:10:37.35 CS.reconfigure :fast @18:10:37.41 CS.seek 0ml @18:10:51.74 CSV.dial CSR @18:10:52.19 Pulling 2ml of CSR.AqMeOH @18:10:52.20 CSR.select :AqMeOH @18:10:52.32 CS.reconfigure :default @18:10:52.37 CS.seek 2ml @18:10:58.74 delay 5 seconds @18:11:03.76 CSR.select :air @18:11:03.93 CS.seek 5ml @18:11:12.40 delay 2 seconds @18:11:14.41 CSV.dial CTV @18:11:14.86 CS.seek 1ml @18:11:26.03 Before lysing Collection Puck Heater at 18.4C/30.5psia @18:11:26.05 Heating Puck.from 3, :sh1 in Collection Clamp to 70C for 10:00 @18:11:26.07 CH.seek 70C,8:00 @18:13:36.36 CH.hold 10:00,70C @18:13:36.56 Begun lysing Collection Puck Heater at 68C/37.1psia @18:23:36.58 Ended lysing Collection Puck Heater at 70C/36.0psia @18:23:36.59 CS.seek 0ml @18:23:39.98 CTV.dial between spare and air,avoiding: :intake @18:23:41.01 CSV.dial :air @18:23:41.45 CS.seek 5ml @18:23:55.04 CSV.dial CTV @18:23:55.49 delay 2 seconds @18:23:57.51 CSV.dial :air @18:23:57.96 delay 2 seconds @18:23:59.98 CSV.dial CTV @18:24:00.44 delay 2 seconds @18:24:02.46 CSV.dial :air @18:24:02.90 delay 2 seconds @18:24:04.92 CS.seek 0ml @18:24:18.75 Puck @78.8psia after final lysis pressurization @18:24:18.76 Recovering Lysate into Collection syringe @18:24:18.77 CSV.dial CBV @18:24:19.78 CBV.dial CSV @18:24:20.15 CS.seek 5ml @18:24:33.73 delay 5 seconds @18:24:38.75 CTV.dial :air @18:24:39.29 CS.seek 7ml @18:24:45.23 delay 5 seconds @18:24:50.25 CSV.dial :air2 @18:24:50.70 CS.seek 10ml @18:24:59.23 CSV.dial between air and CTV,avoiding: CBV @18:25:00.50 Lysing Complete @18:25:00.56 Storing sh1 puck in Collection Clamp to tube 2 @18:25:00.74 SC.reconfigure :tight @18:25:00.79 SC.seek 2 @18:25:04.74 SC.configure :limp @18:25:04.96 Elbow.reconfigure :tight @18:25:05.01 Elbow.seek 2 @18:25:06.82 Forearm.reconfigure :tight @18:25:06.87 Forearm.seek 2 + 450 counts @18:25:07.93 SE.reconfigure :pick @18:25:07.99 SE.seek :beamBreak @18:25:10.09 SE.configure :default @18:25:10.25 Elbow.reconfigure :limp @18:25:10.30 Forearm.reconfigure :loose @18:25:10.35 Forearm.seek 2 @18:25:10.57 Elbow.hold @18:25:10.63 Elbow.reconfigure :tight @18:25:10.73 SE.seek 14.12 Pucks below Top Plate @18:25:11.21 Forearm.force -20 @18:25:11.25 Forearm.seek 2 + 306 counts @18:25:11.53 Forearm.reconfigure :tight @18:25:11.58 Forearm.seek 2 + 450 counts @18:25:11.81 SE.seek 15.12 Pucks below Top Plate @18:25:12.36 Forearm.reconfigure :loose @18:25:12.40 Forearm.seek :retract @18:25:13.34 Elbow.seek CC @18:25:15.04 Elbow.reconfigure :loose @18:25:15.14 Forearm.seek CC @18:25:16.14 Hand.close @18:25:16.73 CC.seek :open @18:25:21.46 Forearm.seek :retract @18:25:22.45 Elbow.reconfigure :tight @18:25:22.49 Elbow.seek 2 @18:25:24.69 Elbow.reconfigure :limp @18:25:24.75 Forearm.seek 2 @18:25:25.75 Elbow.hold @18:25:25.82 Elbow.reconfigure :tight @18:25:25.88 Hand.open @18:25:26.60 Forearm.force -20 @18:25:26.65 Forearm.seek 2 + 311 counts @18:25:27.05 Forearm.reconfigure :tight @18:25:27.10 Forearm.seek 2 + 450 counts @18:25:27.48 SE.seek :down @18:25:28.61
Forearm.reconfigure :loose @18:25:28.66 Forearm.seek :retract @18:25:29.15 SE.stop @18:25:29.59
Tranferring DA @18:25:29.60 Beginning samplePassC @18:25:29.61 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @18:25:29.81 Elbow.seek FlushPuck::Garage @18:25:31.60 Elbow.reconfigure :loose @18:25:31.71 Forearm.seek :garage @18:25:32.72 Hand.close @18:25:33.47 Forearm.seek :retract @18:25:34.40 Elbow.reconfigure :tight @18:25:34.45 Elbow.seek CC @18:25:37.42 Elbow.reconfigure :loose @18:25:37.52 Forearm.reconfigure :tight @18:25:37.57 Forearm.seek CC @18:25:39.26 CC.seek :closed @18:25:43.81 Collection Clamp onSpring/offSpring for 14/27 samples, on/off torque 2.25 @18:25:43.89 Hand.open @18:25:44.54 Forearm.reconfigure :loose @18:25:44.59 Forearm.seek :retract @18:25:45.54 PSV.dial PSR @18:25:47.04 Pulling 1.5ml of PSR.flush @18:25:47.05 PSR.select :flush @18:25:47.17 PS.seek 1.5ml @18:26:03.36 delay 5 seconds @18:26:08.38 PSV.dial :waste1 @18:26:08.86 PS.seek 0ml @18:26:25.01 PSV.dial PSR @18:26:25.48 PSR.select :air @18:26:25.60 PS.seek 2.5ml @18:26:51.89 PRV.dial CBV,avoiding: :ab2 @18:26:51.92
CBV.dial PRV @18:26:53.67 PSV.dial :mixloop @18:26:54.70 CTV.dial :air,avoiding: :intake @18:26:54.94 PTV.dial :PRVmixing @18:26:56.27 PS.seek 0ml @18:27:22.54 PSV.dial :air @18:27:23.13 PS.seek 2.5ml @18:27:49.48 PSV.dial :mixloop @18:27:49.94 PS.seek 0ml @18:28:16.26 PSV.dial :air @18:28:16.74 PS.seek 2.5ml @18:28:43.07 PSV.dial :mixloop @18:28:43.55 PS.seek 0ml @18:29:09.85 Garaging FlushPuck in Collection Clamp @18:29:09.92 Elbow.reconfigure :tight @18:29:10.02 Elbow.seek CC @18:29:10.53 Elbow.reconfigure :loose @18:29:11.08 Forearm.seek CC @18:29:12.11 Hand.close @18:29:12.72 CC.seek :open @18:29:17.34 Forearm.seek :retract @18:29:18.30 Elbow.reconfigure :tight @18:29:18.35 Elbow.seek :garage @18:29:21.23 Elbow.reconfigure :loose @18:29:21.33 Forearm.reconfigure :tight @18:29:21.38 Forearm.seek :garage @18:29:22.45 Hand.open @18:29:23.16 Forearm.reconfigure :loose @18:29:23.20 Forearm.seek :retract @18:29:24.24 PSV.dial :mixloop @18:29:24.31 PTV.dial :PRVmixing @18:29:24.32 PRV.dial CBV @18:29:24.35
CBV.dial PRV @18:29:24.58 CTV.dial :air,avoiding: :intake @18:29:24.60 Loading lyfil puck from tube 3 into Collection Clamp @18:29:24.83 SC.reconfigure :tight @18:29:24.88 SC.seek 3 @18:29:28.40 SC.configure :limp @18:29:28.62 Elbow.reconfigure :tight @18:29:28.67 Elbow.seek 3 @18:29:30.81 Forearm.reconfigure :tight @18:29:30.86 Forearm.seek 3 + 450 counts @18:29:31.89 SE.reconfigure :pick @18:29:31.95 SE.seek :beamBreak @18:29:33.99 SE.configure :default @18:29:34.15 Elbow.seek 3 @18:29:34.88 Forearm.seek 3 + 300 counts @18:29:35.07 Forearm.reconfigure :loose @18:29:35.11 Hand.close @18:29:35.73 SE.seek :down @18:29:35.94
Forearm.seek :retract @18:29:36.89 Elbow.seek CC @18:29:37.45 SE.stop @18:29:38.70
Elbow.reconfigure :loose @18:29:38.81 Forearm.reconfigure :tight @18:29:38.85 Forearm.seek CC @18:29:40.08 CC.seek :closed @18:29:44.60 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.16 @18:29:44.61 Hand.open @18:29:45.29 Forearm.reconfigure :loose @18:29:45.33 Forearm.seek :retract @18:29:46.30 CTV.dial CSV,avoiding: :intake @18:29:47.61 CSV.dial CTV @18:29:47.92 PS.seek 2.5ml @18:30:14.35 CS.seek 4.501ml @18:30:29.28 delay 8 seconds @18:30:37.31 PSV.dial :air @18:30:37.77 delay 2 seconds @18:30:39.79 PSV.dial :waste2 @18:30:40.53 PS.seek 0ml @18:31:06.91 CBV.dial between PRV and waste1 @18:31:07.22 CSV.dial :air @18:31:07.68 CS.seek 10ml @18:31:22.62 CSV.dial CTV @18:31:23.54 CS.seek 0ml @18:31:49.91 CBV.dial :waste1 @18:31:50.22 delay 5 seconds @18:31:55.24 CTV.dial :air,avoiding: :intake @18:31:56.64 Storing lyfil puck in Collection Clamp to tube 2 @18:31:56.82 SC.reconfigure :tight @18:31:56.87 SC.seek 2 @18:32:00.47 SC.configure :limp @18:32:00.69 Elbow.reconfigure :tight @18:32:00.73 Elbow.seek 2 @18:32:02.50 Forearm.reconfigure :tight @18:32:02.55 Forearm.seek 2 + 450 counts @18:32:03.59 SE.reconfigure :pick @18:32:03.65 SE.seek :beamBreak @18:32:05.76 SE.configure :default @18:32:05.92 Elbow.reconfigure :limp @18:32:05.97 Forearm.reconfigure :loose @18:32:06.02 Forearm.seek 2 @18:32:06.23 Elbow.hold @18:32:06.30 Elbow.reconfigure :tight @18:32:06.41 SE.seek 15.12 Pucks below Top Plate @18:32:06.87 Forearm.force -20 @18:32:06.91 Forearm.seek 2 + 306 counts @18:32:07.31 Forearm.reconfigure :tight @18:32:07.36 Forearm.seek 2 + 450 counts @18:32:07.66 SE.seek 16.12 Pucks below Top Plate @18:32:08.20 Forearm.reconfigure :loose @18:32:08.25 Forearm.seek :retract @18:32:09.58 Elbow.seek CC @18:32:11.30 Elbow.reconfigure :loose @18:32:11.41 Forearm.seek CC @18:32:12.34 Hand.close @18:32:12.97 CC.seek :open @18:32:17.71 Forearm.seek :retract @18:32:18.74 Elbow.reconfigure :tight @18:32:18.79 Elbow.seek 2 @18:32:20.55 Elbow.reconfigure :limp @18:32:20.60 Forearm.seek 2 @18:32:21.61 Elbow.hold @18:32:21.67 Elbow.reconfigure :tight @18:32:21.72 Hand.open @18:32:22.46 Forearm.force -20 @18:32:22.52 Forearm.seek 2 + 312 counts @18:32:22.90 Forearm.reconfigure :tight @18:32:22.95 Forearm.seek 2 + 450 counts @18:32:23.21 SE.seek :down @18:32:24.35
Forearm.reconfigure :loose @18:32:24.40 Forearm.seek :retract @18:32:24.83 SE.stop @18:32:25.33
PSV.dial :air @18:32:26.09 PS.seek 2.5ml @18:32:52.63 delay 1 second @18:32:53.65 PSV.dial :mixloop @18:32:54.10 delay 1 second @18:32:55.12 PSV.dial :air @18:32:55.58 delay 1 second @18:32:56.60 PSV.dial :mixloop @18:32:57.07 delay 1 second @18:32:58.08 PSV.dial :air @18:32:58.55 delay 1 second @18:32:59.64 PS.seek 0ml @18:33:26.01 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @18:33:26.25 Elbow.seek FlushPuck::Garage @18:33:28.01 Elbow.reconfigure :loose @18:33:28.11 Forearm.seek :garage @18:33:29.09 Hand.close @18:33:29.84 Forearm.seek :retract @18:33:30.81 Elbow.reconfigure :tight @18:33:30.86 Elbow.seek PC @18:33:32.15 Elbow.reconfigure :loose @18:33:32.26 Forearm.reconfigure :tight @18:33:32.31 Forearm.seek PC @18:33:33.55 PC.seek :closed @18:33:38.22 Processing Clamp onSpring/offSpring for 16/26 samples, on/off torque 1.59 @18:33:38.29 Hand.open @18:33:39.03 Forearm.reconfigure :loose @18:33:39.08 Forearm.seek :retract @18:33:40.03 PRV.dial :air @18:33:40.49 PSV.dial :mixloop @18:33:41.02 PS.seek 0.3ml @18:33:44.91 PSV.dial PSR @18:33:46.08 Pulling 0.4ml of PSR.air @18:33:46.09 PSR.select :air @18:33:46.11 PS.seek 0.7ml @18:33:51.10 delay 3 seconds @18:33:54.12 PSR.select :air @18:33:54.14 PS.seek 2.5ml @18:34:13.80 delay 3 seconds @18:34:16.82 PSV.dial :mixloop @18:34:17.85 PTV.dial :mixing @18:34:18.60 PBV.dial :waste2 @18:34:19.54 PS.seek 0ml @18:34:45.91 Garaging FlushPuck in Processing Clamp @18:34:45.99 Elbow.reconfigure :tight @18:34:46.03 Elbow.seek PC @18:34:46.16 Elbow.reconfigure :loose @18:34:46.26 Forearm.seek PC @18:34:47.20 Hand.close @18:34:47.84 PC.seek :open @18:34:52.56 Forearm.seek :retract @18:34:53.48 Elbow.reconfigure :tight @18:34:53.53 Elbow.seek :garage @18:34:54.90 Elbow.reconfigure :loose @18:34:55.00 Forearm.reconfigure :tight @18:34:55.05 Forearm.seek :garage @18:34:56.09 Hand.open @18:34:56.78 Forearm.reconfigure :loose @18:34:56.82 Forearm.seek :retract @18:34:57.79 Loading da puck from tube 3 into Processing Clamp @18:34:58.03 SC.reconfigure :tight @18:34:58.08 SC.seek 3 @18:35:01.61 SC.configure :limp @18:35:01.83 Elbow.reconfigure :tight @18:35:01.88 Elbow.seek 3 @18:35:03.78 Forearm.reconfigure :tight @18:35:03.82 Forearm.seek 3 + 450 counts @18:35:04.81 SE.reconfigure :pick @18:35:04.87 SE.seek :beamBreak @18:35:07.00 SE.configure :default @18:35:07.60 Elbow.seek 3 @18:35:07.81 Forearm.seek 3 + 300 counts @18:35:08.11 Forearm.reconfigure :loose @18:35:08.15 Hand.close @18:35:08.74 SE.seek :down @18:35:08.94
Forearm.seek :retract @18:35:09.91 Elbow.seek PC @18:35:10.54 SE.stop @18:35:12.43
Elbow.reconfigure :loose @18:35:12.53 Forearm.reconfigure :tight @18:35:12.58 Forearm.seek PC @18:35:13.79 PC.seek :closed @18:35:18.45 Processing Clamp onSpring/offSpring for 16/26 samples, on/off torque 1.62 @18:35:18.46 Hand.open @18:35:19.15 Forearm.reconfigure :loose @18:35:19.20 Forearm.seek :retract @18:35:20.21 Heating Puck.from 3, :da in Processing Clamp to 28C for 2:00:00 @18:35:20.22 PH.seek 28C,5:00 @18:35:51.43 PH.hold 2:00:00,28C @18:35:51.47 delay 1:30 @18:37:21.52 PSR.select :air @18:37:21.54 PSV.dial PSR @18:37:22.58 Pulling 0.5ml of PSR.wash @18:37:22.59 PSR.select :wash @18:37:22.71 PS.seek 0.5ml @18:37:28.59 delay 5 seconds @18:37:33.61 PSR.select :air @18:37:33.74 PS.seek 2ml @18:37:50.03 PTV.dial :puck @18:37:50.77 PSV.dial PTV @18:37:51.64 PBV.dial :waste1 @18:37:53.03 PS.seek 1.5ml @18:37:59.03 delay 3 seconds @18:38:02.05 PBV.dial between PSV and waste1 @18:38:02.40 PS.seek 0ml @18:38:18.53 delay 5:00 @18:43:18.56 recoverPSRreagent08 @18:43:18.57 PTV.dial :puck @18:43:18.65 PBV.dial :waste1 @18:43:19.01 delay 30 seconds @18:43:49.03 PBV.dial between PSV and waste1 @18:43:49.40 PSR.select :air @18:43:49.42 PSV.dial PSR @18:43:50.17 PS.reconfigure :fast @18:43:50.22 PS.seek 2.5ml @18:44:13.96 PSV.dial PTV @18:44:14.73 delay 5 seconds @18:44:19.75 PSV.dial PSR @18:44:20.49 delay 5 seconds @18:44:25.51 PSV.dial PTV @18:44:26.26 delay 5 seconds @18:44:31.28 PSV.dial :air @18:44:32.03 PS.seek 0ml @18:44:55.21 PTV.dial :PRVmixing,via: PRV @18:44:56.52 PSV.dial :mixloop @18:44:56.99 PRV.dial :ab1 @18:44:57.75 PS.reconfigure :slow @18:44:58.24 PS.seek 0.15ml @18:45:01.83 delay 2 seconds @18:45:03.85 CBV.dial PRV @18:45:03.88
PRV.dial CBV @18:45:04.45 PS.seek 0.35ml @18:45:09.08 delay 2 seconds @18:45:11.10 CBV.dial between PRV and PSV @18:45:11.41 PRV.dial :air @18:45:11.86 PS.seek 0.9ml @18:45:23.68 PRV.dial :waste2 @18:45:24.42 PS.seek 0.5ml @18:45:33.05 addPMLReagent08 @18:45:33.06 PSV.dial :air @18:45:33.53 PS.reconfigure :default @18:45:33.57 PS.seek 2ml @18:45:49.74 PSV.dial :PML @18:45:50.21 PTV.dial :mixing @18:45:50.96 PBV.dial :waste1 @18:45:51.38 PS.seek 1.85ml @18:45:53.80 PBV.dial between PSV and waste1 @18:45:54.18 PS.seek 0ml @18:46:13.83 delay 2 seconds @18:46:15.85 Beginning samplePassC @18:46:15.86 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @18:46:16.05 Elbow.reconfigure :tight @18:46:16.10 Elbow.seek FlushPuck::Garage @18:46:17.44 Elbow.reconfigure :loose @18:46:17.54 Forearm.seek :garage @18:46:18.57 Hand.close @18:46:19.33 Forearm.seek :retract @18:46:20.37 Elbow.reconfigure :tight @18:46:20.41 Elbow.seek CC @18:46:23.29 Elbow.reconfigure :loose @18:46:23.40 Forearm.reconfigure :tight @18:46:23.44 Forearm.seek CC @18:46:25.17 CC.seek :closed @18:46:29.70 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.34 @18:46:29.77 Hand.open @18:46:30.40 Forearm.reconfigure :loose @18:46:30.44 Forearm.seek :retract @18:46:31.39 PSV.dial PSR @18:46:32.44 Pulling 1.5ml of PSR.flush @18:46:32.45 PSR.select :flush @18:46:32.57 PS.seek 1.5ml @18:46:48.71 delay 5 seconds @18:46:53.73 PSV.dial :waste1 @18:46:54.21 PS.seek 0ml @18:47:10.36 PSV.dial PSR @18:47:10.83 PSR.select :air @18:47:10.95 PS.seek 2.5ml @18:47:37.34 PRV.dial CBV,avoiding: :ab2 @18:47:37.36
CBV.dial PRV @18:47:38.97 PSV.dial :mixloop @18:47:40.00 CTV.dial :air,avoiding: :intake @18:47:40.17 PTV.dial :PRVmixing @18:47:40.93 PS.seek 0ml @18:48:07.24 PSV.dial :air @18:48:07.71 PS.seek 2.5ml @18:48:34.20 PSV.dial :mixloop @18:48:34.68 PS.seek 0ml @18:49:01.05 PSV.dial :air @18:49:01.52 PS.seek 2.5ml @18:49:28.05 PSV.dial :mixloop @18:49:28.52 PS.seek 0ml @18:49:54.80 Garaging FlushPuck in Collection Clamp @18:49:54.87 Elbow.reconfigure :tight @18:49:54.92 Elbow.seek CC @18:49:55.18 Elbow.reconfigure :loose @18:49:55.74 Forearm.seek CC @18:49:56.81 Hand.close @18:49:57.46 CC.seek :open @18:50:02.07 Forearm.seek :retract @18:50:03.08 Elbow.reconfigure :tight @18:50:03.13 Elbow.seek :garage @18:50:05.99 Elbow.reconfigure :loose @18:50:06.10 Forearm.reconfigure :tight @18:50:06.14 Forearm.seek :garage @18:50:07.11 Hand.open @18:50:07.77 Forearm.reconfigure :loose @18:50:07.82 Forearm.seek :retract @18:50:08.78 delayUntil "18:54:15.84" @18:50:08.86 Whole Cell Archive sampling 1000ml @18:50:08.88 Loading wcr puck from tube 3 into Collection Clamp @18:50:09.17 SC.reconfigure :tight @18:50:09.23 SC.seek 3 @18:50:09.34 SC.configure :limp @18:50:09.57 Elbow.reconfigure :tight @18:50:09.62 Elbow.seek 3 @18:50:11.58 Forearm.reconfigure :tight @18:50:11.63 Forearm.seek 3 + 450 counts @18:50:12.71 SE.reconfigure :pick @18:50:12.77 SE.seek :beamBreak @18:50:14.97 SE.configure :default @18:50:15.14 Elbow.seek 3 @18:50:15.33 Forearm.seek 3 + 300 counts @18:50:15.85 Forearm.reconfigure :loose @18:50:15.90 Hand.close @18:50:16.47 SE.seek :down @18:50:16.68 Forearm.seek :retract @18:50:17.65 Elbow.seek CC @18:50:18.30 SE.stop @18:50:19.28 Elbow.reconfigure :loose @18:50:19.39 Forearm.reconfigure :tight @18:50:19.44 Forearm.seek CC @18:50:20.72 CC.seek :closed @18:50:25.25 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.28 @18:50:25.26 Hand.open @18:50:25.90 Forearm.reconfigure :loose @18:50:25.95 Forearm.seek :retract @18:50:26.92 collectSample 1000ml w/bubble point of 35psi @18:50:26.94 Priming 7ml @18:50:26.95 Intake.select :close @18:50:26.96 Exhaust.select :close @18:50:26.97 SS.hold @18:50:27.18 Calibrating pressure sensors @18:50:27.20 CTV.dial :air @18:50:27.21 SSV.dial :spit,avoiding: :exhaust @18:50:27.30 SS.reconfigure :fast @18:50:27.35 SS.seek 5ml @18:50:30.04 SSV.dial CBV @18:50:30.07 CBV.dial SSV @18:50:31.45 delay 3.8 seconds @18:50:35.39 Sensor limits: Intake max 108.7psia; Outlet max 102.2psia @18:50:35.40 SSV.dial :spit @18:50:36.75 SS.seek 0ml @18:50:39.54 SSV.dial CBV @18:50:40.88 CTV.dial :intake @18:50:41.66 SSV.dial CBV @18:50:41.69 CBV.dial SSV @18:50:41.84 Allowing 40s for 7.0ml of remaining 7.0ml @18:50:41.85 Intake.select :open @18:50:44.01 Intake @14.5psia, vacuum from 9.0 to 12.0psi @18:50:44.03 SS.reconfigure :inhaling 2.20ml/s @18:50:44.08 SS.seek 7ml @18:50:50.59 Stroke completed -- waiting for pressure to equalize ... @18:50:50.60 SS.reconfigure :exhale @18:50:50.65 SS.hold @18:51:01.81 Intake.select :close @18:51:01.97 SSV.dial :exhaust @18:51:02.76 Exhaust.select :open @18:51:02.88 SS.seek 0ml @18:51:07.49 Exhaust.select :close @18:51:07.61 Sampling 1000.0ml @18:51:07.62 Intake.select :close @18:51:07.63 Exhaust.select :close @18:51:07.65 SS.reconfigure :default @18:51:07.69 SS.hold @18:51:07.92 CTV.dial :intake @18:51:08.00 SSV.dial CBV @18:51:08.04 CBV.dial SSV @18:51:08.81 Allowing 130s for 25.0ml of remaining 1000.0ml @18:51:08.82 Intake.select :open @18:51:10.99 Intake @15.3psia, vacuum from 9.8 to 12.8psi @18:51:11.01 SS.reconfigure :inhaling 2.20ml/s @18:51:11.06 SS.seek 25ml @18:51:19.75 Vacuum too high -- waiting up to 40 seconds @18:51:19.77 SS.reconfigure :exhale @18:51:19.82 SS.hold @18:51:26.71 Intake @17.4psia, vacuum from 10.0 to 13.0psi @18:51:26.73 SS.reconfigure :inhaling 1.76ml/s @18:51:26.78 SS.seek 25ml @18:51:30.07 Vacuum too high -- waiting up to 40 seconds @18:51:30.09 SS.reconfigure :exhale @18:51:30.14 SS.hold @18:51:33.17 SS.reconfigure :inhaling 1.41ml/s @18:51:33.22 SS.seek 25ml @18:51:37.63 Vacuum too high -- waiting up to 40 seconds @18:51:37.65 SS.reconfigure :exhale @18:51:37.70 SS.hold @18:51:40.86 Intake @17.7psia, vacuum from 10.0 to 13.0psi @18:51:40.88 SS.reconfigure :inhaling 1.13ml/s @18:51:40.93 SS.seek 25ml @18:51:43.83 Stroke completed -- waiting for pressure to equalize ... @18:51:43.85 SS.reconfigure :exhale @18:51:43.90 SS.hold @18:52:08.53 Intake.select :close @18:52:08.70 SSV.dial :exhaust @18:52:09.46 Exhaust.select :open @18:52:09.58 SS.seek 0ml @18:52:18.38 Exhaust.select :close @18:52:18.50 SSV.dial CBV @18:52:18.54 CBV.dial SSV @18:52:19.29 Allowing 130s for 25.0ml of remaining 975.0ml @18:52:19.30 Intake.select :open @18:52:21.47 Intake @15.6psia, vacuum from 10.0 to 13.0psi @18:52:21.49 SS.reconfigure :inhaling 1.13ml/s @18:52:21.54 SS.seek 25ml @18:52:46.43 Stroke completed -- waiting for pressure to equalize ... @18:52:46.44 SS.reconfigure :exhale @18:52:46.49 SS.hold @18:53:17.47 Intake.select :close @18:53:17.63 SSV.dial :exhaust @18:53:18.40 Exhaust.select :open @18:53:18.51 SS.seek 0ml @18:53:27.79 Exhaust.select :close @18:53:27.98 SSV.dial CBV @18:53:28.00 CBV.dial SSV @18:53:28.77 Allowing 130s for 25.0ml of remaining 950.0ml @18:53:28.79 Intake.select :open @18:53:30.96 Intake @15.8psia, vacuum from 10.0 to 13.0psi @18:53:30.98 SS.reconfigure :inhaling 1.13ml/s @18:53:31.03 SS.seek 25ml @18:53:55.94 Stroke completed -- waiting for pressure to equalize ... @18:53:55.96 SS.reconfigure :exhale @18:53:56.01 SS.hold @18:54:15.85 recoverPRVreagent08 @18:54:15.86 PTV.dial :mixing @18:54:16.89 PBV.dial :waste1 @18:54:17.27 delay 30 seconds @18:54:23.10 Intake.select :close @18:54:23.26 SSV.dial :exhaust @18:54:24.02 Exhaust.select :open @18:54:24.14 SS.seek 0ml @18:54:33.16 Exhaust.select :close @18:54:33.28 SSV.dial CBV @18:54:33.31 CBV.dial SSV @18:54:34.05 Allowing 130s for 25.0ml of remaining 925.0ml @18:54:34.07 Intake.select :open @18:54:36.24 Intake @15.7psia, vacuum from 10.0 to 13.0psi @18:54:36.26 SS.reconfigure :inhaling 1.13ml/s @18:54:36.31 SS.seek 25ml @18:54:47.29 PBV.dial between PSV and waste1 @18:54:47.67 PSV.dial :air @18:54:48.14 PS.reconfigure :fast @18:54:48.19 PS.seek 2.5ml @18:55:00.58 Stroke completed -- waiting for pressure to equalize ... @18:55:00.59 SS.reconfigure :exhale @18:55:00.64 SS.hold @18:55:12.29 PSV.dial :mixloop @18:55:12.76 delay 2 seconds @18:55:14.78 PSV.dial :air @18:55:15.25 delay 2 seconds @18:55:17.26 PSV.dial :mixloop @18:55:17.74 delay 2 seconds @18:55:19.76 PSV.dial :air @18:55:20.23 PS.seek 0ml @18:55:23.57 Intake.select :close @18:55:23.73 SSV.dial :exhaust @18:55:24.51 Exhaust.select :open @18:55:24.62 SS.seek 0ml @18:55:33.64 Exhaust.select :close @18:55:33.76 SSV.dial CBV @18:55:33.80 CBV.dial SSV @18:55:34.55 Allowing 130s for 25.0ml of remaining 900.0ml @18:55:34.57 Intake.select :open @18:55:36.73 Intake @16.0psia, vacuum from 10.0 to 13.0psi @18:55:36.75 SS.reconfigure :inhaling 1.13ml/s @18:55:36.80 SS.seek 25ml @18:55:44.01 washCycleDA #1 of 2 @18:55:44.02 PSR.select :air @18:55:44.04 PSV.dial PSR @18:55:45.37 Pulling 0.5ml of PSR.wash @18:55:45.38 PSR.select :wash @18:55:45.50 PS.reconfigure :default @18:55:45.55 PS.seek 0.5ml @18:55:51.51 delay 5 seconds @18:55:56.53 PSR.select :air @18:55:56.66 PS.seek 2ml @18:55:58.31 Vacuum too high -- waiting up to 40 seconds @18:55:58.33 SS.reconfigure :exhale @18:55:58.38 SS.hold @18:56:05.33 Intake @17.2psia, vacuum from 10.0 to 13.0psi @18:56:05.35 SS.reconfigure :inhaling 0.91ml/s @18:56:05.40 SS.seek 25ml @18:56:09.50 Stroke completed -- waiting for pressure to equalize ... @18:56:09.51 SS.reconfigure :exhale @18:56:09.56 SS.hold @18:56:12.82 PTV.dial :puck @18:56:13.57 PSV.dial PTV @18:56:14.32 PBV.dial :waste1 @18:56:15.04 PS.seek 1.5ml @18:56:21.07 delay 3 seconds @18:56:24.09 PBV.dial between PSV and waste1 @18:56:24.45 PS.seek 0ml @18:56:25.99 Intake.select :close @18:56:26.15 SSV.dial :exhaust @18:56:26.93 Exhaust.select :open @18:56:27.04 SS.seek 0ml @18:56:35.85 Exhaust.select :close @18:56:35.96 SSV.dial CBV @18:56:35.99 CBV.dial SSV @18:56:36.76 Allowing 130s for 25.0ml of remaining 875.0ml @18:56:36.77 Intake.select :open @18:56:38.94 Intake @15.8psia, vacuum from 10.0 to 13.0psi @18:56:38.96 SS.reconfigure :inhaling 0.91ml/s @18:56:39.01 SS.seek 25ml @18:56:40.79 delay 2:00 @18:57:09.02 Stroke completed -- waiting for pressure to equalize ... @18:57:09.03 SS.reconfigure :exhale @18:57:09.08 SS.hold @18:57:29.64 Intake.select :close @18:57:29.80 SSV.dial :exhaust @18:57:30.57 Exhaust.select :open @18:57:30.69 SS.seek 0ml @18:57:39.39 Exhaust.select :close @18:57:39.51 SSV.dial CBV @18:57:39.53 CBV.dial SSV @18:57:40.30 Allowing 130s for 25.0ml of remaining 850.0ml @18:57:40.31 Intake.select :open @18:57:42.48 Intake @15.7psia, vacuum from 10.0 to 13.0psi @18:57:42.50 SS.reconfigure :inhaling 0.91ml/s @18:57:42.55 SS.seek 25ml @18:58:12.62 Stroke completed -- waiting for pressure to equalize ... @18:58:12.64 SS.reconfigure :exhale @18:58:12.69 SS.hold @18:58:31.52 Intake.select :close @18:58:31.68 SSV.dial :exhaust @18:58:32.45 Exhaust.select :open @18:58:32.57 SS.seek 0ml @18:58:40.81 recoverPSRreagent08 @18:58:40.82 PTV.dial :puck @18:58:40.90 PBV.dial :waste1 @18:58:41.26 delay 30 seconds @18:58:41.38 Exhaust.select :close @18:58:41.49 SSV.dial CBV @18:58:41.52 CBV.dial SSV @18:58:42.30 Allowing 130s for 25.0ml of remaining 825.0ml @18:58:42.31 Intake.select :open @18:58:44.48 Intake @15.8psia, vacuum from 10.0 to 13.0psi @18:58:44.50 SS.reconfigure :inhaling 0.91ml/s @18:58:44.55 SS.seek 25ml @18:59:11.28 PBV.dial between PSV and waste1 @18:59:11.64 PSR.select :air @18:59:12.01 PSV.dial PSR @18:59:12.89 PS.reconfigure :fast @18:59:12.94 PS.seek 2.5ml @18:59:14.53 Stroke completed -- waiting for pressure to equalize ... @18:59:14.54 SS.reconfigure :exhale @18:59:14.59 SS.hold @18:59:35.02 Intake.select :close @18:59:35.19 SSV.dial :exhaust @18:59:35.95 Exhaust.select :open @18:59:36.07 SS.seek 0ml @18:59:37.07 PSV.dial PTV @18:59:37.83 delay 5 seconds @18:59:42.85 PSV.dial PSR @18:59:43.61 delay 5 seconds @18:59:45.72 Exhaust.select :close @18:59:45.83 SSV.dial CBV @18:59:45.87 CBV.dial SSV @18:59:46.63 Allowing 130s for 25.0ml of remaining 800.0ml @18:59:46.64 Intake.select :open @18:59:48.63 PSV.dial PTV @18:59:48.80 Intake @15.7psia, vacuum from 10.0 to 13.0psi @18:59:48.82 SS.reconfigure :inhaling 0.91ml/s @18:59:48.87 SS.seek 25ml @18:59:49.41 delay 5 seconds @18:59:54.43 PSV.dial :air @18:59:55.20 PS.seek 0ml @19:00:17.73 Stroke completed -- waiting for pressure to equalize ... @19:00:17.74 SS.reconfigure :exhale @19:00:17.79 SS.hold @19:00:18.74 washCycleDA #2 of 2 @19:00:18.75 PSR.select :air @19:00:18.77 PSV.dial PSR @19:00:20.10 Pulling 0.5ml of PSR.wash @19:00:20.11 PSR.select :wash @19:00:20.30 PS.reconfigure :default @19:00:20.35 PS.seek 0.5ml @19:00:26.22 delay 5 seconds @19:00:31.24 PSR.select :air @19:00:31.36 PS.seek 2ml @19:00:36.67 Intake.select :close @19:00:36.83 SSV.dial :exhaust @19:00:37.59 Exhaust.select :open @19:00:37.71 SS.seek 0ml @19:00:46.73 Exhaust.select :close @19:00:46.85 SSV.dial CBV @19:00:46.87 CBV.dial SSV @19:00:47.56 PTV.dial :puck @19:00:47.66 PSV.dial PTV @19:00:47.68 Allowing 130s for 25.0ml of remaining 775.0ml @19:00:47.71 Intake.select :open @19:00:48.46 PBV.dial :waste1 @19:00:48.89 PS.seek 1.5ml @19:00:49.92 Intake @15.6psia, vacuum from 10.0 to 13.0psi @19:00:49.94 SS.reconfigure :inhaling 0.91ml/s @19:00:49.99 SS.seek 25ml @19:00:54.88 delay 3 seconds @19:00:57.90 PBV.dial between PSV and waste1 @19:00:58.28 PS.seek 0ml @19:01:14.41 delay 2:00 @19:01:19.34 Stroke completed -- waiting for pressure to equalize ... @19:01:19.35 SS.reconfigure :exhale @19:01:19.40 SS.hold @19:01:39.85 Intake.select :close @19:01:40.01 SSV.dial :exhaust @19:01:40.80 Exhaust.select :open @19:01:40.92 SS.seek 0ml @19:01:50.03 Exhaust.select :close @19:01:50.14 SSV.dial CBV @19:01:50.17 CBV.dial SSV @19:01:50.94 Allowing 130s for 25.0ml of remaining 750.0ml @19:01:50.95 Intake.select :open @19:01:53.12 Intake @15.5psia, vacuum from 10.0 to 13.0psi @19:01:53.14 SS.reconfigure :inhaling 0.91ml/s @19:01:53.19 SS.seek 25ml @19:02:22.76 Stroke completed -- waiting for pressure to equalize ... @19:02:22.77 SS.reconfigure :exhale @19:02:22.82 SS.hold @19:02:45.75 Intake.select :close @19:02:45.91 SSV.dial :exhaust @19:02:46.68 Exhaust.select :open @19:02:46.80 SS.seek 0ml @19:02:55.65 Exhaust.select :close @19:02:55.76 SSV.dial CBV @19:02:55.79 CBV.dial SSV @19:02:56.56 Allowing 130s for 25.0ml of remaining 725.0ml @19:02:56.57 Intake.select :open @19:02:58.74 Intake @15.7psia, vacuum from 10.0 to 13.0psi @19:02:58.76 SS.reconfigure :inhaling 0.91ml/s @19:02:58.81 SS.seek 25ml @19:03:14.43 recoverPSRreagent08 @19:03:14.44 PTV.dial :puck @19:03:14.46 PBV.dial :waste1 @19:03:14.83 delay 30 seconds @19:03:22.05 Vacuum too high -- waiting up to 40 seconds @19:03:22.53 SS.reconfigure :exhale @19:03:22.58 SS.hold @19:03:31.26 Intake @15.8psia, vacuum from 10.0 to 13.0psi @19:03:31.28 SS.reconfigure :inhaling 0.73ml/s @19:03:31.33 SS.seek 25ml @19:03:39.76 Stroke completed -- waiting for pressure to equalize ... @19:03:39.77 SS.reconfigure :exhale @19:03:39.82 SS.hold @19:03:44.85 PBV.dial between PSV and waste1 @19:03:45.23 PSR.select :air @19:03:45.25 PSV.dial PSR @19:03:46.02 PS.reconfigure :fast @19:03:46.07 PS.seek 2.5ml @19:03:54.70 Intake.select :close @19:03:54.86 SSV.dial :exhaust @19:03:55.63 Exhaust.select :open @19:03:55.75 SS.seek 0ml @19:04:04.96 Exhaust.select :close @19:04:05.08 SSV.dial CBV @19:04:05.11 CBV.dial SSV @19:04:05.87 Allowing 130s for 25.0ml of remaining 700.0ml @19:04:05.88 Intake.select :open @19:04:08.05 Intake @15.6psia, vacuum from 10.0 to 13.0psi @19:04:08.07 SS.reconfigure :inhaling 0.73ml/s @19:04:08.12 SS.seek 25ml @19:04:10.00 PSV.dial PTV @19:04:10.77 delay 5 seconds @19:04:15.79 PSV.dial PSR @19:04:16.53 delay 5 seconds @19:04:21.55 PSV.dial PTV @19:04:22.31 delay 5 seconds @19:04:27.33 PSV.dial :air @19:04:28.08 PS.seek 0ml @19:04:42.55 Vacuum too high -- waiting up to 40 seconds @19:04:42.56 SS.reconfigure :exhale @19:04:42.62 SS.hold @19:04:51.58 getPMLReagent08 @19:04:51.59 PRV.dial :air @19:04:52.06 delay 5 seconds @19:04:52.84 Intake @15.8psia, vacuum from 10.0 to 13.0psi @19:04:52.86 SS.reconfigure :inhaling 0.59ml/s @19:04:52.91 SS.seek 25ml @19:04:54.33 Stroke completed -- waiting for pressure to equalize ... @19:04:54.35 SS.reconfigure :exhale @19:04:54.40 SS.hold @19:04:57.08 PTV.dial :PRVmixing @19:04:58.44 delay 5 seconds @19:05:03.46 PRV.dial :ab2 @19:05:03.90 PTV.dial :PRVmixing @19:05:03.98 PSV.dial :mixloop @19:05:04.45 PS.reconfigure :default @19:05:04.50 PS.seek 0.3ml @19:05:07.10 Intake.select :close @19:05:07.27 SSV.dial :exhaust @19:05:08.06 Exhaust.select :open @19:05:08.17 SS.seek 0ml @19:05:08.40 delay 2 seconds @19:05:10.42 PRV.dial :air @19:05:10.95 PS.seek 0.55ml @19:05:14.40 addPMLReagent08 @19:05:14.42 PSV.dial :air @19:05:14.89 PS.seek 2ml @19:05:17.16 Exhaust.select :close @19:05:17.28 SSV.dial CBV @19:05:17.32 CBV.dial SSV @19:05:18.07 Allowing 130s for 25.0ml of remaining 675.0ml @19:05:18.08 Intake.select :open @19:05:20.25 Intake @15.7psia, vacuum from 10.0 to 13.0psi @19:05:20.27 SS.reconfigure :inhaling 0.59ml/s @19:05:20.32 SS.seek 25ml @19:05:30.53 PSV.dial :PML @19:05:31.00 PTV.dial :mixing @19:05:31.75 PBV.dial :waste1 @19:05:32.18 PS.seek 1.85ml @19:05:34.62 PBV.dial between PSV and waste1 @19:05:35.00 PS.seek 0ml @19:05:54.75 delay 2 seconds @19:05:56.77 delay 8:00 @19:06:04.69 Stroke completed -- waiting for pressure to equalize ... @19:06:04.71 SS.reconfigure :exhale @19:06:04.75 SS.hold @19:06:23.58 Intake.select :close @19:06:23.74 SSV.dial :exhaust @19:06:24.51 Exhaust.select :open @19:06:24.62 SS.seek 0ml @19:06:33.63 Exhaust.select :close @19:06:33.74 SSV.dial CBV @19:06:33.78 CBV.dial SSV @19:06:34.52 Allowing 130s for 25.0ml of remaining 650.0ml @19:06:34.54 Intake.select :open @19:06:37.11 Intake @15.7psia, vacuum from 10.0 to 13.0psi @19:06:37.20 SS.reconfigure :inhaling 0.59ml/s @19:06:37.25 SS.seek 25ml @19:07:21.63 Stroke completed -- waiting for pressure to equalize ... @19:07:21.64 SS.reconfigure :exhale @19:07:21.69 SS.hold @19:07:44.16 Intake.select :close @19:07:44.32 SSV.dial :exhaust @19:07:45.50 Exhaust.select :open @19:07:45.68 SS.seek 0ml @19:07:54.74 Exhaust.select :close @19:07:54.85 SSV.dial CBV @19:07:54.88 CBV.dial SSV @19:07:55.65 Allowing 130s for 25.0ml of remaining 625.0ml @19:07:55.66 Intake.select :open @19:07:57.82 Intake @15.7psia, vacuum from 10.0 to 13.0psi @19:07:57.84 SS.reconfigure :inhaling 0.59ml/s @19:07:57.89 SS.seek 25ml @19:08:33.42 Vacuum too high -- waiting up to 40 seconds @19:08:33.43 SS.reconfigure :exhale @19:08:33.49 SS.hold @19:08:44.76 Intake @15.9psia, vacuum from 10.0 to 13.0psi @19:08:44.78 SS.reconfigure :inhaling 0.47ml/s @19:08:44.83 SS.seek 25ml @19:08:56.16 Stroke completed -- waiting for pressure to equalize ... @19:08:56.17 SS.reconfigure :exhale @19:08:56.22 SS.hold @19:09:12.88 Intake.select :close @19:09:13.05 SSV.dial :exhaust @19:09:13.83 Exhaust.select :open @19:09:13.95 SS.seek 0ml @19:09:22.67 Exhaust.select :close @19:09:22.79 SSV.dial CBV @19:09:22.83 CBV.dial SSV @19:09:23.58 Allowing 130s for 25.0ml of remaining 600.0ml @19:09:23.60 Intake.select :open @19:09:25.77 Intake @15.5psia, vacuum from 10.0 to 13.0psi @19:09:25.79 SS.reconfigure :inhaling 0.47ml/s @19:09:25.84 SS.seek 25ml @19:10:21.39 Stroke completed -- waiting for pressure to equalize ... @19:10:21.40 SS.reconfigure :exhale @19:10:21.89 SS.hold @19:10:46.44 Intake.select :close @19:10:46.60 SSV.dial :exhaust @19:10:47.38 Exhaust.select :open @19:10:47.49 SS.seek 0ml @19:10:56.95 Exhaust.select :close @19:10:57.07 SSV.dial CBV @19:10:57.09 CBV.dial SSV @19:10:57.86 Allowing 130s for 25.0ml of remaining 575.0ml @19:10:57.88 Intake.select :open @19:11:00.05 Intake @15.7psia, vacuum from 10.0 to 13.0psi @19:11:00.07 SS.reconfigure :inhaling 0.47ml/s @19:11:00.12 SS.seek 25ml @19:11:55.45 Stroke completed -- waiting for pressure to equalize ... @19:11:55.46 SS.reconfigure :exhale @19:11:55.51 SS.hold @19:12:26.64 Intake.select :close @19:12:26.81 SSV.dial :exhaust @19:12:27.58 Exhaust.select :open @19:12:27.70 SS.seek 0ml @19:12:36.70 Exhaust.select :close @19:12:36.82 SSV.dial CBV @19:12:36.85 CBV.dial SSV @19:12:37.61 Allowing 130s for 25.0ml of remaining 550.0ml @19:12:37.62 Intake.select :open @19:12:39.79 Intake @15.6psia, vacuum from 10.0 to 13.0psi @19:12:39.81 SS.reconfigure :inhaling 0.47ml/s @19:12:39.86 SS.seek 25ml @19:13:34.99 Stroke completed -- waiting for pressure to equalize ... @19:13:35.01 SS.reconfigure :exhale @19:13:35.06 SS.hold @19:13:56.78 recoverPRVreagent08 @19:13:56.80 PTV.dial :mixing @19:13:56.88 PBV.dial :waste1 @19:13:57.25 delay 30 seconds @19:14:11.98 Intake.select :close @19:14:12.14 SSV.dial :exhaust @19:14:12.92 Exhaust.select :open @19:14:13.04 SS.seek 0ml @19:14:21.93 Exhaust.select :close @19:14:22.05 SSV.dial CBV @19:14:22.09 CBV.dial SSV @19:14:22.84 Allowing 130s for 25.0ml of remaining 525.0ml @19:14:22.86 Intake.select :open @19:14:25.02 Intake @15.6psia, vacuum from 10.0 to 13.0psi @19:14:25.04 SS.reconfigure :inhaling 0.47ml/s @19:14:25.09 SS.seek 25ml @19:14:27.27 PBV.dial between PSV and waste1 @19:14:27.63 PSV.dial :air @19:14:28.10 PS.reconfigure :fast @19:14:28.15 PS.seek 2.5ml @19:14:52.77 PSV.dial :mixloop @19:14:53.24 delay 2 seconds @19:14:55.25 PSV.dial :air @19:14:55.72 delay 2 seconds @19:14:57.74 PSV.dial :mixloop @19:14:58.22 delay 2 seconds @19:15:00.24 PSV.dial :air @19:15:00.71 PS.seek 0ml @19:15:08.91 Vacuum too high -- waiting up to 40 seconds @19:15:08.93 SS.reconfigure :exhale @19:15:08.98 SS.hold @19:15:24.20 washCycleDA #1 of 3 @19:15:24.21 PSR.select :air @19:15:24.23 PSV.dial PSR @19:15:25.56 Pulling 0.5ml of PSR.wash @19:15:25.57 PSR.select :wash @19:15:25.69 PS.reconfigure :default @19:15:25.74 PS.seek 0.5ml @19:15:29.57 Intake @16.3psia, vacuum from 10.0 to 13.0psi @19:15:29.59 SS.reconfigure :inhaling 0.38ml/s @19:15:29.64 SS.seek 25ml @19:15:32.23 delay 5 seconds @19:15:37.25 PSR.select :air @19:15:37.38 PS.seek 2ml @19:15:44.60 Stroke completed -- waiting for pressure to equalize ... @19:15:44.62 SS.reconfigure :exhale @19:15:44.67 SS.hold @19:15:53.54 PTV.dial :puck @19:15:54.36 PSV.dial PTV @19:15:55.12 PBV.dial :waste1 @19:15:55.53 PS.seek 1.5ml @19:16:01.58 delay 3 seconds @19:16:04.59 PBV.dial between PSV and waste1 @19:16:04.96 PS.seek 0ml @19:16:09.54 Intake.select :close @19:16:09.70 SSV.dial :exhaust @19:16:10.47 Exhaust.select :open @19:16:10.58 SS.seek 0ml @19:16:19.85 Exhaust.select :close @19:16:19.97 SSV.dial CBV @19:16:19.99 CBV.dial SSV @19:16:20.76 Allowing 130s for 25.0ml of remaining 500.0ml @19:16:20.77 Intake.select :open @19:16:21.05 delay 2:00 @19:16:22.95 Intake @15.5psia, vacuum from 10.0 to 13.0psi @19:16:22.97 SS.reconfigure :inhaling 0.38ml/s @19:16:23.02 SS.seek 25ml @19:17:33.38 Stroke completed -- waiting for pressure to equalize ... @19:17:33.39 SS.reconfigure :exhale @19:17:33.44 SS.hold @19:18:12.90 Intake.select :close @19:18:13.07 SSV.dial :exhaust @19:18:13.84 Exhaust.select :open @19:18:13.96 SS.seek 0ml @19:18:21.07 recoverPSRreagent08 @19:18:21.08 PTV.dial :puck @19:18:21.15 PBV.dial :waste1 @19:18:21.51 delay 30 seconds @19:18:23.01 Exhaust.select :close @19:18:23.12 SSV.dial CBV @19:18:23.15 CBV.dial SSV @19:18:23.91 Allowing 130s for 25.0ml of remaining 475.0ml @19:18:23.93 Intake.select :open @19:18:26.10 Intake @15.6psia, vacuum from 10.0 to 13.0psi @19:18:26.53 SS.reconfigure :inhaling 0.38ml/s @19:18:26.64 SS.seek 25ml @19:18:51.53 PBV.dial between PSV and waste1 @19:18:51.89 PSR.select :air @19:18:51.91 PSV.dial PSR @19:18:52.67 PS.reconfigure :fast @19:18:52.72 PS.seek 2.5ml @19:19:16.82 PSV.dial PTV @19:19:17.59 delay 5 seconds @19:19:22.61 PSV.dial PSR @19:19:23.37 delay 5 seconds @19:19:24.22 Vacuum too high -- waiting up to 40 seconds @19:19:24.23 SS.reconfigure :exhale @19:19:24.29 SS.hold @19:19:28.39 PSV.dial PTV @19:19:29.15 delay 5 seconds @19:19:34.22 PSV.dial :air @19:19:35.09 PS.seek 0ml @19:19:47.05 Intake @16.7psia, vacuum from 10.0 to 13.0psi @19:19:47.07 SS.reconfigure :inhaling 0.31ml/s @19:19:47.12 SS.seek 25ml @19:19:58.42 washCycleDA #2 of 3 @19:19:58.43 PSR.select :air @19:19:58.45 PSV.dial PSR @19:19:59.81 Pulling 0.5ml of PSR.wash @19:19:59.82 PSR.select :wash @19:19:59.94 PS.reconfigure :default @19:19:59.99 PS.seek 0.5ml @19:20:02.44 Stroke completed -- waiting for pressure to equalize ... @19:20:02.45 SS.reconfigure :exhale @19:20:02.50 SS.hold @19:20:05.99 delay 5 seconds @19:20:11.02 PSR.select :air @19:20:11.14 PS.seek 2ml @19:20:27.29 PTV.dial :puck @19:20:27.37 PSV.dial PTV @19:20:28.10 PBV.dial :waste1 @19:20:28.53 PS.seek 1.5ml @19:20:29.64 Intake.select :close @19:20:29.80 SSV.dial :exhaust @19:20:30.59 Exhaust.select :open @19:20:30.71 SS.seek 0ml @19:20:34.48 delay 3 seconds @19:20:37.50 PBV.dial between PSV and waste1 @19:20:37.86 PS.seek 0ml @19:20:39.78 Exhaust.select :close @19:20:39.90 SSV.dial CBV @19:20:39.94 CBV.dial SSV @19:20:40.69 Allowing 130s for 25.0ml of remaining 450.0ml @19:20:40.71 Intake.select :open @19:20:42.88 Intake @15.6psia, vacuum from 10.0 to 13.0psi @19:20:42.90 SS.reconfigure :inhaling 0.31ml/s @19:20:42.95 SS.seek 25ml @19:20:53.98 delay 2:00 @19:22:13.19 Stroke completed -- waiting for pressure to equalize ... @19:22:13.20 SS.reconfigure :exhale @19:22:13.25 SS.hold @19:22:50.72 SS.reconfigure :default @19:22:50.77 SS.hold @19:22:50.83 Intake.select :close @19:22:50.99 ShallowSampler::Clogged in WCR -- Filtering too Slowly after 575.0ml @19:22:51.01 SSV.dial :exhaust @19:22:51.77 Exhaust.select :open @19:22:52.30 SS.reconfigure :exhale @19:22:52.43 SS.seek 0ml @19:22:54.00 recoverPSRreagent08 @19:22:54.02 PTV.dial :puck @19:22:54.10 PBV.dial :waste1 @19:22:54.46 delay 30 seconds @19:23:01.52 Exhaust.select :close @19:23:01.63 Sampled 575.0ml @19:23:01.65 Drying puck before fixing for 5:00 @19:23:01.66 CBV.dial :waste2b @19:23:02.43 delay 1:00 @19:23:24.48 PBV.dial between PSV and waste1 @19:23:24.85 PSR.select :air @19:23:24.87 PSV.dial PSR @19:23:25.64 PS.reconfigure :fast @19:23:25.69 PS.seek 2.5ml @19:23:49.50 PSV.dial PTV @19:23:50.27 delay 5 seconds @19:23:55.29 PSV.dial PSR @19:23:56.04 delay 5 seconds @19:24:01.06 PSV.dial PTV @19:24:01.93 delay 5 seconds @19:24:02.45 CSV.dial :air @19:24:02.90 CS.reconfigure :fast @19:24:02.96 CS.seek 10ml @19:24:06.94 PSV.dial :air @19:24:07.71 PS.seek 0ml @19:24:27.48 Pumping wcr puck from tube 3 up to 35psi... @19:24:27.50 CBV.dial SSV @19:24:28.77 CBV.dial between CSV and waste2b @19:24:29.72 CSV.dial between air and CTV @19:24:30.05 CS.seek 7.168ml @19:24:31.10 washCycleDA #3 of 3 @19:24:31.11 PSR.select :air @19:24:31.13 PSV.dial PSR @19:24:32.46 Pulling 0.5ml of PSR.wash @19:24:32.47 PSR.select :wash @19:24:32.59 PS.reconfigure :default @19:24:32.64 PS.seek 0.5ml @19:24:37.42 CTV.dial CSV,avoiding: :air @19:24:37.45 CSV.dial CTV @19:24:38.26 CS.reconfigure :pressurize @19:24:38.32 CS.seek 0ml @19:24:38.54 delay 5 seconds @19:24:43.55 PSR.select :air @19:24:43.67 PS.seek 2ml @19:24:56.78 CBV.dial :waste2b @19:24:57.11 CS.reconfigure :default @19:24:57.16 CS.seek 0ml @19:24:58.32 delay 1:00 @19:24:59.85 PTV.dial :puck @19:25:00.40 PSV.dial PTV @19:25:01.15 PBV.dial :waste1 @19:25:01.58 PS.seek 1.5ml @19:25:07.51 delay 3 seconds @19:25:10.53 PBV.dial between PSV and waste1 @19:25:10.92 PS.seek 0ml @19:25:27.25 delay 2:00 @19:25:58.34 CTV.dial :air,avoiding: :intake @19:25:59.67 Fix #1 of 2 (with RNAlater) for 2:30 @19:25:59.68 CSV.dial CSR @19:26:00.12 Pulling 2ml of CSR.RNAlater @19:26:00.13 CSR.select :RNAlater @19:26:00.25 CS.seek 2ml @19:26:06.41 delay 5 seconds @19:26:11.43 CSR.select :air @19:26:11.56 CS.reconfigure :fast @19:26:11.61 CS.seek 10ml @19:26:31.17 Pumping wcr puck from tube 3 up to 35psi... @19:26:31.19 CBV.dial SSV @19:26:32.45 CBV.dial between CSV and waste2b @19:26:33.41 CSV.dial between air and CTV @19:26:34.01 CS.seek 7.086ml @19:26:41.48 CTV.dial CSV,avoiding: :air @19:26:41.51 CSV.dial CTV @19:26:42.90 CS.reconfigure :pressurize @19:26:42.96 CS.seek 0ml @19:27:01.03 delay 2:30 @19:27:27.28 recoverPSRreagent08 @19:27:27.29 PTV.dial :puck @19:27:27.36 PBV.dial :waste1 @19:27:27.74 delay 30 seconds @19:27:57.76 PBV.dial between PSV and waste1 @19:27:58.12 PSR.select :air @19:27:58.14 PSV.dial PSR @19:27:58.91 PS.reconfigure :fast @19:27:58.96 PS.seek 2.5ml @19:28:22.90 PSV.dial PTV @19:28:23.67 delay 5 seconds @19:28:28.69 PSV.dial PSR @19:28:29.44 delay 5 seconds @19:28:34.46 PSV.dial PTV @19:28:35.20 delay 5 seconds @19:28:40.22 PSV.dial :air @19:28:40.99 PS.seek 0ml @19:29:04.34 Gate.power :camera,:ON @19:29:04.36 Mixing DA Substrates @19:29:04.37 PSR.select :air @19:29:04.39 PSV.dial PSR @19:29:05.78 Pulling 0.25ml of PSR.sub1 @19:29:05.81 PSR.select :sub1 @19:29:06.00 PS.reconfigure :default @19:29:07.43 PS.seek 0.25ml @19:29:10.81 delay 5 seconds @19:29:15.83 Pulling 0.25ml of PSR.sub2 @19:29:15.85 PSR.select :sub2 @19:29:15.97 PS.seek 0.5ml @19:29:19.38 delay 5 seconds @19:29:24.40 PSR.select :air @19:29:24.51 PS.seek 2ml @19:29:31.05 CBV.dial :waste2b @19:29:31.40 CS.reconfigure :default @19:29:31.45 CS.seek 0ml @19:29:32.69 delay 1:00 @19:29:40.72 PTV.dial :puck @19:29:40.80 PSV.dial PTV @19:29:41.54 PBV.dial :waste1 @19:29:41.98 PS.seek 1.5ml @19:29:47.90 delay 3 seconds @19:29:50.92 PBV.dial between PSV and waste1 @19:29:51.30 PS.seek 0ml @19:30:07.47 delay 10 seconds @19:30:17.49 recoverPSRreagent08 @19:30:17.50 PTV.dial :puck @19:30:17.58 PBV.dial :waste1 @19:30:17.95 delay 30 seconds @19:30:32.72 CTV.dial :air,avoiding: :intake @19:30:34.07 Fix #2 of 2 (with RNAlater) for 2:30 @19:30:34.09 CSV.dial CSR @19:30:34.55 Pulling 2ml of CSR.RNAlater @19:30:34.56 CSR.select :RNAlater @19:30:34.68 CS.seek 2ml @19:30:40.60 delay 5 seconds @19:30:45.62 CSR.select :air @19:30:45.74 CS.reconfigure :fast @19:30:45.79 CS.seek 10ml @19:30:47.97 PBV.dial between PSV and waste1 @19:30:48.34 PSR.select :air @19:30:48.36 PSV.dial PSR @19:30:49.13 PS.reconfigure :fast @19:30:49.18 PS.seek 2.5ml @19:31:05.88 Pumping wcr puck from tube 3 up to 35psi... @19:31:05.90 CBV.dial SSV @19:31:07.17 CBV.dial between CSV and waste2b @19:31:08.15 CSV.dial between air and CTV @19:31:08.76 CS.seek 7.086ml @19:31:13.37 PSV.dial PTV @19:31:14.14 delay 5 seconds @19:31:16.60 CTV.dial CSV,avoiding: :air @19:31:16.63 CSV.dial CTV @19:31:18.04 CS.reconfigure :pressurize @19:31:18.59 CS.seek 0ml @19:31:19.15 PSV.dial PSR @19:31:19.92 delay 5 seconds @19:31:24.94 PSV.dial PTV @19:31:25.72 delay 5 seconds @19:31:30.74 PSV.dial :air @19:31:31.52 PS.seek 0ml @19:31:32.43 delay 2:30 @19:31:55.69 Additional air clear @19:31:55.70 PSR.select :air @19:31:55.71 PSV.dial PSR @19:31:57.06 Pulling 2ml of PSR.air @19:31:57.07 PSR.select :air @19:31:57.09 PS.reconfigure :default @19:31:57.14 PS.seek 2ml @19:32:18.43 delay 5 seconds @19:32:23.45 PTV.dial :puck @19:32:23.54 PSV.dial PTV @19:32:24.31 PBV.dial :waste1 @19:32:24.75 PS.seek 1.5ml @19:32:30.62 delay 3 seconds @19:32:33.64 PBV.dial between PSV and waste1 @19:32:34.00 delay 1 second @19:32:35.02 PSV.dial :waste1 @19:32:36.08 PS.seek 0ml @19:32:52.23 Processing Puck Heater at 28.1C before imaging @19:32:52.24 PH.coast @19:32:52.36 Imaging da puck in Processing Clamp while storing it to tube 2 @19:32:52.54 SC.reconfigure :tight @19:32:52.59 SC.seek 2 @19:32:56.11 SC.configure :limp @19:32:56.33 Elbow.reconfigure :tight @19:32:56.38 Elbow.seek 2 @19:32:58.14 Forearm.reconfigure :tight @19:32:58.19 Forearm.seek 2 + 450 counts @19:32:59.20 SE.reconfigure :pick @19:32:59.25 SE.seek :beamBreak @19:33:01.38 SE.configure :default @19:33:01.54 Elbow.reconfigure :limp @19:33:01.60 Forearm.reconfigure :loose @19:33:01.64 Forearm.seek 2 @19:33:01.96 Elbow.hold @19:33:02.03 Elbow.reconfigure :tight @19:33:02.13 SE.seek 16.11 Pucks below Top Plate @19:33:03.18 Forearm.force -20 @19:33:03.23 Forearm.seek 2 + 304 counts @19:33:03.53 Forearm.reconfigure :tight @19:33:03.58 Forearm.seek 2 + 450 counts @19:33:03.78 SE.seek 17.11 Pucks below Top Plate @19:33:04.33 Forearm.reconfigure :loose @19:33:04.38 Forearm.seek :retract @19:33:05.29 Elbow.seek PC @19:33:07.78 Elbow.reconfigure :loose @19:33:07.89 Forearm.seek PC @19:33:08.82 Hand.close @19:33:09.42 PC.seek :open @19:33:14.21 Forearm.seek :retract @19:33:15.18 Elbow.reconfigure :tight @19:33:15.23 Elbow.seek 2 @19:33:17.84 Forearm.reconfigure :tight @19:33:17.89 Forearm.seek 2 + 450 counts @19:33:18.93 Hand.open @19:33:19.60 SE.seek 16.76 Pucks below Top Plate @19:33:19.90 SE.reconfigure :seal @19:33:19.95 SE.seek 15.8 Pucks below Top Plate @19:33:20.49 Lights, camera, ACTION! @19:33:20.53 PuckCamera: snap -bin=2,2 -auto /var/log/esp/da23oct2719h625ml.tif @19:33:40.00 Waiting >20 seconds for da to relinquish FlushPuck @19:33:44.83 SX HX9/16 (USB:13): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 21.023 seconds /var/log/esp/da23oct2719h625ml.tif: TIFF Upload Complete @19:33:44.86 PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da23oct2719h625ml15s.tif @19:34:00.51 SX HX9/16 (USB:13): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15 seconds /var/log/esp/midres/da23oct2719h625ml15s.tif: TIFF Upload Complete @19:34:00.55 PuckCamera: snap -bin=2,2 20.0 /var/log/esp/midres/da23oct2719h625ml20s.tif @19:34:02.45 CBV.dial :waste2b @19:34:02.82 CS.reconfigure :default @19:34:02.88 CS.seek 0ml @19:34:08.63 delay 1:00 @19:34:21.20 SX HX9/16 (USB:13): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 20 seconds /var/log/esp/midres/da23oct2719h625ml20s.tif: TIFF Upload Complete @19:34:21.22 Gate.power :camera,:OFF @19:34:21.23 DAfans.email "21.023 sec auto-exposure",Subject: "da23oct2719h625ml images!" @19:34:21.67 Elbow.seek 2 @19:34:21.91 Forearm.seek 2 + 450 counts @19:34:22.60 SE.reconfigure :gently @19:34:22.66 SE.seek 16.51 Pucks below Top Plate @19:34:23.31 Elbow.reconfigure :limp @19:34:23.36 Forearm.reconfigure :loose @19:34:23.41 Forearm.seek 2 @19:34:23.73 Elbow.hold @19:34:23.80 Elbow.reconfigure :tight @19:34:23.86 SE.reconfigure :default @19:34:23.96 SE.seek 16.7 Pucks below Top Plate @19:34:24.33 Forearm.force -20 @19:34:24.38 Forearm.seek 2 + 304 counts @19:34:25.01 Forearm.reconfigure :tight @19:34:25.13 Forearm.seek 2 + 450 counts @19:34:25.42 SE.seek :down @19:34:26.69 Forearm.reconfigure :loose @19:34:26.81 Forearm.seek :retract @19:34:27.02 SE.stop @19:34:27.92 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @19:34:28.32 Elbow.seek FlushPuck::Garage @19:34:30.29 Elbow.reconfigure :loose @19:34:30.52 Forearm.seek :garage @19:34:31.62 Hand.close @19:34:32.57 Forearm.seek :retract @19:34:33.62 Elbow.reconfigure :tight @19:34:33.72 Elbow.seek PC @19:34:35.11 Elbow.reconfigure :loose @19:34:35.33 Forearm.reconfigure :tight @19:34:35.44 Forearm.seek PC @19:34:38.13 PC.seek :closed @19:34:42.91 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.64 @19:34:43.09 Hand.open @19:34:43.84 Forearm.reconfigure :loose @19:34:43.95 Forearm.seek :retract @19:34:44.95 PSR.select :air @19:34:44.99 PSV.dial PSR @19:34:45.52 Pulling 0.5ml of PSR.flush @19:34:45.54 PSR.select :flush @19:34:45.71 PS.seek 0.5ml @19:34:51.74 delay 5 seconds @19:34:56.24 upsync @19:34:56.77 PSR.select :air @19:34:56.89 PS.seek 2.5ml @19:35:08.66 CTV.dial :air,avoiding: :intake @19:35:10.01 Storing wcr puck in Collection Clamp to tube 2 @19:35:10.45 Elbow.reconfigure :tight @19:35:10.50 Elbow.seek 2 @19:35:13.10 Forearm.reconfigure :tight @19:35:13.15 Forearm.seek 2 + 450 counts @19:35:14.21 SE.reconfigure :pick @19:35:14.27 SE.seek :beamBreak @19:35:16.18 SE.configure :default @19:35:16.34 Elbow.reconfigure :limp @19:35:16.40 Forearm.reconfigure :loose @19:35:16.44 Forearm.seek 2 @19:35:17.09 Elbow.hold @19:35:17.20 Elbow.reconfigure :tight @19:35:17.31 SE.seek 17.12 Pucks below Top Plate @19:35:17.79 Forearm.force -20 @19:35:17.84 Forearm.seek 2 + 314 counts @19:35:18.16 delay 1 second @19:35:18.27 Forearm.reconfigure :tight @19:35:18.32 Forearm.seek 2 + 450 counts @19:35:18.58 SE.seek 18.12 Pucks below Top Plate @19:35:19.14 Forearm.reconfigure :loose @19:35:19.20 Forearm.seek :retract @19:35:19.25 PRV.dial :waste2,avoiding: :ab2 @19:35:20.18 Elbow.seek CC @19:35:21.12 PTV.dial :PRVmixing @19:35:21.82 Elbow.reconfigure :loose @19:35:21.92 Forearm.seek CC @19:35:22.43 PSV.dial :mixloop @19:35:22.95 Hand.close @19:35:23.46 PS.seek :empty @19:35:23.54 CC.seek :open @19:35:28.30 Forearm.seek :retract @19:35:29.29 Elbow.reconfigure :tight @19:35:29.34 Elbow.seek 2 @19:35:31.17 Elbow.reconfigure :limp @19:35:31.23 Forearm.seek 2 @19:35:32.27 Elbow.hold @19:35:32.35 Elbow.reconfigure :tight @19:35:32.84 Hand.open @19:35:33.55 Forearm.force -20 @19:35:33.60 Forearm.seek 2 + 313 counts @19:35:33.91 Forearm.reconfigure :tight @19:35:33.97 Forearm.seek 2 + 450 counts @19:35:34.25 SE.seek :down @19:35:35.39 Forearm.reconfigure :loose @19:35:35.44 Forearm.seek :retract @19:35:35.72 SE.stop @19:35:36.37
Starting finalPurge @19:35:49.99 PSR.select :air @19:35:50.01 PSV.dial PSR @19:35:51.05 Pulling 0.5ml of PSR.flush @19:35:51.07 PSR.select :flush @19:35:51.19 PS.seek 0.5ml @19:35:55.77
Waiting >20 seconds for psFlush to relinquish FlushPuck @19:35:57.05 delay 5 seconds @19:36:02.08 PSR.select :air @19:36:02.19 PS.seek 2.5ml @19:36:23.46 delay 1 second @19:36:24.48 PBV.dial :waste1 @19:36:24.83 PTV.dial :mixing @19:36:25.58 PSV.dial :mixloop @19:36:26.63 PS.seek :empty @19:36:53.02 PSV.dial PSR @19:36:54.07 PSR.select :flush @19:36:54.19 PS.seek 1ml @19:37:05.30 PSR.select :air @19:37:05.42 PS.seek 2.5ml @19:37:21.61 PSV.dial PTV @19:37:22.38 PTV.dial :puck @19:37:23.11 PBV.dial :waste1 @19:37:23.20 PS.seek 0ml @19:37:49.52 PSV.dial PSR @19:37:50.27 PSR.select :flush @19:37:50.39 PS.seek 0.5ml @19:37:56.36 PSR.select :air @19:37:56.48 PS.seek 2.5ml @19:38:17.71 PSV.dial PTV @19:38:18.46 PBV.dial PSV @19:38:19.07 PS.seek 0ml @19:38:45.42 PSV.dial PBV @19:38:45.90 PS.seek 2.5ml @19:39:12.30 PSV.dial :waste1 @19:39:13.04 PS.seek 0ml @19:39:39.49 PSV.dial PSR @19:39:39.99 PSR.select :air @19:39:40.02 PS.seek 2.5ml @19:40:06.33 PSV.dial PTV @19:40:07.06 PBV.dial PSV @19:40:07.15 PS.seek 0ml @19:40:33.52 PSV.dial PBV @19:40:33.99 PS.seek 2.5ml @19:41:00.30 PSV.dial :waste1 @19:41:01.04 PS.seek 0ml @19:41:27.42 PSV.dial PSR @19:41:27.89 PSR.select :flush @19:41:28.01 PS.seek 1ml @19:41:39.14 PSR.select :air @19:41:39.26 PS.seek 2.5ml @19:41:55.42 PSV.dial PTV @19:41:56.16 PTV.dial :puck @19:41:56.23 PBV.dial :waste1 @19:41:56.80 PS.seek 0ml @19:42:23.08 PSV.dial PSR @19:42:23.83 PSR.select :flush @19:42:23.95 PS.seek 0.5ml @19:42:29.89 PSR.select :air @19:42:30.01 PS.seek 2.5ml @19:42:51.20 PSV.dial PTV @19:42:51.95 PBV.dial PSV @19:42:52.50 PS.seek 0ml @19:43:18.86 PSV.dial PBV @19:43:19.33 PS.seek 2.5ml @19:43:45.64 PSV.dial :waste1 @19:43:46.38 PS.seek 0ml @19:44:12.92 PSV.dial PSR @19:44:13.39 PSR.select :air @19:44:13.41 PS.seek 2.5ml @19:44:39.79 PSV.dial PTV @19:44:40.53 PBV.dial PSV @19:44:40.61 PS.seek 0ml @19:45:06.98 PSV.dial PBV @19:45:07.52 PS.seek 2.5ml @19:45:33.82 PSV.dial :waste1 @19:45:34.56 PS.seek 0ml @19:46:00.95
Loading FlushPuck into Collection Clamp from Processing Clamp @19:46:01.15 Elbow.seek PC @19:46:03.63 Elbow.reconfigure :loose @19:46:03.74 Forearm.seek PC @19:46:04.67 Hand.close @19:46:05.28 PC.seek :open @19:46:10.01 Forearm.seek :retract @19:46:10.97 Elbow.reconfigure :tight @19:46:11.02 Elbow.seek CC @19:46:14.65 Elbow.reconfigure :loose @19:46:14.75 Forearm.reconfigure :tight @19:46:14.80 Forearm.seek CC @19:46:16.02 CC.seek :closed @19:46:20.55 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.29 @19:46:20.61 Hand.open @19:46:21.34 Forearm.reconfigure :loose @19:46:21.39 Forearm.seek :retract @19:46:22.38 Flushing the Collection Valve::Manifold @19:46:22.50 CSV.dial CSR @19:46:23.05 Pulling 4.999ml of CSR.flush @19:46:23.06 CSR.select :flush @19:46:23.18 CS.seek 5ml @19:46:36.74 delay 5 seconds @19:46:41.76 CSR.select :air @19:46:41.94 CS.reconfigure :fast @19:46:41.99 CS.seek 7.501ml @19:46:49.24 CSV.dial :waste @19:46:50.51 CS.reconfigure :default @19:46:50.56 CS.seek 0ml @19:47:10.58 Flushing lower manifold's waste1 port @19:47:10.71 CSV.dial :flush @19:47:11.16 CS.reconfigure :fast @19:47:11.21 CS.seek 5ml @19:47:23.89 delay 5 seconds @19:47:28.91 CSV.dial :air @19:47:29.43 CS.seek 7ml @19:47:34.93 CSV.dial CTV @19:47:34.95
CTV.dial CSV @19:47:36.31 CBV.dial :waste1 @19:47:37.09 CS.seek 0ml @19:47:53.64 Flushing lower manifold's waste2 port @19:47:53.77 CSV.dial :flush @19:47:54.51 CS.seek 5ml @19:48:07.17 delay 5 seconds @19:48:12.19 CSV.dial :air @19:48:12.72 CS.seek 7.001ml @19:48:18.21 CSV.dial CTV @19:48:18.23
CTV.dial CSV @19:48:18.69 CBV.dial :waste2 @19:48:19.74 CS.seek 0ml @19:48:36.39 Flushing lower manifold's waste2b port @19:48:36.52 CSV.dial :flush @19:48:37.24 CS.seek 5ml @19:48:49.86 delay 5 seconds @19:48:54.88 CSV.dial :air @19:48:55.41 CS.seek 7ml @19:49:00.99 CSV.dial CTV @19:49:01.01
CTV.dial CSV @19:49:01.47 CBV.dial :waste2b @19:49:02.51 CS.seek 0ml @19:49:19.02 Disinfecting external Intake with kill @19:49:19.14 CSV.dial CSR @19:49:19.58 Pulling 8ml of CSR.kill @19:49:19.59 CSR.select :kill @19:49:19.71 CS.seek 8ml @19:49:39.49 delay 5 seconds @19:49:44.50 CSR.select :air @19:49:44.63 CS.seek 10ml @19:49:50.17 CSV.dial CBV @19:49:50.20
CBV.dial CSV @19:49:51.70 CTV.dial :intake @19:49:52.50 Intake.select :open @19:49:52.61 CS.seek 2ml @19:50:11.45 Intake.select :close @19:50:11.55 CTV.dial :air @19:50:12.30 CS.reconfigure :default @19:50:12.35 CS.seek 0ml @19:50:18.45 CSV.dial CSR @19:50:19.47 Pulling 5ml of CSR.flush @19:50:19.48 CSR.select :flush @19:50:19.60 CS.seek 5ml @19:50:33.26 delay 5 seconds @19:50:38.28 CSR.select :air @19:50:38.41 CS.seek 10ml @19:50:51.95 CSV.dial CBV @19:50:52.96 CS.seek 0ml @19:51:19.36 Garaging FlushPuck in Collection Clamp @19:51:19.43 Elbow.reconfigure :tight @19:51:19.48 Elbow.seek CC @19:51:19.72 Elbow.reconfigure :loose @19:51:19.82 Forearm.seek CC @19:51:20.90 Hand.close @19:51:21.53 CC.seek :open @19:51:26.16 Forearm.seek :retract @19:51:27.15 Elbow.reconfigure :tight @19:51:27.21 Elbow.seek :garage @19:51:30.13 Elbow.reconfigure :loose @19:51:30.24 Forearm.reconfigure :tight @19:51:30.29 Forearm.seek :garage @19:51:31.35 Hand.open @19:51:32.09 Forearm.reconfigure :loose @19:51:32.14 Forearm.seek :retract @19:51:33.12 Completed [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] begun 17:27:15 @19:51:33.17 Asleep after completing DA_WCR_102723 assays @19:51:33.20 GoodNews.email "{1 => 33,\n 2 => 19,\n 3 => 14,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@19:46:41, 25% flow, 21.8C, 22% humidity, 14.8psia, 13.695V, 0.453A, 232.618Ah, 6.21W\nSampled 575.0ml",Subject: "Asleep after completing DA_WCR_102723 assays" @19:51:37.05 upsync + ESPwhich DA_WCR_103123.rb + fn=DA_WCR_103123.rb + IFS=: + set /home/esp/esp2/mission . + fullPath=/home/esp/esp2/mission/DA_WCR_103123.rb + [ -f /home/esp/esp2/mission/DA_WCR_103123.rb ] + realpath /home/esp/esp2/mission/DA_WCR_103123.rb + return 0 + scriptPath=/home/esp/esp2local/mission/DA_WCR_103123.rb + exec ruby /home/esp/esp2/lib/esp.rb /home/esp/esp2local/mission/DA_WCR_103123.rb @13:50:46.42UTC31-Oct-23
ESP2 v3.42 I2C Gateway v3.20 for core bus @13:50:49.19 Configuration for short puck jake 1/11/21 by brent@mbari.org /home/esp/esp2/type/shallow/jake/configure.rb @13:50:52.97 Polling Can every 10:00 @13:50:52.99 Cleanly exited at 13:48:15UTC31-Oct-23 @13:50:53.04 Loading /home/esp/esp2local/mission/DA_WCR_103123.rb mission @13:50:53.10 GoodNews.email "Starting DA_WCR_103123 w/pucks in tube 3\n{}\nCTD data unavailable\nCan@13:50:52, 27% flow, 17.9C, 23% humidity, 14.6psia, 13.749V, 0.272A, 253.333Ah, 3.74W",Subject: "DA_WCR_103123 begun" @13:50:53.14
Preparing to sleep until + 00:00:05 @13:50:53.17 Asleep til + 00:00:05 @13:50:53.19 Continuing at 13:50 @13:50:53.47 Gate.power :core,:OFF @13:50:53.49 Gate.power :raw,:OFF @13:50:53.50 Shutdown @13:50:56.17 GoodNews.email "Then, in tube 3: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_103123.rb:14\n{1 => 33,\n 2 => 19,\n 3 => 14,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@13:50:53, 27% flow, 17.9C, 23% humidity, 14.6psia, 13.749V, 0.272A, 253.333Ah, 3.74W",Subject: "Asleep until 13:50" @13:50:57.56 upsync @13:50:58.07
Powering core bus @13:51:00.33 upsync @13:51:03.97
Gate.power :raw,:ON @13:51:04.95 CS.configure :default @13:51:05.00 PS.configure :default @13:51:05.05 SS.configure :default @13:51:05.10 CC.configure :default @13:51:05.15 PC.configure :default @13:51:05.22 Forearm.configure :loose @13:51:05.26 Elbow.configure :loose @13:51:05.33 SC.configure :loose @13:51:05.38 SE.configure :default @13:51:05.43 CH.configure :default @13:51:05.46 PH.configure :default @13:51:05.50 All dwarf controllers running firmware version 3.85 @13:51:05.61 Valve.reset @13:51:05.87 Intake.select :close @13:51:05.91 Exhaust.select :close @13:51:05.92 CTV.dial :air @13:51:06.04 PSV.dial :air @13:51:06.09 CSV.dial :waste1 @13:51:06.20
Forearm.home.seek :retract @13:51:06.55 SSV.dial :spit,avoiding: :exhaust @13:51:06.96 Hand.close @13:51:07.00
Elbow.reconfigure :loose @13:51:07.09 Elbow.jog 1500 @13:51:07.29 PS.home.seek 0ml @13:51:07.42 CS.reconfigure :fast @13:51:07.46 SS.home.seek 0ml @13:51:07.56 CS.home.seek 0ml @13:51:07.63
Elbow.reconfigure :loose @13:51:07.68 Elbow.home.seek :clear @13:51:10.96 SE.home.seek :down @13:51:11.60 SC.reconfigure :tight @13:51:11.65 SC.home.seek :initial @13:51:15.63 PS.stop @13:51:39.97
SC.configure :limp @13:51:40.02 Forearm.seek :retract @13:51:40.09 Elbow.seek :hammer @13:51:41.23 Forearm.reconfigure :tight @13:51:41.28 Forearm.seek retracted @13:51:41.54 CC.home.seek :open @13:51:41.56 PC.home.seek :open @13:51:45.25
Phase [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] @13:51:45.27 Notice.email "At 13:51:45 began in tube 3: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_103123.rb:14\n{1 => 33,\n 2 => 19,\n 3 => 14,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@13:51:45, 27% flow, 17.9C, 23% humidity, 14.6psia, 13.720V, 0.284A, 253.339Ah, 3.90W",Subject: "Started DA_WCR_103123 phase initialPurge; da [1000, 1000] #da and wsr are run simultanelously" @13:51:45.29
Starting initialPurge @13:51:45.32 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @13:51:45.38 Hand.open @13:51:46.14 Elbow.reconfigure :tight @13:51:46.21 Elbow.seek FlushPuck::Garage @13:51:48.17 Elbow.reconfigure :loose @13:51:48.28 Forearm.reconfigure :loose @13:51:48.34 Forearm.seek :garage @13:51:49.26 upsync @13:51:49.31
Hand.close @13:51:50.01 Forearm.seek :retract @13:51:50.96 Elbow.reconfigure :tight @13:51:51.00 Elbow.seek CC @13:51:53.95 Elbow.reconfigure :loose @13:51:54.06 Forearm.reconfigure :tight @13:51:54.11 Forearm.seek CC @13:51:55.32 CC.seek :closed @13:51:59.94 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.37 @13:52:00.00 Hand.open @13:52:00.69 Forearm.reconfigure :loose @13:52:00.74 Forearm.seek :retract @13:52:01.71 Flushing the Collection Valve::Manifold @13:52:01.84 CSV.dial CSR @13:52:03.24 Pulling 5ml of CSR.flush @13:52:03.25 CSR.select :flush @13:52:03.37 CS.reconfigure :default @13:52:03.82 CS.seek 5ml @13:52:17.64 delay 5 seconds @13:52:22.66 CSR.select :air @13:52:22.83 CS.reconfigure :fast @13:52:22.88 CS.seek 7.5ml @13:52:29.61 CSV.dial :waste @13:52:30.90 CS.reconfigure :default @13:52:30.95 CS.seek 0ml @13:52:50.96 Purging external Intake @13:52:51.08 CSV.dial :flush @13:52:51.54 CS.reconfigure :fast @13:52:51.59 CS.seek 10ml @13:53:16.02 delay 5 seconds @13:53:21.04 CSV.dial CBV @13:53:21.08
CBV.dial CSV @13:53:21.83 CTV.dial :intake @13:53:22.63 Intake.select :open @13:53:22.74 CS.seek 0ml @13:53:46.27 Intake.select :close @13:53:46.37 CTV.dial :air @13:53:47.16 Flushing upper manifold @13:53:47.28 CSV.dial :flush @13:53:48.02 CS.seek 5ml @13:54:00.83 delay 5 seconds @13:54:05.85 CSV.dial :air @13:54:06.47 CS.seek 7ml @13:54:11.99 CSV.dial CBV @13:54:12.02
CBV.dial CSV @13:54:13.03 CTV.dial :air @13:54:13.12 CS.seek 0ml @13:54:29.71 delay 7 seconds @13:54:36.73 CS.seek 3ml @13:54:44.73 CSV.dial :waste @13:54:45.20 CS.seek 0ml @13:54:52.94 CSV.dial :flush @13:54:53.38 CS.seek 3ml @13:55:01.55 delay 5 seconds @13:55:06.57 CSV.dial :air @13:55:07.19 CS.seek 5ml @13:55:12.76 CSV.dial :air @13:55:12.85 CS.seek 0ml @13:55:25.05 delay 7 seconds @13:55:32.07 CS.seek 3ml @13:55:40.11 CSV.dial :waste @13:55:40.83 CS.seek 0ml @13:55:48.52 Garaging FlushPuck in Collection Clamp @13:55:48.59 Elbow.reconfigure :tight @13:55:48.63 Elbow.seek CC @13:55:48.95 Elbow.reconfigure :loose @13:55:49.06 Forearm.seek CC @13:55:50.08 Hand.close @13:55:50.76 CC.seek :open @13:55:55.82 Forearm.seek :retract @13:55:56.79 Elbow.reconfigure :tight @13:55:56.84 Elbow.seek :garage @13:55:59.79 Elbow.reconfigure :loose @13:55:59.90 Forearm.reconfigure :tight @13:55:59.95 Forearm.seek :garage @13:56:00.93 Hand.open @13:56:01.57 Forearm.reconfigure :loose @13:56:01.61 Forearm.seek :retract @13:56:02.57 da sampling at most 1000ml, wcr at most 1000ml @13:56:02.61 Loading sh1 puck from tube 3 into Collection Clamp @13:56:02.62 DAfans.email "Started da in tube 3 at 13:56:02UTC31-Oct-23\n{1 => 33,\n 2 => 19,\n 3 => 14,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@13:51:45, 27% flow, 17.9C, 23% humidity, 14.6psia, 13.720V, 0.284A, 253.339Ah, 3.90W",Subject: "da sampling at most 1000ml, wcr at most 1000ml" @13:56:02.90
SC.reconfigure :tight @13:56:02.95 SC.seek 3 @13:56:06.46 upsync @13:56:06.53
SC.configure :limp @13:56:06.76 Elbow.reconfigure :tight @13:56:06.81 Elbow.seek 3 @13:56:08.80 Forearm.reconfigure :tight @13:56:08.85 Forearm.seek 3 + 450 counts @13:56:09.90 SE.reconfigure :pick @13:56:09.96 SE.seek :beamBreak @13:56:12.29 SE.configure :default @13:56:12.45 Elbow.seek 3 @13:56:12.64 Forearm.seek 3 + 300 counts @13:56:12.82 Forearm.reconfigure :loose @13:56:12.86 Hand.close @13:56:13.52 SE.seek :down @13:56:14.09
Forearm.seek :retract @13:56:15.10 Elbow.seek CC @13:56:15.45 SE.stop @13:56:16.72
Elbow.reconfigure :loose @13:56:16.82 Forearm.reconfigure :tight @13:56:16.87 Forearm.seek CC @13:56:18.07 CC.seek :closed @13:56:22.58 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.24 @13:56:22.59 Hand.open @13:56:23.32 Forearm.reconfigure :loose @13:56:23.36 Forearm.seek :retract @13:56:24.33 collectSample 1000ml w/bubble point of 24psi @13:56:24.35 Priming 7ml @13:56:24.36 Intake.select :close @13:56:24.37 Exhaust.select :close @13:56:24.38 SS.hold @13:56:24.59 Calibrating pressure and temperature sensors @13:56:24.62 CTV.dial :air @13:56:24.70 SSV.dial :spit,avoiding: :exhaust @13:56:24.78 SS.reconfigure :fast @13:56:24.83 SS.seek 5ml @13:56:27.67 SSV.dial CBV @13:56:27.70
CBV.dial SSV @13:56:29.03 delay 3.8 seconds @13:56:32.95 Sensor limits: Intake max 108.3psia; Outlet max 100.9psia @13:56:32.96 SSV.dial :spit @13:56:34.30 SS.seek 0ml @13:56:37.04 SSV.dial CBV @13:56:38.36 CTV.dial :intake @13:56:39.16 SSV.dial CBV @13:56:39.18
CBV.dial SSV @13:56:39.39 Allowing 40s for 7.0ml of remaining 7.0ml @13:56:39.40 Intake.select :open @13:56:41.56 Intake @14.7psia, vacuum from 9.2 to 12.2psi @13:56:41.58 SS.reconfigure :inhaling 2.20ml/s @13:56:41.63 SS.seek 7ml @13:56:48.57 Stroke completed -- waiting for pressure to equalize ... @13:56:48.58 SS.reconfigure :exhale @13:56:48.63 SS.hold @13:56:54.90 Intake.select :close @13:56:55.06 SSV.dial :exhaust @13:56:55.82 Exhaust.select :open @13:56:55.94 SS.seek 0ml @13:56:59.82 Exhaust.select :close @13:56:59.94 Sampling 1000.0ml @13:56:59.95 Intake.select :close @13:56:59.96 Exhaust.select :close @13:56:59.97 SS.reconfigure :default @13:57:00.01 SS.hold @13:57:00.22 CTV.dial :intake @13:57:00.41 SSV.dial CBV @13:57:00.44
CBV.dial SSV @13:57:01.21 Allowing 130s for 25.0ml of remaining 1000.0ml @13:57:01.23 Intake.select :open @13:57:03.39 Intake @15.3psia, vacuum from 9.8 to 12.8psi @13:57:03.41 SS.reconfigure :inhaling 2.20ml/s @13:57:03.45 SS.seek 25ml @13:57:12.42 Vacuum too high -- waiting up to 40 seconds @13:57:12.44 SS.reconfigure :exhale @13:57:12.49 SS.hold @13:57:17.74 Intake @16.3psia, vacuum from 10.0 to 13.0psi @13:57:17.76 SS.reconfigure :inhaling 1.76ml/s @13:57:17.80 SS.seek 25ml @13:57:22.52 Vacuum too high -- waiting up to 40 seconds @13:57:22.53 SS.reconfigure :exhale @13:57:22.58 SS.hold @13:57:26.01 Intake @16.8psia, vacuum from 10.0 to 13.0psi @13:57:26.03 SS.reconfigure :inhaling 1.41ml/s @13:57:26.08 SS.seek 25ml @13:57:30.10 Stroke completed -- waiting for pressure to equalize ... @13:57:30.11 SS.reconfigure :exhale @13:57:30.16 SS.hold @13:57:42.81 Intake.select :close @13:57:42.96 SSV.dial :exhaust @13:57:43.73 Exhaust.select :open @13:57:43.84 SS.seek 0ml @13:57:52.59 Exhaust.select :close @13:57:52.70 SSV.dial CBV @13:57:52.73
CBV.dial SSV @13:57:53.52 Allowing 130s for 25.0ml of remaining 975.0ml @13:57:53.53 Intake.select :open @13:57:55.69 Intake @15.8psia, vacuum from 10.0 to 13.0psi @13:57:55.71 SS.reconfigure :inhaling 1.41ml/s @13:57:55.76 SS.seek 25ml @13:58:08.74 Vacuum too high -- waiting up to 40 seconds @13:58:08.75 SS.reconfigure :exhale @13:58:08.80 SS.hold @13:58:13.12 Intake @16.0psia, vacuum from 10.0 to 13.0psi @13:58:13.13 SS.reconfigure :inhaling 1.13ml/s @13:58:13.18 SS.seek 25ml @13:58:23.02 Stroke completed -- waiting for pressure to equalize ... @13:58:23.03 SS.reconfigure :exhale @13:58:23.08 SS.hold @13:58:39.72 Intake.select :close @13:58:39.88 SSV.dial :exhaust @13:58:40.67 Exhaust.select :open @13:58:40.78 SS.seek 0ml @13:58:49.85 Exhaust.select :close @13:58:49.96 SSV.dial CBV @13:58:49.98
CBV.dial SSV @13:58:50.81 Allowing 130s for 25.0ml of remaining 950.0ml @13:58:50.82 Intake.select :open @13:58:52.99 Intake @15.9psia, vacuum from 10.0 to 13.0psi @13:58:53.00 SS.reconfigure :inhaling 1.13ml/s @13:58:53.05 SS.seek 25ml @13:59:17.21 Stroke completed -- waiting for pressure to equalize ... @13:59:17.22 SS.reconfigure :exhale @13:59:17.27 SS.hold @13:59:33.56 Intake.select :close @13:59:33.71 SSV.dial :exhaust @13:59:34.49 Exhaust.select :open @13:59:34.60 SS.seek 0ml @13:59:43.81 Exhaust.select :close @13:59:43.92 SSV.dial CBV @13:59:43.94
CBV.dial SSV @13:59:44.74 Allowing 130s for 25.0ml of remaining 925.0ml @13:59:44.75 Intake.select :open @13:59:46.92 Intake @15.8psia, vacuum from 10.0 to 13.0psi @13:59:46.94 SS.reconfigure :inhaling 1.13ml/s @13:59:46.99 SS.seek 25ml @14:00:11.61 Stroke completed -- waiting for pressure to equalize ... @14:00:11.62 SS.reconfigure :exhale @14:00:11.67 SS.hold @14:00:27.98 Intake.select :close @14:00:28.14 SSV.dial :exhaust @14:00:28.91 Exhaust.select :open @14:00:29.03 SS.seek 0ml @14:00:37.97 Exhaust.select :close @14:00:38.08 SSV.dial CBV @14:00:38.11
CBV.dial SSV @14:00:38.90 Allowing 130s for 25.0ml of remaining 900.0ml @14:00:38.91 Intake.select :open @14:00:41.07 Intake @15.8psia, vacuum from 10.0 to 13.0psi @14:00:41.09 SS.reconfigure :inhaling 1.13ml/s @14:00:41.14 SS.seek 25ml @14:01:05.46 Stroke completed -- waiting for pressure to equalize ... @14:01:05.47 SS.reconfigure :exhale @14:01:05.52 SS.hold @14:01:20.16 Intake.select :close @14:01:20.31 SSV.dial :exhaust @14:01:21.08 Exhaust.select :open @14:01:21.20 SS.seek 0ml @14:01:30.16 Exhaust.select :close @14:01:30.27 SSV.dial CBV @14:01:30.29
CBV.dial SSV @14:01:31.08 Allowing 130s for 25.0ml of remaining 875.0ml @14:01:31.09 Intake.select :open @14:01:33.26 Intake @15.8psia, vacuum from 10.0 to 13.0psi @14:01:33.28 SS.reconfigure :inhaling 1.13ml/s @14:01:33.33 SS.seek 25ml @14:01:56.82 Vacuum too high -- waiting up to 40 seconds @14:01:56.84 SS.reconfigure :exhale @14:01:56.89 SS.hold @14:02:04.54 Intake @16.3psia, vacuum from 10.0 to 13.0psi @14:02:04.56 SS.reconfigure :inhaling 0.91ml/s @14:02:04.61 SS.seek 25ml @14:02:06.10 Stroke completed -- waiting for pressure to equalize ... @14:02:06.11 SS.reconfigure :exhale @14:02:06.15 SS.hold @14:02:13.61 Intake.select :close @14:02:13.76 SSV.dial :exhaust @14:02:14.52 Exhaust.select :open @14:02:14.63 SS.seek 0ml @14:02:23.57 Exhaust.select :close @14:02:23.68 SSV.dial CBV @14:02:23.71
CBV.dial SSV @14:02:24.47 Allowing 130s for 25.0ml of remaining 850.0ml @14:02:24.48 Intake.select :open @14:02:26.65 Intake @15.7psia, vacuum from 10.0 to 13.0psi @14:02:26.67 SS.reconfigure :inhaling 0.91ml/s @14:02:26.71 SS.seek 25ml @14:02:56.03 Stroke completed -- waiting for pressure to equalize ... @14:02:56.04 SS.reconfigure :exhale @14:02:56.09 SS.hold @14:03:08.91 Intake.select :close @14:03:09.06 SSV.dial :exhaust @14:03:09.82 Exhaust.select :open @14:03:09.93 SS.seek 0ml @14:03:18.66 Exhaust.select :close @14:03:18.77 SSV.dial CBV @14:03:18.79
CBV.dial SSV @14:03:19.58 Allowing 130s for 25.0ml of remaining 825.0ml @14:03:19.60 Intake.select :open @14:03:21.76 Intake @15.7psia, vacuum from 10.0 to 13.0psi @14:03:21.78 SS.reconfigure :inhaling 0.91ml/s @14:03:21.83 SS.seek 25ml @14:03:51.73 Stroke completed -- waiting for pressure to equalize ... @14:03:51.75 SS.reconfigure :exhale @14:03:51.79 SS.hold @14:04:06.21 Intake.select :close @14:04:06.37 SSV.dial :exhaust @14:04:07.13 Exhaust.select :open @14:04:07.24 SS.seek 0ml @14:04:16.22 Exhaust.select :close @14:04:16.33 SSV.dial CBV @14:04:16.36
CBV.dial SSV @14:04:17.13 Allowing 130s for 25.0ml of remaining 800.0ml @14:04:17.14 Intake.select :open @14:04:19.30 Intake @15.7psia, vacuum from 10.0 to 13.0psi @14:04:19.32 SS.reconfigure :inhaling 0.91ml/s @14:04:19.37 SS.seek 25ml @14:04:48.75 Stroke completed -- waiting for pressure to equalize ... @14:04:48.76 SS.reconfigure :exhale @14:04:48.81 SS.hold @14:05:05.16 Intake.select :close @14:05:05.32 SSV.dial :exhaust @14:05:06.08 Exhaust.select :open @14:05:06.19 SS.seek 0ml @14:05:15.20 Exhaust.select :close @14:05:15.31 SSV.dial CBV @14:05:15.34
CBV.dial SSV @14:05:16.13 Allowing 130s for 25.0ml of remaining 775.0ml @14:05:16.14 Intake.select :open @14:05:18.30 Intake @15.6psia, vacuum from 10.0 to 13.0psi @14:05:18.32 SS.reconfigure :inhaling 0.91ml/s @14:05:18.37 SS.seek 25ml @14:05:47.75 Stroke completed -- waiting for pressure to equalize ... @14:05:47.76 SS.reconfigure :exhale @14:05:47.80 SS.hold @14:06:04.09 Intake.select :close @14:06:04.25 SSV.dial :exhaust @14:06:05.02 Exhaust.select :open @14:06:05.55 SS.seek 0ml @14:06:14.36 Exhaust.select :close @14:06:14.47 SSV.dial CBV @14:06:14.50
CBV.dial SSV @14:06:15.27 Allowing 130s for 25.0ml of remaining 750.0ml @14:06:15.28 Intake.select :open @14:06:17.45 Intake @15.6psia, vacuum from 10.0 to 13.0psi @14:06:17.46 SS.reconfigure :inhaling 0.91ml/s @14:06:17.51 SS.seek 25ml @14:06:43.04 Vacuum too high -- waiting up to 40 seconds @14:06:43.05 SS.reconfigure :exhale @14:06:43.11 SS.hold @14:06:51.82 Intake @15.5psia, vacuum from 10.0 to 13.0psi @14:06:51.83 SS.reconfigure :inhaling 0.73ml/s @14:06:51.88 SS.seek 25ml @14:06:56.84 Stroke completed -- waiting for pressure to equalize ... @14:06:56.85 SS.reconfigure :exhale @14:06:56.90 SS.hold @14:07:07.93 Intake.select :close @14:07:08.09 SSV.dial :exhaust @14:07:08.87 Exhaust.select :open @14:07:08.99 SS.seek 0ml @14:07:18.00 Exhaust.select :close @14:07:18.11 SSV.dial CBV @14:07:18.14
CBV.dial SSV @14:07:18.91 Allowing 130s for 25.0ml of remaining 725.0ml @14:07:18.92 Intake.select :open @14:07:21.08 Intake @15.5psia, vacuum from 10.0 to 13.0psi @14:07:21.10 SS.reconfigure :inhaling 0.73ml/s @14:07:21.14 SS.seek 25ml @14:07:56.95 Stroke completed -- waiting for pressure to equalize ... @14:07:56.96 SS.reconfigure :exhale @14:07:57.01 SS.hold @14:08:15.46 Intake.select :close @14:08:15.61 SSV.dial :exhaust @14:08:16.37 Exhaust.select :open @14:08:16.49 SS.seek 0ml @14:08:25.31 Exhaust.select :close @14:08:25.42 SSV.dial CBV @14:08:25.46
CBV.dial SSV @14:08:26.20 Allowing 130s for 25.0ml of remaining 700.0ml @14:08:26.21 Intake.select :open @14:08:28.37 Intake @15.6psia, vacuum from 10.0 to 13.0psi @14:08:28.39 SS.reconfigure :inhaling 0.73ml/s @14:08:28.44 SS.seek 25ml @14:09:04.71 Stroke completed -- waiting for pressure to equalize ... @14:09:04.72 SS.reconfigure :exhale @14:09:04.77 SS.hold @14:09:25.16 Intake.select :close @14:09:25.67 SSV.dial :exhaust @14:09:26.50 Exhaust.select :open @14:09:26.61 SS.seek 0ml @14:09:35.36 Exhaust.select :close @14:09:35.47 SSV.dial CBV @14:09:35.49
CBV.dial SSV @14:09:36.28 Allowing 130s for 25.0ml of remaining 675.0ml @14:09:36.29 Intake.select :open @14:09:38.45 Intake @15.5psia, vacuum from 10.0 to 13.0psi @14:09:38.47 SS.reconfigure :inhaling 0.73ml/s @14:09:38.52 SS.seek 25ml @14:10:14.93 Stroke completed -- waiting for pressure to equalize ... @14:10:14.94 SS.reconfigure :exhale @14:10:14.99 SS.hold @14:10:39.38 Intake.select :close @14:10:39.53 SSV.dial :exhaust @14:10:40.29 Exhaust.select :open @14:10:40.40 SS.seek 0ml @14:10:49.78 Exhaust.select :close @14:10:49.89 SSV.dial CBV @14:10:49.92
CBV.dial SSV @14:10:50.70 Allowing 130s for 25.0ml of remaining 650.0ml @14:10:50.71 Intake.select :open @14:10:52.88 Intake @15.4psia, vacuum from 9.9 to 12.9psi @14:10:52.90 SS.reconfigure :inhaling 0.73ml/s @14:10:52.95 SS.seek 25ml @14:11:25.38 Vacuum too high -- waiting up to 40 seconds @14:11:25.39 SS.reconfigure :exhale @14:11:25.44 SS.hold @14:11:40.04 Intake @16.1psia, vacuum from 10.0 to 13.0psi @14:11:40.05 SS.reconfigure :inhaling 0.59ml/s @14:11:40.10 SS.seek 25ml @14:11:44.88 Stroke completed -- waiting for pressure to equalize ... @14:11:44.89 SS.reconfigure :exhale @14:11:44.94 SS.hold @14:11:59.40 Intake.select :close @14:11:59.56 SSV.dial :exhaust @14:12:00.32 Exhaust.select :open @14:12:00.43 SS.seek 0ml @14:12:09.24 Exhaust.select :close @14:12:09.35 SSV.dial CBV @14:12:09.38
CBV.dial SSV @14:12:10.17 Allowing 130s for 25.0ml of remaining 625.0ml @14:12:10.18 Intake.select :open @14:12:12.34 Intake @15.3psia, vacuum from 9.8 to 12.8psi @14:12:12.36 SS.reconfigure :inhaling 0.59ml/s @14:12:12.41 SS.seek 25ml @14:12:57.35 Stroke completed -- waiting for pressure to equalize ... @14:12:57.36 SS.reconfigure :exhale @14:12:57.41 SS.hold @14:13:23.97 Intake.select :close @14:13:24.12 SSV.dial :exhaust @14:13:24.89 Exhaust.select :open @14:13:25.00 SS.seek 0ml @14:13:33.96 Exhaust.select :close @14:13:34.07 SSV.dial CBV @14:13:34.11
CBV.dial SSV @14:13:34.85 Allowing 130s for 25.0ml of remaining 600.0ml @14:13:34.86 Intake.select :open @14:13:37.02 Intake @15.3psia, vacuum from 9.8 to 12.8psi @14:13:37.04 SS.reconfigure :inhaling 0.59ml/s @14:13:37.09 SS.seek 25ml @14:14:21.61 Stroke completed -- waiting for pressure to equalize ... @14:14:21.62 SS.reconfigure :exhale @14:14:21.67 SS.hold @14:14:50.57 Intake.select :close @14:14:50.73 SSV.dial :exhaust @14:14:51.51 Exhaust.select :open @14:14:51.62 SS.seek 0ml @14:15:00.65 Exhaust.select :close @14:15:00.76 SSV.dial CBV @14:15:00.78
CBV.dial SSV @14:15:01.57 Allowing 130s for 25.0ml of remaining 575.0ml @14:15:01.58 Intake.select :open @14:15:03.75 Intake @15.5psia, vacuum from 10.0 to 13.0psi @14:15:03.77 SS.reconfigure :inhaling 0.59ml/s @14:15:03.81 SS.seek 25ml @14:15:42.87 Vacuum too high -- waiting up to 40 seconds @14:15:42.88 SS.reconfigure :exhale @14:15:42.94 SS.hold @14:15:59.41 Intake @15.4psia, vacuum from 9.9 to 12.9psi @14:15:59.42 SS.reconfigure :inhaling 0.47ml/s @14:15:59.47 SS.seek 25ml @14:16:06.13 Stroke completed -- waiting for pressure to equalize ... @14:16:06.14 SS.reconfigure :exhale @14:16:06.18 SS.hold @14:16:24.52 Intake.select :close @14:16:25.11 SSV.dial :exhaust @14:16:25.88 Exhaust.select :open @14:16:25.99 SS.seek 0ml @14:16:34.77 Exhaust.select :close @14:16:34.88 SSV.dial CBV @14:16:34.91
CBV.dial SSV @14:16:35.69 Allowing 130s for 25.0ml of remaining 550.0ml @14:16:35.70 Intake.select :open @14:16:37.87 Intake @15.4psia, vacuum from 9.9 to 12.9psi @14:16:37.88 SS.reconfigure :inhaling 0.47ml/s @14:16:37.93 SS.seek 25ml @14:17:33.53 Stroke completed -- waiting for pressure to equalize ... @14:17:33.54 SS.reconfigure :exhale @14:17:33.59 SS.hold @14:18:04.26 Intake.select :close @14:18:04.41 SSV.dial :exhaust @14:18:05.17 Exhaust.select :open @14:18:05.29 SS.seek 0ml @14:18:14.06 Exhaust.select :close @14:18:14.18 SSV.dial CBV @14:18:14.20
CBV.dial SSV @14:18:14.99 Allowing 130s for 25.0ml of remaining 525.0ml @14:18:15.00 Intake.select :open @14:18:17.16 Intake @15.6psia, vacuum from 10.0 to 13.0psi @14:18:17.18 SS.reconfigure :inhaling 0.47ml/s @14:18:17.23 SS.seek 25ml @14:19:13.01 Stroke completed -- waiting for pressure to equalize ... @14:19:13.02 SS.reconfigure :exhale @14:19:13.07 SS.hold @14:19:49.94 Intake.select :close @14:19:50.09 SSV.dial :exhaust @14:19:50.86 Exhaust.select :open @14:19:50.97 SS.seek 0ml @14:19:59.75 Exhaust.select :close @14:19:59.86 SSV.dial CBV @14:19:59.90
CBV.dial SSV @14:20:00.64 Allowing 130s for 25.0ml of remaining 500.0ml @14:20:00.65 Intake.select :open @14:20:02.81 Intake @15.5psia, vacuum from 10.0 to 13.0psi @14:20:02.83 SS.reconfigure :inhaling 0.47ml/s @14:20:02.88 SS.seek 25ml @14:20:55.33 Vacuum too high -- waiting up to 40 seconds @14:20:55.35 SS.reconfigure :exhale @14:20:55.40 SS.hold @14:21:20.01 Intake @16.7psia, vacuum from 10.0 to 13.0psi @14:21:20.03 SS.reconfigure :inhaling 0.38ml/s @14:21:20.07 SS.seek 25ml @14:21:24.62 Stroke completed -- waiting for pressure to equalize ... @14:21:24.63 SS.reconfigure :exhale @14:21:24.68 SS.hold @14:21:42.93 Intake.select :close @14:21:43.09 SSV.dial :exhaust @14:21:43.86 Exhaust.select :open @14:21:44.40 SS.seek 0ml @14:21:53.25 Exhaust.select :close @14:21:53.36 SSV.dial CBV @14:21:53.38
CBV.dial SSV @14:21:54.17 Allowing 130s for 25.0ml of remaining 475.0ml @14:21:54.18 Intake.select :open @14:21:56.35 Intake @15.4psia, vacuum from 9.9 to 12.9psi @14:21:56.37 SS.reconfigure :inhaling 0.38ml/s @14:21:56.41 SS.seek 25ml @14:23:05.17 Stroke completed -- waiting for pressure to equalize ... @14:23:05.18 SS.reconfigure :exhale @14:23:05.22 SS.hold @14:23:44.48 Intake.select :close @14:23:44.63 SSV.dial :exhaust @14:23:45.43 Exhaust.select :open @14:23:45.54 SS.seek 0ml @14:23:54.68 Exhaust.select :close @14:23:54.79 SSV.dial CBV @14:23:54.81
CBV.dial SSV @14:23:55.60 Allowing 130s for 25.0ml of remaining 450.0ml @14:23:55.62 Intake.select :open @14:23:57.78 Intake @15.5psia, vacuum from 10.0 to 13.0psi @14:23:57.80 SS.reconfigure :inhaling 0.38ml/s @14:23:57.85 SS.seek 25ml @14:25:06.71 Stroke completed -- waiting for pressure to equalize ... @14:25:06.72 SS.reconfigure :exhale @14:25:06.77 SS.hold @14:25:46.95 SS.reconfigure :default @14:25:47.01 SS.hold @14:25:47.07 Intake.select :close @14:25:47.22 ShallowSampler::Clogged in MAIN -- Intake @16psia & Outlet @12psia won't equalize after 575.0ml @14:25:47.24 SSV.dial :exhaust @14:25:48.01 Exhaust.select :open @14:25:48.12 SS.reconfigure :exhale @14:25:48.17 SS.seek 0ml @14:25:57.05 Exhaust.select :close @14:25:57.16 Sampled 575.0ml @14:25:57.18 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @14:25:57.19 Intake.select :close @14:25:57.25 CSV.dial :waste @14:25:57.42 SSV.dial CBV,avoiding: :exhaust @14:25:57.45 CS.seek 0ml @14:25:57.48
CBV.dial SSV @14:25:57.62 CSV.dial :air @14:25:58.36 CS.seek 10ml @14:25:59.37
CTV.dial :air @14:26:00.14 delay 15 seconds @14:26:15.16 SSV.dial :spit,avoiding: :exhaust @14:26:16.48 CTV.dial CSV,avoiding: :intake @14:26:16.52
SS.seek 0ml @14:26:16.64 SSV.dial CBV,avoiding: :exhaust @14:26:23.11 SSV.dial between CBV and plug @14:26:24.32 CBV.dial between SSV and waste2 @14:26:24.72 CTV.dial CSV,avoiding: :air @14:26:24.75
CSV.dial CTV @14:26:25.24 CS.reconfigure :pressurize @14:26:25.29 CS.seek 0ml @14:26:43.72 BubblePoint: Intake @24.3psia, Outlet @0.6psia @14:26:43.73 CBV.dial SSV @14:26:44.05 SS.reconfigure :default @14:26:44.10 SS.seek 10ml @14:26:49.73 delay 15 seconds @14:27:04.86 Evacuated: Intake @23.8psia, Outlet @23.7psia @14:27:04.87 SSV.dial :spit,avoiding: :exhaust @14:27:05.90 SS.reconfigure :exhale @14:27:05.95 SS.seek 0ml @14:27:10.94 SS.reconfigure :fast @14:27:10.99 SS.seek 5ml @14:27:13.79 SSV.dial CBV,avoiding: :exhaust @14:27:13.82
CBV.dial SSV @14:27:15.17 CTV.dial :air,avoiding: :intake @14:27:16.52 CSV.dial CTV @14:27:16.57
SS.seek 10ml @14:27:17.72 CSV.dial :air @14:27:18.16 CS.reconfigure :fast @14:27:18.21 CS.seek 0ml @14:27:19.06
SSV.dial :spit,avoiding: :exhaust @14:27:20.37 SS.reconfigure :exhale @14:27:20.42 SS.seek 0ml @14:27:25.45 SS.reconfigure :fast @14:27:25.50 SS.seek 5ml @14:27:26.26 CS.stop @14:27:28.25 SSV.dial CBV,avoiding: :exhaust @14:27:28.28
CBV.dial SSV @14:27:30.61 SSV.dial :spit,avoiding: :exhaust @14:27:31.92 SS.reconfigure :default @14:27:31.97 SS.seek 0ml @14:27:35.25 SS.stop @14:27:35.29 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @14:27:35.30 Intake.select :close @14:27:35.37 CSV.dial :waste @14:27:35.50 SSV.dial CBV,avoiding: :exhaust @14:27:35.53
CBV.dial SSV @14:27:36.16 CS.seek 0ml @14:27:36.23 CSV.dial :air @14:27:36.90
CTV.dial :air @14:27:36.98 CS.seek 10ml @14:27:37.13
delay 15 seconds @14:27:52.15 SSV.dial :spit,avoiding: :exhaust @14:27:53.48 CTV.dial CSV,avoiding: :intake @14:27:53.53
SS.reconfigure :exhale @14:27:53.61 SS.seek 0ml @14:27:53.67 SSV.dial CBV,avoiding: :exhaust @14:28:01.42 SSV.dial between CBV and plug @14:28:02.27 CBV.dial between SSV and waste2 @14:28:02.73 CSV.dial between air and CTV @14:28:03.04 CS.seek 7.468ml @14:28:09.61 CTV.dial CSV,avoiding: :air @14:28:09.64
CSV.dial CTV @14:28:09.99 CS.reconfigure :pressurize @14:28:10.04 CS.seek 0ml @14:28:25.34 BubblePoint: Intake @24.2psia, Outlet @3.8psia @14:28:25.35 CBV.dial SSV @14:28:25.69 SS.reconfigure :default @14:28:25.74 SS.seek 10ml @14:28:31.19 delay 15 seconds @14:28:46.32 Evacuated: Intake @23.8psia, Outlet @24.0psia @14:28:46.33 SSV.dial :spit,avoiding: :exhaust @14:28:47.37 SS.reconfigure :exhale @14:28:47.42 SS.seek 0ml @14:28:52.40 SS.reconfigure :fast @14:28:52.45 SS.seek 5ml @14:28:55.35 SSV.dial CBV,avoiding: :exhaust @14:28:55.38
CBV.dial SSV @14:28:56.73 CTV.dial :air,avoiding: :intake @14:28:58.06 CSV.dial CTV @14:28:58.10
SS.seek 10ml @14:28:59.32 CSV.dial :air @14:28:59.75 CS.reconfigure :fast @14:28:59.80 CS.seek 0ml @14:29:00.94
SSV.dial :spit,avoiding: :exhaust @14:29:02.26 SS.reconfigure :exhale @14:29:02.31 SS.seek 0ml @14:29:04.54 CS.stop @14:29:07.31
SS.reconfigure :fast @14:29:07.36 SS.seek 5ml @14:29:10.17 SSV.dial CBV,avoiding: :exhaust @14:29:10.19
CBV.dial SSV @14:29:12.54 SSV.dial :spit,avoiding: :exhaust @14:29:13.87 SS.reconfigure :default @14:29:13.92 SS.seek 0ml @14:29:17.16 SS.stop @14:29:17.20 Lysing: Adding 2.0ml AqMeOH @14:29:17.21 CBV.dial between CSV and waste1,avoiding: :DBV @14:29:18.72 CTV.dial CSV @14:29:20.03 CSV.dial CSR @14:29:20.82 Pulling 1ml of CSR.AqMeOH @14:29:20.83 CSR.select :AqMeOH @14:29:20.94 CS.reconfigure :default @14:29:21.41 CS.seek 1ml @14:29:25.05 delay 5 seconds @14:29:30.06 CSR.select :air @14:29:30.24 CS.seek 6ml @14:29:43.83 delay 2 seconds @14:29:45.84 CSV.dial :waste2 @14:29:46.28 CS.reconfigure :fast @14:29:46.33 CS.seek 0ml @14:30:00.70 CSV.dial CSR @14:30:01.15 Pulling 2ml of CSR.AqMeOH @14:30:01.16 CSR.select :AqMeOH @14:30:01.28 CS.reconfigure :default @14:30:01.33 CS.seek 2ml @14:30:07.23 delay 5 seconds @14:30:12.25 CSR.select :air @14:30:12.42 CS.seek 5ml @14:30:20.89 delay 2 seconds @14:30:22.90 CSV.dial CTV @14:30:23.35 CS.seek 1ml @14:30:34.53 Before lysing Collection Puck Heater at 16.1C/30.0psia @14:30:34.55 Heating Puck.from 3, :sh1 in Collection Clamp to 70C for 10:00 @14:30:34.56 CH.seek 70C,8:00 @14:32:41.95 CH.hold 10:00,70C @14:32:42.15 Begun lysing Collection Puck Heater at 68C/37.0psia @14:42:42.16 Ended lysing Collection Puck Heater at 70C/36.4psia @14:42:42.17 CS.seek 0ml @14:42:45.57 CTV.dial between spare and air,avoiding: :intake @14:42:46.60 CSV.dial :air @14:42:47.04 CS.seek 5ml @14:43:00.65 CSV.dial CTV @14:43:01.11 delay 2 seconds @14:43:03.13 CSV.dial :air @14:43:03.58 delay 2 seconds @14:43:05.60 CSV.dial CTV @14:43:06.05 delay 2 seconds @14:43:08.06 CSV.dial :air @14:43:08.52 delay 2 seconds @14:43:10.54 CS.seek 0ml @14:43:24.43 Puck @82.4psia after final lysis pressurization @14:43:24.44 Recovering Lysate into Collection syringe @14:43:24.45 CSV.dial CBV @14:43:25.47 CBV.dial CSV @14:43:25.86 CS.seek 5ml @14:43:39.44 delay 5 seconds @14:43:44.46 CTV.dial :air @14:43:44.99 CS.seek 7ml @14:43:51.32 delay 5 seconds @14:43:56.33 CSV.dial :air2 @14:43:56.79 CS.seek 10ml @14:44:05.22 CSV.dial between air and CTV,avoiding: CBV @14:44:06.38 Lysing Complete @14:44:06.45 Storing sh1 puck in Collection Clamp to tube 2 @14:44:06.62 SC.reconfigure :tight @14:44:06.67 SC.seek 2 @14:44:10.16 SC.configure :limp @14:44:10.38 Elbow.reconfigure :tight @14:44:10.43 Elbow.seek 2 @14:44:12.21 Forearm.reconfigure :tight @14:44:12.26 Forearm.seek 2 + 450 counts @14:44:13.29 SE.reconfigure :pick @14:44:13.35 SE.seek :beamBreak @14:44:15.24 SE.configure :default @14:44:15.40 Elbow.reconfigure :limp @14:44:15.45 Forearm.reconfigure :loose @14:44:15.50 Forearm.seek 2 @14:44:15.82 Elbow.hold @14:44:15.89 Elbow.reconfigure :tight @14:44:15.99 SE.seek 18.12 Pucks below Top Plate @14:44:16.40 Forearm.force -20 @14:44:16.44 Forearm.seek 2 + 266 counts @14:44:16.72 Forearm.reconfigure :tight @14:44:16.77 Forearm.seek 2 + 450 counts @14:44:17.00 SE.seek 19.12 Pucks below Top Plate @14:44:17.66 Forearm.reconfigure :loose @14:44:17.70 Forearm.seek :retract @14:44:18.64 Elbow.seek CC @14:44:20.31 Elbow.reconfigure :loose @14:44:20.41 Forearm.seek CC @14:44:21.42 Hand.close @14:44:22.09 CC.seek :open @14:44:26.87 Forearm.seek :retract @14:44:27.86 Elbow.reconfigure :tight @14:44:27.91 Elbow.seek 2 @14:44:29.67 Elbow.reconfigure :limp @14:44:29.72 Forearm.seek 2 @14:44:30.70 Elbow.hold @14:44:30.76 Elbow.reconfigure :tight @14:44:30.81 Hand.open @14:44:31.58 Forearm.force -20 @14:44:31.62 Forearm.seek 2 + 312 counts @14:44:31.91 Forearm.reconfigure :tight @14:44:31.97 Forearm.seek 2 + 450 counts @14:44:32.23 SE.seek :down @14:44:33.37
Forearm.reconfigure :loose @14:44:33.41 Forearm.seek :retract @14:44:33.71 SE.stop @14:44:34.36
Tranferring DA @14:44:34.37 Beginning samplePassC @14:44:34.38 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:44:34.57 Elbow.seek FlushPuck::Garage @14:44:36.43 Elbow.reconfigure :loose @14:44:36.53 Forearm.seek :garage @14:44:37.54 Hand.close @14:44:38.33 Forearm.seek :retract @14:44:39.36 Elbow.reconfigure :tight @14:44:39.41 Elbow.seek CC @14:44:42.78 Elbow.reconfigure :loose @14:44:42.89 Forearm.reconfigure :tight @14:44:42.94 Forearm.seek CC @14:44:44.22 CC.seek :closed @14:44:48.74 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.33 @14:44:48.81 Hand.open @14:44:49.53 Forearm.reconfigure :loose @14:44:49.57 Forearm.seek :retract @14:44:50.54 PSV.dial PSR @14:44:51.92 Pulling 1.5ml of PSR.flush @14:44:51.93 PSR.select :flush @14:44:52.05 PS.seek 1.5ml @14:45:08.23 delay 5 seconds @14:45:13.24 PSV.dial :waste1 @14:45:13.86 PS.seek 0ml @14:45:30.04 PSV.dial PSR @14:45:30.49 PSR.select :air @14:45:30.61 PS.seek 2.5ml @14:45:56.98 PRV.dial CBV,avoiding: :ab2 @14:45:57.01
CBV.dial PRV @14:45:58.74 PSV.dial :mixloop @14:45:59.79 CTV.dial :air,avoiding: :intake @14:45:59.86 PTV.dial :PRVmixing @14:46:01.20 PS.seek 0ml @14:46:27.50 PSV.dial :air @14:46:27.96 PS.seek 2.5ml @14:46:54.35 PSV.dial :mixloop @14:46:54.82 PS.seek 0ml @14:47:21.16 PSV.dial :air @14:47:22.30 PS.seek 2.5ml @14:47:48.72 PSV.dial :mixloop @14:47:49.18 PS.seek 0ml @14:48:15.47 Garaging FlushPuck in Collection Clamp @14:48:15.55 Elbow.reconfigure :tight @14:48:15.60 Elbow.seek CC @14:48:15.85 Elbow.reconfigure :loose @14:48:15.95 Forearm.seek CC @14:48:17.01 Hand.close @14:48:17.59 CC.seek :open @14:48:22.24 Forearm.seek :retract @14:48:23.20 Elbow.reconfigure :tight @14:48:23.25 Elbow.seek :garage @14:48:26.17 Elbow.reconfigure :loose @14:48:26.27 Forearm.reconfigure :tight @14:48:26.32 Forearm.seek :garage @14:48:27.40 Hand.open @14:48:28.02 Forearm.reconfigure :loose @14:48:28.07 Forearm.seek :retract @14:48:29.07 PSV.dial :mixloop @14:48:29.14 PTV.dial :PRVmixing @14:48:29.22 PRV.dial CBV @14:48:29.25
CBV.dial PRV @14:48:29.47 CTV.dial :air,avoiding: :intake @14:48:29.55 Loading lyfil puck from tube 3 into Collection Clamp @14:48:29.78 SC.reconfigure :tight @14:48:29.83 SC.seek 3 @14:48:33.41 SC.configure :limp @14:48:33.62 Elbow.reconfigure :tight @14:48:33.67 Elbow.seek 3 @14:48:35.60 Forearm.reconfigure :tight @14:48:36.06 Forearm.seek 3 + 450 counts @14:48:37.12 SE.reconfigure :pick @14:48:37.18 SE.seek :beamBreak @14:48:39.57 SE.configure :default @14:48:39.73 Elbow.seek 3 @14:48:39.93 Forearm.seek 3 + 300 counts @14:48:40.18 Forearm.reconfigure :loose @14:48:40.23 Hand.close @14:48:40.85 SE.seek :down @14:48:41.05
Forearm.seek :retract @14:48:42.01 Elbow.seek CC @14:48:42.83 SE.stop @14:48:43.56
Elbow.reconfigure :loose @14:48:43.67 Forearm.reconfigure :tight @14:48:43.71 Forearm.seek CC @14:48:45.02 CC.seek :closed @14:48:49.53 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.15 @14:48:49.54 Hand.open @14:48:50.27 Forearm.reconfigure :loose @14:48:50.31 Forearm.seek :retract @14:48:51.29 CTV.dial CSV,avoiding: :intake @14:48:52.65 CSV.dial CTV @14:48:52.99 PS.seek 2.5ml @14:49:19.95 CS.seek 4.5ml @14:49:34.90 delay 8 seconds @14:49:42.92 PSV.dial :air @14:49:43.53 delay 2 seconds @14:49:45.55 PSV.dial :waste2 @14:49:46.32 PS.seek 0ml @14:50:12.66 CBV.dial between PRV and waste1 @14:50:12.96 CSV.dial :air @14:50:13.55 CS.seek 10ml @14:50:28.49 CSV.dial CTV @14:50:28.94 CS.seek 0ml @14:50:55.55 CBV.dial :waste1 @14:50:55.87 delay 5 seconds @14:51:00.89 CTV.dial :air,avoiding: :intake @14:51:02.29 Storing lyfil puck in Collection Clamp to tube 2 @14:51:02.46 SC.reconfigure :tight @14:51:02.51 SC.seek 2 @14:51:06.11 SC.configure :limp @14:51:06.33 Elbow.reconfigure :tight @14:51:06.38 Elbow.seek 2 @14:51:08.19 Forearm.reconfigure :tight @14:51:08.24 Forearm.seek 2 + 450 counts @14:51:09.27 SE.reconfigure :pick @14:51:09.33 SE.seek :beamBreak @14:51:11.23 SE.configure :default @14:51:11.40 Elbow.reconfigure :limp @14:51:11.45 Forearm.reconfigure :loose @14:51:11.49 Forearm.seek 2 @14:51:11.71 Elbow.hold @14:51:11.77 Elbow.reconfigure :tight @14:51:11.87 SE.seek 19.11 Pucks below Top Plate @14:51:12.34 Forearm.force -20 @14:51:12.39 Forearm.seek 2 + 315 counts @14:51:13.01 Forearm.reconfigure :tight @14:51:13.06 Forearm.seek 2 + 450 counts @14:51:13.29 SE.seek 20.11 Pucks below Top Plate @14:51:13.84 Forearm.reconfigure :loose @14:51:13.89 Forearm.seek :retract @14:51:14.84 Elbow.seek CC @14:51:16.54 Elbow.reconfigure :loose @14:51:16.64 Forearm.seek CC @14:51:17.63 Hand.close @14:51:18.21 CC.seek :open @14:51:22.94 Forearm.seek :retract @14:51:23.91 Elbow.reconfigure :tight @14:51:23.96 Elbow.seek 2 @14:51:25.74 Elbow.reconfigure :limp @14:51:25.80 Forearm.seek 2 @14:51:26.79 Elbow.hold @14:51:26.85 Elbow.reconfigure :tight @14:51:26.90 Hand.open @14:51:27.58 Forearm.force -20 @14:51:27.62 Forearm.seek 2 + 317 counts @14:51:28.00 Forearm.reconfigure :tight @14:51:28.05 Forearm.seek 2 + 450 counts @14:51:28.38 SE.seek :down @14:51:29.52
Forearm.reconfigure :loose @14:51:29.56 Forearm.seek :retract @14:51:30.06 SE.stop @14:51:30.99
PSV.dial :air @14:51:31.74 PS.seek 2.5ml @14:51:58.09 delay 1 second @14:51:59.11 PSV.dial :mixloop @14:51:59.56 delay 1 second @14:52:00.58 PSV.dial :air @14:52:01.05 delay 1 second @14:52:02.06 PSV.dial :mixloop @14:52:02.53 delay 1 second @14:52:03.55 PSV.dial :air @14:52:04.02 delay 1 second @14:52:05.04 PS.seek 0ml @14:52:31.31 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @14:52:31.50 Elbow.seek FlushPuck::Garage @14:52:33.33 Elbow.reconfigure :loose @14:52:33.43 Forearm.seek :garage @14:52:34.47 Hand.close @14:52:35.20 Forearm.seek :retract @14:52:36.18 Elbow.reconfigure :tight @14:52:36.23 Elbow.seek PC @14:52:37.48 Elbow.reconfigure :loose @14:52:37.58 Forearm.reconfigure :tight @14:52:37.63 Forearm.seek PC @14:52:38.93 PC.seek :closed @14:52:43.57 Processing Clamp onSpring/offSpring for 16/26 samples, on/off torque 1.59 @14:52:43.65 Hand.open @14:52:44.27 Forearm.reconfigure :loose @14:52:44.77 Forearm.seek :retract @14:52:45.80 PRV.dial :air @14:52:46.25 PSV.dial :mixloop @14:52:46.78 PS.seek 0.3ml @14:52:50.65 PSV.dial PSR @14:52:51.70 Pulling 0.4ml of PSR.air @14:52:51.71 PSR.select :air @14:52:51.73 PS.seek 0.7ml @14:52:56.59 delay 3 seconds @14:52:59.61 PSR.select :air @14:52:59.63 PS.seek 2.5ml @14:53:18.90 delay 3 seconds @14:53:21.92 PSV.dial :mixloop @14:53:22.97 PTV.dial :mixing @14:53:23.73 PBV.dial :waste2 @14:53:24.69 PS.seek 0ml @14:53:51.06 Garaging FlushPuck in Processing Clamp @14:53:51.13 Elbow.reconfigure :tight @14:53:51.18 Elbow.seek PC @14:53:51.53 Elbow.reconfigure :loose @14:53:51.63 Forearm.seek PC @14:53:52.60 Hand.close @14:53:53.21 PC.seek :open @14:53:57.92 Forearm.seek :retract @14:53:58.91 Elbow.reconfigure :tight @14:53:58.96 Elbow.seek :garage @14:54:00.26 Elbow.reconfigure :loose @14:54:00.37 Forearm.reconfigure :tight @14:54:00.41 Forearm.seek :garage @14:54:01.36 Hand.open @14:54:02.02 Forearm.reconfigure :loose @14:54:02.07 Forearm.seek :retract @14:54:03.04 Loading da puck from tube 3 into Processing Clamp @14:54:03.28 SC.reconfigure :tight @14:54:03.33 SC.seek 3 @14:54:06.86 SC.configure :limp @14:54:07.49 Elbow.reconfigure :tight @14:54:07.56 Elbow.seek 3 @14:54:09.42 Forearm.reconfigure :tight @14:54:09.47 Forearm.seek 3 + 450 counts @14:54:10.57 SE.reconfigure :pick @14:54:10.63 SE.seek :beamBreak @14:54:13.06 SE.configure :default @14:54:13.22 Elbow.seek 3 @14:54:13.41 Forearm.seek 3 + 300 counts @14:54:13.66 Forearm.reconfigure :loose @14:54:13.71 Hand.close @14:54:14.35 SE.seek :down @14:54:14.56
Forearm.seek :retract @14:54:15.51 Elbow.seek PC @14:54:16.39 SE.stop @14:54:18.31
Elbow.reconfigure :loose @14:54:18.42 Forearm.reconfigure :tight @14:54:18.46 Forearm.seek PC @14:54:19.71 PC.seek :closed @14:54:24.37 Processing Clamp onSpring/offSpring for 16/26 samples, on/off torque 1.63 @14:54:24.38 Hand.open @14:54:25.03 Forearm.reconfigure :loose @14:54:25.07 Forearm.seek :retract @14:54:26.03 Heating Puck.from 3, :da in Processing Clamp to 28C for 2:00:00 @14:54:26.04 PH.seek 28C,5:00 @14:55:03.13 PH.hold 2:00:00,28C @14:55:03.18 delay 1:30 @14:56:33.21 PSR.select :air @14:56:33.23 PSV.dial PSR @14:56:34.27 Pulling 0.5ml of PSR.wash @14:56:34.28 PSR.select :wash @14:56:34.41 PS.seek 0.5ml @14:56:40.37 delay 5 seconds @14:56:45.39 PSR.select :air @14:56:45.52 PS.seek 2ml @14:57:01.58 PTV.dial :puck @14:57:02.34 PSV.dial PTV @14:57:03.10 PBV.dial :waste1 @14:57:04.60 PS.seek 1.5ml @14:57:10.65 delay 3 seconds @14:57:13.67 PBV.dial between PSV and waste1 @14:57:14.05 PS.seek 0ml @14:57:30.21 delay 5:00 @15:02:30.23 recoverPSRreagent08 @15:02:30.24 PTV.dial :puck @15:02:30.42 PBV.dial :waste1 @15:02:30.80 delay 30 seconds @15:03:00.82 PBV.dial between PSV and waste1 @15:03:01.25 PSR.select :air @15:03:01.27 PSV.dial PSR @15:03:02.04 PS.reconfigure :fast @15:03:02.09 PS.seek 2.5ml @15:03:25.89 PSV.dial PTV @15:03:26.66 delay 5 seconds @15:03:31.68 PSV.dial PSR @15:03:32.55 delay 5 seconds @15:03:37.57 PSV.dial PTV @15:03:38.42 delay 5 seconds @15:03:43.44 PSV.dial :air @15:03:44.23 PS.seek 0ml @15:04:07.55 PTV.dial :PRVmixing,via: PRV @15:04:08.89 PSV.dial :mixloop @15:04:09.36 PRV.dial :ab1 @15:04:10.10 PS.reconfigure :slow @15:04:10.15 PS.seek 0.15ml @15:04:13.67 delay 2 seconds @15:04:15.69 CBV.dial PRV @15:04:15.72
PRV.dial CBV @15:04:16.28 PS.seek 0.35ml @15:04:20.89 delay 2 seconds @15:04:22.91 CBV.dial between PRV and PSV @15:04:23.21 PRV.dial :air @15:04:23.68 PS.seek 0.9ml @15:04:35.42 PRV.dial :waste2 @15:04:36.18 PS.seek 0.5ml @15:04:44.89 addPMLReagent08 @15:04:44.90 PSV.dial :air @15:04:45.36 PS.reconfigure :default @15:04:45.41 PS.seek 2ml @15:05:01.51 PSV.dial :PML @15:05:01.98 PTV.dial :mixing @15:05:02.73 PBV.dial :waste1 @15:05:03.15 PS.seek 1.85ml @15:05:05.54 PBV.dial between PSV and waste1 @15:05:05.91 PS.seek 0ml @15:05:25.83 delay 2 seconds @15:05:27.85 Beginning samplePassC @15:05:27.86 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @15:05:28.05 Elbow.reconfigure :tight @15:05:28.09 Elbow.seek FlushPuck::Garage @15:05:29.38 Elbow.reconfigure :loose @15:05:29.48 Forearm.seek :garage @15:05:30.88 Hand.close @15:05:31.57 Forearm.seek :retract @15:05:32.61 Elbow.reconfigure :tight @15:05:32.65 Elbow.seek CC @15:05:35.52 Elbow.reconfigure :loose @15:05:35.63 Forearm.reconfigure :tight @15:05:35.67 Forearm.seek CC @15:05:36.94 CC.seek :closed @15:05:41.49 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.47 @15:05:41.57 Hand.open @15:05:42.26 Forearm.reconfigure :loose @15:05:42.31 Forearm.seek :retract @15:05:43.27 PSV.dial PSR @15:05:44.37 Pulling 1.5ml of PSR.flush @15:05:44.38 PSR.select :flush @15:05:44.50 PS.seek 1.5ml @15:06:00.70 delay 5 seconds @15:06:05.72 PSV.dial :waste1 @15:06:06.19 PS.seek 0ml @15:06:22.39 PSV.dial PSR @15:06:22.86 PSR.select :air @15:06:22.98 PS.seek 2.5ml @15:06:49.30 PRV.dial CBV,avoiding: :ab2 @15:06:49.33
CBV.dial PRV @15:06:50.95 PSV.dial :mixloop @15:06:52.00 CTV.dial :air,avoiding: :intake @15:06:52.07 PTV.dial :PRVmixing @15:06:52.85 PS.seek 0ml @15:07:19.20 PSV.dial :air @15:07:19.67 PS.seek 2.5ml @15:07:46.04 PSV.dial :mixloop @15:07:46.53 PS.seek 0ml @15:08:13.04 PSV.dial :air @15:08:13.52 PS.seek 2.5ml @15:08:39.88 PSV.dial :mixloop @15:08:40.36 PS.seek 0ml @15:09:06.73 Garaging FlushPuck in Collection Clamp @15:09:06.80 Elbow.reconfigure :tight @15:09:06.86 Elbow.seek CC @15:09:07.17 Elbow.reconfigure :loose @15:09:07.28 Forearm.seek CC @15:09:08.32 Hand.close @15:09:08.95 CC.seek :open @15:09:13.60 Forearm.seek :retract @15:09:14.65 Elbow.reconfigure :tight @15:09:14.70 Elbow.seek :garage @15:09:17.50 Elbow.reconfigure :loose @15:09:17.60 Forearm.reconfigure :tight @15:09:17.65 Forearm.seek :garage @15:09:18.61 Hand.open @15:09:19.26 Forearm.reconfigure :loose @15:09:19.31 Forearm.seek :retract @15:09:20.28 delayUntil "15:13:27.83" @15:09:20.30 Whole Cell Archive sampling 1000ml @15:09:20.32 Loading wcr puck from tube 3 into Collection Clamp @15:09:20.57 SC.reconfigure :tight @15:09:20.62 SC.seek 3 @15:09:20.73 SC.configure :limp @15:09:20.96 Elbow.reconfigure :tight @15:09:21.01 Elbow.seek 3 @15:09:23.37 Forearm.reconfigure :tight @15:09:23.43 Forearm.seek 3 + 450 counts @15:09:24.45 SE.reconfigure :pick @15:09:24.51 SE.seek :beamBreak @15:09:26.93 SE.configure :default @15:09:27.09 Elbow.seek 3 @15:09:27.29 Forearm.seek 3 + 300 counts @15:09:27.47 Forearm.reconfigure :loose @15:09:27.52 Hand.close @15:09:28.21 SE.seek :down @15:09:28.42 Forearm.seek :retract @15:09:29.39 Elbow.seek CC @15:09:30.33 SE.stop @15:09:31.05 Elbow.reconfigure :loose @15:09:31.16 Forearm.reconfigure :tight @15:09:31.21 Forearm.seek CC @15:09:32.52 CC.seek :closed @15:09:37.04 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.33 @15:09:37.06 Hand.open @15:09:37.76 Forearm.reconfigure :loose @15:09:37.81 Forearm.seek :retract @15:09:39.24 collectSample 1000ml w/bubble point of 35psi @15:09:39.26 Priming 7ml @15:09:39.27 Intake.select :close @15:09:39.28 Exhaust.select :close @15:09:39.29 SS.hold @15:09:39.52 Calibrating pressure sensors @15:09:39.53 CTV.dial :air @15:09:39.60 SSV.dial :spit,avoiding: :exhaust @15:09:39.70 SS.reconfigure :fast @15:09:39.75 SS.seek 5ml @15:09:42.43 SSV.dial CBV @15:09:42.46 CBV.dial SSV @15:09:43.83 delay 3.8 seconds @15:09:47.76 Sensor limits: Intake max 108.9psia; Outlet max 100.8psia @15:09:47.78 SSV.dial :spit @15:09:49.10 SS.seek 0ml @15:09:51.96 SSV.dial CBV @15:09:53.29 CTV.dial :intake @15:09:54.06 SSV.dial CBV @15:09:54.09 CBV.dial SSV @15:09:54.24 Allowing 40s for 7.0ml of remaining 7.0ml @15:09:54.26 Intake.select :open @15:09:56.42 Intake @14.7psia, vacuum from 9.2 to 12.2psi @15:09:56.44 SS.reconfigure :inhaling 2.20ml/s @15:09:56.49 SS.seek 7ml @15:10:03.00 Stroke completed -- waiting for pressure to equalize ... @15:10:03.01 SS.reconfigure :exhale @15:10:03.06 SS.hold @15:10:15.74 Intake.select :close @15:10:15.90 SSV.dial :exhaust @15:10:16.67 Exhaust.select :open @15:10:16.79 SS.seek 0ml @15:10:20.80 Exhaust.select :close @15:10:20.91 Sampling 1000.0ml @15:10:20.92 Intake.select :close @15:10:20.93 Exhaust.select :close @15:10:20.95 SS.reconfigure :default @15:10:20.99 SS.hold @15:10:21.20 CTV.dial :intake @15:10:21.29 SSV.dial CBV @15:10:21.33 CBV.dial SSV @15:10:22.10 Allowing 130s for 25.0ml of remaining 1000.0ml @15:10:22.11 Intake.select :open @15:10:24.28 Intake @15.4psia, vacuum from 9.9 to 12.9psi @15:10:24.30 SS.reconfigure :inhaling 2.20ml/s @15:10:24.35 SS.seek 25ml @15:10:33.49 Vacuum too high -- waiting up to 40 seconds @15:10:33.51 SS.reconfigure :exhale @15:10:33.56 SS.hold @15:10:40.48 Intake @15.2psia, vacuum from 9.7 to 12.7psi @15:10:40.50 SS.reconfigure :inhaling 1.76ml/s @15:10:40.55 SS.seek 25ml @15:10:47.87 Stroke completed -- waiting for pressure to equalize ... @15:10:47.89 SS.reconfigure :exhale @15:10:47.94 SS.hold @15:11:16.62 Intake.select :close @15:11:16.78 SSV.dial :exhaust @15:11:17.55 Exhaust.select :open @15:11:17.66 SS.seek 0ml @15:11:26.52 Exhaust.select :close @15:11:26.63 SSV.dial CBV @15:11:26.65 CBV.dial SSV @15:11:27.42 Allowing 130s for 25.0ml of remaining 975.0ml @15:11:27.44 Intake.select :open @15:11:29.61 Intake @15.8psia, vacuum from 10.0 to 13.0psi @15:11:29.63 SS.reconfigure :inhaling 1.76ml/s @15:11:29.67 SS.seek 25ml @15:11:39.71 Vacuum too high -- waiting up to 40 seconds @15:11:39.73 SS.reconfigure :exhale @15:11:39.78 SS.hold @15:11:46.13 Intake @17.9psia, vacuum from 10.0 to 13.0psi @15:11:46.15 SS.reconfigure :inhaling 1.41ml/s @15:11:46.20 SS.seek 25ml @15:11:50.03 Vacuum too high -- waiting up to 40 seconds @15:11:50.05 SS.reconfigure :exhale @15:11:50.10 SS.hold @15:11:53.23 Intake @17.7psia, vacuum from 10.0 to 13.0psi @15:11:53.25 SS.reconfigure :inhaling 1.13ml/s @15:11:53.30 SS.seek 25ml @15:11:59.65 Vacuum too high -- waiting up to 40 seconds @15:11:59.66 SS.reconfigure :exhale @15:11:59.72 SS.hold @15:12:02.81 Intake @18.0psia, vacuum from 10.0 to 13.0psi @15:12:02.83 SS.reconfigure :inhaling 0.91ml/s @15:12:02.88 SS.seek 25ml @15:12:04.28 Stroke completed -- waiting for pressure to equalize ... @15:12:04.29 SS.reconfigure :exhale @15:12:04.34 SS.hold @15:12:23.01 Intake.select :close @15:12:23.17 SSV.dial :exhaust @15:12:23.94 Exhaust.select :open @15:12:24.05 SS.seek 0ml @15:12:32.80 Exhaust.select :close @15:12:32.91 SSV.dial CBV @15:12:32.94 CBV.dial SSV @15:12:33.72 Allowing 130s for 25.0ml of remaining 950.0ml @15:12:33.73 Intake.select :open @15:12:35.90 Intake @16.0psia, vacuum from 10.0 to 13.0psi @15:12:35.92 SS.reconfigure :inhaling 0.91ml/s @15:12:35.97 SS.seek 25ml @15:13:06.00 Stroke completed -- waiting for pressure to equalize ... @15:13:06.01 SS.reconfigure :exhale @15:13:06.06 SS.hold @15:13:26.75 Intake.select :close @15:13:26.90 SSV.dial :exhaust @15:13:27.69 Exhaust.select :open @15:13:27.81 SS.seek 0ml @15:13:27.86 recoverPRVreagent08 @15:13:27.87 PTV.dial :mixing @15:13:28.63 PBV.dial :waste1 @15:13:29.01 delay 30 seconds @15:13:36.61 Exhaust.select :close @15:13:37.17 SSV.dial CBV @15:13:37.20 CBV.dial SSV @15:13:37.97 Allowing 130s for 25.0ml of remaining 925.0ml @15:13:37.98 Intake.select :open @15:13:40.15 Intake @16.0psia, vacuum from 10.0 to 13.0psi @15:13:40.17 SS.reconfigure :inhaling 0.91ml/s @15:13:40.22 SS.seek 25ml @15:13:59.03 PBV.dial between PSV and waste1 @15:13:59.41 PSV.dial :air @15:13:59.88 PS.reconfigure :fast @15:13:59.93 PS.seek 2.5ml @15:14:10.14 Stroke completed -- waiting for pressure to equalize ... @15:14:10.15 SS.reconfigure :exhale @15:14:10.20 SS.hold @15:14:24.09 PSV.dial :mixloop @15:14:24.62 delay 2 seconds @15:14:26.97 PSV.dial :air @15:14:27.44 delay 2 seconds @15:14:28.57 Intake.select :close @15:14:28.74 SSV.dial :exhaust @15:14:29.46 PSV.dial :mixloop @15:14:29.52 Exhaust.select :open @15:14:29.66 SS.seek 0ml @15:14:29.97 delay 2 seconds @15:14:31.99 PSV.dial :air @15:14:32.46 PS.seek 0ml @15:14:38.68 Exhaust.select :close @15:14:38.80 SSV.dial CBV @15:14:38.84 CBV.dial SSV @15:14:39.59 Allowing 130s for 25.0ml of remaining 900.0ml @15:14:39.61 Intake.select :open @15:14:41.77 Intake @16.0psia, vacuum from 10.0 to 13.0psi @15:14:41.79 SS.reconfigure :inhaling 0.91ml/s @15:14:41.84 SS.seek 25ml @15:14:56.13 washCycleDA #1 of 2 @15:14:56.14 PSR.select :air @15:14:56.16 PSV.dial PSR @15:14:57.50 Pulling 0.5ml of PSR.wash @15:14:57.51 PSR.select :wash @15:14:57.63 PS.reconfigure :default @15:14:57.68 PS.seek 0.5ml @15:15:03.66 delay 5 seconds @15:15:08.68 PSR.select :air @15:15:08.80 PS.seek 2ml @15:15:11.42 Stroke completed -- waiting for pressure to equalize ... @15:15:11.43 SS.reconfigure :exhale @15:15:11.48 SS.hold @15:15:24.97 PTV.dial :puck @15:15:25.70 PSV.dial PTV @15:15:26.45 PBV.dial :waste1 @15:15:26.89 PS.seek 1.5ml @15:15:29.94 Intake.select :close @15:15:30.09 SSV.dial :exhaust @15:15:30.86 Exhaust.select :open @15:15:30.97 SS.seek 0ml @15:15:32.81 delay 3 seconds @15:15:35.83 PBV.dial between PSV and waste1 @15:15:36.22 PS.seek 0ml @15:15:39.95 Exhaust.select :close @15:15:40.06 SSV.dial CBV @15:15:40.09 CBV.dial SSV @15:15:40.86 Allowing 130s for 25.0ml of remaining 875.0ml @15:15:40.88 Intake.select :open @15:15:43.04 Intake @15.9psia, vacuum from 10.0 to 13.0psi @15:15:43.06 SS.reconfigure :inhaling 0.91ml/s @15:15:43.11 SS.seek 25ml @15:15:52.38 delay 2:00 @15:16:12.63 Stroke completed -- waiting for pressure to equalize ... @15:16:12.65 SS.reconfigure :exhale @15:16:12.69 SS.hold @15:16:31.52 Intake.select :close @15:16:31.69 SSV.dial :exhaust @15:16:32.46 Exhaust.select :open @15:16:32.58 SS.seek 0ml @15:16:41.60 Exhaust.select :close @15:16:41.72 SSV.dial CBV @15:16:41.76 CBV.dial SSV @15:16:42.53 Allowing 130s for 25.0ml of remaining 850.0ml @15:16:42.54 Intake.select :open @15:16:44.70 Intake @15.8psia, vacuum from 10.0 to 13.0psi @15:16:44.72 SS.reconfigure :inhaling 0.91ml/s @15:16:44.77 SS.seek 25ml @15:17:14.07 Stroke completed -- waiting for pressure to equalize ... @15:17:14.08 SS.reconfigure :exhale @15:17:14.13 SS.hold @15:17:30.75 Intake.select :close @15:17:30.91 SSV.dial :exhaust @15:17:31.68 Exhaust.select :open @15:17:31.80 SS.seek 0ml @15:17:40.73 Exhaust.select :close @15:17:40.84 SSV.dial CBV @15:17:40.87 CBV.dial SSV @15:17:41.64 Allowing 130s for 25.0ml of remaining 825.0ml @15:17:41.65 Intake.select :open @15:17:43.81 Intake @15.9psia, vacuum from 10.0 to 13.0psi @15:17:43.83 SS.reconfigure :inhaling 0.91ml/s @15:17:43.88 SS.seek 25ml @15:17:52.40 recoverPSRreagent08 @15:17:52.41 PTV.dial :puck @15:17:52.43 PBV.dial :waste1 @15:17:52.80 delay 30 seconds @15:18:10.08 Vacuum too high -- waiting up to 40 seconds @15:18:10.10 SS.reconfigure :exhale @15:18:10.15 SS.hold @15:18:17.23 Intake @16.0psia, vacuum from 10.0 to 13.0psi @15:18:17.25 SS.reconfigure :inhaling 0.73ml/s @15:18:17.30 SS.seek 25ml @15:18:21.51 Stroke completed -- waiting for pressure to equalize ... @15:18:21.52 SS.reconfigure :exhale @15:18:21.57 SS.hold @15:18:22.84 PBV.dial between PSV and waste1 @15:18:23.22 PSR.select :air @15:18:23.24 PSV.dial PSR @15:18:24.04 PS.reconfigure :fast @15:18:24.15 PS.seek 2.5ml @15:18:31.37 Intake.select :close @15:18:31.53 SSV.dial :exhaust @15:18:32.32 Exhaust.select :open @15:18:32.43 SS.seek 0ml @15:18:41.23 Exhaust.select :close @15:18:41.34 SSV.dial CBV @15:18:41.37 CBV.dial SSV @15:18:42.13 Allowing 130s for 25.0ml of remaining 800.0ml @15:18:42.15 Intake.select :open @15:18:44.32 Intake @15.8psia, vacuum from 10.0 to 13.0psi @15:18:44.34 SS.reconfigure :inhaling 0.73ml/s @15:18:44.38 SS.seek 25ml @15:18:48.06 PSV.dial PTV @15:18:48.84 delay 5 seconds @15:18:53.86 PSV.dial PSR @15:18:54.76 delay 5 seconds @15:18:59.78 PSV.dial PTV @15:19:00.53 delay 5 seconds @15:19:05.55 PSV.dial :air @15:19:06.32 PS.seek 0ml @15:19:20.55 Stroke completed -- waiting for pressure to equalize ... @15:19:20.57 SS.reconfigure :exhale @15:19:20.61 SS.hold @15:19:29.78 washCycleDA #2 of 2 @15:19:29.79 PSR.select :air @15:19:29.81 PSV.dial PSR @15:19:31.14 Pulling 0.5ml of PSR.wash @15:19:31.15 PSR.select :wash @15:19:31.27 PS.reconfigure :default @15:19:31.32 PS.seek 0.5ml @15:19:36.97 Intake.select :close @15:19:37.14 SSV.dial :exhaust @15:19:37.25 delay 5 seconds @15:19:37.91 Exhaust.select :open @15:19:38.03 SS.seek 0ml @15:19:42.26 PSR.select :air @15:19:42.38 PS.seek 2ml @15:19:46.71 Exhaust.select :close @15:19:46.82 SSV.dial CBV @15:19:46.85 CBV.dial SSV @15:19:47.62 Allowing 130s for 25.0ml of remaining 775.0ml @15:19:47.63 Intake.select :open @15:19:49.79 Intake @15.7psia, vacuum from 10.0 to 13.0psi @15:19:49.81 SS.reconfigure :inhaling 0.73ml/s @15:19:49.86 SS.seek 25ml @15:19:58.59 PTV.dial :puck @15:19:58.60 PSV.dial PTV @15:19:59.36 PBV.dial :waste1 @15:19:59.79 PS.seek 1.5ml @15:20:05.78 delay 3 seconds @15:20:08.80 PBV.dial between PSV and waste1 @15:20:09.17 PS.seek 0ml @15:20:25.25 delay 2:00 @15:20:26.16 Stroke completed -- waiting for pressure to equalize ... @15:20:26.55 SS.reconfigure :exhale @15:20:26.67 SS.hold @15:20:45.05 Intake.select :close @15:20:45.21 SSV.dial :exhaust @15:20:45.97 Exhaust.select :open @15:20:46.09 SS.seek 0ml @15:20:54.82 Exhaust.select :close @15:20:54.93 SSV.dial CBV @15:20:54.97 CBV.dial SSV @15:20:55.72 Allowing 130s for 25.0ml of remaining 750.0ml @15:20:55.74 Intake.select :open @15:20:58.36 Intake @15.7psia, vacuum from 10.0 to 13.0psi @15:20:58.38 SS.reconfigure :inhaling 0.73ml/s @15:20:58.43 SS.seek 25ml @15:21:34.85 Stroke completed -- waiting for pressure to equalize ... @15:21:34.86 SS.reconfigure :exhale @15:21:34.91 SS.hold @15:21:55.35 Intake.select :close @15:21:55.51 SSV.dial :exhaust @15:21:56.29 Exhaust.select :open @15:21:56.87 SS.seek 0ml @15:22:05.74 Exhaust.select :close @15:22:05.85 SSV.dial CBV @15:22:05.88 CBV.dial SSV @15:22:06.66 Allowing 130s for 25.0ml of remaining 725.0ml @15:22:06.68 Intake.select :open @15:22:08.84 Intake @15.8psia, vacuum from 10.0 to 13.0psi @15:22:08.86 SS.reconfigure :inhaling 0.73ml/s @15:22:08.91 SS.seek 25ml @15:22:25.27 recoverPSRreagent08 @15:22:25.28 PTV.dial :puck @15:22:25.29 PBV.dial :waste1 @15:22:25.67 delay 30 seconds @15:22:41.76 Vacuum too high -- waiting up to 40 seconds @15:22:41.78 SS.reconfigure :exhale @15:22:41.83 SS.hold @15:22:53.12 Intake @16.0psia, vacuum from 10.0 to 13.0psi @15:22:53.14 SS.reconfigure :inhaling 0.59ml/s @15:22:53.19 SS.seek 25ml @15:22:55.69 PBV.dial between PSV and waste1 @15:22:56.04 PSR.select :air @15:22:56.45 PSV.dial PSR @15:22:57.28 PS.reconfigure :fast @15:22:57.33 PS.seek 2.5ml @15:22:58.04 Stroke completed -- waiting for pressure to equalize ... @15:22:58.05 SS.reconfigure :exhale @15:22:58.10 SS.hold @15:23:10.79 Intake.select :close @15:23:10.95 SSV.dial :exhaust @15:23:11.72 Exhaust.select :open @15:23:11.84 SS.seek 0ml @15:23:20.70 Exhaust.select :close @15:23:20.82 SSV.dial CBV @15:23:20.86 CBV.dial SSV @15:23:21.33 PSV.dial PTV @15:23:21.63 Allowing 130s for 25.0ml of remaining 700.0ml @15:23:21.64 Intake.select :open @15:23:22.12 delay 5 seconds @15:23:24.26 Intake @15.6psia, vacuum from 10.0 to 13.0psi @15:23:24.28 SS.reconfigure :inhaling 0.59ml/s @15:23:24.33 SS.seek 25ml @15:23:27.14 PSV.dial PSR @15:23:27.90 delay 5 seconds @15:23:32.92 PSV.dial PTV @15:23:33.70 delay 5 seconds @15:23:38.72 PSV.dial :air @15:23:39.49 PS.seek 0ml @15:24:02.93 getPMLReagent08 @15:24:02.94 PRV.dial :air @15:24:03.41 delay 5 seconds @15:24:08.43 PTV.dial :PRVmixing @15:24:09.01 Stroke completed -- waiting for pressure to equalize ... @15:24:09.03 SS.reconfigure :exhale @15:24:09.08 SS.hold @15:24:09.76 delay 5 seconds @15:24:14.77 PRV.dial :ab2 @15:24:15.24 PTV.dial :PRVmixing @15:24:15.32 PSV.dial :mixloop @15:24:15.79 PS.reconfigure :default @15:24:15.84 PS.seek 0.3ml @15:24:19.68 delay 2 seconds @15:24:22.22 PRV.dial :air @15:24:22.77 PS.seek 0.55ml @15:24:26.21 addPMLReagent08 @15:24:26.22 PSV.dial :air @15:24:26.70 PS.seek 2ml @15:24:32.06 Intake.select :close @15:24:32.22 SSV.dial :exhaust @15:24:33.00 Exhaust.select :open @15:24:33.12 SS.seek 0ml @15:24:42.12 Exhaust.select :close @15:24:42.24 SSV.dial CBV @15:24:42.28 CBV.dial SSV @15:24:42.31 PSV.dial :PML @15:24:42.82 PTV.dial :mixing @15:24:43.08 Allowing 130s for 25.0ml of remaining 675.0ml @15:24:43.09 Intake.select :open @15:24:43.58 PBV.dial :waste1 @15:24:44.01 PS.seek 1.85ml @15:24:45.26 Intake @15.6psia, vacuum from 10.0 to 13.0psi @15:24:45.28 SS.reconfigure :inhaling 0.59ml/s @15:24:45.33 SS.seek 25ml @15:24:46.40 PBV.dial between PSV and waste1 @15:24:46.76 PS.seek 0ml @15:25:06.58 delay 2 seconds @15:25:08.60 delay 8:00 @15:25:29.89 Stroke completed -- waiting for pressure to equalize ... @15:25:29.90 SS.reconfigure :exhale @15:25:29.95 SS.hold @15:25:58.68 Intake.select :close @15:25:58.84 SSV.dial :exhaust @15:25:59.62 Exhaust.select :open @15:25:59.74 SS.seek 0ml @15:26:08.61 Exhaust.select :close @15:26:08.72 SSV.dial CBV @15:26:08.76 CBV.dial SSV @15:26:09.52 Allowing 130s for 25.0ml of remaining 650.0ml @15:26:09.53 Intake.select :open @15:26:11.70 Intake @15.6psia, vacuum from 10.0 to 13.0psi @15:26:11.72 SS.reconfigure :inhaling 0.59ml/s @15:26:11.77 SS.seek 25ml @15:26:47.28 Vacuum too high -- waiting up to 40 seconds @15:26:47.30 SS.reconfigure :exhale @15:26:47.35 SS.hold @15:27:01.91 Intake @16.0psia, vacuum from 10.0 to 13.0psi @15:27:01.93 SS.reconfigure :inhaling 0.47ml/s @15:27:01.98 SS.seek 25ml @15:27:14.62 Stroke completed -- waiting for pressure to equalize ... @15:27:14.63 SS.reconfigure :exhale @15:27:14.68 SS.hold @15:27:33.93 Intake.select :close @15:27:34.09 SSV.dial :exhaust @15:27:34.86 Exhaust.select :open @15:27:34.97 SS.seek 0ml @15:27:43.96 Exhaust.select :close @15:27:44.08 SSV.dial CBV @15:27:44.11 CBV.dial SSV @15:27:44.89 Allowing 130s for 25.0ml of remaining 625.0ml @15:27:44.90 Intake.select :open @15:27:47.07 Intake @15.6psia, vacuum from 10.0 to 13.0psi @15:27:47.09 SS.reconfigure :inhaling 0.47ml/s @15:27:47.14 SS.seek 25ml @15:28:42.26 Stroke completed -- waiting for pressure to equalize ... @15:28:42.27 SS.reconfigure :exhale @15:28:42.32 SS.hold @15:29:15.38 Intake.select :close @15:29:15.54 SSV.dial :exhaust @15:29:16.78 Exhaust.select :open @15:29:16.90 SS.seek 0ml @15:29:25.95 Exhaust.select :close @15:29:26.06 SSV.dial CBV @15:29:26.09 CBV.dial SSV @15:29:26.87 Allowing 130s for 25.0ml of remaining 600.0ml @15:29:26.88 Intake.select :open @15:29:29.05 Intake @15.6psia, vacuum from 10.0 to 13.0psi @15:29:29.07 SS.reconfigure :inhaling 0.47ml/s @15:29:29.12 SS.seek 25ml @15:30:12.69 Vacuum too high -- waiting up to 40 seconds @15:30:12.71 SS.reconfigure :exhale @15:30:12.76 SS.hold @15:30:31.33 Intake @16.3psia, vacuum from 10.0 to 13.0psi @15:30:31.35 SS.reconfigure :inhaling 0.38ml/s @15:30:31.40 SS.seek 25ml @15:30:46.11 Stroke completed -- waiting for pressure to equalize ... @15:30:46.13 SS.reconfigure :exhale @15:30:46.18 SS.hold @15:31:08.65 Intake.select :close @15:31:09.29 SSV.dial :exhaust @15:31:10.07 Exhaust.select :open @15:31:10.19 SS.seek 0ml @15:31:18.99 Exhaust.select :close @15:31:19.11 SSV.dial CBV @15:31:19.14 CBV.dial SSV @15:31:19.91 Allowing 130s for 25.0ml of remaining 575.0ml @15:31:19.93 Intake.select :open @15:31:22.09 Intake @15.6psia, vacuum from 10.0 to 13.0psi @15:31:22.11 SS.reconfigure :inhaling 0.38ml/s @15:31:22.16 SS.seek 25ml @15:32:30.85 Stroke completed -- waiting for pressure to equalize ... @15:32:30.86 SS.reconfigure :exhale @15:32:30.91 SS.hold @15:33:08.62 recoverPRVreagent08 @15:33:08.63 PTV.dial :mixing @15:33:08.64 PBV.dial :waste1 @15:33:09.00 delay 30 seconds @15:33:09.84 Intake.select :close @15:33:10.00 SSV.dial :exhaust @15:33:10.78 Exhaust.select :open @15:33:10.90 SS.seek 0ml @15:33:19.97 Exhaust.select :close @15:33:20.09 SSV.dial CBV @15:33:20.12 CBV.dial SSV @15:33:20.88 Allowing 130s for 25.0ml of remaining 550.0ml @15:33:20.89 Intake.select :open @15:33:23.06 Intake @15.6psia, vacuum from 10.0 to 13.0psi @15:33:23.08 SS.reconfigure :inhaling 0.38ml/s @15:33:23.13 SS.seek 25ml @15:33:39.02 PBV.dial between PSV and waste1 @15:33:39.39 PSV.dial :air @15:33:39.86 PS.reconfigure :fast @15:33:39.91 PS.seek 2.5ml @15:34:04.07 PSV.dial :mixloop @15:34:04.54 delay 2 seconds @15:34:06.56 PSV.dial :air @15:34:07.03 delay 2 seconds @15:34:09.04 PSV.dial :mixloop @15:34:09.51 delay 2 seconds @15:34:11.53 PSV.dial :air @15:34:12.00 PS.seek 0ml @15:34:26.12 Vacuum too high -- waiting up to 40 seconds @15:34:26.14 SS.reconfigure :exhale @15:34:26.19 SS.hold @15:34:35.36 washCycleDA #1 of 3 @15:34:35.37 PSR.select :air @15:34:35.38 PSV.dial PSR @15:34:36.73 Pulling 0.5ml of PSR.wash @15:34:36.74 PSR.select :wash @15:34:36.86 PS.reconfigure :default @15:34:36.91 PS.seek 0.5ml @15:34:42.82 delay 5 seconds @15:34:47.86 PSR.select :air @15:34:47.98 PS.seek 2ml @15:34:51.68 Intake @16.3psia, vacuum from 10.0 to 13.0psi @15:34:51.70 SS.reconfigure :inhaling 0.31ml/s @15:34:51.75 SS.seek 25ml @15:34:58.25 Stroke completed -- waiting for pressure to equalize ... @15:34:58.26 SS.reconfigure :exhale @15:34:58.31 SS.hold @15:35:04.16 PTV.dial :puck @15:35:04.93 PSV.dial PTV @15:35:05.68 PBV.dial :waste1 @15:35:06.10 PS.seek 1.5ml @15:35:12.10 delay 3 seconds @15:35:15.12 PBV.dial between PSV and waste1 @15:35:15.48 PS.seek 0ml @15:35:20.89 Intake.select :close @15:35:21.06 SSV.dial :exhaust @15:35:21.81 Exhaust.select :open @15:35:21.93 SS.seek 0ml @15:35:31.09 Exhaust.select :close @15:35:31.21 SSV.dial CBV @15:35:31.25 CBV.dial SSV @15:35:31.57 delay 2:00 @15:35:32.00 Allowing 130s for 25.0ml of remaining 525.0ml @15:35:32.02 Intake.select :open @15:35:34.18 Intake @15.6psia, vacuum from 10.0 to 13.0psi @15:35:34.20 SS.reconfigure :inhaling 0.31ml/s @15:35:34.25 SS.seek 25ml @15:37:00.97 Stroke completed -- waiting for pressure to equalize ... @15:37:00.98 SS.reconfigure :exhale @15:37:01.03 SS.hold @15:37:31.59 recoverPSRreagent08 @15:37:31.60 PTV.dial :puck @15:37:31.79 PBV.dial :waste1 @15:37:32.18 delay 30 seconds @15:37:41.21 SS.reconfigure :default @15:37:41.27 SS.hold @15:37:41.34 Intake.select :close @15:37:41.51 ShallowSampler::Clogged in WCR -- Intake @15psia & Outlet @13psia won't equalize after 500.0ml @15:37:41.52 SSV.dial :exhaust @15:37:42.28 Exhaust.select :open @15:37:42.40 SS.reconfigure :exhale @15:37:42.45 SS.seek 0ml @15:37:51.67 Exhaust.select :close @15:37:51.78 Sampled 500.0ml @15:37:51.80 Drying puck before fixing for 5:00 @15:37:51.81 CBV.dial :waste2b @15:37:52.57 delay 1:00 @15:38:02.20 PBV.dial between PSV and waste1 @15:38:02.57 PSR.select :air @15:38:02.59 PSV.dial PSR @15:38:03.35 PS.reconfigure :fast @15:38:03.40 PS.seek 2.5ml @15:38:27.25 PSV.dial PTV @15:38:28.49 delay 5 seconds @15:38:33.51 PSV.dial PSR @15:38:34.27 delay 5 seconds @15:38:39.29 PSV.dial PTV @15:38:40.05 delay 5 seconds @15:38:45.07 PSV.dial :air @15:38:45.84 PS.seek 0ml @15:38:52.59 CSV.dial :air @15:38:53.06 CS.reconfigure :fast @15:38:53.11 CS.seek 10ml @15:39:09.45 washCycleDA #2 of 3 @15:39:09.46 PSR.select :air @15:39:09.47 PSV.dial PSR @15:39:10.80 Pulling 0.5ml of PSR.wash @15:39:10.81 PSR.select :wash @15:39:10.93 PS.reconfigure :default @15:39:10.98 PS.seek 0.5ml @15:39:16.91 delay 5 seconds @15:39:17.92 Pumping wcr puck from tube 3 up to 35psi... @15:39:17.93 CBV.dial SSV @15:39:19.20 CBV.dial between CSV and waste2b @15:39:20.17 CSV.dial between air and CTV @15:39:20.50 CS.seek 7.161ml @15:39:21.93 PSR.select :air @15:39:22.05 PS.seek 2ml @15:39:27.85 CTV.dial CSV,avoiding: :air @15:39:27.89 CSV.dial CTV @15:39:28.70 CS.reconfigure :pressurize @15:39:28.75 CS.seek 0ml @15:39:38.19 PTV.dial :puck @15:39:38.27 PSV.dial PTV @15:39:39.14 PBV.dial :waste1 @15:39:39.57 PS.seek 1.5ml @15:39:43.26 CBV.dial :waste2b @15:39:43.59 CS.reconfigure :default @15:39:43.64 CS.seek 0ml @15:39:45.53 delay 3 seconds @15:39:48.55 PBV.dial between PSV and waste1 @15:39:48.82 delay 1:00 @15:39:48.93 PS.seek 0ml @15:40:05.10 delay 2:00 @15:40:48.84 CTV.dial :air,avoiding: :intake @15:40:50.18 Fix #1 of 2 (with RNAlater) for 2:30 @15:40:50.20 CSV.dial CSR @15:40:50.63 Pulling 2ml of CSR.RNAlater @15:40:50.64 CSR.select :RNAlater @15:40:50.76 CS.seek 2ml @15:40:57.39 delay 5 seconds @15:41:02.43 CSR.select :air @15:41:02.54 CS.reconfigure :fast @15:41:02.59 CS.seek 10ml @15:41:22.29 Pumping wcr puck from tube 3 up to 35psi... @15:41:22.31 CBV.dial SSV @15:41:23.56 CBV.dial between CSV and waste2b @15:41:24.52 CSV.dial between air and CTV @15:41:25.12 CS.seek 7.062ml @15:41:32.70 CTV.dial CSV,avoiding: :air @15:41:32.73 CSV.dial CTV @15:41:34.10 CS.reconfigure :pressurize @15:41:34.16 CS.seek 0ml @15:41:52.24 delay 2:30 @15:42:05.12 recoverPSRreagent08 @15:42:05.13 PTV.dial :puck @15:42:05.20 PBV.dial :waste1 @15:42:05.58 delay 30 seconds @15:42:35.61 PBV.dial between PSV and waste1 @15:42:35.98 PSR.select :air @15:42:36.00 PSV.dial PSR @15:42:36.76 PS.reconfigure :fast @15:42:36.80 PS.seek 2.5ml @15:43:00.72 PSV.dial PTV @15:43:01.49 delay 5 seconds @15:43:06.51 PSV.dial PSR @15:43:07.27 delay 5 seconds @15:43:12.29 PSV.dial PTV @15:43:13.05 delay 5 seconds @15:43:18.07 PSV.dial :air @15:43:18.83 PS.seek 0ml @15:43:42.31 washCycleDA #3 of 3 @15:43:42.32 PSR.select :air @15:43:42.34 PSV.dial PSR @15:43:43.67 Pulling 0.5ml of PSR.wash @15:43:43.68 PSR.select :wash @15:43:43.80 PS.reconfigure :default @15:43:43.85 PS.seek 0.5ml @15:43:49.84 delay 5 seconds @15:43:54.86 PSR.select :air @15:43:54.98 PS.seek 2ml @15:44:11.12 PTV.dial :puck @15:44:11.20 PSV.dial PTV @15:44:11.95 PBV.dial :waste1 @15:44:12.39 PS.seek 1.5ml @15:44:18.40 delay 3 seconds @15:44:21.42 PBV.dial between PSV and waste1 @15:44:21.80 PS.seek 0ml @15:44:22.26 CBV.dial :waste2b @15:44:22.60 CS.reconfigure :default @15:44:23.12 CS.seek 0ml @15:44:24.22 delay 1:00 @15:44:37.94 delay 2:00 @15:45:24.25 CTV.dial :air,avoiding: :intake @15:45:25.57 Fix #2 of 2 (with RNAlater) for 2:30 @15:45:25.59 CSV.dial CSR @15:45:26.03 Pulling 2ml of CSR.RNAlater @15:45:26.04 CSR.select :RNAlater @15:45:26.16 CS.seek 2ml @15:45:32.34 delay 5 seconds @15:45:37.36 CSR.select :air @15:45:37.49 CS.reconfigure :fast @15:45:37.54 CS.seek 10ml @15:45:57.38 Pumping wcr puck from tube 3 up to 35psi... @15:45:57.40 CBV.dial SSV @15:45:58.65 CBV.dial between CSV and waste2b @15:45:59.62 CSV.dial between air and CTV @15:46:00.23 CS.seek 7.078ml @15:46:07.79 CTV.dial CSV,avoiding: :air @15:46:07.82 CSV.dial CTV @15:46:09.19 CS.reconfigure :pressurize @15:46:09.25 CS.seek 0ml @15:46:27.12 delay 2:30 @15:46:37.96 recoverPSRreagent08 @15:46:37.97 PTV.dial :puck @15:46:38.04 PBV.dial :waste1 @15:46:38.42 delay 30 seconds @15:47:08.44 PBV.dial between PSV and waste1 @15:47:08.82 PSR.select :air @15:47:08.84 PSV.dial PSR @15:47:09.61 PS.reconfigure :fast @15:47:09.66 PS.seek 2.5ml @15:47:33.49 PSV.dial PTV @15:47:34.26 delay 5 seconds @15:47:39.28 PSV.dial PSR @15:47:40.04 delay 5 seconds @15:47:45.06 PSV.dial PTV @15:47:45.82 delay 5 seconds @15:47:50.84 PSV.dial :air @15:47:51.73 PS.seek 0ml @15:48:15.09 Gate.power :camera,:ON @15:48:15.10 Mixing DA Substrates @15:48:15.11 PSR.select :air @15:48:15.13 PSV.dial PSR @15:48:16.55 Pulling 0.25ml of PSR.sub1 @15:48:16.57 PSR.select :sub1 @15:48:16.78 PS.reconfigure :default @15:48:16.90 PS.seek 0.25ml @15:48:20.31 delay 5 seconds @15:48:25.33 Pulling 0.25ml of PSR.sub2 @15:48:25.35 PSR.select :sub2 @15:48:25.47 PS.seek 0.5ml @15:48:28.81 delay 5 seconds @15:48:33.83 PSR.select :air @15:48:33.94 PS.seek 2ml @15:48:50.62 PTV.dial :puck @15:48:50.70 PSV.dial PTV @15:48:51.48 PBV.dial :waste1 @15:48:51.92 PS.seek 1.5ml @15:48:57.14 CBV.dial :waste2b @15:48:57.49 CS.reconfigure :default @15:48:57.54 CS.seek 0ml @15:48:57.81 delay 3 seconds @15:48:59.00 delay 1:00 @15:49:00.82 PBV.dial between PSV and waste1 @15:49:01.20 PS.seek 0ml @15:49:17.40 delay 10 seconds @15:49:27.43 recoverPSRreagent08 @15:49:27.44 PTV.dial :puck @15:49:27.51 PBV.dial :waste1 @15:49:27.90 delay 30 seconds @15:49:57.93 PBV.dial between PSV and waste1 @15:49:58.30 PSR.select :air @15:49:58.32 PSV.dial PSR @15:49:59.02 CTV.dial :air,avoiding: :intake @15:49:59.11 PS.reconfigure :fast @15:49:59.16 PS.seek 2.5ml @15:50:23.52 PSV.dial PTV @15:50:24.31 delay 5 seconds @15:50:29.33 PSV.dial PSR @15:50:30.21 delay 5 seconds @15:50:35.23 PSV.dial PTV @15:50:35.99 delay 5 seconds @15:50:41.01 PSV.dial :air @15:50:41.79 PS.seek 0ml @15:51:05.77 Additional air clear @15:51:05.78 PSR.select :air @15:51:05.80 PSV.dial PSR @15:51:07.16 Pulling 2ml of PSR.air @15:51:07.17 PSR.select :air @15:51:07.20 PS.reconfigure :default @15:51:07.24 PS.seek 2ml @15:51:28.52 delay 5 seconds @15:51:33.54 PTV.dial :puck @15:51:33.63 PSV.dial PTV @15:51:34.40 PBV.dial :waste1 @15:51:34.83 PS.seek 1.5ml @15:51:40.80 delay 3 seconds @15:51:43.82 PBV.dial between PSV and waste1 @15:51:44.19 delay 1 second @15:51:45.21 PSV.dial :waste1 @15:51:46.29 PS.seek 0ml @15:52:02.51 Processing Puck Heater at 28C before imaging @15:52:02.52 PH.coast @15:52:02.63 Imaging da puck in Processing Clamp while storing it to tube 2 @15:52:02.81 SC.reconfigure :tight @15:52:02.86 SC.seek 2 @15:52:06.46 SC.configure :limp @15:52:07.17 Elbow.reconfigure :tight @15:52:07.22 Elbow.seek 2 @15:52:09.01 Forearm.reconfigure :tight @15:52:09.07 Forearm.seek 2 + 450 counts @15:52:10.09 SE.reconfigure :pick @15:52:10.15 SE.seek :beamBreak @15:52:12.03 SE.configure :default @15:52:12.20 Elbow.reconfigure :limp @15:52:12.25 Forearm.reconfigure :loose @15:52:12.30 Forearm.seek 2 @15:52:12.52 Elbow.hold @15:52:12.58 Elbow.reconfigure :tight @15:52:12.69 SE.seek 20.11 Pucks below Top Plate @15:52:13.22 Forearm.force -20 @15:52:13.27 Forearm.seek 2 + 306 counts @15:52:13.67 Forearm.reconfigure :tight @15:52:13.72 Forearm.seek 2 + 450 counts @15:52:14.00 SE.seek 21.11 Pucks below Top Plate @15:52:14.66 Forearm.reconfigure :loose @15:52:14.71 Forearm.seek :retract @15:52:15.67 Elbow.seek PC @15:52:18.16 Elbow.reconfigure :loose @15:52:18.26 Forearm.seek PC @15:52:19.26 Hand.close @15:52:19.91 PC.seek :open @15:52:24.73 Forearm.seek :retract @15:52:25.74 Elbow.reconfigure :tight @15:52:25.79 Elbow.seek 2 @15:52:28.40 Forearm.reconfigure :tight @15:52:28.45 Forearm.seek 2 + 450 counts @15:52:29.45 Hand.open @15:52:30.59 SE.seek 20.76 Pucks below Top Plate @15:52:30.86 SE.reconfigure :seal @15:52:30.91 SE.seek 19.79 Pucks below Top Plate @15:52:31.43 Lights, camera, ACTION! @15:52:31.47 PuckCamera: snap -bin=2,2 -auto /var/log/esp/da23oct3115h575ml.tif @15:52:50.97 Waiting >20 seconds for da to relinquish FlushPuck @15:52:55.87 SX HX9/16 (USB:14): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 21.11 seconds /var/log/esp/da23oct3115h575ml.tif: TIFF Upload Complete @15:52:55.91 PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da23oct3115h575ml15s.tif @15:53:11.59 SX HX9/16 (USB:14): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15 seconds /var/log/esp/midres/da23oct3115h575ml15s.tif: TIFF Upload Complete @15:53:11.62 PuckCamera: snap -bin=2,2 20.0 /var/log/esp/midres/da23oct3115h575ml20s.tif @15:53:32.27 SX HX9/16 (USB:14): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 20 seconds /var/log/esp/midres/da23oct3115h575ml20s.tif: TIFF Upload Complete @15:53:32.29 Gate.power :camera,:OFF @15:53:32.31 DAfans.email "21.11 sec auto-exposure",Subject: "da23oct3115h575ml images!" @15:53:32.78 Elbow.seek 2 @15:53:33.02 Forearm.seek 2 + 450 counts @15:53:33.25 SE.reconfigure :gently @15:53:33.31 SE.seek 20.51 Pucks below Top Plate @15:53:34.07 Elbow.reconfigure :limp @15:53:34.13 Forearm.reconfigure :loose @15:53:34.20 Forearm.seek 2 @15:53:34.48 Elbow.hold @15:53:34.56 Elbow.reconfigure :tight @15:53:34.62 SE.reconfigure :default @15:53:34.71 SE.seek 20.69 Pucks below Top Plate @15:53:35.12 Forearm.force -20 @15:53:35.18 Forearm.seek 2 + 311 counts @15:53:35.70 Forearm.reconfigure :tight @15:53:35.82 Forearm.seek 2 + 450 counts @15:53:36.23 SE.seek :down @15:53:37.50 Forearm.reconfigure :loose @15:53:37.65 Forearm.seek :retract @15:53:37.71 SE.stop @15:53:38.89 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @15:53:40.40 Elbow.seek FlushPuck::Garage @15:53:42.40 Elbow.reconfigure :loose @15:53:42.61 Forearm.seek :garage @15:53:43.66 Hand.close @15:53:44.63 Forearm.seek :retract @15:53:45.69 Elbow.reconfigure :tight @15:53:45.81 Elbow.seek PC @15:53:47.22 Elbow.reconfigure :loose @15:53:47.43 Forearm.reconfigure :tight @15:53:47.53 Forearm.seek PC @15:53:49.11 PC.seek :closed @15:53:53.95 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.62 @15:53:54.11 Hand.open @15:53:54.91 Forearm.reconfigure :loose @15:53:55.00 Forearm.seek :retract @15:53:56.03 PSR.select :air @15:53:56.09 Storing wcr puck in Collection Clamp to tube 2 @15:53:56.13 PSV.dial PSR @15:53:56.61 SC.reconfigure :tight @15:53:56.71 SC.seek 2 @15:53:56.85 Pulling 0.5ml of PSR.flush @15:53:56.91 PSR.select :flush @15:53:57.07 SC.configure :limp @15:53:57.47 PS.seek 0.5ml @15:53:57.73 Elbow.reconfigure :tight @15:53:57.84 Elbow.seek 2 @15:54:01.64 Forearm.reconfigure :tight @15:54:01.75 Forearm.seek 2 + 450 counts @15:54:02.90 SE.reconfigure :pick @15:54:03.02 SE.seek :beamBreak @15:54:03.54 delay 5 seconds @15:54:04.98 SE.configure :default @15:54:05.32 Elbow.reconfigure :limp @15:54:05.44 Forearm.reconfigure :loose @15:54:05.52 Forearm.seek 2 @15:54:05.80 Elbow.hold @15:54:05.94 Elbow.reconfigure :tight @15:54:06.14 SE.seek 21.1 Pucks below Top Plate @15:54:06.87 Forearm.force -20 @15:54:06.97 Forearm.seek 2 + 320 counts @15:54:07.46 Forearm.reconfigure :tight @15:54:07.56 Forearm.seek 2 + 450 counts @15:54:07.84 SE.seek 22.1 Pucks below Top Plate @15:54:08.69 PSR.select :air @15:54:08.86 Forearm.reconfigure :loose @15:54:08.98 Forearm.seek :retract @15:54:09.09 PS.seek 2.5ml @15:54:09.91 Elbow.seek CC @15:54:10.24 upsync @15:54:11.56 Elbow.reconfigure :loose @15:54:11.66 Forearm.seek CC @15:54:12.61 Hand.close @15:54:13.28 CC.seek :open @15:54:18.43 Forearm.seek :retract @15:54:19.52 Elbow.reconfigure :tight @15:54:19.57 Elbow.seek 2 @15:54:21.35 Elbow.reconfigure :limp @15:54:21.41 Forearm.seek 2 @15:54:22.39 Elbow.hold @15:54:22.46 Elbow.reconfigure :tight @15:54:22.51 Hand.open @15:54:23.28 Forearm.force -20 @15:54:23.33 Forearm.seek 2 + 303 counts @15:54:23.65 Forearm.reconfigure :tight @15:54:23.70 Forearm.seek 2 + 450 counts @15:54:23.99 SE.seek :down @15:54:25.13 Forearm.reconfigure :loose @15:54:25.20 Forearm.seek :retract @15:54:25.22 SE.stop @15:54:26.25
Starting finalPurge @15:54:30.39 delay 1 second @15:54:31.41 PRV.dial :waste2,avoiding: :ab2 @15:54:33.27 PTV.dial :PRVmixing @15:54:34.58 PSV.dial :mixloop @15:54:35.62 PS.seek :empty @15:54:45.42
Waiting >20 seconds for psFlush to relinquish FlushPuck @15:55:02.05 PSR.select :air @15:55:02.07 PSV.dial PSR @15:55:03.11 Pulling 0.5ml of PSR.flush @15:55:03.12 PSR.select :flush @15:55:03.24 PS.seek 0.5ml @15:55:09.14 delay 5 seconds @15:55:14.16 PSR.select :air @15:55:14.28 PS.seek 2.5ml @15:55:35.61 delay 1 second @15:55:36.63 PBV.dial :waste1 @15:55:37.00 PTV.dial :mixing @15:55:37.77 PSV.dial :mixloop @15:55:38.82 PS.seek :empty @15:56:05.14 PSV.dial PSR @15:56:06.19 PSR.select :flush @15:56:06.75 PS.seek 1ml @15:56:17.83 PSR.select :air @15:56:17.95 PS.seek 2.5ml @15:56:34.11 PSV.dial PTV @15:56:34.99 PTV.dial :puck @15:56:35.72 PBV.dial :waste1 @15:56:35.80 PS.seek 0ml @15:57:02.11 PSV.dial PSR @15:57:02.84 PSR.select :flush @15:57:02.97 PS.seek 0.5ml @15:57:09.01 PSR.select :air @15:57:09.13 PS.seek 2.5ml @15:57:30.32 PSV.dial PTV @15:57:31.20 PBV.dial PSV @15:57:31.77 PS.seek 0ml @15:57:58.32 PSV.dial PBV @15:57:58.78 PS.seek 2.5ml @15:58:25.17 PSV.dial :waste1 @15:58:25.91 PS.seek 0ml @15:58:52.29 PSV.dial PSR @15:58:52.76 PSR.select :air @15:58:52.79 PS.seek 2.5ml @15:59:19.17 PSV.dial PTV @15:59:19.90 PBV.dial PSV @15:59:20.08 PS.seek 0ml @15:59:46.38 PSV.dial PBV @15:59:46.86 PS.seek 2.5ml @16:00:13.17 PSV.dial :waste1 @16:00:13.91 PS.seek 0ml @16:00:40.23 PSV.dial PSR @16:00:40.70 PSR.select :flush @16:00:40.82 PS.seek 1ml @16:00:51.82 PSR.select :air @16:00:51.94 PS.seek 2.5ml @16:01:08.04 PSV.dial PTV @16:01:08.78 PTV.dial :puck @16:01:08.85 PBV.dial :waste1 @16:01:09.41 PS.seek 0ml @16:01:35.76 PSV.dial PSR @16:01:36.63 PSR.select :flush @16:01:36.76 PS.seek 0.5ml @16:01:42.73 PSR.select :air @16:01:42.85 PS.seek 2.5ml @16:02:04.60 PSV.dial PTV @16:02:05.34 PBV.dial PSV @16:02:05.89 PS.seek 0ml @16:02:32.32 PSV.dial PBV @16:02:32.80 PS.seek 2.5ml @16:02:59.16 PSV.dial :waste1 @16:02:59.90 PS.seek 0ml @16:03:26.22 PSV.dial PSR @16:03:26.69 PSR.select :air @16:03:26.72 PS.seek 2.5ml @16:03:53.10 PSV.dial PTV @16:03:53.83 PBV.dial PSV @16:03:53.92 PS.seek 0ml @16:04:20.35 PSV.dial PBV @16:04:20.82 PS.seek 2.5ml @16:04:47.10 PSV.dial :waste1 @16:04:47.84 PS.seek 0ml @16:05:14.23
Loading FlushPuck into Collection Clamp from Processing Clamp @16:05:14.41 Elbow.seek PC @16:05:16.90 Elbow.reconfigure :loose @16:05:17.01 Forearm.seek PC @16:05:17.96 Hand.close @16:05:18.52 PC.seek :open @16:05:23.25 Forearm.seek :retract @16:05:24.27 Elbow.reconfigure :tight @16:05:24.32 Elbow.seek CC @16:05:27.93 Elbow.reconfigure :loose @16:05:28.04 Forearm.reconfigure :tight @16:05:28.08 Forearm.seek CC @16:05:29.28 CC.seek :closed @16:05:33.80 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.38 @16:05:33.87 Hand.open @16:05:34.59 Forearm.reconfigure :loose @16:05:34.63 Forearm.seek :retract @16:05:35.60 Flushing the Collection Valve::Manifold @16:05:35.73 CSV.dial CSR @16:05:36.17 Pulling 5ml of CSR.flush @16:05:36.18 CSR.select :flush @16:05:36.29 CS.seek 5ml @16:05:49.89 delay 5 seconds @16:05:54.90 CSR.select :air @16:05:55.11 CS.reconfigure :fast @16:05:55.16 CS.seek 7.5ml @16:06:01.92 CSV.dial :waste @16:06:03.20 CS.reconfigure :default @16:06:03.25 CS.seek 0ml @16:06:23.26 Flushing lower manifold's waste1 port @16:06:23.38 CSV.dial :flush @16:06:23.84 CS.reconfigure :fast @16:06:23.89 CS.seek 5ml @16:06:36.51 delay 5 seconds @16:06:41.53 CSV.dial :air @16:06:42.04 CS.seek 7.001ml @16:06:47.54 CSV.dial CTV @16:06:47.57
CTV.dial CSV @16:06:48.98 CBV.dial :waste1 @16:06:49.75 CS.seek 0ml @16:07:06.45 Flushing lower manifold's waste2 port @16:07:06.57 CSV.dial :flush @16:07:07.30 CS.seek 5ml @16:07:19.92 delay 5 seconds @16:07:24.93 CSV.dial :air @16:07:25.46 CS.seek 7ml @16:07:30.95 CSV.dial CTV @16:07:30.98
CTV.dial CSV @16:07:31.48 CBV.dial :waste2 @16:07:32.53 CS.seek 0ml @16:07:49.14 Flushing lower manifold's waste2b port @16:07:49.26 CSV.dial :flush @16:07:50.00 CS.seek 5ml @16:08:02.82 delay 5 seconds @16:08:07.84 CSV.dial :air @16:08:08.81 CS.seek 7ml @16:08:14.29 CSV.dial CTV @16:08:14.32
CTV.dial CSV @16:08:14.76 CBV.dial :waste2b @16:08:15.81 CS.seek 0ml @16:08:32.29 Disinfecting external Intake with kill @16:08:32.92 CSV.dial CSR @16:08:33.35 Pulling 8ml of CSR.kill @16:08:33.36 CSR.select :kill @16:08:33.48 CS.seek 8ml @16:08:53.29 delay 5 seconds @16:08:58.31 CSR.select :air @16:08:58.43 CS.seek 10ml @16:09:03.92 CSV.dial CBV @16:09:03.95
CBV.dial CSV @16:09:05.02 CTV.dial :intake @16:09:05.76 Intake.select :open @16:09:05.87 CS.seek 2ml @16:09:24.63 Intake.select :close @16:09:24.73 CTV.dial :air @16:09:25.50 CS.reconfigure :default @16:09:25.54 CS.seek 0ml @16:09:31.54 CSV.dial CSR @16:09:32.55 Pulling 5ml of CSR.flush @16:09:32.56 CSR.select :flush @16:09:32.68 CS.seek 5ml @16:09:46.41 delay 5 seconds @16:09:51.43 CSR.select :air @16:09:51.55 CS.seek 10ml @16:10:05.38 CSV.dial CBV @16:10:06.40 CS.seek 0ml @16:10:32.79 Garaging FlushPuck in Collection Clamp @16:10:32.86 Elbow.reconfigure :tight @16:10:32.91 Elbow.seek CC @16:10:33.13 Elbow.reconfigure :loose @16:10:33.24 Forearm.seek CC @16:10:34.27 Hand.close @16:10:34.90 CC.seek :open @16:10:39.56 Forearm.seek :retract @16:10:40.60 Elbow.reconfigure :tight @16:10:40.65 Elbow.seek :garage @16:10:43.52 Elbow.reconfigure :loose @16:10:43.63 Forearm.reconfigure :tight @16:10:43.67 Forearm.seek :garage @16:10:44.63 Hand.open @16:10:45.35 Forearm.reconfigure :loose @16:10:45.40 Forearm.seek :retract @16:10:46.43 Completed [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] begun 13:51:45 @16:10:46.48 Asleep after completing DA_WCR_103123 assays @16:10:46.50 GoodNews.email "{1 => 33,\n 2 => 23,\n 3 => 10,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@16:00:55, 25% flow, 19.1C, 23% humidity, 14.6psia, 13.730V, 0.369A, 254.688Ah, 5.06W\nSampled 500.0ml",Subject: "Asleep after completing DA_WCR_103123 assays" @16:10:50.61 upsync + ESPwhich DA_WCR_110223.rb + fn=DA_WCR_110223.rb + IFS=: + set /home/esp/esp2/mission . + fullPath=/home/esp/esp2/mission/DA_WCR_110223.rb + [ -f /home/esp/esp2/mission/DA_WCR_110223.rb ] + realpath /home/esp/esp2/mission/DA_WCR_110223.rb + return 0 + scriptPath=/home/esp/esp2local/mission/DA_WCR_110223.rb + exec ruby /home/esp/esp2/lib/esp.rb /home/esp/esp2local/mission/DA_WCR_110223.rb @14:25:31.11UTC02-Nov-23
ESP2 v3.42 I2C Gateway v3.20 for core bus @14:25:33.75 Configuration for short puck jake 1/11/21 by brent@mbari.org /home/esp/esp2/type/shallow/jake/configure.rb @14:25:37.36 Polling Can every 10:00 @14:25:37.41 Loading /home/esp/esp2local/mission/DA_WCR_110223.rb mission @14:25:37.47 GoodNews.email "Starting DA_WCR_110223 w/pucks in tube 3\n{}\nCTD data unavailable\nCan@14:25:37, 28% flow, 16.9C, 23% humidity, 14.5psia, 13.744V, 0.272A, 265.966Ah, 3.74W",Subject: "DA_WCR_110223 begun" @14:25:37.50
Preparing to sleep until + 00:00:05 @14:25:37.54 Asleep til + 00:00:05 @14:25:37.56 Continuing at 14:25 @14:25:37.85 Gate.power :core,:OFF @14:25:37.86 Gate.power :raw,:OFF @14:25:37.87 Shutdown @14:25:40.53 GoodNews.email "Then, in tube 3: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_110223.rb:14\n{1 => 33,\n 2 => 23,\n 3 => 10,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@14:25:37, 28% flow, 16.8C, 23% humidity, 14.5psia, 13.739V, 0.272A, 265.966Ah, 3.74W",Subject: "Asleep until 14:25" @14:25:42.12 upsync @14:25:42.47
Powering core bus @14:25:45.01 upsync @14:25:48.27
Gate.power :raw,:ON @14:25:49.33 CS.configure :default @14:25:49.38 PS.configure :default @14:25:49.43 SS.configure :default @14:25:49.47 CC.configure :default @14:25:49.52 PC.configure :default @14:25:49.59 Forearm.configure :loose @14:25:49.64 Elbow.configure :loose @14:25:49.70 SC.configure :loose @14:25:49.75 SE.configure :default @14:25:49.80 CH.configure :default @14:25:49.83 PH.configure :default @14:25:49.88 All dwarf controllers running firmware version 3.85 @14:25:49.98 Valve.reset @14:25:50.25 Intake.select :close @14:25:50.29 Exhaust.select :close @14:25:50.30 CTV.dial :air @14:25:50.38 PSV.dial :air @14:25:50.43 CSV.dial :waste1 @14:25:50.56
Forearm.home.seek :retract @14:25:50.94 SSV.dial :spit,avoiding: :exhaust @14:25:51.33 Hand.close @14:25:51.37
Elbow.reconfigure :loose @14:25:51.47 Elbow.jog 1500 @14:25:51.64 PS.home.seek 0ml @14:25:51.82 CS.reconfigure :fast @14:25:51.87 SS.home.seek 0ml @14:25:51.92 CS.home.seek 0ml @14:25:52.06
Elbow.reconfigure :loose @14:25:52.10 Elbow.home.seek :clear @14:25:56.63 SE.home.seek :down @14:25:57.26 SC.reconfigure :tight @14:25:57.31 SC.home.seek :initial @14:25:59.90 PS.stop @14:26:25.62
SC.configure :limp @14:26:25.67 Forearm.seek :retract @14:26:25.74 Elbow.seek :hammer @14:26:26.77 Forearm.reconfigure :tight @14:26:26.82 Forearm.seek retracted @14:26:27.19 CC.home.seek :open @14:26:27.22 PC.home.seek :open @14:26:31.03
Phase [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] @14:26:31.04 Notice.email "At 14:26:30 began in tube 3: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_110223.rb:14\n{1 => 33,\n 2 => 23,\n 3 => 10,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@14:26:31, 28% flow, 16.7C, 23% humidity, 14.5psia, 13.734V, 0.290A, 265.972Ah, 3.99W",Subject: "Started DA_WCR_110223 phase initialPurge; da [1000, 1000] #da and wsr are run simultanelously" @14:26:31.06
Starting initialPurge @14:26:31.08 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:26:31.15 Hand.open @14:26:31.92 Elbow.reconfigure :tight @14:26:31.97 Elbow.seek FlushPuck::Garage @14:26:33.94 Elbow.reconfigure :loose @14:26:34.16 Forearm.reconfigure :loose @14:26:34.26 Forearm.seek :garage @14:26:35.25 Hand.close @14:26:35.63 upsync @14:26:36.04
Forearm.seek :retract @14:26:37.05 Elbow.reconfigure :tight @14:26:37.10 Elbow.seek CC @14:26:40.10 Elbow.reconfigure :loose @14:26:40.21 Forearm.reconfigure :tight @14:26:40.26 Forearm.seek CC @14:26:41.51 CC.seek :closed @14:26:46.05 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 1.03 @14:26:46.07 Puck::Warning in MAIN -- Retaining claim on Arm because Hand is likely holding a puck! @14:26:46.08 Slide::Error in MAIN -- No Puck Clamped in Collection Clamp @14:26:46.10 Waiting to Resume from Checkpoint "No Puck Clamped in Collection Clamp" @14:26:46.11 BadNews.email "No Puck Clamped in Collection Clamp",Subject: "Slide::Error in MAIN" @14:26:50.34 upsync @14:29:09.19 Session Begun @14:29:18.21 -> Flush FlushPuck is in Hand @14:29:37.21 -> Flush.garage @14:29:48.26 Waiting >20 seconds for MAIN to relinquish FlushPuck @14:29:55.86 UserAbort in dave -- User Abort!! @14:30:01.76 UserAbort in dave -- User Abort!! @14:31:21.43 UserAbort in dave -- User Abort!! @14:31:27.30 -> Hand Hand is closed on FlushPuck @14:31:30.27 -> Flush FlushPuck is in Hand @14:31:32.67 -> CC Collection Clamp closed @14:31:34.89 -> PC Processing Clamp open @14:31:53.02 -> Flush.PC @14:32:05.01 Waiting >20 seconds for MAIN to relinquish FlushPuck @14:32:08.58 UserAbort in dave -- User Abort!! @14:32:30.96 UserAbort in dave -- User Abort!! @14:32:58.53 UserAbort in dave -- User Abort!! @14:34:34.16 UserAbort in dave -- User Abort!! @14:35:26.37 UserAbort in dave -- User Abort!! @14:35:45.74 -> abort! 'missio failed' @14:35:45.76 CmdServer::Client::Error in dave -- Aborting Mission: missio failed @14:35:45.80 BadNews.email "missio failed",Subject: "DA_WCR_110223 ABORTED!" # @14:35:50.28 upsync @14:35:50.33 Storage::Warning in MAIN -- Exiting with FlushPuck in Hand @14:35:50.38 Session Ended + ESPwhich DA_WCR_110223.rb + fn=DA_WCR_110223.rb + IFS=: + set /home/esp/esp2/mission . + fullPath=/home/esp/esp2/mission/DA_WCR_110223.rb + [ -f /home/esp/esp2/mission/DA_WCR_110223.rb ] + realpath /home/esp/esp2/mission/DA_WCR_110223.rb + return 0 + scriptPath=/home/esp/esp2local/mission/DA_WCR_110223.rb + exec ruby /home/esp/esp2/lib/esp.rb /home/esp/esp2local/mission/DA_WCR_110223.rb @20:27:45.91UTC13-Nov-23
ESP2 v3.42 I2C Gateway v3.20 for core bus @20:27:48.59 Configuration for short puck jake 1/11/21 by brent@mbari.org /home/esp/esp2/type/shallow/jake/configure.rb @20:27:52.32 Polling Can every 10:00 @20:27:52.36 Cleanly exited at 20:27:29UTC13-Nov-23 @20:27:52.40 Loading /home/esp/esp2local/mission/DA_WCR_110223.rb mission @20:27:52.46 Preparing to sleep until + 00:00:05 @20:27:52.48 GoodNews.email "Starting DA_WCR_110223 w/pucks in tube 3\n{1 => 33,\n 2 => 23,\n 3 => 10,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@20:27:52, 33% flow, 14.2C, 28% humidity, 15.1psia, 13.749V, 0.272A, 0.059Ah, 3.74W",Subject: "DA_WCR_110223 begun" @20:27:52.54
Asleep til + 00:00:05 @20:27:52.55 Continuing at 20:27 @20:27:52.82 Gate.power :core,:OFF @20:27:52.83 Gate.power :raw,:OFF @20:27:52.84 Shutdown @20:27:55.64 GoodNews.email "Then, in tube 3: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_110223.rb:14\n{1 => 33,\n 2 => 23,\n 3 => 10,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@20:27:52, 33% flow, 14.2C, 28% humidity, 15.1psia, 13.749V, 0.266A, 0.059Ah, 3.66W",Subject: "Asleep until 20:27" @20:27:57.30 upsync @20:27:57.44
Powering core bus @20:28:00.13 upsync @20:28:03.08
Gate.power :raw,:ON @20:28:04.08 CS.configure :default @20:28:04.13 PS.configure :default @20:28:04.18 SS.configure :default @20:28:04.22 CC.configure :default @20:28:04.27 PC.configure :default @20:28:04.34 Forearm.configure :loose @20:28:04.39 Elbow.configure :loose @20:28:04.46 SC.configure :loose @20:28:04.50 SE.configure :default @20:28:04.55 CH.configure :default @20:28:04.58 PH.configure :default @20:28:04.62 All dwarf controllers running firmware version 3.85 @20:28:04.75 Valve.reset @20:28:05.01 Intake.select :close @20:28:05.04 Exhaust.select :close @20:28:05.07 CTV.dial :air @20:28:05.17 PSV.dial :air @20:28:05.29 CSV.dial :waste1 @20:28:05.32
Forearm.home.seek :retract @20:28:05.33 PS.home.seek 0ml @20:28:05.80 SSV.dial :spit,avoiding: :exhaust @20:28:05.98 CS.reconfigure :fast @20:28:06.01 SS.home.seek 0ml @20:28:06.11 CS.home.seek 0ml @20:28:06.17 Hand.close @20:28:06.21
Elbow.reconfigure :loose @20:28:06.30 Elbow.jog 1500 @20:28:06.81 Elbow.reconfigure :loose @20:28:06.85 Elbow.home.seek :clear @20:28:11.61 SE.home.seek :down @20:28:12.21 SC.reconfigure :tight @20:28:12.26 SC.home.seek :initial @20:28:13.59 PS.stop @20:28:40.58
SC.configure :limp @20:28:40.63 Forearm.seek :retract @20:28:40.70 Elbow.seek :hammer @20:28:41.74 Forearm.reconfigure :tight @20:28:41.78 Forearm.seek retracted @20:28:42.05 PC.home.seek :open @20:28:42.06
CC.home.seek :open @20:28:45.79 Phase [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] @20:28:45.80 Notice.email "At 20:28:45 began in tube 3: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_110223.rb:14\n{1 => 33,\n 2 => 23,\n 3 => 10,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@20:28:45, 34% flow, 14.2C, 27% humidity, 15.1psia, 13.676V, 0.302A, 0.064Ah, 4.14W",Subject: "Started DA_WCR_110223 phase initialPurge; da [1000, 1000] #da and wsr are run simultanelously" @20:28:45.83
Starting initialPurge @20:28:45.85 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @20:28:45.92 Hand.open @20:28:46.65 Elbow.reconfigure :tight @20:28:46.70 Elbow.seek FlushPuck::Garage @20:28:48.71 Elbow.reconfigure :loose @20:28:48.93 Forearm.reconfigure :loose @20:28:49.03 Forearm.seek :garage @20:28:49.99 Hand.close @20:28:50.24 upsync @20:28:50.76
Forearm.seek :retract @20:28:51.73 Elbow.reconfigure :tight @20:28:51.78 Elbow.seek CC @20:28:54.81 Elbow.reconfigure :loose @20:28:54.92 Forearm.reconfigure :tight @20:28:54.97 Forearm.seek CC @20:28:56.22 CC.seek :closed @20:29:00.81 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.47 @20:29:00.88 Hand.open @20:29:01.58 Forearm.reconfigure :loose @20:29:01.62 Forearm.seek :retract @20:29:02.63 Flushing the Collection Valve::Manifold @20:29:02.76 CSV.dial CSR @20:29:04.05 Pulling 5ml of CSR.flush @20:29:04.06 CSR.select :flush @20:29:04.17 CS.reconfigure :default @20:29:04.63 CS.seek 5ml @20:29:18.43 delay 5 seconds @20:29:23.45 CSR.select :air @20:29:23.61 CS.reconfigure :fast @20:29:23.66 CS.seek 7.5ml @20:29:30.42 CSV.dial :waste @20:29:31.73 CS.reconfigure :default @20:29:31.78 CS.seek 0ml @20:29:51.71 Purging external Intake @20:29:51.83 CSV.dial :flush @20:29:52.29 CS.reconfigure :fast @20:29:52.34 CS.seek 10ml @20:30:16.83 delay 5 seconds @20:30:21.86 CSV.dial CBV @20:30:21.88
CBV.dial CSV @20:30:22.64 CTV.dial :intake @20:30:23.44 Intake.select :open @20:30:23.55 CS.seek 0ml @20:30:47.20 Intake.select :close @20:30:47.31 CTV.dial :air @20:30:48.10 Flushing upper manifold @20:30:48.21 CSV.dial :flush @20:30:49.07 CS.seek 5ml @20:31:02.08 delay 5 seconds @20:31:07.10 CSV.dial :air @20:31:07.62 CS.seek 7ml @20:31:13.24 CSV.dial CBV @20:31:13.26
CBV.dial CSV @20:31:14.31 CTV.dial :air @20:31:14.40 CS.seek 0ml @20:31:31.36 delay 7 seconds @20:31:38.39 CS.seek 3ml @20:31:46.42 CSV.dial :waste @20:31:46.88 CS.seek 0ml @20:31:54.84 CSV.dial :flush @20:31:55.31 CS.seek 3ml @20:32:03.36 delay 5 seconds @20:32:08.38 CSV.dial :air @20:32:08.88 CS.seek 5ml @20:32:14.45 CSV.dial :air @20:32:14.54 CS.seek 0ml @20:32:27.08 delay 7 seconds @20:32:34.11 CS.seek 3ml @20:32:42.11 CSV.dial :waste @20:32:42.85 CS.seek 0ml @20:32:50.74 Garaging FlushPuck in Collection Clamp @20:32:50.81 Elbow.reconfigure :tight @20:32:50.86 Elbow.seek CC @20:32:51.19 Elbow.reconfigure :loose @20:32:51.29 Forearm.seek CC @20:32:52.28 Hand.close @20:32:52.88 CC.seek :open @20:32:57.92 Forearm.seek :retract @20:32:58.88 Elbow.reconfigure :tight @20:32:58.93 Elbow.seek :garage @20:33:01.91 Elbow.reconfigure :loose @20:33:02.00 Forearm.reconfigure :tight @20:33:02.05 Forearm.seek :garage @20:33:03.10 Hand.open @20:33:03.81 Forearm.reconfigure :loose @20:33:03.86 Forearm.seek :retract @20:33:04.84 da sampling at most 1000ml, wcr at most 1000ml @20:33:04.88 Loading sh1 puck from tube 3 into Collection Clamp @20:33:04.89 DAfans.email "Started da in tube 3 at 20:33:04UTC13-Nov-23\n{1 => 33,\n 2 => 23,\n 3 => 10,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@20:28:45, 34% flow, 14.2C, 27% humidity, 15.1psia, 13.676V, 0.302A, 0.064Ah, 4.14W",Subject: "da sampling at most 1000ml, wcr at most 1000ml" @20:33:05.15
SC.reconfigure :tight @20:33:05.22 SC.seek 3 @20:33:08.81 SC.configure :limp @20:33:08.92 upsync @20:33:09.08
Elbow.reconfigure :tight @20:33:09.13 Elbow.seek 3 @20:33:11.12 Forearm.reconfigure :tight @20:33:11.17 Forearm.seek 3 + 450 counts @20:33:12.18 SE.reconfigure :pick @20:33:12.24 SE.seek :beamBreak @20:33:14.77 SE.configure :default @20:33:14.93 Elbow.seek 3 @20:33:15.12 Forearm.seek 3 + 300 counts @20:33:15.38 Forearm.reconfigure :loose @20:33:15.43 Hand.close @20:33:16.02 SE.seek :down @20:33:16.59
Forearm.seek :retract @20:33:17.58 Elbow.seek CC @20:33:18.21 SE.stop @20:33:19.26
Elbow.reconfigure :loose @20:33:19.37 Forearm.reconfigure :tight @20:33:19.42 Forearm.seek CC @20:33:20.75 CC.seek :closed @20:33:25.27 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.29 @20:33:25.29 Hand.open @20:33:25.94 Forearm.reconfigure :loose @20:33:25.99 Forearm.seek :retract @20:33:26.96 collectSample 1000ml w/bubble point of 24psi @20:33:26.97 Priming 7ml @20:33:26.98 Intake.select :close @20:33:26.99 Exhaust.select :close @20:33:27.00 SS.hold @20:33:27.22 Calibrating pressure and temperature sensors @20:33:27.25 CTV.dial :air @20:33:27.31 SSV.dial :spit,avoiding: :exhaust @20:33:27.39 SS.reconfigure :fast @20:33:27.44 SS.seek 5ml @20:33:30.37 SSV.dial CBV @20:33:30.39
CBV.dial SSV @20:33:31.76 delay 3.8 seconds @20:33:35.69 Sensor limits: Intake max 107.9psia; Outlet max 98.5psia @20:33:35.70 SSV.dial :spit @20:33:37.05 SS.seek 0ml @20:33:39.60 SSV.dial CBV @20:33:40.92 CTV.dial :intake @20:33:41.72 SSV.dial CBV @20:33:41.75
CBV.dial SSV @20:33:41.88 Allowing 40s for 7.0ml of remaining 7.0ml @20:33:41.89 Intake.select :open @20:33:44.05 Intake @14.8psia, vacuum from 9.3 to 12.3psi @20:33:44.07 SS.reconfigure :inhaling 2.20ml/s @20:33:44.12 SS.seek 7ml @20:33:50.45 Stroke completed -- waiting for pressure to equalize ... @20:33:50.46 SS.reconfigure :exhale @20:33:50.50 SS.hold @20:33:57.21 Intake.select :close @20:33:57.36 SSV.dial :exhaust @20:33:58.12 Exhaust.select :open @20:33:58.23 SS.seek 0ml @20:34:02.48 Exhaust.select :close @20:34:02.59 Sampling 1000.0ml @20:34:02.60 Intake.select :close @20:34:02.61 Exhaust.select :close @20:34:02.63 SS.reconfigure :default @20:34:02.67 SS.hold @20:34:02.88 CTV.dial :intake @20:34:03.11 SSV.dial CBV @20:34:03.14
CBV.dial SSV @20:34:03.95 Allowing 130s for 25.0ml of remaining 1000.0ml @20:34:03.96 Intake.select :open @20:34:06.13 Intake @15.0psia, vacuum from 9.5 to 12.5psi @20:34:06.14 SS.reconfigure :inhaling 2.20ml/s @20:34:06.19 SS.seek 25ml @20:34:12.42 Vacuum too high -- waiting up to 40 seconds @20:34:12.44 SS.reconfigure :exhale @20:34:12.49 SS.hold @20:34:16.39 Intake @14.0psia, vacuum from 8.5 to 11.5psi @20:34:16.41 SS.reconfigure :inhaling 1.76ml/s @20:34:16.46 SS.seek 25ml @20:34:23.21 Vacuum too high -- waiting up to 40 seconds @20:34:23.22 SS.reconfigure :exhale @20:34:23.27 SS.hold @20:34:30.33 Intake @16.0psia, vacuum from 10.0 to 13.0psi @20:34:30.35 SS.reconfigure :inhaling 1.41ml/s @20:34:30.40 SS.seek 25ml @20:34:35.29 Stroke completed -- waiting for pressure to equalize ... @20:34:35.30 SS.reconfigure :exhale @20:34:35.35 SS.hold @20:34:46.37 Intake.select :close @20:34:46.53 SSV.dial :exhaust @20:34:47.65 Exhaust.select :open @20:34:47.83 SS.seek 0ml @20:34:56.62 Exhaust.select :close @20:34:56.73 SSV.dial CBV @20:34:56.75
CBV.dial SSV @20:34:57.56 Allowing 130s for 25.0ml of remaining 975.0ml @20:34:57.57 Intake.select :open @20:34:59.73 Intake @15.8psia, vacuum from 10.0 to 13.0psi @20:34:59.75 SS.reconfigure :inhaling 1.41ml/s @20:34:59.80 SS.seek 25ml @20:35:10.67 Vacuum too high -- waiting up to 40 seconds @20:35:10.69 SS.reconfigure :exhale @20:35:10.74 SS.hold @20:35:14.43 Intake @14.5psia, vacuum from 9.0 to 12.0psi @20:35:14.44 SS.reconfigure :inhaling 1.13ml/s @20:35:14.49 SS.seek 25ml @20:35:27.52 Stroke completed -- waiting for pressure to equalize ... @20:35:27.53 SS.reconfigure :exhale @20:35:27.58 SS.hold @20:35:42.28 Intake.select :close @20:35:42.43 SSV.dial :exhaust @20:35:43.20 Exhaust.select :open @20:35:43.31 SS.seek 0ml @20:35:52.07 Exhaust.select :close @20:35:52.18 SSV.dial CBV @20:35:52.20
CBV.dial SSV @20:35:53.01 Allowing 130s for 25.0ml of remaining 950.0ml @20:35:53.02 Intake.select :open @20:35:55.19 Intake @15.9psia, vacuum from 10.0 to 13.0psi @20:35:55.20 SS.reconfigure :inhaling 1.13ml/s @20:35:55.25 SS.seek 25ml @20:36:13.95 Vacuum too high -- waiting up to 40 seconds @20:36:13.96 SS.reconfigure :exhale @20:36:14.02 SS.hold @20:36:19.76 Intake @15.7psia, vacuum from 10.0 to 13.0psi @20:36:19.78 SS.reconfigure :inhaling 0.91ml/s @20:36:19.83 SS.seek 25ml @20:36:27.69 Stroke completed -- waiting for pressure to equalize ... @20:36:27.70 SS.reconfigure :exhale @20:36:27.75 SS.hold @20:36:38.94 Intake.select :close @20:36:39.09 SSV.dial :exhaust @20:36:39.85 Exhaust.select :open @20:36:39.97 SS.seek 0ml @20:36:48.66 Exhaust.select :close @20:36:48.78 SSV.dial CBV @20:36:48.82
CBV.dial SSV @20:36:49.57 Allowing 130s for 25.0ml of remaining 925.0ml @20:36:49.58 Intake.select :open @20:36:51.75 Intake @15.7psia, vacuum from 10.0 to 13.0psi @20:36:51.76 SS.reconfigure :inhaling 0.91ml/s @20:36:51.81 SS.seek 25ml @20:37:21.69 Stroke completed -- waiting for pressure to equalize ... @20:37:21.70 SS.reconfigure :exhale @20:37:21.75 SS.hold @20:37:34.41 Intake.select :close @20:37:34.57 SSV.dial :exhaust @20:37:35.33 Exhaust.select :open @20:37:35.45 SS.seek 0ml @20:37:44.37 Exhaust.select :close @20:37:44.47 SSV.dial CBV @20:37:44.49
CBV.dial SSV @20:37:45.30 Allowing 130s for 25.0ml of remaining 900.0ml @20:37:45.31 Intake.select :open @20:37:47.48 Intake @15.7psia, vacuum from 10.0 to 13.0psi @20:37:47.49 SS.reconfigure :inhaling 0.91ml/s @20:37:47.54 SS.seek 25ml @20:38:16.91 Stroke completed -- waiting for pressure to equalize ... @20:38:16.92 SS.reconfigure :exhale @20:38:16.97 SS.hold @20:38:29.61 Intake.select :close @20:38:29.76 SSV.dial :exhaust @20:38:30.52 Exhaust.select :open @20:38:30.63 SS.seek 0ml @20:38:39.60 Exhaust.select :close @20:38:39.71 SSV.dial CBV @20:38:39.73
CBV.dial SSV @20:38:40.51 Allowing 130s for 25.0ml of remaining 875.0ml @20:38:40.52 Intake.select :open @20:38:43.11 Intake @15.8psia, vacuum from 10.0 to 13.0psi @20:38:43.12 SS.reconfigure :inhaling 0.91ml/s @20:38:43.17 SS.seek 25ml @20:39:12.56 Stroke completed -- waiting for pressure to equalize ... @20:39:12.57 SS.reconfigure :exhale @20:39:12.62 SS.hold @20:39:27.03 Intake.select :close @20:39:27.18 SSV.dial :exhaust @20:39:27.96 Exhaust.select :open @20:39:28.07 SS.seek 0ml @20:39:37.05 Exhaust.select :close @20:39:37.16 SSV.dial CBV @20:39:37.18
CBV.dial SSV @20:39:37.99 Allowing 130s for 25.0ml of remaining 850.0ml @20:39:38.00 Intake.select :open @20:39:40.17 Intake @15.8psia, vacuum from 10.0 to 13.0psi @20:39:40.18 SS.reconfigure :inhaling 0.91ml/s @20:39:40.23 SS.seek 25ml @20:40:09.74 Stroke completed -- waiting for pressure to equalize ... @20:40:09.75 SS.reconfigure :exhale @20:40:09.79 SS.hold @20:40:26.30 Intake.select :close @20:40:26.45 SSV.dial :exhaust @20:40:27.21 Exhaust.select :open @20:40:27.32 SS.seek 0ml @20:40:36.06 Exhaust.select :close @20:40:36.18 SSV.dial CBV @20:40:36.21
CBV.dial SSV @20:40:36.97 Allowing 130s for 25.0ml of remaining 825.0ml @20:40:36.98 Intake.select :open @20:40:39.15 Intake @15.6psia, vacuum from 10.0 to 13.0psi @20:40:39.16 SS.reconfigure :inhaling 0.91ml/s @20:40:39.21 SS.seek 25ml @20:41:09.23 Stroke completed -- waiting for pressure to equalize ... @20:41:09.24 SS.reconfigure :exhale @20:41:09.29 SS.hold @20:41:25.60 Intake.select :close @20:41:25.76 SSV.dial :exhaust @20:41:26.52 Exhaust.select :open @20:41:26.63 SS.seek 0ml @20:41:35.52 Exhaust.select :close @20:41:35.62 SSV.dial CBV @20:41:35.64
CBV.dial SSV @20:41:36.42 Allowing 130s for 25.0ml of remaining 800.0ml @20:41:36.43 Intake.select :open @20:41:38.60 Intake @15.6psia, vacuum from 10.0 to 13.0psi @20:41:38.61 SS.reconfigure :inhaling 0.91ml/s @20:41:38.66 SS.seek 25ml @20:42:07.57 Vacuum too high -- waiting up to 40 seconds @20:42:07.58 SS.reconfigure :exhale @20:42:07.64 SS.hold @20:42:14.65 Intake @14.7psia, vacuum from 9.2 to 12.2psi @20:42:14.67 SS.reconfigure :inhaling 0.73ml/s @20:42:14.72 SS.seek 25ml @20:42:15.70 Stroke completed -- waiting for pressure to equalize ... @20:42:15.71 SS.reconfigure :exhale @20:42:16.11 SS.hold @20:42:24.76 Intake.select :close @20:42:24.91 SSV.dial :exhaust @20:42:25.69 Exhaust.select :open @20:42:25.80 SS.seek 0ml @20:42:34.53 Exhaust.select :close @20:42:34.64 SSV.dial CBV @20:42:34.66
CBV.dial SSV @20:42:35.47 Allowing 130s for 25.0ml of remaining 775.0ml @20:42:35.48 Intake.select :open @20:42:37.65 Intake @15.6psia, vacuum from 10.0 to 13.0psi @20:42:37.66 SS.reconfigure :inhaling 0.73ml/s @20:42:37.71 SS.seek 25ml @20:43:14.09 Stroke completed -- waiting for pressure to equalize ... @20:43:14.10 SS.reconfigure :exhale @20:43:14.15 SS.hold @20:43:30.36 Intake.select :close @20:43:30.51 SSV.dial :exhaust @20:43:31.28 Exhaust.select :open @20:43:31.40 SS.seek 0ml @20:43:40.33 Exhaust.select :close @20:43:40.43 SSV.dial CBV @20:43:40.45
CBV.dial SSV @20:43:41.23 Allowing 130s for 25.0ml of remaining 750.0ml @20:43:41.25 Intake.select :open @20:43:43.41 Intake @15.5psia, vacuum from 10.0 to 13.0psi @20:43:43.42 SS.reconfigure :inhaling 0.73ml/s @20:43:43.47 SS.seek 25ml @20:44:19.32 Stroke completed -- waiting for pressure to equalize ... @20:44:19.33 SS.reconfigure :exhale @20:44:19.38 SS.hold @20:44:35.68 Intake.select :close @20:44:35.84 SSV.dial :exhaust @20:44:36.62 Exhaust.select :open @20:44:36.74 SS.seek 0ml @20:44:45.50 Exhaust.select :close @20:44:45.61 SSV.dial CBV @20:44:45.63
CBV.dial SSV @20:44:46.42 Allowing 130s for 25.0ml of remaining 725.0ml @20:44:46.43 Intake.select :open @20:44:48.60 Intake @15.4psia, vacuum from 9.9 to 12.9psi @20:44:48.61 SS.reconfigure :inhaling 0.73ml/s @20:44:48.66 SS.seek 25ml @20:45:24.59 Stroke completed -- waiting for pressure to equalize ... @20:45:24.60 SS.reconfigure :exhale @20:45:24.65 SS.hold @20:45:42.90 Intake.select :close @20:45:43.47 SSV.dial :exhaust @20:45:44.25 Exhaust.select :open @20:45:44.36 SS.seek 0ml @20:45:53.12 Exhaust.select :close @20:45:53.23 SSV.dial CBV @20:45:53.25
CBV.dial SSV @20:45:54.04 Allowing 130s for 25.0ml of remaining 700.0ml @20:45:54.06 Intake.select :open @20:45:56.22 Intake @15.4psia, vacuum from 9.9 to 12.9psi @20:45:56.23 SS.reconfigure :inhaling 0.73ml/s @20:45:56.28 SS.seek 25ml @20:46:31.60 Vacuum too high -- waiting up to 40 seconds @20:46:31.61 SS.reconfigure :exhale @20:46:31.66 SS.hold @20:46:41.54 Intake @15.2psia, vacuum from 9.7 to 12.7psi @20:46:41.55 SS.reconfigure :inhaling 0.59ml/s @20:46:41.61 SS.seek 25ml @20:46:43.35 Stroke completed -- waiting for pressure to equalize ... @20:46:43.36 SS.reconfigure :exhale @20:46:43.41 SS.hold @20:46:53.24 Intake.select :close @20:46:53.39 SSV.dial :exhaust @20:46:54.16 Exhaust.select :open @20:46:54.27 SS.seek 0ml @20:47:03.01 Exhaust.select :close @20:47:03.12 SSV.dial CBV @20:47:03.14
CBV.dial SSV @20:47:03.93 Allowing 130s for 25.0ml of remaining 675.0ml @20:47:03.94 Intake.select :open @20:47:06.11 Intake @15.6psia, vacuum from 10.0 to 13.0psi @20:47:06.12 SS.reconfigure :inhaling 0.59ml/s @20:47:06.17 SS.seek 25ml @20:47:51.02 Stroke completed -- waiting for pressure to equalize ... @20:47:51.03 SS.reconfigure :exhale @20:47:51.08 SS.hold @20:48:09.43 Intake.select :close @20:48:09.58 SSV.dial :exhaust @20:48:10.35 Exhaust.select :open @20:48:10.47 SS.seek 0ml @20:48:19.46 Exhaust.select :close @20:48:19.57 SSV.dial CBV @20:48:19.60
CBV.dial SSV @20:48:20.37 Allowing 130s for 25.0ml of remaining 650.0ml @20:48:20.38 Intake.select :open @20:48:22.55 Intake @15.6psia, vacuum from 10.0 to 13.0psi @20:48:22.56 SS.reconfigure :inhaling 0.59ml/s @20:48:22.61 SS.seek 25ml @20:49:06.87 Stroke completed -- waiting for pressure to equalize ... @20:49:06.88 SS.reconfigure :exhale @20:49:06.92 SS.hold @20:49:27.77 Intake.select :close @20:49:27.92 SSV.dial :exhaust @20:49:28.68 Exhaust.select :open @20:49:28.80 SS.seek 0ml @20:49:37.54 Exhaust.select :close @20:49:37.65 SSV.dial CBV @20:49:37.67
CBV.dial SSV @20:49:38.46 Allowing 130s for 25.0ml of remaining 625.0ml @20:49:38.48 Intake.select :open @20:49:40.64 Intake @15.5psia, vacuum from 10.0 to 13.0psi @20:49:40.65 SS.reconfigure :inhaling 0.59ml/s @20:49:40.70 SS.seek 25ml @20:50:25.49 Stroke completed -- waiting for pressure to equalize ... @20:50:25.50 SS.reconfigure :exhale @20:50:25.55 SS.hold @20:50:47.98 Intake.select :close @20:50:48.13 SSV.dial :exhaust @20:50:48.91 Exhaust.select :open @20:50:49.45 SS.seek 0ml @20:50:58.40 Exhaust.select :close @20:50:58.51 SSV.dial CBV @20:50:58.53
CBV.dial SSV @20:50:59.32 Allowing 130s for 25.0ml of remaining 600.0ml @20:50:59.33 Intake.select :open @20:51:01.50 Intake @15.6psia, vacuum from 10.0 to 13.0psi @20:51:01.52 SS.reconfigure :inhaling 0.59ml/s @20:51:01.56 SS.seek 25ml @20:51:45.90 Stroke completed -- waiting for pressure to equalize ... @20:51:45.91 SS.reconfigure :exhale @20:51:45.95 SS.hold @20:52:12.53 Intake.select :close @20:52:12.68 SSV.dial :exhaust @20:52:13.47 Exhaust.select :open @20:52:14.00 SS.seek 0ml @20:52:22.65 Exhaust.select :close @20:52:22.75 SSV.dial CBV @20:52:22.77
CBV.dial SSV @20:52:23.57 Allowing 130s for 25.0ml of remaining 575.0ml @20:52:23.58 Intake.select :open @20:52:25.75 Intake @15.7psia, vacuum from 10.0 to 13.0psi @20:52:25.77 SS.reconfigure :inhaling 0.59ml/s @20:52:25.81 SS.seek 25ml @20:52:59.81 Vacuum too high -- waiting up to 40 seconds @20:52:59.82 SS.reconfigure :exhale @20:52:59.88 SS.hold @20:53:11.10 Intake @15.6psia, vacuum from 10.0 to 13.0psi @20:53:11.11 SS.reconfigure :inhaling 0.47ml/s @20:53:11.16 SS.seek 25ml @20:53:24.99 Stroke completed -- waiting for pressure to equalize ... @20:53:25.00 SS.reconfigure :exhale @20:53:25.04 SS.hold @20:53:43.80 Intake.select :close @20:53:43.96 SSV.dial :exhaust @20:53:44.72 Exhaust.select :open @20:53:44.84 SS.seek 0ml @20:53:53.88 Exhaust.select :close @20:53:53.99 SSV.dial CBV @20:53:54.01
CBV.dial SSV @20:53:54.78 Allowing 130s for 25.0ml of remaining 550.0ml @20:53:54.80 Intake.select :open @20:53:56.96 Intake @15.5psia, vacuum from 10.0 to 13.0psi @20:53:56.98 SS.reconfigure :inhaling 0.47ml/s @20:53:57.03 SS.seek 25ml @20:54:51.86 Stroke completed -- waiting for pressure to equalize ... @20:54:51.87 SS.reconfigure :exhale @20:54:51.92 SS.hold @20:55:18.38 Intake.select :close @20:55:18.54 SSV.dial :exhaust @20:55:19.32 Exhaust.select :open @20:55:19.44 SS.seek 0ml @20:55:28.55 Exhaust.select :close @20:55:28.66 SSV.dial CBV @20:55:28.68
CBV.dial SSV @20:55:29.47 Allowing 130s for 25.0ml of remaining 525.0ml @20:55:29.48 Intake.select :open @20:55:31.65 Intake @15.5psia, vacuum from 10.0 to 13.0psi @20:55:31.66 SS.reconfigure :inhaling 0.47ml/s @20:55:31.72 SS.seek 25ml @20:56:26.80 Stroke completed -- waiting for pressure to equalize ... @20:56:26.81 SS.reconfigure :exhale @20:56:26.85 SS.hold @20:56:57.55 Intake.select :close @20:56:57.71 SSV.dial :exhaust @20:56:58.47 Exhaust.select :open @20:56:58.58 SS.seek 0ml @20:57:07.34 Exhaust.select :close @20:57:07.45 SSV.dial CBV @20:57:07.47
CBV.dial SSV @20:57:08.25 Allowing 130s for 25.0ml of remaining 500.0ml @20:57:08.26 Intake.select :open @20:57:10.43 Intake @15.6psia, vacuum from 10.0 to 13.0psi @20:57:10.44 SS.reconfigure :inhaling 0.47ml/s @20:57:10.50 SS.seek 25ml @20:58:06.43 Stroke completed -- waiting for pressure to equalize ... @20:58:06.44 SS.reconfigure :exhale @20:58:06.49 SS.hold @20:58:37.55 Intake.select :close @20:58:37.70 SSV.dial :exhaust @20:58:38.47 Exhaust.select :open @20:58:38.58 SS.seek 0ml @20:58:47.30 Exhaust.select :close @20:58:47.41 SSV.dial CBV @20:58:47.43
CBV.dial SSV @20:58:48.22 Allowing 130s for 25.0ml of remaining 475.0ml @20:58:48.23 Intake.select :open @20:58:50.39 Intake @15.8psia, vacuum from 10.0 to 13.0psi @20:58:50.40 SS.reconfigure :inhaling 0.47ml/s @20:58:50.45 SS.seek 25ml @20:59:33.33 Vacuum too high -- waiting up to 40 seconds @20:59:33.35 SS.reconfigure :exhale @20:59:33.40 SS.hold @20:59:48.07 Intake @16.0psia, vacuum from 10.0 to 13.0psi @20:59:48.09 SS.reconfigure :inhaling 0.38ml/s @20:59:48.14 SS.seek 25ml @21:00:03.98 Stroke completed -- waiting for pressure to equalize ... @21:00:03.99 SS.reconfigure :exhale @21:00:04.04 SS.hold @21:00:26.49 Intake.select :close @21:00:26.65 SSV.dial :exhaust @21:00:27.40 Exhaust.select :open @21:00:27.52 SS.seek 0ml @21:00:36.25 Exhaust.select :close @21:00:36.35 SSV.dial CBV @21:00:36.38
CBV.dial SSV @21:00:37.17 Allowing 130s for 25.0ml of remaining 450.0ml @21:00:37.18 Intake.select :open @21:00:39.35 Intake @15.8psia, vacuum from 10.0 to 13.0psi @21:00:39.36 SS.reconfigure :inhaling 0.38ml/s @21:00:39.41 SS.seek 25ml @21:01:48.54 Stroke completed -- waiting for pressure to equalize ... @21:01:48.55 SS.reconfigure :exhale @21:01:48.60 SS.hold @21:02:21.62 Intake.select :close @21:02:21.78 SSV.dial :exhaust @21:02:22.55 Exhaust.select :open @21:02:22.66 SS.seek 0ml @21:02:31.59 Exhaust.select :close @21:02:31.70 SSV.dial CBV @21:02:31.72
CBV.dial SSV @21:02:32.49 Allowing 130s for 25.0ml of remaining 425.0ml @21:02:32.51 Intake.select :open @21:02:34.67 Intake @15.7psia, vacuum from 10.0 to 13.0psi @21:02:34.68 SS.reconfigure :inhaling 0.38ml/s @21:02:34.73 SS.seek 25ml @21:03:43.05 Stroke completed -- waiting for pressure to equalize ... @21:03:43.06 SS.reconfigure :exhale @21:03:43.11 SS.hold @21:04:17.82 Intake.select :close @21:04:17.98 SSV.dial :exhaust @21:04:18.76 Exhaust.select :open @21:04:18.87 SS.seek 0ml @21:04:27.88 Exhaust.select :close @21:04:27.99 SSV.dial CBV @21:04:28.01
CBV.dial SSV @21:04:28.82 Allowing 130s for 25.0ml of remaining 400.0ml @21:04:28.83 Intake.select :open @21:04:31.00 Intake @15.9psia, vacuum from 10.0 to 13.0psi @21:04:31.01 SS.reconfigure :inhaling 0.38ml/s @21:04:31.06 SS.seek 25ml @21:05:26.36 Vacuum too high -- waiting up to 40 seconds @21:05:26.37 SS.reconfigure :exhale @21:05:26.42 SS.hold @21:05:42.72 Intake @16.3psia, vacuum from 10.0 to 13.0psi @21:05:42.74 SS.reconfigure :inhaling 0.31ml/s @21:05:42.79 SS.seek 25ml @21:05:59.18 Stroke completed -- waiting for pressure to equalize ... @21:05:59.19 SS.reconfigure :exhale @21:05:59.23 SS.hold @21:06:23.79 Intake.select :close @21:06:23.94 SSV.dial :exhaust @21:06:24.69 Exhaust.select :open @21:06:24.81 SS.seek 0ml @21:06:33.61 Exhaust.select :close @21:06:33.73 SSV.dial CBV @21:06:33.77
CBV.dial SSV @21:06:34.52 Allowing 130s for 25.0ml of remaining 375.0ml @21:06:34.53 Intake.select :open @21:06:36.70 Intake @15.8psia, vacuum from 10.0 to 13.0psi @21:06:36.71 SS.reconfigure :inhaling 0.31ml/s @21:06:36.77 SS.seek 25ml @21:08:04.19 Stroke completed -- waiting for pressure to equalize ... @21:08:04.20 SS.reconfigure :exhale @21:08:04.25 SS.hold @21:08:39.40 Intake.select :close @21:08:39.56 SSV.dial :exhaust @21:08:40.32 Exhaust.select :open @21:08:40.43 SS.seek 0ml @21:08:49.22 Exhaust.select :close @21:08:49.33 SSV.dial CBV @21:08:49.35
CBV.dial SSV @21:08:50.16 Allowing 130s for 25.0ml of remaining 350.0ml @21:08:50.17 Intake.select :open @21:08:52.34 Intake @16.0psia, vacuum from 10.0 to 13.0psi @21:08:52.35 SS.reconfigure :inhaling 0.31ml/s @21:08:52.40 SS.seek 25ml @21:10:20.39 Stroke completed -- waiting for pressure to equalize ... @21:10:20.40 SS.reconfigure :exhale @21:10:20.45 SS.hold @21:10:57.15 Intake.select :close @21:10:57.31 SSV.dial :exhaust @21:10:58.08 Exhaust.select :open @21:10:58.19 SS.seek 0ml @21:11:07.20 Exhaust.select :close @21:11:07.31 SSV.dial CBV @21:11:07.33
CBV.dial SSV @21:11:08.13 Allowing 130s for 25.0ml of remaining 325.0ml @21:11:08.14 Intake.select :open @21:11:10.30 Intake @16.0psia, vacuum from 10.0 to 13.0psi @21:11:10.31 SS.reconfigure :inhaling 0.31ml/s @21:11:10.36 SS.seek 25ml @21:12:38.86 Stroke completed -- waiting for pressure to equalize ... @21:12:38.87 SS.reconfigure :exhale @21:12:38.91 SS.hold @21:13:18.15 SS.reconfigure :default @21:13:18.19 SS.hold @21:13:18.26 Intake.select :close @21:13:18.42 ShallowSampler::Clogged in MAIN -- Filtering too Slowly after 700.0ml @21:13:18.43 SSV.dial :exhaust @21:13:19.19 Exhaust.select :open @21:13:19.29 SS.reconfigure :exhale @21:13:19.34 SS.seek 0ml @21:13:28.40 Exhaust.select :close @21:13:28.51 Sampled 700.0ml @21:13:28.53 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @21:13:28.54 Intake.select :close @21:13:28.60 CSV.dial :waste @21:13:28.73 SSV.dial CBV,avoiding: :exhaust @21:13:28.76
CBV.dial SSV @21:13:28.88 CS.seek 0ml @21:13:28.95 CSV.dial :air @21:13:29.69 CS.seek 10ml @21:13:30.67
CTV.dial :air @21:13:31.42 delay 15 seconds @21:13:46.44 SSV.dial :spit,avoiding: :exhaust @21:13:47.79 CTV.dial CSV,avoiding: :intake @21:13:47.84
SS.seek 0ml @21:13:47.94 SSV.dial CBV,avoiding: :exhaust @21:13:54.77 SSV.dial between CBV and plug @21:13:55.24 CBV.dial between SSV and waste2 @21:13:55.63 CTV.dial CSV,avoiding: :air @21:13:55.66
CSV.dial CTV @21:13:56.16 CS.reconfigure :pressurize @21:13:56.21 CS.seek 0ml @21:14:15.02 BubblePoint: Intake @24.2psia, Outlet @0.4psia @21:14:15.03 CBV.dial SSV @21:14:15.38 SS.reconfigure :default @21:14:15.42 SS.seek 10ml @21:14:20.95 delay 15 seconds @21:14:36.08 Evacuated: Intake @23.9psia, Outlet @23.0psia @21:14:36.09 SSV.dial :spit,avoiding: :exhaust @21:14:37.14 SS.reconfigure :exhale @21:14:37.19 SS.seek 0ml @21:14:42.20 SS.reconfigure :fast @21:14:42.25 SS.seek 5ml @21:14:45.07 SSV.dial CBV,avoiding: :exhaust @21:14:45.09
CBV.dial SSV @21:14:46.44 CTV.dial :air,avoiding: :intake @21:14:47.80 CSV.dial CTV @21:14:47.85
SS.seek 10ml @21:14:49.02 CSV.dial :air @21:14:49.45 CS.reconfigure :fast @21:14:49.50 CS.seek 0ml @21:14:50.34
SSV.dial :spit,avoiding: :exhaust @21:14:51.69 SS.reconfigure :exhale @21:14:51.74 SS.seek 0ml @21:14:56.73 SS.reconfigure :fast @21:14:56.77 SS.seek 5ml @21:14:56.95 CS.stop @21:14:59.71 SSV.dial CBV,avoiding: :exhaust @21:14:59.73
CBV.dial SSV @21:15:02.09 SSV.dial :spit,avoiding: :exhaust @21:15:03.41 SS.reconfigure :default @21:15:03.46 SS.seek 0ml @21:15:06.70 SS.stop @21:15:06.74 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @21:15:06.75 Intake.select :close @21:15:06.82 CSV.dial :waste @21:15:07.38 SSV.dial CBV,avoiding: :exhaust @21:15:07.39
CBV.dial SSV @21:15:08.05 CS.seek 0ml @21:15:08.11 CSV.dial :air @21:15:08.73
CTV.dial :air @21:15:08.81 delay 15 seconds @21:15:08.86 CS.seek 10ml @21:15:23.84
SSV.dial :spit,avoiding: :exhaust @21:15:25.17 CTV.dial CSV,avoiding: :intake @21:15:25.21
SS.reconfigure :exhale @21:15:25.31 SS.seek 0ml @21:15:25.72 SSV.dial CBV,avoiding: :exhaust @21:15:33.23 SSV.dial between CBV and plug @21:15:33.72 CBV.dial between SSV and waste2 @21:15:34.12 CSV.dial between air and CTV @21:15:34.43 CS.seek 7.615ml @21:15:40.67 CTV.dial CSV,avoiding: :air @21:15:40.69
CSV.dial CTV @21:15:41.06 CS.reconfigure :pressurize @21:15:41.11 CS.seek 0ml @21:15:57.55 BubblePoint: Intake @24.2psia, Outlet @2.5psia @21:15:57.56 CBV.dial SSV @21:15:57.91 SS.reconfigure :default @21:15:57.95 SS.seek 10ml @21:16:03.54 delay 15 seconds @21:16:18.67 Evacuated: Intake @23.9psia, Outlet @23.1psia @21:16:18.68 SSV.dial :spit,avoiding: :exhaust @21:16:19.72 SS.reconfigure :exhale @21:16:19.77 SS.seek 0ml @21:16:24.91 SS.reconfigure :fast @21:16:24.96 SS.seek 5ml @21:16:27.89 SSV.dial CBV,avoiding: :exhaust @21:16:27.91
CBV.dial SSV @21:16:29.29 CTV.dial :air,avoiding: :intake @21:16:30.64 CSV.dial CTV @21:16:30.67
SS.seek 10ml @21:16:31.87 CSV.dial :air @21:16:32.33 CS.reconfigure :fast @21:16:32.38 CS.seek 0ml @21:16:33.28
SSV.dial :spit,avoiding: :exhaust @21:16:34.63 SS.reconfigure :exhale @21:16:34.67 SS.seek 0ml @21:16:36.38 CS.stop @21:16:39.97
SS.reconfigure :fast @21:16:40.45 SS.seek 5ml @21:16:43.05 SSV.dial CBV,avoiding: :exhaust @21:16:43.07
CBV.dial SSV @21:16:45.44 SSV.dial :spit,avoiding: :exhaust @21:16:46.78 SS.reconfigure :default @21:16:46.83 SS.seek 0ml @21:16:50.02 SS.stop @21:16:50.07 Lysing: Adding 2.0ml AqMeOH @21:16:50.08 CBV.dial between CSV and waste1,avoiding: :DBV @21:16:51.59 CTV.dial CSV @21:16:52.92 CSV.dial CSR @21:16:53.83 Pulling 1ml of CSR.AqMeOH @21:16:53.84 CSR.select :AqMeOH @21:16:53.95 CS.reconfigure :default @21:16:54.00 CS.seek 1ml @21:16:57.38 delay 5 seconds @21:17:02.40 CSR.select :air @21:17:02.57 CS.seek 6ml @21:17:16.23 delay 2 seconds @21:17:18.24 CSV.dial :waste2 @21:17:18.72 CS.reconfigure :fast @21:17:18.77 CS.seek 0ml @21:17:33.20 CSV.dial CSR @21:17:33.65 Pulling 2ml of CSR.AqMeOH @21:17:33.66 CSR.select :AqMeOH @21:17:33.78 CS.reconfigure :default @21:17:33.82 CS.seek 2ml @21:17:40.01 delay 5 seconds @21:17:45.03 CSR.select :air @21:17:45.20 CS.seek 4.999ml @21:17:53.88 delay 2 seconds @21:17:55.90 CSV.dial CTV @21:17:56.34 CS.seek 1ml @21:18:07.71 Before lysing Collection Puck Heater at 12.7C/27.1psia @21:18:07.73 Heating Puck.from 3, :sh1 in Collection Clamp to 70C for 10:00 @21:18:07.75 CH.seek 70C,8:00 @21:20:24.65 CH.hold 10:00,70C @21:20:24.85 Begun lysing Collection Puck Heater at 68.1C/33.8psia @21:30:25.31 Ended lysing Collection Puck Heater at 70C/33.7psia @21:30:25.32 CS.seek 0ml @21:30:28.69 CTV.dial between spare and air,avoiding: :intake @21:30:29.72 CSV.dial :air @21:30:30.18 CS.seek 5ml @21:30:43.78 CSV.dial CTV @21:30:44.23 delay 2 seconds @21:30:46.25 CSV.dial :air @21:30:46.70 delay 2 seconds @21:30:48.72 CSV.dial CTV @21:30:49.17 delay 2 seconds @21:30:51.19 CSV.dial :air @21:30:51.64 delay 2 seconds @21:30:53.66 CS.seek 0ml @21:31:07.27 Puck @66.2psia after final lysis pressurization @21:31:07.28 Recovering Lysate into Collection syringe @21:31:07.29 CSV.dial CBV @21:31:08.31 CBV.dial CSV @21:31:08.68 CS.seek 5.001ml @21:31:22.28 delay 5 seconds @21:31:27.30 CTV.dial :air @21:31:27.82 CS.seek 7.001ml @21:31:33.72 delay 5 seconds @21:31:38.73 CSV.dial :air2 @21:31:39.18 CS.seek 10ml @21:31:47.72 CSV.dial between air and CTV,avoiding: CBV @21:31:48.88 Lysing Complete @21:31:48.95 Storing sh1 puck in Collection Clamp to tube 2 @21:31:49.12 SC.reconfigure :tight @21:31:49.16 SC.seek 2 @21:31:52.66 SC.configure :limp @21:31:52.88 Elbow.reconfigure :tight @21:31:52.93 Elbow.seek 2 @21:31:54.63 Forearm.reconfigure :tight @21:31:54.68 Forearm.seek 2 + 450 counts @21:31:55.71 SE.reconfigure :pick @21:31:55.77 SE.seek :beamBreak @21:31:57.35 SE.configure :default @21:31:57.51 Elbow.reconfigure :limp @21:31:57.56 Forearm.reconfigure :loose @21:31:57.60 Forearm.seek 2 @21:31:58.26 Elbow.hold @21:31:58.33 Elbow.reconfigure :tight @21:31:58.43 SE.seek 22.11 Pucks below Top Plate @21:31:59.01 Forearm.force -20 @21:31:59.05 Forearm.seek 2 + 312 counts @21:31:59.41 Forearm.reconfigure :tight @21:31:59.46 Forearm.seek 2 + 450 counts @21:31:59.72 SE.seek 23.11 Pucks below Top Plate @21:32:00.28 Forearm.reconfigure :loose @21:32:00.33 Forearm.seek :retract @21:32:01.25 Elbow.seek CC @21:32:02.92 Elbow.reconfigure :loose @21:32:03.02 Forearm.seek CC @21:32:04.02 Hand.close @21:32:04.59 CC.seek :open @21:32:09.33 Forearm.seek :retract @21:32:10.32 Elbow.reconfigure :tight @21:32:10.37 Elbow.seek 2 @21:32:12.14 Elbow.reconfigure :limp @21:32:12.19 Forearm.seek 2 @21:32:13.18 Elbow.hold @21:32:13.25 Elbow.reconfigure :tight @21:32:13.29 Hand.open @21:32:14.08 Forearm.force -20 @21:32:14.13 Forearm.seek 2 + 310 counts @21:32:14.50 Forearm.reconfigure :tight @21:32:14.55 Forearm.seek 2 + 450 counts @21:32:14.86 SE.seek :down @21:32:15.99
Forearm.reconfigure :loose @21:32:16.04 Forearm.seek :retract @21:32:16.42 SE.stop @21:32:17.00
Tranferring DA @21:32:17.01 Beginning samplePassC @21:32:17.02 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @21:32:17.21 Elbow.seek FlushPuck::Garage @21:32:19.12 Elbow.reconfigure :loose @21:32:19.22 Forearm.seek :garage @21:32:20.18 Hand.close @21:32:20.96 Forearm.seek :retract @21:32:21.90 Elbow.reconfigure :tight @21:32:21.95 Elbow.seek CC @21:32:24.96 Elbow.reconfigure :loose @21:32:25.06 Forearm.reconfigure :tight @21:32:25.11 Forearm.seek CC @21:32:26.41 CC.seek :closed @21:32:30.93 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.40 @21:32:31.00 Hand.open @21:32:31.65 Forearm.reconfigure :loose @21:32:31.70 Forearm.seek :retract @21:32:32.65 PSV.dial PSR @21:32:34.07 Pulling 1.5ml of PSR.flush @21:32:34.08 PSR.select :flush @21:32:34.20 PS.seek 1.5ml @21:32:50.81 delay 5 seconds @21:32:55.83 PSV.dial :waste1 @21:32:56.30 PS.seek 0ml @21:33:12.43 PSV.dial PSR @21:33:12.90 PSR.select :air @21:33:13.02 PS.seek 2.5ml @21:33:39.35 PRV.dial CBV,avoiding: :ab2 @21:33:39.39
CBV.dial PRV @21:33:41.12 PSV.dial :mixloop @21:33:42.17 CTV.dial :air,avoiding: :intake @21:33:42.24 PTV.dial :PRVmixing @21:33:43.61 PS.seek 0ml @21:34:09.90 PSV.dial :air @21:34:10.38 PS.seek 2.5ml @21:34:36.71 PSV.dial :mixloop @21:34:37.19 PS.seek 0ml @21:35:03.53 PSV.dial :air @21:35:04.00 PS.seek 2.5ml @21:35:30.37 PSV.dial :mixloop @21:35:30.85 PS.seek 0ml @21:35:57.22 Garaging FlushPuck in Collection Clamp @21:35:57.29 Elbow.reconfigure :tight @21:35:57.33 Elbow.seek CC @21:35:57.63 Elbow.reconfigure :loose @21:35:57.72 Forearm.seek CC @21:35:58.79 Hand.close @21:35:59.34 CC.seek :open @21:36:03.95 Forearm.seek :retract @21:36:04.95 Elbow.reconfigure :tight @21:36:04.99 Elbow.seek :garage @21:36:07.94 Elbow.reconfigure :loose @21:36:08.04 Forearm.reconfigure :tight @21:36:08.08 Forearm.seek :garage @21:36:09.10 Hand.open @21:36:09.77 Forearm.reconfigure :loose @21:36:10.19 Forearm.seek :retract @21:36:11.26 PSV.dial :mixloop @21:36:11.32 PTV.dial :PRVmixing @21:36:11.41 PRV.dial CBV @21:36:11.44
CBV.dial PRV @21:36:11.61 CTV.dial :air,avoiding: :intake @21:36:11.69 Loading lyfil puck from tube 3 into Collection Clamp @21:36:11.92 SC.reconfigure :tight @21:36:11.97 SC.seek 3 @21:36:15.55 SC.configure :limp @21:36:15.76 Elbow.reconfigure :tight @21:36:15.81 Elbow.seek 3 @21:36:17.72 Forearm.reconfigure :tight @21:36:17.77 Forearm.seek 3 + 450 counts @21:36:18.80 SE.reconfigure :pick @21:36:18.86 SE.seek :beamBreak @21:36:21.47 SE.configure :default @21:36:21.63 Elbow.seek 3 @21:36:21.82 Forearm.seek 3 + 300 counts @21:36:22.02 Forearm.reconfigure :loose @21:36:22.07 Hand.close @21:36:22.72 SE.seek :down @21:36:22.93
Forearm.seek :retract @21:36:23.88 Elbow.seek CC @21:36:24.92 SE.stop @21:36:25.56
Elbow.reconfigure :loose @21:36:25.66 Forearm.reconfigure :tight @21:36:25.71 Forearm.seek CC @21:36:27.43 CC.seek :closed @21:36:31.96 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.15 @21:36:31.97 Hand.open @21:36:32.65 Forearm.reconfigure :loose @21:36:32.69 Forearm.seek :retract @21:36:33.70 CTV.dial CSV,avoiding: :intake @21:36:35.07 CSV.dial CTV @21:36:35.41 PS.seek 2.5ml @21:37:01.84 CS.seek 4.5ml @21:37:16.71 delay 8 seconds @21:37:24.73 PSV.dial :air @21:37:25.20 delay 2 seconds @21:37:27.21 PSV.dial :waste2 @21:37:27.97 PS.seek 0ml @21:37:54.24 CBV.dial between PRV and waste1 @21:37:54.57 CSV.dial :air @21:37:55.11 CS.seek 10ml @21:38:09.96 CSV.dial CTV @21:38:10.42 CS.seek 0ml @21:38:37.05 CBV.dial :waste1 @21:38:37.37 delay 5 seconds @21:38:42.39 CTV.dial :air,avoiding: :intake @21:38:43.80 Storing lyfil puck in Collection Clamp to tube 2 @21:38:43.98 SC.reconfigure :tight @21:38:44.03 SC.seek 2 @21:38:47.55 SC.configure :limp @21:38:47.77 Elbow.reconfigure :tight @21:38:47.82 Elbow.seek 2 @21:38:50.04 Forearm.reconfigure :tight @21:38:50.09 Forearm.seek 2 + 450 counts @21:38:51.23 SE.reconfigure :pick @21:38:51.29 SE.seek :beamBreak @21:38:52.92 SE.configure :default @21:38:53.08 Elbow.reconfigure :limp @21:38:53.13 Forearm.reconfigure :loose @21:38:53.18 Forearm.seek 2 @21:38:53.38 Elbow.hold @21:38:53.44 Elbow.reconfigure :tight @21:38:53.54 SE.seek 23.1 Pucks below Top Plate @21:38:54.08 Forearm.force -20 @21:38:54.12 Forearm.seek 2 + 319 counts @21:38:54.42 Forearm.reconfigure :tight @21:38:54.47 Forearm.seek 2 + 450 counts @21:38:54.68 SE.seek 24.1 Pucks below Top Plate @21:38:55.24 Forearm.reconfigure :loose @21:38:55.29 Forearm.seek :retract @21:38:56.22 Elbow.seek CC @21:38:57.96 Elbow.reconfigure :loose @21:38:58.06 Forearm.seek CC @21:38:59.00 Hand.close @21:38:59.59 CC.seek :open @21:39:04.31 Forearm.seek :retract @21:39:05.26 Elbow.reconfigure :tight @21:39:05.30 Elbow.seek 2 @21:39:07.53 Elbow.reconfigure :limp @21:39:07.58 Forearm.seek 2 @21:39:08.57 Elbow.hold @21:39:08.63 Elbow.reconfigure :tight @21:39:08.68 Hand.open @21:39:09.46 Forearm.force -20 @21:39:09.50 Forearm.seek 2 + 307 counts @21:39:09.79 Forearm.reconfigure :tight @21:39:09.84 Forearm.seek 2 + 450 counts @21:39:10.12 SE.seek :down @21:39:11.26 SE.stop @21:39:11.29
Forearm.reconfigure :loose @21:39:11.37 Forearm.seek :retract @21:39:12.32 PSV.dial :air @21:39:13.20 PS.seek 2.5ml @21:39:39.62 delay 1 second @21:39:40.63 PSV.dial :mixloop @21:39:41.12 delay 1 second @21:39:42.13 PSV.dial :air @21:39:42.60 delay 1 second @21:39:43.62 PSV.dial :mixloop @21:39:44.09 delay 1 second @21:39:45.10 PSV.dial :air @21:39:45.57 delay 1 second @21:39:46.59 PS.seek 0ml @21:40:12.96 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @21:40:13.14 Elbow.seek FlushPuck::Garage @21:40:15.01 Elbow.reconfigure :loose @21:40:15.11 Forearm.seek :garage @21:40:16.30 Hand.close @21:40:17.07 Forearm.seek :retract @21:40:18.09 Elbow.reconfigure :tight @21:40:18.14 Elbow.seek PC @21:40:19.85 Elbow.reconfigure :loose @21:40:19.95 Forearm.reconfigure :tight @21:40:20.00 Forearm.seek PC @21:40:21.20 PC.seek :closed @21:40:25.81 Processing Clamp onSpring/offSpring for 16/26 samples, on/off torque 1.61 @21:40:25.88 Hand.open @21:40:26.51 Forearm.reconfigure :loose @21:40:26.56 Forearm.seek :retract @21:40:27.56 PRV.dial :air @21:40:28.02 PSV.dial :mixloop @21:40:28.56 PS.seek 0.3ml @21:40:32.52 PSV.dial PSR @21:40:33.59 Pulling 0.4ml of PSR.air @21:40:33.60 PSR.select :air @21:40:33.62 PS.seek 0.7ml @21:40:38.46 delay 3 seconds @21:40:41.48 PSR.select :air @21:40:41.50 PS.seek 2.5ml @21:41:00.71 delay 3 seconds @21:41:03.73 PSV.dial :mixloop @21:41:04.79 PTV.dial :mixing @21:41:05.55 PBV.dial :waste2 @21:41:06.50 PS.seek 0ml @21:41:32.80 Garaging FlushPuck in Processing Clamp @21:41:32.87 Elbow.reconfigure :tight @21:41:32.92 Elbow.seek PC @21:41:33.04 Elbow.reconfigure :loose @21:41:33.14 Forearm.seek PC @21:41:34.13 Hand.close @21:41:34.71 PC.seek :open @21:41:39.79 Forearm.seek :retract @21:41:40.88 Elbow.reconfigure :tight @21:41:40.93 Elbow.seek :garage @21:41:42.29 Elbow.reconfigure :loose @21:41:42.39 Forearm.reconfigure :tight @21:41:42.44 Forearm.seek :garage @21:41:43.48 Hand.open @21:41:44.14 Forearm.reconfigure :loose @21:41:44.19 Forearm.seek :retract @21:41:45.16 Loading da puck from tube 3 into Processing Clamp @21:41:45.39 SC.reconfigure :tight @21:41:45.44 SC.seek 3 @21:41:48.98 SC.configure :limp @21:41:49.20 Elbow.reconfigure :tight @21:41:49.24 Elbow.seek 3 @21:41:51.12 Forearm.reconfigure :tight @21:41:51.17 Forearm.seek 3 + 450 counts @21:41:52.25 SE.reconfigure :pick @21:41:52.31 SE.seek :beamBreak @21:41:54.92 SE.configure :default @21:41:55.08 Elbow.seek 3 @21:41:55.27 Forearm.seek 3 + 300 counts @21:41:55.53 Forearm.reconfigure :loose @21:41:55.58 Hand.close @21:41:56.23 SE.seek :down @21:41:56.43
Forearm.seek :retract @21:41:57.36 Elbow.seek PC @21:41:58.53 SE.stop @21:42:00.00
Elbow.reconfigure :loose @21:42:00.10 Forearm.reconfigure :tight @21:42:00.52 Forearm.seek PC @21:42:01.83 PC.seek :closed @21:42:06.47 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.62 @21:42:06.48 Hand.open @21:42:07.14 Forearm.reconfigure :loose @21:42:07.19 Forearm.seek :retract @21:42:08.20 Heating Puck.from 3, :da in Processing Clamp to 28C for 2:00:00 @21:42:08.21 PH.seek 28C,5:00 @21:42:51.97 PH.hold 2:00:00,28C @21:42:52.03 delay 1:30 @21:44:22.06 PSR.select :air @21:44:22.07 PSV.dial PSR @21:44:23.13 Pulling 0.5ml of PSR.wash @21:44:23.14 PSR.select :wash @21:44:23.26 PS.seek 0.5ml @21:44:29.17 delay 5 seconds @21:44:34.19 PSR.select :air @21:44:34.32 PS.seek 2ml @21:44:50.52 PTV.dial :puck @21:44:51.27 PSV.dial PTV @21:44:52.03 PBV.dial :waste1 @21:44:53.44 PS.seek 1.5ml @21:44:59.36 delay 3 seconds @21:45:02.38 PBV.dial between PSV and waste1 @21:45:02.74 PS.seek 0ml @21:45:18.80 delay 5:00 @21:50:18.82 recoverPSRreagent08 @21:50:18.83 PTV.dial :puck @21:50:18.90 PBV.dial :waste1 @21:50:19.28 delay 30 seconds @21:50:49.30 PBV.dial between PSV and waste1 @21:50:49.68 PSR.select :air @21:50:49.70 PSV.dial PSR @21:50:50.47 PS.reconfigure :fast @21:50:50.52 PS.seek 2.5ml @21:51:14.42 PSV.dial PTV @21:51:15.18 delay 5 seconds @21:51:20.20 PSV.dial PSR @21:51:20.97 delay 5 seconds @21:51:25.98 PSV.dial PTV @21:51:26.75 delay 5 seconds @21:51:31.77 PSV.dial :air @21:51:32.64 PS.seek 0ml @21:51:55.73 PTV.dial :PRVmixing,via: PRV @21:51:57.06 PSV.dial :mixloop @21:51:57.51 PRV.dial :ab1 @21:51:58.27 PS.reconfigure :slow @21:51:58.31 PS.seek 0.15ml @21:52:01.92 delay 2 seconds @21:52:03.94 CBV.dial PRV @21:52:03.97
PRV.dial CBV @21:52:04.52 PS.seek 0.35ml @21:52:09.07 delay 2 seconds @21:52:11.52 CBV.dial between PRV and PSV @21:52:11.85 PRV.dial :air @21:52:12.31 PS.seek 0.9ml @21:52:24.01 PRV.dial :waste2 @21:52:24.77 PS.seek 0.5ml @21:52:33.51 addPMLReagent08 @21:52:33.52 PSV.dial :air @21:52:34.14 PS.reconfigure :default @21:52:34.19 PS.seek 2ml @21:52:50.32 PSV.dial :PML @21:52:50.77 PTV.dial :mixing @21:52:51.54 PBV.dial :waste1 @21:52:51.98 PS.seek 1.85ml @21:52:54.41 PBV.dial between PSV and waste1 @21:52:54.80 PS.seek 0ml @21:53:14.51 delay 2 seconds @21:53:16.53 Beginning samplePassC @21:53:16.54 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @21:53:16.72 Elbow.reconfigure :tight @21:53:16.77 Elbow.seek FlushPuck::Garage @21:53:18.14 Elbow.reconfigure :loose @21:53:18.24 Forearm.seek :garage @21:53:19.26 Hand.close @21:53:19.95 Forearm.seek :retract @21:53:20.92 Elbow.reconfigure :tight @21:53:20.96 Elbow.seek CC @21:53:23.89 Elbow.reconfigure :loose @21:53:23.99 Forearm.reconfigure :tight @21:53:24.04 Forearm.seek CC @21:53:25.27 CC.seek :closed @21:53:29.84 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.33 @21:53:29.92 Hand.open @21:53:30.64 Forearm.reconfigure :loose @21:53:30.69 Forearm.seek :retract @21:53:31.62 PSV.dial PSR @21:53:32.81 Pulling 1.5ml of PSR.flush PSR.select :flush @21:53:32.94 PS.seek 1.5ml @21:53:49.16 delay 5 seconds @21:53:54.18 PSV.dial :waste1 @21:53:54.69 PS.seek 0ml @21:54:10.85 PSV.dial PSR @21:54:11.35 PSR.select :air @21:54:11.47 PS.seek 2.5ml @21:54:37.89 PRV.dial CBV,avoiding: :ab2 @21:54:37.91
CBV.dial PRV @21:54:39.54 PSV.dial :mixloop @21:54:40.59 CTV.dial :air,avoiding: :intake @21:54:40.66 PTV.dial :PRVmixing @21:54:41.44 PS.seek 0ml @21:55:07.76 PSV.dial :air @21:55:08.36 PS.seek 2.5ml @21:55:34.69 PSV.dial :mixloop @21:55:35.15 PS.seek 0ml @21:56:01.47 PSV.dial :air @21:56:02.07 PS.seek 2.5ml @21:56:28.47 PSV.dial :mixloop @21:56:28.93 PS.seek 0ml @21:56:55.35 Garaging FlushPuck in Collection Clamp @21:56:55.42 Elbow.reconfigure :tight @21:56:55.47 Elbow.seek CC @21:56:55.72 Elbow.reconfigure :loose @21:56:55.82 Forearm.seek CC @21:56:56.80 Hand.close @21:56:57.45 CC.seek :open @21:57:02.06 Forearm.seek :retract @21:57:03.49 Elbow.reconfigure :tight @21:57:03.54 Elbow.seek :garage @21:57:06.49 Elbow.reconfigure :loose @21:57:06.59 Forearm.reconfigure :tight @21:57:06.64 Forearm.seek :garage @21:57:07.70 Hand.open @21:57:08.38 Forearm.reconfigure :loose @21:57:08.43 Forearm.seek :retract @21:57:09.45 delayUntil "22:01:16.52" @21:57:09.47 Whole Cell Archive sampling 1000ml @21:57:09.49 Loading wcr puck from tube 3 into Collection Clamp @21:57:09.73 SC.reconfigure :tight @21:57:09.78 SC.seek 3 @21:57:09.85 SC.configure :limp @21:57:10.07 Elbow.reconfigure :tight @21:57:10.12 Elbow.seek 3 @21:57:12.09 Forearm.reconfigure :tight @21:57:12.15 Forearm.seek 3 + 450 counts @21:57:13.16 SE.reconfigure :pick @21:57:13.22 SE.seek :beamBreak @21:57:16.01 SE.configure :default @21:57:16.17 Elbow.seek 3 @21:57:16.36 Forearm.seek 3 + 300 counts @21:57:16.61 Forearm.reconfigure :loose @21:57:16.66 Hand.close @21:57:17.33 SE.seek :down @21:57:17.54 Forearm.seek :retract @21:57:18.93 Elbow.seek CC @21:57:19.69 SE.stop @21:57:20.56 Elbow.reconfigure :loose @21:57:20.67 Forearm.reconfigure :tight @21:57:20.72 Forearm.seek CC @21:57:22.05 CC.seek :closed @21:57:26.58 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.30 @21:57:26.59 Hand.open @21:57:27.25 Forearm.reconfigure :loose @21:57:27.30 Forearm.seek :retract @21:57:28.31 collectSample 1000ml w/bubble point of 35psi @21:57:28.32 Priming 7ml @21:57:28.33 Intake.select :close @21:57:28.34 Exhaust.select :close @21:57:28.35 SS.hold @21:57:28.56 Calibrating pressure sensors @21:57:28.57 CTV.dial :air @21:57:28.65 SSV.dial :spit,avoiding: :exhaust @21:57:28.74 SS.reconfigure :fast @21:57:28.79 SS.seek 5ml @21:57:31.69 SSV.dial CBV @21:57:31.71 CBV.dial SSV @21:57:33.09 delay 3.8 seconds @21:57:37.03 Sensor limits: Intake max 106.8psia; Outlet max 98.5psia @21:57:37.04 SSV.dial :spit @21:57:38.38 SS.seek 0ml @21:57:40.96 SSV.dial CBV @21:57:42.30 CTV.dial :intake @21:57:43.07 SSV.dial CBV @21:57:43.09 CBV.dial SSV @21:57:43.62 Allowing 40s for 7.0ml of remaining 7.0ml @21:57:43.63 Intake.select :open @21:57:45.80 Intake @14.9psia, vacuum from 9.4 to 12.4psi @21:57:45.82 SS.reconfigure :inhaling 2.20ml/s @21:57:45.87 SS.seek 7ml @21:57:52.40 Stroke completed -- waiting for pressure to equalize ... @21:57:52.41 SS.reconfigure :exhale @21:57:52.46 SS.hold @21:58:05.15 Intake.select :close @21:58:05.31 SSV.dial :exhaust @21:58:06.07 Exhaust.select :open @21:58:06.19 SS.seek 0ml @21:58:10.12 Exhaust.select :close @21:58:10.23 Sampling 1000.0ml @21:58:10.25 Intake.select :close @21:58:10.26 Exhaust.select :close @21:58:10.27 SS.reconfigure :default @21:58:10.31 SS.hold @21:58:10.96 CTV.dial :intake @21:58:11.06 SSV.dial CBV @21:58:11.10 CBV.dial SSV @21:58:11.84 Allowing 130s for 25.0ml of remaining 1000.0ml @21:58:11.86 Intake.select :open @21:58:14.02 Intake @15.2psia, vacuum from 9.7 to 12.7psi @21:58:14.04 SS.reconfigure :inhaling 2.20ml/s @21:58:14.09 SS.seek 25ml @21:58:21.77 Vacuum too high -- waiting up to 40 seconds @21:58:21.78 SS.reconfigure :exhale @21:58:21.84 SS.hold @21:58:28.77 Intake @15.3psia, vacuum from 9.8 to 12.8psi @21:58:28.79 SS.reconfigure :inhaling 1.76ml/s @21:58:28.84 SS.seek 25ml @21:58:38.10 Stroke completed -- waiting for pressure to equalize ... @21:58:38.11 SS.reconfigure :exhale @21:58:38.16 SS.hold @21:59:02.84 Intake.select :close @21:59:03.00 SSV.dial :exhaust @21:59:03.76 Exhaust.select :open @21:59:03.88 SS.seek 0ml @21:59:12.71 Exhaust.select :close @21:59:12.83 SSV.dial CBV @21:59:12.86 CBV.dial SSV @21:59:13.64 Allowing 130s for 25.0ml of remaining 975.0ml @21:59:13.65 Intake.select :open @21:59:15.82 Intake @16.1psia, vacuum from 10.0 to 13.0psi @21:59:15.84 SS.reconfigure :inhaling 1.76ml/s @21:59:15.89 SS.seek 25ml @21:59:24.92 Vacuum too high -- waiting up to 40 seconds @21:59:24.94 SS.reconfigure :exhale @21:59:25.40 SS.hold @21:59:31.19 SS.reconfigure :inhaling 1.41ml/s @21:59:31.24 SS.seek 25ml @21:59:39.67 Vacuum too high -- waiting up to 40 seconds @21:59:39.69 SS.reconfigure :exhale @21:59:39.74 SS.hold @21:59:46.01 Intake @16.6psia, vacuum from 10.0 to 13.0psi @21:59:46.03 SS.reconfigure :inhaling 1.13ml/s @21:59:46.08 SS.seek 25ml @21:59:49.27 Stroke completed -- waiting for pressure to equalize ... @21:59:49.29 SS.reconfigure :exhale @21:59:49.33 SS.hold @22:00:07.71 Intake.select :close @22:00:07.87 SSV.dial :exhaust @22:00:08.63 Exhaust.select :open @22:00:08.75 SS.seek 0ml @22:00:17.94 Exhaust.select :close @22:00:18.05 SSV.dial CBV @22:00:18.08 CBV.dial SSV @22:00:18.85 Allowing 130s for 25.0ml of remaining 950.0ml @22:00:18.87 Intake.select :open @22:00:21.04 Intake @16.0psia, vacuum from 10.0 to 13.0psi @22:00:21.06 SS.reconfigure :inhaling 1.13ml/s @22:00:21.11 SS.seek 25ml @22:00:45.67 Vacuum too high -- waiting up to 40 seconds @22:00:45.69 SS.reconfigure :exhale @22:00:45.74 SS.hold @22:00:55.69 Intake @18.3psia, vacuum from 10.0 to 13.0psi @22:00:55.71 SS.reconfigure :inhaling 0.91ml/s @22:00:55.76 SS.seek 25ml @22:00:56.24 Stroke completed -- waiting for pressure to equalize ... @22:00:56.25 SS.reconfigure :exhale @22:00:56.30 SS.hold @22:01:10.95 Intake.select :close @22:01:11.11 SSV.dial :exhaust @22:01:11.87 Exhaust.select :open @22:01:11.99 SS.seek 0ml @22:01:16.53 recoverPRVreagent08 @22:01:16.54 PTV.dial :mixing @22:01:17.30 PBV.dial :waste1 @22:01:17.67 delay 30 seconds @22:01:21.07 Exhaust.select :close @22:01:21.18 SSV.dial CBV @22:01:21.22 CBV.dial SSV @22:01:21.99 Allowing 130s for 25.0ml of remaining 925.0ml @22:01:22.01 Intake.select :open @22:01:24.17 Intake @15.9psia, vacuum from 10.0 to 13.0psi @22:01:24.19 SS.reconfigure :inhaling 0.91ml/s @22:01:24.24 SS.seek 25ml @22:01:47.69 PBV.dial between PSV and waste1 @22:01:48.05 PSV.dial :air @22:01:48.52 PS.reconfigure :fast @22:01:48.57 PS.seek 2.5ml @22:01:53.93 Stroke completed -- waiting for pressure to equalize ... @22:01:54.39 SS.reconfigure :exhale @22:01:54.43 SS.hold @22:02:12.68 PSV.dial :mixloop @22:02:13.17 delay 2 seconds @22:02:14.89 Intake.select :close @22:02:15.05 SSV.dial :exhaust @22:02:15.19 PSV.dial :air @22:02:15.66 delay 2 seconds @22:02:15.85 Exhaust.select :open @22:02:15.97 SS.seek 0ml @22:02:17.68 PSV.dial :mixloop @22:02:18.17 delay 2 seconds @22:02:20.19 PSV.dial :air @22:02:20.79 PS.seek 0ml @22:02:25.51 Exhaust.select :close @22:02:25.62 SSV.dial CBV @22:02:25.65 CBV.dial SSV @22:02:26.41 Allowing 130s for 25.0ml of remaining 900.0ml @22:02:26.43 Intake.select :open @22:02:28.61 Intake @15.9psia, vacuum from 10.0 to 13.0psi @22:02:28.63 SS.reconfigure :inhaling 0.91ml/s @22:02:28.67 SS.seek 25ml @22:02:44.50 washCycleDA #1 of 2 @22:02:44.51 PSR.select :air @22:02:44.53 PSV.dial PSR @22:02:45.89 Pulling 0.5ml of PSR.wash @22:02:45.90 PSR.select :wash @22:02:46.01 PS.reconfigure :default @22:02:46.06 PS.seek 0.5ml @22:02:51.97 delay 5 seconds @22:02:56.99 PSR.select :air @22:02:57.11 PS.seek 2ml @22:02:57.85 Stroke completed -- waiting for pressure to equalize ... @22:02:57.86 SS.reconfigure :exhale @22:02:57.91 SS.hold @22:03:13.31 PTV.dial :puck @22:03:14.06 PSV.dial PTV @22:03:14.82 PBV.dial :waste1 @22:03:15.26 PS.seek 1.5ml @22:03:16.39 Intake.select :close @22:03:16.55 SSV.dial :exhaust @22:03:17.32 Exhaust.select :open @22:03:17.44 SS.seek 0ml @22:03:21.25 delay 3 seconds @22:03:24.27 PBV.dial between PSV and waste1 @22:03:24.64 PS.seek 0ml @22:03:26.48 Exhaust.select :close @22:03:26.59 SSV.dial CBV @22:03:26.63 CBV.dial SSV @22:03:27.40 Allowing 130s for 25.0ml of remaining 875.0ml @22:03:27.41 Intake.select :open @22:03:29.58 Intake @15.9psia, vacuum from 10.0 to 13.0psi @22:03:29.60 SS.reconfigure :inhaling 0.91ml/s @22:03:29.65 SS.seek 25ml @22:03:40.75 delay 2:00 @22:03:59.28 Stroke completed -- waiting for pressure to equalize ... @22:03:59.30 SS.reconfigure :exhale @22:03:59.34 SS.hold @22:04:16.12 Intake.select :close @22:04:16.28 SSV.dial :exhaust @22:04:17.05 Exhaust.select :open @22:04:17.17 SS.seek 0ml @22:04:26.16 Exhaust.select :close @22:04:26.27 SSV.dial CBV @22:04:26.31 CBV.dial SSV @22:04:27.07 Allowing 130s for 25.0ml of remaining 850.0ml @22:04:27.08 Intake.select :open @22:04:29.25 Intake @15.8psia, vacuum from 10.0 to 13.0psi @22:04:29.27 SS.reconfigure :inhaling 0.91ml/s @22:04:29.32 SS.seek 25ml @22:04:58.75 Stroke completed -- waiting for pressure to equalize ... @22:04:58.76 SS.reconfigure :exhale @22:04:58.81 SS.hold @22:05:15.27 Intake.select :close @22:05:15.42 SSV.dial :exhaust @22:05:16.19 Exhaust.select :open @22:05:16.31 SS.seek 0ml @22:05:25.31 Exhaust.select :close @22:05:25.42 SSV.dial CBV @22:05:25.46 CBV.dial SSV @22:05:26.22 Allowing 130s for 25.0ml of remaining 825.0ml @22:05:26.24 Intake.select :open @22:05:28.41 Intake @15.9psia, vacuum from 10.0 to 13.0psi @22:05:28.43 SS.reconfigure :inhaling 0.91ml/s @22:05:28.48 SS.seek 25ml @22:05:40.78 recoverPSRreagent08 @22:05:40.79 PTV.dial :puck @22:05:40.85 PBV.dial :waste1 @22:05:41.23 delay 30 seconds @22:05:57.69 Stroke completed -- waiting for pressure to equalize ... @22:05:57.70 SS.reconfigure :exhale @22:05:57.75 SS.hold @22:06:11.25 PBV.dial between PSV and waste1 @22:06:11.62 PSR.select :air @22:06:11.64 PSV.dial PSR @22:06:12.41 PS.reconfigure :fast @22:06:12.46 PS.seek 2.5ml @22:06:16.59 Intake.select :close @22:06:16.74 SSV.dial :exhaust @22:06:17.52 Exhaust.select :open @22:06:17.64 SS.seek 0ml @22:06:26.44 Exhaust.select :close @22:06:26.55 SSV.dial CBV @22:06:26.57 CBV.dial SSV @22:06:27.35 Allowing 130s for 25.0ml of remaining 800.0ml @22:06:27.36 Intake.select :open @22:06:29.53 Intake @15.7psia, vacuum from 10.0 to 13.0psi @22:06:29.55 SS.reconfigure :inhaling 0.91ml/s @22:06:29.59 SS.seek 25ml @22:06:36.62 PSV.dial PTV @22:06:37.39 delay 5 seconds @22:06:42.41 PSV.dial PSR @22:06:43.17 delay 5 seconds @22:06:48.19 PSV.dial PTV @22:06:49.03 delay 5 seconds @22:06:54.05 PSV.dial :air @22:06:54.83 PS.seek 0ml @22:06:58.92 Vacuum too high -- waiting up to 40 seconds @22:06:58.93 SS.reconfigure :exhale @22:06:58.99 SS.hold @22:07:08.96 Intake @16.7psia, vacuum from 10.0 to 13.0psi @22:07:08.97 SS.reconfigure :inhaling 0.73ml/s @22:07:09.02 SS.seek 25ml @22:07:09.90 Stroke completed -- waiting for pressure to equalize ... @22:07:09.92 SS.reconfigure :exhale @22:07:09.97 SS.hold @22:07:18.37 washCycleDA #2 of 2 @22:07:18.38 PSR.select :air @22:07:18.40 PSV.dial PSR @22:07:18.69 Intake.select :close @22:07:18.84 SSV.dial :exhaust @22:07:19.63 Exhaust.select :open @22:07:19.77 Pulling 0.5ml of PSR.wash @22:07:19.79 SS.seek 0ml @22:07:19.81 PSR.select :wash @22:07:19.97 PS.reconfigure :default @22:07:20.03 PS.seek 0.5ml @22:07:25.96 delay 5 seconds @22:07:28.55 Exhaust.select :close @22:07:28.66 SSV.dial CBV @22:07:28.68 CBV.dial SSV @22:07:29.45 Allowing 130s for 25.0ml of remaining 775.0ml @22:07:29.47 Intake.select :open @22:07:30.98 PSR.select :air @22:07:31.10 PS.seek 2ml @22:07:31.64 Intake @15.9psia, vacuum from 10.0 to 13.0psi @22:07:31.65 SS.reconfigure :inhaling 0.73ml/s @22:07:31.70 SS.seek 25ml @22:07:47.27 PTV.dial :puck @22:07:47.35 PSV.dial PTV @22:07:48.10 PBV.dial :waste1 @22:07:48.54 PS.seek 1.5ml @22:07:54.52 delay 3 seconds @22:07:57.54 PBV.dial between PSV and waste1 @22:07:57.92 PS.seek 0ml @22:08:08.04 Stroke completed -- waiting for pressure to equalize ... @22:08:08.06 SS.reconfigure :exhale @22:08:08.10 SS.hold @22:08:14.27 delay 2:00 @22:08:22.96 Intake.select :close @22:08:23.12 SSV.dial :exhaust @22:08:23.89 Exhaust.select :open @22:08:24.01 SS.seek 0ml @22:08:32.80 Exhaust.select :close @22:08:32.91 SSV.dial CBV @22:08:32.93 CBV.dial SSV @22:08:33.72 Allowing 130s for 25.0ml of remaining 750.0ml @22:08:33.73 Intake.select :open @22:08:35.90 Intake @15.8psia, vacuum from 10.0 to 13.0psi @22:08:35.92 SS.reconfigure :inhaling 0.73ml/s @22:08:35.97 SS.seek 25ml @22:09:12.40 Stroke completed -- waiting for pressure to equalize ... @22:09:12.41 SS.reconfigure :exhale @22:09:12.46 SS.hold @22:09:29.11 Intake.select :close @22:09:29.27 SSV.dial :exhaust @22:09:30.03 Exhaust.select :open @22:09:30.15 SS.seek 0ml @22:09:39.00 Exhaust.select :close @22:09:39.11 SSV.dial CBV @22:09:39.14 CBV.dial SSV @22:09:39.91 Allowing 130s for 25.0ml of remaining 725.0ml @22:09:39.92 Intake.select :open @22:09:42.09 Intake @15.8psia, vacuum from 10.0 to 13.0psi @22:09:42.11 SS.reconfigure :inhaling 0.73ml/s @22:09:42.16 SS.seek 25ml @22:10:14.30 recoverPSRreagent08 @22:10:14.31 PTV.dial :puck @22:10:14.38 PBV.dial :waste1 @22:10:15.21 delay 30 seconds @22:10:18.54 Stroke completed -- waiting for pressure to equalize ... @22:10:18.56 SS.reconfigure :exhale @22:10:18.60 SS.hold @22:10:34.93 Intake.select :close @22:10:35.09 SSV.dial :exhaust @22:10:35.86 Exhaust.select :open @22:10:35.97 SS.seek 0ml @22:10:45.03 Exhaust.select :close @22:10:45.14 SSV.dial CBV @22:10:45.17 CBV.dial SSV @22:10:45.23 PBV.dial between PSV and waste1 @22:10:46.00 PSR.select :air @22:10:46.09 PSV.dial PSR @22:10:46.15 Allowing 130s for 25.0ml of remaining 700.0ml @22:10:46.16 Intake.select :open @22:10:46.86 PS.reconfigure :fast @22:10:46.91 PS.seek 2.5ml @22:10:48.33 Intake @15.7psia, vacuum from 10.0 to 13.0psi @22:10:48.35 SS.reconfigure :inhaling 0.73ml/s @22:10:48.40 SS.seek 25ml @22:11:11.24 PSV.dial PTV @22:11:12.01 delay 5 seconds @22:11:17.03 PSV.dial PSR @22:11:17.79 delay 5 seconds @22:11:22.81 PSV.dial PTV @22:11:23.60 delay 5 seconds @22:11:24.29 Stroke completed -- waiting for pressure to equalize ... @22:11:24.30 SS.reconfigure :exhale @22:11:24.35 SS.hold @22:11:28.62 PSV.dial :air @22:11:29.38 PS.seek 0ml @22:11:45.23 Intake.select :close @22:11:45.39 SSV.dial :exhaust @22:11:46.15 Exhaust.select :open @22:11:46.27 SS.seek 0ml @22:11:52.96 getPMLReagent08 @22:11:52.97 PRV.dial :air @22:11:53.44 delay 5 seconds @22:11:55.28 Exhaust.select :close @22:11:55.39 SSV.dial CBV @22:11:55.42 CBV.dial SSV @22:11:56.18 Allowing 130s for 25.0ml of remaining 675.0ml @22:11:56.20 Intake.select :open @22:11:58.38 Intake @15.7psia, vacuum from 10.0 to 13.0psi @22:11:58.39 SS.reconfigure :inhaling 0.73ml/s @22:11:58.45 SS.seek 25ml @22:11:58.49 PTV.dial :PRVmixing @22:11:59.83 delay 5 seconds @22:12:04.85 PRV.dial :ab2 @22:12:05.32 PTV.dial :PRVmixing @22:12:05.39 PSV.dial :mixloop @22:12:05.88 PS.reconfigure :default @22:12:05.93 PS.seek 0.3ml @22:12:09.77 delay 2 seconds @22:12:11.79 PRV.dial :air @22:12:12.33 PS.seek 0.55ml @22:12:15.68 addPMLReagent08 @22:12:15.69 PSV.dial :air @22:12:16.29 PS.seek 2ml @22:12:31.92 PSV.dial :PML @22:12:32.38 PTV.dial :mixing @22:12:33.14 PBV.dial :waste1 @22:12:33.58 PS.seek 1.85ml @22:12:34.27 Stroke completed -- waiting for pressure to equalize ... @22:12:34.29 SS.reconfigure :exhale @22:12:34.33 SS.hold @22:12:35.97 PBV.dial between PSV and waste1 @22:12:36.82 PS.seek 0ml @22:12:56.58 delay 2 seconds @22:12:57.07 Intake.select :close @22:12:57.23 SSV.dial :exhaust @22:12:58.02 Exhaust.select :open @22:12:58.13 SS.seek 0ml @22:12:58.60 delay 8:00 @22:13:06.98 Exhaust.select :close @22:13:07.09 SSV.dial CBV @22:13:07.11 CBV.dial SSV @22:13:07.89 Allowing 130s for 25.0ml of remaining 650.0ml @22:13:07.90 Intake.select :open @22:13:10.07 Intake @15.7psia, vacuum from 10.0 to 13.0psi @22:13:10.09 SS.reconfigure :inhaling 0.73ml/s @22:13:10.14 SS.seek 25ml @22:13:46.44 Vacuum too high -- waiting up to 40 seconds @22:13:46.46 SS.reconfigure :exhale @22:13:46.91 SS.hold @22:13:58.57 Intake @15.3psia, vacuum from 9.8 to 12.8psi @22:13:58.58 SS.reconfigure :inhaling 0.59ml/s @22:13:58.63 SS.seek 25ml @22:13:58.88 Stroke completed -- waiting for pressure to equalize ... @22:13:58.89 SS.reconfigure :exhale @22:13:58.94 SS.hold @22:14:10.21 Intake.select :close @22:14:10.37 SSV.dial :exhaust @22:14:11.13 Exhaust.select :open @22:14:11.25 SS.seek 0ml @22:14:20.09 Exhaust.select :close @22:14:20.20 SSV.dial CBV @22:14:20.22 CBV.dial SSV @22:14:20.99 Allowing 130s for 25.0ml of remaining 625.0ml @22:14:21.01 Intake.select :open @22:14:23.17 Intake @15.8psia, vacuum from 10.0 to 13.0psi @22:14:23.19 SS.reconfigure :inhaling 0.59ml/s @22:14:23.24 SS.seek 25ml @22:15:08.43 Stroke completed -- waiting for pressure to equalize ... @22:15:08.44 SS.reconfigure :exhale @22:15:08.49 SS.hold @22:15:29.36 Intake.select :close @22:15:29.52 SSV.dial :exhaust @22:15:30.29 Exhaust.select :open @22:15:30.41 SS.seek 0ml @22:15:39.16 Exhaust.select :close @22:15:39.28 SSV.dial CBV @22:15:39.30 CBV.dial SSV @22:15:40.09 Allowing 130s for 25.0ml of remaining 600.0ml @22:15:40.10 Intake.select :open @22:15:42.27 Intake @15.7psia, vacuum from 10.0 to 13.0psi @22:15:42.29 SS.reconfigure :inhaling 0.59ml/s @22:15:42.34 SS.seek 25ml @22:16:27.33 Stroke completed -- waiting for pressure to equalize ... @22:16:27.34 SS.reconfigure :exhale @22:16:27.39 SS.hold @22:16:54.13 Intake.select :close @22:16:54.29 SSV.dial :exhaust @22:16:55.07 Exhaust.select :open @22:16:55.19 SS.seek 0ml @22:17:04.18 Exhaust.select :close @22:17:04.29 SSV.dial CBV @22:17:04.31 CBV.dial SSV @22:17:05.08 Allowing 130s for 25.0ml of remaining 575.0ml @22:17:05.10 Intake.select :open @22:17:07.26 Intake @15.7psia, vacuum from 10.0 to 13.0psi @22:17:07.28 SS.reconfigure :inhaling 0.59ml/s @22:17:07.33 SS.seek 25ml @22:17:51.53 Stroke completed -- waiting for pressure to equalize ... @22:17:51.94 SS.reconfigure :exhale @22:17:52.06 SS.hold @22:18:23.18 Intake.select :close @22:18:23.34 SSV.dial :exhaust @22:18:24.11 Exhaust.select :open @22:18:24.23 SS.seek 0ml @22:18:33.21 Exhaust.select :close @22:18:33.32 SSV.dial CBV @22:18:33.34 CBV.dial SSV @22:18:34.13 Allowing 130s for 25.0ml of remaining 550.0ml @22:18:34.15 Intake.select :open @22:18:36.31 Intake @15.7psia, vacuum from 10.0 to 13.0psi @22:18:36.33 SS.reconfigure :inhaling 0.59ml/s @22:18:36.38 SS.seek 25ml @22:19:14.49 Vacuum too high -- waiting up to 40 seconds @22:19:14.51 SS.reconfigure :exhale @22:19:14.56 SS.hold @22:19:33.13 Intake @16.8psia, vacuum from 10.0 to 13.0psi @22:19:33.15 SS.reconfigure :inhaling 0.47ml/s @22:19:33.19 SS.seek 25ml @22:19:42.02 Stroke completed -- waiting for pressure to equalize ... @22:19:42.03 SS.reconfigure :exhale @22:19:42.08 SS.hold @22:20:00.45 Intake.select :close @22:20:01.08 SSV.dial :exhaust @22:20:01.86 Exhaust.select :open @22:20:01.98 SS.seek 0ml @22:20:10.80 Exhaust.select :close @22:20:10.91 SSV.dial CBV @22:20:10.93 CBV.dial SSV @22:20:11.71 Allowing 130s for 25.0ml of remaining 525.0ml @22:20:11.72 Intake.select :open @22:20:13.89 Intake @15.8psia, vacuum from 10.0 to 13.0psi @22:20:13.91 SS.reconfigure :inhaling 0.47ml/s @22:20:13.96 SS.seek 25ml @22:20:58.62 recoverPRVreagent08 @22:20:58.63 PTV.dial :mixing @22:20:58.70 PBV.dial :waste1 @22:20:59.07 delay 30 seconds @22:21:09.45 Stroke completed -- waiting for pressure to equalize ... @22:21:09.47 SS.reconfigure :exhale @22:21:09.51 SS.hold @22:21:29.09 PBV.dial between PSV and waste1 @22:21:29.46 PSV.dial :air @22:21:30.06 PS.reconfigure :fast @22:21:30.11 PS.seek 2.5ml @22:21:44.26 Intake.select :close @22:21:44.42 SSV.dial :exhaust @22:21:45.20 Exhaust.select :open @22:21:45.32 SS.seek 0ml @22:21:54.32 PSV.dial :mixloop @22:21:54.40 Exhaust.select :close @22:21:54.52 SSV.dial CBV @22:21:54.54 CBV.dial SSV @22:21:54.78 delay 2 seconds @22:21:55.31 Allowing 130s for 25.0ml of remaining 500.0ml @22:21:55.33 Intake.select :open @22:21:56.79 PSV.dial :air @22:21:57.26 delay 2 seconds @22:21:57.49 Intake @15.7psia, vacuum from 10.0 to 13.0psi @22:21:57.51 SS.reconfigure :inhaling 0.47ml/s @22:21:57.56 SS.seek 25ml @22:21:59.28 PSV.dial :mixloop @22:21:59.76 delay 2 seconds @22:22:01.78 PSV.dial :air @22:22:02.25 PS.seek 0ml @22:22:25.73 washCycleDA #1 of 3 @22:22:25.74 PSR.select :air @22:22:25.75 PSV.dial PSR @22:22:27.10 Pulling 0.5ml of PSR.wash @22:22:27.11 PSR.select :wash @22:22:27.23 PS.reconfigure :default @22:22:27.28 PS.seek 0.5ml @22:22:33.23 delay 5 seconds @22:22:38.25 PSR.select :air @22:22:38.37 PS.seek 2ml @22:22:52.42 Stroke completed -- waiting for pressure to equalize ... @22:22:52.43 SS.reconfigure :exhale @22:22:52.48 SS.hold @22:22:55.00 PTV.dial :puck @22:22:55.76 PSV.dial PTV @22:22:56.52 PBV.dial :waste1 @22:22:56.96 PS.seek 1.5ml @22:23:02.97 delay 3 seconds @22:23:05.99 PBV.dial between PSV and waste1 @22:23:06.37 PS.seek 0ml @22:23:22.54 delay 2:00 @22:23:32.67 SS.reconfigure :default @22:23:32.72 SS.hold @22:23:32.79 Intake.select :close @22:23:32.96 ShallowSampler::Clogged in WCR -- Intake @15psia & Outlet @14psia won't equalize after 525.0ml @22:23:32.97 SSV.dial :exhaust @22:23:33.75 Exhaust.select :open @22:23:33.86 SS.reconfigure :exhale @22:23:33.92 SS.seek 0ml @22:23:42.75 Exhaust.select :close @22:23:42.86 Sampled 525.0ml @22:23:42.88 Drying puck before fixing for 5:00 @22:23:42.89 CBV.dial :waste2b @22:23:43.65 delay 1:00 @22:24:43.68 CSV.dial :air @22:24:44.13 CS.reconfigure :fast @22:24:44.18 CS.seek 10ml @22:25:08.89 Pumping wcr puck from tube 3 up to 35psi... @22:25:08.91 CBV.dial SSV @22:25:10.17 CBV.dial between CSV and waste2b @22:25:11.15 CSV.dial between air and CTV @22:25:11.47 CS.seek 7.272ml @22:25:18.67 CTV.dial CSV,avoiding: :air @22:25:18.69 CSV.dial CTV @22:25:19.53 CS.reconfigure :pressurize @22:25:19.58 CS.seek 0ml @22:25:22.56 recoverPSRreagent08 @22:25:22.57 PTV.dial :puck @22:25:22.65 PBV.dial :waste1 @22:25:23.02 delay 30 seconds @22:25:35.78 CBV.dial :waste2b @22:25:36.14 CS.reconfigure :default @22:25:36.19 CS.seek 0ml @22:25:40.32 delay 1:00 @22:25:53.04 PBV.dial between PSV and waste1 @22:25:53.41 PSR.select :air @22:25:53.43 PSV.dial PSR @22:25:54.21 PS.reconfigure :fast @22:25:54.26 PS.seek 2.5ml @22:26:18.38 PSV.dial PTV @22:26:19.27 delay 5 seconds @22:26:24.29 PSV.dial PSR @22:26:25.04 delay 5 seconds @22:26:30.06 PSV.dial PTV @22:26:30.82 delay 5 seconds @22:26:35.84 PSV.dial :air @22:26:36.62 PS.seek 0ml @22:26:40.34 CTV.dial :air,avoiding: :intake @22:26:41.69 Fix #1 of 2 (with RNAlater) for 2:30 @22:26:41.71 CSV.dial CSR @22:26:42.15 Pulling 2ml of CSR.RNAlater @22:26:42.16 CSR.select :RNAlater @22:26:42.28 CS.seek 2ml @22:26:48.17 delay 5 seconds @22:26:53.19 CSR.select :air @22:26:53.31 CS.reconfigure :fast @22:26:53.36 CS.seek 10ml @22:27:00.38 washCycleDA #2 of 3 @22:27:00.39 PSR.select :air @22:27:00.41 PSV.dial PSR @22:27:01.75 Pulling 0.5ml of PSR.wash @22:27:01.76 PSR.select :wash @22:27:01.88 PS.reconfigure :default @22:27:01.93 PS.seek 0.5ml @22:27:07.91 delay 5 seconds @22:27:12.93 PSR.select :air @22:27:13.05 PS.seek 2ml @22:27:13.23 Pumping wcr puck from tube 3 up to 35psi... @22:27:13.25 CBV.dial SSV @22:27:14.52 CBV.dial between CSV and waste2b @22:27:15.49 CSV.dial between air and CTV @22:27:16.22 CS.seek 7.206ml @22:27:23.45 CTV.dial CSV,avoiding: :air @22:27:23.48 CSV.dial CTV @22:27:24.89 CS.reconfigure :pressurize @22:27:24.94 CS.seek 0ml @22:27:29.25 PTV.dial :puck @22:27:29.33 PSV.dial PTV @22:27:30.08 PBV.dial :waste1 @22:27:30.52 PS.seek 1.5ml @22:27:36.44 delay 3 seconds @22:27:39.46 PBV.dial between PSV and waste1 @22:27:39.84 PS.seek 0ml @22:27:43.69 delay 2:30 @22:27:56.00 delay 2:00 @22:29:56.03 recoverPSRreagent08 @22:29:56.04 PTV.dial :puck @22:29:56.11 PBV.dial :waste1 @22:29:56.49 delay 30 seconds @22:30:13.71 CBV.dial :waste2b @22:30:14.04 CS.reconfigure :default @22:30:14.09 CS.seek 0ml @22:30:15.35 delay 1:00 @22:30:26.51 PBV.dial between PSV and waste1 @22:30:26.89 PSR.select :air @22:30:26.91 PSV.dial PSR @22:30:27.80 PS.reconfigure :fast @22:30:27.85 PS.seek 2.5ml @22:30:52.06 PSV.dial PTV @22:30:52.81 delay 5 seconds @22:30:57.83 PSV.dial PSR @22:30:58.61 delay 5 seconds @22:31:03.63 PSV.dial PTV @22:31:04.41 delay 5 seconds @22:31:09.43 PSV.dial :air @22:31:10.19 PS.seek 0ml @22:31:15.37 CTV.dial :air,avoiding: :intake @22:31:16.71 Fix #2 of 2 (with RNAlater) for 2:30 @22:31:16.73 CSV.dial CSR @22:31:17.18 Pulling 2ml of CSR.RNAlater @22:31:17.19 CSR.select :RNAlater @22:31:17.31 CS.seek 2ml @22:31:23.35 delay 5 seconds @22:31:28.37 CSR.select :air @22:31:28.49 CS.reconfigure :fast @22:31:28.54 CS.seek 10ml @22:31:33.76 washCycleDA #3 of 3 @22:31:33.77 PSR.select :air @22:31:33.78 PSV.dial PSR @22:31:35.12 Pulling 0.5ml of PSR.wash @22:31:35.13 PSR.select :wash @22:31:35.25 PS.reconfigure :default @22:31:35.30 PS.seek 0.5ml @22:31:41.31 delay 5 seconds @22:31:46.33 PSR.select :air @22:31:46.45 PS.seek 2ml @22:31:48.35 Pumping wcr puck from tube 3 up to 35psi... @22:31:48.37 CBV.dial SSV @22:31:49.64 CBV.dial between CSV and waste2b @22:31:50.61 CSV.dial between air and CTV @22:31:51.72 CS.seek 7.133ml @22:31:59.14 CTV.dial CSV,avoiding: :air @22:31:59.16 CSV.dial CTV @22:32:00.54 CS.reconfigure :pressurize @22:32:00.60 CS.seek 0ml @22:32:02.56 PTV.dial :puck @22:32:02.64 PSV.dial PTV @22:32:03.39 PBV.dial :waste1 @22:32:03.82 PS.seek 1.5ml @22:32:09.84 delay 3 seconds @22:32:12.86 PBV.dial between PSV and waste1 @22:32:13.24 PS.seek 0ml @22:32:16.87 delay 2:30 @22:32:29.40 delay 2:00 @22:34:29.43 recoverPSRreagent08 @22:34:29.44 PTV.dial :puck @22:34:29.51 PBV.dial :waste1 @22:34:29.89 delay 30 seconds @22:34:46.90 CBV.dial :waste2b @22:34:47.24 CS.reconfigure :default @22:34:47.29 CS.seek 0ml @22:34:50.69 delay 1:00 @22:34:59.91 PBV.dial between PSV and waste1 @22:35:00.29 PSR.select :air @22:35:00.31 PSV.dial PSR @22:35:01.08 PS.reconfigure :fast @22:35:01.13 PS.seek 2.5ml @22:35:25.18 PSV.dial PTV @22:35:25.95 delay 5 seconds @22:35:30.97 PSV.dial PSR @22:35:31.71 delay 5 seconds @22:35:36.73 PSV.dial PTV @22:35:37.51 delay 5 seconds @22:35:42.53 PSV.dial :air @22:35:43.30 PS.seek 0ml @22:35:50.71 CTV.dial :air,avoiding: :intake @22:36:06.90 Gate.power :camera,:ON @22:36:06.91 Mixing DA Substrates @22:36:06.92 PSR.select :air @22:36:06.94 PSV.dial PSR @22:36:08.34 Pulling 0.25ml of PSR.sub1 @22:36:08.37 PSR.select :sub1 @22:36:08.57 PS.reconfigure :default @22:36:08.68 PS.seek 0.25ml @22:36:12.03 delay 5 seconds @22:36:17.05 Pulling 0.25ml of PSR.sub2 @22:36:17.07 PSR.select :sub2 @22:36:17.18 PS.seek 0.5ml @22:36:20.52 delay 5 seconds @22:36:25.54 PSR.select :air @22:36:25.66 PS.seek 2ml @22:36:41.80 PTV.dial :puck @22:36:41.88 PSV.dial PTV @22:36:42.66 PBV.dial :waste1 @22:36:43.58 PS.seek 1.5ml @22:36:49.49 delay 3 seconds @22:36:52.51 PBV.dial between PSV and waste1 @22:36:52.89 PS.seek 0ml @22:37:09.02 delay 10 seconds @22:37:19.05 recoverPSRreagent08 @22:37:19.06 PTV.dial :puck @22:37:19.13 PBV.dial :waste1 @22:37:19.51 delay 30 seconds @22:37:49.54 PBV.dial between PSV and waste1 @22:37:49.91 PSR.select :air @22:37:49.93 PSV.dial PSR @22:37:50.72 PS.reconfigure :fast @22:37:50.77 PS.seek 2.5ml @22:38:15.18 PSV.dial PTV @22:38:16.07 delay 5 seconds @22:38:21.09 PSV.dial PSR @22:38:21.84 delay 5 seconds @22:38:26.86 PSV.dial PTV @22:38:27.63 delay 5 seconds @22:38:32.65 PSV.dial :air @22:38:33.45 PS.seek 0ml @22:38:57.59 Additional air clear @22:38:57.60 PSR.select :air @22:38:57.61 PSV.dial PSR @22:38:58.97 Pulling 2ml of PSR.air @22:38:58.98 PSR.select :air @22:38:59.01 PS.reconfigure :default @22:38:59.05 PS.seek 2ml @22:39:20.36 delay 5 seconds @22:39:25.39 PTV.dial :puck @22:39:25.47 PSV.dial PTV @22:39:26.25 PBV.dial :waste1 @22:39:26.69 PS.seek 1.5ml @22:39:32.93 delay 3 seconds @22:39:35.95 PBV.dial between PSV and waste1 @22:39:36.32 delay 1 second @22:39:37.34 PSV.dial :waste1 @22:39:38.43 PS.seek 0ml @22:39:54.70 Processing Puck Heater at 27.9C before imaging @22:39:54.71 PH.coast @22:39:54.81 Imaging da puck in Processing Clamp while storing it to tube 2 @22:39:54.99 SC.reconfigure :tight @22:39:55.04 SC.seek 2 @22:39:58.58 SC.configure :limp @22:39:58.79 Elbow.reconfigure :tight @22:39:58.84 Elbow.seek 2 @22:40:00.65 Forearm.reconfigure :tight @22:40:00.70 Forearm.seek 2 + 450 counts @22:40:01.72 SE.reconfigure :pick @22:40:01.78 SE.seek :beamBreak @22:40:03.77 SE.configure :default @22:40:04.00 Elbow.reconfigure :limp @22:40:04.05 Forearm.reconfigure :loose @22:40:04.10 Forearm.seek 2 @22:40:04.35 Elbow.hold @22:40:04.42 Elbow.reconfigure :tight @22:40:04.52 SE.seek 24.09 Pucks below Top Plate @22:40:04.97 Forearm.force -20 @22:40:05.02 Forearm.seek 2 + 309 counts @22:40:05.32 Forearm.reconfigure :tight @22:40:05.37 Forearm.seek 2 + 450 counts @22:40:05.59 SE.seek 25.09 Pucks below Top Plate @22:40:06.14 Forearm.reconfigure :loose @22:40:06.18 Forearm.seek :retract @22:40:07.16 Elbow.seek PC @22:40:09.72 Elbow.reconfigure :loose @22:40:09.82 Forearm.seek PC @22:40:10.79 Hand.close @22:40:11.41 PC.seek :open @22:40:16.19 Forearm.seek :retract @22:40:17.11 Elbow.reconfigure :tight @22:40:17.16 Elbow.seek 2 @22:40:19.76 Forearm.reconfigure :tight @22:40:19.81 Forearm.seek 2 + 450 counts @22:40:20.78 Hand.open @22:40:21.48 SE.seek 24.74 Pucks below Top Plate @22:40:21.77 SE.reconfigure :seal @22:40:21.82 SE.seek 23.77 Pucks below Top Plate @22:40:22.35 Lights, camera, ACTION! @22:40:22.38 PuckCamera: snap -bin=2,2 -auto /var/log/esp/da23nov1322h700ml.tif @22:40:41.87 Waiting >20 seconds for da to relinquish FlushPuck @22:40:51.58 SX HX9/16 (USB:02): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 25.675 seconds /var/log/esp/da23nov1322h700ml.tif: TIFF Upload Complete @22:40:51.61 PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da23nov1322h700ml15s.tif @22:41:07.23 SX HX9/16 (USB:02): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15 seconds /var/log/esp/midres/da23nov1322h700ml15s.tif: TIFF Upload Complete @22:41:07.25 PuckCamera: snap -bin=2,2 20.0 /var/log/esp/midres/da23nov1322h700ml20s.tif @22:41:27.91 SX HX9/16 (USB:02): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 20 seconds /var/log/esp/midres/da23nov1322h700ml20s.tif: TIFF Upload Complete @22:41:27.93 DAfans.email "25.675 sec auto-exposure",Subject: "da23nov1322h700ml images!" @22:41:27.96 Gate.power :camera,:OFF @22:41:28.90 Elbow.seek 2 @22:41:29.13 Forearm.seek 2 + 450 counts @22:41:29.21 SE.reconfigure :gently @22:41:29.28 SE.seek 24.49 Pucks below Top Plate @22:41:29.97 Elbow.reconfigure :limp @22:41:30.03 Forearm.reconfigure :loose @22:41:30.08 Forearm.seek 2 @22:41:30.31 Elbow.hold @22:41:30.38 Elbow.reconfigure :tight @22:41:30.45 SE.reconfigure :default @22:41:30.51 SE.seek 24.67 Pucks below Top Plate @22:41:30.99 Forearm.force -20 @22:41:31.03 Forearm.seek 2 + 315 counts @22:41:31.50 Forearm.reconfigure :tight @22:41:31.73 Forearm.seek 2 + 450 counts @22:41:32.05 SE.seek :down @22:41:33.17 SE.stop @22:41:33.47 Forearm.reconfigure :loose @22:41:33.58 Forearm.seek :retract @22:41:34.71 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @22:41:35.26 Elbow.seek FlushPuck::Garage @22:41:37.32 Elbow.reconfigure :loose @22:41:37.53 Forearm.seek :garage @22:41:38.59 Hand.close @22:41:39.55 Forearm.seek :retract @22:41:40.73 Elbow.reconfigure :tight @22:41:40.83 Elbow.seek PC @22:41:43.23 Elbow.reconfigure :loose @22:41:43.50 Forearm.reconfigure :tight @22:41:43.60 Forearm.seek PC @22:41:45.26 PC.seek :closed @22:41:50.08 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.60 @22:41:50.21 Hand.open @22:41:51.07 Forearm.reconfigure :loose @22:41:51.16 Forearm.seek :retract @22:41:52.15 PSR.select :air @22:41:52.21 Storing wcr puck in Collection Clamp to tube 2 @22:41:52.25 PSV.dial PSR @22:41:53.04 Pulling 0.5ml of PSR.flush @22:41:53.11 PSR.select :flush @22:41:53.50 Elbow.reconfigure :tight @22:41:53.59 Elbow.seek 2 @22:41:53.70 PS.seek 0.5ml @22:41:56.52 Forearm.reconfigure :tight @22:41:56.62 Forearm.seek 2 + 450 counts @22:41:57.80 SE.reconfigure :pick @22:41:57.91 SE.seek :beamBreak @22:41:59.49 SE.configure :default @22:41:59.82 delay 5 seconds @22:42:00.04 Elbow.reconfigure :limp @22:42:00.13 Forearm.reconfigure :loose @22:42:01.24 Forearm.seek 2 @22:42:01.53 Elbow.hold @22:42:01.66 Elbow.reconfigure :tight @22:42:01.88 SE.seek 25.09 Pucks below Top Plate @22:42:02.58 Forearm.force -20 @22:42:02.68 Forearm.seek 2 + 294 counts @22:42:03.16 Forearm.reconfigure :tight @22:42:03.26 Forearm.seek 2 + 450 counts @22:42:03.54 SE.seek 26.09 Pucks below Top Plate @22:42:04.31 Forearm.reconfigure :loose @22:42:04.40 Forearm.seek :retract @22:42:04.86 PSR.select :air @22:42:04.98 PS.seek 2.5ml @22:42:05.15 upsync @22:42:05.36 Elbow.seek CC @22:42:06.98 Elbow.reconfigure :loose @22:42:07.09 Forearm.seek CC @22:42:08.13 Hand.close @22:42:08.79 CC.seek :open @22:42:13.58 Forearm.seek :retract @22:42:14.55 Elbow.reconfigure :tight @22:42:14.60 Elbow.seek 2 @22:42:16.39 Elbow.reconfigure :limp @22:42:16.44 Forearm.seek 2 @22:42:17.45 Elbow.hold @22:42:17.52 Elbow.reconfigure :tight @22:42:17.57 Hand.open @22:42:18.36 Forearm.force -20 @22:42:18.41 Forearm.seek 2 + 306 counts @22:42:18.72 Forearm.reconfigure :tight @22:42:18.77 Forearm.seek 2 + 450 counts @22:42:19.02 SE.seek :down @22:42:19.95 SE.stop @22:42:20.16 Forearm.reconfigure :loose @22:42:20.21 Forearm.seek :retract @22:42:21.13 Starting finalPurge @22:42:26.17 delay 1 second @22:42:27.19 PRV.dial :waste2,avoiding: :ab2 @22:42:29.06 PTV.dial :PRVmixing @22:42:30.39 PSV.dial :mixloop @22:42:31.47 PS.seek :empty @22:42:40.48
Waiting >20 seconds for psFlush to relinquish FlushPuck @22:42:57.86 PSR.select :air @22:42:57.88 PSV.dial PSR @22:42:58.94 Pulling 0.5ml of PSR.flush @22:42:58.95 PSR.select :flush @22:42:59.07 PS.seek 0.5ml @22:43:04.98 delay 5 seconds @22:43:10.01 PSR.select :air @22:43:10.13 PS.seek 2.5ml @22:43:31.36 delay 1 second @22:43:32.38 PBV.dial :waste1 @22:43:32.73 PTV.dial :mixing @22:43:33.48 PSV.dial :mixloop @22:43:34.55 PS.seek :empty @22:44:00.89 PSV.dial PSR @22:44:01.95 PSR.select :flush @22:44:02.08 PS.seek 1ml @22:44:13.08 PSR.select :air @22:44:13.20 PS.seek 2.5ml @22:44:29.29 PSV.dial PTV @22:44:30.06 PTV.dial :puck @22:44:30.81 PBV.dial :waste1 @22:44:30.90 PS.seek 0ml @22:44:57.20 PSV.dial PSR @22:44:57.95 PSR.select :flush @22:44:58.07 PS.seek 0.5ml @22:45:04.07 PSR.select :air @22:45:04.63 PS.seek 2.5ml @22:45:25.83 PSV.dial PTV @22:45:26.59 PBV.dial PSV @22:45:27.16 PS.seek 0ml @22:45:53.51 PSV.dial PBV @22:45:53.99 PS.seek 2.5ml @22:46:20.36 PSV.dial :waste1 @22:46:21.10 PS.seek 0ml @22:46:47.42 PSV.dial PSR @22:46:47.89 PSR.select :air @22:46:47.91 PS.seek 2.5ml @22:47:14.32 PSV.dial PTV @22:47:15.18 PBV.dial PSV @22:47:15.27 PS.seek 0ml @22:47:41.54 PSV.dial PBV @22:47:42.00 PS.seek 2.5ml @22:48:08.32 PSV.dial :waste1 @22:48:09.08 PS.seek 0ml @22:48:35.60 PSV.dial PSR @22:48:36.07 PSR.select :flush @22:48:36.20 PS.seek 1ml @22:48:47.20 PSR.select :air @22:48:47.32 PS.seek 2.5ml @22:49:03.63 PSV.dial PTV @22:49:04.49 PTV.dial :puck @22:49:04.57 PBV.dial :waste1 @22:49:05.14 PS.seek 0ml @22:49:31.45 PSV.dial PSR @22:49:32.20 PSR.select :flush @22:49:32.31 PS.seek 0.5ml @22:49:38.32 PSR.select :air @22:49:38.44 PS.seek 2.5ml @22:49:59.69 PSV.dial PTV @22:50:00.46 PBV.dial PSV @22:50:01.03 PS.seek 0ml @22:50:27.54 PSV.dial PBV @22:50:28.01 PS.seek 2.5ml @22:50:54.41 PSV.dial :waste1 @22:50:55.15 PS.seek 0ml @22:51:21.54 PSV.dial PSR @22:51:22.01 PSR.select :air @22:51:22.03 PS.seek 2.5ml @22:51:48.35 PSV.dial PTV @22:51:49.65 PBV.dial PSV @22:51:49.73 PS.seek 0ml @22:52:16.07 PSV.dial PBV @22:52:16.53 PS.seek 2.5ml @22:52:42.88 PSV.dial :waste1 @22:52:43.62 PS.seek 0ml @22:53:10.14
Loading FlushPuck into Collection Clamp from Processing Clamp @22:53:10.32 Elbow.seek PC @22:53:12.83 Elbow.reconfigure :loose @22:53:12.93 Forearm.seek PC @22:53:13.90 Hand.close @22:53:14.53 PC.seek :open @22:53:19.24 Forearm.seek :retract @22:53:20.26 Elbow.reconfigure :tight @22:53:20.31 Elbow.seek CC @22:53:23.94 Elbow.reconfigure :loose @22:53:24.04 Forearm.reconfigure :tight @22:53:24.08 Forearm.seek CC @22:53:25.31 CC.seek :closed @22:53:29.84 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.36 @22:53:29.90 Hand.open @22:53:30.96 Forearm.reconfigure :loose @22:53:31.01 Forearm.seek :retract @22:53:31.96 Flushing the Collection Valve::Manifold @22:53:32.09 CSV.dial CSR @22:53:32.52 Pulling 5ml of CSR.flush CSR.select :flush @22:53:33.09 CS.seek 5ml @22:53:46.67 delay 5 seconds @22:53:51.69 CSR.select :air @22:53:51.86 CS.reconfigure :fast @22:53:51.91 CS.seek 7.501ml @22:53:58.76 CSV.dial :waste @22:54:00.07 CS.reconfigure :default @22:54:00.11 CS.seek 0ml @22:54:20.14 Flushing lower manifold's waste1 port @22:54:20.27 CSV.dial :flush @22:54:20.71 CS.reconfigure :fast @22:54:20.76 CS.seek 5ml @22:54:33.55 delay 5 seconds @22:54:38.57 CSV.dial :air @22:54:39.09 CS.seek 7ml @22:54:44.70 CSV.dial CTV @22:54:44.73
CTV.dial CSV @22:54:46.09 CBV.dial :waste1 @22:54:46.88 CS.seek 0ml @22:55:03.67 Flushing lower manifold's waste2 port @22:55:03.79 CSV.dial :flush @22:55:04.52 CS.seek 5ml @22:55:17.30 delay 5 seconds @22:55:22.31 CSV.dial :air @22:55:22.84 CS.seek 7ml @22:55:28.39 CSV.dial CTV @22:55:28.41
CTV.dial CSV @22:55:28.86 CBV.dial :waste2 @22:55:29.91 CS.seek 0ml @22:55:46.70 Flushing lower manifold's waste2b port @22:55:46.82 CSV.dial :flush @22:55:47.57 CS.seek 5ml @22:56:00.29 delay 5 seconds @22:56:05.31 CSV.dial :air @22:56:05.82 CS.seek 7.001ml @22:56:11.36 CSV.dial CTV @22:56:11.38
CTV.dial CSV @22:56:11.95 CBV.dial :waste2b @22:56:13.02 CS.seek 0ml @22:56:29.80 Disinfecting external Intake with kill @22:56:29.91 CSV.dial CSR @22:56:30.48 Pulling 8ml of CSR.kill @22:56:30.49 CSR.select :kill @22:56:30.60 CS.seek 8ml @22:56:50.51 delay 5 seconds @22:56:55.53 CSR.select :air @22:56:55.65 CS.seek 10ml @22:57:01.61 CSV.dial CBV @22:57:01.63
CBV.dial CSV @22:57:02.73 CTV.dial :intake @22:57:03.51 Intake.select :open @22:57:03.63 CS.seek 2ml @22:57:22.64 Intake.select :close @22:57:22.74 CTV.dial :air @22:57:23.52 CS.reconfigure :default @22:57:23.56 CS.seek 0ml @22:57:29.54 CSV.dial CSR @22:57:30.68 Pulling 5ml of CSR.flush @22:57:30.69 CSR.select :flush @22:57:30.81 CS.seek 5ml @22:57:44.45 delay 5 seconds @22:57:49.47 CSR.select :air @22:57:49.59 CS.seek 10ml @22:58:03.17 CSV.dial CBV @22:58:04.17 CS.seek 0ml @22:58:30.64 Garaging FlushPuck in Collection Clamp @22:58:30.71 Elbow.reconfigure :tight @22:58:30.76 Elbow.seek CC @22:58:31.03 Elbow.reconfigure :loose @22:58:31.13 Forearm.seek CC @22:58:32.14 Hand.close @22:58:32.77 CC.seek :open @22:58:37.44 Forearm.seek :retract @22:58:38.41 Elbow.reconfigure :tight @22:58:38.46 Elbow.seek :garage @22:58:41.44 Elbow.reconfigure :loose @22:58:41.54 Forearm.reconfigure :tight @22:58:41.59 Forearm.seek :garage @22:58:42.65 Hand.open @22:58:43.33 Forearm.reconfigure :loose @22:58:43.38 Forearm.seek :retract @22:58:44.33 Completed [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] begun 20:28:45 @22:58:44.39 Asleep after completing DA_WCR_110223 assays @22:58:44.41 GoodNews.email "{1 => 33,\n 2 => 27,\n 3 => 6,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@22:57:55, 31% flow, 16.5C, 28% humidity, 15.2psia, 13.666V, 0.490A, 1.577Ah, 6.69W\nSampled 525.0ml",Subject: "Asleep after completing DA_WCR_110223 assays" @22:58:48.82 upsync + ESPwhich DA_WCR_11123.rb + fn=DA_WCR_11123.rb + IFS=: + set /home/esp/esp2/mission . + fullPath=/home/esp/esp2/mission/DA_WCR_11123.rb + [ -f /home/esp/esp2/mission/DA_WCR_11123.rb ] + fullPath=./DA_WCR_11123.rb + [ -f ./DA_WCR_11123.rb ] + return 1 + scriptPath= + [ DA_WCR_11123.rb = DA_WCR_11123 ] + echo No 'DA_WCR_11123.rb' script found on ESPpath: No 'DA_WCR_11123.rb' script found on ESPpath: + echo /home/esp/esp2/mission:. /home/esp/esp2/mission:. + exit 1 + ESPwhich DA_WCR_111523.rb + fn=DA_WCR_111523.rb + IFS=: + set /home/esp/esp2/mission . + fullPath=/home/esp/esp2/mission/DA_WCR_111523.rb + [ -f /home/esp/esp2/mission/DA_WCR_111523.rb ] + realpath /home/esp/esp2/mission/DA_WCR_111523.rb + return 0 + scriptPath=/home/esp/esp2local/mission/DA_WCR_111523.rb + exec ruby /home/esp/esp2/lib/esp.rb /home/esp/esp2local/mission/DA_WCR_111523.rb @12:41:19.56UTC15-Nov-23
ESP2 v3.42 I2C Gateway v3.20 for core bus @12:41:22.23 Configuration for short puck jake 1/11/21 by brent@mbari.org /home/esp/esp2/type/shallow/jake/configure.rb @12:41:26.02 Polling Can every 10:00 @12:41:26.07 Cleanly exited at 12:36:14UTC15-Nov-23 @12:41:26.12 Loading /home/esp/esp2local/mission/DA_WCR_111523.rb mission @12:41:26.18 GoodNews.email "Starting DA_WCR_111523 w/pucks in tube 3\n{1 => 33,\n 2 => 27,\n 3 => 6,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@12:41:26, 31% flow, 16.3C, 25% humidity, 15.2psia, 13.734V, 0.284A, 12.527Ah, 3.91W",Subject: "DA_WCR_111523 begun" @12:41:26.22
Preparing to sleep until + 00:00:05 @12:41:26.25 Asleep til + 00:00:05 @12:41:26.26 Continuing at 12:41 @12:41:26.55 Gate.power :core,:OFF @12:41:26.56 Gate.power :raw,:OFF @12:41:26.57 Shutdown @12:41:29.77 GoodNews.email "Then, in tube 3: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_111523.rb:14\n{1 => 33,\n 2 => 27,\n 3 => 6,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@12:41:26, 31% flow, 16.3C, 25% humidity, 15.2psia, 13.730V, 0.290A, 12.527Ah, 3.99W",Subject: "Asleep until 12:41" @12:41:31.17
Powering core bus @12:41:31.25 upsync @12:41:34.16 upsync @12:41:37.06
Gate.power :raw,:ON @12:41:37.72 CS.configure :default @12:41:37.77 PS.configure :default @12:41:37.82 SS.configure :default @12:41:37.86 CC.configure :default @12:41:37.91 PC.configure :default @12:41:37.98 Forearm.configure :loose @12:41:38.03 Elbow.configure :loose @12:41:38.10 SC.configure :loose @12:41:38.15 SE.configure :default @12:41:38.19 CH.configure :default @12:41:38.23 PH.configure :default @12:41:38.27 All dwarf controllers running firmware version 3.85 @12:41:38.38 Valve.reset @12:41:38.72 Intake.select :close @12:41:38.76 PSV.dial :air @12:41:38.78 Exhaust.select :close @12:41:38.81 CTV.dial :air @12:41:38.90
Forearm.home.seek :retract @12:41:38.96 CSV.dial :waste1 @12:41:39.42 SSV.dial :spit,avoiding: :exhaust @12:41:39.64 Hand.close @12:41:39.66
Elbow.reconfigure :loose @12:41:39.76 Elbow.jog 1500 @12:41:39.98 PS.home.seek 0ml @12:41:40.30 CS.reconfigure :fast @12:41:40.37 SS.home.seek 0ml @12:41:40.40 CS.home.seek 0ml @12:41:40.52
Elbow.reconfigure :loose @12:41:40.56 Elbow.home.seek :clear @12:41:45.08 SE.home.seek :down @12:41:45.70 SC.reconfigure :tight @12:41:45.75 SC.home.seek :initial @12:41:48.19 PS.stop @12:42:14.06
SC.configure :limp @12:42:14.12 Forearm.seek :retract @12:42:14.19 Elbow.seek :hammer @12:42:15.23 Forearm.reconfigure :tight @12:42:15.28 Forearm.seek retracted @12:42:15.54 PC.home.seek :open @12:42:15.55
CC.home.seek :open @12:42:19.33 Phase [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] @12:42:19.34 Notice.email "At 12:42:19 began in tube 3: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_111523.rb:14\n{1 => 33,\n 2 => 27,\n 3 => 6,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@12:42:19, 31% flow, 16.3C, 25% humidity, 15.2psia, 13.686V, 0.296A, 12.533Ah, 4.06W",Subject: "Started DA_WCR_111523 phase initialPurge; da [1000, 1000] #da and wsr are run simultanelously" @12:42:19.35
Starting initialPurge @12:42:19.38 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @12:42:19.44 Hand.open @12:42:20.24 Elbow.reconfigure :tight @12:42:20.30 Elbow.seek FlushPuck::Garage @12:42:22.19 Elbow.reconfigure :loose @12:42:22.31 Forearm.reconfigure :loose @12:42:22.42 Forearm.seek :garage @12:42:23.42 Hand.close @12:42:23.89 upsync @12:42:24.10
Forearm.seek :retract @12:42:25.13 Elbow.reconfigure :tight @12:42:25.18 Elbow.seek CC @12:42:28.15 Elbow.reconfigure :loose @12:42:28.25 Forearm.reconfigure :tight @12:42:28.30 Forearm.seek CC @12:42:29.60 CC.seek :closed @12:42:34.18 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.49 @12:42:34.25 Hand.open @12:42:34.92 Forearm.reconfigure :loose @12:42:34.96 Forearm.seek :retract @12:42:35.95 Flushing the Collection Valve::Manifold @12:42:36.07 CSV.dial CSR @12:42:37.37 Pulling 4.999ml of CSR.flush @12:42:37.38 CSR.select :flush @12:42:37.49 CS.reconfigure :default @12:42:37.95 CS.seek 5ml @12:42:51.58 delay 5 seconds @12:42:56.60 CSR.select :air @12:42:56.77 CS.reconfigure :fast @12:42:56.82 CS.seek 7.501ml @12:43:03.65 CSV.dial :waste @12:43:04.95 CS.reconfigure :default @12:43:05.00 CS.seek 0ml @12:43:25.09 Purging external Intake @12:43:25.21 CSV.dial :flush @12:43:25.65 CS.reconfigure :fast @12:43:25.70 CS.seek 10ml @12:43:50.15 delay 5 seconds @12:43:55.17 CSV.dial CBV @12:43:55.20
CBV.dial CSV @12:43:55.94 CTV.dial :intake @12:43:56.74 Intake.select :open @12:43:56.85 CS.seek 0ml @12:44:20.36 Intake.select :close @12:44:20.47 CTV.dial :air @12:44:21.26 Flushing upper manifold @12:44:21.38 CSV.dial :flush @12:44:22.12 CS.seek 5ml @12:44:34.86 delay 5 seconds @12:44:39.88 CSV.dial :air @12:44:40.39 CS.seek 7.001ml @12:44:45.93 CSV.dial CBV @12:44:45.95
CBV.dial CSV @12:44:46.99 CTV.dial :air @12:44:47.07 CS.seek 0ml @12:45:03.71 delay 7 seconds @12:45:10.74 CS.seek 3ml @12:45:18.71 CSV.dial :waste @12:45:19.17 CS.seek 0ml @12:45:26.94 CSV.dial :flush @12:45:27.39 CS.seek 3ml @12:45:35.46 delay 5 seconds @12:45:40.48 CSV.dial :air @12:45:40.97 CS.seek 5ml @12:45:46.71 CSV.dial :air @12:45:46.79 CS.seek 0ml @12:45:59.02 delay 7 seconds @12:46:06.05 CS.seek 3ml @12:46:13.95 CSV.dial :waste @12:46:14.70 CS.seek 0ml @12:46:22.43 Garaging FlushPuck in Collection Clamp @12:46:22.50 Elbow.reconfigure :tight @12:46:22.54 Elbow.seek CC @12:46:22.84 Elbow.reconfigure :loose @12:46:22.94 Forearm.seek CC @12:46:23.99 Hand.close @12:46:24.60 CC.seek :open @12:46:29.63 Forearm.seek :retract @12:46:30.63 Elbow.reconfigure :tight @12:46:30.68 Elbow.seek :garage @12:46:33.53 Elbow.reconfigure :loose @12:46:33.63 Forearm.reconfigure :tight @12:46:33.68 Forearm.seek :garage @12:46:34.65 Hand.open @12:46:35.28 Forearm.reconfigure :loose @12:46:35.33 Forearm.seek :retract @12:46:36.31 da sampling at most 1000ml, wcr at most 1000ml @12:46:36.35 DAfans.email "Started da in tube 3 at 12:46:36UTC15-Nov-23\n{1 => 33,\n 2 => 27,\n 3 => 6,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@12:42:19, 31% flow, 16.3C, 25% humidity, 15.2psia, 13.686V, 0.296A, 12.533Ah, 4.06W",Subject: "da sampling at most 1000ml, wcr at most 1000ml" @12:46:36.38
Loading sh1 puck from tube 3 into Collection Clamp @12:46:36.74 SC.reconfigure :tight @12:46:36.79 SC.seek 3 @12:46:40.36 SC.configure :limp @12:46:40.62 Elbow.reconfigure :tight @12:46:40.63 upsync @12:46:40.69
Elbow.seek 3 @12:46:42.64 Forearm.reconfigure :tight @12:46:42.69 Forearm.seek 3 + 450 counts @12:46:43.71 SE.reconfigure :pick @12:46:43.77 SE.seek :beamBreak @12:46:46.58 SE.configure :default @12:46:46.75 Elbow.seek 3 @12:46:46.94 Forearm.seek 3 + 300 counts @12:46:47.15 Forearm.reconfigure :loose @12:46:47.19 Hand.close @12:46:47.86 SE.seek :down @12:46:48.07
Forearm.seek :retract @12:46:49.45 Elbow.seek CC @12:46:50.30 SE.stop @12:46:51.08
Elbow.reconfigure :loose @12:46:51.18 Forearm.reconfigure :tight @12:46:51.23 Forearm.seek CC @12:46:52.56 CC.seek :closed @12:46:57.09 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.35 @12:46:57.10 Hand.open @12:46:57.78 Forearm.reconfigure :loose @12:46:57.83 Forearm.seek :retract @12:46:58.85 collectSample 1000ml w/bubble point of 24psi @12:46:58.86 Priming 7ml @12:46:58.87 Intake.select :close @12:46:58.88 Exhaust.select :close @12:46:58.90 SS.hold @12:46:59.11 Calibrating pressure and temperature sensors @12:46:59.14 CTV.dial :air @12:46:59.21 SSV.dial :spit,avoiding: :exhaust @12:46:59.29 SS.reconfigure :fast @12:46:59.34 SS.seek 5ml @12:47:02.17 SSV.dial CBV @12:47:02.19
CBV.dial SSV @12:47:03.55 delay 3.8 seconds @12:47:07.47 Sensor limits: Intake max 106.1psia; Outlet max 98.4psia @12:47:07.48 SSV.dial :spit @12:47:08.82 SS.seek 0ml @12:47:11.61 SSV.dial CBV @12:47:12.92 CTV.dial :intake @12:47:13.72 SSV.dial CBV @12:47:13.75
CBV.dial SSV @12:47:13.84 Allowing 40s for 7.0ml of remaining 7.0ml @12:47:13.85 Intake.select :open @12:47:16.01 Intake @14.7psia, vacuum from 9.2 to 12.2psi @12:47:16.02 SS.reconfigure :inhaling 2.20ml/s @12:47:16.07 SS.seek 7ml @12:47:22.82 Stroke completed -- waiting for pressure to equalize ... @12:47:22.83 SS.reconfigure :exhale @12:47:22.88 SS.hold @12:47:28.68 Intake.select :close @12:47:28.84 SSV.dial :exhaust @12:47:29.61 Exhaust.select :open @12:47:29.72 SS.seek 0ml @12:47:34.00 Exhaust.select :close @12:47:34.10 Sampling 1000.0ml @12:47:34.11 Intake.select :close @12:47:34.12 Exhaust.select :close @12:47:34.13 SS.reconfigure :default @12:47:34.18 SS.hold @12:47:34.38 CTV.dial :intake @12:47:34.61 SSV.dial CBV @12:47:34.65
CBV.dial SSV @12:47:35.40 Allowing 130s for 25.0ml of remaining 1000.0ml @12:47:35.41 Intake.select :open @12:47:37.58 Intake @15.2psia, vacuum from 9.7 to 12.7psi @12:47:37.59 SS.reconfigure :inhaling 2.20ml/s @12:47:37.64 SS.seek 25ml @12:47:46.24 Vacuum too high -- waiting up to 40 seconds @12:47:46.25 SS.reconfigure :exhale @12:47:46.30 SS.hold @12:47:51.51 Intake @15.6psia, vacuum from 10.0 to 13.0psi @12:47:51.53 SS.reconfigure :inhaling 1.76ml/s @12:47:51.57 SS.seek 25ml @12:47:59.37 Stroke completed -- waiting for pressure to equalize ... @12:47:59.38 SS.reconfigure :exhale @12:47:59.43 SS.hold @12:48:19.99 Intake.select :close @12:48:20.14 SSV.dial :exhaust @12:48:20.92 Exhaust.select :open @12:48:21.03 SS.seek 0ml @12:48:30.03 Exhaust.select :close @12:48:30.13 SSV.dial CBV @12:48:30.15
CBV.dial SSV @12:48:30.92 Allowing 130s for 25.0ml of remaining 975.0ml @12:48:30.93 Intake.select :open @12:48:33.10 Intake @15.8psia, vacuum from 10.0 to 13.0psi @12:48:33.11 SS.reconfigure :inhaling 1.76ml/s @12:48:33.16 SS.seek 25ml @12:48:42.96 Vacuum too high -- waiting up to 40 seconds @12:48:42.97 SS.reconfigure :exhale @12:48:43.02 SS.hold @12:48:49.08 Intake @17.3psia, vacuum from 10.0 to 13.0psi @12:48:49.10 SS.reconfigure :inhaling 1.41ml/s @12:48:49.15 SS.seek 25ml @12:48:53.68 Vacuum too high -- waiting up to 40 seconds @12:48:53.70 SS.reconfigure :exhale @12:48:53.75 SS.hold @12:48:56.36 Intake @17.1psia, vacuum from 10.0 to 13.0psi @12:48:56.38 SS.reconfigure :inhaling 1.13ml/s @12:48:56.43 SS.seek 25ml @12:49:02.66 Stroke completed -- waiting for pressure to equalize ... @12:49:02.67 SS.reconfigure :exhale @12:49:02.72 SS.hold @12:49:19.12 Intake.select :close @12:49:19.27 SSV.dial :exhaust @12:49:20.04 Exhaust.select :open @12:49:20.16 SS.seek 0ml @12:49:29.13 Exhaust.select :close @12:49:29.24 SSV.dial CBV @12:49:29.28
CBV.dial SSV @12:49:30.04 Allowing 130s for 25.0ml of remaining 950.0ml @12:49:30.05 Intake.select :open @12:49:32.22 Intake @16.1psia, vacuum from 10.0 to 13.0psi @12:49:32.23 SS.reconfigure :inhaling 1.13ml/s @12:49:32.28 SS.seek 25ml @12:49:56.74 Stroke completed -- waiting for pressure to equalize ... @12:49:56.75 SS.reconfigure :exhale @12:49:56.80 SS.hold @12:50:17.25 Intake.select :close @12:50:17.41 SSV.dial :exhaust @12:50:18.19 Exhaust.select :open @12:50:18.30 SS.seek 0ml @12:50:27.10 Exhaust.select :close @12:50:27.21 SSV.dial CBV @12:50:27.23
CBV.dial SSV @12:50:28.01 Allowing 130s for 25.0ml of remaining 925.0ml @12:50:28.02 Intake.select :open @12:50:30.19 Intake @16.0psia, vacuum from 10.0 to 13.0psi @12:50:30.20 SS.reconfigure :inhaling 1.13ml/s @12:50:30.25 SS.seek 25ml @12:50:55.07 Stroke completed -- waiting for pressure to equalize ... @12:50:55.08 SS.reconfigure :exhale @12:50:55.13 SS.hold @12:51:13.41 Intake.select :close @12:51:13.56 SSV.dial :exhaust @12:51:14.32 Exhaust.select :open @12:51:14.44 SS.seek 0ml @12:51:23.44 Exhaust.select :close @12:51:23.98 SSV.dial CBV @12:51:24.00
CBV.dial SSV @12:51:24.79 Allowing 130s for 25.0ml of remaining 900.0ml @12:51:24.80 Intake.select :open @12:51:26.97 Intake @16.1psia, vacuum from 10.0 to 13.0psi @12:51:26.98 SS.reconfigure :inhaling 1.13ml/s @12:51:27.03 SS.seek 25ml @12:51:51.40 Stroke completed -- waiting for pressure to equalize ... @12:51:51.41 SS.reconfigure :exhale @12:51:51.45 SS.hold @12:52:09.83 Intake.select :close @12:52:09.98 SSV.dial :exhaust @12:52:10.74 Exhaust.select :open @12:52:10.86 SS.seek 0ml @12:52:19.58 Exhaust.select :close @12:52:19.69 SSV.dial CBV @12:52:19.72
CBV.dial SSV @12:52:20.51 Allowing 130s for 25.0ml of remaining 875.0ml @12:52:20.52 Intake.select :open @12:52:22.70 Intake @16.0psia, vacuum from 10.0 to 13.0psi @12:52:22.71 SS.reconfigure :inhaling 1.13ml/s @12:52:22.76 SS.seek 25ml @12:52:47.40 Stroke completed -- waiting for pressure to equalize ... @12:52:47.41 SS.reconfigure :exhale @12:52:47.45 SS.hold @12:53:04.01 Intake.select :close @12:53:04.16 SSV.dial :exhaust @12:53:04.91 Exhaust.select :open @12:53:05.03 SS.seek 0ml @12:53:13.72 Exhaust.select :close @12:53:13.83 SSV.dial CBV @12:53:13.85
CBV.dial SSV @12:53:14.65 Allowing 130s for 25.0ml of remaining 850.0ml @12:53:14.66 Intake.select :open @12:53:16.83 Intake @16.0psia, vacuum from 10.0 to 13.0psi @12:53:16.84 SS.reconfigure :inhaling 1.13ml/s @12:53:16.89 SS.seek 25ml @12:53:41.41 Stroke completed -- waiting for pressure to equalize ... @12:53:41.42 SS.reconfigure :exhale @12:53:41.47 SS.hold @12:53:57.71 Intake.select :close @12:53:57.87 SSV.dial :exhaust @12:53:58.63 Exhaust.select :open @12:53:58.74 SS.seek 0ml @12:54:07.75 Exhaust.select :close @12:54:07.86 SSV.dial CBV @12:54:07.89
CBV.dial SSV @12:54:08.68 Allowing 130s for 25.0ml of remaining 825.0ml @12:54:08.69 Intake.select :open @12:54:10.86 Intake @15.9psia, vacuum from 10.0 to 13.0psi @12:54:10.87 SS.reconfigure :inhaling 1.13ml/s @12:54:10.92 SS.seek 25ml @12:54:35.08 Stroke completed -- waiting for pressure to equalize ... @12:54:35.09 SS.reconfigure :exhale @12:54:35.14 SS.hold @12:54:51.33 Intake.select :close @12:54:51.49 SSV.dial :exhaust @12:54:52.27 Exhaust.select :open @12:54:52.38 SS.seek 0ml @12:55:01.36 Exhaust.select :close @12:55:01.47 SSV.dial CBV @12:55:01.49
CBV.dial SSV @12:55:02.27 Allowing 130s for 25.0ml of remaining 800.0ml @12:55:02.28 Intake.select :open @12:55:04.45 Intake @15.9psia, vacuum from 10.0 to 13.0psi @12:55:04.46 SS.reconfigure :inhaling 1.13ml/s @12:55:04.51 SS.seek 25ml @12:55:28.66 Stroke completed -- waiting for pressure to equalize ... @12:55:28.67 SS.reconfigure :exhale @12:55:28.71 SS.hold @12:55:44.99 Intake.select :close @12:55:45.15 SSV.dial :exhaust @12:55:45.91 Exhaust.select :open @12:55:46.02 SS.seek 0ml @12:55:54.75 Exhaust.select :close @12:55:54.86 SSV.dial CBV @12:55:54.88
CBV.dial SSV @12:55:55.66 Allowing 130s for 25.0ml of remaining 775.0ml @12:55:55.67 Intake.select :open @12:55:57.84 Intake @16.0psia, vacuum from 10.0 to 13.0psi @12:55:57.86 SS.reconfigure :inhaling 1.13ml/s @12:55:57.90 SS.seek 25ml @12:56:19.38 Vacuum too high -- waiting up to 40 seconds @12:56:19.40 SS.reconfigure :exhale @12:56:19.45 SS.hold @12:56:27.71 Intake @16.6psia, vacuum from 10.0 to 13.0psi @12:56:27.72 SS.reconfigure :inhaling 0.91ml/s @12:56:27.77 SS.seek 25ml @12:56:32.02 Stroke completed -- waiting for pressure to equalize ... @12:56:32.03 SS.reconfigure :exhale @12:56:32.07 SS.hold @12:56:40.69 Intake.select :close @12:56:40.84 SSV.dial :exhaust @12:56:41.63 Exhaust.select :open @12:56:41.74 SS.seek 0ml @12:56:50.61 Exhaust.select :close @12:56:50.72 SSV.dial CBV @12:56:50.74
CBV.dial SSV @12:56:51.52 Allowing 130s for 25.0ml of remaining 750.0ml @12:56:51.53 Intake.select :open @12:56:53.69 Intake @15.9psia, vacuum from 10.0 to 13.0psi @12:56:53.71 SS.reconfigure :inhaling 0.91ml/s @12:56:53.76 SS.seek 25ml @12:57:23.00 Stroke completed -- waiting for pressure to equalize ... @12:57:23.01 SS.reconfigure :exhale @12:57:23.06 SS.hold @12:57:37.33 Intake.select :close @12:57:37.92 SSV.dial :exhaust @12:57:38.69 Exhaust.select :open @12:57:38.80 SS.seek 0ml @12:57:47.77 Exhaust.select :close @12:57:47.88 SSV.dial CBV @12:57:47.92
CBV.dial SSV @12:57:48.67 Allowing 130s for 25.0ml of remaining 725.0ml @12:57:48.68 Intake.select :open @12:57:50.85 Intake @15.9psia, vacuum from 10.0 to 13.0psi @12:57:50.87 SS.reconfigure :inhaling 0.91ml/s @12:57:50.92 SS.seek 25ml @12:58:20.51 Stroke completed -- waiting for pressure to equalize ... @12:58:20.52 SS.reconfigure :exhale @12:58:20.57 SS.hold @12:58:34.96 Intake.select :close @12:58:35.12 SSV.dial :exhaust @12:58:35.87 Exhaust.select :open @12:58:35.99 SS.seek 0ml @12:58:44.72 Exhaust.select :close @12:58:44.82 SSV.dial CBV @12:58:44.84
CBV.dial SSV @12:58:45.63 Allowing 130s for 25.0ml of remaining 700.0ml @12:58:45.64 Intake.select :open @12:58:47.80 Intake @15.9psia, vacuum from 10.0 to 13.0psi @12:58:47.82 SS.reconfigure :inhaling 0.91ml/s @12:58:47.87 SS.seek 25ml @12:59:17.75 Stroke completed -- waiting for pressure to equalize ... @12:59:17.76 SS.reconfigure :exhale @12:59:17.85 SS.hold @12:59:32.52 Intake.select :close @12:59:32.68 SSV.dial :exhaust @12:59:33.46 Exhaust.select :open @12:59:33.57 SS.seek 0ml @12:59:42.51 Exhaust.select :close @12:59:42.62 SSV.dial CBV @12:59:42.65
CBV.dial SSV @12:59:43.42 Allowing 130s for 25.0ml of remaining 675.0ml @12:59:43.43 Intake.select :open @12:59:45.60 Intake @15.9psia, vacuum from 10.0 to 13.0psi @12:59:45.61 SS.reconfigure :inhaling 0.91ml/s @12:59:45.66 SS.seek 25ml @13:00:15.07 Stroke completed -- waiting for pressure to equalize ... @13:00:15.08 SS.reconfigure :exhale @13:00:15.13 SS.hold @13:00:31.49 Intake.select :close @13:00:31.64 SSV.dial :exhaust @13:00:32.40 Exhaust.select :open @13:00:32.52 SS.seek 0ml @13:00:41.23 Exhaust.select :close @13:00:41.34 SSV.dial CBV @13:00:41.36
CBV.dial SSV @13:00:42.15 Allowing 130s for 25.0ml of remaining 650.0ml @13:00:42.17 Intake.select :open @13:00:44.33 Intake @15.8psia, vacuum from 10.0 to 13.0psi @13:00:44.35 SS.reconfigure :inhaling 0.91ml/s @13:00:44.40 SS.seek 25ml @13:01:14.18 Stroke completed -- waiting for pressure to equalize ... @13:01:14.19 SS.reconfigure :exhale @13:01:14.24 SS.hold @13:01:32.69 Intake.select :close @13:01:32.84 SSV.dial :exhaust @13:01:33.62 Exhaust.select :open @13:01:33.74 SS.seek 0ml @13:01:42.49 Exhaust.select :close @13:01:42.60 SSV.dial CBV @13:01:42.64
CBV.dial SSV @13:01:43.39 Allowing 130s for 25.0ml of remaining 625.0ml @13:01:43.40 Intake.select :open @13:01:45.56 Intake @15.8psia, vacuum from 10.0 to 13.0psi @13:01:45.57 SS.reconfigure :inhaling 0.91ml/s @13:01:45.62 SS.seek 25ml @13:02:15.49 Stroke completed -- waiting for pressure to equalize ... @13:02:15.50 SS.reconfigure :exhale @13:02:15.55 SS.hold @13:02:36.02 Intake.select :close @13:02:36.17 SSV.dial :exhaust @13:02:36.94 Exhaust.select :open @13:02:37.05 SS.seek 0ml @13:02:46.01 Exhaust.select :close @13:02:46.12 SSV.dial CBV @13:02:46.14
CBV.dial SSV @13:02:46.92 Allowing 130s for 25.0ml of remaining 600.0ml @13:02:46.93 Intake.select :open @13:02:49.10 Intake @15.8psia, vacuum from 10.0 to 13.0psi @13:02:49.11 SS.reconfigure :inhaling 0.91ml/s @13:02:49.16 SS.seek 25ml @13:03:11.34 Vacuum too high -- waiting up to 40 seconds @13:03:11.36 SS.reconfigure :exhale @13:03:11.41 SS.hold @13:03:21.28 Intake @16.0psia, vacuum from 10.0 to 13.0psi @13:03:21.30 SS.reconfigure :inhaling 0.73ml/s @13:03:21.34 SS.seek 25ml @13:03:30.65 Stroke completed -- waiting for pressure to equalize ... @13:03:30.66 SS.reconfigure :exhale @13:03:30.70 SS.hold @13:03:43.79 Intake.select :close @13:03:43.94 SSV.dial :exhaust @13:03:44.73 Exhaust.select :open @13:03:44.84 SS.seek 0ml @13:03:53.85 Exhaust.select :close @13:03:53.97 SSV.dial CBV @13:03:53.99
CBV.dial SSV @13:03:54.76 Allowing 130s for 25.0ml of remaining 575.0ml @13:03:54.77 Intake.select :open @13:03:56.94 Intake @15.8psia, vacuum from 10.0 to 13.0psi @13:03:56.96 SS.reconfigure :inhaling 0.73ml/s @13:03:57.01 SS.seek 25ml @13:04:32.94 Stroke completed -- waiting for pressure to equalize ... @13:04:32.95 SS.reconfigure :exhale @13:04:33.00 SS.hold @13:04:53.38 Intake.select :close @13:04:53.54 SSV.dial :exhaust @13:04:54.31 Exhaust.select :open @13:04:54.43 SS.seek 0ml @13:05:03.23 Exhaust.select :close @13:05:03.34 SSV.dial CBV @13:05:03.38
CBV.dial SSV @13:05:04.12 Allowing 130s for 25.0ml of remaining 550.0ml @13:05:04.13 Intake.select :open @13:05:06.29 Intake @15.8psia, vacuum from 10.0 to 13.0psi @13:05:06.31 SS.reconfigure :inhaling 0.73ml/s @13:05:06.36 SS.seek 25ml @13:05:42.82 Stroke completed -- waiting for pressure to equalize ... @13:05:42.83 SS.reconfigure :exhale @13:05:42.87 SS.hold @13:06:03.67 Intake.select :close @13:06:03.82 SSV.dial :exhaust @13:06:04.61 Exhaust.select :open @13:06:04.72 SS.seek 0ml @13:06:13.71 Exhaust.select :close @13:06:13.81 SSV.dial CBV @13:06:13.84
CBV.dial SSV @13:06:14.60 Allowing 130s for 25.0ml of remaining 525.0ml @13:06:14.61 Intake.select :open @13:06:16.78 Intake @15.8psia, vacuum from 10.0 to 13.0psi @13:06:16.79 SS.reconfigure :inhaling 0.73ml/s @13:06:16.84 SS.seek 25ml @13:06:52.18 Vacuum too high -- waiting up to 40 seconds @13:06:52.20 SS.reconfigure :exhale @13:06:52.25 SS.hold @13:07:03.94 Intake @15.9psia, vacuum from 10.0 to 13.0psi @13:07:03.96 SS.reconfigure :inhaling 0.59ml/s @13:07:04.01 SS.seek 25ml @13:07:04.88 Stroke completed -- waiting for pressure to equalize ... @13:07:04.89 SS.reconfigure :exhale @13:07:04.94 SS.hold @13:07:16.06 Intake.select :close @13:07:16.22 SSV.dial :exhaust @13:07:16.97 Exhaust.select :open @13:07:17.09 SS.seek 0ml @13:07:26.16 Exhaust.select :close @13:07:26.27 SSV.dial CBV @13:07:26.29
CBV.dial SSV @13:07:27.09 Allowing 130s for 25.0ml of remaining 500.0ml @13:07:27.10 Intake.select :open @13:07:29.26 Intake @15.7psia, vacuum from 10.0 to 13.0psi @13:07:29.27 SS.reconfigure :inhaling 0.59ml/s @13:07:29.32 SS.seek 25ml @13:08:13.91 Stroke completed -- waiting for pressure to equalize ... @13:08:13.92 SS.reconfigure :exhale @13:08:13.96 SS.hold @13:08:34.56 Intake.select :close @13:08:34.71 SSV.dial :exhaust @13:08:35.49 Exhaust.select :open @13:08:35.61 SS.seek 0ml @13:08:44.44 Exhaust.select :close @13:08:44.55 SSV.dial CBV @13:08:44.57
CBV.dial SSV @13:08:45.35 Allowing 130s for 25.0ml of remaining 475.0ml @13:08:45.36 Intake.select :open @13:08:47.52 Intake @15.7psia, vacuum from 10.0 to 13.0psi @13:08:47.53 SS.reconfigure :inhaling 0.59ml/s @13:08:47.58 SS.seek 25ml @13:09:31.97 Stroke completed -- waiting for pressure to equalize ... @13:09:31.98 SS.reconfigure :exhale @13:09:32.03 SS.hold @13:09:54.58 Intake.select :close @13:09:54.74 SSV.dial :exhaust @13:09:55.50 Exhaust.select :open @13:09:55.62 SS.seek 0ml @13:10:04.42 Exhaust.select :close @13:10:04.53 SSV.dial CBV @13:10:04.55
CBV.dial SSV @13:10:05.38 Allowing 130s for 25.0ml of remaining 450.0ml @13:10:05.39 Intake.select :open @13:10:07.55 Intake @15.8psia, vacuum from 10.0 to 13.0psi @13:10:07.57 SS.reconfigure :inhaling 0.59ml/s @13:10:07.62 SS.seek 25ml @13:10:52.08 Vacuum too high -- waiting up to 40 seconds @13:10:52.10 SS.reconfigure :exhale @13:10:52.15 SS.hold @13:11:05.22 Intake @16.2psia, vacuum from 10.0 to 13.0psi @13:11:05.24 SS.reconfigure :inhaling 0.47ml/s @13:11:05.28 SS.seek 25ml @13:11:05.95 Stroke completed -- waiting for pressure to equalize ... @13:11:05.96 SS.reconfigure :exhale @13:11:06.01 SS.hold @13:11:18.66 Intake.select :close @13:11:18.81 SSV.dial :exhaust @13:11:19.58 Exhaust.select :open @13:11:20.12 SS.seek 0ml @13:11:28.90 Exhaust.select :close @13:11:29.01 SSV.dial CBV @13:11:29.03
CBV.dial SSV @13:11:29.81 Allowing 130s for 25.0ml of remaining 425.0ml @13:11:29.83 Intake.select :open @13:11:32.00 Intake @15.8psia, vacuum from 10.0 to 13.0psi @13:11:32.01 SS.reconfigure :inhaling 0.47ml/s @13:11:32.06 SS.seek 25ml @13:12:27.20 Stroke completed -- waiting for pressure to equalize ... @13:12:27.21 SS.reconfigure :exhale @13:12:27.26 SS.hold @13:12:47.69 Intake.select :close @13:12:47.84 SSV.dial :exhaust @13:12:48.60 Exhaust.select :open @13:12:48.71 SS.seek 0ml @13:12:57.45 Exhaust.select :close @13:12:57.56 SSV.dial CBV @13:12:57.58
CBV.dial SSV @13:12:58.37 Allowing 130s for 25.0ml of remaining 400.0ml @13:12:58.39 Intake.select :open @13:13:00.97 Intake @15.7psia, vacuum from 10.0 to 13.0psi @13:13:00.98 SS.reconfigure :inhaling 0.47ml/s @13:13:01.03 SS.seek 25ml @13:13:57.03 Stroke completed -- waiting for pressure to equalize ... @13:13:57.04 SS.reconfigure :exhale @13:13:57.08 SS.hold @13:14:21.48 Intake.select :close @13:14:21.63 SSV.dial :exhaust @13:14:22.40 Exhaust.select :open @13:14:22.52 SS.seek 0ml @13:14:31.31 Exhaust.select :close @13:14:31.42 SSV.dial CBV @13:14:31.46
CBV.dial SSV @13:14:32.23 Allowing 130s for 25.0ml of remaining 375.0ml @13:14:32.24 Intake.select :open @13:14:34.41 Intake @15.9psia, vacuum from 10.0 to 13.0psi @13:14:34.78 SS.reconfigure :inhaling 0.47ml/s @13:14:34.90 SS.seek 25ml @13:15:30.59 Stroke completed -- waiting for pressure to equalize ... @13:15:30.60 SS.reconfigure :exhale @13:15:30.65 SS.hold @13:15:57.07 Intake.select :close @13:15:57.23 SSV.dial :exhaust @13:15:58.02 Exhaust.select :open @13:15:58.13 SS.seek 0ml @13:16:07.17 Exhaust.select :close @13:16:07.28 SSV.dial CBV @13:16:07.30
CBV.dial SSV @13:16:08.07 Allowing 130s for 25.0ml of remaining 350.0ml @13:16:08.08 Intake.select :open @13:16:10.60 Intake @15.8psia, vacuum from 10.0 to 13.0psi @13:16:10.69 SS.reconfigure :inhaling 0.47ml/s @13:16:10.74 SS.seek 25ml @13:17:06.07 Stroke completed -- waiting for pressure to equalize ... @13:17:06.08 SS.reconfigure :exhale @13:17:06.13 SS.hold @13:17:36.78 Intake.select :close @13:17:36.93 SSV.dial :exhaust @13:17:37.70 Exhaust.select :open @13:17:37.81 SS.seek 0ml @13:17:46.85 Exhaust.select :close @13:17:47.32 SSV.dial CBV @13:17:47.36
CBV.dial SSV @13:17:48.13 Allowing 130s for 25.0ml of remaining 325.0ml @13:17:48.15 Intake.select :open @13:17:50.31 Intake @15.9psia, vacuum from 10.0 to 13.0psi @13:17:50.33 SS.reconfigure :inhaling 0.47ml/s @13:17:50.37 SS.seek 25ml @13:18:40.39 Vacuum too high -- waiting up to 40 seconds @13:18:40.40 SS.reconfigure :exhale @13:18:40.45 SS.hold @13:18:56.91 Intake @16.5psia, vacuum from 10.0 to 13.0psi @13:18:56.93 SS.reconfigure :inhaling 0.38ml/s @13:18:56.97 SS.seek 25ml @13:19:03.61 Stroke completed -- waiting for pressure to equalize ... @13:19:03.62 SS.reconfigure :exhale @13:19:03.67 SS.hold @13:19:20.09 Intake.select :close @13:19:20.24 SSV.dial :exhaust @13:19:21.02 Exhaust.select :open @13:19:21.14 SS.seek 0ml @13:19:29.93 Exhaust.select :close @13:19:30.03 SSV.dial CBV @13:19:30.05
CBV.dial SSV @13:19:30.82 Allowing 130s for 25.0ml of remaining 300.0ml @13:19:30.83 Intake.select :open @13:19:33.00 Intake @16.0psia, vacuum from 10.0 to 13.0psi @13:19:33.01 SS.reconfigure :inhaling 0.38ml/s @13:19:33.06 SS.seek 25ml @13:20:42.67 Stroke completed -- waiting for pressure to equalize ... @13:20:42.68 SS.reconfigure :exhale @13:20:42.73 SS.hold @13:21:11.34 Intake.select :close @13:21:11.49 SSV.dial :exhaust @13:21:12.25 Exhaust.select :open @13:21:12.37 SS.seek 0ml @13:21:21.19 Exhaust.select :close @13:21:21.30 SSV.dial CBV @13:21:21.32
CBV.dial SSV @13:21:22.11 Allowing 130s for 25.0ml of remaining 275.0ml @13:21:22.13 Intake.select :open @13:21:24.29 Intake @15.9psia, vacuum from 10.0 to 13.0psi @13:21:24.30 SS.reconfigure :inhaling 0.38ml/s @13:21:24.35 SS.seek 25ml @13:22:33.73 Stroke completed -- waiting for pressure to equalize ... @13:22:33.74 SS.reconfigure :exhale @13:22:33.79 SS.hold @13:23:04.89 Intake.select :close @13:23:05.04 SSV.dial :exhaust @13:23:05.82 Exhaust.select :open @13:23:05.94 SS.seek 0ml @13:23:14.70 Exhaust.select :close @13:23:14.81 SSV.dial CBV @13:23:14.84
CBV.dial SSV @13:23:15.63 Allowing 130s for 25.0ml of remaining 250.0ml @13:23:15.64 Intake.select :open @13:23:17.81 Intake @15.8psia, vacuum from 10.0 to 13.0psi @13:23:17.82 SS.reconfigure :inhaling 0.38ml/s @13:23:17.87 SS.seek 25ml @13:24:27.08 Stroke completed -- waiting for pressure to equalize ... @13:24:27.09 SS.reconfigure :exhale @13:24:27.14 SS.hold @13:25:01.83 Intake.select :close @13:25:01.99 SSV.dial :exhaust @13:25:02.75 Exhaust.select :open @13:25:02.86 SS.seek 0ml @13:25:11.87 Exhaust.select :close @13:25:11.98 SSV.dial CBV @13:25:12.00
CBV.dial SSV @13:25:12.80 Allowing 130s for 25.0ml of remaining 225.0ml @13:25:12.81 Intake.select :open @13:25:15.02 Intake @15.9psia, vacuum from 10.0 to 13.0psi @13:25:15.04 SS.reconfigure :inhaling 0.38ml/s @13:25:15.09 SS.seek 25ml @13:26:23.68 Stroke completed -- waiting for pressure to equalize ... @13:26:23.69 SS.reconfigure :exhale @13:26:23.74 SS.hold @13:27:00.92 Intake.select :close @13:27:01.08 SSV.dial :exhaust @13:27:01.84 Exhaust.select :open @13:27:01.96 SS.seek 0ml @13:27:11.18 Exhaust.select :close @13:27:11.29 SSV.dial CBV @13:27:11.33
CBV.dial SSV @13:27:12.09 Allowing 130s for 25.0ml of remaining 200.0ml @13:27:12.10 Intake.select :open @13:27:14.69 Intake @15.9psia, vacuum from 10.0 to 13.0psi @13:27:14.71 SS.reconfigure :inhaling 0.38ml/s @13:27:14.76 SS.seek 25ml @13:28:15.97 Vacuum too high -- waiting up to 40 seconds @13:28:15.99 SS.reconfigure :exhale @13:28:16.04 SS.hold @13:28:36.43 Intake @16.5psia, vacuum from 10.0 to 13.0psi @13:28:36.44 SS.reconfigure :inhaling 0.31ml/s @13:28:36.49 SS.seek 25ml @13:28:45.27 Stroke completed -- waiting for pressure to equalize ... @13:28:45.28 SS.reconfigure :exhale @13:28:45.33 SS.hold @13:29:10.23 Intake.select :close @13:29:10.38 SSV.dial :exhaust @13:29:11.15 Exhaust.select :open @13:29:11.27 SS.seek 0ml @13:29:20.28 Exhaust.select :close @13:29:20.38 SSV.dial CBV @13:29:20.41
CBV.dial SSV @13:29:21.18 Allowing 130s for 25.0ml of remaining 175.0ml @13:29:21.20 Intake.select :open @13:29:23.36 Intake @15.9psia, vacuum from 10.0 to 13.0psi @13:29:23.38 SS.reconfigure :inhaling 0.31ml/s @13:29:23.43 SS.seek 25ml @13:30:52.44 Stroke completed -- waiting for pressure to equalize ... @13:30:52.45 SS.reconfigure :exhale @13:30:52.50 SS.hold @13:31:25.25 Intake.select :close @13:31:25.41 SSV.dial :exhaust @13:31:26.17 Exhaust.select :open @13:31:26.29 SS.seek 0ml @13:31:35.38 Exhaust.select :close @13:31:35.49 SSV.dial CBV @13:31:35.51
CBV.dial SSV @13:31:36.31 Allowing 130s for 25.0ml of remaining 150.0ml @13:31:36.32 Intake.select :open @13:31:38.48 Intake @16.0psia, vacuum from 10.0 to 13.0psi @13:31:38.49 SS.reconfigure :inhaling 0.31ml/s @13:31:38.54 SS.seek 25ml @13:33:07.89 Stroke completed -- waiting for pressure to equalize ... @13:33:07.90 SS.reconfigure :exhale @13:33:07.95 SS.hold @13:33:46.33 SS.reconfigure :default @13:33:46.37 SS.hold @13:33:46.43 Intake.select :close @13:33:46.59 ShallowSampler::Clogged in MAIN -- Filtering too Slowly after 875.0ml @13:33:46.60 SSV.dial :exhaust @13:33:47.37 Exhaust.select :open @13:33:47.48 SS.reconfigure :exhale @13:33:47.53 SS.seek 0ml @13:33:56.55 Exhaust.select :close @13:33:56.66 Sampled 875.0ml @13:33:56.67 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @13:33:56.68 Intake.select :close @13:33:57.18 CSV.dial :waste @13:33:57.34 SSV.dial CBV,avoiding: :exhaust @13:33:57.37 CS.seek 0ml @13:33:57.44
CBV.dial SSV @13:33:57.51 CSV.dial :air @13:33:58.25 CS.seek 10ml @13:33:59.31
CTV.dial :air @13:34:00.07 delay 15 seconds @13:34:15.09 SSV.dial :spit,avoiding: :exhaust @13:34:16.44 CTV.dial CSV,avoiding: :intake @13:34:16.49
SS.seek 0ml @13:34:16.59 SSV.dial CBV,avoiding: :exhaust @13:34:22.98 SSV.dial between CBV and plug @13:34:23.46 CBV.dial between SSV and waste2 @13:34:23.85 CSV.dial between air and CTV @13:34:24.17 CS.seek 8.395ml @13:34:28.64 CTV.dial CSV,avoiding: :air @13:34:28.66
CSV.dial CTV @13:34:29.01 CS.reconfigure :pressurize @13:34:29.06 CS.seek 0ml @13:34:42.29 BubblePoint: Intake @24.2psia, Outlet @1.1psia @13:34:42.30 CBV.dial SSV @13:34:42.65 SS.reconfigure :default @13:34:42.70 SS.seek 10ml @13:34:48.22 delay 15 seconds @13:35:03.35 Evacuated: Intake @23.7psia, Outlet @23.6psia @13:35:03.36 SSV.dial :spit,avoiding: :exhaust @13:35:04.40 SS.reconfigure :exhale @13:35:04.45 SS.seek 0ml @13:35:09.40 SS.reconfigure :fast @13:35:09.44 SS.seek 5ml @13:35:12.22 SSV.dial CBV,avoiding: :exhaust @13:35:12.23
CBV.dial SSV @13:35:13.62 CTV.dial :air,avoiding: :intake @13:35:14.98 CSV.dial CTV @13:35:15.02
SS.seek 10ml @13:35:16.14 CSV.dial :air @13:35:16.59 CS.reconfigure :fast @13:35:16.63 CS.seek 0ml @13:35:17.52
SSV.dial :spit,avoiding: :exhaust @13:35:18.87 SS.reconfigure :exhale @13:35:18.92 SS.seek 0ml @13:35:23.97 SS.reconfigure :fast @13:35:24.01 SS.seek 5ml @13:35:25.53 CS.stop @13:35:27.18 SSV.dial CBV,avoiding: :exhaust @13:35:27.20
CBV.dial SSV @13:35:29.61 SSV.dial :spit,avoiding: :exhaust @13:35:30.96 SS.reconfigure :default @13:35:31.01 SS.seek 0ml @13:35:34.22 SS.stop @13:35:34.26 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @13:35:34.27 Intake.select :close @13:35:34.34 CSV.dial :waste @13:35:34.47 SSV.dial CBV,avoiding: :exhaust @13:35:34.49
CBV.dial SSV @13:35:35.12 CS.seek 0ml @13:35:35.19 CSV.dial :air @13:35:35.88
CTV.dial :air @13:35:35.95 CS.seek 10ml @13:35:36.11
delay 15 seconds @13:35:51.13 SSV.dial :spit,avoiding: :exhaust @13:35:52.46 CTV.dial CSV,avoiding: :intake @13:35:52.49
SS.reconfigure :exhale @13:35:52.60 SS.seek 0ml @13:35:53.00 SSV.dial CBV,avoiding: :exhaust @13:36:00.50 SSV.dial between CBV and plug @13:36:01.01 CBV.dial between SSV and waste2 @13:36:01.41 CSV.dial between air and CTV @13:36:01.73 CS.seek 7.365ml @13:36:08.57 CTV.dial CSV,avoiding: :air @13:36:08.59
CSV.dial CTV @13:36:08.93 CS.reconfigure :pressurize @13:36:08.98 CS.seek 0ml @13:36:20.98 BubblePoint: Intake @24.2psia, Outlet @4.1psia @13:36:20.99 CBV.dial SSV @13:36:21.34 SS.reconfigure :default @13:36:21.38 SS.seek 10ml @13:36:26.91 delay 15 seconds @13:36:42.04 Evacuated: Intake @23.8psia, Outlet @23.9psia @13:36:42.05 SSV.dial :spit,avoiding: :exhaust @13:36:43.09 SS.reconfigure :exhale @13:36:43.13 SS.seek 0ml @13:36:48.29 SS.reconfigure :fast @13:36:48.33 SS.seek 5ml @13:36:51.24 SSV.dial CBV,avoiding: :exhaust @13:36:51.26
CBV.dial SSV @13:36:52.63 CTV.dial :air,avoiding: :intake @13:36:54.41 CSV.dial CTV @13:36:54.45
SS.seek 10ml @13:36:55.65 CSV.dial :air @13:36:56.21 CS.reconfigure :fast @13:36:56.26 CS.seek 0ml @13:36:57.08
SSV.dial :spit,avoiding: :exhaust @13:36:58.44 SS.reconfigure :exhale @13:36:58.49 SS.seek 0ml @13:37:03.51 SS.reconfigure :fast @13:37:03.57 SS.seek 5ml @13:37:03.75 CS.stop @13:37:06.53 SSV.dial CBV,avoiding: :exhaust @13:37:06.54
CBV.dial SSV @13:37:08.93 SSV.dial :spit,avoiding: :exhaust @13:37:10.26 SS.reconfigure :default @13:37:10.30 SS.seek 0ml @13:37:13.36 SS.stop @13:37:13.40 Lysing: Adding 2.0ml AqMeOH @13:37:13.41 CBV.dial between CSV and waste1,avoiding: :DBV @13:37:14.91 CTV.dial CSV @13:37:16.24 CSV.dial CSR @13:37:17.01 Pulling 1ml of CSR.AqMeOH @13:37:17.02 CSR.select :AqMeOH @13:37:17.13 CS.reconfigure :default @13:37:17.18 CS.seek 1ml @13:37:20.60 delay 5 seconds @13:37:25.62 CSR.select :air @13:37:25.79 CS.seek 6ml @13:37:39.38 delay 2 seconds @13:37:41.39 CSV.dial :waste2 @13:37:41.85 CS.reconfigure :fast @13:37:41.90 CS.seek 0ml @13:37:56.38 CSV.dial CSR @13:37:56.95 Pulling 2ml of CSR.AqMeOH @13:37:56.96 CSR.select :AqMeOH @13:37:57.07 CS.reconfigure :default @13:37:57.12 CS.seek 2ml @13:38:03.25 delay 5 seconds @13:38:08.27 CSR.select :air @13:38:08.44 CS.seek 5ml @13:38:16.94 delay 2 seconds @13:38:18.95 CSV.dial CTV @13:38:19.43 CS.seek 1ml @13:38:30.67 Before lysing Collection Puck Heater at 13.9C/31.2psia @13:38:30.69 Heating Puck.from 3, :sh1 in Collection Clamp to 70C for 10:00 @13:38:30.71 CH.seek 70C,8:00 @13:40:44.89 CH.hold 10:00,70C @13:40:45.08 Begun lysing Collection Puck Heater at 68C/38.8psia @13:50:45.09 Ended lysing Collection Puck Heater at 70C/37.5psia @13:50:45.10 CS.seek 0ml @13:50:48.49 CTV.dial between spare and air,avoiding: :intake @13:50:49.52 CSV.dial :air @13:50:49.96 CS.seek 5ml @13:51:03.58 CSV.dial CTV @13:51:04.04 delay 2 seconds @13:51:06.05 CSV.dial :air @13:51:06.51 delay 2 seconds @13:51:08.52 CSV.dial CTV @13:51:08.97 delay 2 seconds @13:51:10.99 CSV.dial :air @13:51:11.44 delay 2 seconds @13:51:13.47 CS.seek 0ml @13:51:27.07 Puck @85.9psia after final lysis pressurization @13:51:27.09 Recovering Lysate into Collection syringe @13:51:27.10 CSV.dial CBV @13:51:28.11 CBV.dial CSV @13:51:28.50 CS.seek 5ml @13:51:42.30 delay 5 seconds @13:51:47.32 CTV.dial :air @13:51:47.83 CS.seek 7ml @13:51:53.80 delay 5 seconds @13:51:58.82 CSV.dial :air2 @13:51:59.26 CS.seek 10ml @13:52:07.71 CSV.dial between air and CTV,avoiding: CBV @13:52:08.87 Lysing Complete @13:52:08.94 Storing sh1 puck in Collection Clamp to tube 2 @13:52:09.11 SC.reconfigure :tight @13:52:09.15 SC.seek 2 @13:52:12.68 SC.configure :limp @13:52:12.90 Elbow.reconfigure :tight @13:52:12.95 Elbow.seek 2 @13:52:14.73 Forearm.reconfigure :tight @13:52:14.77 Forearm.seek 2 + 450 counts @13:52:16.14 SE.reconfigure :pick @13:52:16.26 SE.seek :beamBreak @13:52:17.71 SE.configure :default @13:52:17.89 Elbow.reconfigure :limp @13:52:17.94 Forearm.reconfigure :loose @13:52:17.98 Forearm.seek 2 @13:52:18.25 Elbow.hold @13:52:18.32 Elbow.reconfigure :tight @13:52:18.42 SE.seek 26.09 Pucks below Top Plate @13:52:18.94 Forearm.force -20 @13:52:18.98 Forearm.seek 2 + 299 counts @13:52:19.26 Forearm.reconfigure :tight @13:52:19.31 Forearm.seek 2 + 450 counts @13:52:19.51 SE.seek 27.09 Pucks below Top Plate @13:52:20.16 Forearm.reconfigure :loose @13:52:20.21 Forearm.seek :retract @13:52:21.18 Elbow.seek CC @13:52:22.84 Elbow.reconfigure :loose @13:52:22.93 Forearm.seek CC @13:52:23.91 Hand.close @13:52:24.55 CC.seek :open @13:52:29.28 Forearm.seek :retract @13:52:30.25 Elbow.reconfigure :tight @13:52:30.30 Elbow.seek 2 @13:52:32.09 Elbow.reconfigure :limp @13:52:32.14 Forearm.seek 2 @13:52:33.20 Elbow.hold @13:52:33.27 Elbow.reconfigure :tight @13:52:33.32 Hand.open @13:52:34.41 Forearm.force -20 @13:52:34.53 Forearm.seek 2 + 316 counts @13:52:34.83 Forearm.reconfigure :tight @13:52:34.88 Forearm.seek 2 + 450 counts @13:52:35.15 SE.seek :down @13:52:36.01 SE.stop @13:52:36.28
Forearm.reconfigure :loose @13:52:36.33 Forearm.seek :retract @13:52:37.32 Tranferring DA @13:52:37.33 Beginning samplePassC @13:52:37.34 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @13:52:37.53 Elbow.seek FlushPuck::Garage @13:52:39.36 Elbow.reconfigure :loose @13:52:39.46 Forearm.seek :garage @13:52:40.45 Hand.close @13:52:41.17 Forearm.seek :retract @13:52:42.16 Elbow.reconfigure :tight @13:52:42.21 Elbow.seek CC @13:52:45.15 Elbow.reconfigure :loose @13:52:45.25 Forearm.reconfigure :tight @13:52:45.30 Forearm.seek CC @13:52:46.53 CC.seek :closed @13:52:51.05 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.44 @13:52:51.12 Hand.open @13:52:52.24 Forearm.reconfigure :loose @13:52:52.29 Forearm.seek :retract @13:52:53.25 PSV.dial PSR @13:52:54.64 Pulling 1.5ml of PSR.flush @13:52:54.65 PSR.select :flush @13:52:54.77 PS.seek 1.5ml @13:53:11.02 delay 5 seconds @13:53:16.04 PSV.dial :waste1 @13:53:16.64 PS.seek 0ml @13:53:32.77 PSV.dial PSR @13:53:33.22 PSR.select :air @13:53:33.34 PS.seek 2.5ml @13:53:59.65 PRV.dial CBV,avoiding: :ab2 @13:53:59.67
CBV.dial PRV @13:54:01.43 PSV.dial :mixloop @13:54:02.48 CTV.dial :air,avoiding: :intake @13:54:02.55 PTV.dial :PRVmixing @13:54:03.89 PS.seek 0ml @13:54:30.24 PSV.dial :air @13:54:30.70 PS.seek 2.5ml @13:54:57.11 PSV.dial :mixloop @13:54:57.59 PS.seek 0ml @13:55:24.11 PSV.dial :air @13:55:24.59 PS.seek 2.5ml @13:55:50.99 PSV.dial :mixloop @13:55:51.46 PS.seek 0ml @13:56:17.86 Garaging FlushPuck in Collection Clamp @13:56:17.93 Elbow.reconfigure :tight @13:56:17.98 Elbow.seek CC @13:56:18.31 Elbow.reconfigure :loose @13:56:18.41 Forearm.seek CC @13:56:19.47 Hand.close @13:56:20.05 CC.seek :open @13:56:24.66 Forearm.seek :retract @13:56:25.66 Elbow.reconfigure :tight @13:56:25.70 Elbow.seek :garage @13:56:29.05 Elbow.reconfigure :loose @13:56:29.16 Forearm.reconfigure :tight @13:56:29.21 Forearm.seek :garage @13:56:30.31 Hand.open @13:56:30.98 Forearm.reconfigure :loose @13:56:31.03 Forearm.seek :retract @13:56:32.04 PSV.dial :mixloop @13:56:32.11 PTV.dial :PRVmixing @13:56:32.13 PRV.dial CBV @13:56:32.15
CBV.dial PRV @13:56:32.31 CTV.dial :air,avoiding: :intake @13:56:32.39 Loading lyfil puck from tube 3 into Collection Clamp @13:56:32.63 SC.reconfigure :tight @13:56:32.68 SC.seek 3 @13:56:36.21 SC.configure :limp @13:56:36.43 Elbow.reconfigure :tight @13:56:36.47 Elbow.seek 3 @13:56:38.40 Forearm.reconfigure :tight @13:56:38.45 Forearm.seek 3 + 450 counts @13:56:39.48 SE.reconfigure :pick @13:56:39.54 SE.seek :beamBreak @13:56:42.40 SE.configure :default @13:56:42.56 Elbow.seek 3 @13:56:42.75 Forearm.seek 3 + 300 counts @13:56:42.97 Forearm.reconfigure :loose @13:56:43.01 Hand.close @13:56:43.69 SE.seek :down @13:56:43.88
Forearm.seek :retract @13:56:44.88 Elbow.seek CC @13:56:46.21 SE.stop @13:56:46.95
Elbow.reconfigure :loose @13:56:47.05 Forearm.reconfigure :tight @13:56:47.10 Forearm.seek CC @13:56:48.37 CC.seek :closed @13:56:52.88 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.17 @13:56:52.89 Hand.open @13:56:53.61 Forearm.reconfigure :loose @13:56:53.66 Forearm.seek :retract @13:56:54.66 CTV.dial CSV,avoiding: :intake @13:56:56.01 CSV.dial CTV @13:56:56.35 PS.seek 2.5ml @13:57:22.92 CS.seek 4.499ml @13:57:37.86 delay 8 seconds @13:57:45.88 PSV.dial :air @13:57:46.49 delay 2 seconds @13:57:48.50 PSV.dial :waste2 @13:57:49.27 PS.seek 0ml @13:58:15.64 CBV.dial between PRV and waste1 @13:58:15.97 CSV.dial :air @13:58:16.44 CS.seek 10ml @13:58:31.26 CSV.dial CTV @13:58:31.72 CS.seek 0ml @13:58:58.10 CBV.dial :waste1 @13:58:58.42 delay 5 seconds @13:59:03.44 CTV.dial :air,avoiding: :intake @13:59:04.85 Storing lyfil puck in Collection Clamp to tube 2 @13:59:05.02 SC.reconfigure :tight @13:59:05.49 SC.seek 2 @13:59:09.12 SC.configure :limp @13:59:09.33 Elbow.reconfigure :tight @13:59:09.38 Elbow.seek 2 @13:59:11.18 Forearm.reconfigure :tight @13:59:11.23 Forearm.seek 2 + 450 counts @13:59:12.31 SE.reconfigure :pick @13:59:12.36 SE.seek :beamBreak @13:59:13.68 SE.configure :default @13:59:13.84 Elbow.reconfigure :limp @13:59:13.89 Forearm.reconfigure :loose @13:59:13.93 Forearm.seek 2 @13:59:14.21 Elbow.hold @13:59:14.27 Elbow.reconfigure :tight @13:59:14.37 SE.seek 27.08 Pucks below Top Plate @13:59:14.87 Forearm.force -20 @13:59:14.91 Forearm.seek 2 + 300 counts @13:59:15.22 Forearm.reconfigure :tight @13:59:15.27 Forearm.seek 2 + 450 counts @13:59:15.49 SE.seek 28.08 Pucks below Top Plate @13:59:16.06 Forearm.reconfigure :loose @13:59:16.11 Forearm.seek :retract @13:59:17.03 Elbow.seek CC @13:59:18.71 Elbow.reconfigure :loose @13:59:18.81 Forearm.seek CC @13:59:19.78 Hand.close @13:59:20.42 CC.seek :open @13:59:25.59 Forearm.seek :retract @13:59:26.55 Elbow.reconfigure :tight @13:59:26.60 Elbow.seek 2 @13:59:28.38 Elbow.reconfigure :limp @13:59:28.43 Forearm.seek 2 @13:59:29.40 Elbow.hold @13:59:29.47 Elbow.reconfigure :tight @13:59:29.52 Hand.open @13:59:30.30 Forearm.force -20 @13:59:30.34 Forearm.seek 2 + 303 counts @13:59:30.63 Forearm.reconfigure :tight @13:59:30.68 Forearm.seek 2 + 450 counts @13:59:30.94 SE.seek :down @13:59:31.72 SE.stop @13:59:32.07
Forearm.reconfigure :loose @13:59:32.11 Forearm.seek :retract @13:59:33.03 PSV.dial :air @13:59:33.79 PS.seek 2.5ml @14:00:00.14 delay 1 second @14:00:01.15 PSV.dial :mixloop @14:00:01.61 delay 1 second @14:00:02.62 PSV.dial :air @14:00:03.09 delay 1 second @14:00:04.11 PSV.dial :mixloop @14:00:04.58 delay 1 second @14:00:05.59 PSV.dial :air @14:00:06.06 delay 1 second @14:00:07.08 PS.seek 0ml @14:00:33.42 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @14:00:33.60 Elbow.seek FlushPuck::Garage @14:00:35.41 Elbow.reconfigure :loose @14:00:35.51 Forearm.seek :garage @14:00:36.56 Hand.close @14:00:37.67 Forearm.seek :retract @14:00:38.66 Elbow.reconfigure :tight @14:00:38.71 Elbow.seek PC @14:00:39.99 Elbow.reconfigure :loose @14:00:40.09 Forearm.reconfigure :tight @14:00:40.14 Forearm.seek PC @14:00:41.39 PC.seek :closed @14:00:46.01 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.59 @14:00:46.08 Hand.open @14:00:46.73 Forearm.reconfigure :loose @14:00:46.78 Forearm.seek :retract @14:00:47.79 PRV.dial :air @14:00:48.25 PSV.dial :mixloop @14:00:48.78 PS.seek 0.3ml @14:00:52.76 PSV.dial PSR @14:00:53.93 Pulling 0.4ml of PSR.air @14:00:53.94 PSR.select :air @14:00:53.97 PS.seek 0.7ml @14:00:58.83 delay 3 seconds @14:01:01.84 PSR.select :air @14:01:01.87 PS.seek 2.5ml @14:01:21.04 delay 3 seconds @14:01:24.06 PSV.dial :mixloop @14:01:25.22 PTV.dial :mixing @14:01:25.98 PBV.dial :waste2 @14:01:26.94 PS.seek 0ml @14:01:53.23 Garaging FlushPuck in Processing Clamp @14:01:53.30 Elbow.reconfigure :tight @14:01:53.35 Elbow.seek PC @14:01:53.46 Elbow.reconfigure :loose @14:01:54.01 Forearm.seek PC @14:01:54.98 Hand.close @14:01:55.55 PC.seek :open @14:02:00.26 Forearm.seek :retract @14:02:01.22 Elbow.reconfigure :tight @14:02:01.26 Elbow.seek :garage @14:02:02.65 Elbow.reconfigure :loose @14:02:02.75 Forearm.reconfigure :tight @14:02:02.79 Forearm.seek :garage @14:02:03.88 Hand.open @14:02:04.60 Forearm.reconfigure :loose @14:02:04.65 Forearm.seek :retract @14:02:05.71 Loading da puck from tube 3 into Processing Clamp @14:02:05.94 SC.reconfigure :tight @14:02:05.99 SC.seek 3 @14:02:09.57 SC.configure :limp @14:02:09.78 Elbow.reconfigure :tight @14:02:09.83 Elbow.seek 3 @14:02:11.71 Forearm.reconfigure :tight @14:02:11.76 Forearm.seek 3 + 450 counts @14:02:12.79 SE.reconfigure :pick @14:02:12.85 SE.seek :beamBreak @14:02:15.82 SE.configure :default @14:02:15.98 Elbow.seek 3 @14:02:16.16 Forearm.seek 3 + 300 counts @14:02:16.42 Forearm.reconfigure :loose @14:02:16.46 Hand.close @14:02:17.06 SE.seek :down @14:02:17.69
Forearm.seek :retract @14:02:18.73 Elbow.seek PC @14:02:19.66 SE.stop @14:02:21.52
Elbow.reconfigure :loose @14:02:21.67 Forearm.reconfigure :tight @14:02:21.72 Forearm.seek PC @14:02:22.90 PC.seek :closed @14:02:27.52 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.63 @14:02:27.53 Hand.open @14:02:28.22 Forearm.reconfigure :loose @14:02:28.27 Forearm.seek :retract @14:02:29.24 Heating Puck.from 3, :da in Processing Clamp to 28C for 2:00:00 @14:02:29.26 PH.seek 28C,5:00 @14:03:13.03 PH.hold 2:00:00,28C @14:03:13.08 delay 1:30 @14:04:43.13 PSR.select :air @14:04:43.15 PSV.dial PSR @14:04:44.20 Pulling 0.5ml of PSR.wash @14:04:44.21 PSR.select :wash @14:04:44.33 PS.seek 0.5ml @14:04:50.20 delay 5 seconds @14:04:55.22 PSR.select :air @14:04:55.34 PS.seek 2ml @14:05:11.51 PTV.dial :puck @14:05:12.26 PSV.dial PTV @14:05:13.02 PBV.dial :waste1 @14:05:14.43 PS.seek 1.5ml @14:05:20.32 delay 3 seconds @14:05:23.34 PBV.dial between PSV and waste1 @14:05:23.70 PS.seek 0ml @14:05:39.89 delay 5:00 @14:10:39.90 recoverPSRreagent08 @14:10:39.91 PTV.dial :puck @14:10:39.99 PBV.dial :waste1 @14:10:40.36 delay 30 seconds @14:11:10.39 PBV.dial between PSV and waste1 @14:11:10.77 PSR.select :air @14:11:10.78 PSV.dial PSR @14:11:11.54 PS.reconfigure :fast @14:11:11.59 PS.seek 2.5ml @14:11:35.47 PSV.dial PTV @14:11:36.24 delay 5 seconds @14:11:41.26 PSV.dial PSR @14:11:42.02 delay 5 seconds @14:11:47.04 PSV.dial PTV @14:11:47.80 delay 5 seconds @14:11:52.82 PSV.dial :air @14:11:53.59 PS.seek 0ml @14:12:16.78 PTV.dial :PRVmixing,via: PRV @14:12:18.11 PSV.dial :mixloop @14:12:18.58 PRV.dial :ab1 @14:12:19.71 PS.reconfigure :slow @14:12:19.82 PS.seek 0.15ml @14:12:23.44 delay 2 seconds @14:12:25.46 CBV.dial PRV @14:12:25.48
PRV.dial CBV @14:12:26.06 PS.seek 0.35ml @14:12:30.69 delay 2 seconds @14:12:32.71 CBV.dial between PRV and PSV @14:12:33.03 PRV.dial :air @14:12:33.49 PS.seek 0.9ml @14:12:45.19 PRV.dial :waste2 @14:12:45.93 PS.seek 0.5ml @14:12:54.66 addPMLReagent08 @14:12:54.67 PSV.dial :air @14:12:55.13 PS.reconfigure :default @14:12:55.18 PS.seek 2ml @14:13:11.28 PSV.dial :PML @14:13:11.75 PTV.dial :mixing @14:13:12.50 PBV.dial :waste1 @14:13:12.94 PS.seek 1.85ml @14:13:15.28 PBV.dial between PSV and waste1 @14:13:15.66 PS.seek 0ml @14:13:35.31 delay 2 seconds @14:13:37.33 Beginning samplePassC @14:13:37.34 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:13:37.53 Elbow.reconfigure :tight @14:13:37.58 Elbow.seek FlushPuck::Garage @14:13:38.92 Elbow.reconfigure :loose @14:13:39.02 Forearm.seek :garage @14:13:40.03 Hand.close @14:13:40.78 Forearm.seek :retract @14:13:41.77 Elbow.reconfigure :tight @14:13:41.82 Elbow.seek CC @14:13:44.73 Elbow.reconfigure :loose @14:13:44.83 Forearm.reconfigure :tight @14:13:44.88 Forearm.seek CC @14:13:46.58 CC.seek :closed @14:13:51.10 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.49 @14:13:51.17 Hand.open @14:13:51.85 Forearm.reconfigure :loose @14:13:51.89 Forearm.seek :retract @14:13:52.87 PSV.dial PSR @14:13:53.95 Pulling 1.5ml of PSR.flush @14:13:53.96 PSR.select :flush @14:13:54.08 PS.seek 1.5ml @14:14:10.22 delay 5 seconds @14:14:15.24 PSV.dial :waste1 @14:14:15.69 PS.seek 0ml @14:14:31.87 PSV.dial PSR @14:14:32.34 PSR.select :air @14:14:32.46 PS.seek 2.5ml @14:14:58.79 PRV.dial CBV,avoiding: :ab2 @14:14:58.81
CBV.dial PRV @14:15:00.44 PSV.dial :mixloop @14:15:01.48 CTV.dial :air,avoiding: :intake @14:15:01.56 PTV.dial :PRVmixing @14:15:02.33 PS.seek 0ml @14:15:28.68 PSV.dial :air @14:15:29.16 PS.seek 2.5ml @14:15:55.46 PSV.dial :mixloop @14:15:55.94 PS.seek 0ml @14:16:22.46 PSV.dial :air @14:16:22.94 PS.seek 2.5ml @14:16:49.46 PSV.dial :mixloop @14:16:49.94 PS.seek 0ml @14:17:16.43 Garaging FlushPuck in Collection Clamp @14:17:16.50 Elbow.reconfigure :tight @14:17:16.55 Elbow.seek CC @14:17:16.85 Elbow.reconfigure :loose @14:17:17.39 Forearm.seek CC @14:17:18.42 Hand.close @14:17:19.03 CC.seek :open @14:17:23.68 Forearm.seek :retract @14:17:24.72 Elbow.reconfigure :tight @14:17:24.77 Elbow.seek :garage @14:17:27.73 Elbow.reconfigure :loose @14:17:27.83 Forearm.reconfigure :tight @14:17:27.88 Forearm.seek :garage @14:17:28.89 Hand.open @14:17:29.59 Forearm.reconfigure :loose @14:17:29.64 Forearm.seek :retract @14:17:30.67 delayUntil "14:21:37.32" @14:17:30.70 Whole Cell Archive sampling 1000ml @14:17:30.72 Loading wcr puck from tube 3 into Collection Clamp @14:17:31.19 Elbow.reconfigure :tight @14:17:31.24 Elbow.seek 3 @14:17:33.20 Forearm.reconfigure :tight @14:17:33.25 Forearm.seek 3 + 450 counts @14:17:34.29 SE.reconfigure :pick @14:17:34.35 SE.seek :beamBreak @14:17:37.34 SE.configure :default @14:17:37.50 Elbow.seek 3 @14:17:37.69 Forearm.seek 3 + 300 counts @14:17:37.90 Forearm.reconfigure :loose @14:17:37.95 Hand.close @14:17:38.57 SE.seek :down @14:17:38.78 Forearm.seek :retract @14:17:39.75 Elbow.seek CC @14:17:41.33 SE.stop @14:17:41.36 Elbow.reconfigure :loose @14:17:41.50 Forearm.reconfigure :tight @14:17:41.55 Forearm.seek CC @14:17:42.88 CC.seek :closed @14:17:47.41 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.39 @14:17:47.42 Hand.open @14:17:48.09 Forearm.reconfigure :loose @14:17:48.14 Forearm.seek :retract @14:17:49.20 collectSample 1000ml w/bubble point of 35psi @14:17:49.22 Priming 7ml @14:17:49.23 Intake.select :close @14:17:49.24 Exhaust.select :close @14:17:49.25 SS.hold @14:17:49.47 Calibrating pressure sensors @14:17:49.48 CTV.dial :air @14:17:49.56 SSV.dial :spit,avoiding: :exhaust @14:17:49.65 SS.reconfigure :fast @14:17:49.70 SS.seek 5ml @14:17:52.33 SSV.dial CBV @14:17:52.36 CBV.dial SSV @14:17:53.75 delay 3.8 seconds @14:17:57.69 Sensor limits: Intake max 106.9psia; Outlet max 98.3psia @14:17:57.70 SSV.dial :spit @14:17:59.06 SS.seek 0ml @14:18:02.38 SSV.dial CBV @14:18:03.71 CTV.dial :intake @14:18:04.48 SSV.dial CBV @14:18:04.50 CBV.dial SSV @14:18:04.72 Allowing 40s for 7.0ml of remaining 7.0ml @14:18:04.74 Intake.select :open @14:18:06.90 Intake @14.8psia, vacuum from 9.3 to 12.3psi @14:18:06.92 SS.reconfigure :inhaling 2.20ml/s @14:18:06.97 SS.seek 7ml @14:18:13.50 Stroke completed -- waiting for pressure to equalize ... @14:18:13.51 SS.reconfigure :exhale @14:18:13.56 SS.hold @14:18:27.95 Intake.select :close @14:18:28.11 SSV.dial :exhaust @14:18:29.33 Exhaust.select :open @14:18:29.45 SS.seek 0ml @14:18:33.53 Exhaust.select :close @14:18:33.64 Sampling 1000.0ml @14:18:33.66 Intake.select :close @14:18:33.67 Exhaust.select :close @14:18:33.68 SS.reconfigure :default @14:18:33.72 SS.hold @14:18:33.94 CTV.dial :intake @14:18:34.17 SSV.dial CBV @14:18:34.19 CBV.dial SSV @14:18:35.02 Allowing 130s for 25.0ml of remaining 1000.0ml @14:18:35.03 Intake.select :open @14:18:37.20 Intake @15.8psia, vacuum from 10.0 to 13.0psi @14:18:37.22 SS.reconfigure :inhaling 2.20ml/s @14:18:37.27 SS.seek 25ml @14:18:44.39 Vacuum too high -- waiting up to 40 seconds @14:18:44.40 SS.reconfigure :exhale @14:18:44.46 SS.hold @14:18:50.48 Intake @17.3psia, vacuum from 10.0 to 13.0psi @14:18:50.50 SS.reconfigure :inhaling 1.76ml/s @14:18:50.55 SS.seek 25ml @14:18:53.98 Vacuum too high -- waiting up to 40 seconds @14:18:53.99 SS.reconfigure :exhale @14:18:54.05 SS.hold @14:18:57.07 Intake @17.6psia, vacuum from 10.0 to 13.0psi @14:18:57.09 SS.reconfigure :inhaling 1.41ml/s @14:18:57.14 SS.seek 25ml @14:19:00.88 Vacuum too high -- waiting up to 40 seconds @14:19:00.90 SS.reconfigure :exhale @14:19:00.95 SS.hold @14:19:04.07 Intake @18.3psia, vacuum from 10.0 to 13.0psi @14:19:04.09 SS.reconfigure :inhaling 1.13ml/s @14:19:04.14 SS.seek 25ml @14:19:07.95 Vacuum too high -- waiting up to 40 seconds @14:19:07.96 SS.reconfigure :exhale @14:19:08.02 SS.hold @14:19:10.13 Intake @17.9psia, vacuum from 10.0 to 13.0psi @14:19:10.15 SS.reconfigure :inhaling 0.91ml/s @14:19:10.65 SS.seek 25ml @14:19:14.03 Stroke completed -- waiting for pressure to equalize ... @14:19:14.04 SS.reconfigure :exhale @14:19:14.09 SS.hold @14:19:38.62 Intake.select :close @14:19:38.77 SSV.dial :exhaust @14:19:39.98 Exhaust.select :open @14:19:40.10 SS.seek 0ml @14:19:48.87 Exhaust.select :close @14:19:48.98 SSV.dial CBV @14:19:49.03 CBV.dial SSV @14:19:49.77 Allowing 130s for 25.0ml of remaining 975.0ml @14:19:49.78 Intake.select :open @14:19:51.95 Intake @16.1psia, vacuum from 10.0 to 13.0psi @14:19:51.97 SS.reconfigure :inhaling 0.91ml/s @14:19:52.02 SS.seek 25ml @14:20:22.11 Stroke completed -- waiting for pressure to equalize ... @14:20:22.12 SS.reconfigure :exhale @14:20:22.17 SS.hold @14:20:50.78 Intake.select :close @14:20:50.94 SSV.dial :exhaust @14:20:51.70 Exhaust.select :open @14:20:51.82 SS.seek 0ml @14:21:00.81 Exhaust.select :close @14:21:00.92 SSV.dial CBV @14:21:00.96 CBV.dial SSV @14:21:01.73 Allowing 130s for 25.0ml of remaining 950.0ml @14:21:01.75 Intake.select :open @14:21:03.91 Intake @16.3psia, vacuum from 10.0 to 13.0psi @14:21:03.93 SS.reconfigure :inhaling 0.91ml/s @14:21:03.98 SS.seek 25ml @14:21:33.68 Stroke completed -- waiting for pressure to equalize ... @14:21:33.70 SS.reconfigure :exhale @14:21:33.74 SS.hold @14:21:37.33 recoverPRVreagent08 @14:21:37.34 PTV.dial :mixing @14:21:38.10 PBV.dial :waste1 @14:21:38.49 delay 30 seconds @14:22:00.32 Intake.select :close @14:22:00.48 SSV.dial :exhaust @14:22:01.27 Exhaust.select :open @14:22:01.39 SS.seek 0ml @14:22:08.51 PBV.dial between PSV and waste1 @14:22:08.88 PSV.dial :air @14:22:09.36 PS.reconfigure :fast @14:22:09.40 PS.seek 2.5ml @14:22:10.43 Exhaust.select :close @14:22:10.55 SSV.dial CBV @14:22:10.57 CBV.dial SSV @14:22:11.36 Allowing 130s for 25.0ml of remaining 925.0ml @14:22:11.37 Intake.select :open @14:22:13.55 Intake @16.3psia, vacuum from 10.0 to 13.0psi @14:22:13.57 SS.reconfigure :inhaling 0.91ml/s @14:22:13.61 SS.seek 25ml @14:22:33.58 PSV.dial :mixloop @14:22:34.07 delay 2 seconds @14:22:36.09 PSV.dial :air @14:22:36.55 delay 2 seconds @14:22:38.57 PSV.dial :mixloop @14:22:39.04 delay 2 seconds @14:22:41.05 PSV.dial :air @14:22:41.53 PS.seek 0ml @14:22:43.03 Stroke completed -- waiting for pressure to equalize ... @14:22:43.04 SS.reconfigure :exhale @14:22:43.09 SS.hold @14:23:05.46 washCycleDA #1 of 2 @14:23:05.47 PSR.select :air @14:23:05.48 PSV.dial PSR @14:23:06.83 Pulling 0.5ml of PSR.wash @14:23:06.84 PSR.select :wash @14:23:06.96 PS.reconfigure :default @14:23:07.01 PS.seek 0.5ml @14:23:08.15 Intake.select :close @14:23:08.31 SSV.dial :exhaust @14:23:09.07 Exhaust.select :open @14:23:09.19 SS.seek 0ml @14:23:13.05 delay 5 seconds @14:23:18.00 Exhaust.select :close @14:23:18.07 PSR.select :air @14:23:18.13 SSV.dial CBV @14:23:18.18 CBV.dial SSV @14:23:18.26 PS.seek 2ml @14:23:18.92 Allowing 130s for 25.0ml of remaining 900.0ml @14:23:18.93 Intake.select :open @14:23:21.10 Intake @16.2psia, vacuum from 10.0 to 13.0psi @14:23:21.12 SS.reconfigure :inhaling 0.91ml/s @14:23:21.17 SS.seek 25ml @14:23:34.39 PTV.dial :puck @14:23:35.14 PSV.dial PTV @14:23:35.89 PBV.dial :waste1 @14:23:36.33 PS.seek 1.5ml @14:23:42.30 delay 3 seconds @14:23:45.32 PBV.dial between PSV and waste1 @14:23:45.70 PS.seek 0ml @14:23:51.06 Stroke completed -- waiting for pressure to equalize ... @14:23:51.07 SS.reconfigure :exhale @14:23:51.12 SS.hold @14:24:01.87 delay 2:00 @14:24:14.04 Intake.select :close @14:24:14.20 SSV.dial :exhaust @14:24:14.96 Exhaust.select :open @14:24:15.08 SS.seek 0ml @14:24:24.09 Exhaust.select :close @14:24:24.20 SSV.dial CBV @14:24:24.23 CBV.dial SSV @14:24:25.01 Allowing 130s for 25.0ml of remaining 875.0ml @14:24:25.02 Intake.select :open @14:24:27.19 Intake @16.2psia, vacuum from 10.0 to 13.0psi @14:24:27.21 SS.reconfigure :inhaling 0.91ml/s @14:24:27.26 SS.seek 25ml @14:24:56.88 Stroke completed -- waiting for pressure to equalize ... @14:24:56.89 SS.reconfigure :exhale @14:24:56.94 SS.hold @14:25:17.74 Intake.select :close @14:25:17.89 SSV.dial :exhaust @14:25:18.68 Exhaust.select :open @14:25:18.79 SS.seek 0ml @14:25:28.02 Exhaust.select :close @14:25:28.13 SSV.dial CBV @14:25:28.17 CBV.dial SSV @14:25:28.93 Allowing 130s for 25.0ml of remaining 850.0ml @14:25:28.95 Intake.select :open @14:25:31.11 Intake @16.2psia, vacuum from 10.0 to 13.0psi @14:25:31.13 SS.reconfigure :inhaling 0.91ml/s @14:25:31.18 SS.seek 25ml @14:26:00.15 Stroke completed -- waiting for pressure to equalize ... @14:26:00.16 SS.reconfigure :exhale @14:26:00.21 SS.hold @14:26:01.95 recoverPSRreagent08 @14:26:01.97 PTV.dial :puck @14:26:02.05 PBV.dial :waste1 @14:26:02.52 delay 30 seconds @14:26:20.99 Intake.select :close @14:26:21.15 SSV.dial :exhaust @14:26:21.93 Exhaust.select :open @14:26:22.05 SS.seek 0ml @14:26:31.07 Exhaust.select :close @14:26:31.18 SSV.dial CBV @14:26:31.22 CBV.dial SSV @14:26:31.98 Allowing 130s for 25.0ml of remaining 825.0ml @14:26:31.99 Intake.select :open @14:26:32.54 PBV.dial between PSV and waste1 @14:26:32.91 PSR.select :air @14:26:32.93 PSV.dial PSR @14:26:34.08 PS.reconfigure :fast @14:26:34.20 PS.seek 2.5ml @14:26:34.30 Intake @16.2psia, vacuum from 10.0 to 13.0psi @14:26:34.32 SS.reconfigure :inhaling 0.91ml/s @14:26:34.37 SS.seek 25ml @14:26:58.58 PSV.dial PTV @14:26:59.35 delay 5 seconds @14:27:00.00 Vacuum too high -- waiting up to 40 seconds @14:27:00.02 SS.reconfigure :exhale @14:27:00.07 SS.hold @14:27:04.36 PSV.dial PSR @14:27:05.13 delay 5 seconds @14:27:06.74 Intake @16.9psia, vacuum from 10.0 to 13.0psi @14:27:06.76 SS.reconfigure :inhaling 0.73ml/s @14:27:06.81 SS.seek 25ml @14:27:10.14 PSV.dial PTV @14:27:11.03 delay 5 seconds @14:27:11.74 Stroke completed -- waiting for pressure to equalize ... @14:27:11.75 SS.reconfigure :exhale @14:27:11.80 SS.hold @14:27:16.13 PSV.dial :air @14:27:16.93 PS.seek 0ml @14:27:24.67 Intake.select :close @14:27:24.83 SSV.dial :exhaust @14:27:25.61 Exhaust.select :open @14:27:25.73 SS.seek 0ml @14:27:34.79 Exhaust.select :close @14:27:34.90 SSV.dial CBV @14:27:34.92 CBV.dial SSV @14:27:35.76 Allowing 130s for 25.0ml of remaining 800.0ml @14:27:35.77 Intake.select :open @14:27:37.94 Intake @16.2psia, vacuum from 10.0 to 13.0psi @14:27:37.96 SS.reconfigure :inhaling 0.73ml/s @14:27:38.01 SS.seek 25ml @14:27:40.61 washCycleDA #2 of 2 @14:27:40.62 PSR.select :air @14:27:40.64 PSV.dial PSR @14:27:41.98 Pulling 0.5ml of PSR.wash @14:27:41.99 PSR.select :wash @14:27:42.11 PS.reconfigure :default @14:27:42.16 PS.seek 0.5ml @14:27:48.14 delay 5 seconds @14:27:53.16 PSR.select :air @14:27:53.28 PS.seek 2ml @14:28:09.48 PTV.dial :puck @14:28:09.56 PSV.dial PTV @14:28:10.31 PBV.dial :waste1 @14:28:10.89 PS.seek 1.5ml @14:28:13.69 Stroke completed -- waiting for pressure to equalize ... @14:28:13.70 SS.reconfigure :exhale @14:28:13.75 SS.hold @14:28:16.89 delay 3 seconds @14:28:19.91 PBV.dial between PSV and waste1 @14:28:20.30 PS.seek 0ml @14:28:28.14 Intake.select :close @14:28:28.30 SSV.dial :exhaust @14:28:29.08 Exhaust.select :open @14:28:29.20 SS.seek 0ml @14:28:36.45 delay 2:00 @14:28:37.97 Exhaust.select :close @14:28:38.08 SSV.dial CBV @14:28:38.10 CBV.dial SSV @14:28:39.35 Allowing 130s for 25.0ml of remaining 775.0ml @14:28:39.36 Intake.select :open @14:28:41.54 Intake @16.2psia, vacuum from 10.0 to 13.0psi @14:28:41.56 SS.reconfigure :inhaling 0.73ml/s @14:28:41.61 SS.seek 25ml @14:29:17.89 Stroke completed -- waiting for pressure to equalize ... @14:29:17.91 SS.reconfigure :exhale @14:29:17.95 SS.hold @14:29:34.28 Intake.select :close @14:29:34.44 SSV.dial :exhaust @14:29:35.22 Exhaust.select :open @14:29:35.34 SS.seek 0ml @14:29:44.78 Exhaust.select :close @14:29:44.89 SSV.dial CBV @14:29:44.94 CBV.dial SSV @14:29:45.68 Allowing 130s for 25.0ml of remaining 750.0ml @14:29:45.69 Intake.select :open @14:29:47.86 Intake @16.1psia, vacuum from 10.0 to 13.0psi @14:29:47.88 SS.reconfigure :inhaling 0.73ml/s @14:29:47.93 SS.seek 25ml @14:30:23.89 Stroke completed -- waiting for pressure to equalize ... @14:30:23.90 SS.reconfigure :exhale @14:30:23.95 SS.hold @14:30:36.47 recoverPSRreagent08 @14:30:36.48 PTV.dial :puck @14:30:36.60 PBV.dial :waste1 @14:30:37.03 delay 30 seconds @14:30:40.75 Intake.select :close @14:30:40.91 SSV.dial :exhaust @14:30:41.68 Exhaust.select :open @14:30:41.79 SS.seek 0ml @14:30:50.83 Exhaust.select :close @14:30:50.94 SSV.dial CBV @14:30:50.98 CBV.dial SSV @14:30:51.75 Allowing 130s for 25.0ml of remaining 725.0ml @14:30:51.77 Intake.select :open @14:30:53.94 Intake @16.0psia, vacuum from 10.0 to 13.0psi @14:30:53.95 SS.reconfigure :inhaling 0.73ml/s @14:30:54.00 SS.seek 25ml @14:31:07.04 PBV.dial between PSV and waste1 @14:31:07.42 PSR.select :air @14:31:07.44 PSV.dial PSR @14:31:08.21 PS.reconfigure :fast @14:31:08.25 PS.seek 2.5ml @14:31:29.86 Stroke completed -- waiting for pressure to equalize ... @14:31:29.87 SS.reconfigure :exhale @14:31:29.92 SS.hold @14:31:32.45 PSV.dial PTV @14:31:33.23 delay 5 seconds @14:31:38.25 PSV.dial PSR @14:31:39.41 delay 5 seconds @14:31:44.43 PSV.dial PTV @14:31:44.69 Intake.select :close @14:31:44.85 SSV.dial :exhaust @14:31:45.20 delay 5 seconds @14:31:45.63 Exhaust.select :open @14:31:45.75 SS.seek 0ml @14:31:50.22 PSV.dial :air @14:31:50.99 PS.seek 0ml @14:31:54.74 Exhaust.select :close @14:31:54.85 SSV.dial CBV @14:31:54.87 CBV.dial SSV @14:31:55.66 Allowing 130s for 25.0ml of remaining 700.0ml @14:31:55.68 Intake.select :open @14:31:57.85 Intake @16.1psia, vacuum from 10.0 to 13.0psi @14:31:57.87 SS.reconfigure :inhaling 0.73ml/s @14:31:57.92 SS.seek 25ml @14:32:14.70 getPMLReagent08 @14:32:14.71 PRV.dial :air @14:32:15.18 delay 5 seconds @14:32:20.20 PTV.dial :PRVmixing @14:32:21.54 delay 5 seconds @14:32:26.56 PRV.dial :ab2 @14:32:27.01 PTV.dial :PRVmixing @14:32:27.09 PSV.dial :mixloop @14:32:27.56 PS.reconfigure :default @14:32:27.61 PS.seek 0.3ml @14:32:31.51 delay 2 seconds @14:32:33.53 PRV.dial :air @14:32:33.81 Stroke completed -- waiting for pressure to equalize ... @14:32:33.82 SS.reconfigure :exhale @14:32:33.87 SS.hold @14:32:34.05 PS.seek 0.55ml @14:32:37.67 addPMLReagent08 @14:32:37.68 PSV.dial :air @14:32:38.14 PS.seek 2ml @14:32:52.70 Intake.select :close @14:32:52.86 SSV.dial :exhaust @14:32:53.64 Exhaust.select :open @14:32:53.73 PSV.dial :PML @14:32:53.78 SS.seek 0ml @14:32:54.20 PTV.dial :mixing @14:32:54.96 PBV.dial :waste1 @14:32:55.40 PS.seek 1.85ml @14:32:57.82 PBV.dial between PSV and waste1 @14:32:58.20 PS.seek 0ml @14:33:02.58 Exhaust.select :close @14:33:02.69 SSV.dial CBV @14:33:02.72 CBV.dial SSV @14:33:03.48 Allowing 130s for 25.0ml of remaining 675.0ml @14:33:03.50 Intake.select :open @14:33:06.05 Intake @16.0psia, vacuum from 10.0 to 13.0psi @14:33:06.14 SS.reconfigure :inhaling 0.73ml/s @14:33:06.19 SS.seek 25ml @14:33:17.98 delay 2 seconds @14:33:20.00 delay 8:00 @14:33:42.45 Stroke completed -- waiting for pressure to equalize ... @14:33:42.46 SS.reconfigure :exhale @14:33:42.51 SS.hold @14:34:00.92 Intake.select :close @14:34:01.08 SSV.dial :exhaust @14:34:01.84 Exhaust.select :open @14:34:01.96 SS.seek 0ml @14:34:11.04 Exhaust.select :close @14:34:11.62 SSV.dial CBV @14:34:11.67 CBV.dial SSV @14:34:12.42 Allowing 130s for 25.0ml of remaining 650.0ml @14:34:12.44 Intake.select :open @14:34:14.60 Intake @16.0psia, vacuum from 10.0 to 13.0psi @14:34:14.62 SS.reconfigure :inhaling 0.73ml/s @14:34:14.67 SS.seek 25ml @14:34:50.43 Stroke completed -- waiting for pressure to equalize ... @14:34:50.45 SS.reconfigure :exhale @14:34:50.49 SS.hold @14:35:11.37 Intake.select :close @14:35:11.53 SSV.dial :exhaust @14:35:12.31 Exhaust.select :open @14:35:12.43 SS.seek 0ml @14:35:21.26 Exhaust.select :close @14:35:21.37 SSV.dial CBV @14:35:21.40 CBV.dial SSV @14:35:22.20 Allowing 130s for 25.0ml of remaining 625.0ml @14:35:22.21 Intake.select :open @14:35:24.38 Intake @16.1psia, vacuum from 10.0 to 13.0psi @14:35:24.40 SS.reconfigure :inhaling 0.73ml/s @14:35:24.45 SS.seek 25ml @14:35:53.93 Vacuum too high -- waiting up to 40 seconds @14:35:53.94 SS.reconfigure :exhale @14:35:54.00 SS.hold @14:36:03.91 Intake @17.2psia, vacuum from 10.0 to 13.0psi @14:36:03.93 SS.reconfigure :inhaling 0.59ml/s @14:36:03.98 SS.seek 25ml @14:36:13.20 Stroke completed -- waiting for pressure to equalize ... @14:36:13.28 SS.reconfigure :exhale @14:36:13.33 SS.hold @14:36:24.48 Intake.select :close @14:36:24.64 SSV.dial :exhaust @14:36:25.42 Exhaust.select :open @14:36:25.54 SS.seek 0ml @14:36:34.57 Exhaust.select :close @14:36:34.68 SSV.dial CBV @14:36:34.70 CBV.dial SSV @14:36:35.51 Allowing 130s for 25.0ml of remaining 600.0ml @14:36:35.53 Intake.select :open @14:36:37.69 Intake @15.9psia, vacuum from 10.0 to 13.0psi @14:36:37.71 SS.reconfigure :inhaling 0.59ml/s @14:36:37.76 SS.seek 25ml @14:37:22.07 Stroke completed -- waiting for pressure to equalize ... @14:37:22.08 SS.reconfigure :exhale @14:37:22.13 SS.hold @14:37:40.80 Intake.select :close @14:37:40.96 SSV.dial :exhaust @14:37:41.74 Exhaust.select :open @14:37:41.86 SS.seek 0ml @14:37:50.87 Exhaust.select :close @14:37:50.98 SSV.dial CBV @14:37:51.02 CBV.dial SSV @14:37:51.79 Allowing 130s for 25.0ml of remaining 575.0ml @14:37:51.81 Intake.select :open @14:37:54.37 Intake @16.1psia, vacuum from 10.0 to 13.0psi @14:37:54.46 SS.reconfigure :inhaling 0.59ml/s @14:37:54.51 SS.seek 25ml @14:38:38.95 Stroke completed -- waiting for pressure to equalize ... @14:38:38.96 SS.reconfigure :exhale @14:38:39.01 SS.hold @14:38:59.44 Intake.select :close @14:38:59.60 SSV.dial :exhaust @14:39:00.42 Exhaust.select :open @14:39:00.54 SS.seek 0ml @14:39:09.93 Exhaust.select :close @14:39:10.05 SSV.dial CBV @14:39:10.07 CBV.dial SSV @14:39:10.87 Allowing 130s for 25.0ml of remaining 550.0ml @14:39:10.88 Intake.select :open @14:39:13.05 Intake @16.0psia, vacuum from 10.0 to 13.0psi @14:39:13.07 SS.reconfigure :inhaling 0.59ml/s @14:39:13.12 SS.seek 25ml @14:39:57.91 Stroke completed -- waiting for pressure to equalize ... @14:39:57.92 SS.reconfigure :exhale @14:39:57.97 SS.hold @14:40:20.51 Intake.select :close @14:40:20.67 SSV.dial :exhaust @14:40:21.46 Exhaust.select :open @14:40:21.58 SS.seek 0ml @14:40:30.65 Exhaust.select :close @14:40:30.76 SSV.dial CBV @14:40:30.78 CBV.dial SSV @14:40:31.60 Allowing 130s for 25.0ml of remaining 525.0ml @14:40:31.61 Intake.select :open @14:40:33.78 Intake @16.0psia, vacuum from 10.0 to 13.0psi @14:40:33.80 SS.reconfigure :inhaling 0.59ml/s @14:40:33.85 SS.seek 25ml @14:41:17.99 Stroke completed -- waiting for pressure to equalize ... @14:41:18.00 SS.reconfigure :exhale @14:41:18.05 SS.hold @14:41:20.07 recoverPRVreagent08 @14:41:20.08 PTV.dial :mixing @14:41:20.17 PBV.dial :waste1 @14:41:20.55 delay 30 seconds @14:41:44.68 Intake.select :close @14:41:44.84 SSV.dial :exhaust @14:41:45.61 Exhaust.select :open @14:41:45.72 SS.seek 0ml @14:41:50.58 PBV.dial between PSV and waste1 @14:41:50.95 PSV.dial :air @14:41:51.43 PS.reconfigure :fast @14:41:51.48 PS.seek 2.5ml @14:41:54.52 Exhaust.select :close @14:41:54.64 SSV.dial CBV @14:41:54.66 CBV.dial SSV @14:41:55.46 Allowing 130s for 25.0ml of remaining 500.0ml @14:41:55.47 Intake.select :open @14:41:57.64 Intake @15.9psia, vacuum from 10.0 to 13.0psi @14:41:57.66 SS.reconfigure :inhaling 0.59ml/s @14:41:57.71 SS.seek 25ml @14:42:15.53 PSV.dial :mixloop @14:42:16.02 delay 2 seconds @14:42:18.03 PSV.dial :air @14:42:18.50 delay 2 seconds @14:42:20.52 PSV.dial :mixloop @14:42:20.98 delay 2 seconds @14:42:23.00 PSV.dial :air @14:42:23.47 PS.seek 0ml @14:42:40.45 Vacuum too high -- waiting up to 40 seconds @14:42:40.47 SS.reconfigure :exhale @14:42:40.52 SS.hold @14:42:46.94 washCycleDA #1 of 3 @14:42:46.95 PSR.select :air @14:42:46.97 PSV.dial PSR @14:42:48.33 Pulling 0.5ml of PSR.wash @14:42:48.35 PSR.select :wash @14:42:48.47 PS.reconfigure :default @14:42:48.51 PS.seek 0.5ml @14:42:54.50 delay 5 seconds @14:42:55.30 Intake @17.2psia, vacuum from 10.0 to 13.0psi @14:42:55.31 SS.reconfigure :inhaling 0.47ml/s @14:42:55.36 SS.seek 25ml @14:42:58.47 Stroke completed -- waiting for pressure to equalize ... @14:42:58.48 SS.reconfigure :exhale @14:42:58.53 SS.hold @14:42:59.51 PSR.select :air @14:42:59.63 PS.seek 2ml @14:43:11.41 Intake.select :close @14:43:11.57 SSV.dial :exhaust @14:43:12.35 Exhaust.select :open @14:43:12.47 SS.seek 0ml @14:43:15.84 PTV.dial :puck @14:43:16.59 PSV.dial PTV @14:43:17.36 PBV.dial :waste1 @14:43:17.79 PS.seek 1.5ml @14:43:21.50 Exhaust.select :close @14:43:21.61 SSV.dial CBV @14:43:21.65 CBV.dial SSV @14:43:22.43 Allowing 130s for 25.0ml of remaining 475.0ml @14:43:22.44 Intake.select :open @14:43:23.81 delay 3 seconds @14:43:24.61 Intake @15.9psia, vacuum from 10.0 to 13.0psi @14:43:24.63 SS.reconfigure :inhaling 0.47ml/s @14:43:24.68 SS.seek 25ml @14:43:26.83 PBV.dial between PSV and waste1 @14:43:27.21 PS.seek 0ml @14:43:43.40 delay 2:00 @14:44:19.80 Stroke completed -- waiting for pressure to equalize ... @14:44:19.81 SS.reconfigure :exhale @14:44:19.86 SS.hold @14:44:42.80 Intake.select :close @14:44:42.96 SSV.dial :exhaust @14:44:43.74 Exhaust.select :open @14:44:43.85 SS.seek 0ml @14:44:52.92 Exhaust.select :close @14:44:53.03 SSV.dial CBV @14:44:53.05 CBV.dial SSV @14:44:54.35 Allowing 130s for 25.0ml of remaining 450.0ml @14:44:54.36 Intake.select :open @14:44:56.53 Intake @16.0psia, vacuum from 10.0 to 13.0psi @14:44:56.55 SS.reconfigure :inhaling 0.47ml/s @14:44:56.60 SS.seek 25ml @14:45:43.43 recoverPSRreagent08 @14:45:43.44 PTV.dial :puck @14:45:43.62 PBV.dial :waste1 @14:45:44.00 delay 30 seconds @14:45:51.70 Stroke completed -- waiting for pressure to equalize ... @14:45:51.72 SS.reconfigure :exhale @14:45:51.76 SS.hold @14:46:14.02 PBV.dial between PSV and waste1 @14:46:14.39 PSR.select :air @14:46:14.41 PSV.dial PSR @14:46:15.19 PS.reconfigure :fast @14:46:15.24 PS.seek 2.5ml @14:46:18.32 Intake.select :close @14:46:18.48 SSV.dial :exhaust @14:46:19.27 Exhaust.select :open @14:46:19.39 SS.seek 0ml @14:46:28.26 Exhaust.select :close @14:46:28.37 SSV.dial CBV @14:46:28.41 CBV.dial SSV @14:46:29.17 Allowing 130s for 25.0ml of remaining 425.0ml @14:46:29.19 Intake.select :open @14:46:31.35 Intake @16.0psia, vacuum from 10.0 to 13.0psi @14:46:31.37 SS.reconfigure :inhaling 0.47ml/s @14:46:31.42 SS.seek 25ml @14:46:39.40 PSV.dial PTV @14:46:40.29 delay 5 seconds @14:46:45.31 PSV.dial PSR @14:46:46.06 delay 5 seconds @14:46:51.08 PSV.dial PTV @14:46:51.84 delay 5 seconds @14:46:56.86 PSV.dial :air @14:46:57.77 PS.seek 0ml @14:47:21.21 washCycleDA #2 of 3 @14:47:21.22 PSR.select :air @14:47:21.24 PSV.dial PSR @14:47:22.71 Pulling 0.5ml of PSR.wash @14:47:22.72 PSR.select :wash @14:47:22.84 PS.reconfigure :default @14:47:22.89 PS.seek 0.5ml @14:47:26.88 Stroke completed -- waiting for pressure to equalize ... @14:47:26.89 SS.reconfigure :exhale @14:47:26.94 SS.hold @14:47:28.80 delay 5 seconds @14:47:33.86 PSR.select :air @14:47:34.04 PS.seek 2ml @14:47:50.18 PTV.dial :puck @14:47:50.26 PSV.dial PTV @14:47:51.13 PBV.dial :waste1 @14:47:51.57 PS.seek 1.5ml @14:47:54.20 Intake.select :close @14:47:54.36 SSV.dial :exhaust @14:47:55.15 Exhaust.select :open @14:47:55.26 SS.seek 0ml @14:47:57.56 delay 3 seconds @14:48:00.57 PBV.dial between PSV and waste1 @14:48:00.97 PS.seek 0ml @14:48:04.14 Exhaust.select :close @14:48:04.72 SSV.dial CBV @14:48:04.77 CBV.dial SSV @14:48:05.51 Allowing 130s for 25.0ml of remaining 400.0ml @14:48:05.53 Intake.select :open @14:48:07.70 Intake @16.1psia, vacuum from 10.0 to 13.0psi @14:48:07.72 SS.reconfigure :inhaling 0.47ml/s @14:48:07.77 SS.seek 25ml @14:48:17.09 delay 2:00 @14:48:50.82 Vacuum too high -- waiting up to 40 seconds @14:48:50.84 SS.reconfigure :exhale @14:48:50.89 SS.hold @14:49:05.40 Intake @17.1psia, vacuum from 10.0 to 13.0psi @14:49:05.42 SS.reconfigure :inhaling 0.38ml/s @14:49:05.47 SS.seek 25ml @14:49:21.15 Stroke completed -- waiting for pressure to equalize ... @14:49:21.16 SS.reconfigure :exhale @14:49:21.62 SS.hold @14:49:44.15 Intake.select :close @14:49:44.31 SSV.dial :exhaust @14:49:45.07 Exhaust.select :open @14:49:45.19 SS.seek 0ml @14:49:54.24 Exhaust.select :close @14:49:54.78 SSV.dial CBV @14:49:54.80 CBV.dial SSV @14:49:55.61 Allowing 130s for 25.0ml of remaining 375.0ml @14:49:55.62 Intake.select :open @14:49:57.79 Intake @15.9psia, vacuum from 10.0 to 13.0psi @14:49:57.81 SS.reconfigure :inhaling 0.38ml/s @14:49:57.86 SS.seek 25ml @14:50:17.11 recoverPSRreagent08 @14:50:17.12 PTV.dial :puck @14:50:17.19 PBV.dial :waste1 @14:50:17.57 delay 30 seconds @14:50:47.60 PBV.dial between PSV and waste1 @14:50:47.97 PSR.select :air @14:50:47.99 PSV.dial PSR @14:50:48.75 PS.reconfigure :fast @14:50:48.80 PS.seek 2.5ml @14:51:06.68 Stroke completed -- waiting for pressure to equalize ... @14:51:06.69 SS.reconfigure :exhale @14:51:06.74 SS.hold @14:51:12.96 PSV.dial PTV @14:51:13.72 delay 5 seconds @14:51:18.74 PSV.dial PSR @14:51:19.97 delay 5 seconds @14:51:24.99 PSV.dial PTV @14:51:25.75 delay 5 seconds @14:51:30.77 PSV.dial :air @14:51:31.54 PS.seek 0ml @14:51:35.39 Intake.select :close @14:51:35.55 SSV.dial :exhaust @14:51:36.32 Exhaust.select :open @14:51:36.44 SS.seek 0ml @14:51:45.48 Exhaust.select :close @14:51:45.59 SSV.dial CBV @14:51:45.61 CBV.dial SSV @14:51:46.43 Allowing 130s for 25.0ml of remaining 350.0ml @14:51:46.45 Intake.select :open @14:51:48.61 Intake @16.0psia, vacuum from 10.0 to 13.0psi @14:51:48.63 SS.reconfigure :inhaling 0.38ml/s @14:51:48.68 SS.seek 25ml @14:51:55.15 washCycleDA #3 of 3 @14:51:55.16 PSR.select :air @14:51:55.18 PSV.dial PSR @14:51:56.52 Pulling 0.5ml of PSR.wash @14:51:56.53 PSR.select :wash @14:51:56.64 PS.reconfigure :default @14:51:56.69 PS.seek 0.5ml @14:52:02.68 delay 5 seconds @14:52:07.70 PSR.select :air @14:52:07.81 PS.seek 2ml @14:52:23.92 PTV.dial :puck @14:52:24.00 PSV.dial PTV @14:52:24.75 PBV.dial :waste1 @14:52:25.19 PS.seek 1.5ml @14:52:31.11 delay 3 seconds @14:52:34.13 PBV.dial between PSV and waste1 @14:52:34.51 PS.seek 0ml @14:52:50.71 delay 2:00 @14:52:57.07 Stroke completed -- waiting for pressure to equalize ... @14:52:57.56 SS.reconfigure :exhale @14:52:57.60 SS.hold @14:53:30.77 Intake.select :close @14:53:30.93 SSV.dial :exhaust @14:53:31.72 Exhaust.select :open @14:53:31.84 SS.seek 0ml @14:53:40.91 Exhaust.select :close @14:53:41.02 SSV.dial CBV @14:53:41.06 CBV.dial SSV @14:53:41.82 Allowing 130s for 25.0ml of remaining 325.0ml @14:53:41.83 Intake.select :open @14:53:44.00 Intake @16.1psia, vacuum from 10.0 to 13.0psi @14:53:44.02 SS.reconfigure :inhaling 0.38ml/s @14:53:44.07 SS.seek 25ml @14:54:50.73 recoverPSRreagent08 @14:54:50.74 PTV.dial :puck @14:54:50.81 PBV.dial :waste1 @14:54:51.19 delay 30 seconds @14:54:52.64 Stroke completed -- waiting for pressure to equalize ... @14:54:52.66 SS.reconfigure :exhale @14:54:52.71 SS.hold @14:55:21.21 PBV.dial between PSV and waste1 @14:55:21.58 PSR.select :air @14:55:21.60 PSV.dial PSR @14:55:22.37 PS.reconfigure :fast @14:55:22.42 PS.seek 2.5ml @14:55:29.57 Intake.select :close @14:55:29.73 SSV.dial :exhaust @14:55:30.51 Exhaust.select :open @14:55:30.63 SS.seek 0ml @14:55:39.74 Exhaust.select :close @14:55:39.85 SSV.dial CBV @14:55:39.88 CBV.dial SSV @14:55:40.66 Allowing 130s for 25.0ml of remaining 300.0ml @14:55:40.68 Intake.select :open @14:55:42.84 Intake @16.1psia, vacuum from 10.0 to 13.0psi @14:55:42.86 SS.reconfigure :inhaling 0.38ml/s @14:55:42.91 SS.seek 25ml @14:55:46.64 PSV.dial PTV @14:55:47.53 delay 5 seconds @14:55:52.55 PSV.dial PSR @14:55:53.31 delay 5 seconds @14:55:58.33 PSV.dial PTV @14:55:59.09 delay 5 seconds @14:56:04.11 PSV.dial :air @14:56:04.94 PS.seek 0ml @14:56:28.30 Gate.power :camera,:ON @14:56:28.31 Mixing DA Substrates @14:56:28.32 PSR.select :air @14:56:28.34 PSV.dial PSR @14:56:29.75 Pulling 0.25ml of PSR.sub1 @14:56:29.77 PSR.select :sub1 @14:56:29.98 PS.reconfigure :default @14:56:30.09 PS.seek 0.25ml @14:56:33.68 delay 5 seconds @14:56:38.70 Pulling 0.25ml of PSR.sub2 @14:56:38.72 PSR.select :sub2 @14:56:38.83 PS.seek 0.5ml @14:56:42.26 delay 5 seconds @14:56:44.22 Vacuum too high -- waiting up to 40 seconds @14:56:44.23 SS.reconfigure :exhale @14:56:44.29 SS.hold @14:56:47.28 PSR.select :air @14:56:47.39 PS.seek 2ml @14:57:03.48 PTV.dial :puck @14:57:03.56 PSV.dial PTV @14:57:04.34 PBV.dial :waste1 @14:57:04.92 PS.seek 1.5ml @14:57:04.93 Intake @17.3psia, vacuum from 10.0 to 13.0psi @14:57:04.97 SS.reconfigure :inhaling 0.31ml/s @14:57:05.06 SS.seek 25ml @14:57:10.98 delay 3 seconds @14:57:13.92 Stroke completed -- waiting for pressure to equalize ... @14:57:13.94 SS.reconfigure :exhale @14:57:13.98 SS.hold @14:57:14.06 PBV.dial between PSV and waste1 @14:57:14.44 PS.seek 0ml @14:57:30.61 delay 10 seconds @14:57:36.94 Intake.select :close @14:57:37.10 SSV.dial :exhaust @14:57:37.88 Exhaust.select :open @14:57:38.00 SS.seek 0ml @14:57:40.63 recoverPSRreagent08 @14:57:40.64 PTV.dial :puck @14:57:40.72 PBV.dial :waste1 @14:57:41.09 delay 30 seconds @14:57:47.06 Exhaust.select :close @14:57:47.17 SSV.dial CBV @14:57:47.19 CBV.dial SSV @14:57:48.03 Allowing 130s for 25.0ml of remaining 275.0ml @14:57:48.05 Intake.select :open @14:57:50.21 Intake @16.1psia, vacuum from 10.0 to 13.0psi @14:57:50.23 SS.reconfigure :inhaling 0.31ml/s @14:57:50.28 SS.seek 25ml @14:58:11.12 PBV.dial between PSV and waste1 @14:58:11.50 PSR.select :air @14:58:11.52 PSV.dial PSR @14:58:12.30 PS.reconfigure :fast @14:58:12.35 PS.seek 2.5ml @14:58:36.85 PSV.dial PTV @14:58:37.62 delay 5 seconds @14:58:42.64 PSV.dial PSR @14:58:43.42 delay 5 seconds @14:58:48.44 PSV.dial PTV @14:58:49.22 delay 5 seconds @14:58:54.24 PSV.dial :air @14:58:55.02 PS.seek 0ml @14:59:19.11 Additional air clear @14:59:19.12 PSR.select :air @14:59:19.13 PSV.dial PSR @14:59:19.78 Stroke completed -- waiting for pressure to equalize ... @14:59:19.79 SS.reconfigure :exhale @14:59:19.84 SS.hold @14:59:20.50 Pulling 2ml of PSR.air @14:59:20.51 PSR.select :air @14:59:20.53 PS.reconfigure :default @14:59:20.58 PS.seek 2ml @14:59:41.89 delay 5 seconds @14:59:46.91 PTV.dial :puck @14:59:47.00 PSV.dial PTV @14:59:47.78 PBV.dial :waste1 @14:59:48.21 PS.seek 1.5ml @14:59:54.20 delay 3 seconds @14:59:56.89 Intake.select :close @14:59:57.05 SSV.dial :exhaust @14:59:57.21 PBV.dial between PSV and waste1 @14:59:57.59 delay 1 second @14:59:57.84 Exhaust.select :open @14:59:57.96 SS.seek 0ml @14:59:58.61 PSV.dial :waste1 @14:59:59.69 PS.seek 0ml @15:00:06.78 Exhaust.select :close @15:00:06.89 SSV.dial CBV @15:00:06.94 CBV.dial SSV @15:00:07.72 Allowing 130s for 25.0ml of remaining 250.0ml @15:00:07.73 Intake.select :open @15:00:09.90 Intake @16.0psia, vacuum from 10.0 to 13.0psi @15:00:09.92 SS.reconfigure :inhaling 0.31ml/s @15:00:09.97 SS.seek 25ml @15:00:15.94 Processing Puck Heater at 28.1C before imaging @15:00:15.95 PH.coast @15:00:16.06 Imaging da puck in Processing Clamp while storing it to tube 2 @15:00:16.24 SC.reconfigure :tight @15:00:16.29 SC.seek 2 @15:00:19.90 SC.configure :limp @15:00:20.12 Elbow.reconfigure :tight @15:00:20.17 Elbow.seek 2 @15:00:21.97 Forearm.reconfigure :tight @15:00:22.02 Forearm.seek 2 + 450 counts @15:00:23.02 SE.reconfigure :pick @15:00:23.08 SE.seek :beamBreak @15:00:24.34 SE.configure :default @15:00:24.50 Elbow.reconfigure :limp @15:00:24.55 Forearm.reconfigure :loose @15:00:24.60 Forearm.seek 2 @15:00:24.85 Elbow.hold @15:00:24.92 Elbow.reconfigure :tight @15:00:25.02 SE.seek 28.08 Pucks below Top Plate @15:00:25.98 Forearm.force -20 @15:00:26.03 Forearm.seek 2 + 314 counts @15:00:26.32 Forearm.reconfigure :tight @15:00:26.37 Forearm.seek 2 + 450 counts @15:00:26.56 SE.seek 29.08 Pucks below Top Plate @15:00:27.13 Forearm.reconfigure :loose @15:00:27.18 Forearm.seek :retract @15:00:28.12 Elbow.seek PC @15:00:30.68 Elbow.reconfigure :loose @15:00:30.78 Forearm.seek PC @15:00:31.79 Hand.close @15:00:32.51 PC.seek :open @15:00:37.30 Forearm.seek :retract @15:00:38.33 Elbow.reconfigure :tight @15:00:38.38 Elbow.seek 2 @15:00:41.00 Forearm.reconfigure :tight @15:00:41.05 Forearm.seek 2 + 450 counts @15:00:42.01 Hand.open @15:00:42.68 SE.seek 28.73 Pucks below Top Plate @15:00:42.96 SE.reconfigure :seal @15:00:43.02 SE.seek 27.76 Pucks below Top Plate @15:00:43.55 Lights, camera, ACTION! @15:00:43.58 PuckCamera: snap -bin=2,2 -auto /var/log/esp/da23nov1515h875ml.tif @15:01:03.07 Waiting >20 seconds for da to relinquish FlushPuck @15:01:07.23 SX HX9/16 (USB:03): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 20.354 seconds /var/log/esp/da23nov1515h875ml.tif: TIFF Upload Complete @15:01:07.25 PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da23nov1515h875ml15s.tif @15:01:22.87 SX HX9/16 (USB:03): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15 seconds /var/log/esp/midres/da23nov1515h875ml15s.tif: TIFF Upload Complete @15:01:22.89 PuckCamera: snap -bin=2,2 20.0 /var/log/esp/midres/da23nov1515h875ml20s.tif @15:01:40.15 Stroke completed -- waiting for pressure to equalize ... @15:01:40.17 SS.reconfigure :exhale @15:01:40.22 SS.hold @15:01:43.59 SX HX9/16 (USB:03): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 20 seconds /var/log/esp/midres/da23nov1515h875ml20s.tif: TIFF Upload Complete @15:01:43.60 Gate.power :camera,:OFF @15:01:43.62 DAfans.email "20.354 sec auto-exposure",Subject: "da23nov1515h875ml images!" @15:01:44.04 Elbow.seek 2 @15:01:44.43 Forearm.seek 2 + 450 counts @15:01:44.51 SE.reconfigure :gently @15:01:44.56 SE.seek 28.48 Pucks below Top Plate @15:01:45.25 Elbow.reconfigure :limp @15:01:45.86 Forearm.reconfigure :loose @15:01:45.93 Forearm.seek 2 @15:01:46.26 Elbow.hold @15:01:46.33 Elbow.reconfigure :tight @15:01:46.38 SE.reconfigure :default @15:01:46.43 SE.seek 28.67 Pucks below Top Plate @15:01:47.01 Forearm.force -20 @15:01:47.08 Forearm.seek 2 + 292 counts @15:01:47.40 Forearm.reconfigure :tight @15:01:47.45 Forearm.seek 2 + 450 counts @15:01:47.74 SE.seek :down @15:01:48.49 SE.stop @15:01:48.94 Forearm.reconfigure :loose @15:01:49.56 Forearm.seek :retract @15:01:50.57 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @15:01:50.76 Elbow.seek FlushPuck::Garage @15:01:52.79 Elbow.reconfigure :loose @15:01:52.89 Forearm.seek :garage @15:01:53.85 Hand.close @15:01:54.61 Forearm.seek :retract @15:01:55.65 Elbow.reconfigure :tight @15:01:55.70 Elbow.seek PC @15:01:56.94 Elbow.reconfigure :loose @15:01:57.04 Forearm.reconfigure :tight @15:01:57.09 Forearm.seek PC @15:01:58.33 PC.seek :closed @15:02:02.95 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.59 @15:02:03.02 Hand.open @15:02:03.76 Forearm.reconfigure :loose @15:02:03.81 Forearm.seek :retract @15:02:04.84 PSR.select :air @15:02:04.86 PSV.dial PSR @15:02:05.32 Pulling 0.5ml of PSR.flush @15:02:05.33 PSR.select :flush @15:02:05.45 PS.seek 0.5ml @15:02:11.48 delay 5 seconds @15:02:16.50 PSR.select :air @15:02:16.76 PS.seek 2.5ml @15:02:17.75 SS.reconfigure :default @15:02:17.86 SS.hold @15:02:18.00 Intake.select :close @15:02:18.25 ShallowSampler::Clogged in WCR -- Filtering too Slowly after 775.0ml @15:02:18.28 SSV.dial :exhaust @15:02:19.11 Exhaust.select :open @15:02:19.28 SS.reconfigure :exhale @15:02:19.38 SS.seek 0ml @15:02:28.20 Exhaust.select :close @15:02:28.36 Sampled 775.0ml @15:02:28.40 Drying puck before fixing for 5:00 @15:02:28.43 CBV.dial :waste2b @15:02:29.24 delay 1:00 @15:02:38.13 delay 1 second @15:02:39.17 PRV.dial :waste2,avoiding: :ab2 @15:02:41.13 PTV.dial :PRVmixing @15:02:42.49 PSV.dial :mixloop @15:02:43.59 PS.seek :empty @15:02:44.35 upsync @15:03:10.07 PSR.select :air @15:03:10.09 PSV.dial PSR @15:03:11.15 Pulling 0.5ml of PSR.flush @15:03:11.16 PSR.select :flush @15:03:11.28 PS.seek 0.5ml @15:03:17.29 delay 5 seconds @15:03:22.31 PSR.select :air @15:03:22.43 PS.seek 2.5ml @15:03:29.27 CSV.dial :air @15:03:29.72 CS.reconfigure :fast @15:03:29.78 CS.seek 10ml @15:03:43.76 delay 1 second @15:03:44.78 PBV.dial :waste1 @15:03:45.15 PTV.dial :mixing @15:03:45.91 PSV.dial :mixloop @15:03:46.98 PS.seek :empty @15:03:54.83 Pumping wcr puck from tube 3 up to 35psi... @15:03:54.85 CBV.dial SSV @15:03:56.13 CBV.dial between CSV and waste2b @15:03:57.11 CSV.dial between air and CTV @15:03:57.43 CS.seek 7.079ml @15:04:04.87 CTV.dial CSV,avoiding: :air @15:04:04.91 CSV.dial CTV @15:04:05.71 CS.reconfigure :pressurize @15:04:05.76 CS.seek 0ml @15:04:13.35 PSV.dial PSR @15:04:14.40 PSR.select :flush @15:04:14.52 PS.seek 1ml @15:04:19.16 CBV.dial :waste2b @15:04:19.50 CS.reconfigure :default @15:04:19.55 CS.seek 0ml @15:04:25.52 PSR.select :air @15:04:25.55 delay 1:00 @15:04:25.67 PS.seek 2.5ml @15:04:41.88 PSV.dial PTV @15:04:42.65 PTV.dial :puck @15:04:43.40 PBV.dial :waste1 @15:04:43.48 PS.seek 0ml @15:05:09.82 PSV.dial PSR @15:05:10.58 PSR.select :flush @15:05:10.71 PS.seek 0.5ml @15:05:16.97 PSR.select :air @15:05:17.09 PS.seek 2.5ml @15:05:25.58 CTV.dial :air,avoiding: :intake @15:05:26.91 Fix #1 of 2 (with RNAlater) for 2:30 @15:05:26.92 CSV.dial CSR @15:05:27.36 Pulling 2ml of CSR.RNAlater @15:05:27.37 CSR.select :RNAlater @15:05:27.49 CS.seek 2ml @15:05:33.39 delay 5 seconds @15:05:38.42 PSV.dial PTV @15:05:38.46 CSR.select :air @15:05:39.02 CS.reconfigure :fast @15:05:39.08 CS.seek 10ml @15:05:39.30 PBV.dial PSV @15:05:39.89 PS.seek 0ml @15:05:58.76 Pumping wcr puck from tube 3 up to 35psi... @15:05:58.78 CBV.dial SSV @15:06:00.03 CBV.dial between CSV and waste2b @15:06:01.01 CSV.dial between air and CTV @15:06:01.61 CS.seek 7.079ml @15:06:06.16 PSV.dial PBV @15:06:06.62 PS.seek 2.5ml @15:06:09.11 CTV.dial CSV,avoiding: :air @15:06:09.13 CSV.dial CTV @15:06:10.55 CS.reconfigure :pressurize @15:06:10.60 CS.seek 0ml @15:06:28.44 delay 2:30 @15:06:33.00 PSV.dial :waste1 @15:06:33.77 PS.seek 0ml @15:07:00.19 PSV.dial PSR @15:07:00.66 PSR.select :air @15:07:00.69 PS.seek 2.5ml @15:07:27.09 PSV.dial PTV @15:07:27.85 PBV.dial PSV @15:07:28.04 PS.seek 0ml @15:07:54.59 PSV.dial PBV @15:07:55.07 PS.seek 2.5ml @15:08:21.44 PSV.dial :waste1 @15:08:22.20 PS.seek 0ml @15:08:48.69 PSV.dial PSR @15:08:49.16 PSR.select :flush @15:08:49.28 PS.seek 1ml @15:08:58.46 CBV.dial :waste2b @15:08:58.80 CS.reconfigure :default @15:08:58.85 CS.seek 0ml @15:09:00.35 delay 1:00 @15:09:00.50 PSR.select :air @15:09:00.62 PS.seek 2.5ml @15:09:17.00 PSV.dial PTV @15:09:17.75 PTV.dial :puck @15:09:17.83 PBV.dial :waste1 @15:09:18.82 PS.seek 0ml @15:09:45.15 PSV.dial PSR @15:09:45.92 PSR.select :flush @15:09:46.05 PS.seek 0.5ml @15:09:51.97 PSR.select :air @15:09:52.08 PS.seek 2.5ml @15:10:00.38 CTV.dial :air,avoiding: :intake @15:10:01.72 Fix #2 of 2 (with RNAlater) for 2:30 @15:10:01.73 CSV.dial CSR @15:10:02.18 Pulling 2ml of CSR.RNAlater @15:10:02.19 CSR.select :RNAlater @15:10:02.31 CS.seek 2ml @15:10:08.51 delay 5 seconds @15:10:13.34 PSV.dial PTV @15:10:13.52 CSR.select :air @15:10:13.65 CS.reconfigure :fast @15:10:13.70 CS.seek 10ml @15:10:14.11 PBV.dial PSV @15:10:14.68 PS.seek 0ml @15:10:33.51 Pumping wcr puck from tube 3 up to 35psi... @15:10:33.53 CBV.dial SSV @15:10:34.80 CBV.dial between CSV and waste2b @15:10:35.77 CSV.dial between air and CTV @15:10:36.37 CS.seek 7.017ml @15:10:41.03 PSV.dial PBV @15:10:41.51 PS.seek 2.5ml @15:10:43.95 CTV.dial CSV,avoiding: :air @15:10:43.98 CSV.dial CTV @15:10:45.37 CS.reconfigure :pressurize @15:10:45.43 CS.seek 0ml @15:10:59.47 delay 2:30 @15:11:07.87 PSV.dial :waste1 @15:11:08.63 PS.seek 0ml @15:11:34.96 PSV.dial PSR @15:11:35.43 PSR.select :air @15:11:35.46 PS.seek 2.5ml @15:12:01.84 PSV.dial PTV @15:12:02.58 PBV.dial PSV @15:12:02.66 PS.seek 0ml @15:12:28.96 PSV.dial PBV @15:12:29.88 PS.seek 2.5ml @15:12:56.31 PSV.dial :waste1 @15:12:57.07 PS.seek 0ml @15:13:23.50 Garaging FlushPuck in Processing Clamp @15:13:23.57 Elbow.reconfigure :tight @15:13:23.62 Elbow.seek PC @15:13:23.75 Elbow.reconfigure :loose @15:13:23.85 Forearm.seek PC @15:13:24.80 Hand.close @15:13:25.36 PC.seek :open @15:13:29.49 CBV.dial :waste2b @15:13:29.84 CS.reconfigure :default @15:13:29.89 CS.seek 0ml @15:13:30.11 Forearm.seek :retract @15:13:31.07 Elbow.reconfigure :tight @15:13:31.12 Elbow.seek :garage @15:13:32.46 Elbow.reconfigure :loose @15:13:32.57 Forearm.reconfigure :tight @15:13:32.62 Forearm.seek :garage @15:13:33.70 Hand.open @15:13:34.42 Forearm.reconfigure :loose @15:13:34.47 Forearm.seek :retract @15:13:34.97 delay 1:00 @15:14:35.00 CTV.dial :air,avoiding: :intake @15:14:36.36 Storing wcr puck in Collection Clamp to tube 2 @15:14:36.77 Elbow.reconfigure :tight @15:14:36.82 Elbow.seek 2 @15:14:38.67 Forearm.reconfigure :tight @15:14:38.72 Forearm.seek 2 + 450 counts @15:14:39.82 SE.reconfigure :pick @15:14:40.32 SE.seek :beamBreak @15:14:41.58 SE.configure :default @15:14:41.75 Elbow.reconfigure :limp @15:14:41.80 Forearm.reconfigure :loose @15:14:41.85 Forearm.seek 2 @15:14:42.11 Elbow.hold @15:14:42.18 Elbow.reconfigure :tight @15:14:42.29 SE.seek 29.08 Pucks below Top Plate @15:14:42.77 Forearm.force -20 @15:14:42.81 Forearm.seek 2 + 292 counts @15:14:43.12 Forearm.reconfigure :tight @15:14:43.17 Forearm.seek 2 + 450 counts @15:14:43.37 SE.seek 30.08 Pucks below Top Plate @15:14:43.95 Forearm.reconfigure :loose @15:14:43.99 Forearm.seek :retract @15:14:44.93 Elbow.seek CC @15:14:46.57 Elbow.reconfigure :loose @15:14:46.67 Forearm.seek CC @15:14:47.62 Hand.close @15:14:48.24 CC.seek :open @15:14:53.02 Forearm.seek :retract @15:14:54.04 Elbow.reconfigure :tight @15:14:54.09 Elbow.seek 2 @15:14:55.84 Elbow.reconfigure :limp @15:14:55.89 Forearm.seek 2 @15:14:57.36 Elbow.hold @15:14:57.43 Elbow.reconfigure :tight @15:14:57.48 Hand.open @15:14:58.23 Forearm.force -20 @15:14:58.28 Forearm.seek 2 + 319 counts @15:14:58.59 Forearm.reconfigure :tight @15:14:58.65 Forearm.seek 2 + 450 counts @15:14:58.91 SE.seek :down @15:14:59.57 SE.stop @15:15:00.11 Forearm.reconfigure :loose @15:15:00.16 Forearm.seek :retract @15:15:01.08 Starting finalPurge @15:15:01.09 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @15:15:01.28 Elbow.seek FlushPuck::Garage @15:15:03.03 Elbow.reconfigure :loose @15:15:03.13 Forearm.seek :garage @15:15:04.09 Hand.close @15:15:04.85 Forearm.seek :retract @15:15:05.86 Elbow.reconfigure :tight @15:15:05.91 Elbow.seek CC @15:15:08.88 Elbow.reconfigure :loose @15:15:08.97 Forearm.reconfigure :tight @15:15:09.02 Forearm.seek CC @15:15:10.23 CC.seek :closed @15:15:14.76 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.31 @15:15:14.83 Hand.open @15:15:15.55 Forearm.reconfigure :loose @15:15:15.59 Forearm.seek :retract @15:15:16.56 Flushing the Collection Valve::Manifold @15:15:16.69 CSV.dial CSR @15:15:17.14 Pulling 4.999ml of CSR.flush @15:15:17.15 CSR.select :flush @15:15:17.27 CS.seek 5ml @15:15:31.06 delay 5 seconds @15:15:36.08 CSR.select :air @15:15:36.25 CS.reconfigure :fast @15:15:36.30 CS.seek 7.5ml @15:15:43.13 CSV.dial :waste @15:15:44.43 CS.reconfigure :default @15:15:44.48 CS.seek 0ml @15:16:04.47 Flushing lower manifold's waste1 port @15:16:04.60 CSV.dial :flush @15:16:05.04 CS.reconfigure :fast @15:16:05.09 CS.seek 5ml @15:16:17.81 delay 5 seconds @15:16:22.83 CSV.dial :air @15:16:23.45 CS.seek 7ml @15:16:29.10 CSV.dial CTV @15:16:29.12
CTV.dial CSV @15:16:30.50 CBV.dial :waste1 @15:16:31.27 CS.seek 0ml @15:16:48.00 Flushing lower manifold's waste2 port @15:16:48.12 CSV.dial :flush @15:16:48.87 CS.seek 5ml @15:17:01.66 delay 5 seconds @15:17:06.68 CSV.dial :air @15:17:07.19 CS.seek 7ml @15:17:12.69 CSV.dial CTV @15:17:12.71
CTV.dial CSV @15:17:13.17 CBV.dial :waste2 @15:17:14.24 CS.seek 0ml @15:17:30.91 Flushing lower manifold's waste2b port @15:17:31.03 CSV.dial :flush @15:17:31.77 CS.seek 5ml @15:17:44.47 delay 5 seconds @15:17:49.94 CSV.dial :air @15:17:50.46 CS.seek 7ml @15:17:56.22 CSV.dial CTV @15:17:56.25
CTV.dial CSV @15:17:56.70 CBV.dial :waste2b @15:17:57.76 CS.seek 0ml @15:18:14.31 Disinfecting external Intake with kill @15:18:14.44 CSV.dial CSR @15:18:15.00 Pulling 8ml of CSR.kill @15:18:15.01 CSR.select :kill @15:18:15.12 CS.seek 8ml @15:18:35.00 delay 5 seconds @15:18:40.02 CSR.select :air @15:18:40.14 CS.seek 10ml @15:18:45.76 CSV.dial CBV @15:18:45.78
CBV.dial CSV @15:18:46.84 CTV.dial :intake @15:18:47.62 Intake.select :open @15:18:47.74 CS.seek 2ml @15:19:06.65 Intake.select :close @15:19:06.76 CTV.dial :air @15:19:07.50 CS.reconfigure :default @15:19:07.55 CS.seek 0ml @15:19:13.47 CSV.dial CSR @15:19:14.48 Pulling 5ml of CSR.flush @15:19:14.49 CSR.select :flush @15:19:14.61 CS.seek 5ml @15:19:28.44 delay 5 seconds @15:19:33.46 CSR.select :air @15:19:33.57 CS.seek 10ml @15:19:47.15 CSV.dial CBV @15:19:48.18 CS.seek 0ml @15:20:14.62 Garaging FlushPuck in Collection Clamp @15:20:14.69 Elbow.reconfigure :tight @15:20:14.74 Elbow.seek CC @15:20:15.01 Elbow.reconfigure :loose @15:20:15.11 Forearm.seek CC @15:20:16.15 Hand.close @15:20:16.73 CC.seek :open @15:20:21.39 Forearm.seek :retract @15:20:22.34 Elbow.reconfigure :tight @15:20:22.39 Elbow.seek :garage @15:20:25.29 Elbow.reconfigure :loose @15:20:25.38 Forearm.reconfigure :tight @15:20:25.43 Forearm.seek :garage @15:20:26.82 Hand.open @15:20:27.54 Forearm.reconfigure :loose @15:20:27.59 Forearm.seek :retract @15:20:28.58 Completed [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] begun 12:42:19 @15:20:28.63 Asleep after completing DA_WCR_111523 assays @15:20:28.65 GoodNews.email "{1 => 33,\n 2 => 31,\n 3 => 2,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@15:11:28, 30% flow, 17.0C, 25% humidity, 15.2psia, 13.671V, 0.496A, 14.159Ah, 6.78W\nSampled 775.0ml",Subject: "Asleep after completing DA_WCR_111523 assays" @15:20:32.77 upsync + ESPwhich DA_WCR_112023.rb + fn=DA_WCR_112023.rb + IFS=: + set /home/esp/esp2/mission . + fullPath=/home/esp/esp2/mission/DA_WCR_112023.rb + [ -f /home/esp/esp2/mission/DA_WCR_112023.rb ] + realpath /home/esp/esp2/mission/DA_WCR_112023.rb + return 0 + scriptPath=/home/esp/esp2local/mission/DA_WCR_112023.rb + exec ruby /home/esp/esp2/lib/esp.rb /home/esp/esp2local/mission/DA_WCR_112023.rb @15:52:22.89UTC20-Nov-23
ESP2 v3.42 I2C Gateway v3.20 for core bus @15:52:25.58 Configuration for short puck jake 1/11/21 by brent@mbari.org /home/esp/esp2/type/shallow/jake/configure.rb @15:52:29.20 Polling Can every 10:00 @15:52:29.27 Cleanly exited at 15:49:35UTC20-Nov-23 @15:52:29.31 Loading /home/esp/esp2local/mission/DA_WCR_112023.rb mission @15:52:29.37 Preparing to sleep until + 00:00:05 @15:52:29.39 GoodNews.email "Starting DA_WCR_112023 w/pucks in tube 3\n{1 => 33,\n 2 => 31,\n 3 => 2,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@15:52:29, 30% flow, 16.8C, 26% humidity, 15.2psia, 13.720V, 0.327A, 48.284Ah, 4.48W",Subject: "DA_WCR_112023 begun" @15:52:29.43
Asleep til + 00:00:05 @15:52:29.47 Continuing at 15:52 @15:52:29.71 Gate.power :core,:OFF @15:52:29.73 Gate.power :raw,:OFF @15:52:29.74 Shutdown @15:52:32.09 GoodNews.email "Then, in tube 3: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_112023.rb:14\n{1 => 33,\n 2 => 31,\n 3 => 2,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@15:52:29, 30% flow, 16.8C, 26% humidity, 15.2psia, 13.720V, 0.327A, 48.284Ah, 4.48W",Subject: "Asleep until 15:52" @15:52:33.46 upsync @15:52:34.35
Powering core bus @15:52:35.96 upsync @15:52:40.19
Gate.power :raw,:ON @15:52:40.89 CS.configure :default @15:52:40.94 PS.configure :default @15:52:40.99 SS.configure :default @15:52:41.03 CC.configure :default @15:52:41.08 PC.configure :default @15:52:41.15 Forearm.configure :loose @15:52:41.20 Elbow.configure :loose @15:52:41.27 SC.configure :loose @15:52:41.31 SE.configure :default @15:52:41.36 CH.configure :default @15:52:41.39 PH.configure :default @15:52:41.43 All dwarf controllers running firmware version 3.85 @15:52:41.54 Valve.reset @15:52:41.74 Intake.select :close @15:52:41.79 Exhaust.select :close @15:52:41.84 CTV.dial :air @15:52:41.95 PSV.dial :air @15:52:41.98 CSV.dial :waste1 @15:52:42.12
Forearm.home.seek :retract @15:52:42.48 SSV.dial :spit,avoiding: :exhaust @15:52:42.89 Hand.close @15:52:42.93
Elbow.reconfigure :loose @15:52:43.00 Elbow.jog 1500 @15:52:43.09 PS.home.seek 0ml @15:52:43.42
Elbow.reconfigure :loose @15:52:43.46 Elbow.home.seek :clear @15:52:44.45 CS.reconfigure :fast @15:52:44.49 SS.home.seek 0ml @15:52:44.58 CS.home.seek 0ml @15:52:46.75
SE.home.seek :down @15:52:47.37 SC.reconfigure :tight @15:52:47.42 SC.home.seek :initial @15:52:51.44 PS.stop @15:53:00.10
SC.configure :limp @15:53:00.15 Forearm.seek :retract @15:53:00.22 Elbow.seek :hammer @15:53:01.39 Forearm.reconfigure :tight @15:53:01.43 Forearm.seek retracted @15:53:01.82 PC.home.seek :open @15:53:02.22
CC.home.seek :open @15:53:09.52 Notice.email "At 15:53:09 began in tube 3: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_112023.rb:14\n{1 => 33,\n 2 => 31,\n 3 => 2,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@15:53:09, 30% flow, 16.7C, 26% humidity, 15.2psia, 13.715V, 0.278A, 48.288Ah, 3.82W",Subject: "Started DA_WCR_112023 phase initialPurge; da [1000, 1000] #da and wsr are run simultanelously" @15:53:09.55
Phase [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] @15:53:09.56 Starting initialPurge @15:53:09.57 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @15:53:09.65 Hand.open @15:53:10.43 Elbow.reconfigure :tight @15:53:10.48 Elbow.seek FlushPuck::Garage @15:53:12.43 Elbow.reconfigure :loose @15:53:12.54 Forearm.reconfigure :loose @15:53:12.59 Forearm.seek :garage @15:53:13.50 upsync @15:53:13.54
Hand.close @15:53:14.28 Forearm.seek :retract @15:53:15.26 Elbow.reconfigure :tight @15:53:15.32 Elbow.seek CC @15:53:18.37 Elbow.reconfigure :loose @15:53:18.47 Forearm.reconfigure :tight @15:53:18.52 Forearm.seek CC @15:53:19.74 CC.seek :closed @15:53:24.30 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 1.01 @15:53:24.33 Puck::Warning in MAIN -- Retaining claim on Arm because Hand is likely holding a puck! @15:53:24.34 Slide::Error in MAIN -- No Puck Clamped in Collection Clamp @15:53:24.35 Waiting to Resume from Checkpoint "No Puck Clamped in Collection Clamp" @15:53:24.37 BadNews.email "No Puck Clamped in Collection Clamp",Subject: "Slide::Error in MAIN" @15:53:28.22 upsync @15:54:34.09 Session Begun @15:54:43.41 -> abort! 'mission failed' @15:54:43.43 CmdServer::Client::Error in dave -- Aborting Mission: mission failed @15:54:43.49 BadNews.email "mission failed",Subject: "DA_WCR_112023 ABORTED!" # @15:54:47.28 upsync @15:54:47.34 Storage::Warning in MAIN -- Exiting with FlushPuck in Hand @15:54:47.40 Session Ended + ESPwhich DA_WCR_112223.rb + fn=DA_WCR_112223.rb + IFS=: + set /home/esp/esp2/mission . + fullPath=/home/esp/esp2/mission/DA_WCR_112223.rb + [ -f /home/esp/esp2/mission/DA_WCR_112223.rb ] + realpath /home/esp/esp2/mission/DA_WCR_112223.rb + return 0 + scriptPath=/home/esp/esp2local/mission/DA_WCR_112223.rb + exec ruby /home/esp/esp2/lib/esp.rb /home/esp/esp2local/mission/DA_WCR_112223.rb @19:15:12.56UTC22-Nov-23
ESP2 v3.42 I2C Gateway v3.20 for core bus @19:15:15.23 Configuration for short puck jake 1/11/21 by brent@mbari.org /home/esp/esp2/type/shallow/jake/configure.rb @19:15:18.92 Polling Can every 10:00 @19:15:18.99 Cleanly exited at 19:11:22UTC22-Nov-23 @19:15:19.03 Loading /home/esp/esp2local/mission/DA_WCR_112223.rb mission @19:15:19.05 RuntimeError in MAIN -- Mission's startTube should be 3, not 4 @19:15:19.07 Waiting to Resume from Checkpoint "Loading /home/esp/esp2local/mission/DA_WCR_112223.rb mission" @19:15:19.09 BadNews.email "Mission's startTube should be 3, not 4",Subject: "RuntimeError in MAIN" @19:15:23.52 upsync @19:19:24.74 Session Begun @19:19:34.03 -> abort! 'mission failed' @19:19:34.05 CmdServer::Client::Error in dave -- Aborting Mission: mission failed @19:19:34.09 BadNews.email "mission failed",Subject: "DA_WCR_112223 ABORTED!" # @19:19:38.09 upsync @19:19:38.17 Session Ended + ESPwhich DA_WCR_112223.rb + fn=DA_WCR_112223.rb + IFS=: + set /home/esp/esp2/mission . + fullPath=/home/esp/esp2/mission/DA_WCR_112223.rb + [ -f /home/esp/esp2/mission/DA_WCR_112223.rb ] + realpath /home/esp/esp2/mission/DA_WCR_112223.rb + return 0 + scriptPath=/home/esp/esp2local/mission/DA_WCR_112223.rb + exec ruby /home/esp/esp2/lib/esp.rb /home/esp/esp2local/mission/DA_WCR_112223.rb @19:35:40.51UTC22-Nov-23
ESP2 v3.42 I2C Gateway v3.20 for core bus @19:35:43.16 Configuration for short puck jake 1/11/21 by brent@mbari.org /home/esp/esp2/type/shallow/jake/configure.rb @19:35:46.82 Polling Can every 10:00 @19:35:47.01 Loading /home/esp/esp2local/mission/DA_WCR_112223.rb mission @19:35:47.07 GoodNews.email "Starting DA_WCR_112223 w/pucks in tube 3\n{}\nCTD data unavailable\nCan@19:35:46, 31% flow, 16.1C, 27% humidity, 15.1psia, 13.739V, 0.266A, 63.914Ah, 3.66W",Subject: "DA_WCR_112223 begun" @19:35:47.12
Preparing to sleep until + 00:00:05 @19:35:47.15 Asleep til + 00:00:05 @19:35:47.17 Continuing at 19:35 @19:35:47.45 Gate.power :core,:OFF @19:35:47.48 Gate.power :raw,:OFF @19:35:47.49 Shutdown @19:35:49.64 GoodNews.email "Then, in tube 3: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_112223.rb:14\n{1 => 33,\n 2 => 31,\n 3 => 2,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@19:35:47, 31% flow, 16.1C, 27% humidity, 15.1psia, 13.739V, 0.272A, 63.914Ah, 3.74W",Subject: "Asleep until 19:35" @19:35:51.01 upsync @19:35:52.05
Powering core bus @19:35:53.52 upsync @19:35:58.03
Gate.power :raw,:ON @19:35:58.72 CS.configure :default @19:35:58.76 PS.configure :default @19:35:58.81 SS.configure :default @19:35:58.86 CC.configure :default @19:35:58.91 PC.configure :default @19:35:58.98 Forearm.configure :loose @19:35:59.02 Elbow.configure :loose @19:35:59.09 SC.configure :loose @19:35:59.14 SE.configure :default @19:35:59.18 CH.configure :default @19:35:59.22 PH.configure :default @19:35:59.26 All dwarf controllers running firmware version 3.85 @19:35:59.36 Valve.reset @19:35:59.67 Intake.select :close @19:35:59.68 Exhaust.select :close @19:35:59.69 CTV.dial :air @19:35:59.77 PSV.dial :air @19:35:59.90 CSV.dial :waste1 @19:35:59.93
Forearm.home.seek :retract @19:36:00.05 PS.home.seek 0ml @19:36:00.13 SSV.dial :spit,avoiding: :exhaust @19:36:00.30 CS.reconfigure :fast @19:36:00.34 SS.home.seek 0ml @19:36:00.44 CS.home.seek 0ml @19:36:00.68 Hand.close @19:36:00.70
Elbow.reconfigure :loose @19:36:00.80 Elbow.jog 1500 @19:36:01.38 Elbow.reconfigure :loose @19:36:01.43 Elbow.home.seek :clear @19:36:05.92 SE.home.seek :down @19:36:06.52 SC.reconfigure :tight @19:36:06.57 SC.home.seek :initial @19:36:08.36 PS.stop @19:36:34.85
SC.configure :limp @19:36:34.91 Forearm.seek :retract @19:36:34.97 Elbow.seek :hammer @19:36:35.99 Forearm.reconfigure :tight @19:36:36.05 Forearm.seek retracted @19:36:36.32 PC.home.seek :open @19:36:36.33
CC.home.seek :open @19:36:39.72 Phase [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] @19:36:39.74 Notice.email "At 19:36:39 began in tube 3: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_112223.rb:14\n{1 => 33,\n 2 => 31,\n 3 => 2,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@19:36:39, 31% flow, 16.1C, 27% humidity, 15.1psia, 13.730V, 0.296A, 63.919Ah, 4.07W",Subject: "Started DA_WCR_112223 phase initialPurge; da [1000, 1000] #da and wsr are run simultanelously" @19:36:39.77
Starting initialPurge @19:36:39.78 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @19:36:39.84 Hand.open @19:36:40.62 Elbow.reconfigure :tight @19:36:40.67 Elbow.seek FlushPuck::Garage @19:36:42.61 Elbow.reconfigure :loose @19:36:42.72 Forearm.reconfigure :loose @19:36:42.78 Forearm.seek :garage @19:36:43.66 upsync @19:36:43.78
Hand.close @19:36:44.48 Forearm.seek :retract @19:36:45.53 Elbow.reconfigure :tight @19:36:45.58 Elbow.seek CC @19:36:48.54 Elbow.reconfigure :loose @19:36:48.65 Forearm.reconfigure :tight @19:36:48.70 Forearm.seek CC @19:36:50.01 CC.seek :closed @19:36:54.54 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.41 @19:36:54.60 Hand.open @19:36:55.30 Forearm.reconfigure :loose @19:36:55.34 Forearm.seek :retract @19:36:56.30 Flushing the Collection Valve::Manifold @19:36:56.43 CSV.dial CSR @19:36:57.73 Pulling 5ml of CSR.flush @19:36:57.74 CSR.select :flush @19:36:57.86 CS.reconfigure :default @19:36:57.91 CS.seek 5ml @19:37:11.91 delay 5 seconds @19:37:16.92 CSR.select :air @19:37:17.09 CS.reconfigure :fast @19:37:17.14 CS.seek 7.5ml @19:37:23.94 CSV.dial :waste @19:37:25.24 CS.reconfigure :default @19:37:25.28 CS.seek 0ml @19:37:45.22 Purging external Intake @19:37:45.34 CSV.dial :flush @19:37:45.78 CS.reconfigure :fast @19:37:45.84 CS.seek 10ml @19:38:10.34 delay 5 seconds @19:38:15.36 CSV.dial CBV @19:38:15.39
CBV.dial CSV @19:38:16.15 CTV.dial :intake @19:38:16.95 Intake.select :open @19:38:17.06 CS.seek 0ml @19:38:40.84 Intake.select :close @19:38:40.95 CTV.dial :air @19:38:41.72 Flushing upper manifold @19:38:41.84 CSV.dial :flush @19:38:42.58 CS.seek 5ml @19:38:55.31 delay 5 seconds @19:39:00.33 CSV.dial :air @19:39:00.84 CS.seek 7.001ml @19:39:06.59 CSV.dial CBV @19:39:06.61
CBV.dial CSV @19:39:07.65 CTV.dial :air @19:39:07.67 CS.seek 0ml @19:39:24.47 delay 7 seconds @19:39:31.49 CS.seek 3ml @19:39:39.62 CSV.dial :waste @19:39:40.08 CS.seek 0ml @19:39:47.92 CSV.dial :flush @19:39:48.38 CS.seek 3ml @19:39:56.43 delay 5 seconds @19:40:01.45 CSV.dial :air @19:40:01.95 CS.seek 5.001ml @19:40:07.71 CSV.dial :air @19:40:07.80 CS.seek 0ml @19:40:19.96 delay 7 seconds @19:40:26.99 CS.seek 3ml @19:40:35.02 CSV.dial :waste @19:40:35.77 CS.seek 0ml @19:40:43.47 Garaging FlushPuck in Collection Clamp @19:40:43.54 Elbow.reconfigure :tight @19:40:43.58 Elbow.seek CC @19:40:43.88 Elbow.reconfigure :loose @19:40:43.98 Forearm.seek CC @19:40:45.01 Hand.close @19:40:45.61 CC.seek :open @19:40:50.63 Forearm.seek :retract @19:40:51.66 Elbow.reconfigure :tight @19:40:51.71 Elbow.seek :garage @19:40:54.63 Elbow.reconfigure :loose @19:40:54.73 Forearm.reconfigure :tight @19:40:54.78 Forearm.seek :garage @19:40:55.85 Hand.open @19:40:56.54 Forearm.reconfigure :loose @19:40:56.59 Forearm.seek :retract @19:40:57.58 da sampling at most 1000ml, wcr at most 1000ml @19:40:57.62 Loading sh1 puck from tube 3 into Collection Clamp @19:40:57.63 DAfans.email "Started da in tube 3 at 19:40:57UTC22-Nov-23\n{1 => 33,\n 2 => 31,\n 3 => 2,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@19:36:39, 31% flow, 16.1C, 27% humidity, 15.1psia, 13.730V, 0.296A, 63.919Ah, 4.07W",Subject: "da sampling at most 1000ml, wcr at most 1000ml" @19:40:57.90
SC.reconfigure :tight @19:40:57.97 SC.seek 3 @19:41:01.41 upsync @19:41:01.56
SC.configure :limp @19:41:01.79 Elbow.reconfigure :tight @19:41:01.84 Elbow.seek 3 @19:41:03.81 Forearm.reconfigure :tight @19:41:03.86 Forearm.seek 3 + 450 counts @19:41:04.93 SE.reconfigure :pick @19:41:04.98 SE.seek :beamBreak @19:41:08.05 SE.configure :default @19:41:08.21 Elbow.seek 3 @19:41:08.39 Forearm.seek 3 + 300 counts @19:41:08.69 Forearm.reconfigure :loose @19:41:08.73 Hand.close @19:41:09.37 SE.seek :down @19:41:09.58
Forearm.seek :retract @19:41:10.91 Elbow.seek CC @19:41:12.08 SE.stop @19:41:12.57
Elbow.reconfigure :loose @19:41:12.67 Forearm.reconfigure :tight @19:41:12.72 Forearm.seek CC @19:41:13.94 CC.seek :closed @19:41:18.46 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.30 @19:41:18.47 Hand.open @19:41:19.17 Forearm.reconfigure :loose @19:41:19.21 Forearm.seek :retract @19:41:20.22 collectSample 1000ml w/bubble point of 24psi @19:41:20.23 Priming 7ml @19:41:20.24 Intake.select :close @19:41:20.25 Exhaust.select :close @19:41:20.26 SS.hold @19:41:20.48 Calibrating pressure and temperature sensors @19:41:20.50 CTV.dial :air @19:41:20.52 SSV.dial :spit,avoiding: :exhaust @19:41:20.61 SS.reconfigure :fast @19:41:20.65 SS.seek 5ml @19:41:23.52 SSV.dial CBV @19:41:23.57
CBV.dial SSV @19:41:24.90 delay 3.8 seconds @19:41:28.83 Sensor limits: Intake max 106.2psia; Outlet max 98.0psia SSV.dial :spit @19:41:30.17 SS.seek 0ml @19:41:33.18 SSV.dial CBV @19:41:34.51 CTV.dial :intake @19:41:35.30 SSV.dial CBV @19:41:35.33
CBV.dial SSV @19:41:35.54 Allowing 40s for 7.0ml of remaining 7.0ml @19:41:35.55 Intake.select :open @19:41:37.71 Intake @14.2psia, vacuum from 8.7 to 11.7psi @19:41:37.72 SS.reconfigure :inhaling 2.20ml/s @19:41:37.77 SS.seek 7ml @19:41:44.30 Stroke completed -- waiting for pressure to equalize ... @19:41:44.31 SS.reconfigure :exhale @19:41:44.36 SS.hold @19:41:52.99 Intake.select :close @19:41:53.14 SSV.dial :exhaust @19:41:53.93 Exhaust.select :open @19:41:54.04 SS.seek 0ml @19:41:58.15 Exhaust.select :close @19:41:58.26 Sampling 1000.0ml @19:41:58.27 Intake.select :close @19:41:58.28 Exhaust.select :close @19:41:58.29 SS.reconfigure :default @19:41:58.33 SS.hold @19:41:58.53 CTV.dial :intake @19:41:58.70 SSV.dial CBV @19:41:58.72
CBV.dial SSV @19:41:59.52 Allowing 130s for 25.0ml of remaining 1000.0ml @19:41:59.53 Intake.select :open @19:42:01.70 Intake @15.0psia, vacuum from 9.5 to 12.5psi @19:42:01.71 SS.reconfigure :inhaling 2.20ml/s @19:42:01.76 SS.seek 25ml @19:42:09.59 Vacuum too high -- waiting up to 40 seconds @19:42:09.60 SS.reconfigure :exhale @19:42:09.65 SS.hold @19:42:14.84 Intake @17.6psia, vacuum from 10.0 to 13.0psi @19:42:14.86 SS.reconfigure :inhaling 1.76ml/s @19:42:14.91 SS.seek 25ml @19:42:18.81 Vacuum too high -- waiting up to 40 seconds @19:42:18.82 SS.reconfigure :exhale @19:42:18.87 SS.hold @19:42:21.15 Intake @17.8psia, vacuum from 10.0 to 13.0psi @19:42:21.17 SS.reconfigure :inhaling 1.41ml/s @19:42:21.22 SS.seek 25ml @19:42:24.97 Vacuum too high -- waiting up to 40 seconds @19:42:24.99 SS.reconfigure :exhale @19:42:25.04 SS.hold @19:42:27.21 Intake @18.5psia, vacuum from 10.0 to 13.0psi @19:42:27.22 SS.reconfigure :inhaling 1.13ml/s @19:42:27.27 SS.seek 25ml @19:42:30.85 Vacuum too high -- waiting up to 40 seconds @19:42:30.86 SS.reconfigure :exhale @19:42:30.91 SS.hold @19:42:32.28 SS.reconfigure :inhaling 0.91ml/s @19:42:32.33 SS.seek 25ml @19:42:34.30 Stroke completed -- waiting for pressure to equalize ... @19:42:34.31 SS.reconfigure :exhale @19:42:34.36 SS.hold @19:42:55.06 Intake.select :close @19:42:55.21 SSV.dial :exhaust @19:42:55.97 Exhaust.select :open @19:42:56.09 SS.seek 0ml @19:43:05.16 Exhaust.select :close @19:43:05.27 SSV.dial CBV @19:43:05.29
CBV.dial SSV @19:43:06.08 Allowing 130s for 25.0ml of remaining 975.0ml @19:43:06.09 Intake.select :open @19:43:08.26 Intake @15.6psia, vacuum from 10.0 to 13.0psi @19:43:08.28 SS.reconfigure :inhaling 0.91ml/s @19:43:08.33 SS.seek 25ml @19:43:37.69 Stroke completed -- waiting for pressure to equalize ... @19:43:37.70 SS.reconfigure :exhale @19:43:37.74 SS.hold @19:44:00.09 Intake.select :close @19:44:00.24 SSV.dial :exhaust @19:44:01.02 Exhaust.select :open @19:44:01.14 SS.seek 0ml @19:44:10.17 Exhaust.select :close @19:44:10.28 SSV.dial CBV @19:44:10.31
CBV.dial SSV @19:44:11.10 Allowing 130s for 25.0ml of remaining 950.0ml @19:44:11.11 Intake.select :open @19:44:13.27 Intake @15.7psia, vacuum from 10.0 to 13.0psi @19:44:13.28 SS.reconfigure :inhaling 0.91ml/s @19:44:13.33 SS.seek 25ml @19:44:42.94 Stroke completed -- waiting for pressure to equalize ... @19:44:42.95 SS.reconfigure :exhale @19:44:42.99 SS.hold @19:45:03.38 Intake.select :close @19:45:03.53 SSV.dial :exhaust @19:45:04.29 Exhaust.select :open @19:45:04.41 SS.seek 0ml @19:45:13.05 Exhaust.select :close @19:45:13.16 SSV.dial CBV @19:45:13.18
CBV.dial SSV @19:45:13.95 Allowing 130s for 25.0ml of remaining 925.0ml @19:45:13.97 Intake.select :open @19:45:16.13 Intake @15.7psia, vacuum from 10.0 to 13.0psi @19:45:16.15 SS.reconfigure :inhaling 0.91ml/s @19:45:16.20 SS.seek 25ml @19:45:46.42 Stroke completed -- waiting for pressure to equalize ... @19:45:46.43 SS.reconfigure :exhale @19:45:46.48 SS.hold @19:46:04.83 Intake.select :close @19:46:04.99 SSV.dial :exhaust @19:46:05.75 Exhaust.select :open @19:46:05.86 SS.seek 0ml @19:46:14.81 Exhaust.select :close @19:46:14.92 SSV.dial CBV @19:46:14.94
CBV.dial SSV @19:46:15.77 Allowing 130s for 25.0ml of remaining 900.0ml @19:46:15.78 Intake.select :open @19:46:17.94 Intake @15.8psia, vacuum from 10.0 to 13.0psi @19:46:17.95 SS.reconfigure :inhaling 0.91ml/s @19:46:18.00 SS.seek 25ml @19:46:47.42 Stroke completed -- waiting for pressure to equalize ... @19:46:47.43 SS.reconfigure :exhale @19:46:47.48 SS.hold @19:47:05.83 Intake.select :close @19:47:06.41 SSV.dial :exhaust @19:47:07.19 Exhaust.select :open @19:47:07.30 SS.seek 0ml @19:47:16.05 Exhaust.select :close @19:47:16.15 SSV.dial CBV @19:47:16.17
CBV.dial SSV @19:47:16.95 Allowing 130s for 25.0ml of remaining 875.0ml @19:47:16.96 Intake.select :open @19:47:19.13 Intake @15.8psia, vacuum from 10.0 to 13.0psi @19:47:19.15 SS.reconfigure :inhaling 0.91ml/s @19:47:19.20 SS.seek 25ml @19:47:49.04 Stroke completed -- waiting for pressure to equalize ... @19:47:49.05 SS.reconfigure :exhale @19:47:49.10 SS.hold @19:48:05.39 Intake.select :close @19:48:05.55 SSV.dial :exhaust @19:48:06.31 Exhaust.select :open @19:48:06.42 SS.seek 0ml @19:48:15.00 Exhaust.select :close @19:48:15.10 SSV.dial CBV @19:48:15.12
CBV.dial SSV @19:48:15.90 Allowing 130s for 25.0ml of remaining 850.0ml @19:48:15.92 Intake.select :open @19:48:18.08 Intake @15.7psia, vacuum from 10.0 to 13.0psi @19:48:18.09 SS.reconfigure :inhaling 0.91ml/s @19:48:18.14 SS.seek 25ml @19:48:48.60 Stroke completed -- waiting for pressure to equalize ... @19:48:48.61 SS.reconfigure :exhale @19:48:48.66 SS.hold @19:49:04.94 Intake.select :close @19:49:05.09 SSV.dial :exhaust @19:49:05.86 Exhaust.select :open @19:49:05.97 SS.seek 0ml @19:49:14.75 Exhaust.select :close @19:49:14.85 SSV.dial CBV @19:49:14.87
CBV.dial SSV @19:49:15.68 Allowing 130s for 25.0ml of remaining 825.0ml @19:49:15.70 Intake.select :open @19:49:17.86 Intake @15.7psia, vacuum from 10.0 to 13.0psi @19:49:17.87 SS.reconfigure :inhaling 0.91ml/s @19:49:17.92 SS.seek 25ml @19:49:47.18 Stroke completed -- waiting for pressure to equalize ... @19:49:47.19 SS.reconfigure :exhale @19:49:47.24 SS.hold @19:50:03.62 Intake.select :close @19:50:03.77 SSV.dial :exhaust @19:50:04.54 Exhaust.select :open @19:50:04.66 SS.seek 0ml @19:50:13.35 Exhaust.select :close @19:50:13.46 SSV.dial CBV @19:50:13.49
CBV.dial SSV @19:50:14.26 Allowing 130s for 25.0ml of remaining 800.0ml @19:50:14.27 Intake.select :open @19:50:16.44 Intake @15.8psia, vacuum from 10.0 to 13.0psi @19:50:16.45 SS.reconfigure :inhaling 0.91ml/s @19:50:16.50 SS.seek 25ml @19:50:46.41 Stroke completed -- waiting for pressure to equalize ... @19:50:46.42 SS.reconfigure :exhale @19:50:46.47 SS.hold @19:51:02.76 Intake.select :close @19:51:02.92 SSV.dial :exhaust @19:51:03.68 Exhaust.select :open @19:51:03.80 SS.seek 0ml @19:51:12.60 Exhaust.select :close @19:51:12.71 SSV.dial CBV @19:51:12.74
CBV.dial SSV @19:51:13.51 Allowing 130s for 25.0ml of remaining 775.0ml @19:51:13.52 Intake.select :open @19:51:15.69 Intake @15.6psia, vacuum from 10.0 to 13.0psi @19:51:15.70 SS.reconfigure :inhaling 0.91ml/s @19:51:15.75 SS.seek 25ml @19:51:45.23 Stroke completed -- waiting for pressure to equalize ... @19:51:45.24 SS.reconfigure :exhale @19:51:45.28 SS.hold @19:52:00.13 Intake.select :close @19:52:00.28 SSV.dial :exhaust @19:52:01.04 Exhaust.select :open @19:52:01.15 SS.seek 0ml @19:52:09.84 Exhaust.select :close @19:52:09.94 SSV.dial CBV @19:52:09.96
CBV.dial SSV @19:52:10.77 Allowing 130s for 25.0ml of remaining 750.0ml @19:52:10.79 Intake.select :open @19:52:12.95 Intake @15.7psia, vacuum from 10.0 to 13.0psi @19:52:12.97 SS.reconfigure :inhaling 0.91ml/s @19:52:13.02 SS.seek 25ml @19:52:42.99 Stroke completed -- waiting for pressure to equalize ... @19:52:43.00 SS.reconfigure :exhale @19:52:43.05 SS.hold @19:52:59.24 Intake.select :close @19:52:59.40 SSV.dial :exhaust @19:53:00.18 Exhaust.select :open @19:53:00.66 SS.seek 0ml @19:53:09.74 Exhaust.select :close @19:53:09.85 SSV.dial CBV @19:53:09.87
CBV.dial SSV @19:53:10.70 Allowing 130s for 25.0ml of remaining 725.0ml @19:53:10.71 Intake.select :open @19:53:12.87 Intake @15.7psia, vacuum from 10.0 to 13.0psi @19:53:12.89 SS.reconfigure :inhaling 0.91ml/s @19:53:12.94 SS.seek 25ml @19:53:41.85 Stroke completed -- waiting for pressure to equalize ... @19:53:41.86 SS.reconfigure :exhale @19:53:41.91 SS.hold @19:54:00.14 Intake.select :close @19:54:00.29 SSV.dial :exhaust @19:54:01.05 Exhaust.select :open @19:54:01.17 SS.seek 0ml @19:54:09.87 Exhaust.select :close @19:54:09.97 SSV.dial CBV @19:54:10.01
CBV.dial SSV @19:54:10.76 Allowing 130s for 25.0ml of remaining 700.0ml @19:54:10.77 Intake.select :open @19:54:12.93 Intake @15.6psia, vacuum from 10.0 to 13.0psi @19:54:12.94 SS.reconfigure :inhaling 0.91ml/s @19:54:12.99 SS.seek 25ml @19:54:43.25 Stroke completed -- waiting for pressure to equalize ... @19:54:43.26 SS.reconfigure :exhale @19:54:43.30 SS.hold @19:55:01.65 Intake.select :close @19:55:01.80 SSV.dial :exhaust @19:55:02.57 Exhaust.select :open @19:55:02.68 SS.seek 0ml @19:55:11.69 Exhaust.select :close @19:55:11.81 SSV.dial CBV @19:55:11.84
CBV.dial SSV @19:55:12.60 Allowing 130s for 25.0ml of remaining 675.0ml @19:55:12.61 Intake.select :open @19:55:14.78 Intake @15.7psia, vacuum from 10.0 to 13.0psi @19:55:14.79 SS.reconfigure :inhaling 0.91ml/s @19:55:14.84 SS.seek 25ml @19:55:44.36 Stroke completed -- waiting for pressure to equalize ... @19:55:44.37 SS.reconfigure :exhale @19:55:44.42 SS.hold @19:56:03.09 Intake.select :close @19:56:03.24 SSV.dial :exhaust @19:56:04.02 Exhaust.select :open @19:56:04.13 SS.seek 0ml @19:56:12.86 Exhaust.select :close @19:56:12.97 SSV.dial CBV @19:56:13.00
CBV.dial SSV @19:56:13.79 Allowing 130s for 25.0ml of remaining 650.0ml @19:56:13.80 Intake.select :open @19:56:15.96 Intake @15.6psia, vacuum from 10.0 to 13.0psi @19:56:15.97 SS.reconfigure :inhaling 0.91ml/s @19:56:16.02 SS.seek 25ml @19:56:45.99 Stroke completed -- waiting for pressure to equalize ... @19:56:46.00 SS.reconfigure :exhale @19:56:46.04 SS.hold @19:57:04.38 Intake.select :close @19:57:04.54 SSV.dial :exhaust @19:57:05.30 Exhaust.select :open @19:57:05.41 SS.seek 0ml @19:57:14.35 Exhaust.select :close @19:57:14.45 SSV.dial CBV @19:57:14.47
CBV.dial SSV @19:57:15.27 Allowing 130s for 25.0ml of remaining 625.0ml @19:57:15.28 Intake.select :open @19:57:17.44 Intake @15.8psia, vacuum from 10.0 to 13.0psi @19:57:17.46 SS.reconfigure :inhaling 0.91ml/s @19:57:17.51 SS.seek 25ml @19:57:47.05 Stroke completed -- waiting for pressure to equalize ... @19:57:47.06 SS.reconfigure :exhale @19:57:47.10 SS.hold @19:58:09.57 Intake.select :close @19:58:09.72 SSV.dial :exhaust @19:58:10.49 Exhaust.select :open @19:58:10.60 SS.seek 0ml @19:58:19.25 Exhaust.select :close @19:58:19.36 SSV.dial CBV @19:58:19.38
CBV.dial SSV @19:58:20.19 Allowing 130s for 25.0ml of remaining 600.0ml @19:58:20.20 Intake.select :open @19:58:22.37 Intake @15.7psia, vacuum from 10.0 to 13.0psi @19:58:22.38 SS.reconfigure :inhaling 0.91ml/s @19:58:22.43 SS.seek 25ml @19:58:52.44 Stroke completed -- waiting for pressure to equalize ... @19:58:52.45 SS.reconfigure :exhale @19:58:52.49 SS.hold @19:59:14.99 Intake.select :close @19:59:15.14 SSV.dial :exhaust @19:59:15.92 Exhaust.select :open @19:59:16.04 SS.seek 0ml @19:59:24.77 Exhaust.select :close @19:59:24.87 SSV.dial CBV @19:59:24.89
CBV.dial SSV @19:59:25.67 Allowing 130s for 25.0ml of remaining 575.0ml @19:59:25.69 Intake.select :open @19:59:27.85 Intake @15.9psia, vacuum from 10.0 to 13.0psi @19:59:27.86 SS.reconfigure :inhaling 0.91ml/s @19:59:27.91 SS.seek 25ml @19:59:50.91 Vacuum too high -- waiting up to 40 seconds @19:59:50.93 SS.reconfigure :exhale @19:59:50.98 SS.hold @19:59:59.63 Intake @15.2psia, vacuum from 9.7 to 12.7psi @19:59:59.65 SS.reconfigure :inhaling 0.73ml/s @19:59:59.70 SS.seek 25ml @20:00:08.50 Stroke completed -- waiting for pressure to equalize ... @20:00:08.51 SS.reconfigure :exhale @20:00:08.91 SS.hold @20:00:23.41 Intake.select :close @20:00:23.56 SSV.dial :exhaust @20:00:24.31 Exhaust.select :open @20:00:24.43 SS.seek 0ml @20:00:33.39 Exhaust.select :close @20:00:33.50 SSV.dial CBV @20:00:33.52
CBV.dial SSV @20:00:34.30 Allowing 130s for 25.0ml of remaining 550.0ml @20:00:34.31 Intake.select :open @20:00:36.47 Intake @15.9psia, vacuum from 10.0 to 13.0psi @20:00:36.48 SS.reconfigure :inhaling 0.73ml/s @20:00:36.53 SS.seek 25ml @20:01:12.81 Stroke completed -- waiting for pressure to equalize ... @20:01:12.82 SS.reconfigure :exhale @20:01:12.87 SS.hold @20:01:33.90 Intake.select :close @20:01:34.05 SSV.dial :exhaust @20:01:34.81 Exhaust.select :open @20:01:34.92 SS.seek 0ml @20:01:43.76 Exhaust.select :close @20:01:43.87 SSV.dial CBV @20:01:43.90
CBV.dial SSV @20:01:44.67 Allowing 130s for 25.0ml of remaining 525.0ml @20:01:44.68 Intake.select :open @20:01:46.85 Intake @15.7psia, vacuum from 10.0 to 13.0psi @20:01:46.86 SS.reconfigure :inhaling 0.73ml/s @20:01:46.91 SS.seek 25ml @20:02:22.87 Stroke completed -- waiting for pressure to equalize ... @20:02:22.88 SS.reconfigure :exhale @20:02:22.93 SS.hold @20:02:47.49 Intake.select :close @20:02:47.65 SSV.dial :exhaust @20:02:48.42 Exhaust.select :open @20:02:48.53 SS.seek 0ml @20:02:57.37 Exhaust.select :close @20:02:57.48 SSV.dial CBV @20:02:57.50
CBV.dial SSV @20:02:58.31 Allowing 130s for 25.0ml of remaining 500.0ml @20:02:58.32 Intake.select :open @20:03:00.49 Intake @15.7psia, vacuum from 10.0 to 13.0psi @20:03:00.50 SS.reconfigure :inhaling 0.73ml/s @20:03:00.55 SS.seek 25ml @20:03:36.48 Stroke completed -- waiting for pressure to equalize ... @20:03:36.49 SS.reconfigure :exhale @20:03:36.53 SS.hold @20:04:03.37 Intake.select :close @20:04:03.53 SSV.dial :exhaust @20:04:04.30 Exhaust.select :open @20:04:04.41 SS.seek 0ml @20:04:13.35 Exhaust.select :close @20:04:13.46 SSV.dial CBV @20:04:13.49
CBV.dial SSV @20:04:14.26 Allowing 130s for 25.0ml of remaining 475.0ml @20:04:14.27 Intake.select :open @20:04:16.44 Intake @15.8psia, vacuum from 10.0 to 13.0psi @20:04:16.45 SS.reconfigure :inhaling 0.73ml/s @20:04:16.50 SS.seek 25ml @20:04:49.19 Vacuum too high -- waiting up to 40 seconds @20:04:49.20 SS.reconfigure :exhale @20:04:49.25 SS.hold @20:05:02.06 Intake @16.0psia, vacuum from 10.0 to 13.0psi @20:05:02.08 SS.reconfigure :inhaling 0.59ml/s @20:05:02.13 SS.seek 25ml @20:05:06.44 Stroke completed -- waiting for pressure to equalize ... @20:05:06.46 SS.reconfigure :exhale @20:05:06.50 SS.hold @20:05:22.80 Intake.select :close @20:05:22.95 SSV.dial :exhaust @20:05:23.71 Exhaust.select :open @20:05:23.83 SS.seek 0ml @20:05:32.71 Exhaust.select :close @20:05:32.82 SSV.dial CBV @20:05:32.84
CBV.dial SSV @20:05:33.65 Allowing 130s for 25.0ml of remaining 450.0ml @20:05:33.66 Intake.select :open @20:05:35.83 Intake @15.7psia, vacuum from 10.0 to 13.0psi @20:05:35.84 SS.reconfigure :inhaling 0.59ml/s @20:05:35.90 SS.seek 25ml @20:06:20.26 Stroke completed -- waiting for pressure to equalize ... @20:06:20.27 SS.reconfigure :exhale @20:06:20.31 SS.hold @20:06:44.89 Intake.select :close @20:06:45.05 SSV.dial :exhaust @20:06:45.82 Exhaust.select :open @20:06:45.94 SS.seek 0ml @20:06:54.55 Exhaust.select :close @20:06:54.66 SSV.dial CBV @20:06:54.70
CBV.dial SSV @20:06:55.46 Allowing 130s for 25.0ml of remaining 425.0ml @20:06:55.47 Intake.select :open @20:06:57.64 Intake @15.9psia, vacuum from 10.0 to 13.0psi @20:06:57.66 SS.reconfigure :inhaling 0.59ml/s @20:06:57.71 SS.seek 25ml @20:07:35.34 Vacuum too high -- waiting up to 40 seconds @20:07:35.35 SS.reconfigure :exhale @20:07:35.41 SS.hold @20:07:48.19 Intake @16.8psia, vacuum from 10.0 to 13.0psi @20:07:48.21 SS.reconfigure :inhaling 0.47ml/s @20:07:48.25 SS.seek 25ml @20:07:57.55 Stroke completed -- waiting for pressure to equalize ... @20:07:57.56 SS.reconfigure :exhale @20:07:57.61 SS.hold @20:08:12.30 Intake.select :close @20:08:12.46 SSV.dial :exhaust @20:08:13.24 Exhaust.select :open @20:08:13.36 SS.seek 0ml @20:08:22.13 Exhaust.select :close @20:08:22.24 SSV.dial CBV @20:08:22.26
CBV.dial SSV @20:08:23.07 Allowing 130s for 25.0ml of remaining 400.0ml @20:08:23.08 Intake.select :open @20:08:25.24 Intake @15.9psia, vacuum from 10.0 to 13.0psi @20:08:25.26 SS.reconfigure :inhaling 0.47ml/s @20:08:25.31 SS.seek 25ml @20:09:20.86 Stroke completed -- waiting for pressure to equalize ... @20:09:20.87 SS.reconfigure :exhale @20:09:20.92 SS.hold @20:09:45.40 Intake.select :close @20:09:45.55 SSV.dial :exhaust @20:09:46.30 Exhaust.select :open @20:09:46.42 SS.seek 0ml @20:09:55.11 Exhaust.select :close @20:09:55.22 SSV.dial CBV @20:09:55.24
CBV.dial SSV @20:09:56.04 Allowing 130s for 25.0ml of remaining 375.0ml @20:09:56.05 Intake.select :open @20:09:58.21 Intake @15.8psia, vacuum from 10.0 to 13.0psi @20:09:58.23 SS.reconfigure :inhaling 0.47ml/s @20:09:58.28 SS.seek 25ml @20:10:53.95 Stroke completed -- waiting for pressure to equalize ... @20:10:53.96 SS.reconfigure :exhale @20:10:54.01 SS.hold @20:11:20.54 Intake.select :close @20:11:20.69 SSV.dial :exhaust @20:11:21.46 Exhaust.select :open @20:11:21.58 SS.seek 0ml @20:11:30.20 Exhaust.select :close @20:11:30.31 SSV.dial CBV @20:11:30.34
CBV.dial SSV @20:11:31.13 Allowing 130s for 25.0ml of remaining 350.0ml @20:11:31.14 Intake.select :open @20:11:33.31 Intake @15.8psia, vacuum from 10.0 to 13.0psi @20:11:33.32 SS.reconfigure :inhaling 0.47ml/s @20:11:33.37 SS.seek 25ml @20:12:29.03 Stroke completed -- waiting for pressure to equalize ... @20:12:29.04 SS.reconfigure :exhale @20:12:29.09 SS.hold @20:12:58.07 Intake.select :close @20:12:58.25 SSV.dial :exhaust @20:12:59.03 Exhaust.select :open @20:12:59.15 SS.seek 0ml @20:13:08.16 Exhaust.select :close @20:13:08.26 SSV.dial CBV @20:13:08.29
CBV.dial SSV @20:13:09.06 Allowing 130s for 25.0ml of remaining 325.0ml @20:13:09.07 Intake.select :open @20:13:11.25 Intake @15.8psia, vacuum from 10.0 to 13.0psi @20:13:11.26 SS.reconfigure :inhaling 0.47ml/s @20:13:11.31 SS.seek 25ml @20:14:06.62 Stroke completed -- waiting for pressure to equalize ... @20:14:06.63 SS.reconfigure :exhale @20:14:06.68 SS.hold @20:14:39.35 Intake.select :close @20:14:39.50 SSV.dial :exhaust @20:14:40.28 Exhaust.select :open @20:14:40.39 SS.seek 0ml @20:14:49.39 Exhaust.select :close @20:14:49.50 SSV.dial CBV @20:14:49.52
CBV.dial SSV @20:14:50.30 Allowing 130s for 25.0ml of remaining 300.0ml @20:14:50.31 Intake.select :open @20:14:52.47 Intake @15.9psia, vacuum from 10.0 to 13.0psi @20:14:52.48 SS.reconfigure :inhaling 0.47ml/s @20:14:52.53 SS.seek 25ml @20:15:47.87 Stroke completed -- waiting for pressure to equalize ... @20:15:47.88 SS.reconfigure :exhale @20:15:47.93 SS.hold @20:16:22.93 Intake.select :close @20:16:23.09 SSV.dial :exhaust @20:16:23.86 Exhaust.select :open @20:16:23.97 SS.seek 0ml @20:16:33.03 Exhaust.select :close @20:16:33.13 SSV.dial CBV @20:16:33.15
CBV.dial SSV @20:16:33.96 Allowing 130s for 25.0ml of remaining 275.0ml @20:16:33.98 Intake.select :open @20:16:36.49 Intake @15.9psia, vacuum from 10.0 to 13.0psi @20:16:36.58 SS.reconfigure :inhaling 0.47ml/s @20:16:36.63 SS.seek 25ml @20:17:22.39 Vacuum too high -- waiting up to 40 seconds @20:17:22.41 SS.reconfigure :exhale @20:17:22.46 SS.hold @20:17:40.93 Intake @17.0psia, vacuum from 10.0 to 13.0psi @20:17:40.95 SS.reconfigure :inhaling 0.38ml/s @20:17:41.00 SS.seek 25ml @20:17:52.54 Stroke completed -- waiting for pressure to equalize ... @20:17:52.55 SS.reconfigure :exhale @20:17:52.60 SS.hold @20:18:17.40 Intake.select :close @20:18:17.56 SSV.dial :exhaust @20:18:18.33 Exhaust.select :open @20:18:18.44 SS.seek 0ml @20:18:27.24 Exhaust.select :close @20:18:27.36 SSV.dial CBV @20:18:27.40
CBV.dial SSV @20:18:28.13 Allowing 130s for 25.0ml of remaining 250.0ml @20:18:28.15 Intake.select :open @20:18:30.31 Intake @15.9psia, vacuum from 10.0 to 13.0psi @20:18:30.33 SS.reconfigure :inhaling 0.38ml/s @20:18:30.38 SS.seek 25ml @20:19:39.65 Stroke completed -- waiting for pressure to equalize ... @20:19:39.66 SS.reconfigure :exhale @20:19:39.70 SS.hold @20:20:16.49 Intake.select :close @20:20:16.64 SSV.dial :exhaust @20:20:17.40 Exhaust.select :open @20:20:17.51 SS.seek 0ml @20:20:26.57 Exhaust.select :close @20:20:26.68 SSV.dial CBV @20:20:26.70
CBV.dial SSV @20:20:27.49 Allowing 130s for 25.0ml of remaining 225.0ml @20:20:27.50 Intake.select :open @20:20:29.67 Intake @15.9psia, vacuum from 10.0 to 13.0psi @20:20:29.68 SS.reconfigure :inhaling 0.38ml/s @20:20:29.73 SS.seek 25ml @20:21:37.04 Vacuum too high -- waiting up to 40 seconds @20:21:37.06 SS.reconfigure :exhale @20:21:37.11 SS.hold @20:21:55.93 Intake @16.5psia, vacuum from 10.0 to 13.0psi @20:21:55.94 SS.reconfigure :inhaling 0.31ml/s @20:21:55.99 SS.seek 25ml @20:21:57.80 Stroke completed -- waiting for pressure to equalize ... @20:21:57.81 SS.reconfigure :exhale @20:21:57.86 SS.hold @20:22:18.61 Intake.select :close @20:22:18.76 SSV.dial :exhaust @20:22:19.52 Exhaust.select :open @20:22:19.63 SS.seek 0ml @20:22:28.41 Exhaust.select :close @20:22:28.53 SSV.dial CBV @20:22:28.56
CBV.dial SSV @20:22:29.32 Allowing 130s for 25.0ml of remaining 200.0ml @20:22:29.33 Intake.select :open @20:22:31.49 Intake @15.9psia, vacuum from 10.0 to 13.0psi @20:22:31.51 SS.reconfigure :inhaling 0.31ml/s @20:22:31.56 SS.seek 25ml @20:24:01.06 Stroke completed -- waiting for pressure to equalize ... @20:24:01.07 SS.reconfigure :exhale @20:24:01.12 SS.hold @20:24:35.82 Intake.select :close @20:24:35.97 SSV.dial :exhaust @20:24:36.74 Exhaust.select :open @20:24:36.85 SS.seek 0ml @20:24:45.66 Exhaust.select :close @20:24:45.77 SSV.dial CBV @20:24:45.79
CBV.dial SSV @20:24:46.58 Allowing 130s for 25.0ml of remaining 175.0ml @20:24:46.59 Intake.select :open @20:24:48.76 Intake @16.0psia, vacuum from 10.0 to 13.0psi @20:24:48.77 SS.reconfigure :inhaling 0.31ml/s @20:24:48.82 SS.seek 25ml @20:26:19.55 Stroke completed -- waiting for pressure to equalize ... @20:26:19.56 SS.reconfigure :exhale @20:26:19.60 SS.hold @20:26:56.60 SS.reconfigure :default @20:26:56.65 SS.hold @20:26:56.71 Intake.select :close @20:26:56.86 ShallowSampler::Clogged in MAIN -- Filtering too Slowly after 850.0ml @20:26:56.88 SSV.dial :exhaust @20:26:57.64 Exhaust.select :open @20:26:57.75 SS.reconfigure :exhale @20:26:57.80 SS.seek 0ml @20:27:06.83 Exhaust.select :close @20:27:06.93 Sampled 850.0ml @20:27:06.95 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @20:27:06.96 Intake.select :close @20:27:07.02 CSV.dial :waste @20:27:07.16 SSV.dial CBV,avoiding: :exhaust @20:27:07.17
CBV.dial SSV @20:27:07.30 CS.seek 0ml @20:27:07.37 CSV.dial :air @20:27:08.10 CS.seek 10ml @20:27:09.11
CTV.dial :air @20:27:10.31 delay 15 seconds @20:27:25.33 SSV.dial :spit,avoiding: :exhaust @20:27:26.68 CTV.dial CSV,avoiding: :intake @20:27:26.71
SS.seek 0ml @20:27:26.84 SSV.dial CBV,avoiding: :exhaust @20:27:32.77 SSV.dial between CBV and plug @20:27:33.27 CBV.dial between SSV and waste2 @20:27:33.65 CSV.dial between air and CTV @20:27:33.97 CS.seek 8.328ml @20:27:38.61 CTV.dial CSV,avoiding: :air @20:27:38.63
CSV.dial CTV @20:27:38.97 CS.reconfigure :pressurize @20:27:39.02 CS.seek 0ml @20:27:52.93 BubblePoint: Intake @24.2psia, Outlet @0.9psia @20:27:52.94 CBV.dial SSV @20:27:53.27 SS.reconfigure :default @20:27:53.32 SS.seek 10ml @20:27:58.80 delay 15 seconds @20:28:13.93 Evacuated: Intake @23.8psia, Outlet @23.4psia @20:28:13.94 SSV.dial :spit,avoiding: :exhaust @20:28:14.97 SS.reconfigure :exhale @20:28:15.02 SS.seek 0ml @20:28:19.98 SS.reconfigure :fast @20:28:20.02 SS.seek 5ml @20:28:22.83 SSV.dial CBV,avoiding: :exhaust @20:28:22.85
CBV.dial SSV @20:28:24.25 CTV.dial :air,avoiding: :intake @20:28:25.59 CSV.dial CTV @20:28:25.65
SS.seek 10ml @20:28:26.80 CSV.dial :air @20:28:27.25 CS.reconfigure :fast @20:28:27.30 CS.seek 0ml @20:28:28.16
SSV.dial :spit,avoiding: :exhaust @20:28:29.51 SS.reconfigure :exhale @20:28:29.55 SS.seek 0ml @20:28:34.60 SS.reconfigure :fast @20:28:34.65 SS.seek 5ml @20:28:35.29 CS.stop @20:28:37.60 SSV.dial CBV,avoiding: :exhaust @20:28:37.62
CBV.dial SSV @20:28:39.98 SSV.dial :spit,avoiding: :exhaust @20:28:41.32 SS.reconfigure :default @20:28:41.36 SS.seek 0ml @20:28:44.46 SS.stop @20:28:44.93 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @20:28:44.94 Intake.select :close @20:28:45.02 CSV.dial :waste @20:28:45.15 SSV.dial CBV,avoiding: :exhaust @20:28:45.16
CBV.dial SSV @20:28:45.80 CS.seek 0ml @20:28:45.86 CSV.dial :air @20:28:46.52
CTV.dial :air @20:28:46.53 delay 15 seconds @20:28:46.60 CS.seek 10ml @20:29:01.55
SSV.dial :spit,avoiding: :exhaust @20:29:02.90 CTV.dial CSV,avoiding: :intake @20:29:02.93
SS.reconfigure :exhale @20:29:03.02 SS.seek 0ml @20:29:03.45 SSV.dial CBV,avoiding: :exhaust @20:29:11.20 SSV.dial between CBV and plug @20:29:11.69 CBV.dial between SSV and waste2 @20:29:12.09 CSV.dial between air and CTV @20:29:12.40 CS.seek 7.466ml @20:29:18.93 CTV.dial CSV,avoiding: :air @20:29:18.95
CSV.dial CTV @20:29:19.30 CS.reconfigure :pressurize @20:29:19.35 CS.seek 0ml @20:29:34.99 BubblePoint: Intake @24.2psia, Outlet @3.2psia @20:29:35.00 CBV.dial SSV @20:29:35.34 SS.reconfigure :default @20:29:35.39 SS.seek 10ml @20:29:41.03 delay 15 seconds @20:29:56.16 Evacuated: Intake @24.0psia, Outlet @23.6psia @20:29:56.17 SSV.dial :spit,avoiding: :exhaust @20:29:57.22 SS.reconfigure :exhale @20:29:57.27 SS.seek 0ml @20:30:02.31 SS.reconfigure :fast @20:30:02.36 SS.seek 5ml @20:30:05.34 SSV.dial CBV,avoiding: :exhaust @20:30:05.37
CBV.dial SSV @20:30:06.73 CTV.dial :air,avoiding: :intake @20:30:08.08 CSV.dial CTV @20:30:08.13
SS.seek 10ml @20:30:09.30 CSV.dial :air @20:30:09.73 CS.reconfigure :fast @20:30:09.78 CS.seek 0ml @20:30:10.84
SSV.dial :spit,avoiding: :exhaust @20:30:12.17 SS.reconfigure :exhale @20:30:12.22 SS.seek 0ml @20:30:14.45 CS.stop @20:30:17.41
SS.reconfigure :fast @20:30:17.46 SS.seek 5ml @20:30:20.47 SSV.dial CBV,avoiding: :exhaust @20:30:20.49
CBV.dial SSV @20:30:22.86 SSV.dial :spit,avoiding: :exhaust @20:30:24.20 SS.reconfigure :default @20:30:24.25 SS.seek 0ml @20:30:27.28 SS.stop @20:30:27.32 Lysing: Adding 2.0ml AqMeOH @20:30:27.33 CBV.dial between CSV and waste1,avoiding: :DBV @20:30:28.84 CTV.dial CSV @20:30:30.15 CSV.dial CSR @20:30:30.94 Pulling 1ml of CSR.AqMeOH @20:30:30.95 CSR.select :AqMeOH @20:30:31.06 CS.reconfigure :default @20:30:31.11 CS.seek 1ml @20:30:34.79 delay 5 seconds @20:30:39.81 CSR.select :air @20:30:39.98 CS.seek 6ml @20:30:53.82 delay 2 seconds @20:30:55.84 CSV.dial :waste2 @20:30:56.41 CS.reconfigure :fast @20:30:56.45 CS.seek 0ml @20:31:10.92 CSV.dial CSR @20:31:11.35 Pulling 2ml of CSR.AqMeOH @20:31:11.36 CSR.select :AqMeOH @20:31:11.47 CS.reconfigure :default @20:31:11.52 CS.seek 2ml @20:31:17.54 delay 5 seconds @20:31:22.56 CSR.select :air @20:31:22.73 CS.seek 5ml @20:31:31.42 delay 2 seconds @20:31:33.43 CSV.dial CTV @20:31:33.88 CS.seek 1ml @20:31:45.06 Before lysing Collection Puck Heater at 13.8C/31.0psia @20:31:45.08 Heating Puck.from 3, :sh1 in Collection Clamp to 70C for 10:00 @20:31:45.09 CH.seek 70C,8:00 @20:33:59.17 CH.hold 10:00,70C @20:33:59.37 Begun lysing Collection Puck Heater at 68.1C/38.6psia @20:43:59.82 Ended lysing Collection Puck Heater at 70C/37.7psia @20:43:59.84 CS.seek 0ml @20:44:03.25 CTV.dial between spare and air,avoiding: :intake @20:44:04.31 CSV.dial :air @20:44:04.77 CS.seek 5ml @20:44:18.53 CSV.dial CTV @20:44:18.98 delay 2 seconds @20:44:21.00 CSV.dial :air @20:44:21.45 delay 2 seconds @20:44:23.47 CSV.dial CTV @20:44:23.92 delay 2 seconds @20:44:25.94 CSV.dial :air @20:44:26.39 delay 2 seconds @20:44:28.41 CS.seek 0ml @20:44:42.05 Puck @84.3psia after final lysis pressurization @20:44:42.06 Recovering Lysate into Collection syringe @20:44:42.07 CSV.dial CBV @20:44:43.07 CBV.dial CSV @20:44:43.47 CS.seek 5ml @20:44:57.28 delay 5 seconds @20:45:02.30 CTV.dial :air @20:45:02.82 CS.seek 7ml @20:45:08.68 delay 5 seconds @20:45:13.70 CSV.dial :air2 @20:45:14.14 CS.seek 10ml @20:45:22.59 CSV.dial between air and CTV,avoiding: CBV @20:45:23.75 Lysing Complete @20:45:23.82 Storing sh1 puck in Collection Clamp to tube 2 @20:45:24.00 SC.reconfigure :tight @20:45:24.04 SC.seek 2 @20:45:27.54 SC.configure :limp @20:45:27.76 Elbow.reconfigure :tight @20:45:27.80 Elbow.seek 2 @20:45:29.58 Forearm.reconfigure :tight @20:45:29.63 Forearm.seek 2 + 450 counts @20:45:30.63 SE.reconfigure :pick @20:45:30.69 SE.seek :beamBreak @20:45:31.76 SE.configure :default @20:45:31.92 Elbow.reconfigure :limp @20:45:31.97 Forearm.reconfigure :loose @20:45:32.01 Forearm.seek 2 @20:45:32.52 Elbow.hold @20:45:32.58 Elbow.reconfigure :tight @20:45:32.69 SE.seek 30.08 Pucks below Top Plate @20:45:33.21 Forearm.force -20 @20:45:33.26 Forearm.seek 2 + 291 counts @20:45:33.65 Forearm.reconfigure :tight @20:45:33.70 Forearm.seek 2 + 450 counts @20:45:33.94 SE.seek 31.08 Pucks below Top Plate @20:45:34.59 Forearm.reconfigure :loose @20:45:34.64 Forearm.seek :retract @20:45:35.57 Elbow.seek CC @20:45:37.25 Elbow.reconfigure :loose @20:45:37.35 Forearm.seek CC @20:45:38.34 Hand.close @20:45:38.94 CC.seek :open @20:45:43.67 Forearm.seek :retract @20:45:44.69 Elbow.reconfigure :tight @20:45:44.73 Elbow.seek 2 @20:45:46.50 Elbow.reconfigure :limp @20:45:46.55 Forearm.seek 2 @20:45:47.51 Elbow.hold @20:45:47.58 Elbow.reconfigure :tight @20:45:47.62 Hand.open @20:45:48.31 Forearm.force -20 @20:45:48.35 Forearm.seek 2 + 294 counts @20:45:48.65 Forearm.reconfigure :tight @20:45:48.71 Forearm.seek 2 + 450 counts @20:45:48.97 SE.seek :down @20:45:50.05 SE.stop @20:45:50.18
Forearm.reconfigure :loose @20:45:50.23 Forearm.seek :retract @20:45:51.15 Tranferring DA @20:45:51.17 Beginning samplePassC @20:45:51.18 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @20:45:51.37 Elbow.seek FlushPuck::Garage @20:45:53.13 Elbow.reconfigure :loose @20:45:53.23 Forearm.seek :garage @20:45:54.26 Hand.close @20:45:55.05 Forearm.seek :retract @20:45:56.03 Elbow.reconfigure :tight @20:45:56.08 Elbow.seek CC @20:45:59.02 Elbow.reconfigure :loose @20:45:59.12 Forearm.reconfigure :tight @20:45:59.17 Forearm.seek CC @20:46:00.46 CC.seek :closed @20:46:04.97 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.39 @20:46:05.04 Hand.open @20:46:05.74 Forearm.reconfigure :loose @20:46:05.79 Forearm.seek :retract @20:46:06.87 PSV.dial PSR @20:46:08.29 Pulling 1.5ml of PSR.flush @20:46:08.30 PSR.select :flush @20:46:08.42 PS.seek 1.5ml @20:46:24.54 delay 5 seconds @20:46:29.56 PSV.dial :waste1 @20:46:30.04 PS.seek 0ml @20:46:46.22 PSV.dial PSR @20:46:46.70 PSR.select :air @20:46:46.82 PS.seek 2.5ml @20:47:13.19 PRV.dial CBV,avoiding: :ab2 @20:47:13.21
CBV.dial PRV @20:47:14.97 PSV.dial :mixloop @20:47:16.02 CTV.dial :air,avoiding: :intake @20:47:16.20 PTV.dial :PRVmixing @20:47:17.55 PS.seek 0ml @20:47:44.09 PSV.dial :air @20:47:44.57 PS.seek 2.5ml @20:48:10.96 PSV.dial :mixloop @20:48:11.44 PS.seek 0ml @20:48:37.81 PSV.dial :air @20:48:38.41 PS.seek 2.5ml @20:49:04.71 PSV.dial :mixloop @20:49:05.18 PS.seek 0ml @20:49:31.56 Garaging FlushPuck in Collection Clamp @20:49:31.63 Elbow.reconfigure :tight @20:49:31.68 Elbow.seek CC @20:49:32.19 Elbow.reconfigure :loose @20:49:32.29 Forearm.seek CC @20:49:33.26 Hand.close @20:49:33.93 CC.seek :open @20:49:38.54 Forearm.seek :retract @20:49:39.57 Elbow.reconfigure :tight @20:49:39.62 Elbow.seek :garage @20:49:42.58 Elbow.reconfigure :loose @20:49:42.68 Forearm.reconfigure :tight @20:49:42.73 Forearm.seek :garage @20:49:43.83 Hand.open @20:49:44.49 Forearm.reconfigure :loose @20:49:44.91 Forearm.seek :retract @20:49:45.96 PSV.dial :mixloop @20:49:46.04 PTV.dial :PRVmixing @20:49:46.23 PRV.dial CBV @20:49:46.25
CBV.dial PRV @20:49:46.28 CTV.dial :air,avoiding: :intake @20:49:46.29 Loading lyfil puck from tube 3 into Collection Clamp @20:49:46.53 SC.reconfigure :tight @20:49:46.58 SC.seek 3 @20:49:50.11 SC.configure :limp @20:49:50.33 Elbow.reconfigure :tight @20:49:50.38 Elbow.seek 3 @20:49:52.33 Forearm.reconfigure :tight @20:49:52.38 Forearm.seek 3 + 450 counts @20:49:53.44 SE.reconfigure :pick @20:49:53.50 SE.seek :beamBreak @20:49:56.55 SE.configure :default @20:49:56.71 Elbow.seek 3 @20:49:56.89 Forearm.seek 3 + 300 counts @20:49:57.07 Forearm.reconfigure :loose @20:49:57.12 Hand.close @20:49:57.70 SE.seek :down @20:49:57.90
Forearm.seek :retract @20:49:58.90 Elbow.seek CC @20:50:00.53 SE.stop @20:50:00.60
Elbow.reconfigure :loose @20:50:00.70 Forearm.reconfigure :tight @20:50:00.74 Forearm.seek CC @20:50:02.38 CC.seek :closed @20:50:06.91 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.17 @20:50:06.92 Hand.open @20:50:07.62 Forearm.reconfigure :loose @20:50:07.66 Forearm.seek :retract @20:50:08.65 CTV.dial CSV,avoiding: :intake @20:50:09.99 CSV.dial CTV @20:50:10.31 PS.seek 2.5ml @20:50:36.77 CS.seek 4.499ml @20:50:51.68 delay 8 seconds @20:50:59.70 PSV.dial :air @20:51:00.17 delay 2 seconds @20:51:02.18 PSV.dial :waste2 @20:51:02.94 PS.seek 0ml @20:51:29.31 CBV.dial between PRV and waste1 @20:51:29.63 CSV.dial :air @20:51:30.09 CS.seek 10ml @20:51:44.96 CSV.dial CTV @20:51:45.42 CS.seek 0ml @20:52:12.05 CBV.dial :waste1 @20:52:12.37 delay 5 seconds @20:52:17.39 CTV.dial :air,avoiding: :intake @20:52:18.80 Storing lyfil puck in Collection Clamp to tube 2 @20:52:18.98 SC.reconfigure :tight @20:52:19.02 SC.seek 2 @20:52:22.60 SC.configure :limp @20:52:22.81 Elbow.reconfigure :tight @20:52:22.86 Elbow.seek 2 @20:52:25.07 Forearm.reconfigure :tight @20:52:25.18 Forearm.seek 2 + 450 counts @20:52:26.21 SE.reconfigure :pick @20:52:26.27 SE.seek :beamBreak @20:52:27.26 SE.configure :default @20:52:27.42 Elbow.reconfigure :limp @20:52:27.47 Forearm.reconfigure :loose @20:52:27.52 Forearm.seek 2 @20:52:27.81 Elbow.hold @20:52:27.87 Elbow.reconfigure :tight @20:52:27.97 SE.seek 31.08 Pucks below Top Plate @20:52:28.46 Forearm.force -20 @20:52:28.50 Forearm.seek 2 + 317 counts @20:52:28.80 Forearm.reconfigure :tight @20:52:28.84 Forearm.seek 2 + 450 counts @20:52:29.06 SE.seek 32.08 Pucks below Top Plate @20:52:29.66 Forearm.reconfigure :loose @20:52:29.70 Forearm.seek :retract @20:52:30.68 Elbow.seek CC @20:52:32.44 Elbow.reconfigure :loose @20:52:32.54 Forearm.seek CC @20:52:33.47 Hand.close @20:52:34.06 CC.seek :open @20:52:38.79 Forearm.seek :retract @20:52:39.73 Elbow.reconfigure :tight @20:52:39.77 Elbow.seek 2 @20:52:41.56 Elbow.reconfigure :limp @20:52:41.98 Forearm.seek 2 @20:52:43.08 Elbow.hold @20:52:43.15 Elbow.reconfigure :tight @20:52:43.20 Hand.open @20:52:43.94 Forearm.force -20 @20:52:43.98 Forearm.seek 2 + 318 counts @20:52:44.28 Forearm.reconfigure :tight @20:52:44.33 Forearm.seek 2 + 450 counts @20:52:44.60 SE.seek :down @20:52:45.12 SE.stop @20:52:45.73
Forearm.reconfigure :loose @20:52:45.78 Forearm.seek :retract @20:52:46.71 PSV.dial :air @20:52:47.59 PS.seek 2.5ml @20:53:13.90 delay 1 second @20:53:14.91 PSV.dial :mixloop @20:53:15.40 delay 1 second @20:53:16.42 PSV.dial :air @20:53:16.90 delay 1 second @20:53:17.92 PSV.dial :mixloop @20:53:18.40 delay 1 second @20:53:19.42 PSV.dial :air @20:53:19.90 delay 1 second @20:53:20.92 PS.seek 0ml @20:53:47.46 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @20:53:47.65 Elbow.seek FlushPuck::Garage @20:53:49.48 Elbow.reconfigure :loose @20:53:49.58 Forearm.seek :garage @20:53:50.61 Hand.close @20:53:51.29 Forearm.seek :retract @20:53:52.27 Elbow.reconfigure :tight @20:53:52.32 Elbow.seek PC @20:53:53.59 Elbow.reconfigure :loose @20:53:54.11 Forearm.reconfigure :tight @20:53:54.17 Forearm.seek PC @20:53:55.39 PC.seek :closed @20:54:00.03 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.56 @20:54:00.10 Hand.open @20:54:00.79 Forearm.reconfigure :loose @20:54:00.84 Forearm.seek :retract @20:54:01.85 PRV.dial :air @20:54:02.32 PSV.dial :mixloop @20:54:02.86 PS.seek 0.3ml @20:54:06.71 PSV.dial PSR @20:54:07.76 Pulling 0.4ml of PSR.air @20:54:07.77 PSR.select :air @20:54:07.79 PS.seek 0.7ml @20:54:12.71 delay 3 seconds @20:54:15.73 PSR.select :air @20:54:15.75 PS.seek 2.5ml @20:54:35.02 delay 3 seconds @20:54:38.04 PSV.dial :mixloop @20:54:39.10 PTV.dial :mixing @20:54:39.87 PBV.dial :waste2 @20:54:40.83 PS.seek 0ml @20:55:07.24 Garaging FlushPuck in Processing Clamp @20:55:07.31 Elbow.reconfigure :tight @20:55:07.36 Elbow.seek PC @20:55:07.48 Elbow.reconfigure :loose @20:55:07.58 Forearm.seek PC @20:55:08.53 Hand.close @20:55:09.18 PC.seek :open @20:55:13.88 Forearm.seek :retract @20:55:15.31 Elbow.reconfigure :tight @20:55:15.37 Elbow.seek :garage @20:55:16.75 Elbow.reconfigure :loose @20:55:16.85 Forearm.reconfigure :tight @20:55:16.90 Forearm.seek :garage @20:55:17.97 Hand.open @20:55:18.61 Forearm.reconfigure :loose @20:55:18.66 Forearm.seek :retract @20:55:19.69 Loading da puck from tube 4 into Processing Clamp @20:55:19.93 SC.reconfigure :tight @20:55:19.98 SC.seek 4 @20:55:26.67 SC.configure :limp @20:55:26.88 Elbow.reconfigure :tight @20:55:26.93 Elbow.seek 4 @20:55:28.93 Forearm.reconfigure :tight @20:55:28.97 Forearm.seek 4 + 450 counts @20:55:30.05 SE.reconfigure :pick @20:55:30.11 SE.seek :beamBreak @20:55:31.03 SE.configure :default @20:55:31.19 Elbow.seek 4 @20:55:31.37 Forearm.seek 4 + 300 counts @20:55:31.63 Forearm.reconfigure :loose @20:55:31.69 Hand.close @20:55:32.31 SE.seek :down @20:55:32.52
Forearm.seek :retract @20:55:32.90 SE.stop @20:55:33.47
Elbow.seek PC @20:55:36.03 Elbow.reconfigure :loose @20:55:36.12 Forearm.reconfigure :tight @20:55:36.17 Forearm.seek PC @20:55:37.82 PC.seek :closed @20:55:42.49 Processing Clamp onSpring/offSpring for 16/26 samples, on/off torque 1.59 @20:55:42.50 Hand.open @20:55:43.11 Forearm.reconfigure :loose @20:55:43.16 Forearm.seek :retract @20:55:44.14 Heating Puck.from 4, :da in Processing Clamp to 28C for 2:00:00 @20:55:44.15 PH.seek 28C,5:00 @20:56:27.94 PH.hold 2:00:00,28C @20:56:27.99 delay 1:30 @20:57:58.04 PSR.select :air @20:57:58.06 PSV.dial PSR @20:57:59.11 Pulling 0.5ml of PSR.wash @20:57:59.12 PSR.select :wash @20:57:59.24 PS.seek 0.5ml @20:58:05.11 delay 5 seconds @20:58:10.13 PSR.select :air @20:58:10.25 PS.seek 2ml @20:58:26.39 PTV.dial :puck @20:58:27.14 PSV.dial PTV @20:58:27.91 PBV.dial :waste1 @20:58:29.31 PS.seek 1.5ml @20:58:35.24 delay 3 seconds @20:58:38.25 PBV.dial between PSV and waste1 @20:58:38.62 PS.seek 0ml @20:58:54.74 delay 5:00 @21:03:54.76 recoverPSRreagent08 @21:03:54.77 PTV.dial :puck @21:03:54.84 PBV.dial :waste1 @21:03:55.22 delay 30 seconds @21:04:25.24 PBV.dial between PSV and waste1 @21:04:25.60 PSR.select :air @21:04:25.62 PSV.dial PSR @21:04:26.39 PS.reconfigure :fast @21:04:26.44 PS.seek 2.5ml @21:04:50.17 PSV.dial PTV @21:04:50.94 delay 5 seconds @21:04:55.96 PSV.dial PSR @21:04:56.72 delay 5 seconds @21:05:01.73 PSV.dial PTV @21:05:02.51 delay 5 seconds @21:05:07.53 PSV.dial :air @21:05:08.41 PS.seek 0ml @21:05:31.67 PTV.dial :PRVmixing,via: PRV @21:05:32.99 PSV.dial :mixloop @21:05:33.45 PRV.dial :ab1 @21:05:34.20 PS.reconfigure :slow @21:05:34.25 PS.seek 0.15ml @21:05:38.04 delay 2 seconds @21:05:40.06 CBV.dial PRV @21:05:40.08
PRV.dial CBV @21:05:40.66 PS.seek 0.35ml @21:05:45.23 delay 2 seconds @21:05:47.25 CBV.dial between PRV and PSV @21:05:47.57 PRV.dial :air @21:05:48.47 PS.seek 0.9ml @21:06:00.38 PRV.dial :waste2 @21:06:01.14 PS.seek 0.5ml @21:06:09.82 addPMLReagent08 @21:06:09.83 PSV.dial :air @21:06:10.30 PS.reconfigure :default @21:06:10.34 PS.seek 2ml @21:06:26.54 PSV.dial :PML @21:06:27.01 PTV.dial :mixing @21:06:27.77 PBV.dial :waste1 @21:06:28.21 PS.seek 1.85ml @21:06:30.57 PBV.dial between PSV and waste1 @21:06:30.94 PS.seek 0ml @21:06:50.85 delay 2 seconds @21:06:52.87 Beginning samplePassC @21:06:52.88 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @21:06:53.07 Elbow.reconfigure :tight @21:06:53.12 Elbow.seek FlushPuck::Garage @21:06:54.47 Elbow.reconfigure :loose @21:06:54.57 Forearm.seek :garage @21:06:55.54 Hand.close @21:06:56.29 Forearm.seek :retract @21:06:57.29 Elbow.reconfigure :tight @21:06:57.34 Elbow.seek CC @21:07:00.26 Elbow.reconfigure :loose @21:07:00.36 Forearm.reconfigure :tight @21:07:00.41 Forearm.seek CC @21:07:01.64 CC.seek :closed @21:07:06.16 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.46 @21:07:06.23 Hand.open @21:07:06.87 Forearm.reconfigure :loose @21:07:06.91 Forearm.seek :retract @21:07:07.92 PSV.dial PSR @21:07:08.98 Pulling 1.5ml of PSR.flush @21:07:08.99 PSR.select :flush @21:07:09.11 PS.seek 1.5ml @21:07:25.29 delay 5 seconds @21:07:30.31 PSV.dial :waste1 @21:07:30.78 PS.seek 0ml @21:07:46.94 PSV.dial PSR @21:07:47.41 PSR.select :air @21:07:47.53 PS.seek 2.5ml @21:08:13.86 PRV.dial CBV,avoiding: :ab2 @21:08:13.88
CBV.dial PRV @21:08:15.51 PSV.dial :mixloop @21:08:16.56 CTV.dial :air,avoiding: :intake @21:08:16.63 PTV.dial :PRVmixing @21:08:17.40 PS.seek 0ml @21:08:43.79 PSV.dial :air @21:08:44.26 PS.seek 2.5ml @21:09:10.57 PSV.dial :mixloop @21:09:11.04 PS.seek 0ml @21:09:37.35 PSV.dial :air @21:09:37.95 PS.seek 2.5ml @21:10:04.35 PSV.dial :mixloop @21:10:04.93 PS.seek 0ml @21:10:31.35 Garaging FlushPuck in Collection Clamp @21:10:31.42 Elbow.reconfigure :tight @21:10:31.47 Elbow.seek CC @21:10:31.77 Elbow.reconfigure :loose @21:10:31.87 Forearm.seek CC @21:10:32.90 Hand.close @21:10:33.54 CC.seek :open @21:10:38.15 Forearm.seek :retract @21:10:39.24 Elbow.reconfigure :tight @21:10:39.67 Elbow.seek :garage @21:10:42.64 Elbow.reconfigure :loose @21:10:42.75 Forearm.reconfigure :tight @21:10:42.79 Forearm.seek :garage @21:10:43.82 Hand.open @21:10:44.47 Forearm.reconfigure :loose @21:10:44.52 Forearm.seek :retract @21:10:45.54 delayUntil "21:14:52.86" @21:10:45.57 Whole Cell Archive sampling 1000ml @21:10:45.59 Loading wcr puck from tube 4 into Collection Clamp @21:10:46.07 Elbow.reconfigure :tight @21:10:46.12 Elbow.seek 4 @21:10:48.13 Forearm.reconfigure :tight @21:10:48.18 Forearm.seek 4 + 450 counts @21:10:49.27 SE.reconfigure :pick @21:10:49.33 SE.seek :beamBreak @21:10:50.38 SE.configure :default @21:10:50.55 Elbow.seek 4 @21:10:50.74 Forearm.seek 4 + 300 counts @21:10:50.99 Forearm.reconfigure :loose @21:10:51.04 Hand.close @21:10:51.68 SE.seek :down @21:10:51.88 Forearm.seek :retract @21:10:52.38 SE.stop @21:10:52.82 Elbow.seek CC @21:10:54.92 Elbow.reconfigure :loose @21:10:55.04 Forearm.reconfigure :tight @21:10:55.09 Forearm.seek CC @21:10:56.28 CC.seek :closed @21:11:00.82 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.35 @21:11:00.83 Hand.open @21:11:01.48 Forearm.reconfigure :loose @21:11:01.53 Forearm.seek :retract @21:11:02.50 collectSample 1000ml w/bubble point of 35psi @21:11:02.51 Priming 7ml @21:11:02.52 Intake.select :close @21:11:02.53 Exhaust.select :close @21:11:02.54 SS.hold @21:11:02.76 Calibrating pressure sensors @21:11:02.77 CTV.dial :air @21:11:02.85 SSV.dial :spit,avoiding: :exhaust @21:11:02.94 SS.reconfigure :fast @21:11:02.99 SS.seek 5ml @21:11:05.70 SSV.dial CBV @21:11:05.72 CBV.dial SSV @21:11:07.11 delay 3.8 seconds @21:11:11.04 Sensor limits: Intake max 107.0psia; Outlet max 97.8psia @21:11:11.05 SSV.dial :spit @21:11:12.38 SS.seek 0ml @21:11:15.23 SSV.dial CBV @21:11:16.58 CTV.dial :intake @21:11:17.35 SSV.dial CBV @21:11:17.37 CBV.dial SSV @21:11:17.46 Allowing 40s for 7.0ml of remaining 7.0ml @21:11:17.47 Intake.select :open @21:11:19.64 Intake @14.2psia, vacuum from 8.7 to 11.7psi @21:11:19.66 SS.reconfigure :inhaling 2.20ml/s @21:11:19.71 SS.seek 7ml @21:11:27.53 Stroke completed -- waiting for pressure to equalize ... @21:11:27.54 SS.reconfigure :exhale @21:11:27.59 SS.hold @21:11:47.99 Intake.select :close @21:11:48.15 SSV.dial :exhaust @21:11:48.92 Exhaust.select :open @21:11:49.04 SS.seek 0ml @21:11:53.40 Exhaust.select :close @21:11:53.96 Sampling 1000.0ml @21:11:53.98 Intake.select :close @21:11:53.99 Exhaust.select :close @21:11:54.01 SS.reconfigure :default @21:11:54.05 SS.hold @21:11:54.26 CTV.dial :intake @21:11:54.51 SSV.dial CBV @21:11:54.53 CBV.dial SSV @21:11:55.33 Allowing 130s for 25.0ml of remaining 1000.0ml @21:11:55.34 Intake.select :open @21:11:57.51 Intake @15.3psia, vacuum from 9.8 to 12.8psi @21:11:57.53 SS.reconfigure :inhaling 2.20ml/s @21:11:57.58 SS.seek 25ml @21:12:04.30 Vacuum too high -- waiting up to 40 seconds @21:12:04.32 SS.reconfigure :exhale @21:12:04.37 SS.hold @21:12:13.31 Intake @17.8psia, vacuum from 10.0 to 13.0psi @21:12:13.32 SS.reconfigure :inhaling 1.76ml/s @21:12:13.37 SS.seek 25ml @21:12:16.74 Vacuum too high -- waiting up to 40 seconds @21:12:16.75 SS.reconfigure :exhale @21:12:16.81 SS.hold @21:12:20.35 Intake @18.1psia, vacuum from 10.0 to 13.0psi @21:12:20.37 SS.reconfigure :inhaling 1.41ml/s @21:12:20.41 SS.seek 25ml @21:12:23.46 Vacuum too high -- waiting up to 40 seconds @21:12:23.47 SS.reconfigure :exhale @21:12:23.53 SS.hold @21:12:26.69 Intake @18.0psia, vacuum from 10.0 to 13.0psi @21:12:26.70 SS.reconfigure :inhaling 1.13ml/s @21:12:26.75 SS.seek 25ml @21:12:30.30 Vacuum too high -- waiting up to 40 seconds @21:12:30.32 SS.reconfigure :exhale @21:12:30.37 SS.hold @21:12:33.07 Intake @18.0psia, vacuum from 10.0 to 13.0psi @21:12:33.09 SS.reconfigure :inhaling 0.91ml/s @21:12:33.14 SS.seek 25ml @21:12:37.49 Vacuum too high -- waiting up to 40 seconds @21:12:37.50 SS.reconfigure :exhale @21:12:37.56 SS.hold @21:12:40.13 Intake @18.1psia, vacuum from 10.0 to 13.0psi @21:12:40.14 SS.reconfigure :inhaling 0.73ml/s @21:12:40.19 SS.seek 25ml @21:12:41.68 Stroke completed -- waiting for pressure to equalize ... @21:12:41.69 SS.reconfigure :exhale @21:12:41.74 SS.hold @21:13:12.47 Intake.select :close @21:13:12.62 SSV.dial :exhaust @21:13:13.38 Exhaust.select :open @21:13:13.55 SS.seek 0ml @21:13:22.42 Exhaust.select :close @21:13:22.52 SSV.dial CBV @21:13:22.57 CBV.dial SSV @21:13:23.33 Allowing 130s for 25.0ml of remaining 975.0ml @21:13:23.34 Intake.select :open @21:13:25.51 Intake @15.5psia, vacuum from 10.0 to 13.0psi @21:13:25.53 SS.reconfigure :inhaling 0.73ml/s @21:13:25.58 SS.seek 25ml @21:14:02.29 Stroke completed -- waiting for pressure to equalize ... @21:14:02.30 SS.reconfigure :exhale @21:14:02.35 SS.hold @21:14:33.10 Intake.select :close @21:14:33.25 SSV.dial :exhaust @21:14:34.02 Exhaust.select :open @21:14:34.14 SS.seek 0ml @21:14:43.07 Exhaust.select :close @21:14:43.18 SSV.dial CBV @21:14:43.22 CBV.dial SSV @21:14:43.98 Allowing 130s for 25.0ml of remaining 950.0ml @21:14:43.99 Intake.select :open @21:14:46.61 Intake @15.7psia, vacuum from 10.0 to 13.0psi @21:14:46.63 SS.reconfigure :inhaling 0.73ml/s @21:14:46.68 SS.seek 25ml @21:14:52.87 recoverPRVreagent08 @21:14:52.88 PTV.dial :mixing @21:14:53.64 PBV.dial :waste1 @21:14:54.02 delay 30 seconds @21:15:22.52 Stroke completed -- waiting for pressure to equalize ... @21:15:22.54 SS.reconfigure :exhale @21:15:22.58 SS.hold @21:15:24.03 PBV.dial between PSV and waste1 @21:15:24.40 PSV.dial :air @21:15:24.88 PS.reconfigure :fast @21:15:24.93 PS.seek 2.5ml @21:15:49.11 PSV.dial :mixloop @21:15:49.59 delay 2 seconds @21:15:51.28 Intake.select :close @21:15:51.44 SSV.dial :exhaust @21:15:51.61 PSV.dial :air @21:15:52.09 delay 2 seconds @21:15:52.23 Exhaust.select :open @21:15:52.34 SS.seek 0ml @21:15:54.11 PSV.dial :mixloop @21:15:54.59 delay 2 seconds @21:15:56.61 PSV.dial :air @21:15:57.10 PS.seek 0ml @21:16:01.40 Exhaust.select :close @21:16:01.51 SSV.dial CBV @21:16:01.54 CBV.dial SSV @21:16:02.30 Allowing 130s for 25.0ml of remaining 925.0ml @21:16:02.32 Intake.select :open @21:16:04.49 Intake @15.7psia, vacuum from 10.0 to 13.0psi @21:16:04.50 SS.reconfigure :inhaling 0.73ml/s @21:16:04.55 SS.seek 25ml @21:16:20.94 washCycleDA #1 of 2 @21:16:20.95 PSR.select :air @21:16:20.97 PSV.dial PSR @21:16:22.31 Pulling 0.5ml of PSR.wash @21:16:22.32 PSR.select :wash @21:16:22.44 PS.reconfigure :default @21:16:22.49 PS.seek 0.5ml @21:16:28.47 delay 5 seconds @21:16:33.49 PSR.select :air @21:16:33.61 PS.seek 2ml @21:16:40.91 Stroke completed -- waiting for pressure to equalize ... @21:16:40.92 SS.reconfigure :exhale @21:16:40.97 SS.hold @21:16:49.78 PTV.dial :puck @21:16:50.53 PSV.dial PTV @21:16:51.28 PBV.dial :waste1 @21:16:51.71 PS.seek 1.5ml @21:16:57.72 delay 3 seconds @21:17:00.74 PBV.dial between PSV and waste1 @21:17:01.13 PS.seek 0ml @21:17:05.96 Intake.select :close @21:17:06.12 SSV.dial :exhaust @21:17:06.90 Exhaust.select :open @21:17:07.01 SS.seek 0ml @21:17:16.08 Exhaust.select :close @21:17:16.19 SSV.dial CBV @21:17:16.21 CBV.dial SSV @21:17:16.99 Allowing 130s for 25.0ml of remaining 900.0ml @21:17:17.00 Intake.select :open @21:17:17.54 delay 2:00 @21:17:19.50 Intake @15.6psia, vacuum from 10.0 to 13.0psi @21:17:19.52 SS.reconfigure :inhaling 0.73ml/s @21:17:19.57 SS.seek 25ml @21:17:55.61 Stroke completed -- waiting for pressure to equalize ... @21:17:55.62 SS.reconfigure :exhale @21:17:55.67 SS.hold @21:18:18.13 Intake.select :close @21:18:18.67 SSV.dial :exhaust @21:18:19.50 Exhaust.select :open @21:18:19.62 SS.seek 0ml @21:18:28.78 Exhaust.select :close @21:18:28.89 SSV.dial CBV @21:18:28.91 CBV.dial SSV @21:18:29.69 Allowing 130s for 25.0ml of remaining 875.0ml @21:18:29.71 Intake.select :open @21:18:31.88 Intake @15.7psia, vacuum from 10.0 to 13.0psi @21:18:31.90 SS.reconfigure :inhaling 0.73ml/s @21:18:31.95 SS.seek 25ml @21:19:07.52 Stroke completed -- waiting for pressure to equalize ... @21:19:07.53 SS.reconfigure :exhale @21:19:07.58 SS.hold @21:19:17.56 recoverPSRreagent08 @21:19:17.57 PTV.dial :puck @21:19:17.65 PBV.dial :waste1 @21:19:18.03 delay 30 seconds @21:19:30.51 Intake.select :close @21:19:30.67 SSV.dial :exhaust @21:19:31.44 Exhaust.select :open @21:19:31.56 SS.seek 0ml @21:19:40.83 Exhaust.select :close @21:19:40.94 SSV.dial CBV @21:19:40.98 CBV.dial SSV @21:19:41.75 Allowing 130s for 25.0ml of remaining 850.0ml @21:19:41.77 Intake.select :open @21:19:43.94 Intake @15.5psia, vacuum from 10.0 to 13.0psi @21:19:43.95 SS.reconfigure :inhaling 0.73ml/s @21:19:44.00 SS.seek 25ml @21:19:48.05 PBV.dial between PSV and waste1 @21:19:48.42 PSR.select :air @21:19:48.43 PSV.dial PSR @21:19:49.20 PS.reconfigure :fast @21:19:49.25 PS.seek 2.5ml @21:20:13.40 PSV.dial PTV @21:20:14.18 delay 5 seconds @21:20:19.20 PSV.dial PSR @21:20:19.48 Stroke completed -- waiting for pressure to equalize ... @21:20:19.50 SS.reconfigure :exhale @21:20:19.55 SS.hold @21:20:19.98 delay 5 seconds @21:20:25.00 PSV.dial PTV @21:20:25.76 delay 5 seconds @21:20:30.78 PSV.dial :air @21:20:31.55 PS.seek 0ml @21:20:44.10 Intake.select :close @21:20:44.27 SSV.dial :exhaust @21:20:45.05 Exhaust.select :open @21:20:45.17 SS.seek 0ml @21:20:54.17 Exhaust.select :close @21:20:54.29 SSV.dial CBV @21:20:54.31 CBV.dial SSV @21:20:55.10 Allowing 130s for 25.0ml of remaining 825.0ml @21:20:55.14 washCycleDA #2 of 2 @21:20:55.15 Intake.select :open @21:20:55.16 PSR.select :air @21:20:55.28 PSV.dial PSR @21:20:56.62 Pulling 0.5ml of PSR.wash @21:20:56.63 PSR.select :wash @21:20:56.76 PS.reconfigure :default @21:20:56.80 PS.seek 0.5ml @21:20:57.37 Intake @15.5psia, vacuum from 10.0 to 13.0psi @21:20:57.39 SS.reconfigure :inhaling 0.73ml/s @21:20:57.44 SS.seek 25ml @21:21:02.84 delay 5 seconds @21:21:07.86 PSR.select :air @21:21:07.98 PS.seek 2ml @21:21:24.21 PTV.dial :puck @21:21:24.75 PSV.dial PTV @21:21:25.51 PBV.dial :waste1 @21:21:25.95 PS.seek 1.5ml @21:21:31.87 delay 3 seconds @21:21:33.55 Stroke completed -- waiting for pressure to equalize ... @21:21:33.56 SS.reconfigure :exhale @21:21:33.61 SS.hold @21:21:34.88 PBV.dial between PSV and waste1 @21:21:35.26 PS.seek 0ml @21:21:51.40 delay 2:00 @21:21:56.13 Intake.select :close @21:21:56.29 SSV.dial :exhaust @21:21:57.08 Exhaust.select :open @21:21:57.20 SS.seek 0ml @21:22:05.92 Exhaust.select :close @21:22:06.04 SSV.dial CBV @21:22:06.06 CBV.dial SSV @21:22:06.83 Allowing 130s for 25.0ml of remaining 800.0ml @21:22:06.84 Intake.select :open @21:22:09.01 Intake @15.5psia, vacuum from 10.0 to 13.0psi @21:22:09.03 SS.reconfigure :inhaling 0.73ml/s @21:22:09.08 SS.seek 25ml @21:22:45.31 Stroke completed -- waiting for pressure to equalize ... @21:22:45.32 SS.reconfigure :exhale @21:22:45.37 SS.hold @21:23:07.94 Intake.select :close @21:23:08.10 SSV.dial :exhaust @21:23:08.86 Exhaust.select :open @21:23:08.98 SS.seek 0ml @21:23:17.69 Exhaust.select :close @21:23:17.80 SSV.dial CBV @21:23:17.82 CBV.dial SSV @21:23:18.59 Allowing 130s for 25.0ml of remaining 775.0ml @21:23:18.61 Intake.select :open @21:23:20.77 Intake @15.6psia, vacuum from 10.0 to 13.0psi @21:23:20.79 SS.reconfigure :inhaling 0.73ml/s @21:23:20.84 SS.seek 25ml @21:23:51.43 recoverPSRreagent08 @21:23:51.44 PTV.dial :puck @21:23:51.51 PBV.dial :waste1 @21:23:51.88 delay 30 seconds @21:23:57.36 Stroke completed -- waiting for pressure to equalize ... @21:23:57.37 SS.reconfigure :exhale @21:23:57.42 SS.hold @21:24:21.94 PBV.dial between PSV and waste1 @21:24:22.06 Intake.select :close @21:24:22.22 SSV.dial :exhaust @21:24:22.36 PSR.select :air @21:24:22.38 PSV.dial PSR @21:24:23.00 Exhaust.select :open @21:24:23.12 SS.seek 0ml @21:24:23.20 PS.reconfigure :fast @21:24:23.25 PS.seek 2.5ml @21:24:32.18 Exhaust.select :close @21:24:32.29 SSV.dial CBV @21:24:32.33 CBV.dial SSV @21:24:33.09 Allowing 130s for 25.0ml of remaining 750.0ml @21:24:33.11 Intake.select :open @21:24:35.27 Intake @15.5psia, vacuum from 10.0 to 13.0psi @21:24:35.29 SS.reconfigure :inhaling 0.73ml/s @21:24:35.34 SS.seek 25ml @21:24:47.46 PSV.dial PTV @21:24:48.22 delay 5 seconds @21:24:53.25 PSV.dial PSR @21:24:54.01 delay 5 seconds @21:24:59.03 PSV.dial PTV @21:24:59.80 delay 5 seconds @21:25:04.82 PSV.dial :air @21:25:05.60 PS.seek 0ml @21:25:11.45 Stroke completed -- waiting for pressure to equalize ... @21:25:11.46 SS.reconfigure :exhale @21:25:11.51 SS.hold @21:25:28.90 getPMLReagent08 @21:25:28.91 PRV.dial :air @21:25:29.37 delay 5 seconds @21:25:34.38 PTV.dial :PRVmixing @21:25:35.71 delay 5 seconds @21:25:38.36 Intake.select :close @21:25:38.52 SSV.dial :exhaust @21:25:39.37 Exhaust.select :open @21:25:39.49 SS.seek 0ml @21:25:40.73 PRV.dial :ab2 @21:25:41.18 PTV.dial :PRVmixing @21:25:41.19 PSV.dial :mixloop @21:25:41.68 PS.reconfigure :default @21:25:41.73 PS.seek 0.3ml @21:25:45.61 delay 2 seconds @21:25:47.63 PRV.dial :air @21:25:48.16 PS.seek 0.55ml @21:25:48.39 Exhaust.select :close @21:25:48.50 SSV.dial CBV @21:25:48.53 CBV.dial SSV @21:25:49.29 Allowing 130s for 25.0ml of remaining 725.0ml @21:25:49.31 Intake.select :open @21:25:51.48 Intake @15.5psia, vacuum from 10.0 to 13.0psi @21:25:51.49 SS.reconfigure :inhaling 0.73ml/s @21:25:51.54 SS.seek 25ml @21:25:51.62 addPMLReagent08 @21:25:51.63 PSV.dial :air @21:25:52.10 PS.seek 2ml @21:26:07.71 PSV.dial :PML @21:26:08.18 PTV.dial :mixing @21:26:08.96 PBV.dial :waste1 @21:26:09.39 PS.seek 1.85ml @21:26:11.71 PBV.dial between PSV and waste1 @21:26:12.09 PS.seek 0ml @21:26:27.85 Stroke completed -- waiting for pressure to equalize ... @21:26:27.87 SS.reconfigure :exhale @21:26:27.91 SS.hold @21:26:31.77 delay 2 seconds @21:26:33.79 delay 8:00 @21:26:54.57 Intake.select :close @21:26:54.73 SSV.dial :exhaust @21:26:55.50 Exhaust.select :open @21:26:55.62 SS.seek 0ml @21:27:04.56 Exhaust.select :close @21:27:04.67 SSV.dial CBV @21:27:04.69 CBV.dial SSV @21:27:05.48 Allowing 130s for 25.0ml of remaining 700.0ml @21:27:05.50 Intake.select :open @21:27:07.66 Intake @15.5psia, vacuum from 10.0 to 13.0psi @21:27:07.68 SS.reconfigure :inhaling 0.73ml/s @21:27:07.73 SS.seek 25ml @21:27:43.68 Stroke completed -- waiting for pressure to equalize ... @21:27:43.69 SS.reconfigure :exhale @21:27:43.74 SS.hold @21:28:14.61 Intake.select :close @21:28:14.77 SSV.dial :exhaust @21:28:15.54 Exhaust.select :open @21:28:15.66 SS.seek 0ml @21:28:24.43 Exhaust.select :close @21:28:24.54 SSV.dial CBV @21:28:24.57 CBV.dial SSV @21:28:25.34 Allowing 130s for 25.0ml of remaining 675.0ml @21:28:25.75 Intake.select :open @21:28:27.98 Intake @15.4psia, vacuum from 9.9 to 12.9psi @21:28:28.01 SS.reconfigure :inhaling 0.73ml/s @21:28:28.06 SS.seek 25ml @21:29:04.35 Stroke completed -- waiting for pressure to equalize ... @21:29:04.36 SS.reconfigure :exhale @21:29:04.41 SS.hold @21:29:37.11 Intake.select :close @21:29:37.27 SSV.dial :exhaust @21:29:38.05 Exhaust.select :open @21:29:38.17 SS.seek 0ml @21:29:46.96 Exhaust.select :close @21:29:47.08 SSV.dial CBV @21:29:47.10 CBV.dial SSV @21:29:47.90 Allowing 130s for 25.0ml of remaining 650.0ml @21:29:47.91 Intake.select :open @21:29:50.08 Intake @15.3psia, vacuum from 9.8 to 12.8psi @21:29:50.10 SS.reconfigure :inhaling 0.73ml/s @21:29:50.15 SS.seek 25ml @21:30:26.44 Stroke completed -- waiting for pressure to equalize ... @21:30:26.45 SS.reconfigure :exhale @21:30:26.50 SS.hold @21:31:01.29 Intake.select :close @21:31:01.45 SSV.dial :exhaust @21:31:02.21 Exhaust.select :open @21:31:02.33 SS.seek 0ml @21:31:11.43 Exhaust.select :close @21:31:11.54 SSV.dial CBV @21:31:11.56 CBV.dial SSV @21:31:12.34 Allowing 130s for 25.0ml of remaining 625.0ml @21:31:12.35 Intake.select :open @21:31:14.52 Intake @15.3psia, vacuum from 9.8 to 12.8psi @21:31:14.54 SS.reconfigure :inhaling 0.73ml/s @21:31:14.59 SS.seek 25ml @21:31:43.22 Vacuum too high -- waiting up to 40 seconds @21:31:43.23 SS.reconfigure :exhale @21:31:43.29 SS.hold @21:32:02.28 Intake @16.4psia, vacuum from 10.0 to 13.0psi @21:32:02.29 SS.reconfigure :inhaling 0.59ml/s @21:32:02.34 SS.seek 25ml @21:32:11.70 Stroke completed -- waiting for pressure to equalize ... @21:32:11.72 SS.reconfigure :exhale @21:32:11.77 SS.hold @21:32:30.17 Intake.select :close @21:32:30.33 SSV.dial :exhaust @21:32:31.10 Exhaust.select :open @21:32:31.22 SS.seek 0ml @21:32:39.99 Exhaust.select :close @21:32:40.10 SSV.dial CBV @21:32:40.12 CBV.dial SSV @21:32:40.90 Allowing 130s for 25.0ml of remaining 600.0ml @21:32:40.91 Intake.select :open @21:32:43.08 Intake @15.4psia, vacuum from 9.9 to 12.9psi @21:32:43.10 SS.reconfigure :inhaling 0.59ml/s @21:32:43.15 SS.seek 25ml @21:33:24.78 Vacuum too high -- waiting up to 40 seconds @21:33:24.79 SS.reconfigure :exhale @21:33:24.85 SS.hold @21:33:45.71 Intake @17.2psia, vacuum from 10.0 to 13.0psi @21:33:45.73 SS.reconfigure :inhaling 0.47ml/s @21:33:45.78 SS.seek 25ml @21:33:50.36 Stroke completed -- waiting for pressure to equalize ... @21:33:50.37 SS.reconfigure :exhale @21:33:50.42 SS.hold @21:34:04.88 Intake.select :close @21:34:05.04 SSV.dial :exhaust @21:34:05.81 Exhaust.select :open @21:34:05.93 SS.seek 0ml @21:34:14.97 Exhaust.select :close @21:34:15.08 SSV.dial CBV @21:34:15.11 CBV.dial SSV @21:34:15.88 Allowing 130s for 25.0ml of remaining 575.0ml @21:34:15.89 Intake.select :open @21:34:18.06 Intake @15.4psia, vacuum from 9.9 to 12.9psi @21:34:18.08 SS.reconfigure :inhaling 0.47ml/s @21:34:18.13 SS.seek 25ml @21:34:33.81 recoverPRVreagent08 @21:34:33.82 PTV.dial :mixing @21:34:33.99 PBV.dial :waste1 @21:34:34.37 delay 30 seconds @21:35:04.40 PBV.dial between PSV and waste1 @21:35:04.77 PSV.dial :air @21:35:05.25 PS.reconfigure :fast @21:35:05.30 PS.seek 2.5ml @21:35:13.48 Stroke completed -- waiting for pressure to equalize ... @21:35:13.50 SS.reconfigure :exhale @21:35:13.54 SS.hold @21:35:29.35 PSV.dial :mixloop @21:35:29.82 delay 2 seconds @21:35:31.84 PSV.dial :air @21:35:32.31 delay 2 seconds @21:35:34.32 PSV.dial :mixloop @21:35:34.79 delay 2 seconds @21:35:36.81 PSV.dial :air @21:35:37.28 PS.seek 0ml @21:35:44.40 Intake.select :close @21:35:44.96 SSV.dial :exhaust @21:35:45.80 Exhaust.select :open @21:35:45.92 SS.seek 0ml @21:35:54.70 Exhaust.select :close @21:35:54.81 SSV.dial CBV @21:35:54.84 CBV.dial SSV @21:35:55.63 Allowing 130s for 25.0ml of remaining 550.0ml @21:35:55.64 Intake.select :open @21:35:57.81 Intake @15.5psia, vacuum from 10.0 to 13.0psi @21:35:57.83 SS.reconfigure :inhaling 0.47ml/s @21:35:57.88 SS.seek 25ml @21:36:00.95 washCycleDA #1 of 3 @21:36:00.96 PSR.select :air @21:36:00.97 PSV.dial PSR @21:36:02.32 Pulling 0.5ml of PSR.wash @21:36:02.33 PSR.select :wash @21:36:02.45 PS.reconfigure :default @21:36:02.50 PS.seek 0.5ml @21:36:08.48 delay 5 seconds @21:36:13.50 PSR.select :air @21:36:13.62 PS.seek 2ml @21:36:29.85 PTV.dial :puck @21:36:30.62 PSV.dial PTV @21:36:31.37 PBV.dial :waste1 @21:36:31.80 PS.seek 1.5ml @21:36:37.73 delay 3 seconds @21:36:40.75 PBV.dial between PSV and waste1 @21:36:41.12 PS.seek 0ml @21:36:53.65 Stroke completed -- waiting for pressure to equalize ... @21:36:53.67 SS.reconfigure :exhale @21:36:53.71 SS.hold @21:36:57.26 delay 2:00 @21:37:26.46 Intake.select :close @21:37:26.61 SSV.dial :exhaust @21:37:27.39 Exhaust.select :open @21:37:27.51 SS.seek 0ml @21:37:36.57 Exhaust.select :close @21:37:36.68 SSV.dial CBV @21:37:36.72 CBV.dial SSV @21:37:37.48 Allowing 130s for 25.0ml of remaining 525.0ml @21:37:37.50 Intake.select :open @21:37:39.66 Intake @15.5psia, vacuum from 10.0 to 13.0psi @21:37:39.68 SS.reconfigure :inhaling 0.47ml/s @21:37:39.73 SS.seek 25ml @21:38:36.31 Stroke completed -- waiting for pressure to equalize ... @21:38:36.32 SS.reconfigure :exhale @21:38:36.37 SS.hold @21:38:57.28 recoverPSRreagent08 @21:38:57.29 PTV.dial :puck @21:38:57.30 PBV.dial :waste1 @21:38:57.68 delay 30 seconds @21:39:11.95 Intake.select :close @21:39:12.11 SSV.dial :exhaust @21:39:12.89 Exhaust.select :open @21:39:13.01 SS.seek 0ml @21:39:21.76 Exhaust.select :close @21:39:21.87 SSV.dial CBV @21:39:21.92 CBV.dial SSV @21:39:22.67 Allowing 130s for 25.0ml of remaining 500.0ml @21:39:22.68 Intake.select :open @21:39:24.85 Intake @15.5psia, vacuum from 10.0 to 13.0psi @21:39:24.87 SS.reconfigure :inhaling 0.47ml/s @21:39:24.92 SS.seek 25ml @21:39:27.70 PBV.dial between PSV and waste1 @21:39:28.07 PSR.select :air @21:39:28.09 PSV.dial PSR @21:39:28.87 PS.reconfigure :fast @21:39:28.92 PS.seek 2.5ml @21:39:53.00 PSV.dial PTV @21:39:53.79 delay 5 seconds @21:39:58.81 PSV.dial PSR @21:39:59.58 delay 5 seconds @21:40:04.60 PSV.dial PTV @21:40:05.36 delay 5 seconds @21:40:10.39 PSV.dial :air @21:40:11.17 PS.seek 0ml @21:40:20.57 Stroke completed -- waiting for pressure to equalize ... @21:40:20.58 SS.reconfigure :exhale @21:40:20.63 SS.hold @21:40:34.63 washCycleDA #2 of 3 @21:40:34.64 PSR.select :air @21:40:34.66 PSV.dial PSR @21:40:36.00 Pulling 0.5ml of PSR.wash @21:40:36.01 PSR.select :wash @21:40:36.13 PS.reconfigure :default @21:40:36.18 PS.seek 0.5ml @21:40:42.07 delay 5 seconds @21:40:47.09 PSR.select :air @21:40:47.28 PS.seek 2ml @21:41:00.82 SS.reconfigure :default @21:41:00.87 SS.hold @21:41:00.94 Intake.select :close @21:41:01.10 ShallowSampler::Clogged in WCR -- Intake @16psia & Outlet @14psia won't equalize after 525.0ml @21:41:01.11 SSV.dial :exhaust @21:41:01.87 Exhaust.select :open @21:41:01.99 SS.reconfigure :exhale @21:41:02.51 SS.seek 0ml @21:41:03.41 PTV.dial :puck @21:41:03.42 PSV.dial PTV @21:41:04.30 PBV.dial :waste1 @21:41:04.76 PS.seek 1.5ml @21:41:10.72 delay 3 seconds @21:41:11.60 Exhaust.select :close @21:41:11.71 Sampled 525.0ml @21:41:11.73 Drying puck before fixing for 5:00 @21:41:11.74 CBV.dial :waste2b @21:41:12.51 delay 1:00 @21:41:13.74 PBV.dial between PSV and waste1 @21:41:14.12 PS.seek 0ml @21:41:30.28 delay 2:00 @21:42:12.53 CSV.dial :air @21:42:12.98 CS.reconfigure :fast @21:42:13.03 CS.seek 10ml @21:42:37.76 Pumping wcr puck from tube 4 up to 35psi... @21:42:37.78 CBV.dial SSV @21:42:39.04 CBV.dial between CSV and waste2b @21:42:40.02 CSV.dial between air and CTV @21:42:40.34 CS.seek 7.372ml @21:42:47.14 CTV.dial CSV,avoiding: :air @21:42:47.18 CSV.dial CTV @21:42:48.15 CS.reconfigure :pressurize @21:42:48.21 CS.seek 0ml @21:43:07.00 CBV.dial :waste2b @21:43:07.32 CS.reconfigure :default @21:43:07.38 CS.seek 0ml @21:43:08.60 delay 1:00 @21:43:30.31 recoverPSRreagent08 @21:43:30.33 PTV.dial :puck @21:43:30.34 PBV.dial :waste1 @21:43:30.70 delay 30 seconds @21:44:00.73 PBV.dial between PSV and waste1 @21:44:01.11 PSR.select :air @21:44:01.13 PSV.dial PSR @21:44:01.90 PS.reconfigure :fast @21:44:01.94 PS.seek 2.5ml @21:44:08.62 CTV.dial :air,avoiding: :intake @21:44:10.01 Fix #1 of 2 (with RNAlater) for 2:30 @21:44:10.03 CSV.dial CSR @21:44:10.46 Pulling 2ml of CSR.RNAlater @21:44:10.47 CSR.select :RNAlater @21:44:10.59 CS.seek 2ml @21:44:16.57 delay 5 seconds @21:44:21.59 CSR.select :air @21:44:21.71 CS.reconfigure :fast @21:44:22.22 CS.seek 10ml @21:44:26.09 PSV.dial PTV @21:44:26.87 delay 5 seconds @21:44:31.89 PSV.dial PSR @21:44:32.66 delay 5 seconds @21:44:37.68 PSV.dial PTV @21:44:38.58 delay 5 seconds @21:44:42.26 Pumping wcr puck from tube 4 up to 35psi... @21:44:42.28 CBV.dial SSV @21:44:43.54 CBV.dial between CSV and waste2b @21:44:43.60 PSV.dial :air @21:44:44.38 PS.seek 0ml @21:44:44.53 CSV.dial between air and CTV @21:44:45.14 CS.seek 7.288ml @21:44:52.29 CTV.dial CSV,avoiding: :air @21:44:52.32 CSV.dial CTV @21:44:53.73 CS.reconfigure :pressurize @21:44:53.78 CS.seek 0ml @21:45:07.81 delay 2:30 @21:45:07.97 washCycleDA #3 of 3 @21:45:07.98 PSR.select :air @21:45:08.00 PSV.dial PSR @21:45:09.34 Pulling 0.5ml of PSR.wash @21:45:09.35 PSR.select :wash @21:45:09.47 PS.reconfigure :default @21:45:09.52 PS.seek 0.5ml @21:45:15.40 delay 5 seconds @21:45:20.43 PSR.select :air @21:45:20.55 PS.seek 2ml @21:45:36.65 PTV.dial :puck @21:45:36.67 PSV.dial PTV @21:45:37.42 PBV.dial :waste1 @21:45:37.85 PS.seek 1.5ml @21:45:43.75 delay 3 seconds @21:45:46.77 PBV.dial between PSV and waste1 @21:45:47.14 PS.seek 0ml @21:46:03.25 delay 2:00 @21:47:37.83 CBV.dial :waste2b @21:47:38.18 CS.reconfigure :default @21:47:38.23 CS.seek 0ml @21:47:44.06 delay 1:00 @21:48:03.28 recoverPSRreagent08 @21:48:03.29 PTV.dial :puck @21:48:03.30 PBV.dial :waste1 @21:48:03.67 delay 30 seconds @21:48:33.69 PBV.dial between PSV and waste1 @21:48:34.07 PSR.select :air @21:48:34.09 PSV.dial PSR @21:48:34.86 PS.reconfigure :fast @21:48:34.91 PS.seek 2.5ml @21:48:44.09 CTV.dial :air,avoiding: :intake @21:48:45.49 Fix #2 of 2 (with RNAlater) for 2:30 @21:48:45.50 CSV.dial CSR @21:48:45.95 Pulling 2ml of CSR.RNAlater @21:48:45.97 CSR.select :RNAlater @21:48:46.09 CS.seek 2ml @21:48:52.25 delay 5 seconds @21:48:57.27 CSR.select :air @21:48:57.39 CS.reconfigure :fast @21:48:57.44 CS.seek 10ml @21:48:58.96 PSV.dial PTV @21:48:59.73 delay 5 seconds @21:49:04.75 PSV.dial PSR @21:49:05.51 delay 5 seconds @21:49:10.53 PSV.dial PTV @21:49:11.29 delay 5 seconds @21:49:16.31 PSV.dial :air @21:49:17.08 PS.seek 0ml @21:49:17.25 Pumping wcr puck from tube 4 up to 35psi... @21:49:17.27 CBV.dial SSV @21:49:18.54 CBV.dial between CSV and waste2b @21:49:19.51 CSV.dial between air and CTV @21:49:20.12 CS.seek 7.209ml @21:49:27.35 CTV.dial CSV,avoiding: :air @21:49:27.37 CSV.dial CTV @21:49:28.76 CS.reconfigure :pressurize @21:49:28.81 CS.seek 0ml @21:49:40.55 Gate.power :camera,:ON @21:49:40.57 Mixing DA Substrates @21:49:40.58 PSR.select :air @21:49:40.60 PSV.dial PSR @21:49:42.02 Pulling 0.25ml of PSR.sub1 @21:49:42.06 PSR.select :sub1 @21:49:42.27 PS.reconfigure :default @21:49:42.38 PS.seek 0.25ml @21:49:45.81 delay 5 seconds @21:49:45.84 delay 2:30 @21:49:50.86 Pulling 0.25ml of PSR.sub2 @21:49:50.87 PSR.select :sub2 @21:49:50.99 PS.seek 0.5ml @21:49:54.34 delay 5 seconds @21:49:59.36 PSR.select :air @21:49:59.47 PS.seek 2ml @21:50:15.68 PTV.dial :puck @21:50:15.69 PSV.dial PTV @21:50:16.46 PBV.dial :waste1 @21:50:16.91 PS.seek 1.5ml @21:50:22.87 delay 3 seconds @21:50:25.89 PBV.dial between PSV and waste1 @21:50:26.28 PS.seek 0ml @21:50:42.46 delay 10 seconds @21:50:52.49 recoverPSRreagent08 @21:50:52.50 PTV.dial :puck @21:50:52.51 PBV.dial :waste1 @21:50:52.88 delay 30 seconds @21:51:22.91 PBV.dial between PSV and waste1 @21:51:23.77 PSR.select :air @21:51:23.79 PSV.dial PSR @21:51:24.58 PS.reconfigure :fast @21:51:24.63 PS.seek 2.5ml @21:51:48.99 PSV.dial PTV @21:51:49.77 delay 5 seconds @21:51:54.80 PSV.dial PSR @21:51:55.59 delay 5 seconds @21:52:00.61 PSV.dial PTV @21:52:01.38 delay 5 seconds @21:52:06.40 PSV.dial :air @21:52:07.18 PS.seek 0ml @21:52:15.88 CBV.dial :waste2b @21:52:16.22 CS.reconfigure :default @21:52:16.27 CS.seek 0ml @21:52:19.18 delay 1:00 @21:52:31.24 Additional air clear @21:52:31.25 PSR.select :air @21:52:31.27 PSV.dial PSR @21:52:32.63 Pulling 2ml of PSR.air @21:52:32.64 PSR.select :air @21:52:32.66 PS.reconfigure :default @21:52:32.71 PS.seek 2ml @21:52:53.93 delay 5 seconds @21:52:58.95 PTV.dial :puck @21:52:58.97 PSV.dial PTV @21:52:59.72 PBV.dial :waste1 @21:53:00.16 PS.seek 1.5ml @21:53:06.08 delay 3 seconds @21:53:09.10 PBV.dial between PSV and waste1 @21:53:09.48 delay 1 second @21:53:10.49 PSV.dial :waste1 @21:53:11.58 PS.seek 0ml @21:53:19.21 CTV.dial :air,avoiding: :intake @21:53:27.82 Processing Puck Heater at 28C before imaging @21:53:27.83 PH.coast @21:53:27.95 Imaging da puck in Processing Clamp while storing it to tube 3 @21:53:28.13 SC.reconfigure :tight @21:53:28.17 SC.seek 3 @21:53:31.75 SC.configure :limp @21:53:31.88 SE.reconfigure :place @21:53:32.02 SE.seek :beamBreak @21:53:32.11 Elbow.reconfigure :tight @21:53:32.16 Elbow.seek PC @21:53:34.85 SE.configure :default @21:53:34.90 SE.seek :place @21:53:35.86 Elbow.reconfigure :loose @21:53:35.96 Forearm.seek PC @21:53:36.93 Hand.close @21:53:37.93 PC.seek :open @21:53:42.82 Forearm.seek :retract @21:53:43.84 Elbow.reconfigure :tight @21:53:43.89 Elbow.seek 3 @21:53:46.51 Forearm.reconfigure :tight @21:53:46.56 Forearm.seek 3 + 450 counts @21:53:47.55 Hand.open @21:53:48.19 SE.seek -0.26 Pucks below Top Plate @21:53:48.49 SE.reconfigure :seal @21:53:48.54 SE.seek -1.22 Pucks below Top Plate @21:53:49.13 Lights, camera, ACTION! @21:53:49.16 PuckCamera: snap -bin=2,2 -auto /var/log/esp/da23nov2221h850ml.tif @21:54:08.60 Waiting >20 seconds for da to relinquish FlushPuck @21:54:10.95 SX HX9/16 (USB:04): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 18.492 seconds /var/log/esp/da23nov2221h850ml.tif: TIFF Upload Complete @21:54:10.99 PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da23nov2221h850ml15s.tif @21:54:26.63 SX HX9/16 (USB:04): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15 seconds /var/log/esp/midres/da23nov2221h850ml15s.tif: TIFF Upload Complete @21:54:26.65 PuckCamera: snap -bin=2,2 20.0 /var/log/esp/midres/da23nov2221h850ml20s.tif @21:54:47.35 SX HX9/16 (USB:04): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 20 seconds /var/log/esp/midres/da23nov2221h850ml20s.tif: TIFF Upload Complete @21:54:47.37 Gate.power :camera,:OFF @21:54:47.39 DAfans.email "18.492 sec auto-exposure",Subject: "da23nov2221h850ml images!" @21:54:47.90 Elbow.seek 3 @21:54:48.16 Forearm.seek 3 + 450 counts @21:54:48.24 SE.reconfigure :gently @21:54:48.31 SE.seek -0.51 Pucks below Top Plate @21:54:49.00 Elbow.reconfigure :limp @21:54:49.06 Forearm.reconfigure :loose @21:54:49.11 Forearm.seek 3 @21:54:49.38 Elbow.hold @21:54:49.45 Elbow.reconfigure :tight @21:54:49.51 SE.reconfigure :default @21:54:49.57 SE.seek -0.32 Pucks below Top Plate @21:54:50.01 Forearm.force -20 @21:54:50.06 Forearm.seek 3 + 318 counts @21:54:50.41 Forearm.reconfigure :tight @21:54:50.46 Forearm.seek 3 + 450 counts @21:54:51.58 SE.seek :down @21:54:52.87 Forearm.reconfigure :loose @21:54:52.97 Forearm.seek :retract @21:54:54.36 SE.stop @21:54:54.61 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @21:54:55.14 Elbow.seek FlushPuck::Garage @21:54:57.09 Elbow.reconfigure :loose @21:54:57.29 Forearm.seek :garage @21:54:58.46 Hand.close @21:54:59.39 Forearm.seek :retract @21:55:00.42 Elbow.reconfigure :tight @21:55:00.52 Elbow.seek PC @21:55:02.01 Elbow.reconfigure :loose @21:55:02.21 Forearm.reconfigure :tight @21:55:02.32 Forearm.seek PC @21:55:03.90 PC.seek :closed @21:55:08.72 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.59 @21:55:08.89 Hand.open @21:55:09.66 Forearm.reconfigure :loose @21:55:09.79 Forearm.seek :retract @21:55:10.87 PSR.select :air @21:55:10.93 Storing wcr puck in Collection Clamp to tube 3 @21:55:10.97 PSV.dial PSR @21:55:12.69 Pulling 0.5ml of PSR.flush @21:55:12.78 PSR.select :flush @21:55:13.23 PS.seek 0.5ml @21:55:13.38 Elbow.reconfigure :tight @21:55:13.48 Elbow.seek 3 @21:55:16.30 Forearm.reconfigure :tight @21:55:16.40 Forearm.seek 3 + 450 counts @21:55:17.52 SE.reconfigure :pick @21:55:17.64 SE.seek :beamBreak @21:55:19.39 delay 5 seconds @21:55:20.86 SE.configure :default @21:55:21.19 Elbow.reconfigure :limp @21:55:21.29 Forearm.reconfigure :loose @21:55:21.39 Forearm.seek 3 @21:55:21.71 Elbow.hold @21:55:21.86 Elbow.reconfigure :tight @21:55:22.07 SE.seek 0.17 Pucks below Top Plate @21:55:22.67 Forearm.force -20 @21:55:22.75 Forearm.seek 3 + 309 counts @21:55:23.15 Forearm.reconfigure :tight @21:55:23.22 Forearm.seek 3 + 450 counts @21:55:23.43 SE.seek 1.17 Pucks below Top Plate @21:55:23.67 upsync @21:55:23.92 Forearm.reconfigure :loose @21:55:23.97 Forearm.seek :retract @21:55:24.42 PSR.select :air @21:55:24.53 PS.seek 2.5ml @21:55:24.90 Elbow.seek CC @21:55:26.52 Elbow.reconfigure :loose @21:55:27.07 Forearm.seek CC @21:55:28.07 Hand.close @21:55:28.63 CC.seek :open @21:55:33.39 Forearm.seek :retract @21:55:34.35 Elbow.reconfigure :tight @21:55:34.40 Elbow.seek 3 @21:55:36.22 Elbow.reconfigure :limp @21:55:36.27 Forearm.seek 3 @21:55:37.32 Elbow.hold @21:55:37.38 Elbow.reconfigure :tight @21:55:37.43 Hand.open @21:55:38.12 Forearm.force -20 @21:55:38.18 Forearm.seek 3 + 308 counts @21:55:38.47 Forearm.reconfigure :tight @21:55:38.52 Forearm.seek 3 + 450 counts @21:55:38.77 SE.seek :down @21:55:39.91 Forearm.reconfigure :loose @21:55:39.96 Forearm.seek :retract @21:55:41.47 SE.stop @21:55:41.51
Starting finalPurge @21:55:45.86 delay 1 second @21:55:46.88 PRV.dial :waste2,avoiding: :ab2 @21:55:48.75 PTV.dial :PRVmixing @21:55:50.08 PSV.dial :mixloop @21:55:51.25 PS.seek :empty @21:56:01.47
Waiting >20 seconds for psFlush to relinquish FlushPuck @21:56:17.64 PSR.select :air @21:56:17.66 PSV.dial PSR @21:56:18.69 Pulling 0.5ml of PSR.flush @21:56:18.70 PSR.select :flush @21:56:18.82 PS.seek 0.5ml @21:56:25.17 delay 5 seconds @21:56:30.19 PSR.select :air @21:56:30.31 PS.seek 2.5ml @21:56:51.52 delay 1 second @21:56:52.54 PBV.dial :waste1 @21:56:52.91 PTV.dial :mixing @21:56:53.67 PSV.dial :mixloop @21:56:54.74 PS.seek :empty @21:57:21.11 PSV.dial PSR @21:57:22.16 PSR.select :flush @21:57:22.28 PS.seek 1ml @21:57:33.39 PSR.select :air @21:57:33.50 PS.seek 2.5ml @21:57:49.61 PSV.dial PTV @21:57:50.37 PTV.dial :puck @21:57:51.12 PBV.dial :waste1 @21:57:51.21 PS.seek 0ml @21:58:17.51 PSV.dial PSR @21:58:18.27 PSR.select :flush @21:58:18.39 PS.seek 0.5ml @21:58:24.39 PSR.select :air @21:58:24.51 PS.seek 2.5ml @21:58:45.70 PSV.dial PTV @21:58:46.58 PBV.dial PSV @21:58:47.15 PS.seek 0ml @21:59:13.51 PSV.dial PBV @21:59:13.97 PS.seek 2.5ml @21:59:40.39 PSV.dial :waste1 @21:59:41.14 PS.seek 0ml @22:00:07.51 PSV.dial PSR @22:00:07.98 PSR.select :air @22:00:08.01 PS.seek 2.5ml @22:00:34.42 PSV.dial PTV @22:00:35.28 PBV.dial PSV @22:00:35.36 PS.seek 0ml @22:01:01.73 PSV.dial PBV @22:01:02.19 PS.seek 2.5ml @22:01:28.51 PSV.dial :waste1 @22:01:29.27 PS.seek 0ml @22:01:55.63 PSV.dial PSR @22:01:56.10 PSR.select :flush @22:01:56.23 PS.seek 1ml @22:02:07.32 PSR.select :air @22:02:07.44 PS.seek 2.5ml @22:02:23.66 PSV.dial PTV @22:02:24.42 PTV.dial :puck @22:02:24.60 PBV.dial :waste1 @22:02:25.17 PS.seek 0ml @22:02:51.70 PSV.dial PSR @22:02:52.46 PSR.select :flush @22:02:52.59 PS.seek 0.5ml @22:02:58.48 PSR.select :air @22:02:58.59 PS.seek 2.5ml @22:03:19.91 PSV.dial PTV @22:03:20.68 PBV.dial PSV @22:03:21.25 PS.seek 0ml @22:03:47.60 PSV.dial PBV @22:03:48.08 PS.seek 2.5ml @22:04:14.41 PSV.dial :waste1 @22:04:15.17 PS.seek 0ml @22:04:41.76 PSV.dial PSR @22:04:42.22 PSR.select :air @22:04:42.25 PS.seek 2.5ml @22:05:08.66 PSV.dial PTV @22:05:09.40 PBV.dial PSV @22:05:09.59 PS.seek 0ml @22:05:36.00 PSV.dial PBV @22:05:36.53 PS.seek 2.5ml @22:06:02.85 PSV.dial :waste1 @22:06:03.59 PS.seek 0ml @22:06:29.98
Loading FlushPuck into Collection Clamp from Processing Clamp @22:06:30.16 Elbow.seek PC @22:06:32.66 Elbow.reconfigure :loose @22:06:32.76 Forearm.seek PC @22:06:33.71 Hand.close @22:06:34.37 PC.seek :open @22:06:39.09 Forearm.seek :retract @22:06:40.01 Elbow.reconfigure :tight @22:06:40.06 Elbow.seek CC @22:06:43.69 Elbow.reconfigure :loose @22:06:43.79 Forearm.reconfigure :tight @22:06:43.83 Forearm.seek CC @22:06:45.55 CC.seek :closed @22:06:50.08 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.53 @22:06:50.14 Hand.open @22:06:50.80 Forearm.reconfigure :loose @22:06:50.85 Forearm.seek :retract @22:06:51.87 Flushing the Collection Valve::Manifold @22:06:52.00 CSV.dial CSR @22:06:52.44 Pulling 5ml of CSR.flush CSR.select :flush @22:06:52.56 CS.seek 5ml @22:07:06.26 delay 5 seconds @22:07:11.28 CSR.select :air @22:07:11.45 CS.reconfigure :fast @22:07:11.50 CS.seek 7.5ml @22:07:18.26 CSV.dial :waste @22:07:19.56 CS.reconfigure :default @22:07:19.61 CS.seek 0ml @22:07:39.55 Flushing lower manifold's waste1 port @22:07:39.67 CSV.dial :flush @22:07:40.11 CS.reconfigure :fast @22:07:40.16 CS.seek 5ml @22:07:52.89 delay 5 seconds @22:07:57.91 CSV.dial :air @22:07:58.53 CS.seek 7.001ml @22:08:04.08 CSV.dial CTV @22:08:04.10
CTV.dial CSV @22:08:05.48 CBV.dial :waste1 @22:08:06.25 CS.seek 0ml @22:08:23.01 Flushing lower manifold's waste2 port @22:08:23.13 CSV.dial :flush @22:08:23.88 CS.seek 5ml @22:08:36.83 delay 5 seconds @22:08:41.84 CSV.dial :air @22:08:42.37 CS.seek 7ml @22:08:48.33 CSV.dial CTV @22:08:48.37
CTV.dial CSV @22:08:48.81 CBV.dial :waste2 @22:08:49.86 CS.seek 0ml @22:09:06.45 Flushing lower manifold's waste2b port @22:09:06.58 CSV.dial :flush @22:09:07.32 CS.seek 5ml @22:09:20.17 delay 5 seconds @22:09:25.19 CSV.dial :air @22:09:25.70 CS.seek 7ml @22:09:31.33 CSV.dial CTV @22:09:31.37
CTV.dial CSV @22:09:31.92 CBV.dial :waste2b @22:09:32.99 CS.seek 0ml @22:09:49.67 Disinfecting external Intake with kill @22:09:49.79 CSV.dial CSR @22:09:50.37 Pulling 8ml of CSR.kill @22:09:50.38 CSR.select :kill @22:09:50.49 CS.seek 8ml @22:10:10.42 delay 5 seconds @22:10:15.44 CSR.select :air @22:10:15.55 CS.seek 10ml @22:10:21.07 CSV.dial CBV @22:10:21.12
CBV.dial CSV @22:10:22.18 CTV.dial :intake @22:10:22.95 Intake.select :open @22:10:23.06 CS.seek 2ml @22:10:41.95 Intake.select :close @22:10:42.05 CTV.dial :air @22:10:42.81 CS.reconfigure :default @22:10:42.86 CS.seek 0ml @22:10:48.79 CSV.dial CSR @22:10:49.81 Pulling 5ml of CSR.flush @22:10:49.82 CSR.select :flush @22:10:49.93 CS.seek 5ml @22:11:03.88 delay 5 seconds @22:11:08.90 CSR.select :air @22:11:09.02 CS.seek 10ml @22:11:22.60 CSV.dial CBV @22:11:23.61 CS.seek 0ml @22:11:50.01 Garaging FlushPuck in Collection Clamp @22:11:50.08 Elbow.reconfigure :tight @22:11:50.13 Elbow.seek CC @22:11:50.38 Elbow.reconfigure :loose @22:11:50.47 Forearm.seek CC @22:11:51.52 Hand.close @22:11:52.12 CC.seek :open @22:11:57.19 Forearm.seek :retract @22:11:58.15 Elbow.reconfigure :tight @22:11:58.20 Elbow.seek :garage @22:12:01.17 Elbow.reconfigure :loose @22:12:01.27 Forearm.reconfigure :tight @22:12:01.32 Forearm.seek :garage @22:12:02.29 Hand.open @22:12:02.92 Forearm.reconfigure :loose @22:12:02.97 Forearm.seek :retract @22:12:03.91 Completed [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] begun 19:36:39 @22:12:03.97 Asleep after completing DA_WCR_112223 assays @22:12:03.99 GoodNews.email "{1 => 33,\n 2 => 33,\n 3 => 2,\n 4 => 31,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@22:05:48, 30% flow, 16.9C, 26% humidity, 15.2psia, 13.734V, 0.309A, 65.442Ah, 4.24W\nSampled 525.0ml",Subject: "Asleep after completing DA_WCR_112223 assays" @22:12:07.97 upsync + ESPwhich DA_WCR_112723.rb + fn=DA_WCR_112723.rb + IFS=: + set /home/esp/esp2/mission . + fullPath=/home/esp/esp2/mission/DA_WCR_112723.rb + [ -f /home/esp/esp2/mission/DA_WCR_112723.rb ] + realpath /home/esp/esp2/mission/DA_WCR_112723.rb + return 0 + scriptPath=/home/esp/esp2local/mission/DA_WCR_112723.rb + exec ruby /home/esp/esp2/lib/esp.rb /home/esp/esp2local/mission/DA_WCR_112723.rb @13:36:46.02UTC27-Nov-23
ESP2 v3.42 I2C Gateway v3.20 for core bus @13:36:48.70 Configuration for short puck jake 1/11/21 by brent@mbari.org /home/esp/esp2/type/shallow/jake/configure.rb @13:36:52.36 Polling Can every 10:00 @13:36:52.40 Cleanly exited at 13:30:52UTC27-Nov-23 @13:36:52.46 Loading /home/esp/esp2local/mission/DA_WCR_112723.rb mission @13:36:52.51 GoodNews.email "Starting DA_WCR_112723 w/pucks in tube 4\n{1 => 33,\n 2 => 33,\n 3 => 2,\n 4 => 31,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@13:36:52, 31% flow, 16.3C, 27% humidity, 15.1psia, 13.749V, 0.236A, 91.584Ah, 3.25W",Subject: "DA_WCR_112723 begun" @13:36:52.54
Preparing to sleep until + 00:00:05 @13:36:52.59 Asleep til + 00:00:05 @13:36:52.61 Continuing at 13:36 @13:36:52.89 Gate.power :core,:OFF @13:36:52.91 Gate.power :raw,:OFF @13:36:52.92 Shutdown @13:36:55.11 GoodNews.email "Then, in tube 4: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_112723.rb:14\n{1 => 33,\n 2 => 33,\n 3 => 2,\n 4 => 31,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@13:36:52, 31% flow, 16.3C, 27% humidity, 15.1psia, 13.749V, 0.248A, 91.584Ah, 3.41W",Subject: "Asleep until 13:36" @13:36:56.47 upsync @13:36:57.50
Powering core bus @13:36:58.99 upsync @13:37:03.29
Gate.power :raw,:ON @13:37:04.10 CS.configure :default @13:37:04.15 PS.configure :default @13:37:04.20 SS.configure :default @13:37:04.24 CC.configure :default @13:37:04.29 PC.configure :default @13:37:04.36 Forearm.configure :loose @13:37:04.40 Elbow.configure :loose @13:37:04.47 SC.configure :loose @13:37:04.52 SE.configure :default @13:37:04.57 CH.configure :default @13:37:04.60 PH.configure :default @13:37:04.64 All dwarf controllers running firmware version 3.85 @13:37:04.75 Valve.reset @13:37:05.02 Intake.select :close @13:37:05.05 Exhaust.select :close @13:37:05.06 CTV.dial :air @13:37:05.17 PSV.dial :air @13:37:05.21 CSV.dial :waste1 @13:37:05.32
Forearm.home.seek :retract @13:37:05.71 SSV.dial :spit,avoiding: :exhaust @13:37:06.07 Hand.close @13:37:06.10
Elbow.reconfigure :loose @13:37:06.19 Elbow.jog 1500 @13:37:06.29 PS.home.seek 0ml @13:37:06.59 CS.reconfigure :fast @13:37:06.62 SS.home.seek 0ml @13:37:06.76
Elbow.reconfigure :loose @13:37:06.77 CS.home.seek 0ml @13:37:06.87
Elbow.home.seek :clear @13:37:11.37 SE.home.seek :down @13:37:11.99 SC.reconfigure :tight @13:37:12.03 SC.home.seek :initial @13:37:14.62 PS.stop @13:37:40.39
SC.configure :limp @13:37:40.44 Forearm.seek :retract @13:37:40.51 Elbow.seek :hammer @13:37:41.62 Forearm.reconfigure :tight @13:37:41.67 Forearm.seek retracted @13:37:41.92 CC.home.seek :open @13:37:41.94 PC.home.seek :open @13:37:45.66
Phase [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] @13:37:45.67 Notice.email "At 13:37:45 began in tube 4: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_112723.rb:14\n{1 => 33,\n 2 => 33,\n 3 => 2,\n 4 => 31,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@13:37:45, 31% flow, 16.3C, 27% humidity, 15.1psia, 13.715V, 0.254A, 91.589Ah, 3.49W",Subject: "Started DA_WCR_112723 phase initialPurge; da [1000, 1000] #da and wsr are run simultanelously" @13:37:45.70
Starting initialPurge @13:37:45.72 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @13:37:45.78 Hand.open @13:37:46.56 Elbow.reconfigure :tight @13:37:46.63 Elbow.seek FlushPuck::Garage @13:37:48.59 Elbow.reconfigure :loose @13:37:48.71 Forearm.reconfigure :loose @13:37:48.76 Forearm.seek :garage @13:37:49.63 upsync @13:37:49.71
Hand.close @13:37:50.44 Forearm.seek :retract @13:37:51.46 Elbow.reconfigure :tight @13:37:51.51 Elbow.seek CC @13:37:54.44 Elbow.reconfigure :loose @13:37:54.54 Forearm.reconfigure :tight @13:37:54.59 Forearm.seek CC @13:37:55.94 CC.seek :closed @13:38:00.50 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.42 @13:38:00.58 Hand.open @13:38:01.25 Forearm.reconfigure :loose @13:38:01.30 Forearm.seek :retract @13:38:02.27 Flushing the Collection Valve::Manifold @13:38:02.40 CSV.dial CSR @13:38:03.70 Pulling 4.999ml of CSR.flush @13:38:03.71 CSR.select :flush @13:38:03.83 CS.reconfigure :default @13:38:04.28 CS.seek 5ml @13:38:17.92 delay 5 seconds @13:38:22.94 CSR.select :air @13:38:23.11 CS.reconfigure :fast @13:38:23.16 CS.seek 7.501ml @13:38:29.95 CSV.dial :waste @13:38:31.26 CS.reconfigure :default @13:38:31.30 CS.seek 0ml @13:38:51.52 Purging external Intake @13:38:51.64 CSV.dial :flush @13:38:52.08 CS.reconfigure :fast @13:38:52.13 CS.seek 10ml @13:39:16.61 delay 5 seconds @13:39:21.63 CSV.dial CBV @13:39:21.66
CBV.dial CSV @13:39:22.42 CTV.dial :intake @13:39:23.20 Intake.select :open @13:39:23.31 CS.seek 0ml @13:39:46.95 Intake.select :close @13:39:47.06 CTV.dial :air @13:39:47.85 Flushing upper manifold @13:39:47.97 CSV.dial :flush @13:39:48.71 CS.seek 5ml @13:40:01.61 delay 5 seconds @13:40:06.63 CSV.dial :air @13:40:07.14 CS.seek 7ml @13:40:12.70 CSV.dial CBV @13:40:12.73
CBV.dial CSV @13:40:13.78 CTV.dial :air @13:40:13.86 CS.seek 0ml @13:40:30.55 delay 7 seconds @13:40:37.57 CS.seek 3ml @13:40:45.61 CSV.dial :waste @13:40:46.07 CS.seek 0ml @13:40:53.81 CSV.dial :flush @13:40:54.27 CS.seek 3ml @13:41:02.51 delay 5 seconds @13:41:07.53 CSV.dial :air @13:41:08.03 CS.seek 5ml @13:41:13.57 CSV.dial :air @13:41:13.66 CS.seek 0ml @13:41:25.89 delay 7 seconds @13:41:32.92 CS.seek 3ml @13:41:40.98 CSV.dial :waste @13:41:41.72 CS.seek 0ml @13:41:49.67 Garaging FlushPuck in Collection Clamp @13:41:49.74 Elbow.reconfigure :tight @13:41:49.79 Elbow.seek CC @13:41:50.06 Elbow.reconfigure :loose @13:41:50.16 Forearm.seek CC @13:41:51.16 Hand.close @13:41:51.82 CC.seek :open @13:41:56.85 Forearm.seek :retract @13:41:57.83 Elbow.reconfigure :tight @13:41:57.88 Elbow.seek :garage @13:42:00.86 Elbow.reconfigure :loose @13:42:00.95 Forearm.reconfigure :tight @13:42:01.00 Forearm.seek :garage @13:42:01.97 Hand.open @13:42:02.62 Forearm.reconfigure :loose @13:42:02.67 Forearm.seek :retract @13:42:03.60 da sampling at most 1000ml, wcr at most 1000ml @13:42:03.64 Loading sh1 puck from tube 4 into Collection Clamp @13:42:03.65 DAfans.email "Started da in tube 4 at 13:42:03UTC27-Nov-23\n{1 => 33,\n 2 => 33,\n 3 => 2,\n 4 => 31,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@13:37:45, 31% flow, 16.3C, 27% humidity, 15.1psia, 13.715V, 0.254A, 91.589Ah, 3.49W",Subject: "da sampling at most 1000ml, wcr at most 1000ml" @13:42:03.93
SC.reconfigure :tight @13:42:03.98 SC.seek 4 @13:42:04.05 SC.configure :limp @13:42:04.31 Elbow.reconfigure :tight @13:42:04.35 Elbow.seek 4 @13:42:06.34 Forearm.reconfigure :tight @13:42:06.39 Forearm.seek 4 + 450 counts @13:42:07.46 SE.reconfigure :pick @13:42:07.54 SE.seek :beamBreak @13:42:08.04 upsync @13:42:08.67
SE.configure :default @13:42:08.84 Elbow.seek 4 @13:42:09.03 Forearm.seek 4 + 300 counts @13:42:09.25 Forearm.reconfigure :loose @13:42:09.30 Hand.close @13:42:09.95 SE.seek :down @13:42:10.54
Forearm.seek :retract @13:42:10.70 SE.stop @13:42:11.54
Elbow.seek CC @13:42:13.17 Elbow.reconfigure :loose @13:42:13.27 Forearm.reconfigure :tight @13:42:13.31 Forearm.seek CC @13:42:14.57 CC.seek :closed @13:42:19.08 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.30 @13:42:19.09 Hand.open @13:42:19.75 Forearm.reconfigure :loose @13:42:19.79 Forearm.seek :retract @13:42:20.77 collectSample 1000ml w/bubble point of 24psi @13:42:20.78 Priming 7ml @13:42:20.79 Intake.select :close @13:42:20.80 Exhaust.select :close @13:42:20.81 SS.hold @13:42:21.02 Calibrating pressure and temperature sensors @13:42:21.05 CTV.dial :air @13:42:21.12 SSV.dial :spit,avoiding: :exhaust @13:42:21.31 SS.reconfigure :fast @13:42:21.36 SS.seek 5ml @13:42:24.14 SSV.dial CBV @13:42:24.16
CBV.dial SSV @13:42:25.53 delay 3.8 seconds @13:42:29.46 Sensor limits: Intake max 104.8psia; Outlet max 96.9psia @13:42:29.47 SSV.dial :spit @13:42:30.81 SS.seek 0ml @13:42:33.63 SSV.dial CBV @13:42:34.95 CTV.dial :intake @13:42:35.75 SSV.dial CBV @13:42:35.77
CBV.dial SSV @13:42:36.01 Allowing 40s for 7.0ml of remaining 7.0ml @13:42:36.02 Intake.select :open @13:42:38.19 Intake @14.1psia, vacuum from 8.6 to 11.6psi @13:42:38.20 SS.reconfigure :inhaling 2.20ml/s @13:42:38.25 SS.seek 7ml @13:42:44.86 Stroke completed -- waiting for pressure to equalize ... @13:42:44.88 SS.reconfigure :exhale @13:42:44.92 SS.hold @13:42:50.80 Intake.select :close @13:42:50.95 SSV.dial :exhaust @13:42:51.71 Exhaust.select :open @13:42:51.82 SS.seek 0ml @13:42:56.07 Exhaust.select :close @13:42:56.18 Sampling 1000.0ml @13:42:56.19 Intake.select :close @13:42:56.20 Exhaust.select :close @13:42:56.22 SS.reconfigure :default @13:42:56.26 SS.hold @13:42:56.46 CTV.dial :intake @13:42:56.70 SSV.dial CBV @13:42:56.72
CBV.dial SSV @13:42:57.54 Allowing 130s for 25.0ml of remaining 1000.0ml @13:42:57.55 Intake.select :open @13:42:59.72 Intake @14.8psia, vacuum from 9.3 to 12.3psi @13:42:59.73 SS.reconfigure :inhaling 2.20ml/s @13:42:59.78 SS.seek 25ml @13:43:08.49 Vacuum too high -- waiting up to 40 seconds @13:43:08.50 SS.reconfigure :exhale @13:43:08.55 SS.hold @13:43:15.78 Intake @16.9psia, vacuum from 10.0 to 13.0psi @13:43:15.87 SS.reconfigure :inhaling 1.76ml/s @13:43:15.92 SS.seek 25ml @13:43:19.98 Vacuum too high -- waiting up to 40 seconds @13:43:20.00 SS.reconfigure :exhale @13:43:20.05 SS.hold @13:43:23.85 Intake @17.6psia, vacuum from 10.0 to 13.0psi @13:43:23.86 SS.reconfigure :inhaling 1.41ml/s @13:43:23.91 SS.seek 25ml @13:43:27.99 Vacuum too high -- waiting up to 40 seconds @13:43:28.00 SS.reconfigure :exhale @13:43:28.05 SS.hold @13:43:30.20 Intake @16.9psia, vacuum from 10.0 to 13.0psi @13:43:30.22 SS.reconfigure :inhaling 1.13ml/s @13:43:30.27 SS.seek 25ml @13:43:32.10 Stroke completed -- waiting for pressure to equalize ... @13:43:32.11 SS.reconfigure :exhale @13:43:32.16 SS.hold @13:43:52.49 Intake.select :close @13:43:52.64 SSV.dial :exhaust @13:43:53.40 Exhaust.select :open @13:43:53.51 SS.seek 0ml @13:44:02.32 Exhaust.select :close @13:44:02.43 SSV.dial CBV @13:44:02.45
CBV.dial SSV @13:44:03.22 Allowing 130s for 25.0ml of remaining 975.0ml @13:44:03.24 Intake.select :open @13:44:05.40 Intake @15.5psia, vacuum from 10.0 to 13.0psi @13:44:05.42 SS.reconfigure :inhaling 1.13ml/s @13:44:05.47 SS.seek 25ml @13:44:30.46 Stroke completed -- waiting for pressure to equalize ... @13:44:30.47 SS.reconfigure :exhale @13:44:30.52 SS.hold @13:44:57.44 Intake.select :close @13:44:57.59 SSV.dial :exhaust @13:44:58.36 Exhaust.select :open @13:44:58.47 SS.seek 0ml @13:45:07.25 Exhaust.select :close @13:45:07.36 SSV.dial CBV @13:45:07.39
CBV.dial SSV @13:45:08.16 Allowing 130s for 25.0ml of remaining 950.0ml @13:45:08.17 Intake.select :open @13:45:10.34 Intake @15.5psia, vacuum from 10.0 to 13.0psi @13:45:10.36 SS.reconfigure :inhaling 1.13ml/s @13:45:10.40 SS.seek 25ml @13:45:35.44 Stroke completed -- waiting for pressure to equalize ... @13:45:35.45 SS.reconfigure :exhale @13:45:35.50 SS.hold @13:46:01.92 Intake.select :close @13:46:02.08 SSV.dial :exhaust @13:46:02.85 Exhaust.select :open @13:46:02.97 SS.seek 0ml @13:46:11.74 Exhaust.select :close @13:46:11.84 SSV.dial CBV @13:46:11.86
CBV.dial SSV @13:46:12.64 Allowing 130s for 25.0ml of remaining 925.0ml @13:46:12.65 Intake.select :open @13:46:14.82 Intake @15.5psia, vacuum from 10.0 to 13.0psi @13:46:14.83 SS.reconfigure :inhaling 1.13ml/s @13:46:14.88 SS.seek 25ml @13:46:39.66 Stroke completed -- waiting for pressure to equalize ... @13:46:39.67 SS.reconfigure :exhale @13:46:39.71 SS.hold @13:47:04.10 Intake.select :close @13:47:04.25 SSV.dial :exhaust @13:47:05.00 Exhaust.select :open @13:47:05.12 SS.seek 0ml @13:47:13.91 Exhaust.select :close @13:47:14.01 SSV.dial CBV @13:47:14.04
CBV.dial SSV @13:47:14.81 Allowing 130s for 25.0ml of remaining 900.0ml @13:47:14.83 Intake.select :open @13:47:16.99 Intake @15.8psia, vacuum from 10.0 to 13.0psi @13:47:17.01 SS.reconfigure :inhaling 1.13ml/s @13:47:17.05 SS.seek 25ml @13:47:41.98 Stroke completed -- waiting for pressure to equalize ... @13:47:41.99 SS.reconfigure :exhale @13:47:42.04 SS.hold @13:48:04.48 Intake.select :close @13:48:04.64 SSV.dial :exhaust @13:48:05.41 Exhaust.select :open @13:48:05.52 SS.seek 0ml @13:48:14.31 Exhaust.select :close @13:48:14.42 SSV.dial CBV @13:48:14.44
CBV.dial SSV @13:48:15.22 Allowing 130s for 25.0ml of remaining 875.0ml @13:48:15.23 Intake.select :open @13:48:17.40 Intake @15.7psia, vacuum from 10.0 to 13.0psi @13:48:17.41 SS.reconfigure :inhaling 1.13ml/s @13:48:17.46 SS.seek 25ml @13:48:42.45 Stroke completed -- waiting for pressure to equalize ... @13:48:42.46 SS.reconfigure :exhale @13:48:42.51 SS.hold @13:49:04.85 Intake.select :close @13:49:05.01 SSV.dial :exhaust @13:49:05.77 Exhaust.select :open @13:49:05.88 SS.seek 0ml @13:49:14.62 Exhaust.select :close @13:49:14.73 SSV.dial CBV @13:49:14.76
CBV.dial SSV @13:49:15.53 Allowing 130s for 25.0ml of remaining 850.0ml @13:49:15.54 Intake.select :open @13:49:17.71 Intake @15.8psia, vacuum from 10.0 to 13.0psi @13:49:17.72 SS.reconfigure :inhaling 1.13ml/s @13:49:17.77 SS.seek 25ml @13:49:42.53 Stroke completed -- waiting for pressure to equalize ... @13:49:42.54 SS.reconfigure :exhale @13:49:42.59 SS.hold @13:50:05.35 Intake.select :close @13:50:05.51 SSV.dial :exhaust @13:50:06.28 Exhaust.select :open @13:50:06.40 SS.seek 0ml @13:50:15.15 Exhaust.select :close @13:50:15.26 SSV.dial CBV @13:50:15.30
CBV.dial SSV @13:50:16.05 Allowing 130s for 25.0ml of remaining 825.0ml @13:50:16.06 Intake.select :open @13:50:18.22 Intake @15.6psia, vacuum from 10.0 to 13.0psi @13:50:18.23 SS.reconfigure :inhaling 1.13ml/s @13:50:18.28 SS.seek 25ml @13:50:43.37 Stroke completed -- waiting for pressure to equalize ... @13:50:43.38 SS.reconfigure :exhale @13:50:43.43 SS.hold @13:51:05.92 Intake.select :close @13:51:06.08 SSV.dial :exhaust @13:51:06.86 Exhaust.select :open @13:51:07.49 SS.seek 0ml @13:51:16.26 Exhaust.select :close @13:51:16.37 SSV.dial CBV @13:51:16.39
CBV.dial SSV @13:51:17.19 Allowing 130s for 25.0ml of remaining 800.0ml @13:51:17.20 Intake.select :open @13:51:19.36 Intake @15.5psia, vacuum from 10.0 to 13.0psi @13:51:19.38 SS.reconfigure :inhaling 1.13ml/s @13:51:19.42 SS.seek 25ml @13:51:44.28 Stroke completed -- waiting for pressure to equalize ... @13:51:44.29 SS.reconfigure :exhale @13:51:44.33 SS.hold @13:52:06.77 Intake.select :close @13:52:06.92 SSV.dial :exhaust @13:52:07.68 Exhaust.select :open @13:52:07.80 SS.seek 0ml @13:52:16.75 Exhaust.select :close @13:52:16.86 SSV.dial CBV @13:52:16.88
CBV.dial SSV @13:52:17.74 Allowing 130s for 25.0ml of remaining 775.0ml @13:52:17.75 Intake.select :open @13:52:19.92 Intake @15.7psia, vacuum from 10.0 to 13.0psi @13:52:19.94 SS.reconfigure :inhaling 1.13ml/s @13:52:19.98 SS.seek 25ml @13:52:44.57 Stroke completed -- waiting for pressure to equalize ... @13:52:44.58 SS.reconfigure :exhale @13:52:44.63 SS.hold @13:53:07.07 Intake.select :close @13:53:07.22 SSV.dial :exhaust @13:53:08.00 Exhaust.select :open @13:53:08.16 SS.seek 0ml @13:53:16.95 Exhaust.select :close @13:53:17.05 SSV.dial CBV @13:53:17.08
CBV.dial SSV @13:53:17.85 Allowing 130s for 25.0ml of remaining 750.0ml @13:53:17.87 Intake.select :open @13:53:20.03 Intake @15.8psia, vacuum from 10.0 to 13.0psi @13:53:20.04 SS.reconfigure :inhaling 1.13ml/s @13:53:20.09 SS.seek 25ml @13:53:39.00 Vacuum too high -- waiting up to 40 seconds @13:53:39.01 SS.reconfigure :exhale @13:53:39.49 SS.hold @13:53:46.09 Intake @14.6psia, vacuum from 9.1 to 12.1psi @13:53:46.11 SS.reconfigure :inhaling 0.91ml/s @13:53:46.15 SS.seek 25ml @13:53:53.59 Stroke completed -- waiting for pressure to equalize ... @13:53:53.60 SS.reconfigure :exhale @13:53:53.64 SS.hold @13:54:11.98 Intake.select :close @13:54:12.13 SSV.dial :exhaust @13:54:12.88 Exhaust.select :open @13:54:13.00 SS.seek 0ml @13:54:22.02 Exhaust.select :close @13:54:22.13 SSV.dial CBV @13:54:22.15
CBV.dial SSV @13:54:22.95 Allowing 130s for 25.0ml of remaining 725.0ml @13:54:22.96 Intake.select :open @13:54:25.13 Intake @15.6psia, vacuum from 10.0 to 13.0psi @13:54:25.15 SS.reconfigure :inhaling 0.91ml/s @13:54:25.19 SS.seek 25ml @13:54:54.33 Stroke completed -- waiting for pressure to equalize ... @13:54:54.35 SS.reconfigure :exhale @13:54:54.39 SS.hold @13:55:16.83 Intake.select :close @13:55:16.98 SSV.dial :exhaust @13:55:17.76 Exhaust.select :open @13:55:17.87 SS.seek 0ml @13:55:26.54 Exhaust.select :close @13:55:26.65 SSV.dial CBV @13:55:26.67
CBV.dial SSV @13:55:27.46 Allowing 130s for 25.0ml of remaining 700.0ml @13:55:27.47 Intake.select :open @13:55:29.64 Intake @15.8psia, vacuum from 10.0 to 13.0psi @13:55:29.65 SS.reconfigure :inhaling 0.91ml/s @13:55:29.70 SS.seek 25ml @13:55:59.57 Stroke completed -- waiting for pressure to equalize ... @13:55:59.58 SS.reconfigure :exhale @13:55:59.62 SS.hold @13:56:21.96 Intake.select :close @13:56:22.11 SSV.dial :exhaust @13:56:23.30 Exhaust.select :open @13:56:23.42 SS.seek 0ml @13:56:32.19 Exhaust.select :close @13:56:32.30 SSV.dial CBV @13:56:32.34
CBV.dial SSV @13:56:33.08 Allowing 130s for 25.0ml of remaining 675.0ml @13:56:33.10 Intake.select :open @13:56:35.26 Intake @15.6psia, vacuum from 10.0 to 13.0psi @13:56:35.27 SS.reconfigure :inhaling 0.91ml/s @13:56:35.32 SS.seek 25ml @13:57:05.58 Stroke completed -- waiting for pressure to equalize ... @13:57:05.59 SS.reconfigure :exhale @13:57:05.64 SS.hold @13:57:30.12 Intake.select :close @13:57:30.27 SSV.dial :exhaust @13:57:31.04 Exhaust.select :open @13:57:31.16 SS.seek 0ml @13:57:40.01 Exhaust.select :close @13:57:40.13 SSV.dial CBV @13:57:40.17
CBV.dial SSV @13:57:40.91 Allowing 130s for 25.0ml of remaining 650.0ml @13:57:40.92 Intake.select :open @13:57:43.09 Intake @15.6psia, vacuum from 10.0 to 13.0psi @13:57:43.11 SS.reconfigure :inhaling 0.91ml/s @13:57:43.16 SS.seek 25ml @13:58:09.03 Vacuum too high -- waiting up to 40 seconds @13:58:09.04 SS.reconfigure :exhale @13:58:09.09 SS.hold @13:58:21.87 Intake @15.8psia, vacuum from 10.0 to 13.0psi @13:58:21.89 SS.reconfigure :inhaling 0.73ml/s @13:58:21.93 SS.seek 25ml @13:58:27.38 Stroke completed -- waiting for pressure to equalize ... @13:58:27.39 SS.reconfigure :exhale @13:58:27.43 SS.hold @13:58:41.80 Intake.select :close @13:58:41.95 SSV.dial :exhaust @13:58:42.71 Exhaust.select :open @13:58:42.82 SS.seek 0ml @13:58:51.77 Exhaust.select :close @13:58:51.88 SSV.dial CBV @13:58:51.90
CBV.dial SSV @13:58:52.68 Allowing 130s for 25.0ml of remaining 625.0ml @13:58:52.69 Intake.select :open @13:58:54.85 Intake @15.6psia, vacuum from 10.0 to 13.0psi @13:58:54.86 SS.reconfigure :inhaling 0.73ml/s @13:58:54.91 SS.seek 25ml @13:59:30.94 Stroke completed -- waiting for pressure to equalize ... @13:59:30.95 SS.reconfigure :exhale @13:59:30.99 SS.hold @13:59:55.45 Intake.select :close @13:59:55.60 SSV.dial :exhaust @13:59:56.38 Exhaust.select :open @13:59:56.49 SS.seek 0ml @14:00:05.19 Exhaust.select :close @14:00:05.31 SSV.dial CBV @14:00:05.35
CBV.dial SSV @14:00:06.10 Allowing 130s for 25.0ml of remaining 600.0ml @14:00:06.11 Intake.select :open @14:00:08.27 Intake @15.6psia, vacuum from 10.0 to 13.0psi @14:00:08.28 SS.reconfigure :inhaling 0.73ml/s @14:00:08.33 SS.seek 25ml @14:00:44.67 Stroke completed -- waiting for pressure to equalize ... @14:00:44.68 SS.reconfigure :exhale @14:00:44.73 SS.hold @14:01:11.17 Intake.select :close @14:01:11.33 SSV.dial :exhaust @14:01:12.10 Exhaust.select :open @14:01:12.22 SS.seek 0ml @14:01:20.95 Exhaust.select :close @14:01:21.06 SSV.dial CBV @14:01:21.08
CBV.dial SSV @14:01:21.89 Allowing 130s for 25.0ml of remaining 575.0ml @14:01:21.90 Intake.select :open @14:01:24.07 Intake @15.6psia, vacuum from 10.0 to 13.0psi @14:01:24.09 SS.reconfigure :inhaling 0.73ml/s @14:01:24.13 SS.seek 25ml @14:01:57.90 Vacuum too high -- waiting up to 40 seconds @14:01:57.91 SS.reconfigure :exhale @14:01:57.96 SS.hold @14:02:11.22 Intake @16.1psia, vacuum from 10.0 to 13.0psi @14:02:11.23 SS.reconfigure :inhaling 0.59ml/s @14:02:11.28 SS.seek 25ml @14:02:14.78 Stroke completed -- waiting for pressure to equalize ... @14:02:14.79 SS.reconfigure :exhale @14:02:14.84 SS.hold @14:02:29.71 Intake.select :close @14:02:29.86 SSV.dial :exhaust @14:02:30.63 Exhaust.select :open @14:02:30.74 SS.seek 0ml @14:02:39.47 Exhaust.select :close @14:02:39.58 SSV.dial CBV @14:02:39.60
CBV.dial SSV @14:02:40.37 Allowing 130s for 25.0ml of remaining 550.0ml @14:02:40.39 Intake.select :open @14:02:42.55 Intake @15.7psia, vacuum from 10.0 to 13.0psi @14:02:42.57 SS.reconfigure :inhaling 0.59ml/s @14:02:42.62 SS.seek 25ml @14:03:27.47 Stroke completed -- waiting for pressure to equalize ... @14:03:27.48 SS.reconfigure :exhale @14:03:27.52 SS.hold @14:03:52.45 Intake.select :close @14:03:52.60 SSV.dial :exhaust @14:03:53.39 Exhaust.select :open @14:03:53.50 SS.seek 0ml @14:04:02.22 Exhaust.select :close @14:04:02.37 SSV.dial CBV @14:04:02.39
CBV.dial SSV @14:04:03.19 Allowing 130s for 25.0ml of remaining 525.0ml @14:04:03.20 Intake.select :open @14:04:05.36 Intake @15.7psia, vacuum from 10.0 to 13.0psi @14:04:05.38 SS.reconfigure :inhaling 0.59ml/s @14:04:05.42 SS.seek 25ml @14:04:50.34 Stroke completed -- waiting for pressure to equalize ... @14:04:50.35 SS.reconfigure :exhale @14:04:50.40 SS.hold @14:05:19.22 Intake.select :close @14:05:19.37 SSV.dial :exhaust @14:05:20.14 Exhaust.select :open @14:05:20.25 SS.seek 0ml @14:05:29.06 Exhaust.select :close @14:05:29.17 SSV.dial CBV @14:05:29.19
CBV.dial SSV @14:05:29.98 Allowing 130s for 25.0ml of remaining 500.0ml @14:05:29.99 Intake.select :open @14:05:32.16 Intake @15.7psia, vacuum from 10.0 to 13.0psi @14:05:32.18 SS.reconfigure :inhaling 0.59ml/s @14:05:32.23 SS.seek 25ml @14:06:14.86 Vacuum too high -- waiting up to 40 seconds @14:06:14.88 SS.reconfigure :exhale @14:06:14.93 SS.hold @14:06:31.22 Intake @17.5psia, vacuum from 10.0 to 13.0psi @14:06:31.24 SS.reconfigure :inhaling 0.47ml/s @14:06:31.28 SS.seek 25ml @14:06:35.07 Stroke completed -- waiting for pressure to equalize ... @14:06:35.08 SS.reconfigure :exhale @14:06:35.13 SS.hold @14:06:51.76 Intake.select :close @14:06:51.91 SSV.dial :exhaust @14:06:52.67 Exhaust.select :open @14:06:52.78 SS.seek 0ml @14:07:01.78 Exhaust.select :close @14:07:01.88 SSV.dial CBV @14:07:01.91
CBV.dial SSV @14:07:02.68 Allowing 130s for 25.0ml of remaining 475.0ml @14:07:02.69 Intake.select :open @14:07:04.86 Intake @15.5psia, vacuum from 10.0 to 13.0psi @14:07:04.88 SS.reconfigure :inhaling 0.47ml/s @14:07:04.93 SS.seek 25ml @14:08:00.12 Stroke completed -- waiting for pressure to equalize ... @14:08:00.13 SS.reconfigure :exhale @14:08:00.17 SS.hold @14:08:28.75 Intake.select :close @14:08:28.90 SSV.dial :exhaust @14:08:29.68 Exhaust.select :open @14:08:29.80 SS.seek 0ml @14:08:38.79 Exhaust.select :close @14:08:38.90 SSV.dial CBV @14:08:38.92
CBV.dial SSV @14:08:39.71 Allowing 130s for 25.0ml of remaining 450.0ml @14:08:39.72 Intake.select :open @14:08:41.89 Intake @15.6psia, vacuum from 10.0 to 13.0psi @14:08:41.90 SS.reconfigure :inhaling 0.47ml/s @14:08:41.95 SS.seek 25ml @14:09:37.46 Stroke completed -- waiting for pressure to equalize ... @14:09:37.47 SS.reconfigure :exhale @14:09:37.52 SS.hold @14:10:10.26 Intake.select :close @14:10:10.41 SSV.dial :exhaust @14:10:11.17 Exhaust.select :open @14:10:11.29 SS.seek 0ml @14:10:20.10 Exhaust.select :close @14:10:20.21 SSV.dial CBV @14:10:20.23
CBV.dial SSV @14:10:21.02 Allowing 130s for 25.0ml of remaining 425.0ml @14:10:21.03 Intake.select :open @14:10:23.20 Intake @15.7psia, vacuum from 10.0 to 13.0psi @14:10:23.21 SS.reconfigure :inhaling 0.47ml/s @14:10:23.26 SS.seek 25ml @14:11:19.04 Stroke completed -- waiting for pressure to equalize ... @14:11:19.05 SS.reconfigure :exhale @14:11:19.09 SS.hold @14:11:51.76 Intake.select :close @14:11:51.91 SSV.dial :exhaust @14:11:52.70 Exhaust.select :open @14:11:52.82 SS.seek 0ml @14:12:01.86 Exhaust.select :close @14:12:01.97 SSV.dial CBV @14:12:02.00
CBV.dial SSV @14:12:02.79 Allowing 130s for 25.0ml of remaining 400.0ml @14:12:02.80 Intake.select :open @14:12:04.96 Intake @15.7psia, vacuum from 10.0 to 13.0psi @14:12:04.98 SS.reconfigure :inhaling 0.47ml/s @14:12:05.03 SS.seek 25ml @14:13:00.61 Stroke completed -- waiting for pressure to equalize ... @14:13:00.62 SS.reconfigure :exhale @14:13:00.67 SS.hold @14:13:39.65 Intake.select :close @14:13:39.81 SSV.dial :exhaust @14:13:40.58 Exhaust.select :open @14:13:40.69 SS.seek 0ml @14:13:49.47 Exhaust.select :close @14:13:49.58 SSV.dial CBV @14:13:49.61
CBV.dial SSV @14:13:50.39 Allowing 130s for 25.0ml of remaining 375.0ml @14:13:50.40 Intake.select :open @14:13:52.92 Intake @15.7psia, vacuum from 10.0 to 13.0psi @14:13:53.01 SS.reconfigure :inhaling 0.47ml/s @14:13:53.06 SS.seek 25ml @14:14:48.91 Stroke completed -- waiting for pressure to equalize ... @14:14:48.92 SS.reconfigure :exhale @14:14:48.97 SS.hold @14:15:29.14 Intake.select :close @14:15:29.30 SSV.dial :exhaust @14:15:30.06 Exhaust.select :open @14:15:30.18 SS.seek 0ml @14:15:38.97 Exhaust.select :close @14:15:39.08 SSV.dial CBV @14:15:39.10
CBV.dial SSV @14:15:39.89 Allowing 130s for 25.0ml of remaining 350.0ml @14:15:39.90 Intake.select :open @14:15:42.07 Intake @15.8psia, vacuum from 10.0 to 13.0psi @14:15:42.09 SS.reconfigure :inhaling 0.47ml/s @14:15:42.14 SS.seek 25ml @14:16:33.48 Vacuum too high -- waiting up to 40 seconds @14:16:33.49 SS.reconfigure :exhale @14:16:33.54 SS.hold @14:16:56.41 Intake @17.0psia, vacuum from 10.0 to 13.0psi @14:16:56.43 SS.reconfigure :inhaling 0.38ml/s @14:16:56.48 SS.seek 25ml @14:17:02.22 Stroke completed -- waiting for pressure to equalize ... @14:17:02.23 SS.reconfigure :exhale @14:17:02.28 SS.hold @14:17:28.80 Intake.select :close @14:17:28.96 SSV.dial :exhaust @14:17:29.74 Exhaust.select :open @14:17:29.86 SS.seek 0ml @14:17:38.64 Exhaust.select :close @14:17:38.75 SSV.dial CBV @14:17:38.77
CBV.dial SSV @14:17:39.65 Allowing 130s for 25.0ml of remaining 325.0ml @14:17:39.66 Intake.select :open @14:17:41.82 Intake @15.7psia, vacuum from 10.0 to 13.0psi @14:17:41.84 SS.reconfigure :inhaling 0.38ml/s @14:17:41.89 SS.seek 25ml @14:18:51.21 Stroke completed -- waiting for pressure to equalize ... @14:18:51.23 SS.reconfigure :exhale @14:18:51.27 SS.hold @14:19:31.45 Intake.select :close @14:19:31.61 SSV.dial :exhaust @14:19:32.37 Exhaust.select :open @14:19:32.49 SS.seek 0ml @14:19:41.34 Exhaust.select :close @14:19:41.45 SSV.dial CBV @14:19:41.47
CBV.dial SSV @14:19:42.28 Allowing 130s for 25.0ml of remaining 300.0ml @14:19:42.29 Intake.select :open @14:19:44.46 Intake @15.8psia, vacuum from 10.0 to 13.0psi @14:19:44.48 SS.reconfigure :inhaling 0.38ml/s @14:19:44.52 SS.seek 25ml @14:20:53.42 Stroke completed -- waiting for pressure to equalize ... @14:20:53.43 SS.reconfigure :exhale @14:20:53.47 SS.hold @14:21:33.65 SS.reconfigure :default @14:21:33.70 SS.hold @14:21:33.76 Intake.select :close @14:21:33.92 ShallowSampler::Clogged in MAIN -- Intake @16psia & Outlet @14psia won't equalize after 725.0ml @14:21:33.93 SSV.dial :exhaust @14:21:34.70 Exhaust.select :open @14:21:34.81 SS.reconfigure :exhale @14:21:34.85 SS.seek 0ml @14:21:43.98 Exhaust.select :close @14:21:44.09 Sampled 725.0ml @14:21:44.10 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @14:21:44.11 Intake.select :close @14:21:44.18 CSV.dial :waste @14:21:44.34 SSV.dial CBV,avoiding: :exhaust @14:21:44.37 CS.seek 0ml @14:21:44.38
CBV.dial SSV @14:21:44.54 CSV.dial :air @14:21:45.28 CS.seek 10ml @14:21:46.31
CTV.dial :air @14:21:47.06 delay 15 seconds @14:22:02.08 SSV.dial :spit,avoiding: :exhaust @14:22:03.41 CTV.dial CSV,avoiding: :intake @14:22:03.44
SS.seek 0ml @14:22:03.56 SSV.dial CBV,avoiding: :exhaust @14:22:09.88 SSV.dial between CBV and plug @14:22:10.37 CBV.dial between SSV and waste2 @14:22:10.78 CSV.dial between air and CTV @14:22:11.11 CS.seek 8.337ml @14:22:15.74 CTV.dial CSV,avoiding: :air @14:22:15.77
CSV.dial CTV @14:22:16.15 CS.reconfigure :pressurize @14:22:16.20 CS.seek 0ml @14:22:30.36 BubblePoint: Intake @24.1psia, Outlet @0.8psia @14:22:30.37 CBV.dial SSV @14:22:30.72 SS.reconfigure :default @14:22:30.76 SS.seek 10ml @14:22:36.21 delay 15 seconds @14:22:51.35 Evacuated: Intake @23.8psia, Outlet @23.6psia @14:22:51.36 SSV.dial :spit,avoiding: :exhaust @14:22:52.39 SS.reconfigure :exhale @14:22:52.44 SS.seek 0ml @14:22:57.41 SS.reconfigure :fast @14:22:57.46 SS.seek 5ml @14:23:00.40 SSV.dial CBV,avoiding: :exhaust @14:23:00.43
CBV.dial SSV @14:23:01.81 CTV.dial :air,avoiding: :intake @14:23:03.13 CSV.dial CTV @14:23:03.19
SS.seek 10ml @14:23:04.34 CSV.dial :air @14:23:04.79 CS.reconfigure :fast @14:23:04.84 CS.seek 0ml @14:23:05.82
SSV.dial :spit,avoiding: :exhaust @14:23:07.16 SS.reconfigure :exhale @14:23:07.20 SS.seek 0ml @14:23:12.22 SS.reconfigure :fast @14:23:12.27 SS.seek 5ml @14:23:12.91 CS.stop @14:23:15.09 SSV.dial CBV,avoiding: :exhaust @14:23:15.11
CBV.dial SSV @14:23:17.50 SSV.dial :spit,avoiding: :exhaust @14:23:18.83 SS.reconfigure :default @14:23:18.87 SS.seek 0ml @14:23:22.12 SS.stop @14:23:22.16 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @14:23:22.17 Intake.select :close @14:23:22.23 CSV.dial :waste @14:23:22.37 SSV.dial CBV,avoiding: :exhaust @14:23:22.39
CBV.dial SSV @14:23:22.98 CS.seek 0ml @14:23:23.04 CSV.dial :air @14:23:23.73
CTV.dial :air @14:23:23.80 CS.seek 10ml @14:23:23.98
delay 15 seconds @14:23:39.00 SSV.dial :spit,avoiding: :exhaust @14:23:40.34 CTV.dial CSV,avoiding: :intake @14:23:40.37
SS.reconfigure :exhale @14:23:40.47 SS.seek 0ml @14:23:40.84 SSV.dial CBV,avoiding: :exhaust @14:23:48.32 SSV.dial between CBV and plug @14:23:49.17 CBV.dial between SSV and waste2 @14:23:49.64 CSV.dial between air and CTV @14:23:49.95 CS.seek 7.475ml @14:23:56.51 CTV.dial CSV,avoiding: :air @14:23:56.53
CSV.dial CTV @14:23:56.93 CS.reconfigure :pressurize @14:23:56.98 CS.seek 0ml @14:24:12.33 BubblePoint: Intake @24.2psia, Outlet @3.3psia @14:24:12.34 CBV.dial SSV @14:24:12.68 SS.reconfigure :default @14:24:12.73 SS.seek 10ml @14:24:18.38 delay 15 seconds @14:24:33.51 Evacuated: Intake @23.9psia, Outlet @23.6psia @14:24:33.52 SSV.dial :spit,avoiding: :exhaust @14:24:34.57 SS.reconfigure :exhale @14:24:34.62 SS.seek 0ml @14:24:39.58 SS.reconfigure :fast @14:24:39.63 SS.seek 5ml @14:24:42.44 SSV.dial CBV,avoiding: :exhaust @14:24:42.45
CBV.dial SSV @14:24:43.82 CTV.dial :air,avoiding: :intake @14:24:45.13 CSV.dial CTV @14:24:45.17
SS.seek 10ml @14:24:46.35 CSV.dial :air @14:24:46.81 CS.reconfigure :fast @14:24:46.85 CS.seek 0ml @14:24:47.65
SSV.dial :spit,avoiding: :exhaust @14:24:48.98 SS.reconfigure :exhale @14:24:49.03 SS.seek 0ml @14:24:51.50 CS.stop @14:24:54.03
SS.reconfigure :fast @14:24:54.08 SS.seek 5ml @14:24:57.01 SSV.dial CBV,avoiding: :exhaust @14:24:57.03
CBV.dial SSV @14:24:59.42 SSV.dial :spit,avoiding: :exhaust @14:25:00.76 SS.reconfigure :default @14:25:00.81 SS.seek 0ml @14:25:03.94 SS.stop @14:25:03.98 Lysing: Adding 2.0ml AqMeOH @14:25:03.99 CBV.dial between CSV and waste1,avoiding: :DBV @14:25:05.49 CTV.dial CSV @14:25:06.80 CSV.dial CSR @14:25:07.71 Pulling 0.999ml of CSR.AqMeOH @14:25:07.72 CSR.select :AqMeOH @14:25:07.83 CS.reconfigure :default @14:25:08.31 CS.seek 1ml @14:25:11.72 delay 5 seconds @14:25:16.74 CSR.select :air @14:25:16.91 CS.seek 6ml @14:25:30.63 delay 2 seconds @14:25:32.65 CSV.dial :waste2 @14:25:33.12 CS.reconfigure :fast @14:25:33.17 CS.seek 0ml @14:25:47.56 CSV.dial CSR @14:25:48.14 Pulling 2ml of CSR.AqMeOH @14:25:48.15 CSR.select :AqMeOH @14:25:48.26 CS.reconfigure :default @14:25:48.31 CS.seek 2ml @14:25:54.32 delay 5 seconds @14:25:59.33 CSR.select :air @14:25:59.50 CS.seek 5.001ml @14:26:08.19 delay 2 seconds @14:26:10.21 CSV.dial CTV @14:26:10.68 CS.seek 1ml @14:26:21.86 Before lysing Collection Puck Heater at 13.3C/31.5psia @14:26:21.88 Heating Puck.from 4, :sh1 in Collection Clamp to 70C for 10:00 @14:26:21.90 CH.seek 70C,8:00 @14:28:43.91 CH.hold 10:00,70C @14:28:44.11 Begun lysing Collection Puck Heater at 68.1C/38.8psia @14:38:44.12 Ended lysing Collection Puck Heater at 70C/38.2psia @14:38:44.14 CS.seek 0ml @14:38:47.81 CTV.dial between spare and air,avoiding: :intake @14:38:48.83 CSV.dial :air @14:38:49.29 CS.seek 5ml @14:39:02.89 CSV.dial CTV @14:39:03.35 delay 2 seconds @14:39:05.37 CSV.dial :air @14:39:05.82 delay 2 seconds @14:39:07.84 CSV.dial CTV @14:39:08.74 delay 2 seconds @14:39:10.76 CSV.dial :air @14:39:11.21 delay 2 seconds @14:39:13.23 CS.seek 0ml @14:39:26.88 Puck @85.5psia after final lysis pressurization @14:39:26.90 Recovering Lysate into Collection syringe @14:39:26.91 CSV.dial CBV @14:39:27.93 CBV.dial CSV @14:39:28.32 CS.seek 5ml @14:39:42.24 delay 5 seconds @14:39:47.26 CTV.dial :air @14:39:47.79 CS.seek 7ml @14:39:53.68 delay 5 seconds @14:39:58.70 CSV.dial :air2 @14:39:59.14 CS.seek 10ml @14:40:07.59 CSV.dial between air and CTV,avoiding: CBV @14:40:08.74 Lysing Complete @14:40:08.81 Storing sh1 puck in Collection Clamp to tube 3 @14:40:08.98 SC.reconfigure :tight @14:40:09.03 SC.seek 3 @14:40:12.61 SC.configure :limp @14:40:12.83 Elbow.reconfigure :tight @14:40:12.87 Elbow.seek 3 @14:40:14.67 Forearm.reconfigure :tight @14:40:14.71 Forearm.seek 3 + 450 counts @14:40:15.84 SE.reconfigure :pick @14:40:15.90 SE.seek :beamBreak @14:40:18.94 SE.configure :default @14:40:19.10 Elbow.reconfigure :limp @14:40:19.15 Forearm.reconfigure :loose @14:40:19.19 Forearm.seek 3 @14:40:19.53 Elbow.hold @14:40:19.60 Elbow.reconfigure :tight @14:40:19.70 SE.seek 1.19 Pucks below Top Plate @14:40:20.14 Forearm.force -20 @14:40:20.19 Forearm.seek 3 + 278 counts @14:40:20.85 Forearm.reconfigure :tight @14:40:20.96 Forearm.seek 3 + 450 counts @14:40:21.23 SE.seek 2.19 Pucks below Top Plate @14:40:21.74 Forearm.reconfigure :loose @14:40:21.79 Forearm.seek :retract @14:40:22.71 Elbow.seek CC @14:40:24.44 Elbow.reconfigure :loose @14:40:24.54 Forearm.seek CC @14:40:25.50 Hand.close @14:40:26.15 CC.seek :open @14:40:30.87 Forearm.seek :retract @14:40:31.88 Elbow.reconfigure :tight @14:40:31.93 Elbow.seek 3 @14:40:33.70 Elbow.reconfigure :limp @14:40:33.75 Forearm.seek 3 @14:40:34.74 Elbow.hold @14:40:34.80 Elbow.reconfigure :tight @14:40:34.85 Hand.open @14:40:35.63 Forearm.force -20 @14:40:35.69 Forearm.seek 3 + 317 counts @14:40:35.96 Forearm.reconfigure :tight @14:40:36.01 Forearm.seek 3 + 450 counts @14:40:36.26 SE.seek :down @14:40:37.39
Forearm.reconfigure :loose @14:40:37.44 Forearm.seek :retract @14:40:38.85 SE.stop @14:40:38.90
Tranferring DA @14:40:38.91 Beginning samplePassC @14:40:38.92 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:40:39.10 Elbow.seek FlushPuck::Garage @14:40:40.99 Elbow.reconfigure :loose @14:40:41.09 Forearm.seek :garage @14:40:42.05 Hand.close @14:40:42.76 Forearm.seek :retract @14:40:43.74 Elbow.reconfigure :tight @14:40:43.79 Elbow.seek CC @14:40:46.76 Elbow.reconfigure :loose @14:40:46.86 Forearm.reconfigure :tight @14:40:46.91 Forearm.seek CC @14:40:48.14 CC.seek :closed @14:40:52.65 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.34 @14:40:52.72 Hand.open @14:40:53.33 Forearm.reconfigure :loose @14:40:53.38 Forearm.seek :retract @14:40:54.33 PSV.dial PSR @14:40:55.85 Pulling 1.5ml of PSR.flush PSR.select :flush @14:40:55.97 PS.seek 1.5ml @14:41:12.07 delay 5 seconds @14:41:17.09 PSV.dial :waste1 @14:41:17.56 PS.seek 0ml @14:41:33.71 PSV.dial PSR @14:41:34.18 PSR.select :air @14:41:34.29 PS.seek 2.5ml @14:42:00.72 PRV.dial CBV,avoiding: :ab2 @14:42:00.75
CBV.dial PRV @14:42:02.49 PSV.dial :mixloop @14:42:03.54 CTV.dial :air,avoiding: :intake @14:42:03.71 PTV.dial :PRVmixing @14:42:05.05 PS.seek 0ml @14:42:31.34 PSV.dial :air @14:42:31.81 PS.seek 2.5ml @14:42:58.15 PSV.dial :mixloop @14:42:58.62 PS.seek 0ml @14:43:24.93 PSV.dial :air @14:43:25.40 PS.seek 2.5ml @14:43:51.93 PSV.dial :mixloop @14:43:52.41 PS.seek 0ml @14:44:18.93 Garaging FlushPuck in Collection Clamp @14:44:19.00 Elbow.reconfigure :tight @14:44:19.05 Elbow.seek CC @14:44:19.34 Elbow.reconfigure :loose @14:44:19.44 Forearm.seek CC @14:44:20.48 Hand.close @14:44:21.15 CC.seek :open @14:44:25.76 Forearm.seek :retract @14:44:26.80 Elbow.reconfigure :tight @14:44:26.85 Elbow.seek :garage @14:44:29.81 Elbow.reconfigure :loose @14:44:29.90 Forearm.reconfigure :tight @14:44:29.95 Forearm.seek :garage @14:44:31.04 Hand.open @14:44:31.70 Forearm.reconfigure :loose @14:44:31.75 Forearm.seek :retract @14:44:32.75 PSV.dial :mixloop @14:44:32.82 PTV.dial :PRVmixing @14:44:32.90 PRV.dial CBV @14:44:32.92
CBV.dial PRV @14:44:33.12 CTV.dial :air,avoiding: :intake @14:44:33.14 Loading lyfil puck from tube 4 into Collection Clamp @14:44:33.37 SC.reconfigure :tight @14:44:33.85 SC.seek 4 @14:44:37.43 SC.configure :limp @14:44:37.64 Elbow.reconfigure :tight @14:44:37.69 Elbow.seek 4 @14:44:39.71 Forearm.reconfigure :tight @14:44:39.76 Forearm.seek 4 + 450 counts @14:44:40.85 SE.reconfigure :pick @14:44:40.91 SE.seek :beamBreak @14:44:42.15 SE.configure :default @14:44:42.31 Elbow.seek 4 @14:44:42.51 Forearm.seek 4 + 300 counts @14:44:42.73 Forearm.reconfigure :loose @14:44:42.78 Hand.close @14:44:43.41 SE.seek :down @14:44:43.61
Forearm.seek :retract @14:44:44.23 SE.stop @14:44:44.61
Elbow.seek CC @14:44:46.24 Elbow.reconfigure :loose @14:44:46.34 Forearm.reconfigure :tight @14:44:46.39 Forearm.seek CC @14:44:47.59 CC.seek :closed @14:44:52.47 Collection Clamp onSpring/offSpring for 12/26 samples, on/off torque 2.06 @14:44:52.49 Hand.open @14:44:53.20 Forearm.reconfigure :loose @14:44:53.25 Forearm.seek :retract @14:44:54.20 CTV.dial CSV,avoiding: :intake @14:44:55.56 CSV.dial CTV @14:44:55.90 PS.seek 2.5ml @14:45:22.27 CS.seek 4.5ml @14:45:37.23 delay 8 seconds @14:45:45.26 PSV.dial :air @14:45:45.87 delay 2 seconds @14:45:47.88 PSV.dial :waste2 @14:45:48.65 PS.seek 0ml @14:46:15.02 CBV.dial between PRV and waste1 @14:46:15.34 CSV.dial :air @14:46:15.82 CS.seek 10ml @14:46:30.70 CSV.dial CTV @14:46:31.16 CS.seek 0ml @14:46:57.61 CBV.dial :waste1 @14:46:57.92 delay 5 seconds @14:47:02.94 CTV.dial :air,avoiding: :intake @14:47:04.35 Storing lyfil puck in Collection Clamp to tube 3 @14:47:04.53 SC.reconfigure :tight @14:47:04.57 SC.seek 3 @14:47:08.16 SC.configure :limp @14:47:08.37 Elbow.reconfigure :tight @14:47:08.42 Elbow.seek 3 @14:47:10.19 Forearm.reconfigure :tight @14:47:10.24 Forearm.seek 3 + 450 counts @14:47:11.28 SE.reconfigure :pick @14:47:11.33 SE.seek :beamBreak @14:47:14.40 SE.configure :default @14:47:14.56 Elbow.reconfigure :limp @14:47:15.03 Forearm.reconfigure :loose @14:47:15.10 Forearm.seek 3 @14:47:15.37 Elbow.hold @14:47:15.44 Elbow.reconfigure :tight @14:47:15.54 SE.seek 2.19 Pucks below Top Plate @14:47:15.97 Forearm.force -20 @14:47:16.01 Forearm.seek 3 + 316 counts @14:47:16.29 Forearm.reconfigure :tight @14:47:16.34 Forearm.seek 3 + 450 counts @14:47:16.53 SE.seek 3.19 Pucks below Top Plate @14:47:17.03 Forearm.reconfigure :loose @14:47:17.08 Forearm.seek :retract @14:47:18.02 Elbow.seek CC @14:47:19.70 Elbow.reconfigure :loose @14:47:19.80 Forearm.seek CC @14:47:20.79 Hand.close @14:47:21.39 CC.seek :open @14:47:26.12 Forearm.seek :retract @14:47:27.11 Elbow.reconfigure :tight @14:47:27.16 Elbow.seek 3 @14:47:28.93 Elbow.reconfigure :limp @14:47:28.98 Forearm.seek 3 @14:47:30.01 Elbow.hold @14:47:30.08 Elbow.reconfigure :tight @14:47:30.13 Hand.open @14:47:30.88 Forearm.force -20 @14:47:30.94 Forearm.seek 3 + 311 counts @14:47:31.66 Forearm.reconfigure :tight @14:47:31.71 Forearm.seek 3 + 450 counts @14:47:31.97 SE.seek :down @14:47:33.11
Forearm.reconfigure :loose @14:47:33.15 Forearm.seek :retract @14:47:34.46 SE.stop @14:47:34.50
PSV.dial :air @14:47:35.24 PS.seek 2.5ml @14:48:01.64 delay 1 second @14:48:02.66 PSV.dial :mixloop @14:48:03.11 delay 1 second @14:48:04.13 PSV.dial :air @14:48:04.60 delay 1 second @14:48:05.61 PSV.dial :mixloop @14:48:06.08 delay 1 second @14:48:07.10 PSV.dial :air @14:48:07.57 delay 1 second @14:48:08.59 PS.seek 0ml @14:48:35.14 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @14:48:35.32 Elbow.seek FlushPuck::Garage @14:48:37.13 Elbow.reconfigure :loose @14:48:37.23 Forearm.seek :garage @14:48:38.25 Hand.close @14:48:39.00 Forearm.seek :retract @14:48:40.00 Elbow.reconfigure :tight @14:48:40.05 Elbow.seek PC @14:48:41.30 Elbow.reconfigure :loose @14:48:41.40 Forearm.reconfigure :tight @14:48:41.45 Forearm.seek PC @14:48:42.65 PC.seek :closed @14:48:47.33 Processing Clamp onSpring/offSpring for 16/26 samples, on/off torque 1.63 @14:48:47.40 Hand.open @14:48:48.07 Forearm.reconfigure :loose @14:48:48.12 Forearm.seek :retract @14:48:49.12 PRV.dial :air @14:48:49.58 PSV.dial :mixloop @14:48:50.11 PS.seek 0.3ml @14:48:53.95 PSV.dial PSR @14:48:55.02 Pulling 0.4ml of PSR.air @14:48:55.03 PSR.select :air @14:48:55.05 PS.seek 0.7ml @14:49:00.02 delay 3 seconds @14:49:03.03 PSR.select :air @14:49:03.06 PS.seek 2.5ml @14:49:22.20 delay 3 seconds @14:49:25.22 PSV.dial :mixloop @14:49:26.28 PTV.dial :mixing @14:49:27.05 PBV.dial :waste2 @14:49:28.01 PS.seek 0ml @14:49:54.36 Garaging FlushPuck in Processing Clamp @14:49:54.43 Elbow.reconfigure :tight @14:49:54.48 Elbow.seek PC @14:49:54.60 Elbow.reconfigure :loose @14:49:54.70 Forearm.seek PC @14:49:55.67 Hand.close @14:49:56.26 PC.seek :open @14:50:00.98 Forearm.seek :retract @14:50:01.95 Elbow.reconfigure :tight @14:50:02.00 Elbow.seek :garage @14:50:03.36 Elbow.reconfigure :loose @14:50:03.46 Forearm.reconfigure :tight @14:50:03.51 Forearm.seek :garage @14:50:04.46 Hand.open @14:50:05.07 Forearm.reconfigure :loose @14:50:05.54 Forearm.seek :retract @14:50:06.57 Loading da puck from tube 4 into Processing Clamp @14:50:06.80 SC.reconfigure :tight @14:50:06.85 SC.seek 4 @14:50:10.46 SC.configure :limp @14:50:10.67 Elbow.reconfigure :tight @14:50:10.72 Elbow.seek 4 @14:50:12.55 Forearm.reconfigure :tight @14:50:12.61 Forearm.seek 4 + 450 counts @14:50:13.66 SE.reconfigure :pick @14:50:13.72 SE.seek :beamBreak @14:50:14.95 SE.configure :default @14:50:15.10 Elbow.seek 4 @14:50:15.29 Forearm.seek 4 + 300 counts @14:50:15.48 Forearm.reconfigure :loose @14:50:15.52 Hand.close @14:50:16.15 SE.seek :down @14:50:16.35
Forearm.seek :retract @14:50:17.02 SE.stop @14:50:17.29
Elbow.seek PC @14:50:19.85 Elbow.reconfigure :loose @14:50:19.95 Forearm.reconfigure :tight @14:50:19.99 Forearm.seek PC @14:50:21.20 PC.seek :closed @14:50:25.84 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.62 @14:50:25.85 Hand.open @14:50:26.57 Forearm.reconfigure :loose @14:50:26.62 Forearm.seek :retract @14:50:27.57 Heating Puck.from 4, :da in Processing Clamp to 28C for 2:00:00 @14:50:27.58 PH.seek 28C,5:00 @14:51:09.82 PH.hold 2:00:00,28C @14:51:09.87 delay 1:30 @14:52:39.92 PSR.select :air @14:52:39.93 PSV.dial PSR @14:52:41.00 Pulling 0.5ml of PSR.wash @14:52:41.01 PSR.select :wash @14:52:41.13 PS.seek 0.5ml @14:52:47.07 delay 5 seconds @14:52:52.47 PSR.select :air @14:52:52.66 PS.seek 2ml @14:53:08.82 PTV.dial :puck @14:53:09.58 PSV.dial PTV @14:53:10.34 PBV.dial :waste1 @14:53:11.75 PS.seek 1.5ml @14:53:17.67 delay 3 seconds @14:53:20.69 PBV.dial between PSV and waste1 @14:53:21.05 PS.seek 0ml @14:53:37.14 delay 5:00 @14:58:37.16 recoverPSRreagent08 PTV.dial :puck @14:58:37.17 PBV.dial :waste1 @14:58:37.55 delay 30 seconds @14:59:07.58 PBV.dial between PSV and waste1 @14:59:07.94 PSR.select :air @14:59:07.96 PSV.dial PSR @14:59:08.73 PS.reconfigure :fast @14:59:08.78 PS.seek 2.5ml @14:59:32.60 PSV.dial PTV @14:59:33.37 delay 5 seconds @14:59:38.39 PSV.dial PSR @14:59:39.13 delay 5 seconds @14:59:44.15 PSV.dial PTV @14:59:44.91 delay 5 seconds @14:59:49.93 PSV.dial :air @14:59:50.70 PS.seek 0ml @15:00:13.98 PTV.dial :PRVmixing,via: PRV @15:00:15.30 PSV.dial :mixloop @15:00:15.77 PRV.dial :ab1 @15:00:16.53 PS.reconfigure :slow @15:00:16.57 PS.seek 0.15ml @15:00:20.10 delay 2 seconds @15:00:22.12 CBV.dial PRV @15:00:22.14
PRV.dial CBV @15:00:22.71 PS.seek 0.35ml @15:00:27.35 delay 2 seconds @15:00:29.36 CBV.dial between PRV and PSV @15:00:29.69 PRV.dial :air @15:00:30.15 PS.seek 0.9ml @15:00:41.85 PRV.dial :waste2 @15:00:42.60 PS.seek 0.5ml @15:00:51.32 addPMLReagent08 @15:00:51.33 PSV.dial :air @15:00:51.79 PS.reconfigure :default @15:00:51.84 PS.seek 2ml @15:01:07.94 PSV.dial :PML @15:01:08.41 PTV.dial :mixing @15:01:09.18 PBV.dial :waste1 @15:01:10.04 PS.seek 1.85ml @15:01:12.35 PBV.dial between PSV and waste1 @15:01:12.73 PS.seek 0ml @15:01:32.44 delay 2 seconds @15:01:34.46 Beginning samplePassC @15:01:34.47 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @15:01:34.66 Elbow.reconfigure :tight @15:01:34.71 Elbow.seek FlushPuck::Garage @15:01:36.09 Elbow.reconfigure :loose @15:01:36.19 Forearm.seek :garage @15:01:37.20 Hand.close @15:01:38.00 Forearm.seek :retract @15:01:38.99 Elbow.reconfigure :tight @15:01:39.04 Elbow.seek CC @15:01:41.92 Elbow.reconfigure :loose @15:01:42.02 Forearm.reconfigure :tight @15:01:42.06 Forearm.seek CC @15:01:43.37 CC.seek :closed @15:01:47.91 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.48 @15:01:47.97 Hand.open @15:01:48.69 Forearm.reconfigure :loose @15:01:48.74 Forearm.seek :retract @15:01:49.70 PSV.dial PSR @15:01:50.89 Pulling 1.5ml of PSR.flush @15:01:50.90 PSR.select :flush @15:01:51.02 PS.seek 1.5ml @15:02:07.25 delay 5 seconds @15:02:12.27 PSV.dial :waste1 @15:02:13.21 PS.seek 0ml @15:02:29.41 PSV.dial PSR @15:02:29.88 PSR.select :air @15:02:30.00 PS.seek 2.5ml @15:02:56.42 PRV.dial CBV,avoiding: :ab2 @15:02:56.44
CBV.dial PRV @15:02:58.08 PSV.dial :mixloop @15:02:59.13 CTV.dial :air,avoiding: :intake @15:02:59.20 PTV.dial :PRVmixing @15:02:59.98 PS.seek 0ml @15:03:26.31 PSV.dial :air @15:03:26.79 PS.seek 2.5ml @15:03:53.12 PSV.dial :mixloop @15:03:53.60 PS.seek 0ml @15:04:19.94 PSV.dial :air @15:04:20.41 PS.seek 2.5ml @15:04:46.69 PSV.dial :mixloop @15:04:47.16 PS.seek 0ml @15:05:13.53 Garaging FlushPuck in Collection Clamp @15:05:13.60 Elbow.reconfigure :tight @15:05:13.65 Elbow.seek CC @15:05:13.96 Elbow.reconfigure :loose @15:05:14.11 Forearm.seek CC @15:05:15.15 Hand.close @15:05:15.75 CC.seek :open @15:05:20.37 Forearm.seek :retract @15:05:21.35 Elbow.reconfigure :tight @15:05:21.39 Elbow.seek :garage @15:05:24.34 Elbow.reconfigure :loose @15:05:24.44 Forearm.reconfigure :tight @15:05:24.49 Forearm.seek :garage @15:05:25.46 Hand.open @15:05:26.18 Forearm.reconfigure :loose @15:05:26.23 Forearm.seek :retract @15:05:27.21 delayUntil "15:09:34.45" @15:05:27.23 Whole Cell Archive sampling 1000ml @15:05:27.25 Loading wcr puck from tube 4 into Collection Clamp @15:05:28.17 Elbow.reconfigure :tight @15:05:28.22 Elbow.seek 4 @15:05:30.16 Forearm.reconfigure :tight @15:05:30.21 Forearm.seek 4 + 450 counts @15:05:31.34 SE.reconfigure :pick @15:05:31.40 SE.seek :beamBreak @15:05:32.75 SE.configure :default @15:05:32.91 Elbow.seek 4 @15:05:33.11 Forearm.seek 4 + 300 counts @15:05:33.40 Forearm.reconfigure :loose @15:05:33.45 Hand.close @15:05:34.14 SE.seek :down @15:05:34.34 Forearm.seek :retract @15:05:35.07 SE.stop @15:05:35.28 Elbow.seek CC @15:05:36.92 Elbow.reconfigure :loose @15:05:37.02 Forearm.reconfigure :tight @15:05:37.07 Forearm.seek CC @15:05:38.31 CC.seek :closed @15:05:42.82 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.35 @15:05:42.84 Hand.open @15:05:43.57 Forearm.reconfigure :loose @15:05:43.62 Forearm.seek :retract @15:05:44.61 collectSample 1000ml w/bubble point of 35psi @15:05:44.63 Priming 7ml @15:05:44.64 Intake.select :close @15:05:44.65 Exhaust.select :close @15:05:44.66 SS.hold @15:05:44.87 Calibrating pressure sensors @15:05:44.88 CTV.dial :air @15:05:44.95 SSV.dial :spit,avoiding: :exhaust @15:05:45.04 SS.reconfigure :fast @15:05:45.10 SS.seek 5ml @15:05:47.81 SSV.dial CBV @15:05:47.86 CBV.dial SSV @15:05:49.19 delay 3.8 seconds @15:05:53.13 Sensor limits: Intake max 105.7psia; Outlet max 96.8psia @15:05:53.14 SSV.dial :spit @15:05:54.48 SS.seek 0ml @15:05:57.61 SSV.dial CBV @15:05:58.95 CTV.dial :intake @15:05:59.70 SSV.dial CBV @15:05:59.72 CBV.dial SSV @15:05:59.81 Allowing 40s for 7.0ml of remaining 7.0ml @15:05:59.82 Intake.select :open @15:06:01.99 Intake @14.2psia, vacuum from 8.7 to 11.7psi @15:06:02.01 SS.reconfigure :inhaling 2.20ml/s @15:06:02.06 SS.seek 7ml @15:06:08.24 Stroke completed -- waiting for pressure to equalize ... @15:06:08.25 SS.reconfigure :exhale @15:06:08.30 SS.hold @15:06:24.77 Intake.select :close @15:06:24.93 SSV.dial :exhaust @15:06:25.71 Exhaust.select :open @15:06:25.83 SS.seek 0ml @15:06:29.90 Exhaust.select :close @15:06:30.00 Sampling 1000.0ml @15:06:30.02 Intake.select :close @15:06:30.03 Exhaust.select :close @15:06:30.04 SS.reconfigure :default @15:06:30.08 SS.hold @15:06:30.31 CTV.dial :intake @15:06:30.39 SSV.dial CBV @15:06:30.43 CBV.dial SSV @15:06:31.19 Allowing 130s for 25.0ml of remaining 1000.0ml @15:06:31.21 Intake.select :open @15:06:33.38 Intake @15.1psia, vacuum from 9.6 to 12.6psi @15:06:33.40 SS.reconfigure :inhaling 2.20ml/s @15:06:33.45 SS.seek 25ml @15:06:41.82 Vacuum too high -- waiting up to 40 seconds @15:06:41.84 SS.reconfigure :exhale @15:06:41.89 SS.hold @15:06:51.25 Intake @18.3psia, vacuum from 10.0 to 13.0psi @15:06:51.27 SS.reconfigure :inhaling 1.76ml/s @15:06:51.32 SS.seek 25ml @15:06:53.64 Vacuum too high -- waiting up to 40 seconds @15:06:53.65 SS.reconfigure :exhale @15:06:53.71 SS.hold @15:06:56.48 Intake @18.4psia, vacuum from 10.0 to 13.0psi @15:06:56.49 SS.reconfigure :inhaling 1.41ml/s @15:06:56.54 SS.seek 25ml @15:06:59.36 Vacuum too high -- waiting up to 40 seconds @15:06:59.37 SS.reconfigure :exhale @15:06:59.86 SS.hold @15:07:01.99 Intake @18.5psia, vacuum from 10.0 to 13.0psi @15:07:02.01 SS.reconfigure :inhaling 1.13ml/s @15:07:02.05 SS.seek 25ml @15:07:05.04 Vacuum too high -- waiting up to 40 seconds @15:07:05.06 SS.reconfigure :exhale @15:07:05.11 SS.hold @15:07:07.69 Intake @18.6psia, vacuum from 10.0 to 13.0psi @15:07:07.71 SS.reconfigure :inhaling 0.91ml/s @15:07:07.76 SS.seek 25ml @15:07:11.82 Vacuum too high -- waiting up to 40 seconds @15:07:11.84 SS.reconfigure :exhale @15:07:11.89 SS.hold @15:07:14.01 SS.reconfigure :inhaling 0.73ml/s @15:07:14.07 SS.seek 25ml @15:07:17.23 Stroke completed -- waiting for pressure to equalize ... @15:07:17.24 SS.reconfigure :exhale @15:07:17.29 SS.hold @15:07:46.27 Intake.select :close @15:07:46.50 SSV.dial :exhaust @15:07:47.29 Exhaust.select :open @15:07:47.40 SS.seek 0ml @15:07:56.49 Exhaust.select :close @15:07:56.60 SSV.dial CBV @15:07:56.62 CBV.dial SSV @15:07:57.41 Allowing 130s for 25.0ml of remaining 975.0ml @15:07:57.43 Intake.select :open @15:07:59.60 Intake @15.5psia, vacuum from 10.0 to 13.0psi @15:07:59.61 SS.reconfigure :inhaling 0.73ml/s @15:07:59.66 SS.seek 25ml @15:08:35.48 Stroke completed -- waiting for pressure to equalize ... @15:08:35.50 SS.reconfigure :exhale @15:08:35.55 SS.hold @15:09:06.49 Intake.select :close @15:09:06.65 SSV.dial :exhaust @15:09:07.41 Exhaust.select :open @15:09:07.53 SS.seek 0ml @15:09:16.44 Exhaust.select :close @15:09:16.55 SSV.dial CBV @15:09:16.57 CBV.dial SSV @15:09:17.35 Allowing 130s for 25.0ml of remaining 950.0ml @15:09:17.36 Intake.select :open @15:09:19.53 Intake @15.6psia, vacuum from 10.0 to 13.0psi @15:09:19.55 SS.reconfigure :inhaling 0.73ml/s @15:09:19.60 SS.seek 25ml @15:09:34.46 recoverPRVreagent08 @15:09:34.47 PTV.dial :mixing @15:09:35.24 PBV.dial :waste1 @15:09:35.62 delay 30 seconds @15:09:55.87 Stroke completed -- waiting for pressure to equalize ... @15:09:55.89 SS.reconfigure :exhale @15:09:55.93 SS.hold @15:10:05.70 PBV.dial between PSV and waste1 @15:10:06.09 PSV.dial :air @15:10:06.69 PS.reconfigure :fast @15:10:06.74 PS.seek 2.5ml @15:10:26.58 Intake.select :close @15:10:26.75 SSV.dial :exhaust @15:10:27.52 Exhaust.select :open @15:10:27.63 SS.seek 0ml @15:10:30.93 PSV.dial :mixloop @15:10:31.51 delay 2 seconds @15:10:33.53 PSV.dial :air @15:10:33.99 delay 2 seconds @15:10:36.01 PSV.dial :mixloop @15:10:36.49 delay 2 seconds @15:10:36.70 Exhaust.select :close @15:10:36.81 SSV.dial CBV @15:10:36.83 CBV.dial SSV @15:10:37.63 Allowing 130s for 25.0ml of remaining 925.0ml @15:10:38.02 Intake.select :open @15:10:38.51 PSV.dial :air @15:10:38.98 PS.seek 0ml @15:10:40.25 Intake @15.5psia, vacuum from 10.0 to 13.0psi @15:10:40.27 SS.reconfigure :inhaling 0.73ml/s @15:10:40.32 SS.seek 25ml @15:11:02.46 washCycleDA #1 of 2 @15:11:02.47 PSR.select :air @15:11:02.49 PSV.dial PSR @15:11:03.84 Pulling 0.5ml of PSR.wash @15:11:03.85 PSR.select :wash @15:11:03.97 PS.reconfigure :default @15:11:04.02 PS.seek 0.5ml @15:11:10.02 delay 5 seconds @15:11:15.04 PSR.select :air @15:11:15.16 PS.seek 2ml @15:11:16.65 Stroke completed -- waiting for pressure to equalize ... @15:11:16.67 SS.reconfigure :exhale @15:11:16.71 SS.hold @15:11:31.63 PTV.dial :puck @15:11:32.39 PSV.dial PTV @15:11:33.26 PBV.dial :waste1 @15:11:33.69 PS.seek 1.5ml @15:11:39.71 delay 3 seconds @15:11:41.26 Intake.select :close @15:11:41.42 SSV.dial :exhaust @15:11:42.19 Exhaust.select :open @15:11:42.31 SS.seek 0ml @15:11:42.73 PBV.dial between PSV and waste1 @15:11:43.11 PS.seek 0ml @15:11:51.12 Exhaust.select :close @15:11:51.23 SSV.dial CBV @15:11:51.26 CBV.dial SSV @15:11:52.03 Allowing 130s for 25.0ml of remaining 900.0ml @15:11:52.05 Intake.select :open @15:11:54.21 Intake @15.8psia, vacuum from 10.0 to 13.0psi @15:11:54.23 SS.reconfigure :inhaling 0.73ml/s @15:11:54.28 SS.seek 25ml @15:11:59.31 delay 2:00 @15:12:30.89 Stroke completed -- waiting for pressure to equalize ... @15:12:30.90 SS.reconfigure :exhale @15:12:30.95 SS.hold @15:12:55.89 Intake.select :close @15:12:56.05 SSV.dial :exhaust @15:12:56.81 Exhaust.select :open @15:12:56.93 SS.seek 0ml @15:13:05.97 Exhaust.select :close @15:13:06.08 SSV.dial CBV @15:13:06.10 CBV.dial SSV @15:13:06.89 Allowing 130s for 25.0ml of remaining 875.0ml @15:13:06.90 Intake.select :open @15:13:09.07 Intake @15.7psia, vacuum from 10.0 to 13.0psi @15:13:09.09 SS.reconfigure :inhaling 0.73ml/s @15:13:09.14 SS.seek 25ml @15:13:45.23 Stroke completed -- waiting for pressure to equalize ... @15:13:45.24 SS.reconfigure :exhale @15:13:45.29 SS.hold @15:13:59.32 recoverPSRreagent08 @15:13:59.34 PTV.dial :puck @15:13:59.52 PBV.dial :waste1 @15:13:59.91 delay 30 seconds @15:14:07.96 Intake.select :close @15:14:08.12 SSV.dial :exhaust @15:14:08.89 Exhaust.select :open @15:14:09.00 SS.seek 0ml @15:14:18.03 Exhaust.select :close @15:14:18.14 SSV.dial CBV @15:14:18.16 CBV.dial SSV @15:14:18.93 Allowing 130s for 25.0ml of remaining 850.0ml @15:14:18.95 Intake.select :open @15:14:21.11 Intake @15.7psia, vacuum from 10.0 to 13.0psi @15:14:21.13 SS.reconfigure :inhaling 0.73ml/s @15:14:21.18 SS.seek 25ml @15:14:29.95 PBV.dial between PSV and waste1 @15:14:30.32 PSR.select :air @15:14:30.34 PSV.dial PSR @15:14:31.10 PS.reconfigure :fast @15:14:31.16 PS.seek 2.5ml @15:14:55.36 PSV.dial PTV @15:14:56.15 delay 5 seconds @15:14:57.99 Stroke completed -- waiting for pressure to equalize ... @15:14:58.01 SS.reconfigure :exhale @15:14:58.05 SS.hold @15:15:01.16 PSV.dial PSR @15:15:01.93 delay 5 seconds @15:15:06.94 PSV.dial PTV @15:15:07.73 delay 5 seconds @15:15:12.74 PSV.dial :air @15:15:13.63 PS.seek 0ml @15:15:22.58 Intake.select :close @15:15:22.74 SSV.dial :exhaust @15:15:23.51 Exhaust.select :open @15:15:23.63 SS.seek 0ml @15:15:32.65 Exhaust.select :close @15:15:32.76 SSV.dial CBV @15:15:32.80 CBV.dial SSV @15:15:33.58 Allowing 130s for 25.0ml of remaining 825.0ml @15:15:33.59 Intake.select :open @15:15:35.76 Intake @15.4psia, vacuum from 9.9 to 12.9psi @15:15:35.78 SS.reconfigure :inhaling 0.73ml/s @15:15:35.83 SS.seek 25ml @15:15:37.21 washCycleDA #2 of 2 @15:15:37.22 PSR.select :air @15:15:37.24 PSV.dial PSR @15:15:38.60 Pulling 0.5ml of PSR.wash @15:15:38.61 PSR.select :wash @15:15:38.73 PS.reconfigure :default @15:15:38.77 PS.seek 0.5ml @15:15:44.64 delay 5 seconds @15:15:49.66 PSR.select :air @15:15:49.78 PS.seek 2ml @15:16:06.05 PTV.dial :puck @15:16:06.13 PSV.dial PTV @15:16:07.02 PBV.dial :waste1 @15:16:07.45 PS.seek 1.5ml @15:16:11.94 Stroke completed -- waiting for pressure to equalize ... @15:16:11.96 SS.reconfigure :exhale @15:16:12.00 SS.hold @15:16:13.45 delay 3 seconds @15:16:16.47 PBV.dial between PSV and waste1 @15:16:16.89 PS.seek 0ml @15:16:33.11 delay 2:00 @15:16:36.60 Intake.select :close @15:16:36.76 SSV.dial :exhaust @15:16:37.54 Exhaust.select :open @15:16:37.66 SS.seek 0ml @15:16:46.68 Exhaust.select :close @15:16:46.80 SSV.dial CBV @15:16:46.82 CBV.dial SSV @15:16:47.60 Allowing 130s for 25.0ml of remaining 800.0ml @15:16:47.62 Intake.select :open @15:16:49.79 Intake @15.5psia, vacuum from 10.0 to 13.0psi @15:16:49.81 SS.reconfigure :inhaling 0.73ml/s @15:16:49.86 SS.seek 25ml @15:17:25.99 Stroke completed -- waiting for pressure to equalize ... @15:17:26.00 SS.reconfigure :exhale @15:17:26.05 SS.hold @15:17:53.06 Intake.select :close @15:17:53.22 SSV.dial :exhaust @15:17:54.00 Exhaust.select :open @15:17:54.12 SS.seek 0ml @15:18:03.15 Exhaust.select :close @15:18:03.66 SSV.dial CBV @15:18:03.69 CBV.dial SSV @15:18:04.48 Allowing 130s for 25.0ml of remaining 775.0ml @15:18:04.49 Intake.select :open @15:18:06.66 Intake @15.5psia, vacuum from 10.0 to 13.0psi @15:18:06.68 SS.reconfigure :inhaling 0.73ml/s @15:18:06.73 SS.seek 25ml @15:18:33.14 recoverPSRreagent08 @15:18:33.15 PTV.dial :puck @15:18:33.23 PBV.dial :waste1 @15:18:33.61 delay 30 seconds @15:18:39.72 Vacuum too high -- waiting up to 40 seconds @15:18:39.74 SS.reconfigure :exhale @15:18:39.79 SS.hold @15:18:54.31 Intake @17.2psia, vacuum from 10.0 to 13.0psi @15:18:54.33 SS.reconfigure :inhaling 0.59ml/s @15:18:54.38 SS.seek 25ml @15:18:58.55 Stroke completed -- waiting for pressure to equalize ... @15:18:58.63 SS.reconfigure :exhale @15:18:58.68 SS.hold @15:19:03.63 PBV.dial between PSV and waste1 @15:19:03.98 PSR.select :air @15:19:04.00 PSV.dial PSR @15:19:04.75 PS.reconfigure :fast @15:19:04.80 PS.seek 2.5ml @15:19:13.06 Intake.select :close @15:19:13.22 SSV.dial :exhaust @15:19:13.97 Exhaust.select :open @15:19:14.09 SS.seek 0ml @15:19:22.93 Exhaust.select :close @15:19:23.04 SSV.dial CBV @15:19:23.06 CBV.dial SSV @15:19:23.83 Allowing 130s for 25.0ml of remaining 750.0ml @15:19:24.31 Intake.select :open @15:19:26.47 Intake @15.4psia, vacuum from 9.9 to 12.9psi @15:19:26.49 SS.reconfigure :inhaling 0.59ml/s @15:19:26.54 SS.seek 25ml @15:19:29.04 PSV.dial PTV @15:19:29.83 delay 5 seconds @15:19:34.85 PSV.dial PSR @15:19:35.63 delay 5 seconds @15:19:40.65 PSV.dial PTV @15:19:41.42 delay 5 seconds @15:19:46.44 PSV.dial :air @15:19:47.22 PS.seek 0ml @15:20:10.76 getPMLReagent08 @15:20:10.78 PRV.dial :air @15:20:11.22 delay 5 seconds @15:20:11.47 Stroke completed -- waiting for pressure to equalize ... @15:20:11.48 SS.reconfigure :exhale @15:20:11.53 SS.hold @15:20:16.23 PTV.dial :PRVmixing @15:20:17.58 delay 5 seconds @15:20:22.66 PRV.dial :ab2 @15:20:23.11 PTV.dial :PRVmixing @15:20:23.18 PSV.dial :mixloop @15:20:24.16 PS.reconfigure :default @15:20:24.23 PS.seek 0.3ml @15:20:28.14 delay 2 seconds @15:20:30.15 PRV.dial :air @15:20:30.68 PS.seek 0.55ml @15:20:34.26 addPMLReagent08 @15:20:34.27 PSV.dial :air @15:20:34.74 PS.seek 2ml @15:20:38.16 Intake.select :close @15:20:38.32 SSV.dial :exhaust @15:20:39.09 Exhaust.select :open @15:20:39.21 SS.seek 0ml @15:20:48.07 Exhaust.select :close @15:20:48.18 SSV.dial CBV @15:20:48.20 CBV.dial SSV @15:20:48.97 Allowing 130s for 25.0ml of remaining 725.0ml @15:20:48.99 Intake.select :open @15:20:50.45 PSV.dial :PML @15:20:50.93 PTV.dial :mixing @15:20:51.15 Intake @15.7psia, vacuum from 10.0 to 13.0psi @15:20:51.17 SS.reconfigure :inhaling 0.59ml/s @15:20:51.22 SS.seek 25ml @15:20:51.70 PBV.dial :waste1 @15:20:52.12 PS.seek 1.85ml @15:20:54.48 PBV.dial between PSV and waste1 @15:20:54.85 PS.seek 0ml @15:21:14.51 delay 2 seconds @15:21:16.53 delay 8:00 @15:21:36.11 Stroke completed -- waiting for pressure to equalize ... @15:21:36.13 SS.reconfigure :exhale @15:21:36.17 SS.hold @15:22:05.23 Intake.select :close @15:22:05.39 SSV.dial :exhaust @15:22:06.18 Exhaust.select :open @15:22:06.30 SS.seek 0ml @15:22:15.13 Exhaust.select :close @15:22:15.65 SSV.dial CBV @15:22:15.67 CBV.dial SSV @15:22:16.46 Allowing 130s for 25.0ml of remaining 700.0ml @15:22:16.47 Intake.select :open @15:22:18.64 Intake @15.7psia, vacuum from 10.0 to 13.0psi @15:22:18.66 SS.reconfigure :inhaling 0.59ml/s @15:22:18.71 SS.seek 25ml @15:22:59.18 Vacuum too high -- waiting up to 40 seconds @15:22:59.20 SS.reconfigure :exhale @15:22:59.25 SS.hold @15:23:13.64 Intake @16.3psia, vacuum from 10.0 to 13.0psi @15:23:13.66 SS.reconfigure :inhaling 0.47ml/s @15:23:13.71 SS.seek 25ml @15:23:19.25 Stroke completed -- waiting for pressure to equalize ... @15:23:19.26 SS.reconfigure :exhale @15:23:19.31 SS.hold @15:23:34.24 Intake.select :close @15:23:34.40 SSV.dial :exhaust @15:23:35.17 Exhaust.select :open @15:23:35.29 SS.seek 0ml @15:23:44.28 Exhaust.select :close @15:23:44.39 SSV.dial CBV @15:23:44.44 CBV.dial SSV @15:23:45.19 Allowing 130s for 25.0ml of remaining 675.0ml @15:23:45.20 Intake.select :open @15:23:47.37 Intake @15.6psia, vacuum from 10.0 to 13.0psi @15:23:47.39 SS.reconfigure :inhaling 0.47ml/s @15:23:47.44 SS.seek 25ml @15:24:42.67 Stroke completed -- waiting for pressure to equalize ... @15:24:42.68 SS.reconfigure :exhale @15:24:42.73 SS.hold @15:25:11.63 Intake.select :close @15:25:11.79 SSV.dial :exhaust @15:25:13.02 Exhaust.select :open @15:25:13.14 SS.seek 0ml @15:25:21.95 Exhaust.select :close @15:25:22.06 SSV.dial CBV @15:25:22.08 CBV.dial SSV @15:25:22.88 Allowing 130s for 25.0ml of remaining 650.0ml @15:25:22.89 Intake.select :open @15:25:25.06 Intake @15.4psia, vacuum from 9.9 to 12.9psi @15:25:25.08 SS.reconfigure :inhaling 0.47ml/s @15:25:25.13 SS.seek 25ml @15:26:20.64 Stroke completed -- waiting for pressure to equalize ... @15:26:20.65 SS.reconfigure :exhale @15:26:20.70 SS.hold @15:26:51.81 Intake.select :close @15:26:51.97 SSV.dial :exhaust @15:26:52.73 Exhaust.select :open @15:26:52.85 SS.seek 0ml @15:27:01.92 Exhaust.select :close @15:27:02.03 SSV.dial CBV @15:27:02.05 CBV.dial SSV @15:27:02.83 Allowing 130s for 25.0ml of remaining 625.0ml @15:27:02.84 Intake.select :open @15:27:05.01 Intake @15.4psia, vacuum from 9.9 to 12.9psi @15:27:05.03 SS.reconfigure :inhaling 0.47ml/s @15:27:05.08 SS.seek 25ml @15:28:00.46 Stroke completed -- waiting for pressure to equalize ... @15:28:00.47 SS.reconfigure :exhale @15:28:00.52 SS.hold @15:28:37.30 Intake.select :close @15:28:37.46 SSV.dial :exhaust @15:28:38.23 Exhaust.select :open @15:28:38.35 SS.seek 0ml @15:28:47.21 Exhaust.select :close @15:28:47.32 SSV.dial CBV @15:28:47.35 CBV.dial SSV @15:28:48.14 Allowing 130s for 25.0ml of remaining 600.0ml @15:28:48.15 Intake.select :open @15:28:50.32 Intake @15.4psia, vacuum from 9.9 to 12.9psi @15:28:50.34 SS.reconfigure :inhaling 0.47ml/s @15:28:50.39 SS.seek 25ml @15:29:16.55 recoverPRVreagent08 @15:29:16.56 PTV.dial :mixing @15:29:16.63 PBV.dial :waste1 @15:29:17.00 delay 30 seconds @15:29:45.93 Stroke completed -- waiting for pressure to equalize ... @15:29:45.94 SS.reconfigure :exhale @15:29:46.39 SS.hold @15:29:47.02 PBV.dial between PSV and waste1 @15:29:47.39 PSV.dial :air @15:29:47.96 PS.reconfigure :fast @15:29:48.01 PS.seek 2.5ml @15:30:12.25 PSV.dial :mixloop @15:30:13.14 delay 2 seconds @15:30:15.22 PSV.dial :air @15:30:15.81 delay 2 seconds @15:30:17.83 PSV.dial :mixloop @15:30:18.29 delay 2 seconds @15:30:20.30 PSV.dial :air @15:30:20.77 PS.seek 0ml @15:30:26.64 SS.reconfigure :default @15:30:26.70 SS.hold @15:30:26.76 Intake.select :close @15:30:26.92 ShallowSampler::Clogged in WCR -- Intake @16psia & Outlet @14psia won't equalize after 425.0ml @15:30:26.93 SSV.dial :exhaust @15:30:27.70 Exhaust.select :open @15:30:27.81 SS.reconfigure :exhale @15:30:27.86 SS.seek 0ml @15:30:36.76 Exhaust.select :close @15:30:36.87 Sampled 425.0ml @15:30:36.89 Drying puck before fixing for 5:00 @15:30:36.90 CBV.dial :waste2b @15:30:37.66 delay 1:00 @15:30:44.47 washCycleDA #1 of 3 @15:30:44.48 PSR.select :air @15:30:44.50 PSV.dial PSR @15:30:45.85 Pulling 0.5ml of PSR.wash @15:30:45.86 PSR.select :wash @15:30:45.97 PS.reconfigure :default @15:30:46.02 PS.seek 0.5ml @15:30:52.00 delay 5 seconds @15:30:57.02 PSR.select :air @15:30:57.14 PS.seek 2ml @15:31:13.28 PTV.dial :puck @15:31:14.03 PSV.dial PTV @15:31:14.91 PBV.dial :waste1 @15:31:15.34 PS.seek 1.5ml @15:31:21.34 delay 3 seconds @15:31:24.36 PBV.dial between PSV and waste1 @15:31:24.74 PS.seek 0ml @15:31:37.69 CSV.dial :air @15:31:38.15 CS.reconfigure :fast @15:31:38.21 CS.seek 10ml @15:31:41.56 delay 2:00 @15:32:02.76 Pumping wcr puck from tube 4 up to 35psi... @15:32:02.77 CBV.dial SSV @15:32:04.04 CBV.dial between CSV and waste2b @15:32:05.02 CSV.dial between air and CTV @15:32:05.34 CS.seek 7.349ml @15:32:12.17 CTV.dial CSV,avoiding: :air @15:32:12.19 CSV.dial CTV @15:32:13.04 CS.reconfigure :pressurize @15:32:13.09 CS.seek 0ml @15:32:26.99 CBV.dial :waste2b @15:32:27.34 CS.reconfigure :default @15:32:27.39 CS.seek 0ml @15:32:33.88 delay 1:00 @15:33:33.91 CTV.dial :air,avoiding: :intake @15:33:35.24 Fix #1 of 2 (with RNAlater) for 2:30 @15:33:35.26 CSV.dial CSR @15:33:35.70 Pulling 2ml of CSR.RNAlater @15:33:35.72 CSR.select :RNAlater @15:33:35.83 CS.seek 2ml @15:33:41.58 recoverPSRreagent08 @15:33:41.59 PTV.dial :puck @15:33:41.78 PBV.dial :waste1 @15:33:41.85 delay 5 seconds @15:33:42.16 delay 30 seconds @15:33:46.87 CSR.select :air @15:33:46.99 CS.reconfigure :fast @15:33:47.04 CS.seek 10ml @15:34:06.85 Pumping wcr puck from tube 4 up to 35psi... @15:34:06.87 CBV.dial SSV @15:34:08.12 CBV.dial between CSV and waste2b @15:34:09.09 CSV.dial between air and CTV @15:34:09.70 CS.seek 7.265ml @15:34:12.18 PBV.dial between PSV and waste1 @15:34:12.55 PSR.select :air @15:34:12.56 PSV.dial PSR @15:34:13.32 PS.reconfigure :fast @15:34:13.37 PS.seek 2.5ml @15:34:16.79 CTV.dial CSV,avoiding: :air @15:34:16.81 CSV.dial CTV @15:34:18.64 CS.reconfigure :pressurize @15:34:18.72 CS.seek 0ml @15:34:37.12 delay 2:30 @15:34:37.56 PSV.dial PTV @15:34:38.45 delay 5 seconds @15:34:43.47 PSV.dial PSR @15:34:44.22 delay 5 seconds @15:34:49.24 PSV.dial PTV @15:34:50.00 delay 5 seconds @15:34:55.02 PSV.dial :air @15:34:55.79 PS.seek 0ml @15:35:19.25 washCycleDA #2 of 3 @15:35:19.26 PSR.select :air @15:35:19.27 PSV.dial PSR @15:35:20.62 Pulling 0.5ml of PSR.wash @15:35:20.63 PSR.select :wash @15:35:20.75 PS.reconfigure :default @15:35:20.80 PS.seek 0.5ml @15:35:26.81 delay 5 seconds @15:35:31.83 PSR.select :air @15:35:31.95 PS.seek 2ml @15:35:48.06 PTV.dial :puck @15:35:48.14 PSV.dial PTV @15:35:48.89 PBV.dial :waste1 @15:35:49.32 PS.seek 1.5ml @15:35:55.22 delay 3 seconds @15:35:58.23 PBV.dial between PSV and waste1 @15:35:58.61 PS.seek 0ml @15:36:14.72 delay 2:00 @15:37:07.14 CBV.dial :waste2b @15:37:07.49 CS.reconfigure :default @15:37:07.54 CS.seek 0ml @15:37:08.94 delay 1:00 @15:38:08.96 CTV.dial :air,avoiding: :intake @15:38:10.29 Fix #2 of 2 (with RNAlater) for 2:30 @15:38:10.30 CSV.dial CSR @15:38:10.73 Pulling 2ml of CSR.RNAlater @15:38:10.74 CSR.select :RNAlater @15:38:10.86 CS.seek 2ml @15:38:14.74 recoverPSRreagent08 @15:38:14.75 PTV.dial :puck @15:38:14.82 PBV.dial :waste1 @15:38:15.20 delay 30 seconds @15:38:17.03 delay 5 seconds @15:38:22.05 CSR.select :air @15:38:22.16 CS.reconfigure :fast @15:38:22.21 CS.seek 10ml @15:38:42.00 Pumping wcr puck from tube 4 up to 35psi... @15:38:42.02 CBV.dial SSV @15:38:43.28 CBV.dial between CSV and waste2b @15:38:44.26 CSV.dial between air and CTV @15:38:44.99 CS.seek 7.203ml @15:38:45.50 PBV.dial between PSV and waste1 @15:38:45.88 PSR.select :air @15:38:45.90 PSV.dial PSR @15:38:46.67 PS.reconfigure :fast @15:38:46.72 PS.seek 2.5ml @15:38:52.19 CTV.dial CSV,avoiding: :air @15:38:52.22 CSV.dial CTV @15:38:53.63 CS.reconfigure :pressurize @15:38:53.68 CS.seek 0ml @15:39:08.92 delay 2:30 @15:39:10.74 PSV.dial PTV @15:39:11.51 delay 5 seconds @15:39:16.53 PSV.dial PSR @15:39:17.29 delay 5 seconds @15:39:22.31 PSV.dial PTV @15:39:23.09 delay 5 seconds @15:39:28.11 PSV.dial :air @15:39:28.87 PS.seek 0ml @15:39:52.37 washCycleDA #3 of 3 @15:39:52.38 PSR.select :air @15:39:52.40 PSV.dial PSR @15:39:53.74 Pulling 0.5ml of PSR.wash @15:39:53.75 PSR.select :wash @15:39:53.87 PS.reconfigure :default @15:39:53.92 PS.seek 0.5ml @15:39:59.80 delay 5 seconds @15:40:04.82 PSR.select :air @15:40:04.94 PS.seek 2ml @15:40:21.08 PTV.dial :puck @15:40:21.16 PSV.dial PTV @15:40:22.04 PBV.dial :waste1 @15:40:22.47 PS.seek 1.5ml @15:40:28.40 delay 3 seconds @15:40:31.42 PBV.dial between PSV and waste1 @15:40:31.79 PS.seek 0ml @15:40:47.93 delay 2:00 @15:41:38.94 CBV.dial :waste2b @15:41:39.30 CS.reconfigure :default @15:41:39.35 CS.seek 0ml @15:41:43.81 delay 1:00 @15:42:43.84 CTV.dial :air,avoiding: :intake @15:42:47.95 recoverPSRreagent08 @15:42:47.96 PTV.dial :puck @15:42:48.03 PBV.dial :waste1 @15:42:48.41 delay 30 seconds @15:43:18.44 PBV.dial between PSV and waste1 @15:43:18.80 PSR.select :air @15:43:18.82 PSV.dial PSR @15:43:19.59 PS.reconfigure :fast @15:43:19.64 PS.seek 2.5ml @15:43:43.92 PSV.dial PTV @15:43:44.71 delay 5 seconds @15:43:49.73 PSV.dial PSR @15:43:50.52 delay 5 seconds @15:43:55.54 PSV.dial PTV @15:43:56.43 delay 5 seconds @15:44:01.45 PSV.dial :air @15:44:02.71 PS.seek 0ml @15:44:26.11 Gate.power :camera,:ON @15:44:26.13 Mixing DA Substrates PSR.select :air @15:44:26.15 PSV.dial PSR @15:44:27.58 Pulling 0.25ml of PSR.sub1 @15:44:27.61 PSR.select :sub1 @15:44:27.79 PS.reconfigure :default @15:44:27.92 PS.seek 0.25ml @15:44:31.41 delay 5 seconds @15:44:36.43 Pulling 0.25ml of PSR.sub2 @15:44:36.44 PSR.select :sub2 @15:44:36.56 PS.seek 0.5ml @15:44:39.92 delay 5 seconds @15:44:44.94 PSR.select :air @15:44:45.06 PS.seek 2ml @15:45:01.30 PTV.dial :puck @15:45:01.38 PSV.dial PTV @15:45:02.16 PBV.dial :waste1 @15:45:02.59 PS.seek 1.5ml @15:45:08.52 delay 3 seconds @15:45:11.54 PBV.dial between PSV and waste1 @15:45:11.92 PS.seek 0ml @15:45:28.30 delay 10 seconds @15:45:38.33 recoverPSRreagent08 PTV.dial :puck @15:45:38.41 PBV.dial :waste1 @15:45:38.78 delay 30 seconds @15:46:08.81 PBV.dial between PSV and waste1 @15:46:09.19 PSR.select :air @15:46:09.21 PSV.dial PSR @15:46:09.99 PS.reconfigure :fast @15:46:10.04 PS.seek 2.5ml @15:46:34.45 PSV.dial PTV @15:46:35.24 delay 5 seconds @15:46:40.26 PSV.dial PSR @15:46:41.03 delay 5 seconds @15:46:46.05 PSV.dial PTV @15:46:46.83 delay 5 seconds @15:46:51.85 PSV.dial :air @15:46:52.63 PS.seek 0ml @15:47:16.80 Additional air clear @15:47:16.81 PSR.select :air @15:47:16.83 PSV.dial PSR @15:47:18.20 Pulling 2ml of PSR.air @15:47:18.21 PSR.select :air @15:47:18.24 PS.reconfigure :default @15:47:18.28 PS.seek 2ml @15:47:39.64 delay 5 seconds @15:47:44.66 PTV.dial :puck @15:47:44.73 PSV.dial PTV @15:47:45.50 PBV.dial :waste1 @15:47:45.93 PS.seek 1.5ml @15:47:51.93 delay 3 seconds @15:47:54.95 PBV.dial between PSV and waste1 @15:47:55.31 delay 1 second @15:47:56.33 PSV.dial :waste1 @15:47:57.42 PS.seek 0ml @15:48:13.63 Processing Puck Heater at 27.8C before imaging @15:48:13.64 PH.coast @15:48:13.74 Imaging da puck in Processing Clamp while storing it to tube 3 @15:48:14.34 SC.reconfigure :tight @15:48:14.45 SC.seek 3 @15:48:18.05 SC.configure :limp @15:48:18.27 Elbow.reconfigure :tight @15:48:18.32 Elbow.seek 3 @15:48:20.08 Forearm.reconfigure :tight @15:48:20.13 Forearm.seek 3 + 450 counts @15:48:21.17 SE.reconfigure :pick @15:48:21.23 SE.seek :beamBreak @15:48:24.18 SE.configure :default @15:48:24.34 Elbow.reconfigure :limp @15:48:24.39 Forearm.reconfigure :loose @15:48:24.44 Forearm.seek 3 @15:48:24.64 Elbow.hold @15:48:24.70 Elbow.reconfigure :tight @15:48:24.80 SE.seek 3.17 Pucks below Top Plate @15:48:25.25 Forearm.force -20 @15:48:25.29 Forearm.seek 3 + 310 counts @15:48:25.58 Forearm.reconfigure :tight @15:48:25.63 Forearm.seek 3 + 450 counts @15:48:25.83 SE.seek 4.17 Pucks below Top Plate @15:48:26.35 Forearm.reconfigure :loose @15:48:26.39 Forearm.seek :retract @15:48:27.33 Elbow.seek PC @15:48:29.82 Elbow.reconfigure :loose @15:48:29.92 Forearm.seek PC @15:48:30.85 Hand.close @15:48:31.59 PC.seek :open @15:48:36.38 Forearm.seek :retract @15:48:37.39 Elbow.reconfigure :tight @15:48:37.44 Elbow.seek 3 @15:48:40.56 Forearm.reconfigure :tight @15:48:40.61 Forearm.seek 3 + 450 counts @15:48:41.55 Hand.open @15:48:42.27 SE.seek 3.82 Pucks below Top Plate @15:48:42.54 SE.reconfigure :seal @15:48:42.59 SE.seek 2.86 Pucks below Top Plate @15:48:43.15 Lights, camera, ACTION! @15:48:43.19 PuckCamera: snap -bin=2,2 -auto /var/log/esp/da23nov2715h725ml.tif @15:49:02.65 Waiting >20 seconds for da to relinquish FlushPuck @15:49:06.55 SX HX9/16 (USB:05): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 20.061 seconds /var/log/esp/da23nov2715h725ml.tif: TIFF Upload Complete @15:49:06.58 PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da23nov2715h725ml15s.tif @15:49:22.23 SX HX9/16 (USB:05): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15 seconds /var/log/esp/midres/da23nov2715h725ml15s.tif: TIFF Upload Complete @15:49:22.26 PuckCamera: snap -bin=2,2 20.0 /var/log/esp/midres/da23nov2715h725ml20s.tif @15:49:42.87 SX HX9/16 (USB:05): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 20 seconds /var/log/esp/midres/da23nov2715h725ml20s.tif: TIFF Upload Complete @15:49:42.89 Gate.power :camera,:OFF @15:49:42.91 DAfans.email "20.061 sec auto-exposure",Subject: "da23nov2715h725ml images!" @15:49:43.44 Elbow.seek 3 @15:49:43.67 Forearm.seek 3 + 450 counts @15:49:43.77 SE.reconfigure :gently @15:49:43.82 SE.seek 3.57 Pucks below Top Plate @15:49:44.52 Elbow.reconfigure :limp @15:49:44.58 Forearm.reconfigure :loose @15:49:44.65 Forearm.seek 3 @15:49:44.92 Elbow.hold @15:49:44.99 Elbow.reconfigure :tight @15:49:45.05 SE.reconfigure :default @15:49:45.10 SE.seek 3.76 Pucks below Top Plate @15:49:45.50 Forearm.force -20 @15:49:45.54 Forearm.seek 3 + 299 counts @15:49:45.87 Forearm.reconfigure :tight @15:49:45.94 Forearm.seek 3 + 450 counts @15:49:46.35 SE.seek :down @15:49:47.62 Forearm.reconfigure :loose @15:49:47.72 Forearm.seek :retract @15:49:48.84 SE.stop @15:49:50.07 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @15:49:50.60 Elbow.seek FlushPuck::Garage @15:49:52.63 Elbow.reconfigure :loose @15:49:52.83 Forearm.seek :garage @15:49:53.82 Hand.close @15:49:54.72 Forearm.seek :retract @15:49:55.74 Elbow.reconfigure :tight @15:49:55.83 Elbow.seek PC @15:49:57.18 Elbow.reconfigure :loose @15:49:57.39 Forearm.reconfigure :tight @15:49:57.56 Forearm.seek PC @15:49:59.29 PC.seek :closed @15:50:04.12 Processing Clamp onSpring/offSpring for 15/25 samples, on/off torque 1.63 @15:50:04.25 Hand.open @15:50:04.98 Forearm.reconfigure :loose @15:50:05.09 Forearm.seek :retract @15:50:06.11 Storing wcr puck in Collection Clamp to tube 3 @15:50:06.15 PSR.select :air @15:50:06.23 PSV.dial PSR @15:50:06.90 Pulling 0.5ml of PSR.flush @15:50:06.97 PSR.select :flush @15:50:07.30 Elbow.reconfigure :tight @15:50:07.42 Elbow.seek 3 @15:50:07.46 PS.seek 0.5ml @15:50:10.35 Forearm.reconfigure :tight @15:50:10.45 Forearm.seek 3 + 450 counts @15:50:11.69 SE.reconfigure :pick @15:50:11.81 SE.seek :beamBreak @15:50:14.47 delay 5 seconds @15:50:14.79 SE.configure :default @15:50:15.11 Elbow.reconfigure :limp @15:50:15.26 Forearm.reconfigure :loose @15:50:15.39 Forearm.seek 3 @15:50:15.71 Elbow.hold @15:50:15.84 Elbow.reconfigure :tight @15:50:16.03 SE.seek 4.17 Pucks below Top Plate @15:50:16.70 Forearm.force -20 @15:50:16.80 Forearm.seek 3 + 318 counts @15:50:17.27 Forearm.reconfigure :tight @15:50:17.37 Forearm.seek 3 + 450 counts @15:50:17.63 SE.seek 5.17 Pucks below Top Plate @15:50:18.39 Forearm.reconfigure :loose @15:50:18.49 Forearm.seek :retract @15:50:19.49 Elbow.seek CC @15:50:19.56 PSR.select :air @15:50:19.68 PS.seek 2.5ml @15:50:19.86 upsync @15:50:21.12 Elbow.reconfigure :loose @15:50:21.22 Forearm.seek CC @15:50:22.17 Hand.close @15:50:22.84 CC.seek :open @15:50:28.04 Forearm.seek :retract @15:50:29.06 Elbow.reconfigure :tight @15:50:29.12 Elbow.seek 3 @15:50:30.90 Elbow.reconfigure :limp @15:50:30.95 Forearm.seek 3 @15:50:31.96 Elbow.hold @15:50:32.03 Elbow.reconfigure :tight @15:50:32.08 Hand.open @15:50:32.84 Forearm.force -20 @15:50:32.89 Forearm.seek 3 + 314 counts @15:50:33.18 Forearm.reconfigure :tight @15:50:33.23 Forearm.seek 3 + 450 counts @15:50:33.48 SE.seek :down @15:50:34.62 Forearm.reconfigure :loose @15:50:34.67 Forearm.seek :retract @15:50:35.83 SE.stop @15:50:35.88
Starting finalPurge @15:50:41.01 delay 1 second @15:50:42.03 PRV.dial :waste2,avoiding: :ab2 @15:50:43.90 PTV.dial :PRVmixing @15:50:45.23 PSV.dial :mixloop @15:50:46.28 PS.seek :empty @15:50:55.84
Waiting >20 seconds for psFlush to relinquish FlushPuck @15:51:12.60 PSR.select :air @15:51:12.62 PSV.dial PSR @15:51:13.68 Pulling 0.5ml of PSR.flush @15:51:13.69 PSR.select :flush @15:51:13.81 PS.seek 0.5ml @15:51:19.73 delay 5 seconds @15:51:24.75 PSR.select :air @15:51:24.87 PS.seek 2.5ml @15:51:46.13 delay 1 second @15:51:47.20 PBV.dial :waste1 @15:51:47.56 PTV.dial :mixing @15:51:48.32 PSV.dial :mixloop @15:51:49.39 PS.seek :empty @15:52:15.78 PSV.dial PSR @15:52:16.82 PSR.select :flush @15:52:16.95 PS.seek 1ml @15:52:28.04 PSR.select :air @15:52:28.15 PS.seek 2.5ml @15:52:44.38 PSV.dial PTV @15:52:45.15 PTV.dial :puck @15:52:45.90 PBV.dial :waste1 @15:52:45.92 PS.seek 0ml @15:53:12.44 PSV.dial PSR @15:53:13.20 PSR.select :flush @15:53:13.32 PS.seek 0.5ml @15:53:19.32 PSR.select :air @15:53:19.44 PS.seek 2.5ml @15:53:40.76 PSV.dial PTV @15:53:41.52 PBV.dial PSV @15:53:42.09 PS.seek 0ml @15:54:08.47 PSV.dial PBV @15:54:08.95 PS.seek 2.5ml @15:54:35.32 PSV.dial :waste1 @15:54:36.08 PS.seek 0ml @15:55:02.60 PSV.dial PSR @15:55:03.07 PSR.select :air @15:55:03.09 PS.seek 2.5ml @15:55:29.41 PSV.dial PTV @15:55:30.16 PBV.dial PSV @15:55:30.25 PS.seek 0ml @15:55:56.78 PSV.dial PBV @15:55:57.26 PS.seek 2.5ml @15:56:23.66 PSV.dial :waste1 @15:56:24.42 PS.seek 0ml @15:56:50.85 PSV.dial PSR @15:56:51.32 PSR.select :flush @15:56:51.44 PS.seek 1ml @15:57:02.53 PSR.select :air @15:57:02.65 PS.seek 2.5ml @15:57:19.00 PSV.dial PTV @15:57:19.75 PTV.dial :puck @15:57:19.83 PBV.dial :waste1 @15:57:20.40 PS.seek 0ml @15:57:46.78 PSV.dial PSR @15:57:47.55 PSR.select :flush @15:57:47.67 PS.seek 0.5ml @15:57:53.66 PSR.select :air @15:57:54.22 PS.seek 2.5ml @15:58:15.41 PSV.dial PTV @15:58:16.30 PBV.dial PSV @15:58:16.87 PS.seek 0ml @15:58:43.41 PSV.dial PBV @15:58:43.87 PS.seek 2.5ml @15:59:10.31 PSV.dial :waste1 @15:59:11.07 PS.seek 0ml @15:59:37.41 PSV.dial PSR @15:59:37.88 PSR.select :air @15:59:37.90 PS.seek 2.5ml @16:00:04.34 PSV.dial PTV @16:00:05.20 PBV.dial PSV @16:00:05.45 PS.seek 0ml @16:00:31.81 PSV.dial PBV @16:00:32.27 PS.seek 2.5ml @16:00:58.65 PSV.dial :waste1 @16:00:59.41 PS.seek 0ml @16:01:25.85
Loading FlushPuck into Collection Clamp from Processing Clamp @16:01:26.03 Elbow.seek PC @16:01:28.56 Elbow.reconfigure :loose @16:01:28.66 Forearm.seek PC @16:01:29.61 Hand.close @16:01:30.21 PC.seek :open @16:01:34.93 Forearm.seek :retract @16:01:35.88 Elbow.reconfigure :tight @16:01:35.93 Elbow.seek CC @16:01:39.56 Elbow.reconfigure :loose @16:01:39.66 Forearm.reconfigure :tight @16:01:39.70 Forearm.seek CC @16:01:40.94 CC.seek :closed @16:01:45.48 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.49 @16:01:45.55 Hand.open @16:01:46.27 Forearm.reconfigure :loose @16:01:46.31 Forearm.seek :retract @16:01:47.33 Flushing the Collection Valve::Manifold @16:01:47.45 CSV.dial CSR @16:01:47.90 Pulling 5ml of CSR.flush @16:01:47.91 CSR.select :flush @16:01:48.03 CS.seek 5ml @16:02:01.63 delay 5 seconds @16:02:06.65 CSR.select :air @16:02:06.82 CS.reconfigure :fast @16:02:06.87 CS.seek 7.5ml @16:02:13.69 CSV.dial :waste @16:02:14.99 CS.reconfigure :default @16:02:15.04 CS.seek 0ml @16:02:35.10 Flushing lower manifold's waste1 port @16:02:35.22 CSV.dial :flush @16:02:35.67 CS.reconfigure :fast @16:02:35.72 CS.seek 5ml @16:02:48.60 delay 5 seconds @16:02:53.62 CSV.dial :air @16:02:54.15 CS.seek 7ml @16:02:59.91 CSV.dial CTV @16:02:59.93
CTV.dial CSV @16:03:01.32 CBV.dial :waste1 @16:03:02.09 CS.seek 0ml @16:03:18.73 Flushing lower manifold's waste2 port @16:03:18.85 CSV.dial :flush @16:03:19.59 CS.seek 5ml @16:03:32.63 delay 5 seconds @16:03:37.65 CSV.dial :air @16:03:38.16 CS.seek 6.999ml @16:03:43.79 CSV.dial CTV @16:03:43.81
CTV.dial CSV @16:03:44.29 CBV.dial :waste2 @16:03:45.34 CS.seek 0ml @16:04:01.91 Flushing lower manifold's waste2b port @16:04:02.03 CSV.dial :flush @16:04:02.78 CS.seek 5ml @16:04:15.87 delay 5 seconds @16:04:20.91 CSV.dial :air @16:04:21.43 CS.seek 7ml @16:04:26.94 CSV.dial CTV @16:04:26.98
CTV.dial CSV @16:04:27.41 CBV.dial :waste2b @16:04:28.48 CS.seek 0ml @16:04:45.10 Disinfecting external Intake with kill @16:04:45.22 CSV.dial CSR @16:04:45.79 Pulling 8ml of CSR.kill CSR.select :kill @16:04:45.91 CS.seek 8ml @16:05:05.94 delay 5 seconds @16:05:10.96 CSR.select :air @16:05:11.08 CS.seek 10ml @16:05:16.70 CSV.dial CBV @16:05:16.72
CBV.dial CSV @16:05:17.92 CTV.dial :intake @16:05:18.69 Intake.select :open @16:05:18.81 CS.seek 2ml @16:05:37.72 Intake.select :close @16:05:37.83 CTV.dial :air @16:05:38.57 CS.reconfigure :default @16:05:38.62 CS.seek 0ml @16:05:44.53 CSV.dial CSR @16:05:45.53 Pulling 5ml of CSR.flush @16:05:45.54 CSR.select :flush @16:05:45.66 CS.seek 5ml @16:05:59.25 delay 5 seconds @16:06:04.27 CSR.select :air @16:06:04.39 CS.seek 10ml @16:06:18.06 CSV.dial CBV @16:06:19.09 CS.seek 0ml @16:06:45.57 Garaging FlushPuck in Collection Clamp @16:06:45.64 Elbow.reconfigure :tight @16:06:45.68 Elbow.seek CC @16:06:45.94 Elbow.reconfigure :loose @16:06:46.04 Forearm.seek CC @16:06:47.06 Hand.close @16:06:47.70 CC.seek :open @16:06:52.37 Forearm.seek :retract @16:06:53.39 Elbow.reconfigure :tight @16:06:53.44 Elbow.seek :garage @16:06:56.32 Elbow.reconfigure :loose @16:06:56.42 Forearm.reconfigure :tight @16:06:56.47 Forearm.seek :garage @16:06:57.46 Hand.open @16:06:58.14 Forearm.reconfigure :loose @16:06:58.19 Forearm.seek :retract @16:06:59.16 Completed [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] begun 13:37:45 @16:06:59.21 Asleep after completing DA_WCR_112723 assays @16:06:59.23 GoodNews.email "{1 => 33,\n 2 => 33,\n 3 => 6,\n 4 => 27,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@16:06:55, 30% flow, 17.1C, 27% humidity, 15.2psia, 13.725V, 0.345A, 93.092Ah, 4.73W\nSampled 425.0ml",Subject: "Asleep after completing DA_WCR_112723 assays" @16:07:03.14 upsync + ESPwhich DA_WCR_112723b.rb + fn=DA_WCR_112723b.rb + IFS=: + set /home/esp/esp2/mission . + fullPath=/home/esp/esp2/mission/DA_WCR_112723b.rb + [ -f /home/esp/esp2/mission/DA_WCR_112723b.rb ] + realpath /home/esp/esp2/mission/DA_WCR_112723b.rb + return 0 + scriptPath=/home/esp/esp2local/mission/DA_WCR_112723b.rb + exec ruby /home/esp/esp2/lib/esp.rb /home/esp/esp2local/mission/DA_WCR_112723b.rb @20:36:18.14UTC27-Nov-23
ESP2 v3.42 I2C Gateway v3.20 for core bus @20:36:20.75 Configuration for short puck jake 1/11/21 by brent@mbari.org /home/esp/esp2/type/shallow/jake/configure.rb @20:36:24.68 Polling Can every 10:00 @20:36:24.73 Cleanly exited at 20:31:53UTC27-Nov-23 @20:36:24.78 Loading /home/esp/esp2local/mission/DA_WCR_112723b.rb mission @20:36:24.84 Preparing to sleep until + 00:00:05 @20:36:24.85 GoodNews.email "Starting DA_WCR_112723b w/pucks in tube 4\n{1 => 33,\n 2 => 33,\n 3 => 6,\n 4 => 27,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@20:36:24, 30% flow, 17.2C, 29% humidity, 15.2psia, 13.720V, 0.302A, 94.409Ah, 4.15W",Subject: "DA_WCR_112723b begun" @20:36:24.89
Asleep til + 00:00:05 @20:36:24.94 Continuing at 20:36 @20:36:25.17 Gate.power :core,:OFF @20:36:25.19 Gate.power :raw,:OFF @20:36:25.20 Shutdown @20:36:28.01 GoodNews.email "Then, in tube 4: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_112723b.rb:14\n{1 => 33,\n 2 => 33,\n 3 => 6,\n 4 => 27,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@20:36:24, 30% flow, 17.2C, 29% humidity, 15.2psia, 13.720V, 0.302A, 94.409Ah, 4.15W",Subject: "Asleep until 20:36" @20:36:29.37 upsync @20:36:29.83
Powering core bus @20:36:32.18 upsync @20:36:35.18
Gate.power :raw,:ON @20:36:35.90 CS.configure :default @20:36:35.95 PS.configure :default @20:36:36.00 SS.configure :default @20:36:36.05 CC.configure :default @20:36:36.10 PC.configure :default @20:36:36.16 Forearm.configure :loose @20:36:36.21 Elbow.configure :loose @20:36:36.28 SC.configure :loose @20:36:36.33 SE.configure :default @20:36:36.38 CH.configure :default @20:36:36.42 PH.configure :default @20:36:36.46 All dwarf controllers running firmware version 3.85 @20:36:36.57 Valve.reset @20:36:37.18 Intake.select :close @20:36:37.23 Exhaust.select :close @20:36:37.27 CTV.dial :air @20:36:37.36 PSV.dial :air @20:36:37.39 CSV.dial :waste1 @20:36:37.55
Forearm.home.seek :retract @20:36:37.94 SSV.dial :spit,avoiding: :exhaust @20:36:38.28 Hand.close @20:36:38.31
Elbow.reconfigure :loose @20:36:38.41 Elbow.jog 1500 @20:36:38.50 PS.home.seek 0ml @20:36:38.82 CS.reconfigure :fast @20:36:38.86 SS.home.seek 0ml @20:36:38.95 CS.home.seek 0ml @20:36:39.02
Elbow.reconfigure :loose @20:36:39.08 Elbow.home.seek :clear @20:36:42.28 SE.home.seek :down @20:36:42.89 SC.reconfigure :tight @20:36:42.93 SC.home.seek :initial @20:36:46.82 PS.stop @20:36:58.68
SC.configure :limp @20:36:58.73 Forearm.seek :retract @20:36:58.80 Elbow.seek :hammer @20:36:59.83 Forearm.reconfigure :tight @20:36:59.87 Forearm.seek retracted @20:37:00.13 PC.home.seek :open @20:37:00.14
CC.home.seek :open @20:37:03.59 Phase [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] @20:37:03.61 Notice.email "At 20:37:03 began in tube 4: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_112723b.rb:14\n{1 => 33,\n 2 => 33,\n 3 => 6,\n 4 => 27,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@20:37:03, 30% flow, 17.2C, 29% humidity, 15.2psia, 13.725V, 0.296A, 94.413Ah, 4.07W",Subject: "Started DA_WCR_112723b phase initialPurge; da [1000, 1000] #da and wsr are run simultanelously" @20:37:03.64
Starting initialPurge @20:37:03.65 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @20:37:03.73 Hand.open @20:37:04.51 Elbow.reconfigure :tight @20:37:04.57 Elbow.seek FlushPuck::Garage @20:37:06.48 Elbow.reconfigure :loose @20:37:06.59 Forearm.reconfigure :loose @20:37:06.65 Forearm.seek :garage @20:37:07.68 Hand.close @20:37:08.10 upsync @20:37:08.39
Forearm.seek :retract @20:37:09.36 Elbow.reconfigure :tight @20:37:09.41 Elbow.seek CC @20:37:12.37 Elbow.reconfigure :loose @20:37:12.48 Forearm.reconfigure :tight @20:37:12.53 Forearm.seek CC @20:37:13.85 CC.seek :closed @20:37:18.44 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.38 @20:37:18.50 Hand.open @20:37:19.21 Forearm.reconfigure :loose @20:37:19.26 Forearm.seek :retract @20:37:20.25 Flushing the Collection Valve::Manifold @20:37:20.38 CSV.dial CSR @20:37:21.67 Pulling 5ml of CSR.flush @20:37:21.68 CSR.select :flush @20:37:21.80 CS.reconfigure :default @20:37:22.26 CS.seek 5ml @20:37:35.90 delay 5 seconds @20:37:40.92 CSR.select :air @20:37:41.10 CS.reconfigure :fast @20:37:41.14 CS.seek 7.501ml @20:37:47.93 CSV.dial :waste @20:37:49.23 CS.reconfigure :default @20:37:49.28 CS.seek 0ml @20:38:09.28 Purging external Intake @20:38:09.40 CSV.dial :flush @20:38:09.84 CS.reconfigure :fast @20:38:09.89 CS.seek 10ml @20:38:34.46 delay 5 seconds @20:38:39.49 CSV.dial CBV @20:38:39.52
CBV.dial CSV @20:38:40.26 CTV.dial :intake @20:38:41.06 Intake.select :open @20:38:41.16 CS.seek 0ml @20:39:04.68 Intake.select :close @20:39:04.79 CTV.dial :air @20:39:05.57 Flushing upper manifold @20:39:05.70 CSV.dial :flush @20:39:06.44 CS.seek 5ml @20:39:19.37 delay 5 seconds @20:39:24.39 CSV.dial :air @20:39:24.91 CS.seek 7.001ml @20:39:30.46 CSV.dial CBV @20:39:30.49
CBV.dial CSV @20:39:31.54 CTV.dial :air @20:39:31.63 CS.seek 0ml @20:39:48.37 delay 7 seconds @20:39:55.40 CS.seek 3ml @20:40:03.43 CSV.dial :waste @20:40:03.89 CS.seek 0ml @20:40:11.89 CSV.dial :flush @20:40:12.45 CS.seek 3ml @20:40:20.46 delay 5 seconds @20:40:25.48 CSV.dial :air @20:40:26.01 CS.seek 5ml @20:40:31.84 CSV.dial :air @20:40:32.01 CS.seek 0ml @20:40:44.12 delay 7 seconds @20:40:51.15 CS.seek 3ml @20:40:59.24 CSV.dial :waste @20:40:59.98 CS.seek 0ml @20:41:07.74 Garaging FlushPuck in Collection Clamp @20:41:07.82 Elbow.reconfigure :tight @20:41:07.87 Elbow.seek CC @20:41:08.19 Elbow.reconfigure :loose @20:41:08.30 Forearm.seek CC @20:41:09.35 Hand.close @20:41:10.02 CC.seek :open @20:41:15.04 Forearm.seek :retract @20:41:16.04 Elbow.reconfigure :tight @20:41:16.09 Elbow.seek :garage @20:41:19.05 Elbow.reconfigure :loose @20:41:19.15 Forearm.reconfigure :tight @20:41:19.20 Forearm.seek :garage @20:41:20.27 Hand.open @20:41:20.94 Forearm.reconfigure :loose @20:41:20.99 Forearm.seek :retract @20:41:21.97 da sampling at most 1000ml, wcr at most 1000ml @20:41:22.01 Loading sh1 puck from tube 4 into Collection Clamp @20:41:22.02 DAfans.email "Started da in tube 4 at 20:41:21UTC27-Nov-23\n{1 => 33,\n 2 => 33,\n 3 => 6,\n 4 => 27,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@20:37:03, 30% flow, 17.2C, 29% humidity, 15.2psia, 13.725V, 0.296A, 94.413Ah, 4.07W",Subject: "da sampling at most 1000ml, wcr at most 1000ml" @20:41:22.51
Elbow.reconfigure :tight @20:41:22.56 Elbow.seek 4 @20:41:24.54 Forearm.reconfigure :tight @20:41:24.59 Forearm.seek 4 + 450 counts @20:41:25.63 SE.reconfigure :pick @20:41:25.71 SE.seek :beamBreak @20:41:27.13 SE.configure :default @20:41:27.20 upsync @20:41:27.33
Elbow.seek 4 @20:41:27.53 Forearm.seek 4 + 300 counts @20:41:27.75 Forearm.reconfigure :loose @20:41:27.79 Hand.close @20:41:28.40 SE.seek :down @20:41:28.62
Forearm.seek :retract @20:41:29.81 SE.stop @20:41:29.93
Elbow.seek CC @20:41:31.55 Elbow.reconfigure :loose @20:41:31.65 Forearm.reconfigure :tight @20:41:31.70 Forearm.seek CC @20:41:32.98 CC.seek :closed @20:41:37.50 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.30 @20:41:37.51 Hand.open @20:41:38.20 Forearm.reconfigure :loose @20:41:38.24 Forearm.seek :retract @20:41:39.20 collectSample 1000ml w/bubble point of 24psi @20:41:39.21 Priming 7ml @20:41:39.22 Intake.select :close @20:41:39.23 Exhaust.select :close @20:41:39.25 SS.hold @20:41:39.45 Calibrating pressure and temperature sensors @20:41:39.48 CTV.dial :air @20:41:39.56 SSV.dial :spit,avoiding: :exhaust @20:41:39.63 SS.reconfigure :fast @20:41:39.68 SS.seek 5ml @20:41:42.82 SSV.dial CBV @20:41:42.84
CBV.dial SSV @20:41:44.19 delay 3.8 seconds @20:41:48.12 Sensor limits: Intake max 103.1psia; Outlet max 96.8psia SSV.dial :spit @20:41:49.45 SS.seek 0ml @20:41:52.08 SSV.dial CBV @20:41:53.40 CTV.dial :intake @20:41:54.20 SSV.dial CBV @20:41:54.22
CBV.dial SSV @20:41:54.41 Allowing 40s for 7.0ml of remaining 7.0ml @20:41:54.42 Intake.select :open @20:41:56.59 Intake @14.2psia, vacuum from 8.7 to 11.7psi @20:41:56.60 SS.reconfigure :inhaling 2.20ml/s @20:41:56.65 SS.seek 7ml @20:42:03.55 Stroke completed -- waiting for pressure to equalize ... @20:42:03.56 SS.reconfigure :exhale @20:42:03.60 SS.hold @20:42:13.71 Intake.select :close @20:42:13.86 SSV.dial :exhaust @20:42:14.63 Exhaust.select :open @20:42:14.74 SS.seek 0ml @20:42:19.00 Exhaust.select :close @20:42:19.11 Sampling 1000.0ml @20:42:19.12 Intake.select :close @20:42:19.13 Exhaust.select :close @20:42:19.14 SS.reconfigure :default @20:42:19.19 SS.hold @20:42:19.40 CTV.dial :intake @20:42:19.63 SSV.dial CBV @20:42:19.67
CBV.dial SSV @20:42:20.42 Allowing 130s for 25.0ml of remaining 1000.0ml @20:42:20.44 Intake.select :open @20:42:22.60 Intake @15.2psia, vacuum from 9.7 to 12.7psi @20:42:22.62 SS.reconfigure :inhaling 2.20ml/s @20:42:22.67 SS.seek 25ml @20:42:29.81 Vacuum too high -- waiting up to 40 seconds @20:42:29.82 SS.reconfigure :exhale @20:42:29.87 SS.hold @20:42:35.89 Intake @17.3psia, vacuum from 10.0 to 13.0psi @20:42:35.90 SS.reconfigure :inhaling 1.76ml/s @20:42:35.95 SS.seek 25ml @20:42:39.49 Vacuum too high -- waiting up to 40 seconds @20:42:39.51 SS.reconfigure :exhale @20:42:39.56 SS.hold @20:42:42.77 Intake @17.8psia, vacuum from 10.0 to 13.0psi @20:42:42.79 SS.reconfigure :inhaling 1.41ml/s @20:42:42.84 SS.seek 25ml @20:42:45.96 Vacuum too high -- waiting up to 40 seconds @20:42:45.98 SS.reconfigure :exhale @20:42:46.03 SS.hold @20:42:48.61 Intake @17.5psia, vacuum from 10.0 to 13.0psi @20:42:48.63 SS.reconfigure :inhaling 1.13ml/s @20:42:48.68 SS.seek 25ml @20:42:53.81 Vacuum too high -- waiting up to 40 seconds @20:42:53.82 SS.reconfigure :exhale @20:42:53.87 SS.hold @20:42:56.93 Intake @18.0psia, vacuum from 10.0 to 13.0psi @20:42:56.95 SS.reconfigure :inhaling 0.91ml/s @20:42:57.43 SS.seek 25ml @20:43:00.44 Stroke completed -- waiting for pressure to equalize ... @20:43:00.45 SS.reconfigure :exhale @20:43:00.50 SS.hold @20:43:29.07 Intake.select :close @20:43:29.23 SSV.dial :exhaust @20:43:29.99 Exhaust.select :open @20:43:30.10 SS.seek 0ml @20:43:38.91 Exhaust.select :close @20:43:39.01 SSV.dial CBV @20:43:39.04
CBV.dial SSV @20:43:39.81 Allowing 130s for 25.0ml of remaining 975.0ml @20:43:39.82 Intake.select :open @20:43:42.41 Intake @15.6psia, vacuum from 10.0 to 13.0psi @20:43:42.43 SS.reconfigure :inhaling 0.91ml/s @20:43:42.48 SS.seek 25ml @20:44:12.78 Stroke completed -- waiting for pressure to equalize ... @20:44:12.79 SS.reconfigure :exhale @20:44:12.84 SS.hold @20:44:45.51 Intake.select :close @20:44:45.66 SSV.dial :exhaust @20:44:46.44 Exhaust.select :open @20:44:46.59 SS.seek 0ml @20:44:55.34 Exhaust.select :close @20:44:55.88 SSV.dial CBV @20:44:55.91
CBV.dial SSV @20:44:56.69 Allowing 130s for 25.0ml of remaining 950.0ml @20:44:56.70 Intake.select :open @20:44:58.86 Intake @15.6psia, vacuum from 10.0 to 13.0psi @20:44:58.88 SS.reconfigure :inhaling 0.91ml/s @20:44:58.92 SS.seek 25ml @20:45:28.98 Stroke completed -- waiting for pressure to equalize ... @20:45:28.99 SS.reconfigure :exhale @20:45:29.04 SS.hold @20:46:01.71 Intake.select :close @20:46:02.29 SSV.dial :exhaust @20:46:03.05 Exhaust.select :open @20:46:03.16 SS.seek 0ml @20:46:11.98 Exhaust.select :close @20:46:12.09 SSV.dial CBV @20:46:12.11
CBV.dial SSV @20:46:12.90 Allowing 130s for 25.0ml of remaining 925.0ml @20:46:12.92 Intake.select :open @20:46:15.08 Intake @15.4psia, vacuum from 9.9 to 12.9psi @20:46:15.10 SS.reconfigure :inhaling 0.91ml/s @20:46:15.14 SS.seek 25ml @20:46:45.04 Stroke completed -- waiting for pressure to equalize ... @20:46:45.05 SS.reconfigure :exhale @20:46:45.10 SS.hold @20:47:13.67 Intake.select :close @20:47:14.25 SSV.dial :exhaust @20:47:15.03 Exhaust.select :open @20:47:15.14 SS.seek 0ml @20:47:23.87 Exhaust.select :close @20:47:23.98 SSV.dial CBV @20:47:24.00
CBV.dial SSV @20:47:24.79 Allowing 130s for 25.0ml of remaining 900.0ml @20:47:24.80 Intake.select :open @20:47:26.97 Intake @15.6psia, vacuum from 10.0 to 13.0psi @20:47:26.98 SS.reconfigure :inhaling 0.91ml/s @20:47:27.03 SS.seek 25ml @20:47:57.07 Stroke completed -- waiting for pressure to equalize ... @20:47:57.08 SS.reconfigure :exhale @20:47:57.13 SS.hold @20:48:21.61 Intake.select :close @20:48:21.77 SSV.dial :exhaust @20:48:22.53 Exhaust.select :open @20:48:23.01 SS.seek 0ml @20:48:31.78 Exhaust.select :close @20:48:31.88 SSV.dial CBV @20:48:31.90
CBV.dial SSV @20:48:32.71 Allowing 130s for 25.0ml of remaining 875.0ml @20:48:32.73 Intake.select :open @20:48:34.90 Intake @15.7psia, vacuum from 10.0 to 13.0psi @20:48:34.91 SS.reconfigure :inhaling 0.91ml/s @20:48:34.96 SS.seek 25ml @20:49:04.90 Stroke completed -- waiting for pressure to equalize ... @20:49:04.91 SS.reconfigure :exhale @20:49:04.96 SS.hold @20:49:29.38 Intake.select :close @20:49:29.53 SSV.dial :exhaust @20:49:30.31 Exhaust.select :open @20:49:30.42 SS.seek 0ml @20:49:39.60 Exhaust.select :close @20:49:39.71 SSV.dial CBV @20:49:40.09
CBV.dial SSV @20:49:40.95 Allowing 130s for 25.0ml of remaining 850.0ml @20:49:40.96 Intake.select :open @20:49:43.12 Intake @15.5psia, vacuum from 10.0 to 13.0psi @20:49:43.14 SS.reconfigure :inhaling 0.91ml/s @20:49:43.19 SS.seek 25ml @20:50:12.66 Stroke completed -- waiting for pressure to equalize ... @20:50:12.67 SS.reconfigure :exhale @20:50:12.72 SS.hold @20:50:33.57 Intake.select :close @20:50:33.72 SSV.dial :exhaust @20:50:34.48 Exhaust.select :open @20:50:34.60 SS.seek 0ml @20:50:43.54 Exhaust.select :close @20:50:43.65 SSV.dial CBV @20:50:43.67
CBV.dial SSV @20:50:44.45 Allowing 130s for 25.0ml of remaining 825.0ml @20:50:44.46 Intake.select :open @20:50:46.62 Intake @15.7psia, vacuum from 10.0 to 13.0psi @20:50:46.63 SS.reconfigure :inhaling 0.91ml/s @20:50:46.68 SS.seek 25ml @20:51:16.04 Stroke completed -- waiting for pressure to equalize ... @20:51:16.05 SS.reconfigure :exhale @20:51:16.45 SS.hold @20:51:36.95 Intake.select :close @20:51:37.10 SSV.dial :exhaust @20:51:37.88 Exhaust.select :open @20:51:38.00 SS.seek 0ml @20:51:46.97 Exhaust.select :close @20:51:47.09 SSV.dial CBV @20:51:47.11
CBV.dial SSV @20:51:47.90 Allowing 130s for 25.0ml of remaining 800.0ml @20:51:47.91 Intake.select :open @20:51:50.08 Intake @15.6psia, vacuum from 10.0 to 13.0psi @20:51:50.09 SS.reconfigure :inhaling 0.91ml/s @20:51:50.14 SS.seek 25ml @20:52:19.46 Stroke completed -- waiting for pressure to equalize ... @20:52:19.47 SS.reconfigure :exhale @20:52:19.51 SS.hold @20:52:40.17 Intake.select :close @20:52:40.33 SSV.dial :exhaust @20:52:41.09 Exhaust.select :open @20:52:41.21 SS.seek 0ml @20:52:50.18 Exhaust.select :close @20:52:50.28 SSV.dial CBV @20:52:50.32
CBV.dial SSV @20:52:51.08 Allowing 130s for 25.0ml of remaining 775.0ml @20:52:51.09 Intake.select :open @20:52:53.26 Intake @15.7psia, vacuum from 10.0 to 13.0psi @20:52:53.28 SS.reconfigure :inhaling 0.91ml/s @20:52:53.33 SS.seek 25ml @20:53:22.60 Stroke completed -- waiting for pressure to equalize ... @20:53:22.61 SS.reconfigure :exhale @20:53:22.65 SS.hold @20:53:43.55 Intake.select :close @20:53:43.71 SSV.dial :exhaust @20:53:44.48 Exhaust.select :open @20:53:44.59 SS.seek 0ml @20:53:53.35 Exhaust.select :close @20:53:53.46 SSV.dial CBV @20:53:53.48
CBV.dial SSV @20:53:54.29 Allowing 130s for 25.0ml of remaining 750.0ml @20:53:54.30 Intake.select :open @20:53:56.46 Intake @15.6psia, vacuum from 10.0 to 13.0psi @20:53:56.48 SS.reconfigure :inhaling 0.91ml/s @20:53:56.53 SS.seek 25ml @20:54:26.47 Stroke completed -- waiting for pressure to equalize ... @20:54:26.48 SS.reconfigure :exhale @20:54:26.53 SS.hold @20:54:49.03 Intake.select :close @20:54:49.19 SSV.dial :exhaust @20:54:49.94 Exhaust.select :open @20:54:50.06 SS.seek 0ml @20:54:58.83 Exhaust.select :close @20:54:58.94 SSV.dial CBV @20:54:58.97
CBV.dial SSV @20:54:59.76 Allowing 130s for 25.0ml of remaining 725.0ml @20:54:59.77 Intake.select :open @20:55:01.93 Intake @15.6psia, vacuum from 10.0 to 13.0psi @20:55:01.94 SS.reconfigure :inhaling 0.91ml/s @20:55:01.99 SS.seek 25ml @20:55:32.06 Stroke completed -- waiting for pressure to equalize ... @20:55:32.07 SS.reconfigure :exhale @20:55:32.12 SS.hold @20:55:52.52 Intake.select :close @20:55:52.68 SSV.dial :exhaust @20:55:53.45 Exhaust.select :open @20:55:53.56 SS.seek 0ml @20:56:02.25 Exhaust.select :close @20:56:02.36 SSV.dial CBV @20:56:02.38
CBV.dial SSV @20:56:03.16 Allowing 130s for 25.0ml of remaining 700.0ml @20:56:03.17 Intake.select :open @20:56:05.34 Intake @15.6psia, vacuum from 10.0 to 13.0psi @20:56:05.35 SS.reconfigure :inhaling 0.91ml/s @20:56:05.40 SS.seek 25ml @20:56:35.39 Stroke completed -- waiting for pressure to equalize ... @20:56:35.40 SS.reconfigure :exhale @20:56:35.45 SS.hold @20:56:57.90 Intake.select :close @20:56:58.05 SSV.dial :exhaust @20:56:58.82 Exhaust.select :open @20:56:58.93 SS.seek 0ml @20:57:07.53 Exhaust.select :close @20:57:07.65 SSV.dial CBV @20:57:07.68
CBV.dial SSV @20:57:08.42 Allowing 130s for 25.0ml of remaining 675.0ml @20:57:08.43 Intake.select :open @20:57:10.60 Intake @15.6psia, vacuum from 10.0 to 13.0psi @20:57:10.61 SS.reconfigure :inhaling 0.91ml/s @20:57:10.66 SS.seek 25ml @20:57:40.39 Stroke completed -- waiting for pressure to equalize ... @20:57:40.40 SS.reconfigure :exhale @20:57:40.45 SS.hold @20:58:04.87 Intake.select :close @20:58:05.03 SSV.dial :exhaust @20:58:05.81 Exhaust.select :open @20:58:05.93 SS.seek 0ml @20:58:14.70 Exhaust.select :close @20:58:14.82 SSV.dial CBV @20:58:14.84
CBV.dial SSV @20:58:15.64 Allowing 130s for 25.0ml of remaining 650.0ml @20:58:15.65 Intake.select :open @20:58:17.82 Intake @15.6psia, vacuum from 10.0 to 13.0psi @20:58:17.83 SS.reconfigure :inhaling 0.91ml/s @20:58:17.88 SS.seek 25ml @20:58:47.59 Stroke completed -- waiting for pressure to equalize ... @20:58:47.60 SS.reconfigure :exhale @20:58:47.65 SS.hold @20:59:12.56 Intake.select :close @20:59:12.71 SSV.dial :exhaust @20:59:13.47 Exhaust.select :open @20:59:13.58 SS.seek 0ml @20:59:22.56 Exhaust.select :close @20:59:22.67 SSV.dial CBV @20:59:22.69
CBV.dial SSV @20:59:23.48 Allowing 130s for 25.0ml of remaining 625.0ml @20:59:23.49 Intake.select :open @20:59:25.66 Intake @15.5psia, vacuum from 10.0 to 13.0psi @20:59:25.68 SS.reconfigure :inhaling 0.91ml/s @20:59:25.72 SS.seek 25ml @20:59:54.04 Vacuum too high -- waiting up to 40 seconds @20:59:54.05 SS.reconfigure :exhale @20:59:54.10 SS.hold @21:00:08.64 Intake @18.2psia, vacuum from 10.0 to 13.0psi @21:00:08.66 SS.reconfigure :inhaling 0.73ml/s @21:00:08.70 SS.seek 25ml @21:00:10.17 Stroke completed -- waiting for pressure to equalize ... @21:00:10.18 SS.reconfigure :exhale @21:00:10.22 SS.hold @21:00:23.33 Intake.select :close @21:00:23.49 SSV.dial :exhaust @21:00:24.25 Exhaust.select :open @21:00:24.36 SS.seek 0ml @21:00:33.36 Exhaust.select :close @21:00:33.46 SSV.dial CBV @21:00:33.48
CBV.dial SSV @21:00:34.28 Allowing 130s for 25.0ml of remaining 600.0ml @21:00:34.29 Intake.select :open @21:00:36.46 Intake @15.5psia, vacuum from 10.0 to 13.0psi @21:00:36.47 SS.reconfigure :inhaling 0.73ml/s @21:00:36.52 SS.seek 25ml @21:01:12.31 Stroke completed -- waiting for pressure to equalize ... @21:01:12.32 SS.reconfigure :exhale @21:01:12.37 SS.hold @21:01:36.75 Intake.select :close @21:01:37.66 SSV.dial :exhaust @21:01:38.43 Exhaust.select :open @21:01:38.55 SS.seek 0ml @21:01:47.59 Exhaust.select :close @21:01:47.70 SSV.dial CBV @21:01:47.72
CBV.dial SSV @21:01:48.54 Allowing 130s for 25.0ml of remaining 575.0ml @21:01:48.55 Intake.select :open @21:01:50.72 Intake @15.5psia, vacuum from 10.0 to 13.0psi @21:01:50.74 SS.reconfigure :inhaling 0.73ml/s @21:01:50.79 SS.seek 25ml @21:02:26.79 Stroke completed -- waiting for pressure to equalize ... @21:02:26.80 SS.reconfigure :exhale @21:02:26.85 SS.hold @21:02:51.36 Intake.select :close @21:02:51.52 SSV.dial :exhaust @21:02:52.28 Exhaust.select :open @21:02:52.39 SS.seek 0ml @21:03:01.15 Exhaust.select :close @21:03:01.26 SSV.dial CBV @21:03:01.28
CBV.dial SSV @21:03:02.10 Allowing 130s for 25.0ml of remaining 550.0ml @21:03:02.12 Intake.select :open @21:03:04.28 Intake @15.6psia, vacuum from 10.0 to 13.0psi @21:03:04.29 SS.reconfigure :inhaling 0.73ml/s @21:03:04.34 SS.seek 25ml @21:03:40.65 Stroke completed -- waiting for pressure to equalize ... @21:03:40.66 SS.reconfigure :exhale @21:03:40.71 SS.hold @21:04:07.28 Intake.select :close @21:04:07.43 SSV.dial :exhaust @21:04:08.19 Exhaust.select :open @21:04:08.35 SS.seek 0ml @21:04:17.13 Exhaust.select :close @21:04:17.24 SSV.dial CBV @21:04:17.28
CBV.dial SSV @21:04:18.04 Allowing 130s for 25.0ml of remaining 525.0ml @21:04:18.05 Intake.select :open @21:04:20.22 Intake @15.6psia, vacuum from 10.0 to 13.0psi @21:04:20.24 SS.reconfigure :inhaling 0.73ml/s @21:04:20.29 SS.seek 25ml @21:04:56.96 Stroke completed -- waiting for pressure to equalize ... @21:04:56.97 SS.reconfigure :exhale @21:04:57.02 SS.hold @21:05:25.78 Intake.select :close @21:05:25.93 SSV.dial :exhaust @21:05:26.70 Exhaust.select :open @21:05:26.81 SS.seek 0ml @21:05:35.62 Exhaust.select :close @21:05:35.72 SSV.dial CBV @21:05:35.76
CBV.dial SSV @21:05:36.52 Allowing 130s for 25.0ml of remaining 500.0ml @21:05:36.54 Intake.select :open @21:05:38.70 Intake @15.7psia, vacuum from 10.0 to 13.0psi @21:05:38.72 SS.reconfigure :inhaling 0.73ml/s @21:05:38.76 SS.seek 25ml @21:06:15.29 Stroke completed -- waiting for pressure to equalize ... @21:06:15.30 SS.reconfigure :exhale @21:06:15.34 SS.hold @21:06:45.94 Intake.select :close @21:06:46.09 SSV.dial :exhaust @21:06:46.85 Exhaust.select :open @21:06:46.97 SS.seek 0ml @21:06:55.80 Exhaust.select :close @21:06:55.91 SSV.dial CBV @21:06:55.94
CBV.dial SSV @21:06:56.73 Allowing 130s for 25.0ml of remaining 475.0ml @21:06:56.74 Intake.select :open @21:06:58.90 Intake @15.7psia, vacuum from 10.0 to 13.0psi @21:06:58.91 SS.reconfigure :inhaling 0.73ml/s @21:06:58.96 SS.seek 25ml @21:07:33.00 Vacuum too high -- waiting up to 40 seconds @21:07:33.01 SS.reconfigure :exhale @21:07:33.06 SS.hold @21:07:51.76 Intake @18.1psia, vacuum from 10.0 to 13.0psi @21:07:51.78 SS.reconfigure :inhaling 0.59ml/s @21:07:51.83 SS.seek 25ml @21:07:55.00 Stroke completed -- waiting for pressure to equalize ... @21:07:55.01 SS.reconfigure :exhale @21:07:55.06 SS.hold @21:08:09.86 Intake.select :close @21:08:10.01 SSV.dial :exhaust @21:08:10.77 Exhaust.select :open @21:08:10.89 SS.seek 0ml @21:08:19.68 Exhaust.select :close @21:08:19.78 SSV.dial CBV @21:08:19.80
CBV.dial SSV @21:08:20.62 Allowing 130s for 25.0ml of remaining 450.0ml @21:08:20.63 Intake.select :open @21:08:22.79 Intake @15.6psia, vacuum from 10.0 to 13.0psi @21:08:22.80 SS.reconfigure :inhaling 0.59ml/s @21:08:22.85 SS.seek 25ml @21:09:07.80 Stroke completed -- waiting for pressure to equalize ... @21:09:07.81 SS.reconfigure :exhale @21:09:07.86 SS.hold @21:09:38.48 Intake.select :close @21:09:38.63 SSV.dial :exhaust @21:09:39.40 Exhaust.select :open @21:09:39.51 SS.seek 0ml @21:09:48.28 Exhaust.select :close @21:09:48.39 SSV.dial CBV @21:09:48.42
CBV.dial SSV @21:09:49.19 Allowing 130s for 25.0ml of remaining 425.0ml @21:09:49.20 Intake.select :open @21:09:51.37 Intake @15.6psia, vacuum from 10.0 to 13.0psi @21:09:51.39 SS.reconfigure :inhaling 0.59ml/s @21:09:51.44 SS.seek 25ml @21:10:36.06 Stroke completed -- waiting for pressure to equalize ... @21:10:36.07 SS.reconfigure :exhale @21:10:36.12 SS.hold @21:11:08.90 Intake.select :close @21:11:09.05 SSV.dial :exhaust @21:11:09.82 Exhaust.select :open @21:11:09.94 SS.seek 0ml @21:11:18.78 Exhaust.select :close @21:11:18.89 SSV.dial CBV @21:11:18.92
CBV.dial SSV @21:11:19.69 Allowing 130s for 25.0ml of remaining 400.0ml @21:11:19.70 Intake.select :open @21:11:21.87 Intake @15.7psia, vacuum from 10.0 to 13.0psi @21:11:21.89 SS.reconfigure :inhaling 0.59ml/s @21:11:21.93 SS.seek 25ml @21:12:06.61 Stroke completed -- waiting for pressure to equalize ... @21:12:06.62 SS.reconfigure :exhale @21:12:06.67 SS.hold @21:12:43.85 Intake.select :close @21:12:44.00 SSV.dial :exhaust @21:12:44.77 Exhaust.select :open @21:12:44.88 SS.seek 0ml @21:12:53.70 Exhaust.select :close @21:12:53.81 SSV.dial CBV @21:12:53.83
CBV.dial SSV @21:12:54.63 Allowing 130s for 25.0ml of remaining 375.0ml @21:12:54.64 Intake.select :open @21:12:56.80 Intake @15.7psia, vacuum from 10.0 to 13.0psi @21:12:56.81 SS.reconfigure :inhaling 0.59ml/s @21:12:56.86 SS.seek 25ml @21:13:41.67 Stroke completed -- waiting for pressure to equalize ... @21:13:41.68 SS.reconfigure :exhale @21:13:41.73 SS.hold @21:14:21.91 Intake.select :close @21:14:22.06 SSV.dial :exhaust @21:14:22.83 Exhaust.select :open @21:14:22.94 SS.seek 0ml @21:14:31.94 Exhaust.select :close @21:14:32.04 SSV.dial CBV @21:14:32.07
CBV.dial SSV @21:14:32.84 Allowing 130s for 25.0ml of remaining 350.0ml @21:14:32.85 Intake.select :open @21:14:35.02 Intake @15.6psia, vacuum from 10.0 to 13.0psi @21:14:35.03 SS.reconfigure :inhaling 0.59ml/s @21:14:35.08 SS.seek 25ml @21:15:17.36 Vacuum too high -- waiting up to 40 seconds @21:15:17.38 SS.reconfigure :exhale @21:15:17.86 SS.hold @21:15:44.37 Intake @18.4psia, vacuum from 10.0 to 13.0psi @21:15:44.39 SS.reconfigure :inhaling 0.47ml/s @21:15:44.44 SS.seek 25ml @21:15:47.62 Stroke completed -- waiting for pressure to equalize ... @21:15:47.63 SS.reconfigure :exhale @21:15:47.68 SS.hold @21:16:06.35 Intake.select :close @21:16:06.50 SSV.dial :exhaust @21:16:07.28 Exhaust.select :open @21:16:07.39 SS.seek 0ml @21:16:16.23 Exhaust.select :close @21:16:16.34 SSV.dial CBV @21:16:16.36
CBV.dial SSV @21:16:17.17 Allowing 130s for 25.0ml of remaining 325.0ml @21:16:17.18 Intake.select :open @21:16:19.35 Intake @15.6psia, vacuum from 10.0 to 13.0psi @21:16:19.37 SS.reconfigure :inhaling 0.47ml/s @21:16:19.41 SS.seek 25ml @21:17:15.10 Stroke completed -- waiting for pressure to equalize ... @21:17:15.11 SS.reconfigure :exhale @21:17:15.16 SS.hold @21:17:51.92 Intake.select :close @21:17:52.07 SSV.dial :exhaust @21:17:52.82 Exhaust.select :open @21:17:52.94 SS.seek 0ml @21:18:01.70 Exhaust.select :close @21:18:01.80 SSV.dial CBV @21:18:01.83
CBV.dial SSV @21:18:02.64 Allowing 130s for 25.0ml of remaining 300.0ml @21:18:02.65 Intake.select :open @21:18:04.82 Intake @15.6psia, vacuum from 10.0 to 13.0psi @21:18:04.83 SS.reconfigure :inhaling 0.47ml/s @21:18:04.88 SS.seek 25ml @21:19:00.71 Stroke completed -- waiting for pressure to equalize ... @21:19:00.72 SS.reconfigure :exhale @21:19:00.77 SS.hold @21:19:40.95 SS.reconfigure :default @21:19:41.00 SS.hold @21:19:41.06 Intake.select :close @21:19:41.22 ShallowSampler::Clogged in MAIN -- Intake @16psia & Outlet @14psia won't equalize after 725.0ml @21:19:41.23 SSV.dial :exhaust @21:19:41.99 Exhaust.select :open @21:19:42.53 SS.reconfigure :exhale @21:19:42.58 SS.seek 0ml @21:19:51.68 Exhaust.select :close @21:19:51.79 Sampled 725.0ml @21:19:51.81 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @21:19:51.82 Intake.select :close @21:19:51.88 CSV.dial :waste @21:19:52.00 SSV.dial CBV,avoiding: :exhaust @21:19:52.03
CBV.dial SSV @21:19:52.16 CS.seek 0ml @21:19:52.24 CSV.dial :air @21:19:52.98 CS.seek 10ml @21:19:53.95
CTV.dial :air @21:19:54.71 delay 15 seconds @21:20:09.73 SSV.dial :spit,avoiding: :exhaust @21:20:11.06 CTV.dial CSV,avoiding: :intake @21:20:11.09
SS.seek 0ml @21:20:11.22 SSV.dial CBV,avoiding: :exhaust @21:20:17.65 SSV.dial between CBV and plug @21:20:18.14 CBV.dial between SSV and waste2 @21:20:18.54 CTV.dial CSV,avoiding: :air @21:20:18.56
CSV.dial CTV @21:20:19.06 CS.reconfigure :pressurize @21:20:19.11 CS.seek 0ml @21:20:37.00 BubblePoint: Intake @24.1psia, Outlet @0.9psia @21:20:37.01 CBV.dial SSV @21:20:37.34 SS.reconfigure :default @21:20:37.38 SS.seek 10ml @21:20:42.86 delay 15 seconds @21:20:57.99 Evacuated: Intake @23.6psia, Outlet @23.5psia @21:20:58.00 SSV.dial :spit,avoiding: :exhaust @21:20:59.04 SS.reconfigure :exhale @21:20:59.09 SS.seek 0ml @21:21:04.10 SS.reconfigure :fast @21:21:04.15 SS.seek 5ml @21:21:06.98 SSV.dial CBV,avoiding: :exhaust @21:21:07.00
CBV.dial SSV @21:21:08.38 CTV.dial :air,avoiding: :intake @21:21:09.72 CSV.dial CTV @21:21:09.77
SS.seek 10ml @21:21:10.88 CSV.dial :air @21:21:11.34 CS.reconfigure :fast @21:21:11.39 CS.seek 0ml @21:21:12.40
SSV.dial :spit,avoiding: :exhaust @21:21:13.73 SS.reconfigure :exhale @21:21:13.78 SS.seek 0ml @21:21:19.17 SS.reconfigure :fast @21:21:19.22 SS.seek 5ml @21:21:19.58 CS.stop @21:21:22.14 SSV.dial CBV,avoiding: :exhaust @21:21:22.16
CBV.dial SSV @21:21:24.50 SSV.dial :spit,avoiding: :exhaust @21:21:25.84 SS.reconfigure :default @21:21:25.89 SS.seek 0ml @21:21:29.00 SS.stop @21:21:29.05 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @21:21:29.06 Intake.select :close @21:21:29.12 CSV.dial :waste @21:21:29.25 SSV.dial CBV,avoiding: :exhaust @21:21:29.27
CBV.dial SSV @21:21:29.90 CS.seek 0ml @21:21:29.96 CSV.dial :air @21:21:30.60
CTV.dial :air @21:21:30.70 CS.seek 10ml @21:21:30.77
delay 15 seconds @21:21:45.79 SSV.dial :spit,avoiding: :exhaust @21:21:47.12 CTV.dial CSV,avoiding: :intake @21:21:47.15
SS.reconfigure :exhale @21:21:47.26 SS.seek 0ml @21:21:47.65 SSV.dial CBV,avoiding: :exhaust @21:21:55.21 SSV.dial between CBV and plug @21:21:55.70 CBV.dial between SSV and waste2 @21:21:56.09 CSV.dial between air and CTV @21:21:56.42 CS.seek 7.788ml @21:22:02.56 CTV.dial CSV,avoiding: :air @21:22:02.58
CSV.dial CTV @21:22:02.95 CS.reconfigure :pressurize @21:22:03.00 CS.seek 0ml @21:22:16.96 BubblePoint: Intake @24.2psia, Outlet @3.4psia @21:22:16.97 CBV.dial SSV @21:22:17.32 SS.reconfigure :default @21:22:17.37 SS.seek 10ml @21:22:22.93 delay 15 seconds @21:22:38.06 Evacuated: Intake @23.9psia, Outlet @24.2psia @21:22:38.07 SSV.dial :spit,avoiding: :exhaust @21:22:39.10 SS.reconfigure :exhale @21:22:39.15 SS.seek 0ml @21:22:44.16 SS.reconfigure :fast @21:22:44.21 SS.seek 5ml @21:22:46.96 SSV.dial CBV,avoiding: :exhaust @21:22:46.99
CBV.dial SSV @21:22:48.41 CTV.dial :air,avoiding: :intake @21:22:49.75 CSV.dial CTV @21:22:49.81
SS.seek 10ml @21:22:50.96 CSV.dial :air @21:22:51.41 CS.reconfigure :fast @21:22:51.46 CS.seek 0ml @21:22:52.33
SSV.dial :spit,avoiding: :exhaust @21:22:53.68 SS.reconfigure :exhale @21:22:53.73 SS.seek 0ml @21:22:58.17 CS.stop @21:22:58.76
SS.reconfigure :fast @21:22:58.81 SS.seek 5ml @21:23:01.76 SSV.dial CBV,avoiding: :exhaust @21:23:01.78
CBV.dial SSV @21:23:04.14 SSV.dial :spit,avoiding: :exhaust @21:23:05.46 SS.reconfigure :default @21:23:05.51 SS.seek 0ml @21:23:08.63 SS.stop @21:23:08.67 Lysing: Adding 2.0ml AqMeOH @21:23:08.68 CBV.dial between CSV and waste1,avoiding: :DBV @21:23:10.19 CTV.dial CSV @21:23:11.50 CSV.dial CSR @21:23:12.29 Pulling 1ml of CSR.AqMeOH @21:23:12.30 CSR.select :AqMeOH @21:23:12.41 CS.reconfigure :default @21:23:12.46 CS.seek 1ml @21:23:16.11 delay 5 seconds @21:23:21.13 CSR.select :air @21:23:21.30 CS.seek 6ml @21:23:34.86 delay 2 seconds @21:23:36.88 CSV.dial :waste2 @21:23:37.33 CS.reconfigure :fast @21:23:37.38 CS.seek 0ml @21:23:51.86 CSV.dial CSR @21:23:52.31 Pulling 2ml of CSR.AqMeOH @21:23:52.32 CSR.select :AqMeOH @21:23:52.43 CS.reconfigure :default @21:23:52.48 CS.seek 2ml @21:23:58.45 delay 5 seconds @21:24:03.47 CSR.select :air @21:24:03.64 CS.seek 5ml @21:24:12.30 delay 2 seconds @21:24:14.31 CSV.dial CTV @21:24:14.77 CS.seek 1ml @21:24:26.12 Before lysing Collection Puck Heater at 13.8C/31.0psia @21:24:26.14 Heating Puck.from 4, :sh1 in Collection Clamp to 70C for 10:00 @21:24:26.16 CH.seek 70C,8:00 @21:26:38.24 CH.hold 10:00,70C @21:26:38.43 Begun lysing Collection Puck Heater at 68.1C/38.3psia @21:36:38.44 Ended lysing Collection Puck Heater at 70C/37.2psia @21:36:38.46 CS.seek 0ml @21:36:42.10 CTV.dial between spare and air,avoiding: :intake @21:36:43.13 CSV.dial :air @21:36:43.58 CS.seek 5ml @21:36:57.19 CSV.dial CTV @21:36:57.64 delay 2 seconds @21:36:59.66 CSV.dial :air @21:37:00.11 delay 2 seconds @21:37:02.13 CSV.dial CTV @21:37:02.59 delay 2 seconds @21:37:04.61 CSV.dial :air @21:37:05.05 delay 2 seconds @21:37:07.07 CS.seek 0ml @21:37:20.68 Puck @81.2psia after final lysis pressurization @21:37:20.69 Recovering Lysate into Collection syringe @21:37:20.70 CSV.dial CBV @21:37:21.71 CBV.dial CSV @21:37:22.09 CS.seek 5ml @21:37:35.69 delay 5 seconds @21:37:40.71 CTV.dial :air @21:37:41.23 CS.seek 7.001ml @21:37:47.38 delay 5 seconds @21:37:52.40 CSV.dial :air2 @21:37:52.84 CS.seek 10ml @21:38:01.44 CSV.dial between air and CTV,avoiding: CBV @21:38:02.60 Lysing Complete @21:38:02.67 Storing sh1 puck in Collection Clamp to tube 3 @21:38:02.84 SC.reconfigure :tight @21:38:02.89 SC.seek 3 @21:38:06.47 SC.configure :limp @21:38:06.68 Elbow.reconfigure :tight @21:38:06.73 Elbow.seek 3 @21:38:08.52 Forearm.reconfigure :tight @21:38:08.57 Forearm.seek 3 + 450 counts @21:38:09.60 SE.reconfigure :pick @21:38:10.07 SE.seek :beamBreak @21:38:12.90 SE.configure :default @21:38:13.07 Elbow.reconfigure :limp @21:38:13.12 Forearm.reconfigure :loose @21:38:13.16 Forearm.seek 3 @21:38:13.36 Elbow.hold @21:38:13.42 Elbow.reconfigure :tight @21:38:13.52 SE.seek 5.18 Pucks below Top Plate @21:38:13.96 Forearm.force -20 @21:38:14.00 Forearm.seek 3 + 298 counts @21:38:14.29 Forearm.reconfigure :tight @21:38:14.34 Forearm.seek 3 + 450 counts @21:38:14.54 SE.seek 6.18 Pucks below Top Plate @21:38:15.13 Forearm.reconfigure :loose @21:38:15.18 Forearm.seek :retract @21:38:16.11 Elbow.seek CC @21:38:17.80 Elbow.reconfigure :loose @21:38:17.91 Forearm.seek CC @21:38:18.89 Hand.close @21:38:19.47 CC.seek :open @21:38:24.20 Forearm.seek :retract @21:38:25.14 Elbow.reconfigure :tight @21:38:25.19 Elbow.seek 3 @21:38:26.94 Elbow.reconfigure :limp @21:38:26.99 Forearm.seek 3 @21:38:28.01 Elbow.hold @21:38:28.08 Elbow.reconfigure :tight @21:38:28.13 Hand.open @21:38:28.93 Forearm.force -20 @21:38:29.35 Forearm.seek 3 + 318 counts @21:38:29.71 Forearm.reconfigure :tight @21:38:29.76 Forearm.seek 3 + 450 counts @21:38:30.02 SE.seek :down @21:38:31.15
Forearm.reconfigure :loose @21:38:31.19 Forearm.seek :retract @21:38:32.32 SE.stop @21:38:32.36
Tranferring DA @21:38:32.37 Beginning samplePassC @21:38:32.38 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @21:38:32.57 Elbow.seek FlushPuck::Garage @21:38:34.38 Elbow.reconfigure :loose @21:38:34.49 Forearm.seek :garage @21:38:35.47 Hand.close @21:38:36.21 Forearm.seek :retract @21:38:37.20 Elbow.reconfigure :tight @21:38:37.25 Elbow.seek CC @21:38:40.21 Elbow.reconfigure :loose @21:38:40.31 Forearm.reconfigure :tight @21:38:40.36 Forearm.seek CC @21:38:41.65 CC.seek :closed @21:38:46.19 Collection Clamp onSpring/offSpring for 12/26 samples, on/off torque 2.36 @21:38:46.26 Hand.open @21:38:47.35 Forearm.reconfigure :loose @21:38:47.40 Forearm.seek :retract @21:38:48.39 PSV.dial PSR @21:38:49.78 Pulling 1.5ml of PSR.flush @21:38:49.79 PSR.select :flush @21:38:49.91 PS.seek 1.5ml @21:39:06.13 delay 5 seconds @21:39:11.15 PSV.dial :waste1 @21:39:11.62 PS.seek 0ml @21:39:27.72 PSV.dial PSR @21:39:28.19 PSR.select :air @21:39:28.31 PS.seek 2.5ml @21:39:54.73 PRV.dial CBV,avoiding: :ab2 @21:39:54.75
CBV.dial PRV @21:39:56.51 PSV.dial :mixloop @21:39:57.55 CTV.dial :air,avoiding: :intake @21:39:57.62 PTV.dial :PRVmixing @21:39:58.95 PS.seek 0ml @21:40:25.35 PSV.dial :air @21:40:25.82 PS.seek 2.5ml @21:40:52.10 PSV.dial :mixloop @21:40:52.59 PS.seek 0ml @21:41:18.97 PSV.dial :air @21:41:19.45 PS.seek 2.5ml @21:41:45.81 PSV.dial :mixloop @21:41:46.29 PS.seek 0ml @21:42:12.57 Garaging FlushPuck in Collection Clamp @21:42:12.64 Elbow.reconfigure :tight @21:42:12.69 Elbow.seek CC @21:42:12.99 Elbow.reconfigure :loose @21:42:13.08 Forearm.seek CC @21:42:14.12 Hand.close @21:42:14.72 CC.seek :open @21:42:19.33 Forearm.seek :retract @21:42:20.36 Elbow.reconfigure :tight @21:42:20.40 Elbow.seek :garage @21:42:23.33 Elbow.reconfigure :loose @21:42:23.86 Forearm.reconfigure :tight @21:42:23.91 Forearm.seek :garage @21:42:25.01 Hand.open @21:42:25.65 Forearm.reconfigure :loose @21:42:25.70 Forearm.seek :retract @21:42:26.71 PSV.dial :mixloop @21:42:26.78 PTV.dial :PRVmixing @21:42:26.80 PRV.dial CBV @21:42:26.82
CBV.dial PRV @21:42:27.02 CTV.dial :air,avoiding: :intake @21:42:27.10 Loading lyfil puck from tube 4 into Collection Clamp @21:42:27.33 SC.reconfigure :tight @21:42:27.37 SC.seek 4 @21:42:30.97 SC.configure :limp @21:42:31.18 Elbow.reconfigure :tight @21:42:31.23 Elbow.seek 4 @21:42:33.16 Forearm.reconfigure :tight @21:42:33.21 Forearm.seek 4 + 450 counts @21:42:34.38 SE.reconfigure :pick @21:42:34.44 SE.seek :beamBreak @21:42:35.88 SE.configure :default @21:42:36.05 Elbow.seek 4 @21:42:36.24 Forearm.seek 4 + 300 counts @21:42:36.46 Forearm.reconfigure :loose @21:42:36.51 Hand.close @21:42:37.10 SE.seek :down @21:42:37.30
Forearm.seek :retract @21:42:38.16 SE.stop @21:42:38.28
Elbow.seek CC @21:42:40.35 Elbow.reconfigure :loose @21:42:40.45 Forearm.reconfigure :tight @21:42:40.50 Forearm.seek CC @21:42:41.75 CC.seek :closed @21:42:46.28 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.22 @21:42:46.29 Hand.open @21:42:47.02 Forearm.reconfigure :loose @21:42:47.07 Forearm.seek :retract @21:42:48.11 CTV.dial CSV,avoiding: :intake @21:42:49.47 CSV.dial CTV @21:42:49.91 PS.seek 2.5ml @21:43:16.25 CS.seek 4.5ml @21:43:31.12 delay 8 seconds @21:43:39.14 PSV.dial :air @21:43:39.74 delay 2 seconds @21:43:41.75 PSV.dial :waste2 @21:43:42.52 PS.seek 0ml @21:44:08.84 CBV.dial between PRV and waste1 @21:44:09.17 CSV.dial :air @21:44:09.61 CS.seek 10ml @21:44:24.49 CSV.dial CTV @21:44:24.95 CS.seek 0ml @21:44:51.40 CBV.dial :waste1 @21:44:51.71 delay 5 seconds @21:44:56.74 CTV.dial :air,avoiding: :intake @21:44:58.15 Storing lyfil puck in Collection Clamp to tube 3 @21:44:58.32 SC.reconfigure :tight @21:44:58.37 SC.seek 3 @21:45:02.35 SC.configure :limp @21:45:02.64 Elbow.reconfigure :tight @21:45:02.69 Elbow.seek 3 @21:45:04.48 Forearm.reconfigure :tight @21:45:04.53 Forearm.seek 3 + 450 counts @21:45:05.55 SE.reconfigure :pick @21:45:05.61 SE.seek :beamBreak @21:45:08.23 SE.configure :default @21:45:08.39 Elbow.reconfigure :limp @21:45:08.44 Forearm.reconfigure :loose @21:45:08.48 Forearm.seek 3 @21:45:08.73 Elbow.hold @21:45:08.79 Elbow.reconfigure :tight @21:45:08.89 SE.seek 6.18 Pucks below Top Plate @21:45:09.34 Forearm.force -20 @21:45:09.38 Forearm.seek 3 + 319 counts @21:45:09.66 Forearm.reconfigure :tight @21:45:09.70 Forearm.seek 3 + 450 counts @21:45:09.89 SE.seek 7.18 Pucks below Top Plate @21:45:10.45 Forearm.reconfigure :loose @21:45:10.50 Forearm.seek :retract @21:45:11.43 Elbow.seek CC @21:45:13.07 Elbow.reconfigure :loose @21:45:13.17 Forearm.seek CC @21:45:14.16 Hand.close @21:45:14.71 CC.seek :open @21:45:19.88 Forearm.seek :retract @21:45:20.87 Elbow.reconfigure :tight @21:45:20.92 Elbow.seek 3 @21:45:22.72 Elbow.reconfigure :limp @21:45:22.77 Forearm.seek 3 @21:45:23.76 Elbow.hold @21:45:23.82 Elbow.reconfigure :tight @21:45:23.87 Hand.open @21:45:25.22 Forearm.force -20 @21:45:25.27 Forearm.seek 3 + 299 counts @21:45:25.55 Forearm.reconfigure :tight @21:45:25.60 Forearm.seek 3 + 450 counts @21:45:25.86 SE.seek :down @21:45:26.99
Forearm.reconfigure :loose @21:45:27.04 Forearm.seek :retract @21:45:28.14 SE.stop @21:45:28.18
PSV.dial :air @21:45:28.94 PS.seek 2.5ml @21:45:55.31 delay 1 second @21:45:56.33 PSV.dial :mixloop @21:45:56.78 delay 1 second @21:45:57.80 PSV.dial :air @21:45:58.26 delay 1 second @21:45:59.28 PSV.dial :mixloop @21:45:59.75 delay 1 second @21:46:00.76 PSV.dial :air @21:46:01.23 delay 1 second @21:46:02.26 PS.seek 0ml @21:46:28.62 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @21:46:28.81 Elbow.seek FlushPuck::Garage @21:46:30.64 Elbow.reconfigure :loose @21:46:30.74 Forearm.seek :garage @21:46:31.71 Hand.close @21:46:32.89 Forearm.seek :retract @21:46:33.90 Elbow.reconfigure :tight @21:46:33.95 Elbow.seek PC @21:46:35.41 Elbow.reconfigure :loose @21:46:35.51 Forearm.reconfigure :tight @21:46:35.56 Forearm.seek PC @21:46:36.82 PC.seek :closed @21:46:41.48 Processing Clamp onSpring/offSpring for 16/26 samples, on/off torque 1.64 @21:46:41.55 Hand.open @21:46:42.27 Forearm.reconfigure :loose @21:46:42.32 Forearm.seek :retract @21:46:43.33 PRV.dial :air @21:46:43.79 PSV.dial :mixloop @21:46:44.33 PS.seek 0.3ml @21:46:48.25 PSV.dial PSR @21:46:49.42 Pulling 0.4ml of PSR.air @21:46:49.43 PSR.select :air @21:46:49.45 PS.seek 0.7ml @21:46:54.34 delay 3 seconds @21:46:57.36 PSR.select :air @21:46:57.38 PS.seek 2.5ml @21:47:16.65 delay 3 seconds @21:47:19.67 PSV.dial :mixloop @21:47:20.72 PTV.dial :mixing @21:47:21.47 PBV.dial :waste2 @21:47:22.44 PS.seek 0ml @21:47:49.00 Garaging FlushPuck in Processing Clamp @21:47:49.07 Elbow.reconfigure :tight @21:47:49.12 Elbow.seek PC @21:47:49.24 Elbow.reconfigure :loose @21:47:49.76 Forearm.seek PC @21:47:50.74 Hand.close @21:47:51.34 PC.seek :open @21:47:56.08 Forearm.seek :retract @21:47:57.04 Elbow.reconfigure :tight @21:47:57.09 Elbow.seek :garage @21:47:58.42 Elbow.reconfigure :loose @21:47:58.52 Forearm.reconfigure :tight @21:47:58.57 Forearm.seek :garage @21:47:59.61 Hand.open @21:48:00.27 Forearm.reconfigure :loose @21:48:00.32 Forearm.seek :retract @21:48:01.31 Loading da puck from tube 4 into Processing Clamp @21:48:01.54 SC.reconfigure :tight @21:48:01.59 SC.seek 4 @21:48:05.19 SC.configure :limp @21:48:05.40 Elbow.reconfigure :tight @21:48:05.45 Elbow.seek 4 @21:48:07.31 Forearm.reconfigure :tight @21:48:07.36 Forearm.seek 4 + 450 counts @21:48:08.52 SE.reconfigure :pick @21:48:08.58 SE.seek :beamBreak @21:48:10.16 SE.configure :default @21:48:10.32 Elbow.seek 4 @21:48:10.51 Forearm.seek 4 + 300 counts @21:48:10.66 Forearm.reconfigure :loose @21:48:10.71 Hand.close @21:48:11.35 SE.seek :down @21:48:11.54
Forearm.seek :retract @21:48:12.50 SE.stop @21:48:12.99
Elbow.seek PC @21:48:15.55 Elbow.reconfigure :loose @21:48:15.65 Forearm.reconfigure :tight @21:48:15.70 Forearm.seek PC @21:48:16.97 PC.seek :closed @21:48:21.60 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.61 @21:48:21.61 Hand.open @21:48:22.27 Forearm.reconfigure :loose @21:48:22.32 Forearm.seek :retract @21:48:23.27 Heating Puck.from 4, :da in Processing Clamp to 28C for 2:00:00 @21:48:23.28 PH.seek 28C,5:00 @21:49:04.91 PH.hold 2:00:00,28C @21:49:04.96 delay 1:30 @21:50:35.01 PSR.select :air @21:50:35.03 PSV.dial PSR @21:50:36.09 Pulling 0.5ml of PSR.wash @21:50:36.10 PSR.select :wash @21:50:36.22 PS.seek 0.5ml @21:50:42.12 delay 5 seconds @21:50:47.14 PSR.select :air @21:50:47.26 PS.seek 2ml @21:51:03.58 PTV.dial :puck @21:51:04.34 PSV.dial PTV @21:51:05.10 PBV.dial :waste1 @21:51:06.51 PS.seek 1.5ml @21:51:12.49 delay 3 seconds @21:51:15.51 PBV.dial between PSV and waste1 @21:51:15.86 PS.seek 0ml @21:51:32.03 delay 5:00 @21:56:32.05 recoverPSRreagent08 @21:56:32.06 PTV.dial :puck @21:56:32.07 PBV.dial :waste1 @21:56:32.43 delay 30 seconds @21:57:02.46 PBV.dial between PSV and waste1 @21:57:02.81 PSR.select :air @21:57:02.83 PSV.dial PSR @21:57:03.60 PS.reconfigure :fast @21:57:03.65 PS.seek 2.5ml @21:57:27.55 PSV.dial PTV @21:57:28.32 delay 5 seconds @21:57:33.33 PSV.dial PSR @21:57:34.10 delay 5 seconds @21:57:39.12 PSV.dial PTV @21:57:40.00 delay 5 seconds @21:57:45.02 PSV.dial :air @21:57:45.80 PS.seek 0ml @21:58:09.14 PTV.dial :PRVmixing,via: PRV @21:58:10.49 PSV.dial :mixloop @21:58:10.97 PRV.dial :ab1 @21:58:11.73 PS.reconfigure :slow @21:58:11.77 PS.seek 0.15ml @21:58:15.61 delay 2 seconds @21:58:17.63 CBV.dial PRV @21:58:17.65
PRV.dial CBV @21:58:18.66 PS.seek 0.35ml @21:58:23.33 delay 2 seconds @21:58:25.35 CBV.dial between PRV and PSV @21:58:25.67 PRV.dial :air @21:58:26.13 PS.seek 0.9ml @21:58:37.92 PRV.dial :waste2 @21:58:38.68 PS.seek 0.5ml @21:58:47.33 addPMLReagent08 @21:58:47.34 PSV.dial :air @21:58:47.80 PS.reconfigure :default @21:58:47.85 PS.seek 2ml @21:59:04.05 PSV.dial :PML @21:59:04.52 PTV.dial :mixing @21:59:05.28 PBV.dial :waste1 @21:59:05.72 PS.seek 1.85ml @21:59:08.08 PBV.dial between PSV and waste1 @21:59:08.46 PS.seek 0ml @21:59:28.11 delay 2 seconds @21:59:30.13 Beginning samplePassC @21:59:30.14 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @21:59:30.32 Elbow.reconfigure :tight @21:59:30.37 Elbow.seek FlushPuck::Garage @21:59:31.73 Elbow.reconfigure :loose @21:59:31.83 Forearm.seek :garage @21:59:32.83 Hand.close @21:59:33.58 Forearm.seek :retract @21:59:34.59 Elbow.reconfigure :tight @21:59:34.64 Elbow.seek CC @21:59:37.52 Elbow.reconfigure :loose @21:59:37.62 Forearm.reconfigure :tight @21:59:37.67 Forearm.seek CC @21:59:38.94 CC.seek :closed @21:59:43.46 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.49 @21:59:43.53 Hand.open @21:59:44.26 Forearm.reconfigure :loose @21:59:44.31 Forearm.seek :retract @21:59:45.26 PSV.dial PSR @21:59:46.33 Pulling 1.5ml of PSR.flush @21:59:46.34 PSR.select :flush @21:59:46.46 PS.seek 1.5ml @22:00:02.58 delay 5 seconds @22:00:07.60 PSV.dial :waste1 @22:00:08.07 PS.seek 0ml @22:00:24.23 PSV.dial PSR @22:00:24.70 PSR.select :air @22:00:24.82 PS.seek 2.5ml @22:00:51.21 PRV.dial CBV,avoiding: :ab2 @22:00:51.23
CBV.dial PRV @22:00:52.86 PSV.dial :mixloop @22:00:54.03 CTV.dial :air,avoiding: :intake @22:00:54.11 PTV.dial :PRVmixing @22:00:54.90 PS.seek 0ml @22:01:21.20 PSV.dial :air @22:01:21.69 PS.seek 2.5ml @22:01:48.08 PSV.dial :mixloop @22:01:48.57 PS.seek 0ml @22:02:14.89 PSV.dial :air @22:02:15.49 PS.seek 2.5ml @22:02:41.89 PSV.dial :mixloop @22:02:42.35 PS.seek 0ml @22:03:08.89 Garaging FlushPuck in Collection Clamp @22:03:08.96 Elbow.reconfigure :tight @22:03:09.01 Elbow.seek CC @22:03:09.26 Elbow.reconfigure :loose @22:03:09.36 Forearm.seek CC @22:03:10.40 Hand.close @22:03:11.46 CC.seek :open @22:03:16.19 Forearm.seek :retract @22:03:17.23 Elbow.reconfigure :tight @22:03:17.28 Elbow.seek :garage @22:03:20.24 Elbow.reconfigure :loose @22:03:20.34 Forearm.reconfigure :tight @22:03:20.39 Forearm.seek :garage @22:03:21.38 Hand.open @22:03:22.01 Forearm.reconfigure :loose @22:03:22.06 Forearm.seek :retract @22:03:23.00 delayUntil "22:07:30.12" @22:03:23.03 Whole Cell Archive sampling 1000ml @22:03:23.04 Loading wcr puck from tube 4 into Collection Clamp @22:03:23.28 SC.reconfigure :tight @22:03:23.33 SC.seek 4 @22:03:23.40 SC.configure :limp @22:03:23.62 Elbow.reconfigure :tight @22:03:23.67 Elbow.seek 4 @22:03:25.69 Forearm.reconfigure :tight @22:03:25.75 Forearm.seek 4 + 450 counts @22:03:26.76 SE.reconfigure :pick @22:03:26.82 SE.seek :beamBreak @22:03:28.46 SE.configure :default @22:03:28.63 Elbow.seek 4 @22:03:29.13 Forearm.seek 4 + 300 counts @22:03:29.32 Forearm.reconfigure :loose @22:03:29.37 Hand.close @22:03:29.96 SE.seek :down @22:03:30.61 Forearm.seek :retract @22:03:31.62 SE.stop @22:03:31.68 Elbow.seek CC @22:03:33.38 Elbow.reconfigure :loose @22:03:33.49 Forearm.reconfigure :tight @22:03:33.54 Forearm.seek CC @22:03:34.85 CC.seek :closed @22:03:39.40 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.43 @22:03:39.41 Hand.open @22:03:40.14 Forearm.reconfigure :loose @22:03:40.19 Forearm.seek :retract @22:03:41.17 collectSample 1000ml w/bubble point of 35psi @22:03:41.18 Priming 7ml @22:03:41.19 Intake.select :close @22:03:41.21 Exhaust.select :close @22:03:41.22 SS.hold @22:03:41.43 Calibrating pressure sensors @22:03:41.44 CTV.dial :air @22:03:41.51 SSV.dial :spit,avoiding: :exhaust @22:03:41.60 SS.reconfigure :fast @22:03:41.66 SS.seek 5ml @22:03:44.55 SSV.dial CBV @22:03:44.57 CBV.dial SSV @22:03:45.94 delay 3.8 seconds @22:03:49.88 Sensor limits: Intake max 104.0psia; Outlet max 96.4psia @22:03:49.90 SSV.dial :spit @22:03:51.23 SS.seek 0ml @22:03:53.91 SSV.dial CBV @22:03:55.70 CTV.dial :intake @22:03:56.48 SSV.dial CBV @22:03:56.51 CBV.dial SSV @22:03:56.59 Allowing 40s for 7.0ml of remaining 7.0ml @22:03:56.60 Intake.select :open @22:03:58.77 Intake @14.2psia, vacuum from 8.7 to 11.7psi @22:03:58.79 SS.reconfigure :inhaling 2.20ml/s @22:03:58.84 SS.seek 7ml @22:04:05.59 Stroke completed -- waiting for pressure to equalize ... @22:04:05.61 SS.reconfigure :exhale @22:04:05.66 SS.hold @22:04:24.03 Intake.select :close @22:04:24.64 SSV.dial :exhaust @22:04:25.41 Exhaust.select :open @22:04:25.53 SS.seek 0ml @22:04:29.66 Exhaust.select :close @22:04:29.77 Sampling 1000.0ml @22:04:29.78 Intake.select :close @22:04:29.80 Exhaust.select :close @22:04:29.81 SS.reconfigure :default @22:04:29.85 SS.hold @22:04:30.06 CTV.dial :intake @22:04:30.23 SSV.dial CBV @22:04:30.25 CBV.dial SSV @22:04:31.05 Allowing 130s for 25.0ml of remaining 1000.0ml @22:04:31.06 Intake.select :open @22:04:33.23 Intake @15.3psia, vacuum from 9.8 to 12.8psi @22:04:33.25 SS.reconfigure :inhaling 2.20ml/s @22:04:33.30 SS.seek 25ml @22:04:41.18 Vacuum too high -- waiting up to 40 seconds @22:04:41.20 SS.reconfigure :exhale @22:04:41.26 SS.hold @22:04:50.65 Intake @17.9psia, vacuum from 10.0 to 13.0psi @22:04:50.67 SS.reconfigure :inhaling 1.76ml/s @22:04:50.72 SS.seek 25ml @22:04:54.15 Vacuum too high -- waiting up to 40 seconds @22:04:54.17 SS.reconfigure :exhale @22:04:54.22 SS.hold @22:04:56.92 Intake @17.2psia, vacuum from 10.0 to 13.0psi @22:04:56.94 SS.reconfigure :inhaling 1.41ml/s @22:04:56.99 SS.seek 25ml @22:04:59.99 Vacuum too high -- waiting up to 40 seconds @22:05:00.01 SS.reconfigure :exhale @22:05:00.07 SS.hold @22:05:02.69 Intake @17.5psia, vacuum from 10.0 to 13.0psi @22:05:02.71 SS.reconfigure :inhaling 1.13ml/s @22:05:02.76 SS.seek 25ml @22:05:06.25 Vacuum too high -- waiting up to 40 seconds @22:05:06.26 SS.reconfigure :exhale @22:05:06.32 SS.hold @22:05:08.49 Intake @18.9psia, vacuum from 10.0 to 13.0psi @22:05:08.51 SS.reconfigure :inhaling 0.91ml/s @22:05:08.56 SS.seek 25ml @22:05:11.69 Vacuum too high -- waiting up to 40 seconds @22:05:11.70 SS.reconfigure :exhale @22:05:11.76 SS.hold @22:05:13.50 Intake @17.2psia, vacuum from 10.0 to 13.0psi @22:05:13.52 SS.reconfigure :inhaling 0.73ml/s @22:05:13.57 SS.seek 25ml @22:05:15.76 Stroke completed -- waiting for pressure to equalize ... @22:05:15.78 SS.reconfigure :exhale @22:05:15.83 SS.hold @22:05:42.41 Intake.select :close @22:05:42.57 SSV.dial :exhaust @22:05:43.33 Exhaust.select :open @22:05:43.45 SS.seek 0ml @22:05:52.28 Exhaust.select :close @22:05:52.39 SSV.dial CBV @22:05:52.41 CBV.dial SSV @22:05:53.20 Allowing 130s for 25.0ml of remaining 975.0ml @22:05:53.22 Intake.select :open @22:05:55.39 Intake @15.7psia, vacuum from 10.0 to 13.0psi @22:05:55.41 SS.reconfigure :inhaling 0.73ml/s @22:05:55.46 SS.seek 25ml @22:06:32.15 Stroke completed -- waiting for pressure to equalize ... @22:06:32.17 SS.reconfigure :exhale @22:06:32.22 SS.hold @22:07:03.32 Intake.select :close @22:07:03.48 SSV.dial :exhaust @22:07:04.25 Exhaust.select :open @22:07:04.37 SS.seek 0ml @22:07:13.40 Exhaust.select :close @22:07:13.51 SSV.dial CBV @22:07:13.55 CBV.dial SSV @22:07:14.31 Allowing 130s for 25.0ml of remaining 950.0ml @22:07:14.33 Intake.select :open @22:07:16.49 Intake @15.7psia, vacuum from 10.0 to 13.0psi @22:07:16.51 SS.reconfigure :inhaling 0.73ml/s @22:07:16.56 SS.seek 25ml @22:07:30.12 recoverPRVreagent08 @22:07:30.14 PTV.dial :mixing @22:07:30.90 PBV.dial :waste1 @22:07:31.27 delay 30 seconds @22:07:52.79 Stroke completed -- waiting for pressure to equalize ... @22:07:52.81 SS.reconfigure :exhale @22:07:52.85 SS.hold @22:08:01.29 PBV.dial between PSV and waste1 @22:08:01.65 PSV.dial :air @22:08:02.26 PS.reconfigure :fast @22:08:02.31 PS.seek 2.5ml @22:08:21.89 Intake.select :close @22:08:22.05 SSV.dial :exhaust @22:08:22.81 Exhaust.select :open @22:08:22.93 SS.seek 0ml @22:08:26.69 PSV.dial :mixloop @22:08:27.15 delay 2 seconds @22:08:29.17 PSV.dial :air @22:08:29.63 delay 2 seconds @22:08:31.65 PSV.dial :mixloop @22:08:31.72 Exhaust.select :close @22:08:31.83 SSV.dial CBV @22:08:31.85 CBV.dial SSV @22:08:32.13 delay 2 seconds @22:08:32.62 Allowing 130s for 25.0ml of remaining 925.0ml @22:08:32.64 Intake.select :open @22:08:34.16 PSV.dial :air @22:08:34.76 PS.seek 0ml @22:08:34.87 Intake @15.8psia, vacuum from 10.0 to 13.0psi @22:08:34.89 SS.reconfigure :inhaling 0.73ml/s @22:08:34.94 SS.seek 25ml @22:08:58.23 washCycleDA #1 of 2 @22:08:58.24 PSR.select :air @22:08:58.25 PSV.dial PSR @22:08:59.62 Pulling 0.5ml of PSR.wash @22:08:59.63 PSR.select :wash @22:08:59.75 PS.reconfigure :default @22:08:59.79 PS.seek 0.5ml @22:09:05.79 delay 5 seconds @22:09:10.81 PSR.select :air @22:09:10.93 PS.seek 2ml @22:09:11.04 Stroke completed -- waiting for pressure to equalize ... @22:09:11.06 SS.reconfigure :exhale @22:09:11.10 SS.hold @22:09:27.03 PTV.dial :puck @22:09:27.79 PSV.dial PTV @22:09:28.57 PBV.dial :waste1 @22:09:29.00 PS.seek 1.5ml @22:09:35.00 delay 3 seconds @22:09:37.65 Intake.select :close @22:09:37.81 SSV.dial :exhaust @22:09:38.02 PBV.dial between PSV and waste1 @22:09:38.40 PS.seek 0ml @22:09:38.60 Exhaust.select :open @22:09:38.72 SS.seek 0ml @22:09:47.78 Exhaust.select :close @22:09:47.89 SSV.dial CBV @22:09:47.92 CBV.dial SSV @22:09:48.68 Allowing 130s for 25.0ml of remaining 900.0ml @22:09:48.70 Intake.select :open @22:09:50.87 Intake @15.7psia, vacuum from 10.0 to 13.0psi @22:09:50.89 SS.reconfigure :inhaling 0.73ml/s @22:09:50.94 SS.seek 25ml @22:09:54.57 delay 2:00 @22:10:26.68 Stroke completed -- waiting for pressure to equalize ... @22:10:26.69 SS.reconfigure :exhale @22:10:26.74 SS.hold @22:10:51.62 Intake.select :close @22:10:51.78 SSV.dial :exhaust @22:10:52.54 Exhaust.select :open @22:10:52.66 SS.seek 0ml @22:11:02.11 Exhaust.select :close @22:11:02.30 SSV.dial CBV @22:11:02.32 CBV.dial SSV @22:11:03.09 Allowing 130s for 25.0ml of remaining 875.0ml @22:11:03.11 Intake.select :open @22:11:05.27 Intake @15.7psia, vacuum from 10.0 to 13.0psi @22:11:05.29 SS.reconfigure :inhaling 0.73ml/s @22:11:05.34 SS.seek 25ml @22:11:41.15 Stroke completed -- waiting for pressure to equalize ... @22:11:41.16 SS.reconfigure :exhale @22:11:41.21 SS.hold @22:11:54.59 recoverPSRreagent08 @22:11:54.60 PTV.dial :puck @22:11:54.83 PBV.dial :waste1 @22:11:55.21 delay 30 seconds @22:12:07.67 Intake.select :close @22:12:07.83 SSV.dial :exhaust @22:12:08.60 Exhaust.select :open @22:12:08.71 SS.seek 0ml @22:12:17.68 Exhaust.select :close @22:12:17.80 SSV.dial CBV @22:12:17.84 CBV.dial SSV @22:12:18.59 Allowing 130s for 25.0ml of remaining 850.0ml @22:12:18.60 Intake.select :open @22:12:20.77 Intake @15.6psia, vacuum from 10.0 to 13.0psi @22:12:20.79 SS.reconfigure :inhaling 0.73ml/s @22:12:20.84 SS.seek 25ml @22:12:25.22 PBV.dial between PSV and waste1 @22:12:25.60 PSR.select :air @22:12:25.62 PSV.dial PSR @22:12:26.38 PS.reconfigure :fast @22:12:26.43 PS.seek 2.5ml @22:12:50.69 PSV.dial PTV @22:12:51.46 delay 5 seconds @22:12:56.48 PSV.dial PSR @22:12:56.97 Stroke completed -- waiting for pressure to equalize ... @22:12:56.99 SS.reconfigure :exhale @22:12:57.04 SS.hold @22:12:57.24 delay 5 seconds @22:13:02.32 PSV.dial PTV @22:13:03.13 delay 5 seconds @22:13:08.16 PSV.dial :air @22:13:09.05 PS.seek 0ml @22:13:21.98 Intake.select :close @22:13:22.14 SSV.dial :exhaust @22:13:22.91 Exhaust.select :open @22:13:23.03 SS.seek 0ml @22:13:32.23 Exhaust.select :close @22:13:32.34 SSV.dial CBV @22:13:32.37 CBV.dial SSV @22:13:32.75 washCycleDA #2 of 2 @22:13:32.77 PSR.select :air @22:13:32.78 PSV.dial PSR @22:13:33.13 Allowing 130s for 25.0ml of remaining 825.0ml @22:13:33.15 Intake.select :open @22:13:34.18 Pulling 0.5ml of PSR.wash @22:13:34.21 PSR.select :wash @22:13:34.34 PS.reconfigure :default @22:13:34.39 PS.seek 0.5ml @22:13:35.32 Intake @15.7psia, vacuum from 10.0 to 13.0psi @22:13:35.34 SS.reconfigure :inhaling 0.73ml/s @22:13:35.39 SS.seek 25ml @22:13:40.35 delay 5 seconds @22:13:45.37 PSR.select :air @22:13:45.49 PS.seek 2ml @22:14:01.66 PTV.dial :puck @22:14:01.73 PSV.dial PTV @22:14:02.97 PBV.dial :waste1 @22:14:03.41 PS.seek 1.5ml @22:14:09.34 delay 3 seconds @22:14:10.79 Stroke completed -- waiting for pressure to equalize ... @22:14:10.80 SS.reconfigure :exhale @22:14:10.85 SS.hold @22:14:12.41 PBV.dial between PSV and waste1 @22:14:12.91 PS.seek 0ml @22:14:29.00 delay 2:00 @22:14:39.45 Intake.select :close @22:14:39.61 SSV.dial :exhaust @22:14:40.38 Exhaust.select :open @22:14:40.50 SS.seek 0ml @22:14:49.33 Exhaust.select :close @22:14:49.44 SSV.dial CBV @22:14:49.48 CBV.dial SSV @22:14:50.26 Allowing 130s for 25.0ml of remaining 800.0ml @22:14:50.27 Intake.select :open @22:14:52.44 Intake @15.7psia, vacuum from 10.0 to 13.0psi @22:14:52.46 SS.reconfigure :inhaling 0.73ml/s @22:14:52.51 SS.seek 25ml @22:15:25.73 Vacuum too high -- waiting up to 40 seconds @22:15:25.75 SS.reconfigure :exhale @22:15:25.81 SS.hold @22:15:40.84 Intake @17.4psia, vacuum from 10.0 to 13.0psi @22:15:40.86 SS.reconfigure :inhaling 0.59ml/s @22:15:40.90 SS.seek 25ml @22:15:44.72 Stroke completed -- waiting for pressure to equalize ... @22:15:44.73 SS.reconfigure :exhale @22:15:44.78 SS.hold @22:15:57.48 Intake.select :close @22:15:57.64 SSV.dial :exhaust @22:15:58.40 Exhaust.select :open @22:15:58.97 SS.seek 0ml @22:16:08.00 Exhaust.select :close @22:16:08.11 SSV.dial CBV @22:16:08.14 CBV.dial SSV @22:16:08.91 Allowing 130s for 25.0ml of remaining 775.0ml @22:16:08.93 Intake.select :open @22:16:11.09 Intake @15.7psia, vacuum from 10.0 to 13.0psi @22:16:11.11 SS.reconfigure :inhaling 0.59ml/s @22:16:11.16 SS.seek 25ml @22:16:29.02 recoverPSRreagent08 @22:16:29.03 PTV.dial :puck @22:16:29.11 PBV.dial :waste1 @22:16:29.48 delay 30 seconds @22:16:55.57 Stroke completed -- waiting for pressure to equalize ... @22:16:55.58 SS.reconfigure :exhale @22:16:55.63 SS.hold @22:16:59.50 PBV.dial between PSV and waste1 @22:16:59.87 PSR.select :air @22:16:59.89 PSV.dial PSR @22:17:00.67 PS.reconfigure :fast @22:17:00.72 PS.seek 2.5ml @22:17:22.18 Intake.select :close @22:17:22.34 SSV.dial :exhaust @22:17:23.11 Exhaust.select :open @22:17:23.22 SS.seek 0ml @22:17:24.93 PSV.dial PTV @22:17:25.70 delay 5 seconds @22:17:30.72 PSV.dial PSR @22:17:31.48 delay 5 seconds @22:17:32.24 Exhaust.select :close @22:17:32.35 SSV.dial CBV @22:17:32.37 CBV.dial SSV @22:17:33.15 Allowing 130s for 25.0ml of remaining 750.0ml @22:17:33.16 Intake.select :open @22:17:35.33 Intake @15.6psia, vacuum from 10.0 to 13.0psi @22:17:35.35 SS.reconfigure :inhaling 0.59ml/s @22:17:35.40 SS.seek 25ml @22:17:36.50 PSV.dial PTV @22:17:37.26 delay 5 seconds @22:17:42.28 PSV.dial :air @22:17:43.18 PS.seek 0ml @22:18:06.68 getPMLReagent08 @22:18:06.70 PRV.dial :air @22:18:07.14 delay 5 seconds @22:18:12.16 PTV.dial :PRVmixing @22:18:13.50 delay 5 seconds @22:18:18.52 PRV.dial :ab2 @22:18:18.97 PTV.dial :PRVmixing @22:18:19.04 PSV.dial :mixloop @22:18:19.50 PS.reconfigure :default @22:18:19.55 PS.seek 0.3ml @22:18:19.78 Stroke completed -- waiting for pressure to equalize ... @22:18:19.79 SS.reconfigure :exhale @22:18:19.84 SS.hold @22:18:23.46 delay 2 seconds @22:18:25.48 PRV.dial :air @22:18:26.01 PS.seek 0.55ml @22:18:29.40 addPMLReagent08 @22:18:29.41 PSV.dial :air @22:18:29.89 PS.seek 2ml @22:18:45.59 PSV.dial :PML @22:18:46.07 PTV.dial :mixing @22:18:46.85 PBV.dial :waste1 @22:18:47.29 PS.seek 1.85ml @22:18:49.78 PBV.dial between PSV and waste1 @22:18:50.14 PS.seek 0ml @22:18:53.24 Intake.select :close @22:18:53.40 SSV.dial :exhaust @22:18:54.17 Exhaust.select :open @22:18:54.29 SS.seek 0ml @22:19:03.30 Exhaust.select :close @22:19:03.41 SSV.dial CBV @22:19:03.46 CBV.dial SSV @22:19:04.21 Allowing 130s for 25.0ml of remaining 725.0ml @22:19:04.22 Intake.select :open @22:19:06.39 Intake @15.8psia, vacuum from 10.0 to 13.0psi @22:19:06.41 SS.reconfigure :inhaling 0.59ml/s @22:19:06.46 SS.seek 25ml @22:19:09.84 delay 2 seconds @22:19:11.86 delay 8:00 @22:19:37.42 Vacuum too high -- waiting up to 40 seconds @22:19:37.43 SS.reconfigure :exhale @22:19:37.49 SS.hold @22:19:52.47 Intake @17.0psia, vacuum from 10.0 to 13.0psi @22:19:52.49 SS.reconfigure :inhaling 0.47ml/s @22:19:52.54 SS.seek 25ml @22:20:09.23 Stroke completed -- waiting for pressure to equalize ... @22:20:09.25 SS.reconfigure :exhale @22:20:09.29 SS.hold @22:20:29.79 Intake.select :close @22:20:29.95 SSV.dial :exhaust @22:20:30.73 Exhaust.select :open @22:20:30.85 SS.seek 0ml @22:20:39.95 Exhaust.select :close @22:20:40.06 SSV.dial CBV @22:20:40.11 CBV.dial SSV @22:20:40.85 Allowing 130s for 25.0ml of remaining 700.0ml @22:20:40.86 Intake.select :open @22:20:43.03 Intake @15.6psia, vacuum from 10.0 to 13.0psi @22:20:43.05 SS.reconfigure :inhaling 0.47ml/s @22:20:43.10 SS.seek 25ml @22:21:38.15 Stroke completed -- waiting for pressure to equalize ... @22:21:38.17 SS.reconfigure :exhale @22:21:38.21 SS.hold @22:22:13.32 Intake.select :close @22:22:13.48 SSV.dial :exhaust @22:22:14.25 Exhaust.select :open @22:22:14.37 SS.seek 0ml @22:22:23.20 Exhaust.select :close @22:22:23.32 SSV.dial CBV @22:22:23.34 CBV.dial SSV @22:22:24.12 Allowing 130s for 25.0ml of remaining 675.0ml @22:22:24.14 Intake.select :open @22:22:26.31 Intake @15.7psia, vacuum from 10.0 to 13.0psi @22:22:26.33 SS.reconfigure :inhaling 0.47ml/s @22:22:26.38 SS.seek 25ml @22:23:21.94 Vacuum too high -- waiting up to 40 seconds @22:23:21.96 SS.reconfigure :exhale @22:23:22.01 SS.hold @22:23:44.75 Intake @16.4psia, vacuum from 10.0 to 13.0psi @22:23:44.77 SS.reconfigure :inhaling 0.38ml/s @22:23:44.81 SS.seek 25ml @22:23:44.88 Stroke completed -- waiting for pressure to equalize ... @22:23:44.89 SS.reconfigure :exhale @22:23:44.94 SS.hold @22:24:03.35 Intake.select :close @22:24:03.51 SSV.dial :exhaust @22:24:04.28 Exhaust.select :open @22:24:04.40 SS.seek 0ml @22:24:13.46 Exhaust.select :close @22:24:13.57 SSV.dial CBV @22:24:13.60 CBV.dial SSV @22:24:14.39 Allowing 130s for 25.0ml of remaining 650.0ml @22:24:14.40 Intake.select :open @22:24:16.57 Intake @15.6psia, vacuum from 10.0 to 13.0psi @22:24:16.59 SS.reconfigure :inhaling 0.38ml/s @22:24:16.64 SS.seek 25ml @22:25:24.96 Stroke completed -- waiting for pressure to equalize ... @22:25:24.97 SS.reconfigure :exhale @22:25:25.02 SS.hold @22:26:05.21 SS.reconfigure :default @22:26:05.27 SS.hold @22:26:05.80 Intake.select :close @22:26:05.96 ShallowSampler::Clogged in WCR -- Intake @15psia & Outlet @14psia won't equalize after 375.0ml @22:26:05.98 SSV.dial :exhaust @22:26:06.76 Exhaust.select :open @22:26:06.88 SS.reconfigure :exhale @22:26:06.93 SS.seek 0ml @22:26:15.77 Exhaust.select :close @22:26:15.88 Sampled 375.0ml @22:26:15.90 Drying puck before fixing for 5:00 @22:26:15.91 CBV.dial :waste2b @22:26:16.68 delay 1:00 @22:27:11.88 recoverPRVreagent08 @22:27:11.89 PTV.dial :mixing @22:27:11.97 PBV.dial :waste1 @22:27:12.35 delay 30 seconds @22:27:16.70 CSV.dial :air @22:27:17.17 CS.reconfigure :fast @22:27:17.23 CS.seek 10ml @22:27:41.90 Pumping wcr puck from tube 4 up to 35psi... @22:27:41.92 CBV.dial SSV @22:27:42.36 PBV.dial between PSV and waste1 @22:27:42.74 PSV.dial :air @22:27:43.21 CBV.dial between CSV and waste2b @22:27:43.29 PS.reconfigure :fast @22:27:43.34 PS.seek 2.5ml @22:27:44.22 CSV.dial between air and CTV @22:27:44.54 CS.seek 7.3ml @22:27:51.53 CTV.dial CSV,avoiding: :air @22:27:51.58 CSV.dial CTV @22:27:52.35 CS.reconfigure :pressurize @22:27:52.41 CS.seek 0ml @22:28:07.45 PSV.dial :mixloop @22:28:07.94 delay 2 seconds @22:28:08.60 CBV.dial :waste2b @22:28:08.95 CS.reconfigure :default @22:28:09.00 CS.seek 0ml @22:28:09.96 PSV.dial :air @22:28:10.42 delay 2 seconds @22:28:12.44 PSV.dial :mixloop @22:28:12.90 delay 1:00 @22:28:12.94 delay 2 seconds @22:28:14.96 PSV.dial :air @22:28:15.43 PS.seek 0ml @22:28:38.99 washCycleDA #1 of 3 @22:28:39.00 PSR.select :air @22:28:39.02 PSV.dial PSR @22:28:40.36 Pulling 0.5ml of PSR.wash @22:28:40.37 PSR.select :wash @22:28:40.93 PS.reconfigure :default @22:28:40.99 PS.seek 0.5ml @22:28:46.86 delay 5 seconds @22:28:51.88 PSR.select :air @22:28:52.00 PS.seek 2ml @22:29:08.23 PTV.dial :puck @22:29:08.99 PSV.dial PTV @22:29:09.86 PBV.dial :waste1 @22:29:10.30 PS.seek 1.5ml @22:29:12.93 CTV.dial :air,avoiding: :intake @22:29:14.27 Fix #1 of 2 (with RNAlater) for 2:30 @22:29:14.29 CSV.dial CSR @22:29:14.72 Pulling 2ml of CSR.RNAlater @22:29:14.73 CSR.select :RNAlater @22:29:14.85 CS.seek 2ml @22:29:16.23 delay 3 seconds @22:29:19.25 PBV.dial between PSV and waste1 @22:29:19.63 PS.seek 0ml @22:29:20.86 delay 5 seconds @22:29:25.88 CSR.select :air @22:29:26.00 CS.reconfigure :fast @22:29:26.05 CS.seek 10ml @22:29:35.77 delay 2:00 @22:29:45.87 Pumping wcr puck from tube 4 up to 35psi... @22:29:45.88 CBV.dial SSV @22:29:47.15 CBV.dial between CSV and waste2b @22:29:48.14 CSV.dial between air and CTV @22:29:48.87 CS.seek 7.25ml @22:29:56.09 CTV.dial CSV,avoiding: :air @22:29:56.11 CSV.dial CTV @22:29:57.49 CS.reconfigure :pressurize @22:29:57.55 CS.seek 0ml @22:30:11.67 delay 2:30 @22:31:35.79 recoverPSRreagent08 @22:31:35.80 PTV.dial :puck @22:31:35.87 PBV.dial :waste1 @22:31:36.25 delay 30 seconds @22:32:06.28 PBV.dial between PSV and waste1 @22:32:06.65 PSR.select :air @22:32:06.67 PSV.dial PSR @22:32:07.43 PS.reconfigure :fast @22:32:07.47 PS.seek 2.5ml @22:32:31.54 PSV.dial PTV @22:32:32.44 delay 5 seconds @22:32:37.46 PSV.dial PSR @22:32:38.20 delay 5 seconds @22:32:41.69 CBV.dial :waste2b @22:32:42.04 CS.reconfigure :default @22:32:42.55 CS.seek 0ml @22:32:43.22 PSV.dial PTV @22:32:43.98 delay 5 seconds @22:32:48.17 delay 1:00 @22:32:49.00 PSV.dial :air @22:32:49.89 PS.seek 0ml @22:33:13.45 washCycleDA #2 of 3 @22:33:13.46 PSR.select :air @22:33:13.48 PSV.dial PSR @22:33:14.82 Pulling 0.5ml of PSR.wash @22:33:14.84 PSR.select :wash @22:33:14.96 PS.reconfigure :default @22:33:15.00 PS.seek 0.5ml @22:33:20.89 delay 5 seconds @22:33:25.91 PSR.select :air @22:33:26.03 PS.seek 2ml @22:33:42.26 PTV.dial :puck @22:33:42.34 PSV.dial PTV @22:33:43.10 PBV.dial :waste1 @22:33:43.54 PS.seek 1.5ml @22:33:48.19 CTV.dial :air,avoiding: :intake @22:33:49.54 Fix #2 of 2 (with RNAlater) for 2:30 @22:33:49.56 CSV.dial CSR @22:33:49.62 delay 3 seconds @22:33:50.12 Pulling 2ml of CSR.RNAlater @22:33:50.14 CSR.select :RNAlater @22:33:50.25 CS.seek 2ml @22:33:52.64 PBV.dial between PSV and waste1 @22:33:53.03 PS.seek 0ml @22:33:56.17 delay 5 seconds @22:34:01.19 CSR.select :air @22:34:01.31 CS.reconfigure :fast @22:34:01.37 CS.seek 10ml @22:34:09.23 delay 2:00 @22:34:21.21 Pumping wcr puck from tube 4 up to 35psi... @22:34:21.22 CBV.dial SSV @22:34:22.49 CBV.dial between CSV and waste2b @22:34:23.48 CSV.dial between air and CTV @22:34:24.07 CS.seek 7.201ml @22:34:31.40 CTV.dial CSV,avoiding: :air @22:34:31.42 CSV.dial CTV @22:34:32.80 CS.reconfigure :pressurize @22:34:32.85 CS.seek 0ml @22:34:46.79 delay 2:30 @22:36:09.26 recoverPSRreagent08 @22:36:09.27 PTV.dial :puck @22:36:09.35 PBV.dial :waste1 @22:36:09.73 delay 30 seconds @22:36:39.75 PBV.dial between PSV and waste1 @22:36:40.60 PSR.select :air @22:36:40.62 PSV.dial PSR @22:36:41.39 PS.reconfigure :fast @22:36:41.44 PS.seek 2.5ml @22:37:05.60 PSV.dial PTV @22:37:06.37 delay 5 seconds @22:37:11.39 PSV.dial PSR @22:37:12.15 delay 5 seconds @22:37:16.81 CBV.dial :waste2b @22:37:17.17 CS.reconfigure :default @22:37:17.23 CS.seek 0ml @22:37:17.27 PSV.dial PTV @22:37:18.06 delay 5 seconds @22:37:22.89 delay 1:00 @22:37:23.07 PSV.dial :air @22:37:23.84 PS.seek 0ml @22:37:47.38 washCycleDA #3 of 3 @22:37:47.39 PSR.select :air @22:37:47.41 PSV.dial PSR @22:37:48.74 Pulling 0.5ml of PSR.wash @22:37:48.75 PSR.select :wash @22:37:48.87 PS.reconfigure :default @22:37:48.92 PS.seek 0.5ml @22:37:54.82 delay 5 seconds @22:37:59.84 PSR.select :air @22:37:59.96 PS.seek 2ml @22:38:16.16 PTV.dial :puck @22:38:16.24 PSV.dial PTV @22:38:16.99 PBV.dial :waste1 @22:38:17.43 PS.seek 1.5ml @22:38:22.91 CTV.dial :air,avoiding: :intake @22:38:23.44 delay 3 seconds @22:38:26.46 PBV.dial between PSV and waste1 @22:38:26.83 PS.seek 0ml @22:38:42.91 delay 2:00 @22:40:42.94 recoverPSRreagent08 @22:40:42.95 PTV.dial :puck @22:40:43.02 PBV.dial :waste1 @22:40:43.40 delay 30 seconds @22:41:13.42 PBV.dial between PSV and waste1 @22:41:13.80 PSR.select :air @22:41:13.82 PSV.dial PSR @22:41:14.59 PS.reconfigure :fast @22:41:14.64 PS.seek 2.5ml @22:41:38.78 PSV.dial PTV @22:41:39.68 delay 5 seconds @22:41:44.70 PSV.dial PSR @22:41:45.44 delay 5 seconds @22:41:50.46 PSV.dial PTV @22:41:51.22 delay 5 seconds @22:41:56.24 PSV.dial :air @22:41:57.01 PS.seek 0ml @22:42:20.53 Gate.power :camera,:ON @22:42:20.55 Mixing DA Substrates @22:42:20.56 PSR.select :air @22:42:20.57 PSV.dial PSR @22:42:22.01 Pulling 0.25ml of PSR.sub1 @22:42:22.03 PSR.select :sub1 @22:42:22.22 PS.reconfigure :default @22:42:22.33 PS.seek 0.25ml @22:42:25.79 delay 5 seconds @22:42:30.81 Pulling 0.25ml of PSR.sub2 @22:42:30.83 PSR.select :sub2 @22:42:31.36 PS.seek 0.5ml @22:42:34.78 delay 5 seconds @22:42:39.80 PSR.select :air @22:42:39.92 PS.seek 2ml @22:42:56.06 PTV.dial :puck @22:42:56.14 PSV.dial PTV @22:42:56.89 PBV.dial :waste1 @22:42:57.33 PS.seek 1.5ml @22:43:03.28 delay 3 seconds @22:43:06.30 PBV.dial between PSV and waste1 @22:43:06.68 PS.seek 0ml @22:43:22.88 delay 10 seconds @22:43:32.90 recoverPSRreagent08 @22:43:32.91 PTV.dial :puck @22:43:32.99 PBV.dial :waste1 @22:43:33.38 delay 30 seconds @22:44:03.40 PBV.dial between PSV and waste1 @22:44:03.78 PSR.select :air @22:44:03.80 PSV.dial PSR @22:44:04.58 PS.reconfigure :fast @22:44:04.63 PS.seek 2.5ml @22:44:29.06 PSV.dial PTV @22:44:29.85 delay 5 seconds @22:44:34.87 PSV.dial PSR @22:44:35.66 delay 5 seconds @22:44:40.68 PSV.dial PTV @22:44:41.47 delay 5 seconds @22:44:46.49 PSV.dial :air @22:44:47.27 PS.seek 0ml @22:45:11.38 Additional air clear @22:45:11.39 PSR.select :air @22:45:11.40 PSV.dial PSR @22:45:12.76 Pulling 2ml of PSR.air @22:45:12.77 PSR.select :air @22:45:12.80 PS.reconfigure :default @22:45:12.84 PS.seek 2ml @22:45:34.15 delay 5 seconds @22:45:39.18 PTV.dial :puck @22:45:39.28 PSV.dial PTV @22:45:40.17 PBV.dial :waste1 @22:45:40.60 PS.seek 1.5ml @22:45:46.59 delay 3 seconds @22:45:49.61 PBV.dial between PSV and waste1 @22:45:49.98 delay 1 second @22:45:51.00 PSV.dial :waste1 @22:45:52.07 PS.seek 0ml @22:46:08.27 Processing Puck Heater at 27.9C before imaging @22:46:08.28 PH.coast @22:46:08.39 Imaging da puck in Processing Clamp while storing it to tube 3 @22:46:08.57 SC.reconfigure :tight @22:46:08.62 SC.seek 3 @22:46:12.21 SC.configure :limp @22:46:12.43 Elbow.reconfigure :tight @22:46:12.48 Elbow.seek 3 @22:46:14.76 Forearm.reconfigure :tight @22:46:14.81 Forearm.seek 3 + 450 counts @22:46:15.84 SE.reconfigure :pick @22:46:15.90 SE.seek :beamBreak @22:46:18.56 SE.configure :default @22:46:18.72 Elbow.reconfigure :limp @22:46:18.77 Forearm.reconfigure :loose @22:46:18.82 Forearm.seek 3 @22:46:19.09 Elbow.hold @22:46:19.16 Elbow.reconfigure :tight @22:46:19.27 SE.seek 7.17 Pucks below Top Plate @22:46:19.73 Forearm.force -20 @22:46:19.78 Forearm.seek 3 + 291 counts @22:46:20.07 Forearm.reconfigure :tight @22:46:20.12 Forearm.seek 3 + 450 counts @22:46:20.31 SE.seek 8.17 Pucks below Top Plate @22:46:20.85 Forearm.reconfigure :loose @22:46:20.89 Forearm.seek :retract @22:46:21.83 Elbow.seek PC @22:46:24.33 Elbow.reconfigure :loose @22:46:24.43 Forearm.seek PC @22:46:25.40 Hand.close @22:46:26.17 PC.seek :open @22:46:30.95 Forearm.seek :retract @22:46:31.95 Elbow.reconfigure :tight @22:46:32.00 Elbow.seek 3 @22:46:34.63 Forearm.reconfigure :tight @22:46:34.68 Forearm.seek 3 + 450 counts @22:46:35.63 Hand.open @22:46:36.35 SE.seek 7.82 Pucks below Top Plate @22:46:36.62 SE.reconfigure :seal @22:46:36.67 SE.seek 6.86 Pucks below Top Plate @22:46:37.75 Lights, camera, ACTION! @22:46:37.78 PuckCamera: snap -bin=2,2 -auto /var/log/esp/da23nov2722h725ml.tif @22:46:57.70 Waiting >20 seconds for da to relinquish FlushPuck @22:47:02.23 SX HX9/16 (USB:06): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 21.159 seconds /var/log/esp/da23nov2722h725ml.tif: TIFF Upload Complete @22:47:02.26 PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da23nov2722h725ml15s.tif @22:47:17.91 SX HX9/16 (USB:06): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15 seconds /var/log/esp/midres/da23nov2722h725ml15s.tif: TIFF Upload Complete @22:47:17.94 PuckCamera: snap -bin=2,2 20.0 /var/log/esp/midres/da23nov2722h725ml20s.tif @22:47:38.59 SX HX9/16 (USB:06): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 20 seconds /var/log/esp/midres/da23nov2722h725ml20s.tif: TIFF Upload Complete @22:47:38.61 Gate.power :camera,:OFF @22:47:38.63 DAfans.email "21.159 sec auto-exposure",Subject: "da23nov2722h725ml images!" @22:47:39.07 Elbow.seek 3 @22:47:39.30 Forearm.seek 3 + 450 counts @22:47:39.39 SE.reconfigure :gently @22:47:39.44 SE.seek 7.57 Pucks below Top Plate @22:47:40.09 Elbow.reconfigure :limp @22:47:40.15 Forearm.reconfigure :loose @22:47:40.22 Forearm.seek 3 @22:47:40.47 Elbow.hold @22:47:40.55 Elbow.reconfigure :tight @22:47:40.61 SE.reconfigure :default @22:47:40.68 SE.seek 7.76 Pucks below Top Plate @22:47:41.04 Forearm.force -20 @22:47:41.09 Forearm.seek 3 + 285 counts @22:47:41.38 Forearm.reconfigure :tight @22:47:41.44 Forearm.seek 3 + 450 counts @22:47:41.83 SE.seek :down @22:47:42.98 Forearm.reconfigure :loose @22:47:43.05 Forearm.seek :retract @22:47:44.05 SE.stop @22:47:44.31 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @22:47:44.88 Elbow.seek FlushPuck::Garage @22:47:47.02 Elbow.reconfigure :loose @22:47:48.22 Forearm.seek :garage @22:47:49.34 Hand.close @22:47:50.35 Forearm.seek :retract @22:47:51.46 Elbow.reconfigure :tight @22:47:51.56 Elbow.seek PC @22:47:52.93 Elbow.reconfigure :loose @22:47:53.13 Forearm.reconfigure :tight @22:47:53.23 Forearm.seek PC @22:47:55.02 PC.seek :closed @22:47:59.85 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.62 @22:47:59.99 Hand.open @22:48:00.81 Forearm.reconfigure :loose @22:48:00.90 Forearm.seek :retract @22:48:01.93 PSR.select :air @22:48:02.00 Storing wcr puck in Collection Clamp to tube 3 @22:48:02.04 PSV.dial PSR @22:48:02.76 Pulling 0.5ml of PSR.flush @22:48:02.82 PSR.select :flush @22:48:03.17 Elbow.reconfigure :tight @22:48:03.27 Elbow.seek 3 @22:48:03.39 PS.seek 0.5ml @22:48:06.06 Forearm.reconfigure :tight @22:48:06.16 Forearm.seek 3 + 450 counts @22:48:07.33 SE.reconfigure :pick @22:48:08.34 SE.seek :beamBreak @22:48:09.43 delay 5 seconds @22:48:11.11 SE.configure :default @22:48:11.44 Elbow.reconfigure :limp @22:48:11.55 Forearm.reconfigure :loose @22:48:11.64 Forearm.seek 3 @22:48:11.93 Elbow.hold @22:48:12.06 Elbow.reconfigure :tight @22:48:12.28 SE.seek 8.15 Pucks below Top Plate @22:48:12.98 Forearm.force -20 @22:48:13.07 Forearm.seek 3 + 296 counts @22:48:13.52 Forearm.reconfigure :tight @22:48:13.62 Forearm.seek 3 + 450 counts @22:48:13.86 SE.seek 9.15 Pucks below Top Plate @22:48:14.59 Forearm.reconfigure :loose @22:48:14.69 Forearm.seek :retract @22:48:14.81 PSR.select :air @22:48:15.05 PS.seek 2.5ml @22:48:15.68 Elbow.seek CC @22:48:15.97 upsync @22:48:17.32 Elbow.reconfigure :loose @22:48:17.42 Forearm.seek CC @22:48:18.40 Hand.close @22:48:19.05 CC.seek :open @22:48:23.80 Forearm.seek :retract @22:48:24.75 Elbow.reconfigure :tight @22:48:24.80 Elbow.seek 3 @22:48:27.04 Elbow.reconfigure :limp @22:48:27.10 Forearm.seek 3 @22:48:28.07 Elbow.hold @22:48:28.14 Elbow.reconfigure :tight @22:48:28.19 Hand.open @22:48:28.92 Forearm.force -20 @22:48:28.97 Forearm.seek 3 + 320 counts @22:48:29.26 Forearm.reconfigure :tight @22:48:29.31 Forearm.seek 3 + 450 counts @22:48:29.56 SE.seek :down @22:48:30.69 Forearm.reconfigure :loose @22:48:30.75 Forearm.seek :retract @22:48:31.65 SE.stop @22:48:31.71
Starting finalPurge @22:48:36.40 delay 1 second @22:48:37.42 PRV.dial :waste2,avoiding: :ab2 @22:48:39.31 PTV.dial :PRVmixing @22:48:40.64 PSV.dial :mixloop @22:48:41.68 PS.seek :empty @22:48:51.65
Waiting >20 seconds for psFlush to relinquish FlushPuck @22:49:08.21 PSR.select :air @22:49:08.23 PSV.dial PSR @22:49:09.28 Pulling 0.5ml of PSR.flush @22:49:09.29 PSR.select :flush @22:49:09.40 PS.seek 0.5ml @22:49:15.37 delay 5 seconds @22:49:20.39 PSR.select :air @22:49:20.51 PS.seek 2.5ml @22:49:41.71 delay 1 second @22:49:42.73 PBV.dial :waste1 @22:49:43.09 PTV.dial :mixing @22:49:43.84 PSV.dial :mixloop @22:49:44.90 PS.seek :empty @22:50:11.43 PSV.dial PSR @22:50:12.49 PSR.select :flush @22:50:12.62 PS.seek 1ml @22:50:23.74 PSR.select :air @22:50:23.86 PS.seek 2.5ml @22:50:40.09 PSV.dial PTV @22:50:41.41 PTV.dial :puck @22:50:42.17 PBV.dial :waste1 @22:50:42.19 PS.seek 0ml @22:51:08.55 PSV.dial PSR @22:51:09.30 PSR.select :flush @22:51:09.43 PS.seek 0.5ml @22:51:15.30 PSR.select :air @22:51:15.42 PS.seek 2.5ml @22:51:36.77 PSV.dial PTV @22:51:37.54 PBV.dial PSV @22:51:38.11 PS.seek 0ml @22:52:04.43 PSV.dial PBV @22:52:04.91 PS.seek 2.5ml @22:52:31.30 PSV.dial :waste1 @22:52:32.06 PS.seek 0ml @22:52:58.40 PSV.dial PSR @22:52:58.87 PSR.select :air @22:52:58.89 PS.seek 2.5ml @22:53:25.30 PSV.dial PTV @22:53:26.16 PBV.dial PSV @22:53:26.36 PS.seek 0ml @22:53:52.74 PSV.dial PBV @22:53:53.20 PS.seek 2.5ml @22:54:19.58 PSV.dial :waste1 @22:54:20.34 PS.seek 0ml @22:54:46.71 PSV.dial PSR @22:54:47.18 PSR.select :flush @22:54:47.30 PS.seek 1ml @22:54:58.33 PSR.select :air @22:54:58.45 PS.seek 2.5ml @22:55:14.55 PSV.dial PTV @22:55:15.41 PTV.dial :puck @22:55:15.49 PBV.dial :waste1 @22:55:16.04 PS.seek 0ml @22:55:42.39 PSV.dial PSR @22:55:43.14 PSR.select :flush @22:55:43.27 PS.seek 0.5ml @22:55:49.14 PSR.select :air @22:55:49.26 PS.seek 2.5ml @22:56:10.45 PSV.dial PTV @22:56:11.22 PBV.dial PSV @22:56:11.79 PS.seek 0ml @22:56:38.32 PSV.dial PBV @22:56:38.81 PS.seek 2.5ml @22:57:05.61 PSV.dial :waste1 @22:57:06.37 PS.seek 0ml @22:57:32.67 PSV.dial PSR @22:57:33.14 PSR.select :air @22:57:33.17 PS.seek 2.5ml @22:57:59.70 PSV.dial PTV @22:58:00.57 PBV.dial PSV @22:58:00.76 PS.seek 0ml @22:58:27.17 PSV.dial PBV @22:58:27.63 PS.seek 2.5ml @22:58:54.05 PSV.dial :waste1 @22:58:54.80 PS.seek 0ml @22:59:21.21
Loading FlushPuck into Collection Clamp from Processing Clamp @22:59:21.39 Elbow.seek PC @22:59:23.89 Elbow.reconfigure :loose @22:59:23.99 Forearm.seek PC @22:59:25.03 Hand.close @22:59:25.66 PC.seek :open @22:59:30.41 Forearm.seek :retract @22:59:31.43 Elbow.reconfigure :tight @22:59:31.48 Elbow.seek CC @22:59:35.12 Elbow.reconfigure :loose @22:59:35.22 Forearm.reconfigure :tight @22:59:35.27 Forearm.seek CC @22:59:36.57 CC.seek :closed @22:59:41.09 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.48 @22:59:41.16 Hand.open @22:59:42.29 Forearm.reconfigure :loose @22:59:42.34 Forearm.seek :retract @22:59:43.32 Flushing the Collection Valve::Manifold @22:59:43.44 CSV.dial CSR @22:59:43.88 Pulling 4.999ml of CSR.flush @22:59:43.89 CSR.select :flush @22:59:44.01 CS.seek 5ml @22:59:57.87 delay 5 seconds @23:00:02.88 CSR.select :air @23:00:03.05 CS.reconfigure :fast @23:00:03.10 CS.seek 7.5ml @23:00:09.93 CSV.dial :waste @23:00:11.23 CS.reconfigure :default @23:00:11.28 CS.seek 0ml @23:00:31.37 Flushing lower manifold's waste1 port @23:00:31.49 CSV.dial :flush @23:00:31.95 CS.reconfigure :fast @23:00:32.00 CS.seek 5ml @23:00:44.96 delay 5 seconds @23:00:49.98 CSV.dial :air @23:00:50.61 CS.seek 7ml @23:00:56.15 CSV.dial CTV @23:00:56.17
CTV.dial CSV @23:00:57.55 CBV.dial :waste1 @23:00:58.32 CS.seek 0ml @23:01:15.24 Flushing lower manifold's waste2 port @23:01:15.36 CSV.dial :flush @23:01:16.11 CS.seek 5ml @23:01:28.90 delay 5 seconds @23:01:33.92 CSV.dial :air @23:01:34.44 CS.seek 7.001ml @23:01:40.05 CSV.dial CTV @23:01:40.07
CTV.dial CSV @23:01:40.54 CBV.dial :waste2 @23:01:41.60 CS.seek 0ml @23:01:58.33 Flushing lower manifold's waste2b port @23:01:58.46 CSV.dial :flush @23:01:59.20 CS.seek 5ml @23:02:12.08 delay 5 seconds @23:02:17.10 CSV.dial :air @23:02:17.63 CS.seek 7.001ml @23:02:23.30 CSV.dial CTV @23:02:23.33
CTV.dial CSV @23:02:23.77 CBV.dial :waste2b @23:02:24.82 CS.seek 0ml @23:02:41.99 Disinfecting external Intake with kill @23:02:42.11 CSV.dial CSR @23:02:42.68 Pulling 8ml of CSR.kill @23:02:42.69 CSR.select :kill @23:02:42.81 CS.seek 8ml @23:03:02.83 delay 5 seconds @23:03:07.85 CSR.select :air @23:03:07.97 CS.seek 10ml @23:03:13.50 CSV.dial CBV @23:03:13.52
CBV.dial CSV @23:03:14.61 CTV.dial :intake @23:03:15.39 Intake.select :open @23:03:15.51 CS.seek 2ml @23:03:34.52 Intake.select :close @23:03:34.62 CTV.dial :air @23:03:35.38 CS.reconfigure :default @23:03:35.43 CS.seek 0ml @23:03:41.36 CSV.dial CSR @23:03:42.38 Pulling 5ml of CSR.flush @23:03:42.39 CSR.select :flush @23:03:42.51 CS.seek 5ml @23:03:56.33 delay 5 seconds @23:04:01.35 CSR.select :air @23:04:01.47 CS.seek 10ml @23:04:15.05 CSV.dial CBV @23:04:16.07 CS.seek 0ml @23:04:42.49 Garaging FlushPuck in Collection Clamp @23:04:42.56 Elbow.reconfigure :tight @23:04:42.61 Elbow.seek CC @23:04:42.88 Elbow.reconfigure :loose @23:04:42.98 Forearm.seek CC @23:04:44.00 Hand.close @23:04:44.66 CC.seek :open @23:04:49.29 Forearm.seek :retract @23:04:50.27 Elbow.reconfigure :tight @23:04:50.31 Elbow.seek :garage @23:04:53.21 Elbow.reconfigure :loose @23:04:53.31 Forearm.reconfigure :tight @23:04:53.36 Forearm.seek :garage @23:04:54.38 Hand.open @23:04:55.09 Forearm.reconfigure :loose @23:04:55.14 Forearm.seek :retract @23:04:56.09 Completed [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] begun 20:37:03 @23:04:56.15 Asleep after completing DA_WCR_112723b assays @23:04:56.17 GoodNews.email "{1 => 33,\n 2 => 33,\n 3 => 10,\n 4 => 23,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@22:56:27, 30% flow, 17.2C, 29% humidity, 15.2psia, 13.695V, 0.381A, 95.849Ah, 5.22W\nSampled 375.0ml",Subject: "Asleep after completing DA_WCR_112723b assays" @23:05:00.99 upsync + ESPwhich DA_WCR_112823.rb + fn=DA_WCR_112823.rb + IFS=: + set /home/esp/esp2/mission . + fullPath=/home/esp/esp2/mission/DA_WCR_112823.rb + [ -f /home/esp/esp2/mission/DA_WCR_112823.rb ] + realpath /home/esp/esp2/mission/DA_WCR_112823.rb + return 0 + scriptPath=/home/esp/esp2local/mission/DA_WCR_112823.rb + exec ruby /home/esp/esp2/lib/esp.rb /home/esp/esp2local/mission/DA_WCR_112823.rb @15:12:29.97UTC28-Nov-23
ESP2 v3.42 I2C Gateway v3.20 for core bus @15:12:32.71 Configuration for short puck jake 1/11/21 by brent@mbari.org /home/esp/esp2/type/shallow/jake/configure.rb @15:12:36.31 Polling Can every 10:00 @15:12:36.38 Cleanly exited at 15:12:09UTC28-Nov-23 @15:12:36.42 Loading /home/esp/esp2local/mission/DA_WCR_112823.rb mission @15:12:36.48 GoodNews.email "Starting DA_WCR_112823 w/pucks in tube 4\n{1 => 33,\n 2 => 33,\n 3 => 10,\n 4 => 23,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@15:12:36, 31% flow, 16.2C, 29% humidity, 15.1psia, 13.710V, 0.309A, 100.473Ah, 4.23W",Subject: "DA_WCR_112823 begun" @15:12:36.52
Preparing to sleep until + 00:00:05 @15:12:36.55 Asleep til + 00:00:05 @15:12:36.57 Continuing at 15:12 @15:12:36.85 Gate.power :core,:OFF @15:12:36.86 Gate.power :raw,:OFF @15:12:36.87 Shutdown @15:12:40.03 GoodNews.email "Then, in tube 4: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_112823.rb:14\n{1 => 33,\n 2 => 33,\n 3 => 10,\n 4 => 23,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@15:12:36, 31% flow, 16.2C, 29% humidity, 15.1psia, 13.710V, 0.315A, 100.473Ah, 4.31W",Subject: "Asleep until 15:12" @15:12:41.39 upsync @15:12:41.47
Powering core bus @15:12:44.41 upsync @15:12:47.09
Gate.power :raw,:ON @15:12:47.90 CS.configure :default @15:12:47.95 PS.configure :default @15:12:48.00 SS.configure :default @15:12:48.05 CC.configure :default @15:12:48.09 PC.configure :default @15:12:48.16 Forearm.configure :loose @15:12:48.21 Elbow.configure :loose @15:12:48.28 SC.configure :loose @15:12:48.32 SE.configure :default @15:12:48.38 CH.configure :default @15:12:48.42 PH.configure :default @15:12:48.46 All dwarf controllers running firmware version 3.85 @15:12:48.56 Valve.reset @15:12:48.89 Intake.select :close @15:12:48.90 Exhaust.select :close @15:12:48.91 CTV.dial :air @15:12:48.99 PSV.dial :air @15:12:49.04 CSV.dial :waste1 @15:12:49.17
Forearm.home.seek :retract @15:12:49.55 SSV.dial :spit,avoiding: :exhaust @15:12:49.93 Hand.close @15:12:49.97
Elbow.reconfigure :loose @15:12:50.05 Elbow.jog 1500 @15:12:50.26 PS.home.seek 0ml @15:12:50.42 CS.reconfigure :fast @15:12:50.45 SS.home.seek 0ml @15:12:50.55 CS.home.seek 0ml @15:12:50.63
Elbow.reconfigure :loose @15:12:50.68 Elbow.home.seek :clear @15:12:53.91 SE.home.seek :down @15:12:54.53 SC.reconfigure :tight @15:12:54.58 SC.home.seek :initial @15:12:58.56 PS.stop @15:13:22.90
SC.configure :limp @15:13:22.95 Forearm.seek :retract @15:13:23.02 Elbow.seek :hammer @15:13:24.09 Forearm.reconfigure :tight @15:13:24.14 Forearm.seek retracted @15:13:24.84 PC.home.seek :open @15:13:24.93
CC.home.seek :open @15:13:28.28 Phase [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] @15:13:28.30 Notice.email "At 15:13:28 began in tube 4: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_112823.rb:14\n{1 => 33,\n 2 => 33,\n 3 => 10,\n 4 => 23,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@15:13:28, 31% flow, 16.2C, 29% humidity, 15.1psia, 13.715V, 0.290A, 100.478Ah, 3.98W",Subject: "Started DA_WCR_112823 phase initialPurge; da [1000, 1000] #da and wsr are run simultanelously" @15:13:28.31
Starting initialPurge @15:13:28.34 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @15:13:28.40 Hand.open @15:13:29.18 Elbow.reconfigure :tight @15:13:29.23 Elbow.seek FlushPuck::Garage @15:13:31.16 Elbow.reconfigure :loose @15:13:31.27 Forearm.reconfigure :loose @15:13:31.33 Forearm.seek :garage @15:13:32.24 upsync @15:13:32.33
Hand.close @15:13:33.04 Forearm.seek :retract @15:13:34.01 Elbow.reconfigure :tight @15:13:34.05 Elbow.seek CC @15:13:37.24 Elbow.reconfigure :loose @15:13:37.34 Forearm.reconfigure :tight @15:13:37.39 Forearm.seek CC @15:13:38.68 CC.seek :closed @15:13:43.20 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.41 @15:13:43.27 Hand.open @15:13:43.97 Forearm.reconfigure :loose @15:13:44.02 Forearm.seek :retract @15:13:45.07 Flushing the Collection Valve::Manifold @15:13:45.19 CSV.dial CSR @15:13:46.48 Pulling 5ml of CSR.flush @15:13:46.49 CSR.select :flush @15:13:46.60 CS.reconfigure :default @15:13:46.65 CS.seek 5ml @15:14:00.95 delay 5 seconds @15:14:05.98 CSR.select :air @15:14:06.15 CS.reconfigure :fast @15:14:06.20 CS.seek 7.5ml @15:14:13.01 CSV.dial :waste @15:14:14.32 CS.reconfigure :default @15:14:14.36 CS.seek 0ml @15:14:34.36 Purging external Intake @15:14:34.53 CSV.dial :flush @15:14:34.99 CS.reconfigure :fast @15:14:35.04 CS.seek 10ml @15:14:59.58 delay 5 seconds @15:15:04.60 CSV.dial CBV @15:15:04.65
CBV.dial CSV @15:15:05.37 CTV.dial :intake @15:15:06.17 Intake.select :open @15:15:06.28 CS.seek 0ml @15:15:29.98 Intake.select :close @15:15:30.08 CTV.dial :air @15:15:30.86 Flushing upper manifold @15:15:30.98 CSV.dial :flush @15:15:31.85 CS.seek 5ml @15:15:44.58 delay 5 seconds @15:15:49.59 CSV.dial :air @15:15:50.14 CS.seek 7ml @15:15:55.64 CSV.dial CBV @15:15:55.66
CBV.dial CSV @15:15:56.71 CTV.dial :air @15:15:56.80 CS.seek 0ml @15:16:13.45 delay 7 seconds @15:16:20.48 CS.seek 3ml @15:16:28.42 CSV.dial :waste @15:16:28.88 CS.seek 0ml @15:16:36.74 CSV.dial :flush @15:16:37.19 CS.seek 3ml @15:16:45.23 delay 5 seconds @15:16:50.25 CSV.dial :air @15:16:50.76 CS.seek 5ml @15:16:56.32 CSV.dial :air @15:16:56.50 CS.seek 0ml @15:17:08.79 delay 7 seconds @15:17:15.82 CS.seek 3ml @15:17:23.92 CSV.dial :waste @15:17:24.66 CS.seek 0ml @15:17:32.45 Garaging FlushPuck in Collection Clamp @15:17:32.52 Elbow.reconfigure :tight @15:17:32.57 Elbow.seek CC @15:17:32.93 Elbow.reconfigure :loose @15:17:33.03 Forearm.seek CC @15:17:34.00 Hand.close @15:17:34.66 CC.seek :open @15:17:39.70 Forearm.seek :retract @15:17:40.66 Elbow.reconfigure :tight @15:17:40.70 Elbow.seek :garage @15:17:43.54 Elbow.reconfigure :loose @15:17:43.64 Forearm.reconfigure :tight @15:17:43.69 Forearm.seek :garage @15:17:44.68 Hand.open @15:17:45.34 Forearm.reconfigure :loose @15:17:45.39 Forearm.seek :retract @15:17:46.38 da sampling at most 1000ml, wcr at most 1000ml @15:17:46.42 Loading sh1 puck from tube 4 into Collection Clamp @15:17:46.43 DAfans.email "Started da in tube 4 at 15:17:46UTC28-Nov-23\n{1 => 33,\n 2 => 33,\n 3 => 10,\n 4 => 23,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@15:13:28, 31% flow, 16.2C, 29% humidity, 15.1psia, 13.715V, 0.290A, 100.478Ah, 3.98W",Subject: "da sampling at most 1000ml, wcr at most 1000ml" @15:17:46.97
Elbow.reconfigure :tight @15:17:47.02 Elbow.seek 4 @15:17:48.96 Forearm.reconfigure :tight @15:17:49.01 Forearm.seek 4 + 450 counts @15:17:50.02 SE.reconfigure :pick @15:17:50.09 SE.seek :beamBreak @15:17:50.57 upsync @15:17:51.70
SE.configure :default @15:17:51.87 Elbow.seek 4 @15:17:52.06 Forearm.seek 4 + 300 counts @15:17:52.28 Forearm.reconfigure :loose @15:17:52.33 Hand.close @15:17:52.92 SE.seek :down @15:17:53.12
Forearm.seek :retract @15:17:54.43 Elbow.seek CC @15:17:54.55 SE.stop @15:17:56.09
Elbow.reconfigure :loose @15:17:56.19 Forearm.reconfigure :tight @15:17:56.24 Forearm.seek CC @15:17:57.49 CC.seek :closed @15:18:02.01 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.29 @15:18:02.02 Hand.open @15:18:02.72 Forearm.reconfigure :loose @15:18:02.76 Forearm.seek :retract @15:18:03.78 collectSample 1000ml w/bubble point of 24psi @15:18:03.80 Priming 7ml @15:18:03.81 Intake.select :close @15:18:03.82 Exhaust.select :close @15:18:03.83 SS.hold @15:18:04.03 Calibrating pressure and temperature sensors @15:18:04.06 CTV.dial :air @15:18:04.08 SSV.dial :spit,avoiding: :exhaust @15:18:04.15 SS.reconfigure :fast @15:18:04.20 SS.seek 5ml @15:18:07.02 SSV.dial CBV @15:18:07.06
CBV.dial SSV @15:18:08.38 delay 3.8 seconds @15:18:12.32 Sensor limits: Intake max 103.5psia; Outlet max 96.2psia @15:18:12.33 SSV.dial :spit @15:18:13.67 SS.seek 0ml @15:18:16.46 SSV.dial CBV @15:18:17.78 CTV.dial :intake @15:18:18.56 SSV.dial CBV @15:18:18.58
CBV.dial SSV @15:18:18.67 Allowing 40s for 7.0ml of remaining 7.0ml @15:18:18.68 Intake.select :open @15:18:20.84 Intake @14.3psia, vacuum from 8.8 to 11.8psi @15:18:20.86 SS.reconfigure :inhaling 2.20ml/s @15:18:20.91 SS.seek 7ml @15:18:27.60 Stroke completed -- waiting for pressure to equalize ... @15:18:27.61 SS.reconfigure :exhale @15:18:27.66 SS.hold @15:18:37.46 Intake.select :close @15:18:37.62 SSV.dial :exhaust @15:18:38.39 Exhaust.select :open @15:18:38.50 SS.seek 0ml @15:18:42.58 Exhaust.select :close @15:18:42.69 Sampling 1000.0ml @15:18:42.70 Intake.select :close @15:18:42.71 Exhaust.select :close @15:18:42.73 SS.reconfigure :default @15:18:42.77 SS.hold @15:18:42.98 CTV.dial :intake @15:18:43.15 SSV.dial CBV @15:18:43.17
CBV.dial SSV @15:18:43.96 Allowing 130s for 25.0ml of remaining 1000.0ml @15:18:43.97 Intake.select :open @15:18:46.13 Intake @14.9psia, vacuum from 9.4 to 12.4psi @15:18:46.15 SS.reconfigure :inhaling 2.20ml/s @15:18:46.20 SS.seek 25ml @15:18:54.17 Vacuum too high -- waiting up to 40 seconds @15:18:54.18 SS.reconfigure :exhale @15:18:54.23 SS.hold @15:18:59.43 Intake @17.2psia, vacuum from 10.0 to 13.0psi @15:18:59.45 SS.reconfigure :inhaling 1.76ml/s @15:18:59.93 SS.seek 25ml @15:19:03.04 Vacuum too high -- waiting up to 40 seconds @15:19:03.06 SS.reconfigure :exhale @15:19:03.11 SS.hold @15:19:05.84 Intake @17.4psia, vacuum from 10.0 to 13.0psi @15:19:05.86 SS.reconfigure :inhaling 1.41ml/s @15:19:05.91 SS.seek 25ml @15:19:09.11 Vacuum too high -- waiting up to 40 seconds @15:19:09.12 SS.reconfigure :exhale @15:19:09.17 SS.hold @15:19:11.35 Intake @17.5psia, vacuum from 10.0 to 13.0psi @15:19:11.37 SS.reconfigure :inhaling 1.13ml/s @15:19:11.42 SS.seek 25ml @15:19:17.01 Vacuum too high -- waiting up to 40 seconds @15:19:17.03 SS.reconfigure :exhale @15:19:17.08 SS.hold @15:19:19.18 Intake @17.0psia, vacuum from 10.0 to 13.0psi @15:19:19.20 SS.reconfigure :inhaling 0.91ml/s @15:19:19.24 SS.seek 25ml @15:19:20.97 Stroke completed -- waiting for pressure to equalize ... @15:19:20.98 SS.reconfigure :exhale @15:19:21.03 SS.hold @15:19:39.75 Intake.select :close @15:19:39.90 SSV.dial :exhaust @15:19:40.68 Exhaust.select :open @15:19:40.79 SS.seek 0ml @15:19:49.49 Exhaust.select :close @15:19:49.59 SSV.dial CBV @15:19:49.61
CBV.dial SSV @15:19:50.40 Allowing 130s for 25.0ml of remaining 975.0ml @15:19:50.41 Intake.select :open @15:19:52.57 Intake @15.7psia, vacuum from 10.0 to 13.0psi @15:19:52.58 SS.reconfigure :inhaling 0.91ml/s @15:19:52.63 SS.seek 25ml @15:20:22.84 Stroke completed -- waiting for pressure to equalize ... @15:20:22.85 SS.reconfigure :exhale @15:20:22.90 SS.hold @15:20:49.85 Intake.select :close @15:20:50.00 SSV.dial :exhaust @15:20:50.82 Exhaust.select :open @15:20:50.97 SS.seek 0ml @15:20:59.77 Exhaust.select :close @15:20:59.87 SSV.dial CBV @15:20:59.89
CBV.dial SSV @15:21:00.68 Allowing 130s for 25.0ml of remaining 950.0ml @15:21:00.69 Intake.select :open @15:21:02.85 Intake @15.6psia, vacuum from 10.0 to 13.0psi @15:21:02.86 SS.reconfigure :inhaling 0.91ml/s @15:21:02.91 SS.seek 25ml @15:21:33.05 Stroke completed -- waiting for pressure to equalize ... @15:21:33.06 SS.reconfigure :exhale @15:21:33.10 SS.hold @15:21:55.88 Intake.select :close @15:21:56.03 SSV.dial :exhaust @15:21:56.79 Exhaust.select :open @15:21:56.90 SS.seek 0ml @15:22:05.91 Exhaust.select :close @15:22:06.01 SSV.dial CBV @15:22:06.03
CBV.dial SSV @15:22:06.80 Allowing 130s for 25.0ml of remaining 925.0ml @15:22:06.81 Intake.select :open @15:22:08.98 Intake @15.8psia, vacuum from 10.0 to 13.0psi @15:22:08.99 SS.reconfigure :inhaling 0.91ml/s @15:22:09.04 SS.seek 25ml @15:22:38.44 Stroke completed -- waiting for pressure to equalize ... @15:22:38.45 SS.reconfigure :exhale @15:22:38.49 SS.hold @15:23:03.39 Intake.select :close @15:23:03.54 SSV.dial :exhaust @15:23:04.33 Exhaust.select :open @15:23:04.44 SS.seek 0ml @15:23:13.20 Exhaust.select :close @15:23:13.31 SSV.dial CBV @15:23:13.33
CBV.dial SSV @15:23:14.11 Allowing 130s for 25.0ml of remaining 900.0ml @15:23:14.12 Intake.select :open @15:23:16.29 Intake @15.8psia, vacuum from 10.0 to 13.0psi @15:23:16.30 SS.reconfigure :inhaling 0.91ml/s @15:23:16.35 SS.seek 25ml @15:23:46.34 Stroke completed -- waiting for pressure to equalize ... @15:23:46.35 SS.reconfigure :exhale @15:23:46.40 SS.hold @15:24:08.78 Intake.select :close @15:24:08.93 SSV.dial :exhaust @15:24:09.70 Exhaust.select :open @15:24:09.82 SS.seek 0ml @15:24:18.50 Exhaust.select :close @15:24:18.61 SSV.dial CBV @15:24:18.63
CBV.dial SSV @15:24:19.42 Allowing 130s for 25.0ml of remaining 875.0ml @15:24:19.43 Intake.select :open @15:24:21.60 Intake @15.8psia, vacuum from 10.0 to 13.0psi @15:24:21.61 SS.reconfigure :inhaling 0.91ml/s @15:24:21.66 SS.seek 25ml @15:24:48.60 Vacuum too high -- waiting up to 40 seconds @15:24:48.61 SS.reconfigure :exhale @15:24:48.66 SS.hold @15:24:57.32 Intake @18.2psia, vacuum from 10.0 to 13.0psi @15:24:57.33 SS.reconfigure :inhaling 0.73ml/s @15:24:57.38 SS.seek 25ml @15:25:01.76 Stroke completed -- waiting for pressure to equalize ... @15:25:01.77 SS.reconfigure :exhale @15:25:01.82 SS.hold @15:25:20.35 Intake.select :close @15:25:20.50 SSV.dial :exhaust @15:25:21.26 Exhaust.select :open @15:25:21.38 SS.seek 0ml @15:25:30.09 Exhaust.select :close @15:25:30.20 SSV.dial CBV @15:25:30.22
CBV.dial SSV @15:25:31.01 Allowing 130s for 25.0ml of remaining 850.0ml @15:25:31.02 Intake.select :open @15:25:33.19 Intake @15.6psia, vacuum from 10.0 to 13.0psi @15:25:33.21 SS.reconfigure :inhaling 0.73ml/s @15:25:33.25 SS.seek 25ml @15:26:09.74 Stroke completed -- waiting for pressure to equalize ... @15:26:09.75 SS.reconfigure :exhale @15:26:09.80 SS.hold @15:26:30.28 Intake.select :close @15:26:30.44 SSV.dial :exhaust @15:26:31.21 Exhaust.select :open @15:26:31.32 SS.seek 0ml @15:26:40.28 Exhaust.select :close @15:26:40.38 SSV.dial CBV @15:26:40.40
CBV.dial SSV @15:26:41.17 Allowing 130s for 25.0ml of remaining 825.0ml @15:26:41.18 Intake.select :open @15:26:43.34 Intake @15.9psia, vacuum from 10.0 to 13.0psi @15:26:43.36 SS.reconfigure :inhaling 0.73ml/s @15:26:43.41 SS.seek 25ml @15:27:19.35 Stroke completed -- waiting for pressure to equalize ... @15:27:19.36 SS.reconfigure :exhale @15:27:19.41 SS.hold @15:27:39.71 Intake.select :close @15:27:39.86 SSV.dial :exhaust @15:27:40.64 Exhaust.select :open @15:27:40.76 SS.seek 0ml @15:27:49.68 Exhaust.select :close @15:27:49.79 SSV.dial CBV @15:27:49.81
CBV.dial SSV @15:27:50.60 Allowing 130s for 25.0ml of remaining 800.0ml @15:27:50.62 Intake.select :open @15:27:52.78 Intake @15.7psia, vacuum from 10.0 to 13.0psi @15:27:52.79 SS.reconfigure :inhaling 0.73ml/s @15:27:52.84 SS.seek 25ml @15:28:28.85 Stroke completed -- waiting for pressure to equalize ... @15:28:28.86 SS.reconfigure :exhale @15:28:28.91 SS.hold @15:28:49.22 Intake.select :close @15:28:49.37 SSV.dial :exhaust @15:28:50.13 Exhaust.select :open @15:28:50.25 SS.seek 0ml @15:28:58.84 Exhaust.select :close @15:28:58.95 SSV.dial CBV @15:28:58.98
CBV.dial SSV @15:28:59.74 Allowing 130s for 25.0ml of remaining 775.0ml @15:28:59.75 Intake.select :open @15:29:01.92 Intake @15.8psia, vacuum from 10.0 to 13.0psi @15:29:01.93 SS.reconfigure :inhaling 0.73ml/s @15:29:01.98 SS.seek 25ml @15:29:37.78 Vacuum too high -- waiting up to 40 seconds @15:29:37.79 SS.reconfigure :exhale @15:29:37.84 SS.hold @15:29:49.08 Intake @16.6psia, vacuum from 10.0 to 13.0psi @15:29:49.10 SS.reconfigure :inhaling 0.59ml/s @15:29:49.58 SS.seek 25ml @15:29:50.97 Stroke completed -- waiting for pressure to equalize ... @15:29:50.98 SS.reconfigure :exhale @15:29:51.03 SS.hold @15:30:02.11 Intake.select :close @15:30:02.26 SSV.dial :exhaust @15:30:03.02 Exhaust.select :open @15:30:03.14 SS.seek 0ml @15:30:11.90 Exhaust.select :close @15:30:12.00 SSV.dial CBV @15:30:12.02
CBV.dial SSV @15:30:12.82 Allowing 130s for 25.0ml of remaining 750.0ml @15:30:12.83 Intake.select :open @15:30:15.00 Intake @15.7psia, vacuum from 10.0 to 13.0psi @15:30:15.01 SS.reconfigure :inhaling 0.59ml/s @15:30:15.06 SS.seek 25ml @15:31:00.02 Stroke completed -- waiting for pressure to equalize ... @15:31:00.03 SS.reconfigure :exhale @15:31:00.08 SS.hold @15:31:20.99 Intake.select :close @15:31:21.15 SSV.dial :exhaust @15:31:21.91 Exhaust.select :open @15:31:22.03 SS.seek 0ml @15:31:30.75 Exhaust.select :close @15:31:30.86 SSV.dial CBV @15:31:30.89
CBV.dial SSV @15:31:31.68 Allowing 130s for 25.0ml of remaining 725.0ml @15:31:31.69 Intake.select :open @15:31:33.85 Intake @15.7psia, vacuum from 10.0 to 13.0psi @15:31:33.87 SS.reconfigure :inhaling 0.59ml/s @15:31:33.92 SS.seek 25ml @15:32:18.72 Stroke completed -- waiting for pressure to equalize ... @15:32:18.73 SS.reconfigure :exhale @15:32:18.78 SS.hold @15:32:41.54 Intake.select :close @15:32:41.69 SSV.dial :exhaust @15:32:42.46 Exhaust.select :open @15:32:42.57 SS.seek 0ml @15:32:51.35 Exhaust.select :close @15:32:51.45 SSV.dial CBV @15:32:51.48
CBV.dial SSV @15:32:52.25 Allowing 130s for 25.0ml of remaining 700.0ml @15:32:52.27 Intake.select :open @15:32:54.43 Intake @15.7psia, vacuum from 10.0 to 13.0psi @15:32:54.45 SS.reconfigure :inhaling 0.59ml/s @15:32:54.49 SS.seek 25ml @15:33:38.99 Vacuum too high -- waiting up to 40 seconds @15:33:39.01 SS.reconfigure :exhale @15:33:39.06 SS.hold @15:33:49.38 Intake @15.3psia, vacuum from 9.8 to 12.8psi @15:33:49.39 SS.reconfigure :inhaling 0.47ml/s @15:33:49.49 SS.seek 25ml @15:33:49.79 Stroke completed -- waiting for pressure to equalize ... @15:33:49.80 SS.reconfigure :exhale @15:33:49.85 SS.hold @15:34:06.15 Intake.select :close @15:34:06.30 SSV.dial :exhaust @15:34:07.06 Exhaust.select :open @15:34:07.18 SS.seek 0ml @15:34:15.88 Exhaust.select :close @15:34:15.98 SSV.dial CBV @15:34:16.02
CBV.dial SSV @15:34:16.78 Allowing 130s for 25.0ml of remaining 675.0ml @15:34:16.79 Intake.select :open @15:34:18.96 Intake @15.7psia, vacuum from 10.0 to 13.0psi @15:34:18.97 SS.reconfigure :inhaling 0.47ml/s @15:34:19.02 SS.seek 25ml @15:35:14.75 Stroke completed -- waiting for pressure to equalize ... @15:35:14.76 SS.reconfigure :exhale @15:35:14.81 SS.hold @15:35:37.43 Intake.select :close @15:35:37.58 SSV.dial :exhaust @15:35:38.34 Exhaust.select :open @15:35:38.46 SS.seek 0ml @15:35:47.25 Exhaust.select :close @15:35:47.36 SSV.dial CBV @15:35:47.38
CBV.dial SSV @15:35:48.17 Allowing 130s for 25.0ml of remaining 650.0ml @15:35:48.18 Intake.select :open @15:35:50.35 Intake @15.7psia, vacuum from 10.0 to 13.0psi @15:35:50.36 SS.reconfigure :inhaling 0.47ml/s @15:35:50.41 SS.seek 25ml @15:36:45.84 Stroke completed -- waiting for pressure to equalize ... @15:36:45.85 SS.reconfigure :exhale @15:36:45.90 SS.hold @15:37:12.46 Intake.select :close @15:37:12.62 SSV.dial :exhaust @15:37:13.37 Exhaust.select :open @15:37:13.49 SS.seek 0ml @15:37:22.29 Exhaust.select :close @15:37:22.41 SSV.dial CBV @15:37:22.43
CBV.dial SSV @15:37:23.22 Allowing 130s for 25.0ml of remaining 625.0ml @15:37:23.23 Intake.select :open @15:37:25.39 Intake @15.7psia, vacuum from 10.0 to 13.0psi @15:37:25.40 SS.reconfigure :inhaling 0.47ml/s @15:37:25.45 SS.seek 25ml @15:38:21.20 Stroke completed -- waiting for pressure to equalize ... @15:38:21.21 SS.reconfigure :exhale @15:38:21.25 SS.hold @15:38:51.96 Intake.select :close @15:38:52.11 SSV.dial :exhaust @15:38:52.87 Exhaust.select :open @15:38:52.98 SS.seek 0ml @15:39:01.79 Exhaust.select :close @15:39:01.90 SSV.dial CBV @15:39:01.92
CBV.dial SSV @15:39:02.71 Allowing 130s for 25.0ml of remaining 600.0ml @15:39:02.73 Intake.select :open @15:39:04.89 Intake @15.6psia, vacuum from 10.0 to 13.0psi @15:39:04.91 SS.reconfigure :inhaling 0.47ml/s @15:39:04.96 SS.seek 25ml @15:40:00.63 Stroke completed -- waiting for pressure to equalize ... @15:40:00.64 SS.reconfigure :exhale @15:40:00.69 SS.hold @15:40:35.54 Intake.select :close @15:40:35.70 SSV.dial :exhaust @15:40:36.46 Exhaust.select :open @15:40:36.58 SS.seek 0ml @15:40:45.66 Exhaust.select :close @15:40:45.77 SSV.dial CBV @15:40:45.80
CBV.dial SSV @15:40:46.57 Allowing 130s for 25.0ml of remaining 575.0ml @15:40:46.58 Intake.select :open @15:40:48.75 Intake @15.5psia, vacuum from 10.0 to 13.0psi @15:40:48.76 SS.reconfigure :inhaling 0.47ml/s @15:40:48.81 SS.seek 25ml @15:41:43.79 Stroke completed -- waiting for pressure to equalize ... @15:41:43.80 SS.reconfigure :exhale @15:41:43.84 SS.hold @15:42:22.97 Intake.select :close @15:42:23.12 SSV.dial :exhaust @15:42:23.90 Exhaust.select :open @15:42:24.01 SS.seek 0ml @15:42:33.26 Exhaust.select :close @15:42:33.37 SSV.dial CBV @15:42:33.41
CBV.dial SSV @15:42:34.15 Allowing 130s for 25.0ml of remaining 550.0ml @15:42:34.16 Intake.select :open @15:42:36.33 Intake @15.5psia, vacuum from 10.0 to 13.0psi @15:42:36.35 SS.reconfigure :inhaling 0.47ml/s @15:42:36.39 SS.seek 25ml @15:43:31.80 Stroke completed -- waiting for pressure to equalize ... @15:43:31.81 SS.reconfigure :exhale @15:43:31.86 SS.hold @15:44:12.03 Intake.select :close @15:44:12.19 SSV.dial :exhaust @15:44:12.94 Exhaust.select :open @15:44:13.06 SS.seek 0ml @15:44:22.11 Exhaust.select :close @15:44:22.22 SSV.dial CBV @15:44:22.25
CBV.dial SSV @15:44:23.02 Allowing 130s for 25.0ml of remaining 525.0ml @15:44:23.03 Intake.select :open @15:44:25.20 Intake @15.6psia, vacuum from 10.0 to 13.0psi @15:44:25.21 SS.reconfigure :inhaling 0.47ml/s @15:44:25.26 SS.seek 25ml @15:45:16.04 Vacuum too high -- waiting up to 40 seconds @15:45:16.05 SS.reconfigure :exhale @15:45:16.11 SS.hold @15:45:43.23 Intake @17.5psia, vacuum from 10.0 to 13.0psi @15:45:43.24 SS.reconfigure :inhaling 0.38ml/s @15:45:43.29 SS.seek 25ml @15:45:48.81 Stroke completed -- waiting for pressure to equalize ... @15:45:48.83 SS.reconfigure :exhale @15:45:48.87 SS.hold @15:46:13.37 Intake.select :close @15:46:13.53 SSV.dial :exhaust @15:46:14.73 Exhaust.select :open @15:46:14.84 SS.seek 0ml @15:46:23.58 Exhaust.select :close @15:46:23.74 SSV.dial CBV @15:46:23.81
CBV.dial SSV @15:46:24.58 Allowing 130s for 25.0ml of remaining 500.0ml @15:46:24.59 Intake.select :open @15:46:26.76 Intake @15.6psia, vacuum from 10.0 to 13.0psi @15:46:26.78 SS.reconfigure :inhaling 0.38ml/s @15:46:26.83 SS.seek 25ml @15:47:35.65 Stroke completed -- waiting for pressure to equalize ... @15:47:35.66 SS.reconfigure :exhale @15:47:35.71 SS.hold @15:48:15.89 Intake.select :close @15:48:16.05 SSV.dial :exhaust @15:48:16.81 Exhaust.select :open @15:48:16.93 SS.seek 0ml @15:48:25.79 Exhaust.select :close @15:48:25.90 SSV.dial CBV @15:48:25.93
CBV.dial SSV @15:48:26.74 Allowing 130s for 25.0ml of remaining 475.0ml @15:48:26.75 Intake.select :open @15:48:28.92 Intake @15.7psia, vacuum from 10.0 to 13.0psi @15:48:28.93 SS.reconfigure :inhaling 0.38ml/s @15:48:28.98 SS.seek 25ml @15:49:36.82 Vacuum too high -- waiting up to 40 seconds @15:49:36.83 SS.reconfigure :exhale @15:49:36.88 SS.hold @15:50:03.66 Intake @17.3psia, vacuum from 10.0 to 13.0psi @15:50:03.67 SS.reconfigure :inhaling 0.31ml/s @15:50:03.72 SS.seek 25ml @15:50:05.79 Stroke completed -- waiting for pressure to equalize ... @15:50:05.80 SS.reconfigure :exhale @15:50:05.85 SS.hold @15:50:30.35 Intake.select :close @15:50:30.94 SSV.dial :exhaust @15:50:31.70 Exhaust.select :open @15:50:31.86 SS.seek 0ml @15:50:40.75 Exhaust.select :close @15:50:40.85 SSV.dial CBV @15:50:40.88
CBV.dial SSV @15:50:41.67 Allowing 130s for 25.0ml of remaining 450.0ml @15:50:41.68 Intake.select :open @15:50:43.85 Intake @15.5psia, vacuum from 10.0 to 13.0psi @15:50:43.86 SS.reconfigure :inhaling 0.31ml/s @15:50:43.91 SS.seek 25ml @15:52:14.04 Stroke completed -- waiting for pressure to equalize ... @15:52:14.05 SS.reconfigure :exhale @15:52:14.10 SS.hold @15:52:51.69 SS.reconfigure :default @15:52:51.74 SS.hold @15:52:51.85 Intake.select :close @15:52:52.06 ShallowSampler::Clogged in MAIN -- Filtering too Slowly after 575.0ml @15:52:52.07 SSV.dial :exhaust @15:52:52.84 Exhaust.select :open @15:52:52.94 SS.reconfigure :exhale @15:52:52.99 SS.seek 0ml @15:53:01.85 Exhaust.select :close @15:53:01.96 Sampled 575.0ml @15:53:01.98 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) Intake.select :close @15:53:02.05 CSV.dial :waste @15:53:02.22 SSV.dial CBV,avoiding: :exhaust @15:53:02.24 CS.seek 0ml @15:53:02.26
CBV.dial SSV @15:53:02.38 CSV.dial :air @15:53:03.14 CS.seek 10ml @15:53:04.20
CTV.dial :air @15:53:04.96 delay 15 seconds @15:53:19.98 SSV.dial :spit,avoiding: :exhaust @15:53:21.33 CTV.dial CSV,avoiding: :intake @15:53:21.36
SS.seek 0ml @15:53:21.48 SSV.dial CBV,avoiding: :exhaust @15:53:27.94 SSV.dial between CBV and plug @15:53:28.43 CBV.dial between SSV and waste2 @15:53:28.81 CSV.dial between air and CTV @15:53:29.12 CS.seek 8.461ml @15:53:33.49 CTV.dial CSV,avoiding: :air @15:53:33.51
CSV.dial CTV @15:53:33.87 CS.reconfigure :pressurize @15:53:33.92 CS.seek 0ml @15:53:49.42 BubblePoint: Intake @24.1psia, Outlet @0.9psia @15:53:49.43 CBV.dial SSV @15:53:49.77 SS.reconfigure :default @15:53:49.82 SS.seek 10ml @15:53:55.46 delay 15 seconds @15:54:10.59 Evacuated: Intake @23.7psia, Outlet @23.6psia @15:54:10.60 SSV.dial :spit,avoiding: :exhaust @15:54:11.70 SS.reconfigure :exhale @15:54:11.75 SS.seek 0ml @15:54:16.72 SS.reconfigure :fast @15:54:16.77 SS.seek 5ml @15:54:19.69 SSV.dial CBV,avoiding: :exhaust @15:54:19.70
CBV.dial SSV @15:54:21.09 CTV.dial :air,avoiding: :intake @15:54:22.44 CSV.dial CTV @15:54:22.48
SS.seek 10ml @15:54:23.70 CSV.dial :air @15:54:24.13 CS.reconfigure :fast @15:54:24.18 CS.seek 0ml @15:54:25.13
SSV.dial :spit,avoiding: :exhaust @15:54:26.48 SS.reconfigure :exhale @15:54:26.53 SS.seek 0ml @15:54:31.06 CS.stop @15:54:31.72
SS.reconfigure :fast @15:54:31.77 SS.seek 5ml @15:54:34.53 SSV.dial CBV,avoiding: :exhaust @15:54:34.55
CBV.dial SSV @15:54:36.92 SSV.dial :spit,avoiding: :exhaust @15:54:38.25 SS.reconfigure :default @15:54:38.29 SS.seek 0ml @15:54:41.57 SS.stop @15:54:41.61 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @15:54:41.62 Intake.select :close @15:54:41.68 CSV.dial :waste @15:54:41.82 SSV.dial CBV,avoiding: :exhaust @15:54:41.84
CBV.dial SSV @15:54:42.44 CS.seek 0ml @15:54:42.51 CSV.dial :air @15:54:43.20
CTV.dial :air @15:54:43.38 delay 15 seconds @15:54:43.41 CS.seek 10ml @15:54:58.41
SSV.dial :spit,avoiding: :exhaust @15:54:59.76 CTV.dial CSV,avoiding: :intake @15:54:59.79
SS.reconfigure :exhale @15:54:59.89 SS.seek 0ml @15:55:00.32 SSV.dial CBV,avoiding: :exhaust @15:55:07.97 SSV.dial between CBV and plug @15:55:08.46 CBV.dial between SSV and waste2 @15:55:08.86 CSV.dial between air and CTV @15:55:09.31 CS.seek 7.449ml @15:55:15.95 CTV.dial CSV,avoiding: :air @15:55:15.99
CSV.dial CTV @15:55:16.71 CS.reconfigure :pressurize @15:55:16.82 CS.seek 0ml @15:55:32.28 BubblePoint: Intake @24.1psia, Outlet @3.5psia @15:55:32.29 CBV.dial SSV @15:55:32.62 SS.reconfigure :default @15:55:32.66 SS.seek 10ml @15:55:38.32 delay 15 seconds @15:55:53.45 Evacuated: Intake @23.9psia, Outlet @23.8psia @15:55:53.46 SSV.dial :spit,avoiding: :exhaust @15:55:54.51 SS.reconfigure :exhale @15:55:54.56 SS.seek 0ml @15:55:59.53 SS.reconfigure :fast @15:55:59.58 SS.seek 5ml @15:56:02.53 SSV.dial CBV,avoiding: :exhaust @15:56:02.55
CBV.dial SSV @15:56:03.90 CTV.dial :air,avoiding: :intake @15:56:05.24 CSV.dial CTV @15:56:05.27
SS.seek 10ml @15:56:06.49 CSV.dial :air @15:56:06.93 CS.reconfigure :fast @15:56:06.98 CS.seek 0ml @15:56:07.97
SSV.dial :spit,avoiding: :exhaust @15:56:09.31 SS.reconfigure :exhale @15:56:09.36 SS.seek 0ml @15:56:11.75 CS.stop @15:56:14.53
SS.reconfigure :fast @15:56:14.58 SS.seek 5ml @15:56:17.51 SSV.dial CBV,avoiding: :exhaust @15:56:17.53
CBV.dial SSV @15:56:19.87 SSV.dial :spit,avoiding: :exhaust @15:56:21.21 SS.reconfigure :default @15:56:21.26 SS.seek 0ml @15:56:24.36 SS.stop @15:56:24.40 Lysing: Adding 2.0ml AqMeOH @15:56:24.42 CBV.dial between CSV and waste1,avoiding: :DBV @15:56:25.94 CTV.dial CSV @15:56:27.27 CSV.dial CSR @15:56:28.06 Pulling 1ml of CSR.AqMeOH CSR.select :AqMeOH @15:56:28.17 CS.reconfigure :default @15:56:28.22 CS.seek 1ml @15:56:31.84 delay 5 seconds @15:56:36.86 CSR.select :air @15:56:37.02 CS.seek 6.001ml @15:56:50.62 delay 2 seconds @15:56:52.64 CSV.dial :waste2 @15:56:53.21 CS.reconfigure :fast @15:56:53.26 CS.seek 0ml @15:57:07.62 CSV.dial CSR @15:57:08.48 Pulling 2ml of CSR.AqMeOH @15:57:08.49 CSR.select :AqMeOH @15:57:08.61 CS.reconfigure :default @15:57:08.66 CS.seek 2ml @15:57:14.81 delay 5 seconds @15:57:19.83 CSR.select :air @15:57:20.00 CS.seek 5ml @15:57:28.50 delay 2 seconds @15:57:30.52 CSV.dial CTV @15:57:30.97 CS.seek 1ml @15:57:42.36 Before lysing Collection Puck Heater at 13.7C/31.1psia @15:57:42.38 Heating Puck.from 4, :sh1 in Collection Clamp to 70C for 10:00 @15:57:42.40 CH.seek 70C,8:00 @15:59:53.99 CH.hold 10:00,70C @15:59:54.19 Begun lysing Collection Puck Heater at 68C/38.0psia @16:09:54.19 Ended lysing Collection Puck Heater at 70C/37.2psia @16:09:54.21 CS.seek 0ml @16:09:57.87 CTV.dial between spare and air,avoiding: :intake @16:09:58.88 CSV.dial :air @16:09:59.33 CS.seek 5ml @16:10:12.92 CSV.dial CTV @16:10:13.38 delay 2 seconds @16:10:15.40 CSV.dial :air @16:10:15.85 delay 2 seconds @16:10:17.86 CSV.dial CTV @16:10:18.32 delay 2 seconds @16:10:20.34 CSV.dial :air @16:10:20.78 delay 2 seconds @16:10:22.81 CS.seek 0ml @16:10:36.51 Puck @84.0psia after final lysis pressurization @16:10:36.52 Recovering Lysate into Collection syringe @16:10:36.53 CSV.dial CBV @16:10:37.55 CBV.dial CSV @16:10:37.92 CS.seek 5ml @16:10:51.61 delay 5 seconds @16:10:56.63 CTV.dial :air @16:10:57.15 CS.seek 7ml @16:11:03.08 delay 5 seconds @16:11:08.10 CSV.dial :air2 @16:11:08.55 CS.seek 10ml @16:11:17.08 CSV.dial between air and CTV,avoiding: CBV @16:11:18.24 Lysing Complete @16:11:18.77 Storing sh1 puck in Collection Clamp to tube 3 @16:11:18.95 SC.reconfigure :tight @16:11:19.00 SC.seek 3 @16:11:22.58 SC.configure :limp @16:11:22.79 Elbow.reconfigure :tight @16:11:22.84 Elbow.seek 3 @16:11:24.62 Forearm.reconfigure :tight @16:11:24.67 Forearm.seek 3 + 450 counts @16:11:25.73 SE.reconfigure :pick @16:11:25.79 SE.seek :beamBreak @16:11:28.29 SE.configure :default @16:11:28.45 Elbow.reconfigure :limp @16:11:28.50 Forearm.reconfigure :loose @16:11:28.55 Forearm.seek 3 @16:11:28.79 Elbow.hold @16:11:28.86 Elbow.reconfigure :tight @16:11:28.96 SE.seek 9.15 Pucks below Top Plate @16:11:29.35 Forearm.force -20 @16:11:29.40 Forearm.seek 3 + 279 counts @16:11:29.68 Forearm.reconfigure :tight @16:11:29.73 Forearm.seek 3 + 450 counts @16:11:30.00 SE.seek 10.15 Pucks below Top Plate @16:11:30.59 Forearm.reconfigure :loose @16:11:30.64 Forearm.seek :retract @16:11:31.53 Elbow.seek CC @16:11:33.21 Elbow.reconfigure :loose @16:11:33.31 Forearm.seek CC @16:11:34.34 Hand.close @16:11:34.99 CC.seek :open @16:11:40.19 Forearm.seek :retract @16:11:41.17 Elbow.reconfigure :tight @16:11:41.22 Elbow.seek 3 @16:11:42.99 Elbow.reconfigure :limp @16:11:43.04 Forearm.seek 3 @16:11:44.09 Elbow.hold @16:11:44.16 Elbow.reconfigure :tight @16:11:44.20 Hand.open @16:11:44.98 Forearm.force -20 @16:11:45.03 Forearm.seek 3 + 309 counts @16:11:45.32 Forearm.reconfigure :tight @16:11:45.37 Forearm.seek 3 + 450 counts @16:11:45.61 SE.seek :down @16:11:46.75
Forearm.reconfigure :loose @16:11:46.80 Forearm.seek :retract @16:11:47.63 SE.stop @16:11:47.73
Tranferring DA @16:11:47.75 Beginning samplePassC @16:11:47.76 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @16:11:47.95 Elbow.seek FlushPuck::Garage @16:11:49.84 Elbow.reconfigure :loose @16:11:49.95 Forearm.seek :garage @16:11:50.90 Hand.close @16:11:51.60 Forearm.seek :retract @16:11:52.55 Elbow.reconfigure :tight @16:11:52.59 Elbow.seek CC @16:11:55.58 Elbow.reconfigure :loose @16:11:55.68 Forearm.reconfigure :tight @16:11:55.73 Forearm.seek CC @16:11:57.41 CC.seek :closed @16:12:01.93 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.39 @16:12:02.00 Hand.open @16:12:02.67 Forearm.reconfigure :loose @16:12:02.72 Forearm.seek :retract @16:12:03.68 PSV.dial PSR @16:12:05.10 Pulling 1.5ml of PSR.flush PSR.select :flush @16:12:05.22 PS.seek 1.5ml @16:12:21.43 delay 5 seconds @16:12:26.45 PSV.dial :waste1 @16:12:26.92 PS.seek 0ml @16:12:43.11 PSV.dial PSR @16:12:43.58 PSR.select :air @16:12:43.70 PS.seek 2.5ml @16:13:10.06 PRV.dial CBV,avoiding: :ab2 @16:13:10.08
CBV.dial PRV @16:13:11.85 PSV.dial :mixloop @16:13:12.90 CTV.dial :air,avoiding: :intake @16:13:13.12 PTV.dial :PRVmixing @16:13:14.47 PS.seek 0ml @16:13:40.93 PSV.dial :air @16:13:41.40 PS.seek 2.5ml @16:14:07.74 PSV.dial :mixloop @16:14:08.23 PS.seek 0ml @16:14:34.61 PSV.dial :air @16:14:35.09 PS.seek 2.5ml @16:15:01.52 PSV.dial :mixloop @16:15:01.99 PS.seek 0ml @16:15:28.36 Garaging FlushPuck in Collection Clamp @16:15:28.82 Elbow.reconfigure :tight @16:15:28.93 Elbow.seek CC @16:15:29.26 Elbow.reconfigure :loose @16:15:29.36 Forearm.seek CC @16:15:30.40 Hand.close @16:15:30.98 CC.seek :open @16:15:35.63 Forearm.seek :retract @16:15:36.68 Elbow.reconfigure :tight @16:15:36.73 Elbow.seek :garage @16:15:39.60 Elbow.reconfigure :loose @16:15:39.70 Forearm.reconfigure :tight @16:15:39.75 Forearm.seek :garage @16:15:40.82 Hand.open @16:15:41.54 Forearm.reconfigure :loose @16:15:41.59 Forearm.seek :retract @16:15:42.56 PSV.dial :mixloop @16:15:42.63 PTV.dial :PRVmixing @16:15:42.82 PRV.dial CBV @16:15:42.84
CBV.dial PRV @16:15:43.06 CTV.dial :air,avoiding: :intake @16:15:43.08 Loading lyfil puck from tube 4 into Collection Clamp @16:15:43.32 SC.reconfigure :tight @16:15:43.36 SC.seek 4 @16:15:46.96 SC.configure :limp @16:15:47.17 Elbow.reconfigure :tight @16:15:47.22 Elbow.seek 4 @16:15:49.19 Forearm.reconfigure :tight @16:15:49.24 Forearm.seek 4 + 450 counts @16:15:50.26 SE.reconfigure :pick @16:15:50.32 SE.seek :beamBreak @16:15:52.03 SE.configure :default @16:15:52.68 Elbow.seek 4 @16:15:52.92 Forearm.seek 4 + 300 counts @16:15:53.12 Forearm.reconfigure :loose @16:15:53.17 Hand.close @16:15:53.74 SE.seek :down @16:15:53.95
Forearm.seek :retract @16:15:54.90 Elbow.seek CC @16:15:55.13 SE.stop @16:15:56.52
Elbow.reconfigure :loose @16:15:56.62 Forearm.reconfigure :tight @16:15:56.67 Forearm.seek CC @16:15:57.94 CC.seek :closed @16:16:02.46 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.13 @16:16:02.47 Hand.open @16:16:03.17 Forearm.reconfigure :loose @16:16:03.21 Forearm.seek :retract @16:16:04.21 CTV.dial CSV,avoiding: :intake @16:16:05.54 CSV.dial CTV @16:16:05.88 PS.seek 2.5ml @16:16:32.26 CS.seek 4.5ml @16:16:47.10 delay 8 seconds @16:16:55.13 PSV.dial :air @16:16:55.60 delay 2 seconds @16:16:57.61 PSV.dial :waste2 @16:16:58.37 PS.seek 0ml @16:17:24.96 CBV.dial between PRV and waste1 @16:17:25.29 CSV.dial :air @16:17:25.77 CS.seek 10ml @16:17:40.63 CSV.dial CTV @16:17:41.09 CS.seek 0ml @16:18:07.73 CBV.dial :waste1 @16:18:08.04 delay 5 seconds @16:18:13.06 CTV.dial :air,avoiding: :intake @16:18:14.46 Storing lyfil puck in Collection Clamp to tube 3 @16:18:14.64 SC.reconfigure :tight @16:18:14.69 SC.seek 3 @16:18:18.31 SC.configure :limp @16:18:18.52 Elbow.reconfigure :tight @16:18:18.57 Elbow.seek 3 @16:18:20.36 Forearm.reconfigure :tight @16:18:20.41 Forearm.seek 3 + 450 counts @16:18:21.40 SE.reconfigure :pick @16:18:21.45 SE.seek :beamBreak @16:18:23.96 SE.configure :default @16:18:24.12 Elbow.reconfigure :limp @16:18:24.17 Forearm.reconfigure :loose @16:18:24.22 Forearm.seek 3 @16:18:24.49 Elbow.hold @16:18:24.56 Elbow.reconfigure :tight @16:18:24.66 SE.seek 10.13 Pucks below Top Plate @16:18:25.11 Forearm.force -20 @16:18:25.15 Forearm.seek 3 + 295 counts @16:18:25.44 Forearm.reconfigure :tight @16:18:25.49 Forearm.seek 3 + 450 counts @16:18:25.67 SE.seek 11.13 Pucks below Top Plate @16:18:26.64 Forearm.reconfigure :loose @16:18:26.69 Forearm.seek :retract @16:18:27.64 Elbow.seek CC @16:18:29.33 Elbow.reconfigure :loose @16:18:29.43 Forearm.seek CC @16:18:30.40 Hand.close @16:18:30.98 CC.seek :open @16:18:35.71 Forearm.seek :retract @16:18:36.72 Elbow.reconfigure :tight @16:18:36.76 Elbow.seek 3 @16:18:38.55 Elbow.reconfigure :limp @16:18:38.60 Forearm.seek 3 @16:18:39.57 Elbow.hold @16:18:39.64 Elbow.reconfigure :tight @16:18:39.69 Hand.open @16:18:40.47 Forearm.force -20 @16:18:40.52 Forearm.seek 3 + 297 counts @16:18:40.82 Forearm.reconfigure :tight @16:18:40.87 Forearm.seek 3 + 450 counts @16:18:41.11 SE.seek :down @16:18:42.24
Forearm.reconfigure :loose @16:18:42.28 Forearm.seek :retract @16:18:43.03 SE.stop @16:18:43.27
PSV.dial :air @16:18:44.02 PS.seek 2.5ml @16:19:10.39 delay 1 second @16:19:11.41 PSV.dial :mixloop @16:19:11.86 delay 1 second @16:19:12.87 PSV.dial :air @16:19:13.41 delay 1 second @16:19:14.42 PSV.dial :mixloop @16:19:14.89 delay 1 second @16:19:15.91 PSV.dial :air @16:19:16.38 delay 1 second @16:19:17.40 PS.seek 0ml @16:19:43.77 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @16:19:43.96 Elbow.seek FlushPuck::Garage @16:19:45.88 Elbow.reconfigure :loose @16:19:45.98 Forearm.seek :garage @16:19:46.93 Hand.close @16:19:47.72 Forearm.seek :retract @16:19:48.73 Elbow.reconfigure :tight @16:19:48.79 Elbow.seek PC @16:19:50.06 Elbow.reconfigure :loose @16:19:50.16 Forearm.reconfigure :tight @16:19:50.21 Forearm.seek PC @16:19:51.44 PC.seek :closed @16:19:56.09 Processing Clamp onSpring/offSpring for 16/26 samples, on/off torque 1.64 @16:19:56.16 Hand.open @16:19:56.79 Forearm.reconfigure :loose @16:19:56.83 Forearm.seek :retract @16:19:57.77 PRV.dial :air @16:19:58.23 PSV.dial :mixloop @16:19:58.76 PS.seek 0.3ml @16:20:02.69 PSV.dial PSR @16:20:03.75 Pulling 0.4ml of PSR.air @16:20:03.76 PSR.select :air @16:20:03.78 PS.seek 0.7ml @16:20:08.73 delay 3 seconds @16:20:11.75 PSR.select :air @16:20:11.77 PS.seek 2.5ml @16:20:31.01 delay 3 seconds @16:20:34.03 PSV.dial :mixloop @16:20:35.09 PTV.dial :mixing @16:20:35.86 PBV.dial :waste2 @16:20:36.83 PS.seek 0ml @16:21:03.11 Garaging FlushPuck in Processing Clamp @16:21:03.18 Elbow.reconfigure :tight @16:21:03.23 Elbow.seek PC @16:21:03.35 Elbow.reconfigure :loose @16:21:03.45 Forearm.seek PC @16:21:04.46 Hand.close @16:21:05.10 PC.seek :open @16:21:09.82 Forearm.seek :retract @16:21:10.76 Elbow.reconfigure :tight @16:21:10.80 Elbow.seek :garage @16:21:12.17 Elbow.reconfigure :loose @16:21:12.27 Forearm.reconfigure :tight @16:21:12.32 Forearm.seek :garage @16:21:13.33 Hand.open @16:21:14.03 Forearm.reconfigure :loose @16:21:14.08 Forearm.seek :retract @16:21:15.07 Loading da puck from tube 4 into Processing Clamp @16:21:15.30 SC.reconfigure :tight @16:21:15.35 SC.seek 4 @16:21:18.94 SC.configure :limp @16:21:19.15 Elbow.reconfigure :tight @16:21:19.20 Elbow.seek 4 @16:21:21.08 Forearm.reconfigure :tight @16:21:21.13 Forearm.seek 4 + 450 counts @16:21:22.60 SE.reconfigure :pick @16:21:22.66 SE.seek :beamBreak @16:21:24.52 SE.configure :default @16:21:24.68 Elbow.seek 4 @16:21:24.87 Forearm.seek 4 + 300 counts @16:21:25.01 Forearm.reconfigure :loose @16:21:25.05 Hand.close @16:21:25.74 SE.seek :down @16:21:25.94
Forearm.seek :retract @16:21:26.91 Elbow.seek PC @16:21:27.15 SE.stop @16:21:29.50
Elbow.reconfigure :loose @16:21:29.60 Forearm.reconfigure :tight @16:21:29.65 Forearm.seek PC @16:21:30.84 PC.seek :closed @16:21:35.51 Processing Clamp onSpring/offSpring for 16/26 samples, on/off torque 1.64 @16:21:35.52 Hand.open @16:21:36.17 Forearm.reconfigure :loose @16:21:36.21 Forearm.seek :retract @16:21:37.17 Heating Puck.from 4, :da in Processing Clamp to 28C for 2:00:00 @16:21:37.19 PH.seek 28C,5:00 @16:22:21.98 PH.hold 2:00:00,28C @16:22:22.04 delay 1:30 @16:23:52.08 PSR.select :air @16:23:52.09 PSV.dial PSR @16:23:53.15 Pulling 0.5ml of PSR.wash @16:23:53.16 PSR.select :wash @16:23:53.28 PS.seek 0.5ml @16:23:59.29 delay 5 seconds @16:24:04.31 PSR.select :air @16:24:04.43 PS.seek 2ml @16:24:20.66 PTV.dial :puck @16:24:21.41 PSV.dial PTV @16:24:22.18 PBV.dial :waste1 @16:24:23.59 PS.seek 1.5ml @16:24:29.57 delay 3 seconds @16:24:32.59 PBV.dial between PSV and waste1 @16:24:32.96 PS.seek 0ml @16:24:49.04 delay 5:00 @16:29:49.06 recoverPSRreagent08 @16:29:49.07 PTV.dial :puck @16:29:49.14 PBV.dial :waste1 @16:29:49.52 delay 30 seconds @16:30:19.99 PBV.dial between PSV and waste1 @16:30:20.36 PSR.select :air @16:30:20.38 PSV.dial PSR @16:30:21.13 PS.reconfigure :fast @16:30:21.18 PS.seek 2.5ml @16:30:45.00 PSV.dial PTV @16:30:45.77 delay 5 seconds @16:30:50.79 PSV.dial PSR @16:30:51.57 delay 5 seconds @16:30:56.59 PSV.dial PTV @16:30:57.35 delay 5 seconds @16:31:02.37 PSV.dial :air @16:31:03.14 PS.seek 0ml @16:31:26.28 PTV.dial :PRVmixing,via: PRV @16:31:27.63 PSV.dial :mixloop @16:31:28.09 PRV.dial :ab1 @16:31:28.85 PS.reconfigure :slow @16:31:28.90 PS.seek 0.15ml @16:31:32.44 delay 2 seconds @16:31:34.46 CBV.dial PRV @16:31:34.48
PRV.dial CBV @16:31:35.05 PS.seek 0.35ml @16:31:39.66 delay 2 seconds @16:31:41.67 CBV.dial between PRV and PSV @16:31:42.00 PRV.dial :air @16:31:42.46 PS.seek 0.9ml @16:31:54.25 PRV.dial :waste2 @16:31:55.01 PS.seek 0.5ml @16:32:03.72 addPMLReagent08 @16:32:03.73 PSV.dial :air @16:32:04.19 PS.reconfigure :default @16:32:04.24 PS.seek 2ml @16:32:20.47 PSV.dial :PML @16:32:20.94 PTV.dial :mixing @16:32:21.69 PBV.dial :waste1 @16:32:22.12 PS.seek 1.85ml @16:32:24.47 PBV.dial between PSV and waste1 @16:32:24.83 PS.seek 0ml @16:32:44.72 delay 2 seconds @16:32:46.74 Beginning samplePassC @16:32:46.76 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @16:32:46.94 Elbow.reconfigure :tight @16:32:46.98 Elbow.seek FlushPuck::Garage @16:32:48.43 Elbow.reconfigure :loose @16:32:48.58 Forearm.seek :garage @16:32:49.97 Hand.close @16:32:50.72 Forearm.seek :retract @16:32:51.66 Elbow.reconfigure :tight @16:32:51.70 Elbow.seek CC @16:32:54.60 Elbow.reconfigure :loose @16:32:54.70 Forearm.reconfigure :tight @16:32:54.75 Forearm.seek CC @16:32:56.05 CC.seek :closed @16:33:00.59 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.40 @16:33:00.66 Hand.open @16:33:01.28 Forearm.reconfigure :loose @16:33:01.33 Forearm.seek :retract @16:33:02.26 PSV.dial PSR @16:33:03.33 Pulling 1.5ml of PSR.flush @16:33:03.34 PSR.select :flush @16:33:03.46 PS.seek 1.5ml @16:33:19.69 delay 5 seconds @16:33:24.71 PSV.dial :waste1 @16:33:25.16 PS.seek 0ml @16:33:41.28 PSV.dial PSR @16:33:41.75 PSR.select :air @16:33:41.87 PS.seek 2.5ml @16:34:08.44 PRV.dial CBV,avoiding: :ab2 @16:34:08.46
CBV.dial PRV @16:34:10.09 PSV.dial :mixloop @16:34:11.14 CTV.dial :air,avoiding: :intake @16:34:11.32 PTV.dial :PRVmixing @16:34:12.11 PS.seek 0ml @16:34:39.00 PSV.dial :air @16:34:39.47 PS.seek 2.5ml @16:35:05.84 PSV.dial :mixloop @16:35:06.33 PS.seek 0ml @16:35:32.87 PSV.dial :air @16:35:33.35 PS.seek 2.5ml @16:35:59.87 PSV.dial :mixloop @16:36:00.35 PS.seek 0ml @16:36:26.84 Garaging FlushPuck in Collection Clamp @16:36:26.91 Elbow.reconfigure :tight @16:36:26.96 Elbow.seek CC @16:36:27.29 Elbow.reconfigure :loose @16:36:27.39 Forearm.seek CC @16:36:28.43 Hand.close @16:36:29.09 CC.seek :open @16:36:33.70 Forearm.seek :retract @16:36:34.70 Elbow.reconfigure :tight @16:36:34.75 Elbow.seek :garage @16:36:37.71 Elbow.reconfigure :loose @16:36:37.81 Forearm.reconfigure :tight @16:36:37.86 Forearm.seek :garage @16:36:38.80 Hand.open @16:36:39.52 Forearm.reconfigure :loose @16:36:39.57 Forearm.seek :retract @16:36:40.54 delayUntil "16:40:46.73" @16:36:40.57 Whole Cell Archive sampling 1000ml @16:36:40.59 Loading wcr puck from tube 4 into Collection Clamp @16:36:40.83 SC.reconfigure :tight @16:36:40.88 SC.seek 4 @16:36:40.97 SC.configure :limp @16:36:41.19 Elbow.reconfigure :tight @16:36:41.24 Elbow.seek 4 @16:36:43.65 Forearm.reconfigure :tight @16:36:43.70 Forearm.seek 4 + 450 counts @16:36:44.85 SE.reconfigure :pick @16:36:44.91 SE.seek :beamBreak @16:36:46.76 SE.configure :default @16:36:46.93 Elbow.seek 4 @16:36:47.12 Forearm.seek 4 + 300 counts @16:36:47.27 Forearm.reconfigure :loose @16:36:47.32 Hand.close @16:36:47.98 SE.seek :down @16:36:48.18 Forearm.seek :retract @16:36:49.14 Elbow.seek CC @16:36:49.46 SE.stop @16:36:50.77 Elbow.reconfigure :loose @16:36:50.87 Forearm.reconfigure :tight @16:36:50.92 Forearm.seek CC @16:36:52.14 CC.seek :closed @16:36:56.69 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.34 @16:36:56.70 Hand.open @16:36:57.40 Forearm.reconfigure :loose @16:36:57.45 Forearm.seek :retract @16:36:58.92 collectSample 1000ml w/bubble point of 35psi @16:36:58.93 Priming 7ml @16:36:58.94 Intake.select :close @16:36:58.95 Exhaust.select :close @16:36:58.97 SS.hold @16:36:59.18 Calibrating pressure sensors @16:36:59.19 CTV.dial :air @16:36:59.21 SSV.dial :spit,avoiding: :exhaust @16:36:59.29 SS.reconfigure :fast @16:36:59.34 SS.seek 5ml @16:37:01.99 SSV.dial CBV @16:37:02.01 CBV.dial SSV @16:37:03.39 delay 3.8 seconds @16:37:07.33 Sensor limits: Intake max 104.7psia; Outlet max 96.2psia @16:37:07.34 SSV.dial :spit @16:37:08.68 SS.seek 0ml @16:37:11.55 SSV.dial CBV @16:37:12.88 CTV.dial :intake @16:37:13.65 SSV.dial CBV @16:37:13.67 CBV.dial SSV @16:37:13.82 Allowing 40s for 7.0ml of remaining 7.0ml @16:37:13.84 Intake.select :open @16:37:16.00 Intake @14.1psia, vacuum from 8.6 to 11.6psi @16:37:16.02 SS.reconfigure :inhaling 2.20ml/s @16:37:16.07 SS.seek 7ml @16:37:23.00 Stroke completed -- waiting for pressure to equalize ... @16:37:23.01 SS.reconfigure :exhale @16:37:23.06 SS.hold @16:37:37.46 Intake.select :close @16:37:37.62 SSV.dial :exhaust @16:37:38.38 Exhaust.select :open @16:37:38.49 SS.seek 0ml @16:37:42.61 Exhaust.select :close @16:37:42.72 Sampling 1000.0ml @16:37:42.74 Intake.select :close @16:37:42.75 Exhaust.select :close @16:37:42.76 SS.reconfigure :default @16:37:42.81 SS.hold @16:37:43.03 CTV.dial :intake @16:37:43.10 SSV.dial CBV @16:37:43.14 CBV.dial SSV @16:37:43.92 Allowing 130s for 25.0ml of remaining 1000.0ml @16:37:43.94 Intake.select :open @16:37:46.11 Intake @15.1psia, vacuum from 9.6 to 12.6psi @16:37:46.12 SS.reconfigure :inhaling 2.20ml/s @16:37:46.17 SS.seek 25ml @16:37:54.04 Vacuum too high -- waiting up to 40 seconds @16:37:54.06 SS.reconfigure :exhale @16:37:54.11 SS.hold @16:38:02.06 Intake @18.3psia, vacuum from 10.0 to 13.0psi @16:38:02.08 SS.reconfigure :inhaling 1.76ml/s @16:38:02.13 SS.seek 25ml @16:38:05.10 Vacuum too high -- waiting up to 40 seconds @16:38:05.12 SS.reconfigure :exhale @16:38:05.17 SS.hold @16:38:07.88 Intake @18.7psia, vacuum from 10.0 to 13.0psi @16:38:07.90 SS.reconfigure :inhaling 1.41ml/s @16:38:07.95 SS.seek 25ml @16:38:10.39 Vacuum too high -- waiting up to 40 seconds @16:38:10.41 SS.reconfigure :exhale @16:38:10.46 SS.hold @16:38:12.63 Intake @18.9psia, vacuum from 10.0 to 13.0psi @16:38:12.65 SS.reconfigure :inhaling 1.13ml/s @16:38:13.15 SS.seek 25ml @16:38:15.98 Vacuum too high -- waiting up to 40 seconds @16:38:16.00 SS.reconfigure :exhale @16:38:16.05 SS.hold @16:38:18.24 Intake @18.8psia, vacuum from 10.0 to 13.0psi @16:38:18.26 SS.reconfigure :inhaling 0.91ml/s @16:38:18.30 SS.seek 25ml @16:38:22.10 Vacuum too high -- waiting up to 40 seconds @16:38:22.12 SS.reconfigure :exhale @16:38:22.17 SS.hold @16:38:24.37 Intake @18.9psia, vacuum from 10.0 to 13.0psi @16:38:24.41 SS.reconfigure :inhaling 0.73ml/s @16:38:24.48 SS.seek 25ml @16:38:28.73 Stroke completed -- waiting for pressure to equalize ... @16:38:28.75 SS.reconfigure :exhale @16:38:28.79 SS.hold @16:38:53.64 Intake.select :close @16:38:53.79 SSV.dial :exhaust @16:38:54.55 Exhaust.select :open @16:38:54.67 SS.seek 0ml @16:39:03.50 Exhaust.select :close @16:39:03.61 SSV.dial CBV @16:39:03.65 CBV.dial SSV @16:39:04.42 Allowing 130s for 25.0ml of remaining 975.0ml @16:39:04.44 Intake.select :open @16:39:06.60 Intake @15.6psia, vacuum from 10.0 to 13.0psi @16:39:06.62 SS.reconfigure :inhaling 0.73ml/s @16:39:06.67 SS.seek 25ml @16:39:43.51 Stroke completed -- waiting for pressure to equalize ... @16:39:43.53 SS.reconfigure :exhale @16:39:43.57 SS.hold @16:40:12.46 Intake.select :close @16:40:12.62 SSV.dial :exhaust @16:40:13.39 Exhaust.select :open @16:40:13.51 SS.seek 0ml @16:40:22.31 Exhaust.select :close @16:40:22.87 SSV.dial CBV @16:40:22.89 CBV.dial SSV @16:40:23.69 Allowing 130s for 25.0ml of remaining 950.0ml @16:40:23.70 Intake.select :open @16:40:25.87 Intake @15.6psia, vacuum from 10.0 to 13.0psi @16:40:25.89 SS.reconfigure :inhaling 0.73ml/s @16:40:25.94 SS.seek 25ml @16:40:46.75 recoverPRVreagent08 @16:40:46.76 PTV.dial :mixing @16:40:47.52 PBV.dial :waste1 @16:40:47.90 delay 30 seconds @16:41:02.37 Stroke completed -- waiting for pressure to equalize ... @16:41:02.38 SS.reconfigure :exhale @16:41:02.43 SS.hold @16:41:17.92 PBV.dial between PSV and waste1 @16:41:18.29 PSV.dial :air @16:41:19.21 PS.reconfigure :fast @16:41:19.29 PS.seek 2.5ml @16:41:27.45 Intake.select :close @16:41:27.61 SSV.dial :exhaust @16:41:28.37 Exhaust.select :open @16:41:28.49 SS.seek 0ml @16:41:37.34 Exhaust.select :close @16:41:37.45 SSV.dial CBV @16:41:37.49 CBV.dial SSV @16:41:38.28 Allowing 130s for 25.0ml of remaining 925.0ml @16:41:38.30 Intake.select :open @16:41:40.46 Intake @15.7psia, vacuum from 10.0 to 13.0psi @16:41:40.48 SS.reconfigure :inhaling 0.73ml/s @16:41:40.53 SS.seek 25ml @16:41:43.46 PSV.dial :mixloop @16:41:43.94 delay 2 seconds @16:41:45.96 PSV.dial :air @16:41:46.43 delay 2 seconds @16:41:48.45 PSV.dial :mixloop @16:41:48.91 delay 2 seconds @16:41:50.93 PSV.dial :air @16:41:51.40 PS.seek 0ml @16:42:14.86 washCycleDA #1 of 2 @16:42:14.88 PSR.select :air @16:42:14.89 PSV.dial PSR @16:42:16.24 Pulling 0.5ml of PSR.wash @16:42:16.25 PSR.select :wash @16:42:16.36 PS.reconfigure :default @16:42:16.41 PS.seek 0.5ml @16:42:16.90 Stroke completed -- waiting for pressure to equalize ... @16:42:16.91 SS.reconfigure :exhale @16:42:16.96 SS.hold @16:42:22.33 delay 5 seconds @16:42:27.35 PSR.select :air @16:42:27.46 PS.seek 2ml @16:42:41.48 Intake.select :close @16:42:41.64 SSV.dial :exhaust @16:42:42.41 Exhaust.select :open @16:42:42.53 SS.seek 0ml @16:42:43.64 PTV.dial :puck @16:42:44.39 PSV.dial PTV @16:42:45.16 PBV.dial :waste1 @16:42:45.60 PS.seek 1.5ml @16:42:51.53 delay 3 seconds @16:42:51.56 Exhaust.select :close @16:42:51.67 SSV.dial CBV @16:42:51.70 CBV.dial SSV @16:42:52.47 Allowing 130s for 25.0ml of remaining 900.0ml @16:42:52.48 Intake.select :open @16:42:54.54 PBV.dial between PSV and waste1 @16:42:54.65 Intake @15.5psia, vacuum from 10.0 to 13.0psi @16:42:55.13 SS.reconfigure :inhaling 0.73ml/s @16:42:55.19 SS.seek 25ml @16:42:55.22 PS.seek 0ml @16:43:11.37 delay 2:00 @16:43:31.17 Stroke completed -- waiting for pressure to equalize ... @16:43:31.18 SS.reconfigure :exhale @16:43:31.23 SS.hold @16:43:53.70 Intake.select :close @16:43:54.24 SSV.dial :exhaust @16:43:55.07 Exhaust.select :open @16:43:55.19 SS.seek 0ml @16:44:03.96 Exhaust.select :close @16:44:04.07 SSV.dial CBV @16:44:04.11 CBV.dial SSV @16:44:04.87 Allowing 130s for 25.0ml of remaining 875.0ml @16:44:04.89 Intake.select :open @16:44:07.05 Intake @15.6psia, vacuum from 10.0 to 13.0psi @16:44:07.07 SS.reconfigure :inhaling 0.73ml/s @16:44:07.12 SS.seek 25ml @16:44:43.60 Stroke completed -- waiting for pressure to equalize ... @16:44:43.61 SS.reconfigure :exhale @16:44:43.66 SS.hold @16:45:06.15 Intake.select :close @16:45:06.31 SSV.dial :exhaust @16:45:07.07 Exhaust.select :open @16:45:07.19 SS.seek 0ml @16:45:11.38 recoverPSRreagent08 @16:45:11.40 PTV.dial :puck @16:45:11.47 PBV.dial :waste1 @16:45:11.85 delay 30 seconds @16:45:16.24 Exhaust.select :close @16:45:16.35 SSV.dial CBV @16:45:16.37 CBV.dial SSV @16:45:17.15 Allowing 130s for 25.0ml of remaining 850.0ml @16:45:17.16 Intake.select :open @16:45:19.33 Intake @15.7psia, vacuum from 10.0 to 13.0psi @16:45:19.35 SS.reconfigure :inhaling 0.73ml/s @16:45:19.40 SS.seek 25ml @16:45:41.87 PBV.dial between PSV and waste1 @16:45:42.24 PSR.select :air @16:45:42.25 PSV.dial PSR @16:45:43.04 PS.reconfigure :fast @16:45:43.09 PS.seek 2.5ml @16:45:55.19 Stroke completed -- waiting for pressure to equalize ... @16:45:55.21 SS.reconfigure :exhale @16:45:55.25 SS.hold @16:46:07.14 PSV.dial PTV @16:46:07.96 delay 5 seconds @16:46:12.99 PSV.dial PSR @16:46:13.75 delay 5 seconds @16:46:18.77 PSV.dial PTV @16:46:19.53 delay 5 seconds @16:46:19.77 Intake.select :close @16:46:19.93 SSV.dial :exhaust @16:46:20.70 Exhaust.select :open @16:46:20.81 SS.seek 0ml @16:46:24.55 PSV.dial :air @16:46:25.32 PS.seek 0ml @16:46:29.84 Exhaust.select :close @16:46:29.95 SSV.dial CBV @16:46:29.97 CBV.dial SSV @16:46:30.76 Allowing 130s for 25.0ml of remaining 825.0ml @16:46:30.77 Intake.select :open @16:46:32.94 Intake @15.5psia, vacuum from 10.0 to 13.0psi @16:46:32.96 SS.reconfigure :inhaling 0.73ml/s @16:46:33.00 SS.seek 25ml @16:46:48.99 washCycleDA #2 of 2 @16:46:49.00 PSR.select :air @16:46:49.02 PSV.dial PSR @16:46:50.39 Pulling 0.5ml of PSR.wash @16:46:50.40 PSR.select :wash @16:46:50.52 PS.reconfigure :default @16:46:50.57 PS.seek 0.5ml @16:46:56.45 delay 5 seconds @16:47:01.47 PSR.select :air @16:47:01.59 PS.seek 2ml @16:47:06.91 Vacuum too high -- waiting up to 40 seconds @16:47:06.93 SS.reconfigure :exhale @16:47:07.37 SS.hold @16:47:17.76 PTV.dial :puck @16:47:17.84 PSV.dial PTV @16:47:18.68 PBV.dial :waste1 @16:47:18.76 Intake @17.1psia, vacuum from 10.0 to 13.0psi @16:47:18.78 SS.reconfigure :inhaling 0.59ml/s @16:47:18.83 SS.seek 25ml @16:47:19.12 PS.seek 1.5ml @16:47:21.51 Stroke completed -- waiting for pressure to equalize ... @16:47:21.52 SS.reconfigure :exhale @16:47:21.56 SS.hold @16:47:25.11 delay 3 seconds @16:47:28.13 PBV.dial between PSV and waste1 @16:47:28.50 PS.seek 0ml @16:47:34.49 Intake.select :close @16:47:34.65 SSV.dial :exhaust @16:47:35.41 Exhaust.select :open @16:47:35.53 SS.seek 0ml @16:47:44.32 Exhaust.select :close @16:47:44.43 SSV.dial CBV @16:47:44.47 CBV.dial SSV @16:47:44.86 delay 2:00 @16:47:45.23 Allowing 130s for 25.0ml of remaining 800.0ml @16:47:45.24 Intake.select :open @16:47:47.41 Intake @15.6psia, vacuum from 10.0 to 13.0psi @16:47:47.43 SS.reconfigure :inhaling 0.59ml/s @16:47:47.48 SS.seek 25ml @16:48:32.22 Stroke completed -- waiting for pressure to equalize ... @16:48:32.23 SS.reconfigure :exhale @16:48:32.28 SS.hold @16:48:52.93 Intake.select :close @16:48:53.09 SSV.dial :exhaust @16:48:53.87 Exhaust.select :open @16:48:53.99 SS.seek 0ml @16:49:03.10 Exhaust.select :close @16:49:03.22 SSV.dial CBV @16:49:03.24 CBV.dial SSV @16:49:04.02 Allowing 130s for 25.0ml of remaining 775.0ml @16:49:04.04 Intake.select :open @16:49:06.20 Intake @15.7psia, vacuum from 10.0 to 13.0psi @16:49:06.22 SS.reconfigure :inhaling 0.59ml/s @16:49:06.26 SS.seek 25ml @16:49:44.88 recoverPSRreagent08 @16:49:44.90 PTV.dial :puck @16:49:44.97 PBV.dial :waste1 @16:49:45.80 delay 30 seconds @16:49:50.57 Stroke completed -- waiting for pressure to equalize ... @16:49:50.59 SS.reconfigure :exhale @16:49:50.63 SS.hold @16:50:15.81 PBV.dial between PSV and waste1 @16:50:16.18 PSR.select :air @16:50:16.20 PSV.dial PSR @16:50:17.36 PS.reconfigure :fast @16:50:17.48 PS.seek 2.5ml @16:50:17.64 Intake.select :close @16:50:17.80 SSV.dial :exhaust @16:50:18.57 Exhaust.select :open @16:50:18.69 SS.seek 0ml @16:50:27.77 Exhaust.select :close @16:50:27.88 SSV.dial CBV @16:50:27.91 CBV.dial SSV @16:50:28.68 Allowing 130s for 25.0ml of remaining 750.0ml @16:50:28.69 Intake.select :open @16:50:30.86 Intake @15.4psia, vacuum from 9.9 to 12.9psi @16:50:30.88 SS.reconfigure :inhaling 0.59ml/s @16:50:30.93 SS.seek 25ml @16:50:41.67 PSV.dial PTV @16:50:42.43 delay 5 seconds @16:50:47.46 PSV.dial PSR @16:50:48.22 delay 5 seconds @16:50:53.24 PSV.dial PTV @16:50:54.12 delay 5 seconds @16:50:59.14 PSV.dial :air @16:50:59.92 PS.seek 0ml @16:51:15.13 Stroke completed -- waiting for pressure to equalize ... @16:51:15.14 SS.reconfigure :exhale @16:51:15.19 SS.hold @16:51:23.61 getPMLReagent08 @16:51:23.62 PRV.dial :air @16:51:24.07 delay 5 seconds @16:51:29.09 PTV.dial :PRVmixing @16:51:30.42 delay 5 seconds @16:51:35.44 PRV.dial :ab2 @16:51:35.90 PTV.dial :PRVmixing @16:51:35.98 PSV.dial :mixloop @16:51:36.46 PS.reconfigure :default @16:51:36.51 PS.seek 0.3ml @16:51:40.35 delay 2 seconds @16:51:42.37 PRV.dial :air @16:51:42.90 PS.seek 0.55ml @16:51:45.92 Intake.select :close @16:51:46.07 SSV.dial :exhaust @16:51:46.29 addPMLReagent08 @16:51:46.30 PSV.dial :air @16:51:46.85 Exhaust.select :open @16:51:46.93 PS.seek 2ml @16:51:47.00 SS.seek 0ml @16:51:56.00 Exhaust.select :close @16:51:56.11 SSV.dial CBV @16:51:56.15 CBV.dial SSV @16:51:56.91 Allowing 130s for 25.0ml of remaining 725.0ml @16:51:56.92 Intake.select :open @16:51:59.09 Intake @15.5psia, vacuum from 10.0 to 13.0psi @16:51:59.11 SS.reconfigure :inhaling 0.59ml/s @16:51:59.62 SS.seek 25ml @16:52:02.63 PSV.dial :PML @16:52:03.09 PTV.dial :mixing @16:52:03.85 PBV.dial :waste1 @16:52:04.29 PS.seek 1.85ml @16:52:06.70 PBV.dial between PSV and waste1 @16:52:07.08 PS.seek 0ml @16:52:26.98 delay 2 seconds @16:52:29.00 delay 8:00 @16:52:40.53 Vacuum too high -- waiting up to 40 seconds @16:52:40.54 SS.reconfigure :exhale @16:52:40.60 SS.hold @16:52:57.04 Intake @15.7psia, vacuum from 10.0 to 13.0psi @16:52:57.06 SS.reconfigure :inhaling 0.47ml/s @16:52:57.58 SS.seek 25ml @16:53:02.29 Stroke completed -- waiting for pressure to equalize ... @16:53:02.30 SS.reconfigure :exhale @16:53:02.35 SS.hold @16:53:20.72 Intake.select :close @16:53:20.88 SSV.dial :exhaust @16:53:21.64 Exhaust.select :open @16:53:21.76 SS.seek 0ml @16:53:30.56 Exhaust.select :close @16:53:30.68 SSV.dial CBV @16:53:30.72 CBV.dial SSV @16:53:31.45 Allowing 130s for 25.0ml of remaining 700.0ml @16:53:31.47 Intake.select :open @16:53:33.64 Intake @15.3psia, vacuum from 9.8 to 12.8psi @16:53:33.66 SS.reconfigure :inhaling 0.47ml/s @16:53:33.71 SS.seek 25ml @16:54:29.23 Stroke completed -- waiting for pressure to equalize ... @16:54:29.24 SS.reconfigure :exhale @16:54:29.29 SS.hold @16:55:00.42 Intake.select :close @16:55:00.58 SSV.dial :exhaust @16:55:01.34 Exhaust.select :open @16:55:01.46 SS.seek 0ml @16:55:10.53 Exhaust.select :close @16:55:10.65 SSV.dial CBV @16:55:10.67 CBV.dial SSV @16:55:11.44 Allowing 130s for 25.0ml of remaining 675.0ml @16:55:11.45 Intake.select :open @16:55:13.62 Intake @15.4psia, vacuum from 9.9 to 12.9psi @16:55:13.64 SS.reconfigure :inhaling 0.47ml/s @16:55:13.69 SS.seek 25ml @16:56:08.90 Stroke completed -- waiting for pressure to equalize ... @16:56:08.91 SS.reconfigure :exhale @16:56:08.96 SS.hold @16:56:49.15 Intake.select :close @16:56:49.31 SSV.dial :exhaust @16:56:50.09 Exhaust.select :open @16:56:50.21 SS.seek 0ml @16:56:59.09 Exhaust.select :close @16:56:59.20 SSV.dial CBV @16:56:59.24 CBV.dial SSV @16:57:00.01 Allowing 130s for 25.0ml of remaining 650.0ml @16:57:00.03 Intake.select :open @16:57:02.19 Intake @15.3psia, vacuum from 9.8 to 12.8psi @16:57:02.21 SS.reconfigure :inhaling 0.47ml/s @16:57:02.26 SS.seek 25ml @16:57:45.14 Vacuum too high -- waiting up to 40 seconds @16:57:45.16 SS.reconfigure :exhale @16:57:45.21 SS.hold @16:58:06.06 Intake @17.0psia, vacuum from 10.0 to 13.0psi @16:58:06.08 SS.reconfigure :inhaling 0.38ml/s @16:58:06.13 SS.seek 25ml @16:58:22.32 Stroke completed -- waiting for pressure to equalize ... @16:58:22.33 SS.reconfigure :exhale @16:58:22.38 SS.hold @16:58:49.17 Intake.select :close @16:58:49.33 SSV.dial :exhaust @16:58:50.11 Exhaust.select :open @16:58:50.23 SS.seek 0ml @16:58:59.35 Exhaust.select :close @16:58:59.47 SSV.dial CBV @16:58:59.49 CBV.dial SSV @16:59:00.26 Allowing 130s for 25.0ml of remaining 625.0ml @16:59:00.29 Intake.select :open @16:59:02.45 Intake @15.4psia, vacuum from 9.9 to 12.9psi @16:59:02.47 SS.reconfigure :inhaling 0.38ml/s @16:59:02.99 SS.seek 25ml @17:00:11.66 Stroke completed -- waiting for pressure to equalize ... @17:00:11.67 SS.reconfigure :exhale @17:00:11.73 SS.hold @17:00:29.02 recoverPRVreagent08 @17:00:29.03 PTV.dial :mixing @17:00:29.09 PBV.dial :waste1 @17:00:29.47 delay 30 seconds @17:00:51.95 SS.reconfigure :default @17:00:52.00 SS.hold @17:00:52.07 Intake.select :close @17:00:52.23 ShallowSampler::Clogged in WCR -- Intake @15psia & Outlet @14psia won't equalize after 400.0ml @17:00:52.24 SSV.dial :exhaust @17:00:53.02 Exhaust.select :open @17:00:53.14 SS.reconfigure :exhale @17:00:53.19 SS.seek 0ml @17:00:59.49 PBV.dial between PSV and waste1 @17:00:59.86 PSV.dial :air @17:01:00.33 PS.reconfigure :fast @17:01:00.38 PS.seek 2.5ml @17:01:02.26 Exhaust.select :close @17:01:02.37 Sampled 400.0ml @17:01:02.39 Drying puck before fixing for 5:00 @17:01:02.40 CBV.dial :waste2b @17:01:03.16 delay 1:00 @17:01:24.53 PSV.dial :mixloop @17:01:25.00 delay 2 seconds @17:01:27.02 PSV.dial :air @17:01:27.49 delay 2 seconds @17:01:29.50 PSV.dial :mixloop @17:01:29.97 delay 2 seconds @17:01:31.99 PSV.dial :air @17:01:32.60 PS.seek 0ml @17:01:56.13 washCycleDA #1 of 3 @17:01:56.14 PSR.select :air @17:01:56.15 PSV.dial PSR @17:01:57.50 Pulling 0.5ml of PSR.wash @17:01:57.51 PSR.select :wash @17:01:57.62 PS.reconfigure :default @17:01:57.67 PS.seek 0.5ml @17:02:03.18 CSV.dial :air @17:02:03.63 CS.reconfigure :fast @17:02:03.71 CS.seek 10ml @17:02:03.73 delay 5 seconds @17:02:08.75 PSR.select :air @17:02:08.87 PS.seek 2ml @17:02:25.06 PTV.dial :puck @17:02:25.81 PSV.dial PTV @17:02:27.03 PBV.dial :waste1 @17:02:27.48 PS.seek 1.5ml @17:02:28.47 Pumping wcr puck from tube 4 up to 35psi... @17:02:28.49 CBV.dial SSV @17:02:29.76 CBV.dial between CSV and waste2b @17:02:30.74 CSV.dial between air and CTV @17:02:31.07 CS.seek 7.383ml @17:02:33.44 delay 3 seconds @17:02:36.46 PBV.dial between PSV and waste1 @17:02:36.82 PS.seek 0ml @17:02:37.88 CTV.dial CSV,avoiding: :air @17:02:37.91 CSV.dial CTV @17:02:38.74 CS.reconfigure :pressurize @17:02:38.79 CS.seek 0ml @17:02:52.97 delay 2:00 @17:02:57.46 CBV.dial :waste2b @17:02:57.80 CS.reconfigure :default @17:02:57.85 CS.seek 0ml @17:02:59.56 delay 1:00 @17:03:59.59 CTV.dial :air,avoiding: :intake @17:04:00.93 Fix #1 of 2 (with RNAlater) for 2:30 @17:04:00.94 CSV.dial CSR @17:04:01.39 Pulling 2ml of CSR.RNAlater @17:04:01.40 CSR.select :RNAlater @17:04:01.52 CS.seek 2ml @17:04:07.44 delay 5 seconds @17:04:12.46 CSR.select :air @17:04:12.58 CS.reconfigure :fast @17:04:12.63 CS.seek 10ml @17:04:32.47 Pumping wcr puck from tube 4 up to 35psi... @17:04:32.49 CBV.dial SSV @17:04:33.76 CBV.dial between CSV and waste2b @17:04:34.73 CSV.dial between air and CTV @17:04:35.33 CS.seek 7.269ml @17:04:42.44 CTV.dial CSV,avoiding: :air @17:04:42.47 CSV.dial CTV @17:04:43.85 CS.reconfigure :pressurize @17:04:43.90 CS.seek 0ml @17:04:52.99 recoverPSRreagent08 @17:04:53.01 PTV.dial :puck @17:04:53.25 PBV.dial :waste1 @17:04:53.61 delay 30 seconds @17:05:02.96 delay 2:30 @17:05:23.62 PBV.dial between PSV and waste1 @17:05:23.98 PSR.select :air @17:05:24.00 PSV.dial PSR @17:05:24.77 PS.reconfigure :fast @17:05:24.82 PS.seek 2.5ml @17:05:48.90 PSV.dial PTV @17:05:49.79 delay 5 seconds @17:05:54.81 PSV.dial PSR @17:05:55.57 delay 5 seconds @17:06:00.60 PSV.dial PTV @17:06:01.36 delay 5 seconds @17:06:06.38 PSV.dial :air @17:06:07.14 PS.seek 0ml @17:06:30.53 washCycleDA #2 of 3 @17:06:30.54 PSR.select :air @17:06:30.56 PSV.dial PSR @17:06:31.92 Pulling 0.5ml of PSR.wash @17:06:31.93 PSR.select :wash @17:06:32.05 PS.reconfigure :default @17:06:32.10 PS.seek 0.5ml @17:06:38.03 delay 5 seconds @17:06:43.05 PSR.select :air @17:06:43.17 PS.seek 2ml @17:06:59.37 PTV.dial :puck @17:06:59.45 PSV.dial PTV @17:07:00.21 PBV.dial :waste1 @17:07:00.65 PS.seek 1.5ml @17:07:06.62 delay 3 seconds @17:07:09.64 PBV.dial between PSV and waste1 @17:07:10.01 PS.seek 0ml @17:07:26.31 delay 2:00 @17:07:32.98 CBV.dial :waste2b @17:07:33.31 CS.reconfigure :default @17:07:33.36 CS.seek 0ml @17:07:34.75 delay 1:00 @17:08:34.77 CTV.dial :air,avoiding: :intake @17:08:36.09 Fix #2 of 2 (with RNAlater) for 2:30 @17:08:36.11 CSV.dial CSR @17:08:36.54 Pulling 2ml of CSR.RNAlater @17:08:36.55 CSR.select :RNAlater @17:08:36.67 CS.seek 2ml @17:08:42.68 delay 5 seconds @17:08:47.70 CSR.select :air @17:08:47.82 CS.reconfigure :fast @17:08:47.88 CS.seek 10ml @17:09:07.72 Pumping wcr puck from tube 4 up to 35psi... @17:09:07.73 CBV.dial SSV @17:09:09.00 CBV.dial between CSV and waste2b @17:09:09.98 CSV.dial between air and CTV @17:09:11.17 CS.seek 7.286ml @17:09:18.25 CTV.dial CSV,avoiding: :air @17:09:18.27 CSV.dial CTV @17:09:19.67 CS.reconfigure :pressurize @17:09:19.72 CS.seek 0ml @17:09:26.33 recoverPSRreagent08 @17:09:26.34 PTV.dial :puck @17:09:26.35 PBV.dial :waste1 @17:09:26.73 delay 30 seconds @17:09:36.36 delay 2:30 @17:09:56.74 PBV.dial between PSV and waste1 @17:09:57.11 PSR.select :air @17:09:57.13 PSV.dial PSR @17:09:57.90 PS.reconfigure :fast @17:09:57.95 PS.seek 2.5ml @17:10:21.93 PSV.dial PTV @17:10:22.82 delay 5 seconds @17:10:27.84 PSV.dial PSR @17:10:28.60 delay 5 seconds @17:10:33.62 PSV.dial PTV @17:10:34.40 delay 5 seconds @17:10:39.42 PSV.dial :air @17:10:40.18 PS.seek 0ml @17:11:03.57 washCycleDA #3 of 3 @17:11:03.58 PSR.select :air @17:11:03.60 PSV.dial PSR @17:11:04.97 Pulling 0.5ml of PSR.wash @17:11:04.98 PSR.select :wash @17:11:05.10 PS.reconfigure :default @17:11:05.15 PS.seek 0.5ml @17:11:11.18 delay 5 seconds @17:11:16.20 PSR.select :air @17:11:16.32 PS.seek 2ml @17:11:32.45 PTV.dial :puck @17:11:32.47 PSV.dial PTV @17:11:33.26 PBV.dial :waste1 @17:11:33.69 PS.seek 1.5ml @17:11:39.64 delay 3 seconds @17:11:42.66 PBV.dial between PSV and waste1 @17:11:43.03 PS.seek 0ml @17:11:59.21 delay 2:00 @17:12:06.38 CBV.dial :waste2b @17:12:06.73 CS.reconfigure :default @17:12:06.78 CS.seek 0ml @17:12:09.96 delay 1:00 @17:13:09.99 CTV.dial :air,avoiding: :intake @17:13:59.23 recoverPSRreagent08 @17:13:59.25 PTV.dial :puck @17:13:59.26 PBV.dial :waste1 @17:13:59.63 delay 30 seconds @17:14:29.65 PBV.dial between PSV and waste1 @17:14:30.03 PSR.select :air @17:14:30.05 PSV.dial PSR @17:14:30.82 PS.reconfigure :fast @17:14:30.87 PS.seek 2.5ml @17:14:55.14 PSV.dial PTV @17:14:56.38 delay 5 seconds @17:15:01.40 PSV.dial PSR @17:15:02.17 delay 5 seconds @17:15:07.19 PSV.dial PTV @17:15:07.97 delay 5 seconds @17:15:12.99 PSV.dial :air @17:15:13.76 PS.seek 0ml @17:15:37.20 Gate.power :camera,:ON @17:15:37.21 Mixing DA Substrates @17:15:37.22 PSR.select :air @17:15:37.24 PSV.dial PSR @17:15:38.64 Pulling 0.25ml of PSR.sub1 @17:15:38.67 PSR.select :sub1 @17:15:38.85 PS.reconfigure :default @17:15:38.97 PS.seek 0.25ml @17:15:42.58 delay 5 seconds @17:15:47.61 Pulling 0.25ml of PSR.sub2 @17:15:47.62 PSR.select :sub2 @17:15:47.74 PS.seek 0.5ml @17:15:51.17 delay 5 seconds @17:15:56.19 PSR.select :air @17:15:56.30 PS.seek 2ml @17:16:12.51 PTV.dial :puck @17:16:12.53 PSV.dial PTV @17:16:13.28 PBV.dial :waste1 @17:16:13.71 PS.seek 1.5ml @17:16:19.73 delay 3 seconds @17:16:22.75 PBV.dial between PSV and waste1 @17:16:23.13 PS.seek 0ml @17:16:39.33 delay 10 seconds @17:16:49.35 recoverPSRreagent08 @17:16:49.36 PTV.dial :puck @17:16:49.38 PBV.dial :waste1 @17:16:49.76 delay 30 seconds @17:17:19.79 PBV.dial between PSV and waste1 @17:17:20.17 PSR.select :air @17:17:20.19 PSV.dial PSR @17:17:20.97 PS.reconfigure :fast @17:17:21.02 PS.seek 2.5ml @17:17:45.32 PSV.dial PTV @17:17:46.11 delay 5 seconds @17:17:51.13 PSV.dial PSR @17:17:51.89 delay 5 seconds @17:17:56.91 PSV.dial PTV @17:17:57.79 delay 5 seconds @17:18:02.81 PSV.dial :air @17:18:03.63 PS.seek 0ml @17:18:27.76 Additional air clear @17:18:27.77 PSR.select :air @17:18:27.79 PSV.dial PSR @17:18:29.17 Pulling 2ml of PSR.air @17:18:29.18 PSR.select :air @17:18:29.20 PS.reconfigure :default @17:18:29.25 PS.seek 2ml @17:18:50.54 delay 5 seconds @17:18:55.56 PTV.dial :puck @17:18:55.58 PSV.dial PTV @17:18:56.34 PBV.dial :waste1 @17:18:56.79 PS.seek 1.5ml @17:19:02.76 delay 3 seconds @17:19:05.78 PBV.dial between PSV and waste1 @17:19:06.17 delay 1 second @17:19:07.19 PSV.dial :waste1 @17:19:08.27 PS.seek 0ml @17:19:24.50 Processing Puck Heater at 28.1C before imaging @17:19:24.51 PH.coast @17:19:24.67 Imaging da puck in Processing Clamp while storing it to tube 3 @17:19:25.39 SC.reconfigure :tight @17:19:25.44 SC.seek 3 @17:19:29.05 SC.configure :limp @17:19:29.27 Elbow.reconfigure :tight @17:19:29.32 Elbow.seek 3 @17:19:31.02 Forearm.reconfigure :tight @17:19:31.07 Forearm.seek 3 + 450 counts @17:19:32.08 SE.reconfigure :pick @17:19:32.14 SE.seek :beamBreak @17:19:34.58 SE.configure :default @17:19:34.75 Elbow.reconfigure :limp @17:19:34.80 Forearm.reconfigure :loose @17:19:34.84 Forearm.seek 3 @17:19:35.10 Elbow.hold @17:19:35.17 Elbow.reconfigure :tight @17:19:35.27 SE.seek 11.12 Pucks below Top Plate @17:19:35.71 Forearm.force -20 @17:19:35.75 Forearm.seek 3 + 312 counts @17:19:36.03 Forearm.reconfigure :tight @17:19:36.08 Forearm.seek 3 + 450 counts @17:19:36.28 SE.seek 12.12 Pucks below Top Plate @17:19:36.92 Forearm.reconfigure :loose @17:19:36.97 Forearm.seek :retract @17:19:37.90 Elbow.seek PC @17:19:40.42 Elbow.reconfigure :loose @17:19:40.52 Forearm.seek PC @17:19:41.50 Hand.close @17:19:42.18 PC.seek :open @17:19:46.96 Forearm.seek :retract @17:19:47.93 Elbow.reconfigure :tight @17:19:47.98 Elbow.seek 3 @17:19:51.09 Forearm.reconfigure :tight @17:19:51.14 Forearm.seek 3 + 450 counts @17:19:52.11 Hand.open @17:19:52.74 SE.seek 11.77 Pucks below Top Plate @17:19:53.00 SE.reconfigure :seal @17:19:53.05 SE.seek 10.8 Pucks below Top Plate @17:19:53.62 Lights, camera, ACTION! @17:19:53.66 PuckCamera: snap -bin=2,2 -auto /var/log/esp/da23nov2817h575ml.tif @17:20:13.11 Waiting >20 seconds for da to relinquish FlushPuck @17:20:16.23 SX HX9/16 (USB:07): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 19.302 seconds /var/log/esp/da23nov2817h575ml.tif: TIFF Upload Complete @17:20:16.26 PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da23nov2817h575ml15s.tif @17:20:31.91 SX HX9/16 (USB:07): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15 seconds /var/log/esp/midres/da23nov2817h575ml15s.tif: TIFF Upload Complete @17:20:31.93 PuckCamera: snap -bin=2,2 20.0 /var/log/esp/midres/da23nov2817h575ml20s.tif @17:20:52.55 SX HX9/16 (USB:07): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 20 seconds /var/log/esp/midres/da23nov2817h575ml20s.tif: TIFF Upload Complete @17:20:52.56 Gate.power :camera,:OFF @17:20:52.58 DAfans.email "19.302 sec auto-exposure",Subject: "da23nov2817h575ml images!" @17:20:52.83 Elbow.seek 3 @17:20:53.22 Forearm.seek 3 + 450 counts @17:20:53.32 SE.reconfigure :gently @17:20:53.38 SE.seek 11.52 Pucks below Top Plate @17:20:54.04 Elbow.reconfigure :limp @17:20:54.10 Forearm.reconfigure :loose @17:20:54.15 Forearm.seek 3 @17:20:54.46 Elbow.hold @17:20:54.55 Elbow.reconfigure :tight @17:20:54.61 SE.reconfigure :default @17:20:54.69 SE.seek 11.7 Pucks below Top Plate @17:20:55.16 Forearm.force -20 @17:20:55.20 Forearm.seek 3 + 303 counts @17:20:55.49 Forearm.reconfigure :tight @17:20:55.54 Forearm.seek 3 + 450 counts @17:20:55.94 SE.seek :down @17:20:57.23 Forearm.reconfigure :loose @17:20:57.33 Forearm.seek :retract @17:20:58.66 SE.stop @17:20:58.92 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @17:20:59.42 Elbow.seek FlushPuck::Garage @17:21:01.33 Elbow.reconfigure :loose @17:21:01.60 Forearm.seek :garage @17:21:02.63 Hand.close @17:21:03.56 Forearm.seek :retract @17:21:04.58 Elbow.reconfigure :tight @17:21:04.68 Elbow.seek PC @17:21:06.03 Elbow.reconfigure :loose @17:21:06.23 Forearm.reconfigure :tight @17:21:06.33 Forearm.seek PC @17:21:07.90 PC.seek :closed @17:21:12.65 Processing Clamp onSpring/offSpring for 16/26 samples, on/off torque 1.63 @17:21:12.82 Hand.open @17:21:13.64 Forearm.reconfigure :loose @17:21:13.75 Forearm.seek :retract @17:21:14.77 PSR.select :air @17:21:14.84 Storing wcr puck in Collection Clamp to tube 3 @17:21:14.87 PSV.dial PSR @17:21:15.36 SC.reconfigure :tight @17:21:15.45 SC.seek 3 @17:21:15.57 Pulling 0.5ml of PSR.flush @17:21:15.62 PSR.select :flush @17:21:15.87 SC.configure :limp @17:21:16.18 PS.seek 0.5ml @17:21:17.50 Elbow.reconfigure :tight @17:21:17.60 Elbow.seek 3 @17:21:20.53 Forearm.reconfigure :tight @17:21:20.63 Forearm.seek 3 + 450 counts @17:21:21.85 SE.reconfigure :pick @17:21:21.97 SE.seek :beamBreak @17:21:23.16 delay 5 seconds @17:21:24.46 SE.configure :default @17:21:24.91 Elbow.reconfigure :limp @17:21:25.08 Forearm.reconfigure :loose @17:21:25.18 Forearm.seek 3 @17:21:25.48 Elbow.hold @17:21:25.61 Elbow.reconfigure :tight @17:21:25.82 SE.seek 12.14 Pucks below Top Plate @17:21:26.44 Forearm.force -20 @17:21:26.53 Forearm.seek 3 + 296 counts @17:21:27.06 Forearm.reconfigure :tight @17:21:27.16 Forearm.seek 3 + 450 counts @17:21:27.43 SE.seek 13.14 Pucks below Top Plate @17:21:28.24 Forearm.reconfigure :loose @17:21:28.36 Forearm.seek :retract @17:21:28.47 PSR.select :air @17:21:28.65 PS.seek 2.5ml @17:21:29.36 Elbow.seek CC @17:21:29.68 upsync @17:21:31.05 Elbow.reconfigure :loose @17:21:31.15 Forearm.seek CC @17:21:32.14 Hand.close @17:21:32.81 CC.seek :open @17:21:38.03 Forearm.seek :retract @17:21:39.02 Elbow.reconfigure :tight @17:21:39.08 Elbow.seek 3 @17:21:40.88 Elbow.reconfigure :limp @17:21:40.93 Forearm.seek 3 @17:21:41.99 Elbow.hold @17:21:42.06 Elbow.reconfigure :tight @17:21:42.11 Hand.open @17:21:42.81 Forearm.force -20 @17:21:42.86 Forearm.seek 3 + 313 counts @17:21:43.17 Forearm.reconfigure :tight @17:21:43.23 Forearm.seek 3 + 450 counts @17:21:43.48 SE.seek :down @17:21:44.61 Forearm.reconfigure :loose @17:21:44.66 Forearm.seek :retract @17:21:45.33 SE.stop @17:21:45.60
Starting finalPurge @17:21:49.98 delay 1 second @17:21:51.00 PRV.dial :waste2,avoiding: :ab2 @17:21:52.88 PTV.dial :PRVmixing @17:21:54.21 PSV.dial :mixloop @17:21:55.27 PS.seek :empty @17:22:05.38
Waiting >20 seconds for psFlush to relinquish FlushPuck @17:22:21.69 PSR.select :air @17:22:21.71 PSV.dial PSR @17:22:22.77 Pulling 0.5ml of PSR.flush @17:22:22.79 PSR.select :flush @17:22:22.91 PS.seek 0.5ml @17:22:28.88 delay 5 seconds @17:22:33.90 PSR.select :air @17:22:34.02 PS.seek 2.5ml @17:22:55.26 delay 1 second @17:22:56.27 PBV.dial :waste1 @17:22:57.08 PTV.dial :mixing @17:22:57.85 PSV.dial :mixloop @17:22:58.92 PS.seek :empty @17:23:25.19 PSV.dial PSR @17:23:26.36 PSR.select :flush @17:23:26.49 PS.seek 1ml @17:23:37.60 PSR.select :air @17:23:37.72 PS.seek 2.5ml @17:23:53.91 PSV.dial PTV @17:23:54.66 PTV.dial :puck @17:23:55.39 PBV.dial :waste1 @17:23:55.48 PS.seek 0ml @17:24:21.85 PSV.dial PSR @17:24:22.61 PSR.select :flush @17:24:22.73 PS.seek 0.5ml @17:24:28.66 PSR.select :air @17:24:28.78 PS.seek 2.5ml @17:24:50.03 PSV.dial PTV @17:24:50.80 PBV.dial PSV @17:24:51.37 PS.seek 0ml @17:25:17.75 PSV.dial PBV @17:25:18.23 PS.seek 2.5ml @17:25:44.53 PSV.dial :waste1 @17:25:45.29 PS.seek 0ml @17:26:11.62 PSV.dial PSR @17:26:12.09 PSR.select :air @17:26:12.12 PS.seek 2.5ml @17:26:38.47 PSV.dial PTV @17:26:39.20 PBV.dial PSV @17:26:39.29 PS.seek 0ml @17:27:05.66 PSV.dial PBV @17:27:06.13 PS.seek 2.5ml @17:27:32.47 PSV.dial :waste1 @17:27:33.21 PS.seek 0ml @17:27:59.59 PSV.dial PSR @17:28:00.06 PSR.select :flush @17:28:00.18 PS.seek 1ml @17:28:11.18 PSR.select :air @17:28:11.30 PS.seek 2.5ml @17:28:27.37 PSV.dial PTV @17:28:28.23 PTV.dial :puck @17:28:28.31 PBV.dial :waste1 @17:28:28.88 PS.seek 0ml @17:28:55.68 PSV.dial PSR @17:28:56.44 PSR.select :flush @17:28:56.56 PS.seek 0.5ml @17:29:02.81 PSR.select :air @17:29:02.92 PS.seek 2.5ml @17:29:24.22 PSV.dial PTV @17:29:24.98 PBV.dial PSV @17:29:25.55 PS.seek 0ml @17:29:52.09 PSV.dial PBV @17:29:52.59 PS.seek 2.5ml @17:30:18.90 PSV.dial :waste1 @17:30:19.64 PS.seek 0ml @17:30:46.03 PSV.dial PSR @17:30:46.50 PSR.select :air @17:30:46.52 PS.seek 2.5ml @17:31:12.84 PSV.dial PTV @17:31:13.57 PBV.dial PSV @17:31:13.77 PS.seek 0ml @17:31:40.27 PSV.dial PBV @17:31:40.75 PS.seek 2.5ml @17:32:07.15 PSV.dial :waste1 @17:32:07.89 PS.seek 0ml @17:32:34.31
Loading FlushPuck into Collection Clamp from Processing Clamp @17:32:34.50 Elbow.seek PC @17:32:37.01 Elbow.reconfigure :loose @17:32:37.11 Forearm.seek PC @17:32:38.07 Hand.close @17:32:38.67 PC.seek :open @17:32:43.42 Forearm.seek :retract @17:32:44.39 Elbow.reconfigure :tight @17:32:44.44 Elbow.seek CC @17:32:48.05 Elbow.reconfigure :loose @17:32:48.15 Forearm.reconfigure :tight @17:32:48.20 Forearm.seek CC @17:32:49.93 CC.seek :closed @17:32:54.47 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.40 @17:32:54.54 Hand.open @17:32:55.23 Forearm.reconfigure :loose @17:32:55.28 Forearm.seek :retract @17:32:56.20 Flushing the Collection Valve::Manifold @17:32:56.33 CSV.dial CSR @17:32:56.78 Pulling 5ml of CSR.flush @17:32:56.79 CSR.select :flush @17:32:56.91 CS.seek 5ml @17:33:10.51 delay 5 seconds @17:33:15.53 CSR.select :air @17:33:15.69 CS.reconfigure :fast @17:33:15.74 CS.seek 7.5ml @17:33:22.50 CSV.dial :waste @17:33:23.91 CS.reconfigure :default @17:33:23.96 CS.seek 0ml @17:33:44.19 Flushing lower manifold's waste1 port @17:33:44.32 CSV.dial :flush @17:33:44.79 CS.reconfigure :fast @17:33:44.83 CS.seek 5ml @17:33:57.53 delay 5 seconds @17:34:02.55 CSV.dial :air @17:34:03.06 CS.seek 7.001ml @17:34:08.53 CSV.dial CTV @17:34:08.55
CTV.dial CSV @17:34:09.92 CBV.dial :waste1 @17:34:10.69 CS.seek 0ml @17:34:27.22 Flushing lower manifold's waste2 port @17:34:27.34 CSV.dial :flush @17:34:28.08 CS.seek 5ml @17:34:40.78 delay 5 seconds @17:34:45.80 CSV.dial :air @17:34:46.31 CS.seek 7ml @17:34:52.03 CSV.dial CTV @17:34:52.05
CTV.dial CSV @17:34:52.51 CBV.dial :waste2 @17:34:54.02 CS.seek 0ml @17:35:10.56 Flushing lower manifold's waste2b port @17:35:10.69 CSV.dial :flush @17:35:11.43 CS.seek 5ml @17:35:24.09 delay 5 seconds @17:35:29.11 CSV.dial :air @17:35:29.64 CS.seek 7.001ml @17:35:35.22 CSV.dial CTV @17:35:35.24
CTV.dial CSV @17:35:35.72 CBV.dial :waste2b @17:35:36.77 CS.seek 0ml @17:35:53.40 Disinfecting external Intake with kill @17:35:53.53 CSV.dial CSR @17:35:54.09 Pulling 8ml of CSR.kill @17:35:54.10 CSR.select :kill @17:35:54.22 CS.seek 8ml @17:36:14.06 delay 5 seconds @17:36:19.08 CSR.select :air @17:36:19.20 CS.seek 10ml @17:36:24.72 CSV.dial CBV @17:36:24.76
CBV.dial CSV @17:36:25.81 CTV.dial :intake @17:36:26.59 Intake.select :open @17:36:26.70 CS.seek 2ml @17:36:45.62 Intake.select :close @17:36:45.73 CTV.dial :air @17:36:46.47 CS.reconfigure :default @17:36:46.52 CS.seek 0ml @17:36:52.68 CSV.dial CSR @17:36:53.70 Pulling 5ml of CSR.flush @17:36:53.71 CSR.select :flush @17:36:53.83 CS.seek 5ml @17:37:07.43 delay 5 seconds @17:37:12.45 CSR.select :air @17:37:12.57 CS.seek 10ml @17:37:26.15 CSV.dial CBV @17:37:27.28 CS.seek 0ml @17:37:53.72 Garaging FlushPuck in Collection Clamp @17:37:53.79 Elbow.reconfigure :tight @17:37:53.83 Elbow.seek CC @17:37:54.13 Elbow.reconfigure :loose @17:37:54.23 Forearm.seek CC @17:37:55.18 Hand.close @17:37:55.79 CC.seek :open @17:38:00.46 Forearm.seek :retract @17:38:01.86 Elbow.reconfigure :tight @17:38:01.92 Elbow.seek :garage @17:38:04.83 Elbow.reconfigure :loose @17:38:04.93 Forearm.reconfigure :tight @17:38:04.98 Forearm.seek :garage @17:38:05.97 Hand.open @17:38:06.60 Forearm.reconfigure :loose @17:38:06.65 Forearm.seek :retract @17:38:07.63 Completed [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] begun 15:13:28 @17:38:07.69 Asleep after completing DA_WCR_112823 assays @17:38:07.71 GoodNews.email "{1 => 33,\n 2 => 33,\n 3 => 14,\n 4 => 19,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@17:32:39, 29% flow, 17.5C, 28% humidity, 15.2psia, 13.725V, 0.345A, 101.877Ah, 4.73W\nSampled 400.0ml",Subject: "Asleep after completing DA_WCR_112823 assays" @17:38:11.49 upsync + ESPwhich DA_WCR_112823b.rb + fn=DA_WCR_112823b.rb + IFS=: + set /home/esp/esp2/mission . + fullPath=/home/esp/esp2/mission/DA_WCR_112823b.rb + [ -f /home/esp/esp2/mission/DA_WCR_112823b.rb ] + realpath /home/esp/esp2/mission/DA_WCR_112823b.rb + return 0 + scriptPath=/home/esp/esp2local/mission/DA_WCR_112823b.rb + exec ruby /home/esp/esp2/lib/esp.rb /home/esp/esp2local/mission/DA_WCR_112823b.rb @21:01:42.92UTC28-Nov-23
ESP2 v3.42 I2C Gateway v3.20 for core bus @21:01:45.80 Configuration for short puck jake 1/11/21 by brent@mbari.org /home/esp/esp2/type/shallow/jake/configure.rb @21:01:49.71 Polling Can every 10:00 @21:01:49.80 Cleanly exited at 20:58:03UTC28-Nov-23 @21:01:49.84 Loading /home/esp/esp2local/mission/DA_WCR_112823b.rb mission @21:01:49.90 GoodNews.email "Starting DA_WCR_112823b w/pucks in tube 4\n{1 => 33,\n 2 => 33,\n 3 => 14,\n 4 => 19,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@21:01:49, 30% flow, 17.2C, 29% humidity, 15.2psia, 13.754V, 0.266A, 102.737Ah, 3.66W",Subject: "DA_WCR_112823b begun" @21:01:49.93
Preparing to sleep until + 00:00:05 @21:01:49.98 Asleep til + 00:00:05 @21:01:50.00 Continuing at 21:01 @21:01:50.28 Gate.power :core,:OFF @21:01:50.30 Gate.power :raw,:OFF @21:01:50.31 Shutdown @21:01:52.78 GoodNews.email "Then, in tube 4: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_112823b.rb:14\n{1 => 33,\n 2 => 33,\n 3 => 14,\n 4 => 19,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@21:01:50, 30% flow, 17.2C, 29% humidity, 15.2psia, 13.754V, 0.266A, 102.737Ah, 3.66W",Subject: "Asleep until 21:01" @21:01:54.22 upsync @21:01:54.88
Powering core bus @21:01:56.85 upsync @21:02:00.36
Gate.power :raw,:ON @21:02:01.44 CS.configure :default @21:02:01.49 PS.configure :default @21:02:01.54 SS.configure :default @21:02:01.59 CC.configure :default @21:02:01.64 PC.configure :default @21:02:01.70 Forearm.configure :loose @21:02:01.75 Elbow.configure :loose @21:02:01.82 SC.configure :loose @21:02:01.87 SE.configure :default @21:02:01.91 CH.configure :default @21:02:01.95 PH.configure :default @21:02:01.99 All dwarf controllers running firmware version 3.85 @21:02:02.10 Valve.reset @21:02:02.33 Intake.select :close @21:02:02.39 Exhaust.select :close @21:02:02.43 CTV.dial :air @21:02:02.51 PSV.dial :air @21:02:02.57 CSV.dial :waste1 @21:02:02.69
Forearm.home.seek :retract @21:02:03.08 SSV.dial :spit,avoiding: :exhaust @21:02:03.43 Hand.close @21:02:03.47
Elbow.reconfigure :loose @21:02:03.56 Elbow.jog 1500 @21:02:03.65 PS.home.seek 0ml @21:02:03.95 CS.reconfigure :fast @21:02:03.98 SS.home.seek 0ml @21:02:04.09 CS.home.seek 0ml @21:02:04.17
Elbow.reconfigure :loose @21:02:04.21 Elbow.home.seek :clear @21:02:07.50 SE.home.seek :down @21:02:08.11 SC.reconfigure :tight @21:02:08.20 SC.home.seek :initial @21:02:12.00 PS.stop @21:02:23.94
SC.configure :limp @21:02:23.99 Forearm.seek :retract @21:02:24.06 Elbow.seek :hammer @21:02:25.11 Forearm.reconfigure :tight @21:02:25.16 Forearm.seek retracted @21:02:25.44 CC.home.seek :open @21:02:25.46 PC.home.seek :open @21:02:29.25
Phase [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] @21:02:29.27 Notice.email "At 21:02:29 began in tube 4: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_112823b.rb:14\n{1 => 33,\n 2 => 33,\n 3 => 14,\n 4 => 19,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@21:02:29, 30% flow, 17.2C, 29% humidity, 15.2psia, 13.676V, 0.290A, 102.740Ah, 3.97W",Subject: "Started DA_WCR_112823b phase initialPurge; da [1000, 1000] #da and wsr are run simultanelously" @21:02:29.30
Starting initialPurge @21:02:29.31 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @21:02:29.39 Hand.open @21:02:30.08 Elbow.reconfigure :tight @21:02:30.13 Elbow.seek FlushPuck::Garage @21:02:32.11 Elbow.reconfigure :loose @21:02:32.31 Forearm.reconfigure :loose @21:02:32.37 Forearm.seek :garage @21:02:33.32 Hand.close @21:02:33.39 upsync @21:02:34.07
Forearm.seek :retract @21:02:35.03 Elbow.reconfigure :tight @21:02:35.07 Elbow.seek CC @21:02:37.94 Elbow.reconfigure :loose @21:02:38.04 Forearm.reconfigure :tight @21:02:38.09 Forearm.seek CC @21:02:39.33 CC.seek :closed @21:02:43.87 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.42 @21:02:43.94 Hand.open @21:02:44.63 Forearm.reconfigure :loose @21:02:44.68 Forearm.seek :retract @21:02:45.73 Flushing the Collection Valve::Manifold @21:02:45.85 CSV.dial CSR @21:02:47.13 Pulling 5ml of CSR.flush @21:02:47.14 CSR.select :flush @21:02:47.25 CS.reconfigure :default @21:02:47.30 CS.seek 5ml @21:03:01.30 delay 5 seconds @21:03:06.32 CSR.select :air @21:03:06.49 CS.reconfigure :fast @21:03:06.54 CS.seek 7.5ml @21:03:13.39 CSV.dial :waste @21:03:14.69 CS.reconfigure :default @21:03:14.74 CS.seek 0ml @21:03:34.77 Purging external Intake @21:03:34.89 CSV.dial :flush @21:03:35.34 CS.reconfigure :fast @21:03:35.38 CS.seek 10ml @21:03:59.98 delay 5 seconds @21:04:05.01 CSV.dial CBV @21:04:05.05
CBV.dial CSV @21:04:05.77 CTV.dial :intake @21:04:06.55 Intake.select :open @21:04:06.66 CS.seek 0ml @21:04:30.23 Intake.select :close @21:04:30.34 CTV.dial :air @21:04:31.11 Flushing upper manifold @21:04:31.23 CSV.dial :flush @21:04:31.98 CS.seek 5ml @21:04:44.67 delay 5 seconds @21:04:49.69 CSV.dial :air @21:04:50.22 CS.seek 7ml @21:04:55.74 CSV.dial CBV @21:04:55.77
CBV.dial CSV @21:04:56.80 CTV.dial :air @21:04:56.88 CS.seek 0ml @21:05:13.58 delay 7 seconds @21:05:20.61 CS.seek 3ml @21:05:28.64 CSV.dial :waste @21:05:29.10 CS.seek 0ml @21:05:36.91 CSV.dial :flush @21:05:37.35 CS.seek 3ml @21:05:45.39 delay 5 seconds @21:05:50.41 CSV.dial :air @21:05:50.94 CS.seek 5ml @21:05:56.61 CSV.dial :air @21:05:56.79 CS.seek 0ml @21:06:08.89 delay 7 seconds @21:06:15.92 CS.seek 3ml @21:06:23.98 CSV.dial :waste @21:06:24.72 CS.seek 0ml @21:06:32.42 Garaging FlushPuck in Collection Clamp @21:06:32.49 Elbow.reconfigure :tight @21:06:32.54 Elbow.seek CC @21:06:32.89 Elbow.reconfigure :loose @21:06:32.99 Forearm.seek CC @21:06:34.03 Hand.close @21:06:34.69 CC.seek :open @21:06:39.75 Forearm.seek :retract @21:06:40.83 Elbow.reconfigure :tight @21:06:40.88 Elbow.seek :garage @21:06:43.70 Elbow.reconfigure :loose @21:06:43.80 Forearm.reconfigure :tight @21:06:43.85 Forearm.seek :garage @21:06:44.88 Hand.open @21:06:45.50 Forearm.reconfigure :loose @21:06:45.55 Forearm.seek :retract @21:06:46.49 da sampling at most 1000ml, wcr at most 1000ml @21:06:46.53 Loading sh1 puck from tube 4 into Collection Clamp @21:06:46.55 DAfans.email "Started da in tube 4 at 21:06:46UTC28-Nov-23\n{1 => 33,\n 2 => 33,\n 3 => 14,\n 4 => 19,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@21:02:29, 30% flow, 17.2C, 29% humidity, 15.2psia, 13.676V, 0.290A, 102.740Ah, 3.97W",Subject: "da sampling at most 1000ml, wcr at most 1000ml" @21:06:47.17
Elbow.reconfigure :tight @21:06:47.29 Elbow.seek 4 @21:06:49.39 Forearm.reconfigure :tight @21:06:49.50 Forearm.seek 4 + 450 counts @21:06:50.57 SE.reconfigure :pick @21:06:50.63 SE.seek :beamBreak @21:06:50.73 upsync @21:06:52.55
SE.configure :default @21:06:52.72 Elbow.seek 4 @21:06:52.91 Forearm.seek 4 + 300 counts @21:06:53.17 Forearm.reconfigure :loose @21:06:53.22 Hand.close @21:06:53.83 SE.seek :down @21:06:54.05
Forearm.seek :retract @21:06:55.01 Elbow.seek CC @21:06:55.52 SE.stop @21:06:57.03
Elbow.reconfigure :loose @21:06:57.13 Forearm.reconfigure :tight @21:06:57.18 Forearm.seek CC @21:06:58.39 CC.seek :closed @21:07:02.93 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.28 @21:07:02.94 Hand.open @21:07:03.63 Forearm.reconfigure :loose @21:07:03.67 Forearm.seek :retract @21:07:04.65 collectSample 1000ml w/bubble point of 24psi @21:07:04.66 Priming 7ml @21:07:04.67 Intake.select :close @21:07:04.68 Exhaust.select :close @21:07:04.69 SS.hold @21:07:04.90 Calibrating pressure and temperature sensors @21:07:04.93 CTV.dial :air @21:07:05.00 SSV.dial :spit,avoiding: :exhaust @21:07:05.08 SS.reconfigure :fast @21:07:05.13 SS.seek 5ml @21:07:07.93 SSV.dial CBV @21:07:07.96
CBV.dial SSV @21:07:09.31 delay 3.8 seconds @21:07:13.24 Sensor limits: Intake max 103.3psia; Outlet max 96.3psia @21:07:13.25 SSV.dial :spit @21:07:14.56 SS.seek 0ml @21:07:17.34 SSV.dial CBV @21:07:18.66 CTV.dial :intake @21:07:19.44 SSV.dial CBV @21:07:19.46
CBV.dial SSV @21:07:19.55 Allowing 40s for 7.0ml of remaining 7.0ml @21:07:19.56 Intake.select :open @21:07:21.72 Intake @14.3psia, vacuum from 8.8 to 11.8psi @21:07:21.74 SS.reconfigure :inhaling 2.20ml/s @21:07:21.79 SS.seek 7ml @21:07:28.23 Stroke completed -- waiting for pressure to equalize ... @21:07:28.24 SS.reconfigure :exhale @21:07:28.28 SS.hold @21:07:38.22 Intake.select :close @21:07:38.38 SSV.dial :exhaust @21:07:39.15 Exhaust.select :open @21:07:39.27 SS.seek 0ml @21:07:43.37 Exhaust.select :close @21:07:43.48 Sampling 1000.0ml @21:07:43.49 Intake.select :close @21:07:43.50 Exhaust.select :close @21:07:43.52 SS.reconfigure :default @21:07:43.56 SS.hold @21:07:43.77 CTV.dial :intake @21:07:43.94 SSV.dial CBV @21:07:43.96
CBV.dial SSV @21:07:44.73 Allowing 130s for 25.0ml of remaining 1000.0ml @21:07:44.74 Intake.select :open @21:07:46.91 Intake @15.1psia, vacuum from 9.6 to 12.6psi @21:07:46.92 SS.reconfigure :inhaling 2.20ml/s @21:07:46.97 SS.seek 25ml @21:07:54.96 Vacuum too high -- waiting up to 40 seconds @21:07:54.97 SS.reconfigure :exhale @21:07:55.02 SS.hold @21:08:01.43 Intake @17.2psia, vacuum from 10.0 to 13.0psi @21:08:01.45 SS.reconfigure :inhaling 1.76ml/s @21:08:01.50 SS.seek 25ml @21:08:04.55 Vacuum too high -- waiting up to 40 seconds @21:08:04.56 SS.reconfigure :exhale @21:08:04.62 SS.hold @21:08:07.85 Intake @17.5psia, vacuum from 10.0 to 13.0psi @21:08:07.86 SS.reconfigure :inhaling 1.41ml/s @21:08:07.91 SS.seek 25ml @21:08:10.93 Vacuum too high -- waiting up to 40 seconds @21:08:10.94 SS.reconfigure :exhale @21:08:10.99 SS.hold @21:08:13.61 SS.reconfigure :inhaling 1.13ml/s @21:08:13.66 SS.seek 25ml @21:08:18.11 Vacuum too high -- waiting up to 40 seconds @21:08:18.12 SS.reconfigure :exhale @21:08:18.17 SS.hold @21:08:20.27 Intake @16.9psia, vacuum from 10.0 to 13.0psi @21:08:20.29 SS.reconfigure :inhaling 0.91ml/s @21:08:20.34 SS.seek 25ml @21:08:23.59 Stroke completed -- waiting for pressure to equalize ... @21:08:23.60 SS.reconfigure :exhale @21:08:23.65 SS.hold @21:08:48.50 Intake.select :close @21:08:48.66 SSV.dial :exhaust @21:08:49.41 Exhaust.select :open @21:08:49.53 SS.seek 0ml @21:08:58.24 Exhaust.select :close @21:08:58.35 SSV.dial CBV @21:08:58.37
CBV.dial SSV @21:08:59.15 Allowing 130s for 25.0ml of remaining 975.0ml @21:08:59.16 Intake.select :open @21:09:01.33 Intake @15.6psia, vacuum from 10.0 to 13.0psi @21:09:01.35 SS.reconfigure :inhaling 0.91ml/s @21:09:01.40 SS.seek 25ml @21:09:31.83 Stroke completed -- waiting for pressure to equalize ... @21:09:31.84 SS.reconfigure :exhale @21:09:31.89 SS.hold @21:10:02.74 Intake.select :close @21:10:02.89 SSV.dial :exhaust @21:10:03.67 Exhaust.select :open @21:10:03.78 SS.seek 0ml @21:10:12.82 Exhaust.select :close @21:10:12.93 SSV.dial CBV @21:10:12.95
CBV.dial SSV @21:10:13.73 Allowing 130s for 25.0ml of remaining 950.0ml @21:10:13.74 Intake.select :open @21:10:15.90 Intake @15.7psia, vacuum from 10.0 to 13.0psi @21:10:15.91 SS.reconfigure :inhaling 0.91ml/s @21:10:15.96 SS.seek 25ml @21:10:45.53 Stroke completed -- waiting for pressure to equalize ... @21:10:45.54 SS.reconfigure :exhale @21:10:45.59 SS.hold @21:11:12.61 Intake.select :close @21:11:12.76 SSV.dial :exhaust @21:11:13.53 Exhaust.select :open @21:11:13.64 SS.seek 0ml @21:11:22.46 Exhaust.select :close @21:11:22.56 SSV.dial CBV @21:11:22.58
CBV.dial SSV @21:11:23.35 Allowing 130s for 25.0ml of remaining 925.0ml @21:11:23.36 Intake.select :open @21:11:25.53 Intake @15.9psia, vacuum from 10.0 to 13.0psi @21:11:25.54 SS.reconfigure :inhaling 0.91ml/s @21:11:25.59 SS.seek 25ml @21:11:55.64 Stroke completed -- waiting for pressure to equalize ... @21:11:55.65 SS.reconfigure :exhale @21:11:55.70 SS.hold @21:12:24.98 Intake.select :close @21:12:25.14 SSV.dial :exhaust @21:12:25.91 Exhaust.select :open @21:12:26.02 SS.seek 0ml @21:12:34.83 Exhaust.select :close @21:12:34.94 SSV.dial CBV @21:12:34.96
CBV.dial SSV @21:12:35.74 Allowing 130s for 25.0ml of remaining 900.0ml @21:12:35.76 Intake.select :open @21:12:37.93 Intake @15.8psia, vacuum from 10.0 to 13.0psi @21:12:37.94 SS.reconfigure :inhaling 0.91ml/s @21:12:37.99 SS.seek 25ml @21:13:05.23 Vacuum too high -- waiting up to 40 seconds @21:13:05.24 SS.reconfigure :exhale @21:13:05.29 SS.hold @21:13:13.31 Intake @17.3psia, vacuum from 10.0 to 13.0psi @21:13:13.32 SS.reconfigure :inhaling 0.73ml/s @21:13:13.37 SS.seek 25ml @21:13:16.78 Stroke completed -- waiting for pressure to equalize ... @21:13:16.79 SS.reconfigure :exhale @21:13:16.84 SS.hold @21:13:33.50 Intake.select :close @21:13:33.73 SSV.dial :exhaust @21:13:34.49 Exhaust.select :open @21:13:34.60 SS.seek 0ml @21:13:43.53 Exhaust.select :close @21:13:43.64 SSV.dial CBV @21:13:43.67
CBV.dial SSV @21:13:44.44 Allowing 130s for 25.0ml of remaining 875.0ml @21:13:44.45 Intake.select :open @21:13:46.62 Intake @15.8psia, vacuum from 10.0 to 13.0psi @21:13:46.64 SS.reconfigure :inhaling 0.73ml/s @21:13:46.69 SS.seek 25ml @21:14:22.67 Stroke completed -- waiting for pressure to equalize ... @21:14:22.68 SS.reconfigure :exhale @21:14:22.73 SS.hold @21:14:45.16 Intake.select :close @21:14:45.32 SSV.dial :exhaust @21:14:46.09 Exhaust.select :open @21:14:46.21 SS.seek 0ml @21:14:55.08 Exhaust.select :close @21:14:55.61 SSV.dial CBV @21:14:55.65
CBV.dial SSV @21:14:56.41 Allowing 130s for 25.0ml of remaining 850.0ml @21:14:56.42 Intake.select :open @21:14:58.59 Intake @15.8psia, vacuum from 10.0 to 13.0psi @21:14:58.61 SS.reconfigure :inhaling 0.73ml/s @21:14:58.65 SS.seek 25ml @21:15:34.47 Stroke completed -- waiting for pressure to equalize ... @21:15:34.48 SS.reconfigure :exhale @21:15:34.52 SS.hold @21:15:57.02 Intake.select :close @21:15:57.17 SSV.dial :exhaust @21:15:57.94 Exhaust.select :open @21:15:58.05 SS.seek 0ml @21:16:06.83 Exhaust.select :close @21:16:06.93 SSV.dial CBV @21:16:06.96
CBV.dial SSV @21:16:07.73 Allowing 130s for 25.0ml of remaining 825.0ml @21:16:07.75 Intake.select :open @21:16:09.91 Intake @15.9psia, vacuum from 10.0 to 13.0psi @21:16:09.93 SS.reconfigure :inhaling 0.73ml/s @21:16:09.98 SS.seek 25ml @21:16:46.82 Stroke completed -- waiting for pressure to equalize ... @21:16:46.83 SS.reconfigure :exhale @21:16:46.87 SS.hold @21:17:09.35 Intake.select :close @21:17:09.50 SSV.dial :exhaust @21:17:10.26 Exhaust.select :open @21:17:10.38 SS.seek 0ml @21:17:19.17 Exhaust.select :close @21:17:19.28 SSV.dial CBV @21:17:19.30
CBV.dial SSV @21:17:20.08 Allowing 130s for 25.0ml of remaining 800.0ml @21:17:20.09 Intake.select :open @21:17:22.25 Intake @15.8psia, vacuum from 10.0 to 13.0psi @21:17:22.27 SS.reconfigure :inhaling 0.73ml/s @21:17:22.32 SS.seek 25ml @21:17:56.88 Vacuum too high -- waiting up to 40 seconds @21:17:56.89 SS.reconfigure :exhale @21:17:56.95 SS.hold @21:18:05.94 Intake @17.1psia, vacuum from 10.0 to 13.0psi @21:18:05.96 SS.reconfigure :inhaling 0.59ml/s @21:18:06.01 SS.seek 25ml @21:18:08.37 Stroke completed -- waiting for pressure to equalize ... @21:18:08.38 SS.reconfigure :exhale @21:18:08.43 SS.hold @21:18:21.44 Intake.select :close @21:18:21.59 SSV.dial :exhaust @21:18:22.36 Exhaust.select :open @21:18:22.47 SS.seek 0ml @21:18:31.07 Exhaust.select :close @21:18:31.18 SSV.dial CBV @21:18:31.22
CBV.dial SSV @21:18:31.98 Allowing 130s for 25.0ml of remaining 775.0ml @21:18:32.00 Intake.select :open @21:18:34.16 Intake @15.9psia, vacuum from 10.0 to 13.0psi @21:18:34.17 SS.reconfigure :inhaling 0.59ml/s @21:18:34.22 SS.seek 25ml @21:19:19.04 Stroke completed -- waiting for pressure to equalize ... @21:19:19.05 SS.reconfigure :exhale @21:19:19.10 SS.hold @21:19:39.49 Intake.select :close @21:19:39.64 SSV.dial :exhaust @21:19:40.41 Exhaust.select :open @21:19:40.52 SS.seek 0ml @21:19:49.31 Exhaust.select :close @21:19:49.84 SSV.dial CBV @21:19:49.86
CBV.dial SSV @21:19:50.65 Allowing 130s for 25.0ml of remaining 750.0ml @21:19:50.66 Intake.select :open @21:19:52.83 Intake @15.7psia, vacuum from 10.0 to 13.0psi @21:19:52.85 SS.reconfigure :inhaling 0.59ml/s @21:19:52.90 SS.seek 25ml @21:20:37.48 Stroke completed -- waiting for pressure to equalize ... @21:20:37.49 SS.reconfigure :exhale @21:20:37.53 SS.hold @21:20:57.87 Intake.select :close @21:20:58.02 SSV.dial :exhaust @21:20:58.80 Exhaust.select :open @21:20:58.91 SS.seek 0ml @21:21:07.70 Exhaust.select :close @21:21:07.81 SSV.dial CBV @21:21:07.83
CBV.dial SSV @21:21:08.60 Allowing 130s for 25.0ml of remaining 725.0ml @21:21:08.62 Intake.select :open @21:21:10.78 Intake @15.8psia, vacuum from 10.0 to 13.0psi @21:21:10.79 SS.reconfigure :inhaling 0.59ml/s @21:21:10.84 SS.seek 25ml @21:21:55.85 Stroke completed -- waiting for pressure to equalize ... @21:21:55.86 SS.reconfigure :exhale @21:21:55.91 SS.hold @21:22:20.70 Intake.select :close @21:22:20.85 SSV.dial :exhaust @21:22:21.62 Exhaust.select :open @21:22:21.73 SS.seek 0ml @21:22:30.49 Exhaust.select :close @21:22:30.60 SSV.dial CBV @21:22:30.62
CBV.dial SSV @21:22:31.40 Allowing 130s for 25.0ml of remaining 700.0ml @21:22:31.41 Intake.select :open @21:22:33.58 Intake @15.7psia, vacuum from 10.0 to 13.0psi @21:22:33.59 SS.reconfigure :inhaling 0.59ml/s @21:22:33.64 SS.seek 25ml @21:23:18.58 Stroke completed -- waiting for pressure to equalize ... @21:23:18.59 SS.reconfigure :exhale @21:23:18.64 SS.hold @21:23:43.74 Intake.select :close @21:23:43.89 SSV.dial :exhaust @21:23:44.66 Exhaust.select :open @21:23:44.78 SS.seek 0ml @21:23:53.49 Exhaust.select :close @21:23:53.60 SSV.dial CBV @21:23:53.62
CBV.dial SSV @21:23:54.40 Allowing 130s for 25.0ml of remaining 675.0ml @21:23:54.41 Intake.select :open @21:23:56.58 Intake @15.7psia, vacuum from 10.0 to 13.0psi @21:23:56.59 SS.reconfigure :inhaling 0.59ml/s @21:23:56.64 SS.seek 25ml @21:24:41.83 Stroke completed -- waiting for pressure to equalize ... @21:24:41.84 SS.reconfigure :exhale @21:24:41.89 SS.hold @21:25:10.66 Intake.select :close @21:25:10.81 SSV.dial :exhaust @21:25:11.58 Exhaust.select :open @21:25:11.70 SS.seek 0ml @21:25:20.75 Exhaust.select :close @21:25:20.86 SSV.dial CBV @21:25:20.89
CBV.dial SSV @21:25:21.66 Allowing 130s for 25.0ml of remaining 650.0ml @21:25:21.67 Intake.select :open @21:25:23.84 Intake @15.7psia, vacuum from 10.0 to 13.0psi @21:25:23.86 SS.reconfigure :inhaling 0.59ml/s @21:25:23.91 SS.seek 25ml @21:26:08.42 Stroke completed -- waiting for pressure to equalize ... @21:26:08.43 SS.reconfigure :exhale @21:26:08.48 SS.hold @21:26:41.35 Intake.select :close @21:26:41.50 SSV.dial :exhaust @21:26:42.28 Exhaust.select :open @21:26:42.39 SS.seek 0ml @21:26:51.17 Exhaust.select :close @21:26:51.28 SSV.dial CBV @21:26:51.31
CBV.dial SSV @21:26:52.08 Allowing 130s for 25.0ml of remaining 625.0ml @21:26:52.09 Intake.select :open @21:26:54.26 Intake @15.7psia, vacuum from 10.0 to 13.0psi @21:26:54.28 SS.reconfigure :inhaling 0.59ml/s @21:26:54.33 SS.seek 25ml @21:27:39.48 Stroke completed -- waiting for pressure to equalize ... @21:27:39.49 SS.reconfigure :exhale @21:27:39.54 SS.hold @21:28:18.79 Intake.select :close @21:28:18.95 SSV.dial :exhaust @21:28:19.73 Exhaust.select :open @21:28:19.84 SS.seek 0ml @21:28:28.50 Exhaust.select :close @21:28:28.61 SSV.dial CBV @21:28:28.63
CBV.dial SSV @21:28:29.41 Allowing 130s for 25.0ml of remaining 600.0ml @21:28:29.42 Intake.select :open @21:28:31.66 Intake @15.7psia, vacuum from 10.0 to 13.0psi @21:28:31.67 SS.reconfigure :inhaling 0.59ml/s @21:28:31.72 SS.seek 25ml @21:29:07.30 Vacuum too high -- waiting up to 40 seconds @21:29:07.32 SS.reconfigure :exhale @21:29:07.37 SS.hold @21:29:23.85 Intake @16.0psia, vacuum from 10.0 to 13.0psi @21:29:23.87 SS.reconfigure :inhaling 0.47ml/s @21:29:23.92 SS.seek 25ml @21:29:35.69 Stroke completed -- waiting for pressure to equalize ... @21:29:35.70 SS.reconfigure :exhale @21:29:35.74 SS.hold @21:30:02.28 Intake.select :close @21:30:02.44 SSV.dial :exhaust @21:30:03.21 Exhaust.select :open @21:30:03.33 SS.seek 0ml @21:30:12.14 Exhaust.select :close @21:30:12.25 SSV.dial CBV @21:30:12.27
CBV.dial SSV @21:30:13.05 Allowing 130s for 25.0ml of remaining 575.0ml @21:30:13.06 Intake.select :open @21:30:15.23 Intake @15.7psia, vacuum from 10.0 to 13.0psi @21:30:15.24 SS.reconfigure :inhaling 0.47ml/s @21:30:15.29 SS.seek 25ml @21:31:11.04 Stroke completed -- waiting for pressure to equalize ... @21:31:11.05 SS.reconfigure :exhale @21:31:11.10 SS.hold @21:31:49.92 Intake.select :close @21:31:50.08 SSV.dial :exhaust @21:31:50.87 Exhaust.select :open @21:31:50.98 SS.seek 0ml @21:31:59.81 Exhaust.select :close @21:31:59.92 SSV.dial CBV @21:31:59.94
CBV.dial SSV @21:32:00.73 Allowing 130s for 25.0ml of remaining 550.0ml @21:32:00.74 Intake.select :open @21:32:02.91 Intake @15.7psia, vacuum from 10.0 to 13.0psi @21:32:02.92 SS.reconfigure :inhaling 0.47ml/s @21:32:02.97 SS.seek 25ml @21:32:58.98 Stroke completed -- waiting for pressure to equalize ... @21:32:58.99 SS.reconfigure :exhale @21:32:59.03 SS.hold @21:33:39.22 SS.reconfigure :default @21:33:39.27 SS.hold @21:33:39.34 Intake.select :close @21:33:39.50 ShallowSampler::Clogged in MAIN -- Intake @16psia & Outlet @14psia won't equalize after 475.0ml @21:33:39.51 SSV.dial :exhaust @21:33:40.70 Exhaust.select :open @21:33:40.82 SS.reconfigure :exhale @21:33:40.86 SS.seek 0ml @21:33:49.63 Exhaust.select :close @21:33:49.75 Sampled 475.0ml @21:33:49.76 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @21:33:49.77 Intake.select :close @21:33:49.84 CSV.dial :waste @21:33:49.97 SSV.dial CBV,avoiding: :exhaust @21:33:49.99
CBV.dial SSV @21:33:50.08 CS.seek 0ml @21:33:50.36 CSV.dial :air @21:33:51.21 CS.seek 10ml @21:33:51.90
CTV.dial :air @21:33:52.68 delay 15 seconds @21:34:07.70 SSV.dial :spit,avoiding: :exhaust @21:34:09.03 CTV.dial CSV,avoiding: :intake @21:34:09.07
SS.seek 0ml @21:34:09.19 SSV.dial CBV,avoiding: :exhaust @21:34:15.90 SSV.dial between CBV and plug @21:34:16.39 CBV.dial between SSV and waste2 @21:34:16.80 CSV.dial between air and CTV @21:34:17.14 CS.seek 8.481ml @21:34:21.53 CTV.dial CSV,avoiding: :air @21:34:21.55
CSV.dial CTV @21:34:21.92 CS.reconfigure :pressurize @21:34:21.96 CS.seek 0ml @21:34:36.25 BubblePoint: Intake @24.3psia, Outlet @0.8psia @21:34:36.26 CBV.dial SSV @21:34:36.60 SS.reconfigure :default @21:34:36.65 SS.seek 10ml @21:34:42.26 delay 15 seconds @21:34:57.39 Evacuated: Intake @23.9psia, Outlet @23.8psia @21:34:57.40 SSV.dial :spit,avoiding: :exhaust @21:34:58.44 SS.reconfigure :exhale @21:34:58.48 SS.seek 0ml @21:35:03.47 SS.reconfigure :fast @21:35:03.52 SS.seek 5ml @21:35:06.31 SSV.dial CBV,avoiding: :exhaust @21:35:06.33
CBV.dial SSV @21:35:07.70 CTV.dial :air,avoiding: :intake @21:35:09.07 CSV.dial CTV @21:35:09.10
SS.seek 10ml @21:35:10.22 CSV.dial :air @21:35:10.68 CS.reconfigure :fast @21:35:11.14 CS.seek 0ml @21:35:11.62
SSV.dial :spit,avoiding: :exhaust @21:35:12.95 SS.reconfigure :exhale @21:35:13.00 SS.seek 0ml @21:35:18.15 SS.reconfigure :fast @21:35:18.19 SS.seek 5ml @21:35:19.05 CS.stop @21:35:21.06 SSV.dial CBV,avoiding: :exhaust @21:35:21.08
CBV.dial SSV @21:35:23.41 SSV.dial :spit,avoiding: :exhaust @21:35:24.73 SS.reconfigure :default @21:35:24.78 SS.seek 0ml @21:35:27.87 SS.stop @21:35:27.91 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @21:35:27.92 Intake.select :close @21:35:27.99 CSV.dial :waste @21:35:28.13 SSV.dial CBV,avoiding: :exhaust @21:35:28.14
CBV.dial SSV @21:35:28.78 CS.seek 0ml @21:35:28.86 CSV.dial :air @21:35:29.50
CTV.dial :air @21:35:29.60 CS.seek 10ml @21:35:29.72
delay 15 seconds @21:35:44.74 SSV.dial :spit,avoiding: :exhaust @21:35:46.08 CTV.dial CSV,avoiding: :intake @21:35:46.11
SS.reconfigure :exhale @21:35:46.21 SS.seek 0ml @21:35:46.62 SSV.dial CBV,avoiding: :exhaust @21:35:54.14 SSV.dial between CBV and plug @21:35:54.64 CBV.dial between SSV and waste2 @21:35:55.05 CSV.dial between air and CTV @21:35:55.35 CS.seek 7.738ml @21:36:01.37 CTV.dial CSV,avoiding: :air @21:36:01.39
CSV.dial CTV @21:36:01.74 CS.reconfigure :pressurize @21:36:01.79 CS.seek 0ml @21:36:18.18 BubblePoint: Intake @24.2psia, Outlet @2.8psia @21:36:18.19 CBV.dial SSV @21:36:18.54 SS.reconfigure :default @21:36:18.59 SS.seek 10ml @21:36:24.21 delay 15 seconds @21:36:39.34 Evacuated: Intake @23.9psia, Outlet @24.2psia @21:36:39.35 SSV.dial :spit,avoiding: :exhaust @21:36:40.39 SS.reconfigure :exhale @21:36:40.44 SS.seek 0ml @21:36:45.41 SS.reconfigure :fast @21:36:45.88 SS.seek 5ml @21:36:48.81 SSV.dial CBV,avoiding: :exhaust @21:36:48.83
CBV.dial SSV @21:36:50.24 CTV.dial :air,avoiding: :intake @21:36:51.58 CSV.dial CTV @21:36:51.64
SS.seek 10ml @21:36:52.80 CSV.dial :air @21:36:53.24 CS.reconfigure :fast @21:36:53.29 CS.seek 0ml @21:36:54.27
SSV.dial :spit,avoiding: :exhaust @21:36:55.63 SS.reconfigure :exhale @21:36:55.68 SS.seek 0ml @21:36:57.70 CS.stop @21:37:00.80
SS.reconfigure :fast @21:37:00.85 SS.seek 5ml @21:37:03.70 SSV.dial CBV,avoiding: :exhaust @21:37:03.72
CBV.dial SSV @21:37:06.09 SSV.dial :spit,avoiding: :exhaust @21:37:07.42 SS.reconfigure :default @21:37:07.47 SS.seek 0ml @21:37:10.83 SS.stop @21:37:10.87 Lysing: Adding 2.0ml AqMeOH @21:37:10.89 CBV.dial between CSV and waste1,avoiding: :DBV @21:37:12.39 CTV.dial CSV @21:37:13.70 CSV.dial CSR @21:37:14.50 Pulling 1ml of CSR.AqMeOH @21:37:14.51 CSR.select :AqMeOH @21:37:14.63 CS.reconfigure :default @21:37:14.67 CS.seek 1ml @21:37:18.08 delay 5 seconds @21:37:23.10 CSR.select :air @21:37:23.27 CS.seek 6.001ml @21:37:36.92 delay 2 seconds @21:37:38.94 CSV.dial :waste2 @21:37:39.40 CS.reconfigure :fast @21:37:39.44 CS.seek 0ml @21:37:53.89 CSV.dial CSR @21:37:54.47 Pulling 2ml of CSR.AqMeOH @21:37:54.48 CSR.select :AqMeOH @21:37:54.60 CS.reconfigure :default @21:37:54.64 CS.seek 2ml @21:38:00.64 delay 5 seconds @21:38:05.66 CSR.select :air @21:38:05.83 CS.seek 5.001ml @21:38:14.52 delay 2 seconds @21:38:16.53 CSV.dial CTV @21:38:17.01 CS.seek 1ml @21:38:28.19 Before lysing Collection Puck Heater at 13.7C/26.9psia @21:38:28.21 Heating Puck.from 4, :sh1 in Collection Clamp to 70C for 10:00 @21:38:28.23 CH.seek 70C,8:00 @21:40:39.29 CH.hold 10:00,70C @21:40:39.49 Begun lysing Collection Puck Heater at 68C/34.0psia @21:50:39.51 Ended lysing Collection Puck Heater at 70C/32.8psia @21:50:39.53 CS.seek 0ml @21:50:42.92 CTV.dial between spare and air,avoiding: :intake @21:50:43.94 CSV.dial :air @21:50:44.39 CS.seek 5ml @21:50:58.22 CSV.dial CTV @21:50:58.68 delay 2 seconds @21:51:00.70 CSV.dial :air @21:51:01.15 delay 2 seconds @21:51:03.16 CSV.dial CTV @21:51:03.62 delay 2 seconds @21:51:05.64 CSV.dial :air @21:51:06.08 delay 2 seconds @21:51:08.11 CS.seek 0ml @21:51:21.71 Puck @65.3psia after final lysis pressurization @21:51:21.73 Recovering Lysate into Collection syringe @21:51:21.74 CSV.dial CBV @21:51:22.76 CBV.dial CSV @21:51:23.15 CS.seek 5ml @21:51:36.75 delay 5 seconds @21:51:41.77 CTV.dial :air @21:51:42.30 CS.seek 7ml @21:51:48.19 delay 5 seconds @21:51:53.21 CSV.dial :air2 @21:51:53.67 CS.seek 10ml @21:52:02.22 CSV.dial between air and CTV,avoiding: CBV @21:52:03.38 Lysing Complete @21:52:03.45 Storing sh1 puck in Collection Clamp to tube 3 @21:52:03.63 SC.reconfigure :tight @21:52:03.67 SC.seek 3 @21:52:07.25 SC.configure :limp @21:52:07.47 Elbow.reconfigure :tight @21:52:07.52 Elbow.seek 3 @21:52:09.27 Forearm.reconfigure :tight @21:52:09.32 Forearm.seek 3 + 450 counts @21:52:10.79 SE.reconfigure :pick @21:52:10.85 SE.seek :beamBreak @21:52:13.11 SE.configure :default @21:52:13.27 Elbow.reconfigure :limp @21:52:13.32 Forearm.reconfigure :loose @21:52:13.37 Forearm.seek 3 @21:52:13.64 Elbow.hold @21:52:13.71 Elbow.reconfigure :tight @21:52:13.81 SE.seek 13.12 Pucks below Top Plate @21:52:14.39 Forearm.force -20 @21:52:14.43 Forearm.seek 3 + 303 counts @21:52:14.71 Forearm.reconfigure :tight @21:52:14.76 Forearm.seek 3 + 450 counts @21:52:14.96 SE.seek 14.12 Pucks below Top Plate @21:52:15.52 Forearm.reconfigure :loose @21:52:15.57 Forearm.seek :retract @21:52:16.50 Elbow.seek CC @21:52:18.19 Elbow.reconfigure :loose @21:52:18.29 Forearm.seek CC @21:52:19.28 Hand.close @21:52:19.91 CC.seek :open @21:52:24.67 Forearm.seek :retract @21:52:25.63 Elbow.reconfigure :tight @21:52:25.68 Elbow.seek 3 @21:52:27.45 Elbow.reconfigure :limp @21:52:27.50 Forearm.seek 3 @21:52:28.55 Elbow.hold @21:52:28.61 Elbow.reconfigure :tight @21:52:28.66 Hand.open @21:52:29.85 Forearm.force -20 @21:52:29.89 Forearm.seek 3 + 309 counts @21:52:30.18 Forearm.reconfigure :tight @21:52:30.23 Forearm.seek 3 + 450 counts @21:52:30.51 SE.seek :down @21:52:31.64
Forearm.reconfigure :loose @21:52:31.69 Forearm.seek :retract @21:52:32.24 SE.stop @21:52:32.66
Tranferring DA @21:52:32.67 Beginning samplePassC @21:52:32.68 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @21:52:32.87 Elbow.seek FlushPuck::Garage @21:52:34.70 Elbow.reconfigure :loose @21:52:34.81 Forearm.seek :garage @21:52:35.81 Hand.close @21:52:36.53 Forearm.seek :retract @21:52:37.47 Elbow.reconfigure :tight @21:52:37.52 Elbow.seek CC @21:52:40.48 Elbow.reconfigure :loose @21:52:40.58 Forearm.reconfigure :tight @21:52:40.63 Forearm.seek CC @21:52:41.88 CC.seek :closed @21:52:46.39 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.35 @21:52:46.90 Hand.open @21:52:47.59 Forearm.reconfigure :loose @21:52:47.64 Forearm.seek :retract @21:52:48.69 PSV.dial PSR @21:52:50.09 Pulling 1.5ml of PSR.flush @21:52:50.10 PSR.select :flush @21:52:50.22 PS.seek 1.5ml @21:53:06.45 delay 5 seconds @21:53:11.47 PSV.dial :waste1 @21:53:11.94 PS.seek 0ml @21:53:28.07 PSV.dial PSR @21:53:28.54 PSR.select :air @21:53:28.66 PS.seek 2.5ml @21:53:55.08 PRV.dial CBV,avoiding: :ab2 @21:53:55.10
CBV.dial PRV @21:53:56.87 PSV.dial :mixloop @21:53:57.90 CTV.dial :air,avoiding: :intake @21:53:58.08 PTV.dial :PRVmixing @21:53:59.41 PS.seek 0ml @21:54:25.76 PSV.dial :air @21:54:26.34 PS.seek 2.5ml @21:54:52.95 PSV.dial :mixloop @21:54:53.41 PS.seek 0ml @21:55:19.70 PSV.dial :air @21:55:20.17 PS.seek 2.5ml @21:55:46.57 PSV.dial :mixloop @21:55:47.05 PS.seek 0ml @21:56:13.35 Garaging FlushPuck in Collection Clamp @21:56:13.42 Elbow.reconfigure :tight @21:56:13.47 Elbow.seek CC @21:56:13.76 Elbow.reconfigure :loose @21:56:13.86 Forearm.seek CC @21:56:14.87 Hand.close @21:56:15.53 CC.seek :open @21:56:20.15 Forearm.seek :retract @21:56:21.16 Elbow.reconfigure :tight @21:56:21.58 Elbow.seek :garage @21:56:24.57 Elbow.reconfigure :loose @21:56:24.67 Forearm.reconfigure :tight @21:56:24.72 Forearm.seek :garage @21:56:25.74 Hand.open @21:56:26.47 Forearm.reconfigure :loose @21:56:26.51 Forearm.seek :retract @21:56:27.50 PSV.dial :mixloop @21:56:27.57 PTV.dial :PRVmixing @21:56:27.59 PRV.dial CBV @21:56:27.61
CBV.dial PRV @21:56:27.75 CTV.dial :air,avoiding: :intake @21:56:27.77 Loading lyfil puck from tube 4 into Collection Clamp @21:56:28.01 SC.reconfigure :tight @21:56:28.06 SC.seek 4 @21:56:31.63 SC.configure :limp @21:56:31.85 Elbow.reconfigure :tight @21:56:31.90 Elbow.seek 4 @21:56:33.87 Forearm.reconfigure :tight @21:56:33.92 Forearm.seek 4 + 450 counts @21:56:34.95 SE.reconfigure :pick @21:56:35.01 SE.seek :beamBreak @21:56:36.95 SE.configure :default @21:56:37.12 Elbow.seek 4 @21:56:37.29 Forearm.seek 4 + 300 counts @21:56:37.53 Forearm.reconfigure :loose @21:56:37.57 Hand.close @21:56:38.17 SE.seek :down @21:56:38.37
Forearm.seek :retract @21:56:39.38 Elbow.seek CC @21:56:39.77 SE.stop @21:56:40.99
Elbow.reconfigure :loose @21:56:41.10 Forearm.reconfigure :tight @21:56:41.14 Forearm.seek CC @21:56:42.46 CC.seek :closed @21:56:46.98 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.13 @21:56:46.99 Hand.open @21:56:47.72 Forearm.reconfigure :loose @21:56:47.77 Forearm.seek :retract @21:56:48.70 CTV.dial CSV,avoiding: :intake @21:56:50.05 CSV.dial CTV @21:56:50.38 PS.seek 2.5ml @21:57:16.79 CS.seek 4.501ml @21:57:31.62 delay 8 seconds @21:57:39.65 PSV.dial :air @21:57:40.12 delay 2 seconds @21:57:42.13 PSV.dial :waste2 @21:57:42.87 PS.seek 0ml @21:58:09.19 CBV.dial between PRV and waste1 @21:58:09.51 CSV.dial :air @21:58:09.99 CS.seek 10ml @21:58:24.84 CSV.dial CTV @21:58:25.30 CS.seek 0ml @21:58:51.93 CBV.dial :waste1 @21:58:52.25 delay 5 seconds @21:58:57.27 CTV.dial :air,avoiding: :intake @21:58:58.68 Storing lyfil puck in Collection Clamp to tube 3 @21:58:59.29 SC.reconfigure :tight @21:58:59.36 SC.seek 3 @21:59:02.93 SC.configure :limp @21:59:03.15 Elbow.reconfigure :tight @21:59:03.20 Elbow.seek 3 @21:59:04.97 Forearm.reconfigure :tight @21:59:05.02 Forearm.seek 3 + 450 counts @21:59:06.06 SE.reconfigure :pick @21:59:06.11 SE.seek :beamBreak @21:59:08.20 SE.configure :default @21:59:08.37 Elbow.reconfigure :limp @21:59:08.42 Forearm.reconfigure :loose @21:59:08.46 Forearm.seek 3 @21:59:08.74 Elbow.hold @21:59:08.81 Elbow.reconfigure :tight @21:59:08.91 SE.seek 14.13 Pucks below Top Plate @21:59:09.37 Forearm.force -20 @21:59:09.42 Forearm.seek 3 + 294 counts @21:59:09.71 Forearm.reconfigure :tight @21:59:09.76 Forearm.seek 3 + 450 counts @21:59:10.03 SE.seek 15.13 Pucks below Top Plate @21:59:10.58 Forearm.reconfigure :loose @21:59:10.63 Forearm.seek :retract @21:59:11.56 Elbow.seek CC @21:59:13.26 Elbow.reconfigure :loose @21:59:13.36 Forearm.seek CC @21:59:14.32 Hand.close @21:59:14.91 CC.seek :open @21:59:20.07 Forearm.seek :retract @21:59:21.06 Elbow.reconfigure :tight @21:59:21.11 Elbow.seek 3 @21:59:22.79 Elbow.reconfigure :limp @21:59:22.84 Forearm.seek 3 @21:59:23.89 Elbow.hold @21:59:23.95 Elbow.reconfigure :tight @21:59:24.00 Hand.open @21:59:24.79 Forearm.force -20 @21:59:24.83 Forearm.seek 3 + 320 counts @21:59:25.11 Forearm.reconfigure :tight @21:59:25.16 Forearm.seek 3 + 450 counts @21:59:25.42 SE.seek :down @21:59:26.55
Forearm.reconfigure :loose @21:59:26.60 Forearm.seek :retract @21:59:27.10 SE.stop @21:59:27.54
PSV.dial :air @21:59:28.29 PS.seek 2.5ml @21:59:54.82 delay 1 second @21:59:55.83 PSV.dial :mixloop @21:59:56.29 delay 1 second @21:59:57.30 PSV.dial :air @21:59:57.77 delay 1 second @21:59:58.79 PSV.dial :mixloop @21:59:59.26 delay 1 second @22:00:00.27 PSV.dial :air @22:00:00.74 delay 1 second @22:00:01.76 PS.seek 0ml @22:00:28.07 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @22:00:28.26 Elbow.seek FlushPuck::Garage @22:00:30.11 Elbow.reconfigure :loose @22:00:30.21 Forearm.seek :garage @22:00:31.18 Hand.close @22:00:31.90 Forearm.seek :retract @22:00:32.89 Elbow.reconfigure :tight @22:00:32.94 Elbow.seek PC @22:00:34.19 Elbow.reconfigure :loose @22:00:34.29 Forearm.reconfigure :tight @22:00:34.34 Forearm.seek PC @22:00:35.64 PC.seek :closed @22:00:40.26 Processing Clamp onSpring/offSpring for 16/26 samples, on/off torque 1.63 @22:00:40.33 Hand.open @22:00:40.96 Forearm.reconfigure :loose @22:00:41.01 Forearm.seek :retract @22:00:41.96 PRV.dial :air @22:00:42.41 PSV.dial :mixloop @22:00:42.94 PS.seek 0.3ml @22:00:46.88 PSV.dial PSR @22:00:47.92 Pulling 0.4ml of PSR.air @22:00:47.93 PSR.select :air @22:00:47.96 PS.seek 0.7ml @22:00:52.94 delay 3 seconds @22:00:55.96 PSR.select :air @22:00:55.98 PS.seek 2.5ml @22:01:15.28 delay 3 seconds @22:01:18.30 PSV.dial :mixloop @22:01:19.35 PTV.dial :mixing @22:01:20.10 PBV.dial :waste2 @22:01:21.07 PS.seek 0ml @22:01:47.44 Garaging FlushPuck in Processing Clamp @22:01:47.51 Elbow.reconfigure :tight @22:01:47.56 Elbow.seek PC @22:01:48.13 Elbow.reconfigure :loose @22:01:48.23 Forearm.seek PC @22:01:49.18 Hand.close @22:01:49.78 PC.seek :open @22:01:54.58 Forearm.seek :retract @22:01:55.54 Elbow.reconfigure :tight @22:01:55.59 Elbow.seek :garage @22:01:56.96 Elbow.reconfigure :loose @22:01:57.06 Forearm.reconfigure :tight @22:01:57.11 Forearm.seek :garage @22:01:58.16 Hand.open @22:01:58.83 Forearm.reconfigure :loose @22:01:58.88 Forearm.seek :retract @22:01:59.90 Loading da puck from tube 4 into Processing Clamp @22:02:00.14 SC.reconfigure :tight @22:02:00.19 SC.seek 4 @22:02:03.76 SC.configure :limp @22:02:03.98 Elbow.reconfigure :tight @22:02:04.03 Elbow.seek 4 @22:02:05.89 Forearm.reconfigure :tight @22:02:05.94 Forearm.seek 4 + 450 counts @22:02:07.02 SE.reconfigure :pick @22:02:07.08 SE.seek :beamBreak @22:02:09.12 SE.configure :default @22:02:09.27 Elbow.seek 4 @22:02:09.46 Forearm.seek 4 + 300 counts @22:02:09.66 Forearm.reconfigure :loose @22:02:09.71 Hand.close @22:02:10.29 SE.seek :down @22:02:10.94
Forearm.seek :retract @22:02:11.92 Elbow.seek PC @22:02:12.02 SE.stop @22:02:14.70
Elbow.reconfigure :loose @22:02:14.80 Forearm.reconfigure :tight @22:02:14.85 Forearm.seek PC @22:02:16.13 PC.seek :closed @22:02:20.76 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.66 @22:02:20.77 Hand.open @22:02:21.47 Forearm.reconfigure :loose @22:02:21.51 Forearm.seek :retract @22:02:22.47 Heating Puck.from 4, :da in Processing Clamp to 28C for 2:00:00 @22:02:22.49 PH.seek 28C,5:00 @22:03:03.12 PH.hold 2:00:00,28C @22:03:03.17 delay 1:30 @22:04:33.22 PSR.select :air @22:04:33.24 PSV.dial PSR @22:04:34.30 Pulling 0.5ml of PSR.wash PSR.select :wash @22:04:34.43 PS.seek 0.5ml @22:04:40.31 delay 5 seconds @22:04:45.33 PSR.select :air @22:04:45.45 PS.seek 2ml @22:05:01.65 PTV.dial :puck @22:05:02.40 PSV.dial PTV @22:05:03.17 PBV.dial :waste1 @22:05:04.56 PS.seek 1.5ml @22:05:10.50 delay 3 seconds @22:05:13.52 PBV.dial between PSV and waste1 @22:05:13.88 PS.seek 0ml @22:05:30.03 delay 5:00 @22:10:30.05 recoverPSRreagent08 @22:10:30.06 PTV.dial :puck @22:10:30.13 PBV.dial :waste1 @22:10:30.51 delay 30 seconds @22:11:00.53 PBV.dial between PSV and waste1 @22:11:00.90 PSR.select :air @22:11:00.91 PSV.dial PSR @22:11:01.68 PS.reconfigure :fast @22:11:01.73 PS.seek 2.5ml @22:11:25.55 PSV.dial PTV @22:11:26.32 delay 5 seconds @22:11:31.34 PSV.dial PSR @22:11:32.09 delay 5 seconds @22:11:37.10 PSV.dial PTV @22:11:37.98 delay 5 seconds @22:11:43.00 PSV.dial :air @22:11:43.76 PS.seek 0ml @22:12:07.09 PTV.dial :PRVmixing,via: PRV @22:12:08.41 PSV.dial :mixloop @22:12:08.88 PRV.dial :ab1 @22:12:09.64 PS.reconfigure :slow @22:12:09.68 PS.seek 0.15ml @22:12:13.37 delay 2 seconds @22:12:15.39 CBV.dial PRV @22:12:15.41
PRV.dial CBV @22:12:15.98 PS.seek 0.35ml @22:12:20.58 delay 2 seconds @22:12:22.60 CBV.dial between PRV and PSV @22:12:22.92 PRV.dial :air @22:12:23.39 PS.seek 0.9ml @22:12:35.14 PRV.dial :waste2 @22:12:35.89 PS.seek 0.5ml @22:12:44.58 addPMLReagent08 @22:12:44.59 PSV.dial :air @22:12:45.06 PS.reconfigure :default @22:12:45.11 PS.seek 2ml @22:13:01.30 PSV.dial :PML @22:13:01.77 PTV.dial :mixing @22:13:02.52 PBV.dial :waste1 @22:13:02.94 PS.seek 1.85ml @22:13:05.30 PBV.dial between PSV and waste1 @22:13:05.67 PS.seek 0ml @22:13:25.55 delay 2 seconds @22:13:27.57 Beginning samplePassC @22:13:27.58 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @22:13:27.77 Elbow.reconfigure :tight @22:13:27.82 Elbow.seek FlushPuck::Garage @22:13:29.13 Elbow.reconfigure :loose @22:13:29.23 Forearm.seek :garage @22:13:30.23 Hand.close @22:13:31.02 Forearm.seek :retract @22:13:32.03 Elbow.reconfigure :tight @22:13:32.08 Elbow.seek CC @22:13:34.93 Elbow.reconfigure :loose @22:13:35.03 Forearm.reconfigure :tight @22:13:35.08 Forearm.seek CC @22:13:36.89 CC.seek :closed @22:13:41.49 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.48 @22:13:41.56 Hand.open @22:13:42.21 Forearm.reconfigure :loose @22:13:42.25 Forearm.seek :retract @22:13:43.25 PSV.dial PSR @22:13:44.32 Pulling 1.5ml of PSR.flush PSR.select :flush @22:13:44.45 PS.seek 1.5ml @22:14:00.64 delay 5 seconds @22:14:05.66 PSV.dial :waste1 @22:14:06.13 PS.seek 0ml @22:14:22.30 PSV.dial PSR @22:14:22.77 PSR.select :air @22:14:22.89 PS.seek 2.5ml @22:14:49.31 PRV.dial CBV,avoiding: :ab2 @22:14:49.33
CBV.dial PRV @22:14:50.97 PSV.dial :mixloop @22:14:52.02 CTV.dial :air,avoiding: :intake @22:14:52.09 PTV.dial :PRVmixing @22:14:52.87 PS.seek 0ml @22:15:19.20 PSV.dial :air @22:15:19.68 PS.seek 2.5ml @22:15:45.98 PSV.dial :mixloop @22:15:46.48 PS.seek 0ml @22:16:12.86 PSV.dial :air @22:16:13.34 PS.seek 2.5ml @22:16:39.64 PSV.dial :mixloop @22:16:40.12 PS.seek 0ml @22:17:06.55 Garaging FlushPuck in Collection Clamp @22:17:06.62 Elbow.reconfigure :tight @22:17:06.67 Elbow.seek CC @22:17:06.99 Elbow.reconfigure :loose @22:17:07.09 Forearm.seek CC @22:17:08.57 Hand.close @22:17:09.14 CC.seek :open @22:17:13.79 Forearm.seek :retract @22:17:14.77 Elbow.reconfigure :tight @22:17:14.81 Elbow.seek :garage @22:17:17.73 Elbow.reconfigure :loose @22:17:17.83 Forearm.reconfigure :tight @22:17:17.88 Forearm.seek :garage @22:17:18.89 Hand.open @22:17:19.58 Forearm.reconfigure :loose @22:17:19.63 Forearm.seek :retract @22:17:20.58 delayUntil "22:21:27.56" @22:17:20.61 Whole Cell Archive sampling 1000ml @22:17:20.62 Loading wcr puck from tube 4 into Collection Clamp @22:17:21.10 Elbow.reconfigure :tight @22:17:21.15 Elbow.seek 4 @22:17:23.08 Forearm.reconfigure :tight @22:17:23.13 Forearm.seek 4 + 450 counts @22:17:24.19 SE.reconfigure :pick @22:17:24.26 SE.seek :beamBreak @22:17:26.33 SE.configure :default @22:17:26.49 Elbow.seek 4 @22:17:26.68 Forearm.seek 4 + 300 counts @22:17:26.90 Forearm.reconfigure :loose @22:17:26.95 Hand.close @22:17:27.53 SE.seek :down @22:17:28.20 Forearm.seek :retract @22:17:29.16 Elbow.seek CC @22:17:29.80 SE.stop @22:17:30.79 Elbow.reconfigure :loose @22:17:30.90 Forearm.reconfigure :tight @22:17:30.95 Forearm.seek CC @22:17:32.16 CC.seek :closed @22:17:36.68 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.42 @22:17:36.69 Hand.open @22:17:37.32 Forearm.reconfigure :loose @22:17:37.38 Forearm.seek :retract @22:17:38.34 collectSample 1000ml w/bubble point of 35psi @22:17:38.36 Priming 7ml @22:17:38.37 Intake.select :close @22:17:38.38 Exhaust.select :close @22:17:38.39 SS.hold @22:17:38.62 Calibrating pressure sensors @22:17:38.63 CTV.dial :air @22:17:38.72 SSV.dial :spit,avoiding: :exhaust @22:17:38.80 SS.reconfigure :fast @22:17:38.85 SS.seek 5ml @22:17:41.59 SSV.dial CBV @22:17:41.61 CBV.dial SSV @22:17:42.99 delay 3.8 seconds @22:17:46.93 Sensor limits: Intake max 104.5psia; Outlet max 96.0psia @22:17:46.95 SSV.dial :spit @22:17:48.28 SS.seek 0ml @22:17:51.44 SSV.dial CBV @22:17:53.23 CTV.dial :intake @22:17:54.00 SSV.dial CBV @22:17:54.02 CBV.dial SSV @22:17:54.19 Allowing 40s for 7.0ml of remaining 7.0ml @22:17:54.20 Intake.select :open @22:17:56.37 Intake @14.3psia, vacuum from 8.8 to 11.8psi @22:17:56.39 SS.reconfigure :inhaling 2.20ml/s @22:17:56.44 SS.seek 7ml @22:18:02.30 Stroke completed -- waiting for pressure to equalize ... @22:18:02.32 SS.reconfigure :exhale @22:18:02.36 SS.hold @22:18:24.91 Intake.select :close @22:18:25.07 SSV.dial :exhaust @22:18:25.85 Exhaust.select :open @22:18:25.97 SS.seek 0ml @22:18:30.04 Exhaust.select :close @22:18:30.15 Sampling 1000.0ml @22:18:30.17 Intake.select :close @22:18:30.18 Exhaust.select :close @22:18:30.19 SS.reconfigure :default @22:18:30.23 SS.hold @22:18:30.45 CTV.dial :intake @22:18:30.69 SSV.dial CBV @22:18:30.71 CBV.dial SSV @22:18:31.51 Allowing 130s for 25.0ml of remaining 1000.0ml @22:18:31.52 Intake.select :open @22:18:33.69 Intake @15.0psia, vacuum from 9.5 to 12.5psi @22:18:33.71 SS.reconfigure :inhaling 2.20ml/s @22:18:33.76 SS.seek 25ml @22:18:40.84 Vacuum too high -- waiting up to 40 seconds @22:18:40.86 SS.reconfigure :exhale @22:18:40.91 SS.hold @22:18:49.21 Intake @15.6psia, vacuum from 10.0 to 13.0psi @22:18:49.23 SS.reconfigure :inhaling 1.76ml/s @22:18:49.29 SS.seek 25ml @22:18:56.87 Vacuum too high -- waiting up to 40 seconds @22:18:56.89 SS.reconfigure :exhale @22:18:56.94 SS.hold @22:19:07.08 Intake @17.3psia, vacuum from 10.0 to 13.0psi @22:19:07.09 SS.reconfigure :inhaling 1.41ml/s @22:19:07.15 SS.seek 25ml @22:19:10.72 Vacuum too high -- waiting up to 40 seconds @22:19:10.73 SS.reconfigure :exhale @22:19:10.79 SS.hold @22:19:14.19 Intake @17.7psia, vacuum from 10.0 to 13.0psi @22:19:14.21 SS.reconfigure :inhaling 1.13ml/s @22:19:14.26 SS.seek 25ml @22:19:14.44 Stroke completed -- waiting for pressure to equalize ... @22:19:14.45 SS.reconfigure :exhale @22:19:14.50 SS.hold @22:19:41.10 Intake.select :close @22:19:41.26 SSV.dial :exhaust @22:19:42.02 Exhaust.select :open @22:19:42.14 SS.seek 0ml @22:19:51.00 Exhaust.select :close @22:19:51.11 SSV.dial CBV @22:19:51.16 CBV.dial SSV @22:19:51.90 Allowing 130s for 25.0ml of remaining 975.0ml @22:19:51.91 Intake.select :open @22:19:54.08 Intake @15.6psia, vacuum from 10.0 to 13.0psi @22:19:54.10 SS.reconfigure :inhaling 1.13ml/s @22:19:54.15 SS.seek 25ml @22:20:11.34 Vacuum too high -- waiting up to 40 seconds @22:20:11.36 SS.reconfigure :exhale @22:20:11.41 SS.hold @22:20:20.56 Intake @18.4psia, vacuum from 10.0 to 13.0psi @22:20:20.58 SS.reconfigure :inhaling 0.91ml/s @22:20:20.63 SS.seek 25ml @22:20:25.50 Vacuum too high -- waiting up to 40 seconds @22:20:25.51 SS.reconfigure :exhale @22:20:25.57 SS.hold @22:20:28.15 Intake @18.0psia, vacuum from 10.0 to 13.0psi @22:20:28.17 SS.reconfigure :inhaling 0.73ml/s @22:20:28.22 SS.seek 25ml @22:20:34.37 Vacuum too high -- waiting up to 40 seconds @22:20:34.39 SS.reconfigure :exhale @22:20:34.44 SS.hold @22:20:37.06 Intake @18.1psia, vacuum from 10.0 to 13.0psi @22:20:37.08 SS.reconfigure :inhaling 0.59ml/s @22:20:37.13 SS.seek 25ml @22:20:38.05 Stroke completed -- waiting for pressure to equalize ... @22:20:38.06 SS.reconfigure :exhale @22:20:38.11 SS.hold @22:21:05.17 Intake.select :close @22:21:05.33 SSV.dial :exhaust @22:21:06.10 Exhaust.select :open @22:21:06.22 SS.seek 0ml @22:21:15.00 Exhaust.select :close @22:21:15.11 SSV.dial CBV @22:21:15.15 CBV.dial SSV @22:21:15.91 Allowing 130s for 25.0ml of remaining 950.0ml @22:21:15.93 Intake.select :open @22:21:18.54 Intake @15.7psia, vacuum from 10.0 to 13.0psi @22:21:18.56 SS.reconfigure :inhaling 0.59ml/s @22:21:18.61 SS.seek 25ml @22:21:27.57 recoverPRVreagent08 @22:21:27.58 PTV.dial :mixing @22:21:28.34 PBV.dial :waste1 @22:21:28.72 delay 30 seconds @22:21:58.74 PBV.dial between PSV and waste1 @22:21:59.12 PSV.dial :air @22:21:59.72 PS.reconfigure :fast @22:21:59.77 PS.seek 2.5ml @22:22:03.82 Stroke completed -- waiting for pressure to equalize ... @22:22:03.83 SS.reconfigure :exhale @22:22:03.88 SS.hold @22:22:23.79 PSV.dial :mixloop @22:22:24.24 delay 2 seconds @22:22:26.26 PSV.dial :air @22:22:26.73 delay 2 seconds @22:22:28.75 PSV.dial :mixloop @22:22:29.21 delay 2 seconds @22:22:31.23 PSV.dial :air @22:22:31.72 PS.seek 0ml @22:22:32.39 Intake.select :close @22:22:32.55 SSV.dial :exhaust @22:22:33.31 Exhaust.select :open @22:22:33.43 SS.seek 0ml @22:22:42.14 Exhaust.select :close @22:22:42.25 SSV.dial CBV @22:22:42.29 CBV.dial SSV @22:22:43.07 Allowing 130s for 25.0ml of remaining 925.0ml @22:22:43.08 Intake.select :open @22:22:45.25 Intake @15.7psia, vacuum from 10.0 to 13.0psi @22:22:45.27 SS.reconfigure :inhaling 0.59ml/s @22:22:45.32 SS.seek 25ml @22:22:55.45 washCycleDA #1 of 2 @22:22:55.46 PSR.select :air @22:22:55.48 PSV.dial PSR @22:22:56.82 Pulling 0.5ml of PSR.wash @22:22:56.83 PSR.select :wash @22:22:56.95 PS.reconfigure :default @22:22:57.00 PS.seek 0.5ml @22:23:02.98 delay 5 seconds @22:23:08.00 PSR.select :air @22:23:08.12 PS.seek 2ml @22:23:24.26 PTV.dial :puck @22:23:25.01 PSV.dial PTV @22:23:25.76 PBV.dial :waste1 @22:23:26.20 PS.seek 1.5ml @22:23:30.47 Stroke completed -- waiting for pressure to equalize ... @22:23:30.48 SS.reconfigure :exhale @22:23:30.53 SS.hold @22:23:32.29 delay 3 seconds @22:23:35.32 PBV.dial between PSV and waste1 @22:23:35.70 PS.seek 0ml @22:23:51.82 delay 2:00 @22:23:55.30 Intake.select :close @22:23:55.46 SSV.dial :exhaust @22:23:56.22 Exhaust.select :open @22:23:56.34 SS.seek 0ml @22:24:05.14 Exhaust.select :close @22:24:05.26 SSV.dial CBV @22:24:05.30 CBV.dial SSV @22:24:06.06 Allowing 130s for 25.0ml of remaining 900.0ml @22:24:06.08 Intake.select :open @22:24:08.70 Intake @15.8psia, vacuum from 10.0 to 13.0psi @22:24:08.72 SS.reconfigure :inhaling 0.59ml/s @22:24:08.77 SS.seek 25ml @22:24:53.87 Stroke completed -- waiting for pressure to equalize ... @22:24:53.89 SS.reconfigure :exhale @22:24:53.93 SS.hold @22:25:18.46 Intake.select :close @22:25:19.17 SSV.dial :exhaust @22:25:19.94 Exhaust.select :open @22:25:20.06 SS.seek 0ml @22:25:29.01 Exhaust.select :close @22:25:29.12 SSV.dial CBV @22:25:29.15 CBV.dial SSV @22:25:29.92 Allowing 130s for 25.0ml of remaining 875.0ml @22:25:29.94 Intake.select :open @22:25:32.10 Intake @15.7psia, vacuum from 10.0 to 13.0psi @22:25:32.12 SS.reconfigure :inhaling 0.59ml/s @22:25:32.17 SS.seek 25ml @22:25:51.85 recoverPSRreagent08 @22:25:51.86 PTV.dial :puck @22:25:51.94 PBV.dial :waste1 @22:25:52.32 delay 30 seconds @22:26:16.71 Stroke completed -- waiting for pressure to equalize ... @22:26:16.73 SS.reconfigure :exhale @22:26:16.78 SS.hold @22:26:22.40 PBV.dial between PSV and waste1 @22:26:22.77 PSR.select :air @22:26:22.79 PSV.dial PSR @22:26:23.68 PS.reconfigure :fast @22:26:23.73 PS.seek 2.5ml @22:26:37.65 Intake.select :close @22:26:37.81 SSV.dial :exhaust @22:26:38.58 Exhaust.select :open @22:26:38.70 SS.seek 0ml @22:26:47.50 Exhaust.select :close @22:26:47.61 SSV.dial CBV @22:26:47.64 CBV.dial SSV @22:26:47.85 PSV.dial PTV @22:26:48.41 Allowing 130s for 25.0ml of remaining 850.0ml @22:26:48.42 Intake.select :open @22:26:48.62 delay 5 seconds @22:26:50.59 Intake @16.0psia, vacuum from 10.0 to 13.0psi @22:26:50.61 SS.reconfigure :inhaling 0.59ml/s @22:26:50.66 SS.seek 25ml @22:26:53.64 PSV.dial PSR @22:26:54.40 delay 5 seconds @22:26:59.42 PSV.dial PTV @22:27:00.18 delay 5 seconds @22:27:05.20 PSV.dial :air @22:27:05.96 PS.seek 0ml @22:27:29.60 washCycleDA #2 of 2 @22:27:29.61 PSR.select :air @22:27:29.63 PSV.dial PSR @22:27:30.97 Pulling 0.5ml of PSR.wash @22:27:30.98 PSR.select :wash @22:27:31.11 PS.reconfigure :default @22:27:31.15 PS.seek 0.5ml @22:27:35.56 Stroke completed -- waiting for pressure to equalize ... @22:27:35.57 SS.reconfigure :exhale @22:27:35.62 SS.hold @22:27:37.13 delay 5 seconds @22:27:42.14 PSR.select :air @22:27:42.31 PS.seek 2ml @22:27:58.28 Intake.select :close @22:27:58.44 SSV.dial :exhaust @22:27:58.51 PTV.dial :puck @22:27:58.58 PSV.dial PTV @22:27:59.20 Exhaust.select :open @22:27:59.34 PBV.dial :waste1 @22:27:59.36 SS.seek 0ml @22:27:59.83 PS.seek 1.5ml @22:28:05.85 delay 3 seconds @22:28:08.18 Exhaust.select :close @22:28:08.29 SSV.dial CBV @22:28:08.32 CBV.dial SSV @22:28:08.86 PBV.dial between PSV and waste1 @22:28:09.09 Allowing 130s for 25.0ml of remaining 825.0ml @22:28:09.11 Intake.select :open @22:28:09.23 PS.seek 0ml @22:28:11.80 Intake @15.7psia, vacuum from 10.0 to 13.0psi @22:28:11.82 SS.reconfigure :inhaling 0.59ml/s @22:28:11.87 SS.seek 25ml @22:28:25.79 delay 2:00 @22:28:56.60 Stroke completed -- waiting for pressure to equalize ... @22:28:56.62 SS.reconfigure :exhale @22:28:56.67 SS.hold @22:29:21.65 Intake.select :close @22:29:21.82 SSV.dial :exhaust @22:29:22.61 Exhaust.select :open @22:29:22.73 SS.seek 0ml @22:29:31.43 Exhaust.select :close @22:29:31.54 SSV.dial CBV @22:29:31.56 CBV.dial SSV @22:29:32.34 Allowing 130s for 25.0ml of remaining 800.0ml @22:29:32.36 Intake.select :open @22:29:34.52 Intake @15.6psia, vacuum from 10.0 to 13.0psi @22:29:34.54 SS.reconfigure :inhaling 0.59ml/s @22:29:34.59 SS.seek 25ml @22:30:19.59 Stroke completed -- waiting for pressure to equalize ... @22:30:19.60 SS.reconfigure :exhale @22:30:19.65 SS.hold @22:30:25.81 recoverPSRreagent08 @22:30:25.82 PTV.dial :puck @22:30:25.89 PBV.dial :waste1 @22:30:26.27 delay 30 seconds @22:30:44.18 Intake.select :close @22:30:44.34 SSV.dial :exhaust @22:30:45.10 Exhaust.select :open @22:30:45.22 SS.seek 0ml @22:30:53.90 Exhaust.select :close @22:30:54.02 SSV.dial CBV @22:30:54.04 CBV.dial SSV @22:30:54.84 Allowing 130s for 25.0ml of remaining 775.0ml @22:30:54.85 Intake.select :open @22:30:56.29 PBV.dial between PSV and waste1 @22:30:56.66 PSR.select :air @22:30:56.68 PSV.dial PSR @22:30:57.02 Intake @15.7psia, vacuum from 10.0 to 13.0psi @22:30:57.04 SS.reconfigure :inhaling 0.59ml/s @22:30:57.09 SS.seek 25ml @22:30:57.57 PS.reconfigure :fast @22:30:57.62 PS.seek 2.5ml @22:31:21.57 PSV.dial PTV @22:31:22.32 delay 5 seconds @22:31:26.05 Vacuum too high -- waiting up to 40 seconds @22:31:26.07 SS.reconfigure :exhale @22:31:26.12 SS.hold @22:31:27.33 PSV.dial PSR @22:31:28.09 delay 5 seconds @22:31:33.11 PSV.dial PTV @22:31:33.63 Intake @16.2psia, vacuum from 10.0 to 13.0psi @22:31:33.65 SS.reconfigure :inhaling 0.47ml/s @22:31:33.69 SS.seek 25ml @22:31:33.88 delay 5 seconds @22:31:38.90 PSV.dial :air @22:31:39.68 PS.seek 0ml @22:31:53.58 Stroke completed -- waiting for pressure to equalize ... @22:31:53.60 SS.reconfigure :exhale @22:31:53.65 SS.hold @22:32:03.22 getPMLReagent08 @22:32:03.23 PRV.dial :air @22:32:03.89 delay 5 seconds @22:32:08.92 PTV.dial :PRVmixing @22:32:10.25 delay 5 seconds @22:32:12.54 Intake.select :close @22:32:12.70 SSV.dial :exhaust @22:32:13.46 Exhaust.select :open @22:32:13.59 SS.seek 0ml @22:32:15.27 PRV.dial :ab2 @22:32:15.73 PTV.dial :PRVmixing @22:32:15.75 PSV.dial :mixloop @22:32:16.24 PS.reconfigure :default @22:32:16.29 PS.seek 0.3ml @22:32:20.22 delay 2 seconds @22:32:22.24 PRV.dial :air @22:32:22.37 Exhaust.select :close @22:32:22.48 SSV.dial CBV @22:32:22.50 CBV.dial SSV @22:32:22.78 PS.seek 0.55ml @22:32:23.29 Allowing 130s for 25.0ml of remaining 750.0ml @22:32:23.31 Intake.select :open @22:32:25.48 Intake @15.7psia, vacuum from 10.0 to 13.0psi @22:32:25.50 SS.reconfigure :inhaling 0.47ml/s @22:32:25.55 SS.seek 25ml @22:32:26.19 addPMLReagent08 @22:32:26.20 PSV.dial :air @22:32:26.68 PS.seek 2ml @22:32:42.28 PSV.dial :PML @22:32:42.76 PTV.dial :mixing @22:32:43.53 PBV.dial :waste1 @22:32:43.97 PS.seek 1.85ml @22:32:46.37 PBV.dial between PSV and waste1 @22:32:46.76 PS.seek 0ml @22:33:06.50 delay 2 seconds @22:33:08.52 delay 8:00 @22:33:21.08 Stroke completed -- waiting for pressure to equalize ... @22:33:21.10 SS.reconfigure :exhale @22:33:21.15 SS.hold @22:33:46.00 Intake.select :close @22:33:46.21 SSV.dial :exhaust @22:33:46.97 Exhaust.select :open @22:33:47.09 SS.seek 0ml @22:33:55.82 Exhaust.select :close @22:33:55.93 SSV.dial CBV @22:33:55.96 CBV.dial SSV @22:33:56.74 Allowing 130s for 25.0ml of remaining 725.0ml @22:33:56.76 Intake.select :open @22:33:59.32 Intake @15.6psia, vacuum from 10.0 to 13.0psi @22:33:59.41 SS.reconfigure :inhaling 0.47ml/s @22:33:59.46 SS.seek 25ml @22:34:55.08 Stroke completed -- waiting for pressure to equalize ... @22:34:55.09 SS.reconfigure :exhale @22:34:55.15 SS.hold @22:35:23.78 Intake.select :close @22:35:23.94 SSV.dial :exhaust @22:35:24.72 Exhaust.select :open @22:35:24.84 SS.seek 0ml @22:35:33.89 Exhaust.select :close @22:35:34.00 SSV.dial CBV @22:35:34.05 CBV.dial SSV @22:35:34.79 Allowing 130s for 25.0ml of remaining 700.0ml @22:35:34.80 Intake.select :open @22:35:36.98 Intake @15.6psia, vacuum from 10.0 to 13.0psi @22:35:37.00 SS.reconfigure :inhaling 0.47ml/s @22:35:37.04 SS.seek 25ml @22:36:32.41 Stroke completed -- waiting for pressure to equalize ... @22:36:32.42 SS.reconfigure :exhale @22:36:32.47 SS.hold @22:37:07.24 Intake.select :close @22:37:07.41 SSV.dial :exhaust @22:37:08.17 Exhaust.select :open @22:37:08.29 SS.seek 0ml @22:37:17.33 Exhaust.select :close @22:37:17.44 SSV.dial CBV @22:37:17.48 CBV.dial SSV @22:37:18.24 Allowing 130s for 25.0ml of remaining 675.0ml @22:37:18.25 Intake.select :open @22:37:20.43 Intake @15.7psia, vacuum from 10.0 to 13.0psi @22:37:20.45 SS.reconfigure :inhaling 0.47ml/s @22:37:20.49 SS.seek 25ml @22:38:11.82 Vacuum too high -- waiting up to 40 seconds @22:38:11.84 SS.reconfigure :exhale @22:38:11.89 SS.hold @22:38:32.41 Intake @16.4psia, vacuum from 10.0 to 13.0psi @22:38:32.43 SS.reconfigure :inhaling 0.38ml/s @22:38:32.48 SS.seek 25ml @22:38:37.37 Stroke completed -- waiting for pressure to equalize ... @22:38:37.39 SS.reconfigure :exhale @22:38:37.43 SS.hold @22:38:55.85 Intake.select :close @22:38:56.01 SSV.dial :exhaust @22:38:56.80 Exhaust.select :open @22:38:56.92 SS.seek 0ml @22:39:05.75 Exhaust.select :close @22:39:05.86 SSV.dial CBV @22:39:05.90 CBV.dial SSV @22:39:06.69 Allowing 130s for 25.0ml of remaining 650.0ml @22:39:06.70 Intake.select :open @22:39:08.87 Intake @15.6psia, vacuum from 10.0 to 13.0psi @22:39:08.89 SS.reconfigure :inhaling 0.38ml/s @22:39:08.94 SS.seek 25ml @22:40:17.99 Stroke completed -- waiting for pressure to equalize ... @22:40:18.01 SS.reconfigure :exhale @22:40:18.05 SS.hold @22:40:55.42 Intake.select :close @22:40:55.58 SSV.dial :exhaust @22:40:56.36 Exhaust.select :open @22:40:56.48 SS.seek 0ml @22:41:05.28 Exhaust.select :close @22:41:05.40 SSV.dial CBV @22:41:05.42 CBV.dial SSV @22:41:06.27 Allowing 130s for 25.0ml of remaining 625.0ml @22:41:06.28 Intake.select :open @22:41:08.45 Intake @15.6psia, vacuum from 10.0 to 13.0psi @22:41:08.47 SS.reconfigure :inhaling 0.38ml/s @22:41:08.52 SS.seek 25ml @22:41:08.58 recoverPRVreagent08 @22:41:08.59 PTV.dial :mixing @22:41:08.66 PBV.dial :waste1 @22:41:09.04 delay 30 seconds @22:41:39.06 PBV.dial between PSV and waste1 @22:41:39.45 PSV.dial :air @22:41:39.93 PS.reconfigure :fast @22:41:39.98 PS.seek 2.5ml @22:42:04.08 PSV.dial :mixloop @22:42:04.57 delay 2 seconds @22:42:06.59 PSV.dial :air @22:42:07.18 delay 2 seconds @22:42:09.20 PSV.dial :mixloop @22:42:09.65 delay 2 seconds @22:42:11.67 PSV.dial :air @22:42:12.14 PS.seek 0ml @22:42:17.20 Stroke completed -- waiting for pressure to equalize ... @22:42:17.21 SS.reconfigure :exhale @22:42:17.26 SS.hold @22:42:35.71 washCycleDA #1 of 3 @22:42:35.72 PSR.select :air @22:42:35.74 PSV.dial PSR @22:42:37.08 Pulling 0.5ml of PSR.wash @22:42:37.09 PSR.select :wash @22:42:37.21 PS.reconfigure :default @22:42:37.26 PS.seek 0.5ml @22:42:43.17 delay 5 seconds @22:42:48.19 PSR.select :air @22:42:48.31 PS.seek 2ml @22:42:57.45 SS.reconfigure :default @22:42:57.51 SS.hold @22:42:57.59 Intake.select :close @22:42:57.75 ShallowSampler::Clogged in WCR -- Intake @16psia & Outlet @13psia won't equalize after 400.0ml @22:42:57.76 SSV.dial :exhaust @22:42:58.54 Exhaust.select :open @22:42:58.66 SS.reconfigure :exhale @22:42:58.71 SS.seek 0ml @22:43:04.49 PTV.dial :puck @22:43:05.24 PSV.dial PTV @22:43:05.99 PBV.dial :waste1 @22:43:06.42 PS.seek 1.5ml @22:43:07.78 Exhaust.select :close @22:43:08.30 Sampled 400.0ml @22:43:08.32 Drying puck before fixing for 5:00 @22:43:08.33 CBV.dial :waste2b @22:43:09.10 delay 1:00 @22:43:12.39 delay 3 seconds @22:43:15.42 PBV.dial between PSV and waste1 @22:43:15.78 PS.seek 0ml @22:43:31.93 delay 2:00 @22:44:09.12 CSV.dial :air @22:44:09.59 CS.reconfigure :fast @22:44:09.64 CS.seek 10ml @22:44:34.37 Pumping wcr puck from tube 4 up to 35psi... @22:44:34.38 CBV.dial SSV @22:44:35.67 CBV.dial between CSV and waste2b @22:44:36.64 CSV.dial between air and CTV @22:44:36.96 CS.seek 7.205ml @22:44:44.21 CTV.dial CSV,avoiding: :air @22:44:44.24 CSV.dial CTV @22:44:45.09 CS.reconfigure :pressurize @22:44:45.14 CS.seek 0ml @22:44:58.32 CBV.dial :waste2b @22:44:58.66 CS.reconfigure :default @22:44:58.71 CS.seek 0ml @22:45:05.17 delay 1:00 @22:45:31.95 recoverPSRreagent08 @22:45:31.97 PTV.dial :puck @22:45:32.14 PBV.dial :waste1 @22:45:32.50 delay 30 seconds @22:46:02.53 PBV.dial between PSV and waste1 @22:46:02.89 PSR.select :air @22:46:02.91 PSV.dial PSR @22:46:03.68 PS.reconfigure :fast @22:46:03.73 PS.seek 2.5ml @22:46:05.20 CTV.dial :air,avoiding: :intake @22:46:06.52 Fix #1 of 2 (with RNAlater) for 2:30 @22:46:06.54 CSV.dial CSR @22:46:07.09 Pulling 2ml of CSR.RNAlater @22:46:07.11 CSR.select :RNAlater @22:46:07.23 CS.seek 2ml @22:46:13.52 delay 5 seconds @22:46:18.54 CSR.select :air @22:46:18.66 CS.reconfigure :fast @22:46:18.71 CS.seek 10ml @22:46:27.80 PSV.dial PTV @22:46:28.69 delay 5 seconds @22:46:33.71 PSV.dial PSR @22:46:34.45 delay 5 seconds @22:46:38.46 Pumping wcr puck from tube 4 up to 35psi... @22:46:38.48 CBV.dial SSV @22:46:39.47 PSV.dial PTV @22:46:40.31 CBV.dial between CSV and waste2b @22:46:40.38 delay 5 seconds @22:46:41.28 CSV.dial between air and CTV @22:46:41.87 CS.seek 7.157ml @22:46:45.40 PSV.dial :air @22:46:46.19 PS.seek 0ml @22:46:49.31 CTV.dial CSV,avoiding: :air @22:46:49.33 CSV.dial CTV @22:46:50.75 CS.reconfigure :pressurize @22:46:50.81 CS.seek 0ml @22:47:08.97 delay 2:30 @22:47:09.86 washCycleDA #2 of 3 @22:47:09.87 PSR.select :air @22:47:09.89 PSV.dial PSR @22:47:11.23 Pulling 0.5ml of PSR.wash @22:47:11.24 PSR.select :wash @22:47:11.37 PS.reconfigure :default @22:47:11.42 PS.seek 0.5ml @22:47:17.33 delay 5 seconds @22:47:22.35 PSR.select :air @22:47:22.47 PS.seek 2ml @22:47:38.67 PTV.dial :puck @22:47:38.75 PSV.dial PTV @22:47:39.50 PBV.dial :waste1 @22:47:39.92 PS.seek 1.5ml @22:47:45.89 delay 3 seconds @22:47:48.91 PBV.dial between PSV and waste1 @22:47:49.27 PS.seek 0ml @22:48:05.42 delay 2:00 @22:49:39.00 CBV.dial :waste2b @22:49:39.33 CS.reconfigure :default @22:49:39.38 CS.seek 0ml @22:49:40.76 delay 1:00 @22:50:05.45 recoverPSRreagent08 @22:50:05.46 PTV.dial :puck @22:50:05.53 PBV.dial :waste1 @22:50:05.90 delay 30 seconds @22:50:35.93 PBV.dial between PSV and waste1 @22:50:36.31 PSR.select :air @22:50:36.33 PSV.dial PSR @22:50:37.10 PS.reconfigure :fast @22:50:37.14 PS.seek 2.5ml @22:50:40.78 CTV.dial :air,avoiding: :intake @22:50:42.11 Fix #2 of 2 (with RNAlater) for 2:30 @22:50:42.13 CSV.dial CSR @22:50:42.58 Pulling 2ml of CSR.RNAlater @22:50:42.59 CSR.select :RNAlater @22:50:42.71 CS.seek 2ml @22:50:48.76 delay 5 seconds @22:50:53.78 CSR.select :air @22:50:53.90 CS.reconfigure :fast @22:50:53.95 CS.seek 10ml @22:51:01.14 PSV.dial PTV @22:51:01.90 delay 5 seconds @22:51:06.92 PSV.dial PSR @22:51:07.67 delay 5 seconds @22:51:12.69 PSV.dial PTV @22:51:13.47 delay 5 seconds @22:51:13.70 Pumping wcr puck from tube 4 up to 35psi... @22:51:13.72 CBV.dial SSV @22:51:14.97 CBV.dial between CSV and waste2b @22:51:15.95 CSV.dial between air and CTV @22:51:16.55 CS.seek 7.173ml @22:51:18.48 PSV.dial :air @22:51:19.25 PS.seek 0ml @22:51:23.92 CTV.dial CSV,avoiding: :air @22:51:23.95 CSV.dial CTV @22:51:25.33 CS.reconfigure :pressurize @22:51:25.38 CS.seek 0ml @22:51:42.92 washCycleDA #3 of 3 @22:51:42.93 PSR.select :air @22:51:42.95 PSV.dial PSR @22:51:43.16 delay 2:30 @22:51:44.28 Pulling 0.5ml of PSR.wash @22:51:44.29 PSR.select :wash @22:51:44.40 PS.reconfigure :default @22:51:44.45 PS.seek 0.5ml @22:51:50.48 delay 5 seconds @22:51:55.50 PSR.select :air @22:51:55.62 PS.seek 2ml @22:52:11.70 PTV.dial :puck @22:52:11.77 PSV.dial PTV @22:52:12.65 PBV.dial :waste1 @22:52:13.09 PS.seek 1.5ml @22:52:19.04 delay 3 seconds @22:52:22.06 PBV.dial between PSV and waste1 @22:52:22.44 PS.seek 0ml @22:52:38.76 delay 2:00 @22:54:13.18 CBV.dial :waste2b @22:54:13.52 CS.reconfigure :default @22:54:13.57 CS.seek 0ml @22:54:15.32 delay 1:00 @22:54:38.79 recoverPSRreagent08 @22:54:38.80 PTV.dial :puck @22:54:38.88 PBV.dial :waste1 @22:54:39.26 delay 30 seconds @22:55:09.29 PBV.dial between PSV and waste1 @22:55:09.66 PSR.select :air @22:55:10.09 PSV.dial PSR @22:55:10.92 PS.reconfigure :fast @22:55:10.97 PS.seek 2.5ml @22:55:15.34 CTV.dial :air,avoiding: :intake @22:55:35.03 PSV.dial PTV @22:55:35.93 delay 5 seconds @22:55:40.95 PSV.dial PSR @22:55:41.71 delay 5 seconds @22:55:46.73 PSV.dial PTV @22:55:47.51 delay 5 seconds @22:55:52.53 PSV.dial :air @22:55:53.42 PS.seek 0ml @22:56:16.69 Gate.power :camera,:ON @22:56:16.71 Mixing DA Substrates @22:56:16.72 PSR.select :air @22:56:16.73 PSV.dial PSR @22:56:18.26 Pulling 0.25ml of PSR.sub1 @22:56:18.29 PSR.select :sub1 @22:56:18.49 PS.reconfigure :default @22:56:18.60 PS.seek 0.25ml @22:56:22.04 delay 5 seconds @22:56:27.06 Pulling 0.25ml of PSR.sub2 @22:56:27.07 PSR.select :sub2 @22:56:27.19 PS.seek 0.5ml @22:56:30.54 delay 5 seconds @22:56:35.56 PSR.select :air @22:56:35.67 PS.seek 2ml @22:56:51.88 PTV.dial :puck @22:56:52.01 PSV.dial PTV @22:56:52.76 PBV.dial :waste1 @22:56:53.21 PS.seek 1.5ml @22:56:59.19 delay 3 seconds @22:57:02.21 PBV.dial between PSV and waste1 @22:57:02.59 PS.seek 0ml @22:57:18.69 delay 10 seconds @22:57:28.72 recoverPSRreagent08 @22:57:28.73 PTV.dial :puck @22:57:28.80 PBV.dial :waste1 @22:57:29.18 delay 30 seconds @22:57:59.21 PBV.dial between PSV and waste1 @22:57:59.58 PSR.select :air @22:57:59.60 PSV.dial PSR @22:58:00.37 PS.reconfigure :fast @22:58:00.42 PS.seek 2.5ml @22:58:24.66 PSV.dial PTV @22:58:25.55 delay 5 seconds @22:58:30.57 PSV.dial PSR @22:58:31.33 delay 5 seconds @22:58:36.35 PSV.dial PTV @22:58:37.13 delay 5 seconds @22:58:42.15 PSV.dial :air @22:58:43.04 PS.seek 0ml @22:59:06.97 Additional air clear @22:59:06.98 PSR.select :air @22:59:07.00 PSV.dial PSR @22:59:08.36 Pulling 2ml of PSR.air @22:59:08.37 PSR.select :air @22:59:08.39 PS.reconfigure :default @22:59:08.44 PS.seek 2ml @22:59:29.75 delay 5 seconds @22:59:34.77 PTV.dial :puck @22:59:34.85 PSV.dial PTV @22:59:35.63 PBV.dial :waste1 @22:59:36.08 PS.seek 1.5ml @22:59:42.03 delay 3 seconds @22:59:45.50 PBV.dial between PSV and waste1 @22:59:45.91 delay 1 second @22:59:46.93 PSV.dial :waste1 @22:59:47.99 PS.seek 0ml @23:00:04.24 Processing Puck Heater at 27.9C before imaging @23:00:04.25 PH.coast @23:00:04.37 Imaging da puck in Processing Clamp while storing it to tube 3 @23:00:04.55 SC.reconfigure :tight @23:00:04.60 SC.seek 3 @23:00:08.18 SC.configure :limp @23:00:08.40 Elbow.reconfigure :tight @23:00:08.45 Elbow.seek 3 @23:00:10.22 Forearm.reconfigure :tight @23:00:10.28 Forearm.seek 3 + 450 counts @23:00:11.31 SE.reconfigure :pick @23:00:11.37 SE.seek :beamBreak @23:00:13.45 SE.configure :default @23:00:13.61 Elbow.reconfigure :limp @23:00:13.66 Forearm.reconfigure :loose @23:00:13.71 Forearm.seek 3 @23:00:14.01 Elbow.hold @23:00:14.09 Elbow.reconfigure :tight @23:00:14.20 SE.seek 15.12 Pucks below Top Plate @23:00:14.65 Forearm.force -20 @23:00:14.70 Forearm.seek 3 + 312 counts @23:00:14.99 Forearm.reconfigure :tight @23:00:15.04 Forearm.seek 3 + 450 counts @23:00:15.24 SE.seek 16.12 Pucks below Top Plate @23:00:15.80 Forearm.reconfigure :loose @23:00:15.85 Forearm.seek :retract @23:00:16.80 Elbow.seek PC @23:00:19.30 Elbow.reconfigure :loose @23:00:19.40 Forearm.seek PC @23:00:20.82 Hand.close @23:00:21.61 PC.seek :open @23:00:26.43 Forearm.seek :retract @23:00:27.41 Elbow.reconfigure :tight @23:00:27.46 Elbow.seek 3 @23:00:30.07 Forearm.reconfigure :tight @23:00:30.13 Forearm.seek 3 + 450 counts @23:00:31.11 Hand.open @23:00:31.81 SE.seek 15.77 Pucks below Top Plate @23:00:32.07 SE.reconfigure :seal @23:00:32.13 SE.seek 14.81 Pucks below Top Plate @23:00:32.65 Lights, camera, ACTION! @23:00:32.69 PuckCamera: snap -bin=2,2 -auto /var/log/esp/da23nov2823h475ml.tif @23:00:52.19 Waiting >20 seconds for da to relinquish FlushPuck @23:00:53.51 SX HX9/16 (USB:08): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 17.526 seconds /var/log/esp/da23nov2823h475ml.tif: TIFF Upload Complete @23:00:53.54 PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da23nov2823h475ml15s.tif @23:01:09.19 SX HX9/16 (USB:08): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15 seconds /var/log/esp/midres/da23nov2823h475ml15s.tif: TIFF Upload Complete @23:01:09.22 PuckCamera: snap -bin=2,2 20.0 /var/log/esp/midres/da23nov2823h475ml20s.tif @23:01:29.87 SX HX9/16 (USB:08): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 20 seconds /var/log/esp/midres/da23nov2823h475ml20s.tif: TIFF Upload Complete @23:01:29.88 Gate.power :camera,:OFF @23:01:29.91 DAfans.email "17.526 sec auto-exposure",Subject: "da23nov2823h475ml images!" @23:01:30.34 Elbow.seek 3 @23:01:30.56 Forearm.seek 3 + 450 counts @23:01:30.67 SE.reconfigure :gently @23:01:30.73 SE.seek 15.52 Pucks below Top Plate @23:01:31.40 Elbow.reconfigure :limp @23:01:31.45 Forearm.reconfigure :loose @23:01:31.53 Forearm.seek 3 @23:01:31.78 Elbow.hold @23:01:31.86 Elbow.reconfigure :tight @23:01:31.92 SE.reconfigure :default @23:01:31.99 SE.seek 15.71 Pucks below Top Plate @23:01:32.43 Forearm.force -20 @23:01:32.47 Forearm.seek 3 + 303 counts @23:01:32.75 Forearm.reconfigure :tight @23:01:33.63 Forearm.seek 3 + 450 counts @23:01:33.98 SE.seek :down @23:01:35.25 Forearm.reconfigure :loose @23:01:35.35 Forearm.seek :retract @23:01:35.66 SE.stop @23:01:36.49 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @23:01:37.07 Elbow.seek FlushPuck::Garage @23:01:38.98 Elbow.reconfigure :loose @23:01:39.20 Forearm.seek :garage @23:01:40.22 Hand.close @23:01:41.15 Forearm.seek :retract @23:01:42.20 Elbow.reconfigure :tight @23:01:42.39 Elbow.seek PC @23:01:43.76 Elbow.reconfigure :loose @23:01:43.97 Forearm.reconfigure :tight @23:01:44.06 Forearm.seek PC @23:01:45.63 PC.seek :closed @23:01:50.43 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.64 @23:01:50.60 Hand.open @23:01:51.39 Forearm.reconfigure :loose @23:01:51.48 Forearm.seek :retract @23:01:52.50 PSR.select :air @23:01:52.58 Storing wcr puck in Collection Clamp to tube 3 @23:01:52.64 PSV.dial PSR @23:01:54.08 Pulling 0.5ml of PSR.flush @23:01:54.16 PSR.select :flush @23:01:54.67 PS.seek 0.5ml @23:01:55.06 Elbow.reconfigure :tight @23:01:55.16 Elbow.seek 3 @23:01:57.97 Forearm.reconfigure :tight @23:01:58.07 Forearm.seek 3 + 450 counts @23:01:59.27 SE.reconfigure :pick @23:01:59.38 SE.seek :beamBreak @23:02:00.68 delay 5 seconds @23:02:01.60 SE.configure :default @23:02:01.93 Elbow.reconfigure :limp @23:02:02.09 Forearm.reconfigure :loose @23:02:02.22 Forearm.seek 3 @23:02:02.57 Elbow.hold @23:02:02.70 Elbow.reconfigure :tight @23:02:02.92 SE.seek 16.12 Pucks below Top Plate @23:02:03.66 Forearm.force -20 @23:02:03.74 Forearm.seek 3 + 311 counts @23:02:04.23 Forearm.reconfigure :tight @23:02:04.33 Forearm.seek 3 + 450 counts @23:02:04.59 SE.seek 17.12 Pucks below Top Plate @23:02:05.49 Forearm.reconfigure :loose @23:02:05.59 Forearm.seek :retract @23:02:05.71 PSR.select :air @23:02:05.89 PS.seek 2.5ml @23:02:06.58 Elbow.seek CC @23:02:07.35 upsync @23:02:08.59 Elbow.reconfigure :loose @23:02:08.76 Forearm.seek CC @23:02:09.76 Hand.close @23:02:10.36 CC.seek :open @23:02:15.16 Forearm.seek :retract @23:02:16.19 Elbow.reconfigure :tight @23:02:16.24 Elbow.seek 3 @23:02:18.02 Elbow.reconfigure :limp @23:02:18.07 Forearm.seek 3 @23:02:19.10 Elbow.hold @23:02:19.17 Elbow.reconfigure :tight @23:02:19.22 Hand.open @23:02:19.98 Forearm.force -20 @23:02:20.03 Forearm.seek 3 + 319 counts @23:02:20.43 Forearm.reconfigure :tight @23:02:20.49 Forearm.seek 3 + 450 counts @23:02:20.88 SE.seek :down @23:02:22.01 Forearm.reconfigure :loose @23:02:22.07 Forearm.seek :retract @23:02:22.43 SE.stop @23:02:23.01
Starting finalPurge @23:02:27.12 delay 1 second @23:02:28.14 PRV.dial :waste2,avoiding: :ab2 @23:02:30.01 PTV.dial :PRVmixing @23:02:31.33 PSV.dial :mixloop @23:02:32.38 PS.seek :empty @23:02:42.48
Waiting >20 seconds for psFlush to relinquish FlushPuck @23:02:58.68 PSR.select :air @23:02:58.70 PSV.dial PSR @23:02:59.75 Pulling 0.5ml of PSR.flush @23:02:59.76 PSR.select :flush @23:03:00.33 PS.seek 0.5ml @23:03:06.25 delay 5 seconds @23:03:11.27 PSR.select :air @23:03:11.40 PS.seek 2.5ml @23:03:32.59 delay 1 second @23:03:33.61 PBV.dial :waste1 @23:03:33.97 PTV.dial :mixing @23:03:34.72 PSV.dial :mixloop @23:03:35.89 PS.seek :empty @23:04:02.21 PSV.dial PSR @23:04:03.25 PSR.select :flush @23:04:03.37 PS.seek 1ml @23:04:14.74 PSR.select :air @23:04:14.87 PS.seek 2.5ml @23:04:31.03 PSV.dial PTV @23:04:31.79 PTV.dial :puck @23:04:32.54 PBV.dial :waste1 @23:04:32.56 PS.seek 0ml @23:04:58.93 PSV.dial PSR @23:04:59.70 PSR.select :flush @23:04:59.82 PS.seek 0.5ml @23:05:05.71 PSR.select :air @23:05:05.83 PS.seek 2.5ml @23:05:27.12 PSV.dial PTV @23:05:27.89 PBV.dial PSV @23:05:28.45 PS.seek 0ml @23:05:54.77 PSV.dial PBV @23:05:55.25 PS.seek 2.5ml @23:06:21.52 PSV.dial :waste1 @23:06:22.27 PS.seek 0ml @23:06:48.62 PSV.dial PSR @23:06:49.09 PSR.select :air @23:06:49.11 PS.seek 2.5ml @23:07:15.55 PSV.dial PTV @23:07:16.29 PBV.dial PSV @23:07:16.48 PS.seek 0ml @23:07:42.80 PSV.dial PBV @23:07:43.28 PS.seek 2.5ml @23:08:09.58 PSV.dial :waste1 @23:08:10.33 PS.seek 0ml @23:08:36.77 PSV.dial PSR @23:08:37.24 PSR.select :flush @23:08:37.37 PS.seek 1ml @23:08:48.49 PSR.select :air @23:08:48.61 PS.seek 2.5ml @23:09:05.18 PSV.dial PTV @23:09:05.91 PTV.dial :puck @23:09:05.99 PBV.dial :waste1 @23:09:06.54 PS.seek 0ml @23:09:33.05 PSV.dial PSR @23:09:33.80 PSR.select :flush @23:09:33.93 PS.seek 0.5ml @23:09:39.96 PSR.select :air @23:09:40.08 PS.seek 2.5ml @23:10:01.36 PSV.dial PTV @23:10:02.13 PBV.dial PSV @23:10:02.70 PS.seek 0ml @23:10:29.27 PSV.dial PBV @23:10:29.75 PS.seek 2.5ml @23:10:56.14 PSV.dial :waste1 @23:10:56.90 PS.seek 0ml @23:11:23.30 PSV.dial PSR @23:11:23.77 PSR.select :air @23:11:23.80 PS.seek 2.5ml @23:11:50.11 PSV.dial PTV @23:11:50.86 PBV.dial PSV @23:11:51.06 PS.seek 0ml @23:12:17.36 PSV.dial PBV @23:12:17.84 PS.seek 2.5ml @23:12:44.20 PSV.dial :waste1 @23:12:44.96 PS.seek 0ml @23:13:11.46
Loading FlushPuck into Collection Clamp from Processing Clamp @23:13:11.65 Elbow.seek PC @23:13:14.14 Elbow.reconfigure :loose @23:13:14.24 Forearm.seek PC @23:13:15.22 Hand.close @23:13:15.85 PC.seek :open @23:13:20.57 Forearm.seek :retract @23:13:21.58 Elbow.reconfigure :tight @23:13:21.63 Elbow.seek CC @23:13:25.25 Elbow.reconfigure :loose @23:13:25.34 Forearm.reconfigure :tight @23:13:25.39 Forearm.seek CC @23:13:27.24 CC.seek :closed @23:13:31.77 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.39 @23:13:31.84 Hand.open @23:13:32.53 Forearm.reconfigure :loose @23:13:32.58 Forearm.seek :retract @23:13:33.53 Flushing the Collection Valve::Manifold @23:13:33.67 CSV.dial CSR @23:13:34.10 Pulling 5ml of CSR.flush @23:13:34.11 CSR.select :flush @23:13:34.23 CS.seek 5ml @23:13:48.11 delay 5 seconds @23:13:53.13 CSR.select :air @23:13:53.30 CS.reconfigure :fast @23:13:53.35 CS.seek 7.499ml @23:14:00.11 CSV.dial :waste @23:14:01.42 CS.reconfigure :default @23:14:01.46 CS.seek 0ml @23:14:21.49 Flushing lower manifold's waste1 port @23:14:21.61 CSV.dial :flush @23:14:22.06 CS.reconfigure :fast @23:14:22.11 CS.seek 5ml @23:14:34.83 delay 5 seconds @23:14:39.86 CSV.dial :air @23:14:40.38 CS.seek 7.001ml @23:14:46.05 CSV.dial CTV @23:14:46.07
CTV.dial CSV @23:14:47.46 CBV.dial :waste1 @23:14:48.23 CS.seek 0ml @23:15:04.83 Flushing lower manifold's waste2 port @23:15:04.96 CSV.dial :flush @23:15:05.68 CS.seek 5ml @23:15:18.33 delay 5 seconds @23:15:23.36 CSV.dial :air @23:15:23.89 CS.seek 7ml @23:15:29.89 CSV.dial CTV @23:15:29.91
CTV.dial CSV @23:15:30.43 CBV.dial :waste2 @23:15:31.48 CS.seek 0ml @23:15:48.22 Flushing lower manifold's waste2b port @23:15:48.72 CSV.dial :flush @23:15:49.54 CS.seek 5ml @23:16:02.24 delay 5 seconds @23:16:07.26 CSV.dial :air @23:16:07.78 CS.seek 7ml @23:16:13.27 CSV.dial CTV @23:16:13.29
CTV.dial CSV @23:16:13.74 CBV.dial :waste2b @23:16:14.81 CS.seek 0ml @23:16:31.33 Disinfecting external Intake with kill @23:16:31.46 CSV.dial CSR @23:16:31.91 Pulling 8ml of CSR.kill @23:16:31.92 CSR.select :kill @23:16:32.03 CS.seek 8ml @23:16:51.92 delay 5 seconds @23:16:56.94 CSR.select :air @23:16:57.06 CS.seek 10ml @23:17:02.68 CSV.dial CBV @23:17:02.70
CBV.dial CSV @23:17:03.78 CTV.dial :intake @23:17:04.56 Intake.select :open @23:17:04.68 CS.seek 2ml @23:17:23.55 Intake.select :close @23:17:23.65 CTV.dial :air @23:17:24.40 CS.reconfigure :default @23:17:24.44 CS.seek 0ml @23:17:30.36 CSV.dial CSR @23:17:31.38 Pulling 5ml of CSR.flush @23:17:31.39 CSR.select :flush @23:17:31.50 CS.seek 5ml @23:17:45.11 delay 5 seconds @23:17:50.13 CSR.select :air @23:17:50.25 CS.seek 10ml @23:18:03.83 CSV.dial CBV @23:18:04.84 CS.seek 0ml @23:18:31.24 Garaging FlushPuck in Collection Clamp @23:18:31.31 Elbow.reconfigure :tight @23:18:31.36 Elbow.seek CC @23:18:31.62 Elbow.reconfigure :loose @23:18:31.72 Forearm.seek CC @23:18:32.73 Hand.close @23:18:33.34 CC.seek :open @23:18:38.04 Forearm.seek :retract @23:18:39.03 Elbow.reconfigure :tight @23:18:39.08 Elbow.seek :garage @23:18:42.04 Elbow.reconfigure :loose @23:18:42.14 Forearm.reconfigure :tight @23:18:42.19 Forearm.seek :garage @23:18:43.19 Hand.open @23:18:43.90 Forearm.reconfigure :loose @23:18:43.95 Forearm.seek :retract @23:18:44.94 Completed [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] begun 21:02:29 @23:18:44.99 Asleep after completing DA_WCR_112823b assays @23:18:45.01 GoodNews.email "{1 => 33,\n 2 => 33,\n 3 => 18,\n 4 => 15,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@23:11:52, 30% flow, 17.3C, 29% humidity, 15.2psia, 13.734V, 0.327A, 104.049Ah, 4.49W\nSampled 400.0ml",Subject: "Asleep after completing DA_WCR_112823b assays" @23:18:49.27 upsync + ESPwhich DA_WCR_112923.rb + fn=DA_WCR_112923.rb + IFS=: + set /home/esp/esp2/mission . + fullPath=/home/esp/esp2/mission/DA_WCR_112923.rb + [ -f /home/esp/esp2/mission/DA_WCR_112923.rb ] + realpath /home/esp/esp2/mission/DA_WCR_112923.rb + return 0 + scriptPath=/home/esp/esp2local/mission/DA_WCR_112923.rb + exec ruby /home/esp/esp2/lib/esp.rb /home/esp/esp2local/mission/DA_WCR_112923.rb @15:38:22.55UTC29-Nov-23
ESP2 v3.42 I2C Gateway v3.20 for core bus @15:38:25.23 Configuration for short puck jake 1/11/21 by brent@mbari.org /home/esp/esp2/type/shallow/jake/configure.rb @15:38:28.86 Polling Can every 10:00 @15:38:28.95 Cleanly exited at 15:34:18UTC29-Nov-23 @15:38:29.00 Loading /home/esp/esp2local/mission/DA_WCR_112923.rb mission @15:38:29.06 GoodNews.email "Starting DA_WCR_112923 w/pucks in tube 4\n{1 => 33,\n 2 => 33,\n 3 => 18,\n 4 => 15,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@15:38:28, 31% flow, 16.6C, 28% humidity, 15.1psia, 13.754V, 0.230A, 107.751Ah, 3.16W",Subject: "DA_WCR_112923 begun" @15:38:29.09
Preparing to sleep until + 00:00:05 @15:38:29.14 Asleep til + 00:00:05 @15:38:29.16 Continuing at 15:38 @15:38:29.44 Gate.power :core,:OFF @15:38:29.46 Gate.power :raw,:OFF @15:38:29.47 Shutdown @15:38:32.08 GoodNews.email "Then, in tube 4: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_112923.rb:14\n{1 => 33,\n 2 => 33,\n 3 => 18,\n 4 => 15,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@15:38:29, 31% flow, 16.5C, 28% humidity, 15.1psia, 13.759V, 0.230A, 107.751Ah, 3.16W",Subject: "Asleep until 15:38" @15:38:33.48 upsync @15:38:34.04
Powering core bus @15:38:36.27 upsync @15:38:39.74
Gate.power :raw,:ON @15:38:40.37 CS.configure :default @15:38:40.42 PS.configure :default @15:38:40.47 SS.configure :default @15:38:40.52 CC.configure :default @15:38:40.56 PC.configure :default @15:38:40.63 Forearm.configure :loose @15:38:40.68 Elbow.configure :loose @15:38:40.75 SC.configure :loose @15:38:40.79 SE.configure :default @15:38:40.84 CH.configure :default @15:38:40.87 PH.configure :default @15:38:40.91 All dwarf controllers running firmware version 3.85 @15:38:41.02 Valve.reset @15:38:41.29 Intake.select :close @15:38:41.34 Exhaust.select :close @15:38:41.36 CTV.dial :air @15:38:41.43 PSV.dial :air @15:38:41.57 CSV.dial :waste1 @15:38:41.59
Forearm.home.seek :retract @15:38:42.05 SSV.dial :spit,avoiding: :exhaust @15:38:42.38 Hand.close @15:38:42.42
Elbow.reconfigure :loose @15:38:42.52 Elbow.jog 1500 @15:38:42.65 PS.home.seek 0ml @15:38:42.93 CS.reconfigure :fast @15:38:42.96 SS.home.seek 0ml @15:38:43.06 CS.home.seek 0ml @15:38:43.15
Elbow.reconfigure :loose @15:38:43.20 Elbow.home.seek :clear @15:38:46.51 SE.home.seek :down @15:38:47.15 SC.reconfigure :tight @15:38:47.20 SC.home.seek :initial @15:38:50.91 PS.stop @15:39:02.98
SC.configure :limp @15:39:03.04 Forearm.seek :retract @15:39:03.10 Elbow.seek :hammer @15:39:04.13 Forearm.reconfigure :tight @15:39:04.18 Forearm.seek retracted @15:39:04.44 PC.home.seek :open @15:39:04.78
CC.home.seek :open @15:39:08.22 Phase [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] @15:39:08.24 Notice.email "At 15:39:08 began in tube 4: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_112923.rb:14\n{1 => 33,\n 2 => 33,\n 3 => 18,\n 4 => 15,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@15:39:08, 31% flow, 16.5C, 28% humidity, 15.1psia, 13.671V, 0.302A, 107.755Ah, 4.14W",Subject: "Started DA_WCR_112923 phase initialPurge; da [1000, 1000] #da and wsr are run simultanelously" @15:39:08.27
Starting initialPurge @15:39:08.28 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @15:39:08.35 Hand.open @15:39:09.11 Elbow.reconfigure :tight @15:39:09.16 Elbow.seek FlushPuck::Garage @15:39:11.13 Elbow.reconfigure :loose @15:39:11.25 Forearm.reconfigure :loose @15:39:11.29 Forearm.seek :garage @15:39:11.99 upsync @15:39:12.27
Hand.close @15:39:12.97 Forearm.seek :retract @15:39:13.92 Elbow.reconfigure :tight @15:39:13.96 Elbow.seek CC @15:39:16.97 Elbow.reconfigure :loose @15:39:17.07 Forearm.reconfigure :tight @15:39:17.12 Forearm.seek CC @15:39:18.39 CC.seek :closed @15:39:23.00 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.40 @15:39:23.06 Hand.open @15:39:23.78 Forearm.reconfigure :loose @15:39:23.83 Forearm.seek :retract @15:39:24.88 Flushing the Collection Valve::Manifold @15:39:25.01 CSV.dial CSR @15:39:26.30 Pulling 5ml of CSR.flush @15:39:26.31 CSR.select :flush @15:39:26.43 CS.reconfigure :default @15:39:26.89 CS.seek 5ml @15:39:40.71 delay 5 seconds @15:39:45.72 CSR.select :air @15:39:45.89 CS.reconfigure :fast @15:39:45.94 CS.seek 7.5ml @15:39:52.80 CSV.dial :waste @15:39:54.09 CS.reconfigure :default @15:39:54.13 CS.seek 0ml @15:40:14.14 Purging external Intake @15:40:14.26 CSV.dial :flush @15:40:14.71 CS.reconfigure :fast @15:40:14.76 CS.seek 10ml @15:40:39.24 delay 5 seconds @15:40:44.26 CSV.dial CBV @15:40:44.31
CBV.dial CSV @15:40:45.03 CTV.dial :intake @15:40:45.83 Intake.select :open @15:40:45.94 CS.seek 0ml @15:41:09.36 Intake.select :close @15:41:09.46 CTV.dial :air @15:41:10.29 Flushing upper manifold @15:41:10.46 CSV.dial :flush @15:41:11.20 CS.seek 5ml @15:41:23.89 delay 5 seconds @15:41:28.91 CSV.dial :air @15:41:29.42 CS.seek 7ml @15:41:35.14 CSV.dial CBV @15:41:35.16
CBV.dial CSV @15:41:36.22 CTV.dial :air @15:41:36.30 CS.seek 0ml @15:41:52.99 delay 7 seconds @15:42:00.01 CS.seek 3ml @15:42:08.08 CSV.dial :waste @15:42:08.54 CS.seek 0ml @15:42:16.31 CSV.dial :flush @15:42:16.76 CS.seek 3ml @15:42:24.83 delay 5 seconds @15:42:29.85 CSV.dial :air @15:42:30.36 CS.seek 5ml @15:42:36.11 CSV.dial :air @15:42:36.29 CS.seek 0ml @15:42:48.42 delay 7 seconds @15:42:55.45 CS.seek 3ml @15:43:03.58 CSV.dial :waste @15:43:04.32 CS.seek 0ml @15:43:12.08 Garaging FlushPuck in Collection Clamp @15:43:12.15 Elbow.reconfigure :tight @15:43:12.20 Elbow.seek CC @15:43:12.58 Elbow.reconfigure :loose @15:43:12.68 Forearm.seek CC @15:43:13.69 Hand.close @15:43:14.35 CC.seek :open @15:43:19.45 Forearm.seek :retract @15:43:20.44 Elbow.reconfigure :tight @15:43:20.49 Elbow.seek :garage @15:43:23.37 Elbow.reconfigure :loose @15:43:23.47 Forearm.reconfigure :tight @15:43:23.52 Forearm.seek :garage @15:43:24.53 Hand.open @15:43:25.16 Forearm.reconfigure :loose @15:43:25.20 Forearm.seek :retract @15:43:26.15 da sampling at most 1000ml, wcr at most 1000ml @15:43:26.19 Loading sh1 puck from tube 4 into Collection Clamp @15:43:26.20 DAfans.email "Started da in tube 4 at 15:43:26UTC29-Nov-23\n{1 => 33,\n 2 => 33,\n 3 => 18,\n 4 => 15,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@15:39:08, 31% flow, 16.5C, 28% humidity, 15.1psia, 13.671V, 0.302A, 107.755Ah, 4.14W",Subject: "da sampling at most 1000ml, wcr at most 1000ml" @15:43:26.73
Elbow.reconfigure :tight @15:43:26.78 Elbow.seek 4 @15:43:28.86 Forearm.reconfigure :tight @15:43:28.98 Forearm.seek 4 + 450 counts @15:43:30.19 SE.reconfigure :pick @15:43:30.25 SE.seek :beamBreak @15:43:30.29 upsync @15:43:32.50
SE.configure :default @15:43:32.67 Elbow.seek 4 @15:43:32.86 Forearm.seek 4 + 300 counts @15:43:33.02 Forearm.reconfigure :loose @15:43:33.07 Hand.close @15:43:33.74 SE.seek :down @15:43:33.94
Forearm.seek :retract @15:43:35.28 Elbow.seek CC @15:43:35.54 SE.stop @15:43:36.94
Elbow.reconfigure :loose @15:43:37.04 Forearm.reconfigure :tight @15:43:37.09 Forearm.seek CC @15:43:38.29 CC.seek :closed @15:43:42.86 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.32 @15:43:42.87 Hand.open @15:43:43.53 Forearm.reconfigure :loose @15:43:43.58 Forearm.seek :retract @15:43:44.52 collectSample 1000ml w/bubble point of 24psi @15:43:44.54 Priming 7ml @15:43:44.55 Intake.select :close @15:43:44.56 Exhaust.select :close @15:43:44.57 SS.hold @15:43:44.77 Calibrating pressure and temperature sensors @15:43:44.80 CTV.dial :air @15:43:44.88 SSV.dial :spit,avoiding: :exhaust @15:43:44.96 SS.reconfigure :fast @15:43:45.00 SS.seek 5ml @15:43:47.89 SSV.dial CBV @15:43:47.91
CBV.dial SSV @15:43:49.26 delay 3.8 seconds @15:43:53.19 Sensor limits: Intake max 103.7psia; Outlet max 96.1psia @15:43:53.20 SSV.dial :spit @15:43:54.53 SS.seek 0ml @15:43:57.37 SSV.dial CBV @15:43:58.68 CTV.dial :intake @15:43:59.47 SSV.dial CBV @15:43:59.49
CBV.dial SSV @15:43:59.58 Allowing 40s for 7.0ml of remaining 7.0ml @15:43:59.59 Intake.select :open @15:44:01.75 Intake @14.4psia, vacuum from 8.9 to 11.9psi @15:44:01.76 SS.reconfigure :inhaling 2.20ml/s @15:44:01.81 SS.seek 7ml @15:44:08.63 Stroke completed -- waiting for pressure to equalize ... @15:44:08.64 SS.reconfigure :exhale @15:44:08.69 SS.hold @15:44:15.42 Intake.select :close @15:44:15.58 SSV.dial :exhaust @15:44:16.34 Exhaust.select :open @15:44:16.45 SS.seek 0ml @15:44:20.76 Exhaust.select :close @15:44:20.87 Sampling 1000.0ml @15:44:20.88 Intake.select :close @15:44:20.89 Exhaust.select :close @15:44:20.90 SS.reconfigure :default @15:44:20.95 SS.hold @15:44:21.15 CTV.dial :intake @15:44:21.24 SSV.dial CBV @15:44:21.28
CBV.dial SSV @15:44:22.06 Allowing 130s for 25.0ml of remaining 1000.0ml @15:44:22.07 Intake.select :open @15:44:24.23 Intake @15.1psia, vacuum from 9.6 to 12.6psi @15:44:24.25 SS.reconfigure :inhaling 2.20ml/s @15:44:24.30 SS.seek 25ml @15:44:39.02 Stroke completed -- waiting for pressure to equalize ... @15:44:39.03 SS.reconfigure :exhale @15:44:39.08 SS.hold @15:45:08.07 Intake.select :close @15:45:08.22 SSV.dial :exhaust @15:45:08.99 Exhaust.select :open @15:45:09.11 SS.seek 0ml @15:45:17.91 Exhaust.select :close @15:45:18.02 SSV.dial CBV @15:45:18.05
CBV.dial SSV @15:45:18.82 Allowing 130s for 25.0ml of remaining 975.0ml @15:45:18.83 Intake.select :open @15:45:21.00 Intake @15.8psia, vacuum from 10.0 to 13.0psi @15:45:21.01 SS.reconfigure :inhaling 2.20ml/s @15:45:21.06 SS.seek 25ml @15:45:32.11 Vacuum too high -- waiting up to 40 seconds @15:45:32.12 SS.reconfigure :exhale @15:45:32.18 SS.hold @15:45:36.70 Intake @16.8psia, vacuum from 10.0 to 13.0psi @15:45:36.72 SS.reconfigure :inhaling 1.76ml/s @15:45:36.77 SS.seek 25ml @15:45:42.65 Stroke completed -- waiting for pressure to equalize ... @15:45:42.66 SS.reconfigure :exhale @15:45:42.70 SS.hold @15:46:07.42 Intake.select :close @15:46:07.58 SSV.dial :exhaust @15:46:08.35 Exhaust.select :open @15:46:08.47 SS.seek 0ml @15:46:17.46 Exhaust.select :close @15:46:17.57 SSV.dial CBV @15:46:17.59
CBV.dial SSV @15:46:18.37 Allowing 130s for 25.0ml of remaining 950.0ml @15:46:18.38 Intake.select :open @15:46:20.54 Intake @15.8psia, vacuum from 10.0 to 13.0psi @15:46:20.55 SS.reconfigure :inhaling 1.76ml/s @15:46:20.60 SS.seek 25ml @15:46:36.23 Vacuum too high -- waiting up to 40 seconds @15:46:36.25 SS.reconfigure :exhale @15:46:36.30 SS.hold @15:46:39.70 Intake @14.8psia, vacuum from 9.3 to 12.3psi @15:46:39.72 SS.reconfigure :inhaling 1.41ml/s @15:46:39.76 SS.seek 25ml @15:46:41.75 Stroke completed -- waiting for pressure to equalize ... @15:46:41.76 SS.reconfigure :exhale @15:46:41.80 SS.hold @15:47:08.32 Intake.select :close @15:47:08.48 SSV.dial :exhaust @15:47:09.67 Exhaust.select :open @15:47:09.79 SS.seek 0ml @15:47:18.74 Exhaust.select :close @15:47:18.85 SSV.dial CBV @15:47:18.87
CBV.dial SSV @15:47:19.68 Allowing 130s for 25.0ml of remaining 925.0ml @15:47:19.69 Intake.select :open @15:47:21.86 Intake @15.7psia, vacuum from 10.0 to 13.0psi @15:47:21.88 SS.reconfigure :inhaling 1.41ml/s @15:47:21.92 SS.seek 25ml @15:47:42.41 Stroke completed -- waiting for pressure to equalize ... @15:47:42.42 SS.reconfigure :exhale @15:47:42.47 SS.hold @15:48:04.79 Intake.select :close @15:48:04.94 SSV.dial :exhaust @15:48:05.71 Exhaust.select :open @15:48:05.82 SS.seek 0ml @15:48:14.75 Exhaust.select :close @15:48:14.86 SSV.dial CBV @15:48:14.89
CBV.dial SSV @15:48:15.66 Allowing 130s for 25.0ml of remaining 900.0ml @15:48:15.68 Intake.select :open @15:48:18.27 Intake @15.8psia, vacuum from 10.0 to 13.0psi @15:48:18.28 SS.reconfigure :inhaling 1.41ml/s @15:48:18.33 SS.seek 25ml @15:48:38.52 Stroke completed -- waiting for pressure to equalize ... @15:48:38.53 SS.reconfigure :exhale @15:48:38.58 SS.hold @15:48:58.99 Intake.select :close @15:48:59.14 SSV.dial :exhaust @15:48:59.93 Exhaust.select :open @15:49:00.04 SS.seek 0ml @15:49:08.82 Exhaust.select :close @15:49:08.93 SSV.dial CBV @15:49:08.96
CBV.dial SSV @15:49:09.73 Allowing 130s for 25.0ml of remaining 875.0ml @15:49:09.74 Intake.select :open @15:49:11.90 Intake @15.9psia, vacuum from 10.0 to 13.0psi @15:49:11.91 SS.reconfigure :inhaling 1.41ml/s @15:49:11.96 SS.seek 25ml @15:49:32.53 Stroke completed -- waiting for pressure to equalize ... @15:49:32.54 SS.reconfigure :exhale @15:49:32.59 SS.hold @15:49:53.24 Intake.select :close @15:49:53.39 SSV.dial :exhaust @15:49:54.17 Exhaust.select :open @15:49:54.28 SS.seek 0ml @15:50:02.99 Exhaust.select :close @15:50:03.09 SSV.dial CBV @15:50:03.11
CBV.dial SSV @15:50:03.88 Allowing 130s for 25.0ml of remaining 850.0ml @15:50:03.89 Intake.select :open @15:50:06.06 Intake @15.8psia, vacuum from 10.0 to 13.0psi @15:50:06.07 SS.reconfigure :inhaling 1.41ml/s @15:50:06.12 SS.seek 25ml @15:50:26.58 Stroke completed -- waiting for pressure to equalize ... @15:50:26.59 SS.reconfigure :exhale @15:50:26.64 SS.hold @15:50:42.97 Intake.select :close @15:50:43.12 SSV.dial :exhaust @15:50:43.88 Exhaust.select :open @15:50:43.99 SS.seek 0ml @15:50:52.61 Exhaust.select :close @15:50:52.72 SSV.dial CBV @15:50:52.75
CBV.dial SSV @15:50:53.52 Allowing 130s for 25.0ml of remaining 825.0ml @15:50:53.53 Intake.select :open @15:50:55.70 Intake @15.9psia, vacuum from 10.0 to 13.0psi @15:50:55.71 SS.reconfigure :inhaling 1.41ml/s @15:50:55.76 SS.seek 25ml @15:51:16.31 Stroke completed -- waiting for pressure to equalize ... @15:51:16.32 SS.reconfigure :exhale @15:51:16.37 SS.hold @15:51:36.78 Intake.select :close @15:51:36.94 SSV.dial :exhaust @15:51:37.70 Exhaust.select :open @15:51:37.82 SS.seek 0ml @15:51:46.56 Exhaust.select :close @15:51:46.68 SSV.dial CBV @15:51:46.72
CBV.dial SSV @15:51:47.46 Allowing 130s for 25.0ml of remaining 800.0ml @15:51:47.47 Intake.select :open @15:51:49.63 Intake @15.7psia, vacuum from 10.0 to 13.0psi @15:51:49.65 SS.reconfigure :inhaling 1.41ml/s @15:51:49.70 SS.seek 25ml @15:52:10.36 Stroke completed -- waiting for pressure to equalize ... @15:52:10.37 SS.reconfigure :exhale @15:52:10.41 SS.hold @15:52:27.07 Intake.select :close @15:52:27.22 SSV.dial :exhaust @15:52:27.98 Exhaust.select :open @15:52:28.10 SS.seek 0ml @15:52:36.84 Exhaust.select :close @15:52:36.95 SSV.dial CBV @15:52:36.97
CBV.dial SSV @15:52:37.75 Allowing 130s for 25.0ml of remaining 775.0ml @15:52:37.76 Intake.select :open @15:52:39.93 Intake @15.8psia, vacuum from 10.0 to 13.0psi @15:52:39.94 SS.reconfigure :inhaling 1.41ml/s @15:52:39.99 SS.seek 25ml @15:53:00.56 Stroke completed -- waiting for pressure to equalize ... @15:53:00.57 SS.reconfigure :exhale @15:53:00.62 SS.hold @15:53:18.85 Intake.select :close @15:53:19.43 SSV.dial :exhaust @15:53:20.20 Exhaust.select :open @15:53:20.32 SS.seek 0ml @15:53:29.08 Exhaust.select :close @15:53:29.18 SSV.dial CBV @15:53:29.20
CBV.dial SSV @15:53:30.00 Allowing 130s for 25.0ml of remaining 750.0ml @15:53:30.01 Intake.select :open @15:53:32.18 Intake @15.7psia, vacuum from 10.0 to 13.0psi @15:53:32.19 SS.reconfigure :inhaling 1.41ml/s @15:53:32.24 SS.seek 25ml @15:53:52.78 Stroke completed -- waiting for pressure to equalize ... @15:53:52.79 SS.reconfigure :exhale @15:53:52.83 SS.hold @15:54:11.08 Intake.select :close @15:54:11.23 SSV.dial :exhaust @15:54:11.99 Exhaust.select :open @15:54:12.10 SS.seek 0ml @15:54:21.01 Exhaust.select :close @15:54:21.12 SSV.dial CBV @15:54:21.14
CBV.dial SSV @15:54:21.92 Allowing 130s for 25.0ml of remaining 725.0ml @15:54:21.93 Intake.select :open @15:54:24.10 Intake @15.8psia, vacuum from 10.0 to 13.0psi @15:54:24.47 SS.reconfigure :inhaling 1.41ml/s @15:54:24.59 SS.seek 25ml @15:54:44.76 Stroke completed -- waiting for pressure to equalize ... @15:54:44.77 SS.reconfigure :exhale @15:54:44.82 SS.hold @15:55:05.21 Intake.select :close @15:55:05.37 SSV.dial :exhaust @15:55:06.13 Exhaust.select :open @15:55:06.24 SS.seek 0ml @15:55:15.00 Exhaust.select :close @15:55:15.11 SSV.dial CBV @15:55:15.15
CBV.dial SSV @15:55:15.89 Allowing 130s for 25.0ml of remaining 700.0ml @15:55:15.90 Intake.select :open @15:55:18.06 Intake @15.7psia, vacuum from 10.0 to 13.0psi @15:55:18.08 SS.reconfigure :inhaling 1.41ml/s @15:55:18.13 SS.seek 25ml @15:55:38.73 Stroke completed -- waiting for pressure to equalize ... @15:55:38.74 SS.reconfigure :exhale @15:55:38.78 SS.hold @15:55:59.37 Intake.select :close @15:55:59.52 SSV.dial :exhaust @15:56:00.29 Exhaust.select :open @15:56:00.40 SS.seek 0ml @15:56:09.40 Exhaust.select :close @15:56:09.51 SSV.dial CBV @15:56:09.53
CBV.dial SSV @15:56:10.31 Allowing 130s for 25.0ml of remaining 675.0ml @15:56:10.32 Intake.select :open @15:56:12.49 Intake @15.7psia, vacuum from 10.0 to 13.0psi @15:56:12.50 SS.reconfigure :inhaling 1.41ml/s @15:56:12.55 SS.seek 25ml @15:56:32.56 Stroke completed -- waiting for pressure to equalize ... @15:56:32.57 SS.reconfigure :exhale @15:56:32.61 SS.hold @15:56:55.17 Intake.select :close @15:56:55.33 SSV.dial :exhaust @15:56:56.11 Exhaust.select :open @15:56:56.22 SS.seek 0ml @15:57:05.15 Exhaust.select :close @15:57:05.26 SSV.dial CBV @15:57:05.28
CBV.dial SSV @15:57:06.06 Allowing 130s for 25.0ml of remaining 650.0ml @15:57:06.07 Intake.select :open @15:57:08.23 Intake @15.8psia, vacuum from 10.0 to 13.0psi @15:57:08.25 SS.reconfigure :inhaling 1.41ml/s @15:57:08.30 SS.seek 25ml @15:57:26.59 Vacuum too high -- waiting up to 40 seconds @15:57:26.61 SS.reconfigure :exhale @15:57:26.66 SS.hold @15:57:39.58 Intake @17.9psia, vacuum from 10.0 to 13.0psi @15:57:39.59 SS.reconfigure :inhaling 1.13ml/s @15:57:39.64 SS.seek 25ml @15:57:42.41 Stroke completed -- waiting for pressure to equalize ... @15:57:42.42 SS.reconfigure :exhale @15:57:42.47 SS.hold @15:57:52.26 Intake.select :close @15:57:52.41 SSV.dial :exhaust @15:57:53.18 Exhaust.select :open @15:57:53.29 SS.seek 0ml @15:58:02.21 Exhaust.select :close @15:58:02.32 SSV.dial CBV @15:58:02.34
CBV.dial SSV @15:58:03.12 Allowing 130s for 25.0ml of remaining 625.0ml @15:58:03.13 Intake.select :open @15:58:05.30 Intake @15.6psia, vacuum from 10.0 to 13.0psi @15:58:05.31 SS.reconfigure :inhaling 1.13ml/s @15:58:05.36 SS.seek 25ml @15:58:30.09 Stroke completed -- waiting for pressure to equalize ... @15:58:30.10 SS.reconfigure :exhale @15:58:30.14 SS.hold @15:58:50.52 Intake.select :close @15:58:50.68 SSV.dial :exhaust @15:58:51.44 Exhaust.select :open @15:58:51.55 SS.seek 0ml @15:59:00.54 Exhaust.select :close @15:59:00.65 SSV.dial CBV @15:59:00.67
CBV.dial SSV @15:59:01.45 Allowing 130s for 25.0ml of remaining 600.0ml @15:59:01.46 Intake.select :open @15:59:03.62 Intake @15.8psia, vacuum from 10.0 to 13.0psi @15:59:03.63 SS.reconfigure :inhaling 1.13ml/s @15:59:03.68 SS.seek 25ml @15:59:27.12 Vacuum too high -- waiting up to 40 seconds @15:59:27.14 SS.reconfigure :exhale @15:59:27.19 SS.hold @15:59:40.40 Intake @17.5psia, vacuum from 10.0 to 13.0psi @15:59:40.41 SS.reconfigure :inhaling 0.91ml/s @15:59:40.46 SS.seek 25ml @15:59:42.04 Stroke completed -- waiting for pressure to equalize ... @15:59:42.05 SS.reconfigure :exhale @15:59:42.09 SS.hold @15:59:51.01 Intake.select :close @15:59:51.24 SSV.dial :exhaust @15:59:52.01 Exhaust.select :open @15:59:52.13 SS.seek 0ml @16:00:00.87 Exhaust.select :close @16:00:00.97 SSV.dial CBV @16:00:00.99
CBV.dial SSV @16:00:02.60 Allowing 130s for 25.0ml of remaining 575.0ml @16:00:02.61 Intake.select :open @16:00:04.78 Intake @15.7psia, vacuum from 10.0 to 13.0psi @16:00:04.79 SS.reconfigure :inhaling 0.91ml/s @16:00:04.84 SS.seek 25ml @16:00:34.85 Stroke completed -- waiting for pressure to equalize ... @16:00:34.86 SS.reconfigure :exhale @16:00:34.90 SS.hold @16:00:55.23 Intake.select :close @16:00:55.39 SSV.dial :exhaust @16:00:56.15 Exhaust.select :open @16:00:56.26 SS.seek 0ml @16:01:04.99 Exhaust.select :close @16:01:05.10 SSV.dial CBV @16:01:05.52
CBV.dial SSV @16:01:06.34 Allowing 130s for 25.0ml of remaining 550.0ml @16:01:06.35 Intake.select :open @16:01:08.51 Intake @15.8psia, vacuum from 10.0 to 13.0psi @16:01:08.52 SS.reconfigure :inhaling 0.91ml/s @16:01:08.57 SS.seek 25ml @16:01:38.39 Stroke completed -- waiting for pressure to equalize ... @16:01:38.40 SS.reconfigure :exhale @16:01:38.45 SS.hold @16:02:02.99 Intake.select :close @16:02:03.14 SSV.dial :exhaust @16:02:03.91 Exhaust.select :open @16:02:04.03 SS.seek 0ml @16:02:12.99 Exhaust.select :close @16:02:13.09 SSV.dial CBV @16:02:13.13
CBV.dial SSV @16:02:13.88 Allowing 130s for 25.0ml of remaining 525.0ml @16:02:14.25 Intake.select :open @16:02:16.48 Intake @15.6psia, vacuum from 10.0 to 13.0psi @16:02:16.50 SS.reconfigure :inhaling 0.91ml/s @16:02:16.55 SS.seek 25ml @16:02:45.92 Stroke completed -- waiting for pressure to equalize ... @16:02:45.93 SS.reconfigure :exhale @16:02:45.98 SS.hold @16:03:12.51 Intake.select :close @16:03:12.66 SSV.dial :exhaust @16:03:13.42 Exhaust.select :open @16:03:13.53 SS.seek 0ml @16:03:22.33 Exhaust.select :close @16:03:22.44 SSV.dial CBV @16:03:22.46
CBV.dial SSV @16:03:23.31 Allowing 130s for 25.0ml of remaining 500.0ml @16:03:23.32 Intake.select :open @16:03:25.49 Intake @15.7psia, vacuum from 10.0 to 13.0psi @16:03:25.50 SS.reconfigure :inhaling 0.91ml/s @16:03:25.55 SS.seek 25ml @16:03:51.25 Vacuum too high -- waiting up to 40 seconds @16:03:51.26 SS.reconfigure :exhale @16:03:51.31 SS.hold @16:04:07.78 Intake @16.7psia, vacuum from 10.0 to 13.0psi @16:04:07.79 SS.reconfigure :inhaling 0.73ml/s @16:04:07.84 SS.seek 25ml @16:04:12.70 Stroke completed -- waiting for pressure to equalize ... @16:04:12.71 SS.reconfigure :exhale @16:04:12.75 SS.hold @16:04:27.22 Intake.select :close @16:04:27.37 SSV.dial :exhaust @16:04:28.14 Exhaust.select :open @16:04:28.26 SS.seek 0ml @16:04:36.94 Exhaust.select :close @16:04:37.05 SSV.dial CBV @16:04:37.07
CBV.dial SSV @16:04:37.85 Allowing 130s for 25.0ml of remaining 475.0ml @16:04:37.86 Intake.select :open @16:04:40.02 Intake @15.7psia, vacuum from 10.0 to 13.0psi @16:04:40.03 SS.reconfigure :inhaling 0.73ml/s @16:04:40.08 SS.seek 25ml @16:05:16.36 Stroke completed -- waiting for pressure to equalize ... @16:05:16.37 SS.reconfigure :exhale @16:05:16.42 SS.hold @16:05:45.02 Intake.select :close @16:05:45.17 SSV.dial :exhaust @16:05:45.92 Exhaust.select :open @16:05:46.04 SS.seek 0ml @16:05:54.83 Exhaust.select :close @16:05:54.94 SSV.dial CBV @16:05:54.96
CBV.dial SSV @16:05:55.75 Allowing 130s for 25.0ml of remaining 450.0ml @16:05:55.76 Intake.select :open @16:05:57.93 Intake @15.7psia, vacuum from 10.0 to 13.0psi @16:05:57.95 SS.reconfigure :inhaling 0.73ml/s @16:05:58.00 SS.seek 25ml @16:06:28.71 Vacuum too high -- waiting up to 40 seconds @16:06:28.72 SS.reconfigure :exhale @16:06:28.78 SS.hold @16:06:47.25 Intake @17.6psia, vacuum from 10.0 to 13.0psi @16:06:47.26 SS.reconfigure :inhaling 0.59ml/s @16:06:47.31 SS.seek 25ml @16:06:54.62 Stroke completed -- waiting for pressure to equalize ... @16:06:54.63 SS.reconfigure :exhale @16:06:54.68 SS.hold @16:07:11.39 Intake.select :close @16:07:11.54 SSV.dial :exhaust @16:07:12.31 Exhaust.select :open @16:07:12.43 SS.seek 0ml @16:07:21.37 Exhaust.select :close @16:07:21.48 SSV.dial CBV @16:07:21.52
CBV.dial SSV @16:07:22.29 Allowing 130s for 25.0ml of remaining 425.0ml @16:07:22.30 Intake.select :open @16:07:24.46 Intake @15.7psia, vacuum from 10.0 to 13.0psi @16:07:24.47 SS.reconfigure :inhaling 0.59ml/s @16:07:24.52 SS.seek 25ml @16:08:09.12 Stroke completed -- waiting for pressure to equalize ... @16:08:09.13 SS.reconfigure :exhale @16:08:09.18 SS.hold @16:08:39.81 Intake.select :close @16:08:39.96 SSV.dial :exhaust @16:08:40.72 Exhaust.select :open @16:08:40.84 SS.seek 0ml @16:08:49.90 Exhaust.select :close @16:08:50.01 SSV.dial CBV @16:08:50.03
CBV.dial SSV @16:08:50.83 Allowing 130s for 25.0ml of remaining 400.0ml @16:08:50.84 Intake.select :open @16:08:53.00 Intake @15.8psia, vacuum from 10.0 to 13.0psi @16:08:53.01 SS.reconfigure :inhaling 0.59ml/s @16:08:53.06 SS.seek 25ml @16:09:37.43 Stroke completed -- waiting for pressure to equalize ... @16:09:37.44 SS.reconfigure :exhale @16:09:37.49 SS.hold @16:10:14.68 Intake.select :close @16:10:14.84 SSV.dial :exhaust @16:10:15.61 Exhaust.select :open @16:10:15.72 SS.seek 0ml @16:10:24.73 Exhaust.select :close @16:10:24.84 SSV.dial CBV @16:10:24.86
CBV.dial SSV @16:10:25.64 Allowing 130s for 25.0ml of remaining 375.0ml @16:10:25.65 Intake.select :open @16:10:27.81 Intake @15.8psia, vacuum from 10.0 to 13.0psi @16:10:27.83 SS.reconfigure :inhaling 0.59ml/s @16:10:27.88 SS.seek 25ml @16:11:03.39 Vacuum too high -- waiting up to 40 seconds @16:11:03.41 SS.reconfigure :exhale @16:11:03.46 SS.hold @16:11:24.05 Intake @17.3psia, vacuum from 10.0 to 13.0psi @16:11:24.06 SS.reconfigure :inhaling 0.47ml/s @16:11:24.11 SS.seek 25ml @16:11:35.74 Stroke completed -- waiting for pressure to equalize ... @16:11:35.75 SS.reconfigure :exhale @16:11:35.80 SS.hold @16:11:58.22 Intake.select :close @16:11:58.37 SSV.dial :exhaust @16:11:59.12 Exhaust.select :open @16:11:59.66 SS.seek 0ml @16:12:08.45 Exhaust.select :close @16:12:08.55 SSV.dial CBV @16:12:08.59
CBV.dial SSV @16:12:09.34 Allowing 130s for 25.0ml of remaining 350.0ml @16:12:09.35 Intake.select :open @16:12:11.52 Intake @15.6psia, vacuum from 10.0 to 13.0psi @16:12:11.54 SS.reconfigure :inhaling 0.47ml/s @16:12:11.59 SS.seek 25ml @16:13:06.93 Stroke completed -- waiting for pressure to equalize ... @16:13:06.94 SS.reconfigure :exhale @16:13:06.99 SS.hold @16:13:45.76 Intake.select :close @16:13:45.92 SSV.dial :exhaust @16:13:46.70 Exhaust.select :open @16:13:46.82 SS.seek 0ml @16:13:55.66 Exhaust.select :close @16:13:55.77 SSV.dial CBV @16:13:55.79
CBV.dial SSV @16:13:56.57 Allowing 130s for 25.0ml of remaining 325.0ml @16:13:56.58 Intake.select :open @16:13:58.75 Intake @15.7psia, vacuum from 10.0 to 13.0psi @16:13:58.77 SS.reconfigure :inhaling 0.47ml/s @16:13:58.82 SS.seek 25ml @16:14:50.67 Vacuum too high -- waiting up to 40 seconds @16:14:50.68 SS.reconfigure :exhale @16:14:50.74 SS.hold @16:15:17.43 Intake @17.1psia, vacuum from 10.0 to 13.0psi @16:15:17.44 SS.reconfigure :inhaling 0.38ml/s @16:15:17.49 SS.seek 25ml @16:15:22.55 Stroke completed -- waiting for pressure to equalize ... @16:15:22.56 SS.reconfigure :exhale @16:15:22.61 SS.hold @16:15:42.99 Intake.select :close @16:15:43.14 SSV.dial :exhaust @16:15:43.90 Exhaust.select :open @16:15:44.01 SS.seek 0ml @16:15:53.05 Exhaust.select :close @16:15:53.16 SSV.dial CBV @16:15:53.54
CBV.dial SSV @16:15:54.38 Allowing 130s for 25.0ml of remaining 300.0ml @16:15:54.39 Intake.select :open @16:15:56.56 Intake @15.8psia, vacuum from 10.0 to 13.0psi @16:15:56.58 SS.reconfigure :inhaling 0.38ml/s @16:15:56.63 SS.seek 25ml @16:17:05.38 Stroke completed -- waiting for pressure to equalize ... @16:17:05.39 SS.reconfigure :exhale @16:17:05.43 SS.hold @16:17:45.63 SS.reconfigure :default @16:17:45.68 SS.hold @16:17:45.74 Intake.select :close @16:17:45.90 ShallowSampler::Clogged in MAIN -- Intake @17psia & Outlet @14psia won't equalize after 725.0ml @16:17:45.91 SSV.dial :exhaust @16:17:46.68 Exhaust.select :open @16:17:46.79 SS.reconfigure :exhale @16:17:46.83 SS.seek 0ml @16:17:55.83 Exhaust.select :close @16:17:55.94 Sampled 725.0ml @16:17:55.95 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @16:17:55.96 Intake.select :close @16:17:56.03 CSV.dial :waste @16:17:56.19 SSV.dial CBV,avoiding: :exhaust @16:17:56.21 CS.seek 0ml @16:17:56.24
CBV.dial SSV @16:17:56.33 CSV.dial :air @16:17:57.19 CS.seek 10ml @16:17:58.13
CTV.dial :air @16:17:58.92 delay 15 seconds @16:18:13.94 SSV.dial :spit,avoiding: :exhaust @16:18:15.28 CTV.dial CSV,avoiding: :intake @16:18:15.31
SS.seek 0ml @16:18:15.43 SSV.dial CBV,avoiding: :exhaust @16:18:21.80 SSV.dial between CBV and plug @16:18:22.27 CBV.dial between SSV and waste2 @16:18:22.68 CTV.dial CSV,avoiding: :air @16:18:22.70
CSV.dial CTV @16:18:23.21 CS.reconfigure :pressurize @16:18:23.26 CS.seek 0ml @16:18:41.43 BubblePoint: Intake @24.3psia, Outlet @0.8psia @16:18:41.44 CBV.dial SSV @16:18:41.78 SS.reconfigure :default @16:18:41.83 SS.seek 10ml @16:18:47.36 delay 15 seconds @16:19:02.49 Evacuated: Intake @23.6psia, Outlet @23.6psia @16:19:02.50 SSV.dial :spit,avoiding: :exhaust @16:19:03.54 SS.reconfigure :exhale @16:19:03.59 SS.seek 0ml @16:19:08.84 SS.reconfigure :fast @16:19:08.89 SS.seek 5ml @16:19:11.45 SSV.dial CBV,avoiding: :exhaust @16:19:11.47
CBV.dial SSV @16:19:12.82 CTV.dial :air,avoiding: :intake @16:19:14.16 CSV.dial CTV @16:19:14.19
SS.seek 10ml @16:19:15.41 CSV.dial :air @16:19:15.85 CS.reconfigure :fast @16:19:15.90 CS.seek 0ml @16:19:16.66
SSV.dial :spit,avoiding: :exhaust @16:19:18.01 SS.reconfigure :exhale @16:19:18.06 SS.seek 0ml @16:19:23.03 SS.reconfigure :fast @16:19:23.07 SS.seek 5ml @16:19:23.76 CS.stop @16:19:25.87 SSV.dial CBV,avoiding: :exhaust @16:19:25.89
CBV.dial SSV @16:19:28.25 SSV.dial :spit,avoiding: :exhaust @16:19:29.57 SS.reconfigure :default @16:19:29.62 SS.seek 0ml @16:19:32.81 SS.stop @16:19:32.86 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) Intake.select :close @16:19:32.93 CSV.dial :waste @16:19:33.06 SSV.dial CBV,avoiding: :exhaust @16:19:33.08
CBV.dial SSV @16:19:33.71 CS.seek 0ml @16:19:33.79 CSV.dial :air @16:19:34.44
CTV.dial :air @16:19:34.52 delay 15 seconds @16:19:34.56 CS.seek 10ml @16:19:49.54
SSV.dial :spit,avoiding: :exhaust @16:19:50.87 CTV.dial CSV,avoiding: :intake @16:19:50.90
SS.reconfigure :exhale @16:19:50.99 SS.seek 0ml @16:19:51.36 SSV.dial CBV,avoiding: :exhaust @16:19:59.05 SSV.dial between CBV and plug @16:19:59.54 CBV.dial between SSV and waste2 @16:19:59.93 CSV.dial between air and CTV @16:20:00.26 CS.seek 7.679ml @16:20:06.36 CTV.dial CSV,avoiding: :air @16:20:06.38
CSV.dial CTV @16:20:06.72 CS.reconfigure :pressurize @16:20:06.77 CS.seek 0ml @16:20:20.43 BubblePoint: Intake @24.2psia, Outlet @3.4psia @16:20:20.44 CBV.dial SSV @16:20:20.79 SS.reconfigure :default @16:20:20.83 SS.seek 10ml @16:20:26.83 delay 15 seconds @16:20:41.96 Evacuated: Intake @23.9psia, Outlet @23.9psia @16:20:41.97 SSV.dial :spit,avoiding: :exhaust @16:20:43.01 SS.reconfigure :exhale @16:20:43.06 SS.seek 0ml @16:20:48.06 SS.reconfigure :fast @16:20:48.11 SS.seek 5ml @16:20:51.07 SSV.dial CBV,avoiding: :exhaust @16:20:51.09
CBV.dial SSV @16:20:52.44 CTV.dial :air,avoiding: :intake @16:20:53.78 CSV.dial CTV @16:20:53.81
SS.seek 10ml @16:20:55.02 CSV.dial :air @16:20:55.45 CS.reconfigure :fast @16:20:55.50 CS.seek 0ml @16:20:56.55
SSV.dial :spit,avoiding: :exhaust @16:20:57.88 SS.reconfigure :exhale @16:20:57.93 SS.seek 0ml @16:21:02.17 CS.stop @16:21:03.03
SS.reconfigure :fast @16:21:03.07 SS.seek 5ml @16:21:05.90 SSV.dial CBV,avoiding: :exhaust @16:21:05.92
CBV.dial SSV @16:21:08.28 SSV.dial :spit,avoiding: :exhaust @16:21:09.61 SS.reconfigure :default @16:21:09.66 SS.seek 0ml @16:21:12.89 SS.stop @16:21:12.93 Lysing: Adding 2.0ml AqMeOH @16:21:12.94 CBV.dial between CSV and waste1,avoiding: :DBV @16:21:14.43 CTV.dial CSV @16:21:15.75 CSV.dial CSR @16:21:16.54 Pulling 1ml of CSR.AqMeOH CSR.select :AqMeOH @16:21:16.65 CS.reconfigure :default @16:21:16.70 CS.seek 1ml @16:21:20.11 delay 5 seconds @16:21:25.12 CSR.select :air @16:21:25.29 CS.seek 6ml @16:21:38.92 delay 2 seconds @16:21:40.93 CSV.dial :waste2 @16:21:41.38 CS.reconfigure :fast @16:21:41.42 CS.seek 0ml @16:21:55.70 CSV.dial CSR @16:21:56.15 Pulling 2ml of CSR.AqMeOH @16:21:56.16 CSR.select :AqMeOH @16:21:56.28 CS.reconfigure :default @16:21:56.32 CS.seek 2ml @16:22:02.23 delay 5 seconds @16:22:07.25 CSR.select :air @16:22:07.84 CS.seek 4.999ml @16:22:16.35 delay 2 seconds @16:22:18.37 CSV.dial CTV @16:22:18.83 CS.seek 1ml @16:22:30.21 Before lysing Collection Puck Heater at 13.1C/29.9psia @16:22:30.23 Heating Puck.from 4, :sh1 in Collection Clamp to 70C for 10:00 @16:22:30.25 CH.seek 70C,8:00 @16:24:42.88 CH.hold 10:00,70C @16:24:43.08 Begun lysing Collection Puck Heater at 68.1C/37.5psia @16:34:43.10 Ended lysing Collection Puck Heater at 70C/36.3psia @16:34:43.11 CS.seek 0ml @16:34:46.57 CTV.dial between spare and air,avoiding: :intake @16:34:47.59 CSV.dial :air @16:34:48.04 CS.seek 5ml @16:35:01.84 CSV.dial CTV @16:35:02.30 delay 2 seconds @16:35:04.31 CSV.dial :air @16:35:04.76 delay 2 seconds @16:35:06.78 CSV.dial CTV @16:35:07.24 delay 2 seconds @16:35:09.25 CSV.dial :air @16:35:09.70 delay 2 seconds @16:35:11.72 CS.seek 0ml @16:35:25.34 Puck @79.1psia after final lysis pressurization @16:35:25.35 Recovering Lysate into Collection syringe @16:35:25.36 CSV.dial CBV @16:35:26.37 CBV.dial CSV @16:35:26.76 CS.seek 5ml @16:35:40.43 delay 5 seconds @16:35:45.45 CTV.dial :air @16:35:45.98 CS.seek 7ml @16:35:51.90 delay 5 seconds @16:35:56.92 CSV.dial :air2 @16:35:57.36 CS.seek 10ml @16:36:05.84 CSV.dial between air and CTV,avoiding: CBV @16:36:07.00 Lysing Complete @16:36:07.07 Storing sh1 puck in Collection Clamp to tube 3 @16:36:07.24 SC.reconfigure :tight @16:36:07.29 SC.seek 3 @16:36:10.98 SC.configure :limp @16:36:11.20 Elbow.reconfigure :tight @16:36:11.25 Elbow.seek 3 @16:36:12.96 Forearm.reconfigure :tight @16:36:13.01 Forearm.seek 3 + 450 counts @16:36:14.18 SE.reconfigure :pick @16:36:14.24 SE.seek :beamBreak @16:36:16.17 SE.configure :default @16:36:16.34 Elbow.reconfigure :limp @16:36:16.39 Forearm.reconfigure :loose @16:36:16.43 Forearm.seek 3 @16:36:16.73 Elbow.hold @16:36:16.79 Elbow.reconfigure :tight @16:36:16.89 SE.seek 17.12 Pucks below Top Plate @16:36:17.36 Forearm.force -20 @16:36:17.40 Forearm.seek 3 + 296 counts @16:36:17.77 Forearm.reconfigure :tight @16:36:17.82 Forearm.seek 3 + 450 counts @16:36:18.03 SE.seek 18.12 Pucks below Top Plate @16:36:18.59 Forearm.reconfigure :loose @16:36:18.64 Forearm.seek :retract @16:36:19.59 Elbow.seek CC @16:36:21.28 Elbow.reconfigure :loose @16:36:21.38 Forearm.seek CC @16:36:22.46 Hand.close @16:36:23.06 CC.seek :open @16:36:27.79 Forearm.seek :retract @16:36:28.78 Elbow.reconfigure :tight @16:36:28.82 Elbow.seek 3 @16:36:30.66 Elbow.reconfigure :limp @16:36:30.71 Forearm.seek 3 @16:36:31.68 Elbow.hold @16:36:31.75 Elbow.reconfigure :tight @16:36:31.80 Hand.open @16:36:32.55 Forearm.force -20 @16:36:32.60 Forearm.seek 3 + 319 counts @16:36:32.91 Forearm.reconfigure :tight @16:36:32.96 Forearm.seek 3 + 450 counts @16:36:33.22 SE.seek :down @16:36:34.35
Forearm.reconfigure :loose @16:36:34.40 Forearm.seek :retract @16:36:34.67 SE.stop @16:36:35.39
Tranferring DA @16:36:35.40 Beginning samplePassC @16:36:35.41 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @16:36:35.60 Elbow.seek FlushPuck::Garage @16:36:37.35 Elbow.reconfigure :loose @16:36:37.45 Forearm.seek :garage @16:36:38.40 Hand.close @16:36:39.17 Forearm.seek :retract @16:36:40.12 Elbow.reconfigure :tight @16:36:40.17 Elbow.seek CC @16:36:43.14 Elbow.reconfigure :loose @16:36:43.24 Forearm.reconfigure :tight @16:36:43.30 Forearm.seek CC @16:36:44.57 CC.seek :closed @16:36:49.57 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.32 @16:36:49.64 Hand.open @16:36:50.37 Forearm.reconfigure :loose @16:36:50.42 Forearm.seek :retract @16:36:51.43 PSV.dial PSR @16:36:52.85 Pulling 1.5ml of PSR.flush @16:36:52.86 PSR.select :flush @16:36:52.98 PS.seek 1.5ml @16:37:09.16 delay 5 seconds @16:37:14.18 PSV.dial :waste1 @16:37:14.66 PS.seek 0ml @16:37:30.75 PSV.dial PSR @16:37:31.23 PSR.select :air @16:37:31.35 PS.seek 2.5ml @16:37:57.66 PRV.dial CBV,avoiding: :ab2 @16:37:57.68
CBV.dial PRV @16:37:59.44 PSV.dial :mixloop @16:38:00.49 CTV.dial :air,avoiding: :intake @16:38:00.70 PTV.dial :PRVmixing @16:38:02.02 PS.seek 0ml @16:38:28.37 PSV.dial :air @16:38:28.85 PS.seek 2.5ml @16:38:55.25 PSV.dial :mixloop @16:38:55.74 PS.seek 0ml @16:39:22.09 PSV.dial :air @16:39:22.69 PS.seek 2.5ml @16:39:49.06 PSV.dial :mixloop @16:39:49.65 PS.seek 0ml @16:40:15.97 Garaging FlushPuck in Collection Clamp @16:40:16.04 Elbow.reconfigure :tight @16:40:16.09 Elbow.seek CC @16:40:16.39 Elbow.reconfigure :loose @16:40:16.87 Forearm.seek CC @16:40:17.94 Hand.close @16:40:18.56 CC.seek :open @16:40:23.20 Forearm.seek :retract @16:40:24.21 Elbow.reconfigure :tight @16:40:24.26 Elbow.seek :garage @16:40:27.13 Elbow.reconfigure :loose @16:40:27.23 Forearm.reconfigure :tight @16:40:27.27 Forearm.seek :garage @16:40:28.33 Hand.open @16:40:28.99 Forearm.reconfigure :loose @16:40:29.04 Forearm.seek :retract @16:40:30.02 PSV.dial :mixloop @16:40:30.03 PTV.dial :PRVmixing @16:40:30.11 PRV.dial CBV @16:40:30.13
CBV.dial PRV @16:40:30.34 CTV.dial :air,avoiding: :intake @16:40:30.36 Loading lyfil puck from tube 4 into Collection Clamp @16:40:30.69 SC.reconfigure :tight @16:40:30.73 SC.seek 4 @16:40:34.31 SC.configure :limp @16:40:34.53 Elbow.reconfigure :tight @16:40:34.58 Elbow.seek 4 @16:40:36.50 Forearm.reconfigure :tight @16:40:36.54 Forearm.seek 4 + 450 counts @16:40:37.60 SE.reconfigure :pick @16:40:37.66 SE.seek :beamBreak @16:40:39.92 SE.configure :default @16:40:40.08 Elbow.seek 4 @16:40:40.68 Forearm.seek 4 + 300 counts @16:40:40.97 Forearm.reconfigure :loose @16:40:41.02 Hand.close @16:40:41.70 SE.seek :down @16:40:41.90
Forearm.seek :retract @16:40:42.85 Elbow.seek CC @16:40:43.58 SE.stop @16:40:44.50
Elbow.reconfigure :loose @16:40:44.60 Forearm.reconfigure :tight @16:40:44.65 Forearm.seek CC @16:40:45.95 CC.seek :closed @16:40:50.47 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.14 @16:40:50.48 Hand.open @16:40:51.11 Forearm.reconfigure :loose @16:40:51.16 Forearm.seek :retract @16:40:52.16 CTV.dial CSV,avoiding: :intake @16:40:53.49 CSV.dial CTV @16:40:53.83 PS.seek 2.5ml @16:41:20.27 CS.seek 4.5ml @16:41:35.21 delay 8 seconds @16:41:43.23 PSV.dial :air @16:41:43.81 delay 2 seconds @16:41:45.83 PSV.dial :waste2 @16:41:46.60 PS.seek 0ml @16:42:12.87 CBV.dial between PRV and waste1 @16:42:13.19 CSV.dial :air @16:42:13.67 CS.seek 10ml @16:42:28.61 CSV.dial CTV @16:42:29.52 CS.seek 0ml @16:42:56.11 CBV.dial :waste1 @16:42:56.43 delay 5 seconds @16:43:01.45 CTV.dial :air,avoiding: :intake @16:43:02.86 Storing lyfil puck in Collection Clamp to tube 3 @16:43:03.04 SC.reconfigure :tight @16:43:03.09 SC.seek 3 @16:43:06.66 SC.configure :limp @16:43:06.88 Elbow.reconfigure :tight @16:43:06.92 Elbow.seek 3 @16:43:08.70 Forearm.reconfigure :tight @16:43:08.75 Forearm.seek 3 + 450 counts @16:43:09.83 SE.reconfigure :pick @16:43:09.89 SE.seek :beamBreak @16:43:11.90 SE.configure :default @16:43:12.06 Elbow.reconfigure :limp @16:43:12.11 Forearm.reconfigure :loose @16:43:12.15 Forearm.seek 3 @16:43:12.41 Elbow.hold @16:43:12.48 Elbow.reconfigure :tight @16:43:12.58 SE.seek 18.12 Pucks below Top Plate @16:43:13.03 Forearm.force -20 @16:43:13.07 Forearm.seek 3 + 312 counts @16:43:14.18 Forearm.reconfigure :tight @16:43:14.23 Forearm.seek 3 + 450 counts @16:43:14.43 SE.seek 19.12 Pucks below Top Plate @16:43:15.07 Forearm.reconfigure :loose @16:43:15.12 Forearm.seek :retract @16:43:16.05 Elbow.seek CC @16:43:18.17 Elbow.reconfigure :loose @16:43:18.27 Forearm.seek CC @16:43:19.30 Hand.close @16:43:19.93 CC.seek :open @16:43:24.66 Forearm.seek :retract @16:43:25.65 Elbow.reconfigure :tight @16:43:25.69 Elbow.seek 3 @16:43:27.48 Elbow.reconfigure :limp @16:43:27.53 Forearm.seek 3 @16:43:28.58 Elbow.hold @16:43:28.65 Elbow.reconfigure :tight @16:43:28.69 Hand.open @16:43:29.42 Forearm.force -20 @16:43:29.47 Forearm.seek 3 + 308 counts @16:43:29.84 Forearm.reconfigure :tight @16:43:29.89 Forearm.seek 3 + 450 counts @16:43:30.23 SE.seek :down @16:43:31.36
Forearm.reconfigure :loose @16:43:31.41 Forearm.seek :retract @16:43:31.65 SE.stop @16:43:32.36
PSV.dial :air @16:43:33.11 PS.seek 2.5ml @16:43:59.46 delay 1 second @16:44:00.48 PSV.dial :mixloop @16:44:00.93 delay 1 second @16:44:01.95 PSV.dial :air @16:44:02.42 delay 1 second @16:44:03.43 PSV.dial :mixloop @16:44:03.90 delay 1 second @16:44:04.92 PSV.dial :air @16:44:05.39 delay 1 second @16:44:06.41 PS.seek 0ml @16:44:33.18 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @16:44:33.37 Elbow.seek FlushPuck::Garage @16:44:35.12 Elbow.reconfigure :loose @16:44:35.22 Forearm.seek :garage @16:44:36.24 Hand.close @16:44:36.92 Forearm.seek :retract @16:44:37.94 Elbow.reconfigure :tight @16:44:37.99 Elbow.seek PC @16:44:39.26 Elbow.reconfigure :loose @16:44:39.36 Forearm.reconfigure :tight @16:44:39.41 Forearm.seek PC @16:44:40.65 PC.seek :closed @16:44:45.29 Processing Clamp onSpring/offSpring for 16/26 samples, on/off torque 1.66 @16:44:45.37 Hand.open @16:44:45.98 Forearm.reconfigure :loose @16:44:46.03 Forearm.seek :retract @16:44:46.99 PRV.dial :air @16:44:47.43 PSV.dial :mixloop @16:44:47.96 PS.seek 0.3ml @16:44:51.84 PSV.dial PSR @16:44:52.90 Pulling 0.4ml of PSR.air @16:44:52.91 PSR.select :air @16:44:52.93 PS.seek 0.7ml @16:44:57.81 delay 3 seconds @16:45:00.82 PSR.select :air @16:45:00.85 PS.seek 2.5ml @16:45:20.45 delay 3 seconds @16:45:23.49 PSV.dial :mixloop @16:45:24.54 PTV.dial :mixing @16:45:25.30 PBV.dial :waste2 @16:45:26.26 PS.seek 0ml @16:45:52.62 Garaging FlushPuck in Processing Clamp @16:45:52.69 Elbow.reconfigure :tight @16:45:52.74 Elbow.seek PC @16:45:52.86 Elbow.reconfigure :loose @16:45:52.96 Forearm.seek PC @16:45:53.94 Hand.close @16:45:54.55 PC.seek :open @16:45:59.27 Forearm.seek :retract @16:46:00.22 Elbow.reconfigure :tight @16:46:00.27 Elbow.seek :garage @16:46:01.65 Elbow.reconfigure :loose @16:46:01.75 Forearm.reconfigure :tight @16:46:01.80 Forearm.seek :garage @16:46:02.84 Hand.open @16:46:03.48 Forearm.reconfigure :loose @16:46:03.53 Forearm.seek :retract @16:46:04.53 Loading da puck from tube 4 into Processing Clamp @16:46:04.77 SC.reconfigure :tight @16:46:04.81 SC.seek 4 @16:46:08.37 SC.configure :limp @16:46:08.58 Elbow.reconfigure :tight @16:46:08.63 Elbow.seek 4 @16:46:10.49 Forearm.reconfigure :tight @16:46:10.54 Forearm.seek 4 + 450 counts @16:46:11.67 SE.reconfigure :pick @16:46:11.73 SE.seek :beamBreak @16:46:14.05 SE.configure :default @16:46:14.64 Elbow.seek 4 @16:46:14.83 Forearm.seek 4 + 300 counts @16:46:15.06 Forearm.reconfigure :loose @16:46:15.11 Hand.close @16:46:15.70 SE.seek :down @16:46:15.91
Forearm.seek :retract @16:46:16.86 Elbow.seek PC @16:46:17.73 SE.stop @16:46:19.43
Elbow.reconfigure :loose @16:46:19.53 Forearm.reconfigure :tight @16:46:19.58 Forearm.seek PC @16:46:20.89 PC.seek :closed @16:46:25.55 Processing Clamp onSpring/offSpring for 16/26 samples, on/off torque 1.62 @16:46:25.57 Hand.open @16:46:26.23 Forearm.reconfigure :loose @16:46:26.28 Forearm.seek :retract @16:46:27.23 Heating Puck.from 4, :da in Processing Clamp to 28C for 2:00:00 @16:46:27.25 PH.seek 28C,5:00 @16:47:09.92 PH.hold 2:00:00,28C @16:47:09.97 delay 1:30 @16:48:40.02 PSR.select :air @16:48:40.03 PSV.dial PSR @16:48:41.10 Pulling 0.5ml of PSR.wash @16:48:41.11 PSR.select :wash @16:48:41.23 PS.seek 0.5ml @16:48:47.11 delay 5 seconds @16:48:52.13 PSR.select :air @16:48:52.25 PS.seek 2ml @16:49:08.33 PTV.dial :puck @16:49:09.08 PSV.dial PTV @16:49:09.85 PBV.dial :waste1 @16:49:11.24 PS.seek 1.5ml @16:49:17.49 delay 3 seconds @16:49:20.51 PBV.dial between PSV and waste1 @16:49:20.87 PS.seek 0ml @16:49:37.05 delay 5:00 @16:54:37.07 recoverPSRreagent08 @16:54:37.08 PTV.dial :puck @16:54:37.15 PBV.dial :waste1 @16:54:37.53 delay 30 seconds @16:55:07.56 PBV.dial between PSV and waste1 @16:55:07.93 PSR.select :air @16:55:07.95 PSV.dial PSR @16:55:08.71 PS.reconfigure :fast @16:55:08.75 PS.seek 2.5ml @16:55:32.54 PSV.dial PTV @16:55:33.31 delay 5 seconds @16:55:38.33 PSV.dial PSR @16:55:39.53 delay 5 seconds @16:55:44.55 PSV.dial PTV @16:55:45.31 delay 5 seconds @16:55:50.33 PSV.dial :air @16:55:51.10 PS.seek 0ml @16:56:14.30 PTV.dial :PRVmixing,via: PRV @16:56:15.62 PSV.dial :mixloop @16:56:16.11 PRV.dial :ab1 @16:56:16.86 PS.reconfigure :slow @16:56:16.91 PS.seek 0.15ml @16:56:20.45 delay 2 seconds @16:56:22.47 CBV.dial PRV @16:56:22.49
PRV.dial CBV @16:56:23.08 PS.seek 0.35ml @16:56:27.61 delay 2 seconds @16:56:29.62 CBV.dial between PRV and PSV @16:56:29.95 PRV.dial :air @16:56:30.41 PS.seek 0.9ml @16:56:42.20 PRV.dial :waste2 @16:56:42.94 PS.seek 0.5ml @16:56:51.57 addPMLReagent08 @16:56:51.58 PSV.dial :air @16:56:52.17 PS.reconfigure :default @16:56:52.22 PS.seek 2ml @16:57:08.42 PSV.dial :PML @16:57:08.87 PTV.dial :mixing @16:57:09.62 PBV.dial :waste1 @16:57:10.06 PS.seek 1.85ml @16:57:12.39 PBV.dial between PSV and waste1 @16:57:12.77 PS.seek 0ml @16:57:32.51 delay 2 seconds @16:57:34.53 Beginning samplePassC @16:57:34.54 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @16:57:34.73 Elbow.reconfigure :tight @16:57:34.77 Elbow.seek FlushPuck::Garage @16:57:36.14 Elbow.reconfigure :loose @16:57:36.24 Forearm.seek :garage @16:57:37.23 Hand.close @16:57:37.92 Forearm.seek :retract @16:57:38.86 Elbow.reconfigure :tight @16:57:39.33 Elbow.seek CC @16:57:42.19 Elbow.reconfigure :loose @16:57:42.29 Forearm.reconfigure :tight @16:57:42.34 Forearm.seek CC @16:57:43.74 CC.seek :closed @16:57:48.27 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.44 @16:57:48.33 Hand.open @16:57:48.98 Forearm.reconfigure :loose @16:57:49.02 Forearm.seek :retract @16:57:49.99 PSV.dial PSR @16:57:51.18 Pulling 1.5ml of PSR.flush @16:57:51.19 PSR.select :flush @16:57:51.31 PS.seek 1.5ml @16:58:07.51 delay 5 seconds @16:58:12.53 PSV.dial :waste1 @16:58:13.00 PS.seek 0ml @16:58:29.10 PSV.dial PSR @16:58:29.57 PSR.select :air @16:58:29.69 PS.seek 2.5ml @16:58:56.02 PRV.dial CBV,avoiding: :ab2 @16:58:56.04
CBV.dial PRV @16:58:57.68 PSV.dial :mixloop @16:58:58.73 CTV.dial :air,avoiding: :intake @16:58:58.96 PTV.dial :PRVmixing @16:58:59.73 PS.seek 0ml @16:59:26.26 PSV.dial :air @16:59:26.73 PS.seek 2.5ml @16:59:53.13 PSV.dial :mixloop @16:59:53.61 PS.seek 0ml @17:00:19.98 PSV.dial :air @17:00:20.52 PS.seek 2.5ml @17:00:46.94 PSV.dial :mixloop @17:00:47.42 PS.seek 0ml @17:01:13.70 Garaging FlushPuck in Collection Clamp @17:01:13.77 Elbow.reconfigure :tight @17:01:13.81 Elbow.seek CC @17:01:14.09 Elbow.reconfigure :loose @17:01:14.20 Forearm.seek CC @17:01:15.16 Hand.close @17:01:15.79 CC.seek :open @17:01:20.43 Forearm.seek :retract @17:01:21.45 Elbow.reconfigure :tight @17:01:21.49 Elbow.seek :garage @17:01:24.42 Elbow.reconfigure :loose @17:01:24.52 Forearm.reconfigure :tight @17:01:24.57 Forearm.seek :garage @17:01:25.56 Hand.open @17:01:26.23 Forearm.reconfigure :loose @17:01:26.27 Forearm.seek :retract @17:01:27.27 delayUntil "17:05:34.52" @17:01:27.30 Whole Cell Archive sampling 1000ml @17:01:27.32 Loading wcr puck from tube 4 into Collection Clamp @17:01:27.80 Elbow.reconfigure :tight @17:01:27.85 Elbow.seek 4 @17:01:29.80 Forearm.reconfigure :tight @17:01:29.85 Forearm.seek 4 + 450 counts @17:01:30.89 SE.reconfigure :pick @17:01:30.95 SE.seek :beamBreak @17:01:33.39 SE.configure :default @17:01:34.00 Elbow.seek 4 @17:01:34.20 Forearm.seek 4 + 300 counts @17:01:34.44 Forearm.reconfigure :loose @17:01:34.49 Hand.close @17:01:35.18 SE.seek :down @17:01:35.38 Forearm.seek :retract @17:01:36.34 Elbow.seek CC @17:01:37.23 SE.stop @17:01:37.95 Elbow.reconfigure :loose @17:01:38.06 Forearm.reconfigure :tight @17:01:38.11 Forearm.seek CC @17:01:39.36 CC.seek :closed @17:01:43.95 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.35 @17:01:43.96 Hand.open @17:01:44.60 Forearm.reconfigure :loose @17:01:44.65 Forearm.seek :retract @17:01:45.66 collectSample 1000ml w/bubble point of 35psi @17:01:45.68 Priming 7ml @17:01:45.69 Intake.select :close @17:01:45.70 Exhaust.select :close @17:01:45.71 SS.hold @17:01:45.92 Calibrating pressure sensors @17:01:45.93 CTV.dial :air @17:01:46.01 SSV.dial :spit,avoiding: :exhaust @17:01:46.10 SS.reconfigure :fast @17:01:46.61 SS.seek 5ml @17:01:49.33 SSV.dial CBV @17:01:49.36 CBV.dial SSV @17:01:50.70 delay 3.8 seconds @17:01:54.64 Sensor limits: Intake max 104.6psia; Outlet max 96.0psia @17:01:54.65 SSV.dial :spit @17:01:55.98 SS.seek 0ml @17:01:58.84 SSV.dial CBV @17:02:00.17 CTV.dial :intake @17:02:00.98 SSV.dial CBV @17:02:01.00 CBV.dial SSV @17:02:01.08 Allowing 40s for 7.0ml of remaining 7.0ml @17:02:01.10 Intake.select :open @17:02:03.26 Intake @14.6psia, vacuum from 9.1 to 12.1psi @17:02:03.28 SS.reconfigure :inhaling 2.20ml/s @17:02:03.33 SS.seek 7ml @17:02:10.28 Stroke completed -- waiting for pressure to equalize ... @17:02:10.29 SS.reconfigure :exhale @17:02:10.34 SS.hold @17:02:29.16 Intake.select :close @17:02:29.33 SSV.dial :exhaust @17:02:30.11 Exhaust.select :open @17:02:30.23 SS.seek 0ml @17:02:34.36 Exhaust.select :close @17:02:34.47 Sampling 1000.0ml @17:02:34.48 Intake.select :close @17:02:34.50 Exhaust.select :close @17:02:34.51 SS.reconfigure :default @17:02:34.56 SS.hold @17:02:34.77 CTV.dial :intake @17:02:34.94 SSV.dial CBV @17:02:34.99 CBV.dial SSV @17:02:35.73 Allowing 130s for 25.0ml of remaining 1000.0ml @17:02:35.75 Intake.select :open @17:02:37.92 Intake @15.4psia, vacuum from 9.9 to 12.9psi @17:02:37.93 SS.reconfigure :inhaling 2.20ml/s @17:02:37.98 SS.seek 25ml @17:02:46.02 Vacuum too high -- waiting up to 40 seconds @17:02:46.04 SS.reconfigure :exhale @17:02:46.09 SS.hold @17:02:55.05 Intake @18.9psia, vacuum from 10.0 to 13.0psi @17:02:55.07 SS.reconfigure :inhaling 1.76ml/s @17:02:55.12 SS.seek 25ml @17:02:57.27 Vacuum too high -- waiting up to 40 seconds @17:02:57.29 SS.reconfigure :exhale @17:02:57.34 SS.hold @17:02:59.62 Intake @18.8psia, vacuum from 10.0 to 13.0psi @17:02:59.63 SS.reconfigure :inhaling 1.41ml/s @17:02:59.68 SS.seek 25ml @17:03:01.93 Vacuum too high -- waiting up to 40 seconds @17:03:01.94 SS.reconfigure :exhale @17:03:02.00 SS.hold @17:03:04.16 Intake @18.9psia, vacuum from 10.0 to 13.0psi @17:03:04.18 SS.reconfigure :inhaling 1.13ml/s @17:03:04.23 SS.seek 25ml @17:03:06.74 Vacuum too high -- waiting up to 40 seconds @17:03:06.76 SS.reconfigure :exhale @17:03:06.81 SS.hold @17:03:08.84 Intake @19.1psia, vacuum from 10.0 to 13.0psi @17:03:08.86 SS.reconfigure :inhaling 0.91ml/s @17:03:08.90 SS.seek 25ml @17:03:12.39 Vacuum too high -- waiting up to 40 seconds @17:03:12.41 SS.reconfigure :exhale @17:03:12.46 SS.hold @17:03:14.60 SS.reconfigure :inhaling 0.73ml/s @17:03:14.65 SS.seek 25ml @17:03:20.93 Stroke completed -- waiting for pressure to equalize ... @17:03:20.94 SS.reconfigure :exhale @17:03:20.99 SS.hold @17:03:49.63 Intake.select :close @17:03:49.79 SSV.dial :exhaust @17:03:50.56 Exhaust.select :open @17:03:50.68 SS.seek 0ml @17:03:59.71 Exhaust.select :close @17:03:59.82 SSV.dial CBV @17:03:59.85 CBV.dial SSV @17:04:00.62 Allowing 130s for 25.0ml of remaining 975.0ml @17:04:00.64 Intake.select :open @17:04:02.80 Intake @15.9psia, vacuum from 10.0 to 13.0psi @17:04:02.82 SS.reconfigure :inhaling 0.73ml/s @17:04:02.87 SS.seek 25ml @17:04:39.21 Stroke completed -- waiting for pressure to equalize ... @17:04:39.22 SS.reconfigure :exhale @17:04:39.27 SS.hold @17:05:12.19 Intake.select :close @17:05:12.36 SSV.dial :exhaust @17:05:13.14 Exhaust.select :open @17:05:13.26 SS.seek 0ml @17:05:22.20 Exhaust.select :close @17:05:22.31 SSV.dial CBV @17:05:22.34 CBV.dial SSV @17:05:23.14 Allowing 130s for 25.0ml of remaining 950.0ml @17:05:23.15 Intake.select :open @17:05:25.33 Intake @16.0psia, vacuum from 10.0 to 13.0psi @17:05:25.35 SS.reconfigure :inhaling 0.73ml/s @17:05:25.39 SS.seek 25ml @17:05:34.53 recoverPRVreagent08 @17:05:34.54 PTV.dial :mixing @17:05:35.31 PBV.dial :waste1 @17:05:35.69 delay 30 seconds @17:06:01.68 Stroke completed -- waiting for pressure to equalize ... @17:06:01.69 SS.reconfigure :exhale @17:06:01.74 SS.hold @17:06:05.71 PBV.dial between PSV and waste1 @17:06:06.08 PSV.dial :air @17:06:06.61 PS.reconfigure :fast @17:06:06.72 PS.seek 2.5ml @17:06:30.87 PSV.dial :mixloop @17:06:31.35 delay 2 seconds @17:06:32.41 Intake.select :close @17:06:32.57 SSV.dial :exhaust @17:06:33.34 Exhaust.select :open @17:06:33.40 PSV.dial :air @17:06:33.46 SS.seek 0ml @17:06:33.86 delay 2 seconds @17:06:35.88 PSV.dial :mixloop @17:06:36.35 delay 2 seconds @17:06:38.36 PSV.dial :air @17:06:38.83 PS.seek 0ml @17:06:42.31 Exhaust.select :close @17:06:42.41 SSV.dial CBV @17:06:42.45 CBV.dial SSV @17:06:43.21 Allowing 130s for 25.0ml of remaining 925.0ml @17:06:43.23 Intake.select :open @17:06:45.40 Intake @16.0psia, vacuum from 10.0 to 13.0psi @17:06:45.42 SS.reconfigure :inhaling 0.73ml/s @17:06:45.47 SS.seek 25ml @17:07:02.69 washCycleDA #1 of 2 @17:07:02.70 PSR.select :air @17:07:02.72 PSV.dial PSR @17:07:04.06 Pulling 0.5ml of PSR.wash @17:07:04.07 PSR.select :wash @17:07:04.19 PS.reconfigure :default @17:07:04.24 PS.seek 0.5ml @17:07:10.25 delay 5 seconds @17:07:15.27 PSR.select :air @17:07:15.39 PS.seek 2ml @17:07:22.13 Stroke completed -- waiting for pressure to equalize ... @17:07:22.14 SS.reconfigure :exhale @17:07:22.19 SS.hold @17:07:31.56 PTV.dial :puck @17:07:32.31 PSV.dial PTV @17:07:33.06 PBV.dial :waste1 @17:07:33.50 PS.seek 1.5ml @17:07:39.44 delay 3 seconds @17:07:42.45 PBV.dial between PSV and waste1 @17:07:42.83 PS.seek 0ml @17:07:53.80 Intake.select :close @17:07:53.96 SSV.dial :exhaust @17:07:54.73 Exhaust.select :open @17:07:54.85 SS.seek 0ml @17:07:58.97 delay 2:00 @17:08:03.62 Exhaust.select :close @17:08:03.73 SSV.dial CBV @17:08:03.75 CBV.dial SSV @17:08:04.54 Allowing 130s for 25.0ml of remaining 900.0ml @17:08:04.55 Intake.select :open @17:08:06.77 Intake @16.0psia, vacuum from 10.0 to 13.0psi @17:08:06.79 SS.reconfigure :inhaling 0.73ml/s @17:08:06.84 SS.seek 25ml @17:08:43.38 Stroke completed -- waiting for pressure to equalize ... @17:08:43.39 SS.reconfigure :exhale @17:08:43.44 SS.hold @17:09:10.01 Intake.select :close @17:09:10.17 SSV.dial :exhaust @17:09:10.93 Exhaust.select :open @17:09:11.05 SS.seek 0ml @17:09:20.10 Exhaust.select :close @17:09:20.21 SSV.dial CBV @17:09:20.25 CBV.dial SSV @17:09:21.01 Allowing 130s for 25.0ml of remaining 875.0ml @17:09:21.02 Intake.select :open @17:09:23.19 Intake @16.0psia, vacuum from 10.0 to 13.0psi @17:09:23.21 SS.reconfigure :inhaling 0.73ml/s @17:09:23.26 SS.seek 25ml @17:09:58.61 Vacuum too high -- waiting up to 40 seconds @17:09:58.62 SS.reconfigure :exhale @17:09:58.67 SS.hold @17:09:59.06 recoverPSRreagent08 @17:09:59.07 PTV.dial :puck @17:09:59.14 PBV.dial :waste1 @17:09:59.51 delay 30 seconds @17:10:06.38 Intake @17.8psia, vacuum from 10.0 to 13.0psi @17:10:06.39 SS.reconfigure :inhaling 0.59ml/s @17:10:06.44 SS.seek 25ml @17:10:07.17 Stroke completed -- waiting for pressure to equalize ... @17:10:07.19 SS.reconfigure :exhale @17:10:07.23 SS.hold @17:10:23.56 Intake.select :close @17:10:24.18 SSV.dial :exhaust @17:10:24.94 Exhaust.select :open @17:10:25.06 SS.seek 0ml @17:10:29.53 PBV.dial between PSV and waste1 @17:10:29.90 PSR.select :air @17:10:29.92 PSV.dial PSR @17:10:30.71 PS.reconfigure :fast @17:10:30.76 PS.seek 2.5ml @17:10:33.88 Exhaust.select :close @17:10:33.99 SSV.dial CBV @17:10:34.02 CBV.dial SSV @17:10:34.82 Allowing 130s for 25.0ml of remaining 850.0ml @17:10:34.83 Intake.select :open @17:10:37.00 Intake @16.0psia, vacuum from 10.0 to 13.0psi @17:10:37.02 SS.reconfigure :inhaling 0.59ml/s @17:10:37.07 SS.seek 25ml @17:10:54.87 PSV.dial PTV @17:10:55.65 delay 5 seconds @17:11:00.67 PSV.dial PSR @17:11:01.45 delay 5 seconds @17:11:06.47 PSV.dial PTV @17:11:07.23 delay 5 seconds @17:11:12.25 PSV.dial :air @17:11:13.03 PS.seek 0ml @17:11:21.91 Stroke completed -- waiting for pressure to equalize ... @17:11:21.92 SS.reconfigure :exhale @17:11:21.97 SS.hold @17:11:36.49 washCycleDA #2 of 2 @17:11:36.51 PSR.select :air @17:11:36.52 PSV.dial PSR @17:11:37.85 Pulling 0.5ml of PSR.wash @17:11:37.86 PSR.select :wash @17:11:37.98 PS.reconfigure :default @17:11:38.03 PS.seek 0.5ml @17:11:40.79 Intake.select :close @17:11:40.95 SSV.dial :exhaust @17:11:41.72 Exhaust.select :open @17:11:41.84 SS.seek 0ml @17:11:43.96 delay 5 seconds @17:11:48.98 PSR.select :air @17:11:49.11 PS.seek 2ml @17:11:50.88 Exhaust.select :close @17:11:50.99 SSV.dial CBV @17:11:51.02 CBV.dial SSV @17:11:51.80 Allowing 130s for 25.0ml of remaining 825.0ml @17:11:51.82 Intake.select :open @17:11:53.98 Intake @16.0psia, vacuum from 10.0 to 13.0psi @17:11:54.00 SS.reconfigure :inhaling 0.59ml/s @17:11:54.05 SS.seek 25ml @17:12:05.34 PTV.dial :puck @17:12:05.35 PSV.dial PTV @17:12:06.10 PBV.dial :waste1 @17:12:06.55 PS.seek 1.5ml @17:12:12.49 delay 3 seconds @17:12:15.51 PBV.dial between PSV and waste1 @17:12:15.89 PS.seek 0ml @17:12:32.00 delay 2:00 @17:12:38.34 Stroke completed -- waiting for pressure to equalize ... @17:12:38.36 SS.reconfigure :exhale @17:12:38.40 SS.hold @17:12:59.30 Intake.select :close @17:12:59.46 SSV.dial :exhaust @17:13:00.24 Exhaust.select :open @17:13:00.36 SS.seek 0ml @17:13:09.36 Exhaust.select :close @17:13:09.47 SSV.dial CBV @17:13:09.51 CBV.dial SSV @17:13:10.28 Allowing 130s for 25.0ml of remaining 800.0ml @17:13:10.30 Intake.select :open @17:13:12.46 Intake @15.9psia, vacuum from 10.0 to 13.0psi @17:13:12.48 SS.reconfigure :inhaling 0.59ml/s @17:13:12.53 SS.seek 25ml @17:13:56.94 Stroke completed -- waiting for pressure to equalize ... @17:13:56.95 SS.reconfigure :exhale @17:13:57.00 SS.hold @17:14:17.70 Intake.select :close @17:14:17.86 SSV.dial :exhaust @17:14:18.63 Exhaust.select :open @17:14:18.75 SS.seek 0ml @17:14:27.53 Exhaust.select :close @17:14:27.65 SSV.dial CBV @17:14:27.67 CBV.dial SSV @17:14:28.45 Allowing 130s for 25.0ml of remaining 775.0ml @17:14:28.47 Intake.select :open @17:14:31.05 Intake @16.0psia, vacuum from 10.0 to 13.0psi @17:14:31.06 SS.reconfigure :inhaling 0.59ml/s @17:14:31.11 SS.seek 25ml @17:14:32.02 recoverPSRreagent08 @17:14:32.03 PTV.dial :puck @17:14:32.04 PBV.dial :waste1 @17:14:32.42 delay 30 seconds @17:15:02.44 PBV.dial between PSV and waste1 @17:15:02.82 PSR.select :air @17:15:02.84 PSV.dial PSR @17:15:03.61 PS.reconfigure :fast @17:15:03.66 PS.seek 2.5ml @17:15:15.78 Stroke completed -- waiting for pressure to equalize ... @17:15:15.79 SS.reconfigure :exhale @17:15:15.84 SS.hold @17:15:27.80 PSV.dial PTV @17:15:28.59 delay 5 seconds @17:15:33.61 PSV.dial PSR @17:15:34.55 delay 5 seconds @17:15:36.30 Intake.select :close @17:15:36.46 SSV.dial :exhaust @17:15:37.24 Exhaust.select :open @17:15:37.36 SS.seek 0ml @17:15:39.57 PSV.dial PTV @17:15:40.32 delay 5 seconds @17:15:45.34 PSV.dial :air @17:15:46.11 Exhaust.select :close @17:15:46.23 PS.seek 0ml @17:15:46.28 SSV.dial CBV @17:15:46.33 CBV.dial SSV @17:15:47.10 Allowing 130s for 25.0ml of remaining 750.0ml @17:15:47.11 Intake.select :open @17:15:49.28 Intake @16.1psia, vacuum from 10.0 to 13.0psi @17:15:49.29 SS.reconfigure :inhaling 0.59ml/s @17:15:49.34 SS.seek 25ml @17:16:09.90 getPMLReagent08 @17:16:09.91 PRV.dial :air @17:16:10.38 delay 5 seconds @17:16:15.40 PTV.dial :PRVmixing @17:16:16.76 delay 5 seconds @17:16:21.78 PRV.dial :ab2 @17:16:22.24 PTV.dial :PRVmixing @17:16:22.32 PSV.dial :mixloop @17:16:22.78 PS.reconfigure :default @17:16:22.83 PS.seek 0.3ml @17:16:26.71 delay 2 seconds @17:16:28.73 PRV.dial :air @17:16:29.27 PS.seek 0.55ml @17:16:32.65 addPMLReagent08 @17:16:32.66 PSV.dial :air @17:16:33.12 PS.seek 2ml @17:16:34.39 Stroke completed -- waiting for pressure to equalize ... @17:16:34.40 SS.reconfigure :exhale @17:16:34.45 SS.hold @17:16:48.71 PSV.dial :PML @17:16:49.18 PTV.dial :mixing @17:16:49.94 PBV.dial :waste1 @17:16:50.38 PS.seek 1.85ml @17:16:52.71 PBV.dial between PSV and waste1 @17:16:53.09 PS.seek 0ml @17:16:59.01 Intake.select :close @17:16:59.17 SSV.dial :exhaust @17:16:59.95 Exhaust.select :open @17:17:00.07 SS.seek 0ml @17:17:08.84 Exhaust.select :close @17:17:08.95 SSV.dial CBV @17:17:08.97 CBV.dial SSV @17:17:09.76 Allowing 130s for 25.0ml of remaining 725.0ml @17:17:09.78 Intake.select :open @17:17:11.94 Intake @15.9psia, vacuum from 10.0 to 13.0psi @17:17:11.96 SS.reconfigure :inhaling 0.59ml/s @17:17:12.47 SS.seek 25ml @17:17:12.77 delay 2 seconds @17:17:14.79 delay 8:00 @17:17:56.19 Vacuum too high -- waiting up to 40 seconds @17:17:56.21 SS.reconfigure :exhale @17:17:56.26 SS.hold @17:18:10.81 Intake @17.6psia, vacuum from 10.0 to 13.0psi @17:18:10.83 SS.reconfigure :inhaling 0.47ml/s @17:18:10.88 SS.seek 25ml @17:18:12.66 Stroke completed -- waiting for pressure to equalize ... @17:18:12.67 SS.reconfigure :exhale @17:18:12.72 SS.hold @17:18:24.13 Intake.select :close @17:18:24.28 SSV.dial :exhaust @17:18:25.04 Exhaust.select :open @17:18:25.16 SS.seek 0ml @17:18:33.95 Exhaust.select :close @17:18:34.06 SSV.dial CBV @17:18:34.08 CBV.dial SSV @17:18:34.86 Allowing 130s for 25.0ml of remaining 700.0ml @17:18:34.87 Intake.select :open @17:18:37.04 Intake @15.9psia, vacuum from 10.0 to 13.0psi @17:18:37.06 SS.reconfigure :inhaling 0.47ml/s @17:18:37.11 SS.seek 25ml @17:19:32.46 Stroke completed -- waiting for pressure to equalize ... @17:19:32.47 SS.reconfigure :exhale @17:19:32.52 SS.hold @17:19:59.54 Intake.select :close @17:19:59.70 SSV.dial :exhaust @17:20:00.49 Exhaust.select :open @17:20:00.61 SS.seek 0ml @17:20:09.63 Exhaust.select :close @17:20:09.74 SSV.dial CBV @17:20:09.76 CBV.dial SSV @17:20:10.56 Allowing 130s for 25.0ml of remaining 675.0ml @17:20:10.57 Intake.select :open @17:20:12.74 Intake @15.6psia, vacuum from 10.0 to 13.0psi @17:20:12.76 SS.reconfigure :inhaling 0.47ml/s @17:20:12.81 SS.seek 25ml @17:21:08.54 Stroke completed -- waiting for pressure to equalize ... @17:21:08.55 SS.reconfigure :exhale @17:21:08.60 SS.hold @17:21:37.71 Intake.select :close @17:21:37.87 SSV.dial :exhaust @17:21:38.65 Exhaust.select :open @17:21:38.77 SS.seek 0ml @17:21:47.57 Exhaust.select :close @17:21:47.68 SSV.dial CBV @17:21:47.71 CBV.dial SSV @17:21:48.51 Allowing 130s for 25.0ml of remaining 650.0ml @17:21:48.53 Intake.select :open @17:21:50.69 Intake @15.9psia, vacuum from 10.0 to 13.0psi @17:21:50.71 SS.reconfigure :inhaling 0.47ml/s @17:21:50.76 SS.seek 25ml @17:22:40.97 Vacuum too high -- waiting up to 40 seconds @17:22:40.98 SS.reconfigure :exhale @17:22:41.04 SS.hold @17:22:56.02 Intake @17.1psia, vacuum from 10.0 to 13.0psi @17:22:56.04 SS.reconfigure :inhaling 0.38ml/s @17:22:56.09 SS.seek 25ml @17:23:02.66 Stroke completed -- waiting for pressure to equalize ... @17:23:02.67 SS.reconfigure :exhale @17:23:02.72 SS.hold @17:23:19.05 Intake.select :close @17:23:19.21 SSV.dial :exhaust @17:23:19.97 Exhaust.select :open @17:23:20.09 SS.seek 0ml @17:23:28.94 Exhaust.select :close @17:23:29.52 SSV.dial CBV @17:23:29.55 CBV.dial SSV @17:23:30.35 Allowing 130s for 25.0ml of remaining 625.0ml @17:23:30.37 Intake.select :open @17:23:32.53 Intake @15.9psia, vacuum from 10.0 to 13.0psi @17:23:32.55 SS.reconfigure :inhaling 0.38ml/s @17:23:32.60 SS.seek 25ml @17:24:41.21 Stroke completed -- waiting for pressure to equalize ... @17:24:41.22 SS.reconfigure :exhale @17:24:41.27 SS.hold @17:25:10.38 Intake.select :close @17:25:10.54 SSV.dial :exhaust @17:25:11.30 Exhaust.select :open @17:25:11.42 SS.seek 0ml @17:25:14.81 recoverPRVreagent08 @17:25:14.82 PTV.dial :mixing @17:25:14.89 PBV.dial :waste1 @17:25:15.26 delay 30 seconds @17:25:20.19 Exhaust.select :close @17:25:20.31 SSV.dial CBV @17:25:20.35 CBV.dial SSV @17:25:21.12 Allowing 130s for 25.0ml of remaining 600.0ml @17:25:21.13 Intake.select :open @17:25:23.31 Intake @15.8psia, vacuum from 10.0 to 13.0psi @17:25:23.32 SS.reconfigure :inhaling 0.38ml/s @17:25:23.37 SS.seek 25ml @17:25:45.30 PBV.dial between PSV and waste1 @17:25:45.67 PSV.dial :air @17:25:46.14 PS.reconfigure :fast @17:25:46.19 PS.seek 2.5ml @17:26:10.32 PSV.dial :mixloop @17:26:10.79 delay 2 seconds @17:26:12.81 PSV.dial :air @17:26:13.28 delay 2 seconds @17:26:15.30 PSV.dial :mixloop @17:26:15.76 delay 2 seconds @17:26:17.78 PSV.dial :air @17:26:18.25 PS.seek 0ml @17:26:32.24 Stroke completed -- waiting for pressure to equalize ... @17:26:32.25 SS.reconfigure :exhale @17:26:32.30 SS.hold @17:26:41.82 washCycleDA #1 of 3 @17:26:41.83 PSR.select :air @17:26:41.85 PSV.dial PSR @17:26:43.21 Pulling 0.5ml of PSR.wash @17:26:43.22 PSR.select :wash @17:26:43.34 PS.reconfigure :default @17:26:43.39 PS.seek 0.5ml @17:26:49.26 delay 5 seconds @17:26:54.28 PSR.select :air @17:26:54.39 PS.seek 2ml @17:27:05.10 Intake.select :close @17:27:05.26 SSV.dial :exhaust @17:27:06.02 Exhaust.select :open @17:27:06.14 SS.seek 0ml @17:27:10.60 PTV.dial :puck @17:27:11.35 PSV.dial PTV @17:27:12.57 PBV.dial :waste1 @17:27:13.01 PS.seek 1.5ml @17:27:14.96 Exhaust.select :close @17:27:15.07 SSV.dial CBV @17:27:15.09 CBV.dial SSV @17:27:15.88 Allowing 130s for 25.0ml of remaining 575.0ml @17:27:15.89 Intake.select :open @17:27:18.06 Intake @15.8psia, vacuum from 10.0 to 13.0psi @17:27:18.08 SS.reconfigure :inhaling 0.38ml/s @17:27:18.13 SS.seek 25ml @17:27:18.98 delay 3 seconds @17:27:22.00 PBV.dial between PSV and waste1 @17:27:22.38 PS.seek 0ml @17:27:38.73 delay 2:00 @17:28:26.87 Stroke completed -- waiting for pressure to equalize ... @17:28:26.89 SS.reconfigure :exhale @17:28:26.93 SS.hold @17:29:07.12 SS.reconfigure :default @17:29:07.18 SS.hold @17:29:07.24 Intake.select :close @17:29:07.40 ShallowSampler::Clogged in WCR -- Intake @17psia & Outlet @14psia won't equalize after 450.0ml @17:29:07.42 SSV.dial :exhaust @17:29:08.17 Exhaust.select :open @17:29:08.29 SS.reconfigure :exhale @17:29:08.34 SS.seek 0ml @17:29:17.20 Exhaust.select :close @17:29:17.31 Sampled 450.0ml @17:29:17.33 Drying puck before fixing for 5:00 @17:29:17.34 CBV.dial :waste2b @17:29:18.11 delay 1:00 @17:29:38.75 recoverPSRreagent08 @17:29:38.76 PTV.dial :puck @17:29:38.84 PBV.dial :waste1 @17:29:39.21 delay 30 seconds @17:30:09.24 PBV.dial between PSV and waste1 @17:30:09.62 PSR.select :air @17:30:09.64 PSV.dial PSR @17:30:10.40 PS.reconfigure :fast @17:30:10.45 PS.seek 2.5ml @17:30:18.13 CSV.dial :air @17:30:18.59 CS.reconfigure :fast @17:30:18.65 CS.seek 10ml @17:30:34.51 PSV.dial PTV @17:30:35.40 delay 5 seconds @17:30:40.42 PSV.dial PSR @17:30:41.63 delay 5 seconds @17:30:43.45 Pumping wcr puck from tube 4 up to 35psi... @17:30:43.47 CBV.dial SSV @17:30:44.74 CBV.dial between CSV and waste2b @17:30:45.72 CSV.dial between air and CTV @17:30:46.03 CS.seek 7.248ml @17:30:46.65 PSV.dial PTV @17:30:47.41 delay 5 seconds @17:30:52.43 PSV.dial :air @17:30:53.20 PS.seek 0ml @17:30:53.28 CTV.dial CSV,avoiding: :air @17:30:53.30 CSV.dial CTV @17:30:54.16 CS.reconfigure :pressurize @17:30:54.21 CS.seek 0ml @17:31:12.47 CBV.dial :waste2b @17:31:12.80 CS.reconfigure :default @17:31:12.85 CS.seek 0ml @17:31:14.51 delay 1:00 @17:31:16.57 washCycleDA #2 of 3 @17:31:16.58 PSR.select :air @17:31:16.60 PSV.dial PSR @17:31:17.94 Pulling 0.5ml of PSR.wash @17:31:17.95 PSR.select :wash @17:31:18.07 PS.reconfigure :default @17:31:18.12 PS.seek 0.5ml @17:31:24.07 delay 5 seconds @17:31:29.09 PSR.select :air @17:31:29.21 PS.seek 2ml @17:31:45.41 PTV.dial :puck @17:31:45.49 PSV.dial PTV @17:31:46.24 PBV.dial :waste1 @17:31:46.67 PS.seek 1.5ml @17:31:52.60 delay 3 seconds @17:31:55.62 PBV.dial between PSV and waste1 @17:31:55.98 PS.seek 0ml @17:32:12.10 delay 2:00 @17:32:14.53 CTV.dial :air,avoiding: :intake @17:32:15.86 Fix #1 of 2 (with RNAlater) for 2:30 @17:32:15.87 CSV.dial CSR @17:32:16.32 Pulling 2ml of CSR.RNAlater @17:32:16.33 CSR.select :RNAlater @17:32:16.45 CS.seek 2ml @17:32:22.60 delay 5 seconds @17:32:27.62 CSR.select :air @17:32:27.74 CS.reconfigure :fast @17:32:27.79 CS.seek 10ml @17:32:47.70 Pumping wcr puck from tube 4 up to 35psi... @17:32:47.72 CBV.dial SSV @17:32:49.45 CBV.dial between CSV and waste2b @17:32:50.43 CSV.dial between air and CTV @17:32:51.03 CS.seek 7.103ml @17:32:58.70 CTV.dial CSV,avoiding: :air @17:32:58.73 CSV.dial CTV @17:33:00.14 CS.reconfigure :pressurize @17:33:00.19 CS.seek 0ml @17:33:14.06 delay 2:30 @17:34:12.13 recoverPSRreagent08 @17:34:12.14 PTV.dial :puck @17:34:12.21 PBV.dial :waste1 @17:34:12.58 delay 30 seconds @17:34:42.61 PBV.dial between PSV and waste1 @17:34:42.99 PSR.select :air @17:34:43.01 PSV.dial PSR @17:34:43.77 PS.reconfigure :fast @17:34:43.82 PS.seek 2.5ml @17:35:07.78 PSV.dial PTV @17:35:08.68 delay 5 seconds @17:35:13.70 PSV.dial PSR @17:35:14.44 delay 5 seconds @17:35:19.46 PSV.dial PTV @17:35:20.22 delay 5 seconds @17:35:25.24 PSV.dial :air @17:35:26.01 PS.seek 0ml @17:35:44.08 CBV.dial :waste2b @17:35:44.43 CS.reconfigure :default @17:35:44.48 CS.seek 0ml @17:35:49.39 washCycleDA #3 of 3 @17:35:49.40 PSR.select :air @17:35:49.41 PSV.dial PSR @17:35:49.97 delay 1:00 @17:35:50.75 Pulling 0.5ml of PSR.wash @17:35:50.76 PSR.select :wash @17:35:50.88 PS.reconfigure :default @17:35:50.93 PS.seek 0.5ml @17:35:56.85 delay 5 seconds @17:36:01.87 PSR.select :air @17:36:01.98 PS.seek 2ml @17:36:18.16 PTV.dial :puck @17:36:18.23 PSV.dial PTV @17:36:18.98 PBV.dial :waste1 @17:36:19.42 PS.seek 1.5ml @17:36:25.34 delay 3 seconds @17:36:28.36 PBV.dial between PSV and waste1 @17:36:28.74 PS.seek 0ml @17:36:44.88 delay 2:00 @17:36:50.00 CTV.dial :air,avoiding: :intake @17:36:51.32 Fix #2 of 2 (with RNAlater) for 2:30 @17:36:51.34 CSV.dial CSR @17:36:52.26 Pulling 2ml of CSR.RNAlater @17:36:52.27 CSR.select :RNAlater @17:36:52.39 CS.seek 2ml @17:36:58.35 delay 5 seconds @17:37:03.37 CSR.select :air @17:37:03.49 CS.reconfigure :fast @17:37:03.55 CS.seek 10ml @17:37:23.41 Pumping wcr puck from tube 4 up to 35psi... @17:37:23.43 CBV.dial SSV @17:37:24.68 CBV.dial between CSV and waste2b @17:37:25.66 CSV.dial between air and CTV @17:37:26.26 CS.seek 7.118ml @17:37:33.67 CTV.dial CSV,avoiding: :air @17:37:33.72 CSV.dial CTV @17:37:35.06 CS.reconfigure :pressurize @17:37:35.11 CS.seek 0ml @17:37:53.00 delay 2:30 @17:38:44.90 recoverPSRreagent08 @17:38:44.91 PTV.dial :puck @17:38:44.98 PBV.dial :waste1 @17:38:45.36 delay 30 seconds @17:39:15.39 PBV.dial between PSV and waste1 @17:39:15.81 PSR.select :air @17:39:15.83 PSV.dial PSR @17:39:16.60 PS.reconfigure :fast @17:39:16.65 PS.seek 2.5ml @17:39:40.50 PSV.dial PTV @17:39:41.39 delay 5 seconds @17:39:46.41 PSV.dial PSR @17:39:47.16 delay 5 seconds @17:39:52.18 PSV.dial PTV @17:39:52.94 delay 5 seconds @17:39:57.96 PSV.dial :air @17:39:58.72 PS.seek 0ml @17:40:21.87 Gate.power :camera,:ON @17:40:21.89 Mixing DA Substrates PSR.select :air @17:40:21.91 PSV.dial PSR @17:40:23.06 CBV.dial :waste2b @17:40:23.31 Pulling 0.25ml of PSR.sub1 @17:40:23.34 PSR.select :sub1 @17:40:23.56 CS.reconfigure :default @17:40:23.67 PS.reconfigure :default @17:40:23.76 CS.seek 0ml @17:40:23.96 PS.seek 0.25ml @17:40:25.38 delay 1:00 @17:40:27.41 delay 5 seconds @17:40:32.43 Pulling 0.25ml of PSR.sub2 @17:40:32.45 PSR.select :sub2 @17:40:32.56 PS.seek 0.5ml @17:40:35.87 delay 5 seconds @17:40:40.89 PSR.select :air @17:40:41.00 PS.seek 2ml @17:40:57.24 PTV.dial :puck @17:40:57.32 PSV.dial PTV @17:40:58.11 PBV.dial :waste1 @17:40:58.56 PS.seek 1.5ml @17:41:04.47 delay 3 seconds @17:41:07.48 PBV.dial between PSV and waste1 @17:41:07.88 PS.seek 0ml @17:41:23.93 delay 10 seconds @17:41:25.40 CTV.dial :air,avoiding: :intake @17:41:33.96 recoverPSRreagent08 @17:41:33.97 PTV.dial :puck @17:41:34.04 PBV.dial :waste1 @17:41:34.42 delay 30 seconds @17:42:04.44 PBV.dial between PSV and waste1 @17:42:04.82 PSR.select :air @17:42:04.84 PSV.dial PSR @17:42:05.63 PS.reconfigure :fast @17:42:05.68 PS.seek 2.5ml @17:42:30.09 PSV.dial PTV @17:42:31.00 delay 5 seconds @17:42:36.02 PSV.dial PSR @17:42:36.78 delay 5 seconds @17:42:41.80 PSV.dial PTV @17:42:42.57 delay 5 seconds @17:42:47.59 PSV.dial :air @17:42:48.38 PS.seek 0ml @17:43:12.37 Additional air clear @17:43:12.38 PSR.select :air @17:43:12.40 PSV.dial PSR @17:43:13.76 Pulling 2ml of PSR.air @17:43:13.77 PSR.select :air @17:43:13.79 PS.reconfigure :default @17:43:13.84 PS.seek 2ml @17:43:35.15 delay 5 seconds @17:43:40.17 PTV.dial :puck @17:43:40.24 PSV.dial PTV @17:43:41.01 PBV.dial :waste1 @17:43:41.44 PS.seek 1.5ml @17:43:47.46 delay 3 seconds @17:43:50.48 PBV.dial between PSV and waste1 @17:43:50.85 delay 1 second @17:43:51.87 PSV.dial :waste1 @17:43:52.95 PS.seek 0ml @17:44:09.36 Processing Puck Heater at 28C before imaging @17:44:09.37 PH.coast @17:44:09.47 Imaging da puck in Processing Clamp while storing it to tube 3 @17:44:09.65 SC.reconfigure :tight @17:44:09.69 SC.seek 3 @17:44:13.27 SC.configure :limp @17:44:13.48 Elbow.reconfigure :tight @17:44:13.53 Elbow.seek 3 @17:44:15.28 Forearm.reconfigure :tight @17:44:15.82 Forearm.seek 3 + 450 counts @17:44:16.86 SE.reconfigure :pick @17:44:16.92 SE.seek :beamBreak @17:44:18.85 SE.configure :default @17:44:19.02 Elbow.reconfigure :limp @17:44:19.07 Forearm.reconfigure :loose @17:44:19.12 Forearm.seek 3 @17:44:19.40 Elbow.hold @17:44:19.46 Elbow.reconfigure :tight @17:44:19.57 SE.seek 19.11 Pucks below Top Plate @17:44:20.15 Forearm.force -20 @17:44:20.20 Forearm.seek 3 + 317 counts @17:44:20.50 Forearm.reconfigure :tight @17:44:20.55 Forearm.seek 3 + 450 counts @17:44:20.76 SE.seek 20.11 Pucks below Top Plate @17:44:21.32 Forearm.reconfigure :loose @17:44:21.37 Forearm.seek :retract @17:44:22.30 Elbow.seek PC @17:44:24.81 Elbow.reconfigure :loose @17:44:24.91 Forearm.seek PC @17:44:25.89 Hand.close @17:44:26.51 PC.seek :open @17:44:31.33 Forearm.seek :retract @17:44:32.30 Elbow.reconfigure :tight @17:44:32.35 Elbow.seek 3 @17:44:34.97 Forearm.reconfigure :tight @17:44:35.02 Forearm.seek 3 + 450 counts @17:44:36.08 Hand.open @17:44:36.83 SE.seek 19.76 Pucks below Top Plate @17:44:37.05 SE.reconfigure :seal @17:44:37.10 SE.seek 18.79 Pucks below Top Plate @17:44:37.65 Lights, camera, ACTION! @17:44:37.68 PuckCamera: snap -bin=2,2 -auto /var/log/esp/da23nov2917h725ml.tif @17:44:57.16 Waiting >20 seconds for da to relinquish FlushPuck @17:45:00.23 SX HX9/16 (USB:09): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 19.114 seconds /var/log/esp/da23nov2917h725ml.tif: TIFF Upload Complete @17:45:00.25 PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da23nov2917h725ml15s.tif @17:45:15.91 SX HX9/16 (USB:09): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15 seconds /var/log/esp/midres/da23nov2917h725ml15s.tif: TIFF Upload Complete @17:45:15.94 PuckCamera: snap -bin=2,2 20.0 /var/log/esp/midres/da23nov2917h725ml20s.tif @17:45:36.55 SX HX9/16 (USB:09): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 20 seconds /var/log/esp/midres/da23nov2917h725ml20s.tif: TIFF Upload Complete @17:45:36.57 Gate.power :camera,:OFF @17:45:36.59 DAfans.email "19.114 sec auto-exposure",Subject: "da23nov2917h725ml images!" @17:45:37.05 Elbow.seek 3 @17:45:37.28 Forearm.seek 3 + 450 counts @17:45:37.46 SE.reconfigure :gently @17:45:37.53 SE.seek 19.51 Pucks below Top Plate @17:45:38.18 Elbow.reconfigure :limp @17:45:38.23 Forearm.reconfigure :loose @17:45:38.30 Forearm.seek 3 @17:45:38.58 Elbow.hold @17:45:38.65 Elbow.reconfigure :tight @17:45:38.70 SE.reconfigure :default @17:45:38.75 SE.seek 19.69 Pucks below Top Plate @17:45:39.23 Forearm.force -20 @17:45:39.27 Forearm.seek 3 + 301 counts @17:45:39.60 Forearm.reconfigure :tight @17:45:39.77 Forearm.seek 3 + 450 counts @17:45:40.14 SE.seek :down @17:45:41.40 Forearm.reconfigure :loose @17:45:41.51 Forearm.seek :retract @17:45:41.70 SE.stop @17:45:42.64 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @17:45:43.21 Elbow.seek FlushPuck::Garage @17:45:45.24 Elbow.reconfigure :loose @17:45:45.46 Forearm.seek :garage @17:45:46.48 Hand.close @17:45:48.38 Forearm.seek :retract @17:45:49.38 Elbow.reconfigure :tight @17:45:49.47 Elbow.seek PC @17:45:50.89 Elbow.reconfigure :loose @17:45:51.11 Forearm.reconfigure :tight @17:45:51.21 Forearm.seek PC @17:45:52.83 PC.seek :closed @17:45:57.78 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.65 @17:45:57.92 Hand.open @17:45:58.64 Forearm.reconfigure :loose @17:45:58.73 Forearm.seek :retract @17:45:59.73 PSR.select :air @17:45:59.77 Storing wcr puck in Collection Clamp to tube 3 @17:45:59.82 PSV.dial PSR @17:46:00.71 Pulling 0.5ml of PSR.flush @17:46:00.78 PSR.select :flush @17:46:00.94 Elbow.reconfigure :tight @17:46:01.04 Elbow.seek 3 @17:46:01.17 PS.seek 0.5ml @17:46:04.07 Forearm.reconfigure :tight @17:46:04.19 Forearm.seek 3 + 450 counts @17:46:05.46 SE.reconfigure :pick @17:46:05.57 SE.seek :beamBreak @17:46:07.10 delay 5 seconds @17:46:08.37 SE.configure :default @17:46:08.70 Elbow.reconfigure :limp @17:46:08.81 Forearm.reconfigure :loose @17:46:08.90 Forearm.seek 3 @17:46:09.20 Elbow.hold @17:46:09.33 Elbow.reconfigure :tight @17:46:09.54 SE.seek 20.11 Pucks below Top Plate @17:46:10.20 Forearm.force -20 @17:46:10.28 Forearm.seek 3 + 320 counts @17:46:10.76 Forearm.reconfigure :tight @17:46:10.85 Forearm.seek 3 + 450 counts @17:46:11.13 SE.seek 21.11 Pucks below Top Plate @17:46:11.86 Forearm.reconfigure :loose @17:46:11.92 Forearm.seek :retract @17:46:12.05 upsync @17:46:12.87 Elbow.seek CC @17:46:13.22 PSR.select :air @17:46:13.33 PS.seek 2.5ml @17:46:14.50 Elbow.reconfigure :loose @17:46:14.60 Forearm.seek CC @17:46:15.58 Hand.close @17:46:16.26 CC.seek :open @17:46:21.01 Forearm.seek :retract @17:46:22.02 Elbow.reconfigure :tight @17:46:22.07 Elbow.seek 3 @17:46:23.84 Elbow.reconfigure :limp @17:46:24.28 Forearm.seek 3 @17:46:25.33 Elbow.hold @17:46:25.40 Elbow.reconfigure :tight @17:46:25.45 Hand.open @17:46:26.13 Forearm.force -20 @17:46:26.17 Forearm.seek 3 + 317 counts @17:46:26.47 Forearm.reconfigure :tight @17:46:26.53 Forearm.seek 3 + 450 counts @17:46:26.78 SE.seek :down @17:46:27.91 Forearm.reconfigure :loose @17:46:27.96 Forearm.seek :retract @17:46:28.06 SE.stop @17:46:28.92
Starting finalPurge @17:46:34.55 delay 1 second @17:46:35.57 PRV.dial :waste2,avoiding: :ab2 @17:46:37.44 PTV.dial :PRVmixing @17:46:38.77 PSV.dial :mixloop @17:46:39.85 PS.seek :empty @17:46:48.36
Waiting >20 seconds for psFlush to relinquish FlushPuck @17:47:06.21 PSR.select :air @17:47:06.23 PSV.dial PSR @17:47:07.29 Pulling 0.5ml of PSR.flush @17:47:07.30 PSR.select :flush @17:47:07.41 PS.seek 0.5ml @17:47:13.40 delay 5 seconds @17:47:18.42 PSR.select :air @17:47:18.54 PS.seek 2.5ml @17:47:39.90 delay 1 second @17:47:40.91 PBV.dial :waste1 @17:47:41.29 PTV.dial :mixing @17:47:42.04 PSV.dial :mixloop @17:47:43.10 PS.seek :empty @17:48:09.55 PSV.dial PSR @17:48:10.60 PSR.select :flush @17:48:10.72 PS.seek 1ml @17:48:21.80 PSR.select :air @17:48:21.91 PS.seek 2.5ml @17:48:38.08 PSV.dial PTV @17:48:38.85 PTV.dial :puck @17:48:39.60 PBV.dial :waste1 @17:48:40.12 PS.seek 0ml @17:49:06.46 PSV.dial PSR @17:49:07.21 PSR.select :flush @17:49:07.33 PS.seek 0.5ml @17:49:13.33 PSR.select :air @17:49:13.45 PS.seek 2.5ml @17:49:34.77 PSV.dial PTV @17:49:35.53 PBV.dial PSV @17:49:36.10 PS.seek 0ml @17:50:02.52 PSV.dial PBV @17:50:02.99 PS.seek 2.5ml @17:50:29.39 PSV.dial :waste1 @17:50:30.26 PS.seek 0ml @17:50:56.61 PSV.dial PSR @17:50:57.07 PSR.select :air @17:50:57.09 PS.seek 2.5ml @17:51:23.51 PSV.dial PTV @17:51:24.27 PBV.dial PSV @17:51:24.35 PS.seek 0ml @17:51:50.76 PSV.dial PBV @17:51:51.24 PS.seek 2.5ml @17:52:17.64 PSV.dial :waste1 @17:52:18.40 PS.seek 0ml @17:52:44.95 PSV.dial PSR @17:52:45.42 PSR.select :flush @17:52:45.54 PS.seek 1ml @17:52:56.51 PSR.select :air @17:52:56.63 PS.seek 2.5ml @17:53:12.79 PSV.dial PTV @17:53:13.53 PTV.dial :puck @17:53:13.61 PBV.dial :waste1 @17:53:14.18 PS.seek 0ml @17:53:40.61 PSV.dial PSR @17:53:41.36 PSR.select :flush @17:53:41.48 PS.seek 0.5ml @17:53:47.39 PSR.select :air @17:53:47.50 PS.seek 2.5ml @17:54:08.76 PSV.dial PTV @17:54:09.53 PBV.dial PSV @17:54:10.10 PS.seek 0ml @17:54:36.39 PSV.dial PBV @17:54:36.86 PS.seek 2.5ml @17:55:03.64 PSV.dial :waste1 @17:55:04.46 PS.seek 0ml @17:55:30.82 PSV.dial PSR @17:55:31.29 PSR.select :air @17:55:31.32 PS.seek 2.5ml @17:55:57.82 PSV.dial PTV @17:55:58.57 PBV.dial PSV @17:55:58.66 PS.seek 0ml @17:56:25.01 PSV.dial PBV @17:56:25.49 PS.seek 2.5ml @17:56:51.79 PSV.dial :waste1 @17:56:52.55 PS.seek 0ml @17:57:18.89
Loading FlushPuck into Collection Clamp from Processing Clamp @17:57:19.07 Elbow.seek PC @17:57:21.82 Elbow.reconfigure :loose @17:57:21.92 Forearm.seek PC @17:57:22.96 Hand.close @17:57:23.62 PC.seek :open @17:57:28.38 Forearm.seek :retract @17:57:29.37 Elbow.reconfigure :tight @17:57:29.42 Elbow.seek CC @17:57:32.96 Elbow.reconfigure :loose @17:57:33.05 Forearm.reconfigure :tight @17:57:33.10 Forearm.seek CC @17:57:34.44 CC.seek :closed @17:57:38.96 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.40 @17:57:39.03 Hand.open @17:57:40.11 Forearm.reconfigure :loose @17:57:40.18 Forearm.seek :retract @17:57:41.19 Flushing the Collection Valve::Manifold @17:57:41.31 CSV.dial CSR @17:57:41.77 Pulling 5ml of CSR.flush @17:57:41.78 CSR.select :flush @17:57:41.90 CS.seek 5ml @17:57:55.58 delay 5 seconds @17:58:00.60 CSR.select :air @17:58:00.77 CS.reconfigure :fast @17:58:00.81 CS.seek 7.5ml @17:58:07.55 CSV.dial :waste @17:58:08.85 CS.reconfigure :default @17:58:08.90 CS.seek 0ml @17:58:29.11 Flushing lower manifold's waste1 port @17:58:29.23 CSV.dial :flush @17:58:29.68 CS.reconfigure :fast @17:58:29.73 CS.seek 5ml @17:58:42.45 delay 5 seconds @17:58:47.47 CSV.dial :air @17:58:48.00 CS.seek 7ml @17:58:53.52 CSV.dial CTV @17:58:53.54
CTV.dial CSV @17:58:54.94 CBV.dial :waste1 @17:58:55.71 CS.seek 0ml @17:59:12.27 Flushing lower manifold's waste2 port @17:59:12.39 CSV.dial :flush @17:59:13.12 CS.seek 5ml @17:59:26.05 delay 5 seconds @17:59:31.07 CSV.dial :air @17:59:31.58 CS.seek 7ml @17:59:37.11 CSV.dial CTV @17:59:37.14
CTV.dial CSV @17:59:37.61 CBV.dial :waste2 @17:59:38.66 CS.seek 0ml @17:59:55.30 Flushing lower manifold's waste2b port @17:59:55.47 CSV.dial :flush @17:59:56.21 CS.seek 5ml @18:00:09.33 delay 5 seconds @18:00:14.35 CSV.dial :air @18:00:14.87 CS.seek 7ml @18:00:20.36 CSV.dial CTV @18:00:20.38
CTV.dial CSV @18:00:20.95 CBV.dial :waste2b @18:00:22.01 CS.seek 0ml @18:00:38.80 Disinfecting external Intake with kill @18:00:39.36 CSV.dial CSR @18:00:39.94 Pulling 8ml of CSR.kill @18:00:39.95 CSR.select :kill @18:00:40.07 CS.seek 8ml @18:00:59.89 delay 5 seconds @18:01:04.91 CSR.select :air @18:01:05.03 CS.seek 10ml @18:01:10.62 CSV.dial CBV @18:01:10.64
CBV.dial CSV @18:01:11.72 CTV.dial :intake @18:01:12.48 Intake.select :open @18:01:12.60 CS.seek 2ml @18:01:31.48 Intake.select :close @18:01:31.59 CTV.dial :air @18:01:32.33 CS.reconfigure :default @18:01:32.38 CS.seek 0ml @18:01:38.32 CSV.dial CSR @18:01:39.34 Pulling 5ml of CSR.flush @18:01:39.35 CSR.select :flush @18:01:39.46 CS.seek 5ml @18:01:53.26 delay 5 seconds @18:01:58.28 CSR.select :air @18:01:58.40 CS.seek 10ml @18:02:11.98 CSV.dial CBV @18:02:13.00 CS.seek 0ml @18:02:39.61 Garaging FlushPuck in Collection Clamp @18:02:39.68 Elbow.reconfigure :tight @18:02:39.73 Elbow.seek CC @18:02:40.00 Elbow.reconfigure :loose @18:02:40.10 Forearm.seek CC @18:02:41.12 Hand.close @18:02:41.75 CC.seek :open @18:02:46.38 Forearm.seek :retract @18:02:47.40 Elbow.reconfigure :tight @18:02:47.45 Elbow.seek :garage @18:02:50.31 Elbow.reconfigure :loose @18:02:50.41 Forearm.reconfigure :tight @18:02:50.46 Forearm.seek :garage @18:02:51.51 Hand.open @18:02:52.18 Forearm.reconfigure :loose @18:02:52.22 Forearm.seek :retract @18:02:53.21 Completed [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] begun 15:39:08 @18:02:53.26 Asleep after completing DA_WCR_112923 assays @18:02:53.28 GoodNews.email "{1 => 33,\n 2 => 33,\n 3 => 22,\n 4 => 11,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@17:58:30, 30% flow, 17.0C, 29% humidity, 15.2psia, 13.681V, 0.466A, 109.168Ah, 6.37W\nSampled 450.0ml",Subject: "Asleep after completing DA_WCR_112923 assays" @18:02:57.76 upsync + ESPwhich DA_WCR_113023.rb + fn=DA_WCR_113023.rb + IFS=: + set /home/esp/esp2/mission . + fullPath=/home/esp/esp2/mission/DA_WCR_113023.rb + [ -f /home/esp/esp2/mission/DA_WCR_113023.rb ] + realpath /home/esp/esp2/mission/DA_WCR_113023.rb + return 0 + scriptPath=/home/esp/esp2local/mission/DA_WCR_113023.rb + exec ruby /home/esp/esp2/lib/esp.rb /home/esp/esp2local/mission/DA_WCR_113023.rb @13:58:39.55UTC30-Nov-23
ESP2 v3.42 I2C Gateway v3.20 for core bus @13:58:42.22 Configuration for short puck jake 1/11/21 by brent@mbari.org /home/esp/esp2/type/shallow/jake/configure.rb @13:58:45.86 Polling Can every 10:00 @13:58:45.98 Cleanly exited at 13:56:05UTC30-Nov-23 @13:58:46.03 Loading /home/esp/esp2local/mission/DA_WCR_113023.rb mission @13:58:46.09 Preparing to sleep until + 00:00:05 @13:58:46.12 GoodNews.email "Starting DA_WCR_113023 w/pucks in tube 4\n{1 => 33,\n 2 => 33,\n 3 => 22,\n 4 => 11,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@13:58:45, 31% flow, 16.2C, 28% humidity, 15.1psia, 13.734V, 0.296A, 114.689Ah, 4.07W",Subject: "DA_WCR_113023 begun" @13:58:46.17
Asleep til + 00:00:05 @13:58:46.18 Continuing at 13:58 @13:58:46.42 Gate.power :core,:OFF @13:58:46.44 Gate.power :raw,:OFF @13:58:46.45 Shutdown @13:58:48.95 GoodNews.email "Then, in tube 4: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_113023.rb:14\n{1 => 33,\n 2 => 33,\n 3 => 22,\n 4 => 11,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@13:58:46, 31% flow, 16.2C, 28% humidity, 15.1psia, 13.734V, 0.290A, 114.689Ah, 3.99W",Subject: "Asleep until 13:58" @13:58:50.34 upsync @13:58:51.07
Powering core bus @13:58:52.92 upsync @13:58:56.47
Gate.power :raw,:ON @13:58:57.28 CS.configure :default @13:58:57.33 PS.configure :default @13:58:57.38 SS.configure :default @13:58:57.43 CC.configure :default @13:58:57.48 PC.configure :default @13:58:57.55 Forearm.configure :loose @13:58:57.60 Elbow.configure :loose @13:58:57.67 SC.configure :loose @13:58:57.72 SE.configure :default @13:58:57.76 CH.configure :default @13:58:57.80 PH.configure :default @13:58:57.84 All dwarf controllers running firmware version 3.85 @13:58:57.95 Valve.reset @13:58:58.28 PSV.dial :air @13:58:58.32 Intake.select :close @13:58:58.36 Exhaust.select :close @13:58:58.39 CTV.dial :air @13:58:58.47
Forearm.home.seek :retract @13:58:58.53 CSV.dial :waste1 @13:58:58.98 SSV.dial :spit,avoiding: :exhaust @13:58:59.18 Hand.close @13:58:59.21
Elbow.reconfigure :loose @13:58:59.30 Elbow.jog 1500 @13:58:59.39 PS.home.seek 0ml @13:58:59.85 CS.reconfigure :fast @13:58:59.89 SS.home.seek 0ml @13:58:59.98 CS.home.seek 0ml @13:59:00.06
Elbow.reconfigure :loose @13:59:00.11 Elbow.home.seek :clear @13:59:03.47 SE.home.seek :down @13:59:04.09 SC.reconfigure :tight @13:59:04.16 SC.home.seek :initial @13:59:07.66 PS.stop @13:59:19.89
SC.configure :limp @13:59:19.95 Forearm.seek :retract @13:59:20.01 Elbow.seek :hammer @13:59:21.20 Forearm.reconfigure :tight @13:59:21.25 Forearm.seek retracted @13:59:21.96 CC.home.seek :open @13:59:22.04 PC.home.seek :open @13:59:25.48
Phase [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] @13:59:25.49 Notice.email "At 13:59:25 began in tube 4: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_113023.rb:14\n{1 => 33,\n 2 => 33,\n 3 => 22,\n 4 => 11,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@13:59:25, 31% flow, 16.3C, 28% humidity, 15.1psia, 13.681V, 0.266A, 114.693Ah, 3.64W",Subject: "Started DA_WCR_113023 phase initialPurge; da [1000, 1000] #da and wsr are run simultanelously" @13:59:25.52
Starting initialPurge @13:59:25.54 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @13:59:25.60 Hand.open @13:59:26.40 Elbow.reconfigure :tight @13:59:26.45 Elbow.seek FlushPuck::Garage @13:59:28.47 Elbow.reconfigure :loose @13:59:28.69 Forearm.reconfigure :loose @13:59:28.79 Forearm.seek :garage @13:59:29.75 Hand.close @13:59:29.90 upsync @13:59:30.52
Forearm.seek :retract @13:59:31.54 Elbow.reconfigure :tight @13:59:31.59 Elbow.seek CC @13:59:34.60 Elbow.reconfigure :loose @13:59:34.70 Forearm.reconfigure :tight @13:59:34.76 Forearm.seek CC @13:59:36.05 CC.seek :closed @13:59:40.69 Collection Clamp onSpring/offSpring for 14/27 samples, on/off torque 2.39 @13:59:40.77 Hand.open @13:59:41.44 Forearm.reconfigure :loose @13:59:41.50 Forearm.seek :retract @13:59:42.48 Flushing the Collection Valve::Manifold @13:59:42.61 CSV.dial CSR @13:59:43.91 Pulling 4.999ml of CSR.flush @13:59:43.92 CSR.select :flush @13:59:44.04 CS.reconfigure :default @13:59:44.50 CS.seek 5ml @13:59:58.30 delay 5 seconds @14:00:03.32 CSR.select :air @14:00:03.49 CS.reconfigure :fast @14:00:03.54 CS.seek 7.5ml @14:00:10.36 CSV.dial :waste @14:00:11.66 CS.reconfigure :default @14:00:11.71 CS.seek 0ml @14:00:31.93 Purging external Intake @14:00:32.05 CSV.dial :flush @14:00:32.49 CS.reconfigure :fast @14:00:32.55 CS.seek 10ml @14:00:57.11 delay 5 seconds @14:01:02.13 CSV.dial CBV @14:01:02.16
CBV.dial CSV @14:01:02.94 CTV.dial :intake @14:01:03.91 Intake.select :open @14:01:04.02 CS.seek 0ml @14:01:27.92 Intake.select :close @14:01:28.03 CTV.dial :air @14:01:28.75 Flushing upper manifold @14:01:28.88 CSV.dial :flush @14:01:29.63 CS.seek 5ml @14:01:42.45 delay 5 seconds @14:01:47.47 CSV.dial :air @14:01:47.99 CS.seek 7ml @14:01:53.64 CSV.dial CBV @14:01:53.67
CBV.dial CSV @14:01:54.71 CTV.dial :air @14:01:54.73 CS.seek 0ml @14:02:11.49 delay 7 seconds @14:02:18.51 CS.seek 3ml @14:02:26.55 CSV.dial :waste @14:02:27.02 CS.seek 0ml @14:02:34.85 CSV.dial :flush @14:02:35.29 CS.seek 3ml @14:02:43.30 delay 5 seconds @14:02:48.32 CSV.dial :air @14:02:48.94 CS.seek 5ml @14:02:54.51 CSV.dial :air @14:02:54.60 CS.seek 0ml @14:03:06.70 delay 7 seconds @14:03:13.73 CS.seek 3ml @14:03:21.76 CSV.dial :waste @14:03:22.49 CS.seek 0ml @14:03:30.11 Garaging FlushPuck in Collection Clamp @14:03:30.18 Elbow.reconfigure :tight @14:03:30.23 Elbow.seek CC @14:03:30.58 Elbow.reconfigure :loose @14:03:30.73 Forearm.seek CC @14:03:31.82 Hand.close @14:03:32.38 CC.seek :open @14:03:37.44 Forearm.seek :retract @14:03:38.44 Elbow.reconfigure :tight @14:03:38.49 Elbow.seek :garage @14:03:41.31 Elbow.reconfigure :loose @14:03:41.42 Forearm.reconfigure :tight @14:03:41.46 Forearm.seek :garage @14:03:42.51 Hand.open @14:03:43.19 Forearm.reconfigure :loose @14:03:43.24 Forearm.seek :retract @14:03:44.23 da sampling at most 1000ml, wcr at most 1000ml @14:03:44.26 Loading sh1 puck from tube 4 into Collection Clamp @14:03:44.28 DAfans.email "Started da in tube 4 at 14:03:44UTC30-Nov-23\n{1 => 33,\n 2 => 33,\n 3 => 22,\n 4 => 11,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@13:59:25, 31% flow, 16.3C, 28% humidity, 15.1psia, 13.681V, 0.266A, 114.693Ah, 3.64W",Subject: "da sampling at most 1000ml, wcr at most 1000ml" @14:03:44.56
SC.reconfigure :tight @14:03:44.62 SC.seek 4 @14:03:44.75 SC.configure :limp @14:03:45.00 Elbow.reconfigure :tight @14:03:45.06 Elbow.seek 4 @14:03:47.05 Forearm.reconfigure :tight @14:03:47.09 Forearm.seek 4 + 450 counts @14:03:48.13 SE.reconfigure :pick @14:03:48.18 SE.seek :beamBreak @14:03:50.16 upsync @14:03:50.70
SE.configure :default @14:03:50.86 Elbow.seek 4 @14:03:51.06 Forearm.seek 4 + 300 counts @14:03:51.27 Forearm.reconfigure :loose @14:03:51.32 Hand.close @14:03:51.90 SE.seek :down @14:03:52.11
Forearm.seek :retract @14:03:53.14 Elbow.seek CC @14:03:54.01 SE.stop @14:03:54.73
Elbow.reconfigure :loose @14:03:54.84 Forearm.reconfigure :tight @14:03:54.89 Forearm.seek CC @14:03:56.19 CC.seek :closed @14:04:00.75 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.35 @14:04:00.76 Hand.open @14:04:01.44 Forearm.reconfigure :loose @14:04:01.49 Forearm.seek :retract @14:04:02.44 collectSample 1000ml w/bubble point of 24psi @14:04:02.46 Priming 7ml @14:04:02.47 Intake.select :close @14:04:02.48 Exhaust.select :close @14:04:02.49 SS.hold @14:04:02.70 Calibrating pressure and temperature sensors @14:04:02.73 CTV.dial :air @14:04:02.74 SSV.dial :spit,avoiding: :exhaust @14:04:02.83 SS.reconfigure :fast @14:04:02.88 SS.seek 5ml @14:04:05.75 SSV.dial CBV @14:04:05.77
CBV.dial SSV @14:04:07.12 delay 3.8 seconds @14:04:11.05 Sensor limits: Intake max 103.6psia; Outlet max 96.1psia @14:04:11.06 SSV.dial :spit @14:04:12.39 SS.seek 0ml @14:04:15.39 SSV.dial CBV @14:04:16.71 CTV.dial :intake @14:04:17.49 SSV.dial CBV @14:04:17.51
CBV.dial SSV @14:04:17.59 Allowing 40s for 7.0ml of remaining 7.0ml @14:04:17.60 Intake.select :open @14:04:19.78 Intake @14.5psia, vacuum from 9.0 to 12.0psi @14:04:19.79 SS.reconfigure :inhaling 2.20ml/s @14:04:19.84 SS.seek 7ml @14:04:26.31 Stroke completed -- waiting for pressure to equalize ... @14:04:26.32 SS.reconfigure :exhale @14:04:26.36 SS.hold @14:04:32.30 Intake.select :close @14:04:32.46 SSV.dial :exhaust @14:04:33.21 Exhaust.select :open @14:04:33.33 SS.seek 0ml @14:04:37.43 Exhaust.select :close @14:04:37.55 Sampling 1000.0ml @14:04:37.56 Intake.select :close @14:04:37.57 Exhaust.select :close @14:04:37.58 SS.reconfigure :default @14:04:37.63 SS.hold @14:04:37.83 CTV.dial :intake @14:04:38.05 SSV.dial CBV @14:04:38.07
CBV.dial SSV @14:04:38.87 Allowing 130s for 25.0ml of remaining 1000.0ml @14:04:38.88 Intake.select :open @14:04:41.05 Intake @15.1psia, vacuum from 9.6 to 12.6psi @14:04:41.06 SS.reconfigure :inhaling 2.20ml/s @14:04:41.11 SS.seek 25ml @14:04:51.27 Vacuum too high -- waiting up to 40 seconds @14:04:51.28 SS.reconfigure :exhale @14:04:51.34 SS.hold @14:04:59.14 Intake @18.6psia, vacuum from 10.0 to 13.0psi @14:04:59.15 SS.reconfigure :inhaling 1.76ml/s @14:04:59.20 SS.seek 25ml @14:05:02.02 Vacuum too high -- waiting up to 40 seconds @14:05:02.03 SS.reconfigure :exhale @14:05:02.09 SS.hold @14:05:03.96 Intake @17.4psia, vacuum from 10.0 to 13.0psi @14:05:03.97 SS.reconfigure :inhaling 1.41ml/s @14:05:04.02 SS.seek 25ml @14:05:08.21 Vacuum too high -- waiting up to 40 seconds @14:05:08.22 SS.reconfigure :exhale @14:05:08.27 SS.hold @14:05:10.90 Intake @17.9psia, vacuum from 10.0 to 13.0psi @14:05:10.92 SS.reconfigure :inhaling 1.13ml/s @14:05:10.97 SS.seek 25ml @14:05:12.70 Stroke completed -- waiting for pressure to equalize ... @14:05:12.71 SS.reconfigure :exhale @14:05:12.75 SS.hold @14:05:33.15 Intake.select :close @14:05:33.31 SSV.dial :exhaust @14:05:34.08 Exhaust.select :open @14:05:34.19 SS.seek 0ml @14:05:43.01 Exhaust.select :close @14:05:43.12 SSV.dial CBV @14:05:43.14
CBV.dial SSV @14:05:43.93 Allowing 130s for 25.0ml of remaining 975.0ml @14:05:43.94 Intake.select :open @14:05:46.11 Intake @15.7psia, vacuum from 10.0 to 13.0psi @14:05:46.13 SS.reconfigure :inhaling 1.13ml/s @14:05:46.18 SS.seek 25ml @14:06:11.15 Stroke completed -- waiting for pressure to equalize ... @14:06:11.16 SS.reconfigure :exhale @14:06:11.21 SS.hold @14:06:40.17 Intake.select :close @14:06:40.33 SSV.dial :exhaust @14:06:41.09 Exhaust.select :open @14:06:41.20 SS.seek 0ml @14:06:50.02 Exhaust.select :close @14:06:50.13 SSV.dial CBV @14:06:50.15
CBV.dial SSV @14:06:50.96 Allowing 130s for 25.0ml of remaining 950.0ml @14:06:50.97 Intake.select :open @14:06:53.14 Intake @15.8psia, vacuum from 10.0 to 13.0psi @14:06:53.15 SS.reconfigure :inhaling 1.13ml/s @14:06:53.20 SS.seek 25ml @14:07:18.10 Stroke completed -- waiting for pressure to equalize ... @14:07:18.11 SS.reconfigure :exhale @14:07:18.16 SS.hold @14:07:43.34 Intake.select :close @14:07:43.49 SSV.dial :exhaust @14:07:44.26 Exhaust.select :open @14:07:44.38 SS.seek 0ml @14:07:53.19 Exhaust.select :close @14:07:53.30 SSV.dial CBV @14:07:53.34
CBV.dial SSV @14:07:54.10 Allowing 130s for 25.0ml of remaining 925.0ml @14:07:54.11 Intake.select :open @14:07:56.28 Intake @15.9psia, vacuum from 10.0 to 13.0psi @14:07:56.30 SS.reconfigure :inhaling 1.13ml/s @14:07:56.35 SS.seek 25ml @14:08:21.11 Stroke completed -- waiting for pressure to equalize ... @14:08:21.12 SS.reconfigure :exhale @14:08:21.17 SS.hold @14:08:45.74 Intake.select :close @14:08:45.89 SSV.dial :exhaust @14:08:46.65 Exhaust.select :open @14:08:46.76 SS.seek 0ml @14:08:55.52 Exhaust.select :close @14:08:55.63 SSV.dial CBV @14:08:55.66
CBV.dial SSV @14:08:56.46 Allowing 130s for 25.0ml of remaining 900.0ml @14:08:56.47 Intake.select :open @14:08:58.64 Intake @15.9psia, vacuum from 10.0 to 13.0psi @14:08:58.65 SS.reconfigure :inhaling 1.13ml/s @14:08:58.71 SS.seek 25ml @14:09:23.39 Stroke completed -- waiting for pressure to equalize ... @14:09:23.40 SS.reconfigure :exhale @14:09:23.45 SS.hold @14:09:45.90 Intake.select :close @14:09:46.05 SSV.dial :exhaust @14:09:46.82 Exhaust.select :open @14:09:46.98 SS.seek 0ml @14:09:55.80 Exhaust.select :close @14:09:55.91 SSV.dial CBV @14:09:55.93
CBV.dial SSV @14:09:56.74 Allowing 130s for 25.0ml of remaining 875.0ml @14:09:56.75 Intake.select :open @14:09:58.92 Intake @15.8psia, vacuum from 10.0 to 13.0psi @14:09:58.93 SS.reconfigure :inhaling 1.13ml/s @14:09:58.98 SS.seek 25ml @14:10:23.75 Stroke completed -- waiting for pressure to equalize ... @14:10:23.76 SS.reconfigure :exhale @14:10:23.81 SS.hold @14:10:44.19 Intake.select :close @14:10:44.35 SSV.dial :exhaust @14:10:45.54 Exhaust.select :open @14:10:45.66 SS.seek 0ml @14:10:54.44 Exhaust.select :close @14:10:54.55 SSV.dial CBV @14:10:54.57
CBV.dial SSV @14:10:55.36 Allowing 130s for 25.0ml of remaining 850.0ml @14:10:55.38 Intake.select :open @14:10:57.54 Intake @15.9psia, vacuum from 10.0 to 13.0psi @14:10:57.56 SS.reconfigure :inhaling 1.13ml/s @14:10:57.61 SS.seek 25ml @14:11:22.25 Stroke completed -- waiting for pressure to equalize ... @14:11:22.26 SS.reconfigure :exhale @14:11:22.31 SS.hold @14:11:44.75 Intake.select :close @14:11:44.91 SSV.dial :exhaust @14:11:45.69 Exhaust.select :open @14:11:45.81 SS.seek 0ml @14:11:54.75 Exhaust.select :close @14:11:54.86 SSV.dial CBV @14:11:54.88
CBV.dial SSV @14:11:56.21 Allowing 130s for 25.0ml of remaining 825.0ml @14:11:56.22 Intake.select :open @14:11:58.38 Intake @15.9psia, vacuum from 10.0 to 13.0psi @14:11:58.40 SS.reconfigure :inhaling 1.13ml/s @14:11:58.45 SS.seek 25ml @14:12:22.67 Stroke completed -- waiting for pressure to equalize ... @14:12:22.68 SS.reconfigure :exhale @14:12:22.73 SS.hold @14:12:43.12 Intake.select :close @14:12:43.28 SSV.dial :exhaust @14:12:44.03 Exhaust.select :open @14:12:44.15 SS.seek 0ml @14:12:53.03 Exhaust.select :close @14:12:53.14 SSV.dial CBV @14:12:53.16
CBV.dial SSV @14:12:53.97 Allowing 130s for 25.0ml of remaining 800.0ml @14:12:53.98 Intake.select :open @14:12:56.15 Intake @15.9psia, vacuum from 10.0 to 13.0psi @14:12:56.17 SS.reconfigure :inhaling 1.13ml/s @14:12:56.22 SS.seek 25ml @14:13:20.69 Stroke completed -- waiting for pressure to equalize ... @14:13:20.70 SS.reconfigure :exhale @14:13:20.75 SS.hold @14:13:41.56 Intake.select :close @14:13:41.72 SSV.dial :exhaust @14:13:42.49 Exhaust.select :open @14:13:42.61 SS.seek 0ml @14:13:51.36 Exhaust.select :close @14:13:51.47 SSV.dial CBV @14:13:51.49
CBV.dial SSV @14:13:52.28 Allowing 130s for 25.0ml of remaining 775.0ml @14:13:52.29 Intake.select :open @14:13:54.46 Intake @15.9psia, vacuum from 10.0 to 13.0psi @14:13:54.48 SS.reconfigure :inhaling 1.13ml/s @14:13:54.53 SS.seek 25ml @14:14:19.25 Stroke completed -- waiting for pressure to equalize ... @14:14:19.26 SS.reconfigure :exhale @14:14:19.31 SS.hold @14:14:39.86 Intake.select :close @14:14:40.01 SSV.dial :exhaust @14:14:40.77 Exhaust.select :open @14:14:40.89 SS.seek 0ml @14:14:49.81 Exhaust.select :close @14:14:49.92 SSV.dial CBV @14:14:49.94
CBV.dial SSV @14:14:50.75 Allowing 130s for 25.0ml of remaining 750.0ml @14:14:50.76 Intake.select :open @14:14:52.93 Intake @15.8psia, vacuum from 10.0 to 13.0psi @14:14:52.95 SS.reconfigure :inhaling 1.13ml/s @14:14:52.99 SS.seek 25ml @14:15:14.07 Vacuum too high -- waiting up to 40 seconds @14:15:14.08 SS.reconfigure :exhale @14:15:14.13 SS.hold @14:15:21.77 Intake @15.8psia, vacuum from 10.0 to 13.0psi @14:15:21.78 SS.reconfigure :inhaling 0.91ml/s @14:15:21.83 SS.seek 25ml @14:15:25.89 Stroke completed -- waiting for pressure to equalize ... @14:15:25.90 SS.reconfigure :exhale @14:15:25.95 SS.hold @14:15:38.55 Intake.select :close @14:15:38.70 SSV.dial :exhaust @14:15:39.47 Exhaust.select :open @14:15:39.58 SS.seek 0ml @14:15:48.42 Exhaust.select :close @14:15:48.53 SSV.dial CBV @14:15:48.55
CBV.dial SSV @14:15:49.36 Allowing 130s for 25.0ml of remaining 725.0ml @14:15:49.37 Intake.select :open @14:15:51.96 Intake @15.8psia, vacuum from 10.0 to 13.0psi @14:15:51.98 SS.reconfigure :inhaling 0.91ml/s @14:15:52.03 SS.seek 25ml @14:16:21.25 Stroke completed -- waiting for pressure to equalize ... @14:16:21.26 SS.reconfigure :exhale @14:16:21.30 SS.hold @14:16:39.56 Intake.select :close @14:16:39.72 SSV.dial :exhaust @14:16:40.48 Exhaust.select :open @14:16:40.60 SS.seek 0ml @14:16:49.32 Exhaust.select :close @14:16:49.43 SSV.dial CBV @14:16:49.46
CBV.dial SSV @14:16:50.23 Allowing 130s for 25.0ml of remaining 700.0ml @14:16:50.24 Intake.select :open @14:16:52.41 Intake @15.8psia, vacuum from 10.0 to 13.0psi @14:16:52.42 SS.reconfigure :inhaling 0.91ml/s @14:16:52.47 SS.seek 25ml @14:17:22.17 Stroke completed -- waiting for pressure to equalize ... @14:17:22.18 SS.reconfigure :exhale @14:17:22.22 SS.hold @14:17:40.85 Intake.select :close @14:17:41.01 SSV.dial :exhaust @14:17:41.76 Exhaust.select :open @14:17:41.88 SS.seek 0ml @14:17:50.84 Exhaust.select :close @14:17:50.95 SSV.dial CBV @14:17:50.97
CBV.dial SSV @14:17:51.76 Allowing 130s for 25.0ml of remaining 675.0ml @14:17:51.77 Intake.select :open @14:17:53.94 Intake @15.9psia, vacuum from 10.0 to 13.0psi @14:17:53.95 SS.reconfigure :inhaling 0.91ml/s @14:17:54.01 SS.seek 25ml @14:18:21.63 Vacuum too high -- waiting up to 40 seconds @14:18:21.64 SS.reconfigure :exhale @14:18:21.69 SS.hold @14:18:30.41 Intake @16.6psia, vacuum from 10.0 to 13.0psi @14:18:30.42 SS.reconfigure :inhaling 0.73ml/s @14:18:30.47 SS.seek 25ml @14:18:32.77 Stroke completed -- waiting for pressure to equalize ... @14:18:32.78 SS.reconfigure :exhale @14:18:32.83 SS.hold @14:18:43.97 Intake.select :close @14:18:44.13 SSV.dial :exhaust @14:18:44.89 Exhaust.select :open @14:18:45.00 SS.seek 0ml @14:18:53.71 Exhaust.select :close @14:18:53.82 SSV.dial CBV @14:18:53.84
CBV.dial SSV @14:18:54.67 Allowing 130s for 25.0ml of remaining 650.0ml @14:18:54.68 Intake.select :open @14:18:57.27 Intake @15.8psia, vacuum from 10.0 to 13.0psi @14:18:57.28 SS.reconfigure :inhaling 0.73ml/s @14:18:57.33 SS.seek 25ml @14:19:33.70 Stroke completed -- waiting for pressure to equalize ... @14:19:33.71 SS.reconfigure :exhale @14:19:33.75 SS.hold @14:19:49.98 Intake.select :close @14:19:50.13 SSV.dial :exhaust @14:19:50.88 Exhaust.select :open @14:19:51.00 SS.seek 0ml @14:19:59.79 Exhaust.select :close @14:19:59.90 SSV.dial CBV @14:19:59.92
CBV.dial SSV @14:20:00.73 Allowing 130s for 25.0ml of remaining 625.0ml @14:20:00.74 Intake.select :open @14:20:02.90 Intake @16.0psia, vacuum from 10.0 to 13.0psi @14:20:02.92 SS.reconfigure :inhaling 0.73ml/s @14:20:02.97 SS.seek 25ml @14:20:39.05 Stroke completed -- waiting for pressure to equalize ... @14:20:39.06 SS.reconfigure :exhale @14:20:39.11 SS.hold @14:20:57.67 Intake.select :close @14:20:57.82 SSV.dial :exhaust @14:20:58.59 Exhaust.select :open @14:20:58.70 SS.seek 0ml @14:21:07.44 Exhaust.select :close @14:21:07.55 SSV.dial CBV @14:21:07.57
CBV.dial SSV @14:21:08.38 Allowing 130s for 25.0ml of remaining 600.0ml @14:21:08.39 Intake.select :open @14:21:10.56 Intake @16.0psia, vacuum from 10.0 to 13.0psi @14:21:10.58 SS.reconfigure :inhaling 0.73ml/s @14:21:10.62 SS.seek 25ml @14:21:46.90 Stroke completed -- waiting for pressure to equalize ... @14:21:46.91 SS.reconfigure :exhale @14:21:46.95 SS.hold @14:22:07.35 Intake.select :close @14:22:07.50 SSV.dial :exhaust @14:22:08.26 Exhaust.select :open @14:22:08.38 SS.seek 0ml @14:22:17.10 Exhaust.select :close @14:22:17.21 SSV.dial CBV @14:22:17.23
CBV.dial SSV @14:22:18.05 Allowing 130s for 25.0ml of remaining 575.0ml @14:22:18.06 Intake.select :open @14:22:20.23 Intake @16.0psia, vacuum from 10.0 to 13.0psi @14:22:20.25 SS.reconfigure :inhaling 0.73ml/s @14:22:20.30 SS.seek 25ml @14:22:55.18 Vacuum too high -- waiting up to 40 seconds @14:22:55.20 SS.reconfigure :exhale @14:22:55.25 SS.hold @14:23:03.32 Intake @15.7psia, vacuum from 10.0 to 13.0psi @14:23:03.33 SS.reconfigure :inhaling 0.59ml/s @14:23:03.39 SS.seek 25ml @14:23:05.30 Stroke completed -- waiting for pressure to equalize ... @14:23:05.31 SS.reconfigure :exhale @14:23:05.36 SS.hold @14:23:18.05 Intake.select :close @14:23:18.21 SSV.dial :exhaust @14:23:18.97 Exhaust.select :open @14:23:19.09 SS.seek 0ml @14:23:28.07 Exhaust.select :close @14:23:28.17 SSV.dial CBV @14:23:28.19
CBV.dial SSV @14:23:29.01 Allowing 130s for 25.0ml of remaining 550.0ml @14:23:29.02 Intake.select :open @14:23:31.18 Intake @15.9psia, vacuum from 10.0 to 13.0psi @14:23:31.19 SS.reconfigure :inhaling 0.59ml/s @14:23:31.25 SS.seek 25ml @14:24:15.36 Stroke completed -- waiting for pressure to equalize ... @14:24:15.37 SS.reconfigure :exhale @14:24:15.42 SS.hold @14:24:34.18 Intake.select :close @14:24:34.34 SSV.dial :exhaust @14:24:35.11 Exhaust.select :open @14:24:35.23 SS.seek 0ml @14:24:43.96 Exhaust.select :close @14:24:44.06 SSV.dial CBV @14:24:44.08
CBV.dial SSV @14:24:44.89 Allowing 130s for 25.0ml of remaining 525.0ml @14:24:44.91 Intake.select :open @14:24:47.07 Intake @15.8psia, vacuum from 10.0 to 13.0psi @14:24:47.08 SS.reconfigure :inhaling 0.59ml/s @14:24:47.13 SS.seek 25ml @14:25:31.91 Stroke completed -- waiting for pressure to equalize ... @14:25:31.92 SS.reconfigure :exhale @14:25:31.97 SS.hold @14:25:52.48 Intake.select :close @14:25:52.64 SSV.dial :exhaust @14:25:53.40 Exhaust.select :open @14:25:53.52 SS.seek 0ml @14:26:02.25 Exhaust.select :close @14:26:02.37 SSV.dial CBV @14:26:02.39
CBV.dial SSV @14:26:03.19 Allowing 130s for 25.0ml of remaining 500.0ml @14:26:03.20 Intake.select :open @14:26:05.37 Intake @15.9psia, vacuum from 10.0 to 13.0psi @14:26:05.38 SS.reconfigure :inhaling 0.59ml/s @14:26:05.44 SS.seek 25ml @14:26:50.34 Stroke completed -- waiting for pressure to equalize ... @14:26:50.35 SS.reconfigure :exhale @14:26:50.40 SS.hold @14:27:13.26 Intake.select :close @14:27:13.42 SSV.dial :exhaust @14:27:14.17 Exhaust.select :open @14:27:14.29 SS.seek 0ml @14:27:23.05 Exhaust.select :close @14:27:23.15 SSV.dial CBV @14:27:23.17
CBV.dial SSV @14:27:23.97 Allowing 130s for 25.0ml of remaining 475.0ml @14:27:23.98 Intake.select :open @14:27:26.15 Intake @15.8psia, vacuum from 10.0 to 13.0psi @14:27:26.16 SS.reconfigure :inhaling 0.59ml/s @14:27:26.21 SS.seek 25ml @14:28:10.86 Stroke completed -- waiting for pressure to equalize ... @14:28:10.87 SS.reconfigure :exhale @14:28:10.92 SS.hold @14:28:35.35 Intake.select :close @14:28:35.51 SSV.dial :exhaust @14:28:36.28 Exhaust.select :open @14:28:36.40 SS.seek 0ml @14:28:45.36 Exhaust.select :close @14:28:45.47 SSV.dial CBV @14:28:45.49
CBV.dial SSV @14:28:46.30 Allowing 130s for 25.0ml of remaining 450.0ml @14:28:46.31 Intake.select :open @14:28:48.48 Intake @15.8psia, vacuum from 10.0 to 13.0psi @14:28:48.49 SS.reconfigure :inhaling 0.59ml/s @14:28:48.54 SS.seek 25ml @14:29:27.46 Vacuum too high -- waiting up to 40 seconds @14:29:27.47 SS.reconfigure :exhale @14:29:27.52 SS.hold @14:29:36.25 SS.reconfigure :inhaling 0.47ml/s @14:29:36.30 SS.seek 25ml @14:29:43.36 Stroke completed -- waiting for pressure to equalize ... @14:29:43.37 SS.reconfigure :exhale @14:29:43.41 SS.hold @14:30:01.71 Intake.select :close @14:30:01.86 SSV.dial :exhaust @14:30:03.05 Exhaust.select :open @14:30:03.16 SS.seek 0ml @14:30:11.86 Exhaust.select :close @14:30:11.96 SSV.dial CBV @14:30:12.00
CBV.dial SSV @14:30:12.75 Allowing 130s for 25.0ml of remaining 425.0ml @14:30:12.76 Intake.select :open @14:30:14.93 Intake @15.9psia, vacuum from 10.0 to 13.0psi @14:30:14.95 SS.reconfigure :inhaling 0.47ml/s @14:30:15.00 SS.seek 25ml @14:31:10.76 Stroke completed -- waiting for pressure to equalize ... @14:31:10.77 SS.reconfigure :exhale @14:31:10.82 SS.hold @14:31:35.34 Intake.select :close @14:31:35.50 SSV.dial :exhaust @14:31:36.35 Exhaust.select :open @14:31:36.47 SS.seek 0ml @14:31:45.45 Exhaust.select :close @14:31:45.56 SSV.dial CBV @14:31:45.58
CBV.dial SSV @14:31:46.37 Allowing 130s for 25.0ml of remaining 400.0ml @14:31:46.38 Intake.select :open @14:31:48.55 Intake @15.9psia, vacuum from 10.0 to 13.0psi @14:31:48.57 SS.reconfigure :inhaling 0.47ml/s @14:31:48.62 SS.seek 25ml @14:32:43.98 Stroke completed -- waiting for pressure to equalize ... @14:32:43.99 SS.reconfigure :exhale @14:32:44.04 SS.hold @14:33:10.58 Intake.select :close @14:33:11.10 SSV.dial :exhaust @14:33:11.93 Exhaust.select :open @14:33:12.05 SS.seek 0ml @14:33:20.85 Exhaust.select :close @14:33:20.97 SSV.dial CBV @14:33:21.01
CBV.dial SSV @14:33:21.75 Allowing 130s for 25.0ml of remaining 375.0ml @14:33:21.76 Intake.select :open @14:33:23.93 Intake @16.0psia, vacuum from 10.0 to 13.0psi @14:33:23.95 SS.reconfigure :inhaling 0.47ml/s @14:33:23.99 SS.seek 25ml @14:34:19.51 Stroke completed -- waiting for pressure to equalize ... @14:34:19.52 SS.reconfigure :exhale @14:34:19.57 SS.hold @14:34:48.57 Intake.select :close @14:34:48.72 SSV.dial :exhaust @14:34:49.48 Exhaust.select :open @14:34:49.59 SS.seek 0ml @14:34:58.38 Exhaust.select :close @14:34:58.49 SSV.dial CBV @14:34:58.53
CBV.dial SSV @14:34:59.27 Allowing 130s for 25.0ml of remaining 350.0ml @14:34:59.29 Intake.select :open @14:35:01.45 Intake @16.0psia, vacuum from 10.0 to 13.0psi @14:35:01.46 SS.reconfigure :inhaling 0.47ml/s @14:35:01.51 SS.seek 25ml @14:35:56.99 Stroke completed -- waiting for pressure to equalize ... @14:35:57.00 SS.reconfigure :exhale @14:35:57.05 SS.hold @14:36:29.79 Intake.select :close @14:36:29.95 SSV.dial :exhaust @14:36:30.74 Exhaust.select :open @14:36:30.85 SS.seek 0ml @14:36:39.68 Exhaust.select :close @14:36:39.79 SSV.dial CBV @14:36:39.81
CBV.dial SSV @14:36:40.65 Allowing 130s for 25.0ml of remaining 325.0ml @14:36:40.66 Intake.select :open @14:36:42.83 Intake @16.0psia, vacuum from 10.0 to 13.0psi @14:36:42.85 SS.reconfigure :inhaling 0.47ml/s @14:36:42.89 SS.seek 25ml @14:37:37.29 Vacuum too high -- waiting up to 40 seconds @14:37:37.31 SS.reconfigure :exhale @14:37:37.36 SS.hold @14:37:55.90 Intake @18.4psia, vacuum from 10.0 to 13.0psi @14:37:55.91 SS.reconfigure :inhaling 0.38ml/s @14:37:55.96 SS.seek 25ml @14:37:57.88 Stroke completed -- waiting for pressure to equalize ... @14:37:57.89 SS.reconfigure :exhale @14:37:57.94 SS.hold @14:38:14.26 Intake.select :close @14:38:14.42 SSV.dial :exhaust @14:38:15.18 Exhaust.select :open @14:38:15.29 SS.seek 0ml @14:38:24.46 Exhaust.select :close @14:38:24.57 SSV.dial CBV @14:38:24.59
CBV.dial SSV @14:38:25.38 Allowing 130s for 25.0ml of remaining 300.0ml @14:38:25.39 Intake.select :open @14:38:27.56 Intake @16.0psia, vacuum from 10.0 to 13.0psi @14:38:27.57 SS.reconfigure :inhaling 0.38ml/s @14:38:27.62 SS.seek 25ml @14:39:35.60 Stroke completed -- waiting for pressure to equalize ... @14:39:35.61 SS.reconfigure :exhale @14:39:35.65 SS.hold @14:40:06.73 Intake.select :close @14:40:06.88 SSV.dial :exhaust @14:40:07.64 Exhaust.select :open @14:40:07.76 SS.seek 0ml @14:40:16.61 Exhaust.select :close @14:40:16.72 SSV.dial CBV @14:40:16.75
CBV.dial SSV @14:40:17.53 Allowing 130s for 25.0ml of remaining 275.0ml @14:40:17.55 Intake.select :open @14:40:19.71 Intake @16.0psia, vacuum from 10.0 to 13.0psi @14:40:20.12 SS.reconfigure :inhaling 0.38ml/s @14:40:20.21 SS.seek 25ml @14:41:28.91 Stroke completed -- waiting for pressure to equalize ... @14:41:28.92 SS.reconfigure :exhale @14:41:28.96 SS.hold @14:42:01.70 Intake.select :close @14:42:01.85 SSV.dial :exhaust @14:42:02.62 Exhaust.select :open @14:42:02.73 SS.seek 0ml @14:42:11.97 Exhaust.select :close @14:42:12.08 SSV.dial CBV @14:42:12.10
CBV.dial SSV @14:42:12.91 Allowing 130s for 25.0ml of remaining 250.0ml @14:42:12.92 Intake.select :open @14:42:15.09 Intake @16.0psia, vacuum from 10.0 to 13.0psi @14:42:15.11 SS.reconfigure :inhaling 0.38ml/s @14:42:15.15 SS.seek 25ml @14:43:23.89 Stroke completed -- waiting for pressure to equalize ... @14:43:23.90 SS.reconfigure :exhale @14:43:23.95 SS.hold @14:44:00.80 Intake.select :close @14:44:00.95 SSV.dial :exhaust @14:44:01.72 Exhaust.select :open @14:44:01.83 SS.seek 0ml @14:44:10.86 Exhaust.select :close @14:44:10.96 SSV.dial CBV @14:44:10.99
CBV.dial SSV @14:44:11.78 Allowing 130s for 25.0ml of remaining 225.0ml @14:44:11.79 Intake.select :open @14:44:13.96 Intake @16.0psia, vacuum from 10.0 to 13.0psi @14:44:13.97 SS.reconfigure :inhaling 0.38ml/s @14:44:14.02 SS.seek 25ml @14:45:22.21 Stroke completed -- waiting for pressure to equalize ... @14:45:22.22 SS.reconfigure :exhale @14:45:22.27 SS.hold @14:46:02.45 SS.reconfigure :default @14:46:02.50 SS.hold @14:46:02.57 Intake.select :close @14:46:02.72 ShallowSampler::Clogged in MAIN -- Intake @16psia & Outlet @15psia won't equalize after 800.0ml @14:46:02.73 SSV.dial :exhaust @14:46:03.50 Exhaust.select :open @14:46:03.61 SS.reconfigure :exhale @14:46:03.65 SS.seek 0ml @14:46:12.42 Exhaust.select :close @14:46:12.52 Sampled 800.0ml @14:46:12.54 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @14:46:12.55 Intake.select :close @14:46:12.61 CSV.dial :waste @14:46:12.78 SSV.dial CBV,avoiding: :exhaust @14:46:12.80 CS.seek 0ml @14:46:12.83
CBV.dial SSV @14:46:12.97 CSV.dial :air @14:46:13.72 CS.seek 10ml @14:46:14.75
CTV.dial :air @14:46:15.51 delay 15 seconds @14:46:30.53 SSV.dial :spit,avoiding: :exhaust @14:46:31.88 CTV.dial CSV,avoiding: :intake @14:46:31.92
SS.seek 0ml @14:46:32.03 SSV.dial CBV,avoiding: :exhaust @14:46:38.48 SSV.dial between CBV and plug @14:46:38.97 CBV.dial between SSV and waste2 @14:46:39.36 CSV.dial between air and CTV @14:46:39.69 CS.seek 8.411ml @14:46:44.14 CTV.dial CSV,avoiding: :air @14:46:44.16
CSV.dial CTV @14:46:44.56 CS.reconfigure :pressurize @14:46:44.61 CS.seek 0ml @14:46:58.96 BubblePoint: Intake @24.2psia, Outlet @0.9psia @14:46:58.97 CBV.dial SSV @14:46:59.31 SS.reconfigure :default @14:46:59.36 SS.seek 10ml @14:47:04.96 delay 15 seconds @14:47:20.10 Evacuated: Intake @23.6psia, Outlet @23.6psia @14:47:20.11 SSV.dial :spit,avoiding: :exhaust @14:47:21.14 SS.reconfigure :exhale @14:47:21.19 SS.seek 0ml @14:47:26.12 SS.reconfigure :fast @14:47:26.17 SS.seek 5ml @14:47:28.92 SSV.dial CBV,avoiding: :exhaust @14:47:28.94
CBV.dial SSV @14:47:30.31 CTV.dial :air,avoiding: :intake @14:47:31.62 CSV.dial CTV @14:47:31.65
SS.seek 10ml @14:47:32.86 CSV.dial :air @14:47:33.31 CS.reconfigure :fast @14:47:33.36 CS.seek 0ml @14:47:34.18
SSV.dial :spit,avoiding: :exhaust @14:47:35.52 SS.reconfigure :exhale @14:47:35.57 SS.seek 0ml @14:47:40.58 SS.reconfigure :fast @14:47:40.63 SS.seek 5ml @14:47:41.66 CS.stop @14:47:43.41 SSV.dial CBV,avoiding: :exhaust @14:47:43.45
CBV.dial SSV @14:47:45.77 SSV.dial :spit,avoiding: :exhaust @14:47:47.09 SS.reconfigure :default @14:47:47.14 SS.seek 0ml @14:47:50.41 SS.stop @14:47:50.46 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @14:47:50.47 Intake.select :close @14:47:50.53 CSV.dial :waste @14:47:50.66 SSV.dial CBV,avoiding: :exhaust @14:47:50.68
CBV.dial SSV @14:47:51.29 CS.seek 0ml @14:47:51.36 CSV.dial :air @14:47:52.04
CTV.dial :air @14:47:52.12 CS.seek 10ml @14:47:52.19
delay 15 seconds @14:48:07.21 SSV.dial :spit,avoiding: :exhaust @14:48:08.53 CTV.dial CSV,avoiding: :intake @14:48:08.56
SS.reconfigure :exhale @14:48:08.67 SS.seek 0ml @14:48:09.06 SSV.dial CBV,avoiding: :exhaust @14:48:16.79 SSV.dial between CBV and plug @14:48:17.65 CBV.dial between SSV and waste2 @14:48:18.11 CSV.dial between air and CTV @14:48:18.44 CS.seek 7.585ml @14:48:24.83 CTV.dial CSV,avoiding: :air @14:48:24.85
CSV.dial CTV @14:48:25.20 CS.reconfigure :pressurize @14:48:25.25 CS.seek 0ml @14:48:38.54 BubblePoint: Intake @24.1psia, Outlet @3.5psia @14:48:38.55 CBV.dial SSV @14:48:38.90 SS.reconfigure :default @14:48:38.95 SS.seek 10ml @14:48:44.63 delay 15 seconds @14:48:59.77 Evacuated: Intake @23.9psia, Outlet @24.0psia @14:48:59.78 SSV.dial :spit,avoiding: :exhaust @14:49:00.81 SS.reconfigure :exhale @14:49:00.86 SS.seek 0ml @14:49:06.21 SS.reconfigure :fast @14:49:06.26 SS.seek 5ml @14:49:08.94 SSV.dial CBV,avoiding: :exhaust @14:49:08.98
CBV.dial SSV @14:49:10.29 CTV.dial :air,avoiding: :intake @14:49:11.63 CSV.dial CTV @14:49:11.69
SS.seek 10ml @14:49:12.78 CSV.dial :air @14:49:13.25 CS.reconfigure :fast @14:49:13.30 CS.seek 0ml @14:49:14.43
SSV.dial :spit,avoiding: :exhaust @14:49:15.75 SS.reconfigure :exhale @14:49:15.80 SS.seek 0ml @14:49:20.07 CS.stop @14:49:20.86
SS.reconfigure :fast @14:49:20.91 SS.seek 5ml @14:49:23.84 SSV.dial CBV,avoiding: :exhaust @14:49:23.86
CBV.dial SSV @14:49:26.26 SSV.dial :spit,avoiding: :exhaust @14:49:27.59 SS.reconfigure :default @14:49:27.64 SS.seek 0ml @14:49:30.75 SS.stop @14:49:30.79 Lysing: Adding 2.0ml AqMeOH @14:49:30.80 CBV.dial between CSV and waste1,avoiding: :DBV @14:49:32.30 CTV.dial CSV @14:49:33.62 CSV.dial CSR @14:49:34.40 Pulling 1ml of CSR.AqMeOH @14:49:34.41 CSR.select :AqMeOH @14:49:34.89 CS.reconfigure :default @14:49:35.00 CS.seek 1ml @14:49:38.41 delay 5 seconds @14:49:43.43 CSR.select :air @14:49:43.60 CS.seek 6.001ml @14:49:57.26 delay 2 seconds @14:49:59.27 CSV.dial :waste2 @14:49:59.71 CS.reconfigure :fast @14:49:59.76 CS.seek 0ml @14:50:14.16 CSV.dial CSR @14:50:14.61 Pulling 2ml of CSR.AqMeOH @14:50:14.62 CSR.select :AqMeOH @14:50:14.74 CS.reconfigure :default @14:50:14.79 CS.seek 2ml @14:50:20.94 delay 5 seconds @14:50:25.96 CSR.select :air @14:50:26.13 CS.seek 5ml @14:50:34.69 delay 2 seconds @14:50:36.71 CSV.dial CTV @14:50:37.17 CS.seek 1ml @14:50:48.49 Before lysing Collection Puck Heater at 12.9C/31.2psia @14:50:48.51 Heating Puck.from 4, :sh1 in Collection Clamp to 70C for 10:00 @14:50:48.53 CH.seek 70C,8:00 @14:53:03.38 CH.hold 10:00,70C @14:53:03.57 Begun lysing Collection Puck Heater at 68.1C/38.6psia @15:03:03.59 Ended lysing Collection Puck Heater at 70C/37.6psia @15:03:03.61 CS.seek 0ml @15:03:07.00 CTV.dial between spare and air,avoiding: :intake @15:03:08.03 CSV.dial :air @15:03:08.47 CS.seek 5ml @15:03:22.09 CSV.dial CTV @15:03:22.54 delay 2 seconds @15:03:24.56 CSV.dial :air @15:03:25.01 delay 2 seconds @15:03:27.03 CSV.dial CTV @15:03:27.49 delay 2 seconds @15:03:29.50 CSV.dial :air @15:03:29.95 delay 2 seconds @15:03:31.97 CS.seek 0ml @15:03:45.61 Puck @82.4psia after final lysis pressurization @15:03:45.62 Recovering Lysate into Collection syringe @15:03:45.63 CSV.dial CBV @15:03:46.73 CBV.dial CSV @15:03:47.12 CS.seek 5ml @15:04:00.68 delay 5 seconds @15:04:05.70 CTV.dial :air @15:04:06.23 CS.seek 7ml @15:04:12.12 delay 5 seconds @15:04:17.14 CSV.dial :air2 @15:04:17.59 CS.seek 10ml @15:04:26.12 CSV.dial between air and CTV,avoiding: CBV @15:04:27.28 Lysing Complete @15:04:27.35 Storing sh1 puck in Collection Clamp to tube 3 @15:04:27.52 SC.reconfigure :tight @15:04:27.57 SC.seek 3 @15:04:31.18 SC.configure :limp @15:04:31.40 Elbow.reconfigure :tight @15:04:31.45 Elbow.seek 3 @15:04:33.22 Forearm.reconfigure :tight @15:04:33.27 Forearm.seek 3 + 450 counts @15:04:34.32 SE.reconfigure :pick @15:04:34.38 SE.seek :beamBreak @15:04:36.16 SE.configure :default @15:04:36.33 Elbow.reconfigure :limp @15:04:36.38 Forearm.reconfigure :loose @15:04:36.43 Forearm.seek 3 @15:04:36.69 Elbow.hold @15:04:36.76 Elbow.reconfigure :tight @15:04:36.86 SE.seek 21.11 Pucks below Top Plate @15:04:37.49 Forearm.force -20 @15:04:37.53 Forearm.seek 3 + 287 counts @15:04:37.81 Forearm.reconfigure :tight @15:04:37.86 Forearm.seek 3 + 450 counts @15:04:38.06 SE.seek 22.11 Pucks below Top Plate @15:04:38.68 Forearm.reconfigure :loose @15:04:39.12 Forearm.seek :retract @15:04:40.12 Elbow.seek CC @15:04:41.81 Elbow.reconfigure :loose @15:04:41.91 Forearm.seek CC @15:04:42.93 Hand.close @15:04:43.59 CC.seek :open @15:04:48.32 Forearm.seek :retract @15:04:49.31 Elbow.reconfigure :tight @15:04:49.36 Elbow.seek 3 @15:04:51.11 Elbow.reconfigure :limp @15:04:51.16 Forearm.seek 3 @15:04:52.18 Elbow.hold @15:04:52.25 Elbow.reconfigure :tight @15:04:52.29 Hand.open @15:04:53.08 Forearm.force -20 @15:04:53.13 Forearm.seek 3 + 317 counts @15:04:53.42 Forearm.reconfigure :tight @15:04:53.47 Forearm.seek 3 + 450 counts @15:04:53.75 SE.seek :down @15:04:54.88
Forearm.reconfigure :loose @15:04:54.93 Forearm.seek :retract @15:04:54.99 SE.stop @15:04:55.90
Tranferring DA @15:04:55.91 Beginning samplePassC @15:04:55.93 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @15:04:56.11 Elbow.seek FlushPuck::Garage @15:04:57.99 Elbow.reconfigure :loose @15:04:58.09 Forearm.seek :garage @15:04:59.05 Hand.close @15:05:00.27 Forearm.seek :retract @15:05:01.23 Elbow.reconfigure :tight @15:05:01.28 Elbow.seek CC @15:05:04.19 Elbow.reconfigure :loose @15:05:04.31 Forearm.reconfigure :tight @15:05:04.35 Forearm.seek CC @15:05:05.75 CC.seek :closed @15:05:10.29 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.29 @15:05:10.37 Hand.open @15:05:11.02 Forearm.reconfigure :loose @15:05:11.07 Forearm.seek :retract @15:05:12.05 PSV.dial PSR @15:05:13.45 Pulling 1.5ml of PSR.flush @15:05:13.46 PSR.select :flush @15:05:13.58 PS.seek 1.5ml @15:05:29.78 delay 5 seconds @15:05:34.80 PSV.dial :waste1 @15:05:35.27 PS.seek 0ml @15:05:51.65 PSV.dial PSR @15:05:52.12 PSR.select :air @15:05:52.24 PS.seek 2.5ml @15:06:18.53 PRV.dial CBV,avoiding: :ab2 @15:06:18.55
CBV.dial PRV @15:06:20.32 PSV.dial :mixloop @15:06:21.37 CTV.dial :air,avoiding: :intake @15:06:21.57 PTV.dial :PRVmixing @15:06:22.91 PS.seek 0ml @15:06:49.78 PSV.dial :air @15:06:50.32 PS.seek 2.5ml @15:07:16.68 PSV.dial :mixloop @15:07:17.17 PS.seek 0ml @15:07:43.53 PSV.dial :air @15:07:44.00 PS.seek 2.5ml @15:08:10.43 PSV.dial :mixloop @15:08:10.91 PS.seek 0ml @15:08:37.18 Garaging FlushPuck in Collection Clamp @15:08:37.26 Elbow.reconfigure :tight @15:08:37.30 Elbow.seek CC @15:08:37.61 Elbow.reconfigure :loose @15:08:37.71 Forearm.seek CC @15:08:38.77 Hand.close @15:08:39.34 CC.seek :open @15:08:43.95 Forearm.seek :retract @15:08:44.94 Elbow.reconfigure :tight @15:08:44.99 Elbow.seek :garage @15:08:47.95 Elbow.reconfigure :loose @15:08:48.06 Forearm.reconfigure :tight @15:08:48.11 Forearm.seek :garage @15:08:49.16 Hand.open @15:08:49.89 Forearm.reconfigure :loose @15:08:49.94 Forearm.seek :retract @15:08:50.93 PSV.dial :mixloop @15:08:50.99 PTV.dial :PRVmixing @15:08:51.08 PRV.dial CBV @15:08:51.12
CBV.dial PRV @15:08:51.28 CTV.dial :air,avoiding: :intake @15:08:51.30 Loading lyfil puck from tube 4 into Collection Clamp @15:08:51.53 SC.reconfigure :tight @15:08:51.57 SC.seek 4 @15:08:55.14 SC.configure :limp @15:08:55.76 Elbow.reconfigure :tight @15:08:55.84 Elbow.seek 4 @15:08:57.81 Forearm.reconfigure :tight @15:08:57.86 Forearm.seek 4 + 450 counts @15:08:58.85 SE.reconfigure :pick @15:08:58.91 SE.seek :beamBreak @15:09:01.43 SE.configure :default @15:09:01.59 Elbow.seek 4 @15:09:01.78 Forearm.seek 4 + 300 counts @15:09:01.99 Forearm.reconfigure :loose @15:09:02.04 Hand.close @15:09:02.72 SE.seek :down @15:09:02.93
Forearm.seek :retract @15:09:03.87 Elbow.seek CC @15:09:04.90 SE.stop @15:09:05.47
Elbow.reconfigure :loose @15:09:05.57 Forearm.reconfigure :tight @15:09:05.62 Forearm.seek CC @15:09:06.85 CC.seek :closed @15:09:11.37 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.20 @15:09:11.38 Hand.open @15:09:12.02 Forearm.reconfigure :loose @15:09:12.45 Forearm.seek :retract @15:09:13.48 CTV.dial CSV,avoiding: :intake @15:09:14.79 CSV.dial CTV @15:09:15.23 PS.seek 2.5ml @15:09:41.68 CS.seek 4.5ml @15:09:56.61 delay 8 seconds @15:10:04.64 PSV.dial :air @15:10:05.10 delay 2 seconds @15:10:07.12 PSV.dial :waste2 @15:10:07.87 PS.seek 0ml @15:10:34.15 CBV.dial between PRV and waste1 @15:10:34.47 CSV.dial :air @15:10:34.93 CS.seek 10ml @15:10:49.86 CSV.dial CTV @15:10:50.32 CS.seek 0ml @15:11:17.36 CBV.dial :waste1 @15:11:17.68 delay 5 seconds @15:11:22.70 CTV.dial :air,avoiding: :intake @15:11:24.11 Storing lyfil puck in Collection Clamp to tube 3 @15:11:24.29 SC.reconfigure :tight @15:11:24.33 SC.seek 3 @15:11:27.90 SC.configure :limp @15:11:28.12 Elbow.reconfigure :tight @15:11:28.18 Elbow.seek 3 @15:11:29.89 Forearm.reconfigure :tight @15:11:29.94 Forearm.seek 3 + 450 counts @15:11:30.94 SE.reconfigure :pick @15:11:31.00 SE.seek :beamBreak @15:11:32.64 SE.configure :default @15:11:32.81 Elbow.reconfigure :limp @15:11:32.86 Forearm.reconfigure :loose @15:11:32.90 Forearm.seek 3 @15:11:33.17 Elbow.hold @15:11:33.24 Elbow.reconfigure :tight @15:11:33.78 SE.seek 22.13 Pucks below Top Plate @15:11:34.20 Forearm.force -20 @15:11:34.24 Forearm.seek 3 + 311 counts @15:11:34.53 Forearm.reconfigure :tight @15:11:34.58 Forearm.seek 3 + 450 counts @15:11:34.77 SE.seek 23.13 Pucks below Top Plate @15:11:35.45 Forearm.reconfigure :loose @15:11:35.50 Forearm.seek :retract @15:11:36.43 Elbow.seek CC @15:11:38.12 Elbow.reconfigure :loose @15:11:38.22 Forearm.seek CC @15:11:39.18 Hand.close @15:11:39.83 CC.seek :open @15:11:44.56 Forearm.seek :retract @15:11:45.58 Elbow.reconfigure :tight @15:11:45.63 Elbow.seek 3 @15:11:47.40 Elbow.reconfigure :limp @15:11:47.45 Forearm.seek 3 @15:11:48.50 Elbow.hold @15:11:48.57 Elbow.reconfigure :tight @15:11:48.62 Hand.open @15:11:49.71 Forearm.force -20 @15:11:49.76 Forearm.seek 3 + 313 counts @15:11:50.06 Forearm.reconfigure :tight @15:11:50.11 Forearm.seek 3 + 450 counts @15:11:50.80 SE.seek :down @15:11:51.96
Forearm.reconfigure :loose @15:11:52.03 SE.stop @15:11:52.05
Forearm.seek :retract @15:11:53.02 PSV.dial :air @15:11:53.78 PS.seek 2.5ml @15:12:20.18 delay 1 second @15:12:21.19 PSV.dial :mixloop @15:12:21.65 delay 1 second @15:12:22.66 PSV.dial :air @15:12:23.13 delay 1 second @15:12:24.15 PSV.dial :mixloop @15:12:24.62 delay 1 second @15:12:25.63 PSV.dial :air @15:12:26.10 delay 1 second @15:12:27.12 PS.seek 0ml @15:12:53.49 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @15:12:53.68 Elbow.seek FlushPuck::Garage @15:12:55.50 Elbow.reconfigure :loose @15:12:55.60 Forearm.seek :garage @15:12:56.55 Hand.close @15:12:57.33 Forearm.seek :retract @15:12:58.30 Elbow.reconfigure :tight @15:12:58.35 Elbow.seek PC @15:12:59.59 Elbow.reconfigure :loose @15:12:59.69 Forearm.reconfigure :tight @15:12:59.74 Forearm.seek PC @15:13:00.93 PC.seek :closed @15:13:05.74 Processing Clamp onSpring/offSpring for 14/26 samples, on/off torque 1.64 @15:13:05.81 Hand.open @15:13:06.52 Forearm.reconfigure :loose @15:13:06.57 Forearm.seek :retract @15:13:07.50 PRV.dial :air @15:13:07.97 PSV.dial :mixloop @15:13:08.51 PS.seek 0.3ml @15:13:12.39 PSV.dial PSR @15:13:13.46 Pulling 0.4ml of PSR.air @15:13:13.47 PSR.select :air @15:13:13.50 PS.seek 0.7ml @15:13:18.40 delay 3 seconds @15:13:21.41 PSR.select :air @15:13:21.44 PS.seek 2.5ml @15:13:40.61 delay 3 seconds @15:13:43.63 PSV.dial :mixloop @15:13:44.69 PTV.dial :mixing @15:13:45.44 PBV.dial :waste2 @15:13:46.40 PS.seek 0ml @15:14:12.71 Garaging FlushPuck in Processing Clamp @15:14:12.78 Elbow.reconfigure :tight @15:14:12.83 Elbow.seek PC @15:14:12.95 Elbow.reconfigure :loose @15:14:13.05 Forearm.seek PC @15:14:14.00 Hand.close @15:14:14.58 PC.seek :open @15:14:19.29 Forearm.seek :retract @15:14:20.35 Elbow.reconfigure :tight @15:14:20.39 Elbow.seek :garage @15:14:21.79 Elbow.reconfigure :loose @15:14:21.89 Forearm.reconfigure :tight @15:14:21.94 Forearm.seek :garage @15:14:22.98 Hand.open @15:14:23.64 Forearm.reconfigure :loose @15:14:23.69 Forearm.seek :retract @15:14:24.68 Loading da puck from tube 4 into Processing Clamp @15:14:25.33 SC.reconfigure :tight @15:14:25.40 SC.seek 4 @15:14:28.98 SC.configure :limp @15:14:29.20 Elbow.reconfigure :tight @15:14:29.25 Elbow.seek 4 @15:14:31.12 Forearm.reconfigure :tight @15:14:31.17 Forearm.seek 4 + 450 counts @15:14:32.20 SE.reconfigure :pick @15:14:32.26 SE.seek :beamBreak @15:14:34.81 SE.configure :default @15:14:34.97 Elbow.seek 4 @15:14:35.15 Forearm.seek 4 + 300 counts @15:14:35.41 Forearm.reconfigure :loose @15:14:35.45 Hand.close @15:14:36.10 SE.seek :down @15:14:36.29
Forearm.seek :retract @15:14:37.25 Elbow.seek PC @15:14:38.35 SE.stop @15:14:39.82
Elbow.reconfigure :loose @15:14:39.92 Forearm.reconfigure :tight @15:14:39.97 Forearm.seek PC @15:14:41.29 PC.seek :closed @15:14:45.94 Processing Clamp onSpring/offSpring for 16/26 samples, on/off torque 1.64 @15:14:45.95 Hand.open @15:14:47.02 Forearm.reconfigure :loose @15:14:47.13 Forearm.seek :retract @15:14:48.06 Heating Puck.from 4, :da in Processing Clamp to 28C for 2:00:00 @15:14:48.07 PH.seek 28C,5:00 @15:15:32.45 PH.hold 2:00:00,28C @15:15:32.50 delay 1:30 @15:17:02.54 PSR.select :air @15:17:02.56 PSV.dial PSR @15:17:03.61 Pulling 0.5ml of PSR.wash @15:17:03.62 PSR.select :wash @15:17:03.74 PS.seek 0.5ml @15:17:09.70 delay 5 seconds @15:17:14.72 PSR.select :air @15:17:14.84 PS.seek 2ml @15:17:30.98 PTV.dial :puck @15:17:32.17 PSV.dial PTV @15:17:32.94 PBV.dial :waste1 @15:17:34.33 PS.seek 1.5ml @15:17:40.24 delay 3 seconds @15:17:43.25 PBV.dial between PSV and waste1 @15:17:43.62 PS.seek 0ml @15:17:59.92 delay 5:00 @15:22:59.94 recoverPSRreagent08 @15:22:59.95 PTV.dial :puck @15:23:00.03 PBV.dial :waste1 @15:23:00.40 delay 30 seconds @15:23:30.43 PBV.dial between PSV and waste1 @15:23:30.81 PSR.select :air @15:23:30.82 PSV.dial PSR @15:23:31.59 PS.reconfigure :fast @15:23:31.65 PS.seek 2.5ml @15:23:55.60 PSV.dial PTV @15:23:56.50 delay 5 seconds @15:24:01.52 PSV.dial PSR @15:24:02.26 delay 5 seconds @15:24:07.28 PSV.dial PTV @15:24:08.04 delay 5 seconds @15:24:13.06 PSV.dial :air @15:24:13.98 PS.seek 0ml @15:24:37.42 PTV.dial :PRVmixing,via: PRV @15:24:38.74 PSV.dial :mixloop @15:24:39.20 PRV.dial :ab1 @15:24:39.95 PS.reconfigure :slow @15:24:40.00 PS.seek 0.15ml @15:24:43.67 delay 2 seconds @15:24:45.69 CBV.dial PRV @15:24:45.73
PRV.dial CBV @15:24:46.28 PS.seek 0.35ml @15:24:50.92 delay 2 seconds @15:24:52.93 CBV.dial between PRV and PSV @15:24:53.26 PRV.dial :air @15:24:53.72 PS.seek 0.9ml @15:25:05.44 PRV.dial :waste2 @15:25:06.20 PS.seek 0.5ml @15:25:14.92 addPMLReagent08 @15:25:14.93 PSV.dial :air @15:25:15.39 PS.reconfigure :default @15:25:15.44 PS.seek 2ml @15:25:31.66 PSV.dial :PML @15:25:32.13 PTV.dial :mixing @15:25:32.88 PBV.dial :waste1 @15:25:33.32 PS.seek 1.85ml @15:25:35.69 PBV.dial between PSV and waste1 @15:25:36.52 PS.seek 0ml @15:25:56.26 delay 2 seconds @15:25:58.28 Beginning samplePassC @15:25:58.29 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @15:25:58.48 Elbow.reconfigure :tight @15:25:58.53 Elbow.seek FlushPuck::Garage @15:25:59.89 Elbow.reconfigure :loose @15:26:00.00 Forearm.seek :garage @15:26:00.98 Hand.close @15:26:01.70 Forearm.seek :retract @15:26:02.65 Elbow.reconfigure :tight @15:26:02.70 Elbow.seek CC @15:26:05.63 Elbow.reconfigure :loose @15:26:05.73 Forearm.reconfigure :tight @15:26:05.78 Forearm.seek CC @15:26:07.05 CC.seek :closed @15:26:11.58 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.37 @15:26:11.64 Hand.open @15:26:12.26 Forearm.reconfigure :loose @15:26:12.31 Forearm.seek :retract @15:26:13.32 PSV.dial PSR @15:26:14.51 Pulling 1.5ml of PSR.flush @15:26:14.52 PSR.select :flush @15:26:14.64 PS.seek 1.5ml @15:26:30.79 delay 5 seconds @15:26:35.81 PSV.dial :waste1 @15:26:36.29 PS.seek 0ml @15:26:52.49 PSV.dial PSR @15:26:52.96 PSR.select :air @15:26:53.08 PS.seek 2.5ml @15:27:19.48 PRV.dial CBV,avoiding: :ab2 @15:27:19.50
CBV.dial PRV @15:27:21.15 PSV.dial :mixloop @15:27:22.21 CTV.dial :air,avoiding: :intake @15:27:22.29 PTV.dial :PRVmixing @15:27:23.08 PS.seek 0ml @15:27:49.38 PSV.dial :air @15:27:49.97 PS.seek 2.5ml @15:28:16.25 PSV.dial :mixloop @15:28:16.71 PS.seek 0ml @15:28:43.00 PSV.dial :air @15:28:43.48 PS.seek 2.5ml @15:29:10.00 PSV.dial :mixloop @15:29:10.48 PS.seek 0ml @15:29:36.82 Garaging FlushPuck in Collection Clamp @15:29:36.90 Elbow.reconfigure :tight @15:29:36.94 Elbow.seek CC @15:29:37.25 Elbow.reconfigure :loose @15:29:37.35 Forearm.seek CC @15:29:38.36 Hand.close @15:29:38.95 CC.seek :open @15:29:43.59 Forearm.seek :retract @15:29:44.59 Elbow.reconfigure :tight @15:29:44.64 Elbow.seek :garage @15:29:47.52 Elbow.reconfigure :loose @15:29:47.62 Forearm.reconfigure :tight @15:29:47.67 Forearm.seek :garage @15:29:48.73 Hand.open @15:29:49.38 Forearm.reconfigure :loose @15:29:49.43 Forearm.seek :retract @15:29:50.41 delayUntil "15:33:58.27" @15:29:50.44 Whole Cell Archive sampling 1000ml @15:29:50.46 Loading wcr puck from tube 4 into Collection Clamp @15:29:51.39 Elbow.reconfigure :tight @15:29:51.44 Elbow.seek 4 @15:29:53.39 Forearm.reconfigure :tight @15:29:53.45 Forearm.seek 4 + 450 counts @15:29:54.51 SE.reconfigure :pick @15:29:54.57 SE.seek :beamBreak @15:29:57.27 SE.configure :default @15:29:57.44 Elbow.seek 4 @15:29:57.63 Forearm.seek 4 + 300 counts @15:29:57.86 Forearm.reconfigure :loose @15:29:57.92 Hand.close @15:29:58.58 SE.seek :down @15:29:58.79 Forearm.seek :retract @15:29:59.76 Elbow.seek CC @15:30:00.87 SE.stop @15:30:01.39 Elbow.reconfigure :loose @15:30:01.50 Forearm.reconfigure :tight @15:30:01.55 Forearm.seek CC @15:30:02.83 CC.seek :closed @15:30:07.36 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.32 @15:30:07.38 Hand.open @15:30:08.01 Forearm.reconfigure :loose @15:30:08.06 Forearm.seek :retract @15:30:09.09 collectSample 1000ml w/bubble point of 35psi @15:30:09.10 Priming 7ml @15:30:09.11 Intake.select :close @15:30:09.12 Exhaust.select :close @15:30:09.14 SS.hold @15:30:09.35 Calibrating pressure sensors @15:30:09.36 CTV.dial :air @15:30:09.44 SSV.dial :spit,avoiding: :exhaust @15:30:09.46 SS.reconfigure :fast @15:30:09.52 SS.seek 5ml @15:30:12.20 SSV.dial CBV @15:30:12.24 CBV.dial SSV @15:30:13.59 delay 3.8 seconds @15:30:17.53 Sensor limits: Intake max 104.5psia; Outlet max 96.0psia @15:30:17.54 SSV.dial :spit @15:30:18.88 SS.seek 0ml @15:30:21.74 SSV.dial CBV @15:30:23.08 CTV.dial :intake @15:30:23.85 SSV.dial CBV @15:30:23.87 CBV.dial SSV @15:30:24.10 Allowing 40s for 7.0ml of remaining 7.0ml @15:30:24.12 Intake.select :open @15:30:26.29 Intake @14.6psia, vacuum from 9.1 to 12.1psi @15:30:26.31 SS.reconfigure :inhaling 2.20ml/s @15:30:26.36 SS.seek 7ml @15:30:33.10 Stroke completed -- waiting for pressure to equalize ... @15:30:33.11 SS.reconfigure :exhale @15:30:33.16 SS.hold @15:30:51.67 Intake.select :close @15:30:51.83 SSV.dial :exhaust @15:30:52.62 Exhaust.select :open @15:30:52.74 SS.seek 0ml @15:30:56.85 Exhaust.select :close @15:30:56.98 Sampling 1000.0ml @15:30:56.99 Intake.select :close @15:30:57.00 Exhaust.select :close @15:30:57.02 SS.reconfigure :default @15:30:57.06 SS.hold @15:30:57.32 CTV.dial :intake @15:30:57.57 SSV.dial CBV @15:30:57.60 CBV.dial SSV @15:30:58.38 Allowing 130s for 25.0ml of remaining 1000.0ml @15:30:58.40 Intake.select :open @15:31:00.57 Intake @15.4psia, vacuum from 9.9 to 12.9psi @15:31:00.59 SS.reconfigure :inhaling 2.20ml/s @15:31:00.63 SS.seek 25ml @15:31:09.20 Vacuum too high -- waiting up to 40 seconds @15:31:09.22 SS.reconfigure :exhale @15:31:09.27 SS.hold @15:31:19.50 Intake @18.9psia, vacuum from 10.0 to 13.0psi @15:31:19.52 SS.reconfigure :inhaling 1.76ml/s @15:31:19.57 SS.seek 25ml @15:31:21.89 Vacuum too high -- waiting up to 40 seconds @15:31:21.91 SS.reconfigure :exhale @15:31:21.97 SS.hold @15:31:24.70 Intake @19.0psia, vacuum from 10.0 to 13.0psi @15:31:24.72 SS.reconfigure :inhaling 1.41ml/s @15:31:24.77 SS.seek 25ml @15:31:27.71 Vacuum too high -- waiting up to 40 seconds @15:31:27.72 SS.reconfigure :exhale @15:31:27.78 SS.hold @15:31:30.43 Intake @18.9psia, vacuum from 10.0 to 13.0psi @15:31:30.45 SS.reconfigure :inhaling 1.13ml/s @15:31:30.50 SS.seek 25ml @15:31:32.96 Vacuum too high -- waiting up to 40 seconds @15:31:32.97 SS.reconfigure :exhale @15:31:33.03 SS.hold @15:31:35.16 Intake @19.0psia, vacuum from 10.0 to 13.0psi @15:31:35.18 SS.reconfigure :inhaling 0.91ml/s @15:31:35.22 SS.seek 25ml @15:31:38.77 Vacuum too high -- waiting up to 40 seconds @15:31:38.79 SS.reconfigure :exhale @15:31:38.84 SS.hold @15:31:41.42 SS.reconfigure :inhaling 0.73ml/s @15:31:41.47 SS.seek 25ml @15:31:44.98 Stroke completed -- waiting for pressure to equalize ... @15:31:44.99 SS.reconfigure :exhale @15:31:45.05 SS.hold @15:32:09.56 Intake.select :close @15:32:10.17 SSV.dial :exhaust @15:32:10.95 Exhaust.select :open @15:32:11.07 SS.seek 0ml @15:32:20.07 Exhaust.select :close @15:32:20.19 SSV.dial CBV @15:32:20.21 CBV.dial SSV @15:32:20.99 Allowing 130s for 25.0ml of remaining 975.0ml @15:32:21.01 Intake.select :open @15:32:23.18 Intake @15.9psia, vacuum from 10.0 to 13.0psi @15:32:23.20 SS.reconfigure :inhaling 0.73ml/s @15:32:23.25 SS.seek 25ml @15:32:59.27 Stroke completed -- waiting for pressure to equalize ... @15:32:59.29 SS.reconfigure :exhale @15:32:59.34 SS.hold @15:33:30.06 Intake.select :close @15:33:30.22 SSV.dial :exhaust @15:33:30.99 Exhaust.select :open @15:33:31.11 SS.seek 0ml @15:33:39.90 Exhaust.select :close @15:33:40.01 SSV.dial CBV @15:33:40.04 CBV.dial SSV @15:33:40.82 Allowing 130s for 25.0ml of remaining 950.0ml @15:33:40.83 Intake.select :open @15:33:43.01 Intake @16.1psia, vacuum from 10.0 to 13.0psi @15:33:43.03 SS.reconfigure :inhaling 0.73ml/s @15:33:43.08 SS.seek 25ml @15:33:58.28 recoverPRVreagent08 @15:33:58.29 PTV.dial :mixing @15:33:59.05 PBV.dial :waste1 @15:33:59.42 delay 30 seconds @15:34:19.71 Stroke completed -- waiting for pressure to equalize ... @15:34:19.72 SS.reconfigure :exhale @15:34:19.77 SS.hold @15:34:29.50 PBV.dial between PSV and waste1 @15:34:29.90 PSV.dial :air @15:34:30.51 PS.reconfigure :fast @15:34:30.56 PS.seek 2.5ml @15:34:46.36 Intake.select :close @15:34:46.52 SSV.dial :exhaust @15:34:47.31 Exhaust.select :open @15:34:47.43 SS.seek 0ml @15:34:54.59 PSV.dial :mixloop @15:34:55.05 delay 2 seconds @15:34:56.33 Exhaust.select :close @15:34:56.44 SSV.dial CBV @15:34:56.46 CBV.dial SSV @15:34:57.06 PSV.dial :air @15:34:57.27 Allowing 130s for 25.0ml of remaining 925.0ml @15:34:57.29 Intake.select :open @15:34:57.65 delay 2 seconds @15:34:59.46 Intake @16.2psia, vacuum from 10.0 to 13.0psi @15:34:59.48 SS.reconfigure :inhaling 0.73ml/s @15:34:59.53 SS.seek 25ml @15:35:00.03 PSV.dial :mixloop @15:35:00.48 delay 2 seconds @15:35:02.50 PSV.dial :air @15:35:03.10 PS.seek 0ml @15:35:26.62 washCycleDA #1 of 2 @15:35:26.63 PSR.select :air @15:35:26.65 PSV.dial PSR @15:35:28.01 Pulling 0.5ml of PSR.wash @15:35:28.02 PSR.select :wash @15:35:28.14 PS.reconfigure :default @15:35:28.19 PS.seek 0.5ml @15:35:34.22 delay 5 seconds @15:35:35.97 Stroke completed -- waiting for pressure to equalize ... @15:35:35.98 SS.reconfigure :exhale @15:35:36.03 SS.hold @15:35:39.23 PSR.select :air @15:35:39.35 PS.seek 2ml @15:35:55.53 PTV.dial :puck @15:35:56.28 PSV.dial PTV @15:35:57.17 PBV.dial :waste1 @15:35:57.61 PS.seek 1.5ml @15:36:03.04 Intake.select :close @15:36:03.20 SSV.dial :exhaust @15:36:03.59 delay 3 seconds @15:36:03.99 Exhaust.select :open @15:36:04.11 SS.seek 0ml @15:36:06.61 PBV.dial between PSV and waste1 @15:36:07.00 PS.seek 0ml @15:36:13.18 Exhaust.select :close @15:36:13.29 SSV.dial CBV @15:36:13.32 CBV.dial SSV @15:36:14.11 Allowing 130s for 25.0ml of remaining 900.0ml @15:36:14.13 Intake.select :open @15:36:16.30 Intake @16.0psia, vacuum from 10.0 to 13.0psi @15:36:16.32 SS.reconfigure :inhaling 0.73ml/s @15:36:16.37 SS.seek 25ml @15:36:23.34 delay 2:00 @15:36:52.13 Stroke completed -- waiting for pressure to equalize ... @15:36:52.14 SS.reconfigure :exhale @15:36:52.19 SS.hold @15:37:16.70 Intake.select :close @15:37:16.86 SSV.dial :exhaust @15:37:17.63 Exhaust.select :open @15:37:17.75 SS.seek 0ml @15:37:26.53 Exhaust.select :close @15:37:26.65 SSV.dial CBV @15:37:26.68 CBV.dial SSV @15:37:27.44 Allowing 130s for 25.0ml of remaining 875.0ml @15:37:27.45 Intake.select :open @15:37:29.62 Intake @16.1psia, vacuum from 10.0 to 13.0psi @15:37:29.64 SS.reconfigure :inhaling 0.73ml/s @15:37:29.69 SS.seek 25ml @15:38:06.23 Stroke completed -- waiting for pressure to equalize ... @15:38:06.25 SS.reconfigure :exhale @15:38:06.30 SS.hold @15:38:23.36 recoverPSRreagent08 @15:38:23.37 PTV.dial :puck @15:38:23.45 PBV.dial :waste1 @15:38:23.82 delay 30 seconds @15:38:29.26 Intake.select :close @15:38:29.42 SSV.dial :exhaust @15:38:30.21 Exhaust.select :open @15:38:30.33 SS.seek 0ml @15:38:39.35 Exhaust.select :close @15:38:39.47 SSV.dial CBV @15:38:39.50 CBV.dial SSV @15:38:40.28 Allowing 130s for 25.0ml of remaining 850.0ml @15:38:40.30 Intake.select :open @15:38:42.92 Intake @16.1psia, vacuum from 10.0 to 13.0psi @15:38:42.94 SS.reconfigure :inhaling 0.73ml/s @15:38:42.99 SS.seek 25ml @15:38:53.85 PBV.dial between PSV and waste1 @15:38:54.23 PSR.select :air @15:38:54.25 PSV.dial PSR @15:38:55.00 PS.reconfigure :fast @15:38:55.05 PS.seek 2.5ml @15:39:18.76 Stroke completed -- waiting for pressure to equalize ... @15:39:18.78 SS.reconfigure :exhale @15:39:18.83 SS.hold @15:39:19.21 PSV.dial PTV @15:39:20.11 delay 5 seconds @15:39:25.12 PSV.dial PSR @15:39:25.87 delay 5 seconds @15:39:30.88 PSV.dial PTV @15:39:31.65 delay 5 seconds @15:39:36.67 PSV.dial :air @15:39:37.62 PS.seek 0ml @15:39:41.29 Intake.select :close @15:39:41.45 SSV.dial :exhaust @15:39:42.22 Exhaust.select :open @15:39:42.34 SS.seek 0ml @15:39:51.37 Exhaust.select :close @15:39:51.48 SSV.dial CBV @15:39:51.52 CBV.dial SSV @15:39:52.30 Allowing 130s for 25.0ml of remaining 825.0ml @15:39:52.31 Intake.select :open @15:39:54.48 Intake @16.1psia, vacuum from 10.0 to 13.0psi @15:39:54.50 SS.reconfigure :inhaling 0.73ml/s @15:39:54.55 SS.seek 25ml @15:40:01.31 washCycleDA #2 of 2 @15:40:01.32 PSR.select :air @15:40:01.34 PSV.dial PSR @15:40:02.69 Pulling 0.5ml of PSR.wash @15:40:02.71 PSR.select :wash @15:40:02.82 PS.reconfigure :default @15:40:02.87 PS.seek 0.5ml @15:40:08.87 delay 5 seconds @15:40:13.89 PSR.select :air @15:40:14.01 PS.seek 2ml @15:40:23.32 Vacuum too high -- waiting up to 40 seconds @15:40:23.34 SS.reconfigure :exhale @15:40:23.39 SS.hold @15:40:30.21 PTV.dial :puck @15:40:30.22 PSV.dial PTV @15:40:31.12 PBV.dial :waste1 @15:40:31.55 PS.seek 1.5ml @15:40:32.56 Intake @17.4psia, vacuum from 10.0 to 13.0psi @15:40:32.58 SS.reconfigure :inhaling 0.59ml/s @15:40:32.63 SS.seek 25ml @15:40:37.49 delay 3 seconds @15:40:40.51 PBV.dial between PSV and waste1 @15:40:40.89 PS.seek 0ml @15:40:41.67 Stroke completed -- waiting for pressure to equalize ... @15:40:41.68 SS.reconfigure :exhale @15:40:41.73 SS.hold @15:40:56.11 Intake.select :close @15:40:56.27 SSV.dial :exhaust @15:40:57.05 Exhaust.select :open @15:40:57.16 SS.seek 0ml @15:40:57.26 delay 2:00 @15:41:06.01 Exhaust.select :close @15:41:06.12 SSV.dial CBV @15:41:06.15 CBV.dial SSV @15:41:06.95 Allowing 130s for 25.0ml of remaining 800.0ml @15:41:06.97 Intake.select :open @15:41:09.13 Intake @16.0psia, vacuum from 10.0 to 13.0psi @15:41:09.15 SS.reconfigure :inhaling 0.59ml/s @15:41:09.20 SS.seek 25ml @15:41:54.04 Stroke completed -- waiting for pressure to equalize ... @15:41:54.06 SS.reconfigure :exhale @15:41:54.10 SS.hold @15:42:14.56 Intake.select :close @15:42:14.72 SSV.dial :exhaust @15:42:15.48 Exhaust.select :open @15:42:15.60 SS.seek 0ml @15:42:24.45 Exhaust.select :close @15:42:24.56 SSV.dial CBV @15:42:24.60 CBV.dial SSV @15:42:25.36 Allowing 130s for 25.0ml of remaining 775.0ml @15:42:25.37 Intake.select :open @15:42:27.54 Intake @16.0psia, vacuum from 10.0 to 13.0psi @15:42:27.56 SS.reconfigure :inhaling 0.59ml/s @15:42:27.61 SS.seek 25ml @15:42:57.30 recoverPSRreagent08 @15:42:57.31 PTV.dial :puck @15:42:57.32 PBV.dial :waste1 @15:42:57.69 delay 30 seconds @15:43:12.34 Stroke completed -- waiting for pressure to equalize ... @15:43:12.35 SS.reconfigure :exhale @15:43:12.40 SS.hold @15:43:27.71 PBV.dial between PSV and waste1 @15:43:28.08 PSR.select :air @15:43:28.10 PSV.dial PSR @15:43:28.85 PS.reconfigure :fast @15:43:28.90 PS.seek 2.5ml @15:43:32.77 Intake.select :close @15:43:32.94 SSV.dial :exhaust @15:43:33.72 Exhaust.select :open @15:43:33.84 SS.seek 0ml @15:43:42.84 Exhaust.select :close @15:43:42.95 SSV.dial CBV @15:43:42.97 CBV.dial SSV @15:43:43.78 Allowing 130s for 25.0ml of remaining 750.0ml @15:43:43.79 Intake.select :open @15:43:45.96 Intake @15.9psia, vacuum from 10.0 to 13.0psi @15:43:45.98 SS.reconfigure :inhaling 0.59ml/s @15:43:46.03 SS.seek 25ml @15:43:52.99 PSV.dial PTV @15:43:53.75 delay 5 seconds @15:43:58.78 PSV.dial PSR @15:43:59.55 delay 5 seconds @15:44:04.57 PSV.dial PTV @15:44:05.35 delay 5 seconds @15:44:10.37 PSV.dial :air @15:44:11.13 PS.seek 0ml @15:44:30.41 Stroke completed -- waiting for pressure to equalize ... @15:44:30.43 SS.reconfigure :exhale @15:44:30.48 SS.hold @15:44:34.67 getPMLReagent08 @15:44:34.69 PRV.dial :air @15:44:35.13 delay 5 seconds @15:44:40.14 PTV.dial :PRVmixing @15:44:41.49 delay 5 seconds @15:44:46.50 PRV.dial :ab2 @15:44:46.97 PTV.dial :PRVmixing @15:44:47.10 PSV.dial :mixloop @15:44:47.69 PS.reconfigure :default @15:44:47.74 PS.seek 0.3ml @15:44:51.59 delay 2 seconds @15:44:53.60 PRV.dial :air @15:44:54.61 PS.seek 0.55ml @15:44:55.46 Intake.select :close @15:44:55.62 SSV.dial :exhaust @15:44:56.38 Exhaust.select :open @15:44:56.50 SS.seek 0ml @15:44:57.93 addPMLReagent08 @15:44:57.94 PSV.dial :air @15:44:58.41 PS.seek 2ml @15:45:05.52 Exhaust.select :close @15:45:05.64 SSV.dial CBV @15:45:05.66 CBV.dial SSV @15:45:06.45 Allowing 130s for 25.0ml of remaining 725.0ml @15:45:06.46 Intake.select :open @15:45:08.63 Intake @15.9psia, vacuum from 10.0 to 13.0psi @15:45:08.65 SS.reconfigure :inhaling 0.59ml/s @15:45:08.70 SS.seek 25ml @15:45:14.02 PSV.dial :PML @15:45:14.50 PTV.dial :mixing @15:45:15.27 PBV.dial :waste1 @15:45:15.71 PS.seek 1.85ml @15:45:18.14 PBV.dial between PSV and waste1 @15:45:18.52 PS.seek 0ml @15:45:38.27 delay 2 seconds @15:45:40.29 delay 8:00 @15:45:47.69 Vacuum too high -- waiting up to 40 seconds @15:45:47.71 SS.reconfigure :exhale @15:45:47.76 SS.hold @15:45:59.09 Intake @16.8psia, vacuum from 10.0 to 13.0psi @15:45:59.11 SS.reconfigure :inhaling 0.47ml/s @15:45:59.15 SS.seek 25ml @15:46:06.32 Stroke completed -- waiting for pressure to equalize ... @15:46:06.33 SS.reconfigure :exhale @15:46:06.38 SS.hold @15:46:20.84 Intake.select :close @15:46:21.00 SSV.dial :exhaust @15:46:21.79 Exhaust.select :open @15:46:21.91 SS.seek 0ml @15:46:30.72 Exhaust.select :close @15:46:30.83 SSV.dial CBV @15:46:30.88 CBV.dial SSV @15:46:31.62 Allowing 130s for 25.0ml of remaining 700.0ml @15:46:31.63 Intake.select :open @15:46:33.80 Intake @15.9psia, vacuum from 10.0 to 13.0psi @15:46:33.82 SS.reconfigure :inhaling 0.47ml/s @15:46:33.87 SS.seek 25ml @15:47:29.36 Stroke completed -- waiting for pressure to equalize ... @15:47:29.38 SS.reconfigure :exhale @15:47:29.42 SS.hold @15:47:52.37 Intake.select :close @15:47:52.53 SSV.dial :exhaust @15:47:53.30 Exhaust.select :open @15:47:53.42 SS.seek 0ml @15:48:02.19 Exhaust.select :close @15:48:02.31 SSV.dial CBV @15:48:02.33 CBV.dial SSV @15:48:03.13 Allowing 130s for 25.0ml of remaining 675.0ml @15:48:03.14 Intake.select :open @15:48:05.31 Intake @15.9psia, vacuum from 10.0 to 13.0psi @15:48:05.33 SS.reconfigure :inhaling 0.47ml/s @15:48:05.38 SS.seek 25ml @15:49:00.85 Stroke completed -- waiting for pressure to equalize ... @15:49:00.86 SS.reconfigure :exhale @15:49:00.91 SS.hold @15:49:27.52 Intake.select :close @15:49:27.68 SSV.dial :exhaust @15:49:28.45 Exhaust.select :open @15:49:28.57 SS.seek 0ml @15:49:37.58 Exhaust.select :close @15:49:37.70 SSV.dial CBV @15:49:37.72 CBV.dial SSV @15:49:38.52 Allowing 130s for 25.0ml of remaining 650.0ml @15:49:38.53 Intake.select :open @15:49:40.70 Intake @15.7psia, vacuum from 10.0 to 13.0psi @15:49:40.72 SS.reconfigure :inhaling 0.47ml/s @15:49:40.77 SS.seek 25ml @15:50:35.86 Stroke completed -- waiting for pressure to equalize ... @15:50:35.87 SS.reconfigure :exhale @15:50:35.92 SS.hold @15:51:06.75 Intake.select :close @15:51:06.91 SSV.dial :exhaust @15:51:07.69 Exhaust.select :open @15:51:07.81 SS.seek 0ml @15:51:16.64 Exhaust.select :close @15:51:16.80 SSV.dial CBV @15:51:16.82 CBV.dial SSV @15:51:17.61 Allowing 130s for 25.0ml of remaining 625.0ml @15:51:17.62 Intake.select :open @15:51:20.25 Intake @15.8psia, vacuum from 10.0 to 13.0psi @15:51:20.27 SS.reconfigure :inhaling 0.47ml/s @15:51:20.32 SS.seek 25ml @15:52:15.84 Stroke completed -- waiting for pressure to equalize ... @15:52:15.86 SS.reconfigure :exhale @15:52:15.91 SS.hold @15:52:50.66 Intake.select :close @15:52:50.82 SSV.dial :exhaust @15:52:51.59 Exhaust.select :open @15:52:51.71 SS.seek 0ml @15:53:00.83 Exhaust.select :close @15:53:00.94 SSV.dial CBV @15:53:00.96 CBV.dial SSV @15:53:01.76 Allowing 130s for 25.0ml of remaining 600.0ml @15:53:01.78 Intake.select :open @15:53:03.95 Intake @15.8psia, vacuum from 10.0 to 13.0psi @15:53:03.97 SS.reconfigure :inhaling 0.47ml/s @15:53:04.02 SS.seek 25ml @15:53:40.31 recoverPRVreagent08 @15:53:40.32 PTV.dial :mixing @15:53:40.40 PBV.dial :waste1 @15:53:40.79 delay 30 seconds @15:53:59.01 Stroke completed -- waiting for pressure to equalize ... @15:53:59.03 SS.reconfigure :exhale @15:53:59.07 SS.hold @15:54:10.81 PBV.dial between PSV and waste1 @15:54:11.18 PSV.dial :air @15:54:11.65 PS.reconfigure :fast @15:54:11.70 PS.seek 2.5ml @15:54:35.85 PSV.dial :mixloop @15:54:36.32 delay 2 seconds @15:54:38.34 PSV.dial :air @15:54:38.80 delay 2 seconds @15:54:39.26 SS.reconfigure :default @15:54:39.31 SS.hold @15:54:39.39 Intake.select :close @15:54:39.55 ShallowSampler::Clogged in WCR -- Intake @16psia & Outlet @14psia won't equalize after 425.0ml @15:54:39.56 SSV.dial :exhaust @15:54:40.33 Exhaust.select :open @15:54:40.44 SS.reconfigure :exhale @15:54:40.96 SS.seek 0ml @15:54:41.01 PSV.dial :mixloop @15:54:41.48 delay 2 seconds @15:54:43.49 PSV.dial :air @15:54:43.97 PS.seek 0ml @15:54:49.73 Exhaust.select :close @15:54:49.85 Sampled 425.0ml @15:54:49.87 Drying puck before fixing for 5:00 @15:54:49.88 CBV.dial :waste2b @15:54:50.65 delay 1:00 @15:55:07.66 washCycleDA #1 of 3 @15:55:07.68 PSR.select :air @15:55:07.69 PSV.dial PSR @15:55:09.04 Pulling 0.5ml of PSR.wash @15:55:09.05 PSR.select :wash @15:55:09.17 PS.reconfigure :default @15:55:09.22 PS.seek 0.5ml @15:55:15.10 delay 5 seconds @15:55:20.12 PSR.select :air @15:55:20.24 PS.seek 2ml @15:55:36.35 PTV.dial :puck @15:55:37.10 PSV.dial PTV @15:55:37.85 PBV.dial :waste1 @15:55:38.28 PS.seek 1.5ml @15:55:44.22 delay 3 seconds @15:55:47.24 PBV.dial between PSV and waste1 @15:55:47.62 PS.seek 0ml @15:55:50.67 CSV.dial :air @15:55:51.12 CS.reconfigure :fast @15:55:51.18 CS.seek 10ml @15:56:03.76 delay 2:00 @15:56:15.82 Pumping wcr puck from tube 4 up to 35psi... @15:56:15.85 CBV.dial SSV @15:56:17.12 CBV.dial between CSV and waste2b @15:56:18.10 CSV.dial between air and CTV @15:56:18.42 CS.seek 7.316ml @15:56:25.39 CTV.dial CSV,avoiding: :air @15:56:25.41 CSV.dial CTV @15:56:26.25 CS.reconfigure :pressurize @15:56:26.30 CS.seek 0ml @15:56:40.06 CBV.dial :waste2b @15:56:40.40 CS.reconfigure :default @15:56:40.45 CS.seek 0ml @15:56:46.60 delay 1:00 @15:57:46.63 CTV.dial :air,avoiding: :intake @15:57:47.95 Fix #1 of 2 (with RNAlater) for 2:30 @15:57:47.97 CSV.dial CSR @15:57:48.40 Pulling 2ml of CSR.RNAlater @15:57:48.41 CSR.select :RNAlater @15:57:48.98 CS.seek 2ml @15:57:54.94 delay 5 seconds @15:57:59.97 CSR.select :air @15:58:00.09 CS.reconfigure :fast @15:58:00.14 CS.seek 10ml @15:58:03.78 recoverPSRreagent08 @15:58:03.79 PTV.dial :puck @15:58:03.86 PBV.dial :waste1 @15:58:04.24 delay 30 seconds @15:58:20.07 Pumping wcr puck from tube 4 up to 35psi... @15:58:20.09 CBV.dial SSV @15:58:21.36 CBV.dial between CSV and waste2b @15:58:22.33 CSV.dial between air and CTV @15:58:23.06 CS.seek 7.232ml @15:58:30.26 CTV.dial CSV,avoiding: :air @15:58:30.28 CSV.dial CTV @15:58:31.67 CS.reconfigure :pressurize @15:58:31.72 CS.seek 0ml @15:58:34.26 PBV.dial between PSV and waste1 @15:58:34.63 PSR.select :air @15:58:34.65 PSV.dial PSR @15:58:35.42 PS.reconfigure :fast @15:58:35.47 PS.seek 2.5ml @15:58:50.09 delay 2:30 @15:58:59.56 PSV.dial PTV @15:59:00.45 delay 5 seconds @15:59:05.48 PSV.dial PSR @15:59:06.22 delay 5 seconds @15:59:11.24 PSV.dial PTV @15:59:12.00 delay 5 seconds @15:59:17.03 PSV.dial :air @15:59:17.80 PS.seek 0ml @15:59:41.16 washCycleDA #2 of 3 @15:59:41.17 PSR.select :air @15:59:41.19 PSV.dial PSR @15:59:42.53 Pulling 0.5ml of PSR.wash @15:59:42.54 PSR.select :wash @15:59:42.66 PS.reconfigure :default @15:59:42.71 PS.seek 0.5ml @15:59:48.66 delay 5 seconds @15:59:53.68 PSR.select :air @15:59:53.80 PS.seek 2ml @16:00:09.93 PTV.dial :puck @16:00:10.01 PSV.dial PTV @16:00:10.76 PBV.dial :waste1 @16:00:11.20 PS.seek 1.5ml @16:00:17.15 delay 3 seconds @16:00:20.17 PBV.dial between PSV and waste1 @16:00:20.55 PS.seek 0ml @16:00:37.12 delay 2:00 @16:01:20.11 CBV.dial :waste2b @16:01:20.44 CS.reconfigure :default @16:01:20.50 CS.seek 0ml @16:01:21.85 delay 1:00 @16:02:21.87 CTV.dial :air,avoiding: :intake @16:02:23.21 Fix #2 of 2 (with RNAlater) for 2:30 @16:02:23.23 CSV.dial CSR @16:02:23.66 Pulling 2ml of CSR.RNAlater @16:02:23.67 CSR.select :RNAlater @16:02:23.79 CS.seek 2ml @16:02:29.78 delay 5 seconds @16:02:34.80 CSR.select :air @16:02:34.92 CS.reconfigure :fast @16:02:34.98 CS.seek 10ml @16:02:37.14 recoverPSRreagent08 @16:02:37.15 PTV.dial :puck @16:02:37.23 PBV.dial :waste1 @16:02:37.61 delay 30 seconds @16:02:54.72 Pumping wcr puck from tube 4 up to 35psi... @16:02:54.74 CBV.dial SSV @16:02:56.01 CBV.dial between CSV and waste2b @16:02:56.98 CSV.dial between air and CTV @16:02:57.59 CS.seek 7.195ml @16:03:04.88 CTV.dial CSV,avoiding: :air @16:03:04.90 CSV.dial CTV @16:03:06.27 CS.reconfigure :pressurize @16:03:06.32 CS.seek 0ml @16:03:07.62 PBV.dial between PSV and waste1 @16:03:08.00 PSR.select :air @16:03:08.01 PSV.dial PSR @16:03:08.78 PS.reconfigure :fast @16:03:08.83 PS.seek 2.5ml @16:03:22.06 delay 2:30 @16:03:32.78 PSV.dial PTV @16:03:33.54 delay 5 seconds @16:03:38.56 PSV.dial PSR @16:03:39.32 delay 5 seconds @16:03:44.35 PSV.dial PTV @16:03:45.10 delay 5 seconds @16:03:50.13 PSV.dial :air @16:03:50.89 PS.seek 0ml @16:04:14.34 washCycleDA #3 of 3 @16:04:14.35 PSR.select :air @16:04:14.37 PSV.dial PSR @16:04:15.71 Pulling 0.5ml of PSR.wash @16:04:15.72 PSR.select :wash @16:04:15.84 PS.reconfigure :default @16:04:15.89 PS.seek 0.5ml @16:04:21.81 delay 5 seconds @16:04:26.83 PSR.select :air @16:04:26.95 PS.seek 2ml @16:04:43.62 PTV.dial :puck @16:04:43.70 PSV.dial PTV @16:04:44.45 PBV.dial :waste1 @16:04:44.88 PS.seek 1.5ml @16:04:50.87 delay 3 seconds @16:04:53.89 PBV.dial between PSV and waste1 @16:04:54.25 PS.seek 0ml @16:05:10.37 delay 2:00 @16:05:52.08 CBV.dial :waste2b @16:05:52.41 CS.reconfigure :default @16:05:52.47 CS.seek 0ml @16:05:56.34 delay 1:00 @16:06:56.37 CTV.dial :air,avoiding: :intake @16:07:10.39 recoverPSRreagent08 @16:07:10.40 PTV.dial :puck @16:07:10.47 PBV.dial :waste1 @16:07:10.85 delay 30 seconds @16:07:40.88 PBV.dial between PSV and waste1 @16:07:41.25 PSR.select :air @16:07:41.28 PSV.dial PSR @16:07:42.04 PS.reconfigure :fast @16:07:42.09 PS.seek 2.5ml @16:08:06.36 PSV.dial PTV @16:08:07.13 delay 5 seconds @16:08:12.15 PSV.dial PSR @16:08:12.91 delay 5 seconds @16:08:17.93 PSV.dial PTV @16:08:18.69 delay 5 seconds @16:08:23.72 PSV.dial :air @16:08:24.50 PS.seek 0ml @16:08:47.96 Gate.power :camera,:ON @16:08:47.97 Mixing DA Substrates @16:08:47.98 PSR.select :air @16:08:48.00 PSV.dial PSR @16:08:49.40 Pulling 0.25ml of PSR.sub1 @16:08:49.43 PSR.select :sub1 @16:08:49.62 PS.reconfigure :default @16:08:49.75 PS.seek 0.25ml @16:08:53.25 delay 5 seconds @16:08:58.27 Pulling 0.25ml of PSR.sub2 @16:08:58.29 PSR.select :sub2 @16:08:58.40 PS.seek 0.5ml @16:09:01.74 delay 5 seconds @16:09:06.76 PSR.select :air @16:09:06.87 PS.seek 2ml @16:09:23.11 PTV.dial :puck @16:09:23.19 PSV.dial PTV @16:09:23.96 PBV.dial :waste1 @16:09:24.40 PS.seek 1.5ml @16:09:30.39 delay 3 seconds @16:09:33.41 PBV.dial between PSV and waste1 @16:09:33.79 PS.seek 0ml @16:09:49.89 delay 10 seconds @16:09:59.92 recoverPSRreagent08 @16:09:59.93 PTV.dial :puck @16:10:00.00 PBV.dial :waste1 @16:10:00.38 delay 30 seconds @16:10:30.41 PBV.dial between PSV and waste1 @16:10:30.78 PSR.select :air @16:10:30.80 PSV.dial PSR @16:10:31.66 PS.reconfigure :fast @16:10:31.71 PS.seek 2.5ml @16:10:55.99 PSV.dial PTV @16:10:56.77 delay 5 seconds @16:11:01.79 PSV.dial PSR @16:11:02.57 delay 5 seconds @16:11:07.59 PSV.dial PTV @16:11:08.35 delay 5 seconds @16:11:13.37 PSV.dial :air @16:11:14.27 PS.seek 0ml @16:11:38.21 Additional air clear @16:11:38.22 PSR.select :air @16:11:38.23 PSV.dial PSR @16:11:39.61 Pulling 2ml of PSR.air @16:11:39.62 PSR.select :air @16:11:39.64 PS.reconfigure :default @16:11:39.69 PS.seek 2ml @16:12:01.17 delay 5 seconds @16:12:06.19 PTV.dial :puck @16:12:06.28 PSV.dial PTV @16:12:07.06 PBV.dial :waste1 @16:12:07.50 PS.seek 1.5ml @16:12:13.48 delay 3 seconds @16:12:16.50 PBV.dial between PSV and waste1 @16:12:16.88 delay 1 second @16:12:17.90 PSV.dial :waste1 @16:12:18.98 PS.seek 0ml @16:12:35.22 Processing Puck Heater at 28C before imaging @16:12:35.24 PH.coast @16:12:35.35 Imaging da puck in Processing Clamp while storing it to tube 3 @16:12:35.57 SC.reconfigure :tight @16:12:35.63 SC.seek 3 @16:12:39.26 SC.configure :limp @16:12:39.49 Elbow.reconfigure :tight @16:12:39.54 Elbow.seek 3 @16:12:41.29 Forearm.reconfigure :tight @16:12:41.34 Forearm.seek 3 + 450 counts @16:12:42.42 SE.reconfigure :pick @16:12:42.52 SE.seek :beamBreak @16:12:44.17 SE.configure :default @16:12:44.33 Elbow.reconfigure :limp @16:12:44.38 Forearm.reconfigure :loose @16:12:44.43 Forearm.seek 3 @16:12:44.70 Elbow.hold @16:12:44.77 Elbow.reconfigure :tight @16:12:44.87 SE.seek 23.12 Pucks below Top Plate @16:12:45.92 Forearm.force -20 @16:12:45.97 Forearm.seek 3 + 305 counts @16:12:46.27 Forearm.reconfigure :tight @16:12:46.32 Forearm.seek 3 + 450 counts @16:12:46.51 SE.seek 24.12 Pucks below Top Plate @16:12:47.13 Forearm.reconfigure :loose @16:12:47.18 Forearm.seek :retract @16:12:48.11 Elbow.seek PC @16:12:50.63 Elbow.reconfigure :loose @16:12:50.74 Forearm.seek PC @16:12:51.72 Hand.close @16:12:52.28 PC.seek :open @16:12:57.09 Forearm.seek :retract @16:12:58.06 Elbow.reconfigure :tight @16:12:58.11 Elbow.seek 3 @16:13:00.75 Forearm.reconfigure :tight @16:13:00.80 Forearm.seek 3 + 450 counts @16:13:01.81 Hand.open @16:13:02.47 SE.seek 23.77 Pucks below Top Plate @16:13:02.75 SE.reconfigure :seal @16:13:02.80 SE.seek 22.8 Pucks below Top Plate @16:13:03.31 Lights, camera, ACTION! @16:13:03.35 PuckCamera: snap -bin=2,2 -auto /var/log/esp/da23nov3016h800ml.tif @16:13:21.11 SX HX9/16 (USB:10): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 14.494 seconds /var/log/esp/da23nov3016h800ml.tif: TIFF Upload Complete @16:13:21.14 PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da23nov3016h800ml15s.tif @16:13:22.85 Waiting >20 seconds for da to relinquish FlushPuck @16:13:36.75 SX HX9/16 (USB:10): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15 seconds /var/log/esp/midres/da23nov3016h800ml15s.tif: TIFF Upload Complete @16:13:36.78 PuckCamera: snap -bin=2,2 20.0 /var/log/esp/midres/da23nov3016h800ml20s.tif @16:13:57.49 SX HX9/16 (USB:10): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 20 seconds /var/log/esp/midres/da23nov3016h800ml20s.tif: TIFF Upload Complete @16:13:57.51 Gate.power :camera,:OFF @16:13:57.53 DAfans.email "14.494 sec auto-exposure",Subject: "da23nov3016h800ml images!" @16:13:57.98 Elbow.seek 3 @16:13:58.20 Forearm.seek 3 + 450 counts @16:13:58.44 SE.reconfigure :gently @16:13:58.50 SE.seek 23.52 Pucks below Top Plate @16:13:59.39 Elbow.reconfigure :limp @16:13:59.47 Forearm.reconfigure :loose @16:13:59.52 Forearm.seek 3 @16:13:59.83 Elbow.hold @16:13:59.91 Elbow.reconfigure :tight @16:13:59.97 SE.reconfigure :default @16:14:00.02 SE.seek 23.7 Pucks below Top Plate @16:14:00.59 Forearm.force -20 @16:14:00.63 Forearm.seek 3 + 296 counts @16:14:01.01 Forearm.reconfigure :tight @16:14:01.06 Forearm.seek 3 + 450 counts @16:14:01.52 SE.seek :down @16:14:02.81 SE.stop @16:14:02.95 Forearm.reconfigure :loose @16:14:03.07 Forearm.seek :retract @16:14:04.30 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @16:14:04.88 Elbow.seek FlushPuck::Garage @16:14:06.80 Elbow.reconfigure :loose @16:14:07.10 Forearm.seek :garage @16:14:08.09 Hand.close @16:14:09.05 Forearm.seek :retract @16:14:10.07 Elbow.reconfigure :tight @16:14:10.18 Elbow.seek PC @16:14:11.56 Elbow.reconfigure :loose @16:14:11.79 Forearm.reconfigure :tight @16:14:11.92 Forearm.seek PC @16:14:14.69 PC.seek :closed @16:14:19.65 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.63 @16:14:19.79 Hand.open @16:14:20.54 Forearm.reconfigure :loose @16:14:20.64 Forearm.seek :retract @16:14:21.70 PSR.select :air @16:14:21.77 Storing wcr puck in Collection Clamp to tube 3 @16:14:21.82 PSV.dial PSR @16:14:22.44 Pulling 0.5ml of PSR.flush @16:14:22.51 PSR.select :flush @16:14:23.01 PS.seek 0.5ml @16:14:23.20 Elbow.reconfigure :tight @16:14:23.31 Elbow.seek 3 @16:14:26.11 Forearm.reconfigure :tight @16:14:26.23 Forearm.seek 3 + 450 counts @16:14:27.38 SE.reconfigure :pick @16:14:27.50 SE.seek :beamBreak @16:14:29.16 delay 5 seconds @16:14:29.20 SE.configure :default @16:14:29.54 Elbow.reconfigure :limp @16:14:29.65 Forearm.reconfigure :loose @16:14:29.75 Forearm.seek 3 @16:14:30.10 Elbow.hold @16:14:30.26 Elbow.reconfigure :tight @16:14:30.47 SE.seek 24.11 Pucks below Top Plate @16:14:32.06 Forearm.force -20 @16:14:32.18 Forearm.seek 3 + 293 counts @16:14:32.67 Forearm.reconfigure :tight @16:14:32.81 Forearm.seek 3 + 450 counts @16:14:33.10 SE.seek 25.11 Pucks below Top Plate @16:14:33.88 Forearm.reconfigure :loose @16:14:33.99 Forearm.seek :retract @16:14:34.22 PSR.select :air @16:14:34.42 PS.seek 2.5ml @16:14:34.95 Elbow.seek CC @16:14:35.42 upsync @16:14:36.59 Elbow.reconfigure :loose @16:14:36.74 Forearm.seek CC @16:14:37.73 Hand.close @16:14:38.41 CC.seek :open @16:14:43.17 Forearm.seek :retract @16:14:44.19 Elbow.reconfigure :tight @16:14:44.24 Elbow.seek 3 @16:14:46.02 Elbow.reconfigure :limp @16:14:46.08 Forearm.seek 3 @16:14:47.12 Elbow.hold @16:14:47.19 Elbow.reconfigure :tight @16:14:47.24 Hand.open @16:14:48.04 Forearm.force -20 @16:14:48.08 Forearm.seek 3 + 317 counts @16:14:48.39 Forearm.reconfigure :tight @16:14:48.82 Forearm.seek 3 + 450 counts @16:14:49.16 SE.seek :down @16:14:50.15 SE.stop @16:14:50.32 Forearm.reconfigure :loose @16:14:50.37 Forearm.seek :retract @16:14:51.35 Starting finalPurge @16:14:55.76 delay 1 second @16:14:56.78 PRV.dial :waste2,avoiding: :ab2 @16:14:58.67 PTV.dial :PRVmixing @16:15:00.00 PSV.dial :mixloop @16:15:01.16 PS.seek :empty @16:15:11.08
Waiting >20 seconds for psFlush to relinquish FlushPuck @16:15:27.51 PSR.select :air @16:15:27.53 PSV.dial PSR @16:15:28.58 Pulling 0.5ml of PSR.flush @16:15:28.59 PSR.select :flush @16:15:28.71 PS.seek 0.5ml @16:15:34.67 delay 5 seconds @16:15:39.69 PSR.select :air @16:15:39.81 PS.seek 2.5ml @16:16:01.14 delay 1 second @16:16:02.16 PBV.dial :waste1 @16:16:02.53 PTV.dial :mixing @16:16:03.28 PSV.dial :mixloop @16:16:04.34 PS.seek :empty @16:16:30.70 PSV.dial PSR @16:16:31.74 PSR.select :flush @16:16:31.87 PS.seek 1ml @16:16:43.10 PSR.select :air @16:16:43.22 PS.seek 2.5ml @16:16:59.39 PSV.dial PTV @16:17:00.18 PTV.dial :puck @16:17:00.96 PBV.dial :waste1 @16:17:01.15 PS.seek 0ml @16:17:27.51 PSV.dial PSR @16:17:28.27 PSR.select :flush @16:17:28.39 PS.seek 0.5ml @16:17:34.32 PSR.select :air @16:17:34.44 PS.seek 2.5ml @16:17:55.73 PSV.dial PTV @16:17:56.49 PBV.dial PSV @16:17:57.06 PS.seek 0ml @16:18:23.44 PSV.dial PBV @16:18:23.92 PS.seek 2.5ml @16:18:50.26 PSV.dial :waste1 @16:18:51.56 PS.seek 0ml @16:19:17.91 PSV.dial PSR @16:19:18.38 PSR.select :air @16:19:18.41 PS.seek 2.5ml @16:19:44.73 PSV.dial PTV @16:19:45.59 PBV.dial PSV @16:19:45.67 PS.seek 0ml @16:20:12.10 PSV.dial PBV @16:20:12.56 PS.seek 2.5ml @16:20:38.94 PSV.dial :waste1 @16:20:39.70 PS.seek 0ml @16:21:06.04 PSV.dial PSR @16:21:06.51 PSR.select :flush @16:21:06.62 PS.seek 1ml @16:21:17.69 PSR.select :air @16:21:17.81 PS.seek 2.5ml @16:21:34.00 PSV.dial PTV @16:21:34.74 PTV.dial :puck @16:21:34.93 PBV.dial :waste1 @16:21:35.50 PS.seek 0ml @16:22:01.88 PSV.dial PSR @16:22:02.63 PSR.select :flush @16:22:02.75 PS.seek 0.5ml @16:22:08.66 PSR.select :air @16:22:08.78 PS.seek 2.5ml @16:22:30.00 PSV.dial PTV @16:22:30.77 PBV.dial PSV @16:22:31.34 PS.seek 0ml @16:22:57.72 PSV.dial PBV @16:22:58.20 PS.seek 2.5ml @16:23:24.60 PSV.dial :waste1 @16:23:25.35 PS.seek 0ml @16:23:51.88 PSV.dial PSR @16:23:52.35 PSR.select :air @16:23:52.37 PS.seek 2.5ml @16:24:18.72 PSV.dial PTV @16:24:19.45 PBV.dial PSV @16:24:19.65 PS.seek 0ml @16:24:46.19 PSV.dial PBV @16:24:46.66 PS.seek 2.5ml @16:25:12.97 PSV.dial :waste1 @16:25:13.73 PS.seek 0ml @16:25:40.29
Loading FlushPuck into Collection Clamp from Processing Clamp @16:25:40.91 Elbow.seek PC @16:25:43.41 Elbow.reconfigure :loose @16:25:43.51 Forearm.seek PC @16:25:44.44 Hand.close @16:25:45.02 PC.seek :open @16:25:49.74 Forearm.seek :retract @16:25:50.76 Elbow.reconfigure :tight @16:25:50.81 Elbow.seek CC @16:25:54.37 Elbow.reconfigure :loose @16:25:54.47 Forearm.reconfigure :tight @16:25:54.52 Forearm.seek CC @16:25:55.82 CC.seek :closed @16:26:00.35 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.49 @16:26:00.42 Hand.open @16:26:01.08 Forearm.reconfigure :loose @16:26:01.13 Forearm.seek :retract @16:26:02.11 Flushing the Collection Valve::Manifold @16:26:02.24 CSV.dial CSR @16:26:02.68 Pulling 4.999ml of CSR.flush @16:26:02.69 CSR.select :flush @16:26:02.81 CS.seek 5ml @16:26:16.41 delay 5 seconds @16:26:21.43 CSR.select :air @16:26:21.60 CS.reconfigure :fast @16:26:21.65 CS.seek 7.5ml @16:26:28.57 CSV.dial :waste @16:26:29.87 CS.reconfigure :default @16:26:29.92 CS.seek 0ml @16:26:50.01 Flushing lower manifold's waste1 port @16:26:50.59 CSV.dial :flush @16:26:51.03 CS.reconfigure :fast @16:26:51.08 CS.seek 5ml @16:27:03.98 delay 5 seconds @16:27:09.00 CSV.dial :air @16:27:09.52 CS.seek 7ml @16:27:15.10 CSV.dial CTV @16:27:15.12
CTV.dial CSV @16:27:16.47 CBV.dial :waste1 @16:27:17.25 CS.seek 0ml @16:27:33.91 Flushing lower manifold's waste2 port @16:27:34.04 CSV.dial :flush @16:27:34.78 CS.seek 5ml @16:27:47.60 delay 5 seconds @16:27:52.62 CSV.dial :air @16:27:53.15 CS.seek 7ml @16:27:58.66 CSV.dial CTV @16:27:58.69
CTV.dial CSV @16:27:59.15 CBV.dial :waste2 @16:28:00.20 CS.seek 0ml @16:28:16.79 Flushing lower manifold's waste2b port @16:28:16.91 CSV.dial :flush @16:28:17.78 CS.seek 5ml @16:28:30.63 delay 5 seconds @16:28:35.65 CSV.dial :air @16:28:36.15 CS.seek 7ml @16:28:41.76 CSV.dial CTV @16:28:41.78
CTV.dial CSV @16:28:42.24 CBV.dial :waste2b @16:28:43.29 CS.seek 0ml @16:28:59.82 Disinfecting external Intake with kill @16:28:59.94 CSV.dial CSR @16:29:00.51 Pulling 8ml of CSR.kill CSR.select :kill @16:29:00.63 CS.seek 8ml @16:29:20.47 delay 5 seconds @16:29:25.49 CSR.select :air @16:29:25.61 CS.seek 10ml @16:29:31.13 CSV.dial CBV @16:29:31.15
CBV.dial CSV @16:29:32.25 CTV.dial :intake @16:29:33.03 Intake.select :open @16:29:33.15 CS.seek 2ml @16:29:52.06 Intake.select :close @16:29:52.17 CTV.dial :air @16:29:53.37 CS.reconfigure :default @16:29:53.42 CS.seek 0ml @16:29:59.28 CSV.dial CSR @16:30:00.43 Pulling 5ml of CSR.flush CSR.select :flush @16:30:00.55 CS.seek 5ml @16:30:14.16 delay 5 seconds @16:30:19.18 CSR.select :air @16:30:19.30 CS.seek 10ml @16:30:32.97 CSV.dial CBV @16:30:33.98 CS.seek 0ml @16:31:00.44 Garaging FlushPuck in Collection Clamp @16:31:00.51 Elbow.reconfigure :tight @16:31:00.56 Elbow.seek CC @16:31:00.89 Elbow.reconfigure :loose @16:31:00.99 Forearm.seek CC @16:31:02.05 Hand.close @16:31:02.65 CC.seek :open @16:31:07.28 Forearm.seek :retract @16:31:08.25 Elbow.reconfigure :tight @16:31:08.30 Elbow.seek :garage @16:31:11.18 Elbow.reconfigure :loose @16:31:11.28 Forearm.reconfigure :tight @16:31:11.33 Forearm.seek :garage @16:31:12.38 Hand.open @16:31:13.08 Forearm.reconfigure :loose @16:31:13.12 Forearm.seek :retract @16:31:14.13 Completed [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] begun 13:59:25 @16:31:14.18 Asleep after completing DA_WCR_113023 assays @16:31:14.20 GoodNews.email "{1 => 33,\n 2 => 33,\n 3 => 26,\n 4 => 7,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@16:28:48, 30% flow, 16.8C, 29% humidity, 15.2psia, 13.691V, 0.441A, 116.209Ah, 6.04W\nSampled 425.0ml",Subject: "Asleep after completing DA_WCR_113023 assays" @16:31:18.26 upsync + ESPwhich DA_WCR_113023b.rb + fn=DA_WCR_113023b.rb + IFS=: + set /home/esp/esp2/mission . + fullPath=/home/esp/esp2/mission/DA_WCR_113023b.rb + [ -f /home/esp/esp2/mission/DA_WCR_113023b.rb ] + realpath /home/esp/esp2/mission/DA_WCR_113023b.rb + return 0 + scriptPath=/home/esp/esp2local/mission/DA_WCR_113023b.rb + exec ruby /home/esp/esp2/lib/esp.rb /home/esp/esp2local/mission/DA_WCR_113023b.rb @20:55:28.46UTC30-Nov-23
ESP2 v3.42 I2C Gateway v3.20 for core bus @20:55:31.15 Configuration for short puck jake 1/11/21 by brent@mbari.org /home/esp/esp2/type/shallow/jake/configure.rb @20:55:34.80 Polling Can every 10:00 @20:55:34.93 Cleanly exited at 19:33:46UTC30-Nov-23 @20:55:34.98 Loading /home/esp/esp2local/mission/DA_WCR_113023b.rb mission @20:55:35.04 GoodNews.email "Starting DA_WCR_113023b w/pucks in tube 4\n{1 => 33,\n 2 => 33,\n 3 => 26,\n 4 => 7,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@20:55:34, 31% flow, 16.9C, 30% humidity, 15.2psia, 13.734V, 0.302A, 117.436Ah, 4.15W",Subject: "DA_WCR_113023b begun" @20:55:35.07
Preparing to sleep until + 00:00:05 @20:55:35.12 Asleep til + 00:00:05 @20:55:35.13 Continuing at 20:55 @20:55:35.42 Gate.power :core,:OFF @20:55:35.43 Gate.power :raw,:OFF @20:55:35.44 Shutdown @20:55:37.92 GoodNews.email "Then, in tube 4: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_113023b.rb:14\n{1 => 33,\n 2 => 33,\n 3 => 26,\n 4 => 7,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@20:55:35, 31% flow, 16.9C, 30% humidity, 15.2psia, 13.734V, 0.309A, 117.436Ah, 4.24W",Subject: "Asleep until 20:55" @20:55:39.25 upsync @20:55:40.01
Powering core bus @20:55:41.78 upsync @20:55:45.49
Gate.power :raw,:ON @20:55:46.26 CS.configure :default @20:55:46.30 PS.configure :default @20:55:46.35 SS.configure :default @20:55:46.40 CC.configure :default @20:55:46.45 PC.configure :default @20:55:46.52 Forearm.configure :loose @20:55:46.57 Elbow.configure :loose @20:55:46.64 SC.configure :loose @20:55:46.69 SE.configure :default @20:55:46.73 CH.configure :default @20:55:46.77 PH.configure :default @20:55:46.81 All dwarf controllers running firmware version 3.85 @20:55:46.92 Valve.reset @20:55:47.18 Intake.select :close @20:55:47.24 Exhaust.select :close @20:55:47.25 CTV.dial :air @20:55:47.34 PSV.dial :air @20:55:47.37 CSV.dial :waste1 @20:55:47.52
Forearm.home.seek :retract @20:55:47.88 SSV.dial :spit,avoiding: :exhaust @20:55:48.29 Hand.close @20:55:48.32
Elbow.reconfigure :loose @20:55:48.41 Elbow.jog 1500 @20:55:48.48 PS.home.seek 0ml @20:55:48.78 CS.reconfigure :fast @20:55:48.82 SS.home.seek 0ml @20:55:48.92 CS.home.seek 0ml @20:55:49.01
Elbow.reconfigure :loose @20:55:49.05 Elbow.home.seek :clear @20:55:52.36 SE.home.seek :down @20:55:52.99 SC.reconfigure :tight @20:55:53.04 SC.home.seek :initial @20:55:56.97 PS.stop @20:56:08.80
SC.configure :limp @20:56:08.85 Forearm.seek :retract @20:56:08.92 Elbow.seek :hammer @20:56:09.96 Forearm.reconfigure :tight @20:56:10.02 Forearm.seek retracted @20:56:10.71 PC.home.seek :open @20:56:10.78
CC.home.seek :open @20:56:14.20 Phase [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] @20:56:14.22 Notice.email "At 20:56:14 began in tube 4: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_113023b.rb:14\n{1 => 33,\n 2 => 33,\n 3 => 26,\n 4 => 7,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@20:56:14, 31% flow, 16.8C, 30% humidity, 15.2psia, 13.705V, 0.278A, 117.439Ah, 3.81W",Subject: "Started DA_WCR_113023b phase initialPurge; da [1000, 1000] #da and wsr are run simultanelously" @20:56:14.23
Starting initialPurge @20:56:14.26 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @20:56:14.33 Hand.open @20:56:15.08 Elbow.reconfigure :tight @20:56:15.13 Elbow.seek FlushPuck::Garage @20:56:17.18 Elbow.reconfigure :loose @20:56:17.31 Forearm.reconfigure :loose @20:56:17.35 Forearm.seek :garage @20:56:18.23 upsync @20:56:18.32
Hand.close @20:56:19.08 Forearm.seek :retract @20:56:20.06 Elbow.reconfigure :tight @20:56:20.11 Elbow.seek CC @20:56:23.07 Elbow.reconfigure :loose @20:56:23.17 Forearm.reconfigure :tight @20:56:23.27 Forearm.seek CC @20:56:24.55 CC.seek :closed @20:56:29.09 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.49 @20:56:29.16 Hand.open @20:56:29.90 Forearm.reconfigure :loose @20:56:29.95 Forearm.seek :retract @20:56:30.93 Flushing the Collection Valve::Manifold @20:56:31.06 CSV.dial CSR @20:56:32.35 Pulling 4.999ml of CSR.flush @20:56:32.36 CSR.select :flush @20:56:32.48 CS.reconfigure :default @20:56:32.53 CS.seek 5ml @20:56:46.74 delay 5 seconds @20:56:51.76 CSR.select :air @20:56:51.92 CS.reconfigure :fast @20:56:51.97 CS.seek 7.5ml @20:56:58.89 CSV.dial :waste @20:57:00.18 CS.reconfigure :default @20:57:00.23 CS.seek 0ml @20:57:20.30 Purging external Intake @20:57:20.43 CSV.dial :flush @20:57:20.88 CS.reconfigure :fast @20:57:20.93 CS.seek 10ml @20:57:45.33 delay 5 seconds @20:57:50.35 CSV.dial CBV @20:57:50.38
CBV.dial CSV @20:57:51.25 CTV.dial :intake @20:57:52.05 Intake.select :open @20:57:52.16 CS.seek 0ml @20:58:15.61 Intake.select :close @20:58:15.72 CTV.dial :air @20:58:16.51 Flushing upper manifold @20:58:16.62 CSV.dial :flush @20:58:17.35 CS.seek 5ml @20:58:30.24 delay 5 seconds @20:58:35.26 CSV.dial :air @20:58:35.76 CS.seek 7ml @20:58:41.30 CSV.dial CBV @20:58:41.32
CBV.dial CSV @20:58:42.38 CTV.dial :air @20:58:42.46 CS.seek 0ml @20:58:59.02 delay 7 seconds @20:59:06.05 CS.seek 3ml @20:59:14.11 CSV.dial :waste @20:59:14.57 CS.seek 0ml @20:59:22.35 CSV.dial :flush @20:59:22.91 CS.seek 3ml @20:59:30.99 delay 5 seconds @20:59:36.00 CSV.dial :air @20:59:36.53 CS.seek 5ml @20:59:42.17 CSV.dial :air @20:59:42.35 CS.seek 0ml @20:59:54.45 delay 7 seconds @21:00:01.48 CS.seek 3ml @21:00:09.55 CSV.dial :waste @21:00:10.29 CS.seek 0ml @21:00:17.89 Garaging FlushPuck in Collection Clamp @21:00:17.96 Elbow.reconfigure :tight @21:00:18.01 Elbow.seek CC @21:00:18.33 Elbow.reconfigure :loose @21:00:18.43 Forearm.seek CC @21:00:19.38 Hand.close @21:00:19.95 CC.seek :open @21:00:25.01 Forearm.seek :retract @21:00:25.98 Elbow.reconfigure :tight @21:00:26.02 Elbow.seek :garage @21:00:28.94 Elbow.reconfigure :loose @21:00:29.04 Forearm.reconfigure :tight @21:00:29.09 Forearm.seek :garage @21:00:30.04 Hand.open @21:00:30.75 Forearm.reconfigure :loose @21:00:30.80 Forearm.seek :retract @21:00:31.79 da sampling at most 1000ml, wcr at most 1000ml @21:00:31.83 Loading sh1 puck from tube 4 into Collection Clamp @21:00:31.85 DAfans.email "Started da in tube 4 at 21:00:31UTC30-Nov-23\n{1 => 33,\n 2 => 33,\n 3 => 26,\n 4 => 7,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@20:56:14, 31% flow, 16.8C, 30% humidity, 15.2psia, 13.705V, 0.278A, 117.439Ah, 3.81W",Subject: "da sampling at most 1000ml, wcr at most 1000ml" @21:00:32.11
SC.reconfigure :tight @21:00:32.20 SC.seek 4 @21:00:32.33 SC.configure :limp @21:00:32.58 Elbow.reconfigure :tight @21:00:32.63 Elbow.seek 4 @21:00:34.59 Forearm.reconfigure :tight @21:00:34.64 Forearm.seek 4 + 450 counts @21:00:35.77 SE.reconfigure :pick @21:00:35.84 SE.seek :beamBreak @21:00:36.28 upsync @21:00:38.55
SE.configure :default @21:00:38.72 Elbow.seek 4 @21:00:38.92 Forearm.seek 4 + 300 counts @21:00:39.11 Forearm.reconfigure :loose @21:00:39.16 Hand.close @21:00:39.83 SE.seek :down @21:00:40.43
Forearm.seek :retract @21:00:41.45 Elbow.seek CC @21:00:42.25 SE.stop @21:00:43.08
Elbow.reconfigure :loose @21:00:43.18 Forearm.reconfigure :tight @21:00:43.23 Forearm.seek CC @21:00:44.48 CC.seek :closed @21:00:49.00 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.36 @21:00:49.01 Hand.open @21:00:49.63 Forearm.reconfigure :loose @21:00:49.68 Forearm.seek :retract @21:00:50.63 collectSample 1000ml w/bubble point of 24psi @21:00:50.64 Priming 7ml @21:00:50.65 Intake.select :close @21:00:50.66 Exhaust.select :close @21:00:50.68 SS.hold @21:00:50.88 Calibrating pressure and temperature sensors @21:00:50.91 CTV.dial :air @21:00:50.99 SSV.dial :spit,avoiding: :exhaust @21:00:51.07 SS.reconfigure :fast @21:00:51.12 SS.seek 5ml @21:00:53.97 SSV.dial CBV @21:00:54.00
CBV.dial SSV @21:00:55.34 delay 3.8 seconds @21:00:59.27 Sensor limits: Intake max 103.4psia; Outlet max 96.1psia @21:00:59.28 SSV.dial :spit @21:01:00.60 SS.seek 0ml @21:01:03.46 SSV.dial CBV @21:01:04.78 CTV.dial :intake @21:01:05.56 SSV.dial CBV @21:01:05.61
CBV.dial SSV @21:01:05.78 Allowing 40s for 7.0ml of remaining 7.0ml @21:01:05.79 Intake.select :open @21:01:07.96 Intake @14.6psia, vacuum from 9.1 to 12.1psi @21:01:07.97 SS.reconfigure :inhaling 2.20ml/s @21:01:08.02 SS.seek 7ml @21:01:15.02 Stroke completed -- waiting for pressure to equalize ... @21:01:15.03 SS.reconfigure :exhale @21:01:15.08 SS.hold @21:01:22.78 Intake.select :close @21:01:22.93 SSV.dial :exhaust @21:01:23.68 Exhaust.select :open @21:01:23.80 SS.seek 0ml @21:01:27.95 Exhaust.select :close @21:01:28.06 Sampling 1000.0ml @21:01:28.07 Intake.select :close @21:01:28.08 Exhaust.select :close @21:01:28.10 SS.reconfigure :default @21:01:28.14 SS.hold @21:01:28.35 CTV.dial :intake @21:01:28.52 SSV.dial CBV @21:01:28.54
CBV.dial SSV @21:01:29.31 Allowing 130s for 25.0ml of remaining 1000.0ml @21:01:29.32 Intake.select :open @21:01:31.49 Intake @15.5psia, vacuum from 10.0 to 13.0psi @21:01:31.51 SS.reconfigure :inhaling 2.20ml/s @21:01:31.56 SS.seek 25ml @21:01:39.80 Vacuum too high -- waiting up to 40 seconds @21:01:39.81 SS.reconfigure :exhale @21:01:39.87 SS.hold @21:01:45.07 Intake @17.1psia, vacuum from 10.0 to 13.0psi @21:01:45.09 SS.reconfigure :inhaling 1.76ml/s @21:01:45.14 SS.seek 25ml @21:01:48.22 Vacuum too high -- waiting up to 40 seconds @21:01:48.23 SS.reconfigure :exhale @21:01:48.28 SS.hold @21:01:50.49 Intake @17.4psia, vacuum from 10.0 to 13.0psi @21:01:50.50 SS.reconfigure :inhaling 1.41ml/s @21:01:50.55 SS.seek 25ml @21:01:54.14 Vacuum too high -- waiting up to 40 seconds @21:01:54.16 SS.reconfigure :exhale @21:01:54.21 SS.hold @21:01:56.37 Intake @17.9psia, vacuum from 10.0 to 13.0psi @21:01:56.39 SS.reconfigure :inhaling 1.13ml/s @21:01:56.44 SS.seek 25ml @21:02:00.02 Vacuum too high -- waiting up to 40 seconds @21:02:00.03 SS.reconfigure :exhale @21:02:00.08 SS.hold @21:02:01.42 Intake @17.5psia, vacuum from 10.0 to 13.0psi @21:02:01.44 SS.reconfigure :inhaling 0.91ml/s @21:02:01.50 SS.seek 25ml @21:02:03.59 Stroke completed -- waiting for pressure to equalize ... @21:02:03.60 SS.reconfigure :exhale @21:02:03.64 SS.hold @21:02:34.19 Intake.select :close @21:02:34.34 SSV.dial :exhaust @21:02:35.10 Exhaust.select :open @21:02:35.22 SS.seek 0ml @21:02:44.07 Exhaust.select :close @21:02:44.18 SSV.dial CBV @21:02:44.20
CBV.dial SSV @21:02:44.98 Allowing 130s for 25.0ml of remaining 975.0ml @21:02:44.99 Intake.select :open @21:02:47.16 Intake @15.8psia, vacuum from 10.0 to 13.0psi @21:02:47.17 SS.reconfigure :inhaling 0.91ml/s @21:02:47.22 SS.seek 25ml @21:03:17.69 Stroke completed -- waiting for pressure to equalize ... @21:03:17.70 SS.reconfigure :exhale @21:03:17.75 SS.hold @21:03:50.48 Intake.select :close @21:03:50.64 SSV.dial :exhaust @21:03:51.41 Exhaust.select :open @21:03:51.52 SS.seek 0ml @21:04:00.24 Exhaust.select :close @21:04:00.35 SSV.dial CBV @21:04:00.37
CBV.dial SSV @21:04:01.13 Allowing 130s for 25.0ml of remaining 950.0ml @21:04:01.15 Intake.select :open @21:04:03.31 Intake @15.9psia, vacuum from 10.0 to 13.0psi @21:04:03.32 SS.reconfigure :inhaling 0.91ml/s @21:04:03.37 SS.seek 25ml @21:04:33.24 Stroke completed -- waiting for pressure to equalize ... @21:04:33.25 SS.reconfigure :exhale @21:04:33.30 SS.hold @21:05:04.09 Intake.select :close @21:05:04.25 SSV.dial :exhaust @21:05:05.01 Exhaust.select :open @21:05:05.12 SS.seek 0ml @21:05:14.10 Exhaust.select :close @21:05:14.21 SSV.dial CBV @21:05:14.23
CBV.dial SSV @21:05:15.01 Allowing 130s for 25.0ml of remaining 925.0ml @21:05:15.02 Intake.select :open @21:05:17.19 Intake @15.9psia, vacuum from 10.0 to 13.0psi @21:05:17.20 SS.reconfigure :inhaling 0.91ml/s @21:05:17.25 SS.seek 25ml @21:05:46.64 Stroke completed -- waiting for pressure to equalize ... @21:05:46.66 SS.reconfigure :exhale @21:05:46.70 SS.hold @21:06:15.43 Intake.select :close @21:06:15.59 SSV.dial :exhaust @21:06:16.35 Exhaust.select :open @21:06:16.46 SS.seek 0ml @21:06:25.41 Exhaust.select :close @21:06:25.52 SSV.dial CBV @21:06:25.54
CBV.dial SSV @21:06:26.30 Allowing 130s for 25.0ml of remaining 900.0ml @21:06:26.31 Intake.select :open @21:06:28.48 Intake @15.9psia, vacuum from 10.0 to 13.0psi @21:06:28.50 SS.reconfigure :inhaling 0.91ml/s @21:06:28.55 SS.seek 25ml @21:06:58.03 Stroke completed -- waiting for pressure to equalize ... @21:06:58.04 SS.reconfigure :exhale @21:06:58.09 SS.hold @21:07:22.82 Intake.select :close @21:07:22.98 SSV.dial :exhaust @21:07:23.74 Exhaust.select :open @21:07:23.85 SS.seek 0ml @21:07:32.53 Exhaust.select :close @21:07:32.65 SSV.dial CBV @21:07:32.67
CBV.dial SSV @21:07:33.44 Allowing 130s for 25.0ml of remaining 875.0ml @21:07:33.45 Intake.select :open @21:07:35.62 Intake @16.0psia, vacuum from 10.0 to 13.0psi @21:07:35.64 SS.reconfigure :inhaling 0.91ml/s @21:07:35.69 SS.seek 25ml @21:08:05.55 Stroke completed -- waiting for pressure to equalize ... @21:08:05.56 SS.reconfigure :exhale @21:08:05.61 SS.hold @21:08:26.09 Intake.select :close @21:08:26.25 SSV.dial :exhaust @21:08:27.01 Exhaust.select :open @21:08:27.13 SS.seek 0ml @21:08:35.92 Exhaust.select :close @21:08:36.03 SSV.dial CBV @21:08:36.07
CBV.dial SSV @21:08:36.80 Allowing 130s for 25.0ml of remaining 850.0ml @21:08:36.81 Intake.select :open @21:08:38.98 Intake @16.1psia, vacuum from 10.0 to 13.0psi @21:08:39.00 SS.reconfigure :inhaling 0.91ml/s @21:08:39.05 SS.seek 25ml @21:09:09.09 Stroke completed -- waiting for pressure to equalize ... @21:09:09.10 SS.reconfigure :exhale @21:09:09.15 SS.hold @21:09:27.92 Intake.select :close @21:09:28.08 SSV.dial :exhaust @21:09:28.85 Exhaust.select :open @21:09:28.96 SS.seek 0ml @21:09:37.72 Exhaust.select :close @21:09:37.83 SSV.dial CBV @21:09:37.85
CBV.dial SSV @21:09:38.63 Allowing 130s for 25.0ml of remaining 825.0ml @21:09:38.64 Intake.select :open @21:09:40.81 Intake @16.2psia, vacuum from 10.0 to 13.0psi @21:09:40.82 SS.reconfigure :inhaling 0.91ml/s @21:09:40.87 SS.seek 25ml @21:10:10.42 Stroke completed -- waiting for pressure to equalize ... @21:10:10.43 SS.reconfigure :exhale @21:10:10.48 SS.hold @21:10:31.31 Intake.select :close @21:10:31.46 SSV.dial :exhaust @21:10:32.23 Exhaust.select :open @21:10:32.34 SS.seek 0ml @21:10:41.03 Exhaust.select :close @21:10:41.14 SSV.dial CBV @21:10:41.16
CBV.dial SSV @21:10:41.93 Allowing 130s for 25.0ml of remaining 800.0ml @21:10:41.94 Intake.select :open @21:10:44.10 Intake @16.0psia, vacuum from 10.0 to 13.0psi @21:10:44.11 SS.reconfigure :inhaling 0.91ml/s @21:10:44.16 SS.seek 25ml @21:11:14.11 Stroke completed -- waiting for pressure to equalize ... @21:11:14.12 SS.reconfigure :exhale @21:11:14.16 SS.hold @21:11:32.93 Intake.select :close @21:11:33.09 SSV.dial :exhaust @21:11:33.84 Exhaust.select :open @21:11:33.96 SS.seek 0ml @21:11:42.69 Exhaust.select :close @21:11:42.80 SSV.dial CBV @21:11:42.82
CBV.dial SSV @21:11:43.59 Allowing 130s for 25.0ml of remaining 775.0ml @21:11:43.61 Intake.select :open @21:11:45.77 Intake @16.0psia, vacuum from 10.0 to 13.0psi @21:11:45.79 SS.reconfigure :inhaling 0.91ml/s @21:11:45.84 SS.seek 25ml @21:12:15.81 Stroke completed -- waiting for pressure to equalize ... @21:12:15.82 SS.reconfigure :exhale @21:12:15.87 SS.hold @21:12:36.30 Intake.select :close @21:12:36.46 SSV.dial :exhaust @21:12:37.22 Exhaust.select :open @21:12:37.34 SS.seek 0ml @21:12:46.53 Exhaust.select :close @21:12:46.64 SSV.dial CBV @21:12:46.66
CBV.dial SSV @21:12:47.44 Allowing 130s for 25.0ml of remaining 750.0ml @21:12:47.45 Intake.select :open @21:12:49.62 Intake @16.0psia, vacuum from 10.0 to 13.0psi @21:12:49.64 SS.reconfigure :inhaling 0.91ml/s @21:12:49.69 SS.seek 25ml @21:13:19.70 Stroke completed -- waiting for pressure to equalize ... @21:13:19.71 SS.reconfigure :exhale @21:13:19.76 SS.hold @21:13:38.13 Intake.select :close @21:13:38.29 SSV.dial :exhaust @21:13:39.06 Exhaust.select :open @21:13:39.18 SS.seek 0ml @21:13:47.97 Exhaust.select :close @21:13:48.08 SSV.dial CBV @21:13:48.10
CBV.dial SSV @21:13:48.88 Allowing 130s for 25.0ml of remaining 725.0ml @21:13:48.89 Intake.select :open @21:13:51.06 Intake @16.1psia, vacuum from 10.0 to 13.0psi @21:13:51.08 SS.reconfigure :inhaling 0.91ml/s @21:13:51.12 SS.seek 25ml @21:14:21.26 Stroke completed -- waiting for pressure to equalize ... @21:14:21.27 SS.reconfigure :exhale @21:14:21.32 SS.hold @21:14:39.94 Intake.select :close @21:14:40.09 SSV.dial :exhaust @21:14:40.86 Exhaust.select :open @21:14:40.97 SS.seek 0ml @21:14:49.87 Exhaust.select :close @21:14:49.98 SSV.dial CBV @21:14:50.00
CBV.dial SSV @21:14:50.78 Allowing 130s for 25.0ml of remaining 700.0ml @21:14:50.79 Intake.select :open @21:14:52.96 Intake @16.2psia, vacuum from 10.0 to 13.0psi @21:14:52.97 SS.reconfigure :inhaling 0.91ml/s @21:14:53.02 SS.seek 25ml @21:15:22.63 Stroke completed -- waiting for pressure to equalize ... @21:15:22.64 SS.reconfigure :exhale @21:15:22.69 SS.hold @21:15:43.27 Intake.select :close @21:15:43.42 SSV.dial :exhaust @21:15:44.21 Exhaust.select :open @21:15:44.32 SS.seek 0ml @21:15:53.09 Exhaust.select :close @21:15:53.19 SSV.dial CBV @21:15:53.23
CBV.dial SSV @21:15:53.98 Allowing 130s for 25.0ml of remaining 675.0ml @21:15:53.99 Intake.select :open @21:15:56.16 Intake @16.1psia, vacuum from 10.0 to 13.0psi @21:15:56.17 SS.reconfigure :inhaling 0.91ml/s @21:15:56.22 SS.seek 25ml @21:16:26.16 Stroke completed -- waiting for pressure to equalize ... @21:16:26.17 SS.reconfigure :exhale @21:16:26.22 SS.hold @21:16:48.74 Intake.select :close @21:16:48.90 SSV.dial :exhaust @21:16:49.67 Exhaust.select :open @21:16:49.78 SS.seek 0ml @21:16:58.54 Exhaust.select :close @21:16:58.65 SSV.dial CBV @21:16:58.68
CBV.dial SSV @21:16:59.45 Allowing 130s for 25.0ml of remaining 650.0ml @21:16:59.46 Intake.select :open @21:17:01.63 Intake @15.9psia, vacuum from 10.0 to 13.0psi @21:17:01.64 SS.reconfigure :inhaling 0.91ml/s @21:17:01.69 SS.seek 25ml @21:17:31.52 Stroke completed -- waiting for pressure to equalize ... @21:17:31.53 SS.reconfigure :exhale @21:17:31.58 SS.hold @21:17:52.40 Intake.select :close @21:17:52.55 SSV.dial :exhaust @21:17:53.33 Exhaust.select :open @21:17:53.44 SS.seek 0ml @21:18:02.40 Exhaust.select :close @21:18:02.50 SSV.dial CBV @21:18:02.54
CBV.dial SSV @21:18:03.28 Allowing 130s for 25.0ml of remaining 625.0ml @21:18:03.29 Intake.select :open @21:18:05.45 Intake @16.0psia, vacuum from 10.0 to 13.0psi @21:18:05.47 SS.reconfigure :inhaling 0.91ml/s @21:18:05.51 SS.seek 25ml @21:18:35.00 Stroke completed -- waiting for pressure to equalize ... @21:18:35.01 SS.reconfigure :exhale @21:18:35.07 SS.hold @21:18:57.99 Intake.select :close @21:18:58.14 SSV.dial :exhaust @21:18:58.90 Exhaust.select :open @21:18:59.01 SS.seek 0ml @21:19:08.18 Exhaust.select :close @21:19:08.29 SSV.dial CBV @21:19:08.31
CBV.dial SSV @21:19:09.09 Allowing 130s for 25.0ml of remaining 600.0ml @21:19:09.11 Intake.select :open @21:19:11.27 Intake @16.1psia, vacuum from 10.0 to 13.0psi @21:19:11.28 SS.reconfigure :inhaling 0.91ml/s @21:19:11.33 SS.seek 25ml @21:19:40.33 Stroke completed -- waiting for pressure to equalize ... @21:19:40.34 SS.reconfigure :exhale @21:19:40.39 SS.hold @21:20:05.35 Intake.select :close @21:20:05.51 SSV.dial :exhaust @21:20:06.29 Exhaust.select :open @21:20:06.40 SS.seek 0ml @21:20:15.19 Exhaust.select :close @21:20:15.30 SSV.dial CBV @21:20:15.34
CBV.dial SSV @21:20:16.09 Allowing 130s for 25.0ml of remaining 575.0ml @21:20:16.10 Intake.select :open @21:20:18.27 Intake @16.0psia, vacuum from 10.0 to 13.0psi @21:20:18.29 SS.reconfigure :inhaling 0.91ml/s @21:20:18.34 SS.seek 25ml @21:20:48.14 Stroke completed -- waiting for pressure to equalize ... @21:20:48.15 SS.reconfigure :exhale @21:20:48.20 SS.hold @21:21:14.70 Intake.select :close @21:21:14.86 SSV.dial :exhaust @21:21:15.64 Exhaust.select :open @21:21:15.76 SS.seek 0ml @21:21:24.52 Exhaust.select :close @21:21:24.63 SSV.dial CBV @21:21:24.65
CBV.dial SSV @21:21:25.43 Allowing 130s for 25.0ml of remaining 550.0ml @21:21:25.44 Intake.select :open @21:21:27.61 Intake @16.0psia, vacuum from 10.0 to 13.0psi @21:21:27.62 SS.reconfigure :inhaling 0.91ml/s @21:21:27.67 SS.seek 25ml @21:21:56.37 Vacuum too high -- waiting up to 40 seconds @21:21:56.38 SS.reconfigure :exhale @21:21:56.44 SS.hold @21:22:10.98 Intake @18.1psia, vacuum from 10.0 to 13.0psi @21:22:11.00 SS.reconfigure :inhaling 0.73ml/s @21:22:11.05 SS.seek 25ml @21:22:13.33 Stroke completed -- waiting for pressure to equalize ... @21:22:13.34 SS.reconfigure :exhale @21:22:13.39 SS.hold @21:22:24.54 Intake.select :close @21:22:24.69 SSV.dial :exhaust @21:22:25.47 Exhaust.select :open @21:22:25.59 SS.seek 0ml @21:22:34.33 Exhaust.select :close @21:22:34.44 SSV.dial CBV @21:22:34.46
CBV.dial SSV @21:22:35.24 Allowing 130s for 25.0ml of remaining 525.0ml @21:22:35.25 Intake.select :open @21:22:37.42 Intake @16.1psia, vacuum from 10.0 to 13.0psi @21:22:37.43 SS.reconfigure :inhaling 0.73ml/s @21:22:37.48 SS.seek 25ml @21:23:14.05 Stroke completed -- waiting for pressure to equalize ... @21:23:14.06 SS.reconfigure :exhale @21:23:14.11 SS.hold @21:23:38.55 Intake.select :close @21:23:38.71 SSV.dial :exhaust @21:23:39.47 Exhaust.select :open @21:23:39.59 SS.seek 0ml @21:23:48.53 Exhaust.select :close @21:23:48.64 SSV.dial CBV @21:23:48.67
CBV.dial SSV @21:23:49.44 Allowing 130s for 25.0ml of remaining 500.0ml @21:23:49.45 Intake.select :open @21:23:51.62 Intake @16.0psia, vacuum from 10.0 to 13.0psi @21:23:51.63 SS.reconfigure :inhaling 0.73ml/s @21:23:51.68 SS.seek 25ml @21:24:27.62 Stroke completed -- waiting for pressure to equalize ... @21:24:27.63 SS.reconfigure :exhale @21:24:27.68 SS.hold @21:24:54.64 Intake.select :close @21:24:54.80 SSV.dial :exhaust @21:24:55.57 Exhaust.select :open @21:24:55.69 SS.seek 0ml @21:25:04.42 Exhaust.select :close @21:25:04.53 SSV.dial CBV @21:25:04.55
CBV.dial SSV @21:25:05.32 Allowing 130s for 25.0ml of remaining 475.0ml @21:25:05.34 Intake.select :open @21:25:07.50 Intake @16.2psia, vacuum from 10.0 to 13.0psi @21:25:07.52 SS.reconfigure :inhaling 0.73ml/s @21:25:07.57 SS.seek 25ml @21:25:42.62 Vacuum too high -- waiting up to 40 seconds @21:25:42.63 SS.reconfigure :exhale @21:25:42.69 SS.hold @21:25:55.55 Intake @17.2psia, vacuum from 10.0 to 13.0psi @21:25:55.57 SS.reconfigure :inhaling 0.59ml/s @21:25:55.62 SS.seek 25ml @21:25:56.87 Stroke completed -- waiting for pressure to equalize ... @21:25:56.88 SS.reconfigure :exhale @21:25:56.93 SS.hold @21:26:11.35 Intake.select :close @21:26:11.94 SSV.dial :exhaust @21:26:12.70 Exhaust.select :open @21:26:12.82 SS.seek 0ml @21:26:21.97 Exhaust.select :close @21:26:22.07 SSV.dial CBV @21:26:22.11
CBV.dial SSV @21:26:22.86 Allowing 130s for 25.0ml of remaining 450.0ml @21:26:22.87 Intake.select :open @21:26:25.04 Intake @16.1psia, vacuum from 10.0 to 13.0psi @21:26:25.06 SS.reconfigure :inhaling 0.59ml/s @21:26:25.10 SS.seek 25ml @21:27:08.75 Stroke completed -- waiting for pressure to equalize ... @21:27:08.76 SS.reconfigure :exhale @21:27:08.80 SS.hold @21:27:33.19 Intake.select :close @21:27:33.78 SSV.dial :exhaust @21:27:35.31 Exhaust.select :open @21:27:35.43 SS.seek 0ml @21:27:44.40 Exhaust.select :close @21:27:44.52 SSV.dial CBV @21:27:44.54
CBV.dial SSV @21:27:45.31 Allowing 130s for 25.0ml of remaining 425.0ml @21:27:45.32 Intake.select :open @21:27:47.49 Intake @16.1psia, vacuum from 10.0 to 13.0psi @21:27:47.51 SS.reconfigure :inhaling 0.59ml/s @21:27:47.56 SS.seek 25ml @21:28:31.84 Stroke completed -- waiting for pressure to equalize ... @21:28:31.85 SS.reconfigure :exhale @21:28:31.90 SS.hold @21:28:58.47 Intake.select :close @21:28:59.05 SSV.dial :exhaust @21:28:59.81 Exhaust.select :open @21:28:59.92 SS.seek 0ml @21:29:08.92 Exhaust.select :close @21:29:09.02 SSV.dial CBV @21:29:09.06
CBV.dial SSV @21:29:09.79 Allowing 130s for 25.0ml of remaining 400.0ml @21:29:09.81 Intake.select :open @21:29:11.97 Intake @16.2psia, vacuum from 10.0 to 13.0psi @21:29:11.99 SS.reconfigure :inhaling 0.59ml/s @21:29:12.03 SS.seek 25ml @21:29:56.52 Stroke completed -- waiting for pressure to equalize ... @21:29:56.53 SS.reconfigure :exhale @21:29:56.58 SS.hold @21:30:27.22 Intake.select :close @21:30:27.37 SSV.dial :exhaust @21:30:28.14 Exhaust.select :open @21:30:28.25 SS.seek 0ml @21:30:37.07 Exhaust.select :close @21:30:37.18 SSV.dial CBV @21:30:37.21
CBV.dial SSV @21:30:38.54 Allowing 130s for 25.0ml of remaining 375.0ml @21:30:38.55 Intake.select :open @21:30:40.72 Intake @16.2psia, vacuum from 10.0 to 13.0psi @21:30:40.74 SS.reconfigure :inhaling 0.59ml/s @21:30:40.79 SS.seek 25ml @21:31:19.70 Vacuum too high -- waiting up to 40 seconds @21:31:19.72 SS.reconfigure :exhale @21:31:19.77 SS.hold @21:31:34.29 Intake @18.2psia, vacuum from 10.0 to 13.0psi @21:31:34.31 SS.reconfigure :inhaling 0.47ml/s @21:31:34.36 SS.seek 25ml @21:31:42.55 Stroke completed -- waiting for pressure to equalize ... @21:31:42.56 SS.reconfigure :exhale @21:31:42.62 SS.hold @21:32:01.26 Intake.select :close @21:32:01.42 SSV.dial :exhaust @21:32:02.18 Exhaust.select :open @21:32:02.30 SS.seek 0ml @21:32:11.12 Exhaust.select :close @21:32:11.23 SSV.dial CBV @21:32:11.25
CBV.dial SSV @21:32:12.01 Allowing 130s for 25.0ml of remaining 350.0ml @21:32:12.02 Intake.select :open @21:32:14.19 Intake @16.1psia, vacuum from 10.0 to 13.0psi @21:32:14.21 SS.reconfigure :inhaling 0.47ml/s @21:32:14.25 SS.seek 25ml @21:33:10.14 Stroke completed -- waiting for pressure to equalize ... @21:33:10.15 SS.reconfigure :exhale @21:33:10.20 SS.hold @21:33:39.01 Intake.select :close @21:33:39.17 SSV.dial :exhaust @21:33:39.93 Exhaust.select :open @21:33:40.04 SS.seek 0ml @21:33:49.24 Exhaust.select :close @21:33:49.35 SSV.dial CBV @21:33:49.37
CBV.dial SSV @21:33:50.15 Allowing 130s for 25.0ml of remaining 325.0ml @21:33:50.16 Intake.select :open @21:33:52.32 Intake @16.1psia, vacuum from 10.0 to 13.0psi @21:33:52.34 SS.reconfigure :inhaling 0.47ml/s @21:33:52.39 SS.seek 25ml @21:34:47.00 Stroke completed -- waiting for pressure to equalize ... @21:34:47.01 SS.reconfigure :exhale @21:34:47.06 SS.hold @21:35:17.88 Intake.select :close @21:35:18.04 SSV.dial :exhaust @21:35:18.80 Exhaust.select :open @21:35:18.92 SS.seek 0ml @21:35:27.71 Exhaust.select :close @21:35:27.81 SSV.dial CBV @21:35:27.83
CBV.dial SSV @21:35:28.61 Allowing 130s for 25.0ml of remaining 300.0ml @21:35:28.63 Intake.select :open @21:35:30.79 Intake @16.4psia, vacuum from 10.0 to 13.0psi @21:35:30.81 SS.reconfigure :inhaling 0.47ml/s @21:35:30.86 SS.seek 25ml @21:36:21.01 Vacuum too high -- waiting up to 40 seconds @21:36:21.02 SS.reconfigure :exhale @21:36:21.07 SS.hold @21:36:36.03 Intake @18.2psia, vacuum from 10.0 to 13.0psi @21:36:36.04 SS.reconfigure :inhaling 0.38ml/s @21:36:36.09 SS.seek 25ml @21:36:43.12 Stroke completed -- waiting for pressure to equalize ... @21:36:43.14 SS.reconfigure :exhale @21:36:43.18 SS.hold @21:37:01.99 Intake.select :close @21:37:02.15 SSV.dial :exhaust @21:37:02.96 Exhaust.select :open @21:37:03.07 SS.seek 0ml @21:37:11.92 Exhaust.select :close @21:37:12.03 SSV.dial CBV @21:37:12.06
CBV.dial SSV @21:37:12.83 Allowing 130s for 25.0ml of remaining 275.0ml @21:37:12.84 Intake.select :open @21:37:15.01 Intake @16.2psia, vacuum from 10.0 to 13.0psi @21:37:15.03 SS.reconfigure :inhaling 0.38ml/s @21:37:15.07 SS.seek 25ml @21:38:23.75 Stroke completed -- waiting for pressure to equalize ... @21:38:23.76 SS.reconfigure :exhale @21:38:23.81 SS.hold @21:38:52.87 Intake.select :close @21:38:53.03 SSV.dial :exhaust @21:38:53.78 Exhaust.select :open @21:38:53.90 SS.seek 0ml @21:39:02.70 Exhaust.select :close @21:39:02.81 SSV.dial CBV @21:39:02.83
CBV.dial SSV @21:39:03.61 Allowing 130s for 25.0ml of remaining 250.0ml @21:39:03.62 Intake.select :open @21:39:05.79 Intake @16.3psia, vacuum from 10.0 to 13.0psi @21:39:05.81 SS.reconfigure :inhaling 0.38ml/s @21:39:05.86 SS.seek 25ml @21:40:14.67 Stroke completed -- waiting for pressure to equalize ... @21:40:14.68 SS.reconfigure :exhale @21:40:14.73 SS.hold @21:40:47.75 Intake.select :close @21:40:47.91 SSV.dial :exhaust @21:40:48.69 Exhaust.select :open @21:40:48.81 SS.seek 0ml @21:40:57.65 Exhaust.select :close @21:40:57.76 SSV.dial CBV @21:40:57.79
CBV.dial SSV @21:40:58.56 Allowing 130s for 25.0ml of remaining 225.0ml @21:40:58.57 Intake.select :open @21:41:00.74 Intake @16.3psia, vacuum from 10.0 to 13.0psi @21:41:00.76 SS.reconfigure :inhaling 0.38ml/s @21:41:00.80 SS.seek 25ml @21:42:09.87 Stroke completed -- waiting for pressure to equalize ... @21:42:09.88 SS.reconfigure :exhale @21:42:09.93 SS.hold @21:42:44.74 Intake.select :close @21:42:45.82 SSV.dial :exhaust @21:42:46.57 Exhaust.select :open @21:42:46.69 SS.seek 0ml @21:42:55.43 Exhaust.select :close @21:42:55.54 SSV.dial CBV @21:42:55.56
CBV.dial SSV @21:42:56.34 Allowing 130s for 25.0ml of remaining 200.0ml @21:42:56.35 Intake.select :open @21:42:58.52 Intake @16.3psia, vacuum from 10.0 to 13.0psi @21:42:58.54 SS.reconfigure :inhaling 0.38ml/s @21:42:58.59 SS.seek 25ml @21:44:07.80 Stroke completed -- waiting for pressure to equalize ... @21:44:07.81 SS.reconfigure :exhale @21:44:07.86 SS.hold @21:44:46.67 Intake.select :close @21:44:46.83 SSV.dial :exhaust @21:44:47.59 Exhaust.select :open @21:44:47.71 SS.seek 0ml @21:44:56.55 Exhaust.select :close @21:44:56.66 SSV.dial CBV @21:44:56.69
CBV.dial SSV @21:44:57.46 Allowing 130s for 25.0ml of remaining 175.0ml @21:44:57.47 Intake.select :open @21:44:59.64 Intake @16.4psia, vacuum from 10.0 to 13.0psi @21:44:59.66 SS.reconfigure :inhaling 0.38ml/s @21:44:59.71 SS.seek 25ml @21:45:59.28 Vacuum too high -- waiting up to 40 seconds @21:45:59.29 SS.reconfigure :exhale @21:45:59.34 SS.hold @21:46:16.13 Intake @17.4psia, vacuum from 10.0 to 13.0psi @21:46:16.15 SS.reconfigure :inhaling 0.31ml/s @21:46:16.20 SS.seek 25ml @21:46:28.77 Stroke completed -- waiting for pressure to equalize ... @21:46:28.78 SS.reconfigure :exhale @21:46:28.83 SS.hold @21:46:55.37 Intake.select :close @21:46:55.53 SSV.dial :exhaust @21:46:56.31 Exhaust.select :open @21:46:56.43 SS.seek 0ml @21:47:05.46 Exhaust.select :close @21:47:05.56 SSV.dial CBV @21:47:05.59
CBV.dial SSV @21:47:06.35 Allowing 130s for 25.0ml of remaining 150.0ml @21:47:06.36 Intake.select :open @21:47:08.53 Intake @16.4psia, vacuum from 10.0 to 13.0psi @21:47:08.55 SS.reconfigure :inhaling 0.31ml/s @21:47:08.60 SS.seek 25ml @21:48:35.98 Stroke completed -- waiting for pressure to equalize ... @21:48:36.00 SS.reconfigure :exhale @21:48:36.04 SS.hold @21:49:14.86 Intake.select :close @21:49:15.01 SSV.dial :exhaust @21:49:15.78 Exhaust.select :open @21:49:15.89 SS.seek 0ml @21:49:24.95 Exhaust.select :close @21:49:25.06 SSV.dial CBV @21:49:25.09
CBV.dial SSV @21:49:25.86 Allowing 130s for 25.0ml of remaining 125.0ml @21:49:25.87 Intake.select :open @21:49:28.04 Intake @16.3psia, vacuum from 10.0 to 13.0psi @21:49:28.05 SS.reconfigure :inhaling 0.31ml/s @21:49:28.10 SS.seek 25ml @21:50:57.17 Stroke completed -- waiting for pressure to equalize ... @21:50:57.18 SS.reconfigure :exhale @21:50:57.23 SS.hold @21:51:35.88 SS.reconfigure :default @21:51:35.93 SS.hold @21:51:36.00 Intake.select :close @21:51:36.16 ShallowSampler::Clogged in MAIN -- Filtering too Slowly after 900.0ml @21:51:36.17 SSV.dial :exhaust @21:51:36.95 Exhaust.select :open @21:51:37.07 SS.reconfigure :exhale @21:51:37.11 SS.seek 0ml @21:51:45.98 Exhaust.select :close @21:51:46.10 Sampled 900.0ml @21:51:46.11 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @21:51:46.12 Intake.select :close @21:51:46.18 CSV.dial :waste @21:51:46.34 SSV.dial CBV,avoiding: :exhaust @21:51:46.36 CS.seek 0ml @21:51:46.40
CBV.dial SSV @21:51:46.94 CSV.dial :air @21:51:47.68 CS.seek 10ml @21:51:48.77
CTV.dial :air @21:51:49.55 delay 15 seconds @21:52:04.57 SSV.dial :spit,avoiding: :exhaust @21:52:05.90 CTV.dial CSV,avoiding: :intake @21:52:05.93
SS.seek 0ml @21:52:06.05 SSV.dial CBV,avoiding: :exhaust @21:52:12.19 SSV.dial between CBV and plug @21:52:12.68 CBV.dial between SSV and waste2 @21:52:13.06 CSV.dial between air and CTV @21:52:13.38 CS.seek 8.255ml @21:52:18.22 CTV.dial CSV,avoiding: :air @21:52:18.24
CSV.dial CTV @21:52:18.58 CS.reconfigure :pressurize @21:52:18.63 CS.seek 0ml @21:52:32.26 BubblePoint: Intake @24.2psia, Outlet @1.0psia @21:52:32.27 CBV.dial SSV @21:52:32.63 SS.reconfigure :default @21:52:32.68 SS.seek 10ml @21:52:38.25 delay 15 seconds @21:52:53.38 Evacuated: Intake @23.7psia, Outlet @23.3psia @21:52:53.39 SSV.dial :spit,avoiding: :exhaust @21:52:54.44 SS.reconfigure :exhale @21:52:54.49 SS.seek 0ml @21:52:59.44 SS.reconfigure :fast @21:52:59.49 SS.seek 5ml @21:53:02.30 SSV.dial CBV,avoiding: :exhaust @21:53:02.32
CBV.dial SSV @21:53:03.67 CTV.dial :air,avoiding: :intake @21:53:05.01 CSV.dial CTV @21:53:05.07
SS.seek 10ml @21:53:06.18 CSV.dial :air @21:53:06.64 CS.reconfigure :fast @21:53:06.69 CS.seek 0ml @21:53:07.58
SSV.dial :spit,avoiding: :exhaust @21:53:08.89 SS.reconfigure :exhale @21:53:08.94 SS.seek 0ml @21:53:13.96 SS.reconfigure :fast @21:53:14.01 SS.seek 5ml @21:53:14.68 CS.stop @21:53:16.81 SSV.dial CBV,avoiding: :exhaust @21:53:16.84
CBV.dial SSV @21:53:19.26 SSV.dial :spit,avoiding: :exhaust @21:53:20.58 SS.reconfigure :default @21:53:20.63 SS.seek 0ml @21:53:23.89 SS.stop @21:53:23.93 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @21:53:23.94 Intake.select :close @21:53:24.01 CSV.dial :waste @21:53:24.15 SSV.dial CBV,avoiding: :exhaust @21:53:24.17
CBV.dial SSV @21:53:24.81 CS.seek 0ml @21:53:24.88 CSV.dial :air @21:53:25.55
CTV.dial :air @21:53:25.63 CS.seek 10ml @21:53:25.72
delay 15 seconds @21:53:40.73 SSV.dial :spit,avoiding: :exhaust @21:53:42.07 CTV.dial CSV,avoiding: :intake @21:53:42.10
SS.reconfigure :exhale @21:53:42.20 SS.seek 0ml @21:53:42.62 SSV.dial CBV,avoiding: :exhaust @21:53:50.12 SSV.dial between CBV and plug @21:53:50.61 CBV.dial between SSV and waste2 @21:53:51.01 CSV.dial between air and CTV @21:53:51.33 CS.seek 7.479ml @21:53:57.97 CTV.dial CSV,avoiding: :air @21:53:57.99
CSV.dial CTV @21:53:58.35 CS.reconfigure :pressurize @21:53:58.40 CS.seek 0ml @21:54:11.57 BubblePoint: Intake @24.2psia, Outlet @3.5psia @21:54:11.58 CBV.dial SSV @21:54:11.91 SS.reconfigure :default @21:54:11.96 SS.seek 10ml @21:54:17.53 delay 15 seconds @21:54:32.66 Evacuated: Intake @23.9psia, Outlet @23.6psia @21:54:32.67 SSV.dial :spit,avoiding: :exhaust @21:54:33.70 SS.reconfigure :exhale @21:54:33.75 SS.seek 0ml @21:54:38.72 SS.reconfigure :fast @21:54:38.77 SS.seek 5ml @21:54:41.61 SSV.dial CBV,avoiding: :exhaust @21:54:41.63
CBV.dial SSV @21:54:42.98 CTV.dial :air,avoiding: :intake @21:54:44.31 CSV.dial CTV @21:54:44.34
SS.seek 10ml @21:54:45.48 CSV.dial :air @21:54:46.37 CS.reconfigure :fast @21:54:46.43 CS.seek 0ml @21:54:46.81
SSV.dial :spit,avoiding: :exhaust @21:54:48.14 SS.reconfigure :exhale @21:54:48.19 SS.seek 0ml @21:54:53.05 CS.stop @21:54:53.19
SS.reconfigure :fast @21:54:53.24 SS.seek 5ml @21:54:56.08 SSV.dial CBV,avoiding: :exhaust @21:54:56.10
CBV.dial SSV @21:54:58.46 SSV.dial :spit,avoiding: :exhaust @21:54:59.77 SS.reconfigure :default @21:54:59.82 SS.seek 0ml @21:55:03.06 SS.stop @21:55:03.10 Lysing: Adding 2.0ml AqMeOH @21:55:03.11 CBV.dial between CSV and waste1,avoiding: :DBV @21:55:04.62 CTV.dial CSV @21:55:05.92 CSV.dial CSR @21:55:06.71 Pulling 1ml of CSR.AqMeOH @21:55:06.72 CSR.select :AqMeOH @21:55:06.83 CS.reconfigure :default @21:55:06.88 CS.seek 1ml @21:55:10.28 delay 5 seconds @21:55:15.29 CSR.select :air @21:55:15.47 CS.seek 6ml @21:55:29.15 delay 2 seconds @21:55:31.17 CSV.dial :waste2 @21:55:31.62 CS.reconfigure :fast @21:55:31.67 CS.seek 0ml @21:55:45.99 CSV.dial CSR @21:55:46.45 Pulling 2ml of CSR.AqMeOH @21:55:46.46 CSR.select :AqMeOH @21:55:46.58 CS.reconfigure :default @21:55:46.63 CS.seek 2ml @21:55:52.59 delay 5 seconds @21:55:57.61 CSR.select :air @21:55:57.77 CS.seek 5ml @21:56:06.24 delay 2 seconds @21:56:08.26 CSV.dial CTV @21:56:08.72 CS.seek 1ml @21:56:20.10 Before lysing Collection Puck Heater at 14C/31.0psia @21:56:20.12 Heating Puck.from 4, :sh1 in Collection Clamp to 70C for 10:00 @21:56:20.14 CH.seek 70C,8:00 @21:58:30.89 CH.hold 10:00,70C @21:58:31.08 Begun lysing Collection Puck Heater at 68C/38.0psia @22:08:31.09 Ended lysing Collection Puck Heater at 70C/36.6psia @22:08:31.11 CS.seek 0ml @22:08:34.74 CTV.dial between spare and air,avoiding: :intake @22:08:35.78 CSV.dial :air @22:08:36.23 CS.seek 5ml @22:08:50.01 CSV.dial CTV @22:08:50.47 delay 2 seconds @22:08:52.48 CSV.dial :air @22:08:52.93 delay 2 seconds @22:08:54.95 CSV.dial CTV @22:08:55.41 delay 2 seconds @22:08:57.43 CSV.dial :air @22:08:57.87 delay 2 seconds @22:08:59.89 CS.seek 0ml @22:09:13.57 Puck @82.2psia after final lysis pressurization @22:09:13.58 Recovering Lysate into Collection syringe @22:09:13.59 CSV.dial CBV @22:09:14.60 CBV.dial CSV @22:09:14.98 CS.seek 5ml @22:09:28.76 delay 5 seconds @22:09:33.78 CTV.dial :air @22:09:34.29 CS.seek 7ml @22:09:40.20 delay 5 seconds @22:09:45.22 CSV.dial :air2 @22:09:45.66 CS.seek 10ml @22:09:54.23 CSV.dial between air and CTV,avoiding: CBV @22:09:55.39 Lysing Complete @22:09:55.46 Storing sh1 puck in Collection Clamp to tube 3 @22:09:55.64 SC.reconfigure :tight @22:09:55.68 SC.seek 3 @22:09:59.26 SC.configure :limp @22:09:59.48 Elbow.reconfigure :tight @22:09:59.53 Elbow.seek 3 @22:10:01.28 Forearm.reconfigure :tight @22:10:01.33 Forearm.seek 3 + 450 counts @22:10:02.34 SE.reconfigure :pick @22:10:02.39 SE.seek :beamBreak @22:10:03.85 SE.configure :default @22:10:04.01 Elbow.reconfigure :limp @22:10:04.50 Forearm.reconfigure :loose @22:10:04.56 Forearm.seek 3 @22:10:04.76 Elbow.hold @22:10:04.83 Elbow.reconfigure :tight @22:10:04.93 SE.seek 25.12 Pucks below Top Plate @22:10:05.43 Forearm.force -20 @22:10:05.48 Forearm.seek 3 + 316 counts @22:10:05.77 Forearm.reconfigure :tight @22:10:05.82 Forearm.seek 3 + 450 counts @22:10:06.02 SE.seek 26.12 Pucks below Top Plate @22:10:06.70 Forearm.reconfigure :loose @22:10:06.75 Forearm.seek :retract @22:10:07.67 Elbow.seek CC @22:10:09.30 Elbow.reconfigure :loose @22:10:09.40 Forearm.seek CC @22:10:10.35 Hand.close @22:10:11.01 CC.seek :open @22:10:15.76 Forearm.seek :retract @22:10:16.73 Elbow.reconfigure :tight @22:10:16.78 Elbow.seek 3 @22:10:18.55 Elbow.reconfigure :limp @22:10:18.60 Forearm.seek 3 @22:10:19.63 Elbow.hold @22:10:19.70 Elbow.reconfigure :tight @22:10:19.75 Hand.open @22:10:20.51 Forearm.force -20 @22:10:20.56 Forearm.seek 3 + 311 counts @22:10:20.85 Forearm.reconfigure :tight @22:10:20.90 Forearm.seek 3 + 450 counts @22:10:21.59 SE.seek :down @22:10:22.53 SE.stop @22:10:22.73
Forearm.reconfigure :loose @22:10:22.77 Forearm.seek :retract @22:10:23.71 Tranferring DA @22:10:23.73 Beginning samplePassC @22:10:23.74 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @22:10:23.93 Elbow.seek FlushPuck::Garage @22:10:25.84 Elbow.reconfigure :loose @22:10:25.94 Forearm.seek :garage @22:10:26.96 Hand.close @22:10:27.75 Forearm.seek :retract @22:10:28.72 Elbow.reconfigure :tight @22:10:28.76 Elbow.seek CC @22:10:31.74 Elbow.reconfigure :loose @22:10:31.84 Forearm.reconfigure :tight @22:10:31.89 Forearm.seek CC @22:10:33.10 CC.seek :closed @22:10:37.61 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.40 @22:10:37.68 Hand.open @22:10:38.32 Forearm.reconfigure :loose @22:10:38.37 Forearm.seek :retract @22:10:39.38 PSV.dial PSR @22:10:40.90 Pulling 1.5ml of PSR.flush @22:10:41.34 PSR.select :flush @22:10:41.48 PS.seek 1.5ml @22:10:57.58 delay 5 seconds @22:11:02.60 PSV.dial :waste1 @22:11:03.08 PS.seek 0ml @22:11:19.29 PSV.dial PSR @22:11:19.76 PSR.select :air @22:11:19.88 PS.seek 2.5ml @22:11:46.30 PRV.dial CBV,avoiding: :ab2 @22:11:46.32
CBV.dial PRV @22:11:48.09 PSV.dial :mixloop @22:11:49.12 CTV.dial :air,avoiding: :intake @22:11:49.20 PTV.dial :PRVmixing @22:11:50.52 PS.seek 0ml @22:12:17.04 PSV.dial :air @22:12:17.52 PS.seek 2.5ml @22:12:43.85 PSV.dial :mixloop @22:12:44.33 PS.seek 0ml @22:13:10.85 PSV.dial :air @22:13:11.33 PS.seek 2.5ml @22:13:37.70 PSV.dial :mixloop @22:13:38.17 PS.seek 0ml @22:14:04.51 Garaging FlushPuck in Collection Clamp @22:14:04.58 Elbow.reconfigure :tight @22:14:04.63 Elbow.seek CC @22:14:04.95 Elbow.reconfigure :loose @22:14:05.05 Forearm.seek CC @22:14:06.05 Hand.close @22:14:06.63 CC.seek :open @22:14:11.25 Forearm.seek :retract @22:14:12.31 Elbow.reconfigure :tight @22:14:12.35 Elbow.seek :garage @22:14:15.24 Elbow.reconfigure :loose @22:14:15.34 Forearm.reconfigure :tight @22:14:15.39 Forearm.seek :garage @22:14:16.44 Hand.open @22:14:17.50 Forearm.reconfigure :loose @22:14:17.55 Forearm.seek :retract @22:14:18.52 PSV.dial :mixloop @22:14:18.60 PTV.dial :PRVmixing @22:14:18.68 PRV.dial CBV @22:14:18.72
CBV.dial PRV @22:14:18.80 CTV.dial :air,avoiding: :intake @22:14:18.89 Loading lyfil puck from tube 4 into Collection Clamp @22:14:19.13 SC.reconfigure :tight @22:14:19.18 SC.seek 4 @22:14:22.75 SC.configure :limp @22:14:22.97 Elbow.reconfigure :tight @22:14:23.02 Elbow.seek 4 @22:14:24.97 Forearm.reconfigure :tight @22:14:25.02 Forearm.seek 4 + 450 counts @22:14:26.16 SE.reconfigure :pick @22:14:26.22 SE.seek :beamBreak @22:14:28.99 SE.configure :default @22:14:29.15 Elbow.seek 4 @22:14:29.33 Forearm.seek 4 + 300 counts @22:14:29.57 Forearm.reconfigure :loose @22:14:29.63 Hand.close @22:14:30.27 SE.seek :down @22:14:30.47
Forearm.seek :retract @22:14:31.49 Elbow.seek CC @22:14:32.77 SE.stop @22:14:33.16
Elbow.reconfigure :loose @22:14:33.26 Forearm.reconfigure :tight @22:14:33.31 Forearm.seek CC @22:14:34.62 CC.seek :closed @22:14:39.14 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.15 @22:14:39.15 Hand.open @22:14:39.83 Forearm.reconfigure :loose @22:14:39.88 Forearm.seek :retract @22:14:40.90 CTV.dial CSV,avoiding: :intake @22:14:42.24 CSV.dial CTV @22:14:42.57 PS.seek 2.5ml @22:15:09.07 CS.seek 4.501ml @22:15:23.94 delay 8 seconds @22:15:31.96 PSV.dial :air @22:15:32.43 delay 2 seconds @22:15:34.45 PSV.dial :waste2 @22:15:35.19 PS.seek 0ml @22:16:01.51 CBV.dial between PRV and waste1 @22:16:01.83 CSV.dial :air @22:16:02.31 CS.seek 10ml @22:16:17.19 CSV.dial CTV @22:16:17.65 CS.seek 0ml @22:16:44.03 CBV.dial :waste1 @22:16:44.35 delay 5 seconds @22:16:49.37 CTV.dial :air,avoiding: :intake @22:16:50.76 Storing lyfil puck in Collection Clamp to tube 3 @22:16:50.94 SC.reconfigure :tight @22:16:50.99 SC.seek 3 @22:16:54.58 SC.configure :limp @22:16:54.80 Elbow.reconfigure :tight @22:16:54.85 Elbow.seek 3 @22:16:57.10 Forearm.reconfigure :tight @22:16:57.15 Forearm.seek 3 + 450 counts @22:16:58.19 SE.reconfigure :pick @22:16:58.25 SE.seek :beamBreak @22:16:59.68 SE.configure :default @22:16:59.85 Elbow.reconfigure :limp @22:16:59.90 Forearm.reconfigure :loose @22:16:59.95 Forearm.seek 3 @22:17:00.22 Elbow.hold @22:17:00.29 Elbow.reconfigure :tight @22:17:00.39 SE.seek 26.12 Pucks below Top Plate @22:17:00.86 Forearm.force -20 @22:17:00.91 Forearm.seek 3 + 295 counts @22:17:01.18 Forearm.reconfigure :tight @22:17:01.23 Forearm.seek 3 + 450 counts @22:17:01.43 SE.seek 27.12 Pucks below Top Plate @22:17:01.99 Forearm.reconfigure :loose @22:17:02.04 Forearm.seek :retract @22:17:02.97 Elbow.seek CC @22:17:04.62 Elbow.reconfigure :loose @22:17:04.73 Forearm.seek CC @22:17:05.74 Hand.close @22:17:06.38 CC.seek :open @22:17:11.12 Forearm.seek :retract @22:17:12.15 Elbow.reconfigure :tight @22:17:12.20 Elbow.seek 3 @22:17:14.42 Elbow.reconfigure :limp @22:17:14.48 Forearm.seek 3 @22:17:15.53 Elbow.hold @22:17:15.60 Elbow.reconfigure :tight @22:17:15.65 Hand.open @22:17:16.39 Forearm.force -20 @22:17:16.43 Forearm.seek 3 + 316 counts @22:17:16.72 Forearm.reconfigure :tight @22:17:16.77 Forearm.seek 3 + 450 counts @22:17:17.03 SE.seek :down @22:17:17.90 SE.stop @22:17:18.16
Forearm.reconfigure :loose @22:17:18.21 Forearm.seek :retract @22:17:19.14 PSV.dial :air @22:17:19.90 PS.seek 2.5ml @22:17:46.35 delay 1 second @22:17:47.36 PSV.dial :mixloop @22:17:47.82 delay 1 second @22:17:48.83 PSV.dial :air @22:17:49.43 delay 1 second @22:17:50.44 PSV.dial :mixloop @22:17:50.90 delay 1 second @22:17:51.91 PSV.dial :air @22:17:52.38 delay 1 second @22:17:53.40 PS.seek 0ml @22:18:19.79 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @22:18:19.98 Elbow.seek FlushPuck::Garage @22:18:21.83 Elbow.reconfigure :loose @22:18:21.93 Forearm.seek :garage @22:18:22.92 Hand.close @22:18:23.62 Forearm.seek :retract @22:18:24.60 Elbow.reconfigure :tight @22:18:24.65 Elbow.seek PC @22:18:26.36 Elbow.reconfigure :loose @22:18:26.46 Forearm.reconfigure :tight @22:18:26.51 Forearm.seek PC @22:18:27.82 PC.seek :closed @22:18:32.45 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.63 @22:18:32.52 Hand.open @22:18:33.19 Forearm.reconfigure :loose @22:18:33.24 Forearm.seek :retract @22:18:34.24 PRV.dial :air @22:18:34.69 PSV.dial :mixloop @22:18:35.23 PS.seek 0.3ml @22:18:39.10 PSV.dial PSR @22:18:40.28 Pulling 0.4ml of PSR.air @22:18:40.29 PSR.select :air @22:18:40.31 PS.seek 0.7ml @22:18:45.32 delay 3 seconds @22:18:48.34 PSR.select :air @22:18:48.36 PS.seek 2.5ml @22:19:07.63 delay 3 seconds @22:19:10.65 PSV.dial :mixloop @22:19:11.67 PTV.dial :mixing @22:19:12.44 PBV.dial :waste2 @22:19:13.40 PS.seek 0ml @22:19:39.72 Garaging FlushPuck in Processing Clamp @22:19:39.80 Elbow.reconfigure :tight @22:19:39.84 Elbow.seek PC @22:19:39.97 Elbow.reconfigure :loose @22:19:40.07 Forearm.seek PC @22:19:41.00 Hand.close @22:19:41.63 PC.seek :open @22:19:46.79 Forearm.seek :retract @22:19:47.82 Elbow.reconfigure :tight @22:19:47.87 Elbow.seek :garage @22:19:49.23 Elbow.reconfigure :loose @22:19:49.33 Forearm.reconfigure :tight @22:19:49.38 Forearm.seek :garage @22:19:50.41 Hand.open @22:19:51.06 Forearm.reconfigure :loose @22:19:51.11 Forearm.seek :retract @22:19:52.10 Loading da puck from tube 4 into Processing Clamp @22:19:52.34 SC.reconfigure :tight @22:19:52.39 SC.seek 4 @22:19:55.96 SC.configure :limp @22:19:56.18 Elbow.reconfigure :tight @22:19:56.23 Elbow.seek 4 @22:19:58.10 Forearm.reconfigure :tight @22:19:58.15 Forearm.seek 4 + 450 counts @22:19:59.17 SE.reconfigure :pick @22:19:59.27 SE.seek :beamBreak @22:20:02.19 SE.configure :default @22:20:02.35 Elbow.seek 4 @22:20:02.70 Forearm.seek 4 + 300 counts @22:20:02.99 Forearm.reconfigure :loose @22:20:03.04 Hand.close @22:20:03.64 SE.seek :down @22:20:03.84
Forearm.seek :retract @22:20:04.82 Elbow.seek PC @22:20:06.14 SE.stop @22:20:07.32
Elbow.reconfigure :loose @22:20:07.80 Forearm.reconfigure :tight @22:20:07.92 Forearm.seek PC @22:20:09.11 PC.seek :closed @22:20:13.78 Processing Clamp onSpring/offSpring for 16/26 samples, on/off torque 1.62 @22:20:13.80 Hand.open @22:20:14.44 Forearm.reconfigure :loose @22:20:14.49 Forearm.seek :retract @22:20:15.49 Heating Puck.from 4, :da in Processing Clamp to 28C for 2:00:00 @22:20:15.51 PH.seek 28C,5:00 @22:20:56.14 PH.hold 2:00:00,28C @22:20:56.19 delay 1:30 @22:22:26.24 PSR.select :air @22:22:26.26 PSV.dial PSR @22:22:27.31 Pulling 0.5ml of PSR.wash @22:22:27.32 PSR.select :wash @22:22:27.45 PS.seek 0.5ml @22:22:33.37 delay 5 seconds @22:22:38.39 PSR.select :air @22:22:38.55 PS.seek 2ml @22:22:54.81 PTV.dial :puck @22:22:55.56 PSV.dial PTV @22:22:56.33 PBV.dial :waste1 @22:22:57.72 PS.seek 1.5ml @22:23:03.66 delay 3 seconds @22:23:06.67 PBV.dial between PSV and waste1 @22:23:07.04 PS.seek 0ml @22:23:23.19 delay 5:00 @22:28:23.21 recoverPSRreagent08 @22:28:23.22 PTV.dial :puck @22:28:23.40 PBV.dial :waste1 @22:28:23.78 delay 30 seconds @22:28:53.80 PBV.dial between PSV and waste1 @22:28:54.18 PSR.select :air @22:28:54.20 PSV.dial PSR @22:28:54.97 PS.reconfigure :fast @22:28:55.02 PS.seek 2.5ml @22:29:18.83 PSV.dial PTV @22:29:19.62 delay 5 seconds @22:29:24.64 PSV.dial PSR @22:29:25.40 delay 5 seconds @22:29:30.42 PSV.dial PTV @22:29:31.18 delay 5 seconds @22:29:36.20 PSV.dial :air @22:29:37.08 PS.seek 0ml @22:30:00.18 PTV.dial :PRVmixing,via: PRV @22:30:01.52 PSV.dial :mixloop @22:30:01.97 PRV.dial :ab1 @22:30:02.73 PS.reconfigure :slow @22:30:02.78 PS.seek 0.15ml @22:30:06.37 delay 2 seconds @22:30:08.39 CBV.dial PRV @22:30:08.43
PRV.dial CBV @22:30:08.98 PS.seek 0.35ml @22:30:13.62 delay 2 seconds @22:30:15.63 CBV.dial between PRV and PSV @22:30:16.39 PRV.dial :air @22:30:16.86 PS.seek 0.9ml @22:30:28.65 PRV.dial :waste2 @22:30:29.40 PS.seek 0.5ml @22:30:38.12 addPMLReagent08 @22:30:38.13 PSV.dial :air @22:30:38.61 PS.reconfigure :default @22:30:38.65 PS.seek 2ml @22:30:54.86 PSV.dial :PML @22:30:55.33 PTV.dial :mixing @22:30:56.10 PBV.dial :waste1 @22:30:56.54 PS.seek 1.85ml @22:30:58.92 PBV.dial between PSV and waste1 @22:30:59.29 PS.seek 0ml @22:31:19.05 delay 2 seconds @22:31:21.07 Beginning samplePassC @22:31:21.08 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @22:31:21.27 Elbow.reconfigure :tight @22:31:21.32 Elbow.seek FlushPuck::Garage @22:31:22.68 Elbow.reconfigure :loose @22:31:22.78 Forearm.seek :garage @22:31:23.73 Hand.close @22:31:24.50 Forearm.seek :retract @22:31:25.53 Elbow.reconfigure :tight @22:31:25.58 Elbow.seek CC @22:31:28.61 Elbow.reconfigure :loose @22:31:28.71 Forearm.reconfigure :tight @22:31:28.76 Forearm.seek CC @22:31:29.98 CC.seek :closed @22:31:34.51 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.44 @22:31:34.59 Hand.open @22:31:35.31 Forearm.reconfigure :loose @22:31:35.36 Forearm.seek :retract @22:31:36.30 PSV.dial PSR @22:31:37.36 Pulling 1.5ml of PSR.flush @22:31:37.37 PSR.select :flush @22:31:37.50 PS.seek 1.5ml @22:31:53.68 delay 5 seconds @22:31:58.70 PSV.dial :waste1 @22:31:59.17 PS.seek 0ml @22:32:15.27 PSV.dial PSR @22:32:15.74 PSR.select :air @22:32:15.86 PS.seek 2.5ml @22:32:42.18 PRV.dial CBV,avoiding: :ab2 @22:32:42.20
CBV.dial PRV @22:32:43.83 PSV.dial :mixloop @22:32:44.88 CTV.dial :air,avoiding: :intake @22:32:44.96 PTV.dial :PRVmixing @22:32:45.73 PS.seek 0ml @22:33:12.20 PSV.dial :air @22:33:12.68 PS.seek 2.5ml @22:33:39.08 PSV.dial :mixloop @22:33:39.56 PS.seek 0ml @22:34:05.92 PSV.dial :air @22:34:06.40 PS.seek 2.5ml @22:34:32.95 PSV.dial :mixloop @22:34:33.43 PS.seek 0ml @22:34:59.96 Garaging FlushPuck in Collection Clamp @22:35:00.03 Elbow.reconfigure :tight @22:35:00.08 Elbow.seek CC @22:35:00.20 Elbow.reconfigure :loose @22:35:00.30 Forearm.seek CC @22:35:01.30 Hand.close @22:35:01.87 CC.seek :open @22:35:06.51 Forearm.seek :retract @22:35:07.49 Elbow.reconfigure :tight @22:35:07.98 Elbow.seek :garage @22:35:10.92 Elbow.reconfigure :loose @22:35:11.02 Forearm.reconfigure :tight @22:35:11.07 Forearm.seek :garage @22:35:12.02 Hand.open @22:35:12.68 Forearm.reconfigure :loose @22:35:12.72 Forearm.seek :retract @22:35:13.70 delayUntil "22:39:21.06" @22:35:13.72 Whole Cell Archive sampling 1000ml @22:35:13.74 Loading wcr puck from tube 4 into Collection Clamp @22:35:14.22 Elbow.reconfigure :tight @22:35:14.27 Elbow.seek 4 @22:35:16.22 Forearm.reconfigure :tight @22:35:16.28 Forearm.seek 4 + 450 counts @22:35:17.36 SE.reconfigure :pick @22:35:17.42 SE.seek :beamBreak @22:35:20.32 SE.configure :default @22:35:20.49 Elbow.seek 4 @22:35:20.68 Forearm.seek 4 + 300 counts @22:35:20.90 Forearm.reconfigure :loose @22:35:20.95 Hand.close @22:35:21.63 SE.seek :down @22:35:21.84 Forearm.seek :retract @22:35:22.84 Elbow.seek CC @22:35:24.20 SE.stop @22:35:24.85 Elbow.reconfigure :loose @22:35:25.02 Forearm.reconfigure :tight @22:35:25.07 Forearm.seek CC @22:35:26.36 CC.seek :closed @22:35:30.89 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.44 @22:35:30.91 Hand.open @22:35:31.55 Forearm.reconfigure :loose @22:35:31.60 Forearm.seek :retract @22:35:32.57 collectSample 1000ml w/bubble point of 35psi @22:35:32.58 Priming 7ml @22:35:32.60 Intake.select :close @22:35:32.61 Exhaust.select :close @22:35:32.62 SS.hold @22:35:32.83 Calibrating pressure sensors @22:35:32.85 CTV.dial :air @22:35:32.93 SSV.dial :spit,avoiding: :exhaust @22:35:33.01 SS.reconfigure :fast @22:35:33.07 SS.seek 5ml @22:35:35.85 SSV.dial CBV @22:35:35.88 CBV.dial SSV @22:35:37.26 delay 3.8 seconds @22:35:41.20 Sensor limits: Intake max 104.0psia; Outlet max 95.7psia @22:35:41.21 SSV.dial :spit @22:35:42.55 SS.seek 0ml @22:35:45.27 SSV.dial CBV @22:35:46.63 CTV.dial :intake @22:35:47.39 SSV.dial CBV @22:35:47.41 CBV.dial SSV @22:35:47.65 Allowing 40s for 7.0ml of remaining 7.0ml @22:35:47.66 Intake.select :open @22:35:49.83 Intake @14.4psia, vacuum from 8.9 to 11.9psi @22:35:50.29 SS.reconfigure :inhaling 2.20ml/s @22:35:50.35 SS.seek 7ml @22:35:57.26 Stroke completed -- waiting for pressure to equalize ... @22:35:57.27 SS.reconfigure :exhale @22:35:57.32 SS.hold @22:36:17.68 Intake.select :close @22:36:17.84 SSV.dial :exhaust @22:36:18.62 Exhaust.select :open @22:36:18.74 SS.seek 0ml @22:36:22.87 Exhaust.select :close @22:36:22.99 Sampling 1000.0ml @22:36:23.00 Intake.select :close @22:36:23.01 Exhaust.select :close @22:36:23.02 SS.reconfigure :default @22:36:23.07 SS.hold @22:36:23.29 CTV.dial :intake @22:36:23.47 SSV.dial CBV @22:36:23.51 CBV.dial SSV @22:36:24.31 Allowing 130s for 25.0ml of remaining 1000.0ml @22:36:24.32 Intake.select :open @22:36:26.49 Intake @15.4psia, vacuum from 9.9 to 12.9psi @22:36:26.51 SS.reconfigure :inhaling 2.20ml/s @22:36:26.56 SS.seek 25ml @22:36:35.84 Vacuum too high -- waiting up to 40 seconds @22:36:35.86 SS.reconfigure :exhale @22:36:35.91 SS.hold @22:36:47.51 Intake @19.7psia, vacuum from 10.0 to 13.0psi @22:36:47.52 SS.reconfigure :inhaling 1.76ml/s @22:36:47.57 SS.seek 25ml @22:36:50.69 Vacuum too high -- waiting up to 40 seconds @22:36:50.70 SS.reconfigure :exhale @22:36:50.76 SS.hold @22:36:53.04 Intake @19.9psia, vacuum from 10.0 to 13.0psi @22:36:53.06 SS.reconfigure :inhaling 1.41ml/s @22:36:53.10 SS.seek 25ml @22:36:55.06 Vacuum too high -- waiting up to 40 seconds @22:36:55.08 SS.reconfigure :exhale @22:36:55.13 SS.hold @22:36:56.56 Intake @18.7psia, vacuum from 10.0 to 13.0psi @22:36:56.58 SS.reconfigure :inhaling 1.13ml/s @22:36:56.63 SS.seek 25ml @22:36:59.78 Vacuum too high -- waiting up to 40 seconds @22:36:59.80 SS.reconfigure :exhale @22:36:59.85 SS.hold @22:37:02.45 Intake @20.0psia, vacuum from 10.0 to 13.0psi @22:37:02.47 SS.reconfigure :inhaling 0.91ml/s @22:37:02.52 SS.seek 25ml @22:37:05.29 Vacuum too high -- waiting up to 40 seconds @22:37:05.31 SS.reconfigure :exhale @22:37:05.81 SS.hold @22:37:06.26 Intake @18.5psia, vacuum from 10.0 to 13.0psi @22:37:06.28 SS.reconfigure :inhaling 0.73ml/s @22:37:06.33 SS.seek 25ml @22:37:09.55 Stroke completed -- waiting for pressure to equalize ... @22:37:09.56 SS.reconfigure :exhale @22:37:09.61 SS.hold @22:37:44.42 Intake.select :close @22:37:44.58 SSV.dial :exhaust @22:37:45.35 Exhaust.select :open @22:37:45.47 SS.seek 0ml @22:37:54.24 Exhaust.select :close @22:37:54.35 SSV.dial CBV @22:37:54.38 CBV.dial SSV @22:37:55.16 Allowing 130s for 25.0ml of remaining 975.0ml @22:37:55.18 Intake.select :open @22:37:57.35 Intake @15.8psia, vacuum from 10.0 to 13.0psi @22:37:57.37 SS.reconfigure :inhaling 0.73ml/s @22:37:57.42 SS.seek 25ml @22:38:34.16 Stroke completed -- waiting for pressure to equalize ... @22:38:34.17 SS.reconfigure :exhale @22:38:34.22 SS.hold @22:39:09.47 Intake.select :close @22:39:09.63 SSV.dial :exhaust @22:39:10.41 Exhaust.select :open @22:39:10.53 SS.seek 0ml @22:39:19.66 Exhaust.select :close @22:39:19.77 SSV.dial CBV @22:39:19.82 CBV.dial SSV @22:39:20.57 Allowing 130s for 25.0ml of remaining 950.0ml @22:39:20.58 Intake.select :open @22:39:21.07 recoverPRVreagent08 @22:39:21.08 PTV.dial :mixing @22:39:21.84 PBV.dial :waste1 @22:39:22.22 delay 30 seconds @22:39:22.75 Intake @16.0psia, vacuum from 10.0 to 13.0psi @22:39:22.77 SS.reconfigure :inhaling 0.73ml/s @22:39:22.82 SS.seek 25ml @22:39:52.24 PBV.dial between PSV and waste1 @22:39:52.62 PSV.dial :air @22:39:53.10 PS.reconfigure :fast @22:39:53.15 PS.seek 2.5ml @22:39:59.11 Stroke completed -- waiting for pressure to equalize ... @22:39:59.13 SS.reconfigure :exhale @22:39:59.18 SS.hold @22:40:17.57 PSV.dial :mixloop @22:40:18.04 delay 2 seconds @22:40:20.06 PSV.dial :air @22:40:20.68 delay 2 seconds @22:40:22.69 PSV.dial :mixloop @22:40:23.14 delay 2 seconds @22:40:25.15 PSV.dial :air @22:40:25.63 PS.seek 0ml @22:40:31.95 Intake.select :close @22:40:32.11 SSV.dial :exhaust @22:40:32.88 Exhaust.select :open @22:40:33.00 SS.seek 0ml @22:40:42.05 Exhaust.select :close @22:40:42.17 SSV.dial CBV @22:40:42.21 CBV.dial SSV @22:40:42.96 Allowing 130s for 25.0ml of remaining 925.0ml @22:40:42.97 Intake.select :open @22:40:45.14 Intake @15.9psia, vacuum from 10.0 to 13.0psi @22:40:45.16 SS.reconfigure :inhaling 0.73ml/s @22:40:45.21 SS.seek 25ml @22:40:49.20 washCycleDA #1 of 2 @22:40:49.21 PSR.select :air @22:40:49.23 PSV.dial PSR @22:40:50.57 Pulling 0.5ml of PSR.wash @22:40:50.58 PSR.select :wash @22:40:50.70 PS.reconfigure :default @22:40:50.75 PS.seek 0.5ml @22:40:56.67 delay 5 seconds @22:41:01.69 PSR.select :air @22:41:01.81 PS.seek 2ml @22:41:17.95 PTV.dial :puck @22:41:18.70 PSV.dial PTV @22:41:19.57 PBV.dial :waste1 @22:41:20.01 PS.seek 1.5ml @22:41:21.14 Stroke completed -- waiting for pressure to equalize ... @22:41:21.15 SS.reconfigure :exhale @22:41:21.20 SS.hold @22:41:25.91 delay 3 seconds @22:41:28.93 PBV.dial between PSV and waste1 @22:41:29.31 PS.seek 0ml @22:41:45.45 delay 2:00 @22:41:49.90 Intake.select :close @22:41:50.06 SSV.dial :exhaust @22:41:50.84 Exhaust.select :open @22:41:50.96 SS.seek 0ml @22:41:59.94 Exhaust.select :close @22:42:00.05 SSV.dial CBV @22:42:00.07 CBV.dial SSV @22:42:00.85 Allowing 130s for 25.0ml of remaining 900.0ml @22:42:00.86 Intake.select :open @22:42:03.03 Intake @16.1psia, vacuum from 10.0 to 13.0psi @22:42:03.05 SS.reconfigure :inhaling 0.73ml/s @22:42:03.55 SS.seek 25ml @22:42:39.58 Stroke completed -- waiting for pressure to equalize ... @22:42:39.59 SS.reconfigure :exhale @22:42:39.64 SS.hold @22:43:06.10 Intake.select :close @22:43:06.72 SSV.dial :exhaust @22:43:07.51 Exhaust.select :open @22:43:07.63 SS.seek 0ml @22:43:16.67 Exhaust.select :close @22:43:16.78 SSV.dial CBV @22:43:16.81 CBV.dial SSV @22:43:17.58 Allowing 130s for 25.0ml of remaining 875.0ml @22:43:17.59 Intake.select :open @22:43:19.77 Intake @16.1psia, vacuum from 10.0 to 13.0psi @22:43:19.79 SS.reconfigure :inhaling 0.73ml/s @22:43:19.84 SS.seek 25ml @22:43:45.47 recoverPSRreagent08 @22:43:45.48 PTV.dial :puck @22:43:45.55 PBV.dial :waste1 @22:43:45.93 delay 30 seconds @22:43:56.00 Stroke completed -- waiting for pressure to equalize ... @22:43:56.01 SS.reconfigure :exhale @22:43:56.06 SS.hold @22:44:15.95 PBV.dial between PSV and waste1 @22:44:16.34 PSR.select :air @22:44:16.40 PSV.dial PSR @22:44:17.17 PS.reconfigure :fast @22:44:17.23 PS.seek 2.5ml @22:44:20.50 Intake.select :close @22:44:20.66 SSV.dial :exhaust @22:44:21.44 Exhaust.select :open @22:44:21.56 SS.seek 0ml @22:44:30.69 Exhaust.select :close @22:44:30.80 SSV.dial CBV @22:44:30.83 CBV.dial SSV @22:44:31.61 Allowing 130s for 25.0ml of remaining 850.0ml @22:44:31.62 Intake.select :open @22:44:33.80 Intake @16.0psia, vacuum from 10.0 to 13.0psi @22:44:33.82 SS.reconfigure :inhaling 0.73ml/s @22:44:33.87 SS.seek 25ml @22:44:41.29 PSV.dial PTV @22:44:42.18 delay 5 seconds @22:44:47.20 PSV.dial PSR @22:44:47.95 delay 5 seconds @22:44:52.97 PSV.dial PTV @22:44:53.73 delay 5 seconds @22:44:58.75 PSV.dial :air @22:44:59.65 PS.seek 0ml @22:45:09.34 Stroke completed -- waiting for pressure to equalize ... @22:45:09.35 SS.reconfigure :exhale @22:45:09.40 SS.hold @22:45:23.13 washCycleDA #2 of 2 @22:45:23.14 PSR.select :air @22:45:23.16 PSV.dial PSR @22:45:24.63 Pulling 0.5ml of PSR.wash @22:45:24.64 PSR.select :wash @22:45:24.76 PS.reconfigure :default @22:45:24.81 PS.seek 0.5ml @22:45:30.79 delay 5 seconds @22:45:33.94 Intake.select :close @22:45:34.10 SSV.dial :exhaust @22:45:34.89 Exhaust.select :open @22:45:35.01 SS.seek 0ml @22:45:35.81 PSR.select :air @22:45:35.93 PS.seek 2ml @22:45:44.06 Exhaust.select :close @22:45:44.17 SSV.dial CBV @22:45:44.20 CBV.dial SSV @22:45:44.98 Allowing 130s for 25.0ml of remaining 825.0ml @22:45:45.00 Intake.select :open @22:45:47.17 Intake @16.1psia, vacuum from 10.0 to 13.0psi @22:45:47.58 SS.reconfigure :inhaling 0.73ml/s @22:45:47.69 SS.seek 25ml @22:45:52.10 PTV.dial :puck @22:45:52.18 PSV.dial PTV @22:45:52.93 PBV.dial :waste1 @22:45:53.36 PS.seek 1.5ml @22:45:59.26 delay 3 seconds @22:46:02.28 PBV.dial between PSV and waste1 @22:46:02.65 PS.seek 0ml @22:46:18.79 delay 2:00 @22:46:20.52 Vacuum too high -- waiting up to 40 seconds @22:46:20.54 SS.reconfigure :exhale @22:46:20.59 SS.hold @22:46:28.20 Intake @17.4psia, vacuum from 10.0 to 13.0psi @22:46:28.22 SS.reconfigure :inhaling 0.59ml/s @22:46:28.27 SS.seek 25ml @22:46:32.57 Stroke completed -- waiting for pressure to equalize ... @22:46:32.59 SS.reconfigure :exhale @22:46:32.63 SS.hold @22:46:49.19 Intake.select :close @22:46:49.35 SSV.dial :exhaust @22:46:50.14 Exhaust.select :open @22:46:50.26 SS.seek 0ml @22:46:59.29 Exhaust.select :close @22:46:59.40 SSV.dial CBV @22:46:59.42 CBV.dial SSV @22:47:00.22 Allowing 130s for 25.0ml of remaining 800.0ml @22:47:00.23 Intake.select :open @22:47:02.40 Intake @16.1psia, vacuum from 10.0 to 13.0psi @22:47:02.42 SS.reconfigure :inhaling 0.59ml/s @22:47:02.47 SS.seek 25ml @22:47:46.98 Stroke completed -- waiting for pressure to equalize ... @22:47:46.99 SS.reconfigure :exhale @22:47:47.04 SS.hold @22:48:07.94 Intake.select :close @22:48:08.11 SSV.dial :exhaust @22:48:08.88 Exhaust.select :open @22:48:09.00 SS.seek 0ml @22:48:17.81 Exhaust.select :close @22:48:17.92 SSV.dial CBV @22:48:17.94 CBV.dial SSV @22:48:18.73 Allowing 130s for 25.0ml of remaining 775.0ml @22:48:18.75 Intake.select :open @22:48:18.81 recoverPSRreagent08 @22:48:18.82 PTV.dial :puck @22:48:18.89 PBV.dial :waste1 @22:48:19.27 delay 30 seconds @22:48:20.93 Intake @16.1psia, vacuum from 10.0 to 13.0psi @22:48:21.40 SS.reconfigure :inhaling 0.59ml/s @22:48:21.45 SS.seek 25ml @22:48:49.29 PBV.dial between PSV and waste1 @22:48:49.66 PSR.select :air @22:48:49.68 PSV.dial PSR @22:48:50.45 PS.reconfigure :fast @22:48:50.50 PS.seek 2.5ml @22:49:06.02 Stroke completed -- waiting for pressure to equalize ... @22:49:06.04 SS.reconfigure :exhale @22:49:06.09 SS.hold @22:49:14.70 PSV.dial PTV @22:49:15.47 delay 5 seconds @22:49:20.51 PSV.dial PSR @22:49:21.27 delay 5 seconds @22:49:26.29 PSV.dial PTV @22:49:27.17 delay 5 seconds @22:49:28.57 Intake.select :close @22:49:28.73 SSV.dial :exhaust @22:49:29.51 Exhaust.select :open @22:49:29.63 SS.seek 0ml @22:49:32.19 PSV.dial :air @22:49:32.98 PS.seek 0ml @22:49:38.41 Exhaust.select :close @22:49:38.53 SSV.dial CBV @22:49:38.56 CBV.dial SSV @22:49:39.34 Allowing 130s for 25.0ml of remaining 750.0ml @22:49:39.35 Intake.select :open @22:49:41.53 Intake @16.0psia, vacuum from 10.0 to 13.0psi @22:49:41.55 SS.reconfigure :inhaling 0.59ml/s @22:49:41.60 SS.seek 25ml @22:49:56.60 getPMLReagent08 @22:49:56.61 PRV.dial :air @22:49:57.08 delay 5 seconds @22:50:02.10 PTV.dial :PRVmixing @22:50:03.44 delay 5 seconds @22:50:08.46 PRV.dial :ab2 @22:50:08.92 PTV.dial :PRVmixing @22:50:09.11 PSV.dial :mixloop @22:50:09.71 PS.reconfigure :default @22:50:09.76 PS.seek 0.3ml @22:50:13.69 delay 2 seconds @22:50:15.71 PRV.dial :air @22:50:16.23 PS.seek 0.55ml @22:50:19.66 addPMLReagent08 @22:50:19.67 PSV.dial :air @22:50:20.26 PS.seek 2ml @22:50:26.35 Stroke completed -- waiting for pressure to equalize ... @22:50:26.36 SS.reconfigure :exhale @22:50:26.41 SS.hold @22:50:35.84 PSV.dial :PML @22:50:36.29 PTV.dial :mixing @22:50:37.07 PBV.dial :waste1 @22:50:37.57 PS.seek 1.85ml @22:50:39.90 PBV.dial between PSV and waste1 @22:50:40.27 PS.seek 0ml @22:50:48.81 Intake.select :close @22:50:48.97 SSV.dial :exhaust @22:50:49.74 Exhaust.select :open @22:50:49.86 SS.seek 0ml @22:50:58.85 Exhaust.select :close @22:50:58.96 SSV.dial CBV @22:50:58.99 CBV.dial SSV @22:50:59.77 Allowing 130s for 25.0ml of remaining 725.0ml @22:50:59.79 Intake.select :open @22:51:00.00 delay 2 seconds @22:51:01.95 Intake @16.1psia, vacuum from 10.0 to 13.0psi @22:51:01.97 SS.reconfigure :inhaling 0.59ml/s @22:51:02.02 SS.seek 25ml @22:51:02.07 delay 8:00 @22:51:46.44 Stroke completed -- waiting for pressure to equalize ... @22:51:46.45 SS.reconfigure :exhale @22:51:46.97 SS.hold @22:52:09.50 Intake.select :close @22:52:09.67 SSV.dial :exhaust @22:52:10.44 Exhaust.select :open @22:52:10.56 SS.seek 0ml @22:52:19.60 Exhaust.select :close @22:52:20.18 SSV.dial CBV @22:52:20.21 CBV.dial SSV @22:52:20.98 Allowing 130s for 25.0ml of remaining 700.0ml @22:52:20.99 Intake.select :open @22:52:23.16 Intake @16.0psia, vacuum from 10.0 to 13.0psi @22:52:23.18 SS.reconfigure :inhaling 0.59ml/s @22:52:23.23 SS.seek 25ml @22:53:07.25 Stroke completed -- waiting for pressure to equalize ... @22:53:07.27 SS.reconfigure :exhale @22:53:07.31 SS.hold @22:53:32.30 Intake.select :close @22:53:32.47 SSV.dial :exhaust @22:53:33.25 Exhaust.select :open @22:53:33.37 SS.seek 0ml @22:53:42.21 Exhaust.select :close @22:53:42.32 SSV.dial CBV @22:53:42.34 CBV.dial SSV @22:53:43.15 Allowing 130s for 25.0ml of remaining 675.0ml @22:53:43.16 Intake.select :open @22:53:45.33 Intake @16.1psia, vacuum from 10.0 to 13.0psi @22:53:45.35 SS.reconfigure :inhaling 0.59ml/s @22:53:45.40 SS.seek 25ml @22:54:26.04 Vacuum too high -- waiting up to 40 seconds @22:54:26.06 SS.reconfigure :exhale @22:54:26.11 SS.hold @22:54:37.85 Intake @17.0psia, vacuum from 10.0 to 13.0psi @22:54:37.87 SS.reconfigure :inhaling 0.47ml/s @22:54:37.92 SS.seek 25ml @22:54:43.03 Stroke completed -- waiting for pressure to equalize ... @22:54:43.04 SS.reconfigure :exhale @22:54:43.09 SS.hold @22:54:57.52 Intake.select :close @22:54:57.68 SSV.dial :exhaust @22:54:58.44 Exhaust.select :open @22:54:58.56 SS.seek 0ml @22:55:07.27 Exhaust.select :close @22:55:07.38 SSV.dial CBV @22:55:07.41 CBV.dial SSV @22:55:08.17 Allowing 130s for 25.0ml of remaining 650.0ml @22:55:08.19 Intake.select :open @22:55:10.82 Intake @16.0psia, vacuum from 10.0 to 13.0psi @22:55:10.84 SS.reconfigure :inhaling 0.47ml/s @22:55:10.89 SS.seek 25ml @22:56:06.64 Stroke completed -- waiting for pressure to equalize ... @22:56:06.65 SS.reconfigure :exhale @22:56:06.70 SS.hold @22:56:31.62 Intake.select :close @22:56:31.85 SSV.dial :exhaust @22:56:32.62 Exhaust.select :open @22:56:32.74 SS.seek 0ml @22:56:42.00 Exhaust.select :close @22:56:42.12 SSV.dial CBV @22:56:42.14 CBV.dial SSV @22:56:42.92 Allowing 130s for 25.0ml of remaining 625.0ml @22:56:42.94 Intake.select :open @22:56:45.10 Intake @15.9psia, vacuum from 10.0 to 13.0psi @22:56:45.12 SS.reconfigure :inhaling 0.47ml/s @22:56:45.17 SS.seek 25ml @22:57:39.59 Stroke completed -- waiting for pressure to equalize ... @22:57:39.60 SS.reconfigure :exhale @22:57:39.65 SS.hold @22:58:06.28 Intake.select :close @22:58:06.44 SSV.dial :exhaust @22:58:07.23 Exhaust.select :open @22:58:07.35 SS.seek 0ml @22:58:16.39 Exhaust.select :close @22:58:16.50 SSV.dial CBV @22:58:16.54 CBV.dial SSV @22:58:17.31 Allowing 130s for 25.0ml of remaining 600.0ml @22:58:17.33 Intake.select :open @22:58:19.49 Intake @16.1psia, vacuum from 10.0 to 13.0psi @22:58:19.51 SS.reconfigure :inhaling 0.47ml/s @22:58:19.56 SS.seek 25ml @22:59:02.11 recoverPRVreagent08 @22:59:02.12 PTV.dial :mixing @22:59:02.19 PBV.dial :waste1 @22:59:02.57 delay 30 seconds @22:59:14.76 Stroke completed -- waiting for pressure to equalize ... @22:59:14.77 SS.reconfigure :exhale @22:59:14.82 SS.hold @22:59:32.59 PBV.dial between PSV and waste1 @22:59:32.96 PSV.dial :air @22:59:33.56 PS.reconfigure :fast @22:59:33.61 PS.seek 2.5ml @22:59:45.43 Intake.select :close @22:59:45.59 SSV.dial :exhaust @22:59:46.37 Exhaust.select :open @22:59:46.49 SS.seek 0ml @22:59:55.34 Exhaust.select :close @22:59:55.45 SSV.dial CBV @22:59:55.49 CBV.dial SSV @22:59:56.26 Allowing 130s for 25.0ml of remaining 575.0ml @22:59:56.28 Intake.select :open @22:59:57.86 PSV.dial :mixloop @22:59:58.32 delay 2 seconds @22:59:58.45 Intake @16.1psia, vacuum from 10.0 to 13.0psi @22:59:58.47 SS.reconfigure :inhaling 0.47ml/s @22:59:58.52 SS.seek 25ml @23:00:00.33 PSV.dial :air @23:00:00.94 delay 2 seconds @23:00:02.96 PSV.dial :mixloop @23:00:03.88 delay 2 seconds @23:00:05.90 PSV.dial :air @23:00:06.37 PS.seek 0ml @23:00:29.80 washCycleDA #1 of 3 @23:00:29.81 PSR.select :air @23:00:29.83 PSV.dial PSR @23:00:31.18 Pulling 0.5ml of PSR.wash @23:00:31.19 PSR.select :wash @23:00:31.31 PS.reconfigure :default @23:00:31.36 PS.seek 0.5ml @23:00:37.27 delay 5 seconds @23:00:42.29 PSR.select :air @23:00:42.42 PS.seek 2ml @23:00:48.60 Vacuum too high -- waiting up to 40 seconds @23:00:48.61 SS.reconfigure :exhale @23:00:48.67 SS.hold @23:00:58.71 PTV.dial :puck @23:00:59.46 PSV.dial PTV @23:01:00.20 PBV.dial :waste1 @23:01:00.64 PS.seek 1.5ml @23:01:05.61 Intake @18.0psia, vacuum from 10.0 to 13.0psi @23:01:05.63 SS.reconfigure :inhaling 0.38ml/s @23:01:05.68 SS.seek 25ml @23:01:06.67 delay 3 seconds @23:01:09.69 PBV.dial between PSV and waste1 @23:01:10.07 PS.seek 0ml @23:01:12.57 Stroke completed -- waiting for pressure to equalize ... @23:01:12.58 SS.reconfigure :exhale @23:01:12.63 SS.hold @23:01:26.17 delay 2:00 @23:01:28.96 Intake.select :close @23:01:29.53 SSV.dial :exhaust @23:01:30.37 Exhaust.select :open @23:01:30.49 SS.seek 0ml @23:01:39.32 Exhaust.select :close @23:01:39.43 SSV.dial CBV @23:01:39.46 CBV.dial SSV @23:01:40.24 Allowing 130s for 25.0ml of remaining 550.0ml @23:01:40.26 Intake.select :open @23:01:42.44 Intake @16.1psia, vacuum from 10.0 to 13.0psi @23:01:42.46 SS.reconfigure :inhaling 0.38ml/s @23:01:42.51 SS.seek 25ml @23:02:51.08 Stroke completed -- waiting for pressure to equalize ... @23:02:51.10 SS.reconfigure :exhale @23:02:51.15 SS.hold @23:03:21.91 Intake.select :close @23:03:22.07 SSV.dial :exhaust @23:03:22.83 Exhaust.select :open @23:03:22.95 SS.seek 0ml @23:03:26.19 recoverPSRreagent08 @23:03:26.20 PTV.dial :puck @23:03:26.22 PBV.dial :waste1 @23:03:26.60 delay 30 seconds @23:03:32.01 Exhaust.select :close @23:03:32.12 SSV.dial CBV @23:03:32.15 CBV.dial SSV @23:03:32.91 Allowing 130s for 25.0ml of remaining 525.0ml @23:03:32.93 Intake.select :open @23:03:35.10 Intake @16.1psia, vacuum from 10.0 to 13.0psi @23:03:35.12 SS.reconfigure :inhaling 0.38ml/s @23:03:35.17 SS.seek 25ml @23:03:56.62 PBV.dial between PSV and waste1 @23:03:56.98 PSR.select :air @23:03:57.00 PSV.dial PSR @23:03:57.77 PS.reconfigure :fast @23:03:57.82 PS.seek 2.5ml @23:04:21.76 PSV.dial PTV @23:04:22.53 delay 5 seconds @23:04:27.55 PSV.dial PSR @23:04:28.30 delay 5 seconds @23:04:33.32 PSV.dial PTV @23:04:34.09 delay 5 seconds @23:04:39.11 PSV.dial :air @23:04:39.88 PS.seek 0ml @23:04:43.19 Stroke completed -- waiting for pressure to equalize ... @23:04:43.20 SS.reconfigure :exhale @23:04:43.25 SS.hold @23:05:03.36 washCycleDA #2 of 3 @23:05:03.37 PSR.select :air @23:05:03.39 PSV.dial PSR @23:05:04.73 Pulling 0.5ml of PSR.wash @23:05:04.74 PSR.select :wash @23:05:04.86 PS.reconfigure :default @23:05:04.91 PS.seek 0.5ml @23:05:10.82 delay 5 seconds @23:05:15.84 PSR.select :air @23:05:16.05 PS.seek 2ml @23:05:16.12 Intake.select :close @23:05:16.77 SSV.dial :exhaust @23:05:17.55 Exhaust.select :open @23:05:17.67 SS.seek 0ml @23:05:26.71 Exhaust.select :close @23:05:26.82 SSV.dial CBV @23:05:26.85 CBV.dial SSV @23:05:27.63 Allowing 130s for 25.0ml of remaining 500.0ml @23:05:27.65 Intake.select :open @23:05:29.81 Intake @16.1psia, vacuum from 10.0 to 13.0psi @23:05:29.83 SS.reconfigure :inhaling 0.38ml/s @23:05:29.88 SS.seek 25ml @23:05:32.26 PTV.dial :puck @23:05:32.28 PSV.dial PTV @23:05:33.03 PBV.dial :waste1 @23:05:33.45 PS.seek 1.5ml @23:05:39.39 delay 3 seconds @23:05:42.41 PBV.dial between PSV and waste1 @23:05:42.78 PS.seek 0ml @23:05:58.95 delay 2:00 @23:06:36.68 Vacuum too high -- waiting up to 40 seconds @23:06:36.70 SS.reconfigure :exhale @23:06:36.76 SS.hold @23:06:57.93 Intake @17.6psia, vacuum from 10.0 to 13.0psi @23:06:57.95 SS.reconfigure :inhaling 0.31ml/s @23:06:58.01 SS.seek 25ml @23:07:00.02 Stroke completed -- waiting for pressure to equalize ... @23:07:00.03 SS.reconfigure :exhale @23:07:00.08 SS.hold @23:07:18.94 Intake.select :close @23:07:19.10 SSV.dial :exhaust @23:07:19.86 Exhaust.select :open @23:07:19.99 SS.seek 0ml @23:07:29.19 Exhaust.select :close @23:07:29.30 SSV.dial CBV @23:07:29.35 CBV.dial SSV @23:07:30.10 Allowing 130s for 25.0ml of remaining 475.0ml @23:07:30.11 Intake.select :open @23:07:32.28 Intake @16.2psia, vacuum from 10.0 to 13.0psi @23:07:32.30 SS.reconfigure :inhaling 0.31ml/s @23:07:32.35 SS.seek 25ml @23:07:58.98 recoverPSRreagent08 @23:07:58.99 PTV.dial :puck @23:07:59.00 PBV.dial :waste1 @23:07:59.37 delay 30 seconds @23:08:29.40 PBV.dial between PSV and waste1 @23:08:29.77 PSR.select :air @23:08:29.79 PSV.dial PSR @23:08:30.56 PS.reconfigure :fast @23:08:30.61 PS.seek 2.5ml @23:08:54.60 PSV.dial PTV @23:08:55.50 delay 5 seconds @23:08:59.39 Stroke completed -- waiting for pressure to equalize ... @23:08:59.40 SS.reconfigure :exhale @23:08:59.45 SS.hold @23:09:00.51 PSV.dial PSR @23:09:01.26 delay 5 seconds @23:09:06.28 PSV.dial PTV @23:09:07.04 delay 5 seconds @23:09:12.13 PSV.dial :air @23:09:12.90 PS.seek 0ml @23:09:34.23 Intake.select :close @23:09:34.39 SSV.dial :exhaust @23:09:35.15 Exhaust.select :open @23:09:35.27 SS.seek 0ml @23:09:36.39 washCycleDA #3 of 3 @23:09:36.40 PSR.select :air @23:09:36.41 PSV.dial PSR @23:09:37.76 Pulling 0.5ml of PSR.wash @23:09:37.77 PSR.select :wash @23:09:37.89 PS.reconfigure :default @23:09:37.93 PS.seek 0.5ml @23:09:43.95 delay 5 seconds @23:09:44.04 Exhaust.select :close @23:09:44.59 SSV.dial CBV @23:09:44.61 CBV.dial SSV @23:09:45.41 Allowing 130s for 25.0ml of remaining 450.0ml @23:09:45.42 Intake.select :open @23:09:47.59 Intake @16.1psia, vacuum from 10.0 to 13.0psi @23:09:47.61 SS.reconfigure :inhaling 0.31ml/s @23:09:47.66 SS.seek 25ml @23:09:48.96 PSR.select :air @23:09:49.09 PS.seek 2ml @23:10:05.29 PTV.dial :puck @23:10:05.30 PSV.dial PTV @23:10:06.05 PBV.dial :waste1 @23:10:06.49 PS.seek 1.5ml @23:10:12.45 delay 3 seconds @23:10:15.46 PBV.dial between PSV and waste1 @23:10:15.84 PS.seek 0ml @23:10:32.20 delay 2:00 @23:11:16.59 Stroke completed -- waiting for pressure to equalize ... @23:11:16.60 SS.reconfigure :exhale @23:11:16.65 SS.hold @23:11:53.97 Intake.select :close @23:11:54.13 SSV.dial :exhaust @23:11:54.90 Exhaust.select :open @23:11:55.02 SS.seek 0ml @23:12:03.92 Exhaust.select :close @23:12:04.03 SSV.dial CBV @23:12:04.05 CBV.dial SSV @23:12:04.83 Allowing 130s for 25.0ml of remaining 425.0ml @23:12:04.84 Intake.select :open @23:12:07.01 Intake @16.3psia, vacuum from 10.0 to 13.0psi @23:12:07.03 SS.reconfigure :inhaling 0.31ml/s @23:12:07.08 SS.seek 25ml @23:12:32.22 recoverPSRreagent08 @23:12:32.23 PTV.dial :puck @23:12:32.24 PBV.dial :waste1 @23:12:32.62 delay 30 seconds @23:13:02.64 PBV.dial between PSV and waste1 @23:13:03.02 PSR.select :air @23:13:03.04 PSV.dial PSR @23:13:03.81 PS.reconfigure :fast @23:13:03.86 PS.seek 2.5ml @23:13:27.72 PSV.dial PTV @23:13:28.49 delay 5 seconds @23:13:33.51 PSV.dial PSR @23:13:34.27 delay 5 seconds @23:13:35.52 Stroke completed -- waiting for pressure to equalize ... @23:13:35.54 SS.reconfigure :exhale @23:13:35.59 SS.hold @23:13:39.29 PSV.dial PTV @23:13:40.09 delay 5 seconds @23:13:45.12 PSV.dial :air @23:13:45.88 PS.seek 0ml @23:14:09.22 Gate.power :camera,:ON @23:14:09.24 Mixing DA Substrates @23:14:09.25 PSR.select :air @23:14:09.26 PSV.dial PSR @23:14:10.68 Pulling 0.25ml of PSR.sub1 @23:14:10.70 PSR.select :sub1 @23:14:11.19 PS.reconfigure :default @23:14:11.36 PS.seek 0.25ml @23:14:14.81 delay 5 seconds @23:14:14.85 SS.reconfigure :default @23:14:14.90 SS.hold @23:14:14.97 Intake.select :close @23:14:15.13 ShallowSampler::Clogged in WCR -- Filtering too Slowly after 600.0ml @23:14:15.15 SSV.dial :exhaust @23:14:15.95 Exhaust.select :open @23:14:16.07 SS.reconfigure :exhale @23:14:16.12 SS.seek 0ml @23:14:19.83 Pulling 0.25ml of PSR.sub2 @23:14:19.85 PSR.select :sub2 @23:14:19.96 PS.seek 0.5ml @23:14:23.28 delay 5 seconds @23:14:25.05 Exhaust.select :close @23:14:25.17 Sampled 600.0ml @23:14:25.19 Drying puck before fixing for 5:00 @23:14:25.20 CBV.dial :waste2b @23:14:25.98 delay 1:00 @23:14:28.30 PSR.select :air @23:14:28.42 PS.seek 2ml @23:14:44.56 PTV.dial :puck @23:14:44.58 PSV.dial PTV @23:14:45.35 PBV.dial :waste1 @23:14:45.80 PS.seek 1.5ml @23:14:51.75 delay 3 seconds @23:14:54.77 PBV.dial between PSV and waste1 @23:14:55.15 PS.seek 0ml @23:15:11.35 delay 10 seconds @23:15:21.38 recoverPSRreagent08 PTV.dial :puck @23:15:21.39 PBV.dial :waste1 @23:15:21.77 delay 30 seconds @23:15:26.00 CSV.dial :air @23:15:26.49 CS.reconfigure :fast @23:15:26.55 CS.seek 10ml @23:15:51.36 Pumping wcr puck from tube 4 up to 35psi... @23:15:51.37 CBV.dial SSV @23:15:51.79 PBV.dial between PSV and waste1 @23:15:52.18 PSR.select :air @23:15:52.21 PSV.dial PSR @23:15:52.70 CBV.dial between CSV and waste2b @23:15:52.99 PS.reconfigure :fast @23:15:53.04 PS.seek 2.5ml @23:15:54.16 CSV.dial between air and CTV @23:15:54.52 CS.seek 7.105ml @23:16:02.17 CTV.dial CSV,avoiding: :air @23:16:02.20 CSV.dial CTV @23:16:03.05 CS.reconfigure :pressurize @23:16:03.10 CS.seek 0ml @23:16:17.28 PSV.dial PTV @23:16:18.07 delay 5 seconds @23:16:21.09 CBV.dial :waste2b @23:16:21.43 CS.reconfigure :default @23:16:21.49 CS.seek 0ml @23:16:22.86 delay 1:00 @23:16:23.08 PSV.dial PSR @23:16:23.85 delay 5 seconds @23:16:28.87 PSV.dial PTV @23:16:29.64 delay 5 seconds @23:16:34.66 PSV.dial :air @23:16:35.46 PS.seek 0ml @23:16:59.38 Additional air clear @23:16:59.39 PSR.select :air @23:16:59.40 PSV.dial PSR @23:17:00.77 Pulling 2ml of PSR.air @23:17:00.78 PSR.select :air @23:17:00.80 PS.reconfigure :default @23:17:00.85 PS.seek 2ml @23:17:22.09 delay 5 seconds @23:17:22.88 CTV.dial :air,avoiding: :intake @23:17:24.23 Fix #1 of 2 (with RNAlater) for 2:30 @23:17:24.25 CSV.dial CSR @23:17:24.68 Pulling 2ml of CSR.RNAlater @23:17:24.69 CSR.select :RNAlater @23:17:24.81 CS.seek 2ml @23:17:27.11 PTV.dial :puck @23:17:27.13 PSV.dial PTV @23:17:27.89 PBV.dial :waste1 @23:17:28.34 PS.seek 1.5ml @23:17:31.01 delay 5 seconds @23:17:34.28 delay 3 seconds @23:17:36.03 CSR.select :air @23:17:36.14 CS.reconfigure :fast @23:17:36.20 CS.seek 10ml @23:17:37.30 PBV.dial between PSV and waste1 @23:17:37.67 delay 1 second @23:17:38.69 PSV.dial :waste1 @23:17:39.78 PS.seek 0ml @23:17:55.99 Processing Puck Heater at 28C before imaging @23:17:56.00 PH.coast @23:17:56.12 Imaging da puck in Processing Clamp while storing it to tube 3 @23:17:56.28 Pumping wcr puck from tube 4 up to 35psi... @23:17:56.33 CBV.dial SSV @23:17:56.86 SC.reconfigure :tight @23:17:56.94 SC.seek 3 @23:17:57.59 CBV.dial between CSV and waste2b @23:17:58.58 CSV.dial between air and CTV @23:17:59.20 CS.seek 7.026ml @23:18:00.56 SC.configure :limp @23:18:00.78 Elbow.reconfigure :tight @23:18:00.83 Elbow.seek 3 @23:18:02.62 Forearm.reconfigure :tight @23:18:02.67 Forearm.seek 3 + 450 counts @23:18:03.69 SE.reconfigure :pick @23:18:03.75 SE.seek :beamBreak @23:18:05.07 SE.configure :default @23:18:05.23 Elbow.reconfigure :limp @23:18:05.28 Forearm.reconfigure :loose @23:18:05.33 Forearm.seek 3 @23:18:05.61 Elbow.hold @23:18:05.68 Elbow.reconfigure :tight @23:18:05.78 SE.seek 27.11 Pucks below Top Plate @23:18:06.28 Forearm.force -20 @23:18:06.33 Forearm.seek 3 + 309 counts @23:18:06.62 Forearm.reconfigure :tight @23:18:06.67 Forearm.seek 3 + 450 counts @23:18:06.87 SE.seek 28.11 Pucks below Top Plate @23:18:06.98 CTV.dial CSV,avoiding: :air @23:18:07.01 CSV.dial CTV @23:18:07.52 Forearm.reconfigure :loose @23:18:07.57 Forearm.seek :retract @23:18:08.41 CS.reconfigure :pressurize @23:18:08.46 CS.seek 0ml @23:18:08.52 Elbow.seek PC @23:18:11.57 Elbow.reconfigure :loose @23:18:11.68 Forearm.seek PC @23:18:12.70 Hand.close @23:18:13.33 PC.seek :open @23:18:18.11 Forearm.seek :retract @23:18:19.05 Elbow.reconfigure :tight @23:18:19.10 Elbow.seek 3 @23:18:21.73 Forearm.reconfigure :tight @23:18:21.78 Forearm.seek 3 + 450 counts @23:18:22.75 Hand.open @23:18:23.40 SE.seek 27.76 Pucks below Top Plate @23:18:23.69 SE.reconfigure :seal @23:18:23.74 SE.seek 26.79 Pucks below Top Plate @23:18:24.25 Lights, camera, ACTION! @23:18:24.29 PuckCamera: snap -bin=2,2 -auto /var/log/esp/da23nov3023h900ml.tif @23:18:26.90 delay 2:30 @23:18:43.80 Waiting >20 seconds for da to relinquish FlushPuck @23:18:44.67 SX HX9/16 (USB:11): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 17.081 seconds /var/log/esp/da23nov3023h900ml.tif: TIFF Upload Complete @23:18:44.70 PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da23nov3023h900ml15s.tif @23:19:00.31 SX HX9/16 (USB:11): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15 seconds /var/log/esp/midres/da23nov3023h900ml15s.tif: TIFF Upload Complete @23:19:00.33 PuckCamera: snap -bin=2,2 20.0 /var/log/esp/midres/da23nov3023h900ml20s.tif @23:19:20.95 SX HX9/16 (USB:11): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 20 seconds /var/log/esp/midres/da23nov3023h900ml20s.tif: TIFF Upload Complete @23:19:20.97 Gate.power :camera,:OFF @23:19:20.99 DAfans.email "17.081 sec auto-exposure",Subject: "da23nov3023h900ml images!" @23:19:21.41 Elbow.seek 3 @23:19:21.64 Forearm.seek 3 + 450 counts @23:19:21.74 SE.reconfigure :gently @23:19:21.79 SE.seek 27.51 Pucks below Top Plate @23:19:22.50 Elbow.reconfigure :limp @23:19:22.56 Forearm.reconfigure :loose @23:19:22.63 Forearm.seek 3 @23:19:22.91 Elbow.hold @23:19:23.00 Elbow.reconfigure :tight @23:19:23.06 SE.reconfigure :default @23:19:23.11 SE.seek 27.69 Pucks below Top Plate @23:19:24.15 Forearm.force -20 @23:19:24.20 Forearm.seek 3 + 310 counts @23:19:24.52 Forearm.reconfigure :tight @23:19:24.57 Forearm.seek 3 + 450 counts @23:19:25.14 SE.seek :down @23:19:26.05 SE.stop @23:19:26.44 Forearm.reconfigure :loose @23:19:26.55 Forearm.seek :retract @23:19:27.72 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @23:19:28.13 Elbow.seek FlushPuck::Garage @23:19:30.19 Elbow.reconfigure :loose @23:19:30.41 Forearm.seek :garage @23:19:31.40 Hand.close @23:19:32.46 Forearm.seek :retract @23:19:33.49 Elbow.reconfigure :tight @23:19:33.59 Elbow.seek PC @23:19:34.97 Elbow.reconfigure :loose @23:19:35.20 Forearm.reconfigure :tight @23:19:35.30 Forearm.seek PC @23:19:36.95 PC.seek :closed @23:19:41.76 Processing Clamp onSpring/offSpring for 16/26 samples, on/off torque 1.62 @23:19:41.98 Hand.open @23:19:42.78 Forearm.reconfigure :loose @23:19:42.88 Forearm.seek :retract @23:19:44.89 PSR.select :air @23:19:44.93 PSV.dial PSR @23:19:45.44 Pulling 0.5ml of PSR.flush @23:19:45.50 PSR.select :flush @23:19:45.74 PS.seek 0.5ml @23:19:51.68 delay 5 seconds @23:19:55.93 upsync @23:19:56.71 PSR.select :air @23:19:57.84 PS.seek 2.5ml @23:20:19.12 delay 1 second @23:20:20.14 PRV.dial :waste2,avoiding: :ab2 @23:20:22.01 PTV.dial :PRVmixing @23:20:23.34 PSV.dial :mixloop @23:20:24.40 PS.seek :empty @23:20:50.90 PSR.select :air @23:20:50.92 PSV.dial PSR @23:20:51.98 Pulling 0.5ml of PSR.flush @23:20:51.99 PSR.select :flush @23:20:52.11 PS.seek 0.5ml @23:20:56.93 CBV.dial :waste2b @23:20:57.30 CS.reconfigure :default @23:20:57.36 CS.seek 0ml @23:20:58.06 delay 5 seconds @23:20:58.94 delay 1:00 @23:21:03.08 PSR.select :air @23:21:03.20 PS.seek 2.5ml @23:21:24.49 delay 1 second @23:21:25.51 PBV.dial :waste1 @23:21:25.87 PTV.dial :mixing @23:21:26.64 PSV.dial :mixloop @23:21:27.83 PS.seek :empty @23:21:54.21 PSV.dial PSR @23:21:55.26 PSR.select :flush @23:21:55.39 PS.seek 1ml @23:21:58.96 CTV.dial :air,avoiding: :intake @23:22:00.29 Fix #2 of 2 (with RNAlater) for 2:30 @23:22:00.30 CSV.dial CSR @23:22:00.74 Pulling 2ml of CSR.RNAlater @23:22:00.75 CSR.select :RNAlater @23:22:00.87 CS.seek 2ml @23:22:06.49 PSR.select :air @23:22:06.61 PS.seek 2.5ml @23:22:07.03 delay 5 seconds @23:22:12.05 CSR.select :air @23:22:12.17 CS.reconfigure :fast @23:22:12.22 CS.seek 10ml @23:22:22.71 PSV.dial PTV @23:22:23.48 PTV.dial :puck @23:22:24.21 PBV.dial :waste1 @23:22:24.23 PS.seek 0ml @23:22:31.97 Pumping wcr puck from tube 4 up to 35psi... @23:22:31.99 CBV.dial SSV @23:22:33.25 CBV.dial between CSV and waste2b @23:22:34.24 CSV.dial between air and CTV @23:22:34.85 CS.seek 6.98ml @23:22:42.54 CTV.dial CSV,avoiding: :air @23:22:42.56 CSV.dial CTV @23:22:43.96 CS.reconfigure :pressurize @23:22:44.01 CS.seek 0ml @23:22:50.62 PSV.dial PSR @23:22:51.37 PSR.select :flush @23:22:51.49 PS.seek 0.5ml @23:22:57.46 PSR.select :air @23:22:57.58 PS.seek 2.5ml @23:22:58.87 delay 2:30 @23:23:18.80 PSV.dial PTV @23:23:19.57 PBV.dial PSV @23:23:20.14 PS.seek 0ml @23:23:46.46 PSV.dial PBV @23:23:46.94 PS.seek 2.5ml @23:24:13.37 PSV.dial :waste1 @23:24:14.23 PS.seek 0ml @23:24:40.55 PSV.dial PSR @23:24:41.01 PSR.select :air @23:24:41.03 PS.seek 2.5ml @23:25:07.40 PSV.dial PTV @23:25:08.26 PBV.dial PSV @23:25:08.45 PS.seek 0ml @23:25:28.89 CBV.dial :waste2b @23:25:29.24 CS.reconfigure :default @23:25:29.29 CS.seek 0ml @23:25:33.47 delay 1:00 @23:25:34.83 PSV.dial PBV @23:25:35.29 PS.seek 2.5ml @23:26:01.68 PSV.dial :waste1 @23:26:02.42 PS.seek 0ml @23:26:28.74 PSV.dial PSR @23:26:29.21 PSR.select :flush @23:26:29.33 PS.seek 1ml @23:26:33.49 CTV.dial :air,avoiding: :intake @23:26:34.87 Storing wcr puck in Collection Clamp to tube 3 @23:26:35.76 Elbow.reconfigure :tight @23:26:35.81 Elbow.seek 3 @23:26:38.42 Forearm.reconfigure :tight @23:26:38.47 Forearm.seek 3 + 450 counts @23:26:39.52 SE.reconfigure :pick @23:26:39.58 SE.seek :beamBreak @23:26:40.30 PSR.select :air @23:26:40.42 PS.seek 2.5ml @23:26:40.81 SE.configure :default @23:26:40.97 Elbow.reconfigure :limp @23:26:41.03 Forearm.reconfigure :loose @23:26:41.08 Forearm.seek 3 @23:26:41.37 Elbow.hold @23:26:41.44 Elbow.reconfigure :tight @23:26:41.55 SE.seek 28.1 Pucks below Top Plate @23:26:42.00 Forearm.force -20 @23:26:42.05 Forearm.seek 3 + 281 counts @23:26:42.34 Forearm.reconfigure :tight @23:26:42.39 Forearm.seek 3 + 450 counts @23:26:42.58 SE.seek 29.1 Pucks below Top Plate @23:26:43.21 Forearm.reconfigure :loose @23:26:43.26 Forearm.seek :retract @23:26:44.20 Elbow.seek CC @23:26:45.82 Elbow.reconfigure :loose @23:26:46.38 Forearm.seek CC @23:26:47.37 Hand.close @23:26:47.95 CC.seek :open @23:26:52.71 Forearm.seek :retract @23:26:53.75 Elbow.reconfigure :tight @23:26:53.80 Elbow.seek 3 @23:26:55.58 Elbow.reconfigure :limp @23:26:55.64 Forearm.seek 3 @23:26:56.55 PSV.dial PTV @23:26:56.70 Elbow.hold @23:26:56.77 Elbow.reconfigure :tight @23:26:56.82 Hand.open @23:26:57.41 PTV.dial :puck @23:26:57.58 PBV.dial :waste1 @23:26:57.70 Forearm.force -20 @23:26:57.75 Forearm.seek 3 + 314 counts @23:26:58.04 Forearm.reconfigure :tight @23:26:58.10 Forearm.seek 3 + 450 counts @23:26:58.18 PS.seek 0ml @23:26:58.36 SE.seek :down @23:26:59.16 SE.stop @23:26:59.50 Forearm.reconfigure :loose @23:26:59.55 Forearm.seek :retract @23:27:00.50 Starting finalPurge @23:27:19.72 Waiting >20 seconds for psFlush to relinquish FlushPuck @23:27:24.67 PSV.dial PSR @23:27:25.53 PSR.select :flush @23:27:25.66 PS.seek 0.5ml @23:27:32.06 PSR.select :air @23:27:32.18 PS.seek 2.5ml @23:27:53.61 PSV.dial PTV @23:27:54.35 PBV.dial PSV @23:27:54.92 PS.seek 0ml @23:28:21.20 PSV.dial PBV @23:28:21.68 PS.seek 2.5ml @23:28:48.08 PSV.dial :waste1 @23:28:48.82 PS.seek 0ml @23:29:15.14 PSV.dial PSR @23:29:15.61 PSR.select :air @23:29:15.64 PS.seek 2.5ml @23:29:42.02 PSV.dial PTV @23:29:42.75 PBV.dial PSV @23:29:42.94 PS.seek 0ml @23:30:09.30 PSV.dial PBV @23:30:09.77 PS.seek 2.5ml @23:30:36.05 PSV.dial :waste1 @23:30:36.79 PS.seek 0ml @23:31:03.18
Loading FlushPuck into Collection Clamp from Processing Clamp @23:31:03.36 Elbow.seek PC @23:31:05.88 Elbow.reconfigure :loose @23:31:05.98 Forearm.seek PC @23:31:06.93 Hand.close @23:31:07.57 PC.seek :open @23:31:12.29 Forearm.seek :retract @23:31:13.26 Elbow.reconfigure :tight @23:31:13.31 Elbow.seek CC @23:31:16.96 Elbow.reconfigure :loose @23:31:17.05 Forearm.reconfigure :tight @23:31:17.10 Forearm.seek CC @23:31:18.31 CC.seek :closed @23:31:22.84 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.47 @23:31:22.91 Hand.open @23:31:23.64 Forearm.reconfigure :loose @23:31:23.69 Forearm.seek :retract @23:31:24.71 Flushing the Collection Valve::Manifold @23:31:24.83 CSV.dial CSR @23:31:25.27 Pulling 5ml of CSR.flush @23:31:25.28 CSR.select :flush @23:31:25.40 CS.seek 5ml @23:31:38.99 delay 5 seconds @23:31:44.01 CSR.select :air @23:31:44.18 CS.reconfigure :fast @23:31:44.23 CS.seek 7.5ml @23:31:50.99 CSV.dial :waste @23:31:52.29 CS.reconfigure :default @23:31:52.34 CS.seek 0ml @23:32:12.43 Flushing lower manifold's waste1 port @23:32:12.56 CSV.dial :flush @23:32:13.00 CS.reconfigure :fast @23:32:13.05 CS.seek 5ml @23:32:25.77 delay 5 seconds @23:32:30.79 CSV.dial :air @23:32:31.43 CS.seek 7.001ml @23:32:37.02 CSV.dial CTV @23:32:37.07
CTV.dial CSV @23:32:38.43 CBV.dial :waste1 @23:32:39.20 CS.seek 0ml @23:32:55.87 Flushing lower manifold's waste2 port @23:32:55.99 CSV.dial :flush @23:32:56.73 CS.seek 5ml @23:33:09.62 delay 5 seconds @23:33:14.64 CSV.dial :air @23:33:15.16 CS.seek 7ml @23:33:20.65 CSV.dial CTV @23:33:20.67
CTV.dial CSV @23:33:21.18 CBV.dial :waste2 @23:33:22.23 CS.seek 0ml @23:33:38.80 Flushing lower manifold's waste2b port @23:33:38.92 CSV.dial :flush @23:33:39.67 CS.seek 5ml @23:33:52.36 delay 5 seconds @23:33:57.38 CSV.dial :air @23:33:57.90 CS.seek 7ml @23:34:03.37 CSV.dial CTV @23:34:03.39
CTV.dial CSV @23:34:03.85 CBV.dial :waste2b @23:34:04.90 CS.seek 0ml @23:34:21.40 Disinfecting external Intake with kill @23:34:21.53 CSV.dial CSR @23:34:21.96 Pulling 8ml of CSR.kill @23:34:21.97 CSR.select :kill @23:34:22.09 CS.seek 8ml @23:34:41.93 delay 5 seconds @23:34:46.95 CSR.select :air @23:34:47.06 CS.seek 10ml @23:34:52.56 CSV.dial CBV @23:34:52.58
CBV.dial CSV @23:34:53.65 CTV.dial :intake @23:34:54.43 Intake.select :open @23:34:54.54 CS.seek 2ml @23:35:13.42 Intake.select :close @23:35:13.53 CTV.dial :air @23:35:14.29 CS.reconfigure :default @23:35:14.34 CS.seek 0ml @23:35:20.30 CSV.dial CSR @23:35:21.32 Pulling 5ml of CSR.flush @23:35:21.33 CSR.select :flush @23:35:21.45 CS.seek 5ml @23:35:35.18 delay 5 seconds @23:35:40.19 CSR.select :air @23:35:40.31 CS.seek 10ml @23:35:54.02 CSV.dial CBV @23:35:55.04 CS.seek 0ml @23:36:21.68 Garaging FlushPuck in Collection Clamp @23:36:21.75 Elbow.reconfigure :tight @23:36:21.80 Elbow.seek CC @23:36:22.11 Elbow.reconfigure :loose @23:36:22.21 Forearm.seek CC @23:36:23.27 Hand.close @23:36:23.94 CC.seek :open @23:36:28.58 Forearm.seek :retract @23:36:29.58 Elbow.reconfigure :tight @23:36:29.63 Elbow.seek :garage @23:36:32.56 Elbow.reconfigure :loose @23:36:32.66 Forearm.reconfigure :tight @23:36:33.13 Forearm.seek :garage @23:36:34.13 Hand.open @23:36:34.75 Forearm.reconfigure :loose @23:36:34.80 Forearm.seek :retract @23:36:35.75 Completed [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] begun 20:56:14 @23:36:35.81 Asleep after completing DA_WCR_113023b assays @23:36:35.83 GoodNews.email "{1 => 33,\n 2 => 33,\n 3 => 30,\n 4 => 3,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@23:35:37, 30% flow, 17.1C, 30% humidity, 15.2psia, 13.691V, 0.478A, 119.054Ah, 6.54W\nSampled 600.0ml",Subject: "Asleep after completing DA_WCR_113023b assays" @23:36:39.71 upsync + ESPwhich DA_WCR_120123.rb + fn=DA_WCR_120123.rb + IFS=: + set /home/esp/esp2/mission . + fullPath=/home/esp/esp2/mission/DA_WCR_120123.rb + [ -f /home/esp/esp2/mission/DA_WCR_120123.rb ] + realpath /home/esp/esp2/mission/DA_WCR_120123.rb + return 0 + scriptPath=/home/esp/esp2local/mission/DA_WCR_120123.rb + exec ruby /home/esp/esp2/lib/esp.rb /home/esp/esp2local/mission/DA_WCR_120123.rb @13:38:23.98UTC01-Dec-23
ESP2 v3.42 I2C Gateway v3.20 for core bus @13:38:26.58 Configuration for short puck jake 1/11/21 by brent@mbari.org /home/esp/esp2/type/shallow/jake/configure.rb @13:38:30.19 Polling Can every 10:00 @13:38:30.32 Cleanly exited at 13:34:18UTC01-Dec-23 @13:38:30.37 Loading /home/esp/esp2local/mission/DA_WCR_120123.rb mission @13:38:30.43 GoodNews.email "Starting DA_WCR_120123 w/pucks in tube 4\n{1 => 33,\n 2 => 33,\n 3 => 30,\n 4 => 3,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@13:38:30, 30% flow, 16.7C, 29% humidity, 15.1psia, 13.744V, 0.296A, 122.813Ah, 4.07W",Subject: "DA_WCR_120123 begun" @13:38:30.47
Preparing to sleep until + 00:00:05 @13:38:30.50 Asleep til + 00:00:05 @13:38:30.52 Continuing at 13:38 @13:38:30.80 Gate.power :core,:OFF @13:38:30.81 Gate.power :raw,:OFF @13:38:30.82 Shutdown @13:38:33.32 GoodNews.email "Then, in tube 4: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_120123.rb:14\n{1 => 33,\n 2 => 33,\n 3 => 30,\n 4 => 3,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@13:38:30, 30% flow, 16.7C, 29% humidity, 15.1psia, 13.744V, 0.296A, 122.813Ah, 4.07W",Subject: "Asleep until 13:38" @13:38:34.63 upsync @13:38:35.41
Powering core bus @13:38:37.24 upsync @13:38:40.85
Gate.power :raw,:ON @13:38:41.49 CS.configure :default @13:38:41.54 PS.configure :default @13:38:41.59 SS.configure :default @13:38:41.63 CC.configure :default @13:38:41.68 PC.configure :default @13:38:41.75 Forearm.configure :loose @13:38:41.80 Elbow.configure :loose @13:38:41.86 SC.configure :loose @13:38:41.91 SE.configure :default @13:38:41.96 CH.configure :default @13:38:41.99 PH.configure :default @13:38:42.03 All dwarf controllers running firmware version 3.85 @13:38:42.14 Valve.reset @13:38:42.36 Intake.select :close @13:38:42.39 Exhaust.select :close @13:38:42.44 CTV.dial :air @13:38:42.56 PSV.dial :air @13:38:42.59 CSV.dial :waste1 @13:38:42.73
Forearm.home.seek :retract @13:38:43.12 SSV.dial :spit,avoiding: :exhaust @13:38:43.54 Hand.close @13:38:43.57
Elbow.reconfigure :loose @13:38:43.66 Elbow.jog 1500 @13:38:43.81 PS.home.seek 0ml @13:38:44.00 CS.reconfigure :fast @13:38:44.03 SS.home.seek 0ml @13:38:44.14 CS.home.seek 0ml @13:38:44.22
Elbow.reconfigure :loose @13:38:44.28 Elbow.home.seek :clear @13:38:47.66 SE.home.seek :down @13:38:48.27 SC.reconfigure :tight @13:38:48.32 SC.home.seek :initial @13:38:52.06 PS.stop @13:39:04.03
SC.configure :limp @13:39:04.08 Forearm.seek :retract @13:39:04.15 Elbow.seek :hammer @13:39:05.19 Forearm.reconfigure :tight @13:39:05.24 Forearm.seek retracted @13:39:05.82 CC.home.seek :open @13:39:05.90 PC.home.seek :open @13:39:09.32
Phase [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] @13:39:09.33 Notice.email "At 13:39:09 began in tube 4: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_120123.rb:14\n{1 => 33,\n 2 => 33,\n 3 => 30,\n 4 => 3,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@13:39:09, 31% flow, 16.7C, 29% humidity, 15.1psia, 13.730V, 0.309A, 122.817Ah, 4.24W",Subject: "Started DA_WCR_120123 phase initialPurge; da [1000, 1000] #da and wsr are run simultanelously" @13:39:09.36
Starting initialPurge @13:39:09.38 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @13:39:09.45 Hand.open @13:39:10.24 Elbow.reconfigure :tight @13:39:10.29 Elbow.seek FlushPuck::Garage @13:39:12.13 Elbow.reconfigure :loose @13:39:12.23 Forearm.reconfigure :loose @13:39:12.28 Forearm.seek :garage @13:39:13.23 Hand.close @13:39:13.79 upsync @13:39:13.98
Forearm.seek :retract @13:39:15.00 Elbow.reconfigure :tight @13:39:15.04 Elbow.seek CC @13:39:18.03 Elbow.reconfigure :loose @13:39:18.14 Forearm.reconfigure :tight @13:39:18.19 Forearm.seek CC @13:39:19.52 CC.seek :closed @13:39:24.15 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.44 @13:39:24.22 Hand.open @13:39:24.91 Forearm.reconfigure :loose @13:39:24.96 Forearm.seek :retract @13:39:25.92 Flushing the Collection Valve::Manifold @13:39:26.04 CSV.dial CSR @13:39:27.33 Pulling 5ml of CSR.flush @13:39:27.34 CSR.select :flush @13:39:27.46 CS.reconfigure :default @13:39:27.91 CS.seek 5ml @13:39:41.77 delay 5 seconds @13:39:46.79 CSR.select :air @13:39:46.96 CS.reconfigure :fast @13:39:47.01 CS.seek 7.5ml @13:39:53.96 CSV.dial :waste @13:39:55.25 CS.reconfigure :default @13:39:55.29 CS.seek 0ml @13:40:15.24 Purging external Intake @13:40:15.36 CSV.dial :flush @13:40:15.82 CS.reconfigure :fast @13:40:15.87 CS.seek 10ml @13:40:40.21 delay 5 seconds @13:40:45.23 CSV.dial CBV @13:40:45.26
CBV.dial CSV @13:40:46.00 CTV.dial :intake @13:40:46.78 Intake.select :open @13:40:46.90 CS.seek 0ml @13:41:10.42 Intake.select :close @13:41:10.53 CTV.dial :air @13:41:11.30 Flushing upper manifold @13:41:11.42 CSV.dial :flush @13:41:12.17 CS.seek 5ml @13:41:25.05 delay 5 seconds @13:41:30.07 CSV.dial :air @13:41:30.60 CS.seek 7ml @13:41:36.11 CSV.dial CBV @13:41:36.13
CBV.dial CSV @13:41:37.18 CTV.dial :air @13:41:37.26 CS.seek 0ml @13:41:54.14 delay 7 seconds @13:42:01.17 CS.seek 3ml @13:42:09.20 CSV.dial :waste @13:42:09.66 CS.seek 0ml @13:42:17.45 CSV.dial :flush @13:42:17.90 CS.seek 3ml @13:42:25.89 delay 5 seconds @13:42:30.91 CSV.dial :air @13:42:31.41 CS.seek 5ml @13:42:37.14 CSV.dial :air @13:42:37.23 CS.seek 0ml @13:42:49.39 delay 7 seconds @13:42:56.42 CS.seek 3ml @13:43:04.45 CSV.dial :waste @13:43:05.19 CS.seek 0ml @13:43:12.83 Garaging FlushPuck in Collection Clamp @13:43:12.90 Elbow.reconfigure :tight @13:43:12.95 Elbow.seek CC @13:43:13.23 Elbow.reconfigure :loose @13:43:13.34 Forearm.seek CC @13:43:14.38 Hand.close @13:43:14.98 CC.seek :open @13:43:20.06 Forearm.seek :retract @13:43:21.10 Elbow.reconfigure :tight @13:43:21.15 Elbow.seek :garage @13:43:24.12 Elbow.reconfigure :loose @13:43:24.22 Forearm.reconfigure :tight @13:43:24.27 Forearm.seek :garage @13:43:25.25 Hand.open @13:43:25.91 Forearm.reconfigure :loose @13:43:25.95 Forearm.seek :retract @13:43:26.90 da sampling at most 1000ml, wcr at most 1000ml @13:43:26.93 Loading sh1 puck from tube 4 into Collection Clamp @13:43:26.95 DAfans.email "Started da in tube 4 at 13:43:26UTC01-Dec-23\n{1 => 33,\n 2 => 33,\n 3 => 30,\n 4 => 3,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@13:39:09, 31% flow, 16.7C, 29% humidity, 15.1psia, 13.730V, 0.309A, 122.817Ah, 4.24W",Subject: "da sampling at most 1000ml, wcr at most 1000ml" @13:43:27.23
SC.reconfigure :tight @13:43:27.29 SC.seek 4 @13:43:27.39 SC.configure :limp @13:43:27.65 Elbow.reconfigure :tight @13:43:27.70 Elbow.seek 4 @13:43:29.68 Forearm.reconfigure :tight @13:43:29.73 Forearm.seek 4 + 450 counts @13:43:30.80 SE.reconfigure :pick @13:43:30.87 SE.seek :beamBreak @13:43:31.10 upsync @13:43:33.88
SE.configure :default @13:43:34.05 Elbow.seek 4 @13:43:34.25 Forearm.seek 4 + 300 counts @13:43:34.47 Forearm.reconfigure :loose @13:43:34.52 Hand.close @13:43:35.11 SE.seek :down @13:43:35.32
Forearm.seek :retract @13:43:36.77 Elbow.seek CC @13:43:37.78 SE.stop @13:43:38.44
Elbow.reconfigure :loose @13:43:38.54 Forearm.reconfigure :tight @13:43:38.59 Forearm.seek CC @13:43:39.89 CC.seek :closed @13:43:44.48 Collection Clamp onSpring/offSpring for 14/27 samples, on/off torque 2.23 @13:43:44.49 Hand.open @13:43:45.16 Forearm.reconfigure :loose @13:43:45.20 Forearm.seek :retract @13:43:46.17 collectSample 1000ml w/bubble point of 24psi @13:43:46.18 Priming 7ml @13:43:46.19 Intake.select :close @13:43:46.20 Exhaust.select :close @13:43:46.22 SS.hold @13:43:46.43 Calibrating pressure and temperature sensors @13:43:46.46 CTV.dial :air @13:43:46.48 SSV.dial :spit,avoiding: :exhaust @13:43:46.57 SS.reconfigure :fast @13:43:46.61 SS.seek 5ml @13:43:49.48 SSV.dial CBV @13:43:49.51
CBV.dial SSV @13:43:50.84 delay 3.8 seconds @13:43:54.77 Sensor limits: Intake max 103.0psia; Outlet max 95.7psia @13:43:54.78 SSV.dial :spit @13:43:56.10 SS.seek 0ml @13:43:58.81 SSV.dial CBV @13:44:00.14 CTV.dial :intake @13:44:00.92 SSV.dial CBV @13:44:00.95
CBV.dial SSV @13:44:01.15 Allowing 40s for 7.0ml of remaining 7.0ml @13:44:01.16 Intake.select :open @13:44:03.33 Intake @14.4psia, vacuum from 8.9 to 11.9psi @13:44:03.35 SS.reconfigure :inhaling 2.20ml/s @13:44:03.40 SS.seek 7ml @13:44:10.20 Stroke completed -- waiting for pressure to equalize ... @13:44:10.21 SS.reconfigure :exhale @13:44:10.25 SS.hold @13:44:15.34 Intake.select :close @13:44:15.50 SSV.dial :exhaust @13:44:16.26 Exhaust.select :open @13:44:16.38 SS.seek 0ml @13:44:20.38 Exhaust.select :close @13:44:20.48 Sampling 1000.0ml @13:44:20.49 Intake.select :close @13:44:20.50 Exhaust.select :close @13:44:20.52 SS.reconfigure :default @13:44:20.56 SS.hold @13:44:20.76 CTV.dial :intake @13:44:20.94 SSV.dial CBV @13:44:20.96
CBV.dial SSV @13:44:21.73 Allowing 130s for 25.0ml of remaining 1000.0ml @13:44:21.74 Intake.select :open @13:44:23.91 Intake @15.3psia, vacuum from 9.8 to 12.8psi @13:44:23.92 SS.reconfigure :inhaling 2.20ml/s @13:44:23.97 SS.seek 25ml @13:44:38.96 Vacuum too high -- waiting up to 40 seconds @13:44:38.97 SS.reconfigure :exhale @13:44:39.02 SS.hold @13:44:43.27 Intake @15.6psia, vacuum from 10.0 to 13.0psi @13:44:43.29 SS.reconfigure :inhaling 1.76ml/s @13:44:43.34 SS.seek 25ml @13:44:43.87 Stroke completed -- waiting for pressure to equalize ... @13:44:43.88 SS.reconfigure :exhale @13:44:43.92 SS.hold @13:45:06.81 Intake.select :close @13:45:06.97 SSV.dial :exhaust @13:45:07.73 Exhaust.select :open @13:45:07.84 SS.seek 0ml @13:45:16.70 Exhaust.select :close @13:45:16.80 SSV.dial CBV @13:45:16.82
CBV.dial SSV @13:45:17.59 Allowing 130s for 25.0ml of remaining 975.0ml @13:45:17.60 Intake.select :open @13:45:19.77 Intake @15.8psia, vacuum from 10.0 to 13.0psi @13:45:19.78 SS.reconfigure :inhaling 1.76ml/s @13:45:19.83 SS.seek 25ml @13:45:34.71 Vacuum too high -- waiting up to 40 seconds @13:45:34.72 SS.reconfigure :exhale @13:45:34.77 SS.hold @13:45:38.67 Intake @18.3psia, vacuum from 10.0 to 13.0psi @13:45:38.68 SS.reconfigure :inhaling 1.41ml/s @13:45:38.73 SS.seek 25ml @13:45:42.15 Stroke completed -- waiting for pressure to equalize ... @13:45:42.16 SS.reconfigure :exhale @13:45:42.20 SS.hold @13:46:08.65 Intake.select :close @13:46:08.81 SSV.dial :exhaust @13:46:09.58 Exhaust.select :open @13:46:09.69 SS.seek 0ml @13:46:19.10 Exhaust.select :close @13:46:19.21 SSV.dial CBV @13:46:19.23
CBV.dial SSV @13:46:20.01 Allowing 130s for 25.0ml of remaining 950.0ml @13:46:20.02 Intake.select :open @13:46:22.19 Intake @15.9psia, vacuum from 10.0 to 13.0psi @13:46:22.21 SS.reconfigure :inhaling 1.41ml/s @13:46:22.25 SS.seek 25ml @13:46:42.58 Stroke completed -- waiting for pressure to equalize ... @13:46:42.59 SS.reconfigure :exhale @13:46:42.64 SS.hold @13:47:09.18 Intake.select :close @13:47:09.33 SSV.dial :exhaust @13:47:10.09 Exhaust.select :open @13:47:10.21 SS.seek 0ml @13:47:19.02 Exhaust.select :close @13:47:19.13 SSV.dial CBV @13:47:19.16
CBV.dial SSV @13:47:19.99 Allowing 130s for 25.0ml of remaining 925.0ml @13:47:20.00 Intake.select :open @13:47:22.16 Intake @15.9psia, vacuum from 10.0 to 13.0psi @13:47:22.17 SS.reconfigure :inhaling 1.41ml/s @13:47:22.23 SS.seek 25ml @13:47:42.83 Stroke completed -- waiting for pressure to equalize ... @13:47:42.84 SS.reconfigure :exhale @13:47:42.89 SS.hold @13:48:03.32 Intake.select :close @13:48:03.47 SSV.dial :exhaust @13:48:04.23 Exhaust.select :open @13:48:04.34 SS.seek 0ml @13:48:13.02 Exhaust.select :close @13:48:13.13 SSV.dial CBV @13:48:13.15
CBV.dial SSV @13:48:13.93 Allowing 130s for 25.0ml of remaining 900.0ml @13:48:13.94 Intake.select :open @13:48:16.11 Intake @16.0psia, vacuum from 10.0 to 13.0psi @13:48:16.12 SS.reconfigure :inhaling 1.41ml/s @13:48:16.17 SS.seek 25ml @13:48:36.94 Stroke completed -- waiting for pressure to equalize ... @13:48:36.95 SS.reconfigure :exhale @13:48:37.00 SS.hold @13:48:57.62 Intake.select :close @13:48:57.78 SSV.dial :exhaust @13:48:58.54 Exhaust.select :open @13:48:58.65 SS.seek 0ml @13:49:07.41 Exhaust.select :close @13:49:07.52 SSV.dial CBV @13:49:07.54
CBV.dial SSV @13:49:08.32 Allowing 130s for 25.0ml of remaining 875.0ml @13:49:08.33 Intake.select :open @13:49:10.50 Intake @15.9psia, vacuum from 10.0 to 13.0psi @13:49:10.51 SS.reconfigure :inhaling 1.41ml/s @13:49:10.56 SS.seek 25ml @13:49:31.03 Stroke completed -- waiting for pressure to equalize ... @13:49:31.04 SS.reconfigure :exhale @13:49:31.09 SS.hold @13:49:49.58 Intake.select :close @13:49:49.74 SSV.dial :exhaust @13:49:50.50 Exhaust.select :open @13:49:50.62 SS.seek 0ml @13:49:59.27 Exhaust.select :close @13:49:59.38 SSV.dial CBV @13:49:59.40
CBV.dial SSV @13:50:00.18 Allowing 130s for 25.0ml of remaining 850.0ml @13:50:00.19 Intake.select :open @13:50:02.35 Intake @15.9psia, vacuum from 10.0 to 13.0psi @13:50:02.37 SS.reconfigure :inhaling 1.41ml/s @13:50:02.41 SS.seek 25ml @13:50:23.03 Stroke completed -- waiting for pressure to equalize ... @13:50:23.04 SS.reconfigure :exhale @13:50:23.09 SS.hold @13:50:39.39 Intake.select :close @13:50:39.55 SSV.dial :exhaust @13:50:40.33 Exhaust.select :open @13:50:40.45 SS.seek 0ml @13:50:49.41 Exhaust.select :close @13:50:49.52 SSV.dial CBV @13:50:49.54
CBV.dial SSV @13:50:50.32 Allowing 130s for 25.0ml of remaining 825.0ml @13:50:50.33 Intake.select :open @13:50:52.49 Intake @15.9psia, vacuum from 10.0 to 13.0psi @13:50:52.51 SS.reconfigure :inhaling 1.41ml/s @13:50:52.56 SS.seek 25ml @13:51:12.62 Stroke completed -- waiting for pressure to equalize ... @13:51:12.63 SS.reconfigure :exhale @13:51:12.68 SS.hold @13:51:29.01 Intake.select :close @13:51:29.16 SSV.dial :exhaust @13:51:29.92 Exhaust.select :open @13:51:30.04 SS.seek 0ml @13:51:38.80 Exhaust.select :close @13:51:38.91 SSV.dial CBV @13:51:38.93
CBV.dial SSV @13:51:39.71 Allowing 130s for 25.0ml of remaining 800.0ml @13:51:39.72 Intake.select :open @13:51:41.89 Intake @15.9psia, vacuum from 10.0 to 13.0psi @13:51:41.90 SS.reconfigure :inhaling 1.41ml/s @13:51:41.95 SS.seek 25ml @13:52:02.14 Stroke completed -- waiting for pressure to equalize ... @13:52:02.15 SS.reconfigure :exhale @13:52:02.20 SS.hold @13:52:18.45 Intake.select :close @13:52:18.61 SSV.dial :exhaust @13:52:19.36 Exhaust.select :open @13:52:19.90 SS.seek 0ml @13:52:28.75 Exhaust.select :close @13:52:28.86 SSV.dial CBV @13:52:28.88
CBV.dial SSV @13:52:29.66 Allowing 130s for 25.0ml of remaining 775.0ml @13:52:29.67 Intake.select :open @13:52:31.85 Intake @15.8psia, vacuum from 10.0 to 13.0psi @13:52:31.87 SS.reconfigure :inhaling 1.41ml/s @13:52:31.91 SS.seek 25ml @13:52:52.06 Stroke completed -- waiting for pressure to equalize ... @13:52:52.07 SS.reconfigure :exhale @13:52:52.12 SS.hold @13:53:06.49 Intake.select :close @13:53:06.65 SSV.dial :exhaust @13:53:07.42 Exhaust.select :open @13:53:07.54 SS.seek 0ml @13:53:16.14 Exhaust.select :close @13:53:16.25 SSV.dial CBV @13:53:16.27
CBV.dial SSV @13:53:17.05 Allowing 130s for 25.0ml of remaining 750.0ml @13:53:17.06 Intake.select :open @13:53:19.22 Intake @15.9psia, vacuum from 10.0 to 13.0psi @13:53:19.24 SS.reconfigure :inhaling 1.41ml/s @13:53:19.28 SS.seek 25ml @13:53:39.68 Stroke completed -- waiting for pressure to equalize ... @13:53:39.69 SS.reconfigure :exhale @13:53:39.74 SS.hold @13:53:52.68 Intake.select :close @13:53:52.83 SSV.dial :exhaust @13:53:53.59 Exhaust.select :open @13:53:53.71 SS.seek 0ml @13:54:02.36 Exhaust.select :close @13:54:02.46 SSV.dial CBV @13:54:02.48
CBV.dial SSV @13:54:03.25 Allowing 130s for 25.0ml of remaining 725.0ml @13:54:03.26 Intake.select :open @13:54:05.43 Intake @15.9psia, vacuum from 10.0 to 13.0psi @13:54:05.44 SS.reconfigure :inhaling 1.41ml/s @13:54:05.49 SS.seek 25ml @13:54:25.79 Stroke completed -- waiting for pressure to equalize ... @13:54:25.80 SS.reconfigure :exhale @13:54:25.85 SS.hold @13:54:40.22 Intake.select :close @13:54:40.38 SSV.dial :exhaust @13:54:41.14 Exhaust.select :open @13:54:41.25 SS.seek 0ml @13:54:49.97 Exhaust.select :close @13:54:50.08 SSV.dial CBV @13:54:50.10
CBV.dial SSV @13:54:50.87 Allowing 130s for 25.0ml of remaining 700.0ml @13:54:50.88 Intake.select :open @13:54:53.05 Intake @15.7psia, vacuum from 10.0 to 13.0psi @13:54:53.07 SS.reconfigure :inhaling 1.41ml/s @13:54:53.11 SS.seek 25ml @13:55:13.76 Stroke completed -- waiting for pressure to equalize ... @13:55:13.77 SS.reconfigure :exhale @13:55:13.82 SS.hold @13:55:26.48 Intake.select :close @13:55:26.63 SSV.dial :exhaust @13:55:27.39 Exhaust.select :open @13:55:27.50 SS.seek 0ml @13:55:36.11 Exhaust.select :close @13:55:36.21 SSV.dial CBV @13:55:36.23
CBV.dial SSV @13:55:37.01 Allowing 130s for 25.0ml of remaining 675.0ml @13:55:37.03 Intake.select :open @13:55:39.19 Intake @16.0psia, vacuum from 10.0 to 13.0psi @13:55:39.21 SS.reconfigure :inhaling 1.41ml/s @13:55:39.26 SS.seek 25ml @13:55:59.78 Stroke completed -- waiting for pressure to equalize ... @13:55:59.79 SS.reconfigure :exhale @13:55:59.83 SS.hold @13:56:14.12 Intake.select :close @13:56:14.27 SSV.dial :exhaust @13:56:15.03 Exhaust.select :open @13:56:15.14 SS.seek 0ml @13:56:23.78 Exhaust.select :close @13:56:23.89 SSV.dial CBV @13:56:23.92
CBV.dial SSV @13:56:24.67 Allowing 130s for 25.0ml of remaining 650.0ml @13:56:24.68 Intake.select :open @13:56:26.84 Intake @15.8psia, vacuum from 10.0 to 13.0psi @13:56:26.86 SS.reconfigure :inhaling 1.41ml/s @13:56:26.91 SS.seek 25ml @13:56:48.17 Stroke completed -- waiting for pressure to equalize ... @13:56:48.18 SS.reconfigure :exhale @13:56:48.22 SS.hold @13:57:02.59 Intake.select :close @13:57:02.75 SSV.dial :exhaust @13:57:03.51 Exhaust.select :open @13:57:03.62 SS.seek 0ml @13:57:12.15 Exhaust.select :close @13:57:12.26 SSV.dial CBV @13:57:12.28
CBV.dial SSV @13:57:13.06 Allowing 130s for 25.0ml of remaining 625.0ml @13:57:13.07 Intake.select :open @13:57:15.24 Intake @15.8psia, vacuum from 10.0 to 13.0psi @13:57:15.25 SS.reconfigure :inhaling 1.41ml/s @13:57:15.30 SS.seek 25ml @13:57:35.84 Stroke completed -- waiting for pressure to equalize ... @13:57:35.85 SS.reconfigure :exhale @13:57:35.89 SS.hold @13:57:50.28 Intake.select :close @13:57:50.43 SSV.dial :exhaust @13:57:51.20 Exhaust.select :open @13:57:51.31 SS.seek 0ml @13:57:59.90 Exhaust.select :close @13:58:00.01 SSV.dial CBV @13:58:00.03
CBV.dial SSV @13:58:00.81 Allowing 130s for 25.0ml of remaining 600.0ml @13:58:00.82 Intake.select :open @13:58:02.98 Intake @16.0psia, vacuum from 10.0 to 13.0psi @13:58:03.00 SS.reconfigure :inhaling 1.41ml/s @13:58:03.04 SS.seek 25ml @13:58:23.48 Stroke completed -- waiting for pressure to equalize ... @13:58:23.49 SS.reconfigure :exhale @13:58:23.53 SS.hold @13:58:39.83 Intake.select :close @13:58:40.04 SSV.dial :exhaust @13:58:40.82 Exhaust.select :open @13:58:40.94 SS.seek 0ml @13:58:49.71 Exhaust.select :close @13:58:49.82 SSV.dial CBV @13:58:49.84
CBV.dial SSV @13:58:50.62 Allowing 130s for 25.0ml of remaining 575.0ml @13:58:50.63 Intake.select :open @13:58:52.80 Intake @15.8psia, vacuum from 10.0 to 13.0psi @13:58:52.81 SS.reconfigure :inhaling 1.41ml/s @13:58:52.86 SS.seek 25ml @13:59:13.65 Stroke completed -- waiting for pressure to equalize ... @13:59:13.66 SS.reconfigure :exhale @13:59:13.71 SS.hold @13:59:29.95 Intake.select :close @13:59:30.11 SSV.dial :exhaust @13:59:30.88 Exhaust.select :open @13:59:31.00 SS.seek 0ml @13:59:39.76 Exhaust.select :close @13:59:39.86 SSV.dial CBV @13:59:39.89
CBV.dial SSV @13:59:40.67 Allowing 130s for 25.0ml of remaining 550.0ml @13:59:40.68 Intake.select :open @13:59:42.84 Intake @15.8psia, vacuum from 10.0 to 13.0psi @13:59:42.85 SS.reconfigure :inhaling 1.41ml/s @13:59:42.90 SS.seek 25ml @14:00:03.50 Stroke completed -- waiting for pressure to equalize ... @14:00:03.52 SS.reconfigure :exhale @14:00:03.56 SS.hold @14:00:21.90 Intake.select :close @14:00:22.06 SSV.dial :exhaust @14:00:22.82 Exhaust.select :open @14:00:22.93 SS.seek 0ml @14:00:31.67 Exhaust.select :close @14:00:31.78 SSV.dial CBV @14:00:31.80
CBV.dial SSV @14:00:32.57 Allowing 130s for 25.0ml of remaining 525.0ml @14:00:32.58 Intake.select :open @14:00:34.75 Intake @15.7psia, vacuum from 10.0 to 13.0psi @14:00:34.77 SS.reconfigure :inhaling 1.41ml/s @14:00:34.81 SS.seek 25ml @14:00:55.32 Stroke completed -- waiting for pressure to equalize ... @14:00:55.33 SS.reconfigure :exhale @14:00:55.37 SS.hold @14:01:13.72 Intake.select :close @14:01:13.88 SSV.dial :exhaust @14:01:14.66 Exhaust.select :open @14:01:14.78 SS.seek 0ml @14:01:23.52 Exhaust.select :close @14:01:23.63 SSV.dial CBV @14:01:23.65
CBV.dial SSV @14:01:24.44 Allowing 130s for 25.0ml of remaining 500.0ml @14:01:24.46 Intake.select :open @14:01:26.62 Intake @16.0psia, vacuum from 10.0 to 13.0psi @14:01:26.64 SS.reconfigure :inhaling 1.41ml/s @14:01:26.69 SS.seek 25ml @14:01:45.72 Vacuum too high -- waiting up to 40 seconds @14:01:45.73 SS.reconfigure :exhale @14:01:45.78 SS.hold @14:01:55.74 Intake @17.1psia, vacuum from 10.0 to 13.0psi @14:01:55.76 SS.reconfigure :inhaling 1.13ml/s @14:01:55.80 SS.seek 25ml @14:01:58.35 Stroke completed -- waiting for pressure to equalize ... @14:01:58.36 SS.reconfigure :exhale @14:01:58.40 SS.hold @14:02:07.38 Intake.select :close @14:02:07.54 SSV.dial :exhaust @14:02:08.30 Exhaust.select :open @14:02:08.42 SS.seek 0ml @14:02:17.19 Exhaust.select :close @14:02:17.30 SSV.dial CBV @14:02:17.32
CBV.dial SSV @14:02:18.12 Allowing 130s for 25.0ml of remaining 475.0ml @14:02:18.13 Intake.select :open @14:02:20.30 Intake @15.9psia, vacuum from 10.0 to 13.0psi @14:02:20.31 SS.reconfigure :inhaling 1.13ml/s @14:02:20.36 SS.seek 25ml @14:02:45.10 Stroke completed -- waiting for pressure to equalize ... @14:02:45.11 SS.reconfigure :exhale @14:02:45.16 SS.hold @14:03:03.53 Intake.select :close @14:03:03.69 SSV.dial :exhaust @14:03:04.85 Exhaust.select :open @14:03:04.96 SS.seek 0ml @14:03:13.85 Exhaust.select :close @14:03:13.95 SSV.dial CBV @14:03:13.97
CBV.dial SSV @14:03:14.76 Allowing 130s for 25.0ml of remaining 450.0ml @14:03:14.77 Intake.select :open @14:03:16.93 Intake @15.8psia, vacuum from 10.0 to 13.0psi @14:03:16.95 SS.reconfigure :inhaling 1.13ml/s @14:03:16.99 SS.seek 25ml @14:03:41.41 Stroke completed -- waiting for pressure to equalize ... @14:03:41.42 SS.reconfigure :exhale @14:03:41.46 SS.hold @14:04:01.78 Intake.select :close @14:04:01.93 SSV.dial :exhaust @14:04:02.71 Exhaust.select :open @14:04:02.83 SS.seek 0ml @14:04:11.49 Exhaust.select :close @14:04:11.60 SSV.dial CBV @14:04:12.04
CBV.dial SSV @14:04:12.82 Allowing 130s for 25.0ml of remaining 425.0ml @14:04:12.83 Intake.select :open @14:04:14.99 Intake @15.8psia, vacuum from 10.0 to 13.0psi @14:04:15.01 SS.reconfigure :inhaling 1.13ml/s @14:04:15.05 SS.seek 25ml @14:04:40.02 Stroke completed -- waiting for pressure to equalize ... @14:04:40.03 SS.reconfigure :exhale @14:04:40.07 SS.hold @14:05:00.58 Intake.select :close @14:05:00.73 SSV.dial :exhaust @14:05:01.49 Exhaust.select :open @14:05:01.61 SS.seek 0ml @14:05:10.67 Exhaust.select :close @14:05:10.78 SSV.dial CBV @14:05:10.81
CBV.dial SSV @14:05:11.58 Allowing 130s for 25.0ml of remaining 400.0ml @14:05:11.59 Intake.select :open @14:05:13.76 Intake @15.9psia, vacuum from 10.0 to 13.0psi @14:05:13.78 SS.reconfigure :inhaling 1.13ml/s @14:05:13.82 SS.seek 25ml @14:05:38.17 Stroke completed -- waiting for pressure to equalize ... @14:05:38.18 SS.reconfigure :exhale @14:05:38.23 SS.hold @14:06:00.68 Intake.select :close @14:06:00.84 SSV.dial :exhaust @14:06:01.61 Exhaust.select :open @14:06:01.73 SS.seek 0ml @14:06:10.48 Exhaust.select :close @14:06:10.59 SSV.dial CBV @14:06:10.62
CBV.dial SSV @14:06:11.39 Allowing 130s for 25.0ml of remaining 375.0ml @14:06:11.40 Intake.select :open @14:06:13.57 Intake @15.9psia, vacuum from 10.0 to 13.0psi @14:06:13.59 SS.reconfigure :inhaling 1.13ml/s @14:06:13.63 SS.seek 25ml @14:06:34.30 Vacuum too high -- waiting up to 40 seconds @14:06:34.32 SS.reconfigure :exhale @14:06:34.37 SS.hold @14:06:47.41 Intake @17.8psia, vacuum from 10.0 to 13.0psi @14:06:47.43 SS.reconfigure :inhaling 0.91ml/s @14:06:47.48 SS.seek 25ml @14:06:52.78 Stroke completed -- waiting for pressure to equalize ... @14:06:52.79 SS.reconfigure :exhale @14:06:52.84 SS.hold @14:07:04.03 Intake.select :close @14:07:04.18 SSV.dial :exhaust @14:07:04.95 Exhaust.select :open @14:07:05.07 SS.seek 0ml @14:07:13.77 Exhaust.select :close @14:07:13.87 SSV.dial CBV @14:07:13.91
CBV.dial SSV @14:07:14.66 Allowing 130s for 25.0ml of remaining 350.0ml @14:07:14.67 Intake.select :open @14:07:16.84 Intake @15.8psia, vacuum from 10.0 to 13.0psi @14:07:16.85 SS.reconfigure :inhaling 0.91ml/s @14:07:16.90 SS.seek 25ml @14:07:46.79 Stroke completed -- waiting for pressure to equalize ... @14:07:46.80 SS.reconfigure :exhale @14:07:46.85 SS.hold @14:08:09.30 Intake.select :close @14:08:09.47 SSV.dial :exhaust @14:08:10.23 Exhaust.select :open @14:08:10.35 SS.seek 0ml @14:08:19.23 Exhaust.select :close @14:08:19.35 SSV.dial CBV @14:08:19.37
CBV.dial SSV @14:08:20.14 Allowing 130s for 25.0ml of remaining 325.0ml @14:08:20.15 Intake.select :open @14:08:22.32 Intake @15.9psia, vacuum from 10.0 to 13.0psi @14:08:22.33 SS.reconfigure :inhaling 0.91ml/s @14:08:22.39 SS.seek 25ml @14:08:50.30 Vacuum too high -- waiting up to 40 seconds @14:08:50.32 SS.reconfigure :exhale @14:08:50.37 SS.hold @14:09:04.89 Intake @17.7psia, vacuum from 10.0 to 13.0psi @14:09:04.91 SS.reconfigure :inhaling 0.73ml/s @14:09:04.95 SS.seek 25ml @14:09:07.00 Stroke completed -- waiting for pressure to equalize ... @14:09:07.01 SS.reconfigure :exhale @14:09:07.05 SS.hold @14:09:17.26 Intake.select :close @14:09:17.43 SSV.dial :exhaust @14:09:18.19 Exhaust.select :open @14:09:18.30 SS.seek 0ml @14:09:27.22 Exhaust.select :close @14:09:27.33 SSV.dial CBV @14:09:27.37
CBV.dial SSV @14:09:28.11 Allowing 130s for 25.0ml of remaining 300.0ml @14:09:28.12 Intake.select :open @14:09:30.29 Intake @15.8psia, vacuum from 10.0 to 13.0psi @14:09:30.30 SS.reconfigure :inhaling 0.73ml/s @14:09:30.35 SS.seek 25ml @14:10:06.21 Stroke completed -- waiting for pressure to equalize ... @14:10:06.22 SS.reconfigure :exhale @14:10:06.27 SS.hold @14:10:29.13 Intake.select :close @14:10:29.28 SSV.dial :exhaust @14:10:30.06 Exhaust.select :open @14:10:30.17 SS.seek 0ml @14:10:38.81 Exhaust.select :close @14:10:38.92 SSV.dial CBV @14:10:38.94
CBV.dial SSV @14:10:39.72 Allowing 130s for 25.0ml of remaining 275.0ml @14:10:39.73 Intake.select :open @14:10:41.89 Intake @16.0psia, vacuum from 10.0 to 13.0psi @14:10:41.91 SS.reconfigure :inhaling 0.73ml/s @14:10:41.95 SS.seek 25ml @14:11:18.34 Stroke completed -- waiting for pressure to equalize ... @14:11:18.35 SS.reconfigure :exhale @14:11:18.39 SS.hold @14:11:42.96 Intake.select :close @14:11:43.12 SSV.dial :exhaust @14:11:43.89 Exhaust.select :open @14:11:44.00 SS.seek 0ml @14:11:53.06 Exhaust.select :close @14:11:53.16 SSV.dial CBV @14:11:53.19
CBV.dial SSV @14:11:53.96 Allowing 130s for 25.0ml of remaining 250.0ml @14:11:53.98 Intake.select :open @14:11:56.14 Intake @16.0psia, vacuum from 10.0 to 13.0psi @14:11:56.16 SS.reconfigure :inhaling 0.73ml/s @14:11:56.21 SS.seek 25ml @14:12:32.30 Stroke completed -- waiting for pressure to equalize ... @14:12:32.32 SS.reconfigure :exhale @14:12:32.40 Ignoring excess vacuum after stroke's end ... @14:12:32.41 SS.reconfigure :exhale @14:12:32.46 SS.hold @14:12:59.10 Intake.select :close @14:12:59.25 SSV.dial :exhaust @14:13:00.03 Exhaust.select :open @14:13:00.15 SS.seek 0ml @14:13:09.18 Exhaust.select :close @14:13:09.29 SSV.dial CBV @14:13:09.31
CBV.dial SSV @14:13:10.09 Allowing 130s for 25.0ml of remaining 225.0ml @14:13:10.10 Intake.select :open @14:13:12.27 Intake @16.0psia, vacuum from 10.0 to 13.0psi @14:13:12.28 SS.reconfigure :inhaling 0.73ml/s @14:13:12.33 SS.seek 25ml @14:13:44.42 Vacuum too high -- waiting up to 40 seconds @14:13:44.43 SS.reconfigure :exhale @14:13:44.49 SS.hold @14:14:00.98 Intake @17.6psia, vacuum from 10.0 to 13.0psi @14:14:01.00 SS.reconfigure :inhaling 0.59ml/s @14:14:01.04 SS.seek 25ml @14:14:06.52 Stroke completed -- waiting for pressure to equalize ... @14:14:06.53 SS.reconfigure :exhale @14:14:06.58 SS.hold @14:14:20.97 Intake.select :close @14:14:21.12 SSV.dial :exhaust @14:14:22.32 Exhaust.select :open @14:14:22.44 SS.seek 0ml @14:14:31.16 Exhaust.select :close @14:14:31.27 SSV.dial CBV @14:14:31.30
CBV.dial SSV @14:14:32.07 Allowing 130s for 25.0ml of remaining 200.0ml @14:14:32.08 Intake.select :open @14:14:34.25 Intake @15.8psia, vacuum from 10.0 to 13.0psi @14:14:34.27 SS.reconfigure :inhaling 0.59ml/s @14:14:34.32 SS.seek 25ml @14:15:19.05 Stroke completed -- waiting for pressure to equalize ... @14:15:19.06 SS.reconfigure :exhale @14:15:19.11 SS.hold @14:15:45.69 Intake.select :close @14:15:46.26 SSV.dial :exhaust @14:15:47.05 Exhaust.select :open @14:15:47.17 SS.seek 0ml @14:15:55.99 Exhaust.select :close @14:15:56.10 SSV.dial CBV @14:15:56.12
CBV.dial SSV @14:15:56.90 Allowing 130s for 25.0ml of remaining 175.0ml @14:15:56.91 Intake.select :open @14:15:59.08 Intake @16.0psia, vacuum from 10.0 to 13.0psi @14:15:59.10 SS.reconfigure :inhaling 0.59ml/s @14:15:59.15 SS.seek 25ml @14:16:44.42 Stroke completed -- waiting for pressure to equalize ... @14:16:44.44 SS.reconfigure :exhale @14:16:44.48 SS.hold @14:17:15.12 Intake.select :close @14:17:15.27 SSV.dial :exhaust @14:17:16.04 Exhaust.select :open @14:17:16.15 SS.seek 0ml @14:17:25.22 Exhaust.select :close @14:17:25.33 SSV.dial CBV @14:17:25.36
CBV.dial SSV @14:17:26.13 Allowing 130s for 25.0ml of remaining 150.0ml @14:17:26.14 Intake.select :open @14:17:28.31 Intake @16.0psia, vacuum from 10.0 to 13.0psi @14:17:28.33 SS.reconfigure :inhaling 0.59ml/s @14:17:28.37 SS.seek 25ml @14:18:12.39 Vacuum too high -- waiting up to 40 seconds @14:18:12.40 SS.reconfigure :exhale @14:18:12.45 SS.hold @14:18:33.00 Intake @16.9psia, vacuum from 10.0 to 13.0psi @14:18:33.01 SS.reconfigure :inhaling 0.47ml/s @14:18:33.06 SS.seek 25ml @14:18:34.38 Stroke completed -- waiting for pressure to equalize ... @14:18:34.39 SS.reconfigure :exhale @14:18:34.43 SS.hold @14:18:48.73 Intake.select :close @14:18:48.88 SSV.dial :exhaust @14:18:49.66 Exhaust.select :open @14:18:49.77 SS.seek 0ml @14:18:58.58 Exhaust.select :close @14:18:58.69 SSV.dial CBV @14:18:58.71
CBV.dial SSV @14:18:59.50 Allowing 130s for 25.0ml of remaining 125.0ml @14:18:59.51 Intake.select :open @14:19:01.68 Intake @15.9psia, vacuum from 10.0 to 13.0psi @14:19:01.69 SS.reconfigure :inhaling 0.47ml/s @14:19:01.74 SS.seek 25ml @14:19:57.30 Stroke completed -- waiting for pressure to equalize ... @14:19:57.31 SS.reconfigure :exhale @14:19:57.35 SS.hold @14:20:28.55 Intake.select :close @14:20:28.71 SSV.dial :exhaust @14:20:29.47 Exhaust.select :open @14:20:29.58 SS.seek 0ml @14:20:38.58 Exhaust.select :close @14:20:38.69 SSV.dial CBV @14:20:38.71
CBV.dial SSV @14:20:39.49 Allowing 130s for 25.0ml of remaining 99.9ml @14:20:39.50 Intake.select :open @14:20:41.66 Intake @16.0psia, vacuum from 10.0 to 13.0psi @14:20:41.67 SS.reconfigure :inhaling 0.47ml/s @14:20:41.72 SS.seek 25ml @14:21:37.12 Stroke completed -- waiting for pressure to equalize ... @14:21:37.13 SS.reconfigure :exhale @14:21:37.18 SS.hold @14:22:14.24 Intake.select :close @14:22:14.39 SSV.dial :exhaust @14:22:15.16 Exhaust.select :open @14:22:15.27 SS.seek 0ml @14:22:24.15 Exhaust.select :close @14:22:24.26 SSV.dial CBV @14:22:24.29
CBV.dial SSV @14:22:25.05 Allowing 130s for 25.0ml of remaining 74.9ml @14:22:25.06 Intake.select :open @14:22:27.60 Intake @16.0psia, vacuum from 10.0 to 13.0psi @14:22:27.61 SS.reconfigure :inhaling 0.47ml/s @14:22:27.66 SS.seek 25ml @14:23:20.63 Vacuum too high -- waiting up to 40 seconds @14:23:20.64 SS.reconfigure :exhale @14:23:20.70 SS.hold @14:23:45.32 Intake @18.0psia, vacuum from 10.0 to 13.0psi @14:23:45.33 SS.reconfigure :inhaling 0.38ml/s @14:23:45.38 SS.seek 25ml @14:23:48.47 Stroke completed -- waiting for pressure to equalize ... @14:23:48.48 SS.reconfigure :exhale @14:23:48.53 SS.hold @14:24:05.07 Intake.select :close @14:24:05.22 SSV.dial :exhaust @14:24:06.00 Exhaust.select :open @14:24:06.11 SS.seek 0ml @14:24:14.93 Exhaust.select :close @14:24:15.04 SSV.dial CBV @14:24:15.06
CBV.dial SSV @14:24:15.84 Allowing 130s for 25.0ml of remaining 49.9ml @14:24:15.85 Intake.select :open @14:24:18.07 Intake @16.1psia, vacuum from 10.0 to 13.0psi @14:24:18.08 SS.reconfigure :inhaling 0.38ml/s @14:24:18.13 SS.seek 25ml @14:25:26.76 Stroke completed -- waiting for pressure to equalize ... @14:25:26.77 SS.reconfigure :exhale @14:25:26.82 SS.hold @14:26:03.60 Intake.select :close @14:26:03.75 SSV.dial :exhaust @14:26:04.51 Exhaust.select :open @14:26:04.63 SS.seek 0ml @14:26:13.43 Exhaust.select :close @14:26:13.54 SSV.dial CBV @14:26:13.57
CBV.dial SSV @14:26:14.34 Allowing 130s for 24.9ml of remaining 24.9ml @14:26:14.35 Intake.select :open @14:26:16.52 Intake @16.1psia, vacuum from 10.0 to 13.0psi @14:26:16.54 SS.reconfigure :inhaling 0.38ml/s @14:26:16.58 SS.seek 24.95ml @14:27:14.66 Vacuum too high -- waiting up to 40 seconds @14:27:14.67 SS.reconfigure :exhale @14:27:14.72 SS.hold @14:27:33.70 Intake @17.1psia, vacuum from 10.0 to 13.0psi @14:27:33.72 SS.reconfigure :inhaling 0.31ml/s @14:27:33.76 SS.seek 24.95ml @14:27:47.99 Stroke completed -- waiting for pressure to equalize ... @14:27:48.00 SS.reconfigure :exhale @14:27:48.05 SS.hold @14:28:10.57 Intake.select :close @14:28:10.72 SSV.dial :exhaust @14:28:11.48 Exhaust.select :open @14:28:11.59 SS.seek 0ml @14:28:20.41 Exhaust.select :close @14:28:20.52 Sampled 1000.0ml @14:28:20.54 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @14:28:20.55 Intake.select :close @14:28:20.61 CSV.dial :waste @14:28:20.75 SSV.dial CBV,avoiding: :exhaust @14:28:20.76
CBV.dial SSV @14:28:20.84 CS.seek 0ml @14:28:20.91 CSV.dial :air @14:28:21.78 CS.seek 10ml @14:28:22.67
CTV.dial :air @14:28:23.43 delay 15 seconds @14:28:38.45 SSV.dial :spit,avoiding: :exhaust @14:28:39.79 CTV.dial CSV,avoiding: :intake @14:28:39.85
SS.seek 0ml @14:28:39.94 SSV.dial CBV,avoiding: :exhaust @14:28:46.45 SSV.dial between CBV and plug @14:28:46.95 CBV.dial between SSV and waste2 @14:28:47.33 CSV.dial between air and CTV @14:28:47.67 CS.seek 8.452ml @14:28:52.05 CTV.dial CSV,avoiding: :air @14:28:52.06
CSV.dial CTV @14:28:52.47 CS.reconfigure :pressurize @14:28:52.52 CS.seek 0ml @14:29:07.41 BubblePoint: Intake @24.1psia, Outlet @1.1psia @14:29:07.42 CBV.dial SSV @14:29:07.76 SS.reconfigure :default @14:29:07.81 SS.seek 10ml @14:29:13.40 delay 15 seconds @14:29:28.53 Evacuated: Intake @23.6psia, Outlet @23.4psia @14:29:28.54 SSV.dial :spit,avoiding: :exhaust @14:29:29.58 SS.reconfigure :exhale @14:29:29.63 SS.seek 0ml @14:29:34.83 SS.reconfigure :fast @14:29:34.88 SS.seek 5ml @14:29:37.58 SSV.dial CBV,avoiding: :exhaust @14:29:37.60
CBV.dial SSV @14:29:38.93 CTV.dial :air,avoiding: :intake @14:29:40.24 CSV.dial CTV @14:29:40.30
SS.seek 10ml @14:29:41.45 CSV.dial :air @14:29:41.89 CS.reconfigure :fast @14:29:41.93 CS.seek 0ml @14:29:42.87
SSV.dial :spit,avoiding: :exhaust @14:29:44.19 SS.reconfigure :exhale @14:29:44.23 SS.seek 0ml @14:29:49.35 SS.reconfigure :fast @14:29:49.39 SS.seek 5ml @14:29:49.66 CS.stop @14:29:52.18 SSV.dial CBV,avoiding: :exhaust @14:29:52.21
CBV.dial SSV @14:29:54.55 SSV.dial :spit,avoiding: :exhaust @14:29:55.87 SS.reconfigure :default @14:29:55.92 SS.seek 0ml @14:29:59.18 SS.stop @14:29:59.22 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @14:29:59.23 Intake.select :close @14:29:59.30 CSV.dial :waste @14:29:59.43 SSV.dial CBV,avoiding: :exhaust @14:29:59.45
CBV.dial SSV @14:30:00.09 CS.seek 0ml @14:30:00.16 CSV.dial :air @14:30:00.79
CTV.dial :air @14:30:00.90 CS.seek 10ml @14:30:00.97
delay 15 seconds @14:30:15.99 SSV.dial :spit,avoiding: :exhaust @14:30:17.31 CTV.dial CSV,avoiding: :intake @14:30:17.35
SS.reconfigure :exhale @14:30:17.44 SS.seek 0ml @14:30:17.87 SSV.dial CBV,avoiding: :exhaust @14:30:25.48 SSV.dial between CBV and plug @14:30:25.98 CBV.dial between SSV and waste2 @14:30:26.85 CSV.dial between air and CTV @14:30:27.17 CS.seek 7.584ml @14:30:33.45 CTV.dial CSV,avoiding: :air @14:30:33.47
CSV.dial CTV @14:30:33.87 CS.reconfigure :pressurize @14:30:33.92 CS.seek 0ml @14:30:47.46 BubblePoint: Intake @24.2psia, Outlet @3.5psia @14:30:47.47 CBV.dial SSV @14:30:47.81 SS.reconfigure :default @14:30:47.86 SS.seek 10ml @14:30:53.46 delay 15 seconds @14:31:08.59 Evacuated: Intake @23.9psia, Outlet @23.8psia @14:31:08.60 SSV.dial :spit,avoiding: :exhaust @14:31:09.65 SS.reconfigure :exhale @14:31:09.70 SS.seek 0ml @14:31:14.73 SS.reconfigure :fast @14:31:14.78 SS.seek 5ml @14:31:17.54 SSV.dial CBV,avoiding: :exhaust @14:31:17.56
CBV.dial SSV @14:31:18.95 CTV.dial :air,avoiding: :intake @14:31:20.24 CSV.dial CTV @14:31:20.27
SS.seek 10ml @14:31:21.48 CSV.dial :air @14:31:21.93 CS.reconfigure :fast @14:31:21.98 CS.seek 0ml @14:31:23.04
SSV.dial :spit,avoiding: :exhaust @14:31:24.37 SS.reconfigure :exhale @14:31:24.42 SS.seek 0ml @14:31:28.56 CS.stop @14:31:29.44
SS.reconfigure :fast @14:31:29.49 SS.seek 5ml @14:31:32.31 SSV.dial CBV,avoiding: :exhaust @14:31:32.33
CBV.dial SSV @14:31:34.68 SSV.dial :spit,avoiding: :exhaust @14:31:35.99 SS.reconfigure :default @14:31:36.04 SS.seek 0ml @14:31:39.27 SS.stop @14:31:39.31 Lysing: Adding 2.0ml AqMeOH @14:31:39.32 CBV.dial between CSV and waste1,avoiding: :DBV @14:31:40.83 CTV.dial CSV @14:31:42.14 CSV.dial CSR @14:31:42.94 Pulling 1ml of CSR.AqMeOH CSR.select :AqMeOH @14:31:43.06 CS.reconfigure :default @14:31:43.10 CS.seek 1ml @14:31:47.12 delay 5 seconds @14:31:52.20 CSR.select :air @14:31:52.37 CS.seek 6.001ml @14:32:06.04 delay 2 seconds @14:32:08.05 CSV.dial :waste2 @14:32:08.51 CS.reconfigure :fast @14:32:08.56 CS.seek 0ml @14:32:23.03 CSV.dial CSR @14:32:23.61 Pulling 2ml of CSR.AqMeOH @14:32:23.62 CSR.select :AqMeOH @14:32:23.74 CS.reconfigure :default @14:32:23.79 CS.seek 2ml @14:32:29.78 delay 5 seconds @14:32:34.80 CSR.select :air @14:32:34.97 CS.seek 5.001ml @14:32:43.66 delay 2 seconds @14:32:45.68 CSV.dial CTV @14:32:46.15 CS.seek 1ml @14:32:57.49 Before lysing Collection Puck Heater at 13.1C/31.0psia @14:32:57.51 Heating Puck.from 4, :sh1 in Collection Clamp to 70C for 10:00 @14:32:57.52 CH.seek 70C,8:00 @14:35:09.22 CH.hold 10:00,70C @14:35:09.42 Begun lysing Collection Puck Heater at 68.1C/38.2psia @14:45:09.43 Ended lysing Collection Puck Heater at 70C/37.2psia @14:45:09.45 CS.seek 0ml @14:45:13.12 CTV.dial between spare and air,avoiding: :intake @14:45:14.15 CSV.dial :air @14:45:14.60 CS.seek 5ml @14:45:28.21 CSV.dial CTV @14:45:28.66 delay 2 seconds @14:45:30.68 CSV.dial :air @14:45:31.13 delay 2 seconds @14:45:33.15 CSV.dial CTV @14:45:33.61 delay 2 seconds @14:45:35.63 CSV.dial :air @14:45:36.07 delay 2 seconds @14:45:38.09 CS.seek 0ml @14:45:51.70 Puck @82.8psia after final lysis pressurization @14:45:51.72 Recovering Lysate into Collection syringe @14:45:51.73 CSV.dial CBV @14:45:52.74 CBV.dial CSV @14:45:53.13 CS.seek 5ml @14:46:06.71 delay 5 seconds @14:46:11.73 CTV.dial :air @14:46:12.68 CS.seek 7.001ml @14:46:18.62 delay 5 seconds @14:46:23.64 CSV.dial :air2 @14:46:24.11 CS.seek 10ml @14:46:32.68 CSV.dial between air and CTV,avoiding: CBV @14:46:33.84 Lysing Complete @14:46:33.91 Storing sh1 puck in Collection Clamp to tube 3 @14:46:34.09 SC.reconfigure :tight @14:46:34.14 SC.seek 3 @14:46:37.76 SC.configure :limp @14:46:37.98 Elbow.reconfigure :tight @14:46:38.03 Elbow.seek 3 @14:46:39.78 Forearm.reconfigure :tight @14:46:39.83 Forearm.seek 3 + 450 counts @14:46:40.88 SE.reconfigure :pick @14:46:40.93 SE.seek :beamBreak @14:46:42.08 SE.configure :default @14:46:42.24 Elbow.reconfigure :limp @14:46:42.29 Forearm.reconfigure :loose @14:46:42.34 Forearm.seek 3 @14:46:42.63 Elbow.hold @14:46:42.69 Elbow.reconfigure :tight @14:46:42.83 SE.seek 29.12 Pucks below Top Plate @14:46:43.27 Forearm.force -20 @14:46:43.31 Forearm.seek 3 + 286 counts @14:46:43.70 Forearm.reconfigure :tight @14:46:43.75 Forearm.seek 3 + 450 counts @14:46:43.99 SE.seek 30.12 Pucks below Top Plate @14:46:44.54 Forearm.reconfigure :loose @14:46:44.59 Forearm.seek :retract @14:46:45.54 Elbow.seek CC @14:46:47.22 Elbow.reconfigure :loose @14:46:47.75 Forearm.seek CC @14:46:48.75 Hand.close @14:46:49.43 CC.seek :open @14:46:54.17 Forearm.seek :retract @14:46:55.18 Elbow.reconfigure :tight @14:46:55.23 Elbow.seek 3 @14:46:57.00 Elbow.reconfigure :limp @14:46:57.05 Forearm.seek 3 @14:46:58.02 Elbow.hold @14:46:58.09 Elbow.reconfigure :tight @14:46:58.13 Hand.open @14:46:58.93 Forearm.force -20 @14:46:58.98 Forearm.seek 3 + 312 counts @14:46:59.28 Forearm.reconfigure :tight @14:46:59.33 Forearm.seek 3 + 450 counts @14:46:59.59 SE.seek :down @14:47:00.22 SE.stop @14:47:00.72
Forearm.reconfigure :loose @14:47:00.77 Forearm.seek :retract @14:47:01.71 Tranferring DA @14:47:01.72 Beginning samplePassC @14:47:01.73 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:47:01.93 Elbow.seek FlushPuck::Garage @14:47:03.79 Elbow.reconfigure :loose @14:47:03.90 Forearm.seek :garage @14:47:04.86 Hand.close @14:47:05.55 Forearm.seek :retract @14:47:06.58 Elbow.reconfigure :tight @14:47:07.07 Elbow.seek CC @14:47:10.03 Elbow.reconfigure :loose @14:47:10.13 Forearm.reconfigure :tight @14:47:10.18 Forearm.seek CC @14:47:11.44 CC.seek :closed @14:47:15.97 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.41 @14:47:16.03 Hand.open @14:47:16.74 Forearm.reconfigure :loose @14:47:16.78 Forearm.seek :retract @14:47:17.75 PSV.dial PSR @14:47:19.28 Pulling 1.5ml of PSR.flush @14:47:19.29 PSR.select :flush @14:47:19.42 PS.seek 1.5ml @14:47:35.62 delay 5 seconds @14:47:40.64 PSV.dial :waste1 @14:47:41.14 PS.seek 0ml @14:47:57.27 PSV.dial PSR @14:47:57.74 PSR.select :air @14:47:57.86 PS.seek 2.5ml @14:48:24.28 PRV.dial CBV,avoiding: :ab2 @14:48:24.30
CBV.dial PRV @14:48:26.04 PSV.dial :mixloop @14:48:27.07 CTV.dial :air,avoiding: :intake @14:48:27.15 PTV.dial :PRVmixing @14:48:28.48 PS.seek 0ml @14:48:54.83 PSV.dial :air @14:48:55.31 PS.seek 2.5ml @14:49:21.71 PSV.dial :mixloop @14:49:22.63 PS.seek 0ml @14:49:48.99 PSV.dial :air @14:49:49.47 PS.seek 2.5ml @14:50:15.77 PSV.dial :mixloop @14:50:16.25 PS.seek 0ml @14:50:42.81 Garaging FlushPuck in Collection Clamp @14:50:42.88 Elbow.reconfigure :tight @14:50:42.93 Elbow.seek CC @14:50:43.23 Elbow.reconfigure :loose @14:50:43.33 Forearm.seek CC @14:50:44.37 Hand.close @14:50:44.93 CC.seek :open @14:50:49.57 Forearm.seek :retract @14:50:50.54 Elbow.reconfigure :tight @14:50:50.58 Elbow.seek :garage @14:50:53.53 Elbow.reconfigure :loose @14:50:53.63 Forearm.reconfigure :tight @14:50:53.68 Forearm.seek :garage @14:50:54.69 Hand.open @14:50:55.36 Forearm.reconfigure :loose @14:50:55.41 Forearm.seek :retract @14:50:56.39 PSV.dial :mixloop @14:50:56.46 PTV.dial :PRVmixing @14:50:56.47 PRV.dial CBV @14:50:56.50
CBV.dial PRV @14:50:56.58 CTV.dial :air,avoiding: :intake @14:50:56.66 Loading lyfil puck from tube 4 into Collection Clamp @14:50:56.90 SC.reconfigure :tight @14:50:56.95 SC.seek 4 @14:51:00.53 SC.configure :limp @14:51:00.74 Elbow.reconfigure :tight @14:51:00.79 Elbow.seek 4 @14:51:03.12 Forearm.reconfigure :tight @14:51:03.20 Forearm.seek 4 + 450 counts @14:51:04.25 SE.reconfigure :pick @14:51:04.31 SE.seek :beamBreak @14:51:07.49 SE.configure :default @14:51:07.65 Elbow.seek 4 @14:51:07.83 Forearm.seek 4 + 300 counts @14:51:07.97 Forearm.reconfigure :loose @14:51:08.02 Hand.close @14:51:08.69 SE.seek :down @14:51:08.89
Forearm.seek :retract @14:51:09.86 Elbow.seek CC @14:51:11.39 SE.stop @14:51:11.51
Elbow.reconfigure :loose @14:51:11.61 Forearm.reconfigure :tight @14:51:11.66 Forearm.seek CC @14:51:12.94 CC.seek :closed @14:51:17.46 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.18 @14:51:17.47 Hand.open @14:51:18.11 Forearm.reconfigure :loose @14:51:18.15 Forearm.seek :retract @14:51:19.14 CTV.dial CSV,avoiding: :intake @14:51:20.49 CSV.dial CTV @14:51:20.82 PS.seek 2.5ml @14:51:47.77 CS.seek 4.5ml @14:52:02.70 delay 8 seconds @14:52:10.73 PSV.dial :air @14:52:11.19 delay 2 seconds @14:52:13.21 PSV.dial :waste2 @14:52:13.95 PS.seek 0ml @14:52:40.36 CBV.dial between PRV and waste1 @14:52:40.69 CSV.dial :air @14:52:41.16 CS.seek 10ml @14:52:56.05 CSV.dial CTV @14:52:56.51 CS.seek 0ml @14:53:22.89 CBV.dial :waste1 @14:53:23.21 delay 5 seconds @14:53:28.23 CTV.dial :air,avoiding: :intake @14:53:29.64 Storing lyfil puck in Collection Clamp to tube 3 @14:53:29.82 SC.reconfigure :tight @14:53:29.86 SC.seek 3 @14:53:33.52 SC.configure :limp @14:53:33.73 Elbow.reconfigure :tight @14:53:33.78 Elbow.seek 3 @14:53:35.57 Forearm.reconfigure :tight @14:53:35.62 Forearm.seek 3 + 450 counts @14:53:36.62 SE.reconfigure :pick @14:53:36.68 SE.seek :beamBreak @14:53:37.75 SE.configure :default @14:53:37.91 Elbow.reconfigure :limp @14:53:37.96 Forearm.reconfigure :loose @14:53:38.01 Forearm.seek 3 @14:53:38.28 Elbow.hold @14:53:38.35 Elbow.reconfigure :tight @14:53:38.45 SE.seek 30.11 Pucks below Top Plate @14:53:38.93 Forearm.force -20 @14:53:39.34 Forearm.seek 3 + 318 counts @14:53:39.82 Forearm.reconfigure :tight @14:53:39.87 Forearm.seek 3 + 450 counts @14:53:40.09 SE.seek 31.11 Pucks below Top Plate @14:53:40.66 Forearm.reconfigure :loose @14:53:40.71 Forearm.seek :retract @14:53:41.64 Elbow.seek CC @14:53:43.31 Elbow.reconfigure :loose @14:53:43.41 Forearm.seek CC @14:53:44.36 Hand.close @14:53:44.92 CC.seek :open @14:53:49.76 Forearm.seek :retract @14:53:50.75 Elbow.reconfigure :tight @14:53:50.80 Elbow.seek 3 @14:53:52.47 Elbow.reconfigure :limp @14:53:52.53 Forearm.seek 3 @14:53:53.59 Elbow.hold @14:53:53.66 Elbow.reconfigure :tight @14:53:53.71 Hand.open @14:53:54.42 Forearm.force -20 @14:53:54.47 Forearm.seek 3 + 302 counts @14:53:54.87 Forearm.reconfigure :tight @14:53:54.92 Forearm.seek 3 + 450 counts @14:53:55.18 SE.seek :down @14:53:55.84 SE.stop @14:53:56.68
Forearm.reconfigure :loose @14:53:56.79 Forearm.seek :retract @14:53:57.73 PSV.dial :air @14:53:58.49 PS.seek 2.5ml @14:54:24.80 delay 1 second @14:54:25.82 PSV.dial :mixloop @14:54:26.27 delay 1 second @14:54:27.29 PSV.dial :air @14:54:27.89 delay 1 second @14:54:28.91 PSV.dial :mixloop @14:54:29.35 delay 1 second @14:54:30.36 PSV.dial :air @14:54:30.83 delay 1 second @14:54:31.85 PS.seek 0ml @14:54:58.14 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @14:54:58.34 Elbow.seek FlushPuck::Garage @14:55:00.20 Elbow.reconfigure :loose @14:55:00.30 Forearm.seek :garage @14:55:01.29 Hand.close @14:55:02.04 Forearm.seek :retract @14:55:03.06 Elbow.reconfigure :tight @14:55:03.11 Elbow.seek PC @14:55:04.38 Elbow.reconfigure :loose @14:55:04.48 Forearm.reconfigure :tight @14:55:04.53 Forearm.seek PC @14:55:05.83 PC.seek :closed @14:55:10.46 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.63 @14:55:10.53 Hand.open @14:55:11.61 Forearm.reconfigure :loose @14:55:11.72 Forearm.seek :retract @14:55:12.67 PRV.dial :air @14:55:13.15 PSV.dial :mixloop @14:55:13.69 PS.seek 0.3ml @14:55:17.61 PSV.dial PSR @14:55:18.78 Pulling 0.4ml of PSR.air @14:55:18.79 PSR.select :air @14:55:18.82 PS.seek 0.7ml @14:55:23.67 delay 3 seconds @14:55:26.69 PSR.select :air @14:55:26.71 PS.seek 2.5ml @14:55:45.95 delay 3 seconds @14:55:48.97 PSV.dial :mixloop @14:55:50.00 PTV.dial :mixing @14:55:50.75 PBV.dial :waste2 @14:55:51.73 PS.seek 0ml @14:56:18.14 Garaging FlushPuck in Processing Clamp @14:56:18.22 Elbow.reconfigure :tight @14:56:18.26 Elbow.seek PC @14:56:18.39 Elbow.reconfigure :loose @14:56:18.49 Forearm.seek PC @14:56:19.42 Hand.close @14:56:20.05 PC.seek :open @14:56:24.76 Forearm.seek :retract @14:56:25.80 Elbow.reconfigure :tight @14:56:25.84 Elbow.seek :garage @14:56:27.21 Elbow.reconfigure :loose @14:56:27.31 Forearm.reconfigure :tight @14:56:27.36 Forearm.seek :garage @14:56:28.35 Hand.open @14:56:28.98 Forearm.reconfigure :loose @14:56:29.03 Forearm.seek :retract @14:56:30.02 Loading da puck from tube 4 into Processing Clamp @14:56:30.25 SC.reconfigure :tight @14:56:30.30 SC.seek 4 @14:56:33.88 SC.configure :limp @14:56:34.53 Elbow.reconfigure :tight @14:56:34.58 Elbow.seek 4 @14:56:36.44 Forearm.reconfigure :tight @14:56:36.48 Forearm.seek 4 + 450 counts @14:56:37.51 SE.reconfigure :pick @14:56:37.57 SE.seek :beamBreak @14:56:40.64 SE.configure :default @14:56:40.80 Elbow.seek 4 @14:56:41.14 Forearm.seek 4 + 300 counts @14:56:41.43 Forearm.reconfigure :loose @14:56:41.47 Hand.close @14:56:42.06 SE.seek :down @14:56:42.26
Forearm.seek :retract @14:56:43.21 Elbow.seek PC @14:56:44.87 SE.stop @14:56:45.73
Elbow.reconfigure :loose @14:56:45.83 Forearm.reconfigure :tight @14:56:45.88 Forearm.seek PC @14:56:47.14 PC.seek :closed @14:56:51.78 Processing Clamp onSpring/offSpring for 16/26 samples, on/off torque 1.65 @14:56:51.79 Hand.open @14:56:52.47 Forearm.reconfigure :loose @14:56:52.52 Forearm.seek :retract @14:56:53.47 Heating Puck.from 4, :da in Processing Clamp to 28C for 2:00:00 @14:56:53.49 PH.seek 28C,5:00 @14:57:35.84 PH.hold 2:00:00,28C @14:57:35.89 delay 1:30 @14:59:05.94 PSR.select :air @14:59:05.96 PSV.dial PSR @14:59:07.01 Pulling 0.5ml of PSR.wash @14:59:07.02 PSR.select :wash @14:59:07.14 PS.seek 0.5ml @14:59:13.04 delay 5 seconds @14:59:18.06 PSR.select :air @14:59:18.19 PS.seek 2ml @14:59:34.29 PTV.dial :puck @14:59:35.04 PSV.dial PTV @14:59:35.81 PBV.dial :waste1 @14:59:37.20 PS.seek 1.5ml @14:59:43.17 delay 3 seconds @14:59:46.19 PBV.dial between PSV and waste1 @14:59:46.97 PS.seek 0ml @15:00:03.08 delay 5:00 @15:05:03.09 recoverPSRreagent08 @15:05:03.10 PTV.dial :puck @15:05:03.18 PBV.dial :waste1 @15:05:03.54 delay 30 seconds @15:05:33.56 PBV.dial between PSV and waste1 @15:05:33.93 PSR.select :air @15:05:33.95 PSV.dial PSR @15:05:34.72 PS.reconfigure :fast @15:05:34.76 PS.seek 2.5ml @15:05:58.60 PSV.dial PTV @15:05:59.37 delay 5 seconds @15:06:04.39 PSV.dial PSR @15:06:05.13 delay 5 seconds @15:06:10.15 PSV.dial PTV @15:06:10.91 delay 5 seconds @15:06:15.93 PSV.dial :air @15:06:16.81 PS.seek 0ml @15:06:39.94 PTV.dial :PRVmixing,via: PRV @15:06:41.27 PSV.dial :mixloop @15:06:41.72 PRV.dial :ab1 @15:06:42.48 PS.reconfigure :slow @15:06:42.53 PS.seek 0.15ml @15:06:46.38 delay 2 seconds @15:06:48.40 CBV.dial PRV @15:06:48.42
PRV.dial CBV @15:06:49.00 PS.seek 0.35ml @15:06:53.66 delay 2 seconds @15:06:55.68 CBV.dial between PRV and PSV @15:06:56.00 PRV.dial :air @15:06:56.46 PS.seek 0.9ml @15:07:08.28 PRV.dial :waste2 @15:07:09.04 PS.seek 0.5ml @15:07:17.69 addPMLReagent08 @15:07:17.70 PSV.dial :air @15:07:18.18 PS.reconfigure :default @15:07:18.23 PS.seek 2ml @15:07:34.41 PSV.dial :PML @15:07:34.88 PTV.dial :mixing @15:07:35.63 PBV.dial :waste1 @15:07:36.07 PS.seek 1.85ml @15:07:38.41 PBV.dial between PSV and waste1 @15:07:38.79 PS.seek 0ml @15:07:58.53 delay 2 seconds @15:08:00.56 Beginning samplePassC @15:08:00.57 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @15:08:01.17 Elbow.reconfigure :tight @15:08:01.23 Elbow.seek FlushPuck::Garage @15:08:02.61 Elbow.reconfigure :loose @15:08:02.71 Forearm.seek :garage @15:08:03.67 Hand.close @15:08:04.41 Forearm.seek :retract @15:08:05.38 Elbow.reconfigure :tight @15:08:05.43 Elbow.seek CC @15:08:08.34 Elbow.reconfigure :loose @15:08:08.44 Forearm.reconfigure :tight @15:08:08.49 Forearm.seek CC @15:08:09.78 CC.seek :closed @15:08:14.29 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.43 @15:08:14.36 Hand.open @15:08:14.97 Forearm.reconfigure :loose @15:08:15.02 Forearm.seek :retract @15:08:15.99 PSV.dial PSR @15:08:17.05 Pulling 1.5ml of PSR.flush @15:08:17.06 PSR.select :flush @15:08:17.18 PS.seek 1.5ml @15:08:33.31 delay 5 seconds @15:08:38.33 PSV.dial :waste1 @15:08:38.79 PS.seek 0ml @15:08:54.97 PSV.dial PSR @15:08:55.44 PSR.select :air @15:08:55.56 PS.seek 2.5ml @15:09:21.95 PRV.dial CBV,avoiding: :ab2 @15:09:21.97
CBV.dial PRV @15:09:23.60 PSV.dial :mixloop @15:09:24.65 CTV.dial :air,avoiding: :intake @15:09:24.72 PTV.dial :PRVmixing @15:09:25.49 PS.seek 0ml @15:09:51.91 PSV.dial :air @15:09:52.38 PS.seek 2.5ml @15:10:18.78 PSV.dial :mixloop @15:10:19.38 PS.seek 0ml @15:10:45.69 PSV.dial :air @15:10:46.15 PS.seek 2.5ml @15:11:12.47 PSV.dial :mixloop @15:11:12.94 PS.seek 0ml @15:11:39.28 Garaging FlushPuck in Collection Clamp @15:11:39.35 Elbow.reconfigure :tight @15:11:39.40 Elbow.seek CC @15:11:39.75 Elbow.reconfigure :loose @15:11:39.85 Forearm.seek CC @15:11:40.86 Hand.close @15:11:41.54 CC.seek :open @15:11:46.15 Forearm.seek :retract @15:11:47.10 Elbow.reconfigure :tight @15:11:47.14 Elbow.seek :garage @15:11:49.95 Elbow.reconfigure :loose @15:11:50.05 Forearm.reconfigure :tight @15:11:50.10 Forearm.seek :garage @15:11:51.01 Hand.open @15:11:51.72 Forearm.reconfigure :loose @15:11:51.77 Forearm.seek :retract @15:11:52.74 delayUntil "15:16:00.54" @15:11:52.77 Whole Cell Archive sampling 1000ml @15:11:52.78 Loading wcr puck from tube 5 into Collection Clamp @15:11:53.02 SC.reconfigure :tight @15:11:53.07 SC.seek 5 @15:11:56.55 SC.configure :limp @15:11:57.22 Elbow.reconfigure :tight @15:11:57.27 Elbow.seek 5 @15:11:59.19 Forearm.reconfigure :tight @15:11:59.24 Forearm.seek 5 + 450 counts @15:12:00.27 SE.reconfigure :pick @15:12:00.33 SE.seek :beamBreak @15:12:01.27 SE.configure :default @15:12:01.44 Elbow.seek 5 @15:12:01.63 Forearm.seek 5 + 300 counts @15:12:01.86 Forearm.reconfigure :loose @15:12:01.91 Hand.close @15:12:02.55 SE.seek :down @15:12:02.76 Forearm.seek :retract @15:12:03.12 SE.stop @15:12:03.71 Elbow.seek CC @15:12:05.28 Elbow.reconfigure :loose @15:12:05.39 Forearm.reconfigure :tight @15:12:05.44 Forearm.seek CC @15:12:06.68 CC.seek :closed @15:12:11.78 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.26 @15:12:11.79 Hand.open @15:12:12.47 Forearm.reconfigure :loose @15:12:12.53 Forearm.seek :retract @15:12:13.52 collectSample 1000ml w/bubble point of 35psi @15:12:13.53 Priming 7ml @15:12:13.54 Intake.select :close @15:12:13.56 Exhaust.select :close @15:12:13.57 SS.hold @15:12:13.79 Calibrating pressure sensors @15:12:13.80 CTV.dial :air @15:12:13.88 SSV.dial :spit,avoiding: :exhaust @15:12:13.97 SS.reconfigure :fast @15:12:14.02 SS.seek 5ml @15:12:16.66 SSV.dial CBV @15:12:16.68 CBV.dial SSV @15:12:18.06 delay 3.8 seconds @15:12:21.99 Sensor limits: Intake max 103.7psia; Outlet max 95.4psia @15:12:22.00 SSV.dial :spit @15:12:23.33 SS.seek 0ml @15:12:26.12 SSV.dial CBV @15:12:27.45 CTV.dial :intake @15:12:28.22 SSV.dial CBV @15:12:28.24 CBV.dial SSV @15:12:28.39 Allowing 40s for 7.0ml of remaining 7.0ml @15:12:28.41 Intake.select :open @15:12:30.57 Intake @14.3psia, vacuum from 8.8 to 11.8psi @15:12:30.59 SS.reconfigure :inhaling 2.20ml/s @15:12:30.64 SS.seek 7ml @15:12:37.32 Stroke completed -- waiting for pressure to equalize ... @15:12:37.33 SS.reconfigure :exhale @15:12:37.38 SS.hold @15:12:52.22 Intake.select :close @15:12:52.38 SSV.dial :exhaust @15:12:53.16 Exhaust.select :open @15:12:53.28 SS.seek 0ml @15:12:57.60 Exhaust.select :close @15:12:57.71 Sampling 1000.0ml @15:12:57.73 Intake.select :close @15:12:57.74 Exhaust.select :close @15:12:57.75 SS.reconfigure :default @15:12:57.80 SS.hold @15:12:58.00 CTV.dial :intake @15:12:58.18 SSV.dial CBV @15:12:58.20 CBV.dial SSV @15:12:58.98 Allowing 130s for 25.0ml of remaining 1000.0ml @15:12:58.99 Intake.select :open @15:13:01.16 Intake @15.4psia, vacuum from 9.9 to 12.9psi @15:13:01.18 SS.reconfigure :inhaling 2.20ml/s @15:13:01.23 SS.seek 25ml @15:13:09.42 Vacuum too high -- waiting up to 40 seconds @15:13:09.43 SS.reconfigure :exhale @15:13:09.49 SS.hold @15:13:18.65 Intake @17.1psia, vacuum from 10.0 to 13.0psi @15:13:18.67 SS.reconfigure :inhaling 1.76ml/s @15:13:18.72 SS.seek 25ml @15:13:22.57 Vacuum too high -- waiting up to 40 seconds @15:13:22.59 SS.reconfigure :exhale @15:13:22.65 SS.hold @15:13:26.48 Intake @17.2psia, vacuum from 10.0 to 13.0psi @15:13:26.50 SS.reconfigure :inhaling 1.41ml/s @15:13:26.54 SS.seek 25ml @15:13:31.11 Vacuum too high -- waiting up to 40 seconds @15:13:31.12 SS.reconfigure :exhale @15:13:31.17 SS.hold @15:13:35.20 Intake @17.8psia, vacuum from 10.0 to 13.0psi @15:13:35.22 SS.reconfigure :inhaling 1.13ml/s @15:13:35.27 SS.seek 25ml @15:13:37.00 Stroke completed -- waiting for pressure to equalize ... @15:13:37.02 SS.reconfigure :exhale @15:13:37.07 SS.hold @15:14:05.78 Intake.select :close @15:14:05.93 SSV.dial :exhaust @15:14:06.69 Exhaust.select :open @15:14:06.81 SS.seek 0ml @15:14:15.88 Exhaust.select :close @15:14:15.99 SSV.dial CBV @15:14:16.01 CBV.dial SSV @15:14:16.79 Allowing 130s for 25.0ml of remaining 975.0ml @15:14:16.80 Intake.select :open @15:14:18.97 Intake @15.9psia, vacuum from 10.0 to 13.0psi @15:14:18.99 SS.reconfigure :inhaling 1.13ml/s @15:14:19.04 SS.seek 25ml @15:14:38.83 Vacuum too high -- waiting up to 40 seconds @15:14:38.85 SS.reconfigure :exhale @15:14:38.90 SS.hold @15:14:47.62 Intake @19.1psia, vacuum from 10.0 to 13.0psi @15:14:47.64 SS.reconfigure :inhaling 0.91ml/s @15:14:47.69 SS.seek 25ml @15:14:51.92 Vacuum too high -- waiting up to 40 seconds @15:14:51.93 SS.reconfigure :exhale @15:14:51.99 SS.hold @15:14:53.34 Intake @17.5psia, vacuum from 10.0 to 13.0psi @15:14:53.36 SS.reconfigure :inhaling 0.73ml/s @15:14:53.40 SS.seek 25ml @15:14:56.05 Stroke completed -- waiting for pressure to equalize ... @15:14:56.06 SS.reconfigure :exhale @15:14:56.11 SS.hold @15:15:24.76 Intake.select :close @15:15:24.92 SSV.dial :exhaust @15:15:25.71 Exhaust.select :open @15:15:25.83 SS.seek 0ml @15:15:34.61 Exhaust.select :close @15:15:34.72 SSV.dial CBV @15:15:34.76 CBV.dial SSV @15:15:35.54 Allowing 130s for 25.0ml of remaining 950.0ml @15:15:35.55 Intake.select :open @15:15:37.72 Intake @15.9psia, vacuum from 10.0 to 13.0psi @15:15:37.74 SS.reconfigure :inhaling 0.73ml/s @15:15:37.79 SS.seek 25ml @15:16:00.56 recoverPRVreagent08 @15:16:00.57 PTV.dial :mixing @15:16:01.34 PBV.dial :waste1 @15:16:01.71 delay 30 seconds @15:16:14.28 Stroke completed -- waiting for pressure to equalize ... @15:16:14.29 SS.reconfigure :exhale @15:16:14.34 SS.hold @15:16:31.73 PBV.dial between PSV and waste1 @15:16:32.10 PSV.dial :air @15:16:32.70 PS.reconfigure :fast @15:16:32.75 PS.seek 2.5ml @15:16:43.41 Intake.select :close @15:16:43.56 SSV.dial :exhaust @15:16:44.40 Exhaust.select :open @15:16:44.52 SS.seek 0ml @15:16:53.55 Exhaust.select :close @15:16:53.66 SSV.dial CBV @15:16:53.68 CBV.dial SSV @15:16:54.49 Allowing 130s for 25.0ml of remaining 925.0ml @15:16:54.50 Intake.select :open @15:16:56.62 PSV.dial :mixloop @15:16:56.72 Intake @16.0psia, vacuum from 10.0 to 13.0psi @15:16:56.74 SS.reconfigure :inhaling 0.73ml/s @15:16:56.79 SS.seek 25ml @15:16:57.07 delay 2 seconds @15:16:59.09 PSV.dial :air @15:16:59.56 delay 2 seconds @15:17:01.57 PSV.dial :mixloop @15:17:02.04 delay 2 seconds @15:17:04.06 PSV.dial :air @15:17:04.54 PS.seek 0ml @15:17:27.96 washCycleDA #1 of 2 @15:17:27.97 PSR.select :air @15:17:27.99 PSV.dial PSR @15:17:29.34 Pulling 0.5ml of PSR.wash @15:17:29.35 PSR.select :wash @15:17:29.47 PS.reconfigure :default @15:17:29.52 PS.seek 0.5ml @15:17:32.73 Stroke completed -- waiting for pressure to equalize ... @15:17:32.75 SS.reconfigure :exhale @15:17:32.79 SS.hold @15:17:35.52 delay 5 seconds @15:17:40.54 PSR.select :air @15:17:41.10 PS.seek 2ml @15:17:57.24 PTV.dial :puck @15:17:57.99 PSV.dial PTV @15:17:58.74 PBV.dial :waste1 @15:17:59.18 PS.seek 1.5ml @15:18:01.50 Intake.select :close @15:18:01.66 SSV.dial :exhaust @15:18:02.42 Exhaust.select :open @15:18:02.54 SS.seek 0ml @15:18:05.12 delay 3 seconds @15:18:08.14 PBV.dial between PSV and waste1 @15:18:08.51 PS.seek 0ml @15:18:11.50 Exhaust.select :close @15:18:11.61 SSV.dial CBV @15:18:11.65 CBV.dial SSV @15:18:12.41 Allowing 130s for 25.0ml of remaining 900.0ml @15:18:12.42 Intake.select :open @15:18:14.59 Intake @16.0psia, vacuum from 10.0 to 13.0psi @15:18:14.61 SS.reconfigure :inhaling 0.73ml/s @15:18:14.66 SS.seek 25ml @15:18:24.68 delay 2:00 @15:18:50.76 Stroke completed -- waiting for pressure to equalize ... @15:18:50.78 SS.reconfigure :exhale @15:18:50.83 SS.hold @15:19:13.59 Intake.select :close @15:19:13.75 SSV.dial :exhaust @15:19:14.51 Exhaust.select :open @15:19:14.63 SS.seek 0ml @15:19:23.42 Exhaust.select :close @15:19:23.53 SSV.dial CBV @15:19:23.57 CBV.dial SSV @15:19:24.33 Allowing 130s for 25.0ml of remaining 875.0ml @15:19:24.34 Intake.select :open @15:19:26.89 Intake @16.1psia, vacuum from 10.0 to 13.0psi @15:19:26.98 SS.reconfigure :inhaling 0.73ml/s @15:19:27.03 SS.seek 25ml @15:20:03.29 Stroke completed -- waiting for pressure to equalize ... @15:20:03.31 SS.reconfigure :exhale @15:20:03.35 SS.hold @15:20:24.70 recoverPSRreagent08 @15:20:24.72 PTV.dial :puck @15:20:24.90 PBV.dial :waste1 @15:20:25.27 delay 30 seconds @15:20:25.83 Intake.select :close @15:20:26.44 SSV.dial :exhaust @15:20:27.22 Exhaust.select :open @15:20:27.34 SS.seek 0ml @15:20:36.17 Exhaust.select :close @15:20:36.28 SSV.dial CBV @15:20:36.31 CBV.dial SSV @15:20:37.09 Allowing 130s for 25.0ml of remaining 850.0ml @15:20:37.11 Intake.select :open @15:20:39.27 Intake @16.0psia, vacuum from 10.0 to 13.0psi @15:20:39.29 SS.reconfigure :inhaling 0.73ml/s @15:20:39.34 SS.seek 25ml @15:20:55.29 PBV.dial between PSV and waste1 @15:20:55.66 PSR.select :air @15:20:55.68 PSV.dial PSR @15:20:56.45 PS.reconfigure :fast @15:20:56.50 PS.seek 2.5ml @15:21:15.71 Stroke completed -- waiting for pressure to equalize ... @15:21:15.73 SS.reconfigure :exhale @15:21:15.78 SS.hold @15:21:20.39 PSV.dial PTV @15:21:21.16 delay 5 seconds @15:21:26.18 PSV.dial PSR @15:21:26.95 delay 5 seconds @15:21:31.97 PSV.dial PTV @15:21:32.72 delay 5 seconds @15:21:37.74 PSV.dial :air @15:21:38.27 Intake.select :close @15:21:38.43 SSV.dial :exhaust @15:21:38.53 PS.seek 0ml @15:21:39.20 Exhaust.select :open @15:21:39.32 SS.seek 0ml @15:21:48.37 Exhaust.select :close @15:21:48.48 SSV.dial CBV @15:21:48.51 CBV.dial SSV @15:21:49.31 Allowing 130s for 25.0ml of remaining 825.0ml @15:21:49.32 Intake.select :open @15:21:51.49 Intake @16.0psia, vacuum from 10.0 to 13.0psi @15:21:51.51 SS.reconfigure :inhaling 0.73ml/s @15:21:51.56 SS.seek 25ml @15:22:02.02 washCycleDA #2 of 2 @15:22:02.03 PSR.select :air @15:22:02.05 PSV.dial PSR @15:22:03.39 Pulling 0.5ml of PSR.wash @15:22:03.41 PSR.select :wash @15:22:03.52 PS.reconfigure :default @15:22:03.57 PS.seek 0.5ml @15:22:09.55 delay 5 seconds @15:22:14.57 PSR.select :air @15:22:14.69 PS.seek 2ml @15:22:27.26 Stroke completed -- waiting for pressure to equalize ... @15:22:27.27 SS.reconfigure :exhale @15:22:27.32 SS.hold @15:22:30.83 PTV.dial :puck @15:22:30.91 PSV.dial PTV @15:22:31.78 PBV.dial :waste1 @15:22:32.22 PS.seek 1.5ml @15:22:38.20 delay 3 seconds @15:22:41.22 PBV.dial between PSV and waste1 @15:22:41.60 PS.seek 0ml @15:22:52.31 Intake.select :close @15:22:52.47 SSV.dial :exhaust @15:22:53.23 Exhaust.select :open @15:22:53.35 SS.seek 0ml @15:22:57.80 delay 2:00 @15:23:02.18 Exhaust.select :close @15:23:02.30 SSV.dial CBV @15:23:02.81 CBV.dial SSV @15:23:03.56 Allowing 130s for 25.0ml of remaining 800.0ml @15:23:03.57 Intake.select :open @15:23:05.75 Intake @15.9psia, vacuum from 10.0 to 13.0psi @15:23:05.76 SS.reconfigure :inhaling 0.73ml/s @15:23:05.81 SS.seek 25ml @15:23:42.15 Stroke completed -- waiting for pressure to equalize ... @15:23:42.16 SS.reconfigure :exhale @15:23:42.21 SS.hold @15:24:05.06 Intake.select :close @15:24:05.28 SSV.dial :exhaust @15:24:06.06 Exhaust.select :open @15:24:06.17 SS.seek 0ml @15:24:14.94 Exhaust.select :close @15:24:15.05 SSV.dial CBV @15:24:15.10 CBV.dial SSV @15:24:15.84 Allowing 130s for 25.0ml of remaining 775.0ml @15:24:15.85 Intake.select :open @15:24:18.02 Intake @16.1psia, vacuum from 10.0 to 13.0psi @15:24:18.04 SS.reconfigure :inhaling 0.73ml/s @15:24:18.09 SS.seek 25ml @15:24:47.51 Vacuum too high -- waiting up to 40 seconds @15:24:47.52 SS.reconfigure :exhale @15:24:47.58 SS.hold @15:24:55.18 Intake @16.6psia, vacuum from 10.0 to 13.0psi @15:24:55.20 SS.reconfigure :inhaling 0.59ml/s @15:24:55.24 SS.seek 25ml @15:24:57.82 recoverPSRreagent08 @15:24:57.84 PTV.dial :puck @15:24:57.92 PBV.dial :waste1 @15:24:58.30 delay 30 seconds @15:25:04.40 Stroke completed -- waiting for pressure to equalize ... @15:25:04.46 SS.reconfigure :exhale @15:25:04.50 SS.hold @15:25:19.26 Intake.select :close @15:25:19.42 SSV.dial :exhaust @15:25:20.21 Exhaust.select :open @15:25:20.33 SS.seek 0ml @15:25:28.32 PBV.dial between PSV and waste1 @15:25:28.70 PSR.select :air @15:25:28.72 PSV.dial PSR @15:25:29.11 Exhaust.select :close @15:25:29.22 SSV.dial CBV @15:25:29.26 CBV.dial SSV @15:25:29.47 PS.reconfigure :fast @15:25:29.52 PS.seek 2.5ml @15:25:30.04 Allowing 130s for 25.0ml of remaining 750.0ml @15:25:30.05 Intake.select :open @15:25:32.22 Intake @16.1psia, vacuum from 10.0 to 13.0psi @15:25:32.67 SS.reconfigure :inhaling 0.59ml/s @15:25:32.75 SS.seek 25ml @15:25:53.67 PSV.dial PTV @15:25:54.44 delay 5 seconds @15:25:59.46 PSV.dial PSR @15:26:00.22 delay 5 seconds @15:26:05.24 PSV.dial PTV @15:26:06.13 delay 5 seconds @15:26:11.15 PSV.dial :air @15:26:11.93 PS.seek 0ml @15:26:17.97 Stroke completed -- waiting for pressure to equalize ... @15:26:17.99 SS.reconfigure :exhale @15:26:18.03 SS.hold @15:26:35.20 getPMLReagent08 @15:26:35.21 PRV.dial :air @15:26:35.67 delay 5 seconds @15:26:38.47 Intake.select :close @15:26:38.63 SSV.dial :exhaust @15:26:39.42 Exhaust.select :open @15:26:39.54 SS.seek 0ml @15:26:40.69 PTV.dial :PRVmixing @15:26:42.02 delay 5 seconds @15:26:47.04 PRV.dial :ab2 @15:26:47.50 PTV.dial :PRVmixing @15:26:47.58 PSV.dial :mixloop @15:26:48.07 PS.reconfigure :default @15:26:48.11 PS.seek 0.3ml @15:26:48.30 Exhaust.select :close @15:26:48.42 SSV.dial CBV @15:26:48.44 CBV.dial SSV @15:26:49.23 Allowing 130s for 25.0ml of remaining 725.0ml @15:26:49.24 Intake.select :open @15:26:51.41 Intake @15.9psia, vacuum from 10.0 to 13.0psi @15:26:51.42 SS.reconfigure :inhaling 0.59ml/s @15:26:51.47 SS.seek 25ml @15:26:52.15 delay 2 seconds @15:26:54.16 PRV.dial :air @15:26:54.68 PS.seek 0.55ml @15:26:58.08 addPMLReagent08 @15:26:58.09 PSV.dial :air @15:26:58.57 PS.seek 2ml @15:27:14.26 PSV.dial :PML @15:27:14.73 PTV.dial :mixing @15:27:15.51 PBV.dial :waste1 @15:27:15.95 PS.seek 1.85ml @15:27:18.29 PBV.dial between PSV and waste1 @15:27:18.66 PS.seek 0ml @15:27:36.20 Stroke completed -- waiting for pressure to equalize ... @15:27:36.22 SS.reconfigure :exhale @15:27:36.27 SS.hold @15:27:38.39 delay 2 seconds @15:27:40.40 delay 8:00 @15:27:58.92 Intake.select :close @15:27:59.08 SSV.dial :exhaust @15:27:59.85 Exhaust.select :open @15:27:59.97 SS.seek 0ml @15:28:08.94 Exhaust.select :close @15:28:09.05 SSV.dial CBV @15:28:09.09 CBV.dial SSV @15:28:09.85 Allowing 130s for 25.0ml of remaining 700.0ml @15:28:09.86 Intake.select :open @15:28:12.03 Intake @15.9psia, vacuum from 10.0 to 13.0psi @15:28:12.05 SS.reconfigure :inhaling 0.59ml/s @15:28:12.10 SS.seek 25ml @15:28:56.47 Stroke completed -- waiting for pressure to equalize ... @15:28:56.48 SS.reconfigure :exhale @15:28:56.53 SS.hold @15:29:21.53 Intake.select :close @15:29:21.69 SSV.dial :exhaust @15:29:22.46 Exhaust.select :open @15:29:22.57 SS.seek 0ml @15:29:31.38 Exhaust.select :close @15:29:31.49 SSV.dial CBV @15:29:31.51 CBV.dial SSV @15:29:32.28 Allowing 130s for 25.0ml of remaining 675.0ml @15:29:32.30 Intake.select :open @15:29:34.93 Intake @15.9psia, vacuum from 10.0 to 13.0psi @15:29:34.95 SS.reconfigure :inhaling 0.59ml/s @15:29:35.00 SS.seek 25ml @15:30:13.44 Vacuum too high -- waiting up to 40 seconds @15:30:13.45 SS.reconfigure :exhale @15:30:13.51 SS.hold @15:30:26.37 Intake @17.5psia, vacuum from 10.0 to 13.0psi @15:30:26.39 SS.reconfigure :inhaling 0.47ml/s @15:30:26.43 SS.seek 25ml @15:30:34.59 Stroke completed -- waiting for pressure to equalize ... @15:30:34.60 SS.reconfigure :exhale @15:30:34.65 SS.hold @15:30:51.35 Intake.select :close @15:30:51.51 SSV.dial :exhaust @15:30:52.27 Exhaust.select :open @15:30:52.38 SS.seek 0ml @15:31:01.22 Exhaust.select :close @15:31:01.34 SSV.dial CBV @15:31:01.36 CBV.dial SSV @15:31:02.16 Allowing 130s for 25.0ml of remaining 650.0ml @15:31:02.17 Intake.select :open @15:31:04.34 Intake @15.8psia, vacuum from 10.0 to 13.0psi @15:31:04.36 SS.reconfigure :inhaling 0.47ml/s @15:31:04.41 SS.seek 25ml @15:31:59.47 Stroke completed -- waiting for pressure to equalize ... @15:31:59.48 SS.reconfigure :exhale @15:31:59.53 SS.hold @15:32:24.35 Intake.select :close @15:32:24.51 SSV.dial :exhaust @15:32:25.28 Exhaust.select :open @15:32:25.40 SS.seek 0ml @15:32:34.40 Exhaust.select :close @15:32:34.51 SSV.dial CBV @15:32:34.53 CBV.dial SSV @15:32:35.33 Allowing 130s for 25.0ml of remaining 625.0ml @15:32:35.34 Intake.select :open @15:32:37.92 Intake @15.9psia, vacuum from 10.0 to 13.0psi @15:32:37.94 SS.reconfigure :inhaling 0.47ml/s @15:32:37.99 SS.seek 25ml @15:33:33.40 Stroke completed -- waiting for pressure to equalize ... @15:33:33.41 SS.reconfigure :exhale @15:33:33.46 SS.hold @15:34:02.57 Intake.select :close @15:34:02.73 SSV.dial :exhaust @15:34:03.50 Exhaust.select :open @15:34:03.62 SS.seek 0ml @15:34:12.64 Exhaust.select :close @15:34:12.75 SSV.dial CBV @15:34:12.78 CBV.dial SSV @15:34:13.56 Allowing 130s for 25.0ml of remaining 600.0ml @15:34:13.58 Intake.select :open @15:34:15.74 Intake @15.9psia, vacuum from 10.0 to 13.0psi @15:34:15.76 SS.reconfigure :inhaling 0.47ml/s @15:34:15.81 SS.seek 25ml @15:35:11.20 Stroke completed -- waiting for pressure to equalize ... @15:35:11.21 SS.reconfigure :exhale @15:35:11.26 SS.hold @15:35:40.42 recoverPRVreagent08 @15:35:40.43 PTV.dial :mixing @15:35:40.61 PBV.dial :waste1 @15:35:40.99 delay 30 seconds @15:35:44.05 Intake.select :close @15:35:44.21 SSV.dial :exhaust @15:35:44.98 Exhaust.select :open @15:35:45.15 SS.seek 0ml @15:35:53.92 Exhaust.select :close @15:35:54.02 SSV.dial CBV @15:35:54.07 CBV.dial SSV @15:35:54.82 Allowing 130s for 25.0ml of remaining 575.0ml @15:35:54.84 Intake.select :open @15:35:57.01 Intake @16.1psia, vacuum from 10.0 to 13.0psi @15:35:57.03 SS.reconfigure :inhaling 0.47ml/s @15:35:57.07 SS.seek 25ml @15:36:11.01 PBV.dial between PSV and waste1 @15:36:11.38 PSV.dial :air @15:36:11.85 PS.reconfigure :fast @15:36:11.91 PS.seek 2.5ml @15:36:35.94 PSV.dial :mixloop @15:36:36.41 delay 2 seconds @15:36:36.49 Vacuum too high -- waiting up to 40 seconds @15:36:36.50 SS.reconfigure :exhale @15:36:36.56 SS.hold @15:36:38.51 PSV.dial :air @15:36:39.13 delay 2 seconds @15:36:41.14 PSV.dial :mixloop @15:36:41.60 delay 2 seconds @15:36:43.61 PSV.dial :air @15:36:44.09 PS.seek 0ml @15:36:51.50 Intake @16.6psia, vacuum from 10.0 to 13.0psi @15:36:51.52 SS.reconfigure :inhaling 0.38ml/s @15:36:51.56 SS.seek 25ml @15:37:07.60 washCycleDA #1 of 3 @15:37:07.61 PSR.select :air @15:37:07.63 PSV.dial PSR @15:37:08.97 Pulling 0.5ml of PSR.wash @15:37:08.98 PSR.select :wash @15:37:09.10 PS.reconfigure :default @15:37:09.15 PS.seek 0.5ml @15:37:11.93 Stroke completed -- waiting for pressure to equalize ... @15:37:11.94 SS.reconfigure :exhale @15:37:11.99 SS.hold @15:37:15.06 delay 5 seconds @15:37:20.08 PSR.select :air @15:37:20.19 PS.seek 2ml @15:37:36.40 PTV.dial :puck @15:37:36.75 Intake.select :close @15:37:36.91 SSV.dial :exhaust @15:37:37.17 PSV.dial PTV @15:37:37.68 Exhaust.select :open @15:37:37.80 SS.seek 0ml @15:37:37.92 PBV.dial :waste1 @15:37:38.36 PS.seek 1.5ml @15:37:44.79 delay 3 seconds @15:37:46.83 Exhaust.select :close @15:37:46.94 SSV.dial CBV @15:37:47.04 CBV.dial SSV @15:37:47.79 Allowing 130s for 25.0ml of remaining 550.0ml @15:37:47.81 Intake.select :open @15:37:47.86 PBV.dial between PSV and waste1 @15:37:48.24 PS.seek 0ml @15:37:49.97 Intake @15.9psia, vacuum from 10.0 to 13.0psi @15:37:49.99 SS.reconfigure :inhaling 0.38ml/s @15:37:50.04 SS.seek 25ml @15:38:04.44 delay 2:00 @15:38:58.35 Stroke completed -- waiting for pressure to equalize ... @15:38:58.36 SS.reconfigure :exhale @15:38:58.41 SS.hold @15:39:33.19 Intake.select :close @15:39:33.35 SSV.dial :exhaust @15:39:34.13 Exhaust.select :open @15:39:34.25 SS.seek 0ml @15:39:43.13 Exhaust.select :close @15:39:43.24 SSV.dial CBV @15:39:43.28 CBV.dial SSV @15:39:44.05 Allowing 130s for 25.0ml of remaining 525.0ml @15:39:44.07 Intake.select :open @15:39:46.23 Intake @15.9psia, vacuum from 10.0 to 13.0psi @15:39:46.25 SS.reconfigure :inhaling 0.38ml/s @15:39:46.30 SS.seek 25ml @15:40:04.46 recoverPSRreagent08 @15:40:04.47 PTV.dial :puck @15:40:04.55 PBV.dial :waste1 @15:40:04.92 delay 30 seconds @15:40:34.95 PBV.dial between PSV and waste1 @15:40:35.33 PSR.select :air @15:40:35.35 PSV.dial PSR @15:40:36.11 PS.reconfigure :fast @15:40:36.16 PS.seek 2.5ml @15:40:46.08 Vacuum too high -- waiting up to 40 seconds @15:40:46.09 SS.reconfigure :exhale @15:40:46.15 SS.hold @15:41:00.15 PSV.dial PTV @15:41:00.92 delay 5 seconds @15:41:04.66 Intake @16.3psia, vacuum from 10.0 to 13.0psi @15:41:04.68 SS.reconfigure :inhaling 0.31ml/s @15:41:04.73 SS.seek 25ml @15:41:05.94 PSV.dial PSR @15:41:06.81 delay 5 seconds @15:41:11.83 PSV.dial PTV @15:41:12.58 delay 5 seconds @15:41:16.20 Stroke completed -- waiting for pressure to equalize ... @15:41:16.22 SS.reconfigure :exhale @15:41:16.26 SS.hold @15:41:17.59 PSV.dial :air @15:41:18.36 PS.seek 0ml @15:41:41.04 Intake.select :close @15:41:41.21 SSV.dial :exhaust @15:41:41.97 Exhaust.select :open @15:41:42.09 SS.seek 0ml @15:41:42.28 washCycleDA #2 of 3 @15:41:42.29 PSR.select :air @15:41:42.31 PSV.dial PSR @15:41:43.65 Pulling 0.5ml of PSR.wash @15:41:43.66 PSR.select :wash @15:41:43.78 PS.reconfigure :default @15:41:43.83 PS.seek 0.5ml @15:41:49.72 delay 5 seconds @15:41:50.91 Exhaust.select :close @15:41:51.02 SSV.dial CBV @15:41:51.07 CBV.dial SSV @15:41:51.83 Allowing 130s for 25.0ml of remaining 500.0ml @15:41:51.85 Intake.select :open @15:41:54.01 Intake @15.9psia, vacuum from 10.0 to 13.0psi @15:41:54.03 SS.reconfigure :inhaling 0.31ml/s @15:41:54.55 SS.seek 25ml @15:41:54.73 PSR.select :air @15:41:54.85 PS.seek 2ml @15:42:11.06 PTV.dial :puck @15:42:11.14 PSV.dial PTV @15:42:11.89 PBV.dial :waste1 @15:42:12.33 PS.seek 1.5ml @15:42:18.25 delay 3 seconds @15:42:21.27 PBV.dial between PSV and waste1 @15:42:21.64 PS.seek 0ml @15:42:37.97 delay 2:00 @15:43:21.50 Stroke completed -- waiting for pressure to equalize ... @15:43:21.51 SS.reconfigure :exhale @15:43:21.56 SS.hold @15:44:01.76 SS.reconfigure :default @15:44:01.81 SS.hold @15:44:01.88 Intake.select :close @15:44:02.04 ShallowSampler::Clogged in WCR -- Intake @16psia & Outlet @15psia won't equalize after 525.0ml @15:44:02.06 SSV.dial :exhaust @15:44:02.81 Exhaust.select :open @15:44:02.93 SS.reconfigure :exhale @15:44:02.98 SS.seek 0ml @15:44:12.14 Exhaust.select :close @15:44:12.26 Sampled 525.0ml @15:44:12.27 Drying puck before fixing for 5:00 @15:44:12.28 CBV.dial :waste2b @15:44:13.05 delay 1:00 @15:44:37.99 recoverPSRreagent08 @15:44:38.00 PTV.dial :puck @15:44:38.07 PBV.dial :waste1 @15:44:38.45 delay 30 seconds @15:45:08.47 PBV.dial between PSV and waste1 @15:45:08.85 PSR.select :air @15:45:08.87 PSV.dial PSR @15:45:09.64 PS.reconfigure :fast @15:45:09.69 PS.seek 2.5ml @15:45:13.06 CSV.dial :air @15:45:13.53 CS.reconfigure :fast @15:45:13.58 CS.seek 10ml @15:45:33.52 PSV.dial PTV @15:45:34.29 delay 5 seconds @15:45:37.94 Pumping wcr puck from tube 5 up to 35psi... @15:45:37.96 CBV.dial SSV @15:45:39.23 CBV.dial between CSV and waste2b @15:45:39.75 PSV.dial PSR @15:45:40.52 delay 5 seconds @15:45:40.67 CSV.dial between air and CTV @15:45:41.01 CS.seek 7.197ml @15:45:45.54 PSV.dial PTV @15:45:46.31 delay 5 seconds @15:45:48.19 CTV.dial CSV,avoiding: :air @15:45:48.22 CSV.dial CTV @15:45:49.07 CS.reconfigure :pressurize @15:45:49.12 CS.seek 0ml @15:45:51.32 PSV.dial :air @15:45:52.09 PS.seek 0ml @15:46:08.03 CSV.dial :air @15:46:08.47 CS.reconfigure :fast @15:46:08.52 CS.seek 10ml @15:46:15.46 washCycleDA #3 of 3 @15:46:15.47 PSR.select :air @15:46:15.49 PSV.dial PSR @15:46:16.82 Pulling 0.5ml of PSR.wash @15:46:16.83 PSR.select :wash @15:46:16.95 PS.reconfigure :default @15:46:16.99 PS.seek 0.5ml @15:46:22.96 delay 5 seconds @15:46:27.98 PSR.select :air @15:46:28.10 PS.seek 2ml @15:46:32.90 CSV.dial between air and CTV @15:46:33.23 CS.seek 4.446ml @15:46:44.33 PTV.dial :puck @15:46:44.41 PSV.dial PTV @15:46:45.29 PBV.dial :waste1 @15:46:45.72 PS.seek 1.5ml @15:46:46.66 CTV.dial CSV,avoiding: :air @15:46:46.68 CSV.dial CTV @15:46:47.08 CS.reconfigure :pressurize @15:46:47.13 CS.seek 0ml @15:46:51.68 delay 3 seconds @15:46:54.70 PBV.dial between PSV and waste1 @15:46:55.06 PS.seek 0ml @15:46:59.03 CSV.dial :air @15:46:59.49 CS.reconfigure :fast @15:46:59.54 CS.seek 10ml @15:47:11.18 delay 2:00 @15:47:24.24 CSV.dial between air and CTV @15:47:24.57 CS.seek 4.567ml @15:47:37.82 CTV.dial CSV,avoiding: :air @15:47:37.84 CSV.dial CTV @15:47:38.27 CS.reconfigure :pressurize @15:47:38.33 CS.seek 0ml @15:47:50.53 CSV.dial :air @15:47:50.97 CS.reconfigure :fast @15:47:51.02 CS.seek 10ml @15:48:15.30 CSV.dial between air and CTV @15:48:15.67 CS.seek 4.554ml @15:48:28.85 CTV.dial CSV,avoiding: :air @15:48:28.87 CSV.dial CTV @15:48:29.17 CS.reconfigure :pressurize @15:48:29.23 CS.seek 0ml @15:48:41.43 CSV.dial :air @15:48:41.89 CS.reconfigure :default @15:48:41.94 CS.hold @15:48:42.06 ShallowSampler::Error in MAIN -- Puck.from 5, :wcr reached only 18.5 of 35psi after pumping in 40ml of gas @15:48:42.08 Waiting to Resume from Checkpoint "Pumping wcr puck from tube 5 up to 35psi..." @15:48:42.10 BadNews.email "Puck.from 5, :wcr reached only 18.5 of 35psi after pumping in 40ml of gas",Subject: "ShallowSampler::Error in MAIN" @15:48:46.44 upsync @15:49:11.21 recoverPSRreagent08 @15:49:11.22 PTV.dial :puck @15:49:11.30 PBV.dial :waste1 @15:49:11.66 delay 30 seconds @15:49:41.69 PBV.dial between PSV and waste1 @15:49:42.05 PSR.select :air @15:49:42.07 PSV.dial PSR @15:49:42.82 PS.reconfigure :fast @15:49:42.88 PS.seek 2.5ml @15:50:06.99 PSV.dial PTV @15:50:07.75 delay 5 seconds @15:50:12.78 PSV.dial PSR @15:50:13.53 delay 5 seconds @15:50:19.00 PSV.dial PTV @15:50:19.82 delay 5 seconds @15:50:24.84 PSV.dial :air @15:50:25.73 PS.seek 0ml @15:50:49.08 Gate.power :camera,:ON @15:50:49.10 Mixing DA Substrates PSR.select :air @15:50:49.12 PSV.dial PSR @15:50:50.52 Pulling 0.25ml of PSR.sub1 @15:50:50.55 PSR.select :sub1 @15:50:50.76 PS.reconfigure :default @15:50:50.87 PS.seek 0.25ml @15:50:54.28 delay 5 seconds @15:50:59.30 Pulling 0.25ml of PSR.sub2 @15:50:59.31 PSR.select :sub2 @15:50:59.43 PS.seek 0.5ml @15:51:02.92 delay 5 seconds @15:51:07.94 PSR.select :air @15:51:08.06 PS.seek 2ml @15:51:24.20 PTV.dial :puck @15:51:24.28 PSV.dial PTV @15:51:25.08 PBV.dial :waste1 @15:51:25.52 PS.seek 1.5ml @15:51:31.49 delay 3 seconds @15:51:34.50 PBV.dial between PSV and waste1 @15:51:34.88 PS.seek 0ml @15:51:51.08 delay 10 seconds @15:52:01.11 recoverPSRreagent08 @15:52:01.12 PTV.dial :puck @15:52:01.19 PBV.dial :waste1 @15:52:01.56 delay 30 seconds @15:52:31.59 PBV.dial between PSV and waste1 @15:52:31.98 PSR.select :air @15:52:32.00 PSV.dial PSR @15:52:32.79 PS.reconfigure :fast @15:52:32.84 PS.seek 2.5ml @15:52:57.04 PSV.dial PTV @15:52:57.83 delay 5 seconds @15:53:02.85 PSV.dial PSR @15:53:03.62 delay 5 seconds @15:53:08.65 PSV.dial PTV @15:53:09.55 delay 5 seconds @15:53:14.57 PSV.dial :air @15:53:15.36 PS.seek 0ml @15:53:39.05 Additional air clear @15:53:39.06 PSR.select :air @15:53:39.07 PSV.dial PSR @15:53:40.43 Pulling 2ml of PSR.air @15:53:40.44 PSR.select :air @15:53:40.47 PS.reconfigure :default @15:53:40.51 PS.seek 2ml @15:54:01.82 delay 5 seconds @15:54:06.85 PTV.dial :puck @15:54:06.92 PSV.dial PTV @15:54:07.68 PBV.dial :waste1 @15:54:08.14 PS.seek 1.5ml @15:54:14.04 delay 3 seconds @15:54:17.06 PBV.dial between PSV and waste1 @15:54:17.44 delay 1 second @15:54:18.45 PSV.dial :waste1 @15:54:19.53 PS.seek 0ml @15:54:35.76 Processing Puck Heater at 28.1C before imaging @15:54:35.77 PH.coast @15:54:35.88 Imaging da puck in Processing Clamp while storing it to tube 3 @15:54:36.54 SC.reconfigure :tight @15:54:36.64 SC.seek 3 @15:54:43.40 SC.configure :limp @15:54:43.62 Elbow.reconfigure :tight @15:54:43.67 Elbow.seek 3 @15:54:45.44 Forearm.reconfigure :tight @15:54:45.49 Forearm.seek 3 + 450 counts @15:54:46.53 SE.reconfigure :pick @15:54:46.59 SE.seek :beamBreak @15:54:47.62 SE.configure :default @15:54:47.79 Elbow.reconfigure :limp @15:54:47.84 Forearm.reconfigure :loose @15:54:47.89 Forearm.seek 3 @15:54:48.18 Elbow.hold @15:54:48.25 Elbow.reconfigure :tight @15:54:48.35 SE.seek 31.1 Pucks below Top Plate @15:54:48.81 Forearm.force -20 @15:54:48.85 Forearm.seek 3 + 310 counts @15:54:49.24 Forearm.reconfigure :tight @15:54:49.29 Forearm.seek 3 + 450 counts @15:54:49.55 SE.seek 32.1 Pucks below Top Plate @15:54:50.21 Forearm.reconfigure :loose @15:54:50.26 Forearm.seek :retract @15:54:51.19 Elbow.seek PC @15:54:53.68 Elbow.reconfigure :loose @15:54:53.79 Forearm.seek PC @15:54:54.76 Hand.close @15:54:55.37 PC.seek :open @15:55:00.18 Forearm.seek :retract @15:55:01.19 Elbow.reconfigure :tight @15:55:01.24 Elbow.seek 3 @15:55:04.37 Forearm.reconfigure :tight @15:55:04.42 Forearm.seek 3 + 450 counts @15:55:05.42 Hand.open @15:55:06.06 SE.seek 31.75 Pucks below Top Plate @15:55:06.32 SE.reconfigure :seal @15:55:06.38 SE.seek 30.79 Pucks below Top Plate @15:55:06.92 Lights, camera, ACTION! @15:55:06.96 PuckCamera: snap -bin=2,2 -auto /var/log/esp/da23dec0115h1000ml.tif @15:55:26.43 Waiting >20 seconds for da to relinquish FlushPuck @15:55:26.91 SX HX9/16 (USB:12): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 16.629 seconds /var/log/esp/da23dec0115h1000ml.tif: TIFF Upload Complete @15:55:26.94 PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da23dec0115h1000ml15s.tif @15:55:42.55 SX HX9/16 (USB:12): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15 seconds /var/log/esp/midres/da23dec0115h1000ml15s.tif: TIFF Upload Complete @15:55:42.58 PuckCamera: snap -bin=2,2 20.0 /var/log/esp/midres/da23dec0115h1000ml20s.tif @15:56:03.19 SX HX9/16 (USB:12): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 20 seconds /var/log/esp/midres/da23dec0115h1000ml20s.tif: TIFF Upload Complete @15:56:03.20 Gate.power :camera,:OFF @15:56:03.23 DAfans.email "16.629 sec auto-exposure",Subject: "da23dec0115h1000ml images!" @15:56:03.69 Elbow.seek 3 @15:56:03.94 Forearm.seek 3 + 450 counts @15:56:04.03 SE.reconfigure :gently @15:56:04.09 SE.seek 31.5 Pucks below Top Plate @15:56:04.75 Elbow.reconfigure :limp @15:56:04.82 Forearm.reconfigure :loose @15:56:04.87 Forearm.seek 3 @15:56:05.15 Elbow.hold @15:56:05.23 Elbow.reconfigure :tight @15:56:05.29 SE.reconfigure :default @15:56:05.34 SE.seek 31.69 Pucks below Top Plate @15:56:05.81 Forearm.force -20 @15:56:05.86 Forearm.seek 3 + 296 counts @15:56:06.44 Forearm.reconfigure :tight @15:56:06.54 Forearm.seek 3 + 450 counts @15:56:06.96 SE.seek :down @15:56:07.53 SE.stop @15:56:08.22 Forearm.reconfigure :loose @15:56:09.46 Forearm.seek :retract @15:56:10.63 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @15:56:11.12 Elbow.seek FlushPuck::Garage @15:56:13.05 Elbow.reconfigure :loose @15:56:13.26 Forearm.seek :garage @15:56:14.29 Hand.close @15:56:15.13 Forearm.seek :retract @15:56:16.23 Elbow.reconfigure :tight @15:56:16.32 Elbow.seek PC @15:56:17.67 Elbow.reconfigure :loose @15:56:17.87 Forearm.reconfigure :tight @15:56:17.98 Forearm.seek PC @15:56:19.55 PC.seek :closed @15:56:24.35 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.62 @15:56:24.52 Hand.open @15:56:25.27 Forearm.reconfigure :loose @15:56:25.36 Forearm.seek :retract @15:56:26.35 PSR.select :air @15:56:26.39 PSV.dial PSR @15:56:26.93 Pulling 0.5ml of PSR.flush @15:56:26.97 PSR.select :flush @15:56:27.14 PS.seek 0.5ml @15:56:33.07 delay 5 seconds @15:56:35.88 upsync @15:56:38.09 PSR.select :air @15:56:38.21 PS.seek 2.5ml @15:56:59.45 delay 1 second @15:57:00.47 PRV.dial :waste2,avoiding: :ab2 @15:57:02.34 PTV.dial :PRVmixing @15:57:03.66 PSV.dial :mixloop @15:57:04.83 PS.seek :empty @15:57:31.16 PSR.select :air @15:57:31.57 PSV.dial PSR @15:57:32.70 Pulling 0.5ml of PSR.flush @15:57:32.71 PSR.select :flush @15:57:32.83 PS.seek 0.5ml @15:57:38.76 delay 5 seconds @15:57:43.78 PSR.select :air @15:57:43.90 PS.seek 2.5ml @15:58:05.23 delay 1 second @15:58:06.25 PBV.dial :waste1 @15:58:06.60 PTV.dial :mixing @15:58:07.35 PSV.dial :mixloop @15:58:08.42 PS.seek :empty @15:58:34.76 PSV.dial PSR @15:58:35.80 PSR.select :flush @15:58:35.93 PS.seek 1ml @15:58:46.94 PSR.select :air @15:58:47.06 PS.seek 2.5ml @15:59:03.19 PSV.dial PTV @15:59:03.96 PTV.dial :puck @15:59:04.69 PBV.dial :waste1 @15:59:04.78 PS.seek 0ml @15:59:31.16 PSV.dial PSR @15:59:31.93 PSR.select :flush @15:59:32.05 PS.seek 0.5ml @15:59:38.00 PSR.select :air @15:59:38.12 PS.seek 2.5ml @15:59:59.32 PSV.dial PTV @16:00:00.08 PBV.dial PSV @16:00:00.65 PS.seek 0ml @16:00:27.03 PSV.dial PBV @16:00:27.51 PS.seek 2.5ml @16:00:53.91 PSV.dial :waste1 @16:00:54.65 PS.seek 0ml @16:01:21.19 PSV.dial PSR @16:01:21.66 PSR.select :air @16:01:21.69 PS.seek 2.5ml @16:01:48.06 PSV.dial PTV @16:01:48.80 PBV.dial PSV @16:01:48.99 PS.seek 0ml @16:02:15.31 PSV.dial PBV @16:02:15.79 PS.seek 2.5ml @16:02:42.16 PSV.dial :waste1 @16:02:42.90 PS.seek 0ml @16:03:09.28 PSV.dial PSR @16:03:09.75 PSR.select :flush @16:03:09.87 PS.seek 1ml @16:03:21.50 PSR.select :air @16:03:21.62 PS.seek 2.5ml @16:03:37.81 PSV.dial PTV @16:03:38.55 PTV.dial :puck @16:03:38.63 PBV.dial :waste1 @16:03:39.18 PS.seek 0ml @16:04:05.59 PSV.dial PSR @16:04:06.34 PSR.select :flush @16:04:06.47 PS.seek 0.5ml @16:04:12.37 PSR.select :air @16:04:12.49 PS.seek 2.5ml @16:04:33.72 PSV.dial PTV @16:04:34.48 PBV.dial PSV @16:04:35.05 PS.seek 0ml @16:05:01.37 PSV.dial PBV @16:05:01.85 PS.seek 2.5ml @16:05:28.22 PSV.dial :waste1 @16:05:28.96 PS.seek 0ml @16:05:55.44 PSV.dial PSR @16:05:55.90 PSR.select :air @16:05:55.93 PS.seek 2.5ml @16:06:22.28 PSV.dial PTV @16:06:23.02 PBV.dial PSV @16:06:23.21 PS.seek 0ml @16:06:49.59 PSV.dial PBV @16:06:50.07 PS.seek 2.5ml @16:07:16.62 PSV.dial :waste1 @16:07:17.36 PS.seek 0ml @16:07:43.84 Garaging FlushPuck in Processing Clamp @16:07:43.91 Elbow.reconfigure :tight @16:07:43.96 Elbow.seek PC @16:07:44.09 Elbow.reconfigure :loose @16:07:44.19 Forearm.seek PC @16:07:45.16 Hand.close @16:07:45.74 PC.seek :open @16:07:50.46 Forearm.seek :retract @16:07:51.41 Elbow.reconfigure :tight @16:07:51.46 Elbow.seek :garage @16:07:52.81 Elbow.reconfigure :loose @16:07:53.29 Forearm.reconfigure :tight @16:07:53.40 Forearm.seek :garage @16:07:54.44 Hand.open @16:07:55.17 Forearm.reconfigure :loose @16:07:55.22 Forearm.seek :retract @17:14:44.23 Session Begun @17:14:54.94 -> MainThread.resume @17:14:54.96
Resuming Thread (suspended since 15:45:37) # @17:14:55.01 Pumping wcr puck from tube 5 up to 35psi... @17:14:55.04 CBV.dial SSV @17:14:56.48 CBV.dial between CSV and waste2b @17:14:57.45 CSV.dial between air and CTV @17:14:57.79 CS.reconfigure :fast @17:14:57.87 CS.seek 5.888ml @17:15:13.11 CTV.dial CSV,avoiding: :air @17:15:13.13
CSV.dial CTV @17:15:13.46 CS.reconfigure :pressurize @17:15:13.54 CS.seek 0ml @17:15:29.19 CSV.dial :air @17:15:29.67 CS.reconfigure :fast @17:15:29.74 CS.seek 10ml @17:15:54.06 CSV.dial between air and CTV @17:15:54.42 CS.seek 5.91ml @17:16:04.45 CTV.dial CSV,avoiding: :air @17:16:04.48
CSV.dial CTV @17:16:04.81 CS.reconfigure :pressurize @17:16:04.88 CS.seek 0ml @17:16:20.60 CSV.dial :air @17:16:21.07 CS.reconfigure :fast @17:16:21.14 CS.seek 10ml @17:16:45.53 CSV.dial between air and CTV @17:16:45.87 CS.seek 4.429ml @17:16:59.32 CTV.dial CSV,avoiding: :air @17:16:59.34
CSV.dial CTV @17:16:59.68 CS.reconfigure :pressurize @17:16:59.75 CS.seek 0ml @17:17:11.75 CSV.dial :air @17:17:12.23 CS.reconfigure :fast @17:17:12.30 CS.seek 10ml @17:17:36.53 CSV.dial between air and CTV @17:17:36.87 CS.seek 4.46ml @17:17:50.23 CTV.dial CSV,avoiding: :air @17:17:50.26
CSV.dial CTV @17:17:50.60 CS.reconfigure :pressurize @17:17:50.67 CS.seek 0ml @17:18:02.69 CSV.dial :air @17:18:03.16 CS.reconfigure :default @17:18:03.23 CS.hold @17:18:03.38 ShallowSampler::Error in MAIN -- Puck.from 5, :wcr reached only 19.5 of 35psi after pumping in 40ml of gas @17:18:03.39 Waiting to Resume from Checkpoint "Pumping wcr puck from tube 5 up to 35psi..." @17:18:03.42 BadNews.email "Puck.from 5, :wcr reached only 19.5 of 35psi after pumping in 40ml of gas",Subject: "ShallowSampler::Error in MAIN" @17:18:08.23 upsync @17:18:32.00 -> Flush FlushPuck is in FlushPuck::Garage @17:18:34.47 -> CC Collection Clamp contains wcr puck from tube 5 @17:18:38.42 -> PC Processing Clamp open @17:20:25.42 -> Puck.count @17:20:25.52 SE.seek :down @17:20:25.61 SE.stop @17:20:25.73 SC.reconfigure :tight @17:20:25.80 SC.seek 1 @17:20:32.47 SC.configure :limp @17:20:32.72 Elbow.reconfigure :tight @17:20:32.80 Elbow.seek 1 @17:20:34.81 Forearm.reconfigure :tight @17:20:34.94 Forearm.seek 1 + 450 counts @17:20:35.98 SE.reconfigure :place @17:20:36.06 SE.seek :beamBreak @17:20:37.21 SE.configure :default @17:20:37.35 SE.seek :down @17:20:37.84 SE.stop @17:20:38.48 Forearm.reconfigure :loose @17:20:38.55 Forearm.seek :retract @17:20:39.51 SC.reconfigure :tight @17:20:39.58 SC.seek 2 @17:20:43.21 SC.configure :limp @17:20:43.39 Elbow.seek 2 @17:20:43.89 Forearm.reconfigure :tight @17:20:43.96 Forearm.seek 2 + 450 counts @17:20:45.08 SE.reconfigure :place @17:20:45.16 SE.seek :beamBreak @17:20:46.40 SE.configure :default @17:20:46.54 SE.seek :down @17:20:47.02 SE.stop @17:20:47.67 Forearm.reconfigure :loose @17:20:47.74 Forearm.seek :retract @17:20:48.69 SC.reconfigure :tight @17:20:48.76 SC.seek 3 @17:20:52.32 SC.configure :limp @17:20:52.50 Elbow.seek 3 @17:20:52.78 Forearm.reconfigure :tight @17:20:52.86 Forearm.seek 3 + 450 counts @17:20:53.90 SE.reconfigure :place @17:20:53.98 SE.seek :beamBreak @17:20:55.12 SE.configure :default @17:20:55.25 SE.seek :down @17:20:55.69 SE.stop @17:20:56.38 Forearm.reconfigure :loose @17:20:56.46 Forearm.seek :retract @17:20:57.42 SC.reconfigure :tight @17:20:57.49 SC.seek 4 @17:21:01.10 SC.configure :limp @17:21:01.27 Elbow.seek 4 @17:21:01.90 Forearm.reconfigure :tight @17:21:01.98 Forearm.seek 4 + 450 counts @17:21:03.08 SE.reconfigure :place @17:21:03.16 SE.seek :beamBreak @17:21:06.51 SE.configure :default @17:21:06.65 SE.seek :down @17:21:07.78 Forearm.reconfigure :loose @17:21:07.85 Forearm.seek :retract @17:21:09.45 SE.stop @17:21:09.51 SC.reconfigure :tight @17:21:09.58 SC.seek 5 @17:21:13.02 SC.configure :limp @17:21:13.20 Elbow.seek 5 @17:21:13.56 Forearm.reconfigure :tight @17:21:13.64 Forearm.seek 5 + 450 counts @17:21:14.75 SE.reconfigure :place @17:21:14.84 SE.seek :beamBreak @17:21:16.05 SE.configure :default @17:21:16.18 SE.seek :down @17:21:16.69 SE.stop @17:21:17.31 Forearm.reconfigure :loose @17:21:17.38 Forearm.seek :retract @17:21:18.33 SC.reconfigure :tight @17:21:18.40 SC.seek 6 @17:21:21.99 SC.configure :limp @17:21:22.17 Elbow.seek 6 @17:21:22.38 Forearm.reconfigure :tight @17:21:22.46 Forearm.seek 6 + 450 counts @17:21:23.62 SE.reconfigure :place @17:21:23.70 SE.seek :beamBreak @17:21:24.90 SE.configure :default @17:21:25.03 SE.seek :down @17:21:25.45 SE.stop @17:21:26.16 Forearm.reconfigure :loose @17:21:26.23 Forearm.seek :retract @17:21:27.18 SC.reconfigure :tight @17:21:27.25 SC.seek 7 @17:21:30.80 SC.configure :limp @17:21:30.98 Elbow.seek 7 @17:21:32.01 Forearm.reconfigure :tight @17:21:32.08 Forearm.seek 7 + 450 counts @17:21:33.15 SE.reconfigure :place @17:21:33.23 SE.seek :beamBreak @17:21:34.54 SE.configure :default @17:21:34.68 SE.seek :down @17:21:35.33 SE.stop @17:21:35.82 Forearm.reconfigure :loose @17:21:35.89 Forearm.seek :retract {1 => 33, 2 => 33, 3 => 33, 4 => 0, 5 => 32, 6 => 33, 7 => 31} @17:23:46.10 -> CC.unloadPuck 4, :WCR @17:23:46.19 Puck::Warning in dave -- Unloading Puck.from 5, :wcr to Storage Carousel @17:23:46.21 Storing WCR puck in Collection Clamp to tube 4 @17:23:46.42 SC.reconfigure :tight @17:23:46.49 SC.seek 4 @17:23:56.33 SC.configure :limp @17:23:56.50 SE.reconfigure :place @17:23:56.63 SE.seek :beamBreak @17:23:56.72 Elbow.seek CC @17:23:58.29 Elbow.reconfigure :loose @17:23:58.41 Forearm.seek CC @17:23:59.42 Hand.close @17:23:59.56 SE.configure :default @17:23:59.63 SE.seek :place @17:24:00.05 CC.seek :open @17:24:04.85 Forearm.seek :retract @17:24:05.85 Elbow.reconfigure :tight @17:24:05.92 Elbow.seek 4 @17:24:07.71 Elbow.reconfigure :limp @17:24:07.78 Forearm.seek 4 @17:24:08.81 Elbow.hold @17:24:08.90 Elbow.reconfigure :tight @17:24:08.97 Hand.open @17:24:09.68 Forearm.force -20 @17:24:09.74 Forearm.seek 4 + 289 counts @17:24:10.05 Forearm.reconfigure :tight @17:24:10.12 Forearm.seek 4 + 450 counts @17:24:10.38 SE.seek :down @17:24:11.96 Forearm.reconfigure :loose @17:24:12.04 Forearm.seek :retract @17:24:13.17 SE.stop @17:24:31.65 -> CC Collection Clamp open @17:24:36.44 -> Puck.count @17:24:36.53 SE.seek :down @17:24:36.69 SE.stop @17:24:36.81 SC.reconfigure :tight @17:24:36.88 SC.seek 1 @17:24:46.98 SC.configure :limp @17:24:47.26 Elbow.seek 1 @17:24:47.61 Forearm.reconfigure :tight @17:24:47.68 Forearm.seek 1 + 450 counts @17:24:48.74 SE.reconfigure :place @17:24:48.82 SE.seek :beamBreak @17:24:49.96 SE.configure :default @17:24:50.09 SE.seek :down @17:24:50.55 SE.stop @17:24:51.22 Forearm.reconfigure :loose @17:24:51.29 Forearm.seek :retract @17:24:52.25 SC.reconfigure :tight @17:24:52.32 SC.seek 2 @17:24:55.92 SC.configure :limp @17:24:56.10 Elbow.seek 2 @17:24:56.31 Forearm.reconfigure :tight @17:24:56.38 Forearm.seek 2 + 450 counts @17:24:57.51 SE.reconfigure :place @17:24:57.59 SE.seek :beamBreak @17:24:58.83 SE.configure :default @17:24:58.96 SE.seek :down @17:24:59.45 SE.stop @17:25:00.09 Forearm.reconfigure :loose @17:25:00.17 Forearm.seek :retract @17:25:01.16 SC.reconfigure :tight @17:25:01.24 SC.seek 3 @17:25:04.86 SC.configure :limp @17:25:05.04 Elbow.seek 3 @17:25:05.92 Forearm.reconfigure :tight @17:25:06.07 Forearm.seek 3 + 450 counts @17:25:07.11 SE.reconfigure :place @17:25:07.20 SE.seek :beamBreak @17:25:08.33 SE.configure :default @17:25:08.47 SE.seek :down @17:25:08.92 SE.stop @17:25:09.60 Forearm.reconfigure :loose @17:25:09.67 Forearm.seek :retract @17:25:10.64 SC.reconfigure :tight @17:25:10.71 SC.seek 4 @17:25:14.25 SC.configure :limp @17:25:14.43 Elbow.seek 4 @17:25:14.92 Forearm.reconfigure :tight @17:25:15.00 Forearm.seek 4 + 450 counts @17:25:16.04 SE.reconfigure :place @17:25:16.13 SE.seek :beamBreak @17:25:19.33 SE.configure :default @17:25:19.46 SE.seek :down @17:25:20.59 Forearm.reconfigure :loose @17:25:20.66 Forearm.seek :retract @17:25:22.11 SE.stop @17:25:22.17 SC.reconfigure :tight @17:25:22.24 SC.seek 5 @17:25:25.69 SC.configure :limp @17:25:25.87 Elbow.seek 5 @17:25:26.25 Forearm.reconfigure :tight @17:25:26.32 Forearm.seek 5 + 450 counts @17:25:27.31 SE.reconfigure :place @17:25:27.39 SE.seek :beamBreak @17:25:28.58 SE.configure :default @17:25:28.71 SE.seek :down @17:25:29.23 SE.stop @17:25:29.85 Forearm.reconfigure :loose @17:25:29.92 Forearm.seek :retract @17:25:30.91 SC.reconfigure :tight @17:25:30.99 SC.seek 6 @17:25:34.94 SC.configure :limp @17:25:35.20 Elbow.seek 6 @17:25:35.41 Forearm.reconfigure :tight @17:25:35.49 Forearm.seek 6 + 450 counts @17:25:36.55 SE.reconfigure :place @17:25:36.63 SE.seek :beamBreak @17:25:37.82 SE.configure :default @17:25:37.95 SE.seek :down @17:25:38.36 SE.stop @17:25:39.08 Forearm.reconfigure :loose @17:25:39.16 Forearm.seek :retract @17:25:40.13 SC.reconfigure :tight @17:25:40.20 SC.seek 7 @17:25:43.73 SC.configure :limp @17:25:43.91 Elbow.seek 7 @17:25:44.46 Forearm.reconfigure :tight @17:25:44.54 Forearm.seek 7 + 450 counts @17:25:45.58 SE.reconfigure :place @17:25:45.66 SE.seek :beamBreak @17:25:46.99 SE.configure :default @17:25:47.12 SE.seek :down @17:25:47.73 SE.stop @17:25:48.25 Forearm.reconfigure :loose @17:25:48.32 Forearm.seek :retract {1 => 33, 2 => 33, 3 => 33, 4 => 1, 5 => 32, 6 => 33, 7 => 31} @17:26:10.74 -> purgeIntake @17:26:10.76 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @17:26:10.97 Elbow.seek FlushPuck::Garage @17:26:12.78 Elbow.reconfigure :loose @17:26:12.90 Forearm.seek :garage @17:26:13.92 Hand.close @17:26:14.67 Forearm.seek :retract @17:26:15.71 Elbow.reconfigure :tight @17:26:15.79 Elbow.seek CC @17:26:18.75 Elbow.reconfigure :loose @17:26:18.86 Forearm.reconfigure :tight @17:26:18.93 Forearm.seek CC @17:26:20.67 CC.seek :closed @17:26:25.23 Collection Clamp onSpring/offSpring for 14/27 samples, on/off torque 2.37 @17:26:25.32 Hand.open @17:26:25.98 Forearm.reconfigure :loose @17:26:26.05 Forearm.seek :retract @17:26:27.07 Purging external Intake @17:26:27.21 CSV.dial :flush @17:26:27.69 CS.reconfigure :fast @17:26:27.76 CS.seek 10ml @17:26:52.21 delay 5 seconds @17:26:57.24 CSV.dial CBV @17:26:57.27 CBV.dial CSV @17:26:58.07 CTV.dial :intake @17:26:58.84 Intake.select :open @17:26:58.97 CS.seek 0ml @17:27:22.55 Intake.select :close @17:27:22.68 CTV.dial :air @17:27:23.46 Garaging FlushPuck in Collection Clamp @17:27:23.56 Elbow.reconfigure :tight @17:27:23.63 Elbow.seek CC @17:27:23.90 Elbow.reconfigure :loose @17:27:24.02 Forearm.seek CC @17:27:24.98 Hand.close @17:27:25.68 CC.seek :open @17:27:30.33 Forearm.seek :retract @17:27:31.33 Elbow.reconfigure :tight @17:27:31.40 Elbow.seek :garage @17:27:34.39 Elbow.reconfigure :loose @17:27:34.51 Forearm.reconfigure :tight @17:27:34.58 Forearm.seek :garage @17:27:35.66 Hand.open @17:27:36.36 Forearm.reconfigure :loose @17:27:36.43 Forearm.seek :retract FlushPuck::Garage @17:28:02.68 -> CS.flush @17:28:02.70 NoMethodError in dave -- undefined method `flush' for CS:Syringe @17:28:08.26 -> Flush FlushPuck is in FlushPuck::Garage @17:28:10.16 -> CC Collection Clamp open @17:28:12.02 -> PC Processing Clamp open @17:28:18.08 -> exit @17:28:18.10 Session Ended @20:30:25.65 Session Begun @20:30:39.99 -> abort! 'mission complete' @20:30:40.00 CmdServer::Client::Error in dave -- Aborting Mission: mission complete @20:30:40.04 BadNews.email "mission complete",Subject: "DA_WCR_120123 ABORTED!" # @20:30:44.37 upsync @20:30:44.48 Session Ended + ESPwhich DA_WCR_120123b.rb + fn=DA_WCR_120123b.rb + IFS=: + set /home/esp/esp2/mission . + fullPath=/home/esp/esp2/mission/DA_WCR_120123b.rb + [ -f /home/esp/esp2/mission/DA_WCR_120123b.rb ] + realpath /home/esp/esp2/mission/DA_WCR_120123b.rb + return 0 + scriptPath=/home/esp/esp2local/mission/DA_WCR_120123b.rb + exec ruby /home/esp/esp2/lib/esp.rb /home/esp/esp2local/mission/DA_WCR_120123b.rb @20:54:34.82UTC01-Dec-23
ESP2 v3.42 I2C Gateway v3.20 for core bus @20:54:37.43 Configuration for short puck jake 1/11/21 by brent@mbari.org /home/esp/esp2/type/shallow/jake/configure.rb @20:54:41.38 Polling Can every 10:00 @20:54:41.53 Cleanly exited at 20:33:55UTC01-Dec-23 @20:54:41.57 Loading /home/esp/esp2local/mission/DA_WCR_120123b.rb mission @20:54:41.63 GoodNews.email "Starting DA_WCR_120123b w/pucks in tube 5\n{1 => 33,\n 2 => 33,\n 3 => 33,\n 4 => 1,\n 5 => 32,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@20:54:41, 31% flow, 16.4C, 32% humidity, 15.1psia, 13.759V, 0.248A, 125.414Ah, 3.41W",Subject: "DA_WCR_120123b begun" @20:54:41.66
Preparing to sleep until + 00:00:05 @20:54:41.70 Asleep til + 00:00:05 @20:54:41.72 Continuing at 20:54 @20:54:42.01 Gate.power :core,:OFF @20:54:42.02 Gate.power :raw,:OFF @20:54:42.03 Shutdown @20:54:45.03 GoodNews.email "Then, in tube 5: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_120123b.rb:14\n{1 => 33,\n 2 => 33,\n 3 => 31,\n 4 => 1,\n 5 => 32,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@20:54:41, 31% flow, 16.5C, 32% humidity, 15.1psia, 13.759V, 0.254A, 125.414Ah, 3.50W",Subject: "Asleep until 20:54" @20:54:46.36 upsync @20:54:46.63
Powering core bus @20:54:48.86 upsync @20:54:52.20
Gate.power :raw,:ON @20:54:53.02 CS.configure :default @20:54:53.07 PS.configure :default @20:54:53.12 SS.configure :default @20:54:53.17 CC.configure :default @20:54:53.22 PC.configure :default @20:54:53.29 Forearm.configure :loose @20:54:53.35 Elbow.configure :loose @20:54:53.42 SC.configure :loose @20:54:53.47 SE.configure :default @20:54:53.52 CH.configure :default @20:54:53.55 PH.configure :default @20:54:53.59 All dwarf controllers running firmware version 3.85 @20:54:53.99 Valve.reset @20:54:54.25 Intake.select :close @20:54:54.32 Exhaust.select :close @20:54:54.36 CTV.dial :air @20:54:54.45 PSV.dial :air @20:54:54.58 CSV.dial :waste1 @20:54:54.60
Forearm.home.seek :retract @20:54:55.07 SSV.dial :spit,avoiding: :exhaust @20:54:55.41 Hand.close @20:54:55.44
Elbow.reconfigure :loose @20:54:55.53 Elbow.jog 1500 @20:54:55.61 PS.home.seek 0ml @20:54:55.96 CS.reconfigure :fast @20:54:55.99 SS.home.seek 0ml @20:54:56.10 CS.home.seek 0ml @20:54:56.17
Elbow.reconfigure :loose @20:54:56.23 Elbow.home.seek :clear @20:54:59.55 SE.home.seek :down @20:55:00.18 SC.reconfigure :tight @20:55:00.23 SC.home.seek :initial @20:55:03.84 PS.stop @20:55:28.62
SC.configure :limp @20:55:28.67 Forearm.seek :retract @20:55:28.74 Elbow.seek :hammer @20:55:29.75 Forearm.reconfigure :tight @20:55:29.80 Forearm.seek retracted @20:55:30.07 PC.home.seek :open @20:55:30.10
CC.home.seek :open @20:55:33.59 Notice.email "At 20:55:33 began in tube 5: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_120123b.rb:14\n{1 => 33,\n 2 => 33,\n 3 => 31,\n 4 => 1,\n 5 => 32,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@20:55:33, 31% flow, 16.5C, 32% humidity, 15.1psia, 13.749V, 0.302A, 125.420Ah, 4.16W",Subject: "Started DA_WCR_120123b phase initialPurge; da [1000, 1000] #da and wsr are run simultanelously" @20:55:33.62
Phase [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] @20:55:33.64 Starting initialPurge @20:55:33.65 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @20:55:33.72 Hand.open @20:55:34.44 Elbow.reconfigure :tight @20:55:34.51 Elbow.seek FlushPuck::Garage @20:55:36.52 Elbow.reconfigure :loose @20:55:36.67 Forearm.reconfigure :loose @20:55:36.72 Forearm.seek :garage @20:55:37.63 upsync @20:55:37.68
Hand.close @20:55:38.45 Forearm.seek :retract @20:55:39.46 Elbow.reconfigure :tight @20:55:39.51 Elbow.seek CC @20:55:42.47 Elbow.reconfigure :loose @20:55:42.57 Forearm.reconfigure :tight @20:55:42.62 Forearm.seek CC @20:55:43.86 CC.seek :closed @20:55:48.41 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.49 @20:55:48.47 Hand.open @20:55:49.13 Forearm.reconfigure :loose @20:55:49.17 Forearm.seek :retract @20:55:50.21 Flushing the Collection Valve::Manifold @20:55:50.34 CSV.dial CSR @20:55:51.63 Pulling 5ml of CSR.flush CSR.select :flush @20:55:51.75 CS.reconfigure :default @20:55:51.80 CS.seek 5ml @20:56:05.83 delay 5 seconds @20:56:10.85 CSR.select :air @20:56:11.01 CS.reconfigure :fast @20:56:11.06 CS.seek 7.501ml @20:56:17.83 CSV.dial :waste @20:56:19.13 CS.reconfigure :default @20:56:19.18 CS.seek 0ml @20:56:39.27 Purging external Intake @20:56:39.39 CSV.dial :flush @20:56:39.83 CS.reconfigure :fast @20:56:39.88 CS.seek 10ml @20:57:04.23 delay 5 seconds @20:57:09.26 CSV.dial CBV @20:57:09.29
CBV.dial CSV @20:57:10.03 CTV.dial :intake @20:57:10.83 Intake.select :open @20:57:10.93 CS.seek 0ml @20:57:34.45 Intake.select :close @20:57:34.56 CTV.dial :air @20:57:35.35 Flushing upper manifold @20:57:35.48 CSV.dial :flush @20:57:36.33 CS.seek 5ml @20:57:49.36 delay 5 seconds @20:57:54.38 CSV.dial :air @20:57:54.90 CS.seek 7.001ml @20:58:00.42 CSV.dial CBV @20:58:00.44
CBV.dial CSV @20:58:01.47 CTV.dial :air @20:58:01.55 CS.seek 0ml @20:58:18.14 delay 7 seconds @20:58:25.17 CS.seek 3ml @20:58:33.23 CSV.dial :waste @20:58:33.69 CS.seek 0ml @20:58:41.56 CSV.dial :flush @20:58:42.00 CS.seek 3ml @20:58:50.04 delay 5 seconds @20:58:55.06 CSV.dial :air @20:58:55.57 CS.seek 5ml @20:59:01.26 CSV.dial :air @20:59:01.44 CS.seek 0ml @20:59:13.64 delay 7 seconds @20:59:20.67 CS.seek 3ml @20:59:28.76 CSV.dial :waste @20:59:29.51 CS.seek 0ml @20:59:37.27 Garaging FlushPuck in Collection Clamp @20:59:37.34 Elbow.reconfigure :tight @20:59:37.38 Elbow.seek CC @20:59:37.71 Elbow.reconfigure :loose @20:59:37.81 Forearm.seek CC @20:59:38.86 Hand.close @20:59:39.44 CC.seek :open @20:59:44.46 Forearm.seek :retract @20:59:45.46 Elbow.reconfigure :tight @20:59:45.50 Elbow.seek :garage @20:59:48.42 Elbow.reconfigure :loose @20:59:48.52 Forearm.reconfigure :tight @20:59:48.56 Forearm.seek :garage @20:59:49.56 Hand.open @20:59:50.25 Forearm.reconfigure :loose @20:59:50.30 Forearm.seek :retract @20:59:51.32 da sampling at most 1000ml, wcr at most 1000ml @20:59:51.35 Loading sh1 puck from tube 5 into Collection Clamp @20:59:51.37 DAfans.email "Started da in tube 5 at 20:59:51UTC01-Dec-23\n{1 => 33,\n 2 => 33,\n 3 => 31,\n 4 => 1,\n 5 => 32,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@20:55:33, 31% flow, 16.5C, 32% humidity, 15.1psia, 13.749V, 0.302A, 125.420Ah, 4.16W",Subject: "da sampling at most 1000ml, wcr at most 1000ml" @20:59:51.64
SC.reconfigure :tight @20:59:51.69 SC.seek 5 @20:59:55.25 SC.configure :limp @20:59:55.36 upsync @20:59:55.52
Elbow.reconfigure :tight @20:59:55.58 Elbow.seek 5 @20:59:57.64 Forearm.reconfigure :tight @20:59:57.69 Forearm.seek 5 + 450 counts @20:59:58.72 SE.reconfigure :pick @20:59:58.78 SE.seek :beamBreak @20:59:59.86 SE.configure :default @21:00:00.02 Elbow.seek 5 @21:00:00.30 Forearm.seek 5 + 300 counts @21:00:00.55 Forearm.reconfigure :loose @21:00:00.60 Hand.close @21:00:01.20 SE.seek :down @21:00:01.41
Forearm.seek :retract @21:00:02.00 SE.stop @21:00:02.77
Elbow.seek CC @21:00:04.40 Elbow.reconfigure :loose @21:00:04.50 Forearm.reconfigure :tight @21:00:04.55 Forearm.seek CC @21:00:05.79 CC.seek :closed @21:00:10.30 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.35 @21:00:10.31 Hand.open @21:00:11.00 Forearm.reconfigure :loose @21:00:11.04 Forearm.seek :retract @21:00:12.00 collectSample 1000ml w/bubble point of 24psi @21:00:12.01 Priming 7ml @21:00:12.02 Intake.select :close @21:00:12.04 Exhaust.select :close @21:00:12.05 SS.hold @21:00:12.26 Calibrating pressure and temperature sensors @21:00:12.29 CTV.dial :air @21:00:12.36 SSV.dial :spit,avoiding: :exhaust @21:00:12.55 SS.reconfigure :fast @21:00:12.60 SS.seek 5ml @21:00:15.45 SSV.dial CBV @21:00:15.47
CBV.dial SSV @21:00:16.82 delay 3.8 seconds @21:00:20.75 Sensor limits: Intake max 101.0psia; Outlet max 95.2psia @21:00:20.76 SSV.dial :spit @21:00:22.09 SS.seek 0ml @21:00:24.94 SSV.dial CBV @21:00:26.26 CTV.dial :intake @21:00:27.05 SSV.dial CBV @21:00:27.07
CBV.dial SSV @21:00:27.29 Allowing 40s for 7.0ml of remaining 7.0ml @21:00:27.30 Intake.select :open @21:00:29.47 Intake @14.6psia, vacuum from 9.1 to 12.1psi @21:00:29.48 SS.reconfigure :inhaling 2.20ml/s @21:00:29.53 SS.seek 7ml @21:00:36.19 Stroke completed -- waiting for pressure to equalize ... @21:00:36.20 SS.reconfigure :exhale @21:00:36.25 SS.hold @21:00:46.20 Intake.select :close @21:00:46.36 SSV.dial :exhaust @21:00:47.12 Exhaust.select :open @21:00:47.23 SS.seek 0ml @21:00:51.28 Exhaust.select :close @21:00:51.39 Sampling 1000.0ml @21:00:51.40 Intake.select :close @21:00:51.41 Exhaust.select :close @21:00:51.43 SS.reconfigure :default @21:00:51.47 SS.hold @21:00:51.67 CTV.dial :intake @21:00:51.75 SSV.dial CBV @21:00:51.77
CBV.dial SSV @21:00:52.55 Allowing 130s for 25.0ml of remaining 1000.0ml @21:00:52.56 Intake.select :open @21:00:54.73 Intake @15.3psia, vacuum from 9.8 to 12.8psi @21:00:54.74 SS.reconfigure :inhaling 2.20ml/s @21:00:54.79 SS.seek 25ml @21:01:02.20 Vacuum too high -- waiting up to 40 seconds @21:01:02.22 SS.reconfigure :exhale @21:01:02.27 SS.hold @21:01:08.34 Intake @18.2psia, vacuum from 10.0 to 13.0psi @21:01:08.36 SS.reconfigure :inhaling 1.76ml/s @21:01:08.41 SS.seek 25ml @21:01:10.76 Vacuum too high -- waiting up to 40 seconds @21:01:10.78 SS.reconfigure :exhale @21:01:10.83 SS.hold @21:01:13.11 Intake @18.5psia, vacuum from 10.0 to 13.0psi @21:01:13.13 SS.reconfigure :inhaling 1.41ml/s @21:01:13.18 SS.seek 25ml @21:01:15.95 Vacuum too high -- waiting up to 40 seconds @21:01:15.96 SS.reconfigure :exhale @21:01:16.02 SS.hold @21:01:17.86 SS.reconfigure :inhaling 1.13ml/s @21:01:17.91 SS.seek 25ml @21:01:20.76 Vacuum too high -- waiting up to 40 seconds @21:01:20.78 SS.reconfigure :exhale @21:01:20.83 SS.hold @21:01:22.61 Intake @18.4psia, vacuum from 10.0 to 13.0psi @21:01:22.63 SS.reconfigure :inhaling 0.91ml/s @21:01:22.68 SS.seek 25ml @21:01:26.61 Vacuum too high -- waiting up to 40 seconds @21:01:26.62 SS.reconfigure :exhale @21:01:26.67 SS.hold @21:01:28.94 Intake @18.6psia, vacuum from 10.0 to 13.0psi @21:01:28.95 SS.reconfigure :inhaling 0.73ml/s @21:01:29.00 SS.seek 25ml @21:01:34.50 Stroke completed -- waiting for pressure to equalize ... @21:01:34.51 SS.reconfigure :exhale @21:01:34.56 SS.hold @21:01:59.05 Intake.select :close @21:01:59.21 SSV.dial :exhaust @21:01:59.97 Exhaust.select :open @21:02:00.09 SS.seek 0ml @21:02:09.18 Exhaust.select :close @21:02:09.29 SSV.dial CBV @21:02:09.33
CBV.dial SSV @21:02:10.07 Allowing 130s for 25.0ml of remaining 975.0ml @21:02:10.08 Intake.select :open @21:02:12.25 Intake @15.8psia, vacuum from 10.0 to 13.0psi @21:02:12.26 SS.reconfigure :inhaling 0.73ml/s @21:02:12.31 SS.seek 25ml @21:02:48.66 Stroke completed -- waiting for pressure to equalize ... @21:02:48.67 SS.reconfigure :exhale @21:02:49.14 SS.hold @21:03:19.78 Intake.select :close @21:03:19.94 SSV.dial :exhaust @21:03:20.71 Exhaust.select :open @21:03:20.82 SS.seek 0ml @21:03:29.63 Exhaust.select :close @21:03:29.74 SSV.dial CBV @21:03:29.76
CBV.dial SSV @21:03:30.53 Allowing 130s for 25.0ml of remaining 950.0ml @21:03:30.55 Intake.select :open @21:03:33.07 Intake @15.8psia, vacuum from 10.0 to 13.0psi @21:03:33.15 SS.reconfigure :inhaling 0.73ml/s @21:03:33.20 SS.seek 25ml @21:04:09.67 Stroke completed -- waiting for pressure to equalize ... @21:04:09.69 SS.reconfigure :exhale @21:04:09.73 SS.hold @21:04:36.18 Intake.select :close @21:04:36.33 SSV.dial :exhaust @21:04:37.10 Exhaust.select :open @21:04:37.22 SS.seek 0ml @21:04:46.03 Exhaust.select :close @21:04:46.14 SSV.dial CBV @21:04:46.16
CBV.dial SSV @21:04:46.94 Allowing 130s for 25.0ml of remaining 925.0ml @21:04:46.95 Intake.select :open @21:04:49.12 Intake @15.9psia, vacuum from 10.0 to 13.0psi @21:04:49.13 SS.reconfigure :inhaling 0.73ml/s @21:04:49.61 SS.seek 25ml @21:05:26.05 Stroke completed -- waiting for pressure to equalize ... @21:05:26.06 SS.reconfigure :exhale @21:05:26.10 SS.hold @21:05:48.55 Intake.select :close @21:05:48.70 SSV.dial :exhaust @21:05:49.47 Exhaust.select :open @21:05:49.58 SS.seek 0ml @21:05:58.56 Exhaust.select :close @21:05:58.68 SSV.dial CBV @21:05:58.72
CBV.dial SSV @21:05:59.47 Allowing 130s for 25.0ml of remaining 900.0ml @21:05:59.48 Intake.select :open @21:06:01.65 Intake @15.8psia, vacuum from 10.0 to 13.0psi @21:06:01.66 SS.reconfigure :inhaling 0.73ml/s @21:06:01.71 SS.seek 25ml @21:06:37.75 Stroke completed -- waiting for pressure to equalize ... @21:06:37.76 SS.reconfigure :exhale @21:06:37.81 SS.hold @21:06:58.48 Intake.select :close @21:06:58.64 SSV.dial :exhaust @21:06:59.41 Exhaust.select :open @21:06:59.53 SS.seek 0ml @21:07:08.34 Exhaust.select :close @21:07:08.45 SSV.dial CBV @21:07:08.47
CBV.dial SSV @21:07:09.26 Allowing 130s for 25.0ml of remaining 875.0ml @21:07:09.28 Intake.select :open @21:07:11.44 Intake @16.0psia, vacuum from 10.0 to 13.0psi @21:07:11.46 SS.reconfigure :inhaling 0.73ml/s @21:07:11.51 SS.seek 25ml @21:07:47.90 Stroke completed -- waiting for pressure to equalize ... @21:07:47.91 SS.reconfigure :exhale @21:07:47.96 SS.hold @21:08:06.92 Intake.select :close @21:08:07.08 SSV.dial :exhaust @21:08:07.84 Exhaust.select :open @21:08:07.96 SS.seek 0ml @21:08:16.72 Exhaust.select :close @21:08:16.83 SSV.dial CBV @21:08:16.86
CBV.dial SSV @21:08:17.61 Allowing 130s for 25.0ml of remaining 850.0ml @21:08:17.62 Intake.select :open @21:08:19.78 Intake @16.0psia, vacuum from 10.0 to 13.0psi @21:08:19.80 SS.reconfigure :inhaling 0.73ml/s @21:08:19.84 SS.seek 25ml @21:08:56.17 Stroke completed -- waiting for pressure to equalize ... @21:08:56.18 SS.reconfigure :exhale @21:08:56.22 SS.hold @21:09:12.54 Intake.select :close @21:09:12.70 SSV.dial :exhaust @21:09:13.45 Exhaust.select :open @21:09:13.56 SS.seek 0ml @21:09:22.31 Exhaust.select :close @21:09:22.42 SSV.dial CBV @21:09:22.44
CBV.dial SSV @21:09:23.23 Allowing 130s for 25.0ml of remaining 825.0ml @21:09:23.24 Intake.select :open @21:09:25.41 Intake @15.9psia, vacuum from 10.0 to 13.0psi @21:09:25.42 SS.reconfigure :inhaling 0.73ml/s @21:09:25.47 SS.seek 25ml @21:10:01.73 Stroke completed -- waiting for pressure to equalize ... @21:10:01.74 SS.reconfigure :exhale @21:10:01.79 SS.hold @21:10:17.98 Intake.select :close @21:10:18.14 SSV.dial :exhaust @21:10:18.90 Exhaust.select :open @21:10:19.02 SS.seek 0ml @21:10:27.74 Exhaust.select :close @21:10:27.85 SSV.dial CBV @21:10:27.88
CBV.dial SSV @21:10:28.65 Allowing 130s for 25.0ml of remaining 800.0ml @21:10:28.66 Intake.select :open @21:10:30.83 Intake @16.0psia, vacuum from 10.0 to 13.0psi @21:10:30.84 SS.reconfigure :inhaling 0.73ml/s @21:10:30.90 SS.seek 25ml @21:11:07.09 Stroke completed -- waiting for pressure to equalize ... @21:11:07.10 SS.reconfigure :exhale @21:11:07.14 SS.hold @21:11:23.48 Intake.select :close @21:11:24.06 SSV.dial :exhaust @21:11:24.82 Exhaust.select :open @21:11:24.94 SS.seek 0ml @21:11:33.85 Exhaust.select :close @21:11:33.96 SSV.dial CBV @21:11:33.99
CBV.dial SSV @21:11:34.76 Allowing 130s for 25.0ml of remaining 775.0ml @21:11:34.77 Intake.select :open @21:11:36.94 Intake @16.0psia, vacuum from 10.0 to 13.0psi @21:11:36.96 SS.reconfigure :inhaling 0.73ml/s @21:11:37.00 SS.seek 25ml @21:12:13.12 Stroke completed -- waiting for pressure to equalize ... @21:12:13.13 SS.reconfigure :exhale @21:12:13.17 SS.hold @21:12:31.52 Intake.select :close @21:12:31.68 SSV.dial :exhaust @21:12:32.46 Exhaust.select :open @21:12:32.58 SS.seek 0ml @21:12:41.55 Exhaust.select :close @21:12:41.67 SSV.dial CBV @21:12:41.69
CBV.dial SSV @21:12:42.48 Allowing 130s for 25.0ml of remaining 750.0ml @21:12:42.49 Intake.select :open @21:12:45.02 Intake @15.9psia, vacuum from 10.0 to 13.0psi @21:12:45.04 SS.reconfigure :inhaling 0.73ml/s @21:12:45.09 SS.seek 25ml @21:13:21.04 Stroke completed -- waiting for pressure to equalize ... @21:13:21.05 SS.reconfigure :exhale @21:13:21.10 SS.hold @21:13:39.36 Intake.select :close @21:13:39.52 SSV.dial :exhaust @21:13:40.27 Exhaust.select :open @21:13:40.39 SS.seek 0ml @21:13:49.16 Exhaust.select :close @21:13:49.27 SSV.dial CBV @21:13:49.29
CBV.dial SSV @21:13:50.07 Allowing 130s for 25.0ml of remaining 725.0ml @21:13:50.08 Intake.select :open @21:13:52.25 Intake @15.9psia, vacuum from 10.0 to 13.0psi @21:13:52.27 SS.reconfigure :inhaling 0.73ml/s @21:13:52.31 SS.seek 25ml @21:14:28.61 Stroke completed -- waiting for pressure to equalize ... @21:14:28.62 SS.reconfigure :exhale @21:14:28.67 SS.hold @21:14:49.32 Intake.select :close @21:14:49.47 SSV.dial :exhaust @21:14:50.23 Exhaust.select :open @21:14:50.35 SS.seek 0ml @21:14:59.30 Exhaust.select :close @21:14:59.41 SSV.dial CBV @21:14:59.43
CBV.dial SSV @21:15:00.24 Allowing 130s for 25.0ml of remaining 700.0ml @21:15:00.25 Intake.select :open @21:15:02.42 Intake @15.9psia, vacuum from 10.0 to 13.0psi @21:15:02.44 SS.reconfigure :inhaling 0.73ml/s @21:15:02.48 SS.seek 25ml @21:15:38.28 Stroke completed -- waiting for pressure to equalize ... @21:15:38.29 SS.reconfigure :exhale @21:15:38.34 SS.hold @21:15:59.17 Intake.select :close @21:15:59.32 SSV.dial :exhaust @21:16:00.10 Exhaust.select :open @21:16:00.22 SS.seek 0ml @21:16:09.00 Exhaust.select :close @21:16:09.11 SSV.dial CBV @21:16:09.14
CBV.dial SSV @21:16:09.91 Allowing 130s for 25.0ml of remaining 675.0ml @21:16:09.92 Intake.select :open @21:16:12.09 Intake @16.0psia, vacuum from 10.0 to 13.0psi @21:16:12.10 SS.reconfigure :inhaling 0.73ml/s @21:16:12.15 SS.seek 25ml @21:16:47.68 Vacuum too high -- waiting up to 40 seconds @21:16:47.70 SS.reconfigure :exhale @21:16:47.75 SS.hold @21:16:55.36 Intake @16.1psia, vacuum from 10.0 to 13.0psi @21:16:55.38 SS.reconfigure :inhaling 0.59ml/s @21:16:55.43 SS.seek 25ml @21:16:56.80 Stroke completed -- waiting for pressure to equalize ... @21:16:56.81 SS.reconfigure :exhale @21:16:56.86 SS.hold @21:17:11.16 Intake.select :close @21:17:11.32 SSV.dial :exhaust @21:17:12.08 Exhaust.select :open @21:17:12.20 SS.seek 0ml @21:17:21.17 Exhaust.select :close @21:17:21.28 SSV.dial CBV @21:17:21.31
CBV.dial SSV @21:17:22.08 Allowing 130s for 25.0ml of remaining 650.0ml @21:17:22.09 Intake.select :open @21:17:24.26 Intake @15.9psia, vacuum from 10.0 to 13.0psi @21:17:24.27 SS.reconfigure :inhaling 0.59ml/s @21:17:24.32 SS.seek 25ml @21:18:08.95 Stroke completed -- waiting for pressure to equalize ... @21:18:08.96 SS.reconfigure :exhale @21:18:09.00 SS.hold @21:18:27.28 Intake.select :close @21:18:27.43 SSV.dial :exhaust @21:18:28.20 Exhaust.select :open @21:18:28.32 SS.seek 0ml @21:18:37.27 Exhaust.select :close @21:18:37.37 SSV.dial CBV @21:18:37.39
CBV.dial SSV @21:18:38.20 Allowing 130s for 25.0ml of remaining 625.0ml @21:18:38.22 Intake.select :open @21:18:40.38 Intake @16.0psia, vacuum from 10.0 to 13.0psi @21:18:40.39 SS.reconfigure :inhaling 0.59ml/s @21:18:40.44 SS.seek 25ml @21:19:24.75 Stroke completed -- waiting for pressure to equalize ... @21:19:24.76 SS.reconfigure :exhale @21:19:24.80 SS.hold @21:19:45.58 Intake.select :close @21:19:45.73 SSV.dial :exhaust @21:19:46.51 Exhaust.select :open @21:19:46.62 SS.seek 0ml @21:19:55.37 Exhaust.select :close @21:19:55.48 SSV.dial CBV @21:19:55.51
CBV.dial SSV @21:19:56.28 Allowing 130s for 25.0ml of remaining 600.0ml @21:19:56.29 Intake.select :open @21:19:58.46 Intake @16.0psia, vacuum from 10.0 to 13.0psi @21:19:58.47 SS.reconfigure :inhaling 0.59ml/s @21:19:58.52 SS.seek 25ml @21:20:43.39 Stroke completed -- waiting for pressure to equalize ... @21:20:43.40 SS.reconfigure :exhale @21:20:43.44 SS.hold @21:21:05.91 Intake.select :close @21:21:06.07 SSV.dial :exhaust @21:21:06.83 Exhaust.select :open @21:21:06.94 SS.seek 0ml @21:21:15.75 Exhaust.select :close @21:21:15.86 SSV.dial CBV @21:21:15.88
CBV.dial SSV @21:21:16.65 Allowing 130s for 25.0ml of remaining 575.0ml @21:21:16.67 Intake.select :open @21:21:18.83 Intake @16.0psia, vacuum from 10.0 to 13.0psi @21:21:18.85 SS.reconfigure :inhaling 0.59ml/s @21:21:18.89 SS.seek 25ml @21:22:03.71 Stroke completed -- waiting for pressure to equalize ... @21:22:03.72 SS.reconfigure :exhale @21:22:03.77 SS.hold @21:22:28.67 Intake.select :close @21:22:28.82 SSV.dial :exhaust @21:22:29.59 Exhaust.select :open @21:22:29.71 SS.seek 0ml @21:22:38.39 Exhaust.select :close @21:22:38.49 SSV.dial CBV @21:22:38.51
CBV.dial SSV @21:22:39.31 Allowing 130s for 25.0ml of remaining 550.0ml @21:22:39.32 Intake.select :open @21:22:41.49 Intake @16.0psia, vacuum from 10.0 to 13.0psi @21:22:41.51 SS.reconfigure :inhaling 0.59ml/s @21:22:41.55 SS.seek 25ml @21:23:21.96 Vacuum too high -- waiting up to 40 seconds @21:23:21.97 SS.reconfigure :exhale @21:23:22.02 SS.hold @21:23:33.29 Intake @17.2psia, vacuum from 10.0 to 13.0psi @21:23:33.31 SS.reconfigure :inhaling 0.47ml/s @21:23:33.36 SS.seek 25ml @21:23:39.16 Stroke completed -- waiting for pressure to equalize ... @21:23:39.18 SS.reconfigure :exhale @21:23:39.22 SS.hold @21:23:55.53 Intake.select :close @21:23:55.68 SSV.dial :exhaust @21:23:56.82 Exhaust.select :open @21:23:57.01 SS.seek 0ml @21:24:05.81 Exhaust.select :close @21:24:05.91 SSV.dial CBV @21:24:05.94
CBV.dial SSV @21:24:06.73 Allowing 130s for 25.0ml of remaining 525.0ml @21:24:06.74 Intake.select :open @21:24:08.91 Intake @15.9psia, vacuum from 10.0 to 13.0psi @21:24:08.92 SS.reconfigure :inhaling 0.47ml/s @21:24:08.97 SS.seek 25ml @21:25:04.44 Stroke completed -- waiting for pressure to equalize ... @21:25:04.46 SS.reconfigure :exhale @21:25:04.50 SS.hold @21:25:27.03 Intake.select :close @21:25:27.18 SSV.dial :exhaust @21:25:27.95 Exhaust.select :open @21:25:28.06 SS.seek 0ml @21:25:37.11 Exhaust.select :close @21:25:37.22 SSV.dial CBV @21:25:37.24
CBV.dial SSV @21:25:38.02 Allowing 130s for 25.0ml of remaining 500.0ml @21:25:38.04 Intake.select :open @21:25:40.20 Intake @16.0psia, vacuum from 10.0 to 13.0psi @21:25:40.22 SS.reconfigure :inhaling 0.47ml/s @21:25:40.26 SS.seek 25ml @21:26:35.41 Stroke completed -- waiting for pressure to equalize ... @21:26:35.42 SS.reconfigure :exhale @21:26:35.47 SS.hold @21:26:59.96 Intake.select :close @21:27:00.11 SSV.dial :exhaust @21:27:00.90 Exhaust.select :open @21:27:01.01 SS.seek 0ml @21:27:09.97 Exhaust.select :close @21:27:10.50 SSV.dial CBV @21:27:10.53
CBV.dial SSV @21:27:11.32 Allowing 130s for 25.0ml of remaining 475.0ml @21:27:11.33 Intake.select :open @21:27:13.50 Intake @16.0psia, vacuum from 10.0 to 13.0psi @21:27:13.52 SS.reconfigure :inhaling 0.47ml/s @21:27:13.57 SS.seek 25ml @21:28:08.66 Stroke completed -- waiting for pressure to equalize ... @21:28:08.67 SS.reconfigure :exhale @21:28:08.72 SS.hold @21:28:35.26 Intake.select :close @21:28:35.41 SSV.dial :exhaust @21:28:36.20 Exhaust.select :open @21:28:36.32 SS.seek 0ml @21:28:45.57 Exhaust.select :close @21:28:45.68 SSV.dial CBV @21:28:45.72
CBV.dial SSV @21:28:46.49 Allowing 130s for 25.0ml of remaining 450.0ml @21:28:46.50 Intake.select :open @21:28:48.67 Intake @16.1psia, vacuum from 10.0 to 13.0psi @21:28:48.69 SS.reconfigure :inhaling 0.47ml/s @21:28:48.74 SS.seek 25ml @21:29:36.73 Vacuum too high -- waiting up to 40 seconds @21:29:36.74 SS.reconfigure :exhale @21:29:36.80 SS.hold @21:29:49.61 Intake @17.9psia, vacuum from 10.0 to 13.0psi @21:29:49.63 SS.reconfigure :inhaling 0.38ml/s @21:29:49.68 SS.seek 25ml @21:29:59.88 Stroke completed -- waiting for pressure to equalize ... @21:29:59.89 SS.reconfigure :exhale @21:29:59.93 SS.hold @21:30:18.41 Intake.select :close @21:30:18.57 SSV.dial :exhaust @21:30:19.33 Exhaust.select :open @21:30:19.45 SS.seek 0ml @21:30:28.30 Exhaust.select :close @21:30:28.41 SSV.dial CBV @21:30:28.43
CBV.dial SSV @21:30:29.22 Allowing 130s for 25.0ml of remaining 425.0ml @21:30:29.23 Intake.select :open @21:30:31.40 Intake @16.1psia, vacuum from 10.0 to 13.0psi @21:30:31.41 SS.reconfigure :inhaling 0.38ml/s @21:30:31.46 SS.seek 25ml @21:31:39.94 Stroke completed -- waiting for pressure to equalize ... @21:31:39.95 SS.reconfigure :exhale @21:31:39.99 SS.hold @21:32:04.84 Intake.select :close @21:32:04.99 SSV.dial :exhaust @21:32:05.75 Exhaust.select :open @21:32:05.87 SS.seek 0ml @21:32:14.67 Exhaust.select :close @21:32:14.78 SSV.dial CBV @21:32:14.80
CBV.dial SSV @21:32:15.59 Allowing 130s for 25.0ml of remaining 400.0ml @21:32:15.61 Intake.select :open @21:32:17.77 Intake @16.1psia, vacuum from 10.0 to 13.0psi @21:32:17.79 SS.reconfigure :inhaling 0.38ml/s @21:32:17.83 SS.seek 25ml @21:33:26.65 Stroke completed -- waiting for pressure to equalize ... @21:33:26.66 SS.reconfigure :exhale @21:33:26.71 SS.hold @21:33:53.24 Intake.select :close @21:33:53.40 SSV.dial :exhaust @21:33:54.18 Exhaust.select :open @21:33:54.30 SS.seek 0ml @21:34:03.31 Exhaust.select :close @21:34:03.42 SSV.dial CBV @21:34:03.44
CBV.dial SSV @21:34:04.25 Allowing 130s for 25.0ml of remaining 375.0ml @21:34:04.26 Intake.select :open @21:34:06.43 Intake @16.1psia, vacuum from 10.0 to 13.0psi @21:34:06.44 SS.reconfigure :inhaling 0.38ml/s @21:34:06.49 SS.seek 25ml @21:35:14.57 Stroke completed -- waiting for pressure to equalize ... @21:35:14.58 SS.reconfigure :exhale @21:35:14.63 SS.hold @21:35:49.36 Intake.select :close @21:35:49.52 SSV.dial :exhaust @21:35:50.28 Exhaust.select :open @21:35:50.39 SS.seek 0ml @21:35:59.25 Exhaust.select :close @21:35:59.35 SSV.dial CBV @21:35:59.37
CBV.dial SSV @21:36:00.17 Allowing 130s for 25.0ml of remaining 350.0ml @21:36:00.18 Intake.select :open @21:36:02.35 Intake @16.2psia, vacuum from 10.0 to 13.0psi @21:36:02.36 SS.reconfigure :inhaling 0.38ml/s @21:36:02.41 SS.seek 25ml @21:37:11.01 Stroke completed -- waiting for pressure to equalize ... @21:37:11.02 SS.reconfigure :exhale @21:37:11.07 SS.hold @21:37:49.97 Intake.select :close @21:37:50.12 SSV.dial :exhaust @21:37:50.90 Exhaust.select :open @21:37:51.02 SS.seek 0ml @21:37:59.85 Exhaust.select :close @21:37:59.96 SSV.dial CBV @21:37:59.98
CBV.dial SSV @21:38:00.76 Allowing 130s for 25.0ml of remaining 325.0ml @21:38:00.77 Intake.select :open @21:38:02.94 Intake @16.2psia, vacuum from 10.0 to 13.0psi @21:38:03.41 SS.reconfigure :inhaling 0.38ml/s @21:38:03.53 SS.seek 25ml @21:39:08.41 Vacuum too high -- waiting up to 40 seconds @21:39:08.42 SS.reconfigure :exhale @21:39:08.47 SS.hold @21:39:26.98 Intake @17.2psia, vacuum from 10.0 to 13.0psi @21:39:27.00 SS.reconfigure :inhaling 0.31ml/s @21:39:27.05 SS.seek 25ml @21:39:31.93 Stroke completed -- waiting for pressure to equalize ... @21:39:31.94 SS.reconfigure :exhale @21:39:31.98 SS.hold @21:39:56.43 Intake.select :close @21:39:56.58 SSV.dial :exhaust @21:39:57.35 Exhaust.select :open @21:39:57.47 SS.seek 0ml @21:40:06.51 Exhaust.select :close @21:40:06.61 SSV.dial CBV @21:40:06.63
CBV.dial SSV @21:40:07.43 Allowing 130s for 25.0ml of remaining 300.0ml @21:40:07.44 Intake.select :open @21:40:09.61 Intake @16.2psia, vacuum from 10.0 to 13.0psi @21:40:09.63 SS.reconfigure :inhaling 0.31ml/s @21:40:09.67 SS.seek 25ml @21:41:36.86 Stroke completed -- waiting for pressure to equalize ... @21:41:36.87 SS.reconfigure :exhale @21:41:36.92 SS.hold @21:42:13.74 Intake.select :close @21:42:13.90 SSV.dial :exhaust @21:42:14.71 Exhaust.select :open @21:42:14.82 SS.seek 0ml @21:42:23.64 Exhaust.select :close @21:42:23.75 SSV.dial CBV @21:42:23.77
CBV.dial SSV @21:42:24.57 Allowing 130s for 25.0ml of remaining 275.0ml @21:42:24.58 Intake.select :open @21:42:26.74 Intake @16.3psia, vacuum from 10.0 to 13.0psi @21:42:26.75 SS.reconfigure :inhaling 0.31ml/s @21:42:26.80 SS.seek 25ml @21:43:54.34 Stroke completed -- waiting for pressure to equalize ... @21:43:54.35 SS.reconfigure :exhale @21:43:54.40 SS.hold @21:44:34.58 Intake.select :close @21:44:34.73 SSV.dial :exhaust @21:44:35.50 Exhaust.select :open @21:44:35.62 SS.seek 0ml @21:44:45.12 Exhaust.select :close @21:44:45.23 SSV.dial CBV @21:44:45.25
CBV.dial SSV @21:44:46.06 Allowing 130s for 25.0ml of remaining 250.0ml @21:44:46.08 Intake.select :open @21:44:48.25 Intake @16.2psia, vacuum from 10.0 to 13.0psi @21:44:48.27 SS.reconfigure :inhaling 0.31ml/s @21:44:48.31 SS.seek 25ml @21:46:15.43 Stroke completed -- waiting for pressure to equalize ... @21:46:15.45 SS.reconfigure :exhale @21:46:15.49 SS.hold @21:46:55.67 SS.reconfigure :default @21:46:55.72 SS.hold @21:46:55.79 Intake.select :close @21:46:55.94 ShallowSampler::Clogged in MAIN -- Intake @16psia & Outlet @14psia won't equalize after 775.0ml @21:46:55.95 SSV.dial :exhaust @21:46:56.73 Exhaust.select :open @21:46:56.85 SS.reconfigure :exhale @21:46:56.90 SS.seek 0ml @21:47:05.94 Exhaust.select :close @21:47:06.04 Sampled 775.0ml @21:47:06.06 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) Intake.select :close @21:47:06.13 CSV.dial :waste @21:47:06.26 SSV.dial CBV,avoiding: :exhaust @21:47:06.28
CBV.dial SSV @21:47:06.43 CS.seek 0ml @21:47:06.50 CSV.dial :air @21:47:07.24 CS.seek 10ml @21:47:08.20
CTV.dial :air @21:47:08.98 delay 15 seconds @21:47:24.00 SSV.dial :spit,avoiding: :exhaust @21:47:25.32 CTV.dial CSV,avoiding: :intake @21:47:25.36
SS.seek 0ml @21:47:25.48 SSV.dial CBV,avoiding: :exhaust @21:47:31.72 SSV.dial between CBV and plug @21:47:32.21 CBV.dial between SSV and waste2 @21:47:32.59 CSV.dial between air and CTV @21:47:32.90 CS.seek 8.381ml @21:47:37.38 CTV.dial CSV,avoiding: :air @21:47:37.40
CSV.dial CTV @21:47:37.80 CS.reconfigure :pressurize @21:47:37.85 CS.seek 0ml @21:47:53.22 BubblePoint: Intake @24.2psia, Outlet @0.9psia @21:47:53.23 CBV.dial SSV @21:47:53.58 SS.reconfigure :default @21:47:53.63 SS.seek 10ml @21:47:59.15 delay 15 seconds @21:48:14.28 Evacuated: Intake @23.7psia, Outlet @23.3psia @21:48:14.29 SSV.dial :spit,avoiding: :exhaust @21:48:15.75 SS.reconfigure :exhale @21:48:15.80 SS.seek 0ml @21:48:20.84 SS.reconfigure :fast @21:48:20.88 SS.seek 5ml @21:48:23.66 SSV.dial CBV,avoiding: :exhaust @21:48:23.68
CBV.dial SSV @21:48:25.03 CTV.dial :air,avoiding: :intake @21:48:26.34 CSV.dial CTV @21:48:26.38
SS.seek 10ml @21:48:27.60 CSV.dial :air @21:48:28.03 CS.reconfigure :fast @21:48:28.08 CS.seek 0ml @21:48:28.92
SSV.dial :spit,avoiding: :exhaust @21:48:30.29 SS.reconfigure :exhale @21:48:30.34 SS.seek 0ml @21:48:34.74 CS.stop @21:48:35.32
SS.reconfigure :fast @21:48:35.37 SS.seek 5ml @21:48:38.21 SSV.dial CBV,avoiding: :exhaust @21:48:38.23
CBV.dial SSV @21:48:40.57 SSV.dial :spit,avoiding: :exhaust @21:48:41.88 SS.reconfigure :default @21:48:41.93 SS.seek 0ml @21:48:45.15 SS.stop @21:48:45.19 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @21:48:45.20 Intake.select :close @21:48:45.27 CSV.dial :waste @21:48:45.40 SSV.dial CBV,avoiding: :exhaust @21:48:45.42
CBV.dial SSV @21:48:46.06 CS.seek 0ml @21:48:46.13 CSV.dial :air @21:48:46.75
CTV.dial :air @21:48:46.86 CS.seek 10ml @21:48:46.93
delay 15 seconds @21:49:01.95 SSV.dial :spit,avoiding: :exhaust @21:49:03.29 CTV.dial CSV,avoiding: :intake @21:49:03.31
SS.reconfigure :exhale @21:49:03.42 SS.seek 0ml @21:49:03.78 SSV.dial CBV,avoiding: :exhaust @21:49:11.22 SSV.dial between CBV and plug @21:49:11.70 CBV.dial between SSV and waste2 @21:49:12.10 CSV.dial between air and CTV @21:49:12.43 CS.seek 7.475ml @21:49:18.94 CTV.dial CSV,avoiding: :air @21:49:18.96
CSV.dial CTV @21:49:19.68 CS.reconfigure :pressurize @21:49:19.73 CS.seek 0ml @21:49:32.63 BubblePoint: Intake @24.3psia, Outlet @3.5psia @21:49:32.64 CBV.dial SSV @21:49:32.98 SS.reconfigure :default @21:49:33.03 SS.seek 10ml @21:49:38.60 delay 15 seconds @21:49:53.74 Evacuated: Intake @23.9psia, Outlet @23.7psia @21:49:53.75 SSV.dial :spit,avoiding: :exhaust @21:49:54.78 SS.reconfigure :exhale @21:49:54.83 SS.seek 0ml @21:49:59.85 SS.reconfigure :fast @21:49:59.90 SS.seek 5ml @21:50:02.74 SSV.dial CBV,avoiding: :exhaust @21:50:02.78
CBV.dial SSV @21:50:04.08 CTV.dial :air,avoiding: :intake @21:50:05.37 CSV.dial CTV @21:50:05.41
SS.seek 10ml @21:50:06.61 CSV.dial :air @21:50:07.06 CS.reconfigure :fast @21:50:07.11 CS.seek 0ml @21:50:07.92
SSV.dial :spit,avoiding: :exhaust @21:50:09.25 SS.reconfigure :exhale @21:50:09.30 SS.seek 0ml @21:50:14.05 CS.stop @21:50:14.29
SS.reconfigure :fast @21:50:14.33 SS.seek 5ml @21:50:17.14 SSV.dial CBV,avoiding: :exhaust @21:50:17.16
CBV.dial SSV @21:50:19.52 SSV.dial :spit,avoiding: :exhaust @21:50:20.83 SS.reconfigure :default @21:50:20.88 SS.seek 0ml @21:50:24.15 SS.stop @21:50:24.19 Lysing: Adding 2.0ml AqMeOH @21:50:24.20 CBV.dial between CSV and waste1,avoiding: :DBV @21:50:25.70 CTV.dial CSV @21:50:27.01 CSV.dial CSR @21:50:27.80 Pulling 1ml of CSR.AqMeOH @21:50:27.81 CSR.select :AqMeOH @21:50:27.93 CS.reconfigure :default @21:50:27.98 CS.seek 1ml @21:50:31.37 delay 5 seconds @21:50:36.39 CSR.select :air @21:50:36.56 CS.seek 6ml @21:50:50.21 delay 2 seconds @21:50:52.23 CSV.dial :waste2 @21:50:52.80 CS.reconfigure :fast @21:50:52.84 CS.seek 0ml @21:51:07.59 CSV.dial CSR @21:51:08.03 Pulling 2ml of CSR.AqMeOH CSR.select :AqMeOH @21:51:08.15 CS.reconfigure :default @21:51:08.19 CS.seek 2ml @21:51:14.21 delay 5 seconds @21:51:19.23 CSR.select :air @21:51:19.40 CS.seek 5.001ml @21:51:27.90 delay 2 seconds @21:51:29.92 CSV.dial CTV @21:51:30.36 CS.seek 1ml @21:51:41.60 Before lysing Collection Puck Heater at 13.8C/31.8psia @21:51:41.62 Heating Puck.from 5, :sh1 in Collection Clamp to 70C for 10:00 @21:51:41.64 CH.seek 70C,8:00 @21:53:50.81 CH.hold 10:00,70C @21:53:51.00 Begun lysing Collection Puck Heater at 68.1C/38.4psia @22:03:51.01 Ended lysing Collection Puck Heater at 70C/37.5psia @22:03:51.03 CS.seek 0ml @22:03:54.55 CTV.dial between spare and air,avoiding: :intake @22:03:55.57 CSV.dial :air @22:03:56.03 CS.seek 5ml @22:04:09.82 CSV.dial CTV @22:04:10.28 delay 2 seconds @22:04:12.29 CSV.dial :air @22:04:12.75 delay 2 seconds @22:04:14.76 CSV.dial CTV @22:04:15.22 delay 2 seconds @22:04:17.23 CSV.dial :air @22:04:17.68 delay 2 seconds @22:04:19.71 CS.seek 0ml @22:04:33.35 Puck @84.6psia after final lysis pressurization @22:04:33.36 Recovering Lysate into Collection syringe @22:04:33.37 CSV.dial CBV @22:04:34.39 CBV.dial CSV @22:04:34.77 CS.seek 5ml @22:04:48.36 delay 5 seconds @22:04:53.38 CTV.dial :air @22:04:53.91 CS.seek 7ml @22:05:00.07 delay 5 seconds @22:05:05.09 CSV.dial :air2 @22:05:05.55 CS.seek 10ml @22:05:13.98 CSV.dial between air and CTV,avoiding: CBV @22:05:15.14 Lysing Complete @22:05:15.21 Storing sh1 puck in Collection Clamp to tube 4 @22:05:15.83 SC.reconfigure :tight @22:05:15.88 SC.seek 4 @22:05:19.46 SC.configure :limp @22:05:19.68 Elbow.reconfigure :tight @22:05:19.73 Elbow.seek 4 @22:05:21.46 Forearm.reconfigure :tight @22:05:21.50 Forearm.seek 4 + 450 counts @22:05:22.57 SE.reconfigure :pick @22:05:22.63 SE.seek :beamBreak @22:05:25.66 SE.configure :default @22:05:25.82 Elbow.reconfigure :limp @22:05:25.87 Forearm.reconfigure :loose @22:05:25.92 Forearm.seek 4 @22:05:26.15 Elbow.hold @22:05:26.21 Elbow.reconfigure :tight @22:05:26.31 SE.seek 0.16 Pucks below Top Plate @22:05:26.74 Forearm.force -20 @22:05:26.78 Forearm.seek 4 + 317 counts @22:05:27.06 Forearm.reconfigure :tight @22:05:27.11 Forearm.seek 4 + 450 counts @22:05:27.30 SE.seek 1.16 Pucks below Top Plate @22:05:27.79 Forearm.reconfigure :loose @22:05:27.84 Forearm.seek :retract @22:05:28.82 Elbow.seek CC @22:05:30.50 Elbow.reconfigure :loose @22:05:30.60 Forearm.seek CC @22:05:31.62 Hand.close @22:05:32.26 CC.seek :open @22:05:37.37 Forearm.seek :retract @22:05:38.42 Elbow.reconfigure :tight @22:05:38.47 Elbow.seek 4 @22:05:40.21 Elbow.reconfigure :limp @22:05:40.26 Forearm.seek 4 @22:05:41.27 Elbow.hold @22:05:41.34 Elbow.reconfigure :tight @22:05:41.39 Hand.open @22:05:42.13 Forearm.force -20 @22:05:42.17 Forearm.seek 4 + 316 counts @22:05:42.52 Forearm.reconfigure :tight @22:05:42.57 Forearm.seek 4 + 450 counts @22:05:42.84 SE.seek :down @22:05:43.97
Forearm.reconfigure :loose @22:05:44.01 Forearm.seek :retract @22:05:45.47 SE.stop @22:05:45.51
Tranferring DA @22:05:45.52 Beginning samplePassC @22:05:45.54 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @22:05:45.72 Elbow.seek FlushPuck::Garage @22:05:47.60 Elbow.reconfigure :loose @22:05:47.70 Forearm.seek :garage @22:05:48.66 Hand.close @22:05:49.38 Forearm.seek :retract @22:05:50.34 Elbow.reconfigure :tight @22:05:50.39 Elbow.seek CC @22:05:53.35 Elbow.reconfigure :loose @22:05:53.46 Forearm.reconfigure :tight @22:05:53.51 Forearm.seek CC @22:05:55.20 CC.seek :closed @22:05:59.73 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.40 @22:05:59.80 Hand.open @22:06:00.44 Forearm.reconfigure :loose @22:06:00.49 Forearm.seek :retract @22:06:01.44 PSV.dial PSR @22:06:02.84 Pulling 1.5ml of PSR.flush @22:06:02.85 PSR.select :flush @22:06:02.97 PS.seek 1.5ml @22:06:19.11 delay 5 seconds @22:06:24.13 PSV.dial :waste1 @22:06:24.60 PS.seek 0ml @22:06:40.70 PSV.dial PSR @22:06:41.17 PSR.select :air @22:06:41.29 PS.seek 2.5ml @22:07:07.70 PRV.dial CBV,avoiding: :ab2 @22:07:07.73
CBV.dial PRV @22:07:09.47 PSV.dial :mixloop @22:07:10.62 CTV.dial :air,avoiding: :intake @22:07:10.79 PTV.dial :PRVmixing @22:07:12.13 PS.seek 0ml @22:07:38.54 PSV.dial :air @22:07:39.03 PS.seek 2.5ml @22:08:05.42 PSV.dial :mixloop @22:08:05.91 PS.seek 0ml @22:08:32.20 PSV.dial :air @22:08:32.67 PS.seek 2.5ml @22:08:59.07 PSV.dial :mixloop @22:08:59.55 PS.seek 0ml @22:09:25.86 Garaging FlushPuck in Collection Clamp @22:09:25.92 Elbow.reconfigure :tight @22:09:26.35 Elbow.seek CC @22:09:26.71 Elbow.reconfigure :loose @22:09:26.81 Forearm.seek CC @22:09:27.86 Hand.close @22:09:28.51 CC.seek :open @22:09:33.12 Forearm.seek :retract @22:09:34.12 Elbow.reconfigure :tight @22:09:34.16 Elbow.seek :garage @22:09:37.12 Elbow.reconfigure :loose @22:09:37.22 Forearm.reconfigure :tight @22:09:37.27 Forearm.seek :garage @22:09:38.34 Hand.open @22:09:39.07 Forearm.reconfigure :loose @22:09:39.11 Forearm.seek :retract @22:09:40.08 PSV.dial :mixloop @22:09:40.17 PTV.dial :PRVmixing @22:09:40.25 PRV.dial CBV @22:09:40.27
CBV.dial PRV @22:09:40.49 CTV.dial :air,avoiding: :intake @22:09:40.51 Loading lyfil puck from tube 5 into Collection Clamp @22:09:40.75 SC.reconfigure :tight @22:09:40.79 SC.seek 5 @22:09:44.28 SC.configure :limp @22:09:44.50 Elbow.reconfigure :tight @22:09:44.54 Elbow.seek 5 @22:09:46.47 Forearm.reconfigure :tight @22:09:46.52 Forearm.seek 5 + 450 counts @22:09:47.55 SE.reconfigure :pick @22:09:47.61 SE.seek :beamBreak @22:09:48.72 SE.configure :default @22:09:49.33 Elbow.seek 5 @22:09:49.52 Forearm.seek 5 + 300 counts @22:09:49.80 Forearm.reconfigure :loose @22:09:49.85 Hand.close @22:09:50.52 SE.seek :down @22:09:50.71
Forearm.seek :retract @22:09:51.28 SE.stop @22:09:51.63
Elbow.seek CC @22:09:53.20 Elbow.reconfigure :loose @22:09:53.30 Forearm.reconfigure :tight @22:09:53.35 Forearm.seek CC @22:09:54.63 CC.seek :closed @22:09:59.14 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.16 @22:09:59.15 Hand.open @22:09:59.83 Forearm.reconfigure :loose @22:09:59.87 Forearm.seek :retract @22:10:00.85 CTV.dial CSV,avoiding: :intake @22:10:02.19 CSV.dial CTV @22:10:02.53 PS.seek 2.5ml @22:10:28.91 CS.seek 4.5ml @22:10:43.82 delay 8 seconds @22:10:51.84 PSV.dial :air @22:10:52.31 delay 2 seconds @22:10:54.32 PSV.dial :waste2 @22:10:55.06 PS.seek 0ml @22:11:21.44 CBV.dial between PRV and waste1 @22:11:22.22 CSV.dial :air @22:11:22.70 CS.seek 10ml @22:11:37.53 CSV.dial CTV @22:11:37.99 CS.seek 0ml @22:12:04.60 CBV.dial :waste1 @22:12:04.91 delay 5 seconds @22:12:09.93 CTV.dial :air,avoiding: :intake @22:12:11.33 Storing lyfil puck in Collection Clamp to tube 4 @22:12:11.51 SC.reconfigure :tight @22:12:11.56 SC.seek 4 @22:12:15.10 SC.configure :limp @22:12:15.32 Elbow.reconfigure :tight @22:12:15.36 Elbow.seek 4 @22:12:17.07 Forearm.reconfigure :tight @22:12:17.12 Forearm.seek 4 + 450 counts @22:12:18.27 SE.reconfigure :pick @22:12:18.33 SE.seek :beamBreak @22:12:21.36 SE.configure :default @22:12:21.52 Elbow.reconfigure :limp @22:12:21.57 Forearm.reconfigure :loose @22:12:21.62 Forearm.seek 4 @22:12:21.83 Elbow.hold @22:12:21.90 Elbow.reconfigure :tight @22:12:22.00 SE.seek 1.17 Pucks below Top Plate @22:12:22.55 Forearm.force -20 @22:12:22.59 Forearm.seek 4 + 304 counts @22:12:22.87 Forearm.reconfigure :tight @22:12:22.92 Forearm.seek 4 + 450 counts @22:12:23.12 SE.seek 2.17 Pucks below Top Plate @22:12:24.12 Forearm.reconfigure :loose @22:12:24.17 Forearm.seek :retract @22:12:25.10 Elbow.seek CC @22:12:26.80 Elbow.reconfigure :loose @22:12:26.90 Forearm.seek CC @22:12:27.85 Hand.close @22:12:28.51 CC.seek :open @22:12:33.24 Forearm.seek :retract @22:12:34.19 Elbow.reconfigure :tight @22:12:34.24 Elbow.seek 4 @22:12:36.00 Elbow.reconfigure :limp @22:12:36.05 Forearm.seek 4 @22:12:37.00 Elbow.hold @22:12:37.07 Elbow.reconfigure :tight @22:12:37.12 Hand.open @22:12:37.88 Forearm.force -20 @22:12:37.92 Forearm.seek 4 + 301 counts @22:12:38.32 Forearm.reconfigure :tight @22:12:38.37 Forearm.seek 4 + 450 counts @22:12:38.68 SE.seek :down @22:12:39.81
Forearm.reconfigure :loose @22:12:39.86 Forearm.seek :retract @22:12:41.21 SE.stop @22:12:41.26
PSV.dial :air @22:12:42.01 PS.seek 2.5ml @22:13:08.32 delay 1 second @22:13:09.33 PSV.dial :mixloop @22:13:09.79 delay 1 second @22:13:10.80 PSV.dial :air @22:13:11.64 delay 1 second @22:13:12.72 PSV.dial :mixloop @22:13:13.20 delay 1 second @22:13:14.21 PSV.dial :air @22:13:14.68 delay 1 second @22:13:15.70 PS.seek 0ml @22:13:42.10 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @22:13:42.29 Elbow.seek FlushPuck::Garage @22:13:44.03 Elbow.reconfigure :loose @22:13:44.13 Forearm.seek :garage @22:13:45.08 Hand.close @22:13:45.87 Forearm.seek :retract @22:13:46.88 Elbow.reconfigure :tight @22:13:46.93 Elbow.seek PC @22:13:48.18 Elbow.reconfigure :loose @22:13:48.28 Forearm.reconfigure :tight @22:13:48.33 Forearm.seek PC @22:13:49.62 PC.seek :closed @22:13:54.29 Processing Clamp onSpring/offSpring for 16/26 samples, on/off torque 1.62 @22:13:54.36 Hand.open @22:13:55.07 Forearm.reconfigure :loose @22:13:55.11 Forearm.seek :retract @22:13:56.06 PRV.dial :air @22:13:56.52 PSV.dial :mixloop @22:13:57.05 PS.seek 0.3ml @22:14:01.00 PSV.dial PSR @22:14:02.51 Pulling 0.4ml of PSR.air @22:14:02.52 PSR.select :air @22:14:02.54 PS.seek 0.7ml @22:14:07.51 delay 3 seconds @22:14:10.52 PSR.select :air @22:14:10.55 PS.seek 2.5ml @22:14:29.76 delay 3 seconds @22:14:32.77 PSV.dial :mixloop @22:14:33.83 PTV.dial :mixing @22:14:34.58 PBV.dial :waste2 @22:14:35.54 PS.seek 0ml @22:15:01.85 Garaging FlushPuck in Processing Clamp @22:15:01.92 Elbow.reconfigure :tight @22:15:01.97 Elbow.seek PC @22:15:02.09 Elbow.reconfigure :loose @22:15:02.19 Forearm.seek PC @22:15:03.14 Hand.close @22:15:03.76 PC.seek :open @22:15:08.50 Forearm.seek :retract @22:15:09.47 Elbow.reconfigure :tight @22:15:09.52 Elbow.seek :garage @22:15:10.89 Elbow.reconfigure :loose @22:15:10.99 Forearm.reconfigure :tight @22:15:11.03 Forearm.seek :garage @22:15:12.08 Hand.open @22:15:12.69 Forearm.reconfigure :loose @22:15:12.73 Forearm.seek :retract @22:15:13.75 Loading da puck from tube 5 into Processing Clamp @22:15:14.01 SC.reconfigure :tight @22:15:14.06 SC.seek 5 @22:15:17.62 SC.configure :limp @22:15:17.84 Elbow.reconfigure :tight @22:15:17.89 Elbow.seek 5 @22:15:19.76 Forearm.reconfigure :tight @22:15:19.81 Forearm.seek 5 + 450 counts @22:15:20.89 SE.reconfigure :pick @22:15:21.39 SE.seek :beamBreak @22:15:22.61 SE.configure :default @22:15:22.77 Elbow.seek 5 @22:15:23.15 Forearm.seek 5 + 300 counts @22:15:23.42 Forearm.reconfigure :loose @22:15:23.47 Hand.close @22:15:24.14 SE.seek :down @22:15:24.33
Forearm.seek :retract @22:15:24.96 SE.stop @22:15:25.36
Elbow.seek PC @22:15:27.86 Elbow.reconfigure :loose @22:15:27.96 Forearm.reconfigure :tight @22:15:28.01 Forearm.seek PC @22:15:29.23 PC.seek :closed @22:15:33.95 Processing Clamp onSpring/offSpring for 16/26 samples, on/off torque 1.65 @22:15:33.96 Hand.open @22:15:34.68 Forearm.reconfigure :loose @22:15:34.73 Forearm.seek :retract @22:15:35.70 Heating Puck.from 5, :da in Processing Clamp to 28C for 2:00:00 @22:15:35.72 PH.seek 28C,5:00 @22:16:15.97 PH.hold 2:00:00,28C @22:16:16.02 delay 1:30 @22:17:46.07 PSR.select :air @22:17:46.09 PSV.dial PSR @22:17:47.16 Pulling 0.5ml of PSR.wash @22:17:47.17 PSR.select :wash @22:17:47.29 PS.seek 0.5ml @22:17:53.19 delay 5 seconds @22:17:58.21 PSR.select :air @22:17:58.33 PS.seek 2ml @22:18:14.53 PTV.dial :puck @22:18:15.28 PSV.dial PTV @22:18:16.05 PBV.dial :waste1 @22:18:17.44 PS.seek 1.5ml @22:18:23.38 delay 3 seconds @22:18:26.39 PBV.dial between PSV and waste1 @22:18:26.76 PS.seek 0ml @22:18:42.94 delay 5:00 @22:23:42.96 recoverPSRreagent08 @22:23:42.97 PTV.dial :puck @22:23:43.15 PBV.dial :waste1 @22:23:43.53 delay 30 seconds @22:24:13.55 PBV.dial between PSV and waste1 @22:24:13.93 PSR.select :air @22:24:13.95 PSV.dial PSR @22:24:14.70 PS.reconfigure :fast @22:24:14.75 PS.seek 2.5ml @22:24:38.59 PSV.dial PTV @22:24:39.35 delay 5 seconds @22:24:44.37 PSV.dial PSR @22:24:45.14 delay 5 seconds @22:24:50.16 PSV.dial PTV @22:24:50.92 delay 5 seconds @22:24:55.94 PSV.dial :air @22:24:56.70 PS.seek 0ml @22:25:19.81 PTV.dial :PRVmixing,via: PRV @22:25:21.15 PSV.dial :mixloop @22:25:21.62 PRV.dial :ab1 @22:25:22.36 PS.reconfigure :slow @22:25:22.41 PS.seek 0.15ml @22:25:26.27 delay 2 seconds @22:25:28.29 CBV.dial PRV @22:25:28.32
PRV.dial CBV @22:25:28.88 PS.seek 0.35ml @22:25:33.49 delay 2 seconds @22:25:35.51 CBV.dial between PRV and PSV @22:25:35.82 PRV.dial :air @22:25:36.28 PS.seek 0.9ml @22:25:47.99 PRV.dial :waste2 @22:25:48.75 PS.seek 0.5ml @22:25:57.37 addPMLReagent08 @22:25:57.38 PSV.dial :air @22:25:57.84 PS.reconfigure :default @22:25:57.89 PS.seek 2ml @22:26:14.08 PSV.dial :PML @22:26:14.55 PTV.dial :mixing @22:26:15.30 PBV.dial :waste1 @22:26:15.74 PS.seek 1.85ml @22:26:18.21 PBV.dial between PSV and waste1 @22:26:18.58 PS.seek 0ml @22:26:38.37 delay 2 seconds @22:26:40.39 Beginning samplePassC @22:26:40.40 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @22:26:40.58 Elbow.reconfigure :tight @22:26:40.63 Elbow.seek FlushPuck::Garage @22:26:42.00 Elbow.reconfigure :loose @22:26:42.10 Forearm.seek :garage @22:26:43.06 Hand.close @22:26:44.16 Forearm.seek :retract @22:26:45.12 Elbow.reconfigure :tight @22:26:45.17 Elbow.seek CC @22:26:48.05 Elbow.reconfigure :loose @22:26:48.15 Forearm.reconfigure :tight @22:26:48.20 Forearm.seek CC @22:26:49.52 CC.seek :closed @22:26:54.05 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.47 @22:26:54.12 Hand.open @22:26:54.81 Forearm.reconfigure :loose @22:26:54.86 Forearm.seek :retract @22:26:55.83 PSV.dial PSR @22:26:56.90 Pulling 1.5ml of PSR.flush @22:26:56.91 PSR.select :flush @22:26:57.03 PS.seek 1.5ml @22:27:13.21 delay 5 seconds @22:27:18.23 PSV.dial :waste1 @22:27:18.72 PS.seek 0ml @22:27:34.86 PSV.dial PSR @22:27:35.35 PSR.select :air @22:27:35.47 PS.seek 2.5ml @22:28:01.81 PRV.dial CBV,avoiding: :ab2 @22:28:01.83
CBV.dial PRV @22:28:03.44 PSV.dial :mixloop @22:28:04.48 CTV.dial :air,avoiding: :intake @22:28:04.65 PTV.dial :PRVmixing @22:28:05.42 PS.seek 0ml @22:28:31.93 PSV.dial :air @22:28:32.40 PS.seek 2.5ml @22:28:58.71 PSV.dial :mixloop @22:28:59.18 PS.seek 0ml @22:29:26.02 PSV.dial :air @22:29:26.50 PS.seek 2.5ml @22:29:52.80 PSV.dial :mixloop @22:29:53.28 PS.seek 0ml @22:30:19.65 Garaging FlushPuck in Collection Clamp @22:30:19.72 Elbow.reconfigure :tight @22:30:19.77 Elbow.seek CC @22:30:20.11 Elbow.reconfigure :loose @22:30:20.21 Forearm.seek CC @22:30:21.16 Hand.close @22:30:21.74 CC.seek :open @22:30:26.39 Forearm.seek :retract @22:30:27.37 Elbow.reconfigure :tight @22:30:27.42 Elbow.seek :garage @22:30:30.36 Elbow.reconfigure :loose @22:30:30.46 Forearm.reconfigure :tight @22:30:30.51 Forearm.seek :garage @22:30:31.49 Hand.open @22:30:32.18 Forearm.reconfigure :loose @22:30:32.23 Forearm.seek :retract @22:30:33.16 delayUntil "22:34:40.37" @22:30:33.19 Whole Cell Archive sampling 1000ml @22:30:33.21 Loading wcr puck from tube 5 into Collection Clamp @22:30:33.45 SC.reconfigure :tight @22:30:33.50 SC.seek 5 @22:30:33.57 SC.configure :limp @22:30:33.79 Elbow.reconfigure :tight @22:30:33.85 Elbow.seek 5 @22:30:35.97 Forearm.reconfigure :tight @22:30:36.03 Forearm.seek 5 + 450 counts @22:30:37.46 SE.reconfigure :pick @22:30:37.54 SE.seek :beamBreak @22:30:38.84 SE.configure :default @22:30:39.00 Elbow.seek 5 @22:30:39.22 Forearm.seek 5 + 300 counts @22:30:39.51 Forearm.reconfigure :loose @22:30:39.55 Hand.close @22:30:40.13 SE.seek :down @22:30:40.33 Forearm.seek :retract @22:30:41.01 SE.stop @22:30:41.29 Elbow.seek CC @22:30:42.92 Elbow.reconfigure :loose @22:30:43.03 Forearm.reconfigure :tight @22:30:43.08 Forearm.seek CC @22:30:44.31 CC.seek :closed @22:30:48.84 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.39 @22:30:48.85 Hand.open @22:30:49.55 Forearm.reconfigure :loose @22:30:49.60 Forearm.seek :retract @22:30:50.56 collectSample 1000ml w/bubble point of 35psi @22:30:50.57 Priming 7ml @22:30:50.58 Intake.select :close @22:30:50.59 Exhaust.select :close @22:30:50.60 SS.hold @22:30:51.27 Calibrating pressure sensors @22:30:51.28 CTV.dial :air @22:30:51.30 SSV.dial :spit,avoiding: :exhaust @22:30:51.38 SS.reconfigure :fast @22:30:51.43 SS.seek 5ml @22:30:54.14 SSV.dial CBV @22:30:54.16 CBV.dial SSV @22:30:55.54 delay 3.8 seconds @22:30:59.48 Sensor limits: Intake max 102.6psia; Outlet max 95.0psia @22:30:59.49 SSV.dial :spit @22:31:00.83 SS.seek 0ml @22:31:04.02 SSV.dial CBV @22:31:05.35 CTV.dial :intake @22:31:06.13 SSV.dial CBV @22:31:06.16 CBV.dial SSV @22:31:06.30 Allowing 40s for 7.0ml of remaining 7.0ml @22:31:06.32 Intake.select :open @22:31:08.48 Intake @14.4psia, vacuum from 8.9 to 11.9psi @22:31:08.50 SS.reconfigure :inhaling 2.20ml/s @22:31:08.55 SS.seek 7ml @22:31:14.54 Stroke completed -- waiting for pressure to equalize ... @22:31:14.55 SS.reconfigure :exhale @22:31:14.60 SS.hold @22:31:35.02 Intake.select :close @22:31:35.18 SSV.dial :exhaust @22:31:35.95 Exhaust.select :open @22:31:36.07 SS.seek 0ml @22:31:40.38 Exhaust.select :close @22:31:40.49 Sampling 1000.0ml @22:31:40.51 Intake.select :close @22:31:40.52 Exhaust.select :close @22:31:40.53 SS.reconfigure :default @22:31:40.58 SS.hold @22:31:40.78 CTV.dial :intake @22:31:40.87 SSV.dial CBV @22:31:40.89 CBV.dial SSV @22:31:41.68 Allowing 130s for 25.0ml of remaining 1000.0ml @22:31:41.69 Intake.select :open @22:31:43.86 Intake @15.5psia, vacuum from 10.0 to 13.0psi @22:31:43.88 SS.reconfigure :inhaling 2.20ml/s @22:31:43.93 SS.seek 25ml @22:31:50.69 Vacuum too high -- waiting up to 40 seconds @22:31:50.70 SS.reconfigure :exhale @22:31:50.76 SS.hold @22:32:01.01 Intake @17.2psia, vacuum from 10.0 to 13.0psi @22:32:01.03 SS.reconfigure :inhaling 1.76ml/s @22:32:01.09 SS.seek 25ml @22:32:04.47 Vacuum too high -- waiting up to 40 seconds @22:32:04.48 SS.reconfigure :exhale @22:32:04.54 SS.hold @22:32:09.68 Intake @17.8psia, vacuum from 10.0 to 13.0psi @22:32:09.70 SS.reconfigure :inhaling 1.41ml/s @22:32:09.75 SS.seek 25ml @22:32:12.28 Vacuum too high -- waiting up to 40 seconds @22:32:12.30 SS.reconfigure :exhale @22:32:12.35 SS.hold @22:32:15.81 Intake @18.1psia, vacuum from 10.0 to 13.0psi @22:32:15.82 SS.reconfigure :inhaling 1.13ml/s @22:32:15.87 SS.seek 25ml @22:32:18.91 Vacuum too high -- waiting up to 40 seconds @22:32:18.92 SS.reconfigure :exhale @22:32:18.98 SS.hold @22:32:22.07 Intake @17.9psia, vacuum from 10.0 to 13.0psi @22:32:22.09 SS.reconfigure :inhaling 0.91ml/s @22:32:22.14 SS.seek 25ml @22:32:25.97 Vacuum too high -- waiting up to 40 seconds @22:32:25.98 SS.reconfigure :exhale @22:32:26.04 SS.hold @22:32:29.27 Intake @18.2psia, vacuum from 10.0 to 13.0psi @22:32:29.29 SS.reconfigure :inhaling 0.73ml/s @22:32:29.34 SS.seek 25ml @22:32:33.77 Stroke completed -- waiting for pressure to equalize ... @22:32:33.78 SS.reconfigure :exhale @22:32:33.83 SS.hold @22:33:11.10 Intake.select :close @22:33:11.26 SSV.dial :exhaust @22:33:12.02 Exhaust.select :open @22:33:12.14 SS.seek 0ml @22:33:21.02 Exhaust.select :close @22:33:21.13 SSV.dial CBV @22:33:21.16 CBV.dial SSV @22:33:21.93 Allowing 130s for 25.0ml of remaining 975.0ml @22:33:21.94 Intake.select :open @22:33:24.12 Intake @15.8psia, vacuum from 10.0 to 13.0psi @22:33:24.13 SS.reconfigure :inhaling 0.73ml/s @22:33:24.18 SS.seek 25ml @22:34:00.77 Stroke completed -- waiting for pressure to equalize ... @22:34:00.78 SS.reconfigure :exhale @22:34:00.83 SS.hold @22:34:40.38 recoverPRVreagent08 @22:34:40.39 PTV.dial :mixing @22:34:41.02 Intake.select :close @22:34:41.19 PBV.dial :waste1 @22:34:41.26 SSV.dial :exhaust @22:34:41.56 delay 30 seconds @22:34:42.02 Exhaust.select :open @22:34:42.14 SS.seek 0ml @22:34:50.97 Exhaust.select :close @22:34:51.08 SSV.dial CBV @22:34:51.10 CBV.dial SSV @22:34:51.88 Allowing 130s for 25.0ml of remaining 950.0ml @22:34:51.90 Intake.select :open @22:34:54.51 Intake @15.8psia, vacuum from 10.0 to 13.0psi @22:34:54.53 SS.reconfigure :inhaling 0.73ml/s @22:34:54.58 SS.seek 25ml @22:35:11.58 PBV.dial between PSV and waste1 @22:35:11.97 PSV.dial :air @22:35:12.44 PS.reconfigure :fast @22:35:12.49 PS.seek 2.5ml @22:35:31.13 Stroke completed -- waiting for pressure to equalize ... @22:35:31.14 SS.reconfigure :exhale @22:35:31.19 SS.hold @22:35:36.45 PSV.dial :mixloop @22:35:36.92 delay 2 seconds @22:35:38.93 PSV.dial :air @22:35:39.40 delay 2 seconds @22:35:41.42 PSV.dial :mixloop @22:35:41.89 delay 2 seconds @22:35:43.90 PSV.dial :air @22:35:44.38 PS.seek 0ml @22:36:06.38 Intake.select :close @22:36:06.54 SSV.dial :exhaust @22:36:07.32 Exhaust.select :open @22:36:07.43 SS.seek 0ml @22:36:07.86 washCycleDA #1 of 2 @22:36:07.87 PSR.select :air @22:36:07.88 PSV.dial PSR @22:36:09.23 Pulling 0.5ml of PSR.wash @22:36:09.24 PSR.select :wash @22:36:09.36 PS.reconfigure :default @22:36:09.41 PS.seek 0.5ml @22:36:15.42 delay 5 seconds @22:36:16.25 Exhaust.select :close @22:36:16.36 SSV.dial CBV @22:36:16.38 CBV.dial SSV @22:36:17.16 Allowing 130s for 25.0ml of remaining 925.0ml @22:36:17.18 Intake.select :open @22:36:19.34 Intake @15.9psia, vacuum from 10.0 to 13.0psi @22:36:19.36 SS.reconfigure :inhaling 0.73ml/s @22:36:19.41 SS.seek 25ml @22:36:20.43 PSR.select :air @22:36:20.56 PS.seek 2ml @22:36:36.70 PTV.dial :puck @22:36:37.45 PSV.dial PTV @22:36:38.20 PBV.dial :waste1 @22:36:38.63 PS.seek 1.5ml @22:36:44.57 delay 3 seconds @22:36:46.49 Vacuum too high -- waiting up to 40 seconds @22:36:46.51 SS.reconfigure :exhale @22:36:46.56 SS.hold @22:36:47.64 PBV.dial between PSV and waste1 @22:36:48.03 PS.seek 0ml @22:36:56.94 Intake @18.6psia, vacuum from 10.0 to 13.0psi @22:36:56.96 SS.reconfigure :inhaling 0.59ml/s @22:36:57.01 SS.seek 25ml @22:37:04.17 delay 2:00 @22:37:05.81 Vacuum too high -- waiting up to 40 seconds @22:37:05.82 SS.reconfigure :exhale @22:37:05.88 SS.hold @22:37:10.20 Intake @18.7psia, vacuum from 10.0 to 13.0psi @22:37:10.22 SS.reconfigure :inhaling 0.47ml/s @22:37:10.27 SS.seek 25ml @22:37:14.43 Stroke completed -- waiting for pressure to equalize ... @22:37:14.44 SS.reconfigure :exhale @22:37:14.49 SS.hold @22:37:33.27 Intake.select :close @22:37:33.43 SSV.dial :exhaust @22:37:34.64 Exhaust.select :open @22:37:34.76 SS.seek 0ml @22:37:43.61 Exhaust.select :close @22:37:43.73 SSV.dial CBV @22:37:43.75 CBV.dial SSV @22:37:44.53 Allowing 130s for 25.0ml of remaining 900.0ml @22:37:44.55 Intake.select :open @22:37:46.72 Intake @15.9psia, vacuum from 10.0 to 13.0psi @22:37:46.74 SS.reconfigure :inhaling 0.47ml/s @22:37:46.79 SS.seek 25ml @22:38:42.25 Stroke completed -- waiting for pressure to equalize ... @22:38:42.26 SS.reconfigure :exhale @22:38:42.31 SS.hold @22:39:03.12 Intake.select :close @22:39:03.28 SSV.dial :exhaust @22:39:04.05 Exhaust.select :open @22:39:04.17 SS.seek 0ml @22:39:04.22 recoverPSRreagent08 @22:39:04.23 PTV.dial :puck @22:39:04.31 PBV.dial :waste1 @22:39:04.68 delay 30 seconds @22:39:13.36 Exhaust.select :close @22:39:13.47 SSV.dial CBV @22:39:13.52 CBV.dial SSV @22:39:14.27 Allowing 130s for 25.0ml of remaining 875.0ml @22:39:14.28 Intake.select :open @22:39:16.45 Intake @16.0psia, vacuum from 10.0 to 13.0psi @22:39:16.47 SS.reconfigure :inhaling 0.47ml/s @22:39:16.52 SS.seek 25ml @22:39:34.70 PBV.dial between PSV and waste1 @22:39:35.09 PSR.select :air @22:39:35.11 PSV.dial PSR @22:39:35.87 PS.reconfigure :fast @22:39:35.92 PS.seek 2.5ml @22:39:59.79 PSV.dial PTV @22:40:00.56 delay 5 seconds @22:40:05.58 PSV.dial PSR @22:40:06.34 delay 5 seconds @22:40:11.36 PSV.dial PTV @22:40:11.50 Stroke completed -- waiting for pressure to equalize ... @22:40:11.51 SS.reconfigure :exhale @22:40:11.56 SS.hold @22:40:12.12 delay 5 seconds @22:40:17.13 PSV.dial :air @22:40:18.03 PS.seek 0ml @22:40:33.92 Intake.select :close @22:40:34.08 SSV.dial :exhaust @22:40:34.84 Exhaust.select :open @22:40:34.96 SS.seek 0ml @22:40:41.60 washCycleDA #2 of 2 @22:40:41.61 PSR.select :air @22:40:41.63 PSV.dial PSR @22:40:42.98 Pulling 0.5ml of PSR.wash @22:40:42.99 PSR.select :wash @22:40:43.10 PS.reconfigure :default @22:40:43.15 PS.seek 0.5ml @22:40:43.75 Exhaust.select :close @22:40:43.86 SSV.dial CBV @22:40:43.90 CBV.dial SSV @22:40:44.67 Allowing 130s for 25.0ml of remaining 850.0ml @22:40:44.69 Intake.select :open @22:40:46.86 Intake @15.8psia, vacuum from 10.0 to 13.0psi @22:40:46.88 SS.reconfigure :inhaling 0.47ml/s @22:40:46.93 SS.seek 25ml @22:40:49.13 delay 5 seconds @22:40:54.15 PSR.select :air @22:40:54.27 PS.seek 2ml @22:41:10.47 PTV.dial :puck @22:41:10.55 PSV.dial PTV @22:41:11.32 PBV.dial :waste1 @22:41:11.76 PS.seek 1.5ml @22:41:17.66 delay 3 seconds @22:41:21.06 PBV.dial between PSV and waste1 @22:41:21.50 PS.seek 0ml @22:41:37.66 delay 2:00 @22:41:42.34 Stroke completed -- waiting for pressure to equalize ... @22:41:42.35 SS.reconfigure :exhale @22:41:42.40 SS.hold @22:42:06.81 Intake.select :close @22:42:07.43 SSV.dial :exhaust @22:42:08.22 Exhaust.select :open @22:42:08.34 SS.seek 0ml @22:42:17.31 Exhaust.select :close @22:42:17.42 SSV.dial CBV @22:42:17.45 CBV.dial SSV @22:42:18.23 Allowing 130s for 25.0ml of remaining 825.0ml @22:42:18.25 Intake.select :open @22:42:20.41 Intake @15.6psia, vacuum from 10.0 to 13.0psi @22:42:20.43 SS.reconfigure :inhaling 0.47ml/s @22:42:20.48 SS.seek 25ml @22:43:15.60 Stroke completed -- waiting for pressure to equalize ... @22:43:15.62 SS.reconfigure :exhale @22:43:15.66 SS.hold @22:43:37.68 recoverPSRreagent08 @22:43:37.70 PTV.dial :puck @22:43:37.77 PBV.dial :waste1 @22:43:38.13 delay 30 seconds @22:43:40.64 Intake.select :close @22:43:40.80 SSV.dial :exhaust @22:43:41.57 Exhaust.select :open @22:43:41.69 SS.seek 0ml @22:43:50.73 Exhaust.select :close @22:43:50.84 SSV.dial CBV @22:43:50.89 CBV.dial SSV @22:43:51.64 Allowing 130s for 25.0ml of remaining 800.0ml @22:43:51.65 Intake.select :open @22:43:53.82 Intake @15.8psia, vacuum from 10.0 to 13.0psi @22:43:53.84 SS.reconfigure :inhaling 0.47ml/s @22:43:53.89 SS.seek 25ml @22:44:08.15 PBV.dial between PSV and waste1 @22:44:08.50 PSR.select :air @22:44:08.52 PSV.dial PSR @22:44:09.29 PS.reconfigure :fast @22:44:09.34 PS.seek 2.5ml @22:44:33.44 PSV.dial PTV @22:44:34.33 delay 5 seconds @22:44:39.35 PSV.dial PSR @22:44:40.10 delay 5 seconds @22:44:45.12 PSV.dial PTV @22:44:45.88 delay 5 seconds @22:44:49.05 Stroke completed -- waiting for pressure to equalize ... @22:44:49.07 SS.reconfigure :exhale @22:44:49.12 SS.hold @22:44:50.89 PSV.dial :air @22:44:51.66 PS.seek 0ml @22:45:15.50 getPMLReagent08 @22:45:15.51 PRV.dial :air @22:45:15.87 Intake.select :close @22:45:15.99 delay 5 seconds @22:45:16.07 SSV.dial :exhaust @22:45:16.85 Exhaust.select :open @22:45:16.97 SS.seek 0ml @22:45:21.00 PTV.dial :PRVmixing @22:45:22.33 delay 5 seconds @22:45:26.01 Exhaust.select :close @22:45:26.12 SSV.dial CBV @22:45:26.16 CBV.dial SSV @22:45:26.93 Allowing 130s for 25.0ml of remaining 775.0ml @22:45:26.95 Intake.select :open @22:45:27.35 PRV.dial :ab2 @22:45:27.81 PTV.dial :PRVmixing @22:45:27.83 PSV.dial :mixloop @22:45:28.32 PS.reconfigure :default @22:45:28.37 PS.seek 0.3ml @22:45:29.11 Intake @15.8psia, vacuum from 10.0 to 13.0psi @22:45:29.13 SS.reconfigure :inhaling 0.47ml/s @22:45:29.18 SS.seek 25ml @22:45:32.81 delay 2 seconds @22:45:34.83 PRV.dial :air @22:45:35.36 PS.seek 0.55ml @22:45:38.81 addPMLReagent08 @22:45:38.83 PSV.dial :air @22:45:39.32 PS.seek 2ml @22:45:54.94 PSV.dial :PML @22:45:55.42 PTV.dial :mixing @22:45:56.19 PBV.dial :waste1 @22:45:56.62 PS.seek 1.85ml @22:45:59.09 PBV.dial between PSV and waste1 @22:45:59.48 PS.seek 0ml @22:46:19.22 delay 2 seconds @22:46:21.24 delay 8:00 @22:46:24.29 Stroke completed -- waiting for pressure to equalize ... @22:46:24.31 SS.reconfigure :exhale @22:46:24.35 SS.hold @22:46:57.80 Intake.select :close @22:46:57.96 SSV.dial :exhaust @22:46:58.73 Exhaust.select :open @22:46:58.85 SS.seek 0ml @22:47:07.63 Exhaust.select :close @22:47:07.75 SSV.dial CBV @22:47:07.77 CBV.dial SSV @22:47:08.55 Allowing 130s for 25.0ml of remaining 750.0ml @22:47:08.57 Intake.select :open @22:47:10.74 Intake @15.6psia, vacuum from 10.0 to 13.0psi @22:47:10.76 SS.reconfigure :inhaling 0.47ml/s @22:47:10.81 SS.seek 25ml @22:48:06.25 Stroke completed -- waiting for pressure to equalize ... @22:48:06.27 SS.reconfigure :exhale @22:48:06.31 SS.hold @22:48:43.16 Intake.select :close @22:48:43.32 SSV.dial :exhaust @22:48:44.08 Exhaust.select :open @22:48:44.20 SS.seek 0ml @22:48:53.07 Exhaust.select :close @22:48:53.18 SSV.dial CBV @22:48:53.20 CBV.dial SSV @22:48:54.43 Allowing 130s for 25.0ml of remaining 725.0ml @22:48:54.45 Intake.select :open @22:48:56.62 Intake @15.7psia, vacuum from 10.0 to 13.0psi @22:48:56.64 SS.reconfigure :inhaling 0.47ml/s @22:48:56.69 SS.seek 25ml @22:49:49.26 Vacuum too high -- waiting up to 40 seconds @22:49:49.28 SS.reconfigure :exhale @22:49:49.33 SS.hold @22:50:14.47 Intake @17.4psia, vacuum from 10.0 to 13.0psi @22:50:14.49 SS.reconfigure :inhaling 0.38ml/s @22:50:14.54 SS.seek 25ml @22:50:18.41 Stroke completed -- waiting for pressure to equalize ... @22:50:18.42 SS.reconfigure :exhale @22:50:18.47 SS.hold @22:50:36.84 Intake.select :close @22:50:37.00 SSV.dial :exhaust @22:50:37.78 Exhaust.select :open @22:50:37.90 SS.seek 0ml @22:50:47.41 Exhaust.select :close @22:50:47.52 SSV.dial CBV @22:50:47.54 CBV.dial SSV @22:50:48.33 Allowing 130s for 25.0ml of remaining 700.0ml @22:50:48.35 Intake.select :open @22:50:50.52 Intake @15.6psia, vacuum from 10.0 to 13.0psi @22:50:50.54 SS.reconfigure :inhaling 0.38ml/s @22:50:50.59 SS.seek 25ml @22:51:58.50 Stroke completed -- waiting for pressure to equalize ... @22:51:58.51 SS.reconfigure :exhale @22:51:58.56 SS.hold @22:52:38.75 SS.reconfigure :default @22:52:38.81 SS.hold @22:52:38.88 Intake.select :close @22:52:39.04 ShallowSampler::Clogged in WCR -- Intake @16psia & Outlet @14psia won't equalize after 325.0ml @22:52:39.06 SSV.dial :exhaust @22:52:39.81 Exhaust.select :open @22:52:39.93 SS.reconfigure :exhale @22:52:39.98 SS.seek 0ml @22:52:48.76 Exhaust.select :close @22:52:48.87 Sampled 325.0ml @22:52:48.89 Drying puck before fixing for 5:00 @22:52:48.90 CBV.dial :waste2b @22:52:49.67 delay 1:00 @22:53:49.70 CSV.dial :air @22:53:50.16 CS.reconfigure :fast @22:53:50.22 CS.seek 10ml @22:54:14.84 Pumping wcr puck from tube 5 up to 35psi... @22:54:14.86 CBV.dial SSV @22:54:16.13 CBV.dial between CSV and waste2b @22:54:17.10 CSV.dial between air and CTV @22:54:17.43 CS.seek 7.349ml @22:54:21.26 recoverPRVreagent08 @22:54:21.27 PTV.dial :mixing @22:54:21.34 PBV.dial :waste1 @22:54:21.71 delay 30 seconds @22:54:24.38 CTV.dial CSV,avoiding: :air @22:54:24.40 CSV.dial CTV @22:54:25.36 CS.reconfigure :pressurize @22:54:25.42 CS.seek 0ml @22:54:44.74 CSV.dial :air @22:54:45.19 CS.reconfigure :fast @22:54:45.24 CS.seek 10ml @22:54:51.73 PBV.dial between PSV and waste1 @22:54:52.12 PSV.dial :air @22:54:52.59 PS.reconfigure :fast @22:54:52.64 PS.seek 2.5ml @22:55:09.84 CSV.dial between air and CTV @22:55:10.16 CS.seek 4.584ml @22:55:16.49 PSV.dial :mixloop @22:55:16.96 delay 2 seconds @22:55:18.98 PSV.dial :air @22:55:19.44 delay 2 seconds @22:55:21.46 PSV.dial :mixloop @22:55:21.96 delay 2 seconds @22:55:23.35 CTV.dial CSV,avoiding: :air @22:55:23.37 CSV.dial CTV @22:55:23.70 CS.reconfigure :pressurize @22:55:23.76 CS.seek 0ml @22:55:23.98 PSV.dial :air @22:55:24.45 PS.seek 0ml @22:55:35.99 CSV.dial :air @22:55:36.45 CS.reconfigure :fast @22:55:36.51 CS.seek 10ml @22:55:47.80 washCycleDA #1 of 3 @22:55:47.82 PSR.select :air @22:55:47.83 PSV.dial PSR @22:55:49.18 Pulling 0.5ml of PSR.wash @22:55:49.19 PSR.select :wash @22:55:49.30 PS.reconfigure :default @22:55:49.35 PS.seek 0.5ml @22:55:55.30 delay 5 seconds @22:56:00.32 PSR.select :air @22:56:00.45 PS.seek 2ml @22:56:01.02 CSV.dial between air and CTV @22:56:01.35 CS.seek 4.692ml @22:56:14.28 CTV.dial CSV,avoiding: :air @22:56:14.31 CSV.dial CTV @22:56:14.78 CS.reconfigure :pressurize @22:56:14.83 CS.seek 0ml @22:56:16.55 PTV.dial :puck @22:56:17.76 PSV.dial PTV @22:56:18.50 PBV.dial :waste1 @22:56:18.94 PS.seek 1.5ml @22:56:24.93 delay 3 seconds @22:56:27.34 CSV.dial :air @22:56:27.78 CS.reconfigure :fast @22:56:27.84 CS.seek 10ml @22:56:27.94 PBV.dial between PSV and waste1 @22:56:28.32 PS.seek 0ml @22:56:44.46 delay 2:00 @22:56:52.40 CSV.dial between air and CTV @22:56:52.72 CS.seek 4.713ml @22:57:05.63 CTV.dial CSV,avoiding: :air @22:57:05.65 CSV.dial CTV @22:57:06.01 CS.reconfigure :pressurize @22:57:06.07 CS.seek 0ml @22:57:18.65 CSV.dial :air @22:57:19.10 CS.reconfigure :default @22:57:19.15 CS.hold @22:57:19.28 ShallowSampler::Error in MAIN -- Puck.from 5, :wcr reached only 18.2 of 35psi after pumping in 40ml of gas @22:57:19.30 Waiting to Resume from Checkpoint "Pumping wcr puck from tube 5 up to 35psi..." @22:57:19.31 BadNews.email "Puck.from 5, :wcr reached only 18.2 of 35psi after pumping in 40ml of gas",Subject: "ShallowSampler::Error in MAIN" @22:57:23.64 upsync @22:58:44.48 recoverPSRreagent08 @22:58:44.49 PTV.dial :puck @22:58:44.58 PBV.dial :waste1 @22:58:44.96 delay 30 seconds @22:59:14.99 PBV.dial between PSV and waste1 @22:59:15.36 PSR.select :air @22:59:15.39 PSV.dial PSR @22:59:16.17 PS.reconfigure :fast @22:59:16.22 PS.seek 2.5ml @22:59:40.30 PSV.dial PTV @22:59:41.07 delay 5 seconds @22:59:46.09 PSV.dial PSR @22:59:46.85 delay 5 seconds @22:59:51.88 PSV.dial PTV @22:59:52.66 delay 5 seconds @22:59:57.68 PSV.dial :air @22:59:58.97 PS.seek 0ml @23:00:22.33 washCycleDA #2 of 3 @23:00:22.34 PSR.select :air @23:00:22.36 PSV.dial PSR @23:00:23.70 Pulling 0.5ml of PSR.wash @23:00:23.71 PSR.select :wash @23:00:23.83 PS.reconfigure :default @23:00:23.88 PS.seek 0.5ml @23:00:29.83 delay 5 seconds @23:00:34.85 PSR.select :air @23:00:34.97 PS.seek 2ml @23:00:51.08 PTV.dial :puck @23:00:51.09 PSV.dial PTV @23:00:51.95 PBV.dial :waste1 @23:00:52.39 PS.seek 1.5ml @23:00:58.36 delay 3 seconds @23:01:01.38 PBV.dial between PSV and waste1 @23:01:01.74 PS.seek 0ml @23:01:17.89 delay 2:00 @23:03:17.92 recoverPSRreagent08 @23:03:17.93 PTV.dial :puck @23:03:17.94 PBV.dial :waste1 @23:03:18.30 delay 30 seconds @23:03:48.32 PBV.dial between PSV and waste1 @23:03:48.68 PSR.select :air @23:03:48.70 PSV.dial PSR @23:03:49.45 PS.reconfigure :fast @23:03:49.51 PS.seek 2.5ml @23:04:13.35 PSV.dial PTV @23:04:14.12 delay 5 seconds @23:04:19.14 PSV.dial PSR @23:04:19.90 delay 5 seconds @23:04:24.92 PSV.dial PTV @23:04:25.68 delay 5 seconds @23:04:30.70 PSV.dial :air @23:04:31.49 PS.seek 0ml @23:04:54.79 washCycleDA #3 of 3 @23:04:54.80 PSR.select :air @23:04:54.82 PSV.dial PSR @23:04:56.15 Pulling 0.5ml of PSR.wash @23:04:56.16 PSR.select :wash @23:04:56.28 PS.reconfigure :default @23:04:56.33 PS.seek 0.5ml @23:05:02.35 delay 5 seconds @23:05:07.37 PSR.select :air @23:05:07.50 PS.seek 2ml @23:05:23.60 PTV.dial :puck @23:05:23.62 PSV.dial PTV @23:05:24.37 PBV.dial :waste1 @23:05:24.80 PS.seek 1.5ml @23:05:30.79 delay 3 seconds @23:05:33.81 PBV.dial between PSV and waste1 @23:05:34.19 PS.seek 0ml @23:05:50.32 delay 2:00 @23:07:50.35 recoverPSRreagent08 @23:07:50.36 PTV.dial :puck @23:07:50.37 PBV.dial :waste1 @23:07:50.74 delay 30 seconds @23:08:20.77 PBV.dial between PSV and waste1 @23:08:21.15 PSR.select :air @23:08:21.16 PSV.dial PSR @23:08:21.93 PS.reconfigure :fast @23:08:21.98 PS.seek 2.5ml @23:08:45.88 PSV.dial PTV @23:08:46.65 delay 5 seconds @23:08:51.67 PSV.dial PSR @23:08:52.90 delay 5 seconds @23:08:57.92 PSV.dial PTV @23:08:58.68 delay 5 seconds @23:09:03.70 PSV.dial :air @23:09:04.48 PS.seek 0ml @23:09:27.78 Gate.power :camera,:ON @23:09:27.80 Mixing DA Substrates @23:09:27.81 PSR.select :air @23:09:27.83 PSV.dial PSR @23:09:29.24 Pulling 0.25ml of PSR.sub1 @23:09:29.26 PSR.select :sub1 @23:09:29.47 PS.reconfigure :default @23:09:29.59 PS.seek 0.25ml @23:09:33.16 delay 5 seconds @23:09:38.18 Pulling 0.25ml of PSR.sub2 @23:09:38.20 PSR.select :sub2 @23:09:38.31 PS.seek 0.5ml @23:09:41.75 delay 5 seconds @23:09:46.77 PSR.select :air @23:09:46.89 PS.seek 2ml @23:10:03.13 PTV.dial :puck @23:10:03.14 PSV.dial PTV @23:10:04.02 PBV.dial :waste1 @23:10:04.47 PS.seek 1.5ml @23:10:10.38 delay 3 seconds @23:10:13.40 PBV.dial between PSV and waste1 @23:10:13.78 PS.seek 0ml @23:10:29.91 delay 10 seconds @23:10:39.94 recoverPSRreagent08 @23:10:39.95 PTV.dial :puck @23:10:39.96 PBV.dial :waste1 @23:10:40.33 delay 30 seconds @23:11:10.36 PBV.dial between PSV and waste1 @23:11:10.74 PSR.select :air @23:11:10.76 PSV.dial PSR @23:11:11.52 PS.reconfigure :fast @23:11:11.58 PS.seek 2.5ml @23:11:35.69 PSV.dial PTV @23:11:36.47 delay 5 seconds @23:11:41.49 PSV.dial PSR @23:11:42.25 delay 5 seconds @23:11:47.28 PSV.dial PTV @23:11:48.03 delay 5 seconds @23:11:53.06 PSV.dial :air @23:11:53.84 PS.seek 0ml @23:12:17.72 Additional air clear @23:12:17.73 PSR.select :air @23:12:17.75 PSV.dial PSR @23:12:19.11 Pulling 2ml of PSR.air @23:12:19.12 PSR.select :air @23:12:19.14 PS.reconfigure :default @23:12:19.19 PS.seek 2ml @23:12:40.47 delay 5 seconds @23:12:45.49 PTV.dial :puck @23:12:45.51 PSV.dial PTV @23:12:46.28 PBV.dial :waste1 @23:12:46.72 PS.seek 1.5ml @23:12:52.66 delay 3 seconds @23:12:55.68 PBV.dial between PSV and waste1 @23:12:56.05 delay 1 second @23:12:57.07 PSV.dial :waste1 @23:12:58.15 PS.seek 0ml @23:13:14.34 Processing Puck Heater at 28.1C before imaging @23:13:14.35 PH.coast @23:13:14.46 Imaging da puck in Processing Clamp while storing it to tube 4 @23:13:15.12 SC.reconfigure :tight @23:13:15.17 SC.seek 4 @23:13:18.73 SC.configure :limp @23:13:18.95 Elbow.reconfigure :tight @23:13:19.00 Elbow.seek 4 @23:13:20.77 Forearm.reconfigure :tight @23:13:20.83 Forearm.seek 4 + 450 counts @23:13:21.98 SE.reconfigure :pick @23:13:22.04 SE.seek :beamBreak @23:13:25.02 SE.configure :default @23:13:25.18 Elbow.reconfigure :limp @23:13:25.23 Forearm.reconfigure :loose @23:13:25.28 Forearm.seek 4 @23:13:25.50 Elbow.hold @23:13:25.57 Elbow.reconfigure :tight @23:13:25.68 SE.seek 2.17 Pucks below Top Plate @23:13:26.23 Forearm.force -20 @23:13:26.28 Forearm.seek 4 + 309 counts @23:13:26.55 Forearm.reconfigure :tight @23:13:26.61 Forearm.seek 4 + 450 counts @23:13:26.82 SE.seek 3.17 Pucks below Top Plate @23:13:27.35 Forearm.reconfigure :loose @23:13:27.40 Forearm.seek :retract @23:13:28.35 Elbow.seek PC @23:13:30.88 Elbow.reconfigure :loose @23:13:30.98 Forearm.seek PC @23:13:31.94 Hand.close @23:13:32.58 PC.seek :open @23:13:37.36 Forearm.seek :retract @23:13:38.30 Elbow.reconfigure :tight @23:13:38.35 Elbow.seek 4 @23:13:41.50 Forearm.reconfigure :tight @23:13:41.55 Forearm.seek 4 + 450 counts @23:13:42.52 Hand.open @23:13:43.14 SE.seek 2.82 Pucks below Top Plate @23:13:43.42 SE.reconfigure :seal @23:13:43.47 SE.seek 1.86 Pucks below Top Plate @23:13:44.06 Lights, camera, ACTION! @23:13:44.10 PuckCamera: snap -bin=2,2 -auto /var/log/esp/da23dec0123h775ml.tif @23:14:03.53 Waiting >20 seconds for da to relinquish FlushPuck @23:14:04.39 SX HX9/16 (USB:13): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 16.981 seconds /var/log/esp/da23dec0123h775ml.tif: TIFF Upload Complete @23:14:04.42 PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da23dec0123h775ml15s.tif @23:14:20.07 SX HX9/16 (USB:13): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15 seconds /var/log/esp/midres/da23dec0123h775ml15s.tif: TIFF Upload Complete @23:14:20.10 PuckCamera: snap -bin=2,2 20.0 /var/log/esp/midres/da23dec0123h775ml20s.tif @23:14:40.71 SX HX9/16 (USB:13): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 20 seconds /var/log/esp/midres/da23dec0123h775ml20s.tif: TIFF Upload Complete @23:14:40.72 Gate.power :camera,:OFF @23:14:40.75 DAfans.email "16.981 sec auto-exposure",Subject: "da23dec0123h775ml images!" @23:14:41.01 Elbow.seek 4 @23:14:41.43 Forearm.seek 4 + 450 counts @23:14:41.66 SE.reconfigure :gently @23:14:41.72 SE.seek 2.57 Pucks below Top Plate @23:14:42.50 Elbow.reconfigure :limp @23:14:42.59 Forearm.reconfigure :loose @23:14:42.64 Forearm.seek 4 @23:14:42.92 Elbow.hold @23:14:42.99 Elbow.reconfigure :tight @23:14:43.05 SE.reconfigure :default @23:14:43.10 SE.seek 2.76 Pucks below Top Plate @23:14:43.53 Forearm.force -20 @23:14:43.58 Forearm.seek 4 + 288 counts @23:14:44.07 Forearm.reconfigure :tight @23:14:44.17 Forearm.seek 4 + 450 counts @23:14:44.54 SE.seek :down @23:14:45.87 Forearm.reconfigure :loose @23:14:45.97 Forearm.seek :retract @23:14:47.11 SE.stop @23:14:48.50 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @23:14:48.89 Elbow.seek FlushPuck::Garage @23:14:50.89 Elbow.reconfigure :loose @23:14:51.24 Forearm.seek :garage @23:14:52.31 Hand.close @23:14:53.21 Forearm.seek :retract @23:14:54.25 Elbow.reconfigure :tight @23:14:54.35 Elbow.seek PC @23:14:55.75 Elbow.reconfigure :loose @23:14:56.86 Forearm.reconfigure :tight @23:14:56.95 Forearm.seek PC @23:14:58.47 PC.seek :closed @23:15:03.31 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.67 @23:15:03.45 Hand.open @23:15:04.29 Forearm.reconfigure :loose @23:15:04.39 Forearm.seek :retract @23:15:05.38 PSR.select :air @23:15:05.42 PSV.dial PSR @23:15:05.95 Pulling 0.5ml of PSR.flush @23:15:05.97 PSR.select :flush @23:15:06.16 PS.seek 0.5ml @23:15:12.45 delay 5 seconds @23:15:15.22 upsync @23:15:17.48 PSR.select :air @23:15:17.61 PS.seek 2.5ml @23:15:38.87 delay 1 second @23:15:39.89 PRV.dial :waste2,avoiding: :ab2 @23:15:41.76 PTV.dial :PRVmixing @23:15:43.09 PSV.dial :mixloop @23:15:44.15 PS.seek :empty @23:16:10.46 PSR.select :air @23:16:10.48 PSV.dial PSR @23:16:11.59 Pulling 0.5ml of PSR.flush @23:16:11.60 PSR.select :flush @23:16:11.72 PS.seek 0.5ml @23:16:17.65 delay 5 seconds @23:16:22.67 PSR.select :air @23:16:22.80 PS.seek 2.5ml @23:16:44.03 delay 1 second @23:16:45.05 PBV.dial :waste1 @23:16:45.42 PTV.dial :mixing @23:16:46.17 PSV.dial :mixloop @23:16:47.36 PS.seek :empty @23:17:13.74 PSV.dial PSR @23:17:14.79 PSR.select :flush @23:17:14.92 PS.seek 1ml @23:17:25.96 PSR.select :air @23:17:26.08 PS.seek 2.5ml @23:17:42.24 PSV.dial PTV @23:17:43.01 PTV.dial :puck @23:17:43.76 PBV.dial :waste1 @23:17:43.85 PS.seek 0ml @23:18:10.18 PSV.dial PSR @23:18:10.95 PSR.select :flush @23:18:11.07 PS.seek 0.5ml @23:18:17.09 PSR.select :air @23:18:17.21 PS.seek 2.5ml @23:18:38.46 PSV.dial PTV @23:18:39.23 PBV.dial PSV @23:18:39.80 PS.seek 0ml @23:19:06.18 PSV.dial PBV @23:19:06.66 PS.seek 2.5ml @23:19:32.96 PSV.dial :waste1 @23:19:33.70 PS.seek 0ml @23:20:00.08 PSV.dial PSR @23:20:00.55 PSR.select :air @23:20:00.58 PS.seek 2.5ml @23:20:26.93 PSV.dial PTV @23:20:27.66 PBV.dial PSV @23:20:27.75 PS.seek 0ml @23:20:54.11 PSV.dial PBV @23:20:54.59 PS.seek 2.5ml @23:21:20.96 PSV.dial :waste1 @23:21:21.72 PS.seek 0ml @23:21:48.02 PSV.dial PSR @23:21:48.49 PSR.select :flush @23:21:48.61 PS.seek 1ml @23:21:59.68 PSR.select :air @23:22:00.24 PS.seek 2.5ml @23:22:16.40 PSV.dial PTV @23:22:17.13 PTV.dial :puck @23:22:17.21 PBV.dial :waste1 @23:22:17.78 PS.seek 0ml @23:22:44.14 PSV.dial PSR @23:22:44.91 PSR.select :flush @23:22:45.04 PS.seek 0.5ml @23:22:51.02 PSR.select :air @23:22:51.14 PS.seek 2.5ml @23:23:12.71 PSV.dial PTV @23:23:13.47 PBV.dial PSV @23:23:14.04 PS.seek 0ml @23:23:40.39 PSV.dial PBV @23:23:40.87 PS.seek 2.5ml @23:24:07.21 PSV.dial :waste1 @23:24:07.95 PS.seek 0ml @23:24:34.33 PSV.dial PSR @23:24:34.80 PSR.select :air @23:24:34.83 PS.seek 2.5ml @23:25:01.24 PSV.dial PTV @23:25:01.97 PBV.dial PSV @23:25:02.17 PS.seek 0ml @23:25:28.70 PSV.dial PBV @23:25:29.18 PS.seek 2.5ml @23:25:55.61 PSV.dial :waste1 @23:25:56.37 PS.seek 0ml @23:26:22.68 Garaging FlushPuck in Processing Clamp @23:26:22.75 Elbow.reconfigure :tight @23:26:22.80 Elbow.seek PC @23:26:22.92 Elbow.reconfigure :loose @23:26:23.02 Forearm.seek PC @23:26:23.99 Hand.close @23:26:24.57 PC.seek :open @23:26:29.29 Forearm.seek :retract @23:26:30.26 Elbow.reconfigure :tight @23:26:30.31 Elbow.seek :garage @23:26:31.75 Elbow.reconfigure :loose @23:26:31.85 Forearm.reconfigure :tight @23:26:32.28 Forearm.seek :garage @23:26:33.30 Hand.open @23:26:34.01 Forearm.reconfigure :loose @23:26:34.05 Forearm.seek :retract @23:32:30.61 Session Begun @23:32:37.03 -> showlog -600 @23:33:25.05 -> CC Collection Clamp contains wcr puck from tube 5 @23:33:29.73 -> PC Processing Clamp open @23:33:32.37 -> Flush FlushPuck is in FlushPuck::Garage @23:35:14.48 -> Puck.count @23:35:14.57 SE.seek :down @23:35:14.65 SE.stop @23:35:14.76 SC.reconfigure :tight @23:35:14.84 SC.seek 1 @23:35:24.82 SC.configure :limp @23:35:25.07 Elbow.reconfigure :tight @23:35:25.14 Elbow.seek 1 @23:35:27.03 Forearm.reconfigure :tight @23:35:27.10 Forearm.seek 1 + 450 counts @23:35:28.20 SE.reconfigure :place @23:35:28.28 SE.seek :beamBreak @23:35:29.42 SE.configure :default @23:35:29.56 SE.seek :down @23:35:30.03 SE.stop @23:35:30.69 Forearm.reconfigure :loose @23:35:30.76 Forearm.seek :retract @23:35:31.71 SC.reconfigure :tight @23:35:31.78 SC.seek 2 @23:35:35.41 SC.configure :limp @23:35:35.59 Elbow.seek 2 @23:35:35.80 Forearm.reconfigure :tight @23:35:35.87 Forearm.seek 2 + 450 counts @23:35:36.95 SE.reconfigure :place @23:35:37.04 SE.seek :beamBreak @23:35:38.28 SE.configure :default @23:35:38.41 SE.seek :down @23:35:38.95 SE.stop @23:35:39.54 Forearm.reconfigure :loose @23:35:39.62 Forearm.seek :retract @23:35:40.61 SC.reconfigure :tight @23:35:40.68 SC.seek 3 @23:35:44.29 SC.configure :limp @23:35:44.48 Elbow.seek 3 @23:35:44.91 Forearm.reconfigure :tight @23:35:44.99 Forearm.seek 3 + 450 counts @23:35:46.03 SE.reconfigure :place @23:35:46.12 SE.seek :beamBreak @23:35:47.25 SE.configure :default @23:35:47.32 Puck::Error in dave -- 33 pucks loaded in tube 3 when expecting 31 @23:35:47.43 SE.seek :down @23:35:47.87 SE.stop @23:35:48.56 Forearm.reconfigure :loose @23:35:48.63 Forearm.seek :retract @23:35:49.58 SC.reconfigure :tight @23:35:49.66 SC.seek 4 @23:35:53.25 SC.configure :limp @23:35:53.42 Elbow.seek 4 @23:35:54.05 Forearm.reconfigure :tight @23:35:54.13 Forearm.seek 4 + 450 counts @23:35:55.23 SE.reconfigure :place @23:35:55.31 SE.seek :beamBreak @23:35:58.40 SE.configure :default @23:35:58.53 SE.seek :down @23:35:59.66 Forearm.reconfigure :loose @23:35:59.73 Forearm.seek :retract @23:36:01.01 SE.stop @23:36:01.07 SC.reconfigure :tight @23:36:01.14 SC.seek 5 @23:36:04.67 SC.configure :limp @23:36:04.85 Elbow.seek 5 @23:36:05.15 Forearm.reconfigure :tight @23:36:05.23 Forearm.seek 5 + 450 counts @23:36:06.28 SE.reconfigure :place @23:36:06.81 SE.seek :beamBreak @23:36:08.36 SE.configure :default @23:36:08.50 SE.seek :down @23:36:09.32 SE.stop @23:36:09.63 Forearm.reconfigure :loose @23:36:09.71 Forearm.seek :retract @23:36:10.68 SC.reconfigure :tight @23:36:10.75 SC.seek 6 @23:36:14.29 SC.configure :limp @23:36:14.47 Elbow.seek 6 @23:36:14.68 Forearm.reconfigure :tight @23:36:14.77 Forearm.seek 6 + 450 counts @23:36:15.81 SE.reconfigure :place @23:36:15.89 SE.seek :beamBreak @23:36:17.08 SE.configure :default @23:36:17.21 SE.seek :down @23:36:17.63 SE.stop @23:36:18.34 Forearm.reconfigure :loose @23:36:18.42 Forearm.seek :retract @23:36:19.44 SC.reconfigure :tight @23:36:19.52 SC.seek 7 @23:36:23.07 SC.configure :limp @23:36:23.26 Elbow.seek 7 @23:36:23.56 Forearm.reconfigure :tight @23:36:23.64 Forearm.seek 7 + 450 counts @23:36:24.68 SE.reconfigure :place @23:36:24.77 SE.seek :beamBreak @23:36:26.08 SE.configure :default @23:36:26.21 SE.seek :down @23:36:26.88 SE.stop @23:36:27.35 Forearm.reconfigure :loose @23:36:27.42 Forearm.seek :retract {1 => 33, 2 => 33, 3 => 33, 4 => 4, 5 => 28, 6 => 33, 7 => 31} @23:39:28.16 -> CC.unloadPuck 4, :WCR @23:39:28.24 Puck::Warning in dave -- Unloading Puck.from 5, :wcr to Storage Carousel @23:39:28.27 Storing WCR puck in Collection Clamp to tube 4 @23:39:28.47 SC.reconfigure :tight @23:39:28.54 SC.seek 4 @23:39:38.45 SC.configure :limp @23:39:39.18 Elbow.seek 4 @23:39:39.71 Forearm.reconfigure :tight @23:39:39.78 Forearm.seek 4 + 450 counts @23:39:40.95 SE.reconfigure :pick @23:39:41.03 SE.seek :beamBreak @23:39:44.02 SE.configure :default @23:39:44.21 Elbow.reconfigure :limp @23:39:44.28 Forearm.reconfigure :loose @23:39:44.35 Forearm.seek 4 @23:39:44.64 Elbow.hold @23:39:44.72 Elbow.reconfigure :tight @23:39:44.84 SE.seek 3.16 Pucks below Top Plate @23:39:45.30 Forearm.force -20 @23:39:45.36 Forearm.seek 4 + 298 counts @23:39:45.74 Forearm.reconfigure :tight @23:39:45.81 Forearm.seek 4 + 450 counts @23:39:46.03 SE.seek 4.16 Pucks below Top Plate @23:39:46.64 Forearm.reconfigure :loose @23:39:46.71 Forearm.seek :retract @23:39:47.70 Elbow.seek CC @23:39:49.37 Elbow.reconfigure :loose @23:39:49.49 Forearm.seek CC @23:39:50.50 Hand.close @23:39:51.19 CC.seek :open @23:39:55.96 Forearm.seek :retract @23:39:56.97 Elbow.reconfigure :tight @23:39:57.04 Elbow.seek 4 @23:39:58.84 Elbow.reconfigure :limp @23:39:58.92 Forearm.seek 4 @23:39:59.95 Elbow.hold @23:40:00.03 Elbow.reconfigure :tight @23:40:00.10 Hand.open @23:40:00.81 Forearm.force -20 @23:40:00.87 Forearm.seek 4 + 312 counts @23:40:01.18 Forearm.reconfigure :tight @23:40:01.64 Forearm.seek 4 + 450 counts @23:40:01.99 SE.seek :down @23:40:03.12 Forearm.reconfigure :loose @23:40:03.19 Forearm.seek :retract @23:40:04.43 SE.stop @23:40:29.58 -> purgeIntake @23:40:29.60 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @23:40:29.82 Elbow.seek FlushPuck::Garage @23:40:31.65 Elbow.reconfigure :loose @23:40:31.77 Forearm.seek :garage @23:40:32.75 Hand.close @23:40:33.55 Forearm.seek :retract @23:40:34.53 Elbow.reconfigure :tight @23:40:34.60 Elbow.seek CC @23:40:37.58 Elbow.reconfigure :loose @23:40:37.70 Forearm.reconfigure :tight @23:40:37.77 Forearm.seek CC @23:40:39.07 CC.seek :closed @23:40:43.64 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.30 @23:40:43.73 Hand.open @23:40:44.37 Forearm.reconfigure :loose @23:40:44.44 Forearm.seek :retract @23:40:45.39 Purging external Intake @23:40:45.54 CSV.dial :flush @23:40:46.00 CS.reconfigure :fast @23:40:46.08 CS.seek 10ml @23:41:10.79 delay 5 seconds @23:41:15.82 CSV.dial CBV @23:41:15.89 CBV.dial CSV @23:41:16.64 CTV.dial :intake @23:41:17.43 Intake.select :open @23:41:17.56 CS.seek 0ml @23:41:41.23 Intake.select :close @23:41:41.40 CTV.dial :air @23:41:42.70 Garaging FlushPuck in Collection Clamp @23:41:42.80 Elbow.reconfigure :tight @23:41:42.87 Elbow.seek CC @23:41:43.20 Elbow.reconfigure :loose @23:41:43.32 Forearm.seek CC @23:41:44.37 Hand.close @23:41:45.06 CC.seek :open @23:41:49.72 Forearm.seek :retract @23:41:50.78 Elbow.reconfigure :tight @23:41:50.85 Elbow.seek :garage @23:41:53.77 Elbow.reconfigure :loose @23:41:53.89 Forearm.reconfigure :tight @23:41:53.96 Forearm.seek :garage @23:41:55.02 Hand.open @23:41:55.74 Forearm.reconfigure :loose @23:41:55.81 Forearm.seek :retract FlushPuck::Garage @23:42:57.62 -> abort! 'mission complete' @23:42:57.64 CmdServer::Client::Error in dave -- Aborting Mission: mission complete @23:42:57.68 BadNews.email "mission complete",Subject: "DA_WCR_120123b ABORTED!" # @23:43:01.82 upsync @23:43:01.92 Session Ended + ESPwhich DA_WCR_120423.rb + fn=DA_WCR_120423.rb + IFS=: + set /home/esp/esp2/mission . + fullPath=/home/esp/esp2/mission/DA_WCR_120423.rb + [ -f /home/esp/esp2/mission/DA_WCR_120423.rb ] + realpath /home/esp/esp2/mission/DA_WCR_120423.rb + return 0 + scriptPath=/home/esp/esp2local/mission/DA_WCR_120423.rb + exec ruby /home/esp/esp2/lib/esp.rb /home/esp/esp2local/mission/DA_WCR_120423.rb @13:19:06.65UTC04-Dec-23
ESP2 v3.42 I2C Gateway v3.20 for core bus @13:19:09.26 Configuration for short puck jake 1/11/21 by brent@mbari.org /home/esp/esp2/type/shallow/jake/configure.rb @13:19:13.20 Polling Can every 10:00 @13:19:13.36 Cleanly exited at 13:16:26UTC04-Dec-23 @13:19:13.40 Loading /home/esp/esp2local/mission/DA_WCR_120423.rb mission @13:19:13.46 GoodNews.email "Starting DA_WCR_120423 w/pucks in tube 5\n{1 => 33,\n 2 => 33,\n 3 => 33,\n 4 => 5,\n 5 => 28,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@13:19:13, 31% flow, 16.6C, 30% humidity, 15.1psia, 13.730V, 0.345A, 145.129Ah, 4.73W",Subject: "DA_WCR_120423 begun" @13:19:13.49
Preparing to sleep until + 00:00:05 @13:19:13.54 Asleep til + 00:00:05 @13:19:13.56 Continuing at 13:19 @13:19:13.84 Gate.power :core,:OFF @13:19:13.86 Gate.power :raw,:OFF @13:19:13.87 Shutdown @13:19:16.82 GoodNews.email "Then, in tube 5: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_120423.rb:14\n{1 => 33,\n 2 => 33,\n 3 => 31,\n 4 => 5,\n 5 => 28,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@13:19:13, 31% flow, 16.7C, 30% humidity, 15.1psia, 13.730V, 0.345A, 145.129Ah, 4.73W",Subject: "Asleep until 13:19" @13:19:18.15 upsync @13:19:18.46
Powering core bus @13:19:21.35 upsync @13:19:24.01
Gate.power :raw,:ON @13:19:24.84 CS.configure :default @13:19:24.89 PS.configure :default @13:19:24.93 SS.configure :default @13:19:24.98 CC.configure :default @13:19:25.03 PC.configure :default @13:19:25.10 Forearm.configure :loose @13:19:25.15 Elbow.configure :loose @13:19:25.21 SC.configure :loose @13:19:25.26 SE.configure :default @13:19:25.31 CH.configure :default @13:19:25.35 PH.configure :default @13:19:25.39 All dwarf controllers running firmware version 3.85 @13:19:25.84 Valve.reset @13:19:26.15 Intake.select :close @13:19:26.19 Exhaust.select :close @13:19:26.21 CTV.dial :air @13:19:26.32 PSV.dial :air @13:19:26.36 CSV.dial :waste1 @13:19:26.46
Forearm.home.seek :retract @13:19:26.84 SSV.dial :spit,avoiding: :exhaust @13:19:27.26 Hand.close @13:19:27.30
Elbow.reconfigure :loose @13:19:27.38 Elbow.jog 1500 @13:19:27.46 PS.home.seek 0ml @13:19:27.70 CS.reconfigure :fast @13:19:27.73 SS.home.seek 0ml @13:19:27.84 CS.home.seek 0ml @13:19:27.92
Elbow.reconfigure :loose @13:19:27.97 Elbow.home.seek :clear @13:19:31.29 SE.home.seek :down @13:19:31.92 SC.reconfigure :tight @13:19:31.98 SC.home.seek :initial @13:19:35.77 PS.stop @13:19:50.93
SC.configure :limp @13:19:50.98 Forearm.seek :retract @13:19:51.05 Elbow.seek :hammer @13:19:52.16 Forearm.reconfigure :tight @13:19:52.21 Forearm.seek retracted @13:19:52.49 PC.home.seek :open @13:19:52.51
CC.home.seek :open @13:19:56.00 Phase [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] @13:19:56.01 Notice.email "At 13:19:55 began in tube 5: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_120423.rb:14\n{1 => 33,\n 2 => 33,\n 3 => 31,\n 4 => 5,\n 5 => 28,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@13:19:55, 31% flow, 16.7C, 30% humidity, 15.1psia, 13.695V, 0.290A, 145.133Ah, 3.98W",Subject: "Started DA_WCR_120423 phase initialPurge; da [1000, 1000] #da and wsr are run simultanelously" @13:19:56.02
Starting initialPurge @13:19:56.10 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @13:19:56.17 Hand.open @13:19:56.90 Elbow.reconfigure :tight @13:19:56.95 Elbow.seek FlushPuck::Garage @13:19:58.80 Elbow.reconfigure :loose @13:19:58.90 Forearm.reconfigure :loose @13:19:58.95 Forearm.seek :garage @13:19:59.94 Hand.close @13:20:00.62 upsync @13:20:00.70
Forearm.seek :retract @13:20:01.72 Elbow.reconfigure :tight @13:20:01.77 Elbow.seek CC @13:20:04.80 Elbow.reconfigure :loose @13:20:04.91 Forearm.reconfigure :tight @13:20:04.96 Forearm.seek CC @13:20:06.15 CC.seek :closed @13:20:10.71 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.39 @13:20:10.79 Hand.open @13:20:11.46 Forearm.reconfigure :loose @13:20:11.51 Forearm.seek :retract @13:20:12.47 Flushing the Collection Valve::Manifold @13:20:12.59 CSV.dial CSR @13:20:13.88 Pulling 5ml of CSR.flush @13:20:13.89 CSR.select :flush @13:20:14.02 CS.reconfigure :default @13:20:14.47 CS.seek 5ml @13:20:28.32 delay 5 seconds @13:20:33.34 CSR.select :air @13:20:33.51 CS.reconfigure :fast @13:20:33.56 CS.seek 7.499ml @13:20:40.47 CSV.dial :waste @13:20:41.78 CS.reconfigure :default @13:20:41.82 CS.seek 0ml @13:21:01.88 Purging external Intake @13:21:02.00 CSV.dial :flush @13:21:02.46 CS.reconfigure :fast @13:21:02.51 CS.seek 10ml @13:21:27.01 delay 5 seconds @13:21:32.03 CSV.dial CBV @13:21:32.07
CBV.dial CSV @13:21:32.80 CTV.dial :intake @13:21:33.60 Intake.select :open @13:21:33.72 CS.seek 0ml @13:21:57.28 Intake.select :close @13:21:57.39 CTV.dial :air @13:21:58.16 Flushing upper manifold @13:21:58.28 CSV.dial :flush @13:21:59.03 CS.seek 5ml @13:22:11.69 delay 5 seconds @13:22:16.71 CSV.dial :air @13:22:17.24 CS.seek 7.001ml @13:22:22.76 CSV.dial CBV @13:22:22.79
CBV.dial CSV @13:22:23.82 CTV.dial :air @13:22:23.84 CS.seek 0ml @13:22:40.47 delay 7 seconds @13:22:47.50 CS.seek 3ml @13:22:55.63 CSV.dial :waste @13:22:56.09 CS.seek 0ml @13:23:03.89 CSV.dial :flush @13:23:04.46 CS.seek 3ml @13:23:12.54 delay 5 seconds @13:23:17.56 CSV.dial :air @13:23:18.10 CS.seek 5ml @13:23:23.81 CSV.dial :air @13:23:24.01 CS.seek 0ml @13:23:36.13 delay 7 seconds @13:23:43.16 CS.seek 3ml @13:23:51.16 CSV.dial :waste @13:23:51.88 CS.seek 0ml @13:23:59.60 Garaging FlushPuck in Collection Clamp @13:23:59.67 Elbow.reconfigure :tight @13:23:59.72 Elbow.seek CC @13:24:00.08 Elbow.reconfigure :loose @13:24:00.18 Forearm.seek CC @13:24:01.24 Hand.close @13:24:01.90 CC.seek :open @13:24:06.93 Forearm.seek :retract @13:24:07.96 Elbow.reconfigure :tight @13:24:08.01 Elbow.seek :garage @13:24:11.05 Elbow.reconfigure :loose @13:24:11.15 Forearm.reconfigure :tight @13:24:11.19 Forearm.seek :garage @13:24:12.22 Hand.open @13:24:12.84 Forearm.reconfigure :loose @13:24:12.88 Forearm.seek :retract @13:24:13.87 da sampling at most 1000ml, wcr at most 1000ml @13:24:13.91 DAfans.email "Started da in tube 5 at 13:24:13UTC04-Dec-23\n{1 => 33,\n 2 => 33,\n 3 => 31,\n 4 => 5,\n 5 => 28,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@13:19:55, 31% flow, 16.7C, 30% humidity, 15.1psia, 13.695V, 0.290A, 145.133Ah, 3.98W",Subject: "da sampling at most 1000ml, wcr at most 1000ml" @13:24:13.93
Loading sh1 puck from tube 5 into Collection Clamp @13:24:14.19 SC.reconfigure :tight @13:24:14.25 SC.seek 5 @13:24:17.78 SC.configure :limp @13:24:18.04 Elbow.reconfigure :tight @13:24:18.09 Elbow.seek 5 @13:24:18.31 upsync @13:24:20.01
Forearm.reconfigure :tight @13:24:20.06 Forearm.seek 5 + 450 counts @13:24:21.09 SE.reconfigure :pick @13:24:21.15 SE.seek :beamBreak @13:24:22.46 SE.configure :default @13:24:22.63 Elbow.seek 5 @13:24:22.83 Forearm.seek 5 + 300 counts @13:24:23.09 Forearm.reconfigure :loose @13:24:23.13 Hand.close @13:24:23.79 SE.seek :down @13:24:24.00
Forearm.seek :retract @13:24:24.79 SE.stop @13:24:24.96
Elbow.seek CC @13:24:26.53 Elbow.reconfigure :loose @13:24:26.63 Forearm.reconfigure :tight @13:24:26.68 Forearm.seek CC @13:24:27.92 CC.seek :closed @13:24:32.44 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.23 @13:24:32.45 Hand.open @13:24:33.08 Forearm.reconfigure :loose @13:24:33.13 Forearm.seek :retract @13:24:34.07 collectSample 1000ml w/bubble point of 24psi @13:24:34.09 Priming 7ml @13:24:34.10 Intake.select :close @13:24:34.11 Exhaust.select :close @13:24:34.12 SS.hold @13:24:34.32 Calibrating pressure and temperature sensors @13:24:34.35 CTV.dial :air @13:24:34.37 SSV.dial :spit,avoiding: :exhaust @13:24:34.44 SS.reconfigure :fast @13:24:34.49 SS.seek 5ml @13:24:37.35 SSV.dial CBV @13:24:37.37
CBV.dial SSV @13:24:38.70 delay 3.8 seconds @13:24:42.63 Sensor limits: Intake max 102.2psia; Outlet max 95.4psia @13:24:42.64 SSV.dial :spit @13:24:43.98 SS.seek 0ml @13:24:46.79 SSV.dial CBV @13:24:48.13 CTV.dial :intake @13:24:48.91 SSV.dial CBV @13:24:48.93
CBV.dial SSV @13:24:49.15 Allowing 40s for 7.0ml of remaining 7.0ml @13:24:49.17 Intake.select :open @13:24:51.34 Intake @14.4psia, vacuum from 8.9 to 11.9psi @13:24:51.35 SS.reconfigure :inhaling 2.20ml/s @13:24:51.40 SS.seek 7ml @13:24:58.42 Stroke completed -- waiting for pressure to equalize ... @13:24:58.43 SS.reconfigure :exhale @13:24:58.48 SS.hold @13:25:04.47 Intake.select :close @13:25:04.63 SSV.dial :exhaust @13:25:05.39 Exhaust.select :open @13:25:05.50 SS.seek 0ml @13:25:09.58 Exhaust.select :close @13:25:09.68 Sampling 1000.0ml @13:25:09.69 Intake.select :close @13:25:09.70 Exhaust.select :close @13:25:09.72 SS.reconfigure :default @13:25:09.76 SS.hold @13:25:09.96 CTV.dial :intake @13:25:10.04 SSV.dial CBV @13:25:10.06
CBV.dial SSV @13:25:10.86 Allowing 130s for 25.0ml of remaining 1000.0ml @13:25:10.87 Intake.select :open @13:25:13.04 Intake @15.1psia, vacuum from 9.6 to 12.6psi @13:25:13.05 SS.reconfigure :inhaling 2.20ml/s @13:25:13.10 SS.seek 25ml @13:25:28.32 Stroke completed -- waiting for pressure to equalize ... @13:25:28.33 SS.reconfigure :exhale @13:25:28.38 SS.hold @13:25:57.07 Intake.select :close @13:25:57.23 SSV.dial :exhaust @13:25:58.00 Exhaust.select :open @13:25:58.11 SS.seek 0ml @13:26:06.90 Exhaust.select :close @13:26:07.02 SSV.dial CBV @13:26:07.06
CBV.dial SSV @13:26:07.79 Allowing 130s for 25.0ml of remaining 975.0ml @13:26:07.80 Intake.select :open @13:26:10.02 Intake @15.5psia, vacuum from 10.0 to 13.0psi @13:26:10.04 SS.reconfigure :inhaling 2.20ml/s @13:26:10.09 SS.seek 25ml @13:26:25.56 Stroke completed -- waiting for pressure to equalize ... @13:26:25.57 SS.reconfigure :exhale @13:26:25.61 SS.hold @13:27:00.40 Intake.select :close @13:27:00.55 SSV.dial :exhaust @13:27:01.31 Exhaust.select :open @13:27:01.42 SS.seek 0ml @13:27:10.25 Exhaust.select :close @13:27:10.35 SSV.dial CBV @13:27:10.39
CBV.dial SSV @13:27:11.14 Allowing 130s for 25.0ml of remaining 950.0ml @13:27:11.15 Intake.select :open @13:27:13.31 Intake @15.8psia, vacuum from 10.0 to 13.0psi @13:27:13.32 SS.reconfigure :inhaling 2.20ml/s @13:27:13.37 SS.seek 25ml @13:27:28.41 Vacuum too high -- waiting up to 40 seconds @13:27:28.42 SS.reconfigure :exhale @13:27:28.47 SS.hold @13:27:35.15 Intake @18.5psia, vacuum from 10.0 to 13.0psi @13:27:35.16 SS.reconfigure :inhaling 1.76ml/s @13:27:35.21 SS.seek 25ml @13:27:35.27 Stroke completed -- waiting for pressure to equalize ... @13:27:35.28 SS.reconfigure :exhale @13:27:35.32 SS.hold @13:28:01.86 Intake.select :close @13:28:02.02 SSV.dial :exhaust @13:28:02.77 Exhaust.select :open @13:28:02.88 SS.seek 0ml @13:28:11.71 Exhaust.select :close @13:28:11.82 SSV.dial CBV @13:28:11.85
CBV.dial SSV @13:28:13.14 Allowing 130s for 25.0ml of remaining 925.0ml @13:28:13.15 Intake.select :open @13:28:15.31 Intake @15.9psia, vacuum from 10.0 to 13.0psi @13:28:15.32 SS.reconfigure :inhaling 1.76ml/s @13:28:15.37 SS.seek 25ml @13:28:30.75 Vacuum too high -- waiting up to 40 seconds @13:28:30.77 SS.reconfigure :exhale @13:28:30.82 SS.hold @13:28:35.28 Intake @17.1psia, vacuum from 10.0 to 13.0psi @13:28:35.29 SS.reconfigure :inhaling 1.41ml/s @13:28:35.34 SS.seek 25ml @13:28:38.20 Stroke completed -- waiting for pressure to equalize ... @13:28:38.21 SS.reconfigure :exhale @13:28:38.25 SS.hold @13:29:00.78 Intake.select :close @13:29:00.94 SSV.dial :exhaust @13:29:01.70 Exhaust.select :open @13:29:01.81 SS.seek 0ml @13:29:10.81 Exhaust.select :close @13:29:10.92 SSV.dial CBV @13:29:10.94
CBV.dial SSV @13:29:11.73 Allowing 130s for 25.0ml of remaining 900.0ml @13:29:11.74 Intake.select :open @13:29:13.91 Intake @15.9psia, vacuum from 10.0 to 13.0psi @13:29:13.92 SS.reconfigure :inhaling 1.41ml/s @13:29:13.97 SS.seek 25ml @13:29:34.32 Stroke completed -- waiting for pressure to equalize ... @13:29:34.33 SS.reconfigure :exhale @13:29:34.38 SS.hold @13:29:59.19 Intake.select :close @13:29:59.34 SSV.dial :exhaust @13:30:00.10 Exhaust.select :open @13:30:00.22 SS.seek 0ml @13:30:09.02 Exhaust.select :close @13:30:09.13 SSV.dial CBV @13:30:09.15
CBV.dial SSV @13:30:09.94 Allowing 130s for 25.0ml of remaining 875.0ml @13:30:09.96 Intake.select :open @13:30:12.12 Intake @15.9psia, vacuum from 10.0 to 13.0psi @13:30:12.14 SS.reconfigure :inhaling 1.41ml/s @13:30:12.19 SS.seek 25ml @13:30:32.96 Stroke completed -- waiting for pressure to equalize ... @13:30:32.97 SS.reconfigure :exhale @13:30:33.02 SS.hold @13:30:53.31 Intake.select :close @13:30:53.46 SSV.dial :exhaust @13:30:54.24 Exhaust.select :open @13:30:54.35 SS.seek 0ml @13:31:03.10 Exhaust.select :close @13:31:03.21 SSV.dial CBV @13:31:03.23
CBV.dial SSV @13:31:04.02 Allowing 130s for 25.0ml of remaining 850.0ml @13:31:04.03 Intake.select :open @13:31:06.20 Intake @16.0psia, vacuum from 10.0 to 13.0psi @13:31:06.21 SS.reconfigure :inhaling 1.41ml/s @13:31:06.26 SS.seek 25ml @13:31:26.97 Stroke completed -- waiting for pressure to equalize ... @13:31:26.98 SS.reconfigure :exhale @13:31:27.03 SS.hold @13:31:47.42 Intake.select :close @13:31:47.57 SSV.dial :exhaust @13:31:48.34 Exhaust.select :open @13:31:48.46 SS.seek 0ml @13:31:57.52 Exhaust.select :close @13:31:57.63 SSV.dial CBV @13:31:57.66
CBV.dial SSV @13:31:58.44 Allowing 130s for 25.0ml of remaining 825.0ml @13:31:58.45 Intake.select :open @13:32:00.62 Intake @15.9psia, vacuum from 10.0 to 13.0psi @13:32:00.64 SS.reconfigure :inhaling 1.41ml/s @13:32:00.69 SS.seek 25ml @13:32:21.38 Stroke completed -- waiting for pressure to equalize ... @13:32:21.39 SS.reconfigure :exhale @13:32:21.44 SS.hold @13:32:39.72 Intake.select :close @13:32:39.87 SSV.dial :exhaust @13:32:40.63 Exhaust.select :open @13:32:40.74 SS.seek 0ml @13:32:49.50 Exhaust.select :close @13:32:49.61 SSV.dial CBV @13:32:49.63
CBV.dial SSV @13:32:50.41 Allowing 130s for 25.0ml of remaining 800.0ml @13:32:50.42 Intake.select :open @13:32:52.59 Intake @15.9psia, vacuum from 10.0 to 13.0psi @13:32:52.60 SS.reconfigure :inhaling 1.41ml/s @13:32:52.65 SS.seek 25ml @13:33:13.27 Stroke completed -- waiting for pressure to equalize ... @13:33:13.28 SS.reconfigure :exhale @13:33:13.68 SS.hold @13:33:30.04 Intake.select :close @13:33:30.20 SSV.dial :exhaust @13:33:30.96 Exhaust.select :open @13:33:31.07 SS.seek 0ml @13:33:39.80 Exhaust.select :close @13:33:39.91 SSV.dial CBV @13:33:39.93
CBV.dial SSV @13:33:40.71 Allowing 130s for 25.0ml of remaining 775.0ml @13:33:40.72 Intake.select :open @13:33:42.88 Intake @15.9psia, vacuum from 10.0 to 13.0psi @13:33:42.89 SS.reconfigure :inhaling 1.41ml/s @13:33:42.94 SS.seek 25ml @13:34:03.45 Stroke completed -- waiting for pressure to equalize ... @13:34:03.46 SS.reconfigure :exhale @13:34:03.51 SS.hold @13:34:18.02 Intake.select :close @13:34:18.18 SSV.dial :exhaust @13:34:18.94 Exhaust.select :open @13:34:19.05 SS.seek 0ml @13:34:27.74 Exhaust.select :close @13:34:27.84 SSV.dial CBV @13:34:27.86
CBV.dial SSV @13:34:28.64 Allowing 130s for 25.0ml of remaining 750.0ml @13:34:28.65 Intake.select :open @13:34:30.82 Intake @15.9psia, vacuum from 10.0 to 13.0psi @13:34:30.83 SS.reconfigure :inhaling 1.41ml/s @13:34:30.88 SS.seek 25ml @13:34:51.47 Stroke completed -- waiting for pressure to equalize ... @13:34:51.48 SS.reconfigure :exhale @13:34:51.53 SS.hold @13:35:06.28 Intake.select :close @13:35:06.43 SSV.dial :exhaust @13:35:07.19 Exhaust.select :open @13:35:07.30 SS.seek 0ml @13:35:16.11 Exhaust.select :close @13:35:16.22 SSV.dial CBV @13:35:16.24
CBV.dial SSV @13:35:17.02 Allowing 130s for 25.0ml of remaining 725.0ml @13:35:17.03 Intake.select :open @13:35:19.19 Intake @15.8psia, vacuum from 10.0 to 13.0psi @13:35:19.21 SS.reconfigure :inhaling 1.41ml/s @13:35:19.26 SS.seek 25ml @13:35:39.48 Stroke completed -- waiting for pressure to equalize ... @13:35:39.49 SS.reconfigure :exhale @13:35:39.54 SS.hold @13:35:52.55 Intake.select :close @13:35:52.78 SSV.dial :exhaust @13:35:53.55 Exhaust.select :open @13:35:53.66 SS.seek 0ml @13:36:02.59 Exhaust.select :close @13:36:02.70 SSV.dial CBV @13:36:02.73
CBV.dial SSV @13:36:03.50 Allowing 130s for 25.0ml of remaining 700.0ml @13:36:03.51 Intake.select :open @13:36:05.68 Intake @15.8psia, vacuum from 10.0 to 13.0psi @13:36:05.69 SS.reconfigure :inhaling 1.41ml/s @13:36:05.74 SS.seek 25ml @13:36:25.80 Stroke completed -- waiting for pressure to equalize ... @13:36:25.81 SS.reconfigure :exhale @13:36:25.85 SS.hold @13:36:40.25 Intake.select :close @13:36:40.40 SSV.dial :exhaust @13:36:41.16 Exhaust.select :open @13:36:41.27 SS.seek 0ml @13:36:50.26 Exhaust.select :close @13:36:50.37 SSV.dial CBV @13:36:50.41
CBV.dial SSV @13:36:51.16 Allowing 130s for 25.0ml of remaining 675.0ml @13:36:51.17 Intake.select :open @13:36:53.33 Intake @15.7psia, vacuum from 10.0 to 13.0psi @13:36:53.34 SS.reconfigure :inhaling 1.41ml/s @13:36:53.39 SS.seek 25ml @13:37:13.58 Stroke completed -- waiting for pressure to equalize ... @13:37:13.59 SS.reconfigure :exhale @13:37:13.64 SS.hold @13:37:26.30 Intake.select :close @13:37:26.46 SSV.dial :exhaust @13:37:27.22 Exhaust.select :open @13:37:27.33 SS.seek 0ml @13:37:36.11 Exhaust.select :close @13:37:36.22 SSV.dial CBV @13:37:36.24
CBV.dial SSV @13:37:37.01 Allowing 130s for 25.0ml of remaining 650.0ml @13:37:37.03 Intake.select :open @13:37:39.19 Intake @15.8psia, vacuum from 10.0 to 13.0psi @13:37:39.21 SS.reconfigure :inhaling 1.41ml/s @13:37:39.26 SS.seek 25ml @13:37:59.79 Stroke completed -- waiting for pressure to equalize ... @13:37:59.80 SS.reconfigure :exhale @13:37:59.85 SS.hold @13:38:12.93 Intake.select :close @13:38:13.09 SSV.dial :exhaust @13:38:13.84 Exhaust.select :open @13:38:13.96 SS.seek 0ml @13:38:22.56 Exhaust.select :close @13:38:22.67 SSV.dial CBV @13:38:22.69
CBV.dial SSV @13:38:23.47 Allowing 130s for 25.0ml of remaining 625.0ml @13:38:23.48 Intake.select :open @13:38:25.64 Intake @15.8psia, vacuum from 10.0 to 13.0psi @13:38:25.65 SS.reconfigure :inhaling 1.41ml/s @13:38:25.70 SS.seek 25ml @13:38:46.50 Stroke completed -- waiting for pressure to equalize ... @13:38:46.51 SS.reconfigure :exhale @13:38:46.55 SS.hold @13:38:59.33 Intake.select :close @13:38:59.49 SSV.dial :exhaust @13:39:00.25 Exhaust.select :open @13:39:00.36 SS.seek 0ml @13:39:09.11 Exhaust.select :close @13:39:09.21 SSV.dial CBV @13:39:09.23
CBV.dial SSV @13:39:10.03 Allowing 130s for 25.0ml of remaining 600.0ml @13:39:10.04 Intake.select :open @13:39:12.21 Intake @15.7psia, vacuum from 10.0 to 13.0psi @13:39:12.23 SS.reconfigure :inhaling 1.41ml/s @13:39:12.28 SS.seek 25ml @13:39:32.82 Stroke completed -- waiting for pressure to equalize ... @13:39:32.83 SS.reconfigure :exhale @13:39:32.88 SS.hold @13:39:47.24 Intake.select :close @13:39:47.40 SSV.dial :exhaust @13:39:48.17 Exhaust.select :open @13:39:48.28 SS.seek 0ml @13:39:57.14 Exhaust.select :close @13:39:57.25 SSV.dial CBV @13:39:57.29
CBV.dial SSV @13:39:58.03 Allowing 130s for 25.0ml of remaining 575.0ml @13:39:58.04 Intake.select :open @13:40:00.21 Intake @15.6psia, vacuum from 10.0 to 13.0psi @13:40:00.22 SS.reconfigure :inhaling 1.41ml/s @13:40:00.27 SS.seek 25ml @13:40:20.46 Stroke completed -- waiting for pressure to equalize ... @13:40:20.47 SS.reconfigure :exhale @13:40:20.52 SS.hold @13:40:34.90 Intake.select :close @13:40:35.05 SSV.dial :exhaust @13:40:35.81 Exhaust.select :open @13:40:35.92 SS.seek 0ml @13:40:44.54 Exhaust.select :close @13:40:44.65 SSV.dial CBV @13:40:44.67
CBV.dial SSV @13:40:45.47 Allowing 130s for 25.0ml of remaining 550.0ml @13:40:45.48 Intake.select :open @13:40:47.64 Intake @15.7psia, vacuum from 10.0 to 13.0psi @13:40:47.65 SS.reconfigure :inhaling 1.41ml/s @13:40:47.70 SS.seek 25ml @13:41:08.23 Stroke completed -- waiting for pressure to equalize ... @13:41:08.24 SS.reconfigure :exhale @13:41:08.28 SS.hold @13:41:22.93 Intake.select :close @13:41:23.09 SSV.dial :exhaust @13:41:23.85 Exhaust.select :open @13:41:23.97 SS.seek 0ml @13:41:32.46 Exhaust.select :close @13:41:32.58 SSV.dial CBV @13:41:32.61
CBV.dial SSV @13:41:33.35 Allowing 130s for 25.0ml of remaining 525.0ml @13:41:33.36 Intake.select :open @13:41:35.53 Intake @15.7psia, vacuum from 10.0 to 13.0psi @13:41:35.55 SS.reconfigure :inhaling 1.41ml/s @13:41:35.59 SS.seek 25ml @13:41:55.99 Stroke completed -- waiting for pressure to equalize ... @13:41:56.00 SS.reconfigure :exhale @13:41:56.05 SS.hold @13:42:08.81 Intake.select :close @13:42:08.96 SSV.dial :exhaust @13:42:09.72 Exhaust.select :open @13:42:09.83 SS.seek 0ml @13:42:18.46 Exhaust.select :close @13:42:18.57 SSV.dial CBV @13:42:18.59
CBV.dial SSV @13:42:19.38 Allowing 130s for 25.0ml of remaining 500.0ml @13:42:19.40 Intake.select :open @13:42:21.56 Intake @15.9psia, vacuum from 10.0 to 13.0psi @13:42:21.58 SS.reconfigure :inhaling 1.41ml/s @13:42:21.63 SS.seek 25ml @13:42:42.24 Stroke completed -- waiting for pressure to equalize ... @13:42:42.25 SS.reconfigure :exhale @13:42:42.30 SS.hold @13:42:56.70 Intake.select :close @13:42:56.86 SSV.dial :exhaust @13:42:57.62 Exhaust.select :open @13:42:57.74 SS.seek 0ml @13:43:06.43 Exhaust.select :close @13:43:06.54 SSV.dial CBV @13:43:06.57
CBV.dial SSV @13:43:07.34 Allowing 130s for 25.0ml of remaining 475.0ml @13:43:07.35 Intake.select :open @13:43:09.51 Intake @15.9psia, vacuum from 10.0 to 13.0psi @13:43:09.52 SS.reconfigure :inhaling 1.41ml/s @13:43:09.58 SS.seek 25ml @13:43:30.22 Stroke completed -- waiting for pressure to equalize ... @13:43:30.24 SS.reconfigure :exhale @13:43:30.28 SS.hold @13:43:44.66 Intake.select :close @13:43:44.82 SSV.dial :exhaust @13:43:45.59 Exhaust.select :open @13:43:45.70 SS.seek 0ml @13:43:54.40 Exhaust.select :close @13:43:54.50 SSV.dial CBV @13:43:54.53
CBV.dial SSV @13:43:55.30 Allowing 130s for 25.0ml of remaining 450.0ml @13:43:55.32 Intake.select :open @13:43:57.48 Intake @15.8psia, vacuum from 10.0 to 13.0psi @13:43:57.49 SS.reconfigure :inhaling 1.41ml/s @13:43:57.54 SS.seek 25ml @13:44:18.08 Stroke completed -- waiting for pressure to equalize ... @13:44:18.09 SS.reconfigure :exhale @13:44:18.14 SS.hold @13:44:34.45 Intake.select :close @13:44:34.61 SSV.dial :exhaust @13:44:35.37 Exhaust.select :open @13:44:35.48 SS.seek 0ml @13:44:44.39 Exhaust.select :close @13:44:44.56 SSV.dial CBV @13:44:44.58
CBV.dial SSV @13:44:45.37 Allowing 130s for 25.0ml of remaining 425.0ml @13:44:45.38 Intake.select :open @13:44:47.55 Intake @15.7psia, vacuum from 10.0 to 13.0psi @13:44:47.56 SS.reconfigure :inhaling 1.41ml/s @13:44:47.61 SS.seek 25ml @13:45:08.10 Stroke completed -- waiting for pressure to equalize ... @13:45:08.11 SS.reconfigure :exhale @13:45:08.16 SS.hold @13:45:24.45 Intake.select :close @13:45:24.61 SSV.dial :exhaust @13:45:25.38 Exhaust.select :open @13:45:25.49 SS.seek 0ml @13:45:34.24 Exhaust.select :close @13:45:34.35 SSV.dial CBV @13:45:34.37
CBV.dial SSV @13:45:35.13 Allowing 130s for 25.0ml of remaining 400.0ml @13:45:35.14 Intake.select :open @13:45:37.31 Intake @15.8psia, vacuum from 10.0 to 13.0psi @13:45:37.32 SS.reconfigure :inhaling 1.41ml/s @13:45:37.37 SS.seek 25ml @13:45:58.09 Stroke completed -- waiting for pressure to equalize ... @13:45:58.10 SS.reconfigure :exhale @13:45:58.14 SS.hold @13:46:16.51 Intake.select :close @13:46:16.66 SSV.dial :exhaust @13:46:17.43 Exhaust.select :open @13:46:17.54 SS.seek 0ml @13:46:26.29 Exhaust.select :close @13:46:26.40 SSV.dial CBV @13:46:26.43
CBV.dial SSV @13:46:27.18 Allowing 130s for 25.0ml of remaining 375.0ml @13:46:27.19 Intake.select :open @13:46:29.35 Intake @15.8psia, vacuum from 10.0 to 13.0psi @13:46:29.36 SS.reconfigure :inhaling 1.41ml/s @13:46:29.41 SS.seek 25ml @13:46:49.95 Stroke completed -- waiting for pressure to equalize ... @13:46:49.97 SS.reconfigure :exhale @13:46:50.01 SS.hold @13:47:08.63 Intake.select :close @13:47:08.79 SSV.dial :exhaust @13:47:09.55 Exhaust.select :open @13:47:09.67 SS.seek 0ml @13:47:18.30 Exhaust.select :close @13:47:18.41 SSV.dial CBV @13:47:18.43
CBV.dial SSV @13:47:19.22 Allowing 130s for 25.0ml of remaining 350.0ml @13:47:19.23 Intake.select :open @13:47:21.40 Intake @15.8psia, vacuum from 10.0 to 13.0psi @13:47:21.41 SS.reconfigure :inhaling 1.41ml/s @13:47:21.46 SS.seek 25ml @13:47:41.94 Stroke completed -- waiting for pressure to equalize ... @13:47:41.95 SS.reconfigure :exhale @13:47:42.00 SS.hold @13:48:00.67 Intake.select :close @13:48:00.82 SSV.dial :exhaust @13:48:01.58 Exhaust.select :open @13:48:01.70 SS.seek 0ml @13:48:10.58 Exhaust.select :close @13:48:10.69 SSV.dial CBV @13:48:10.71
CBV.dial SSV @13:48:11.50 Allowing 130s for 25.0ml of remaining 325.0ml @13:48:11.51 Intake.select :open @13:48:13.68 Intake @15.9psia, vacuum from 10.0 to 13.0psi @13:48:13.69 SS.reconfigure :inhaling 1.41ml/s @13:48:13.74 SS.seek 25ml @13:48:33.84 Stroke completed -- waiting for pressure to equalize ... @13:48:33.85 SS.reconfigure :exhale @13:48:33.90 SS.hold @13:48:55.00 Intake.select :close @13:48:55.15 SSV.dial :exhaust @13:48:55.91 Exhaust.select :open @13:48:56.02 SS.seek 0ml @13:49:04.95 Exhaust.select :close @13:49:05.06 SSV.dial CBV @13:49:05.09
CBV.dial SSV @13:49:05.86 Allowing 130s for 25.0ml of remaining 300.0ml @13:49:05.87 Intake.select :open @13:49:08.04 Intake @15.7psia, vacuum from 10.0 to 13.0psi @13:49:08.05 SS.reconfigure :inhaling 1.41ml/s @13:49:08.10 SS.seek 25ml @13:49:28.53 Stroke completed -- waiting for pressure to equalize ... @13:49:28.54 SS.reconfigure :exhale @13:49:28.59 SS.hold @13:49:49.06 Intake.select :close @13:49:49.22 SSV.dial :exhaust @13:49:49.99 Exhaust.select :open @13:49:50.10 SS.seek 0ml @13:49:58.98 Exhaust.select :close @13:49:59.09 SSV.dial CBV @13:49:59.12
CBV.dial SSV @13:49:59.89 Allowing 130s for 25.0ml of remaining 275.0ml @13:49:59.91 Intake.select :open @13:50:02.07 Intake @15.9psia, vacuum from 10.0 to 13.0psi @13:50:02.08 SS.reconfigure :inhaling 1.41ml/s @13:50:02.13 SS.seek 25ml @13:50:22.29 Stroke completed -- waiting for pressure to equalize ... @13:50:22.30 SS.reconfigure :exhale @13:50:22.35 SS.hold @13:50:43.18 Intake.select :close @13:50:43.33 SSV.dial :exhaust @13:50:44.10 Exhaust.select :open @13:50:44.22 SS.seek 0ml @13:50:53.01 Exhaust.select :close @13:50:53.12 SSV.dial CBV @13:50:53.15
CBV.dial SSV @13:50:53.92 Allowing 130s for 25.0ml of remaining 250.0ml @13:50:53.94 Intake.select :open @13:50:56.10 Intake @16.0psia, vacuum from 10.0 to 13.0psi @13:50:56.11 SS.reconfigure :inhaling 1.41ml/s @13:50:56.16 SS.seek 25ml @13:51:17.03 Stroke completed -- waiting for pressure to equalize ... @13:51:17.04 SS.reconfigure :exhale @13:51:17.09 SS.hold @13:51:39.45 Intake.select :close @13:51:39.60 SSV.dial :exhaust @13:51:40.37 Exhaust.select :open @13:51:40.49 SS.seek 0ml @13:51:49.08 Exhaust.select :close @13:51:49.19 SSV.dial CBV @13:51:49.23
CBV.dial SSV @13:51:49.97 Allowing 130s for 25.0ml of remaining 225.0ml @13:51:49.98 Intake.select :open @13:51:52.15 Intake @15.9psia, vacuum from 10.0 to 13.0psi @13:51:52.16 SS.reconfigure :inhaling 1.41ml/s @13:51:52.21 SS.seek 25ml @13:52:12.87 Stroke completed -- waiting for pressure to equalize ... @13:52:12.88 SS.reconfigure :exhale @13:52:12.93 SS.hold @13:52:35.48 Intake.select :close @13:52:35.63 SSV.dial :exhaust @13:52:36.39 Exhaust.select :open @13:52:36.50 SS.seek 0ml @13:52:45.26 Exhaust.select :close @13:52:45.38 SSV.dial CBV @13:52:45.42
CBV.dial SSV @13:52:46.15 Allowing 130s for 25.0ml of remaining 200.0ml @13:52:46.17 Intake.select :open @13:52:48.33 Intake @16.0psia, vacuum from 10.0 to 13.0psi @13:52:48.35 SS.reconfigure :inhaling 1.41ml/s @13:52:48.40 SS.seek 25ml @13:53:03.16 Vacuum too high -- waiting up to 40 seconds @13:53:03.17 SS.reconfigure :exhale @13:53:03.23 SS.hold @13:53:13.22 Intake @16.3psia, vacuum from 10.0 to 13.0psi @13:53:13.23 SS.reconfigure :inhaling 1.13ml/s @13:53:13.28 SS.seek 25ml @13:53:21.31 Stroke completed -- waiting for pressure to equalize ... @13:53:21.32 SS.reconfigure :exhale @13:53:21.72 SS.hold @13:53:36.02 Intake.select :close @13:53:36.18 SSV.dial :exhaust @13:53:36.95 Exhaust.select :open @13:53:37.06 SS.seek 0ml @13:53:46.03 Exhaust.select :close @13:53:46.13 SSV.dial CBV @13:53:46.15
CBV.dial SSV @13:53:46.95 Allowing 130s for 25.0ml of remaining 175.0ml @13:53:46.96 Intake.select :open @13:53:49.13 Intake @15.9psia, vacuum from 10.0 to 13.0psi @13:53:49.14 SS.reconfigure :inhaling 1.13ml/s @13:53:49.19 SS.seek 25ml @13:54:11.72 Vacuum too high -- waiting up to 40 seconds @13:54:11.74 SS.reconfigure :exhale @13:54:11.79 SS.hold @13:54:23.46 Intake @17.1psia, vacuum from 10.0 to 13.0psi @13:54:23.47 SS.reconfigure :inhaling 0.91ml/s @13:54:23.52 SS.seek 25ml @13:54:26.18 Stroke completed -- waiting for pressure to equalize ... @13:54:26.19 SS.reconfigure :exhale @13:54:26.24 SS.hold @13:54:36.01 Intake.select :close @13:54:36.16 SSV.dial :exhaust @13:54:37.00 Exhaust.select :open @13:54:37.12 SS.seek 0ml @13:54:45.89 Exhaust.select :close @13:54:46.00 SSV.dial CBV @13:54:46.04
CBV.dial SSV @13:54:46.78 Allowing 130s for 25.0ml of remaining 150.0ml @13:54:46.79 Intake.select :open @13:54:48.95 Intake @15.9psia, vacuum from 10.0 to 13.0psi @13:54:48.96 SS.reconfigure :inhaling 0.91ml/s @13:54:49.01 SS.seek 25ml @13:55:18.91 Stroke completed -- waiting for pressure to equalize ... @13:55:18.92 SS.reconfigure :exhale @13:55:18.97 SS.hold @13:55:39.42 Intake.select :close @13:55:39.57 SSV.dial :exhaust @13:55:40.34 Exhaust.select :open @13:55:40.46 SS.seek 0ml @13:55:49.40 Exhaust.select :close @13:55:49.51 SSV.dial CBV @13:55:49.95
CBV.dial SSV @13:55:50.74 Allowing 130s for 25.0ml of remaining 125.0ml @13:55:50.76 Intake.select :open @13:55:52.92 Intake @15.9psia, vacuum from 10.0 to 13.0psi @13:55:52.94 SS.reconfigure :inhaling 0.91ml/s @13:55:52.99 SS.seek 25ml @13:56:22.63 Stroke completed -- waiting for pressure to equalize ... @13:56:22.64 SS.reconfigure :exhale @13:56:22.69 SS.hold @13:56:45.12 Intake.select :close @13:56:45.28 SSV.dial :exhaust @13:56:46.04 Exhaust.select :open @13:56:46.15 SS.seek 0ml @13:56:54.91 Exhaust.select :close @13:56:55.03 SSV.dial CBV @13:56:55.06
CBV.dial SSV @13:56:55.80 Allowing 130s for 25.0ml of remaining 100.0ml @13:56:56.17 Intake.select :open @13:56:58.41 Intake @15.9psia, vacuum from 10.0 to 13.0psi @13:56:58.42 SS.reconfigure :inhaling 0.91ml/s @13:56:58.47 SS.seek 25ml @13:57:28.37 Stroke completed -- waiting for pressure to equalize ... @13:57:28.38 SS.reconfigure :exhale @13:57:28.42 SS.hold @13:57:50.86 Intake.select :close @13:57:51.01 SSV.dial :exhaust @13:57:51.77 Exhaust.select :open @13:57:51.89 SS.seek 0ml @13:58:00.88 Exhaust.select :close @13:58:00.99 SSV.dial CBV @13:58:01.01
CBV.dial SSV @13:58:01.79 Allowing 130s for 25.0ml of remaining 75.0ml @13:58:01.80 Intake.select :open @13:58:03.97 Intake @15.9psia, vacuum from 10.0 to 13.0psi @13:58:03.98 SS.reconfigure :inhaling 0.91ml/s @13:58:04.46 SS.seek 25ml @13:58:34.13 Stroke completed -- waiting for pressure to equalize ... @13:58:34.14 SS.reconfigure :exhale @13:58:34.19 SS.hold @13:58:58.67 Intake.select :close @13:58:58.82 SSV.dial :exhaust @13:58:59.58 Exhaust.select :open @13:58:59.70 SS.seek 0ml @13:59:08.46 Exhaust.select :close @13:59:08.57 SSV.dial CBV @13:59:08.59
CBV.dial SSV @13:59:09.36 Allowing 130s for 25.0ml of remaining 50.0ml @13:59:09.38 Intake.select :open @13:59:11.54 Intake @16.0psia, vacuum from 10.0 to 13.0psi @13:59:11.56 SS.reconfigure :inhaling 0.91ml/s @13:59:12.03 SS.seek 25ml @13:59:38.43 Vacuum too high -- waiting up to 40 seconds @13:59:38.45 SS.reconfigure :exhale @13:59:38.50 SS.hold @13:59:51.31 Intake @16.9psia, vacuum from 10.0 to 13.0psi @13:59:51.32 SS.reconfigure :inhaling 0.73ml/s @13:59:51.37 SS.seek 25ml @13:59:55.83 Stroke completed -- waiting for pressure to equalize ... @13:59:55.84 SS.reconfigure :exhale @13:59:55.89 SS.hold @14:00:08.64 Intake.select :close @14:00:08.80 SSV.dial :exhaust @14:00:09.57 Exhaust.select :open @14:00:09.68 SS.seek 0ml @14:00:18.44 Exhaust.select :close @14:00:18.55 SSV.dial CBV @14:00:18.57
CBV.dial SSV @14:00:19.38 Allowing 130s for 24.9ml of remaining 24.9ml @14:00:19.39 Intake.select :open @14:00:21.56 Intake @16.0psia, vacuum from 10.0 to 13.0psi @14:00:21.57 SS.reconfigure :inhaling 0.73ml/s @14:00:21.62 SS.seek 24.95ml @14:00:57.78 Stroke completed -- waiting for pressure to equalize ... @14:00:57.79 SS.reconfigure :exhale @14:00:57.84 SS.hold @14:01:26.87 Intake.select :close @14:01:27.03 SSV.dial :exhaust @14:01:27.78 Exhaust.select :open @14:01:27.90 SS.seek 0ml @14:01:36.67 Exhaust.select :close @14:01:36.78 Sampled 1000.0ml @14:01:36.80 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @14:01:36.81 Intake.select :close @14:01:36.87 CSV.dial :waste @14:01:37.00 SSV.dial CBV,avoiding: :exhaust @14:01:37.02
CBV.dial SSV @14:01:37.14 CS.seek 0ml @14:01:37.22 CSV.dial :air @14:01:37.96 CS.seek 10ml @14:01:38.93
CTV.dial :air @14:01:39.72 delay 15 seconds @14:01:54.74 SSV.dial :spit,avoiding: :exhaust @14:01:56.05 CTV.dial CSV,avoiding: :intake @14:01:56.09
SS.seek 0ml @14:01:56.21 SSV.dial CBV,avoiding: :exhaust @14:02:02.56 SSV.dial between CBV and plug @14:02:03.07 CBV.dial between SSV and waste2 @14:02:03.46 CSV.dial between air and CTV @14:02:03.79 CS.seek 8.484ml @14:02:08.04 CTV.dial CSV,avoiding: :air @14:02:08.06
CSV.dial CTV @14:02:08.41 CS.reconfigure :pressurize @14:02:08.46 CS.seek 0ml @14:02:22.73 BubblePoint: Intake @24.2psia, Outlet @1.0psia @14:02:22.74 CBV.dial SSV @14:02:23.08 SS.reconfigure :default @14:02:23.56 SS.seek 10ml @14:02:29.03 delay 15 seconds @14:02:44.17 Evacuated: Intake @23.7psia, Outlet @23.8psia @14:02:44.18 SSV.dial :spit,avoiding: :exhaust @14:02:45.21 SS.reconfigure :exhale @14:02:45.26 SS.seek 0ml @14:02:50.22 SS.reconfigure :fast @14:02:50.27 SS.seek 5ml @14:02:53.06 SSV.dial CBV,avoiding: :exhaust @14:02:53.07
CBV.dial SSV @14:02:54.43 CTV.dial :air,avoiding: :intake @14:02:55.77 CSV.dial CTV @14:02:55.82
SS.seek 10ml @14:02:56.92 CSV.dial :air @14:02:57.38 CS.reconfigure :fast @14:02:57.43 CS.seek 0ml @14:02:58.32
SSV.dial :spit,avoiding: :exhaust @14:02:59.65 SS.reconfigure :exhale @14:02:59.70 SS.seek 0ml @14:03:04.68 SS.reconfigure :fast @14:03:04.73 SS.seek 5ml @14:03:05.43 CS.stop @14:03:07.63 SSV.dial CBV,avoiding: :exhaust @14:03:07.65
CBV.dial SSV @14:03:09.98 SSV.dial :spit,avoiding: :exhaust @14:03:11.29 SS.reconfigure :default @14:03:11.34 SS.seek 0ml @14:03:14.42 SS.stop @14:03:14.46 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @14:03:14.47 Intake.select :close @14:03:14.54 CSV.dial :waste @14:03:14.67 SSV.dial CBV,avoiding: :exhaust @14:03:14.69
CBV.dial SSV @14:03:15.31 CS.seek 0ml @14:03:15.37 CSV.dial :air @14:03:16.03
CTV.dial :air @14:03:16.13 delay 15 seconds @14:03:16.15 CS.seek 10ml @14:03:31.16
SSV.dial :spit,avoiding: :exhaust @14:03:32.48 CTV.dial CSV,avoiding: :intake @14:03:32.51
SS.reconfigure :exhale @14:03:32.60 SS.seek 0ml @14:03:33.02 SSV.dial CBV,avoiding: :exhaust @14:03:40.59 SSV.dial between CBV and plug @14:03:41.08 CBV.dial between SSV and waste2 @14:03:41.54 CSV.dial between air and CTV @14:03:41.86 CS.seek 7.835ml @14:03:47.63 CTV.dial CSV,avoiding: :air @14:03:47.65
CSV.dial CTV @14:03:48.03 CS.reconfigure :pressurize @14:03:48.09 CS.seek 0ml @14:04:02.94 BubblePoint: Intake @24.4psia, Outlet @2.9psia @14:04:02.95 CBV.dial SSV @14:04:03.30 SS.reconfigure :default @14:04:03.35 SS.seek 10ml @14:04:08.91 delay 15 seconds @14:04:24.04 Evacuated: Intake @24.0psia, Outlet @24.3psia @14:04:24.05 SSV.dial :spit,avoiding: :exhaust @14:04:25.10 SS.reconfigure :exhale @14:04:25.15 SS.seek 0ml @14:04:30.06 SS.reconfigure :fast @14:04:30.11 SS.seek 5ml @14:04:32.91 SSV.dial CBV,avoiding: :exhaust @14:04:32.93
CBV.dial SSV @14:04:34.30 CTV.dial :air,avoiding: :intake @14:04:35.62 CSV.dial CTV @14:04:35.68
SS.seek 10ml @14:04:36.83 CSV.dial :air @14:04:37.39 CS.reconfigure :fast @14:04:37.44 CS.seek 0ml @14:04:38.14
SSV.dial :spit,avoiding: :exhaust @14:04:39.46 SS.reconfigure :exhale @14:04:39.51 SS.seek 0ml @14:04:43.40 CS.stop @14:04:44.60
SS.reconfigure :fast @14:04:44.65 SS.seek 5ml @14:04:47.37 SSV.dial CBV,avoiding: :exhaust @14:04:47.39
CBV.dial SSV @14:04:49.74 SSV.dial :spit,avoiding: :exhaust @14:04:51.05 SS.reconfigure :default @14:04:51.10 SS.seek 0ml @14:04:54.33 SS.stop @14:04:54.37 Lysing: Adding 2.0ml AqMeOH @14:04:54.38 CBV.dial between CSV and waste1,avoiding: :DBV @14:04:55.89 CTV.dial CSV @14:04:57.20 CSV.dial CSR @14:04:57.98 Pulling 1ml of CSR.AqMeOH CSR.select :AqMeOH @14:04:58.10 CS.reconfigure :default @14:04:58.14 CS.seek 1ml @14:05:01.56 delay 5 seconds @14:05:06.58 CSR.select :air @14:05:06.75 CS.seek 6ml @14:05:20.37 delay 2 seconds @14:05:22.75 CSV.dial :waste2 @14:05:23.38 CS.reconfigure :fast @14:05:23.43 CS.seek 0ml @14:05:37.71 CSV.dial CSR @14:05:38.15 Pulling 2ml of CSR.AqMeOH @14:05:38.16 CSR.select :AqMeOH @14:05:38.27 CS.reconfigure :default @14:05:38.32 CS.seek 2ml @14:05:44.50 delay 5 seconds @14:05:49.51 CSR.select :air @14:05:49.68 CS.seek 5ml @14:05:58.28 delay 2 seconds @14:06:00.29 CSV.dial CTV @14:06:00.74 CS.seek 1ml @14:06:11.98 Before lysing Collection Puck Heater at 12.5C/26.6psia @14:06:12.00 Heating Puck.from 5, :sh1 in Collection Clamp to 70C for 10:00 @14:06:12.03 CH.seek 70C,8:00 @14:08:28.21 CH.hold 10:00,70C @14:08:28.41 Begun lysing Collection Puck Heater at 68.1C/33.5psia @14:18:28.43 Ended lysing Collection Puck Heater at 70C/32.5psia @14:18:28.45 CS.seek 0ml @14:18:31.83 CTV.dial between spare and air,avoiding: :intake @14:18:32.89 CSV.dial :air @14:18:33.36 CS.seek 5ml @14:18:47.14 CSV.dial CTV @14:18:47.59 delay 2 seconds @14:18:49.61 CSV.dial :air @14:18:50.06 delay 2 seconds @14:18:52.08 CSV.dial CTV @14:18:52.54 delay 2 seconds @14:18:54.55 CSV.dial :air @14:18:55.00 delay 2 seconds @14:18:57.02 CS.seek 0ml @14:19:10.75 Puck @64.5psia after final lysis pressurization @14:19:10.77 Recovering Lysate into Collection syringe @14:19:10.78 CSV.dial CBV @14:19:11.80 CBV.dial CSV @14:19:12.19 CS.seek 5ml @14:19:26.01 delay 5 seconds @14:19:31.03 CTV.dial :air @14:19:31.64 CS.seek 6.999ml @14:19:37.51 delay 5 seconds @14:19:42.53 CSV.dial :air2 @14:19:42.97 CS.seek 10ml @14:19:51.45 CSV.dial between air and CTV,avoiding: CBV @14:19:52.61 Lysing Complete @14:19:52.68 Storing sh1 puck in Collection Clamp to tube 4 @14:19:52.86 SC.reconfigure :tight @14:19:52.90 SC.seek 4 @14:19:56.45 SC.configure :limp @14:19:56.66 Elbow.reconfigure :tight @14:19:56.71 Elbow.seek 4 @14:19:58.49 Forearm.reconfigure :tight @14:19:58.54 Forearm.seek 4 + 450 counts @14:19:59.56 SE.reconfigure :pick @14:19:59.61 SE.seek :beamBreak @14:20:02.46 SE.configure :default @14:20:02.62 Elbow.reconfigure :limp @14:20:02.67 Forearm.reconfigure :loose @14:20:02.71 Forearm.seek 4 @14:20:02.99 Elbow.hold @14:20:03.07 Elbow.reconfigure :tight @14:20:03.17 SE.seek 4.18 Pucks below Top Plate @14:20:03.60 Forearm.force -20 @14:20:03.64 Forearm.seek 4 + 282 counts @14:20:03.92 Forearm.reconfigure :tight @14:20:03.97 Forearm.seek 4 + 450 counts @14:20:04.22 SE.seek 5.18 Pucks below Top Plate @14:20:04.84 Forearm.reconfigure :loose @14:20:04.89 Forearm.seek :retract @14:20:05.83 Elbow.seek CC @14:20:07.96 Elbow.reconfigure :loose @14:20:08.09 Forearm.seek CC @14:20:09.07 Hand.close @14:20:09.73 CC.seek :open @14:20:14.47 Forearm.seek :retract @14:20:15.42 Elbow.reconfigure :tight @14:20:15.47 Elbow.seek 4 @14:20:17.25 Elbow.reconfigure :limp @14:20:17.30 Forearm.seek 4 @14:20:18.26 Elbow.hold @14:20:18.33 Elbow.reconfigure :tight @14:20:18.37 Hand.open @14:20:19.11 Forearm.force -20 @14:20:19.17 Forearm.seek 4 + 308 counts @14:20:19.49 Forearm.reconfigure :tight @14:20:19.54 Forearm.seek 4 + 450 counts @14:20:19.80 SE.seek :down @14:20:20.94
Forearm.reconfigure :loose @14:20:20.99 Forearm.seek :retract @14:20:22.14 SE.stop @14:20:22.19
Tranferring DA @14:20:22.20 Beginning samplePassC @14:20:22.21 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:20:22.40 Elbow.seek FlushPuck::Garage @14:20:24.24 Elbow.reconfigure :loose @14:20:24.34 Forearm.seek :garage @14:20:25.29 Hand.close @14:20:25.97 Forearm.seek :retract @14:20:27.36 Elbow.reconfigure :tight @14:20:27.41 Elbow.seek CC @14:20:30.38 Elbow.reconfigure :loose @14:20:30.48 Forearm.reconfigure :tight @14:20:30.53 Forearm.seek CC @14:20:31.78 CC.seek :closed @14:20:36.34 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.42 @14:20:36.42 Hand.open @14:20:37.04 Forearm.reconfigure :loose @14:20:37.09 Forearm.seek :retract @14:20:38.02 PSV.dial PSR @14:20:39.42 Pulling 1.5ml of PSR.flush PSR.select :flush @14:20:39.54 PS.seek 1.5ml @14:20:55.74 delay 5 seconds @14:21:00.76 PSV.dial :waste1 @14:21:01.24 PS.seek 0ml @14:21:17.58 PSV.dial PSR @14:21:18.05 PSR.select :air @14:21:18.17 PS.seek 2.5ml @14:21:44.46 PRV.dial CBV,avoiding: :ab2 @14:21:44.48
CBV.dial PRV @14:21:46.24 PSV.dial :mixloop @14:21:47.29 CTV.dial :air,avoiding: :intake @14:21:47.51 PTV.dial :PRVmixing @14:21:48.84 PS.seek 0ml @14:22:15.36 PSV.dial :air @14:22:15.82 PS.seek 2.5ml @14:22:42.17 PSV.dial :mixloop @14:22:43.09 PS.seek 0ml @14:23:09.48 PSV.dial :air @14:23:09.96 PS.seek 2.5ml @14:23:36.36 PSV.dial :mixloop @14:23:36.83 PS.seek 0ml @14:24:03.11 Garaging FlushPuck in Collection Clamp @14:24:03.18 Elbow.reconfigure :tight @14:24:03.23 Elbow.seek CC @14:24:03.80 Elbow.reconfigure :loose @14:24:03.90 Forearm.seek CC @14:24:04.91 Hand.close @14:24:05.48 CC.seek :open @14:24:10.10 Forearm.seek :retract @14:24:11.07 Elbow.reconfigure :tight @14:24:11.12 Elbow.seek :garage @14:24:14.11 Elbow.reconfigure :loose @14:24:14.21 Forearm.reconfigure :tight @14:24:14.26 Forearm.seek :garage @14:24:15.31 Hand.open @14:24:16.04 Forearm.reconfigure :loose @14:24:16.08 Forearm.seek :retract @14:24:17.13 PSV.dial :mixloop @14:24:17.20 PTV.dial :PRVmixing @14:24:17.22 PRV.dial CBV @14:24:17.24
CBV.dial PRV @14:24:17.48 CTV.dial :air,avoiding: :intake @14:24:17.50 Loading lyfil puck from tube 5 into Collection Clamp @14:24:17.73 SC.reconfigure :tight @14:24:17.78 SC.seek 5 @14:24:21.28 SC.configure :limp @14:24:21.50 Elbow.reconfigure :tight @14:24:21.55 Elbow.seek 5 @14:24:23.90 Forearm.reconfigure :tight @14:24:23.98 Forearm.seek 5 + 450 counts @14:24:25.00 SE.reconfigure :pick @14:24:25.06 SE.seek :beamBreak @14:24:26.45 SE.configure :default @14:24:26.61 Elbow.seek 5 @14:24:26.79 Forearm.seek 5 + 300 counts @14:24:27.01 Forearm.reconfigure :loose @14:24:27.06 Hand.close @14:24:27.74 SE.seek :down @14:24:27.95
Forearm.seek :retract @14:24:28.75 SE.stop @14:24:28.90
Elbow.seek CC @14:24:30.60 Elbow.reconfigure :loose @14:24:30.70 Forearm.reconfigure :tight @14:24:30.75 Forearm.seek CC @14:24:31.97 CC.seek :closed @14:24:36.49 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.27 @14:24:36.50 Hand.open @14:24:37.16 Forearm.reconfigure :loose @14:24:37.21 Forearm.seek :retract @14:24:38.18 CTV.dial CSV,avoiding: :intake @14:24:39.51 CSV.dial CTV @14:24:39.83 PS.seek 2.5ml @14:25:06.67 CS.seek 4.5ml @14:25:21.60 delay 8 seconds @14:25:29.62 PSV.dial :air @14:25:30.09 delay 2 seconds @14:25:32.11 PSV.dial :waste2 @14:25:32.85 PS.seek 0ml @14:25:59.26 CBV.dial between PRV and waste1 @14:25:59.59 CSV.dial :air @14:26:00.05 CS.seek 10ml @14:26:14.88 CSV.dial CTV @14:26:15.34 CS.seek 0ml @14:26:41.91 CBV.dial :waste1 @14:26:42.23 delay 5 seconds @14:26:47.25 CTV.dial :air,avoiding: :intake @14:26:48.66 Storing lyfil puck in Collection Clamp to tube 4 @14:26:48.84 SC.reconfigure :tight @14:26:48.89 SC.seek 4 @14:26:52.43 SC.configure :limp @14:26:52.64 Elbow.reconfigure :tight @14:26:52.69 Elbow.seek 4 @14:26:54.51 Forearm.reconfigure :tight @14:26:54.56 Forearm.seek 4 + 450 counts @14:26:55.57 SE.reconfigure :pick @14:26:55.62 SE.seek :beamBreak @14:26:58.41 SE.configure :default @14:26:58.57 Elbow.reconfigure :limp @14:26:58.62 Forearm.reconfigure :loose @14:26:58.66 Forearm.seek 4 @14:26:58.91 Elbow.hold @14:26:58.98 Elbow.reconfigure :tight @14:26:59.08 SE.seek 5.17 Pucks below Top Plate @14:26:59.52 Forearm.force -20 @14:26:59.93 Forearm.seek 4 + 286 counts @14:27:00.28 Forearm.reconfigure :tight @14:27:00.34 Forearm.seek 4 + 450 counts @14:27:00.59 SE.seek 6.17 Pucks below Top Plate @14:27:01.15 Forearm.reconfigure :loose @14:27:01.20 Forearm.seek :retract @14:27:02.10 Elbow.seek CC @14:27:03.76 Elbow.reconfigure :loose @14:27:03.86 Forearm.seek CC @14:27:04.89 Hand.close @14:27:05.48 CC.seek :open @14:27:10.44 Forearm.seek :retract @14:27:11.40 Elbow.reconfigure :tight @14:27:11.45 Elbow.seek 4 @14:27:13.20 Elbow.reconfigure :limp @14:27:13.25 Forearm.seek 4 @14:27:14.24 Elbow.hold @14:27:14.31 Elbow.reconfigure :tight @14:27:14.35 Hand.open @14:27:15.10 Forearm.force -20 @14:27:15.14 Forearm.seek 4 + 308 counts @14:27:15.55 Forearm.reconfigure :tight @14:27:15.60 Forearm.seek 4 + 450 counts @14:27:15.93 SE.seek :down @14:27:17.06
Forearm.reconfigure :loose @14:27:17.55 Forearm.seek :retract @14:27:18.28 SE.stop @14:27:18.51
PSV.dial :air @14:27:19.27 PS.seek 2.5ml @14:27:45.55 delay 1 second @14:27:46.56 PSV.dial :mixloop @14:27:47.01 delay 1 second @14:27:48.03 PSV.dial :air @14:27:48.50 delay 1 second @14:27:49.51 PSV.dial :mixloop @14:27:49.98 delay 1 second @14:27:51.00 PSV.dial :air @14:27:51.47 delay 1 second @14:27:52.49 PS.seek 0ml @14:28:18.76 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @14:28:18.95 Elbow.seek FlushPuck::Garage @14:28:20.80 Elbow.reconfigure :loose @14:28:20.90 Forearm.seek :garage @14:28:21.93 Hand.close @14:28:22.72 Forearm.seek :retract @14:28:23.71 Elbow.reconfigure :tight @14:28:23.76 Elbow.seek PC @14:28:25.05 Elbow.reconfigure :loose @14:28:25.15 Forearm.reconfigure :tight @14:28:25.20 Forearm.seek PC @14:28:26.41 PC.seek :closed @14:28:31.07 Processing Clamp onSpring/offSpring for 16/26 samples, on/off torque 1.65 @14:28:31.14 Hand.open @14:28:32.23 Forearm.reconfigure :loose @14:28:32.28 Forearm.seek :retract @14:28:33.22 PRV.dial :air @14:28:33.68 PSV.dial :mixloop @14:28:34.22 PS.seek 0.3ml @14:28:38.07 PSV.dial PSR @14:28:39.25 Pulling 0.4ml of PSR.air @14:28:39.26 PSR.select :air @14:28:39.28 PS.seek 0.7ml @14:28:44.13 delay 3 seconds @14:28:47.15 PSR.select :air @14:28:47.18 PS.seek 2.5ml @14:29:06.42 delay 3 seconds @14:29:09.43 PSV.dial :mixloop @14:29:10.59 PTV.dial :mixing @14:29:11.35 PBV.dial :waste2 @14:29:12.33 PS.seek 0ml @14:29:38.73 Garaging FlushPuck in Processing Clamp @14:29:38.80 Elbow.reconfigure :tight @14:29:38.85 Elbow.seek PC @14:29:38.97 Elbow.reconfigure :loose @14:29:39.07 Forearm.seek PC @14:29:40.02 Hand.close @14:29:40.60 PC.seek :open @14:29:45.34 Forearm.seek :retract @14:29:46.32 Elbow.reconfigure :tight @14:29:46.37 Elbow.seek :garage @14:29:47.73 Elbow.reconfigure :loose @14:29:47.83 Forearm.reconfigure :tight @14:29:47.88 Forearm.seek :garage @14:29:48.94 Hand.open @14:29:49.66 Forearm.reconfigure :loose @14:29:49.71 Forearm.seek :retract @14:29:50.75 Loading da puck from tube 5 into Processing Clamp @14:29:50.99 SC.reconfigure :tight @14:29:51.03 SC.seek 5 @14:29:54.56 SC.configure :limp @14:29:55.22 Elbow.reconfigure :tight @14:29:55.27 Elbow.seek 5 @14:29:57.14 Forearm.reconfigure :tight @14:29:57.19 Forearm.seek 5 + 450 counts @14:29:58.22 SE.reconfigure :pick @14:29:58.28 SE.seek :beamBreak @14:29:59.72 SE.configure :default @14:29:59.88 Elbow.seek 5 @14:30:00.08 Forearm.seek 5 + 300 counts @14:30:00.30 Forearm.reconfigure :loose @14:30:00.35 Hand.close @14:30:00.99 SE.seek :down @14:30:01.19
Forearm.seek :retract @14:30:02.05 SE.stop @14:30:02.16
Elbow.seek PC @14:30:04.70 Elbow.reconfigure :loose @14:30:04.80 Forearm.reconfigure :tight @14:30:04.85 Forearm.seek PC @14:30:06.12 PC.seek :closed @14:30:10.79 Processing Clamp onSpring/offSpring for 16/26 samples, on/off torque 1.65 @14:30:10.80 Hand.open @14:30:11.53 Forearm.reconfigure :loose @14:30:11.58 Forearm.seek :retract @14:30:12.55 Heating Puck.from 5, :da in Processing Clamp to 28C for 2:00:00 @14:30:12.57 PH.seek 28C,5:00 @14:30:55.30 PH.hold 2:00:00,28C @14:30:55.35 delay 1:30 @14:32:25.40 PSR.select :air @14:32:25.42 PSV.dial PSR @14:32:26.48 Pulling 0.5ml of PSR.wash PSR.select :wash @14:32:26.60 PS.seek 0.5ml @14:32:32.57 delay 5 seconds @14:32:37.59 PSR.select :air @14:32:37.71 PS.seek 2ml @14:32:53.91 PTV.dial :puck @14:32:54.65 PSV.dial PTV @14:32:55.41 PBV.dial :waste1 @14:32:56.80 PS.seek 1.5ml @14:33:02.79 delay 3 seconds @14:33:05.80 PBV.dial between PSV and waste1 @14:33:06.62 PS.seek 0ml @14:33:23.01 delay 5:00 @14:38:23.03 recoverPSRreagent08 @14:38:23.04 PTV.dial :puck @14:38:23.05 PBV.dial :waste1 @14:38:23.42 delay 30 seconds @14:38:53.45 PBV.dial between PSV and waste1 @14:38:53.83 PSR.select :air @14:38:53.84 PSV.dial PSR @14:38:54.61 PS.reconfigure :fast @14:38:54.66 PS.seek 2.5ml @14:39:18.53 PSV.dial PTV @14:39:19.30 delay 5 seconds @14:39:24.32 PSV.dial PSR @14:39:25.07 delay 5 seconds @14:39:30.09 PSV.dial PTV @14:39:30.84 delay 5 seconds @14:39:35.87 PSV.dial :air @14:39:36.63 PS.seek 0ml @14:39:59.87 PTV.dial :PRVmixing,via: PRV @14:40:01.22 PSV.dial :mixloop @14:40:01.69 PRV.dial :ab1 @14:40:02.44 PS.reconfigure :slow @14:40:02.49 PS.seek 0.15ml @14:40:06.22 delay 2 seconds @14:40:08.24 CBV.dial PRV @14:40:08.28
PRV.dial CBV @14:40:08.83 PS.seek 0.35ml @14:40:13.44 delay 2 seconds @14:40:15.45 CBV.dial between PRV and PSV @14:40:15.76 PRV.dial :air @14:40:16.22 PS.seek 0.9ml @14:40:28.00 PRV.dial :waste2 @14:40:28.75 PS.seek 0.5ml @14:40:37.50 addPMLReagent08 @14:40:37.51 PSV.dial :air @14:40:37.97 PS.reconfigure :default @14:40:38.02 PS.seek 2ml @14:40:54.21 PSV.dial :PML @14:40:54.68 PTV.dial :mixing @14:40:55.43 PBV.dial :waste1 @14:40:55.87 PS.seek 1.85ml @14:40:58.37 PBV.dial between PSV and waste1 @14:40:58.74 PS.seek 0ml @14:41:18.50 delay 2 seconds @14:41:20.52 Beginning samplePassC @14:41:20.53 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:41:20.71 Elbow.reconfigure :tight @14:41:21.20 Elbow.seek FlushPuck::Garage @14:41:22.57 Elbow.reconfigure :loose @14:41:22.67 Forearm.seek :garage @14:41:23.94 Hand.close @14:41:24.71 Forearm.seek :retract @14:41:25.69 Elbow.reconfigure :tight @14:41:25.73 Elbow.seek CC @14:41:28.66 Elbow.reconfigure :loose @14:41:28.76 Forearm.reconfigure :tight @14:41:28.80 Forearm.seek CC @14:41:30.06 CC.seek :closed @14:41:34.59 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.41 @14:41:34.65 Hand.open @14:41:35.28 Forearm.reconfigure :loose @14:41:35.32 Forearm.seek :retract @14:41:36.34 PSV.dial PSR @14:41:37.40 Pulling 1.5ml of PSR.flush @14:41:37.41 PSR.select :flush @14:41:37.53 PS.seek 1.5ml @14:41:53.65 delay 5 seconds @14:41:58.67 PSV.dial :waste1 @14:41:59.14 PS.seek 0ml @14:42:15.49 PSV.dial PSR @14:42:15.96 PSR.select :air @14:42:16.08 PS.seek 2.5ml @14:42:42.50 PRV.dial CBV,avoiding: :ab2 @14:42:42.52
CBV.dial PRV @14:42:44.54 PSV.dial :mixloop @14:42:45.65 CTV.dial :air,avoiding: :intake @14:42:45.73 PTV.dial :PRVmixing @14:42:46.50 PS.seek 0ml @14:43:12.87 PSV.dial :air @14:43:13.35 PS.seek 2.5ml @14:43:39.68 PSV.dial :mixloop @14:43:40.17 PS.seek 0ml @14:44:06.52 PSV.dial :air @14:44:07.02 PS.seek 2.5ml @14:44:33.52 PSV.dial :mixloop @14:44:34.00 PS.seek 0ml @14:45:00.31 Garaging FlushPuck in Collection Clamp @14:45:00.38 Elbow.reconfigure :tight @14:45:00.43 Elbow.seek CC @14:45:00.77 Elbow.reconfigure :loose @14:45:00.87 Forearm.seek CC @14:45:01.80 Hand.close @14:45:02.46 CC.seek :open @14:45:07.08 Forearm.seek :retract @14:45:08.03 Elbow.reconfigure :tight @14:45:08.07 Elbow.seek :garage @14:45:11.03 Elbow.reconfigure :loose @14:45:11.13 Forearm.reconfigure :tight @14:45:11.18 Forearm.seek :garage @14:45:12.23 Hand.open @14:45:12.90 Forearm.reconfigure :loose @14:45:12.94 Forearm.seek :retract @14:45:13.98 delayUntil "14:49:20.50" @14:45:14.01 Whole Cell Archive sampling 1000ml @14:45:14.02 Loading wcr puck from tube 5 into Collection Clamp @14:45:14.50 Elbow.reconfigure :tight @14:45:14.99 Elbow.seek 5 @14:45:17.06 Forearm.reconfigure :tight @14:45:17.12 Forearm.seek 5 + 450 counts @14:45:18.21 SE.reconfigure :pick @14:45:18.28 SE.seek :beamBreak @14:45:19.76 SE.configure :default @14:45:19.92 Elbow.seek 5 @14:45:20.16 Forearm.seek 5 + 300 counts @14:45:20.32 Forearm.reconfigure :loose @14:45:20.37 Hand.close @14:45:20.98 SE.seek :down @14:45:21.18 Forearm.seek :retract @14:45:22.12 SE.stop @14:45:22.18 Elbow.seek CC @14:45:23.88 Elbow.reconfigure :loose @14:45:23.99 Forearm.reconfigure :tight @14:45:24.04 Forearm.seek CC @14:45:25.24 CC.seek :closed @14:45:29.77 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.37 @14:45:29.78 Hand.open @14:45:30.85 Forearm.reconfigure :loose @14:45:30.90 Forearm.seek :retract @14:45:31.87 collectSample 1000ml w/bubble point of 35psi @14:45:31.88 Priming 7ml @14:45:31.89 Intake.select :close @14:45:31.91 Exhaust.select :close @14:45:31.92 SS.hold @14:45:32.13 Calibrating pressure sensors @14:45:32.14 CTV.dial :air @14:45:32.16 SSV.dial :spit,avoiding: :exhaust @14:45:32.24 SS.reconfigure :fast @14:45:32.30 SS.seek 5ml @14:45:34.96 SSV.dial CBV @14:45:35.00 CBV.dial SSV @14:45:36.33 delay 3.8 seconds @14:45:40.27 Sensor limits: Intake max 103.9psia; Outlet max 95.0psia @14:45:40.28 SSV.dial :spit @14:45:41.62 SS.seek 0ml @14:45:44.62 SSV.dial CBV @14:45:45.95 CTV.dial :intake @14:45:46.73 SSV.dial CBV @14:45:46.75 CBV.dial SSV @14:45:46.90 Allowing 40s for 7.0ml of remaining 7.0ml @14:45:46.91 Intake.select :open @14:45:49.08 Intake @14.4psia, vacuum from 8.9 to 11.9psi @14:45:49.10 SS.reconfigure :inhaling 2.20ml/s @14:45:49.15 SS.seek 7ml @14:45:55.43 Stroke completed -- waiting for pressure to equalize ... @14:45:55.45 SS.reconfigure :exhale @14:45:55.50 SS.hold @14:46:15.93 Intake.select :close @14:46:16.09 SSV.dial :exhaust @14:46:16.87 Exhaust.select :open @14:46:16.99 SS.seek 0ml @14:46:21.36 Exhaust.select :close @14:46:21.47 Sampling 1000.0ml @14:46:21.48 Intake.select :close @14:46:21.50 Exhaust.select :close @14:46:21.51 SS.reconfigure :default @14:46:21.55 SS.hold @14:46:21.76 CTV.dial :intake @14:46:21.95 SSV.dial CBV @14:46:21.97 CBV.dial SSV @14:46:22.75 Allowing 130s for 25.0ml of remaining 1000.0ml @14:46:22.77 Intake.select :open @14:46:24.93 Intake @15.5psia, vacuum from 10.0 to 13.0psi @14:46:24.95 SS.reconfigure :inhaling 2.20ml/s @14:46:24.99 SS.seek 25ml @14:46:31.79 Vacuum too high -- waiting up to 40 seconds @14:46:31.80 SS.reconfigure :exhale @14:46:31.86 SS.hold @14:46:39.96 Intake @18.6psia, vacuum from 10.0 to 13.0psi @14:46:39.98 SS.reconfigure :inhaling 1.76ml/s @14:46:40.02 SS.seek 25ml @14:46:42.45 Vacuum too high -- waiting up to 40 seconds @14:46:42.47 SS.reconfigure :exhale @14:46:42.52 SS.hold @14:46:44.76 Intake @18.6psia, vacuum from 10.0 to 13.0psi @14:46:44.78 SS.reconfigure :inhaling 1.41ml/s @14:46:44.83 SS.seek 25ml @14:46:47.13 Vacuum too high -- waiting up to 40 seconds @14:46:47.15 SS.reconfigure :exhale @14:46:47.20 SS.hold @14:46:49.60 Intake @19.0psia, vacuum from 10.0 to 13.0psi @14:46:49.62 SS.reconfigure :inhaling 1.13ml/s @14:46:49.66 SS.seek 25ml @14:46:52.10 Vacuum too high -- waiting up to 40 seconds @14:46:52.11 SS.reconfigure :exhale @14:46:52.17 SS.hold @14:46:54.31 Intake @19.1psia, vacuum from 10.0 to 13.0psi @14:46:54.33 SS.reconfigure :inhaling 0.91ml/s @14:46:54.37 SS.seek 25ml @14:46:57.51 Vacuum too high -- waiting up to 40 seconds @14:46:57.53 SS.reconfigure :exhale @14:46:57.58 SS.hold @14:46:59.71 Intake @19.0psia, vacuum from 10.0 to 13.0psi @14:46:59.72 SS.reconfigure :inhaling 0.73ml/s @14:46:59.77 SS.seek 25ml @14:47:04.45 Vacuum too high -- waiting up to 40 seconds @14:47:04.46 SS.reconfigure :exhale @14:47:04.51 SS.hold @14:47:06.45 SS.reconfigure :inhaling 0.59ml/s @14:47:06.50 SS.seek 25ml @14:47:12.21 Stroke completed -- waiting for pressure to equalize ... @14:47:12.23 SS.reconfigure :exhale @14:47:12.28 SS.hold @14:47:47.52 Intake.select :close @14:47:47.68 SSV.dial :exhaust @14:47:48.45 Exhaust.select :open @14:47:48.57 SS.seek 0ml @14:47:57.37 Exhaust.select :close @14:47:57.49 SSV.dial CBV @14:47:57.51 CBV.dial SSV @14:47:58.30 Allowing 130s for 25.0ml of remaining 975.0ml @14:47:58.31 Intake.select :open @14:48:00.48 Intake @15.6psia, vacuum from 10.0 to 13.0psi @14:48:00.50 SS.reconfigure :inhaling 0.59ml/s @14:48:00.55 SS.seek 25ml @14:48:45.45 Stroke completed -- waiting for pressure to equalize ... @14:48:45.46 SS.reconfigure :exhale @14:48:45.51 SS.hold @14:49:20.18 Intake.select :close @14:49:20.34 SSV.dial :exhaust @14:49:20.51 recoverPRVreagent08 @14:49:20.52 PTV.dial :mixing @14:49:21.11 Exhaust.select :open @14:49:21.22 SS.seek 0ml @14:49:21.30 PBV.dial :waste1 @14:49:21.68 delay 30 seconds @14:49:30.26 Exhaust.select :close @14:49:30.38 SSV.dial CBV @14:49:30.40 CBV.dial SSV @14:49:31.18 Allowing 130s for 25.0ml of remaining 950.0ml @14:49:31.20 Intake.select :open @14:49:33.37 Intake @15.6psia, vacuum from 10.0 to 13.0psi @14:49:33.39 SS.reconfigure :inhaling 0.59ml/s @14:49:33.43 SS.seek 25ml @14:49:51.70 PBV.dial between PSV and waste1 @14:49:52.07 PSV.dial :air @14:49:52.54 PS.reconfigure :fast @14:49:52.59 PS.seek 2.5ml @14:50:16.80 PSV.dial :mixloop @14:50:17.65 delay 2 seconds @14:50:17.98 Stroke completed -- waiting for pressure to equalize ... @14:50:17.99 SS.reconfigure :exhale @14:50:18.04 SS.hold @14:50:19.73 PSV.dial :air @14:50:20.21 delay 2 seconds @14:50:22.22 PSV.dial :mixloop @14:50:22.69 delay 2 seconds @14:50:24.71 PSV.dial :air @14:50:25.18 PS.seek 0ml @14:50:48.58 washCycleDA #1 of 2 @14:50:48.59 PSR.select :air @14:50:48.61 PSV.dial PSR @14:50:49.07 Intake.select :close @14:50:49.23 SSV.dial :exhaust @14:50:49.96 Pulling 0.5ml of PSR.wash @14:50:49.97 PSR.select :wash @14:50:50.03 Exhaust.select :open @14:50:50.11 PS.reconfigure :default @14:50:50.17 PS.seek 0.5ml @14:50:50.19 SS.seek 0ml @14:50:56.17 delay 5 seconds @14:50:59.02 Exhaust.select :close @14:50:59.13 SSV.dial CBV @14:50:59.17 CBV.dial SSV @14:50:59.93 Allowing 130s for 25.0ml of remaining 925.0ml @14:50:59.95 Intake.select :open @14:51:01.19 PSR.select :air @14:51:01.31 PS.seek 2ml @14:51:02.11 Intake @15.6psia, vacuum from 10.0 to 13.0psi @14:51:02.13 SS.reconfigure :inhaling 0.59ml/s @14:51:02.18 SS.seek 25ml @14:51:17.55 PTV.dial :puck @14:51:18.30 PSV.dial PTV @14:51:19.05 PBV.dial :waste1 @14:51:19.48 PS.seek 1.5ml @14:51:25.45 delay 3 seconds @14:51:28.47 PBV.dial between PSV and waste1 @14:51:28.85 PS.seek 0ml @14:51:45.02 delay 2:00 @14:51:47.21 Stroke completed -- waiting for pressure to equalize ... @14:51:47.22 SS.reconfigure :exhale @14:51:47.27 SS.hold @14:52:13.90 Intake.select :close @14:52:14.06 SSV.dial :exhaust @14:52:14.82 Exhaust.select :open @14:52:14.94 SS.seek 0ml @14:52:23.71 Exhaust.select :close @14:52:23.82 SSV.dial CBV @14:52:23.86 CBV.dial SSV @14:52:24.62 Allowing 130s for 25.0ml of remaining 900.0ml @14:52:24.64 Intake.select :open @14:52:26.81 Intake @15.6psia, vacuum from 10.0 to 13.0psi @14:52:26.83 SS.reconfigure :inhaling 0.59ml/s @14:52:26.87 SS.seek 25ml @14:53:11.58 Stroke completed -- waiting for pressure to equalize ... @14:53:11.60 SS.reconfigure :exhale @14:53:11.65 SS.hold @14:53:36.51 Intake.select :close @14:53:36.67 SSV.dial :exhaust @14:53:37.44 Exhaust.select :open @14:53:37.56 SS.seek 0ml @14:53:45.04 recoverPSRreagent08 @14:53:45.05 PTV.dial :puck @14:53:45.06 PBV.dial :waste1 @14:53:45.44 delay 30 seconds @14:53:46.54 Exhaust.select :close @14:53:46.66 SSV.dial CBV @14:53:46.68 CBV.dial SSV @14:53:47.46 Allowing 130s for 25.0ml of remaining 875.0ml @14:53:47.48 Intake.select :open @14:53:49.64 Intake @15.6psia, vacuum from 10.0 to 13.0psi @14:53:49.66 SS.reconfigure :inhaling 0.59ml/s @14:53:49.71 SS.seek 25ml @14:54:15.91 PBV.dial between PSV and waste1 @14:54:16.29 PSR.select :air @14:54:16.31 PSV.dial PSR @14:54:17.08 PS.reconfigure :fast @14:54:17.13 PS.seek 2.5ml @14:54:34.05 Stroke completed -- waiting for pressure to equalize ... @14:54:34.06 SS.reconfigure :exhale @14:54:34.11 SS.hold @14:54:41.07 PSV.dial PTV @14:54:41.86 delay 5 seconds @14:54:46.87 PSV.dial PSR @14:54:47.71 delay 5 seconds @14:54:52.73 PSV.dial PTV @14:54:53.50 delay 5 seconds @14:54:56.57 Intake.select :close @14:54:56.73 SSV.dial :exhaust @14:54:57.50 Exhaust.select :open @14:54:57.62 SS.seek 0ml @14:54:58.51 PSV.dial :air @14:54:59.41 PS.seek 0ml @14:55:06.50 Exhaust.select :close @14:55:06.61 SSV.dial CBV @14:55:06.63 CBV.dial SSV @14:55:07.41 Allowing 130s for 25.0ml of remaining 850.0ml @14:55:07.43 Intake.select :open @14:55:09.59 Intake @15.6psia, vacuum from 10.0 to 13.0psi @14:55:09.61 SS.reconfigure :inhaling 0.59ml/s @14:55:09.66 SS.seek 25ml @14:55:23.08 washCycleDA #2 of 2 @14:55:23.09 PSR.select :air @14:55:23.10 PSV.dial PSR @14:55:24.57 Pulling 0.5ml of PSR.wash @14:55:24.59 PSR.select :wash @14:55:24.70 PS.reconfigure :default @14:55:24.76 PS.seek 0.5ml @14:55:30.76 delay 5 seconds @14:55:35.78 PSR.select :air @14:55:35.90 PS.seek 2ml @14:55:52.13 PTV.dial :puck @14:55:52.15 PSV.dial PTV @14:55:52.90 PBV.dial :waste1 @14:55:53.34 PS.seek 1.5ml @14:55:54.61 Stroke completed -- waiting for pressure to equalize ... @14:55:54.62 SS.reconfigure :exhale @14:55:54.67 SS.hold @14:55:59.29 delay 3 seconds @14:56:02.31 PBV.dial between PSV and waste1 @14:56:02.67 PS.seek 0ml @14:56:15.23 Intake.select :close @14:56:15.39 SSV.dial :exhaust @14:56:16.16 Exhaust.select :open @14:56:16.28 SS.seek 0ml @14:56:19.01 delay 2:00 @14:56:25.16 Exhaust.select :close @14:56:25.27 SSV.dial CBV @14:56:25.30 CBV.dial SSV @14:56:26.94 Allowing 130s for 25.0ml of remaining 825.0ml @14:56:26.96 Intake.select :open @14:56:29.12 Intake @15.7psia, vacuum from 10.0 to 13.0psi @14:56:29.14 SS.reconfigure :inhaling 0.59ml/s @14:56:29.19 SS.seek 25ml @14:57:13.95 Stroke completed -- waiting for pressure to equalize ... @14:57:13.97 SS.reconfigure :exhale @14:57:14.01 SS.hold @14:57:34.81 Intake.select :close @14:57:34.97 SSV.dial :exhaust @14:57:35.76 Exhaust.select :open @14:57:35.88 SS.seek 0ml @14:57:44.89 Exhaust.select :close @14:57:45.00 SSV.dial CBV @14:57:45.04 CBV.dial SSV @14:57:45.82 Allowing 130s for 25.0ml of remaining 800.0ml @14:57:45.83 Intake.select :open @14:57:48.38 Intake @15.7psia, vacuum from 10.0 to 13.0psi @14:57:48.47 SS.reconfigure :inhaling 0.59ml/s @14:57:48.52 SS.seek 25ml @14:58:19.04 recoverPSRreagent08 @14:58:19.05 PTV.dial :puck @14:58:19.07 PBV.dial :waste1 @14:58:19.43 delay 30 seconds @14:58:33.15 Stroke completed -- waiting for pressure to equalize ... @14:58:33.17 SS.reconfigure :exhale @14:58:33.22 SS.hold @14:58:49.45 PBV.dial between PSV and waste1 @14:58:49.83 PSR.select :air @14:58:49.85 PSV.dial PSR @14:58:50.62 PS.reconfigure :fast @14:58:50.67 PS.seek 2.5ml @14:58:55.82 Intake.select :close @14:58:55.98 SSV.dial :exhaust @14:58:56.77 Exhaust.select :open @14:58:56.89 SS.seek 0ml @14:59:05.91 Exhaust.select :close @14:59:06.02 SSV.dial CBV @14:59:06.04 CBV.dial SSV @14:59:06.81 Allowing 130s for 25.0ml of remaining 775.0ml @14:59:06.83 Intake.select :open @14:59:09.00 Intake @15.5psia, vacuum from 10.0 to 13.0psi @14:59:09.02 SS.reconfigure :inhaling 0.59ml/s @14:59:09.06 SS.seek 25ml @14:59:14.66 PSV.dial PTV @14:59:15.43 delay 5 seconds @14:59:20.45 PSV.dial PSR @14:59:21.21 delay 5 seconds @14:59:26.23 PSV.dial PTV @14:59:27.01 delay 5 seconds @14:59:32.03 PSV.dial :air @14:59:32.80 PS.seek 0ml @14:59:53.54 Stroke completed -- waiting for pressure to equalize ... @14:59:53.56 SS.reconfigure :exhale @14:59:53.60 SS.hold @14:59:56.13 getPMLReagent08 @14:59:56.14 PRV.dial :air @14:59:56.62 delay 5 seconds @15:00:01.64 PTV.dial :PRVmixing @15:00:02.96 delay 5 seconds @15:00:08.02 PRV.dial :ab2 @15:00:08.49 PTV.dial :PRVmixing @15:00:08.57 PSV.dial :mixloop @15:00:09.05 PS.reconfigure :default @15:00:09.09 PS.seek 0.3ml @15:00:13.07 delay 2 seconds @15:00:14.04 Intake.select :close @15:00:14.20 SSV.dial :exhaust @15:00:14.98 Exhaust.select :open @15:00:15.08 PRV.dial :air @15:00:15.14 SS.seek 0ml @15:00:15.61 PS.seek 0.55ml @15:00:18.98 addPMLReagent08 @15:00:18.99 PSV.dial :air @15:00:19.47 PS.seek 2ml @15:00:23.95 Exhaust.select :close @15:00:24.07 SSV.dial CBV @15:00:24.11 CBV.dial SSV @15:00:24.86 Allowing 130s for 25.0ml of remaining 750.0ml @15:00:24.87 Intake.select :open @15:00:27.04 Intake @15.7psia, vacuum from 10.0 to 13.0psi @15:00:27.06 SS.reconfigure :inhaling 0.59ml/s @15:00:27.11 SS.seek 25ml @15:00:35.04 PSV.dial :PML @15:00:35.51 PTV.dial :mixing @15:00:36.27 PBV.dial :waste1 @15:00:36.71 PS.seek 1.85ml @15:00:39.19 PBV.dial between PSV and waste1 @15:00:39.58 PS.seek 0ml @15:00:59.44 delay 2 seconds @15:01:01.46 delay 8:00 @15:01:12.07 Stroke completed -- waiting for pressure to equalize ... @15:01:12.54 SS.reconfigure :exhale @15:01:12.59 SS.hold @15:01:35.07 Intake.select :close @15:01:35.22 SSV.dial :exhaust @15:01:35.98 Exhaust.select :open @15:01:36.10 SS.seek 0ml @15:01:45.56 Exhaust.select :close @15:01:45.74 SSV.dial CBV @15:01:45.77 CBV.dial SSV @15:01:46.55 Allowing 130s for 25.0ml of remaining 725.0ml @15:01:46.56 Intake.select :open @15:01:48.73 Intake @15.6psia, vacuum from 10.0 to 13.0psi @15:01:48.75 SS.reconfigure :inhaling 0.59ml/s @15:01:48.80 SS.seek 25ml @15:02:33.26 Stroke completed -- waiting for pressure to equalize ... @15:02:33.27 SS.reconfigure :exhale @15:02:33.32 SS.hold @15:02:58.29 Intake.select :close @15:02:58.45 SSV.dial :exhaust @15:02:59.23 Exhaust.select :open @15:02:59.35 SS.seek 0ml @15:03:08.28 Exhaust.select :close @15:03:08.39 SSV.dial CBV @15:03:08.41 CBV.dial SSV @15:03:09.18 Allowing 130s for 25.0ml of remaining 700.0ml @15:03:09.20 Intake.select :open @15:03:11.37 Intake @15.7psia, vacuum from 10.0 to 13.0psi @15:03:11.39 SS.reconfigure :inhaling 0.59ml/s @15:03:11.44 SS.seek 25ml @15:03:55.76 Stroke completed -- waiting for pressure to equalize ... @15:03:55.77 SS.reconfigure :exhale @15:03:55.82 SS.hold @15:04:20.36 Intake.select :close @15:04:20.52 SSV.dial :exhaust @15:04:21.92 Exhaust.select :open @15:04:22.03 SS.seek 0ml @15:04:30.88 Exhaust.select :close @15:04:30.99 SSV.dial CBV @15:04:31.02 CBV.dial SSV @15:04:31.81 Allowing 130s for 25.0ml of remaining 675.0ml @15:04:31.82 Intake.select :open @15:04:33.99 Intake @15.7psia, vacuum from 10.0 to 13.0psi @15:04:34.01 SS.reconfigure :inhaling 0.59ml/s @15:04:34.06 SS.seek 25ml @15:05:15.77 Vacuum too high -- waiting up to 40 seconds @15:05:15.78 SS.reconfigure :exhale @15:05:15.84 SS.hold @15:05:28.64 Intake @17.8psia, vacuum from 10.0 to 13.0psi @15:05:28.66 SS.reconfigure :inhaling 0.47ml/s @15:05:28.70 SS.seek 25ml @15:05:32.96 Stroke completed -- waiting for pressure to equalize ... @15:05:32.97 SS.reconfigure :exhale @15:05:33.02 SS.hold @15:05:49.36 Intake.select :close @15:05:49.52 SSV.dial :exhaust @15:05:50.30 Exhaust.select :open @15:05:50.42 SS.seek 0ml @15:05:59.24 Exhaust.select :close @15:05:59.35 SSV.dial CBV @15:05:59.40 CBV.dial SSV @15:06:00.15 Allowing 130s for 25.0ml of remaining 650.0ml @15:06:00.17 Intake.select :open @15:06:02.33 Intake @15.6psia, vacuum from 10.0 to 13.0psi @15:06:02.35 SS.reconfigure :inhaling 0.47ml/s @15:06:02.40 SS.seek 25ml @15:06:57.78 Stroke completed -- waiting for pressure to equalize ... @15:06:57.80 SS.reconfigure :exhale @15:06:57.85 SS.hold @15:07:20.35 Intake.select :close @15:07:20.52 SSV.dial :exhaust @15:07:21.30 Exhaust.select :open @15:07:21.42 SS.seek 0ml @15:07:30.46 Exhaust.select :close @15:07:30.57 SSV.dial CBV @15:07:30.59 CBV.dial SSV @15:07:31.38 Allowing 130s for 25.0ml of remaining 625.0ml @15:07:31.40 Intake.select :open @15:07:33.56 Intake @15.7psia, vacuum from 10.0 to 13.0psi @15:07:33.58 SS.reconfigure :inhaling 0.47ml/s @15:07:33.63 SS.seek 25ml @15:08:28.55 Stroke completed -- waiting for pressure to equalize ... @15:08:28.56 SS.reconfigure :exhale @15:08:28.61 SS.hold @15:08:53.63 Intake.select :close @15:08:53.79 SSV.dial :exhaust @15:08:54.55 Exhaust.select :open @15:08:54.67 SS.seek 0ml @15:09:01.48 recoverPRVreagent08 @15:09:01.49 PTV.dial :mixing @15:09:01.56 PBV.dial :waste1 @15:09:01.94 delay 30 seconds @15:09:03.75 Exhaust.select :close @15:09:03.86 SSV.dial CBV @15:09:03.88 CBV.dial SSV @15:09:04.66 Allowing 130s for 25.0ml of remaining 600.0ml @15:09:04.68 Intake.select :open @15:09:06.85 Intake @15.7psia, vacuum from 10.0 to 13.0psi @15:09:06.87 SS.reconfigure :inhaling 0.47ml/s @15:09:06.92 SS.seek 25ml @15:09:31.95 PBV.dial between PSV and waste1 @15:09:32.32 PSV.dial :air @15:09:32.80 PS.reconfigure :fast @15:09:32.85 PS.seek 2.5ml @15:09:57.03 PSV.dial :mixloop @15:09:57.51 delay 2 seconds @15:09:59.53 PSV.dial :air @15:10:00.12 delay 2 seconds @15:10:01.88 Stroke completed -- waiting for pressure to equalize ... @15:10:01.89 SS.reconfigure :exhale @15:10:01.94 SS.hold @15:10:02.14 PSV.dial :mixloop @15:10:03.05 delay 2 seconds @15:10:05.06 PSV.dial :air @15:10:05.66 PS.seek 0ml @15:10:29.05 washCycleDA #1 of 3 @15:10:29.06 Intake.select :close @15:10:29.08 PSR.select :air @15:10:29.19 PSV.dial PSR @15:10:29.29 SSV.dial :exhaust @15:10:30.54 Pulling 0.5ml of PSR.wash @15:10:30.56 Exhaust.select :open @15:10:30.57 PSR.select :wash @15:10:30.71 SS.seek 0ml @15:10:30.77 PS.reconfigure :default @15:10:30.82 PS.seek 0.5ml @15:10:36.81 delay 5 seconds @15:10:39.70 Exhaust.select :close @15:10:39.81 SSV.dial CBV @15:10:39.85 CBV.dial SSV @15:10:40.61 Allowing 130s for 25.0ml of remaining 575.0ml @15:10:40.63 Intake.select :open @15:10:41.82 PSR.select :air @15:10:41.95 PS.seek 2ml @15:10:42.80 Intake @15.6psia, vacuum from 10.0 to 13.0psi @15:10:42.82 SS.reconfigure :inhaling 0.47ml/s @15:10:42.86 SS.seek 25ml @15:10:58.06 PTV.dial :puck @15:10:58.82 PSV.dial PTV @15:10:59.59 PBV.dial :waste1 @15:11:00.03 PS.seek 1.5ml @15:11:05.93 delay 3 seconds @15:11:08.95 PBV.dial between PSV and waste1 @15:11:09.37 PS.seek 0ml @15:11:25.47 delay 2:00 @15:11:37.95 Stroke completed -- waiting for pressure to equalize ... @15:11:37.97 SS.reconfigure :exhale @15:11:38.01 SS.hold @15:12:06.64 Intake.select :close @15:12:06.80 SSV.dial :exhaust @15:12:07.56 Exhaust.select :open @15:12:07.68 SS.seek 0ml @15:12:16.67 Exhaust.select :close @15:12:16.79 SSV.dial CBV @15:12:16.81 CBV.dial SSV @15:12:17.58 Allowing 130s for 25.0ml of remaining 550.0ml @15:12:17.59 Intake.select :open @15:12:19.76 Intake @15.8psia, vacuum from 10.0 to 13.0psi @15:12:19.78 SS.reconfigure :inhaling 0.47ml/s @15:12:19.83 SS.seek 25ml @15:13:14.95 Stroke completed -- waiting for pressure to equalize ... @15:13:14.96 SS.reconfigure :exhale @15:13:15.01 SS.hold @15:13:25.49 recoverPSRreagent08 @15:13:25.50 PTV.dial :puck @15:13:25.68 PBV.dial :waste1 @15:13:26.06 delay 30 seconds @15:13:47.87 Intake.select :close @15:13:48.03 SSV.dial :exhaust @15:13:48.82 Exhaust.select :open @15:13:48.94 SS.seek 0ml @15:13:56.08 PBV.dial between PSV and waste1 @15:13:56.61 PSR.select :air @15:13:56.63 PSV.dial PSR @15:13:57.40 PS.reconfigure :fast @15:13:57.45 PS.seek 2.5ml @15:13:57.69 Exhaust.select :close @15:13:57.80 SSV.dial CBV @15:13:57.82 CBV.dial SSV @15:13:58.59 Allowing 130s for 25.0ml of remaining 525.0ml @15:13:58.61 Intake.select :open @15:14:00.78 Intake @15.7psia, vacuum from 10.0 to 13.0psi @15:14:00.79 SS.reconfigure :inhaling 0.47ml/s @15:14:00.84 SS.seek 25ml @15:14:21.49 PSV.dial PTV @15:14:22.27 delay 5 seconds @15:14:27.30 PSV.dial PSR @15:14:28.06 delay 5 seconds @15:14:33.08 PSV.dial PTV @15:14:33.84 delay 5 seconds @15:14:38.86 PSV.dial :air @15:14:39.64 PS.seek 0ml @15:14:56.84 Stroke completed -- waiting for pressure to equalize ... @15:14:56.85 SS.reconfigure :exhale @15:14:56.90 SS.hold @15:15:03.06 washCycleDA #2 of 3 @15:15:03.07 PSR.select :air @15:15:03.08 PSV.dial PSR @15:15:04.43 Pulling 0.5ml of PSR.wash @15:15:04.44 PSR.select :wash @15:15:04.55 PS.reconfigure :default @15:15:04.60 PS.seek 0.5ml @15:15:10.52 delay 5 seconds @15:15:15.54 PSR.select :air @15:15:15.71 PS.seek 2ml @15:15:31.96 PTV.dial :puck @15:15:32.04 PSV.dial PTV @15:15:32.20 Intake.select :close @15:15:32.36 SSV.dial :exhaust @15:15:32.79 PBV.dial :waste1 @15:15:33.12 Exhaust.select :open @15:15:33.26 PS.seek 1.5ml @15:15:33.32 SS.seek 0ml @15:15:39.21 delay 3 seconds @15:15:42.23 PBV.dial between PSV and waste1 @15:15:42.40 Exhaust.select :close @15:15:42.52 SSV.dial CBV @15:15:42.54 CBV.dial SSV @15:15:42.61 PS.seek 0ml @15:15:43.37 Allowing 130s for 25.0ml of remaining 500.0ml @15:15:43.39 Intake.select :open @15:15:45.56 Intake @15.8psia, vacuum from 10.0 to 13.0psi @15:15:45.58 SS.reconfigure :inhaling 0.47ml/s @15:15:45.62 SS.seek 25ml @15:15:58.87 delay 2:00 @15:16:40.95 Stroke completed -- waiting for pressure to equalize ... @15:16:40.96 SS.reconfigure :exhale @15:16:41.01 SS.hold @15:17:21.19 Intake.select :close @15:17:21.35 SSV.dial :exhaust @15:17:22.12 Exhaust.select :open @15:17:22.24 SS.seek 0ml @15:17:31.21 Exhaust.select :close @15:17:31.33 SSV.dial CBV @15:17:31.35 CBV.dial SSV @15:17:32.14 Allowing 130s for 25.0ml of remaining 475.0ml @15:17:32.15 Intake.select :open @15:17:34.32 Intake @15.8psia, vacuum from 10.0 to 13.0psi @15:17:34.34 SS.reconfigure :inhaling 0.47ml/s @15:17:34.39 SS.seek 25ml @15:17:58.89 recoverPSRreagent08 @15:17:58.90 PTV.dial :puck @15:17:58.97 PBV.dial :waste1 @15:17:59.33 delay 30 seconds @15:18:15.13 Vacuum too high -- waiting up to 40 seconds @15:18:15.14 SS.reconfigure :exhale @15:18:15.20 SS.hold @15:18:29.35 PBV.dial between PSV and waste1 @15:18:29.70 PSR.select :air @15:18:29.72 PSV.dial PSR @15:18:30.49 PS.reconfigure :fast @15:18:30.55 PS.seek 2.5ml @15:18:35.61 Intake @17.1psia, vacuum from 10.0 to 13.0psi @15:18:35.63 SS.reconfigure :inhaling 0.38ml/s @15:18:35.67 SS.seek 25ml @15:18:53.47 Stroke completed -- waiting for pressure to equalize ... @15:18:53.49 SS.reconfigure :exhale @15:18:53.54 SS.hold @15:18:54.64 PSV.dial PTV @15:18:55.41 delay 5 seconds @15:19:00.42 PSV.dial PSR @15:19:01.19 delay 5 seconds @15:19:06.27 PSV.dial PTV @15:19:07.05 delay 5 seconds @15:19:12.07 PSV.dial :air @15:19:12.84 PS.seek 0ml @15:19:18.12 Intake.select :close @15:19:18.28 SSV.dial :exhaust @15:19:19.05 Exhaust.select :open @15:19:19.17 SS.seek 0ml @15:19:28.18 Exhaust.select :close @15:19:28.29 SSV.dial CBV @15:19:28.31 CBV.dial SSV @15:19:29.10 Allowing 130s for 25.0ml of remaining 450.0ml @15:19:29.12 Intake.select :open @15:19:31.28 Intake @15.6psia, vacuum from 10.0 to 13.0psi @15:19:31.30 SS.reconfigure :inhaling 0.38ml/s @15:19:31.35 SS.seek 25ml @15:19:36.52 washCycleDA #3 of 3 @15:19:36.53 PSR.select :air @15:19:36.55 PSV.dial PSR @15:19:37.89 Pulling 0.5ml of PSR.wash @15:19:37.90 PSR.select :wash @15:19:38.02 PS.reconfigure :default @15:19:38.07 PS.seek 0.5ml @15:19:44.02 delay 5 seconds @15:19:49.04 PSR.select :air @15:19:49.16 PS.seek 2ml @15:20:05.86 PTV.dial :puck @15:20:05.94 PSV.dial PTV @15:20:06.69 PBV.dial :waste1 @15:20:07.12 PS.seek 1.5ml @15:20:13.05 delay 3 seconds @15:20:16.07 PBV.dial between PSV and waste1 @15:20:16.44 PS.seek 0ml @15:20:32.64 delay 2:00 @15:20:39.21 Stroke completed -- waiting for pressure to equalize ... @15:20:39.22 SS.reconfigure :exhale @15:20:39.27 SS.hold @15:21:19.46 SS.reconfigure :default @15:21:19.51 SS.hold @15:21:19.58 Intake.select :close @15:21:19.75 ShallowSampler::Clogged in WCR -- Intake @16psia & Outlet @14psia won't equalize after 575.0ml @15:21:19.76 SSV.dial :exhaust @15:21:20.52 Exhaust.select :open @15:21:20.63 SS.reconfigure :exhale @15:21:20.69 SS.seek 0ml @15:21:29.94 Exhaust.select :close @15:21:30.05 Sampled 575.0ml @15:21:30.07 Drying puck before fixing for 5:00 @15:21:30.08 CBV.dial :waste2b @15:21:30.85 delay 1:00 @15:22:30.88 CSV.dial :air @15:22:31.34 CS.reconfigure :fast @15:22:31.39 CS.seek 10ml @15:22:32.67 recoverPSRreagent08 @15:22:32.68 PTV.dial :puck @15:22:32.75 PBV.dial :waste1 @15:22:33.12 delay 30 seconds @15:22:56.24 Pumping wcr puck from tube 5 up to 35psi... @15:22:56.26 CBV.dial SSV @15:22:57.54 CBV.dial between CSV and waste2b @15:22:58.51 CSV.dial between air and CTV @15:22:58.84 CS.seek 7.278ml @15:23:03.14 PBV.dial between PSV and waste1 @15:23:03.51 PSR.select :air @15:23:03.53 PSV.dial PSR @15:23:04.30 PS.reconfigure :fast @15:23:04.83 PS.seek 2.5ml @15:23:05.96 CTV.dial CSV,avoiding: :air @15:23:05.99 CSV.dial CTV @15:23:06.84 CS.reconfigure :pressurize @15:23:06.89 CS.seek 0ml @15:23:20.13 CBV.dial :waste2b @15:23:20.48 CS.reconfigure :default @15:23:20.53 CS.seek 0ml @15:23:27.05 delay 1:00 @15:23:28.73 PSV.dial PTV @15:23:29.50 delay 5 seconds @15:23:34.52 PSV.dial PSR @15:23:35.26 delay 5 seconds @15:23:40.29 PSV.dial PTV @15:23:41.05 delay 5 seconds @15:23:46.07 PSV.dial :air @15:23:46.83 PS.seek 0ml @15:24:10.14 Gate.power :camera,:ON @15:24:10.15 Mixing DA Substrates @15:24:10.16 PSR.select :air @15:24:10.18 PSV.dial PSR @15:24:11.60 Pulling 0.25ml of PSR.sub1 @15:24:11.62 PSR.select :sub1 @15:24:11.83 PS.reconfigure :default @15:24:11.94 PS.seek 0.25ml @15:24:15.52 delay 5 seconds @15:24:20.54 Pulling 0.25ml of PSR.sub2 @15:24:20.56 PSR.select :sub2 @15:24:20.67 PS.seek 0.5ml @15:24:24.11 delay 5 seconds @15:24:27.07 CTV.dial :air,avoiding: :intake @15:24:28.46 Fix #1 of 2 (with RNAlater) for 2:30 @15:24:28.48 CSV.dial CSR @15:24:28.94 Pulling 2ml of CSR.RNAlater @15:24:28.95 CSR.select :RNAlater @15:24:29.07 CS.seek 2ml @15:24:29.12 PSR.select :air @15:24:29.24 PS.seek 2ml @15:24:35.20 delay 5 seconds @15:24:40.23 CSR.select :air @15:24:40.34 CS.reconfigure :fast @15:24:40.40 CS.seek 10ml @15:24:45.39 PTV.dial :puck @15:24:45.46 PSV.dial PTV @15:24:46.23 PBV.dial :waste1 @15:24:46.67 PS.seek 1.5ml @15:24:52.57 delay 3 seconds @15:24:55.59 PBV.dial between PSV and waste1 @15:24:55.97 PS.seek 0ml @15:25:00.46 Pumping wcr puck from tube 5 up to 35psi... @15:25:00.48 CBV.dial SSV @15:25:01.74 CBV.dial between CSV and waste2b @15:25:03.18 CSV.dial between air and CTV @15:25:03.84 CS.seek 7.195ml @15:25:11.21 CTV.dial CSV,avoiding: :air @15:25:11.23 CSV.dial CTV @15:25:12.13 delay 10 seconds @15:25:12.65 CS.reconfigure :pressurize @15:25:12.70 CS.seek 0ml @15:25:22.16 recoverPSRreagent08 @15:25:22.17 PTV.dial :puck @15:25:22.24 PBV.dial :waste1 @15:25:22.62 delay 30 seconds @15:25:31.07 delay 2:30 @15:25:52.64 PBV.dial between PSV and waste1 @15:25:53.01 PSR.select :air @15:25:53.03 PSV.dial PSR @15:25:53.82 PS.reconfigure :fast @15:25:53.87 PS.seek 2.5ml @15:26:18.01 PSV.dial PTV @15:26:18.79 delay 5 seconds @15:26:23.81 PSV.dial PSR @15:26:24.61 delay 5 seconds @15:26:29.63 PSV.dial PTV @15:26:30.40 delay 5 seconds @15:26:35.42 PSV.dial :air @15:26:36.20 PS.seek 0ml @15:27:00.01 Additional air clear @15:27:00.02 PSR.select :air @15:27:00.04 PSV.dial PSR @15:27:01.40 Pulling 2ml of PSR.air @15:27:01.41 PSR.select :air @15:27:01.43 PS.reconfigure :default @15:27:01.48 PS.seek 2ml @15:27:22.76 delay 5 seconds @15:27:27.78 PTV.dial :puck @15:27:27.87 PSV.dial PTV @15:27:28.63 PBV.dial :waste1 @15:27:29.07 PS.seek 1.5ml @15:27:35.04 delay 3 seconds @15:27:38.06 PBV.dial between PSV and waste1 @15:27:38.43 delay 1 second @15:27:39.45 PSV.dial :waste1 @15:27:40.54 PS.seek 0ml @15:27:56.69 Processing Puck Heater at 28C before imaging @15:27:56.70 PH.coast @15:27:56.81 Imaging da puck in Processing Clamp while storing it to tube 4 @15:27:56.99 SC.reconfigure :tight @15:27:57.04 SC.seek 4 @15:28:00.64 SC.configure :limp @15:28:00.86 Elbow.reconfigure :tight @15:28:00.91 Elbow.seek 4 @15:28:01.09 CBV.dial :waste2b @15:28:01.91 CS.reconfigure :default @15:28:01.96 CS.seek 0ml @15:28:02.69 Forearm.reconfigure :tight @15:28:02.74 Forearm.seek 4 + 450 counts @15:28:03.17 delay 1:00 @15:28:03.80 SE.reconfigure :pick @15:28:03.86 SE.seek :beamBreak @15:28:06.58 SE.configure :default @15:28:06.74 Elbow.reconfigure :limp @15:28:06.79 Forearm.reconfigure :loose @15:28:06.84 Forearm.seek 4 @15:28:07.11 Elbow.hold @15:28:07.18 Elbow.reconfigure :tight @15:28:07.28 SE.seek 6.16 Pucks below Top Plate @15:28:07.76 Forearm.force -20 @15:28:07.80 Forearm.seek 4 + 305 counts @15:28:08.10 Forearm.reconfigure :tight @15:28:08.15 Forearm.seek 4 + 450 counts @15:28:08.36 SE.seek 7.16 Pucks below Top Plate @15:28:08.89 Forearm.reconfigure :loose @15:28:08.94 Forearm.seek :retract @15:28:09.89 Elbow.seek PC @15:28:12.43 Elbow.reconfigure :loose @15:28:12.53 Forearm.seek PC @15:28:13.49 Hand.close @15:28:14.06 PC.seek :open @15:28:18.84 Forearm.seek :retract @15:28:19.77 Elbow.reconfigure :tight @15:28:19.82 Elbow.seek 4 @15:28:22.42 Forearm.reconfigure :tight @15:28:22.47 Forearm.seek 4 + 450 counts @15:28:23.44 Hand.open @15:28:24.18 SE.seek 6.81 Pucks below Top Plate @15:28:24.47 SE.reconfigure :seal @15:28:24.53 SE.seek 5.85 Pucks below Top Plate @15:28:25.01 Lights, camera, ACTION! @15:28:25.04 PuckCamera: snap -bin=2,2 -auto /var/log/esp/da23dec0415h1000ml.tif @15:28:44.31 SX HX9/16 (USB:14): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15.967 seconds /var/log/esp/da23dec0415h1000ml.tif: TIFF Upload Complete @15:28:44.34 PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da23dec0415h1000ml15s.tif @15:28:44.58 Waiting >20 seconds for da to relinquish FlushPuck @15:28:59.99 SX HX9/16 (USB:14): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15 seconds /var/log/esp/midres/da23dec0415h1000ml15s.tif: TIFF Upload Complete @15:29:00.02 PuckCamera: snap -bin=2,2 20.0 /var/log/esp/midres/da23dec0415h1000ml20s.tif @15:29:03.20 CTV.dial :air,avoiding: :intake @15:29:04.59 Fix #2 of 2 (with RNAlater) for 2:30 @15:29:04.61 CSV.dial CSR @15:29:05.05 Pulling 2ml of CSR.RNAlater @15:29:05.06 CSR.select :RNAlater @15:29:05.18 CS.seek 2ml @15:29:11.20 delay 5 seconds @15:29:16.22 CSR.select :air @15:29:16.35 CS.reconfigure :fast @15:29:16.40 CS.seek 10ml @15:29:20.63 SX HX9/16 (USB:14): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 20 seconds /var/log/esp/midres/da23dec0415h1000ml20s.tif: TIFF Upload Complete @15:29:20.65 Gate.power :camera,:OFF @15:29:20.66 DAfans.email "15.967 sec auto-exposure",Subject: "da23dec0415h1000ml images!" @15:29:21.07 Elbow.seek 4 @15:29:21.41 Forearm.seek 4 + 450 counts @15:29:21.51 SE.reconfigure :gently @15:29:21.56 SE.seek 6.56 Pucks below Top Plate @15:29:22.31 Elbow.reconfigure :limp @15:29:22.37 Forearm.reconfigure :loose @15:29:22.42 Forearm.seek 4 @15:29:22.68 Elbow.hold @15:29:22.77 Elbow.reconfigure :tight @15:29:22.83 SE.reconfigure :default @15:29:22.88 SE.seek 6.75 Pucks below Top Plate @15:29:23.24 Forearm.force -20 @15:29:23.30 Forearm.seek 4 + 290 counts @15:29:23.61 Forearm.reconfigure :tight @15:29:23.66 Forearm.seek 4 + 450 counts @15:29:23.93 SE.seek :down @15:29:25.12 Forearm.reconfigure :loose @15:29:25.18 Forearm.seek :retract @15:29:26.75 SE.stop @15:29:26.98 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @15:29:27.41 Elbow.seek FlushPuck::Garage @15:29:29.39 Elbow.reconfigure :loose @15:29:29.61 Forearm.seek :garage @15:29:30.65 Hand.close @15:29:31.63 Forearm.seek :retract @15:29:32.65 Elbow.reconfigure :tight @15:29:32.75 Elbow.seek PC @15:29:34.16 Elbow.reconfigure :loose @15:29:34.37 Forearm.reconfigure :tight @15:29:34.47 Forearm.seek PC @15:29:36.06 PC.seek :closed @15:29:36.39 Pumping wcr puck from tube 5 up to 35psi... @15:29:36.46 CBV.dial SSV @15:29:37.81 CBV.dial between CSV and waste2b @15:29:39.05 CSV.dial between air and CTV @15:29:39.79 CS.seek 7.122ml @15:29:40.93 Processing Clamp onSpring/offSpring for 16/26 samples, on/off torque 1.65 @15:29:41.12 Hand.open @15:29:42.87 Forearm.reconfigure :loose @15:29:43.11 Forearm.seek :retract @15:29:44.12 PSR.select :air @15:29:44.17 PSV.dial PSR @15:29:44.70 Pulling 0.5ml of PSR.flush @15:29:44.72 PSR.select :flush @15:29:44.88 PS.seek 0.5ml @15:29:47.30 CTV.dial CSV,avoiding: :air @15:29:47.35 CSV.dial CTV @15:29:48.84 CS.reconfigure :pressurize @15:29:48.94 CS.seek 0ml @15:29:50.87 delay 5 seconds @15:29:55.90 PSR.select :air @15:29:56.09 PS.seek 2.5ml @15:29:57.11 upsync @15:30:06.91 delay 2:30 @15:30:17.44 delay 1 second @15:30:18.46 PRV.dial :waste2,avoiding: :ab2 @15:30:20.33 PTV.dial :PRVmixing @15:30:21.66 PSV.dial :mixloop @15:30:22.72 PS.seek :empty @15:30:49.16 PSR.select :air @15:30:49.18 PSV.dial PSR @15:30:50.28 Pulling 0.5ml of PSR.flush @15:30:50.29 PSR.select :flush @15:30:50.41 PS.seek 0.5ml @15:30:56.38 delay 5 seconds @15:31:01.40 PSR.select :air @15:31:01.52 PS.seek 2.5ml @15:31:22.72 delay 1 second @15:31:23.74 PBV.dial :waste1 @15:31:24.12 PTV.dial :mixing @15:31:24.88 PSV.dial :mixloop @15:31:25.95 PS.seek :empty @15:31:52.38 PSV.dial PSR @15:31:53.42 PSR.select :flush @15:31:53.55 PS.seek 1ml @15:32:04.66 PSR.select :air @15:32:04.77 PS.seek 2.5ml @15:32:20.97 PSV.dial PTV @15:32:21.74 PTV.dial :puck @15:32:22.49 PBV.dial :waste1 @15:32:22.57 PS.seek 0ml @15:32:36.93 CBV.dial :waste2b @15:32:37.27 CS.reconfigure :default @15:32:37.32 CS.seek 0ml @15:32:38.79 delay 1:00 @15:32:48.97 PSV.dial PSR @15:32:49.74 PSR.select :flush @15:32:49.86 PS.seek 0.5ml @15:32:55.88 PSR.select :air @15:32:56.54 PS.seek 2.5ml @15:33:17.72 PSV.dial PTV @15:33:18.50 PBV.dial PSV @15:33:19.07 PS.seek 0ml @15:33:38.82 CTV.dial :air,avoiding: :intake @15:33:40.20 Storing wcr puck in Collection Clamp to tube 4 @15:33:40.38 SC.reconfigure :tight @15:33:40.43 SC.seek 4 @15:33:40.64 SC.configure :limp @15:33:40.86 Elbow.reconfigure :tight @15:33:40.91 Elbow.seek 4 @15:33:43.52 Forearm.reconfigure :tight @15:33:43.57 Forearm.seek 4 + 450 counts @15:33:44.59 SE.reconfigure :pick @15:33:44.65 SE.seek :beamBreak @15:33:45.44 PSV.dial PBV @15:33:45.93 PS.seek 2.5ml @15:33:47.32 SE.configure :default @15:33:47.48 Elbow.reconfigure :limp @15:33:47.53 Forearm.reconfigure :loose @15:33:47.58 Forearm.seek 4 @15:33:47.88 Elbow.hold @15:33:47.94 Elbow.reconfigure :tight @15:33:48.05 SE.seek 7.16 Pucks below Top Plate @15:33:48.52 Forearm.force -20 @15:33:48.57 Forearm.seek 4 + 274 counts @15:33:48.85 Forearm.reconfigure :tight @15:33:48.91 Forearm.seek 4 + 450 counts @15:33:49.54 SE.seek 8.16 Pucks below Top Plate @15:33:50.06 Forearm.reconfigure :loose @15:33:50.11 Forearm.seek :retract @15:33:51.05 Elbow.seek CC @15:33:52.67 Elbow.reconfigure :loose @15:33:52.77 Forearm.seek CC @15:33:53.77 Hand.close @15:33:54.44 CC.seek :open @15:33:59.18 Forearm.seek :retract @15:34:00.18 Elbow.reconfigure :tight @15:34:00.23 Elbow.seek 4 @15:34:02.01 Elbow.reconfigure :limp @15:34:02.06 Forearm.seek 4 @15:34:03.06 Elbow.hold @15:34:03.13 Elbow.reconfigure :tight @15:34:03.18 Hand.open @15:34:03.92 Forearm.force -20 @15:34:03.97 Forearm.seek 4 + 304 counts @15:34:04.39 Forearm.reconfigure :tight @15:34:04.45 Forearm.seek 4 + 450 counts @15:34:04.73 SE.seek :down @15:34:05.86 Forearm.reconfigure :loose @15:34:05.91 Forearm.seek :retract @15:34:06.87 SE.stop @15:34:07.34
Starting finalPurge @15:34:12.34 PSV.dial :waste1 @15:34:13.09 PS.seek 0ml @15:34:26.96
Waiting >20 seconds for psFlush to relinquish FlushPuck @15:34:39.41 PSV.dial PSR @15:34:39.88 PSR.select :air @15:34:39.90 PS.seek 2.5ml @15:35:06.25 PSV.dial PTV @15:35:07.16 PBV.dial PSV @15:35:07.24 PS.seek 0ml @15:35:33.62 PSV.dial PBV @15:35:34.08 PS.seek 2.5ml @15:36:00.47 PSV.dial :waste1 @15:36:01.22 PS.seek 0ml @15:36:27.78 PSV.dial PSR @15:36:28.25 PSR.select :flush @15:36:28.37 PS.seek 1ml @15:36:39.47 PSR.select :air @15:36:39.59 PS.seek 2.5ml @15:36:55.72 PSV.dial PTV @15:36:56.45 PTV.dial :puck @15:36:56.53 PBV.dial :waste1 @15:36:57.10 PS.seek 0ml @15:37:23.43 PSV.dial PSR @15:37:24.20 PSR.select :flush @15:37:24.32 PS.seek 0.5ml @15:37:30.25 PSR.select :air @15:37:30.37 PS.seek 2.5ml @15:37:51.65 PSV.dial PTV @15:37:52.42 PBV.dial PSV @15:37:52.99 PS.seek 0ml @15:38:19.37 PSV.dial PBV @15:38:19.85 PS.seek 2.5ml @15:38:46.37 PSV.dial :waste1 @15:38:47.13 PS.seek 0ml @15:39:13.71 PSV.dial PSR @15:39:14.18 PSR.select :air @15:39:14.21 PS.seek 2.5ml @15:39:40.59 PSV.dial PTV @15:39:41.32 PBV.dial PSV @15:39:41.50 PS.seek 0ml @15:40:07.84 PSV.dial PBV @15:40:08.31 PS.seek 2.5ml @15:40:35.15 PSV.dial :waste1 @15:40:35.89 PS.seek 0ml @15:41:02.22
Loading FlushPuck into Collection Clamp from Processing Clamp @15:41:02.41 Elbow.seek PC @15:41:04.91 Elbow.reconfigure :loose @15:41:05.01 Forearm.seek PC @15:41:05.96 Hand.close @15:41:06.55 PC.seek :open @15:41:11.27 Forearm.seek :retract @15:41:12.19 Elbow.reconfigure :tight @15:41:12.23 Elbow.seek CC @15:41:15.96 Elbow.reconfigure :loose @15:41:16.06 Forearm.reconfigure :tight @15:41:16.10 Forearm.seek CC @15:41:17.40 CC.seek :closed @15:41:21.92 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.45 @15:41:21.98 Hand.open @15:41:22.60 Forearm.reconfigure :loose @15:41:22.65 Forearm.seek :retract @15:41:23.64 Flushing the Collection Valve::Manifold @15:41:23.77 CSV.dial CSR @15:41:24.20 Pulling 4.999ml of CSR.flush @15:41:24.21 CSR.select :flush @15:41:24.33 CS.seek 5ml @15:41:38.16 delay 5 seconds @15:41:43.17 CSR.select :air @15:41:43.34 CS.reconfigure :fast @15:41:43.39 CS.seek 7.5ml @15:41:50.15 CSV.dial :waste @15:41:51.90 CS.reconfigure :default @15:41:51.94 CS.seek 0ml @15:42:12.00 Flushing lower manifold's waste1 port @15:42:12.16 CSV.dial :flush @15:42:12.60 CS.reconfigure :fast @15:42:12.65 CS.seek 5ml @15:42:25.28 delay 5 seconds @15:42:30.30 CSV.dial :air @15:42:30.82 CS.seek 7.001ml @15:42:36.38 CSV.dial CTV @15:42:36.40
CTV.dial CSV @15:42:37.83 CBV.dial :waste1 @15:42:38.60 CS.seek 0ml @15:42:55.13 Flushing lower manifold's waste2 port @15:42:55.25 CSV.dial :flush @15:42:55.98 CS.seek 5ml @15:43:08.62 delay 5 seconds @15:43:13.64 CSV.dial :air @15:43:14.16 CS.seek 7.001ml @15:43:19.63 CSV.dial CTV @15:43:19.67
CTV.dial CSV @15:43:20.11 CBV.dial :waste2 @15:43:21.18 CS.seek 0ml @15:43:37.72 Flushing lower manifold's waste2b port @15:43:37.84 CSV.dial :flush @15:43:38.59 CS.seek 5ml @15:43:51.37 delay 5 seconds @15:43:56.39 CSV.dial :air @15:43:57.07 CS.seek 7ml @15:44:02.63 CSV.dial CTV @15:44:02.66
CTV.dial CSV @15:44:03.09 CBV.dial :waste2b @15:44:04.15 CS.seek 0ml @15:44:20.87 Disinfecting external Intake with kill @15:44:20.99 CSV.dial CSR @15:44:21.56 Pulling 8ml of CSR.kill @15:44:21.57 CSR.select :kill @15:44:21.69 CS.seek 8ml @15:44:41.50 delay 5 seconds @15:44:46.52 CSR.select :air @15:44:46.64 CS.seek 10ml @15:44:52.40 CSV.dial CBV @15:44:52.44
CBV.dial CSV @15:44:53.50 CTV.dial :intake @15:44:54.34 Intake.select :open @15:44:54.46 CS.seek 2ml @15:45:13.53 Intake.select :close @15:45:13.63 CTV.dial :air @15:45:14.41 CS.reconfigure :default @15:45:14.46 CS.seek 0ml @15:45:20.43 CSV.dial CSR @15:45:21.45 Pulling 5ml of CSR.flush @15:45:21.46 CSR.select :flush @15:45:21.58 CS.seek 5ml @15:45:35.40 delay 5 seconds @15:45:40.42 CSR.select :air @15:45:40.54 CS.seek 10ml @15:45:54.18 CSV.dial CBV @15:45:55.21 CS.seek 0ml @15:46:21.69 Garaging FlushPuck in Collection Clamp @15:46:21.76 Elbow.reconfigure :tight @15:46:21.80 Elbow.seek CC @15:46:22.10 Elbow.reconfigure :loose @15:46:22.19 Forearm.seek CC @15:46:23.15 Hand.close @15:46:23.79 CC.seek :open @15:46:28.42 Forearm.seek :retract @15:46:29.37 Elbow.reconfigure :tight @15:46:29.42 Elbow.seek :garage @15:46:32.28 Elbow.reconfigure :loose @15:46:32.38 Forearm.reconfigure :tight @15:46:32.43 Forearm.seek :garage @15:46:33.37 Hand.open @15:46:34.10 Forearm.reconfigure :loose @15:46:34.15 Forearm.seek :retract @15:46:35.10 Completed [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] begun 13:19:55 @15:46:35.16 Asleep after completing DA_WCR_120423 assays @15:46:35.18 GoodNews.email "{1 => 33,\n 2 => 33,\n 3 => 31,\n 4 => 9,\n 5 => 24,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@15:39:15, 31% flow, 15.7C, 31% humidity, 15.0psia, 13.749V, 0.327A, 146.531Ah, 4.49W\nSampled 575.0ml",Subject: "Asleep after completing DA_WCR_120423 assays" @15:46:39.57 upsync + ESPwhich DA_WCR_120423b.rb + fn=DA_WCR_120423b.rb + IFS=: + set /home/esp/esp2/mission . + fullPath=/home/esp/esp2/mission/DA_WCR_120423b.rb + [ -f /home/esp/esp2/mission/DA_WCR_120423b.rb ] + realpath /home/esp/esp2/mission/DA_WCR_120423b.rb + return 0 + scriptPath=/home/esp/esp2local/mission/DA_WCR_120423b.rb + exec ruby /home/esp/esp2/lib/esp.rb /home/esp/esp2local/mission/DA_WCR_120423b.rb @21:14:31.97UTC04-Dec-23
ESP2 v3.42 I2C Gateway v3.20 for core bus @21:14:34.56 Configuration for short puck jake 1/11/21 by brent@mbari.org /home/esp/esp2/type/shallow/jake/configure.rb @21:14:38.57 Polling Can every 10:00 @21:14:38.58 Cleanly exited at 21:14:09UTC04-Dec-23 @21:14:38.64 Loading /home/esp/esp2local/mission/DA_WCR_120423b.rb mission @21:14:38.69 GoodNews.email "Starting DA_WCR_120423b w/pucks in tube 5\n{}\nCTD data unavailable\nCan@21:14:38, 32% flow, 15.1C, 30% humidity, 15.0psia, 13.754V, 0.242A, 147.777Ah, 3.33W",Subject: "DA_WCR_120423b begun" @21:14:38.72
Preparing to sleep until + 00:00:05 @21:14:38.77 Asleep til + 00:00:05 @21:14:38.79 Continuing at 21:14 @21:14:39.07 Gate.power :core,:OFF @21:14:39.09 Gate.power :raw,:OFF Shutdown @21:14:42.09 GoodNews.email "Then, in tube 5: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_120423b.rb:14\n{1 => 33,\n 2 => 33,\n 3 => 31,\n 4 => 9,\n 5 => 24,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@21:14:38, 32% flow, 15.0C, 30% humidity, 15.0psia, 13.759V, 0.242A, 147.777Ah, 3.33W",Subject: "Asleep until 21:14" @21:14:43.42 upsync @21:14:43.68
Powering core bus @21:14:46.90 upsync @21:14:48.91
Gate.power :raw,:ON @21:14:49.63 CS.configure :default @21:14:49.68 PS.configure :default @21:14:49.73 SS.configure :default @21:14:49.78 CC.configure :default @21:14:49.83 PC.configure :default @21:14:49.90 Forearm.configure :loose @21:14:49.95 Elbow.configure :loose @21:14:50.03 SC.configure :loose @21:14:50.08 SE.configure :default @21:14:50.13 CH.configure :default @21:14:50.16 PH.configure :default @21:14:50.20 All dwarf controllers running firmware version 3.85 @21:14:50.32 Valve.reset @21:14:50.91 Intake.select :close @21:14:50.94 Exhaust.select :close @21:14:50.99 CTV.dial :air @21:14:51.10 PSV.dial :air @21:14:51.15 CSV.dial :waste1 @21:14:51.29
Forearm.home.seek :retract @21:14:51.66 SSV.dial :spit,avoiding: :exhaust @21:14:52.02 Hand.close @21:14:52.05
Elbow.reconfigure :loose @21:14:52.14 Elbow.jog 1500 @21:14:52.27 PS.home.seek 0ml @21:14:52.54 CS.reconfigure :fast @21:14:52.58 SS.home.seek 0ml @21:14:52.68 CS.home.seek 0ml @21:14:52.76
Elbow.reconfigure :loose @21:14:52.81 Elbow.home.seek :clear @21:14:56.14 SE.home.seek :down @21:14:56.76 SC.reconfigure :tight @21:14:56.81 SC.home.seek :initial @21:15:00.57 PS.stop @21:15:15.82
SC.configure :limp @21:15:15.88 Forearm.seek :retract @21:15:15.94 Elbow.seek :hammer @21:15:16.97 Forearm.reconfigure :tight @21:15:17.04 Forearm.seek retracted @21:15:17.49 PC.home.seek :open @21:15:17.50
CC.home.seek :open @21:15:20.99 Phase [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] @21:15:21.00 Notice.email "At 21:15:20 began in tube 5: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_120423b.rb:14\n{1 => 33,\n 2 => 33,\n 3 => 31,\n 4 => 9,\n 5 => 24,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@21:15:20, 32% flow, 15.1C, 30% humidity, 15.0psia, 13.725V, 0.272A, 147.781Ah, 3.74W",Subject: "Started DA_WCR_120423b phase initialPurge; da [1000, 1000] #da and wsr are run simultanelously" @21:15:21.03
Starting initialPurge @21:15:21.04 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @21:15:21.11 Hand.open @21:15:21.85 Elbow.reconfigure :tight @21:15:21.90 Elbow.seek FlushPuck::Garage @21:15:23.88 Elbow.reconfigure :loose @21:15:24.09 Forearm.reconfigure :loose @21:15:24.21 Forearm.seek :garage @21:15:25.20 Hand.close @21:15:25.43 upsync @21:15:26.00
Forearm.seek :retract @21:15:27.04 Elbow.reconfigure :tight @21:15:27.09 Elbow.seek CC @21:15:30.23 Elbow.reconfigure :loose @21:15:30.34 Forearm.reconfigure :tight @21:15:30.39 Forearm.seek CC @21:15:31.66 CC.seek :closed @21:15:36.19 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.45 @21:15:36.26 Hand.open @21:15:36.93 Forearm.reconfigure :loose @21:15:36.98 Forearm.seek :retract @21:15:37.97 Flushing the Collection Valve::Manifold @21:15:38.10 CSV.dial CSR @21:15:39.41 Pulling 5ml of CSR.flush CSR.select :flush @21:15:39.53 CS.reconfigure :default @21:15:39.58 CS.seek 5ml @21:15:53.81 delay 5 seconds @21:15:58.83 CSR.select :air @21:15:59.00 CS.reconfigure :fast @21:15:59.05 CS.seek 7.499ml @21:16:05.93 CSV.dial :waste @21:16:07.24 CS.reconfigure :default @21:16:07.29 CS.seek 0ml @21:16:27.34 Purging external Intake @21:16:27.47 CSV.dial :flush @21:16:27.91 CS.reconfigure :fast @21:16:27.96 CS.seek 10ml @21:16:52.47 delay 5 seconds @21:16:57.49 CSV.dial CBV @21:16:57.52
CBV.dial CSV @21:16:58.28 CTV.dial :intake @21:16:59.07 Intake.select :open @21:16:59.19 CS.seek 0ml @21:17:22.90 Intake.select :close @21:17:23.01 CTV.dial :air @21:17:23.81 Flushing upper manifold @21:17:23.93 CSV.dial :flush @21:17:24.67 CS.seek 5ml @21:17:37.40 delay 5 seconds @21:17:42.42 CSV.dial :air @21:17:43.06 CS.seek 7ml @21:17:48.59 CSV.dial CBV @21:17:48.61
CBV.dial CSV @21:17:49.65 CTV.dial :air @21:17:49.74 CS.seek 0ml @21:18:06.68 delay 7 seconds @21:18:13.71 CS.seek 3ml @21:18:21.68 CSV.dial :waste @21:18:22.14 CS.seek 0ml @21:18:30.14 CSV.dial :flush @21:18:30.60 CS.seek 3ml @21:18:38.59 delay 5 seconds @21:18:43.61 CSV.dial :air @21:18:44.10 CS.seek 5ml @21:18:49.87 CSV.dial :air @21:18:49.95 CS.seek 0ml @21:19:02.46 delay 7 seconds @21:19:09.49 CS.seek 3ml @21:19:17.49 CSV.dial :waste @21:19:18.23 CS.seek 0ml @21:19:25.93 Garaging FlushPuck in Collection Clamp @21:19:26.00 Elbow.reconfigure :tight @21:19:26.05 Elbow.seek CC @21:19:26.35 Elbow.reconfigure :loose @21:19:26.46 Forearm.seek CC @21:19:27.42 Hand.close @21:19:27.99 CC.seek :open @21:19:33.02 Forearm.seek :retract @21:19:34.00 Elbow.reconfigure :tight @21:19:34.05 Elbow.seek :garage @21:19:36.98 Elbow.reconfigure :loose @21:19:37.08 Forearm.reconfigure :tight @21:19:37.13 Forearm.seek :garage @21:19:38.15 Hand.open @21:19:38.79 Forearm.reconfigure :loose @21:19:38.84 Forearm.seek :retract @21:19:39.81 da sampling at most 1000ml, wcr at most 1000ml @21:19:39.85 Loading sh1 puck from tube 5 into Collection Clamp @21:19:39.87 DAfans.email "Started da in tube 5 at 21:19:39UTC04-Dec-23\n{1 => 33,\n 2 => 33,\n 3 => 31,\n 4 => 9,\n 5 => 24,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@21:15:20, 32% flow, 15.1C, 30% humidity, 15.0psia, 13.725V, 0.272A, 147.781Ah, 3.74W",Subject: "da sampling at most 1000ml, wcr at most 1000ml" @21:19:40.13
SC.reconfigure :tight @21:19:40.18 SC.seek 5 @21:19:43.84 SC.configure :limp @21:19:44.10 Elbow.reconfigure :tight @21:19:44.15 Elbow.seek 5 @21:19:44.38 upsync @21:19:46.19
Forearm.reconfigure :tight @21:19:46.24 Forearm.seek 5 + 450 counts @21:19:47.34 SE.reconfigure :pick @21:19:47.40 SE.seek :beamBreak @21:19:49.00 SE.configure :default @21:19:49.17 Elbow.seek 5 @21:19:49.37 Forearm.seek 5 + 300 counts @21:19:49.55 Forearm.reconfigure :loose @21:19:49.59 Hand.close @21:19:50.25 SE.seek :down @21:19:50.84
Forearm.seek :retract @21:19:51.51 SE.stop @21:19:51.83
Elbow.seek CC @21:19:53.40 Elbow.reconfigure :loose @21:19:53.50 Forearm.reconfigure :tight @21:19:53.55 Forearm.seek CC @21:19:54.87 CC.seek :closed @21:19:59.41 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.21 @21:19:59.42 Hand.open @21:20:00.04 Forearm.reconfigure :loose @21:20:00.09 Forearm.seek :retract @21:20:01.08 collectSample 1000ml w/bubble point of 24psi @21:20:01.09 Priming 7ml @21:20:01.10 Intake.select :close @21:20:01.11 Exhaust.select :close @21:20:01.12 SS.hold @21:20:01.33 Calibrating pressure and temperature sensors @21:20:01.36 CTV.dial :air @21:20:01.37 SSV.dial :spit,avoiding: :exhaust @21:20:01.56 SS.reconfigure :fast @21:20:01.61 SS.seek 5ml @21:20:04.37 SSV.dial CBV @21:20:04.39
CBV.dial SSV @21:20:05.74 delay 3.8 seconds @21:20:09.67 Sensor limits: Intake max 102.7psia; Outlet max 94.4psia @21:20:09.68 SSV.dial :spit @21:20:11.01 SS.seek 0ml @21:20:13.83 SSV.dial CBV @21:20:15.13 CTV.dial :intake @21:20:15.93 SSV.dial CBV @21:20:15.96
CBV.dial SSV @21:20:16.05 Allowing 40s for 7.0ml of remaining 7.0ml @21:20:16.06 Intake.select :open @21:20:18.23 Intake @14.2psia, vacuum from 8.7 to 11.7psi @21:20:18.24 SS.reconfigure :inhaling 2.20ml/s @21:20:18.29 SS.seek 7ml @21:20:24.86 Stroke completed -- waiting for pressure to equalize ... @21:20:24.87 SS.reconfigure :exhale @21:20:24.92 SS.hold @21:20:31.61 Intake.select :close @21:20:31.76 SSV.dial :exhaust @21:20:32.52 Exhaust.select :open @21:20:32.64 SS.seek 0ml @21:20:36.79 Exhaust.select :close @21:20:36.90 Sampling 1000.0ml @21:20:36.91 Intake.select :close @21:20:36.92 Exhaust.select :close @21:20:36.93 SS.reconfigure :default @21:20:36.98 SS.hold @21:20:37.19 CTV.dial :intake @21:20:37.42 SSV.dial CBV @21:20:37.44
CBV.dial SSV @21:20:38.24 Allowing 130s for 25.0ml of remaining 1000.0ml @21:20:38.25 Intake.select :open @21:20:40.42 Intake @14.7psia, vacuum from 9.2 to 12.2psi @21:20:40.43 SS.reconfigure :inhaling 2.20ml/s @21:20:40.48 SS.seek 25ml @21:20:47.43 Vacuum too high -- waiting up to 40 seconds @21:20:47.45 SS.reconfigure :exhale @21:20:47.50 SS.hold @21:20:52.75 Intake @16.4psia, vacuum from 10.0 to 13.0psi @21:20:52.76 SS.reconfigure :inhaling 1.76ml/s @21:20:52.81 SS.seek 25ml @21:20:56.68 Vacuum too high -- waiting up to 40 seconds @21:20:56.70 SS.reconfigure :exhale @21:20:56.75 SS.hold @21:21:00.24 Intake @17.0psia, vacuum from 10.0 to 13.0psi @21:21:00.26 SS.reconfigure :inhaling 1.41ml/s @21:21:00.31 SS.seek 25ml @21:21:04.15 Vacuum too high -- waiting up to 40 seconds @21:21:04.16 SS.reconfigure :exhale @21:21:04.22 SS.hold @21:21:06.38 Intake @16.7psia, vacuum from 10.0 to 13.0psi @21:21:06.40 SS.reconfigure :inhaling 1.13ml/s @21:21:06.45 SS.seek 25ml @21:21:11.55 Stroke completed -- waiting for pressure to equalize ... @21:21:11.56 SS.reconfigure :exhale @21:21:11.61 SS.hold @21:21:30.44 Intake.select :close @21:21:30.60 SSV.dial :exhaust @21:21:31.36 Exhaust.select :open @21:21:31.48 SS.seek 0ml @21:21:40.30 Exhaust.select :close @21:21:40.41 SSV.dial CBV @21:21:40.43
CBV.dial SSV @21:21:41.22 Allowing 130s for 25.0ml of remaining 975.0ml @21:21:41.23 Intake.select :open @21:21:43.40 Intake @15.5psia, vacuum from 10.0 to 13.0psi @21:21:43.42 SS.reconfigure :inhaling 1.13ml/s @21:21:43.47 SS.seek 25ml @21:22:08.64 Stroke completed -- waiting for pressure to equalize ... @21:22:08.65 SS.reconfigure :exhale @21:22:08.70 SS.hold @21:22:33.22 Intake.select :close @21:22:33.38 SSV.dial :exhaust @21:22:34.14 Exhaust.select :open @21:22:34.26 SS.seek 0ml @21:22:43.10 Exhaust.select :close @21:22:43.21 SSV.dial CBV @21:22:43.23
CBV.dial SSV @21:22:44.02 Allowing 130s for 25.0ml of remaining 950.0ml @21:22:44.03 Intake.select :open @21:22:46.19 Intake @15.6psia, vacuum from 10.0 to 13.0psi @21:22:46.21 SS.reconfigure :inhaling 1.13ml/s @21:22:46.25 SS.seek 25ml @21:23:11.14 Stroke completed -- waiting for pressure to equalize ... @21:23:11.15 SS.reconfigure :exhale @21:23:11.20 SS.hold @21:23:33.65 Intake.select :close @21:23:33.80 SSV.dial :exhaust @21:23:34.56 Exhaust.select :open @21:23:34.68 SS.seek 0ml @21:23:43.41 Exhaust.select :close @21:23:43.51 SSV.dial CBV @21:23:43.53
CBV.dial SSV @21:23:44.33 Allowing 130s for 25.0ml of remaining 925.0ml @21:23:44.34 Intake.select :open @21:23:46.51 Intake @15.6psia, vacuum from 10.0 to 13.0psi @21:23:46.52 SS.reconfigure :inhaling 1.13ml/s @21:23:46.57 SS.seek 25ml @21:24:11.45 Stroke completed -- waiting for pressure to equalize ... @21:24:11.46 SS.reconfigure :exhale @21:24:11.51 SS.hold @21:24:34.41 Intake.select :close @21:24:34.56 SSV.dial :exhaust @21:24:35.33 Exhaust.select :open @21:24:35.44 SS.seek 0ml @21:24:44.12 Exhaust.select :close @21:24:44.23 SSV.dial CBV @21:24:44.26
CBV.dial SSV @21:24:45.03 Allowing 130s for 25.0ml of remaining 900.0ml @21:24:45.04 Intake.select :open @21:24:47.21 Intake @15.6psia, vacuum from 10.0 to 13.0psi @21:24:47.23 SS.reconfigure :inhaling 1.13ml/s @21:24:47.27 SS.seek 25ml @21:25:11.97 Stroke completed -- waiting for pressure to equalize ... @21:25:11.98 SS.reconfigure :exhale @21:25:12.02 SS.hold @21:25:32.42 Intake.select :close @21:25:32.57 SSV.dial :exhaust @21:25:33.35 Exhaust.select :open @21:25:33.46 SS.seek 0ml @21:25:42.44 Exhaust.select :close @21:25:42.97 SSV.dial CBV @21:25:42.99
CBV.dial SSV @21:25:43.78 Allowing 130s for 25.0ml of remaining 875.0ml @21:25:43.79 Intake.select :open @21:25:45.96 Intake @15.6psia, vacuum from 10.0 to 13.0psi @21:25:45.98 SS.reconfigure :inhaling 1.13ml/s @21:25:46.03 SS.seek 25ml @21:26:10.43 Stroke completed -- waiting for pressure to equalize ... @21:26:10.44 SS.reconfigure :exhale @21:26:10.49 SS.hold @21:26:28.90 Intake.select :close @21:26:29.06 SSV.dial :exhaust @21:26:29.83 Exhaust.select :open @21:26:29.94 SS.seek 0ml @21:26:38.62 Exhaust.select :close @21:26:38.73 SSV.dial CBV @21:26:38.75
CBV.dial SSV @21:26:39.56 Allowing 130s for 25.0ml of remaining 850.0ml @21:26:39.57 Intake.select :open @21:26:41.74 Intake @15.7psia, vacuum from 10.0 to 13.0psi @21:26:41.76 SS.reconfigure :inhaling 1.13ml/s @21:26:41.81 SS.seek 25ml @21:27:06.49 Stroke completed -- waiting for pressure to equalize ... @21:27:06.50 SS.reconfigure :exhale @21:27:06.55 SS.hold @21:27:25.28 Intake.select :close @21:27:25.43 SSV.dial :exhaust @21:27:26.18 Exhaust.select :open @21:27:26.30 SS.seek 0ml @21:27:35.23 Exhaust.select :close @21:27:35.34 SSV.dial CBV @21:27:35.36
CBV.dial SSV @21:27:36.15 Allowing 130s for 25.0ml of remaining 825.0ml @21:27:36.16 Intake.select :open @21:27:38.33 Intake @15.7psia, vacuum from 10.0 to 13.0psi @21:27:38.35 SS.reconfigure :inhaling 1.13ml/s @21:27:38.39 SS.seek 25ml @21:27:59.30 Vacuum too high -- waiting up to 40 seconds @21:27:59.31 SS.reconfigure :exhale @21:27:59.37 SS.hold @21:28:04.76 Intake @14.0psia, vacuum from 8.5 to 11.5psi @21:28:04.77 SS.reconfigure :inhaling 0.91ml/s @21:28:04.82 SS.seek 25ml @21:28:09.26 Stroke completed -- waiting for pressure to equalize ... @21:28:09.27 SS.reconfigure :exhale @21:28:09.32 SS.hold @21:28:21.99 Intake.select :close @21:28:22.15 SSV.dial :exhaust @21:28:22.93 Exhaust.select :open @21:28:23.05 SS.seek 0ml @21:28:31.81 Exhaust.select :close @21:28:31.91 SSV.dial CBV @21:28:31.93
CBV.dial SSV @21:28:32.73 Allowing 130s for 25.0ml of remaining 800.0ml @21:28:32.74 Intake.select :open @21:28:34.91 Intake @15.7psia, vacuum from 10.0 to 13.0psi @21:28:34.93 SS.reconfigure :inhaling 0.91ml/s @21:28:35.40 SS.seek 25ml @21:29:05.16 Stroke completed -- waiting for pressure to equalize ... @21:29:05.17 SS.reconfigure :exhale @21:29:05.22 SS.hold @21:29:21.52 Intake.select :close @21:29:21.68 SSV.dial :exhaust @21:29:22.43 Exhaust.select :open @21:29:22.55 SS.seek 0ml @21:29:31.18 Exhaust.select :close @21:29:31.29 SSV.dial CBV @21:29:31.32
CBV.dial SSV @21:29:32.10 Allowing 130s for 25.0ml of remaining 775.0ml @21:29:32.12 Intake.select :open @21:29:34.28 Intake @15.6psia, vacuum from 10.0 to 13.0psi @21:29:34.30 SS.reconfigure :inhaling 0.91ml/s @21:29:34.35 SS.seek 25ml @21:30:04.08 Stroke completed -- waiting for pressure to equalize ... @21:30:04.10 SS.reconfigure :exhale @21:30:04.14 SS.hold @21:30:20.54 Intake.select :close @21:30:20.70 SSV.dial :exhaust @21:30:21.46 Exhaust.select :open @21:30:21.58 SS.seek 0ml @21:30:30.20 Exhaust.select :close @21:30:30.31 SSV.dial CBV @21:30:30.33
CBV.dial SSV @21:30:31.13 Allowing 130s for 25.0ml of remaining 750.0ml @21:30:31.14 Intake.select :open @21:30:33.31 Intake @15.6psia, vacuum from 10.0 to 13.0psi @21:30:33.33 SS.reconfigure :inhaling 0.91ml/s @21:30:33.38 SS.seek 25ml @21:31:03.19 Stroke completed -- waiting for pressure to equalize ... @21:31:03.20 SS.reconfigure :exhale @21:31:03.25 SS.hold @21:31:22.02 Intake.select :close @21:31:22.17 SSV.dial :exhaust @21:31:22.93 Exhaust.select :open @21:31:23.05 SS.seek 0ml @21:31:32.01 Exhaust.select :close @21:31:32.11 SSV.dial CBV @21:31:32.13
CBV.dial SSV @21:31:32.93 Allowing 130s for 25.0ml of remaining 725.0ml @21:31:32.94 Intake.select :open @21:31:35.11 Intake @15.6psia, vacuum from 10.0 to 13.0psi @21:31:35.13 SS.reconfigure :inhaling 0.91ml/s @21:31:35.17 SS.seek 25ml @21:32:04.47 Stroke completed -- waiting for pressure to equalize ... @21:32:04.48 SS.reconfigure :exhale @21:32:04.53 SS.hold @21:32:22.95 Intake.select :close @21:32:23.21 SSV.dial :exhaust @21:32:23.98 Exhaust.select :open @21:32:24.09 SS.seek 0ml @21:32:32.83 Exhaust.select :close @21:32:32.94 SSV.dial CBV @21:32:32.97
CBV.dial SSV @21:32:33.76 Allowing 130s for 25.0ml of remaining 700.0ml @21:32:33.77 Intake.select :open @21:32:35.93 Intake @15.6psia, vacuum from 10.0 to 13.0psi @21:32:35.95 SS.reconfigure :inhaling 0.91ml/s @21:32:35.99 SS.seek 25ml @21:33:05.75 Stroke completed -- waiting for pressure to equalize ... @21:33:05.76 SS.reconfigure :exhale @21:33:05.81 SS.hold @21:33:24.20 Intake.select :close @21:33:24.35 SSV.dial :exhaust @21:33:25.13 Exhaust.select :open @21:33:25.25 SS.seek 0ml @21:33:34.00 Exhaust.select :close @21:33:34.12 SSV.dial CBV @21:33:34.14
CBV.dial SSV @21:33:34.94 Allowing 130s for 25.0ml of remaining 675.0ml @21:33:34.95 Intake.select :open @21:33:37.12 Intake @15.6psia, vacuum from 10.0 to 13.0psi @21:33:37.14 SS.reconfigure :inhaling 0.91ml/s @21:33:37.19 SS.seek 25ml @21:34:06.89 Stroke completed -- waiting for pressure to equalize ... @21:34:06.90 SS.reconfigure :exhale @21:34:06.95 SS.hold @21:34:25.33 Intake.select :close @21:34:25.49 SSV.dial :exhaust @21:34:26.27 Exhaust.select :open @21:34:26.81 SS.seek 0ml @21:34:35.47 Exhaust.select :close @21:34:35.58 SSV.dial CBV @21:34:35.62
CBV.dial SSV @21:34:36.38 Allowing 130s for 25.0ml of remaining 650.0ml @21:34:36.39 Intake.select :open @21:34:38.56 Intake @15.6psia, vacuum from 10.0 to 13.0psi @21:34:38.58 SS.reconfigure :inhaling 0.91ml/s @21:34:38.62 SS.seek 25ml @21:35:08.70 Stroke completed -- waiting for pressure to equalize ... @21:35:08.71 SS.reconfigure :exhale @21:35:08.76 SS.hold @21:35:29.19 Intake.select :close @21:35:29.35 SSV.dial :exhaust @21:35:30.13 Exhaust.select :open @21:35:30.24 SS.seek 0ml @21:35:39.27 Exhaust.select :close @21:35:39.37 SSV.dial CBV @21:35:39.40
CBV.dial SSV @21:35:40.17 Allowing 130s for 25.0ml of remaining 625.0ml @21:35:40.19 Intake.select :open @21:35:42.35 Intake @15.6psia, vacuum from 10.0 to 13.0psi @21:35:42.72 SS.reconfigure :inhaling 0.91ml/s @21:35:42.84 SS.seek 25ml @21:36:12.05 Stroke completed -- waiting for pressure to equalize ... @21:36:12.06 SS.reconfigure :exhale @21:36:12.11 SS.hold @21:36:34.56 Intake.select :close @21:36:34.72 SSV.dial :exhaust @21:36:35.49 Exhaust.select :open @21:36:35.60 SS.seek 0ml @21:36:44.42 Exhaust.select :close @21:36:44.54 SSV.dial CBV @21:36:44.56
CBV.dial SSV @21:36:45.35 Allowing 130s for 25.0ml of remaining 600.0ml @21:36:45.36 Intake.select :open @21:36:47.52 Intake @15.6psia, vacuum from 10.0 to 13.0psi @21:36:47.53 SS.reconfigure :inhaling 0.91ml/s @21:36:47.58 SS.seek 25ml @21:37:17.09 Stroke completed -- waiting for pressure to equalize ... @21:37:17.10 SS.reconfigure :exhale @21:37:17.15 SS.hold @21:37:39.86 Intake.select :close @21:37:40.02 SSV.dial :exhaust @21:37:40.78 Exhaust.select :open @21:37:40.90 SS.seek 0ml @21:37:49.89 Exhaust.select :close @21:37:50.00 SSV.dial CBV @21:37:50.02
CBV.dial SSV @21:37:50.81 Allowing 130s for 25.0ml of remaining 575.0ml @21:37:50.82 Intake.select :open @21:37:52.99 Intake @15.6psia, vacuum from 10.0 to 13.0psi @21:37:53.01 SS.reconfigure :inhaling 0.91ml/s @21:37:53.05 SS.seek 25ml @21:38:17.29 Vacuum too high -- waiting up to 40 seconds @21:38:17.30 SS.reconfigure :exhale @21:38:17.35 SS.hold @21:38:24.19 Intake @14.7psia, vacuum from 9.2 to 12.2psi @21:38:24.21 SS.reconfigure :inhaling 0.73ml/s @21:38:24.26 SS.seek 25ml @21:38:31.20 Stroke completed -- waiting for pressure to equalize ... @21:38:31.21 SS.reconfigure :exhale @21:38:31.26 SS.hold @21:38:47.94 Intake.select :close @21:38:48.17 SSV.dial :exhaust @21:38:48.95 Exhaust.select :open @21:38:49.07 SS.seek 0ml @21:38:57.84 Exhaust.select :close @21:38:57.95 SSV.dial CBV @21:38:57.99
CBV.dial SSV @21:38:58.76 Allowing 130s for 25.0ml of remaining 550.0ml @21:38:58.78 Intake.select :open @21:39:00.94 Intake @15.5psia, vacuum from 10.0 to 13.0psi @21:39:00.95 SS.reconfigure :inhaling 0.73ml/s @21:39:01.00 SS.seek 25ml @21:39:37.43 Stroke completed -- waiting for pressure to equalize ... @21:39:37.44 SS.reconfigure :exhale @21:39:37.49 SS.hold @21:39:59.94 Intake.select :close @21:40:00.10 SSV.dial :exhaust @21:40:01.30 Exhaust.select :open @21:40:01.41 SS.seek 0ml @21:40:10.29 Exhaust.select :close @21:40:10.41 SSV.dial CBV @21:40:10.43
CBV.dial SSV @21:40:11.22 Allowing 130s for 25.0ml of remaining 525.0ml @21:40:11.23 Intake.select :open @21:40:13.39 Intake @15.6psia, vacuum from 10.0 to 13.0psi @21:40:13.41 SS.reconfigure :inhaling 0.73ml/s @21:40:13.45 SS.seek 25ml @21:40:45.35 Vacuum too high -- waiting up to 40 seconds @21:40:45.36 SS.reconfigure :exhale @21:40:45.42 SS.hold @21:40:52.02 Intake @15.1psia, vacuum from 9.6 to 12.6psi @21:40:52.04 SS.reconfigure :inhaling 0.59ml/s @21:40:52.09 SS.seek 25ml @21:40:57.51 Stroke completed -- waiting for pressure to equalize ... @21:40:57.52 SS.reconfigure :exhale @21:40:57.57 SS.hold @21:41:12.37 Intake.select :close @21:41:12.52 SSV.dial :exhaust @21:41:13.29 Exhaust.select :open @21:41:13.41 SS.seek 0ml @21:41:22.23 Exhaust.select :close @21:41:22.34 SSV.dial CBV @21:41:22.36
CBV.dial SSV @21:41:23.17 Allowing 130s for 25.0ml of remaining 500.0ml @21:41:23.18 Intake.select :open @21:41:25.35 Intake @15.7psia, vacuum from 10.0 to 13.0psi @21:41:25.37 SS.reconfigure :inhaling 0.59ml/s @21:41:25.42 SS.seek 25ml @21:42:10.31 Stroke completed -- waiting for pressure to equalize ... @21:42:10.32 SS.reconfigure :exhale @21:42:10.37 SS.hold @21:42:28.73 Intake.select :close @21:42:28.89 SSV.dial :exhaust @21:42:29.67 Exhaust.select :open @21:42:29.78 SS.seek 0ml @21:42:38.57 Exhaust.select :close @21:42:38.68 SSV.dial CBV @21:42:38.71
CBV.dial SSV @21:42:39.48 Allowing 130s for 25.0ml of remaining 475.0ml @21:42:39.49 Intake.select :open @21:42:41.66 Intake @15.6psia, vacuum from 10.0 to 13.0psi @21:42:41.68 SS.reconfigure :inhaling 0.59ml/s @21:42:41.72 SS.seek 25ml @21:43:26.68 Stroke completed -- waiting for pressure to equalize ... @21:43:26.69 SS.reconfigure :exhale @21:43:26.74 SS.hold @21:43:47.19 Intake.select :close @21:43:47.35 SSV.dial :exhaust @21:43:48.11 Exhaust.select :open @21:43:48.23 SS.seek 0ml @21:43:56.98 Exhaust.select :close @21:43:57.08 SSV.dial CBV @21:43:57.10
CBV.dial SSV @21:43:57.88 Allowing 130s for 25.0ml of remaining 450.0ml @21:43:57.90 Intake.select :open @21:44:00.06 Intake @15.7psia, vacuum from 10.0 to 13.0psi @21:44:00.08 SS.reconfigure :inhaling 0.59ml/s @21:44:00.13 SS.seek 25ml @21:44:44.85 Stroke completed -- waiting for pressure to equalize ... @21:44:44.86 SS.reconfigure :exhale @21:44:44.91 SS.hold @21:45:05.74 Intake.select :close @21:45:05.89 SSV.dial :exhaust @21:45:06.65 Exhaust.select :open @21:45:06.77 SS.seek 0ml @21:45:15.52 Exhaust.select :close @21:45:15.63 SSV.dial CBV @21:45:15.66
CBV.dial SSV @21:45:16.43 Allowing 130s for 25.0ml of remaining 425.0ml @21:45:16.44 Intake.select :open @21:45:18.61 Intake @15.7psia, vacuum from 10.0 to 13.0psi @21:45:18.62 SS.reconfigure :inhaling 0.59ml/s @21:45:18.67 SS.seek 25ml @21:45:57.06 Vacuum too high -- waiting up to 40 seconds @21:45:57.08 SS.reconfigure :exhale @21:45:57.13 SS.hold @21:46:05.69 SS.reconfigure :inhaling 0.47ml/s @21:46:05.74 SS.seek 25ml @21:46:14.14 Stroke completed -- waiting for pressure to equalize ... @21:46:14.16 SS.reconfigure :exhale @21:46:14.20 SS.hold @21:46:30.66 Intake.select :close @21:46:30.82 SSV.dial :exhaust @21:46:31.60 Exhaust.select :open @21:46:31.72 SS.seek 0ml @21:46:40.43 Exhaust.select :close @21:46:40.53 SSV.dial CBV @21:46:40.56
CBV.dial SSV @21:46:41.35 Allowing 130s for 25.0ml of remaining 400.0ml @21:46:41.36 Intake.select :open @21:46:43.53 Intake @15.7psia, vacuum from 10.0 to 13.0psi @21:46:43.55 SS.reconfigure :inhaling 0.47ml/s @21:46:43.60 SS.seek 25ml @21:47:39.38 Stroke completed -- waiting for pressure to equalize ... @21:47:39.39 SS.reconfigure :exhale @21:47:39.44 SS.hold @21:47:59.83 Intake.select :close @21:47:59.98 SSV.dial :exhaust @21:48:00.75 Exhaust.select :open @21:48:00.86 SS.seek 0ml @21:48:09.88 Exhaust.select :close @21:48:09.99 SSV.dial CBV @21:48:10.01
CBV.dial SSV @21:48:10.82 Allowing 130s for 25.0ml of remaining 375.0ml @21:48:10.83 Intake.select :open @21:48:12.99 Intake @15.7psia, vacuum from 10.0 to 13.0psi @21:48:13.01 SS.reconfigure :inhaling 0.47ml/s @21:48:13.06 SS.seek 25ml @21:49:08.19 Stroke completed -- waiting for pressure to equalize ... @21:49:08.20 SS.reconfigure :exhale @21:49:08.25 SS.hold @21:49:28.71 Intake.select :close @21:49:28.87 SSV.dial :exhaust @21:49:29.64 Exhaust.select :open @21:49:29.75 SS.seek 0ml @21:49:38.49 Exhaust.select :close @21:49:38.59 SSV.dial CBV @21:49:38.61
CBV.dial SSV @21:49:39.39 Allowing 130s for 25.0ml of remaining 350.0ml @21:49:39.40 Intake.select :open @21:49:41.57 Intake @15.6psia, vacuum from 10.0 to 13.0psi @21:49:41.59 SS.reconfigure :inhaling 0.47ml/s @21:49:41.63 SS.seek 25ml @21:50:37.17 Stroke completed -- waiting for pressure to equalize ... @21:50:37.18 SS.reconfigure :exhale @21:50:37.23 SS.hold @21:50:59.74 Intake.select :close @21:50:59.89 SSV.dial :exhaust @21:51:00.67 Exhaust.select :open @21:51:00.79 SS.seek 0ml @21:51:09.83 Exhaust.select :close @21:51:09.94 SSV.dial CBV @21:51:09.96
CBV.dial SSV @21:51:10.75 Allowing 130s for 25.0ml of remaining 325.0ml @21:51:10.76 Intake.select :open @21:51:12.93 Intake @15.8psia, vacuum from 10.0 to 13.0psi @21:51:12.95 SS.reconfigure :inhaling 0.47ml/s @21:51:12.99 SS.seek 25ml @21:52:07.87 Stroke completed -- waiting for pressure to equalize ... @21:52:07.88 SS.reconfigure :exhale @21:52:07.93 SS.hold @21:52:34.49 Intake.select :close @21:52:34.65 SSV.dial :exhaust @21:52:35.41 Exhaust.select :open @21:52:35.53 SS.seek 0ml @21:52:44.30 Exhaust.select :close @21:52:44.41 SSV.dial CBV @21:52:44.44
CBV.dial SSV @21:52:45.19 Allowing 130s for 25.0ml of remaining 300.0ml @21:52:45.20 Intake.select :open @21:52:47.36 Intake @15.7psia, vacuum from 10.0 to 13.0psi @21:52:47.38 SS.reconfigure :inhaling 0.47ml/s @21:52:47.42 SS.seek 25ml @21:53:42.95 Stroke completed -- waiting for pressure to equalize ... @21:53:42.96 SS.reconfigure :exhale @21:53:43.01 SS.hold @21:54:09.97 Intake.select :close @21:54:10.13 SSV.dial :exhaust @21:54:10.89 Exhaust.select :open @21:54:11.00 SS.seek 0ml @21:54:20.07 Exhaust.select :close @21:54:20.19 SSV.dial CBV @21:54:20.23
CBV.dial SSV @21:54:20.97 Allowing 130s for 25.0ml of remaining 275.0ml @21:54:20.98 Intake.select :open @21:54:23.14 Intake @15.8psia, vacuum from 10.0 to 13.0psi @21:54:23.16 SS.reconfigure :inhaling 0.47ml/s @21:54:23.20 SS.seek 25ml @21:55:18.20 Stroke completed -- waiting for pressure to equalize ... @21:55:18.21 SS.reconfigure :exhale @21:55:18.26 SS.hold @21:55:46.84 Intake.select :close @21:55:46.99 SSV.dial :exhaust @21:55:47.76 Exhaust.select :open @21:55:47.88 SS.seek 0ml @21:55:56.67 Exhaust.select :close @21:55:56.77 SSV.dial CBV @21:55:56.80
CBV.dial SSV @21:55:57.57 Allowing 130s for 25.0ml of remaining 250.0ml @21:55:57.58 Intake.select :open @21:55:59.75 Intake @15.8psia, vacuum from 10.0 to 13.0psi @21:55:59.77 SS.reconfigure :inhaling 0.47ml/s @21:55:59.82 SS.seek 25ml @21:56:55.91 Stroke completed -- waiting for pressure to equalize ... @21:56:55.92 SS.reconfigure :exhale @21:56:55.97 SS.hold @21:57:26.69 Intake.select :close @21:57:26.85 SSV.dial :exhaust @21:57:27.62 Exhaust.select :open @21:57:27.74 SS.seek 0ml @21:57:36.45 Exhaust.select :close @21:57:36.55 SSV.dial CBV @21:57:36.57
CBV.dial SSV @21:57:37.43 Allowing 130s for 25.0ml of remaining 225.0ml @21:57:37.44 Intake.select :open @21:57:39.61 Intake @15.8psia, vacuum from 10.0 to 13.0psi @21:57:39.63 SS.reconfigure :inhaling 0.47ml/s @21:57:39.68 SS.seek 25ml @21:58:30.58 Vacuum too high -- waiting up to 40 seconds @21:58:30.59 SS.reconfigure :exhale @21:58:30.64 SS.hold @21:58:45.23 Intake @16.9psia, vacuum from 10.0 to 13.0psi @21:58:45.24 SS.reconfigure :inhaling 0.38ml/s @21:58:45.29 SS.seek 25ml @21:58:51.26 Stroke completed -- waiting for pressure to equalize ... @21:58:51.27 SS.reconfigure :exhale @21:58:51.31 SS.hold @21:59:14.11 Intake.select :close @21:59:14.26 SSV.dial :exhaust @21:59:15.02 Exhaust.select :open @21:59:15.14 SS.seek 0ml @21:59:23.94 Exhaust.select :close @21:59:24.05 SSV.dial CBV @21:59:24.10
CBV.dial SSV @21:59:24.83 Allowing 130s for 25.0ml of remaining 200.0ml @21:59:24.85 Intake.select :open @21:59:27.01 Intake @15.8psia, vacuum from 10.0 to 13.0psi @21:59:27.02 SS.reconfigure :inhaling 0.38ml/s @21:59:27.07 SS.seek 25ml @22:00:36.21 Stroke completed -- waiting for pressure to equalize ... @22:00:36.22 SS.reconfigure :exhale @22:00:36.27 SS.hold @22:01:04.85 Intake.select :close @22:01:05.01 SSV.dial :exhaust @22:01:05.79 Exhaust.select :open @22:01:05.90 SS.seek 0ml @22:01:14.74 Exhaust.select :close @22:01:14.85 SSV.dial CBV @22:01:14.87
CBV.dial SSV @22:01:15.69 Allowing 130s for 25.0ml of remaining 175.0ml @22:01:15.70 Intake.select :open @22:01:17.87 Intake @15.8psia, vacuum from 10.0 to 13.0psi @22:01:17.89 SS.reconfigure :inhaling 0.38ml/s @22:01:17.94 SS.seek 25ml @22:02:26.99 Stroke completed -- waiting for pressure to equalize ... @22:02:27.00 SS.reconfigure :exhale @22:02:27.04 SS.hold @22:02:57.66 Intake.select :close @22:02:57.82 SSV.dial :exhaust @22:02:58.60 Exhaust.select :open @22:02:58.71 SS.seek 0ml @22:03:07.58 Exhaust.select :close @22:03:07.69 SSV.dial CBV @22:03:07.72
CBV.dial SSV @22:03:08.49 Allowing 130s for 25.0ml of remaining 150.0ml @22:03:08.50 Intake.select :open @22:03:10.67 Intake @15.8psia, vacuum from 10.0 to 13.0psi @22:03:10.68 SS.reconfigure :inhaling 0.38ml/s @22:03:10.73 SS.seek 25ml @22:04:19.91 Stroke completed -- waiting for pressure to equalize ... @22:04:19.92 SS.reconfigure :exhale @22:04:19.96 SS.hold @22:04:55.17 Intake.select :close @22:04:55.33 SSV.dial :exhaust @22:04:56.09 Exhaust.select :open @22:04:56.21 SS.seek 0ml @22:05:05.11 Exhaust.select :close @22:05:05.22 SSV.dial CBV @22:05:05.24
CBV.dial SSV @22:05:06.03 Allowing 130s for 25.0ml of remaining 125.0ml @22:05:06.04 Intake.select :open @22:05:08.21 Intake @16.0psia, vacuum from 10.0 to 13.0psi @22:05:08.23 SS.reconfigure :inhaling 0.38ml/s @22:05:08.27 SS.seek 25ml @22:06:17.20 Stroke completed -- waiting for pressure to equalize ... @22:06:17.21 SS.reconfigure :exhale @22:06:17.26 SS.hold @22:06:54.03 Intake.select :close @22:06:54.19 SSV.dial :exhaust @22:06:54.95 Exhaust.select :open @22:06:55.07 SS.seek 0ml @22:07:04.11 Exhaust.select :close @22:07:04.22 SSV.dial CBV @22:07:04.24
CBV.dial SSV @22:07:05.03 Allowing 130s for 25.0ml of remaining 100.0ml @22:07:05.04 Intake.select :open @22:07:07.21 Intake @15.8psia, vacuum from 10.0 to 13.0psi @22:07:07.23 SS.reconfigure :inhaling 0.38ml/s @22:07:07.27 SS.seek 25ml @22:08:16.09 Stroke completed -- waiting for pressure to equalize ... @22:08:16.10 SS.reconfigure :exhale @22:08:16.15 SS.hold @22:08:55.08 Intake.select :close @22:08:55.23 SSV.dial :exhaust @22:08:56.05 Exhaust.select :open @22:08:56.16 SS.seek 0ml @22:09:05.01 Exhaust.select :close @22:09:05.12 SSV.dial CBV @22:09:05.14
CBV.dial SSV @22:09:05.93 Allowing 130s for 25.0ml of remaining 75.0ml @22:09:05.94 Intake.select :open @22:09:08.11 Intake @15.9psia, vacuum from 10.0 to 13.0psi @22:09:08.13 SS.reconfigure :inhaling 0.38ml/s @22:09:08.18 SS.seek 25ml @22:10:14.60 Vacuum too high -- waiting up to 40 seconds @22:10:14.61 SS.reconfigure :exhale @22:10:14.66 SS.hold @22:10:35.62 Intake @17.3psia, vacuum from 10.0 to 13.0psi @22:10:35.64 SS.reconfigure :inhaling 0.31ml/s @22:10:35.69 SS.seek 25ml @22:10:39.27 Stroke completed -- waiting for pressure to equalize ... @22:10:39.28 SS.reconfigure :exhale @22:10:39.33 SS.hold @22:11:01.78 Intake.select :close @22:11:01.93 SSV.dial :exhaust @22:11:02.70 Exhaust.select :open @22:11:02.81 SS.seek 0ml @22:11:11.63 Exhaust.select :close @22:11:11.74 SSV.dial CBV @22:11:11.77
CBV.dial SSV @22:11:12.54 Allowing 130s for 25.0ml of remaining 50.0ml @22:11:12.55 Intake.select :open @22:11:14.72 Intake @15.9psia, vacuum from 10.0 to 13.0psi @22:11:14.74 SS.reconfigure :inhaling 0.31ml/s @22:11:14.78 SS.seek 25ml @22:12:42.43 Stroke completed -- waiting for pressure to equalize ... @22:12:42.44 SS.reconfigure :exhale @22:12:42.49 SS.hold @22:13:19.27 Intake.select :close @22:13:19.43 SSV.dial :exhaust @22:13:20.19 Exhaust.select :open @22:13:20.31 SS.seek 0ml @22:13:29.13 Exhaust.select :close @22:13:29.24 SSV.dial CBV @22:13:29.27
CBV.dial SSV @22:13:30.04 Allowing 130s for 25.0ml of remaining 25.0ml @22:13:30.05 Intake.select :open @22:13:32.22 Intake @16.0psia, vacuum from 10.0 to 13.0psi @22:13:32.23 SS.reconfigure :inhaling 0.31ml/s @22:13:32.28 SS.seek 24.97ml @22:15:00.11 Stroke completed -- waiting for pressure to equalize ... @22:15:00.12 SS.reconfigure :exhale @22:15:00.17 SS.hold @22:15:39.44 Intake.select :close @22:15:39.59 SSV.dial :exhaust @22:15:40.37 Exhaust.select :open @22:15:40.49 SS.seek 0ml @22:15:49.33 Exhaust.select :close @22:15:49.44 Sampled 1000.0ml @22:15:49.46 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) Intake.select :close @22:15:49.53 CSV.dial :waste @22:15:49.67 SSV.dial CBV,avoiding: :exhaust @22:15:49.69
CBV.dial SSV @22:15:50.26 CS.seek 0ml @22:15:50.33 CSV.dial :air @22:15:51.20 CS.seek 10ml @22:15:51.99
CTV.dial :air @22:15:52.74 delay 15 seconds @22:16:07.76 SSV.dial :spit,avoiding: :exhaust @22:16:09.11 CTV.dial CSV,avoiding: :intake @22:16:09.14
SS.seek 0ml @22:16:09.27 SSV.dial CBV,avoiding: :exhaust @22:16:15.91 SSV.dial between CBV and plug @22:16:16.40 CBV.dial between SSV and waste2 @22:16:16.80 CSV.dial between air and CTV @22:16:17.14 CS.seek 8.386ml @22:16:21.65 CTV.dial CSV,avoiding: :air @22:16:21.67
CSV.dial CTV @22:16:22.06 CS.reconfigure :pressurize @22:16:22.11 CS.seek 0ml @22:16:36.05 BubblePoint: Intake @24.2psia, Outlet @0.6psia @22:16:36.06 CBV.dial SSV @22:16:36.40 SS.reconfigure :default @22:16:36.45 SS.seek 10ml @22:16:42.08 delay 15 seconds @22:16:57.21 Evacuated: Intake @23.7psia, Outlet @23.1psia @22:16:57.22 SSV.dial :spit,avoiding: :exhaust @22:16:58.26 SS.reconfigure :exhale @22:16:58.31 SS.seek 0ml @22:17:03.35 SS.reconfigure :fast @22:17:03.40 SS.seek 5ml @22:17:06.34 SSV.dial CBV,avoiding: :exhaust @22:17:06.36
CBV.dial SSV @22:17:07.71 CTV.dial :air,avoiding: :intake @22:17:09.02 CSV.dial CTV @22:17:09.08
SS.seek 10ml @22:17:10.23 CSV.dial :air @22:17:10.67 CS.reconfigure :fast @22:17:10.71 CS.seek 0ml @22:17:11.66
SSV.dial :spit,avoiding: :exhaust @22:17:12.99 SS.reconfigure :exhale @22:17:13.04 SS.seek 0ml @22:17:18.04 SS.reconfigure :fast @22:17:18.09 SS.seek 5ml @22:17:18.72 CS.stop @22:17:20.92 SSV.dial CBV,avoiding: :exhaust @22:17:20.94
CBV.dial SSV @22:17:23.71 SSV.dial :spit,avoiding: :exhaust @22:17:25.04 SS.reconfigure :default @22:17:25.09 SS.seek 0ml @22:17:28.34 SS.stop @22:17:28.39 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @22:17:28.40 Intake.select :close @22:17:28.46 CSV.dial :waste @22:17:28.59 SSV.dial CBV,avoiding: :exhaust @22:17:28.61
CBV.dial SSV @22:17:29.23 CS.seek 0ml @22:17:29.29 CSV.dial :air @22:17:29.96
CTV.dial :air @22:17:30.05 delay 15 seconds @22:17:30.07 CS.seek 10ml @22:17:45.07
SSV.dial :spit,avoiding: :exhaust @22:17:46.39 CTV.dial CSV,avoiding: :intake @22:17:46.42
SS.reconfigure :exhale @22:17:46.51 SS.seek 0ml @22:17:46.91 SSV.dial CBV,avoiding: :exhaust @22:17:54.57 SSV.dial between CBV and plug @22:17:55.07 CBV.dial between SSV and waste2 @22:17:55.47 CSV.dial between air and CTV @22:17:55.79 CS.seek 7.456ml @22:18:02.42 CTV.dial CSV,avoiding: :air @22:18:02.44
CSV.dial CTV @22:18:02.82 CS.reconfigure :pressurize @22:18:02.87 CS.seek 0ml @22:18:15.95 BubblePoint: Intake @24.3psia, Outlet @3.1psia @22:18:15.96 CBV.dial SSV @22:18:16.31 SS.reconfigure :default @22:18:16.35 SS.seek 10ml @22:18:21.95 delay 15 seconds @22:18:37.09 Evacuated: Intake @24.0psia, Outlet @23.4psia @22:18:37.10 SSV.dial :spit,avoiding: :exhaust @22:18:38.15 SS.reconfigure :exhale @22:18:38.20 SS.seek 0ml @22:18:43.27 SS.reconfigure :fast @22:18:43.32 SS.seek 5ml @22:18:46.29 SSV.dial CBV,avoiding: :exhaust @22:18:46.31
CBV.dial SSV @22:18:47.66 CTV.dial :air,avoiding: :intake @22:18:48.97 CSV.dial CTV @22:18:49.01
SS.seek 10ml @22:18:50.21 CSV.dial :air @22:18:50.66 CS.reconfigure :fast @22:18:51.14 CS.seek 0ml @22:18:51.63
SSV.dial :spit,avoiding: :exhaust @22:18:52.96 SS.reconfigure :exhale @22:18:53.01 SS.seek 0ml @22:18:57.84 CS.stop @22:18:58.01
SS.reconfigure :fast @22:18:58.06 SS.seek 5ml @22:19:00.90 SSV.dial CBV,avoiding: :exhaust @22:19:00.92
CBV.dial SSV @22:19:03.26 SSV.dial :spit,avoiding: :exhaust @22:19:04.59 SS.reconfigure :default @22:19:04.63 SS.seek 0ml @22:19:07.92 SS.stop @22:19:07.96 Lysing: Adding 2.0ml AqMeOH @22:19:07.98 CBV.dial between CSV and waste1,avoiding: :DBV @22:19:09.47 CTV.dial CSV @22:19:10.78 CSV.dial CSR @22:19:11.58 Pulling 1ml of CSR.AqMeOH CSR.select :AqMeOH @22:19:11.70 CS.reconfigure :default @22:19:11.75 CS.seek 1ml @22:19:15.35 delay 5 seconds @22:19:20.37 CSR.select :air @22:19:20.54 CS.seek 6ml @22:19:34.16 delay 2 seconds @22:19:36.17 CSV.dial :waste2 @22:19:36.63 CS.reconfigure :fast @22:19:36.68 CS.seek 0ml @22:19:51.09 CSV.dial CSR @22:19:51.67 Pulling 2ml of CSR.AqMeOH @22:19:51.68 CSR.select :AqMeOH @22:19:51.79 CS.reconfigure :default @22:19:51.84 CS.seek 2ml @22:19:57.85 delay 5 seconds @22:20:02.86 CSR.select :air @22:20:03.03 CS.seek 5ml @22:20:11.53 delay 2 seconds @22:20:13.55 CSV.dial CTV @22:20:14.02 CS.seek 1ml @22:20:25.23 Before lysing Collection Puck Heater at 12.7C/31.0psia @22:20:25.25 Heating Puck.from 5, :sh1 in Collection Clamp to 70C for 10:00 @22:20:25.27 CH.seek 70C,8:00 @22:22:40.56 CH.hold 10:00,70C @22:22:40.76 Begun lysing Collection Puck Heater at 68.1C/37.7psia @22:32:40.78 Ended lysing Collection Puck Heater at 70C/37.1psia @22:32:40.79 CS.seek 0ml @22:32:44.43 CTV.dial between spare and air,avoiding: :intake @22:32:45.46 CSV.dial :air @22:32:45.90 CS.seek 5ml @22:32:59.61 CSV.dial CTV @22:33:00.07 delay 2 seconds @22:33:02.09 CSV.dial :air @22:33:02.54 delay 2 seconds @22:33:04.55 CSV.dial CTV @22:33:05.01 delay 2 seconds @22:33:07.02 CSV.dial :air @22:33:07.48 delay 2 seconds @22:33:09.50 CS.seek 0ml @22:33:23.36 Puck @82.7psia after final lysis pressurization @22:33:23.37 Recovering Lysate into Collection syringe @22:33:23.38 CSV.dial CBV @22:33:24.40 CBV.dial CSV @22:33:24.77 CS.seek 5ml @22:33:38.36 delay 5 seconds @22:33:43.38 CTV.dial :air @22:33:43.90 CS.seek 7.001ml @22:33:49.80 delay 5 seconds @22:33:54.82 CSV.dial :air2 @22:33:55.26 CS.seek 10ml @22:34:03.74 CSV.dial between air and CTV,avoiding: CBV @22:34:04.90 Lysing Complete @22:34:04.97 Storing sh1 puck in Collection Clamp to tube 4 @22:34:05.15 SC.reconfigure :tight @22:34:05.20 SC.seek 4 @22:34:08.77 SC.configure :limp @22:34:08.99 Elbow.reconfigure :tight @22:34:09.04 Elbow.seek 4 @22:34:10.83 Forearm.reconfigure :tight @22:34:10.88 Forearm.seek 4 + 450 counts @22:34:11.91 SE.reconfigure :pick @22:34:11.97 SE.seek :beamBreak @22:34:14.65 SE.configure :default @22:34:15.25 Elbow.reconfigure :limp @22:34:15.30 Forearm.reconfigure :loose @22:34:15.35 Forearm.seek 4 @22:34:15.63 Elbow.hold @22:34:15.70 Elbow.reconfigure :tight @22:34:15.80 SE.seek 8.16 Pucks below Top Plate @22:34:16.19 Forearm.force -20 @22:34:16.24 Forearm.seek 4 + 279 counts @22:34:16.51 Forearm.reconfigure :tight @22:34:16.56 Forearm.seek 4 + 450 counts @22:34:16.87 SE.seek 9.16 Pucks below Top Plate @22:34:17.40 Forearm.reconfigure :loose @22:34:17.45 Forearm.seek :retract @22:34:18.43 Elbow.seek CC @22:34:20.12 Elbow.reconfigure :loose @22:34:20.22 Forearm.seek CC @22:34:21.17 Hand.close @22:34:21.81 CC.seek :open @22:34:26.53 Forearm.seek :retract @22:34:27.54 Elbow.reconfigure :tight @22:34:27.59 Elbow.seek 4 @22:34:29.36 Elbow.reconfigure :limp @22:34:29.41 Forearm.seek 4 @22:34:30.44 Elbow.hold @22:34:30.51 Elbow.reconfigure :tight @22:34:30.56 Hand.open @22:34:31.31 Forearm.force -20 @22:34:31.35 Forearm.seek 4 + 308 counts @22:34:32.12 Forearm.reconfigure :tight @22:34:32.23 Forearm.seek 4 + 450 counts @22:34:32.57 SE.seek :down @22:34:33.70
Forearm.reconfigure :loose @22:34:33.75 Forearm.seek :retract @22:34:34.66 SE.stop @22:34:34.72
Tranferring DA @22:34:34.73 Beginning samplePassC @22:34:34.75 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @22:34:34.93 Elbow.seek FlushPuck::Garage @22:34:36.77 Elbow.reconfigure :loose @22:34:36.87 Forearm.seek :garage @22:34:37.83 Hand.close @22:34:38.54 Forearm.seek :retract @22:34:39.51 Elbow.reconfigure :tight @22:34:39.56 Elbow.seek CC @22:34:42.48 Elbow.reconfigure :loose @22:34:42.59 Forearm.reconfigure :tight @22:34:42.64 Forearm.seek CC @22:34:43.88 CC.seek :closed @22:34:48.40 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.38 @22:34:48.47 Hand.open @22:34:49.11 Forearm.reconfigure :loose @22:34:49.16 Forearm.seek :retract @22:34:50.59 PSV.dial PSR @22:34:51.99 Pulling 1.5ml of PSR.flush PSR.select :flush @22:34:52.12 PS.seek 1.5ml @22:35:08.24 delay 5 seconds @22:35:13.30 PSV.dial :waste1 @22:35:13.76 PS.seek 0ml @22:35:30.08 PSV.dial PSR @22:35:30.55 PSR.select :air @22:35:30.67 PS.seek 2.5ml @22:35:57.09 PRV.dial CBV,avoiding: :ab2 @22:35:57.11
CBV.dial PRV @22:35:58.88 PSV.dial :mixloop @22:35:59.93 CTV.dial :air,avoiding: :intake @22:36:00.00 PTV.dial :PRVmixing @22:36:01.34 PS.seek 0ml @22:36:27.89 PSV.dial :air @22:36:28.48 PS.seek 2.5ml @22:36:54.80 PSV.dial :mixloop @22:36:55.26 PS.seek 0ml @22:37:21.58 PSV.dial :air @22:37:22.06 PS.seek 2.5ml @22:37:48.42 PSV.dial :mixloop @22:37:48.90 PS.seek 0ml @22:38:15.24 Garaging FlushPuck in Collection Clamp @22:38:15.31 Elbow.reconfigure :tight @22:38:15.36 Elbow.seek CC @22:38:15.68 Elbow.reconfigure :loose @22:38:15.78 Forearm.seek CC @22:38:16.81 Hand.close @22:38:17.43 CC.seek :open @22:38:22.05 Forearm.seek :retract @22:38:23.04 Elbow.reconfigure :tight @22:38:23.08 Elbow.seek :garage @22:38:26.01 Elbow.reconfigure :loose @22:38:26.11 Forearm.reconfigure :tight @22:38:26.16 Forearm.seek :garage @22:38:27.63 Hand.open @22:38:28.36 Forearm.reconfigure :loose @22:38:28.41 Forearm.seek :retract @22:38:29.44 PSV.dial :mixloop @22:38:29.52 PTV.dial :PRVmixing @22:38:29.60 PRV.dial CBV @22:38:29.62
CBV.dial PRV @22:38:29.86 CTV.dial :air,avoiding: :intake @22:38:29.94 Loading lyfil puck from tube 5 into Collection Clamp @22:38:30.18 SC.reconfigure :tight @22:38:30.23 SC.seek 5 @22:38:33.76 SC.configure :limp @22:38:33.98 Elbow.reconfigure :tight @22:38:34.03 Elbow.seek 5 @22:38:35.96 Forearm.reconfigure :tight @22:38:36.02 Forearm.seek 5 + 450 counts @22:38:37.05 SE.reconfigure :pick @22:38:37.11 SE.seek :beamBreak @22:38:38.69 SE.configure :default @22:38:38.86 Elbow.seek 5 @22:38:39.05 Forearm.seek 5 + 300 counts @22:38:39.28 Forearm.reconfigure :loose @22:38:39.33 Hand.close @22:38:39.94 SE.seek :down @22:38:40.15
Forearm.seek :retract @22:38:41.10 Elbow.seek CC @22:38:41.21 SE.stop @22:38:43.14
Elbow.reconfigure :loose @22:38:43.28 Forearm.reconfigure :tight @22:38:43.33 Forearm.seek CC @22:38:44.65 CC.seek :closed @22:38:49.18 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.19 @22:38:49.19 Hand.open @22:38:49.87 Forearm.reconfigure :loose @22:38:49.92 Forearm.seek :retract @22:38:50.93 CTV.dial CSV,avoiding: :intake @22:38:52.28 CSV.dial CTV @22:38:52.73 PS.seek 2.5ml @22:39:19.08 CS.seek 4.5ml @22:39:34.01 delay 8 seconds @22:39:42.04 PSV.dial :air @22:39:42.50 delay 2 seconds @22:39:44.52 PSV.dial :waste2 @22:39:45.38 PS.seek 0ml @22:40:11.73 CBV.dial between PRV and waste1 @22:40:12.06 CSV.dial :air @22:40:12.50 CS.seek 10ml @22:40:27.45 CSV.dial CTV @22:40:27.91 CS.seek 0ml @22:40:54.26 CBV.dial :waste1 @22:40:54.58 delay 5 seconds @22:40:59.60 CTV.dial :air,avoiding: :intake @22:41:01.01 Storing lyfil puck in Collection Clamp to tube 4 @22:41:01.19 SC.reconfigure :tight @22:41:01.24 SC.seek 4 @22:41:04.78 SC.configure :limp @22:41:05.45 Elbow.reconfigure :tight @22:41:05.49 Elbow.seek 4 @22:41:07.28 Forearm.reconfigure :tight @22:41:07.33 Forearm.seek 4 + 450 counts @22:41:08.37 SE.reconfigure :pick @22:41:08.43 SE.seek :beamBreak @22:41:10.94 SE.configure :default @22:41:11.10 Elbow.reconfigure :limp @22:41:11.15 Forearm.reconfigure :loose @22:41:11.20 Forearm.seek 4 @22:41:11.48 Elbow.hold @22:41:11.55 Elbow.reconfigure :tight @22:41:11.65 SE.seek 9.17 Pucks below Top Plate @22:41:12.05 Forearm.force -20 @22:41:12.09 Forearm.seek 4 + 301 counts @22:41:12.38 Forearm.reconfigure :tight @22:41:12.43 Forearm.seek 4 + 450 counts @22:41:12.65 SE.seek 10.17 Pucks below Top Plate @22:41:13.26 Forearm.reconfigure :loose @22:41:13.31 Forearm.seek :retract @22:41:14.25 Elbow.seek CC @22:41:15.96 Elbow.reconfigure :loose @22:41:16.06 Forearm.seek CC @22:41:17.01 Hand.close @22:41:17.67 CC.seek :open @22:41:22.41 Forearm.seek :retract @22:41:23.80 Elbow.reconfigure :tight @22:41:23.85 Elbow.seek 4 @22:41:25.62 Elbow.reconfigure :limp @22:41:25.67 Forearm.seek 4 @22:41:26.65 Elbow.hold @22:41:26.72 Elbow.reconfigure :tight @22:41:26.77 Hand.open @22:41:27.55 Forearm.force -20 @22:41:27.60 Forearm.seek 4 + 304 counts @22:41:28.01 Forearm.reconfigure :tight @22:41:28.06 Forearm.seek 4 + 450 counts @22:41:28.32 SE.seek :down @22:41:29.50
Forearm.reconfigure :loose @22:41:29.55 Forearm.seek :retract @22:41:30.37 SE.stop @22:41:30.47
PSV.dial :air @22:41:31.21 PS.seek 2.5ml @22:41:57.61 delay 1 second @22:41:58.62 PSV.dial :mixloop @22:41:59.08 delay 1 second @22:42:00.09 PSV.dial :air @22:42:00.56 delay 1 second @22:42:01.58 PSV.dial :mixloop @22:42:02.05 delay 1 second @22:42:03.06 PSV.dial :air @22:42:03.53 delay 1 second @22:42:04.55 PS.seek 0ml @22:42:30.92 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @22:42:31.11 Elbow.seek FlushPuck::Garage @22:42:32.88 Elbow.reconfigure :loose @22:42:32.98 Forearm.seek :garage @22:42:34.00 Hand.close @22:42:35.21 Forearm.seek :retract @22:42:36.22 Elbow.reconfigure :tight @22:42:36.26 Elbow.seek PC @22:42:37.56 Elbow.reconfigure :loose @22:42:37.66 Forearm.reconfigure :tight @22:42:37.71 Forearm.seek PC @22:42:39.01 PC.seek :closed @22:42:43.65 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.68 @22:42:43.72 Hand.open @22:42:44.36 Forearm.reconfigure :loose @22:42:44.40 Forearm.seek :retract @22:42:45.36 PRV.dial :air @22:42:45.81 PSV.dial :mixloop @22:42:46.35 PS.seek 0.3ml @22:42:50.26 PSV.dial PSR @22:42:51.33 Pulling 0.4ml of PSR.air @22:42:51.34 PSR.select :air @22:42:51.37 PS.seek 0.7ml @22:42:56.26 delay 3 seconds @22:42:59.28 PSR.select :air @22:42:59.31 PS.seek 2.5ml @22:43:18.70 delay 3 seconds @22:43:21.72 PSV.dial :mixloop @22:43:22.78 PTV.dial :mixing @22:43:23.53 PBV.dial :waste2 @22:43:24.49 PS.seek 0ml @22:43:50.83 Garaging FlushPuck in Processing Clamp @22:43:50.90 Elbow.reconfigure :tight @22:43:50.95 Elbow.seek PC @22:43:51.06 Elbow.reconfigure :loose @22:43:51.17 Forearm.seek PC @22:43:52.55 Hand.close @22:43:53.17 PC.seek :open @22:43:57.88 Forearm.seek :retract @22:43:58.86 Elbow.reconfigure :tight @22:43:58.91 Elbow.seek :garage @22:44:00.37 Elbow.reconfigure :loose @22:44:00.47 Forearm.reconfigure :tight @22:44:00.52 Forearm.seek :garage @22:44:01.56 Hand.open @22:44:02.23 Forearm.reconfigure :loose @22:44:02.28 Forearm.seek :retract @22:44:03.25 Loading da puck from tube 5 into Processing Clamp @22:44:03.49 SC.reconfigure :tight @22:44:03.54 SC.seek 5 @22:44:07.07 SC.configure :limp @22:44:07.29 Elbow.reconfigure :tight @22:44:07.34 Elbow.seek 5 @22:44:09.32 Forearm.reconfigure :tight @22:44:09.37 Forearm.seek 5 + 450 counts @22:44:10.53 SE.reconfigure :pick @22:44:10.58 SE.seek :beamBreak @22:44:12.26 SE.configure :default @22:44:12.42 Elbow.seek 5 @22:44:12.61 Forearm.seek 5 + 300 counts @22:44:12.76 Forearm.reconfigure :loose @22:44:12.81 Hand.close @22:44:13.43 SE.seek :down @22:44:13.64
Forearm.seek :retract @22:44:14.58 Elbow.seek PC @22:44:15.09 SE.stop @22:44:17.62
Elbow.reconfigure :loose @22:44:17.72 Forearm.reconfigure :tight @22:44:17.77 Forearm.seek PC @22:44:19.06 PC.seek :closed @22:44:23.73 Processing Clamp onSpring/offSpring for 16/26 samples, on/off torque 1.66 @22:44:23.74 Hand.open @22:44:24.36 Forearm.reconfigure :loose @22:44:24.40 Forearm.seek :retract @22:44:25.37 Heating Puck.from 5, :da in Processing Clamp to 28C for 2:00:00 @22:44:25.38 PH.seek 28C,5:00 @22:45:09.16 PH.hold 2:00:00,28C @22:45:09.21 delay 1:30 @22:46:39.26 PSR.select :air @22:46:39.28 PSV.dial PSR @22:46:40.34 Pulling 0.5ml of PSR.wash @22:46:40.35 PSR.select :wash @22:46:40.47 PS.seek 0.5ml @22:46:46.45 delay 5 seconds @22:46:51.47 PSR.select :air @22:46:51.59 PS.seek 2ml @22:47:07.73 PTV.dial :puck @22:47:08.48 PSV.dial PTV @22:47:09.37 PBV.dial :waste1 @22:47:10.77 PS.seek 1.5ml @22:47:16.76 delay 3 seconds @22:47:19.78 PBV.dial between PSV and waste1 @22:47:20.14 PS.seek 0ml @22:47:36.20 delay 5:00 @22:52:36.22 recoverPSRreagent08 PTV.dial :puck @22:52:36.23 PBV.dial :waste1 @22:52:36.61 delay 30 seconds @22:53:06.64 PBV.dial between PSV and waste1 @22:53:07.00 PSR.select :air @22:53:07.02 PSV.dial PSR @22:53:07.77 PS.reconfigure :fast @22:53:07.82 PS.seek 2.5ml @22:53:31.53 PSV.dial PTV @22:53:32.30 delay 5 seconds @22:53:37.32 PSV.dial PSR @22:53:38.07 delay 5 seconds @22:53:43.09 PSV.dial PTV @22:53:43.85 delay 5 seconds @22:53:48.87 PSV.dial :air @22:53:49.65 PS.seek 0ml @22:54:12.78 PTV.dial :PRVmixing,via: PRV @22:54:14.11 PSV.dial :mixloop @22:54:14.59 PRV.dial :ab1 @22:54:15.35 PS.reconfigure :slow @22:54:15.40 PS.seek 0.15ml @22:54:19.03 delay 2 seconds @22:54:21.05 CBV.dial PRV @22:54:21.08
PRV.dial CBV @22:54:22.10 PS.seek 0.35ml @22:54:26.72 delay 2 seconds @22:54:28.74 CBV.dial between PRV and PSV @22:54:29.06 PRV.dial :air @22:54:29.54 PS.seek 0.9ml @22:54:41.22 PRV.dial :waste2 @22:54:41.98 PS.seek 0.5ml @22:54:50.69 addPMLReagent08 @22:54:50.70 PSV.dial :air @22:54:51.16 PS.reconfigure :default @22:54:51.21 PS.seek 2ml @22:55:07.40 PSV.dial :PML @22:55:07.88 PTV.dial :mixing @22:55:08.63 PBV.dial :waste1 @22:55:09.06 PS.seek 1.85ml @22:55:11.50 PBV.dial between PSV and waste1 @22:55:11.88 PS.seek 0ml @22:55:31.56 delay 2 seconds @22:55:33.58 Beginning samplePassC @22:55:33.59 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @22:55:33.78 Elbow.reconfigure :tight @22:55:33.83 Elbow.seek FlushPuck::Garage @22:55:35.27 Elbow.reconfigure :loose @22:55:35.37 Forearm.seek :garage @22:55:36.33 Hand.close @22:55:37.03 Forearm.seek :retract @22:55:37.99 Elbow.reconfigure :tight @22:55:38.04 Elbow.seek CC @22:55:41.00 Elbow.reconfigure :loose @22:55:41.10 Forearm.reconfigure :tight @22:55:41.15 Forearm.seek CC @22:55:42.35 CC.seek :closed @22:55:46.88 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.44 @22:55:46.95 Hand.open @22:55:47.60 Forearm.reconfigure :loose @22:55:47.65 Forearm.seek :retract @22:55:48.60 PSV.dial PSR @22:55:49.66 Pulling 1.5ml of PSR.flush PSR.select :flush @22:55:49.79 PS.seek 1.5ml @22:56:05.87 delay 5 seconds @22:56:10.89 PSV.dial :waste1 @22:56:11.36 PS.seek 0ml @22:56:27.53 PSV.dial PSR @22:56:28.00 PSR.select :air @22:56:28.12 PS.seek 2.5ml @22:56:54.44 PRV.dial CBV,avoiding: :ab2 @22:56:54.46
CBV.dial PRV @22:56:56.09 PSV.dial :mixloop @22:56:57.14 CTV.dial :air,avoiding: :intake @22:56:57.22 PTV.dial :PRVmixing @22:56:58.00 PS.seek 0ml @22:57:24.31 PSV.dial :air @22:57:24.79 PS.seek 2.5ml @22:57:51.18 PSV.dial :mixloop @22:57:51.68 PS.seek 0ml @22:58:18.06 PSV.dial :air @22:58:18.53 PS.seek 2.5ml @22:58:44.87 PSV.dial :mixloop @22:58:45.35 PS.seek 0ml @22:59:11.65 Garaging FlushPuck in Collection Clamp @22:59:11.72 Elbow.reconfigure :tight @22:59:11.77 Elbow.seek CC @22:59:12.08 Elbow.reconfigure :loose @22:59:12.18 Forearm.seek CC @22:59:13.22 Hand.close @22:59:14.16 CC.seek :open @22:59:18.87 Forearm.seek :retract @22:59:19.86 Elbow.reconfigure :tight @22:59:19.91 Elbow.seek :garage @22:59:22.81 Elbow.reconfigure :loose @22:59:22.91 Forearm.reconfigure :tight @22:59:22.96 Forearm.seek :garage @22:59:23.97 Hand.open @22:59:24.59 Forearm.reconfigure :loose @22:59:24.64 Forearm.seek :retract @22:59:25.63 delayUntil "23:03:33.57" @22:59:25.66 Whole Cell Archive sampling 1000ml @22:59:25.67 Loading wcr puck from tube 5 into Collection Clamp @22:59:26.16 Elbow.reconfigure :tight @22:59:26.21 Elbow.seek 5 @22:59:28.11 Forearm.reconfigure :tight @22:59:28.16 Forearm.seek 5 + 450 counts @22:59:29.20 SE.reconfigure :pick @22:59:29.26 SE.seek :beamBreak @22:59:31.05 SE.configure :default @22:59:31.22 Elbow.seek 5 @22:59:31.41 Forearm.seek 5 + 300 counts @22:59:31.61 Forearm.reconfigure :loose @22:59:31.66 Hand.close @22:59:32.30 SE.seek :down @22:59:32.94 Forearm.seek :retract @22:59:33.70 SE.stop @22:59:33.91 Elbow.seek CC @22:59:35.50 Elbow.reconfigure :loose @22:59:35.60 Forearm.reconfigure :tight @22:59:35.66 Forearm.seek CC @22:59:36.92 CC.seek :closed @22:59:41.44 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.41 @22:59:41.45 Hand.open @22:59:42.10 Forearm.reconfigure :loose @22:59:42.15 Forearm.seek :retract @22:59:43.13 collectSample 1000ml w/bubble point of 35psi @22:59:43.14 Priming 7ml @22:59:43.15 Intake.select :close @22:59:43.16 Exhaust.select :close @22:59:43.18 SS.hold @22:59:43.39 Calibrating pressure sensors @22:59:43.40 CTV.dial :air @22:59:43.48 SSV.dial :spit,avoiding: :exhaust @22:59:43.57 SS.reconfigure :fast @22:59:43.62 SS.seek 5ml @22:59:46.33 SSV.dial CBV @22:59:46.38 CBV.dial SSV @22:59:47.71 delay 3.8 seconds @22:59:51.65 Sensor limits: Intake max 103.6psia; Outlet max 94.4psia @22:59:51.66 SSV.dial :spit @22:59:52.99 SS.seek 0ml @22:59:55.88 SSV.dial CBV @22:59:57.22 CTV.dial :intake @22:59:58.44 SSV.dial CBV @22:59:58.47 CBV.dial SSV @22:59:58.61 Allowing 40s for 7.0ml of remaining 7.0ml @22:59:58.63 Intake.select :open @23:00:00.80 Intake @14.0psia, vacuum from 8.5 to 11.5psi @23:00:00.82 SS.reconfigure :inhaling 2.20ml/s @23:00:00.87 SS.seek 7ml @23:00:07.72 Stroke completed -- waiting for pressure to equalize ... @23:00:07.73 SS.reconfigure :exhale @23:00:07.78 SS.hold @23:00:22.06 Intake.select :close @23:00:22.22 SSV.dial :exhaust @23:00:23.00 Exhaust.select :open @23:00:23.12 SS.seek 0ml @23:00:27.66 Exhaust.select :close @23:00:27.77 Sampling 1000.0ml @23:00:27.79 Intake.select :close @23:00:27.80 Exhaust.select :close @23:00:27.81 SS.reconfigure :default @23:00:27.86 SS.hold @23:00:28.08 CTV.dial :intake @23:00:28.26 SSV.dial CBV @23:00:28.29 CBV.dial SSV @23:00:29.09 Allowing 130s for 25.0ml of remaining 1000.0ml @23:00:29.11 Intake.select :open @23:00:31.28 Intake @15.1psia, vacuum from 9.6 to 12.6psi @23:00:31.30 SS.reconfigure :inhaling 2.20ml/s @23:00:31.35 SS.seek 25ml @23:00:38.82 Vacuum too high -- waiting up to 40 seconds @23:00:38.84 SS.reconfigure :exhale @23:00:38.89 SS.hold @23:00:46.77 Intake @18.3psia, vacuum from 10.0 to 13.0psi @23:00:46.79 SS.reconfigure :inhaling 1.76ml/s @23:00:46.83 SS.seek 25ml @23:00:49.45 Vacuum too high -- waiting up to 40 seconds @23:00:49.47 SS.reconfigure :exhale @23:00:49.53 SS.hold @23:00:51.45 Intake @17.3psia, vacuum from 10.0 to 13.0psi @23:00:51.47 SS.reconfigure :inhaling 1.41ml/s @23:00:51.52 SS.seek 25ml @23:00:55.45 Vacuum too high -- waiting up to 40 seconds @23:00:55.46 SS.reconfigure :exhale @23:00:55.52 SS.hold @23:00:59.20 Intake @18.6psia, vacuum from 10.0 to 13.0psi @23:00:59.22 SS.reconfigure :inhaling 1.13ml/s @23:00:59.27 SS.seek 25ml @23:01:01.95 Vacuum too high -- waiting up to 40 seconds @23:01:01.96 SS.reconfigure :exhale @23:01:02.02 SS.hold @23:01:04.47 Intake @18.6psia, vacuum from 10.0 to 13.0psi @23:01:04.49 SS.reconfigure :inhaling 0.91ml/s @23:01:04.54 SS.seek 25ml @23:01:09.04 Vacuum too high -- waiting up to 40 seconds @23:01:09.05 SS.reconfigure :exhale @23:01:09.11 SS.hold @23:01:11.11 Intake @18.4psia, vacuum from 10.0 to 13.0psi @23:01:11.13 SS.reconfigure :inhaling 0.73ml/s @23:01:11.18 SS.seek 25ml @23:01:12.23 Stroke completed -- waiting for pressure to equalize ... @23:01:12.24 SS.reconfigure :exhale @23:01:12.29 SS.hold @23:01:37.34 Intake.select :close @23:01:37.50 SSV.dial :exhaust @23:01:38.26 Exhaust.select :open @23:01:38.38 SS.seek 0ml @23:01:47.42 Exhaust.select :close @23:01:47.53 SSV.dial CBV @23:01:47.56 CBV.dial SSV @23:01:48.37 Allowing 130s for 25.0ml of remaining 975.0ml @23:01:48.39 Intake.select :open @23:01:50.55 Intake @15.6psia, vacuum from 10.0 to 13.0psi @23:01:50.57 SS.reconfigure :inhaling 0.73ml/s @23:01:50.62 SS.seek 25ml @23:02:26.43 Stroke completed -- waiting for pressure to equalize ... @23:02:26.45 SS.reconfigure :exhale @23:02:26.50 SS.hold @23:02:51.01 Intake.select :close @23:02:51.17 SSV.dial :exhaust @23:02:51.95 Exhaust.select :open @23:02:52.07 SS.seek 0ml @23:03:00.91 Exhaust.select :close @23:03:01.03 SSV.dial CBV @23:03:01.05 CBV.dial SSV @23:03:01.86 Allowing 130s for 25.0ml of remaining 950.0ml @23:03:01.87 Intake.select :open @23:03:04.04 Intake @15.6psia, vacuum from 10.0 to 13.0psi @23:03:04.06 SS.reconfigure :inhaling 0.73ml/s @23:03:04.12 SS.seek 25ml @23:03:33.58 recoverPRVreagent08 @23:03:33.60 PTV.dial :mixing @23:03:34.37 PBV.dial :waste1 @23:03:34.74 delay 30 seconds @23:03:40.76 Stroke completed -- waiting for pressure to equalize ... @23:03:40.77 SS.reconfigure :exhale @23:03:40.82 SS.hold @23:04:04.76 PBV.dial between PSV and waste1 @23:04:05.13 PSV.dial :air @23:04:05.79 PS.reconfigure :fast @23:04:05.89 Intake.select :close @23:04:05.90 PS.seek 2.5ml @23:04:06.09 SSV.dial :exhaust @23:04:06.86 Exhaust.select :open @23:04:06.98 SS.seek 0ml @23:04:15.82 Exhaust.select :close @23:04:15.93 SSV.dial CBV @23:04:15.98 CBV.dial SSV @23:04:16.73 Allowing 130s for 25.0ml of remaining 925.0ml @23:04:16.74 Intake.select :open @23:04:18.91 Intake @15.6psia, vacuum from 10.0 to 13.0psi @23:04:18.93 SS.reconfigure :inhaling 0.73ml/s @23:04:18.98 SS.seek 25ml @23:04:29.99 PSV.dial :mixloop @23:04:30.44 delay 2 seconds @23:04:32.46 PSV.dial :air @23:04:32.93 delay 2 seconds @23:04:34.95 PSV.dial :mixloop @23:04:35.42 delay 2 seconds @23:04:37.44 PSV.dial :air @23:04:37.90 PS.seek 0ml @23:04:55.68 Stroke completed -- waiting for pressure to equalize ... @23:04:55.69 SS.reconfigure :exhale @23:04:55.75 SS.hold @23:05:01.27 washCycleDA #1 of 2 @23:05:01.28 PSR.select :air @23:05:01.30 PSV.dial PSR @23:05:02.66 Pulling 0.5ml of PSR.wash @23:05:02.67 PSR.select :wash @23:05:02.79 PS.reconfigure :default @23:05:02.84 PS.seek 0.5ml @23:05:08.71 delay 5 seconds @23:05:13.72 PSR.select :air @23:05:13.91 PS.seek 2ml @23:05:18.35 Intake.select :close @23:05:18.51 SSV.dial :exhaust @23:05:19.28 Exhaust.select :open @23:05:19.40 SS.seek 0ml @23:05:28.20 Exhaust.select :close @23:05:28.31 SSV.dial CBV @23:05:28.33 CBV.dial SSV @23:05:29.13 Allowing 130s for 25.0ml of remaining 900.0ml @23:05:29.15 Intake.select :open @23:05:30.14 PTV.dial :puck @23:05:30.90 PSV.dial PTV @23:05:31.32 Intake @15.6psia, vacuum from 10.0 to 13.0psi @23:05:31.34 SS.reconfigure :inhaling 0.73ml/s @23:05:31.39 SS.seek 25ml @23:05:31.65 PBV.dial :waste1 @23:05:32.08 PS.seek 1.5ml @23:05:38.02 delay 3 seconds @23:05:41.04 PBV.dial between PSV and waste1 @23:05:41.40 PS.seek 0ml @23:05:57.49 delay 2:00 @23:06:07.90 Stroke completed -- waiting for pressure to equalize ... @23:06:07.91 SS.reconfigure :exhale @23:06:07.96 SS.hold @23:06:28.28 Intake.select :close @23:06:28.44 SSV.dial :exhaust @23:06:29.20 Exhaust.select :open @23:06:29.32 SS.seek 0ml @23:06:38.20 Exhaust.select :close @23:06:38.31 SSV.dial CBV @23:06:38.34 CBV.dial SSV @23:06:39.13 Allowing 130s for 25.0ml of remaining 875.0ml @23:06:39.15 Intake.select :open @23:06:41.32 Intake @15.5psia, vacuum from 10.0 to 13.0psi @23:06:41.34 SS.reconfigure :inhaling 0.73ml/s @23:06:41.39 SS.seek 25ml @23:07:17.90 Stroke completed -- waiting for pressure to equalize ... @23:07:17.91 SS.reconfigure :exhale @23:07:17.96 SS.hold @23:07:36.45 Intake.select :close @23:07:36.61 SSV.dial :exhaust @23:07:37.38 Exhaust.select :open @23:07:37.50 SS.seek 0ml @23:07:46.52 Exhaust.select :close @23:07:46.63 SSV.dial CBV @23:07:46.67 CBV.dial SSV @23:07:47.43 Allowing 130s for 25.0ml of remaining 850.0ml @23:07:47.44 Intake.select :open @23:07:49.61 Intake @15.6psia, vacuum from 10.0 to 13.0psi @23:07:49.63 SS.reconfigure :inhaling 0.73ml/s @23:07:49.68 SS.seek 25ml @23:07:57.51 recoverPSRreagent08 @23:07:57.52 PTV.dial :puck @23:07:57.60 PBV.dial :waste1 @23:07:57.97 delay 30 seconds @23:08:25.77 Stroke completed -- waiting for pressure to equalize ... @23:08:25.78 SS.reconfigure :exhale @23:08:25.83 SS.hold @23:08:28.05 PBV.dial between PSV and waste1 @23:08:28.44 PSR.select :air @23:08:28.50 PSV.dial PSR @23:08:29.41 PS.reconfigure :fast @23:08:29.46 PS.seek 2.5ml @23:08:44.45 Intake.select :close @23:08:44.61 SSV.dial :exhaust @23:08:45.38 Exhaust.select :open @23:08:45.50 SS.seek 0ml @23:08:53.36 PSV.dial PTV @23:08:54.11 delay 5 seconds @23:08:54.33 Exhaust.select :close @23:08:54.45 SSV.dial CBV @23:08:54.48 CBV.dial SSV @23:08:55.26 Allowing 130s for 25.0ml of remaining 825.0ml @23:08:55.27 Intake.select :open @23:08:57.44 Intake @15.6psia, vacuum from 10.0 to 13.0psi @23:08:57.46 SS.reconfigure :inhaling 0.73ml/s @23:08:57.51 SS.seek 25ml @23:08:59.13 PSV.dial PSR @23:08:59.89 delay 5 seconds @23:09:04.91 PSV.dial PTV @23:09:05.69 delay 5 seconds @23:09:10.71 PSV.dial :air @23:09:11.49 PS.seek 0ml @23:09:33.86 Stroke completed -- waiting for pressure to equalize ... @23:09:33.88 SS.reconfigure :exhale @23:09:33.93 SS.hold @23:09:34.74 washCycleDA #2 of 2 @23:09:34.75 PSR.select :air @23:09:34.76 PSV.dial PSR @23:09:36.11 Pulling 0.5ml of PSR.wash @23:09:36.12 PSR.select :wash @23:09:36.24 PS.reconfigure :default @23:09:36.28 PS.seek 0.5ml @23:09:42.39 delay 5 seconds @23:09:47.41 PSR.select :air @23:09:47.59 PS.seek 2ml @23:09:52.39 Intake.select :close @23:09:52.55 SSV.dial :exhaust @23:09:53.32 Exhaust.select :open @23:09:53.44 SS.seek 0ml @23:10:02.22 Exhaust.select :close @23:10:02.33 SSV.dial CBV @23:10:02.36 CBV.dial SSV @23:10:03.15 Allowing 130s for 25.0ml of remaining 800.0ml @23:10:03.16 Intake.select :open @23:10:03.70 PTV.dial :puck @23:10:03.78 PSV.dial PTV @23:10:04.66 PBV.dial :waste1 @23:10:05.09 PS.seek 1.5ml @23:10:05.33 Intake @15.5psia, vacuum from 10.0 to 13.0psi @23:10:05.35 SS.reconfigure :inhaling 0.73ml/s @23:10:05.40 SS.seek 25ml @23:10:11.02 delay 3 seconds @23:10:14.04 PBV.dial between PSV and waste1 @23:10:14.41 PS.seek 0ml @23:10:30.77 delay 2:00 @23:10:42.10 Stroke completed -- waiting for pressure to equalize ... @23:10:42.11 SS.reconfigure :exhale @23:10:42.16 SS.hold @23:11:00.54 Intake.select :close @23:11:00.70 SSV.dial :exhaust @23:11:01.49 Exhaust.select :open @23:11:01.61 SS.seek 0ml @23:11:10.64 Exhaust.select :close @23:11:10.75 SSV.dial CBV @23:11:10.79 CBV.dial SSV @23:11:11.57 Allowing 130s for 25.0ml of remaining 775.0ml @23:11:11.58 Intake.select :open @23:11:13.75 Intake @15.4psia, vacuum from 9.9 to 12.9psi @23:11:13.77 SS.reconfigure :inhaling 0.73ml/s @23:11:13.82 SS.seek 25ml @23:11:49.72 Stroke completed -- waiting for pressure to equalize ... @23:11:49.73 SS.reconfigure :exhale @23:11:49.78 SS.hold @23:12:08.14 Intake.select :close @23:12:08.30 SSV.dial :exhaust @23:12:09.07 Exhaust.select :open @23:12:09.18 SS.seek 0ml @23:12:17.99 Exhaust.select :close @23:12:18.10 SSV.dial CBV @23:12:18.13 CBV.dial SSV @23:12:18.92 Allowing 130s for 25.0ml of remaining 750.0ml @23:12:18.94 Intake.select :open @23:12:21.10 Intake @15.6psia, vacuum from 10.0 to 13.0psi @23:12:21.12 SS.reconfigure :inhaling 0.73ml/s @23:12:21.17 SS.seek 25ml @23:12:30.79 recoverPSRreagent08 @23:12:30.80 PTV.dial :puck @23:12:30.87 PBV.dial :waste1 @23:12:31.25 delay 30 seconds @23:12:57.58 Stroke completed -- waiting for pressure to equalize ... @23:12:57.59 SS.reconfigure :exhale @23:12:57.64 SS.hold @23:13:01.27 PBV.dial between PSV and waste1 @23:13:01.64 PSR.select :air @23:13:01.66 PSV.dial PSR @23:13:02.42 PS.reconfigure :fast @23:13:02.47 PS.seek 2.5ml @23:13:16.31 Intake.select :close @23:13:16.47 SSV.dial :exhaust @23:13:17.24 Exhaust.select :open @23:13:17.36 SS.seek 0ml @23:13:26.17 Exhaust.select :close @23:13:26.28 SSV.dial CBV @23:13:26.30 CBV.dial SSV @23:13:26.67 PSV.dial PTV @23:13:27.10 Allowing 130s for 25.0ml of remaining 725.0ml @23:13:27.11 Intake.select :open @23:13:27.45 delay 5 seconds @23:13:29.74 Intake @15.5psia, vacuum from 10.0 to 13.0psi @23:13:29.76 SS.reconfigure :inhaling 0.73ml/s @23:13:29.81 SS.seek 25ml @23:13:32.47 PSV.dial PSR @23:13:33.23 delay 5 seconds @23:13:38.25 PSV.dial PTV @23:13:39.01 delay 5 seconds @23:13:44.03 PSV.dial :air @23:13:44.92 PS.seek 0ml @23:14:06.06 Stroke completed -- waiting for pressure to equalize ... @23:14:06.07 SS.reconfigure :exhale @23:14:06.13 SS.hold @23:14:08.29 getPMLReagent08 @23:14:08.30 PRV.dial :air @23:14:08.78 delay 5 seconds @23:14:13.79 PTV.dial :PRVmixing @23:14:15.14 delay 5 seconds @23:14:20.15 PRV.dial :ab2 @23:14:20.62 PTV.dial :PRVmixing @23:14:21.14 PSV.dial :mixloop @23:14:21.59 PS.reconfigure :default @23:14:21.64 PS.seek 0.3ml @23:14:25.54 delay 2 seconds @23:14:26.54 Intake.select :close @23:14:26.70 SSV.dial :exhaust @23:14:27.49 Exhaust.select :open @23:14:27.56 PRV.dial :air @23:14:27.61 SS.seek 0ml @23:14:28.09 PS.seek 0.55ml @23:14:31.45 addPMLReagent08 @23:14:31.46 PSV.dial :air @23:14:32.06 PS.seek 2ml @23:14:36.41 Exhaust.select :close @23:14:36.52 SSV.dial CBV @23:14:36.55 CBV.dial SSV @23:14:37.34 Allowing 130s for 25.0ml of remaining 700.0ml @23:14:37.36 Intake.select :open @23:14:39.53 Intake @15.5psia, vacuum from 10.0 to 13.0psi @23:14:39.55 SS.reconfigure :inhaling 0.73ml/s @23:14:39.60 SS.seek 25ml @23:14:47.67 PSV.dial :PML @23:14:48.12 PTV.dial :mixing @23:14:48.88 PBV.dial :waste1 @23:14:49.32 PS.seek 1.85ml @23:14:51.67 PBV.dial between PSV and waste1 @23:14:52.03 PS.seek 0ml @23:15:11.79 delay 2 seconds @23:15:13.81 delay 8:00 @23:15:16.09 Stroke completed -- waiting for pressure to equalize ... @23:15:16.10 SS.reconfigure :exhale @23:15:16.15 SS.hold @23:15:38.70 Intake.select :close @23:15:38.86 SSV.dial :exhaust @23:15:39.63 Exhaust.select :open @23:15:39.75 SS.seek 0ml @23:15:48.75 Exhaust.select :close @23:15:48.86 SSV.dial CBV @23:15:48.88 CBV.dial SSV @23:15:49.70 Allowing 130s for 25.0ml of remaining 675.0ml @23:15:49.72 Intake.select :open @23:15:51.88 Intake @15.4psia, vacuum from 9.9 to 12.9psi @23:15:51.90 SS.reconfigure :inhaling 0.73ml/s @23:15:51.95 SS.seek 25ml @23:16:28.07 Stroke completed -- waiting for pressure to equalize ... @23:16:28.09 SS.reconfigure :exhale @23:16:28.13 SS.hold @23:16:50.89 Intake.select :close @23:16:51.05 SSV.dial :exhaust @23:16:51.81 Exhaust.select :open @23:16:51.93 SS.seek 0ml @23:17:01.37 Exhaust.select :close @23:17:01.49 SSV.dial CBV @23:17:01.53 CBV.dial SSV @23:17:02.29 Allowing 130s for 25.0ml of remaining 650.0ml @23:17:02.31 Intake.select :open @23:17:04.94 Intake @15.3psia, vacuum from 9.8 to 12.8psi @23:17:04.96 SS.reconfigure :inhaling 0.73ml/s @23:17:05.01 SS.seek 25ml @23:17:40.79 Stroke completed -- waiting for pressure to equalize ... @23:17:40.80 SS.reconfigure :exhale @23:17:40.85 SS.hold @23:18:05.37 Intake.select :close @23:18:05.94 SSV.dial :exhaust @23:18:06.70 Exhaust.select :open @23:18:06.82 SS.seek 0ml @23:18:15.62 Exhaust.select :close @23:18:15.74 SSV.dial CBV @23:18:15.76 CBV.dial SSV @23:18:16.57 Allowing 130s for 25.0ml of remaining 625.0ml @23:18:16.59 Intake.select :open @23:18:18.75 Intake @15.4psia, vacuum from 9.9 to 12.9psi @23:18:18.77 SS.reconfigure :inhaling 0.73ml/s @23:18:18.82 SS.seek 25ml @23:18:55.24 Stroke completed -- waiting for pressure to equalize ... @23:18:55.26 SS.reconfigure :exhale @23:18:55.30 SS.hold @23:19:21.87 Intake.select :close @23:19:22.40 SSV.dial :exhaust @23:19:23.18 Exhaust.select :open @23:19:23.30 SS.seek 0ml @23:19:32.38 Exhaust.select :close @23:19:32.50 SSV.dial CBV @23:19:32.52 CBV.dial SSV @23:19:33.32 Allowing 130s for 25.0ml of remaining 600.0ml @23:19:33.34 Intake.select :open @23:19:35.50 Intake @15.5psia, vacuum from 10.0 to 13.0psi @23:19:35.52 SS.reconfigure :inhaling 0.73ml/s @23:19:35.57 SS.seek 25ml @23:20:11.48 Stroke completed -- waiting for pressure to equalize ... @23:20:11.49 SS.reconfigure :exhale @23:20:11.54 SS.hold @23:20:40.24 Intake.select :close @23:20:40.40 SSV.dial :exhaust @23:20:41.16 Exhaust.select :open @23:20:41.28 SS.seek 0ml @23:20:50.10 Exhaust.select :close @23:20:50.21 SSV.dial CBV @23:20:50.23 CBV.dial SSV @23:20:51.02 Allowing 130s for 25.0ml of remaining 575.0ml @23:20:51.04 Intake.select :open @23:20:53.20 Intake @15.4psia, vacuum from 9.9 to 12.9psi @23:20:53.22 SS.reconfigure :inhaling 0.73ml/s @23:20:53.27 SS.seek 25ml @23:21:27.02 Vacuum too high -- waiting up to 40 seconds @23:21:27.03 SS.reconfigure :exhale @23:21:27.09 SS.hold @23:21:44.04 Intake @16.8psia, vacuum from 10.0 to 13.0psi @23:21:44.06 SS.reconfigure :inhaling 0.59ml/s @23:21:44.11 SS.seek 25ml @23:21:47.57 Stroke completed -- waiting for pressure to equalize ... @23:21:47.58 SS.reconfigure :exhale @23:21:47.63 SS.hold @23:22:00.33 Intake.select :close @23:22:00.49 SSV.dial :exhaust @23:22:01.26 Exhaust.select :open @23:22:01.38 SS.seek 0ml @23:22:10.22 Exhaust.select :close @23:22:10.33 SSV.dial CBV @23:22:10.37 CBV.dial SSV @23:22:11.15 Allowing 130s for 25.0ml of remaining 550.0ml @23:22:11.16 Intake.select :open @23:22:13.34 Intake @15.5psia, vacuum from 10.0 to 13.0psi @23:22:13.36 SS.reconfigure :inhaling 0.59ml/s @23:22:13.41 SS.seek 25ml @23:22:58.18 Stroke completed -- waiting for pressure to equalize ... @23:22:58.19 SS.reconfigure :exhale @23:22:58.24 SS.hold @23:23:13.83 recoverPRVreagent08 @23:23:13.84 PTV.dial :mixing @23:23:13.91 PBV.dial :waste1 @23:23:14.28 delay 30 seconds @23:23:24.85 Intake.select :close @23:23:25.01 SSV.dial :exhaust @23:23:25.79 Exhaust.select :open @23:23:25.91 SS.seek 0ml @23:23:34.90 Exhaust.select :close @23:23:35.01 SSV.dial CBV @23:23:35.03 CBV.dial SSV @23:23:35.82 Allowing 130s for 25.0ml of remaining 525.0ml @23:23:35.83 Intake.select :open @23:23:38.00 Intake @15.5psia, vacuum from 10.0 to 13.0psi @23:23:38.02 SS.reconfigure :inhaling 0.59ml/s @23:23:38.07 SS.seek 25ml @23:23:44.34 PBV.dial between PSV and waste1 @23:23:44.72 PSV.dial :air @23:23:45.32 PS.reconfigure :fast @23:23:45.37 PS.seek 2.5ml @23:24:09.41 PSV.dial :mixloop @23:24:09.86 delay 2 seconds @23:24:11.88 PSV.dial :air @23:24:12.35 delay 2 seconds @23:24:14.36 PSV.dial :mixloop @23:24:14.84 delay 2 seconds @23:24:16.86 PSV.dial :air @23:24:17.32 PS.seek 0ml @23:24:22.61 Stroke completed -- waiting for pressure to equalize ... @23:24:22.63 SS.reconfigure :exhale @23:24:22.67 SS.hold @23:24:40.56 washCycleDA #1 of 3 @23:24:40.58 PSR.select :air @23:24:40.59 PSV.dial PSR @23:24:41.94 Pulling 0.5ml of PSR.wash @23:24:41.95 PSR.select :wash @23:24:42.06 PS.reconfigure :default @23:24:42.58 PS.seek 0.5ml @23:24:48.59 delay 5 seconds @23:24:51.29 Intake.select :close @23:24:51.45 SSV.dial :exhaust @23:24:52.22 Exhaust.select :open @23:24:52.34 SS.seek 0ml @23:24:53.61 PSR.select :air @23:24:53.73 PS.seek 2ml @23:25:01.38 Exhaust.select :close @23:25:01.49 SSV.dial CBV @23:25:01.54 CBV.dial SSV @23:25:02.30 Allowing 130s for 25.0ml of remaining 500.0ml @23:25:02.32 Intake.select :open @23:25:04.48 Intake @15.5psia, vacuum from 10.0 to 13.0psi @23:25:04.50 SS.reconfigure :inhaling 0.59ml/s @23:25:04.55 SS.seek 25ml @23:25:09.87 PTV.dial :puck @23:25:10.62 PSV.dial PTV @23:25:11.50 PBV.dial :waste1 @23:25:11.94 PS.seek 1.5ml @23:25:17.87 delay 3 seconds @23:25:20.89 PBV.dial between PSV and waste1 @23:25:21.27 PS.seek 0ml @23:25:37.47 delay 2:00 @23:25:48.83 Vacuum too high -- waiting up to 40 seconds @23:25:48.84 SS.reconfigure :exhale @23:25:48.90 SS.hold @23:26:07.37 Intake @17.0psia, vacuum from 10.0 to 13.0psi @23:26:07.39 SS.reconfigure :inhaling 0.47ml/s @23:26:07.44 SS.seek 25ml @23:26:07.61 Stroke completed -- waiting for pressure to equalize ... @23:26:07.62 SS.reconfigure :exhale @23:26:07.67 SS.hold @23:26:22.14 Intake.select :close @23:26:22.30 SSV.dial :exhaust @23:26:23.07 Exhaust.select :open @23:26:23.19 SS.seek 0ml @23:26:31.99 Exhaust.select :close @23:26:32.10 SSV.dial CBV @23:26:32.13 CBV.dial SSV @23:26:32.91 Allowing 130s for 25.0ml of remaining 475.0ml @23:26:32.92 Intake.select :open @23:26:35.10 Intake @15.3psia, vacuum from 9.8 to 12.8psi @23:26:35.11 SS.reconfigure :inhaling 0.47ml/s @23:26:35.16 SS.seek 25ml @23:27:30.83 Stroke completed -- waiting for pressure to equalize ... @23:27:30.84 SS.reconfigure :exhale @23:27:30.89 SS.hold @23:27:37.49 recoverPSRreagent08 @23:27:37.50 PTV.dial :puck @23:27:37.68 PBV.dial :waste1 @23:27:38.06 delay 30 seconds @23:27:57.44 Intake.select :close @23:27:57.60 SSV.dial :exhaust @23:27:58.39 Exhaust.select :open @23:27:58.51 SS.seek 0ml @23:28:07.56 Exhaust.select :close @23:28:07.67 SSV.dial CBV @23:28:07.69 CBV.dial SSV @23:28:08.08 PBV.dial between PSV and waste1 @23:28:08.45 PSR.select :air @23:28:08.47 PSV.dial PSR @23:28:08.54 Allowing 130s for 25.0ml of remaining 450.0ml @23:28:08.55 Intake.select :open @23:28:09.24 PS.reconfigure :fast @23:28:09.29 PS.seek 2.5ml @23:28:10.72 Intake @15.6psia, vacuum from 10.0 to 13.0psi @23:28:10.74 SS.reconfigure :inhaling 0.47ml/s @23:28:10.79 SS.seek 25ml @23:28:33.25 PSV.dial PTV @23:28:34.61 delay 5 seconds @23:28:39.63 PSV.dial PSR @23:28:40.39 delay 5 seconds @23:28:45.41 PSV.dial PTV @23:28:46.29 delay 5 seconds @23:28:51.32 PSV.dial :air @23:28:52.21 PS.seek 0ml @23:29:05.75 Stroke completed -- waiting for pressure to equalize ... @23:29:05.76 SS.reconfigure :exhale @23:29:05.81 SS.hold @23:29:15.62 washCycleDA #2 of 3 @23:29:15.63 PSR.select :air @23:29:15.65 PSV.dial PSR @23:29:17.12 Pulling 0.5ml of PSR.wash @23:29:17.13 PSR.select :wash @23:29:17.25 PS.reconfigure :default @23:29:17.29 PS.seek 0.5ml @23:29:23.31 delay 5 seconds @23:29:28.32 PSR.select :air @23:29:28.44 PS.seek 2ml @23:29:36.87 Intake.select :close @23:29:37.03 SSV.dial :exhaust @23:29:37.81 Exhaust.select :open @23:29:37.93 SS.seek 0ml @23:29:44.65 PTV.dial :puck @23:29:44.73 PSV.dial PTV @23:29:45.48 PBV.dial :waste1 @23:29:45.93 PS.seek 1.5ml @23:29:46.76 Exhaust.select :close @23:29:46.87 SSV.dial CBV @23:29:46.92 CBV.dial SSV @23:29:47.67 Allowing 130s for 25.0ml of remaining 425.0ml @23:29:47.68 Intake.select :open @23:29:49.86 Intake @15.5psia, vacuum from 10.0 to 13.0psi @23:29:49.88 SS.reconfigure :inhaling 0.47ml/s @23:29:49.93 SS.seek 25ml @23:29:51.87 delay 3 seconds @23:29:54.89 PBV.dial between PSV and waste1 @23:29:55.27 PS.seek 0ml @23:30:11.40 delay 2:00 @23:30:45.54 Stroke completed -- waiting for pressure to equalize ... @23:30:45.56 SS.reconfigure :exhale @23:30:45.60 SS.hold @23:31:18.46 Intake.select :close @23:31:18.62 SSV.dial :exhaust @23:31:19.41 Exhaust.select :open @23:31:19.53 SS.seek 0ml @23:31:28.61 Exhaust.select :close @23:31:28.71 SSV.dial CBV @23:31:28.76 CBV.dial SSV @23:31:29.50 Allowing 130s for 25.0ml of remaining 400.0ml @23:31:29.95 Intake.select :open @23:31:32.15 Intake @15.4psia, vacuum from 9.9 to 12.9psi @23:31:32.17 SS.reconfigure :inhaling 0.47ml/s @23:31:32.22 SS.seek 25ml @23:32:11.43 recoverPSRreagent08 @23:32:11.44 PTV.dial :puck @23:32:11.51 PBV.dial :waste1 @23:32:11.88 delay 30 seconds @23:32:27.42 Stroke completed -- waiting for pressure to equalize ... @23:32:27.43 SS.reconfigure :exhale @23:32:27.48 SS.hold @23:32:41.90 PBV.dial between PSV and waste1 @23:32:42.27 PSR.select :air @23:32:42.29 PSV.dial PSR @23:32:43.06 PS.reconfigure :fast @23:32:43.11 PS.seek 2.5ml @23:33:06.35 Intake.select :close @23:33:06.51 SSV.dial :exhaust @23:33:07.02 PSV.dial PTV @23:33:07.28 Exhaust.select :open @23:33:07.40 SS.seek 0ml @23:33:07.79 delay 5 seconds @23:33:12.81 PSV.dial PSR @23:33:13.59 delay 5 seconds @23:33:16.28 Exhaust.select :close @23:33:16.39 SSV.dial CBV @23:33:16.42 CBV.dial SSV @23:33:17.20 Allowing 130s for 25.0ml of remaining 375.0ml @23:33:17.21 Intake.select :open @23:33:18.60 PSV.dial PTV @23:33:19.38 Intake @15.5psia, vacuum from 10.0 to 13.0psi @23:33:19.40 SS.reconfigure :inhaling 0.47ml/s @23:33:19.45 SS.seek 25ml @23:33:19.52 delay 5 seconds @23:33:24.55 PSV.dial :air @23:33:25.34 PS.seek 0ml @23:33:48.68 washCycleDA #3 of 3 @23:33:48.69 PSR.select :air @23:33:48.71 PSV.dial PSR @23:33:50.05 Pulling 0.5ml of PSR.wash @23:33:50.06 PSR.select :wash @23:33:50.18 PS.reconfigure :default @23:33:50.23 PS.seek 0.5ml @23:33:56.37 delay 5 seconds @23:34:01.39 PSR.select :air @23:34:01.51 PS.seek 2ml @23:34:14.94 Vacuum too high -- waiting up to 40 seconds @23:34:14.96 SS.reconfigure :exhale @23:34:15.01 SS.hold @23:34:17.71 PTV.dial :puck @23:34:17.82 PSV.dial PTV @23:34:18.57 PBV.dial :waste1 @23:34:19.12 PS.seek 1.5ml @23:34:25.05 delay 3 seconds @23:34:28.07 PBV.dial between PSV and waste1 @23:34:28.43 PS.seek 0ml @23:34:38.57 Intake @16.6psia, vacuum from 10.0 to 13.0psi @23:34:38.59 SS.reconfigure :inhaling 0.38ml/s @23:34:38.64 SS.seek 25ml @23:34:38.80 Stroke completed -- waiting for pressure to equalize ... @23:34:38.82 SS.reconfigure :exhale @23:34:38.87 SS.hold @23:34:44.58 delay 2:00 @23:34:55.25 Intake.select :close @23:34:55.41 SSV.dial :exhaust @23:34:56.20 Exhaust.select :open @23:34:56.32 SS.seek 0ml @23:35:05.15 Exhaust.select :close @23:35:05.27 SSV.dial CBV @23:35:05.78 CBV.dial SSV @23:35:06.54 Allowing 130s for 25.0ml of remaining 350.0ml @23:35:06.55 Intake.select :open @23:35:08.73 Intake @15.6psia, vacuum from 10.0 to 13.0psi @23:35:08.75 SS.reconfigure :inhaling 0.38ml/s @23:35:08.80 SS.seek 25ml @23:36:17.16 Stroke completed -- waiting for pressure to equalize ... @23:36:17.17 SS.reconfigure :exhale @23:36:17.22 SS.hold @23:36:44.60 recoverPSRreagent08 @23:36:44.62 PTV.dial :puck @23:36:44.69 PBV.dial :waste1 @23:36:45.05 delay 30 seconds @23:36:54.02 Intake.select :close @23:36:54.18 SSV.dial :exhaust @23:36:54.96 Exhaust.select :open @23:36:55.08 SS.seek 0ml @23:37:03.90 Exhaust.select :close @23:37:04.01 SSV.dial CBV @23:37:04.03 CBV.dial SSV @23:37:04.82 Allowing 130s for 25.0ml of remaining 325.0ml @23:37:04.83 Intake.select :open @23:37:07.00 Intake @15.5psia, vacuum from 10.0 to 13.0psi @23:37:07.02 SS.reconfigure :inhaling 0.38ml/s @23:37:07.08 SS.seek 25ml @23:37:15.07 PBV.dial between PSV and waste1 @23:37:15.44 PSR.select :air @23:37:15.46 PSV.dial PSR @23:37:16.23 PS.reconfigure :fast @23:37:16.28 PS.seek 2.5ml @23:37:40.17 PSV.dial PTV @23:37:40.94 delay 5 seconds @23:37:45.96 PSV.dial PSR @23:37:46.72 delay 5 seconds @23:37:51.74 PSV.dial PTV @23:37:52.53 delay 5 seconds @23:37:57.56 PSV.dial :air @23:37:58.43 PS.seek 0ml @23:38:15.92 Stroke completed -- waiting for pressure to equalize ... @23:38:15.94 SS.reconfigure :exhale @23:38:15.99 SS.hold @23:38:21.48 Gate.power :camera,:ON @23:38:21.50 Mixing DA Substrates @23:38:21.51 PSR.select :air @23:38:21.53 PSV.dial PSR @23:38:23.10 Pulling 0.25ml of PSR.sub1 @23:38:23.16 PSR.select :sub1 @23:38:23.35 PS.reconfigure :default @23:38:23.47 PS.seek 0.25ml @23:38:27.08 delay 5 seconds @23:38:32.10 Pulling 0.25ml of PSR.sub2 @23:38:32.11 PSR.select :sub2 @23:38:32.22 PS.seek 0.5ml @23:38:35.58 delay 5 seconds @23:38:40.60 PSR.select :air @23:38:40.71 PS.seek 2ml @23:38:56.17 Intake.select :close @23:38:56.33 SSV.dial :exhaust @23:38:56.92 PTV.dial :puck @23:38:57.00 PSV.dial PTV @23:38:57.11 Exhaust.select :open @23:38:57.23 SS.seek 0ml @23:38:57.80 PBV.dial :waste1 @23:38:58.24 PS.seek 1.5ml @23:39:04.17 delay 3 seconds @23:39:06.36 Exhaust.select :close @23:39:06.47 SSV.dial CBV @23:39:06.50 CBV.dial SSV @23:39:07.19 PBV.dial between PSV and waste1 @23:39:07.30 Allowing 130s for 25.0ml of remaining 300.0ml @23:39:07.32 Intake.select :open @23:39:07.57 PS.seek 0ml @23:39:09.49 Intake @15.6psia, vacuum from 10.0 to 13.0psi @23:39:09.51 SS.reconfigure :inhaling 0.38ml/s @23:39:09.56 SS.seek 25ml @23:39:23.77 delay 10 seconds @23:39:33.80 recoverPSRreagent08 PTV.dial :puck @23:39:33.87 PBV.dial :waste1 @23:39:34.27 delay 30 seconds @23:40:04.29 PBV.dial between PSV and waste1 @23:40:04.67 PSR.select :air @23:40:04.69 PSV.dial PSR @23:40:05.47 PS.reconfigure :fast @23:40:05.53 PS.seek 2.5ml @23:40:17.43 Vacuum too high -- waiting up to 40 seconds @23:40:17.45 SS.reconfigure :exhale @23:40:17.51 SS.hold @23:40:29.64 PSV.dial PTV @23:40:30.42 delay 5 seconds @23:40:35.44 PSV.dial PSR @23:40:36.29 delay 5 seconds @23:40:41.30 PSV.dial PTV @23:40:42.08 delay 5 seconds @23:40:42.46 Intake @16.5psia, vacuum from 10.0 to 13.0psi @23:40:42.48 SS.reconfigure :inhaling 0.31ml/s @23:40:42.53 SS.seek 25ml @23:40:43.42 Stroke completed -- waiting for pressure to equalize ... @23:40:43.43 SS.reconfigure :exhale @23:40:43.48 SS.hold @23:40:47.09 PSV.dial :air @23:40:47.88 PS.seek 0ml @23:41:06.45 Intake.select :close @23:41:06.61 SSV.dial :exhaust @23:41:07.40 Exhaust.select :open @23:41:07.52 SS.seek 0ml @23:41:11.58 Additional air clear @23:41:11.59 PSR.select :air @23:41:11.61 PSV.dial PSR @23:41:12.98 Pulling 2ml of PSR.air @23:41:12.99 PSR.select :air @23:41:13.02 PS.reconfigure :default @23:41:13.06 PS.seek 2ml @23:41:16.69 Exhaust.select :close @23:41:17.29 SSV.dial CBV @23:41:17.31 CBV.dial SSV @23:41:18.15 Allowing 130s for 25.0ml of remaining 275.0ml @23:41:18.17 Intake.select :open @23:41:20.33 Intake @15.6psia, vacuum from 10.0 to 13.0psi @23:41:20.35 SS.reconfigure :inhaling 0.31ml/s @23:41:20.40 SS.seek 25ml @23:41:34.39 delay 5 seconds @23:41:39.41 PTV.dial :puck @23:41:39.50 PSV.dial PTV @23:41:40.28 PBV.dial :waste1 @23:41:40.72 PS.seek 1.5ml @23:41:46.73 delay 3 seconds @23:41:49.75 PBV.dial between PSV and waste1 @23:41:50.12 delay 1 second @23:41:51.14 PSV.dial :waste1 @23:41:52.22 PS.seek 0ml @23:42:08.44 Processing Puck Heater at 28C before imaging @23:42:08.45 PH.coast @23:42:08.55 Imaging da puck in Processing Clamp while storing it to tube 4 @23:42:08.73 SC.reconfigure :tight @23:42:08.78 SC.seek 4 @23:42:12.37 SC.configure :limp @23:42:12.59 Elbow.reconfigure :tight @23:42:12.64 Elbow.seek 4 @23:42:14.41 Forearm.reconfigure :tight @23:42:14.47 Forearm.seek 4 + 450 counts @23:42:15.55 SE.reconfigure :pick @23:42:15.61 SE.seek :beamBreak @23:42:18.17 SE.configure :default @23:42:18.34 Elbow.reconfigure :limp @23:42:18.39 Forearm.reconfigure :loose @23:42:18.44 Forearm.seek 4 @23:42:18.72 Elbow.hold @23:42:18.79 Elbow.reconfigure :tight @23:42:18.89 SE.seek 10.14 Pucks below Top Plate @23:42:19.35 Forearm.force -20 @23:42:19.40 Forearm.seek 4 + 296 counts @23:42:20.17 Forearm.reconfigure :tight @23:42:20.22 Forearm.seek 4 + 450 counts @23:42:20.52 SE.seek 11.14 Pucks below Top Plate @23:42:21.03 Forearm.reconfigure :loose @23:42:21.08 Forearm.seek :retract @23:42:22.02 Elbow.seek PC @23:42:24.57 Elbow.reconfigure :loose @23:42:24.67 Forearm.seek PC @23:42:25.62 Hand.close @23:42:26.25 PC.seek :open @23:42:31.06 Forearm.seek :retract @23:42:32.03 Elbow.reconfigure :tight @23:42:32.08 Elbow.seek 4 @23:42:34.70 Forearm.reconfigure :tight @23:42:34.75 Forearm.seek 4 + 450 counts @23:42:35.67 Hand.open @23:42:36.32 SE.seek 10.79 Pucks below Top Plate @23:42:36.61 SE.reconfigure :seal @23:42:36.66 SE.seek 9.83 Pucks below Top Plate @23:42:37.23 Lights, camera, ACTION! @23:42:37.27 PuckCamera: snap -bin=2,2 -auto /var/log/esp/da23dec0423h1000ml.tif @23:42:47.55 Stroke completed -- waiting for pressure to equalize ... @23:42:47.56 SS.reconfigure :exhale @23:42:47.62 SS.hold @23:42:56.71 Waiting >20 seconds for da to relinquish FlushPuck @23:42:58.54 SX HX9/16 (USB:15): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 17.977 seconds /var/log/esp/da23dec0423h1000ml.tif: TIFF Upload Complete @23:42:58.57 PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da23dec0423h1000ml15s.tif @23:43:14.18 SX HX9/16 (USB:15): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15 seconds /var/log/esp/midres/da23dec0423h1000ml15s.tif: TIFF Upload Complete @23:43:14.21 PuckCamera: snap -bin=2,2 20.0 /var/log/esp/midres/da23dec0423h1000ml20s.tif @23:43:24.69 Intake.select :close @23:43:24.86 SSV.dial :exhaust @23:43:25.64 Exhaust.select :open @23:43:25.76 SS.seek 0ml @23:43:34.77 Exhaust.select :close @23:43:34.95 SSV.dial CBV @23:43:35.00 CBV.dial SSV @23:43:35.05 SX HX9/16 (USB:15): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 20 seconds /var/log/esp/midres/da23dec0423h1000ml20s.tif: TIFF Upload Complete @23:43:35.08 Gate.power :camera,:OFF @23:43:35.86 DAfans.email "17.977 sec auto-exposure",Subject: "da23dec0423h1000ml images!" @23:43:35.97 Allowing 130s for 25.0ml of remaining 250.0ml @23:43:36.00 Intake.select :open @23:43:36.22 Elbow.seek 4 @23:43:36.44 Forearm.seek 4 + 450 counts @23:43:36.53 SE.reconfigure :gently @23:43:36.59 SE.seek 10.54 Pucks below Top Plate @23:43:37.24 Elbow.reconfigure :limp @23:43:37.30 Forearm.reconfigure :loose @23:43:37.35 Forearm.seek 4 @23:43:37.61 Elbow.hold @23:43:37.69 Elbow.reconfigure :tight @23:43:37.75 SE.reconfigure :default @23:43:37.80 SE.seek 10.73 Pucks below Top Plate @23:43:38.18 Forearm.force -20 @23:43:38.23 Forearm.seek 4 + 315 counts @23:43:38.38 Intake @15.6psia, vacuum from 10.0 to 13.0psi @23:43:38.40 SS.reconfigure :inhaling 0.31ml/s @23:43:38.47 SS.seek 25ml @23:43:38.77 Forearm.reconfigure :tight @23:43:38.82 Forearm.seek 4 + 450 counts @23:43:39.10 SE.seek :down @23:43:40.33 Forearm.reconfigure :loose @23:43:40.44 Forearm.seek :retract @23:43:41.19 SE.stop @23:43:41.66 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @23:43:42.07 Elbow.seek FlushPuck::Garage @23:43:44.15 Elbow.reconfigure :loose @23:43:45.32 Forearm.seek :garage @23:43:46.45 Hand.close @23:43:47.42 Forearm.seek :retract @23:43:48.43 Elbow.reconfigure :tight @23:43:48.54 Elbow.seek PC @23:43:49.90 Elbow.reconfigure :loose @23:43:50.12 Forearm.reconfigure :tight @23:43:50.22 Forearm.seek PC @23:43:51.86 PC.seek :closed @23:43:56.79 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.66 @23:43:56.93 Hand.open @23:43:57.78 Forearm.reconfigure :loose @23:43:57.87 Forearm.seek :retract @23:43:58.84 PSR.select :air @23:43:58.89 PSV.dial PSR @23:43:59.40 Pulling 0.5ml of PSR.flush @23:43:59.42 PSR.select :flush @23:43:59.61 PS.seek 0.5ml @23:44:05.62 delay 5 seconds @23:44:09.38 upsync @23:44:10.65 PSR.select :air @23:44:10.77 PS.seek 2.5ml @23:44:32.13 delay 1 second @23:44:33.15 PRV.dial :waste2,avoiding: :ab2 @23:44:35.06 PTV.dial :PRVmixing @23:44:36.38 PSV.dial :mixloop @23:44:37.44 PS.seek :empty @23:45:03.85 PSR.select :air @23:45:03.87 PSV.dial PSR @23:45:04.93 Pulling 0.5ml of PSR.flush @23:45:04.94 PSR.select :flush @23:45:05.06 PS.seek 0.5ml @23:45:06.17 Stroke completed -- waiting for pressure to equalize ... @23:45:06.18 SS.reconfigure :exhale @23:45:06.23 SS.hold @23:45:10.98 delay 5 seconds @23:45:15.99 PSR.select :air @23:45:16.11 PS.seek 2.5ml @23:45:37.38 delay 1 second @23:45:38.78 PBV.dial :waste1 @23:45:39.23 PTV.dial :mixing @23:45:39.99 PSV.dial :mixloop @23:45:41.08 PS.seek :empty @23:45:46.01 SS.reconfigure :default @23:45:46.06 SS.hold @23:45:46.13 Intake.select :close @23:45:46.29 ShallowSampler::Clogged in WCR -- Filtering too Slowly after 775.0ml @23:45:46.30 SSV.dial :exhaust @23:45:47.07 Exhaust.select :open @23:45:47.19 SS.reconfigure :exhale @23:45:47.24 SS.seek 0ml @23:45:56.06 Exhaust.select :close @23:45:56.17 Sampled 775.0ml @23:45:56.19 Drying puck before fixing for 5:00 @23:45:56.20 CBV.dial :waste2b @23:45:56.97 delay 1:00 @23:46:07.41 PSV.dial PSR @23:46:08.48 PSR.select :flush @23:46:08.60 PS.seek 1ml @23:46:19.69 PSR.select :air @23:46:19.81 PS.seek 2.5ml @23:46:36.16 PSV.dial PTV @23:46:37.05 PTV.dial :puck @23:46:37.80 PBV.dial :waste1 @23:46:37.89 PS.seek 0ml @23:46:57.00 CSV.dial :air @23:46:57.46 CS.reconfigure :fast @23:46:57.51 CS.seek 10ml @23:47:04.22 PSV.dial PSR @23:47:04.98 PSR.select :flush @23:47:05.09 PS.seek 0.5ml @23:47:11.19 PSR.select :air @23:47:11.31 PS.seek 2.5ml @23:47:22.05 Pumping wcr puck from tube 5 up to 35psi... @23:47:22.07 CBV.dial SSV @23:47:23.35 CBV.dial between CSV and waste2b @23:47:24.32 CSV.dial between air and CTV @23:47:25.09 CS.seek 7.286ml @23:47:32.21 CTV.dial CSV,avoiding: :air @23:47:32.23 CSV.dial CTV @23:47:32.60 PSV.dial PTV @23:47:33.07 CS.reconfigure :pressurize @23:47:33.12 CS.seek 0ml @23:47:33.37 PBV.dial PSV @23:47:33.93 PS.seek 0ml @23:47:51.50 CBV.dial :waste2b @23:47:51.83 CS.reconfigure :default @23:47:51.88 CS.seek 0ml @23:47:53.33 delay 1:00 @23:48:00.22 PSV.dial PBV @23:48:00.70 PS.seek 2.5ml @23:48:27.07 PSV.dial :waste1 @23:48:27.82 PS.seek 0ml @23:48:53.35 CTV.dial :air,avoiding: :intake @23:48:54.19 PSV.dial PSR @23:48:54.66 PSR.select :air @23:48:54.69 PS.seek 2.5ml @23:48:54.76 Fix #1 of 2 (with RNAlater) for 2:30 @23:48:54.78 CSV.dial CSR @23:48:55.34 Pulling 2ml of CSR.RNAlater @23:48:55.35 CSR.select :RNAlater @23:48:55.47 CS.seek 2ml @23:49:01.36 delay 5 seconds @23:49:06.38 CSR.select :air @23:49:06.50 CS.reconfigure :fast @23:49:06.55 CS.seek 10ml @23:49:21.10 PSV.dial PTV @23:49:21.85 PBV.dial PSV @23:49:21.93 PS.seek 0ml @23:49:26.34 Pumping wcr puck from tube 5 up to 35psi... @23:49:26.35 CBV.dial SSV @23:49:27.63 CBV.dial between CSV and waste2b @23:49:28.61 CSV.dial between air and CTV @23:49:29.19 CS.seek 7.106ml @23:49:36.65 CTV.dial CSV,avoiding: :air @23:49:36.67 CSV.dial CTV @23:49:38.52 CS.reconfigure :pressurize @23:49:38.57 CS.seek 0ml @23:49:48.28 PSV.dial PBV @23:49:48.78 PS.seek 2.5ml @23:49:53.91 delay 2:30 @23:50:15.16 PSV.dial :waste1 @23:50:15.90 PS.seek 0ml @23:50:42.28 PSV.dial PSR @23:50:42.75 PSR.select :flush @23:50:42.88 PS.seek 1ml @23:50:53.94 PSR.select :air @23:50:54.06 PS.seek 2.5ml @23:51:10.28 PSV.dial PTV @23:51:11.03 PTV.dial :puck @23:51:11.05 PBV.dial :waste1 @23:51:11.62 PS.seek 0ml @23:51:37.97 PSV.dial PSR @23:51:38.86 PSR.select :flush @23:51:38.98 PS.seek 0.5ml @23:51:44.97 PSR.select :air @23:51:45.09 PS.seek 2.5ml @23:52:06.28 PSV.dial PTV @23:52:07.03 PBV.dial PSV @23:52:07.60 PS.seek 0ml @23:52:23.93 CBV.dial :waste2b @23:52:24.28 CS.reconfigure :default @23:52:24.34 CS.seek 0ml @23:52:28.32 delay 1:00 @23:52:33.97 PSV.dial PBV @23:52:34.46 PS.seek 2.5ml @23:53:00.78 PSV.dial :waste1 @23:53:01.52 PS.seek 0ml @23:53:27.81 PSV.dial PSR @23:53:28.28 PSR.select :air @23:53:28.31 PS.seek 2.5ml @23:53:28.36 CTV.dial :air,avoiding: :intake @23:53:29.70 Fix #2 of 2 (with RNAlater) for 2:30 @23:53:29.72 CSV.dial CSR @23:53:30.17 Pulling 2ml of CSR.RNAlater @23:53:30.18 CSR.select :RNAlater @23:53:30.30 CS.seek 2ml @23:53:36.20 delay 5 seconds @23:53:41.22 CSR.select :air @23:53:41.34 CS.reconfigure :fast @23:53:41.39 CS.seek 10ml @23:53:54.84 PSV.dial PTV @23:53:55.59 PBV.dial PSV @23:53:55.79 PS.seek 0ml @23:54:01.48 Pumping wcr puck from tube 5 up to 35psi... @23:54:01.50 CBV.dial SSV @23:54:02.77 CBV.dial between CSV and waste2b @23:54:03.76 CSV.dial between air and CTV @23:54:04.36 CS.seek 7.138ml @23:54:11.67 CTV.dial CSV,avoiding: :air @23:54:11.70 CSV.dial CTV @23:54:13.08 CS.reconfigure :pressurize @23:54:13.13 CS.seek 0ml @23:54:22.15 PSV.dial PBV @23:54:22.65 PS.seek 2.5ml @23:54:26.81 delay 2:30 @23:54:48.93 PSV.dial :waste1 @23:54:49.68 PS.seek 0ml @23:55:16.09 Garaging FlushPuck in Processing Clamp @23:55:16.16 Elbow.reconfigure :tight @23:55:16.21 Elbow.seek PC @23:55:16.33 Elbow.reconfigure :loose @23:55:16.43 Forearm.seek PC @23:55:17.39 Hand.close @23:55:17.99 PC.seek :open @23:55:22.71 Forearm.seek :retract @23:55:23.68 Elbow.reconfigure :tight @23:55:23.73 Elbow.seek :garage @23:55:25.09 Elbow.reconfigure :loose @23:55:25.19 Forearm.reconfigure :tight @23:55:25.24 Forearm.seek :garage @23:55:26.18 Hand.open @23:55:26.80 Forearm.reconfigure :loose @23:55:26.85 Forearm.seek :retract @23:56:56.84 CBV.dial :waste2b @23:56:57.18 CS.reconfigure :default @23:56:57.68 CS.seek 0ml @23:57:03.45 delay 1:00 @23:58:03.47 CTV.dial :air,avoiding: :intake @23:58:04.85 Storing wcr puck in Collection Clamp to tube 4 @23:58:05.04 SC.reconfigure :tight @23:58:05.09 SC.seek 4 @23:58:05.15 SC.configure :limp @23:58:05.37 Elbow.reconfigure :tight @23:58:05.42 Elbow.seek 4 @23:58:07.28 Forearm.reconfigure :tight @23:58:07.33 Forearm.seek 4 + 450 counts @23:58:08.51 SE.reconfigure :pick @23:58:08.57 SE.seek :beamBreak @23:58:10.99 SE.configure :default @23:58:11.16 Elbow.reconfigure :limp @23:58:11.21 Forearm.reconfigure :loose @23:58:11.26 Forearm.seek 4 @23:58:11.49 Elbow.hold @23:58:11.56 Elbow.reconfigure :tight @23:58:11.66 SE.seek 11.13 Pucks below Top Plate @23:58:12.11 Forearm.force -20 @23:58:12.16 Forearm.seek 4 + 317 counts @23:58:12.45 Forearm.reconfigure :tight @23:58:12.51 Forearm.seek 4 + 450 counts @23:58:12.70 SE.seek 12.13 Pucks below Top Plate @23:58:13.22 Forearm.reconfigure :loose @23:58:13.72 Forearm.seek :retract @23:58:14.65 Elbow.seek CC @23:58:16.30 Elbow.reconfigure :loose @23:58:16.40 Forearm.seek CC @23:58:17.41 Hand.close @23:58:17.99 CC.seek :open @23:58:22.74 Forearm.seek :retract @23:58:23.73 Elbow.reconfigure :tight @23:58:23.78 Elbow.seek 4 @23:58:25.56 Elbow.reconfigure :limp @23:58:25.61 Forearm.seek 4 @23:58:26.66 Elbow.hold @23:58:26.73 Elbow.reconfigure :tight @23:58:26.79 Hand.open @23:58:27.49 Forearm.force -20 @23:58:27.54 Forearm.seek 4 + 319 counts @23:58:27.83 Forearm.reconfigure :tight @23:58:27.88 Forearm.seek 4 + 450 counts @23:58:28.14 SE.seek :down @23:58:29.28 Forearm.reconfigure :loose @23:58:29.33 Forearm.seek :retract @23:58:30.02 SE.stop @23:58:30.30
Starting finalPurge @23:58:30.34 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @23:58:30.55 Elbow.seek FlushPuck::Garage @23:58:32.80 Elbow.reconfigure :loose @23:58:32.90 Forearm.seek :garage @23:58:33.88 Hand.close @23:58:34.61 Forearm.seek :retract @23:58:35.60 Elbow.reconfigure :tight @23:58:35.65 Elbow.seek CC @23:58:38.60 Elbow.reconfigure :loose @23:58:38.70 Forearm.reconfigure :tight @23:58:38.75 Forearm.seek CC @23:58:40.11 CC.seek :closed @23:58:44.64 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.36 @23:58:44.70 Hand.open @23:58:45.42 Forearm.reconfigure :loose @23:58:45.47 Forearm.seek :retract @23:58:46.46 Flushing the Collection Valve::Manifold @23:58:46.59 CSV.dial CSR @23:58:47.02 Pulling 5ml of CSR.flush @23:58:47.03 CSR.select :flush @23:58:47.15 CS.seek 5ml @23:59:00.75 delay 5 seconds @23:59:05.77 CSR.select :air @23:59:05.94 CS.reconfigure :fast @23:59:05.99 CS.seek 7.501ml @23:59:12.72 CSV.dial :waste @23:59:14.02 CS.reconfigure :default @23:59:14.07 CS.seek 0ml @23:59:34.13 Flushing lower manifold's waste1 port @23:59:34.25 CSV.dial :flush @23:59:34.71 CS.reconfigure :fast @23:59:34.76 CS.seek 5ml @23:59:47.66 delay 5 seconds @23:59:52.68 CSV.dial :air @23:59:53.19 CS.seek 7ml @23:59:58.79 CSV.dial CTV @23:59:58.81
CTV.dial CSV @00:00:00.19UTC05-Dec-23 CBV.dial :waste1 @00:00:00.96 CS.seek 0ml @00:00:17.66 Flushing lower manifold's waste2 port @00:00:17.79 CSV.dial :flush @00:00:18.53 CS.seek 5ml @00:00:31.25 delay 5 seconds @00:00:36.27 CSV.dial :air @00:00:36.80 CS.seek 7.001ml @00:00:42.41 CSV.dial CTV @00:00:42.43
CTV.dial CSV @00:00:42.91 CBV.dial :waste2 @00:00:43.96 CS.seek 0ml @00:01:00.66 Flushing lower manifold's waste2b port @00:01:00.78 CSV.dial :flush @00:01:01.53 CS.seek 5ml @00:01:14.19 delay 5 seconds @00:01:19.21 CSV.dial :air @00:01:19.74 CS.seek 7ml @00:01:25.35 CSV.dial CTV @00:01:25.37
CTV.dial CSV @00:01:25.83 CBV.dial :waste2b @00:01:26.88 CS.seek 0ml @00:01:43.69 Disinfecting external Intake with kill @00:01:43.81 CSV.dial CSR @00:01:44.25 Pulling 8ml of CSR.kill @00:01:44.26 CSR.select :kill @00:01:44.38 CS.seek 8ml @00:02:04.16 delay 5 seconds @00:02:09.18 CSR.select :air @00:02:09.30 CS.seek 10ml @00:02:14.91 CSV.dial CBV @00:02:14.93
CBV.dial CSV @00:02:16.13 CTV.dial :intake @00:02:16.91 Intake.select :open @00:02:17.02 CS.seek 2ml @00:02:36.09 Intake.select :close @00:02:36.20 CTV.dial :air @00:02:36.97 CS.reconfigure :default @00:02:37.02 CS.seek 0ml @00:02:43.06 CSV.dial CSR @00:02:44.06 Pulling 5ml of CSR.flush @00:02:44.07 CSR.select :flush @00:02:44.58 CS.seek 5ml @00:02:58.38 delay 5 seconds @00:03:03.39 CSR.select :air @00:03:03.51 CS.seek 10ml @00:03:17.09 CSV.dial CBV @00:03:18.12 CS.seek 0ml @00:03:44.56 Garaging FlushPuck in Collection Clamp @00:03:44.63 Elbow.reconfigure :tight @00:03:44.68 Elbow.seek CC @00:03:44.96 Elbow.reconfigure :loose @00:03:45.06 Forearm.seek CC @00:03:46.08 Hand.close @00:03:46.74 CC.seek :open @00:03:51.37 Forearm.seek :retract @00:03:52.33 Elbow.reconfigure :tight @00:03:52.38 Elbow.seek :garage @00:03:55.35 Elbow.reconfigure :loose @00:03:55.46 Forearm.reconfigure :tight @00:03:55.50 Forearm.seek :garage @00:03:56.50 Hand.open @00:03:57.17 Forearm.reconfigure :loose @00:03:57.22 Forearm.seek :retract @00:03:58.22 Completed [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] begun 21:15:20 @00:03:58.27 Asleep after completing DA_WCR_120423b assays @00:03:58.29 GoodNews.email "{1 => 33,\n 2 => 33,\n 3 => 31,\n 4 => 13,\n 5 => 20,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@23:54:40, 31% flow, 16.4C, 29% humidity, 15.1psia, 13.695V, 0.478A, 149.440Ah, 6.54W\nSampled 775.0ml",Subject: "Asleep after completing DA_WCR_120423b assays" @00:04:02.71 upsync + ESPwhich DA_WCR_120523.rb + fn=DA_WCR_120523.rb + IFS=: + set /home/esp/esp2/mission . + fullPath=/home/esp/esp2/mission/DA_WCR_120523.rb + [ -f /home/esp/esp2/mission/DA_WCR_120523.rb ] + realpath /home/esp/esp2/mission/DA_WCR_120523.rb + return 0 + scriptPath=/home/esp/esp2local/mission/DA_WCR_120523.rb + exec ruby /home/esp/esp2/lib/esp.rb /home/esp/esp2local/mission/DA_WCR_120523.rb @12:42:46.68UTC05-Dec-23
ESP2 v3.42 I2C Gateway v3.20 for core bus @12:42:49.37 Configuration for short puck jake 1/11/21 by brent@mbari.org /home/esp/esp2/type/shallow/jake/configure.rb @12:42:52.97 Polling Can every 10:00 @12:42:53.01 Cleanly exited at 12:42:26UTC05-Dec-23 @12:42:53.06 Loading /home/esp/esp2local/mission/DA_WCR_120523.rb mission @12:42:53.11 Preparing to sleep until + 00:00:05 @12:42:53.13 GoodNews.email "Starting DA_WCR_120523 w/pucks in tube 5\n{1 => 33,\n 2 => 33,\n 3 => 31,\n 4 => 13,\n 5 => 20,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@12:42:52, 31% flow, 16.1C, 30% humidity, 15.1psia, 13.739V, 0.296A, 153.077Ah, 4.07W",Subject: "DA_WCR_120523 begun" @12:42:53.18
Asleep til + 00:00:05 @12:42:53.22 Continuing at 12:42 @12:42:53.45 Gate.power :core,:OFF @12:42:53.47 Gate.power :raw,:OFF @12:42:53.48 Shutdown @12:42:56.64 GoodNews.email "Then, in tube 5: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_120523.rb:14\n{1 => 33,\n 2 => 33,\n 3 => 31,\n 4 => 13,\n 5 => 20,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@12:42:53, 31% flow, 16.1C, 30% humidity, 15.1psia, 13.739V, 0.302A, 153.077Ah, 4.16W",Subject: "Asleep until 12:42" @12:42:57.96 upsync @12:42:58.10
Powering core bus @12:43:01.27 upsync @12:43:03.83
Gate.power :raw,:ON @12:43:04.85 CS.configure :default @12:43:04.89 PS.configure :default @12:43:04.94 SS.configure :default @12:43:04.99 CC.configure :default @12:43:05.04 PC.configure :default @12:43:05.11 Forearm.configure :loose @12:43:05.16 Elbow.configure :loose @12:43:05.23 SC.configure :loose @12:43:05.28 SE.configure :default @12:43:05.33 CH.configure :default @12:43:05.36 PH.configure :default @12:43:05.40 All dwarf controllers running firmware version 3.85 @12:43:05.51 Valve.reset @12:43:05.84 Intake.select :close @12:43:05.88 PSV.dial :air @12:43:05.92 Exhaust.select :close @12:43:05.96 CTV.dial :air @12:43:06.05
Forearm.home.seek :retract @12:43:06.11 CSV.dial :waste1 @12:43:06.68 SSV.dial :spit,avoiding: :exhaust @12:43:06.86 Hand.close @12:43:06.88
Elbow.reconfigure :loose @12:43:06.99 Elbow.jog 1500 @12:43:07.12 PS.home.seek 0ml @12:43:07.50
Elbow.reconfigure :loose @12:43:07.58 Elbow.home.seek :clear @12:43:07.65 CS.reconfigure :fast @12:43:07.69 SS.home.seek 0ml @12:43:07.77 CS.home.seek 0ml @12:43:10.90
SE.home.seek :down @12:43:11.51 SC.reconfigure :tight @12:43:11.56 SC.home.seek :initial @12:43:15.32 PS.stop @12:43:30.46
SC.configure :limp @12:43:30.51 Forearm.seek :retract @12:43:30.58 Elbow.seek :hammer @12:43:31.76 Forearm.reconfigure :tight @12:43:31.82 Forearm.seek retracted @12:43:32.23 PC.home.seek :open @12:43:32.24
CC.home.seek :open @12:43:35.98 Phase [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] @12:43:36.00 Notice.email "At 12:43:35 began in tube 5: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_120523.rb:14\n{1 => 33,\n 2 => 33,\n 3 => 31,\n 4 => 13,\n 5 => 20,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@12:43:35, 31% flow, 16.1C, 30% humidity, 15.1psia, 13.749V, 0.272A, 153.081Ah, 3.74W",Subject: "Started DA_WCR_120523 phase initialPurge; da [1000, 1000] #da and wsr are run simultanelously" @12:43:36.02
Starting initialPurge @12:43:36.04 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @12:43:36.11 Hand.open @12:43:36.81 Elbow.reconfigure :tight @12:43:36.86 Elbow.seek FlushPuck::Garage @12:43:38.74 Elbow.reconfigure :loose @12:43:38.84 Forearm.reconfigure :loose @12:43:38.89 Forearm.seek :garage @12:43:39.84 Hand.close @12:43:40.43 upsync @12:43:40.59
Forearm.seek :retract @12:43:41.53 Elbow.reconfigure :tight @12:43:41.58 Elbow.seek CC @12:43:44.71 Elbow.reconfigure :loose @12:43:44.81 Forearm.reconfigure :tight @12:43:44.86 Forearm.seek CC @12:43:46.09 CC.seek :closed @12:43:50.71 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.42 @12:43:50.78 Hand.open @12:43:51.48 Forearm.reconfigure :loose @12:43:51.53 Forearm.seek :retract @12:43:52.53 Flushing the Collection Valve::Manifold @12:43:52.66 CSV.dial CSR @12:43:53.93 Pulling 5ml of CSR.flush @12:43:53.94 CSR.select :flush @12:43:54.05 CS.reconfigure :default @12:43:54.10 CS.seek 5ml @12:44:08.37 delay 5 seconds @12:44:13.39 CSR.select :air @12:44:13.56 CS.reconfigure :fast @12:44:13.60 CS.seek 7.5ml @12:44:20.46 CSV.dial :waste @12:44:21.76 CS.reconfigure :default @12:44:21.81 CS.seek 0ml @12:44:41.77 Purging external Intake @12:44:41.90 CSV.dial :flush @12:44:42.36 CS.reconfigure :fast @12:44:42.41 CS.seek 10ml @12:45:06.84 delay 5 seconds @12:45:11.86 CSV.dial CBV @12:45:11.90
CBV.dial CSV @12:45:12.63 CTV.dial :intake @12:45:13.43 Intake.select :open @12:45:13.54 CS.seek 0ml @12:45:37.21 Intake.select :close @12:45:37.31 CTV.dial :air @12:45:38.10 Flushing upper manifold @12:45:38.22 CSV.dial :flush @12:45:39.09 CS.seek 5ml @12:45:51.93 delay 5 seconds @12:45:56.95 CSV.dial :air @12:45:57.47 CS.seek 7ml @12:46:02.99 CSV.dial CBV @12:46:03.02
CBV.dial CSV @12:46:04.05 CTV.dial :air @12:46:04.14 CS.seek 0ml @12:46:20.83 delay 7 seconds @12:46:27.86 CS.seek 3ml @12:46:35.96 CSV.dial :waste @12:46:36.42 CS.seek 0ml @12:46:44.29 CSV.dial :flush @12:46:44.75 CS.seek 3ml @12:46:52.80 delay 5 seconds @12:46:57.82 CSV.dial :air @12:46:58.32 CS.seek 5ml @12:47:04.05 CSV.dial :air @12:47:04.14 CS.seek 0ml @12:47:16.27 delay 7 seconds @12:47:23.30 CS.seek 3ml @12:47:31.36 CSV.dial :waste @12:47:32.10 CS.seek 0ml @12:47:39.77 Garaging FlushPuck in Collection Clamp @12:47:39.84 Elbow.reconfigure :tight @12:47:39.89 Elbow.seek CC @12:47:40.28 Elbow.reconfigure :loose @12:47:40.38 Forearm.seek CC @12:47:41.40 Hand.close @12:47:42.04 CC.seek :open @12:47:47.10 Forearm.seek :retract @12:47:48.09 Elbow.reconfigure :tight @12:47:48.14 Elbow.seek :garage @12:47:51.10 Elbow.reconfigure :loose @12:47:51.20 Forearm.reconfigure :tight @12:47:51.25 Forearm.seek :garage @12:47:52.32 Hand.open @12:47:52.97 Forearm.reconfigure :loose @12:47:53.02 Forearm.seek :retract @12:47:54.04 da sampling at most 1000ml, wcr at most 1000ml @12:47:54.08 DAfans.email "Started da in tube 5 at 12:47:54UTC05-Dec-23\n{1 => 33,\n 2 => 33,\n 3 => 31,\n 4 => 13,\n 5 => 20,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@12:43:35, 31% flow, 16.1C, 30% humidity, 15.1psia, 13.749V, 0.272A, 153.081Ah, 3.74W",Subject: "da sampling at most 1000ml, wcr at most 1000ml" @12:47:54.10
Loading sh1 puck from tube 5 into Collection Clamp @12:47:54.36 SC.reconfigure :tight @12:47:54.42 SC.seek 5 @12:47:57.97 SC.configure :limp @12:47:58.22 Elbow.reconfigure :tight @12:47:58.27 Elbow.seek 5 @12:47:58.83 upsync @12:48:00.25
Forearm.reconfigure :tight @12:48:00.30 Forearm.seek 5 + 450 counts @12:48:01.40 SE.reconfigure :pick @12:48:01.46 SE.seek :beamBreak @12:48:03.39 SE.configure :default @12:48:03.56 Elbow.seek 5 @12:48:03.75 Forearm.seek 5 + 300 counts @12:48:03.93 Forearm.reconfigure :loose @12:48:03.98 Hand.close @12:48:04.55 SE.seek :down @12:48:04.76
Forearm.seek :retract @12:48:06.12 SE.stop @12:48:06.18
Elbow.seek CC @12:48:07.89 Elbow.reconfigure :loose @12:48:08.00 Forearm.reconfigure :tight @12:48:08.04 Forearm.seek CC @12:48:09.29 CC.seek :closed @12:48:13.81 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.26 @12:48:13.82 Hand.open @12:48:14.49 Forearm.reconfigure :loose @12:48:14.53 Forearm.seek :retract @12:48:15.56 collectSample 1000ml w/bubble point of 24psi @12:48:15.57 Priming 7ml @12:48:15.58 Intake.select :close @12:48:15.59 Exhaust.select :close @12:48:15.60 SS.hold @12:48:15.81 Calibrating pressure and temperature sensors @12:48:15.84 CTV.dial :air @12:48:15.91 SSV.dial :spit,avoiding: :exhaust @12:48:15.99 SS.reconfigure :fast @12:48:16.04 SS.seek 5ml @12:48:18.94 SSV.dial CBV @12:48:18.96
CBV.dial SSV @12:48:20.30 delay 3.8 seconds @12:48:24.23 Sensor limits: Intake max 102.3psia; Outlet max 94.8psia @12:48:24.24 SSV.dial :spit @12:48:25.58 SS.seek 0ml @12:48:28.36 SSV.dial CBV @12:48:29.67 CTV.dial :intake @12:48:30.46 SSV.dial CBV @12:48:30.48
CBV.dial SSV @12:48:30.65 Allowing 40s for 7.0ml of remaining 7.0ml @12:48:30.66 Intake.select :open @12:48:32.82 Intake @14.5psia, vacuum from 9.0 to 12.0psi @12:48:32.84 SS.reconfigure :inhaling 2.20ml/s @12:48:32.89 SS.seek 7ml @12:48:39.68 Stroke completed -- waiting for pressure to equalize ... @12:48:39.69 SS.reconfigure :exhale @12:48:39.74 SS.hold @12:48:45.79 Intake.select :close @12:48:45.95 SSV.dial :exhaust @12:48:46.70 Exhaust.select :open @12:48:46.82 SS.seek 0ml @12:48:50.85 Exhaust.select :close @12:48:50.96 Sampling 1000.0ml @12:48:50.97 Intake.select :close @12:48:50.98 Exhaust.select :close @12:48:51.00 SS.reconfigure :default @12:48:51.04 SS.hold @12:48:51.24 CTV.dial :intake @12:48:51.32 SSV.dial CBV @12:48:51.34
CBV.dial SSV @12:48:52.13 Allowing 130s for 25.0ml of remaining 1000.0ml @12:48:52.14 Intake.select :open @12:48:54.30 Intake @15.1psia, vacuum from 9.6 to 12.6psi @12:48:54.31 SS.reconfigure :inhaling 2.20ml/s @12:48:54.36 SS.seek 25ml @12:49:02.43 Vacuum too high -- waiting up to 40 seconds @12:49:02.44 SS.reconfigure :exhale @12:49:02.50 SS.hold @12:49:08.51 Intake @17.5psia, vacuum from 10.0 to 13.0psi @12:49:08.52 SS.reconfigure :inhaling 1.76ml/s @12:49:08.58 SS.seek 25ml @12:49:11.56 Vacuum too high -- waiting up to 40 seconds @12:49:11.57 SS.reconfigure :exhale @12:49:11.62 SS.hold @12:49:13.24 Intake @16.6psia, vacuum from 10.0 to 13.0psi @12:49:13.25 SS.reconfigure :inhaling 1.41ml/s @12:49:13.30 SS.seek 25ml @12:49:19.15 Vacuum too high -- waiting up to 40 seconds @12:49:19.16 SS.reconfigure :exhale @12:49:19.21 SS.hold @12:49:22.96 Intake @17.6psia, vacuum from 10.0 to 13.0psi @12:49:22.98 SS.reconfigure :inhaling 1.13ml/s @12:49:23.03 SS.seek 25ml @12:49:26.54 Stroke completed -- waiting for pressure to equalize ... @12:49:26.55 SS.reconfigure :exhale @12:49:26.60 SS.hold @12:49:47.00 Intake.select :close @12:49:47.16 SSV.dial :exhaust @12:49:47.92 Exhaust.select :open @12:49:48.04 SS.seek 0ml @12:49:56.86 Exhaust.select :close @12:49:56.96 SSV.dial CBV @12:49:57.00
CBV.dial SSV @12:49:57.75 Allowing 130s for 25.0ml of remaining 975.0ml @12:49:57.76 Intake.select :open @12:49:59.93 Intake @15.8psia, vacuum from 10.0 to 13.0psi @12:49:59.94 SS.reconfigure :inhaling 1.13ml/s @12:49:59.99 SS.seek 25ml @12:50:24.81 Stroke completed -- waiting for pressure to equalize ... @12:50:24.82 SS.reconfigure :exhale @12:50:24.87 SS.hold @12:50:51.34 Intake.select :close @12:50:51.49 SSV.dial :exhaust @12:50:52.26 Exhaust.select :open @12:50:52.37 SS.seek 0ml @12:51:01.20 Exhaust.select :close @12:51:01.31 SSV.dial CBV @12:51:01.33
CBV.dial SSV @12:51:02.09 Allowing 130s for 25.0ml of remaining 950.0ml @12:51:02.10 Intake.select :open @12:51:04.27 Intake @15.9psia, vacuum from 10.0 to 13.0psi @12:51:04.28 SS.reconfigure :inhaling 1.13ml/s @12:51:04.33 SS.seek 25ml @12:51:29.17 Stroke completed -- waiting for pressure to equalize ... @12:51:29.18 SS.reconfigure :exhale @12:51:29.22 SS.hold @12:51:55.76 Intake.select :close @12:51:55.91 SSV.dial :exhaust @12:51:56.66 Exhaust.select :open @12:51:56.77 SS.seek 0ml @12:52:05.59 Exhaust.select :close @12:52:05.70 SSV.dial CBV @12:52:05.72
CBV.dial SSV @12:52:06.48 Allowing 130s for 25.0ml of remaining 925.0ml @12:52:06.49 Intake.select :open @12:52:08.66 Intake @15.9psia, vacuum from 10.0 to 13.0psi @12:52:08.68 SS.reconfigure :inhaling 1.13ml/s @12:52:08.73 SS.seek 25ml @12:52:33.62 Stroke completed -- waiting for pressure to equalize ... @12:52:33.63 SS.reconfigure :exhale @12:52:33.68 SS.hold @12:52:56.19 Intake.select :close @12:52:56.34 SSV.dial :exhaust @12:52:57.10 Exhaust.select :open @12:52:57.22 SS.seek 0ml @12:53:06.26 Exhaust.select :close @12:53:06.37 SSV.dial CBV @12:53:06.39
CBV.dial SSV @12:53:07.17 Allowing 130s for 25.0ml of remaining 900.0ml @12:53:07.18 Intake.select :open @12:53:09.35 Intake @16.0psia, vacuum from 10.0 to 13.0psi @12:53:09.36 SS.reconfigure :inhaling 1.13ml/s @12:53:09.41 SS.seek 25ml @12:53:33.80 Stroke completed -- waiting for pressure to equalize ... @12:53:33.81 SS.reconfigure :exhale @12:53:33.86 SS.hold @12:53:58.82 Intake.select :close @12:53:58.97 SSV.dial :exhaust @12:53:59.73 Exhaust.select :open @12:53:59.84 SS.seek 0ml @12:54:08.85 Exhaust.select :close @12:54:08.96 SSV.dial CBV @12:54:09.00
CBV.dial SSV @12:54:09.74 Allowing 130s for 25.0ml of remaining 875.0ml @12:54:09.76 Intake.select :open @12:54:11.93 Intake @15.9psia, vacuum from 10.0 to 13.0psi @12:54:11.94 SS.reconfigure :inhaling 1.13ml/s @12:54:11.99 SS.seek 25ml @12:54:36.41 Stroke completed -- waiting for pressure to equalize ... @12:54:36.42 SS.reconfigure :exhale @12:54:36.47 SS.hold @12:54:56.88 Intake.select :close @12:54:57.04 SSV.dial :exhaust @12:54:57.79 Exhaust.select :open @12:54:57.90 SS.seek 0ml @12:55:06.71 Exhaust.select :close @12:55:06.83 SSV.dial CBV @12:55:06.86
CBV.dial SSV @12:55:07.67 Allowing 130s for 25.0ml of remaining 850.0ml @12:55:07.68 Intake.select :open @12:55:09.85 Intake @16.0psia, vacuum from 10.0 to 13.0psi @12:55:09.87 SS.reconfigure :inhaling 1.13ml/s @12:55:09.92 SS.seek 25ml @12:55:34.69 Stroke completed -- waiting for pressure to equalize ... @12:55:34.70 SS.reconfigure :exhale @12:55:34.75 SS.hold @12:55:57.15 Intake.select :close @12:55:57.30 SSV.dial :exhaust @12:55:58.07 Exhaust.select :open @12:55:58.18 SS.seek 0ml @12:56:07.11 Exhaust.select :close @12:56:07.22 SSV.dial CBV @12:56:07.26
CBV.dial SSV @12:56:08.01 Allowing 130s for 25.0ml of remaining 825.0ml @12:56:08.02 Intake.select :open @12:56:10.18 Intake @15.9psia, vacuum from 10.0 to 13.0psi @12:56:10.19 SS.reconfigure :inhaling 1.13ml/s @12:56:10.24 SS.seek 25ml @12:56:34.58 Stroke completed -- waiting for pressure to equalize ... @12:56:34.59 SS.reconfigure :exhale @12:56:34.64 SS.hold @12:56:55.31 Intake.select :close @12:56:55.47 SSV.dial :exhaust @12:56:56.23 Exhaust.select :open @12:56:56.35 SS.seek 0ml @12:57:05.11 Exhaust.select :close @12:57:05.22 SSV.dial CBV @12:57:05.25
CBV.dial SSV @12:57:06.01 Allowing 130s for 25.0ml of remaining 800.0ml @12:57:06.02 Intake.select :open @12:57:08.18 Intake @16.0psia, vacuum from 10.0 to 13.0psi @12:57:08.19 SS.reconfigure :inhaling 1.13ml/s @12:57:08.24 SS.seek 25ml @12:57:31.01 Vacuum too high -- waiting up to 40 seconds @12:57:31.02 SS.reconfigure :exhale @12:57:31.08 SS.hold @12:57:37.87 Intake @16.2psia, vacuum from 10.0 to 13.0psi @12:57:37.89 SS.reconfigure :inhaling 0.91ml/s @12:57:37.94 SS.seek 25ml @12:57:40.56 Stroke completed -- waiting for pressure to equalize ... @12:57:40.57 SS.reconfigure :exhale @12:57:40.62 SS.hold @12:57:51.78 Intake.select :close @12:57:51.93 SSV.dial :exhaust @12:57:52.69 Exhaust.select :open @12:57:52.81 SS.seek 0ml @12:58:01.83 Exhaust.select :close @12:58:01.94 SSV.dial CBV @12:58:01.97
CBV.dial SSV @12:58:02.72 Allowing 130s for 25.0ml of remaining 775.0ml @12:58:02.74 Intake.select :open @12:58:05.33 Intake @16.1psia, vacuum from 10.0 to 13.0psi @12:58:05.35 SS.reconfigure :inhaling 0.91ml/s @12:58:05.40 SS.seek 25ml @12:58:34.91 Stroke completed -- waiting for pressure to equalize ... @12:58:34.92 SS.reconfigure :exhale @12:58:34.97 SS.hold @12:58:49.85 Intake.select :close @12:58:50.00 SSV.dial :exhaust @12:58:50.78 Exhaust.select :open @12:58:50.89 SS.seek 0ml @12:58:59.61 Exhaust.select :close @12:58:59.72 SSV.dial CBV @12:58:59.74
CBV.dial SSV @12:59:00.52 Allowing 130s for 25.0ml of remaining 750.0ml @12:59:00.53 Intake.select :open @12:59:02.70 Intake @16.1psia, vacuum from 10.0 to 13.0psi @12:59:02.71 SS.reconfigure :inhaling 0.91ml/s @12:59:02.76 SS.seek 25ml @12:59:32.50 Stroke completed -- waiting for pressure to equalize ... @12:59:32.51 SS.reconfigure :exhale @12:59:32.56 SS.hold @12:59:49.07 Intake.select :close @12:59:49.22 SSV.dial :exhaust @12:59:49.99 Exhaust.select :open @12:59:50.10 SS.seek 0ml @12:59:59.08 Exhaust.select :close @12:59:59.19 SSV.dial CBV @12:59:59.21
CBV.dial SSV @12:59:59.99 Allowing 130s for 25.0ml of remaining 725.0ml @13:00:00.00 Intake.select :open @13:00:02.17 Intake @16.1psia, vacuum from 10.0 to 13.0psi @13:00:02.18 SS.reconfigure :inhaling 0.91ml/s @13:00:02.23 SS.seek 25ml @13:00:31.73 Stroke completed -- waiting for pressure to equalize ... @13:00:31.74 SS.reconfigure :exhale @13:00:31.79 SS.hold @13:00:48.17 Intake.select :close @13:00:48.33 SSV.dial :exhaust @13:00:49.09 Exhaust.select :open @13:00:49.21 SS.seek 0ml @13:00:58.28 Exhaust.select :close @13:00:58.39 SSV.dial CBV @13:00:58.41
CBV.dial SSV @13:00:59.17 Allowing 130s for 25.0ml of remaining 700.0ml @13:00:59.19 Intake.select :open @13:01:01.35 Intake @16.0psia, vacuum from 10.0 to 13.0psi @13:01:01.37 SS.reconfigure :inhaling 0.91ml/s @13:01:01.42 SS.seek 25ml @13:01:30.61 Stroke completed -- waiting for pressure to equalize ... @13:01:30.62 SS.reconfigure :exhale @13:01:30.66 SS.hold @13:01:49.03 Intake.select :close @13:01:49.19 SSV.dial :exhaust @13:01:49.95 Exhaust.select :open @13:01:50.07 SS.seek 0ml @13:01:58.81 Exhaust.select :close @13:01:58.92 SSV.dial CBV @13:01:58.95
CBV.dial SSV @13:01:59.72 Allowing 130s for 25.0ml of remaining 675.0ml @13:01:59.73 Intake.select :open @13:02:01.90 Intake @16.1psia, vacuum from 10.0 to 13.0psi @13:02:01.92 SS.reconfigure :inhaling 0.91ml/s @13:02:01.97 SS.seek 25ml @13:02:31.68 Stroke completed -- waiting for pressure to equalize ... @13:02:31.69 SS.reconfigure :exhale @13:02:31.74 SS.hold @13:02:50.09 Intake.select :close @13:02:50.68 SSV.dial :exhaust @13:02:51.44 Exhaust.select :open @13:02:51.55 SS.seek 0ml @13:03:00.33 Exhaust.select :close @13:03:00.43 SSV.dial CBV @13:03:00.45
CBV.dial SSV @13:03:01.23 Allowing 130s for 25.0ml of remaining 650.0ml @13:03:01.25 Intake.select :open @13:03:03.42 Intake @16.1psia, vacuum from 10.0 to 13.0psi @13:03:03.43 SS.reconfigure :inhaling 0.91ml/s @13:03:03.48 SS.seek 25ml @13:03:33.25 Stroke completed -- waiting for pressure to equalize ... @13:03:33.26 SS.reconfigure :exhale @13:03:33.30 SS.hold @13:03:51.67 Intake.select :close @13:03:51.82 SSV.dial :exhaust @13:03:52.58 Exhaust.select :open @13:03:52.69 SS.seek 0ml @13:04:01.42 Exhaust.select :close @13:04:01.53 SSV.dial CBV @13:04:01.55
CBV.dial SSV @13:04:02.33 Allowing 130s for 25.0ml of remaining 625.0ml @13:04:02.34 Intake.select :open @13:04:04.50 Intake @16.0psia, vacuum from 10.0 to 13.0psi @13:04:04.87 SS.reconfigure :inhaling 0.91ml/s @13:04:04.99 SS.seek 25ml @13:04:34.73 Stroke completed -- waiting for pressure to equalize ... @13:04:34.74 SS.reconfigure :exhale @13:04:34.79 SS.hold @13:04:55.19 Intake.select :close @13:04:55.35 SSV.dial :exhaust @13:04:56.11 Exhaust.select :open @13:04:56.23 SS.seek 0ml @13:05:05.03 Exhaust.select :close @13:05:05.13 SSV.dial CBV @13:05:05.15
CBV.dial SSV @13:05:05.92 Allowing 130s for 25.0ml of remaining 600.0ml @13:05:05.93 Intake.select :open @13:05:08.10 Intake @16.0psia, vacuum from 10.0 to 13.0psi @13:05:08.11 SS.reconfigure :inhaling 0.91ml/s @13:05:08.16 SS.seek 25ml @13:05:34.13 Vacuum too high -- waiting up to 40 seconds @13:05:34.14 SS.reconfigure :exhale @13:05:34.19 SS.hold @13:05:42.05 Intake @16.5psia, vacuum from 10.0 to 13.0psi @13:05:42.07 SS.reconfigure :inhaling 0.73ml/s @13:05:42.12 SS.seek 25ml @13:05:47.07 Stroke completed -- waiting for pressure to equalize ... @13:05:47.08 SS.reconfigure :exhale @13:05:47.13 SS.hold @13:06:01.93 Intake.select :close @13:06:02.09 SSV.dial :exhaust @13:06:02.85 Exhaust.select :open @13:06:02.97 SS.seek 0ml @13:06:11.65 Exhaust.select :close @13:06:11.76 SSV.dial CBV @13:06:11.78
CBV.dial SSV @13:06:12.56 Allowing 130s for 25.0ml of remaining 575.0ml @13:06:12.57 Intake.select :open @13:06:14.74 Intake @15.9psia, vacuum from 10.0 to 13.0psi @13:06:14.76 SS.reconfigure :inhaling 0.73ml/s @13:06:14.81 SS.seek 25ml @13:06:51.21 Stroke completed -- waiting for pressure to equalize ... @13:06:51.22 SS.reconfigure :exhale @13:06:51.27 SS.hold @13:07:09.63 Intake.select :close @13:07:09.79 SSV.dial :exhaust @13:07:10.56 Exhaust.select :open @13:07:10.67 SS.seek 0ml @13:07:19.43 Exhaust.select :close @13:07:19.96 SSV.dial CBV @13:07:19.98
CBV.dial SSV @13:07:20.76 Allowing 130s for 25.0ml of remaining 550.0ml @13:07:20.77 Intake.select :open @13:07:22.94 Intake @16.0psia, vacuum from 10.0 to 13.0psi @13:07:22.96 SS.reconfigure :inhaling 0.73ml/s @13:07:23.01 SS.seek 25ml @13:07:59.41 Stroke completed -- waiting for pressure to equalize ... @13:07:59.42 SS.reconfigure :exhale @13:07:59.47 SS.hold @13:08:15.81 Intake.select :close @13:08:15.97 SSV.dial :exhaust @13:08:16.73 Exhaust.select :open @13:08:16.84 SS.seek 0ml @13:08:25.62 Exhaust.select :close @13:08:25.72 SSV.dial CBV @13:08:25.74
CBV.dial SSV @13:08:26.51 Allowing 130s for 25.0ml of remaining 525.0ml @13:08:26.52 Intake.select :open @13:08:28.69 Intake @16.1psia, vacuum from 10.0 to 13.0psi @13:08:28.70 SS.reconfigure :inhaling 0.73ml/s @13:08:28.75 SS.seek 25ml @13:09:05.25 Stroke completed -- waiting for pressure to equalize ... @13:09:05.26 SS.reconfigure :exhale @13:09:05.31 SS.hold @13:09:23.93 Intake.select :close @13:09:24.08 SSV.dial :exhaust @13:09:24.83 Exhaust.select :open @13:09:24.95 SS.seek 0ml @13:09:33.71 Exhaust.select :close @13:09:33.82 SSV.dial CBV @13:09:33.84
CBV.dial SSV @13:09:34.62 Allowing 130s for 25.0ml of remaining 500.0ml @13:09:34.63 Intake.select :open @13:09:36.79 Intake @16.1psia, vacuum from 10.0 to 13.0psi @13:09:36.80 SS.reconfigure :inhaling 0.73ml/s @13:09:36.86 SS.seek 25ml @13:10:13.41 Stroke completed -- waiting for pressure to equalize ... @13:10:13.42 SS.reconfigure :exhale @13:10:13.47 SS.hold @13:10:34.04 Intake.select :close @13:10:34.19 SSV.dial :exhaust @13:10:34.96 Exhaust.select :open @13:10:35.07 SS.seek 0ml @13:10:43.85 Exhaust.select :close @13:10:43.95 SSV.dial CBV @13:10:43.99
CBV.dial SSV @13:10:44.72 Allowing 130s for 25.0ml of remaining 475.0ml @13:10:44.74 Intake.select :open @13:10:46.90 Intake @16.1psia, vacuum from 10.0 to 13.0psi @13:10:46.92 SS.reconfigure :inhaling 0.73ml/s @13:10:46.97 SS.seek 25ml @13:11:21.49 Vacuum too high -- waiting up to 40 seconds @13:11:21.51 SS.reconfigure :exhale @13:11:21.56 SS.hold @13:11:30.29 Intake @17.2psia, vacuum from 10.0 to 13.0psi @13:11:30.31 SS.reconfigure :inhaling 0.59ml/s @13:11:30.36 SS.seek 25ml @13:11:32.75 Stroke completed -- waiting for pressure to equalize ... @13:11:32.76 SS.reconfigure :exhale @13:11:32.81 SS.hold @13:11:45.48 Intake.select :close @13:11:45.63 SSV.dial :exhaust @13:11:46.39 Exhaust.select :open @13:11:46.51 SS.seek 0ml @13:11:55.19 Exhaust.select :close @13:11:55.72 SSV.dial CBV @13:11:55.77
CBV.dial SSV @13:11:56.51 Allowing 130s for 25.0ml of remaining 450.0ml @13:11:56.52 Intake.select :open @13:11:58.68 Intake @16.1psia, vacuum from 10.0 to 13.0psi @13:11:58.70 SS.reconfigure :inhaling 0.59ml/s @13:11:58.75 SS.seek 25ml @13:12:43.47 Stroke completed -- waiting for pressure to equalize ... @13:12:43.48 SS.reconfigure :exhale @13:12:43.53 SS.hold @13:13:01.93 Intake.select :close @13:13:02.09 SSV.dial :exhaust @13:13:02.85 Exhaust.select :open @13:13:02.96 SS.seek 0ml @13:13:11.78 Exhaust.select :close @13:13:11.89 SSV.dial CBV @13:13:11.92
CBV.dial SSV @13:13:12.69 Allowing 130s for 25.0ml of remaining 425.0ml @13:13:12.70 Intake.select :open @13:13:14.87 Intake @16.1psia, vacuum from 10.0 to 13.0psi @13:13:14.88 SS.reconfigure :inhaling 0.59ml/s @13:13:14.93 SS.seek 25ml @13:13:59.89 Stroke completed -- waiting for pressure to equalize ... @13:14:00.25 SS.reconfigure :exhale @13:14:00.36 SS.hold @13:14:18.72 Intake.select :close @13:14:18.88 SSV.dial :exhaust @13:14:19.64 Exhaust.select :open @13:14:19.76 SS.seek 0ml @13:14:28.72 Exhaust.select :close @13:14:28.83 SSV.dial CBV @13:14:28.85
CBV.dial SSV @13:14:29.61 Allowing 130s for 25.0ml of remaining 400.0ml @13:14:29.62 Intake.select :open @13:14:31.79 Intake @16.1psia, vacuum from 10.0 to 13.0psi @13:14:31.80 SS.reconfigure :inhaling 0.59ml/s @13:14:31.85 SS.seek 25ml @13:15:16.36 Stroke completed -- waiting for pressure to equalize ... @13:15:16.37 SS.reconfigure :exhale @13:15:16.42 SS.hold @13:15:37.13 Intake.select :close @13:15:37.29 SSV.dial :exhaust @13:15:38.09 Exhaust.select :open @13:15:38.21 SS.seek 0ml @13:15:47.20 Exhaust.select :close @13:15:47.31 SSV.dial CBV @13:15:47.34
CBV.dial SSV @13:15:48.11 Allowing 130s for 25.0ml of remaining 375.0ml @13:15:48.12 Intake.select :open @13:15:50.29 Intake @16.2psia, vacuum from 10.0 to 13.0psi @13:15:50.31 SS.reconfigure :inhaling 0.59ml/s @13:15:50.36 SS.seek 25ml @13:16:32.80 Vacuum too high -- waiting up to 40 seconds @13:16:32.81 SS.reconfigure :exhale @13:16:32.87 SS.hold @13:16:39.57 Intake @16.4psia, vacuum from 10.0 to 13.0psi @13:16:39.59 SS.reconfigure :inhaling 0.47ml/s @13:16:39.64 SS.seek 25ml @13:16:42.10 Stroke completed -- waiting for pressure to equalize ... @13:16:42.12 SS.reconfigure :exhale @13:16:42.16 SS.hold @13:16:56.55 Intake.select :close @13:16:56.70 SSV.dial :exhaust @13:16:57.51 Exhaust.select :open @13:16:57.63 SS.seek 0ml @13:17:06.40 Exhaust.select :close @13:17:06.88 SSV.dial CBV @13:17:06.91
CBV.dial SSV @13:17:07.67 Allowing 130s for 25.0ml of remaining 350.0ml @13:17:07.68 Intake.select :open @13:17:09.85 Intake @16.3psia, vacuum from 10.0 to 13.0psi @13:17:09.87 SS.reconfigure :inhaling 0.47ml/s @13:17:09.92 SS.seek 25ml @13:18:05.56 Stroke completed -- waiting for pressure to equalize ... @13:18:05.57 SS.reconfigure :exhale @13:18:05.62 SS.hold @13:18:23.97 Intake.select :close @13:18:24.13 SSV.dial :exhaust @13:18:24.89 Exhaust.select :open @13:18:25.00 SS.seek 0ml @13:18:33.81 Exhaust.select :close @13:18:33.91 SSV.dial CBV @13:18:33.93
CBV.dial SSV @13:18:34.70 Allowing 130s for 25.0ml of remaining 325.0ml @13:18:34.71 Intake.select :open @13:18:36.88 Intake @16.2psia, vacuum from 10.0 to 13.0psi @13:18:36.89 SS.reconfigure :inhaling 0.47ml/s @13:18:36.94 SS.seek 25ml @13:19:32.43 Stroke completed -- waiting for pressure to equalize ... @13:19:32.79 SS.reconfigure :exhale @13:19:32.91 SS.hold @13:19:53.16 Intake.select :close @13:19:53.31 SSV.dial :exhaust @13:19:54.08 Exhaust.select :open @13:19:54.19 SS.seek 0ml @13:20:03.02 Exhaust.select :close @13:20:03.13 SSV.dial CBV @13:20:03.17
CBV.dial SSV @13:20:03.92 Allowing 130s for 25.0ml of remaining 300.0ml @13:20:03.93 Intake.select :open @13:20:06.09 Intake @16.3psia, vacuum from 10.0 to 13.0psi @13:20:06.11 SS.reconfigure :inhaling 0.47ml/s @13:20:06.17 SS.seek 25ml @13:21:01.63 Stroke completed -- waiting for pressure to equalize ... @13:21:01.64 SS.reconfigure :exhale @13:21:01.69 SS.hold @13:21:24.39 Intake.select :close @13:21:24.54 SSV.dial :exhaust @13:21:25.30 Exhaust.select :open @13:21:25.42 SS.seek 0ml @13:21:34.23 Exhaust.select :close @13:21:34.34 SSV.dial CBV @13:21:34.36
CBV.dial SSV @13:21:35.12 Allowing 130s for 25.0ml of remaining 275.0ml @13:21:35.13 Intake.select :open @13:21:37.30 Intake @16.3psia, vacuum from 10.0 to 13.0psi @13:21:37.31 SS.reconfigure :inhaling 0.47ml/s @13:21:37.36 SS.seek 25ml @13:22:32.96 Stroke completed -- waiting for pressure to equalize ... @13:22:32.97 SS.reconfigure :exhale @13:22:33.02 SS.hold @13:22:57.69 Intake.select :close @13:22:57.84 SSV.dial :exhaust @13:22:58.60 Exhaust.select :open @13:22:58.71 SS.seek 0ml @13:23:07.57 Exhaust.select :close @13:23:07.67 SSV.dial CBV @13:23:07.69
CBV.dial SSV @13:23:08.46 Allowing 130s for 25.0ml of remaining 250.0ml @13:23:08.47 Intake.select :open @13:23:10.64 Intake @16.3psia, vacuum from 10.0 to 13.0psi @13:23:10.65 SS.reconfigure :inhaling 0.47ml/s @13:23:10.70 SS.seek 25ml @13:24:06.58 Stroke completed -- waiting for pressure to equalize ... @13:24:06.59 SS.reconfigure :exhale @13:24:06.64 SS.hold @13:24:31.57 Intake.select :close @13:24:31.72 SSV.dial :exhaust @13:24:32.47 Exhaust.select :open @13:24:32.59 SS.seek 0ml @13:24:41.41 Exhaust.select :close @13:24:41.52 SSV.dial CBV @13:24:41.55
CBV.dial SSV @13:24:42.32 Allowing 130s for 25.0ml of remaining 225.0ml @13:24:42.33 Intake.select :open @13:24:44.50 Intake @16.4psia, vacuum from 10.0 to 13.0psi @13:24:44.52 SS.reconfigure :inhaling 0.47ml/s @13:24:44.57 SS.seek 25ml @13:25:37.45 Vacuum too high -- waiting up to 40 seconds @13:25:37.46 SS.reconfigure :exhale @13:25:37.51 SS.hold @13:25:50.46 SS.reconfigure :inhaling 0.38ml/s @13:25:50.51 SS.seek 25ml @13:25:53.75 Stroke completed -- waiting for pressure to equalize ... @13:25:53.76 SS.reconfigure :exhale @13:25:53.81 SS.hold @13:26:03.57 Intake.select :close @13:26:03.73 SSV.dial :exhaust @13:26:04.92 Exhaust.select :open @13:26:05.04 SS.seek 0ml @13:26:13.85 Exhaust.select :close @13:26:13.95 SSV.dial CBV @13:26:13.99
CBV.dial SSV @13:26:14.75 Allowing 130s for 25.0ml of remaining 200.0ml @13:26:14.77 Intake.select :open @13:26:16.93 Intake @16.7psia, vacuum from 10.0 to 13.0psi @13:26:16.95 SS.reconfigure :inhaling 0.38ml/s @13:26:17.00 SS.seek 25ml @13:27:25.42 Stroke completed -- waiting for pressure to equalize ... @13:27:25.43 SS.reconfigure :exhale @13:27:25.48 SS.hold @13:27:47.93 Intake.select :close @13:27:48.09 SSV.dial :exhaust @13:27:48.84 Exhaust.select :open @13:27:48.96 SS.seek 0ml @13:27:57.98 Exhaust.select :close @13:27:58.09 SSV.dial CBV @13:27:58.13
CBV.dial SSV @13:27:58.86 Allowing 130s for 25.0ml of remaining 175.0ml @13:27:58.87 Intake.select :open @13:28:01.04 Intake @16.4psia, vacuum from 10.0 to 13.0psi @13:28:01.06 SS.reconfigure :inhaling 0.38ml/s @13:28:01.11 SS.seek 25ml @13:29:09.17 Stroke completed -- waiting for pressure to equalize ... @13:29:09.18 SS.reconfigure :exhale @13:29:09.23 SS.hold @13:29:35.81 Intake.select :close @13:29:35.97 SSV.dial :exhaust @13:29:36.74 Exhaust.select :open @13:29:36.85 SS.seek 0ml @13:29:46.36 Exhaust.select :close @13:29:46.47 SSV.dial CBV @13:29:46.49
CBV.dial SSV @13:29:47.27 Allowing 130s for 25.0ml of remaining 150.0ml @13:29:47.28 Intake.select :open @13:29:49.44 Intake @16.3psia, vacuum from 10.0 to 13.0psi @13:29:49.46 SS.reconfigure :inhaling 0.38ml/s @13:29:49.51 SS.seek 25ml @13:30:58.06 Stroke completed -- waiting for pressure to equalize ... @13:30:58.07 SS.reconfigure :exhale @13:30:58.12 SS.hold @13:31:26.71 Intake.select :close @13:31:26.86 SSV.dial :exhaust @13:31:27.97 Exhaust.select :open @13:31:28.16 SS.seek 0ml @13:31:36.96 Exhaust.select :close @13:31:37.08 SSV.dial CBV @13:31:37.10
CBV.dial SSV @13:31:37.87 Allowing 130s for 25.0ml of remaining 125.0ml @13:31:37.89 Intake.select :open @13:31:40.05 Intake @16.3psia, vacuum from 10.0 to 13.0psi @13:31:40.07 SS.reconfigure :inhaling 0.38ml/s @13:31:40.12 SS.seek 25ml @13:32:48.24 Stroke completed -- waiting for pressure to equalize ... @13:32:48.25 SS.reconfigure :exhale @13:32:48.30 SS.hold @13:33:16.91 Intake.select :close @13:33:17.44 SSV.dial :exhaust @13:33:18.20 Exhaust.select :open @13:33:18.31 SS.seek 0ml @13:33:27.12 Exhaust.select :close @13:33:27.23 SSV.dial CBV @13:33:27.25
CBV.dial SSV @13:33:28.01 Allowing 130s for 25.0ml of remaining 100.0ml @13:33:28.02 Intake.select :open @13:33:30.19 Intake @16.4psia, vacuum from 10.0 to 13.0psi @13:33:30.21 SS.reconfigure :inhaling 0.38ml/s @13:33:30.26 SS.seek 25ml @13:34:39.24 Stroke completed -- waiting for pressure to equalize ... @13:34:39.25 SS.reconfigure :exhale @13:34:39.30 SS.hold @13:35:12.42 Intake.select :close @13:35:12.57 SSV.dial :exhaust @13:35:13.34 Exhaust.select :open @13:35:13.45 SS.seek 0ml @13:35:22.49 Exhaust.select :close @13:35:22.60 SSV.dial CBV @13:35:22.64
CBV.dial SSV @13:35:23.40 Allowing 130s for 25.0ml of remaining 75.0ml @13:35:23.41 Intake.select :open @13:35:25.58 Intake @16.4psia, vacuum from 10.0 to 13.0psi @13:35:25.59 SS.reconfigure :inhaling 0.38ml/s @13:35:25.64 SS.seek 25ml @13:36:33.63 Stroke completed -- waiting for pressure to equalize ... @13:36:33.64 SS.reconfigure :exhale @13:36:33.69 SS.hold @13:37:10.54 Intake.select :close @13:37:10.70 SSV.dial :exhaust @13:37:11.46 Exhaust.select :open @13:37:11.57 SS.seek 0ml @13:37:20.68 Exhaust.select :close @13:37:20.78 SSV.dial CBV @13:37:20.80
CBV.dial SSV @13:37:21.58 Allowing 130s for 25.0ml of remaining 50.0ml @13:37:21.60 Intake.select :open @13:37:23.76 Intake @16.4psia, vacuum from 10.0 to 13.0psi @13:37:23.78 SS.reconfigure :inhaling 0.38ml/s @13:37:23.83 SS.seek 25ml @13:38:31.89 Stroke completed -- waiting for pressure to equalize ... @13:38:31.90 SS.reconfigure :exhale @13:38:31.95 SS.hold @13:39:08.73 Intake.select :close @13:39:08.89 SSV.dial :exhaust @13:39:09.64 Exhaust.select :open @13:39:09.76 SS.seek 0ml @13:39:18.78 Exhaust.select :close @13:39:18.89 SSV.dial CBV @13:39:18.93
CBV.dial SSV @13:39:19.66 Allowing 130s for 25.0ml of remaining 25.0ml @13:39:19.67 Intake.select :open @13:39:21.84 Intake @16.4psia, vacuum from 10.0 to 13.0psi @13:39:21.85 SS.reconfigure :inhaling 0.38ml/s @13:39:21.90 SS.seek 24.99ml @13:40:30.44 Stroke completed -- waiting for pressure to equalize ... @13:40:30.45 SS.reconfigure :exhale @13:40:30.49 SS.hold @13:41:09.40 Intake.select :close @13:41:09.55 SSV.dial :exhaust @13:41:10.32 Exhaust.select :open @13:41:10.44 SS.seek 0ml @13:41:19.31 Exhaust.select :close @13:41:19.42 Sampled 1000.0ml @13:41:19.44 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) Intake.select :close @13:41:19.51 CSV.dial :waste @13:41:19.67 SSV.dial CBV,avoiding: :exhaust @13:41:19.69 CS.seek 0ml @13:41:19.73
CBV.dial SSV @13:41:19.82 CSV.dial :air @13:41:20.55 CS.seek 10ml @13:41:21.64
CTV.dial :air @13:41:22.44 delay 15 seconds @13:41:37.46 SSV.dial :spit,avoiding: :exhaust @13:41:38.77 CTV.dial CSV,avoiding: :intake @13:41:38.80
SS.seek 0ml @13:41:38.93 SSV.dial CBV,avoiding: :exhaust @13:41:45.19 SSV.dial between CBV and plug @13:41:46.03 CBV.dial between SSV and waste2 @13:41:46.48 CSV.dial between air and CTV @13:41:46.81 CS.seek 8.252ml @13:41:51.73 CTV.dial CSV,avoiding: :air @13:41:51.77
CSV.dial CTV @13:41:52.11 CS.reconfigure :pressurize @13:41:52.17 CS.seek 0ml @13:42:06.01 BubblePoint: Intake @24.2psia, Outlet @0.9psia @13:42:06.02 CBV.dial SSV @13:42:06.36 SS.reconfigure :default @13:42:06.41 SS.seek 10ml @13:42:12.05 delay 15 seconds @13:42:27.18 Evacuated: Intake @23.7psia, Outlet @23.3psia @13:42:27.19 SSV.dial :spit,avoiding: :exhaust @13:42:28.22 SS.reconfigure :exhale @13:42:28.27 SS.seek 0ml @13:42:33.25 SS.reconfigure :fast @13:42:33.30 SS.seek 5ml @13:42:36.07 SSV.dial CBV,avoiding: :exhaust @13:42:36.09
CBV.dial SSV @13:42:37.42 CTV.dial :air,avoiding: :intake @13:42:38.77 CSV.dial CTV @13:42:38.80
SS.seek 10ml @13:42:39.93 CSV.dial :air @13:42:40.39 CS.reconfigure :fast @13:42:40.44 CS.seek 0ml @13:42:41.28
SSV.dial :spit,avoiding: :exhaust @13:42:42.60 SS.reconfigure :exhale @13:42:42.65 SS.seek 0ml @13:42:47.63 SS.reconfigure :fast @13:42:47.68 SS.seek 5ml @13:42:48.31 CS.stop @13:42:50.62 SSV.dial CBV,avoiding: :exhaust @13:42:50.64
CBV.dial SSV @13:42:53.01 SSV.dial :spit,avoiding: :exhaust @13:42:54.32 SS.reconfigure :default @13:42:54.36 SS.seek 0ml @13:42:57.51 SS.stop @13:42:57.56 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @13:42:57.57 Intake.select :close @13:42:57.63 CSV.dial :waste @13:42:57.76 SSV.dial CBV,avoiding: :exhaust @13:42:57.78
CBV.dial SSV @13:42:58.42 CS.seek 0ml @13:42:58.50 CSV.dial :air @13:42:59.19
CTV.dial :air @13:42:59.28 delay 15 seconds @13:42:59.30 CS.seek 10ml @13:43:14.31
SSV.dial :spit,avoiding: :exhaust @13:43:15.65 CTV.dial CSV,avoiding: :intake @13:43:15.70
SS.reconfigure :exhale @13:43:15.78 SS.seek 0ml @13:43:16.16 SSV.dial CBV,avoiding: :exhaust @13:43:23.81 SSV.dial between CBV and plug @13:43:24.30 CBV.dial between SSV and waste2 @13:43:24.70 CSV.dial between air and CTV @13:43:25.02 CS.seek 7.495ml @13:43:31.54 CTV.dial CSV,avoiding: :air @13:43:31.56
CSV.dial CTV @13:43:31.91 CS.reconfigure :pressurize @13:43:31.96 CS.seek 0ml @13:43:45.26 BubblePoint: Intake @24.1psia, Outlet @3.3psia @13:43:45.27 CBV.dial SSV @13:43:45.60 SS.reconfigure :default @13:43:45.65 SS.seek 10ml @13:43:51.23 delay 15 seconds @13:44:06.36 Evacuated: Intake @23.8psia, Outlet @23.6psia @13:44:06.37 SSV.dial :spit,avoiding: :exhaust @13:44:07.41 SS.reconfigure :exhale @13:44:07.46 SS.seek 0ml @13:44:12.57 SS.reconfigure :fast @13:44:12.62 SS.seek 5ml @13:44:15.49 SSV.dial CBV,avoiding: :exhaust @13:44:15.51
CBV.dial SSV @13:44:16.84 CTV.dial :air,avoiding: :intake @13:44:18.17 CSV.dial CTV @13:44:18.21
SS.seek 10ml @13:44:19.34 CSV.dial :air @13:44:19.80 CS.reconfigure :fast @13:44:19.85 CS.seek 0ml @13:44:20.84
SSV.dial :spit,avoiding: :exhaust @13:44:22.18 SS.reconfigure :exhale @13:44:22.22 SS.seek 0ml @13:44:26.50 CS.stop @13:44:27.18
SS.reconfigure :fast @13:44:27.23 SS.seek 5ml @13:44:30.08 SSV.dial CBV,avoiding: :exhaust @13:44:30.10
CBV.dial SSV @13:44:32.43 SSV.dial :spit,avoiding: :exhaust @13:44:33.75 SS.reconfigure :default @13:44:34.23 SS.seek 0ml @13:44:37.50 SS.stop @13:44:37.54 Lysing: Adding 2.0ml AqMeOH @13:44:37.56 CBV.dial between CSV and waste1,avoiding: :DBV @13:44:39.06 CTV.dial CSV @13:44:40.37 CSV.dial CSR @13:44:41.17 Pulling 1ml of CSR.AqMeOH CSR.select :AqMeOH @13:44:41.29 CS.reconfigure :default @13:44:41.33 CS.seek 1ml @13:44:44.75 delay 5 seconds @13:44:49.77 CSR.select :air @13:44:49.94 CS.seek 6ml @13:45:03.59 delay 2 seconds @13:45:05.61 CSV.dial :waste2 @13:45:06.07 CS.reconfigure :fast @13:45:06.11 CS.seek 0ml @13:45:20.40 CSV.dial CSR @13:45:20.98 Pulling 2ml of CSR.AqMeOH @13:45:20.99 CSR.select :AqMeOH @13:45:21.10 CS.reconfigure :default @13:45:21.15 CS.seek 2ml @13:45:27.15 delay 5 seconds @13:45:32.17 CSR.select :air @13:45:32.34 CS.seek 5ml @13:45:40.84 delay 2 seconds @13:45:42.86 CSV.dial CTV @13:45:43.32 CS.seek 1ml @13:45:54.48 Before lysing Collection Puck Heater at 13C/31.2psia @13:45:54.50 Heating Puck.from 5, :sh1 in Collection Clamp to 70C for 10:00 @13:45:54.52 CH.seek 70C,8:00 @13:48:06.21 CH.hold 10:00,70C @13:48:06.41 Begun lysing Collection Puck Heater at 68.1C/37.9psia @13:58:06.41 Ended lysing Collection Puck Heater at 70C/36.9psia @13:58:06.43 CS.seek 0ml @13:58:10.05 CTV.dial between spare and air,avoiding: :intake @13:58:11.08 CSV.dial :air @13:58:11.55 CS.seek 5ml @13:58:25.14 CSV.dial CTV @13:58:25.60 delay 2 seconds @13:58:27.61 CSV.dial :air @13:58:28.06 delay 2 seconds @13:58:30.08 CSV.dial CTV @13:58:30.99 delay 2 seconds @13:58:33.00 CSV.dial :air @13:58:33.46 delay 2 seconds @13:58:35.48 CS.seek 0ml @13:58:49.16 Puck @82.5psia after final lysis pressurization @13:58:49.18 Recovering Lysate into Collection syringe @13:58:49.19 CSV.dial CBV @13:58:50.20 CBV.dial CSV @13:58:50.58 CS.seek 5ml @13:59:04.17 delay 5 seconds @13:59:09.19 CTV.dial :air @13:59:09.74 CS.seek 7.001ml @13:59:15.64 delay 5 seconds @13:59:20.66 CSV.dial :air2 @13:59:21.13 CS.seek 10ml @13:59:29.58 CSV.dial between air and CTV,avoiding: CBV @13:59:30.74 Lysing Complete @13:59:30.81 Storing sh1 puck in Collection Clamp to tube 4 @13:59:30.99 SC.reconfigure :tight @13:59:31.03 SC.seek 4 @13:59:34.59 SC.configure :limp @13:59:34.81 Elbow.reconfigure :tight @13:59:34.86 Elbow.seek 4 @13:59:36.57 Forearm.reconfigure :tight @13:59:36.62 Forearm.seek 4 + 450 counts @13:59:37.70 SE.reconfigure :pick @13:59:37.76 SE.seek :beamBreak @13:59:40.07 SE.configure :default @13:59:40.23 Elbow.reconfigure :limp @13:59:40.28 Forearm.reconfigure :loose @13:59:40.33 Forearm.seek 4 @13:59:40.61 Elbow.hold @13:59:40.67 Elbow.reconfigure :tight @13:59:40.78 SE.seek 12.15 Pucks below Top Plate @13:59:41.23 Forearm.force -20 @13:59:41.27 Forearm.seek 4 + 303 counts @13:59:41.98 Forearm.reconfigure :tight @13:59:42.04 Forearm.seek 4 + 450 counts @13:59:42.24 SE.seek 13.15 Pucks below Top Plate @13:59:42.79 Forearm.reconfigure :loose @13:59:42.84 Forearm.seek :retract @13:59:43.78 Elbow.seek CC @13:59:45.46 Elbow.reconfigure :loose @13:59:45.56 Forearm.seek CC @13:59:46.58 Hand.close @13:59:47.24 CC.seek :open @13:59:51.96 Forearm.seek :retract @13:59:52.92 Elbow.reconfigure :tight @13:59:52.97 Elbow.seek 4 @13:59:54.72 Elbow.reconfigure :limp @13:59:54.77 Forearm.seek 4 @13:59:55.70 Elbow.hold @13:59:55.77 Elbow.reconfigure :tight @13:59:55.82 Hand.open @13:59:56.60 Forearm.force -20 @13:59:56.65 Forearm.seek 4 + 313 counts @13:59:57.04 Forearm.reconfigure :tight @13:59:57.09 Forearm.seek 4 + 450 counts @13:59:57.35 SE.seek :down @13:59:58.48
Forearm.reconfigure :loose @13:59:58.53 Forearm.seek :retract @13:59:59.15 SE.stop @13:59:59.46
Tranferring DA @13:59:59.47 Beginning samplePassC @13:59:59.48 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @13:59:59.67 Elbow.seek FlushPuck::Garage @14:00:01.94 Elbow.reconfigure :loose @14:00:02.04 Forearm.seek :garage @14:00:03.00 Hand.close @14:00:03.73 Forearm.seek :retract @14:00:04.75 Elbow.reconfigure :tight @14:00:04.80 Elbow.seek CC @14:00:07.78 Elbow.reconfigure :loose @14:00:07.88 Forearm.reconfigure :tight @14:00:07.93 Forearm.seek CC @14:00:09.24 CC.seek :closed @14:00:13.78 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.41 @14:00:13.84 Hand.open @14:00:14.54 Forearm.reconfigure :loose @14:00:14.59 Forearm.seek :retract @14:00:15.54 PSV.dial PSR @14:00:16.93 Pulling 1.5ml of PSR.flush @14:00:16.94 PSR.select :flush @14:00:17.07 PS.seek 1.5ml @14:00:33.20 delay 5 seconds @14:00:38.22 PSV.dial :waste1 @14:00:38.70 PS.seek 0ml @14:00:54.86 PSV.dial PSR @14:00:55.33 PSR.select :air @14:00:55.45 PS.seek 2.5ml @14:01:21.90 PRV.dial CBV,avoiding: :ab2 @14:01:21.94
CBV.dial PRV @14:01:24.13 PSV.dial :mixloop @14:01:25.18 CTV.dial :air,avoiding: :intake @14:01:25.34 PTV.dial :PRVmixing @14:01:26.68 PS.seek 0ml @14:01:52.98 PSV.dial :air @14:01:53.46 PS.seek 2.5ml @14:02:19.80 PSV.dial :mixloop @14:02:20.29 PS.seek 0ml @14:02:46.58 PSV.dial :air @14:02:47.05 PS.seek 2.5ml @14:03:13.42 PSV.dial :mixloop @14:03:13.90 PS.seek 0ml @14:03:40.45 Garaging FlushPuck in Collection Clamp @14:03:40.52 Elbow.reconfigure :tight @14:03:40.57 Elbow.seek CC @14:03:40.90 Elbow.reconfigure :loose @14:03:41.00 Forearm.seek CC @14:03:42.04 Hand.close @14:03:42.61 CC.seek :open @14:03:47.22 Forearm.seek :retract @14:03:48.20 Elbow.reconfigure :tight @14:03:48.25 Elbow.seek :garage @14:03:51.23 Elbow.reconfigure :loose @14:03:51.33 Forearm.reconfigure :tight @14:03:51.38 Forearm.seek :garage @14:03:52.42 Hand.open @14:03:53.04 Forearm.reconfigure :loose @14:03:53.09 Forearm.seek :retract @14:03:54.07 PSV.dial :mixloop @14:03:54.15 PTV.dial :PRVmixing @14:03:54.24 PRV.dial CBV @14:03:54.27
CBV.dial PRV @14:03:54.41 CTV.dial :air,avoiding: :intake @14:03:54.43 Loading lyfil puck from tube 5 into Collection Clamp @14:03:55.11 SC.reconfigure :tight @14:03:55.16 SC.seek 5 @14:03:58.73 SC.configure :limp @14:03:58.95 Elbow.reconfigure :tight @14:03:59.00 Elbow.seek 5 @14:04:00.96 Forearm.reconfigure :tight @14:04:01.01 Forearm.seek 5 + 450 counts @14:04:02.04 SE.reconfigure :pick @14:04:02.11 SE.seek :beamBreak @14:04:03.96 SE.configure :default @14:04:04.13 Elbow.seek 5 @14:04:04.31 Forearm.seek 5 + 300 counts @14:04:04.59 Forearm.reconfigure :loose @14:04:04.64 Hand.close @14:04:05.25 SE.seek :down @14:04:05.45
Forearm.seek :retract @14:04:06.45 Elbow.seek CC @14:04:06.83 SE.stop @14:04:08.05
Elbow.reconfigure :loose @14:04:08.15 Forearm.reconfigure :tight @14:04:08.20 Forearm.seek CC @14:04:09.42 CC.seek :closed @14:04:13.96 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.18 @14:04:13.97 Hand.open @14:04:14.68 Forearm.reconfigure :loose @14:04:14.72 Forearm.seek :retract @14:04:15.69 CTV.dial CSV,avoiding: :intake @14:04:17.03 CSV.dial CTV @14:04:17.36 PS.seek 2.5ml @14:04:43.96 CS.seek 4.5ml @14:04:58.92 delay 8 seconds @14:05:06.94 PSV.dial :air @14:05:07.53 delay 2 seconds @14:05:09.55 PSV.dial :waste2 @14:05:10.42 PS.seek 0ml @14:05:36.76 CBV.dial between PRV and waste1 @14:05:37.09 CSV.dial :air @14:05:37.55 CS.seek 10ml @14:05:52.51 CSV.dial CTV @14:05:52.97 CS.seek 0ml @14:06:19.63 CBV.dial :waste1 @14:06:19.95 delay 5 seconds @14:06:24.97 CTV.dial :air,avoiding: :intake @14:06:26.37 Storing lyfil puck in Collection Clamp to tube 4 @14:06:26.54 SC.reconfigure :tight @14:06:26.59 SC.seek 4 @14:06:30.17 SC.configure :limp @14:06:30.39 Elbow.reconfigure :tight @14:06:30.44 Elbow.seek 4 @14:06:32.22 Forearm.reconfigure :tight @14:06:32.27 Forearm.seek 4 + 450 counts @14:06:33.38 SE.reconfigure :pick @14:06:33.44 SE.seek :beamBreak @14:06:35.68 SE.configure :default @14:06:35.85 Elbow.reconfigure :limp @14:06:36.34 Forearm.reconfigure :loose @14:06:36.39 Forearm.seek 4 @14:06:36.59 Elbow.hold @14:06:36.65 Elbow.reconfigure :tight @14:06:36.76 SE.seek 13.13 Pucks below Top Plate @14:06:37.36 Forearm.force -20 @14:06:37.40 Forearm.seek 4 + 307 counts @14:06:37.82 Forearm.reconfigure :tight @14:06:37.87 Forearm.seek 4 + 450 counts @14:06:38.06 SE.seek 14.13 Pucks below Top Plate @14:06:38.60 Forearm.reconfigure :loose @14:06:38.65 Forearm.seek :retract @14:06:39.57 Elbow.seek CC @14:06:41.24 Elbow.reconfigure :loose @14:06:41.34 Forearm.seek CC @14:06:42.32 Hand.close @14:06:42.98 CC.seek :open @14:06:47.72 Forearm.seek :retract @14:06:48.72 Elbow.reconfigure :tight @14:06:48.76 Elbow.seek 4 @14:06:50.52 Elbow.reconfigure :limp @14:06:50.57 Forearm.seek 4 @14:06:51.54 Elbow.hold @14:06:51.61 Elbow.reconfigure :tight @14:06:51.65 Hand.open @14:06:52.35 Forearm.force -20 @14:06:52.39 Forearm.seek 4 + 320 counts @14:06:53.23 Forearm.reconfigure :tight @14:06:53.28 Forearm.seek 4 + 450 counts @14:06:53.52 SE.seek :down @14:06:54.66
Forearm.reconfigure :loose @14:06:54.71 Forearm.seek :retract @14:06:55.27 SE.stop @14:06:55.62
PSV.dial :air @14:06:56.49 PS.seek 2.5ml @14:07:22.82 delay 1 second @14:07:23.84 PSV.dial :mixloop @14:07:24.31 delay 1 second @14:07:25.32 PSV.dial :air @14:07:25.79 delay 1 second @14:07:26.81 PSV.dial :mixloop @14:07:27.28 delay 1 second @14:07:28.29 PSV.dial :air @14:07:28.76 delay 1 second @14:07:29.78 PS.seek 0ml @14:07:56.17 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @14:07:56.35 Elbow.seek FlushPuck::Garage @14:07:58.21 Elbow.reconfigure :loose @14:07:58.31 Forearm.seek :garage @14:07:59.32 Hand.close @14:08:00.10 Forearm.seek :retract @14:08:01.15 Elbow.reconfigure :tight @14:08:01.21 Elbow.seek PC @14:08:02.49 Elbow.reconfigure :loose @14:08:02.59 Forearm.reconfigure :tight @14:08:02.64 Forearm.seek PC @14:08:03.90 PC.seek :closed @14:08:08.56 Processing Clamp onSpring/offSpring for 16/26 samples, on/off torque 1.63 @14:08:08.64 Hand.open @14:08:09.29 Forearm.reconfigure :loose @14:08:09.34 Forearm.seek :retract @14:08:10.27 PRV.dial :air @14:08:10.73 PSV.dial :mixloop @14:08:11.27 PS.seek 0.3ml @14:08:15.10 PSV.dial PSR @14:08:16.28 Pulling 0.4ml of PSR.air @14:08:16.29 PSR.select :air @14:08:16.31 PS.seek 0.7ml @14:08:21.20 delay 3 seconds @14:08:24.21 PSR.select :air @14:08:24.24 PS.seek 2.5ml @14:08:43.41 delay 3 seconds @14:08:46.43 PSV.dial :mixloop @14:08:47.59 PTV.dial :mixing @14:08:48.35 PBV.dial :waste2 @14:08:49.33 PS.seek 0ml @14:09:15.67 Garaging FlushPuck in Processing Clamp @14:09:15.74 Elbow.reconfigure :tight @14:09:15.79 Elbow.seek PC @14:09:15.91 Elbow.reconfigure :loose @14:09:16.01 Forearm.seek PC @14:09:16.98 Hand.close @14:09:17.60 PC.seek :open @14:09:22.31 Forearm.seek :retract @14:09:23.28 Elbow.reconfigure :tight @14:09:23.32 Elbow.seek :garage @14:09:24.69 Elbow.reconfigure :loose @14:09:24.79 Forearm.reconfigure :tight @14:09:24.84 Forearm.seek :garage @14:09:25.85 Hand.open @14:09:26.96 Forearm.reconfigure :loose @14:09:27.02 Forearm.seek :retract @14:09:28.01 Loading da puck from tube 5 into Processing Clamp @14:09:28.25 SC.reconfigure :tight @14:09:28.30 SC.seek 5 @14:09:31.85 SC.configure :limp @14:09:32.06 Elbow.reconfigure :tight @14:09:32.11 Elbow.seek 5 @14:09:33.98 Forearm.reconfigure :tight @14:09:34.03 Forearm.seek 5 + 450 counts @14:09:35.08 SE.reconfigure :pick @14:09:35.14 SE.seek :beamBreak @14:09:37.15 SE.configure :default @14:09:37.31 Elbow.seek 5 @14:09:37.49 Forearm.seek 5 + 300 counts @14:09:37.69 Forearm.reconfigure :loose @14:09:37.74 Hand.close @14:09:38.37 SE.seek :down @14:09:38.56
Forearm.seek :retract @14:09:39.53 Elbow.seek PC @14:09:40.02 SE.stop @14:09:42.13
Elbow.reconfigure :loose @14:09:42.23 Forearm.reconfigure :tight @14:09:42.28 Forearm.seek PC @14:09:43.52 PC.seek :closed @14:09:48.18 Processing Clamp onSpring/offSpring for 16/26 samples, on/off torque 1.64 @14:09:48.19 Hand.open @14:09:48.91 Forearm.reconfigure :loose @14:09:48.96 Forearm.seek :retract @14:09:49.92 Heating Puck.from 5, :da in Processing Clamp to 28C for 2:00:00 @14:09:49.94 PH.seek 28C,5:00 @14:10:33.30 PH.hold 2:00:00,28C @14:10:33.36 delay 1:30 @14:12:03.40 PSR.select :air @14:12:03.42 PSV.dial PSR @14:12:04.46 Pulling 0.5ml of PSR.wash @14:12:04.47 PSR.select :wash @14:12:05.04 PS.seek 0.5ml @14:12:11.04 delay 5 seconds @14:12:16.06 PSR.select :air @14:12:16.20 PS.seek 2ml @14:12:32.32 PTV.dial :puck @14:12:33.07 PSV.dial PTV @14:12:33.83 PBV.dial :waste1 @14:12:35.20 PS.seek 1.5ml @14:12:41.16 delay 3 seconds @14:12:44.18 PBV.dial between PSV and waste1 @14:12:44.54 PS.seek 0ml @14:13:00.69 delay 5:00 @14:18:00.71 recoverPSRreagent08 @14:18:00.72 PTV.dial :puck @14:18:00.80 PBV.dial :waste1 @14:18:01.17 delay 30 seconds @14:18:31.20 PBV.dial between PSV and waste1 @14:18:31.58 PSR.select :air @14:18:31.59 PSV.dial PSR @14:18:32.36 PS.reconfigure :fast @14:18:32.41 PS.seek 2.5ml @14:18:56.25 PSV.dial PTV @14:18:57.01 delay 5 seconds @14:19:02.04 PSV.dial PSR @14:19:02.80 delay 5 seconds @14:19:07.81 PSV.dial PTV @14:19:08.59 delay 5 seconds @14:19:13.61 PSV.dial :air @14:19:14.49 PS.seek 0ml @14:19:37.62 PTV.dial :PRVmixing,via: PRV @14:19:38.97 PSV.dial :mixloop @14:19:39.42 PRV.dial :ab1 @14:19:40.17 PS.reconfigure :slow @14:19:40.22 PS.seek 0.15ml @14:19:43.78 delay 2 seconds @14:19:45.80 CBV.dial PRV @14:19:45.82
PRV.dial CBV @14:19:46.40 PS.seek 0.35ml @14:19:51.03 delay 2 seconds @14:19:53.05 CBV.dial between PRV and PSV @14:19:53.36 PRV.dial :air @14:19:53.82 PS.seek 0.9ml @14:20:05.59 PRV.dial :waste2 @14:20:06.35 PS.seek 0.5ml @14:20:15.06 addPMLReagent08 @14:20:15.07 PSV.dial :air @14:20:15.55 PS.reconfigure :default @14:20:15.60 PS.seek 2ml @14:20:31.71 PSV.dial :PML @14:20:32.18 PTV.dial :mixing @14:20:32.99 PBV.dial :waste1 @14:20:33.43 PS.seek 1.85ml @14:20:35.87 PBV.dial between PSV and waste1 @14:20:36.24 PS.seek 0ml @14:20:55.96 delay 2 seconds @14:20:57.98 Beginning samplePassC @14:20:58.00 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:20:58.18 Elbow.reconfigure :tight @14:20:58.23 Elbow.seek FlushPuck::Garage @14:20:59.63 Elbow.reconfigure :loose @14:20:59.73 Forearm.seek :garage @14:21:00.70 Hand.close @14:21:01.47 Forearm.seek :retract @14:21:02.47 Elbow.reconfigure :tight @14:21:02.52 Elbow.seek CC @14:21:05.59 Elbow.reconfigure :loose @14:21:05.69 Forearm.reconfigure :tight @14:21:05.74 Forearm.seek CC @14:21:07.02 CC.seek :closed @14:21:11.54 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.52 @14:21:11.60 Hand.open @14:21:12.28 Forearm.reconfigure :loose @14:21:12.33 Forearm.seek :retract @14:21:13.29 PSV.dial PSR @14:21:14.35 Pulling 1.5ml of PSR.flush @14:21:14.36 PSR.select :flush @14:21:14.93 PS.seek 1.5ml @14:21:31.12 delay 5 seconds @14:21:36.14 PSV.dial :waste1 @14:21:36.61 PS.seek 0ml @14:21:52.74 PSV.dial PSR @14:21:53.21 PSR.select :air @14:21:53.33 PS.seek 2.5ml @14:22:19.75 PRV.dial CBV,avoiding: :ab2 @14:22:19.77
CBV.dial PRV @14:22:21.42 PSV.dial :mixloop @14:22:22.47 CTV.dial :air,avoiding: :intake @14:22:22.54 PTV.dial :PRVmixing @14:22:23.31 PS.seek 0ml @14:22:49.84 PSV.dial :air @14:22:50.31 PS.seek 2.5ml @14:23:16.58 PSV.dial :mixloop @14:23:17.06 PS.seek 0ml @14:23:43.37 PSV.dial :air @14:23:43.84 PS.seek 2.5ml @14:24:10.21 PSV.dial :mixloop @14:24:10.69 PS.seek 0ml @14:24:37.02 Garaging FlushPuck in Collection Clamp @14:24:37.09 Elbow.reconfigure :tight @14:24:37.14 Elbow.seek CC @14:24:37.26 Elbow.reconfigure :loose @14:24:37.36 Forearm.seek CC @14:24:38.34 Hand.close @14:24:38.97 CC.seek :open @14:24:43.58 Forearm.seek :retract @14:24:44.53 Elbow.reconfigure :tight @14:24:44.58 Elbow.seek :garage @14:24:47.66 Elbow.reconfigure :loose @14:24:47.76 Forearm.reconfigure :tight @14:24:47.81 Forearm.seek :garage @14:24:48.83 Hand.open @14:24:49.53 Forearm.reconfigure :loose @14:24:49.99 Forearm.seek :retract @14:24:51.00 delayUntil "14:28:57.97" @14:24:51.03 Whole Cell Archive sampling 1000ml @14:24:51.05 Loading wcr puck from tube 5 into Collection Clamp @14:24:51.54 Elbow.reconfigure :tight @14:24:51.59 Elbow.seek 5 @14:24:53.51 Forearm.reconfigure :tight @14:24:53.56 Forearm.seek 5 + 450 counts @14:24:54.59 SE.reconfigure :pick @14:24:54.66 SE.seek :beamBreak @14:24:56.70 SE.configure :default @14:24:56.87 Elbow.seek 5 @14:24:57.07 Forearm.seek 5 + 300 counts @14:24:57.35 Forearm.reconfigure :loose @14:24:57.40 Hand.close @14:24:57.98 SE.seek :down @14:24:58.18 Forearm.seek :retract @14:24:59.12 Elbow.seek CC @14:24:59.71 SE.stop @14:25:00.79 Elbow.reconfigure :loose @14:25:00.89 Forearm.reconfigure :tight @14:25:00.95 Forearm.seek CC @14:25:02.63 CC.seek :closed @14:25:07.22 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.38 @14:25:07.23 Hand.open @14:25:07.90 Forearm.reconfigure :loose @14:25:07.95 Forearm.seek :retract @14:25:08.92 collectSample 1000ml w/bubble point of 35psi @14:25:08.94 Priming 7ml @14:25:08.95 Intake.select :close @14:25:08.96 Exhaust.select :close @14:25:08.97 SS.hold @14:25:09.18 Calibrating pressure sensors @14:25:09.19 CTV.dial :air @14:25:09.28 SSV.dial :spit,avoiding: :exhaust @14:25:09.36 SS.reconfigure :fast @14:25:09.42 SS.seek 5ml @14:25:12.11 SSV.dial CBV @14:25:12.13 CBV.dial SSV @14:25:13.51 delay 3.8 seconds @14:25:17.45 Sensor limits: Intake max 103.1psia; Outlet max 94.5psia @14:25:17.46 SSV.dial :spit @14:25:18.79 SS.seek 0ml @14:25:21.70 SSV.dial CBV @14:25:23.05 CTV.dial :intake @14:25:23.79 SSV.dial CBV @14:25:23.81 CBV.dial SSV @14:25:23.97 Allowing 40s for 7.0ml of remaining 7.0ml @14:25:23.99 Intake.select :open @14:25:26.16 Intake @14.3psia, vacuum from 8.8 to 11.8psi @14:25:26.18 SS.reconfigure :inhaling 2.20ml/s @14:25:26.23 SS.seek 7ml @14:25:32.98 Stroke completed -- waiting for pressure to equalize ... @14:25:32.99 SS.reconfigure :exhale @14:25:33.04 SS.hold @14:25:47.70 Intake.select :close @14:25:47.86 SSV.dial :exhaust @14:25:48.64 Exhaust.select :open @14:25:48.76 SS.seek 0ml @14:25:53.03 Exhaust.select :close @14:25:53.14 Sampling 1000.0ml @14:25:53.15 Intake.select :close @14:25:53.16 Exhaust.select :close @14:25:53.18 SS.reconfigure :default @14:25:53.22 SS.hold @14:25:53.44 CTV.dial :intake @14:25:53.51 SSV.dial CBV @14:25:53.54 CBV.dial SSV @14:25:54.32 Allowing 130s for 25.0ml of remaining 1000.0ml @14:25:54.34 Intake.select :open @14:25:56.51 Intake @15.4psia, vacuum from 9.9 to 12.9psi @14:25:56.53 SS.reconfigure :inhaling 2.20ml/s @14:25:56.58 SS.seek 25ml @14:26:04.80 Vacuum too high -- waiting up to 40 seconds @14:26:04.81 SS.reconfigure :exhale @14:26:04.87 SS.hold @14:26:13.81 Intake @19.1psia, vacuum from 10.0 to 13.0psi @14:26:13.83 SS.reconfigure :inhaling 1.76ml/s @14:26:13.88 SS.seek 25ml @14:26:15.97 Vacuum too high -- waiting up to 40 seconds @14:26:15.99 SS.reconfigure :exhale @14:26:16.05 SS.hold @14:26:17.89 Intake @18.6psia, vacuum from 10.0 to 13.0psi @14:26:17.91 SS.reconfigure :inhaling 1.41ml/s @14:26:17.95 SS.seek 25ml @14:26:20.57 Vacuum too high -- waiting up to 40 seconds @14:26:20.58 SS.reconfigure :exhale @14:26:20.64 SS.hold @14:26:22.81 Intake @19.1psia, vacuum from 10.0 to 13.0psi @14:26:22.83 SS.reconfigure :inhaling 1.13ml/s @14:26:22.88 SS.seek 25ml @14:26:25.47 Vacuum too high -- waiting up to 40 seconds @14:26:25.49 SS.reconfigure :exhale @14:26:25.55 SS.hold @14:26:27.69 Intake @19.1psia, vacuum from 10.0 to 13.0psi @14:26:27.71 SS.reconfigure :inhaling 0.91ml/s @14:26:27.76 SS.seek 25ml @14:26:31.89 Vacuum too high -- waiting up to 40 seconds @14:26:31.90 SS.reconfigure :exhale @14:26:31.96 SS.hold @14:26:34.09 Intake @19.3psia, vacuum from 10.0 to 13.0psi @14:26:34.11 SS.reconfigure :inhaling 0.73ml/s @14:26:34.16 SS.seek 25ml @14:26:37.19 Stroke completed -- waiting for pressure to equalize ... @14:26:37.20 SS.reconfigure :exhale @14:26:37.25 SS.hold @14:27:04.12 Intake.select :close @14:27:04.28 SSV.dial :exhaust @14:27:05.05 Exhaust.select :open @14:27:05.17 SS.seek 0ml @14:27:14.36 Exhaust.select :close @14:27:14.49 SSV.dial CBV @14:27:14.52 CBV.dial SSV @14:27:15.31 Allowing 130s for 25.0ml of remaining 975.0ml @14:27:15.32 Intake.select :open @14:27:17.50 Intake @15.8psia, vacuum from 10.0 to 13.0psi @14:27:17.52 SS.reconfigure :inhaling 0.73ml/s @14:27:17.57 SS.seek 25ml @14:27:54.04 Stroke completed -- waiting for pressure to equalize ... @14:27:54.05 SS.reconfigure :exhale @14:27:54.10 SS.hold @14:28:26.81 Intake.select :close @14:28:26.97 SSV.dial :exhaust @14:28:27.77 Exhaust.select :open @14:28:27.89 SS.seek 0ml @14:28:36.90 Exhaust.select :close @14:28:37.01 SSV.dial CBV @14:28:37.04 CBV.dial SSV @14:28:37.82 Allowing 130s for 25.0ml of remaining 950.0ml @14:28:37.84 Intake.select :open @14:28:40.00 Intake @15.8psia, vacuum from 10.0 to 13.0psi @14:28:40.02 SS.reconfigure :inhaling 0.73ml/s @14:28:40.07 SS.seek 25ml @14:28:57.99 recoverPRVreagent08 @14:28:58.00 PTV.dial :mixing @14:28:58.77 PBV.dial :waste1 @14:28:59.14 delay 30 seconds @14:29:15.99 Stroke completed -- waiting for pressure to equalize ... @14:29:16.00 SS.reconfigure :exhale @14:29:16.05 SS.hold @14:29:29.16 PBV.dial between PSV and waste1 @14:29:29.54 PSV.dial :air @14:29:30.01 PS.reconfigure :fast @14:29:30.06 PS.seek 2.5ml @14:29:44.71 Intake.select :close @14:29:44.87 SSV.dial :exhaust @14:29:45.66 Exhaust.select :open @14:29:45.78 SS.seek 0ml @14:29:54.01 PSV.dial :mixloop @14:29:54.50 delay 2 seconds @14:29:54.77 Exhaust.select :close @14:29:54.89 SSV.dial CBV @14:29:54.91 CBV.dial SSV @14:29:55.71 Allowing 130s for 25.0ml of remaining 925.0ml @14:29:55.72 Intake.select :open @14:29:56.52 PSV.dial :air @14:29:56.99 delay 2 seconds @14:29:57.89 Intake @15.9psia, vacuum from 10.0 to 13.0psi @14:29:57.91 SS.reconfigure :inhaling 0.73ml/s @14:29:57.96 SS.seek 25ml @14:29:59.00 PSV.dial :mixloop @14:29:59.92 delay 2 seconds @14:30:01.94 PSV.dial :air @14:30:02.54 PS.seek 0ml @14:30:26.05 washCycleDA #1 of 2 @14:30:26.06 PSR.select :air @14:30:26.08 PSV.dial PSR @14:30:27.44 Pulling 0.5ml of PSR.wash @14:30:27.45 PSR.select :wash @14:30:27.57 PS.reconfigure :default @14:30:27.62 PS.seek 0.5ml @14:30:33.52 delay 5 seconds @14:30:33.80 Stroke completed -- waiting for pressure to equalize ... @14:30:33.82 SS.reconfigure :exhale @14:30:33.86 SS.hold @14:30:38.53 PSR.select :air @14:30:38.66 PS.seek 2ml @14:30:54.86 PTV.dial :puck @14:30:55.61 PSV.dial PTV @14:30:56.37 PBV.dial :waste1 @14:30:56.81 PS.seek 1.5ml @14:31:00.64 Intake.select :close @14:31:00.80 SSV.dial :exhaust @14:31:01.57 Exhaust.select :open @14:31:01.69 SS.seek 0ml @14:31:02.76 delay 3 seconds @14:31:05.78 PBV.dial between PSV and waste1 @14:31:06.18 PS.seek 0ml @14:31:10.46 Exhaust.select :close @14:31:10.57 SSV.dial CBV @14:31:10.60 CBV.dial SSV @14:31:11.40 Allowing 130s for 25.0ml of remaining 900.0ml @14:31:11.41 Intake.select :open @14:31:13.58 Intake @15.9psia, vacuum from 10.0 to 13.0psi @14:31:13.60 SS.reconfigure :inhaling 0.73ml/s @14:31:13.65 SS.seek 25ml @14:31:22.49 delay 2:00 @14:31:50.10 Stroke completed -- waiting for pressure to equalize ... @14:31:50.11 SS.reconfigure :exhale @14:31:50.16 SS.hold @14:32:14.59 Intake.select :close @14:32:14.85 SSV.dial :exhaust @14:32:15.61 Exhaust.select :open @14:32:15.74 SS.seek 0ml @14:32:24.53 Exhaust.select :close @14:32:24.64 SSV.dial CBV @14:32:24.68 CBV.dial SSV @14:32:25.44 Allowing 130s for 25.0ml of remaining 875.0ml @14:32:25.46 Intake.select :open @14:32:27.62 Intake @16.0psia, vacuum from 10.0 to 13.0psi @14:32:27.64 SS.reconfigure :inhaling 0.73ml/s @14:32:27.69 SS.seek 25ml @14:33:04.13 Stroke completed -- waiting for pressure to equalize ... @14:33:04.14 SS.reconfigure :exhale @14:33:04.19 SS.hold @14:33:22.50 recoverPSRreagent08 @14:33:22.52 PTV.dial :puck @14:33:22.53 PBV.dial :waste1 @14:33:22.90 delay 30 seconds @14:33:26.94 Intake.select :close @14:33:27.10 SSV.dial :exhaust @14:33:27.86 Exhaust.select :open @14:33:27.98 SS.seek 0ml @14:33:36.81 Exhaust.select :close @14:33:36.92 SSV.dial CBV @14:33:36.97 CBV.dial SSV @14:33:37.71 Allowing 130s for 25.0ml of remaining 850.0ml @14:33:37.72 Intake.select :open @14:33:39.89 Intake @15.8psia, vacuum from 10.0 to 13.0psi @14:33:39.91 SS.reconfigure :inhaling 0.73ml/s @14:33:39.96 SS.seek 25ml @14:33:52.93 PBV.dial between PSV and waste1 @14:33:53.31 PSR.select :air @14:33:53.33 PSV.dial PSR @14:33:54.10 PS.reconfigure :fast @14:33:54.15 PS.seek 2.5ml @14:34:16.64 Stroke completed -- waiting for pressure to equalize ... @14:34:16.65 SS.reconfigure :exhale @14:34:16.75 SS.hold @14:34:18.22 PSV.dial PTV @14:34:19.03 delay 5 seconds @14:34:24.04 PSV.dial PSR @14:34:24.81 delay 5 seconds @14:34:29.82 PSV.dial PTV @14:34:30.59 delay 5 seconds @14:34:35.61 PSV.dial :air @14:34:36.46 PS.seek 0ml @14:34:37.14 Intake.select :close @14:34:37.30 SSV.dial :exhaust @14:34:38.09 Exhaust.select :open @14:34:38.21 SS.seek 0ml @14:34:47.31 Exhaust.select :close @14:34:47.42 SSV.dial CBV @14:34:47.45 CBV.dial SSV @14:34:48.24 Allowing 130s for 25.0ml of remaining 825.0ml @14:34:48.25 Intake.select :open @14:34:50.42 Intake @15.9psia, vacuum from 10.0 to 13.0psi @14:34:50.44 SS.reconfigure :inhaling 0.73ml/s @14:34:50.49 SS.seek 25ml @14:35:00.17 washCycleDA #2 of 2 @14:35:00.18 PSR.select :air @14:35:00.20 PSV.dial PSR @14:35:01.54 Pulling 0.5ml of PSR.wash @14:35:01.55 PSR.select :wash @14:35:01.67 PS.reconfigure :default @14:35:01.72 PS.seek 0.5ml @14:35:07.70 delay 5 seconds @14:35:12.72 PSR.select :air @14:35:12.84 PS.seek 2ml @14:35:26.48 Stroke completed -- waiting for pressure to equalize ... @14:35:26.50 SS.reconfigure :exhale @14:35:26.55 SS.hold @14:35:29.00 PTV.dial :puck @14:35:29.03 PSV.dial PTV @14:35:29.81 PBV.dial :waste1 @14:35:30.24 PS.seek 1.5ml @14:35:36.23 delay 3 seconds @14:35:39.31 PBV.dial between PSV and waste1 @14:35:39.70 PS.seek 0ml @14:35:49.10 Intake.select :close @14:35:49.26 SSV.dial :exhaust @14:35:50.03 Exhaust.select :open @14:35:50.15 SS.seek 0ml @14:35:55.89 delay 2:00 @14:35:59.00 Exhaust.select :close @14:35:59.12 SSV.dial CBV @14:35:59.14 CBV.dial SSV @14:35:59.94 Allowing 130s for 25.0ml of remaining 800.0ml @14:35:59.95 Intake.select :open @14:36:02.12 Intake @15.8psia, vacuum from 10.0 to 13.0psi @14:36:02.14 SS.reconfigure :inhaling 0.73ml/s @14:36:02.19 SS.seek 25ml @14:36:38.56 Stroke completed -- waiting for pressure to equalize ... @14:36:38.57 SS.reconfigure :exhale @14:36:38.62 SS.hold @14:36:57.23 Intake.select :close @14:36:57.39 SSV.dial :exhaust @14:36:58.16 Exhaust.select :open @14:36:58.28 SS.seek 0ml @14:37:07.09 Exhaust.select :close @14:37:07.20 SSV.dial CBV @14:37:07.23 CBV.dial SSV @14:37:08.05 Allowing 130s for 25.0ml of remaining 775.0ml @14:37:08.06 Intake.select :open @14:37:10.23 Intake @15.9psia, vacuum from 10.0 to 13.0psi @14:37:10.25 SS.reconfigure :inhaling 0.73ml/s @14:37:10.30 SS.seek 25ml @14:37:46.82 Stroke completed -- waiting for pressure to equalize ... @14:37:46.84 SS.reconfigure :exhale @14:37:46.89 SS.hold @14:37:55.91 recoverPSRreagent08 @14:37:55.92 PTV.dial :puck @14:37:55.93 PBV.dial :waste1 @14:37:56.31 delay 30 seconds @14:38:05.57 Intake.select :close @14:38:05.73 SSV.dial :exhaust @14:38:06.50 Exhaust.select :open @14:38:06.62 SS.seek 0ml @14:38:15.45 Exhaust.select :close @14:38:15.56 SSV.dial CBV @14:38:15.61 CBV.dial SSV @14:38:16.36 Allowing 130s for 25.0ml of remaining 750.0ml @14:38:16.37 Intake.select :open @14:38:19.00 Intake @16.0psia, vacuum from 10.0 to 13.0psi @14:38:19.02 SS.reconfigure :inhaling 0.73ml/s @14:38:19.07 SS.seek 25ml @14:38:26.33 PBV.dial between PSV and waste1 @14:38:26.69 PSR.select :air @14:38:26.71 PSV.dial PSR @14:38:27.48 PS.reconfigure :fast @14:38:27.53 PS.seek 2.5ml @14:38:51.54 PSV.dial PTV @14:38:52.30 delay 5 seconds @14:38:55.76 Stroke completed -- waiting for pressure to equalize ... @14:38:55.78 SS.reconfigure :exhale @14:38:55.82 SS.hold @14:38:57.32 PSV.dial PSR @14:38:58.08 delay 5 seconds @14:39:03.10 PSV.dial PTV @14:39:03.87 delay 5 seconds @14:39:08.88 PSV.dial :air @14:39:09.78 PS.seek 0ml @14:39:16.27 Intake.select :close @14:39:16.43 SSV.dial :exhaust @14:39:17.20 Exhaust.select :open @14:39:17.32 SS.seek 0ml @14:39:26.12 Exhaust.select :close @14:39:26.24 SSV.dial CBV @14:39:26.26 CBV.dial SSV @14:39:27.07 Allowing 130s for 25.0ml of remaining 725.0ml @14:39:27.09 Intake.select :open @14:39:29.26 Intake @15.9psia, vacuum from 10.0 to 13.0psi @14:39:29.28 SS.reconfigure :inhaling 0.73ml/s @14:39:29.33 SS.seek 25ml @14:39:33.48 getPMLReagent08 @14:39:33.49 PRV.dial :air @14:39:33.94 delay 5 seconds @14:39:38.97 PTV.dial :PRVmixing @14:39:40.29 delay 5 seconds @14:39:45.31 PRV.dial :ab2 @14:39:45.77 PTV.dial :PRVmixing @14:39:45.78 PSV.dial :mixloop @14:39:46.24 PS.reconfigure :default @14:39:46.30 PS.seek 0.3ml @14:39:50.29 delay 2 seconds @14:39:52.30 PRV.dial :air @14:39:52.85 PS.seek 0.55ml @14:39:56.23 addPMLReagent08 @14:39:56.24 PSV.dial :air @14:39:56.71 PS.seek 2ml @14:40:05.74 Stroke completed -- waiting for pressure to equalize ... @14:40:05.76 SS.reconfigure :exhale @14:40:05.81 SS.hold @14:40:12.39 PSV.dial :PML @14:40:12.86 PTV.dial :mixing @14:40:13.63 PBV.dial :waste1 @14:40:14.05 PS.seek 1.85ml @14:40:16.42 PBV.dial between PSV and waste1 @14:40:16.79 PS.seek 0ml @14:40:26.28 Intake.select :close @14:40:26.44 SSV.dial :exhaust @14:40:27.21 Exhaust.select :open @14:40:27.33 SS.seek 0ml @14:40:36.18 Exhaust.select :close @14:40:36.30 SSV.dial CBV @14:40:36.32 CBV.dial SSV @14:40:36.46 delay 2 seconds @14:40:37.12 Allowing 130s for 25.0ml of remaining 700.0ml @14:40:37.14 Intake.select :open @14:40:38.48 delay 8:00 @14:40:39.30 Intake @15.9psia, vacuum from 10.0 to 13.0psi @14:40:39.32 SS.reconfigure :inhaling 0.73ml/s @14:40:39.37 SS.seek 25ml @14:41:15.93 Stroke completed -- waiting for pressure to equalize ... @14:41:15.94 SS.reconfigure :exhale @14:41:16.00 SS.hold @14:41:38.80 Intake.select :close @14:41:38.96 SSV.dial :exhaust @14:41:39.73 Exhaust.select :open @14:41:39.85 SS.seek 0ml @14:41:48.84 Exhaust.select :close @14:41:48.95 SSV.dial CBV @14:41:48.98 CBV.dial SSV @14:41:50.23 Allowing 130s for 25.0ml of remaining 675.0ml @14:41:50.24 Intake.select :open @14:41:52.41 Intake @15.8psia, vacuum from 10.0 to 13.0psi @14:41:52.43 SS.reconfigure :inhaling 0.73ml/s @14:41:52.48 SS.seek 25ml @14:42:28.60 Stroke completed -- waiting for pressure to equalize ... @14:42:28.61 SS.reconfigure :exhale @14:42:28.66 SS.hold @14:42:53.25 Intake.select :close @14:42:53.41 SSV.dial :exhaust @14:42:54.20 Exhaust.select :open @14:42:54.32 SS.seek 0ml @14:43:03.15 Exhaust.select :close @14:43:03.26 SSV.dial CBV @14:43:03.31 CBV.dial SSV @14:43:04.06 Allowing 130s for 25.0ml of remaining 650.0ml @14:43:04.07 Intake.select :open @14:43:06.24 Intake @15.9psia, vacuum from 10.0 to 13.0psi @14:43:06.26 SS.reconfigure :inhaling 0.73ml/s @14:43:06.31 SS.seek 25ml @14:43:33.02 Vacuum too high -- waiting up to 40 seconds @14:43:33.03 SS.reconfigure :exhale @14:43:33.09 SS.hold @14:43:42.88 Intake @17.5psia, vacuum from 10.0 to 13.0psi @14:43:42.90 SS.reconfigure :inhaling 0.59ml/s @14:43:43.42 SS.seek 25ml @14:43:56.27 Stroke completed -- waiting for pressure to equalize ... @14:43:56.28 SS.reconfigure :exhale @14:43:56.33 SS.hold @14:44:12.71 Intake.select :close @14:44:12.87 SSV.dial :exhaust @14:44:13.63 Exhaust.select :open @14:44:13.75 SS.seek 0ml @14:44:22.77 Exhaust.select :close @14:44:22.89 SSV.dial CBV @14:44:22.91 CBV.dial SSV @14:44:23.71 Allowing 130s for 25.0ml of remaining 625.0ml @14:44:24.18 Intake.select :open @14:44:26.36 Intake @15.8psia, vacuum from 10.0 to 13.0psi @14:44:26.38 SS.reconfigure :inhaling 0.59ml/s @14:44:26.43 SS.seek 25ml @14:45:10.85 Stroke completed -- waiting for pressure to equalize ... @14:45:10.86 SS.reconfigure :exhale @14:45:10.91 SS.hold @14:45:33.30 Intake.select :close @14:45:33.91 SSV.dial :exhaust @14:45:34.68 Exhaust.select :open @14:45:34.80 SS.seek 0ml @14:45:43.80 Exhaust.select :close @14:45:43.92 SSV.dial CBV @14:45:43.95 CBV.dial SSV @14:45:44.71 Allowing 130s for 25.0ml of remaining 600.0ml @14:45:44.72 Intake.select :open @14:45:46.89 Intake @15.9psia, vacuum from 10.0 to 13.0psi @14:45:46.91 SS.reconfigure :inhaling 0.59ml/s @14:45:46.96 SS.seek 25ml @14:46:31.45 Stroke completed -- waiting for pressure to equalize ... @14:46:31.47 SS.reconfigure :exhale @14:46:31.52 SS.hold @14:46:54.02 Intake.select :close @14:46:54.18 SSV.dial :exhaust @14:46:54.96 Exhaust.select :open @14:46:55.08 SS.seek 0ml @14:47:03.94 Exhaust.select :close @14:47:04.05 SSV.dial CBV @14:47:04.08 CBV.dial SSV @14:47:04.86 Allowing 130s for 25.0ml of remaining 575.0ml @14:47:04.88 Intake.select :open @14:47:07.04 Intake @16.0psia, vacuum from 10.0 to 13.0psi @14:47:07.06 SS.reconfigure :inhaling 0.59ml/s @14:47:07.11 SS.seek 25ml @14:47:51.75 Stroke completed -- waiting for pressure to equalize ... @14:47:51.76 SS.reconfigure :exhale @14:47:51.81 SS.hold @14:48:16.70 Intake.select :close @14:48:16.86 SSV.dial :exhaust @14:48:17.64 Exhaust.select :open @14:48:17.76 SS.seek 0ml @14:48:26.59 Exhaust.select :close @14:48:26.70 SSV.dial CBV @14:48:26.73 CBV.dial SSV @14:48:27.52 Allowing 130s for 25.0ml of remaining 550.0ml @14:48:27.53 Intake.select :open @14:48:29.70 Intake @15.9psia, vacuum from 10.0 to 13.0psi @14:48:29.72 SS.reconfigure :inhaling 0.59ml/s @14:48:29.77 SS.seek 25ml @14:48:38.51 recoverPRVreagent08 @14:48:38.52 PTV.dial :mixing @14:48:38.59 PBV.dial :waste1 @14:48:38.97 delay 30 seconds @14:49:08.99 PBV.dial between PSV and waste1 @14:49:09.37 PSV.dial :air @14:49:09.97 PS.reconfigure :fast @14:49:10.02 PS.seek 2.5ml @14:49:14.66 Stroke completed -- waiting for pressure to equalize ... @14:49:14.68 SS.reconfigure :exhale @14:49:14.73 SS.hold @14:49:34.18 PSV.dial :mixloop @14:49:34.64 delay 2 seconds @14:49:36.65 PSV.dial :air @14:49:37.12 delay 2 seconds @14:49:39.14 PSV.dial :mixloop @14:49:39.60 delay 2 seconds @14:49:41.49 Intake.select :close @14:49:41.65 SSV.dial :exhaust @14:49:41.71 PSV.dial :air @14:49:42.19 PS.seek 0ml @14:49:42.44 Exhaust.select :open @14:49:42.56 SS.seek 0ml @14:49:51.64 Exhaust.select :close @14:49:51.75 SSV.dial CBV @14:49:52.18 CBV.dial SSV @14:49:53.02 Allowing 130s for 25.0ml of remaining 525.0ml @14:49:53.03 Intake.select :open @14:49:55.20 Intake @16.0psia, vacuum from 10.0 to 13.0psi @14:49:55.22 SS.reconfigure :inhaling 0.59ml/s @14:49:55.27 SS.seek 25ml @14:50:05.68 washCycleDA #1 of 3 @14:50:05.69 PSR.select :air @14:50:05.71 PSV.dial PSR @14:50:07.06 Pulling 0.5ml of PSR.wash @14:50:07.07 PSR.select :wash @14:50:07.19 PS.reconfigure :default @14:50:07.24 PS.seek 0.5ml @14:50:13.12 delay 5 seconds @14:50:18.14 PSR.select :air @14:50:18.26 PS.seek 2ml @14:50:34.40 PTV.dial :puck @14:50:35.15 PSV.dial PTV @14:50:35.92 PBV.dial :waste1 @14:50:36.07 Vacuum too high -- waiting up to 40 seconds @14:50:36.09 SS.reconfigure :exhale @14:50:36.15 SS.hold @14:50:36.36 PS.seek 1.5ml @14:50:42.30 delay 3 seconds @14:50:45.32 PBV.dial between PSV and waste1 @14:50:45.86 PS.seek 0ml @14:50:49.01 Intake @17.0psia, vacuum from 10.0 to 13.0psi @14:50:49.03 SS.reconfigure :inhaling 0.47ml/s @14:50:49.08 SS.seek 25ml @14:50:54.09 Stroke completed -- waiting for pressure to equalize ... @14:50:54.10 SS.reconfigure :exhale @14:50:54.15 SS.hold @14:51:02.21 delay 2:00 @14:51:10.56 Intake.select :close @14:51:11.13 SSV.dial :exhaust @14:51:11.90 Exhaust.select :open @14:51:12.02 SS.seek 0ml @14:51:21.06 Exhaust.select :close @14:51:21.18 SSV.dial CBV @14:51:21.20 CBV.dial SSV @14:51:22.00 Allowing 130s for 25.0ml of remaining 500.0ml @14:51:22.01 Intake.select :open @14:51:24.18 Intake @15.9psia, vacuum from 10.0 to 13.0psi @14:51:24.20 SS.reconfigure :inhaling 0.47ml/s @14:51:24.25 SS.seek 25ml @14:52:19.34 Stroke completed -- waiting for pressure to equalize ... @14:52:19.35 SS.reconfigure :exhale @14:52:19.40 SS.hold @14:52:43.93 Intake.select :close @14:52:44.09 SSV.dial :exhaust @14:52:44.87 Exhaust.select :open @14:52:44.99 SS.seek 0ml @14:52:53.85 Exhaust.select :close @14:52:53.96 SSV.dial CBV @14:52:54.01 CBV.dial SSV @14:52:54.75 Allowing 130s for 25.0ml of remaining 475.0ml @14:52:54.76 Intake.select :open @14:52:56.93 Intake @15.9psia, vacuum from 10.0 to 13.0psi @14:52:56.95 SS.reconfigure :inhaling 0.47ml/s @14:52:57.00 SS.seek 25ml @14:53:02.23 recoverPSRreagent08 @14:53:02.24 PTV.dial :puck @14:53:02.26 PBV.dial :waste1 @14:53:02.63 delay 30 seconds @14:53:32.66 PBV.dial between PSV and waste1 @14:53:33.04 PSR.select :air @14:53:33.06 PSV.dial PSR @14:53:33.82 PS.reconfigure :fast @14:53:33.87 PS.seek 2.5ml @14:53:52.65 Stroke completed -- waiting for pressure to equalize ... @14:53:52.66 SS.reconfigure :exhale @14:53:52.71 SS.hold @14:53:57.81 PSV.dial PTV @14:53:58.57 delay 5 seconds @14:54:03.59 PSV.dial PSR @14:54:04.35 delay 5 seconds @14:54:09.37 PSV.dial PTV @14:54:10.13 delay 5 seconds @14:54:15.15 PSV.dial :air @14:54:16.04 PS.seek 0ml @14:54:21.27 Intake.select :close @14:54:21.43 SSV.dial :exhaust @14:54:22.20 Exhaust.select :open @14:54:22.32 SS.seek 0ml @14:54:31.18 Exhaust.select :close @14:54:31.29 SSV.dial CBV @14:54:31.34 CBV.dial SSV @14:54:32.09 Allowing 130s for 25.0ml of remaining 450.0ml @14:54:32.10 Intake.select :open @14:54:34.27 Intake @16.0psia, vacuum from 10.0 to 13.0psi @14:54:34.29 SS.reconfigure :inhaling 0.47ml/s @14:54:34.34 SS.seek 25ml @14:54:39.55 washCycleDA #2 of 3 @14:54:39.56 PSR.select :air @14:54:39.58 PSV.dial PSR @14:54:40.93 Pulling 0.5ml of PSR.wash @14:54:40.94 PSR.select :wash @14:54:41.05 PS.reconfigure :default @14:54:41.10 PS.seek 0.5ml @14:54:46.99 delay 5 seconds @14:54:52.01 PSR.select :air @14:54:52.14 PS.seek 2ml @14:55:08.33 PTV.dial :puck @14:55:08.35 PSV.dial PTV @14:55:09.13 PBV.dial :waste1 @14:55:09.57 PS.seek 1.5ml @14:55:15.52 delay 3 seconds @14:55:18.54 PBV.dial between PSV and waste1 @14:55:18.92 PS.seek 0ml @14:55:30.44 Stroke completed -- waiting for pressure to equalize ... @14:55:30.46 SS.reconfigure :exhale @14:55:30.51 SS.hold @14:55:35.02 delay 2:00 @14:55:59.11 Intake.select :close @14:55:59.27 SSV.dial :exhaust @14:56:00.06 Exhaust.select :open @14:56:00.17 SS.seek 0ml @14:56:09.26 Exhaust.select :close @14:56:09.37 SSV.dial CBV @14:56:09.39 CBV.dial SSV @14:56:10.18 Allowing 130s for 25.0ml of remaining 425.0ml @14:56:10.19 Intake.select :open @14:56:12.36 Intake @16.1psia, vacuum from 10.0 to 13.0psi @14:56:12.38 SS.reconfigure :inhaling 0.47ml/s @14:56:12.43 SS.seek 25ml @14:57:04.38 Vacuum too high -- waiting up to 40 seconds @14:57:04.39 SS.reconfigure :exhale @14:57:04.45 SS.hold @14:57:23.46 Intake @18.1psia, vacuum from 10.0 to 13.0psi @14:57:23.48 SS.reconfigure :inhaling 0.38ml/s @14:57:23.53 SS.seek 25ml @14:57:27.79 Stroke completed -- waiting for pressure to equalize ... @14:57:27.80 SS.reconfigure :exhale @14:57:27.85 SS.hold @14:57:35.04 recoverPSRreagent08 @14:57:35.06 PTV.dial :puck @14:57:35.07 PBV.dial :waste1 @14:57:35.92 delay 30 seconds @14:57:48.67 Intake.select :close @14:57:48.83 SSV.dial :exhaust @14:57:49.61 Exhaust.select :open @14:57:49.73 SS.seek 0ml @14:57:58.52 Exhaust.select :close @14:57:58.64 SSV.dial CBV @14:57:58.66 CBV.dial SSV @14:57:59.46 Allowing 130s for 25.0ml of remaining 400.0ml @14:57:59.47 Intake.select :open @14:58:01.65 Intake @16.0psia, vacuum from 10.0 to 13.0psi @14:58:01.67 SS.reconfigure :inhaling 0.38ml/s @14:58:01.71 SS.seek 25ml @14:58:05.94 PBV.dial between PSV and waste1 @14:58:06.31 PSR.select :air @14:58:06.33 PSV.dial PSR @14:58:07.10 PS.reconfigure :fast @14:58:07.15 PS.seek 2.5ml @14:58:31.08 PSV.dial PTV @14:58:31.85 delay 5 seconds @14:58:36.87 PSV.dial PSR @14:58:37.64 delay 5 seconds @14:58:42.66 PSV.dial PTV @14:58:43.44 delay 5 seconds @14:58:48.46 PSV.dial :air @14:58:49.23 PS.seek 0ml @14:59:10.56 Stroke completed -- waiting for pressure to equalize ... @14:59:10.58 SS.reconfigure :exhale @14:59:10.63 SS.hold @14:59:12.61 washCycleDA #3 of 3 @14:59:12.62 PSR.select :air @14:59:12.64 PSV.dial PSR @14:59:13.98 Pulling 0.5ml of PSR.wash @14:59:13.99 PSR.select :wash @14:59:14.11 PS.reconfigure :default @14:59:14.16 PS.seek 0.5ml @14:59:20.08 delay 5 seconds @14:59:25.09 PSR.select :air @14:59:25.28 PS.seek 2ml @14:59:39.51 Intake.select :close @14:59:40.09 SSV.dial :exhaust @14:59:40.93 Exhaust.select :open @14:59:41.05 SS.seek 0ml @14:59:41.45 PTV.dial :puck @14:59:41.47 PSV.dial PTV @14:59:42.22 PBV.dial :waste1 @14:59:42.66 PS.seek 1.5ml @14:59:48.61 delay 3 seconds @14:59:49.94 Exhaust.select :close @14:59:50.06 SSV.dial CBV @14:59:50.08 CBV.dial SSV @14:59:50.88 Allowing 130s for 25.0ml of remaining 375.0ml @14:59:50.89 Intake.select :open @14:59:51.63 PBV.dial between PSV and waste1 @14:59:51.99 PS.seek 0ml @14:59:53.06 Intake @16.1psia, vacuum from 10.0 to 13.0psi @14:59:53.08 SS.reconfigure :inhaling 0.38ml/s @14:59:53.13 SS.seek 25ml @15:00:08.11 delay 2:00 @15:01:02.13 Stroke completed -- waiting for pressure to equalize ... @15:01:02.14 SS.reconfigure :exhale @15:01:02.19 SS.hold @15:01:34.97 Intake.select :close @15:01:35.12 SSV.dial :exhaust @15:01:35.91 Exhaust.select :open @15:01:36.03 SS.seek 0ml @15:01:45.14 Exhaust.select :close @15:01:45.26 SSV.dial CBV @15:01:45.28 CBV.dial SSV @15:01:46.08 Allowing 130s for 25.0ml of remaining 350.0ml @15:01:46.09 Intake.select :open @15:01:48.27 Intake @16.2psia, vacuum from 10.0 to 13.0psi @15:01:48.29 SS.reconfigure :inhaling 0.38ml/s @15:01:48.33 SS.seek 25ml @15:02:08.14 recoverPSRreagent08 @15:02:08.15 PTV.dial :puck @15:02:08.16 PBV.dial :waste1 @15:02:08.53 delay 30 seconds @15:02:38.56 PBV.dial between PSV and waste1 @15:02:38.93 PSR.select :air @15:02:38.95 PSV.dial PSR @15:02:39.72 PS.reconfigure :fast @15:02:39.77 PS.seek 2.5ml @15:02:56.50 Stroke completed -- waiting for pressure to equalize ... @15:02:56.51 SS.reconfigure :exhale @15:02:56.56 SS.hold @15:03:03.70 PSV.dial PTV @15:03:04.59 delay 5 seconds @15:03:09.61 PSV.dial PSR @15:03:10.36 delay 5 seconds @15:03:15.37 PSV.dial PTV @15:03:16.14 delay 5 seconds @15:03:21.15 PSV.dial :air @15:03:21.92 PS.seek 0ml @15:03:33.68 Intake.select :close @15:03:33.84 SSV.dial :exhaust @15:03:34.61 Exhaust.select :open @15:03:34.73 SS.seek 0ml @15:03:43.61 Exhaust.select :close @15:03:43.72 SSV.dial CBV @15:03:43.75 CBV.dial SSV @15:03:44.55 Allowing 130s for 25.0ml of remaining 325.0ml @15:03:44.56 Intake.select :open @15:03:45.45 Gate.power :camera,:ON @15:03:45.46 Mixing DA Substrates @15:03:45.47 PSR.select :air @15:03:45.49 PSV.dial PSR @15:03:46.82 Intake @16.1psia, vacuum from 10.0 to 13.0psi @15:03:46.88 SS.reconfigure :inhaling 0.38ml/s @15:03:47.06 SS.seek 25ml @15:03:47.16 Pulling 0.25ml of PSR.sub1 @15:03:47.22 PSR.select :sub1 @15:03:47.46 PS.reconfigure :default @15:03:47.58 PS.seek 0.25ml @15:03:51.04 delay 5 seconds @15:03:56.06 Pulling 0.25ml of PSR.sub2 @15:03:56.08 PSR.select :sub2 @15:03:56.20 PS.seek 0.5ml @15:03:59.60 delay 5 seconds @15:04:04.62 PSR.select :air @15:04:04.74 PS.seek 2ml @15:04:20.95 PTV.dial :puck @15:04:20.96 PSV.dial PTV @15:04:21.84 PBV.dial :waste1 @15:04:22.28 PS.seek 1.5ml @15:04:28.26 delay 3 seconds @15:04:31.28 PBV.dial between PSV and waste1 @15:04:31.66 PS.seek 0ml @15:04:43.90 Vacuum too high -- waiting up to 40 seconds @15:04:43.92 SS.reconfigure :exhale @15:04:43.97 SS.hold @15:04:47.78 delay 10 seconds @15:04:57.80 recoverPSRreagent08 @15:04:57.81 PTV.dial :puck @15:04:57.82 PBV.dial :waste1 @15:04:58.21 delay 30 seconds @15:05:00.76 Intake @17.0psia, vacuum from 10.0 to 13.0psi @15:05:00.78 SS.reconfigure :inhaling 0.31ml/s @15:05:00.83 SS.seek 25ml @15:05:16.85 Stroke completed -- waiting for pressure to equalize ... @15:05:16.87 SS.reconfigure :exhale @15:05:16.92 SS.hold @15:05:28.23 PBV.dial between PSV and waste1 @15:05:28.60 PSR.select :air @15:05:28.62 PSV.dial PSR @15:05:29.39 PS.reconfigure :fast @15:05:29.44 PS.seek 2.5ml @15:05:41.51 Intake.select :close @15:05:41.67 SSV.dial :exhaust @15:05:42.45 Exhaust.select :open @15:05:42.57 SS.seek 0ml @15:05:51.68 Exhaust.select :close @15:05:51.79 SSV.dial CBV @15:05:51.83 CBV.dial SSV @15:05:52.59 Allowing 130s for 25.0ml of remaining 300.0ml @15:05:52.60 Intake.select :open @15:05:53.63 PSV.dial PTV @15:05:54.42 delay 5 seconds @15:05:54.77 Intake @16.0psia, vacuum from 10.0 to 13.0psi @15:05:54.79 SS.reconfigure :inhaling 0.31ml/s @15:05:54.84 SS.seek 25ml @15:05:59.43 PSV.dial PSR @15:06:00.67 delay 5 seconds @15:06:05.70 PSV.dial PTV @15:06:06.48 delay 5 seconds @15:06:11.50 PSV.dial :air @15:06:12.26 PS.seek 0ml @15:06:36.16 Additional air clear @15:06:36.17 PSR.select :air @15:06:36.19 PSV.dial PSR @15:06:37.57 Pulling 2ml of PSR.air @15:06:37.58 PSR.select :air @15:06:37.60 PS.reconfigure :default @15:06:37.65 PS.seek 2ml @15:06:59.13 delay 5 seconds @15:07:04.15 PTV.dial :puck @15:07:04.17 PSV.dial PTV @15:07:04.93 PBV.dial :waste1 @15:07:05.37 PS.seek 1.5ml @15:07:11.32 delay 3 seconds @15:07:14.34 PBV.dial between PSV and waste1 @15:07:14.71 delay 1 second @15:07:15.73 PSV.dial :waste1 @15:07:16.83 PS.seek 0ml @15:07:22.45 Stroke completed -- waiting for pressure to equalize ... @15:07:22.46 SS.reconfigure :exhale @15:07:22.51 SS.hold @15:07:33.13 Processing Puck Heater at 28C before imaging @15:07:33.14 PH.coast @15:07:33.74 Imaging da puck in Processing Clamp while storing it to tube 4 @15:07:34.04 SC.reconfigure :tight @15:07:34.12 SC.seek 4 @15:07:37.68 SC.configure :limp @15:07:37.90 Elbow.reconfigure :tight @15:07:37.95 Elbow.seek 4 @15:07:39.69 Forearm.reconfigure :tight @15:07:39.74 Forearm.seek 4 + 450 counts @15:07:41.00 SE.reconfigure :pick @15:07:41.07 SE.seek :beamBreak @15:07:43.41 SE.configure :default @15:07:43.58 Elbow.reconfigure :limp @15:07:43.63 Forearm.reconfigure :loose @15:07:43.68 Forearm.seek 4 @15:07:43.93 Elbow.hold @15:07:44.00 Elbow.reconfigure :tight @15:07:44.10 SE.seek 14.13 Pucks below Top Plate @15:07:44.58 Forearm.force -20 @15:07:44.63 Forearm.seek 4 + 301 counts @15:07:44.91 Forearm.reconfigure :tight @15:07:44.96 Forearm.seek 4 + 450 counts @15:07:45.15 SE.seek 15.13 Pucks below Top Plate @15:07:45.72 Forearm.reconfigure :loose @15:07:46.27 Forearm.seek :retract @15:07:47.20 Elbow.seek PC @15:07:49.71 Elbow.reconfigure :loose @15:07:49.81 Forearm.seek PC @15:07:50.79 Hand.close @15:07:51.63 PC.seek :open @15:07:56.40 Forearm.seek :retract @15:07:57.36 Elbow.reconfigure :tight @15:07:57.41 Elbow.seek 4 @15:07:57.74 Intake.select :close @15:07:57.90 SSV.dial :exhaust @15:07:58.68 Exhaust.select :open @15:07:58.80 SS.seek 0ml @15:08:00.08 Forearm.reconfigure :tight @15:08:00.13 Forearm.seek 4 + 450 counts @15:08:01.15 Hand.open @15:08:01.87 SE.seek 14.78 Pucks below Top Plate @15:08:02.13 SE.reconfigure :seal @15:08:02.19 SE.seek 13.81 Pucks below Top Plate @15:08:02.70 Lights, camera, ACTION! @15:08:02.73 PuckCamera: snap -bin=2,2 -auto /var/log/esp/da23dec0515h1000ml.tif @15:08:07.90 Exhaust.select :close @15:08:08.02 SSV.dial CBV @15:08:08.07 CBV.dial SSV @15:08:08.82 Allowing 130s for 25.0ml of remaining 275.0ml @15:08:08.84 Intake.select :open @15:08:11.01 Intake @16.1psia, vacuum from 10.0 to 13.0psi @15:08:11.03 SS.reconfigure :inhaling 0.31ml/s @15:08:11.09 SS.seek 25ml @15:08:22.24 Waiting >20 seconds for da to relinquish FlushPuck @15:08:23.03 SX HX9/16 (USB:16): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 17.006 seconds /var/log/esp/da23dec0515h1000ml.tif: TIFF Upload Complete @15:08:23.62 PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da23dec0515h1000ml15s.tif @15:08:39.27 SX HX9/16 (USB:16): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15 seconds /var/log/esp/midres/da23dec0515h1000ml15s.tif: TIFF Upload Complete @15:08:39.30 PuckCamera: snap -bin=2,2 20.0 /var/log/esp/midres/da23dec0515h1000ml20s.tif @15:08:59.91 SX HX9/16 (USB:16): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 20 seconds /var/log/esp/midres/da23dec0515h1000ml20s.tif: TIFF Upload Complete @15:08:59.93 Gate.power :camera,:OFF @15:08:59.95 DAfans.email "17.006 sec auto-exposure",Subject: "da23dec0515h1000ml images!" @15:09:00.44 Elbow.seek 4 @15:09:00.66 Forearm.seek 4 + 450 counts @15:09:00.91 SE.reconfigure :gently @15:09:00.96 SE.seek 14.53 Pucks below Top Plate @15:09:01.61 Elbow.reconfigure :limp @15:09:01.66 Forearm.reconfigure :loose @15:09:01.71 Forearm.seek 4 @15:09:01.96 Elbow.hold @15:09:02.04 Elbow.reconfigure :tight @15:09:02.10 SE.reconfigure :default @15:09:02.16 SE.seek 14.72 Pucks below Top Plate @15:09:02.57 Forearm.force -20 @15:09:02.61 Forearm.seek 4 + 312 counts @15:09:02.92 Forearm.reconfigure :tight @15:09:02.97 Forearm.seek 4 + 450 counts @15:09:03.25 SE.seek :down @15:09:04.52 Forearm.reconfigure :loose @15:09:04.57 Forearm.seek :retract @15:09:05.02 SE.stop @15:09:05.74 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @15:09:06.17 Elbow.seek FlushPuck::Garage @15:09:08.20 Elbow.reconfigure :loose @15:09:08.41 Forearm.seek :garage @15:09:09.42 Hand.close @15:09:11.46 Forearm.seek :retract @15:09:12.49 Elbow.reconfigure :tight @15:09:12.59 Elbow.seek PC @15:09:13.95 Elbow.reconfigure :loose @15:09:14.17 Forearm.reconfigure :tight @15:09:14.26 Forearm.seek PC @15:09:15.87 PC.seek :closed @15:09:20.68 Processing Clamp onSpring/offSpring for 16/26 samples, on/off torque 1.63 @15:09:20.83 Hand.open @15:09:21.67 Forearm.reconfigure :loose @15:09:21.77 Forearm.seek :retract @15:09:22.77 PSR.select :air @15:09:22.81 PSV.dial PSR @15:09:23.37 Pulling 0.5ml of PSR.flush @15:09:23.40 PSR.select :flush @15:09:23.61 PS.seek 0.5ml @15:09:29.62 delay 5 seconds @15:09:34.10 upsync @15:09:34.64 PSR.select :air @15:09:34.76 PS.seek 2.5ml @15:09:39.86 Stroke completed -- waiting for pressure to equalize ... @15:09:39.87 SS.reconfigure :exhale @15:09:39.92 SS.hold @15:09:56.03 delay 1 second @15:09:57.06 PRV.dial :waste2,avoiding: :ab2 @15:09:58.94 PTV.dial :PRVmixing @15:10:00.28 PSV.dial :mixloop @15:10:01.33 PS.seek :empty @15:10:18.89 SS.reconfigure :default @15:10:18.95 SS.hold @15:10:19.01 Intake.select :close @15:10:19.64 ShallowSampler::Clogged in WCR -- Filtering too Slowly after 750.0ml @15:10:19.65 SSV.dial :exhaust @15:10:20.41 Exhaust.select :open @15:10:20.53 SS.reconfigure :exhale @15:10:20.59 SS.seek 0ml @15:10:27.69 PSR.select :air @15:10:27.71 PSV.dial PSR @15:10:28.77 Pulling 0.5ml of PSR.flush @15:10:28.78 PSR.select :flush @15:10:28.90 PS.seek 0.5ml @15:10:29.49 Exhaust.select :close @15:10:29.60 Sampled 750.0ml @15:10:29.62 Drying puck before fixing for 5:00 @15:10:29.63 CBV.dial :waste2b @15:10:30.39 delay 1:00 @15:10:34.82 delay 5 seconds @15:10:39.84 PSR.select :air @15:10:39.96 PS.seek 2.5ml @15:11:01.25 delay 1 second @15:11:02.27 PBV.dial :waste1 @15:11:02.64 PTV.dial :mixing @15:11:03.41 PSV.dial :mixloop @15:11:04.48 PS.seek :empty @15:11:30.41 CSV.dial :air @15:11:30.84 PSV.dial PSR @15:11:30.92 CS.reconfigure :fast @15:11:30.98 CS.seek 10ml @15:11:31.91 PSR.select :flush @15:11:32.03 PS.seek 1ml @15:11:43.09 PSR.select :air @15:11:43.21 PS.seek 2.5ml @15:11:55.51 Pumping wcr puck from tube 5 up to 35psi... @15:11:55.53 CBV.dial SSV @15:11:56.80 CBV.dial between CSV and waste2b @15:11:57.77 CSV.dial between air and CTV @15:11:58.10 CS.seek 7.123ml @15:11:59.44 PSV.dial PTV @15:12:00.20 PTV.dial :puck @15:12:00.95 PBV.dial :waste1 @15:12:01.04 PS.seek 0ml @15:12:05.49 CTV.dial CSV,avoiding: :air @15:12:05.51 CSV.dial CTV @15:12:06.33 CS.reconfigure :pressurize @15:12:06.38 CS.seek 0ml @15:12:24.85 CBV.dial :waste2b @15:12:25.19 CS.reconfigure :default @15:12:25.24 CS.seek 0ml @15:12:26.64 delay 1:00 @15:12:27.41 PSV.dial PSR @15:12:28.16 PSR.select :flush @15:12:28.28 PS.seek 0.5ml @15:12:34.19 PSR.select :air @15:12:34.30 PS.seek 2.5ml @15:12:55.56 PSV.dial PTV @15:12:56.33 PBV.dial PSV @15:12:56.90 PS.seek 0ml @15:13:23.22 PSV.dial PBV @15:13:23.69 PS.seek 2.5ml @15:13:26.66 CTV.dial :air,avoiding: :intake @15:13:27.98 Fix #1 of 2 (with RNAlater) for 2:30 @15:13:28.00 CSV.dial CSR @15:13:28.43 Pulling 2ml of CSR.RNAlater @15:13:28.44 CSR.select :RNAlater @15:13:28.56 CS.seek 2ml @15:13:34.57 delay 5 seconds @15:13:39.59 CSR.select :air @15:13:39.72 CS.reconfigure :fast @15:13:39.77 CS.seek 10ml @15:13:50.09 PSV.dial :waste1 @15:13:50.83 PS.seek 0ml @15:13:59.39 Pumping wcr puck from tube 5 up to 35psi... @15:13:59.40 CBV.dial SSV @15:14:00.67 CBV.dial between CSV and waste2b @15:14:01.65 CSV.dial between air and CTV @15:14:02.25 CS.seek 7.028ml @15:14:09.89 CTV.dial CSV,avoiding: :air @15:14:09.91 CSV.dial CTV @15:14:11.30 CS.reconfigure :pressurize @15:14:11.35 CS.seek 0ml @15:14:17.18 PSV.dial PSR @15:14:17.65 PSR.select :air @15:14:17.68 PS.seek 2.5ml @15:14:25.38 delay 2:30 @15:14:44.03 PSV.dial PTV @15:14:44.76 PBV.dial PSV @15:14:45.29 PS.seek 0ml @15:15:11.62 PSV.dial PBV @15:15:12.10 PS.seek 2.5ml @15:15:38.62 PSV.dial :waste1 @15:15:39.36 PS.seek 0ml @15:16:05.71 PSV.dial PSR @15:16:06.18 PSR.select :flush @15:16:06.31 PS.seek 1ml @15:16:17.31 PSR.select :air @15:16:17.43 PS.seek 2.5ml @15:16:33.56 PSV.dial PTV @15:16:34.29 PTV.dial :puck @15:16:34.37 PBV.dial :waste1 @15:16:34.94 PS.seek 0ml @15:16:55.40 CBV.dial :waste2b @15:16:55.75 CS.reconfigure :default @15:16:55.80 CS.seek 0ml @15:17:00.98 delay 1:00 @15:17:01.43 PSV.dial PSR @15:17:02.18 PSR.select :flush @15:17:02.31 PS.seek 0.5ml @15:17:08.27 PSR.select :air @15:17:08.40 PS.seek 2.5ml @15:17:29.59 PSV.dial PTV @15:17:30.35 PBV.dial PSV @15:17:30.92 PS.seek 0ml @15:17:57.31 PSV.dial PBV @15:17:57.78 PS.seek 2.5ml @15:18:01.00 CTV.dial :air,avoiding: :intake @15:18:02.35 Fix #2 of 2 (with RNAlater) for 2:30 @15:18:02.36 CSV.dial CSR @15:18:02.80 Pulling 2ml of CSR.RNAlater @15:18:02.81 CSR.select :RNAlater @15:18:02.93 CS.seek 2ml @15:18:08.94 delay 5 seconds @15:18:13.96 CSR.select :air @15:18:14.08 CS.reconfigure :fast @15:18:14.14 CS.seek 10ml @15:18:24.12 PSV.dial :waste1 @15:18:24.86 PS.seek 0ml @15:18:33.85 Pumping wcr puck from tube 5 up to 35psi... @15:18:33.87 CBV.dial SSV @15:18:35.12 CBV.dial between CSV and waste2b @15:18:36.55 CSV.dial between air and CTV @15:18:37.15 CS.seek 6.982ml @15:18:44.89 CTV.dial CSV,avoiding: :air @15:18:44.91 CSV.dial CTV @15:18:46.31 CS.reconfigure :pressurize @15:18:46.36 CS.seek 0ml @15:18:51.24 PSV.dial PSR @15:18:51.71 PSR.select :air @15:18:51.74 PS.seek 2.5ml @15:19:03.87 delay 2:30 @15:19:18.27 PSV.dial PTV @15:19:19.13 PBV.dial PSV @15:19:19.22 PS.seek 0ml @15:19:45.55 PSV.dial PBV @15:19:46.01 PS.seek 2.5ml @15:20:12.30 PSV.dial :waste1 @15:20:13.06 PS.seek 0ml @15:20:39.56 Garaging FlushPuck in Processing Clamp @15:20:39.63 Elbow.reconfigure :tight @15:20:39.68 Elbow.seek PC @15:20:39.81 Elbow.reconfigure :loose @15:20:39.91 Forearm.seek PC @15:20:40.90 Hand.close @15:20:41.55 PC.seek :open @15:20:46.27 Forearm.seek :retract @15:20:47.22 Elbow.reconfigure :tight @15:20:47.27 Elbow.seek :garage @15:20:48.62 Elbow.reconfigure :loose @15:20:48.72 Forearm.reconfigure :tight @15:20:48.77 Forearm.seek :garage @15:20:49.73 Hand.open @15:20:50.36 Forearm.reconfigure :loose @15:20:50.41 Forearm.seek :retract @15:21:33.89 CBV.dial :waste2b @15:21:34.23 CS.reconfigure :default @15:21:34.28 CS.seek 0ml @15:21:35.88 delay 1:00 @15:22:35.90 CTV.dial :air,avoiding: :intake @15:22:37.70 Storing wcr puck in Collection Clamp to tube 4 @15:22:38.13 Elbow.reconfigure :tight @15:22:38.18 Elbow.seek 4 @15:22:40.06 Forearm.reconfigure :tight @15:22:40.11 Forearm.seek 4 + 450 counts @15:22:41.15 SE.reconfigure :pick @15:22:41.21 SE.seek :beamBreak @15:22:43.34 SE.configure :default @15:22:43.51 Elbow.reconfigure :limp @15:22:43.56 Forearm.reconfigure :loose @15:22:43.61 Forearm.seek 4 @15:22:43.82 Elbow.hold @15:22:43.89 Elbow.reconfigure :tight @15:22:44.00 SE.seek 15.12 Pucks below Top Plate @15:22:44.47 Forearm.force -20 @15:22:44.52 Forearm.seek 4 + 317 counts @15:22:44.81 Forearm.reconfigure :tight @15:22:44.87 Forearm.seek 4 + 450 counts @15:22:45.07 SE.seek 16.12 Pucks below Top Plate @15:22:45.74 Forearm.reconfigure :loose @15:22:45.79 Forearm.seek :retract @15:22:46.73 Elbow.seek CC @15:22:48.38 Elbow.reconfigure :loose @15:22:49.03 Forearm.seek CC @15:22:49.98 Hand.close @15:22:50.55 CC.seek :open @15:22:55.31 Forearm.seek :retract @15:22:56.33 Elbow.reconfigure :tight @15:22:56.38 Elbow.seek 4 @15:22:58.15 Elbow.reconfigure :limp @15:22:58.20 Forearm.seek 4 @15:22:59.17 Elbow.hold @15:22:59.24 Elbow.reconfigure :tight @15:22:59.29 Hand.open @15:23:00.04 Forearm.force -20 @15:23:00.09 Forearm.seek 4 + 316 counts @15:23:00.59 Forearm.reconfigure :tight @15:23:00.64 Forearm.seek 4 + 450 counts @15:23:01.02 SE.seek :down @15:23:02.15 Forearm.reconfigure :loose @15:23:02.21 Forearm.seek :retract @15:23:02.63 SE.stop @15:23:03.14
Starting finalPurge @15:23:03.15 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @15:23:03.34 Elbow.seek FlushPuck::Garage @15:23:05.19 Elbow.reconfigure :loose @15:23:05.29 Forearm.seek :garage @15:23:06.28 Hand.close @15:23:07.48 Forearm.seek :retract @15:23:08.48 Elbow.reconfigure :tight @15:23:08.53 Elbow.seek CC @15:23:11.48 Elbow.reconfigure :loose @15:23:11.58 Forearm.reconfigure :tight @15:23:11.63 Forearm.seek CC @15:23:12.93 CC.seek :closed @15:23:17.45 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.34 @15:23:17.51 Hand.open @15:23:18.23 Forearm.reconfigure :loose @15:23:18.28 Forearm.seek :retract @15:23:19.22 Flushing the Collection Valve::Manifold @15:23:19.34 CSV.dial CSR @15:23:19.78 Pulling 5ml of CSR.flush @15:23:19.79 CSR.select :flush @15:23:19.91 CS.seek 5ml @15:23:33.69 delay 5 seconds @15:23:38.71 CSR.select :air @15:23:38.87 CS.reconfigure :fast @15:23:38.92 CS.seek 7.5ml @15:23:45.69 CSV.dial :waste @15:23:46.99 CS.reconfigure :default @15:23:47.04 CS.seek 0ml @15:24:07.06 Flushing lower manifold's waste1 port @15:24:07.19 CSV.dial :flush @15:24:07.63 CS.reconfigure :fast @15:24:07.68 CS.seek 5ml @15:24:20.56 delay 5 seconds @15:24:25.58 CSV.dial :air @15:24:26.11 CS.seek 7ml @15:24:31.75 CSV.dial CTV @15:24:31.78
CTV.dial CSV @15:24:33.18 CBV.dial :waste1 @15:24:33.94 CS.seek 0ml @15:24:50.53 Flushing lower manifold's waste2 port @15:24:50.66 CSV.dial :flush @15:24:51.51 CS.seek 5ml @15:25:04.40 delay 5 seconds @15:25:09.42 CSV.dial :air @15:25:09.94 CS.seek 6.999ml @15:25:15.41 CSV.dial CTV @15:25:15.43
CTV.dial CSV @15:25:15.89 CBV.dial :waste2 @15:25:16.94 CS.seek 0ml @15:25:33.50 Flushing lower manifold's waste2b port @15:25:33.62 CSV.dial :flush @15:25:34.36 CS.seek 5ml @15:25:47.22 delay 5 seconds @15:25:52.24 CSV.dial :air @15:25:52.76 CS.seek 7ml @15:25:58.25 CSV.dial CTV @15:25:58.27
CTV.dial CSV @15:25:58.73 CBV.dial :waste2b @15:25:59.79 CS.seek 0ml @15:26:16.34 Disinfecting external Intake with kill @15:26:16.48 CSV.dial CSR @15:26:17.04 Pulling 8ml of CSR.kill @15:26:17.05 CSR.select :kill @15:26:17.18 CS.seek 8ml @15:26:37.09 delay 5 seconds @15:26:42.11 CSR.select :air @15:26:42.23 CS.seek 10ml @15:26:47.75 CSV.dial CBV @15:26:47.80
CBV.dial CSV @15:26:48.95 CTV.dial :intake @15:26:49.73 Intake.select :open @15:26:49.84 CS.seek 2ml @15:27:08.87 Intake.select :close @15:27:08.98 CTV.dial :air @15:27:09.73 CS.reconfigure :default @15:27:09.78 CS.seek 0ml @15:27:15.65 CSV.dial CSR @15:27:16.67 Pulling 5ml of CSR.flush @15:27:16.68 CSR.select :flush @15:27:16.85 CS.seek 5ml @15:27:30.78 delay 5 seconds @15:27:35.84 CSR.select :air @15:27:35.96 CS.seek 10ml @15:27:49.53 CSV.dial CBV @15:27:50.55 CS.seek 0ml @15:28:17.34 Garaging FlushPuck in Collection Clamp @15:28:17.41 Elbow.reconfigure :tight @15:28:17.46 Elbow.seek CC @15:28:17.79 Elbow.reconfigure :loose @15:28:17.89 Forearm.seek CC @15:28:18.91 Hand.close @15:28:19.54 CC.seek :open @15:28:24.18 Forearm.seek :retract @15:28:25.18 Elbow.reconfigure :tight @15:28:25.23 Elbow.seek :garage @15:28:28.24 Elbow.reconfigure :loose @15:28:28.34 Forearm.reconfigure :tight @15:28:28.39 Forearm.seek :garage @15:28:30.08 Hand.open @15:28:30.72 Forearm.reconfigure :loose @15:28:30.77 Forearm.seek :retract @15:28:31.74 Completed [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] begun 12:43:35 @15:28:31.80 Asleep after completing DA_WCR_120523 assays @15:28:31.82 GoodNews.email "{1 => 33,\n 2 => 33,\n 3 => 31,\n 4 => 17,\n 5 => 16,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@15:22:55, 30% flow, 17.1C, 31% humidity, 15.1psia, 13.715V, 0.411A, 154.680Ah, 5.64W\nSampled 750.0ml",Subject: "Asleep after completing DA_WCR_120523 assays" @15:28:36.31 upsync + ESPwhich DA_WCR_120523b.rb + fn=DA_WCR_120523b.rb + IFS=: + set /home/esp/esp2/mission . + fullPath=/home/esp/esp2/mission/DA_WCR_120523b.rb + [ -f /home/esp/esp2/mission/DA_WCR_120523b.rb ] + realpath /home/esp/esp2/mission/DA_WCR_120523b.rb + return 0 + scriptPath=/home/esp/esp2local/mission/DA_WCR_120523b.rb + exec ruby /home/esp/esp2/lib/esp.rb /home/esp/esp2local/mission/DA_WCR_120523b.rb @21:15:55.18UTC05-Dec-23
ESP2 v3.42 I2C Gateway v3.20 for core bus @21:15:57.91 Configuration for short puck jake 1/11/21 by brent@mbari.org /home/esp/esp2/type/shallow/jake/configure.rb @21:16:01.55 Polling Can every 10:00 @21:16:01.60 Cleanly exited at 18:51:46UTC05-Dec-23 @21:16:01.64 Loading /home/esp/esp2local/mission/DA_WCR_120523b.rb mission @21:16:01.70 GoodNews.email "Starting DA_WCR_120523b w/pucks in tube 5\n{1 => 33,\n 2 => 33,\n 3 => 31,\n 4 => 17,\n 5 => 16,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@21:16:01, 31% flow, 16.5C, 32% humidity, 15.1psia, 13.749V, 0.278A, 156.299Ah, 3.83W",Subject: "DA_WCR_120523b begun" @21:16:01.73
Preparing to sleep until + 00:00:05 @21:16:01.78 Asleep til + 00:00:05 @21:16:01.79 Continuing at 21:16 @21:16:02.08 Gate.power :core,:OFF @21:16:02.09 Gate.power :raw,:OFF @21:16:02.12 Shutdown @21:16:05.11 GoodNews.email "Then, in tube 5: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_120523b.rb:14\n{1 => 33,\n 2 => 33,\n 3 => 31,\n 4 => 17,\n 5 => 16,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@21:16:01, 31% flow, 16.5C, 32% humidity, 15.1psia, 13.749V, 0.278A, 156.299Ah, 3.83W",Subject: "Asleep until 21:16" @21:16:06.50 upsync @21:16:06.69
Powering core bus @21:16:09.92 upsync @21:16:12.45
Gate.power :raw,:ON @21:16:13.30 CS.configure :default @21:16:13.35 PS.configure :default @21:16:13.40 SS.configure :default @21:16:13.44 CC.configure :default @21:16:13.49 PC.configure :default @21:16:13.56 Forearm.configure :loose @21:16:13.61 Elbow.configure :loose @21:16:13.67 SC.configure :loose @21:16:13.73 SE.configure :default @21:16:13.77 CH.configure :default @21:16:13.81 PH.configure :default @21:16:13.85 All dwarf controllers running firmware version 3.85 @21:16:13.95 Valve.reset @21:16:14.30 PSV.dial :air @21:16:14.34 Intake.select :close @21:16:14.38 Exhaust.select :close @21:16:14.41 CTV.dial :air @21:16:14.48
Forearm.home.seek :retract @21:16:14.54 CSV.dial :waste1 @21:16:14.98 SSV.dial :spit,avoiding: :exhaust @21:16:15.26 Hand.close @21:16:15.31
Elbow.reconfigure :loose @21:16:15.39 Elbow.jog 1500 @21:16:15.46 PS.home.seek 0ml @21:16:15.85 CS.reconfigure :fast @21:16:15.89 SS.home.seek 0ml @21:16:15.98 CS.home.seek 0ml @21:16:16.05
Elbow.reconfigure :loose @21:16:16.12 Elbow.home.seek :clear @21:16:19.40 SE.home.seek :down @21:16:20.01 SC.reconfigure :tight @21:16:20.06 SC.home.seek :initial @21:16:23.93 PS.stop @21:16:38.97
SC.configure :limp @21:16:39.03 Forearm.seek :retract @21:16:39.10 Elbow.seek :hammer @21:16:40.13 Forearm.reconfigure :tight @21:16:40.18 Forearm.seek retracted @21:16:40.45 PC.home.seek :open @21:16:40.47
CC.home.seek :open @21:16:44.22 Phase [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] @21:16:44.23 Notice.email "At 21:16:44 began in tube 5: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_120523b.rb:14\n{1 => 33,\n 2 => 33,\n 3 => 31,\n 4 => 17,\n 5 => 16,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@21:16:44, 31% flow, 16.6C, 32% humidity, 15.1psia, 13.676V, 0.315A, 156.304Ah, 4.30W",Subject: "Started DA_WCR_120523b phase initialPurge; da [1000, 1000] #da and wsr are run simultanelously" @21:16:44.26
Starting initialPurge @21:16:44.27 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @21:16:44.33 Hand.open @21:16:45.13 Elbow.reconfigure :tight @21:16:45.17 Elbow.seek FlushPuck::Garage @21:16:47.05 Elbow.reconfigure :loose @21:16:47.16 Forearm.reconfigure :loose @21:16:47.32 Forearm.seek :garage @21:16:48.33 Hand.close @21:16:48.67 upsync @21:16:49.13
Forearm.seek :retract @21:16:50.11 Elbow.reconfigure :tight @21:16:50.16 Elbow.seek CC @21:16:53.18 Elbow.reconfigure :loose @21:16:53.29 Forearm.reconfigure :tight @21:16:53.34 Forearm.seek CC @21:16:54.57 CC.seek :closed @21:16:59.20 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.48 @21:16:59.26 Hand.open @21:16:59.93 Forearm.reconfigure :loose @21:16:59.98 Forearm.seek :retract @21:17:00.98 Flushing the Collection Valve::Manifold @21:17:01.11 CSV.dial CSR @21:17:02.40 Pulling 5ml of CSR.flush @21:17:02.41 CSR.select :flush @21:17:02.53 CS.reconfigure :default @21:17:02.98 CS.seek 5ml @21:17:16.57 delay 5 seconds @21:17:21.59 CSR.select :air @21:17:21.76 CS.reconfigure :fast @21:17:21.81 CS.seek 7.499ml @21:17:28.57 CSV.dial :waste @21:17:29.86 CS.reconfigure :default @21:17:29.91 CS.seek 0ml @21:17:49.92 Purging external Intake @21:17:50.04 CSV.dial :flush @21:17:50.61 CS.reconfigure :fast @21:17:50.66 CS.seek 10ml @21:18:15.13 delay 5 seconds @21:18:20.16 CSV.dial CBV @21:18:20.20
CBV.dial CSV @21:18:20.90 CTV.dial :intake @21:18:21.68 Intake.select :open @21:18:21.79 CS.seek 0ml @21:18:45.26 Intake.select :close @21:18:45.36 CTV.dial :air @21:18:46.14 Flushing upper manifold @21:18:46.26 CSV.dial :flush @21:18:47.00 CS.seek 5ml @21:19:00.01 delay 5 seconds @21:19:05.03 CSV.dial :air @21:19:05.54 CS.seek 7ml @21:19:11.13 CSV.dial CBV @21:19:11.16
CBV.dial CSV @21:19:12.20 CTV.dial :air @21:19:12.22 CS.seek 0ml @21:19:28.79 delay 7 seconds @21:19:35.81 CS.seek 3ml @21:19:44.04 CSV.dial :waste @21:19:44.50 CS.seek 0ml @21:19:52.30 CSV.dial :flush @21:19:52.76 CS.seek 3ml @21:20:00.69 delay 5 seconds @21:20:05.71 CSV.dial :air @21:20:06.21 CS.seek 5ml @21:20:11.94 CSV.dial :air @21:20:12.12 CS.seek 0ml @21:20:24.32 delay 7 seconds @21:20:31.35 CS.seek 3ml @21:20:39.57 CSV.dial :waste @21:20:40.29 CS.seek 0ml @21:20:47.98 Garaging FlushPuck in Collection Clamp @21:20:48.05 Elbow.reconfigure :tight @21:20:48.09 Elbow.seek CC @21:20:48.39 Elbow.reconfigure :loose @21:20:48.49 Forearm.seek CC @21:20:49.51 Hand.close @21:20:50.13 CC.seek :open @21:20:55.18 Forearm.seek :retract @21:20:56.15 Elbow.reconfigure :tight @21:20:56.20 Elbow.seek :garage @21:20:59.18 Elbow.reconfigure :loose @21:20:59.28 Forearm.reconfigure :tight @21:20:59.33 Forearm.seek :garage @21:21:00.34 Hand.open @21:21:01.06 Forearm.reconfigure :loose @21:21:01.10 Forearm.seek :retract @21:21:02.14 da sampling at most 1000ml, wcr at most 1000ml @21:21:02.18 Loading sh1 puck from tube 5 into Collection Clamp @21:21:02.19 DAfans.email "Started da in tube 5 at 21:21:02UTC05-Dec-23\n{1 => 33,\n 2 => 33,\n 3 => 31,\n 4 => 17,\n 5 => 16,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@21:16:44, 31% flow, 16.6C, 32% humidity, 15.1psia, 13.676V, 0.315A, 156.304Ah, 4.30W",Subject: "da sampling at most 1000ml, wcr at most 1000ml" @21:21:02.46
SC.reconfigure :tight @21:21:02.53 SC.seek 5 @21:21:06.09 SC.configure :limp @21:21:06.35 Elbow.reconfigure :tight @21:21:06.39 Elbow.seek 5 @21:21:06.56 upsync @21:21:08.38
Forearm.reconfigure :tight @21:21:08.44 Forearm.seek 5 + 450 counts @21:21:09.48 SE.reconfigure :pick @21:21:09.54 SE.seek :beamBreak @21:21:11.78 SE.configure :default @21:21:11.94 Elbow.seek 5 @21:21:12.13 Forearm.seek 5 + 300 counts @21:21:12.34 Forearm.reconfigure :loose @21:21:12.38 Hand.close @21:21:13.01 SE.seek :down @21:21:13.22
Forearm.seek :retract @21:21:14.21 Elbow.seek CC @21:21:14.75 SE.stop @21:21:15.79
Elbow.reconfigure :loose @21:21:15.89 Forearm.reconfigure :tight @21:21:15.94 Forearm.seek CC @21:21:17.32 CC.seek :closed @21:21:21.86 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.41 @21:21:21.87 Hand.open @21:21:22.55 Forearm.reconfigure :loose @21:21:22.60 Forearm.seek :retract @21:21:23.54 collectSample 1000ml w/bubble point of 24psi @21:21:23.56 Priming 7ml @21:21:23.57 Intake.select :close @21:21:23.58 Exhaust.select :close @21:21:23.59 SS.hold @21:21:23.79 Calibrating pressure and temperature sensors @21:21:23.82 CTV.dial :air @21:21:23.83 SSV.dial :spit,avoiding: :exhaust @21:21:23.91 SS.reconfigure :fast @21:21:23.96 SS.seek 5ml @21:21:26.77 SSV.dial CBV @21:21:26.79
CBV.dial SSV @21:21:28.13 delay 3.8 seconds @21:21:32.05 Sensor limits: Intake max 102.0psia; Outlet max 94.9psia @21:21:32.06 SSV.dial :spit @21:21:33.39 SS.seek 0ml @21:21:36.14 SSV.dial CBV @21:21:37.45 CTV.dial :intake @21:21:38.23 SSV.dial CBV @21:21:38.25
CBV.dial SSV @21:21:38.40 Allowing 40s for 7.0ml of remaining 7.0ml @21:21:38.41 Intake.select :open @21:21:40.57 Intake @14.6psia, vacuum from 9.1 to 12.1psi @21:21:40.58 SS.reconfigure :inhaling 2.20ml/s @21:21:40.63 SS.seek 7ml @21:21:47.53 Stroke completed -- waiting for pressure to equalize ... @21:21:47.54 SS.reconfigure :exhale @21:21:47.59 SS.hold @21:21:56.46 Intake.select :close @21:21:56.62 SSV.dial :exhaust @21:21:57.36 Exhaust.select :open @21:21:57.47 SS.seek 0ml @21:22:01.51 Exhaust.select :close @21:22:01.62 Sampling 1000.0ml @21:22:01.64 Intake.select :close @21:22:01.65 Exhaust.select :close @21:22:01.66 SS.reconfigure :default @21:22:01.70 SS.hold @21:22:01.91 CTV.dial :intake @21:22:02.15 SSV.dial CBV @21:22:02.17
CBV.dial SSV @21:22:02.94 Allowing 130s for 25.0ml of remaining 1000.0ml @21:22:02.95 Intake.select :open @21:22:05.11 Intake @15.4psia, vacuum from 9.9 to 12.9psi @21:22:05.13 SS.reconfigure :inhaling 2.20ml/s @21:22:05.18 SS.seek 25ml @21:22:13.82 Vacuum too high -- waiting up to 40 seconds @21:22:13.83 SS.reconfigure :exhale @21:22:13.89 SS.hold @21:22:20.20 Intake @18.5psia, vacuum from 10.0 to 13.0psi @21:22:20.29 SS.reconfigure :inhaling 1.76ml/s @21:22:20.34 SS.seek 25ml @21:22:22.98 Vacuum too high -- waiting up to 40 seconds @21:22:22.99 SS.reconfigure :exhale @21:22:23.04 SS.hold @21:22:24.68 Intake @17.2psia, vacuum from 10.0 to 13.0psi @21:22:24.70 SS.reconfigure :inhaling 1.41ml/s @21:22:24.74 SS.seek 25ml @21:22:29.23 Vacuum too high -- waiting up to 40 seconds @21:22:29.25 SS.reconfigure :exhale @21:22:29.30 SS.hold @21:22:31.90 Intake @18.1psia, vacuum from 10.0 to 13.0psi @21:22:31.91 SS.reconfigure :inhaling 1.13ml/s @21:22:31.96 SS.seek 25ml @21:22:36.48 Stroke completed -- waiting for pressure to equalize ... @21:22:36.49 SS.reconfigure :exhale @21:22:36.54 SS.hold @21:23:03.39 Intake.select :close @21:23:03.54 SSV.dial :exhaust @21:23:04.30 Exhaust.select :open @21:23:04.42 SS.seek 0ml @21:23:13.28 Exhaust.select :close @21:23:13.39 SSV.dial CBV @21:23:13.41
CBV.dial SSV @21:23:14.19 Allowing 130s for 25.0ml of remaining 975.0ml @21:23:14.20 Intake.select :open @21:23:16.36 Intake @16.0psia, vacuum from 10.0 to 13.0psi @21:23:16.37 SS.reconfigure :inhaling 1.13ml/s @21:23:16.42 SS.seek 25ml @21:23:41.25 Stroke completed -- waiting for pressure to equalize ... @21:23:41.26 SS.reconfigure :exhale @21:23:41.30 SS.hold @21:24:14.01 Intake.select :close @21:24:14.16 SSV.dial :exhaust @21:24:14.92 Exhaust.select :open @21:24:15.03 SS.seek 0ml @21:24:24.04 Exhaust.select :close @21:24:24.16 SSV.dial CBV @21:24:24.20
CBV.dial SSV @21:24:24.94 Allowing 130s for 25.0ml of remaining 950.0ml @21:24:24.95 Intake.select :open @21:24:27.11 Intake @16.0psia, vacuum from 10.0 to 13.0psi @21:24:27.12 SS.reconfigure :inhaling 1.13ml/s @21:24:27.17 SS.seek 25ml @21:24:51.89 Stroke completed -- waiting for pressure to equalize ... @21:24:51.90 SS.reconfigure :exhale @21:24:51.94 SS.hold @21:25:22.55 Intake.select :close @21:25:22.70 SSV.dial :exhaust @21:25:23.47 Exhaust.select :open @21:25:23.58 SS.seek 0ml @21:25:32.56 Exhaust.select :close @21:25:32.67 SSV.dial CBV @21:25:32.69
CBV.dial SSV @21:25:33.47 Allowing 130s for 25.0ml of remaining 925.0ml @21:25:33.48 Intake.select :open @21:25:35.64 Intake @16.1psia, vacuum from 10.0 to 13.0psi @21:25:35.65 SS.reconfigure :inhaling 1.13ml/s @21:25:35.70 SS.seek 25ml @21:26:00.18 Stroke completed -- waiting for pressure to equalize ... @21:26:00.19 SS.reconfigure :exhale @21:26:00.24 SS.hold @21:26:28.80 Intake.select :close @21:26:28.95 SSV.dial :exhaust @21:26:29.71 Exhaust.select :open @21:26:29.83 SS.seek 0ml @21:26:38.70 Exhaust.select :close @21:26:38.80 SSV.dial CBV @21:26:38.82
CBV.dial SSV @21:26:39.59 Allowing 130s for 25.0ml of remaining 900.0ml @21:26:39.60 Intake.select :open @21:26:41.77 Intake @16.1psia, vacuum from 10.0 to 13.0psi @21:26:41.78 SS.reconfigure :inhaling 1.13ml/s @21:26:41.83 SS.seek 25ml @21:27:06.16 Stroke completed -- waiting for pressure to equalize ... @21:27:06.17 SS.reconfigure :exhale @21:27:06.22 SS.hold @21:27:32.74 Intake.select :close @21:27:32.89 SSV.dial :exhaust @21:27:33.66 Exhaust.select :open @21:27:33.77 SS.seek 0ml @21:27:42.70 Exhaust.select :close @21:27:42.80 SSV.dial CBV @21:27:42.84
CBV.dial SSV @21:27:43.59 Allowing 130s for 25.0ml of remaining 875.0ml @21:27:43.60 Intake.select :open @21:27:45.76 Intake @16.1psia, vacuum from 10.0 to 13.0psi @21:27:45.78 SS.reconfigure :inhaling 1.13ml/s @21:27:45.83 SS.seek 25ml @21:28:10.33 Stroke completed -- waiting for pressure to equalize ... @21:28:10.34 SS.reconfigure :exhale @21:28:10.39 SS.hold @21:28:34.87 Intake.select :close @21:28:35.02 SSV.dial :exhaust @21:28:35.77 Exhaust.select :open @21:28:35.89 SS.seek 0ml @21:28:44.82 Exhaust.select :close @21:28:44.93 SSV.dial CBV @21:28:44.95
CBV.dial SSV @21:28:45.73 Allowing 130s for 25.0ml of remaining 850.0ml @21:28:45.74 Intake.select :open @21:28:47.90 Intake @16.1psia, vacuum from 10.0 to 13.0psi @21:28:47.91 SS.reconfigure :inhaling 1.13ml/s @21:28:47.96 SS.seek 25ml @21:29:12.22 Stroke completed -- waiting for pressure to equalize ... @21:29:12.23 SS.reconfigure :exhale @21:29:12.28 SS.hold @21:29:34.70 Intake.select :close @21:29:34.86 SSV.dial :exhaust @21:29:35.62 Exhaust.select :open @21:29:35.73 SS.seek 0ml @21:29:44.54 Exhaust.select :close @21:29:44.65 SSV.dial CBV @21:29:44.68
CBV.dial SSV @21:29:45.43 Allowing 130s for 25.0ml of remaining 825.0ml @21:29:45.44 Intake.select :open @21:29:47.61 Intake @16.2psia, vacuum from 10.0 to 13.0psi @21:29:47.63 SS.reconfigure :inhaling 1.13ml/s @21:29:47.67 SS.seek 25ml @21:30:12.41 Stroke completed -- waiting for pressure to equalize ... @21:30:12.42 SS.reconfigure :exhale @21:30:12.47 SS.hold @21:30:32.85 Intake.select :close @21:30:33.01 SSV.dial :exhaust @21:30:33.78 Exhaust.select :open @21:30:33.89 SS.seek 0ml @21:30:42.68 Exhaust.select :close @21:30:42.78 SSV.dial CBV @21:30:42.80
CBV.dial SSV @21:30:43.55 Allowing 130s for 25.0ml of remaining 800.0ml @21:30:43.56 Intake.select :open @21:30:45.73 Intake @16.1psia, vacuum from 10.0 to 13.0psi @21:30:45.74 SS.reconfigure :inhaling 1.13ml/s @21:30:45.79 SS.seek 25ml @21:31:10.28 Stroke completed -- waiting for pressure to equalize ... @21:31:10.29 SS.reconfigure :exhale @21:31:10.34 SS.hold @21:31:33.20 Intake.select :close @21:31:33.36 SSV.dial :exhaust @21:31:34.11 Exhaust.select :open @21:31:34.23 SS.seek 0ml @21:31:43.05 Exhaust.select :close @21:31:43.16 SSV.dial CBV @21:31:43.18
CBV.dial SSV @21:31:43.96 Allowing 130s for 25.0ml of remaining 775.0ml @21:31:43.97 Intake.select :open @21:31:46.14 Intake @16.1psia, vacuum from 10.0 to 13.0psi @21:31:46.15 SS.reconfigure :inhaling 1.13ml/s @21:31:46.20 SS.seek 25ml @21:32:10.91 Stroke completed -- waiting for pressure to equalize ... @21:32:10.92 SS.reconfigure :exhale @21:32:10.96 SS.hold @21:32:31.36 Intake.select :close @21:32:31.51 SSV.dial :exhaust @21:32:32.28 Exhaust.select :open @21:32:32.39 SS.seek 0ml @21:32:41.11 Exhaust.select :close @21:32:41.22 SSV.dial CBV @21:32:41.24
CBV.dial SSV @21:32:42.44 Allowing 130s for 25.0ml of remaining 750.0ml @21:32:42.45 Intake.select :open @21:32:44.62 Intake @16.1psia, vacuum from 10.0 to 13.0psi @21:32:44.63 SS.reconfigure :inhaling 1.13ml/s @21:32:44.68 SS.seek 25ml @21:33:09.34 Stroke completed -- waiting for pressure to equalize ... @21:33:09.35 SS.reconfigure :exhale @21:33:09.40 SS.hold @21:33:29.78 Intake.select :close @21:33:29.93 SSV.dial :exhaust @21:33:30.70 Exhaust.select :open @21:33:30.81 SS.seek 0ml @21:33:39.59 Exhaust.select :close @21:33:39.70 SSV.dial CBV @21:33:39.73
CBV.dial SSV @21:33:40.49 Allowing 130s for 25.0ml of remaining 725.0ml @21:33:40.50 Intake.select :open @21:33:42.66 Intake @16.1psia, vacuum from 10.0 to 13.0psi @21:33:42.67 SS.reconfigure :inhaling 1.13ml/s @21:33:42.72 SS.seek 25ml @21:34:05.68 Vacuum too high -- waiting up to 40 seconds @21:34:05.69 SS.reconfigure :exhale @21:34:05.74 SS.hold @21:34:15.92 Intake @17.7psia, vacuum from 10.0 to 13.0psi @21:34:15.94 SS.reconfigure :inhaling 0.91ml/s @21:34:15.99 SS.seek 25ml @21:34:18.47 Stroke completed -- waiting for pressure to equalize ... @21:34:18.48 SS.reconfigure :exhale @21:34:18.52 SS.hold @21:34:30.03 Intake.select :close @21:34:30.18 SSV.dial :exhaust @21:34:30.95 Exhaust.select :open @21:34:31.06 SS.seek 0ml @21:34:40.06 Exhaust.select :close @21:34:40.17 SSV.dial CBV @21:34:40.19
CBV.dial SSV @21:34:40.97 Allowing 130s for 25.0ml of remaining 700.0ml @21:34:40.98 Intake.select :open @21:34:43.15 Intake @16.2psia, vacuum from 10.0 to 13.0psi @21:34:43.16 SS.reconfigure :inhaling 0.91ml/s @21:34:43.21 SS.seek 25ml @21:35:12.47 Stroke completed -- waiting for pressure to equalize ... @21:35:12.48 SS.reconfigure :exhale @21:35:12.53 SS.hold @21:35:30.81 Intake.select :close @21:35:30.96 SSV.dial :exhaust @21:35:31.72 Exhaust.select :open @21:35:31.83 SS.seek 0ml @21:35:40.50 Exhaust.select :close @21:35:40.98 SSV.dial CBV @21:35:41.02
CBV.dial SSV @21:35:41.77 Allowing 130s for 25.0ml of remaining 675.0ml @21:35:41.78 Intake.select :open @21:35:43.94 Intake @16.1psia, vacuum from 10.0 to 13.0psi @21:35:43.95 SS.reconfigure :inhaling 0.91ml/s @21:35:44.01 SS.seek 25ml @21:36:13.61 Stroke completed -- waiting for pressure to equalize ... @21:36:13.62 SS.reconfigure :exhale @21:36:13.66 SS.hold @21:36:32.00 Intake.select :close @21:36:32.15 SSV.dial :exhaust @21:36:32.90 Exhaust.select :open @21:36:33.02 SS.seek 0ml @21:36:41.93 Exhaust.select :close @21:36:42.04 SSV.dial CBV @21:36:42.06
CBV.dial SSV @21:36:42.83 Allowing 130s for 25.0ml of remaining 650.0ml @21:36:42.84 Intake.select :open @21:36:45.00 Intake @16.1psia, vacuum from 10.0 to 13.0psi @21:36:45.01 SS.reconfigure :inhaling 0.91ml/s @21:36:45.06 SS.seek 25ml @21:37:14.40 Stroke completed -- waiting for pressure to equalize ... @21:37:14.41 SS.reconfigure :exhale @21:37:14.46 SS.hold @21:37:33.02 Intake.select :close @21:37:33.17 SSV.dial :exhaust @21:37:33.93 Exhaust.select :open @21:37:34.05 SS.seek 0ml @21:37:43.00 Exhaust.select :close @21:37:43.10 SSV.dial CBV @21:37:43.14
CBV.dial SSV @21:37:43.89 Allowing 130s for 25.0ml of remaining 625.0ml @21:37:43.90 Intake.select :open @21:37:46.06 Intake @16.2psia, vacuum from 10.0 to 13.0psi @21:37:46.08 SS.reconfigure :inhaling 0.91ml/s @21:37:46.13 SS.seek 25ml @21:38:15.62 Stroke completed -- waiting for pressure to equalize ... @21:38:15.63 SS.reconfigure :exhale @21:38:15.67 SS.hold @21:38:34.01 Intake.select :close @21:38:34.16 SSV.dial :exhaust @21:38:34.92 Exhaust.select :open @21:38:35.03 SS.seek 0ml @21:38:44.04 Exhaust.select :close @21:38:44.15 SSV.dial CBV @21:38:44.17
CBV.dial SSV @21:38:44.95 Allowing 130s for 25.0ml of remaining 600.0ml @21:38:44.96 Intake.select :open @21:38:47.13 Intake @16.1psia, vacuum from 10.0 to 13.0psi @21:38:47.14 SS.reconfigure :inhaling 0.91ml/s @21:38:47.19 SS.seek 25ml @21:39:16.85 Stroke completed -- waiting for pressure to equalize ... @21:39:16.86 SS.reconfigure :exhale @21:39:16.91 SS.hold @21:39:37.37 Intake.select :close @21:39:37.53 SSV.dial :exhaust @21:39:38.30 Exhaust.select :open @21:39:38.42 SS.seek 0ml @21:39:47.10 Exhaust.select :close @21:39:47.21 SSV.dial CBV @21:39:47.23
CBV.dial SSV @21:39:48.01 Allowing 130s for 25.0ml of remaining 575.0ml @21:39:48.02 Intake.select :open @21:39:50.19 Intake @16.1psia, vacuum from 10.0 to 13.0psi @21:39:50.20 SS.reconfigure :inhaling 0.91ml/s @21:39:50.25 SS.seek 25ml @21:40:14.86 Vacuum too high -- waiting up to 40 seconds @21:40:14.87 SS.reconfigure :exhale @21:40:14.92 SS.hold @21:40:22.49 Intake @16.9psia, vacuum from 10.0 to 13.0psi @21:40:22.86 SS.reconfigure :inhaling 0.73ml/s @21:40:22.98 SS.seek 25ml @21:40:29.79 Stroke completed -- waiting for pressure to equalize ... @21:40:29.80 SS.reconfigure :exhale @21:40:29.85 SS.hold @21:40:44.19 Intake.select :close @21:40:44.35 SSV.dial :exhaust @21:40:45.10 Exhaust.select :open @21:40:45.22 SS.seek 0ml @21:40:53.95 Exhaust.select :close @21:40:54.05 SSV.dial CBV @21:40:54.07
CBV.dial SSV @21:40:54.85 Allowing 130s for 25.0ml of remaining 550.0ml @21:40:54.86 Intake.select :open @21:40:57.03 Intake @16.1psia, vacuum from 10.0 to 13.0psi @21:40:57.04 SS.reconfigure :inhaling 0.73ml/s @21:40:57.09 SS.seek 25ml @21:41:33.43 Stroke completed -- waiting for pressure to equalize ... @21:41:33.44 SS.reconfigure :exhale @21:41:33.48 SS.hold @21:41:52.27 Intake.select :close @21:41:52.43 SSV.dial :exhaust @21:41:53.20 Exhaust.select :open @21:41:53.31 SS.seek 0ml @21:42:02.10 Exhaust.select :close @21:42:02.21 SSV.dial CBV @21:42:02.23
CBV.dial SSV @21:42:03.01 Allowing 130s for 25.0ml of remaining 525.0ml @21:42:03.02 Intake.select :open @21:42:05.19 Intake @16.2psia, vacuum from 10.0 to 13.0psi @21:42:05.20 SS.reconfigure :inhaling 0.73ml/s @21:42:05.25 SS.seek 25ml @21:42:41.74 Stroke completed -- waiting for pressure to equalize ... @21:42:41.75 SS.reconfigure :exhale @21:42:41.80 SS.hold @21:43:02.16 Intake.select :close @21:43:02.31 SSV.dial :exhaust @21:43:03.08 Exhaust.select :open @21:43:03.19 SS.seek 0ml @21:43:11.93 Exhaust.select :close @21:43:12.03 SSV.dial CBV @21:43:12.05
CBV.dial SSV @21:43:12.82 Allowing 130s for 25.0ml of remaining 500.0ml @21:43:12.83 Intake.select :open @21:43:15.00 Intake @16.1psia, vacuum from 10.0 to 13.0psi @21:43:15.01 SS.reconfigure :inhaling 0.73ml/s @21:43:15.06 SS.seek 25ml @21:43:51.38 Stroke completed -- waiting for pressure to equalize ... @21:43:51.39 SS.reconfigure :exhale @21:43:51.44 SS.hold @21:44:11.80 Intake.select :close @21:44:11.96 SSV.dial :exhaust @21:44:12.73 Exhaust.select :open @21:44:12.84 SS.seek 0ml @21:44:21.77 Exhaust.select :close @21:44:21.88 SSV.dial CBV @21:44:21.90
CBV.dial SSV @21:44:22.68 Allowing 130s for 25.0ml of remaining 475.0ml @21:44:22.69 Intake.select :open @21:44:24.86 Intake @16.2psia, vacuum from 10.0 to 13.0psi @21:44:24.87 SS.reconfigure :inhaling 0.73ml/s @21:44:24.92 SS.seek 25ml @21:45:00.81 Stroke completed -- waiting for pressure to equalize ... @21:45:00.82 SS.reconfigure :exhale @21:45:00.87 SS.hold @21:45:21.25 Intake.select :close @21:45:21.40 SSV.dial :exhaust @21:45:22.17 Exhaust.select :open @21:45:22.28 SS.seek 0ml @21:45:31.07 Exhaust.select :close @21:45:31.17 SSV.dial CBV @21:45:31.21
CBV.dial SSV @21:45:31.96 Allowing 130s for 25.0ml of remaining 450.0ml @21:45:31.97 Intake.select :open @21:45:34.13 Intake @16.3psia, vacuum from 10.0 to 13.0psi @21:45:34.15 SS.reconfigure :inhaling 0.73ml/s @21:45:34.20 SS.seek 25ml @21:46:01.63 Vacuum too high -- waiting up to 40 seconds @21:46:01.65 SS.reconfigure :exhale @21:46:01.70 SS.hold @21:46:12.00 Intake @18.4psia, vacuum from 10.0 to 13.0psi @21:46:12.02 SS.reconfigure :inhaling 0.59ml/s @21:46:12.07 SS.seek 25ml @21:46:23.29 Vacuum too high -- waiting up to 40 seconds @21:46:23.30 SS.reconfigure :exhale @21:46:23.36 SS.hold @21:46:27.59 Intake @18.3psia, vacuum from 10.0 to 13.0psi @21:46:27.61 SS.reconfigure :inhaling 0.47ml/s @21:46:27.65 SS.seek 25ml @21:46:27.84 Stroke completed -- waiting for pressure to equalize ... @21:46:27.85 SS.reconfigure :exhale @21:46:27.90 SS.hold @21:46:37.66 Intake.select :close @21:46:38.24 SSV.dial :exhaust @21:46:39.00 Exhaust.select :open @21:46:39.12 SS.seek 0ml @21:46:48.14 Exhaust.select :close @21:46:48.25 SSV.dial CBV @21:46:48.28
CBV.dial SSV @21:46:49.03 Allowing 130s for 25.0ml of remaining 425.0ml @21:46:49.05 Intake.select :open @21:46:51.21 Intake @16.2psia, vacuum from 10.0 to 13.0psi @21:46:51.23 SS.reconfigure :inhaling 0.47ml/s @21:46:51.28 SS.seek 25ml @21:47:46.51 Stroke completed -- waiting for pressure to equalize ... @21:47:46.52 SS.reconfigure :exhale @21:47:46.57 SS.hold @21:48:00.93 Intake.select :close @21:48:01.08 SSV.dial :exhaust @21:48:01.84 Exhaust.select :open @21:48:01.96 SS.seek 0ml @21:48:10.73 Exhaust.select :close @21:48:10.84 SSV.dial CBV @21:48:10.87
CBV.dial SSV @21:48:11.64 Allowing 130s for 25.0ml of remaining 400.0ml @21:48:11.65 Intake.select :open @21:48:13.82 Intake @16.4psia, vacuum from 10.0 to 13.0psi @21:48:14.23 SS.reconfigure :inhaling 0.47ml/s @21:48:14.30 SS.seek 25ml @21:49:09.95 Stroke completed -- waiting for pressure to equalize ... @21:49:09.96 SS.reconfigure :exhale @21:49:10.01 SS.hold @21:49:26.30 Intake.select :close @21:49:26.45 SSV.dial :exhaust @21:49:27.22 Exhaust.select :open @21:49:27.33 SS.seek 0ml @21:49:36.26 Exhaust.select :close @21:49:36.37 SSV.dial CBV @21:49:36.39
CBV.dial SSV @21:49:37.17 Allowing 130s for 25.0ml of remaining 375.0ml @21:49:37.18 Intake.select :open @21:49:39.35 Intake @16.4psia, vacuum from 10.0 to 13.0psi @21:49:39.36 SS.reconfigure :inhaling 0.47ml/s @21:49:39.41 SS.seek 25ml @21:50:34.95 Stroke completed -- waiting for pressure to equalize ... @21:50:34.96 SS.reconfigure :exhale @21:50:35.01 SS.hold @21:50:53.76 Intake.select :close @21:50:53.91 SSV.dial :exhaust @21:50:54.67 Exhaust.select :open @21:50:54.78 SS.seek 0ml @21:51:03.62 Exhaust.select :close @21:51:03.73 SSV.dial CBV @21:51:03.75
CBV.dial SSV @21:51:04.53 Allowing 130s for 25.0ml of remaining 350.0ml @21:51:04.54 Intake.select :open @21:51:06.71 Intake @16.4psia, vacuum from 10.0 to 13.0psi @21:51:06.72 SS.reconfigure :inhaling 0.47ml/s @21:51:06.77 SS.seek 25ml @21:52:02.45 Stroke completed -- waiting for pressure to equalize ... @21:52:02.46 SS.reconfigure :exhale @21:52:02.50 SS.hold @21:52:20.86 Intake.select :close @21:52:21.01 SSV.dial :exhaust @21:52:21.76 Exhaust.select :open @21:52:21.88 SS.seek 0ml @21:52:30.62 Exhaust.select :close @21:52:30.73 SSV.dial CBV @21:52:30.75
CBV.dial SSV @21:52:31.53 Allowing 130s for 25.0ml of remaining 325.0ml @21:52:31.54 Intake.select :open @21:52:33.70 Intake @16.4psia, vacuum from 10.0 to 13.0psi @21:52:33.72 SS.reconfigure :inhaling 0.47ml/s @21:52:33.77 SS.seek 25ml @21:53:29.15 Stroke completed -- waiting for pressure to equalize ... @21:53:29.16 SS.reconfigure :exhale @21:53:29.21 SS.hold @21:53:49.59 Intake.select :close @21:53:49.74 SSV.dial :exhaust @21:53:50.52 Exhaust.select :open @21:53:50.63 SS.seek 0ml @21:53:59.81 Exhaust.select :close @21:53:59.92 SSV.dial CBV @21:53:59.95
CBV.dial SSV @21:54:00.68 Allowing 130s for 25.0ml of remaining 300.0ml @21:54:00.69 Intake.select :open @21:54:02.86 Intake @16.4psia, vacuum from 10.0 to 13.0psi @21:54:02.87 SS.reconfigure :inhaling 0.47ml/s @21:54:02.92 SS.seek 25ml @21:54:57.55 Stroke completed -- waiting for pressure to equalize ... @21:54:57.56 SS.reconfigure :exhale @21:54:57.61 SS.hold @21:55:22.09 Intake.select :close @21:55:22.24 SSV.dial :exhaust @21:55:23.01 Exhaust.select :open @21:55:23.12 SS.seek 0ml @21:55:32.02 Exhaust.select :close @21:55:32.13 SSV.dial CBV @21:55:32.15
CBV.dial SSV @21:55:32.93 Allowing 130s for 25.0ml of remaining 275.0ml @21:55:32.94 Intake.select :open @21:55:35.11 Intake @16.3psia, vacuum from 10.0 to 13.0psi @21:55:35.12 SS.reconfigure :inhaling 0.47ml/s @21:55:35.17 SS.seek 25ml @21:56:30.49 Stroke completed -- waiting for pressure to equalize ... @21:56:30.50 SS.reconfigure :exhale @21:56:30.55 SS.hold @21:56:52.88 Intake.select :close @21:56:53.03 SSV.dial :exhaust @21:56:53.79 Exhaust.select :open @21:56:53.90 SS.seek 0ml @21:57:02.68 Exhaust.select :close @21:57:02.78 SSV.dial CBV @21:57:02.80
CBV.dial SSV @21:57:03.57 Allowing 130s for 25.0ml of remaining 250.0ml @21:57:03.58 Intake.select :open @21:57:05.75 Intake @16.5psia, vacuum from 10.0 to 13.0psi @21:57:05.76 SS.reconfigure :inhaling 0.47ml/s @21:57:05.81 SS.seek 25ml @21:58:01.69 Stroke completed -- waiting for pressure to equalize ... @21:58:01.70 SS.reconfigure :exhale @21:58:01.75 SS.hold @21:58:32.80 Intake.select :close @21:58:32.95 SSV.dial :exhaust @21:58:33.71 Exhaust.select :open @21:58:33.83 SS.seek 0ml @21:58:42.88 Exhaust.select :close @21:58:42.99 SSV.dial CBV @21:58:43.01
CBV.dial SSV @21:58:43.79 Allowing 130s for 25.0ml of remaining 225.0ml @21:58:43.80 Intake.select :open @21:58:45.96 Intake @16.2psia, vacuum from 10.0 to 13.0psi @21:58:45.97 SS.reconfigure :inhaling 0.47ml/s @21:58:46.02 SS.seek 25ml @21:59:41.28 Stroke completed -- waiting for pressure to equalize ... @21:59:41.29 SS.reconfigure :exhale @21:59:41.34 SS.hold @22:00:09.99 Intake.select :close @22:00:10.14 SSV.dial :exhaust @22:00:10.89 Exhaust.select :open @22:00:11.01 SS.seek 0ml @22:00:19.88 Exhaust.select :close @22:00:19.98 SSV.dial CBV @22:00:20.00
CBV.dial SSV @22:00:20.77 Allowing 130s for 25.0ml of remaining 200.0ml @22:00:20.78 Intake.select :open @22:00:22.95 Intake @16.5psia, vacuum from 10.0 to 13.0psi @22:00:22.96 SS.reconfigure :inhaling 0.47ml/s @22:00:23.01 SS.seek 25ml @22:01:08.12 Vacuum too high -- waiting up to 40 seconds @22:01:08.13 SS.reconfigure :exhale @22:01:08.18 SS.hold @22:01:20.96 Intake @18.7psia, vacuum from 10.0 to 13.0psi @22:01:20.97 SS.reconfigure :inhaling 0.38ml/s @22:01:21.02 SS.seek 25ml @22:01:33.21 Vacuum too high -- waiting up to 40 seconds @22:01:33.22 SS.reconfigure :exhale @22:01:33.28 SS.hold @22:01:38.22 Intake @18.3psia, vacuum from 10.0 to 13.0psi @22:01:38.24 SS.reconfigure :inhaling 0.31ml/s @22:01:38.28 SS.seek 25ml @22:01:40.41 Stroke completed -- waiting for pressure to equalize ... @22:01:40.42 SS.reconfigure :exhale @22:01:40.46 SS.hold @22:01:58.80 Intake.select :close @22:01:58.95 SSV.dial :exhaust @22:01:59.72 Exhaust.select :open @22:01:59.84 SS.seek 0ml @22:02:08.91 Exhaust.select :close @22:02:09.01 SSV.dial CBV @22:02:09.03
CBV.dial SSV @22:02:09.80 Allowing 130s for 25.0ml of remaining 175.0ml @22:02:09.81 Intake.select :open @22:02:11.97 Intake @16.3psia, vacuum from 10.0 to 13.0psi @22:02:11.99 SS.reconfigure :inhaling 0.31ml/s @22:02:12.04 SS.seek 25ml @22:03:38.39 Stroke completed -- waiting for pressure to equalize ... @22:03:38.40 SS.reconfigure :exhale @22:03:38.44 SS.hold @22:04:03.46 Intake.select :close @22:04:03.62 SSV.dial :exhaust @22:04:04.37 Exhaust.select :open @22:04:04.49 SS.seek 0ml @22:04:13.25 Exhaust.select :close @22:04:13.36 SSV.dial CBV @22:04:13.38
CBV.dial SSV @22:04:14.16 Allowing 130s for 25.0ml of remaining 150.0ml @22:04:14.17 Intake.select :open @22:04:16.33 Intake @16.3psia, vacuum from 10.0 to 13.0psi @22:04:16.34 SS.reconfigure :inhaling 0.31ml/s @22:04:16.39 SS.seek 25ml @22:05:43.29 Stroke completed -- waiting for pressure to equalize ... @22:05:43.30 SS.reconfigure :exhale @22:05:43.35 SS.hold @22:06:04.14 Intake.select :close @22:06:04.31 SSV.dial :exhaust @22:06:05.08 Exhaust.select :open @22:06:05.20 SS.seek 0ml @22:06:14.21 Exhaust.select :close @22:06:14.32 SSV.dial CBV @22:06:14.34
CBV.dial SSV @22:06:15.11 Allowing 130s for 25.0ml of remaining 125.0ml @22:06:15.12 Intake.select :open @22:06:17.28 Intake @16.6psia, vacuum from 10.0 to 13.0psi @22:06:17.29 SS.reconfigure :inhaling 0.31ml/s @22:06:17.34 SS.seek 25ml @22:07:45.79 Stroke completed -- waiting for pressure to equalize ... @22:07:45.80 SS.reconfigure :exhale @22:07:45.85 SS.hold @22:08:10.30 Intake.select :close @22:08:10.46 SSV.dial :exhaust @22:08:11.21 Exhaust.select :open @22:08:11.33 SS.seek 0ml @22:08:20.18 Exhaust.select :close @22:08:20.29 SSV.dial CBV @22:08:20.31
CBV.dial SSV @22:08:21.09 Allowing 130s for 25.0ml of remaining 100.0ml @22:08:21.10 Intake.select :open @22:08:23.26 Intake @16.6psia, vacuum from 10.0 to 13.0psi @22:08:23.28 SS.reconfigure :inhaling 0.31ml/s @22:08:23.33 SS.seek 25ml @22:09:51.66 Stroke completed -- waiting for pressure to equalize ... @22:09:51.67 SS.reconfigure :exhale @22:09:51.72 SS.hold @22:10:18.23 Intake.select :close @22:10:18.38 SSV.dial :exhaust @22:10:19.14 Exhaust.select :open @22:10:19.25 SS.seek 0ml @22:10:28.37 Exhaust.select :close @22:10:28.48 SSV.dial CBV @22:10:28.52
CBV.dial SSV @22:10:29.26 Allowing 130s for 25.0ml of remaining 75.0ml @22:10:29.27 Intake.select :open @22:10:31.43 Intake @16.6psia, vacuum from 10.0 to 13.0psi @22:10:31.45 SS.reconfigure :inhaling 0.31ml/s @22:10:31.50 SS.seek 25ml @22:11:59.30 Stroke completed -- waiting for pressure to equalize ... @22:11:59.31 SS.reconfigure :exhale @22:11:59.36 SS.hold @22:12:34.21 Intake.select :close @22:12:34.36 SSV.dial :exhaust @22:12:35.11 Exhaust.select :open @22:12:35.23 SS.seek 0ml @22:12:44.25 Exhaust.select :close @22:12:44.36 SSV.dial CBV @22:12:44.38
CBV.dial SSV @22:12:45.16 Allowing 130s for 25.0ml of remaining 50.0ml @22:12:45.17 Intake.select :open @22:12:47.34 Intake @16.5psia, vacuum from 10.0 to 13.0psi @22:12:47.35 SS.reconfigure :inhaling 0.31ml/s @22:12:47.40 SS.seek 25ml @22:14:15.48 Stroke completed -- waiting for pressure to equalize ... @22:14:15.50 SS.reconfigure :exhale @22:14:15.54 SS.hold @22:14:52.75 Intake.select :close @22:14:52.90 SSV.dial :exhaust @22:14:53.66 Exhaust.select :open @22:14:53.77 SS.seek 0ml @22:15:02.86 Exhaust.select :close @22:15:02.97 SSV.dial CBV @22:15:02.99
CBV.dial SSV @22:15:03.77 Allowing 130s for 25.0ml of remaining 25.0ml @22:15:03.78 Intake.select :open @22:15:05.95 Intake @16.4psia, vacuum from 10.0 to 13.0psi @22:15:05.96 SS.reconfigure :inhaling 0.31ml/s @22:15:06.01 SS.seek 24.99ml @22:16:33.33 Stroke completed -- waiting for pressure to equalize ... @22:16:33.34 SS.reconfigure :exhale @22:16:33.38 SS.hold @22:17:12.26 Intake.select :close @22:17:12.42 SSV.dial :exhaust @22:17:13.18 Exhaust.select :open @22:17:13.29 SS.seek 0ml @22:17:22.15 Exhaust.select :close @22:17:22.26 Sampled 1000.0ml @22:17:22.28 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) Intake.select :close @22:17:22.35 CSV.dial :waste @22:17:22.51 SSV.dial CBV,avoiding: :exhaust @22:17:22.53 CS.seek 0ml @22:17:22.56
CBV.dial SSV @22:17:22.65 CSV.dial :air @22:17:23.38 CS.seek 10ml @22:17:24.45
CTV.dial :air @22:17:25.22 delay 15 seconds @22:17:40.24 SSV.dial :spit,avoiding: :exhaust @22:17:41.98 CTV.dial CSV,avoiding: :intake @22:17:42.02
SS.seek 0ml @22:17:42.14 SSV.dial CBV,avoiding: :exhaust @22:17:47.99 SSV.dial between CBV and plug @22:17:48.47 CBV.dial between SSV and waste2 @22:17:48.86 CSV.dial between air and CTV @22:17:49.16 CS.seek 8.178ml @22:17:54.27 CTV.dial CSV,avoiding: :air @22:17:54.29
CSV.dial CTV @22:17:54.66 CS.reconfigure :pressurize @22:17:54.71 CS.seek 0ml @22:18:09.93 BubblePoint: Intake @24.2psia, Outlet @1.1psia @22:18:09.94 CBV.dial SSV @22:18:10.29 SS.reconfigure :default @22:18:10.34 SS.seek 10ml @22:18:15.95 delay 15 seconds @22:18:31.08 Evacuated: Intake @23.9psia, Outlet @23.4psia @22:18:31.09 SSV.dial :spit,avoiding: :exhaust @22:18:32.13 SS.reconfigure :exhale @22:18:32.18 SS.seek 0ml @22:18:37.43 SS.reconfigure :fast @22:18:37.48 SS.seek 5ml @22:18:40.22 SSV.dial CBV,avoiding: :exhaust @22:18:40.24
CBV.dial SSV @22:18:41.61 CTV.dial :air,avoiding: :intake @22:18:42.96 CSV.dial CTV @22:18:43.01
SS.seek 10ml @22:18:44.18 CSV.dial :air @22:18:44.61 CS.reconfigure :fast @22:18:44.66 CS.seek 0ml @22:18:45.47
SSV.dial :spit,avoiding: :exhaust @22:18:46.79 SS.reconfigure :exhale @22:18:46.83 SS.seek 0ml @22:18:51.04 CS.stop @22:18:51.87
SS.reconfigure :fast @22:18:51.91 SS.seek 5ml @22:18:54.76 SSV.dial CBV,avoiding: :exhaust @22:18:54.77
CBV.dial SSV @22:18:57.14 SSV.dial :spit,avoiding: :exhaust @22:18:58.47 SS.reconfigure :default @22:18:58.52 SS.seek 0ml @22:19:01.83 SS.stop @22:19:01.88 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @22:19:01.89 Intake.select :close @22:19:02.42 CSV.dial :waste @22:19:02.54 SSV.dial CBV,avoiding: :exhaust @22:19:02.56
CBV.dial SSV @22:19:03.21 CS.seek 0ml @22:19:03.27 CSV.dial :air @22:19:03.94
CTV.dial :air @22:19:04.03 delay 15 seconds @22:19:04.04 CS.seek 10ml @22:19:19.05
SSV.dial :spit,avoiding: :exhaust @22:19:20.37 CTV.dial CSV,avoiding: :intake @22:19:20.39
SS.reconfigure :exhale @22:19:20.49 SS.seek 0ml @22:19:20.91 SSV.dial CBV,avoiding: :exhaust @22:19:28.61 SSV.dial between CBV and plug @22:19:29.08 CBV.dial between SSV and waste2 @22:19:29.48 CSV.dial between air and CTV @22:19:29.79 CS.seek 7.449ml @22:19:36.37 CTV.dial CSV,avoiding: :air @22:19:36.39
CSV.dial CTV @22:19:36.71 CS.reconfigure :pressurize @22:19:36.76 CS.seek 0ml @22:19:52.30 BubblePoint: Intake @24.1psia, Outlet @3.4psia @22:19:52.31 CBV.dial SSV @22:19:52.64 SS.reconfigure :default @22:19:52.69 SS.seek 10ml @22:19:58.31 delay 15 seconds @22:20:13.44 Evacuated: Intake @24.0psia, Outlet @23.4psia @22:20:13.45 SSV.dial :spit,avoiding: :exhaust @22:20:14.49 SS.reconfigure :exhale @22:20:14.53 SS.seek 0ml @22:20:19.57 SS.reconfigure :fast @22:20:19.62 SS.seek 5ml @22:20:22.44 SSV.dial CBV,avoiding: :exhaust @22:20:22.46
CBV.dial SSV @22:20:23.79 CTV.dial :air,avoiding: :intake @22:20:25.14 CSV.dial CTV @22:20:25.18
SS.seek 10ml @22:20:26.40 CSV.dial :air @22:20:26.83 CS.reconfigure :fast @22:20:26.88 CS.seek 0ml @22:20:27.65
SSV.dial :spit,avoiding: :exhaust @22:20:28.97 SS.reconfigure :exhale @22:20:29.02 SS.seek 0ml @22:20:31.35 CS.stop @22:20:34.01
SS.reconfigure :fast @22:20:34.48 SS.seek 5ml @22:20:37.30 SSV.dial CBV,avoiding: :exhaust @22:20:37.32
CBV.dial SSV @22:20:39.69 SSV.dial :spit,avoiding: :exhaust @22:20:41.00 SS.reconfigure :default @22:20:41.05 SS.seek 0ml @22:20:44.32 SS.stop @22:20:44.36 Lysing: Adding 2.0ml AqMeOH @22:20:44.37 CBV.dial between CSV and waste1,avoiding: :DBV @22:20:45.89 CTV.dial CSV @22:20:47.20 CSV.dial CSR @22:20:47.99 Pulling 1ml of CSR.AqMeOH @22:20:48.00 CSR.select :AqMeOH @22:20:48.12 CS.reconfigure :default @22:20:48.16 CS.seek 1ml @22:20:51.54 delay 5 seconds @22:20:56.56 CSR.select :air @22:20:56.73 CS.seek 6ml @22:21:10.36 delay 2 seconds @22:21:12.37 CSV.dial :waste2 @22:21:12.81 CS.reconfigure :fast @22:21:12.86 CS.seek 0ml @22:21:27.48 CSV.dial CSR @22:21:27.93 Pulling 2ml of CSR.AqMeOH @22:21:27.94 CSR.select :AqMeOH @22:21:28.05 CS.reconfigure :default @22:21:28.10 CS.seek 2ml @22:21:34.11 delay 5 seconds @22:21:39.13 CSR.select :air @22:21:39.29 CS.seek 5ml @22:21:47.98 delay 2 seconds @22:21:50.00 CSV.dial CTV @22:21:50.57 CS.seek 1ml @22:22:01.93 Before lysing Collection Puck Heater at 13.9C/31.6psia @22:22:01.95 Heating Puck.from 5, :sh1 in Collection Clamp to 70C for 10:00 @22:22:01.97 CH.seek 70C,8:00 @22:24:10.56 CH.hold 10:00,70C @22:24:10.75 Begun lysing Collection Puck Heater at 68C/38.3psia @22:34:10.77 Ended lysing Collection Puck Heater at 70C/37.1psia @22:34:10.79 CS.seek 0ml @22:34:14.38 CTV.dial between spare and air,avoiding: :intake @22:34:15.41 CSV.dial :air @22:34:15.89 CS.seek 5ml @22:34:29.69 CSV.dial CTV @22:34:30.58 delay 2 seconds @22:34:32.60 CSV.dial :air @22:34:33.05 delay 2 seconds @22:34:35.06 CSV.dial CTV @22:34:35.52 delay 2 seconds @22:34:37.53 CSV.dial :air @22:34:37.99 delay 2 seconds @22:34:40.01 CS.seek 0ml @22:34:53.68 Puck @84.4psia after final lysis pressurization @22:34:53.69 Recovering Lysate into Collection syringe @22:34:53.70 CSV.dial CBV @22:34:54.70 CBV.dial CSV @22:34:55.08 CS.seek 4.999ml @22:35:08.66 delay 5 seconds @22:35:13.67 CTV.dial :air @22:35:14.20 CS.seek 6.999ml @22:35:20.06 delay 5 seconds @22:35:25.08 CSV.dial :air2 @22:35:25.52 CS.seek 10ml @22:35:33.97 CSV.dial between air and CTV,avoiding: CBV @22:35:35.13 Lysing Complete @22:35:35.20 Storing sh1 puck in Collection Clamp to tube 4 @22:35:35.38 SC.reconfigure :tight @22:35:35.42 SC.seek 4 @22:35:39.02 SC.configure :limp @22:35:39.23 Elbow.reconfigure :tight @22:35:39.28 Elbow.seek 4 @22:35:41.07 Forearm.reconfigure :tight @22:35:41.12 Forearm.seek 4 + 450 counts @22:35:42.14 SE.reconfigure :pick @22:35:42.19 SE.seek :beamBreak @22:35:44.29 SE.configure :default @22:35:44.45 Elbow.reconfigure :limp @22:35:44.50 Forearm.reconfigure :loose @22:35:44.54 Forearm.seek 4 @22:35:44.79 Elbow.hold @22:35:44.85 Elbow.reconfigure :tight @22:35:45.00 SE.seek 16.12 Pucks below Top Plate @22:35:45.47 Forearm.force -20 @22:35:45.88 Forearm.seek 4 + 301 counts @22:35:46.23 Forearm.reconfigure :tight @22:35:46.28 Forearm.seek 4 + 450 counts @22:35:46.47 SE.seek 17.12 Pucks below Top Plate @22:35:47.14 Forearm.reconfigure :loose @22:35:47.19 Forearm.seek :retract @22:35:48.13 Elbow.seek CC @22:35:49.83 Elbow.reconfigure :loose @22:35:49.93 Forearm.seek CC @22:35:50.89 Hand.close @22:35:51.44 CC.seek :open @22:35:56.16 Forearm.seek :retract @22:35:57.13 Elbow.reconfigure :tight @22:35:57.17 Elbow.seek 4 @22:35:58.94 Elbow.reconfigure :limp @22:35:58.99 Forearm.seek 4 @22:36:00.01 Elbow.hold @22:36:00.08 Elbow.reconfigure :tight @22:36:00.13 Hand.open @22:36:00.80 Forearm.force -20 @22:36:00.85 Forearm.seek 4 + 314 counts @22:36:01.14 Forearm.reconfigure :tight @22:36:01.19 Forearm.seek 4 + 450 counts @22:36:01.44 SE.seek :down @22:36:02.57
Forearm.reconfigure :loose @22:36:02.62 Forearm.seek :retract @22:36:03.02 SE.stop @22:36:03.55
Tranferring DA @22:36:03.56 Beginning samplePassC @22:36:03.57 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @22:36:04.20 Elbow.seek FlushPuck::Garage @22:36:06.05 Elbow.reconfigure :loose @22:36:06.16 Forearm.seek :garage @22:36:07.11 Hand.close @22:36:07.81 Forearm.seek :retract @22:36:08.77 Elbow.reconfigure :tight @22:36:08.81 Elbow.seek CC @22:36:11.77 Elbow.reconfigure :loose @22:36:11.87 Forearm.reconfigure :tight @22:36:11.92 Forearm.seek CC @22:36:13.14 CC.seek :closed @22:36:17.66 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.40 @22:36:17.73 Hand.open @22:36:18.37 Forearm.reconfigure :loose @22:36:18.42 Forearm.seek :retract @22:36:19.37 PSV.dial PSR @22:36:20.76 Pulling 1.5ml of PSR.flush @22:36:20.77 PSR.select :flush @22:36:20.89 PS.seek 1.5ml @22:36:36.97 delay 5 seconds @22:36:41.99 PSV.dial :waste1 @22:36:42.46 PS.seek 0ml @22:36:58.65 PSV.dial PSR @22:36:59.12 PSR.select :air @22:36:59.24 PS.seek 2.5ml @22:37:25.73 PRV.dial CBV,avoiding: :ab2 @22:37:25.75
CBV.dial PRV @22:37:27.51 PSV.dial :mixloop @22:37:28.55 CTV.dial :air,avoiding: :intake @22:37:28.72 PTV.dial :PRVmixing @22:37:30.05 PS.seek 0ml @22:37:56.40 PSV.dial :air @22:37:56.88 PS.seek 2.5ml @22:38:23.28 PSV.dial :mixloop @22:38:23.76 PS.seek 0ml @22:38:50.12 PSV.dial :air @22:38:50.60 PS.seek 2.5ml @22:39:16.93 PSV.dial :mixloop @22:39:17.41 PS.seek 0ml @22:39:43.78 Garaging FlushPuck in Collection Clamp @22:39:43.85 Elbow.reconfigure :tight @22:39:43.90 Elbow.seek CC @22:39:44.21 Elbow.reconfigure :loose @22:39:44.31 Forearm.seek CC @22:39:45.37 Hand.close @22:39:45.94 CC.seek :open @22:39:50.55 Forearm.seek :retract @22:39:51.62 Elbow.reconfigure :tight @22:39:51.67 Elbow.seek :garage @22:39:54.60 Elbow.reconfigure :loose @22:39:54.70 Forearm.reconfigure :tight @22:39:54.75 Forearm.seek :garage @22:39:55.80 Hand.open @22:39:56.49 Forearm.reconfigure :loose @22:39:56.54 Forearm.seek :retract @22:39:57.50 PSV.dial :mixloop @22:39:57.57 PTV.dial :PRVmixing @22:39:57.66 PRV.dial CBV @22:39:57.68
CBV.dial PRV @22:39:57.76 CTV.dial :air,avoiding: :intake @22:39:57.78 Loading lyfil puck from tube 5 into Collection Clamp @22:39:58.45 SC.reconfigure :tight @22:39:58.50 SC.seek 5 @22:40:02.05 SC.configure :limp @22:40:02.27 Elbow.reconfigure :tight @22:40:02.32 Elbow.seek 5 @22:40:04.23 Forearm.reconfigure :tight @22:40:04.29 Forearm.seek 5 + 450 counts @22:40:05.27 SE.reconfigure :pick @22:40:05.33 SE.seek :beamBreak @22:40:07.53 SE.configure :default @22:40:07.69 Elbow.seek 5 @22:40:07.87 Forearm.seek 5 + 300 counts @22:40:08.15 Forearm.reconfigure :loose @22:40:08.20 Hand.close @22:40:08.82 SE.seek :down @22:40:09.03
Forearm.seek :retract @22:40:09.97 Elbow.seek CC @22:40:10.68 SE.stop @22:40:11.58
Elbow.reconfigure :loose @22:40:11.68 Forearm.reconfigure :tight @22:40:11.73 Forearm.seek CC @22:40:12.94 CC.seek :closed @22:40:17.46 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.19 @22:40:17.47 Hand.open @22:40:18.12 Forearm.reconfigure :loose @22:40:18.17 Forearm.seek :retract @22:40:19.20 CTV.dial CSV,avoiding: :intake @22:40:20.54 CSV.dial CTV @22:40:20.99 PS.seek 2.5ml @22:40:47.40 CS.seek 4.499ml @22:41:02.24 delay 8 seconds @22:41:10.27 PSV.dial :air @22:41:10.73 delay 2 seconds @22:41:12.75 PSV.dial :waste2 @22:41:13.49 PS.seek 0ml @22:41:39.84 CBV.dial between PRV and waste1 @22:41:40.17 CSV.dial :air @22:41:40.61 CS.seek 10ml @22:41:55.49 CSV.dial CTV @22:41:55.95 CS.seek 0ml @22:42:22.30 CBV.dial :waste1 @22:42:22.62 delay 5 seconds @22:42:27.64 CTV.dial :air,avoiding: :intake @22:42:29.04 Storing lyfil puck in Collection Clamp to tube 4 @22:42:29.21 SC.reconfigure :tight @22:42:29.25 SC.seek 4 @22:42:32.81 SC.configure :limp @22:42:33.02 Elbow.reconfigure :tight @22:42:33.07 Elbow.seek 4 @22:42:34.85 Forearm.reconfigure :tight @22:42:34.90 Forearm.seek 4 + 450 counts @22:42:35.90 SE.reconfigure :pick @22:42:35.95 SE.seek :beamBreak @22:42:38.00 SE.configure :default @22:42:38.61 Elbow.reconfigure :limp @22:42:38.66 Forearm.reconfigure :loose @22:42:38.70 Forearm.seek 4 @22:42:38.92 Elbow.hold @22:42:38.98 Elbow.reconfigure :tight @22:42:39.08 SE.seek 17.13 Pucks below Top Plate @22:42:39.64 Forearm.force -20 @22:42:39.68 Forearm.seek 4 + 315 counts @22:42:40.03 Forearm.reconfigure :tight @22:42:40.08 Forearm.seek 4 + 450 counts @22:42:40.31 SE.seek 18.13 Pucks below Top Plate @22:42:40.97 Forearm.reconfigure :loose @22:42:41.02 Forearm.seek :retract @22:42:41.94 Elbow.seek CC @22:42:43.62 Elbow.reconfigure :loose @22:42:43.72 Forearm.seek CC @22:42:44.74 Hand.close @22:42:45.31 CC.seek :open @22:42:50.04 Forearm.seek :retract @22:42:51.07 Elbow.reconfigure :tight @22:42:51.12 Elbow.seek 4 @22:42:52.89 Elbow.reconfigure :limp @22:42:52.94 Forearm.seek 4 @22:42:53.90 Elbow.hold @22:42:53.97 Elbow.reconfigure :tight @22:42:54.01 Hand.open @22:42:55.24 Forearm.force -20 @22:42:55.28 Forearm.seek 4 + 289 counts @22:42:55.57 Forearm.reconfigure :tight @22:42:55.62 Forearm.seek 4 + 450 counts @22:42:55.87 SE.seek :down @22:42:57.02
Forearm.reconfigure :loose @22:42:57.07 Forearm.seek :retract @22:42:57.35 SE.stop @22:42:58.06
PSV.dial :air @22:42:58.81 PS.seek 2.5ml @22:43:25.09 delay 1 second @22:43:26.10 PSV.dial :mixloop @22:43:26.56 delay 1 second @22:43:27.57 PSV.dial :air @22:43:28.04 delay 1 second @22:43:29.06 PSV.dial :mixloop @22:43:29.53 delay 1 second @22:43:30.54 PSV.dial :air @22:43:31.01 delay 1 second @22:43:32.03 PS.seek 0ml @22:43:58.40 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @22:43:58.58 Elbow.seek FlushPuck::Garage @22:44:00.39 Elbow.reconfigure :loose @22:44:00.49 Forearm.seek :garage @22:44:01.45 Hand.close @22:44:02.17 Forearm.seek :retract @22:44:03.15 Elbow.reconfigure :tight @22:44:03.20 Elbow.seek PC @22:44:04.46 Elbow.reconfigure :loose @22:44:04.56 Forearm.reconfigure :tight @22:44:04.60 Forearm.seek PC @22:44:06.54 PC.seek :closed @22:44:11.21 Processing Clamp onSpring/offSpring for 16/26 samples, on/off torque 1.65 @22:44:11.28 Hand.open @22:44:11.99 Forearm.reconfigure :loose @22:44:12.04 Forearm.seek :retract @22:44:12.98 PRV.dial :air @22:44:13.45 PSV.dial :mixloop @22:44:13.98 PS.seek 0.3ml @22:44:17.83 PSV.dial PSR @22:44:19.01 Pulling 0.4ml of PSR.air @22:44:19.02 PSR.select :air @22:44:19.04 PS.seek 0.7ml @22:44:24.05 delay 3 seconds @22:44:27.07 PSR.select :air @22:44:27.09 PS.seek 2.5ml @22:44:46.34 delay 3 seconds @22:44:49.35 PSV.dial :mixloop @22:44:50.38 PTV.dial :mixing @22:44:51.13 PBV.dial :waste2 @22:44:52.09 PS.seek 0ml @22:45:18.43 Garaging FlushPuck in Processing Clamp @22:45:18.50 Elbow.reconfigure :tight @22:45:18.55 Elbow.seek PC @22:45:18.67 Elbow.reconfigure :loose @22:45:18.77 Forearm.seek PC @22:45:19.71 Hand.close @22:45:20.31 PC.seek :open @22:45:25.01 Forearm.seek :retract @22:45:26.03 Elbow.reconfigure :tight @22:45:26.07 Elbow.seek :garage @22:45:27.53 Elbow.reconfigure :loose @22:45:27.63 Forearm.reconfigure :tight @22:45:27.68 Forearm.seek :garage @22:45:29.15 Hand.open @22:45:29.99 Forearm.reconfigure :loose @22:45:30.08 Forearm.seek :retract @22:45:31.06 Loading da puck from tube 5 into Processing Clamp @22:45:31.29 SC.reconfigure :tight @22:45:31.34 SC.seek 5 @22:45:34.92 SC.configure :limp @22:45:35.15 Elbow.reconfigure :tight @22:45:35.20 Elbow.seek 5 @22:45:37.11 Forearm.reconfigure :tight @22:45:37.16 Forearm.seek 5 + 450 counts @22:45:38.36 SE.reconfigure :pick @22:45:38.42 SE.seek :beamBreak @22:45:40.67 SE.configure :default @22:45:40.83 Elbow.seek 5 @22:45:41.01 Forearm.seek 5 + 300 counts @22:45:41.23 Forearm.reconfigure :loose @22:45:41.27 Hand.close @22:45:41.95 SE.seek :down @22:45:42.14
Forearm.seek :retract @22:45:43.13 Elbow.seek PC @22:45:43.89 SE.stop @22:45:45.71
Elbow.reconfigure :loose @22:45:45.81 Forearm.reconfigure :tight @22:45:45.86 Forearm.seek PC @22:45:47.12 PC.seek :closed @22:45:51.75 Processing Clamp onSpring/offSpring for 15/25 samples, on/off torque 1.65 @22:45:51.76 Hand.open @22:45:52.49 Forearm.reconfigure :loose @22:45:52.54 Forearm.seek :retract @22:45:53.50 Heating Puck.from 5, :da in Processing Clamp to 28C for 2:00:00 @22:45:53.52 PH.seek 28C,5:00 @22:46:36.89 PH.hold 2:00:00,28C @22:46:37.39 delay 1:30 @22:48:07.43 PSR.select :air @22:48:07.45 PSV.dial PSR @22:48:08.49 Pulling 0.5ml of PSR.wash @22:48:08.50 PSR.select :wash @22:48:08.62 PS.seek 0.5ml @22:48:14.49 delay 5 seconds @22:48:19.51 PSR.select :air @22:48:19.63 PS.seek 2ml @22:48:35.77 PTV.dial :puck @22:48:36.52 PSV.dial PTV @22:48:37.28 PBV.dial :waste1 @22:48:38.67 PS.seek 1.5ml @22:48:44.55 delay 3 seconds @22:48:47.57 PBV.dial between PSV and waste1 @22:48:47.93 PS.seek 0ml @22:49:04.08 delay 5:00 @22:54:04.10 recoverPSRreagent08 @22:54:04.11 PTV.dial :puck @22:54:04.18 PBV.dial :waste1 @22:54:04.56 delay 30 seconds @22:54:34.59 PBV.dial between PSV and waste1 @22:54:34.95 PSR.select :air @22:54:34.97 PSV.dial PSR @22:54:35.74 PS.reconfigure :fast @22:54:35.78 PS.seek 2.5ml @22:54:59.54 PSV.dial PTV @22:55:00.31 delay 5 seconds @22:55:05.33 PSV.dial PSR @22:55:06.09 delay 5 seconds @22:55:11.11 PSV.dial PTV @22:55:11.87 delay 5 seconds @22:55:16.89 PSV.dial :air @22:55:17.77 PS.seek 0ml @22:55:41.11 PTV.dial :PRVmixing,via: PRV @22:55:42.43 PSV.dial :mixloop @22:55:42.89 PRV.dial :ab1 @22:55:43.64 PS.reconfigure :slow @22:55:43.69 PS.seek 0.15ml @22:55:47.29 delay 2 seconds @22:55:49.31 CBV.dial PRV @22:55:49.33
PRV.dial CBV @22:55:49.91 PS.seek 0.35ml @22:55:54.64 delay 2 seconds @22:55:56.65 CBV.dial between PRV and PSV @22:55:56.96 PRV.dial :air @22:55:57.42 PS.seek 0.9ml @22:56:09.20 PRV.dial :waste2 @22:56:09.95 PS.seek 0.5ml @22:56:18.64 addPMLReagent08 PSV.dial :air @22:56:19.11 PS.reconfigure :default @22:56:19.59 PS.seek 2ml @22:56:35.70 PSV.dial :PML @22:56:36.17 PTV.dial :mixing @22:56:36.93 PBV.dial :waste1 @22:56:37.36 PS.seek 1.85ml @22:56:39.70 PBV.dial between PSV and waste1 @22:56:40.06 PS.seek 0ml @22:56:59.76 delay 2 seconds @22:57:01.78 Beginning samplePassC @22:57:01.79 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @22:57:01.97 Elbow.reconfigure :tight @22:57:02.02 Elbow.seek FlushPuck::Garage @22:57:03.37 Elbow.reconfigure :loose @22:57:03.47 Forearm.seek :garage @22:57:04.42 Hand.close @22:57:05.16 Forearm.seek :retract @22:57:06.13 Elbow.reconfigure :tight @22:57:06.17 Elbow.seek CC @22:57:09.09 Elbow.reconfigure :loose @22:57:09.19 Forearm.reconfigure :tight @22:57:09.24 Forearm.seek CC @22:57:10.52 CC.seek :closed @22:57:15.04 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.45 @22:57:15.11 Hand.open @22:57:15.74 Forearm.reconfigure :loose @22:57:16.22 Forearm.seek :retract @22:57:17.19 PSV.dial PSR @22:57:18.24 Pulling 1.5ml of PSR.flush @22:57:18.25 PSR.select :flush @22:57:18.38 PS.seek 1.5ml @22:57:34.57 delay 5 seconds @22:57:39.59 PSV.dial :waste1 @22:57:40.06 PS.seek 0ml @22:57:56.19 PSV.dial PSR @22:57:56.66 PSR.select :air @22:57:56.78 PS.seek 2.5ml @22:58:23.20 PRV.dial CBV,avoiding: :ab2 @22:58:23.22
CBV.dial PRV @22:58:24.87 PSV.dial :mixloop @22:58:25.92 CTV.dial :air,avoiding: :intake @22:58:25.99 PTV.dial :PRVmixing @22:58:26.78 PS.seek 0ml @22:58:53.16 PSV.dial :air @22:58:53.75 PS.seek 2.5ml @22:59:20.13 PSV.dial :mixloop @22:59:20.59 PS.seek 0ml @22:59:46.91 PSV.dial :air @22:59:47.39 PS.seek 2.5ml @23:00:13.69 PSV.dial :mixloop @23:00:14.17 PS.seek 0ml @23:00:40.44 Garaging FlushPuck in Collection Clamp @23:00:40.51 Elbow.reconfigure :tight @23:00:40.56 Elbow.seek CC @23:00:40.93 Elbow.reconfigure :loose @23:00:41.02 Forearm.seek CC @23:00:42.09 Hand.close @23:00:42.67 CC.seek :open @23:00:47.27 Forearm.seek :retract @23:00:48.26 Elbow.reconfigure :tight @23:00:48.31 Elbow.seek :garage @23:00:51.19 Elbow.reconfigure :loose @23:00:51.29 Forearm.reconfigure :tight @23:00:51.34 Forearm.seek :garage @23:00:52.77 Hand.open @23:00:53.48 Forearm.reconfigure :loose @23:00:53.53 Forearm.seek :retract @23:00:54.53 delayUntil "23:05:01.77" @23:00:54.56 Whole Cell Archive sampling 1000ml @23:00:54.57 Loading wcr puck from tube 5 into Collection Clamp @23:00:54.81 SC.reconfigure :tight @23:00:54.86 SC.seek 5 @23:00:54.94 SC.configure :limp @23:00:55.17 Elbow.reconfigure :tight @23:00:55.22 Elbow.seek 5 @23:00:57.18 Forearm.reconfigure :tight @23:00:57.23 Forearm.seek 5 + 450 counts @23:00:58.23 SE.reconfigure :pick @23:00:58.29 SE.seek :beamBreak @23:01:00.68 SE.configure :default @23:01:00.85 Elbow.seek 5 @23:01:01.04 Forearm.seek 5 + 300 counts @23:01:01.53 Forearm.reconfigure :loose @23:01:01.58 Hand.close @23:01:02.18 SE.seek :down @23:01:02.38 Forearm.seek :retract @23:01:03.35 Elbow.seek CC @23:01:04.15 SE.stop @23:01:04.98 Elbow.reconfigure :loose @23:01:05.08 Forearm.reconfigure :tight @23:01:05.13 Forearm.seek CC @23:01:06.78 CC.seek :closed @23:01:11.30 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.25 @23:01:11.31 Hand.open @23:01:11.98 Forearm.reconfigure :loose @23:01:12.03 Forearm.seek :retract @23:01:13.06 collectSample 1000ml w/bubble point of 35psi @23:01:13.07 Priming 7ml @23:01:13.08 Intake.select :close @23:01:13.10 Exhaust.select :close @23:01:13.11 SS.hold @23:01:13.32 Calibrating pressure sensors @23:01:13.33 CTV.dial :air @23:01:13.35 SSV.dial :spit,avoiding: :exhaust @23:01:13.36 SS.reconfigure :fast @23:01:13.42 SS.seek 5ml @23:01:16.28 SSV.dial CBV @23:01:16.30 CBV.dial SSV @23:01:17.67 delay 3.8 seconds @23:01:21.60 Sensor limits: Intake max 102.7psia; Outlet max 94.3psia @23:01:21.61 SSV.dial :spit @23:01:22.94 SS.seek 0ml @23:01:25.68 SSV.dial CBV @23:01:27.01 CTV.dial :intake @23:01:27.77 SSV.dial CBV @23:01:27.80 CBV.dial SSV @23:01:27.88 Allowing 40s for 7.0ml of remaining 7.0ml @23:01:27.90 Intake.select :open @23:01:30.06 Intake @14.4psia, vacuum from 8.9 to 11.9psi @23:01:30.08 SS.reconfigure :inhaling 2.20ml/s @23:01:30.13 SS.seek 7ml @23:01:37.27 Stroke completed -- waiting for pressure to equalize ... @23:01:37.29 SS.reconfigure :exhale @23:01:37.33 SS.hold @23:01:51.89 Intake.select :close @23:01:52.04 SSV.dial :exhaust @23:01:52.81 Exhaust.select :open @23:01:52.92 SS.seek 0ml @23:01:57.23 Exhaust.select :close @23:01:57.34 Sampling 1000.0ml @23:01:57.36 Intake.select :close @23:01:57.37 Exhaust.select :close @23:01:57.38 SS.reconfigure :default @23:01:57.42 SS.hold @23:01:57.63 CTV.dial :intake @23:01:57.87 SSV.dial CBV @23:01:57.89 CBV.dial SSV @23:01:58.68 Allowing 130s for 25.0ml of remaining 1000.0ml @23:01:58.70 Intake.select :open @23:02:01.30 Intake @15.5psia, vacuum from 10.0 to 13.0psi @23:02:01.33 SS.reconfigure :inhaling 2.20ml/s @23:02:01.38 SS.seek 25ml @23:02:13.27 Vacuum too high -- waiting up to 40 seconds @23:02:13.28 SS.reconfigure :exhale @23:02:13.34 SS.hold @23:02:26.40 Intake @19.6psia, vacuum from 10.0 to 13.0psi @23:02:26.42 SS.reconfigure :inhaling 1.76ml/s @23:02:26.47 SS.seek 25ml @23:02:28.65 Vacuum too high -- waiting up to 40 seconds @23:02:28.66 SS.reconfigure :exhale @23:02:28.72 SS.hold @23:02:29.87 Intake @17.5psia, vacuum from 10.0 to 13.0psi @23:02:30.31 SS.reconfigure :inhaling 1.41ml/s @23:02:30.39 SS.seek 25ml @23:02:33.19 Stroke completed -- waiting for pressure to equalize ... @23:02:33.21 SS.reconfigure :exhale @23:02:33.26 SS.hold @23:03:04.02 Intake.select :close @23:03:04.18 SSV.dial :exhaust @23:03:04.95 Exhaust.select :open @23:03:05.07 SS.seek 0ml @23:03:13.91 Exhaust.select :close @23:03:14.02 SSV.dial CBV @23:03:14.05 CBV.dial SSV @23:03:14.84 Allowing 130s for 25.0ml of remaining 975.0ml @23:03:14.85 Intake.select :open @23:03:17.02 Intake @15.9psia, vacuum from 10.0 to 13.0psi @23:03:17.04 SS.reconfigure :inhaling 1.41ml/s @23:03:17.08 SS.seek 25ml @23:03:35.92 Vacuum too high -- waiting up to 40 seconds @23:03:35.94 SS.reconfigure :exhale @23:03:35.99 SS.hold @23:03:46.43 Intake @20.1psia, vacuum from 10.0 to 13.0psi @23:03:46.45 SS.reconfigure :inhaling 1.13ml/s @23:03:46.50 SS.seek 25ml @23:03:49.33 Stroke completed -- waiting for pressure to equalize ... @23:03:49.35 SS.reconfigure :exhale @23:03:49.40 SS.hold @23:04:22.10 Intake.select :close @23:04:22.26 SSV.dial :exhaust @23:04:23.04 Exhaust.select :open @23:04:23.16 SS.seek 0ml @23:04:32.28 Exhaust.select :close @23:04:32.39 SSV.dial CBV @23:04:32.41 CBV.dial SSV @23:04:33.19 Allowing 130s for 25.0ml of remaining 950.0ml @23:04:33.21 Intake.select :open @23:04:35.38 Intake @15.9psia, vacuum from 10.0 to 13.0psi @23:04:35.40 SS.reconfigure :inhaling 1.13ml/s @23:04:35.45 SS.seek 25ml @23:05:00.18 Stroke completed -- waiting for pressure to equalize ... @23:05:00.19 SS.reconfigure :exhale @23:05:00.29 SS.hold @23:05:01.78 recoverPRVreagent08 @23:05:01.79 PTV.dial :mixing @23:05:02.55 PBV.dial :waste1 @23:05:02.92 delay 30 seconds @23:05:33.00 PBV.dial between PSV and waste1 @23:05:33.37 PSV.dial :air @23:05:33.86 PS.reconfigure :fast @23:05:33.91 PS.seek 2.5ml @23:05:35.07 Intake.select :close @23:05:35.22 SSV.dial :exhaust @23:05:36.01 Exhaust.select :open @23:05:36.12 SS.seek 0ml @23:05:44.96 Exhaust.select :close @23:05:45.07 SSV.dial CBV @23:05:45.09 CBV.dial SSV @23:05:45.88 Allowing 130s for 25.0ml of remaining 925.0ml @23:05:45.89 Intake.select :open @23:05:48.06 Intake @16.0psia, vacuum from 10.0 to 13.0psi @23:05:48.08 SS.reconfigure :inhaling 1.13ml/s @23:05:48.13 SS.seek 25ml @23:05:58.09 PSV.dial :mixloop @23:05:58.56 delay 2 seconds @23:06:00.58 PSV.dial :air @23:06:01.05 delay 2 seconds @23:06:03.07 PSV.dial :mixloop @23:06:03.60 delay 2 seconds @23:06:05.62 PSV.dial :air @23:06:06.08 PS.seek 0ml @23:06:12.94 Stroke completed -- waiting for pressure to equalize ... @23:06:12.95 SS.reconfigure :exhale @23:06:13.00 SS.hold @23:06:29.59 washCycleDA #1 of 2 @23:06:29.60 PSR.select :air @23:06:29.62 PSV.dial PSR @23:06:30.95 Pulling 0.5ml of PSR.wash @23:06:30.96 PSR.select :wash @23:06:31.08 PS.reconfigure :default @23:06:31.13 PS.seek 0.5ml @23:06:37.12 delay 5 seconds @23:06:42.14 PSR.select :air @23:06:42.25 PS.seek 2ml @23:06:46.16 Intake.select :close @23:06:46.32 SSV.dial :exhaust @23:06:47.09 Exhaust.select :open @23:06:47.21 SS.seek 0ml @23:06:56.14 Exhaust.select :close @23:06:56.25 SSV.dial CBV @23:06:56.27 CBV.dial SSV @23:06:57.07 Allowing 130s for 25.0ml of remaining 900.0ml @23:06:57.08 Intake.select :open @23:06:58.34 PTV.dial :puck @23:06:59.09 PSV.dial PTV @23:06:59.25 Intake @16.0psia, vacuum from 10.0 to 13.0psi @23:06:59.27 SS.reconfigure :inhaling 1.13ml/s @23:06:59.31 SS.seek 25ml @23:06:59.84 PBV.dial :waste1 @23:07:00.28 PS.seek 1.5ml @23:07:06.19 delay 3 seconds @23:07:09.20 PBV.dial between PSV and waste1 @23:07:09.58 PS.seek 0ml @23:07:16.89 Vacuum too high -- waiting up to 40 seconds @23:07:16.91 SS.reconfigure :exhale @23:07:16.96 SS.hold @23:07:23.67 Intake @18.0psia, vacuum from 10.0 to 13.0psi @23:07:23.68 SS.reconfigure :inhaling 0.91ml/s @23:07:23.73 SS.seek 25ml @23:07:25.97 delay 2:00 @23:07:32.21 Vacuum too high -- waiting up to 40 seconds @23:07:32.22 SS.reconfigure :exhale @23:07:32.28 SS.hold @23:07:34.86 Intake @17.7psia, vacuum from 10.0 to 13.0psi @23:07:34.88 SS.reconfigure :inhaling 0.73ml/s @23:07:34.92 SS.seek 25ml @23:07:35.69 Stroke completed -- waiting for pressure to equalize ... @23:07:35.70 SS.reconfigure :exhale @23:07:35.75 SS.hold @23:07:56.19 Intake.select :close @23:07:56.35 SSV.dial :exhaust @23:07:57.13 Exhaust.select :open @23:07:57.25 SS.seek 0ml @23:08:06.08 Exhaust.select :close @23:08:06.19 SSV.dial CBV @23:08:06.22 CBV.dial SSV @23:08:07.00 Allowing 130s for 25.0ml of remaining 875.0ml @23:08:07.02 Intake.select :open @23:08:09.18 Intake @16.1psia, vacuum from 10.0 to 13.0psi @23:08:09.20 SS.reconfigure :inhaling 0.73ml/s @23:08:09.24 SS.seek 25ml @23:08:45.67 Stroke completed -- waiting for pressure to equalize ... @23:08:45.68 SS.reconfigure :exhale @23:08:45.73 SS.hold @23:09:06.58 Intake.select :close @23:09:06.74 SSV.dial :exhaust @23:09:07.51 Exhaust.select :open @23:09:07.63 SS.seek 0ml @23:09:16.67 Exhaust.select :close @23:09:16.78 SSV.dial CBV @23:09:16.80 CBV.dial SSV @23:09:17.60 Allowing 130s for 25.0ml of remaining 850.0ml @23:09:17.61 Intake.select :open @23:09:19.78 Intake @16.1psia, vacuum from 10.0 to 13.0psi @23:09:19.80 SS.reconfigure :inhaling 0.73ml/s @23:09:19.85 SS.seek 25ml @23:09:25.99 recoverPSRreagent08 @23:09:26.00 PTV.dial :puck @23:09:26.18 PBV.dial :waste1 @23:09:26.56 delay 30 seconds @23:09:55.80 Stroke completed -- waiting for pressure to equalize ... @23:09:55.81 SS.reconfigure :exhale @23:09:56.31 SS.hold @23:09:56.64 PBV.dial between PSV and waste1 @23:09:57.03 PSR.select :air @23:09:57.04 PSV.dial PSR @23:09:57.81 PS.reconfigure :fast @23:09:57.86 PS.seek 2.5ml @23:10:18.84 Intake.select :close @23:10:19.00 SSV.dial :exhaust @23:10:20.24 Exhaust.select :open @23:10:20.35 SS.seek 0ml @23:10:21.68 PSV.dial PTV @23:10:22.45 delay 5 seconds @23:10:27.47 PSV.dial PSR @23:10:28.23 delay 5 seconds @23:10:29.42 Exhaust.select :close @23:10:29.53 SSV.dial CBV @23:10:29.55 CBV.dial SSV @23:10:30.34 Allowing 130s for 25.0ml of remaining 825.0ml @23:10:30.36 Intake.select :open @23:10:32.53 Intake @16.0psia, vacuum from 10.0 to 13.0psi @23:10:32.55 SS.reconfigure :inhaling 0.73ml/s @23:10:32.60 SS.seek 25ml @23:10:33.25 PSV.dial PTV @23:10:34.01 delay 5 seconds @23:10:39.03 PSV.dial :air @23:10:39.80 PS.seek 0ml @23:11:03.22 washCycleDA #2 of 2 @23:11:03.23 PSR.select :air @23:11:03.24 PSV.dial PSR @23:11:04.59 Pulling 0.5ml of PSR.wash @23:11:04.60 PSR.select :wash @23:11:04.72 PS.reconfigure :default @23:11:04.76 PS.seek 0.5ml @23:11:08.69 Stroke completed -- waiting for pressure to equalize ... @23:11:08.71 SS.reconfigure :exhale @23:11:08.75 SS.hold @23:11:10.65 delay 5 seconds @23:11:15.72 PSR.select :air @23:11:16.32 PS.seek 2ml @23:11:27.60 Intake.select :close @23:11:27.76 SSV.dial :exhaust @23:11:28.55 Exhaust.select :open @23:11:28.66 SS.seek 0ml @23:11:32.52 PTV.dial :puck @23:11:32.60 PSV.dial PTV @23:11:33.35 PBV.dial :waste1 @23:11:33.79 PS.seek 1.5ml @23:11:37.41 Exhaust.select :close @23:11:37.51 SSV.dial CBV @23:11:37.54 CBV.dial SSV @23:11:38.33 Allowing 130s for 25.0ml of remaining 800.0ml @23:11:38.34 Intake.select :open @23:11:39.74 delay 3 seconds @23:11:40.51 Intake @16.2psia, vacuum from 10.0 to 13.0psi @23:11:40.53 SS.reconfigure :inhaling 0.73ml/s @23:11:40.58 SS.seek 25ml @23:11:42.76 PBV.dial between PSV and waste1 @23:11:43.14 PS.seek 0ml @23:11:59.31 delay 2:00 @23:12:16.89 Stroke completed -- waiting for pressure to equalize ... @23:12:16.90 SS.reconfigure :exhale @23:12:16.95 SS.hold @23:12:35.48 Intake.select :close @23:12:35.64 SSV.dial :exhaust @23:12:36.40 Exhaust.select :open @23:12:36.52 SS.seek 0ml @23:12:45.36 Exhaust.select :close @23:12:45.47 SSV.dial CBV @23:12:45.49 CBV.dial SSV @23:12:46.28 Allowing 130s for 25.0ml of remaining 775.0ml @23:12:46.29 Intake.select :open @23:12:48.46 Intake @16.3psia, vacuum from 10.0 to 13.0psi @23:12:48.48 SS.reconfigure :inhaling 0.73ml/s @23:12:48.53 SS.seek 25ml @23:13:24.96 Stroke completed -- waiting for pressure to equalize ... @23:13:24.98 SS.reconfigure :exhale @23:13:25.02 SS.hold @23:13:44.01 Intake.select :close @23:13:44.17 SSV.dial :exhaust @23:13:44.95 Exhaust.select :open @23:13:45.07 SS.seek 0ml @23:13:54.07 Exhaust.select :close @23:13:54.18 SSV.dial CBV @23:13:54.20 CBV.dial SSV @23:13:55.00 Allowing 130s for 25.0ml of remaining 750.0ml @23:13:55.02 Intake.select :open @23:13:57.19 Intake @16.1psia, vacuum from 10.0 to 13.0psi @23:13:57.21 SS.reconfigure :inhaling 0.73ml/s @23:13:57.25 SS.seek 25ml @23:13:59.33 recoverPSRreagent08 @23:13:59.35 PTV.dial :puck @23:13:59.41 PBV.dial :waste1 @23:14:00.23 delay 30 seconds @23:14:30.25 PBV.dial between PSV and waste1 @23:14:30.62 PSR.select :air @23:14:30.63 PSV.dial PSR @23:14:31.40 PS.reconfigure :fast @23:14:31.45 PS.seek 2.5ml @23:14:33.17 Stroke completed -- waiting for pressure to equalize ... @23:14:33.18 SS.reconfigure :exhale @23:14:33.23 SS.hold @23:14:53.69 Intake.select :close @23:14:53.85 SSV.dial :exhaust @23:14:55.07 Exhaust.select :open @23:14:55.19 SS.seek 0ml @23:14:55.46 PSV.dial PTV @23:14:56.35 delay 5 seconds @23:15:01.37 PSV.dial PSR @23:15:02.12 delay 5 seconds @23:15:04.21 Exhaust.select :close @23:15:04.32 SSV.dial CBV @23:15:04.36 CBV.dial SSV @23:15:05.14 Allowing 130s for 25.0ml of remaining 725.0ml @23:15:05.15 Intake.select :open @23:15:07.13 PSV.dial PTV @23:15:07.32 Intake @16.0psia, vacuum from 10.0 to 13.0psi @23:15:07.34 SS.reconfigure :inhaling 0.73ml/s @23:15:07.38 SS.seek 25ml @23:15:07.91 delay 5 seconds @23:15:12.93 PSV.dial :air @23:15:13.70 PS.seek 0ml @23:15:37.12 getPMLReagent08 @23:15:37.13 PRV.dial :air @23:15:37.58 delay 5 seconds @23:15:42.61 PTV.dial :PRVmixing @23:15:43.27 Stroke completed -- waiting for pressure to equalize ... @23:15:43.29 SS.reconfigure :exhale @23:15:43.34 SS.hold @23:15:43.99 delay 5 seconds @23:15:49.01 PRV.dial :ab2 @23:15:49.46 PTV.dial :PRVmixing @23:15:49.53 PSV.dial :mixloop @23:15:50.34 PS.reconfigure :default @23:15:50.39 PS.seek 0.3ml @23:15:54.33 delay 2 seconds @23:15:56.35 PRV.dial :air @23:15:56.88 PS.seek 0.55ml @23:16:00.24 addPMLReagent08 @23:16:00.25 PSV.dial :air @23:16:00.82 PS.seek 2ml @23:16:01.70 Intake.select :close @23:16:01.86 SSV.dial :exhaust @23:16:02.64 Exhaust.select :open @23:16:02.75 SS.seek 0ml @23:16:11.56 Exhaust.select :close @23:16:11.67 SSV.dial CBV @23:16:11.69 CBV.dial SSV @23:16:12.48 Allowing 130s for 25.0ml of remaining 700.0ml @23:16:12.49 Intake.select :open @23:16:14.66 Intake @16.2psia, vacuum from 10.0 to 13.0psi @23:16:14.68 SS.reconfigure :inhaling 0.73ml/s @23:16:14.73 SS.seek 25ml @23:16:16.52 PSV.dial :PML @23:16:16.97 PTV.dial :mixing @23:16:17.74 PBV.dial :waste1 @23:16:18.16 PS.seek 1.85ml @23:16:20.55 PBV.dial between PSV and waste1 @23:16:20.92 PS.seek 0ml @23:16:40.64 delay 2 seconds @23:16:42.66 delay 8:00 @23:16:50.99 Stroke completed -- waiting for pressure to equalize ... @23:16:51.00 SS.reconfigure :exhale @23:16:51.05 SS.hold @23:17:11.75 Intake.select :close @23:17:11.91 SSV.dial :exhaust @23:17:12.69 Exhaust.select :open @23:17:12.81 SS.seek 0ml @23:17:21.84 Exhaust.select :close @23:17:21.95 SSV.dial CBV @23:17:21.97 CBV.dial SSV @23:17:22.76 Allowing 130s for 25.0ml of remaining 675.0ml @23:17:22.77 Intake.select :open @23:17:24.94 Intake @16.1psia, vacuum from 10.0 to 13.0psi @23:17:24.96 SS.reconfigure :inhaling 0.73ml/s @23:17:25.57 SS.seek 25ml @23:18:01.35 Stroke completed -- waiting for pressure to equalize ... @23:18:01.36 SS.reconfigure :exhale @23:18:01.41 SS.hold @23:18:21.83 Intake.select :close @23:18:21.98 SSV.dial :exhaust @23:18:22.77 Exhaust.select :open @23:18:22.88 SS.seek 0ml @23:18:31.69 Exhaust.select :close @23:18:32.27 SSV.dial CBV @23:18:32.29 CBV.dial SSV @23:18:33.09 Allowing 130s for 25.0ml of remaining 650.0ml @23:18:33.10 Intake.select :open @23:18:35.27 Intake @16.2psia, vacuum from 10.0 to 13.0psi @23:18:35.29 SS.reconfigure :inhaling 0.73ml/s @23:18:35.34 SS.seek 25ml @23:19:11.64 Stroke completed -- waiting for pressure to equalize ... @23:19:11.66 SS.reconfigure :exhale @23:19:11.71 SS.hold @23:19:32.58 Intake.select :close @23:19:32.74 SSV.dial :exhaust @23:19:33.51 Exhaust.select :open @23:19:33.63 SS.seek 0ml @23:19:42.47 Exhaust.select :close @23:19:42.58 SSV.dial CBV @23:19:42.61 CBV.dial SSV @23:19:43.40 Allowing 130s for 25.0ml of remaining 625.0ml @23:19:43.41 Intake.select :open @23:19:45.58 Intake @16.3psia, vacuum from 10.0 to 13.0psi @23:19:45.60 SS.reconfigure :inhaling 0.73ml/s @23:19:45.65 SS.seek 25ml @23:20:22.00 Stroke completed -- waiting for pressure to equalize ... @23:20:22.02 SS.reconfigure :exhale @23:20:22.06 SS.hold @23:20:44.60 Intake.select :close @23:20:44.76 SSV.dial :exhaust @23:20:45.54 Exhaust.select :open @23:20:45.65 SS.seek 0ml @23:20:54.30 Exhaust.select :close @23:20:54.41 SSV.dial CBV @23:20:54.46 CBV.dial SSV @23:20:55.21 Allowing 130s for 25.0ml of remaining 600.0ml @23:20:55.22 Intake.select :open @23:20:57.39 Intake @16.1psia, vacuum from 10.0 to 13.0psi @23:20:57.41 SS.reconfigure :inhaling 0.73ml/s @23:20:57.46 SS.seek 25ml @23:21:33.85 Stroke completed -- waiting for pressure to equalize ... @23:21:33.86 SS.reconfigure :exhale @23:21:33.91 SS.hold @23:21:56.83 Intake.select :close @23:21:56.98 SSV.dial :exhaust @23:21:57.74 Exhaust.select :open @23:21:57.86 SS.seek 0ml @23:22:06.83 Exhaust.select :close @23:22:06.94 SSV.dial CBV @23:22:06.99 CBV.dial SSV @23:22:07.74 Allowing 130s for 25.0ml of remaining 575.0ml @23:22:07.75 Intake.select :open @23:22:09.92 Intake @16.3psia, vacuum from 10.0 to 13.0psi @23:22:09.94 SS.reconfigure :inhaling 0.73ml/s @23:22:09.98 SS.seek 25ml @23:22:44.59 Vacuum too high -- waiting up to 40 seconds @23:22:44.61 SS.reconfigure :exhale @23:22:44.66 SS.hold @23:22:54.56 Intake @16.9psia, vacuum from 10.0 to 13.0psi @23:22:54.59 SS.reconfigure :inhaling 0.59ml/s @23:22:54.64 SS.seek 25ml @23:22:56.44 Stroke completed -- waiting for pressure to equalize ... @23:22:56.45 SS.reconfigure :exhale @23:22:56.50 SS.hold @23:23:12.87 Intake.select :close @23:23:13.03 SSV.dial :exhaust @23:23:13.81 Exhaust.select :open @23:23:13.93 SS.seek 0ml @23:23:22.69 Exhaust.select :close @23:23:22.81 SSV.dial CBV @23:23:22.83 CBV.dial SSV @23:23:23.63 Allowing 130s for 25.0ml of remaining 550.0ml @23:23:23.64 Intake.select :open @23:23:25.81 Intake @16.1psia, vacuum from 10.0 to 13.0psi @23:23:25.83 SS.reconfigure :inhaling 0.59ml/s @23:23:25.88 SS.seek 25ml @23:24:10.79 Stroke completed -- waiting for pressure to equalize ... @23:24:10.81 SS.reconfigure :exhale @23:24:10.85 SS.hold @23:24:31.74 Intake.select :close @23:24:31.90 SSV.dial :exhaust @23:24:32.66 Exhaust.select :open @23:24:32.78 SS.seek 0ml @23:24:41.87 Exhaust.select :close @23:24:41.98 SSV.dial CBV @23:24:42.00 CBV.dial SSV @23:24:42.68 recoverPRVreagent08 @23:24:42.69 PTV.dial :mixing @23:24:42.76 PBV.dial :waste1 @23:24:42.82 Allowing 130s for 25.0ml of remaining 525.0ml @23:24:42.85 Intake.select :open @23:24:43.14 delay 30 seconds @23:24:45.02 Intake @16.2psia, vacuum from 10.0 to 13.0psi @23:24:45.04 SS.reconfigure :inhaling 0.59ml/s @23:24:45.09 SS.seek 25ml @23:25:13.16 PBV.dial between PSV and waste1 @23:25:13.54 PSV.dial :air @23:25:14.02 PS.reconfigure :fast @23:25:14.06 PS.seek 2.5ml @23:25:29.50 Stroke completed -- waiting for pressure to equalize ... @23:25:29.51 SS.reconfigure :exhale @23:25:29.56 SS.hold @23:25:38.04 PSV.dial :mixloop @23:25:38.53 delay 2 seconds @23:25:40.54 PSV.dial :air @23:25:41.01 delay 2 seconds @23:25:43.03 PSV.dial :mixloop @23:25:43.49 delay 2 seconds @23:25:45.51 PSV.dial :air @23:25:45.99 PS.seek 0ml @23:25:50.04 Intake.select :close @23:25:50.20 SSV.dial :exhaust @23:25:50.97 Exhaust.select :open @23:25:51.09 SS.seek 0ml @23:25:59.89 Exhaust.select :close @23:26:00.00 SSV.dial CBV @23:26:00.02 CBV.dial SSV @23:26:00.81 Allowing 130s for 25.0ml of remaining 500.0ml @23:26:00.83 Intake.select :open @23:26:03.00 Intake @16.3psia, vacuum from 10.0 to 13.0psi @23:26:03.02 SS.reconfigure :inhaling 0.59ml/s @23:26:03.06 SS.seek 25ml @23:26:09.61 washCycleDA #1 of 3 @23:26:09.62 PSR.select :air @23:26:09.63 PSV.dial PSR @23:26:10.96 Pulling 0.5ml of PSR.wash @23:26:10.97 PSR.select :wash @23:26:11.09 PS.reconfigure :default @23:26:11.14 PS.seek 0.5ml @23:26:17.60 delay 5 seconds @23:26:22.62 PSR.select :air @23:26:22.75 PS.seek 2ml @23:26:38.91 PTV.dial :puck @23:26:39.67 PSV.dial PTV @23:26:40.43 PBV.dial :waste1 @23:26:40.87 PS.seek 1.5ml @23:26:46.91 delay 3 seconds @23:26:47.78 Stroke completed -- waiting for pressure to equalize ... @23:26:47.79 SS.reconfigure :exhale @23:26:47.84 SS.hold @23:26:49.93 PBV.dial between PSV and waste1 @23:26:50.31 PS.seek 0ml @23:27:06.87 delay 2:00 @23:27:11.02 Intake.select :close @23:27:11.18 SSV.dial :exhaust @23:27:11.97 Exhaust.select :open @23:27:12.08 SS.seek 0ml @23:27:21.14 Exhaust.select :close @23:27:21.25 SSV.dial CBV @23:27:21.27 CBV.dial SSV @23:27:22.06 Allowing 130s for 25.0ml of remaining 475.0ml @23:27:22.07 Intake.select :open @23:27:24.24 Intake @16.3psia, vacuum from 10.0 to 13.0psi @23:27:24.26 SS.reconfigure :inhaling 0.59ml/s @23:27:24.31 SS.seek 25ml @23:28:03.24 Vacuum too high -- waiting up to 40 seconds @23:28:03.26 SS.reconfigure :exhale @23:28:03.31 SS.hold @23:28:12.38 Intake @16.9psia, vacuum from 10.0 to 13.0psi @23:28:12.46 SS.reconfigure :inhaling 0.47ml/s @23:28:12.51 SS.seek 25ml @23:28:19.35 Stroke completed -- waiting for pressure to equalize ... @23:28:19.37 SS.reconfigure :exhale @23:28:19.42 SS.hold @23:28:35.77 Intake.select :close @23:28:35.93 SSV.dial :exhaust @23:28:36.70 Exhaust.select :open @23:28:36.82 SS.seek 0ml @23:28:45.62 Exhaust.select :close @23:28:45.73 SSV.dial CBV @23:28:45.76 CBV.dial SSV @23:28:47.01 Allowing 130s for 25.0ml of remaining 450.0ml @23:28:47.03 Intake.select :open @23:28:49.19 Intake @16.3psia, vacuum from 10.0 to 13.0psi @23:28:49.21 SS.reconfigure :inhaling 0.47ml/s @23:28:49.26 SS.seek 25ml @23:29:06.90 recoverPSRreagent08 @23:29:06.91 PTV.dial :puck @23:29:06.99 PBV.dial :waste1 @23:29:07.37 delay 30 seconds @23:29:37.39 PBV.dial between PSV and waste1 @23:29:37.76 PSR.select :air @23:29:37.78 PSV.dial PSR @23:29:38.67 PS.reconfigure :fast @23:29:38.72 PS.seek 2.5ml @23:29:44.87 Stroke completed -- waiting for pressure to equalize ... @23:29:44.88 SS.reconfigure :exhale @23:29:44.93 SS.hold @23:30:02.66 PSV.dial PTV @23:30:03.41 delay 5 seconds @23:30:05.88 Intake.select :close @23:30:06.04 SSV.dial :exhaust @23:30:06.82 Exhaust.select :open @23:30:06.94 SS.seek 0ml @23:30:08.43 PSV.dial PSR @23:30:09.20 delay 5 seconds @23:30:14.22 PSV.dial PTV @23:30:14.98 delay 5 seconds @23:30:15.79 Exhaust.select :close @23:30:15.90 SSV.dial CBV @23:30:15.94 CBV.dial SSV @23:30:16.70 Allowing 130s for 25.0ml of remaining 425.0ml @23:30:16.71 Intake.select :open @23:30:18.88 Intake @16.4psia, vacuum from 10.0 to 13.0psi @23:30:18.90 SS.reconfigure :inhaling 0.47ml/s @23:30:18.95 SS.seek 25ml @23:30:19.99 PSV.dial :air @23:30:20.76 PS.seek 0ml @23:30:44.10 washCycleDA #2 of 3 @23:30:44.11 PSR.select :air @23:30:44.13 PSV.dial PSR @23:30:45.46 Pulling 0.5ml of PSR.wash @23:30:45.47 PSR.select :wash @23:30:45.58 PS.reconfigure :default @23:30:45.63 PS.seek 0.5ml @23:30:51.63 delay 5 seconds @23:30:56.65 PSR.select :air @23:30:56.77 PS.seek 2ml @23:31:13.06 PTV.dial :puck @23:31:13.14 PSV.dial PTV @23:31:14.02 PBV.dial :waste1 @23:31:14.44 PS.seek 1.5ml @23:31:14.52 Stroke completed -- waiting for pressure to equalize ... @23:31:14.54 SS.reconfigure :exhale @23:31:14.58 SS.hold @23:31:20.39 delay 3 seconds @23:31:23.42 PBV.dial between PSV and waste1 @23:31:23.78 PS.seek 0ml @23:31:39.25 Intake.select :close @23:31:39.40 SSV.dial :exhaust @23:31:40.14 delay 2:00 @23:31:40.18 Exhaust.select :open @23:31:40.30 SS.seek 0ml @23:31:49.12 Exhaust.select :close @23:31:49.23 SSV.dial CBV @23:31:49.25 CBV.dial SSV @23:31:50.04 Allowing 130s for 25.0ml of remaining 400.0ml @23:31:50.05 Intake.select :open @23:31:52.22 Intake @16.4psia, vacuum from 10.0 to 13.0psi @23:31:52.24 SS.reconfigure :inhaling 0.47ml/s @23:31:52.76 SS.seek 25ml @23:32:48.27 Stroke completed -- waiting for pressure to equalize ... @23:32:48.28 SS.reconfigure :exhale @23:32:48.33 SS.hold @23:33:12.88 Intake.select :close @23:33:13.04 SSV.dial :exhaust @23:33:14.28 Exhaust.select :open @23:33:14.40 SS.seek 0ml @23:33:23.47 Exhaust.select :close @23:33:23.58 SSV.dial CBV @23:33:23.63 CBV.dial SSV @23:33:24.35 Allowing 130s for 25.0ml of remaining 375.0ml @23:33:24.36 Intake.select :open @23:33:26.53 Intake @16.4psia, vacuum from 10.0 to 13.0psi @23:33:26.55 SS.reconfigure :inhaling 0.47ml/s @23:33:26.60 SS.seek 25ml @23:33:40.17 recoverPSRreagent08 @23:33:40.18 PTV.dial :puck @23:33:40.25 PBV.dial :waste1 @23:33:40.63 delay 30 seconds @23:34:10.65 PBV.dial between PSV and waste1 @23:34:11.03 PSR.select :air @23:34:11.05 PSV.dial PSR @23:34:11.80 PS.reconfigure :fast @23:34:11.86 PS.seek 2.5ml @23:34:21.58 Stroke completed -- waiting for pressure to equalize ... @23:34:21.59 SS.reconfigure :exhale @23:34:21.64 SS.hold @23:34:35.75 PSV.dial PTV @23:34:36.52 delay 5 seconds @23:34:41.53 PSV.dial PSR @23:34:42.30 delay 5 seconds @23:34:47.31 PSV.dial PTV @23:34:48.08 delay 5 seconds @23:34:50.33 Intake.select :close @23:34:50.49 SSV.dial :exhaust @23:34:51.26 Exhaust.select :open @23:34:51.37 SS.seek 0ml @23:34:53.10 PSV.dial :air @23:34:53.87 PS.seek 0ml @23:35:00.19 Exhaust.select :close @23:35:00.31 SSV.dial CBV @23:35:00.33 CBV.dial SSV @23:35:01.11 Allowing 130s for 25.0ml of remaining 350.0ml @23:35:01.13 Intake.select :open @23:35:03.30 Intake @16.4psia, vacuum from 10.0 to 13.0psi @23:35:03.31 SS.reconfigure :inhaling 0.47ml/s @23:35:03.36 SS.seek 25ml @23:35:17.28 washCycleDA #3 of 3 @23:35:17.29 PSR.select :air @23:35:17.31 PSV.dial PSR @23:35:18.64 Pulling 0.5ml of PSR.wash @23:35:18.65 PSR.select :wash @23:35:18.76 PS.reconfigure :default @23:35:18.81 PS.seek 0.5ml @23:35:24.84 delay 5 seconds @23:35:29.86 PSR.select :air @23:35:29.98 PS.seek 2ml @23:35:46.15 PTV.dial :puck @23:35:46.23 PSV.dial PTV @23:35:47.11 PBV.dial :waste1 @23:35:47.36 Vacuum too high -- waiting up to 40 seconds @23:35:47.37 SS.reconfigure :exhale @23:35:47.92 SS.hold @23:35:48.08 PS.seek 1.5ml @23:35:53.97 delay 3 seconds @23:35:56.98 PBV.dial between PSV and waste1 @23:35:57.36 PS.seek 0ml @23:35:59.17 Intake @17.3psia, vacuum from 10.0 to 13.0psi @23:35:59.19 SS.reconfigure :inhaling 0.38ml/s @23:35:59.23 SS.seek 25ml @23:36:13.69 delay 2:00 @23:36:13.96 Stroke completed -- waiting for pressure to equalize ... @23:36:13.97 SS.reconfigure :exhale @23:36:14.02 SS.hold @23:36:32.60 Intake.select :close @23:36:32.76 SSV.dial :exhaust @23:36:33.52 Exhaust.select :open @23:36:33.64 SS.seek 0ml @23:36:42.41 Exhaust.select :close @23:36:42.52 SSV.dial CBV @23:36:42.55 CBV.dial SSV @23:36:43.33 Allowing 130s for 25.0ml of remaining 325.0ml @23:36:43.34 Intake.select :open @23:36:45.51 Intake @16.5psia, vacuum from 10.0 to 13.0psi @23:36:45.53 SS.reconfigure :inhaling 0.38ml/s @23:36:45.58 SS.seek 25ml @23:37:54.20 Stroke completed -- waiting for pressure to equalize ... @23:37:54.21 SS.reconfigure :exhale @23:37:54.26 SS.hold @23:38:13.71 recoverPSRreagent08 @23:38:13.72 PTV.dial :puck @23:38:13.79 PBV.dial :waste1 @23:38:14.17 delay 30 seconds @23:38:21.13 Intake.select :close @23:38:21.29 SSV.dial :exhaust @23:38:22.06 Exhaust.select :open @23:38:22.66 SS.seek 0ml @23:38:31.55 Exhaust.select :close @23:38:31.66 SSV.dial CBV @23:38:31.68 CBV.dial SSV @23:38:32.47 Allowing 130s for 25.0ml of remaining 300.0ml @23:38:32.48 Intake.select :open @23:38:34.65 Intake @16.5psia, vacuum from 10.0 to 13.0psi @23:38:34.67 SS.reconfigure :inhaling 0.38ml/s @23:38:34.72 SS.seek 25ml @23:38:44.19 PBV.dial between PSV and waste1 @23:38:44.57 PSR.select :air @23:38:44.59 PSV.dial PSR @23:38:45.35 PS.reconfigure :fast @23:38:45.40 PS.seek 2.5ml @23:39:09.34 PSV.dial PTV @23:39:10.11 delay 5 seconds @23:39:15.13 PSV.dial PSR @23:39:15.89 delay 5 seconds @23:39:20.91 PSV.dial PTV @23:39:21.67 delay 5 seconds @23:39:26.69 PSV.dial :air @23:39:27.60 PS.seek 0ml @23:39:43.08 Stroke completed -- waiting for pressure to equalize ... @23:39:43.09 SS.reconfigure :exhale @23:39:43.14 SS.hold @23:39:50.93 Gate.power :camera,:ON @23:39:50.95 Mixing DA Substrates PSR.select :air @23:39:51.42 PSV.dial PSR @23:39:53.01 Pulling 0.25ml of PSR.sub1 @23:39:53.03 PSR.select :sub1 @23:39:53.34 PS.reconfigure :default @23:39:53.46 PS.seek 0.25ml @23:39:56.84 delay 5 seconds @23:40:01.87 Pulling 0.25ml of PSR.sub2 @23:40:01.88 PSR.select :sub2 @23:40:01.99 PS.seek 0.5ml @23:40:05.56 delay 5 seconds @23:40:10.57 PSR.select :air @23:40:10.69 PS.seek 2ml @23:40:14.17 Intake.select :close @23:40:14.33 SSV.dial :exhaust @23:40:15.11 Exhaust.select :open @23:40:15.23 SS.seek 0ml @23:40:24.11 Exhaust.select :close @23:40:24.22 SSV.dial CBV @23:40:24.24 CBV.dial SSV @23:40:25.04 Allowing 130s for 25.0ml of remaining 275.0ml @23:40:25.06 Intake.select :open @23:40:26.90 PTV.dial :puck @23:40:26.98 PSV.dial PTV @23:40:27.23 Intake @16.5psia, vacuum from 10.0 to 13.0psi @23:40:27.25 SS.reconfigure :inhaling 0.38ml/s @23:40:27.30 SS.seek 25ml @23:40:27.76 PBV.dial :waste1 @23:40:28.21 PS.seek 1.5ml @23:40:34.65 delay 3 seconds @23:40:37.67 PBV.dial between PSV and waste1 @23:40:38.07 PS.seek 0ml @23:40:54.21 delay 10 seconds @23:41:04.24 recoverPSRreagent08 @23:41:04.25 PTV.dial :puck @23:41:04.32 PBV.dial :waste1 @23:41:04.70 delay 30 seconds @23:41:34.73 PBV.dial between PSV and waste1 @23:41:35.11 PSR.select :air @23:41:35.13 PSV.dial PSR @23:41:35.89 PS.reconfigure :fast @23:41:35.94 PS.seek 2.5ml @23:41:36.25 Stroke completed -- waiting for pressure to equalize ... @23:41:36.26 SS.reconfigure :exhale @23:41:36.31 SS.hold @23:42:00.05 PSV.dial PTV @23:42:00.82 delay 5 seconds @23:42:05.84 PSV.dial PSR @23:42:06.60 delay 5 seconds @23:42:11.53 Intake.select :close @23:42:11.62 PSV.dial PTV @23:42:11.72 SSV.dial :exhaust @23:42:12.40 delay 5 seconds @23:42:12.51 Exhaust.select :open @23:42:12.63 SS.seek 0ml @23:42:17.42 PSV.dial :air @23:42:18.33 PS.seek 0ml @23:42:21.73 Exhaust.select :close @23:42:21.84 SSV.dial CBV @23:42:21.87 CBV.dial SSV @23:42:22.66 Allowing 130s for 25.0ml of remaining 250.0ml @23:42:22.67 Intake.select :open @23:42:24.84 Intake @16.5psia, vacuum from 10.0 to 13.0psi @23:42:24.86 SS.reconfigure :inhaling 0.38ml/s @23:42:24.91 SS.seek 25ml @23:42:42.28 Additional air clear @23:42:42.29 PSR.select :air @23:42:42.30 PSV.dial PSR @23:42:43.66 Pulling 2ml of PSR.air @23:42:43.67 PSR.select :air @23:42:43.70 PS.reconfigure :default @23:42:43.74 PS.seek 2ml @23:43:04.99 delay 5 seconds @23:43:10.01 PTV.dial :puck @23:43:10.10 PSV.dial PTV @23:43:10.88 PBV.dial :waste1 @23:43:11.31 PS.seek 1.5ml @23:43:17.30 delay 3 seconds @23:43:20.32 PBV.dial between PSV and waste1 @23:43:20.70 delay 1 second @23:43:21.71 PSV.dial :waste1 @23:43:22.79 PS.seek 0ml @23:43:32.63 Vacuum too high -- waiting up to 40 seconds @23:43:33.13 SS.reconfigure :exhale @23:43:33.19 SS.hold @23:43:39.20 Processing Puck Heater at 28.1C before imaging @23:43:39.21 PH.coast @23:43:39.32 Imaging da puck in Processing Clamp while storing it to tube 4 @23:43:39.49 SC.reconfigure :tight @23:43:39.55 SC.seek 4 @23:43:43.11 SC.configure :limp @23:43:43.33 Elbow.reconfigure :tight @23:43:43.38 Elbow.seek 4 @23:43:45.16 Forearm.reconfigure :tight @23:43:45.21 Forearm.seek 4 + 450 counts @23:43:46.55 SE.reconfigure :pick @23:43:46.61 SE.seek :beamBreak @23:43:48.60 SE.configure :default @23:43:48.77 Elbow.reconfigure :limp @23:43:48.82 Forearm.reconfigure :loose @23:43:48.86 Forearm.seek 4 @23:43:49.09 Elbow.hold @23:43:49.16 Elbow.reconfigure :tight @23:43:49.27 SE.seek 18.13 Pucks below Top Plate @23:43:49.75 Forearm.force -20 @23:43:49.79 Forearm.seek 4 + 314 counts @23:43:50.07 Forearm.reconfigure :tight @23:43:50.15 Forearm.seek 4 + 450 counts @23:43:50.39 Intake @17.5psia, vacuum from 10.0 to 13.0psi @23:43:50.41 SE.seek 19.13 Pucks below Top Plate @23:43:50.44 SS.reconfigure :inhaling 0.31ml/s @23:43:50.53 SS.seek 25ml @23:43:51.00 Forearm.reconfigure :loose @23:43:51.05 Forearm.seek :retract @23:43:51.21 Stroke completed -- waiting for pressure to equalize ... @23:43:51.23 SS.reconfigure :exhale @23:43:51.28 SS.hold @23:43:51.98 Elbow.seek PC @23:43:54.84 Elbow.reconfigure :loose @23:43:54.97 Forearm.seek PC @23:43:55.97 Hand.close @23:43:56.76 PC.seek :open @23:44:01.61 Forearm.seek :retract @23:44:02.65 Elbow.reconfigure :tight @23:44:02.70 Elbow.seek 4 @23:44:05.26 Forearm.reconfigure :tight @23:44:05.30 Forearm.seek 4 + 450 counts @23:44:06.41 Hand.open @23:44:07.07 SE.seek 18.78 Pucks below Top Plate @23:44:07.29 SE.reconfigure :seal @23:44:07.34 SE.seek 17.81 Pucks below Top Plate @23:44:07.87 Lights, camera, ACTION! @23:44:07.90 PuckCamera: snap -bin=2,2 -auto /var/log/esp/da23dec0523h1000ml.tif @23:44:10.23 Intake.select :close @23:44:10.40 SSV.dial :exhaust @23:44:11.18 Exhaust.select :open @23:44:11.86 SS.seek 0ml @23:44:20.95 Exhaust.select :close @23:44:21.06 SSV.dial CBV @23:44:21.08 CBV.dial SSV @23:44:21.88 Allowing 130s for 25.0ml of remaining 225.0ml @23:44:21.90 Intake.select :open @23:44:24.07 Intake @16.5psia, vacuum from 10.0 to 13.0psi @23:44:24.09 SS.reconfigure :inhaling 0.31ml/s @23:44:24.14 SS.seek 25ml @23:44:27.27 SX HX9/16 (USB:17): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 16.081 seconds /var/log/esp/da23dec0523h1000ml.tif: TIFF Upload Complete @23:44:27.29 PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da23dec0523h1000ml15s.tif @23:44:27.40 Waiting >20 seconds for da to relinquish FlushPuck @23:44:42.95 SX HX9/16 (USB:17): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15 seconds /var/log/esp/midres/da23dec0523h1000ml15s.tif: TIFF Upload Complete @23:44:42.98 PuckCamera: snap -bin=2,2 20.0 /var/log/esp/midres/da23dec0523h1000ml20s.tif @23:45:03.59 SX HX9/16 (USB:17): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 20 seconds /var/log/esp/midres/da23dec0523h1000ml20s.tif: TIFF Upload Complete @23:45:03.61 Gate.power :camera,:OFF @23:45:03.63 DAfans.email "16.081 sec auto-exposure",Subject: "da23dec0523h1000ml images!" @23:45:04.07 Elbow.seek 4 @23:45:04.28 Forearm.seek 4 + 450 counts @23:45:04.49 SE.reconfigure :gently @23:45:04.54 SE.seek 18.53 Pucks below Top Plate @23:45:05.31 Elbow.reconfigure :limp @23:45:05.36 Forearm.reconfigure :loose @23:45:05.41 Forearm.seek 4 @23:45:05.59 Elbow.hold @23:45:05.67 Elbow.reconfigure :tight @23:45:05.73 SE.reconfigure :default @23:45:05.78 SE.seek 18.71 Pucks below Top Plate @23:45:06.26 Forearm.force -20 @23:45:06.31 Forearm.seek 4 + 316 counts @23:45:06.60 Forearm.reconfigure :tight @23:45:06.65 Forearm.seek 4 + 450 counts @23:45:06.91 SE.seek :down @23:45:08.16 Forearm.reconfigure :loose @23:45:08.22 Forearm.seek :retract @23:45:08.35 SE.stop @23:45:09.29 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @23:45:09.68 Elbow.seek FlushPuck::Garage @23:45:11.69 Elbow.reconfigure :loose @23:45:11.88 Forearm.seek :garage @23:45:12.90 Hand.close @23:45:13.77 Forearm.seek :retract @23:45:14.83 Elbow.reconfigure :tight @23:45:14.97 Elbow.seek PC @23:45:16.37 Elbow.reconfigure :loose @23:45:16.57 Forearm.reconfigure :tight @23:45:16.66 Forearm.seek PC @23:45:18.25 PC.seek :closed @23:45:23.03 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.64 @23:45:23.22 Hand.open @23:45:24.02 Forearm.reconfigure :loose @23:45:24.12 Forearm.seek :retract @23:45:25.17 PSR.select :air @23:45:25.21 PSV.dial PSR @23:45:25.73 Pulling 0.5ml of PSR.flush @23:45:25.75 PSR.select :flush @23:45:25.92 PS.seek 0.5ml @23:45:31.95 delay 5 seconds @23:45:36.98 PSR.select :air @23:45:37.17 PS.seek 2.5ml @23:45:38.79 upsync @23:45:51.74 Stroke completed -- waiting for pressure to equalize ... @23:45:51.75 SS.reconfigure :exhale @23:45:51.80 SS.hold @23:45:58.52 delay 1 second @23:45:59.59 PRV.dial :waste2,avoiding: :ab2 @23:46:01.53 PTV.dial :PRVmixing @23:46:02.88 PSV.dial :mixloop @23:46:03.93 PS.seek :empty @23:46:23.25 Intake.select :close @23:46:23.46 SSV.dial :exhaust @23:46:24.23 Exhaust.select :open @23:46:24.35 SS.seek 0ml @23:46:30.30 PSR.select :air @23:46:30.32 PSV.dial PSR @23:46:31.38 Pulling 0.5ml of PSR.flush @23:46:31.39 PSR.select :flush @23:46:31.51 PS.seek 0.5ml @23:46:33.43 Exhaust.select :close @23:46:33.54 SSV.dial CBV @23:46:33.56 CBV.dial SSV @23:46:34.35 Allowing 130s for 25.0ml of remaining 200.0ml @23:46:34.37 Intake.select :open @23:46:36.54 Intake @16.5psia, vacuum from 10.0 to 13.0psi @23:46:36.56 SS.reconfigure :inhaling 0.31ml/s @23:46:36.61 SS.seek 25ml @23:46:37.43 delay 5 seconds @23:46:42.44 PSR.select :air @23:46:42.56 PS.seek 2.5ml @23:47:03.83 delay 1 second @23:47:04.85 PBV.dial :waste1 @23:47:05.22 PTV.dial :mixing @23:47:05.97 PSV.dial :mixloop @23:47:07.04 PS.seek :empty @23:47:33.42 PSV.dial PSR @23:47:34.47 PSR.select :flush @23:47:34.59 PS.seek 1ml @23:47:45.64 PSR.select :air @23:47:45.76 PS.seek 2.5ml @23:48:01.89 PSV.dial PTV @23:48:02.66 PTV.dial :puck @23:48:03.42 PBV.dial :waste1 @23:48:03.47 Stroke completed -- waiting for pressure to equalize ... @23:48:03.50 SS.reconfigure :exhale @23:48:03.57 SS.hold @23:48:03.59 PS.seek 0ml @23:48:30.18 PSV.dial PSR @23:48:30.95 PSR.select :flush @23:48:31.08 PS.seek 0.5ml @23:48:36.95 PSR.select :air @23:48:37.07 PS.seek 2.5ml @23:48:40.40 Intake.select :close @23:48:41.01 SSV.dial :exhaust @23:48:41.77 Exhaust.select :open @23:48:41.89 SS.seek 0ml @23:48:51.00 Exhaust.select :close @23:48:51.11 SSV.dial CBV @23:48:51.13 CBV.dial SSV @23:48:51.91 Allowing 130s for 25.0ml of remaining 175.0ml @23:48:51.93 Intake.select :open @23:48:54.09 Intake @16.5psia, vacuum from 10.0 to 13.0psi @23:48:54.11 SS.reconfigure :inhaling 0.31ml/s @23:48:54.16 SS.seek 25ml @23:48:58.36 PSV.dial PTV @23:48:59.13 PBV.dial PSV @23:48:59.69 PS.seek 0ml @23:49:26.23 PSV.dial PBV @23:49:26.71 PS.seek 2.5ml @23:49:53.14 PSV.dial :waste1 @23:49:53.90 PS.seek 0ml @23:50:20.26 PSV.dial PSR @23:50:20.73 PSR.select :air @23:50:20.76 PS.seek 2.5ml @23:50:21.92 Stroke completed -- waiting for pressure to equalize ... @23:50:21.94 SS.reconfigure :exhale @23:50:22.02 SS.hold @23:50:47.11 PSV.dial PTV @23:50:47.97 PBV.dial PSV @23:50:48.05 PS.seek 0ml @23:51:01.93 SS.reconfigure :default @23:51:01.99 SS.hold @23:51:02.05 Intake.select :close @23:51:02.21 ShallowSampler::Clogged in WCR -- Filtering too Slowly after 850.0ml @23:51:02.23 SSV.dial :exhaust @23:51:02.99 Exhaust.select :open @23:51:03.10 SS.reconfigure :exhale @23:51:03.15 SS.seek 0ml @23:51:12.20 Exhaust.select :close @23:51:12.31 Sampled 850.0ml @23:51:12.33 Drying puck before fixing for 5:00 @23:51:12.34 CBV.dial :waste2b @23:51:13.49 delay 1:00 @23:51:14.45 PSV.dial PBV @23:51:14.91 PS.seek 2.5ml @23:51:41.29 PSV.dial :waste1 @23:51:42.04 PS.seek 0ml @23:52:08.45 PSV.dial PSR @23:52:08.92 PSR.select :flush @23:52:09.04 PS.seek 1ml @23:52:13.57 CSV.dial :air @23:52:14.04 CS.reconfigure :fast @23:52:14.10 CS.seek 10ml @23:52:20.14 PSR.select :air @23:52:20.26 PS.seek 2.5ml @23:52:36.45 PSV.dial PTV @23:52:37.18 PTV.dial :puck @23:52:37.26 PBV.dial :waste1 @23:52:37.83 PS.seek 0ml @23:52:38.49 Pumping wcr puck from tube 5 up to 35psi... @23:52:38.51 CBV.dial SSV @23:52:39.78 CBV.dial between CSV and waste2b @23:52:40.75 CSV.dial between air and CTV @23:52:41.08 CS.seek 6.921ml @23:52:48.81 CTV.dial CSV,avoiding: :air @23:52:48.83 CSV.dial CTV @23:52:49.72 CS.reconfigure :pressurize @23:52:49.77 CS.seek 0ml @23:53:04.17 PSV.dial PSR @23:53:05.04 PSR.select :flush @23:53:05.17 PS.seek 0.5ml @23:53:07.07 CBV.dial :waste2b @23:53:07.40 CS.reconfigure :default @23:53:07.45 CS.seek 0ml @23:53:09.02 delay 1:00 @23:53:11.07 PSR.select :air @23:53:11.19 PS.seek 2.5ml @23:53:32.51 PSV.dial PTV @23:53:33.37 PBV.dial PSV @23:53:33.94 PS.seek 0ml @23:54:00.54 PSV.dial PBV @23:54:01.00 PS.seek 2.5ml @23:54:09.05 CTV.dial :air,avoiding: :intake @23:54:10.39 Fix #1 of 2 (with RNAlater) for 2:30 @23:54:10.40 CSV.dial CSR @23:54:10.83 Pulling 2ml of CSR.RNAlater @23:54:10.85 CSR.select :RNAlater @23:54:10.97 CS.seek 2ml @23:54:16.90 delay 5 seconds @23:54:21.92 CSR.select :air @23:54:22.04 CS.reconfigure :fast @23:54:22.09 CS.seek 10ml @23:54:27.80 PSV.dial :waste1 @23:54:28.53 PS.seek 0ml @23:54:41.55 Pumping wcr puck from tube 5 up to 35psi... @23:54:41.57 CBV.dial SSV @23:54:42.82 CBV.dial between CSV and waste2b @23:54:43.80 CSV.dial between air and CTV @23:54:44.40 CS.seek 6.906ml @23:54:52.18 CTV.dial CSV,avoiding: :air @23:54:52.23 CSV.dial CTV @23:54:53.54 CS.reconfigure :pressurize @23:54:53.59 CS.seek 0ml @23:54:54.98 PSV.dial PSR @23:54:55.45 PSR.select :air @23:54:55.47 PS.seek 2.5ml @23:55:06.35 delay 2:30 @23:55:21.85 PSV.dial PTV @23:55:22.59 PBV.dial PSV @23:55:22.67 PS.seek 0ml @23:55:49.04 PSV.dial PBV @23:55:49.52 PS.seek 2.5ml @23:56:15.85 PSV.dial :waste1 @23:56:16.59 PS.seek 0ml @23:56:42.98 Garaging FlushPuck in Processing Clamp @23:56:43.05 Elbow.reconfigure :tight @23:56:43.10 Elbow.seek PC @23:56:43.22 Elbow.reconfigure :loose @23:56:43.32 Forearm.seek PC @23:56:44.30 Hand.close @23:56:44.88 PC.seek :open @23:56:50.04 Forearm.seek :retract @23:56:51.00 Elbow.reconfigure :tight @23:56:51.05 Elbow.seek :garage @23:56:52.40 Elbow.reconfigure :loose @23:56:52.50 Forearm.reconfigure :tight @23:56:52.55 Forearm.seek :garage @23:56:53.58 Hand.open @23:56:54.31 Forearm.reconfigure :loose @23:56:54.36 Forearm.seek :retract @23:57:36.38 CBV.dial :waste2b @23:57:36.73 CS.reconfigure :default @23:57:36.78 CS.seek 0ml @23:57:42.89 delay 1:00 @23:58:42.92 CTV.dial :air,avoiding: :intake @23:58:44.24 Fix #2 of 2 (with RNAlater) for 2:30 @23:58:44.26 CSV.dial CSR @23:58:44.69 Pulling 2ml of CSR.RNAlater @23:58:44.70 CSR.select :RNAlater @23:58:44.82 CS.seek 2ml @23:58:50.99 delay 5 seconds @23:58:56.01 CSR.select :air @23:58:56.12 CS.reconfigure :fast @23:58:56.17 CS.seek 10ml @23:59:15.67 Pumping wcr puck from tube 5 up to 35psi... @23:59:15.69 CBV.dial SSV @23:59:16.96 CBV.dial between CSV and waste2b @23:59:17.93 CSV.dial between air and CTV @23:59:18.54 CS.seek 6.906ml @23:59:26.36 CTV.dial CSV,avoiding: :air @23:59:26.41 CSV.dial CTV @23:59:27.73 CS.reconfigure :pressurize @23:59:27.78 CS.seek 0ml @23:59:44.54 delay 2:30 @00:02:14.56UTC06-Dec-23 CBV.dial :waste2b @00:02:14.90 CS.reconfigure :default @00:02:14.95 CS.seek 0ml @00:02:17.26 delay 1:00 @00:03:17.29 CTV.dial :air,avoiding: :intake @00:03:19.11 Storing wcr puck in Collection Clamp to tube 4 @00:03:19.53 Elbow.reconfigure :tight @00:03:19.58 Elbow.seek 4 @00:03:21.43 Forearm.reconfigure :tight @00:03:21.48 Forearm.seek 4 + 450 counts @00:03:22.50 SE.reconfigure :pick @00:03:22.56 SE.seek :beamBreak @00:03:24.47 SE.configure :default @00:03:24.64 Elbow.reconfigure :limp @00:03:24.69 Forearm.reconfigure :loose @00:03:24.74 Forearm.seek 4 @00:03:24.96 Elbow.hold @00:03:25.03 Elbow.reconfigure :tight @00:03:25.14 SE.seek 19.12 Pucks below Top Plate @00:03:25.58 Forearm.force -20 @00:03:25.62 Forearm.seek 4 + 304 counts @00:03:25.91 Forearm.reconfigure :tight @00:03:25.96 Forearm.seek 4 + 450 counts @00:03:26.16 SE.seek 20.12 Pucks below Top Plate @00:03:26.82 Forearm.reconfigure :loose @00:03:26.87 Forearm.seek :retract @00:03:27.80 Elbow.seek CC @00:03:29.40 Elbow.reconfigure :loose @00:03:29.50 Forearm.seek CC @00:03:30.96 Hand.close @00:03:31.63 CC.seek :open @00:03:36.38 Forearm.seek :retract @00:03:37.36 Elbow.reconfigure :tight @00:03:37.41 Elbow.seek 4 @00:03:39.15 Elbow.reconfigure :limp @00:03:39.20 Forearm.seek 4 @00:03:40.17 Elbow.hold @00:03:40.24 Elbow.reconfigure :tight @00:03:40.29 Hand.open @00:03:40.99 Forearm.force -20 @00:03:41.04 Forearm.seek 4 + 319 counts @00:03:41.33 Forearm.reconfigure :tight @00:03:41.38 Forearm.seek 4 + 450 counts @00:03:41.66 SE.seek :down @00:03:42.79 Forearm.reconfigure :loose @00:03:42.84 Forearm.seek :retract @00:03:43.01 SE.stop @00:03:43.77
Starting finalPurge @00:03:43.78 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @00:03:43.97 Elbow.seek FlushPuck::Garage @00:03:45.71 Elbow.reconfigure :loose @00:03:45.81 Forearm.seek :garage @00:03:46.75 Hand.close @00:03:47.93 Forearm.seek :retract @00:03:48.94 Elbow.reconfigure :tight @00:03:48.98 Elbow.seek CC @00:03:51.94 Elbow.reconfigure :loose @00:03:52.04 Forearm.reconfigure :tight @00:03:52.09 Forearm.seek CC @00:03:53.29 CC.seek :closed @00:03:57.82 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.35 @00:03:57.89 Hand.open @00:03:58.56 Forearm.reconfigure :loose @00:03:58.60 Forearm.seek :retract @00:03:59.58 Flushing the Collection Valve::Manifold @00:03:59.70 CSV.dial CSR @00:04:00.14 Pulling 4.999ml of CSR.flush @00:04:00.15 CSR.select :flush @00:04:00.26 CS.seek 5ml @00:04:13.86 delay 5 seconds @00:04:18.87 CSR.select :air @00:04:19.04 CS.reconfigure :fast @00:04:19.09 CS.seek 7.5ml @00:04:25.92 CSV.dial :waste @00:04:27.20 CS.reconfigure :default @00:04:27.25 CS.seek 0ml @00:04:47.29 Flushing lower manifold's waste1 port @00:04:47.42 CSV.dial :flush @00:04:47.88 CS.reconfigure :fast @00:04:47.92 CS.seek 5ml @00:05:00.57 delay 5 seconds @00:05:05.59 CSV.dial :air @00:05:06.09 CS.seek 7ml @00:05:11.92 CSV.dial CTV @00:05:11.94
CTV.dial CSV @00:05:13.34 CBV.dial :waste1 @00:05:14.11 CS.seek 0ml @00:05:31.04 Flushing lower manifold's waste2 port @00:05:31.16 CSV.dial :flush @00:05:31.89 CS.seek 5ml @00:05:44.54 delay 5 seconds @00:05:49.56 CSV.dial :air @00:05:50.09 CS.seek 7.001ml @00:05:55.79 CSV.dial CTV @00:05:55.82
CTV.dial CSV @00:05:56.42 CBV.dial :waste2 @00:05:57.47 CS.seek 0ml @00:06:14.04 Flushing lower manifold's waste2b port @00:06:14.16 CSV.dial :flush @00:06:14.89 CS.seek 5ml @00:06:27.51 delay 5 seconds @00:06:32.53 CSV.dial :air @00:06:33.04 CS.seek 7ml @00:06:38.51 CSV.dial CTV @00:06:38.54
CTV.dial CSV @00:06:38.98 CBV.dial :waste2b @00:06:40.03 CS.seek 0ml @00:06:56.54 Disinfecting external Intake with kill @00:06:56.66 CSV.dial CSR @00:06:57.21 Pulling 8ml of CSR.kill @00:06:57.22 CSR.select :kill @00:06:57.34 CS.seek 8ml @00:07:17.35 delay 5 seconds @00:07:22.37 CSR.select :air @00:07:22.49 CS.seek 10ml @00:07:28.20 CSV.dial CBV @00:07:28.22
CBV.dial CSV @00:07:29.46 CTV.dial :intake @00:07:30.23 Intake.select :open @00:07:30.34 CS.seek 2ml @00:07:49.23 Intake.select :close @00:07:49.33 CTV.dial :air @00:07:50.08 CS.reconfigure :default @00:07:50.12 CS.seek 0ml @00:07:56.10 CSV.dial CSR @00:07:57.10 Pulling 5ml of CSR.flush @00:07:57.11 CSR.select :flush @00:07:57.22 CS.seek 5ml @00:08:10.79 delay 5 seconds @00:08:15.81 CSR.select :air @00:08:15.93 CS.seek 10ml @00:08:29.63 CSV.dial CBV @00:08:30.64 CS.seek 0ml @00:08:57.07 Garaging FlushPuck in Collection Clamp @00:08:57.14 Elbow.reconfigure :tight @00:08:57.64 Elbow.seek CC @00:08:57.97 Elbow.reconfigure :loose @00:08:58.08 Forearm.seek CC @00:08:59.10 Hand.close @00:08:59.75 CC.seek :open @00:09:04.37 Forearm.seek :retract @00:09:05.37 Elbow.reconfigure :tight @00:09:05.42 Elbow.seek :garage @00:09:08.35 Elbow.reconfigure :loose @00:09:08.45 Forearm.reconfigure :tight @00:09:08.50 Forearm.seek :garage @00:09:09.47 Hand.open @00:09:10.18 Forearm.reconfigure :loose @00:09:10.22 Forearm.seek :retract @00:09:11.20 Completed [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] begun 21:16:44 @00:09:11.25 Asleep after completing DA_WCR_120523b assays @00:09:11.27 GoodNews.email "{1 => 33,\n 2 => 33,\n 3 => 31,\n 4 => 21,\n 5 => 12,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@00:06:04, 30% flow, 16.9C, 32% humidity, 15.1psia, 13.715V, 0.417A, 158.008Ah, 5.72W\nSampled 850.0ml",Subject: "Asleep after completing DA_WCR_120523b assays" @00:09:16.23 upsync + ESPwhich DA_WCR_120623.rb + fn=DA_WCR_120623.rb + IFS=: + set /home/esp/esp2/mission . + fullPath=/home/esp/esp2/mission/DA_WCR_120623.rb + [ -f /home/esp/esp2/mission/DA_WCR_120623.rb ] + realpath /home/esp/esp2/mission/DA_WCR_120623.rb + return 0 + scriptPath=/home/esp/esp2local/mission/DA_WCR_120623.rb + exec ruby /home/esp/esp2/lib/esp.rb /home/esp/esp2local/mission/DA_WCR_120623.rb @13:25:52.98UTC06-Dec-23
ESP2 v3.42 I2C Gateway v3.20 for core bus @13:25:55.61 Configuration for short puck jake 1/11/21 by brent@mbari.org /home/esp/esp2/type/shallow/jake/configure.rb @13:25:59.24 Polling Can every 10:00 @13:25:59.31 Cleanly exited at 13:23:11UTC06-Dec-23 @13:25:59.35 Loading /home/esp/esp2local/mission/DA_WCR_120623.rb mission @13:25:59.41 GoodNews.email "Starting DA_WCR_120623 w/pucks in tube 5\n{1 => 33,\n 2 => 33,\n 3 => 31,\n 4 => 21,\n 5 => 12,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@13:25:59, 31% flow, 16.1C, 31% humidity, 15.1psia, 13.749V, 0.260A, 161.237Ah, 3.58W",Subject: "DA_WCR_120623 begun" @13:25:59.44
Preparing to sleep until + 00:00:05 @13:25:59.49 Asleep til + 00:00:05 @13:25:59.50 Continuing at 13:26 @13:25:59.79 Gate.power :core,:OFF @13:25:59.80 Gate.power :raw,:OFF @13:25:59.81 Shutdown @13:26:04.39
Powering core bus @13:26:05.53 GoodNews.email "Then, in tube 5: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_120623.rb:14\n{1 => 33,\n 2 => 33,\n 3 => 31,\n 4 => 21,\n 5 => 12,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@13:25:59, 31% flow, 16.1C, 31% humidity, 15.1psia, 13.749V, 0.266A, 161.237Ah, 3.66W",Subject: "Asleep until 13:26" @13:26:06.89 upsync @13:26:09.71
Gate.power :raw,:ON @13:26:10.33 upsync @13:26:10.49
CS.configure :default @13:26:10.53 PS.configure :default @13:26:10.58 SS.configure :default @13:26:10.63 CC.configure :default @13:26:10.68 PC.configure :default @13:26:10.75 Forearm.configure :loose @13:26:10.80 Elbow.configure :loose @13:26:10.87 SC.configure :loose @13:26:10.92 SE.configure :default @13:26:10.96 CH.configure :default @13:26:11.00 PH.configure :default @13:26:11.04 All dwarf controllers running firmware version 3.85 @13:26:11.15 Valve.reset @13:26:11.44 Intake.select :close @13:26:11.46 Exhaust.select :close @13:26:11.47 CTV.dial :air @13:26:11.55 PSV.dial :air @13:26:11.60 CSV.dial :waste1 @13:26:11.74
Forearm.home.seek :retract @13:26:12.13 SSV.dial :spit,avoiding: :exhaust @13:26:12.49 Hand.close @13:26:12.51
Elbow.reconfigure :loose @13:26:12.62 Elbow.jog 1500 @13:26:12.83 PS.home.seek 0ml @13:26:13.02 CS.reconfigure :fast @13:26:13.06 SS.home.seek 0ml @13:26:13.15 CS.home.seek 0ml @13:26:13.23
Elbow.reconfigure :loose @13:26:13.28 Elbow.home.seek :clear @13:26:16.64 SE.home.seek :down @13:26:17.26 SC.reconfigure :tight @13:26:17.31 SC.home.seek :initial @13:26:21.04 PS.stop @13:26:36.26
SC.configure :limp @13:26:36.31 Forearm.seek :retract @13:26:36.38 Elbow.seek :hammer @13:26:37.43 Forearm.reconfigure :tight @13:26:37.48 Forearm.seek retracted @13:26:38.09 PC.home.seek :open @13:26:38.18
CC.home.seek :open @13:26:41.58 Phase [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] @13:26:41.60 Notice.email "At 13:26:41 began in tube 5: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_120623.rb:14\n{1 => 33,\n 2 => 33,\n 3 => 31,\n 4 => 21,\n 5 => 12,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@13:26:41, 31% flow, 16.1C, 31% humidity, 15.1psia, 13.695V, 0.296A, 161.242Ah, 4.06W",Subject: "Started DA_WCR_120623 phase initialPurge; da [1000, 1000] #da and wsr are run simultanelously" @13:26:41.61
Starting initialPurge @13:26:41.64 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @13:26:41.71 Hand.open @13:26:42.45 Elbow.reconfigure :tight @13:26:42.50 Elbow.seek FlushPuck::Garage @13:26:44.40 Elbow.reconfigure :loose @13:26:44.52 Forearm.reconfigure :loose @13:26:44.56 Forearm.seek :garage @13:26:45.56 Hand.close @13:26:45.98 upsync @13:26:46.34
Forearm.seek :retract @13:26:47.37 Elbow.reconfigure :tight @13:26:47.42 Elbow.seek CC @13:26:50.36 Elbow.reconfigure :loose @13:26:50.47 Forearm.reconfigure :tight @13:26:50.52 Forearm.seek CC @13:26:51.79 CC.seek :closed @13:26:56.37 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.39 @13:26:56.44 Hand.open @13:26:57.14 Forearm.reconfigure :loose @13:26:57.19 Forearm.seek :retract @13:26:58.14 Flushing the Collection Valve::Manifold @13:26:58.27 CSV.dial CSR @13:26:59.56 Pulling 5ml of CSR.flush @13:26:59.57 CSR.select :flush @13:26:59.69 CS.reconfigure :default @13:27:00.15 CS.seek 5ml @13:27:13.78 delay 5 seconds @13:27:18.80 CSR.select :air @13:27:18.97 CS.reconfigure :fast @13:27:19.02 CS.seek 7.5ml @13:27:25.75 CSV.dial :waste @13:27:27.03 CS.reconfigure :default @13:27:27.08 CS.seek 0ml @13:27:47.18 Purging external Intake @13:27:47.31 CSV.dial :flush @13:27:47.77 CS.reconfigure :fast @13:27:47.82 CS.seek 10ml @13:28:12.18 delay 5 seconds @13:28:17.21 CSV.dial CBV @13:28:17.24
CBV.dial CSV @13:28:18.00 CTV.dial :intake @13:28:18.80 Intake.select :open @13:28:18.90 CS.seek 0ml @13:28:42.46 Intake.select :close @13:28:42.57 CTV.dial :air @13:28:43.34 Flushing upper manifold @13:28:43.46 CSV.dial :flush @13:28:44.19 CS.seek 5ml @13:28:57.03 delay 5 seconds @13:29:02.05 CSV.dial :air @13:29:02.55 CS.seek 7ml @13:29:08.09 CSV.dial CBV @13:29:08.11
CBV.dial CSV @13:29:09.15 CTV.dial :air @13:29:09.17 CS.seek 0ml @13:29:25.65 delay 7 seconds @13:29:32.68 CS.seek 3ml @13:29:40.59 CSV.dial :waste @13:29:41.17 CS.seek 0ml @13:29:48.89 CSV.dial :flush @13:29:49.47 CS.seek 3ml @13:29:57.31 delay 5 seconds @13:30:02.33 CSV.dial :air @13:30:02.85 CS.seek 5ml @13:30:08.37 CSV.dial :air @13:30:08.55 CS.seek 0ml @13:30:20.74 delay 7 seconds @13:30:27.77 CS.seek 3ml @13:30:35.84 CSV.dial :waste @13:30:36.58 CS.seek 0ml @13:30:44.22 Garaging FlushPuck in Collection Clamp @13:30:44.29 Elbow.reconfigure :tight @13:30:44.33 Elbow.seek CC @13:30:44.60 Elbow.reconfigure :loose @13:30:44.70 Forearm.seek CC @13:30:45.65 Hand.close @13:30:46.33 CC.seek :open @13:30:51.39 Forearm.seek :retract @13:30:52.39 Elbow.reconfigure :tight @13:30:52.44 Elbow.seek :garage @13:30:55.43 Elbow.reconfigure :loose @13:30:55.53 Forearm.reconfigure :tight @13:30:55.58 Forearm.seek :garage @13:30:56.61 Hand.open @13:30:57.26 Forearm.reconfigure :loose @13:30:57.31 Forearm.seek :retract @13:30:58.25 da sampling at most 1000ml, wcr at most 1000ml @13:30:58.29 DAfans.email "Started da in tube 5 at 13:30:58UTC06-Dec-23\n{1 => 33,\n 2 => 33,\n 3 => 31,\n 4 => 21,\n 5 => 12,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@13:26:41, 31% flow, 16.1C, 31% humidity, 15.1psia, 13.695V, 0.296A, 161.242Ah, 4.06W",Subject: "da sampling at most 1000ml, wcr at most 1000ml" @13:30:58.32
Loading sh1 puck from tube 5 into Collection Clamp @13:30:58.58 SC.reconfigure :tight @13:30:58.64 SC.seek 5 @13:31:02.20 SC.configure :limp @13:31:02.46 Elbow.reconfigure :tight @13:31:02.51 Elbow.seek 5 @13:31:02.71 upsync @13:31:04.49
Forearm.reconfigure :tight @13:31:04.54 Forearm.seek 5 + 450 counts @13:31:05.55 SE.reconfigure :pick @13:31:05.61 SE.seek :beamBreak @13:31:08.04 SE.configure :default @13:31:08.21 Elbow.seek 5 @13:31:08.39 Forearm.seek 5 + 300 counts @13:31:08.81 Forearm.reconfigure :loose @13:31:08.86 Hand.close @13:31:09.47 SE.seek :down @13:31:09.67
Forearm.seek :retract @13:31:10.73 Elbow.seek CC @13:31:11.47 SE.stop @13:31:12.34
Elbow.reconfigure :loose @13:31:12.44 Forearm.reconfigure :tight @13:31:12.49 Forearm.seek CC @13:31:13.82 CC.seek :closed @13:31:18.35 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.19 @13:31:18.36 Hand.open @13:31:19.01 Forearm.reconfigure :loose @13:31:19.06 Forearm.seek :retract @13:31:20.12 collectSample 1000ml w/bubble point of 24psi @13:31:20.14 Priming 7ml @13:31:20.15 Intake.select :close @13:31:20.16 Exhaust.select :close @13:31:20.17 SS.hold @13:31:20.38 Calibrating pressure and temperature sensors @13:31:20.41 CTV.dial :air @13:31:20.42 SSV.dial :spit,avoiding: :exhaust @13:31:20.51 SS.reconfigure :fast @13:31:20.56 SS.seek 5ml @13:31:23.37 SSV.dial CBV @13:31:23.39
CBV.dial SSV @13:31:24.72 delay 3.8 seconds @13:31:28.65 Sensor limits: Intake max 101.6psia; Outlet max 94.2psia @13:31:28.66 SSV.dial :spit @13:31:30.00 SS.seek 0ml @13:31:32.69 SSV.dial CBV @13:31:34.00 CTV.dial :intake @13:31:34.79 SSV.dial CBV @13:31:34.81
CBV.dial SSV @13:31:34.96 Allowing 40s for 7.0ml of remaining 7.0ml @13:31:34.98 Intake.select :open @13:31:37.15 Intake @14.5psia, vacuum from 9.0 to 12.0psi @13:31:37.16 SS.reconfigure :inhaling 2.20ml/s @13:31:37.21 SS.seek 7ml @13:31:44.02 Stroke completed -- waiting for pressure to equalize ... @13:31:44.03 SS.reconfigure :exhale @13:31:44.07 SS.hold @13:31:51.64 Intake.select :close @13:31:51.79 SSV.dial :exhaust @13:31:52.53 Exhaust.select :open @13:31:52.65 SS.seek 0ml @13:31:56.67 Exhaust.select :close @13:31:56.78 Sampling 1000.0ml @13:31:56.79 Intake.select :close @13:31:56.81 Exhaust.select :close @13:31:56.82 SS.reconfigure :default @13:31:56.86 SS.hold @13:31:57.07 CTV.dial :intake @13:31:57.25 SSV.dial CBV @13:31:57.28
CBV.dial SSV @13:31:58.04 Allowing 130s for 25.0ml of remaining 1000.0ml @13:31:58.05 Intake.select :open @13:32:00.21 Intake @15.0psia, vacuum from 9.5 to 12.5psi @13:32:00.22 SS.reconfigure :inhaling 2.20ml/s @13:32:00.27 SS.seek 25ml @13:32:08.40 Vacuum too high -- waiting up to 40 seconds @13:32:08.41 SS.reconfigure :exhale @13:32:08.47 SS.hold @13:32:13.72 Intake @15.9psia, vacuum from 10.0 to 13.0psi @13:32:13.73 SS.reconfigure :inhaling 1.76ml/s @13:32:13.78 SS.seek 25ml @13:32:20.81 Vacuum too high -- waiting up to 40 seconds @13:32:20.82 SS.reconfigure :exhale @13:32:20.87 SS.hold @13:32:27.03 Intake @18.3psia, vacuum from 10.0 to 13.0psi @13:32:27.05 SS.reconfigure :inhaling 1.41ml/s @13:32:27.09 SS.seek 25ml @13:32:29.84 Stroke completed -- waiting for pressure to equalize ... @13:32:29.85 SS.reconfigure :exhale @13:32:29.90 SS.hold @13:32:50.72 Intake.select :close @13:32:50.87 SSV.dial :exhaust @13:32:51.63 Exhaust.select :open @13:32:51.74 SS.seek 0ml @13:33:00.56 Exhaust.select :close @13:33:00.67 SSV.dial CBV @13:33:00.70
CBV.dial SSV @13:33:01.46 Allowing 130s for 25.0ml of remaining 975.0ml @13:33:01.47 Intake.select :open @13:33:03.63 Intake @15.8psia, vacuum from 10.0 to 13.0psi @13:33:03.64 SS.reconfigure :inhaling 1.41ml/s @13:33:03.69 SS.seek 25ml @13:33:23.12 Vacuum too high -- waiting up to 40 seconds @13:33:23.13 SS.reconfigure :exhale @13:33:23.18 SS.hold @13:33:31.88 Intake @18.1psia, vacuum from 10.0 to 13.0psi @13:33:31.90 SS.reconfigure :inhaling 1.13ml/s @13:33:31.95 SS.seek 25ml @13:33:34.33 Stroke completed -- waiting for pressure to equalize ... @13:33:34.34 SS.reconfigure :exhale @13:33:34.38 SS.hold @13:33:58.96 Intake.select :close @13:33:59.11 SSV.dial :exhaust @13:33:59.88 Exhaust.select :open @13:33:59.99 SS.seek 0ml @13:34:09.02 Exhaust.select :close @13:34:09.12 SSV.dial CBV @13:34:09.15
CBV.dial SSV @13:34:09.91 Allowing 130s for 25.0ml of remaining 950.0ml @13:34:09.92 Intake.select :open @13:34:12.08 Intake @15.8psia, vacuum from 10.0 to 13.0psi @13:34:12.10 SS.reconfigure :inhaling 1.13ml/s @13:34:12.14 SS.seek 25ml @13:34:36.37 Stroke completed -- waiting for pressure to equalize ... @13:34:36.38 SS.reconfigure :exhale @13:34:36.43 SS.hold @13:35:05.00 Intake.select :close @13:35:05.16 SSV.dial :exhaust @13:35:05.92 Exhaust.select :open @13:35:06.03 SS.seek 0ml @13:35:14.81 Exhaust.select :close @13:35:14.92 SSV.dial CBV @13:35:14.94
CBV.dial SSV @13:35:15.70 Allowing 130s for 25.0ml of remaining 925.0ml @13:35:15.71 Intake.select :open @13:35:17.88 Intake @16.0psia, vacuum from 10.0 to 13.0psi @13:35:17.90 SS.reconfigure :inhaling 1.13ml/s @13:35:17.95 SS.seek 25ml @13:35:42.68 Stroke completed -- waiting for pressure to equalize ... @13:35:42.69 SS.reconfigure :exhale @13:35:42.74 SS.hold @13:36:11.31 Intake.select :close @13:36:11.47 SSV.dial :exhaust @13:36:12.23 Exhaust.select :open @13:36:12.35 SS.seek 0ml @13:36:21.14 Exhaust.select :close @13:36:21.25 SSV.dial CBV @13:36:21.27
CBV.dial SSV @13:36:22.05 Allowing 130s for 25.0ml of remaining 900.0ml @13:36:22.06 Intake.select :open @13:36:24.22 Intake @15.8psia, vacuum from 10.0 to 13.0psi @13:36:24.23 SS.reconfigure :inhaling 1.13ml/s @13:36:24.28 SS.seek 25ml @13:36:49.09 Stroke completed -- waiting for pressure to equalize ... @13:36:49.10 SS.reconfigure :exhale @13:36:49.15 SS.hold @13:37:11.62 Intake.select :close @13:37:11.77 SSV.dial :exhaust @13:37:12.53 Exhaust.select :open @13:37:12.64 SS.seek 0ml @13:37:21.57 Exhaust.select :close @13:37:21.68 SSV.dial CBV @13:37:21.72
CBV.dial SSV @13:37:22.47 Allowing 130s for 25.0ml of remaining 875.0ml @13:37:22.48 Intake.select :open @13:37:24.64 Intake @16.0psia, vacuum from 10.0 to 13.0psi @13:37:24.65 SS.reconfigure :inhaling 1.13ml/s @13:37:24.70 SS.seek 25ml @13:37:49.07 Stroke completed -- waiting for pressure to equalize ... @13:37:49.08 SS.reconfigure :exhale @13:37:49.13 SS.hold @13:38:09.43 Intake.select :close @13:38:09.58 SSV.dial :exhaust @13:38:10.35 Exhaust.select :open @13:38:10.46 SS.seek 0ml @13:38:19.26 Exhaust.select :close @13:38:19.37 SSV.dial CBV @13:38:19.39
CBV.dial SSV @13:38:20.17 Allowing 130s for 25.0ml of remaining 850.0ml @13:38:20.18 Intake.select :open @13:38:22.35 Intake @16.0psia, vacuum from 10.0 to 13.0psi @13:38:22.36 SS.reconfigure :inhaling 1.13ml/s @13:38:22.41 SS.seek 25ml @13:38:46.81 Stroke completed -- waiting for pressure to equalize ... @13:38:46.82 SS.reconfigure :exhale @13:38:46.86 SS.hold @13:39:09.29 Intake.select :close @13:39:09.45 SSV.dial :exhaust @13:39:10.22 Exhaust.select :open @13:39:10.33 SS.seek 0ml @13:39:19.09 Exhaust.select :close @13:39:19.19 SSV.dial CBV @13:39:19.26
CBV.dial SSV @13:39:20.03 Allowing 130s for 25.0ml of remaining 825.0ml @13:39:20.04 Intake.select :open @13:39:22.20 Intake @16.0psia, vacuum from 10.0 to 13.0psi @13:39:22.23 SS.reconfigure :inhaling 1.13ml/s @13:39:22.27 SS.seek 25ml @13:39:47.12 Stroke completed -- waiting for pressure to equalize ... @13:39:47.13 SS.reconfigure :exhale @13:39:47.17 SS.hold @13:40:09.52 Intake.select :close @13:40:09.68 SSV.dial :exhaust @13:40:10.45 Exhaust.select :open @13:40:10.56 SS.seek 0ml @13:40:19.31 Exhaust.select :close @13:40:19.41 SSV.dial CBV @13:40:19.43
CBV.dial SSV @13:40:20.20 Allowing 130s for 25.0ml of remaining 800.0ml @13:40:20.21 Intake.select :open @13:40:22.37 Intake @16.0psia, vacuum from 10.0 to 13.0psi @13:40:22.38 SS.reconfigure :inhaling 1.13ml/s @13:40:22.43 SS.seek 25ml @13:40:46.42 Vacuum too high -- waiting up to 40 seconds @13:40:46.43 SS.reconfigure :exhale @13:40:46.49 SS.hold @13:40:55.14 Intake @16.8psia, vacuum from 10.0 to 13.0psi @13:40:55.16 SS.reconfigure :inhaling 0.91ml/s @13:40:55.20 SS.seek 25ml @13:40:56.52 Stroke completed -- waiting for pressure to equalize ... @13:40:56.53 SS.reconfigure :exhale @13:40:56.58 SS.hold @13:41:07.88 Intake.select :close @13:41:08.03 SSV.dial :exhaust @13:41:08.79 Exhaust.select :open @13:41:08.90 SS.seek 0ml @13:41:17.82 Exhaust.select :close @13:41:17.93 SSV.dial CBV @13:41:17.95
CBV.dial SSV @13:41:18.73 Allowing 130s for 25.0ml of remaining 775.0ml @13:41:18.74 Intake.select :open @13:41:20.91 Intake @16.0psia, vacuum from 10.0 to 13.0psi @13:41:20.92 SS.reconfigure :inhaling 0.91ml/s @13:41:20.97 SS.seek 25ml @13:41:50.50 Stroke completed -- waiting for pressure to equalize ... @13:41:50.52 SS.reconfigure :exhale @13:41:50.56 SS.hold @13:42:06.92 Intake.select :close @13:42:07.08 SSV.dial :exhaust @13:42:07.83 Exhaust.select :open @13:42:07.95 SS.seek 0ml @13:42:16.94 Exhaust.select :close @13:42:17.06 SSV.dial CBV @13:42:17.10
CBV.dial SSV @13:42:17.84 Allowing 130s for 25.0ml of remaining 750.0ml @13:42:17.85 Intake.select :open @13:42:20.01 Intake @16.0psia, vacuum from 10.0 to 13.0psi @13:42:20.02 SS.reconfigure :inhaling 0.91ml/s @13:42:20.07 SS.seek 25ml @13:42:49.38 Stroke completed -- waiting for pressure to equalize ... @13:42:49.39 SS.reconfigure :exhale @13:42:49.44 SS.hold @13:43:05.73 Intake.select :close @13:43:05.88 SSV.dial :exhaust @13:43:06.66 Exhaust.select :open @13:43:06.78 SS.seek 0ml @13:43:15.50 Exhaust.select :close @13:43:15.61 SSV.dial CBV @13:43:15.63
CBV.dial SSV @13:43:16.41 Allowing 130s for 25.0ml of remaining 725.0ml @13:43:16.42 Intake.select :open @13:43:18.59 Intake @16.0psia, vacuum from 10.0 to 13.0psi @13:43:18.60 SS.reconfigure :inhaling 0.91ml/s @13:43:18.65 SS.seek 25ml @13:43:48.49 Stroke completed -- waiting for pressure to equalize ... @13:43:48.50 SS.reconfigure :exhale @13:43:48.54 SS.hold @13:44:04.83 Intake.select :close @13:44:04.98 SSV.dial :exhaust @13:44:05.74 Exhaust.select :open @13:44:05.85 SS.seek 0ml @13:44:14.52 Exhaust.select :close @13:44:14.63 SSV.dial CBV @13:44:14.65
CBV.dial SSV @13:44:15.43 Allowing 130s for 25.0ml of remaining 700.0ml @13:44:15.75 Intake.select :open @13:44:18.03 Intake @15.9psia, vacuum from 10.0 to 13.0psi @13:44:18.05 SS.reconfigure :inhaling 0.91ml/s @13:44:18.09 SS.seek 25ml @13:44:47.80 Stroke completed -- waiting for pressure to equalize ... @13:44:47.81 SS.reconfigure :exhale @13:44:47.86 SS.hold @13:45:06.20 Intake.select :close @13:45:06.35 SSV.dial :exhaust @13:45:07.11 Exhaust.select :open @13:45:07.23 SS.seek 0ml @13:45:15.99 Exhaust.select :close @13:45:16.09 SSV.dial CBV @13:45:16.11
CBV.dial SSV @13:45:16.88 Allowing 130s for 25.0ml of remaining 675.0ml @13:45:16.89 Intake.select :open @13:45:19.06 Intake @16.0psia, vacuum from 10.0 to 13.0psi @13:45:19.07 SS.reconfigure :inhaling 0.91ml/s @13:45:19.12 SS.seek 25ml @13:45:49.08 Stroke completed -- waiting for pressure to equalize ... @13:45:49.09 SS.reconfigure :exhale @13:45:49.13 SS.hold @13:46:07.68 Intake.select :close @13:46:07.84 SSV.dial :exhaust @13:46:08.60 Exhaust.select :open @13:46:08.71 SS.seek 0ml @13:46:17.49 Exhaust.select :close @13:46:17.60 SSV.dial CBV @13:46:17.64
CBV.dial SSV @13:46:18.38 Allowing 130s for 25.0ml of remaining 650.0ml @13:46:18.39 Intake.select :open @13:46:20.56 Intake @15.9psia, vacuum from 10.0 to 13.0psi @13:46:20.57 SS.reconfigure :inhaling 0.91ml/s @13:46:20.62 SS.seek 25ml @13:46:50.30 Stroke completed -- waiting for pressure to equalize ... @13:46:50.31 SS.reconfigure :exhale @13:46:50.35 SS.hold @13:47:10.85 Intake.select :close @13:47:11.00 SSV.dial :exhaust @13:47:11.76 Exhaust.select :open @13:47:11.87 SS.seek 0ml @13:47:20.88 Exhaust.select :close @13:47:20.98 SSV.dial CBV @13:47:21.00
CBV.dial SSV @13:47:21.78 Allowing 130s for 25.0ml of remaining 625.0ml @13:47:21.79 Intake.select :open @13:47:23.96 Intake @15.9psia, vacuum from 10.0 to 13.0psi @13:47:23.97 SS.reconfigure :inhaling 0.91ml/s @13:47:24.02 SS.seek 25ml @13:47:53.31 Stroke completed -- waiting for pressure to equalize ... @13:47:53.32 SS.reconfigure :exhale @13:47:53.37 SS.hold @13:48:13.88 Intake.select :close @13:48:14.04 SSV.dial :exhaust @13:48:14.82 Exhaust.select :open @13:48:14.94 SS.seek 0ml @13:48:23.66 Exhaust.select :close @13:48:23.76 SSV.dial CBV @13:48:23.79
CBV.dial SSV @13:48:24.56 Allowing 130s for 25.0ml of remaining 600.0ml @13:48:24.57 Intake.select :open @13:48:26.74 Intake @16.0psia, vacuum from 10.0 to 13.0psi @13:48:26.76 SS.reconfigure :inhaling 0.91ml/s @13:48:26.81 SS.seek 25ml @13:48:56.62 Stroke completed -- waiting for pressure to equalize ... @13:48:56.63 SS.reconfigure :exhale @13:48:56.68 SS.hold @13:49:17.16 Intake.select :close @13:49:17.32 SSV.dial :exhaust @13:49:18.08 Exhaust.select :open @13:49:18.20 SS.seek 0ml @13:49:27.01 Exhaust.select :close @13:49:27.12 SSV.dial CBV @13:49:27.14
CBV.dial SSV @13:49:27.92 Allowing 130s for 25.0ml of remaining 575.0ml @13:49:27.93 Intake.select :open @13:49:30.10 Intake @16.0psia, vacuum from 10.0 to 13.0psi @13:49:30.11 SS.reconfigure :inhaling 0.91ml/s @13:49:30.16 SS.seek 25ml @13:49:59.94 Vacuum too high -- waiting up to 40 seconds @13:49:59.96 SS.reconfigure :exhale @13:50:00.01 SS.hold @13:50:11.26 Intake @16.8psia, vacuum from 10.0 to 13.0psi @13:50:11.28 SS.reconfigure :inhaling 0.73ml/s @13:50:11.32 SS.seek 25ml @13:50:11.81 Stroke completed -- waiting for pressure to equalize ... @13:50:11.82 SS.reconfigure :exhale @13:50:11.87 SS.hold @13:50:21.67 Intake.select :close @13:50:21.83 SSV.dial :exhaust @13:50:22.59 Exhaust.select :open @13:50:22.71 SS.seek 0ml @13:50:31.45 Exhaust.select :close @13:50:31.56 SSV.dial CBV @13:50:31.58
CBV.dial SSV @13:50:32.36 Allowing 130s for 25.0ml of remaining 550.0ml @13:50:32.37 Intake.select :open @13:50:34.53 Intake @16.1psia, vacuum from 10.0 to 13.0psi @13:50:34.55 SS.reconfigure :inhaling 0.73ml/s @13:50:34.60 SS.seek 25ml @13:51:10.79 Stroke completed -- waiting for pressure to equalize ... @13:51:10.80 SS.reconfigure :exhale @13:51:10.85 SS.hold @13:51:29.60 Intake.select :close @13:51:29.76 SSV.dial :exhaust @13:51:30.51 Exhaust.select :open @13:51:30.63 SS.seek 0ml @13:51:39.65 Exhaust.select :close @13:51:39.76 SSV.dial CBV @13:51:39.79
CBV.dial SSV @13:51:40.54 Allowing 130s for 25.0ml of remaining 525.0ml @13:51:40.56 Intake.select :open @13:51:42.73 Intake @16.0psia, vacuum from 10.0 to 13.0psi @13:51:42.74 SS.reconfigure :inhaling 0.73ml/s @13:51:42.79 SS.seek 25ml @13:52:18.95 Stroke completed -- waiting for pressure to equalize ... @13:52:18.96 SS.reconfigure :exhale @13:52:19.01 SS.hold @13:52:37.49 Intake.select :close @13:52:37.64 SSV.dial :exhaust @13:52:38.40 Exhaust.select :open @13:52:38.52 SS.seek 0ml @13:52:47.28 Exhaust.select :close @13:52:47.39 SSV.dial CBV @13:52:47.43
CBV.dial SSV @13:52:48.17 Allowing 130s for 25.0ml of remaining 500.0ml @13:52:48.18 Intake.select :open @13:52:50.35 Intake @16.0psia, vacuum from 10.0 to 13.0psi @13:52:50.36 SS.reconfigure :inhaling 0.73ml/s @13:52:50.41 SS.seek 25ml @13:53:26.82 Stroke completed -- waiting for pressure to equalize ... @13:53:26.83 SS.reconfigure :exhale @13:53:26.88 SS.hold @13:53:47.36 Intake.select :close @13:53:47.51 SSV.dial :exhaust @13:53:48.28 Exhaust.select :open @13:53:48.40 SS.seek 0ml @13:53:57.37 Exhaust.select :close @13:53:57.47 SSV.dial CBV @13:53:57.49
CBV.dial SSV @13:53:58.26 Allowing 130s for 25.0ml of remaining 475.0ml @13:53:58.27 Intake.select :open @13:54:00.44 Intake @16.0psia, vacuum from 10.0 to 13.0psi @13:54:00.45 SS.reconfigure :inhaling 0.73ml/s @13:54:00.50 SS.seek 25ml @13:54:36.40 Stroke completed -- waiting for pressure to equalize ... @13:54:36.41 SS.reconfigure :exhale @13:54:36.46 SS.hold @13:54:58.81 Intake.select :close @13:54:58.96 SSV.dial :exhaust @13:54:59.73 Exhaust.select :open @13:54:59.84 SS.seek 0ml @13:55:08.82 Exhaust.select :close @13:55:08.93 SSV.dial CBV @13:55:08.95
CBV.dial SSV @13:55:09.71 Allowing 130s for 25.0ml of remaining 450.0ml @13:55:09.72 Intake.select :open @13:55:11.89 Intake @16.0psia, vacuum from 10.0 to 13.0psi @13:55:11.90 SS.reconfigure :inhaling 0.73ml/s @13:55:11.95 SS.seek 25ml @13:55:47.97 Stroke completed -- waiting for pressure to equalize ... @13:55:47.98 SS.reconfigure :exhale @13:55:48.03 SS.hold @13:56:11.47 Intake.select :close @13:56:11.62 SSV.dial :exhaust @13:56:12.38 Exhaust.select :open @13:56:12.50 SS.seek 0ml @13:56:21.19 Exhaust.select :close @13:56:21.31 SSV.dial CBV @13:56:21.35
CBV.dial SSV @13:56:22.09 Allowing 130s for 25.0ml of remaining 425.0ml @13:56:22.10 Intake.select :open @13:56:24.26 Intake @16.0psia, vacuum from 10.0 to 13.0psi @13:56:24.27 SS.reconfigure :inhaling 0.73ml/s @13:56:24.32 SS.seek 25ml @13:56:58.65 Vacuum too high -- waiting up to 40 seconds @13:56:58.67 SS.reconfigure :exhale @13:56:58.72 SS.hold @13:57:10.02 Intake @16.1psia, vacuum from 10.0 to 13.0psi @13:57:10.03 SS.reconfigure :inhaling 0.59ml/s @13:57:10.08 SS.seek 25ml @13:57:12.60 Stroke completed -- waiting for pressure to equalize ... @13:57:12.61 SS.reconfigure :exhale @13:57:12.65 SS.hold @13:57:25.51 Intake.select :close @13:57:25.67 SSV.dial :exhaust @13:57:26.43 Exhaust.select :open @13:57:26.54 SS.seek 0ml @13:57:35.25 Exhaust.select :close @13:57:35.36 SSV.dial CBV @13:57:35.39
CBV.dial SSV @13:57:36.15 Allowing 130s for 25.0ml of remaining 400.0ml @13:57:36.16 Intake.select :open @13:57:38.32 Intake @16.1psia, vacuum from 10.0 to 13.0psi @13:57:38.34 SS.reconfigure :inhaling 0.59ml/s @13:57:38.39 SS.seek 25ml @13:58:23.10 Stroke completed -- waiting for pressure to equalize ... @13:58:23.11 SS.reconfigure :exhale @13:58:23.15 SS.hold @13:58:44.11 Intake.select :close @13:58:44.26 SSV.dial :exhaust @13:58:45.03 Exhaust.select :open @13:58:45.14 SS.seek 0ml @13:58:54.11 Exhaust.select :close @13:58:54.22 SSV.dial CBV @13:58:54.24
CBV.dial SSV @13:58:55.00 Allowing 130s for 25.0ml of remaining 375.0ml @13:58:55.02 Intake.select :open @13:58:57.18 Intake @16.1psia, vacuum from 10.0 to 13.0psi @13:58:57.20 SS.reconfigure :inhaling 0.59ml/s @13:58:57.25 SS.seek 25ml @13:59:41.77 Stroke completed -- waiting for pressure to equalize ... @13:59:41.78 SS.reconfigure :exhale @13:59:41.82 SS.hold @14:00:04.25 Intake.select :close @14:00:04.41 SSV.dial :exhaust @14:00:05.17 Exhaust.select :open @14:00:05.29 SS.seek 0ml @14:00:14.05 Exhaust.select :close @14:00:14.16 SSV.dial CBV @14:00:14.18
CBV.dial SSV @14:00:14.96 Allowing 130s for 25.0ml of remaining 350.0ml @14:00:14.98 Intake.select :open @14:00:17.14 Intake @16.1psia, vacuum from 10.0 to 13.0psi @14:00:17.15 SS.reconfigure :inhaling 0.59ml/s @14:00:17.20 SS.seek 25ml @14:01:01.81 Stroke completed -- waiting for pressure to equalize ... @14:01:01.82 SS.reconfigure :exhale @14:01:01.87 SS.hold @14:01:26.34 Intake.select :close @14:01:26.49 SSV.dial :exhaust @14:01:27.25 Exhaust.select :open @14:01:27.37 SS.seek 0ml @14:01:36.14 Exhaust.select :close @14:01:36.25 SSV.dial CBV @14:01:36.27
CBV.dial SSV @14:01:37.05 Allowing 130s for 25.0ml of remaining 325.0ml @14:01:37.06 Intake.select :open @14:01:39.22 Intake @16.1psia, vacuum from 10.0 to 13.0psi @14:01:39.24 SS.reconfigure :inhaling 0.59ml/s @14:01:39.29 SS.seek 25ml @14:02:12.58 Vacuum too high -- waiting up to 40 seconds @14:02:12.60 SS.reconfigure :exhale @14:02:12.65 SS.hold @14:02:22.60 Intake @17.5psia, vacuum from 10.0 to 13.0psi @14:02:22.61 SS.reconfigure :inhaling 0.47ml/s @14:02:22.66 SS.seek 25ml @14:02:37.15 Stroke completed -- waiting for pressure to equalize ... @14:02:37.16 SS.reconfigure :exhale @14:02:37.21 SS.hold @14:02:53.50 Intake.select :close @14:02:53.65 SSV.dial :exhaust @14:02:54.40 Exhaust.select :open @14:02:54.52 SS.seek 0ml @14:03:03.53 Exhaust.select :close @14:03:03.64 SSV.dial CBV @14:03:03.66
CBV.dial SSV @14:03:04.42 Allowing 130s for 25.0ml of remaining 300.0ml @14:03:04.43 Intake.select :open @14:03:06.60 Intake @15.9psia, vacuum from 10.0 to 13.0psi @14:03:06.61 SS.reconfigure :inhaling 0.47ml/s @14:03:06.66 SS.seek 25ml @14:04:02.18 Stroke completed -- waiting for pressure to equalize ... @14:04:02.21 SS.reconfigure :exhale @14:04:02.26 SS.hold @14:04:24.69 Intake.select :close @14:04:24.84 SSV.dial :exhaust @14:04:25.61 Exhaust.select :open @14:04:25.72 SS.seek 0ml @14:04:34.50 Exhaust.select :close @14:04:34.61 SSV.dial CBV @14:04:34.64
CBV.dial SSV @14:04:35.39 Allowing 130s for 25.0ml of remaining 275.0ml @14:04:35.40 Intake.select :open @14:04:37.57 Intake @16.2psia, vacuum from 10.0 to 13.0psi @14:04:37.58 SS.reconfigure :inhaling 0.47ml/s @14:04:37.63 SS.seek 25ml @14:05:33.18 Stroke completed -- waiting for pressure to equalize ... @14:05:33.19 SS.reconfigure :exhale @14:05:33.24 SS.hold @14:05:55.95 Intake.select :close @14:05:56.10 SSV.dial :exhaust @14:05:56.85 Exhaust.select :open @14:05:56.97 SS.seek 0ml @14:06:05.74 Exhaust.select :close @14:06:05.85 SSV.dial CBV @14:06:05.88
CBV.dial SSV @14:06:06.65 Allowing 130s for 25.0ml of remaining 250.0ml @14:06:06.66 Intake.select :open @14:06:08.83 Intake @16.1psia, vacuum from 10.0 to 13.0psi @14:06:08.85 SS.reconfigure :inhaling 0.47ml/s @14:06:08.90 SS.seek 25ml @14:07:04.51 Stroke completed -- waiting for pressure to equalize ... @14:07:04.52 SS.reconfigure :exhale @14:07:04.56 SS.hold @14:07:31.51 Intake.select :close @14:07:31.66 SSV.dial :exhaust @14:07:32.42 Exhaust.select :open @14:07:32.54 SS.seek 0ml @14:07:41.34 Exhaust.select :close @14:07:41.45 SSV.dial CBV @14:07:41.48
CBV.dial SSV @14:07:42.28 Allowing 130s for 25.0ml of remaining 225.0ml @14:07:42.29 Intake.select :open @14:07:44.46 Intake @16.2psia, vacuum from 10.0 to 13.0psi @14:07:44.47 SS.reconfigure :inhaling 0.47ml/s @14:07:44.52 SS.seek 25ml @14:08:39.90 Stroke completed -- waiting for pressure to equalize ... @14:08:39.91 SS.reconfigure :exhale @14:08:39.95 SS.hold @14:09:08.78 Intake.select :close @14:09:08.93 SSV.dial :exhaust @14:09:09.69 Exhaust.select :open @14:09:09.81 SS.seek 0ml @14:09:18.79 Exhaust.select :close @14:09:18.89 SSV.dial CBV @14:09:18.91
CBV.dial SSV @14:09:19.66 Allowing 130s for 25.0ml of remaining 200.0ml @14:09:19.68 Intake.select :open @14:09:21.84 Intake @16.3psia, vacuum from 10.0 to 13.0psi @14:09:21.85 SS.reconfigure :inhaling 0.47ml/s @14:09:21.90 SS.seek 25ml @14:10:17.29 Stroke completed -- waiting for pressure to equalize ... @14:10:17.30 SS.reconfigure :exhale @14:10:17.34 SS.hold @14:10:50.29 Intake.select :close @14:10:50.44 SSV.dial :exhaust @14:10:51.22 Exhaust.select :open @14:10:51.33 SS.seek 0ml @14:11:00.33 Exhaust.select :close @14:11:00.44 SSV.dial CBV @14:11:00.47
CBV.dial SSV @14:11:01.24 Allowing 130s for 25.0ml of remaining 175.0ml @14:11:01.25 Intake.select :open @14:11:03.42 Intake @16.2psia, vacuum from 10.0 to 13.0psi @14:11:03.43 SS.reconfigure :inhaling 0.47ml/s @14:11:03.91 SS.seek 25ml @14:11:59.00 Stroke completed -- waiting for pressure to equalize ... @14:11:59.01 SS.reconfigure :exhale @14:11:59.06 SS.hold @14:12:31.74 Intake.select :close @14:12:31.89 SSV.dial :exhaust @14:12:32.65 Exhaust.select :open @14:12:32.77 SS.seek 0ml @14:12:41.98 Exhaust.select :close @14:12:42.16 SSV.dial CBV @14:12:42.19
CBV.dial SSV @14:12:42.96 Allowing 130s for 25.0ml of remaining 150.0ml @14:12:42.97 Intake.select :open @14:12:45.14 Intake @16.4psia, vacuum from 10.0 to 13.0psi @14:12:45.15 SS.reconfigure :inhaling 0.47ml/s @14:12:45.20 SS.seek 25ml @14:13:24.63 Vacuum too high -- waiting up to 40 seconds @14:13:24.65 SS.reconfigure :exhale @14:13:24.70 SS.hold @14:13:39.22 Intake @18.2psia, vacuum from 10.0 to 13.0psi @14:13:39.23 SS.reconfigure :inhaling 0.38ml/s @14:13:39.28 SS.seek 25ml @14:13:52.98 Vacuum too high -- waiting up to 40 seconds @14:13:52.99 SS.reconfigure :exhale @14:13:53.04 SS.hold @14:13:58.87 Intake @17.9psia, vacuum from 10.0 to 13.0psi @14:13:58.89 SS.reconfigure :inhaling 0.31ml/s @14:13:58.94 SS.seek 25ml @14:14:07.01 Stroke completed -- waiting for pressure to equalize ... @14:14:07.03 SS.reconfigure :exhale @14:14:07.07 SS.hold @14:14:25.49 Intake.select :close @14:14:25.64 SSV.dial :exhaust @14:14:26.39 Exhaust.select :open @14:14:26.51 SS.seek 0ml @14:14:35.74 Exhaust.select :close @14:14:35.86 SSV.dial CBV @14:14:35.88
CBV.dial SSV @14:14:36.64 Allowing 130s for 25.0ml of remaining 125.0ml @14:14:36.65 Intake.select :open @14:14:38.82 Intake @16.2psia, vacuum from 10.0 to 13.0psi @14:14:38.83 SS.reconfigure :inhaling 0.31ml/s @14:14:38.88 SS.seek 25ml @14:16:05.45 Stroke completed -- waiting for pressure to equalize ... @14:16:05.46 SS.reconfigure :exhale @14:16:05.51 SS.hold @14:16:30.01 Intake.select :close @14:16:30.17 SSV.dial :exhaust @14:16:30.94 Exhaust.select :open @14:16:31.05 SS.seek 0ml @14:16:40.01 Exhaust.select :close @14:16:40.49 SSV.dial CBV @14:16:40.51
CBV.dial SSV @14:16:41.28 Allowing 130s for 25.0ml of remaining 100.0ml @14:16:41.29 Intake.select :open @14:16:43.46 Intake @16.2psia, vacuum from 10.0 to 13.0psi @14:16:43.48 SS.reconfigure :inhaling 0.31ml/s @14:16:43.52 SS.seek 25ml @14:18:10.17 Stroke completed -- waiting for pressure to equalize ... @14:18:10.18 SS.reconfigure :exhale @14:18:10.23 SS.hold @14:18:38.80 Intake.select :close @14:18:38.95 SSV.dial :exhaust @14:18:39.70 Exhaust.select :open @14:18:40.24 SS.seek 0ml @14:18:49.29 Exhaust.select :close @14:18:49.40 SSV.dial CBV @14:18:49.43
CBV.dial SSV @14:18:50.20 Allowing 130s for 25.0ml of remaining 75.0ml @14:18:50.21 Intake.select :open @14:18:52.39 Intake @16.3psia, vacuum from 10.0 to 13.0psi @14:18:52.40 SS.reconfigure :inhaling 0.31ml/s @14:18:52.45 SS.seek 25ml @14:20:18.99 Stroke completed -- waiting for pressure to equalize ... @14:20:19.00 SS.reconfigure :exhale @14:20:19.05 SS.hold @14:20:47.68 Intake.select :close @14:20:47.83 SSV.dial :exhaust @14:20:49.01 Exhaust.select :open @14:20:49.13 SS.seek 0ml @14:20:57.93 Exhaust.select :close @14:20:58.04 SSV.dial CBV @14:20:58.06
CBV.dial SSV @14:20:58.84 Allowing 130s for 25.0ml of remaining 50.0ml @14:20:58.85 Intake.select :open @14:21:01.02 Intake @16.3psia, vacuum from 10.0 to 13.0psi @14:21:01.03 SS.reconfigure :inhaling 0.31ml/s @14:21:01.08 SS.seek 25ml @14:22:28.68 Stroke completed -- waiting for pressure to equalize ... @14:22:28.69 SS.reconfigure :exhale @14:22:28.74 SS.hold @14:22:59.43 Intake.select :close @14:22:59.59 SSV.dial :exhaust @14:23:00.35 Exhaust.select :open @14:23:00.47 SS.seek 0ml @14:23:09.30 Exhaust.select :close @14:23:09.41 SSV.dial CBV @14:23:09.45
CBV.dial SSV @14:23:10.20 Allowing 130s for 25.0ml of remaining 25.0ml @14:23:10.21 Intake.select :open @14:23:12.37 Intake @16.5psia, vacuum from 10.0 to 13.0psi @14:23:12.38 SS.reconfigure :inhaling 0.31ml/s @14:23:12.43 SS.seek 24.98ml @14:24:38.97 Stroke completed -- waiting for pressure to equalize ... @14:24:38.98 SS.reconfigure :exhale @14:24:39.03 SS.hold @14:25:11.70 Intake.select :close @14:25:11.86 SSV.dial :exhaust @14:25:12.62 Exhaust.select :open @14:25:12.74 SS.seek 0ml @14:25:21.57 Exhaust.select :close @14:25:21.67 Sampled 1000.0ml @14:25:21.69 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) Intake.select :close @14:25:21.76 CSV.dial :waste @14:25:21.91 SSV.dial CBV,avoiding: :exhaust @14:25:21.93
CBV.dial SSV @14:25:22.02 CS.seek 0ml @14:25:22.10 CSV.dial :air @14:25:22.84 CS.seek 10ml @14:25:23.86
CTV.dial :air @14:25:24.63 delay 15 seconds @14:25:39.65 SSV.dial :spit,avoiding: :exhaust @14:25:40.98 CTV.dial CSV,avoiding: :intake @14:25:41.01
SS.seek 0ml @14:25:41.13 SSV.dial CBV,avoiding: :exhaust @14:25:47.23 SSV.dial between CBV and plug @14:25:47.71 CBV.dial between SSV and waste2 @14:25:48.10 CSV.dial between air and CTV @14:25:48.40 CS.seek 8.239ml @14:25:53.29 CTV.dial CSV,avoiding: :air @14:25:53.31
CSV.dial CTV @14:25:53.67 CS.reconfigure :pressurize @14:25:53.72 CS.seek 0ml @14:26:08.70 BubblePoint: Intake @24.2psia, Outlet @1.0psia @14:26:08.71 CBV.dial SSV @14:26:09.06 SS.reconfigure :default @14:26:09.11 SS.seek 10ml @14:26:14.75 delay 15 seconds @14:26:29.88 Evacuated: Intake @23.6psia, Outlet @23.2psia @14:26:29.89 SSV.dial :spit,avoiding: :exhaust @14:26:30.95 SS.reconfigure :exhale @14:26:30.99 SS.seek 0ml @14:26:35.92 SS.reconfigure :fast @14:26:35.97 SS.seek 5ml @14:26:38.82 SSV.dial CBV,avoiding: :exhaust @14:26:38.84
CBV.dial SSV @14:26:40.27 CTV.dial :air,avoiding: :intake @14:26:41.62 CSV.dial CTV @14:26:41.65
SS.seek 10ml @14:26:42.86 CSV.dial :air @14:26:43.32 CS.reconfigure :fast @14:26:43.37 CS.seek 0ml @14:26:44.24
SSV.dial :spit,avoiding: :exhaust @14:26:45.56 SS.reconfigure :exhale @14:26:45.61 SS.seek 0ml @14:26:50.28 CS.stop @14:26:50.91
SS.reconfigure :fast @14:26:50.95 SS.seek 5ml @14:26:53.64 SSV.dial CBV,avoiding: :exhaust @14:26:53.65
CBV.dial SSV @14:26:56.00 SSV.dial :spit,avoiding: :exhaust @14:26:57.30 SS.reconfigure :default @14:26:57.35 SS.seek 0ml @14:27:00.61 SS.stop @14:27:00.65 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @14:27:00.66 Intake.select :close @14:27:00.73 CSV.dial :waste @14:27:00.86 SSV.dial CBV,avoiding: :exhaust @14:27:00.88
CBV.dial SSV @14:27:01.52 CS.seek 0ml @14:27:01.60 CSV.dial :air @14:27:02.22
CTV.dial :air @14:27:02.30 delay 15 seconds @14:27:02.35 CS.seek 10ml @14:27:17.33
SSV.dial :spit,avoiding: :exhaust @14:27:18.66 CTV.dial CSV,avoiding: :intake @14:27:18.70
SS.reconfigure :exhale @14:27:18.79 SS.seek 0ml @14:27:19.19 SSV.dial CBV,avoiding: :exhaust @14:27:26.60 SSV.dial between CBV and plug @14:27:27.09 CBV.dial between SSV and waste2 @14:27:27.48 CSV.dial between air and CTV @14:27:27.81 CS.seek 7.47ml @14:27:34.36 CTV.dial CSV,avoiding: :air @14:27:34.38
CSV.dial CTV @14:27:34.71 CS.reconfigure :pressurize @14:27:34.76 CS.seek 0ml @14:27:47.76 BubblePoint: Intake @24.2psia, Outlet @3.3psia @14:27:48.13 CBV.dial SSV @14:27:48.53 SS.reconfigure :default @14:27:48.57 SS.seek 10ml @14:27:54.17 delay 15 seconds @14:28:09.30 Evacuated: Intake @24.0psia, Outlet @23.6psia @14:28:09.32 SSV.dial :spit,avoiding: :exhaust @14:28:10.35 SS.reconfigure :exhale @14:28:10.40 SS.seek 0ml @14:28:15.56 SS.reconfigure :fast @14:28:15.61 SS.seek 5ml @14:28:18.31 SSV.dial CBV,avoiding: :exhaust @14:28:18.33
CBV.dial SSV @14:28:19.68 CTV.dial :air,avoiding: :intake @14:28:21.02 CSV.dial CTV @14:28:21.06
SS.seek 10ml @14:28:22.26 CSV.dial :air @14:28:22.71 CS.reconfigure :fast @14:28:22.76 CS.seek 0ml @14:28:23.57
SSV.dial :spit,avoiding: :exhaust @14:28:24.90 SS.reconfigure :exhale @14:28:24.95 SS.seek 0ml @14:28:29.47 CS.stop @14:28:29.90
SS.reconfigure :fast @14:28:29.95 SS.seek 5ml @14:28:32.91 SSV.dial CBV,avoiding: :exhaust @14:28:32.93
CBV.dial SSV @14:28:35.26 SSV.dial :spit,avoiding: :exhaust @14:28:36.57 SS.reconfigure :default @14:28:36.62 SS.seek 0ml @14:28:39.70 SS.stop @14:28:39.74 Lysing: Adding 2.0ml AqMeOH @14:28:39.76 CBV.dial between CSV and waste1,avoiding: :DBV @14:28:41.25 CTV.dial CSV @14:28:42.57 CSV.dial CSR @14:28:43.35 Pulling 1ml of CSR.AqMeOH @14:28:43.36 CSR.select :AqMeOH @14:28:43.48 CS.reconfigure :default @14:28:43.52 CS.seek 1ml @14:28:47.13 delay 5 seconds @14:28:52.15 CSR.select :air @14:28:52.31 CS.seek 6ml @14:29:05.91 delay 2 seconds @14:29:07.92 CSV.dial :waste2 @14:29:08.37 CS.reconfigure :fast @14:29:08.41 CS.seek 0ml @14:29:22.72 CSV.dial CSR @14:29:23.17 Pulling 2ml of CSR.AqMeOH @14:29:23.18 CSR.select :AqMeOH @14:29:23.30 CS.reconfigure :default @14:29:23.34 CS.seek 2ml @14:29:29.25 delay 5 seconds @14:29:34.27 CSR.select :air @14:29:34.50 CS.seek 5ml @14:29:43.00 delay 2 seconds @14:29:45.02 CSV.dial CTV @14:29:45.48 CS.seek 1ml @14:29:56.67 Before lysing Collection Puck Heater at 12.7C/31.1psia @14:29:56.69 Heating Puck.from 5, :sh1 in Collection Clamp to 70C for 10:00 @14:29:56.71 CH.seek 70C,8:00 @14:32:09.89 CH.hold 10:00,70C @14:32:10.08 Begun lysing Collection Puck Heater at 68C/37.8psia @14:42:10.09 Ended lysing Collection Puck Heater at 70C/36.8psia @14:42:10.11 CS.seek 0ml @14:42:13.47 CTV.dial between spare and air,avoiding: :intake @14:42:14.49 CSV.dial :air @14:42:14.93 CS.seek 5ml @14:42:28.52 CSV.dial CTV @14:42:28.98 delay 2 seconds @14:42:30.99 CSV.dial :air @14:42:31.44 delay 2 seconds @14:42:33.46 CSV.dial CTV @14:42:33.91 delay 2 seconds @14:42:35.93 CSV.dial :air @14:42:36.38 delay 2 seconds @14:42:38.40 CS.seek 0ml @14:42:52.04 Puck @82.2psia after final lysis pressurization @14:42:52.06 Recovering Lysate into Collection syringe @14:42:52.07 CSV.dial CBV @14:42:53.08 CBV.dial CSV @14:42:53.47 CS.seek 5.001ml @14:43:07.27 delay 5 seconds @14:43:12.29 CTV.dial :air @14:43:12.81 CS.seek 7ml @14:43:18.71 delay 5 seconds @14:43:23.73 CSV.dial :air2 @14:43:24.17 CS.seek 10ml @14:43:32.62 CSV.dial between air and CTV,avoiding: CBV @14:43:33.77 Lysing Complete @14:43:33.84 Storing sh1 puck in Collection Clamp to tube 4 @14:43:34.46 SC.reconfigure :tight @14:43:34.51 SC.seek 4 @14:43:38.08 SC.configure :limp @14:43:38.30 Elbow.reconfigure :tight @14:43:38.35 Elbow.seek 4 @14:43:40.06 Forearm.reconfigure :tight @14:43:40.11 Forearm.seek 4 + 450 counts @14:43:41.19 SE.reconfigure :pick @14:43:41.25 SE.seek :beamBreak @14:43:43.12 SE.configure :default @14:43:43.28 Elbow.reconfigure :limp @14:43:43.33 Forearm.reconfigure :loose @14:43:43.37 Forearm.seek 4 @14:43:43.61 Elbow.hold @14:43:43.68 Elbow.reconfigure :tight @14:43:43.78 SE.seek 20.12 Pucks below Top Plate @14:43:44.34 Forearm.force -20 @14:43:44.38 Forearm.seek 4 + 305 counts @14:43:44.79 Forearm.reconfigure :tight @14:43:44.84 Forearm.seek 4 + 450 counts @14:43:45.04 SE.seek 21.12 Pucks below Top Plate @14:43:45.59 Forearm.reconfigure :loose @14:43:45.64 Forearm.seek :retract @14:43:46.59 Elbow.seek CC @14:43:48.26 Elbow.reconfigure :loose @14:43:48.36 Forearm.seek CC @14:43:49.36 Hand.close @14:43:50.02 CC.seek :open @14:43:54.75 Forearm.seek :retract @14:43:55.77 Elbow.reconfigure :tight @14:43:55.82 Elbow.seek 4 @14:43:57.58 Elbow.reconfigure :limp @14:43:57.63 Forearm.seek 4 @14:43:58.62 Elbow.hold @14:43:58.68 Elbow.reconfigure :tight @14:43:58.73 Hand.open @14:43:59.52 Forearm.force -20 @14:43:59.57 Forearm.seek 4 + 317 counts @14:43:59.94 Forearm.reconfigure :tight @14:43:59.99 Forearm.seek 4 + 450 counts @14:44:00.31 SE.seek :down @14:44:01.43
Forearm.reconfigure :loose @14:44:01.48 Forearm.seek :retract @14:44:01.59 SE.stop @14:44:02.44
Tranferring DA @14:44:02.46 Beginning samplePassC @14:44:02.47 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:44:02.66 Elbow.seek FlushPuck::Garage @14:44:04.53 Elbow.reconfigure :loose @14:44:04.64 Forearm.seek :garage @14:44:05.61 Hand.close @14:44:06.39 Forearm.seek :retract @14:44:07.41 Elbow.reconfigure :tight @14:44:07.46 Elbow.seek CC @14:44:10.37 Elbow.reconfigure :loose @14:44:10.47 Forearm.reconfigure :tight @14:44:10.52 Forearm.seek CC @14:44:12.18 CC.seek :closed @14:44:16.74 Collection Clamp onSpring/offSpring for 14/27 samples, on/off torque 2.40 @14:44:16.81 Hand.open @14:44:17.45 Forearm.reconfigure :loose @14:44:17.50 Forearm.seek :retract @14:44:18.46 PSV.dial PSR @14:44:19.85 Pulling 1.5ml of PSR.flush PSR.select :flush @14:44:19.98 PS.seek 1.5ml @14:44:36.09 delay 5 seconds @14:44:41.11 PSV.dial :waste1 @14:44:41.58 PS.seek 0ml @14:44:57.68 PSV.dial PSR @14:44:58.15 PSR.select :air @14:44:58.27 PS.seek 2.5ml @14:45:24.69 PRV.dial CBV,avoiding: :ab2 @14:45:24.70
CBV.dial PRV @14:45:26.46 PSV.dial :mixloop @14:45:27.51 CTV.dial :air,avoiding: :intake @14:45:27.69 PTV.dial :PRVmixing @14:45:29.04 PS.seek 0ml @14:45:55.58 PSV.dial :air @14:45:56.06 PS.seek 2.5ml @14:46:22.43 PSV.dial :mixloop @14:46:22.90 PS.seek 0ml @14:46:49.27 PSV.dial :air @14:46:49.75 PS.seek 2.5ml @14:47:16.11 PSV.dial :mixloop @14:47:16.59 PS.seek 0ml @14:47:42.93 Garaging FlushPuck in Collection Clamp @14:47:43.00 Elbow.reconfigure :tight @14:47:43.05 Elbow.seek CC @14:47:43.88 Elbow.reconfigure :loose @14:47:43.99 Forearm.seek CC @14:47:45.03 Hand.close @14:47:45.65 CC.seek :open @14:47:50.30 Forearm.seek :retract @14:47:51.30 Elbow.reconfigure :tight @14:47:51.35 Elbow.seek :garage @14:47:54.28 Elbow.reconfigure :loose @14:47:54.38 Forearm.reconfigure :tight @14:47:54.43 Forearm.seek :garage @14:47:55.52 Hand.open @14:47:56.20 Forearm.reconfigure :loose @14:47:56.25 Forearm.seek :retract @14:47:57.25 PSV.dial :mixloop @14:47:57.33 PTV.dial :PRVmixing @14:47:57.41 PRV.dial CBV @14:47:57.44
CBV.dial PRV @14:47:57.52 CTV.dial :air,avoiding: :intake @14:47:57.54 Loading lyfil puck from tube 5 into Collection Clamp @14:47:57.78 SC.reconfigure :tight @14:47:57.83 SC.seek 5 @14:48:01.37 SC.configure :limp @14:48:01.59 Elbow.reconfigure :tight @14:48:01.64 Elbow.seek 5 @14:48:03.62 Forearm.reconfigure :tight @14:48:03.67 Forearm.seek 5 + 450 counts @14:48:04.69 SE.reconfigure :pick @14:48:04.75 SE.seek :beamBreak @14:48:07.17 SE.configure :default @14:48:07.33 Elbow.seek 5 @14:48:07.95 Forearm.seek 5 + 300 counts @14:48:08.16 Forearm.reconfigure :loose @14:48:08.20 Hand.close @14:48:08.78 SE.seek :down @14:48:08.99
Forearm.seek :retract @14:48:09.94 Elbow.seek CC @14:48:10.87 SE.stop @14:48:11.56
Elbow.reconfigure :loose @14:48:11.66 Forearm.reconfigure :tight @14:48:11.71 Forearm.seek CC @14:48:13.03 CC.seek :closed @14:48:17.56 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.22 @14:48:17.57 Hand.open @14:48:18.20 Forearm.reconfigure :loose @14:48:18.25 Forearm.seek :retract @14:48:19.21 CTV.dial CSV,avoiding: :intake @14:48:20.55 CSV.dial CTV @14:48:20.88 PS.seek 2.5ml @14:48:47.30 CS.seek 4.5ml @14:49:02.20 delay 8 seconds @14:49:10.22 PSV.dial :air @14:49:10.69 delay 2 seconds @14:49:12.71 PSV.dial :waste2 @14:49:13.45 PS.seek 0ml @14:49:39.77 CBV.dial between PRV and waste1 @14:49:40.09 CSV.dial :air @14:49:41.02 CS.seek 10ml @14:49:55.98 CSV.dial CTV @14:49:56.44 CS.seek 0ml @14:50:22.79 CBV.dial :waste1 @14:50:23.11 delay 5 seconds @14:50:28.13 CTV.dial :air,avoiding: :intake @14:50:29.54 Storing lyfil puck in Collection Clamp to tube 4 @14:50:29.72 SC.reconfigure :tight @14:50:29.77 SC.seek 4 @14:50:33.38 SC.configure :limp @14:50:33.59 Elbow.reconfigure :tight @14:50:33.64 Elbow.seek 4 @14:50:35.41 Forearm.reconfigure :tight @14:50:35.46 Forearm.seek 4 + 450 counts @14:50:36.48 SE.reconfigure :pick @14:50:36.54 SE.seek :beamBreak @14:50:38.32 SE.configure :default @14:50:38.49 Elbow.reconfigure :limp @14:50:38.54 Forearm.reconfigure :loose @14:50:38.59 Forearm.seek 4 @14:50:38.87 Elbow.hold @14:50:38.94 Elbow.reconfigure :tight @14:50:39.04 SE.seek 21.1 Pucks below Top Plate @14:50:39.59 Forearm.force -20 @14:50:39.63 Forearm.seek 4 + 302 counts @14:50:39.92 Forearm.reconfigure :tight @14:50:39.96 Forearm.seek 4 + 450 counts @14:50:40.16 SE.seek 22.1 Pucks below Top Plate @14:50:40.71 Forearm.reconfigure :loose @14:50:40.76 Forearm.seek :retract @14:50:42.17 Elbow.seek CC @14:50:43.88 Elbow.reconfigure :loose @14:50:43.98 Forearm.seek CC @14:50:45.00 Hand.close @14:50:45.64 CC.seek :open @14:50:50.38 Forearm.seek :retract @14:50:51.36 Elbow.reconfigure :tight @14:50:51.41 Elbow.seek 4 @14:50:53.16 Elbow.reconfigure :limp @14:50:53.21 Forearm.seek 4 @14:50:54.15 Elbow.hold @14:50:54.22 Elbow.reconfigure :tight @14:50:54.27 Hand.open @14:50:55.01 Forearm.force -20 @14:50:55.05 Forearm.seek 4 + 314 counts @14:50:55.46 Forearm.reconfigure :tight @14:50:55.51 Forearm.seek 4 + 450 counts @14:50:55.86 SE.seek :down @14:50:57.00
Forearm.reconfigure :loose @14:50:57.05 Forearm.seek :retract @14:50:57.10 SE.stop @14:50:58.12
PSV.dial :air @14:50:58.88 PS.seek 2.5ml @14:51:25.26 delay 1 second @14:51:26.28 PSV.dial :mixloop @14:51:26.74 delay 1 second @14:51:27.75 PSV.dial :air @14:51:28.22 delay 1 second @14:51:29.23 PSV.dial :mixloop @14:51:29.70 delay 1 second @14:51:30.72 PSV.dial :air @14:51:31.25 delay 1 second @14:51:32.27 PS.seek 0ml @14:51:58.64 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @14:51:58.88 Elbow.seek FlushPuck::Garage @14:52:00.74 Elbow.reconfigure :loose @14:52:00.85 Forearm.seek :garage @14:52:01.78 Hand.close @14:52:02.51 Forearm.seek :retract @14:52:03.49 Elbow.reconfigure :tight @14:52:03.53 Elbow.seek PC @14:52:04.77 Elbow.reconfigure :loose @14:52:04.87 Forearm.reconfigure :tight @14:52:04.92 Forearm.seek PC @14:52:06.19 PC.seek :closed @14:52:10.84 Processing Clamp onSpring/offSpring for 16/26 samples, on/off torque 1.61 @14:52:10.91 Hand.open @14:52:11.57 Forearm.reconfigure :loose @14:52:11.62 Forearm.seek :retract @14:52:12.62 PRV.dial :air @14:52:13.08 PSV.dial :mixloop @14:52:13.61 PS.seek 0.3ml @14:52:17.51 PSV.dial PSR @14:52:18.58 Pulling 0.4ml of PSR.air @14:52:18.59 PSR.select :air @14:52:18.61 PS.seek 0.7ml @14:52:23.99 delay 3 seconds @14:52:27.01 PSR.select :air @14:52:27.03 PS.seek 2.5ml @14:52:46.29 delay 3 seconds @14:52:49.31 PSV.dial :mixloop @14:52:50.37 PTV.dial :mixing @14:52:51.12 PBV.dial :waste2 @14:52:52.08 PS.seek 0ml @14:53:18.39 Garaging FlushPuck in Processing Clamp @14:53:18.46 Elbow.reconfigure :tight @14:53:18.51 Elbow.seek PC @14:53:18.63 Elbow.reconfigure :loose @14:53:18.73 Forearm.seek PC @14:53:19.70 Hand.close @14:53:20.27 PC.seek :open @14:53:24.97 Forearm.seek :retract @14:53:25.94 Elbow.reconfigure :tight @14:53:25.98 Elbow.seek :garage @14:53:27.32 Elbow.reconfigure :loose @14:53:27.42 Forearm.reconfigure :tight @14:53:27.46 Forearm.seek :garage @14:53:28.49 Hand.open @14:53:29.20 Forearm.reconfigure :loose @14:53:29.24 Forearm.seek :retract @14:53:30.29 Loading da puck from tube 5 into Processing Clamp @14:53:30.52 SC.reconfigure :tight @14:53:30.57 SC.seek 5 @14:53:34.13 SC.configure :limp @14:53:34.34 Elbow.reconfigure :tight @14:53:34.39 Elbow.seek 5 @14:53:36.24 Forearm.reconfigure :tight @14:53:36.29 Forearm.seek 5 + 450 counts @14:53:37.27 SE.reconfigure :pick @14:53:37.33 SE.seek :beamBreak @14:53:40.31 SE.configure :default @14:53:40.47 Elbow.seek 5 @14:53:40.65 Forearm.seek 5 + 300 counts @14:53:40.85 Forearm.reconfigure :loose @14:53:40.90 Hand.close @14:53:41.53 SE.seek :down @14:53:41.73
Forearm.seek :retract @14:53:42.73 Elbow.seek PC @14:53:43.69 SE.stop @14:53:45.33
Elbow.reconfigure :loose @14:53:45.43 Forearm.reconfigure :tight @14:53:45.48 Forearm.seek PC @14:53:46.70 PC.seek :closed @14:53:51.35 Processing Clamp onSpring/offSpring for 16/26 samples, on/off torque 1.66 @14:53:51.36 Hand.open @14:53:52.07 Forearm.reconfigure :loose @14:53:52.12 Forearm.seek :retract @14:53:53.10 Heating Puck.from 5, :da in Processing Clamp to 28C for 2:00:00 @14:53:53.12 PH.seek 28C,5:00 @14:54:37.96 PH.hold 2:00:00,28C @14:54:38.01 delay 1:30 @14:56:08.05 PSR.select :air @14:56:08.06 PSV.dial PSR @14:56:09.12 Pulling 0.5ml of PSR.wash @14:56:09.13 PSR.select :wash @14:56:09.25 PS.seek 0.5ml @14:56:15.16 delay 5 seconds @14:56:20.18 PSR.select :air @14:56:20.30 PS.seek 2ml @14:56:36.51 PTV.dial :puck @14:56:37.26 PSV.dial PTV @14:56:38.02 PBV.dial :waste1 @14:56:39.43 PS.seek 1.5ml @14:56:45.35 delay 3 seconds @14:56:48.37 PBV.dial between PSV and waste1 @14:56:48.72 PS.seek 0ml @14:57:04.85 delay 5:00 @15:02:04.87 recoverPSRreagent08 @15:02:04.88 PTV.dial :puck @15:02:04.95 PBV.dial :waste1 @15:02:05.32 delay 30 seconds @15:02:35.34 PBV.dial between PSV and waste1 @15:02:35.70 PSR.select :air @15:02:35.72 PSV.dial PSR @15:02:36.86 PS.reconfigure :fast @15:02:36.98 PS.seek 2.5ml @15:03:00.91 PSV.dial PTV @15:03:01.67 delay 5 seconds @15:03:06.69 PSV.dial PSR @15:03:07.47 delay 5 seconds @15:03:12.49 PSV.dial PTV @15:03:13.25 delay 5 seconds @15:03:18.27 PSV.dial :air @15:03:19.15 PS.seek 0ml @15:03:42.44 PTV.dial :PRVmixing,via: PRV @15:03:43.81 PSV.dial :mixloop @15:03:44.26 PRV.dial :ab1 @15:03:45.02 PS.reconfigure :slow @15:03:45.07 PS.seek 0.15ml @15:03:48.69 delay 2 seconds @15:03:50.71 CBV.dial PRV @15:03:50.73
PRV.dial CBV @15:03:51.31 PS.seek 0.35ml @15:03:55.91 delay 2 seconds @15:03:57.92 CBV.dial between PRV and PSV @15:03:58.25 PRV.dial :air @15:03:58.71 PS.seek 0.9ml @15:04:10.47 PRV.dial :waste2 @15:04:11.23 PS.seek 0.5ml @15:04:19.97 addPMLReagent08 @15:04:19.98 PSV.dial :air @15:04:20.44 PS.reconfigure :default @15:04:20.49 PS.seek 2ml @15:04:36.72 PSV.dial :PML @15:04:37.19 PTV.dial :mixing @15:04:37.94 PBV.dial :waste1 @15:04:38.37 PS.seek 1.85ml @15:04:40.69 PBV.dial between PSV and waste1 @15:04:41.07 PS.seek 0ml @15:05:00.81 delay 2 seconds @15:05:02.83 Beginning samplePassC @15:05:02.84 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @15:05:03.03 Elbow.reconfigure :tight @15:05:03.08 Elbow.seek FlushPuck::Garage @15:05:04.54 Elbow.reconfigure :loose @15:05:04.64 Forearm.seek :garage @15:05:05.60 Hand.close @15:05:06.81 Forearm.seek :retract @15:05:07.77 Elbow.reconfigure :tight @15:05:07.82 Elbow.seek CC @15:05:10.75 Elbow.reconfigure :loose @15:05:10.85 Forearm.reconfigure :tight @15:05:10.90 Forearm.seek CC @15:05:12.12 CC.seek :closed @15:05:16.63 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.46 @15:05:16.70 Hand.open @15:05:17.31 Forearm.reconfigure :loose @15:05:17.36 Forearm.seek :retract @15:05:18.31 PSV.dial PSR @15:05:19.37 Pulling 1.5ml of PSR.flush @15:05:19.38 PSR.select :flush @15:05:19.51 PS.seek 1.5ml @15:05:35.65 delay 5 seconds @15:05:40.67 PSV.dial :waste1 @15:05:41.14 PS.seek 0ml @15:05:57.25 PSV.dial PSR @15:05:57.72 PSR.select :air @15:05:57.83 PS.seek 2.5ml @15:06:24.13 PRV.dial CBV,avoiding: :ab2 @15:06:24.17
CBV.dial PRV @15:06:25.76 PSV.dial :mixloop @15:06:26.81 CTV.dial :air,avoiding: :intake @15:06:27.04 PTV.dial :PRVmixing @15:06:27.82 PS.seek 0ml @15:06:54.19 PSV.dial :air @15:06:54.66 PS.seek 2.5ml @15:07:21.47 PSV.dial :mixloop @15:07:22.00 PS.seek 0ml @15:07:48.37 PSV.dial :air @15:07:48.85 PS.seek 2.5ml @15:08:15.24 PSV.dial :mixloop @15:08:15.72 PS.seek 0ml @15:08:42.06 Garaging FlushPuck in Collection Clamp @15:08:42.14 Elbow.reconfigure :tight @15:08:42.18 Elbow.seek CC @15:08:42.46 Elbow.reconfigure :loose @15:08:42.56 Forearm.seek CC @15:08:43.62 Hand.close @15:08:44.25 CC.seek :open @15:08:48.86 Forearm.seek :retract @15:08:49.85 Elbow.reconfigure :tight @15:08:49.89 Elbow.seek :garage @15:08:52.82 Elbow.reconfigure :loose @15:08:52.92 Forearm.reconfigure :tight @15:08:52.97 Forearm.seek :garage @15:08:53.97 Hand.open @15:08:54.68 Forearm.reconfigure :loose @15:08:54.73 Forearm.seek :retract @15:08:55.72 delayUntil "15:13:02.82" @15:08:55.75 Whole Cell Archive sampling 1000ml @15:08:55.76 Loading wcr puck from tube 5 into Collection Clamp @15:08:56.24 Elbow.reconfigure :tight @15:08:56.29 Elbow.seek 5 @15:08:58.32 Forearm.reconfigure :tight @15:08:58.38 Forearm.seek 5 + 450 counts @15:08:59.83 SE.reconfigure :pick @15:08:59.90 SE.seek :beamBreak @15:09:02.45 SE.configure :default @15:09:02.62 Elbow.seek 5 @15:09:03.17 Forearm.seek 5 + 300 counts @15:09:03.39 Forearm.reconfigure :loose @15:09:03.44 Hand.close @15:09:04.01 SE.seek :down @15:09:04.22 Forearm.seek :retract @15:09:05.20 Elbow.seek CC @15:09:06.23 SE.stop @15:09:06.85 Elbow.reconfigure :loose @15:09:06.96 Forearm.reconfigure :tight @15:09:07.01 Forearm.seek CC @15:09:08.23 CC.seek :closed @15:09:12.75 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.33 @15:09:12.77 Hand.open @15:09:13.43 Forearm.reconfigure :loose @15:09:13.48 Forearm.seek :retract @15:09:14.44 collectSample 1000ml w/bubble point of 35psi @15:09:14.45 Priming 7ml @15:09:14.46 Intake.select :close @15:09:14.47 Exhaust.select :close @15:09:14.49 SS.hold @15:09:15.11 Calibrating pressure sensors @15:09:15.16 CTV.dial :air @15:09:15.23 SSV.dial :spit,avoiding: :exhaust @15:09:15.34 SS.reconfigure :fast @15:09:15.39 SS.seek 5ml @15:09:18.13 SSV.dial CBV @15:09:18.15 CBV.dial SSV @15:09:19.53 delay 3.8 seconds @15:09:23.47 Sensor limits: Intake max 102.4psia; Outlet max 93.9psia @15:09:23.48 SSV.dial :spit @15:09:24.82 SS.seek 0ml @15:09:27.61 SSV.dial CBV @15:09:28.94 CTV.dial :intake @15:09:29.73 SSV.dial CBV @15:09:29.76 CBV.dial SSV @15:09:29.84 Allowing 40s for 7.0ml of remaining 7.0ml @15:09:29.85 Intake.select :open @15:09:32.03 Intake @14.4psia, vacuum from 8.9 to 11.9psi @15:09:32.04 SS.reconfigure :inhaling 2.20ml/s @15:09:32.09 SS.seek 7ml @15:09:38.84 Stroke completed -- waiting for pressure to equalize ... @15:09:38.85 SS.reconfigure :exhale @15:09:38.90 SS.hold @15:09:53.30 Intake.select :close @15:09:53.46 SSV.dial :exhaust @15:09:54.22 Exhaust.select :open @15:09:54.34 SS.seek 0ml @15:09:58.48 Exhaust.select :close @15:09:58.59 Sampling 1000.0ml @15:09:58.61 Intake.select :close @15:09:58.62 Exhaust.select :close @15:09:58.63 SS.reconfigure :default @15:09:58.67 SS.hold @15:09:58.89 CTV.dial :intake @15:09:58.97 SSV.dial CBV @15:09:58.99 CBV.dial SSV @15:09:59.79 Allowing 130s for 25.0ml of remaining 1000.0ml @15:09:59.81 Intake.select :open @15:10:01.98 Intake @15.3psia, vacuum from 9.8 to 12.8psi @15:10:02.00 SS.reconfigure :inhaling 2.20ml/s @15:10:02.04 SS.seek 25ml @15:10:09.79 Vacuum too high -- waiting up to 40 seconds @15:10:09.80 SS.reconfigure :exhale @15:10:09.86 SS.hold @15:10:17.21 Intake @15.6psia, vacuum from 10.0 to 13.0psi @15:10:17.23 SS.reconfigure :inhaling 1.76ml/s @15:10:17.28 SS.seek 25ml @15:10:24.19 Vacuum too high -- waiting up to 40 seconds @15:10:24.21 SS.reconfigure :exhale @15:10:24.26 SS.hold @15:10:34.37 Intake @19.3psia, vacuum from 10.0 to 13.0psi @15:10:34.40 SS.reconfigure :inhaling 1.41ml/s @15:10:34.44 SS.seek 25ml @15:10:37.19 Vacuum too high -- waiting up to 40 seconds @15:10:37.21 SS.reconfigure :exhale @15:10:37.26 SS.hold @15:10:38.27 Intake @15.7psia, vacuum from 10.0 to 13.0psi @15:10:38.31 SS.reconfigure :inhaling 1.13ml/s @15:10:38.36 SS.seek 25ml @15:10:40.21 Stroke completed -- waiting for pressure to equalize ... @15:10:40.23 SS.reconfigure :exhale @15:10:40.28 SS.hold @15:11:08.89 Intake.select :close @15:11:09.05 SSV.dial :exhaust @15:11:09.83 Exhaust.select :open @15:11:09.94 SS.seek 0ml @15:11:18.79 Exhaust.select :close @15:11:18.90 SSV.dial CBV @15:11:18.93 CBV.dial SSV @15:11:19.73 Allowing 130s for 25.0ml of remaining 975.0ml @15:11:19.74 Intake.select :open @15:11:21.92 Intake @15.9psia, vacuum from 10.0 to 13.0psi @15:11:21.93 SS.reconfigure :inhaling 1.13ml/s @15:11:21.98 SS.seek 25ml @15:11:42.75 Vacuum too high -- waiting up to 40 seconds @15:11:42.77 SS.reconfigure :exhale @15:11:42.84 SS.hold @15:11:52.74 Intake @19.3psia, vacuum from 10.0 to 13.0psi @15:11:52.77 SS.reconfigure :inhaling 0.91ml/s @15:11:53.27 SS.seek 25ml @15:11:57.79 Vacuum too high -- waiting up to 40 seconds @15:11:57.81 SS.reconfigure :exhale @15:11:57.86 SS.hold @15:12:00.46 Intake @19.1psia, vacuum from 10.0 to 13.0psi @15:12:00.47 SS.reconfigure :inhaling 0.73ml/s @15:12:00.52 SS.seek 25ml @15:12:01.85 Stroke completed -- waiting for pressure to equalize ... @15:12:01.87 SS.reconfigure :exhale @15:12:01.91 SS.hold @15:12:28.96 Intake.select :close @15:12:29.12 SSV.dial :exhaust @15:12:29.89 Exhaust.select :open @15:12:30.00 SS.seek 0ml @15:12:39.04 Exhaust.select :close @15:12:39.16 SSV.dial CBV @15:12:39.20 CBV.dial SSV @15:12:39.95 Allowing 130s for 25.0ml of remaining 950.0ml @15:12:39.96 Intake.select :open @15:12:42.13 Intake @15.9psia, vacuum from 10.0 to 13.0psi @15:12:42.15 SS.reconfigure :inhaling 0.73ml/s @15:12:42.20 SS.seek 25ml @15:13:02.83 recoverPRVreagent08 @15:13:02.85 PTV.dial :mixing @15:13:03.61 PBV.dial :waste1 @15:13:03.99 delay 30 seconds @15:13:18.32 Stroke completed -- waiting for pressure to equalize ... @15:13:18.33 SS.reconfigure :exhale @15:13:18.38 SS.hold @15:13:34.01 PBV.dial between PSV and waste1 @15:13:34.38 PSV.dial :air @15:13:34.85 PS.reconfigure :fast @15:13:34.90 PS.seek 2.5ml @15:13:49.12 Intake.select :close @15:13:49.28 SSV.dial :exhaust @15:13:50.04 Exhaust.select :open @15:13:50.16 SS.seek 0ml @15:13:58.96 PSV.dial :mixloop @15:13:59.02 Exhaust.select :close @15:13:59.13 SSV.dial CBV @15:13:59.15 CBV.dial SSV @15:13:59.73 delay 2 seconds @15:14:00.46 Allowing 130s for 25.0ml of remaining 925.0ml @15:14:00.48 Intake.select :open @15:14:01.76 PSV.dial :air @15:14:02.22 delay 2 seconds @15:14:02.65 Intake @16.0psia, vacuum from 10.0 to 13.0psi @15:14:02.67 SS.reconfigure :inhaling 0.73ml/s @15:14:02.72 SS.seek 25ml @15:14:04.24 PSV.dial :mixloop @15:14:04.72 delay 2 seconds @15:14:06.74 PSV.dial :air @15:14:07.21 PS.seek 0ml @15:14:30.49 washCycleDA #1 of 2 @15:14:30.50 PSR.select :air @15:14:30.52 PSV.dial PSR @15:14:31.86 Pulling 0.5ml of PSR.wash @15:14:31.87 PSR.select :wash @15:14:31.99 PS.reconfigure :default @15:14:32.04 PS.seek 0.5ml @15:14:38.02 delay 5 seconds @15:14:39.30 Stroke completed -- waiting for pressure to equalize ... @15:14:39.32 SS.reconfigure :exhale @15:14:39.37 SS.hold @15:14:43.03 PSR.select :air @15:14:43.16 PS.seek 2ml @15:14:59.33 PTV.dial :puck @15:15:00.08 PSV.dial PTV @15:15:00.84 PBV.dial :waste1 @15:15:01.27 PS.seek 1.5ml @15:15:07.14 delay 3 seconds @15:15:07.96 Intake.select :close @15:15:08.12 SSV.dial :exhaust @15:15:08.90 Exhaust.select :open @15:15:09.02 SS.seek 0ml @15:15:10.16 PBV.dial between PSV and waste1 @15:15:10.54 PS.seek 0ml @15:15:18.04 Exhaust.select :close @15:15:18.15 SSV.dial CBV @15:15:18.17 CBV.dial SSV @15:15:19.01 Allowing 130s for 25.0ml of remaining 900.0ml @15:15:19.02 Intake.select :open @15:15:21.19 Intake @16.1psia, vacuum from 10.0 to 13.0psi @15:15:21.21 SS.reconfigure :inhaling 0.73ml/s @15:15:21.26 SS.seek 25ml @15:15:26.68 delay 2:00 @15:15:57.19 Stroke completed -- waiting for pressure to equalize ... @15:15:57.20 SS.reconfigure :exhale @15:15:57.25 SS.hold @15:16:23.99 Intake.select :close @15:16:24.14 SSV.dial :exhaust @15:16:24.91 Exhaust.select :open @15:16:25.03 SS.seek 0ml @15:16:34.05 Exhaust.select :close @15:16:34.16 SSV.dial CBV @15:16:34.18 CBV.dial SSV @15:16:34.99 Allowing 130s for 25.0ml of remaining 875.0ml @15:16:35.00 Intake.select :open @15:16:37.17 Intake @16.2psia, vacuum from 10.0 to 13.0psi @15:16:37.19 SS.reconfigure :inhaling 0.73ml/s @15:16:37.70 SS.seek 25ml @15:17:13.58 Stroke completed -- waiting for pressure to equalize ... @15:17:13.59 SS.reconfigure :exhale @15:17:13.64 SS.hold @15:17:26.69 recoverPSRreagent08 @15:17:26.71 PTV.dial :puck @15:17:26.78 PBV.dial :waste1 @15:17:27.16 delay 30 seconds @15:17:40.23 Intake.select :close @15:17:40.39 SSV.dial :exhaust @15:17:41.18 Exhaust.select :open @15:17:41.30 SS.seek 0ml @15:17:50.25 Exhaust.select :close @15:17:50.36 SSV.dial CBV @15:17:50.39 CBV.dial SSV @15:17:51.19 Allowing 130s for 25.0ml of remaining 850.0ml @15:17:51.21 Intake.select :open @15:17:53.37 Intake @16.1psia, vacuum from 10.0 to 13.0psi @15:17:53.39 SS.reconfigure :inhaling 0.73ml/s @15:17:53.44 SS.seek 25ml @15:17:57.17 PBV.dial between PSV and waste1 @15:17:57.53 PSR.select :air @15:17:57.55 PSV.dial PSR @15:17:58.44 PS.reconfigure :fast @15:17:58.49 PS.seek 2.5ml @15:18:22.45 PSV.dial PTV @15:18:23.20 delay 5 seconds @15:18:28.22 PSV.dial PSR @15:18:28.98 delay 5 seconds @15:18:29.45 Stroke completed -- waiting for pressure to equalize ... @15:18:29.47 SS.reconfigure :exhale @15:18:29.52 SS.hold @15:18:34.00 PSV.dial PTV @15:18:34.76 delay 5 seconds @15:18:39.78 PSV.dial :air @15:18:40.55 PS.seek 0ml @15:18:56.53 Intake.select :close @15:18:56.68 SSV.dial :exhaust @15:18:57.45 Exhaust.select :open @15:18:57.57 SS.seek 0ml @15:19:03.99 washCycleDA #2 of 2 @15:19:04.00 PSR.select :air @15:19:04.01 PSV.dial PSR @15:19:05.36 Pulling 0.5ml of PSR.wash @15:19:05.37 PSR.select :wash @15:19:05.49 PS.reconfigure :default @15:19:05.53 PS.seek 0.5ml @15:19:06.39 Exhaust.select :close @15:19:06.50 SSV.dial CBV @15:19:06.52 CBV.dial SSV @15:19:07.35 Allowing 130s for 25.0ml of remaining 825.0ml @15:19:07.36 Intake.select :open @15:19:09.54 Intake @15.9psia, vacuum from 10.0 to 13.0psi @15:19:09.56 SS.reconfigure :inhaling 0.73ml/s @15:19:09.61 SS.seek 25ml @15:19:11.51 delay 5 seconds @15:19:16.53 PSR.select :air @15:19:16.65 PS.seek 2ml @15:19:32.85 PTV.dial :puck @15:19:32.93 PSV.dial PTV @15:19:33.81 PBV.dial :waste1 @15:19:34.70 PS.seek 1.5ml @15:19:40.64 delay 3 seconds @15:19:43.66 PBV.dial between PSV and waste1 @15:19:44.03 PS.seek 0ml @15:19:46.12 Stroke completed -- waiting for pressure to equalize ... @15:19:46.14 SS.reconfigure :exhale @15:19:46.19 SS.hold @15:20:00.14 delay 2:00 @15:20:11.16 Intake.select :close @15:20:11.32 SSV.dial :exhaust @15:20:12.08 Exhaust.select :open @15:20:12.20 SS.seek 0ml @15:20:21.03 Exhaust.select :close @15:20:21.15 SSV.dial CBV @15:20:21.17 CBV.dial SSV @15:20:21.99 Allowing 130s for 25.0ml of remaining 800.0ml @15:20:22.00 Intake.select :open @15:20:24.17 Intake @16.1psia, vacuum from 10.0 to 13.0psi @15:20:24.19 SS.reconfigure :inhaling 0.73ml/s @15:20:24.24 SS.seek 25ml @15:20:58.56 Vacuum too high -- waiting up to 40 seconds @15:20:58.58 SS.reconfigure :exhale @15:20:58.64 SS.hold @15:21:06.25 Intake @16.2psia, vacuum from 10.0 to 13.0psi @15:21:06.26 SS.reconfigure :inhaling 0.59ml/s @15:21:06.31 SS.seek 25ml @15:21:09.14 Stroke completed -- waiting for pressure to equalize ... @15:21:09.15 SS.reconfigure :exhale @15:21:09.20 SS.hold @15:21:25.97 Intake.select :close @15:21:26.12 SSV.dial :exhaust @15:21:26.89 Exhaust.select :open @15:21:27.01 SS.seek 0ml @15:21:35.98 Exhaust.select :close @15:21:36.09 SSV.dial CBV @15:21:36.11 CBV.dial SSV @15:21:36.92 Allowing 130s for 25.0ml of remaining 775.0ml @15:21:36.93 Intake.select :open @15:21:39.10 Intake @16.1psia, vacuum from 10.0 to 13.0psi @15:21:39.12 SS.reconfigure :inhaling 0.59ml/s @15:21:39.17 SS.seek 25ml @15:22:00.17 recoverPSRreagent08 @15:22:00.18 PTV.dial :puck @15:22:00.71 PBV.dial :waste1 @15:22:01.09 delay 30 seconds @15:22:23.59 Stroke completed -- waiting for pressure to equalize ... @15:22:23.60 SS.reconfigure :exhale @15:22:23.65 SS.hold @15:22:31.11 PBV.dial between PSV and waste1 @15:22:31.48 PSR.select :air @15:22:31.50 PSV.dial PSR @15:22:32.26 PS.reconfigure :fast @15:22:32.31 PS.seek 2.5ml @15:22:41.92 Intake.select :close @15:22:42.07 SSV.dial :exhaust @15:22:42.93 Exhaust.select :open @15:22:43.05 SS.seek 0ml @15:22:51.84 Exhaust.select :close @15:22:51.95 SSV.dial CBV @15:22:51.97 CBV.dial SSV @15:22:52.76 Allowing 130s for 25.0ml of remaining 750.0ml @15:22:52.78 Intake.select :open @15:22:54.95 Intake @16.1psia, vacuum from 10.0 to 13.0psi @15:22:54.97 SS.reconfigure :inhaling 0.59ml/s @15:22:55.02 SS.seek 25ml @15:22:56.29 PSV.dial PTV @15:22:57.18 delay 5 seconds @15:23:02.20 PSV.dial PSR @15:23:02.96 delay 5 seconds @15:23:07.98 PSV.dial PTV @15:23:08.74 delay 5 seconds @15:23:13.76 PSV.dial :air @15:23:14.55 PS.seek 0ml @15:23:37.79 getPMLReagent08 @15:23:37.80 PRV.dial :air @15:23:38.26 delay 5 seconds @15:23:39.71 Stroke completed -- waiting for pressure to equalize ... @15:23:39.73 SS.reconfigure :exhale @15:23:39.77 SS.hold @15:23:43.33 PTV.dial :PRVmixing @15:23:44.68 delay 5 seconds @15:23:49.69 PRV.dial :ab2 @15:23:50.16 PTV.dial :PRVmixing @15:23:50.24 PSV.dial :mixloop @15:23:50.72 PS.reconfigure :default @15:23:50.77 PS.seek 0.3ml @15:23:54.60 delay 2 seconds @15:23:56.62 PRV.dial :air @15:23:57.16 PS.seek 0.55ml @15:24:00.20 Intake.select :close @15:24:00.36 SSV.dial :exhaust @15:24:00.51 addPMLReagent08 @15:24:00.52 PSV.dial :air @15:24:01.00 PS.seek 2ml @15:24:01.14 Exhaust.select :open @15:24:01.26 SS.seek 0ml @15:24:10.48 Exhaust.select :close @15:24:10.59 SSV.dial CBV @15:24:10.63 CBV.dial SSV @15:24:11.39 Allowing 130s for 25.0ml of remaining 725.0ml @15:24:11.40 Intake.select :open @15:24:13.57 Intake @16.1psia, vacuum from 10.0 to 13.0psi @15:24:13.59 SS.reconfigure :inhaling 0.59ml/s @15:24:13.64 SS.seek 25ml @15:24:16.66 PSV.dial :PML @15:24:17.13 PTV.dial :mixing @15:24:17.90 PBV.dial :waste1 @15:24:18.33 PS.seek 1.85ml @15:24:20.76 PBV.dial between PSV and waste1 @15:24:21.14 PS.seek 0ml @15:24:41.10 delay 2 seconds @15:24:43.12 delay 8:00 @15:24:58.06 Stroke completed -- waiting for pressure to equalize ... @15:24:58.07 SS.reconfigure :exhale @15:24:58.12 SS.hold @15:25:21.06 Intake.select :close @15:25:21.21 SSV.dial :exhaust @15:25:21.99 Exhaust.select :open @15:25:22.11 SS.seek 0ml @15:25:30.93 Exhaust.select :close @15:25:31.05 SSV.dial CBV @15:25:31.08 CBV.dial SSV @15:25:31.87 Allowing 130s for 25.0ml of remaining 700.0ml @15:25:31.88 Intake.select :open @15:25:34.53 Intake @16.1psia, vacuum from 10.0 to 13.0psi @15:25:34.55 SS.reconfigure :inhaling 0.59ml/s @15:25:34.60 SS.seek 25ml @15:26:19.26 Stroke completed -- waiting for pressure to equalize ... @15:26:19.27 SS.reconfigure :exhale @15:26:19.32 SS.hold @15:26:39.64 Intake.select :close @15:26:39.80 SSV.dial :exhaust @15:26:40.58 Exhaust.select :open @15:26:41.17 SS.seek 0ml @15:26:50.18 Exhaust.select :close @15:26:50.29 SSV.dial CBV @15:26:50.34 CBV.dial SSV @15:26:51.09 Allowing 130s for 25.0ml of remaining 675.0ml @15:26:51.10 Intake.select :open @15:26:53.27 Intake @16.1psia, vacuum from 10.0 to 13.0psi @15:26:53.29 SS.reconfigure :inhaling 0.59ml/s @15:26:53.34 SS.seek 25ml @15:27:37.88 Stroke completed -- waiting for pressure to equalize ... @15:27:37.89 SS.reconfigure :exhale @15:27:37.94 SS.hold @15:28:02.97 Intake.select :close @15:28:03.13 SSV.dial :exhaust @15:28:03.90 Exhaust.select :open @15:28:04.02 SS.seek 0ml @15:28:12.88 Exhaust.select :close @15:28:13.00 SSV.dial CBV @15:28:13.02 CBV.dial SSV @15:28:13.82 Allowing 130s for 25.0ml of remaining 650.0ml @15:28:13.83 Intake.select :open @15:28:16.00 Intake @16.0psia, vacuum from 10.0 to 13.0psi @15:28:16.02 SS.reconfigure :inhaling 0.59ml/s @15:28:16.07 SS.seek 25ml @15:28:55.89 Vacuum too high -- waiting up to 40 seconds @15:28:55.91 SS.reconfigure :exhale @15:28:55.96 SS.hold @15:29:07.24 Intake @17.5psia, vacuum from 10.0 to 13.0psi @15:29:07.26 SS.reconfigure :inhaling 0.47ml/s @15:29:07.31 SS.seek 25ml @15:29:13.85 Stroke completed -- waiting for pressure to equalize ... @15:29:13.86 SS.reconfigure :exhale @15:29:13.91 SS.hold @15:29:28.30 Intake.select :close @15:29:28.46 SSV.dial :exhaust @15:29:29.24 Exhaust.select :open @15:29:29.36 SS.seek 0ml @15:29:38.61 Exhaust.select :close @15:29:38.72 SSV.dial CBV @15:29:38.74 CBV.dial SSV @15:29:39.54 Allowing 130s for 25.0ml of remaining 625.0ml @15:29:39.55 Intake.select :open @15:29:41.72 Intake @16.2psia, vacuum from 10.0 to 13.0psi @15:29:41.74 SS.reconfigure :inhaling 0.47ml/s @15:29:41.79 SS.seek 25ml @15:30:37.41 Stroke completed -- waiting for pressure to equalize ... @15:30:37.42 SS.reconfigure :exhale @15:30:37.47 SS.hold @15:30:57.94 Intake.select :close @15:30:58.10 SSV.dial :exhaust @15:30:58.88 Exhaust.select :open @15:30:58.99 SS.seek 0ml @15:31:07.80 Exhaust.select :close @15:31:07.91 SSV.dial CBV @15:31:07.95 CBV.dial SSV @15:31:08.72 Allowing 130s for 25.0ml of remaining 600.0ml @15:31:08.74 Intake.select :open @15:31:10.90 Intake @16.2psia, vacuum from 10.0 to 13.0psi @15:31:10.92 SS.reconfigure :inhaling 0.47ml/s @15:31:10.97 SS.seek 25ml @15:32:06.36 Stroke completed -- waiting for pressure to equalize ... @15:32:06.37 SS.reconfigure :exhale @15:32:06.42 SS.hold @15:32:29.35 Intake.select :close @15:32:29.51 SSV.dial :exhaust @15:32:30.27 Exhaust.select :open @15:32:30.39 SS.seek 0ml @15:32:39.22 Exhaust.select :close @15:32:39.34 SSV.dial CBV @15:32:39.36 CBV.dial SSV @15:32:40.17 Allowing 130s for 25.0ml of remaining 575.0ml @15:32:40.19 Intake.select :open @15:32:42.35 Intake @16.1psia, vacuum from 10.0 to 13.0psi @15:32:42.37 SS.reconfigure :inhaling 0.47ml/s @15:32:42.42 SS.seek 25ml @15:32:43.14 recoverPRVreagent08 @15:32:43.15 PTV.dial :mixing @15:32:43.31 PBV.dial :waste1 @15:32:43.69 delay 30 seconds @15:33:13.71 PBV.dial between PSV and waste1 @15:33:14.07 PSV.dial :air @15:33:14.55 PS.reconfigure :fast @15:33:14.60 PS.seek 2.5ml @15:33:37.92 Stroke completed -- waiting for pressure to equalize ... @15:33:37.93 SS.reconfigure :exhale @15:33:37.98 SS.hold @15:33:38.89 PSV.dial :mixloop @15:33:39.39 delay 2 seconds @15:33:41.40 PSV.dial :air @15:33:42.33 delay 2 seconds @15:33:44.34 PSV.dial :mixloop @15:33:44.81 delay 2 seconds @15:33:46.83 PSV.dial :air @15:33:47.30 PS.seek 0ml @15:34:03.04 Intake.select :close @15:34:03.20 SSV.dial :exhaust @15:34:03.97 Exhaust.select :open @15:34:04.09 SS.seek 0ml @15:34:10.72 washCycleDA #1 of 3 @15:34:10.73 PSR.select :air @15:34:10.74 PSV.dial PSR @15:34:12.09 Pulling 0.5ml of PSR.wash @15:34:12.10 PSR.select :wash @15:34:12.22 PS.reconfigure :default @15:34:12.27 PS.seek 0.5ml @15:34:13.14 Exhaust.select :close @15:34:13.25 SSV.dial CBV @15:34:13.29 CBV.dial SSV @15:34:14.05 Allowing 130s for 25.0ml of remaining 550.0ml @15:34:14.06 Intake.select :open @15:34:16.23 Intake @16.1psia, vacuum from 10.0 to 13.0psi @15:34:16.25 SS.reconfigure :inhaling 0.47ml/s @15:34:16.77 SS.seek 25ml @15:34:18.25 delay 5 seconds @15:34:23.26 PSR.select :air @15:34:23.38 PS.seek 2ml @15:34:39.59 PTV.dial :puck @15:34:40.35 PSV.dial PTV @15:34:41.10 PBV.dial :waste1 @15:34:41.54 PS.seek 1.5ml @15:34:47.53 delay 3 seconds @15:34:50.55 PBV.dial between PSV and waste1 @15:34:50.92 PS.seek 0ml @15:35:07.09 delay 2:00 @15:35:11.90 Stroke completed -- waiting for pressure to equalize ... @15:35:11.92 SS.reconfigure :exhale @15:35:11.96 SS.hold @15:35:41.06 Intake.select :close @15:35:41.22 SSV.dial :exhaust @15:35:42.00 Exhaust.select :open @15:35:42.12 SS.seek 0ml @15:35:50.98 Exhaust.select :close @15:35:51.10 SSV.dial CBV @15:35:51.12 CBV.dial SSV @15:35:51.90 Allowing 130s for 25.0ml of remaining 525.0ml @15:35:51.92 Intake.select :open @15:35:54.08 Intake @16.1psia, vacuum from 10.0 to 13.0psi @15:35:54.10 SS.reconfigure :inhaling 0.47ml/s @15:35:54.15 SS.seek 25ml @15:36:45.82 Vacuum too high -- waiting up to 40 seconds @15:36:45.83 SS.reconfigure :exhale @15:36:45.89 SS.hold @15:37:00.40 Intake @17.8psia, vacuum from 10.0 to 13.0psi @15:37:00.42 SS.reconfigure :inhaling 0.38ml/s @15:37:00.47 SS.seek 25ml @15:37:05.81 Stroke completed -- waiting for pressure to equalize ... @15:37:05.82 SS.reconfigure :exhale @15:37:05.87 SS.hold @15:37:07.14 recoverPSRreagent08 @15:37:07.15 PTV.dial :puck @15:37:07.23 PBV.dial :waste1 @15:37:07.60 delay 30 seconds @15:37:22.31 Intake.select :close @15:37:22.47 SSV.dial :exhaust @15:37:23.23 Exhaust.select :open @15:37:23.35 SS.seek 0ml @15:37:32.37 Exhaust.select :close @15:37:32.48 SSV.dial CBV @15:37:32.51 CBV.dial SSV @15:37:33.32 Allowing 130s for 25.0ml of remaining 500.0ml @15:37:33.34 Intake.select :open @15:37:35.50 Intake @16.1psia, vacuum from 10.0 to 13.0psi @15:37:35.52 SS.reconfigure :inhaling 0.38ml/s @15:37:35.57 SS.seek 25ml @15:37:37.62 PBV.dial between PSV and waste1 @15:37:38.01 PSR.select :air @15:37:38.03 PSV.dial PSR @15:37:38.92 PS.reconfigure :fast @15:37:38.99 PS.seek 2.5ml @15:38:03.11 PSV.dial PTV @15:38:03.87 delay 5 seconds @15:38:08.89 PSV.dial PSR @15:38:09.65 delay 5 seconds @15:38:14.67 PSV.dial PTV @15:38:15.57 delay 5 seconds @15:38:20.59 PSV.dial :air @15:38:21.37 PS.seek 0ml @15:38:43.35 Stroke completed -- waiting for pressure to equalize ... @15:38:43.37 SS.reconfigure :exhale @15:38:43.41 SS.hold @15:38:44.87 washCycleDA #2 of 3 @15:38:44.88 PSR.select :air @15:38:44.89 PSV.dial PSR @15:38:46.41 Pulling 0.5ml of PSR.wash @15:38:46.42 PSR.select :wash @15:38:46.62 PS.reconfigure :default @15:38:46.67 PS.seek 0.5ml @15:38:52.58 delay 5 seconds @15:38:57.60 PSR.select :air @15:38:57.72 PS.seek 2ml @15:39:12.22 Intake.select :close @15:39:12.38 SSV.dial :exhaust @15:39:13.15 Exhaust.select :open @15:39:13.27 SS.seek 0ml @15:39:13.89 PTV.dial :puck @15:39:13.97 PSV.dial PTV @15:39:14.72 PBV.dial :waste1 @15:39:15.16 PS.seek 1.5ml @15:39:21.56 delay 3 seconds @15:39:22.13 Exhaust.select :close @15:39:22.24 SSV.dial CBV @15:39:22.29 CBV.dial SSV @15:39:23.04 Allowing 130s for 25.0ml of remaining 475.0ml @15:39:23.05 Intake.select :open @15:39:24.58 PBV.dial between PSV and waste1 @15:39:24.96 PS.seek 0ml @15:39:25.22 Intake @16.2psia, vacuum from 10.0 to 13.0psi @15:39:25.24 SS.reconfigure :inhaling 0.38ml/s @15:39:25.29 SS.seek 25ml @15:39:41.08 delay 2:00 @15:40:33.90 Stroke completed -- waiting for pressure to equalize ... @15:40:33.91 SS.reconfigure :exhale @15:40:33.96 SS.hold @15:41:04.61 Intake.select :close @15:41:04.77 SSV.dial :exhaust @15:41:05.54 Exhaust.select :open @15:41:05.66 SS.seek 0ml @15:41:14.77 Exhaust.select :close @15:41:14.88 SSV.dial CBV @15:41:14.91 CBV.dial SSV @15:41:15.72 Allowing 130s for 25.0ml of remaining 450.0ml @15:41:15.74 Intake.select :open @15:41:17.91 Intake @16.3psia, vacuum from 10.0 to 13.0psi @15:41:17.93 SS.reconfigure :inhaling 0.38ml/s @15:41:17.98 SS.seek 25ml @15:41:41.10 recoverPSRreagent08 @15:41:41.11 PTV.dial :puck @15:41:41.19 PBV.dial :waste1 @15:41:41.57 delay 30 seconds @15:42:11.59 PBV.dial between PSV and waste1 @15:42:11.95 PSR.select :air @15:42:11.98 PSV.dial PSR @15:42:12.74 PS.reconfigure :fast @15:42:12.79 PS.seek 2.5ml @15:42:25.89 Stroke completed -- waiting for pressure to equalize ... @15:42:25.91 SS.reconfigure :exhale @15:42:25.95 SS.hold @15:42:36.64 PSV.dial PTV @15:42:37.41 delay 5 seconds @15:42:42.42 PSV.dial PSR @15:42:43.18 delay 5 seconds @15:42:48.19 PSV.dial PTV @15:42:48.96 delay 5 seconds @15:42:53.97 PSV.dial :air @15:42:54.74 PS.seek 0ml @15:43:00.79 Intake.select :close @15:43:00.95 SSV.dial :exhaust @15:43:02.12 Exhaust.select :open @15:43:02.31 SS.seek 0ml @15:43:11.49 Exhaust.select :close @15:43:11.60 SSV.dial CBV @15:43:11.63 CBV.dial SSV @15:43:12.43 Allowing 130s for 25.0ml of remaining 425.0ml @15:43:12.44 Intake.select :open @15:43:14.61 Intake @16.2psia, vacuum from 10.0 to 13.0psi @15:43:14.63 SS.reconfigure :inhaling 0.38ml/s @15:43:14.68 SS.seek 25ml @15:43:18.52 washCycleDA #3 of 3 @15:43:18.53 PSR.select :air @15:43:18.55 PSV.dial PSR @15:43:19.89 Pulling 0.5ml of PSR.wash @15:43:19.90 PSR.select :wash @15:43:20.02 PS.reconfigure :default @15:43:20.07 PS.seek 0.5ml @15:43:26.08 delay 5 seconds @15:43:31.10 PSR.select :air @15:43:31.22 PS.seek 2ml @15:43:47.30 PTV.dial :puck @15:43:47.37 PSV.dial PTV @15:43:48.12 PBV.dial :waste1 @15:43:48.56 PS.seek 1.5ml @15:43:54.48 delay 3 seconds @15:43:57.50 PBV.dial between PSV and waste1 @15:43:57.88 PS.seek 0ml @15:44:14.27 delay 2:00 @15:44:22.39 Stroke completed -- waiting for pressure to equalize ... @15:44:22.40 SS.reconfigure :exhale @15:44:22.45 SS.hold @15:44:59.34 Intake.select :close @15:44:59.50 SSV.dial :exhaust @15:45:00.80 Exhaust.select :open @15:45:00.92 SS.seek 0ml @15:45:09.78 Exhaust.select :close @15:45:09.89 SSV.dial CBV @15:45:09.92 CBV.dial SSV @15:45:10.74 Allowing 130s for 25.0ml of remaining 400.0ml @15:45:10.75 Intake.select :open @15:45:12.92 Intake @16.2psia, vacuum from 10.0 to 13.0psi @15:45:12.94 SS.reconfigure :inhaling 0.38ml/s @15:45:12.99 SS.seek 25ml @15:46:11.52 Vacuum too high -- waiting up to 40 seconds @15:46:11.54 SS.reconfigure :exhale @15:46:11.59 SS.hold @15:46:14.29 recoverPSRreagent08 @15:46:14.30 PTV.dial :puck @15:46:14.37 PBV.dial :waste1 @15:46:14.75 delay 30 seconds @15:46:26.59 Intake @16.7psia, vacuum from 10.0 to 13.0psi @15:46:26.61 SS.reconfigure :inhaling 0.31ml/s @15:46:26.66 SS.seek 25ml @15:46:39.50 Stroke completed -- waiting for pressure to equalize ... @15:46:39.51 SS.reconfigure :exhale @15:46:39.56 SS.hold @15:46:44.76 PBV.dial between PSV and waste1 @15:46:45.14 PSR.select :air @15:46:45.16 PSV.dial PSR @15:46:45.92 PS.reconfigure :fast @15:46:45.97 PS.seek 2.5ml @15:47:02.31 Intake.select :close @15:47:02.47 SSV.dial :exhaust @15:47:03.23 Exhaust.select :open @15:47:03.35 SS.seek 0ml @15:47:09.98 PSV.dial PTV @15:47:10.87 delay 5 seconds @15:47:12.39 Exhaust.select :close @15:47:12.50 SSV.dial CBV @15:47:12.53 CBV.dial SSV @15:47:13.80 Allowing 130s for 25.0ml of remaining 375.0ml @15:47:13.82 Intake.select :open @15:47:15.89 PSV.dial PSR @15:47:16.00 Intake @16.2psia, vacuum from 10.0 to 13.0psi @15:47:16.02 SS.reconfigure :inhaling 0.31ml/s @15:47:16.07 SS.seek 25ml @15:47:16.64 delay 5 seconds @15:47:21.66 PSV.dial PTV @15:47:22.44 delay 5 seconds @15:47:27.46 PSV.dial :air @15:47:28.35 PS.seek 0ml @15:47:51.85 Gate.power :camera,:ON @15:47:51.87 Mixing DA Substrates @15:47:51.88 PSR.select :air @15:47:51.89 PSV.dial PSR @15:47:53.31 Pulling 0.25ml of PSR.sub1 @15:47:53.35 PSR.select :sub1 @15:47:53.54 PS.reconfigure :default @15:47:53.66 PS.seek 0.25ml @15:47:57.27 delay 5 seconds @15:48:02.29 Pulling 0.25ml of PSR.sub2 @15:48:02.31 PSR.select :sub2 @15:48:02.43 PS.seek 0.5ml @15:48:05.86 delay 5 seconds @15:48:10.88 PSR.select :air @15:48:10.99 PS.seek 2ml @15:48:27.23 PTV.dial :puck @15:48:27.31 PSV.dial PTV @15:48:28.14 PBV.dial :waste1 @15:48:28.59 PS.seek 1.5ml @15:48:34.54 delay 3 seconds @15:48:37.56 PBV.dial between PSV and waste1 @15:48:37.95 PS.seek 0ml @15:48:42.82 Stroke completed -- waiting for pressure to equalize ... @15:48:42.84 SS.reconfigure :exhale @15:48:42.89 SS.hold @15:48:54.17 delay 10 seconds @15:49:04.18 recoverPSRreagent08 @15:49:04.20 PTV.dial :puck @15:49:04.28 PBV.dial :waste1 @15:49:04.65 delay 30 seconds @15:49:17.71 Intake.select :close @15:49:17.87 SSV.dial :exhaust @15:49:18.65 Exhaust.select :open @15:49:18.77 SS.seek 0ml @15:49:27.87 Exhaust.select :close @15:49:27.99 SSV.dial CBV @15:49:28.04 CBV.dial SSV @15:49:28.76 Allowing 130s for 25.0ml of remaining 350.0ml @15:49:28.78 Intake.select :open @15:49:30.94 Intake @16.3psia, vacuum from 10.0 to 13.0psi @15:49:30.96 SS.reconfigure :inhaling 0.31ml/s @15:49:31.01 SS.seek 25ml @15:49:34.67 PBV.dial between PSV and waste1 @15:49:35.04 PSR.select :air @15:49:35.48 PSV.dial PSR @15:49:36.44 PS.reconfigure :fast @15:49:36.49 PS.seek 2.5ml @15:50:00.82 PSV.dial PTV @15:50:01.59 delay 5 seconds @15:50:06.61 PSV.dial PSR @15:50:07.39 delay 5 seconds @15:50:12.41 PSV.dial PTV @15:50:13.18 delay 5 seconds @15:50:18.20 PSV.dial :air @15:50:18.98 PS.seek 0ml @15:50:43.01 Additional air clear @15:50:43.02 PSR.select :air @15:50:43.04 PSV.dial PSR @15:50:44.40 Pulling 2ml of PSR.air @15:50:44.41 PSR.select :air @15:50:44.43 PS.reconfigure :default @15:50:44.48 PS.seek 2ml @15:50:57.60 Stroke completed -- waiting for pressure to equalize ... @15:50:57.62 SS.reconfigure :exhale @15:50:57.66 SS.hold @15:51:05.82 delay 5 seconds @15:51:10.84 PTV.dial :puck @15:51:10.91 PSV.dial PTV @15:51:12.16 PBV.dial :waste1 @15:51:12.60 PS.seek 1.5ml @15:51:18.57 delay 3 seconds @15:51:21.59 PBV.dial between PSV and waste1 @15:51:21.96 delay 1 second @15:51:22.98 PSV.dial :waste1 @15:51:24.06 PS.seek 0ml @15:51:36.51 Intake.select :close @15:51:36.67 SSV.dial :exhaust @15:51:37.44 Exhaust.select :open @15:51:37.56 SS.seek 0ml @15:51:40.31 Processing Puck Heater at 27.9C before imaging @15:51:40.32 PH.coast @15:51:40.44 Imaging da puck in Processing Clamp while storing it to tube 4 @15:51:40.62 SC.reconfigure :tight @15:51:40.67 SC.seek 4 @15:51:44.30 SC.configure :limp @15:51:44.52 Elbow.reconfigure :tight @15:51:44.57 Elbow.seek 4 @15:51:46.78 Exhaust.select :close @15:51:46.82 Forearm.reconfigure :tight @15:51:46.93 Forearm.seek 4 + 450 counts @15:51:46.99 SSV.dial CBV @15:51:47.01 CBV.dial SSV @15:51:47.82 Allowing 130s for 25.0ml of remaining 325.0ml @15:51:47.84 Intake.select :open @15:51:47.98 SE.reconfigure :pick @15:51:48.05 SE.seek :beamBreak @15:51:49.68 SE.configure :default @15:51:49.84 Elbow.reconfigure :limp @15:51:49.89 Forearm.reconfigure :loose @15:51:49.94 Forearm.seek 4 @15:51:50.10 Intake @16.3psia, vacuum from 10.0 to 13.0psi @15:51:50.12 SS.reconfigure :inhaling 0.31ml/s @15:51:50.19 Elbow.hold @15:51:50.22 SS.seek 25ml @15:51:50.34 Elbow.reconfigure :tight @15:51:50.44 SE.seek 22.1 Pucks below Top Plate @15:51:50.96 Forearm.force -20 @15:51:51.01 Forearm.seek 4 + 316 counts @15:51:51.40 Forearm.reconfigure :tight @15:51:51.45 Forearm.seek 4 + 450 counts @15:51:51.74 SE.seek 23.1 Pucks below Top Plate @15:51:52.37 Forearm.reconfigure :loose @15:51:52.42 Forearm.seek :retract @15:51:53.34 Elbow.seek PC @15:51:55.88 Elbow.reconfigure :loose @15:51:55.98 Forearm.seek PC @15:51:56.96 Hand.close @15:51:57.72 PC.seek :open @15:52:02.50 Forearm.seek :retract @15:52:03.45 Elbow.reconfigure :tight @15:52:03.50 Elbow.seek 4 @15:52:06.62 Forearm.reconfigure :tight @15:52:06.67 Forearm.seek 4 + 450 counts @15:52:07.61 Hand.open @15:52:08.28 SE.seek 22.75 Pucks below Top Plate @15:52:08.56 SE.reconfigure :seal @15:52:08.61 SE.seek 21.79 Pucks below Top Plate @15:52:09.15 Lights, camera, ACTION! @15:52:09.19 PuckCamera: snap -bin=2,2 -auto /var/log/esp/da23dec0615h1000ml.tif @15:52:28.67 Waiting >20 seconds for da to relinquish FlushPuck @15:52:34.31 SX HX9/16 (USB:18): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 21.754 seconds /var/log/esp/da23dec0615h1000ml.tif: TIFF Upload Complete @15:52:34.34 PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da23dec0615h1000ml15s.tif @15:52:49.99 SX HX9/16 (USB:18): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15 seconds /var/log/esp/midres/da23dec0615h1000ml15s.tif: TIFF Upload Complete @15:52:50.02 PuckCamera: snap -bin=2,2 20.0 /var/log/esp/midres/da23dec0615h1000ml20s.tif @15:53:10.67 SX HX9/16 (USB:18): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 20 seconds /var/log/esp/midres/da23dec0615h1000ml20s.tif: TIFF Upload Complete @15:53:10.69 Gate.power :camera,:OFF @15:53:10.71 DAfans.email "21.754 sec auto-exposure",Subject: "da23dec0615h1000ml images!" @15:53:11.18 Elbow.seek 4 @15:53:11.40 Forearm.seek 4 + 450 counts @15:53:11.49 SE.reconfigure :gently @15:53:11.54 SE.seek 22.51 Pucks below Top Plate @15:53:12.27 Elbow.reconfigure :limp @15:53:12.33 Forearm.reconfigure :loose @15:53:12.40 Forearm.seek 4 @15:53:12.62 Elbow.hold @15:53:12.72 Elbow.reconfigure :tight @15:53:12.78 SE.reconfigure :default @15:53:12.84 SE.seek 22.69 Pucks below Top Plate @15:53:13.31 Forearm.force -20 @15:53:13.36 Forearm.seek 4 + 318 counts @15:53:13.66 Forearm.reconfigure :tight @15:53:13.72 Forearm.seek 4 + 450 counts @15:53:13.99 SE.seek :down @15:53:15.15 SE.stop @15:53:15.20 Forearm.reconfigure :loose @15:53:15.34 Forearm.seek :retract @15:53:16.24 Vacuum too high -- waiting up to 40 seconds @15:53:16.28 SS.reconfigure :exhale @15:53:17.65 SS.hold @15:53:17.94 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @15:53:18.80 Elbow.seek FlushPuck::Garage @15:53:20.80 Elbow.reconfigure :loose @15:53:21.02 Forearm.seek :garage @15:53:22.12 Hand.close @15:53:23.13 Forearm.seek :retract @15:53:24.38 Elbow.reconfigure :tight @15:53:24.50 Elbow.seek PC @15:53:26.02 Elbow.reconfigure :loose @15:53:26.44 Forearm.reconfigure :tight @15:53:26.54 Forearm.seek PC @15:53:29.07 PC.seek :closed @15:53:34.16 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.64 @15:53:34.31 Hand.open @15:53:35.13 Forearm.reconfigure :loose @15:53:35.23 Forearm.seek :retract @15:53:36.21 PSR.select :air @15:53:36.26 PSV.dial PSR @15:53:36.71 Intake @18.1psia, vacuum from 10.0 to 13.0psi @15:53:36.76 SS.reconfigure :inhaling 0.25ml/s @15:53:36.90 SS.seek 25ml @15:53:36.94 Pulling 0.5ml of PSR.flush @15:53:36.99 PSR.select :flush @15:53:37.20 PS.seek 0.5ml @15:53:40.21 Stroke completed -- waiting for pressure to equalize ... @15:53:40.26 SS.reconfigure :exhale @15:53:40.38 SS.hold @15:53:43.25 delay 5 seconds @15:53:48.32 PSR.select :air @15:53:48.48 PS.seek 2.5ml @15:53:48.86 upsync @15:53:57.85 SS.reconfigure :default @15:53:57.90 SS.hold @15:53:57.97 Intake.select :close @15:53:58.13 ShallowSampler::Clogged in WCR -- Filtering too Slowly after 700.0ml @15:53:58.14 SSV.dial :exhaust @15:53:58.91 Exhaust.select :open @15:53:59.03 SS.reconfigure :exhale @15:53:59.08 SS.seek 0ml @15:54:07.82 Exhaust.select :close @15:54:07.93 Sampled 700.0ml @15:54:07.95 Drying puck before fixing for 5:00 @15:54:07.96 CBV.dial :waste2b @15:54:08.73 delay 1:00 @15:54:09.79 delay 1 second @15:54:10.81 PRV.dial :waste2,avoiding: :ab2 @15:54:12.68 PTV.dial :PRVmixing @15:54:14.00 PSV.dial :mixloop @15:54:15.06 PS.seek :empty @15:54:41.41 PSR.select :air @15:54:41.43 PSV.dial PSR @15:54:42.54 Pulling 0.5ml of PSR.flush @15:54:42.55 PSR.select :flush @15:54:42.67 PS.seek 0.5ml @15:54:48.60 delay 5 seconds @15:54:53.62 PSR.select :air @15:54:53.74 PS.seek 2.5ml @15:55:08.75 CSV.dial :air @15:55:09.21 CS.reconfigure :fast @15:55:09.26 CS.seek 10ml @15:55:14.97 delay 1 second @15:55:15.99 PBV.dial :waste1 @15:55:16.36 PTV.dial :mixing @15:55:17.11 PSV.dial :mixloop @15:55:18.63 PS.seek :empty @15:55:33.48 Pumping wcr puck from tube 5 up to 35psi... @15:55:33.50 CBV.dial SSV @15:55:34.79 CBV.dial between CSV and waste2b @15:55:35.76 CSV.dial between air and CTV @15:55:36.08 CS.seek 7ml @15:55:43.71 CTV.dial CSV,avoiding: :air @15:55:43.76 CSV.dial CTV @15:55:44.53 CS.reconfigure :pressurize @15:55:44.59 CS.seek 0ml @15:55:44.97 PSV.dial PSR @15:55:46.03 PSR.select :flush @15:55:46.16 PS.seek 1ml @15:55:57.19 PSR.select :air @15:55:57.30 PS.seek 2.5ml @15:56:02.45 CBV.dial :waste2b @15:56:02.78 CS.reconfigure :default @15:56:02.83 CS.seek 0ml @15:56:04.04 delay 1:00 @15:56:13.41 PSV.dial PTV @15:56:14.17 PTV.dial :puck @15:56:14.92 PBV.dial :waste1 @15:56:15.01 PS.seek 0ml @15:56:41.41 PSV.dial PSR @15:56:42.17 PSR.select :flush @15:56:42.30 PS.seek 0.5ml @15:56:48.28 PSR.select :air @15:56:48.40 PS.seek 2.5ml @15:57:04.07 CTV.dial :air,avoiding: :intake @15:57:05.41 Fix #1 of 2 (with RNAlater) for 2:30 @15:57:05.42 CSV.dial CSR @15:57:05.87 Pulling 2ml of CSR.RNAlater @15:57:05.88 CSR.select :RNAlater @15:57:06.00 CS.seek 2ml @15:57:09.73 PSV.dial PTV @15:57:10.49 PBV.dial PSV @15:57:11.07 PS.seek 0ml @15:57:11.98 delay 5 seconds @15:57:17.00 CSR.select :air @15:57:17.11 CS.reconfigure :fast @15:57:17.16 CS.seek 10ml @15:57:37.20 Pumping wcr puck from tube 5 up to 35psi... @15:57:37.22 CBV.dial SSV @15:57:37.41 PSV.dial PBV @15:57:37.88 PS.seek 2.5ml @15:57:38.49 CBV.dial between CSV and waste2b @15:57:39.48 CSV.dial between air and CTV @15:57:40.10 CS.seek 6.946ml @15:57:48.02 CTV.dial CSV,avoiding: :air @15:57:48.04 CSV.dial CTV @15:57:49.42 CS.reconfigure :pressurize @15:57:49.48 CS.seek 0ml @15:58:02.34 delay 2:30 @15:58:04.25 PSV.dial :waste1 @15:58:05.01 PS.seek 0ml @15:58:31.34 PSV.dial PSR @15:58:31.81 PSR.select :air @15:58:31.84 PS.seek 2.5ml @15:58:58.22 PSV.dial PTV @15:58:58.97 PBV.dial PSV @15:58:59.16 PS.seek 0ml @15:59:25.50 PSV.dial PBV @15:59:25.97 PS.seek 2.5ml @15:59:52.25 PSV.dial :waste1 @15:59:53.04 PS.seek 0ml @16:00:19.34 PSV.dial PSR @16:00:19.81 PSR.select :flush @16:00:19.94 PS.seek 1ml @16:00:31.03 PSR.select :air @16:00:31.32 PS.seek 2.5ml @16:00:32.36 CBV.dial :waste2b @16:00:32.72 CS.reconfigure :default @16:00:32.77 CS.seek 0ml @16:00:38.88 delay 1:00 @16:00:47.69 PSV.dial PTV @16:00:48.55 PTV.dial :puck @16:00:48.62 PBV.dial :waste1 @16:00:49.18 PS.seek 0ml @16:01:15.75 PSV.dial PSR @16:01:16.50 PSR.select :flush @16:01:17.07 PS.seek 0.5ml @16:01:23.09 PSR.select :air @16:01:23.21 PS.seek 2.5ml @16:01:38.91 CTV.dial :air,avoiding: :intake @16:01:40.23 Fix #2 of 2 (with RNAlater) for 2:30 @16:01:40.25 CSV.dial CSR @16:01:40.68 Pulling 2ml of CSR.RNAlater @16:01:40.69 CSR.select :RNAlater @16:01:40.81 CS.seek 2ml @16:01:44.41 PSV.dial PTV @16:01:45.17 PBV.dial PSV @16:01:45.74 PS.seek 0ml @16:01:46.95 delay 5 seconds @16:01:51.97 CSR.select :air @16:01:52.08 CS.reconfigure :fast @16:01:52.13 CS.seek 10ml @16:02:11.73 Pumping wcr puck from tube 5 up to 35psi... @16:02:11.75 CBV.dial SSV @16:02:12.09 PSV.dial PBV @16:02:12.57 PS.seek 2.5ml @16:02:13.00 CBV.dial between CSV and waste2b @16:02:13.97 CSV.dial between air and CTV @16:02:14.58 CS.seek 6.931ml @16:02:22.39 CTV.dial CSV,avoiding: :air @16:02:22.41 CSV.dial CTV @16:02:23.80 CS.reconfigure :pressurize @16:02:23.85 CS.seek 0ml @16:02:37.34 delay 2:30 @16:02:38.90 PSV.dial :waste1 @16:02:39.66 PS.seek 0ml @16:03:06.00 PSV.dial PSR @16:03:06.47 PSR.select :air @16:03:06.49 PS.seek 2.5ml @16:03:32.90 PSV.dial PTV @16:03:33.65 PBV.dial PSV @16:03:33.83 PS.seek 0ml @16:04:00.15 PSV.dial PBV @16:04:00.63 PS.seek 2.5ml @16:04:26.99 PSV.dial :waste1 @16:04:27.75 PS.seek 0ml @16:04:54.09 Garaging FlushPuck in Processing Clamp @16:04:54.16 Elbow.reconfigure :tight @16:04:54.21 Elbow.seek PC @16:04:54.33 Elbow.reconfigure :loose @16:04:54.44 Forearm.seek PC @16:04:55.42 Hand.close @16:04:56.08 PC.seek :open @16:05:00.84 Forearm.seek :retract @16:05:01.77 Elbow.reconfigure :tight @16:05:01.82 Elbow.seek :garage @16:05:03.19 Elbow.reconfigure :loose @16:05:03.29 Forearm.reconfigure :tight @16:05:03.34 Forearm.seek :garage @16:05:04.38 Hand.open @16:05:05.02 Forearm.reconfigure :loose @16:05:05.07 Forearm.seek :retract @16:05:07.36 CBV.dial :waste2b @16:05:07.71 CS.reconfigure :default @16:05:07.82 CS.seek 0ml @16:05:13.22 delay 1:00 @16:06:13.25 CTV.dial :air,avoiding: :intake @16:06:14.63 Storing wcr puck in Collection Clamp to tube 4 @16:06:15.04 Elbow.reconfigure :tight @16:06:15.10 Elbow.seek 4 @16:06:16.98 Forearm.reconfigure :tight @16:06:17.03 Forearm.seek 4 + 450 counts @16:06:18.05 SE.reconfigure :pick @16:06:18.11 SE.seek :beamBreak @16:06:19.73 SE.configure :default @16:06:20.36 Elbow.reconfigure :limp @16:06:20.41 Forearm.reconfigure :loose @16:06:20.46 Forearm.seek 4 @16:06:20.74 Elbow.hold @16:06:20.81 Elbow.reconfigure :tight @16:06:20.91 SE.seek 23.1 Pucks below Top Plate @16:06:21.44 Forearm.force -20 @16:06:21.49 Forearm.seek 4 + 305 counts @16:06:21.79 Forearm.reconfigure :tight @16:06:21.84 Forearm.seek 4 + 450 counts @16:06:22.05 SE.seek 24.1 Pucks below Top Plate @16:06:22.68 Forearm.reconfigure :loose @16:06:22.74 Forearm.seek :retract @16:06:23.66 Elbow.seek CC @16:06:25.22 Elbow.reconfigure :loose @16:06:25.32 Forearm.seek CC @16:06:26.31 Hand.close @16:06:26.96 CC.seek :open @16:06:31.74 Forearm.seek :retract @16:06:32.75 Elbow.reconfigure :tight @16:06:32.81 Elbow.seek 4 @16:06:34.57 Elbow.reconfigure :limp @16:06:34.62 Forearm.seek 4 @16:06:35.61 Elbow.hold @16:06:35.68 Elbow.reconfigure :tight @16:06:35.73 Hand.open @16:06:36.90 Forearm.force -20 @16:06:36.95 Forearm.seek 4 + 315 counts @16:06:37.35 Forearm.reconfigure :tight @16:06:37.40 Forearm.seek 4 + 450 counts @16:06:37.65 SE.seek :down @16:06:38.79 SE.stop @16:06:38.82 Forearm.reconfigure :loose @16:06:38.91 Forearm.seek :retract @16:06:39.90 Starting finalPurge @16:06:39.91 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @16:06:40.10 Elbow.seek FlushPuck::Garage @16:06:41.90 Elbow.reconfigure :loose @16:06:42.00 Forearm.seek :garage @16:06:42.95 Hand.close @16:06:43.70 Forearm.seek :retract @16:06:44.66 Elbow.reconfigure :tight @16:06:44.71 Elbow.seek CC @16:06:47.65 Elbow.reconfigure :loose @16:06:47.75 Forearm.reconfigure :tight @16:06:47.80 Forearm.seek CC @16:06:49.06 CC.seek :closed @16:06:54.08 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.33 @16:06:54.20 Hand.open @16:06:54.90 Forearm.reconfigure :loose @16:06:54.94 Forearm.seek :retract @16:06:55.93 Flushing the Collection Valve::Manifold @16:06:56.05 CSV.dial CSR @16:06:56.49 Pulling 5ml of CSR.flush @16:06:56.50 CSR.select :flush @16:06:56.62 CS.seek 5ml @16:07:10.41 delay 5 seconds @16:07:15.43 CSR.select :air @16:07:15.60 CS.reconfigure :fast @16:07:15.65 CS.seek 7.5ml @16:07:22.38 CSV.dial :waste @16:07:23.68 CS.reconfigure :default @16:07:23.73 CS.seek 0ml @16:07:43.75 Flushing lower manifold's waste1 port @16:07:43.88 CSV.dial :flush @16:07:44.32 CS.reconfigure :fast @16:07:44.37 CS.seek 5ml @16:07:57.00 delay 5 seconds @16:08:02.02 CSV.dial :air @16:08:02.55 CS.seek 7ml @16:08:08.04 CSV.dial CTV @16:08:08.08
CTV.dial CSV @16:08:09.44 CBV.dial :waste1 @16:08:10.21 CS.seek 0ml @16:08:26.72 Flushing lower manifold's waste2 port @16:08:26.85 CSV.dial :flush @16:08:27.59 CS.seek 5ml @16:08:40.13 delay 5 seconds @16:08:45.15 CSV.dial :air @16:08:45.67 CS.seek 6.999ml @16:08:51.32 CSV.dial CTV @16:08:51.36
CTV.dial CSV @16:08:51.78 CBV.dial :waste2 @16:08:52.84 CS.seek 0ml @16:09:09.32 Flushing lower manifold's waste2b port @16:09:09.44 CSV.dial :flush @16:09:10.18 CS.seek 5ml @16:09:22.97 delay 5 seconds @16:09:27.99 CSV.dial :air @16:09:28.52 CS.seek 7ml @16:09:34.10 CSV.dial CTV @16:09:34.60
CTV.dial CSV @16:09:35.02 CBV.dial :waste2b @16:09:36.07 CS.seek 0ml @16:09:52.57 Disinfecting external Intake with kill @16:09:52.69 CSV.dial CSR @16:09:53.25 Pulling 8ml of CSR.kill @16:09:53.26 CSR.select :kill @16:09:53.38 CS.seek 8ml @16:10:12.94 delay 5 seconds @16:10:17.96 CSR.select :air @16:10:18.08 CS.seek 10ml @16:10:23.57 CSV.dial CBV @16:10:23.59
CBV.dial CSV @16:10:24.69 CTV.dial :intake @16:10:25.47 Intake.select :open @16:10:25.58 CS.seek 2ml @16:10:44.28 Intake.select :close @16:10:44.39 CTV.dial :air @16:10:45.14 CS.reconfigure :default @16:10:45.19 CS.seek 0ml @16:10:51.19 CSV.dial CSR @16:10:52.20 Pulling 5ml of CSR.flush @16:10:52.21 CSR.select :flush @16:10:52.33 CS.seek 5ml @16:11:06.25 delay 5 seconds @16:11:11.27 CSR.select :air @16:11:11.39 CS.seek 10ml @16:11:25.00 CSV.dial CBV @16:11:26.02 CS.seek 0ml @16:11:52.53 Garaging FlushPuck in Collection Clamp @16:11:52.60 Elbow.reconfigure :tight @16:11:52.65 Elbow.seek CC @16:11:52.98 Elbow.reconfigure :loose @16:11:53.08 Forearm.seek CC @16:11:54.14 Hand.close @16:11:54.70 CC.seek :open @16:11:59.36 Forearm.seek :retract @16:12:00.39 Elbow.reconfigure :tight @16:12:00.44 Elbow.seek :garage @16:12:03.27 Elbow.reconfigure :loose @16:12:03.37 Forearm.reconfigure :tight @16:12:03.42 Forearm.seek :garage @16:12:04.36 Hand.open @16:12:05.01 Forearm.reconfigure :loose @16:12:05.06 Forearm.seek :retract @16:12:06.14 Completed [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] begun 13:26:41 @16:12:06.20 Asleep after completing DA_WCR_120623 assays @16:12:06.22 GoodNews.email "{1 => 33,\n 2 => 33,\n 3 => 31,\n 4 => 25,\n 5 => 8,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@16:06:02, 30% flow, 17.2C, 31% humidity, 15.1psia, 13.705V, 0.429A, 162.872Ah, 5.88W\nSampled 700.0ml",Subject: "Asleep after completing DA_WCR_120623 assays" @16:12:10.81 upsync + ESPwhich DA_WCR_120623b.rb + fn=DA_WCR_120623b.rb + IFS=: + set /home/esp/esp2/mission . + fullPath=/home/esp/esp2/mission/DA_WCR_120623b.rb + [ -f /home/esp/esp2/mission/DA_WCR_120623b.rb ] + realpath /home/esp/esp2/mission/DA_WCR_120623b.rb + return 0 + scriptPath=/home/esp/esp2local/mission/DA_WCR_120623b.rb + exec ruby /home/esp/esp2/lib/esp.rb /home/esp/esp2local/mission/DA_WCR_120623b.rb @21:31:33.77UTC06-Dec-23
ESP2 v3.42 I2C Gateway v3.20 for core bus @21:31:36.44 Configuration for short puck jake 1/11/21 by brent@mbari.org /home/esp/esp2/type/shallow/jake/configure.rb @21:31:40.14 Polling Can every 10:00 @21:31:40.22 Cleanly exited at 20:55:56UTC06-Dec-23 @21:31:40.27 Loading /home/esp/esp2local/mission/DA_WCR_120623b.rb mission @21:31:40.33 Preparing to sleep until + 00:00:05 @21:31:40.34 GoodNews.email "Starting DA_WCR_120623b w/pucks in tube 5\n{1 => 33,\n 2 => 33,\n 3 => 31,\n 4 => 25,\n 5 => 8,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@21:31:40, 31% flow, 16.5C, 32% humidity, 15.1psia, 13.734V, 0.302A, 164.329Ah, 4.15W",Subject: "DA_WCR_120623b begun" @21:31:40.40
Asleep til + 00:00:05 @21:31:40.42 Continuing at 21:31 @21:31:40.66 Gate.power :core,:OFF @21:31:40.68 Gate.power :raw,:OFF @21:31:40.69 Shutdown @21:31:43.91 GoodNews.email "Then, in tube 5: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_120623b.rb:14\n{1 => 33,\n 2 => 33,\n 3 => 31,\n 4 => 25,\n 5 => 8,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@21:31:40, 31% flow, 16.5C, 32% humidity, 15.1psia, 13.734V, 0.296A, 164.329Ah, 4.07W",Subject: "Asleep until 21:31" @21:31:45.23 upsync @21:31:45.33
Powering core bus @21:31:48.33 upsync @21:31:51.01
Gate.power :raw,:ON @21:31:51.67 CS.configure :default @21:31:51.72 PS.configure :default @21:31:51.76 SS.configure :default @21:31:51.81 CC.configure :default @21:31:51.86 PC.configure :default @21:31:51.93 Forearm.configure :loose @21:31:51.97 Elbow.configure :loose @21:31:52.04 SC.configure :loose @21:31:52.09 SE.configure :default @21:31:52.14 CH.configure :default @21:31:52.17 PH.configure :default @21:31:52.21 All dwarf controllers running firmware version 3.85 @21:31:52.32 Valve.reset @21:31:52.55 Intake.select :close @21:31:52.60 Exhaust.select :close @21:31:52.64 CTV.dial :air @21:31:52.73 PSV.dial :air @21:31:52.87 CSV.dial :waste1 @21:31:52.89
Forearm.home.seek :retract @21:31:53.35 SSV.dial :spit,avoiding: :exhaust @21:31:53.63 Hand.close @21:31:53.67
Elbow.reconfigure :loose @21:31:53.76 Elbow.jog 1500 @21:31:53.84 PS.home.seek 0ml @21:31:54.22 CS.reconfigure :fast @21:31:54.27 SS.home.seek 0ml @21:31:54.35 CS.home.seek 0ml @21:31:54.42
Elbow.reconfigure :loose @21:31:54.48 Elbow.home.seek :clear @21:31:57.82 SE.home.seek :down @21:31:58.47 SC.reconfigure :tight @21:31:58.53 SC.home.seek :initial @21:32:02.14 PS.stop @21:32:17.38
SC.configure :limp @21:32:17.43 Forearm.seek :retract @21:32:17.50 Elbow.seek :hammer @21:32:18.51 Forearm.reconfigure :tight @21:32:18.57 Forearm.seek retracted @21:32:18.98 PC.home.seek :open @21:32:19.01
CC.home.seek :open @21:32:22.78 Phase [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] @21:32:22.79 Notice.email "At 21:32:22 began in tube 5: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_120623b.rb:14\n{1 => 33,\n 2 => 33,\n 3 => 31,\n 4 => 25,\n 5 => 8,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@21:32:22, 31% flow, 16.5C, 32% humidity, 15.1psia, 13.681V, 0.272A, 164.334Ah, 3.72W",Subject: "Started DA_WCR_120623b phase initialPurge; da [1000, 1000] #da and wsr are run simultanelously" @21:32:22.87
Starting initialPurge @21:32:22.88 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @21:32:22.96 Hand.open @21:32:23.65 Elbow.reconfigure :tight @21:32:23.70 Elbow.seek FlushPuck::Garage @21:32:25.50 Elbow.reconfigure :loose @21:32:25.63 Forearm.reconfigure :loose @21:32:25.68 Forearm.seek :garage @21:32:26.64 Hand.close @21:32:27.14 upsync @21:32:27.40
Forearm.seek :retract @21:32:28.36 Elbow.reconfigure :tight @21:32:28.41 Elbow.seek CC @21:32:31.36 Elbow.reconfigure :loose @21:32:31.47 Forearm.reconfigure :tight @21:32:31.52 Forearm.seek CC @21:32:32.76 CC.seek :closed @21:32:37.29 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.46 @21:32:37.36 Hand.open @21:32:38.09 Forearm.reconfigure :loose @21:32:38.13 Forearm.seek :retract @21:32:39.09 Flushing the Collection Valve::Manifold @21:32:39.21 CSV.dial CSR @21:32:40.51 Pulling 5ml of CSR.flush @21:32:40.52 CSR.select :flush @21:32:40.64 CS.reconfigure :default @21:32:40.69 CS.seek 5ml @21:32:54.72 delay 5 seconds @21:32:59.74 CSR.select :air @21:32:59.91 CS.reconfigure :fast @21:32:59.96 CS.seek 7.5ml @21:33:06.69 CSV.dial :waste @21:33:07.98 CS.reconfigure :default @21:33:08.03 CS.seek 0ml @21:33:28.10 Purging external Intake @21:33:28.22 CSV.dial :flush @21:33:28.67 CS.reconfigure :fast @21:33:28.71 CS.seek 10ml @21:33:53.13 delay 5 seconds @21:33:58.15 CSV.dial CBV @21:33:58.18
CBV.dial CSV @21:33:58.94 CTV.dial :intake @21:33:59.72 Intake.select :open @21:33:59.84 CS.seek 0ml @21:34:23.35 Intake.select :close @21:34:23.45 CTV.dial :air @21:34:24.23 Flushing upper manifold @21:34:24.35 CSV.dial :flush @21:34:25.09 CS.seek 5ml @21:34:37.72 delay 5 seconds @21:34:42.74 CSV.dial :air @21:34:43.27 CS.seek 7.001ml @21:34:48.85 CSV.dial CBV @21:34:48.87
CBV.dial CSV @21:34:49.93 CTV.dial :air @21:34:50.01 CS.seek 0ml @21:35:06.54 delay 7 seconds @21:35:13.56 CS.seek 3ml @21:35:21.75 CSV.dial :waste @21:35:22.21 CS.seek 0ml @21:35:29.99 CSV.dial :flush @21:35:30.56 CS.seek 3ml @21:35:38.53 delay 5 seconds @21:35:43.55 CSV.dial :air @21:35:44.08 CS.seek 5ml @21:35:49.56 CSV.dial :air @21:35:49.76 CS.seek 0ml @21:36:02.03 delay 7 seconds @21:36:09.06 CS.seek 3ml @21:36:17.03 CSV.dial :waste @21:36:17.76 CS.seek 0ml @21:36:25.44 Garaging FlushPuck in Collection Clamp @21:36:25.51 Elbow.reconfigure :tight @21:36:25.56 Elbow.seek CC @21:36:25.89 Elbow.reconfigure :loose @21:36:26.00 Forearm.seek CC @21:36:26.94 Hand.close @21:36:27.53 CC.seek :open @21:36:32.60 Forearm.seek :retract @21:36:33.59 Elbow.reconfigure :tight @21:36:33.63 Elbow.seek :garage @21:36:36.59 Elbow.reconfigure :loose @21:36:36.70 Forearm.reconfigure :tight @21:36:36.74 Forearm.seek :garage @21:36:37.70 Hand.open @21:36:38.33 Forearm.reconfigure :loose @21:36:38.38 Forearm.seek :retract @21:36:39.37 da sampling at most 1000ml, wcr at most 1000ml @21:36:39.40 Loading sh1 puck from tube 5 into Collection Clamp @21:36:39.42 DAfans.email "Started da in tube 5 at 21:36:39UTC06-Dec-23\n{1 => 33,\n 2 => 33,\n 3 => 31,\n 4 => 25,\n 5 => 8,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@21:32:22, 31% flow, 16.5C, 32% humidity, 15.1psia, 13.681V, 0.272A, 164.334Ah, 3.72W",Subject: "da sampling at most 1000ml, wcr at most 1000ml" @21:36:39.69
SC.reconfigure :tight @21:36:39.76 SC.seek 5 @21:36:43.32 SC.configure :limp @21:36:43.57 Elbow.reconfigure :tight @21:36:43.63 Elbow.seek 5 @21:36:43.76 upsync @21:36:45.59
Forearm.reconfigure :tight @21:36:45.64 Forearm.seek 5 + 450 counts @21:36:46.66 SE.reconfigure :pick @21:36:46.73 SE.seek :beamBreak @21:36:49.38 SE.configure :default @21:36:49.55 Elbow.seek 5 @21:36:49.85 Forearm.seek 5 + 300 counts @21:36:50.07 Forearm.reconfigure :loose @21:36:50.11 Hand.close @21:36:50.79 SE.seek :down @21:36:51.36
Forearm.seek :retract @21:36:52.38 Elbow.seek CC @21:36:53.08 SE.stop @21:36:54.03
Elbow.reconfigure :loose @21:36:54.13 Forearm.reconfigure :tight @21:36:54.18 Forearm.seek CC @21:36:55.40 CC.seek :closed @21:36:59.94 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.28 @21:36:59.96 Hand.open @21:37:00.59 Forearm.reconfigure :loose @21:37:00.64 Forearm.seek :retract @21:37:01.59 collectSample 1000ml w/bubble point of 24psi @21:37:01.60 Priming 7ml @21:37:01.61 Intake.select :close @21:37:01.62 Exhaust.select :close @21:37:01.63 SS.hold @21:37:01.84 Calibrating pressure and temperature sensors @21:37:01.87 CTV.dial :air @21:37:01.88 SSV.dial :spit,avoiding: :exhaust @21:37:01.96 SS.reconfigure :fast @21:37:02.01 SS.seek 5ml @21:37:04.84 SSV.dial CBV @21:37:04.88
CBV.dial SSV @21:37:06.20 delay 3.8 seconds @21:37:10.13 Sensor limits: Intake max 101.5psia; Outlet max 94.2psia @21:37:10.14 SSV.dial :spit @21:37:11.48 SS.seek 0ml @21:37:14.28 SSV.dial CBV @21:37:15.59 CTV.dial :intake @21:37:16.38 SSV.dial CBV @21:37:16.40
CBV.dial SSV @21:37:16.55 Allowing 40s for 7.0ml of remaining 7.0ml @21:37:16.57 Intake.select :open @21:37:18.73 Intake @14.3psia, vacuum from 8.8 to 11.8psi @21:37:18.75 SS.reconfigure :inhaling 2.20ml/s @21:37:18.80 SS.seek 7ml @21:37:25.46 Stroke completed -- waiting for pressure to equalize ... @21:37:25.47 SS.reconfigure :exhale @21:37:25.52 SS.hold @21:37:34.25 Intake.select :close @21:37:34.41 SSV.dial :exhaust @21:37:35.15 Exhaust.select :open @21:37:35.27 SS.seek 0ml @21:37:39.47 Exhaust.select :close @21:37:39.58 Sampling 1000.0ml @21:37:39.59 Intake.select :close @21:37:39.60 Exhaust.select :close @21:37:39.61 SS.reconfigure :default @21:37:39.65 SS.hold @21:37:39.86 CTV.dial :intake @21:37:40.04 SSV.dial CBV @21:37:40.07
CBV.dial SSV @21:37:40.83 Allowing 130s for 25.0ml of remaining 1000.0ml @21:37:40.84 Intake.select :open @21:37:43.00 Intake @15.2psia, vacuum from 9.7 to 12.7psi @21:37:43.01 SS.reconfigure :inhaling 2.20ml/s @21:37:43.06 SS.seek 25ml @21:37:50.76 Vacuum too high -- waiting up to 40 seconds @21:37:50.77 SS.reconfigure :exhale @21:37:50.82 SS.hold @21:37:56.48 Intake @18.7psia, vacuum from 10.0 to 13.0psi @21:37:56.49 SS.reconfigure :inhaling 1.76ml/s @21:37:56.54 SS.seek 25ml @21:37:59.10 Vacuum too high -- waiting up to 40 seconds @21:37:59.11 SS.reconfigure :exhale @21:37:59.16 SS.hold @21:38:00.21 Intake @15.6psia, vacuum from 10.0 to 13.0psi @21:38:00.22 SS.reconfigure :inhaling 1.41ml/s @21:38:00.27 SS.seek 25ml @21:38:09.49 Stroke completed -- waiting for pressure to equalize ... @21:38:09.50 SS.reconfigure :exhale @21:38:09.55 SS.hold @21:38:40.26 Intake.select :close @21:38:40.41 SSV.dial :exhaust @21:38:41.17 Exhaust.select :open @21:38:41.29 SS.seek 0ml @21:38:50.25 Exhaust.select :close @21:38:50.35 SSV.dial CBV @21:38:50.37
CBV.dial SSV @21:38:51.17 Allowing 130s for 25.0ml of remaining 975.0ml @21:38:51.18 Intake.select :open @21:38:53.35 Intake @15.8psia, vacuum from 10.0 to 13.0psi @21:38:53.36 SS.reconfigure :inhaling 1.41ml/s @21:38:53.41 SS.seek 25ml @21:39:11.35 Vacuum too high -- waiting up to 40 seconds @21:39:11.36 SS.reconfigure :exhale @21:39:11.41 SS.hold @21:39:19.05 Intake @19.5psia, vacuum from 10.0 to 13.0psi @21:39:19.06 SS.reconfigure :inhaling 1.13ml/s @21:39:19.11 SS.seek 25ml @21:39:22.16 Vacuum too high -- waiting up to 40 seconds @21:39:22.17 SS.reconfigure :exhale @21:39:22.23 SS.hold @21:39:23.93 Intake @19.1psia, vacuum from 10.0 to 13.0psi @21:39:23.94 SS.reconfigure :inhaling 0.91ml/s @21:39:23.99 SS.seek 25ml @21:39:24.79 Stroke completed -- waiting for pressure to equalize ... @21:39:24.80 SS.reconfigure :exhale @21:39:24.85 SS.hold @21:39:53.77 Intake.select :close @21:39:53.92 SSV.dial :exhaust @21:39:54.69 Exhaust.select :open @21:39:54.80 SS.seek 0ml @21:40:03.82 Exhaust.select :close @21:40:03.93 SSV.dial CBV @21:40:03.96
CBV.dial SSV @21:40:04.73 Allowing 130s for 25.0ml of remaining 950.0ml @21:40:04.74 Intake.select :open @21:40:06.91 Intake @15.7psia, vacuum from 10.0 to 13.0psi @21:40:06.92 SS.reconfigure :inhaling 0.91ml/s @21:40:06.97 SS.seek 25ml @21:40:36.59 Stroke completed -- waiting for pressure to equalize ... @21:40:36.60 SS.reconfigure :exhale @21:40:36.64 SS.hold @21:41:05.70 Intake.select :close @21:41:05.85 SSV.dial :exhaust @21:41:06.62 Exhaust.select :open @21:41:06.73 SS.seek 0ml @21:41:15.51 Exhaust.select :close @21:41:15.61 SSV.dial CBV @21:41:15.65
CBV.dial SSV @21:41:16.40 Allowing 130s for 25.0ml of remaining 925.0ml @21:41:16.41 Intake.select :open @21:41:18.58 Intake @15.8psia, vacuum from 10.0 to 13.0psi @21:41:18.59 SS.reconfigure :inhaling 0.91ml/s @21:41:18.64 SS.seek 25ml @21:41:48.76 Stroke completed -- waiting for pressure to equalize ... @21:41:48.77 SS.reconfigure :exhale @21:41:49.24 SS.hold @21:42:15.88 Intake.select :close @21:42:16.03 SSV.dial :exhaust @21:42:16.80 Exhaust.select :open @21:42:16.91 SS.seek 0ml @21:42:25.58 Exhaust.select :close @21:42:25.69 SSV.dial CBV @21:42:25.73
CBV.dial SSV @21:42:26.48 Allowing 130s for 25.0ml of remaining 900.0ml @21:42:26.49 Intake.select :open @21:42:28.66 Intake @15.8psia, vacuum from 10.0 to 13.0psi @21:42:28.67 SS.reconfigure :inhaling 0.91ml/s @21:42:28.72 SS.seek 25ml @21:42:58.57 Stroke completed -- waiting for pressure to equalize ... @21:42:58.58 SS.reconfigure :exhale @21:42:58.62 SS.hold @21:43:21.59 Intake.select :close @21:43:21.74 SSV.dial :exhaust @21:43:22.51 Exhaust.select :open @21:43:22.62 SS.seek 0ml @21:43:31.33 Exhaust.select :close @21:43:31.44 SSV.dial CBV @21:43:31.47
CBV.dial SSV @21:43:32.24 Allowing 130s for 25.0ml of remaining 875.0ml @21:43:32.25 Intake.select :open @21:43:34.42 Intake @15.8psia, vacuum from 10.0 to 13.0psi @21:43:34.43 SS.reconfigure :inhaling 0.91ml/s @21:43:34.48 SS.seek 25ml @21:44:04.49 Stroke completed -- waiting for pressure to equalize ... @21:44:04.50 SS.reconfigure :exhale @21:44:04.54 SS.hold @21:44:27.32 Intake.select :close @21:44:27.48 SSV.dial :exhaust @21:44:28.24 Exhaust.select :open @21:44:28.36 SS.seek 0ml @21:44:37.10 Exhaust.select :close @21:44:37.21 SSV.dial CBV @21:44:37.23
CBV.dial SSV @21:44:38.02 Allowing 130s for 25.0ml of remaining 850.0ml @21:44:38.03 Intake.select :open @21:44:40.20 Intake @15.8psia, vacuum from 10.0 to 13.0psi @21:44:40.22 SS.reconfigure :inhaling 0.91ml/s @21:44:40.27 SS.seek 25ml @21:45:09.96 Stroke completed -- waiting for pressure to equalize ... @21:45:09.97 SS.reconfigure :exhale @21:45:10.01 SS.hold @21:45:28.56 Intake.select :close @21:45:28.71 SSV.dial :exhaust @21:45:29.47 Exhaust.select :open @21:45:29.59 SS.seek 0ml @21:45:38.30 Exhaust.select :close @21:45:38.41 SSV.dial CBV @21:45:38.43
CBV.dial SSV @21:45:39.21 Allowing 130s for 25.0ml of remaining 825.0ml @21:45:39.22 Intake.select :open @21:45:41.46 Intake @15.8psia, vacuum from 10.0 to 13.0psi @21:45:41.48 SS.reconfigure :inhaling 0.91ml/s @21:45:41.52 SS.seek 25ml @21:46:11.39 Stroke completed -- waiting for pressure to equalize ... @21:46:11.40 SS.reconfigure :exhale @21:46:11.45 SS.hold @21:46:29.80 Intake.select :close @21:46:29.96 SSV.dial :exhaust @21:46:30.72 Exhaust.select :open @21:46:30.84 SS.seek 0ml @21:46:39.55 Exhaust.select :close @21:46:39.66 SSV.dial CBV @21:46:39.68
CBV.dial SSV @21:46:40.47 Allowing 130s for 25.0ml of remaining 800.0ml @21:46:40.48 Intake.select :open @21:46:42.65 Intake @15.8psia, vacuum from 10.0 to 13.0psi @21:46:42.67 SS.reconfigure :inhaling 0.91ml/s @21:46:42.72 SS.seek 25ml @21:47:12.48 Stroke completed -- waiting for pressure to equalize ... @21:47:12.50 SS.reconfigure :exhale @21:47:12.54 SS.hold @21:47:33.02 Intake.select :close @21:47:33.18 SSV.dial :exhaust @21:47:33.94 Exhaust.select :open @21:47:34.05 SS.seek 0ml @21:47:42.97 Exhaust.select :close @21:47:43.08 SSV.dial CBV @21:47:43.10
CBV.dial SSV @21:47:43.89 Allowing 130s for 25.0ml of remaining 775.0ml @21:47:43.90 Intake.select :open @21:47:46.07 Intake @15.7psia, vacuum from 10.0 to 13.0psi @21:47:46.08 SS.reconfigure :inhaling 0.91ml/s @21:47:46.14 SS.seek 25ml @21:48:15.67 Stroke completed -- waiting for pressure to equalize ... @21:48:15.68 SS.reconfigure :exhale @21:48:15.73 SS.hold @21:48:34.07 Intake.select :close @21:48:34.23 SSV.dial :exhaust @21:48:35.00 Exhaust.select :open @21:48:35.12 SS.seek 0ml @21:48:43.83 Exhaust.select :close @21:48:43.94 SSV.dial CBV @21:48:43.96
CBV.dial SSV @21:48:44.75 Allowing 130s for 25.0ml of remaining 750.0ml @21:48:44.76 Intake.select :open @21:48:46.93 Intake @15.8psia, vacuum from 10.0 to 13.0psi @21:48:46.94 SS.reconfigure :inhaling 0.91ml/s @21:48:46.99 SS.seek 25ml @21:49:16.76 Stroke completed -- waiting for pressure to equalize ... @21:49:16.77 SS.reconfigure :exhale @21:49:16.82 SS.hold @21:49:33.13 Intake.select :close @21:49:33.28 SSV.dial :exhaust @21:49:34.05 Exhaust.select :open @21:49:34.16 SS.seek 0ml @21:49:43.48 Exhaust.select :close @21:49:43.59 SSV.dial CBV @21:49:43.64
CBV.dial SSV @21:49:44.37 Allowing 130s for 25.0ml of remaining 725.0ml @21:49:44.39 Intake.select :open @21:49:46.56 Intake @15.8psia, vacuum from 10.0 to 13.0psi @21:49:46.57 SS.reconfigure :inhaling 0.91ml/s @21:49:46.62 SS.seek 25ml @21:50:16.06 Stroke completed -- waiting for pressure to equalize ... @21:50:16.07 SS.reconfigure :exhale @21:50:16.12 SS.hold @21:50:32.33 Intake.select :close @21:50:32.49 SSV.dial :exhaust @21:50:33.25 Exhaust.select :open @21:50:33.36 SS.seek 0ml @21:50:42.08 Exhaust.select :close @21:50:42.19 SSV.dial CBV @21:50:42.23
CBV.dial SSV @21:50:42.97 Allowing 130s for 25.0ml of remaining 700.0ml @21:50:42.98 Intake.select :open @21:50:45.15 Intake @15.8psia, vacuum from 10.0 to 13.0psi @21:50:45.16 SS.reconfigure :inhaling 0.91ml/s @21:50:45.21 SS.seek 25ml @21:51:14.96 Stroke completed -- waiting for pressure to equalize ... @21:51:14.97 SS.reconfigure :exhale @21:51:15.02 SS.hold @21:51:33.69 Intake.select :close @21:51:33.84 SSV.dial :exhaust @21:51:34.61 Exhaust.select :open @21:51:34.72 SS.seek 0ml @21:51:43.65 Exhaust.select :close @21:51:43.76 SSV.dial CBV @21:51:43.78
CBV.dial SSV @21:51:44.56 Allowing 130s for 25.0ml of remaining 675.0ml @21:51:44.57 Intake.select :open @21:51:46.74 Intake @15.8psia, vacuum from 10.0 to 13.0psi @21:51:46.75 SS.reconfigure :inhaling 0.91ml/s @21:51:46.80 SS.seek 25ml @21:52:16.32 Stroke completed -- waiting for pressure to equalize ... @21:52:16.33 SS.reconfigure :exhale @21:52:16.38 SS.hold @21:52:34.90 Intake.select :close @21:52:35.05 SSV.dial :exhaust @21:52:35.81 Exhaust.select :open @21:52:35.92 SS.seek 0ml @21:52:44.53 Exhaust.select :close @21:52:44.63 SSV.dial CBV @21:52:44.65
CBV.dial SSV @21:52:45.45 Allowing 130s for 25.0ml of remaining 650.0ml @21:52:45.46 Intake.select :open @21:52:47.63 Intake @15.8psia, vacuum from 10.0 to 13.0psi @21:52:47.65 SS.reconfigure :inhaling 0.91ml/s @21:52:47.69 SS.seek 25ml @21:53:17.32 Stroke completed -- waiting for pressure to equalize ... @21:53:17.33 SS.reconfigure :exhale @21:53:17.38 SS.hold @21:53:36.19 Intake.select :close @21:53:36.34 SSV.dial :exhaust @21:53:37.10 Exhaust.select :open @21:53:37.22 SS.seek 0ml @21:53:45.98 Exhaust.select :close @21:53:46.09 SSV.dial CBV @21:53:46.11
CBV.dial SSV @21:53:46.89 Allowing 130s for 25.0ml of remaining 625.0ml @21:53:46.90 Intake.select :open @21:53:49.06 Intake @15.9psia, vacuum from 10.0 to 13.0psi @21:53:49.07 SS.reconfigure :inhaling 0.91ml/s @21:53:49.12 SS.seek 25ml @21:54:18.58 Vacuum too high -- waiting up to 40 seconds @21:54:18.59 SS.reconfigure :exhale @21:54:18.64 SS.hold @21:54:26.65 Intake @16.5psia, vacuum from 10.0 to 13.0psi @21:54:26.66 SS.reconfigure :inhaling 0.73ml/s @21:54:26.71 SS.seek 25ml @21:54:26.93 Stroke completed -- waiting for pressure to equalize ... @21:54:26.94 SS.reconfigure :exhale @21:54:26.99 SS.hold @21:54:38.13 Intake.select :close @21:54:38.28 SSV.dial :exhaust @21:54:39.04 Exhaust.select :open @21:54:39.16 SS.seek 0ml @21:54:47.92 Exhaust.select :close @21:54:48.03 SSV.dial CBV @21:54:48.05
CBV.dial SSV @21:54:48.84 Allowing 130s for 25.0ml of remaining 600.0ml @21:54:48.85 Intake.select :open @21:54:51.01 Intake @15.8psia, vacuum from 10.0 to 13.0psi @21:54:51.02 SS.reconfigure :inhaling 0.73ml/s @21:54:51.07 SS.seek 25ml @21:55:27.46 Stroke completed -- waiting for pressure to equalize ... @21:55:27.47 SS.reconfigure :exhale @21:55:27.52 SS.hold @21:55:42.01 Intake.select :close @21:55:42.16 SSV.dial :exhaust @21:55:42.92 Exhaust.select :open @21:55:43.03 SS.seek 0ml @21:55:51.77 Exhaust.select :close @21:55:51.88 SSV.dial CBV @21:55:51.91
CBV.dial SSV @21:55:52.68 Allowing 130s for 25.0ml of remaining 575.0ml @21:55:52.69 Intake.select :open @21:55:54.86 Intake @15.8psia, vacuum from 10.0 to 13.0psi @21:55:54.88 SS.reconfigure :inhaling 0.73ml/s @21:55:54.93 SS.seek 25ml @21:56:31.21 Stroke completed -- waiting for pressure to equalize ... @21:56:31.22 SS.reconfigure :exhale @21:56:31.27 SS.hold @21:56:47.62 Intake.select :close @21:56:47.77 SSV.dial :exhaust @21:56:48.53 Exhaust.select :open @21:56:48.64 SS.seek 0ml @21:56:57.57 Exhaust.select :close @21:56:57.68 SSV.dial CBV @21:56:57.70
CBV.dial SSV @21:56:58.49 Allowing 130s for 25.0ml of remaining 550.0ml @21:56:58.50 Intake.select :open @21:57:00.67 Intake @15.7psia, vacuum from 10.0 to 13.0psi @21:57:00.68 SS.reconfigure :inhaling 0.73ml/s @21:57:00.73 SS.seek 25ml @21:57:36.66 Stroke completed -- waiting for pressure to equalize ... @21:57:36.67 SS.reconfigure :exhale @21:57:36.72 SS.hold @21:57:55.13 Intake.select :close @21:57:55.30 SSV.dial :exhaust @21:57:56.07 Exhaust.select :open @21:57:56.18 SS.seek 0ml @21:58:05.08 Exhaust.select :close @21:58:05.19 SSV.dial CBV @21:58:05.23
CBV.dial SSV @21:58:05.98 Allowing 130s for 25.0ml of remaining 525.0ml @21:58:05.99 Intake.select :open @21:58:08.15 Intake @16.0psia, vacuum from 10.0 to 13.0psi @21:58:08.16 SS.reconfigure :inhaling 0.73ml/s @21:58:08.21 SS.seek 25ml @21:58:44.36 Stroke completed -- waiting for pressure to equalize ... @21:58:44.37 SS.reconfigure :exhale @21:58:44.42 SS.hold @21:59:04.89 Intake.select :close @21:59:05.05 SSV.dial :exhaust @21:59:05.81 Exhaust.select :open @21:59:05.93 SS.seek 0ml @21:59:14.82 Exhaust.select :close @21:59:14.92 SSV.dial CBV @21:59:14.95
CBV.dial SSV @21:59:15.72 Allowing 130s for 25.0ml of remaining 500.0ml @21:59:15.73 Intake.select :open @21:59:17.90 Intake @16.0psia, vacuum from 10.0 to 13.0psi @21:59:17.92 SS.reconfigure :inhaling 0.73ml/s @21:59:17.97 SS.seek 25ml @21:59:54.03 Stroke completed -- waiting for pressure to equalize ... @21:59:54.04 SS.reconfigure :exhale @21:59:54.14 SS.hold @22:00:14.97 Intake.select :close @22:00:15.12 SSV.dial :exhaust @22:00:15.88 Exhaust.select :open @22:00:16.00 SS.seek 0ml @22:00:24.94 Exhaust.select :close @22:00:25.05 SSV.dial CBV @22:00:25.07
CBV.dial SSV @22:00:25.86 Allowing 130s for 25.0ml of remaining 475.0ml @22:00:25.87 Intake.select :open @22:00:28.04 Intake @15.9psia, vacuum from 10.0 to 13.0psi @22:00:28.06 SS.reconfigure :inhaling 0.73ml/s @22:00:28.11 SS.seek 25ml @22:01:04.19 Stroke completed -- waiting for pressure to equalize ... @22:01:04.20 SS.reconfigure :exhale @22:01:04.25 SS.hold @22:01:26.72 Intake.select :close @22:01:27.23 SSV.dial :exhaust @22:01:28.05 Exhaust.select :open @22:01:28.17 SS.seek 0ml @22:01:36.74 Exhaust.select :close @22:01:36.84 SSV.dial CBV @22:01:36.87
CBV.dial SSV @22:01:37.64 Allowing 130s for 25.0ml of remaining 450.0ml @22:01:37.65 Intake.select :open @22:01:39.82 Intake @15.9psia, vacuum from 10.0 to 13.0psi @22:01:39.84 SS.reconfigure :inhaling 0.73ml/s @22:01:39.88 SS.seek 25ml @22:02:16.31 Stroke completed -- waiting for pressure to equalize ... @22:02:16.32 SS.reconfigure :exhale @22:02:16.37 SS.hold @22:02:39.17 Intake.select :close @22:02:39.32 SSV.dial :exhaust @22:02:40.08 Exhaust.select :open @22:02:40.56 SS.seek 0ml @22:02:49.30 Exhaust.select :close @22:02:49.41 SSV.dial CBV @22:02:49.43
CBV.dial SSV @22:02:50.22 Allowing 130s for 25.0ml of remaining 425.0ml @22:02:50.23 Intake.select :open @22:02:52.40 Intake @16.0psia, vacuum from 10.0 to 13.0psi @22:02:52.42 SS.reconfigure :inhaling 0.73ml/s @22:02:52.46 SS.seek 25ml @22:03:28.70 Stroke completed -- waiting for pressure to equalize ... @22:03:28.71 SS.reconfigure :exhale @22:03:28.76 SS.hold @22:03:53.29 Intake.select :close @22:03:53.45 SSV.dial :exhaust @22:03:54.20 Exhaust.select :open @22:03:54.32 SS.seek 0ml @22:04:03.51 Exhaust.select :close @22:04:03.63 SSV.dial CBV @22:04:03.66
CBV.dial SSV @22:04:04.41 Allowing 130s for 25.0ml of remaining 400.0ml @22:04:04.42 Intake.select :open @22:04:06.58 Intake @16.0psia, vacuum from 10.0 to 13.0psi @22:04:06.60 SS.reconfigure :inhaling 0.73ml/s @22:04:06.65 SS.seek 25ml @22:04:43.07 Stroke completed -- waiting for pressure to equalize ... @22:04:43.09 SS.reconfigure :exhale @22:04:43.13 SS.hold @22:05:09.71 Intake.select :close @22:05:09.86 SSV.dial :exhaust @22:05:10.63 Exhaust.select :open @22:05:11.17 SS.seek 0ml @22:05:19.90 Exhaust.select :close @22:05:20.01 SSV.dial CBV @22:05:20.05
CBV.dial SSV @22:05:20.79 Allowing 130s for 25.0ml of remaining 375.0ml @22:05:20.81 Intake.select :open @22:05:22.98 Intake @16.0psia, vacuum from 10.0 to 13.0psi @22:05:22.99 SS.reconfigure :inhaling 0.73ml/s @22:05:23.04 SS.seek 25ml @22:05:49.03 Vacuum too high -- waiting up to 40 seconds @22:05:49.05 SS.reconfigure :exhale @22:05:49.10 SS.hold @22:05:57.78 Intake @16.5psia, vacuum from 10.0 to 13.0psi @22:05:57.79 SS.reconfigure :inhaling 0.59ml/s @22:05:57.84 SS.seek 25ml @22:06:11.14 Stroke completed -- waiting for pressure to equalize ... @22:06:11.15 SS.reconfigure :exhale @22:06:11.19 SS.hold @22:06:29.55 Intake.select :close @22:06:29.71 SSV.dial :exhaust @22:06:30.47 Exhaust.select :open @22:06:30.58 SS.seek 0ml @22:06:39.53 Exhaust.select :close @22:06:39.63 SSV.dial CBV @22:06:39.66
CBV.dial SSV @22:06:40.45 Allowing 130s for 25.0ml of remaining 350.0ml @22:06:40.46 Intake.select :open @22:06:42.63 Intake @16.2psia, vacuum from 10.0 to 13.0psi @22:06:42.64 SS.reconfigure :inhaling 0.59ml/s @22:06:42.69 SS.seek 25ml @22:07:26.75 Vacuum too high -- waiting up to 40 seconds @22:07:26.77 SS.reconfigure :exhale @22:07:26.82 SS.hold @22:07:33.50 Intake @15.6psia, vacuum from 10.0 to 13.0psi @22:07:33.51 SS.reconfigure :inhaling 0.47ml/s @22:07:33.56 SS.seek 25ml @22:07:34.21 Stroke completed -- waiting for pressure to equalize ... @22:07:34.22 SS.reconfigure :exhale @22:07:34.27 SS.hold @22:07:52.69 Intake.select :close @22:07:52.85 SSV.dial :exhaust @22:07:53.61 Exhaust.select :open @22:07:53.73 SS.seek 0ml @22:08:02.56 Exhaust.select :close @22:08:02.66 SSV.dial CBV @22:08:02.68
CBV.dial SSV @22:08:03.48 Allowing 130s for 25.0ml of remaining 325.0ml @22:08:03.49 Intake.select :open @22:08:05.66 Intake @16.1psia, vacuum from 10.0 to 13.0psi @22:08:05.67 SS.reconfigure :inhaling 0.47ml/s @22:08:05.72 SS.seek 25ml @22:09:01.94 Stroke completed -- waiting for pressure to equalize ... @22:09:01.96 SS.reconfigure :exhale @22:09:02.00 SS.hold @22:09:24.39 Intake.select :close @22:09:24.55 SSV.dial :exhaust @22:09:25.31 Exhaust.select :open @22:09:25.42 SS.seek 0ml @22:09:34.23 Exhaust.select :close @22:09:34.33 SSV.dial CBV @22:09:34.35
CBV.dial SSV @22:09:35.15 Allowing 130s for 25.0ml of remaining 300.0ml @22:09:35.16 Intake.select :open @22:09:37.33 Intake @16.1psia, vacuum from 10.0 to 13.0psi @22:09:37.34 SS.reconfigure :inhaling 0.47ml/s @22:09:37.39 SS.seek 25ml @22:10:32.90 Stroke completed -- waiting for pressure to equalize ... @22:10:32.91 SS.reconfigure :exhale @22:10:32.95 SS.hold @22:10:57.87 Intake.select :close @22:10:58.07 SSV.dial :exhaust @22:10:58.84 Exhaust.select :open @22:10:58.95 SS.seek 0ml @22:11:07.74 Exhaust.select :close @22:11:07.85 SSV.dial CBV @22:11:07.88
CBV.dial SSV @22:11:08.65 Allowing 130s for 25.0ml of remaining 275.0ml @22:11:08.66 Intake.select :open @22:11:10.83 Intake @16.1psia, vacuum from 10.0 to 13.0psi @22:11:10.85 SS.reconfigure :inhaling 0.47ml/s @22:11:10.90 SS.seek 25ml @22:12:06.52 Stroke completed -- waiting for pressure to equalize ... @22:12:06.53 SS.reconfigure :exhale @22:12:06.58 SS.hold @22:12:31.39 Intake.select :close @22:12:31.54 SSV.dial :exhaust @22:12:32.30 Exhaust.select :open @22:12:32.42 SS.seek 0ml @22:12:41.36 Exhaust.select :close @22:12:41.47 SSV.dial CBV @22:12:41.49
CBV.dial SSV @22:12:42.27 Allowing 130s for 25.0ml of remaining 250.0ml @22:12:42.28 Intake.select :open @22:12:44.45 Intake @16.2psia, vacuum from 10.0 to 13.0psi @22:12:44.47 SS.reconfigure :inhaling 0.47ml/s @22:12:44.52 SS.seek 25ml @22:13:39.61 Stroke completed -- waiting for pressure to equalize ... @22:13:39.62 SS.reconfigure :exhale @22:13:39.67 SS.hold @22:14:08.68 Intake.select :close @22:14:08.83 SSV.dial :exhaust @22:14:09.60 Exhaust.select :open @22:14:09.71 SS.seek 0ml @22:14:18.58 Exhaust.select :close @22:14:18.69 SSV.dial CBV @22:14:18.71
CBV.dial SSV @22:14:19.50 Allowing 130s for 25.0ml of remaining 225.0ml @22:14:19.51 Intake.select :open @22:14:21.68 Intake @16.3psia, vacuum from 10.0 to 13.0psi @22:14:21.70 SS.reconfigure :inhaling 0.47ml/s @22:14:21.74 SS.seek 25ml @22:15:17.63 Stroke completed -- waiting for pressure to equalize ... @22:15:17.64 SS.reconfigure :exhale @22:15:17.69 SS.hold @22:15:46.46 Intake.select :close @22:15:46.62 SSV.dial :exhaust @22:15:47.38 Exhaust.select :open @22:15:47.49 SS.seek 0ml @22:15:56.36 Exhaust.select :close @22:15:56.47 SSV.dial CBV @22:15:56.49
CBV.dial SSV @22:15:57.28 Allowing 130s for 25.0ml of remaining 200.0ml @22:15:57.29 Intake.select :open @22:15:59.89 Intake @16.1psia, vacuum from 10.0 to 13.0psi @22:15:59.90 SS.reconfigure :inhaling 0.47ml/s @22:15:59.95 SS.seek 25ml @22:16:55.14 Stroke completed -- waiting for pressure to equalize ... @22:16:55.15 SS.reconfigure :exhale @22:16:55.20 SS.hold @22:17:27.98 Intake.select :close @22:17:28.13 SSV.dial :exhaust @22:17:28.89 Exhaust.select :open @22:17:29.53 SS.seek 0ml @22:17:38.72 Exhaust.select :close @22:17:38.83 SSV.dial CBV @22:17:38.86
CBV.dial SSV @22:17:39.61 Allowing 130s for 25.0ml of remaining 175.0ml @22:17:39.62 Intake.select :open @22:17:41.79 Intake @16.2psia, vacuum from 10.0 to 13.0psi @22:17:41.81 SS.reconfigure :inhaling 0.47ml/s @22:17:41.85 SS.seek 25ml @22:18:36.40 Stroke completed -- waiting for pressure to equalize ... @22:18:36.41 SS.reconfigure :exhale @22:18:36.46 SS.hold @22:19:11.24 Intake.select :close @22:19:11.40 SSV.dial :exhaust @22:19:12.15 Exhaust.select :open @22:19:12.27 SS.seek 0ml @22:19:21.37 Exhaust.select :close @22:19:21.48 SSV.dial CBV @22:19:21.52
CBV.dial SSV @22:19:22.28 Allowing 130s for 25.0ml of remaining 150.0ml @22:19:22.29 Intake.select :open @22:19:24.46 Intake @16.2psia, vacuum from 10.0 to 13.0psi @22:19:24.47 SS.reconfigure :inhaling 0.47ml/s @22:19:24.52 SS.seek 25ml @22:20:13.64 Vacuum too high -- waiting up to 40 seconds @22:20:13.66 SS.reconfigure :exhale @22:20:13.71 SS.hold @22:20:32.22 Intake @18.3psia, vacuum from 10.0 to 13.0psi @22:20:32.24 SS.reconfigure :inhaling 0.38ml/s @22:20:32.72 SS.seek 25ml @22:20:39.92 Stroke completed -- waiting for pressure to equalize ... @22:20:39.93 SS.reconfigure :exhale @22:20:39.97 SS.hold @22:21:02.33 Intake.select :close @22:21:02.49 SSV.dial :exhaust @22:21:03.25 Exhaust.select :open @22:21:03.36 SS.seek 0ml @22:21:12.43 Exhaust.select :close @22:21:12.54 SSV.dial CBV @22:21:12.57
CBV.dial SSV @22:21:13.34 Allowing 130s for 25.0ml of remaining 125.0ml @22:21:13.35 Intake.select :open @22:21:15.52 Intake @16.1psia, vacuum from 10.0 to 13.0psi @22:21:15.53 SS.reconfigure :inhaling 0.38ml/s @22:21:15.58 SS.seek 25ml @22:22:23.55 Stroke completed -- waiting for pressure to equalize ... @22:22:23.56 SS.reconfigure :exhale @22:22:24.00 SS.hold @22:22:56.72 Intake.select :close @22:22:56.88 SSV.dial :exhaust @22:22:57.66 Exhaust.select :open @22:22:57.77 SS.seek 0ml @22:23:06.66 Exhaust.select :close @22:23:06.77 SSV.dial CBV @22:23:06.79
CBV.dial SSV @22:23:07.93 Allowing 130s for 25.0ml of remaining 100.0ml @22:23:07.94 Intake.select :open @22:23:10.11 Intake @16.3psia, vacuum from 10.0 to 13.0psi @22:23:10.13 SS.reconfigure :inhaling 0.38ml/s @22:23:10.18 SS.seek 25ml @22:24:18.71 Stroke completed -- waiting for pressure to equalize ... @22:24:18.72 SS.reconfigure :exhale @22:24:18.77 SS.hold @22:24:53.85 Intake.select :close @22:24:54.08 SSV.dial :exhaust @22:24:54.84 Exhaust.select :open @22:24:54.95 SS.seek 0ml @22:25:03.76 Exhaust.select :close @22:25:03.86 SSV.dial CBV @22:25:03.89
CBV.dial SSV @22:25:04.66 Allowing 130s for 25.0ml of remaining 75.0ml @22:25:04.68 Intake.select :open @22:25:06.84 Intake @16.3psia, vacuum from 10.0 to 13.0psi @22:25:06.86 SS.reconfigure :inhaling 0.38ml/s @22:25:06.90 SS.seek 25ml @22:26:15.52 Stroke completed -- waiting for pressure to equalize ... @22:26:15.53 SS.reconfigure :exhale @22:26:15.58 SS.hold @22:26:52.83 Intake.select :close @22:26:52.99 SSV.dial :exhaust @22:26:53.76 Exhaust.select :open @22:26:53.87 SS.seek 0ml @22:27:02.69 Exhaust.select :close @22:27:02.80 SSV.dial CBV @22:27:02.82
CBV.dial SSV @22:27:03.61 Allowing 130s for 25.0ml of remaining 50.0ml @22:27:03.63 Intake.select :open @22:27:05.80 Intake @16.3psia, vacuum from 10.0 to 13.0psi @22:27:05.81 SS.reconfigure :inhaling 0.38ml/s @22:27:05.86 SS.seek 25ml @22:28:14.36 Stroke completed -- waiting for pressure to equalize ... @22:28:14.37 SS.reconfigure :exhale @22:28:14.42 SS.hold @22:28:54.60 SS.reconfigure :default @22:28:54.65 SS.hold @22:28:54.72 Intake.select :close @22:28:54.88 ShallowSampler::Clogged in MAIN -- Intake @16psia & Outlet @15psia won't equalize after 975.0ml @22:28:54.89 SSV.dial :exhaust @22:28:55.66 Exhaust.select :open @22:28:55.77 SS.reconfigure :exhale @22:28:55.82 SS.seek 0ml @22:29:04.95 Exhaust.select :close @22:29:05.07 Sampled 975.0ml @22:29:05.08 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @22:29:05.09 Intake.select :close @22:29:05.16 CSV.dial :waste @22:29:05.32 SSV.dial CBV,avoiding: :exhaust @22:29:05.34 CS.seek 0ml @22:29:05.37
CBV.dial SSV @22:29:05.50 CSV.dial :air @22:29:06.24 CS.seek 10ml @22:29:07.27
CTV.dial :air @22:29:08.05 delay 15 seconds @22:29:23.07 SSV.dial :spit,avoiding: :exhaust @22:29:24.39 CTV.dial CSV,avoiding: :intake @22:29:24.41
SS.seek 0ml @22:29:24.55 SSV.dial CBV,avoiding: :exhaust @22:29:30.58 SSV.dial between CBV and plug @22:29:31.07 CBV.dial between SSV and waste2 @22:29:31.45 CSV.dial between air and CTV @22:29:31.76 CS.seek 8.319ml @22:29:36.83 CTV.dial CSV,avoiding: :air @22:29:36.85
CSV.dial CTV @22:29:37.21 CS.reconfigure :pressurize @22:29:37.26 CS.seek 0ml @22:29:52.66 BubblePoint: Intake @24.4psia, Outlet @1.1psia @22:29:52.67 CBV.dial SSV @22:29:52.99 SS.reconfigure :default @22:29:53.04 SS.seek 10ml @22:29:58.56 delay 15 seconds @22:30:13.70 Evacuated: Intake @23.7psia, Outlet @23.5psia @22:30:13.71 SSV.dial :spit,avoiding: :exhaust @22:30:14.74 SS.reconfigure :exhale @22:30:14.79 SS.seek 0ml @22:30:19.81 SS.reconfigure :fast @22:30:19.85 SS.seek 5ml @22:30:22.59 SSV.dial CBV,avoiding: :exhaust @22:30:22.61
CBV.dial SSV @22:30:23.94 CTV.dial :air,avoiding: :intake @22:30:25.29 CSV.dial CTV @22:30:25.33
SS.seek 10ml @22:30:26.45 CSV.dial :air @22:30:26.90 CS.reconfigure :fast @22:30:26.95 CS.seek 0ml @22:30:27.86
SSV.dial :spit,avoiding: :exhaust @22:30:29.18 SS.reconfigure :exhale @22:30:29.23 SS.seek 0ml @22:30:33.48 CS.stop @22:30:34.22
SS.reconfigure :fast @22:30:34.26 SS.seek 5ml @22:30:37.09 SSV.dial CBV,avoiding: :exhaust @22:30:37.11
CBV.dial SSV @22:30:39.45 SSV.dial :spit,avoiding: :exhaust @22:30:40.77 SS.reconfigure :default @22:30:40.82 SS.seek 0ml @22:30:44.08 SS.stop @22:30:44.12 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @22:30:44.13 Intake.select :close @22:30:44.20 CSV.dial :waste @22:30:44.32 SSV.dial CBV,avoiding: :exhaust @22:30:44.35
CBV.dial SSV @22:30:44.99 CS.seek 0ml @22:30:45.06 CSV.dial :air @22:30:45.69
CTV.dial :air @22:30:45.79 CS.seek 10ml @22:30:45.88
delay 15 seconds @22:31:00.90 SSV.dial :spit,avoiding: :exhaust @22:31:02.23 CTV.dial CSV,avoiding: :intake @22:31:02.26
SS.reconfigure :exhale @22:31:02.36 SS.seek 0ml @22:31:02.80 SSV.dial CBV,avoiding: :exhaust @22:31:10.20 SSV.dial between CBV and plug @22:31:10.74 CBV.dial between SSV and waste2 @22:31:11.14 CSV.dial between air and CTV @22:31:11.57 CS.seek 7.621ml @22:31:17.87 CTV.dial CSV,avoiding: :air @22:31:17.89
CSV.dial CTV @22:31:18.26 CS.reconfigure :pressurize @22:31:18.31 CS.seek 0ml @22:31:34.18 BubblePoint: Intake @24.3psia, Outlet @3.2psia @22:31:34.19 CBV.dial SSV @22:31:34.52 SS.reconfigure :default @22:31:34.57 SS.seek 10ml @22:31:40.19 delay 15 seconds @22:31:55.32 Evacuated: Intake @24.0psia, Outlet @23.7psia @22:31:55.33 SSV.dial :spit,avoiding: :exhaust @22:31:56.37 SS.reconfigure :exhale @22:31:56.41 SS.seek 0ml @22:32:01.57 SS.reconfigure :fast @22:32:01.62 SS.seek 5ml @22:32:04.38 SSV.dial CBV,avoiding: :exhaust @22:32:04.40
CBV.dial SSV @22:32:05.78 CTV.dial :air,avoiding: :intake @22:32:07.14 CSV.dial CTV @22:32:07.20
SS.seek 10ml @22:32:08.37 CSV.dial :air @22:32:08.80 CS.reconfigure :fast @22:32:08.85 CS.seek 0ml @22:32:09.70
SSV.dial :spit,avoiding: :exhaust @22:32:11.04 SS.reconfigure :exhale @22:32:11.09 SS.seek 0ml @22:32:13.45 CS.stop @22:32:16.06
SS.reconfigure :fast @22:32:16.10 SS.seek 5ml @22:32:18.87 SSV.dial CBV,avoiding: :exhaust @22:32:18.89
CBV.dial SSV @22:32:21.26 SSV.dial :spit,avoiding: :exhaust @22:32:22.57 SS.reconfigure :default @22:32:22.61 SS.seek 0ml @22:32:25.81 SS.stop @22:32:25.85 Lysing: Adding 2.0ml AqMeOH @22:32:25.86 CBV.dial between CSV and waste1,avoiding: :DBV @22:32:27.36 CTV.dial CSV @22:32:28.67 CSV.dial CSR @22:32:29.89 Pulling 1ml of CSR.AqMeOH @22:32:29.90 CSR.select :AqMeOH @22:32:30.01 CS.reconfigure :default @22:32:30.06 CS.seek 1ml @22:32:33.70 delay 5 seconds @22:32:38.72 CSR.select :air @22:32:38.90 CS.seek 6ml @22:32:52.55 delay 2 seconds @22:32:54.56 CSV.dial :waste2 @22:32:55.13 CS.reconfigure :fast @22:32:55.18 CS.seek 0ml @22:33:09.55 CSV.dial CSR @22:33:09.98 Pulling 2ml of CSR.AqMeOH @22:33:09.99 CSR.select :AqMeOH @22:33:10.11 CS.reconfigure :default @22:33:10.16 CS.seek 2ml @22:33:16.11 delay 5 seconds @22:33:21.13 CSR.select :air @22:33:21.29 CS.seek 5ml @22:33:29.95 delay 2 seconds @22:33:31.97 CSV.dial CTV @22:33:32.54 CS.seek 1ml @22:33:43.90 Before lysing Collection Puck Heater at 13.4C/30.8psia @22:33:43.92 Heating Puck.from 5, :sh1 in Collection Clamp to 70C for 10:00 @22:33:43.94 CH.seek 70C,8:00 @22:35:56.69 CH.hold 10:00,70C @22:35:56.88 Begun lysing Collection Puck Heater at 68.1C/37.7psia @22:45:56.88 Ended lysing Collection Puck Heater at 70C/36.5psia @22:45:56.90 CS.seek 0ml @22:46:00.41 CTV.dial between spare and air,avoiding: :intake @22:46:01.44 CSV.dial :air @22:46:01.92 CS.seek 5ml @22:46:15.69 CSV.dial CTV @22:46:16.15 delay 2 seconds @22:46:18.16 CSV.dial :air @22:46:18.61 delay 2 seconds @22:46:20.63 CSV.dial CTV @22:46:21.08 delay 2 seconds @22:46:23.10 CSV.dial :air @22:46:23.55 delay 2 seconds @22:46:25.57 CS.seek 0ml @22:46:39.75 Puck @80.4psia after final lysis pressurization @22:46:39.76 Recovering Lysate into Collection syringe @22:46:39.77 CSV.dial CBV @22:46:40.78 CBV.dial CSV @22:46:41.18 CS.seek 5ml @22:46:54.78 delay 5 seconds @22:46:59.80 CTV.dial :air @22:47:00.33 CS.seek 7.001ml @22:47:06.22 delay 5 seconds @22:47:11.24 CSV.dial :air2 @22:47:11.70 CS.seek 10ml @22:47:20.22 CSV.dial between air and CTV,avoiding: CBV @22:47:21.38 Lysing Complete @22:47:21.45 Storing sh1 puck in Collection Clamp to tube 4 @22:47:21.63 SC.reconfigure :tight @22:47:21.68 SC.seek 4 @22:47:25.29 SC.configure :limp @22:47:25.50 Elbow.reconfigure :tight @22:47:25.55 Elbow.seek 4 @22:47:27.31 Forearm.reconfigure :tight @22:47:27.36 Forearm.seek 4 + 450 counts @22:47:28.39 SE.reconfigure :pick @22:47:28.45 SE.seek :beamBreak @22:47:30.05 SE.configure :default @22:47:30.21 Elbow.reconfigure :limp @22:47:30.26 Forearm.reconfigure :loose @22:47:30.31 Forearm.seek 4 @22:47:30.58 Elbow.hold @22:47:30.64 Elbow.reconfigure :tight @22:47:30.74 SE.seek 24.1 Pucks below Top Plate @22:47:31.27 Forearm.force -20 @22:47:31.31 Forearm.seek 4 + 293 counts @22:47:31.60 Forearm.reconfigure :tight @22:47:31.65 Forearm.seek 4 + 450 counts @22:47:31.83 SE.seek 25.1 Pucks below Top Plate @22:47:32.90 Forearm.reconfigure :loose @22:47:32.95 Forearm.seek :retract @22:47:33.88 Elbow.seek CC @22:47:35.54 Elbow.reconfigure :loose @22:47:35.64 Forearm.seek CC @22:47:36.62 Hand.close @22:47:37.22 CC.seek :open @22:47:41.96 Forearm.seek :retract @22:47:42.97 Elbow.reconfigure :tight @22:47:43.02 Elbow.seek 4 @22:47:44.79 Elbow.reconfigure :limp @22:47:44.84 Forearm.seek 4 @22:47:45.83 Elbow.hold @22:47:45.89 Elbow.reconfigure :tight @22:47:45.94 Hand.open @22:47:46.71 Forearm.force -20 @22:47:46.76 Forearm.seek 4 + 309 counts @22:47:47.57 Forearm.reconfigure :tight @22:47:47.62 Forearm.seek 4 + 450 counts @22:47:47.94 SE.seek :down @22:47:48.95 SE.stop @22:47:49.11
Forearm.reconfigure :loose @22:47:49.16 Forearm.seek :retract @22:47:50.08 Tranferring DA @22:47:50.09 Beginning samplePassC @22:47:50.10 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @22:47:50.29 Elbow.seek FlushPuck::Garage @22:47:52.10 Elbow.reconfigure :loose @22:47:52.20 Forearm.seek :garage @22:47:53.23 Hand.close @22:47:53.97 Forearm.seek :retract @22:47:54.96 Elbow.reconfigure :tight @22:47:55.00 Elbow.seek CC @22:47:57.91 Elbow.reconfigure :loose @22:47:58.01 Forearm.reconfigure :tight @22:47:58.06 Forearm.seek CC @22:47:59.31 CC.seek :closed @22:48:03.82 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.40 @22:48:03.89 Hand.open @22:48:04.53 Forearm.reconfigure :loose @22:48:04.58 Forearm.seek :retract @22:48:05.56 PSV.dial PSR @22:48:07.08 Pulling 1.5ml of PSR.flush @22:48:07.09 PSR.select :flush @22:48:07.21 PS.seek 1.5ml @22:48:23.32 delay 5 seconds @22:48:28.34 PSV.dial :waste1 @22:48:28.81 PS.seek 0ml @22:48:44.97 PSV.dial PSR @22:48:45.44 PSR.select :air @22:48:45.56 PS.seek 2.5ml @22:49:12.01 PRV.dial CBV,avoiding: :ab2 @22:49:12.03
CBV.dial PRV @22:49:13.78 PSV.dial :mixloop @22:49:14.83 CTV.dial :air,avoiding: :intake @22:49:14.85 PTV.dial :PRVmixing @22:49:16.20 PS.seek 0ml @22:49:42.94 PSV.dial :air @22:49:43.40 PS.seek 2.5ml @22:50:09.84 PSV.dial :mixloop @22:50:10.32 PS.seek 0ml @22:50:36.66 PSV.dial :air @22:50:37.13 PS.seek 2.5ml @22:51:03.50 PSV.dial :mixloop @22:51:03.98 PS.seek 0ml @22:51:30.38 Garaging FlushPuck in Collection Clamp @22:51:30.45 Elbow.reconfigure :tight @22:51:30.50 Elbow.seek CC @22:51:30.82 Elbow.reconfigure :loose @22:51:30.92 Forearm.seek CC @22:51:31.98 Hand.close @22:51:32.59 CC.seek :open @22:51:37.24 Forearm.seek :retract @22:51:38.30 Elbow.reconfigure :tight @22:51:38.34 Elbow.seek :garage @22:51:41.26 Elbow.reconfigure :loose @22:51:41.36 Forearm.reconfigure :tight @22:51:41.41 Forearm.seek :garage @22:51:42.49 Hand.open @22:51:43.15 Forearm.reconfigure :loose @22:51:43.20 Forearm.seek :retract @22:51:44.20 PSV.dial :mixloop @22:51:44.28 PTV.dial :PRVmixing @22:51:44.36 PRV.dial CBV @22:51:44.38
CBV.dial PRV @22:51:44.47 CTV.dial :air,avoiding: :intake @22:51:44.49 Loading lyfil puck from tube 5 into Collection Clamp @22:51:44.72 SC.reconfigure :tight @22:51:44.76 SC.seek 5 @22:51:48.30 SC.configure :limp @22:51:48.96 Elbow.reconfigure :tight @22:51:49.00 Elbow.seek 5 @22:51:50.95 Forearm.reconfigure :tight @22:51:51.00 Forearm.seek 5 + 450 counts @22:51:52.09 SE.reconfigure :pick @22:51:52.15 SE.seek :beamBreak @22:51:54.87 SE.configure :default @22:51:55.04 Elbow.seek 5 @22:51:55.22 Forearm.seek 5 + 300 counts @22:51:55.46 Forearm.reconfigure :loose @22:51:55.51 Hand.close @22:51:56.10 SE.seek :down @22:51:56.32
Forearm.seek :retract @22:51:57.29 Elbow.seek CC @22:51:58.49 SE.stop @22:51:58.91
Elbow.reconfigure :loose @22:51:59.01 Forearm.reconfigure :tight @22:51:59.06 Forearm.seek CC @22:52:00.27 CC.seek :closed @22:52:04.79 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.27 @22:52:04.80 Hand.open @22:52:05.53 Forearm.reconfigure :loose @22:52:05.96 Forearm.seek :retract @22:52:07.00 CTV.dial CSV,avoiding: :intake @22:52:08.36 CSV.dial CTV @22:52:08.69 PS.seek 2.5ml @22:52:35.06 CS.seek 4.5ml @22:52:49.96 delay 8 seconds @22:52:57.99 PSV.dial :air @22:52:58.45 delay 2 seconds @22:53:00.47 PSV.dial :waste2 @22:53:01.21 PS.seek 0ml @22:53:27.56 CBV.dial between PRV and waste1 @22:53:27.89 CSV.dial :air @22:53:28.36 CS.seek 10ml @22:53:43.46 CSV.dial CTV @22:53:43.92 CS.seek 0ml @22:54:10.40 CBV.dial :waste1 @22:54:10.71 delay 5 seconds @22:54:15.74 CTV.dial :air,avoiding: :intake @22:54:17.15 Storing lyfil puck in Collection Clamp to tube 4 @22:54:17.33 SC.reconfigure :tight @22:54:17.37 SC.seek 4 @22:54:20.91 SC.configure :limp @22:54:21.13 Elbow.reconfigure :tight @22:54:21.18 Elbow.seek 4 @22:54:23.06 Forearm.reconfigure :tight @22:54:23.11 Forearm.seek 4 + 450 counts @22:54:24.21 SE.reconfigure :pick @22:54:24.27 SE.seek :beamBreak @22:54:25.76 SE.configure :default @22:54:25.93 Elbow.reconfigure :limp @22:54:25.98 Forearm.reconfigure :loose @22:54:26.03 Forearm.seek 4 @22:54:26.32 Elbow.hold @22:54:26.39 Elbow.reconfigure :tight @22:54:26.92 SE.seek 25.11 Pucks below Top Plate @22:54:27.51 Forearm.force -20 @22:54:27.56 Forearm.seek 4 + 282 counts @22:54:27.85 Forearm.reconfigure :tight @22:54:27.90 Forearm.seek 4 + 450 counts @22:54:28.09 SE.seek 26.11 Pucks below Top Plate @22:54:28.73 Forearm.reconfigure :loose @22:54:28.78 Forearm.seek :retract @22:54:29.72 Elbow.seek CC @22:54:31.34 Elbow.reconfigure :loose @22:54:31.44 Forearm.seek CC @22:54:32.43 Hand.close @22:54:33.09 CC.seek :open @22:54:37.82 Forearm.seek :retract @22:54:38.81 Elbow.reconfigure :tight @22:54:38.86 Elbow.seek 4 @22:54:40.61 Elbow.reconfigure :limp @22:54:40.66 Forearm.seek 4 @22:54:41.71 Elbow.hold @22:54:41.78 Elbow.reconfigure :tight @22:54:41.83 Hand.open @22:54:42.59 Forearm.force -20 @22:54:42.64 Forearm.seek 4 + 303 counts @22:54:43.03 Forearm.reconfigure :tight @22:54:43.08 Forearm.seek 4 + 450 counts @22:54:43.84 SE.seek :down @22:54:44.80 SE.stop @22:54:44.98
Forearm.reconfigure :loose @22:54:45.03 Forearm.seek :retract @22:54:45.95 PSV.dial :air @22:54:46.71 PS.seek 2.5ml @22:55:13.12 delay 1 second @22:55:14.14 PSV.dial :mixloop @22:55:14.59 delay 1 second @22:55:15.61 PSV.dial :air @22:55:16.07 delay 1 second @22:55:17.09 PSV.dial :mixloop @22:55:17.56 delay 1 second @22:55:18.57 PSV.dial :air @22:55:19.04 delay 1 second @22:55:20.06 PS.seek 0ml @22:55:46.47 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @22:55:46.65 Elbow.seek FlushPuck::Garage @22:55:48.49 Elbow.reconfigure :loose @22:55:48.59 Forearm.seek :garage @22:55:49.62 Hand.close @22:55:50.33 Forearm.seek :retract @22:55:51.37 Elbow.reconfigure :tight @22:55:51.42 Elbow.seek PC @22:55:52.69 Elbow.reconfigure :loose @22:55:52.79 Forearm.reconfigure :tight @22:55:52.84 Forearm.seek PC @22:55:54.13 PC.seek :closed @22:55:58.93 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.65 @22:55:59.00 Hand.open @22:55:59.65 Forearm.reconfigure :loose @22:55:59.70 Forearm.seek :retract @22:56:00.65 PRV.dial :air @22:56:01.12 PSV.dial :mixloop @22:56:01.65 PS.seek 0.3ml @22:56:05.62 PSV.dial PSR @22:56:06.79 Pulling 0.4ml of PSR.air @22:56:06.80 PSR.select :air @22:56:06.82 PS.seek 0.7ml @22:56:11.68 delay 3 seconds @22:56:14.70 PSR.select :air @22:56:14.72 PS.seek 2.5ml @22:56:33.93 delay 3 seconds @22:56:36.95 PSV.dial :mixloop @22:56:37.98 PTV.dial :mixing @22:56:38.74 PBV.dial :waste2 @22:56:39.71 PS.seek 0ml @22:57:06.09 Garaging FlushPuck in Processing Clamp @22:57:06.16 Elbow.reconfigure :tight @22:57:06.21 Elbow.seek PC @22:57:06.33 Elbow.reconfigure :loose @22:57:06.43 Forearm.seek PC @22:57:07.40 Hand.close @22:57:07.96 PC.seek :open @22:57:12.67 Forearm.seek :retract @22:57:13.62 Elbow.reconfigure :tight @22:57:13.67 Elbow.seek :garage @22:57:15.08 Elbow.reconfigure :loose @22:57:15.18 Forearm.reconfigure :tight @22:57:15.23 Forearm.seek :garage @22:57:16.26 Hand.open @22:57:16.90 Forearm.reconfigure :loose @22:57:16.94 Forearm.seek :retract @22:57:17.98 Loading da puck from tube 5 into Processing Clamp @22:57:18.59 SC.reconfigure :tight @22:57:18.70 SC.seek 5 @22:57:22.27 SC.configure :limp @22:57:22.49 Elbow.reconfigure :tight @22:57:22.54 Elbow.seek 5 @22:57:24.38 Forearm.reconfigure :tight @22:57:24.43 Forearm.seek 5 + 450 counts @22:57:25.48 SE.reconfigure :pick @22:57:25.53 SE.seek :beamBreak @22:57:28.30 SE.configure :default @22:57:28.47 Elbow.seek 5 @22:57:28.65 Forearm.seek 5 + 300 counts @22:57:28.85 Forearm.reconfigure :loose @22:57:28.90 Hand.close @22:57:29.52 SE.seek :down @22:57:29.72
Forearm.seek :retract @22:57:30.67 Elbow.seek PC @22:57:31.97 SE.stop @22:57:33.20
Elbow.reconfigure :loose @22:57:33.31 Forearm.reconfigure :tight @22:57:33.36 Forearm.seek PC @22:57:34.56 PC.seek :closed @22:57:39.19 Processing Clamp onSpring/offSpring for 15/25 samples, on/off torque 1.63 @22:57:39.20 Hand.open @22:57:40.29 Forearm.reconfigure :loose @22:57:40.41 Forearm.seek :retract @22:57:41.36 Heating Puck.from 5, :da in Processing Clamp to 28C for 2:00:00 @22:57:41.38 PH.seek 28C,5:00 @22:58:24.69 PH.hold 2:00:00,28C @22:58:24.74 delay 1:30 @22:59:54.77 PSR.select :air @22:59:54.79 PSV.dial PSR @22:59:55.85 Pulling 0.5ml of PSR.wash @22:59:55.86 PSR.select :wash @22:59:55.98 PS.seek 0.5ml @23:00:01.90 delay 5 seconds @23:00:06.91 PSR.select :air @23:00:07.04 PS.seek 2ml @23:00:23.21 PTV.dial :puck @23:00:24.82 PSV.dial PTV @23:00:26.02 PBV.dial :waste1 @23:00:27.41 PS.seek 1.5ml @23:00:33.40 delay 3 seconds @23:00:36.41 PBV.dial between PSV and waste1 @23:00:36.78 PS.seek 0ml @23:00:52.93 delay 5:00 @23:05:52.95 recoverPSRreagent08 @23:05:52.96 PTV.dial :puck @23:05:53.03 PBV.dial :waste1 @23:05:53.41 delay 30 seconds @23:06:23.43 PBV.dial between PSV and waste1 @23:06:23.79 PSR.select :air @23:06:23.81 PSV.dial PSR @23:06:24.57 PS.reconfigure :fast @23:06:24.62 PS.seek 2.5ml @23:06:48.32 PSV.dial PTV @23:06:49.09 delay 5 seconds @23:06:54.11 PSV.dial PSR @23:06:54.86 delay 5 seconds @23:06:59.88 PSV.dial PTV @23:07:00.62 delay 5 seconds @23:07:05.64 PSV.dial :air @23:07:06.43 PS.seek 0ml @23:07:29.58 PTV.dial :PRVmixing,via: PRV @23:07:30.90 PSV.dial :mixloop @23:07:31.37 PRV.dial :ab1 @23:07:32.13 PS.reconfigure :slow @23:07:32.17 PS.seek 0.15ml @23:07:35.76 delay 2 seconds @23:07:37.78 CBV.dial PRV @23:07:37.80
PRV.dial CBV @23:07:38.38 PS.seek 0.35ml @23:07:42.98 delay 2 seconds @23:07:45.00 CBV.dial between PRV and PSV @23:07:45.31 PRV.dial :air @23:07:45.77 PS.seek 0.9ml @23:07:57.48 PRV.dial :waste2 @23:07:58.24 PS.seek 0.5ml @23:08:06.89 addPMLReagent08 @23:08:06.90 PSV.dial :air @23:08:07.36 PS.reconfigure :default @23:08:07.41 PS.seek 2ml @23:08:23.60 PSV.dial :PML @23:08:24.07 PTV.dial :mixing @23:08:24.82 PBV.dial :waste1 @23:08:25.24 PS.seek 1.85ml @23:08:27.63 PBV.dial between PSV and waste1 @23:08:28.00 PS.seek 0ml @23:08:48.20 delay 2 seconds @23:08:50.22 Beginning samplePassC @23:08:50.23 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @23:08:50.42 Elbow.reconfigure :tight @23:08:50.47 Elbow.seek FlushPuck::Garage @23:08:51.85 Elbow.reconfigure :loose @23:08:51.95 Forearm.seek :garage @23:08:52.90 Hand.close @23:08:53.70 Forearm.seek :retract @23:08:54.67 Elbow.reconfigure :tight @23:08:54.72 Elbow.seek CC @23:08:57.57 Elbow.reconfigure :loose @23:08:57.67 Forearm.reconfigure :tight @23:08:57.72 Forearm.seek CC @23:08:58.92 CC.seek :closed @23:09:03.45 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.40 @23:09:03.53 Hand.open @23:09:04.14 Forearm.reconfigure :loose @23:09:04.18 Forearm.seek :retract @23:09:05.17 PSV.dial PSR @23:09:06.23 Pulling 1.5ml of PSR.flush @23:09:06.24 PSR.select :flush @23:09:06.36 PS.seek 1.5ml @23:09:22.51 delay 5 seconds @23:09:27.53 PSV.dial :waste1 @23:09:28.00 PS.seek 0ml @23:09:44.20 PSV.dial PSR @23:09:45.10 PSR.select :air @23:09:45.22 PS.seek 2.5ml @23:10:11.58 PRV.dial CBV,avoiding: :ab2 @23:10:11.60
CBV.dial PRV @23:10:13.23 PSV.dial :mixloop @23:10:14.28 CTV.dial :air,avoiding: :intake @23:10:14.35 PTV.dial :PRVmixing @23:10:15.12 PS.seek 0ml @23:10:41.51 PSV.dial :air @23:10:42.09 PS.seek 2.5ml @23:11:08.44 PSV.dial :mixloop @23:11:08.90 PS.seek 0ml @23:11:35.44 PSV.dial :air @23:11:35.92 PS.seek 2.5ml @23:12:02.22 PSV.dial :mixloop @23:12:02.70 PS.seek 0ml @23:12:29.14 Garaging FlushPuck in Collection Clamp @23:12:29.21 Elbow.reconfigure :tight @23:12:29.26 Elbow.seek CC @23:12:29.55 Elbow.reconfigure :loose @23:12:29.65 Forearm.seek CC @23:12:30.65 Hand.close @23:12:31.33 CC.seek :open @23:12:35.94 Forearm.seek :retract @23:12:36.90 Elbow.reconfigure :tight @23:12:36.95 Elbow.seek :garage @23:12:39.90 Elbow.reconfigure :loose @23:12:40.00 Forearm.reconfigure :tight @23:12:40.05 Forearm.seek :garage @23:12:41.09 Hand.open @23:12:41.76 Forearm.reconfigure :loose @23:12:41.80 Forearm.seek :retract @23:12:42.79 delayUntil "23:16:50.21" @23:12:42.82 Whole Cell Archive sampling 1000ml @23:12:42.84 Loading wcr puck from tube 5 into Collection Clamp @23:12:43.52 SC.reconfigure :tight @23:12:43.57 SC.seek 5 @23:12:43.68 SC.configure :limp @23:12:43.90 Elbow.reconfigure :tight @23:12:43.95 Elbow.seek 5 @23:12:45.91 Forearm.reconfigure :tight @23:12:45.97 Forearm.seek 5 + 450 counts @23:12:47.03 SE.reconfigure :pick @23:12:47.09 SE.seek :beamBreak @23:12:49.93 SE.configure :default @23:12:50.10 Elbow.seek 5 @23:12:50.29 Forearm.seek 5 + 300 counts @23:12:50.49 Forearm.reconfigure :loose @23:12:50.54 Hand.close @23:12:51.21 SE.seek :down @23:12:51.42 Forearm.seek :retract @23:12:52.39 Elbow.seek CC @23:12:53.72 SE.stop @23:12:54.05 Elbow.reconfigure :loose @23:12:54.16 Forearm.reconfigure :tight @23:12:54.21 Forearm.seek CC @23:12:55.50 CC.seek :closed @23:13:00.02 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.34 @23:13:00.03 Hand.open @23:13:00.76 Forearm.reconfigure :loose @23:13:00.81 Forearm.seek :retract @23:13:01.81 collectSample 1000ml w/bubble point of 35psi @23:13:01.82 Priming 7ml @23:13:01.83 Intake.select :close @23:13:01.84 Exhaust.select :close @23:13:01.86 SS.hold @23:13:02.08 Calibrating pressure sensors @23:13:02.09 CTV.dial :air @23:13:02.17 SSV.dial :spit,avoiding: :exhaust @23:13:02.25 SS.reconfigure :fast @23:13:02.31 SS.seek 5ml @23:13:05.02 SSV.dial CBV @23:13:05.04 CBV.dial SSV @23:13:06.42 delay 3.8 seconds @23:13:10.36 Sensor limits: Intake max 102.2psia; Outlet max 93.8psia @23:13:10.37 SSV.dial :spit @23:13:11.71 SS.seek 0ml @23:13:14.59 SSV.dial CBV @23:13:15.92 CTV.dial :intake @23:13:16.70 SSV.dial CBV @23:13:16.72 CBV.dial SSV @23:13:16.88 Allowing 40s for 7.0ml of remaining 7.0ml @23:13:16.89 Intake.select :open @23:13:19.07 Intake @14.3psia, vacuum from 8.8 to 11.8psi @23:13:19.08 SS.reconfigure :inhaling 2.20ml/s @23:13:19.13 SS.seek 7ml @23:13:25.76 Stroke completed -- waiting for pressure to equalize ... @23:13:25.77 SS.reconfigure :exhale @23:13:25.82 SS.hold @23:13:48.66 Intake.select :close @23:13:48.82 SSV.dial :exhaust @23:13:49.59 Exhaust.select :open @23:13:49.71 SS.seek 0ml @23:13:53.76 Exhaust.select :close @23:13:53.88 Sampling 1000.0ml @23:13:53.90 Intake.select :close @23:13:53.91 Exhaust.select :close @23:13:53.92 SS.reconfigure :default @23:13:53.96 SS.hold @23:13:54.18 CTV.dial :intake @23:13:54.42 SSV.dial CBV @23:13:54.44 CBV.dial SSV @23:13:55.22 Allowing 130s for 25.0ml of remaining 1000.0ml @23:13:55.23 Intake.select :open @23:13:57.83 Intake @15.4psia, vacuum from 9.9 to 12.9psi @23:13:57.86 SS.reconfigure :inhaling 2.20ml/s @23:13:57.92 SS.seek 25ml @23:14:05.89 Vacuum too high -- waiting up to 40 seconds @23:14:05.91 SS.reconfigure :exhale @23:14:05.97 SS.hold @23:14:14.94 Intake @19.3psia, vacuum from 10.0 to 13.0psi @23:14:14.96 SS.reconfigure :inhaling 1.76ml/s @23:14:15.01 SS.seek 25ml @23:14:17.05 Vacuum too high -- waiting up to 40 seconds @23:14:17.07 SS.reconfigure :exhale @23:14:17.12 SS.hold @23:14:18.97 Intake @18.5psia, vacuum from 10.0 to 13.0psi @23:14:18.99 SS.reconfigure :inhaling 1.41ml/s @23:14:19.03 SS.seek 25ml @23:14:22.59 Vacuum too high -- waiting up to 40 seconds @23:14:22.61 SS.reconfigure :exhale @23:14:22.66 SS.hold @23:14:26.37 Intake @19.0psia, vacuum from 10.0 to 13.0psi @23:14:26.39 SS.reconfigure :inhaling 1.13ml/s @23:14:26.44 SS.seek 25ml @23:14:29.39 Vacuum too high -- waiting up to 40 seconds @23:14:29.41 SS.reconfigure :exhale @23:14:29.46 SS.hold @23:14:31.60 Intake @19.7psia, vacuum from 10.0 to 13.0psi @23:14:31.62 SS.reconfigure :inhaling 0.91ml/s @23:14:31.66 SS.seek 25ml @23:14:34.24 Vacuum too high -- waiting up to 40 seconds @23:14:34.25 SS.reconfigure :exhale @23:14:34.31 SS.hold @23:14:36.48 Intake @18.7psia, vacuum from 10.0 to 13.0psi @23:14:36.50 SS.reconfigure :inhaling 0.73ml/s @23:14:36.55 SS.seek 25ml @23:14:41.51 Stroke completed -- waiting for pressure to equalize ... @23:14:41.52 SS.reconfigure :exhale @23:14:41.57 SS.hold @23:15:12.20 Intake.select :close @23:15:12.36 SSV.dial :exhaust @23:15:13.14 Exhaust.select :open @23:15:13.26 SS.seek 0ml @23:15:22.10 Exhaust.select :close @23:15:22.21 SSV.dial CBV @23:15:22.23 CBV.dial SSV @23:15:23.01 Allowing 130s for 25.0ml of remaining 975.0ml @23:15:23.03 Intake.select :open @23:15:25.19 Intake @15.9psia, vacuum from 10.0 to 13.0psi @23:15:25.21 SS.reconfigure :inhaling 0.73ml/s @23:15:25.26 SS.seek 25ml @23:16:01.74 Stroke completed -- waiting for pressure to equalize ... @23:16:01.76 SS.reconfigure :exhale @23:16:01.81 SS.hold @23:16:38.64 Intake.select :close @23:16:38.80 SSV.dial :exhaust @23:16:39.56 Exhaust.select :open @23:16:39.67 SS.seek 0ml @23:16:48.73 Exhaust.select :close @23:16:48.84 SSV.dial CBV @23:16:48.87 CBV.dial SSV @23:16:49.63 Allowing 130s for 25.0ml of remaining 950.0ml @23:16:49.65 Intake.select :open @23:16:50.21 recoverPRVreagent08 @23:16:50.23 PTV.dial :mixing @23:16:50.98 PBV.dial :waste1 @23:16:51.36 delay 30 seconds @23:16:51.82 Intake @15.9psia, vacuum from 10.0 to 13.0psi @23:16:51.84 SS.reconfigure :inhaling 0.73ml/s @23:16:51.89 SS.seek 25ml @23:17:21.38 PBV.dial between PSV and waste1 @23:17:21.77 PSV.dial :air @23:17:22.38 PS.reconfigure :fast @23:17:22.43 PS.seek 2.5ml @23:17:28.02 Stroke completed -- waiting for pressure to equalize ... @23:17:28.03 SS.reconfigure :exhale @23:17:28.08 SS.hold @23:17:46.34 PSV.dial :mixloop @23:17:46.82 delay 2 seconds @23:17:48.84 PSV.dial :air @23:17:49.44 delay 2 seconds @23:17:51.45 PSV.dial :mixloop @23:17:51.91 delay 2 seconds @23:17:53.92 PSV.dial :air @23:17:54.40 PS.seek 0ml @23:18:03.17 Intake.select :close @23:18:03.34 SSV.dial :exhaust @23:18:04.10 Exhaust.select :open @23:18:04.22 SS.seek 0ml @23:18:13.30 Exhaust.select :close @23:18:13.41 SSV.dial CBV @23:18:13.44 CBV.dial SSV @23:18:14.21 Allowing 130s for 25.0ml of remaining 925.0ml @23:18:14.22 Intake.select :open @23:18:16.39 Intake @16.0psia, vacuum from 10.0 to 13.0psi @23:18:16.41 SS.reconfigure :inhaling 0.73ml/s @23:18:16.46 SS.seek 25ml @23:18:18.16 washCycleDA #1 of 2 @23:18:18.17 PSR.select :air @23:18:18.18 PSV.dial PSR @23:18:19.53 Pulling 0.5ml of PSR.wash @23:18:19.54 PSR.select :wash @23:18:19.66 PS.reconfigure :default @23:18:19.71 PS.seek 0.5ml @23:18:25.65 delay 5 seconds @23:18:30.68 PSR.select :air @23:18:30.80 PS.seek 2ml @23:18:46.90 PTV.dial :puck @23:18:47.65 PSV.dial PTV @23:18:48.40 PBV.dial :waste1 @23:18:48.84 PS.seek 1.5ml @23:18:52.61 Stroke completed -- waiting for pressure to equalize ... @23:18:52.62 SS.reconfigure :exhale @23:18:52.67 SS.hold @23:18:54.81 delay 3 seconds @23:18:57.83 PBV.dial between PSV and waste1 @23:18:58.22 PS.seek 0ml @23:19:14.41 delay 2:00 @23:19:25.45 Intake.select :close @23:19:25.61 SSV.dial :exhaust @23:19:26.39 Exhaust.select :open @23:19:26.51 SS.seek 0ml @23:19:35.55 Exhaust.select :close @23:19:35.66 SSV.dial CBV @23:19:35.70 CBV.dial SSV @23:19:36.47 Allowing 130s for 25.0ml of remaining 900.0ml @23:19:36.49 Intake.select :open @23:19:39.11 Intake @15.9psia, vacuum from 10.0 to 13.0psi @23:19:39.13 SS.reconfigure :inhaling 0.73ml/s @23:19:39.18 SS.seek 25ml @23:20:15.16 Stroke completed -- waiting for pressure to equalize ... @23:20:15.17 SS.reconfigure :exhale @23:20:15.22 SS.hold @23:20:42.15 Intake.select :close @23:20:42.31 SSV.dial :exhaust @23:20:43.08 Exhaust.select :open @23:20:43.20 SS.seek 0ml @23:20:52.03 Exhaust.select :close @23:20:52.14 SSV.dial CBV @23:20:52.19 CBV.dial SSV @23:20:52.93 Allowing 130s for 25.0ml of remaining 875.0ml @23:20:52.94 Intake.select :open @23:20:55.11 Intake @16.0psia, vacuum from 10.0 to 13.0psi @23:20:55.13 SS.reconfigure :inhaling 0.73ml/s @23:20:55.18 SS.seek 25ml @23:21:14.43 recoverPSRreagent08 @23:21:14.45 PTV.dial :puck @23:21:14.64 PBV.dial :waste1 @23:21:15.01 delay 30 seconds @23:21:31.67 Stroke completed -- waiting for pressure to equalize ... @23:21:31.69 SS.reconfigure :exhale @23:21:31.73 SS.hold @23:21:45.03 PBV.dial between PSV and waste1 @23:21:45.39 PSR.select :air @23:21:45.40 PSV.dial PSR @23:21:46.17 PS.reconfigure :fast @23:21:46.22 PS.seek 2.5ml @23:21:58.30 Intake.select :close @23:21:58.46 SSV.dial :exhaust @23:21:59.23 Exhaust.select :open @23:21:59.35 SS.seek 0ml @23:22:08.39 Exhaust.select :close @23:22:08.51 SSV.dial CBV @23:22:08.55 CBV.dial SSV @23:22:09.30 Allowing 130s for 25.0ml of remaining 850.0ml @23:22:09.31 Intake.select :open @23:22:10.27 PSV.dial PTV @23:22:11.17 delay 5 seconds @23:22:11.48 Intake @16.0psia, vacuum from 10.0 to 13.0psi @23:22:11.50 SS.reconfigure :inhaling 0.73ml/s @23:22:11.55 SS.seek 25ml @23:22:16.18 PSV.dial PSR @23:22:16.93 delay 5 seconds @23:22:21.95 PSV.dial PTV @23:22:22.71 delay 5 seconds @23:22:27.74 PSV.dial :air @23:22:28.64 PS.seek 0ml @23:22:47.53 Stroke completed -- waiting for pressure to equalize ... @23:22:47.54 SS.reconfigure :exhale @23:22:47.59 SS.hold @23:22:51.99 washCycleDA #2 of 2 @23:22:52.00 PSR.select :air @23:22:52.02 PSV.dial PSR @23:22:53.38 Pulling 0.5ml of PSR.wash @23:22:53.40 PSR.select :wash @23:22:53.58 PS.reconfigure :default @23:22:53.63 PS.seek 0.5ml @23:22:59.52 delay 5 seconds @23:23:04.54 PSR.select :air @23:23:04.65 PS.seek 2ml @23:23:12.31 Intake.select :close @23:23:12.47 SSV.dial :exhaust @23:23:13.23 Exhaust.select :open @23:23:13.35 SS.seek 0ml @23:23:20.93 PTV.dial :puck @23:23:21.01 PSV.dial PTV @23:23:21.77 PBV.dial :waste1 @23:23:22.29 Exhaust.select :close @23:23:22.41 SSV.dial CBV @23:23:22.44 CBV.dial SSV @23:23:22.68 PS.seek 1.5ml @23:23:23.22 Allowing 130s for 25.0ml of remaining 825.0ml @23:23:23.24 Intake.select :open @23:23:25.40 Intake @16.0psia, vacuum from 10.0 to 13.0psi @23:23:25.42 SS.reconfigure :inhaling 0.73ml/s @23:23:25.47 SS.seek 25ml @23:23:28.65 delay 3 seconds @23:23:31.67 PBV.dial between PSV and waste1 @23:23:32.05 PS.seek 0ml @23:23:48.18 delay 2:00 @23:24:02.01 Stroke completed -- waiting for pressure to equalize ... @23:24:02.03 SS.reconfigure :exhale @23:24:02.07 SS.hold @23:24:26.65 Intake.select :close @23:24:26.81 SSV.dial :exhaust @23:24:27.58 Exhaust.select :open @23:24:27.70 SS.seek 0ml @23:24:36.70 Exhaust.select :close @23:24:36.82 SSV.dial CBV @23:24:36.84 CBV.dial SSV @23:24:37.61 Allowing 130s for 25.0ml of remaining 800.0ml @23:24:37.62 Intake.select :open @23:24:39.79 Intake @16.1psia, vacuum from 10.0 to 13.0psi @23:24:39.81 SS.reconfigure :inhaling 0.73ml/s @23:24:39.86 SS.seek 25ml @23:25:15.70 Stroke completed -- waiting for pressure to equalize ... @23:25:15.71 SS.reconfigure :exhale @23:25:15.76 SS.hold @23:25:40.31 Intake.select :close @23:25:40.47 SSV.dial :exhaust @23:25:41.23 Exhaust.select :open @23:25:41.35 SS.seek 0ml @23:25:48.21 recoverPSRreagent08 @23:25:48.22 PTV.dial :puck @23:25:48.29 PBV.dial :waste1 @23:25:48.66 delay 30 seconds @23:25:50.04 Exhaust.select :close @23:25:50.15 SSV.dial CBV @23:25:50.18 CBV.dial SSV @23:25:50.95 Allowing 130s for 25.0ml of remaining 775.0ml @23:25:50.97 Intake.select :open @23:25:53.14 Intake @16.0psia, vacuum from 10.0 to 13.0psi @23:25:53.16 SS.reconfigure :inhaling 0.73ml/s @23:25:53.20 SS.seek 25ml @23:26:18.68 PBV.dial between PSV and waste1 @23:26:19.05 PSR.select :air @23:26:19.07 PSV.dial PSR @23:26:19.84 PS.reconfigure :fast @23:26:19.89 PS.seek 2.5ml @23:26:29.68 Stroke completed -- waiting for pressure to equalize ... @23:26:29.69 SS.reconfigure :exhale @23:26:29.74 SS.hold @23:26:43.74 PSV.dial PTV @23:26:44.50 delay 5 seconds @23:26:49.52 PSV.dial PSR @23:26:50.29 delay 5 seconds @23:26:54.53 Intake.select :close @23:26:54.69 SSV.dial :exhaust @23:26:55.30 PSV.dial PTV @23:26:55.46 Exhaust.select :open @23:26:55.58 SS.seek 0ml @23:26:56.07 delay 5 seconds @23:27:01.09 PSV.dial :air @23:27:01.85 PS.seek 0ml @23:27:04.40 Exhaust.select :close @23:27:04.52 SSV.dial CBV @23:27:04.54 CBV.dial SSV @23:27:05.79 Allowing 130s for 25.0ml of remaining 750.0ml @23:27:05.81 Intake.select :open @23:27:07.98 Intake @16.0psia, vacuum from 10.0 to 13.0psi @23:27:08.00 SS.reconfigure :inhaling 0.73ml/s @23:27:08.05 SS.seek 25ml @23:27:25.52 getPMLReagent08 @23:27:25.53 PRV.dial :air @23:27:26.01 delay 5 seconds @23:27:31.03 PTV.dial :PRVmixing @23:27:32.35 delay 5 seconds @23:27:37.37 PRV.dial :ab2 @23:27:37.83 PTV.dial :PRVmixing @23:27:37.91 PSV.dial :mixloop @23:27:38.51 PS.reconfigure :default @23:27:38.56 PS.seek 0.3ml @23:27:42.43 delay 2 seconds @23:27:43.57 Vacuum too high -- waiting up to 40 seconds @23:27:43.58 SS.reconfigure :exhale @23:27:43.64 SS.hold @23:27:44.45 PRV.dial :air @23:27:45.04 PS.seek 0.55ml @23:27:48.49 addPMLReagent08 @23:27:48.50 PSV.dial :air @23:27:49.09 PS.seek 2ml @23:27:55.03 Intake @16.7psia, vacuum from 10.0 to 13.0psi @23:27:55.05 SS.reconfigure :inhaling 0.59ml/s @23:27:55.10 SS.seek 25ml @23:27:56.18 Stroke completed -- waiting for pressure to equalize ... @23:27:56.19 SS.reconfigure :exhale @23:27:56.24 SS.hold @23:28:04.67 PSV.dial :PML @23:28:05.14 PTV.dial :mixing @23:28:05.91 PBV.dial :waste1 @23:28:06.34 PS.seek 1.85ml @23:28:08.68 PBV.dial between PSV and waste1 @23:28:09.04 PS.seek 0ml @23:28:10.69 Intake.select :close @23:28:11.26 SSV.dial :exhaust @23:28:12.04 Exhaust.select :open @23:28:12.16 SS.seek 0ml @23:28:21.02 Exhaust.select :close @23:28:21.14 SSV.dial CBV @23:28:21.18 CBV.dial SSV @23:28:21.92 Allowing 130s for 25.0ml of remaining 725.0ml @23:28:21.93 Intake.select :open @23:28:24.10 Intake @16.1psia, vacuum from 10.0 to 13.0psi @23:28:24.12 SS.reconfigure :inhaling 0.59ml/s @23:28:24.18 SS.seek 25ml @23:28:28.80 delay 2 seconds @23:28:30.82 delay 8:00 @23:29:09.22 Stroke completed -- waiting for pressure to equalize ... @23:29:09.24 SS.reconfigure :exhale @23:29:09.29 SS.hold @23:29:29.72 Intake.select :close @23:29:29.88 SSV.dial :exhaust @23:29:30.66 Exhaust.select :open @23:29:30.78 SS.seek 0ml @23:29:39.54 Exhaust.select :close @23:29:39.65 SSV.dial CBV @23:29:39.70 CBV.dial SSV @23:29:40.45 Allowing 130s for 25.0ml of remaining 700.0ml @23:29:40.46 Intake.select :open @23:29:42.63 Intake @16.1psia, vacuum from 10.0 to 13.0psi @23:29:42.65 SS.reconfigure :inhaling 0.59ml/s @23:29:42.70 SS.seek 25ml @23:30:27.40 Stroke completed -- waiting for pressure to equalize ... @23:30:27.41 SS.reconfigure :exhale @23:30:27.46 SS.hold @23:30:51.98 Intake.select :close @23:30:52.14 SSV.dial :exhaust @23:30:52.93 Exhaust.select :open @23:30:53.05 SS.seek 0ml @23:31:01.88 Exhaust.select :close @23:31:02.00 SSV.dial CBV @23:31:02.02 CBV.dial SSV @23:31:02.82 Allowing 130s for 25.0ml of remaining 675.0ml @23:31:02.84 Intake.select :open @23:31:05.00 Intake @15.9psia, vacuum from 10.0 to 13.0psi @23:31:05.02 SS.reconfigure :inhaling 0.59ml/s @23:31:05.07 SS.seek 25ml @23:31:49.96 Stroke completed -- waiting for pressure to equalize ... @23:31:49.97 SS.reconfigure :exhale @23:31:50.02 SS.hold @23:32:15.04 Intake.select :close @23:32:15.20 SSV.dial :exhaust @23:32:15.94 Exhaust.select :open @23:32:16.06 SS.seek 0ml @23:32:24.83 Exhaust.select :close @23:32:24.95 SSV.dial CBV @23:32:24.97 CBV.dial SSV @23:32:25.74 Allowing 130s for 25.0ml of remaining 650.0ml @23:32:25.76 Intake.select :open @23:32:27.92 Intake @16.1psia, vacuum from 10.0 to 13.0psi @23:32:27.94 SS.reconfigure :inhaling 0.59ml/s @23:32:27.99 SS.seek 25ml @23:33:07.06 Vacuum too high -- waiting up to 40 seconds @23:33:07.07 SS.reconfigure :exhale @23:33:07.13 SS.hold @23:33:18.46 Intake @16.9psia, vacuum from 10.0 to 13.0psi @23:33:18.48 SS.reconfigure :inhaling 0.47ml/s @23:33:18.53 SS.seek 25ml @23:33:25.64 Stroke completed -- waiting for pressure to equalize ... @23:33:25.65 SS.reconfigure :exhale @23:33:25.70 SS.hold @23:33:40.58 Intake.select :close @23:33:40.74 SSV.dial :exhaust @23:33:41.52 Exhaust.select :open @23:33:41.64 SS.seek 0ml @23:33:50.77 Exhaust.select :close @23:33:50.88 SSV.dial CBV @23:33:50.90 CBV.dial SSV @23:33:51.69 Allowing 130s for 25.0ml of remaining 625.0ml @23:33:51.71 Intake.select :open @23:33:53.87 Intake @16.1psia, vacuum from 10.0 to 13.0psi @23:33:53.89 SS.reconfigure :inhaling 0.47ml/s @23:33:53.94 SS.seek 25ml @23:34:49.23 Stroke completed -- waiting for pressure to equalize ... @23:34:49.25 SS.reconfigure :exhale @23:34:49.29 SS.hold @23:35:13.89 Intake.select :close @23:35:14.04 SSV.dial :exhaust @23:35:14.83 Exhaust.select :open @23:35:14.95 SS.seek 0ml @23:35:23.95 Exhaust.select :close @23:35:24.06 SSV.dial CBV @23:35:24.10 CBV.dial SSV @23:35:24.88 Allowing 130s for 25.0ml of remaining 600.0ml @23:35:24.89 Intake.select :open @23:35:27.06 Intake @16.0psia, vacuum from 10.0 to 13.0psi @23:35:27.08 SS.reconfigure :inhaling 0.47ml/s @23:35:27.13 SS.seek 25ml @23:36:22.14 Stroke completed -- waiting for pressure to equalize ... @23:36:22.15 SS.reconfigure :exhale @23:36:22.20 SS.hold @23:36:30.84 recoverPRVreagent08 @23:36:30.85 PTV.dial :mixing @23:36:30.91 PBV.dial :waste1 @23:36:31.29 delay 30 seconds @23:36:47.23 Intake.select :close @23:36:47.40 SSV.dial :exhaust @23:36:48.17 Exhaust.select :open @23:36:48.29 SS.seek 0ml @23:36:57.12 Exhaust.select :close @23:36:57.23 SSV.dial CBV @23:36:57.25 CBV.dial SSV @23:36:58.05 Allowing 130s for 25.0ml of remaining 575.0ml @23:36:58.06 Intake.select :open @23:37:00.24 Intake @16.2psia, vacuum from 10.0 to 13.0psi @23:37:00.26 SS.reconfigure :inhaling 0.47ml/s @23:37:00.30 SS.seek 25ml @23:37:01.31 PBV.dial between PSV and waste1 @23:37:01.68 PSV.dial :air @23:37:02.30 PS.reconfigure :fast @23:37:02.82 PS.seek 2.5ml @23:37:26.76 PSV.dial :mixloop @23:37:27.21 delay 2 seconds @23:37:29.23 PSV.dial :air @23:37:29.70 delay 2 seconds @23:37:31.72 PSV.dial :mixloop @23:37:32.31 delay 2 seconds @23:37:34.32 PSV.dial :air @23:37:34.92 PS.seek 0ml @23:37:55.73 Stroke completed -- waiting for pressure to equalize ... @23:37:55.74 SS.reconfigure :exhale @23:37:55.79 SS.hold @23:37:58.35 washCycleDA #1 of 3 @23:37:58.36 PSR.select :air @23:37:58.38 PSV.dial PSR @23:37:59.73 Pulling 0.5ml of PSR.wash @23:37:59.74 PSR.select :wash @23:37:59.95 PS.reconfigure :default @23:38:00.02 PS.seek 0.5ml @23:38:05.94 delay 5 seconds @23:38:10.96 PSR.select :air @23:38:11.54 PS.seek 2ml @23:38:22.35 Intake.select :close @23:38:22.51 SSV.dial :exhaust @23:38:23.29 Exhaust.select :open @23:38:23.41 SS.seek 0ml @23:38:27.72 PTV.dial :puck @23:38:28.48 PSV.dial PTV @23:38:29.37 PBV.dial :waste1 @23:38:29.80 PS.seek 1.5ml @23:38:32.21 Exhaust.select :close @23:38:32.33 SSV.dial CBV @23:38:32.37 CBV.dial SSV @23:38:33.11 Allowing 130s for 25.0ml of remaining 550.0ml @23:38:33.12 Intake.select :open @23:38:35.30 Intake @16.1psia, vacuum from 10.0 to 13.0psi @23:38:35.32 SS.reconfigure :inhaling 0.47ml/s @23:38:35.36 SS.seek 25ml @23:38:35.79 delay 3 seconds @23:38:38.80 PBV.dial between PSV and waste1 @23:38:39.18 PS.seek 0ml @23:38:55.32 delay 2:00 @23:39:30.88 Stroke completed -- waiting for pressure to equalize ... @23:39:30.90 SS.reconfigure :exhale @23:39:30.95 SS.hold @23:40:01.70 Intake.select :close @23:40:01.86 SSV.dial :exhaust @23:40:02.64 Exhaust.select :open @23:40:02.75 SS.seek 0ml @23:40:11.56 Exhaust.select :close @23:40:11.67 SSV.dial CBV @23:40:11.69 CBV.dial SSV @23:40:12.48 Allowing 130s for 25.0ml of remaining 525.0ml @23:40:12.49 Intake.select :open @23:40:14.66 Intake @16.2psia, vacuum from 10.0 to 13.0psi @23:40:14.68 SS.reconfigure :inhaling 0.47ml/s @23:40:14.73 SS.seek 25ml @23:40:55.35 recoverPSRreagent08 @23:40:55.36 PTV.dial :puck @23:40:55.83 PBV.dial :waste1 @23:40:56.27 delay 30 seconds @23:40:59.80 Vacuum too high -- waiting up to 40 seconds @23:40:59.82 SS.reconfigure :exhale @23:40:59.87 SS.hold @23:41:10.51 Intake @16.4psia, vacuum from 10.0 to 13.0psi @23:41:10.53 SS.reconfigure :inhaling 0.38ml/s @23:41:10.58 SS.seek 25ml @23:41:23.90 Stroke completed -- waiting for pressure to equalize ... @23:41:23.91 SS.reconfigure :exhale @23:41:23.96 SS.hold @23:41:26.70 PBV.dial between PSV and waste1 @23:41:27.14 PSR.select :air @23:41:27.16 PSV.dial PSR @23:41:27.95 PS.reconfigure :fast @23:41:28.02 PS.seek 2.5ml @23:41:44.98 Intake.select :close @23:41:45.14 SSV.dial :exhaust @23:41:45.88 Exhaust.select :open @23:41:46.00 SS.seek 0ml @23:41:52.03 PSV.dial PTV @23:41:52.80 delay 5 seconds @23:41:54.84 Exhaust.select :close @23:41:54.95 SSV.dial CBV @23:41:54.98 CBV.dial SSV @23:41:55.74 Allowing 130s for 25.0ml of remaining 500.0ml @23:41:55.76 Intake.select :open @23:41:57.82 PSV.dial PSR @23:41:57.93 Intake @16.2psia, vacuum from 10.0 to 13.0psi @23:41:57.95 SS.reconfigure :inhaling 0.38ml/s @23:41:58.00 SS.seek 25ml @23:41:58.60 delay 5 seconds @23:42:03.62 PSV.dial PTV @23:42:04.85 delay 5 seconds @23:42:09.87 PSV.dial :air @23:42:10.76 PS.seek 0ml @23:42:34.13 washCycleDA #2 of 3 @23:42:34.14 PSR.select :air @23:42:34.16 PSV.dial PSR @23:42:35.50 Pulling 0.5ml of PSR.wash @23:42:35.51 PSR.select :wash @23:42:35.63 PS.reconfigure :default @23:42:35.68 PS.seek 0.5ml @23:42:41.60 delay 5 seconds @23:42:46.62 PSR.select :air @23:42:46.74 PS.seek 2ml @23:43:02.97 PTV.dial :puck @23:43:03.05 PSV.dial PTV @23:43:03.81 PBV.dial :waste1 @23:43:04.25 PS.seek 1.5ml @23:43:06.21 Stroke completed -- waiting for pressure to equalize ... @23:43:06.22 SS.reconfigure :exhale @23:43:06.27 SS.hold @23:43:10.12 delay 3 seconds @23:43:13.14 PBV.dial between PSV and waste1 @23:43:13.52 PS.seek 0ml @23:43:29.76 delay 2:00 @23:43:34.83 Intake.select :close @23:43:34.99 SSV.dial :exhaust @23:43:35.76 Exhaust.select :open @23:43:35.88 SS.seek 0ml @23:43:45.11 Exhaust.select :close @23:43:45.22 SSV.dial CBV @23:43:45.27 CBV.dial SSV @23:43:46.02 Allowing 130s for 25.0ml of remaining 475.0ml @23:43:46.04 Intake.select :open @23:43:48.21 Intake @16.2psia, vacuum from 10.0 to 13.0psi @23:43:48.23 SS.reconfigure :inhaling 0.38ml/s @23:43:48.28 SS.seek 25ml @23:44:55.57 Stroke completed -- waiting for pressure to equalize ... @23:44:55.58 SS.reconfigure :exhale @23:44:55.63 SS.hold @23:45:29.78 recoverPSRreagent08 @23:45:29.80 PTV.dial :puck @23:45:29.87 PBV.dial :waste1 @23:45:30.25 delay 30 seconds @23:45:30.46 Intake.select :close @23:45:30.62 SSV.dial :exhaust @23:45:31.40 Exhaust.select :open @23:45:31.51 SS.seek 0ml @23:45:40.39 Exhaust.select :close @23:45:40.50 SSV.dial CBV @23:45:40.53 CBV.dial SSV @23:45:41.30 Allowing 130s for 25.0ml of remaining 450.0ml @23:45:41.32 Intake.select :open @23:45:43.89 Intake @16.1psia, vacuum from 10.0 to 13.0psi @23:45:43.98 SS.reconfigure :inhaling 0.38ml/s @23:45:44.03 SS.seek 25ml @23:46:00.27 PBV.dial between PSV and waste1 @23:46:00.64 PSR.select :air @23:46:00.66 PSV.dial PSR @23:46:01.43 PS.reconfigure :fast @23:46:01.48 PS.seek 2.5ml @23:46:25.37 PSV.dial PTV @23:46:26.14 delay 5 seconds @23:46:31.16 PSV.dial PSR @23:46:31.92 delay 5 seconds @23:46:36.94 PSV.dial PTV @23:46:37.70 delay 5 seconds @23:46:42.72 PSV.dial :air @23:46:43.63 PS.seek 0ml @23:46:52.80 Stroke completed -- waiting for pressure to equalize ... @23:46:52.81 SS.reconfigure :exhale @23:46:52.86 SS.hold @23:47:07.09 washCycleDA #3 of 3 @23:47:07.10 PSR.select :air @23:47:07.12 PSV.dial PSR @23:47:08.47 Pulling 0.5ml of PSR.wash @23:47:08.48 PSR.select :wash @23:47:08.60 PS.reconfigure :default @23:47:08.65 PS.seek 0.5ml @23:47:14.56 delay 5 seconds @23:47:19.58 PSR.select :air @23:47:19.69 PS.seek 2ml @23:47:30.17 Intake.select :close @23:47:30.33 SSV.dial :exhaust @23:47:31.08 Exhaust.select :open @23:47:31.20 SS.seek 0ml @23:47:35.87 PTV.dial :puck @23:47:35.95 PSV.dial PTV @23:47:36.71 PBV.dial :waste1 @23:47:37.15 PS.seek 1.5ml @23:47:40.22 Exhaust.select :close @23:47:40.33 SSV.dial CBV @23:47:40.38 CBV.dial SSV @23:47:41.14 Allowing 130s for 25.0ml of remaining 425.0ml @23:47:41.15 Intake.select :open @23:47:43.12 delay 3 seconds @23:47:43.32 Intake @16.2psia, vacuum from 10.0 to 13.0psi @23:47:43.34 SS.reconfigure :inhaling 0.38ml/s @23:47:43.39 SS.seek 25ml @23:47:46.14 PBV.dial between PSV and waste1 @23:47:46.58 PS.seek 0ml @23:48:02.94 delay 2:00 @23:48:51.01 Vacuum too high -- waiting up to 40 seconds @23:48:51.03 SS.reconfigure :exhale @23:48:51.49 SS.hold @23:49:11.97 Intake @18.0psia, vacuum from 10.0 to 13.0psi @23:49:11.98 SS.reconfigure :inhaling 0.31ml/s @23:49:12.03 SS.seek 25ml @23:49:12.59 Stroke completed -- waiting for pressure to equalize ... @23:49:12.60 SS.reconfigure :exhale @23:49:12.65 SS.hold @23:49:35.46 Intake.select :close @23:49:35.62 SSV.dial :exhaust @23:49:36.39 Exhaust.select :open @23:49:36.51 SS.seek 0ml @23:49:45.61 Exhaust.select :close @23:49:45.72 SSV.dial CBV @23:49:45.77 CBV.dial SSV @23:49:46.52 Allowing 130s for 25.0ml of remaining 400.0ml @23:49:46.53 Intake.select :open @23:49:48.70 Intake @16.1psia, vacuum from 10.0 to 13.0psi @23:49:49.19 SS.reconfigure :inhaling 0.31ml/s @23:49:49.25 SS.seek 25ml @23:50:02.95 recoverPSRreagent08 @23:50:02.97 PTV.dial :puck @23:50:03.04 PBV.dial :waste1 @23:50:03.42 delay 30 seconds @23:50:33.44 PBV.dial between PSV and waste1 @23:50:33.81 PSR.select :air @23:50:33.83 PSV.dial PSR @23:50:34.59 PS.reconfigure :fast @23:50:34.64 PS.seek 2.5ml @23:50:58.46 PSV.dial PTV @23:50:59.35 delay 5 seconds @23:51:04.38 PSV.dial PSR @23:51:05.12 delay 5 seconds @23:51:10.14 PSV.dial PTV @23:51:10.92 delay 5 seconds @23:51:15.78 Stroke completed -- waiting for pressure to equalize ... @23:51:15.80 SS.reconfigure :exhale @23:51:15.85 SS.hold @23:51:15.93 PSV.dial :air @23:51:16.81 PS.seek 0ml @23:51:40.21 Gate.power :camera,:ON @23:51:40.22 Mixing DA Substrates @23:51:40.23 PSR.select :air @23:51:40.25 PSV.dial PSR @23:51:41.67 Pulling 0.25ml of PSR.sub1 @23:51:41.69 PSR.select :sub1 @23:51:41.90 PS.reconfigure :default @23:51:42.01 PS.seek 0.25ml @23:51:45.43 delay 5 seconds @23:51:50.45 Pulling 0.25ml of PSR.sub2 @23:51:50.46 PSR.select :sub2 @23:51:50.57 PS.seek 0.5ml @23:51:50.74 Intake.select :close @23:51:50.90 SSV.dial :exhaust @23:51:51.69 Exhaust.select :open @23:51:51.81 SS.seek 0ml @23:51:53.99 delay 5 seconds @23:51:59.01 PSR.select :air @23:51:59.13 PS.seek 2ml @23:52:00.76 Exhaust.select :close @23:52:00.87 SSV.dial CBV @23:52:00.92 CBV.dial SSV @23:52:01.67 Allowing 130s for 25.0ml of remaining 375.0ml @23:52:01.69 Intake.select :open @23:52:03.85 Intake @16.1psia, vacuum from 10.0 to 13.0psi @23:52:03.87 SS.reconfigure :inhaling 0.31ml/s @23:52:03.92 SS.seek 25ml @23:52:15.33 PTV.dial :puck @23:52:15.41 PSV.dial PTV @23:52:16.19 PBV.dial :waste1 @23:52:16.63 PS.seek 1.5ml @23:52:22.62 delay 3 seconds @23:52:25.64 PBV.dial between PSV and waste1 @23:52:26.01 PS.seek 0ml @23:52:42.59 delay 10 seconds @23:52:52.61 recoverPSRreagent08 @23:52:52.62 PTV.dial :puck @23:52:52.69 PBV.dial :waste1 @23:52:53.07 delay 30 seconds @23:53:23.10 PBV.dial between PSV and waste1 @23:53:23.48 PSR.select :air @23:53:23.49 PSV.dial PSR @23:53:24.26 PS.reconfigure :fast @23:53:24.31 PS.seek 2.5ml @23:53:31.79 Stroke completed -- waiting for pressure to equalize ... @23:53:31.80 SS.reconfigure :exhale @23:53:31.85 SS.hold @23:53:48.58 PSV.dial PTV @23:53:49.35 delay 5 seconds @23:53:54.44 PSV.dial PSR @23:53:55.23 delay 5 seconds @23:54:00.24 PSV.dial PTV @23:54:01.02 delay 5 seconds @23:54:06.04 PSV.dial :air @23:54:06.81 PS.seek 0ml @23:54:11.70 SS.reconfigure :default @23:54:11.76 SS.hold @23:54:11.83 Intake.select :close @23:54:11.99 ShallowSampler::Clogged in WCR -- Filtering too Slowly after 650.0ml @23:54:12.00 SSV.dial :exhaust @23:54:12.78 Exhaust.select :open @23:54:12.90 SS.reconfigure :exhale @23:54:12.95 SS.seek 0ml @23:54:22.17 Exhaust.select :close @23:54:22.28 Sampled 650.0ml @23:54:22.30 Drying puck before fixing for 5:00 @23:54:22.31 CBV.dial :waste2b @23:54:23.09 delay 1:00 @23:54:30.74 Additional air clear @23:54:30.75 PSR.select :air @23:54:30.77 PSV.dial PSR @23:54:32.13 Pulling 2ml of PSR.air @23:54:32.14 PSR.select :air @23:54:32.16 PS.reconfigure :default @23:54:32.21 PS.seek 2ml @23:54:53.49 delay 5 seconds @23:54:58.51 PTV.dial :puck @23:54:58.60 PSV.dial PTV @23:54:59.36 PBV.dial :waste1 @23:54:59.80 PS.seek 1.5ml @23:55:05.68 delay 3 seconds @23:55:08.70 PBV.dial between PSV and waste1 @23:55:09.07 delay 1 second @23:55:10.09 PSV.dial :waste1 @23:55:11.17 PS.seek 0ml @23:55:23.12 CSV.dial :air @23:55:23.68 CS.reconfigure :fast @23:55:23.73 CS.seek 10ml @23:55:27.51 Processing Puck Heater at 28.1C before imaging @23:55:27.52 PH.coast @23:55:27.64 Imaging da puck in Processing Clamp while storing it to tube 4 @23:55:27.82 SC.reconfigure :tight @23:55:27.87 SC.seek 4 @23:55:31.46 SC.configure :limp @23:55:31.67 Elbow.reconfigure :tight @23:55:31.73 Elbow.seek 4 @23:55:33.48 Forearm.reconfigure :tight @23:55:33.53 Forearm.seek 4 + 450 counts @23:55:34.58 SE.reconfigure :pick @23:55:34.64 SE.seek :beamBreak @23:55:36.09 SE.configure :default @23:55:36.25 Elbow.reconfigure :limp @23:55:36.30 Forearm.reconfigure :loose @23:55:36.35 Forearm.seek 4 @23:55:36.61 Elbow.hold @23:55:36.67 Elbow.reconfigure :tight @23:55:36.77 SE.seek 26.1 Pucks below Top Plate @23:55:37.32 Forearm.force -20 @23:55:37.37 Forearm.seek 4 + 300 counts @23:55:37.65 Forearm.reconfigure :tight @23:55:37.70 Forearm.seek 4 + 450 counts @23:55:37.90 SE.seek 27.1 Pucks below Top Plate @23:55:38.51 Forearm.reconfigure :loose @23:55:38.56 Forearm.seek :retract @23:55:39.52 Elbow.seek PC @23:55:42.20 Elbow.reconfigure :loose @23:55:42.31 Forearm.seek PC @23:55:43.24 Hand.close @23:55:43.92 PC.seek :open @23:55:48.53 Pumping wcr puck from tube 5 up to 35psi... @23:55:48.55 CBV.dial SSV @23:55:49.23 Forearm.seek :retract @23:55:49.85 CBV.dial between CSV and waste2b @23:55:50.15 Elbow.reconfigure :tight @23:55:50.20 Elbow.seek 4 @23:55:50.84 CSV.dial between air and CTV @23:55:51.16 CS.seek 7.022ml @23:55:52.81 Forearm.reconfigure :tight @23:55:52.86 Forearm.seek 4 + 450 counts @23:55:53.81 Hand.open @23:55:54.48 SE.seek 26.75 Pucks below Top Plate @23:55:54.74 SE.reconfigure :seal @23:55:54.79 SE.seek 25.79 Pucks below Top Plate @23:55:55.30 Lights, camera, ACTION! @23:55:55.34 PuckCamera: snap -bin=2,2 -auto /var/log/esp/da23dec0623h975ml.tif @23:55:58.98 CTV.dial CSV,avoiding: :air @23:55:59.01 CSV.dial CTV @23:55:59.86 CS.reconfigure :pressurize @23:55:59.92 CS.seek 0ml @23:56:13.46 CBV.dial :waste2b @23:56:13.81 CS.reconfigure :default @23:56:13.87 CS.seek 0ml @23:56:14.84 Waiting >20 seconds for da to relinquish FlushPuck @23:56:16.10 SX HX9/16 (USB:19): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 17.473 seconds /var/log/esp/da23dec0623h975ml.tif: TIFF Upload Complete @23:56:16.13 PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da23dec0623h975ml15s.tif @23:56:19.47 delay 1:00 @23:56:31.75 SX HX9/16 (USB:19): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15 seconds /var/log/esp/midres/da23dec0623h975ml15s.tif: TIFF Upload Complete @23:56:31.78 PuckCamera: snap -bin=2,2 20.0 /var/log/esp/midres/da23dec0623h975ml20s.tif @23:56:52.39 SX HX9/16 (USB:19): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 20 seconds /var/log/esp/midres/da23dec0623h975ml20s.tif: TIFF Upload Complete @23:56:52.40 Gate.power :camera,:OFF @23:56:52.42 DAfans.email "17.473 sec auto-exposure",Subject: "da23dec0623h975ml images!" @23:56:52.85 Elbow.seek 4 @23:56:53.08 Forearm.seek 4 + 450 counts @23:56:53.16 SE.reconfigure :gently @23:56:53.23 SE.seek 26.51 Pucks below Top Plate @23:56:54.39 Elbow.reconfigure :limp @23:56:54.46 Forearm.reconfigure :loose @23:56:54.51 Forearm.seek 4 @23:56:54.79 Elbow.hold @23:56:54.87 Elbow.reconfigure :tight @23:56:54.93 SE.reconfigure :default @23:56:54.98 SE.seek 26.69 Pucks below Top Plate @23:56:55.45 Forearm.force -20 @23:56:55.50 Forearm.seek 4 + 292 counts @23:56:55.78 Forearm.reconfigure :tight @23:56:55.84 Forearm.seek 4 + 450 counts @23:56:56.12 SE.seek :down @23:56:57.01 SE.stop @23:56:57.27 Forearm.reconfigure :loose @23:56:57.33 Forearm.seek :retract @23:56:58.44 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @23:56:58.86 Elbow.seek FlushPuck::Garage @23:57:00.92 Elbow.reconfigure :loose @23:57:01.13 Forearm.seek :garage @23:57:02.17 Hand.close @23:57:03.10 Forearm.seek :retract @23:57:04.16 Elbow.reconfigure :tight @23:57:04.26 Elbow.seek PC @23:57:05.62 Elbow.reconfigure :loose @23:57:05.89 Forearm.reconfigure :tight @23:57:06.00 Forearm.seek PC @23:57:07.78 PC.seek :closed @23:57:13.84 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.65 @23:57:13.98 Hand.open @23:57:14.81 Forearm.reconfigure :loose @23:57:14.90 Forearm.seek :retract @23:57:15.94 PSR.select :air @23:57:15.98 PSV.dial PSR @23:57:16.50 Pulling 0.5ml of PSR.flush @23:57:16.52 PSR.select :flush @23:57:16.71 PS.seek 0.5ml @23:57:19.50 CTV.dial :air,avoiding: :intake @23:57:20.91 Fix #1 of 2 (with RNAlater) for 2:30 @23:57:20.95 CSV.dial CSR @23:57:21.45 Pulling 2ml of CSR.RNAlater @23:57:21.47 CSR.select :RNAlater @23:57:21.66 CS.seek 2ml @23:57:22.78 delay 5 seconds @23:57:26.05 upsync @23:57:27.65 delay 5 seconds @23:57:27.80 PSR.select :air @23:57:27.91 PS.seek 2.5ml @23:57:32.67 CSR.select :air @23:57:32.79 CS.reconfigure :fast @23:57:32.84 CS.seek 10ml @23:57:49.20 delay 1 second @23:57:50.22 PRV.dial :waste2,avoiding: :ab2 @23:57:52.11 PTV.dial :PRVmixing @23:57:52.31 Pumping wcr puck from tube 5 up to 35psi... @23:57:52.33 CBV.dial SSV @23:57:53.44 PSV.dial :mixloop @23:57:53.58 CBV.dial between CSV and waste2b @23:57:54.53 PS.seek :empty @23:57:54.65 CSV.dial between air and CTV @23:57:55.25 CS.seek 6.945ml @23:58:03.00 CTV.dial CSV,avoiding: :air @23:58:03.02 CSV.dial CTV @23:58:04.42 CS.reconfigure :pressurize @23:58:04.48 CS.seek 0ml @23:58:20.95 PSR.select :air @23:58:20.97 PSV.dial PSR @23:58:22.02 Pulling 0.5ml of PSR.flush @23:58:22.03 PSR.select :flush @23:58:22.15 PS.seek 0.5ml @23:58:22.52 delay 2:30 @23:58:28.08 delay 5 seconds @23:58:33.10 PSR.select :air @23:58:33.22 PS.seek 2.5ml @23:58:54.52 delay 1 second @23:58:55.54 PBV.dial :waste1 @23:58:55.91 PTV.dial :mixing @23:58:56.67 PSV.dial :mixloop @23:58:57.85 PS.seek :empty @23:59:24.27 PSV.dial PSR @23:59:25.31 PSR.select :flush @23:59:25.44 PS.seek 1ml @23:59:36.54 PSR.select :air @23:59:36.67 PS.seek 2.5ml @23:59:52.76 PSV.dial PTV @23:59:53.66 PTV.dial :puck @23:59:54.39 PBV.dial :waste1 @23:59:54.47 PS.seek 0ml @00:00:21.01UTC07-Dec-23 PSV.dial PSR @00:00:21.76 PSR.select :flush @00:00:21.89 PS.seek 0.5ml @00:00:28.01 PSR.select :air @00:00:28.13 PS.seek 2.5ml @00:00:49.39 PSV.dial PTV @00:00:50.15 PBV.dial PSV @00:00:50.72 PS.seek 0ml @00:00:52.54 CBV.dial :waste2b @00:00:52.87 CS.reconfigure :default @00:00:52.92 CS.seek 0ml @00:00:54.24 delay 1:00 @00:01:17.26 PSV.dial PBV @00:01:17.74 PS.seek 2.5ml @00:01:44.14 PSV.dial :waste1 @00:01:44.88 PS.seek 0ml @00:01:54.27 CTV.dial :air,avoiding: :intake @00:01:55.61 Fix #2 of 2 (with RNAlater) for 2:30 @00:01:55.62 CSV.dial CSR @00:01:56.07 Pulling 2ml of CSR.RNAlater @00:01:56.08 CSR.select :RNAlater @00:01:56.20 CS.seek 2ml @00:02:02.12 delay 5 seconds @00:02:07.14 CSR.select :air @00:02:07.26 CS.reconfigure :fast @00:02:07.76 CS.seek 10ml @00:02:11.26 PSV.dial PSR @00:02:11.73 PSR.select :air @00:02:11.76 PS.seek 2.5ml @00:02:27.31 Pumping wcr puck from tube 5 up to 35psi... @00:02:27.32 CBV.dial SSV @00:02:28.58 CBV.dial between CSV and waste2b @00:02:29.55 CSV.dial between air and CTV @00:02:30.16 CS.seek 6.976ml @00:02:38.06 CTV.dial CSV,avoiding: :air @00:02:38.08 CSV.dial CTV @00:02:38.21 PSV.dial PTV @00:02:38.96 PBV.dial PSV @00:02:39.16 PS.seek 0ml @00:02:39.47 CS.reconfigure :pressurize @00:02:39.52 CS.seek 0ml @00:02:52.82 delay 2:30 @00:03:05.57 PSV.dial PBV @00:03:06.05 PS.seek 2.5ml @00:03:32.45 PSV.dial :waste1 @00:03:33.21 PS.seek 0ml @00:03:59.54 PSV.dial PSR @00:04:00.01 PSR.select :flush @00:04:00.14 PS.seek 1ml @00:04:11.23 PSR.select :air @00:04:11.35 PS.seek 2.5ml @00:04:27.42 PSV.dial PTV @00:04:28.15 PTV.dial :puck @00:04:28.23 PBV.dial :waste1 @00:04:28.78 PS.seek 0ml @00:04:55.35 PSV.dial PSR @00:04:56.10 PSR.select :flush @00:04:56.23 PS.seek 0.5ml @00:05:02.13 PSR.select :air @00:05:02.25 PS.seek 2.5ml @00:05:22.85 CBV.dial :waste2b @00:05:23.20 CS.reconfigure :default @00:05:23.25 CS.seek 0ml @00:05:23.57 PSV.dial PTV @00:05:24.35 PBV.dial PSV @00:05:24.92 PS.seek 0ml @00:05:28.99 delay 1:00 @00:05:51.35 PSV.dial PBV @00:05:51.83 PS.seek 2.5ml @00:06:18.23 PSV.dial :waste1 @00:06:18.97 PS.seek 0ml @00:06:29.02 CTV.dial :air,avoiding: :intake @00:06:30.39 Storing wcr puck in Collection Clamp to tube 4 @00:06:30.81 Elbow.reconfigure :tight @00:06:30.86 Elbow.seek 4 @00:06:33.48 Forearm.reconfigure :tight @00:06:33.54 Forearm.seek 4 + 450 counts @00:06:34.59 SE.reconfigure :pick @00:06:34.65 SE.seek :beamBreak @00:06:36.05 SE.configure :default @00:06:36.21 Elbow.reconfigure :limp @00:06:36.27 Forearm.reconfigure :loose @00:06:36.31 Forearm.seek 4 @00:06:36.56 Elbow.hold @00:06:36.63 Elbow.reconfigure :tight @00:06:36.73 SE.seek 27.1 Pucks below Top Plate @00:06:37.25 Forearm.force -20 @00:06:37.29 Forearm.seek 4 + 304 counts @00:06:37.70 Forearm.reconfigure :tight @00:06:37.75 Forearm.seek 4 + 450 counts @00:06:38.45 SE.seek 28.1 Pucks below Top Plate @00:06:39.02 Forearm.reconfigure :loose @00:06:39.07 Forearm.seek :retract @00:06:40.03 Elbow.seek CC @00:06:41.65 Elbow.reconfigure :loose @00:06:41.75 Forearm.seek CC @00:06:42.70 Hand.close @00:06:43.28 CC.seek :open @00:06:45.33 PSV.dial PSR @00:06:45.79 PSR.select :air @00:06:45.82 PS.seek 2.5ml @00:06:48.07 Forearm.seek :retract @00:06:49.10 Elbow.reconfigure :tight @00:06:49.15 Elbow.seek 4 @00:06:50.94 Elbow.reconfigure :limp @00:06:50.99 Forearm.seek 4 @00:06:51.98 Elbow.hold @00:06:52.05 Elbow.reconfigure :tight @00:06:52.11 Hand.open @00:06:52.90 Forearm.force -20 @00:06:52.95 Forearm.seek 4 + 317 counts @00:06:53.35 Forearm.reconfigure :tight @00:06:53.41 Forearm.seek 4 + 450 counts @00:06:53.83 SE.seek :down @00:06:54.63 SE.stop @00:06:55.35 Forearm.reconfigure :loose @00:06:55.47 Forearm.seek :retract @00:06:56.44 Starting finalPurge @00:07:12.16 PSV.dial PTV @00:07:12.90 PBV.dial PSV @00:07:13.12 PS.seek 0ml @00:07:15.95
Waiting >20 seconds for psFlush to relinquish FlushPuck @00:07:39.63 PSV.dial PBV @00:07:40.11 PS.seek 2.5ml @00:08:06.47 PSV.dial :waste1 @00:08:07.34 PS.seek 0ml @00:08:33.70
Loading FlushPuck into Collection Clamp from Processing Clamp @00:08:33.89 Elbow.seek PC @00:08:36.38 Elbow.reconfigure :loose @00:08:36.48 Forearm.seek PC @00:08:37.46 Hand.close @00:08:38.03 PC.seek :open @00:08:42.75 Forearm.seek :retract @00:08:43.72 Elbow.reconfigure :tight @00:08:43.76 Elbow.seek CC @00:08:47.41 Elbow.reconfigure :loose @00:08:47.51 Forearm.reconfigure :tight @00:08:47.56 Forearm.seek CC @00:08:48.76 CC.seek :closed @00:08:53.28 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.37 @00:08:53.34 Hand.open @00:08:53.96 Forearm.reconfigure :loose @00:08:54.01 Forearm.seek :retract @00:08:55.43 Flushing the Collection Valve::Manifold @00:08:55.56 CSV.dial CSR @00:08:56.00 Pulling 5ml of CSR.flush @00:08:56.01 CSR.select :flush @00:08:56.13 CS.seek 5ml @00:09:10.11 delay 5 seconds @00:09:15.13 CSR.select :air @00:09:15.30 CS.reconfigure :fast @00:09:15.35 CS.seek 7.499ml @00:09:22.11 CSV.dial :waste @00:09:23.40 CS.reconfigure :default @00:09:23.44 CS.seek 0ml @00:09:43.42 Flushing lower manifold's waste1 port @00:09:43.55 CSV.dial :flush @00:09:44.01 CS.reconfigure :fast @00:09:44.05 CS.seek 5ml @00:09:56.89 delay 5 seconds @00:10:01.91 CSV.dial :air @00:10:02.42 CS.seek 7.001ml @00:10:07.99 CSV.dial CTV @00:10:08.02
CTV.dial CSV @00:10:09.40 CBV.dial :waste1 @00:10:10.18 CS.seek 0ml @00:10:26.77 Flushing lower manifold's waste2 port @00:10:26.89 CSV.dial :flush @00:10:27.63 CS.seek 5ml @00:10:40.48 delay 5 seconds @00:10:45.50 CSV.dial :air @00:10:46.03 CS.seek 7ml @00:10:51.64 CSV.dial CTV @00:10:51.66
CTV.dial CSV @00:10:52.12 CBV.dial :waste2 @00:10:53.16 CS.seek 0ml @00:11:09.80 Flushing lower manifold's waste2b port @00:11:09.92 CSV.dial :flush @00:11:10.66 CS.seek 5ml @00:11:23.48 delay 5 seconds @00:11:28.50 CSV.dial :air @00:11:29.03 CS.seek 7ml @00:11:34.48 CSV.dial CTV @00:11:34.51
CTV.dial CSV @00:11:34.97 CBV.dial :waste2b @00:11:36.02 CS.seek 0ml @00:11:52.51 Disinfecting external Intake with kill @00:11:53.08 CSV.dial CSR @00:11:53.51 Pulling 8ml of CSR.kill @00:11:53.52 CSR.select :kill @00:11:53.64 CS.seek 8ml @00:12:13.33 delay 5 seconds @00:12:18.35 CSR.select :air @00:12:18.47 CS.seek 10ml @00:12:23.95 CSV.dial CBV @00:12:23.98
CBV.dial CSV @00:12:25.04 CTV.dial :intake @00:12:25.81 Intake.select :open @00:12:25.92 CS.seek 2ml @00:12:44.73 Intake.select :close @00:12:44.84 CTV.dial :air @00:12:45.59 CS.reconfigure :default @00:12:45.64 CS.seek 0ml @00:12:51.57 CSV.dial CSR @00:12:52.59 Pulling 5ml of CSR.flush @00:12:52.60 CSR.select :flush @00:12:52.72 CS.seek 5ml @00:13:06.57 delay 5 seconds @00:13:11.59 CSR.select :air @00:13:11.71 CS.seek 10ml @00:13:25.35 CSV.dial CBV @00:13:26.38 CS.seek 0ml @00:13:52.79 Garaging FlushPuck in Collection Clamp @00:13:52.86 Elbow.reconfigure :tight @00:13:52.91 Elbow.seek CC @00:13:53.24 Elbow.reconfigure :loose @00:13:53.34 Forearm.seek CC @00:13:54.38 Hand.close @00:13:55.03 CC.seek :open @00:13:59.66 Forearm.seek :retract @00:14:00.71 Elbow.reconfigure :tight @00:14:00.76 Elbow.seek :garage @00:14:03.67 Elbow.reconfigure :loose @00:14:03.77 Forearm.reconfigure :tight @00:14:03.82 Forearm.seek :garage @00:14:04.84 Hand.open @00:14:05.46 Forearm.reconfigure :loose @00:14:05.51 Forearm.seek :retract @00:14:06.49 Completed [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] begun 21:32:22 @00:14:06.55 Asleep after completing DA_WCR_120623b assays @00:14:06.57 GoodNews.email "{1 => 33,\n 2 => 33,\n 3 => 31,\n 4 => 29,\n 5 => 4,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@00:11:43, 30% flow, 16.9C, 32% humidity, 15.1psia, 13.710V, 0.423A, 165.950Ah, 5.80W\nSampled 650.0ml",Subject: "Asleep after completing DA_WCR_120623b assays" @00:14:11.50 upsync + ESPwhich DA_WCR_120723.rb + fn=DA_WCR_120723.rb + IFS=: + set /home/esp/esp2/mission . + fullPath=/home/esp/esp2/mission/DA_WCR_120723.rb + [ -f /home/esp/esp2/mission/DA_WCR_120723.rb ] + realpath /home/esp/esp2/mission/DA_WCR_120723.rb + return 0 + scriptPath=/home/esp/esp2local/mission/DA_WCR_120723.rb + exec ruby /home/esp/esp2/lib/esp.rb /home/esp/esp2local/mission/DA_WCR_120723.rb @13:10:43.27UTC07-Dec-23
ESP2 v3.42 I2C Gateway v3.20 for core bus @13:10:45.89 Configuration for short puck jake 1/11/21 by brent@mbari.org /home/esp/esp2/type/shallow/jake/configure.rb @13:10:49.50 Polling Can every 10:00 @13:10:49.59 Cleanly exited at 13:07:45UTC07-Dec-23 @13:10:49.64 Loading /home/esp/esp2local/mission/DA_WCR_120723.rb mission @13:10:49.70 Preparing to sleep until + 00:00:05 @13:10:49.71 GoodNews.email "Starting DA_WCR_120723 w/pucks in tube 5\n{1 => 33,\n 2 => 33,\n 3 => 31,\n 4 => 29,\n 5 => 4,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@13:10:49, 31% flow, 16.0C, 31% humidity, 15.1psia, 13.754V, 0.248A, 168.992Ah, 3.41W",Subject: "DA_WCR_120723 begun" @13:10:49.76
Asleep til + 00:00:05 @13:10:49.85 Continuing at 13:10 @13:10:50.03 Gate.power :core,:OFF @13:10:50.05 Gate.power :raw,:OFF @13:10:50.06 Shutdown @13:10:53.07 GoodNews.email "Then, in tube 5: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_120723.rb:14\n{1 => 33,\n 2 => 33,\n 3 => 31,\n 4 => 29,\n 5 => 4,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@13:10:49, 31% flow, 16.0C, 31% humidity, 15.1psia, 13.754V, 0.242A, 168.992Ah, 3.33W",Subject: "Asleep until 13:10" @13:10:54.46 upsync @13:10:54.69
Powering core bus @13:10:57.63 upsync @13:11:00.10
Gate.power :raw,:ON @13:11:01.06 CS.configure :default @13:11:01.11 PS.configure :default @13:11:01.16 SS.configure :default @13:11:01.21 CC.configure :default @13:11:01.26 PC.configure :default @13:11:01.32 Forearm.configure :loose @13:11:01.37 Elbow.configure :loose @13:11:01.44 SC.configure :loose @13:11:01.49 SE.configure :default @13:11:01.54 CH.configure :default @13:11:01.57 PH.configure :default @13:11:01.61 All dwarf controllers running firmware version 3.85 @13:11:01.72 Valve.reset @13:11:01.93 Intake.select :close @13:11:01.99 Exhaust.select :close @13:11:02.03 CTV.dial :air @13:11:02.14 PSV.dial :air @13:11:02.18 CSV.dial :waste1 @13:11:02.29
Forearm.home.seek :retract @13:11:02.65 SSV.dial :spit,avoiding: :exhaust @13:11:03.05 Hand.close @13:11:03.09
Elbow.reconfigure :loose @13:11:03.17 Elbow.jog 1500 @13:11:03.40 PS.home.seek 0ml @13:11:03.53 CS.reconfigure :fast @13:11:03.56 SS.home.seek 0ml @13:11:03.67 CS.home.seek 0ml @13:11:03.73
Elbow.reconfigure :loose @13:11:03.78 Elbow.home.seek :clear @13:11:07.13 SE.home.seek :down @13:11:07.73 SC.reconfigure :tight @13:11:07.78 SC.home.seek :initial @13:11:11.56 PS.stop @13:11:26.72
SC.configure :limp @13:11:26.77 Forearm.seek :retract @13:11:26.84 Elbow.seek :hammer @13:11:27.90 Forearm.reconfigure :tight @13:11:27.95 Forearm.seek retracted @13:11:28.51 CC.home.seek :open @13:11:28.59 PC.home.seek :open @13:11:32.00
Phase [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] @13:11:32.02 Notice.email "At 13:11:31 began in tube 5: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_120723.rb:14\n{1 => 33,\n 2 => 33,\n 3 => 31,\n 4 => 29,\n 5 => 4,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@13:11:31, 31% flow, 16.1C, 31% humidity, 15.1psia, 13.676V, 0.296A, 168.996Ah, 4.05W",Subject: "Started DA_WCR_120723 phase initialPurge; da [1000, 1000] #da and wsr are run simultanelously" @13:11:32.05
Starting initialPurge @13:11:32.06 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @13:11:32.13 Hand.open @13:11:32.88 Elbow.reconfigure :tight @13:11:32.93 Elbow.seek FlushPuck::Garage @13:11:34.74 Elbow.reconfigure :loose @13:11:34.84 Forearm.reconfigure :loose @13:11:34.89 Forearm.seek :garage @13:11:35.83 Hand.close @13:11:36.64 Forearm.seek :retract @13:11:37.30 upsync @13:11:37.65
Elbow.reconfigure :tight @13:11:37.70 Elbow.seek CC @13:11:40.71 Elbow.reconfigure :loose @13:11:40.81 Forearm.reconfigure :tight @13:11:40.86 Forearm.seek CC @13:11:42.15 CC.seek :closed @13:11:46.68 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.47 @13:11:46.75 Hand.open @13:11:47.45 Forearm.reconfigure :loose @13:11:47.50 Forearm.seek :retract @13:11:48.45 Flushing the Collection Valve::Manifold @13:11:48.57 CSV.dial CSR @13:11:49.86 Pulling 5ml of CSR.flush @13:11:49.87 CSR.select :flush @13:11:49.99 CS.reconfigure :default @13:11:50.03 CS.seek 5ml @13:12:04.43 delay 5 seconds @13:12:09.44 CSR.select :air @13:12:09.61 CS.reconfigure :fast @13:12:09.66 CS.seek 7.499ml @13:12:16.49 CSV.dial :waste @13:12:17.79 CS.reconfigure :default @13:12:17.84 CS.seek 0ml @13:12:37.90 Purging external Intake @13:12:38.02 CSV.dial :flush @13:12:38.48 CS.reconfigure :fast @13:12:38.53 CS.seek 10ml @13:13:03.02 delay 5 seconds @13:13:08.04 CSV.dial CBV @13:13:08.07
CBV.dial CSV @13:13:08.83 CTV.dial :intake @13:13:09.63 Intake.select :open @13:13:09.74 CS.seek 0ml @13:13:33.20 Intake.select :close @13:13:33.31 CTV.dial :air @13:13:34.10 Flushing upper manifold @13:13:34.21 CSV.dial :flush @13:13:34.95 CS.seek 5ml @13:13:47.83 delay 5 seconds @13:13:52.85 CSV.dial :air @13:13:53.36 CS.seek 7ml @13:13:58.96 CSV.dial CBV @13:13:58.98
CBV.dial CSV @13:14:00.03 CTV.dial :air @13:14:00.12 CS.seek 0ml @13:14:16.77 delay 7 seconds @13:14:23.79 CS.seek 3ml @13:14:31.89 CSV.dial :waste @13:14:32.35 CS.seek 0ml @13:14:40.12 CSV.dial :flush @13:14:40.69 CS.seek 3ml @13:14:48.73 delay 5 seconds @13:14:53.75 CSV.dial :air @13:14:54.28 CS.seek 5ml @13:14:59.83 CSV.dial :air @13:15:00.01 CS.seek 0ml @13:15:12.23 delay 7 seconds @13:15:19.26 CS.seek 3ml @13:15:27.33 CSV.dial :waste @13:15:28.07 CS.seek 0ml @13:15:35.83 Garaging FlushPuck in Collection Clamp @13:15:35.90 Elbow.reconfigure :tight @13:15:35.95 Elbow.seek CC @13:15:36.28 Elbow.reconfigure :loose @13:15:36.38 Forearm.seek CC @13:15:37.42 Hand.close @13:15:38.01 CC.seek :open @13:15:43.03 Forearm.seek :retract @13:15:44.04 Elbow.reconfigure :tight @13:15:44.09 Elbow.seek :garage @13:15:47.03 Elbow.reconfigure :loose @13:15:47.13 Forearm.reconfigure :tight @13:15:47.18 Forearm.seek :garage @13:15:48.20 Hand.open @13:15:48.81 Forearm.reconfigure :loose @13:15:48.86 Forearm.seek :retract @13:15:49.85 da sampling at most 1000ml, wcr at most 1000ml @13:15:49.89 Loading sh1 puck from tube 5 into Collection Clamp @13:15:49.90 DAfans.email "Started da in tube 5 at 13:15:49UTC07-Dec-23\n{1 => 33,\n 2 => 33,\n 3 => 31,\n 4 => 29,\n 5 => 4,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@13:11:31, 31% flow, 16.1C, 31% humidity, 15.1psia, 13.676V, 0.296A, 168.996Ah, 4.05W",Subject: "da sampling at most 1000ml, wcr at most 1000ml" @13:15:50.60
SC.reconfigure :tight @13:15:50.66 SC.seek 5 @13:15:54.24 SC.configure :limp @13:15:54.31 upsync @13:15:54.50
Elbow.reconfigure :tight @13:15:54.55 Elbow.seek 5 @13:15:56.59 Forearm.reconfigure :tight @13:15:56.64 Forearm.seek 5 + 450 counts @13:15:57.68 SE.reconfigure :pick @13:15:57.73 SE.seek :beamBreak @13:16:00.75 SE.configure :default @13:16:00.92 Elbow.seek 5 @13:16:01.11 Forearm.seek 5 + 300 counts @13:16:01.36 Forearm.reconfigure :loose @13:16:01.41 Hand.close @13:16:02.02 SE.seek :down @13:16:02.22
Forearm.seek :retract @13:16:03.60 Elbow.seek CC @13:16:04.59 SE.stop @13:16:05.25
Elbow.reconfigure :loose @13:16:05.35 Forearm.reconfigure :tight @13:16:05.40 Forearm.seek CC @13:16:06.60 CC.seek :closed @13:16:11.15 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.31 @13:16:11.16 Hand.open @13:16:11.81 Forearm.reconfigure :loose @13:16:11.86 Forearm.seek :retract @13:16:12.80 collectSample 1000ml w/bubble point of 24psi @13:16:12.81 Priming 7ml @13:16:12.82 Intake.select :close @13:16:12.83 Exhaust.select :close @13:16:12.85 SS.hold @13:16:13.05 Calibrating pressure and temperature sensors @13:16:13.08 CTV.dial :air @13:16:13.10 SSV.dial :spit,avoiding: :exhaust @13:16:13.30 SS.reconfigure :fast @13:16:13.35 SS.seek 5ml @13:16:16.16 SSV.dial CBV @13:16:16.18
CBV.dial SSV @13:16:17.53 delay 3.8 seconds @13:16:21.46 Sensor limits: Intake max 101.4psia; Outlet max 93.8psia SSV.dial :spit @13:16:22.78 SS.seek 0ml @13:16:25.56 SSV.dial CBV @13:16:26.88 CTV.dial :intake @13:16:27.66 SSV.dial CBV @13:16:27.68
CBV.dial SSV @13:16:27.82 Allowing 40s for 7.0ml of remaining 7.0ml @13:16:27.83 Intake.select :open @13:16:30.00 Intake @14.5psia, vacuum from 9.0 to 12.0psi @13:16:30.01 SS.reconfigure :inhaling 2.20ml/s @13:16:30.06 SS.seek 7ml @13:16:37.08 Stroke completed -- waiting for pressure to equalize ... @13:16:37.09 SS.reconfigure :exhale @13:16:37.15 SS.hold @13:16:42.96 Intake.select :close @13:16:43.12 SSV.dial :exhaust @13:16:43.87 Exhaust.select :open @13:16:43.98 SS.seek 0ml @13:16:47.90 Exhaust.select :close @13:16:48.03 Sampling 1000.0ml @13:16:48.05 Intake.select :close @13:16:48.07 Exhaust.select :close @13:16:48.10 SS.reconfigure :default @13:16:48.20 SS.hold @13:16:48.41 CTV.dial :intake @13:16:48.49 SSV.dial CBV @13:16:48.53
CBV.dial SSV @13:16:49.29 Allowing 130s for 25.0ml of remaining 1000.0ml @13:16:49.30 Intake.select :open @13:16:51.47 Intake @15.4psia, vacuum from 9.9 to 12.9psi @13:16:51.48 SS.reconfigure :inhaling 2.20ml/s @13:16:51.53 SS.seek 25ml @13:17:03.95 Vacuum too high -- waiting up to 40 seconds @13:17:03.97 SS.reconfigure :exhale @13:17:04.02 SS.hold @13:17:10.01 Intake @19.2psia, vacuum from 10.0 to 13.0psi @13:17:10.02 SS.reconfigure :inhaling 1.76ml/s @13:17:10.07 SS.seek 25ml @13:17:13.24 Vacuum too high -- waiting up to 40 seconds @13:17:13.25 SS.reconfigure :exhale @13:17:13.30 SS.hold @13:17:14.46 Intake @18.0psia, vacuum from 10.0 to 13.0psi @13:17:14.47 SS.reconfigure :inhaling 1.41ml/s @13:17:14.52 SS.seek 25ml @13:17:15.40 Stroke completed -- waiting for pressure to equalize ... @13:17:15.41 SS.reconfigure :exhale @13:17:15.45 SS.hold @13:17:41.88 Intake.select :close @13:17:42.03 SSV.dial :exhaust @13:17:42.80 Exhaust.select :open @13:17:42.91 SS.seek 0ml @13:17:51.87 Exhaust.select :close @13:17:52.40 SSV.dial CBV @13:17:52.43
CBV.dial SSV @13:17:53.21 Allowing 130s for 25.0ml of remaining 975.0ml @13:17:53.22 Intake.select :open @13:17:55.39 Intake @15.7psia, vacuum from 10.0 to 13.0psi @13:17:55.41 SS.reconfigure :inhaling 1.41ml/s @13:17:55.45 SS.seek 25ml @13:18:15.86 Stroke completed -- waiting for pressure to equalize ... @13:18:15.87 SS.reconfigure :exhale @13:18:15.92 SS.hold @13:18:46.54 Intake.select :close @13:18:46.69 SSV.dial :exhaust @13:18:47.44 Exhaust.select :open @13:18:47.98 SS.seek 0ml @13:18:56.77 Exhaust.select :close @13:18:56.88 SSV.dial CBV @13:18:56.90
CBV.dial SSV @13:18:57.71 Allowing 130s for 25.0ml of remaining 950.0ml @13:18:57.72 Intake.select :open @13:18:59.89 Intake @15.8psia, vacuum from 10.0 to 13.0psi @13:18:59.90 SS.reconfigure :inhaling 1.41ml/s @13:18:59.95 SS.seek 25ml @13:19:20.71 Stroke completed -- waiting for pressure to equalize ... @13:19:20.72 SS.reconfigure :exhale @13:19:20.77 SS.hold @13:19:49.24 Intake.select :close @13:19:49.39 SSV.dial :exhaust @13:19:50.15 Exhaust.select :open @13:19:50.69 SS.seek 0ml @13:19:59.44 Exhaust.select :close @13:19:59.55 SSV.dial CBV @13:19:59.57
CBV.dial SSV @13:20:00.38 Allowing 130s for 25.0ml of remaining 925.0ml @13:20:00.39 Intake.select :open @13:20:02.56 Intake @15.8psia, vacuum from 10.0 to 13.0psi @13:20:02.57 SS.reconfigure :inhaling 1.41ml/s @13:20:02.62 SS.seek 25ml @13:20:23.16 Stroke completed -- waiting for pressure to equalize ... @13:20:23.17 SS.reconfigure :exhale @13:20:23.22 SS.hold @13:20:49.77 Intake.select :close @13:20:49.92 SSV.dial :exhaust @13:20:50.68 Exhaust.select :open @13:20:50.80 SS.seek 0ml @13:21:00.00 Exhaust.select :close @13:21:00.11 SSV.dial CBV @13:21:00.14
CBV.dial SSV @13:21:00.91 Allowing 130s for 25.0ml of remaining 900.0ml @13:21:00.92 Intake.select :open @13:21:03.09 Intake @15.9psia, vacuum from 10.0 to 13.0psi @13:21:03.10 SS.reconfigure :inhaling 1.41ml/s @13:21:03.15 SS.seek 25ml @13:21:23.69 Stroke completed -- waiting for pressure to equalize ... @13:21:23.70 SS.reconfigure :exhale @13:21:23.75 SS.hold @13:21:46.16 Intake.select :close @13:21:46.31 SSV.dial :exhaust @13:21:47.08 Exhaust.select :open @13:21:47.20 SS.seek 0ml @13:21:55.92 Exhaust.select :close @13:21:56.03 SSV.dial CBV @13:21:56.06
CBV.dial SSV @13:21:56.83 Allowing 130s for 25.0ml of remaining 875.0ml @13:21:56.84 Intake.select :open @13:21:59.43 Intake @16.0psia, vacuum from 10.0 to 13.0psi @13:21:59.45 SS.reconfigure :inhaling 1.41ml/s @13:21:59.50 SS.seek 25ml @13:22:19.94 Stroke completed -- waiting for pressure to equalize ... @13:22:19.95 SS.reconfigure :exhale @13:22:20.00 SS.hold @13:22:40.32 Intake.select :close @13:22:40.47 SSV.dial :exhaust @13:22:41.24 Exhaust.select :open @13:22:41.36 SS.seek 0ml @13:22:50.06 Exhaust.select :close @13:22:50.17 SSV.dial CBV @13:22:50.19
CBV.dial SSV @13:22:51.00 Allowing 130s for 25.0ml of remaining 850.0ml @13:22:51.01 Intake.select :open @13:22:53.18 Intake @16.0psia, vacuum from 10.0 to 13.0psi @13:22:53.19 SS.reconfigure :inhaling 1.41ml/s @13:22:53.24 SS.seek 25ml @13:23:13.73 Stroke completed -- waiting for pressure to equalize ... @13:23:13.74 SS.reconfigure :exhale @13:23:13.79 SS.hold @13:23:34.43 Intake.select :close @13:23:34.59 SSV.dial :exhaust @13:23:35.35 Exhaust.select :open @13:23:35.47 SS.seek 0ml @13:23:44.23 Exhaust.select :close @13:23:44.34 SSV.dial CBV @13:23:44.36
CBV.dial SSV @13:23:45.14 Allowing 130s for 25.0ml of remaining 825.0ml @13:23:45.15 Intake.select :open @13:23:47.32 Intake @16.0psia, vacuum from 10.0 to 13.0psi @13:23:47.33 SS.reconfigure :inhaling 1.41ml/s @13:23:47.38 SS.seek 25ml @13:24:08.15 Stroke completed -- waiting for pressure to equalize ... @13:24:08.16 SS.reconfigure :exhale @13:24:08.21 SS.hold @13:24:29.06 Intake.select :close @13:24:29.21 SSV.dial :exhaust @13:24:29.98 Exhaust.select :open @13:24:30.09 SS.seek 0ml @13:24:38.80 Exhaust.select :close @13:24:38.91 SSV.dial CBV @13:24:38.93
CBV.dial SSV @13:24:39.72 Allowing 130s for 25.0ml of remaining 800.0ml @13:24:39.73 Intake.select :open @13:24:41.90 Intake @15.8psia, vacuum from 10.0 to 13.0psi @13:24:41.91 SS.reconfigure :inhaling 1.41ml/s @13:24:41.96 SS.seek 25ml @13:25:02.45 Stroke completed -- waiting for pressure to equalize ... @13:25:02.46 SS.reconfigure :exhale @13:25:02.51 SS.hold @13:25:20.86 Intake.select :close @13:25:21.01 SSV.dial :exhaust @13:25:21.78 Exhaust.select :open @13:25:21.89 SS.seek 0ml @13:25:30.61 Exhaust.select :close @13:25:30.71 SSV.dial CBV @13:25:30.73
CBV.dial SSV @13:25:31.53 Allowing 130s for 25.0ml of remaining 775.0ml @13:25:31.54 Intake.select :open @13:25:33.72 Intake @15.9psia, vacuum from 10.0 to 13.0psi @13:25:33.73 SS.reconfigure :inhaling 1.41ml/s @13:25:33.78 SS.seek 25ml @13:25:54.21 Stroke completed -- waiting for pressure to equalize ... @13:25:54.22 SS.reconfigure :exhale @13:25:54.27 SS.hold @13:26:10.98 Intake.select :close @13:26:11.14 SSV.dial :exhaust @13:26:11.92 Exhaust.select :open @13:26:12.03 SS.seek 0ml @13:26:20.75 Exhaust.select :close @13:26:20.85 SSV.dial CBV @13:26:20.88
CBV.dial SSV @13:26:21.67 Allowing 130s for 25.0ml of remaining 750.0ml @13:26:21.68 Intake.select :open @13:26:23.85 Intake @16.0psia, vacuum from 10.0 to 13.0psi @13:26:23.86 SS.reconfigure :inhaling 1.41ml/s @13:26:23.91 SS.seek 25ml @13:26:44.37 Stroke completed -- waiting for pressure to equalize ... @13:26:44.38 SS.reconfigure :exhale @13:26:44.43 SS.hold @13:27:00.73 Intake.select :close @13:27:00.88 SSV.dial :exhaust @13:27:01.64 Exhaust.select :open @13:27:01.75 SS.seek 0ml @13:27:10.54 Exhaust.select :close @13:27:10.66 SSV.dial CBV @13:27:10.68
CBV.dial SSV @13:27:11.47 Allowing 130s for 25.0ml of remaining 725.0ml @13:27:11.48 Intake.select :open @13:27:13.64 Intake @16.0psia, vacuum from 10.0 to 13.0psi @13:27:13.66 SS.reconfigure :inhaling 1.41ml/s @13:27:13.70 SS.seek 25ml @13:27:34.42 Stroke completed -- waiting for pressure to equalize ... @13:27:34.43 SS.reconfigure :exhale @13:27:34.47 SS.hold @13:27:48.85 Intake.select :close @13:27:49.01 SSV.dial :exhaust @13:27:49.76 Exhaust.select :open @13:27:49.88 SS.seek 0ml @13:27:58.59 Exhaust.select :close @13:27:58.69 SSV.dial CBV @13:27:58.72
CBV.dial SSV @13:27:59.50 Allowing 130s for 25.0ml of remaining 700.0ml @13:27:59.86 Intake.select :open @13:28:02.10 Intake @16.1psia, vacuum from 10.0 to 13.0psi @13:28:02.11 SS.reconfigure :inhaling 1.41ml/s @13:28:02.16 SS.seek 25ml @13:28:22.54 Stroke completed -- waiting for pressure to equalize ... @13:28:22.55 SS.reconfigure :exhale @13:28:22.60 SS.hold @13:28:38.87 Intake.select :close @13:28:39.03 SSV.dial :exhaust @13:28:39.79 Exhaust.select :open @13:28:39.91 SS.seek 0ml @13:28:48.60 Exhaust.select :close @13:28:48.72 SSV.dial CBV @13:28:48.76
CBV.dial SSV @13:28:49.49 Allowing 130s for 25.0ml of remaining 675.0ml @13:28:49.50 Intake.select :open @13:28:51.67 Intake @16.1psia, vacuum from 10.0 to 13.0psi @13:28:51.68 SS.reconfigure :inhaling 1.41ml/s @13:28:51.73 SS.seek 25ml @13:29:12.35 Stroke completed -- waiting for pressure to equalize ... @13:29:12.36 SS.reconfigure :exhale @13:29:12.41 SS.hold @13:29:28.80 Intake.select :close @13:29:28.96 SSV.dial :exhaust @13:29:29.71 Exhaust.select :open @13:29:29.83 SS.seek 0ml @13:29:38.45 Exhaust.select :close @13:29:38.55 SSV.dial CBV @13:29:38.59
CBV.dial SSV @13:29:39.34 Allowing 130s for 25.0ml of remaining 650.0ml @13:29:39.35 Intake.select :open @13:29:41.51 Intake @16.0psia, vacuum from 10.0 to 13.0psi @13:29:41.52 SS.reconfigure :inhaling 1.41ml/s @13:29:41.57 SS.seek 25ml @13:30:02.13 Stroke completed -- waiting for pressure to equalize ... @13:30:02.14 SS.reconfigure :exhale @13:30:02.19 SS.hold @13:30:18.63 Intake.select :close @13:30:18.78 SSV.dial :exhaust @13:30:19.54 Exhaust.select :open @13:30:19.65 SS.seek 0ml @13:30:28.37 Exhaust.select :close @13:30:28.47 SSV.dial CBV @13:30:28.49
CBV.dial SSV @13:30:29.27 Allowing 130s for 25.0ml of remaining 625.0ml @13:30:29.28 Intake.select :open @13:30:31.45 Intake @15.9psia, vacuum from 10.0 to 13.0psi @13:30:31.46 SS.reconfigure :inhaling 1.41ml/s @13:30:31.51 SS.seek 25ml @13:30:52.07 Stroke completed -- waiting for pressure to equalize ... @13:30:52.08 SS.reconfigure :exhale @13:30:52.13 SS.hold @13:31:08.32 Intake.select :close @13:31:08.48 SSV.dial :exhaust @13:31:09.24 Exhaust.select :open @13:31:09.36 SS.seek 0ml @13:31:18.32 Exhaust.select :close @13:31:18.43 SSV.dial CBV @13:31:18.45
CBV.dial SSV @13:31:19.21 Allowing 130s for 25.0ml of remaining 600.0ml @13:31:19.22 Intake.select :open @13:31:21.39 Intake @16.0psia, vacuum from 10.0 to 13.0psi @13:31:21.40 SS.reconfigure :inhaling 1.41ml/s @13:31:21.45 SS.seek 25ml @13:31:41.54 Stroke completed -- waiting for pressure to equalize ... @13:31:41.55 SS.reconfigure :exhale @13:31:41.59 SS.hold @13:31:55.96 Intake.select :close @13:31:56.53 SSV.dial :exhaust @13:31:57.30 Exhaust.select :open @13:31:57.42 SS.seek 0ml @13:32:06.13 Exhaust.select :close @13:32:06.24 SSV.dial CBV @13:32:06.26
CBV.dial SSV @13:32:07.05 Allowing 130s for 25.0ml of remaining 575.0ml @13:32:07.06 Intake.select :open @13:32:09.23 Intake @16.1psia, vacuum from 10.0 to 13.0psi @13:32:09.24 SS.reconfigure :inhaling 1.41ml/s @13:32:09.29 SS.seek 25ml @13:32:29.97 Stroke completed -- waiting for pressure to equalize ... @13:32:29.98 SS.reconfigure :exhale @13:32:30.03 SS.hold @13:32:46.32 Intake.select :close @13:32:46.47 SSV.dial :exhaust @13:32:47.22 Exhaust.select :open @13:32:47.34 SS.seek 0ml @13:32:56.11 Exhaust.select :close @13:32:56.23 SSV.dial CBV @13:32:56.26
CBV.dial SSV @13:32:57.00 Allowing 130s for 25.0ml of remaining 550.0ml @13:32:57.02 Intake.select :open @13:32:59.18 Intake @16.0psia, vacuum from 10.0 to 13.0psi @13:32:59.55 SS.reconfigure :inhaling 1.41ml/s @13:32:59.67 SS.seek 25ml @13:33:20.32 Stroke completed -- waiting for pressure to equalize ... @13:33:20.33 SS.reconfigure :exhale @13:33:20.37 SS.hold @13:33:36.57 Intake.select :close @13:33:36.73 SSV.dial :exhaust @13:33:37.49 Exhaust.select :open @13:33:37.60 SS.seek 0ml @13:33:46.53 Exhaust.select :close @13:33:46.64 SSV.dial CBV @13:33:46.68
CBV.dial SSV @13:33:47.43 Allowing 130s for 25.0ml of remaining 525.0ml @13:33:47.44 Intake.select :open @13:33:49.60 Intake @16.0psia, vacuum from 10.0 to 13.0psi @13:33:49.61 SS.reconfigure :inhaling 1.41ml/s @13:33:49.66 SS.seek 25ml @13:34:09.69 Stroke completed -- waiting for pressure to equalize ... @13:34:09.70 SS.reconfigure :exhale @13:34:09.75 SS.hold @13:34:26.36 Intake.select :close @13:34:26.52 SSV.dial :exhaust @13:34:27.28 Exhaust.select :open @13:34:27.40 SS.seek 0ml @13:34:36.13 Exhaust.select :close @13:34:36.23 SSV.dial CBV @13:34:36.25
CBV.dial SSV @13:34:37.03 Allowing 130s for 25.0ml of remaining 500.0ml @13:34:37.04 Intake.select :open @13:34:39.21 Intake @16.1psia, vacuum from 10.0 to 13.0psi @13:34:39.23 SS.reconfigure :inhaling 1.41ml/s @13:34:39.27 SS.seek 25ml @13:34:59.70 Stroke completed -- waiting for pressure to equalize ... @13:34:59.71 SS.reconfigure :exhale @13:34:59.76 SS.hold @13:35:16.47 Intake.select :close @13:35:16.62 SSV.dial :exhaust @13:35:17.38 Exhaust.select :open @13:35:17.49 SS.seek 0ml @13:35:26.24 Exhaust.select :close @13:35:26.34 SSV.dial CBV @13:35:26.36
CBV.dial SSV @13:35:27.17 Allowing 130s for 25.0ml of remaining 475.0ml @13:35:27.19 Intake.select :open @13:35:29.35 Intake @16.0psia, vacuum from 10.0 to 13.0psi @13:35:29.36 SS.reconfigure :inhaling 1.41ml/s @13:35:29.41 SS.seek 25ml @13:35:49.86 Stroke completed -- waiting for pressure to equalize ... @13:35:49.87 SS.reconfigure :exhale @13:35:49.92 SS.hold @13:36:06.12 Intake.select :close @13:36:06.27 SSV.dial :exhaust @13:36:07.05 Exhaust.select :open @13:36:07.16 SS.seek 0ml @13:36:15.86 Exhaust.select :close @13:36:15.97 SSV.dial CBV @13:36:15.99
CBV.dial SSV @13:36:16.78 Allowing 130s for 25.0ml of remaining 450.0ml @13:36:16.79 Intake.select :open @13:36:18.96 Intake @16.2psia, vacuum from 10.0 to 13.0psi @13:36:18.97 SS.reconfigure :inhaling 1.41ml/s @13:36:19.02 SS.seek 25ml @13:36:39.59 Stroke completed -- waiting for pressure to equalize ... @13:36:39.60 SS.reconfigure :exhale @13:36:39.65 SS.hold @13:36:56.82 Intake.select :close @13:36:56.98 SSV.dial :exhaust @13:36:57.74 Exhaust.select :open @13:36:57.85 SS.seek 0ml @13:37:06.77 Exhaust.select :close @13:37:06.87 SSV.dial CBV @13:37:06.89
CBV.dial SSV @13:37:07.69 Allowing 130s for 25.0ml of remaining 425.0ml @13:37:07.70 Intake.select :open @13:37:09.87 Intake @16.2psia, vacuum from 10.0 to 13.0psi @13:37:09.88 SS.reconfigure :inhaling 1.41ml/s @13:37:09.93 SS.seek 25ml @13:37:29.95 Stroke completed -- waiting for pressure to equalize ... @13:37:29.96 SS.reconfigure :exhale @13:37:30.01 SS.hold @13:37:48.77 Intake.select :close @13:37:48.92 SSV.dial :exhaust @13:37:49.69 Exhaust.select :open @13:37:49.80 SS.seek 0ml @13:37:58.73 Exhaust.select :close @13:37:58.84 SSV.dial CBV @13:37:58.87
CBV.dial SSV @13:37:59.64 Allowing 130s for 25.0ml of remaining 400.0ml @13:37:59.65 Intake.select :open @13:38:01.82 Intake @16.0psia, vacuum from 10.0 to 13.0psi @13:38:01.83 SS.reconfigure :inhaling 1.41ml/s @13:38:01.88 SS.seek 25ml @13:38:21.86 Stroke completed -- waiting for pressure to equalize ... @13:38:21.87 SS.reconfigure :exhale @13:38:21.91 SS.hold @13:38:40.21 Intake.select :close @13:38:40.36 SSV.dial :exhaust @13:38:41.54 Exhaust.select :open @13:38:41.66 SS.seek 0ml @13:38:50.40 Exhaust.select :close @13:38:50.52 SSV.dial CBV @13:38:50.56
CBV.dial SSV @13:38:51.30 Allowing 130s for 25.0ml of remaining 375.0ml @13:38:51.31 Intake.select :open @13:38:53.47 Intake @16.1psia, vacuum from 10.0 to 13.0psi @13:38:53.48 SS.reconfigure :inhaling 1.41ml/s @13:38:53.53 SS.seek 25ml @13:39:13.93 Stroke completed -- waiting for pressure to equalize ... @13:39:13.94 SS.reconfigure :exhale @13:39:13.99 SS.hold @13:39:32.32 Intake.select :close @13:39:32.48 SSV.dial :exhaust @13:39:33.23 Exhaust.select :open @13:39:33.35 SS.seek 0ml @13:39:42.12 Exhaust.select :close @13:39:42.23 SSV.dial CBV @13:39:42.25
CBV.dial SSV @13:39:43.11 Allowing 130s for 25.0ml of remaining 350.0ml @13:39:43.12 Intake.select :open @13:39:45.29 Intake @16.2psia, vacuum from 10.0 to 13.0psi @13:39:45.31 SS.reconfigure :inhaling 1.41ml/s @13:39:45.35 SS.seek 25ml @13:40:03.49 Vacuum too high -- waiting up to 40 seconds @13:40:03.50 SS.reconfigure :exhale @13:40:03.56 SS.hold @13:40:11.21 Intake @17.5psia, vacuum from 10.0 to 13.0psi @13:40:11.23 SS.reconfigure :inhaling 1.13ml/s @13:40:11.27 SS.seek 25ml @13:40:14.37 Stroke completed -- waiting for pressure to equalize ... @13:40:14.38 SS.reconfigure :exhale @13:40:14.43 SS.hold @13:40:25.53 Intake.select :close @13:40:25.69 SSV.dial :exhaust @13:40:26.47 Exhaust.select :open @13:40:26.58 SS.seek 0ml @13:40:35.28 Exhaust.select :close @13:40:35.38 SSV.dial CBV @13:40:35.40
CBV.dial SSV @13:40:36.20 Allowing 130s for 25.0ml of remaining 325.0ml @13:40:36.21 Intake.select :open @13:40:38.38 Intake @16.2psia, vacuum from 10.0 to 13.0psi @13:40:38.39 SS.reconfigure :inhaling 1.13ml/s @13:40:38.44 SS.seek 25ml @13:41:02.92 Stroke completed -- waiting for pressure to equalize ... @13:41:02.93 SS.reconfigure :exhale @13:41:02.97 SS.hold @13:41:20.52 Intake.select :close @13:41:20.68 SSV.dial :exhaust @13:41:21.43 Exhaust.select :open @13:41:21.55 SS.seek 0ml @13:41:30.51 Exhaust.select :close @13:41:30.62 SSV.dial CBV @13:41:30.65
CBV.dial SSV @13:41:31.42 Allowing 130s for 25.0ml of remaining 300.0ml @13:41:31.43 Intake.select :open @13:41:33.59 Intake @16.2psia, vacuum from 10.0 to 13.0psi @13:41:33.60 SS.reconfigure :inhaling 1.13ml/s @13:41:33.65 SS.seek 25ml @13:41:57.85 Stroke completed -- waiting for pressure to equalize ... @13:41:57.86 SS.reconfigure :exhale @13:41:57.91 SS.hold @13:42:16.29 Intake.select :close @13:42:16.44 SSV.dial :exhaust @13:42:17.20 Exhaust.select :open @13:42:17.31 SS.seek 0ml @13:42:26.46 Exhaust.select :close @13:42:26.57 SSV.dial CBV @13:42:26.60
CBV.dial SSV @13:42:27.37 Allowing 130s for 25.0ml of remaining 275.0ml @13:42:27.38 Intake.select :open @13:42:29.55 Intake @16.2psia, vacuum from 10.0 to 13.0psi @13:42:29.56 SS.reconfigure :inhaling 1.13ml/s @13:42:29.61 SS.seek 25ml @13:42:54.24 Stroke completed -- waiting for pressure to equalize ... @13:42:54.25 SS.reconfigure :exhale @13:42:54.30 SS.hold @13:43:12.67 Intake.select :close @13:43:12.82 SSV.dial :exhaust @13:43:13.59 Exhaust.select :open @13:43:13.70 SS.seek 0ml @13:43:22.43 Exhaust.select :close @13:43:22.54 SSV.dial CBV @13:43:22.56
CBV.dial SSV @13:43:23.34 Allowing 130s for 25.0ml of remaining 250.0ml @13:43:23.35 Intake.select :open @13:43:25.93 Intake @16.2psia, vacuum from 10.0 to 13.0psi @13:43:25.94 SS.reconfigure :inhaling 1.13ml/s @13:43:25.99 SS.seek 25ml @13:43:50.68 Stroke completed -- waiting for pressure to equalize ... @13:43:50.69 SS.reconfigure :exhale @13:43:50.74 SS.hold @13:44:09.07 Intake.select :close @13:44:09.22 SSV.dial :exhaust @13:44:09.98 Exhaust.select :open @13:44:10.09 SS.seek 0ml @13:44:19.07 Exhaust.select :close @13:44:19.18 SSV.dial CBV @13:44:19.20
CBV.dial SSV @13:44:19.98 Allowing 130s for 25.0ml of remaining 225.0ml @13:44:19.99 Intake.select :open @13:44:22.15 Intake @16.2psia, vacuum from 10.0 to 13.0psi @13:44:22.16 SS.reconfigure :inhaling 1.13ml/s @13:44:22.21 SS.seek 25ml @13:44:46.24 Stroke completed -- waiting for pressure to equalize ... @13:44:46.25 SS.reconfigure :exhale @13:44:46.30 SS.hold @13:45:07.00 Intake.select :close @13:45:07.15 SSV.dial :exhaust @13:45:07.91 Exhaust.select :open @13:45:08.03 SS.seek 0ml @13:45:16.80 Exhaust.select :close @13:45:16.91 SSV.dial CBV @13:45:16.95
CBV.dial SSV @13:45:17.69 Allowing 130s for 25.0ml of remaining 200.0ml @13:45:17.70 Intake.select :open @13:45:19.87 Intake @16.3psia, vacuum from 10.0 to 13.0psi @13:45:19.88 SS.reconfigure :inhaling 1.13ml/s @13:45:19.93 SS.seek 25ml @13:45:43.20 Vacuum too high -- waiting up to 40 seconds @13:45:43.21 SS.reconfigure :exhale @13:45:43.27 SS.hold @13:45:51.07 Intake @16.5psia, vacuum from 10.0 to 13.0psi @13:45:51.09 SS.reconfigure :inhaling 0.91ml/s @13:45:51.14 SS.seek 25ml @13:45:53.22 Stroke completed -- waiting for pressure to equalize ... @13:45:53.23 SS.reconfigure :exhale @13:45:53.28 SS.hold @13:46:05.94 Intake.select :close @13:46:06.46 SSV.dial :exhaust @13:46:07.29 Exhaust.select :open @13:46:07.40 SS.seek 0ml @13:46:16.16 Exhaust.select :close @13:46:16.27 SSV.dial CBV @13:46:16.29
CBV.dial SSV @13:46:17.08 Allowing 130s for 25.0ml of remaining 175.0ml @13:46:17.09 Intake.select :open @13:46:19.26 Intake @16.4psia, vacuum from 10.0 to 13.0psi @13:46:19.28 SS.reconfigure :inhaling 0.91ml/s @13:46:19.33 SS.seek 25ml @13:46:49.16 Stroke completed -- waiting for pressure to equalize ... @13:46:49.17 SS.reconfigure :exhale @13:46:49.22 SS.hold @13:47:09.60 Intake.select :close @13:47:09.75 SSV.dial :exhaust @13:47:10.51 Exhaust.select :open @13:47:10.63 SS.seek 0ml @13:47:19.57 Exhaust.select :close @13:47:20.10 SSV.dial CBV @13:47:20.13
CBV.dial SSV @13:47:20.89 Allowing 130s for 25.0ml of remaining 150.0ml @13:47:20.91 Intake.select :open @13:47:23.07 Intake @16.2psia, vacuum from 10.0 to 13.0psi @13:47:23.08 SS.reconfigure :inhaling 0.91ml/s @13:47:23.13 SS.seek 25ml @13:47:52.42 Stroke completed -- waiting for pressure to equalize ... @13:47:52.43 SS.reconfigure :exhale @13:47:52.48 SS.hold @13:48:12.90 Intake.select :close @13:48:13.06 SSV.dial :exhaust @13:48:13.82 Exhaust.select :open @13:48:13.94 SS.seek 0ml @13:48:22.83 Exhaust.select :close @13:48:22.94 SSV.dial CBV @13:48:22.96
CBV.dial SSV @13:48:23.75 Allowing 130s for 25.0ml of remaining 125.0ml @13:48:23.76 Intake.select :open @13:48:26.35 Intake @16.3psia, vacuum from 10.0 to 13.0psi @13:48:26.37 SS.reconfigure :inhaling 0.91ml/s @13:48:26.41 SS.seek 25ml @13:48:55.91 Stroke completed -- waiting for pressure to equalize ... @13:48:55.92 SS.reconfigure :exhale @13:48:55.97 SS.hold @13:49:16.27 Intake.select :close @13:49:16.42 SSV.dial :exhaust @13:49:17.18 Exhaust.select :open @13:49:17.30 SS.seek 0ml @13:49:25.95 Exhaust.select :close @13:49:26.06 SSV.dial CBV @13:49:26.09
CBV.dial SSV @13:49:26.86 Allowing 130s for 25.0ml of remaining 99.9ml @13:49:26.87 Intake.select :open @13:49:29.04 Intake @16.4psia, vacuum from 10.0 to 13.0psi @13:49:29.05 SS.reconfigure :inhaling 0.91ml/s @13:49:29.10 SS.seek 25ml @13:49:53.07 Vacuum too high -- waiting up to 40 seconds @13:49:53.47 SS.reconfigure :exhale @13:49:53.57 SS.hold @13:49:59.29 Intake @16.6psia, vacuum from 10.0 to 13.0psi @13:49:59.30 SS.reconfigure :inhaling 0.73ml/s @13:49:59.35 SS.seek 25ml @13:50:07.09 Stroke completed -- waiting for pressure to equalize ... @13:50:07.10 SS.reconfigure :exhale @13:50:07.15 SS.hold @13:50:21.52 Intake.select :close @13:50:21.67 SSV.dial :exhaust @13:50:22.44 Exhaust.select :open @13:50:22.55 SS.seek 0ml @13:50:31.22 Exhaust.select :close @13:50:31.33 SSV.dial CBV @13:50:31.35
CBV.dial SSV @13:50:32.14 Allowing 130s for 25.0ml of remaining 74.9ml @13:50:32.15 Intake.select :open @13:50:34.32 Intake @16.4psia, vacuum from 10.0 to 13.0psi @13:50:34.34 SS.reconfigure :inhaling 0.73ml/s @13:50:34.38 SS.seek 25ml @13:51:10.78 Stroke completed -- waiting for pressure to equalize ... @13:51:10.79 SS.reconfigure :exhale @13:51:10.84 SS.hold @13:51:31.25 Intake.select :close @13:51:31.82 SSV.dial :exhaust @13:51:32.60 Exhaust.select :open @13:51:32.72 SS.seek 0ml @13:51:41.48 Exhaust.select :close @13:51:41.59 SSV.dial CBV @13:51:41.61
CBV.dial SSV @13:51:42.41 Allowing 130s for 25.0ml of remaining 49.9ml @13:51:42.42 Intake.select :open @13:51:44.58 Intake @16.2psia, vacuum from 10.0 to 13.0psi @13:51:44.60 SS.reconfigure :inhaling 0.73ml/s @13:51:44.65 SS.seek 25ml @13:52:21.11 Stroke completed -- waiting for pressure to equalize ... @13:52:21.12 SS.reconfigure :exhale @13:52:21.17 SS.hold @13:52:41.45 Intake.select :close @13:52:41.60 SSV.dial :exhaust @13:52:42.37 Exhaust.select :open @13:52:42.48 SS.seek 0ml @13:52:51.76 Exhaust.select :close @13:52:51.87 SSV.dial CBV @13:52:51.91
CBV.dial SSV @13:52:52.65 Allowing 130s for 24.9ml of remaining 24.9ml @13:52:52.67 Intake.select :open @13:52:54.83 Intake @16.4psia, vacuum from 10.0 to 13.0psi @13:52:54.85 SS.reconfigure :inhaling 0.73ml/s @13:52:54.89 SS.seek 24.95ml @13:53:30.89 Stroke completed -- waiting for pressure to equalize ... @13:53:30.90 SS.reconfigure :exhale @13:53:30.95 SS.hold @13:53:53.28 Intake.select :close @13:53:53.44 SSV.dial :exhaust @13:53:54.20 Exhaust.select :open @13:53:54.32 SS.seek 0ml @13:54:03.31 Exhaust.select :close @13:54:03.42 Sampled 1000.0ml @13:54:03.43 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @13:54:03.80 Intake.select :close @13:54:03.93 CSV.dial :waste @13:54:04.09 SSV.dial CBV,avoiding: :exhaust @13:54:04.11 CS.seek 0ml @13:54:04.14
CBV.dial SSV @13:54:04.27 CSV.dial :air @13:54:05.02 CS.seek 10ml @13:54:06.03
CTV.dial :air @13:54:06.82 delay 15 seconds @13:54:21.85 SSV.dial :spit,avoiding: :exhaust @13:54:23.16 CTV.dial CSV,avoiding: :intake @13:54:23.19
SS.seek 0ml @13:54:23.32 SSV.dial CBV,avoiding: :exhaust @13:54:29.54 SSV.dial between CBV and plug @13:54:30.03 CBV.dial between SSV and waste2 @13:54:30.41 CSV.dial between air and CTV @13:54:30.72 CS.seek 8.413ml @13:54:35.23 CTV.dial CSV,avoiding: :air @13:54:35.26
CSV.dial CTV @13:54:35.59 CS.reconfigure :pressurize @13:54:35.65 CS.seek 0ml @13:54:49.83 BubblePoint: Intake @24.1psia, Outlet @1.0psia @13:54:49.84 CBV.dial SSV @13:54:50.17 SS.reconfigure :default @13:54:50.22 SS.seek 10ml @13:54:55.76 delay 15 seconds @13:55:10.89 Evacuated: Intake @23.6psia, Outlet @23.5psia @13:55:10.90 SSV.dial :spit,avoiding: :exhaust @13:55:11.94 SS.reconfigure :exhale @13:55:11.99 SS.seek 0ml @13:55:16.98 SS.reconfigure :fast @13:55:17.02 SS.seek 5ml @13:55:19.80 SSV.dial CBV,avoiding: :exhaust @13:55:19.82
CBV.dial SSV @13:55:21.16 CTV.dial :air,avoiding: :intake @13:55:22.51 CSV.dial CTV @13:55:22.54
SS.seek 10ml @13:55:23.68 CSV.dial :air @13:55:24.12 CS.reconfigure :fast @13:55:24.17 CS.seek 0ml @13:55:25.08
SSV.dial :spit,avoiding: :exhaust @13:55:26.41 SS.reconfigure :exhale @13:55:26.46 SS.seek 0ml @13:55:31.72 SS.reconfigure :fast @13:55:31.83 SS.seek 5ml @13:55:32.07 CS.stop @13:55:34.59 SSV.dial CBV,avoiding: :exhaust @13:55:34.62
CBV.dial SSV @13:55:36.95 SSV.dial :spit,avoiding: :exhaust @13:55:38.27 SS.reconfigure :default @13:55:38.32 SS.seek 0ml @13:55:41.52 SS.stop @13:55:41.56 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @13:55:41.57 Intake.select :close @13:55:41.64 CSV.dial :waste @13:55:41.76 SSV.dial CBV,avoiding: :exhaust @13:55:41.78
CBV.dial SSV @13:55:42.42 CS.seek 0ml @13:55:42.49 CSV.dial :air @13:55:43.12
CTV.dial :air @13:55:43.20 delay 15 seconds @13:55:43.24 CS.seek 10ml @13:55:58.22
SSV.dial :spit,avoiding: :exhaust @13:55:59.54 CTV.dial CSV,avoiding: :intake @13:55:59.56
SS.reconfigure :exhale @13:55:59.67 SS.seek 0ml @13:56:00.06 SSV.dial CBV,avoiding: :exhaust @13:56:07.73 SSV.dial between CBV and plug @13:56:08.22 CBV.dial between SSV and waste2 @13:56:08.60 CSV.dial between air and CTV @13:56:08.92 CS.seek 7.888ml @13:56:14.58 CTV.dial CSV,avoiding: :air @13:56:14.62
CSV.dial CTV @13:56:14.97 CS.reconfigure :pressurize @13:56:15.02 CS.seek 0ml @13:56:30.14 BubblePoint: Intake @24.1psia, Outlet @2.8psia @13:56:30.15 CBV.dial SSV @13:56:30.48 SS.reconfigure :default @13:56:30.53 SS.seek 10ml @13:56:36.07 delay 15 seconds @13:56:51.20 Evacuated: Intake @23.9psia, Outlet @22.7psia @13:56:51.21 SSV.dial :spit,avoiding: :exhaust @13:56:52.27 SS.reconfigure :exhale @13:56:52.31 SS.seek 0ml @13:56:57.32 SS.reconfigure :fast @13:56:57.37 SS.seek 5ml @13:57:00.28 SSV.dial CBV,avoiding: :exhaust @13:57:00.31
CBV.dial SSV @13:57:01.64 CTV.dial :air,avoiding: :intake @13:57:03.40 CSV.dial CTV @13:57:03.43
SS.seek 10ml @13:57:04.64 CSV.dial :air @13:57:05.09 CS.reconfigure :fast @13:57:05.14 CS.seek 0ml @13:57:06.07
SSV.dial :spit,avoiding: :exhaust @13:57:07.40 SS.reconfigure :exhale @13:57:07.45 SS.seek 0ml @13:57:10.94 CS.stop @13:57:12.65
SS.reconfigure :fast @13:57:12.70 SS.seek 5ml @13:57:15.56 SSV.dial CBV,avoiding: :exhaust @13:57:15.58
CBV.dial SSV @13:57:17.94 SSV.dial :spit,avoiding: :exhaust @13:57:19.26 SS.reconfigure :default @13:57:19.31 SS.seek 0ml @13:57:22.43 SS.stop @13:57:22.47 Lysing: Adding 2.0ml AqMeOH @13:57:22.48 CBV.dial between CSV and waste1,avoiding: :DBV @13:57:23.99 CTV.dial CSV @13:57:25.32 CSV.dial CSR @13:57:26.23 Pulling 1ml of CSR.AqMeOH @13:57:26.24 CSR.select :AqMeOH @13:57:26.35 CS.reconfigure :default @13:57:26.40 CS.seek 1ml @13:57:30.07 delay 5 seconds @13:57:35.09 CSR.select :air @13:57:35.26 CS.seek 6ml @13:57:48.85 delay 2 seconds @13:57:50.87 CSV.dial :waste2 @13:57:51.34 CS.reconfigure :fast @13:57:51.39 CS.seek 0ml @13:58:05.69 CSV.dial CSR @13:58:06.15 Pulling 2ml of CSR.AqMeOH @13:58:06.16 CSR.select :AqMeOH @13:58:06.27 CS.reconfigure :default @13:58:06.32 CS.seek 2ml @13:58:12.35 delay 5 seconds @13:58:17.37 CSR.select :air @13:58:17.53 CS.seek 5ml @13:58:26.04 delay 2 seconds @13:58:28.05 CSV.dial CTV @13:58:28.51 CS.seek 1ml @13:58:39.83 Before lysing Collection Puck Heater at 11.6C/26.2psia @13:58:39.85 Heating Puck.from 5, :sh1 in Collection Clamp to 70C for 10:00 @13:58:39.87 CH.seek 70C,8:00 @14:00:53.99 CH.hold 10:00,70C @14:00:54.19 Begun lysing Collection Puck Heater at 68.1C/33.2psia @14:10:54.20 Ended lysing Collection Puck Heater at 70C/32.3psia @14:10:54.22 CS.seek 0ml @14:10:57.85 CTV.dial between spare and air,avoiding: :intake @14:10:58.89 CSV.dial :air @14:10:59.33 CS.seek 5ml @14:11:13.12 CSV.dial CTV @14:11:13.58 delay 2 seconds @14:11:15.59 CSV.dial :air @14:11:16.04 delay 2 seconds @14:11:18.06 CSV.dial CTV @14:11:18.52 delay 2 seconds @14:11:20.54 CSV.dial :air @14:11:20.98 delay 2 seconds @14:11:23.00 CS.seek 0ml @14:11:36.73 Puck @62.7psia after final lysis pressurization @14:11:36.75 Recovering Lysate into Collection syringe @14:11:36.76 CSV.dial CBV @14:11:37.78 CBV.dial CSV @14:11:38.15 CS.seek 5ml @14:11:51.75 delay 5 seconds @14:11:56.76 CTV.dial :air @14:11:57.28 CS.seek 7ml @14:12:03.15 delay 5 seconds @14:12:08.17 CSV.dial :air2 @14:12:08.61 CS.seek 10ml @14:12:17.09 CSV.dial between air and CTV,avoiding: CBV @14:12:18.37 Lysing Complete @14:12:18.44 Storing sh1 puck in Collection Clamp to tube 4 @14:12:18.61 SC.reconfigure :tight @14:12:18.66 SC.seek 4 @14:12:22.24 SC.configure :limp @14:12:22.45 Elbow.reconfigure :tight @14:12:22.50 Elbow.seek 4 @14:12:24.25 Forearm.reconfigure :tight @14:12:24.30 Forearm.seek 4 + 450 counts @14:12:25.76 SE.reconfigure :pick @14:12:25.82 SE.seek :beamBreak @14:12:27.05 SE.configure :default @14:12:27.21 Elbow.reconfigure :limp @14:12:27.27 Forearm.reconfigure :loose @14:12:27.32 Forearm.seek 4 @14:12:27.58 Elbow.hold @14:12:27.65 Elbow.reconfigure :tight @14:12:27.75 SE.seek 28.11 Pucks below Top Plate @14:12:28.29 Forearm.force -20 @14:12:28.33 Forearm.seek 4 + 285 counts @14:12:28.61 Forearm.reconfigure :tight @14:12:28.66 Forearm.seek 4 + 450 counts @14:12:28.86 SE.seek 29.11 Pucks below Top Plate @14:12:29.45 Forearm.reconfigure :loose @14:12:29.50 Forearm.seek :retract @14:12:30.42 Elbow.seek CC @14:12:32.13 Elbow.reconfigure :loose @14:12:32.23 Forearm.seek CC @14:12:33.19 Hand.close @14:12:33.84 CC.seek :open @14:12:38.57 Forearm.seek :retract @14:12:39.56 Elbow.reconfigure :tight @14:12:39.60 Elbow.seek 4 @14:12:41.37 Elbow.reconfigure :limp @14:12:41.42 Forearm.seek 4 @14:12:42.44 Elbow.hold @14:12:42.51 Elbow.reconfigure :tight @14:12:42.56 Hand.open @14:12:43.33 Forearm.force -20 @14:12:43.75 Forearm.seek 4 + 318 counts @14:12:44.09 Forearm.reconfigure :tight @14:12:44.14 Forearm.seek 4 + 450 counts @14:12:44.42 SE.seek :down @14:12:45.19 SE.stop @14:12:45.55
Forearm.reconfigure :loose @14:12:45.59 Forearm.seek :retract @14:12:46.51 Tranferring DA @14:12:46.52 Beginning samplePassC @14:12:46.53 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:12:46.72 Elbow.seek FlushPuck::Garage @14:12:48.56 Elbow.reconfigure :loose @14:12:48.66 Forearm.seek :garage @14:12:49.66 Hand.close @14:12:50.45 Forearm.seek :retract @14:12:51.43 Elbow.reconfigure :tight @14:12:51.48 Elbow.seek CC @14:12:54.42 Elbow.reconfigure :loose @14:12:54.52 Forearm.reconfigure :tight @14:12:54.57 Forearm.seek CC @14:12:55.94 CC.seek :closed @14:13:00.46 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.38 @14:13:00.52 Hand.open @14:13:01.16 Forearm.reconfigure :loose @14:13:01.21 Forearm.seek :retract @14:13:02.24 PSV.dial PSR @14:13:03.62 Pulling 1.5ml of PSR.flush @14:13:03.63 PSR.select :flush @14:13:03.75 PS.seek 1.5ml @14:13:19.87 delay 5 seconds @14:13:24.89 PSV.dial :waste1 @14:13:25.36 PS.seek 0ml @14:13:41.43 PSV.dial PSR @14:13:41.90 PSR.select :air @14:13:42.02 PS.seek 2.5ml @14:14:08.32 PRV.dial CBV,avoiding: :ab2 @14:14:08.35
CBV.dial PRV @14:14:10.09 PSV.dial :mixloop @14:14:11.14 CTV.dial :air,avoiding: :intake @14:14:11.21 PTV.dial :PRVmixing @14:14:12.54 PS.seek 0ml @14:14:38.87 PSV.dial :air @14:14:39.35 PS.seek 2.5ml @14:15:05.68 PSV.dial :mixloop @14:15:06.16 PS.seek 0ml @14:15:32.65 PSV.dial :air @14:15:33.13 PS.seek 2.5ml @14:15:59.43 PSV.dial :mixloop @14:15:59.91 PS.seek 0ml @14:16:26.43 Garaging FlushPuck in Collection Clamp @14:16:26.50 Elbow.reconfigure :tight @14:16:26.55 Elbow.seek CC @14:16:26.84 Elbow.reconfigure :loose @14:16:26.94 Forearm.seek CC @14:16:27.94 Hand.close @14:16:28.59 CC.seek :open @14:16:33.20 Forearm.seek :retract @14:16:34.27 Elbow.reconfigure :tight @14:16:34.32 Elbow.seek :garage @14:16:37.62 Elbow.reconfigure :loose @14:16:37.78 Forearm.reconfigure :tight @14:16:37.83 Forearm.seek :garage @14:16:38.88 Hand.open @14:16:39.52 Forearm.reconfigure :loose @14:16:39.57 Forearm.seek :retract @14:16:40.57 PSV.dial :mixloop @14:16:40.64 PTV.dial :PRVmixing @14:16:40.72 PRV.dial CBV @14:16:40.74
CBV.dial PRV @14:16:40.88 CTV.dial :air,avoiding: :intake @14:16:40.96 Loading lyfil puck from tube 5 into Collection Clamp @14:16:41.20 SC.reconfigure :tight @14:16:41.25 SC.seek 5 @14:16:44.82 SC.configure :limp @14:16:45.03 Elbow.reconfigure :tight @14:16:45.08 Elbow.seek 5 @14:16:47.04 Forearm.reconfigure :tight @14:16:47.09 Forearm.seek 5 + 450 counts @14:16:48.09 SE.reconfigure :pick @14:16:48.15 SE.seek :beamBreak @14:16:51.08 SE.configure :default @14:16:51.24 Elbow.seek 5 @14:16:51.42 Forearm.seek 5 + 300 counts @14:16:51.71 Forearm.reconfigure :loose @14:16:51.75 Hand.close @14:16:52.35 SE.seek :down @14:16:52.55
Forearm.seek :retract @14:16:53.88 Elbow.seek CC @14:16:54.98 SE.stop @14:16:55.51
Elbow.reconfigure :loose @14:16:55.62 Forearm.reconfigure :tight @14:16:55.67 Forearm.seek CC @14:16:56.88 CC.seek :closed @14:17:01.41 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.18 @14:17:01.42 Hand.open @14:17:02.14 Forearm.reconfigure :loose @14:17:02.19 Forearm.seek :retract @14:17:03.15 CTV.dial CSV,avoiding: :intake @14:17:04.51 CSV.dial CTV @14:17:04.81 PS.seek 2.5ml @14:17:31.24 CS.seek 4.5ml @14:17:46.15 delay 8 seconds @14:17:54.17 PSV.dial :air @14:17:54.64 delay 2 seconds @14:17:56.65 PSV.dial :waste2 @14:17:57.52 PS.seek 0ml @14:18:23.90 CBV.dial between PRV and waste1 @14:18:24.22 CSV.dial :air @14:18:24.68 CS.seek 10ml @14:18:39.52 CSV.dial CTV @14:18:39.98 CS.seek 0ml @14:19:06.39 CBV.dial :waste1 @14:19:06.72 delay 5 seconds @14:19:11.75 CTV.dial :air,avoiding: :intake @14:19:13.17 Storing lyfil puck in Collection Clamp to tube 4 @14:19:13.34 SC.reconfigure :tight @14:19:13.82 SC.seek 4 @14:19:17.43 SC.configure :limp @14:19:17.65 Elbow.reconfigure :tight @14:19:17.70 Elbow.seek 4 @14:19:19.59 Forearm.reconfigure :tight @14:19:19.64 Forearm.seek 4 + 450 counts @14:19:20.67 SE.reconfigure :pick @14:19:20.73 SE.seek :beamBreak @14:19:22.00 SE.configure :default @14:19:22.16 Elbow.reconfigure :limp @14:19:22.21 Forearm.reconfigure :loose @14:19:22.25 Forearm.seek 4 @14:19:22.48 Elbow.hold @14:19:22.54 Elbow.reconfigure :tight @14:19:22.64 SE.seek 29.12 Pucks below Top Plate @14:19:23.09 Forearm.force -20 @14:19:23.13 Forearm.seek 4 + 319 counts @14:19:23.41 Forearm.reconfigure :tight @14:19:23.46 Forearm.seek 4 + 450 counts @14:19:23.66 SE.seek 30.12 Pucks below Top Plate @14:19:24.24 Forearm.reconfigure :loose @14:19:24.29 Forearm.seek :retract @14:19:25.21 Elbow.seek CC @14:19:26.85 Elbow.reconfigure :loose @14:19:26.95 Forearm.seek CC @14:19:27.91 Hand.close @14:19:28.46 CC.seek :open @14:19:33.62 Forearm.seek :retract @14:19:34.65 Elbow.reconfigure :tight @14:19:34.69 Elbow.seek 4 @14:19:36.46 Elbow.reconfigure :limp @14:19:36.51 Forearm.seek 4 @14:19:37.47 Elbow.hold @14:19:37.54 Elbow.reconfigure :tight @14:19:37.58 Hand.open @14:19:38.33 Forearm.force -20 @14:19:38.37 Forearm.seek 4 + 314 counts @14:19:38.78 Forearm.reconfigure :tight @14:19:38.83 Forearm.seek 4 + 450 counts @14:19:39.08 SE.seek :down @14:19:39.74 SE.stop @14:19:40.23
Forearm.reconfigure :loose @14:19:40.28 Forearm.seek :retract @14:19:41.24 PSV.dial :air @14:19:41.98 PS.seek 2.5ml @14:20:08.37 delay 1 second @14:20:09.38 PSV.dial :mixloop @14:20:09.84 delay 1 second @14:20:10.85 PSV.dial :air @14:20:11.32 delay 1 second @14:20:12.34 PSV.dial :mixloop @14:20:12.81 delay 1 second @14:20:13.82 PSV.dial :air @14:20:14.29 delay 1 second @14:20:15.31 PS.seek 0ml @14:20:41.59 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @14:20:41.77 Elbow.seek FlushPuck::Garage @14:20:43.63 Elbow.reconfigure :loose @14:20:43.73 Forearm.seek :garage @14:20:45.17 Hand.close @14:20:45.95 Forearm.seek :retract @14:20:46.93 Elbow.reconfigure :tight @14:20:46.97 Elbow.seek PC @14:20:48.25 Elbow.reconfigure :loose @14:20:48.35 Forearm.reconfigure :tight @14:20:48.40 Forearm.seek PC @14:20:49.67 PC.seek :closed @14:20:54.28 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.65 @14:20:54.35 Hand.open @14:20:55.02 Forearm.reconfigure :loose @14:20:55.06 Forearm.seek :retract @14:20:56.00 PRV.dial :air @14:20:56.44 PSV.dial :mixloop @14:20:56.98 PS.seek 0.3ml @14:21:00.86 PSV.dial PSR @14:21:01.93 Pulling 0.4ml of PSR.air @14:21:01.94 PSR.select :air @14:21:01.96 PS.seek 0.7ml @14:21:06.90 delay 3 seconds @14:21:09.91 PSR.select :air @14:21:09.94 PS.seek 2.5ml @14:21:29.12 delay 3 seconds @14:21:32.13 PSV.dial :mixloop @14:21:33.19 PTV.dial :mixing @14:21:33.96 PBV.dial :waste2 @14:21:34.93 PS.seek 0ml @14:22:01.24 Garaging FlushPuck in Processing Clamp @14:22:01.31 Elbow.reconfigure :tight @14:22:01.36 Elbow.seek PC @14:22:01.90 Elbow.reconfigure :loose @14:22:02.02 Forearm.seek PC @14:22:02.97 Hand.close @14:22:03.58 PC.seek :open @14:22:08.30 Forearm.seek :retract @14:22:09.30 Elbow.reconfigure :tight @14:22:09.35 Elbow.seek :garage @14:22:10.70 Elbow.reconfigure :loose @14:22:10.80 Forearm.reconfigure :tight @14:22:10.85 Forearm.seek :garage @14:22:11.92 Hand.open @14:22:12.64 Forearm.reconfigure :loose @14:22:12.69 Forearm.seek :retract @14:22:13.66 Loading da puck from tube 5 into Processing Clamp @14:22:13.88 SC.reconfigure :tight @14:22:13.93 SC.seek 5 @14:22:17.48 SC.configure :limp @14:22:17.69 Elbow.reconfigure :tight @14:22:17.74 Elbow.seek 5 @14:22:19.60 Forearm.reconfigure :tight @14:22:19.65 Forearm.seek 5 + 450 counts @14:22:20.67 SE.reconfigure :pick @14:22:20.73 SE.seek :beamBreak @14:22:23.74 SE.configure :default @14:22:23.90 Elbow.seek 5 @14:22:24.08 Forearm.seek 5 + 300 counts @14:22:24.33 Forearm.reconfigure :loose @14:22:24.37 Hand.close @14:22:24.97 SE.seek :down @14:22:25.60
Forearm.seek :retract @14:22:26.56 Elbow.seek PC @14:22:27.67 SE.stop @14:22:29.13
Elbow.reconfigure :loose @14:22:29.23 Forearm.reconfigure :tight @14:22:29.28 Forearm.seek PC @14:22:30.59 PC.seek :closed @14:22:35.22 Processing Clamp onSpring/offSpring for 15/25 samples, on/off torque 1.66 @14:22:35.23 Hand.open @14:22:35.90 Forearm.reconfigure :loose @14:22:35.94 Forearm.seek :retract @14:22:36.94 Heating Puck.from 5, :da in Processing Clamp to 28C for 2:00:00 @14:22:36.96 PH.seek 28C,5:00 @14:23:22.79 PH.hold 2:00:00,28C @14:23:22.83 delay 1:30 @14:24:52.87 PSR.select :air @14:24:52.89 PSV.dial PSR @14:24:53.94 Pulling 0.5ml of PSR.wash @14:24:53.95 PSR.select :wash @14:24:54.06 PS.seek 0.5ml @14:25:00.05 delay 5 seconds @14:25:05.07 PSR.select :air @14:25:05.19 PS.seek 2ml @14:25:21.39 PTV.dial :puck @14:25:22.14 PSV.dial PTV @14:25:22.91 PBV.dial :waste1 @14:25:24.31 PS.seek 1.5ml @14:25:30.24 delay 3 seconds @14:25:33.25 PBV.dial between PSV and waste1 @14:25:33.62 PS.seek 0ml @14:25:49.80 delay 5:00 @14:30:49.82 recoverPSRreagent08 @14:30:49.83 PTV.dial :puck @14:30:49.84 PBV.dial :waste1 @14:30:50.21 delay 30 seconds @14:31:20.24 PBV.dial between PSV and waste1 @14:31:20.60 PSR.select :air @14:31:20.62 PSV.dial PSR @14:31:21.39 PS.reconfigure :fast @14:31:21.44 PS.seek 2.5ml @14:31:45.32 PSV.dial PTV @14:31:46.09 delay 5 seconds @14:31:51.11 PSV.dial PSR @14:31:51.85 delay 5 seconds @14:31:56.87 PSV.dial PTV @14:31:57.64 delay 5 seconds @14:32:02.65 PSV.dial :air @14:32:03.42 PS.seek 0ml @14:32:26.51 PTV.dial :PRVmixing,via: PRV @14:32:27.84 PSV.dial :mixloop @14:32:28.39 PRV.dial :ab1 @14:32:29.14 PS.reconfigure :slow @14:32:29.19 PS.seek 0.15ml @14:32:32.79 delay 2 seconds @14:32:34.81 CBV.dial PRV @14:32:34.83
PRV.dial CBV @14:32:35.42 PS.seek 0.35ml @14:32:40.04 delay 2 seconds @14:32:42.06 CBV.dial between PRV and PSV @14:32:42.37 PRV.dial :air @14:32:42.83 PS.seek 0.9ml @14:32:54.51 PRV.dial :waste2 @14:32:55.27 PS.seek 0.5ml @14:33:03.98 addPMLReagent08 @14:33:03.99 PSV.dial :air @14:33:04.45 PS.reconfigure :default @14:33:04.50 PS.seek 2ml @14:33:20.69 PSV.dial :PML @14:33:21.16 PTV.dial :mixing @14:33:21.91 PBV.dial :waste1 @14:33:22.35 PS.seek 1.85ml @14:33:24.79 PBV.dial between PSV and waste1 @14:33:25.17 PS.seek 0ml @14:33:45.07 delay 2 seconds @14:33:47.09 Beginning samplePassC @14:33:47.10 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:33:47.28 Elbow.reconfigure :tight @14:33:47.33 Elbow.seek FlushPuck::Garage @14:33:48.64 Elbow.reconfigure :loose @14:33:48.74 Forearm.seek :garage @14:33:49.74 Hand.close @14:33:50.45 Forearm.seek :retract @14:33:51.43 Elbow.reconfigure :tight @14:33:51.48 Elbow.seek CC @14:33:54.41 Elbow.reconfigure :loose @14:33:54.51 Forearm.reconfigure :tight @14:33:54.56 Forearm.seek CC @14:33:56.22 CC.seek :closed @14:34:00.73 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.44 @14:34:00.80 Hand.open @14:34:01.50 Forearm.reconfigure :loose @14:34:01.55 Forearm.seek :retract @14:34:02.55 PSV.dial PSR @14:34:03.74 Pulling 1.5ml of PSR.flush @14:34:03.75 PSR.select :flush @14:34:03.87 PS.seek 1.5ml @14:34:20.01 delay 5 seconds @14:34:25.03 PSV.dial :waste1 @14:34:25.50 PS.seek 0ml @14:34:41.57 PSV.dial PSR @14:34:42.04 PSR.select :air @14:34:42.16 PS.seek 2.5ml @14:35:08.57 PRV.dial CBV,avoiding: :ab2 @14:35:08.59
CBV.dial PRV @14:35:10.26 PSV.dial :mixloop @14:35:11.30 CTV.dial :air,avoiding: :intake @14:35:11.38 PTV.dial :PRVmixing @14:35:12.17 PS.seek 0ml @14:35:38.53 PSV.dial :air @14:35:39.01 PS.seek 2.5ml @14:36:05.35 PSV.dial :mixloop @14:36:05.82 PS.seek 0ml @14:36:32.19 PSV.dial :air @14:36:32.67 PS.seek 2.5ml @14:36:59.00 PSV.dial :mixloop @14:36:59.48 PS.seek 0ml @14:37:25.79 Garaging FlushPuck in Collection Clamp @14:37:25.85 Elbow.reconfigure :tight @14:37:25.90 Elbow.seek CC @14:37:26.66 Elbow.reconfigure :loose @14:37:26.76 Forearm.seek CC @14:37:27.83 Hand.close @14:37:28.45 CC.seek :open @14:37:33.06 Forearm.seek :retract @14:37:34.08 Elbow.reconfigure :tight @14:37:34.13 Elbow.seek :garage @14:37:36.97 Elbow.reconfigure :loose @14:37:37.07 Forearm.reconfigure :tight @14:37:37.11 Forearm.seek :garage @14:37:38.13 Hand.open @14:37:38.75 Forearm.reconfigure :loose @14:37:38.80 Forearm.seek :retract @14:37:39.82 delayUntil "14:41:47.08" @14:37:39.85 Whole Cell Archive sampling 1000ml @14:37:39.86 Loading wcr puck from tube 5 into Collection Clamp @14:37:40.33 Elbow.reconfigure :tight @14:37:40.38 Elbow.seek 5 @14:37:42.39 Forearm.reconfigure :tight @14:37:42.44 Forearm.seek 5 + 450 counts @14:37:43.56 SE.reconfigure :pick @14:37:43.62 SE.seek :beamBreak @14:37:46.66 SE.configure :default @14:37:46.82 Elbow.seek 5 @14:37:47.14 Forearm.seek 5 + 300 counts @14:37:47.30 Forearm.reconfigure :loose @14:37:47.79 Hand.close @14:37:48.46 SE.seek :down @14:37:48.68 Forearm.seek :retract @14:37:49.63 Elbow.seek CC @14:37:51.22 Elbow.reconfigure :loose @14:37:51.29 SE.stop @14:37:51.40 Forearm.reconfigure :tight @14:37:51.45 Forearm.seek CC @14:37:52.78 CC.seek :closed @14:37:57.31 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.46 @14:37:57.33 Hand.open @14:37:58.00 Forearm.reconfigure :loose @14:37:58.05 Forearm.seek :retract @14:37:59.04 collectSample 1000ml w/bubble point of 35psi @14:37:59.05 Priming 7ml @14:37:59.06 Intake.select :close @14:37:59.07 Exhaust.select :close @14:37:59.08 SS.hold @14:37:59.29 Calibrating pressure sensors @14:37:59.30 CTV.dial :air @14:37:59.38 SSV.dial :spit,avoiding: :exhaust @14:37:59.47 SS.reconfigure :fast @14:37:59.52 SS.seek 5ml @14:38:02.48 SSV.dial CBV @14:38:02.50 CBV.dial SSV @14:38:03.89 delay 3.8 seconds @14:38:07.82 Sensor limits: Intake max 102.4psia; Outlet max 93.6psia @14:38:07.83 SSV.dial :spit @14:38:09.61 SS.seek 0ml @14:38:12.29 SSV.dial CBV @14:38:13.62 CTV.dial :intake @14:38:14.40 SSV.dial CBV @14:38:14.42 CBV.dial SSV @14:38:14.57 Allowing 40s for 7.0ml of remaining 7.0ml @14:38:14.59 Intake.select :open @14:38:16.75 Intake @14.5psia, vacuum from 9.0 to 12.0psi @14:38:16.77 SS.reconfigure :inhaling 2.20ml/s @14:38:16.82 SS.seek 7ml @14:38:23.55 Stroke completed -- waiting for pressure to equalize ... @14:38:23.57 SS.reconfigure :exhale @14:38:23.61 SS.hold @14:38:46.58 Intake.select :close @14:38:46.74 SSV.dial :exhaust @14:38:47.52 Exhaust.select :open @14:38:47.64 SS.seek 0ml @14:38:51.72 Exhaust.select :close @14:38:51.83 Sampling 1000.0ml @14:38:51.85 Intake.select :close @14:38:51.86 Exhaust.select :close @14:38:51.87 SS.reconfigure :default @14:38:51.92 SS.hold @14:38:52.13 CTV.dial :intake @14:38:52.30 SSV.dial CBV @14:38:52.32 CBV.dial SSV @14:38:53.12 Allowing 130s for 25.0ml of remaining 1000.0ml @14:38:53.14 Intake.select :open @14:38:55.30 Intake @15.3psia, vacuum from 9.8 to 12.8psi @14:38:55.32 SS.reconfigure :inhaling 2.20ml/s @14:38:55.36 SS.seek 25ml @14:39:03.17 Vacuum too high -- waiting up to 40 seconds @14:39:03.19 SS.reconfigure :exhale @14:39:03.24 SS.hold @14:39:11.08 Intake @17.8psia, vacuum from 10.0 to 13.0psi @14:39:11.09 SS.reconfigure :inhaling 1.76ml/s @14:39:11.14 SS.seek 25ml @14:39:14.48 Vacuum too high -- waiting up to 40 seconds @14:39:14.50 SS.reconfigure :exhale @14:39:14.55 SS.hold @14:39:17.80 Intake @17.7psia, vacuum from 10.0 to 13.0psi @14:39:17.82 SS.reconfigure :inhaling 1.41ml/s @14:39:17.87 SS.seek 25ml @14:39:21.33 Vacuum too high -- waiting up to 40 seconds @14:39:21.34 SS.reconfigure :exhale @14:39:21.40 SS.hold @14:39:24.53 Intake @17.9psia, vacuum from 10.0 to 13.0psi @14:39:24.55 SS.reconfigure :inhaling 1.13ml/s @14:39:24.59 SS.seek 25ml @14:39:28.52 Vacuum too high -- waiting up to 40 seconds @14:39:28.53 SS.reconfigure :exhale @14:39:28.59 SS.hold @14:39:31.59 Intake @16.7psia, vacuum from 10.0 to 13.0psi @14:39:31.61 SS.reconfigure :inhaling 0.91ml/s @14:39:31.66 SS.seek 25ml @14:39:35.08 Stroke completed -- waiting for pressure to equalize ... @14:39:35.10 SS.reconfigure :exhale @14:39:35.14 SS.hold @14:40:07.93 Intake.select :close @14:40:08.09 SSV.dial :exhaust @14:40:08.88 Exhaust.select :open @14:40:08.99 SS.seek 0ml @14:40:18.05 Exhaust.select :close @14:40:18.16 SSV.dial CBV @14:40:18.18 CBV.dial SSV @14:40:18.98 Allowing 130s for 25.0ml of remaining 975.0ml @14:40:18.99 Intake.select :open @14:40:21.16 Intake @15.8psia, vacuum from 10.0 to 13.0psi @14:40:21.18 SS.reconfigure :inhaling 0.91ml/s @14:40:21.22 SS.seek 25ml @14:40:50.77 Stroke completed -- waiting for pressure to equalize ... @14:40:50.78 SS.reconfigure :exhale @14:40:50.83 SS.hold @14:41:31.01 Intake.select :close @14:41:31.17 SSV.dial :exhaust @14:41:31.93 Exhaust.select :open @14:41:32.05 SS.seek 0ml @14:41:40.89 Exhaust.select :close @14:41:41.00 SSV.dial CBV @14:41:41.03 CBV.dial SSV @14:41:41.80 Allowing 130s for 25.0ml of remaining 950.0ml @14:41:41.82 Intake.select :open @14:41:43.99 Intake @15.8psia, vacuum from 10.0 to 13.0psi @14:41:44.01 SS.reconfigure :inhaling 0.91ml/s @14:41:44.06 SS.seek 25ml @14:41:47.09 recoverPRVreagent08 @14:41:47.10 PTV.dial :mixing @14:41:47.86 PBV.dial :waste1 @14:41:48.25 delay 30 seconds @14:42:14.16 Stroke completed -- waiting for pressure to equalize ... @14:42:14.17 SS.reconfigure :exhale @14:42:14.22 SS.hold @14:42:18.27 PBV.dial between PSV and waste1 @14:42:18.64 PSV.dial :air @14:42:19.26 PS.reconfigure :fast @14:42:19.31 PS.seek 2.5ml @14:42:43.18 PSV.dial :mixloop @14:42:43.64 delay 2 seconds @14:42:45.65 PSV.dial :air @14:42:46.25 delay 2 seconds @14:42:48.27 PSV.dial :mixloop @14:42:48.72 delay 2 seconds @14:42:49.48 Intake.select :close @14:42:49.64 SSV.dial :exhaust @14:42:50.41 Exhaust.select :open @14:42:50.53 SS.seek 0ml @14:42:50.73 PSV.dial :air @14:42:51.21 PS.seek 0ml @14:42:59.39 Exhaust.select :close @14:42:59.50 SSV.dial CBV @14:42:59.54 CBV.dial SSV @14:43:00.30 Allowing 130s for 25.0ml of remaining 925.0ml @14:43:00.32 Intake.select :open @14:43:02.48 Intake @15.9psia, vacuum from 10.0 to 13.0psi @14:43:02.50 SS.reconfigure :inhaling 0.91ml/s @14:43:02.55 SS.seek 25ml @14:43:15.00 washCycleDA #1 of 2 @14:43:15.01 PSR.select :air @14:43:15.03 PSV.dial PSR @14:43:16.37 Pulling 0.5ml of PSR.wash @14:43:16.38 PSR.select :wash @14:43:16.50 PS.reconfigure :default @14:43:16.55 PS.seek 0.5ml @14:43:22.47 delay 5 seconds @14:43:27.49 PSR.select :air @14:43:27.60 PS.seek 2ml @14:43:32.42 Stroke completed -- waiting for pressure to equalize ... @14:43:32.44 SS.reconfigure :exhale @14:43:32.48 SS.hold @14:43:43.99 PTV.dial :puck @14:43:44.76 PSV.dial PTV @14:43:45.64 PBV.dial :waste1 @14:43:46.07 PS.seek 1.5ml @14:43:52.03 delay 3 seconds @14:43:55.04 PBV.dial between PSV and waste1 @14:43:55.42 PS.seek 0ml @14:44:05.97 Intake.select :close @14:44:06.13 SSV.dial :exhaust @14:44:06.91 Exhaust.select :open @14:44:07.03 SS.seek 0ml @14:44:11.59 delay 2:00 @14:44:15.85 Exhaust.select :close @14:44:15.96 SSV.dial CBV @14:44:15.99 CBV.dial SSV @14:44:16.75 Allowing 130s for 25.0ml of remaining 900.0ml @14:44:16.77 Intake.select :open @14:44:18.93 Intake @15.9psia, vacuum from 10.0 to 13.0psi @14:44:18.95 SS.reconfigure :inhaling 0.91ml/s @14:44:19.00 SS.seek 25ml @14:44:48.95 Vacuum too high -- waiting up to 40 seconds @14:44:48.96 SS.reconfigure :exhale @14:44:49.02 SS.hold @14:44:58.02 Intake @17.4psia, vacuum from 10.0 to 13.0psi @14:44:58.04 SS.reconfigure :inhaling 0.73ml/s @14:44:58.09 SS.seek 25ml @14:44:58.55 Stroke completed -- waiting for pressure to equalize ... @14:44:58.56 SS.reconfigure :exhale @14:44:58.61 SS.hold @14:45:21.06 Intake.select :close @14:45:21.22 SSV.dial :exhaust @14:45:22.46 Exhaust.select :open @14:45:22.58 SS.seek 0ml @14:45:31.38 Exhaust.select :close @14:45:31.49 SSV.dial CBV @14:45:31.51 CBV.dial SSV @14:45:32.30 Allowing 130s for 25.0ml of remaining 875.0ml @14:45:32.31 Intake.select :open @14:45:34.48 Intake @15.9psia, vacuum from 10.0 to 13.0psi @14:45:34.50 SS.reconfigure :inhaling 0.73ml/s @14:45:34.55 SS.seek 25ml @14:46:10.90 Stroke completed -- waiting for pressure to equalize ... @14:46:10.92 SS.reconfigure :exhale @14:46:10.97 SS.hold @14:46:11.61 recoverPSRreagent08 @14:46:11.62 PTV.dial :puck @14:46:11.85 PBV.dial :waste1 @14:46:12.25 delay 30 seconds @14:46:37.66 Intake.select :close @14:46:37.82 SSV.dial :exhaust @14:46:38.58 Exhaust.select :open @14:46:38.70 SS.seek 0ml @14:46:42.27 PBV.dial between PSV and waste1 @14:46:42.63 PSR.select :air @14:46:42.65 PSV.dial PSR @14:46:43.42 PS.reconfigure :fast @14:46:43.47 PS.seek 2.5ml @14:46:47.69 Exhaust.select :close @14:46:47.80 SSV.dial CBV @14:46:47.82 CBV.dial SSV @14:46:48.59 Allowing 130s for 25.0ml of remaining 850.0ml @14:46:48.61 Intake.select :open @14:46:50.78 Intake @15.9psia, vacuum from 10.0 to 13.0psi @14:46:50.80 SS.reconfigure :inhaling 0.73ml/s @14:46:50.84 SS.seek 25ml @14:47:07.43 PSV.dial PTV @14:47:08.20 delay 5 seconds @14:47:13.22 PSV.dial PSR @14:47:13.98 delay 5 seconds @14:47:19.00 PSV.dial PTV @14:47:19.88 delay 5 seconds @14:47:24.91 PSV.dial :air @14:47:25.69 PS.seek 0ml @14:47:26.82 Stroke completed -- waiting for pressure to equalize ... @14:47:26.84 SS.reconfigure :exhale @14:47:26.89 SS.hold @14:47:49.05 washCycleDA #2 of 2 @14:47:49.06 PSR.select :air @14:47:49.08 PSV.dial PSR @14:47:50.41 Pulling 0.5ml of PSR.wash @14:47:50.42 PSR.select :wash @14:47:50.54 PS.reconfigure :default @14:47:50.59 PS.seek 0.5ml @14:47:51.92 Intake.select :close @14:47:52.08 SSV.dial :exhaust @14:47:52.85 Exhaust.select :open @14:47:52.97 SS.seek 0ml @14:47:56.52 delay 5 seconds @14:48:01.54 PSR.select :air @14:48:01.66 PS.seek 2ml @14:48:01.79 Exhaust.select :close @14:48:01.91 SSV.dial CBV @14:48:01.93 CBV.dial SSV @14:48:02.70 Allowing 130s for 25.0ml of remaining 825.0ml @14:48:02.72 Intake.select :open @14:48:04.88 Intake @15.9psia, vacuum from 10.0 to 13.0psi @14:48:05.28 SS.reconfigure :inhaling 0.73ml/s @14:48:05.40 SS.seek 25ml @14:48:17.87 PTV.dial :puck @14:48:17.94 PSV.dial PTV @14:48:18.69 PBV.dial :waste1 @14:48:19.13 PS.seek 1.5ml @14:48:25.12 delay 3 seconds @14:48:28.14 PBV.dial between PSV and waste1 @14:48:28.51 PS.seek 0ml @14:48:41.78 Stroke completed -- waiting for pressure to equalize ... @14:48:41.79 SS.reconfigure :exhale @14:48:41.84 SS.hold @14:48:44.68 delay 2:00 @14:49:08.42 Intake.select :close @14:49:08.58 SSV.dial :exhaust @14:49:09.35 Exhaust.select :open @14:49:09.47 SS.seek 0ml @14:49:18.56 Exhaust.select :close @14:49:18.67 SSV.dial CBV @14:49:18.70 CBV.dial SSV @14:49:19.48 Allowing 130s for 25.0ml of remaining 800.0ml @14:49:19.50 Intake.select :open @14:49:21.66 Intake @15.8psia, vacuum from 10.0 to 13.0psi @14:49:21.68 SS.reconfigure :inhaling 0.73ml/s @14:49:21.73 SS.seek 25ml @14:49:57.42 Stroke completed -- waiting for pressure to equalize ... @14:49:57.43 SS.reconfigure :exhale @14:49:57.48 SS.hold @14:50:22.42 Intake.select :close @14:50:22.59 SSV.dial :exhaust @14:50:23.35 Exhaust.select :open @14:50:23.47 SS.seek 0ml @14:50:32.43 Exhaust.select :close @14:50:32.55 SSV.dial CBV @14:50:32.57 CBV.dial SSV @14:50:33.34 Allowing 130s for 25.0ml of remaining 775.0ml @14:50:33.36 Intake.select :open @14:50:35.52 Intake @16.0psia, vacuum from 10.0 to 13.0psi @14:50:35.54 SS.reconfigure :inhaling 0.73ml/s @14:50:35.59 SS.seek 25ml @14:50:44.70 recoverPSRreagent08 @14:50:44.72 PTV.dial :puck @14:50:44.79 PBV.dial :waste1 @14:50:45.16 delay 30 seconds @14:51:11.37 Stroke completed -- waiting for pressure to equalize ... @14:51:11.38 SS.reconfigure :exhale @14:51:11.43 SS.hold @14:51:15.18 PBV.dial between PSV and waste1 @14:51:15.61 PSR.select :air @14:51:15.63 PSV.dial PSR @14:51:17.18 PS.reconfigure :fast @14:51:17.23 PS.seek 2.5ml @14:51:35.99 Intake.select :close @14:51:36.15 SSV.dial :exhaust @14:51:36.93 Exhaust.select :open @14:51:37.05 SS.seek 0ml @14:51:41.24 PSV.dial PTV @14:51:42.00 delay 5 seconds @14:51:46.04 Exhaust.select :close @14:51:46.15 SSV.dial CBV @14:51:46.19 CBV.dial SSV @14:51:46.95 Allowing 130s for 25.0ml of remaining 750.0ml @14:51:46.96 Intake.select :open @14:51:47.02 PSV.dial PSR @14:51:47.79 delay 5 seconds @14:51:49.13 Intake @15.9psia, vacuum from 10.0 to 13.0psi @14:51:49.15 SS.reconfigure :inhaling 0.73ml/s @14:51:49.20 SS.seek 25ml @14:51:52.80 PSV.dial PTV @14:51:53.71 delay 5 seconds @14:51:58.73 PSV.dial :air @14:51:59.51 PS.seek 0ml @14:52:22.86 getPMLReagent08 @14:52:22.87 PRV.dial :air @14:52:23.33 delay 5 seconds @14:52:24.54 Vacuum too high -- waiting up to 40 seconds @14:52:24.56 SS.reconfigure :exhale @14:52:24.61 SS.hold @14:52:28.35 PTV.dial :PRVmixing @14:52:29.67 delay 5 seconds @14:52:33.30 Intake @16.2psia, vacuum from 10.0 to 13.0psi @14:52:33.32 SS.reconfigure :inhaling 0.59ml/s @14:52:33.37 SS.seek 25ml @14:52:34.69 PRV.dial :ab2 @14:52:34.88 Stroke completed -- waiting for pressure to equalize ... @14:52:34.89 SS.reconfigure :exhale @14:52:34.94 SS.hold @14:52:35.14 PTV.dial :PRVmixing @14:52:35.24 PSV.dial :mixloop @14:52:36.13 PS.reconfigure :default @14:52:36.25 PS.seek 0.3ml @14:52:40.24 delay 2 seconds @14:52:42.25 PRV.dial :air @14:52:42.78 PS.seek 0.55ml @14:52:46.33 addPMLReagent08 @14:52:46.34 PSV.dial :air @14:52:46.80 PS.seek 2ml @14:52:49.90 Intake.select :close @14:52:50.06 SSV.dial :exhaust @14:52:50.82 Exhaust.select :open @14:52:50.94 SS.seek 0ml @14:52:59.93 Exhaust.select :close @14:53:00.04 SSV.dial CBV @14:53:00.07 CBV.dial SSV @14:53:00.84 Allowing 130s for 25.0ml of remaining 725.0ml @14:53:00.85 Intake.select :open @14:53:02.45 PSV.dial :PML @14:53:02.92 PTV.dial :mixing @14:53:03.49 Intake @16.0psia, vacuum from 10.0 to 13.0psi @14:53:03.51 SS.reconfigure :inhaling 0.59ml/s @14:53:03.56 SS.seek 25ml @14:53:03.69 PBV.dial :waste1 @14:53:04.13 PS.seek 1.85ml @14:53:06.49 PBV.dial between PSV and waste1 @14:53:06.87 PS.seek 0ml @14:53:26.61 delay 2 seconds @14:53:28.63 delay 8:00 @14:53:47.82 Stroke completed -- waiting for pressure to equalize ... @14:53:47.83 SS.reconfigure :exhale @14:53:47.88 SS.hold @14:54:08.73 Intake.select :close @14:54:08.89 SSV.dial :exhaust @14:54:09.67 Exhaust.select :open @14:54:09.79 SS.seek 0ml @14:54:18.62 Exhaust.select :close @14:54:18.72 SSV.dial CBV @14:54:18.74 CBV.dial SSV @14:54:19.52 Allowing 130s for 25.0ml of remaining 700.0ml @14:54:19.54 Intake.select :open @14:54:21.70 Intake @16.0psia, vacuum from 10.0 to 13.0psi @14:54:21.72 SS.reconfigure :inhaling 0.59ml/s @14:54:21.77 SS.seek 25ml @14:55:06.47 Stroke completed -- waiting for pressure to equalize ... @14:55:06.48 SS.reconfigure :exhale @14:55:06.53 SS.hold @14:55:27.06 Intake.select :close @14:55:27.21 SSV.dial :exhaust @14:55:27.98 Exhaust.select :open @14:55:28.10 SS.seek 0ml @14:55:36.93 Exhaust.select :close @14:55:37.04 SSV.dial CBV @14:55:37.06 CBV.dial SSV @14:55:37.83 Allowing 130s for 25.0ml of remaining 675.0ml @14:55:37.85 Intake.select :open @14:55:40.02 Intake @16.0psia, vacuum from 10.0 to 13.0psi @14:55:40.04 SS.reconfigure :inhaling 0.59ml/s @14:55:40.08 SS.seek 25ml @14:56:24.81 Stroke completed -- waiting for pressure to equalize ... @14:56:24.83 SS.reconfigure :exhale @14:56:24.88 SS.hold @14:56:47.33 Intake.select :close @14:56:47.49 SSV.dial :exhaust @14:56:48.27 Exhaust.select :open @14:56:48.39 SS.seek 0ml @14:56:57.44 Exhaust.select :close @14:56:57.55 SSV.dial CBV @14:56:57.59 CBV.dial SSV @14:56:58.35 Allowing 130s for 25.0ml of remaining 650.0ml @14:56:58.36 Intake.select :open @14:57:00.53 Intake @16.0psia, vacuum from 10.0 to 13.0psi @14:57:00.55 SS.reconfigure :inhaling 0.59ml/s @14:57:00.60 SS.seek 25ml @14:57:44.94 Stroke completed -- waiting for pressure to equalize ... @14:57:44.95 SS.reconfigure :exhale @14:57:45.00 SS.hold @14:58:11.54 Intake.select :close @14:58:11.70 SSV.dial :exhaust @14:58:12.48 Exhaust.select :open @14:58:12.60 SS.seek 0ml @14:58:21.66 Exhaust.select :close @14:58:21.77 SSV.dial CBV @14:58:21.79 CBV.dial SSV @14:58:22.56 Allowing 130s for 25.0ml of remaining 625.0ml @14:58:22.58 Intake.select :open @14:58:24.75 Intake @16.0psia, vacuum from 10.0 to 13.0psi @14:58:24.77 SS.reconfigure :inhaling 0.59ml/s @14:58:24.81 SS.seek 25ml @14:59:05.49 Vacuum too high -- waiting up to 40 seconds @14:59:05.51 SS.reconfigure :exhale @14:59:05.56 SS.hold @14:59:15.92 Intake @17.0psia, vacuum from 10.0 to 13.0psi @14:59:15.94 SS.reconfigure :inhaling 0.47ml/s @14:59:15.98 SS.seek 25ml @14:59:20.40 Stroke completed -- waiting for pressure to equalize ... @14:59:20.42 SS.reconfigure :exhale @14:59:20.46 SS.hold @14:59:36.78 Intake.select :close @14:59:36.94 SSV.dial :exhaust @14:59:37.70 Exhaust.select :open @14:59:37.82 SS.seek 0ml @14:59:46.66 Exhaust.select :close @14:59:46.77 SSV.dial CBV @14:59:46.79 CBV.dial SSV @14:59:47.56 Allowing 130s for 25.0ml of remaining 600.0ml @14:59:47.58 Intake.select :open @14:59:49.79 Intake @15.9psia, vacuum from 10.0 to 13.0psi @14:59:49.81 SS.reconfigure :inhaling 0.47ml/s @14:59:49.86 SS.seek 25ml @15:00:45.50 Stroke completed -- waiting for pressure to equalize ... @15:00:45.51 SS.reconfigure :exhale @15:00:45.56 SS.hold @15:01:06.41 Intake.select :close @15:01:06.57 SSV.dial :exhaust @15:01:07.33 Exhaust.select :open @15:01:07.45 SS.seek 0ml @15:01:16.54 Exhaust.select :close @15:01:16.65 SSV.dial CBV @15:01:16.70 CBV.dial SSV @15:01:17.44 Allowing 130s for 25.0ml of remaining 575.0ml @15:01:17.45 Intake.select :open @15:01:19.62 Intake @16.0psia, vacuum from 10.0 to 13.0psi @15:01:19.64 SS.reconfigure :inhaling 0.47ml/s @15:01:19.69 SS.seek 25ml @15:01:28.65 recoverPRVreagent08 @15:01:28.66 PTV.dial :mixing @15:01:28.74 PBV.dial :waste1 @15:01:29.12 delay 30 seconds @15:01:59.14 PBV.dial between PSV and waste1 @15:01:59.51 PSV.dial :air @15:01:59.98 PS.reconfigure :fast @15:02:00.03 PS.seek 2.5ml @15:02:14.90 Stroke completed -- waiting for pressure to equalize ... @15:02:14.91 SS.reconfigure :exhale @15:02:14.96 SS.hold @15:02:23.94 PSV.dial :mixloop @15:02:24.41 delay 2 seconds @15:02:26.43 PSV.dial :air @15:02:26.90 delay 2 seconds @15:02:28.92 PSV.dial :mixloop @15:02:29.38 delay 2 seconds @15:02:31.47 PSV.dial :air @15:02:31.93 PS.seek 0ml @15:02:37.60 Intake.select :close @15:02:37.76 SSV.dial :exhaust @15:02:38.53 Exhaust.select :open @15:02:38.65 SS.seek 0ml @15:02:47.46 Exhaust.select :close @15:02:47.57 SSV.dial CBV @15:02:47.59 CBV.dial SSV @15:02:48.37 Allowing 130s for 25.0ml of remaining 550.0ml @15:02:48.39 Intake.select :open @15:02:50.97 Intake @16.1psia, vacuum from 10.0 to 13.0psi @15:02:50.99 SS.reconfigure :inhaling 0.47ml/s @15:02:51.03 SS.seek 25ml @15:02:55.48 washCycleDA #1 of 3 @15:02:55.49 PSR.select :air @15:02:55.51 PSV.dial PSR @15:02:56.85 Pulling 0.5ml of PSR.wash @15:02:56.86 PSR.select :wash @15:02:56.98 PS.reconfigure :default @15:02:57.03 PS.seek 0.5ml @15:03:03.01 delay 5 seconds @15:03:08.03 PSR.select :air @15:03:08.15 PS.seek 2ml @15:03:24.41 PTV.dial :puck @15:03:25.16 PSV.dial PTV @15:03:25.91 PBV.dial :waste1 @15:03:26.35 PS.seek 1.5ml @15:03:32.29 delay 3 seconds @15:03:35.31 PBV.dial between PSV and waste1 @15:03:35.68 PS.seek 0ml @15:03:46.32 Stroke completed -- waiting for pressure to equalize ... @15:03:46.33 SS.reconfigure :exhale @15:03:46.38 SS.hold @15:03:51.80 delay 2:00 @15:04:13.03 Intake.select :close @15:04:13.19 SSV.dial :exhaust @15:04:13.96 Exhaust.select :open @15:04:14.08 SS.seek 0ml @15:04:23.13 Exhaust.select :close @15:04:23.24 SSV.dial CBV @15:04:23.29 CBV.dial SSV @15:04:24.04 Allowing 130s for 25.0ml of remaining 525.0ml @15:04:24.06 Intake.select :open @15:04:26.23 Intake @16.2psia, vacuum from 10.0 to 13.0psi @15:04:26.24 SS.reconfigure :inhaling 0.47ml/s @15:04:26.29 SS.seek 25ml @15:05:21.32 Stroke completed -- waiting for pressure to equalize ... @15:05:21.33 SS.reconfigure :exhale @15:05:21.38 SS.hold @15:05:50.05 Intake.select :close @15:05:50.21 SSV.dial :exhaust @15:05:50.96 Exhaust.select :open @15:05:51.08 SS.seek 0ml @15:05:51.82 recoverPSRreagent08 @15:05:51.83 PTV.dial :puck @15:05:52.03 PBV.dial :waste1 @15:05:52.41 delay 30 seconds @15:06:00.07 Exhaust.select :close @15:06:00.18 SSV.dial CBV @15:06:00.21 CBV.dial SSV @15:06:00.98 Allowing 130s for 25.0ml of remaining 500.0ml @15:06:00.99 Intake.select :open @15:06:03.16 Intake @16.2psia, vacuum from 10.0 to 13.0psi @15:06:03.18 SS.reconfigure :inhaling 0.47ml/s @15:06:03.23 SS.seek 25ml @15:06:22.43 PBV.dial between PSV and waste1 @15:06:22.81 PSR.select :air @15:06:22.83 PSV.dial PSR @15:06:23.60 PS.reconfigure :fast @15:06:23.65 PS.seek 2.5ml @15:06:48.01 PSV.dial PTV @15:06:49.02 delay 5 seconds @15:06:54.04 PSV.dial PSR @15:06:54.79 delay 5 seconds @15:06:57.08 Vacuum too high -- waiting up to 40 seconds @15:06:57.10 SS.reconfigure :exhale @15:06:57.15 SS.hold @15:06:59.80 PSV.dial PTV @15:07:00.56 delay 5 seconds @15:07:05.58 PSV.dial :air @15:07:06.35 PS.seek 0ml @15:07:13.61 Intake @18.1psia, vacuum from 10.0 to 13.0psi @15:07:13.63 SS.reconfigure :inhaling 0.38ml/s @15:07:13.68 SS.seek 25ml @15:07:15.60 Stroke completed -- waiting for pressure to equalize ... @15:07:15.61 SS.reconfigure :exhale @15:07:15.66 SS.hold @15:07:29.66 washCycleDA #2 of 3 @15:07:29.67 PSR.select :air @15:07:29.69 PSV.dial PSR @15:07:31.02 Pulling 0.5ml of PSR.wash @15:07:31.03 PSR.select :wash @15:07:31.14 PS.reconfigure :default @15:07:31.19 PS.seek 0.5ml @15:07:32.29 Intake.select :close @15:07:32.46 SSV.dial :exhaust @15:07:33.23 Exhaust.select :open @15:07:33.34 SS.seek 0ml @15:07:37.06 delay 5 seconds @15:07:42.08 PSR.select :air @15:07:42.20 PS.seek 2ml @15:07:42.35 Exhaust.select :close @15:07:42.94 SSV.dial CBV @15:07:42.96 CBV.dial SSV @15:07:43.74 Allowing 130s for 25.0ml of remaining 475.0ml @15:07:43.76 Intake.select :open @15:07:45.92 Intake @16.2psia, vacuum from 10.0 to 13.0psi @15:07:45.94 SS.reconfigure :inhaling 0.38ml/s @15:07:45.99 SS.seek 25ml @15:07:58.34 PTV.dial :puck @15:07:58.42 PSV.dial PTV @15:07:59.17 PBV.dial :waste1 @15:07:59.61 PS.seek 1.5ml @15:08:05.53 delay 3 seconds @15:08:08.55 PBV.dial between PSV and waste1 @15:08:08.93 PS.seek 0ml @15:08:25.16 delay 2:00 @15:08:54.22 Stroke completed -- waiting for pressure to equalize ... @15:08:54.24 SS.reconfigure :exhale @15:08:54.28 SS.hold @15:09:20.85 Intake.select :close @15:09:21.01 SSV.dial :exhaust @15:09:21.79 Exhaust.select :open @15:09:21.91 SS.seek 0ml @15:09:30.71 Exhaust.select :close @15:09:30.83 SSV.dial CBV @15:09:30.87 CBV.dial SSV @15:09:31.63 Allowing 130s for 25.0ml of remaining 450.0ml @15:09:31.65 Intake.select :open @15:09:33.81 Intake @16.1psia, vacuum from 10.0 to 13.0psi @15:09:33.83 SS.reconfigure :inhaling 0.38ml/s @15:09:33.88 SS.seek 25ml @15:10:25.18 recoverPSRreagent08 @15:10:25.20 PTV.dial :puck @15:10:25.27 PBV.dial :waste1 @15:10:25.64 delay 30 seconds @15:10:42.44 Stroke completed -- waiting for pressure to equalize ... @15:10:42.45 SS.reconfigure :exhale @15:10:42.50 SS.hold @15:10:55.66 PBV.dial between PSV and waste1 @15:10:56.03 PSR.select :air @15:10:56.05 PSV.dial PSR @15:10:56.82 PS.reconfigure :fast @15:10:56.88 PS.seek 2.5ml @15:11:13.30 Intake.select :close @15:11:13.46 SSV.dial :exhaust @15:11:14.21 Exhaust.select :open @15:11:14.32 SS.seek 0ml @15:11:20.84 PSV.dial PTV @15:11:21.61 delay 5 seconds @15:11:23.22 Exhaust.select :close @15:11:23.33 SSV.dial CBV @15:11:23.35 CBV.dial SSV @15:11:24.13 Allowing 130s for 25.0ml of remaining 425.0ml @15:11:24.14 Intake.select :open @15:11:26.31 Intake @16.1psia, vacuum from 10.0 to 13.0psi @15:11:26.33 SS.reconfigure :inhaling 0.38ml/s @15:11:26.38 SS.seek 25ml @15:11:26.90 PSV.dial PSR @15:11:27.66 delay 5 seconds @15:11:32.68 PSV.dial PTV @15:11:33.44 delay 5 seconds @15:11:38.46 PSV.dial :air @15:11:39.22 PS.seek 0ml @15:12:02.50 washCycleDA #3 of 3 @15:12:02.51 PSR.select :air @15:12:02.53 PSV.dial PSR @15:12:03.87 Pulling 0.5ml of PSR.wash @15:12:03.88 PSR.select :wash @15:12:04.00 PS.reconfigure :default @15:12:04.05 PS.seek 0.5ml @15:12:10.00 delay 5 seconds @15:12:15.02 PSR.select :air @15:12:15.14 PS.seek 2ml @15:12:31.25 PTV.dial :puck @15:12:31.32 PSV.dial PTV @15:12:32.09 PBV.dial :waste1 @15:12:32.52 PS.seek 1.5ml @15:12:35.36 Stroke completed -- waiting for pressure to equalize ... @15:12:35.37 SS.reconfigure :exhale @15:12:35.42 SS.hold @15:12:38.43 delay 3 seconds @15:12:41.45 PBV.dial between PSV and waste1 @15:12:41.87 PS.seek 0ml @15:12:58.10 delay 2:00 @15:13:09.40 Intake.select :close @15:13:09.56 SSV.dial :exhaust @15:13:10.35 Exhaust.select :open @15:13:10.46 SS.seek 0ml @15:13:19.36 Exhaust.select :close @15:13:19.47 SSV.dial CBV @15:13:19.51 CBV.dial SSV @15:13:20.28 Allowing 130s for 25.0ml of remaining 400.0ml @15:13:20.30 Intake.select :open @15:13:22.46 Intake @16.2psia, vacuum from 10.0 to 13.0psi @15:13:22.48 SS.reconfigure :inhaling 0.38ml/s @15:13:22.53 SS.seek 25ml @15:14:27.60 Vacuum too high -- waiting up to 40 seconds @15:14:27.62 SS.reconfigure :exhale @15:14:27.67 SS.hold @15:14:44.27 Intake @17.0psia, vacuum from 10.0 to 13.0psi @15:14:44.29 SS.reconfigure :inhaling 0.31ml/s @15:14:44.34 SS.seek 25ml @15:14:48.58 Stroke completed -- waiting for pressure to equalize ... @15:14:48.59 SS.reconfigure :exhale @15:14:48.64 SS.hold @15:14:58.12 recoverPSRreagent08 @15:14:58.13 PTV.dial :puck @15:14:58.20 PBV.dial :waste1 @15:14:58.57 delay 30 seconds @15:15:11.74 Intake.select :close @15:15:11.90 SSV.dial :exhaust @15:15:12.66 Exhaust.select :open @15:15:12.77 SS.seek 0ml @15:15:21.79 Exhaust.select :close @15:15:21.90 SSV.dial CBV @15:15:21.95 CBV.dial SSV @15:15:22.70 Allowing 130s for 25.0ml of remaining 375.0ml @15:15:22.72 Intake.select :open @15:15:24.88 Intake @16.2psia, vacuum from 10.0 to 13.0psi @15:15:24.90 SS.reconfigure :inhaling 0.31ml/s @15:15:24.95 SS.seek 25ml @15:15:28.59 PBV.dial between PSV and waste1 @15:15:28.95 PSR.select :air @15:15:28.97 PSV.dial PSR @15:15:29.73 PS.reconfigure :fast @15:15:29.78 PS.seek 2.5ml @15:15:53.65 PSV.dial PTV @15:15:54.42 delay 5 seconds @15:15:59.44 PSV.dial PSR @15:16:00.20 delay 5 seconds @15:16:05.22 PSV.dial PTV @15:16:05.98 delay 5 seconds @15:16:11.00 PSV.dial :air @15:16:11.77 PS.seek 0ml @15:16:35.76 Gate.power :camera,:ON @15:16:35.77 Mixing DA Substrates @15:16:35.78 PSR.select :air @15:16:35.80 PSV.dial PSR @15:16:37.19 Pulling 0.25ml of PSR.sub1 @15:16:37.22 PSR.select :sub1 @15:16:37.43 PS.reconfigure :default @15:16:37.55 PS.seek 0.25ml @15:16:41.18 delay 5 seconds @15:16:46.20 Pulling 0.25ml of PSR.sub2 @15:16:46.22 PSR.select :sub2 @15:16:46.33 PS.seek 0.5ml @15:16:49.74 delay 5 seconds @15:16:50.87 Stroke completed -- waiting for pressure to equalize ... @15:16:50.88 SS.reconfigure :exhale @15:16:50.93 SS.hold @15:16:54.76 PSR.select :air @15:16:54.87 PS.seek 2ml @15:17:11.02 PTV.dial :puck @15:17:11.10 PSV.dial PTV @15:17:11.87 PBV.dial :waste1 @15:17:12.31 PS.seek 1.5ml @15:17:18.27 delay 3 seconds @15:17:21.29 PBV.dial between PSV and waste1 @15:17:21.60 Intake.select :close @15:17:21.67 PS.seek 0ml @15:17:21.78 SSV.dial :exhaust @15:17:22.57 Exhaust.select :open @15:17:22.69 SS.seek 0ml @15:17:31.75 Exhaust.select :close @15:17:31.86 SSV.dial CBV @15:17:31.88 CBV.dial SSV @15:17:32.65 Allowing 130s for 25.0ml of remaining 350.0ml @15:17:32.67 Intake.select :open @15:17:34.84 Intake @16.2psia, vacuum from 10.0 to 13.0psi @15:17:34.86 SS.reconfigure :inhaling 0.31ml/s @15:17:34.90 SS.seek 25ml @15:17:37.87 delay 10 seconds @15:17:47.90 recoverPSRreagent08 PTV.dial :puck @15:17:47.98 PBV.dial :waste1 @15:17:48.37 delay 30 seconds @15:18:18.39 PBV.dial between PSV and waste1 @15:18:18.77 PSR.select :air @15:18:18.79 PSV.dial PSR @15:18:19.58 PS.reconfigure :fast @15:18:19.63 PS.seek 2.5ml @15:18:43.93 PSV.dial PTV @15:18:44.84 delay 5 seconds @15:18:49.86 PSV.dial PSR @15:18:50.63 delay 5 seconds @15:18:55.65 PSV.dial PTV @15:18:56.44 delay 5 seconds @15:19:01.38 Stroke completed -- waiting for pressure to equalize ... @15:19:01.40 SS.reconfigure :exhale @15:19:01.45 SS.hold @15:19:01.49 PSV.dial :air @15:19:02.55 PS.seek 0ml @15:19:26.46 Additional air clear @15:19:26.47 PSR.select :air @15:19:26.49 PSV.dial PSR @15:19:27.99 Pulling 2ml of PSR.air @15:19:28.00 PSR.select :air @15:19:28.02 PS.reconfigure :default @15:19:28.07 PS.seek 2ml @15:19:34.51 Intake.select :close @15:19:34.67 SSV.dial :exhaust @15:19:35.46 Exhaust.select :open @15:19:35.58 SS.seek 0ml @15:19:44.49 Exhaust.select :close @15:19:44.60 SSV.dial CBV @15:19:44.64 CBV.dial SSV @15:19:45.42 Allowing 130s for 25.0ml of remaining 325.0ml @15:19:45.43 Intake.select :open @15:19:47.60 Intake @16.4psia, vacuum from 10.0 to 13.0psi @15:19:47.62 SS.reconfigure :inhaling 0.31ml/s @15:19:47.67 SS.seek 25ml @15:19:49.30 delay 5 seconds @15:19:54.32 PTV.dial :puck @15:19:54.40 PSV.dial PTV @15:19:55.15 PBV.dial :waste1 @15:19:55.58 PS.seek 1.5ml @15:20:01.58 delay 3 seconds @15:20:04.60 PBV.dial between PSV and waste1 @15:20:04.98 delay 1 second @15:20:05.99 PSV.dial :waste1 @15:20:07.07 PS.seek 0ml @15:20:23.35 Processing Puck Heater at 28C before imaging @15:20:23.36 PH.coast @15:20:23.47 Imaging da puck in Processing Clamp while storing it to tube 4 @15:20:23.64 SC.reconfigure :tight @15:20:23.69 SC.seek 4 @15:20:27.23 SC.configure :limp @15:20:27.45 Elbow.reconfigure :tight @15:20:27.50 Elbow.seek 4 @15:20:29.27 Forearm.reconfigure :tight @15:20:29.33 Forearm.seek 4 + 450 counts @15:20:30.36 SE.reconfigure :pick @15:20:30.42 SE.seek :beamBreak @15:20:31.57 SE.configure :default @15:20:31.73 Elbow.reconfigure :limp @15:20:31.78 Forearm.reconfigure :loose @15:20:31.82 Forearm.seek 4 @15:20:32.50 Elbow.hold @15:20:32.63 Elbow.reconfigure :tight @15:20:32.73 SE.seek 30.09 Pucks below Top Plate @15:20:33.21 Forearm.force -20 @15:20:33.25 Forearm.seek 4 + 303 counts @15:20:33.54 Forearm.reconfigure :tight @15:20:33.59 Forearm.seek 4 + 450 counts @15:20:33.79 SE.seek 31.09 Pucks below Top Plate @15:20:34.44 Forearm.reconfigure :loose @15:20:34.49 Forearm.seek :retract @15:20:35.41 Elbow.seek PC @15:20:38.09 Elbow.reconfigure :loose @15:20:38.19 Forearm.seek PC @15:20:39.13 Hand.close @15:20:39.79 PC.seek :open @15:20:44.57 Forearm.seek :retract @15:20:45.50 Elbow.reconfigure :tight @15:20:45.55 Elbow.seek 4 @15:20:48.15 Forearm.reconfigure :tight @15:20:48.20 Forearm.seek 4 + 450 counts @15:20:49.17 Hand.open @15:20:49.85 SE.seek 30.74 Pucks below Top Plate @15:20:50.11 SE.reconfigure :seal @15:20:50.16 SE.seek 29.78 Pucks below Top Plate @15:20:50.68 Lights, camera, ACTION! @15:20:50.72 PuckCamera: snap -bin=2,2 -auto /var/log/esp/da23dec0715h1000ml.tif @15:21:10.22 Waiting >20 seconds for da to relinquish FlushPuck @15:21:12.31 SX HX9/16 (USB:20): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 18.316 seconds /var/log/esp/da23dec0715h1000ml.tif: TIFF Upload Complete @15:21:12.34 PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da23dec0715h1000ml15s.tif @15:21:16.70 Stroke completed -- waiting for pressure to equalize ... @15:21:16.71 SS.reconfigure :exhale @15:21:16.77 SS.hold @15:21:27.99 SX HX9/16 (USB:20): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15 seconds /var/log/esp/midres/da23dec0715h1000ml15s.tif: TIFF Upload Complete @15:21:28.01 PuckCamera: snap -bin=2,2 20.0 /var/log/esp/midres/da23dec0715h1000ml20s.tif @15:21:48.63 SX HX9/16 (USB:20): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 20 seconds /var/log/esp/midres/da23dec0715h1000ml20s.tif: TIFF Upload Complete @15:21:48.65 DAfans.email "18.316 sec auto-exposure",Subject: "da23dec0715h1000ml images!" @15:21:48.68 Gate.power :camera,:OFF @15:21:49.09 Elbow.seek 4 @15:21:49.87 Forearm.seek 4 + 450 counts @15:21:50.04 SE.reconfigure :gently @15:21:50.17 SE.seek 30.5 Pucks below Top Plate @15:21:50.95 Elbow.reconfigure :limp @15:21:51.02 Forearm.reconfigure :loose @15:21:51.06 Forearm.seek 4 @15:21:51.35 Elbow.hold @15:21:51.41 Elbow.reconfigure :tight @15:21:51.47 SE.reconfigure :default @15:21:51.51 SE.seek 30.68 Pucks below Top Plate @15:21:52.03 Forearm.force -20 @15:21:52.11 Forearm.seek 4 + 264 counts @15:21:52.41 Forearm.reconfigure :tight @15:21:52.46 Forearm.seek 4 + 450 counts @15:21:52.77 SE.seek :down @15:21:53.43 SE.stop @15:21:53.92 Forearm.reconfigure :loose @15:21:54.00 Forearm.seek :retract @15:21:55.09 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @15:21:55.51 Elbow.seek FlushPuck::Garage @15:21:55.68 SS.reconfigure :default @15:21:55.78 SS.hold @15:21:55.92 Intake.select :close @15:21:56.20 ShallowSampler::Clogged in WCR -- Filtering too Slowly after 700.0ml @15:21:56.23 SSV.dial :exhaust @15:21:57.06 Exhaust.select :open @15:21:57.23 SS.reconfigure :exhale @15:21:58.58 SS.seek 0ml @15:21:58.77 Elbow.reconfigure :loose @15:21:59.02 Forearm.seek :garage @15:22:00.03 Hand.close @15:22:00.93 Forearm.seek :retract @15:22:01.97 Elbow.reconfigure :tight @15:22:02.07 Elbow.seek PC @15:22:03.47 Elbow.reconfigure :loose @15:22:03.67 Forearm.reconfigure :tight @15:22:03.76 Forearm.seek PC @15:22:05.31 PC.seek :closed @15:22:07.73 Exhaust.select :close @15:22:07.99 Sampled 700.0ml @15:22:08.06 Drying puck before fixing for 5:00 @15:22:08.08 CBV.dial :waste2b @15:22:08.96 delay 1:00 @15:22:10.06 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.66 @15:22:10.21 Hand.open @15:22:10.95 Forearm.reconfigure :loose @15:22:11.04 Forearm.seek :retract @15:22:12.02 PSR.select :air @15:22:12.06 PSV.dial PSR @15:22:12.61 Pulling 0.5ml of PSR.flush @15:22:12.64 PSR.select :flush @15:22:12.81 PS.seek 0.5ml @15:22:18.82 delay 5 seconds @15:22:23.50 upsync @15:22:23.85 PSR.select :air @15:22:23.97 PS.seek 2.5ml @15:22:45.20 delay 1 second @15:22:46.22 PRV.dial :waste2,avoiding: :ab2 @15:22:48.11 PTV.dial :PRVmixing @15:22:49.42 PSV.dial :mixloop @15:22:50.47 PS.seek :empty @15:23:08.99 CSV.dial :air @15:23:09.47 CS.reconfigure :fast @15:23:09.52 CS.seek 10ml @15:23:16.80 PSR.select :air @15:23:16.82 PSV.dial PSR @15:23:17.86 Pulling 0.5ml of PSR.flush @15:23:17.87 PSR.select :flush @15:23:17.99 PS.seek 0.5ml @15:23:23.89 delay 5 seconds @15:23:28.91 PSR.select :air @15:23:29.03 PS.seek 2.5ml @15:23:34.31 Pumping wcr puck from tube 5 up to 35psi... @15:23:34.33 CBV.dial SSV @15:23:35.61 CBV.dial between CSV and waste2b @15:23:36.58 CSV.dial between air and CTV @15:23:36.92 CS.seek 7.001ml @15:23:44.50 CTV.dial CSV,avoiding: :air @15:23:44.52 CSV.dial CTV @15:23:45.34 CS.reconfigure :pressurize @15:23:45.39 CS.seek 0ml @15:23:50.36 delay 1 second @15:23:51.38 PBV.dial :waste1 @15:23:51.75 PTV.dial :mixing @15:23:52.50 PSV.dial :mixloop @15:23:53.57 PS.seek :empty @15:23:58.54 CBV.dial :waste2b @15:23:58.89 CS.reconfigure :default @15:23:58.94 CS.seek 0ml @15:24:05.15 delay 1:00 @15:24:19.98 PSV.dial PSR @15:24:21.05 PSR.select :flush @15:24:21.17 PS.seek 1ml @15:24:32.23 PSR.select :air @15:24:32.35 PS.seek 2.5ml @15:24:48.54 PSV.dial PTV @15:24:49.31 PTV.dial :puck @15:24:50.06 PBV.dial :waste1 @15:24:50.24 PS.seek 0ml @15:25:05.17 CTV.dial :air,avoiding: :intake @15:25:06.50 Fix #1 of 2 (with RNAlater) for 2:30 @15:25:06.51 CSV.dial CSR @15:25:06.94 Pulling 2ml of CSR.RNAlater @15:25:06.95 CSR.select :RNAlater @15:25:07.07 CS.seek 2ml @15:25:13.08 delay 5 seconds @15:25:16.51 PSV.dial PSR @15:25:17.70 PSR.select :flush @15:25:17.83 PS.seek 0.5ml @15:25:18.10 CSR.select :air @15:25:18.22 CS.reconfigure :fast @15:25:18.28 CS.seek 10ml @15:25:23.83 PSR.select :air @15:25:23.95 PS.seek 2.5ml @15:25:37.90 Pumping wcr puck from tube 5 up to 35psi... @15:25:37.92 CBV.dial SSV @15:25:39.18 CBV.dial between CSV and waste2b @15:25:40.16 CSV.dial between air and CTV @15:25:40.76 CS.seek 6.94ml @15:25:45.20 PSV.dial PTV @15:25:45.97 PBV.dial PSV @15:25:46.54 PS.seek 0ml @15:25:48.65 CTV.dial CSV,avoiding: :air @15:25:48.68 CSV.dial CTV @15:25:50.04 CS.reconfigure :pressurize @15:25:50.10 CS.seek 0ml @15:26:07.70 delay 2:30 @15:26:12.86 PSV.dial PBV @15:26:13.33 PS.seek 2.5ml @15:26:39.64 PSV.dial :waste1 @15:26:40.40 PS.seek 0ml @15:27:06.79 PSV.dial PSR @15:27:07.26 PSR.select :air @15:27:07.29 PS.seek 2.5ml @15:27:33.82 PSV.dial PTV @15:27:34.56 PBV.dial PSV @15:27:34.75 PS.seek 0ml @15:28:01.26 PSV.dial PBV @15:28:01.74 PS.seek 2.5ml @15:28:28.10 PSV.dial :waste1 @15:28:28.85 PS.seek 0ml @15:28:37.72 CBV.dial :waste2b @15:28:38.06 CS.reconfigure :default @15:28:38.11 CS.seek 0ml @15:28:39.42 delay 1:00 @15:28:55.23 PSV.dial PSR @15:28:55.70 PSR.select :flush @15:28:56.26 PS.seek 1ml @15:29:07.26 PSR.select :air @15:29:07.38 PS.seek 2.5ml @15:29:23.48 PSV.dial PTV @15:29:24.21 PTV.dial :puck @15:29:24.29 PBV.dial :waste1 @15:29:24.84 PS.seek 0ml @15:29:39.45 CTV.dial :air,avoiding: :intake @15:29:40.79 Fix #2 of 2 (with RNAlater) for 2:30 @15:29:40.80 CSV.dial CSR @15:29:41.24 Pulling 2ml of CSR.RNAlater @15:29:41.25 CSR.select :RNAlater @15:29:41.37 CS.seek 2ml @15:29:47.27 delay 5 seconds @15:29:51.20 PSV.dial PSR @15:29:51.95 PSR.select :flush @15:29:52.07 PS.seek 0.5ml @15:29:52.28 CSR.select :air @15:29:52.41 CS.reconfigure :fast @15:29:52.46 CS.seek 10ml @15:29:58.04 PSR.select :air @15:29:58.16 PS.seek 2.5ml @15:30:12.11 Pumping wcr puck from tube 5 up to 35psi... @15:30:12.13 CBV.dial SSV @15:30:13.38 CBV.dial between CSV and waste2b @15:30:14.36 CSV.dial between air and CTV @15:30:14.96 CS.seek 6.925ml @15:30:19.41 PSV.dial PTV @15:30:20.18 PBV.dial PSV @15:30:20.75 PS.seek 0ml @15:30:22.74 CTV.dial CSV,avoiding: :air @15:30:22.77 CSV.dial CTV @15:30:24.15 CS.reconfigure :pressurize @15:30:24.20 CS.seek 0ml @15:30:39.13 delay 2:30 @15:30:47.16 PSV.dial PBV @15:30:47.64 PS.seek 2.5ml @15:31:14.01 PSV.dial :waste1 @15:31:14.77 PS.seek 0ml @15:31:41.13 PSV.dial PSR @15:31:42.05 PSR.select :air @15:31:42.07 PS.seek 2.5ml @15:32:08.41 PSV.dial PTV @15:32:09.15 PBV.dial PSV @15:32:09.34 PS.seek 0ml @15:32:35.66 PSV.dial PBV @15:32:36.14 PS.seek 2.5ml @15:33:02.44 PSV.dial :waste1 @15:33:03.18 PS.seek 0ml @15:33:09.15 CBV.dial :waste2b @15:33:09.49 CS.reconfigure :default @15:33:09.54 CS.seek 0ml @15:33:13.51 delay 1:00 @15:33:29.60 Garaging FlushPuck in Processing Clamp @15:33:29.67 Elbow.reconfigure :tight @15:33:29.72 Elbow.seek PC @15:33:29.85 Elbow.reconfigure :loose @15:33:29.95 Forearm.seek PC @15:33:30.90 Hand.close @15:33:31.53 PC.seek :open @15:33:36.28 Forearm.seek :retract @15:33:37.24 Elbow.reconfigure :tight @15:33:37.29 Elbow.seek :garage @15:33:38.66 Elbow.reconfigure :loose @15:33:38.76 Forearm.reconfigure :tight @15:33:38.81 Forearm.seek :garage @15:33:39.88 Hand.open @15:33:40.58 Forearm.reconfigure :loose @15:33:40.63 Forearm.seek :retract @15:34:13.54 CTV.dial :air,avoiding: :intake @15:34:14.90 Storing wcr puck in Collection Clamp to tube 4 @15:34:15.08 SC.reconfigure :tight @15:34:15.13 SC.seek 4 @15:34:15.19 SC.configure :limp @15:34:15.41 Elbow.reconfigure :tight @15:34:15.91 Elbow.seek 4 @15:34:17.77 Forearm.reconfigure :tight @15:34:17.83 Forearm.seek 4 + 450 counts @15:34:18.86 SE.reconfigure :pick @15:34:18.92 SE.seek :beamBreak @15:34:19.97 SE.configure :default @15:34:20.14 Elbow.reconfigure :limp @15:34:20.20 Forearm.reconfigure :loose @15:34:20.24 Forearm.seek 4 @15:34:20.49 Elbow.hold @15:34:20.56 Elbow.reconfigure :tight @15:34:20.66 SE.seek 31.09 Pucks below Top Plate @15:34:21.23 Forearm.force -20 @15:34:21.27 Forearm.seek 4 + 294 counts @15:34:21.67 Forearm.reconfigure :tight @15:34:21.72 Forearm.seek 4 + 450 counts @15:34:21.92 SE.seek 32.09 Pucks below Top Plate @15:34:22.48 Forearm.reconfigure :loose @15:34:22.53 Forearm.seek :retract @15:34:23.49 Elbow.seek CC @15:34:25.13 Elbow.reconfigure :loose @15:34:25.23 Forearm.seek CC @15:34:26.21 Hand.close @15:34:26.78 CC.seek :open @15:34:31.94 Forearm.seek :retract @15:34:33.00 Elbow.reconfigure :tight @15:34:33.05 Elbow.seek 4 @15:34:34.80 Elbow.reconfigure :limp @15:34:34.85 Forearm.seek 4 @15:34:35.88 Elbow.hold @15:34:35.95 Elbow.reconfigure :tight @15:34:36.00 Hand.open @15:34:36.77 Forearm.force -20 @15:34:36.82 Forearm.seek 4 + 320 counts @15:34:37.24 Forearm.reconfigure :tight @15:34:37.29 Forearm.seek 4 + 450 counts @15:34:37.54 SE.seek :down @15:34:38.05 SE.stop @15:34:38.67 Forearm.reconfigure :loose @15:34:38.72 Forearm.seek :retract @15:34:39.70 Starting finalPurge @15:34:39.71 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @15:34:39.90 Elbow.seek FlushPuck::Garage @15:34:41.78 Elbow.reconfigure :loose @15:34:41.88 Forearm.seek :garage @15:34:42.84 Hand.close @15:34:43.51 Forearm.seek :retract @15:34:44.49 Elbow.reconfigure :tight @15:34:44.54 Elbow.seek CC @15:34:47.44 Elbow.reconfigure :loose @15:34:47.54 Forearm.reconfigure :tight @15:34:47.58 Forearm.seek CC @15:34:48.81 CC.seek :closed @15:34:53.33 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.32 @15:34:53.40 Hand.open @15:34:54.08 Forearm.reconfigure :loose @15:34:54.13 Forearm.seek :retract @15:34:55.15 Flushing the Collection Valve::Manifold @15:34:55.27 CSV.dial CSR @15:34:55.72 Pulling 5ml of CSR.flush CSR.select :flush @15:34:55.84 CS.seek 5ml @15:35:09.45 delay 5 seconds @15:35:14.47 CSR.select :air @15:35:14.63 CS.reconfigure :fast @15:35:14.68 CS.seek 7.5ml @15:35:21.73 CSV.dial :waste @15:35:23.03 CS.reconfigure :default @15:35:23.08 CS.seek 0ml @15:35:43.11 Flushing lower manifold's waste1 port @15:35:43.23 CSV.dial :flush @15:35:43.67 CS.reconfigure :fast @15:35:43.72 CS.seek 5ml @15:35:56.57 delay 5 seconds @15:36:01.59 CSV.dial :air @15:36:02.15 CS.seek 7ml @15:36:07.76 CSV.dial CTV @15:36:07.79
CTV.dial CSV @15:36:09.20 CBV.dial :waste1 @15:36:09.97 CS.seek 0ml @15:36:26.45 Flushing lower manifold's waste2 port @15:36:26.57 CSV.dial :flush @15:36:27.28 CS.seek 5ml @15:36:40.17 delay 5 seconds @15:36:45.19 CSV.dial :air @15:36:45.70 CS.seek 6.999ml @15:36:51.21 CSV.dial CTV @15:36:51.23
CTV.dial CSV @15:36:51.87 CBV.dial :waste2 @15:36:52.92 CS.seek 0ml @15:37:09.39 Flushing lower manifold's waste2b port @15:37:09.51 CSV.dial :flush @15:37:10.24 CS.seek 5ml @15:37:23.07 delay 5 seconds @15:37:28.09 CSV.dial :air @15:37:28.62 CS.seek 6.999ml @15:37:34.29 CSV.dial CTV @15:37:34.32
CTV.dial CSV @15:37:34.76 CBV.dial :waste2b @15:37:35.81 CS.seek 0ml @15:37:52.42 Disinfecting external Intake with kill @15:37:52.54 CSV.dial CSR @15:37:53.09 Pulling 8ml of CSR.kill @15:37:53.10 CSR.select :kill @15:37:53.22 CS.seek 8ml @15:38:13.04 delay 5 seconds @15:38:18.06 CSR.select :air @15:38:18.18 CS.seek 10ml @15:38:23.71 CSV.dial CBV @15:38:23.74
CBV.dial CSV @15:38:24.79 CTV.dial :intake @15:38:25.54 Intake.select :open @15:38:25.65 CS.seek 2ml @15:38:44.48 Intake.select :close @15:38:44.58 CTV.dial :air @15:38:45.33 CS.reconfigure :default @15:38:45.38 CS.seek 0ml @15:38:51.51 CSV.dial CSR @15:38:52.52 Pulling 5ml of CSR.flush @15:38:52.53 CSR.select :flush @15:38:52.65 CS.seek 5ml @15:39:06.23 delay 5 seconds @15:39:11.25 CSR.select :air @15:39:11.36 CS.seek 10ml @15:39:24.91 CSV.dial CBV @15:39:25.94 CS.seek 0ml @15:39:52.29 Garaging FlushPuck in Collection Clamp @15:39:52.36 Elbow.reconfigure :tight @15:39:52.41 Elbow.seek CC @15:39:52.75 Elbow.reconfigure :loose @15:39:52.85 Forearm.seek CC @15:39:53.92 Hand.close @15:39:54.52 CC.seek :open @15:39:59.60 Forearm.seek :retract @15:40:00.60 Elbow.reconfigure :tight @15:40:00.65 Elbow.seek :garage @15:40:03.53 Elbow.reconfigure :loose @15:40:03.63 Forearm.reconfigure :tight @15:40:03.68 Forearm.seek :garage @15:40:04.68 Hand.open @15:40:05.33 Forearm.reconfigure :loose @15:40:05.38 Forearm.seek :retract @15:40:06.38 Completed [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] begun 13:11:31 @15:40:06.43 Asleep after completing DA_WCR_120723 assays @15:40:06.45 GoodNews.email "{1 => 33,\n 2 => 33,\n 3 => 31,\n 4 => 33,\n 5 => 0,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@15:30:52, 30% flow, 16.8C, 31% humidity, 15.1psia, 13.710V, 0.423A, 170.482Ah, 5.80W\nSampled 700.0ml",Subject: "Asleep after completing DA_WCR_120723 assays" @15:40:10.78 upsync + ESPwhich DA_WCR_120723b.rb + fn=DA_WCR_120723b.rb + IFS=: + set /home/esp/esp2/mission . + fullPath=/home/esp/esp2/mission/DA_WCR_120723b.rb + [ -f /home/esp/esp2/mission/DA_WCR_120723b.rb ] + realpath /home/esp/esp2/mission/DA_WCR_120723b.rb + return 0 + scriptPath=/home/esp/esp2local/mission/DA_WCR_120723b.rb + exec ruby /home/esp/esp2/lib/esp.rb /home/esp/esp2local/mission/DA_WCR_120723b.rb @21:24:31.44UTC07-Dec-23
ESP2 v3.42 I2C Gateway v3.20 for core bus @21:24:34.11 Configuration for short puck jake 1/11/21 by brent@mbari.org /home/esp/esp2/type/shallow/jake/configure.rb @21:24:37.80 Polling Can every 10:00 @21:24:37.90 Cleanly exited at 20:05:27UTC07-Dec-23 @21:24:37.95 Loading /home/esp/esp2local/mission/DA_WCR_120723b.rb mission @21:24:38.00 Preparing to sleep until + 00:00:05 @21:24:38.02 GoodNews.email "Starting DA_WCR_120723b w/pucks in tube 6\n{1 => 33,\n 2 => 33,\n 3 => 31,\n 4 => 33,\n 5 => 0,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@21:24:37, 30% flow, 17.1C, 32% humidity, 15.2psia, 13.749V, 0.272A, 171.929Ah, 3.74W",Subject: "DA_WCR_120723b begun" @21:24:38.07
Asleep til + 00:00:05 @21:24:38.11 Continuing at 21:24 @21:24:38.34 Gate.power :core,:OFF @21:24:38.36 Gate.power :raw,:OFF @21:24:38.37 Shutdown @21:24:41.52 GoodNews.email "Then, in tube 6: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_120723b.rb:14\n{1 => 33,\n 2 => 33,\n 3 => 31,\n 4 => 33,\n 5 => 0,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@21:24:38, 30% flow, 17.1C, 32% humidity, 15.2psia, 13.744V, 0.272A, 171.930Ah, 3.74W",Subject: "Asleep until 21:24" @21:24:42.90 upsync @21:24:43.00
Powering core bus @21:24:46.35 upsync @21:24:48.58
Gate.power :raw,:ON @21:24:49.52 CS.configure :default @21:24:49.57 PS.configure :default @21:24:49.61 SS.configure :default @21:24:49.66 CC.configure :default @21:24:49.71 PC.configure :default @21:24:49.78 Forearm.configure :loose @21:24:49.82 Elbow.configure :loose @21:24:49.89 SC.configure :loose @21:24:49.94 SE.configure :default @21:24:49.99 CH.configure :default @21:24:50.02 PH.configure :default @21:24:50.06 All dwarf controllers running firmware version 3.85 @21:24:50.17 Valve.reset @21:24:50.47 Intake.select :close @21:24:50.49 Exhaust.select :close @21:24:50.50 CTV.dial :air @21:24:50.59 PSV.dial :air @21:24:50.63 CSV.dial :waste1 @21:24:50.77
Forearm.home.seek :retract @21:24:51.12 SSV.dial :spit,avoiding: :exhaust @21:24:51.53 Hand.close @21:24:51.56
Elbow.reconfigure :loose @21:24:51.65 Elbow.jog 1500 @21:24:51.72 PS.home.seek 0ml @21:24:51.99 CS.reconfigure :fast @21:24:52.03 SS.home.seek 0ml @21:24:52.11 CS.home.seek 0ml @21:24:52.20
Elbow.reconfigure :loose @21:24:52.26 Elbow.home.seek :clear @21:24:55.54 SE.home.seek :down @21:24:56.16 SC.reconfigure :tight @21:24:56.23 SC.home.seek :initial @21:25:00.24 PS.stop @21:25:15.10
SC.configure :limp @21:25:15.15 Forearm.seek :retract @21:25:15.22 Elbow.seek :hammer @21:25:16.25 Forearm.reconfigure :tight @21:25:16.30 Forearm.seek retracted @21:25:16.90 PC.home.seek :open @21:25:16.98
CC.home.seek :open @21:25:20.38 Phase [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] @21:25:20.39 Notice.email "At 21:25:20 began in tube 6: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_120723b.rb:14\n{1 => 33,\n 2 => 33,\n 3 => 31,\n 4 => 33,\n 5 => 0,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@21:25:20, 30% flow, 17.1C, 32% humidity, 15.2psia, 13.681V, 0.296A, 171.934Ah, 4.06W",Subject: "Started DA_WCR_120723b phase initialPurge; da [1000, 1000] #da and wsr are run simultanelously" @21:25:20.40
Starting initialPurge @21:25:20.43 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @21:25:20.50 Hand.open @21:25:21.20 Elbow.reconfigure :tight @21:25:21.25 Elbow.seek FlushPuck::Garage @21:25:23.17 Elbow.reconfigure :loose @21:25:23.27 Forearm.reconfigure :loose @21:25:23.31 Forearm.seek :garage @21:25:24.29 Hand.close @21:25:24.86 upsync @21:25:25.06
Forearm.seek :retract @21:25:26.05 Elbow.reconfigure :tight @21:25:26.10 Elbow.seek CC @21:25:29.09 Elbow.reconfigure :loose @21:25:29.20 Forearm.reconfigure :tight @21:25:29.25 Forearm.seek CC @21:25:30.48 CC.seek :closed @21:25:35.02 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.40 @21:25:35.09 Hand.open @21:25:35.75 Forearm.reconfigure :loose @21:25:35.80 Forearm.seek :retract @21:25:36.83 Flushing the Collection Valve::Manifold @21:25:36.96 CSV.dial CSR @21:25:38.25 Pulling 5ml of CSR.flush @21:25:38.26 CSR.select :flush @21:25:38.37 CS.reconfigure :default @21:25:38.42 CS.seek 5ml @21:25:52.67 delay 5 seconds @21:25:57.69 CSR.select :air @21:25:57.85 CS.reconfigure :fast @21:25:57.90 CS.seek 7.499ml @21:26:04.76 CSV.dial :waste @21:26:06.06 CS.reconfigure :default @21:26:06.11 CS.seek 0ml @21:26:26.11 Purging external Intake @21:26:26.23 CSV.dial :flush @21:26:26.80 CS.reconfigure :fast @21:26:26.85 CS.seek 10ml @21:26:51.54 delay 5 seconds @21:26:56.57 CSV.dial CBV @21:26:56.59
CBV.dial CSV @21:26:57.35 CTV.dial :intake @21:26:58.15 Intake.select :open @21:26:58.26 CS.seek 0ml @21:27:21.85 Intake.select :close @21:27:21.96 CTV.dial :air @21:27:22.73 Flushing upper manifold @21:27:22.85 CSV.dial :flush @21:27:23.58 CS.seek 5ml @21:27:36.42 delay 5 seconds @21:27:41.44 CSV.dial :air @21:27:41.95 CS.seek 7.001ml @21:27:47.45 CSV.dial CBV @21:27:47.47
CBV.dial CSV @21:27:48.53 CTV.dial :air @21:27:48.54 CS.seek 0ml @21:28:05.23 delay 7 seconds @21:28:12.26 CS.seek 3ml @21:28:20.29 CSV.dial :waste @21:28:20.75 CS.seek 0ml @21:28:28.53 CSV.dial :flush @21:28:28.98 CS.seek 3ml @21:28:37.23 delay 5 seconds @21:28:42.25 CSV.dial :air @21:28:42.74 CS.seek 5ml @21:28:48.32 CSV.dial :air @21:28:48.41 CS.seek 0ml @21:29:00.51 delay 7 seconds @21:29:07.54 CS.seek 3ml @21:29:15.57 CSV.dial :waste @21:29:16.31 CS.seek 0ml @21:29:24.07 Garaging FlushPuck in Collection Clamp @21:29:24.14 Elbow.reconfigure :tight @21:29:24.19 Elbow.seek CC @21:29:24.51 Elbow.reconfigure :loose @21:29:24.61 Forearm.seek CC @21:29:25.65 Hand.close @21:29:26.31 CC.seek :open @21:29:31.34 Forearm.seek :retract @21:29:32.34 Elbow.reconfigure :tight @21:29:32.39 Elbow.seek :garage @21:29:35.27 Elbow.reconfigure :loose @21:29:35.37 Forearm.reconfigure :tight @21:29:35.41 Forearm.seek :garage @21:29:36.45 Hand.open @21:29:37.12 Forearm.reconfigure :loose @21:29:37.17 Forearm.seek :retract @21:29:38.16 da sampling at most 1000ml, wcr at most 1000ml @21:29:38.20 Loading sh1 puck from tube 6 into Collection Clamp @21:29:38.21 DAfans.email "Started da in tube 6 at 21:29:38UTC07-Dec-23\n{1 => 33,\n 2 => 33,\n 3 => 31,\n 4 => 33,\n 5 => 0,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@21:25:20, 30% flow, 17.1C, 32% humidity, 15.2psia, 13.681V, 0.296A, 171.934Ah, 4.06W",Subject: "da sampling at most 1000ml, wcr at most 1000ml" @21:29:38.49
SC.reconfigure :tight @21:29:38.55 SC.seek 6 @21:29:42.59 upsync @21:29:45.23
SC.configure :limp @21:29:45.46 Elbow.reconfigure :tight @21:29:45.51 Elbow.seek 6 @21:29:47.45 Forearm.reconfigure :tight @21:29:47.50 Forearm.seek 6 + 450 counts @21:29:48.70 SE.reconfigure :pick @21:29:48.76 SE.seek :beamBreak @21:29:49.73 SE.configure :default @21:29:49.90 Elbow.seek 6 @21:29:50.09 Forearm.seek 6 + 300 counts @21:29:50.29 Forearm.reconfigure :loose @21:29:50.34 Hand.close @21:29:50.95 SE.seek :down @21:29:51.16
Forearm.seek :retract @21:29:51.66 SE.stop @21:29:52.53
Elbow.seek CC @21:29:54.08 Elbow.reconfigure :loose @21:29:54.18 Forearm.reconfigure :tight @21:29:54.23 Forearm.seek CC @21:29:55.54 CC.seek :closed @21:30:00.07 Collection Clamp onSpring/offSpring for 13/27 samples, on/off torque 2.39 @21:30:00.08 Hand.open @21:30:00.74 Forearm.reconfigure :loose @21:30:00.79 Forearm.seek :retract @21:30:01.76 collectSample 1000ml w/bubble point of 24psi @21:30:01.78 Priming 7ml @21:30:01.79 Intake.select :close @21:30:01.80 Exhaust.select :close @21:30:01.81 SS.hold @21:30:02.02 Calibrating pressure and temperature sensors @21:30:02.04 CTV.dial :air @21:30:02.06 SSV.dial :spit,avoiding: :exhaust @21:30:02.14 SS.reconfigure :fast @21:30:02.18 SS.seek 5ml @21:30:05.01 SSV.dial CBV @21:30:05.05
CBV.dial SSV @21:30:06.36 delay 3.8 seconds @21:30:10.30 Sensor limits: Intake max 101.0psia; Outlet max 94.1psia @21:30:10.31 SSV.dial :spit @21:30:11.64 SS.seek 0ml @21:30:14.47 SSV.dial CBV @21:30:15.81 CTV.dial :intake @21:30:16.59 SSV.dial CBV @21:30:16.61
CBV.dial SSV @21:30:16.82 Allowing 40s for 7.0ml of remaining 7.0ml @21:30:16.83 Intake.select :open @21:30:18.99 Intake @14.4psia, vacuum from 8.9 to 11.9psi @21:30:19.01 SS.reconfigure :inhaling 2.20ml/s @21:30:19.05 SS.seek 7ml @21:30:25.81 Stroke completed -- waiting for pressure to equalize ... @21:30:25.82 SS.reconfigure :exhale @21:30:25.87 SS.hold @21:30:35.81 Intake.select :close @21:30:35.97 SSV.dial :exhaust @21:30:36.74 Exhaust.select :open @21:30:36.85 SS.seek 0ml @21:30:40.92 Exhaust.select :close @21:30:41.03 Sampling 1000.0ml @21:30:41.05 Intake.select :close @21:30:41.06 Exhaust.select :close @21:30:41.07 SS.reconfigure :default @21:30:41.11 SS.hold @21:30:41.32 CTV.dial :intake @21:30:41.40 SSV.dial CBV @21:30:41.42
CBV.dial SSV @21:30:42.21 Allowing 130s for 25.0ml of remaining 1000.0ml @21:30:42.22 Intake.select :open @21:30:44.38 Intake @15.3psia, vacuum from 9.8 to 12.8psi @21:30:44.39 SS.reconfigure :inhaling 2.20ml/s @21:30:44.44 SS.seek 25ml @21:30:52.67 Vacuum too high -- waiting up to 40 seconds @21:30:52.68 SS.reconfigure :exhale @21:30:52.73 SS.hold @21:30:58.44 Intake @17.5psia, vacuum from 10.0 to 13.0psi @21:30:58.45 SS.reconfigure :inhaling 1.76ml/s @21:30:58.50 SS.seek 25ml @21:31:01.39 Vacuum too high -- waiting up to 40 seconds @21:31:01.40 SS.reconfigure :exhale @21:31:01.45 SS.hold @21:31:03.30 Intake @16.3psia, vacuum from 10.0 to 13.0psi @21:31:03.32 SS.reconfigure :inhaling 1.41ml/s @21:31:03.37 SS.seek 25ml @21:31:08.95 Vacuum too high -- waiting up to 40 seconds @21:31:08.96 SS.reconfigure :exhale @21:31:09.01 SS.hold @21:31:12.12 Intake @17.0psia, vacuum from 10.0 to 13.0psi @21:31:12.13 SS.reconfigure :inhaling 1.13ml/s @21:31:12.18 SS.seek 25ml @21:31:15.81 Stroke completed -- waiting for pressure to equalize ... @21:31:15.82 SS.reconfigure :exhale @21:31:15.87 SS.hold @21:31:44.43 Intake.select :close @21:31:44.58 SSV.dial :exhaust @21:31:45.33 Exhaust.select :open @21:31:45.44 SS.seek 0ml @21:31:54.27 Exhaust.select :close @21:31:54.37 SSV.dial CBV @21:31:54.39
CBV.dial SSV @21:31:55.17 Allowing 130s for 25.0ml of remaining 975.0ml @21:31:55.18 Intake.select :open @21:31:57.35 Intake @15.9psia, vacuum from 10.0 to 13.0psi @21:31:57.37 SS.reconfigure :inhaling 1.13ml/s @21:31:57.42 SS.seek 25ml @21:32:22.19 Stroke completed -- waiting for pressure to equalize ... @21:32:22.20 SS.reconfigure :exhale @21:32:22.24 SS.hold @21:32:59.51 Intake.select :close @21:32:59.66 SSV.dial :exhaust @21:33:00.41 Exhaust.select :open @21:33:00.52 SS.seek 0ml @21:33:09.37 Exhaust.select :close @21:33:09.48 SSV.dial CBV @21:33:09.51
CBV.dial SSV @21:33:10.28 Allowing 130s for 25.0ml of remaining 950.0ml @21:33:10.29 Intake.select :open @21:33:12.46 Intake @15.9psia, vacuum from 10.0 to 13.0psi @21:33:12.47 SS.reconfigure :inhaling 1.13ml/s @21:33:12.52 SS.seek 25ml @21:33:37.56 Stroke completed -- waiting for pressure to equalize ... @21:33:37.57 SS.reconfigure :exhale @21:33:37.62 SS.hold @21:34:14.33 Intake.select :close @21:34:14.48 SSV.dial :exhaust @21:34:15.25 Exhaust.select :open @21:34:15.36 SS.seek 0ml @21:34:23.98 Exhaust.select :close @21:34:24.09 SSV.dial CBV @21:34:24.12
CBV.dial SSV @21:34:24.89 Allowing 130s for 25.0ml of remaining 925.0ml @21:34:24.90 Intake.select :open @21:34:27.06 Intake @16.0psia, vacuum from 10.0 to 13.0psi @21:34:27.08 SS.reconfigure :inhaling 1.13ml/s @21:34:27.13 SS.seek 25ml @21:34:52.03 Stroke completed -- waiting for pressure to equalize ... @21:34:52.04 SS.reconfigure :exhale @21:34:52.08 SS.hold @21:35:27.22 Intake.select :close @21:35:27.37 SSV.dial :exhaust @21:35:28.14 Exhaust.select :open @21:35:28.25 SS.seek 0ml @21:35:37.25 Exhaust.select :close @21:35:37.35 SSV.dial CBV @21:35:37.37
CBV.dial SSV @21:35:38.15 Allowing 130s for 25.0ml of remaining 900.0ml @21:35:38.17 Intake.select :open @21:35:40.33 Intake @16.0psia, vacuum from 10.0 to 13.0psi @21:35:40.35 SS.reconfigure :inhaling 1.13ml/s @21:35:40.40 SS.seek 25ml @21:36:05.13 Stroke completed -- waiting for pressure to equalize ... @21:36:05.21 SS.reconfigure :exhale @21:36:05.26 SS.hold @21:36:35.74 Intake.select :close @21:36:35.99 SSV.dial :exhaust @21:36:36.76 Exhaust.select :open @21:36:36.88 SS.seek 0ml @21:36:45.64 Exhaust.select :close @21:36:45.74 SSV.dial CBV @21:36:45.77
CBV.dial SSV @21:36:46.56 Allowing 130s for 25.0ml of remaining 875.0ml @21:36:46.57 Intake.select :open @21:36:49.09 Intake @16.1psia, vacuum from 10.0 to 13.0psi @21:36:49.18 SS.reconfigure :inhaling 1.13ml/s @21:36:49.23 SS.seek 25ml @21:37:14.04 Stroke completed -- waiting for pressure to equalize ... @21:37:14.05 SS.reconfigure :exhale @21:37:14.10 SS.hold @21:37:44.76 Intake.select :close @21:37:44.91 SSV.dial :exhaust @21:37:45.67 Exhaust.select :open @21:37:45.78 SS.seek 0ml @21:37:54.62 Exhaust.select :close @21:37:54.73 SSV.dial CBV @21:37:54.75
CBV.dial SSV @21:37:55.54 Allowing 130s for 25.0ml of remaining 850.0ml @21:37:55.55 Intake.select :open @21:37:57.72 Intake @16.0psia, vacuum from 10.0 to 13.0psi @21:37:57.74 SS.reconfigure :inhaling 1.13ml/s @21:37:57.79 SS.seek 25ml @21:38:22.35 Stroke completed -- waiting for pressure to equalize ... @21:38:22.36 SS.reconfigure :exhale @21:38:22.41 SS.hold @21:38:51.03 Intake.select :close @21:38:51.19 SSV.dial :exhaust @21:38:51.95 Exhaust.select :open @21:38:52.05 SS.seek 0ml @21:39:00.84 Exhaust.select :close @21:39:00.95 SSV.dial CBV @21:39:01.41
CBV.dial SSV @21:39:02.16 Allowing 130s for 25.0ml of remaining 825.0ml @21:39:02.18 Intake.select :open @21:39:04.34 Intake @15.9psia, vacuum from 10.0 to 13.0psi @21:39:04.35 SS.reconfigure :inhaling 1.13ml/s @21:39:04.40 SS.seek 25ml @21:39:28.40 Vacuum too high -- waiting up to 40 seconds @21:39:28.42 SS.reconfigure :exhale @21:39:28.47 SS.hold @21:39:38.35 Intake @17.8psia, vacuum from 10.0 to 13.0psi @21:39:38.37 SS.reconfigure :inhaling 0.91ml/s @21:39:38.42 SS.seek 25ml @21:39:40.00 Stroke completed -- waiting for pressure to equalize ... @21:39:40.01 SS.reconfigure :exhale @21:39:40.06 SS.hold @21:39:54.44 Intake.select :close @21:39:54.60 SSV.dial :exhaust @21:39:55.37 Exhaust.select :open @21:39:55.48 SS.seek 0ml @21:40:04.19 Exhaust.select :close @21:40:04.30 SSV.dial CBV @21:40:04.33
CBV.dial SSV @21:40:05.10 Allowing 130s for 25.0ml of remaining 800.0ml @21:40:05.11 Intake.select :open @21:40:07.28 Intake @16.1psia, vacuum from 10.0 to 13.0psi @21:40:07.29 SS.reconfigure :inhaling 0.91ml/s @21:40:07.34 SS.seek 25ml @21:40:37.16 Stroke completed -- waiting for pressure to equalize ... @21:40:37.17 SS.reconfigure :exhale @21:40:37.22 SS.hold @21:40:57.64 Intake.select :close @21:40:57.80 SSV.dial :exhaust @21:40:58.56 Exhaust.select :open @21:40:58.68 SS.seek 0ml @21:41:07.44 Exhaust.select :close @21:41:07.55 SSV.dial CBV @21:41:07.57
CBV.dial SSV @21:41:08.35 Allowing 130s for 25.0ml of remaining 775.0ml @21:41:08.36 Intake.select :open @21:41:10.53 Intake @16.1psia, vacuum from 10.0 to 13.0psi @21:41:10.54 SS.reconfigure :inhaling 0.91ml/s @21:41:10.60 SS.seek 25ml @21:41:40.49 Stroke completed -- waiting for pressure to equalize ... @21:41:40.50 SS.reconfigure :exhale @21:41:40.54 SS.hold @21:42:00.92 Intake.select :close @21:42:01.09 SSV.dial :exhaust @21:42:01.86 Exhaust.select :open @21:42:01.97 SS.seek 0ml @21:42:10.72 Exhaust.select :close @21:42:10.84 SSV.dial CBV @21:42:10.86
CBV.dial SSV @21:42:11.65 Allowing 130s for 25.0ml of remaining 750.0ml @21:42:11.66 Intake.select :open @21:42:13.82 Intake @16.2psia, vacuum from 10.0 to 13.0psi @21:42:13.83 SS.reconfigure :inhaling 0.91ml/s @21:42:13.89 SS.seek 25ml @21:42:44.10 Stroke completed -- waiting for pressure to equalize ... @21:42:44.11 SS.reconfigure :exhale @21:42:44.15 SS.hold @21:43:04.55 Intake.select :close @21:43:04.71 SSV.dial :exhaust @21:43:05.48 Exhaust.select :open @21:43:06.02 SS.seek 0ml @21:43:14.92 Exhaust.select :close @21:43:15.04 SSV.dial CBV @21:43:15.08
CBV.dial SSV @21:43:15.82 Allowing 130s for 25.0ml of remaining 725.0ml @21:43:15.83 Intake.select :open @21:43:17.99 Intake @16.2psia, vacuum from 10.0 to 13.0psi @21:43:18.00 SS.reconfigure :inhaling 0.91ml/s @21:43:18.06 SS.seek 25ml @21:43:47.74 Stroke completed -- waiting for pressure to equalize ... @21:43:47.75 SS.reconfigure :exhale @21:43:47.79 SS.hold @21:44:10.21 Intake.select :close @21:44:10.37 SSV.dial :exhaust @21:44:11.13 Exhaust.select :open @21:44:11.24 SS.seek 0ml @21:44:20.00 Exhaust.select :close @21:44:20.11 SSV.dial CBV @21:44:20.13
CBV.dial SSV @21:44:20.91 Allowing 130s for 25.0ml of remaining 700.0ml @21:44:20.92 Intake.select :open @21:44:23.51 Intake @16.1psia, vacuum from 10.0 to 13.0psi @21:44:23.52 SS.reconfigure :inhaling 0.91ml/s @21:44:23.58 SS.seek 25ml @21:44:53.47 Stroke completed -- waiting for pressure to equalize ... @21:44:53.48 SS.reconfigure :exhale @21:44:53.53 SS.hold @21:45:13.91 Intake.select :close @21:45:14.06 SSV.dial :exhaust @21:45:14.84 Exhaust.select :open @21:45:14.95 SS.seek 0ml @21:45:23.97 Exhaust.select :close @21:45:24.08 SSV.dial CBV @21:45:24.11
CBV.dial SSV @21:45:24.89 Allowing 130s for 25.0ml of remaining 675.0ml @21:45:24.90 Intake.select :open @21:45:27.07 Intake @16.2psia, vacuum from 10.0 to 13.0psi @21:45:27.09 SS.reconfigure :inhaling 0.91ml/s @21:45:27.14 SS.seek 25ml @21:45:56.69 Stroke completed -- waiting for pressure to equalize ... @21:45:56.70 SS.reconfigure :exhale @21:45:56.74 SS.hold @21:46:17.39 Intake.select :close @21:46:17.55 SSV.dial :exhaust @21:46:18.32 Exhaust.select :open @21:46:18.43 SS.seek 0ml @21:46:27.19 Exhaust.select :close @21:46:27.29 SSV.dial CBV @21:46:27.31
CBV.dial SSV @21:46:28.11 Allowing 130s for 25.0ml of remaining 650.0ml @21:46:28.12 Intake.select :open @21:46:30.29 Intake @16.3psia, vacuum from 10.0 to 13.0psi @21:46:30.30 SS.reconfigure :inhaling 0.91ml/s @21:46:30.35 SS.seek 25ml @21:47:00.25 Stroke completed -- waiting for pressure to equalize ... @21:47:00.26 SS.reconfigure :exhale @21:47:00.30 SS.hold @21:47:22.83 Intake.select :close @21:47:22.98 SSV.dial :exhaust @21:47:23.73 Exhaust.select :open @21:47:23.85 SS.seek 0ml @21:47:32.54 Exhaust.select :close @21:47:32.65 SSV.dial CBV @21:47:32.68
CBV.dial SSV @21:47:33.44 Allowing 130s for 25.0ml of remaining 625.0ml @21:47:33.45 Intake.select :open @21:47:35.61 Intake @16.2psia, vacuum from 10.0 to 13.0psi @21:47:35.62 SS.reconfigure :inhaling 0.91ml/s @21:47:35.67 SS.seek 25ml @21:48:02.21 Vacuum too high -- waiting up to 40 seconds @21:48:02.22 SS.reconfigure :exhale @21:48:02.27 SS.hold @21:48:10.98 Intake @16.9psia, vacuum from 10.0 to 13.0psi @21:48:10.99 SS.reconfigure :inhaling 0.73ml/s @21:48:11.04 SS.seek 25ml @21:48:15.39 Stroke completed -- waiting for pressure to equalize ... @21:48:15.40 SS.reconfigure :exhale @21:48:15.44 SS.hold @21:48:31.75 Intake.select :close @21:48:31.90 SSV.dial :exhaust @21:48:32.65 Exhaust.select :open @21:48:32.77 SS.seek 0ml @21:48:42.23 Exhaust.select :close @21:48:42.34 SSV.dial CBV @21:48:42.36
CBV.dial SSV @21:48:43.15 Allowing 130s for 25.0ml of remaining 600.0ml @21:48:43.16 Intake.select :open @21:48:45.33 Intake @16.1psia, vacuum from 10.0 to 13.0psi @21:48:45.35 SS.reconfigure :inhaling 0.73ml/s @21:48:45.40 SS.seek 25ml @21:49:21.39 Stroke completed -- waiting for pressure to equalize ... @21:49:21.40 SS.reconfigure :exhale @21:49:21.44 SS.hold @21:49:41.89 Intake.select :close @21:49:42.04 SSV.dial :exhaust @21:49:42.82 Exhaust.select :open @21:49:42.93 SS.seek 0ml @21:49:51.87 Exhaust.select :close @21:49:51.98 SSV.dial CBV @21:49:52.00
CBV.dial SSV @21:49:52.78 Allowing 130s for 25.0ml of remaining 575.0ml @21:49:52.79 Intake.select :open @21:49:55.30 Intake @16.3psia, vacuum from 10.0 to 13.0psi @21:49:55.39 SS.reconfigure :inhaling 0.73ml/s @21:49:55.44 SS.seek 25ml @21:50:31.21 Stroke completed -- waiting for pressure to equalize ... @21:50:31.22 SS.reconfigure :exhale @21:50:31.27 SS.hold @21:50:53.73 Intake.select :close @21:50:53.88 SSV.dial :exhaust @21:50:54.63 Exhaust.select :open @21:50:54.75 SS.seek 0ml @21:51:03.76 Exhaust.select :close @21:51:03.87 SSV.dial CBV @21:51:03.89
CBV.dial SSV @21:51:04.67 Allowing 130s for 25.0ml of remaining 550.0ml @21:51:04.68 Intake.select :open @21:51:06.84 Intake @16.3psia, vacuum from 10.0 to 13.0psi @21:51:06.85 SS.reconfigure :inhaling 0.73ml/s @21:51:06.90 SS.seek 25ml @21:51:43.02 Stroke completed -- waiting for pressure to equalize ... @21:51:43.03 SS.reconfigure :exhale @21:51:43.08 SS.hold @21:52:05.51 Intake.select :close @21:52:05.66 SSV.dial :exhaust @21:52:06.42 Exhaust.select :open @21:52:06.53 SS.seek 0ml @21:52:15.23 Exhaust.select :close @21:52:15.33 SSV.dial CBV @21:52:15.35
CBV.dial SSV @21:52:16.12 Allowing 130s for 25.0ml of remaining 525.0ml @21:52:16.13 Intake.select :open @21:52:18.29 Intake @16.3psia, vacuum from 10.0 to 13.0psi @21:52:18.30 SS.reconfigure :inhaling 0.73ml/s @21:52:18.35 SS.seek 25ml @21:52:51.67 Vacuum too high -- waiting up to 40 seconds @21:52:51.68 SS.reconfigure :exhale @21:52:51.73 SS.hold @21:53:00.65 Intake @16.7psia, vacuum from 10.0 to 13.0psi @21:53:00.67 SS.reconfigure :inhaling 0.59ml/s @21:53:00.72 SS.seek 25ml @21:53:04.36 Stroke completed -- waiting for pressure to equalize ... @21:53:04.37 SS.reconfigure :exhale @21:53:04.42 SS.hold @21:53:18.78 Intake.select :close @21:53:18.93 SSV.dial :exhaust @21:53:19.69 Exhaust.select :open @21:53:19.81 SS.seek 0ml @21:53:28.80 Exhaust.select :close @21:53:28.91 SSV.dial CBV @21:53:28.93
CBV.dial SSV @21:53:29.73 Allowing 130s for 25.0ml of remaining 500.0ml @21:53:29.74 Intake.select :open @21:53:31.90 Intake @16.3psia, vacuum from 10.0 to 13.0psi @21:53:31.91 SS.reconfigure :inhaling 0.59ml/s @21:53:31.96 SS.seek 25ml @21:54:16.11 Stroke completed -- waiting for pressure to equalize ... @21:54:16.12 SS.reconfigure :exhale @21:54:16.17 SS.hold @21:54:36.56 Intake.select :close @21:54:36.71 SSV.dial :exhaust @21:54:37.49 Exhaust.select :open @21:54:37.60 SS.seek 0ml @21:54:46.55 Exhaust.select :close @21:54:46.66 SSV.dial CBV @21:54:46.68
CBV.dial SSV @21:54:47.50 Allowing 130s for 25.0ml of remaining 475.0ml @21:54:47.51 Intake.select :open @21:54:49.68 Intake @16.3psia, vacuum from 10.0 to 13.0psi @21:54:49.70 SS.reconfigure :inhaling 0.59ml/s @21:54:49.74 SS.seek 25ml @21:55:34.19 Stroke completed -- waiting for pressure to equalize ... @21:55:34.20 SS.reconfigure :exhale @21:55:34.25 SS.hold @21:55:57.03 Intake.select :close @21:55:57.18 SSV.dial :exhaust @21:55:57.94 Exhaust.select :open @21:55:58.06 SS.seek 0ml @21:56:07.05 Exhaust.select :close @21:56:07.16 SSV.dial CBV @21:56:07.18
CBV.dial SSV @21:56:07.97 Allowing 130s for 25.0ml of remaining 450.0ml @21:56:07.98 Intake.select :open @21:56:10.15 Intake @16.1psia, vacuum from 10.0 to 13.0psi @21:56:10.16 SS.reconfigure :inhaling 0.59ml/s @21:56:10.22 SS.seek 25ml @21:56:54.66 Stroke completed -- waiting for pressure to equalize ... @21:56:54.67 SS.reconfigure :exhale @21:56:54.71 SS.hold @21:57:17.05 Intake.select :close @21:57:17.21 SSV.dial :exhaust @21:57:18.41 Exhaust.select :open @21:57:18.52 SS.seek 0ml @21:57:27.35 Exhaust.select :close @21:57:27.45 SSV.dial CBV @21:57:27.47
CBV.dial SSV @21:57:28.28 Allowing 130s for 25.0ml of remaining 425.0ml @21:57:28.30 Intake.select :open @21:57:30.46 Intake @16.4psia, vacuum from 10.0 to 13.0psi @21:57:30.47 SS.reconfigure :inhaling 0.59ml/s @21:57:30.52 SS.seek 25ml @21:58:15.14 Stroke completed -- waiting for pressure to equalize ... @21:58:15.15 SS.reconfigure :exhale @21:58:15.20 SS.hold @21:58:39.66 Intake.select :close @21:58:39.81 SSV.dial :exhaust @21:58:40.99 Exhaust.select :open @21:58:41.11 SS.seek 0ml @21:58:50.11 Exhaust.select :close @21:58:50.22 SSV.dial CBV @21:58:50.24
CBV.dial SSV @21:58:51.03 Allowing 130s for 25.0ml of remaining 400.0ml @21:58:51.04 Intake.select :open @21:58:53.21 Intake @16.4psia, vacuum from 10.0 to 13.0psi @21:58:53.23 SS.reconfigure :inhaling 0.59ml/s @21:58:53.27 SS.seek 25ml @21:59:31.45 Vacuum too high -- waiting up to 40 seconds @21:59:31.46 SS.reconfigure :exhale @21:59:31.51 SS.hold @21:59:40.15 Intake @17.2psia, vacuum from 10.0 to 13.0psi @21:59:40.16 SS.reconfigure :inhaling 0.47ml/s @21:59:40.21 SS.seek 25ml @21:59:47.78 Stroke completed -- waiting for pressure to equalize ... @21:59:47.79 SS.reconfigure :exhale @21:59:47.84 SS.hold @22:00:08.13 Intake.select :close @22:00:08.28 SSV.dial :exhaust @22:00:09.05 Exhaust.select :open @22:00:09.17 SS.seek 0ml @22:00:17.95 Exhaust.select :close @22:00:18.06 SSV.dial CBV @22:00:18.08
CBV.dial SSV @22:00:18.92 Allowing 130s for 25.0ml of remaining 375.0ml @22:00:18.93 Intake.select :open @22:00:21.10 Intake @16.3psia, vacuum from 10.0 to 13.0psi @22:00:21.12 SS.reconfigure :inhaling 0.47ml/s @22:00:21.17 SS.seek 25ml @22:01:16.43 Stroke completed -- waiting for pressure to equalize ... @22:01:16.44 SS.reconfigure :exhale @22:01:16.49 SS.hold @22:01:41.00 Intake.select :close @22:01:41.15 SSV.dial :exhaust @22:01:41.90 Exhaust.select :open @22:01:42.02 SS.seek 0ml @22:01:50.79 Exhaust.select :close @22:01:50.90 SSV.dial CBV @22:01:50.93
CBV.dial SSV @22:01:51.70 Allowing 130s for 25.0ml of remaining 350.0ml @22:01:51.71 Intake.select :open @22:01:53.88 Intake @16.4psia, vacuum from 10.0 to 13.0psi @22:01:53.89 SS.reconfigure :inhaling 0.47ml/s @22:01:53.94 SS.seek 25ml @22:02:49.23 Stroke completed -- waiting for pressure to equalize ... @22:02:49.24 SS.reconfigure :exhale @22:02:49.29 SS.hold @22:03:16.15 Intake.select :close @22:03:16.30 SSV.dial :exhaust @22:03:17.07 Exhaust.select :open @22:03:17.18 SS.seek 0ml @22:03:25.98 Exhaust.select :close @22:03:26.09 SSV.dial CBV @22:03:26.11
CBV.dial SSV @22:03:26.87 Allowing 130s for 25.0ml of remaining 325.0ml @22:03:26.88 Intake.select :open @22:03:29.05 Intake @16.4psia, vacuum from 10.0 to 13.0psi @22:03:29.06 SS.reconfigure :inhaling 0.47ml/s @22:03:29.12 SS.seek 25ml @22:04:24.62 Stroke completed -- waiting for pressure to equalize ... @22:04:24.63 SS.reconfigure :exhale @22:04:24.67 SS.hold @22:04:51.21 Intake.select :close @22:04:51.36 SSV.dial :exhaust @22:04:52.13 Exhaust.select :open @22:04:52.24 SS.seek 0ml @22:05:01.06 Exhaust.select :close @22:05:01.17 SSV.dial CBV @22:05:01.19
CBV.dial SSV @22:05:01.96 Allowing 130s for 25.0ml of remaining 300.0ml @22:05:01.97 Intake.select :open @22:05:04.14 Intake @16.5psia, vacuum from 10.0 to 13.0psi @22:05:04.15 SS.reconfigure :inhaling 0.47ml/s @22:05:04.20 SS.seek 25ml @22:05:56.87 Vacuum too high -- waiting up to 40 seconds @22:05:56.89 SS.reconfigure :exhale @22:05:56.94 SS.hold @22:06:08.17 Intake @18.2psia, vacuum from 10.0 to 13.0psi @22:06:08.19 SS.reconfigure :inhaling 0.38ml/s @22:06:08.24 SS.seek 25ml @22:06:11.80 Stroke completed -- waiting for pressure to equalize ... @22:06:11.81 SS.reconfigure :exhale @22:06:11.86 SS.hold @22:06:33.06 Intake.select :close @22:06:33.29 SSV.dial :exhaust @22:06:34.05 Exhaust.select :open @22:06:34.22 SS.seek 0ml @22:06:43.21 Exhaust.select :close @22:06:43.32 SSV.dial CBV @22:06:43.34
CBV.dial SSV @22:06:44.13 Allowing 130s for 25.0ml of remaining 275.0ml @22:06:44.14 Intake.select :open @22:06:46.31 Intake @16.3psia, vacuum from 10.0 to 13.0psi @22:06:46.33 SS.reconfigure :inhaling 0.38ml/s @22:06:46.37 SS.seek 25ml @22:07:53.91 Stroke completed -- waiting for pressure to equalize ... @22:07:53.92 SS.reconfigure :exhale @22:07:53.97 SS.hold @22:08:23.00 Intake.select :close @22:08:23.16 SSV.dial :exhaust @22:08:23.91 Exhaust.select :open @22:08:24.03 SS.seek 0ml @22:08:32.83 Exhaust.select :close @22:08:32.94 SSV.dial CBV @22:08:32.96
CBV.dial SSV @22:08:33.77 Allowing 130s for 25.0ml of remaining 250.0ml @22:08:33.78 Intake.select :open @22:08:35.95 Intake @16.5psia, vacuum from 10.0 to 13.0psi @22:08:35.96 SS.reconfigure :inhaling 0.38ml/s @22:08:36.06 SS.seek 25ml @22:09:44.58 Stroke completed -- waiting for pressure to equalize ... @22:09:44.59 SS.reconfigure :exhale @22:09:44.64 SS.hold @22:10:13.20 Intake.select :close @22:10:13.36 SSV.dial :exhaust @22:10:14.11 Exhaust.select :open @22:10:14.23 SS.seek 0ml @22:10:23.27 Exhaust.select :close @22:10:23.38 SSV.dial CBV @22:10:23.40
CBV.dial SSV @22:10:24.19 Allowing 130s for 25.0ml of remaining 225.0ml @22:10:24.20 Intake.select :open @22:10:26.37 Intake @16.4psia, vacuum from 10.0 to 13.0psi @22:10:26.38 SS.reconfigure :inhaling 0.38ml/s @22:10:26.43 SS.seek 25ml @22:11:34.69 Stroke completed -- waiting for pressure to equalize ... @22:11:34.70 SS.reconfigure :exhale @22:11:34.74 SS.hold @22:12:05.41 Intake.select :close @22:12:05.57 SSV.dial :exhaust @22:12:06.33 Exhaust.select :open @22:12:06.45 SS.seek 0ml @22:12:15.48 Exhaust.select :close @22:12:15.59 SSV.dial CBV @22:12:15.63
CBV.dial SSV @22:12:16.36 Allowing 130s for 25.0ml of remaining 200.0ml @22:12:16.37 Intake.select :open @22:12:18.54 Intake @16.5psia, vacuum from 10.0 to 13.0psi @22:12:18.55 SS.reconfigure :inhaling 0.38ml/s @22:12:18.60 SS.seek 25ml @22:13:26.47 Stroke completed -- waiting for pressure to equalize ... @22:13:26.48 SS.reconfigure :exhale @22:13:26.52 SS.hold @22:14:01.23 Intake.select :close @22:14:01.38 SSV.dial :exhaust @22:14:02.14 Exhaust.select :open @22:14:02.25 SS.seek 0ml @22:14:11.12 Exhaust.select :close @22:14:11.24 SSV.dial CBV @22:14:11.26
CBV.dial SSV @22:14:12.05 Allowing 130s for 25.0ml of remaining 175.0ml @22:14:12.06 Intake.select :open @22:14:14.22 Intake @16.7psia, vacuum from 10.0 to 13.0psi @22:14:14.24 SS.reconfigure :inhaling 0.38ml/s @22:14:14.28 SS.seek 25ml @22:15:22.92 Stroke completed -- waiting for pressure to equalize ... @22:15:22.93 SS.reconfigure :exhale @22:15:22.97 SS.hold @22:15:57.59 Intake.select :close @22:15:57.74 SSV.dial :exhaust @22:15:58.50 Exhaust.select :open @22:15:58.61 SS.seek 0ml @22:16:07.42 Exhaust.select :close @22:16:07.53 SSV.dial CBV @22:16:07.55
CBV.dial SSV @22:16:08.32 Allowing 130s for 25.0ml of remaining 150.0ml @22:16:08.34 Intake.select :open @22:16:10.50 Intake @16.6psia, vacuum from 10.0 to 13.0psi @22:16:10.51 SS.reconfigure :inhaling 0.38ml/s @22:16:10.56 SS.seek 25ml @22:17:19.59 Stroke completed -- waiting for pressure to equalize ... @22:17:19.60 SS.reconfigure :exhale @22:17:19.64 SS.hold @22:17:58.54 Intake.select :close @22:17:58.69 SSV.dial :exhaust @22:17:59.45 Exhaust.select :open @22:17:59.56 SS.seek 0ml @22:18:08.60 Exhaust.select :close @22:18:08.71 SSV.dial CBV @22:18:08.74
CBV.dial SSV @22:18:09.51 Allowing 130s for 25.0ml of remaining 125.0ml @22:18:09.52 Intake.select :open @22:18:11.69 Intake @16.6psia, vacuum from 10.0 to 13.0psi @22:18:11.70 SS.reconfigure :inhaling 0.38ml/s @22:18:11.75 SS.seek 25ml @22:19:19.93 Stroke completed -- waiting for pressure to equalize ... @22:19:19.94 SS.reconfigure :exhale @22:19:19.99 SS.hold @22:19:58.94 Intake.select :close @22:19:59.09 SSV.dial :exhaust @22:19:59.86 Exhaust.select :open @22:19:59.97 SS.seek 0ml @22:20:08.79 Exhaust.select :close @22:20:08.90 SSV.dial CBV @22:20:08.92
CBV.dial SSV @22:20:09.70 Allowing 130s for 25.0ml of remaining 100.0ml @22:20:09.71 Intake.select :open @22:20:11.87 Intake @16.6psia, vacuum from 10.0 to 13.0psi @22:20:11.88 SS.reconfigure :inhaling 0.38ml/s @22:20:11.93 SS.seek 25ml @22:21:20.38 Stroke completed -- waiting for pressure to equalize ... @22:21:20.39 SS.reconfigure :exhale @22:21:20.44 SS.hold @22:22:00.68 SS.reconfigure :default @22:22:00.73 SS.hold @22:22:00.79 Intake.select :close @22:22:00.95 ShallowSampler::Clogged in MAIN -- Intake @17psia & Outlet @15psia won't equalize after 925.0ml @22:22:00.96 SSV.dial :exhaust @22:22:01.72 Exhaust.select :open @22:22:01.84 SS.reconfigure :exhale @22:22:01.89 SS.seek 0ml @22:22:10.90 Exhaust.select :close @22:22:11.00 Sampled 925.0ml @22:22:11.02 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @22:22:11.03 Intake.select :close @22:22:11.09 CSV.dial :waste @22:22:11.26 SSV.dial CBV,avoiding: :exhaust @22:22:11.28 CS.seek 0ml @22:22:11.31
CBV.dial SSV @22:22:11.46 CSV.dial :air @22:22:12.20 CS.seek 10ml @22:22:13.23
CTV.dial :air @22:22:14.01 delay 15 seconds @22:22:29.03 SSV.dial :spit,avoiding: :exhaust @22:22:30.36 CTV.dial CSV,avoiding: :intake @22:22:30.40
SS.seek 0ml @22:22:30.52 SSV.dial CBV,avoiding: :exhaust @22:22:36.74 SSV.dial between CBV and plug @22:22:37.23 CBV.dial between SSV and waste2 @22:22:37.61 CSV.dial between air and CTV @22:22:37.93 CS.seek 8.128ml @22:22:43.12 CTV.dial CSV,avoiding: :air @22:22:43.14
CSV.dial CTV @22:22:43.53 CS.reconfigure :pressurize @22:22:43.58 CS.seek 0ml @22:22:56.68 BubblePoint: Intake @24.2psia, Outlet @1.2psia @22:22:56.69 CBV.dial SSV @22:22:57.03 SS.reconfigure :default @22:22:57.07 SS.seek 10ml @22:23:02.63 delay 15 seconds @22:23:17.76 Evacuated: Intake @23.6psia, Outlet @23.2psia @22:23:17.77 SSV.dial :spit,avoiding: :exhaust @22:23:18.81 SS.reconfigure :exhale @22:23:18.86 SS.seek 0ml @22:23:23.86 SS.reconfigure :fast @22:23:24.32 SS.seek 5ml @22:23:27.31 SSV.dial CBV,avoiding: :exhaust @22:23:27.35
CBV.dial SSV @22:23:28.66 CTV.dial :air,avoiding: :intake @22:23:29.98 CSV.dial CTV @22:23:30.02
SS.seek 10ml @22:23:31.21 CSV.dial :air @22:23:31.67 CS.reconfigure :fast @22:23:31.72 CS.seek 0ml @22:23:32.69
SSV.dial :spit,avoiding: :exhaust @22:23:34.03 SS.reconfigure :exhale @22:23:34.07 SS.seek 0ml @22:23:39.11 SS.reconfigure :fast @22:23:39.16 SS.seek 5ml @22:23:39.89 CS.stop @22:23:41.92 SSV.dial CBV,avoiding: :exhaust @22:23:41.96
CBV.dial SSV @22:23:44.26 SSV.dial :spit,avoiding: :exhaust @22:23:45.59 SS.reconfigure :default @22:23:45.64 SS.seek 0ml @22:23:48.86 SS.stop @22:23:48.90 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @22:23:48.91 Intake.select :close @22:23:48.98 CSV.dial :waste @22:23:49.12 SSV.dial CBV,avoiding: :exhaust @22:23:49.14
CBV.dial SSV @22:23:49.78 CS.seek 0ml @22:23:49.84 CSV.dial :air @22:23:50.47
CTV.dial :air @22:23:50.55 delay 15 seconds @22:23:50.70 CS.seek 10ml @22:24:05.57
SSV.dial :spit,avoiding: :exhaust @22:24:06.89 CTV.dial CSV,avoiding: :intake @22:24:06.92
SS.reconfigure :exhale @22:24:07.01 SS.seek 0ml @22:24:07.43 SSV.dial CBV,avoiding: :exhaust @22:24:15.40 SSV.dial between CBV and plug @22:24:15.87 CBV.dial between SSV and waste2 @22:24:16.27 CSV.dial between air and CTV @22:24:16.60 CS.seek 7.442ml @22:24:23.21 CTV.dial CSV,avoiding: :air @22:24:23.24
CSV.dial CTV @22:24:23.57 CS.reconfigure :pressurize @22:24:23.62 CS.seek 0ml @22:24:36.53 BubblePoint: Intake @24.1psia, Outlet @3.5psia @22:24:36.54 CBV.dial SSV @22:24:37.31 SS.reconfigure :default @22:24:37.35 SS.seek 10ml @22:24:43.00 delay 15 seconds @22:24:58.13 Evacuated: Intake @23.8psia, Outlet @23.4psia @22:24:58.14 SSV.dial :spit,avoiding: :exhaust @22:24:59.18 SS.reconfigure :exhale @22:24:59.23 SS.seek 0ml @22:25:04.22 SS.reconfigure :fast @22:25:04.27 SS.seek 5ml @22:25:07.09 SSV.dial CBV,avoiding: :exhaust @22:25:07.13
CBV.dial SSV @22:25:08.43 CTV.dial :air,avoiding: :intake @22:25:09.79 CSV.dial CTV @22:25:09.82
SS.seek 10ml @22:25:11.02 CSV.dial :air @22:25:11.48 CS.reconfigure :fast @22:25:11.53 CS.seek 0ml @22:25:12.35
SSV.dial :spit,avoiding: :exhaust @22:25:13.68 SS.reconfigure :exhale @22:25:13.73 SS.seek 0ml @22:25:18.24 CS.stop @22:25:18.74
SS.reconfigure :fast @22:25:18.78 SS.seek 5ml @22:25:21.60 SSV.dial CBV,avoiding: :exhaust @22:25:21.62
CBV.dial SSV @22:25:23.98 SSV.dial :spit,avoiding: :exhaust @22:25:25.29 SS.reconfigure :default @22:25:25.34 SS.seek 0ml @22:25:28.55 SS.stop @22:25:28.59 Lysing: Adding 2.0ml AqMeOH @22:25:28.60 CBV.dial between CSV and waste1,avoiding: :DBV @22:25:30.10 CTV.dial CSV @22:25:31.42 CSV.dial CSR @22:25:32.34 Pulling 1ml of CSR.AqMeOH @22:25:32.35 CSR.select :AqMeOH @22:25:32.46 CS.reconfigure :default @22:25:32.51 CS.seek 1ml @22:25:35.92 delay 5 seconds @22:25:40.94 CSR.select :air @22:25:41.11 CS.seek 6ml @22:25:54.74 delay 2 seconds @22:25:56.75 CSV.dial :waste2 @22:25:57.23 CS.reconfigure :fast @22:25:57.27 CS.seek 0ml @22:26:11.73 CSV.dial CSR @22:26:12.31 Pulling 2ml of CSR.AqMeOH @22:26:12.32 CSR.select :AqMeOH @22:26:12.43 CS.reconfigure :default @22:26:12.48 CS.seek 2ml @22:26:19.07 delay 5 seconds @22:26:24.09 CSR.select :air @22:26:24.25 CS.seek 4.999ml @22:26:32.74 delay 2 seconds @22:26:34.75 CSV.dial CTV @22:26:35.23 CS.seek 1ml @22:26:46.38 Before lysing Collection Puck Heater at 13.3C/31.5psia @22:26:46.40 Heating Puck.from 6, :sh1 in Collection Clamp to 70C for 10:00 @22:26:46.42 CH.seek 70C,8:00 @22:28:59.04 CH.hold 10:00,70C @22:28:59.23 Begun lysing Collection Puck Heater at 68.1C/38.4psia @22:38:59.25 Ended lysing Collection Puck Heater at 70C/37.3psia @22:38:59.27 CS.seek 0ml @22:39:02.64 CTV.dial between spare and air,avoiding: :intake @22:39:03.66 CSV.dial :air @22:39:04.11 CS.seek 5ml @22:39:17.72 CSV.dial CTV @22:39:18.18 delay 2 seconds @22:39:20.20 CSV.dial :air @22:39:20.65 delay 2 seconds @22:39:22.66 CSV.dial CTV @22:39:23.12 delay 2 seconds @22:39:25.13 CSV.dial :air @22:39:25.58 delay 2 seconds @22:39:27.61 CS.seek 0ml @22:39:41.21 Puck @82.9psia after final lysis pressurization @22:39:41.23 Recovering Lysate into Collection syringe @22:39:41.24 CSV.dial CBV @22:39:42.26 CBV.dial CSV @22:39:42.64 CS.seek 5ml @22:39:56.63 delay 5 seconds @22:40:01.65 CTV.dial :air @22:40:02.18 CS.seek 7ml @22:40:08.19 delay 5 seconds @22:40:13.21 CSV.dial :air2 @22:40:13.68 CS.seek 10ml @22:40:22.13 CSV.dial between air and CTV,avoiding: CBV @22:40:23.29 Lysing Complete @22:40:23.36 Storing sh1 puck in Collection Clamp to tube 5 @22:40:23.95 SC.reconfigure :tight @22:40:24.02 SC.seek 5 @22:40:27.60 SC.configure :limp @22:40:27.76 SE.reconfigure :place @22:40:27.84
Elbow.reconfigure :tight @22:40:27.89 SE.seek :beamBreak @22:40:27.91
Elbow.seek CC @22:40:28.27 Elbow.reconfigure :loose @22:40:28.37 Forearm.seek CC @22:40:29.41 Hand.close @22:40:30.01 CC.seek :open @22:40:30.75 SE.configure :default @22:40:30.80 SE.seek :place @22:40:34.76
Forearm.seek :retract @22:40:35.76 Elbow.reconfigure :tight @22:40:35.80 Elbow.seek 5 @22:40:37.46 Elbow.reconfigure :limp @22:40:37.51 Forearm.seek 5 @22:40:38.52 Elbow.hold @22:40:38.59 Elbow.reconfigure :tight @22:40:38.64 Hand.open @22:40:39.37 Forearm.force -20 @22:40:39.41 Forearm.seek 5 + 313 counts @22:40:39.71 Forearm.reconfigure :tight @22:40:39.76 Forearm.seek 5 + 450 counts @22:40:40.01 SE.seek :down @22:40:41.13
Forearm.reconfigure :loose @22:40:41.18 Forearm.seek :retract @22:40:42.72 SE.stop @22:40:42.76
Tranferring DA @22:40:42.77 Beginning samplePassC @22:40:42.78 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @22:40:43.41 Elbow.seek FlushPuck::Garage @22:40:45.28 Elbow.reconfigure :loose @22:40:45.39 Forearm.seek :garage @22:40:46.41 Hand.close @22:40:47.12 Forearm.seek :retract @22:40:48.10 Elbow.reconfigure :tight @22:40:48.15 Elbow.seek CC @22:40:51.08 Elbow.reconfigure :loose @22:40:51.18 Forearm.reconfigure :tight @22:40:51.23 Forearm.seek CC @22:40:52.61 CC.seek :closed @22:40:57.14 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.44 @22:40:57.21 Hand.open @22:40:57.94 Forearm.reconfigure :loose @22:40:57.99 Forearm.seek :retract @22:40:58.94 PSV.dial PSR @22:41:00.34 Pulling 1.5ml of PSR.flush @22:41:00.35 PSR.select :flush @22:41:00.48 PS.seek 1.5ml @22:41:16.60 delay 5 seconds @22:41:21.62 PSV.dial :waste1 @22:41:22.08 PS.seek 0ml @22:41:38.13 PSV.dial PSR @22:41:38.60 PSR.select :air @22:41:38.71 PS.seek 2.5ml @22:42:05.04 PRV.dial CBV,avoiding: :ab2 @22:42:05.06
CBV.dial PRV @22:42:07.27 PSV.dial :mixloop @22:42:08.31 CTV.dial :air,avoiding: :intake @22:42:08.47 PTV.dial :PRVmixing @22:42:09.81 PS.seek 0ml @22:42:36.13 PSV.dial :air @22:42:36.61 PS.seek 2.5ml @22:43:03.04 PSV.dial :mixloop @22:43:03.51 PS.seek 0ml @22:43:29.91 PSV.dial :air @22:43:30.39 PS.seek 2.5ml @22:43:56.69 PSV.dial :mixloop @22:43:57.17 PS.seek 0ml @22:44:23.44 Garaging FlushPuck in Collection Clamp @22:44:23.51 Elbow.reconfigure :tight @22:44:23.56 Elbow.seek CC @22:44:23.86 Elbow.reconfigure :loose @22:44:23.96 Forearm.seek CC @22:44:25.00 Hand.close @22:44:25.63 CC.seek :open @22:44:30.27 Forearm.seek :retract @22:44:31.29 Elbow.reconfigure :tight @22:44:31.34 Elbow.seek :garage @22:44:34.29 Elbow.reconfigure :loose @22:44:34.39 Forearm.reconfigure :tight @22:44:34.44 Forearm.seek :garage @22:44:35.53 Hand.open @22:44:36.19 Forearm.reconfigure :loose @22:44:36.23 Forearm.seek :retract @22:44:37.28 PSV.dial :mixloop @22:44:37.40 PTV.dial :PRVmixing @22:44:37.52 PRV.dial CBV @22:44:37.54
CBV.dial PRV @22:44:37.68 CTV.dial :air,avoiding: :intake @22:44:37.70 Loading lyfil puck from tube 6 into Collection Clamp @22:44:38.36 SC.reconfigure :tight @22:44:38.41 SC.seek 6 @22:44:41.93 SC.configure :limp @22:44:42.15 Elbow.reconfigure :tight @22:44:42.20 Elbow.seek 6 @22:44:44.14 Forearm.reconfigure :tight @22:44:44.19 Forearm.seek 6 + 450 counts @22:44:45.31 SE.reconfigure :pick @22:44:45.37 SE.seek :beamBreak @22:44:46.38 SE.configure :default @22:44:46.54 Elbow.seek 6 @22:44:46.73 Forearm.seek 6 + 300 counts @22:44:46.88 Forearm.reconfigure :loose @22:44:46.92 Hand.close @22:44:47.52 SE.seek :down @22:44:47.71
Forearm.seek :retract @22:44:48.23 SE.stop @22:44:48.65
Elbow.seek CC @22:44:50.28 Elbow.reconfigure :loose @22:44:50.38 Forearm.reconfigure :tight @22:44:50.43 Forearm.seek CC @22:44:51.64 CC.seek :closed @22:44:56.16 Collection Clamp onSpring/offSpring for 13/27 samples, on/off torque 2.15 @22:44:56.17 Hand.open @22:44:56.81 Forearm.reconfigure :loose @22:44:56.86 Forearm.seek :retract @22:44:57.82 CTV.dial CSV,avoiding: :intake @22:44:59.16 CSV.dial CTV @22:44:59.48 PS.seek 2.5ml @22:45:25.91 CS.seek 4.5ml @22:45:40.81 delay 8 seconds @22:45:48.84 PSV.dial :air @22:45:49.30 delay 2 seconds @22:45:51.32 PSV.dial :waste2 @22:45:52.06 PS.seek 0ml @22:46:18.47 CBV.dial between PRV and waste1 @22:46:18.80 CSV.dial :air @22:46:19.26 CS.seek 10ml @22:46:34.22 CSV.dial CTV @22:46:34.68 CS.seek 0ml @22:47:01.19 CBV.dial :waste1 @22:47:01.52 delay 5 seconds @22:47:06.54 CTV.dial :air,avoiding: :intake @22:47:07.95 Storing lyfil puck in Collection Clamp to tube 5 @22:47:08.13 SC.reconfigure :tight @22:47:08.18 SC.seek 5 @22:47:11.72 SC.configure :limp @22:47:11.94 Elbow.reconfigure :tight @22:47:11.99 Elbow.seek 5 @22:47:13.75 Forearm.reconfigure :tight @22:47:13.80 Forearm.seek 5 + 450 counts @22:47:14.82 SE.reconfigure :pick @22:47:14.88 SE.seek :beamBreak @22:47:17.94 SE.configure :default @22:47:18.54 Elbow.reconfigure :limp @22:47:18.59 Forearm.reconfigure :loose @22:47:18.64 Forearm.seek 5 @22:47:18.86 Elbow.hold @22:47:18.93 Elbow.reconfigure :tight @22:47:19.03 SE.seek 0.18 Pucks below Top Plate @22:47:19.45 Forearm.force -20 @22:47:19.50 Forearm.seek 5 + 302 counts @22:47:19.79 Forearm.reconfigure :tight @22:47:19.84 Forearm.seek 5 + 450 counts @22:47:20.04 SE.seek 1.18 Pucks below Top Plate @22:47:20.54 Forearm.reconfigure :loose @22:47:20.59 Forearm.seek :retract @22:47:21.53 Elbow.seek CC @22:47:23.25 Elbow.reconfigure :loose @22:47:23.35 Forearm.seek CC @22:47:24.37 Hand.close @22:47:25.00 CC.seek :open @22:47:29.73 Forearm.seek :retract @22:47:30.74 Elbow.reconfigure :tight @22:47:30.78 Elbow.seek 5 @22:47:32.54 Elbow.reconfigure :limp @22:47:32.59 Forearm.seek 5 @22:47:33.64 Elbow.hold @22:47:33.71 Elbow.reconfigure :tight @22:47:33.76 Hand.open @22:47:34.87 Forearm.force -20 @22:47:34.98 Forearm.seek 5 + 306 counts @22:47:35.30 Forearm.reconfigure :tight @22:47:35.35 Forearm.seek 5 + 450 counts @22:47:35.60 SE.seek :down @22:47:36.73
Forearm.reconfigure :loose @22:47:36.78 Forearm.seek :retract @22:47:38.21 SE.stop @22:47:38.25
PSV.dial :air @22:47:39.01 PS.seek 2.5ml @22:48:05.44 delay 1 second @22:48:06.45 PSV.dial :mixloop @22:48:06.91 delay 1 second @22:48:07.92 PSV.dial :air @22:48:08.39 delay 1 second @22:48:09.41 PSV.dial :mixloop @22:48:09.88 delay 1 second @22:48:10.89 PSV.dial :air @22:48:11.36 delay 1 second @22:48:12.38 PS.seek 0ml @22:48:38.66 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @22:48:38.85 Elbow.seek FlushPuck::Garage @22:48:40.60 Elbow.reconfigure :loose @22:48:40.70 Forearm.seek :garage @22:48:41.73 Hand.close @22:48:42.49 Forearm.seek :retract @22:48:43.47 Elbow.reconfigure :tight @22:48:43.52 Elbow.seek PC @22:48:44.79 Elbow.reconfigure :loose @22:48:44.89 Forearm.reconfigure :tight @22:48:44.94 Forearm.seek PC @22:48:46.67 PC.seek :closed @22:48:51.35 Processing Clamp onSpring/offSpring for 16/26 samples, on/off torque 1.64 @22:48:51.42 Hand.open @22:48:52.06 Forearm.reconfigure :loose @22:48:52.11 Forearm.seek :retract @22:48:53.06 PRV.dial :air @22:48:53.52 PSV.dial :mixloop @22:48:54.05 PS.seek 0.3ml @22:48:58.00 PSV.dial PSR @22:48:59.05 Pulling 0.4ml of PSR.air @22:48:59.06 PSR.select :air @22:48:59.08 PS.seek 0.7ml @22:49:03.97 delay 3 seconds @22:49:06.99 PSR.select :air @22:49:07.01 PS.seek 2.5ml @22:49:26.16 delay 3 seconds @22:49:29.17 PSV.dial :mixloop @22:49:30.22 PTV.dial :mixing @22:49:30.97 PBV.dial :waste2 @22:49:31.94 PS.seek 0ml @22:49:58.25 Garaging FlushPuck in Processing Clamp @22:49:58.32 Elbow.reconfigure :tight @22:49:58.37 Elbow.seek PC @22:49:58.48 Elbow.reconfigure :loose @22:49:58.59 Forearm.seek PC @22:49:59.56 Hand.close @22:50:00.13 PC.seek :open @22:50:04.87 Forearm.seek :retract @22:50:05.81 Elbow.reconfigure :tight @22:50:05.86 Elbow.seek :garage @22:50:07.24 Elbow.reconfigure :loose @22:50:07.34 Forearm.reconfigure :tight @22:50:07.39 Forearm.seek :garage @22:50:08.42 Hand.open @22:50:09.56 Forearm.reconfigure :loose @22:50:09.61 Forearm.seek :retract @22:50:10.60 Loading da puck from tube 6 into Processing Clamp @22:50:10.83 SC.reconfigure :tight @22:50:10.88 SC.seek 6 @22:50:14.40 SC.configure :limp @22:50:14.61 Elbow.reconfigure :tight @22:50:14.66 Elbow.seek 6 @22:50:16.53 Forearm.reconfigure :tight @22:50:16.57 Forearm.seek 6 + 450 counts @22:50:17.58 SE.reconfigure :pick @22:50:17.63 SE.seek :beamBreak @22:50:18.80 SE.configure :default @22:50:18.96 Elbow.seek 6 @22:50:19.14 Forearm.seek 6 + 300 counts @22:50:19.42 Forearm.reconfigure :loose @22:50:19.46 Hand.close @22:50:20.14 SE.seek :down @22:50:20.33
Forearm.seek :retract @22:50:20.87 SE.stop @22:50:21.27
Elbow.seek PC @22:50:23.80 Elbow.reconfigure :loose @22:50:23.90 Forearm.reconfigure :tight @22:50:23.95 Forearm.seek PC @22:50:25.16 PC.seek :closed @22:50:29.79 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.63 @22:50:29.80 Hand.open @22:50:30.43 Forearm.reconfigure :loose @22:50:30.48 Forearm.seek :retract @22:50:31.44 Heating Puck.from 6, :da in Processing Clamp to 28C for 2:00:00 @22:50:31.46 PH.seek 28C,5:00 @22:51:11.59 PH.hold 2:00:00,28C @22:51:11.64 delay 1:30 @22:52:41.68 PSR.select :air @22:52:41.70 PSV.dial PSR @22:52:42.75 Pulling 0.5ml of PSR.wash PSR.select :wash @22:52:42.87 PS.seek 0.5ml @22:52:49.25 delay 5 seconds @22:52:54.27 PSR.select :air @22:52:54.39 PS.seek 2ml @22:53:10.59 PTV.dial :puck @22:53:11.34 PSV.dial PTV @22:53:12.10 PBV.dial :waste1 @22:53:13.50 PS.seek 1.5ml @22:53:19.40 delay 3 seconds @22:53:22.42 PBV.dial between PSV and waste1 @22:53:22.78 PS.seek 0ml @22:53:39.00 delay 5:00 @22:58:39.01 recoverPSRreagent08 @22:58:39.02 PTV.dial :puck @22:58:39.10 PBV.dial :waste1 @22:58:39.48 delay 30 seconds @22:59:09.50 PBV.dial between PSV and waste1 @22:59:09.86 PSR.select :air @22:59:09.88 PSV.dial PSR @22:59:10.65 PS.reconfigure :fast @22:59:10.70 PS.seek 2.5ml @22:59:34.36 PSV.dial PTV @22:59:35.13 delay 5 seconds @22:59:40.15 PSV.dial PSR @22:59:40.90 delay 5 seconds @22:59:45.92 PSV.dial PTV @22:59:46.68 delay 5 seconds @22:59:51.70 PSV.dial :air @22:59:52.48 PS.seek 0ml @23:00:15.77 PTV.dial :PRVmixing,via: PRV @23:00:17.10 PSV.dial :mixloop @23:00:17.57 PRV.dial :ab1 @23:00:18.32 PS.reconfigure :slow @23:00:18.37 PS.seek 0.15ml @23:00:21.99 delay 2 seconds @23:00:24.01 CBV.dial PRV @23:00:24.03
PRV.dial CBV @23:00:24.61 PS.seek 0.35ml @23:00:29.24 delay 2 seconds @23:00:31.25 CBV.dial between PRV and PSV @23:00:31.56 PRV.dial :air @23:00:32.03 PS.seek 0.9ml @23:00:43.67 PRV.dial :waste2 @23:00:44.42 PS.seek 0.5ml @23:00:53.08 addPMLReagent08 @23:00:53.09 PSV.dial :air @23:00:53.55 PS.reconfigure :default @23:00:53.60 PS.seek 2ml @23:01:09.77 PSV.dial :PML @23:01:10.24 PTV.dial :mixing @23:01:11.36 PBV.dial :waste1 @23:01:11.83 PS.seek 1.85ml @23:01:14.20 PBV.dial between PSV and waste1 @23:01:14.57 PS.seek 0ml @23:01:34.30 delay 2 seconds @23:01:36.32 Beginning samplePassC @23:01:36.33 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @23:01:36.52 Elbow.reconfigure :tight @23:01:36.56 Elbow.seek FlushPuck::Garage @23:01:37.95 Elbow.reconfigure :loose @23:01:38.05 Forearm.seek :garage @23:01:38.99 Hand.close @23:01:39.74 Forearm.seek :retract @23:01:40.71 Elbow.reconfigure :tight @23:01:40.76 Elbow.seek CC @23:01:43.59 Elbow.reconfigure :loose @23:01:43.69 Forearm.reconfigure :tight @23:01:43.74 Forearm.seek CC @23:01:44.97 CC.seek :closed @23:01:49.55 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.48 @23:01:49.62 Hand.open @23:01:50.30 Forearm.reconfigure :loose @23:01:50.35 Forearm.seek :retract @23:01:51.28 PSV.dial PSR @23:01:52.47 Pulling 1.5ml of PSR.flush @23:01:52.48 PSR.select :flush @23:01:52.99 PS.seek 1.5ml @23:02:09.14 delay 5 seconds @23:02:14.16 PSV.dial :waste1 @23:02:14.65 PS.seek 0ml @23:02:30.73 PSV.dial PSR @23:02:31.20 PSR.select :air @23:02:31.32 PS.seek 2.5ml @23:02:57.65 PRV.dial CBV,avoiding: :ab2 @23:02:57.67
CBV.dial PRV @23:02:59.30 PSV.dial :mixloop @23:03:00.35 CTV.dial :air,avoiding: :intake @23:03:00.36 PTV.dial :PRVmixing @23:03:01.15 PS.seek 0ml @23:03:27.55 PSV.dial :air @23:03:28.02 PS.seek 2.5ml @23:03:54.39 PSV.dial :mixloop @23:03:54.88 PS.seek 0ml @23:04:21.20 PSV.dial :air @23:04:21.68 PS.seek 2.5ml @23:04:48.23 PSV.dial :mixloop @23:04:48.71 PS.seek 0ml @23:05:14.98 Garaging FlushPuck in Collection Clamp @23:05:15.05 Elbow.reconfigure :tight @23:05:15.10 Elbow.seek CC @23:05:15.42 Elbow.reconfigure :loose @23:05:15.52 Forearm.seek CC @23:05:16.53 Hand.close @23:05:17.11 CC.seek :open @23:05:21.76 Forearm.seek :retract @23:05:22.75 Elbow.reconfigure :tight @23:05:22.80 Elbow.seek :garage @23:05:25.64 Elbow.reconfigure :loose @23:05:25.74 Forearm.reconfigure :tight @23:05:25.79 Forearm.seek :garage @23:05:26.82 Hand.open @23:05:27.55 Forearm.reconfigure :loose @23:05:27.59 Forearm.seek :retract @23:05:28.63 delayUntil "23:09:36.31" @23:05:28.66 Whole Cell Archive sampling 1000ml @23:05:28.68 Loading wcr puck from tube 6 into Collection Clamp @23:05:29.16 Elbow.reconfigure :tight @23:05:29.21 Elbow.seek 6 @23:05:31.19 Forearm.reconfigure :tight @23:05:31.24 Forearm.seek 6 + 450 counts @23:05:32.58 SE.reconfigure :pick @23:05:32.64 SE.seek :beamBreak @23:05:33.78 SE.configure :default @23:05:33.95 Elbow.seek 6 @23:05:34.14 Forearm.seek 6 + 300 counts @23:05:34.28 Forearm.reconfigure :loose @23:05:34.33 Hand.close @23:05:35.00 SE.seek :down @23:05:35.21 Forearm.seek :retract @23:05:35.83 SE.stop @23:05:36.19 Elbow.seek CC @23:05:37.82 Elbow.reconfigure :loose @23:05:37.92 Forearm.reconfigure :tight @23:05:37.98 Forearm.seek CC @23:05:39.22 CC.seek :closed @23:05:43.74 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.29 @23:05:43.75 Hand.open @23:05:44.42 Forearm.reconfigure :loose @23:05:44.48 Forearm.seek :retract @23:05:45.47 collectSample 1000ml w/bubble point of 35psi @23:05:45.49 Priming 7ml @23:05:45.50 Intake.select :close @23:05:45.51 Exhaust.select :close @23:05:45.52 SS.hold @23:05:45.74 Calibrating pressure sensors @23:05:45.75 CTV.dial :air @23:05:45.76 SSV.dial :spit,avoiding: :exhaust @23:05:45.85 SS.reconfigure :fast @23:05:45.90 SS.seek 5ml @23:05:48.65 SSV.dial CBV @23:05:48.67 CBV.dial SSV @23:05:50.07 delay 3.8 seconds @23:05:54.00 Sensor limits: Intake max 101.9psia; Outlet max 93.6psia @23:05:54.01 SSV.dial :spit @23:05:55.32 SS.seek 0ml @23:05:58.24 SSV.dial CBV @23:05:59.58 CTV.dial :intake @23:06:00.34 SSV.dial CBV @23:06:00.39 CBV.dial SSV @23:06:00.51 Allowing 40s for 7.0ml of remaining 7.0ml @23:06:00.52 Intake.select :open @23:06:02.69 Intake @14.5psia, vacuum from 9.0 to 12.0psi @23:06:02.71 SS.reconfigure :inhaling 2.20ml/s @23:06:02.77 SS.seek 7ml @23:06:09.66 Stroke completed -- waiting for pressure to equalize ... @23:06:09.67 SS.reconfigure :exhale @23:06:09.72 SS.hold @23:06:26.48 Intake.select :close @23:06:26.64 SSV.dial :exhaust @23:06:27.41 Exhaust.select :open @23:06:27.53 SS.seek 0ml @23:06:31.63 Exhaust.select :close @23:06:31.74 Sampling 1000.0ml @23:06:31.76 Intake.select :close @23:06:31.77 Exhaust.select :close @23:06:31.78 SS.reconfigure :default @23:06:31.83 SS.hold @23:06:32.04 CTV.dial :intake @23:06:32.13 SSV.dial CBV @23:06:32.15 CBV.dial SSV @23:06:32.94 Allowing 130s for 25.0ml of remaining 1000.0ml @23:06:32.96 Intake.select :open @23:06:35.12 Intake @15.5psia, vacuum from 10.0 to 13.0psi @23:06:35.14 SS.reconfigure :inhaling 2.20ml/s @23:06:35.19 SS.seek 25ml @23:06:41.96 Vacuum too high -- waiting up to 40 seconds @23:06:41.98 SS.reconfigure :exhale @23:06:42.03 SS.hold @23:06:50.07 Intake @18.8psia, vacuum from 10.0 to 13.0psi @23:06:50.09 SS.reconfigure :inhaling 1.76ml/s @23:06:50.14 SS.seek 25ml @23:06:52.21 Vacuum too high -- waiting up to 40 seconds @23:06:52.23 SS.reconfigure :exhale @23:06:52.29 SS.hold @23:06:55.00 Intake @19.2psia, vacuum from 10.0 to 13.0psi @23:06:55.02 SS.reconfigure :inhaling 1.41ml/s @23:06:55.07 SS.seek 25ml @23:06:57.15 Vacuum too high -- waiting up to 40 seconds @23:06:57.16 SS.reconfigure :exhale @23:06:57.22 SS.hold @23:06:59.30 Intake @19.3psia, vacuum from 10.0 to 13.0psi @23:06:59.32 SS.reconfigure :inhaling 1.13ml/s @23:06:59.37 SS.seek 25ml @23:07:01.65 Vacuum too high -- waiting up to 40 seconds @23:07:01.67 SS.reconfigure :exhale @23:07:01.72 SS.hold @23:07:03.84 Intake @19.4psia, vacuum from 10.0 to 13.0psi @23:07:03.86 SS.reconfigure :inhaling 0.91ml/s @23:07:03.91 SS.seek 25ml @23:07:06.65 Vacuum too high -- waiting up to 40 seconds @23:07:06.67 SS.reconfigure :exhale @23:07:06.72 SS.hold @23:07:08.44 Intake @19.5psia, vacuum from 10.0 to 13.0psi @23:07:08.45 SS.reconfigure :inhaling 0.73ml/s @23:07:08.50 SS.seek 25ml @23:07:12.34 Vacuum too high -- waiting up to 40 seconds @23:07:12.35 SS.reconfigure :exhale @23:07:12.41 SS.hold @23:07:14.04 SS.reconfigure :inhaling 0.59ml/s @23:07:14.09 SS.seek 25ml @23:07:21.59 Vacuum too high -- waiting up to 40 seconds @23:07:21.60 SS.reconfigure :exhale @23:07:21.66 SS.hold @23:07:22.99 Intake @19.0psia, vacuum from 10.0 to 13.0psi @23:07:23.01 SS.reconfigure :inhaling 0.47ml/s @23:07:23.06 SS.seek 25ml @23:07:26.87 Stroke completed -- waiting for pressure to equalize ... @23:07:26.88 SS.reconfigure :exhale @23:07:26.93 SS.hold @23:08:01.68 Intake.select :close @23:08:01.84 SSV.dial :exhaust @23:08:02.60 Exhaust.select :open @23:08:02.71 SS.seek 0ml @23:08:11.64 Exhaust.select :close @23:08:11.75 SSV.dial CBV @23:08:11.78 CBV.dial SSV @23:08:12.55 Allowing 130s for 25.0ml of remaining 975.0ml @23:08:12.56 Intake.select :open @23:08:14.73 Intake @16.1psia, vacuum from 10.0 to 13.0psi @23:08:14.75 SS.reconfigure :inhaling 0.47ml/s @23:08:14.80 SS.seek 25ml @23:09:10.44 Stroke completed -- waiting for pressure to equalize ... @23:09:10.45 SS.reconfigure :exhale @23:09:10.50 SS.hold @23:09:36.32 recoverPRVreagent08 @23:09:36.33 PTV.dial :mixing @23:09:37.09 PBV.dial :waste1 @23:09:37.46 delay 30 seconds @23:09:47.48 Intake.select :close @23:09:48.02 SSV.dial :exhaust @23:09:48.86 Exhaust.select :open @23:09:48.98 SS.seek 0ml @23:09:58.03 Exhaust.select :close @23:09:58.15 SSV.dial CBV @23:09:58.18 CBV.dial SSV @23:09:58.95 Allowing 130s for 25.0ml of remaining 950.0ml @23:09:58.97 Intake.select :open @23:10:01.14 Intake @16.0psia, vacuum from 10.0 to 13.0psi @23:10:01.16 SS.reconfigure :inhaling 0.47ml/s @23:10:01.21 SS.seek 25ml @23:10:07.48 PBV.dial between PSV and waste1 @23:10:07.87 PSV.dial :air @23:10:08.48 PS.reconfigure :fast @23:10:08.54 PS.seek 2.5ml @23:10:32.66 PSV.dial :mixloop @23:10:33.12 delay 2 seconds @23:10:35.14 PSV.dial :air @23:10:35.60 delay 2 seconds @23:10:37.62 PSV.dial :mixloop @23:10:38.10 delay 2 seconds @23:10:40.12 PSV.dial :air @23:10:40.73 PS.seek 0ml @23:10:56.23 Stroke completed -- waiting for pressure to equalize ... @23:10:56.24 SS.reconfigure :exhale @23:10:56.29 SS.hold @23:11:04.26 washCycleDA #1 of 2 @23:11:04.27 PSR.select :air @23:11:04.28 PSV.dial PSR @23:11:05.63 Pulling 0.5ml of PSR.wash @23:11:05.64 PSR.select :wash @23:11:05.76 PS.reconfigure :default @23:11:05.81 PS.seek 0.5ml @23:11:11.82 delay 5 seconds @23:11:16.83 PSR.select :air @23:11:16.95 PS.seek 2ml @23:11:26.95 Intake.select :close @23:11:27.11 SSV.dial :exhaust @23:11:27.87 Exhaust.select :open @23:11:27.99 SS.seek 0ml @23:11:33.10 PTV.dial :puck @23:11:33.85 PSV.dial PTV @23:11:34.62 PBV.dial :waste1 @23:11:35.05 PS.seek 1.5ml @23:11:36.70 Exhaust.select :close @23:11:36.81 SSV.dial CBV @23:11:36.84 CBV.dial SSV @23:11:37.61 Allowing 130s for 25.0ml of remaining 925.0ml @23:11:37.62 Intake.select :open @23:11:39.79 Intake @16.1psia, vacuum from 10.0 to 13.0psi @23:11:39.81 SS.reconfigure :inhaling 0.47ml/s @23:11:39.86 SS.seek 25ml @23:11:41.04 delay 3 seconds @23:11:44.06 PBV.dial between PSV and waste1 @23:11:44.57 PS.seek 0ml @23:12:00.66 delay 2:00 @23:12:35.35 Stroke completed -- waiting for pressure to equalize ... @23:12:35.37 SS.reconfigure :exhale @23:12:35.42 SS.hold @23:13:06.17 Intake.select :close @23:13:06.33 SSV.dial :exhaust @23:13:07.09 Exhaust.select :open @23:13:07.21 SS.seek 0ml @23:13:16.28 Exhaust.select :close @23:13:16.39 SSV.dial CBV @23:13:16.42 CBV.dial SSV @23:13:17.22 Allowing 130s for 25.0ml of remaining 900.0ml @23:13:17.23 Intake.select :open @23:13:19.40 Intake @16.2psia, vacuum from 10.0 to 13.0psi @23:13:19.42 SS.reconfigure :inhaling 0.47ml/s @23:13:19.47 SS.seek 25ml @23:14:00.69 recoverPSRreagent08 @23:14:00.70 PTV.dial :puck @23:14:00.77 PBV.dial :waste1 @23:14:01.16 delay 30 seconds @23:14:14.46 Stroke completed -- waiting for pressure to equalize ... @23:14:14.47 SS.reconfigure :exhale @23:14:14.52 SS.hold @23:14:31.18 PBV.dial between PSV and waste1 @23:14:31.55 PSR.select :air @23:14:31.57 PSV.dial PSR @23:14:32.34 PS.reconfigure :fast @23:14:32.39 PS.seek 2.5ml @23:14:40.17 Intake.select :close @23:14:40.32 SSV.dial :exhaust @23:14:41.09 Exhaust.select :open @23:14:41.21 SS.seek 0ml @23:14:50.27 Exhaust.select :close @23:14:50.38 SSV.dial CBV @23:14:50.43 CBV.dial SSV @23:14:51.17 Allowing 130s for 25.0ml of remaining 875.0ml @23:14:51.18 Intake.select :open @23:14:53.35 Intake @16.1psia, vacuum from 10.0 to 13.0psi @23:14:53.37 SS.reconfigure :inhaling 0.47ml/s @23:14:53.42 SS.seek 25ml @23:14:56.50 PSV.dial PTV @23:14:57.29 delay 5 seconds @23:15:02.31 PSV.dial PSR @23:15:03.07 delay 5 seconds @23:15:08.09 PSV.dial PTV @23:15:08.85 delay 5 seconds @23:15:13.87 PSV.dial :air @23:15:14.63 PS.seek 0ml @23:15:37.97 washCycleDA #2 of 2 @23:15:37.98 PSR.select :air @23:15:38.00 PSV.dial PSR @23:15:39.35 Pulling 0.5ml of PSR.wash @23:15:39.36 PSR.select :wash @23:15:39.48 PS.reconfigure :default @23:15:39.52 PS.seek 0.5ml @23:15:45.44 delay 5 seconds @23:15:48.26 Stroke completed -- waiting for pressure to equalize ... @23:15:48.27 SS.reconfigure :exhale @23:15:48.32 SS.hold @23:15:50.45 PSR.select :air @23:15:50.65 PS.seek 2ml @23:16:06.79 PTV.dial :puck @23:16:06.86 PSV.dial PTV @23:16:07.61 PBV.dial :waste1 @23:16:08.05 PS.seek 1.5ml @23:16:13.17 Intake.select :close @23:16:13.33 SSV.dial :exhaust @23:16:13.97 delay 3 seconds @23:16:14.56 Exhaust.select :open @23:16:14.68 SS.seek 0ml @23:16:16.99 PBV.dial between PSV and waste1 @23:16:17.37 PS.seek 0ml @23:16:23.43 Exhaust.select :close @23:16:23.54 SSV.dial CBV @23:16:23.59 CBV.dial SSV @23:16:24.35 Allowing 130s for 25.0ml of remaining 850.0ml @23:16:24.37 Intake.select :open @23:16:26.54 Intake @16.2psia, vacuum from 10.0 to 13.0psi @23:16:26.56 SS.reconfigure :inhaling 0.47ml/s @23:16:26.61 SS.seek 25ml @23:16:33.47 delay 2:00 @23:17:22.18 Stroke completed -- waiting for pressure to equalize ... @23:17:22.19 SS.reconfigure :exhale @23:17:22.24 SS.hold @23:17:45.20 Intake.select :close @23:17:45.36 SSV.dial :exhaust @23:17:46.13 Exhaust.select :open @23:17:46.25 SS.seek 0ml @23:17:55.11 Exhaust.select :close @23:17:55.22 SSV.dial CBV @23:17:55.25 CBV.dial SSV @23:17:56.04 Allowing 130s for 25.0ml of remaining 825.0ml @23:17:56.05 Intake.select :open @23:17:58.22 Intake @16.2psia, vacuum from 10.0 to 13.0psi @23:17:58.24 SS.reconfigure :inhaling 0.47ml/s @23:17:58.29 SS.seek 25ml @23:18:33.49 recoverPSRreagent08 @23:18:33.50 PTV.dial :puck @23:18:33.58 PBV.dial :waste1 @23:18:33.95 delay 30 seconds @23:18:53.72 Stroke completed -- waiting for pressure to equalize ... @23:18:53.74 SS.reconfigure :exhale @23:18:53.79 SS.hold @23:19:03.97 PBV.dial between PSV and waste1 @23:19:04.35 PSR.select :air @23:19:04.37 PSV.dial PSR @23:19:05.14 PS.reconfigure :fast @23:19:05.19 PS.seek 2.5ml @23:19:16.23 Intake.select :close @23:19:16.38 SSV.dial :exhaust @23:19:17.15 Exhaust.select :open @23:19:17.26 SS.seek 0ml @23:19:26.18 Exhaust.select :close @23:19:26.29 SSV.dial CBV @23:19:26.32 CBV.dial SSV @23:19:27.08 Allowing 130s for 25.0ml of remaining 800.0ml @23:19:27.10 Intake.select :open @23:19:29.12 PSV.dial PTV @23:19:29.27 Intake @16.3psia, vacuum from 10.0 to 13.0psi @23:19:29.29 SS.reconfigure :inhaling 0.47ml/s @23:19:29.33 SS.seek 25ml @23:19:29.89 delay 5 seconds @23:19:34.91 PSV.dial PSR @23:19:35.67 delay 5 seconds @23:19:40.69 PSV.dial PTV @23:19:41.47 delay 5 seconds @23:19:46.49 PSV.dial :air @23:19:47.25 PS.seek 0ml @23:20:10.81 getPMLReagent08 @23:20:10.82 PRV.dial :air @23:20:11.29 delay 5 seconds @23:20:16.32 PTV.dial :PRVmixing @23:20:17.66 delay 5 seconds @23:20:22.68 PRV.dial :ab2 @23:20:23.14 PTV.dial :PRVmixing @23:20:23.22 PSV.dial :mixloop @23:20:23.69 PS.reconfigure :default @23:20:24.19 PS.seek 0.3ml @23:20:24.94 Stroke completed -- waiting for pressure to equalize ... @23:20:24.95 SS.reconfigure :exhale @23:20:25.00 SS.hold @23:20:28.12 delay 2 seconds @23:20:30.14 PRV.dial :air @23:20:30.68 PS.seek 0.55ml @23:20:34.00 addPMLReagent08 @23:20:34.01 PSV.dial :air @23:20:34.66 PS.seek 2ml @23:20:47.82 Intake.select :close @23:20:47.98 SSV.dial :exhaust @23:20:48.75 Exhaust.select :open @23:20:48.88 SS.seek 0ml @23:20:50.28 PSV.dial :PML @23:20:50.73 PTV.dial :mixing @23:20:51.50 PBV.dial :waste1 @23:20:51.93 PS.seek 1.85ml @23:20:54.34 PBV.dial between PSV and waste1 @23:20:54.72 PS.seek 0ml @23:20:57.75 Exhaust.select :close @23:20:57.87 SSV.dial CBV @23:20:57.89 CBV.dial SSV @23:20:58.66 Allowing 130s for 25.0ml of remaining 775.0ml @23:20:58.68 Intake.select :open @23:21:00.85 Intake @16.2psia, vacuum from 10.0 to 13.0psi @23:21:00.87 SS.reconfigure :inhaling 0.47ml/s @23:21:00.92 SS.seek 25ml @23:21:14.37 delay 2 seconds @23:21:16.39 delay 8:00 @23:21:56.36 Stroke completed -- waiting for pressure to equalize ... @23:21:56.37 SS.reconfigure :exhale @23:21:56.42 SS.hold @23:22:18.96 Intake.select :close @23:22:19.12 SSV.dial :exhaust @23:22:19.88 Exhaust.select :open @23:22:19.99 SS.seek 0ml @23:22:29.06 Exhaust.select :close @23:22:29.17 SSV.dial CBV @23:22:29.20 CBV.dial SSV @23:22:29.99 Allowing 130s for 25.0ml of remaining 750.0ml @23:22:30.00 Intake.select :open @23:22:32.17 Intake @16.3psia, vacuum from 10.0 to 13.0psi @23:22:32.19 SS.reconfigure :inhaling 0.47ml/s @23:22:32.24 SS.seek 25ml @23:23:27.29 Stroke completed -- waiting for pressure to equalize ... @23:23:27.31 SS.reconfigure :exhale @23:23:27.36 SS.hold @23:23:51.90 Intake.select :close @23:23:52.06 SSV.dial :exhaust @23:23:52.83 Exhaust.select :open @23:23:52.95 SS.seek 0ml @23:24:01.81 Exhaust.select :close @23:24:01.93 SSV.dial CBV @23:24:01.97 CBV.dial SSV @23:24:02.72 Allowing 130s for 25.0ml of remaining 725.0ml @23:24:02.73 Intake.select :open @23:24:04.90 Intake @16.4psia, vacuum from 10.0 to 13.0psi @23:24:04.92 SS.reconfigure :inhaling 0.47ml/s @23:24:04.97 SS.seek 25ml @23:25:00.50 Stroke completed -- waiting for pressure to equalize ... @23:25:00.51 SS.reconfigure :exhale @23:25:00.56 SS.hold @23:25:27.30 Intake.select :close @23:25:27.46 SSV.dial :exhaust @23:25:28.24 Exhaust.select :open @23:25:28.36 SS.seek 0ml @23:25:37.40 Exhaust.select :close @23:25:37.51 SSV.dial CBV @23:25:37.53 CBV.dial SSV @23:25:38.31 Allowing 130s for 25.0ml of remaining 700.0ml @23:25:38.32 Intake.select :open @23:25:40.49 Intake @16.3psia, vacuum from 10.0 to 13.0psi @23:25:40.51 SS.reconfigure :inhaling 0.47ml/s @23:25:40.56 SS.seek 25ml @23:26:35.90 Stroke completed -- waiting for pressure to equalize ... @23:26:35.91 SS.reconfigure :exhale @23:26:35.96 SS.hold @23:27:02.57 Intake.select :close @23:27:03.19 SSV.dial :exhaust @23:27:03.97 Exhaust.select :open @23:27:04.08 SS.seek 0ml @23:27:12.93 Exhaust.select :close @23:27:13.04 SSV.dial CBV @23:27:13.09 CBV.dial SSV @23:27:13.83 Allowing 130s for 25.0ml of remaining 675.0ml @23:27:13.84 Intake.select :open @23:27:16.01 Intake @16.4psia, vacuum from 10.0 to 13.0psi @23:27:16.03 SS.reconfigure :inhaling 0.47ml/s @23:27:16.08 SS.seek 25ml @23:28:11.13 Stroke completed -- waiting for pressure to equalize ... @23:28:11.15 SS.reconfigure :exhale @23:28:11.19 SS.hold @23:28:39.80 Intake.select :close @23:28:39.96 SSV.dial :exhaust @23:28:40.73 Exhaust.select :open @23:28:40.85 SS.seek 0ml @23:28:49.70 Exhaust.select :close @23:28:49.81 SSV.dial CBV @23:28:49.83 CBV.dial SSV @23:28:50.60 Allowing 130s for 25.0ml of remaining 650.0ml @23:28:50.62 Intake.select :open @23:28:52.79 Intake @16.4psia, vacuum from 10.0 to 13.0psi @23:28:52.81 SS.reconfigure :inhaling 0.47ml/s @23:28:52.86 SS.seek 25ml @23:29:16.41 recoverPRVreagent08 @23:29:16.42 PTV.dial :mixing @23:29:16.49 PBV.dial :waste1 @23:29:16.86 delay 30 seconds @23:29:46.89 PBV.dial between PSV and waste1 @23:29:47.25 PSV.dial :air @23:29:47.73 PS.reconfigure :fast @23:29:47.78 PS.seek 2.5ml @23:29:48.38 Stroke completed -- waiting for pressure to equalize ... @23:29:48.39 SS.reconfigure :exhale @23:29:48.44 SS.hold @23:30:11.83 PSV.dial :mixloop @23:30:12.32 delay 2 seconds @23:30:14.33 PSV.dial :air @23:30:14.80 delay 2 seconds @23:30:16.82 PSV.dial :mixloop @23:30:17.28 delay 2 seconds @23:30:19.09 Intake.select :close @23:30:19.25 SSV.dial :exhaust @23:30:19.30 PSV.dial :air @23:30:19.78 PS.seek 0ml @23:30:20.01 Exhaust.select :open @23:30:20.13 SS.seek 0ml @23:30:28.99 Exhaust.select :close @23:30:29.10 SSV.dial CBV @23:30:29.15 CBV.dial SSV @23:30:29.90 Allowing 130s for 25.0ml of remaining 625.0ml @23:30:29.91 Intake.select :open @23:30:32.55 Intake @16.5psia, vacuum from 10.0 to 13.0psi @23:30:32.57 SS.reconfigure :inhaling 0.47ml/s @23:30:32.62 SS.seek 25ml @23:30:43.33 washCycleDA #1 of 3 @23:30:43.34 PSR.select :air @23:30:43.36 PSV.dial PSR @23:30:44.70 Pulling 0.5ml of PSR.wash @23:30:44.72 PSR.select :wash @23:30:44.84 PS.reconfigure :default @23:30:44.88 PS.seek 0.5ml @23:30:50.89 delay 5 seconds @23:30:55.91 PSR.select :air @23:30:56.03 PS.seek 2ml @23:31:12.17 PTV.dial :puck @23:31:12.92 PSV.dial PTV @23:31:13.67 PBV.dial :waste1 @23:31:14.09 PS.seek 1.5ml @23:31:17.00 Vacuum too high -- waiting up to 40 seconds @23:31:17.02 SS.reconfigure :exhale @23:31:17.07 SS.hold @23:31:20.11 delay 3 seconds @23:31:23.13 PBV.dial between PSV and waste1 @23:31:23.49 PS.seek 0ml @23:31:29.29 Intake @18.6psia, vacuum from 10.0 to 13.0psi @23:31:29.31 SS.reconfigure :inhaling 0.38ml/s @23:31:29.36 SS.seek 25ml @23:31:39.80 delay 2:00 @23:31:43.16 Stroke completed -- waiting for pressure to equalize ... @23:31:43.17 SS.reconfigure :exhale @23:31:43.22 SS.hold @23:32:03.66 Intake.select :close @23:32:03.82 SSV.dial :exhaust @23:32:04.67 Exhaust.select :open @23:32:04.79 SS.seek 0ml @23:32:13.85 Exhaust.select :close @23:32:13.97 SSV.dial CBV @23:32:13.99 CBV.dial SSV @23:32:14.79 Allowing 130s for 25.0ml of remaining 600.0ml @23:32:14.80 Intake.select :open @23:32:16.97 Intake @16.4psia, vacuum from 10.0 to 13.0psi @23:32:16.99 SS.reconfigure :inhaling 0.38ml/s @23:32:17.04 SS.seek 25ml @23:33:24.69 Stroke completed -- waiting for pressure to equalize ... @23:33:24.70 SS.reconfigure :exhale @23:33:24.75 SS.hold @23:33:39.82 recoverPSRreagent08 @23:33:39.83 PTV.dial :puck @23:33:40.01 PBV.dial :waste1 @23:33:40.81 delay 30 seconds @23:33:53.36 Intake.select :close @23:33:53.52 SSV.dial :exhaust @23:33:54.28 Exhaust.select :open @23:33:54.40 SS.seek 0ml @23:34:03.41 Exhaust.select :close @23:34:03.52 SSV.dial CBV @23:34:03.56 CBV.dial SSV @23:34:04.32 Allowing 130s for 25.0ml of remaining 575.0ml @23:34:04.33 Intake.select :open @23:34:06.50 Intake @16.5psia, vacuum from 10.0 to 13.0psi @23:34:06.52 SS.reconfigure :inhaling 0.38ml/s @23:34:06.57 SS.seek 25ml @23:34:10.87 PBV.dial between PSV and waste1 @23:34:11.23 PSR.select :air @23:34:11.25 PSV.dial PSR @23:34:12.02 PS.reconfigure :fast @23:34:12.07 PS.seek 2.5ml @23:34:36.20 PSV.dial PTV @23:34:36.97 delay 5 seconds @23:34:41.99 PSV.dial PSR @23:34:42.75 delay 5 seconds @23:34:47.77 PSV.dial PTV @23:34:48.55 delay 5 seconds @23:34:53.57 PSV.dial :air @23:34:54.46 PS.seek 0ml @23:35:14.41 Stroke completed -- waiting for pressure to equalize ... @23:35:14.42 SS.reconfigure :exhale @23:35:14.47 SS.hold @23:35:18.09 washCycleDA #2 of 3 @23:35:18.14 PSR.select :air @23:35:18.19 PSV.dial PSR @23:35:19.65 Pulling 0.5ml of PSR.wash @23:35:19.66 PSR.select :wash @23:35:19.78 PS.reconfigure :default @23:35:19.83 PS.seek 0.5ml @23:35:25.78 delay 5 seconds @23:35:30.80 PSR.select :air @23:35:30.99 PS.seek 2ml @23:35:45.30 Intake.select :close @23:35:45.46 SSV.dial :exhaust @23:35:46.22 Exhaust.select :open @23:35:46.34 SS.seek 0ml @23:35:47.13 PTV.dial :puck @23:35:47.21 PSV.dial PTV @23:35:47.96 PBV.dial :waste1 @23:35:48.41 PS.seek 1.5ml @23:35:54.36 delay 3 seconds @23:35:55.83 Exhaust.select :close @23:35:55.94 SSV.dial CBV @23:35:55.97 CBV.dial SSV @23:35:56.75 Allowing 130s for 25.0ml of remaining 550.0ml @23:35:56.77 Intake.select :open @23:35:57.37 PBV.dial between PSV and waste1 @23:35:57.75 PS.seek 0ml @23:35:58.93 Intake @16.5psia, vacuum from 10.0 to 13.0psi @23:35:58.95 SS.reconfigure :inhaling 0.38ml/s @23:35:59.00 SS.seek 25ml @23:36:13.92 delay 2:00 @23:36:59.05 Vacuum too high -- waiting up to 40 seconds @23:36:59.07 SS.reconfigure :exhale @23:36:59.12 SS.hold @23:37:13.64 Intake @18.8psia, vacuum from 10.0 to 13.0psi @23:37:13.66 SS.reconfigure :inhaling 0.31ml/s @23:37:13.72 SS.seek 25ml @23:37:23.81 Stroke completed -- waiting for pressure to equalize ... @23:37:23.82 SS.reconfigure :exhale @23:37:23.87 SS.hold @23:37:44.76 Intake.select :close @23:37:44.99 SSV.dial :exhaust @23:37:45.76 Exhaust.select :open @23:37:45.88 SS.seek 0ml @23:37:54.72 Exhaust.select :close @23:37:54.83 SSV.dial CBV @23:37:54.85 CBV.dial SSV @23:37:55.63 Allowing 130s for 25.0ml of remaining 525.0ml @23:37:55.64 Intake.select :open @23:37:57.81 Intake @16.5psia, vacuum from 10.0 to 13.0psi @23:37:57.83 SS.reconfigure :inhaling 0.31ml/s @23:37:57.88 SS.seek 25ml @23:38:13.94 recoverPSRreagent08 @23:38:13.95 PTV.dial :puck @23:38:14.02 PBV.dial :waste1 @23:38:14.40 delay 30 seconds @23:38:44.43 PBV.dial between PSV and waste1 @23:38:44.80 PSR.select :air @23:38:44.82 PSV.dial PSR @23:38:45.59 PS.reconfigure :fast @23:38:45.64 PS.seek 2.5ml @23:39:09.79 PSV.dial PTV @23:39:10.56 delay 5 seconds @23:39:15.58 PSV.dial PSR @23:39:16.34 delay 5 seconds @23:39:21.36 PSV.dial PTV @23:39:22.12 delay 5 seconds @23:39:24.49 Stroke completed -- waiting for pressure to equalize ... @23:39:24.51 SS.reconfigure :exhale @23:39:24.56 SS.hold @23:39:27.13 PSV.dial :air @23:39:28.06 PS.seek 0ml @23:39:51.35 washCycleDA #3 of 3 @23:39:51.36 PSR.select :air @23:39:51.38 PSV.dial PSR @23:39:52.73 Pulling 0.5ml of PSR.wash @23:39:52.74 PSR.select :wash @23:39:52.86 PS.reconfigure :default @23:39:52.90 PS.seek 0.5ml @23:39:53.26 Intake.select :close @23:39:53.42 SSV.dial :exhaust @23:39:54.19 Exhaust.select :open @23:39:54.31 SS.seek 0ml @23:39:58.91 delay 5 seconds @23:40:03.37 Exhaust.select :close @23:40:03.49 SSV.dial CBV @23:40:03.51 CBV.dial SSV @23:40:03.93 PSR.select :air @23:40:04.04 PS.seek 2ml @23:40:04.31 Allowing 130s for 25.0ml of remaining 500.0ml @23:40:04.32 Intake.select :open @23:40:06.49 Intake @16.5psia, vacuum from 10.0 to 13.0psi @23:40:06.51 SS.reconfigure :inhaling 0.31ml/s @23:40:06.56 SS.seek 25ml @23:40:20.19 PTV.dial :puck @23:40:20.27 PSV.dial PTV @23:40:21.04 PBV.dial :waste1 @23:40:21.95 PS.seek 1.5ml @23:40:27.85 delay 3 seconds @23:40:30.87 PBV.dial between PSV and waste1 @23:40:31.25 PS.seek 0ml @23:40:47.38 delay 2:00 @23:41:32.29 Stroke completed -- waiting for pressure to equalize ... @23:41:32.30 SS.reconfigure :exhale @23:41:32.35 SS.hold @23:42:05.02 Intake.select :close @23:42:05.18 SSV.dial :exhaust @23:42:05.95 Exhaust.select :open @23:42:06.06 SS.seek 0ml @23:42:14.85 Exhaust.select :close @23:42:14.97 SSV.dial CBV @23:42:15.01 CBV.dial SSV @23:42:15.76 Allowing 130s for 25.0ml of remaining 475.0ml @23:42:15.77 Intake.select :open @23:42:17.94 Intake @16.5psia, vacuum from 10.0 to 13.0psi @23:42:17.96 SS.reconfigure :inhaling 0.31ml/s @23:42:18.01 SS.seek 25ml @23:42:47.41 recoverPSRreagent08 @23:42:47.42 PTV.dial :puck @23:42:47.49 PBV.dial :waste1 @23:42:47.86 delay 30 seconds @23:43:17.89 PBV.dial between PSV and waste1 @23:43:18.27 PSR.select :air @23:43:18.29 PSV.dial PSR @23:43:19.05 PS.reconfigure :fast @23:43:19.11 PS.seek 2.5ml @23:43:42.97 PSV.dial PTV @23:43:43.74 delay 5 seconds @23:43:45.10 Stroke completed -- waiting for pressure to equalize ... @23:43:45.11 SS.reconfigure :exhale @23:43:45.16 SS.hold @23:43:48.75 PSV.dial PSR @23:43:49.52 delay 5 seconds @23:43:54.53 PSV.dial PTV @23:43:55.30 delay 5 seconds @23:44:00.31 PSV.dial :air @23:44:01.21 PS.seek 0ml @23:44:24.07 Intake.select :close @23:44:24.23 SSV.dial :exhaust @23:44:24.50 Gate.power :camera,:ON @23:44:24.52 Mixing DA Substrates PSR.select :air @23:44:24.54 PSV.dial PSR @23:44:25.01 Exhaust.select :open @23:44:25.91 SS.seek 0ml @23:44:26.10 Pulling 0.25ml of PSR.sub1 @23:44:26.13 PSR.select :sub1 @23:44:26.38 PS.reconfigure :default @23:44:26.50 PS.seek 0.25ml @23:44:29.97 delay 5 seconds @23:44:34.99 Pulling 0.25ml of PSR.sub2 @23:44:35.01 PSR.select :sub2 @23:44:35.09 Exhaust.select :close @23:44:35.15 PS.seek 0.5ml @23:44:35.21 SSV.dial CBV @23:44:35.25 CBV.dial SSV @23:44:36.01 Allowing 130s for 25.0ml of remaining 450.0ml @23:44:36.02 Intake.select :open @23:44:38.19 Intake @16.5psia, vacuum from 10.0 to 13.0psi @23:44:38.21 SS.reconfigure :inhaling 0.31ml/s @23:44:38.26 SS.seek 25ml @23:44:38.59 delay 5 seconds @23:44:43.61 PSR.select :air @23:44:43.73 PS.seek 2ml @23:44:59.94 PTV.dial :puck @23:45:00.01 PSV.dial PTV @23:45:00.78 PBV.dial :waste1 @23:45:01.23 PS.seek 1.5ml @23:45:07.25 delay 3 seconds @23:45:10.27 PBV.dial between PSV and waste1 @23:45:10.65 PS.seek 0ml @23:45:27.00 delay 10 seconds @23:45:37.03 recoverPSRreagent08 @23:45:37.04 PTV.dial :puck @23:45:37.11 PBV.dial :waste1 @23:45:37.48 delay 30 seconds @23:46:04.17 Vacuum too high -- waiting up to 40 seconds @23:46:04.19 SS.reconfigure :exhale @23:46:04.24 SS.hold @23:46:07.50 PBV.dial between PSV and waste1 @23:46:07.87 PSR.select :air @23:46:07.89 PSV.dial PSR @23:46:08.95 PS.reconfigure :fast @23:46:09.01 PS.seek 2.5ml @23:46:20.73 Intake @17.7psia, vacuum from 10.0 to 13.0psi @23:46:20.75 SS.reconfigure :inhaling 0.25ml/s @23:46:20.80 SS.seek 25ml @23:46:20.94 Stroke completed -- waiting for pressure to equalize ... @23:46:20.95 SS.reconfigure :exhale @23:46:21.00 SS.hold @23:46:33.46 PSV.dial PTV @23:46:34.23 delay 5 seconds @23:46:39.25 PSV.dial PSR @23:46:40.15 delay 5 seconds @23:46:45.17 PSV.dial PTV @23:46:45.97 delay 5 seconds @23:46:46.06 SS.reconfigure :default @23:46:46.12 SS.hold @23:46:46.18 Intake.select :close @23:46:46.34 ShallowSampler::Clogged in WCR -- Filtering too Slowly after 575.0ml @23:46:46.36 SSV.dial :exhaust @23:46:47.14 Exhaust.select :open @23:46:47.26 SS.reconfigure :exhale @23:46:47.31 SS.seek 0ml @23:46:50.99 PSV.dial :air @23:46:51.80 PS.seek 0ml @23:46:56.21 Exhaust.select :close @23:46:56.33 Sampled 575.0ml @23:46:56.34 Drying puck before fixing for 5:00 @23:46:56.35 CBV.dial :waste2b @23:46:57.17 delay 1:00 @23:47:15.84 Additional air clear @23:47:15.85 PSR.select :air @23:47:15.87 PSV.dial PSR @23:47:17.23 Pulling 2ml of PSR.air @23:47:17.24 PSR.select :air @23:47:17.26 PS.reconfigure :default @23:47:17.31 PS.seek 2ml @23:47:38.53 delay 5 seconds @23:47:43.55 PTV.dial :puck @23:47:43.62 PSV.dial PTV @23:47:44.39 PBV.dial :waste1 @23:47:44.82 PS.seek 1.5ml @23:47:50.81 delay 3 seconds @23:47:53.83 PBV.dial between PSV and waste1 @23:47:54.20 delay 1 second @23:47:55.22 PSV.dial :waste1 @23:47:56.36 PS.seek 0ml @23:47:57.20 CSV.dial :air @23:47:57.68 CS.reconfigure :fast @23:47:57.73 CS.seek 10ml @23:48:12.58 Processing Puck Heater at 28C before imaging @23:48:12.59 PH.coast @23:48:12.71 Imaging da puck in Processing Clamp while storing it to tube 5 @23:48:12.89 SC.reconfigure :tight @23:48:12.94 SC.seek 5 @23:48:16.56 SC.configure :limp @23:48:16.78 Elbow.reconfigure :tight @23:48:16.83 Elbow.seek 5 @23:48:18.58 Forearm.reconfigure :tight @23:48:18.63 Forearm.seek 5 + 450 counts @23:48:19.67 SE.reconfigure :pick @23:48:19.73 SE.seek :beamBreak @23:48:22.81 SE.configure :default @23:48:22.90 Pumping wcr puck from tube 6 up to 35psi... @23:48:22.94 CBV.dial SSV @23:48:23.09 Elbow.reconfigure :limp @23:48:23.15 Forearm.reconfigure :loose @23:48:23.19 Forearm.seek 5 @23:48:23.45 Elbow.hold @23:48:23.52 Elbow.reconfigure :tight @23:48:23.63 SE.seek 1.19 Pucks below Top Plate @23:48:24.07 Forearm.force -20 @23:48:24.11 Forearm.seek 5 + 314 counts @23:48:24.27 CBV.dial between CSV and waste2b @23:48:24.47 Forearm.reconfigure :tight @23:48:24.52 Forearm.seek 5 + 450 counts @23:48:24.71 SE.seek 2.19 Pucks below Top Plate @23:48:25.30 Forearm.reconfigure :loose @23:48:25.90 Forearm.seek :retract @23:48:25.92 CSV.dial between air and CTV @23:48:26.31 CS.seek 6.895ml @23:48:26.88 Elbow.seek PC @23:48:29.37 Elbow.reconfigure :loose @23:48:29.47 Forearm.seek PC @23:48:30.40 Hand.close @23:48:31.21 PC.seek :open @23:48:34.36 CTV.dial CSV,avoiding: :air @23:48:34.38 CSV.dial CTV @23:48:35.23 CS.reconfigure :pressurize @23:48:35.28 CS.seek 0ml @23:48:35.98 Forearm.seek :retract @23:48:36.92 Elbow.reconfigure :tight @23:48:36.97 Elbow.seek 5 @23:48:39.56 Forearm.reconfigure :tight @23:48:39.61 Forearm.seek 5 + 450 counts @23:48:40.57 Hand.open @23:48:41.26 SE.seek 1.84 Pucks below Top Plate @23:48:41.56 SE.reconfigure :seal @23:48:41.61 SE.seek 0.88 Pucks below Top Plate @23:48:42.18 Lights, camera, ACTION! @23:48:42.22 PuckCamera: snap -bin=2,2 -auto /var/log/esp/da23dec0723h925ml.tif @23:48:53.41 CSV.dial :air @23:48:53.89 CS.reconfigure :fast @23:48:53.95 CS.seek 10ml @23:49:01.66 Waiting >20 seconds for da to relinquish FlushPuck @23:49:02.15 SX HX9/16 (USB:21): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 16.622 seconds /var/log/esp/da23dec0723h925ml.tif: TIFF Upload Complete @23:49:02.17 PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da23dec0723h925ml15s.tif @23:49:17.79 SX HX9/16 (USB:21): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15 seconds /var/log/esp/midres/da23dec0723h925ml15s.tif: TIFF Upload Complete @23:49:17.81 PuckCamera: snap -bin=2,2 20.0 /var/log/esp/midres/da23dec0723h925ml20s.tif @23:49:19.04 CSV.dial between air and CTV @23:49:19.39 CS.seek 4.39ml @23:49:33.61 CTV.dial CSV,avoiding: :air @23:49:33.63 CSV.dial CTV @23:49:33.99 CS.reconfigure :pressurize @23:49:34.61 CS.seek 0ml @23:49:38.47 SX HX9/16 (USB:21): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 20 seconds /var/log/esp/midres/da23dec0723h925ml20s.tif: TIFF Upload Complete @23:49:38.48 Gate.power :camera,:OFF @23:49:38.50 DAfans.email "16.622 sec auto-exposure",Subject: "da23dec0723h925ml images!" @23:49:38.83 Elbow.seek 5 @23:49:39.23 Forearm.seek 5 + 450 counts @23:49:39.32 SE.reconfigure :gently @23:49:39.38 SE.seek 1.59 Pucks below Top Plate @23:49:40.00 Elbow.reconfigure :limp @23:49:40.06 Forearm.reconfigure :loose @23:49:40.13 Forearm.seek 5 @23:49:40.34 Elbow.hold @23:49:40.43 Elbow.reconfigure :tight @23:49:40.49 SE.reconfigure :default @23:49:40.56 SE.seek 1.78 Pucks below Top Plate @23:49:40.94 Forearm.force -20 @23:49:40.98 Forearm.seek 5 + 315 counts @23:49:41.29 Forearm.reconfigure :tight @23:49:41.34 Forearm.seek 5 + 450 counts @23:49:41.60 SE.seek :down @23:49:42.74 Forearm.reconfigure :loose @23:49:42.80 Forearm.seek :retract @23:49:44.22 SE.stop @23:49:44.44 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @23:49:44.86 Elbow.seek FlushPuck::Garage @23:49:46.47 CSV.dial :air @23:49:46.85 Elbow.reconfigure :loose @23:49:47.06 CS.reconfigure :fast @23:49:47.23 CS.seek 10ml @23:49:48.54 Forearm.seek :garage @23:49:49.57 Hand.close @23:49:50.50 Forearm.seek :retract @23:49:51.56 Elbow.reconfigure :tight @23:49:51.66 Elbow.seek PC @23:49:53.06 Elbow.reconfigure :loose @23:49:53.26 Forearm.reconfigure :tight @23:49:53.36 Forearm.seek PC @23:49:55.08 PC.seek :closed @23:49:59.83 Processing Clamp onSpring/offSpring for 16/26 samples, on/off torque 1.65 @23:49:59.97 Hand.open @23:50:00.73 Forearm.reconfigure :loose @23:50:00.82 Forearm.seek :retract @23:50:01.86 PSR.select :air @23:50:01.92 PSV.dial PSR @23:50:02.44 Pulling 0.5ml of PSR.flush @23:50:02.46 PSR.select :flush @23:50:02.65 PS.seek 0.5ml @23:50:08.61 delay 5 seconds @23:50:11.96 upsync @23:50:12.75 CSV.dial between air and CTV @23:50:13.09 CS.seek 4.489ml @23:50:13.63 PSR.select :air @23:50:13.76 PS.seek 2.5ml @23:50:26.35 CTV.dial CSV,avoiding: :air @23:50:26.37 CSV.dial CTV @23:50:26.73 CS.reconfigure :pressurize @23:50:26.78 CS.seek 0ml @23:50:35.02 delay 1 second @23:50:36.04 PRV.dial :waste2,avoiding: :ab2 @23:50:37.93 PTV.dial :PRVmixing @23:50:38.75 CSV.dial :air @23:50:39.23 CS.reconfigure :fast @23:50:39.28 CS.seek 10ml @23:50:39.71 PSV.dial :mixloop @23:50:40.77 PS.seek :empty @23:51:03.62 CSV.dial between air and CTV @23:51:03.96 CS.seek 4.483ml @23:51:07.15 PSR.select :air @23:51:07.17 PSV.dial PSR @23:51:08.21 Pulling 0.5ml of PSR.flush @23:51:08.22 PSR.select :flush @23:51:08.34 PS.seek 0.5ml @23:51:14.34 delay 5 seconds @23:51:17.29 CTV.dial CSV,avoiding: :air @23:51:17.31 CSV.dial CTV @23:51:17.68 CS.reconfigure :pressurize @23:51:17.73 CS.seek 0ml @23:51:19.35 PSR.select :air @23:51:19.48 PS.seek 2.5ml @23:51:29.72 CSV.dial :air @23:51:30.30 CS.reconfigure :default @23:51:30.35 CS.hold @23:51:30.47 ShallowSampler::Error in MAIN -- Puck.from 6, :wcr reached only 20.3 of 35psi after pumping in 40ml of gas @23:51:30.49 Waiting to Resume from Checkpoint "Pumping wcr puck from tube 6 up to 35psi..." @23:51:30.50 BadNews.email "Puck.from 6, :wcr reached only 20.3 of 35psi after pumping in 40ml of gas",Subject: "ShallowSampler::Error in MAIN" @23:51:35.05 upsync @23:51:40.78 delay 1 second @23:51:41.81 PBV.dial :waste1 @23:51:42.18 PTV.dial :mixing @23:51:42.95 PSV.dial :mixloop @23:51:44.01 PS.seek :empty @23:52:10.34 PSV.dial PSR @23:52:11.38 PSR.select :flush @23:52:11.51 PS.seek 1ml @23:52:22.52 PSR.select :air @23:52:22.64 PS.seek 2.5ml @23:52:38.98 PSV.dial PTV @23:52:39.76 PTV.dial :puck @23:52:40.51 PBV.dial :waste1 @23:52:40.68 PS.seek 0ml @23:53:07.18 PSV.dial PSR @23:53:07.94 PSR.select :flush @23:53:08.07 PS.seek 0.5ml @23:53:13.96 PSR.select :air @23:53:14.08 PS.seek 2.5ml @23:53:35.36 PSV.dial PTV @23:53:36.13 PBV.dial PSV @23:53:36.70 PS.seek 0ml @23:54:03.11 PSV.dial PBV @23:54:03.59 PS.seek 2.5ml @23:54:29.96 PSV.dial :waste1 @23:54:30.71 PS.seek 0ml @23:54:57.02 PSV.dial PSR @23:54:57.49 PSR.select :air @23:54:57.51 PS.seek 2.5ml @23:55:23.83 PSV.dial PTV @23:55:24.69 PBV.dial PSV @23:55:24.77 PS.seek 0ml @23:55:51.14 PSV.dial PBV @23:55:51.60 PS.seek 2.5ml @23:56:17.92 PSV.dial :waste1 @23:56:18.68 PS.seek 0ml @23:56:45.05 PSV.dial PSR @23:56:45.52 PSR.select :flush @23:56:45.64 PS.seek 1ml @23:56:56.67 PSR.select :air @23:56:56.79 PS.seek 2.5ml @23:57:12.95 PSV.dial PTV @23:57:13.81 PTV.dial :puck @23:57:13.83 PBV.dial :waste1 @23:57:14.40 PS.seek 0ml @23:57:40.89 PSV.dial PSR @23:57:41.64 PSR.select :flush @23:57:41.76 PS.seek 0.5ml @23:57:47.67 PSR.select :air @23:57:47.79 PS.seek 2.5ml @23:58:09.11 PSV.dial PTV @23:58:09.87 PBV.dial PSV @23:58:10.44 PS.seek 0ml @23:58:37.21 PSV.dial PBV @23:58:37.74 PS.seek 2.5ml @23:59:04.08 PSV.dial :waste1 @23:59:04.83 PS.seek 0ml @23:59:31.33 PSV.dial PSR @23:59:31.79 PSR.select :air @23:59:31.82 PS.seek 2.5ml @23:59:58.23 PSV.dial PTV @23:59:59.09 PBV.dial PSV @23:59:59.28 PS.seek 0ml @00:00:25.57UTC08-Dec-23 PSV.dial PBV @00:00:26.03 PS.seek 2.5ml @00:00:52.45 PSV.dial :waste1 @00:00:53.21 PS.seek 0ml @00:01:19.61 Garaging FlushPuck in Processing Clamp @00:01:19.68 Elbow.reconfigure :tight @00:01:19.73 Elbow.seek PC @00:01:19.85 Elbow.reconfigure :loose @00:01:19.95 Forearm.seek PC @00:01:20.94 Hand.close @00:01:21.57 PC.seek :open @00:01:26.28 Forearm.seek :retract @00:01:27.25 Elbow.reconfigure :tight @00:01:27.30 Elbow.seek :garage @00:01:28.65 Elbow.reconfigure :loose @00:01:28.75 Forearm.reconfigure :tight @00:01:28.80 Forearm.seek :garage @00:01:29.88 Hand.open @00:01:30.50 Forearm.reconfigure :loose @00:01:30.55 Forearm.seek :retract @01:56:20.66 Session Begun @01:56:28.01 -> Flush FlushPuck is in FlushPuck::Garage @01:56:30.46 -> CC Collection Clamp contains wcr puck from tube 6 @01:56:33.49 -> PC Processing Clamp open @01:57:15.15 -> Puck.count @01:57:15.23 SE.seek :down @01:57:15.32 SE.stop @01:57:15.43 SC.reconfigure :tight @01:57:15.51 SC.seek 1 @01:57:28.54 SC.configure :limp @01:57:29.22 Elbow.reconfigure :tight @01:57:29.30 Elbow.seek 1 @01:57:31.19 Forearm.reconfigure :tight @01:57:31.26 Forearm.seek 1 + 450 counts @01:57:32.33 SE.reconfigure :place @01:57:32.41 SE.seek :beamBreak @01:57:33.58 SE.configure :default @01:57:33.72 SE.seek :down @01:57:34.17 SE.stop @01:57:34.84 Forearm.reconfigure :loose @01:57:34.92 Forearm.seek :retract @01:57:35.90 SC.reconfigure :tight @01:57:35.97 SC.seek 2 @01:57:39.57 SC.configure :limp @01:57:39.74 Elbow.seek 2 @01:57:39.95 Forearm.reconfigure :tight @01:57:40.02 Forearm.seek 2 + 450 counts @01:57:41.06 SE.reconfigure :place @01:57:41.14 SE.seek :beamBreak @01:57:42.28 SE.configure :default @01:57:42.41 SE.seek :down @01:57:42.86 SE.stop @01:57:43.55 Forearm.reconfigure :loose @01:57:43.62 Forearm.seek :retract @01:57:44.59 SC.reconfigure :tight @01:57:44.67 SC.seek 3 @01:57:48.30 SC.configure :limp @01:57:48.48 Elbow.seek 3 @01:57:49.11 Forearm.reconfigure :tight @01:57:49.18 Forearm.seek 3 + 450 counts @01:57:50.21 SE.reconfigure :place @01:57:50.29 SE.seek :beamBreak @01:57:51.41 SE.configure :default @01:57:51.49 Puck::Error in dave -- 33 pucks loaded in tube 3 when expecting 31 @01:57:51.58 SE.seek :down @01:57:52.03 SE.stop @01:57:52.80 Forearm.reconfigure :loose @01:57:52.88 Forearm.seek :retract @01:57:53.86 SC.reconfigure :tight @01:57:53.93 SC.seek 4 @01:57:57.54 SC.configure :limp @01:57:57.72 Elbow.seek 4 @01:57:58.11 Forearm.reconfigure :tight @01:57:58.18 Forearm.seek 4 + 450 counts @01:57:59.22 SE.reconfigure :place @01:57:59.30 SE.seek :beamBreak @01:58:00.44 SE.configure :default @01:58:00.57 SE.seek :down @01:58:01.10 SE.stop @01:58:01.70 Forearm.reconfigure :loose @01:58:01.78 Forearm.seek :retract @01:58:02.77 SC.reconfigure :tight @01:58:02.84 SC.seek 5 @01:58:06.39 SC.configure :limp @01:58:06.58 Elbow.seek 5 @01:58:06.88 Forearm.reconfigure :tight @01:58:06.96 Forearm.seek 5 + 450 counts @01:58:08.00 SE.reconfigure :place @01:58:08.08 SE.seek :beamBreak @01:58:11.22 SE.configure :default @01:58:11.35 SE.seek :down @01:58:12.48 Forearm.reconfigure :loose @01:58:12.55 Forearm.seek :retract @01:58:13.84 SE.stop @01:58:13.90 SC.reconfigure :tight @01:58:13.98 SC.seek 6 @01:58:17.52 SC.configure :limp @01:58:17.69 Elbow.seek 6 @01:58:17.90 Forearm.reconfigure :tight @01:58:17.97 Forearm.seek 6 + 450 counts @01:58:19.02 SE.reconfigure :place @01:58:19.10 SE.seek :beamBreak @01:58:21.02 SE.configure :default @01:58:21.16 SE.seek :down @01:58:21.96 SE.stop @01:58:22.29 Forearm.reconfigure :loose @01:58:22.36 Forearm.seek :retract @01:58:23.38 SC.reconfigure :tight @01:58:23.46 SC.seek 7 @01:58:27.03 SC.configure :limp @01:58:27.21 Elbow.seek 7 @01:58:27.53 Forearm.reconfigure :tight @01:58:27.60 Forearm.seek 7 + 450 counts @01:58:28.64 SE.reconfigure :place @01:58:28.73 SE.seek :beamBreak @01:58:30.04 SE.configure :default @01:58:30.17 SE.seek :down @01:58:30.80 SE.stop @01:58:31.30 Forearm.reconfigure :loose @01:58:31.37 Forearm.seek :retract {1 => 33, 2 => 33, 3 => 33, 4 => 33, 5 => 3, 6 => 29, 7 => 31} @01:59:25.80 -> CC.unloadPuck 5, :WCR @01:59:25.89 Puck::Warning in dave -- Unloading Puck.from 6, :wcr to Storage Carousel @01:59:25.91 Storing WCR puck in Collection Clamp to tube 5 @01:59:26.11 SC.reconfigure :tight @01:59:26.18 SC.seek 5 @01:59:32.91 SC.configure :limp @01:59:33.09 Elbow.seek 5 @01:59:33.66 Forearm.reconfigure :tight @01:59:33.74 Forearm.seek 5 + 450 counts @01:59:34.78 SE.reconfigure :pick @01:59:34.86 SE.seek :beamBreak @01:59:37.90 SE.configure :default @01:59:38.08 Elbow.reconfigure :limp @01:59:38.15 Forearm.reconfigure :loose @01:59:38.22 Forearm.seek 5 @01:59:38.47 Elbow.hold @01:59:38.56 Elbow.reconfigure :tight @01:59:38.67 SE.seek 2.18 Pucks below Top Plate @01:59:39.12 Forearm.force -20 @01:59:39.17 Forearm.seek 5 + 288 counts @01:59:39.96 Forearm.reconfigure :tight @01:59:40.03 Forearm.seek 5 + 450 counts @01:59:40.24 SE.seek 3.18 Pucks below Top Plate @01:59:40.78 Forearm.reconfigure :loose @01:59:40.85 Forearm.seek :retract @01:59:41.80 Elbow.seek CC @01:59:43.54 Elbow.reconfigure :loose @01:59:43.66 Forearm.seek CC @01:59:44.63 Hand.close @01:59:45.23 CC.seek :open @01:59:49.98 Forearm.seek :retract @01:59:51.02 Elbow.reconfigure :tight @01:59:51.10 Elbow.seek 5 @01:59:52.90 Elbow.reconfigure :limp @01:59:52.97 Forearm.seek 5 @01:59:54.02 Elbow.hold @01:59:54.11 Elbow.reconfigure :tight @01:59:54.18 Hand.open @01:59:54.97 Forearm.force -20 @01:59:55.03 Forearm.seek 5 + 319 counts @01:59:55.32 Forearm.reconfigure :tight @01:59:55.39 Forearm.seek 5 + 450 counts @01:59:55.68 SE.seek :down @01:59:56.81 Forearm.reconfigure :loose @01:59:56.88 Forearm.seek :retract @01:59:58.23 SE.stop @02:00:16.85 -> Puck.count @02:00:16.93 SE.seek :down @02:00:17.01 SE.stop @02:00:17.13 SC.reconfigure :tight @02:00:17.21 SC.seek 1 @02:00:30.25 SC.configure :limp @02:00:30.43 Elbow.seek 1 @02:00:30.73 Forearm.reconfigure :tight @02:00:30.80 Forearm.seek 1 + 450 counts @02:00:32.29 SE.reconfigure :place @02:00:32.37 SE.seek :beamBreak @02:00:33.52 SE.configure :default @02:00:33.65 SE.seek :down @02:00:34.11 SE.stop @02:00:34.78 Forearm.reconfigure :loose @02:00:34.85 Forearm.seek :retract @02:00:35.83 SC.reconfigure :tight @02:00:35.90 SC.seek 2 @02:00:39.49 SC.configure :limp @02:00:39.67 Elbow.seek 2 @02:00:39.87 Forearm.reconfigure :tight @02:00:39.95 Forearm.seek 2 + 450 counts @02:00:40.97 SE.reconfigure :place @02:00:41.05 SE.seek :beamBreak @02:00:42.16 SE.configure :default @02:00:42.29 SE.seek :down @02:00:42.73 SE.stop @02:00:43.42 Forearm.reconfigure :loose @02:00:43.49 Forearm.seek :retract @02:00:44.51 SC.reconfigure :tight @02:00:44.58 SC.seek 3 @02:00:48.17 SC.configure :limp @02:00:48.35 Elbow.seek 3 @02:00:48.90 Forearm.reconfigure :tight @02:00:48.97 Forearm.seek 3 + 450 counts @02:00:50.00 SE.reconfigure :place @02:00:50.08 SE.seek :beamBreak @02:00:51.24 SE.configure :default @02:00:51.37 SE.seek :down @02:00:51.81 SE.stop @02:00:52.50 Forearm.reconfigure :loose @02:00:52.57 Forearm.seek :retract @02:00:53.53 SC.reconfigure :tight @02:00:53.60 SC.seek 4 @02:00:57.14 SC.configure :limp @02:00:57.31 Elbow.seek 4 @02:00:57.98 Forearm.reconfigure :tight @02:00:58.06 Forearm.seek 4 + 450 counts @02:00:59.13 SE.reconfigure :place @02:00:59.21 SE.seek :beamBreak @02:01:00.35 SE.configure :default @02:01:00.48 SE.seek :down @02:01:00.95 SE.stop @02:01:01.61 Forearm.reconfigure :loose @02:01:01.68 Forearm.seek :retract @02:01:02.63 SC.reconfigure :tight @02:01:02.71 SC.seek 5 @02:01:06.24 SC.configure :limp @02:01:06.42 Elbow.seek 5 @02:01:06.62 Forearm.reconfigure :tight @02:01:06.70 Forearm.seek 5 + 450 counts @02:01:07.73 SE.reconfigure :place @02:01:07.82 SE.seek :beamBreak @02:01:10.93 SE.configure :default @02:01:11.11 SE.seek :down @02:01:12.24 Forearm.reconfigure :loose @02:01:12.31 Forearm.seek :retract @02:01:13.59 SE.stop @02:01:13.65 SC.reconfigure :tight @02:01:13.72 SC.seek 6 @02:01:17.31 SC.configure :limp @02:01:17.49 Elbow.seek 6 @02:01:17.69 Forearm.reconfigure :tight @02:01:17.76 Forearm.seek 6 + 450 counts @02:01:18.80 SE.reconfigure :place @02:01:18.88 SE.seek :beamBreak @02:01:20.33 SE.configure :default @02:01:20.46 SE.seek :down @02:01:21.20 SE.stop @02:01:21.58 Forearm.reconfigure :loose @02:01:21.66 Forearm.seek :retract @02:01:22.65 SC.reconfigure :tight @02:01:23.12 SC.seek 7 @02:01:26.72 SC.configure :limp @02:01:26.90 Elbow.seek 7 @02:01:27.23 Forearm.reconfigure :tight @02:01:27.31 Forearm.seek 7 + 450 counts @02:01:28.36 SE.reconfigure :place @02:01:28.44 SE.seek :beamBreak @02:01:29.76 SE.configure :default @02:01:29.89 SE.seek :down @02:01:30.50 SE.stop @02:01:31.02 Forearm.reconfigure :loose @02:01:31.10 Forearm.seek :retract {1 => 33, 2 => 33, 3 => 33, 4 => 33, 5 => 4, 6 => 29, 7 => 31} @02:01:57.23 -> intakePurge :intakePurge @02:02:04.07 -> purgeIntake @02:02:04.09 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @02:02:04.29 Elbow.seek FlushPuck::Garage @02:02:06.12 Elbow.reconfigure :loose @02:02:06.24 Forearm.seek :garage @02:02:07.20 Hand.close @02:02:07.96 Forearm.seek :retract @02:02:08.96 Elbow.reconfigure :tight @02:02:09.03 Elbow.seek CC @02:02:12.00 Elbow.reconfigure :loose @02:02:12.11 Forearm.reconfigure :tight @02:02:12.18 Forearm.seek CC @02:02:13.47 CC.seek :closed @02:02:18.01 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.38 @02:02:18.09 Hand.open @02:02:18.78 Forearm.reconfigure :loose @02:02:18.85 Forearm.seek :retract @02:02:20.29 Purging external Intake @02:02:20.43 CSV.dial :flush @02:02:20.91 CS.reconfigure :fast @02:02:20.98 CS.seek 10ml @02:02:45.72 delay 5 seconds @02:02:50.74 CSV.dial CBV @02:02:50.80 CBV.dial CSV @02:02:51.56 CTV.dial :intake @02:02:52.36 Intake.select :open @02:02:52.49 CS.seek 0ml @02:03:16.25 Intake.select :close @02:03:16.42 CTV.dial :air @02:03:17.24 Garaging FlushPuck in Collection Clamp @02:03:17.33 Elbow.reconfigure :tight @02:03:17.40 Elbow.seek CC @02:03:17.67 Elbow.reconfigure :loose @02:03:17.79 Forearm.seek CC @02:03:18.81 Hand.close @02:03:19.47 CC.seek :open @02:03:24.15 Forearm.seek :retract @02:03:25.14 Elbow.reconfigure :tight @02:03:25.22 Elbow.seek :garage @02:03:28.17 Elbow.reconfigure :loose @02:03:28.29 Forearm.reconfigure :tight @02:03:28.36 Forearm.seek :garage @02:03:29.41 Hand.open @02:03:30.15 Forearm.reconfigure :loose @02:03:30.22 Forearm.seek :retract FlushPuck::Garage @02:03:45.60 -> exit @02:03:45.62 Session Ended @13:26:08.25 Session Begun @13:26:19.41 -> Puck.count @13:26:19.50 SE.seek :down @13:26:20.06 SE.stop @13:26:20.18 SC.reconfigure :tight @13:26:20.25 SC.seek 1 @13:26:39.59 SC.configure :limp @13:26:39.83 Elbow.reconfigure :tight @13:26:39.90 Elbow.seek 1 @13:26:41.86 Forearm.reconfigure :tight @13:26:41.93 Forearm.seek 1 + 450 counts @13:26:43.03 SE.reconfigure :place @13:26:43.11 SE.seek :beamBreak @13:26:44.36 SE.configure :default @13:26:44.50 SE.seek :down @13:26:44.97 SE.stop @13:26:45.63 Forearm.reconfigure :loose @13:26:45.70 Forearm.seek :retract @13:26:46.65 SC.reconfigure :tight @13:26:46.72 SC.seek 2 @13:26:50.30 SC.configure :limp @13:26:50.62 Elbow.seek 2 @13:26:50.99 Forearm.reconfigure :tight @13:26:51.07 Forearm.seek 2 + 450 counts @13:26:52.07 SE.reconfigure :place @13:26:52.16 SE.seek :beamBreak @13:26:53.27 SE.configure :default @13:26:53.40 SE.seek :down @13:26:53.86 SE.stop @13:26:54.53 Forearm.reconfigure :loose @13:26:54.60 Forearm.seek :retract @13:26:55.57 SC.reconfigure :tight @13:26:55.65 SC.seek 3 @13:26:59.20 SC.configure :limp @13:26:59.39 Elbow.seek 3 @13:26:59.75 Forearm.reconfigure :tight @13:26:59.83 Forearm.seek 3 + 450 counts @13:27:00.91 SE.reconfigure :place @13:27:00.99 SE.seek :beamBreak @13:27:02.18 SE.configure :default @13:27:02.32 SE.seek :down @13:27:02.76 SE.stop @13:27:03.45 Forearm.reconfigure :loose @13:27:03.52 Forearm.seek :retract @13:27:04.52 SC.reconfigure :tight @13:27:04.59 SC.seek 4 @13:27:08.16 SC.configure :limp @13:27:08.43 Elbow.seek 4 @13:27:08.95 Forearm.reconfigure :tight @13:27:09.03 Forearm.seek 4 + 450 counts @13:27:10.20 SE.reconfigure :place @13:27:10.28 SE.seek :beamBreak @13:27:11.41 SE.configure :default @13:27:11.55 SE.seek :down @13:27:12.03 SE.stop @13:27:12.67 Forearm.reconfigure :loose @13:27:12.74 Forearm.seek :retract @13:27:13.70 SC.reconfigure :tight @13:27:13.77 SC.seek 5 @13:27:17.30 SC.configure :limp @13:27:17.47 Elbow.seek 5 @13:27:17.82 Forearm.reconfigure :tight @13:27:17.89 Forearm.seek 5 + 450 counts @13:27:18.96 SE.reconfigure :place @13:27:19.04 SE.seek :beamBreak @13:27:22.16 SE.configure :default @13:27:22.29 SE.seek :down @13:27:23.42 Forearm.reconfigure :loose @13:27:23.49 Forearm.seek :retract @13:27:24.71 SE.stop @13:27:24.77 SC.reconfigure :tight @13:27:24.84 SC.seek 6 @13:27:28.39 SC.configure :limp @13:27:28.56 Elbow.seek 6 @13:27:28.76 Forearm.reconfigure :tight @13:27:29.30 Forearm.seek 6 + 450 counts @13:27:30.35 SE.reconfigure :place @13:27:30.44 SE.seek :beamBreak @13:27:31.91 SE.configure :default @13:27:32.04 SE.seek :down @13:27:32.78 SE.stop @13:27:33.17 Forearm.reconfigure :loose @13:27:33.26 Forearm.seek :retract @13:27:34.25 SC.reconfigure :tight @13:27:34.33 SC.seek 7 @13:27:37.87 SC.configure :limp @13:27:38.05 Elbow.seek 7 @13:27:38.37 Forearm.reconfigure :tight @13:27:38.44 Forearm.seek 7 + 450 counts @13:27:39.49 SE.reconfigure :place @13:27:39.58 SE.seek :beamBreak @13:27:40.89 SE.configure :default @13:27:41.02 SE.seek :down @13:27:41.68 SE.stop @13:27:42.16 Forearm.reconfigure :loose @13:27:42.23 Forearm.seek :retract {1 => 33, 2 => 33, 3 => 33, 4 => 33, 5 => 4, 6 => 29, 7 => 31} @13:28:07.80 -> abort! 'mission complete' @13:28:07.81 CmdServer::Client::Error in dave -- Aborting Mission: mission complete # @13:28:07.87 BadNews.email "mission complete",Subject: "DA_WCR_120723b ABORTED!" @13:28:12.52 upsync @13:28:12.61 Session Ended + ESPwhich DA_WCR_120823.rb + fn=DA_WCR_120823.rb + IFS=: + set /home/esp/esp2/mission . + fullPath=/home/esp/esp2/mission/DA_WCR_120823.rb + [ -f /home/esp/esp2/mission/DA_WCR_120823.rb ] + realpath /home/esp/esp2/mission/DA_WCR_120823.rb + return 0 + scriptPath=/home/esp/esp2local/mission/DA_WCR_120823.rb + exec ruby /home/esp/esp2/lib/esp.rb /home/esp/esp2local/mission/DA_WCR_120823.rb @13:34:31.49UTC08-Dec-23
ESP2 v3.42 I2C Gateway v3.20 for core bus @13:34:34.18 Configuration for short puck jake 1/11/21 by brent@mbari.org /home/esp/esp2/type/shallow/jake/configure.rb @13:34:37.84 Polling Can every 10:00 @13:34:37.96 Cleanly exited at 13:31:41UTC08-Dec-23 @13:34:38.01 Loading /home/esp/esp2local/mission/DA_WCR_120823.rb mission @13:34:38.07 Preparing to sleep until + 00:00:05 @13:34:38.09 GoodNews.email "Starting DA_WCR_120823 w/pucks in tube 6\n{1 => 33,\n 2 => 33,\n 3 => 33,\n 4 => 33,\n 5 => 4,\n 6 => 29,\n 7 => 31}\nCTD data unavailable\nCan@13:34:37, 31% flow, 16.2C, 32% humidity, 15.1psia, 13.705V, 0.327A, 177.672Ah, 4.48W",Subject: "DA_WCR_120823 begun" @13:34:38.15
Asleep til + 00:00:05 @13:34:38.16 Continuing at 13:34 @13:34:38.45 Gate.power :core,:OFF @13:34:38.46 Gate.power :raw,:OFF @13:34:38.47 Shutdown @13:34:41.24 GoodNews.email "Then, in tube 6: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_120823.rb:14\n{1 => 33,\n 2 => 33,\n 3 => 31,\n 4 => 33,\n 5 => 4,\n 6 => 29,\n 7 => 31}\nCTD data unavailable\nCan@13:34:38, 31% flow, 16.2C, 32% humidity, 15.1psia, 13.700V, 0.315A, 177.672Ah, 4.31W",Subject: "Asleep until 13:34" @13:34:42.62 upsync @13:34:43.05
Powering core bus @13:34:45.60 upsync @13:34:48.71
Gate.power :raw,:ON @13:34:49.48 CS.configure :default @13:34:49.53 PS.configure :default @13:34:49.58 SS.configure :default @13:34:49.62 CC.configure :default @13:34:49.67 PC.configure :default @13:34:49.74 Forearm.configure :loose @13:34:49.79 Elbow.configure :loose @13:34:49.85 SC.configure :loose @13:34:49.90 SE.configure :default @13:34:49.95 CH.configure :default @13:34:49.98 PH.configure :default @13:34:50.02 All dwarf controllers running firmware version 3.85 @13:34:50.13 Valve.reset @13:34:50.39 Intake.select :close @13:34:50.45 Exhaust.select :close @13:34:50.46 CTV.dial :air @13:34:50.55 PSV.dial :air @13:34:50.58 CSV.dial :waste1 @13:34:50.72
Forearm.home.seek :retract @13:34:51.10 SSV.dial :spit,avoiding: :exhaust @13:34:51.43 Hand.close @13:34:51.45
Elbow.reconfigure :loose @13:34:51.55 Elbow.jog 1500 @13:34:51.82 PS.home.seek 0ml @13:34:51.96 CS.reconfigure :fast @13:34:51.99 SS.home.seek 0ml @13:34:52.09 CS.home.seek 0ml @13:34:52.17
Elbow.reconfigure :loose @13:34:52.22 Elbow.home.seek :clear @13:34:55.55 SE.home.seek :down @13:34:56.17 SC.reconfigure :tight @13:34:56.23 SC.home.seek :initial @13:35:00.30 PS.stop @13:35:24.57
SC.configure :limp @13:35:24.63 Forearm.seek :retract @13:35:24.69 Elbow.seek :hammer @13:35:25.74 Forearm.reconfigure :tight @13:35:25.79 Forearm.seek retracted @13:35:26.36 CC.home.seek :open @13:35:26.44 PC.home.seek :open @13:35:29.86
Phase [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] @13:35:29.88 Notice.email "At 13:35:29 began in tube 6: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_120823.rb:14\n{1 => 33,\n 2 => 33,\n 3 => 31,\n 4 => 33,\n 5 => 4,\n 6 => 29,\n 7 => 31}\nCTD data unavailable\nCan@13:35:29, 31% flow, 16.2C, 32% humidity, 15.1psia, 13.700V, 0.309A, 177.677Ah, 4.23W",Subject: "Started DA_WCR_120823 phase initialPurge; da [1000, 1000] #da and wsr are run simultanelously" @13:35:29.89
Starting initialPurge @13:35:29.92 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @13:35:29.98 Hand.open @13:35:30.73 Elbow.reconfigure :tight @13:35:30.78 Elbow.seek FlushPuck::Garage @13:35:32.69 Elbow.reconfigure :loose @13:35:33.06 Forearm.reconfigure :loose @13:35:33.15 Forearm.seek :garage @13:35:34.13 Hand.close @13:35:34.38 upsync @13:35:34.87
Forearm.seek :retract @13:35:35.84 Elbow.reconfigure :tight @13:35:35.89 Elbow.seek CC @13:35:38.86 Elbow.reconfigure :loose @13:35:38.97 Forearm.reconfigure :tight @13:35:39.01 Forearm.seek CC @13:35:40.23 CC.seek :closed @13:35:44.76 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.38 @13:35:44.83 Hand.open @13:35:45.55 Forearm.reconfigure :loose @13:35:45.60 Forearm.seek :retract @13:35:46.55 Flushing the Collection Valve::Manifold @13:35:46.68 CSV.dial CSR @13:35:47.97 Pulling 5ml of CSR.flush @13:35:47.98 CSR.select :flush @13:35:48.09 CS.reconfigure :default @13:35:48.14 CS.seek 5ml @13:36:02.41 delay 5 seconds @13:36:07.43 CSR.select :air @13:36:07.60 CS.reconfigure :fast @13:36:07.64 CS.seek 7.499ml @13:36:14.53 CSV.dial :waste @13:36:15.83 CS.reconfigure :default @13:36:15.88 CS.seek 0ml @13:36:35.91 Purging external Intake @13:36:36.03 CSV.dial :flush @13:36:36.49 CS.reconfigure :fast @13:36:36.54 CS.seek 10ml @13:37:01.00 delay 5 seconds @13:37:06.02 CSV.dial CBV @13:37:06.06
CBV.dial CSV @13:37:06.81 CTV.dial :intake @13:37:07.61 Intake.select :open @13:37:07.72 CS.seek 0ml @13:37:31.37 Intake.select :close @13:37:31.48 CTV.dial :air @13:37:32.32 Flushing upper manifold @13:37:32.49 CSV.dial :flush @13:37:33.24 CS.seek 5ml @13:37:45.97 delay 5 seconds @13:37:50.99 CSV.dial :air @13:37:51.60 CS.seek 7ml @13:37:57.16 CSV.dial CBV @13:37:57.18
CBV.dial CSV @13:37:58.20 CTV.dial :air @13:37:58.29 CS.seek 0ml @13:38:14.97 delay 7 seconds @13:38:22.00 CS.seek 3ml @13:38:30.09 CSV.dial :waste @13:38:30.55 CS.seek 0ml @13:38:38.36 CSV.dial :flush @13:38:38.82 CS.seek 3ml @13:38:46.87 delay 5 seconds @13:38:51.89 CSV.dial :air @13:38:52.39 CS.seek 5ml @13:38:57.97 CSV.dial :air @13:38:58.05 CS.seek 0ml @13:39:10.37 delay 7 seconds @13:39:17.40 CS.seek 3ml @13:39:25.43 CSV.dial :waste @13:39:26.18 CS.seek 0ml @13:39:33.94 Garaging FlushPuck in Collection Clamp @13:39:34.01 Elbow.reconfigure :tight @13:39:34.06 Elbow.seek CC @13:39:34.40 Elbow.reconfigure :loose @13:39:34.55 Forearm.seek CC @13:39:35.50 Hand.close @13:39:36.11 CC.seek :open @13:39:41.17 Forearm.seek :retract @13:39:42.21 Elbow.reconfigure :tight @13:39:42.25 Elbow.seek :garage @13:39:45.32 Elbow.reconfigure :loose @13:39:45.42 Forearm.reconfigure :tight @13:39:45.47 Forearm.seek :garage @13:39:46.53 Hand.open @13:39:47.17 Forearm.reconfigure :loose @13:39:47.22 Forearm.seek :retract @13:39:48.18 da sampling at most 1000ml, wcr at most 1000ml @13:39:48.21 Loading sh1 puck from tube 6 into Collection Clamp @13:39:48.23 DAfans.email "Started da in tube 6 at 13:39:48UTC08-Dec-23\n{1 => 33,\n 2 => 33,\n 3 => 31,\n 4 => 33,\n 5 => 4,\n 6 => 29,\n 7 => 31}\nCTD data unavailable\nCan@13:35:29, 31% flow, 16.2C, 32% humidity, 15.1psia, 13.700V, 0.309A, 177.677Ah, 4.23W",Subject: "da sampling at most 1000ml, wcr at most 1000ml" @13:39:48.49
SC.reconfigure :tight @13:39:48.57 SC.seek 6 @13:39:52.63 upsync @13:39:55.28
SC.configure :limp @13:39:55.51 Elbow.reconfigure :tight @13:39:55.56 Elbow.seek 6 @13:39:57.54 Forearm.reconfigure :tight @13:39:57.59 Forearm.seek 6 + 450 counts @13:39:58.68 SE.reconfigure :pick @13:39:58.74 SE.seek :beamBreak @13:40:00.00 SE.configure :default @13:40:00.17 Elbow.seek 6 @13:40:00.36 Forearm.seek 6 + 300 counts @13:40:00.58 Forearm.reconfigure :loose @13:40:00.63 Hand.close @13:40:01.25 SE.seek :down @13:40:01.46
Forearm.seek :retract @13:40:02.12 SE.stop @13:40:02.82
Elbow.seek CC @13:40:04.42 Elbow.reconfigure :loose @13:40:04.52 Forearm.reconfigure :tight @13:40:04.57 Forearm.seek CC @13:40:05.87 CC.seek :closed @13:40:10.38 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.26 @13:40:10.39 Hand.open @13:40:11.04 Forearm.reconfigure :loose @13:40:11.09 Forearm.seek :retract @13:40:12.03 collectSample 1000ml w/bubble point of 24psi @13:40:12.05 Priming 7ml @13:40:12.06 Intake.select :close @13:40:12.07 Exhaust.select :close @13:40:12.08 SS.hold @13:40:12.28 Calibrating pressure and temperature sensors @13:40:12.31 CTV.dial :air @13:40:12.32 SSV.dial :spit,avoiding: :exhaust @13:40:12.41 SS.reconfigure :fast @13:40:12.45 SS.seek 5ml @13:40:15.50 SSV.dial CBV @13:40:15.54
CBV.dial SSV @13:40:16.85 delay 3.8 seconds @13:40:20.79 Sensor limits: Intake max 100.3psia; Outlet max 93.6psia @13:40:20.80 SSV.dial :spit @13:40:22.11 SS.seek 0ml @13:40:24.74 SSV.dial CBV @13:40:26.06 CTV.dial :intake @13:40:26.84 SSV.dial CBV @13:40:26.87
CBV.dial SSV @13:40:26.95 Allowing 40s for 7.0ml of remaining 7.0ml @13:40:26.96 Intake.select :open @13:40:29.13 Intake @14.6psia, vacuum from 9.1 to 12.1psi @13:40:29.14 SS.reconfigure :inhaling 2.20ml/s @13:40:29.19 SS.seek 7ml @13:40:35.90 Stroke completed -- waiting for pressure to equalize ... @13:40:35.91 SS.reconfigure :exhale @13:40:35.95 SS.hold @13:40:44.95 Intake.select :close @13:40:45.10 SSV.dial :exhaust @13:40:45.85 Exhaust.select :open @13:40:45.97 SS.seek 0ml @13:40:50.21 Exhaust.select :close @13:40:50.32 Sampling 1000.0ml @13:40:50.33 Intake.select :close @13:40:50.34 Exhaust.select :close @13:40:50.36 SS.reconfigure :default @13:40:50.40 SS.hold @13:40:50.61 CTV.dial :intake @13:40:50.62 SSV.dial CBV @13:40:50.64
CBV.dial SSV @13:40:51.42 Allowing 130s for 25.0ml of remaining 1000.0ml @13:40:51.43 Intake.select :open @13:40:53.59 Intake @15.5psia, vacuum from 10.0 to 13.0psi @13:40:53.61 SS.reconfigure :inhaling 2.20ml/s @13:40:53.66 SS.seek 25ml @13:41:02.13 Vacuum too high -- waiting up to 40 seconds @13:41:02.14 SS.reconfigure :exhale @13:41:02.19 SS.hold @13:41:09.48 Intake @19.1psia, vacuum from 10.0 to 13.0psi @13:41:09.50 SS.reconfigure :inhaling 1.76ml/s @13:41:09.54 SS.seek 25ml @13:41:12.09 Vacuum too high -- waiting up to 40 seconds @13:41:12.11 SS.reconfigure :exhale @13:41:12.16 SS.hold @13:41:13.68 Intake @18.4psia, vacuum from 10.0 to 13.0psi @13:41:13.69 SS.reconfigure :inhaling 1.41ml/s @13:41:13.74 SS.seek 25ml @13:41:16.38 Vacuum too high -- waiting up to 40 seconds @13:41:16.39 SS.reconfigure :exhale @13:41:16.44 SS.hold @13:41:18.62 Intake @18.8psia, vacuum from 10.0 to 13.0psi @13:41:18.64 SS.reconfigure :inhaling 1.13ml/s @13:41:18.68 SS.seek 25ml @13:41:21.69 Vacuum too high -- waiting up to 40 seconds @13:41:21.70 SS.reconfigure :exhale @13:41:21.75 SS.hold @13:41:23.45 Intake @18.7psia, vacuum from 10.0 to 13.0psi @13:41:23.47 SS.reconfigure :inhaling 0.91ml/s @13:41:23.51 SS.seek 25ml @13:41:27.69 Vacuum too high -- waiting up to 40 seconds @13:41:27.70 SS.reconfigure :exhale @13:41:27.75 SS.hold @13:41:28.75 Intake @18.6psia, vacuum from 10.0 to 13.0psi @13:41:28.77 SS.reconfigure :inhaling 0.73ml/s @13:41:28.82 SS.seek 25ml @13:41:29.29 Stroke completed -- waiting for pressure to equalize ... @13:41:29.30 SS.reconfigure :exhale @13:41:29.34 SS.hold @13:41:52.26 Intake.select :close @13:41:52.41 SSV.dial :exhaust @13:41:53.18 Exhaust.select :open @13:41:53.29 SS.seek 0ml @13:42:02.12 Exhaust.select :close @13:42:02.22 SSV.dial CBV @13:42:02.26
CBV.dial SSV @13:42:03.01 Allowing 130s for 25.0ml of remaining 975.0ml @13:42:03.02 Intake.select :open @13:42:05.18 Intake @15.9psia, vacuum from 10.0 to 13.0psi @13:42:05.19 SS.reconfigure :inhaling 0.73ml/s @13:42:05.24 SS.seek 25ml @13:42:41.61 Stroke completed -- waiting for pressure to equalize ... @13:42:41.62 SS.reconfigure :exhale @13:42:41.67 SS.hold @13:43:08.65 Intake.select :close @13:43:08.80 SSV.dial :exhaust @13:43:09.57 Exhaust.select :open @13:43:09.68 SS.seek 0ml @13:43:18.75 Exhaust.select :close @13:43:18.86 SSV.dial CBV @13:43:18.88
CBV.dial SSV @13:43:19.66 Allowing 130s for 25.0ml of remaining 950.0ml @13:43:19.67 Intake.select :open @13:43:21.84 Intake @15.9psia, vacuum from 10.0 to 13.0psi @13:43:21.85 SS.reconfigure :inhaling 0.73ml/s @13:43:21.90 SS.seek 25ml @13:43:57.80 Stroke completed -- waiting for pressure to equalize ... @13:43:57.81 SS.reconfigure :exhale @13:43:57.90 SS.hold @13:44:20.65 Intake.select :close @13:44:20.80 SSV.dial :exhaust @13:44:21.57 Exhaust.select :open @13:44:21.68 SS.seek 0ml @13:44:30.52 Exhaust.select :close @13:44:30.63 SSV.dial CBV @13:44:30.65
CBV.dial SSV @13:44:31.43 Allowing 130s for 25.0ml of remaining 925.0ml @13:44:31.44 Intake.select :open @13:44:33.60 Intake @16.1psia, vacuum from 10.0 to 13.0psi @13:44:33.61 SS.reconfigure :inhaling 0.73ml/s @13:44:33.66 SS.seek 25ml @13:45:10.09 Stroke completed -- waiting for pressure to equalize ... @13:45:10.10 SS.reconfigure :exhale @13:45:10.15 SS.hold @13:45:29.21 Intake.select :close @13:45:29.37 SSV.dial :exhaust @13:45:30.13 Exhaust.select :open @13:45:30.24 SS.seek 0ml @13:45:38.99 Exhaust.select :close @13:45:39.09 SSV.dial CBV @13:45:39.13
CBV.dial SSV @13:45:39.88 Allowing 130s for 25.0ml of remaining 900.0ml @13:45:39.89 Intake.select :open @13:45:42.05 Intake @16.1psia, vacuum from 10.0 to 13.0psi @13:45:42.07 SS.reconfigure :inhaling 0.73ml/s @13:45:42.12 SS.seek 25ml @13:46:18.59 Stroke completed -- waiting for pressure to equalize ... @13:46:18.60 SS.reconfigure :exhale @13:46:18.65 SS.hold @13:46:37.40 Intake.select :close @13:46:37.56 SSV.dial :exhaust @13:46:38.31 Exhaust.select :open @13:46:38.43 SS.seek 0ml @13:46:47.14 Exhaust.select :close @13:46:47.25 SSV.dial CBV @13:46:47.27
CBV.dial SSV @13:46:48.05 Allowing 130s for 25.0ml of remaining 875.0ml @13:46:48.06 Intake.select :open @13:46:50.23 Intake @16.1psia, vacuum from 10.0 to 13.0psi @13:46:50.24 SS.reconfigure :inhaling 0.73ml/s @13:46:50.29 SS.seek 25ml @13:47:26.53 Stroke completed -- waiting for pressure to equalize ... @13:47:26.54 SS.reconfigure :exhale @13:47:26.59 SS.hold @13:47:43.24 Intake.select :close @13:47:43.39 SSV.dial :exhaust @13:47:44.16 Exhaust.select :open @13:47:44.27 SS.seek 0ml @13:47:53.01 Exhaust.select :close @13:47:53.12 SSV.dial CBV @13:47:53.14
CBV.dial SSV @13:47:53.92 Allowing 130s for 25.0ml of remaining 850.0ml @13:47:53.93 Intake.select :open @13:47:56.10 Intake @16.1psia, vacuum from 10.0 to 13.0psi @13:47:56.11 SS.reconfigure :inhaling 0.73ml/s @13:47:56.16 SS.seek 25ml @13:48:32.57 Stroke completed -- waiting for pressure to equalize ... @13:48:32.58 SS.reconfigure :exhale @13:48:32.63 SS.hold @13:48:47.00 Intake.select :close @13:48:47.15 SSV.dial :exhaust @13:48:47.94 Exhaust.select :open @13:48:48.05 SS.seek 0ml @13:48:56.72 Exhaust.select :close @13:48:56.82 SSV.dial CBV @13:48:56.84
CBV.dial SSV @13:48:57.62 Allowing 130s for 25.0ml of remaining 825.0ml @13:48:57.64 Intake.select :open @13:48:59.80 Intake @16.1psia, vacuum from 10.0 to 13.0psi @13:48:59.82 SS.reconfigure :inhaling 0.73ml/s @13:48:59.87 SS.seek 25ml @13:49:36.06 Stroke completed -- waiting for pressure to equalize ... @13:49:36.07 SS.reconfigure :exhale @13:49:36.47 SS.hold @13:49:49.02 Intake.select :close @13:49:49.17 SSV.dial :exhaust @13:49:49.95 Exhaust.select :open @13:49:50.07 SS.seek 0ml @13:49:58.79 Exhaust.select :close @13:49:58.91 SSV.dial CBV @13:49:58.95
CBV.dial SSV @13:49:59.69 Allowing 130s for 25.0ml of remaining 800.0ml @13:49:59.70 Intake.select :open @13:50:01.86 Intake @16.2psia, vacuum from 10.0 to 13.0psi @13:50:01.88 SS.reconfigure :inhaling 0.73ml/s @13:50:01.93 SS.seek 25ml @13:50:38.15 Stroke completed -- waiting for pressure to equalize ... @13:50:38.16 SS.reconfigure :exhale @13:50:38.21 SS.hold @13:50:51.37 Intake.select :close @13:50:51.52 SSV.dial :exhaust @13:50:52.28 Exhaust.select :open @13:50:52.39 SS.seek 0ml @13:51:01.12 Exhaust.select :close @13:51:01.23 SSV.dial CBV @13:51:01.25
CBV.dial SSV @13:51:02.03 Allowing 130s for 25.0ml of remaining 775.0ml @13:51:02.04 Intake.select :open @13:51:04.20 Intake @16.0psia, vacuum from 10.0 to 13.0psi @13:51:04.22 SS.reconfigure :inhaling 0.73ml/s @13:51:04.27 SS.seek 25ml @13:51:40.51 Stroke completed -- waiting for pressure to equalize ... @13:51:40.52 SS.reconfigure :exhale @13:51:40.56 SS.hold @13:51:55.26 Intake.select :close @13:51:55.42 SSV.dial :exhaust @13:51:56.18 Exhaust.select :open @13:51:56.30 SS.seek 0ml @13:52:05.03 Exhaust.select :close @13:52:05.14 SSV.dial CBV @13:52:05.17
CBV.dial SSV @13:52:05.92 Allowing 130s for 25.0ml of remaining 750.0ml @13:52:05.93 Intake.select :open @13:52:08.10 Intake @16.1psia, vacuum from 10.0 to 13.0psi @13:52:08.11 SS.reconfigure :inhaling 0.73ml/s @13:52:08.16 SS.seek 25ml @13:52:44.34 Stroke completed -- waiting for pressure to equalize ... @13:52:44.35 SS.reconfigure :exhale @13:52:44.39 SS.hold @13:52:57.03 Intake.select :close @13:52:57.19 SSV.dial :exhaust @13:52:57.95 Exhaust.select :open @13:52:58.06 SS.seek 0ml @13:53:06.98 Exhaust.select :close @13:53:07.08 SSV.dial CBV @13:53:07.10
CBV.dial SSV @13:53:07.87 Allowing 130s for 25.0ml of remaining 725.0ml @13:53:07.88 Intake.select :open @13:53:10.47 Intake @16.0psia, vacuum from 10.0 to 13.0psi @13:53:10.48 SS.reconfigure :inhaling 0.73ml/s @13:53:10.53 SS.seek 25ml @13:53:46.29 Stroke completed -- waiting for pressure to equalize ... @13:53:46.30 SS.reconfigure :exhale @13:53:46.34 SS.hold @13:54:00.65 Intake.select :close @13:54:00.80 SSV.dial :exhaust @13:54:01.57 Exhaust.select :open @13:54:01.68 SS.seek 0ml @13:54:10.66 Exhaust.select :close @13:54:10.78 SSV.dial CBV @13:54:10.81
CBV.dial SSV @13:54:11.56 Allowing 130s for 25.0ml of remaining 700.0ml @13:54:11.57 Intake.select :open @13:54:13.73 Intake @16.1psia, vacuum from 10.0 to 13.0psi @13:54:13.74 SS.reconfigure :inhaling 0.73ml/s @13:54:13.79 SS.seek 25ml @13:54:49.74 Stroke completed -- waiting for pressure to equalize ... @13:54:49.75 SS.reconfigure :exhale @13:54:49.80 SS.hold @13:55:04.38 Intake.select :close @13:55:04.54 SSV.dial :exhaust @13:55:05.32 Exhaust.select :open @13:55:05.43 SS.seek 0ml @13:55:14.22 Exhaust.select :close @13:55:14.33 SSV.dial CBV @13:55:14.36
CBV.dial SSV @13:55:15.13 Allowing 130s for 25.0ml of remaining 675.0ml @13:55:15.14 Intake.select :open @13:55:17.31 Intake @16.1psia, vacuum from 10.0 to 13.0psi @13:55:17.32 SS.reconfigure :inhaling 0.73ml/s @13:55:17.37 SS.seek 25ml @13:55:53.63 Stroke completed -- waiting for pressure to equalize ... @13:55:53.64 SS.reconfigure :exhale @13:55:53.69 SS.hold @13:56:08.02 Intake.select :close @13:56:08.18 SSV.dial :exhaust @13:56:08.94 Exhaust.select :open @13:56:09.06 SS.seek 0ml @13:56:17.79 Exhaust.select :close @13:56:17.90 SSV.dial CBV @13:56:17.93
CBV.dial SSV @13:56:18.68 Allowing 130s for 25.0ml of remaining 650.0ml @13:56:18.69 Intake.select :open @13:56:20.86 Intake @16.2psia, vacuum from 10.0 to 13.0psi @13:56:20.87 SS.reconfigure :inhaling 0.73ml/s @13:56:20.92 SS.seek 25ml @13:56:57.16 Stroke completed -- waiting for pressure to equalize ... @13:56:57.17 SS.reconfigure :exhale @13:56:57.22 SS.hold @13:57:11.55 Intake.select :close @13:57:11.71 SSV.dial :exhaust @13:57:12.47 Exhaust.select :open @13:57:12.59 SS.seek 0ml @13:57:21.50 Exhaust.select :close @13:57:21.61 SSV.dial CBV @13:57:21.63
CBV.dial SSV @13:57:22.41 Allowing 130s for 25.0ml of remaining 625.0ml @13:57:22.42 Intake.select :open @13:57:24.59 Intake @16.2psia, vacuum from 10.0 to 13.0psi @13:57:24.61 SS.reconfigure :inhaling 0.73ml/s @13:57:24.66 SS.seek 25ml @13:58:00.44 Stroke completed -- waiting for pressure to equalize ... @13:58:00.45 SS.reconfigure :exhale @13:58:00.50 SS.hold @13:58:16.68 Intake.select :close @13:58:16.84 SSV.dial :exhaust @13:58:17.60 Exhaust.select :open @13:58:17.71 SS.seek 0ml @13:58:26.47 Exhaust.select :close @13:58:26.58 SSV.dial CBV @13:58:26.60
CBV.dial SSV @13:58:27.38 Allowing 130s for 25.0ml of remaining 600.0ml @13:58:27.39 Intake.select :open @13:58:29.91 Intake @16.1psia, vacuum from 10.0 to 13.0psi @13:58:30.00 SS.reconfigure :inhaling 0.73ml/s @13:58:30.04 SS.seek 25ml @13:59:06.39 Stroke completed -- waiting for pressure to equalize ... @13:59:06.40 SS.reconfigure :exhale @13:59:06.45 SS.hold @13:59:22.65 Intake.select :close @13:59:22.80 SSV.dial :exhaust @13:59:23.56 Exhaust.select :open @13:59:23.68 SS.seek 0ml @13:59:32.39 Exhaust.select :close @13:59:32.50 SSV.dial CBV @13:59:32.52
CBV.dial SSV @13:59:33.30 Allowing 130s for 25.0ml of remaining 575.0ml @13:59:33.31 Intake.select :open @13:59:35.48 Intake @16.1psia, vacuum from 10.0 to 13.0psi @13:59:35.49 SS.reconfigure :inhaling 0.73ml/s @13:59:35.54 SS.seek 25ml @14:00:11.84 Stroke completed -- waiting for pressure to equalize ... @14:00:11.85 SS.reconfigure :exhale @14:00:11.90 SS.hold @14:00:30.44 Intake.select :close @14:00:30.59 SSV.dial :exhaust @14:00:31.36 Exhaust.select :open @14:00:31.47 SS.seek 0ml @14:00:40.39 Exhaust.select :close @14:00:40.50 SSV.dial CBV @14:00:40.52
CBV.dial SSV @14:00:41.30 Allowing 130s for 25.0ml of remaining 550.0ml @14:00:41.31 Intake.select :open @14:00:43.48 Intake @16.1psia, vacuum from 10.0 to 13.0psi @14:00:43.49 SS.reconfigure :inhaling 0.73ml/s @14:00:43.54 SS.seek 25ml @14:01:19.40 Stroke completed -- waiting for pressure to equalize ... @14:01:19.41 SS.reconfigure :exhale @14:01:19.46 SS.hold @14:01:37.82 Intake.select :close @14:01:37.98 SSV.dial :exhaust @14:01:38.73 Exhaust.select :open @14:01:38.85 SS.seek 0ml @14:01:47.75 Exhaust.select :close @14:01:47.86 SSV.dial CBV @14:01:47.88
CBV.dial SSV @14:01:48.66 Allowing 130s for 25.0ml of remaining 525.0ml @14:01:48.67 Intake.select :open @14:01:50.83 Intake @16.0psia, vacuum from 10.0 to 13.0psi @14:01:50.84 SS.reconfigure :inhaling 0.73ml/s @14:01:50.89 SS.seek 25ml @14:02:27.12 Stroke completed -- waiting for pressure to equalize ... @14:02:27.13 SS.reconfigure :exhale @14:02:27.18 SS.hold @14:02:45.51 Intake.select :close @14:02:45.66 SSV.dial :exhaust @14:02:46.42 Exhaust.select :open @14:02:46.54 SS.seek 0ml @14:02:55.25 Exhaust.select :close @14:02:55.35 SSV.dial CBV @14:02:55.38
CBV.dial SSV @14:02:56.14 Allowing 130s for 25.0ml of remaining 500.0ml @14:02:56.15 Intake.select :open @14:02:58.32 Intake @16.2psia, vacuum from 10.0 to 13.0psi @14:02:58.33 SS.reconfigure :inhaling 0.73ml/s @14:02:58.38 SS.seek 25ml @14:03:34.67 Stroke completed -- waiting for pressure to equalize ... @14:03:34.68 SS.reconfigure :exhale @14:03:34.73 SS.hold @14:03:53.40 Intake.select :close @14:03:53.56 SSV.dial :exhaust @14:03:54.32 Exhaust.select :open @14:03:54.44 SS.seek 0ml @14:04:03.40 Exhaust.select :close @14:04:03.51 SSV.dial CBV @14:04:03.54
CBV.dial SSV @14:04:04.31 Allowing 130s for 25.0ml of remaining 475.0ml @14:04:04.32 Intake.select :open @14:04:06.49 Intake @16.2psia, vacuum from 10.0 to 13.0psi @14:04:06.50 SS.reconfigure :inhaling 0.73ml/s @14:04:06.55 SS.seek 25ml @14:04:42.40 Stroke completed -- waiting for pressure to equalize ... @14:04:42.41 SS.reconfigure :exhale @14:04:42.46 SS.hold @14:05:03.18 Intake.select :close @14:05:03.33 SSV.dial :exhaust @14:05:04.10 Exhaust.select :open @14:05:04.22 SS.seek 0ml @14:05:13.15 Exhaust.select :close @14:05:13.26 SSV.dial CBV @14:05:13.28
CBV.dial SSV @14:05:14.06 Allowing 130s for 25.0ml of remaining 450.0ml @14:05:14.07 Intake.select :open @14:05:16.24 Intake @16.2psia, vacuum from 10.0 to 13.0psi @14:05:16.25 SS.reconfigure :inhaling 0.73ml/s @14:05:16.30 SS.seek 25ml @14:05:52.09 Stroke completed -- waiting for pressure to equalize ... @14:05:52.10 SS.reconfigure :exhale @14:05:52.14 SS.hold @14:06:14.64 Intake.select :close @14:06:14.80 SSV.dial :exhaust @14:06:15.56 Exhaust.select :open @14:06:15.67 SS.seek 0ml @14:06:24.57 Exhaust.select :close @14:06:24.68 SSV.dial CBV @14:06:24.70
CBV.dial SSV @14:06:25.48 Allowing 130s for 25.0ml of remaining 425.0ml @14:06:25.49 Intake.select :open @14:06:27.66 Intake @16.2psia, vacuum from 10.0 to 13.0psi @14:06:27.67 SS.reconfigure :inhaling 0.73ml/s @14:06:27.72 SS.seek 25ml @14:07:03.46 Stroke completed -- waiting for pressure to equalize ... @14:07:03.47 SS.reconfigure :exhale @14:07:03.52 SS.hold @14:07:26.24 Intake.select :close @14:07:26.47 SSV.dial :exhaust @14:07:27.24 Exhaust.select :open @14:07:27.36 SS.seek 0ml @14:07:36.31 Exhaust.select :close @14:07:36.42 SSV.dial CBV @14:07:36.44
CBV.dial SSV @14:07:37.21 Allowing 130s for 25.0ml of remaining 400.0ml @14:07:37.22 Intake.select :open @14:07:39.40 Intake @16.3psia, vacuum from 10.0 to 13.0psi @14:07:39.41 SS.reconfigure :inhaling 0.73ml/s @14:07:39.46 SS.seek 25ml @14:08:15.32 Stroke completed -- waiting for pressure to equalize ... @14:08:15.33 SS.reconfigure :exhale @14:08:15.37 SS.hold @14:08:39.77 Intake.select :close @14:08:40.34 SSV.dial :exhaust @14:08:41.10 Exhaust.select :open @14:08:41.22 SS.seek 0ml @14:08:49.99 Exhaust.select :close @14:08:50.10 SSV.dial CBV @14:08:50.12
CBV.dial SSV @14:08:50.90 Allowing 130s for 25.0ml of remaining 375.0ml @14:08:50.91 Intake.select :open @14:08:53.08 Intake @16.2psia, vacuum from 10.0 to 13.0psi @14:08:53.10 SS.reconfigure :inhaling 0.73ml/s @14:08:53.14 SS.seek 25ml @14:09:26.34 Vacuum too high -- waiting up to 40 seconds @14:09:26.35 SS.reconfigure :exhale @14:09:26.41 SS.hold @14:09:37.64 Intake @17.4psia, vacuum from 10.0 to 13.0psi @14:09:37.65 SS.reconfigure :inhaling 0.59ml/s @14:09:37.70 SS.seek 25ml @14:09:41.71 Stroke completed -- waiting for pressure to equalize ... @14:09:41.72 SS.reconfigure :exhale @14:09:41.76 SS.hold @14:09:56.47 Intake.select :close @14:09:56.62 SSV.dial :exhaust @14:09:57.38 Exhaust.select :open @14:09:57.49 SS.seek 0ml @14:10:06.41 Exhaust.select :close @14:10:06.52 SSV.dial CBV @14:10:06.54
CBV.dial SSV @14:10:07.32 Allowing 130s for 25.0ml of remaining 350.0ml @14:10:07.33 Intake.select :open @14:10:09.50 Intake @16.2psia, vacuum from 10.0 to 13.0psi @14:10:09.51 SS.reconfigure :inhaling 0.59ml/s @14:10:09.56 SS.seek 25ml @14:10:53.94 Stroke completed -- waiting for pressure to equalize ... @14:10:53.95 SS.reconfigure :exhale @14:10:54.00 SS.hold @14:11:16.37 Intake.select :close @14:11:16.52 SSV.dial :exhaust @14:11:17.30 Exhaust.select :open @14:11:17.41 SS.seek 0ml @14:11:26.36 Exhaust.select :close @14:11:26.47 SSV.dial CBV @14:11:26.49
CBV.dial SSV @14:11:27.27 Allowing 130s for 25.0ml of remaining 325.0ml @14:11:27.28 Intake.select :open @14:11:29.45 Intake @16.3psia, vacuum from 10.0 to 13.0psi @14:11:29.47 SS.reconfigure :inhaling 0.59ml/s @14:11:29.51 SS.seek 25ml @14:12:13.77 Stroke completed -- waiting for pressure to equalize ... @14:12:13.78 SS.reconfigure :exhale @14:12:13.82 SS.hold @14:12:38.20 Intake.select :close @14:12:38.36 SSV.dial :exhaust @14:12:39.11 Exhaust.select :open @14:12:39.23 SS.seek 0ml @14:12:48.00 Exhaust.select :close @14:12:48.11 SSV.dial CBV @14:12:48.13
CBV.dial SSV @14:12:48.91 Allowing 130s for 25.0ml of remaining 300.0ml @14:12:48.92 Intake.select :open @14:12:51.09 Intake @16.2psia, vacuum from 10.0 to 13.0psi @14:12:51.10 SS.reconfigure :inhaling 0.59ml/s @14:12:51.15 SS.seek 25ml @14:13:35.94 Stroke completed -- waiting for pressure to equalize ... @14:13:35.95 SS.reconfigure :exhale @14:13:35.99 SS.hold @14:14:03.10 Intake.select :close @14:14:03.25 SSV.dial :exhaust @14:14:04.02 Exhaust.select :open @14:14:04.13 SS.seek 0ml @14:14:12.92 Exhaust.select :close @14:14:13.03 SSV.dial CBV @14:14:13.05
CBV.dial SSV @14:14:13.83 Allowing 130s for 25.0ml of remaining 275.0ml @14:14:13.84 Intake.select :open @14:14:16.01 Intake @16.2psia, vacuum from 10.0 to 13.0psi @14:14:16.03 SS.reconfigure :inhaling 0.59ml/s @14:14:16.08 SS.seek 25ml @14:14:55.46 Vacuum too high -- waiting up to 40 seconds @14:14:55.47 SS.reconfigure :exhale @14:14:55.52 SS.hold @14:15:07.17 Intake @18.2psia, vacuum from 10.0 to 13.0psi @14:15:07.19 SS.reconfigure :inhaling 0.47ml/s @14:15:07.24 SS.seek 25ml @14:15:14.75 Stroke completed -- waiting for pressure to equalize ... @14:15:14.76 SS.reconfigure :exhale @14:15:14.80 SS.hold @14:15:31.02 Intake.select :close @14:15:31.18 SSV.dial :exhaust @14:15:31.95 Exhaust.select :open @14:15:32.07 SS.seek 0ml @14:15:40.89 Exhaust.select :close @14:15:41.00 SSV.dial CBV @14:15:41.02
CBV.dial SSV @14:15:41.85 Allowing 130s for 25.0ml of remaining 250.0ml @14:15:41.86 Intake.select :open @14:15:44.02 Intake @16.2psia, vacuum from 10.0 to 13.0psi @14:15:44.04 SS.reconfigure :inhaling 0.47ml/s @14:15:44.51 SS.seek 25ml @14:16:39.87 Stroke completed -- waiting for pressure to equalize ... @14:16:39.88 SS.reconfigure :exhale @14:16:39.93 SS.hold @14:17:02.40 Intake.select :close @14:17:02.55 SSV.dial :exhaust @14:17:03.31 Exhaust.select :open @14:17:03.42 SS.seek 0ml @14:17:12.29 Exhaust.select :close @14:17:12.40 SSV.dial CBV @14:17:12.42
CBV.dial SSV @14:17:13.20 Allowing 130s for 25.0ml of remaining 225.0ml @14:17:13.21 Intake.select :open @14:17:15.38 Intake @16.4psia, vacuum from 10.0 to 13.0psi @14:17:15.39 SS.reconfigure :inhaling 0.47ml/s @14:17:15.44 SS.seek 25ml @14:18:10.67 Stroke completed -- waiting for pressure to equalize ... @14:18:10.68 SS.reconfigure :exhale @14:18:10.72 SS.hold @14:18:35.60 Intake.select :close @14:18:35.76 SSV.dial :exhaust @14:18:36.51 Exhaust.select :open @14:18:36.62 SS.seek 0ml @14:18:45.62 Exhaust.select :close @14:18:45.73 SSV.dial CBV @14:18:45.75
CBV.dial SSV @14:18:46.53 Allowing 130s for 25.0ml of remaining 200.0ml @14:18:46.54 Intake.select :open @14:18:48.70 Intake @16.4psia, vacuum from 10.0 to 13.0psi @14:18:48.72 SS.reconfigure :inhaling 0.47ml/s @14:18:48.77 SS.seek 25ml @14:19:43.71 Stroke completed -- waiting for pressure to equalize ... @14:19:43.72 SS.reconfigure :exhale @14:19:43.77 SS.hold @14:20:12.52 Intake.select :close @14:20:12.67 SSV.dial :exhaust @14:20:13.45 Exhaust.select :open @14:20:13.56 SS.seek 0ml @14:20:22.57 Exhaust.select :close @14:20:22.68 SSV.dial CBV @14:20:22.70
CBV.dial SSV @14:20:23.48 Allowing 130s for 25.0ml of remaining 175.0ml @14:20:23.49 Intake.select :open @14:20:25.66 Intake @16.4psia, vacuum from 10.0 to 13.0psi @14:20:25.67 SS.reconfigure :inhaling 0.47ml/s @14:20:25.72 SS.seek 25ml @14:21:20.83 Stroke completed -- waiting for pressure to equalize ... @14:21:20.84 SS.reconfigure :exhale @14:21:21.24 SS.hold @14:21:49.71 Intake.select :close @14:21:49.87 SSV.dial :exhaust @14:21:50.63 Exhaust.select :open @14:21:50.75 SS.seek 0ml @14:21:59.76 Exhaust.select :close @14:21:59.86 SSV.dial CBV @14:21:59.88
CBV.dial SSV @14:22:00.71 Allowing 130s for 25.0ml of remaining 150.0ml @14:22:00.72 Intake.select :open @14:22:02.89 Intake @16.4psia, vacuum from 10.0 to 13.0psi @14:22:02.90 SS.reconfigure :inhaling 0.47ml/s @14:22:02.95 SS.seek 25ml @14:22:58.36 Stroke completed -- waiting for pressure to equalize ... @14:22:58.37 SS.reconfigure :exhale @14:22:58.42 SS.hold @14:23:31.12 Intake.select :close @14:23:31.27 SSV.dial :exhaust @14:23:32.05 Exhaust.select :open @14:23:32.17 SS.seek 0ml @14:23:41.16 Exhaust.select :close @14:23:41.69 SSV.dial CBV @14:23:41.73
CBV.dial SSV @14:23:42.48 Allowing 130s for 25.0ml of remaining 125.0ml @14:23:42.49 Intake.select :open @14:23:44.65 Intake @16.3psia, vacuum from 10.0 to 13.0psi @14:23:44.66 SS.reconfigure :inhaling 0.47ml/s @14:23:44.71 SS.seek 25ml @14:24:39.83 Stroke completed -- waiting for pressure to equalize ... @14:24:39.84 SS.reconfigure :exhale @14:24:39.89 SS.hold @14:25:15.03 Intake.select :close @14:25:15.18 SSV.dial :exhaust @14:25:15.94 Exhaust.select :open @14:25:16.06 SS.seek 0ml @14:25:25.11 Exhaust.select :close @14:25:25.23 SSV.dial CBV @14:25:25.26
CBV.dial SSV @14:25:26.02 Allowing 130s for 25.0ml of remaining 100.0ml @14:25:26.04 Intake.select :open @14:25:28.20 Intake @16.3psia, vacuum from 10.0 to 13.0psi @14:25:28.22 SS.reconfigure :inhaling 0.47ml/s @14:25:28.27 SS.seek 25ml @14:26:21.85 Vacuum too high -- waiting up to 40 seconds @14:26:21.87 SS.reconfigure :exhale @14:26:21.92 SS.hold @14:26:38.36 Intake @18.2psia, vacuum from 10.0 to 13.0psi @14:26:38.37 SS.reconfigure :inhaling 0.38ml/s @14:26:38.42 SS.seek 25ml @14:26:40.58 Stroke completed -- waiting for pressure to equalize ... @14:26:40.59 SS.reconfigure :exhale @14:26:40.63 SS.hold @14:27:03.34 Intake.select :close @14:27:03.49 SSV.dial :exhaust @14:27:04.26 Exhaust.select :open @14:27:04.38 SS.seek 0ml @14:27:13.36 Exhaust.select :close @14:27:13.47 SSV.dial CBV @14:27:13.49
CBV.dial SSV @14:27:14.27 Allowing 130s for 25.0ml of remaining 75.0ml @14:27:14.28 Intake.select :open @14:27:16.44 Intake @16.4psia, vacuum from 10.0 to 13.0psi @14:27:16.46 SS.reconfigure :inhaling 0.38ml/s @14:27:16.51 SS.seek 25ml @14:28:24.26 Stroke completed -- waiting for pressure to equalize ... @14:28:24.27 SS.reconfigure :exhale @14:28:24.32 SS.hold @14:28:59.34 Intake.select :close @14:28:59.49 SSV.dial :exhaust @14:29:00.27 Exhaust.select :open @14:29:00.38 SS.seek 0ml @14:29:09.42 Exhaust.select :close @14:29:09.53 SSV.dial CBV @14:29:09.55
CBV.dial SSV @14:29:10.33 Allowing 130s for 25.0ml of remaining 50.0ml @14:29:10.34 Intake.select :open @14:29:12.50 Intake @16.3psia, vacuum from 10.0 to 13.0psi @14:29:12.52 SS.reconfigure :inhaling 0.38ml/s @14:29:12.57 SS.seek 25ml @14:30:20.96 Stroke completed -- waiting for pressure to equalize ... @14:30:20.97 SS.reconfigure :exhale @14:30:21.01 SS.hold @14:30:55.79 Intake.select :close @14:30:55.94 SSV.dial :exhaust @14:30:56.70 Exhaust.select :open @14:30:56.81 SS.seek 0ml @14:31:06.04 Exhaust.select :close @14:31:06.14 SSV.dial CBV @14:31:06.16
CBV.dial SSV @14:31:06.94 Allowing 130s for 25.0ml of remaining 25.0ml @14:31:06.95 Intake.select :open @14:31:09.11 Intake @16.4psia, vacuum from 10.0 to 13.0psi @14:31:09.12 SS.reconfigure :inhaling 0.38ml/s @14:31:09.18 SS.seek 24.99ml @14:32:17.84 Stroke completed -- waiting for pressure to equalize ... @14:32:17.85 SS.reconfigure :exhale @14:32:17.89 SS.hold @14:32:52.50 Intake.select :close @14:32:52.66 SSV.dial :exhaust @14:32:53.46 Exhaust.select :open @14:32:53.58 SS.seek 0ml @14:33:02.38 Exhaust.select :close @14:33:02.49 Sampled 1000.0ml @14:33:02.51 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @14:33:02.52 Intake.select :close @14:33:02.58 CSV.dial :waste @14:33:02.65 CS.seek 0ml @14:33:02.74 CSV.dial :air @14:33:02.77 SSV.dial CBV,avoiding: :exhaust @14:33:02.79
CBV.dial SSV @14:33:03.53 CS.seek 10ml @14:33:04.70
CTV.dial :air @14:33:05.48 delay 15 seconds @14:33:20.50 SSV.dial :spit,avoiding: :exhaust @14:33:21.83 CTV.dial CSV,avoiding: :intake @14:33:21.86
SS.seek 0ml @14:33:21.98 SSV.dial CBV,avoiding: :exhaust @14:33:28.20 SSV.dial between CBV and plug @14:33:28.67 CBV.dial between SSV and waste2 @14:33:29.05 CSV.dial between air and CTV @14:33:29.38 CS.seek 8.281ml @14:33:34.20 CTV.dial CSV,avoiding: :air @14:33:34.22
CSV.dial CTV @14:33:34.59 CS.reconfigure :pressurize @14:33:34.64 CS.seek 0ml @14:33:48.79 BubblePoint: Intake @24.2psia, Outlet @1.0psia @14:33:48.80 CBV.dial SSV @14:33:49.16 SS.reconfigure :default @14:33:49.20 SS.seek 10ml @14:33:54.78 delay 15 seconds @14:34:09.90 Evacuated: Intake @23.7psia, Outlet @23.3psia @14:34:09.92 SSV.dial :spit,avoiding: :exhaust @14:34:10.97 SS.reconfigure :exhale @14:34:11.02 SS.seek 0ml @14:34:15.97 SS.reconfigure :fast @14:34:16.02 SS.seek 5ml @14:34:18.81 SSV.dial CBV,avoiding: :exhaust @14:34:18.83
CBV.dial SSV @14:34:20.20 CTV.dial :air,avoiding: :intake @14:34:21.54 CSV.dial CTV @14:34:21.58
SS.seek 10ml @14:34:23.20 CSV.dial :air @14:34:23.64 CS.reconfigure :fast @14:34:23.69 CS.seek 0ml @14:34:24.50
SSV.dial :spit,avoiding: :exhaust @14:34:25.83 SS.reconfigure :exhale @14:34:25.88 SS.seek 0ml @14:34:30.88 SS.reconfigure :fast @14:34:30.93 SS.seek 5ml @14:34:31.23 CS.stop @14:34:33.84 SSV.dial CBV,avoiding: :exhaust @14:34:33.86
CBV.dial SSV @14:34:36.22 SSV.dial :spit,avoiding: :exhaust @14:34:37.53 SS.reconfigure :default @14:34:37.58 SS.seek 0ml @14:34:40.76 SS.stop @14:34:40.80 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @14:34:40.81 Intake.select :close @14:34:40.87 CSV.dial :waste @14:34:41.00 SSV.dial CBV,avoiding: :exhaust @14:34:41.02
CBV.dial SSV @14:34:41.65 CS.seek 0ml @14:34:41.73 CSV.dial :air @14:34:42.35
CTV.dial :air @14:34:42.47 CS.seek 10ml @14:34:42.53
delay 15 seconds @14:34:57.56 SSV.dial :spit,avoiding: :exhaust @14:34:58.89 CTV.dial CSV,avoiding: :intake @14:34:58.92
SS.reconfigure :exhale @14:34:59.02 SS.seek 0ml @14:34:59.40 SSV.dial CBV,avoiding: :exhaust @14:35:06.92 SSV.dial between CBV and plug @14:35:07.41 CBV.dial between SSV and waste2 @14:35:07.82 CSV.dial between air and CTV @14:35:08.14 CS.seek 7.513ml @14:35:14.61 CTV.dial CSV,avoiding: :air @14:35:14.63
CSV.dial CTV @14:35:14.98 CS.reconfigure :pressurize @14:35:15.03 CS.seek 0ml @14:35:28.85 BubblePoint: Intake @24.1psia, Outlet @3.4psia @14:35:28.86 CBV.dial SSV @14:35:29.18 SS.reconfigure :default @14:35:29.23 SS.seek 10ml @14:35:34.80 delay 15 seconds @14:35:49.93 Evacuated: Intake @23.8psia, Outlet @23.7psia @14:35:50.30 SSV.dial :spit,avoiding: :exhaust @14:35:51.40 SS.reconfigure :exhale @14:35:51.45 SS.seek 0ml @14:35:56.39 SS.reconfigure :fast @14:35:56.44 SS.seek 5ml @14:35:59.40 SSV.dial CBV,avoiding: :exhaust @14:35:59.43
CBV.dial SSV @14:36:00.75 CTV.dial :air,avoiding: :intake @14:36:02.07 CSV.dial CTV @14:36:02.11
SS.seek 10ml @14:36:03.31 CSV.dial :air @14:36:03.76 CS.reconfigure :fast @14:36:03.81 CS.seek 0ml @14:36:04.74
SSV.dial :spit,avoiding: :exhaust @14:36:06.08 SS.reconfigure :exhale @14:36:06.12 SS.seek 0ml @14:36:10.66 CS.stop @14:36:11.14
SS.reconfigure :fast @14:36:11.19 SS.seek 5ml @14:36:14.10 SSV.dial CBV,avoiding: :exhaust @14:36:14.13
CBV.dial SSV @14:36:16.45 SSV.dial :spit,avoiding: :exhaust @14:36:17.76 SS.reconfigure :default @14:36:17.81 SS.seek 0ml @14:36:21.00 SS.stop @14:36:21.05 Lysing: Adding 2.0ml AqMeOH @14:36:21.06 CBV.dial between CSV and waste1,avoiding: :DBV @14:36:22.55 CTV.dial CSV @14:36:23.88 CSV.dial CSR @14:36:24.79 Pulling 1ml of CSR.AqMeOH @14:36:24.80 CSR.select :AqMeOH @14:36:24.92 CS.reconfigure :default @14:36:24.96 CS.seek 1ml @14:36:28.41 delay 5 seconds @14:36:33.43 CSR.select :air @14:36:33.60 CS.seek 6.001ml @14:36:47.23 delay 2 seconds @14:36:49.24 CSV.dial :waste2 @14:36:49.72 CS.reconfigure :fast @14:36:49.77 CS.seek 0ml @14:37:04.22 CSV.dial CSR @14:37:04.68 Pulling 2ml of CSR.AqMeOH @14:37:04.69 CSR.select :AqMeOH @14:37:04.80 CS.reconfigure :default @14:37:04.85 CS.seek 2ml @14:37:11.01 delay 5 seconds @14:37:16.03 CSR.select :air @14:37:16.19 CS.seek 5ml @14:37:24.88 delay 2 seconds @14:37:26.90 CSV.dial CTV @14:37:27.36 CS.seek 1ml @14:37:38.58 Before lysing Collection Puck Heater at 12.4C/30.8psia @14:37:38.60 Heating Puck.from 6, :sh1 in Collection Clamp to 70C for 10:00 @14:37:38.62 CH.seek 70C,8:00 @14:39:53.33 CH.hold 10:00,70C @14:39:53.53 Begun lysing Collection Puck Heater at 68C/37.6psia @14:49:53.54 Ended lysing Collection Puck Heater at 70C/37.0psia @14:49:53.56 CS.seek 0ml @14:49:56.94 CTV.dial between spare and air,avoiding: :intake @14:49:57.96 CSV.dial :air @14:49:58.41 CS.seek 5ml @14:50:12.21 CSV.dial CTV @14:50:12.67 delay 2 seconds @14:50:14.68 CSV.dial :air @14:50:15.14 delay 2 seconds @14:50:17.15 CSV.dial CTV @14:50:17.61 delay 2 seconds @14:50:19.63 CSV.dial :air @14:50:20.07 delay 2 seconds @14:50:22.10 CS.seek 0ml @14:50:35.73 Puck @79.2psia after final lysis pressurization @14:50:35.75 Recovering Lysate into Collection syringe @14:50:35.76 CSV.dial CBV @14:50:36.78 CBV.dial CSV @14:50:37.16 CS.seek 5ml @14:50:50.93 delay 5 seconds @14:50:55.95 CTV.dial :air @14:50:56.47 CS.seek 7ml @14:51:02.37 delay 5 seconds @14:51:07.39 CSV.dial :air2 @14:51:07.83 CS.seek 10ml @14:51:16.53 CSV.dial between air and CTV,avoiding: CBV @14:51:17.69 Lysing Complete @14:51:17.75 Storing sh1 puck in Collection Clamp to tube 5 @14:51:17.93 SC.reconfigure :tight @14:51:17.98 SC.seek 5 @14:51:21.56 SC.configure :limp @14:51:22.21 Elbow.reconfigure :tight @14:51:22.26 Elbow.seek 5 @14:51:24.07 Forearm.reconfigure :tight @14:51:24.12 Forearm.seek 5 + 450 counts @14:51:25.26 SE.reconfigure :pick @14:51:25.32 SE.seek :beamBreak @14:51:28.24 SE.configure :default @14:51:28.40 Elbow.reconfigure :limp @14:51:28.45 Forearm.reconfigure :loose @14:51:28.49 Forearm.seek 5 @14:51:28.73 Elbow.hold @14:51:28.79 Elbow.reconfigure :tight @14:51:28.89 SE.seek 3.18 Pucks below Top Plate @14:51:29.32 Forearm.force -20 @14:51:29.36 Forearm.seek 5 + 286 counts @14:51:29.66 Forearm.reconfigure :tight @14:51:29.71 Forearm.seek 5 + 450 counts @14:51:29.90 SE.seek 4.18 Pucks below Top Plate @14:51:30.42 Forearm.reconfigure :loose @14:51:30.47 Forearm.seek :retract @14:51:31.39 Elbow.seek CC @14:51:33.10 Elbow.reconfigure :loose @14:51:33.20 Forearm.seek CC @14:51:34.15 Hand.close @14:51:34.81 CC.seek :open @14:51:39.53 Forearm.seek :retract @14:51:40.51 Elbow.reconfigure :tight @14:51:40.56 Elbow.seek 5 @14:51:42.31 Elbow.reconfigure :limp @14:51:42.73 Forearm.seek 5 @14:51:43.76 Elbow.hold @14:51:43.82 Elbow.reconfigure :tight @14:51:43.87 Hand.open @14:51:44.56 Forearm.force -20 @14:51:44.60 Forearm.seek 5 + 314 counts @14:51:44.91 Forearm.reconfigure :tight @14:51:44.96 Forearm.seek 5 + 450 counts @14:51:45.23 SE.seek :down @14:51:46.36
Forearm.reconfigure :loose @14:51:46.40 Forearm.seek :retract @14:51:47.66 SE.stop @14:51:47.72
Tranferring DA @14:51:47.74 Beginning samplePassC @14:51:47.77 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:51:48.04 Elbow.seek FlushPuck::Garage @14:51:49.89 Elbow.reconfigure :loose @14:51:49.99 Forearm.seek :garage @14:51:51.01 Hand.close @14:51:51.80 Forearm.seek :retract @14:51:52.75 Elbow.reconfigure :tight @14:51:52.79 Elbow.seek CC @14:51:55.74 Elbow.reconfigure :loose @14:51:55.84 Forearm.reconfigure :tight @14:51:55.89 Forearm.seek CC @14:51:57.18 CC.seek :closed @14:52:01.71 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.47 @14:52:01.78 Hand.open @14:52:02.49 Forearm.reconfigure :loose @14:52:02.54 Forearm.seek :retract @14:52:03.54 PSV.dial PSR @14:52:04.93 Pulling 1.5ml of PSR.flush @14:52:04.94 PSR.select :flush @14:52:05.06 PS.seek 1.5ml @14:52:21.25 delay 5 seconds @14:52:26.27 PSV.dial :waste1 @14:52:26.75 PS.seek 0ml @14:52:42.93 PSV.dial PSR @14:52:43.40 PSR.select :air @14:52:43.51 PS.seek 2.5ml @14:53:09.88 PRV.dial CBV,avoiding: :ab2 @14:53:09.90
CBV.dial PRV @14:53:11.65 PSV.dial :mixloop @14:53:12.70 CTV.dial :air,avoiding: :intake @14:53:12.88 PTV.dial :PRVmixing @14:53:14.22 PS.seek 0ml @14:53:40.55 PSV.dial :air @14:53:41.03 PS.seek 2.5ml @14:54:07.37 PSV.dial :mixloop @14:54:07.84 PS.seek 0ml @14:54:34.37 PSV.dial :air @14:54:34.84 PS.seek 2.5ml @14:55:01.21 PSV.dial :mixloop @14:55:01.69 PS.seek 0ml @14:55:28.09 Garaging FlushPuck in Collection Clamp @14:55:28.16 Elbow.reconfigure :tight @14:55:28.21 Elbow.seek CC @14:55:28.45 Elbow.reconfigure :loose @14:55:28.55 Forearm.seek CC @14:55:29.52 Hand.close @14:55:30.18 CC.seek :open @14:55:35.26 Forearm.seek :retract @14:55:36.23 Elbow.reconfigure :tight @14:55:36.27 Elbow.seek :garage @14:55:39.22 Elbow.reconfigure :loose @14:55:39.32 Forearm.reconfigure :tight @14:55:39.37 Forearm.seek :garage @14:55:40.45 Hand.open @14:55:41.11 Forearm.reconfigure :loose @14:55:41.16 Forearm.seek :retract @14:55:42.18 PSV.dial :mixloop @14:55:42.26 PTV.dial :PRVmixing @14:55:42.34 PRV.dial CBV @14:55:42.36
CBV.dial PRV @14:55:42.60 CTV.dial :air,avoiding: :intake @14:55:42.62 Loading lyfil puck from tube 6 into Collection Clamp @14:55:42.85 SC.reconfigure :tight @14:55:42.89 SC.seek 6 @14:55:46.52 SC.configure :limp @14:55:46.73 Elbow.reconfigure :tight @14:55:46.78 Elbow.seek 6 @14:55:48.70 Forearm.reconfigure :tight @14:55:48.75 Forearm.seek 6 + 450 counts @14:55:49.83 SE.reconfigure :pick @14:55:49.89 SE.seek :beamBreak @14:55:51.17 SE.configure :default @14:55:51.33 Elbow.seek 6 @14:55:51.51 Forearm.seek 6 + 300 counts @14:55:51.74 Forearm.reconfigure :loose @14:55:51.79 Hand.close @14:55:52.82 SE.seek :down @14:55:53.02
Forearm.seek :retract @14:55:53.76 SE.stop @14:55:53.99
Elbow.seek CC @14:55:55.63 Elbow.reconfigure :loose @14:55:55.73 Forearm.reconfigure :tight @14:55:55.78 Forearm.seek CC @14:55:57.07 CC.seek :closed @14:56:01.60 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.13 @14:56:01.61 Hand.open @14:56:02.24 Forearm.reconfigure :loose @14:56:02.28 Forearm.seek :retract @14:56:03.29 CTV.dial CSV,avoiding: :intake @14:56:04.63 CSV.dial CTV @14:56:05.07 PS.seek 2.5ml @14:56:31.55 CS.seek 4.5ml @14:56:46.46 delay 8 seconds @14:56:54.48 PSV.dial :air @14:56:54.94 delay 2 seconds @14:56:56.96 PSV.dial :waste2 @14:56:57.82 PS.seek 0ml @14:57:24.15 CBV.dial between PRV and waste1 @14:57:24.47 CSV.dial :air @14:57:24.93 CS.seek 10ml @14:57:39.77 CSV.dial CTV @14:57:40.23 CS.seek 0ml @14:58:06.67 CBV.dial :waste1 @14:58:07.37 delay 5 seconds @14:58:12.46 CTV.dial :air,avoiding: :intake @14:58:13.86 Storing lyfil puck in Collection Clamp to tube 5 @14:58:14.04 SC.reconfigure :tight @14:58:14.09 SC.seek 5 @14:58:17.62 SC.configure :limp @14:58:17.82 Elbow.reconfigure :tight @14:58:17.87 Elbow.seek 5 @14:58:19.62 Forearm.reconfigure :tight @14:58:19.67 Forearm.seek 5 + 450 counts @14:58:20.69 SE.reconfigure :pick @14:58:20.75 SE.seek :beamBreak @14:58:23.58 SE.configure :default @14:58:23.74 Elbow.reconfigure :limp @14:58:23.79 Forearm.reconfigure :loose @14:58:23.83 Forearm.seek 5 @14:58:24.08 Elbow.hold @14:58:24.15 Elbow.reconfigure :tight @14:58:24.25 SE.seek 4.18 Pucks below Top Plate @14:58:24.71 Forearm.force -20 @14:58:24.75 Forearm.seek 5 + 307 counts @14:58:25.03 Forearm.reconfigure :tight @14:58:25.08 Forearm.seek 5 + 450 counts @14:58:25.29 SE.seek 5.18 Pucks below Top Plate @14:58:25.85 Forearm.reconfigure :loose @14:58:25.90 Forearm.seek :retract @14:58:26.91 Elbow.seek CC @14:58:28.62 Elbow.reconfigure :loose @14:58:29.14 Forearm.seek CC @14:58:30.16 Hand.close @14:58:30.80 CC.seek :open @14:58:35.53 Forearm.seek :retract @14:58:36.49 Elbow.reconfigure :tight @14:58:36.54 Elbow.seek 5 @14:58:38.29 Elbow.reconfigure :limp @14:58:38.34 Forearm.seek 5 @14:58:39.36 Elbow.hold @14:58:39.43 Elbow.reconfigure :tight @14:58:39.47 Hand.open @14:58:40.17 Forearm.force -20 @14:58:40.21 Forearm.seek 5 + 317 counts @14:58:40.50 Forearm.reconfigure :tight @14:58:40.55 Forearm.seek 5 + 450 counts @14:58:40.80 SE.seek :down @14:58:41.93
Forearm.reconfigure :loose @14:58:41.98 Forearm.seek :retract @14:58:43.15 SE.stop @14:58:43.19
PSV.dial :air @14:58:44.06 PS.seek 2.5ml @14:59:10.43 delay 1 second @14:59:11.44 PSV.dial :mixloop @14:59:12.04 delay 1 second @14:59:13.05 PSV.dial :air @14:59:13.51 delay 1 second @14:59:14.52 PSV.dial :mixloop @14:59:14.99 delay 1 second @14:59:16.00 PSV.dial :air @14:59:16.48 delay 1 second @14:59:17.50 PS.seek 0ml @14:59:44.02 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @14:59:44.64 Elbow.seek FlushPuck::Garage @14:59:46.48 Elbow.reconfigure :loose @14:59:46.58 Forearm.seek :garage @14:59:47.58 Hand.close @14:59:48.30 Forearm.seek :retract @14:59:49.25 Elbow.reconfigure :tight @14:59:49.30 Elbow.seek PC @14:59:50.78 Elbow.reconfigure :loose @14:59:50.88 Forearm.reconfigure :tight @14:59:50.93 Forearm.seek PC @14:59:52.12 PC.seek :closed @14:59:56.74 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.65 @14:59:56.81 Hand.open @14:59:57.48 Forearm.reconfigure :loose @14:59:57.53 Forearm.seek :retract @14:59:58.53 PRV.dial :air @14:59:59.00 PSV.dial :mixloop @14:59:59.53 PS.seek 0.3ml @15:00:03.45 PSV.dial PSR @15:00:04.53 Pulling 0.4ml of PSR.air PSR.select :air @15:00:04.56 PS.seek 0.7ml @15:00:09.42 delay 3 seconds @15:00:12.44 PSR.select :air @15:00:12.46 PS.seek 2.5ml @15:00:31.67 delay 3 seconds @15:00:34.69 PSV.dial :mixloop @15:00:35.75 PTV.dial :mixing @15:00:36.52 PBV.dial :waste2 @15:00:37.95 PS.seek 0ml @15:01:04.49 Garaging FlushPuck in Processing Clamp @15:01:04.56 Elbow.reconfigure :tight @15:01:04.61 Elbow.seek PC @15:01:04.73 Elbow.reconfigure :loose @15:01:04.83 Forearm.seek PC @15:01:05.78 Hand.close @15:01:06.43 PC.seek :open @15:01:11.17 Forearm.seek :retract @15:01:12.14 Elbow.reconfigure :tight @15:01:12.19 Elbow.seek :garage @15:01:13.51 Elbow.reconfigure :loose @15:01:13.61 Forearm.reconfigure :tight @15:01:13.66 Forearm.seek :garage @15:01:14.72 Hand.open @15:01:15.36 Forearm.reconfigure :loose @15:01:15.41 Forearm.seek :retract @15:01:16.44 Loading da puck from tube 6 into Processing Clamp @15:01:16.68 SC.reconfigure :tight @15:01:16.72 SC.seek 6 @15:01:20.35 SC.configure :limp @15:01:20.57 Elbow.reconfigure :tight @15:01:20.62 Elbow.seek 6 @15:01:22.49 Forearm.reconfigure :tight @15:01:22.54 Forearm.seek 6 + 450 counts @15:01:23.51 SE.reconfigure :pick @15:01:23.57 SE.seek :beamBreak @15:01:24.92 SE.configure :default @15:01:25.08 Elbow.seek 6 @15:01:25.70 Forearm.seek 6 + 300 counts @15:01:25.97 Forearm.reconfigure :loose @15:01:26.02 Hand.close @15:01:26.69 SE.seek :down @15:01:26.89
Forearm.seek :retract @15:01:27.68 SE.stop @15:01:27.88
Elbow.seek PC @15:01:30.45 Elbow.reconfigure :loose @15:01:30.55 Forearm.reconfigure :tight @15:01:30.60 Forearm.seek PC @15:01:31.84 PC.seek :closed @15:01:36.51 Processing Clamp onSpring/offSpring for 16/26 samples, on/off torque 1.68 @15:01:36.52 Hand.open @15:01:37.23 Forearm.reconfigure :loose @15:01:37.28 Forearm.seek :retract @15:01:38.23 Heating Puck.from 6, :da in Processing Clamp to 28C for 2:00:00 @15:01:38.25 PH.seek 28C,5:00 @15:02:23.05 PH.hold 2:00:00,28C @15:02:23.11 delay 1:30 @15:03:53.15 PSR.select :air @15:03:53.16 PSV.dial PSR @15:03:54.22 Pulling 0.5ml of PSR.wash @15:03:54.23 PSR.select :wash @15:03:54.35 PS.seek 0.5ml @15:04:00.45 delay 5 seconds @15:04:05.47 PSR.select :air @15:04:05.59 PS.seek 2ml @15:04:21.73 PTV.dial :puck @15:04:22.50 PSV.dial PTV @15:04:23.39 PBV.dial :waste1 @15:04:24.79 PS.seek 1.5ml @15:04:30.70 delay 3 seconds @15:04:33.72 PBV.dial between PSV and waste1 @15:04:34.08 PS.seek 0ml @15:04:50.23 delay 5:00 @15:09:50.25 recoverPSRreagent08 @15:09:50.26 PTV.dial :puck @15:09:50.44 PBV.dial :waste1 @15:09:50.82 delay 30 seconds @15:10:20.85 PBV.dial between PSV and waste1 @15:10:21.23 PSR.select :air @15:10:21.24 PSV.dial PSR @15:10:22.00 PS.reconfigure :fast @15:10:22.05 PS.seek 2.5ml @15:10:45.82 PSV.dial PTV @15:10:46.59 delay 5 seconds @15:10:51.60 PSV.dial PSR @15:10:52.35 delay 5 seconds @15:10:57.37 PSV.dial PTV @15:10:58.20 delay 5 seconds @15:11:03.22 PSV.dial :air @15:11:04.09 PS.seek 0ml @15:11:27.38 PTV.dial :PRVmixing,via: PRV @15:11:28.74 PSV.dial :mixloop @15:11:29.19 PRV.dial :ab1 @15:11:29.93 PS.reconfigure :slow @15:11:29.98 PS.seek 0.15ml @15:11:33.54 delay 2 seconds @15:11:35.56 CBV.dial PRV @15:11:35.58
PRV.dial CBV @15:11:36.16 PS.seek 0.35ml @15:11:40.73 delay 2 seconds @15:11:42.74 CBV.dial between PRV and PSV @15:11:43.07 PRV.dial :air @15:11:43.53 PS.seek 0.9ml @15:11:55.29 PRV.dial :waste2 @15:11:56.03 PS.seek 0.5ml @15:12:04.66 addPMLReagent08 @15:12:04.67 PSV.dial :air @15:12:05.14 PS.reconfigure :default @15:12:05.18 PS.seek 2ml @15:12:21.28 PSV.dial :PML @15:12:21.76 PTV.dial :mixing @15:12:22.50 PBV.dial :waste1 @15:12:22.94 PS.seek 1.85ml @15:12:25.28 PBV.dial between PSV and waste1 @15:12:25.65 PS.seek 0ml @15:12:45.44 delay 2 seconds @15:12:47.46 Beginning samplePassC @15:12:47.47 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @15:12:47.66 Elbow.reconfigure :tight @15:12:47.70 Elbow.seek FlushPuck::Garage @15:12:49.06 Elbow.reconfigure :loose @15:12:49.16 Forearm.seek :garage @15:12:50.12 Hand.close @15:12:50.91 Forearm.seek :retract @15:12:51.93 Elbow.reconfigure :tight @15:12:51.98 Elbow.seek CC @15:12:55.23 Elbow.reconfigure :loose @15:12:55.33 Forearm.reconfigure :tight @15:12:55.38 Forearm.seek CC @15:12:56.58 CC.seek :closed @15:13:01.16 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.46 @15:13:01.23 Hand.open @15:13:01.85 Forearm.reconfigure :loose @15:13:01.90 Forearm.seek :retract @15:13:02.90 PSV.dial PSR @15:13:04.08 Pulling 1.5ml of PSR.flush @15:13:04.09 PSR.select :flush @15:13:04.21 PS.seek 1.5ml @15:13:20.35 delay 5 seconds @15:13:25.37 PSV.dial :waste1 @15:13:25.84 PS.seek 0ml @15:13:41.94 PSV.dial PSR @15:13:42.41 PSR.select :air @15:13:42.53 PS.seek 2.5ml @15:14:08.95 PRV.dial CBV,avoiding: :ab2 @15:14:08.97
CBV.dial PRV @15:14:10.61 PSV.dial :mixloop @15:14:11.66 CTV.dial :air,avoiding: :intake @15:14:11.74 PTV.dial :PRVmixing @15:14:12.51 PS.seek 0ml @15:14:38.91 PSV.dial :air @15:14:39.51 PS.seek 2.5ml @15:15:06.03 PSV.dial :mixloop @15:15:06.49 PS.seek 0ml @15:15:32.81 PSV.dial :air @15:15:33.41 PS.seek 2.5ml @15:15:59.85 PSV.dial :mixloop @15:16:00.30 PS.seek 0ml @15:16:26.63 Garaging FlushPuck in Collection Clamp @15:16:26.70 Elbow.reconfigure :tight @15:16:26.75 Elbow.seek CC @15:16:27.08 Elbow.reconfigure :loose @15:16:27.18 Forearm.seek CC @15:16:28.19 Hand.close @15:16:28.79 CC.seek :open @15:16:33.40 Forearm.seek :retract @15:16:34.45 Elbow.reconfigure :tight @15:16:34.49 Elbow.seek :garage @15:16:37.44 Elbow.reconfigure :loose @15:16:37.54 Forearm.reconfigure :tight @15:16:37.59 Forearm.seek :garage @15:16:38.64 Hand.open @15:16:39.34 Forearm.reconfigure :loose @15:16:39.39 Forearm.seek :retract @15:16:40.35 delayUntil "15:20:47.45" @15:16:40.38 Whole Cell Archive sampling 1000ml @15:16:40.40 Loading wcr puck from tube 6 into Collection Clamp @15:16:40.64 SC.reconfigure :tight @15:16:40.69 SC.seek 6 @15:16:40.75 SC.configure :limp @15:16:40.98 Elbow.reconfigure :tight @15:16:41.03 Elbow.seek 6 @15:16:42.98 Forearm.reconfigure :tight @15:16:43.04 Forearm.seek 6 + 450 counts @15:16:44.11 SE.reconfigure :pick @15:16:44.18 SE.seek :beamBreak @15:16:46.03 SE.configure :default @15:16:46.20 Elbow.seek 6 @15:16:46.39 Forearm.seek 6 + 300 counts @15:16:46.55 Forearm.reconfigure :loose @15:16:46.60 Hand.close @15:16:47.18 SE.seek :down @15:16:47.38 Forearm.seek :retract @15:16:48.25 SE.stop @15:16:48.34 Elbow.seek CC @15:16:49.94 Elbow.reconfigure :loose @15:16:50.93 Forearm.reconfigure :tight @15:16:50.98 Forearm.seek CC @15:16:52.23 CC.seek :closed @15:16:56.75 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.22 @15:16:56.77 Hand.open @15:16:57.47 Forearm.reconfigure :loose @15:16:57.52 Forearm.seek :retract @15:16:58.57 collectSample 1000ml w/bubble point of 35psi @15:16:58.58 Priming 7ml @15:16:58.59 Intake.select :close @15:16:58.60 Exhaust.select :close @15:16:58.62 SS.hold @15:16:58.83 Calibrating pressure sensors @15:16:58.84 CTV.dial :air @15:16:58.93 SSV.dial :spit,avoiding: :exhaust @15:16:59.46 SS.reconfigure :fast @15:16:59.51 SS.seek 5ml @15:17:02.23 SSV.dial CBV @15:17:02.25 CBV.dial SSV @15:17:03.63 delay 3.8 seconds @15:17:07.58 Sensor limits: Intake max 101.3psia; Outlet max 93.1psia @15:17:07.59 SSV.dial :spit @15:17:08.94 SS.seek 0ml @15:17:11.85 SSV.dial CBV @15:17:13.20 CTV.dial :intake @15:17:13.96 SSV.dial CBV @15:17:13.98 CBV.dial SSV @15:17:14.13 Allowing 40s for 7.0ml of remaining 7.0ml @15:17:14.15 Intake.select :open @15:17:16.31 Intake @14.6psia, vacuum from 9.1 to 12.1psi @15:17:16.33 SS.reconfigure :inhaling 2.20ml/s @15:17:16.38 SS.seek 7ml @15:17:23.04 Stroke completed -- waiting for pressure to equalize ... @15:17:23.05 SS.reconfigure :exhale @15:17:23.10 SS.hold @15:17:45.57 Intake.select :close @15:17:45.73 SSV.dial :exhaust @15:17:46.49 Exhaust.select :open @15:17:46.61 SS.seek 0ml @15:17:50.97 Exhaust.select :close @15:17:51.08 Sampling 1000.0ml @15:17:51.10 Intake.select :close @15:17:51.11 Exhaust.select :close @15:17:51.12 SS.reconfigure :default @15:17:51.16 SS.hold @15:17:51.38 CTV.dial :intake @15:17:51.61 SSV.dial CBV @15:17:51.65 CBV.dial SSV @15:17:52.43 Allowing 130s for 25.0ml of remaining 1000.0ml @15:17:52.44 Intake.select :open @15:17:54.61 Intake @15.2psia, vacuum from 9.7 to 12.7psi @15:17:54.63 SS.reconfigure :inhaling 2.20ml/s @15:17:54.68 SS.seek 25ml @15:18:01.45 Vacuum too high -- waiting up to 40 seconds @15:18:01.47 SS.reconfigure :exhale @15:18:01.52 SS.hold @15:18:10.61 Intake @18.4psia, vacuum from 10.0 to 13.0psi @15:18:10.63 SS.reconfigure :inhaling 1.76ml/s @15:18:10.68 SS.seek 25ml @15:18:13.23 Vacuum too high -- waiting up to 40 seconds @15:18:13.25 SS.reconfigure :exhale @15:18:13.30 SS.hold @15:18:16.51 Intake @18.5psia, vacuum from 10.0 to 13.0psi @15:18:16.53 SS.reconfigure :inhaling 1.41ml/s @15:18:16.58 SS.seek 25ml @15:18:19.04 Vacuum too high -- waiting up to 40 seconds @15:18:19.06 SS.reconfigure :exhale @15:18:19.11 SS.hold @15:18:21.90 Intake @18.8psia, vacuum from 10.0 to 13.0psi @15:18:21.92 SS.reconfigure :inhaling 1.13ml/s @15:18:21.97 SS.seek 25ml @15:18:24.55 Vacuum too high -- waiting up to 40 seconds @15:18:24.57 SS.reconfigure :exhale @15:18:24.62 SS.hold @15:18:26.77 Intake @18.9psia, vacuum from 10.0 to 13.0psi @15:18:26.78 SS.reconfigure :inhaling 0.91ml/s @15:18:26.83 SS.seek 25ml @15:18:29.68 Vacuum too high -- waiting up to 40 seconds @15:18:29.69 SS.reconfigure :exhale @15:18:29.75 SS.hold @15:18:31.50 Intake @18.8psia, vacuum from 10.0 to 13.0psi @15:18:31.52 SS.reconfigure :inhaling 0.73ml/s @15:18:31.57 SS.seek 25ml @15:18:36.14 Vacuum too high -- waiting up to 40 seconds @15:18:36.15 SS.reconfigure :exhale @15:18:36.21 SS.hold @15:18:38.37 Intake @19.0psia, vacuum from 10.0 to 13.0psi @15:18:38.39 SS.reconfigure :inhaling 0.59ml/s @15:18:38.44 SS.seek 25ml @15:18:42.07 Stroke completed -- waiting for pressure to equalize ... @15:18:42.08 SS.reconfigure :exhale @15:18:42.13 SS.hold @15:19:12.78 Intake.select :close @15:19:12.94 SSV.dial :exhaust @15:19:13.71 Exhaust.select :open @15:19:13.83 SS.seek 0ml @15:19:22.64 Exhaust.select :close @15:19:22.75 SSV.dial CBV @15:19:22.77 CBV.dial SSV @15:19:23.57 Allowing 130s for 25.0ml of remaining 975.0ml @15:19:23.58 Intake.select :open @15:19:25.75 Intake @16.0psia, vacuum from 10.0 to 13.0psi @15:19:25.77 SS.reconfigure :inhaling 0.59ml/s @15:19:25.82 SS.seek 25ml @15:20:10.91 Stroke completed -- waiting for pressure to equalize ... @15:20:10.92 SS.reconfigure :exhale @15:20:10.97 SS.hold @15:20:42.16 Intake.select :close @15:20:42.31 SSV.dial :exhaust @15:20:43.08 Exhaust.select :open @15:20:43.20 SS.seek 0ml @15:20:47.46 recoverPRVreagent08 @15:20:47.47 PTV.dial :mixing @15:20:48.23 PBV.dial :waste1 @15:20:48.61 delay 30 seconds @15:20:52.28 Exhaust.select :close @15:20:52.39 SSV.dial CBV @15:20:52.42 CBV.dial SSV @15:20:53.21 Allowing 130s for 25.0ml of remaining 950.0ml @15:20:53.22 Intake.select :open @15:20:55.38 Intake @16.1psia, vacuum from 10.0 to 13.0psi @15:20:55.40 SS.reconfigure :inhaling 0.59ml/s @15:20:55.45 SS.seek 25ml @15:21:18.62 PBV.dial between PSV and waste1 @15:21:18.99 PSV.dial :air @15:21:19.47 PS.reconfigure :fast @15:21:19.52 PS.seek 2.5ml @15:21:39.75 Stroke completed -- waiting for pressure to equalize ... @15:21:39.76 SS.reconfigure :exhale @15:21:39.81 SS.hold @15:21:43.37 PSV.dial :mixloop @15:21:43.84 delay 2 seconds @15:21:45.86 PSV.dial :air @15:21:46.32 delay 2 seconds @15:21:48.34 PSV.dial :mixloop @15:21:48.81 delay 2 seconds @15:21:50.82 PSV.dial :air @15:21:51.30 PS.seek 0ml @15:22:11.07 Intake.select :close @15:22:11.30 SSV.dial :exhaust @15:22:12.09 Exhaust.select :open @15:22:12.24 SS.seek 0ml @15:22:14.84 washCycleDA #1 of 2 @15:22:14.85 PSR.select :air @15:22:14.87 PSV.dial PSR @15:22:16.21 Pulling 0.5ml of PSR.wash @15:22:16.22 PSR.select :wash @15:22:16.34 PS.reconfigure :default @15:22:16.39 PS.seek 0.5ml @15:22:21.08 Exhaust.select :close @15:22:21.20 SSV.dial CBV @15:22:21.22 CBV.dial SSV @15:22:22.03 Allowing 130s for 25.0ml of remaining 925.0ml @15:22:22.05 Intake.select :open @15:22:22.31 delay 5 seconds @15:22:24.21 Intake @16.1psia, vacuum from 10.0 to 13.0psi @15:22:24.23 SS.reconfigure :inhaling 0.59ml/s @15:22:24.28 SS.seek 25ml @15:22:27.32 PSR.select :air @15:22:27.44 PS.seek 2ml @15:22:43.62 PTV.dial :puck @15:22:44.37 PSV.dial PTV @15:22:45.12 PBV.dial :waste1 @15:22:45.56 PS.seek 1.5ml @15:22:51.52 delay 3 seconds @15:22:54.54 PBV.dial between PSV and waste1 @15:22:54.92 PS.seek 0ml @15:23:09.04 Stroke completed -- waiting for pressure to equalize ... @15:23:09.06 SS.reconfigure :exhale @15:23:09.10 SS.hold @15:23:11.14 delay 2:00 @15:23:36.14 Intake.select :close @15:23:36.30 SSV.dial :exhaust @15:23:37.07 Exhaust.select :open @15:23:37.19 SS.seek 0ml @15:23:46.25 Exhaust.select :close @15:23:46.36 SSV.dial CBV @15:23:46.40 CBV.dial SSV @15:23:47.15 Allowing 130s for 25.0ml of remaining 900.0ml @15:23:47.17 Intake.select :open @15:23:49.33 Intake @16.0psia, vacuum from 10.0 to 13.0psi @15:23:49.35 SS.reconfigure :inhaling 0.59ml/s @15:23:49.40 SS.seek 25ml @15:24:33.96 Stroke completed -- waiting for pressure to equalize ... @15:24:33.98 SS.reconfigure :exhale @15:24:34.02 SS.hold @15:24:58.74 Intake.select :close @15:24:58.90 SSV.dial :exhaust @15:24:59.67 Exhaust.select :open @15:24:59.79 SS.seek 0ml @15:25:08.57 Exhaust.select :close @15:25:08.69 SSV.dial CBV @15:25:08.71 CBV.dial SSV @15:25:09.50 Allowing 130s for 25.0ml of remaining 875.0ml @15:25:09.51 Intake.select :open @15:25:11.16 recoverPSRreagent08 @15:25:11.17 PTV.dial :puck @15:25:11.73 PBV.dial :waste1 @15:25:11.91 Intake @16.1psia, vacuum from 10.0 to 13.0psi @15:25:11.93 SS.reconfigure :inhaling 0.59ml/s @15:25:11.98 SS.seek 25ml @15:25:12.18 delay 30 seconds @15:25:42.20 PBV.dial between PSV and waste1 @15:25:42.58 PSR.select :air @15:25:42.60 PSV.dial PSR @15:25:43.37 PS.reconfigure :fast @15:25:43.42 PS.seek 2.5ml @15:25:56.89 Stroke completed -- waiting for pressure to equalize ... @15:25:56.90 SS.reconfigure :exhale @15:25:56.95 SS.hold @15:26:07.36 PSV.dial PTV @15:26:08.13 delay 5 seconds @15:26:13.20 PSV.dial PSR @15:26:14.07 delay 5 seconds @15:26:19.09 PSV.dial PTV @15:26:19.40 Intake.select :close @15:26:19.56 SSV.dial :exhaust @15:26:19.85 delay 5 seconds @15:26:20.32 Exhaust.select :open @15:26:20.45 SS.seek 0ml @15:26:24.87 PSV.dial :air @15:26:25.65 PS.seek 0ml @15:26:29.28 Exhaust.select :close @15:26:29.39 SSV.dial CBV @15:26:29.41 CBV.dial SSV @15:26:30.21 Allowing 130s for 25.0ml of remaining 850.0ml @15:26:30.22 Intake.select :open @15:26:32.39 Intake @16.2psia, vacuum from 10.0 to 13.0psi @15:26:32.41 SS.reconfigure :inhaling 0.59ml/s @15:26:32.46 SS.seek 25ml @15:26:49.30 washCycleDA #2 of 2 @15:26:49.31 PSR.select :air @15:26:49.33 PSV.dial PSR @15:26:50.68 Pulling 0.5ml of PSR.wash @15:26:50.69 PSR.select :wash @15:26:50.81 PS.reconfigure :default @15:26:50.85 PS.seek 0.5ml @15:26:56.86 delay 5 seconds @15:27:01.88 PSR.select :air @15:27:02.00 PS.seek 2ml @15:27:17.35 Stroke completed -- waiting for pressure to equalize ... @15:27:17.36 SS.reconfigure :exhale @15:27:17.42 SS.hold @15:27:18.11 PTV.dial :puck @15:27:18.19 PSV.dial PTV @15:27:18.94 PBV.dial :waste1 @15:27:19.37 PS.seek 1.5ml @15:27:25.39 delay 3 seconds @15:27:28.41 PBV.dial between PSV and waste1 @15:27:28.77 PS.seek 0ml @15:27:42.10 Intake.select :close @15:27:42.26 SSV.dial :exhaust @15:27:43.04 Exhaust.select :open @15:27:43.16 SS.seek 0ml @15:27:45.12 delay 2:00 @15:27:52.01 Exhaust.select :close @15:27:52.13 SSV.dial CBV @15:27:52.15 CBV.dial SSV @15:27:52.95 Allowing 130s for 25.0ml of remaining 825.0ml @15:27:52.96 Intake.select :open @15:27:55.13 Intake @16.1psia, vacuum from 10.0 to 13.0psi @15:27:55.15 SS.reconfigure :inhaling 0.59ml/s @15:27:55.20 SS.seek 25ml @15:28:39.96 Stroke completed -- waiting for pressure to equalize ... @15:28:39.97 SS.reconfigure :exhale @15:28:40.40 SS.hold @15:29:00.94 Intake.select :close @15:29:01.10 SSV.dial :exhaust @15:29:01.89 Exhaust.select :open @15:29:02.01 SS.seek 0ml @15:29:10.80 Exhaust.select :close @15:29:10.91 SSV.dial CBV @15:29:10.94 CBV.dial SSV @15:29:12.10 Allowing 130s for 25.0ml of remaining 800.0ml @15:29:12.12 Intake.select :open @15:29:14.28 Intake @16.1psia, vacuum from 10.0 to 13.0psi @15:29:14.30 SS.reconfigure :inhaling 0.59ml/s @15:29:14.35 SS.seek 25ml @15:29:45.15 recoverPSRreagent08 @15:29:45.16 PTV.dial :puck @15:29:45.24 PBV.dial :waste1 @15:29:45.60 delay 30 seconds @15:29:59.05 Stroke completed -- waiting for pressure to equalize ... @15:29:59.07 SS.reconfigure :exhale @15:29:59.11 SS.hold @15:30:15.61 PBV.dial between PSV and waste1 @15:30:15.97 PSR.select :air @15:30:15.99 PSV.dial PSR @15:30:17.14 PS.reconfigure :fast @15:30:17.26 PS.seek 2.5ml @15:30:21.58 Intake.select :close @15:30:21.74 SSV.dial :exhaust @15:30:22.51 Exhaust.select :open @15:30:22.63 SS.seek 0ml @15:30:31.66 Exhaust.select :close @15:30:31.78 SSV.dial CBV @15:30:31.80 CBV.dial SSV @15:30:32.58 Allowing 130s for 25.0ml of remaining 775.0ml @15:30:32.60 Intake.select :open @15:30:34.81 Intake @16.1psia, vacuum from 10.0 to 13.0psi @15:30:34.83 SS.reconfigure :inhaling 0.59ml/s @15:30:34.88 SS.seek 25ml @15:30:41.45 PSV.dial PTV @15:30:42.34 delay 5 seconds @15:30:47.37 PSV.dial PSR @15:30:48.11 delay 5 seconds @15:30:53.13 PSV.dial PTV @15:30:54.02 delay 5 seconds @15:30:59.04 PSV.dial :air @15:30:59.82 PS.seek 0ml @15:31:19.36 Stroke completed -- waiting for pressure to equalize ... @15:31:19.38 SS.reconfigure :exhale @15:31:19.42 SS.hold @15:31:23.33 getPMLReagent08 @15:31:23.34 PRV.dial :air @15:31:23.78 delay 5 seconds @15:31:29.30 PTV.dial :PRVmixing @15:31:30.66 delay 5 seconds @15:31:35.68 PRV.dial :ab2 @15:31:36.15 PTV.dial :PRVmixing @15:31:36.23 PSV.dial :mixloop @15:31:36.71 PS.reconfigure :default @15:31:36.76 PS.seek 0.3ml @15:31:40.74 delay 2 seconds @15:31:42.76 PRV.dial :air @15:31:43.29 PS.seek 0.55ml @15:31:46.10 Intake.select :close @15:31:46.26 SSV.dial :exhaust @15:31:46.64 addPMLReagent08 @15:31:46.65 PSV.dial :air @15:31:47.02 Exhaust.select :open @15:31:47.13 PS.seek 2ml @15:31:47.19 SS.seek 0ml @15:31:55.99 Exhaust.select :close @15:31:56.10 SSV.dial CBV @15:31:56.12 CBV.dial SSV @15:31:56.93 Allowing 130s for 25.0ml of remaining 750.0ml @15:31:56.94 Intake.select :open @15:31:59.57 Intake @16.1psia, vacuum from 10.0 to 13.0psi @15:31:59.59 SS.reconfigure :inhaling 0.59ml/s @15:31:59.64 SS.seek 25ml @15:32:02.79 PSV.dial :PML @15:32:03.26 PTV.dial :mixing @15:32:04.03 PBV.dial :waste1 @15:32:04.46 PS.seek 1.85ml @15:32:06.83 PBV.dial between PSV and waste1 @15:32:07.21 PS.seek 0ml @15:32:26.92 delay 2 seconds @15:32:28.94 delay 8:00 @15:32:44.16 Vacuum too high -- waiting up to 40 seconds @15:32:44.17 SS.reconfigure :exhale @15:32:44.23 SS.hold @15:32:54.15 Intake @17.3psia, vacuum from 10.0 to 13.0psi @15:32:54.17 SS.reconfigure :inhaling 0.47ml/s @15:32:54.22 SS.seek 25ml @15:32:54.88 Stroke completed -- waiting for pressure to equalize ... @15:32:54.89 SS.reconfigure :exhale @15:32:54.94 SS.hold @15:33:09.67 Intake.select :close @15:33:09.83 SSV.dial :exhaust @15:33:10.60 Exhaust.select :open @15:33:10.73 SS.seek 0ml @15:33:19.96 Exhaust.select :close @15:33:20.06 SSV.dial CBV @15:33:20.11 CBV.dial SSV @15:33:20.85 Allowing 130s for 25.0ml of remaining 725.0ml @15:33:20.86 Intake.select :open @15:33:23.03 Intake @16.2psia, vacuum from 10.0 to 13.0psi @15:33:23.05 SS.reconfigure :inhaling 0.47ml/s @15:33:23.10 SS.seek 25ml @15:34:18.20 Stroke completed -- waiting for pressure to equalize ... @15:34:18.22 SS.reconfigure :exhale @15:34:18.26 SS.hold @15:34:39.15 Intake.select :close @15:34:39.31 SSV.dial :exhaust @15:34:40.10 Exhaust.select :open @15:34:40.22 SS.seek 0ml @15:34:49.25 Exhaust.select :close @15:34:49.36 SSV.dial CBV @15:34:49.40 CBV.dial SSV @15:34:50.17 Allowing 130s for 25.0ml of remaining 700.0ml @15:34:50.19 Intake.select :open @15:34:52.82 Intake @16.1psia, vacuum from 10.0 to 13.0psi @15:34:52.84 SS.reconfigure :inhaling 0.47ml/s @15:34:52.89 SS.seek 25ml @15:35:47.92 Stroke completed -- waiting for pressure to equalize ... @15:35:47.93 SS.reconfigure :exhale @15:35:47.98 SS.hold @15:36:10.47 Intake.select :close @15:36:11.02 SSV.dial :exhaust @15:36:11.88 Exhaust.select :open @15:36:11.99 SS.seek 0ml @15:36:20.76 Exhaust.select :close @15:36:20.87 SSV.dial CBV @15:36:20.92 CBV.dial SSV @15:36:21.67 Allowing 130s for 25.0ml of remaining 675.0ml @15:36:21.69 Intake.select :open @15:36:23.85 Intake @16.1psia, vacuum from 10.0 to 13.0psi @15:36:23.87 SS.reconfigure :inhaling 0.47ml/s @15:36:23.92 SS.seek 25ml @15:37:19.45 Stroke completed -- waiting for pressure to equalize ... @15:37:19.46 SS.reconfigure :exhale @15:37:19.51 SS.hold @15:37:44.53 Intake.select :close @15:37:44.69 SSV.dial :exhaust @15:37:45.45 Exhaust.select :open @15:37:45.57 SS.seek 0ml @15:37:54.39 Exhaust.select :close @15:37:54.50 SSV.dial CBV @15:37:54.53 CBV.dial SSV @15:37:55.31 Allowing 130s for 25.0ml of remaining 650.0ml @15:37:55.32 Intake.select :open @15:37:57.49 Intake @16.1psia, vacuum from 10.0 to 13.0psi @15:37:57.51 SS.reconfigure :inhaling 0.47ml/s @15:37:57.56 SS.seek 25ml @15:38:53.04 Stroke completed -- waiting for pressure to equalize ... @15:38:53.05 SS.reconfigure :exhale @15:38:53.10 SS.hold @15:39:17.66 Intake.select :close @15:39:17.83 SSV.dial :exhaust @15:39:18.59 Exhaust.select :open @15:39:18.71 SS.seek 0ml @15:39:27.56 Exhaust.select :close @15:39:27.67 SSV.dial CBV @15:39:27.70 CBV.dial SSV @15:39:28.48 Allowing 130s for 25.0ml of remaining 625.0ml @15:39:28.49 Intake.select :open @15:39:30.66 Intake @16.3psia, vacuum from 10.0 to 13.0psi @15:39:30.68 SS.reconfigure :inhaling 0.47ml/s @15:39:30.73 SS.seek 25ml @15:40:26.43 Stroke completed -- waiting for pressure to equalize ... @15:40:26.44 SS.reconfigure :exhale @15:40:26.49 SS.hold @15:40:28.98 recoverPRVreagent08 @15:40:28.99 PTV.dial :mixing @15:40:29.00 PBV.dial :waste1 @15:40:29.53 delay 30 seconds @15:40:53.23 Intake.select :close @15:40:53.39 SSV.dial :exhaust @15:40:54.16 Exhaust.select :open @15:40:54.28 SS.seek 0ml @15:40:59.55 PBV.dial between PSV and waste1 @15:40:59.93 PSV.dial :air @15:41:00.40 PS.reconfigure :fast @15:41:00.45 PS.seek 2.5ml @15:41:03.07 Exhaust.select :close @15:41:03.19 SSV.dial CBV @15:41:03.21 CBV.dial SSV @15:41:04.02 Allowing 130s for 25.0ml of remaining 600.0ml @15:41:04.04 Intake.select :open @15:41:06.21 Intake @16.2psia, vacuum from 10.0 to 13.0psi @15:41:06.23 SS.reconfigure :inhaling 0.47ml/s @15:41:06.74 SS.seek 25ml @15:41:24.56 PSV.dial :mixloop @15:41:25.04 delay 2 seconds @15:41:27.05 PSV.dial :air @15:41:27.52 delay 2 seconds @15:41:29.54 PSV.dial :mixloop @15:41:30.00 delay 2 seconds @15:41:32.02 PSV.dial :air @15:41:32.49 PS.seek 0ml @15:41:55.97 washCycleDA #1 of 3 @15:41:55.98 PSR.select :air @15:41:56.00 PSV.dial PSR @15:41:57.35 Pulling 0.5ml of PSR.wash @15:41:57.36 PSR.select :wash @15:41:57.47 PS.reconfigure :default @15:41:57.52 PS.seek 0.5ml @15:42:02.37 Stroke completed -- waiting for pressure to equalize ... @15:42:02.38 SS.reconfigure :exhale @15:42:02.43 SS.hold @15:42:03.50 delay 5 seconds @15:42:08.52 PSR.select :air @15:42:08.63 PS.seek 2ml @15:42:24.72 PTV.dial :puck @15:42:25.47 PSV.dial PTV @15:42:26.22 PBV.dial :waste1 @15:42:26.66 PS.seek 1.5ml @15:42:32.56 delay 3 seconds @15:42:33.08 Intake.select :close @15:42:33.24 SSV.dial :exhaust @15:42:34.02 Exhaust.select :open @15:42:34.14 SS.seek 0ml @15:42:35.58 PBV.dial between PSV and waste1 @15:42:35.97 PS.seek 0ml @15:42:42.98 Exhaust.select :close @15:42:43.09 SSV.dial CBV @15:42:43.11 CBV.dial SSV @15:42:43.91 Allowing 130s for 25.0ml of remaining 575.0ml @15:42:43.93 Intake.select :open @15:42:46.10 Intake @16.3psia, vacuum from 10.0 to 13.0psi @15:42:46.12 SS.reconfigure :inhaling 0.47ml/s @15:42:46.17 SS.seek 25ml @15:42:52.16 delay 2:00 @15:43:36.27 Vacuum too high -- waiting up to 40 seconds @15:43:36.28 SS.reconfigure :exhale @15:43:36.34 SS.hold @15:43:50.91 Intake @17.9psia, vacuum from 10.0 to 13.0psi @15:43:50.93 SS.reconfigure :inhaling 0.38ml/s @15:43:50.98 SS.seek 25ml @15:43:58.08 Stroke completed -- waiting for pressure to equalize ... @15:43:58.09 SS.reconfigure :exhale @15:43:58.14 SS.hold @15:44:16.56 Intake.select :close @15:44:16.71 SSV.dial :exhaust @15:44:17.49 Exhaust.select :open @15:44:17.61 SS.seek 0ml @15:44:26.36 Exhaust.select :close @15:44:26.47 SSV.dial CBV @15:44:26.50 CBV.dial SSV @15:44:27.30 Allowing 130s for 25.0ml of remaining 550.0ml @15:44:27.32 Intake.select :open @15:44:29.48 Intake @16.3psia, vacuum from 10.0 to 13.0psi @15:44:29.50 SS.reconfigure :inhaling 0.38ml/s @15:44:29.55 SS.seek 25ml @15:44:52.19 recoverPSRreagent08 @15:44:52.20 PTV.dial :puck @15:44:52.28 PBV.dial :waste1 @15:44:52.66 delay 30 seconds @15:45:22.68 PBV.dial between PSV and waste1 @15:45:23.06 PSR.select :air @15:45:23.08 PSV.dial PSR @15:45:23.85 PS.reconfigure :fast @15:45:23.90 PS.seek 2.5ml @15:45:37.83 Stroke completed -- waiting for pressure to equalize ... @15:45:37.84 SS.reconfigure :exhale @15:45:37.89 SS.hold @15:45:47.83 PSV.dial PTV @15:45:48.62 delay 5 seconds @15:45:53.64 PSV.dial PSR @15:45:54.41 delay 5 seconds @15:45:59.42 PSV.dial PTV @15:46:00.19 delay 5 seconds @15:46:05.20 PSV.dial :air @15:46:05.97 PS.seek 0ml @15:46:06.84 Intake.select :close @15:46:07.00 SSV.dial :exhaust @15:46:08.26 Exhaust.select :open @15:46:08.38 SS.seek 0ml @15:46:17.46 Exhaust.select :close @15:46:17.57 SSV.dial CBV @15:46:17.59 CBV.dial SSV @15:46:18.38 Allowing 130s for 25.0ml of remaining 525.0ml @15:46:18.39 Intake.select :open @15:46:20.56 Intake @16.3psia, vacuum from 10.0 to 13.0psi @15:46:20.58 SS.reconfigure :inhaling 0.38ml/s @15:46:20.63 SS.seek 25ml @15:46:29.62 washCycleDA #2 of 3 @15:46:29.63 PSR.select :air @15:46:29.65 PSV.dial PSR @15:46:31.00 Pulling 0.5ml of PSR.wash @15:46:31.01 PSR.select :wash @15:46:31.12 PS.reconfigure :default @15:46:31.17 PS.seek 0.5ml @15:46:37.06 delay 5 seconds @15:46:42.08 PSR.select :air @15:46:42.20 PS.seek 2ml @15:46:58.43 PTV.dial :puck @15:46:58.51 PSV.dial PTV @15:46:59.26 PBV.dial :waste1 @15:46:59.71 PS.seek 1.5ml @15:47:05.69 delay 3 seconds @15:47:08.71 PBV.dial between PSV and waste1 @15:47:09.08 PS.seek 0ml @15:47:25.22 delay 2:00 @15:47:28.37 Stroke completed -- waiting for pressure to equalize ... @15:47:28.39 SS.reconfigure :exhale @15:47:28.44 SS.hold @15:47:59.20 Intake.select :close @15:47:59.36 SSV.dial :exhaust @15:48:00.13 Exhaust.select :open @15:48:00.25 SS.seek 0ml @15:48:09.11 Exhaust.select :close @15:48:09.22 SSV.dial CBV @15:48:09.24 CBV.dial SSV @15:48:10.03 Allowing 130s for 25.0ml of remaining 500.0ml @15:48:10.05 Intake.select :open @15:48:12.21 Intake @16.4psia, vacuum from 10.0 to 13.0psi @15:48:12.23 SS.reconfigure :inhaling 0.38ml/s @15:48:12.28 SS.seek 25ml @15:49:21.28 Stroke completed -- waiting for pressure to equalize ... @15:49:21.29 SS.reconfigure :exhale @15:49:21.34 SS.hold @15:49:25.24 recoverPSRreagent08 @15:49:25.25 PTV.dial :puck @15:49:25.36 PBV.dial :waste1 @15:49:25.79 delay 30 seconds @15:49:54.57 Intake.select :close @15:49:54.73 SSV.dial :exhaust @15:49:55.81 PBV.dial between PSV and waste1 @15:49:56.03 Exhaust.select :open @15:49:56.15 SS.seek 0ml @15:49:56.22 PSR.select :air @15:49:56.26 PSV.dial PSR @15:49:57.03 PS.reconfigure :fast @15:49:57.08 PS.seek 2.5ml @15:50:05.25 Exhaust.select :close @15:50:05.36 SSV.dial CBV @15:50:05.41 CBV.dial SSV @15:50:06.16 Allowing 130s for 25.0ml of remaining 475.0ml @15:50:06.17 Intake.select :open @15:50:08.34 Intake @16.3psia, vacuum from 10.0 to 13.0psi @15:50:08.36 SS.reconfigure :inhaling 0.38ml/s @15:50:08.41 SS.seek 25ml @15:50:21.12 PSV.dial PTV @15:50:22.01 delay 5 seconds @15:50:27.03 PSV.dial PSR @15:50:27.78 delay 5 seconds @15:50:32.80 PSV.dial PTV @15:50:33.57 delay 5 seconds @15:50:38.59 PSV.dial :air @15:50:39.36 PS.seek 0ml @15:51:02.65 washCycleDA #3 of 3 @15:51:02.66 PSR.select :air @15:51:02.68 PSV.dial PSR @15:51:04.02 Pulling 0.5ml of PSR.wash @15:51:04.04 PSR.select :wash @15:51:04.16 PS.reconfigure :default @15:51:04.21 PS.seek 0.5ml @15:51:10.15 delay 5 seconds @15:51:11.76 Vacuum too high -- waiting up to 40 seconds @15:51:11.77 SS.reconfigure :exhale @15:51:11.83 SS.hold @15:51:15.17 PSR.select :air @15:51:15.29 PS.seek 2ml @15:51:28.33 Intake @17.5psia, vacuum from 10.0 to 13.0psi @15:51:28.34 SS.reconfigure :inhaling 0.31ml/s @15:51:28.40 SS.seek 25ml @15:51:31.44 PTV.dial :puck @15:51:31.51 PSV.dial PTV @15:51:32.26 PBV.dial :waste1 @15:51:32.69 PS.seek 1.5ml @15:51:34.29 Stroke completed -- waiting for pressure to equalize ... @15:51:34.31 SS.reconfigure :exhale @15:51:34.35 SS.hold @15:51:38.65 delay 3 seconds @15:51:41.67 PBV.dial between PSV and waste1 @15:51:42.05 PS.seek 0ml @15:51:55.26 Intake.select :close @15:51:55.42 SSV.dial :exhaust @15:51:56.18 Exhaust.select :open @15:51:56.35 SS.seek 0ml @15:51:58.25 delay 2:00 @15:52:05.17 Exhaust.select :close @15:52:05.28 SSV.dial CBV @15:52:05.33 CBV.dial SSV @15:52:06.07 Allowing 130s for 25.0ml of remaining 450.0ml @15:52:06.09 Intake.select :open @15:52:08.26 Intake @16.4psia, vacuum from 10.0 to 13.0psi @15:52:08.28 SS.reconfigure :inhaling 0.31ml/s @15:52:08.33 SS.seek 25ml @15:53:34.41 Stroke completed -- waiting for pressure to equalize ... @15:53:34.43 SS.reconfigure :exhale @15:53:34.47 SS.hold @15:53:58.27 recoverPSRreagent08 @15:53:58.28 PTV.dial :puck @15:53:58.35 PBV.dial :waste1 @15:53:58.73 delay 30 seconds @15:54:05.29 Intake.select :close @15:54:05.45 SSV.dial :exhaust @15:54:06.21 Exhaust.select :open @15:54:06.33 SS.seek 0ml @15:54:15.42 Exhaust.select :close @15:54:15.52 SSV.dial CBV @15:54:15.56 CBV.dial SSV @15:54:16.32 Allowing 130s for 25.0ml of remaining 425.0ml @15:54:16.34 Intake.select :open @15:54:18.50 Intake @16.5psia, vacuum from 10.0 to 13.0psi @15:54:18.52 SS.reconfigure :inhaling 0.31ml/s @15:54:18.57 SS.seek 25ml @15:54:28.75 PBV.dial between PSV and waste1 @15:54:29.13 PSR.select :air @15:54:29.15 PSV.dial PSR @15:54:29.92 PS.reconfigure :fast @15:54:29.97 PS.seek 2.5ml @15:54:54.05 PSV.dial PTV @15:54:54.82 delay 5 seconds @15:54:59.84 PSV.dial PSR @15:55:00.60 delay 5 seconds @15:55:05.62 PSV.dial PTV @15:55:06.40 delay 5 seconds @15:55:11.42 PSV.dial :air @15:55:12.20 PS.seek 0ml @15:55:35.52 Gate.power :camera,:ON @15:55:35.54 Mixing DA Substrates @15:55:35.55 PSR.select :air @15:55:35.57 PSV.dial PSR @15:55:36.99 Pulling 0.25ml of PSR.sub1 @15:55:37.01 PSR.select :sub1 @15:55:37.22 PS.reconfigure :default @15:55:37.33 PS.seek 0.25ml @15:55:40.78 delay 5 seconds @15:55:44.90 Stroke completed -- waiting for pressure to equalize ... @15:55:44.91 SS.reconfigure :exhale @15:55:44.96 SS.hold @15:55:45.81 Pulling 0.25ml of PSR.sub2 @15:55:45.82 PSR.select :sub2 @15:55:45.93 PS.seek 0.5ml @15:55:49.27 delay 5 seconds @15:55:54.28 PSR.select :air @15:55:54.40 PS.seek 2ml @15:56:10.61 PTV.dial :puck @15:56:10.69 PSV.dial PTV @15:56:11.47 PBV.dial :waste1 @15:56:11.99 PS.seek 1.5ml @15:56:18.00 delay 3 seconds @15:56:21.03 PBV.dial between PSV and waste1 @15:56:21.43 PS.seek 0ml @15:56:22.15 Intake.select :close @15:56:22.31 SSV.dial :exhaust @15:56:23.07 Exhaust.select :open @15:56:23.19 SS.seek 0ml @15:56:32.04 Exhaust.select :close @15:56:32.15 SSV.dial CBV @15:56:32.17 CBV.dial SSV @15:56:32.98 Allowing 130s for 25.0ml of remaining 400.0ml @15:56:32.99 Intake.select :open @15:56:35.64 Intake @16.4psia, vacuum from 10.0 to 13.0psi @15:56:35.66 SS.reconfigure :inhaling 0.31ml/s @15:56:35.71 SS.seek 25ml @15:56:37.59 delay 10 seconds @15:56:47.62 recoverPSRreagent08 @15:56:47.63 PTV.dial :puck @15:56:47.71 PBV.dial :waste1 @15:56:48.08 delay 30 seconds @15:57:18.11 PBV.dial between PSV and waste1 @15:57:18.49 PSR.select :air @15:57:18.50 PSV.dial PSR @15:57:19.29 PS.reconfigure :fast @15:57:19.34 PS.seek 2.5ml @15:57:43.64 PSV.dial PTV @15:57:44.42 delay 5 seconds @15:57:49.45 PSV.dial PSR @15:57:50.22 delay 5 seconds @15:57:55.24 PSV.dial PTV @15:57:56.02 delay 5 seconds @15:58:01.04 PSV.dial :air @15:58:01.82 PS.seek 0ml @15:58:03.02 Stroke completed -- waiting for pressure to equalize ... @15:58:03.03 SS.reconfigure :exhale @15:58:03.08 SS.hold @15:58:25.99 Additional air clear @15:58:26.00 PSR.select :air @15:58:26.01 PSV.dial PSR @15:58:27.38 Pulling 2ml of PSR.air @15:58:27.39 PSR.select :air @15:58:27.41 PS.reconfigure :default @15:58:27.45 PS.seek 2ml @15:58:43.00 SS.reconfigure :default @15:58:43.05 SS.hold @15:58:43.12 Intake.select :close @15:58:43.28 ShallowSampler::Clogged in WCR -- Filtering too Slowly after 625.0ml @15:58:43.29 SSV.dial :exhaust @15:58:44.07 Exhaust.select :open @15:58:44.18 SS.reconfigure :exhale @15:58:44.23 SS.seek 0ml @15:58:48.67 delay 5 seconds @15:58:53.33 Exhaust.select :close @15:58:53.44 Sampled 625.0ml @15:58:53.46 Drying puck before fixing for 5:00 @15:58:53.47 CBV.dial :waste2b @15:58:53.69 PTV.dial :puck @15:58:53.77 PSV.dial PTV @15:58:54.24 delay 1:00 @15:58:54.53 PBV.dial :waste1 @15:58:54.96 PS.seek 1.5ml @15:59:00.92 delay 3 seconds @15:59:03.94 PBV.dial between PSV and waste1 @15:59:04.31 delay 1 second @15:59:05.33 PSV.dial :waste1 @15:59:06.91 PS.seek 0ml @15:59:23.07 Processing Puck Heater at 28.1C before imaging @15:59:23.08 PH.coast @15:59:23.20 Imaging da puck in Processing Clamp while storing it to tube 5 @15:59:23.37 SC.reconfigure :tight @15:59:23.42 SC.seek 5 @15:59:27.05 SC.configure :limp @15:59:27.26 Elbow.reconfigure :tight @15:59:27.32 Elbow.seek 5 @15:59:29.07 Forearm.reconfigure :tight @15:59:29.12 Forearm.seek 5 + 450 counts @15:59:30.15 SE.reconfigure :pick @15:59:30.21 SE.seek :beamBreak @15:59:32.97 SE.configure :default @15:59:33.13 Elbow.reconfigure :limp @15:59:33.18 Forearm.reconfigure :loose @15:59:33.23 Forearm.seek 5 @15:59:33.42 Elbow.hold @15:59:33.49 Elbow.reconfigure :tight @15:59:33.59 SE.seek 5.17 Pucks below Top Plate @15:59:34.15 Forearm.force -20 @15:59:34.20 Forearm.seek 5 + 311 counts @15:59:34.48 Forearm.reconfigure :tight @15:59:34.53 Forearm.seek 5 + 450 counts @15:59:34.73 SE.seek 6.17 Pucks below Top Plate @15:59:35.26 Forearm.reconfigure :loose @15:59:35.31 Forearm.seek :retract @15:59:36.24 Elbow.seek PC @15:59:38.76 Elbow.reconfigure :loose @15:59:38.86 Forearm.seek PC @15:59:39.84 Hand.close @15:59:40.51 PC.seek :open @15:59:45.35 Forearm.seek :retract @15:59:46.35 Elbow.reconfigure :tight @15:59:46.40 Elbow.seek 5 @15:59:49.01 Forearm.reconfigure :tight @15:59:49.06 Forearm.seek 5 + 450 counts @15:59:49.99 Hand.open @15:59:50.70 SE.seek 5.82 Pucks below Top Plate @15:59:50.97 SE.reconfigure :seal @15:59:51.02 SE.seek 4.86 Pucks below Top Plate @15:59:51.58 Lights, camera, ACTION! @15:59:51.61 PuckCamera: snap -bin=2,2 -auto /var/log/esp/da23dec0815h1000ml.tif @15:59:54.27 CSV.dial :air @15:59:54.78 CS.reconfigure :fast @15:59:54.84 CS.seek 10ml @16:00:11.07 Waiting >20 seconds for da to relinquish FlushPuck @16:00:12.71 SX HX9/16 (USB:22): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 17.762 seconds /var/log/esp/da23dec0815h1000ml.tif: TIFF Upload Complete @16:00:12.74 PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da23dec0815h1000ml15s.tif @16:00:19.94 Pumping wcr puck from tube 6 up to 35psi... @16:00:19.96 CBV.dial SSV @16:00:21.25 CBV.dial between CSV and waste2b @16:00:22.25 CSV.dial between air and CTV @16:00:22.59 CS.seek 6.919ml @16:00:28.45 SX HX9/16 (USB:22): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15 seconds /var/log/esp/midres/da23dec0815h1000ml15s.tif: TIFF Upload Complete @16:00:28.48 PuckCamera: snap -bin=2,2 20.0 /var/log/esp/midres/da23dec0815h1000ml20s.tif @16:00:30.70 CTV.dial CSV,avoiding: :air @16:00:30.72 CSV.dial CTV @16:00:31.61 CS.reconfigure :pressurize @16:00:31.67 CS.seek 0ml @16:00:46.33 CBV.dial :waste2b @16:00:46.68 CS.reconfigure :default @16:00:46.74 CS.seek 0ml @16:00:49.11 SX HX9/16 (USB:22): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 20 seconds /var/log/esp/midres/da23dec0815h1000ml20s.tif: TIFF Upload Complete @16:00:49.12 Gate.power :camera,:OFF @16:00:49.15 DAfans.email "17.762 sec auto-exposure",Subject: "da23dec0815h1000ml images!" @16:00:49.58 Elbow.seek 5 @16:00:49.80 Forearm.seek 5 + 450 counts @16:00:49.88 SE.reconfigure :gently @16:00:50.52 SE.seek 5.58 Pucks below Top Plate @16:00:51.16 Elbow.reconfigure :limp @16:00:51.21 Forearm.reconfigure :loose @16:00:51.28 Forearm.seek 5 @16:00:51.47 delay 1:00 @16:00:51.71 Elbow.hold @16:00:51.79 Elbow.reconfigure :tight @16:00:51.85 SE.reconfigure :default @16:00:51.89 SE.seek 5.76 Pucks below Top Plate @16:00:52.24 Forearm.force -20 @16:00:52.28 Forearm.seek 5 + 285 counts @16:00:52.62 Forearm.reconfigure :tight @16:00:52.67 Forearm.seek 5 + 450 counts @16:00:52.93 SE.seek :down @16:00:54.09 Forearm.reconfigure :loose @16:00:54.15 Forearm.seek :retract @16:00:55.26 SE.stop @16:00:55.48 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @16:00:55.91 Elbow.seek FlushPuck::Garage @16:00:57.92 Elbow.reconfigure :loose @16:00:58.14 Forearm.seek :garage @16:00:59.15 Hand.close @16:01:00.04 Forearm.seek :retract @16:01:01.08 Elbow.reconfigure :tight @16:01:01.19 Elbow.seek PC @16:01:02.76 Elbow.reconfigure :loose @16:01:03.05 Forearm.reconfigure :tight @16:01:03.17 Forearm.seek PC @16:01:05.83 PC.seek :closed @16:01:10.61 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.65 @16:01:10.74 Hand.open @16:01:11.52 Forearm.reconfigure :loose @16:01:11.61 Forearm.seek :retract @16:01:12.59 PSR.select :air @16:01:12.63 PSV.dial PSR @16:01:13.15 Pulling 0.5ml of PSR.flush @16:01:13.17 PSR.select :flush @16:01:13.35 PS.seek 0.5ml @16:01:19.29 delay 5 seconds @16:01:22.15 upsync @16:01:24.32 PSR.select :air @16:01:24.44 PS.seek 2.5ml @16:01:45.67 delay 1 second @16:01:46.69 PRV.dial :waste2,avoiding: :ab2 @16:01:48.57 PTV.dial :PRVmixing @16:01:49.90 PSV.dial :mixloop @16:01:50.95 PS.seek :empty @16:01:51.50 CTV.dial :air,avoiding: :intake @16:01:52.84 Fix #1 of 2 (with RNAlater) for 2:30 @16:01:52.85 CSV.dial CSR @16:01:53.29 Pulling 2ml of CSR.RNAlater @16:01:53.30 CSR.select :RNAlater @16:01:53.42 CS.seek 2ml @16:01:59.30 delay 5 seconds @16:02:04.32 CSR.select :air @16:02:04.44 CS.reconfigure :fast @16:02:04.49 CS.seek 10ml @16:02:17.23 PSR.select :air @16:02:17.25 PSV.dial PSR @16:02:18.31 Pulling 0.5ml of PSR.flush @16:02:18.32 PSR.select :flush @16:02:18.43 PS.seek 0.5ml @16:02:24.21 Pumping wcr puck from tube 6 up to 35psi... @16:02:24.23 CBV.dial SSV @16:02:24.42 delay 5 seconds @16:02:25.48 CBV.dial between CSV and waste2b @16:02:26.46 CSV.dial between air and CTV @16:02:27.06 CS.seek 6.98ml @16:02:29.44 PSR.select :air @16:02:29.56 PS.seek 2.5ml @16:02:35.28 CTV.dial CSV,avoiding: :air @16:02:35.30 CSV.dial CTV @16:02:36.70 CS.reconfigure :pressurize @16:02:36.75 CS.seek 0ml @16:02:50.89 delay 1 second @16:02:51.91 PBV.dial :waste1 @16:02:52.28 PTV.dial :mixing @16:02:53.03 PSV.dial :mixloop @16:02:54.07 delay 2:30 @16:02:54.22 PS.seek :empty @16:03:20.67 PSV.dial PSR @16:03:21.79 PSR.select :flush @16:03:21.92 PS.seek 1ml @16:03:33.01 PSR.select :air @16:03:33.13 PS.seek 2.5ml @16:03:49.23 PSV.dial PTV @16:03:49.99 PTV.dial :puck @16:03:50.74 PBV.dial :waste1 @16:03:50.83 PS.seek 0ml @16:04:17.20 PSV.dial PSR @16:04:18.00 PSR.select :flush @16:04:18.13 PS.seek 0.5ml @16:04:24.10 PSR.select :air @16:04:24.22 PS.seek 2.5ml @16:04:45.51 PSV.dial PTV @16:04:46.27 PBV.dial PSV @16:04:46.97 PS.seek 0ml @16:05:13.51 PSV.dial PBV @16:05:13.99 PS.seek 2.5ml @16:05:24.10 CBV.dial :waste2b @16:05:24.43 CS.reconfigure :default @16:05:24.48 CS.seek 0ml @16:05:26.30 delay 1:00 @16:05:40.35 PSV.dial :waste1 @16:05:41.11 PS.seek 0ml @16:06:07.63 PSV.dial PSR @16:06:08.10 PSR.select :air @16:06:08.13 PS.seek 2.5ml @16:06:26.32 CTV.dial :air,avoiding: :intake @16:06:27.66 Fix #2 of 2 (with RNAlater) for 2:30 @16:06:27.68 CSV.dial CSR @16:06:28.56 Pulling 2ml of CSR.RNAlater @16:06:28.57 CSR.select :RNAlater @16:06:28.69 CS.seek 2ml @16:06:34.54 PSV.dial PTV @16:06:34.86 delay 5 seconds @16:06:35.40 PBV.dial PSV @16:06:35.48 PS.seek 0ml @16:06:39.88 CSR.select :air @16:06:39.99 CS.reconfigure :fast @16:06:40.05 CS.seek 10ml @16:06:59.80 Pumping wcr puck from tube 6 up to 35psi... @16:06:59.82 CBV.dial SSV @16:07:01.09 CBV.dial between CSV and waste2b @16:07:01.88 PSV.dial PBV @16:07:02.06 CSV.dial between air and CTV @16:07:02.34 PS.seek 2.5ml @16:07:02.66 CS.seek 6.984ml @16:07:10.36 CTV.dial CSV,avoiding: :air @16:07:10.39 CSV.dial CTV @16:07:11.77 CS.reconfigure :pressurize @16:07:11.82 CS.seek 0ml @16:07:25.19 delay 2:30 @16:07:28.66 PSV.dial :waste1 @16:07:29.42 PS.seek 0ml @16:07:55.75 PSV.dial PSR @16:07:56.22 PSR.select :flush @16:07:56.35 PS.seek 1ml @16:08:07.47 PSR.select :air @16:08:07.59 PS.seek 2.5ml @16:08:23.78 PSV.dial PTV @16:08:24.64 PTV.dial :puck @16:08:24.66 PBV.dial :waste1 @16:08:25.21 PS.seek 0ml @16:08:51.57 PSV.dial PSR @16:08:52.32 PSR.select :flush @16:08:52.43 PS.seek 0.5ml @16:08:58.38 PSR.select :air @16:08:58.50 PS.seek 2.5ml @16:09:19.69 PSV.dial PTV @16:09:20.46 PBV.dial PSV @16:09:21.48 PS.seek 0ml @16:09:47.82 PSV.dial PBV @16:09:48.29 PS.seek 2.5ml @16:09:55.21 CBV.dial :waste2b @16:09:55.57 CS.reconfigure :default @16:09:55.62 CS.seek 0ml @16:10:01.30 delay 1:00 @16:10:14.69 PSV.dial :waste1 @16:10:15.43 PS.seek 0ml @16:10:41.78 PSV.dial PSR @16:10:42.25 PSR.select :air @16:10:42.28 PS.seek 2.5ml @16:11:01.32 CTV.dial :air,avoiding: :intake @16:11:02.71 Storing wcr puck in Collection Clamp to tube 5 @16:11:03.13 Elbow.reconfigure :tight @16:11:03.19 Elbow.seek 5 @16:11:05.74 Forearm.reconfigure :tight @16:11:05.79 Forearm.seek 5 + 450 counts @16:11:06.80 SE.reconfigure :pick @16:11:06.86 SE.seek :beamBreak @16:11:08.63 PSV.dial PTV @16:11:09.38 PBV.dial PSV @16:11:09.60 PS.seek 0ml @16:11:09.62 SE.configure :default @16:11:09.84 Elbow.reconfigure :limp @16:11:09.89 Forearm.reconfigure :loose @16:11:09.94 Forearm.seek 5 @16:11:10.18 Elbow.hold @16:11:10.25 Elbow.reconfigure :tight @16:11:10.35 SE.seek 6.16 Pucks below Top Plate @16:11:11.29 Forearm.force -20 @16:11:11.34 Forearm.seek 5 + 280 counts @16:11:11.63 Forearm.reconfigure :tight @16:11:11.69 Forearm.seek 5 + 450 counts @16:11:11.91 SE.seek 7.16 Pucks below Top Plate @16:11:12.47 Forearm.reconfigure :loose @16:11:12.52 Forearm.seek :retract @16:11:13.43 Elbow.seek CC @16:11:15.02 Elbow.reconfigure :loose @16:11:15.12 Forearm.seek CC @16:11:16.07 Hand.close @16:11:16.75 CC.seek :open @16:11:21.50 Forearm.seek :retract @16:11:22.51 Elbow.reconfigure :tight @16:11:22.56 Elbow.seek 5 @16:11:24.35 Elbow.reconfigure :limp @16:11:24.40 Forearm.seek 5 @16:11:25.35 Elbow.hold @16:11:25.42 Elbow.reconfigure :tight @16:11:25.47 Hand.open @16:11:26.24 Forearm.force -20 @16:11:26.30 Forearm.seek 5 + 311 counts @16:11:26.60 Forearm.reconfigure :tight @16:11:26.65 Forearm.seek 5 + 450 counts @16:11:27.25 SE.seek :down @16:11:28.39 Forearm.reconfigure :loose @16:11:28.44 Forearm.seek :retract @16:11:29.46 SE.stop @16:11:29.50
Starting finalPurge @16:11:36.03 PSV.dial PBV @16:11:36.51 PS.seek 2.5ml @16:11:49.46
Waiting >20 seconds for psFlush to relinquish FlushPuck @16:12:02.87 PSV.dial :waste1 @16:12:03.63 PS.seek 0ml @16:12:29.98
Loading FlushPuck into Collection Clamp from Processing Clamp @16:12:30.16 Elbow.seek PC @16:12:32.66 Elbow.reconfigure :loose @16:12:32.76 Forearm.seek PC @16:12:33.71 Hand.close @16:12:34.37 PC.seek :open @16:12:39.09 Forearm.seek :retract @16:12:40.04 Elbow.reconfigure :tight @16:12:40.09 Elbow.seek CC @16:12:43.72 Elbow.reconfigure :loose @16:12:43.82 Forearm.reconfigure :tight @16:12:43.87 Forearm.seek CC @16:12:45.16 CC.seek :closed @16:12:49.70 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.42 @16:12:49.76 Hand.open @16:12:50.81 Forearm.reconfigure :loose @16:12:50.93 Forearm.seek :retract @16:12:51.92 Flushing the Collection Valve::Manifold @16:12:52.04 CSV.dial CSR @16:12:52.48 Pulling 5ml of CSR.flush @16:12:52.49 CSR.select :flush @16:12:52.61 CS.seek 5ml @16:13:06.23 delay 5 seconds @16:13:11.25 CSR.select :air @16:13:11.43 CS.reconfigure :fast @16:13:11.47 CS.seek 7.5ml @16:13:18.32 CSV.dial :waste @16:13:19.62 CS.reconfigure :default @16:13:19.67 CS.seek 0ml @16:13:39.64 Flushing lower manifold's waste1 port @16:13:39.76 CSV.dial :flush @16:13:40.22 CS.reconfigure :fast @16:13:40.27 CS.seek 5ml @16:13:53.17 delay 5 seconds @16:13:58.19 CSV.dial :air @16:13:58.70 CS.seek 7ml @16:14:04.23 CSV.dial CTV @16:14:04.25
CTV.dial CSV @16:14:05.65 CBV.dial :waste1 @16:14:06.42 CS.seek 0ml @16:14:23.04 Flushing lower manifold's waste2 port @16:14:23.16 CSV.dial :flush @16:14:23.92 CS.seek 5ml @16:14:36.85 delay 5 seconds @16:14:41.87 CSV.dial :air @16:14:42.51 CS.seek 7ml @16:14:48.07 CSV.dial CTV @16:14:48.10
CTV.dial CSV @16:14:48.74 CBV.dial :waste2 @16:14:49.81 CS.seek 0ml @16:15:06.42 Flushing lower manifold's waste2b port @16:15:06.53 CSV.dial :flush @16:15:07.38 CS.seek 5ml @16:15:20.10 delay 5 seconds @16:15:25.12 CSV.dial :air @16:15:25.62 CS.seek 7ml @16:15:31.17 CSV.dial CTV @16:15:31.21
CTV.dial CSV @16:15:31.63 CBV.dial :waste2b @16:15:32.69 CS.seek 0ml @16:15:49.72 Disinfecting external Intake with kill @16:15:49.86 CSV.dial CSR @16:15:50.32 Pulling 8ml of CSR.kill @16:15:50.33 CSR.select :kill @16:15:50.45 CS.seek 8ml @16:16:10.45 delay 5 seconds @16:16:15.46 CSR.select :air @16:16:15.59 CS.seek 10ml @16:16:21.23 CSV.dial CBV @16:16:21.25
CBV.dial CSV @16:16:22.44 CTV.dial :intake @16:16:23.19 Intake.select :open @16:16:23.31 CS.seek 2ml @16:16:42.29 Intake.select :close @16:16:42.39 CTV.dial :air @16:16:43.18 CS.reconfigure :default @16:16:43.23 CS.seek 0ml @16:16:49.19 CSV.dial CSR @16:16:50.21 Pulling 5ml of CSR.flush @16:16:50.22 CSR.select :flush @16:16:50.34 CS.seek 5ml @16:17:04.16 delay 5 seconds @16:17:09.18 CSR.select :air @16:17:09.30 CS.seek 10ml @16:17:22.91 CSV.dial CBV @16:17:24.06 CS.seek 0ml @16:17:50.54 Garaging FlushPuck in Collection Clamp @16:17:50.61 Elbow.reconfigure :tight @16:17:50.66 Elbow.seek CC @16:17:50.94 Elbow.reconfigure :loose @16:17:51.03 Forearm.seek CC @16:17:52.08 Hand.close @16:17:52.74 CC.seek :open @16:17:57.37 Forearm.seek :retract @16:17:58.40 Elbow.reconfigure :tight @16:17:58.44 Elbow.seek :garage @16:18:01.40 Elbow.reconfigure :loose @16:18:01.51 Forearm.reconfigure :tight @16:18:01.55 Forearm.seek :garage @16:18:02.53 Hand.open @16:18:03.17 Forearm.reconfigure :loose @16:18:03.22 Forearm.seek :retract @16:18:04.19 Completed [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] begun 13:35:29 @16:18:04.24 Asleep after completing DA_WCR_120823 assays @16:18:04.26 GoodNews.email "{1 => 33,\n 2 => 33,\n 3 => 31,\n 4 => 33,\n 5 => 8,\n 6 => 25,\n 7 => 31}\nCTD data unavailable\nCan@16:14:40, 30% flow, 17.0C, 32% humidity, 15.1psia, 13.656V, 0.453A, 179.277Ah, 6.19W\nSampled 625.0ml",Subject: "Asleep after completing DA_WCR_120823 assays" @16:18:08.97 upsync + ESPwhich DA_WCR_120823b.rb + fn=DA_WCR_120823b.rb + IFS=: + set /home/esp/esp2/mission . + fullPath=/home/esp/esp2/mission/DA_WCR_120823b.rb + [ -f /home/esp/esp2/mission/DA_WCR_120823b.rb ] + realpath /home/esp/esp2/mission/DA_WCR_120823b.rb + return 0 + scriptPath=/home/esp/esp2local/mission/DA_WCR_120823b.rb + exec ruby /home/esp/esp2/lib/esp.rb /home/esp/esp2local/mission/DA_WCR_120823b.rb @22:15:39.94UTC08-Dec-23
ESP2 v3.42 I2C Gateway v3.20 for core bus @22:15:42.52 Configuration for short puck jake 1/11/21 by brent@mbari.org /home/esp/esp2/type/shallow/jake/configure.rb @22:15:46.48 Polling Can every 10:00 @22:15:46.61 Cleanly exited at 20:30:55UTC08-Dec-23 @22:15:46.65 Loading /home/esp/esp2local/mission/DA_WCR_120823b.rb mission @22:15:46.71 Preparing to sleep until + 00:00:05 @22:15:46.72 GoodNews.email "Starting DA_WCR_120823b w/pucks in tube 6\n{1 => 33,\n 2 => 33,\n 3 => 31,\n 4 => 33,\n 5 => 8,\n 6 => 25,\n 7 => 31}\nCTD data unavailable\nCan@22:15:46, 30% flow, 17.0C, 33% humidity, 15.2psia, 13.720V, 0.272A, 180.744Ah, 3.74W",Subject: "DA_WCR_120823b begun" @22:15:46.76
Asleep til + 00:00:05 @22:15:46.80 Continuing at 22:15 @22:15:47.05 Gate.power :core,:OFF @22:15:47.06 Gate.power :raw,:OFF @22:15:47.07 Shutdown @22:15:50.17 GoodNews.email "Then, in tube 6: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_120823b.rb:14\n{1 => 33,\n 2 => 33,\n 3 => 31,\n 4 => 33,\n 5 => 8,\n 6 => 25,\n 7 => 31}\nCTD data unavailable\nCan@22:15:46, 30% flow, 17.0C, 33% humidity, 15.2psia, 13.720V, 0.272A, 180.744Ah, 3.74W",Subject: "Asleep until 22:15" @22:15:51.56 upsync @22:15:51.71
Powering core bus @22:15:54.71 upsync @22:15:57.08
Gate.power :raw,:ON @22:15:58.00 CS.configure :default @22:15:58.05 PS.configure :default @22:15:58.10 SS.configure :default @22:15:58.14 CC.configure :default @22:15:58.19 PC.configure :default @22:15:58.26 Forearm.configure :loose @22:15:58.31 Elbow.configure :loose @22:15:58.38 SC.configure :loose @22:15:58.42 SE.configure :default @22:15:58.47 CH.configure :default @22:15:58.51 PH.configure :default @22:15:58.55 All dwarf controllers running firmware version 3.85 @22:15:58.66 Valve.reset @22:15:59.30 Intake.select :close @22:15:59.33 Exhaust.select :close @22:15:59.37 CTV.dial :air @22:15:59.57 PSV.dial :air @22:15:59.61 CSV.dial :waste1 @22:15:59.71
Forearm.home.seek :retract @22:16:00.11 SSV.dial :spit,avoiding: :exhaust @22:16:00.52 Hand.close @22:16:00.56
Elbow.reconfigure :loose @22:16:00.65 Elbow.jog 1500 @22:16:00.81 PS.home.seek 0ml @22:16:00.99 CS.reconfigure :fast @22:16:01.03 SS.home.seek 0ml @22:16:01.12 CS.home.seek 0ml @22:16:01.17
Elbow.reconfigure :loose @22:16:01.29 Elbow.home.seek :clear @22:16:04.60 SE.home.seek :down @22:16:05.20 SC.reconfigure :tight @22:16:05.25 SC.home.seek :initial @22:16:09.27 PS.stop @22:16:27.33
SC.configure :limp @22:16:27.38 Forearm.seek :retract @22:16:27.44 Elbow.seek :hammer @22:16:28.47 Forearm.reconfigure :tight @22:16:28.52 Forearm.seek retracted @22:16:28.89 PC.home.seek :open @22:16:28.91
CC.home.seek :open @22:16:32.40 Phase [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] @22:16:32.41 Notice.email "At 22:16:32 began in tube 6: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_120823b.rb:14\n{1 => 33,\n 2 => 33,\n 3 => 31,\n 4 => 33,\n 5 => 8,\n 6 => 25,\n 7 => 31}\nCTD data unavailable\nCan@22:16:32, 30% flow, 17.1C, 33% humidity, 15.2psia, 13.715V, 0.272A, 180.749Ah, 3.73W",Subject: "Started DA_WCR_120823b phase initialPurge; da [1000, 1000] #da and wsr are run simultanelously" @22:16:32.44
Starting initialPurge @22:16:32.45 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @22:16:32.51 Hand.open @22:16:33.26 Elbow.reconfigure :tight @22:16:33.31 Elbow.seek FlushPuck::Garage @22:16:35.20 Elbow.reconfigure :loose @22:16:35.32 Forearm.reconfigure :loose @22:16:35.36 Forearm.seek :garage @22:16:36.32 Hand.close @22:16:36.78 upsync @22:16:37.02
Forearm.seek :retract @22:16:37.98 Elbow.reconfigure :tight @22:16:38.03 Elbow.seek CC @22:16:41.00 Elbow.reconfigure :loose @22:16:41.11 Forearm.reconfigure :tight @22:16:41.15 Forearm.seek CC @22:16:42.42 CC.seek :closed @22:16:46.95 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.43 @22:16:47.02 Hand.open @22:16:47.71 Forearm.reconfigure :loose @22:16:47.75 Forearm.seek :retract @22:16:48.76 Flushing the Collection Valve::Manifold @22:16:48.88 CSV.dial CSR @22:16:50.18 Pulling 5ml of CSR.flush CSR.select :flush @22:16:50.31 CS.reconfigure :default @22:16:50.35 CS.seek 5ml @22:17:04.60 delay 5 seconds @22:17:09.61 CSR.select :air @22:17:09.79 CS.reconfigure :fast @22:17:09.83 CS.seek 7.501ml @22:17:16.60 CSV.dial :waste @22:17:17.90 CS.reconfigure :default @22:17:17.94 CS.seek 0ml @22:17:37.91 Purging external Intake @22:17:38.03 CSV.dial :flush @22:17:38.49 CS.reconfigure :fast @22:17:38.54 CS.seek 10ml @22:18:03.03 delay 5 seconds @22:18:08.05 CSV.dial CBV @22:18:08.08
CBV.dial CSV @22:18:08.84 CTV.dial :intake @22:18:09.64 Intake.select :open @22:18:09.75 CS.seek 0ml @22:18:33.47 Intake.select :close @22:18:33.57 CTV.dial :air @22:18:34.35 Flushing upper manifold @22:18:34.47 CSV.dial :flush @22:18:35.21 CS.seek 5ml @22:18:47.94 delay 5 seconds @22:18:52.96 CSV.dial :air @22:18:53.47 CS.seek 7ml @22:18:58.97 CSV.dial CBV @22:18:58.99
CBV.dial CSV @22:19:00.05 CTV.dial :air @22:19:00.07 CS.seek 0ml @22:19:16.94 delay 7 seconds @22:19:23.96 CS.seek 3ml @22:19:31.97 CSV.dial :waste @22:19:32.43 CS.seek 0ml @22:19:40.23 CSV.dial :flush @22:19:40.68 CS.seek 3ml @22:19:48.81 delay 5 seconds @22:19:53.83 CSV.dial :air @22:19:54.34 CS.seek 5ml @22:20:00.06 CSV.dial :air @22:20:00.24 CS.seek 0ml @22:20:12.50 delay 7 seconds @22:20:19.53 CS.seek 3ml @22:20:27.81 CSV.dial :waste @22:20:28.55 CS.seek 0ml @22:20:36.28 Garaging FlushPuck in Collection Clamp @22:20:36.35 Elbow.reconfigure :tight @22:20:36.40 Elbow.seek CC @22:20:36.72 Elbow.reconfigure :loose @22:20:36.82 Forearm.seek CC @22:20:37.81 Hand.close @22:20:38.40 CC.seek :open @22:20:43.42 Forearm.seek :retract @22:20:44.47 Elbow.reconfigure :tight @22:20:44.52 Elbow.seek :garage @22:20:47.45 Elbow.reconfigure :loose @22:20:47.55 Forearm.reconfigure :tight @22:20:47.59 Forearm.seek :garage @22:20:48.58 Hand.open @22:20:49.20 Forearm.reconfigure :loose @22:20:49.25 Forearm.seek :retract @22:20:50.27 da sampling at most 1000ml, wcr at most 1000ml @22:20:50.31 DAfans.email "Started da in tube 6 at 22:20:50UTC08-Dec-23\n{1 => 33,\n 2 => 33,\n 3 => 31,\n 4 => 33,\n 5 => 8,\n 6 => 25,\n 7 => 31}\nCTD data unavailable\nCan@22:16:32, 30% flow, 17.1C, 33% humidity, 15.2psia, 13.715V, 0.272A, 180.749Ah, 3.73W",Subject: "da sampling at most 1000ml, wcr at most 1000ml" @22:20:50.33
Loading sh1 puck from tube 6 into Collection Clamp @22:20:50.60 SC.reconfigure :tight @22:20:50.66 SC.seek 6 @22:20:54.79 upsync @22:20:57.31
SC.configure :limp @22:20:57.54 Elbow.reconfigure :tight @22:20:57.59 Elbow.seek 6 @22:20:59.55 Forearm.reconfigure :tight @22:20:59.60 Forearm.seek 6 + 450 counts @22:21:00.60 SE.reconfigure :pick @22:21:00.66 SE.seek :beamBreak @22:21:02.21 SE.configure :default @22:21:02.37 Elbow.seek 6 @22:21:02.55 Forearm.seek 6 + 300 counts @22:21:02.85 Forearm.reconfigure :loose @22:21:02.89 Hand.close @22:21:03.53 SE.seek :down @22:21:03.74
Forearm.seek :retract @22:21:04.67 SE.stop @22:21:05.18
Elbow.seek CC @22:21:06.81 Elbow.reconfigure :loose @22:21:06.91 Forearm.reconfigure :tight @22:21:06.96 Forearm.seek CC @22:21:08.22 CC.seek :closed @22:21:12.75 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.53 @22:21:12.76 Hand.open @22:21:13.45 Forearm.reconfigure :loose @22:21:13.50 Forearm.seek :retract @22:21:14.50 collectSample 1000ml w/bubble point of 24psi @22:21:14.52 Priming 7ml Intake.select :close @22:21:14.53 Exhaust.select :close @22:21:14.55 SS.hold @22:21:14.76 Calibrating pressure and temperature sensors @22:21:14.79 CTV.dial :air @22:21:14.80 SSV.dial :spit,avoiding: :exhaust @22:21:15.00 SS.reconfigure :fast @22:21:15.05 SS.seek 5ml @22:21:17.93 SSV.dial CBV @22:21:17.95
CBV.dial SSV @22:21:19.31 delay 3.8 seconds @22:21:23.24 Sensor limits: Intake max 100.3psia; Outlet max 93.8psia @22:21:23.25 SSV.dial :spit @22:21:24.58 SS.seek 0ml @22:21:27.41 SSV.dial CBV @22:21:28.74 CTV.dial :intake @22:21:29.52 SSV.dial CBV @22:21:29.54
CBV.dial SSV @22:21:29.62 Allowing 40s for 7.0ml of remaining 7.0ml @22:21:29.63 Intake.select :open @22:21:31.80 Intake @14.6psia, vacuum from 9.1 to 12.1psi @22:21:31.81 SS.reconfigure :inhaling 2.20ml/s @22:21:31.86 SS.seek 7ml @22:21:38.80 Stroke completed -- waiting for pressure to equalize ... @22:21:38.81 SS.reconfigure :exhale @22:21:38.86 SS.hold @22:21:46.73 Intake.select :close @22:21:46.89 SSV.dial :exhaust @22:21:47.65 Exhaust.select :open @22:21:47.76 SS.seek 0ml @22:21:51.76 Exhaust.select :close @22:21:51.86 Sampling 1000.0ml @22:21:51.87 Intake.select :close @22:21:51.88 Exhaust.select :close @22:21:51.89 SS.reconfigure :default @22:21:51.94 SS.hold @22:21:52.14 CTV.dial :intake @22:21:52.22 SSV.dial CBV @22:21:52.24
CBV.dial SSV @22:21:53.01 Allowing 130s for 25.0ml of remaining 1000.0ml @22:21:53.02 Intake.select :open @22:21:55.19 Intake @15.3psia, vacuum from 9.8 to 12.8psi @22:21:55.20 SS.reconfigure :inhaling 2.20ml/s @22:21:55.25 SS.seek 25ml @22:22:03.94 Vacuum too high -- waiting up to 40 seconds @22:22:03.95 SS.reconfigure :exhale @22:22:04.00 SS.hold @22:22:09.96 Intake @17.3psia, vacuum from 10.0 to 13.0psi @22:22:09.98 SS.reconfigure :inhaling 1.76ml/s @22:22:10.02 SS.seek 25ml @22:22:13.45 Vacuum too high -- waiting up to 40 seconds @22:22:13.46 SS.reconfigure :exhale @22:22:13.56 SS.hold @22:22:16.15 Intake @18.1psia, vacuum from 10.0 to 13.0psi @22:22:16.17 SS.reconfigure :inhaling 1.41ml/s @22:22:16.21 SS.seek 25ml @22:22:19.76 Vacuum too high -- waiting up to 40 seconds @22:22:19.77 SS.reconfigure :exhale @22:22:19.82 SS.hold @22:22:21.98 Intake @17.9psia, vacuum from 10.0 to 13.0psi @22:22:22.00 SS.reconfigure :inhaling 1.13ml/s @22:22:22.05 SS.seek 25ml @22:22:26.33 Stroke completed -- waiting for pressure to equalize ... @22:22:26.34 SS.reconfigure :exhale @22:22:26.39 SS.hold @22:22:54.96 Intake.select :close @22:22:55.11 SSV.dial :exhaust @22:22:55.87 Exhaust.select :open @22:22:55.98 SS.seek 0ml @22:23:04.83 Exhaust.select :close @22:23:04.94 SSV.dial CBV @22:23:04.96
CBV.dial SSV @22:23:05.74 Allowing 130s for 25.0ml of remaining 975.0ml @22:23:05.75 Intake.select :open @22:23:07.92 Intake @16.0psia, vacuum from 10.0 to 13.0psi @22:23:07.93 SS.reconfigure :inhaling 1.13ml/s @22:23:07.98 SS.seek 25ml @22:23:32.93 Stroke completed -- waiting for pressure to equalize ... @22:23:32.94 SS.reconfigure :exhale @22:23:32.98 SS.hold @22:24:03.63 Intake.select :close @22:24:03.78 SSV.dial :exhaust @22:24:04.54 Exhaust.select :open @22:24:04.65 SS.seek 0ml @22:24:13.59 Exhaust.select :close @22:24:13.70 SSV.dial CBV @22:24:13.72
CBV.dial SSV @22:24:14.47 Allowing 130s for 25.0ml of remaining 950.0ml @22:24:14.49 Intake.select :open @22:24:16.68 Intake @16.1psia, vacuum from 10.0 to 13.0psi @22:24:16.69 SS.reconfigure :inhaling 1.13ml/s @22:24:16.74 SS.seek 25ml @22:24:41.60 Stroke completed -- waiting for pressure to equalize ... @22:24:41.61 SS.reconfigure :exhale @22:24:41.66 SS.hold @22:25:14.24 Intake.select :close @22:25:14.39 SSV.dial :exhaust @22:25:15.16 Exhaust.select :open @22:25:15.27 SS.seek 0ml @22:25:24.10 Exhaust.select :close @22:25:24.20 SSV.dial CBV @22:25:24.22
CBV.dial SSV @22:25:24.99 Allowing 130s for 25.0ml of remaining 925.0ml @22:25:25.00 Intake.select :open @22:25:27.17 Intake @16.3psia, vacuum from 10.0 to 13.0psi @22:25:27.18 SS.reconfigure :inhaling 1.13ml/s @22:25:27.23 SS.seek 25ml @22:25:52.00 Stroke completed -- waiting for pressure to equalize ... @22:25:52.01 SS.reconfigure :exhale @22:25:52.06 SS.hold @22:26:20.54 Intake.select :close @22:26:20.69 SSV.dial :exhaust @22:26:21.51 Exhaust.select :open @22:26:21.62 SS.seek 0ml @22:26:30.44 Exhaust.select :close @22:26:30.55 SSV.dial CBV @22:26:30.57
CBV.dial SSV @22:26:31.35 Allowing 130s for 25.0ml of remaining 900.0ml @22:26:31.36 Intake.select :open @22:26:33.52 Intake @16.3psia, vacuum from 10.0 to 13.0psi @22:26:33.53 SS.reconfigure :inhaling 1.13ml/s @22:26:33.58 SS.seek 25ml @22:26:58.30 Stroke completed -- waiting for pressure to equalize ... @22:26:58.31 SS.reconfigure :exhale @22:26:58.35 SS.hold @22:27:24.87 Intake.select :close @22:27:25.02 SSV.dial :exhaust @22:27:25.80 Exhaust.select :open @22:27:25.91 SS.seek 0ml @22:27:34.70 Exhaust.select :close @22:27:34.81 SSV.dial CBV @22:27:34.83
CBV.dial SSV @22:27:35.61 Allowing 130s for 25.0ml of remaining 875.0ml @22:27:35.62 Intake.select :open @22:27:37.79 Intake @16.3psia, vacuum from 10.0 to 13.0psi @22:27:37.80 SS.reconfigure :inhaling 1.13ml/s @22:27:37.85 SS.seek 25ml @22:28:01.11 Vacuum too high -- waiting up to 40 seconds @22:28:01.13 SS.reconfigure :exhale @22:28:01.18 SS.hold @22:28:07.34 Intake @17.1psia, vacuum from 10.0 to 13.0psi @22:28:07.36 SS.reconfigure :inhaling 0.91ml/s @22:28:07.40 SS.seek 25ml @22:28:09.65 Stroke completed -- waiting for pressure to equalize ... @22:28:09.66 SS.reconfigure :exhale @22:28:09.71 SS.hold @22:28:28.05 Intake.select :close @22:28:28.20 SSV.dial :exhaust @22:28:28.99 Exhaust.select :open @22:28:29.11 SS.seek 0ml @22:28:38.05 Exhaust.select :close @22:28:38.16 SSV.dial CBV @22:28:38.18
CBV.dial SSV @22:28:38.95 Allowing 130s for 25.0ml of remaining 850.0ml @22:28:38.96 Intake.select :open @22:28:41.13 Intake @16.3psia, vacuum from 10.0 to 13.0psi @22:28:41.15 SS.reconfigure :inhaling 0.91ml/s @22:28:41.20 SS.seek 25ml @22:29:10.64 Stroke completed -- waiting for pressure to equalize ... @22:29:10.65 SS.reconfigure :exhale @22:29:10.69 SS.hold @22:29:31.32 Intake.select :close @22:29:31.47 SSV.dial :exhaust @22:29:32.24 Exhaust.select :open @22:29:32.35 SS.seek 0ml @22:29:41.33 Exhaust.select :close @22:29:41.44 SSV.dial CBV @22:29:41.47
CBV.dial SSV @22:29:42.20 Allowing 130s for 25.0ml of remaining 825.0ml @22:29:42.21 Intake.select :open @22:29:44.38 Intake @16.3psia, vacuum from 10.0 to 13.0psi @22:29:44.39 SS.reconfigure :inhaling 0.91ml/s @22:29:44.44 SS.seek 25ml @22:30:13.71 Stroke completed -- waiting for pressure to equalize ... @22:30:13.72 SS.reconfigure :exhale @22:30:13.77 SS.hold @22:30:34.25 Intake.select :close @22:30:34.40 SSV.dial :exhaust @22:30:35.16 Exhaust.select :open @22:30:35.28 SS.seek 0ml @22:30:43.95 Exhaust.select :close @22:30:44.06 SSV.dial CBV @22:30:44.08
CBV.dial SSV @22:30:44.86 Allowing 130s for 25.0ml of remaining 800.0ml @22:30:44.87 Intake.select :open @22:30:47.04 Intake @16.4psia, vacuum from 10.0 to 13.0psi @22:30:47.05 SS.reconfigure :inhaling 0.91ml/s @22:30:47.10 SS.seek 25ml @22:31:16.82 Stroke completed -- waiting for pressure to equalize ... @22:31:16.83 SS.reconfigure :exhale @22:31:16.88 SS.hold @22:31:35.29 Intake.select :close @22:31:35.45 SSV.dial :exhaust @22:31:36.22 Exhaust.select :open @22:31:36.33 SS.seek 0ml @22:31:45.07 Exhaust.select :close @22:31:45.18 SSV.dial CBV @22:31:45.21
CBV.dial SSV @22:31:45.97 Allowing 130s for 25.0ml of remaining 775.0ml @22:31:45.98 Intake.select :open @22:31:48.14 Intake @16.3psia, vacuum from 10.0 to 13.0psi @22:31:48.15 SS.reconfigure :inhaling 0.91ml/s @22:31:48.20 SS.seek 25ml @22:32:17.95 Stroke completed -- waiting for pressure to equalize ... @22:32:17.96 SS.reconfigure :exhale @22:32:18.00 SS.hold @22:32:34.73 Intake.select :close @22:32:34.88 SSV.dial :exhaust @22:32:35.64 Exhaust.select :open @22:32:35.75 SS.seek 0ml @22:32:44.53 Exhaust.select :close @22:32:44.64 SSV.dial CBV @22:32:44.66
CBV.dial SSV @22:32:45.43 Allowing 130s for 25.0ml of remaining 750.0ml @22:32:45.44 Intake.select :open @22:32:47.61 Intake @16.5psia, vacuum from 10.0 to 13.0psi @22:32:47.62 SS.reconfigure :inhaling 0.91ml/s @22:32:47.67 SS.seek 25ml @22:33:17.41 Stroke completed -- waiting for pressure to equalize ... @22:33:17.42 SS.reconfigure :exhale @22:33:17.47 SS.hold @22:33:35.84 Intake.select :close @22:33:36.42 SSV.dial :exhaust @22:33:37.20 Exhaust.select :open @22:33:37.31 SS.seek 0ml @22:33:46.09 Exhaust.select :close @22:33:46.19 SSV.dial CBV @22:33:46.21
CBV.dial SSV @22:33:46.98 Allowing 130s for 25.0ml of remaining 725.0ml @22:33:47.00 Intake.select :open @22:33:49.16 Intake @16.4psia, vacuum from 10.0 to 13.0psi @22:33:49.18 SS.reconfigure :inhaling 0.91ml/s @22:33:49.22 SS.seek 25ml @22:34:18.94 Stroke completed -- waiting for pressure to equalize ... @22:34:18.95 SS.reconfigure :exhale @22:34:19.00 SS.hold @22:34:37.33 Intake.select :close @22:34:37.49 SSV.dial :exhaust @22:34:38.24 Exhaust.select :open @22:34:38.36 SS.seek 0ml @22:34:47.38 Exhaust.select :close @22:34:47.49 SSV.dial CBV @22:34:47.52
CBV.dial SSV @22:34:48.29 Allowing 130s for 25.0ml of remaining 700.0ml @22:34:48.30 Intake.select :open @22:34:50.47 Intake @16.4psia, vacuum from 10.0 to 13.0psi @22:34:50.48 SS.reconfigure :inhaling 0.91ml/s @22:34:50.96 SS.seek 25ml @22:35:20.18 Stroke completed -- waiting for pressure to equalize ... @22:35:20.19 SS.reconfigure :exhale @22:35:20.23 SS.hold @22:35:38.52 Intake.select :close @22:35:38.68 SSV.dial :exhaust @22:35:40.26 Exhaust.select :open @22:35:40.37 SS.seek 0ml @22:35:49.10 Exhaust.select :close @22:35:49.21 SSV.dial CBV @22:35:49.25
CBV.dial SSV @22:35:50.02 Allowing 130s for 25.0ml of remaining 675.0ml @22:35:50.03 Intake.select :open @22:35:52.20 Intake @16.5psia, vacuum from 10.0 to 13.0psi @22:35:52.21 SS.reconfigure :inhaling 0.91ml/s @22:35:52.26 SS.seek 25ml @22:36:21.97 Stroke completed -- waiting for pressure to equalize ... @22:36:21.98 SS.reconfigure :exhale @22:36:22.03 SS.hold @22:36:42.62 Intake.select :close @22:36:42.77 SSV.dial :exhaust @22:36:43.55 Exhaust.select :open @22:36:43.66 SS.seek 0ml @22:36:52.40 Exhaust.select :close @22:36:52.51 SSV.dial CBV @22:36:52.53
CBV.dial SSV @22:36:53.31 Allowing 130s for 25.0ml of remaining 650.0ml @22:36:53.32 Intake.select :open @22:36:55.48 Intake @16.2psia, vacuum from 10.0 to 13.0psi @22:36:55.50 SS.reconfigure :inhaling 0.91ml/s @22:36:55.54 SS.seek 25ml @22:37:25.25 Stroke completed -- waiting for pressure to equalize ... @22:37:25.26 SS.reconfigure :exhale @22:37:25.31 SS.hold @22:37:43.72 Intake.select :close @22:37:43.87 SSV.dial :exhaust @22:37:44.63 Exhaust.select :open @22:37:44.75 SS.seek 0ml @22:37:53.53 Exhaust.select :close @22:37:53.64 SSV.dial CBV @22:37:53.68
CBV.dial SSV @22:37:54.43 Allowing 130s for 25.0ml of remaining 625.0ml @22:37:54.44 Intake.select :open @22:37:56.60 Intake @16.5psia, vacuum from 10.0 to 13.0psi @22:37:56.61 SS.reconfigure :inhaling 0.91ml/s @22:37:56.66 SS.seek 25ml @22:38:26.33 Stroke completed -- waiting for pressure to equalize ... @22:38:26.34 SS.reconfigure :exhale @22:38:26.39 SS.hold @22:38:46.75 Intake.select :close @22:38:46.90 SSV.dial :exhaust @22:38:47.67 Exhaust.select :open @22:38:47.78 SS.seek 0ml @22:38:56.53 Exhaust.select :close @22:38:56.64 SSV.dial CBV @22:38:56.66
CBV.dial SSV @22:38:57.44 Allowing 130s for 25.0ml of remaining 600.0ml @22:38:57.45 Intake.select :open @22:38:59.62 Intake @16.4psia, vacuum from 10.0 to 13.0psi @22:38:59.63 SS.reconfigure :inhaling 0.91ml/s @22:38:59.68 SS.seek 25ml @22:39:29.78 Stroke completed -- waiting for pressure to equalize ... @22:39:29.79 SS.reconfigure :exhale @22:39:29.84 SS.hold @22:39:50.55 Intake.select :close @22:39:50.70 SSV.dial :exhaust @22:39:51.48 Exhaust.select :open @22:39:51.60 SS.seek 0ml @22:40:00.35 Exhaust.select :close @22:40:00.45 SSV.dial CBV @22:40:00.47
CBV.dial SSV @22:40:01.25 Allowing 130s for 25.0ml of remaining 575.0ml @22:40:01.26 Intake.select :open @22:40:03.43 Intake @16.3psia, vacuum from 10.0 to 13.0psi @22:40:03.44 SS.reconfigure :inhaling 0.91ml/s @22:40:03.49 SS.seek 25ml @22:40:33.00 Stroke completed -- waiting for pressure to equalize ... @22:40:33.01 SS.reconfigure :exhale @22:40:33.06 SS.hold @22:40:55.53 Intake.select :close @22:40:55.68 SSV.dial :exhaust @22:40:56.44 Exhaust.select :open @22:40:56.55 SS.seek 0ml @22:41:05.25 Exhaust.select :close @22:41:05.36 SSV.dial CBV @22:41:05.38
CBV.dial SSV @22:41:06.16 Allowing 130s for 25.0ml of remaining 550.0ml @22:41:06.17 Intake.select :open @22:41:08.34 Intake @16.4psia, vacuum from 10.0 to 13.0psi @22:41:08.35 SS.reconfigure :inhaling 0.91ml/s @22:41:08.40 SS.seek 25ml @22:41:38.17 Stroke completed -- waiting for pressure to equalize ... @22:41:38.18 SS.reconfigure :exhale @22:41:38.23 SS.hold @22:42:02.61 Intake.select :close @22:42:02.76 SSV.dial :exhaust @22:42:03.52 Exhaust.select :open @22:42:03.63 SS.seek 0ml @22:42:12.36 Exhaust.select :close @22:42:12.47 SSV.dial CBV @22:42:12.49
CBV.dial SSV @22:42:13.27 Allowing 130s for 25.0ml of remaining 525.0ml @22:42:13.28 Intake.select :open @22:42:15.45 Intake @16.4psia, vacuum from 10.0 to 13.0psi @22:42:15.46 SS.reconfigure :inhaling 0.91ml/s @22:42:15.51 SS.seek 25ml @22:42:42.07 Vacuum too high -- waiting up to 40 seconds @22:42:42.08 SS.reconfigure :exhale @22:42:42.13 SS.hold @22:42:53.38 Intake @17.6psia, vacuum from 10.0 to 13.0psi @22:42:53.40 SS.reconfigure :inhaling 0.73ml/s @22:42:53.45 SS.seek 25ml @22:42:57.09 Stroke completed -- waiting for pressure to equalize ... @22:42:57.10 SS.reconfigure :exhale @22:42:57.15 SS.hold @22:43:10.18 Intake.select :close @22:43:10.33 SSV.dial :exhaust @22:43:11.09 Exhaust.select :open @22:43:11.21 SS.seek 0ml @22:43:19.98 Exhaust.select :close @22:43:20.09 SSV.dial CBV @22:43:20.11
CBV.dial SSV @22:43:20.89 Allowing 130s for 25.0ml of remaining 500.0ml @22:43:20.90 Intake.select :open @22:43:23.07 Intake @16.4psia, vacuum from 10.0 to 13.0psi @22:43:23.08 SS.reconfigure :inhaling 0.73ml/s @22:43:23.13 SS.seek 25ml @22:43:59.35 Stroke completed -- waiting for pressure to equalize ... @22:43:59.37 SS.reconfigure :exhale @22:43:59.41 SS.hold @22:44:19.82 Intake.select :close @22:44:19.97 SSV.dial :exhaust @22:44:20.75 Exhaust.select :open @22:44:20.87 SS.seek 0ml @22:44:29.68 Exhaust.select :close @22:44:29.79 SSV.dial CBV @22:44:29.82
CBV.dial SSV @22:44:30.59 Allowing 130s for 25.0ml of remaining 475.0ml @22:44:30.60 Intake.select :open @22:44:32.77 Intake @16.4psia, vacuum from 10.0 to 13.0psi @22:44:32.78 SS.reconfigure :inhaling 0.73ml/s @22:44:32.83 SS.seek 25ml @22:45:09.09 Stroke completed -- waiting for pressure to equalize ... @22:45:09.10 SS.reconfigure :exhale @22:45:09.14 SS.hold @22:45:31.54 Intake.select :close @22:45:31.69 SSV.dial :exhaust @22:45:32.45 Exhaust.select :open @22:45:32.56 SS.seek 0ml @22:45:41.29 Exhaust.select :close @22:45:41.40 SSV.dial CBV @22:45:41.44
CBV.dial SSV @22:45:42.20 Allowing 130s for 25.0ml of remaining 450.0ml @22:45:42.21 Intake.select :open @22:45:44.38 Intake @16.5psia, vacuum from 10.0 to 13.0psi @22:45:44.39 SS.reconfigure :inhaling 0.73ml/s @22:45:44.44 SS.seek 25ml @22:46:20.82 Stroke completed -- waiting for pressure to equalize ... @22:46:20.83 SS.reconfigure :exhale @22:46:20.88 SS.hold @22:46:43.77 Intake.select :close @22:46:43.92 SSV.dial :exhaust @22:46:44.68 Exhaust.select :open @22:46:44.80 SS.seek 0ml @22:46:53.59 Exhaust.select :close @22:46:53.69 SSV.dial CBV @22:46:53.71
CBV.dial SSV @22:46:54.48 Allowing 130s for 25.0ml of remaining 425.0ml @22:46:54.49 Intake.select :open @22:46:56.66 Intake @16.6psia, vacuum from 10.0 to 13.0psi @22:46:56.68 SS.reconfigure :inhaling 0.73ml/s @22:46:56.73 SS.seek 25ml @22:47:29.12 Vacuum too high -- waiting up to 40 seconds @22:47:29.14 SS.reconfigure :exhale @22:47:29.19 SS.hold @22:47:39.53 Intake @18.1psia, vacuum from 10.0 to 13.0psi @22:47:39.55 SS.reconfigure :inhaling 0.59ml/s @22:47:39.60 SS.seek 25ml @22:47:44.45 Stroke completed -- waiting for pressure to equalize ... @22:47:44.46 SS.reconfigure :exhale @22:47:44.51 SS.hold @22:47:58.89 Intake.select :close @22:47:59.04 SSV.dial :exhaust @22:47:59.81 Exhaust.select :open @22:47:59.92 SS.seek 0ml @22:48:08.69 Exhaust.select :close @22:48:08.80 SSV.dial CBV @22:48:08.84
CBV.dial SSV @22:48:09.59 Allowing 130s for 25.0ml of remaining 400.0ml @22:48:09.60 Intake.select :open @22:48:11.76 Intake @16.5psia, vacuum from 10.0 to 13.0psi @22:48:11.77 SS.reconfigure :inhaling 0.59ml/s @22:48:11.82 SS.seek 25ml @22:48:57.15 Stroke completed -- waiting for pressure to equalize ... @22:48:57.16 SS.reconfigure :exhale @22:48:57.20 SS.hold @22:49:18.65 Intake.select :close @22:49:18.81 SSV.dial :exhaust @22:49:19.59 Exhaust.select :open @22:49:19.70 SS.seek 0ml @22:49:28.68 Exhaust.select :close @22:49:28.79 SSV.dial CBV @22:49:28.81
CBV.dial SSV @22:49:29.59 Allowing 130s for 25.0ml of remaining 375.0ml @22:49:29.60 Intake.select :open @22:49:31.76 Intake @16.5psia, vacuum from 10.0 to 13.0psi @22:49:31.77 SS.reconfigure :inhaling 0.59ml/s @22:49:31.82 SS.seek 25ml @22:50:16.21 Stroke completed -- waiting for pressure to equalize ... @22:50:16.22 SS.reconfigure :exhale @22:50:16.26 SS.hold @22:50:38.73 Intake.select :close @22:50:38.88 SSV.dial :exhaust @22:50:39.65 Exhaust.select :open @22:50:39.76 SS.seek 0ml @22:50:48.75 Exhaust.select :close @22:50:48.86 SSV.dial CBV @22:50:48.89
CBV.dial SSV @22:50:49.65 Allowing 130s for 25.0ml of remaining 350.0ml @22:50:49.66 Intake.select :open @22:50:51.82 Intake @16.7psia, vacuum from 10.0 to 13.0psi @22:50:51.83 SS.reconfigure :inhaling 0.59ml/s @22:50:51.88 SS.seek 25ml @22:51:34.21 Vacuum too high -- waiting up to 40 seconds @22:51:34.23 SS.reconfigure :exhale @22:51:34.28 SS.hold @22:51:45.62 Intake @19.5psia, vacuum from 10.0 to 13.0psi @22:51:45.63 SS.reconfigure :inhaling 0.47ml/s @22:51:45.68 SS.seek 25ml @22:51:48.80 Stroke completed -- waiting for pressure to equalize ... @22:51:48.81 SS.reconfigure :exhale @22:51:48.86 SS.hold @22:52:05.48 Intake.select :close @22:52:05.64 SSV.dial :exhaust @22:52:06.40 Exhaust.select :open @22:52:06.52 SS.seek 0ml @22:52:15.28 Exhaust.select :close @22:52:15.39 SSV.dial CBV @22:52:15.43
CBV.dial SSV @22:52:16.18 Allowing 130s for 25.0ml of remaining 325.0ml @22:52:16.19 Intake.select :open @22:52:18.35 Intake @16.3psia, vacuum from 10.0 to 13.0psi @22:52:18.36 SS.reconfigure :inhaling 0.47ml/s @22:52:18.41 SS.seek 25ml @22:53:13.70 Stroke completed -- waiting for pressure to equalize ... @22:53:13.71 SS.reconfigure :exhale @22:53:13.76 SS.hold @22:53:34.14 Intake.select :close @22:53:34.29 SSV.dial :exhaust @22:53:35.07 Exhaust.select :open @22:53:35.19 SS.seek 0ml @22:53:44.53 Exhaust.select :close @22:53:44.64 SSV.dial CBV @22:53:44.69
CBV.dial SSV @22:53:45.43 Allowing 130s for 25.0ml of remaining 300.0ml @22:53:45.44 Intake.select :open @22:53:47.60 Intake @16.6psia, vacuum from 10.0 to 13.0psi @22:53:47.61 SS.reconfigure :inhaling 0.47ml/s @22:53:47.66 SS.seek 25ml @22:54:42.55 Stroke completed -- waiting for pressure to equalize ... @22:54:42.56 SS.reconfigure :exhale @22:54:42.60 SS.hold @22:55:05.03 Intake.select :close @22:55:05.18 SSV.dial :exhaust @22:55:05.94 Exhaust.select :open @22:55:06.05 SS.seek 0ml @22:55:15.03 Exhaust.select :close @22:55:15.14 SSV.dial CBV @22:55:15.16
CBV.dial SSV @22:55:16.36 Allowing 130s for 25.0ml of remaining 275.0ml @22:55:16.37 Intake.select :open @22:55:18.54 Intake @16.8psia, vacuum from 10.0 to 13.0psi @22:55:18.55 SS.reconfigure :inhaling 0.47ml/s @22:55:18.60 SS.seek 25ml @22:56:13.43 Stroke completed -- waiting for pressure to equalize ... @22:56:13.45 SS.reconfigure :exhale @22:56:13.49 SS.hold @22:56:40.06 Intake.select :close @22:56:40.21 SSV.dial :exhaust @22:56:40.97 Exhaust.select :open @22:56:41.09 SS.seek 0ml @22:56:50.09 Exhaust.select :close @22:56:50.20 SSV.dial CBV @22:56:50.58
CBV.dial SSV @22:56:51.42 Allowing 130s for 25.0ml of remaining 250.0ml @22:56:51.43 Intake.select :open @22:56:53.60 Intake @16.6psia, vacuum from 10.0 to 13.0psi @22:56:53.61 SS.reconfigure :inhaling 0.47ml/s @22:56:53.66 SS.seek 25ml @22:57:48.90 Stroke completed -- waiting for pressure to equalize ... @22:57:48.91 SS.reconfigure :exhale @22:57:48.96 SS.hold @22:58:15.53 Intake.select :close @22:58:15.68 SSV.dial :exhaust @22:58:16.47 Exhaust.select :open @22:58:16.59 SS.seek 0ml @22:58:25.32 Exhaust.select :close @22:58:25.79 SSV.dial CBV @22:58:25.88
CBV.dial SSV @22:58:26.65 Allowing 130s for 25.0ml of remaining 225.0ml @22:58:26.67 Intake.select :open @22:58:28.83 Intake @16.8psia, vacuum from 10.0 to 13.0psi @22:58:28.85 SS.reconfigure :inhaling 0.47ml/s @22:58:28.90 SS.seek 25ml @22:59:24.23 Stroke completed -- waiting for pressure to equalize ... @22:59:24.24 SS.reconfigure :exhale @22:59:24.28 SS.hold @22:59:52.86 Intake.select :close @22:59:53.01 SSV.dial :exhaust @22:59:53.78 Exhaust.select :open @22:59:53.89 SS.seek 0ml @23:00:03.34 Exhaust.select :close @23:00:03.44 SSV.dial CBV @23:00:03.48
CBV.dial SSV @23:00:04.23 Allowing 130s for 25.0ml of remaining 200.0ml @23:00:04.24 Intake.select :open @23:00:06.41 Intake @16.7psia, vacuum from 10.0 to 13.0psi @23:00:06.42 SS.reconfigure :inhaling 0.47ml/s @23:00:06.47 SS.seek 25ml @23:01:01.38 Stroke completed -- waiting for pressure to equalize ... @23:01:01.39 SS.reconfigure :exhale @23:01:01.44 SS.hold @23:01:34.56 Intake.select :close @23:01:34.72 SSV.dial :exhaust @23:01:35.49 Exhaust.select :open @23:01:35.60 SS.seek 0ml @23:01:44.38 Exhaust.select :close @23:01:44.50 SSV.dial CBV @23:01:44.54
CBV.dial SSV @23:01:45.28 Allowing 130s for 25.0ml of remaining 175.0ml @23:01:45.29 Intake.select :open @23:01:47.45 Intake @16.8psia, vacuum from 10.0 to 13.0psi @23:01:47.46 SS.reconfigure :inhaling 0.47ml/s @23:01:47.51 SS.seek 25ml @23:02:43.15 Stroke completed -- waiting for pressure to equalize ... @23:02:43.16 SS.reconfigure :exhale @23:02:43.20 SS.hold @23:03:16.29 Intake.select :close @23:03:16.44 SSV.dial :exhaust @23:03:17.22 Exhaust.select :open @23:03:17.33 SS.seek 0ml @23:03:26.29 Exhaust.select :close @23:03:26.39 SSV.dial CBV @23:03:26.42
CBV.dial SSV @23:03:27.19 Allowing 130s for 25.0ml of remaining 150.0ml @23:03:27.21 Intake.select :open @23:03:29.37 Intake @16.9psia, vacuum from 10.0 to 13.0psi @23:03:29.39 SS.reconfigure :inhaling 0.47ml/s @23:03:29.44 SS.seek 25ml @23:04:16.76 Vacuum too high -- waiting up to 40 seconds @23:04:16.78 SS.reconfigure :exhale @23:04:16.83 SS.hold @23:04:33.22 Intake @19.2psia, vacuum from 10.0 to 13.0psi @23:04:33.23 SS.reconfigure :inhaling 0.38ml/s @23:04:33.71 SS.seek 25ml @23:04:44.29 Vacuum too high -- waiting up to 40 seconds @23:04:44.31 SS.reconfigure :exhale @23:04:44.36 SS.hold @23:04:49.31 Intake @18.4psia, vacuum from 10.0 to 13.0psi @23:04:49.32 SS.reconfigure :inhaling 0.31ml/s @23:04:49.37 SS.seek 25ml @23:04:49.95 Stroke completed -- waiting for pressure to equalize ... @23:04:49.97 SS.reconfigure :exhale @23:04:50.01 SS.hold @23:05:08.41 Intake.select :close @23:05:08.56 SSV.dial :exhaust @23:05:09.32 Exhaust.select :open @23:05:09.43 SS.seek 0ml @23:05:18.28 Exhaust.select :close @23:05:18.39 SSV.dial CBV @23:05:18.42
CBV.dial SSV @23:05:19.18 Allowing 130s for 25.0ml of remaining 125.0ml @23:05:19.19 Intake.select :open @23:05:21.36 Intake @16.8psia, vacuum from 10.0 to 13.0psi @23:05:21.37 SS.reconfigure :inhaling 0.31ml/s @23:05:21.42 SS.seek 25ml @23:06:47.55 Stroke completed -- waiting for pressure to equalize ... @23:06:47.56 SS.reconfigure :exhale @23:06:47.60 SS.hold @23:07:14.12 Intake.select :close @23:07:14.27 SSV.dial :exhaust @23:07:15.04 Exhaust.select :open @23:07:15.16 SS.seek 0ml @23:07:24.19 Exhaust.select :close @23:07:24.30 SSV.dial CBV @23:07:24.32
CBV.dial SSV @23:07:25.10 Allowing 130s for 25.0ml of remaining 100.0ml @23:07:25.11 Intake.select :open @23:07:27.27 Intake @16.7psia, vacuum from 10.0 to 13.0psi @23:07:27.28 SS.reconfigure :inhaling 0.31ml/s @23:07:27.33 SS.seek 25ml @23:08:53.98 Stroke completed -- waiting for pressure to equalize ... @23:08:53.99 SS.reconfigure :exhale @23:08:54.04 SS.hold @23:09:25.11 Intake.select :close @23:09:25.26 SSV.dial :exhaust @23:09:26.03 Exhaust.select :open @23:09:26.15 SS.seek 0ml @23:09:34.94 Exhaust.select :close @23:09:35.04 SSV.dial CBV @23:09:35.06
CBV.dial SSV @23:09:35.86 Allowing 130s for 25.0ml of remaining 75.0ml @23:09:35.87 Intake.select :open @23:09:38.04 Intake @16.7psia, vacuum from 10.0 to 13.0psi @23:09:38.05 SS.reconfigure :inhaling 0.31ml/s @23:09:38.10 SS.seek 25ml @23:11:05.78 Stroke completed -- waiting for pressure to equalize ... @23:11:05.79 SS.reconfigure :exhale @23:11:05.83 SS.hold @23:11:36.82 Intake.select :close @23:11:36.97 SSV.dial :exhaust @23:11:37.75 Exhaust.select :open @23:11:37.86 SS.seek 0ml @23:11:46.87 Exhaust.select :close @23:11:46.98 SSV.dial CBV @23:11:47.00
CBV.dial SSV @23:11:47.78 Allowing 130s for 25.0ml of remaining 50.0ml @23:11:47.79 Intake.select :open @23:11:49.96 Intake @16.9psia, vacuum from 10.0 to 13.0psi @23:11:49.97 SS.reconfigure :inhaling 0.31ml/s @23:11:50.02 SS.seek 25ml @23:13:17.51 Stroke completed -- waiting for pressure to equalize ... @23:13:17.52 SS.reconfigure :exhale @23:13:17.56 SS.hold @23:13:54.32 Intake.select :close @23:13:54.47 SSV.dial :exhaust @23:13:55.23 Exhaust.select :open @23:13:55.34 SS.seek 0ml @23:14:04.37 Exhaust.select :close @23:14:04.47 SSV.dial CBV @23:14:04.49
CBV.dial SSV @23:14:05.26 Allowing 130s for 25.0ml of remaining 25.0ml @23:14:05.27 Intake.select :open @23:14:07.44 Intake @16.6psia, vacuum from 10.0 to 13.0psi @23:14:07.45 SS.reconfigure :inhaling 0.31ml/s @23:14:07.50 SS.seek 24.99ml @23:15:34.52 Stroke completed -- waiting for pressure to equalize ... @23:15:34.53 SS.reconfigure :exhale @23:15:34.58 SS.hold @23:16:09.20 Intake.select :close @23:16:09.36 SSV.dial :exhaust @23:16:10.12 Exhaust.select :open @23:16:10.23 SS.seek 0ml @23:16:19.07 Exhaust.select :close @23:16:19.18 Sampled 1000.0ml @23:16:19.19 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @23:16:19.20 Intake.select :close @23:16:19.26 CSV.dial :waste @23:16:19.41 SSV.dial CBV,avoiding: :exhaust @23:16:19.45 CS.seek 0ml @23:16:19.47
CBV.dial SSV @23:16:19.57 CSV.dial :air @23:16:20.33 CS.seek 10ml @23:16:21.38
CTV.dial :air @23:16:22.18 delay 15 seconds @23:16:37.20 SSV.dial :spit,avoiding: :exhaust @23:16:38.53 CTV.dial CSV,avoiding: :intake @23:16:38.56
SS.seek 0ml @23:16:38.68 SSV.dial CBV,avoiding: :exhaust @23:16:44.98 SSV.dial between CBV and plug @23:16:45.46 CBV.dial between SSV and waste2 @23:16:46.27 CSV.dial between air and CTV @23:16:46.58 CS.seek 8.082ml @23:16:51.80 CTV.dial CSV,avoiding: :air @23:16:51.82
CSV.dial CTV @23:16:52.22 CS.reconfigure :pressurize @23:16:52.27 CS.seek 0ml @23:17:07.64 BubblePoint: Intake @24.2psia, Outlet @1.4psia @23:17:07.65 CBV.dial SSV @23:17:08.00 SS.reconfigure :default @23:17:08.04 SS.seek 10ml @23:17:13.65 delay 15 seconds @23:17:28.78 Evacuated: Intake @23.7psia, Outlet @23.4psia @23:17:28.79 SSV.dial :spit,avoiding: :exhaust @23:17:29.82 SS.reconfigure :exhale @23:17:29.87 SS.seek 0ml @23:17:34.86 SS.reconfigure :fast @23:17:34.91 SS.seek 5ml @23:17:37.73 SSV.dial CBV,avoiding: :exhaust @23:17:37.75
CBV.dial SSV @23:17:39.10 CTV.dial :air,avoiding: :intake @23:17:40.42 CSV.dial CTV @23:17:40.47
SS.seek 10ml @23:17:41.62 CSV.dial :air @23:17:42.08 CS.reconfigure :fast @23:17:42.12 CS.seek 0ml @23:17:42.96
SSV.dial :spit,avoiding: :exhaust @23:17:44.29 SS.reconfigure :exhale @23:17:44.34 SS.seek 0ml @23:17:48.13 CS.stop @23:17:49.37
SS.reconfigure :fast @23:17:49.42 SS.seek 5ml @23:17:52.23 SSV.dial CBV,avoiding: :exhaust @23:17:52.25
CBV.dial SSV @23:17:54.60 SSV.dial :spit,avoiding: :exhaust @23:17:55.92 SS.reconfigure :default @23:17:55.96 SS.seek 0ml @23:17:59.22 SS.stop @23:17:59.26 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @23:17:59.27 Intake.select :close @23:17:59.34 CSV.dial :waste @23:17:59.46 SSV.dial CBV,avoiding: :exhaust @23:17:59.48
CBV.dial SSV @23:18:00.12 CS.seek 0ml @23:18:00.19 CSV.dial :air @23:18:00.84
CTV.dial :air @23:18:00.92 delay 15 seconds @23:18:01.38 CS.seek 10ml @23:18:15.94
SSV.dial :spit,avoiding: :exhaust @23:18:17.26 CTV.dial CSV,avoiding: :intake @23:18:17.29
SS.reconfigure :exhale @23:18:17.39 SS.seek 0ml @23:18:17.80 SSV.dial CBV,avoiding: :exhaust @23:18:26.04 SSV.dial between CBV and plug @23:18:26.51 CBV.dial between SSV and waste2 @23:18:26.91 CSV.dial between air and CTV @23:18:27.23 CS.seek 7.544ml @23:18:33.70 CTV.dial CSV,avoiding: :air @23:18:33.72
CSV.dial CTV @23:18:34.06 CS.reconfigure :pressurize @23:18:34.11 CS.seek 0ml @23:18:47.83 BubblePoint: Intake @24.2psia, Outlet @3.3psia CBV.dial SSV @23:18:48.17 SS.reconfigure :default @23:18:48.21 SS.seek 10ml @23:18:53.82 delay 15 seconds @23:19:08.95 Evacuated: Intake @24.0psia, Outlet @23.7psia @23:19:08.96 SSV.dial :spit,avoiding: :exhaust @23:19:10.00 SS.reconfigure :exhale @23:19:10.04 SS.seek 0ml @23:19:15.30 SS.reconfigure :fast @23:19:15.35 SS.seek 5ml @23:19:17.93 SSV.dial CBV,avoiding: :exhaust @23:19:17.95
CBV.dial SSV @23:19:19.76 CTV.dial :air,avoiding: :intake @23:19:21.12 CSV.dial CTV @23:19:21.15
SS.seek 10ml @23:19:22.28 CSV.dial :air @23:19:22.73 CS.reconfigure :fast @23:19:22.78 CS.seek 0ml @23:19:23.68
SSV.dial :spit,avoiding: :exhaust @23:19:25.01 SS.reconfigure :exhale @23:19:25.06 SS.seek 0ml @23:19:29.03 CS.stop @23:19:30.20
SS.reconfigure :fast @23:19:30.25 SS.seek 5ml @23:19:33.07 SSV.dial CBV,avoiding: :exhaust @23:19:33.09
CBV.dial SSV @23:19:35.42 SSV.dial :spit,avoiding: :exhaust @23:19:36.74 SS.reconfigure :default @23:19:36.79 SS.seek 0ml @23:19:40.13 SS.stop @23:19:40.17 Lysing: Adding 2.0ml AqMeOH @23:19:40.18 CBV.dial between CSV and waste1,avoiding: :DBV @23:19:41.68 CTV.dial CSV @23:19:43.01 CSV.dial CSR @23:19:43.80 Pulling 1ml of CSR.AqMeOH CSR.select :AqMeOH @23:19:43.91 CS.reconfigure :default @23:19:43.96 CS.seek 1ml @23:19:47.60 delay 5 seconds @23:19:52.62 CSR.select :air @23:19:52.78 CS.seek 6ml @23:20:06.38 delay 2 seconds @23:20:08.40 CSV.dial :waste2 @23:20:08.96 CS.reconfigure :fast @23:20:09.01 CS.seek 0ml @23:20:23.47 CSV.dial CSR @23:20:23.93 Pulling 2ml of CSR.AqMeOH CSR.select :AqMeOH @23:20:24.04 CS.reconfigure :default @23:20:24.09 CS.seek 2ml @23:20:30.00 delay 5 seconds @23:20:35.02 CSR.select :air @23:20:35.19 CS.seek 4.999ml @23:20:43.69 delay 2 seconds @23:20:45.71 CSV.dial CTV @23:20:46.15 CS.seek 1ml @23:20:57.49 Before lysing Collection Puck Heater at 13.7C/26.7psia @23:20:57.51 Heating Puck.from 6, :sh1 in Collection Clamp to 70C for 10:00 @23:20:57.52 CH.seek 70C,8:00 @23:23:06.40 CH.hold 10:00,70C @23:23:06.60 Begun lysing Collection Puck Heater at 68.1C/32.9psia @23:33:06.62 Ended lysing Collection Puck Heater at 70C/32.3psia @23:33:06.63 CS.seek 0ml @23:33:10.09 CTV.dial between spare and air,avoiding: :intake @23:33:11.13 CSV.dial :air @23:33:11.60 CS.seek 5ml @23:33:25.21 CSV.dial CTV @23:33:25.66 delay 2 seconds @23:33:27.68 CSV.dial :air @23:33:28.13 delay 2 seconds @23:33:30.15 CSV.dial CTV @23:33:30.61 delay 2 seconds @23:33:32.63 CSV.dial :air @23:33:33.07 delay 2 seconds @23:33:35.09 CS.seek 0ml @23:33:49.17 Puck @61.9psia after final lysis pressurization @23:33:49.18 Recovering Lysate into Collection syringe @23:33:49.19 CSV.dial CBV @23:33:50.23 CBV.dial CSV @23:33:50.62 CS.seek 5ml @23:34:04.21 delay 5 seconds @23:34:09.23 CTV.dial :air @23:34:09.75 CS.seek 7.001ml @23:34:15.65 delay 5 seconds @23:34:20.67 CSV.dial :air2 @23:34:21.11 CS.seek 10ml @23:34:29.56 CSV.dial between air and CTV,avoiding: CBV @23:34:30.71 Lysing Complete @23:34:30.78 Storing sh1 puck in Collection Clamp to tube 5 @23:34:30.95 SC.reconfigure :tight @23:34:31.00 SC.seek 5 @23:34:34.55 SC.configure :limp @23:34:34.77 Elbow.reconfigure :tight @23:34:34.82 Elbow.seek 5 @23:34:36.59 Forearm.reconfigure :tight @23:34:36.64 Forearm.seek 5 + 450 counts @23:34:37.67 SE.reconfigure :pick @23:34:37.73 SE.seek :beamBreak @23:34:40.40 SE.configure :default @23:34:40.55 Elbow.reconfigure :limp @23:34:40.60 Forearm.reconfigure :loose @23:34:40.64 Forearm.seek 5 @23:34:40.90 Elbow.hold @23:34:40.96 Elbow.reconfigure :tight @23:34:41.06 SE.seek 7.15 Pucks below Top Plate @23:34:41.60 Forearm.force -20 @23:34:41.64 Forearm.seek 5 + 278 counts @23:34:41.92 Forearm.reconfigure :tight @23:34:41.97 Forearm.seek 5 + 450 counts @23:34:42.20 SE.seek 8.15 Pucks below Top Plate @23:34:42.86 Forearm.reconfigure :loose @23:34:42.91 Forearm.seek :retract @23:34:43.90 Elbow.seek CC @23:34:45.60 Elbow.reconfigure :loose @23:34:45.70 Forearm.seek CC @23:34:46.65 Hand.close @23:34:47.21 CC.seek :open @23:34:51.94 Forearm.seek :retract @23:34:52.92 Elbow.reconfigure :tight @23:34:52.96 Elbow.seek 5 @23:34:54.71 Elbow.reconfigure :limp @23:34:54.76 Forearm.seek 5 @23:34:55.80 Elbow.hold @23:34:55.87 Elbow.reconfigure :tight @23:34:55.92 Hand.open @23:34:56.71 Forearm.force -20 @23:34:56.75 Forearm.seek 5 + 312 counts @23:34:57.05 Forearm.reconfigure :tight @23:34:57.10 Forearm.seek 5 + 450 counts @23:34:57.37 SE.seek :down @23:34:58.50
Forearm.reconfigure :loose @23:34:58.55 Forearm.seek :retract @23:34:59.53 SE.stop @23:34:59.57
Tranferring DA @23:34:59.58 Beginning samplePassC @23:34:59.60 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @23:34:59.78 Elbow.seek FlushPuck::Garage @23:35:01.59 Elbow.reconfigure :loose @23:35:02.11 Forearm.seek :garage @23:35:03.15 Hand.close @23:35:03.83 Forearm.seek :retract @23:35:04.84 Elbow.reconfigure :tight @23:35:04.88 Elbow.seek CC @23:35:07.87 Elbow.reconfigure :loose @23:35:07.97 Forearm.reconfigure :tight @23:35:08.02 Forearm.seek CC @23:35:09.22 CC.seek :closed @23:35:13.74 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.37 @23:35:13.81 Hand.open @23:35:14.52 Forearm.reconfigure :loose @23:35:14.57 Forearm.seek :retract @23:35:15.57 PSV.dial PSR @23:35:16.96 Pulling 1.5ml of PSR.flush PSR.select :flush @23:35:17.08 PS.seek 1.5ml @23:35:33.31 delay 5 seconds @23:35:38.32 PSV.dial :waste1 @23:35:38.81 PS.seek 0ml @23:35:54.93 PSV.dial PSR @23:35:55.40 PSR.select :air @23:35:55.52 PS.seek 2.5ml @23:36:21.94 PRV.dial CBV,avoiding: :ab2 @23:36:21.96
CBV.dial PRV @23:36:23.69 PSV.dial :mixloop @23:36:24.74 CTV.dial :air,avoiding: :intake @23:36:25.35 PTV.dial :PRVmixing @23:36:26.75 PS.seek 0ml @23:36:53.27 PSV.dial :air @23:36:53.86 PS.seek 2.5ml @23:37:20.15 PSV.dial :mixloop @23:37:20.61 PS.seek 0ml @23:37:46.99 PSV.dial :air @23:37:47.47 PS.seek 2.5ml @23:38:13.96 PSV.dial :mixloop @23:38:14.43 PS.seek 0ml @23:38:40.77 Garaging FlushPuck in Collection Clamp @23:38:40.84 Elbow.reconfigure :tight @23:38:40.89 Elbow.seek CC @23:38:41.19 Elbow.reconfigure :loose @23:38:41.29 Forearm.seek CC @23:38:42.36 Hand.close @23:38:42.96 CC.seek :open @23:38:47.60 Forearm.seek :retract @23:38:48.62 Elbow.reconfigure :tight @23:38:48.66 Elbow.seek :garage @23:38:51.58 Elbow.reconfigure :loose @23:38:51.67 Forearm.reconfigure :tight @23:38:51.72 Forearm.seek :garage @23:38:52.79 Hand.open @23:38:53.52 Forearm.reconfigure :loose @23:38:53.56 Forearm.seek :retract @23:38:54.55 PSV.dial :mixloop @23:38:54.63 PTV.dial :PRVmixing @23:38:54.64 PRV.dial CBV @23:38:54.68
CBV.dial PRV @23:38:54.81 CTV.dial :air,avoiding: :intake @23:38:54.83 Loading lyfil puck from tube 6 into Collection Clamp @23:38:55.06 SC.reconfigure :tight @23:38:55.11 SC.seek 6 @23:38:58.73 SC.configure :limp @23:38:59.38 Elbow.reconfigure :tight @23:38:59.43 Elbow.seek 6 @23:39:01.36 Forearm.reconfigure :tight @23:39:01.41 Forearm.seek 6 + 450 counts @23:39:02.50 SE.reconfigure :pick @23:39:02.56 SE.seek :beamBreak @23:39:04.09 SE.configure :default @23:39:04.25 Elbow.seek 6 @23:39:04.44 Forearm.seek 6 + 300 counts @23:39:04.60 Forearm.reconfigure :loose @23:39:04.64 Hand.close @23:39:05.22 SE.seek :down @23:39:05.41
Forearm.seek :retract @23:39:06.40 Elbow.seek CC @23:39:06.46 SE.stop @23:39:08.04
Elbow.reconfigure :loose @23:39:08.14 Forearm.reconfigure :tight @23:39:08.19 Forearm.seek CC @23:39:09.44 CC.seek :closed @23:39:13.96 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.19 @23:39:13.97 Hand.open @23:39:15.10 Forearm.reconfigure :loose @23:39:15.15 Forearm.seek :retract @23:39:16.16 CTV.dial CSV,avoiding: :intake @23:39:17.49 CSV.dial CTV @23:39:17.81 PS.seek 2.5ml @23:39:44.17 CS.seek 4.5ml @23:39:59.02 delay 8 seconds @23:40:07.04 PSV.dial :air @23:40:07.50 delay 2 seconds @23:40:09.52 PSV.dial :waste2 @23:40:10.26 PS.seek 0ml @23:40:36.58 CBV.dial between PRV and waste1 @23:40:36.91 CSV.dial :air @23:40:37.37 CS.seek 10ml @23:40:52.20 CSV.dial CTV @23:40:52.66 CS.seek 0ml @23:41:19.23 CBV.dial :waste1 @23:41:19.55 delay 5 seconds @23:41:24.57 CTV.dial :air,avoiding: :intake @23:41:26.00 Storing lyfil puck in Collection Clamp to tube 5 @23:41:26.17 SC.reconfigure :tight @23:41:26.22 SC.seek 5 @23:41:29.77 SC.configure :limp @23:41:29.98 Elbow.reconfigure :tight @23:41:30.03 Elbow.seek 5 @23:41:31.78 Forearm.reconfigure :tight @23:41:31.83 Forearm.seek 5 + 450 counts @23:41:32.86 SE.reconfigure :pick @23:41:32.92 SE.seek :beamBreak @23:41:35.45 SE.configure :default @23:41:35.61 Elbow.reconfigure :limp @23:41:35.66 Forearm.reconfigure :loose @23:41:35.70 Forearm.seek 5 @23:41:35.94 Elbow.hold @23:41:36.44 Elbow.reconfigure :tight @23:41:36.54 SE.seek 8.17 Pucks below Top Plate @23:41:37.03 Forearm.force -20 @23:41:37.08 Forearm.seek 5 + 302 counts @23:41:37.37 Forearm.reconfigure :tight @23:41:37.42 Forearm.seek 5 + 450 counts @23:41:37.62 SE.seek 9.17 Pucks below Top Plate @23:41:38.24 Forearm.reconfigure :loose @23:41:38.29 Forearm.seek :retract @23:41:39.22 Elbow.seek CC @23:41:40.93 Elbow.reconfigure :loose @23:41:41.02 Forearm.seek CC @23:41:41.98 Hand.close @23:41:42.58 CC.seek :open @23:41:47.31 Forearm.seek :retract @23:41:48.30 Elbow.reconfigure :tight @23:41:48.35 Elbow.seek 5 @23:41:50.10 Elbow.reconfigure :limp @23:41:50.15 Forearm.seek 5 @23:41:51.16 Elbow.hold @23:41:51.22 Elbow.reconfigure :tight @23:41:51.27 Hand.open @23:41:51.95 Forearm.force -20 @23:41:51.99 Forearm.seek 5 + 319 counts @23:41:52.29 Forearm.reconfigure :tight @23:41:52.71 Forearm.seek 5 + 450 counts @23:41:53.04 SE.seek :down @23:41:54.17
Forearm.reconfigure :loose @23:41:54.22 Forearm.seek :retract @23:41:55.18 SE.stop @23:41:55.22
PSV.dial :air @23:41:55.98 PS.seek 2.5ml @23:42:22.36 delay 1 second @23:42:23.37 PSV.dial :mixloop @23:42:23.83 delay 1 second @23:42:24.85 PSV.dial :air @23:42:25.44 delay 1 second @23:42:26.45 PSV.dial :mixloop @23:42:26.91 delay 1 second @23:42:27.92 PSV.dial :air @23:42:28.39 delay 1 second @23:42:29.41 PS.seek 0ml @23:42:55.80 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @23:42:55.98 Elbow.seek FlushPuck::Garage @23:42:57.85 Elbow.reconfigure :loose @23:42:57.95 Forearm.seek :garage @23:42:58.94 Hand.close @23:42:59.70 Forearm.seek :retract @23:43:00.68 Elbow.reconfigure :tight @23:43:00.72 Elbow.seek PC @23:43:02.00 Elbow.reconfigure :loose @23:43:02.10 Forearm.reconfigure :tight @23:43:02.14 Forearm.seek PC @23:43:03.34 PC.seek :closed @23:43:07.96 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.63 @23:43:08.03 Hand.open @23:43:08.64 Forearm.reconfigure :loose @23:43:08.69 Forearm.seek :retract @23:43:09.66 PRV.dial :air @23:43:10.11 PSV.dial :mixloop @23:43:10.64 PS.seek 0.3ml @23:43:14.55 PSV.dial PSR @23:43:15.59 Pulling 0.4ml of PSR.air @23:43:15.60 PSR.select :air @23:43:15.63 PS.seek 0.7ml @23:43:20.52 delay 3 seconds @23:43:23.53 PSR.select :air @23:43:23.56 PS.seek 2.5ml @23:43:42.73 delay 3 seconds @23:43:45.75 PSV.dial :mixloop @23:43:46.81 PTV.dial :mixing @23:43:47.56 PBV.dial :waste2 @23:43:48.54 PS.seek 0ml @23:44:14.89 Garaging FlushPuck in Processing Clamp @23:44:14.96 Elbow.reconfigure :tight @23:44:15.01 Elbow.seek PC @23:44:15.13 Elbow.reconfigure :loose @23:44:15.23 Forearm.seek PC @23:44:16.17 Hand.close @23:44:16.83 PC.seek :open @23:44:21.54 Forearm.seek :retract @23:44:22.53 Elbow.reconfigure :tight @23:44:22.58 Elbow.seek :garage @23:44:23.96 Elbow.reconfigure :loose @23:44:24.06 Forearm.reconfigure :tight @23:44:24.11 Forearm.seek :garage @23:44:25.18 Hand.open @23:44:25.89 Forearm.reconfigure :loose @23:44:25.93 Forearm.seek :retract @23:44:26.94 Loading da puck from tube 6 into Processing Clamp @23:44:27.61 SC.reconfigure :tight @23:44:27.66 SC.seek 6 @23:44:31.23 SC.configure :limp @23:44:31.44 Elbow.reconfigure :tight @23:44:31.49 Elbow.seek 6 @23:44:33.35 Forearm.reconfigure :tight @23:44:33.40 Forearm.seek 6 + 450 counts @23:44:34.45 SE.reconfigure :pick @23:44:34.51 SE.seek :beamBreak @23:44:36.23 SE.configure :default @23:44:36.39 Elbow.seek 6 @23:44:36.57 Forearm.seek 6 + 300 counts @23:44:36.78 Forearm.reconfigure :loose @23:44:36.83 Hand.close @23:44:37.47 SE.seek :down @23:44:37.67
Forearm.seek :retract @23:44:38.62 Elbow.seek PC @23:44:38.77 SE.stop @23:44:41.18
Elbow.reconfigure :loose @23:44:41.28 Forearm.reconfigure :tight @23:44:41.32 Forearm.seek PC @23:44:42.61 PC.seek :closed @23:44:47.32 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.64 @23:44:47.33 Hand.open @23:44:48.46 Forearm.reconfigure :loose @23:44:48.50 Forearm.seek :retract @23:44:49.52 Heating Puck.from 6, :da in Processing Clamp to 28C for 2:00:00 @23:44:49.54 PH.seek 28C,5:00 @23:45:30.73 PH.hold 2:00:00,28C @23:45:30.78 delay 1:30 @23:47:00.82 PSR.select :air @23:47:00.84 PSV.dial PSR @23:47:01.90 Pulling 0.5ml of PSR.wash @23:47:01.91 PSR.select :wash @23:47:02.03 PS.seek 0.5ml @23:47:07.98 delay 5 seconds @23:47:13.00 PSR.select :air @23:47:13.12 PS.seek 2ml @23:47:29.26 PTV.dial :puck @23:47:30.01 PSV.dial PTV @23:47:31.21 PBV.dial :waste1 @23:47:32.61 PS.seek 1.5ml @23:47:38.57 delay 3 seconds @23:47:41.59 PBV.dial between PSV and waste1 @23:47:41.94 PS.seek 0ml @23:47:58.07 delay 5:00 @23:52:58.10 recoverPSRreagent08 @23:52:58.11 PTV.dial :puck @23:52:58.18 PBV.dial :waste1 @23:52:58.54 delay 30 seconds @23:53:28.56 PBV.dial between PSV and waste1 @23:53:28.93 PSR.select :air @23:53:28.94 PSV.dial PSR @23:53:29.71 PS.reconfigure :fast @23:53:29.76 PS.seek 2.5ml @23:53:53.41 PSV.dial PTV @23:53:54.18 delay 5 seconds @23:53:59.20 PSV.dial PSR @23:53:59.96 delay 5 seconds @23:54:04.98 PSV.dial PTV @23:54:05.74 delay 5 seconds @23:54:10.76 PSV.dial :air @23:54:11.53 PS.seek 0ml @23:54:34.75 PTV.dial :PRVmixing,via: PRV @23:54:36.08 PSV.dial :mixloop @23:54:36.55 PRV.dial :ab1 @23:54:37.29 PS.reconfigure :slow @23:54:37.34 PS.seek 0.15ml @23:54:40.88 delay 2 seconds @23:54:42.90 CBV.dial PRV @23:54:42.92
PRV.dial CBV @23:54:43.50 PS.seek 0.35ml @23:54:48.13 delay 2 seconds @23:54:50.14 CBV.dial between PRV and PSV @23:54:50.47 PRV.dial :air @23:54:50.93 PS.seek 0.9ml @23:55:02.72 PRV.dial :waste2 @23:55:03.46 PS.seek 0.5ml @23:55:12.19 addPMLReagent08 @23:55:12.20 PSV.dial :air @23:55:12.66 PS.reconfigure :default @23:55:12.71 PS.seek 2ml @23:55:28.94 PSV.dial :PML @23:55:29.41 PTV.dial :mixing @23:55:30.16 PBV.dial :waste1 @23:55:30.60 PS.seek 1.85ml @23:55:33.00 PBV.dial between PSV and waste1 @23:55:33.38 PS.seek 0ml @23:55:53.56 delay 2 seconds @23:55:55.58 Beginning samplePassC @23:55:55.60 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @23:55:55.78 Elbow.reconfigure :tight @23:55:55.83 Elbow.seek FlushPuck::Garage @23:55:57.17 Elbow.reconfigure :loose @23:55:57.27 Forearm.seek :garage @23:55:58.27 Hand.close @23:55:59.06 Forearm.seek :retract @23:56:00.06 Elbow.reconfigure :tight @23:56:00.10 Elbow.seek CC @23:56:03.02 Elbow.reconfigure :loose @23:56:03.12 Forearm.reconfigure :tight @23:56:03.16 Forearm.seek CC @23:56:04.38 CC.seek :closed @23:56:08.91 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.51 @23:56:08.97 Hand.open @23:56:09.63 Forearm.reconfigure :loose @23:56:09.68 Forearm.seek :retract @23:56:10.61 PSV.dial PSR @23:56:11.67 Pulling 1.5ml of PSR.flush @23:56:11.68 PSR.select :flush @23:56:11.80 PS.seek 1.5ml @23:56:27.94 delay 5 seconds @23:56:32.96 PSV.dial :waste1 @23:56:33.41 PS.seek 0ml @23:56:49.59 PSV.dial PSR @23:56:50.10 PSR.select :air @23:56:50.22 PS.seek 2.5ml @23:57:16.54 PRV.dial CBV,avoiding: :ab2 @23:57:16.56
CBV.dial PRV @23:57:18.19 PSV.dial :mixloop @23:57:19.24 CTV.dial :air,avoiding: :intake @23:57:19.41 PTV.dial :PRVmixing @23:57:20.18 PS.seek 0ml @23:57:46.53 PSV.dial :air @23:57:47.01 PS.seek 2.5ml @23:58:13.31 PSV.dial :mixloop @23:58:13.80 PS.seek 0ml @23:58:40.15 PSV.dial :air @23:58:40.63 PS.seek 2.5ml @23:59:07.03 PSV.dial :mixloop @23:59:07.50 PS.seek 0ml @23:59:33.94 Garaging FlushPuck in Collection Clamp @23:59:34.01 Elbow.reconfigure :tight @23:59:34.05 Elbow.seek CC @23:59:34.33 Elbow.reconfigure :loose @23:59:34.42 Forearm.seek CC @23:59:35.45 Hand.close @23:59:36.07 CC.seek :open @23:59:40.71 Forearm.seek :retract @23:59:41.69 Elbow.reconfigure :tight @23:59:41.74 Elbow.seek :garage @23:59:44.69 Elbow.reconfigure :loose @23:59:44.78 Forearm.reconfigure :tight @23:59:44.83 Forearm.seek :garage @23:59:45.87 Hand.open @23:59:46.50 Forearm.reconfigure :loose @23:59:46.55 Forearm.seek :retract @23:59:47.51 delayUntil "00:03:55.57UTC09-Dec-23" @23:59:47.53 Whole Cell Archive sampling 1000ml @23:59:47.55 Loading wcr puck from tube 6 into Collection Clamp @23:59:48.47 Elbow.reconfigure :tight @23:59:48.52 Elbow.seek 6 @23:59:50.53 Forearm.reconfigure :tight @23:59:50.59 Forearm.seek 6 + 450 counts @23:59:51.62 SE.reconfigure :pick @23:59:51.68 SE.seek :beamBreak @23:59:53.39 SE.configure :default @23:59:53.56 Elbow.seek 6 @23:59:53.75 Forearm.seek 6 + 300 counts @23:59:53.99 Forearm.reconfigure :loose @23:59:54.04 Hand.close @23:59:54.71 SE.seek :down @23:59:54.91 Forearm.seek :retract @23:59:55.85 Elbow.seek CC @23:59:56.12 SE.stop @23:59:57.48 Elbow.reconfigure :loose @23:59:57.59 Forearm.reconfigure :tight @23:59:57.64 Forearm.seek CC @23:59:58.93 CC.seek :closed @00:00:03.45UTC09-Dec-23 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.29 @00:00:03.46 Hand.open @00:00:04.13 Forearm.reconfigure :loose @00:00:04.18 Forearm.seek :retract @00:00:05.12 collectSample 1000ml w/bubble point of 35psi @00:00:05.13 Priming 7ml @00:00:05.14 Intake.select :close @00:00:05.15 Exhaust.select :close @00:00:05.16 SS.hold @00:00:05.38 Calibrating pressure sensors @00:00:05.40 CTV.dial :air @00:00:05.42 SSV.dial :spit,avoiding: :exhaust @00:00:05.51 SS.reconfigure :fast @00:00:05.57 SS.seek 5ml @00:00:08.24 SSV.dial CBV @00:00:08.26 CBV.dial SSV @00:00:09.63 delay 3.8 seconds @00:00:13.57 Sensor limits: Intake max 101.1psia; Outlet max 93.1psia @00:00:13.58 SSV.dial :spit @00:00:14.92 SS.seek 0ml @00:00:17.78 SSV.dial CBV @00:00:19.11 CTV.dial :intake @00:00:19.89 SSV.dial CBV @00:00:19.92 CBV.dial SSV @00:00:20.05 Allowing 40s for 7.0ml of remaining 7.0ml @00:00:20.07 Intake.select :open @00:00:22.23 Intake @14.7psia, vacuum from 9.2 to 12.2psi @00:00:22.25 SS.reconfigure :inhaling 2.20ml/s @00:00:22.30 SS.seek 7ml @00:00:28.98 Stroke completed -- waiting for pressure to equalize ... @00:00:29.00 SS.reconfigure :exhale @00:00:29.04 SS.hold @00:00:47.41 Intake.select :close @00:00:47.57 SSV.dial :exhaust @00:00:48.35 Exhaust.select :open @00:00:48.46 SS.seek 0ml @00:00:52.56 Exhaust.select :close @00:00:52.67 Sampling 1000.0ml @00:00:52.69 Intake.select :close @00:00:52.70 Exhaust.select :close @00:00:52.71 SS.reconfigure :default @00:00:52.75 SS.hold @00:00:52.96 CTV.dial :intake @00:00:53.13 SSV.dial CBV @00:00:53.15 CBV.dial SSV @00:00:53.94 Allowing 130s for 25.0ml of remaining 1000.0ml @00:00:53.95 Intake.select :open @00:00:56.12 Intake @15.7psia, vacuum from 10.0 to 13.0psi @00:00:56.14 SS.reconfigure :inhaling 2.20ml/s @00:00:56.19 SS.seek 25ml @00:01:03.07 Vacuum too high -- waiting up to 40 seconds @00:01:03.09 SS.reconfigure :exhale @00:01:03.14 SS.hold @00:01:10.39 Intake @19.1psia, vacuum from 10.0 to 13.0psi @00:01:10.41 SS.reconfigure :inhaling 1.76ml/s @00:01:10.46 SS.seek 25ml @00:01:12.60 Vacuum too high -- waiting up to 40 seconds @00:01:12.62 SS.reconfigure :exhale @00:01:12.68 SS.hold @00:01:14.95 Intake @19.3psia, vacuum from 10.0 to 13.0psi @00:01:14.97 SS.reconfigure :inhaling 1.41ml/s @00:01:15.02 SS.seek 25ml @00:01:17.10 Vacuum too high -- waiting up to 40 seconds @00:01:17.12 SS.reconfigure :exhale @00:01:17.17 SS.hold @00:01:19.03 Intake @19.4psia, vacuum from 10.0 to 13.0psi @00:01:19.05 SS.reconfigure :inhaling 1.13ml/s @00:01:19.09 SS.seek 25ml @00:01:21.38 Vacuum too high -- waiting up to 40 seconds @00:01:21.40 SS.reconfigure :exhale @00:01:21.45 SS.hold @00:01:23.18 SS.reconfigure :inhaling 0.91ml/s @00:01:23.24 SS.seek 25ml @00:01:26.07 Vacuum too high -- waiting up to 40 seconds @00:01:26.09 SS.reconfigure :exhale @00:01:26.14 SS.hold @00:01:27.91 Intake @19.5psia, vacuum from 10.0 to 13.0psi @00:01:27.93 SS.reconfigure :inhaling 0.73ml/s @00:01:27.98 SS.seek 25ml @00:01:32.45 Vacuum too high -- waiting up to 40 seconds @00:01:32.46 SS.reconfigure :exhale @00:01:32.52 SS.hold @00:01:34.23 Intake @19.4psia, vacuum from 10.0 to 13.0psi @00:01:34.25 SS.reconfigure :inhaling 0.59ml/s @00:01:34.30 SS.seek 25ml @00:01:42.25 Stroke completed -- waiting for pressure to equalize ... @00:01:42.26 SS.reconfigure :exhale @00:01:42.75 SS.hold @00:02:17.51 Intake.select :close @00:02:17.67 SSV.dial :exhaust @00:02:18.45 Exhaust.select :open @00:02:18.57 SS.seek 0ml @00:02:27.37 Exhaust.select :close @00:02:27.49 SSV.dial CBV @00:02:27.51 CBV.dial SSV @00:02:28.30 Allowing 130s for 25.0ml of remaining 975.0ml @00:02:28.31 Intake.select :open @00:02:30.48 Intake @16.1psia, vacuum from 10.0 to 13.0psi @00:02:30.50 SS.reconfigure :inhaling 0.59ml/s @00:02:30.55 SS.seek 25ml @00:03:15.54 Stroke completed -- waiting for pressure to equalize ... @00:03:15.56 SS.reconfigure :exhale @00:03:15.60 SS.hold @00:03:50.95 Intake.select :close @00:03:51.11 SSV.dial :exhaust @00:03:51.87 Exhaust.select :open @00:03:51.99 SS.seek 0ml @00:03:55.58 recoverPRVreagent08 @00:03:55.59 PTV.dial :mixing @00:03:56.36 PBV.dial :waste1 @00:03:56.73 delay 30 seconds @00:04:00.84 Exhaust.select :close @00:04:00.96 SSV.dial CBV @00:04:00.98 CBV.dial SSV @00:04:01.77 Allowing 130s for 25.0ml of remaining 950.0ml @00:04:01.79 Intake.select :open @00:04:03.95 Intake @16.2psia, vacuum from 10.0 to 13.0psi @00:04:03.97 SS.reconfigure :inhaling 0.59ml/s @00:04:04.02 SS.seek 25ml @00:04:26.75 PBV.dial between PSV and waste1 @00:04:27.12 PSV.dial :air @00:04:27.59 PS.reconfigure :fast @00:04:27.64 PS.seek 2.5ml @00:04:48.84 Stroke completed -- waiting for pressure to equalize ... @00:04:48.85 SS.reconfigure :exhale @00:04:48.90 SS.hold @00:04:51.63 PSV.dial :mixloop @00:04:52.14 delay 2 seconds @00:04:54.16 PSV.dial :air @00:04:54.77 delay 2 seconds @00:04:56.79 PSV.dial :mixloop @00:04:57.24 delay 2 seconds @00:04:59.26 PSV.dial :air @00:04:59.73 PS.seek 0ml @00:05:21.95 Intake.select :close @00:05:22.11 SSV.dial :exhaust @00:05:22.88 Exhaust.select :open @00:05:23.00 SS.seek 0ml @00:05:23.12 washCycleDA #1 of 2 @00:05:23.13 PSR.select :air @00:05:23.14 PSV.dial PSR @00:05:24.49 Pulling 0.5ml of PSR.wash @00:05:24.50 PSR.select :wash @00:05:24.62 PS.reconfigure :default @00:05:24.67 PS.seek 0.5ml @00:05:30.68 delay 5 seconds @00:05:31.76 Exhaust.select :close @00:05:31.88 SSV.dial CBV @00:05:31.90 CBV.dial SSV @00:05:32.67 Allowing 130s for 25.0ml of remaining 925.0ml @00:05:32.68 Intake.select :open @00:05:34.85 Intake @16.3psia, vacuum from 10.0 to 13.0psi @00:05:34.87 SS.reconfigure :inhaling 0.59ml/s @00:05:34.92 SS.seek 25ml @00:05:35.69 PSR.select :air @00:05:35.82 PS.seek 2ml @00:05:52.02 PTV.dial :puck @00:05:52.77 PSV.dial PTV @00:05:53.52 PBV.dial :waste1 @00:05:53.96 PS.seek 1.5ml @00:05:59.86 delay 3 seconds @00:06:02.88 PBV.dial between PSV and waste1 @00:06:03.26 PS.seek 0ml @00:06:19.37 delay 2:00 @00:06:19.92 Stroke completed -- waiting for pressure to equalize ... @00:06:19.93 SS.reconfigure :exhale @00:06:19.98 SS.hold @00:06:49.06 Intake.select :close @00:06:49.22 SSV.dial :exhaust @00:06:50.00 Exhaust.select :open @00:06:50.11 SS.seek 0ml @00:06:59.24 Exhaust.select :close @00:06:59.36 SSV.dial CBV @00:06:59.38 CBV.dial SSV @00:07:00.17 Allowing 130s for 25.0ml of remaining 900.0ml @00:07:00.18 Intake.select :open @00:07:02.36 Intake @16.2psia, vacuum from 10.0 to 13.0psi @00:07:02.37 SS.reconfigure :inhaling 0.59ml/s @00:07:02.42 SS.seek 25ml @00:07:47.43 Stroke completed -- waiting for pressure to equalize ... @00:07:47.44 SS.reconfigure :exhale @00:07:47.49 SS.hold @00:08:16.37 Intake.select :close @00:08:16.53 SSV.dial :exhaust @00:08:17.31 Exhaust.select :open @00:08:17.42 SS.seek 0ml @00:08:19.38 recoverPSRreagent08 @00:08:19.40 PTV.dial :puck @00:08:19.93 PBV.dial :waste1 @00:08:20.30 delay 30 seconds @00:08:26.21 Exhaust.select :close @00:08:26.32 SSV.dial CBV @00:08:26.35 CBV.dial SSV @00:08:27.12 Allowing 130s for 25.0ml of remaining 875.0ml @00:08:27.14 Intake.select :open @00:08:29.30 Intake @16.3psia, vacuum from 10.0 to 13.0psi @00:08:29.32 SS.reconfigure :inhaling 0.59ml/s @00:08:29.37 SS.seek 25ml @00:08:50.33 PBV.dial between PSV and waste1 @00:08:50.71 PSR.select :air @00:08:50.72 PSV.dial PSR @00:08:51.49 PS.reconfigure :fast @00:08:51.54 PS.seek 2.5ml @00:09:14.24 Stroke completed -- waiting for pressure to equalize ... @00:09:14.25 SS.reconfigure :exhale @00:09:14.30 SS.hold @00:09:15.45 PSV.dial PTV @00:09:16.22 delay 5 seconds @00:09:21.24 PSV.dial PSR @00:09:22.00 delay 5 seconds @00:09:27.07 PSV.dial PTV @00:09:27.95 delay 5 seconds @00:09:32.97 PSV.dial :air @00:09:33.87 PS.seek 0ml @00:09:38.92 Intake.select :close @00:09:39.07 SSV.dial :exhaust @00:09:39.84 Exhaust.select :open @00:09:39.95 SS.seek 0ml @00:09:48.74 Exhaust.select :close @00:09:48.85 SSV.dial CBV @00:09:48.89 CBV.dial SSV @00:09:49.67 Allowing 130s for 25.0ml of remaining 850.0ml @00:09:49.68 Intake.select :open @00:09:51.85 Intake @16.2psia, vacuum from 10.0 to 13.0psi @00:09:51.87 SS.reconfigure :inhaling 0.59ml/s @00:09:51.92 SS.seek 25ml @00:09:57.61 washCycleDA #2 of 2 @00:09:57.62 PSR.select :air @00:09:57.64 PSV.dial PSR @00:09:58.99 Pulling 0.5ml of PSR.wash @00:09:59.00 PSR.select :wash @00:09:59.11 PS.reconfigure :default @00:09:59.16 PS.seek 0.5ml @00:10:05.05 delay 5 seconds @00:10:10.07 PSR.select :air @00:10:10.19 PS.seek 2ml @00:10:26.42 PTV.dial :puck @00:10:26.50 PSV.dial PTV @00:10:27.27 PBV.dial :waste1 @00:10:27.70 PS.seek 1.5ml @00:10:33.67 delay 3 seconds @00:10:36.69 PBV.dial between PSV and waste1 @00:10:36.79 Stroke completed -- waiting for pressure to equalize ... @00:10:36.80 SS.reconfigure :exhale @00:10:36.85 SS.hold @00:10:37.07 PS.seek 0ml @00:10:53.17 delay 2:00 @00:11:01.66 Intake.select :close @00:11:01.82 SSV.dial :exhaust @00:11:02.60 Exhaust.select :open @00:11:02.72 SS.seek 0ml @00:11:11.69 Exhaust.select :close @00:11:11.81 SSV.dial CBV @00:11:11.83 CBV.dial SSV @00:11:12.99 Allowing 130s for 25.0ml of remaining 825.0ml @00:11:13.07 Intake.select :open @00:11:15.24 Intake @16.3psia, vacuum from 10.0 to 13.0psi @00:11:15.26 SS.reconfigure :inhaling 0.59ml/s @00:11:15.31 SS.seek 25ml @00:11:59.61 Stroke completed -- waiting for pressure to equalize ... @00:11:59.62 SS.reconfigure :exhale @00:11:59.68 SS.hold @00:12:22.12 Intake.select :close @00:12:22.73 SSV.dial :exhaust @00:12:23.50 Exhaust.select :open @00:12:23.62 SS.seek 0ml @00:12:32.46 Exhaust.select :close @00:12:32.57 SSV.dial CBV @00:12:32.61 CBV.dial SSV @00:12:33.38 Allowing 130s for 25.0ml of remaining 800.0ml @00:12:33.39 Intake.select :open @00:12:35.56 Intake @16.4psia, vacuum from 10.0 to 13.0psi @00:12:35.58 SS.reconfigure :inhaling 0.59ml/s @00:12:35.63 SS.seek 25ml @00:12:53.20 recoverPSRreagent08 @00:12:53.21 PTV.dial :puck @00:12:53.28 PBV.dial :waste1 @00:12:53.66 delay 30 seconds @00:13:20.41 Stroke completed -- waiting for pressure to equalize ... @00:13:20.42 SS.reconfigure :exhale @00:13:20.47 SS.hold @00:13:23.67 PBV.dial between PSV and waste1 @00:13:24.08 PSR.select :air @00:13:24.10 PSV.dial PSR @00:13:24.86 PS.reconfigure :fast @00:13:24.91 PS.seek 2.5ml @00:13:42.97 Intake.select :close @00:13:43.13 SSV.dial :exhaust @00:13:43.89 Exhaust.select :open @00:13:44.01 SS.seek 0ml @00:13:48.89 PSV.dial PTV @00:13:49.65 delay 5 seconds @00:13:52.78 Exhaust.select :close @00:13:52.89 SSV.dial CBV @00:13:52.91 CBV.dial SSV @00:13:53.71 Allowing 130s for 25.0ml of remaining 775.0ml @00:13:53.72 Intake.select :open @00:13:54.67 PSV.dial PSR @00:13:55.45 delay 5 seconds @00:13:55.89 Intake @16.3psia, vacuum from 10.0 to 13.0psi @00:13:55.91 SS.reconfigure :inhaling 0.59ml/s @00:13:55.96 SS.seek 25ml @00:14:00.47 PSV.dial PTV @00:14:01.25 delay 5 seconds @00:14:06.27 PSV.dial :air @00:14:07.03 PS.seek 0ml @00:14:30.45 getPMLReagent08 @00:14:30.46 PRV.dial :air @00:14:30.92 delay 5 seconds @00:14:35.94 PTV.dial :PRVmixing @00:14:37.26 delay 5 seconds @00:14:40.72 Stroke completed -- waiting for pressure to equalize ... @00:14:40.73 SS.reconfigure :exhale @00:14:40.78 SS.hold @00:14:42.28 PRV.dial :ab2 @00:14:42.74 PTV.dial :PRVmixing @00:14:43.31 PSV.dial :mixloop @00:14:43.84 PS.reconfigure :default @00:14:43.94 PS.seek 0.3ml @00:14:47.88 delay 2 seconds @00:14:49.90 PRV.dial :air @00:14:50.43 PS.seek 0.55ml @00:14:53.79 addPMLReagent08 @00:14:53.80 PSV.dial :air @00:14:54.27 PS.seek 2ml @00:15:03.96 Intake.select :close @00:15:04.12 SSV.dial :exhaust @00:15:04.89 Exhaust.select :open @00:15:05.01 SS.seek 0ml @00:15:09.86 PSV.dial :PML @00:15:10.37 PTV.dial :mixing @00:15:11.14 PBV.dial :waste1 @00:15:12.04 PS.seek 1.85ml @00:15:14.02 Exhaust.select :close @00:15:14.13 SSV.dial CBV @00:15:14.16 CBV.dial SSV @00:15:14.45 PBV.dial between PSV and waste1 @00:15:14.83 PS.seek 0ml @00:15:14.93 Allowing 130s for 25.0ml of remaining 750.0ml @00:15:14.94 Intake.select :open @00:15:17.11 Intake @16.3psia, vacuum from 10.0 to 13.0psi @00:15:17.13 SS.reconfigure :inhaling 0.59ml/s @00:15:17.18 SS.seek 25ml @00:15:34.76 delay 2 seconds @00:15:36.78 delay 8:00 @00:16:00.09 Vacuum too high -- waiting up to 40 seconds @00:16:00.10 SS.reconfigure :exhale @00:16:00.16 SS.hold @00:16:04.41 Intake @16.6psia, vacuum from 10.0 to 13.0psi @00:16:04.43 SS.reconfigure :inhaling 0.47ml/s @00:16:04.48 SS.seek 25ml @00:16:06.64 Stroke completed -- waiting for pressure to equalize ... @00:16:06.65 SS.reconfigure :exhale @00:16:06.70 SS.hold @00:16:23.47 Intake.select :close @00:16:23.63 SSV.dial :exhaust @00:16:24.39 Exhaust.select :open @00:16:24.51 SS.seek 0ml @00:16:33.26 Exhaust.select :close @00:16:33.37 SSV.dial CBV @00:16:33.40 CBV.dial SSV @00:16:34.17 Allowing 130s for 25.0ml of remaining 725.0ml @00:16:34.19 Intake.select :open @00:16:36.35 Intake @16.4psia, vacuum from 10.0 to 13.0psi @00:16:36.37 SS.reconfigure :inhaling 0.47ml/s @00:16:36.42 SS.seek 25ml @00:17:31.70 Stroke completed -- waiting for pressure to equalize ... @00:17:31.71 SS.reconfigure :exhale @00:17:31.76 SS.hold @00:17:50.32 Intake.select :close @00:17:50.47 SSV.dial :exhaust @00:17:51.23 Exhaust.select :open @00:17:51.35 SS.seek 0ml @00:18:00.14 Exhaust.select :close @00:18:00.25 SSV.dial CBV @00:18:00.28 CBV.dial SSV @00:18:01.05 Allowing 130s for 25.0ml of remaining 700.0ml @00:18:01.06 Intake.select :open @00:18:03.23 Intake @16.4psia, vacuum from 10.0 to 13.0psi @00:18:03.25 SS.reconfigure :inhaling 0.47ml/s @00:18:03.30 SS.seek 25ml @00:18:58.63 Stroke completed -- waiting for pressure to equalize ... @00:18:58.65 SS.reconfigure :exhale @00:18:58.69 SS.hold @00:19:17.30 Intake.select :close @00:19:17.46 SSV.dial :exhaust @00:19:18.23 Exhaust.select :open @00:19:18.35 SS.seek 0ml @00:19:27.17 Exhaust.select :close @00:19:27.28 SSV.dial CBV @00:19:27.31 CBV.dial SSV @00:19:28.08 Allowing 130s for 25.0ml of remaining 675.0ml @00:19:28.09 Intake.select :open @00:19:30.26 Intake @16.3psia, vacuum from 10.0 to 13.0psi @00:19:30.28 SS.reconfigure :inhaling 0.47ml/s @00:19:30.32 SS.seek 25ml @00:20:25.74 Stroke completed -- waiting for pressure to equalize ... @00:20:25.75 SS.reconfigure :exhale @00:20:25.80 SS.hold @00:20:44.38 Intake.select :close @00:20:44.54 SSV.dial :exhaust @00:20:45.31 Exhaust.select :open @00:20:45.43 SS.seek 0ml @00:20:54.29 Exhaust.select :close @00:20:54.40 SSV.dial CBV @00:20:54.44 CBV.dial SSV @00:20:55.21 Allowing 130s for 25.0ml of remaining 650.0ml @00:20:55.23 Intake.select :open @00:20:57.39 Intake @16.5psia, vacuum from 10.0 to 13.0psi @00:20:57.41 SS.reconfigure :inhaling 0.47ml/s @00:20:57.46 SS.seek 25ml @00:21:53.38 Stroke completed -- waiting for pressure to equalize ... @00:21:53.40 SS.reconfigure :exhale @00:21:53.44 SS.hold @00:22:13.85 Intake.select :close @00:22:14.01 SSV.dial :exhaust @00:22:14.80 Exhaust.select :open @00:22:14.91 SS.seek 0ml @00:22:23.73 Exhaust.select :close @00:22:24.24 SSV.dial CBV @00:22:24.27 CBV.dial SSV @00:22:25.06 Allowing 130s for 25.0ml of remaining 625.0ml @00:22:25.07 Intake.select :open @00:22:27.25 Intake @16.4psia, vacuum from 10.0 to 13.0psi @00:22:27.27 SS.reconfigure :inhaling 0.47ml/s @00:22:27.31 SS.seek 25ml @00:23:23.15 Stroke completed -- waiting for pressure to equalize ... @00:23:23.16 SS.reconfigure :exhale @00:23:23.21 SS.hold @00:23:36.80 recoverPRVreagent08 @00:23:36.81 PTV.dial :mixing @00:23:36.99 PBV.dial :waste1 @00:23:37.36 delay 30 seconds @00:23:45.77 Intake.select :close @00:23:45.93 SSV.dial :exhaust @00:23:46.69 Exhaust.select :open @00:23:46.81 SS.seek 0ml @00:23:55.55 Exhaust.select :close @00:23:55.66 SSV.dial CBV @00:23:55.68 CBV.dial SSV @00:23:56.46 Allowing 130s for 25.0ml of remaining 600.0ml @00:23:56.48 Intake.select :open @00:23:58.64 Intake @16.4psia, vacuum from 10.0 to 13.0psi @00:23:58.66 SS.reconfigure :inhaling 0.47ml/s @00:23:58.71 SS.seek 25ml @00:24:07.38 PBV.dial between PSV and waste1 @00:24:07.77 PSV.dial :air @00:24:08.24 PS.reconfigure :fast @00:24:08.29 PS.seek 2.5ml @00:24:32.31 PSV.dial :mixloop @00:24:32.78 delay 2 seconds @00:24:34.80 PSV.dial :air @00:24:35.27 delay 2 seconds @00:24:37.28 PSV.dial :mixloop @00:24:37.75 delay 2 seconds @00:24:39.77 PSV.dial :air @00:24:40.24 PS.seek 0ml @00:24:54.16 Stroke completed -- waiting for pressure to equalize ... @00:24:54.17 SS.reconfigure :exhale @00:24:54.22 SS.hold @00:25:03.75 washCycleDA #1 of 3 @00:25:03.76 PSR.select :air @00:25:03.78 PSV.dial PSR @00:25:05.11 Pulling 0.5ml of PSR.wash @00:25:05.12 PSR.select :wash @00:25:05.24 PS.reconfigure :default @00:25:05.28 PS.seek 0.5ml @00:25:11.37 delay 5 seconds @00:25:15.09 Intake.select :close @00:25:15.25 SSV.dial :exhaust @00:25:16.03 Exhaust.select :open @00:25:16.15 SS.seek 0ml @00:25:16.39 PSR.select :air @00:25:16.50 PS.seek 2ml @00:25:24.96 Exhaust.select :close @00:25:25.07 SSV.dial CBV @00:25:25.09 CBV.dial SSV @00:25:25.88 Allowing 130s for 25.0ml of remaining 575.0ml @00:25:25.90 Intake.select :open @00:25:28.06 Intake @16.5psia, vacuum from 10.0 to 13.0psi @00:25:28.08 SS.reconfigure :inhaling 0.47ml/s @00:25:28.13 SS.seek 25ml @00:25:32.72 PTV.dial :puck @00:25:33.48 PSV.dial PTV @00:25:34.25 PBV.dial :waste1 @00:25:34.68 PS.seek 1.5ml @00:25:40.72 delay 3 seconds @00:25:43.74 PBV.dial between PSV and waste1 @00:25:44.11 PS.seek 0ml @00:26:00.31 delay 2:00 @00:26:23.52 Stroke completed -- waiting for pressure to equalize ... @00:26:23.53 SS.reconfigure :exhale @00:26:23.58 SS.hold @00:26:48.37 Intake.select :close @00:26:48.53 SSV.dial :exhaust @00:26:49.32 Exhaust.select :open @00:26:49.43 SS.seek 0ml @00:26:58.50 Exhaust.select :close @00:26:59.09 SSV.dial CBV @00:26:59.11 CBV.dial SSV @00:26:59.90 Allowing 130s for 25.0ml of remaining 550.0ml @00:26:59.91 Intake.select :open @00:27:02.08 Intake @16.5psia, vacuum from 10.0 to 13.0psi @00:27:02.10 SS.reconfigure :inhaling 0.47ml/s @00:27:02.15 SS.seek 25ml @00:27:57.01 Stroke completed -- waiting for pressure to equalize ... @00:27:57.03 SS.reconfigure :exhale @00:27:57.08 SS.hold @00:28:00.33 recoverPSRreagent08 @00:28:00.34 PTV.dial :puck @00:28:00.42 PBV.dial :waste1 @00:28:00.79 delay 30 seconds @00:28:25.68 Intake.select :close @00:28:25.84 SSV.dial :exhaust @00:28:26.61 Exhaust.select :open @00:28:26.73 SS.seek 0ml @00:28:30.81 PBV.dial between PSV and waste1 @00:28:31.18 PSR.select :air @00:28:31.20 PSV.dial PSR @00:28:31.99 PS.reconfigure :fast @00:28:32.04 PS.seek 2.5ml @00:28:35.75 Exhaust.select :close @00:28:35.87 SSV.dial CBV @00:28:35.90 CBV.dial SSV @00:28:36.66 Allowing 130s for 25.0ml of remaining 525.0ml @00:28:36.67 Intake.select :open @00:28:38.84 Intake @16.5psia, vacuum from 10.0 to 13.0psi @00:28:38.86 SS.reconfigure :inhaling 0.47ml/s @00:28:38.91 SS.seek 25ml @00:28:55.99 PSV.dial PTV @00:28:56.76 delay 5 seconds @00:29:01.78 PSV.dial PSR @00:29:02.54 delay 5 seconds @00:29:07.56 PSV.dial PTV @00:29:08.32 delay 5 seconds @00:29:13.35 PSV.dial :air @00:29:14.11 PS.seek 0ml @00:29:34.00 Stroke completed -- waiting for pressure to equalize ... @00:29:34.01 SS.reconfigure :exhale @00:29:34.06 SS.hold @00:29:37.60 washCycleDA #2 of 3 @00:29:37.63 PSR.select :air @00:29:37.65 PSV.dial PSR @00:29:38.97 Pulling 0.5ml of PSR.wash @00:29:38.99 PSR.select :wash @00:29:39.11 PS.reconfigure :default @00:29:39.16 PS.seek 0.5ml @00:29:45.09 delay 5 seconds @00:29:50.11 PSR.select :air @00:29:50.23 PS.seek 2ml @00:30:02.67 Intake.select :close @00:30:02.83 SSV.dial :exhaust @00:30:03.59 Exhaust.select :open @00:30:03.71 SS.seek 0ml @00:30:06.37 PTV.dial :puck @00:30:06.45 PSV.dial PTV @00:30:07.20 PBV.dial :waste1 @00:30:08.12 PS.seek 1.5ml @00:30:12.55 Exhaust.select :close @00:30:12.66 SSV.dial CBV @00:30:12.68 CBV.dial SSV @00:30:13.47 Allowing 130s for 25.0ml of remaining 500.0ml @00:30:13.48 Intake.select :open @00:30:14.09 delay 3 seconds @00:30:15.65 Intake @16.6psia, vacuum from 10.0 to 13.0psi @00:30:15.67 SS.reconfigure :inhaling 0.47ml/s @00:30:15.72 SS.seek 25ml @00:30:17.11 PBV.dial between PSV and waste1 @00:30:17.48 PS.seek 0ml @00:30:33.59 delay 2:00 @00:31:02.63 Vacuum too high -- waiting up to 40 seconds @00:31:02.65 SS.reconfigure :exhale @00:31:02.70 SS.hold @00:31:13.96 Intake @17.8psia, vacuum from 10.0 to 13.0psi @00:31:13.98 SS.reconfigure :inhaling 0.38ml/s @00:31:14.02 SS.seek 25ml @00:31:24.95 Stroke completed -- waiting for pressure to equalize ... @00:31:24.96 SS.reconfigure :exhale @00:31:25.01 SS.hold @00:31:47.79 Intake.select :close @00:31:47.95 SSV.dial :exhaust @00:31:48.73 Exhaust.select :open @00:31:48.85 SS.seek 0ml @00:31:57.65 Exhaust.select :close @00:31:58.25 SSV.dial CBV @00:31:58.27 CBV.dial SSV @00:31:59.05 Allowing 130s for 25.0ml of remaining 475.0ml @00:31:59.06 Intake.select :open @00:32:01.23 Intake @16.6psia, vacuum from 10.0 to 13.0psi @00:32:01.25 SS.reconfigure :inhaling 0.38ml/s @00:32:01.30 SS.seek 25ml @00:32:33.62 recoverPSRreagent08 @00:32:33.63 PTV.dial :puck @00:32:33.69 PBV.dial :waste1 @00:32:34.07 delay 30 seconds @00:33:04.10 PBV.dial between PSV and waste1 @00:33:04.47 PSR.select :air @00:33:04.49 PSV.dial PSR @00:33:05.26 PS.reconfigure :fast @00:33:05.31 PS.seek 2.5ml @00:33:09.44 Stroke completed -- waiting for pressure to equalize ... @00:33:09.45 SS.reconfigure :exhale @00:33:09.50 SS.hold @00:33:29.14 PSV.dial PTV @00:33:29.93 delay 5 seconds @00:33:34.95 PSV.dial PSR @00:33:35.70 delay 5 seconds @00:33:36.04 Intake.select :close @00:33:36.20 SSV.dial :exhaust @00:33:36.99 Exhaust.select :open @00:33:37.10 SS.seek 0ml @00:33:40.71 PSV.dial PTV @00:33:41.48 delay 5 seconds @00:33:45.93 Exhaust.select :close @00:33:46.04 SSV.dial CBV @00:33:46.07 CBV.dial SSV @00:33:46.49 PSV.dial :air @00:33:46.84 Allowing 130s for 25.0ml of remaining 450.0ml @00:33:46.86 Intake.select :open @00:33:47.39 PS.seek 0ml @00:33:49.02 Intake @16.8psia, vacuum from 10.0 to 13.0psi @00:33:49.04 SS.reconfigure :inhaling 0.38ml/s @00:33:49.08 SS.seek 25ml @00:34:10.52 washCycleDA #3 of 3 @00:34:10.53 PSR.select :air @00:34:10.55 PSV.dial PSR @00:34:11.90 Pulling 0.5ml of PSR.wash @00:34:11.91 PSR.select :wash @00:34:12.02 PS.reconfigure :default @00:34:12.07 PS.seek 0.5ml @00:34:18.02 delay 5 seconds @00:34:23.04 PSR.select :air @00:34:23.16 PS.seek 2ml @00:34:39.39 PTV.dial :puck @00:34:39.47 PSV.dial PTV @00:34:40.24 PBV.dial :waste1 @00:34:40.67 PS.seek 1.5ml @00:34:46.64 delay 3 seconds @00:34:49.66 PBV.dial between PSV and waste1 @00:34:50.04 PS.seek 0ml @00:34:57.13 Stroke completed -- waiting for pressure to equalize ... @00:34:57.14 SS.reconfigure :exhale @00:34:57.19 SS.hold @00:35:06.36 delay 2:00 @00:35:28.19 Intake.select :close @00:35:28.35 SSV.dial :exhaust @00:35:29.62 Exhaust.select :open @00:35:29.74 SS.seek 0ml @00:35:38.62 Exhaust.select :close @00:35:38.73 SSV.dial CBV @00:35:38.76 CBV.dial SSV @00:35:39.53 Allowing 130s for 25.0ml of remaining 425.0ml @00:35:39.54 Intake.select :open @00:35:41.71 Intake @16.6psia, vacuum from 10.0 to 13.0psi @00:35:41.73 SS.reconfigure :inhaling 0.38ml/s @00:35:41.77 SS.seek 25ml @00:36:50.27 Stroke completed -- waiting for pressure to equalize ... @00:36:50.28 SS.reconfigure :exhale @00:36:50.33 SS.hold @00:37:06.38 recoverPSRreagent08 @00:37:06.40 PTV.dial :puck @00:37:06.47 PBV.dial :waste1 @00:37:07.33 delay 30 seconds @00:37:25.55 Intake.select :close @00:37:25.71 SSV.dial :exhaust @00:37:26.48 Exhaust.select :open @00:37:26.59 SS.seek 0ml @00:37:35.46 Exhaust.select :close @00:37:35.57 SSV.dial CBV @00:37:35.61 CBV.dial SSV @00:37:36.37 Allowing 130s for 25.0ml of remaining 400.0ml @00:37:36.38 Intake.select :open @00:37:37.35 PBV.dial between PSV and waste1 @00:37:37.70 PSR.select :air @00:37:37.72 PSV.dial PSR @00:37:38.48 PS.reconfigure :fast @00:37:38.53 PS.seek 2.5ml @00:37:38.63 Intake @16.8psia, vacuum from 10.0 to 13.0psi @00:37:38.65 SS.reconfigure :inhaling 0.38ml/s @00:37:38.69 SS.seek 25ml @00:38:02.45 PSV.dial PTV @00:38:03.22 delay 5 seconds @00:38:08.24 PSV.dial PSR @00:38:08.99 delay 5 seconds @00:38:14.01 PSV.dial PTV @00:38:14.77 delay 5 seconds @00:38:19.79 PSV.dial :air @00:38:20.57 PS.seek 0ml @00:38:28.28 Vacuum too high -- waiting up to 40 seconds @00:38:28.29 SS.reconfigure :exhale @00:38:28.35 SS.hold @00:38:38.60 Intake @17.4psia, vacuum from 10.0 to 13.0psi @00:38:38.62 SS.reconfigure :inhaling 0.31ml/s @00:38:38.67 SS.seek 25ml @00:38:43.92 Gate.power :camera,:ON @00:38:43.94 Mixing DA Substrates @00:38:43.95 PSR.select :air @00:38:43.96 PSV.dial PSR @00:38:45.39 Pulling 0.25ml of PSR.sub1 @00:38:45.41 PSR.select :sub1 @00:38:45.60 PS.reconfigure :default @00:38:45.71 PS.seek 0.25ml @00:38:49.09 delay 5 seconds @00:38:54.11 Pulling 0.25ml of PSR.sub2 @00:38:54.12 PSR.select :sub2 @00:38:54.24 PS.seek 0.5ml @00:38:57.61 delay 5 seconds @00:39:02.30 Stroke completed -- waiting for pressure to equalize ... @00:39:02.31 SS.reconfigure :exhale @00:39:02.36 SS.hold @00:39:02.66 PSR.select :air @00:39:02.86 PS.seek 2ml @00:39:19.04 PTV.dial :puck @00:39:19.12 PSV.dial PTV @00:39:19.89 PBV.dial :waste1 @00:39:20.33 PS.seek 1.5ml @00:39:26.20 delay 3 seconds @00:39:27.05 Intake.select :close @00:39:27.21 SSV.dial :exhaust @00:39:27.99 Exhaust.select :open @00:39:28.11 SS.seek 0ml @00:39:29.22 PBV.dial between PSV and waste1 @00:39:29.60 PS.seek 0ml @00:39:36.97 Exhaust.select :close @00:39:37.09 SSV.dial CBV @00:39:37.11 CBV.dial SSV @00:39:37.90 Allowing 130s for 25.0ml of remaining 375.0ml @00:39:37.91 Intake.select :open @00:39:40.57 Intake @16.8psia, vacuum from 10.0 to 13.0psi @00:39:40.59 SS.reconfigure :inhaling 0.31ml/s @00:39:40.64 SS.seek 25ml @00:39:45.67 delay 10 seconds @00:39:55.70 recoverPSRreagent08 @00:39:55.71 PTV.dial :puck @00:39:55.78 PBV.dial :waste1 @00:39:56.16 delay 30 seconds @00:40:26.18 PBV.dial between PSV and waste1 @00:40:26.56 PSR.select :air @00:40:26.58 PSV.dial PSR @00:40:27.36 PS.reconfigure :fast @00:40:27.41 PS.seek 2.5ml @00:40:51.64 PSV.dial PTV @00:40:52.42 delay 5 seconds @00:40:57.44 PSV.dial PSR @00:40:58.20 delay 5 seconds @00:41:03.22 PSV.dial PTV @00:41:04.00 delay 5 seconds @00:41:07.73 Stroke completed -- waiting for pressure to equalize ... @00:41:07.75 SS.reconfigure :exhale @00:41:07.80 SS.hold @00:41:09.07 PSV.dial :air @00:41:09.97 PS.seek 0ml @00:41:33.95 Additional air clear @00:41:33.96 PSR.select :air @00:41:33.98 PSV.dial PSR @00:41:35.34 Pulling 2ml of PSR.air @00:41:35.35 PSR.select :air @00:41:35.37 PS.reconfigure :default @00:41:35.42 PS.seek 2ml @00:41:38.97 Intake.select :close @00:41:39.13 SSV.dial :exhaust @00:41:39.89 Exhaust.select :open @00:41:40.01 SS.seek 0ml @00:41:48.86 Exhaust.select :close @00:41:48.97 SSV.dial CBV @00:41:49.01 CBV.dial SSV @00:41:49.78 Allowing 130s for 25.0ml of remaining 350.0ml @00:41:49.80 Intake.select :open @00:41:51.96 Intake @16.7psia, vacuum from 10.0 to 13.0psi @00:41:51.98 SS.reconfigure :inhaling 0.31ml/s @00:41:52.02 SS.seek 25ml @00:41:56.70 delay 5 seconds @00:42:01.72 PTV.dial :puck @00:42:01.81 PSV.dial PTV @00:42:02.59 PBV.dial :waste1 @00:42:03.02 PS.seek 1.5ml @00:42:08.95 delay 3 seconds @00:42:11.97 PBV.dial between PSV and waste1 @00:42:12.34 delay 1 second @00:42:13.36 PSV.dial :waste1 @00:42:14.45 PS.seek 0ml @00:42:30.59 Processing Puck Heater at 28.1C before imaging @00:42:30.60 PH.coast @00:42:30.71 Imaging da puck in Processing Clamp while storing it to tube 5 @00:42:31.37 SC.reconfigure :tight @00:42:31.42 SC.seek 5 @00:42:35.03 SC.configure :limp @00:42:35.24 Elbow.reconfigure :tight @00:42:35.29 Elbow.seek 5 @00:42:37.07 Forearm.reconfigure :tight @00:42:37.12 Forearm.seek 5 + 450 counts @00:42:38.31 SE.reconfigure :pick @00:42:38.37 SE.seek :beamBreak @00:42:40.89 SE.configure :default @00:42:41.05 Elbow.reconfigure :limp @00:42:41.10 Forearm.reconfigure :loose @00:42:41.15 Forearm.seek 5 @00:42:41.45 Elbow.hold @00:42:41.52 Elbow.reconfigure :tight @00:42:41.62 SE.seek 9.17 Pucks below Top Plate @00:42:42.07 Forearm.force -20 @00:42:42.11 Forearm.seek 5 + 279 counts @00:42:42.40 Forearm.reconfigure :tight @00:42:42.45 Forearm.seek 5 + 450 counts @00:42:42.65 SE.seek 10.17 Pucks below Top Plate @00:42:43.26 Forearm.reconfigure :loose @00:42:43.30 Forearm.seek :retract @00:42:44.24 Elbow.seek PC @00:42:46.74 Elbow.reconfigure :loose @00:42:46.84 Forearm.seek PC @00:42:47.82 Hand.close @00:42:48.41 PC.seek :open @00:42:53.18 Forearm.seek :retract @00:42:54.14 Elbow.reconfigure :tight @00:42:54.19 Elbow.seek 5 @00:42:57.30 Forearm.reconfigure :tight @00:42:57.35 Forearm.seek 5 + 450 counts @00:42:58.29 Hand.open @00:42:58.97 SE.seek 9.82 Pucks below Top Plate @00:42:59.25 SE.reconfigure :seal @00:42:59.30 SE.seek 8.86 Pucks below Top Plate @00:42:59.83 Lights, camera, ACTION! @00:42:59.86 PuckCamera: snap -bin=2,2 -auto /var/log/esp/da23dec0900h1000ml.tif @00:43:18.32 Stroke completed -- waiting for pressure to equalize ... @00:43:18.33 SS.reconfigure :exhale @00:43:18.39 SS.hold @00:43:19.35 Waiting >20 seconds for da to relinquish FlushPuck @00:43:21.43 SX HX9/16 (USB:23): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 18.207 seconds /var/log/esp/da23dec0900h1000ml.tif: TIFF Upload Complete @00:43:21.47 PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da23dec0900h1000ml15s.tif @00:43:37.64 SX HX9/16 (USB:23): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15 seconds /var/log/esp/midres/da23dec0900h1000ml15s.tif: TIFF Upload Complete @00:43:37.72 PuckCamera: snap -bin=2,2 20.0 /var/log/esp/midres/da23dec0900h1000ml20s.tif @00:43:54.30 Intake.select :close @00:43:54.46 SSV.dial :exhaust @00:43:55.23 Exhaust.select :open @00:43:55.35 SS.seek 0ml @00:43:58.42 SX HX9/16 (USB:23): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 20 seconds /var/log/esp/midres/da23dec0900h1000ml20s.tif: TIFF Upload Complete @00:43:58.44 Gate.power :camera,:OFF @00:43:58.46 DAfans.email "18.207 sec auto-exposure",Subject: "da23dec0900h1000ml images!" @00:43:58.87 Elbow.seek 5 @00:43:59.08 Forearm.seek 5 + 450 counts @00:43:59.16 SE.reconfigure :gently @00:43:59.23 SE.seek 9.57 Pucks below Top Plate @00:43:59.89 Elbow.reconfigure :limp @00:43:59.95 Forearm.reconfigure :loose @00:44:00.01 Forearm.seek 5 @00:44:00.30 Elbow.hold @00:44:00.36 Elbow.reconfigure :tight @00:44:00.43 SE.reconfigure :default @00:44:00.48 SE.seek 9.76 Pucks below Top Plate @00:44:00.91 Forearm.force -20 @00:44:00.97 Forearm.seek 5 + 270 counts @00:44:01.73 Forearm.reconfigure :tight @00:44:01.78 Forearm.seek 5 + 450 counts @00:44:02.22 SE.seek :down @00:44:03.51 Forearm.reconfigure :loose @00:44:03.62 Forearm.seek :retract @00:44:04.15 Exhaust.select :close @00:44:04.34 SE.stop @00:44:04.45 SSV.dial CBV @00:44:04.50 CBV.dial SSV @00:44:04.81 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @00:44:05.20 Elbow.seek FlushPuck::Garage @00:44:05.40 Allowing 130s for 25.0ml of remaining 325.0ml @00:44:05.43 Intake.select :open @00:44:07.21 Elbow.reconfigure :loose @00:44:07.44 Forearm.seek :garage @00:44:07.75 Intake @16.8psia, vacuum from 10.0 to 13.0psi @00:44:07.79 SS.reconfigure :inhaling 0.31ml/s @00:44:07.89 SS.seek 25ml @00:44:08.54 Hand.close @00:44:09.50 Forearm.seek :retract @00:44:10.52 Elbow.reconfigure :tight @00:44:10.62 Elbow.seek PC @00:44:12.02 Elbow.reconfigure :loose @00:44:12.24 Forearm.reconfigure :tight @00:44:12.34 Forearm.seek PC @00:44:13.94 PC.seek :closed @00:44:20.49 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.68 @00:44:20.64 Hand.open @00:44:21.44 Forearm.reconfigure :loose @00:44:21.53 Forearm.seek :retract @00:44:22.56 PSR.select :air @00:44:22.60 PSV.dial PSR @00:44:23.11 Pulling 0.5ml of PSR.flush @00:44:23.13 PSR.select :flush @00:44:23.30 PS.seek 0.5ml @00:44:29.35 delay 5 seconds @00:44:32.92 upsync @00:44:34.38 PSR.select :air @00:44:34.50 PS.seek 2.5ml @00:44:55.79 delay 1 second @00:44:56.81 PRV.dial :waste2,avoiding: :ab2 @00:44:58.70 PTV.dial :PRVmixing @00:45:00.04 PSV.dial :mixloop @00:45:01.08 PS.seek :empty @00:45:27.38 PSR.select :air @00:45:27.40 PSV.dial PSR @00:45:28.45 Pulling 0.5ml of PSR.flush @00:45:28.46 PSR.select :flush @00:45:28.57 PS.seek 0.5ml @00:45:34.60 delay 5 seconds @00:45:34.85 Stroke completed -- waiting for pressure to equalize ... @00:45:34.87 SS.reconfigure :exhale @00:45:34.91 SS.hold @00:45:39.62 PSR.select :air @00:45:39.80 PS.seek 2.5ml @00:46:01.10 delay 1 second @00:46:02.12 PBV.dial :waste1 @00:46:02.50 PTV.dial :mixing @00:46:03.24 PSV.dial :mixloop @00:46:04.31 PS.seek :empty @00:46:15.10 SS.reconfigure :default @00:46:15.15 SS.hold @00:46:15.22 Intake.select :close @00:46:15.38 ShallowSampler::Clogged in WCR -- Intake @17psia & Outlet @15psia won't equalize after 700.0ml @00:46:15.40 SSV.dial :exhaust @00:46:16.15 Exhaust.select :open @00:46:16.26 SS.reconfigure :exhale @00:46:16.35 SS.seek 0ml @00:46:25.56 Exhaust.select :close @00:46:25.75 Sampled 700.0ml @00:46:25.76 Drying puck before fixing for 5:00 @00:46:25.77 CBV.dial :waste2b @00:46:26.53 delay 1:00 @00:46:30.73 PSV.dial PSR @00:46:31.77 PSR.select :flush @00:46:31.90 PS.seek 1ml @00:46:42.94 PSR.select :air @00:46:43.06 PS.seek 2.5ml @00:46:59.19 PSV.dial PTV @00:46:59.96 PTV.dial :puck @00:47:00.71 PBV.dial :waste1 @00:47:00.79 PS.seek 0ml @00:47:26.55 CSV.dial :air @00:47:27.02 CS.reconfigure :fast @00:47:27.07 CS.seek 10ml @00:47:27.16 PSV.dial PSR @00:47:27.93 PSR.select :flush @00:47:28.05 PS.seek 0.5ml @00:47:33.97 PSR.select :air @00:47:34.09 PS.seek 2.5ml @00:47:51.67 Pumping wcr puck from tube 6 up to 35psi... @00:47:51.69 CBV.dial SSV @00:47:52.96 CBV.dial between CSV and waste2b @00:47:53.93 CSV.dial between air and CTV @00:47:54.26 CS.seek 6.879ml @00:47:55.38 PSV.dial PTV @00:47:56.15 PBV.dial PSV @00:47:56.72 PS.seek 0ml @00:48:02.24 CTV.dial CSV,avoiding: :air @00:48:02.26 CSV.dial CTV @00:48:03.08 CS.reconfigure :pressurize @00:48:03.14 CS.seek 0ml @00:48:16.19 CBV.dial :waste2b @00:48:16.53 CS.reconfigure :default @00:48:16.59 CS.seek 0ml @00:48:22.51 delay 1:00 @00:48:23.07 PSV.dial PBV @00:48:23.54 PS.seek 2.5ml @00:48:49.91 PSV.dial :waste1 @00:48:51.09 PS.seek 0ml @00:49:17.47 PSV.dial PSR @00:49:17.94 PSR.select :air @00:49:17.97 PS.seek 2.5ml @00:49:22.54 CTV.dial :air,avoiding: :intake @00:49:23.88 Fix #1 of 2 (with RNAlater) for 2:30 @00:49:23.89 CSV.dial CSR @00:49:24.33 Pulling 2ml of CSR.RNAlater @00:49:24.34 CSR.select :RNAlater @00:49:24.46 CS.seek 2ml @00:49:30.45 delay 5 seconds @00:49:35.47 CSR.select :air @00:49:35.60 CS.reconfigure :fast @00:49:35.65 CS.seek 10ml @00:49:44.38 PSV.dial PTV @00:49:45.11 PBV.dial PSV @00:49:45.20 PS.seek 0ml @00:49:55.36 Pumping wcr puck from tube 6 up to 35psi... @00:49:55.38 CBV.dial SSV @00:49:56.64 CBV.dial between CSV and waste2b @00:49:57.62 CSV.dial between air and CTV @00:49:58.22 CS.seek 6.864ml @00:50:06.30 CTV.dial CSV,avoiding: :air @00:50:06.32 CSV.dial CTV @00:50:07.72 CS.reconfigure :pressurize @00:50:07.77 CS.seek 0ml @00:50:11.57 PSV.dial PBV @00:50:12.04 PS.seek 2.5ml @00:50:20.44 delay 2:30 @00:50:38.41 PSV.dial :waste1 @00:50:39.17 PS.seek 0ml @00:51:05.47 PSV.dial PSR @00:51:05.94 PSR.select :flush @00:51:06.06 PS.seek 1ml @00:51:17.41 PSR.select :air @00:51:17.53 PS.seek 2.5ml @00:51:33.72 PSV.dial PTV @00:51:34.45 PTV.dial :puck @00:51:34.64 PBV.dial :waste1 @00:51:35.21 PS.seek 0ml @00:52:01.50 PSV.dial PSR @00:52:02.68 PSR.select :flush @00:52:02.81 PS.seek 0.5ml @00:52:08.72 PSR.select :air @00:52:08.84 PS.seek 2.5ml @00:52:30.16 PSV.dial PTV @00:52:30.92 PBV.dial PSV @00:52:31.49 PS.seek 0ml @00:52:50.47 CBV.dial :waste2b @00:52:50.81 CS.reconfigure :default @00:52:50.86 CS.seek 0ml @00:52:57.23 delay 1:00 @00:52:57.84 PSV.dial PBV @00:52:58.32 PS.seek 2.5ml @00:53:24.69 PSV.dial :waste1 @00:53:25.55 PS.seek 0ml @00:53:51.94 PSV.dial PSR @00:53:52.39 PSR.select :air @00:53:52.41 PS.seek 2.5ml @00:53:57.25 CTV.dial :air,avoiding: :intake @00:53:58.58 Fix #2 of 2 (with RNAlater) for 2:30 @00:53:58.59 CSV.dial CSR @00:53:59.03 Pulling 2ml of CSR.RNAlater @00:53:59.04 CSR.select :RNAlater @00:53:59.15 CS.seek 2ml @00:54:05.04 delay 5 seconds @00:54:10.06 CSR.select :air @00:54:10.18 CS.reconfigure :fast @00:54:10.23 CS.seek 10ml @00:54:18.68 PSV.dial PTV @00:54:19.42 PBV.dial PSV @00:54:19.61 PS.seek 0ml @00:54:29.98 Pumping wcr puck from tube 6 up to 35psi... @00:54:30.00 CBV.dial SSV @00:54:31.25 CBV.dial between CSV and waste2b @00:54:32.22 CSV.dial between air and CTV @00:54:32.83 CS.seek 6.835ml @00:54:40.98 CTV.dial CSV,avoiding: :air @00:54:41.01 CSV.dial CTV @00:54:42.40 CS.reconfigure :pressurize @00:54:42.46 CS.seek 0ml @00:54:45.97 PSV.dial PBV @00:54:46.90 PS.seek 2.5ml @00:54:59.62 delay 2:30 @00:55:13.31 PSV.dial :waste1 @00:55:14.07 PS.seek 0ml @00:55:40.59 Garaging FlushPuck in Processing Clamp @00:55:40.66 Elbow.reconfigure :tight @00:55:40.71 Elbow.seek PC @00:55:40.83 Elbow.reconfigure :loose @00:55:40.94 Forearm.seek PC @00:55:41.91 Hand.close @00:55:42.52 PC.seek :open @00:55:47.27 Forearm.seek :retract @00:55:48.29 Elbow.reconfigure :tight @00:55:48.34 Elbow.seek :garage @00:55:49.70 Elbow.reconfigure :loose @00:55:49.80 Forearm.reconfigure :tight @00:55:49.85 Forearm.seek :garage @00:55:50.91 Hand.open @00:55:51.58 Forearm.reconfigure :loose @00:55:51.63 Forearm.seek :retract @00:57:29.65 CBV.dial :waste2b @00:57:29.98 CS.reconfigure :default @00:57:30.03 CS.seek 0ml @00:57:31.76 delay 1:00 @00:58:31.78 CTV.dial :air,avoiding: :intake @00:58:33.16 Storing wcr puck in Collection Clamp to tube 5 @00:58:33.56 Elbow.reconfigure :tight @00:58:33.61 Elbow.seek 5 @00:58:35.48 Forearm.reconfigure :tight @00:58:35.53 Forearm.seek 5 + 450 counts @00:58:36.96 SE.reconfigure :pick @00:58:37.04 SE.seek :beamBreak @00:58:39.52 SE.configure :default @00:58:39.69 Elbow.reconfigure :limp @00:58:39.74 Forearm.reconfigure :loose @00:58:39.79 Forearm.seek 5 @00:58:40.03 Elbow.hold @00:58:40.10 Elbow.reconfigure :tight @00:58:40.21 SE.seek 10.14 Pucks below Top Plate @00:58:40.64 Forearm.force -20 @00:58:40.69 Forearm.seek 5 + 276 counts @00:58:40.98 Forearm.reconfigure :tight @00:58:41.03 Forearm.seek 5 + 450 counts @00:58:41.27 SE.seek 11.14 Pucks below Top Plate @00:58:41.93 Forearm.reconfigure :loose @00:58:41.98 Forearm.seek :retract @00:58:42.90 Elbow.seek CC @00:58:44.54 Elbow.reconfigure :loose @00:58:44.65 Forearm.seek CC @00:58:45.61 Hand.close @00:58:46.27 CC.seek :open @00:58:51.02 Forearm.seek :retract @00:58:51.97 Elbow.reconfigure :tight @00:58:52.02 Elbow.seek 5 @00:58:53.78 Elbow.reconfigure :limp @00:58:54.21 Forearm.seek 5 @00:58:55.27 Elbow.hold @00:58:55.34 Elbow.reconfigure :tight @00:58:55.39 Hand.open @00:58:56.14 Forearm.force -20 @00:58:56.18 Forearm.seek 5 + 318 counts @00:58:56.48 Forearm.reconfigure :tight @00:58:56.54 Forearm.seek 5 + 450 counts @00:58:56.79 SE.seek :down @00:58:57.92 Forearm.reconfigure :loose @00:58:57.97 Forearm.seek :retract @00:58:58.73 SE.stop @00:58:58.96
Starting finalPurge @00:58:58.97 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @00:58:59.16 Elbow.seek FlushPuck::Garage @00:59:01.01 Elbow.reconfigure :loose @00:59:01.12 Forearm.seek :garage @00:59:02.06 Hand.close @00:59:02.76 Forearm.seek :retract @00:59:03.71 Elbow.reconfigure :tight @00:59:03.75 Elbow.seek CC @00:59:06.72 Elbow.reconfigure :loose @00:59:06.82 Forearm.reconfigure :tight @00:59:06.87 Forearm.seek CC @00:59:08.16 CC.seek :closed @00:59:12.67 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.32 @00:59:12.73 Hand.open @00:59:13.45 Forearm.reconfigure :loose @00:59:13.50 Forearm.seek :retract @00:59:14.47 Flushing the Collection Valve::Manifold @00:59:14.60 CSV.dial CSR @00:59:15.04 Pulling 4.999ml of CSR.flush CSR.select :flush @00:59:15.16 CS.seek 5ml @00:59:29.25 delay 5 seconds @00:59:34.27 CSR.select :air @00:59:34.44 CS.reconfigure :fast @00:59:34.49 CS.seek 7.5ml @00:59:41.28 CSV.dial :waste @00:59:42.59 CS.reconfigure :default @00:59:42.63 CS.seek 0ml @01:00:02.72 Flushing lower manifold's waste1 port @01:00:02.84 CSV.dial :flush @01:00:03.31 CS.reconfigure :fast @01:00:03.35 CS.seek 5ml @01:00:16.28 delay 5 seconds @01:00:21.30 CSV.dial :air @01:00:21.82 CS.seek 7ml @01:00:27.47 CSV.dial CTV @01:00:27.50
CTV.dial CSV @01:00:28.88 CBV.dial :waste1 @01:00:29.65 CS.seek 0ml @01:00:46.53 Flushing lower manifold's waste2 port @01:00:46.65 CSV.dial :flush @01:00:47.40 CS.seek 5ml @01:01:00.38 delay 5 seconds @01:01:05.40 CSV.dial :air @01:01:05.91 CS.seek 6.999ml @01:01:11.60 CSV.dial CTV @01:01:11.62
CTV.dial CSV @01:01:12.10 CBV.dial :waste2 @01:01:13.15 CS.seek 0ml @01:01:29.81 Flushing lower manifold's waste2b port @01:01:29.93 CSV.dial :flush @01:01:30.66 CS.seek 5ml @01:01:44.06 delay 5 seconds @01:01:49.08 CSV.dial :air @01:01:49.61 CS.seek 7ml @01:01:55.13 CSV.dial CTV @01:01:55.15
CTV.dial CSV @01:01:55.60 CBV.dial :waste2b @01:01:56.65 CS.seek 0ml @01:02:13.35 Disinfecting external Intake with kill @01:02:13.47 CSV.dial CSR @01:02:14.03 Pulling 8ml of CSR.kill @01:02:14.04 CSR.select :kill @01:02:14.16 CS.seek 8ml @01:02:34.13 delay 5 seconds @01:02:39.14 CSR.select :air @01:02:39.26 CS.seek 10ml @01:02:44.79 CSV.dial CBV @01:02:44.81
CBV.dial CSV @01:02:46.00 CTV.dial :intake @01:02:46.78 Intake.select :open @01:02:46.89 CS.seek 2ml @01:03:05.87 Intake.select :close @01:03:05.98 CTV.dial :air @01:03:06.76 CS.reconfigure :default @01:03:06.80 CS.seek 0ml @01:03:12.75 CSV.dial CSR @01:03:13.75 Pulling 5ml of CSR.flush @01:03:13.76 CSR.select :flush @01:03:13.87 CS.seek 5ml @01:03:27.47 delay 5 seconds @01:03:32.49 CSR.select :air @01:03:32.60 CS.seek 10ml @01:03:46.28 CSV.dial CBV @01:03:47.30 CS.seek 0ml @01:04:13.75 Garaging FlushPuck in Collection Clamp @01:04:13.82 Elbow.reconfigure :tight @01:04:13.87 Elbow.seek CC @01:04:14.13 Elbow.reconfigure :loose @01:04:14.23 Forearm.seek CC @01:04:15.26 Hand.close @01:04:15.89 CC.seek :open @01:04:20.52 Forearm.seek :retract @01:04:21.48 Elbow.reconfigure :tight @01:04:21.53 Elbow.seek :garage @01:04:24.48 Elbow.reconfigure :loose @01:04:24.58 Forearm.reconfigure :tight @01:04:24.63 Forearm.seek :garage @01:04:26.09 Hand.open @01:04:26.83 Forearm.reconfigure :loose @01:04:26.87 Forearm.seek :retract @01:04:27.83 Completed [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] begun 22:16:32 @01:04:27.88 Asleep after completing DA_WCR_120823b assays @01:04:27.90 GoodNews.email "{1 => 33,\n 2 => 33,\n 3 => 31,\n 4 => 33,\n 5 => 12,\n 6 => 21,\n 7 => 31}\nCTD data unavailable\nCan@00:55:49, 29% flow, 17.3C, 33% humidity, 15.1psia, 13.695V, 0.423A, 182.385Ah, 5.80W\nSampled 700.0ml",Subject: "Asleep after completing DA_WCR_120823b assays" @01:04:32.64 upsync + ESPwhich DA_WCR_121123.rb + fn=DA_WCR_121123.rb + IFS=: + set /home/esp/esp2/mission . + fullPath=/home/esp/esp2/mission/DA_WCR_121123.rb + [ -f /home/esp/esp2/mission/DA_WCR_121123.rb ] + realpath /home/esp/esp2/mission/DA_WCR_121123.rb + return 0 + scriptPath=/home/esp/esp2local/mission/DA_WCR_121123.rb + exec ruby /home/esp/esp2/lib/esp.rb /home/esp/esp2local/mission/DA_WCR_121123.rb @13:28:24.77UTC11-Dec-23
ESP2 v3.42 I2C Gateway v3.20 for core bus @13:28:27.54 Configuration for short puck jake 1/11/21 by brent@mbari.org /home/esp/esp2/type/shallow/jake/configure.rb @13:28:31.33 Polling Can every 10:00 @13:28:31.48 Cleanly exited at 13:24:42UTC11-Dec-23 @13:28:31.53 Loading /home/esp/esp2local/mission/DA_WCR_121123.rb mission @13:28:31.59 Preparing to sleep until + 00:00:05 @13:28:31.60 GoodNews.email "Starting DA_WCR_121123 w/pucks in tube 6\n{1 => 33,\n 2 => 33,\n 3 => 33,\n 4 => 33,\n 5 => 12,\n 6 => 21,\n 7 => 31}\nCTD data unavailable\nCan@13:28:31, 31% flow, 16.5C, 32% humidity, 15.1psia, 13.720V, 0.302A, 198.561Ah, 4.15W",Subject: "DA_WCR_121123 begun" @13:28:31.64
Asleep til + 00:00:05 @13:28:31.69 Continuing at 13:28 @13:28:31.93 Gate.power :core,:OFF @13:28:31.94 Gate.power :raw,:OFF @13:28:31.95 Shutdown @13:28:36.19 GoodNews.email "Then, in tube 6: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_121123.rb:14\n{1 => 33,\n 2 => 33,\n 3 => 33,\n 4 => 33,\n 5 => 12,\n 6 => 21,\n 7 => 31}\nCTD data unavailable\nCan@13:28:31, 31% flow, 16.5C, 32% humidity, 15.1psia, 13.730V, 0.296A, 198.561Ah, 4.07W",Subject: "Asleep until 13:28" @13:28:36.57
Powering core bus @13:28:37.57 upsync @13:28:41.10 upsync @13:28:42.33
Gate.power :raw,:ON @13:28:43.02 CS.configure :default @13:28:43.07 PS.configure :default @13:28:43.12 SS.configure :default @13:28:43.21 CC.configure :default @13:28:43.26 PC.configure :default @13:28:43.33 Forearm.configure :loose @13:28:43.38 Elbow.configure :loose @13:28:43.45 SC.configure :loose @13:28:43.49 SE.configure :default @13:28:43.54 CH.configure :default @13:28:43.58 PH.configure :default @13:28:43.62 All dwarf controllers running firmware version 3.85 @13:28:43.73 Valve.reset @13:28:43.96 Intake.select :close @13:28:44.01 Exhaust.select :close @13:28:44.05 CTV.dial :air @13:28:44.14 PSV.dial :air @13:28:44.28
Forearm.home.seek :retract @13:28:44.29 CSV.dial :waste1 @13:28:44.78 SSV.dial :spit,avoiding: :exhaust @13:28:45.01 Hand.close @13:28:45.06
Elbow.reconfigure :loose @13:28:45.14 Elbow.jog 1500 @13:28:45.38 PS.home.seek 0ml @13:28:45.67
Elbow.reconfigure :loose @13:28:45.72 CS.reconfigure :fast @13:28:45.74 SS.home.seek 0ml @13:28:45.86
Elbow.home.seek :clear @13:28:45.92 CS.home.seek 0ml @13:28:50.43
SE.home.seek :down @13:28:51.04 SC.reconfigure :tight @13:28:51.09 SC.home.seek :initial @13:28:53.63 PS.stop @13:29:19.38
SC.configure :limp @13:29:19.43 Forearm.seek :retract @13:29:19.50 Elbow.seek :hammer @13:29:20.54 Forearm.reconfigure :tight @13:29:20.59 Forearm.seek retracted @13:29:21.25 PC.home.seek :open @13:29:21.27
CC.home.seek :open @13:29:24.64 Phase [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] @13:29:24.66 Notice.email "At 13:29:24 began in tube 6: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_121123.rb:14\n{1 => 33,\n 2 => 33,\n 3 => 33,\n 4 => 33,\n 5 => 12,\n 6 => 21,\n 7 => 31}\nCTD data unavailable\nCan@13:29:24, 31% flow, 16.5C, 32% humidity, 15.1psia, 13.695V, 0.290A, 198.566Ah, 3.98W",Subject: "Started DA_WCR_121123 phase initialPurge; da [1000, 1000] #da and wsr are run simultanelously" @13:29:24.67
Starting initialPurge @13:29:24.70 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @13:29:24.77 Hand.open @13:29:25.51 Elbow.reconfigure :tight @13:29:25.56 Elbow.seek FlushPuck::Garage @13:29:27.43 Elbow.reconfigure :loose @13:29:27.53 Forearm.reconfigure :loose @13:29:27.58 Forearm.seek :garage @13:29:28.58 Hand.close @13:29:29.12 upsync @13:29:29.40
Forearm.seek :retract @13:29:30.39 Elbow.reconfigure :tight @13:29:30.44 Elbow.seek CC @13:29:33.43 Elbow.reconfigure :loose @13:29:33.54 Forearm.reconfigure :tight @13:29:33.59 Forearm.seek CC @13:29:34.83 CC.seek :closed @13:29:39.38 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.41 @13:29:39.45 Hand.open @13:29:40.07 Forearm.reconfigure :loose @13:29:40.13 Forearm.seek :retract @13:29:41.08 Flushing the Collection Valve::Manifold @13:29:41.20 CSV.dial CSR @13:29:42.49 Pulling 5ml of CSR.flush @13:29:42.50 CSR.select :flush @13:29:42.62 CS.reconfigure :default @13:29:42.67 CS.seek 5ml @13:29:56.90 delay 5 seconds @13:30:01.92 CSR.select :air @13:30:02.09 CS.reconfigure :fast @13:30:02.14 CS.seek 7.5ml @13:30:08.96 CSV.dial :waste @13:30:10.27 CS.reconfigure :default @13:30:10.31 CS.seek 0ml @13:30:30.31 Purging external Intake @13:30:30.43 CSV.dial :flush @13:30:30.89 CS.reconfigure :fast @13:30:30.94 CS.seek 10ml @13:30:55.59 delay 5 seconds @13:31:00.61 CSV.dial CBV @13:31:00.64
CBV.dial CSV @13:31:01.40 CTV.dial :intake @13:31:02.19 Intake.select :open @13:31:02.31 CS.seek 0ml @13:31:26.02 Intake.select :close @13:31:26.13 CTV.dial :air @13:31:26.92 Flushing upper manifold @13:31:27.04 CSV.dial :flush @13:31:27.91 CS.seek 5ml @13:31:40.65 delay 5 seconds @13:31:45.67 CSV.dial :air @13:31:46.19 CS.seek 7ml @13:31:51.71 CSV.dial CBV @13:31:51.73
CBV.dial CSV @13:31:52.77 CTV.dial :air @13:31:52.86 CS.seek 0ml @13:32:09.55 delay 7 seconds @13:32:16.58 CS.seek 3ml @13:32:24.68 CSV.dial :waste @13:32:25.14 CS.seek 0ml @13:32:32.95 CSV.dial :flush @13:32:33.40 CS.seek 3ml @13:32:41.43 delay 5 seconds @13:32:46.45 CSV.dial :air @13:32:46.94 CS.seek 5ml @13:32:52.68 CSV.dial :air @13:32:52.76 CS.seek 0ml @13:33:04.96 delay 7 seconds @13:33:11.99 CS.seek 3ml @13:33:20.05 CSV.dial :waste @13:33:20.79 CS.seek 0ml @13:33:28.52 Garaging FlushPuck in Collection Clamp @13:33:28.59 Elbow.reconfigure :tight @13:33:28.64 Elbow.seek CC @13:33:28.94 Elbow.reconfigure :loose @13:33:29.04 Forearm.seek CC @13:33:30.07 Hand.close @13:33:30.64 CC.seek :open @13:33:35.66 Forearm.seek :retract @13:33:36.68 Elbow.reconfigure :tight @13:33:36.73 Elbow.seek :garage @13:33:39.60 Elbow.reconfigure :loose @13:33:39.70 Forearm.reconfigure :tight @13:33:39.75 Forearm.seek :garage @13:33:40.82 Hand.open @13:33:41.44 Forearm.reconfigure :loose @13:33:41.49 Forearm.seek :retract @13:33:42.48 da sampling at most 1000ml, wcr at most 1000ml @13:33:42.52 DAfans.email "Started da in tube 6 at 13:33:42UTC11-Dec-23\n{1 => 33,\n 2 => 33,\n 3 => 33,\n 4 => 33,\n 5 => 12,\n 6 => 21,\n 7 => 31}\nCTD data unavailable\nCan@13:29:24, 31% flow, 16.5C, 32% humidity, 15.1psia, 13.695V, 0.290A, 198.566Ah, 3.98W",Subject: "da sampling at most 1000ml, wcr at most 1000ml" @13:33:42.56
Loading sh1 puck from tube 6 into Collection Clamp @13:33:42.81 SC.reconfigure :tight @13:33:42.87 SC.seek 6 @13:33:47.02 upsync @13:33:49.62
SC.configure :limp @13:33:49.85 Elbow.reconfigure :tight @13:33:49.90 Elbow.seek 6 @13:33:51.83 Forearm.reconfigure :tight @13:33:51.88 Forearm.seek 6 + 450 counts @13:33:52.97 SE.reconfigure :pick @13:33:53.03 SE.seek :beamBreak @13:33:54.84 SE.configure :default @13:33:55.01 Elbow.seek 6 @13:33:55.20 Forearm.seek 6 + 300 counts @13:33:55.40 Forearm.reconfigure :loose @13:33:55.45 Hand.close @13:33:56.02 SE.seek :down @13:33:56.25
Forearm.seek :retract @13:33:57.53 SE.stop @13:33:57.62
Elbow.seek CC @13:33:59.21 Elbow.reconfigure :loose @13:33:59.31 Forearm.reconfigure :tight @13:33:59.36 Forearm.seek CC @13:34:00.57 CC.seek :closed @13:34:05.09 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.37 @13:34:05.10 Hand.open @13:34:05.82 Forearm.reconfigure :loose @13:34:05.87 Forearm.seek :retract @13:34:06.82 collectSample 1000ml w/bubble point of 24psi @13:34:06.84 Priming 7ml @13:34:06.85 Intake.select :close @13:34:06.86 Exhaust.select :close @13:34:06.87 SS.hold @13:34:07.08 Calibrating pressure and temperature sensors @13:34:07.11 CTV.dial :air @13:34:07.20 SSV.dial :spit,avoiding: :exhaust @13:34:07.27 SS.reconfigure :fast @13:34:07.32 SS.seek 5ml @13:34:10.19 SSV.dial CBV @13:34:10.23
CBV.dial SSV @13:34:11.55 delay 3.8 seconds @13:34:15.48 Sensor limits: Intake max 100.6psia; Outlet max 94.0psia @13:34:15.49 SSV.dial :spit @13:34:16.82 SS.seek 0ml @13:34:19.63 SSV.dial CBV @13:34:20.94 CTV.dial :intake @13:34:21.74 SSV.dial CBV @13:34:21.77
CBV.dial SSV @13:34:21.97 Allowing 40s for 7.0ml of remaining 7.0ml @13:34:21.98 Intake.select :open @13:34:24.15 Intake @14.2psia, vacuum from 8.7 to 11.7psi @13:34:24.17 SS.reconfigure :inhaling 2.20ml/s @13:34:24.22 SS.seek 7ml @13:34:30.98 Stroke completed -- waiting for pressure to equalize ... @13:34:30.99 SS.reconfigure :exhale @13:34:31.04 SS.hold @13:34:36.85 Intake.select :close @13:34:37.01 SSV.dial :exhaust @13:34:37.77 Exhaust.select :open @13:34:37.88 SS.seek 0ml @13:34:41.84 Exhaust.select :close @13:34:41.94 Sampling 1000.0ml @13:34:41.95 Intake.select :close @13:34:41.97 Exhaust.select :close @13:34:41.98 SS.reconfigure :default @13:34:42.02 SS.hold @13:34:42.23 CTV.dial :intake @13:34:42.40 SSV.dial CBV @13:34:42.42
CBV.dial SSV @13:34:43.22 Allowing 130s for 25.0ml of remaining 1000.0ml @13:34:43.23 Intake.select :open @13:34:45.40 Intake @15.1psia, vacuum from 9.6 to 12.6psi @13:34:45.41 SS.reconfigure :inhaling 2.20ml/s @13:34:45.46 SS.seek 25ml @13:35:00.70 Stroke completed -- waiting for pressure to equalize ... @13:35:00.71 SS.reconfigure :exhale @13:35:00.76 SS.hold @13:35:27.71 Intake.select :close @13:35:27.87 SSV.dial :exhaust @13:35:28.63 Exhaust.select :open @13:35:28.75 SS.seek 0ml @13:35:37.76 Exhaust.select :close @13:35:37.87 SSV.dial CBV @13:35:37.91
CBV.dial SSV @13:35:38.66 Allowing 130s for 25.0ml of remaining 975.0ml @13:35:38.67 Intake.select :open @13:35:40.83 Intake @15.5psia, vacuum from 10.0 to 13.0psi @13:35:40.84 SS.reconfigure :inhaling 2.20ml/s @13:35:40.89 SS.seek 25ml @13:35:55.67 Stroke completed -- waiting for pressure to equalize ... @13:35:55.68 SS.reconfigure :exhale @13:35:55.73 SS.hold @13:36:26.27 Intake.select :close @13:36:26.43 SSV.dial :exhaust @13:36:27.19 Exhaust.select :open @13:36:27.30 SS.seek 0ml @13:36:36.26 Exhaust.select :close @13:36:36.37 SSV.dial CBV @13:36:36.39
CBV.dial SSV @13:36:37.20 Allowing 130s for 25.0ml of remaining 950.0ml @13:36:37.21 Intake.select :open @13:36:39.38 Intake @15.8psia, vacuum from 10.0 to 13.0psi @13:36:39.39 SS.reconfigure :inhaling 2.20ml/s @13:36:39.44 SS.seek 25ml @13:36:51.65 Vacuum too high -- waiting up to 40 seconds @13:36:51.66 SS.reconfigure :exhale @13:36:51.71 SS.hold @13:36:59.01 Intake @18.8psia, vacuum from 10.0 to 13.0psi @13:36:59.03 SS.reconfigure :inhaling 1.76ml/s @13:36:59.07 SS.seek 25ml @13:37:03.15 Stroke completed -- waiting for pressure to equalize ... @13:37:03.16 SS.reconfigure :exhale @13:37:03.21 SS.hold @13:37:23.62 Intake.select :close @13:37:23.78 SSV.dial :exhaust @13:37:24.55 Exhaust.select :open @13:37:24.67 SS.seek 0ml @13:37:33.47 Exhaust.select :close @13:37:33.58 SSV.dial CBV @13:37:33.62
CBV.dial SSV @13:37:34.36 Allowing 130s for 25.0ml of remaining 925.0ml @13:37:34.37 Intake.select :open @13:37:36.53 Intake @15.8psia, vacuum from 10.0 to 13.0psi @13:37:36.55 SS.reconfigure :inhaling 1.76ml/s @13:37:36.60 SS.seek 25ml @13:37:53.98 Stroke completed -- waiting for pressure to equalize ... @13:37:53.99 SS.reconfigure :exhale @13:37:54.04 SS.hold @13:38:21.02 Intake.select :close @13:38:21.18 SSV.dial :exhaust @13:38:21.93 Exhaust.select :open @13:38:22.05 SS.seek 0ml @13:38:30.84 Exhaust.select :close @13:38:30.95 SSV.dial CBV @13:38:30.97
CBV.dial SSV @13:38:31.75 Allowing 130s for 25.0ml of remaining 900.0ml @13:38:31.76 Intake.select :open @13:38:33.92 Intake @15.8psia, vacuum from 10.0 to 13.0psi @13:38:33.93 SS.reconfigure :inhaling 1.76ml/s @13:38:33.98 SS.seek 25ml @13:38:51.46 Stroke completed -- waiting for pressure to equalize ... @13:38:51.47 SS.reconfigure :exhale @13:38:51.52 SS.hold @13:39:16.28 Intake.select :close @13:39:16.48 SSV.dial :exhaust @13:39:17.25 Exhaust.select :open @13:39:17.36 SS.seek 0ml @13:39:26.12 Exhaust.select :close @13:39:26.23 SSV.dial CBV @13:39:26.25
CBV.dial SSV @13:39:27.03 Allowing 130s for 25.0ml of remaining 875.0ml @13:39:27.04 Intake.select :open @13:39:29.20 Intake @15.9psia, vacuum from 10.0 to 13.0psi @13:39:29.21 SS.reconfigure :inhaling 1.76ml/s @13:39:29.26 SS.seek 25ml @13:39:46.66 Stroke completed -- waiting for pressure to equalize ... @13:39:46.67 SS.reconfigure :exhale @13:39:46.72 SS.hold @13:40:09.62 Intake.select :close @13:40:09.77 SSV.dial :exhaust @13:40:10.53 Exhaust.select :open @13:40:10.64 SS.seek 0ml @13:40:19.42 Exhaust.select :close @13:40:19.53 SSV.dial CBV @13:40:19.55
CBV.dial SSV @13:40:20.34 Allowing 130s for 25.0ml of remaining 850.0ml @13:40:20.35 Intake.select :open @13:40:22.52 Intake @15.9psia, vacuum from 10.0 to 13.0psi @13:40:22.53 SS.reconfigure :inhaling 1.76ml/s @13:40:22.59 SS.seek 25ml @13:40:39.73 Stroke completed -- waiting for pressure to equalize ... @13:40:39.74 SS.reconfigure :exhale @13:40:39.78 SS.hold @13:41:00.30 Intake.select :close @13:41:00.45 SSV.dial :exhaust @13:41:01.22 Exhaust.select :open @13:41:01.34 SS.seek 0ml @13:41:10.12 Exhaust.select :close @13:41:10.23 SSV.dial CBV @13:41:10.25
CBV.dial SSV @13:41:11.02 Allowing 130s for 25.0ml of remaining 825.0ml @13:41:11.04 Intake.select :open @13:41:13.20 Intake @16.0psia, vacuum from 10.0 to 13.0psi @13:41:13.21 SS.reconfigure :inhaling 1.76ml/s @13:41:13.26 SS.seek 25ml @13:41:30.60 Stroke completed -- waiting for pressure to equalize ... @13:41:30.61 SS.reconfigure :exhale @13:41:30.66 SS.hold @13:41:51.08 Intake.select :close @13:41:51.24 SSV.dial :exhaust @13:41:52.02 Exhaust.select :open @13:41:52.13 SS.seek 0ml @13:42:00.91 Exhaust.select :close @13:42:01.02 SSV.dial CBV @13:42:01.04
CBV.dial SSV @13:42:01.84 Allowing 130s for 25.0ml of remaining 800.0ml @13:42:01.85 Intake.select :open @13:42:04.01 Intake @15.9psia, vacuum from 10.0 to 13.0psi @13:42:04.02 SS.reconfigure :inhaling 1.76ml/s @13:42:04.07 SS.seek 25ml @13:42:21.50 Stroke completed -- waiting for pressure to equalize ... @13:42:21.52 SS.reconfigure :exhale @13:42:21.56 SS.hold @13:42:40.42 Intake.select :close @13:42:40.58 SSV.dial :exhaust @13:42:41.34 Exhaust.select :open @13:42:41.45 SS.seek 0ml @13:42:50.46 Exhaust.select :close @13:42:50.57 SSV.dial CBV @13:42:50.59
CBV.dial SSV @13:42:51.38 Allowing 130s for 25.0ml of remaining 775.0ml @13:42:51.39 Intake.select :open @13:42:53.56 Intake @16.0psia, vacuum from 10.0 to 13.0psi @13:42:53.58 SS.reconfigure :inhaling 1.76ml/s @13:42:53.63 SS.seek 25ml @13:43:10.54 Stroke completed -- waiting for pressure to equalize ... @13:43:10.55 SS.reconfigure :exhale @13:43:10.59 SS.hold @13:43:28.94 Intake.select :close @13:43:29.10 SSV.dial :exhaust @13:43:29.88 Exhaust.select :open @13:43:30.00 SS.seek 0ml @13:43:38.96 Exhaust.select :close @13:43:39.07 SSV.dial CBV @13:43:39.09
CBV.dial SSV @13:43:39.87 Allowing 130s for 25.0ml of remaining 750.0ml @13:43:39.88 Intake.select :open @13:43:42.04 Intake @15.9psia, vacuum from 10.0 to 13.0psi @13:43:42.05 SS.reconfigure :inhaling 1.76ml/s @13:43:42.10 SS.seek 25ml @13:43:59.30 Stroke completed -- waiting for pressure to equalize ... @13:43:59.31 SS.reconfigure :exhale @13:43:59.36 SS.hold @13:44:17.71 Intake.select :close @13:44:17.87 SSV.dial :exhaust @13:44:18.70 Exhaust.select :open @13:44:18.82 SS.seek 0ml @13:44:27.44 Exhaust.select :close @13:44:27.56 SSV.dial CBV @13:44:27.59
CBV.dial SSV @13:44:28.36 Allowing 130s for 25.0ml of remaining 725.0ml @13:44:28.38 Intake.select :open @13:44:30.54 Intake @16.0psia, vacuum from 10.0 to 13.0psi @13:44:30.55 SS.reconfigure :inhaling 1.76ml/s @13:44:30.61 SS.seek 25ml @13:44:47.89 Stroke completed -- waiting for pressure to equalize ... @13:44:47.90 SS.reconfigure :exhale @13:44:47.95 SS.hold @13:45:06.31 Intake.select :close @13:45:06.46 SSV.dial :exhaust @13:45:07.22 Exhaust.select :open @13:45:07.34 SS.seek 0ml @13:45:16.05 Exhaust.select :close @13:45:16.16 SSV.dial CBV @13:45:16.18
CBV.dial SSV @13:45:16.97 Allowing 130s for 25.0ml of remaining 700.0ml @13:45:16.98 Intake.select :open @13:45:19.15 Intake @16.0psia, vacuum from 10.0 to 13.0psi @13:45:19.16 SS.reconfigure :inhaling 1.76ml/s @13:45:19.64 SS.seek 25ml @13:45:36.94 Stroke completed -- waiting for pressure to equalize ... @13:45:36.95 SS.reconfigure :exhale @13:45:37.00 SS.hold @13:45:55.37 Intake.select :close @13:45:55.52 SSV.dial :exhaust @13:45:56.28 Exhaust.select :open @13:45:56.40 SS.seek 0ml @13:46:05.41 Exhaust.select :close @13:46:05.52 SSV.dial CBV @13:46:05.54
CBV.dial SSV @13:46:06.30 Allowing 130s for 25.0ml of remaining 675.0ml @13:46:06.31 Intake.select :open @13:46:08.48 Intake @15.9psia, vacuum from 10.0 to 13.0psi @13:46:08.49 SS.reconfigure :inhaling 1.76ml/s @13:46:08.54 SS.seek 25ml @13:46:25.34 Stroke completed -- waiting for pressure to equalize ... @13:46:25.35 SS.reconfigure :exhale @13:46:25.40 SS.hold @13:46:43.98 Intake.select :close @13:46:44.14 SSV.dial :exhaust @13:46:44.89 Exhaust.select :open @13:46:45.01 SS.seek 0ml @13:46:53.63 Exhaust.select :close @13:46:53.73 SSV.dial CBV @13:46:53.76
CBV.dial SSV @13:46:54.53 Allowing 130s for 25.0ml of remaining 650.0ml @13:46:54.54 Intake.select :open @13:46:56.71 Intake @16.0psia, vacuum from 10.0 to 13.0psi @13:46:56.73 SS.reconfigure :inhaling 1.76ml/s @13:46:56.77 SS.seek 25ml @13:47:14.28 Stroke completed -- waiting for pressure to equalize ... @13:47:14.29 SS.reconfigure :exhale @13:47:14.34 SS.hold @13:47:32.70 Intake.select :close @13:47:32.86 SSV.dial :exhaust @13:47:33.61 Exhaust.select :open @13:47:33.76 SS.seek 0ml @13:47:42.69 Exhaust.select :close @13:47:42.80 SSV.dial CBV @13:47:42.83
CBV.dial SSV @13:47:43.59 Allowing 130s for 25.0ml of remaining 625.0ml @13:47:43.61 Intake.select :open @13:47:45.77 Intake @16.1psia, vacuum from 10.0 to 13.0psi @13:47:45.79 SS.reconfigure :inhaling 1.76ml/s @13:47:45.84 SS.seek 25ml @13:48:02.76 Stroke completed -- waiting for pressure to equalize ... @13:48:02.77 SS.reconfigure :exhale @13:48:02.82 SS.hold @13:48:21.23 Intake.select :close @13:48:21.39 SSV.dial :exhaust @13:48:22.16 Exhaust.select :open @13:48:22.27 SS.seek 0ml @13:48:30.87 Exhaust.select :close @13:48:30.98 SSV.dial CBV @13:48:31.00
CBV.dial SSV @13:48:31.78 Allowing 130s for 25.0ml of remaining 600.0ml @13:48:31.79 Intake.select :open @13:48:33.96 Intake @16.1psia, vacuum from 10.0 to 13.0psi @13:48:33.98 SS.reconfigure :inhaling 1.76ml/s @13:48:34.03 SS.seek 25ml @13:48:51.26 Stroke completed -- waiting for pressure to equalize ... @13:48:51.27 SS.reconfigure :exhale @13:48:51.32 SS.hold @13:49:11.72 Intake.select :close @13:49:11.87 SSV.dial :exhaust @13:49:12.63 Exhaust.select :open @13:49:12.75 SS.seek 0ml @13:49:21.45 Exhaust.select :close @13:49:21.56 SSV.dial CBV @13:49:21.58
CBV.dial SSV @13:49:22.37 Allowing 130s for 25.0ml of remaining 575.0ml @13:49:22.38 Intake.select :open @13:49:24.55 Intake @16.0psia, vacuum from 10.0 to 13.0psi @13:49:24.57 SS.reconfigure :inhaling 1.76ml/s @13:49:24.61 SS.seek 25ml @13:49:41.90 Stroke completed -- waiting for pressure to equalize ... @13:49:41.91 SS.reconfigure :exhale @13:49:41.96 SS.hold @13:50:00.74 Intake.select :close @13:50:00.90 SSV.dial :exhaust @13:50:01.65 Exhaust.select :open @13:50:01.77 SS.seek 0ml @13:50:10.53 Exhaust.select :close @13:50:10.64 SSV.dial CBV @13:50:10.66
CBV.dial SSV @13:50:11.45 Allowing 130s for 25.0ml of remaining 550.0ml @13:50:11.46 Intake.select :open @13:50:13.63 Intake @16.0psia, vacuum from 10.0 to 13.0psi @13:50:13.64 SS.reconfigure :inhaling 1.76ml/s @13:50:13.69 SS.seek 25ml @13:50:31.12 Stroke completed -- waiting for pressure to equalize ... @13:50:31.13 SS.reconfigure :exhale @13:50:31.18 SS.hold @13:50:51.61 Intake.select :close @13:50:51.76 SSV.dial :exhaust @13:50:52.52 Exhaust.select :open @13:50:52.64 SS.seek 0ml @13:51:01.79 Exhaust.select :close @13:51:01.89 SSV.dial CBV @13:51:01.91
CBV.dial SSV @13:51:02.70 Allowing 130s for 25.0ml of remaining 525.0ml @13:51:02.71 Intake.select :open @13:51:04.88 Intake @16.1psia, vacuum from 10.0 to 13.0psi @13:51:04.90 SS.reconfigure :inhaling 1.76ml/s @13:51:04.95 SS.seek 25ml @13:51:22.34 Stroke completed -- waiting for pressure to equalize ... @13:51:22.35 SS.reconfigure :exhale @13:51:22.40 SS.hold @13:51:42.80 Intake.select :close @13:51:42.95 SSV.dial :exhaust @13:51:43.72 Exhaust.select :open @13:51:43.84 SS.seek 0ml @13:51:52.76 Exhaust.select :close @13:51:52.87 SSV.dial CBV @13:51:52.89
CBV.dial SSV @13:51:54.09 Allowing 130s for 25.0ml of remaining 500.0ml @13:51:54.11 Intake.select :open @13:51:56.28 Intake @16.0psia, vacuum from 10.0 to 13.0psi @13:51:56.29 SS.reconfigure :inhaling 1.76ml/s @13:51:56.34 SS.seek 25ml @13:52:13.29 Stroke completed -- waiting for pressure to equalize ... @13:52:13.30 SS.reconfigure :exhale @13:52:13.35 SS.hold @13:52:33.76 Intake.select :close @13:52:33.91 SSV.dial :exhaust @13:52:34.68 Exhaust.select :open @13:52:34.79 SS.seek 0ml @13:52:43.54 Exhaust.select :close @13:52:43.65 SSV.dial CBV @13:52:43.67
CBV.dial SSV @13:52:44.48 Allowing 130s for 25.0ml of remaining 475.0ml @13:52:44.49 Intake.select :open @13:52:46.66 Intake @16.2psia, vacuum from 10.0 to 13.0psi @13:52:46.67 SS.reconfigure :inhaling 1.76ml/s @13:52:46.72 SS.seek 25ml @13:53:03.76 Vacuum too high -- waiting up to 40 seconds @13:53:03.77 SS.reconfigure :exhale @13:53:03.83 SS.hold @13:53:16.68 Intake @16.4psia, vacuum from 10.0 to 13.0psi @13:53:16.70 SS.reconfigure :inhaling 1.41ml/s @13:53:16.75 SS.seek 25ml @13:53:18.05 Stroke completed -- waiting for pressure to equalize ... @13:53:18.07 SS.reconfigure :exhale @13:53:18.11 SS.hold @13:53:25.59 Intake.select :close @13:53:25.75 SSV.dial :exhaust @13:53:26.51 Exhaust.select :open @13:53:26.63 SS.seek 0ml @13:53:35.32 Exhaust.select :close @13:53:35.43 SSV.dial CBV @13:53:35.45
CBV.dial SSV @13:53:36.26 Allowing 130s for 25.0ml of remaining 450.0ml @13:53:36.27 Intake.select :open @13:53:38.44 Intake @16.2psia, vacuum from 10.0 to 13.0psi @13:53:38.46 SS.reconfigure :inhaling 1.41ml/s @13:53:38.50 SS.seek 25ml @13:53:59.09 Stroke completed -- waiting for pressure to equalize ... @13:53:59.10 SS.reconfigure :exhale @13:53:59.14 SS.hold @13:54:19.63 Intake.select :close @13:54:19.79 SSV.dial :exhaust @13:54:20.56 Exhaust.select :open @13:54:20.68 SS.seek 0ml @13:54:29.57 Exhaust.select :close @13:54:29.68 SSV.dial CBV @13:54:29.71
CBV.dial SSV @13:54:30.46 Allowing 130s for 25.0ml of remaining 425.0ml @13:54:30.47 Intake.select :open @13:54:32.64 Intake @16.1psia, vacuum from 10.0 to 13.0psi @13:54:32.66 SS.reconfigure :inhaling 1.41ml/s @13:54:32.70 SS.seek 25ml @13:54:52.88 Stroke completed -- waiting for pressure to equalize ... @13:54:52.89 SS.reconfigure :exhale @13:54:52.94 SS.hold @13:55:13.44 Intake.select :close @13:55:13.59 SSV.dial :exhaust @13:55:14.78 Exhaust.select :open @13:55:14.90 SS.seek 0ml @13:55:23.84 Exhaust.select :close @13:55:23.94 SSV.dial CBV @13:55:23.96
CBV.dial SSV @13:55:24.76 Allowing 130s for 25.0ml of remaining 400.0ml @13:55:24.77 Intake.select :open @13:55:26.94 Intake @16.0psia, vacuum from 10.0 to 13.0psi @13:55:26.96 SS.reconfigure :inhaling 1.41ml/s @13:55:27.00 SS.seek 25ml @13:55:47.58 Stroke completed -- waiting for pressure to equalize ... @13:55:47.59 SS.reconfigure :exhale @13:55:47.64 SS.hold @13:56:10.12 Intake.select :close @13:56:10.28 SSV.dial :exhaust @13:56:11.04 Exhaust.select :open @13:56:11.16 SS.seek 0ml @13:56:20.30 Exhaust.select :close @13:56:20.41 SSV.dial CBV @13:56:20.44
CBV.dial SSV @13:56:21.21 Allowing 130s for 25.0ml of remaining 375.0ml @13:56:21.22 Intake.select :open @13:56:23.39 Intake @16.2psia, vacuum from 10.0 to 13.0psi @13:56:23.41 SS.reconfigure :inhaling 1.41ml/s @13:56:23.46 SS.seek 25ml @13:56:43.93 Stroke completed -- waiting for pressure to equalize ... @13:56:43.94 SS.reconfigure :exhale @13:56:43.98 SS.hold @13:57:08.39 Intake.select :close @13:57:08.54 SSV.dial :exhaust @13:57:09.32 Exhaust.select :open @13:57:09.43 SS.seek 0ml @13:57:18.29 Exhaust.select :close @13:57:18.40 SSV.dial CBV @13:57:18.44
CBV.dial SSV @13:57:19.19 Allowing 130s for 25.0ml of remaining 350.0ml @13:57:19.20 Intake.select :open @13:57:21.37 Intake @16.1psia, vacuum from 10.0 to 13.0psi @13:57:21.39 SS.reconfigure :inhaling 1.41ml/s @13:57:21.43 SS.seek 25ml @13:57:38.62 Vacuum too high -- waiting up to 40 seconds @13:57:38.63 SS.reconfigure :exhale @13:57:38.69 SS.hold @13:57:50.05 Intake @16.3psia, vacuum from 10.0 to 13.0psi @13:57:50.06 SS.reconfigure :inhaling 1.13ml/s @13:57:50.11 SS.seek 25ml @13:57:54.28 Stroke completed -- waiting for pressure to equalize ... @13:57:54.29 SS.reconfigure :exhale @13:57:54.34 SS.hold @13:58:07.07 Intake.select :close @13:58:07.23 SSV.dial :exhaust @13:58:07.99 Exhaust.select :open @13:58:08.11 SS.seek 0ml @13:58:16.83 Exhaust.select :close @13:58:16.94 SSV.dial CBV @13:58:16.96
CBV.dial SSV @13:58:17.75 Allowing 130s for 25.0ml of remaining 325.0ml @13:58:17.77 Intake.select :open @13:58:19.93 Intake @16.1psia, vacuum from 10.0 to 13.0psi @13:58:19.95 SS.reconfigure :inhaling 1.13ml/s @13:58:20.00 SS.seek 25ml @13:58:41.37 Vacuum too high -- waiting up to 40 seconds @13:58:41.38 SS.reconfigure :exhale @13:58:41.44 SS.hold @13:58:51.75 Intake @16.5psia, vacuum from 10.0 to 13.0psi @13:58:51.76 SS.reconfigure :inhaling 0.91ml/s @13:58:51.81 SS.seek 25ml @13:58:56.19 Stroke completed -- waiting for pressure to equalize ... @13:58:56.20 SS.reconfigure :exhale @13:58:56.25 SS.hold @13:59:08.98 Intake.select :close @13:59:09.13 SSV.dial :exhaust @13:59:09.89 Exhaust.select :open @13:59:10.01 SS.seek 0ml @13:59:18.96 Exhaust.select :close @13:59:19.07 SSV.dial CBV @13:59:19.11
CBV.dial SSV @13:59:19.83 Allowing 130s for 25.0ml of remaining 300.0ml @13:59:19.84 Intake.select :open @13:59:22.01 Intake @16.2psia, vacuum from 10.0 to 13.0psi @13:59:22.02 SS.reconfigure :inhaling 0.91ml/s @13:59:22.07 SS.seek 25ml @13:59:51.59 Stroke completed -- waiting for pressure to equalize ... @13:59:51.60 SS.reconfigure :exhale @13:59:51.65 SS.hold @14:00:12.32 Intake.select :close @14:00:12.48 SSV.dial :exhaust @14:00:13.24 Exhaust.select :open @14:00:13.35 SS.seek 0ml @14:00:22.06 Exhaust.select :close @14:00:22.17 SSV.dial CBV @14:00:22.22
CBV.dial SSV @14:00:22.96 Allowing 130s for 25.0ml of remaining 275.0ml @14:00:22.97 Intake.select :open @14:00:25.13 Intake @16.2psia, vacuum from 10.0 to 13.0psi @14:00:25.14 SS.reconfigure :inhaling 0.91ml/s @14:00:25.19 SS.seek 25ml @14:00:49.05 Vacuum too high -- waiting up to 40 seconds @14:00:49.07 SS.reconfigure :exhale @14:00:49.12 SS.hold @14:00:56.93 Intake @16.4psia, vacuum from 10.0 to 13.0psi @14:00:56.94 SS.reconfigure :inhaling 0.73ml/s @14:00:56.99 SS.seek 25ml @14:01:04.59 Stroke completed -- waiting for pressure to equalize ... @14:01:04.60 SS.reconfigure :exhale @14:01:04.65 SS.hold @14:01:19.46 Intake.select :close @14:01:19.61 SSV.dial :exhaust @14:01:20.38 Exhaust.select :open @14:01:20.49 SS.seek 0ml @14:01:29.24 Exhaust.select :close @14:01:29.34 SSV.dial CBV @14:01:29.36
CBV.dial SSV @14:01:30.16 Allowing 130s for 25.0ml of remaining 250.0ml @14:01:30.17 Intake.select :open @14:01:32.34 Intake @16.2psia, vacuum from 10.0 to 13.0psi @14:01:32.35 SS.reconfigure :inhaling 0.73ml/s @14:01:32.40 SS.seek 25ml @14:02:08.83 Stroke completed -- waiting for pressure to equalize ... @14:02:08.84 SS.reconfigure :exhale @14:02:08.88 SS.hold @14:02:29.28 Intake.select :close @14:02:29.43 SSV.dial :exhaust @14:02:30.21 Exhaust.select :open @14:02:30.75 SS.seek 0ml @14:02:39.70 Exhaust.select :close @14:02:39.82 SSV.dial CBV @14:02:39.86
CBV.dial SSV @14:02:40.59 Allowing 130s for 25.0ml of remaining 225.0ml @14:02:40.60 Intake.select :open @14:02:42.78 Intake @16.2psia, vacuum from 10.0 to 13.0psi @14:02:42.79 SS.reconfigure :inhaling 0.73ml/s @14:02:42.84 SS.seek 25ml @14:03:18.92 Stroke completed -- waiting for pressure to equalize ... @14:03:18.93 SS.reconfigure :exhale @14:03:18.98 SS.hold @14:03:41.32 Intake.select :close @14:03:41.47 SSV.dial :exhaust @14:03:42.23 Exhaust.select :open @14:03:42.35 SS.seek 0ml @14:03:51.15 Exhaust.select :close @14:03:51.68 SSV.dial CBV @14:03:51.73
CBV.dial SSV @14:03:52.47 Allowing 130s for 25.0ml of remaining 200.0ml @14:03:52.48 Intake.select :open @14:03:54.65 Intake @16.2psia, vacuum from 10.0 to 13.0psi @14:03:54.66 SS.reconfigure :inhaling 0.73ml/s @14:03:54.72 SS.seek 25ml @14:04:31.20 Stroke completed -- waiting for pressure to equalize ... @14:04:31.21 SS.reconfigure :exhale @14:04:31.26 SS.hold @14:04:55.76 Intake.select :close @14:04:55.92 SSV.dial :exhaust @14:04:56.68 Exhaust.select :open @14:04:56.80 SS.seek 0ml @14:05:05.48 Exhaust.select :close @14:05:05.59 SSV.dial CBV @14:05:06.04
CBV.dial SSV @14:05:06.79 Allowing 130s for 25.0ml of remaining 175.0ml @14:05:06.81 Intake.select :open @14:05:08.98 Intake @16.2psia, vacuum from 10.0 to 13.0psi @14:05:08.99 SS.reconfigure :inhaling 0.73ml/s @14:05:09.04 SS.seek 25ml @14:05:44.74 Vacuum too high -- waiting up to 40 seconds @14:05:44.75 SS.reconfigure :exhale @14:05:44.81 SS.hold @14:05:57.60 Intake @16.8psia, vacuum from 10.0 to 13.0psi @14:05:57.62 SS.reconfigure :inhaling 0.59ml/s @14:05:57.66 SS.seek 25ml @14:05:58.82 Stroke completed -- waiting for pressure to equalize ... @14:05:58.83 SS.reconfigure :exhale @14:05:58.88 SS.hold @14:06:11.81 Intake.select :close @14:06:11.96 SSV.dial :exhaust @14:06:12.71 Exhaust.select :open @14:06:12.83 SS.seek 0ml @14:06:21.59 Exhaust.select :close @14:06:21.70 SSV.dial CBV @14:06:21.72
CBV.dial SSV @14:06:22.51 Allowing 130s for 25.0ml of remaining 150.0ml @14:06:22.52 Intake.select :open @14:06:24.69 Intake @16.2psia, vacuum from 10.0 to 13.0psi @14:06:24.70 SS.reconfigure :inhaling 0.59ml/s @14:06:24.75 SS.seek 25ml @14:07:09.79 Stroke completed -- waiting for pressure to equalize ... @14:07:09.80 SS.reconfigure :exhale @14:07:09.85 SS.hold @14:07:34.35 Intake.select :close @14:07:34.50 SSV.dial :exhaust @14:07:35.26 Exhaust.select :open @14:07:35.38 SS.seek 0ml @14:07:44.20 Exhaust.select :close @14:07:44.30 SSV.dial CBV @14:07:44.33
CBV.dial SSV @14:07:45.10 Allowing 130s for 25.0ml of remaining 125.0ml @14:07:45.11 Intake.select :open @14:07:47.28 Intake @16.2psia, vacuum from 10.0 to 13.0psi @14:07:47.30 SS.reconfigure :inhaling 0.59ml/s @14:07:47.35 SS.seek 25ml @14:08:32.33 Stroke completed -- waiting for pressure to equalize ... @14:08:32.35 SS.reconfigure :exhale @14:08:32.39 SS.hold @14:08:58.94 Intake.select :close @14:08:59.10 SSV.dial :exhaust @14:08:59.88 Exhaust.select :open @14:08:59.99 SS.seek 0ml @14:09:08.98 Exhaust.select :close @14:09:09.08 SSV.dial CBV @14:09:09.10
CBV.dial SSV @14:09:09.89 Allowing 130s for 25.0ml of remaining 100.0ml @14:09:09.90 Intake.select :open @14:09:12.06 Intake @16.2psia, vacuum from 10.0 to 13.0psi @14:09:12.07 SS.reconfigure :inhaling 0.59ml/s @14:09:12.12 SS.seek 25ml @14:09:50.92 Vacuum too high -- waiting up to 40 seconds @14:09:50.93 SS.reconfigure :exhale @14:09:50.99 SS.hold @14:10:03.84 Intake @17.1psia, vacuum from 10.0 to 13.0psi @14:10:03.86 SS.reconfigure :inhaling 0.47ml/s @14:10:03.91 SS.seek 25ml @14:10:11.08 Stroke completed -- waiting for pressure to equalize ... @14:10:11.09 SS.reconfigure :exhale @14:10:11.14 SS.hold @14:10:29.49 Intake.select :close @14:10:29.65 SSV.dial :exhaust @14:10:30.41 Exhaust.select :open @14:10:30.53 SS.seek 0ml @14:10:39.71 Exhaust.select :close @14:10:39.81 SSV.dial CBV @14:10:39.84
CBV.dial SSV @14:10:41.04 Allowing 130s for 25.0ml of remaining 75.0ml @14:10:41.05 Intake.select :open @14:10:43.22 Intake @16.2psia, vacuum from 10.0 to 13.0psi @14:10:43.23 SS.reconfigure :inhaling 0.47ml/s @14:10:43.28 SS.seek 25ml @14:11:37.88 Stroke completed -- waiting for pressure to equalize ... @14:11:37.89 SS.reconfigure :exhale @14:11:37.94 SS.hold @14:12:04.44 Intake.select :close @14:12:04.60 SSV.dial :exhaust @14:12:05.37 Exhaust.select :open @14:12:05.49 SS.seek 0ml @14:12:14.50 Exhaust.select :close @14:12:14.98 SSV.dial CBV @14:12:15.01
CBV.dial SSV @14:12:15.77 Allowing 130s for 25.0ml of remaining 50.0ml @14:12:15.78 Intake.select :open @14:12:17.96 Intake @16.2psia, vacuum from 10.0 to 13.0psi @14:12:17.97 SS.reconfigure :inhaling 0.47ml/s @14:12:18.02 SS.seek 25ml @14:13:13.22 Stroke completed -- waiting for pressure to equalize ... @14:13:13.23 SS.reconfigure :exhale @14:13:13.28 SS.hold @14:13:39.89 Intake.select :close @14:13:40.05 SSV.dial :exhaust @14:13:40.82 Exhaust.select :open @14:13:40.94 SS.seek 0ml @14:13:49.88 Exhaust.select :close @14:13:49.99 SSV.dial CBV @14:13:50.04
CBV.dial SSV @14:13:50.78 Allowing 130s for 25.0ml of remaining 25.0ml @14:13:50.80 Intake.select :open @14:13:52.96 Intake @16.3psia, vacuum from 10.0 to 13.0psi @14:13:52.98 SS.reconfigure :inhaling 0.47ml/s @14:13:53.02 SS.seek 24.99ml @14:14:48.91 Stroke completed -- waiting for pressure to equalize ... @14:14:48.92 SS.reconfigure :exhale @14:14:48.96 SS.hold @14:15:22.07 Intake.select :close @14:15:22.22 SSV.dial :exhaust @14:15:22.99 Exhaust.select :open @14:15:23.10 SS.seek 0ml @14:15:32.11 Exhaust.select :close @14:15:32.22 Sampled 1000.0ml @14:15:32.23 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @14:15:32.24 Intake.select :close @14:15:32.31 CSV.dial :waste @14:15:32.36 CS.seek 0ml @14:15:32.49 SSV.dial CBV,avoiding: :exhaust @14:15:32.51 CSV.dial :air @14:15:32.53
CBV.dial SSV @14:15:33.31 CS.seek 10ml @14:15:34.43
CTV.dial :air @14:15:35.20 delay 15 seconds @14:15:50.22 SSV.dial :spit,avoiding: :exhaust @14:15:51.54 CTV.dial CSV,avoiding: :intake @14:15:51.57
SS.seek 0ml @14:15:51.69 SSV.dial CBV,avoiding: :exhaust @14:15:57.98 SSV.dial between CBV and plug @14:15:58.47 CBV.dial between SSV and waste2 @14:15:58.85 CSV.dial between air and CTV @14:15:59.18 CS.seek 8.188ml @14:16:04.17 CTV.dial CSV,avoiding: :air @14:16:04.20
CSV.dial CTV @14:16:04.58 CS.reconfigure :pressurize @14:16:04.63 CS.seek 0ml @14:16:18.31 BubblePoint: Intake @24.2psia, Outlet @1.3psia @14:16:18.32 CBV.dial SSV @14:16:18.66 SS.reconfigure :default @14:16:18.71 SS.seek 10ml @14:16:24.25 delay 15 seconds @14:16:39.38 Evacuated: Intake @23.8psia, Outlet @23.6psia @14:16:39.39 SSV.dial :spit,avoiding: :exhaust @14:16:40.43 SS.reconfigure :exhale @14:16:40.47 SS.seek 0ml @14:16:45.45 SS.reconfigure :fast @14:16:45.49 SS.seek 5ml @14:16:48.37 SSV.dial CBV,avoiding: :exhaust @14:16:48.39
CBV.dial SSV @14:16:49.72 CTV.dial :air,avoiding: :intake @14:16:51.03 CSV.dial CTV @14:16:51.06
SS.seek 10ml @14:16:52.61 CSV.dial :air @14:16:53.06 CS.reconfigure :fast @14:16:53.11 CS.seek 0ml @14:16:53.72
SSV.dial :spit,avoiding: :exhaust @14:16:55.04 SS.reconfigure :exhale @14:16:55.09 SS.seek 0ml @14:17:00.26 SS.reconfigure :fast @14:17:00.31 SS.seek 5ml @14:17:00.92 CS.stop @14:17:03.24 SSV.dial CBV,avoiding: :exhaust @14:17:03.26
CBV.dial SSV @14:17:05.60 SSV.dial :spit,avoiding: :exhaust @14:17:06.93 SS.reconfigure :default @14:17:06.97 SS.seek 0ml @14:17:10.12 SS.stop @14:17:10.17 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) Intake.select :close @14:17:10.25 CSV.dial :waste @14:17:10.39 SSV.dial CBV,avoiding: :exhaust @14:17:10.41
CBV.dial SSV @14:17:11.03 CS.seek 0ml @14:17:11.10 CSV.dial :air @14:17:11.75
CTV.dial :air @14:17:11.77 delay 15 seconds @14:17:11.85 CS.seek 10ml @14:17:26.79
SSV.dial :spit,avoiding: :exhaust @14:17:28.10 CTV.dial CSV,avoiding: :intake @14:17:28.13
SS.reconfigure :exhale @14:17:28.21 SS.seek 0ml @14:17:28.64 SSV.dial CBV,avoiding: :exhaust @14:17:36.42 SSV.dial between CBV and plug @14:17:36.91 CBV.dial between SSV and waste2 @14:17:37.30 CSV.dial between air and CTV @14:17:37.61 CS.seek 7.525ml @14:17:44.08 CTV.dial CSV,avoiding: :air @14:17:44.10
CSV.dial CTV @14:17:44.47 CS.reconfigure :pressurize @14:17:44.52 CS.seek 0ml @14:18:00.49 BubblePoint: Intake @24.1psia, Outlet @3.3psia @14:18:00.50 CBV.dial SSV @14:18:00.85 SS.reconfigure :default @14:18:00.89 SS.seek 10ml @14:18:06.47 delay 15 seconds @14:18:21.60 Evacuated: Intake @23.9psia, Outlet @23.8psia @14:18:21.62 SSV.dial :spit,avoiding: :exhaust @14:18:22.66 SS.reconfigure :exhale @14:18:22.71 SS.seek 0ml @14:18:28.13 SS.reconfigure :fast @14:18:28.18 SS.seek 5ml @14:18:31.21 SSV.dial CBV,avoiding: :exhaust @14:18:31.23
CBV.dial SSV @14:18:32.56 CTV.dial :air,avoiding: :intake @14:18:33.89 CSV.dial CTV @14:18:33.92
SS.seek 10ml @14:18:35.13 CSV.dial :air @14:18:35.58 CS.reconfigure :fast @14:18:35.63 CS.seek 0ml @14:18:36.67
SSV.dial :spit,avoiding: :exhaust @14:18:37.99 SS.reconfigure :exhale @14:18:38.04 SS.seek 0ml @14:18:39.82 CS.stop @14:18:43.05
SS.reconfigure :fast @14:18:43.10 SS.seek 5ml @14:18:45.88 SSV.dial CBV,avoiding: :exhaust @14:18:45.91
CBV.dial SSV @14:18:48.22 SSV.dial :spit,avoiding: :exhaust @14:18:49.55 SS.reconfigure :default @14:18:49.60 SS.seek 0ml @14:18:52.82 SS.stop @14:18:52.86 Lysing: Adding 2.0ml AqMeOH @14:18:52.88 CBV.dial between CSV and waste1,avoiding: :DBV @14:18:54.40 CTV.dial CSV @14:18:55.73 CSV.dial CSR @14:18:56.65 Pulling 0.999ml of CSR.AqMeOH CSR.select :AqMeOH @14:18:56.77 CS.reconfigure :default @14:18:56.81 CS.seek 1ml @14:19:00.29 delay 5 seconds @14:19:05.31 CSR.select :air @14:19:05.48 CS.seek 6.001ml @14:19:19.07 delay 2 seconds @14:19:21.09 CSV.dial :waste2 @14:19:21.67 CS.reconfigure :fast @14:19:21.72 CS.seek 0ml @14:19:36.20 CSV.dial CSR @14:19:36.63 Pulling 2ml of CSR.AqMeOH @14:19:36.64 CSR.select :AqMeOH @14:19:36.76 CS.reconfigure :default @14:19:36.80 CS.seek 2ml @14:19:42.73 delay 5 seconds @14:19:47.75 CSR.select :air @14:19:47.91 CS.seek 5ml @14:19:56.42 delay 2 seconds @14:19:58.43 CSV.dial CTV @14:19:58.87 CS.seek 1ml @14:20:10.48 Before lysing Collection Puck Heater at 12.7C/29.9psia @14:20:10.50 Heating Puck.from 6, :sh1 in Collection Clamp to 70C for 10:00 @14:20:10.52 CH.seek 70C,8:00 @14:22:26.78 CH.hold 10:00,70C @14:22:26.97 Begun lysing Collection Puck Heater at 68C/37.0psia @14:32:26.99 Ended lysing Collection Puck Heater at 70C/35.8psia @14:32:27.01 CS.seek 0ml @14:32:30.82 CTV.dial between spare and air,avoiding: :intake @14:32:31.84 CSV.dial :air @14:32:32.30 CS.seek 5ml @14:32:46.12 CSV.dial CTV @14:32:46.58 delay 2 seconds @14:32:48.59 CSV.dial :air @14:32:49.05 delay 2 seconds @14:32:51.06 CSV.dial CTV @14:32:51.52 delay 2 seconds @14:32:53.54 CSV.dial :air @14:32:53.99 delay 2 seconds @14:32:56.01 CS.seek 0ml @14:33:09.67 Puck @78.2psia after final lysis pressurization @14:33:09.69 Recovering Lysate into Collection syringe @14:33:09.70 CSV.dial CBV @14:33:10.73 CBV.dial CSV @14:33:11.11 CS.seek 5ml @14:33:24.68 delay 5 seconds @14:33:29.70 CTV.dial :air @14:33:30.25 CS.seek 6.999ml @14:33:36.12 delay 5 seconds @14:33:41.14 CSV.dial :air2 @14:33:41.58 CS.seek 10ml @14:33:50.06 CSV.dial between air and CTV,avoiding: CBV @14:33:51.34 Lysing Complete @14:33:51.41 Storing sh1 puck in Collection Clamp to tube 5 @14:33:51.59 SC.reconfigure :tight @14:33:51.63 SC.seek 5 @14:33:55.21 SC.configure :limp @14:33:55.87 Elbow.reconfigure :tight @14:33:55.92 Elbow.seek 5 @14:33:57.71 Forearm.reconfigure :tight @14:33:57.76 Forearm.seek 5 + 450 counts @14:33:58.82 SE.reconfigure :pick @14:33:58.88 SE.seek :beamBreak @14:34:01.26 SE.configure :default @14:34:01.42 Elbow.reconfigure :limp @14:34:01.47 Forearm.reconfigure :loose @14:34:01.52 Forearm.seek 5 @14:34:01.79 Elbow.hold @14:34:01.86 Elbow.reconfigure :tight @14:34:01.96 SE.seek 11.16 Pucks below Top Plate @14:34:02.43 Forearm.force -20 @14:34:02.48 Forearm.seek 5 + 276 counts @14:34:02.77 Forearm.reconfigure :tight @14:34:02.82 Forearm.seek 5 + 450 counts @14:34:03.06 SE.seek 12.16 Pucks below Top Plate @14:34:03.67 Forearm.reconfigure :loose @14:34:03.72 Forearm.seek :retract @14:34:04.64 Elbow.seek CC @14:34:06.35 Elbow.reconfigure :loose @14:34:06.45 Forearm.seek CC @14:34:07.48 Hand.close @14:34:08.09 CC.seek :open @14:34:12.82 Forearm.seek :retract @14:34:13.79 Elbow.reconfigure :tight @14:34:13.84 Elbow.seek 5 @14:34:15.98 Elbow.reconfigure :limp @14:34:16.10 Forearm.seek 5 @14:34:17.13 Elbow.hold @14:34:17.21 Elbow.reconfigure :tight @14:34:17.25 Hand.open @14:34:17.97 Forearm.force -20 @14:34:18.01 Forearm.seek 5 + 315 counts @14:34:18.30 Forearm.reconfigure :tight @14:34:18.35 Forearm.seek 5 + 450 counts @14:34:18.61 SE.seek :down @14:34:19.74
Forearm.reconfigure :loose @14:34:19.79 Forearm.seek :retract @14:34:20.50 SE.stop @14:34:20.72
Tranferring DA @14:34:20.73 Beginning samplePassC @14:34:20.74 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:34:20.93 Elbow.seek FlushPuck::Garage @14:34:22.77 Elbow.reconfigure :loose @14:34:22.88 Forearm.seek :garage @14:34:23.92 Hand.close @14:34:24.71 Forearm.seek :retract @14:34:25.72 Elbow.reconfigure :tight @14:34:25.76 Elbow.seek CC @14:34:28.68 Elbow.reconfigure :loose @14:34:28.78 Forearm.reconfigure :tight @14:34:28.83 Forearm.seek CC @14:34:30.05 CC.seek :closed @14:34:34.57 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.43 @14:34:34.63 Hand.open @14:34:35.28 Forearm.reconfigure :loose @14:34:35.32 Forearm.seek :retract @14:34:36.31 PSV.dial PSR @14:34:37.70 Pulling 1.5ml of PSR.flush @14:34:37.71 PSR.select :flush @14:34:37.83 PS.seek 1.5ml @14:34:53.94 delay 5 seconds @14:34:58.96 PSV.dial :waste1 @14:34:59.46 PS.seek 0ml @14:35:15.62 PSV.dial PSR @14:35:16.10 PSR.select :air @14:35:16.22 PS.seek 2.5ml @14:35:42.66 PRV.dial CBV,avoiding: :ab2 @14:35:42.68
CBV.dial PRV @14:35:44.42 PSV.dial :mixloop @14:35:45.47 CTV.dial :air,avoiding: :intake @14:35:45.65 PTV.dial :PRVmixing @14:35:46.99 PS.seek 0ml @14:36:13.40 PSV.dial :air @14:36:13.88 PS.seek 2.5ml @14:36:40.28 PSV.dial :mixloop @14:36:40.75 PS.seek 0ml @14:37:07.06 PSV.dial :air @14:37:07.54 PS.seek 2.5ml @14:37:33.96 PSV.dial :mixloop @14:37:34.44 PS.seek 0ml @14:38:00.81 Garaging FlushPuck in Collection Clamp @14:38:00.88 Elbow.reconfigure :tight @14:38:00.93 Elbow.seek CC @14:38:01.19 Elbow.reconfigure :loose @14:38:01.29 Forearm.seek CC @14:38:02.30 Hand.close @14:38:03.03 CC.seek :open @14:38:07.65 Forearm.seek :retract @14:38:08.70 Elbow.reconfigure :tight @14:38:08.75 Elbow.seek :garage @14:38:11.64 Elbow.reconfigure :loose @14:38:11.74 Forearm.reconfigure :tight @14:38:11.79 Forearm.seek :garage @14:38:12.87 Hand.open @14:38:13.52 Forearm.reconfigure :loose @14:38:13.57 Forearm.seek :retract @14:38:14.54 PSV.dial :mixloop @14:38:14.61 PTV.dial :PRVmixing @14:38:14.69 PRV.dial CBV @14:38:14.71
CBV.dial PRV @14:38:14.85 CTV.dial :air,avoiding: :intake @14:38:14.87 Loading lyfil puck from tube 6 into Collection Clamp @14:38:15.11 SC.reconfigure :tight @14:38:15.16 SC.seek 6 @14:38:18.73 SC.configure :limp @14:38:18.95 Elbow.reconfigure :tight @14:38:19.00 Elbow.seek 6 @14:38:20.91 Forearm.reconfigure :tight @14:38:20.96 Forearm.seek 6 + 450 counts @14:38:22.05 SE.reconfigure :pick @14:38:22.11 SE.seek :beamBreak @14:38:24.02 SE.configure :default @14:38:24.19 Elbow.seek 6 @14:38:24.37 Forearm.seek 6 + 300 counts @14:38:24.61 Forearm.reconfigure :loose @14:38:25.10 Hand.close @14:38:25.73 SE.seek :down @14:38:25.93
Forearm.seek :retract @14:38:26.88 Elbow.seek CC @14:38:27.24 SE.stop @14:38:28.49
Elbow.reconfigure :loose @14:38:28.59 Forearm.reconfigure :tight @14:38:28.64 Forearm.seek CC @14:38:29.92 CC.seek :closed @14:38:34.44 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.16 @14:38:34.45 Hand.open @14:38:35.14 Forearm.reconfigure :loose @14:38:35.19 Forearm.seek :retract @14:38:36.21 CTV.dial CSV,avoiding: :intake @14:38:37.54 CSV.dial CTV @14:38:37.84 PS.seek 2.5ml @14:39:04.28 CS.seek 4.5ml @14:39:19.21 delay 8 seconds @14:39:27.23 PSV.dial :air @14:39:27.70 delay 2 seconds @14:39:29.72 PSV.dial :waste2 @14:39:30.45 PS.seek 0ml @14:39:56.84 CBV.dial between PRV and waste1 @14:39:57.16 CSV.dial :air @14:39:57.62 CS.seek 10ml @14:40:12.49 CSV.dial CTV @14:40:12.95 CS.seek 0ml @14:40:39.55 CBV.dial :waste1 @14:40:40.32 delay 5 seconds @14:40:45.34 CTV.dial :air,avoiding: :intake @14:40:46.74 Storing lyfil puck in Collection Clamp to tube 5 @14:40:46.92 SC.reconfigure :tight @14:40:46.97 SC.seek 5 @14:40:50.51 SC.configure :limp @14:40:50.73 Elbow.reconfigure :tight @14:40:50.78 Elbow.seek 5 @14:40:52.55 Forearm.reconfigure :tight @14:40:52.59 Forearm.seek 5 + 450 counts @14:40:53.63 SE.reconfigure :pick @14:40:53.68 SE.seek :beamBreak @14:40:56.02 SE.configure :default @14:40:56.19 Elbow.reconfigure :limp @14:40:56.24 Forearm.reconfigure :loose @14:40:56.28 Forearm.seek 5 @14:40:56.52 Elbow.hold @14:40:56.58 Elbow.reconfigure :tight @14:40:56.69 SE.seek 12.16 Pucks below Top Plate @14:40:57.21 Forearm.force -20 @14:40:57.25 Forearm.seek 5 + 319 counts @14:40:57.55 Forearm.reconfigure :tight @14:40:57.59 Forearm.seek 5 + 450 counts @14:40:57.79 SE.seek 13.16 Pucks below Top Plate @14:40:58.39 Forearm.reconfigure :loose @14:40:58.44 Forearm.seek :retract @14:40:59.38 Elbow.seek CC @14:41:01.04 Elbow.reconfigure :loose @14:41:01.58 Forearm.seek CC @14:41:02.55 Hand.close @14:41:03.21 CC.seek :open @14:41:07.95 Forearm.seek :retract @14:41:08.98 Elbow.reconfigure :tight @14:41:09.02 Elbow.seek 5 @14:41:10.78 Elbow.reconfigure :limp @14:41:10.83 Forearm.seek 5 @14:41:11.88 Elbow.hold @14:41:11.95 Elbow.reconfigure :tight @14:41:12.00 Hand.open @14:41:12.70 Forearm.force -20 @14:41:12.76 Forearm.seek 5 + 314 counts @14:41:13.04 Forearm.reconfigure :tight @14:41:13.09 Forearm.seek 5 + 450 counts @14:41:13.35 SE.seek :down @14:41:14.49
Forearm.reconfigure :loose @14:41:14.54 Forearm.seek :retract @14:41:15.17 SE.stop @14:41:15.49
PSV.dial :air @14:41:16.25 PS.seek 2.5ml @14:41:42.59 delay 1 second @14:41:43.60 PSV.dial :mixloop @14:41:44.06 delay 1 second @14:41:45.07 PSV.dial :air @14:41:45.54 delay 1 second @14:41:46.56 PSV.dial :mixloop @14:41:47.03 delay 1 second @14:41:48.04 PSV.dial :air @14:41:48.51 delay 1 second @14:41:49.53 PS.seek 0ml @14:42:15.87 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @14:42:16.49 Elbow.seek FlushPuck::Garage @14:42:18.31 Elbow.reconfigure :loose @14:42:18.42 Forearm.seek :garage @14:42:19.37 Hand.close @14:42:20.08 Forearm.seek :retract @14:42:21.09 Elbow.reconfigure :tight @14:42:21.14 Elbow.seek PC @14:42:22.42 Elbow.reconfigure :loose @14:42:22.52 Forearm.reconfigure :tight @14:42:22.57 Forearm.seek PC @14:42:23.86 PC.seek :closed @14:42:28.51 Processing Clamp onSpring/offSpring for 16/26 samples, on/off torque 1.66 @14:42:28.58 Hand.open @14:42:29.27 Forearm.reconfigure :loose @14:42:29.32 Forearm.seek :retract @14:42:30.31 PRV.dial :air @14:42:30.77 PSV.dial :mixloop @14:42:31.31 PS.seek 0.3ml @14:42:35.21 PSV.dial PSR @14:42:36.28 Pulling 0.4ml of PSR.air @14:42:36.29 PSR.select :air @14:42:36.31 PS.seek 0.7ml @14:42:41.40 delay 3 seconds @14:42:44.42 PSR.select :air @14:42:44.45 PS.seek 2.5ml @14:43:03.68 delay 3 seconds @14:43:06.70 PSV.dial :mixloop @14:43:07.76 PTV.dial :mixing @14:43:08.96 PBV.dial :waste2 @14:43:09.94 PS.seek 0ml @14:43:36.46 Garaging FlushPuck in Processing Clamp @14:43:36.54 Elbow.reconfigure :tight @14:43:36.58 Elbow.seek PC @14:43:36.71 Elbow.reconfigure :loose @14:43:36.81 Forearm.seek PC @14:43:37.78 Hand.close @14:43:38.46 PC.seek :open @14:43:43.18 Forearm.seek :retract @14:43:44.19 Elbow.reconfigure :tight @14:43:44.24 Elbow.seek :garage @14:43:45.61 Elbow.reconfigure :loose @14:43:45.71 Forearm.reconfigure :tight @14:43:45.76 Forearm.seek :garage @14:43:46.81 Hand.open @14:43:47.52 Forearm.reconfigure :loose @14:43:47.56 Forearm.seek :retract @14:43:48.60 Loading da puck from tube 6 into Processing Clamp @14:43:48.84 SC.reconfigure :tight @14:43:48.89 SC.seek 6 @14:43:52.51 SC.configure :limp @14:43:52.73 Elbow.reconfigure :tight @14:43:52.78 Elbow.seek 6 @14:43:54.67 Forearm.reconfigure :tight @14:43:54.72 Forearm.seek 6 + 450 counts @14:43:55.76 SE.reconfigure :pick @14:43:55.82 SE.seek :beamBreak @14:43:57.78 SE.configure :default @14:43:57.94 Elbow.seek 6 @14:43:58.57 Forearm.seek 6 + 300 counts @14:43:58.81 Forearm.reconfigure :loose @14:43:58.86 Hand.close @14:43:59.47 SE.seek :down @14:43:59.67
Forearm.seek :retract @14:44:00.71 Elbow.seek PC @14:44:01.09 SE.stop @14:44:03.26
Elbow.reconfigure :loose @14:44:03.36 Forearm.reconfigure :tight @14:44:03.41 Forearm.seek PC @14:44:04.69 PC.seek :closed @14:44:09.35 Processing Clamp onSpring/offSpring for 16/26 samples, on/off torque 1.66 @14:44:09.36 Hand.open @14:44:10.01 Forearm.reconfigure :loose @14:44:10.06 Forearm.seek :retract @14:44:11.04 Heating Puck.from 6, :da in Processing Clamp to 28C for 2:00:00 @14:44:11.06 PH.seek 28C,5:00 @14:44:53.78 PH.hold 2:00:00,28C @14:44:53.84 delay 1:30 @14:46:23.87 PSR.select :air @14:46:23.89 PSV.dial PSR @14:46:24.96 Pulling 0.5ml of PSR.wash @14:46:24.97 PSR.select :wash @14:46:25.09 PS.seek 0.5ml @14:46:30.99 delay 5 seconds @14:46:36.01 PSR.select :air @14:46:36.13 PS.seek 2ml @14:46:52.33 PTV.dial :puck @14:46:53.08 PSV.dial PTV @14:46:53.97 PBV.dial :waste1 @14:46:55.36 PS.seek 1.5ml @14:47:01.30 delay 3 seconds @14:47:04.32 PBV.dial between PSV and waste1 @14:47:04.68 PS.seek 0ml @14:47:20.86 delay 5:00 @14:52:20.88 recoverPSRreagent08 @14:52:20.89 PTV.dial :puck @14:52:20.96 PBV.dial :waste1 @14:52:21.34 delay 30 seconds @14:52:51.37 PBV.dial between PSV and waste1 @14:52:51.75 PSR.select :air @14:52:51.77 PSV.dial PSR @14:52:52.52 PS.reconfigure :fast @14:52:52.57 PS.seek 2.5ml @14:53:16.48 PSV.dial PTV @14:53:17.25 delay 5 seconds @14:53:22.27 PSV.dial PSR @14:53:23.01 delay 5 seconds @14:53:28.03 PSV.dial PTV @14:53:28.86 delay 5 seconds @14:53:33.88 PSV.dial :air @14:53:34.77 PS.seek 0ml @14:53:57.95 PTV.dial :PRVmixing,via: PRV @14:53:59.29 PSV.dial :mixloop @14:53:59.74 PRV.dial :ab1 @14:54:00.52 PS.reconfigure :slow @14:54:00.57 PS.seek 0.15ml @14:54:04.23 delay 2 seconds @14:54:06.25 CBV.dial PRV @14:54:06.27
PRV.dial CBV @14:54:06.86 PS.seek 0.35ml @14:54:11.51 delay 2 seconds @14:54:13.53 CBV.dial between PRV and PSV @14:54:13.84 PRV.dial :air @14:54:14.30 PS.seek 0.9ml @14:54:26.07 PRV.dial :waste2 @14:54:26.83 PS.seek 0.5ml @14:54:35.67 addPMLReagent08 @14:54:35.68 PSV.dial :air @14:54:36.14 PS.reconfigure :default @14:54:36.19 PS.seek 2ml @14:54:52.29 PSV.dial :PML @14:54:52.76 PTV.dial :mixing @14:54:53.51 PBV.dial :waste1 @14:54:53.94 PS.seek 1.85ml @14:54:56.32 PBV.dial between PSV and waste1 @14:54:56.70 PS.seek 0ml @14:55:16.45 delay 2 seconds @14:55:18.47 Beginning samplePassC @14:55:18.48 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:55:18.66 Elbow.reconfigure :tight @14:55:18.71 Elbow.seek FlushPuck::Garage @14:55:20.07 Elbow.reconfigure :loose @14:55:20.17 Forearm.seek :garage @14:55:21.12 Hand.close @14:55:21.82 Forearm.seek :retract @14:55:22.81 Elbow.reconfigure :tight @14:55:22.86 Elbow.seek CC @14:55:25.71 Elbow.reconfigure :loose @14:55:26.24 Forearm.reconfigure :tight @14:55:26.29 Forearm.seek CC @14:55:27.66 CC.seek :closed @14:55:32.18 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.52 @14:55:32.24 Hand.open @14:55:32.90 Forearm.reconfigure :loose @14:55:32.94 Forearm.seek :retract @14:55:33.93 PSV.dial PSR @14:55:34.99 Pulling 1.5ml of PSR.flush @14:55:35.00 PSR.select :flush @14:55:35.13 PS.seek 1.5ml @14:55:51.29 delay 5 seconds @14:55:56.31 PSV.dial :waste1 @14:55:56.78 PS.seek 0ml @14:56:13.01 PSV.dial PSR @14:56:13.48 PSR.select :air @14:56:13.60 PS.seek 2.5ml @14:56:39.92 PRV.dial CBV,avoiding: :ab2 @14:56:39.94
CBV.dial PRV @14:56:41.59 PSV.dial :mixloop @14:56:42.64 CTV.dial :air,avoiding: :intake @14:56:42.71 PTV.dial :PRVmixing @14:56:43.48 PS.seek 0ml @14:57:09.76 PSV.dial :air @14:57:10.23 PS.seek 2.5ml @14:57:36.57 PSV.dial :mixloop @14:57:37.04 PS.seek 0ml @14:58:03.41 PSV.dial :air @14:58:03.89 PS.seek 2.5ml @14:58:30.19 PSV.dial :mixloop @14:58:30.74 PS.seek 0ml @14:58:57.13 Garaging FlushPuck in Collection Clamp @14:58:57.21 Elbow.reconfigure :tight @14:58:57.25 Elbow.seek CC @14:58:57.55 Elbow.reconfigure :loose @14:58:57.65 Forearm.seek CC @14:58:58.68 Hand.close @14:58:59.32 CC.seek :open @14:59:03.97 Forearm.seek :retract @14:59:04.95 Elbow.reconfigure :tight @14:59:05.00 Elbow.seek :garage @14:59:07.88 Elbow.reconfigure :loose @14:59:07.98 Forearm.reconfigure :tight @14:59:08.03 Forearm.seek :garage @14:59:09.04 Hand.open @14:59:09.75 Forearm.reconfigure :loose @14:59:09.80 Forearm.seek :retract @14:59:10.77 delayUntil "15:03:18.45" @14:59:10.80 Whole Cell Archive sampling 1000ml @14:59:10.82 Loading wcr puck from tube 6 into Collection Clamp @14:59:11.07 SC.reconfigure :tight @14:59:11.12 SC.seek 6 @14:59:11.18 SC.configure :limp @14:59:11.40 Elbow.reconfigure :tight @14:59:11.45 Elbow.seek 6 @14:59:13.42 Forearm.reconfigure :tight @14:59:13.47 Forearm.seek 6 + 450 counts @14:59:14.49 SE.reconfigure :pick @14:59:14.55 SE.seek :beamBreak @14:59:16.57 SE.configure :default @14:59:17.18 Elbow.seek 6 @14:59:17.38 Forearm.seek 6 + 300 counts @14:59:17.58 Forearm.reconfigure :loose @14:59:17.63 Hand.close @14:59:18.21 SE.seek :down @14:59:18.42 Forearm.seek :retract @14:59:19.35 Elbow.seek CC @14:59:19.84 SE.stop @14:59:20.89 Elbow.reconfigure :loose @14:59:21.00 Forearm.reconfigure :tight @14:59:21.06 Forearm.seek CC @14:59:22.38 CC.seek :closed @14:59:26.92 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.35 @14:59:26.93 Hand.open @14:59:27.63 Forearm.reconfigure :loose @14:59:27.68 Forearm.seek :retract @14:59:28.65 collectSample 1000ml w/bubble point of 35psi @14:59:28.66 Priming 7ml @14:59:28.67 Intake.select :close @14:59:28.68 Exhaust.select :close @14:59:28.70 SS.hold @14:59:28.91 Calibrating pressure sensors @14:59:28.92 CTV.dial :air @14:59:29.01 SSV.dial :spit,avoiding: :exhaust @14:59:29.10 SS.reconfigure :fast @14:59:29.60 SS.seek 5ml @14:59:32.56 SSV.dial CBV @14:59:32.59 CBV.dial SSV @14:59:33.94 delay 3.8 seconds @14:59:37.88 Sensor limits: Intake max 101.6psia; Outlet max 93.5psia @14:59:37.89 SSV.dial :spit @14:59:39.25 SS.seek 0ml @14:59:41.87 SSV.dial CBV @14:59:43.20 CTV.dial :intake @14:59:43.98 SSV.dial CBV @14:59:44.00 CBV.dial SSV @14:59:44.10 Allowing 40s for 7.0ml of remaining 7.0ml @14:59:44.11 Intake.select :open @14:59:46.29 Intake @14.3psia, vacuum from 8.8 to 11.8psi @14:59:46.30 SS.reconfigure :inhaling 2.20ml/s @14:59:46.35 SS.seek 7ml @14:59:53.21 Stroke completed -- waiting for pressure to equalize ... @14:59:53.23 SS.reconfigure :exhale @14:59:53.27 SS.hold @15:00:13.71 Intake.select :close @15:00:13.87 SSV.dial :exhaust @15:00:14.64 Exhaust.select :open @15:00:14.76 SS.seek 0ml @15:00:19.04 Exhaust.select :close @15:00:19.15 Sampling 1000.0ml @15:00:19.17 Intake.select :close @15:00:19.18 Exhaust.select :close @15:00:19.19 SS.reconfigure :default @15:00:19.24 SS.hold @15:00:19.45 CTV.dial :intake @15:00:19.54 SSV.dial CBV @15:00:19.56 CBV.dial SSV @15:00:20.34 Allowing 130s for 25.0ml of remaining 1000.0ml @15:00:20.36 Intake.select :open @15:00:22.52 Intake @15.2psia, vacuum from 9.7 to 12.7psi @15:00:22.54 SS.reconfigure :inhaling 2.20ml/s @15:00:22.59 SS.seek 25ml @15:00:30.11 Vacuum too high -- waiting up to 40 seconds @15:00:30.13 SS.reconfigure :exhale @15:00:30.19 SS.hold @15:00:38.08 Intake @16.4psia, vacuum from 10.0 to 13.0psi @15:00:38.10 SS.reconfigure :inhaling 1.76ml/s @15:00:38.14 SS.seek 25ml @15:00:42.52 Vacuum too high -- waiting up to 40 seconds @15:00:42.54 SS.reconfigure :exhale @15:00:42.59 SS.hold @15:00:47.72 Intake @17.0psia, vacuum from 10.0 to 13.0psi @15:00:47.74 SS.reconfigure :inhaling 1.41ml/s @15:00:47.79 SS.seek 25ml @15:00:52.36 Vacuum too high -- waiting up to 40 seconds @15:00:52.38 SS.reconfigure :exhale @15:00:52.43 SS.hold @15:00:56.94 Intake @17.2psia, vacuum from 10.0 to 13.0psi @15:00:56.96 SS.reconfigure :inhaling 1.13ml/s @15:00:57.01 SS.seek 25ml @15:00:59.67 Stroke completed -- waiting for pressure to equalize ... @15:00:59.68 SS.reconfigure :exhale @15:00:59.73 SS.hold @15:01:32.92 Intake.select :close @15:01:33.08 SSV.dial :exhaust @15:01:33.86 Exhaust.select :open @15:01:33.98 SS.seek 0ml @15:01:42.88 Exhaust.select :close @15:01:42.99 SSV.dial CBV @15:01:43.04 CBV.dial SSV @15:01:43.78 Allowing 130s for 25.0ml of remaining 975.0ml @15:01:43.79 Intake.select :open @15:01:45.96 Intake @15.5psia, vacuum from 10.0 to 13.0psi @15:01:45.98 SS.reconfigure :inhaling 1.13ml/s @15:01:46.03 SS.seek 25ml @15:02:05.45 Vacuum too high -- waiting up to 40 seconds @15:02:05.47 SS.reconfigure :exhale @15:02:05.53 SS.hold @15:02:13.15 Intake @15.9psia, vacuum from 10.0 to 13.0psi @15:02:13.17 SS.reconfigure :inhaling 0.91ml/s @15:02:13.22 SS.seek 25ml @15:02:20.55 Stroke completed -- waiting for pressure to equalize ... @15:02:20.57 SS.reconfigure :exhale @15:02:20.62 SS.hold @15:02:55.40 Intake.select :close @15:02:55.56 SSV.dial :exhaust @15:02:56.32 Exhaust.select :open @15:02:56.44 SS.seek 0ml @15:03:05.26 Exhaust.select :close @15:03:05.37 SSV.dial CBV @15:03:05.41 CBV.dial SSV @15:03:06.17 Allowing 130s for 25.0ml of remaining 950.0ml @15:03:06.18 Intake.select :open @15:03:08.35 Intake @15.6psia, vacuum from 10.0 to 13.0psi @15:03:08.37 SS.reconfigure :inhaling 0.91ml/s @15:03:08.42 SS.seek 25ml @15:03:18.47 recoverPRVreagent08 @15:03:18.48 PTV.dial :mixing @15:03:19.23 PBV.dial :waste1 @15:03:19.63 delay 30 seconds @15:03:38.44 Stroke completed -- waiting for pressure to equalize ... @15:03:38.46 SS.reconfigure :exhale @15:03:38.50 SS.hold @15:03:49.71 PBV.dial between PSV and waste1 @15:03:50.09 PSV.dial :air @15:03:50.69 PS.reconfigure :fast @15:03:50.74 PS.seek 2.5ml @15:04:13.33 Intake.select :close @15:04:13.49 SSV.dial :exhaust @15:04:14.26 Exhaust.select :open @15:04:14.38 SS.seek 0ml @15:04:14.90 PSV.dial :mixloop @15:04:15.36 delay 2 seconds @15:04:17.38 PSV.dial :air @15:04:17.97 delay 2 seconds @15:04:19.99 PSV.dial :mixloop @15:04:20.51 delay 2 seconds @15:04:22.53 PSV.dial :air @15:04:22.99 PS.seek 0ml @15:04:23.37 Exhaust.select :close @15:04:23.48 SSV.dial CBV @15:04:23.53 CBV.dial SSV @15:04:24.27 Allowing 130s for 25.0ml of remaining 925.0ml @15:04:24.29 Intake.select :open @15:04:26.46 Intake @15.7psia, vacuum from 10.0 to 13.0psi @15:04:26.48 SS.reconfigure :inhaling 0.91ml/s @15:04:26.53 SS.seek 25ml @15:04:46.66 washCycleDA #1 of 2 @15:04:46.67 PSR.select :air @15:04:46.68 PSV.dial PSR @15:04:48.03 Pulling 0.5ml of PSR.wash @15:04:48.04 PSR.select :wash @15:04:48.16 PS.reconfigure :default @15:04:48.21 PS.seek 0.5ml @15:04:54.44 delay 5 seconds @15:04:56.05 Stroke completed -- waiting for pressure to equalize ... @15:04:56.07 SS.reconfigure :exhale @15:04:56.11 SS.hold @15:04:59.45 PSR.select :air @15:04:59.66 PS.seek 2ml @15:05:15.90 PTV.dial :puck @15:05:16.67 PSV.dial PTV @15:05:17.54 PBV.dial :waste1 @15:05:17.98 PS.seek 1.5ml @15:05:23.93 delay 3 seconds @15:05:26.95 PBV.dial between PSV and waste1 @15:05:27.33 PS.seek 0ml @15:05:30.89 Intake.select :close @15:05:31.06 SSV.dial :exhaust @15:05:31.82 Exhaust.select :open @15:05:31.94 SS.seek 0ml @15:05:40.75 Exhaust.select :close @15:05:40.86 SSV.dial CBV @15:05:40.90 CBV.dial SSV @15:05:41.66 Allowing 130s for 25.0ml of remaining 900.0ml @15:05:41.68 Intake.select :open @15:05:43.56 delay 2:00 @15:05:43.85 Intake @15.6psia, vacuum from 10.0 to 13.0psi @15:05:43.87 SS.reconfigure :inhaling 0.91ml/s @15:05:43.91 SS.seek 25ml @15:06:13.82 Stroke completed -- waiting for pressure to equalize ... @15:06:13.83 SS.reconfigure :exhale @15:06:13.88 SS.hold @15:06:42.76 Intake.select :close @15:06:42.92 SSV.dial :exhaust @15:06:43.68 Exhaust.select :open @15:06:43.79 SS.seek 0ml @15:06:52.82 Exhaust.select :close @15:06:53.39 SSV.dial CBV @15:06:53.42 CBV.dial SSV @15:06:54.18 Allowing 130s for 25.0ml of remaining 875.0ml @15:06:54.19 Intake.select :open @15:06:56.36 Intake @15.8psia, vacuum from 10.0 to 13.0psi @15:06:56.38 SS.reconfigure :inhaling 0.91ml/s @15:06:56.43 SS.seek 25ml @15:07:25.94 Stroke completed -- waiting for pressure to equalize ... @15:07:25.95 SS.reconfigure :exhale @15:07:26.00 SS.hold @15:07:43.58 recoverPSRreagent08 @15:07:43.59 PTV.dial :puck @15:07:43.78 PBV.dial :waste1 @15:07:44.15 delay 30 seconds @15:07:58.73 Intake.select :close @15:07:58.90 SSV.dial :exhaust @15:07:59.67 Exhaust.select :open @15:07:59.79 SS.seek 0ml @15:08:08.75 Exhaust.select :close @15:08:08.86 SSV.dial CBV @15:08:08.88 CBV.dial SSV @15:08:09.66 Allowing 130s for 25.0ml of remaining 850.0ml @15:08:09.68 Intake.select :open @15:08:11.84 Intake @15.6psia, vacuum from 10.0 to 13.0psi @15:08:11.86 SS.reconfigure :inhaling 0.91ml/s @15:08:11.91 SS.seek 25ml @15:08:14.17 PBV.dial between PSV and waste1 @15:08:14.54 PSR.select :air @15:08:14.56 PSV.dial PSR @15:08:15.33 PS.reconfigure :fast @15:08:15.38 PS.seek 2.5ml @15:08:39.31 PSV.dial PTV @15:08:40.07 delay 5 seconds @15:08:41.36 Stroke completed -- waiting for pressure to equalize ... @15:08:41.37 SS.reconfigure :exhale @15:08:41.42 SS.hold @15:08:45.15 PSV.dial PSR @15:08:46.41 delay 5 seconds @15:08:51.43 PSV.dial PTV @15:08:52.20 delay 5 seconds @15:08:57.21 PSV.dial :air @15:08:57.98 PS.seek 0ml @15:09:08.83 Intake.select :close @15:09:08.99 SSV.dial :exhaust @15:09:09.75 Exhaust.select :open @15:09:09.87 SS.seek 0ml @15:09:18.83 Exhaust.select :close @15:09:18.94 SSV.dial CBV @15:09:18.98 CBV.dial SSV @15:09:19.74 Allowing 130s for 25.0ml of remaining 825.0ml @15:09:19.75 Intake.select :open @15:09:21.65 washCycleDA #2 of 2 @15:09:21.66 PSR.select :air @15:09:21.68 PSV.dial PSR @15:09:21.92 Intake @15.9psia, vacuum from 10.0 to 13.0psi @15:09:21.94 SS.reconfigure :inhaling 0.91ml/s @15:09:21.99 SS.seek 25ml @15:09:23.09 Pulling 0.5ml of PSR.wash @15:09:23.10 PSR.select :wash @15:09:23.22 PS.reconfigure :default @15:09:23.27 PS.seek 0.5ml @15:09:29.21 delay 5 seconds @15:09:34.23 PSR.select :air @15:09:34.36 PS.seek 2ml @15:09:45.04 Vacuum too high -- waiting up to 40 seconds @15:09:45.06 SS.reconfigure :exhale @15:09:45.12 SS.hold @15:09:50.58 PTV.dial :puck @15:09:50.66 PSV.dial PTV @15:09:51.41 PBV.dial :waste1 @15:09:51.85 PS.seek 1.5ml @15:09:52.75 Intake @15.1psia, vacuum from 9.6 to 12.6psi @15:09:52.77 SS.reconfigure :inhaling 0.73ml/s @15:09:52.82 SS.seek 25ml @15:09:57.74 delay 3 seconds @15:10:00.76 PBV.dial between PSV and waste1 @15:10:00.86 Stroke completed -- waiting for pressure to equalize ... @15:10:00.87 SS.reconfigure :exhale @15:10:00.92 SS.hold @15:10:01.14 PS.seek 0ml @15:10:17.34 delay 2:00 @15:10:21.72 Intake.select :close @15:10:21.88 SSV.dial :exhaust @15:10:22.64 Exhaust.select :open @15:10:22.76 SS.seek 0ml @15:10:31.53 Exhaust.select :close @15:10:31.64 SSV.dial CBV @15:10:31.67 CBV.dial SSV @15:10:32.46 Allowing 130s for 25.0ml of remaining 800.0ml @15:10:32.47 Intake.select :open @15:10:34.64 Intake @15.7psia, vacuum from 10.0 to 13.0psi @15:10:34.66 SS.reconfigure :inhaling 0.73ml/s @15:10:34.71 SS.seek 25ml @15:11:11.40 Stroke completed -- waiting for pressure to equalize ... @15:11:11.42 SS.reconfigure :exhale @15:11:11.47 SS.hold @15:11:36.02 Intake.select :close @15:11:36.18 SSV.dial :exhaust @15:11:37.34 Exhaust.select :open @15:11:37.53 SS.seek 0ml @15:11:46.54 Exhaust.select :close @15:11:46.66 SSV.dial CBV @15:11:46.70 CBV.dial SSV @15:11:47.44 Allowing 130s for 25.0ml of remaining 775.0ml @15:11:47.45 Intake.select :open @15:11:49.63 Intake @15.6psia, vacuum from 10.0 to 13.0psi @15:11:49.65 SS.reconfigure :inhaling 0.73ml/s @15:11:49.69 SS.seek 25ml @15:12:17.36 recoverPSRreagent08 @15:12:17.37 PTV.dial :puck @15:12:17.44 PBV.dial :waste1 @15:12:17.82 delay 30 seconds @15:12:25.67 Stroke completed -- waiting for pressure to equalize ... @15:12:25.68 SS.reconfigure :exhale @15:12:25.73 SS.hold @15:12:47.84 PBV.dial between PSV and waste1 @15:12:48.27 PSR.select :air @15:12:48.33 PSV.dial PSR @15:12:49.10 PS.reconfigure :fast @15:12:49.15 PS.seek 2.5ml @15:12:52.40 Intake.select :close @15:12:52.56 SSV.dial :exhaust @15:12:53.33 Exhaust.select :open @15:12:53.45 SS.seek 0ml @15:13:02.22 Exhaust.select :close @15:13:02.33 SSV.dial CBV @15:13:02.35 CBV.dial SSV @15:13:03.12 Allowing 130s for 25.0ml of remaining 750.0ml @15:13:03.14 Intake.select :open @15:13:05.30 Intake @15.6psia, vacuum from 10.0 to 13.0psi @15:13:05.32 SS.reconfigure :inhaling 0.73ml/s @15:13:05.37 SS.seek 25ml @15:13:13.08 PSV.dial PTV @15:13:13.85 delay 5 seconds @15:13:18.88 PSV.dial PSR @15:13:19.63 delay 5 seconds @15:13:24.65 PSV.dial PTV @15:13:25.55 delay 5 seconds @15:13:30.58 PSV.dial :air @15:13:31.35 PS.seek 0ml @15:13:41.79 Stroke completed -- waiting for pressure to equalize ... @15:13:41.80 SS.reconfigure :exhale @15:13:41.85 SS.hold @15:13:54.83 getPMLReagent08 @15:13:54.84 PRV.dial :air @15:13:55.32 delay 5 seconds @15:14:00.33 PTV.dial :PRVmixing @15:14:01.66 delay 5 seconds @15:14:06.68 PRV.dial :ab2 @15:14:07.14 PTV.dial :PRVmixing @15:14:07.22 PSV.dial :mixloop @15:14:07.70 PS.reconfigure :default @15:14:07.75 PS.seek 0.3ml @15:14:08.50 Intake.select :close @15:14:08.66 SSV.dial :exhaust @15:14:09.43 Exhaust.select :open @15:14:09.54 SS.seek 0ml @15:14:11.68 delay 2 seconds @15:14:13.69 PRV.dial :air @15:14:14.24 PS.seek 0.55ml @15:14:17.58 addPMLReagent08 @15:14:17.60 PSV.dial :air @15:14:18.31 Exhaust.select :close @15:14:18.42 SSV.dial CBV @15:14:18.46 CBV.dial SSV @15:14:18.54 PS.seek 2ml @15:14:19.23 Allowing 130s for 25.0ml of remaining 725.0ml @15:14:19.25 Intake.select :open @15:14:21.41 Intake @15.8psia, vacuum from 10.0 to 13.0psi @15:14:21.43 SS.reconfigure :inhaling 0.73ml/s @15:14:21.48 SS.seek 25ml @15:14:34.21 PSV.dial :PML @15:14:34.68 PTV.dial :mixing @15:14:35.46 PBV.dial :waste1 @15:14:35.89 PS.seek 1.85ml @15:14:38.27 PBV.dial between PSV and waste1 @15:14:38.65 PS.seek 0ml @15:14:51.91 Vacuum too high -- waiting up to 40 seconds @15:14:51.93 SS.reconfigure :exhale @15:14:51.98 SS.hold @15:14:58.33 delay 2 seconds @15:14:59.58 Intake @15.1psia, vacuum from 9.6 to 12.6psi @15:14:59.60 SS.reconfigure :inhaling 0.59ml/s @15:14:59.65 SS.seek 25ml @15:15:00.35 delay 8:00 @15:15:07.37 Stroke completed -- waiting for pressure to equalize ... @15:15:07.38 SS.reconfigure :exhale @15:15:07.43 SS.hold @15:15:26.29 Intake.select :close @15:15:26.45 SSV.dial :exhaust @15:15:27.21 Exhaust.select :open @15:15:27.33 SS.seek 0ml @15:15:36.31 Exhaust.select :close @15:15:36.42 SSV.dial CBV @15:15:36.44 CBV.dial SSV @15:15:37.21 Allowing 130s for 25.0ml of remaining 700.0ml @15:15:37.23 Intake.select :open @15:15:39.86 Intake @15.7psia, vacuum from 10.0 to 13.0psi @15:15:39.88 SS.reconfigure :inhaling 0.59ml/s @15:15:39.93 SS.seek 25ml @15:16:24.32 Stroke completed -- waiting for pressure to equalize ... @15:16:24.33 SS.reconfigure :exhale @15:16:24.38 SS.hold @15:16:48.92 Intake.select :close @15:16:49.54 SSV.dial :exhaust @15:16:50.31 Exhaust.select :open @15:16:50.43 SS.seek 0ml @15:16:59.41 Exhaust.select :close @15:16:59.52 SSV.dial CBV @15:16:59.57 CBV.dial SSV @15:17:00.31 Allowing 130s for 25.0ml of remaining 675.0ml @15:17:00.32 Intake.select :open @15:17:02.49 Intake @15.8psia, vacuum from 10.0 to 13.0psi @15:17:02.51 SS.reconfigure :inhaling 0.59ml/s @15:17:02.56 SS.seek 25ml @15:17:46.96 Stroke completed -- waiting for pressure to equalize ... @15:17:46.97 SS.reconfigure :exhale @15:17:47.02 SS.hold @15:18:13.60 Intake.select :close @15:18:13.76 SSV.dial :exhaust @15:18:14.53 Exhaust.select :open @15:18:14.65 SS.seek 0ml @15:18:23.63 Exhaust.select :close @15:18:23.74 SSV.dial CBV @15:18:23.79 CBV.dial SSV @15:18:24.54 Allowing 130s for 25.0ml of remaining 650.0ml @15:18:24.55 Intake.select :open @15:18:26.72 Intake @15.6psia, vacuum from 10.0 to 13.0psi @15:18:26.74 SS.reconfigure :inhaling 0.59ml/s @15:18:26.79 SS.seek 25ml @15:19:11.30 Stroke completed -- waiting for pressure to equalize ... @15:19:11.31 SS.reconfigure :exhale @15:19:11.36 SS.hold @15:19:40.07 Intake.select :close @15:19:40.23 SSV.dial :exhaust @15:19:40.99 Exhaust.select :open @15:19:41.11 SS.seek 0ml @15:19:49.91 Exhaust.select :close @15:19:50.02 SSV.dial CBV @15:19:50.05 CBV.dial SSV @15:19:50.83 Allowing 130s for 25.0ml of remaining 625.0ml @15:19:50.85 Intake.select :open @15:19:53.02 Intake @15.8psia, vacuum from 10.0 to 13.0psi @15:19:53.04 SS.reconfigure :inhaling 0.59ml/s @15:19:53.09 SS.seek 25ml @15:20:37.75 Vacuum too high -- waiting up to 40 seconds @15:20:37.76 SS.reconfigure :exhale @15:20:37.82 SS.hold @15:20:54.47 Intake @17.5psia, vacuum from 10.0 to 13.0psi @15:20:54.49 SS.reconfigure :inhaling 0.47ml/s @15:20:54.54 SS.seek 25ml @15:20:54.69 Stroke completed -- waiting for pressure to equalize ... @15:20:54.70 SS.reconfigure :exhale @15:20:54.75 SS.hold @15:21:09.67 Intake.select :close @15:21:09.84 SSV.dial :exhaust @15:21:10.62 Exhaust.select :open @15:21:10.74 SS.seek 0ml @15:21:19.58 Exhaust.select :close @15:21:19.70 SSV.dial CBV @15:21:19.72 CBV.dial SSV @15:21:20.52 Allowing 130s for 25.0ml of remaining 600.0ml @15:21:20.53 Intake.select :open @15:21:22.70 Intake @15.6psia, vacuum from 10.0 to 13.0psi @15:21:22.72 SS.reconfigure :inhaling 0.47ml/s @15:21:22.77 SS.seek 25ml @15:22:18.03 Stroke completed -- waiting for pressure to equalize ... @15:22:18.05 SS.reconfigure :exhale @15:22:18.09 SS.hold @15:22:46.73 Intake.select :close @15:22:46.89 SSV.dial :exhaust @15:22:47.67 Exhaust.select :open @15:22:47.79 SS.seek 0ml @15:22:56.86 Exhaust.select :close @15:22:56.97 SSV.dial CBV @15:22:57.01 CBV.dial SSV @15:22:57.77 Allowing 130s for 25.0ml of remaining 575.0ml @15:22:57.78 Intake.select :open @15:22:59.95 Intake @15.9psia, vacuum from 10.0 to 13.0psi @15:22:59.97 SS.reconfigure :inhaling 0.47ml/s @15:23:00.02 SS.seek 25ml @15:23:00.37 recoverPRVreagent08 @15:23:00.38 PTV.dial :mixing @15:23:00.56 PBV.dial :waste1 @15:23:00.93 delay 30 seconds @15:23:30.96 PBV.dial between PSV and waste1 @15:23:31.32 PSV.dial :air @15:23:31.80 PS.reconfigure :fast @15:23:31.85 PS.seek 2.5ml @15:23:55.00 Stroke completed -- waiting for pressure to equalize ... @15:23:55.01 SS.reconfigure :exhale @15:23:55.45 SS.hold @15:23:55.90 PSV.dial :mixloop @15:23:56.40 delay 2 seconds @15:23:58.49 PSV.dial :air @15:23:59.10 delay 2 seconds @15:24:01.18 PSV.dial :mixloop @15:24:01.64 delay 2 seconds @15:24:03.65 PSV.dial :air @15:24:04.13 PS.seek 0ml @15:24:26.65 Intake.select :close @15:24:26.81 SSV.dial :exhaust @15:24:27.58 Exhaust.select :open @15:24:27.64 washCycleDA #1 of 3 @15:24:27.67 PSR.select :air @15:24:27.73 PSV.dial PSR @15:24:27.78 SS.seek 0ml @15:24:29.07 Pulling 0.5ml of PSR.wash @15:24:29.08 PSR.select :wash @15:24:29.20 PS.reconfigure :default @15:24:29.25 PS.seek 0.5ml @15:24:35.16 delay 5 seconds @15:24:36.81 Exhaust.select :close @15:24:36.93 SSV.dial CBV @15:24:36.95 CBV.dial SSV @15:24:37.72 Allowing 130s for 25.0ml of remaining 550.0ml @15:24:37.73 Intake.select :open @15:24:39.90 Intake @15.7psia, vacuum from 10.0 to 13.0psi @15:24:39.92 SS.reconfigure :inhaling 0.47ml/s @15:24:39.97 SS.seek 25ml @15:24:40.18 PSR.select :air @15:24:40.30 PS.seek 2ml @15:24:56.54 PTV.dial :puck @15:24:57.32 PSV.dial PTV @15:24:58.07 PBV.dial :waste1 @15:24:58.49 PS.seek 1.5ml @15:25:04.48 delay 3 seconds @15:25:07.50 PBV.dial between PSV and waste1 @15:25:07.86 PS.seek 0ml @15:25:23.98 delay 2:00 @15:25:35.25 Stroke completed -- waiting for pressure to equalize ... @15:25:35.26 SS.reconfigure :exhale @15:25:35.31 SS.hold @15:26:08.15 Intake.select :close @15:26:08.31 SSV.dial :exhaust @15:26:09.09 Exhaust.select :open @15:26:09.21 SS.seek 0ml @15:26:18.26 Exhaust.select :close @15:26:18.38 SSV.dial CBV @15:26:18.40 CBV.dial SSV @15:26:19.19 Allowing 130s for 25.0ml of remaining 525.0ml @15:26:19.20 Intake.select :open @15:26:21.37 Intake @15.8psia, vacuum from 10.0 to 13.0psi @15:26:21.82 SS.reconfigure :inhaling 0.47ml/s @15:26:21.91 SS.seek 25ml @15:27:16.79 Stroke completed -- waiting for pressure to equalize ... @15:27:16.80 SS.reconfigure :exhale @15:27:16.85 SS.hold @15:27:24.00 recoverPSRreagent08 @15:27:24.01 PTV.dial :puck @15:27:24.19 PBV.dial :waste1 @15:27:24.57 delay 30 seconds @15:27:54.59 PBV.dial between PSV and waste1 @15:27:54.96 PSR.select :air @15:27:54.98 PSV.dial PSR @15:27:55.76 PS.reconfigure :fast @15:27:55.81 PS.seek 2.5ml @15:27:57.04 SS.reconfigure :default @15:27:57.10 SS.hold @15:27:57.17 Intake.select :close @15:27:57.33 ShallowSampler::Clogged in WCR -- Intake @16psia & Outlet @14psia won't equalize after 500.0ml @15:27:57.34 SSV.dial :exhaust @15:27:58.10 Exhaust.select :open @15:27:58.22 SS.reconfigure :exhale @15:27:58.27 SS.seek 0ml @15:28:07.10 Exhaust.select :close @15:28:07.21 Sampled 500.0ml @15:28:07.23 Drying puck before fixing for 5:00 @15:28:07.24 CBV.dial :waste2b @15:28:08.03 delay 1:00 @15:28:19.72 PSV.dial PTV @15:28:20.61 delay 5 seconds @15:28:25.64 PSV.dial PSR @15:28:26.38 delay 5 seconds @15:28:31.40 PSV.dial PTV @15:28:32.16 delay 5 seconds @15:28:37.18 PSV.dial :air @15:28:38.07 PS.seek 0ml @15:29:01.57 washCycleDA #2 of 3 @15:29:01.58 PSR.select :air @15:29:01.60 PSV.dial PSR @15:29:02.94 Pulling 0.5ml of PSR.wash @15:29:02.95 PSR.select :wash @15:29:03.07 PS.reconfigure :default @15:29:03.12 PS.seek 0.5ml @15:29:08.04 CSV.dial :air @15:29:08.51 CS.reconfigure :fast @15:29:08.57 CS.seek 10ml @15:29:09.00 delay 5 seconds @15:29:14.02 PSR.select :air @15:29:14.13 PS.seek 2ml @15:29:30.22 PTV.dial :puck @15:29:30.77 PSV.dial PTV @15:29:31.53 PBV.dial :waste1 @15:29:31.97 PS.seek 1.5ml @15:29:33.29 Pumping wcr puck from tube 6 up to 35psi... @15:29:33.31 CBV.dial SSV @15:29:34.59 CBV.dial between CSV and waste2b @15:29:35.57 CSV.dial between air and CTV @15:29:35.92 CS.seek 7.174ml @15:29:37.85 delay 3 seconds @15:29:40.87 PBV.dial between PSV and waste1 @15:29:41.26 PS.seek 0ml @15:29:43.23 CTV.dial CSV,avoiding: :air @15:29:43.26 CSV.dial CTV @15:29:44.08 CS.reconfigure :pressurize @15:29:44.13 CS.seek 0ml @15:29:57.32 delay 2:00 @15:30:02.57 CBV.dial :waste2b @15:30:02.90 CS.reconfigure :default @15:30:02.95 CS.seek 0ml @15:30:04.16 delay 1:00 @15:31:04.19 CTV.dial :air,avoiding: :intake @15:31:05.53 Fix #1 of 2 (with RNAlater) for 2:30 @15:31:05.54 CSV.dial CSR @15:31:05.97 Pulling 2ml of CSR.RNAlater @15:31:05.99 CSR.select :RNAlater @15:31:06.11 CS.seek 2ml @15:31:12.01 delay 5 seconds @15:31:17.03 CSR.select :air @15:31:17.15 CS.reconfigure :fast @15:31:17.20 CS.seek 10ml @15:31:37.16 Pumping wcr puck from tube 6 up to 35psi... @15:31:37.18 CBV.dial SSV @15:31:38.45 CBV.dial between CSV and waste2b @15:31:39.42 CSV.dial between air and CTV @15:31:40.03 CS.seek 7.11ml @15:31:47.51 CTV.dial CSV,avoiding: :air @15:31:47.53 CSV.dial CTV @15:31:48.93 CS.reconfigure :pressurize @15:31:49.45 CS.seek 0ml @15:31:57.34 recoverPSRreagent08 @15:31:57.35 PTV.dial :puck @15:31:57.42 PBV.dial :waste1 @15:31:57.80 delay 30 seconds @15:32:04.59 delay 2:30 @15:32:27.82 PBV.dial between PSV and waste1 @15:32:28.19 PSR.select :air @15:32:28.21 PSV.dial PSR @15:32:28.97 PS.reconfigure :fast @15:32:29.02 PS.seek 2.5ml @15:32:52.97 PSV.dial PTV @15:32:53.74 delay 5 seconds @15:32:58.76 PSV.dial PSR @15:32:59.53 delay 5 seconds @15:33:04.55 PSV.dial PTV @15:33:05.31 delay 5 seconds @15:33:10.33 PSV.dial :air @15:33:11.10 PS.seek 0ml @15:33:34.22 washCycleDA #3 of 3 @15:33:34.23 PSR.select :air @15:33:34.25 PSV.dial PSR @15:33:35.59 Pulling 0.5ml of PSR.wash @15:33:35.60 PSR.select :wash @15:33:35.72 PS.reconfigure :default @15:33:35.77 PS.seek 0.5ml @15:33:41.75 delay 5 seconds @15:33:46.77 PSR.select :air @15:33:46.89 PS.seek 2ml @15:34:03.03 PTV.dial :puck @15:34:03.11 PSV.dial PTV @15:34:03.86 PBV.dial :waste1 @15:34:04.29 PS.seek 1.5ml @15:34:10.22 delay 3 seconds @15:34:13.24 PBV.dial between PSV and waste1 @15:34:13.61 PS.seek 0ml @15:34:29.75 delay 2:00 @15:34:34.61 CBV.dial :waste2b @15:34:34.96 CS.reconfigure :default @15:34:35.01 CS.seek 0ml @15:34:39.38 delay 1:00 @15:35:39.41 CTV.dial :air,avoiding: :intake @15:35:40.74 Fix #2 of 2 (with RNAlater) for 2:30 @15:35:40.76 CSV.dial CSR @15:35:41.20 Pulling 2ml of CSR.RNAlater @15:35:41.22 CSR.select :RNAlater @15:35:41.34 CS.seek 2ml @15:35:47.50 delay 5 seconds @15:35:52.52 CSR.select :air @15:35:52.64 CS.reconfigure :fast @15:35:52.69 CS.seek 10ml @15:36:12.57 Pumping wcr puck from tube 6 up to 35psi... @15:36:12.58 CBV.dial SSV @15:36:13.84 CBV.dial between CSV and waste2b @15:36:15.23 CSV.dial between air and CTV @15:36:15.87 CS.seek 7.046ml @15:36:23.51 CTV.dial CSV,avoiding: :air @15:36:23.53 CSV.dial CTV @15:36:24.94 CS.reconfigure :pressurize @15:36:25.00 CS.seek 0ml @15:36:29.77 recoverPSRreagent08 @15:36:29.78 PTV.dial :puck @15:36:29.85 PBV.dial :waste1 @15:36:30.23 delay 30 seconds @15:36:42.78 delay 2:30 @15:37:00.26 PBV.dial between PSV and waste1 @15:37:00.62 PSR.select :air @15:37:00.64 PSV.dial PSR @15:37:01.41 PS.reconfigure :fast @15:37:01.46 PS.seek 2.5ml @15:37:25.34 PSV.dial PTV @15:37:26.11 delay 5 seconds @15:37:31.13 PSV.dial PSR @15:37:31.89 delay 5 seconds @15:37:36.91 PSV.dial PTV @15:37:37.79 delay 5 seconds @15:37:42.81 PSV.dial :air @15:37:43.61 PS.seek 0ml @15:38:06.93 Gate.power :camera,:ON @15:38:06.95 Mixing DA Substrates @15:38:06.96 PSR.select :air @15:38:06.97 PSV.dial PSR @15:38:08.37 Pulling 0.25ml of PSR.sub1 @15:38:08.40 PSR.select :sub1 @15:38:08.59 PS.reconfigure :default @15:38:08.72 PS.seek 0.25ml @15:38:12.35 delay 5 seconds @15:38:17.37 Pulling 0.25ml of PSR.sub2 @15:38:17.38 PSR.select :sub2 @15:38:17.50 PS.seek 0.5ml @15:38:20.93 delay 5 seconds @15:38:25.95 PSR.select :air @15:38:26.06 PS.seek 2ml @15:38:42.18 PTV.dial :puck @15:38:42.26 PSV.dial PTV @15:38:43.02 PBV.dial :waste1 @15:38:43.46 PS.seek 1.5ml @15:38:49.37 delay 3 seconds @15:38:52.39 PBV.dial between PSV and waste1 @15:38:52.78 PS.seek 0ml @15:39:08.90 delay 10 seconds @15:39:12.80 CBV.dial :waste2b @15:39:13.13 CS.reconfigure :default @15:39:13.18 CS.seek 0ml @15:39:14.63 delay 1:00 @15:39:18.92 recoverPSRreagent08 @15:39:18.93 PTV.dial :puck @15:39:19.01 PBV.dial :waste1 @15:39:19.38 delay 30 seconds @15:39:49.41 PBV.dial between PSV and waste1 @15:39:49.79 PSR.select :air @15:39:49.81 PSV.dial PSR @15:39:51.08 PS.reconfigure :fast @15:39:51.13 PS.seek 2.5ml @15:40:14.65 CTV.dial :air,avoiding: :intake @15:40:15.40 PSV.dial PTV @15:40:16.18 delay 5 seconds @15:40:21.20 PSV.dial PSR @15:40:21.98 delay 5 seconds @15:40:27.00 PSV.dial PTV @15:40:27.88 delay 5 seconds @15:40:32.90 PSV.dial :air @15:40:33.68 PS.seek 0ml @15:40:57.65 Additional air clear @15:40:57.66 PSR.select :air @15:40:57.68 PSV.dial PSR @15:40:59.05 Pulling 2ml of PSR.air @15:40:59.06 PSR.select :air @15:40:59.09 PS.reconfigure :default @15:40:59.13 PS.seek 2ml @15:41:20.40 delay 5 seconds @15:41:25.42 PTV.dial :puck @15:41:25.50 PSV.dial PTV @15:41:26.27 PBV.dial :waste1 @15:41:26.72 PS.seek 1.5ml @15:41:32.65 delay 3 seconds @15:41:35.67 PBV.dial between PSV and waste1 @15:41:36.04 delay 1 second @15:41:37.06 PSV.dial :waste1 @15:41:38.15 PS.seek 0ml @15:41:54.32 Processing Puck Heater at 28.1C before imaging @15:41:54.33 PH.coast @15:41:54.45 Imaging da puck in Processing Clamp while storing it to tube 5 @15:41:54.63 SC.reconfigure :tight @15:41:54.68 SC.seek 5 @15:41:58.25 SC.configure :limp @15:41:58.47 Elbow.reconfigure :tight @15:41:58.52 Elbow.seek 5 @15:42:00.31 Forearm.reconfigure :tight @15:42:00.36 Forearm.seek 5 + 450 counts @15:42:01.36 SE.reconfigure :pick @15:42:01.42 SE.seek :beamBreak @15:42:03.71 SE.configure :default @15:42:03.87 Elbow.reconfigure :limp @15:42:03.92 Forearm.reconfigure :loose @15:42:03.97 Forearm.seek 5 @15:42:04.22 Elbow.hold @15:42:04.29 Elbow.reconfigure :tight @15:42:04.88 SE.seek 13.13 Pucks below Top Plate @15:42:05.42 Forearm.force -20 @15:42:05.46 Forearm.seek 5 + 308 counts @15:42:05.77 Forearm.reconfigure :tight @15:42:05.82 Forearm.seek 5 + 450 counts @15:42:06.02 SE.seek 14.13 Pucks below Top Plate @15:42:06.62 Forearm.reconfigure :loose @15:42:06.67 Forearm.seek :retract @15:42:07.60 Elbow.seek PC @15:42:10.12 Elbow.reconfigure :loose @15:42:10.22 Forearm.seek PC @15:42:11.16 Hand.close @15:42:11.79 PC.seek :open @15:42:16.58 Forearm.seek :retract @15:42:17.54 Elbow.reconfigure :tight @15:42:17.59 Elbow.seek 5 @15:42:20.20 Forearm.reconfigure :tight @15:42:20.25 Forearm.seek 5 + 450 counts @15:42:21.22 Hand.open @15:42:21.85 SE.seek 13.78 Pucks below Top Plate @15:42:22.13 SE.reconfigure :seal @15:42:22.18 SE.seek 12.82 Pucks below Top Plate @15:42:22.71 Lights, camera, ACTION! @15:42:22.74 PuckCamera: snap -bin=2,2 -auto /var/log/esp/da23dec1115h1000ml.tif @15:42:42.24 Waiting >20 seconds for da to relinquish FlushPuck @15:42:44.55 SX HX9/16 (USB:24): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 18.464 seconds /var/log/esp/da23dec1115h1000ml.tif: TIFF Upload Complete @15:42:44.57 PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da23dec1115h1000ml15s.tif @15:43:00.29 SX HX9/16 (USB:24): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15 seconds /var/log/esp/midres/da23dec1115h1000ml15s.tif: TIFF Upload Complete @15:43:00.32 PuckCamera: snap -bin=2,2 20.0 /var/log/esp/midres/da23dec1115h1000ml20s.tif @15:43:20.95 SX HX9/16 (USB:24): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 20 seconds /var/log/esp/midres/da23dec1115h1000ml20s.tif: TIFF Upload Complete @15:43:21.25 Gate.power :camera,:OFF @15:43:21.26 DAfans.email "18.464 sec auto-exposure",Subject: "da23dec1115h1000ml images!" @15:43:21.70 Elbow.seek 5 @15:43:21.92 Forearm.seek 5 + 450 counts @15:43:22.01 SE.reconfigure :gently @15:43:22.63 SE.seek 13.53 Pucks below Top Plate @15:43:23.33 Elbow.reconfigure :limp @15:43:23.39 Forearm.reconfigure :loose @15:43:23.44 Forearm.seek 5 @15:43:23.83 Elbow.hold @15:43:23.91 Elbow.reconfigure :tight @15:43:23.97 SE.reconfigure :default @15:43:24.02 SE.seek 13.72 Pucks below Top Plate @15:43:24.38 Forearm.force -20 @15:43:24.43 Forearm.seek 5 + 247 counts @15:43:24.90 Forearm.reconfigure :tight @15:43:24.95 Forearm.seek 5 + 450 counts @15:43:25.39 SE.seek :down @15:43:26.67 Forearm.reconfigure :loose @15:43:26.77 Forearm.seek :retract @15:43:27.25 SE.stop @15:43:27.96 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @15:43:28.50 Elbow.seek FlushPuck::Garage @15:43:30.54 Elbow.reconfigure :loose @15:43:30.85 Forearm.seek :garage @15:43:31.95 Hand.close @15:43:32.89 Forearm.seek :retract @15:43:33.94 Elbow.reconfigure :tight @15:43:34.04 Elbow.seek PC @15:43:35.41 Elbow.reconfigure :loose @15:43:35.63 Forearm.reconfigure :tight @15:43:35.72 Forearm.seek PC @15:43:38.35 PC.seek :closed @15:43:43.19 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.66 @15:43:43.39 Hand.open @15:43:44.19 Forearm.reconfigure :loose @15:43:44.28 Forearm.seek :retract @15:43:45.32 PSR.select :air @15:43:45.38 Storing wcr puck in Collection Clamp to tube 5 @15:43:45.42 PSV.dial PSR @15:43:45.93 SC.reconfigure :tight @15:43:46.03 SC.seek 5 @15:43:46.19 Pulling 0.5ml of PSR.flush @15:43:46.22 PSR.select :flush @15:43:46.40 SC.configure :limp @15:43:46.71 PS.seek 0.5ml @15:43:46.97 Elbow.reconfigure :tight @15:43:47.07 Elbow.seek 5 @15:43:50.01 Forearm.reconfigure :tight @15:43:50.11 Forearm.seek 5 + 450 counts @15:43:51.28 SE.reconfigure :pick @15:43:51.40 SE.seek :beamBreak @15:43:52.69 delay 5 seconds @15:43:53.68 SE.configure :default @15:43:54.00 Elbow.reconfigure :limp @15:43:54.10 Forearm.reconfigure :loose @15:43:54.19 Forearm.seek 5 @15:43:54.54 Elbow.hold @15:43:54.67 Elbow.reconfigure :tight @15:43:55.82 SE.seek 14.15 Pucks below Top Plate @15:43:56.50 Forearm.force -20 @15:43:56.64 Forearm.seek 5 + 255 counts @15:43:57.08 Forearm.reconfigure :tight @15:43:57.16 Forearm.seek 5 + 450 counts @15:43:57.40 SE.seek 15.15 Pucks below Top Plate @15:43:57.63 upsync @15:43:57.72 PSR.select :air @15:43:57.94 PS.seek 2.5ml @15:43:58.01 Forearm.reconfigure :loose @15:43:58.07 Forearm.seek :retract @15:43:59.01 Elbow.seek CC @15:44:00.61 Elbow.reconfigure :loose @15:44:00.71 Forearm.seek CC @15:44:01.74 Hand.close @15:44:02.41 CC.seek :open @15:44:07.17 Forearm.seek :retract @15:44:08.16 Elbow.reconfigure :tight @15:44:08.21 Elbow.seek 5 @15:44:10.04 Elbow.reconfigure :limp @15:44:10.09 Forearm.seek 5 @15:44:11.11 Elbow.hold @15:44:11.18 Elbow.reconfigure :tight @15:44:11.23 Hand.open @15:44:12.04 Forearm.force -20 @15:44:12.46 Forearm.seek 5 + 320 counts @15:44:12.83 Forearm.reconfigure :tight @15:44:12.88 Forearm.seek 5 + 450 counts @15:44:13.18 SE.seek :down @15:44:14.32 Forearm.reconfigure :loose @15:44:14.37 Forearm.seek :retract @15:44:14.86 SE.stop @15:44:15.37
Starting finalPurge @15:44:19.24 delay 1 second @15:44:20.26 PRV.dial :waste2,avoiding: :ab2 @15:44:22.13 PTV.dial :PRVmixing @15:44:23.44 PSV.dial :mixloop @15:44:24.61 PS.seek :empty @15:44:35.08
Waiting >20 seconds for psFlush to relinquish FlushPuck @15:44:51.05 PSR.select :air @15:44:51.07 PSV.dial PSR @15:44:52.09 Pulling 0.5ml of PSR.flush @15:44:52.11 PSR.select :flush @15:44:52.23 PS.seek 0.5ml @15:44:58.17 delay 5 seconds @15:45:03.19 PSR.select :air @15:45:03.31 PS.seek 2.5ml @15:45:24.58 delay 1 second @15:45:25.60 PBV.dial :waste1 @15:45:25.95 PTV.dial :mixing @15:45:26.70 PSV.dial :mixloop @15:45:27.75 PS.seek :empty @15:45:54.33 PSV.dial PSR @15:45:55.37 PSR.select :flush @15:45:55.50 PS.seek 1ml @15:46:06.51 PSR.select :air @15:46:06.63 PS.seek 2.5ml @15:46:22.73 PSV.dial PTV @15:46:23.50 PTV.dial :puck @15:46:24.25 PBV.dial :waste1 @15:46:24.33 PS.seek 0ml @15:46:50.73 PSV.dial PSR @15:46:51.50 PSR.select :flush @15:46:51.62 PS.seek 0.5ml @15:46:57.64 PSR.select :air @15:46:57.76 PS.seek 2.5ml @15:47:19.07 PSV.dial PTV @15:47:19.84 PBV.dial PSV @15:47:20.85 PS.seek 0ml @15:47:47.20 PSV.dial PBV @15:47:47.68 PS.seek 2.5ml @15:48:13.95 PSV.dial :waste1 @15:48:14.71 PS.seek 0ml @15:48:41.01 PSV.dial PSR @15:48:41.48 PSR.select :air @15:48:41.51 PS.seek 2.5ml @15:49:07.89 PSV.dial PTV @15:49:08.62 PBV.dial PSV @15:49:08.71 PS.seek 0ml @15:49:35.07 PSV.dial PBV @15:49:35.55 PS.seek 2.5ml @15:50:02.10 PSV.dial :waste1 @15:50:02.85 PS.seek 0ml @15:50:29.20 PSV.dial PSR @15:50:29.67 PSR.select :flush @15:50:29.79 PS.seek 1ml @15:50:40.79 PSR.select :air @15:50:40.91 PS.seek 2.5ml @15:50:57.07 PSV.dial PTV @15:50:57.81 PTV.dial :puck @15:50:57.89 PBV.dial :waste1 @15:50:58.45 PS.seek 0ml @15:51:24.82 PSV.dial PSR @15:51:25.57 PSR.select :flush @15:51:25.70 PS.seek 0.5ml @15:51:31.60 PSR.select :air @15:51:31.72 PS.seek 2.5ml @15:51:53.01 PSV.dial PTV @15:51:53.88 PBV.dial PSV @15:51:54.45 PS.seek 0ml @15:52:20.79 PSV.dial PBV @15:52:21.25 PS.seek 2.5ml @15:52:47.63 PSV.dial :waste1 @15:52:48.37 PS.seek 0ml @15:53:14.69 PSV.dial PSR @15:53:15.16 PSR.select :air @15:53:15.19 PS.seek 2.5ml @15:53:41.57 PSV.dial PTV @15:53:42.30 PBV.dial PSV @15:53:42.50 PS.seek 0ml @15:54:08.82 PSV.dial PBV @15:54:09.36 PS.seek 2.5ml @15:54:35.76 PSV.dial :waste1 @15:54:36.50 PS.seek 0ml @15:55:03.04
Loading FlushPuck into Collection Clamp from Processing Clamp @15:55:03.23 Elbow.seek PC @15:55:05.72 Elbow.reconfigure :loose @15:55:05.82 Forearm.seek PC @15:55:06.76 Hand.close @15:55:07.40 PC.seek :open @15:55:12.12 Forearm.seek :retract @15:55:13.07 Elbow.reconfigure :tight @15:55:13.12 Elbow.seek CC @15:55:16.75 Elbow.reconfigure :loose @15:55:16.85 Forearm.reconfigure :tight @15:55:16.90 Forearm.seek CC @15:55:18.10 CC.seek :closed @15:55:22.62 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.39 @15:55:22.68 Hand.open @15:55:23.33 Forearm.reconfigure :loose @15:55:23.38 Forearm.seek :retract @15:55:24.37 Flushing the Collection Valve::Manifold @15:55:24.50 CSV.dial CSR @15:55:24.95 Pulling 5ml of CSR.flush @15:55:24.96 CSR.select :flush @15:55:25.08 CS.seek 5ml @15:55:38.67 delay 5 seconds @15:55:43.69 CSR.select :air @15:55:43.86 CS.reconfigure :fast @15:55:43.91 CS.seek 7.501ml @15:55:51.08 CSV.dial :waste @15:55:52.38 CS.reconfigure :default @15:55:52.43 CS.seek 0ml @15:56:12.51 Flushing lower manifold's waste1 port @15:56:12.69 CSV.dial :flush @15:56:13.14 CS.reconfigure :fast @15:56:13.19 CS.seek 5ml @15:56:26.17 delay 5 seconds @15:56:31.19 CSV.dial :air @15:56:31.70 CS.seek 7ml @15:56:37.36 CSV.dial CTV @15:56:37.38
CTV.dial CSV @15:56:38.76 CBV.dial :waste1 @15:56:39.55 CS.seek 0ml @15:56:56.39 Flushing lower manifold's waste2 port @15:56:56.52 CSV.dial :flush @15:56:57.38 CS.seek 5ml @15:57:10.36 delay 5 seconds @15:57:15.38 CSV.dial :air @15:57:15.89 CS.seek 7ml @15:57:21.51 CSV.dial CTV @15:57:21.54
CTV.dial CSV @15:57:22.00 CBV.dial :waste2 @15:57:23.05 CS.seek 0ml @15:57:39.70 Flushing lower manifold's waste2b port @15:57:39.82 CSV.dial :flush @15:57:40.57 CS.seek 5ml @15:57:53.26 delay 5 seconds @15:57:58.28 CSV.dial :air @15:57:58.81 CS.seek 7ml @15:58:04.45 CSV.dial CTV @15:58:04.47
CTV.dial CSV @15:58:04.92 CBV.dial :waste2b @15:58:05.97 CS.seek 0ml @15:58:22.61 Disinfecting external Intake with kill @15:58:22.73 CSV.dial CSR @15:58:23.29 Pulling 7.999ml of CSR.kill @15:58:23.30 CSR.select :kill @15:58:23.42 CS.seek 8ml @15:58:43.35 delay 5 seconds @15:58:48.37 CSR.select :air @15:58:48.49 CS.seek 10ml @15:58:54.14 CSV.dial CBV @15:58:54.16
CBV.dial CSV @15:58:55.26 CTV.dial :intake @15:58:56.11 Intake.select :open @15:58:56.22 CS.seek 2ml @15:59:15.16 Intake.select :close @15:59:15.27 CTV.dial :air @15:59:16.05 CS.reconfigure :default @15:59:16.10 CS.seek 0ml @15:59:22.04 CSV.dial CSR @15:59:23.07 Pulling 5ml of CSR.flush @15:59:23.08 CSR.select :flush @15:59:23.20 CS.seek 5ml @15:59:36.98 delay 5 seconds @15:59:42.00 CSR.select :air @15:59:42.11 CS.seek 10ml @15:59:55.66 CSV.dial CBV @15:59:56.69 CS.seek 0ml @16:00:23.04 Garaging FlushPuck in Collection Clamp @16:00:23.11 Elbow.reconfigure :tight @16:00:23.16 Elbow.seek CC @16:00:23.42 Elbow.reconfigure :loose @16:00:23.52 Forearm.seek CC @16:00:24.57 Hand.close @16:00:25.15 CC.seek :open @16:00:29.81 Forearm.seek :retract @16:00:30.80 Elbow.reconfigure :tight @16:00:30.85 Elbow.seek :garage @16:00:33.72 Elbow.reconfigure :loose @16:00:33.82 Forearm.reconfigure :tight @16:00:33.87 Forearm.seek :garage @16:00:34.89 Hand.open @16:00:35.58 Forearm.reconfigure :loose @16:00:35.63 Forearm.seek :retract @16:00:36.62 Completed [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] begun 13:29:24 @16:00:36.67 Asleep after completing DA_WCR_121123 assays @16:00:36.69 GoodNews.email "{1 => 33,\n 2 => 33,\n 3 => 33,\n 4 => 33,\n 5 => 16,\n 6 => 17,\n 7 => 31}\nCTD data unavailable\nCan@15:58:34, 31% flow, 16.4C, 33% humidity, 15.1psia, 13.661V, 0.496A, 200.084Ah, 6.77W\nSampled 500.0ml",Subject: "Asleep after completing DA_WCR_121123 assays" @16:00:41.37 upsync + ESPwhich DA_WCR_121123b.rb + fn=DA_WCR_121123b.rb + IFS=: + set /home/esp/esp2/mission . + fullPath=/home/esp/esp2/mission/DA_WCR_121123b.rb + [ -f /home/esp/esp2/mission/DA_WCR_121123b.rb ] + realpath /home/esp/esp2/mission/DA_WCR_121123b.rb + return 0 + scriptPath=/home/esp/esp2local/mission/DA_WCR_121123b.rb + exec ruby /home/esp/esp2/lib/esp.rb /home/esp/esp2local/mission/DA_WCR_121123b.rb @21:02:39.22UTC11-Dec-23
ESP2 v3.42 I2C Gateway v3.20 for core bus @21:02:41.88 Configuration for short puck jake 1/11/21 by brent@mbari.org /home/esp/esp2/type/shallow/jake/configure.rb @21:02:45.57 Polling Can every 10:00 @21:02:45.73 Cleanly exited at 19:19:31UTC11-Dec-23 @21:02:45.78 Loading /home/esp/esp2local/mission/DA_WCR_121123b.rb mission @21:02:45.83 Preparing to sleep until + 00:00:05 @21:02:45.85 GoodNews.email "Starting DA_WCR_121123b w/pucks in tube 6\n{1 => 33,\n 2 => 33,\n 3 => 33,\n 4 => 33,\n 5 => 16,\n 6 => 17,\n 7 => 31}\nCTD data unavailable\nCan@21:02:45, 31% flow, 16.6C, 33% humidity, 15.1psia, 13.715V, 0.339A, 201.538Ah, 4.65W",Subject: "DA_WCR_121123b begun" @21:02:45.89
Asleep til + 00:00:05 @21:02:45.94 Continuing at 21:02 @21:02:46.18 Gate.power :core,:OFF @21:02:46.19 Gate.power :raw,:OFF @21:02:46.20 Shutdown @21:02:49.41 GoodNews.email "Then, in tube 6: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_121123b.rb:14\n{1 => 33,\n 2 => 33,\n 3 => 33,\n 4 => 33,\n 5 => 16,\n 6 => 17,\n 7 => 31}\nCTD data unavailable\nCan@21:02:45, 31% flow, 16.7C, 33% humidity, 15.1psia, 13.715V, 0.327A, 201.538Ah, 4.48W",Subject: "Asleep until 21:02" @21:02:50.81 upsync @21:02:50.85
Powering core bus @21:02:53.82 upsync @21:02:56.45
Gate.power :raw,:ON @21:02:57.47 CS.configure :default @21:02:57.52 PS.configure :default @21:02:57.57 SS.configure :default @21:02:57.62 CC.configure :default @21:02:57.67 PC.configure :default @21:02:57.73 Forearm.configure :loose @21:02:57.78 Elbow.configure :loose @21:02:57.85 SC.configure :loose @21:02:57.90 SE.configure :default @21:02:57.94 CH.configure :default @21:02:57.98 PH.configure :default @21:02:58.02 All dwarf controllers running firmware version 3.85 @21:02:58.13 Valve.reset @21:02:58.39 Intake.select :close @21:02:58.44 Exhaust.select :close @21:02:58.45 CTV.dial :air @21:02:58.53 PSV.dial :air @21:02:58.59 CSV.dial :waste1 @21:02:58.71
Forearm.home.seek :retract @21:02:59.07 SSV.dial :spit,avoiding: :exhaust @21:02:59.50 Hand.close @21:02:59.53
Elbow.reconfigure :loose @21:02:59.63 Elbow.jog 1500 @21:02:59.81 PS.home.seek 0ml @21:03:00.09
Elbow.reconfigure :loose @21:03:00.14 Elbow.home.seek :clear @21:03:01.05 CS.reconfigure :fast @21:03:01.09 SS.home.seek 0ml @21:03:01.18 CS.home.seek 0ml @21:03:03.49
SE.home.seek :down @21:03:04.12 SC.reconfigure :tight @21:03:04.17 SC.home.seek :initial @21:03:08.13 PS.stop @21:03:26.29
SC.configure :limp @21:03:26.34 Forearm.seek :retract @21:03:26.41 Elbow.seek :hammer @21:03:27.44 Forearm.reconfigure :tight @21:03:27.49 Forearm.seek retracted @21:03:28.29 PC.home.seek :open @21:03:28.30
CC.home.seek :open @21:03:31.71 Phase [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] @21:03:31.72 Notice.email "At 21:03:31 began in tube 6: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_121123b.rb:14\n{1 => 33,\n 2 => 33,\n 3 => 33,\n 4 => 33,\n 5 => 16,\n 6 => 17,\n 7 => 31}\nCTD data unavailable\nCan@21:03:31, 31% flow, 16.6C, 33% humidity, 15.1psia, 13.744V, 0.290A, 201.543Ah, 3.99W",Subject: "Started DA_WCR_121123b phase initialPurge; da [1000, 1000] #da and wsr are run simultanelously" @21:03:31.75
Starting initialPurge @21:03:31.76 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @21:03:31.83 Hand.open @21:03:32.56 Elbow.reconfigure :tight @21:03:32.61 Elbow.seek FlushPuck::Garage @21:03:34.52 Elbow.reconfigure :loose @21:03:34.62 Forearm.reconfigure :loose @21:03:34.67 Forearm.seek :garage @21:03:35.61 Hand.close @21:03:36.31 Forearm.seek :retract @21:03:36.37 upsync @21:03:37.28
Elbow.reconfigure :tight @21:03:37.33 Elbow.seek CC @21:03:40.31 Elbow.reconfigure :loose @21:03:40.41 Forearm.reconfigure :tight @21:03:40.47 Forearm.seek CC @21:03:41.78 CC.seek :closed @21:03:46.32 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.41 @21:03:46.38 Hand.open @21:03:47.11 Forearm.reconfigure :loose @21:03:47.15 Forearm.seek :retract @21:03:48.12 Flushing the Collection Valve::Manifold @21:03:48.25 CSV.dial CSR @21:03:49.54 Pulling 5ml of CSR.flush @21:03:49.55 CSR.select :flush @21:03:49.66 CS.reconfigure :default @21:03:49.71 CS.seek 5ml @21:04:03.71 delay 5 seconds @21:04:08.73 CSR.select :air @21:04:08.90 CS.reconfigure :fast @21:04:08.95 CS.seek 7.5ml @21:04:15.90 CSV.dial :waste @21:04:17.20 CS.reconfigure :default @21:04:17.25 CS.seek 0ml @21:04:37.31 Purging external Intake @21:04:37.43 CSV.dial :flush @21:04:38.00 CS.reconfigure :fast @21:04:38.05 CS.seek 10ml @21:05:02.59 delay 5 seconds @21:05:07.61 CSV.dial CBV @21:05:07.64
CBV.dial CSV @21:05:08.38 CTV.dial :intake @21:05:09.16 Intake.select :open @21:05:09.28 CS.seek 0ml @21:05:32.99 Intake.select :close @21:05:33.10 CTV.dial :air @21:05:33.90 Flushing upper manifold @21:05:34.02 CSV.dial :flush @21:05:34.78 CS.seek 5ml @21:05:47.46 delay 5 seconds @21:05:52.48 CSV.dial :air @21:05:52.99 CS.seek 7ml @21:05:58.49 CSV.dial CBV @21:05:58.51
CBV.dial CSV @21:05:59.56 CTV.dial :air @21:05:59.64 CS.seek 0ml @21:06:16.27 delay 7 seconds @21:06:23.30 CS.seek 3ml @21:06:31.40 CSV.dial :waste @21:06:31.86 CS.seek 0ml @21:06:39.73 CSV.dial :flush @21:06:40.19 CS.seek 3ml @21:06:48.21 delay 5 seconds @21:06:53.23 CSV.dial :air @21:06:53.74 CS.seek 5ml @21:06:59.46 CSV.dial :air @21:06:59.54 CS.seek 0ml @21:07:11.71 delay 7 seconds @21:07:18.74 CS.seek 3ml @21:07:26.99 CSV.dial :waste @21:07:27.73 CS.seek 0ml @21:07:35.37 Garaging FlushPuck in Collection Clamp @21:07:35.44 Elbow.reconfigure :tight @21:07:35.49 Elbow.seek CC @21:07:35.77 Elbow.reconfigure :loose @21:07:35.87 Forearm.seek CC @21:07:36.90 Hand.close @21:07:37.55 CC.seek :open @21:07:42.60 Forearm.seek :retract @21:07:43.62 Elbow.reconfigure :tight @21:07:43.67 Elbow.seek :garage @21:07:46.55 Elbow.reconfigure :loose @21:07:46.65 Forearm.reconfigure :tight @21:07:46.70 Forearm.seek :garage @21:07:47.71 Hand.open @21:07:48.35 Forearm.reconfigure :loose @21:07:48.40 Forearm.seek :retract @21:07:49.37 da sampling at most 1000ml, wcr at most 1000ml @21:07:49.41 DAfans.email "Started da in tube 6 at 21:07:49UTC11-Dec-23\n{1 => 33,\n 2 => 33,\n 3 => 33,\n 4 => 33,\n 5 => 16,\n 6 => 17,\n 7 => 31}\nCTD data unavailable\nCan@21:03:31, 31% flow, 16.6C, 33% humidity, 15.1psia, 13.744V, 0.290A, 201.543Ah, 3.99W",Subject: "da sampling at most 1000ml, wcr at most 1000ml" @21:07:49.44
Loading sh1 puck from tube 6 into Collection Clamp @21:07:49.70 SC.reconfigure :tight @21:07:49.75 SC.seek 6 @21:07:53.78 upsync @21:07:56.41
SC.configure :limp @21:07:56.65 Elbow.reconfigure :tight @21:07:56.70 Elbow.seek 6 @21:07:58.64 Forearm.reconfigure :tight @21:07:58.69 Forearm.seek 6 + 450 counts @21:07:59.72 SE.reconfigure :pick @21:07:59.78 SE.seek :beamBreak @21:08:01.84 SE.configure :default @21:08:02.00 Elbow.seek 6 @21:08:02.19 Forearm.seek 6 + 300 counts @21:08:02.43 Forearm.reconfigure :loose @21:08:02.48 Hand.close @21:08:03.06 SE.seek :down @21:08:03.26
Forearm.seek :retract @21:08:04.66 Elbow.seek CC @21:08:04.75 SE.stop @21:08:06.29
Elbow.reconfigure :loose @21:08:06.39 Forearm.reconfigure :tight @21:08:06.44 Forearm.seek CC @21:08:07.69 CC.seek :closed @21:08:12.22 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.37 @21:08:12.23 Hand.open @21:08:12.85 Forearm.reconfigure :loose @21:08:12.90 Forearm.seek :retract @21:08:13.89 collectSample 1000ml w/bubble point of 24psi @21:08:13.90 Priming 7ml @21:08:13.91 Intake.select :close @21:08:13.92 Exhaust.select :close @21:08:13.93 SS.hold @21:08:14.15 Calibrating pressure and temperature sensors @21:08:14.17 CTV.dial :air @21:08:14.26 SSV.dial :spit,avoiding: :exhaust @21:08:14.28 SS.reconfigure :fast @21:08:14.32 SS.seek 5ml @21:08:17.22 SSV.dial CBV @21:08:17.24
CBV.dial SSV @21:08:18.63 delay 3.8 seconds @21:08:22.56 Sensor limits: Intake max 100.5psia; Outlet max 93.6psia @21:08:22.57 SSV.dial :spit @21:08:23.88 SS.seek 0ml @21:08:26.89 SSV.dial CBV @21:08:28.21 CTV.dial :intake @21:08:29.01 SSV.dial CBV @21:08:29.03
CBV.dial SSV @21:08:29.11 Allowing 40s for 7.0ml of remaining 7.0ml @21:08:29.12 Intake.select :open @21:08:31.29 Intake @14.3psia, vacuum from 8.8 to 11.8psi @21:08:31.31 SS.reconfigure :inhaling 2.20ml/s @21:08:31.35 SS.seek 7ml @21:08:37.81 Stroke completed -- waiting for pressure to equalize ... @21:08:37.82 SS.reconfigure :exhale @21:08:37.87 SS.hold @21:08:47.97 Intake.select :close @21:08:48.13 SSV.dial :exhaust @21:08:48.89 Exhaust.select :open @21:08:49.01 SS.seek 0ml @21:08:53.19 Exhaust.select :close @21:08:53.31 Sampling 1000.0ml @21:08:53.32 Intake.select :close @21:08:53.33 Exhaust.select :close @21:08:53.34 SS.reconfigure :default @21:08:53.38 SS.hold @21:08:53.58 CTV.dial :intake @21:08:53.76 SSV.dial CBV @21:08:53.78
CBV.dial SSV @21:08:54.56 Allowing 130s for 25.0ml of remaining 1000.0ml @21:08:54.57 Intake.select :open @21:08:56.74 Intake @15.1psia, vacuum from 9.6 to 12.6psi @21:08:56.76 SS.reconfigure :inhaling 2.20ml/s @21:08:56.80 SS.seek 25ml @21:09:05.59 Vacuum too high -- waiting up to 40 seconds @21:09:05.60 SS.reconfigure :exhale @21:09:05.65 SS.hold @21:09:11.72 Intake @15.6psia, vacuum from 10.0 to 13.0psi @21:09:11.73 SS.reconfigure :inhaling 1.76ml/s @21:09:11.78 SS.seek 25ml @21:09:20.09 Vacuum too high -- waiting up to 40 seconds @21:09:20.10 SS.reconfigure :exhale @21:09:20.15 SS.hold @21:09:26.77 Intake @16.5psia, vacuum from 10.0 to 13.0psi @21:09:26.78 SS.reconfigure :inhaling 1.41ml/s @21:09:26.83 SS.seek 25ml @21:09:27.45 Stroke completed -- waiting for pressure to equalize ... @21:09:27.46 SS.reconfigure :exhale @21:09:27.51 SS.hold @21:09:51.94 Intake.select :close @21:09:52.09 SSV.dial :exhaust @21:09:52.87 Exhaust.select :open @21:09:52.98 SS.seek 0ml @21:10:01.83 Exhaust.select :close @21:10:01.93 SSV.dial CBV @21:10:01.95
CBV.dial SSV @21:10:02.74 Allowing 130s for 25.0ml of remaining 975.0ml @21:10:02.75 Intake.select :open @21:10:04.92 Intake @15.8psia, vacuum from 10.0 to 13.0psi @21:10:04.93 SS.reconfigure :inhaling 1.41ml/s @21:10:04.98 SS.seek 25ml @21:10:18.49 Vacuum too high -- waiting up to 40 seconds @21:10:18.50 SS.reconfigure :exhale @21:10:18.55 SS.hold @21:10:26.19 Intake @20.1psia, vacuum from 10.0 to 13.0psi @21:10:26.21 SS.reconfigure :inhaling 1.13ml/s @21:10:26.26 SS.seek 25ml @21:10:29.12 Vacuum too high -- waiting up to 40 seconds @21:10:29.14 SS.reconfigure :exhale @21:10:29.19 SS.hold @21:10:29.65 Intake @17.5psia, vacuum from 10.0 to 13.0psi @21:10:29.67 SS.reconfigure :inhaling 0.91ml/s @21:10:29.71 SS.seek 25ml @21:10:39.09 Stroke completed -- waiting for pressure to equalize ... @21:10:39.10 SS.reconfigure :exhale @21:10:39.15 SS.hold @21:11:07.64 Intake.select :close @21:11:07.80 SSV.dial :exhaust @21:11:08.56 Exhaust.select :open @21:11:08.68 SS.seek 0ml @21:11:17.95 Exhaust.select :close @21:11:18.06 SSV.dial CBV @21:11:18.08
CBV.dial SSV @21:11:18.87 Allowing 130s for 25.0ml of remaining 950.0ml @21:11:18.88 Intake.select :open @21:11:21.05 Intake @15.6psia, vacuum from 10.0 to 13.0psi @21:11:21.07 SS.reconfigure :inhaling 0.91ml/s @21:11:21.12 SS.seek 25ml @21:11:50.96 Stroke completed -- waiting for pressure to equalize ... @21:11:50.97 SS.reconfigure :exhale @21:11:51.02 SS.hold @21:12:19.63 Intake.select :close @21:12:19.78 SSV.dial :exhaust @21:12:20.56 Exhaust.select :open @21:12:21.10 SS.seek 0ml @21:12:29.93 Exhaust.select :close @21:12:30.04 SSV.dial CBV @21:12:30.07
CBV.dial SSV @21:12:30.84 Allowing 130s for 25.0ml of remaining 925.0ml @21:12:30.85 Intake.select :open @21:12:33.02 Intake @15.8psia, vacuum from 10.0 to 13.0psi @21:12:33.04 SS.reconfigure :inhaling 0.91ml/s @21:12:33.08 SS.seek 25ml @21:13:03.12 Stroke completed -- waiting for pressure to equalize ... @21:13:03.13 SS.reconfigure :exhale @21:13:03.18 SS.hold @21:13:31.75 Intake.select :close @21:13:31.91 SSV.dial :exhaust @21:13:32.74 Exhaust.select :open @21:13:32.85 SS.seek 0ml @21:13:41.57 Exhaust.select :close @21:13:41.68 SSV.dial CBV @21:13:41.71
CBV.dial SSV @21:13:42.48 Allowing 130s for 25.0ml of remaining 900.0ml @21:13:42.49 Intake.select :open @21:13:44.66 Intake @15.6psia, vacuum from 10.0 to 13.0psi @21:13:44.68 SS.reconfigure :inhaling 0.91ml/s @21:13:44.72 SS.seek 25ml @21:14:14.62 Stroke completed -- waiting for pressure to equalize ... @21:14:14.63 SS.reconfigure :exhale @21:14:14.68 SS.hold @21:14:41.22 Intake.select :close @21:14:41.37 SSV.dial :exhaust @21:14:42.14 Exhaust.select :open @21:14:42.68 SS.seek 0ml @21:14:51.35 Exhaust.select :close @21:14:51.46 SSV.dial CBV @21:14:51.50
CBV.dial SSV @21:14:52.24 Allowing 130s for 25.0ml of remaining 875.0ml @21:14:52.25 Intake.select :open @21:14:54.42 Intake @15.6psia, vacuum from 10.0 to 13.0psi @21:14:54.44 SS.reconfigure :inhaling 0.91ml/s @21:14:54.49 SS.seek 25ml @21:15:24.43 Stroke completed -- waiting for pressure to equalize ... @21:15:24.44 SS.reconfigure :exhale @21:15:24.49 SS.hold @21:15:48.97 Intake.select :close @21:15:49.13 SSV.dial :exhaust @21:15:49.88 Exhaust.select :open @21:15:50.00 SS.seek 0ml @21:15:58.96 Exhaust.select :close @21:15:59.07 SSV.dial CBV @21:15:59.09
CBV.dial SSV @21:16:00.32 Allowing 130s for 25.0ml of remaining 850.0ml @21:16:00.33 Intake.select :open @21:16:02.50 Intake @15.7psia, vacuum from 10.0 to 13.0psi @21:16:02.52 SS.reconfigure :inhaling 0.91ml/s @21:16:02.56 SS.seek 25ml @21:16:32.01 Stroke completed -- waiting for pressure to equalize ... @21:16:32.02 SS.reconfigure :exhale @21:16:32.06 SS.hold @21:16:58.61 Intake.select :close @21:16:58.76 SSV.dial :exhaust @21:16:59.55 Exhaust.select :open @21:16:59.66 SS.seek 0ml @21:17:08.41 Exhaust.select :close @21:17:08.52 SSV.dial CBV @21:17:08.54
CBV.dial SSV @21:17:09.33 Allowing 130s for 25.0ml of remaining 825.0ml @21:17:09.35 Intake.select :open @21:17:11.52 Intake @15.6psia, vacuum from 10.0 to 13.0psi @21:17:11.54 SS.reconfigure :inhaling 0.91ml/s @21:17:11.58 SS.seek 25ml @21:17:34.92 Vacuum too high -- waiting up to 40 seconds @21:17:34.93 SS.reconfigure :exhale @21:17:34.99 SS.hold @21:17:46.25 Intake @16.8psia, vacuum from 10.0 to 13.0psi @21:17:46.27 SS.reconfigure :inhaling 0.73ml/s @21:17:46.32 SS.seek 25ml @21:17:54.64 Stroke completed -- waiting for pressure to equalize ... @21:17:54.65 SS.reconfigure :exhale @21:17:54.70 SS.hold @21:18:15.16 Intake.select :close @21:18:15.32 SSV.dial :exhaust @21:18:16.08 Exhaust.select :open @21:18:16.20 SS.seek 0ml @21:18:25.00 Exhaust.select :close @21:18:25.11 SSV.dial CBV @21:18:25.13
CBV.dial SSV @21:18:25.91 Allowing 130s for 25.0ml of remaining 800.0ml @21:18:25.92 Intake.select :open @21:18:28.09 Intake @15.6psia, vacuum from 10.0 to 13.0psi @21:18:28.10 SS.reconfigure :inhaling 0.73ml/s @21:18:28.15 SS.seek 25ml @21:19:04.67 Stroke completed -- waiting for pressure to equalize ... @21:19:04.68 SS.reconfigure :exhale @21:19:04.73 SS.hold @21:19:33.35 Intake.select :close @21:19:33.50 SSV.dial :exhaust @21:19:34.27 Exhaust.select :open @21:19:34.39 SS.seek 0ml @21:19:43.16 Exhaust.select :close @21:19:43.27 SSV.dial CBV @21:19:43.29
CBV.dial SSV @21:19:44.05 Allowing 130s for 25.0ml of remaining 775.0ml @21:19:44.06 Intake.select :open @21:19:46.23 Intake @15.7psia, vacuum from 10.0 to 13.0psi @21:19:46.24 SS.reconfigure :inhaling 0.73ml/s @21:19:46.29 SS.seek 25ml @21:20:17.85 Vacuum too high -- waiting up to 40 seconds @21:20:17.87 SS.reconfigure :exhale @21:20:17.92 SS.hold @21:20:36.47 Intake @17.4psia, vacuum from 10.0 to 13.0psi @21:20:36.49 SS.reconfigure :inhaling 0.59ml/s @21:20:36.54 SS.seek 25ml @21:20:42.75 Stroke completed -- waiting for pressure to equalize ... @21:20:42.76 SS.reconfigure :exhale @21:20:42.81 SS.hold @21:21:01.17 Intake.select :close @21:21:01.33 SSV.dial :exhaust @21:21:02.08 Exhaust.select :open @21:21:02.63 SS.seek 0ml @21:21:11.44 Exhaust.select :close @21:21:11.55 SSV.dial CBV @21:21:11.57
CBV.dial SSV @21:21:12.36 Allowing 130s for 25.0ml of remaining 750.0ml @21:21:12.37 Intake.select :open @21:21:14.54 Intake @15.4psia, vacuum from 9.9 to 12.9psi @21:21:14.56 SS.reconfigure :inhaling 0.59ml/s @21:21:14.61 SS.seek 25ml @21:21:59.42 Stroke completed -- waiting for pressure to equalize ... @21:21:59.43 SS.reconfigure :exhale @21:21:59.48 SS.hold @21:22:36.29 Intake.select :close @21:22:36.44 SSV.dial :exhaust @21:22:37.21 Exhaust.select :open @21:22:37.32 SS.seek 0ml @21:22:46.06 Exhaust.select :close @21:22:46.17 SSV.dial CBV @21:22:46.21
CBV.dial SSV @21:22:46.98 Allowing 130s for 25.0ml of remaining 725.0ml @21:22:47.00 Intake.select :open @21:22:49.17 Intake @15.4psia, vacuum from 9.9 to 12.9psi @21:22:49.18 SS.reconfigure :inhaling 0.59ml/s @21:22:49.23 SS.seek 25ml @21:23:33.86 Stroke completed -- waiting for pressure to equalize ... @21:23:33.87 SS.reconfigure :exhale @21:23:33.91 SS.hold @21:24:14.09 SS.reconfigure :default @21:24:14.14 SS.hold @21:24:14.20 Intake.select :close @21:24:14.36 ShallowSampler::Clogged in MAIN -- Intake @16psia & Outlet @13psia won't equalize after 300.0ml @21:24:14.37 SSV.dial :exhaust @21:24:15.15 Exhaust.select :open @21:24:15.27 SS.reconfigure :exhale @21:24:15.32 SS.seek 0ml @21:24:24.15 Exhaust.select :close @21:24:24.27 Sampled 300.0ml @21:24:24.28 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @21:24:24.29 Intake.select :close @21:24:24.35 CSV.dial :waste @21:24:24.51 SSV.dial CBV,avoiding: :exhaust @21:24:24.55 CS.seek 0ml @21:24:24.59
CBV.dial SSV @21:24:24.73 CSV.dial :air @21:24:25.59 CS.seek 10ml @21:24:26.44
CTV.dial :air @21:24:27.23 delay 15 seconds @21:24:42.25 SSV.dial :spit,avoiding: :exhaust @21:24:43.57 CTV.dial CSV,avoiding: :intake @21:24:43.61
SS.seek 0ml @21:24:43.72 SSV.dial CBV,avoiding: :exhaust @21:24:50.26 SSV.dial between CBV and plug @21:24:50.75 CBV.dial between SSV and waste2 @21:24:51.15 CTV.dial CSV,avoiding: :air @21:24:51.17
CSV.dial CTV @21:24:51.71 CS.reconfigure :pressurize @21:24:51.76 CS.seek 0ml @21:25:09.89 BubblePoint: Intake @24.2psia, Outlet @0.8psia @21:25:09.90 CBV.dial SSV @21:25:10.24 SS.reconfigure :default @21:25:10.29 SS.seek 10ml @21:25:15.89 delay 15 seconds @21:25:31.02 Evacuated: Intake @23.7psia, Outlet @24.2psia @21:25:31.03 SSV.dial :spit,avoiding: :exhaust @21:25:32.07 SS.reconfigure :exhale @21:25:32.11 SS.seek 0ml @21:25:37.58 SS.reconfigure :fast @21:25:37.62 SS.seek 5ml @21:25:40.61 SSV.dial CBV,avoiding: :exhaust @21:25:40.63
CBV.dial SSV @21:25:42.00 CTV.dial :air,avoiding: :intake @21:25:43.33 CSV.dial CTV @21:25:43.39
SS.seek 10ml @21:25:44.56 CSV.dial :air @21:25:44.99 CS.reconfigure :fast @21:25:45.04 CS.seek 0ml @21:25:46.03
SSV.dial :spit,avoiding: :exhaust @21:25:47.35 SS.reconfigure :exhale @21:25:47.40 SS.seek 0ml @21:25:52.41 SS.reconfigure :fast @21:25:52.46 SS.seek 5ml @21:25:53.00 CS.stop @21:25:55.47 SSV.dial CBV,avoiding: :exhaust @21:25:55.49
CBV.dial SSV @21:25:57.87 SSV.dial :spit,avoiding: :exhaust @21:25:59.19 SS.reconfigure :default @21:25:59.24 SS.seek 0ml @21:26:02.26 SS.stop @21:26:02.30 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @21:26:02.31 Intake.select :close @21:26:02.38 CSV.dial :waste @21:26:02.51 SSV.dial CBV,avoiding: :exhaust @21:26:02.55
CBV.dial SSV @21:26:03.17 CS.seek 0ml @21:26:03.23 CSV.dial :air @21:26:03.86
CTV.dial :air @21:26:03.96 CS.seek 10ml @21:26:04.04
delay 15 seconds @21:26:19.06 SSV.dial :spit,avoiding: :exhaust @21:26:20.38 CTV.dial CSV,avoiding: :intake @21:26:20.41
SS.reconfigure :exhale @21:26:20.51 SS.seek 0ml @21:26:20.97 SSV.dial CBV,avoiding: :exhaust @21:26:28.60 SSV.dial between CBV and plug @21:26:29.09 CBV.dial between SSV and waste2 @21:26:29.50 CSV.dial between air and CTV @21:26:29.82 CS.seek 7.971ml @21:26:35.32 CTV.dial CSV,avoiding: :air @21:26:35.34
CSV.dial CTV @21:26:35.70 CS.reconfigure :pressurize @21:26:35.74 CS.seek 0ml @21:26:50.45 BubblePoint: Intake @24.1psia, Outlet @3.2psia @21:26:50.46 CBV.dial SSV @21:26:50.79 SS.reconfigure :default @21:26:50.84 SS.seek 10ml @21:26:56.79 delay 15 seconds @21:27:12.69 Evacuated: Intake @23.8psia, Outlet @24.2psia @21:27:12.71 SSV.dial :spit,avoiding: :exhaust @21:27:13.75 SS.reconfigure :exhale @21:27:13.80 SS.seek 0ml @21:27:18.76 SS.reconfigure :fast @21:27:18.80 SS.seek 5ml @21:27:21.60 SSV.dial CBV,avoiding: :exhaust @21:27:21.62
CBV.dial SSV @21:27:22.97 CTV.dial :air,avoiding: :intake @21:27:24.33 CSV.dial CTV @21:27:24.39
SS.seek 10ml @21:27:25.50 CSV.dial :air @21:27:25.96 CS.reconfigure :fast @21:27:26.01 CS.seek 0ml @21:27:26.90
SSV.dial :spit,avoiding: :exhaust @21:27:28.24 SS.reconfigure :exhale @21:27:28.28 SS.seek 0ml @21:27:32.62 CS.stop @21:27:33.51
SS.reconfigure :fast @21:27:33.55 SS.seek 5ml @21:27:36.30 SSV.dial CBV,avoiding: :exhaust @21:27:36.34
CBV.dial SSV @21:27:38.65 SSV.dial :spit,avoiding: :exhaust @21:27:39.97 SS.reconfigure :default @21:27:40.02 SS.seek 0ml @21:27:43.29 SS.stop @21:27:43.33 Lysing: Adding 2.0ml AqMeOH @21:27:43.34 CBV.dial between CSV and waste1,avoiding: :DBV @21:27:44.86 CTV.dial CSV @21:27:46.19 CSV.dial CSR @21:27:46.98 Pulling 1ml of CSR.AqMeOH @21:27:46.99 CSR.select :AqMeOH @21:27:47.10 CS.reconfigure :default @21:27:47.15 CS.seek 1ml @21:27:50.60 delay 5 seconds @21:27:55.61 CSR.select :air @21:27:55.78 CS.seek 6ml @21:28:09.47 delay 2 seconds @21:28:11.49 CSV.dial :waste2 @21:28:12.05 CS.reconfigure :fast @21:28:12.10 CS.seek 0ml @21:28:26.53 CSV.dial CSR @21:28:26.99 Pulling 2ml of CSR.AqMeOH CSR.select :AqMeOH @21:28:27.11 CS.reconfigure :default @21:28:27.16 CS.seek 2ml @21:28:33.16 delay 5 seconds @21:28:38.18 CSR.select :air @21:28:38.34 CS.seek 5ml @21:28:47.09 delay 2 seconds @21:28:49.11 CSV.dial CTV @21:28:49.56 CS.seek 1ml @21:29:00.90 Before lysing Collection Puck Heater at 12.8C/29.8psia @21:29:00.92 Heating Puck.from 6, :sh1 in Collection Clamp to 70C for 10:00 @21:29:00.94 CH.seek 70C,8:00 @21:31:12.71 CH.hold 10:00,70C @21:31:12.91 Begun lysing Collection Puck Heater at 68C/37.2psia @21:41:12.92 Ended lysing Collection Puck Heater at 70C/36.2psia @21:41:12.93 CS.seek 0ml @21:41:16.28 CTV.dial between spare and air,avoiding: :intake @21:41:17.30 CSV.dial :air @21:41:17.76 CS.seek 5ml @21:41:31.52 CSV.dial CTV @21:41:32.10 delay 2 seconds @21:41:34.12 CSV.dial :air @21:41:34.55 delay 2 seconds @21:41:36.57 CSV.dial CTV @21:41:37.02 delay 2 seconds @21:41:39.04 CSV.dial :air @21:41:39.51 delay 2 seconds @21:41:41.53 CS.seek 0ml @21:41:55.17 Puck @80.2psia after final lysis pressurization @21:41:55.18 Recovering Lysate into Collection syringe @21:41:55.19 CSV.dial CBV @21:41:56.21 CBV.dial CSV @21:41:56.58 CS.seek 5ml @21:42:10.15 delay 5 seconds @21:42:15.16 CTV.dial :air @21:42:15.69 CS.seek 7.001ml @21:42:21.86 delay 5 seconds @21:42:26.88 CSV.dial :air2 @21:42:27.32 CS.seek 10ml @21:42:35.80 CSV.dial between air and CTV,avoiding: CBV @21:42:36.98 Lysing Complete @21:42:37.05 Storing sh1 puck in Collection Clamp to tube 5 @21:42:37.22 SC.reconfigure :tight @21:42:37.27 SC.seek 5 @21:42:40.84 SC.configure :limp @21:42:41.50 Elbow.reconfigure :tight @21:42:41.54 Elbow.seek 5 @21:42:43.34 Forearm.reconfigure :tight @21:42:43.39 Forearm.seek 5 + 450 counts @21:42:44.42 SE.reconfigure :pick @21:42:44.48 SE.seek :beamBreak @21:42:46.54 SE.configure :default @21:42:46.70 Elbow.reconfigure :limp @21:42:46.75 Forearm.reconfigure :loose @21:42:46.80 Forearm.seek 5 @21:42:47.06 Elbow.hold @21:42:47.13 Elbow.reconfigure :tight @21:42:47.23 SE.seek 15.15 Pucks below Top Plate @21:42:47.77 Forearm.force -20 @21:42:47.81 Forearm.seek 5 + 314 counts @21:42:48.12 Forearm.reconfigure :tight @21:42:48.17 Forearm.seek 5 + 450 counts @21:42:48.38 SE.seek 16.15 Pucks below Top Plate @21:42:48.96 Forearm.reconfigure :loose @21:42:49.00 Forearm.seek :retract @21:42:49.94 Elbow.seek CC @21:42:51.64 Elbow.reconfigure :loose @21:42:51.74 Forearm.seek CC @21:42:52.67 Hand.close @21:42:53.27 CC.seek :open @21:42:58.00 Forearm.seek :retract @21:42:58.95 Elbow.reconfigure :tight @21:42:59.00 Elbow.seek 5 @21:43:00.75 Elbow.reconfigure :limp @21:43:01.24 Forearm.seek 5 @21:43:02.31 Elbow.hold @21:43:02.38 Elbow.reconfigure :tight @21:43:02.43 Hand.open @21:43:03.13 Forearm.force -20 @21:43:03.17 Forearm.seek 5 + 307 counts @21:43:03.48 Forearm.reconfigure :tight @21:43:03.53 Forearm.seek 5 + 450 counts @21:43:03.78 SE.seek :down @21:43:04.92
Forearm.reconfigure :loose @21:43:04.96 Forearm.seek :retract @21:43:05.38 SE.stop @21:43:05.92
Tranferring DA @21:43:05.93 Beginning samplePassC @21:43:05.95 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @21:43:06.14 Elbow.seek FlushPuck::Garage @21:43:07.90 Elbow.reconfigure :loose @21:43:08.00 Forearm.seek :garage @21:43:08.97 Hand.close @21:43:09.76 Forearm.seek :retract @21:43:10.78 Elbow.reconfigure :tight @21:43:10.83 Elbow.seek CC @21:43:13.78 Elbow.reconfigure :loose @21:43:13.88 Forearm.reconfigure :tight @21:43:13.93 Forearm.seek CC @21:43:15.18 CC.seek :closed @21:43:19.71 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.41 @21:43:19.78 Hand.open @21:43:20.46 Forearm.reconfigure :loose @21:43:20.50 Forearm.seek :retract @21:43:21.43 PSV.dial PSR @21:43:22.82 Pulling 1.5ml of PSR.flush @21:43:22.83 PSR.select :flush @21:43:22.95 PS.seek 1.5ml @21:43:39.06 delay 5 seconds @21:43:44.08 PSV.dial :waste1 @21:43:44.57 PS.seek 0ml @21:44:00.65 PSV.dial PSR @21:44:01.12 PSR.select :air @21:44:01.24 PS.seek 2.5ml @21:44:27.57 PRV.dial CBV,avoiding: :ab2 @21:44:27.58
CBV.dial PRV @21:44:29.36 PSV.dial :mixloop @21:44:30.41 CTV.dial :air,avoiding: :intake @21:44:30.58 PTV.dial :PRVmixing @21:44:31.91 PS.seek 0ml @21:44:58.34 PSV.dial :air @21:44:58.81 PS.seek 2.5ml @21:45:25.09 PSV.dial :mixloop @21:45:25.56 PS.seek 0ml @21:45:51.93 PSV.dial :air @21:45:52.41 PS.seek 2.5ml @21:46:18.93 PSV.dial :mixloop @21:46:19.41 PS.seek 0ml @21:46:45.75 Garaging FlushPuck in Collection Clamp @21:46:45.82 Elbow.reconfigure :tight @21:46:45.86 Elbow.seek CC @21:46:46.16 Elbow.reconfigure :loose @21:46:46.26 Forearm.seek CC @21:46:47.31 Hand.close @21:46:48.29 CC.seek :open @21:46:52.98 Forearm.seek :retract @21:46:54.00 Elbow.reconfigure :tight @21:46:54.05 Elbow.seek :garage @21:46:56.93 Elbow.reconfigure :loose @21:46:57.03 Forearm.reconfigure :tight @21:46:57.08 Forearm.seek :garage @21:46:58.11 Hand.open @21:46:58.82 Forearm.reconfigure :loose @21:46:58.87 Forearm.seek :retract @21:46:59.84 PSV.dial :mixloop @21:46:59.91 PTV.dial :PRVmixing @21:47:00.00 PRV.dial CBV @21:47:00.02
CBV.dial PRV @21:47:00.22 CTV.dial :air,avoiding: :intake @21:47:00.24 Loading lyfil puck from tube 6 into Collection Clamp @21:47:00.47 SC.reconfigure :tight @21:47:00.51 SC.seek 6 @21:47:04.19 SC.configure :limp @21:47:04.41 Elbow.reconfigure :tight @21:47:04.45 Elbow.seek 6 @21:47:06.38 Forearm.reconfigure :tight @21:47:06.43 Forearm.seek 6 + 450 counts @21:47:07.46 SE.reconfigure :pick @21:47:07.52 SE.seek :beamBreak @21:47:09.68 SE.configure :default @21:47:09.84 Elbow.seek 6 @21:47:10.02 Forearm.seek 6 + 300 counts @21:47:10.24 Forearm.reconfigure :loose @21:47:10.66 Hand.close @21:47:11.40 SE.seek :down @21:47:11.60
Forearm.seek :retract @21:47:12.54 Elbow.seek CC @21:47:13.13 SE.stop @21:47:14.18
Elbow.reconfigure :loose @21:47:14.28 Forearm.reconfigure :tight @21:47:14.33 Forearm.seek CC @21:47:15.55 CC.seek :closed @21:47:20.08 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.17 @21:47:20.09 Hand.open @21:47:20.83 Forearm.reconfigure :loose @21:47:20.88 Forearm.seek :retract @21:47:21.82 CTV.dial CSV,avoiding: :intake @21:47:23.17 CSV.dial CTV @21:47:23.51 PS.seek 2.5ml @21:47:49.99 CS.seek 4.5ml @21:48:04.89 delay 8 seconds @21:48:12.91 PSV.dial :air @21:48:13.37 delay 2 seconds @21:48:15.39 PSV.dial :waste2 @21:48:16.12 PS.seek 0ml @21:48:42.65 CBV.dial between PRV and waste1 @21:48:42.97 CSV.dial :air @21:48:43.44 CS.seek 10ml @21:48:58.29 CSV.dial CTV @21:48:58.75 CS.seek 0ml @21:49:25.14 CBV.dial :waste1 @21:49:25.90 delay 5 seconds @21:49:30.92 CTV.dial :air,avoiding: :intake @21:49:32.32 Storing lyfil puck in Collection Clamp to tube 5 @21:49:32.50 SC.reconfigure :tight @21:49:32.55 SC.seek 5 @21:49:36.15 SC.configure :limp @21:49:36.38 Elbow.reconfigure :tight @21:49:36.42 Elbow.seek 5 @21:49:38.21 Forearm.reconfigure :tight @21:49:38.26 Forearm.seek 5 + 450 counts @21:49:39.27 SE.reconfigure :pick @21:49:39.33 SE.seek :beamBreak @21:49:41.40 SE.configure :default @21:49:41.56 Elbow.reconfigure :limp @21:49:41.62 Forearm.reconfigure :loose @21:49:41.66 Forearm.seek 5 @21:49:41.91 Elbow.hold @21:49:41.98 Elbow.reconfigure :tight @21:49:42.08 SE.seek 16.15 Pucks below Top Plate @21:49:43.15 Forearm.force -20 @21:49:43.19 Forearm.seek 5 + 314 counts @21:49:43.52 Forearm.reconfigure :tight @21:49:43.57 Forearm.seek 5 + 450 counts @21:49:43.76 SE.seek 17.15 Pucks below Top Plate @21:49:44.40 Forearm.reconfigure :loose @21:49:44.45 Forearm.seek :retract @21:49:45.39 Elbow.seek CC @21:49:47.05 Elbow.reconfigure :loose @21:49:47.58 Forearm.seek CC @21:49:48.57 Hand.close @21:49:49.14 CC.seek :open @21:49:53.87 Forearm.seek :retract @21:49:54.86 Elbow.reconfigure :tight @21:49:54.90 Elbow.seek 5 @21:49:56.72 Elbow.reconfigure :limp @21:49:56.77 Forearm.seek 5 @21:49:57.84 Elbow.hold @21:49:57.90 Elbow.reconfigure :tight @21:49:57.95 Hand.open @21:49:58.63 Forearm.force -20 @21:49:58.68 Forearm.seek 5 + 315 counts @21:49:58.97 Forearm.reconfigure :tight @21:49:59.02 Forearm.seek 5 + 450 counts @21:49:59.28 SE.seek :down @21:50:00.41
Forearm.reconfigure :loose @21:50:00.46 Forearm.seek :retract @21:50:00.81 SE.stop @21:50:01.40
PSV.dial :air @21:50:02.15 PS.seek 2.5ml @21:50:28.52 delay 1 second @21:50:29.54 PSV.dial :mixloop @21:50:29.99 delay 1 second @21:50:31.01 PSV.dial :air @21:50:31.47 delay 1 second @21:50:32.49 PSV.dial :mixloop @21:50:32.96 delay 1 second @21:50:33.97 PSV.dial :air @21:50:34.44 delay 1 second @21:50:35.47 PS.seek 0ml @21:51:01.87 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @21:51:02.48 Elbow.seek FlushPuck::Garage @21:51:04.22 Elbow.reconfigure :loose @21:51:04.33 Forearm.seek :garage @21:51:05.35 Hand.close @21:51:06.13 Forearm.seek :retract @21:51:07.15 Elbow.reconfigure :tight @21:51:07.19 Elbow.seek PC @21:51:08.47 Elbow.reconfigure :loose @21:51:08.57 Forearm.reconfigure :tight @21:51:08.62 Forearm.seek PC @21:51:09.88 PC.seek :closed @21:51:14.50 Processing Clamp onSpring/offSpring for 16/26 samples, on/off torque 1.65 @21:51:14.56 Hand.open @21:51:15.19 Forearm.reconfigure :loose @21:51:15.24 Forearm.seek :retract @21:51:16.26 PRV.dial :air @21:51:16.71 PSV.dial :mixloop @21:51:17.25 PS.seek 0.3ml @21:51:21.11 PSV.dial PSR @21:51:22.16 Pulling 0.4ml of PSR.air @21:51:22.17 PSR.select :air @21:51:22.19 PS.seek 0.7ml @21:51:27.11 delay 3 seconds @21:51:30.13 PSR.select :air @21:51:30.15 PS.seek 2.5ml @21:51:49.36 delay 3 seconds @21:51:52.38 PSV.dial :mixloop @21:51:53.44 PTV.dial :mixing @21:51:54.26 PBV.dial :waste2 @21:51:55.23 PS.seek 0ml @21:52:21.52 Garaging FlushPuck in Processing Clamp @21:52:21.59 Elbow.reconfigure :tight @21:52:21.64 Elbow.seek PC @21:52:21.76 Elbow.reconfigure :loose @21:52:21.86 Forearm.seek PC @21:52:22.87 Hand.close @21:52:23.51 PC.seek :open @21:52:28.23 Forearm.seek :retract @21:52:29.20 Elbow.reconfigure :tight @21:52:29.24 Elbow.seek :garage @21:52:30.67 Elbow.reconfigure :loose @21:52:30.77 Forearm.reconfigure :tight @21:52:30.82 Forearm.seek :garage @21:52:31.86 Hand.open @21:52:32.57 Forearm.reconfigure :loose @21:52:32.62 Forearm.seek :retract @21:52:33.66 Loading da puck from tube 6 into Processing Clamp @21:52:33.89 SC.reconfigure :tight @21:52:33.94 SC.seek 6 @21:52:37.48 SC.configure :limp @21:52:37.70 Elbow.reconfigure :tight @21:52:37.74 Elbow.seek 6 @21:52:39.66 Forearm.reconfigure :tight @21:52:39.70 Forearm.seek 6 + 450 counts @21:52:40.82 SE.reconfigure :pick @21:52:40.87 SE.seek :beamBreak @21:52:43.06 SE.configure :default @21:52:43.22 Elbow.seek 6 @21:52:43.85 Forearm.seek 6 + 300 counts @21:52:44.02 Forearm.reconfigure :loose @21:52:44.07 Hand.close @21:52:44.66 SE.seek :down @21:52:44.85
Forearm.seek :retract @21:52:45.82 Elbow.seek PC @21:52:46.53 SE.stop @21:52:48.58
Elbow.reconfigure :loose @21:52:48.68 Forearm.reconfigure :tight @21:52:48.73 Forearm.seek PC @21:52:49.98 PC.seek :closed @21:52:54.62 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.67 @21:52:54.63 Hand.open @21:52:55.31 Forearm.reconfigure :loose @21:52:55.36 Forearm.seek :retract @21:52:56.37 Heating Puck.from 6, :da in Processing Clamp to 28C for 2:00:00 @21:52:56.39 PH.seek 28C,5:00 @21:53:37.01 PH.hold 2:00:00,28C @21:53:37.06 delay 1:30 @21:55:07.10 PSR.select :air @21:55:07.12 PSV.dial PSR @21:55:08.17 Pulling 0.5ml of PSR.wash @21:55:08.18 PSR.select :wash @21:55:08.31 PS.seek 0.5ml @21:55:14.20 delay 5 seconds @21:55:19.22 PSR.select :air @21:55:19.35 PS.seek 2ml @21:55:35.45 PTV.dial :puck @21:55:36.20 PSV.dial PTV @21:55:36.97 PBV.dial :waste1 @21:55:38.36 PS.seek 1.5ml @21:55:44.30 delay 3 seconds @21:55:47.31 PBV.dial between PSV and waste1 @21:55:47.68 PS.seek 0ml @21:56:03.86 delay 5:00 @22:01:03.89 recoverPSRreagent08 PTV.dial :puck @22:01:03.96 PBV.dial :waste1 @22:01:04.34 delay 30 seconds @22:01:34.36 PBV.dial between PSV and waste1 @22:01:34.74 PSR.select :air @22:01:34.76 PSV.dial PSR @22:01:35.52 PS.reconfigure :fast @22:01:35.56 PS.seek 2.5ml @22:01:59.29 PSV.dial PTV @22:02:00.06 delay 5 seconds @22:02:05.08 PSV.dial PSR @22:02:05.84 delay 5 seconds @22:02:10.86 PSV.dial PTV @22:02:11.63 delay 5 seconds @22:02:16.65 PSV.dial :air @22:02:17.44 PS.seek 0ml @22:02:41.04 PTV.dial :PRVmixing,via: PRV @22:02:42.37 PSV.dial :mixloop @22:02:42.84 PRV.dial :ab1 @22:02:43.59 PS.reconfigure :slow @22:02:43.64 PS.seek 0.15ml @22:02:47.20 delay 2 seconds @22:02:49.22 CBV.dial PRV @22:02:49.25
PRV.dial CBV @22:02:49.81 PS.seek 0.35ml @22:02:54.45 delay 2 seconds @22:02:56.46 CBV.dial between PRV and PSV @22:02:56.80 PRV.dial :air @22:02:57.25 PS.seek 0.9ml @22:03:09.07 PRV.dial :waste2 @22:03:09.83 PS.seek 0.5ml @22:03:18.45 addPMLReagent08 @22:03:18.46 PSV.dial :air @22:03:18.92 PS.reconfigure :default @22:03:18.97 PS.seek 2ml @22:03:35.16 PSV.dial :PML @22:03:35.63 PTV.dial :mixing @22:03:36.38 PBV.dial :waste1 @22:03:36.82 PS.seek 1.85ml @22:03:39.16 PBV.dial between PSV and waste1 @22:03:39.54 PS.seek 0ml @22:03:59.19 delay 2 seconds @22:04:01.21 Beginning samplePassC @22:04:01.23 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @22:04:01.41 Elbow.reconfigure :tight @22:04:01.46 Elbow.seek FlushPuck::Garage @22:04:02.77 Elbow.reconfigure :loose @22:04:02.87 Forearm.seek :garage @22:04:03.89 Hand.close @22:04:04.62 Forearm.seek :retract @22:04:05.61 Elbow.reconfigure :tight @22:04:05.66 Elbow.seek CC @22:04:08.57 Elbow.reconfigure :loose @22:04:08.67 Forearm.reconfigure :tight @22:04:09.15 Forearm.seek CC @22:04:10.39 CC.seek :closed @22:04:14.92 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.48 @22:04:14.98 Hand.open @22:04:15.69 Forearm.reconfigure :loose @22:04:15.74 Forearm.seek :retract @22:04:16.75 PSV.dial PSR @22:04:17.80 Pulling 1.5ml of PSR.flush @22:04:17.81 PSR.select :flush @22:04:17.93 PS.seek 1.5ml @22:04:34.10 delay 5 seconds @22:04:39.12 PSV.dial :waste1 @22:04:39.59 PS.seek 0ml @22:04:55.78 PSV.dial PSR @22:04:56.25 PSR.select :air @22:04:56.37 PS.seek 2.5ml @22:05:22.67 PRV.dial CBV,avoiding: :ab2 @22:05:22.69
CBV.dial PRV @22:05:24.30 PSV.dial :mixloop @22:05:25.35 CTV.dial :air,avoiding: :intake @22:05:25.42 PTV.dial :PRVmixing @22:05:26.18 PS.seek 0ml @22:05:52.60 PSV.dial :air @22:05:53.07 PS.seek 2.5ml @22:06:19.44 PSV.dial :mixloop @22:06:19.92 PS.seek 0ml @22:06:46.28 PSV.dial :air @22:06:46.76 PS.seek 2.5ml @22:07:13.06 PSV.dial :mixloop @22:07:13.54 PS.seek 0ml @22:07:39.91 Garaging FlushPuck in Collection Clamp @22:07:40.43 Elbow.reconfigure :tight @22:07:40.48 Elbow.seek CC @22:07:40.79 Elbow.reconfigure :loose @22:07:40.89 Forearm.seek CC @22:07:41.95 Hand.close @22:07:42.62 CC.seek :open @22:07:47.27 Forearm.seek :retract @22:07:48.25 Elbow.reconfigure :tight @22:07:48.29 Elbow.seek :garage @22:07:51.30 Elbow.reconfigure :loose @22:07:51.40 Forearm.reconfigure :tight @22:07:51.45 Forearm.seek :garage @22:07:52.47 Hand.open @22:07:53.18 Forearm.reconfigure :loose @22:07:53.23 Forearm.seek :retract @22:07:54.22 delayUntil "22:12:01.20" @22:07:54.25 Whole Cell Archive sampling 1000ml @22:07:54.26 Loading wcr puck from tube 6 into Collection Clamp @22:07:54.74 Elbow.reconfigure :tight @22:07:54.79 Elbow.seek 6 @22:07:56.68 Forearm.reconfigure :tight @22:07:56.73 Forearm.seek 6 + 450 counts @22:07:57.99 SE.reconfigure :pick @22:07:58.05 SE.seek :beamBreak @22:08:00.35 SE.configure :default @22:08:00.52 Elbow.seek 6 @22:08:01.15 Forearm.seek 6 + 300 counts @22:08:01.32 Forearm.reconfigure :loose @22:08:01.37 Hand.close @22:08:02.01 SE.seek :down @22:08:02.22 Forearm.seek :retract @22:08:03.18 Elbow.seek CC @22:08:03.94 SE.stop @22:08:04.82 Elbow.reconfigure :loose @22:08:04.92 Forearm.reconfigure :tight @22:08:04.98 Forearm.seek CC @22:08:06.18 CC.seek :closed @22:08:10.71 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.41 @22:08:10.72 Hand.open @22:08:11.43 Forearm.reconfigure :loose @22:08:11.48 Forearm.seek :retract @22:08:12.42 collectSample 1000ml w/bubble point of 35psi @22:08:12.43 Priming 7ml @22:08:12.44 Intake.select :close @22:08:12.45 Exhaust.select :close @22:08:12.47 SS.hold @22:08:12.68 Calibrating pressure sensors @22:08:12.69 CTV.dial :air @22:08:12.70 SSV.dial :spit,avoiding: :exhaust @22:08:12.78 SS.reconfigure :fast @22:08:12.84 SS.seek 5ml @22:08:15.56 SSV.dial CBV @22:08:15.58 CBV.dial SSV @22:08:16.95 delay 3.8 seconds @22:08:21.35 Sensor limits: Intake max 101.7psia; Outlet max 93.5psia @22:08:21.36 SSV.dial :spit @22:08:22.71 SS.seek 0ml @22:08:27.04 SSV.dial CBV @22:08:28.37 CTV.dial :intake @22:08:29.14 SSV.dial CBV @22:08:29.16 CBV.dial SSV @22:08:29.31 Allowing 40s for 7.0ml of remaining 7.0ml @22:08:29.32 Intake.select :open @22:08:31.50 Intake @14.4psia, vacuum from 8.9 to 11.9psi @22:08:31.52 SS.reconfigure :inhaling 2.20ml/s @22:08:31.57 SS.seek 7ml @22:08:38.55 Stroke completed -- waiting for pressure to equalize ... @22:08:38.57 SS.reconfigure :exhale @22:08:38.62 SS.hold @22:09:03.58 Intake.select :close @22:09:03.74 SSV.dial :exhaust @22:09:04.53 Exhaust.select :open @22:09:04.64 SS.seek 0ml @22:09:08.93 Exhaust.select :close @22:09:09.04 Sampling 1000.0ml @22:09:09.06 Intake.select :close @22:09:09.07 Exhaust.select :close @22:09:09.08 SS.reconfigure :default @22:09:09.12 SS.hold @22:09:09.33 CTV.dial :intake @22:09:09.51 SSV.dial CBV @22:09:09.55 CBV.dial SSV @22:09:10.34 Allowing 130s for 25.0ml of remaining 1000.0ml @22:09:10.35 Intake.select :open @22:09:12.52 Intake @15.3psia, vacuum from 9.8 to 12.8psi @22:09:12.54 SS.reconfigure :inhaling 2.20ml/s @22:09:12.59 SS.seek 25ml @22:09:19.74 Vacuum too high -- waiting up to 40 seconds @22:09:19.75 SS.reconfigure :exhale @22:09:19.81 SS.hold @22:09:30.05 Intake @18.7psia, vacuum from 10.0 to 13.0psi @22:09:30.06 SS.reconfigure :inhaling 1.76ml/s @22:09:30.11 SS.seek 25ml @22:09:32.20 Vacuum too high -- waiting up to 40 seconds @22:09:32.22 SS.reconfigure :exhale @22:09:32.28 SS.hold @22:09:32.84 Intake @15.8psia, vacuum from 10.0 to 13.0psi @22:09:32.86 SS.reconfigure :inhaling 1.41ml/s @22:09:32.91 SS.seek 25ml @22:09:37.73 Vacuum too high -- waiting up to 40 seconds @22:09:37.75 SS.reconfigure :exhale @22:09:37.80 SS.hold @22:09:45.91 Intake @19.0psia, vacuum from 10.0 to 13.0psi @22:09:45.93 SS.reconfigure :inhaling 1.13ml/s @22:09:45.98 SS.seek 25ml @22:09:49.08 Vacuum too high -- waiting up to 40 seconds @22:09:49.10 SS.reconfigure :exhale @22:09:49.15 SS.hold @22:09:50.87 Intake @17.7psia, vacuum from 10.0 to 13.0psi @22:09:50.89 SS.reconfigure :inhaling 0.91ml/s @22:09:50.93 SS.seek 25ml @22:09:54.68 Vacuum too high -- waiting up to 40 seconds @22:09:54.70 SS.reconfigure :exhale @22:09:54.75 SS.hold @22:09:57.84 Intake @18.4psia, vacuum from 10.0 to 13.0psi @22:09:57.86 SS.reconfigure :inhaling 0.73ml/s @22:09:57.90 SS.seek 25ml @22:10:00.60 Stroke completed -- waiting for pressure to equalize ... @22:10:00.61 SS.reconfigure :exhale @22:10:00.66 SS.hold @22:10:31.82 Intake.select :close @22:10:31.99 SSV.dial :exhaust @22:10:32.76 Exhaust.select :open @22:10:32.88 SS.seek 0ml @22:10:41.74 Exhaust.select :close @22:10:41.86 SSV.dial CBV @22:10:41.88 CBV.dial SSV @22:10:43.09 Allowing 130s for 25.0ml of remaining 975.0ml @22:10:43.10 Intake.select :open @22:10:45.27 Intake @15.8psia, vacuum from 10.0 to 13.0psi @22:10:45.29 SS.reconfigure :inhaling 0.73ml/s @22:10:45.34 SS.seek 25ml @22:11:22.10 Stroke completed -- waiting for pressure to equalize ... @22:11:22.11 SS.reconfigure :exhale @22:11:22.16 SS.hold @22:12:01.21 recoverPRVreagent08 @22:12:01.22 PTV.dial :mixing @22:12:01.99 PBV.dial :waste1 @22:12:02.35 SS.reconfigure :default @22:12:02.42 SS.hold @22:12:02.44 delay 30 seconds @22:12:02.52 Intake.select :close @22:12:02.68 ShallowSampler::Clogged in WCR -- Intake @16psia & Outlet @14psia won't equalize after 50.0ml @22:12:02.69 SSV.dial :exhaust @22:12:03.46 Exhaust.select :open @22:12:03.58 SS.reconfigure :exhale @22:12:03.63 SS.seek 0ml @22:12:12.66 Exhaust.select :close @22:12:12.78 Sampled 50.0ml @22:12:12.79 Drying puck before fixing for 5:00 @22:12:12.80 CBV.dial :waste2b @22:12:13.57 delay 1:00 @22:12:32.48 PBV.dial between PSV and waste1 @22:12:32.86 PSV.dial :air @22:12:33.33 PS.reconfigure :fast @22:12:33.38 PS.seek 2.5ml @22:12:57.24 PSV.dial :mixloop @22:12:57.72 delay 2 seconds @22:12:59.73 PSV.dial :air @22:13:00.20 delay 2 seconds @22:13:02.22 PSV.dial :mixloop @22:13:02.68 delay 2 seconds @22:13:04.70 PSV.dial :air @22:13:05.30 PS.seek 0ml @22:13:13.59 CSV.dial :air @22:13:14.06 CS.reconfigure :fast @22:13:14.11 CS.seek 10ml @22:13:28.56 washCycleDA #1 of 2 @22:13:28.57 PSR.select :air @22:13:28.59 PSV.dial PSR @22:13:29.93 Pulling 0.5ml of PSR.wash @22:13:29.94 PSR.select :wash @22:13:30.06 PS.reconfigure :default @22:13:30.11 PS.seek 0.5ml @22:13:36.03 delay 5 seconds @22:13:39.12 Pumping wcr puck from tube 6 up to 35psi... @22:13:39.14 CBV.dial SSV @22:13:40.42 CBV.dial between CSV and waste2b @22:13:41.04 PSR.select :air @22:13:41.16 PS.seek 2ml @22:13:41.40 CSV.dial between air and CTV @22:13:41.72 CS.seek 7.218ml @22:13:48.87 CTV.dial CSV,avoiding: :air @22:13:48.90 CSV.dial CTV @22:13:49.75 CS.reconfigure :pressurize @22:13:49.80 CS.seek 0ml @22:13:57.40 PTV.dial :puck @22:13:58.15 PSV.dial PTV @22:13:58.92 PBV.dial :waste1 @22:13:59.35 PS.seek 1.5ml @22:14:05.31 delay 3 seconds @22:14:08.33 PBV.dial between PSV and waste1 @22:14:08.42 CBV.dial :waste2b @22:14:08.70 PS.seek 0ml @22:14:08.78 CS.reconfigure :default @22:14:08.83 CS.seek 0ml @22:14:09.80 delay 1:00 @22:14:24.87 delay 2:00 @22:15:09.83 CTV.dial :air,avoiding: :intake @22:15:11.17 Fix #1 of 2 (with RNAlater) for 2:30 @22:15:11.18 CSV.dial CSR @22:15:11.76 Pulling 2ml of CSR.RNAlater @22:15:11.77 CSR.select :RNAlater @22:15:11.89 CS.seek 2ml @22:15:17.83 delay 5 seconds @22:15:22.86 CSR.select :air @22:15:22.97 CS.reconfigure :fast @22:15:23.02 CS.seek 10ml @22:15:42.74 Pumping wcr puck from tube 6 up to 35psi... @22:15:42.76 CBV.dial SSV @22:15:44.03 CBV.dial between CSV and waste2b @22:15:45.00 CSV.dial between air and CTV @22:15:45.59 CS.seek 7.234ml @22:15:52.68 CTV.dial CSV,avoiding: :air @22:15:52.70 CSV.dial CTV @22:15:54.57 CS.reconfigure :pressurize @22:15:54.63 CS.seek 0ml @22:16:08.76 delay 2:30 @22:16:24.90 recoverPSRreagent08 @22:16:24.91 PTV.dial :puck @22:16:24.98 PBV.dial :waste1 @22:16:25.35 delay 30 seconds @22:16:55.38 PBV.dial between PSV and waste1 @22:16:55.76 PSR.select :air @22:16:55.78 PSV.dial PSR @22:16:56.54 PS.reconfigure :fast @22:16:56.59 PS.seek 2.5ml @22:17:20.43 PSV.dial PTV @22:17:21.32 delay 5 seconds @22:17:26.34 PSV.dial PSR @22:17:27.09 delay 5 seconds @22:17:32.11 PSV.dial PTV @22:17:32.87 delay 5 seconds @22:17:37.89 PSV.dial :air @22:17:38.65 PS.seek 0ml @22:18:01.96 washCycleDA #2 of 2 @22:18:01.97 PSR.select :air @22:18:01.99 PSV.dial PSR @22:18:03.33 Pulling 0.5ml of PSR.wash @22:18:03.35 PSR.select :wash @22:18:03.47 PS.reconfigure :default @22:18:03.52 PS.seek 0.5ml @22:18:09.49 delay 5 seconds @22:18:14.51 PSR.select :air @22:18:14.63 PS.seek 2ml @22:18:30.80 PTV.dial :puck @22:18:30.88 PSV.dial PTV @22:18:31.63 PBV.dial :waste1 @22:18:32.06 PS.seek 1.5ml @22:18:38.05 delay 3 seconds @22:18:38.78 CBV.dial :waste2b @22:18:39.13 CS.reconfigure :default @22:18:39.18 CS.seek 0ml @22:18:41.07 PBV.dial between PSV and waste1 @22:18:41.45 PS.seek 0ml @22:18:44.74 delay 1:00 @22:18:57.58 delay 2:00 @22:19:44.76 CTV.dial :air,avoiding: :intake @22:19:46.10 Fix #2 of 2 (with RNAlater) for 2:30 @22:19:46.12 CSV.dial CSR @22:19:46.55 Pulling 2ml of CSR.RNAlater @22:19:46.56 CSR.select :RNAlater @22:19:46.68 CS.seek 2ml @22:19:52.67 delay 5 seconds @22:19:57.69 CSR.select :air @22:19:57.81 CS.reconfigure :fast @22:19:57.86 CS.seek 10ml @22:20:17.68 Pumping wcr puck from tube 6 up to 35psi... @22:20:17.69 CBV.dial SSV @22:20:18.97 CBV.dial between CSV and waste2b @22:20:19.92 CSV.dial between air and CTV @22:20:20.52 CS.seek 7.284ml @22:20:27.52 CTV.dial CSV,avoiding: :air @22:20:27.55 CSV.dial CTV @22:20:28.93 CS.reconfigure :pressurize @22:20:28.98 CS.seek 0ml @22:20:47.57 delay 2:30 @22:20:57.60 recoverPSRreagent08 @22:20:57.61 PTV.dial :puck @22:20:57.69 PBV.dial :waste1 @22:20:58.07 delay 30 seconds @22:21:28.09 PBV.dial between PSV and waste1 @22:21:28.47 PSR.select :air @22:21:28.49 PSV.dial PSR @22:21:29.26 PS.reconfigure :fast @22:21:29.31 PS.seek 2.5ml @22:21:53.02 PSV.dial PTV @22:21:53.78 delay 5 seconds @22:21:58.81 PSV.dial PSR @22:21:59.57 delay 5 seconds @22:22:04.59 PSV.dial PTV @22:22:05.35 delay 5 seconds @22:22:10.37 PSV.dial :air @22:22:11.13 PS.seek 0ml @22:22:34.49 getPMLReagent08 @22:22:34.50 PRV.dial :air @22:22:34.97 delay 5 seconds @22:22:39.99 PTV.dial :PRVmixing @22:22:41.33 delay 5 seconds @22:22:46.35 PRV.dial :ab2 @22:22:46.81 PTV.dial :PRVmixing @22:22:47.00 PSV.dial :mixloop @22:22:47.50 PS.reconfigure :default @22:22:47.55 PS.seek 0.3ml @22:22:51.43 delay 2 seconds @22:22:53.45 PRV.dial :air @22:22:53.97 PS.seek 0.55ml @22:22:57.36 addPMLReagent08 @22:22:57.37 PSV.dial :air @22:22:57.85 PS.seek 2ml @22:23:13.51 PSV.dial :PML @22:23:13.98 PTV.dial :mixing @22:23:14.75 PBV.dial :waste1 @22:23:15.18 PS.seek 1.85ml @22:23:17.51 PBV.dial between PSV and waste1 @22:23:17.59 CBV.dial :waste2b @22:23:17.90 PS.seek 0ml @22:23:17.97 CS.reconfigure :default @22:23:18.48 CS.seek 0ml @22:23:19.70 delay 1:00 @22:23:37.67 delay 2 seconds @22:23:39.69 delay 8:00 @22:24:19.73 CTV.dial :air,avoiding: :intake @22:31:39.72 recoverPRVreagent08 @22:31:39.73 PTV.dial :mixing @22:31:39.80 PBV.dial :waste1 @22:31:40.18 delay 30 seconds @22:32:10.21 PBV.dial between PSV and waste1 @22:32:10.58 PSV.dial :air @22:32:11.06 PS.reconfigure :fast @22:32:11.11 PS.seek 2.5ml @22:32:34.97 PSV.dial :mixloop @22:32:35.44 delay 2 seconds @22:32:37.46 PSV.dial :air @22:32:37.93 delay 2 seconds @22:32:39.95 PSV.dial :mixloop @22:32:40.41 delay 2 seconds @22:32:42.43 PSV.dial :air @22:32:42.90 PS.seek 0ml @22:33:06.13 washCycleDA #1 of 3 @22:33:06.14 PSR.select :air @22:33:06.16 PSV.dial PSR @22:33:07.51 Pulling 0.5ml of PSR.wash @22:33:07.52 PSR.select :wash @22:33:07.64 PS.reconfigure :default @22:33:07.68 PS.seek 0.5ml @22:33:13.63 delay 5 seconds @22:33:18.65 PSR.select :air @22:33:18.77 PS.seek 2ml @22:33:34.91 PTV.dial :puck @22:33:35.66 PSV.dial PTV @22:33:36.41 PBV.dial :waste1 @22:33:36.85 PS.seek 1.5ml @22:33:42.75 delay 3 seconds @22:33:45.77 PBV.dial between PSV and waste1 @22:33:46.15 PS.seek 0ml @22:34:02.32 delay 2:00 @22:36:02.35 recoverPSRreagent08 @22:36:02.36 PTV.dial :puck @22:36:02.42 PBV.dial :waste1 @22:36:02.80 delay 30 seconds @22:36:32.83 PBV.dial between PSV and waste1 @22:36:33.20 PSR.select :air @22:36:33.22 PSV.dial PSR @22:36:33.99 PS.reconfigure :fast @22:36:34.04 PS.seek 2.5ml @22:36:57.97 PSV.dial PTV @22:36:58.74 delay 5 seconds @22:37:03.76 PSV.dial PSR @22:37:04.53 delay 5 seconds @22:37:09.55 PSV.dial PTV @22:37:10.31 delay 5 seconds @22:37:15.34 PSV.dial :air @22:37:16.10 PS.seek 0ml @22:37:39.25 washCycleDA #2 of 3 @22:37:39.26 PSR.select :air @22:37:39.28 PSV.dial PSR @22:37:40.63 Pulling 0.5ml of PSR.wash @22:37:40.64 PSR.select :wash @22:37:40.75 PS.reconfigure :default @22:37:40.80 PS.seek 0.5ml @22:37:46.72 delay 5 seconds @22:37:52.25 PSR.select :air @22:37:52.37 PS.seek 2ml @22:38:08.56 PTV.dial :puck @22:38:08.58 PSV.dial PTV @22:38:09.33 PBV.dial :waste1 @22:38:09.76 PS.seek 1.5ml @22:38:15.66 delay 3 seconds @22:38:18.68 PBV.dial between PSV and waste1 @22:38:19.04 PS.seek 0ml @22:38:35.16 delay 2:00 @22:40:35.18 recoverPSRreagent08 @22:40:35.19 PTV.dial :puck @22:40:35.21 PBV.dial :waste1 @22:40:35.56 delay 30 seconds @22:41:05.59 PBV.dial between PSV and waste1 @22:41:05.95 PSR.select :air @22:41:05.97 PSV.dial PSR @22:41:06.74 PS.reconfigure :fast @22:41:06.79 PS.seek 2.5ml @22:41:30.65 PSV.dial PTV @22:41:31.42 delay 5 seconds @22:41:36.44 PSV.dial PSR @22:41:37.18 delay 5 seconds @22:41:42.21 PSV.dial PTV @22:41:42.97 delay 5 seconds @22:41:47.99 PSV.dial :air @22:41:48.75 PS.seek 0ml @22:42:11.97 washCycleDA #3 of 3 @22:42:11.98 PSR.select :air @22:42:11.99 PSV.dial PSR @22:42:13.32 Pulling 0.5ml of PSR.wash @22:42:13.33 PSR.select :wash @22:42:13.45 PS.reconfigure :default @22:42:13.50 PS.seek 0.5ml @22:42:19.46 delay 5 seconds @22:42:24.48 PSR.select :air @22:42:24.60 PS.seek 2ml @22:42:40.74 PTV.dial :puck @22:42:40.76 PSV.dial PTV @22:42:41.51 PBV.dial :waste1 @22:42:41.93 PS.seek 1.5ml @22:42:47.87 delay 3 seconds @22:42:50.89 PBV.dial between PSV and waste1 @22:42:51.25 PS.seek 0ml @22:43:07.40 delay 2:00 @22:45:07.43 recoverPSRreagent08 @22:45:07.44 PTV.dial :puck @22:45:07.45 PBV.dial :waste1 @22:45:07.82 delay 30 seconds @22:45:37.85 PBV.dial between PSV and waste1 @22:45:38.22 PSR.select :air @22:45:38.25 PSV.dial PSR @22:45:39.01 PS.reconfigure :fast @22:45:39.06 PS.seek 2.5ml @22:46:02.99 PSV.dial PTV @22:46:03.76 delay 5 seconds @22:46:08.78 PSV.dial PSR @22:46:09.54 delay 5 seconds @22:46:14.56 PSV.dial PTV @22:46:15.32 delay 5 seconds @22:46:20.34 PSV.dial :air @22:46:21.11 PS.seek 0ml @22:46:44.30 Gate.power :camera,:ON @22:46:44.32 Mixing DA Substrates @22:46:44.33 PSR.select :air @22:46:44.34 PSV.dial PSR @22:46:46.33 Pulling 0.25ml of PSR.sub1 @22:46:46.37 PSR.select :sub1 @22:46:46.60 PS.reconfigure :default @22:46:46.72 PS.seek 0.25ml @22:46:50.18 delay 5 seconds @22:46:55.20 Pulling 0.25ml of PSR.sub2 @22:46:55.21 PSR.select :sub2 @22:46:55.33 PS.seek 0.5ml @22:46:58.68 delay 5 seconds @22:47:03.70 PSR.select :air @22:47:03.81 PS.seek 2ml @22:47:20.05 PTV.dial :puck @22:47:20.07 PSV.dial PTV @22:47:20.83 PBV.dial :waste1 @22:47:21.27 PS.seek 1.5ml @22:47:27.18 delay 3 seconds @22:47:30.20 PBV.dial between PSV and waste1 @22:47:30.57 PS.seek 0ml @22:47:46.71 delay 10 seconds @22:47:56.74 recoverPSRreagent08 @22:47:56.75 PTV.dial :puck @22:47:56.76 PBV.dial :waste1 @22:47:57.13 delay 30 seconds @22:48:27.16 PBV.dial between PSV and waste1 @22:48:27.53 PSR.select :air @22:48:27.55 PSV.dial PSR @22:48:28.45 PS.reconfigure :fast @22:48:28.50 PS.seek 2.5ml @22:48:52.58 PSV.dial PTV @22:48:53.33 delay 5 seconds @22:48:58.35 PSV.dial PSR @22:48:59.11 delay 5 seconds @22:49:04.14 PSV.dial PTV @22:49:04.91 delay 5 seconds @22:49:09.93 PSV.dial :air @22:49:10.71 PS.seek 0ml @22:49:34.52 Additional air clear @22:49:34.53 PSR.select :air @22:49:34.55 PSV.dial PSR @22:49:35.91 Pulling 2ml of PSR.air @22:49:35.92 PSR.select :air @22:49:35.94 PS.reconfigure :default @22:49:35.99 PS.seek 2ml @22:49:57.30 delay 5 seconds @22:50:02.32 PTV.dial :puck @22:50:02.34 PSV.dial PTV @22:50:03.10 PBV.dial :waste1 @22:50:03.53 PS.seek 1.5ml @22:50:09.46 delay 3 seconds @22:50:12.47 PBV.dial between PSV and waste1 @22:50:12.85 delay 1 second @22:50:13.87 PSV.dial :waste1 @22:50:14.96 PS.seek 0ml @22:50:31.10 Processing Puck Heater at 28C before imaging @22:50:31.11 PH.coast @22:50:31.22 Imaging da puck in Processing Clamp while storing it to tube 5 @22:50:31.40 SC.reconfigure :tight @22:50:31.45 SC.seek 5 @22:50:35.05 SC.configure :limp @22:50:35.69 Elbow.reconfigure :tight @22:50:35.81 Elbow.seek 5 @22:50:37.52 Forearm.reconfigure :tight @22:50:37.58 Forearm.seek 5 + 450 counts @22:50:38.60 SE.reconfigure :pick @22:50:38.66 SE.seek :beamBreak @22:50:40.68 SE.configure :default @22:50:40.84 Elbow.reconfigure :limp @22:50:40.90 Forearm.reconfigure :loose @22:50:40.94 Forearm.seek 5 @22:50:41.29 Elbow.hold @22:50:41.36 Elbow.reconfigure :tight @22:50:41.46 SE.seek 17.14 Pucks below Top Plate @22:50:41.94 Forearm.force -20 @22:50:41.99 Forearm.seek 5 + 292 counts @22:50:42.27 Forearm.reconfigure :tight @22:50:42.32 Forearm.seek 5 + 450 counts @22:50:42.52 SE.seek 18.14 Pucks below Top Plate @22:50:43.09 Forearm.reconfigure :loose @22:50:43.14 Forearm.seek :retract @22:50:44.06 Elbow.seek PC @22:50:46.58 Elbow.reconfigure :loose @22:50:46.68 Forearm.seek PC @22:50:47.67 Hand.close @22:50:48.46 PC.seek :open @22:50:53.25 Forearm.seek :retract @22:50:54.24 Elbow.reconfigure :tight @22:50:54.29 Elbow.seek 5 @22:50:56.90 Forearm.reconfigure :tight @22:50:56.95 Forearm.seek 5 + 450 counts @22:50:57.91 Hand.open @22:50:59.15 SE.seek 17.79 Pucks below Top Plate @22:50:59.38 SE.reconfigure :seal @22:50:59.43 SE.seek 16.83 Pucks below Top Plate @22:50:59.94 Lights, camera, ACTION! @22:50:59.98 PuckCamera: snap -bin=2,2 -auto /var/log/esp/da23dec1122h300ml.tif @22:51:19.49 Waiting >20 seconds for da to relinquish FlushPuck @22:51:24.67 SX HX9/16 (USB:25): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 21.313 seconds /var/log/esp/da23dec1122h300ml.tif: TIFF Upload Complete @22:51:24.70 PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da23dec1122h300ml15s.tif @22:51:40.31 SX HX9/16 (USB:25): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15 seconds /var/log/esp/midres/da23dec1122h300ml15s.tif: TIFF Upload Complete @22:51:40.34 PuckCamera: snap -bin=2,2 20.0 /var/log/esp/midres/da23dec1122h300ml20s.tif @22:52:00.95 SX HX9/16 (USB:25): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 20 seconds /var/log/esp/midres/da23dec1122h300ml20s.tif: TIFF Upload Complete @22:52:00.97 Gate.power :camera,:OFF @22:52:00.99 DAfans.email "21.313 sec auto-exposure",Subject: "da23dec1122h300ml images!" @22:52:01.44 Elbow.seek 5 @22:52:01.66 Forearm.seek 5 + 450 counts @22:52:01.81 SE.reconfigure :gently @22:52:01.88 SE.seek 17.55 Pucks below Top Plate @22:52:02.58 Elbow.reconfigure :limp @22:52:02.64 Forearm.reconfigure :loose @22:52:02.70 Forearm.seek 5 @22:52:02.99 Elbow.hold @22:52:03.07 Elbow.reconfigure :tight @22:52:03.13 SE.reconfigure :default @22:52:03.18 SE.seek 17.73 Pucks below Top Plate @22:52:03.59 Forearm.force -20 @22:52:03.64 Forearm.seek 5 + 250 counts @22:52:04.00 Forearm.reconfigure :tight @22:52:04.05 Forearm.seek 5 + 450 counts @22:52:04.37 SE.seek :down @22:52:05.58 Forearm.reconfigure :loose @22:52:05.69 Forearm.seek :retract @22:52:05.89 SE.stop @22:52:06.82 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @22:52:07.37 Elbow.seek FlushPuck::Garage @22:52:10.45 Elbow.reconfigure :loose @22:52:10.68 Forearm.seek :garage @22:52:11.75 Hand.close @22:52:12.65 Forearm.seek :retract @22:52:13.67 Elbow.reconfigure :tight @22:52:13.76 Elbow.seek PC @22:52:15.11 Elbow.reconfigure :loose @22:52:15.32 Forearm.reconfigure :tight @22:52:15.42 Forearm.seek PC @22:52:17.01 PC.seek :closed @22:52:21.79 Processing Clamp onSpring/offSpring for 16/26 samples, on/off torque 1.63 @22:52:21.93 Hand.open @22:52:22.72 Forearm.reconfigure :loose @22:52:22.81 Forearm.seek :retract @22:52:23.84 PSR.select :air @22:52:23.91 Storing wcr puck in Collection Clamp to tube 5 @22:52:23.94 PSV.dial PSR @22:52:24.65 Pulling 0.5ml of PSR.flush @22:52:24.72 PSR.select :flush @22:52:25.17 PS.seek 0.5ml @22:52:25.32 Elbow.reconfigure :tight @22:52:25.42 Elbow.seek 5 @22:52:28.20 Forearm.reconfigure :tight @22:52:28.30 Forearm.seek 5 + 450 counts @22:52:30.34 SE.reconfigure :pick @22:52:30.46 SE.seek :beamBreak @22:52:31.13 delay 5 seconds @22:52:32.52 SE.configure :default @22:52:32.96 Elbow.reconfigure :limp @22:52:33.07 Forearm.reconfigure :loose @22:52:33.16 Forearm.seek 5 @22:52:33.52 Elbow.hold @22:52:33.65 Elbow.reconfigure :tight @22:52:33.95 SE.seek 18.15 Pucks below Top Plate @22:52:34.67 Forearm.force -20 @22:52:34.77 Forearm.seek 5 + 255 counts @22:52:35.24 Forearm.reconfigure :tight @22:52:35.34 Forearm.seek 5 + 450 counts @22:52:35.63 SE.seek 19.15 Pucks below Top Plate @22:52:36.25 PSR.select :air @22:52:36.59 Forearm.reconfigure :loose @22:52:36.69 Forearm.seek :retract @22:52:36.76 PS.seek 2.5ml @22:52:37.71 upsync @22:52:37.76 Elbow.seek CC @22:52:39.42 Elbow.reconfigure :loose @22:52:39.53 Forearm.seek CC @22:52:40.48 Hand.close @22:52:41.09 CC.seek :open @22:52:45.85 Forearm.seek :retract @22:52:46.88 Elbow.reconfigure :tight @22:52:47.31 Elbow.seek 5 @22:52:49.13 Elbow.reconfigure :limp @22:52:49.19 Forearm.seek 5 @22:52:50.23 Elbow.hold @22:52:50.29 Elbow.reconfigure :tight @22:52:50.34 Hand.open @22:52:51.08 Forearm.force -20 @22:52:51.13 Forearm.seek 5 + 317 counts @22:52:51.44 Forearm.reconfigure :tight @22:52:51.50 Forearm.seek 5 + 450 counts @22:52:51.76 SE.seek :down @22:52:52.91 Forearm.reconfigure :loose @22:52:52.96 Forearm.seek :retract @22:52:53.19 SE.stop @22:52:53.95
Starting finalPurge @22:52:58.14 delay 1 second @22:52:59.16 PRV.dial :waste2,avoiding: :ab2 @22:53:01.05 PTV.dial :PRVmixing @22:53:02.37 PSV.dial :mixloop @22:53:03.42 PS.seek :empty @22:53:13.25
Waiting >20 seconds for psFlush to relinquish FlushPuck @22:53:29.70 PSR.select :air @22:53:29.72 PSV.dial PSR @22:53:30.76 Pulling 0.5ml of PSR.flush @22:53:30.77 PSR.select :flush @22:53:30.90 PS.seek 0.5ml @22:53:36.89 delay 5 seconds @22:53:41.91 PSR.select :air @22:53:42.03 PS.seek 2.5ml @22:54:03.26 delay 1 second @22:54:04.28 PBV.dial :waste1 @22:54:04.65 PTV.dial :mixing @22:54:05.42 PSV.dial :mixloop @22:54:06.59 PS.seek :empty @22:54:33.01 PSV.dial PSR @22:54:34.04 PSR.select :flush @22:54:34.17 PS.seek 1ml @22:54:45.26 PSR.select :air @22:54:45.77 PS.seek 2.5ml @22:55:01.95 PSV.dial PTV @22:55:02.82 PTV.dial :puck @22:55:03.56 PBV.dial :waste1 @22:55:03.74 PS.seek 0ml @22:55:30.10 PSV.dial PSR @22:55:30.84 PSR.select :flush @22:55:30.96 PS.seek 0.5ml @22:55:37.07 PSR.select :air @22:55:37.19 PS.seek 2.5ml @22:55:58.42 PSV.dial PTV @22:55:59.17 PBV.dial PSV @22:55:59.74 PS.seek 0ml @22:56:26.04 PSV.dial PBV @22:56:26.52 PS.seek 2.5ml @22:56:52.88 PSV.dial :waste1 @22:56:53.63 PS.seek 0ml @22:57:19.98 PSV.dial PSR @22:57:20.45 PSR.select :air @22:57:20.47 PS.seek 2.5ml @22:57:46.91 PSV.dial PTV @22:57:47.77 PBV.dial PSV @22:57:47.97 PS.seek 0ml @22:58:14.35 PSV.dial PBV @22:58:14.81 PS.seek 2.5ml @22:58:41.23 PSV.dial :waste1 @22:58:41.97 PS.seek 0ml @22:59:08.32 PSV.dial PSR @22:59:08.79 PSR.select :flush @22:59:08.91 PS.seek 1ml @22:59:20.01 PSR.select :air @22:59:20.12 PS.seek 2.5ml @22:59:36.29 PSV.dial PTV @22:59:37.02 PTV.dial :puck @22:59:37.10 PBV.dial :waste1 @22:59:37.65 PS.seek 0ml @23:00:04.01 PSV.dial PSR @23:00:04.77 PSR.select :flush @23:00:04.89 PS.seek 0.5ml @23:00:10.88 PSR.select :air @23:00:10.99 PS.seek 2.5ml @23:00:32.19 PSV.dial PTV @23:00:32.96 PBV.dial PSV @23:00:33.97 PS.seek 0ml @23:01:00.54 PSV.dial PBV @23:01:01.01 PS.seek 2.5ml @23:01:27.32 PSV.dial :waste1 @23:01:28.06 PS.seek 0ml @23:01:54.41 PSV.dial PSR @23:01:54.88 PSR.select :air @23:01:54.91 PS.seek 2.5ml @23:02:21.28 PSV.dial PTV @23:02:22.02 PBV.dial PSV @23:02:22.21 PS.seek 0ml @23:02:48.60 PSV.dial PBV @23:02:49.07 PS.seek 2.5ml @23:03:15.44 PSV.dial :waste1 @23:03:16.18 PS.seek 0ml @23:03:42.73
Loading FlushPuck into Collection Clamp from Processing Clamp @23:03:42.91 Elbow.seek PC @23:03:45.40 Elbow.reconfigure :loose @23:03:45.50 Forearm.seek PC @23:03:46.47 Hand.close @23:03:47.08 PC.seek :open @23:03:51.81 Forearm.seek :retract @23:03:52.78 Elbow.reconfigure :tight @23:03:52.83 Elbow.seek CC @23:03:56.48 Elbow.reconfigure :loose @23:03:56.58 Forearm.reconfigure :tight @23:03:56.62 Forearm.seek CC @23:03:57.93 CC.seek :closed @23:04:02.83 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.39 @23:04:02.90 Hand.open @23:04:03.51 Forearm.reconfigure :loose @23:04:03.56 Forearm.seek :retract @23:04:04.50 Flushing the Collection Valve::Manifold @23:04:04.63 CSV.dial CSR @23:04:05.07 Pulling 5ml of CSR.flush @23:04:05.08 CSR.select :flush @23:04:05.20 CS.seek 5ml @23:04:19.22 delay 5 seconds @23:04:24.24 CSR.select :air @23:04:24.41 CS.reconfigure :fast @23:04:24.46 CS.seek 7.5ml @23:04:31.25 CSV.dial :waste @23:04:32.56 CS.reconfigure :default @23:04:32.60 CS.seek 0ml @23:04:52.66 Flushing lower manifold's waste1 port @23:04:52.79 CSV.dial :flush @23:04:53.25 CS.reconfigure :fast @23:04:53.30 CS.seek 5ml @23:05:06.01 delay 5 seconds @23:05:11.03 CSV.dial :air @23:05:11.66 CS.seek 7ml @23:05:17.29 CSV.dial CTV @23:05:17.31
CTV.dial CSV @23:05:18.69 CBV.dial :waste1 @23:05:19.46 CS.seek 0ml @23:05:36.13 Flushing lower manifold's waste2 port @23:05:36.26 CSV.dial :flush @23:05:36.99 CS.seek 5ml @23:05:49.85 delay 5 seconds @23:05:54.87 CSV.dial :air @23:05:55.38 CS.seek 7ml @23:06:00.86 CSV.dial CTV @23:06:00.88
CTV.dial CSV @23:06:01.33 CBV.dial :waste2 @23:06:02.39 CS.seek 0ml @23:06:19.01 Flushing lower manifold's waste2b port @23:06:19.13 CSV.dial :flush @23:06:19.87 CS.seek 5ml @23:06:32.75 delay 5 seconds @23:06:37.77 CSV.dial :air @23:06:38.30 CS.seek 7ml @23:06:43.84 CSV.dial CTV @23:06:43.86
CTV.dial CSV @23:06:44.32 CBV.dial :waste2b @23:06:45.37 CS.seek 0ml @23:07:01.97 Disinfecting external Intake with kill @23:07:02.10 CSV.dial CSR @23:07:02.66 Pulling 8ml of CSR.kill @23:07:02.67 CSR.select :kill @23:07:02.78 CS.seek 8ml @23:07:22.60 delay 5 seconds @23:07:27.62 CSR.select :air @23:07:27.74 CS.seek 10ml @23:07:33.35 CSV.dial CBV @23:07:33.37
CBV.dial CSV @23:07:34.47 CTV.dial :intake @23:07:35.25 Intake.select :open @23:07:35.37 CS.seek 2ml @23:07:54.13 Intake.select :close @23:07:54.23 CTV.dial :air @23:07:54.98 CS.reconfigure :default @23:07:55.02 CS.seek 0ml @23:08:01.03 CSV.dial CSR @23:08:02.07 Pulling 5ml of CSR.flush CSR.select :flush @23:08:02.19 CS.seek 5ml @23:08:16.00 delay 5 seconds @23:08:21.02 CSR.select :air @23:08:21.14 CS.seek 10ml @23:08:34.69 CSV.dial CBV @23:08:35.84 CS.seek 0ml @23:09:02.25 Garaging FlushPuck in Collection Clamp @23:09:02.32 Elbow.reconfigure :tight @23:09:02.37 Elbow.seek CC @23:09:02.63 Elbow.reconfigure :loose @23:09:02.73 Forearm.seek CC @23:09:03.76 Hand.close @23:09:04.33 CC.seek :open @23:09:08.96 Forearm.seek :retract @23:09:09.92 Elbow.reconfigure :tight @23:09:09.97 Elbow.seek :garage @23:09:12.90 Elbow.reconfigure :loose @23:09:12.99 Forearm.reconfigure :tight @23:09:13.04 Forearm.seek :garage @23:09:14.05 Hand.open @23:09:14.75 Forearm.reconfigure :loose @23:09:15.24 Forearm.seek :retract @23:09:16.23 Completed [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] begun 21:03:31 @23:09:16.29 Asleep after completing DA_WCR_121123b assays @23:09:16.31 GoodNews.email "{1 => 33,\n 2 => 33,\n 3 => 33,\n 4 => 33,\n 5 => 20,\n 6 => 13,\n 7 => 31}\nCTD data unavailable\nCan@23:02:47, 30% flow, 17.1C, 33% humidity, 15.1psia, 13.720V, 0.351A, 202.766Ah, 4.81W\nSampled 50.0ml",Subject: "Asleep after completing DA_WCR_121123b assays" @23:09:20.60 upsync + ESPwhich DA_WCR_121223.rb + fn=DA_WCR_121223.rb + IFS=: + set /home/esp/esp2/mission . + fullPath=/home/esp/esp2/mission/DA_WCR_121223.rb + [ -f /home/esp/esp2/mission/DA_WCR_121223.rb ] + realpath /home/esp/esp2/mission/DA_WCR_121223.rb + return 0 + scriptPath=/home/esp/esp2local/mission/DA_WCR_121223.rb + exec ruby /home/esp/esp2/lib/esp.rb /home/esp/esp2local/mission/DA_WCR_121223.rb @16:02:01.03UTC12-Dec-23
ESP2 v3.42 I2C Gateway v3.20 for core bus @16:02:03.67 Configuration for short puck jake 1/11/21 by brent@mbari.org /home/esp/esp2/type/shallow/jake/configure.rb @16:02:07.34 Polling Can every 10:00 @16:02:07.52 Cleanly exited at 13:22:56UTC12-Dec-23 @16:02:07.56 Loading /home/esp/esp2local/mission/DA_WCR_121223.rb mission @16:02:07.62 GoodNews.email "Starting DA_WCR_121223 w/pucks in tube 6\n{1 => 33,\n 2 => 33,\n 3 => 33,\n 4 => 33,\n 5 => 20,\n 6 => 13,\n 7 => 31}\nCTD data unavailable\nCan@16:02:07, 31% flow, 16.4C, 33% humidity, 15.1psia, 13.744V, 0.266A, 206.739Ah, 3.66W",Subject: "DA_WCR_121223 begun" @16:02:07.65
Preparing to sleep until + 00:00:05 @16:02:07.70 Asleep til + 00:00:05 @16:02:07.72 Continuing at 16:02 @16:02:08.00 Gate.power :core,:OFF @16:02:08.01 Gate.power :raw,:OFF @16:02:08.02 Shutdown @16:02:11.56 GoodNews.email "Then, in tube 6: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_121223.rb:14\n{1 => 33,\n 2 => 33,\n 3 => 33,\n 4 => 33,\n 5 => 20,\n 6 => 13,\n 7 => 31}\nCTD data unavailable\nCan@16:02:07, 31% flow, 16.4C, 33% humidity, 15.1psia, 13.744V, 0.266A, 206.740Ah, 3.66W",Subject: "Asleep until 16:02" @16:02:12.63
Powering core bus @16:02:12.95 upsync @16:02:15.87 upsync @16:02:18.39
Gate.power :raw,:ON @16:02:19.04 CS.configure :default @16:02:19.09 PS.configure :default @16:02:19.14 SS.configure :default @16:02:19.19 CC.configure :default @16:02:19.24 PC.configure :default @16:02:19.30 Forearm.configure :loose @16:02:19.35 Elbow.configure :loose @16:02:19.42 SC.configure :loose @16:02:19.47 SE.configure :default @16:02:19.52 CH.configure :default @16:02:19.55 PH.configure :default @16:02:19.59 All dwarf controllers running firmware version 3.85 @16:02:19.70 Valve.reset @16:02:19.94 Intake.select :close @16:02:19.97 Exhaust.select :close @16:02:19.99 CTV.dial :air @16:02:20.14 PSV.dial :air @16:02:20.18 CSV.dial :waste1 @16:02:20.29
Forearm.home.seek :retract @16:02:20.69 SSV.dial :spit,avoiding: :exhaust @16:02:21.05 Hand.close @16:02:21.08
Elbow.reconfigure :loose @16:02:21.17 Elbow.jog 1500 @16:02:21.38 PS.home.seek 0ml @16:02:21.71
Elbow.reconfigure :loose @16:02:21.76 Elbow.home.seek :clear @16:02:22.68 CS.reconfigure :fast @16:02:22.71 SS.home.seek 0ml @16:02:22.80 CS.home.seek 0ml @16:02:25.03
SE.home.seek :down @16:02:25.66 SC.reconfigure :tight @16:02:25.70 SC.home.seek :initial @16:02:29.68 PS.stop @16:02:47.74
SC.configure :limp @16:02:47.80 Forearm.seek :retract @16:02:47.87 Elbow.seek :hammer @16:02:48.88 Forearm.reconfigure :tight @16:02:48.93 Forearm.seek retracted @16:02:49.57 PC.home.seek :open @16:02:49.59
CC.home.seek :open @16:02:52.97 Phase [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] @16:02:52.99 Notice.email "At 16:02:52 began in tube 6: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_121223.rb:14\n{1 => 33,\n 2 => 33,\n 3 => 33,\n 4 => 33,\n 5 => 20,\n 6 => 13,\n 7 => 31}\nCTD data unavailable\nCan@16:02:52, 31% flow, 16.4C, 33% humidity, 15.1psia, 13.671V, 0.290A, 206.744Ah, 3.97W",Subject: "Started DA_WCR_121223 phase initialPurge; da [1000, 1000] #da and wsr are run simultanelously" @16:02:53.00
Starting initialPurge @16:02:53.03 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @16:02:53.10 Hand.open @16:02:53.83 Elbow.reconfigure :tight @16:02:53.88 Elbow.seek FlushPuck::Garage @16:02:55.81 Elbow.reconfigure :loose @16:02:55.91 Forearm.reconfigure :loose @16:02:55.96 Forearm.seek :garage @16:02:56.96 Hand.close @16:02:57.70 Forearm.seek :retract @16:02:57.88 upsync @16:02:58.70
Elbow.reconfigure :tight @16:02:58.75 Elbow.seek CC @16:03:01.76 Elbow.reconfigure :loose @16:03:01.87 Forearm.reconfigure :tight @16:03:01.92 Forearm.seek CC @16:03:03.16 CC.seek :closed @16:03:07.74 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.52 @16:03:07.80 Hand.open @16:03:08.50 Forearm.reconfigure :loose @16:03:08.55 Forearm.seek :retract @16:03:09.52 Flushing the Collection Valve::Manifold @16:03:09.64 CSV.dial CSR @16:03:10.93 Pulling 5ml of CSR.flush @16:03:10.94 CSR.select :flush @16:03:11.06 CS.reconfigure :default @16:03:11.52 CS.seek 5ml @16:03:25.11 delay 5 seconds @16:03:30.12 CSR.select :air @16:03:30.29 CS.reconfigure :fast @16:03:30.34 CS.seek 7.5ml @16:03:37.10 CSV.dial :waste @16:03:38.41 CS.reconfigure :default @16:03:38.46 CS.seek 0ml @16:03:58.42 Purging external Intake @16:03:58.54 CSV.dial :flush @16:03:59.00 CS.reconfigure :fast @16:03:59.05 CS.seek 10ml @16:04:23.64 delay 5 seconds @16:04:28.66 CSV.dial CBV @16:04:28.69
CBV.dial CSV @16:04:29.45 CTV.dial :intake @16:04:30.24 Intake.select :open @16:04:30.36 CS.seek 0ml @16:04:53.85 Intake.select :close @16:04:53.96 CTV.dial :air @16:04:54.75 Flushing upper manifold @16:04:54.87 CSV.dial :flush @16:04:55.61 CS.seek 5ml @16:05:08.51 delay 5 seconds @16:05:13.53 CSV.dial :air @16:05:14.04 CS.seek 7ml @16:05:19.54 CSV.dial CBV @16:05:19.56
CBV.dial CSV @16:05:20.62 CTV.dial :air @16:05:20.70 CS.seek 0ml @16:05:37.57 delay 7 seconds @16:05:44.60 CS.seek 3ml @16:05:52.91 CSV.dial :waste @16:05:53.37 CS.seek 0ml @16:06:01.37 CSV.dial :flush @16:06:01.83 CS.seek 3ml @16:06:09.82 delay 5 seconds @16:06:14.84 CSV.dial :air @16:06:15.33 CS.seek 5ml @16:06:21.10 CSV.dial :air @16:06:21.19 CS.seek 0ml @16:06:33.38 delay 7 seconds @16:06:40.41 CS.seek 3ml @16:06:48.38 CSV.dial :waste @16:06:49.12 CS.seek 0ml @16:06:56.85 Garaging FlushPuck in Collection Clamp @16:06:56.92 Elbow.reconfigure :tight @16:06:56.97 Elbow.seek CC @16:06:57.27 Elbow.reconfigure :loose @16:06:57.37 Forearm.seek CC @16:06:58.42 Hand.close @16:06:59.06 CC.seek :open @16:07:04.09 Forearm.seek :retract @16:07:05.11 Elbow.reconfigure :tight @16:07:05.16 Elbow.seek :garage @16:07:08.12 Elbow.reconfigure :loose @16:07:08.22 Forearm.reconfigure :tight @16:07:08.27 Forearm.seek :garage @16:07:09.29 Hand.open @16:07:09.99 Forearm.reconfigure :loose @16:07:10.05 Forearm.seek :retract @16:07:11.02 da sampling at most 1000ml, wcr at most 1000ml @16:07:11.06 Loading sh1 puck from tube 6 into Collection Clamp @16:07:11.07 DAfans.email "Started da in tube 6 at 16:07:11UTC12-Dec-23\n{1 => 33,\n 2 => 33,\n 3 => 33,\n 4 => 33,\n 5 => 20,\n 6 => 13,\n 7 => 31}\nCTD data unavailable\nCan@16:02:52, 31% flow, 16.4C, 33% humidity, 15.1psia, 13.671V, 0.290A, 206.744Ah, 3.97W",Subject: "da sampling at most 1000ml, wcr at most 1000ml" @16:07:11.34
SC.reconfigure :tight @16:07:11.41 SC.seek 6 @16:07:15.59 upsync @16:07:18.09
SC.configure :limp @16:07:18.31 Elbow.reconfigure :tight @16:07:18.36 Elbow.seek 6 @16:07:20.32 Forearm.reconfigure :tight @16:07:20.38 Forearm.seek 6 + 450 counts @16:07:21.41 SE.reconfigure :pick @16:07:21.47 SE.seek :beamBreak @16:07:23.82 SE.configure :default @16:07:23.99 Elbow.seek 6 @16:07:24.18 Forearm.seek 6 + 300 counts @16:07:24.39 Forearm.reconfigure :loose @16:07:24.43 Hand.close @16:07:25.07 SE.seek :down @16:07:25.65
Forearm.seek :retract @16:07:26.67 Elbow.seek CC @16:07:27.13 SE.stop @16:07:28.32
Elbow.reconfigure :loose @16:07:28.42 Forearm.reconfigure :tight @16:07:28.47 Forearm.seek CC @16:07:29.70 CC.seek :closed @16:07:34.23 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.34 @16:07:34.24 Hand.open @16:07:34.87 Forearm.reconfigure :loose @16:07:34.92 Forearm.seek :retract @16:07:35.89 collectSample 1000ml w/bubble point of 24psi @16:07:35.90 Priming 7ml @16:07:35.91 Intake.select :close @16:07:35.92 Exhaust.select :close @16:07:35.94 SS.hold @16:07:36.15 Calibrating pressure and temperature sensors @16:07:36.18 CTV.dial :air @16:07:36.19 SSV.dial :spit,avoiding: :exhaust @16:07:36.28 SS.reconfigure :fast @16:07:36.33 SS.seek 5ml @16:07:39.24 SSV.dial CBV @16:07:39.26
CBV.dial SSV @16:07:40.61 delay 3.8 seconds @16:07:44.54 Sensor limits: Intake max 100.1psia; Outlet max 93.5psia @16:07:44.55 SSV.dial :spit @16:07:46.76 SS.seek 0ml @16:07:49.92 SSV.dial CBV @16:07:51.24 CTV.dial :intake @16:07:52.04 SSV.dial CBV @16:07:52.07
CBV.dial SSV @16:07:52.15 Allowing 40s for 7.0ml of remaining 7.0ml @16:07:52.16 Intake.select :open @16:07:54.33 Intake @14.7psia, vacuum from 9.2 to 12.2psi @16:07:54.34 SS.reconfigure :inhaling 2.20ml/s @16:07:54.39 SS.seek 7ml @16:08:00.30 Stroke completed -- waiting for pressure to equalize ... @16:08:00.31 SS.reconfigure :exhale @16:08:00.36 SS.hold @16:08:05.40 Intake.select :close @16:08:05.55 SSV.dial :exhaust @16:08:06.30 Exhaust.select :open @16:08:06.41 SS.seek 0ml @16:08:10.70 Exhaust.select :close @16:08:10.81 Sampling 1000.0ml @16:08:10.82 Intake.select :close @16:08:10.83 Exhaust.select :close @16:08:10.84 SS.reconfigure :default @16:08:10.89 SS.hold @16:08:11.09 CTV.dial :intake @16:08:11.34 SSV.dial CBV @16:08:11.36
CBV.dial SSV @16:08:12.11 Allowing 130s for 25.0ml of remaining 1000.0ml @16:08:12.12 Intake.select :open @16:08:14.29 Intake @15.4psia, vacuum from 9.9 to 12.9psi @16:08:14.30 SS.reconfigure :inhaling 2.20ml/s @16:08:14.35 SS.seek 25ml @16:08:24.67 Vacuum too high -- waiting up to 40 seconds @16:08:24.68 SS.reconfigure :exhale @16:08:24.73 SS.hold @16:08:29.96 Intake @18.4psia, vacuum from 10.0 to 13.0psi @16:08:29.98 SS.reconfigure :inhaling 1.76ml/s @16:08:30.03 SS.seek 25ml @16:08:33.70 Vacuum too high -- waiting up to 40 seconds @16:08:33.71 SS.reconfigure :exhale @16:08:33.76 SS.hold @16:08:35.19 Intake @17.8psia, vacuum from 10.0 to 13.0psi @16:08:35.20 SS.reconfigure :inhaling 1.41ml/s @16:08:35.25 SS.seek 25ml @16:08:39.09 Stroke completed -- waiting for pressure to equalize ... @16:08:39.11 SS.reconfigure :exhale @16:08:39.16 SS.hold @16:09:01.64 Intake.select :close @16:09:01.79 SSV.dial :exhaust @16:09:02.54 Exhaust.select :open @16:09:02.66 SS.seek 0ml @16:09:11.70 Exhaust.select :close @16:09:11.81 SSV.dial CBV @16:09:11.83
CBV.dial SSV @16:09:12.60 Allowing 130s for 25.0ml of remaining 975.0ml @16:09:12.61 Intake.select :open @16:09:14.77 Intake @16.0psia, vacuum from 10.0 to 13.0psi @16:09:14.78 SS.reconfigure :inhaling 1.41ml/s @16:09:14.83 SS.seek 25ml @16:09:35.23 Stroke completed -- waiting for pressure to equalize ... @16:09:35.24 SS.reconfigure :exhale @16:09:35.71 SS.hold @16:10:04.16 Intake.select :close @16:10:04.32 SSV.dial :exhaust @16:10:05.08 Exhaust.select :open @16:10:05.19 SS.seek 0ml @16:10:14.06 Exhaust.select :close @16:10:14.17 SSV.dial CBV @16:10:14.19
CBV.dial SSV @16:10:14.97 Allowing 130s for 25.0ml of remaining 950.0ml @16:10:14.98 Intake.select :open @16:10:17.15 Intake @16.0psia, vacuum from 10.0 to 13.0psi @16:10:17.17 SS.reconfigure :inhaling 1.41ml/s @16:10:17.22 SS.seek 25ml @16:10:38.04 Stroke completed -- waiting for pressure to equalize ... @16:10:38.05 SS.reconfigure :exhale @16:10:38.46 SS.hold @16:11:03.06 Intake.select :close @16:11:03.26 SSV.dial :exhaust @16:11:04.02 Exhaust.select :open @16:11:04.13 SS.seek 0ml @16:11:12.90 Exhaust.select :close @16:11:13.01 SSV.dial CBV @16:11:13.05
CBV.dial SSV @16:11:13.80 Allowing 130s for 25.0ml of remaining 925.0ml @16:11:13.81 Intake.select :open @16:11:15.97 Intake @16.1psia, vacuum from 10.0 to 13.0psi @16:11:15.99 SS.reconfigure :inhaling 1.41ml/s @16:11:16.04 SS.seek 25ml @16:11:36.67 Stroke completed -- waiting for pressure to equalize ... @16:11:36.68 SS.reconfigure :exhale @16:11:36.72 SS.hold @16:12:01.37 Intake.select :close @16:12:01.52 SSV.dial :exhaust @16:12:02.28 Exhaust.select :open @16:12:02.39 SS.seek 0ml @16:12:11.40 Exhaust.select :close @16:12:11.51 SSV.dial CBV @16:12:11.53
CBV.dial SSV @16:12:12.31 Allowing 130s for 25.0ml of remaining 900.0ml @16:12:12.32 Intake.select :open @16:12:14.49 Intake @16.2psia, vacuum from 10.0 to 13.0psi @16:12:14.51 SS.reconfigure :inhaling 1.41ml/s @16:12:14.56 SS.seek 25ml @16:12:34.79 Stroke completed -- waiting for pressure to equalize ... @16:12:34.80 SS.reconfigure :exhale @16:12:34.85 SS.hold @16:12:55.44 Intake.select :close @16:12:55.59 SSV.dial :exhaust @16:12:56.36 Exhaust.select :open @16:12:56.47 SS.seek 0ml @16:13:05.21 Exhaust.select :close @16:13:05.32 SSV.dial CBV @16:13:05.34
CBV.dial SSV @16:13:06.12 Allowing 130s for 25.0ml of remaining 875.0ml @16:13:06.13 Intake.select :open @16:13:08.30 Intake @16.2psia, vacuum from 10.0 to 13.0psi @16:13:08.31 SS.reconfigure :inhaling 1.41ml/s @16:13:08.36 SS.seek 25ml @16:13:28.92 Stroke completed -- waiting for pressure to equalize ... @16:13:28.93 SS.reconfigure :exhale @16:13:28.97 SS.hold @16:13:49.38 Intake.select :close @16:13:49.53 SSV.dial :exhaust @16:13:50.30 Exhaust.select :open @16:13:50.42 SS.seek 0ml @16:13:59.25 Exhaust.select :close @16:13:59.36 SSV.dial CBV @16:13:59.38
CBV.dial SSV @16:14:00.15 Allowing 130s for 25.0ml of remaining 850.0ml @16:14:00.16 Intake.select :open @16:14:02.33 Intake @16.2psia, vacuum from 10.0 to 13.0psi @16:14:02.35 SS.reconfigure :inhaling 1.41ml/s @16:14:02.39 SS.seek 25ml @16:14:22.93 Stroke completed -- waiting for pressure to equalize ... @16:14:22.94 SS.reconfigure :exhale @16:14:22.99 SS.hold @16:14:41.34 Intake.select :close @16:14:41.50 SSV.dial :exhaust @16:14:42.26 Exhaust.select :open @16:14:42.38 SS.seek 0ml @16:14:51.34 Exhaust.select :close @16:14:51.45 SSV.dial CBV @16:14:51.47
CBV.dial SSV @16:14:52.25 Allowing 130s for 25.0ml of remaining 825.0ml @16:14:52.26 Intake.select :open @16:14:54.42 Intake @16.2psia, vacuum from 10.0 to 13.0psi @16:14:54.43 SS.reconfigure :inhaling 1.41ml/s @16:14:54.48 SS.seek 25ml @16:15:14.79 Stroke completed -- waiting for pressure to equalize ... @16:15:14.80 SS.reconfigure :exhale @16:15:14.85 SS.hold @16:15:33.19 Intake.select :close @16:15:33.36 SSV.dial :exhaust @16:15:34.12 Exhaust.select :open @16:15:34.23 SS.seek 0ml @16:15:42.95 Exhaust.select :close @16:15:43.05 SSV.dial CBV @16:15:43.07
CBV.dial SSV @16:15:43.85 Allowing 130s for 25.0ml of remaining 800.0ml @16:15:43.87 Intake.select :open @16:15:46.03 Intake @16.2psia, vacuum from 10.0 to 13.0psi @16:15:46.05 SS.reconfigure :inhaling 1.41ml/s @16:15:46.09 SS.seek 25ml @16:16:06.65 Stroke completed -- waiting for pressure to equalize ... @16:16:06.66 SS.reconfigure :exhale @16:16:06.70 SS.hold @16:16:24.96 Intake.select :close @16:16:25.47 SSV.dial :exhaust @16:16:26.31 Exhaust.select :open @16:16:26.42 SS.seek 0ml @16:16:35.18 Exhaust.select :close @16:16:35.29 SSV.dial CBV @16:16:35.31
CBV.dial SSV @16:16:36.09 Allowing 130s for 25.0ml of remaining 775.0ml @16:16:36.10 Intake.select :open @16:16:38.27 Intake @16.2psia, vacuum from 10.0 to 13.0psi @16:16:38.29 SS.reconfigure :inhaling 1.41ml/s @16:16:38.33 SS.seek 25ml @16:16:58.82 Stroke completed -- waiting for pressure to equalize ... @16:16:58.83 SS.reconfigure :exhale @16:16:58.88 SS.hold @16:17:15.08 Intake.select :close @16:17:15.23 SSV.dial :exhaust @16:17:15.99 Exhaust.select :open @16:17:16.11 SS.seek 0ml @16:17:24.82 Exhaust.select :close @16:17:24.93 SSV.dial CBV @16:17:24.95
CBV.dial SSV @16:17:25.73 Allowing 130s for 25.0ml of remaining 750.0ml @16:17:25.74 Intake.select :open @16:17:27.90 Intake @16.2psia, vacuum from 10.0 to 13.0psi @16:17:27.91 SS.reconfigure :inhaling 1.41ml/s @16:17:27.96 SS.seek 25ml @16:17:48.72 Stroke completed -- waiting for pressure to equalize ... @16:17:48.73 SS.reconfigure :exhale @16:17:48.78 SS.hold @16:18:05.35 Intake.select :close @16:18:05.51 SSV.dial :exhaust @16:18:06.27 Exhaust.select :open @16:18:06.39 SS.seek 0ml @16:18:15.11 Exhaust.select :close @16:18:15.23 SSV.dial CBV @16:18:15.25
CBV.dial SSV @16:18:16.02 Allowing 130s for 25.0ml of remaining 725.0ml @16:18:16.03 Intake.select :open @16:18:18.20 Intake @16.3psia, vacuum from 10.0 to 13.0psi @16:18:18.21 SS.reconfigure :inhaling 1.41ml/s @16:18:18.26 SS.seek 25ml @16:18:38.94 Stroke completed -- waiting for pressure to equalize ... @16:18:38.95 SS.reconfigure :exhale @16:18:39.00 SS.hold @16:18:57.45 Intake.select :close @16:18:57.61 SSV.dial :exhaust @16:18:58.38 Exhaust.select :open @16:18:58.50 SS.seek 0ml @16:19:07.27 Exhaust.select :close @16:19:07.38 SSV.dial CBV @16:19:07.40
CBV.dial SSV @16:19:08.18 Allowing 130s for 25.0ml of remaining 700.0ml @16:19:08.19 Intake.select :open @16:19:10.36 Intake @16.2psia, vacuum from 10.0 to 13.0psi @16:19:10.37 SS.reconfigure :inhaling 1.41ml/s @16:19:10.42 SS.seek 25ml @16:19:30.89 Stroke completed -- waiting for pressure to equalize ... @16:19:30.90 SS.reconfigure :exhale @16:19:30.95 SS.hold @16:19:47.24 Intake.select :close @16:19:47.40 SSV.dial :exhaust @16:19:48.16 Exhaust.select :open @16:19:48.28 SS.seek 0ml @16:19:57.05 Exhaust.select :close @16:19:57.16 SSV.dial CBV @16:19:57.18
CBV.dial SSV @16:19:57.96 Allowing 130s for 25.0ml of remaining 675.0ml @16:19:57.97 Intake.select :open @16:20:00.14 Intake @16.3psia, vacuum from 10.0 to 13.0psi @16:20:00.15 SS.reconfigure :inhaling 1.41ml/s @16:20:00.20 SS.seek 25ml @16:20:20.88 Stroke completed -- waiting for pressure to equalize ... @16:20:20.89 SS.reconfigure :exhale @16:20:20.94 SS.hold @16:20:37.15 Intake.select :close @16:20:37.31 SSV.dial :exhaust @16:20:38.08 Exhaust.select :open @16:20:38.20 SS.seek 0ml @16:20:46.96 Exhaust.select :close @16:20:47.06 SSV.dial CBV @16:20:47.08
CBV.dial SSV @16:20:47.86 Allowing 130s for 25.0ml of remaining 650.0ml @16:20:47.88 Intake.select :open @16:20:50.04 Intake @16.2psia, vacuum from 10.0 to 13.0psi @16:20:50.05 SS.reconfigure :inhaling 1.41ml/s @16:20:50.10 SS.seek 25ml @16:21:10.75 Stroke completed -- waiting for pressure to equalize ... @16:21:10.76 SS.reconfigure :exhale @16:21:10.81 SS.hold @16:21:27.10 Intake.select :close @16:21:27.25 SSV.dial :exhaust @16:21:28.02 Exhaust.select :open @16:21:28.13 SS.seek 0ml @16:21:37.28 Exhaust.select :close @16:21:37.46 SSV.dial CBV @16:21:37.48
CBV.dial SSV @16:21:38.25 Allowing 130s for 25.0ml of remaining 625.0ml @16:21:38.27 Intake.select :open @16:21:40.43 Intake @16.4psia, vacuum from 10.0 to 13.0psi @16:21:40.45 SS.reconfigure :inhaling 1.41ml/s @16:21:40.50 SS.seek 25ml @16:22:01.02 Stroke completed -- waiting for pressure to equalize ... @16:22:01.03 SS.reconfigure :exhale @16:22:01.07 SS.hold @16:22:19.43 Intake.select :close @16:22:19.58 SSV.dial :exhaust @16:22:20.35 Exhaust.select :open @16:22:20.45 SS.seek 0ml @16:22:29.14 Exhaust.select :close @16:22:29.25 SSV.dial CBV @16:22:29.27
CBV.dial SSV @16:22:30.03 Allowing 130s for 25.0ml of remaining 600.0ml @16:22:30.04 Intake.select :open @16:22:32.21 Intake @16.3psia, vacuum from 10.0 to 13.0psi @16:22:32.22 SS.reconfigure :inhaling 1.41ml/s @16:22:32.27 SS.seek 25ml @16:22:52.47 Stroke completed -- waiting for pressure to equalize ... @16:22:52.48 SS.reconfigure :exhale @16:22:52.53 SS.hold @16:23:11.05 Intake.select :close @16:23:11.21 SSV.dial :exhaust @16:23:11.95 Exhaust.select :open @16:23:12.07 SS.seek 0ml @16:23:20.73 Exhaust.select :close @16:23:20.84 SSV.dial CBV @16:23:20.86
CBV.dial SSV @16:23:21.63 Allowing 130s for 25.0ml of remaining 575.0ml @16:23:21.64 Intake.select :open @16:23:23.81 Intake @16.3psia, vacuum from 10.0 to 13.0psi @16:23:23.82 SS.reconfigure :inhaling 1.41ml/s @16:23:23.87 SS.seek 25ml @16:23:44.47 Stroke completed -- waiting for pressure to equalize ... @16:23:44.48 SS.reconfigure :exhale @16:23:44.52 SS.hold @16:24:02.99 Intake.select :close @16:24:03.14 SSV.dial :exhaust @16:24:03.89 Exhaust.select :open @16:24:04.01 SS.seek 0ml @16:24:12.75 Exhaust.select :close @16:24:12.86 SSV.dial CBV @16:24:12.88
CBV.dial SSV @16:24:13.64 Allowing 130s for 25.0ml of remaining 550.0ml @16:24:13.66 Intake.select :open @16:24:15.82 Intake @16.3psia, vacuum from 10.0 to 13.0psi @16:24:15.83 SS.reconfigure :inhaling 1.41ml/s @16:24:15.88 SS.seek 25ml @16:24:35.49 Vacuum too high -- waiting up to 40 seconds @16:24:35.50 SS.reconfigure :exhale @16:24:35.56 SS.hold @16:24:43.19 Intake @15.9psia, vacuum from 10.0 to 13.0psi @16:24:43.20 SS.reconfigure :inhaling 1.13ml/s @16:24:43.25 SS.seek 25ml @16:24:44.81 Stroke completed -- waiting for pressure to equalize ... @16:24:44.82 SS.reconfigure :exhale @16:24:44.87 SS.hold @16:24:55.99 Intake.select :close @16:24:56.14 SSV.dial :exhaust @16:24:56.89 Exhaust.select :open @16:24:57.00 SS.seek 0ml @16:25:05.81 Exhaust.select :close @16:25:05.92 SSV.dial CBV @16:25:05.94
CBV.dial SSV @16:25:06.70 Allowing 130s for 25.0ml of remaining 525.0ml @16:25:06.71 Intake.select :open @16:25:08.88 Intake @16.3psia, vacuum from 10.0 to 13.0psi @16:25:08.90 SS.reconfigure :inhaling 1.13ml/s @16:25:08.94 SS.seek 25ml @16:25:33.60 Stroke completed -- waiting for pressure to equalize ... @16:25:33.61 SS.reconfigure :exhale @16:25:33.66 SS.hold @16:25:50.24 Intake.select :close @16:25:50.39 SSV.dial :exhaust @16:25:51.14 Exhaust.select :open @16:25:51.25 SS.seek 0ml @16:26:00.03 Exhaust.select :close @16:26:00.14 SSV.dial CBV @16:26:00.16
CBV.dial SSV @16:26:00.92 Allowing 130s for 25.0ml of remaining 500.0ml @16:26:00.93 Intake.select :open @16:26:03.10 Intake @16.2psia, vacuum from 10.0 to 13.0psi @16:26:03.11 SS.reconfigure :inhaling 1.13ml/s @16:26:03.16 SS.seek 25ml @16:26:28.04 Stroke completed -- waiting for pressure to equalize ... @16:26:28.05 SS.reconfigure :exhale @16:26:28.10 SS.hold @16:26:46.57 Intake.select :close @16:26:46.73 SSV.dial :exhaust @16:26:47.48 Exhaust.select :open @16:26:47.60 SS.seek 0ml @16:26:56.37 Exhaust.select :close @16:26:56.48 SSV.dial CBV @16:26:56.50
CBV.dial SSV @16:26:57.26 Allowing 130s for 25.0ml of remaining 475.0ml @16:26:57.28 Intake.select :open @16:26:59.44 Intake @16.4psia, vacuum from 10.0 to 13.0psi @16:26:59.45 SS.reconfigure :inhaling 1.13ml/s @16:26:59.50 SS.seek 25ml @16:27:24.03 Stroke completed -- waiting for pressure to equalize ... @16:27:24.04 SS.reconfigure :exhale @16:27:24.08 SS.hold @16:27:44.95 Intake.select :close @16:27:45.10 SSV.dial :exhaust @16:27:45.86 Exhaust.select :open @16:27:45.97 SS.seek 0ml @16:27:54.78 Exhaust.select :close @16:27:54.88 SSV.dial CBV @16:27:54.91
CBV.dial SSV @16:27:55.67 Allowing 130s for 25.0ml of remaining 450.0ml @16:27:55.68 Intake.select :open @16:27:57.85 Intake @16.2psia, vacuum from 10.0 to 13.0psi @16:27:57.86 SS.reconfigure :inhaling 1.13ml/s @16:27:57.91 SS.seek 25ml @16:28:22.74 Stroke completed -- waiting for pressure to equalize ... @16:28:22.75 SS.reconfigure :exhale @16:28:22.80 SS.hold @16:28:43.27 Intake.select :close @16:28:43.42 SSV.dial :exhaust @16:28:44.17 Exhaust.select :open @16:28:44.29 SS.seek 0ml @16:28:53.29 Exhaust.select :close @16:28:53.40 SSV.dial CBV @16:28:53.42
CBV.dial SSV @16:28:54.18 Allowing 130s for 25.0ml of remaining 425.0ml @16:28:54.19 Intake.select :open @16:28:56.36 Intake @16.4psia, vacuum from 10.0 to 13.0psi @16:28:56.38 SS.reconfigure :inhaling 1.13ml/s @16:28:56.42 SS.seek 25ml @16:29:14.20 Vacuum too high -- waiting up to 40 seconds @16:29:14.22 SS.reconfigure :exhale @16:29:14.27 SS.hold @16:29:21.84 Intake @16.8psia, vacuum from 10.0 to 13.0psi @16:29:21.86 SS.reconfigure :inhaling 0.91ml/s @16:29:22.34 SS.seek 25ml @16:29:30.51 Stroke completed -- waiting for pressure to equalize ... @16:29:30.52 SS.reconfigure :exhale @16:29:30.57 SS.hold @16:29:45.00 Intake.select :close @16:29:45.15 SSV.dial :exhaust @16:29:45.92 Exhaust.select :open @16:29:46.03 SS.seek 0ml @16:29:54.79 Exhaust.select :close @16:29:54.90 SSV.dial CBV @16:29:54.92
CBV.dial SSV @16:29:55.70 Allowing 130s for 25.0ml of remaining 400.0ml @16:29:55.71 Intake.select :open @16:29:57.88 Intake @16.3psia, vacuum from 10.0 to 13.0psi @16:29:57.89 SS.reconfigure :inhaling 0.91ml/s @16:29:57.94 SS.seek 25ml @16:30:27.85 Stroke completed -- waiting for pressure to equalize ... @16:30:27.86 SS.reconfigure :exhale @16:30:27.91 SS.hold @16:30:50.71 Intake.select :close @16:30:50.86 SSV.dial :exhaust @16:30:51.63 Exhaust.select :open @16:30:51.75 SS.seek 0ml @16:31:00.52 Exhaust.select :close @16:31:00.63 SSV.dial CBV @16:31:00.65
CBV.dial SSV @16:31:01.43 Allowing 130s for 25.0ml of remaining 375.0ml @16:31:01.44 Intake.select :open @16:31:03.61 Intake @16.4psia, vacuum from 10.0 to 13.0psi @16:31:03.62 SS.reconfigure :inhaling 0.91ml/s @16:31:03.67 SS.seek 25ml @16:31:29.04 Vacuum too high -- waiting up to 40 seconds @16:31:29.06 SS.reconfigure :exhale @16:31:29.11 SS.hold @16:31:35.86 Intake @16.2psia, vacuum from 10.0 to 13.0psi @16:31:35.88 SS.reconfigure :inhaling 0.73ml/s @16:31:35.98 SS.seek 25ml @16:31:41.55 Stroke completed -- waiting for pressure to equalize ... @16:31:41.56 SS.reconfigure :exhale @16:31:41.61 SS.hold @16:31:56.02 Intake.select :close @16:31:56.17 SSV.dial :exhaust @16:31:56.93 Exhaust.select :open @16:31:57.05 SS.seek 0ml @16:32:05.82 Exhaust.select :close @16:32:06.35 SSV.dial CBV @16:32:06.37
CBV.dial SSV @16:32:07.15 Allowing 130s for 25.0ml of remaining 350.0ml @16:32:07.16 Intake.select :open @16:32:09.33 Intake @16.4psia, vacuum from 10.0 to 13.0psi @16:32:09.35 SS.reconfigure :inhaling 0.73ml/s @16:32:09.39 SS.seek 25ml @16:32:45.63 Stroke completed -- waiting for pressure to equalize ... @16:32:45.64 SS.reconfigure :exhale @16:32:45.69 SS.hold @16:33:06.11 Intake.select :close @16:33:06.26 SSV.dial :exhaust @16:33:07.02 Exhaust.select :open @16:33:07.14 SS.seek 0ml @16:33:16.08 Exhaust.select :close @16:33:16.19 SSV.dial CBV @16:33:16.21
CBV.dial SSV @16:33:16.99 Allowing 130s for 25.0ml of remaining 325.0ml @16:33:17.00 Intake.select :open @16:33:19.17 Intake @16.4psia, vacuum from 10.0 to 13.0psi @16:33:19.53 SS.reconfigure :inhaling 0.73ml/s @16:33:19.65 SS.seek 25ml @16:33:55.58 Stroke completed -- waiting for pressure to equalize ... @16:33:55.59 SS.reconfigure :exhale @16:33:55.64 SS.hold @16:34:16.07 Intake.select :close @16:34:16.23 SSV.dial :exhaust @16:34:17.00 Exhaust.select :open @16:34:17.12 SS.seek 0ml @16:34:25.89 Exhaust.select :close @16:34:26.01 SSV.dial CBV @16:34:26.03
CBV.dial SSV @16:34:26.79 Allowing 130s for 25.0ml of remaining 300.0ml @16:34:26.80 Intake.select :open @16:34:28.96 Intake @16.6psia, vacuum from 10.0 to 13.0psi @16:34:28.97 SS.reconfigure :inhaling 0.73ml/s @16:34:29.03 SS.seek 25ml @16:34:52.01 Vacuum too high -- waiting up to 40 seconds @16:34:52.02 SS.reconfigure :exhale @16:34:52.07 SS.hold @16:34:56.62 Intake @15.9psia, vacuum from 10.0 to 13.0psi @16:34:56.64 SS.reconfigure :inhaling 0.59ml/s @16:34:56.68 SS.seek 25ml @16:35:13.69 Stroke completed -- waiting for pressure to equalize ... @16:35:13.70 SS.reconfigure :exhale @16:35:13.75 SS.hold @16:35:32.43 Intake.select :close @16:35:32.59 SSV.dial :exhaust @16:35:33.33 Exhaust.select :open @16:35:33.45 SS.seek 0ml @16:35:42.52 Exhaust.select :close @16:35:42.63 SSV.dial CBV @16:35:42.65
CBV.dial SSV @16:35:43.41 Allowing 130s for 25.0ml of remaining 275.0ml @16:35:43.42 Intake.select :open @16:35:45.59 Intake @16.4psia, vacuum from 10.0 to 13.0psi @16:35:45.61 SS.reconfigure :inhaling 0.59ml/s @16:35:45.65 SS.seek 25ml @16:36:29.95 Stroke completed -- waiting for pressure to equalize ... @16:36:29.96 SS.reconfigure :exhale @16:36:30.01 SS.hold @16:36:52.46 Intake.select :close @16:36:52.61 SSV.dial :exhaust @16:36:53.78 Exhaust.select :open @16:36:53.90 SS.seek 0ml @16:37:02.70 Exhaust.select :close @16:37:02.81 SSV.dial CBV @16:37:02.86
CBV.dial SSV @16:37:03.58 Allowing 130s for 25.0ml of remaining 250.0ml @16:37:03.59 Intake.select :open @16:37:05.76 Intake @16.5psia, vacuum from 10.0 to 13.0psi @16:37:05.77 SS.reconfigure :inhaling 0.59ml/s @16:37:05.82 SS.seek 25ml @16:37:50.65 Stroke completed -- waiting for pressure to equalize ... @16:37:50.66 SS.reconfigure :exhale @16:37:50.71 SS.hold @16:38:13.15 Intake.select :close @16:38:13.30 SSV.dial :exhaust @16:38:14.06 Exhaust.select :open @16:38:14.18 SS.seek 0ml @16:38:23.00 Exhaust.select :close @16:38:23.12 SSV.dial CBV @16:38:23.15
CBV.dial SSV @16:38:23.92 Allowing 130s for 25.0ml of remaining 225.0ml @16:38:23.93 Intake.select :open @16:38:26.10 Intake @16.7psia, vacuum from 10.0 to 13.0psi @16:38:26.12 SS.reconfigure :inhaling 0.59ml/s @16:38:26.17 SS.seek 25ml @16:39:01.66 Vacuum too high -- waiting up to 40 seconds @16:39:01.67 SS.reconfigure :exhale @16:39:01.73 SS.hold @16:39:11.59 Intake @18.3psia, vacuum from 10.0 to 13.0psi @16:39:11.61 SS.reconfigure :inhaling 0.47ml/s @16:39:11.66 SS.seek 25ml @16:39:23.68 Stroke completed -- waiting for pressure to equalize ... @16:39:23.69 SS.reconfigure :exhale @16:39:23.74 SS.hold @16:39:42.08 Intake.select :close @16:39:42.23 SSV.dial :exhaust @16:39:43.00 Exhaust.select :open @16:39:43.11 SS.seek 0ml @16:39:51.86 Exhaust.select :close @16:39:51.96 SSV.dial CBV @16:39:51.98
CBV.dial SSV @16:39:53.15 Allowing 130s for 25.0ml of remaining 200.0ml @16:39:53.17 Intake.select :open @16:39:55.34 Intake @16.6psia, vacuum from 10.0 to 13.0psi @16:39:55.35 SS.reconfigure :inhaling 0.47ml/s @16:39:55.40 SS.seek 25ml @16:40:50.99 Stroke completed -- waiting for pressure to equalize ... @16:40:51.00 SS.reconfigure :exhale @16:40:51.05 SS.hold @16:41:18.01 Intake.select :close @16:41:18.17 SSV.dial :exhaust @16:41:18.93 Exhaust.select :open @16:41:19.05 SS.seek 0ml @16:41:27.85 Exhaust.select :close @16:41:27.96 SSV.dial CBV @16:41:27.99
CBV.dial SSV @16:41:28.76 Allowing 130s for 25.0ml of remaining 175.0ml @16:41:28.77 Intake.select :open @16:41:30.94 Intake @16.4psia, vacuum from 10.0 to 13.0psi @16:41:30.95 SS.reconfigure :inhaling 0.47ml/s @16:41:31.00 SS.seek 25ml @16:42:26.38 Stroke completed -- waiting for pressure to equalize ... @16:42:26.39 SS.reconfigure :exhale @16:42:26.44 SS.hold @16:42:53.09 Intake.select :close @16:42:53.25 SSV.dial :exhaust @16:42:54.02 Exhaust.select :open @16:42:54.13 SS.seek 0ml @16:43:03.27 Exhaust.select :close @16:43:03.38 SSV.dial CBV @16:43:03.41
CBV.dial SSV @16:43:04.17 Allowing 130s for 25.0ml of remaining 150.0ml @16:43:04.18 Intake.select :open @16:43:06.34 Intake @16.6psia, vacuum from 10.0 to 13.0psi @16:43:06.35 SS.reconfigure :inhaling 0.47ml/s @16:43:06.40 SS.seek 25ml @16:43:54.19 Vacuum too high -- waiting up to 40 seconds @16:43:54.20 SS.reconfigure :exhale @16:43:54.25 SS.hold @16:44:04.00 Intake @17.0psia, vacuum from 10.0 to 13.0psi @16:44:04.01 SS.reconfigure :inhaling 0.38ml/s @16:44:04.06 SS.seek 25ml @16:44:13.22 Stroke completed -- waiting for pressure to equalize ... @16:44:13.23 SS.reconfigure :exhale @16:44:13.28 SS.hold @16:44:35.76 Intake.select :close @16:44:35.91 SSV.dial :exhaust @16:44:36.66 Exhaust.select :open @16:44:36.78 SS.seek 0ml @16:44:45.89 Exhaust.select :close @16:44:46.00 SSV.dial CBV @16:44:46.02
CBV.dial SSV @16:44:46.77 Allowing 130s for 25.0ml of remaining 125.0ml @16:44:46.79 Intake.select :open @16:44:48.95 Intake @16.6psia, vacuum from 10.0 to 13.0psi @16:44:48.97 SS.reconfigure :inhaling 0.38ml/s @16:44:49.01 SS.seek 25ml @16:45:57.32 Stroke completed -- waiting for pressure to equalize ... @16:45:57.33 SS.reconfigure :exhale @16:45:57.37 SS.hold @16:46:23.95 Intake.select :close @16:46:24.10 SSV.dial :exhaust @16:46:24.87 Exhaust.select :open @16:46:24.99 SS.seek 0ml @16:46:34.27 Exhaust.select :close @16:46:34.39 SSV.dial CBV @16:46:34.44
CBV.dial SSV @16:46:35.18 Allowing 130s for 25.0ml of remaining 100.0ml @16:46:35.19 Intake.select :open @16:46:37.36 Intake @16.7psia, vacuum from 10.0 to 13.0psi @16:46:37.38 SS.reconfigure :inhaling 0.38ml/s @16:46:37.48 SS.seek 25ml @16:47:46.34 Stroke completed -- waiting for pressure to equalize ... @16:47:46.36 SS.reconfigure :exhale @16:47:46.40 SS.hold @16:48:17.06 Intake.select :close @16:48:17.65 SSV.dial :exhaust @16:48:18.41 Exhaust.select :open @16:48:18.53 SS.seek 0ml @16:48:27.41 Exhaust.select :close @16:48:27.52 SSV.dial CBV @16:48:27.55
CBV.dial SSV @16:48:28.32 Allowing 130s for 25.0ml of remaining 75.0ml @16:48:28.33 Intake.select :open @16:48:30.49 Intake @16.6psia, vacuum from 10.0 to 13.0psi @16:48:30.51 SS.reconfigure :inhaling 0.38ml/s @16:48:30.55 SS.seek 25ml @16:49:39.08 Stroke completed -- waiting for pressure to equalize ... @16:49:39.09 SS.reconfigure :exhale @16:49:39.13 SS.hold @16:50:11.75 Intake.select :close @16:50:11.90 SSV.dial :exhaust @16:50:12.66 Exhaust.select :open @16:50:12.78 SS.seek 0ml @16:50:21.64 Exhaust.select :close @16:50:21.75 SSV.dial CBV @16:50:21.77
CBV.dial SSV @16:50:22.55 Allowing 130s for 25.0ml of remaining 50.0ml @16:50:22.56 Intake.select :open @16:50:24.73 Intake @16.8psia, vacuum from 10.0 to 13.0psi @16:50:24.74 SS.reconfigure :inhaling 0.38ml/s @16:50:24.80 SS.seek 25ml @16:51:15.71 Vacuum too high -- waiting up to 40 seconds @16:51:15.72 SS.reconfigure :exhale @16:51:15.77 SS.hold @16:51:24.43 Intake @16.1psia, vacuum from 10.0 to 13.0psi @16:51:24.44 SS.reconfigure :inhaling 0.31ml/s @16:51:24.49 SS.seek 25ml @16:51:47.79 Stroke completed -- waiting for pressure to equalize ... @16:51:47.80 SS.reconfigure :exhale @16:51:47.85 SS.hold @16:52:16.61 Intake.select :close @16:52:16.76 SSV.dial :exhaust @16:52:17.53 Exhaust.select :open @16:52:17.64 SS.seek 0ml @16:52:26.45 Exhaust.select :close @16:52:26.56 SSV.dial CBV @16:52:26.58
CBV.dial SSV @16:52:27.36 Allowing 130s for 25.0ml of remaining 25.0ml @16:52:27.37 Intake.select :open @16:52:29.53 Intake @16.7psia, vacuum from 10.0 to 13.0psi @16:52:29.55 SS.reconfigure :inhaling 0.31ml/s @16:52:29.60 SS.seek 24.98ml @16:53:58.01 Stroke completed -- waiting for pressure to equalize ... @16:53:58.02 SS.reconfigure :exhale @16:53:58.07 SS.hold @16:54:30.86 Intake.select :close @16:54:31.01 SSV.dial :exhaust @16:54:31.78 Exhaust.select :open @16:54:31.89 SS.seek 0ml @16:54:40.74 Exhaust.select :close @16:54:40.85 Sampled 1000.0ml @16:54:40.87 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @16:54:40.88 Intake.select :close @16:54:40.94 CSV.dial :waste @16:54:41.07 SSV.dial CBV,avoiding: :exhaust @16:54:41.09
CBV.dial SSV @16:54:41.17 CS.seek 0ml @16:54:41.24 CSV.dial :air @16:54:41.98 CS.seek 10ml @16:54:42.98
CTV.dial :air @16:54:43.73 delay 15 seconds @16:54:58.75 SSV.dial :spit,avoiding: :exhaust @16:55:00.07 CTV.dial CSV,avoiding: :intake @16:55:00.10
SS.seek 0ml @16:55:00.23 SSV.dial CBV,avoiding: :exhaust @16:55:07.00 SSV.dial between CBV and plug @16:55:07.48 CBV.dial between SSV and waste2 @16:55:07.91 CSV.dial between air and CTV @16:55:08.24 CS.seek 8.131ml @16:55:13.31 CTV.dial CSV,avoiding: :air @16:55:13.34
CSV.dial CTV @16:55:13.72 CS.reconfigure :pressurize @16:55:13.77 CS.seek 0ml @16:55:26.88 BubblePoint: Intake @24.2psia, Outlet @1.1psia @16:55:26.89 CBV.dial SSV @16:55:27.23 SS.reconfigure :default @16:55:27.28 SS.seek 10ml @16:55:32.92 delay 15 seconds @16:55:48.05 Evacuated: Intake @23.7psia, Outlet @23.1psia @16:55:48.06 SSV.dial :spit,avoiding: :exhaust @16:55:49.09 SS.reconfigure :exhale @16:55:49.14 SS.seek 0ml @16:55:54.15 SS.reconfigure :fast @16:55:54.20 SS.seek 5ml @16:55:56.99 SSV.dial CBV,avoiding: :exhaust @16:55:57.01
CBV.dial SSV @16:55:58.36 CTV.dial :air,avoiding: :intake @16:55:59.67 CSV.dial CTV @16:55:59.70
SS.seek 10ml @16:56:00.90 CSV.dial :air @16:56:01.36 CS.reconfigure :fast @16:56:01.41 CS.seek 0ml @16:56:02.25
SSV.dial :spit,avoiding: :exhaust @16:56:03.56 SS.reconfigure :exhale @16:56:03.61 SS.seek 0ml @16:56:08.60 SS.reconfigure :fast @16:56:08.64 SS.seek 5ml @16:56:09.55 CS.stop @16:56:11.42 SSV.dial CBV,avoiding: :exhaust @16:56:11.44
CBV.dial SSV @16:56:13.80 SSV.dial :spit,avoiding: :exhaust @16:56:15.12 SS.reconfigure :default @16:56:15.59 SS.seek 0ml @16:56:18.86 SS.stop @16:56:18.90 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @16:56:18.91 Intake.select :close @16:56:18.98 CSV.dial :waste @16:56:19.11 SSV.dial CBV,avoiding: :exhaust @16:56:19.13
CBV.dial SSV @16:56:19.76 CS.seek 0ml @16:56:19.83 CSV.dial :air @16:56:20.45
CTV.dial :air @16:56:20.53 delay 15 seconds @16:56:20.70 CS.seek 10ml @16:56:35.54
SSV.dial :spit,avoiding: :exhaust @16:56:36.88 CTV.dial CSV,avoiding: :intake @16:56:36.93
SS.reconfigure :exhale @16:56:37.01 SS.seek 0ml @16:56:37.39 SSV.dial CBV,avoiding: :exhaust @16:56:45.06 SSV.dial between CBV and plug @16:56:45.53 CBV.dial between SSV and waste2 @16:56:45.93 CSV.dial between air and CTV @16:56:46.27 CS.seek 7.258ml @16:56:53.31 CTV.dial CSV,avoiding: :air @16:56:53.33
CSV.dial CTV @16:56:53.69 CS.reconfigure :pressurize @16:56:53.73 CS.seek 0ml @16:57:05.97 BubblePoint: Intake @24.3psia, Outlet @3.6psia @16:57:05.98 CBV.dial SSV @16:57:06.31 SS.reconfigure :default @16:57:06.36 SS.seek 10ml @16:57:11.98 delay 15 seconds @16:57:27.11 Evacuated: Intake @23.9psia, Outlet @23.4psia @16:57:27.12 SSV.dial :spit,avoiding: :exhaust @16:57:28.15 SS.reconfigure :exhale @16:57:28.20 SS.seek 0ml @16:57:33.24 SS.reconfigure :fast @16:57:33.29 SS.seek 5ml @16:57:36.21 SSV.dial CBV,avoiding: :exhaust @16:57:36.24
CBV.dial SSV @16:57:37.57 CTV.dial :air,avoiding: :intake @16:57:38.90 CSV.dial CTV @16:57:38.93
SS.seek 10ml @16:57:40.06 CSV.dial :air @16:57:40.53 CS.reconfigure :fast @16:57:40.58 CS.seek 0ml @16:57:41.93
SSV.dial :spit,avoiding: :exhaust @16:57:43.31 SS.reconfigure :exhale @16:57:43.36 SS.seek 0ml @16:57:47.52 CS.stop @16:57:48.39
SS.reconfigure :fast @16:57:48.44 SS.seek 5ml @16:57:51.37 SSV.dial CBV,avoiding: :exhaust @16:57:51.40
CBV.dial SSV @16:57:53.74 SSV.dial :spit,avoiding: :exhaust @16:57:55.08 SS.reconfigure :default @16:57:55.13 SS.seek 0ml @16:57:58.24 SS.stop @16:57:58.28 Lysing: Adding 2.0ml AqMeOH @16:57:58.29 CBV.dial between CSV and waste1,avoiding: :DBV @16:57:59.79 CTV.dial CSV @16:58:01.12 CSV.dial CSR @16:58:01.91 Pulling 1ml of CSR.AqMeOH @16:58:01.92 CSR.select :AqMeOH @16:58:02.03 CS.reconfigure :default @16:58:02.08 CS.seek 1ml @16:58:05.55 delay 5 seconds @16:58:10.57 CSR.select :air @16:58:10.74 CS.seek 6ml @16:58:24.43 delay 2 seconds @16:58:26.44 CSV.dial :waste2 @16:58:27.01 CS.reconfigure :fast @16:58:27.06 CS.seek 0ml @16:58:41.36 CSV.dial CSR @16:58:41.80 Pulling 2ml of CSR.AqMeOH @16:58:41.81 CSR.select :AqMeOH @16:58:41.93 CS.reconfigure :default @16:58:41.97 CS.seek 2ml @16:58:47.99 delay 5 seconds @16:58:53.01 CSR.select :air @16:58:53.18 CS.seek 5ml @16:59:01.83 delay 2 seconds @16:59:03.85 CSV.dial CTV @16:59:04.31 CS.seek 1ml @16:59:15.66 Before lysing Collection Puck Heater at 13.7C/31.1psia @16:59:15.68 Heating Puck.from 6, :sh1 in Collection Clamp to 70C for 10:00 @16:59:15.70 CH.seek 70C,8:00 @17:01:33.08 CH.hold 10:00,70C @17:01:33.29 Begun lysing Collection Puck Heater at 68.1C/38.1psia @17:11:33.30 Ended lysing Collection Puck Heater at 70C/37.3psia @17:11:33.32 CS.seek 0ml @17:11:36.77 CTV.dial between spare and air,avoiding: :intake @17:11:37.81 CSV.dial :air @17:11:38.26 CS.seek 5ml @17:11:52.16 CSV.dial CTV @17:11:52.62 delay 2 seconds @17:11:54.64 CSV.dial :air @17:11:55.09 delay 2 seconds @17:11:57.10 CSV.dial CTV @17:11:57.56 delay 2 seconds @17:11:59.57 CSV.dial :air @17:12:00.03 delay 2 seconds @17:12:02.05 CS.seek 0ml @17:12:15.79 Puck @82.4psia after final lysis pressurization @17:12:15.80 Recovering Lysate into Collection syringe @17:12:15.81 CSV.dial CBV @17:12:16.84 CBV.dial CSV @17:12:17.22 CS.seek 5ml @17:12:30.82 delay 5 seconds @17:12:35.84 CTV.dial :air @17:12:36.37 CS.seek 6.999ml @17:12:42.48 delay 5 seconds @17:12:47.50 CSV.dial :air2 @17:12:47.94 CS.seek 10ml @17:12:56.48 CSV.dial between air and CTV,avoiding: CBV @17:12:57.64 Lysing Complete @17:12:57.71 Storing sh1 puck in Collection Clamp to tube 5 @17:12:57.88 SC.reconfigure :tight @17:12:57.93 SC.seek 5 @17:13:01.56 SC.configure :limp @17:13:01.77 Elbow.reconfigure :tight @17:13:01.81 Elbow.seek 5 @17:13:03.53 Forearm.reconfigure :tight @17:13:03.58 Forearm.seek 5 + 450 counts @17:13:04.65 SE.reconfigure :pick @17:13:04.70 SE.seek :beamBreak @17:13:06.90 SE.configure :default @17:13:07.13 Elbow.reconfigure :limp @17:13:07.18 Forearm.reconfigure :loose @17:13:07.23 Forearm.seek 5 @17:13:07.47 Elbow.hold @17:13:07.54 Elbow.reconfigure :tight @17:13:07.64 SE.seek 19.16 Pucks below Top Plate @17:13:08.24 Forearm.force -20 @17:13:08.28 Forearm.seek 5 + 302 counts @17:13:08.56 Forearm.reconfigure :tight @17:13:08.61 Forearm.seek 5 + 450 counts @17:13:08.81 SE.seek 20.16 Pucks below Top Plate @17:13:09.41 Forearm.reconfigure :loose @17:13:09.46 Forearm.seek :retract @17:13:10.40 Elbow.seek CC @17:13:12.04 Elbow.reconfigure :loose @17:13:12.14 Forearm.seek CC @17:13:13.08 Hand.close @17:13:13.63 CC.seek :open @17:13:18.37 Forearm.seek :retract @17:13:19.34 Elbow.reconfigure :tight @17:13:19.39 Elbow.seek 5 @17:13:21.15 Elbow.reconfigure :limp @17:13:21.20 Forearm.seek 5 @17:13:22.26 Elbow.hold @17:13:22.32 Elbow.reconfigure :tight @17:13:22.37 Hand.open @17:13:23.12 Forearm.force -20 @17:13:23.17 Forearm.seek 5 + 311 counts @17:13:23.90 Forearm.reconfigure :tight @17:13:23.95 Forearm.seek 5 + 450 counts @17:13:24.22 SE.seek :down @17:13:25.36
Forearm.reconfigure :loose @17:13:25.41 Forearm.seek :retract @17:13:25.59 SE.stop @17:13:26.39
Tranferring DA @17:13:26.40 Beginning samplePassC @17:13:26.41 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @17:13:26.60 Elbow.seek FlushPuck::Garage @17:13:28.46 Elbow.reconfigure :loose @17:13:28.56 Forearm.seek :garage @17:13:29.55 Hand.close @17:13:30.25 Forearm.seek :retract @17:13:31.24 Elbow.reconfigure :tight @17:13:31.29 Elbow.seek CC @17:13:34.27 Elbow.reconfigure :loose @17:13:34.37 Forearm.reconfigure :tight @17:13:34.42 Forearm.seek CC @17:13:35.66 CC.seek :closed @17:13:40.19 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.39 @17:13:40.26 Hand.open @17:13:40.94 Forearm.reconfigure :loose @17:13:40.99 Forearm.seek :retract @17:13:42.36 PSV.dial PSR @17:13:43.80 Pulling 1.5ml of PSR.flush @17:13:43.81 PSR.select :flush @17:13:43.93 PS.seek 1.5ml @17:14:00.04 delay 5 seconds @17:14:05.06 PSV.dial :waste1 @17:14:05.53 PS.seek 0ml @17:14:21.73 PSV.dial PSR @17:14:22.20 PSR.select :air @17:14:22.32 PS.seek 2.5ml @17:14:48.74 PRV.dial CBV,avoiding: :ab2 @17:14:48.75
CBV.dial PRV @17:14:50.52 PSV.dial :mixloop @17:14:51.58 CTV.dial :air,avoiding: :intake @17:14:51.80 PTV.dial :PRVmixing @17:14:53.14 PS.seek 0ml @17:15:19.45 PSV.dial :air @17:15:19.92 PS.seek 2.5ml @17:15:46.23 PSV.dial :mixloop @17:15:46.72 PS.seek 0ml @17:16:13.10 PSV.dial :air @17:16:13.70 PS.seek 2.5ml @17:16:40.10 PSV.dial :mixloop @17:16:40.69 PS.seek 0ml @17:17:07.01 Garaging FlushPuck in Collection Clamp @17:17:07.08 Elbow.reconfigure :tight @17:17:07.13 Elbow.seek CC @17:17:07.42 Elbow.reconfigure :loose @17:17:07.53 Forearm.seek CC @17:17:08.58 Hand.close @17:17:09.26 CC.seek :open @17:17:13.90 Forearm.seek :retract @17:17:14.94 Elbow.reconfigure :tight @17:17:14.99 Elbow.seek :garage @17:17:17.89 Elbow.reconfigure :loose @17:17:17.99 Forearm.reconfigure :tight @17:17:18.41 Forearm.seek :garage @17:17:19.50 Hand.open @17:17:20.19 Forearm.reconfigure :loose @17:17:20.24 Forearm.seek :retract @17:17:21.27 PSV.dial :mixloop @17:17:21.28 PTV.dial :PRVmixing @17:17:21.35 PRV.dial CBV @17:17:21.38
CBV.dial PRV @17:17:21.56 CTV.dial :air,avoiding: :intake @17:17:21.57 Loading lyfil puck from tube 6 into Collection Clamp @17:17:21.82 SC.reconfigure :tight @17:17:21.86 SC.seek 6 @17:17:25.44 SC.configure :limp @17:17:25.66 Elbow.reconfigure :tight @17:17:25.71 Elbow.seek 6 @17:17:27.67 Forearm.reconfigure :tight @17:17:27.72 Forearm.seek 6 + 450 counts @17:17:28.72 SE.reconfigure :pick @17:17:28.78 SE.seek :beamBreak @17:17:31.08 SE.configure :default @17:17:31.24 Elbow.seek 6 @17:17:31.42 Forearm.seek 6 + 300 counts @17:17:31.66 Forearm.reconfigure :loose @17:17:31.71 Hand.close @17:17:32.39 SE.seek :down @17:17:32.59
Forearm.seek :retract @17:17:33.56 Elbow.seek CC @17:17:34.48 SE.stop @17:17:35.17
Elbow.reconfigure :loose @17:17:35.27 Forearm.reconfigure :tight @17:17:35.75 Forearm.seek CC @17:17:37.09 CC.seek :closed @17:17:41.61 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.16 @17:17:41.62 Hand.open @17:17:42.31 Forearm.reconfigure :loose @17:17:42.36 Forearm.seek :retract @17:17:43.35 CTV.dial CSV,avoiding: :intake @17:17:44.66 CSV.dial CTV @17:17:44.99 PS.seek 2.5ml @17:18:11.44 CS.seek 4.5ml @17:18:26.28 delay 8 seconds @17:18:34.31 PSV.dial :air @17:18:34.88 delay 2 seconds @17:18:36.90 PSV.dial :waste2 @17:18:37.67 PS.seek 0ml @17:19:04.00 CBV.dial between PRV and waste1 @17:19:04.33 CSV.dial :air @17:19:04.80 CS.seek 10ml @17:19:19.75 CSV.dial CTV @17:19:20.21 CS.seek 0ml @17:19:46.88 CBV.dial :waste1 @17:19:47.19 delay 5 seconds @17:19:52.21 CTV.dial :air,avoiding: :intake @17:19:53.62 Storing lyfil puck in Collection Clamp to tube 5 @17:19:53.80 SC.reconfigure :tight @17:19:53.85 SC.seek 5 @17:19:57.39 SC.configure :limp @17:19:58.04 Elbow.reconfigure :tight @17:19:58.11 Elbow.seek 5 @17:19:59.89 Forearm.reconfigure :tight @17:19:59.94 Forearm.seek 5 + 450 counts @17:20:01.11 SE.reconfigure :pick @17:20:01.17 SE.seek :beamBreak @17:20:02.95 SE.configure :default @17:20:03.12 Elbow.reconfigure :limp @17:20:03.17 Forearm.reconfigure :loose @17:20:03.21 Forearm.seek 5 @17:20:03.50 Elbow.hold @17:20:03.57 Elbow.reconfigure :tight @17:20:03.67 SE.seek 20.16 Pucks below Top Plate @17:20:04.22 Forearm.force -20 @17:20:04.26 Forearm.seek 5 + 308 counts @17:20:04.54 Forearm.reconfigure :tight @17:20:04.59 Forearm.seek 5 + 450 counts @17:20:04.79 SE.seek 21.16 Pucks below Top Plate @17:20:05.38 Forearm.reconfigure :loose @17:20:05.43 Forearm.seek :retract @17:20:06.36 Elbow.seek CC @17:20:08.08 Elbow.reconfigure :loose @17:20:08.18 Forearm.seek CC @17:20:09.12 Hand.close @17:20:09.75 CC.seek :open @17:20:14.49 Forearm.seek :retract @17:20:15.46 Elbow.reconfigure :tight @17:20:15.50 Elbow.seek 5 @17:20:17.70 Elbow.reconfigure :limp @17:20:17.75 Forearm.seek 5 @17:20:18.74 Elbow.hold @17:20:18.80 Elbow.reconfigure :tight @17:20:18.85 Hand.open @17:20:19.63 Forearm.force -20 @17:20:19.67 Forearm.seek 5 + 313 counts @17:20:19.98 Forearm.reconfigure :tight @17:20:20.03 Forearm.seek 5 + 450 counts @17:20:20.29 SE.seek :down @17:20:21.42
Forearm.reconfigure :loose @17:20:21.47 Forearm.seek :retract @17:20:21.54 SE.stop @17:20:22.39
PSV.dial :air @17:20:23.15 PS.seek 2.5ml @17:20:49.44 delay 1 second @17:20:50.46 PSV.dial :mixloop @17:20:50.91 delay 1 second @17:20:51.93 PSV.dial :air @17:20:52.39 delay 1 second @17:20:53.41 PSV.dial :mixloop @17:20:53.88 delay 1 second @17:20:54.89 PSV.dial :air @17:20:55.36 delay 1 second @17:20:56.38 PS.seek 0ml @17:21:22.82 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @17:21:23.00 Elbow.seek FlushPuck::Garage @17:21:24.76 Elbow.reconfigure :loose @17:21:24.86 Forearm.seek :garage @17:21:25.89 Hand.close @17:21:26.62 Forearm.seek :retract @17:21:28.05 Elbow.reconfigure :tight @17:21:28.12 Elbow.seek PC @17:21:29.39 Elbow.reconfigure :loose @17:21:29.49 Forearm.reconfigure :tight @17:21:29.54 Forearm.seek PC @17:21:30.89 PC.seek :closed @17:21:35.51 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.65 @17:21:35.58 Hand.open @17:21:36.31 Forearm.reconfigure :loose @17:21:36.36 Forearm.seek :retract @17:21:37.28 PRV.dial :air @17:21:37.75 PSV.dial :mixloop @17:21:38.29 PS.seek 0.3ml @17:21:42.09 PSV.dial PSR @17:21:43.27 Pulling 0.4ml of PSR.air @17:21:43.28 PSR.select :air @17:21:43.31 PS.seek 0.7ml @17:21:48.19 delay 3 seconds @17:21:51.21 PSR.select :air @17:21:51.23 PS.seek 2.5ml @17:22:10.47 delay 3 seconds @17:22:13.49 PSV.dial :mixloop @17:22:14.64 PTV.dial :mixing @17:22:15.41 PBV.dial :waste2 @17:22:16.37 PS.seek 0ml @17:22:42.78 Garaging FlushPuck in Processing Clamp @17:22:42.85 Elbow.reconfigure :tight @17:22:42.90 Elbow.seek PC @17:22:43.02 Elbow.reconfigure :loose @17:22:43.12 Forearm.seek PC @17:22:44.11 Hand.close @17:22:44.75 PC.seek :open @17:22:49.93 Forearm.seek :retract @17:22:50.89 Elbow.reconfigure :tight @17:22:50.94 Elbow.seek :garage @17:22:52.24 Elbow.reconfigure :loose @17:22:52.34 Forearm.reconfigure :tight @17:22:52.39 Forearm.seek :garage @17:22:53.36 Hand.open @17:22:54.06 Forearm.reconfigure :loose @17:22:54.11 Forearm.seek :retract @17:22:55.08 Loading da puck from tube 6 into Processing Clamp @17:22:55.31 SC.reconfigure :tight @17:22:55.36 SC.seek 6 @17:22:58.88 SC.configure :limp @17:22:59.09 Elbow.reconfigure :tight @17:22:59.14 Elbow.seek 6 @17:23:01.01 Forearm.reconfigure :tight @17:23:01.06 Forearm.seek 6 + 450 counts @17:23:02.09 SE.reconfigure :pick @17:23:02.15 SE.seek :beamBreak @17:23:04.63 SE.configure :default @17:23:04.79 Elbow.seek 6 @17:23:04.98 Forearm.seek 6 + 300 counts @17:23:05.17 Forearm.reconfigure :loose @17:23:05.22 Hand.close @17:23:05.90 SE.seek :down @17:23:06.10
Forearm.seek :retract @17:23:07.03 Elbow.seek PC @17:23:08.09 SE.stop @17:23:09.96
Elbow.reconfigure :loose @17:23:10.12 Forearm.reconfigure :tight @17:23:10.18 Forearm.seek PC @17:23:11.40 PC.seek :closed @17:23:16.06 Processing Clamp onSpring/offSpring for 16/26 samples, on/off torque 1.63 @17:23:16.07 Hand.open @17:23:16.69 Forearm.reconfigure :loose @17:23:16.74 Forearm.seek :retract @17:23:17.74 Heating Puck.from 6, :da in Processing Clamp to 28C for 2:00:00 @17:23:17.76 PH.seek 28C,5:00 @17:23:59.44 PH.hold 2:00:00,28C @17:23:59.49 delay 1:30 @17:25:29.54 PSR.select :air @17:25:29.56 PSV.dial PSR @17:25:30.61 Pulling 0.5ml of PSR.wash @17:25:30.62 PSR.select :wash @17:25:30.74 PS.seek 0.5ml @17:25:36.65 delay 5 seconds @17:25:41.67 PSR.select :air @17:25:41.80 PS.seek 2ml @17:25:58.00 PTV.dial :puck @17:25:58.75 PSV.dial PTV @17:25:59.51 PBV.dial :waste1 @17:26:00.91 PS.seek 1.5ml @17:26:06.81 delay 3 seconds @17:26:09.83 PBV.dial between PSV and waste1 @17:26:10.19 PS.seek 0ml @17:26:26.31 delay 5:00 @17:31:26.34 recoverPSRreagent08 @17:31:26.35 PTV.dial :puck @17:31:26.52 PBV.dial :waste1 @17:31:26.90 delay 30 seconds @17:31:56.92 PBV.dial between PSV and waste1 @17:31:57.30 PSR.select :air @17:31:57.32 PSV.dial PSR @17:31:58.20 PS.reconfigure :fast @17:31:58.25 PS.seek 2.5ml @17:32:21.99 PSV.dial PTV @17:32:22.74 delay 5 seconds @17:32:27.76 PSV.dial PSR @17:32:28.54 delay 5 seconds @17:32:33.56 PSV.dial PTV @17:32:34.32 delay 5 seconds @17:32:39.34 PSV.dial :air @17:32:40.10 PS.seek 0ml @17:33:03.27 PTV.dial :PRVmixing,via: PRV @17:33:04.63 PSV.dial :mixloop @17:33:05.10 PRV.dial :ab1 @17:33:05.85 PS.reconfigure :slow @17:33:05.90 PS.seek 0.15ml @17:33:09.68 delay 2 seconds @17:33:11.69 CBV.dial PRV @17:33:11.73
PRV.dial CBV @17:33:12.28 PS.seek 0.35ml @17:33:17.02 delay 2 seconds @17:33:19.04 CBV.dial between PRV and PSV @17:33:19.36 PRV.dial :air @17:33:19.82 PS.seek 0.9ml @17:33:31.55 PRV.dial :waste2 @17:33:32.31 PS.seek 0.5ml @17:33:40.96 addPMLReagent08 @17:33:40.97 PSV.dial :air @17:33:41.43 PS.reconfigure :default @17:33:41.48 PS.seek 2ml @17:33:57.67 PSV.dial :PML @17:33:58.14 PTV.dial :mixing @17:33:58.91 PBV.dial :waste1 @17:33:59.35 PS.seek 1.85ml @17:34:01.67 PBV.dial between PSV and waste1 @17:34:02.04 PS.seek 0ml @17:34:21.80 delay 2 seconds @17:34:23.82 Beginning samplePassC @17:34:23.83 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @17:34:24.02 Elbow.reconfigure :tight @17:34:24.07 Elbow.seek FlushPuck::Garage @17:34:25.41 Elbow.reconfigure :loose @17:34:25.51 Forearm.seek :garage @17:34:26.50 Hand.close @17:34:27.24 Forearm.seek :retract @17:34:28.26 Elbow.reconfigure :tight @17:34:28.31 Elbow.seek CC @17:34:31.22 Elbow.reconfigure :loose @17:34:31.32 Forearm.reconfigure :tight @17:34:31.37 Forearm.seek CC @17:34:32.59 CC.seek :closed @17:34:37.12 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.48 @17:34:37.19 Hand.open @17:34:37.94 Forearm.reconfigure :loose @17:34:37.98 Forearm.seek :retract @17:34:38.94 PSV.dial PSR @17:34:40.00 Pulling 1.5ml of PSR.flush @17:34:40.01 PSR.select :flush @17:34:40.13 PS.seek 1.5ml @17:34:56.30 delay 5 seconds @17:35:01.32 PSV.dial :waste1 @17:35:01.79 PS.seek 0ml @17:35:17.92 PSV.dial PSR @17:35:18.39 PSR.select :air @17:35:18.51 PS.seek 2.5ml @17:35:44.93 PRV.dial CBV,avoiding: :ab2 @17:35:44.95
CBV.dial PRV @17:35:46.60 PSV.dial :mixloop @17:35:47.65 CTV.dial :air,avoiding: :intake @17:35:47.72 PTV.dial :PRVmixing @17:35:48.49 PS.seek 0ml @17:36:14.86 PSV.dial :air @17:36:15.44 PS.seek 2.5ml @17:36:41.89 PSV.dial :mixloop @17:36:42.35 PS.seek 0ml @17:37:08.67 PSV.dial :air @17:37:09.15 PS.seek 2.5ml @17:37:35.51 PSV.dial :mixloop @17:37:35.99 PS.seek 0ml @17:38:02.27 Garaging FlushPuck in Collection Clamp @17:38:02.34 Elbow.reconfigure :tight @17:38:02.38 Elbow.seek CC @17:38:02.70 Elbow.reconfigure :loose @17:38:02.80 Forearm.seek CC @17:38:03.83 Hand.close @17:38:04.49 CC.seek :open @17:38:09.55 Forearm.seek :retract @17:38:10.58 Elbow.reconfigure :tight @17:38:10.63 Elbow.seek :garage @17:38:13.54 Elbow.reconfigure :loose @17:38:13.65 Forearm.reconfigure :tight @17:38:13.69 Forearm.seek :garage @17:38:14.63 Hand.open @17:38:15.30 Forearm.reconfigure :loose @17:38:15.35 Forearm.seek :retract @17:38:16.35 delayUntil "17:42:23.81" @17:38:16.38 Whole Cell Archive sampling 1000ml @17:38:16.40 Loading wcr puck from tube 6 into Collection Clamp @17:38:16.64 SC.reconfigure :tight @17:38:16.69 SC.seek 6 @17:38:16.76 SC.configure :limp @17:38:16.99 Elbow.reconfigure :tight @17:38:17.04 Elbow.seek 6 @17:38:19.00 Forearm.reconfigure :tight @17:38:19.06 Forearm.seek 6 + 450 counts @17:38:20.08 SE.reconfigure :pick @17:38:20.14 SE.seek :beamBreak @17:38:22.67 SE.configure :default @17:38:22.84 Elbow.seek 6 @17:38:23.02 Forearm.seek 6 + 300 counts @17:38:23.25 Forearm.reconfigure :loose @17:38:23.30 Hand.close @17:38:23.88 SE.seek :down @17:38:24.46 Forearm.seek :retract @17:38:25.51 Elbow.seek CC @17:38:26.03 SE.stop @17:38:27.12 Elbow.reconfigure :loose @17:38:27.23 Forearm.reconfigure :tight @17:38:27.28 Forearm.seek CC @17:38:28.51 CC.seek :closed @17:38:33.05 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.31 @17:38:33.06 Hand.open @17:38:33.80 Forearm.reconfigure :loose @17:38:33.85 Forearm.seek :retract @17:38:34.80 collectSample 1000ml w/bubble point of 35psi @17:38:34.82 Priming 7ml @17:38:34.83 Intake.select :close @17:38:34.84 Exhaust.select :close @17:38:34.85 SS.hold @17:38:35.07 Calibrating pressure sensors @17:38:35.08 CTV.dial :air @17:38:35.16 SSV.dial :spit,avoiding: :exhaust @17:38:35.25 SS.reconfigure :fast @17:38:35.30 SS.seek 5ml @17:38:38.02 SSV.dial CBV @17:38:38.05 CBV.dial SSV @17:38:39.41 delay 3.8 seconds @17:38:43.35 Sensor limits: Intake max 101.2psia; Outlet max 93.3psia @17:38:43.37 SSV.dial :spit @17:38:44.72 SS.seek 0ml @17:38:47.47 SSV.dial CBV @17:38:48.80 CTV.dial :intake @17:38:49.58 SSV.dial CBV @17:38:49.60 CBV.dial SSV @17:38:50.21 Allowing 40s for 7.0ml of remaining 7.0ml @17:38:50.23 Intake.select :open @17:38:52.39 Intake @14.7psia, vacuum from 9.2 to 12.2psi @17:38:52.41 SS.reconfigure :inhaling 2.20ml/s @17:38:52.46 SS.seek 7ml @17:38:59.25 Stroke completed -- waiting for pressure to equalize ... @17:38:59.27 SS.reconfigure :exhale @17:38:59.32 SS.hold @17:39:17.69 Intake.select :close @17:39:17.85 SSV.dial :exhaust @17:39:18.68 Exhaust.select :open @17:39:18.80 SS.seek 0ml @17:39:22.99 Exhaust.select :close @17:39:23.10 Sampling 1000.0ml @17:39:23.12 Intake.select :close @17:39:23.13 Exhaust.select :close @17:39:23.14 SS.reconfigure :default @17:39:23.19 SS.hold @17:39:23.41 CTV.dial :intake @17:39:23.66 SSV.dial CBV @17:39:23.68 CBV.dial SSV @17:39:24.47 Allowing 130s for 25.0ml of remaining 1000.0ml @17:39:24.49 Intake.select :open @17:39:26.66 Intake @15.7psia, vacuum from 10.0 to 13.0psi @17:39:26.68 SS.reconfigure :inhaling 2.20ml/s @17:39:26.73 SS.seek 25ml @17:39:35.15 Vacuum too high -- waiting up to 40 seconds @17:39:35.17 SS.reconfigure :exhale @17:39:35.23 SS.hold @17:39:44.19 Intake @19.9psia, vacuum from 10.0 to 13.0psi @17:39:44.21 SS.reconfigure :inhaling 1.76ml/s @17:39:44.26 SS.seek 25ml @17:39:47.18 Vacuum too high -- waiting up to 40 seconds @17:39:47.20 SS.reconfigure :exhale @17:39:47.25 SS.hold @17:39:49.09 Intake @19.8psia, vacuum from 10.0 to 13.0psi @17:39:49.11 SS.reconfigure :inhaling 1.41ml/s @17:39:49.15 SS.seek 25ml @17:39:51.53 Vacuum too high -- waiting up to 40 seconds @17:39:51.55 SS.reconfigure :exhale @17:39:51.60 SS.hold @17:39:53.38 Intake @19.6psia, vacuum from 10.0 to 13.0psi @17:39:53.40 SS.reconfigure :inhaling 1.13ml/s @17:39:53.45 SS.seek 25ml @17:39:56.87 Vacuum too high -- waiting up to 40 seconds @17:39:56.89 SS.reconfigure :exhale @17:39:56.94 SS.hold @17:39:59.08 Intake @19.9psia, vacuum from 10.0 to 13.0psi @17:39:59.10 SS.reconfigure :inhaling 0.91ml/s @17:39:59.14 SS.seek 25ml @17:40:03.34 Vacuum too high -- waiting up to 40 seconds @17:40:03.36 SS.reconfigure :exhale @17:40:03.41 SS.hold @17:40:04.87 Intake @19.8psia, vacuum from 10.0 to 13.0psi @17:40:04.89 SS.reconfigure :inhaling 0.73ml/s @17:40:04.94 SS.seek 25ml @17:40:07.06 Stroke completed -- waiting for pressure to equalize ... @17:40:07.08 SS.reconfigure :exhale @17:40:07.13 SS.hold @17:40:37.80 Intake.select :close @17:40:37.96 SSV.dial :exhaust @17:40:38.72 Exhaust.select :open @17:40:38.84 SS.seek 0ml @17:40:47.71 Exhaust.select :close @17:40:47.82 SSV.dial CBV @17:40:47.85 CBV.dial SSV @17:40:48.61 Allowing 130s for 25.0ml of remaining 975.0ml @17:40:48.63 Intake.select :open @17:40:50.80 Intake @16.0psia, vacuum from 10.0 to 13.0psi @17:40:50.82 SS.reconfigure :inhaling 0.73ml/s @17:40:50.87 SS.seek 25ml @17:41:27.48 Stroke completed -- waiting for pressure to equalize ... @17:41:27.50 SS.reconfigure :exhale @17:41:27.55 SS.hold @17:41:58.22 Intake.select :close @17:41:58.38 SSV.dial :exhaust @17:41:59.16 Exhaust.select :open @17:41:59.28 SS.seek 0ml @17:42:08.06 Exhaust.select :close @17:42:08.17 SSV.dial CBV @17:42:08.20 CBV.dial SSV @17:42:08.97 Allowing 130s for 25.0ml of remaining 950.0ml @17:42:08.99 Intake.select :open @17:42:11.15 Intake @16.2psia, vacuum from 10.0 to 13.0psi @17:42:11.17 SS.reconfigure :inhaling 0.73ml/s @17:42:11.22 SS.seek 25ml @17:42:23.82 recoverPRVreagent08 @17:42:23.84 PTV.dial :mixing @17:42:24.60 PBV.dial :waste1 @17:42:24.98 delay 30 seconds @17:42:47.56 Stroke completed -- waiting for pressure to equalize ... @17:42:47.57 SS.reconfigure :exhale @17:42:47.62 SS.hold @17:42:55.00 PBV.dial between PSV and waste1 @17:42:55.37 PSV.dial :air @17:42:55.84 PS.reconfigure :fast @17:42:55.89 PS.seek 2.5ml @17:43:16.48 Intake.select :close @17:43:16.64 SSV.dial :exhaust @17:43:17.41 Exhaust.select :open @17:43:17.53 SS.seek 0ml @17:43:19.89 PSV.dial :mixloop @17:43:20.35 delay 2 seconds @17:43:22.37 PSV.dial :air @17:43:22.84 delay 2 seconds @17:43:24.86 PSV.dial :mixloop @17:43:25.32 delay 2 seconds @17:43:26.37 Exhaust.select :close @17:43:26.49 SSV.dial CBV @17:43:26.51 CBV.dial SSV @17:43:27.30 Allowing 130s for 25.0ml of remaining 925.0ml @17:43:27.31 Intake.select :open @17:43:27.36 PSV.dial :air @17:43:27.84 PS.seek 0ml @17:43:29.48 Intake @16.3psia, vacuum from 10.0 to 13.0psi @17:43:29.50 SS.reconfigure :inhaling 0.73ml/s @17:43:29.55 SS.seek 25ml @17:43:51.54 washCycleDA #1 of 2 @17:43:51.55 PSR.select :air @17:43:51.57 PSV.dial PSR @17:43:52.91 Pulling 0.5ml of PSR.wash @17:43:52.93 PSR.select :wash @17:43:53.04 PS.reconfigure :default @17:43:53.09 PS.seek 0.5ml @17:43:59.04 delay 5 seconds @17:44:04.06 PSR.select :air @17:44:04.18 PS.seek 2ml @17:44:06.08 Stroke completed -- waiting for pressure to equalize ... @17:44:06.09 SS.reconfigure :exhale @17:44:06.14 SS.hold @17:44:20.41 PTV.dial :puck @17:44:21.16 PSV.dial PTV @17:44:21.91 PBV.dial :waste1 @17:44:22.35 PS.seek 1.5ml @17:44:28.26 delay 3 seconds @17:44:30.72 Intake.select :close @17:44:30.88 SSV.dial :exhaust @17:44:31.27 PBV.dial between PSV and waste1 @17:44:31.66 Exhaust.select :open @17:44:31.73 PS.seek 0ml @17:44:31.79 SS.seek 0ml @17:44:40.89 Exhaust.select :close @17:44:41.00 SSV.dial CBV @17:44:41.05 CBV.dial SSV @17:44:41.80 Allowing 130s for 25.0ml of remaining 900.0ml @17:44:41.81 Intake.select :open @17:44:43.98 Intake @16.2psia, vacuum from 10.0 to 13.0psi @17:44:44.00 SS.reconfigure :inhaling 0.73ml/s @17:44:44.05 SS.seek 25ml @17:44:47.85 delay 2:00 @17:45:20.12 Stroke completed -- waiting for pressure to equalize ... @17:45:20.13 SS.reconfigure :exhale @17:45:20.18 SS.hold @17:45:46.93 Intake.select :close @17:45:47.08 SSV.dial :exhaust @17:45:47.86 Exhaust.select :open @17:45:47.98 SS.seek 0ml @17:45:56.98 Exhaust.select :close @17:45:57.10 SSV.dial CBV @17:45:57.14 CBV.dial SSV @17:45:57.89 Allowing 130s for 25.0ml of remaining 875.0ml @17:45:57.90 Intake.select :open @17:46:00.07 Intake @16.1psia, vacuum from 10.0 to 13.0psi @17:46:00.09 SS.reconfigure :inhaling 0.73ml/s @17:46:00.14 SS.seek 25ml @17:46:36.34 Stroke completed -- waiting for pressure to equalize ... @17:46:36.80 SS.reconfigure :exhale @17:46:36.85 SS.hold @17:46:47.87 recoverPSRreagent08 @17:46:47.88 PTV.dial :puck @17:46:47.96 PBV.dial :waste1 @17:46:48.38 delay 30 seconds @17:46:57.39 Intake.select :close @17:46:57.55 SSV.dial :exhaust @17:46:58.32 Exhaust.select :open @17:46:58.44 SS.seek 0ml @17:47:07.68 Exhaust.select :close @17:47:07.79 SSV.dial CBV @17:47:07.82 CBV.dial SSV @17:47:08.59 Allowing 130s for 25.0ml of remaining 850.0ml @17:47:08.61 Intake.select :open @17:47:10.77 Intake @16.1psia, vacuum from 10.0 to 13.0psi @17:47:10.79 SS.reconfigure :inhaling 0.73ml/s @17:47:10.84 SS.seek 25ml @17:47:18.40 PBV.dial between PSV and waste1 @17:47:18.77 PSR.select :air @17:47:18.79 PSV.dial PSR @17:47:19.56 PS.reconfigure :fast @17:47:19.61 PS.seek 2.5ml @17:47:43.56 PSV.dial PTV @17:47:44.46 delay 5 seconds @17:47:47.12 Stroke completed -- waiting for pressure to equalize ... @17:47:47.13 SS.reconfigure :exhale @17:47:47.18 SS.hold @17:47:49.55 PSV.dial PSR @17:47:50.30 delay 5 seconds @17:47:55.32 PSV.dial PTV @17:47:56.08 delay 5 seconds @17:48:01.10 PSV.dial :air @17:48:01.87 PS.seek 0ml @17:48:09.54 Intake.select :close @17:48:09.70 SSV.dial :exhaust @17:48:10.47 Exhaust.select :open @17:48:10.59 SS.seek 0ml @17:48:19.70 Exhaust.select :close @17:48:19.81 SSV.dial CBV @17:48:19.83 CBV.dial SSV @17:48:20.61 Allowing 130s for 25.0ml of remaining 825.0ml @17:48:20.62 Intake.select :open @17:48:22.79 Intake @16.2psia, vacuum from 10.0 to 13.0psi @17:48:22.81 SS.reconfigure :inhaling 0.73ml/s @17:48:22.86 SS.seek 25ml @17:48:25.44 washCycleDA #2 of 2 @17:48:25.45 PSR.select :air @17:48:25.47 PSV.dial PSR @17:48:26.81 Pulling 0.5ml of PSR.wash @17:48:26.83 PSR.select :wash @17:48:26.95 PS.reconfigure :default @17:48:26.99 PS.seek 0.5ml @17:48:32.97 delay 5 seconds @17:48:37.99 PSR.select :air @17:48:38.11 PS.seek 2ml @17:48:54.22 PTV.dial :puck @17:48:54.23 PSV.dial PTV @17:48:54.99 PBV.dial :waste1 @17:48:55.42 PS.seek 1.5ml @17:48:58.90 Stroke completed -- waiting for pressure to equalize ... @17:48:58.91 SS.reconfigure :exhale @17:48:58.96 SS.hold @17:49:01.48 delay 3 seconds @17:49:04.49 PBV.dial between PSV and waste1 @17:49:04.88 PS.seek 0ml @17:49:20.94 delay 2:00 @17:49:21.61 Intake.select :close @17:49:21.77 SSV.dial :exhaust @17:49:22.54 Exhaust.select :open @17:49:22.66 SS.seek 0ml @17:49:31.65 Exhaust.select :close @17:49:31.76 SSV.dial CBV @17:49:31.79 CBV.dial SSV @17:49:32.57 Allowing 130s for 25.0ml of remaining 800.0ml @17:49:32.59 Intake.select :open @17:49:34.75 Intake @16.2psia, vacuum from 10.0 to 13.0psi @17:49:34.77 SS.reconfigure :inhaling 0.73ml/s @17:49:34.82 SS.seek 25ml @17:50:10.98 Stroke completed -- waiting for pressure to equalize ... @17:50:10.99 SS.reconfigure :exhale @17:50:11.04 SS.hold @17:50:33.95 Intake.select :close @17:50:34.11 SSV.dial :exhaust @17:50:34.87 Exhaust.select :open @17:50:34.99 SS.seek 0ml @17:50:43.83 Exhaust.select :close @17:50:44.41 SSV.dial CBV @17:50:44.43 CBV.dial SSV @17:50:45.20 Allowing 130s for 25.0ml of remaining 775.0ml @17:50:45.21 Intake.select :open @17:50:47.38 Intake @16.1psia, vacuum from 10.0 to 13.0psi @17:50:47.40 SS.reconfigure :inhaling 0.73ml/s @17:50:47.45 SS.seek 25ml @17:51:20.96 recoverPSRreagent08 @17:51:20.97 PTV.dial :puck @17:51:20.98 PBV.dial :waste1 @17:51:21.36 delay 30 seconds @17:51:23.89 Stroke completed -- waiting for pressure to equalize ... @17:51:23.91 SS.reconfigure :exhale @17:51:23.96 SS.hold @17:51:44.83 Intake.select :close @17:51:44.99 SSV.dial :exhaust @17:51:45.76 Exhaust.select :open @17:51:45.88 SS.seek 0ml @17:51:51.38 PBV.dial between PSV and waste1 @17:51:51.73 PSR.select :air @17:51:51.75 PSV.dial PSR @17:51:52.64 PS.reconfigure :fast @17:51:52.69 PS.seek 2.5ml @17:51:54.69 Exhaust.select :close @17:51:54.80 SSV.dial CBV @17:51:54.84 CBV.dial SSV @17:51:55.60 Allowing 130s for 25.0ml of remaining 750.0ml @17:51:55.62 Intake.select :open @17:51:57.78 Intake @16.3psia, vacuum from 10.0 to 13.0psi @17:51:57.80 SS.reconfigure :inhaling 0.73ml/s @17:51:57.85 SS.seek 25ml @17:52:16.62 PSV.dial PTV @17:52:17.37 delay 5 seconds @17:52:22.40 PSV.dial PSR @17:52:23.16 delay 5 seconds @17:52:28.18 PSV.dial PTV @17:52:28.94 delay 5 seconds @17:52:33.96 PSV.dial :air @17:52:34.25 Stroke completed -- waiting for pressure to equalize ... @17:52:34.27 SS.reconfigure :exhale @17:52:34.31 SS.hold @17:52:34.85 PS.seek 0ml @17:52:58.31 getPMLReagent08 @17:52:58.32 PRV.dial :air @17:52:58.78 delay 5 seconds @17:52:59.65 Intake.select :close @17:52:59.81 SSV.dial :exhaust @17:53:00.58 Exhaust.select :open @17:53:00.70 SS.seek 0ml @17:53:03.80 PTV.dial :PRVmixing @17:53:05.14 delay 5 seconds @17:53:09.47 Exhaust.select :close @17:53:09.58 SSV.dial CBV @17:53:09.63 CBV.dial SSV @17:53:10.15 PRV.dial :ab2 @17:53:10.37 Allowing 130s for 25.0ml of remaining 725.0ml @17:53:10.38 Intake.select :open @17:53:10.62 PTV.dial :PRVmixing @17:53:10.70 PSV.dial :mixloop @17:53:11.16 PS.reconfigure :default @17:53:11.21 PS.seek 0.3ml @17:53:12.55 Intake @16.3psia, vacuum from 10.0 to 13.0psi @17:53:12.57 SS.reconfigure :inhaling 0.73ml/s @17:53:12.62 SS.seek 25ml @17:53:15.12 delay 2 seconds @17:53:17.14 PRV.dial :air @17:53:17.68 PS.seek 0.55ml @17:53:21.09 addPMLReagent08 @17:53:21.10 PSV.dial :air @17:53:21.58 PS.seek 2ml @17:53:37.28 PSV.dial :PML @17:53:37.75 PTV.dial :mixing @17:53:38.51 PBV.dial :waste1 @17:53:39.39 PS.seek 1.85ml @17:53:41.81 PBV.dial between PSV and waste1 @17:53:42.17 PS.seek 0ml @17:53:49.03 Stroke completed -- waiting for pressure to equalize ... @17:53:49.05 SS.reconfigure :exhale @17:53:49.10 SS.hold @17:54:01.84 delay 2 seconds @17:54:03.86 delay 8:00 @17:54:13.69 Intake.select :close @17:54:13.85 SSV.dial :exhaust @17:54:14.61 Exhaust.select :open @17:54:14.73 SS.seek 0ml @17:54:23.56 Exhaust.select :close @17:54:23.67 SSV.dial CBV @17:54:23.72 CBV.dial SSV @17:54:24.46 Allowing 130s for 25.0ml of remaining 700.0ml @17:54:24.47 Intake.select :open @17:54:26.64 Intake @16.3psia, vacuum from 10.0 to 13.0psi @17:54:26.66 SS.reconfigure :inhaling 0.73ml/s @17:54:26.71 SS.seek 25ml @17:55:03.02 Stroke completed -- waiting for pressure to equalize ... @17:55:03.03 SS.reconfigure :exhale @17:55:03.08 SS.hold @17:55:29.64 Intake.select :close @17:55:29.80 SSV.dial :exhaust @17:55:30.56 Exhaust.select :open @17:55:30.67 SS.seek 0ml @17:55:39.52 Exhaust.select :close @17:55:39.63 SSV.dial CBV @17:55:39.66 CBV.dial SSV @17:55:40.42 Allowing 130s for 25.0ml of remaining 675.0ml @17:55:40.44 Intake.select :open @17:55:42.61 Intake @16.3psia, vacuum from 10.0 to 13.0psi @17:55:42.63 SS.reconfigure :inhaling 0.73ml/s @17:55:42.68 SS.seek 25ml @17:56:16.26 Vacuum too high -- waiting up to 40 seconds @17:56:16.27 SS.reconfigure :exhale @17:56:16.33 SS.hold @17:56:29.18 SS.reconfigure :inhaling 0.59ml/s @17:56:29.23 SS.seek 25ml @17:56:33.00 Stroke completed -- waiting for pressure to equalize ... @17:56:33.01 SS.reconfigure :exhale @17:56:33.06 SS.hold @17:56:51.51 Intake.select :close @17:56:51.67 SSV.dial :exhaust @17:56:52.44 Exhaust.select :open @17:56:52.56 SS.seek 0ml @17:57:01.42 Exhaust.select :close @17:57:01.54 SSV.dial CBV @17:57:01.58 CBV.dial SSV @17:57:02.33 Allowing 130s for 25.0ml of remaining 650.0ml @17:57:02.34 Intake.select :open @17:57:04.51 Intake @16.3psia, vacuum from 10.0 to 13.0psi @17:57:04.53 SS.reconfigure :inhaling 0.59ml/s @17:57:04.58 SS.seek 25ml @17:57:49.11 Stroke completed -- waiting for pressure to equalize ... @17:57:49.12 SS.reconfigure :exhale @17:57:49.17 SS.hold @17:58:17.85 Intake.select :close @17:58:18.00 SSV.dial :exhaust @17:58:18.75 Exhaust.select :open @17:58:18.87 SS.seek 0ml @17:58:28.00 Exhaust.select :close @17:58:28.11 SSV.dial CBV @17:58:28.14 CBV.dial SSV @17:58:28.91 Allowing 130s for 25.0ml of remaining 625.0ml @17:58:28.92 Intake.select :open @17:58:31.09 Intake @16.2psia, vacuum from 10.0 to 13.0psi @17:58:31.11 SS.reconfigure :inhaling 0.59ml/s @17:58:31.16 SS.seek 25ml @17:59:15.26 Stroke completed -- waiting for pressure to equalize ... @17:59:15.27 SS.reconfigure :exhale @17:59:15.32 SS.hold @17:59:46.48 Intake.select :close @17:59:46.64 SSV.dial :exhaust @17:59:47.40 Exhaust.select :open @17:59:47.52 SS.seek 0ml @17:59:56.34 Exhaust.select :close @17:59:56.45 SSV.dial CBV @17:59:56.50 CBV.dial SSV @17:59:57.25 Allowing 130s for 25.0ml of remaining 600.0ml @17:59:57.26 Intake.select :open @17:59:59.43 Intake @16.3psia, vacuum from 10.0 to 13.0psi @17:59:59.45 SS.reconfigure :inhaling 0.59ml/s @17:59:59.50 SS.seek 25ml @18:00:37.13 Vacuum too high -- waiting up to 40 seconds @18:00:37.14 SS.reconfigure :exhale @18:00:37.20 SS.hold @18:00:53.95 Intake @17.4psia, vacuum from 10.0 to 13.0psi @18:00:53.97 SS.reconfigure :inhaling 0.47ml/s @18:00:54.03 SS.seek 25ml @18:01:03.24 Stroke completed -- waiting for pressure to equalize ... @18:01:03.26 SS.reconfigure :exhale @18:01:03.30 SS.hold @18:01:25.96 Intake.select :close @18:01:26.12 SSV.dial :exhaust @18:01:26.89 Exhaust.select :open @18:01:27.00 SS.seek 0ml @18:01:35.84 Exhaust.select :close @18:01:35.95 SSV.dial CBV @18:01:35.97 CBV.dial SSV @18:01:36.75 Allowing 130s for 25.0ml of remaining 575.0ml @18:01:36.76 Intake.select :open @18:01:38.93 Intake @16.2psia, vacuum from 10.0 to 13.0psi @18:01:38.95 SS.reconfigure :inhaling 0.47ml/s @18:01:39.00 SS.seek 25ml @18:02:03.88 recoverPRVreagent08 @18:02:03.89 PTV.dial :mixing @18:02:03.96 PBV.dial :waste1 @18:02:04.33 delay 30 seconds @18:02:34.84 Stroke completed -- waiting for pressure to equalize ... @18:02:34.86 PBV.dial between PSV and waste1 @18:02:34.87 SS.reconfigure :exhale @18:02:34.98 SS.hold @18:02:35.28 PSV.dial :air @18:02:35.77 PS.reconfigure :fast @18:02:35.84 PS.seek 2.5ml @18:02:59.99 PSV.dial :mixloop @18:03:00.47 delay 2 seconds @18:03:02.49 PSV.dial :air @18:03:02.97 delay 2 seconds @18:03:04.99 PSV.dial :mixloop @18:03:05.49 delay 2 seconds @18:03:07.54 PSV.dial :air @18:03:08.15 PS.seek 0ml @18:03:11.62 Intake.select :close @18:03:11.78 SSV.dial :exhaust @18:03:12.55 Exhaust.select :open @18:03:12.67 SS.seek 0ml @18:03:21.51 Exhaust.select :close @18:03:21.62 SSV.dial CBV @18:03:21.64 CBV.dial SSV @18:03:22.42 Allowing 130s for 25.0ml of remaining 550.0ml @18:03:22.43 Intake.select :open @18:03:24.60 Intake @16.3psia, vacuum from 10.0 to 13.0psi @18:03:24.62 SS.reconfigure :inhaling 0.47ml/s @18:03:24.67 SS.seek 25ml @18:03:31.80 washCycleDA #1 of 3 @18:03:31.81 PSR.select :air @18:03:31.83 PSV.dial PSR @18:03:33.17 Pulling 0.5ml of PSR.wash @18:03:33.18 PSR.select :wash @18:03:33.30 PS.reconfigure :default @18:03:33.35 PS.seek 0.5ml @18:03:39.45 delay 5 seconds @18:03:44.47 PSR.select :air @18:03:44.59 PS.seek 2ml @18:04:00.73 PTV.dial :puck @18:04:01.48 PSV.dial PTV @18:04:02.25 PBV.dial :waste1 @18:04:02.69 PS.seek 1.5ml @18:04:08.61 delay 3 seconds @18:04:11.63 PBV.dial between PSV and waste1 @18:04:12.01 PS.seek 0ml @18:04:13.35 Vacuum too high -- waiting up to 40 seconds @18:04:13.36 SS.reconfigure :exhale @18:04:13.42 SS.hold @18:04:28.22 delay 2:00 @18:04:34.29 Intake @17.4psia, vacuum from 10.0 to 13.0psi @18:04:34.31 SS.reconfigure :inhaling 0.38ml/s @18:04:34.36 SS.seek 25ml @18:04:43.16 Stroke completed -- waiting for pressure to equalize ... @18:04:43.17 SS.reconfigure :exhale @18:04:43.22 SS.hold @18:05:05.93 Intake.select :close @18:05:06.09 SSV.dial :exhaust @18:05:06.85 Exhaust.select :open @18:05:06.97 SS.seek 0ml @18:05:16.02 Exhaust.select :close @18:05:16.14 SSV.dial CBV @18:05:16.18 CBV.dial SSV @18:05:16.93 Allowing 130s for 25.0ml of remaining 525.0ml @18:05:16.94 Intake.select :open @18:05:19.11 Intake @16.3psia, vacuum from 10.0 to 13.0psi @18:05:19.13 SS.reconfigure :inhaling 0.38ml/s @18:05:19.18 SS.seek 25ml @18:06:27.19 Stroke completed -- waiting for pressure to equalize ... @18:06:27.20 SS.reconfigure :exhale @18:06:27.70 SS.hold @18:06:28.28 recoverPSRreagent08 @18:06:28.33 PTV.dial :puck @18:06:28.53 PBV.dial :waste1 @18:06:28.90 delay 30 seconds @18:06:58.93 PBV.dial between PSV and waste1 @18:06:59.29 PSR.select :air @18:06:59.31 PSV.dial PSR @18:07:00.08 PS.reconfigure :fast @18:07:00.13 PS.seek 2.5ml @18:07:07.90 Intake.select :close @18:07:08.06 SSV.dial :exhaust @18:07:08.83 Exhaust.select :open @18:07:08.95 SS.seek 0ml @18:07:18.06 Exhaust.select :close @18:07:18.18 SSV.dial CBV @18:07:18.20 CBV.dial SSV @18:07:18.97 Allowing 130s for 25.0ml of remaining 500.0ml @18:07:18.99 Intake.select :open @18:07:21.15 Intake @16.3psia, vacuum from 10.0 to 13.0psi @18:07:21.17 SS.reconfigure :inhaling 0.38ml/s @18:07:21.22 SS.seek 25ml @18:07:24.07 PSV.dial PTV @18:07:24.84 delay 5 seconds @18:07:29.87 PSV.dial PSR @18:07:30.64 delay 5 seconds @18:07:35.66 PSV.dial PTV @18:07:36.43 delay 5 seconds @18:07:41.46 PSV.dial :air @18:07:42.22 PS.seek 0ml @18:08:05.73 washCycleDA #2 of 3 @18:08:05.74 PSR.select :air @18:08:05.76 PSV.dial PSR @18:08:06.37 Vacuum too high -- waiting up to 40 seconds @18:08:06.39 SS.reconfigure :exhale @18:08:06.44 SS.hold @18:08:07.11 Pulling 0.5ml of PSR.wash @18:08:07.12 PSR.select :wash @18:08:07.23 PS.reconfigure :default @18:08:07.33 PS.seek 0.5ml @18:08:13.31 delay 5 seconds @18:08:18.34 PSR.select :air @18:08:18.46 PS.seek 2ml @18:08:25.25 Intake @18.2psia, vacuum from 10.0 to 13.0psi @18:08:25.27 SS.reconfigure :inhaling 0.31ml/s @18:08:25.31 SS.seek 25ml @18:08:34.63 PTV.dial :puck @18:08:34.71 PSV.dial PTV @18:08:35.46 PBV.dial :waste1 @18:08:35.89 PS.seek 1.5ml @18:08:41.76 delay 3 seconds @18:08:42.62 Vacuum too high -- waiting up to 40 seconds @18:08:42.64 SS.reconfigure :exhale @18:08:42.69 SS.hold @18:08:44.78 PBV.dial between PSV and waste1 @18:08:45.14 PS.seek 0ml @18:08:51.65 Intake @18.1psia, vacuum from 10.0 to 13.0psi @18:08:51.67 SS.reconfigure :inhaling 0.25ml/s @18:08:51.72 SS.seek 25ml @18:09:01.23 delay 2:00 @18:09:06.51 Stroke completed -- waiting for pressure to equalize ... @18:09:06.52 SS.reconfigure :exhale @18:09:06.57 SS.hold @18:09:29.00 SS.reconfigure :default @18:09:29.05 SS.hold @18:09:29.12 Intake.select :close @18:09:29.28 ShallowSampler::Clogged in WCR -- Filtering too Slowly after 525.0ml @18:09:29.29 SSV.dial :exhaust @18:09:30.08 Exhaust.select :open @18:09:30.20 SS.reconfigure :exhale @18:09:30.25 SS.seek 0ml @18:09:39.31 Exhaust.select :close @18:09:39.42 Sampled 525.0ml @18:09:39.44 Drying puck before fixing for 5:00 @18:09:39.45 CBV.dial :waste2b @18:09:40.22 delay 1:00 @18:10:40.25 CSV.dial :air @18:10:40.71 CS.reconfigure :fast @18:10:40.76 CS.seek 10ml @18:11:01.26 recoverPSRreagent08 @18:11:01.27 PTV.dial :puck @18:11:01.35 PBV.dial :waste1 @18:11:01.71 delay 30 seconds @18:11:05.33 Pumping wcr puck from tube 6 up to 35psi... @18:11:05.35 CBV.dial SSV @18:11:06.62 CBV.dial between CSV and waste2b @18:11:07.59 CSV.dial between air and CTV @18:11:07.91 CS.seek 7.129ml @18:11:15.27 CTV.dial CSV,avoiding: :air @18:11:15.29 CSV.dial CTV @18:11:16.61 CS.reconfigure :pressurize @18:11:16.67 CS.seek 0ml @18:11:29.82 CBV.dial :waste2b @18:11:30.16 CS.reconfigure :default @18:11:30.21 CS.seek 0ml @18:11:31.73 PBV.dial between PSV and waste1 @18:11:32.09 PSR.select :air @18:11:32.11 PSV.dial PSR @18:11:32.87 PS.reconfigure :fast @18:11:32.92 PS.seek 2.5ml @18:11:36.48 delay 1:00 @18:11:56.85 PSV.dial PTV @18:11:57.62 delay 5 seconds @18:12:02.64 PSV.dial PSR @18:12:03.40 delay 5 seconds @18:12:08.42 PSV.dial PTV @18:12:09.18 delay 5 seconds @18:12:14.20 PSV.dial :air @18:12:14.95 PS.seek 0ml @18:12:36.51 CTV.dial :air,avoiding: :intake @18:12:37.85 Fix #1 of 2 (with RNAlater) for 2:30 @18:12:37.86 CSV.dial CSR @18:12:38.30 washCycleDA #3 of 3 @18:12:38.31 PSR.select :air @18:12:38.33 PSV.dial PSR @18:12:38.38 Pulling 2ml of CSR.RNAlater @18:12:38.40 CSR.select :RNAlater @18:12:38.52 CS.seek 2ml @18:12:39.65 Pulling 0.5ml of PSR.wash @18:12:39.66 PSR.select :wash @18:12:39.77 PS.reconfigure :default @18:12:39.82 PS.seek 0.5ml @18:12:44.45 delay 5 seconds @18:12:45.69 delay 5 seconds @18:12:49.47 CSR.select :air @18:12:49.60 CS.reconfigure :fast @18:12:49.65 CS.seek 10ml @18:12:50.71 PSR.select :air @18:12:50.83 PS.seek 2ml @18:13:06.97 PTV.dial :puck @18:13:07.05 PSV.dial PTV @18:13:07.80 PBV.dial :waste1 @18:13:08.24 PS.seek 1.5ml @18:13:09.30 Pumping wcr puck from tube 6 up to 35psi... @18:13:09.32 CBV.dial SSV @18:13:10.57 CBV.dial between CSV and waste2b @18:13:11.53 CSV.dial between air and CTV @18:13:12.14 CS.seek 7.077ml @18:13:14.20 delay 3 seconds @18:13:17.22 PBV.dial between PSV and waste1 @18:13:17.61 PS.seek 0ml @18:13:20.02 CTV.dial CSV,avoiding: :air @18:13:20.04 CSV.dial CTV @18:13:21.43 CS.reconfigure :pressurize @18:13:21.48 CS.seek 0ml @18:13:33.98 delay 2:00 @18:13:40.07 CSV.dial :air @18:13:40.55 CS.reconfigure :fast @18:13:40.60 CS.seek 10ml @18:14:05.04 CSV.dial between air and CTV @18:14:05.38 CS.seek 4.289ml @18:14:19.08 CTV.dial CSV,avoiding: :air @18:14:19.10 CSV.dial CTV @18:14:19.46 CS.reconfigure :pressurize @18:14:19.51 CS.seek 0ml @18:14:30.98 CSV.dial :air @18:14:31.44 CS.reconfigure :fast @18:14:31.49 CS.seek 10ml @18:14:55.88 CSV.dial between air and CTV @18:14:56.21 CS.seek 4.75ml @18:15:08.89 CTV.dial CSV,avoiding: :air @18:15:08.92 CSV.dial CTV @18:15:09.28 CS.reconfigure :pressurize @18:15:09.34 CS.seek 0ml @18:15:22.01 CSV.dial :air @18:15:22.47 CS.reconfigure :fast @18:15:22.52 CS.seek 10ml @18:15:34.00 recoverPSRreagent08 @18:15:34.01 PTV.dial :puck @18:15:34.08 PBV.dial :waste1 @18:15:34.46 delay 30 seconds @18:15:46.88 CSV.dial between air and CTV @18:15:47.69 CS.seek 6.065ml @18:15:57.36 CTV.dial CSV,avoiding: :air @18:15:57.39 CSV.dial CTV @18:15:57.75 CS.reconfigure :pressurize @18:15:57.81 CS.seek 0ml @18:16:04.48 PBV.dial between PSV and waste1 @18:16:04.85 PSR.select :air @18:16:04.87 PSV.dial PSR @18:16:05.63 PS.reconfigure :fast @18:16:05.68 PS.seek 2.5ml @18:16:13.92 CSV.dial :air @18:16:14.37 CS.reconfigure :default @18:16:14.42 CS.hold @18:16:14.55 ShallowSampler::Error in MAIN -- Puck.from 6, :wcr reached only 26.6 of 35psi after pumping in 40ml of gas @18:16:14.56 Waiting to Resume from Checkpoint "Pumping wcr puck from tube 6 up to 35psi..." @18:16:14.58 BadNews.email "Puck.from 6, :wcr reached only 26.6 of 35psi after pumping in 40ml of gas",Subject: "ShallowSampler::Error in MAIN" @18:16:19.34 upsync @18:16:29.60 PSV.dial PTV @18:16:30.37 delay 5 seconds @18:16:35.39 PSV.dial PSR @18:16:36.15 delay 5 seconds @18:16:41.17 PSV.dial PTV @18:16:41.94 delay 5 seconds @18:16:46.97 PSV.dial :air @18:16:47.78 PS.seek 0ml @18:17:11.11 Gate.power :camera,:ON @18:17:11.12 Mixing DA Substrates @18:17:11.13 PSR.select :air @18:17:11.15 PSV.dial PSR @18:17:12.58 Pulling 0.25ml of PSR.sub1 @18:17:12.61 PSR.select :sub1 @18:17:12.82 PS.reconfigure :default @18:17:12.93 PS.seek 0.25ml @18:17:16.39 delay 5 seconds @18:17:21.41 Pulling 0.25ml of PSR.sub2 @18:17:21.43 PSR.select :sub2 @18:17:21.54 PS.seek 0.5ml @18:17:24.91 delay 5 seconds @18:17:29.93 PSR.select :air @18:17:30.05 PS.seek 2ml @18:17:46.41 PTV.dial :puck @18:17:46.49 PSV.dial PTV @18:17:47.25 PBV.dial :waste1 @18:17:47.69 PS.seek 1.5ml @18:17:53.66 delay 3 seconds @18:17:56.68 PBV.dial between PSV and waste1 @18:17:57.55 PS.seek 0ml @18:18:13.69 delay 10 seconds @18:18:23.72 recoverPSRreagent08 @18:18:23.73 PTV.dial :puck @18:18:23.80 PBV.dial :waste1 @18:18:24.17 delay 30 seconds @18:18:54.20 PBV.dial between PSV and waste1 @18:18:54.58 PSR.select :air @18:18:54.60 PSV.dial PSR @18:18:55.38 PS.reconfigure :fast @18:18:55.44 PS.seek 2.5ml @18:19:19.62 PSV.dial PTV @18:19:20.52 delay 5 seconds @18:19:25.54 PSV.dial PSR @18:19:26.28 delay 5 seconds @18:19:31.30 PSV.dial PTV @18:19:32.06 delay 5 seconds @18:19:37.08 PSV.dial :air @18:19:37.86 PS.seek 0ml @18:20:01.78 Additional air clear @18:20:01.79 PSR.select :air @18:20:01.81 PSV.dial PSR @18:20:03.17 Pulling 2ml of PSR.air @18:20:03.18 PSR.select :air @18:20:03.20 PS.reconfigure :default @18:20:03.25 PS.seek 2ml @18:20:24.59 delay 5 seconds @18:20:29.61 PTV.dial :puck @18:20:29.70 PSV.dial PTV @18:20:30.47 PBV.dial :waste1 @18:20:30.90 PS.seek 1.5ml @18:20:36.84 delay 3 seconds @18:20:39.86 PBV.dial between PSV and waste1 @18:20:40.23 delay 1 second @18:20:41.25 PSV.dial :waste1 @18:20:42.33 PS.seek 0ml @18:20:58.55 Processing Puck Heater at 28C before imaging @18:20:58.56 PH.coast @18:20:58.68 Imaging da puck in Processing Clamp while storing it to tube 5 @18:20:58.85 SC.reconfigure :tight @18:20:58.91 SC.seek 5 @18:21:02.51 SC.configure :limp @18:21:02.74 Elbow.reconfigure :tight @18:21:02.78 Elbow.seek 5 @18:21:04.52 Forearm.reconfigure :tight @18:21:04.57 Forearm.seek 5 + 450 counts @18:21:05.72 SE.reconfigure :pick @18:21:05.78 SE.seek :beamBreak @18:21:07.50 SE.configure :default @18:21:08.10 Elbow.reconfigure :limp @18:21:08.21 Forearm.reconfigure :loose @18:21:08.26 Forearm.seek 5 @18:21:08.53 Elbow.hold @18:21:08.59 Elbow.reconfigure :tight @18:21:08.70 SE.seek 21.14 Pucks below Top Plate @18:21:09.17 Forearm.force -20 @18:21:09.22 Forearm.seek 5 + 314 counts @18:21:09.52 Forearm.reconfigure :tight @18:21:09.57 Forearm.seek 5 + 450 counts @18:21:09.77 SE.seek 22.14 Pucks below Top Plate @18:21:10.40 Forearm.reconfigure :loose @18:21:10.45 Forearm.seek :retract @18:21:11.38 Elbow.seek PC @18:21:13.88 Elbow.reconfigure :loose @18:21:13.99 Forearm.seek PC @18:21:14.94 Hand.close @18:21:15.58 PC.seek :open @18:21:20.36 Forearm.seek :retract @18:21:21.33 Elbow.reconfigure :tight @18:21:21.38 Elbow.seek 5 @18:21:23.98 Forearm.reconfigure :tight @18:21:24.03 Forearm.seek 5 + 450 counts @18:21:25.00 Hand.open @18:21:25.64 SE.seek 21.79 Pucks below Top Plate @18:21:25.90 SE.reconfigure :seal @18:21:25.96 SE.seek 20.83 Pucks below Top Plate @18:21:26.48 Lights, camera, ACTION! @18:21:26.52 PuckCamera: snap -bin=2,2 -auto /var/log/esp/da23dec1218h1000ml.tif @18:21:43.11 SX HX9/16 (USB:26): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 13.321 seconds /var/log/esp/da23dec1218h1000ml.tif: TIFF Upload Complete @18:21:43.13 PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da23dec1218h1000ml15s.tif @18:21:46.01 Waiting >20 seconds for da to relinquish FlushPuck @18:21:58.75 SX HX9/16 (USB:26): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15 seconds /var/log/esp/midres/da23dec1218h1000ml15s.tif: TIFF Upload Complete @18:21:58.77 PuckCamera: snap -bin=2,2 20.0 /var/log/esp/midres/da23dec1218h1000ml20s.tif @18:22:19.43 SX HX9/16 (USB:26): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 20 seconds /var/log/esp/midres/da23dec1218h1000ml20s.tif: TIFF Upload Complete @18:22:19.45 DAfans.email "13.321 sec auto-exposure",Subject: "da23dec1218h1000ml images!" @18:22:19.48 Gate.power :camera,:OFF @18:22:19.84 Elbow.seek 5 @18:22:20.68 Forearm.seek 5 + 450 counts @18:22:20.76 SE.reconfigure :gently @18:22:20.82 SE.seek 21.54 Pucks below Top Plate @18:22:21.49 Elbow.reconfigure :limp @18:22:21.54 Forearm.reconfigure :loose @18:22:21.61 Forearm.seek 5 @18:22:21.87 Elbow.hold @18:22:21.94 Elbow.reconfigure :tight @18:22:22.01 SE.reconfigure :default @18:22:22.06 SE.seek 21.73 Pucks below Top Plate @18:22:22.58 Forearm.force -20 @18:22:22.63 Forearm.seek 5 + 262 counts @18:22:23.13 Forearm.reconfigure :tight @18:22:23.21 Forearm.seek 5 + 450 counts @18:22:23.63 SE.seek :down @18:22:24.91 Forearm.reconfigure :loose @18:22:25.04 Forearm.seek :retract @18:22:25.13 SE.stop @18:22:26.22 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @18:22:26.63 Elbow.seek FlushPuck::Garage @18:22:28.69 Elbow.reconfigure :loose @18:22:28.91 Forearm.seek :garage @18:22:29.91 Hand.close @18:22:30.85 Forearm.seek :retract @18:22:31.87 Elbow.reconfigure :tight @18:22:31.98 Elbow.seek PC @18:22:33.32 Elbow.reconfigure :loose @18:22:33.52 Forearm.reconfigure :tight @18:22:34.70 Forearm.seek PC @18:22:36.25 PC.seek :closed @18:22:41.11 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.67 @18:22:41.24 Hand.open @18:22:41.97 Forearm.reconfigure :loose @18:22:42.06 Forearm.seek :retract @18:22:43.05 PSR.select :air @18:22:43.10 PSV.dial PSR @18:22:43.62 Pulling 0.5ml of PSR.flush @18:22:43.64 PSR.select :flush @18:22:43.82 PS.seek 0.5ml @18:22:49.89 delay 5 seconds @18:22:53.15 upsync @18:22:54.92 PSR.select :air @18:22:55.05 PS.seek 2.5ml @18:23:16.62 delay 1 second @18:23:17.64 PRV.dial :waste2,avoiding: :ab2 @18:23:19.53 PTV.dial :PRVmixing @18:23:20.84 PSV.dial :mixloop @18:23:21.87 PS.seek :empty @18:23:48.18 PSR.select :air @18:23:48.20 PSV.dial PSR @18:23:49.24 Pulling 0.5ml of PSR.flush @18:23:49.26 PSR.select :flush @18:23:49.38 PS.seek 0.5ml @18:23:55.36 delay 5 seconds @18:24:00.39 PSR.select :air @18:24:00.51 PS.seek 2.5ml @18:24:21.74 delay 1 second @18:24:22.76 PBV.dial :waste1 @18:24:23.13 PTV.dial :mixing @18:24:23.88 PSV.dial :mixloop @18:24:24.93 PS.seek :empty @18:24:51.37 PSV.dial PSR @18:24:52.41 PSR.select :flush @18:24:52.54 PS.seek 1ml @18:25:03.68 PSR.select :air @18:25:03.79 PS.seek 2.5ml @18:25:19.96 PSV.dial PTV @18:25:20.73 PTV.dial :puck @18:25:21.94 PBV.dial :waste1 @18:25:22.02 PS.seek 0ml @18:25:48.43 PSV.dial PSR @18:25:49.18 PSR.select :flush @18:25:49.30 PS.seek 0.5ml @18:25:55.27 PSR.select :air @18:25:55.39 PS.seek 2.5ml @18:26:16.71 PSV.dial PTV @18:26:17.46 PBV.dial PSV @18:26:18.03 PS.seek 0ml @18:26:44.36 PSV.dial PBV @18:26:44.84 PS.seek 2.5ml @18:27:11.18 PSV.dial :waste1 @18:27:11.92 PS.seek 0ml @18:27:38.24 PSV.dial PSR @18:27:38.71 PSR.select :air @18:27:38.73 PS.seek 2.5ml @18:28:05.05 PSV.dial PTV @18:28:05.79 PBV.dial PSV @18:28:05.98 PS.seek 0ml @18:28:32.30 PSV.dial PBV @18:28:32.78 PS.seek 2.5ml @18:28:59.17 PSV.dial :waste1 @18:29:00.04 PS.seek 0ml @18:29:26.39 PSV.dial PSR @18:29:26.85 PSR.select :flush @18:29:26.96 PS.seek 1ml @18:29:38.02 PSR.select :air @18:29:38.14 PS.seek 2.5ml @18:29:54.20 PSV.dial PTV @18:29:55.06 PTV.dial :puck @18:29:55.14 PBV.dial :waste1 @18:29:55.70 PS.seek 0ml @18:30:22.05 PSV.dial PSR @18:30:22.91 PSR.select :flush @18:30:23.03 PS.seek 0.5ml @18:30:28.95 PSR.select :air @18:30:29.07 PS.seek 2.5ml @18:30:50.30 PSV.dial PTV @18:30:51.03 PBV.dial PSV @18:30:51.60 PS.seek 0ml @18:31:18.05 PSV.dial PBV @18:31:18.52 PS.seek 2.5ml @18:31:41.33 Session Begun @18:31:45.30 PSV.dial :waste1 @18:31:46.06 PS.seek 0ml @18:31:47.92 -> showlog -500 @18:32:12.42 PSV.dial PSR @18:32:12.92 PSR.select :air @18:32:12.95 PS.seek 2.5ml @18:32:14.08 -> exit @18:32:14.10 Session Ended @18:32:39.33 PSV.dial PTV @18:32:40.06 PBV.dial PSV @18:32:40.26 PS.seek 0ml @18:33:06.67 PSV.dial PBV @18:33:07.15 PS.seek 2.5ml @18:33:33.51 PSV.dial :waste1 @18:33:34.26 PS.seek 0ml @18:34:00.61 Garaging FlushPuck in Processing Clamp @18:34:00.68 Elbow.reconfigure :tight @18:34:00.73 Elbow.seek PC @18:34:00.86 Elbow.reconfigure :loose @18:34:00.96 Forearm.seek PC @18:34:01.93 Hand.close @18:34:02.57 PC.seek :open @18:34:07.29 Forearm.seek :retract @18:34:08.30 Elbow.reconfigure :tight @18:34:08.35 Elbow.seek :garage @18:34:09.72 Elbow.reconfigure :loose @18:34:09.82 Forearm.reconfigure :tight @18:34:09.87 Forearm.seek :garage @18:34:10.89 Hand.open @18:34:11.63 Forearm.reconfigure :loose @18:34:11.68 Forearm.seek :retract @18:40:22.34 Session Begun @18:40:26.49 -> CC Collection Clamp contains wcr puck from tube 6 @18:40:29.67 -> PC Processing Clamp open @18:40:33.46 -> Flush FlushPuck is in FlushPuck::Garage @18:40:38.25 -> Puck.count @18:40:38.34 SE.seek :down @18:40:38.42 SE.stop @18:40:38.54 SC.reconfigure :tight @18:40:39.05 SC.seek 1 @18:40:52.09 SC.configure :limp @18:40:52.34 Elbow.reconfigure :tight @18:40:52.41 Elbow.seek 1 @18:40:54.31 Forearm.reconfigure :tight @18:40:54.38 Forearm.seek 1 + 450 counts @18:40:55.44 SE.reconfigure :place @18:40:55.52 SE.seek :beamBreak @18:40:56.67 SE.configure :default @18:40:56.80 SE.seek :down @18:40:57.28 SE.stop @18:40:57.94 Forearm.reconfigure :loose @18:40:58.02 Forearm.seek :retract @18:40:58.97 SC.reconfigure :tight @18:40:59.05 SC.seek 2 @18:41:02.66 SC.configure :limp @18:41:02.84 Elbow.seek 2 @18:41:03.05 Forearm.reconfigure :tight @18:41:03.13 Forearm.seek 2 + 450 counts @18:41:04.29 SE.reconfigure :place @18:41:04.38 SE.seek :beamBreak @18:41:05.62 SE.configure :default @18:41:05.76 SE.seek :down @18:41:06.23 SE.stop @18:41:06.89 Forearm.reconfigure :loose @18:41:06.96 Forearm.seek :retract @18:41:07.90 SC.reconfigure :tight @18:41:07.97 SC.seek 3 @18:41:11.60 SC.configure :limp @18:41:11.79 Elbow.seek 3 @18:41:12.22 Forearm.reconfigure :tight @18:41:12.31 Forearm.seek 3 + 450 counts @18:41:13.40 SE.reconfigure :place @18:41:13.49 SE.seek :beamBreak @18:41:14.61 SE.configure :default @18:41:15.20 SE.seek :down @18:41:15.65 SE.stop @18:41:16.34 Forearm.reconfigure :loose @18:41:16.45 Forearm.seek :retract @18:41:17.43 SC.reconfigure :tight @18:41:17.50 SC.seek 4 @18:41:21.04 SC.configure :limp @18:41:21.22 Elbow.seek 4 @18:41:21.85 Forearm.reconfigure :tight @18:41:21.93 Forearm.seek 4 + 450 counts @18:41:22.96 SE.reconfigure :place @18:41:23.05 SE.seek :beamBreak @18:41:24.18 SE.configure :default @18:41:24.32 SE.seek :down @18:41:24.77 SE.stop @18:41:25.45 Forearm.reconfigure :loose @18:41:25.53 Forearm.seek :retract @18:41:26.52 SC.reconfigure :tight @18:41:26.59 SC.seek 5 @18:41:30.20 SC.configure :limp @18:41:30.38 Elbow.seek 5 @18:41:30.70 Forearm.reconfigure :tight @18:41:30.77 Forearm.seek 5 + 450 counts @18:41:31.82 SE.reconfigure :place @18:41:31.90 SE.seek :beamBreak @18:41:33.76 SE.configure :default @18:41:33.89 SE.seek :down @18:41:35.03 Forearm.reconfigure :loose @18:41:35.11 Forearm.seek :retract @18:41:35.13 SE.stop @18:41:36.14 SC.reconfigure :tight @18:41:36.22 SC.seek 6 @18:41:39.74 SC.configure :limp @18:41:39.92 Elbow.seek 6 @18:41:40.13 Forearm.reconfigure :tight @18:41:40.21 Forearm.seek 6 + 450 counts @18:41:41.71 SE.reconfigure :place @18:41:41.79 SE.seek :beamBreak @18:41:44.59 SE.configure :default @18:41:44.73 SE.seek :down @18:41:45.86 Forearm.reconfigure :loose @18:41:45.94 Forearm.seek :retract @18:41:46.85 SE.stop @18:41:46.94 SC.reconfigure :tight @18:41:47.01 SC.seek 7 @18:41:50.56 SC.configure :limp @18:41:50.74 Elbow.seek 7 @18:41:51.05 Forearm.reconfigure :tight @18:41:51.13 Forearm.seek 7 + 450 counts @18:41:52.19 SE.reconfigure :place @18:41:52.27 SE.seek :beamBreak @18:41:53.57 SE.configure :default @18:41:53.71 SE.seek :down @18:41:54.34 SE.stop @18:41:54.84 Forearm.reconfigure :loose @18:41:54.91 Forearm.seek :retract {1 => 33, 2 => 33, 3 => 33, 4 => 33, 5 => 23, 6 => 9, 7 => 31} @18:43:19.19 -> CC.unloadPuck 5, :WCR @18:43:19.27 Puck::Warning in dave -- Unloading Puck.from 6, :wcr to Storage Carousel @18:43:19.30 Storing WCR puck in Collection Clamp to tube 5 @18:43:19.50 SC.reconfigure :tight @18:43:19.58 SC.seek 5 @18:43:26.32 SC.configure :limp @18:43:26.50 Elbow.seek 5 @18:43:27.06 Forearm.reconfigure :tight @18:43:27.13 Forearm.seek 5 + 450 counts @18:43:28.18 SE.reconfigure :pick @18:43:28.27 SE.seek :beamBreak @18:43:30.00 SE.configure :default @18:43:30.18 Elbow.reconfigure :limp @18:43:30.24 Forearm.reconfigure :loose @18:43:30.32 Forearm.seek 5 @18:43:30.62 Elbow.hold @18:43:30.70 Elbow.reconfigure :tight @18:43:30.83 SE.seek 22.15 Pucks below Top Plate @18:43:31.91 Forearm.force -20 @18:43:31.97 Forearm.seek 5 + 286 counts @18:43:32.27 Forearm.reconfigure :tight @18:43:32.35 Forearm.seek 5 + 450 counts @18:43:32.57 SE.seek 23.15 Pucks below Top Plate @18:43:33.19 Forearm.reconfigure :loose @18:43:33.27 Forearm.seek :retract @18:43:34.22 Elbow.seek CC @18:43:35.91 Elbow.reconfigure :loose @18:43:36.03 Forearm.seek CC @18:43:37.00 Hand.close @18:43:37.69 CC.seek :open @18:43:42.44 Forearm.seek :retract @18:43:43.45 Elbow.reconfigure :tight @18:43:43.53 Elbow.seek 5 @18:43:45.33 Elbow.reconfigure :limp @18:43:45.40 Forearm.seek 5 @18:43:46.46 Elbow.hold @18:43:46.55 Elbow.reconfigure :tight @18:43:46.62 Hand.open @18:43:47.44 Forearm.force -20 @18:43:47.50 Forearm.seek 5 + 315 counts @18:43:47.81 Forearm.reconfigure :tight @18:43:47.88 Forearm.seek 5 + 450 counts @18:43:48.16 SE.seek :down @18:43:49.30 Forearm.reconfigure :loose @18:43:49.38 Forearm.seek :retract @18:43:49.40 SE.stop @18:45:07.77 -> Puck.count @18:45:07.85 SE.seek :down @18:45:07.93 SE.stop @18:45:08.05 SC.reconfigure :tight @18:45:08.12 SC.seek 1 @18:45:21.09 SC.configure :limp @18:45:21.27 Elbow.seek 1 @18:45:21.48 Forearm.reconfigure :tight @18:45:21.96 Forearm.seek 1 + 450 counts @18:45:23.07 SE.reconfigure :place @18:45:23.16 SE.seek :beamBreak @18:45:24.31 SE.configure :default @18:45:24.45 SE.seek :down @18:45:24.99 SE.stop @18:45:25.59 Forearm.reconfigure :loose @18:45:25.66 Forearm.seek :retract @18:45:26.67 SC.reconfigure :tight @18:45:26.75 SC.seek 2 @18:45:30.33 SC.configure :limp @18:45:30.52 Elbow.seek 2 @18:45:30.74 Forearm.reconfigure :tight @18:45:30.81 Forearm.seek 2 + 450 counts @18:45:31.85 SE.reconfigure :place @18:45:31.93 SE.seek :beamBreak @18:45:33.03 SE.configure :default @18:45:33.16 SE.seek :down @18:45:33.63 SE.stop @18:45:34.29 Forearm.reconfigure :loose @18:45:34.37 Forearm.seek :retract @18:45:35.33 SC.reconfigure :tight @18:45:35.40 SC.seek 3 @18:45:39.01 SC.configure :limp @18:45:39.19 Elbow.seek 3 @18:45:39.53 Forearm.reconfigure :tight @18:45:39.60 Forearm.seek 3 + 450 counts @18:45:40.62 SE.reconfigure :place @18:45:40.70 SE.seek :beamBreak @18:45:41.84 SE.configure :default @18:45:41.97 SE.seek :down @18:45:42.41 SE.stop @18:45:43.10 Forearm.reconfigure :loose @18:45:43.17 Forearm.seek :retract @18:45:44.16 SC.reconfigure :tight @18:45:44.24 SC.seek 4 @18:45:47.82 SC.configure :limp @18:45:48.47 Elbow.seek 4 @18:45:49.08 Forearm.reconfigure :tight @18:45:49.16 Forearm.seek 4 + 450 counts @18:45:50.23 SE.reconfigure :place @18:45:50.32 SE.seek :beamBreak @18:45:51.45 SE.configure :default @18:45:51.59 SE.seek :down @18:45:52.04 SE.stop @18:45:52.73 Forearm.reconfigure :loose @18:45:52.80 Forearm.seek :retract @18:45:53.81 SC.reconfigure :tight @18:45:53.88 SC.seek 5 @18:45:57.43 SC.configure :limp @18:45:57.61 Elbow.seek 5 @18:45:57.91 Forearm.reconfigure :tight @18:45:57.99 Forearm.seek 5 + 450 counts @18:45:59.04 SE.reconfigure :place @18:45:59.13 SE.seek :beamBreak @18:46:00.90 SE.configure :default @18:46:01.03 SE.seek :down @18:46:02.16 SE.stop @18:46:02.24 Forearm.reconfigure :loose @18:46:02.31 Forearm.seek :retract @18:46:03.30 SC.reconfigure :tight @18:46:03.37 SC.seek 6 @18:46:06.90 SC.configure :limp @18:46:07.08 Elbow.seek 6 @18:46:07.29 Forearm.reconfigure :tight @18:46:07.36 Forearm.seek 6 + 450 counts @18:46:08.39 SE.reconfigure :place @18:46:08.47 SE.seek :beamBreak @18:46:11.26 SE.configure :default @18:46:11.39 SE.seek :down @18:46:12.53 Forearm.reconfigure :loose @18:46:12.60 Forearm.seek :retract @18:46:13.58 SE.stop @18:46:13.99 SC.reconfigure :tight @18:46:14.06 SC.seek 7 @18:46:17.62 SC.configure :limp @18:46:17.80 Elbow.seek 7 @18:46:18.36 Forearm.reconfigure :tight @18:46:18.44 Forearm.seek 7 + 450 counts @18:46:19.54 SE.reconfigure :place @18:46:19.63 SE.seek :beamBreak @18:46:20.98 SE.configure :default @18:46:21.11 SE.seek :down @18:46:21.72 SE.stop @18:46:22.24 Forearm.reconfigure :loose @18:46:22.31 Forearm.seek :retract {1 => 33, 2 => 33, 3 => 33, 4 => 33, 5 => 24, 6 => 9, 7 => 31} @18:48:24.37 -> exit @18:48:24.39 Session Ended @18:49:50.69 Session Begun @18:50:01.69 -> abort! 'mission complete' @18:50:01.70 CmdServer::Client::Error in dave -- Aborting Mission: mission complete @18:50:01.74 BadNews.email "mission complete",Subject: "DA_WCR_121223 ABORTED!" # @18:50:06.40 upsync @18:50:06.49 Session Ended + ESPwhich DA_WCR_121323.rb + fn=DA_WCR_121323.rb + IFS=: + set /home/esp/esp2/mission . + fullPath=/home/esp/esp2/mission/DA_WCR_121323.rb + [ -f /home/esp/esp2/mission/DA_WCR_121323.rb ] + realpath /home/esp/esp2/mission/DA_WCR_121323.rb + return 0 + scriptPath=/home/esp/esp2local/mission/DA_WCR_121323.rb + exec ruby /home/esp/esp2/lib/esp.rb /home/esp/esp2local/mission/DA_WCR_121323.rb @16:42:09.81UTC13-Dec-23
ESP2 v3.42 I2C Gateway v3.20 for core bus @16:42:12.51 Configuration for short puck jake 1/11/21 by brent@mbari.org /home/esp/esp2/type/shallow/jake/configure.rb @16:42:16.27 Polling Can every 10:00 @16:42:16.45 Cleanly exited at 13:11:28UTC13-Dec-23 @16:42:16.50 Loading /home/esp/esp2local/mission/DA_WCR_121323.rb mission @16:42:16.56 GoodNews.email "Starting DA_WCR_121323 w/pucks in tube 6\n{1 => 33,\n 2 => 33,\n 3 => 33,\n 4 => 33,\n 5 => 24,\n 6 => 9,\n 7 => 31}\nCTD data unavailable\nCan@16:42:16, 30% flow, 16.9C, 33% humidity, 15.1psia, 13.754V, 0.254A, 213.845Ah, 3.50W",Subject: "DA_WCR_121323 begun" @16:42:16.60
Preparing to sleep until + 00:00:05 @16:42:16.63 Asleep til + 00:00:05 @16:42:16.65 Continuing at 16:42 @16:42:16.93 Gate.power :core,:OFF @16:42:16.94 Gate.power :raw,:OFF @16:42:16.95 Shutdown @16:42:21.54
Powering core bus @16:42:21.72 GoodNews.email "Then, in tube 6: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_121323.rb:14\n{1 => 33,\n 2 => 33,\n 3 => 33,\n 4 => 33,\n 5 => 24,\n 6 => 9,\n 7 => 31}\nCTD data unavailable\nCan@16:42:16, 30% flow, 16.9C, 33% humidity, 15.1psia, 13.754V, 0.260A, 213.845Ah, 3.58W",Subject: "Asleep until 16:42" @16:42:23.22 upsync @16:42:26.84 upsync @16:42:27.27
Gate.power :raw,:ON @16:42:27.95 CS.configure :default @16:42:27.99 PS.configure :default @16:42:28.04 SS.configure :default @16:42:28.09 CC.configure :default @16:42:28.14 PC.configure :default @16:42:28.20 Forearm.configure :loose @16:42:28.25 Elbow.configure :loose @16:42:28.32 SC.configure :loose @16:42:28.37 SE.configure :default @16:42:28.41 CH.configure :default @16:42:28.45 PH.configure :default @16:42:28.48 All dwarf controllers running firmware version 3.85 @16:42:28.59 Valve.reset @16:42:28.86 Intake.select :close @16:42:28.90 Exhaust.select :close @16:42:28.91 CTV.dial :air @16:42:28.98 PSV.dial :air @16:42:29.11
Forearm.home.seek :retract @16:42:29.87 Hand.close @16:42:29.89
Elbow.reconfigure :loose @16:42:30.00 Elbow.jog 1500 @16:42:30.21 PS.home.seek 0ml @16:42:30.28 CSV.dial :waste1 @16:42:30.51
Elbow.reconfigure :loose @16:42:30.55 Elbow.home.seek :clear @16:42:31.01 SSV.dial :spit,avoiding: :exhaust @16:42:31.90 CS.reconfigure :fast @16:42:31.93 SS.home.seek 0ml @16:42:32.02 CS.home.seek 0ml @16:42:33.87
SE.home.seek :down @16:42:34.48 SC.reconfigure :tight @16:42:34.52 SC.home.seek :initial @16:42:38.50 PS.stop @16:43:02.86
SC.configure :limp @16:43:02.91 Forearm.seek :retract @16:43:02.98 Elbow.seek :hammer @16:43:04.01 Forearm.reconfigure :tight @16:43:04.06 Forearm.seek retracted @16:43:04.71 PC.home.seek :open @16:43:04.72
CC.home.seek :open @16:43:08.14 Phase [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] @16:43:08.16 Notice.email "At 16:43:08 began in tube 6: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_121323.rb:14\n{1 => 33,\n 2 => 33,\n 3 => 33,\n 4 => 33,\n 5 => 24,\n 6 => 9,\n 7 => 31}\nCTD data unavailable\nCan@16:43:08, 30% flow, 17.0C, 33% humidity, 15.2psia, 13.730V, 0.315A, 213.850Ah, 4.32W",Subject: "Started DA_WCR_121323 phase initialPurge; da [1000, 1000] #da and wsr are run simultanelously" @16:43:08.18
Starting initialPurge @16:43:08.20 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @16:43:08.27 Hand.open @16:43:08.99 Elbow.reconfigure :tight @16:43:09.04 Elbow.seek FlushPuck::Garage @16:43:10.99 Elbow.reconfigure :loose @16:43:11.09 Forearm.reconfigure :loose @16:43:11.13 Forearm.seek :garage @16:43:12.11 Hand.close @16:43:12.69 upsync @16:43:12.87
Forearm.seek :retract @16:43:13.85 Elbow.reconfigure :tight @16:43:13.90 Elbow.seek CC @16:43:16.88 Elbow.reconfigure :loose @16:43:16.98 Forearm.reconfigure :tight @16:43:17.03 Forearm.seek CC @16:43:18.29 CC.seek :closed @16:43:22.81 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.48 @16:43:22.88 Hand.open @16:43:23.54 Forearm.reconfigure :loose @16:43:23.58 Forearm.seek :retract @16:43:24.58 Flushing the Collection Valve::Manifold @16:43:24.70 CSV.dial CSR @16:43:26.00 Pulling 5ml of CSR.flush @16:43:26.01 CSR.select :flush @16:43:26.13 CS.reconfigure :default @16:43:26.18 CS.seek 5ml @16:43:40.21 delay 5 seconds @16:43:45.22 CSR.select :air @16:43:45.39 CS.reconfigure :fast @16:43:45.44 CS.seek 7.5ml @16:43:52.24 CSV.dial :waste @16:43:53.54 CS.reconfigure :default @16:43:53.59 CS.seek 0ml @16:44:13.64 Purging external Intake @16:44:13.76 CSV.dial :flush @16:44:14.35 CS.reconfigure :fast @16:44:14.40 CS.seek 10ml @16:44:38.96 delay 5 seconds @16:44:43.98 CSV.dial CBV @16:44:44.02
CBV.dial CSV @16:44:44.75 CTV.dial :intake @16:44:45.50 Intake.select :open @16:44:45.61 CS.seek 0ml @16:45:09.27 Intake.select :close @16:45:09.37 CTV.dial :air @16:45:10.18 Flushing upper manifold @16:45:10.30 CSV.dial :flush @16:45:11.04 CS.seek 5ml @16:45:23.83 delay 5 seconds @16:45:28.85 CSV.dial :air @16:45:29.36 CS.seek 7ml @16:45:34.96 CSV.dial CBV @16:45:34.99
CBV.dial CSV @16:45:36.02 CTV.dial :air @16:45:36.10 CS.seek 0ml @16:45:52.77 delay 7 seconds @16:45:59.79 CS.seek 3ml @16:46:07.89 CSV.dial :waste @16:46:08.35 CS.seek 0ml @16:46:16.19 CSV.dial :flush @16:46:16.76 CS.seek 3ml @16:46:24.83 delay 5 seconds @16:46:29.85 CSV.dial :air @16:46:30.37 CS.seek 5ml @16:46:36.08 CSV.dial :air @16:46:36.26 CS.seek 0ml @16:46:48.36 delay 7 seconds @16:46:55.39 CS.seek 3ml @16:47:03.61 CSV.dial :waste @16:47:04.35 CS.seek 0ml @16:47:12.02 Garaging FlushPuck in Collection Clamp @16:47:12.09 Elbow.reconfigure :tight @16:47:12.13 Elbow.seek CC @16:47:12.40 Elbow.reconfigure :loose @16:47:12.50 Forearm.seek CC @16:47:13.53 Hand.close @16:47:14.10 CC.seek :open @16:47:19.13 Forearm.seek :retract @16:47:20.16 Elbow.reconfigure :tight @16:47:20.21 Elbow.seek :garage @16:47:23.17 Elbow.reconfigure :loose @16:47:23.26 Forearm.reconfigure :tight @16:47:23.31 Forearm.seek :garage @16:47:24.33 Hand.open @16:47:25.03 Forearm.reconfigure :loose @16:47:25.08 Forearm.seek :retract @16:47:26.04 da sampling at most 1000ml, wcr at most 1000ml @16:47:26.07 Loading sh1 puck from tube 6 into Collection Clamp @16:47:26.09 DAfans.email "Started da in tube 6 at 16:47:26UTC13-Dec-23\n{1 => 33,\n 2 => 33,\n 3 => 33,\n 4 => 33,\n 5 => 24,\n 6 => 9,\n 7 => 31}\nCTD data unavailable\nCan@16:43:08, 30% flow, 17.0C, 33% humidity, 15.2psia, 13.730V, 0.315A, 213.850Ah, 4.32W",Subject: "da sampling at most 1000ml, wcr at most 1000ml" @16:47:26.35
SC.reconfigure :tight @16:47:26.40 SC.seek 6 @16:47:30.47 upsync @16:47:33.11
SC.configure :limp @16:47:33.34 Elbow.reconfigure :tight @16:47:33.39 Elbow.seek 6 @16:47:35.36 Forearm.reconfigure :tight @16:47:35.41 Forearm.seek 6 + 450 counts @16:47:36.56 SE.reconfigure :pick @16:47:36.62 SE.seek :beamBreak @16:47:39.21 SE.configure :default @16:47:39.37 Elbow.seek 6 @16:47:39.55 Forearm.seek 6 + 300 counts @16:47:39.71 Forearm.reconfigure :loose @16:47:39.75 Hand.close @16:47:40.36 SE.seek :down @16:47:40.57
Forearm.seek :retract @16:47:41.96 Elbow.seek CC @16:47:42.57 SE.stop @16:47:43.59
Elbow.reconfigure :loose @16:47:43.69 Forearm.reconfigure :tight @16:47:43.74 Forearm.seek CC @16:47:45.03 CC.seek :closed @16:47:49.55 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.26 @16:47:49.56 Hand.open @16:47:50.28 Forearm.reconfigure :loose @16:47:50.33 Forearm.seek :retract @16:47:51.33 collectSample 1000ml w/bubble point of 24psi @16:47:51.34 Priming 7ml @16:47:51.35 Intake.select :close @16:47:51.36 Exhaust.select :close @16:47:51.37 SS.hold @16:47:51.58 Calibrating pressure and temperature sensors @16:47:51.61 CTV.dial :air @16:47:51.69 SSV.dial :spit,avoiding: :exhaust @16:47:51.77 SS.reconfigure :fast @16:47:51.81 SS.seek 5ml @16:47:54.71 SSV.dial CBV @16:47:54.75
CBV.dial SSV @16:47:56.07 delay 3.8 seconds @16:48:00.00 Sensor limits: Intake max 99.8psia; Outlet max 93.5psia @16:48:00.01 SSV.dial :spit @16:48:01.33 SS.seek 0ml @16:48:04.38 SSV.dial CBV @16:48:05.73 CTV.dial :intake @16:48:06.52 SSV.dial CBV @16:48:06.55
CBV.dial SSV @16:48:06.73 Allowing 40s for 7.0ml of remaining 7.0ml @16:48:06.74 Intake.select :open @16:48:08.90 Intake @14.6psia, vacuum from 9.1 to 12.1psi @16:48:08.92 SS.reconfigure :inhaling 2.20ml/s @16:48:08.97 SS.seek 7ml @16:48:15.52 Stroke completed -- waiting for pressure to equalize ... @16:48:15.53 SS.reconfigure :exhale @16:48:15.58 SS.hold @16:48:20.85 Intake.select :close @16:48:21.01 SSV.dial :exhaust @16:48:21.79 Exhaust.select :open @16:48:21.91 SS.seek 0ml @16:48:25.99 Exhaust.select :close @16:48:26.11 Sampling 1000.0ml @16:48:26.12 Intake.select :close @16:48:26.13 Exhaust.select :close @16:48:26.14 SS.reconfigure :default @16:48:26.18 SS.hold @16:48:26.38 CTV.dial :intake @16:48:26.45 SSV.dial CBV @16:48:26.48
CBV.dial SSV @16:48:27.25 Allowing 130s for 25.0ml of remaining 1000.0ml @16:48:27.26 Intake.select :open @16:48:29.42 Intake @15.4psia, vacuum from 9.9 to 12.9psi @16:48:29.44 SS.reconfigure :inhaling 2.20ml/s @16:48:29.48 SS.seek 25ml @16:48:44.79 Stroke completed -- waiting for pressure to equalize ... @16:48:44.80 SS.reconfigure :exhale @16:48:44.84 SS.hold @16:49:13.40 Intake.select :close @16:49:13.55 SSV.dial :exhaust @16:49:14.29 Exhaust.select :open @16:49:14.40 SS.seek 0ml @16:49:23.24 Exhaust.select :close @16:49:23.35 SSV.dial CBV @16:49:23.37
CBV.dial SSV @16:49:24.14 Allowing 130s for 25.0ml of remaining 975.0ml @16:49:24.15 Intake.select :open @16:49:26.31 Intake @15.8psia, vacuum from 10.0 to 13.0psi @16:49:26.32 SS.reconfigure :inhaling 2.20ml/s @16:49:26.37 SS.seek 25ml @16:49:36.24 Vacuum too high -- waiting up to 40 seconds @16:49:36.25 SS.reconfigure :exhale @16:49:36.30 SS.hold @16:49:40.20 Intake @17.2psia, vacuum from 10.0 to 13.0psi @16:49:40.21 SS.reconfigure :inhaling 1.76ml/s @16:49:40.26 SS.seek 25ml @16:49:47.05 Stroke completed -- waiting for pressure to equalize ... @16:49:47.06 SS.reconfigure :exhale @16:49:47.12 SS.hold @16:50:11.69 Intake.select :close @16:50:11.84 SSV.dial :exhaust @16:50:12.59 Exhaust.select :open @16:50:12.70 SS.seek 0ml @16:50:21.49 Exhaust.select :close @16:50:21.60 SSV.dial CBV @16:50:21.62
CBV.dial SSV @16:50:22.40 Allowing 130s for 25.0ml of remaining 950.0ml @16:50:22.41 Intake.select :open @16:50:24.58 Intake @16.0psia, vacuum from 10.0 to 13.0psi @16:50:24.59 SS.reconfigure :inhaling 1.76ml/s @16:50:24.64 SS.seek 25ml @16:50:40.76 Vacuum too high -- waiting up to 40 seconds @16:50:40.78 SS.reconfigure :exhale @16:50:40.83 SS.hold @16:50:44.16 Intake @16.6psia, vacuum from 10.0 to 13.0psi @16:50:44.18 SS.reconfigure :inhaling 1.41ml/s @16:50:44.22 SS.seek 25ml @16:50:45.94 Stroke completed -- waiting for pressure to equalize ... @16:50:45.95 SS.reconfigure :exhale @16:50:46.00 SS.hold @16:51:12.50 Intake.select :close @16:51:12.66 SSV.dial :exhaust @16:51:13.42 Exhaust.select :open @16:51:13.54 SS.seek 0ml @16:51:22.32 Exhaust.select :close @16:51:22.84 SSV.dial CBV @16:51:22.87
CBV.dial SSV @16:51:23.65 Allowing 130s for 25.0ml of remaining 925.0ml @16:51:23.66 Intake.select :open @16:51:25.83 Intake @16.1psia, vacuum from 10.0 to 13.0psi @16:51:25.84 SS.reconfigure :inhaling 1.41ml/s @16:51:25.89 SS.seek 25ml @16:51:46.50 Stroke completed -- waiting for pressure to equalize ... @16:51:46.51 SS.reconfigure :exhale @16:51:46.56 SS.hold @16:52:11.07 Intake.select :close @16:52:11.22 SSV.dial :exhaust @16:52:11.97 Exhaust.select :open @16:52:12.09 SS.seek 0ml @16:52:20.91 Exhaust.select :close @16:52:21.02 SSV.dial CBV @16:52:21.04
CBV.dial SSV @16:52:21.82 Allowing 130s for 25.0ml of remaining 900.0ml @16:52:21.83 Intake.select :open @16:52:23.99 Intake @16.2psia, vacuum from 10.0 to 13.0psi @16:52:24.01 SS.reconfigure :inhaling 1.41ml/s @16:52:24.59 SS.seek 25ml @16:52:45.61 Stroke completed -- waiting for pressure to equalize ... @16:52:45.62 SS.reconfigure :exhale @16:52:45.67 SS.hold @16:53:08.12 Intake.select :close @16:53:08.27 SSV.dial :exhaust @16:53:09.02 Exhaust.select :open @16:53:09.13 SS.seek 0ml @16:53:17.86 Exhaust.select :close @16:53:17.97 SSV.dial CBV @16:53:17.99
CBV.dial SSV @16:53:18.76 Allowing 130s for 25.0ml of remaining 875.0ml @16:53:18.77 Intake.select :open @16:53:20.93 Intake @16.1psia, vacuum from 10.0 to 13.0psi @16:53:20.95 SS.reconfigure :inhaling 1.41ml/s @16:53:20.99 SS.seek 25ml @16:53:41.70 Stroke completed -- waiting for pressure to equalize ... @16:53:41.71 SS.reconfigure :exhale @16:53:41.76 SS.hold @16:54:02.47 Intake.select :close @16:54:02.62 SSV.dial :exhaust @16:54:03.37 Exhaust.select :open @16:54:03.48 SS.seek 0ml @16:54:12.25 Exhaust.select :close @16:54:12.36 SSV.dial CBV @16:54:12.38
CBV.dial SSV @16:54:13.16 Allowing 130s for 25.0ml of remaining 850.0ml @16:54:13.17 Intake.select :open @16:54:15.34 Intake @16.3psia, vacuum from 10.0 to 13.0psi @16:54:15.36 SS.reconfigure :inhaling 1.41ml/s @16:54:15.40 SS.seek 25ml @16:54:36.06 Stroke completed -- waiting for pressure to equalize ... @16:54:36.07 SS.reconfigure :exhale @16:54:36.12 SS.hold @16:54:56.92 Intake.select :close @16:54:57.07 SSV.dial :exhaust @16:54:57.84 Exhaust.select :open @16:54:57.95 SS.seek 0ml @16:55:06.89 Exhaust.select :close @16:55:07.00 SSV.dial CBV @16:55:07.02
CBV.dial SSV @16:55:07.80 Allowing 130s for 25.0ml of remaining 825.0ml @16:55:07.81 Intake.select :open @16:55:09.98 Intake @16.2psia, vacuum from 10.0 to 13.0psi @16:55:09.99 SS.reconfigure :inhaling 1.41ml/s @16:55:10.04 SS.seek 25ml @16:55:30.22 Stroke completed -- waiting for pressure to equalize ... @16:55:30.23 SS.reconfigure :exhale @16:55:30.28 SS.hold @16:55:46.90 Intake.select :close @16:55:47.06 SSV.dial :exhaust @16:55:47.83 Exhaust.select :open @16:55:47.94 SS.seek 0ml @16:55:56.73 Exhaust.select :close @16:55:56.84 SSV.dial CBV @16:55:56.86
CBV.dial SSV @16:55:57.64 Allowing 130s for 25.0ml of remaining 800.0ml @16:55:57.65 Intake.select :open @16:55:59.82 Intake @16.3psia, vacuum from 10.0 to 13.0psi @16:55:59.83 SS.reconfigure :inhaling 1.41ml/s @16:55:59.88 SS.seek 25ml @16:56:20.01 Stroke completed -- waiting for pressure to equalize ... @16:56:20.02 SS.reconfigure :exhale @16:56:20.07 SS.hold @16:56:36.83 Intake.select :close @16:56:36.98 SSV.dial :exhaust @16:56:38.09 Exhaust.select :open @16:56:38.21 SS.seek 0ml @16:56:47.14 Exhaust.select :close @16:56:47.25 SSV.dial CBV @16:56:47.27
CBV.dial SSV @16:56:48.05 Allowing 130s for 25.0ml of remaining 775.0ml @16:56:48.06 Intake.select :open @16:56:50.22 Intake @16.3psia, vacuum from 10.0 to 13.0psi @16:56:50.23 SS.reconfigure :inhaling 1.41ml/s @16:56:50.28 SS.seek 25ml @16:57:10.29 Stroke completed -- waiting for pressure to equalize ... @16:57:10.30 SS.reconfigure :exhale @16:57:10.35 SS.hold @16:57:28.68 Intake.select :close @16:57:29.21 SSV.dial :exhaust @16:57:29.97 Exhaust.select :open @16:57:30.08 SS.seek 0ml @16:57:38.75 Exhaust.select :close @16:57:38.86 SSV.dial CBV @16:57:38.88
CBV.dial SSV @16:57:39.66 Allowing 130s for 25.0ml of remaining 750.0ml @16:57:39.67 Intake.select :open @16:57:41.83 Intake @16.2psia, vacuum from 10.0 to 13.0psi @16:57:41.84 SS.reconfigure :inhaling 1.41ml/s @16:57:41.89 SS.seek 25ml @16:58:02.54 Stroke completed -- waiting for pressure to equalize ... @16:58:02.55 SS.reconfigure :exhale @16:58:02.60 SS.hold @16:58:20.99 Intake.select :close @16:58:21.15 SSV.dial :exhaust @16:58:21.90 Exhaust.select :open @16:58:22.02 SS.seek 0ml @16:58:30.75 Exhaust.select :close @16:58:30.85 SSV.dial CBV @16:58:30.87
CBV.dial SSV @16:58:31.64 Allowing 130s for 25.0ml of remaining 725.0ml @16:58:31.65 Intake.select :open @16:58:33.82 Intake @16.2psia, vacuum from 10.0 to 13.0psi @16:58:33.83 SS.reconfigure :inhaling 1.41ml/s @16:58:34.31 SS.seek 25ml @16:58:54.92 Stroke completed -- waiting for pressure to equalize ... @16:58:54.93 SS.reconfigure :exhale @16:58:54.97 SS.hold @16:59:11.26 Intake.select :close @16:59:11.42 SSV.dial :exhaust @16:59:12.18 Exhaust.select :open @16:59:12.30 SS.seek 0ml @16:59:21.07 Exhaust.select :close @16:59:21.18 SSV.dial CBV @16:59:21.21
CBV.dial SSV @16:59:21.98 Allowing 130s for 25.0ml of remaining 700.0ml @16:59:21.99 Intake.select :open @16:59:24.16 Intake @16.3psia, vacuum from 10.0 to 13.0psi @16:59:24.18 SS.reconfigure :inhaling 1.41ml/s @16:59:24.22 SS.seek 25ml @16:59:44.96 Stroke completed -- waiting for pressure to equalize ... @16:59:44.97 SS.reconfigure :exhale @16:59:45.02 SS.hold @17:00:01.55 Intake.select :close @17:00:01.70 SSV.dial :exhaust @17:00:02.46 Exhaust.select :open @17:00:02.58 SS.seek 0ml @17:00:11.48 Exhaust.select :close @17:00:11.59 SSV.dial CBV @17:00:11.61
CBV.dial SSV @17:00:12.39 Allowing 130s for 25.0ml of remaining 675.0ml @17:00:12.40 Intake.select :open @17:00:14.56 Intake @16.2psia, vacuum from 10.0 to 13.0psi @17:00:14.57 SS.reconfigure :inhaling 1.41ml/s @17:00:14.62 SS.seek 25ml @17:00:34.76 Stroke completed -- waiting for pressure to equalize ... @17:00:34.77 SS.reconfigure :exhale @17:00:34.82 SS.hold @17:00:51.43 Intake.select :close @17:00:51.58 SSV.dial :exhaust @17:00:52.34 Exhaust.select :open @17:00:52.45 SS.seek 0ml @17:01:01.40 Exhaust.select :close @17:01:01.51 SSV.dial CBV @17:01:01.53
CBV.dial SSV @17:01:02.31 Allowing 130s for 25.0ml of remaining 650.0ml @17:01:02.32 Intake.select :open @17:01:04.48 Intake @16.3psia, vacuum from 10.0 to 13.0psi @17:01:04.50 SS.reconfigure :inhaling 1.41ml/s @17:01:04.55 SS.seek 25ml @17:01:24.74 Stroke completed -- waiting for pressure to equalize ... @17:01:24.75 SS.reconfigure :exhale @17:01:24.80 SS.hold @17:01:41.42 Intake.select :close @17:01:41.57 SSV.dial :exhaust @17:01:42.34 Exhaust.select :open @17:01:42.45 SS.seek 0ml @17:01:51.16 Exhaust.select :close @17:01:51.27 SSV.dial CBV @17:01:51.29
CBV.dial SSV @17:01:52.07 Allowing 130s for 25.0ml of remaining 625.0ml @17:01:52.08 Intake.select :open @17:01:54.25 Intake @16.3psia, vacuum from 10.0 to 13.0psi @17:01:54.26 SS.reconfigure :inhaling 1.41ml/s @17:01:54.31 SS.seek 25ml @17:02:14.83 Stroke completed -- waiting for pressure to equalize ... @17:02:14.84 SS.reconfigure :exhale @17:02:14.89 SS.hold @17:02:31.16 Intake.select :close @17:02:31.32 SSV.dial :exhaust @17:02:32.07 Exhaust.select :open @17:02:32.18 SS.seek 0ml @17:02:41.59 Exhaust.select :close @17:02:41.69 SSV.dial CBV @17:02:41.71
CBV.dial SSV @17:02:42.49 Allowing 130s for 25.0ml of remaining 600.0ml @17:02:42.51 Intake.select :open @17:02:44.73 Intake @16.2psia, vacuum from 10.0 to 13.0psi @17:02:44.74 SS.reconfigure :inhaling 1.41ml/s @17:02:44.79 SS.seek 25ml @17:03:04.72 Stroke completed -- waiting for pressure to equalize ... @17:03:04.73 SS.reconfigure :exhale @17:03:04.78 SS.hold @17:03:23.11 Intake.select :close @17:03:23.26 SSV.dial :exhaust @17:03:24.02 Exhaust.select :open @17:03:24.14 SS.seek 0ml @17:03:33.12 Exhaust.select :close @17:03:33.22 SSV.dial CBV @17:03:33.24
CBV.dial SSV @17:03:34.01 Allowing 130s for 25.0ml of remaining 575.0ml @17:03:34.02 Intake.select :open @17:03:36.18 Intake @16.2psia, vacuum from 10.0 to 13.0psi @17:03:36.20 SS.reconfigure :inhaling 1.41ml/s @17:03:36.25 SS.seek 25ml @17:03:56.41 Stroke completed -- waiting for pressure to equalize ... @17:03:56.42 SS.reconfigure :exhale @17:03:56.47 SS.hold @17:04:15.08 Intake.select :close @17:04:15.24 SSV.dial :exhaust @17:04:15.99 Exhaust.select :open @17:04:16.11 SS.seek 0ml @17:04:24.85 Exhaust.select :close @17:04:24.96 SSV.dial CBV @17:04:24.98
CBV.dial SSV @17:04:25.76 Allowing 130s for 25.0ml of remaining 550.0ml @17:04:25.77 Intake.select :open @17:04:27.93 Intake @16.4psia, vacuum from 10.0 to 13.0psi @17:04:27.95 SS.reconfigure :inhaling 1.41ml/s @17:04:28.00 SS.seek 25ml @17:04:48.47 Stroke completed -- waiting for pressure to equalize ... @17:04:48.48 SS.reconfigure :exhale @17:04:48.53 SS.hold @17:05:05.60 Intake.select :close @17:05:05.75 SSV.dial :exhaust @17:05:06.51 Exhaust.select :open @17:05:06.61 SS.seek 0ml @17:05:15.38 Exhaust.select :close @17:05:15.49 SSV.dial CBV @17:05:15.51
CBV.dial SSV @17:05:16.29 Allowing 130s for 25.0ml of remaining 525.0ml @17:05:16.30 Intake.select :open @17:05:18.46 Intake @16.4psia, vacuum from 10.0 to 13.0psi @17:05:18.48 SS.reconfigure :inhaling 1.41ml/s @17:05:18.53 SS.seek 25ml @17:05:36.21 Vacuum too high -- waiting up to 40 seconds @17:05:36.23 SS.reconfigure :exhale @17:05:36.28 SS.hold @17:05:43.34 Intake @16.1psia, vacuum from 10.0 to 13.0psi @17:05:43.35 SS.reconfigure :inhaling 1.13ml/s @17:05:43.40 SS.seek 25ml @17:05:47.31 Stroke completed -- waiting for pressure to equalize ... @17:05:47.33 SS.reconfigure :exhale @17:05:47.37 SS.hold @17:06:00.01 Intake.select :close @17:06:00.16 SSV.dial :exhaust @17:06:00.93 Exhaust.select :open @17:06:01.04 SS.seek 0ml @17:06:09.78 Exhaust.select :close @17:06:09.89 SSV.dial CBV @17:06:09.92
CBV.dial SSV @17:06:10.69 Allowing 130s for 25.0ml of remaining 500.0ml @17:06:10.71 Intake.select :open @17:06:12.87 Intake @16.4psia, vacuum from 10.0 to 13.0psi @17:06:12.89 SS.reconfigure :inhaling 1.13ml/s @17:06:12.93 SS.seek 25ml @17:06:37.59 Stroke completed -- waiting for pressure to equalize ... @17:06:37.60 SS.reconfigure :exhale @17:06:37.65 SS.hold @17:06:56.25 Intake.select :close @17:06:56.40 SSV.dial :exhaust @17:06:57.16 Exhaust.select :open @17:06:57.27 SS.seek 0ml @17:07:06.06 Exhaust.select :close @17:07:06.17 SSV.dial CBV @17:07:06.20
CBV.dial SSV @17:07:06.97 Allowing 130s for 25.0ml of remaining 475.0ml @17:07:06.98 Intake.select :open @17:07:09.15 Intake @16.5psia, vacuum from 10.0 to 13.0psi @17:07:09.16 SS.reconfigure :inhaling 1.13ml/s @17:07:09.21 SS.seek 25ml @17:07:33.95 Stroke completed -- waiting for pressure to equalize ... @17:07:33.96 SS.reconfigure :exhale @17:07:34.01 SS.hold @17:07:52.51 Intake.select :close @17:07:52.67 SSV.dial :exhaust @17:07:53.42 Exhaust.select :open @17:07:53.54 SS.seek 0ml @17:08:02.31 Exhaust.select :close @17:08:02.42 SSV.dial CBV @17:08:02.45
CBV.dial SSV @17:08:03.22 Allowing 130s for 25.0ml of remaining 450.0ml @17:08:03.23 Intake.select :open @17:08:05.40 Intake @16.5psia, vacuum from 10.0 to 13.0psi @17:08:05.41 SS.reconfigure :inhaling 1.13ml/s @17:08:05.46 SS.seek 25ml @17:08:27.34 Vacuum too high -- waiting up to 40 seconds @17:08:27.35 SS.reconfigure :exhale @17:08:27.40 SS.hold @17:08:35.41 Intake @16.6psia, vacuum from 10.0 to 13.0psi @17:08:35.43 SS.reconfigure :inhaling 0.91ml/s @17:08:35.47 SS.seek 25ml @17:08:39.19 Stroke completed -- waiting for pressure to equalize ... @17:08:39.20 SS.reconfigure :exhale @17:08:39.24 SS.hold @17:08:51.89 Intake.select :close @17:08:52.04 SSV.dial :exhaust @17:08:52.80 Exhaust.select :open @17:08:52.92 SS.seek 0ml @17:09:01.67 Exhaust.select :close @17:09:01.78 SSV.dial CBV @17:09:01.80
CBV.dial SSV @17:09:02.63 Allowing 130s for 25.0ml of remaining 425.0ml @17:09:02.64 Intake.select :open @17:09:05.22 Intake @16.4psia, vacuum from 10.0 to 13.0psi @17:09:05.24 SS.reconfigure :inhaling 0.91ml/s @17:09:05.29 SS.seek 25ml @17:09:35.19 Stroke completed -- waiting for pressure to equalize ... @17:09:35.20 SS.reconfigure :exhale @17:09:35.24 SS.hold @17:09:55.68 Intake.select :close @17:09:55.83 SSV.dial :exhaust @17:09:56.60 Exhaust.select :open @17:09:56.71 SS.seek 0ml @17:10:05.48 Exhaust.select :close @17:10:05.59 SSV.dial CBV @17:10:05.61
CBV.dial SSV @17:10:06.39 Allowing 130s for 25.0ml of remaining 400.0ml @17:10:06.40 Intake.select :open @17:10:08.57 Intake @16.4psia, vacuum from 10.0 to 13.0psi @17:10:08.58 SS.reconfigure :inhaling 0.91ml/s @17:10:08.63 SS.seek 25ml @17:10:38.54 Stroke completed -- waiting for pressure to equalize ... @17:10:38.55 SS.reconfigure :exhale @17:10:38.60 SS.hold @17:10:59.36 Intake.select :close @17:10:59.51 SSV.dial :exhaust @17:11:00.25 Exhaust.select :open @17:11:00.36 SS.seek 0ml @17:11:09.42 Exhaust.select :close @17:11:09.53 SSV.dial CBV @17:11:09.55
CBV.dial SSV @17:11:10.31 Allowing 130s for 25.0ml of remaining 375.0ml @17:11:10.32 Intake.select :open @17:11:12.49 Intake @16.4psia, vacuum from 10.0 to 13.0psi @17:11:12.50 SS.reconfigure :inhaling 0.91ml/s @17:11:12.55 SS.seek 25ml @17:11:39.36 Vacuum too high -- waiting up to 40 seconds @17:11:39.38 SS.reconfigure :exhale @17:11:39.43 SS.hold @17:11:50.89 Intake @17.5psia, vacuum from 10.0 to 13.0psi @17:11:50.91 SS.reconfigure :inhaling 0.73ml/s @17:11:50.95 SS.seek 25ml @17:11:54.74 Stroke completed -- waiting for pressure to equalize ... @17:11:54.75 SS.reconfigure :exhale @17:11:54.79 SS.hold @17:12:05.91 Intake.select :close @17:12:06.06 SSV.dial :exhaust @17:12:06.81 Exhaust.select :open @17:12:06.93 SS.seek 0ml @17:12:15.87 Exhaust.select :close @17:12:15.98 SSV.dial CBV @17:12:16.00
CBV.dial SSV @17:12:16.76 Allowing 130s for 25.0ml of remaining 350.0ml @17:12:16.78 Intake.select :open @17:12:18.94 Intake @16.5psia, vacuum from 10.0 to 13.0psi @17:12:18.95 SS.reconfigure :inhaling 0.73ml/s @17:12:19.00 SS.seek 25ml @17:12:54.82 Stroke completed -- waiting for pressure to equalize ... @17:12:54.83 SS.reconfigure :exhale @17:12:54.88 SS.hold @17:13:15.24 Intake.select :close @17:13:15.39 SSV.dial :exhaust @17:13:16.56 Exhaust.select :open @17:13:16.67 SS.seek 0ml @17:13:25.45 Exhaust.select :close @17:13:25.56 SSV.dial CBV @17:13:25.58
CBV.dial SSV @17:13:26.36 Allowing 130s for 25.0ml of remaining 325.0ml @17:13:26.37 Intake.select :open @17:13:28.53 Intake @16.5psia, vacuum from 10.0 to 13.0psi @17:13:28.54 SS.reconfigure :inhaling 0.73ml/s @17:13:28.59 SS.seek 25ml @17:14:03.17 Vacuum too high -- waiting up to 40 seconds @17:14:03.19 SS.reconfigure :exhale @17:14:03.24 SS.hold @17:14:13.13 Intake @17.7psia, vacuum from 10.0 to 13.0psi @17:14:13.14 SS.reconfigure :inhaling 0.59ml/s @17:14:13.19 SS.seek 25ml @17:14:15.57 Stroke completed -- waiting for pressure to equalize ... @17:14:15.58 SS.reconfigure :exhale @17:14:15.63 SS.hold @17:14:26.71 Intake.select :close @17:14:26.86 SSV.dial :exhaust @17:14:27.62 Exhaust.select :open @17:14:27.73 SS.seek 0ml @17:14:36.62 Exhaust.select :close @17:14:36.73 SSV.dial CBV @17:14:36.75
CBV.dial SSV @17:14:37.53 Allowing 130s for 25.0ml of remaining 300.0ml @17:14:37.54 Intake.select :open @17:14:39.70 Intake @16.5psia, vacuum from 10.0 to 13.0psi @17:14:39.71 SS.reconfigure :inhaling 0.59ml/s @17:14:39.76 SS.seek 25ml @17:15:24.41 Stroke completed -- waiting for pressure to equalize ... @17:15:24.42 SS.reconfigure :exhale @17:15:24.47 SS.hold @17:15:44.91 Intake.select :close @17:15:45.06 SSV.dial :exhaust @17:15:45.83 Exhaust.select :open @17:15:45.94 SS.seek 0ml @17:15:54.96 Exhaust.select :close @17:15:55.07 SSV.dial CBV @17:15:55.09
CBV.dial SSV @17:15:55.87 Allowing 130s for 25.0ml of remaining 275.0ml @17:15:55.88 Intake.select :open @17:15:58.05 Intake @16.5psia, vacuum from 10.0 to 13.0psi @17:15:58.06 SS.reconfigure :inhaling 0.59ml/s @17:15:58.11 SS.seek 25ml @17:16:42.47 Stroke completed -- waiting for pressure to equalize ... @17:16:42.48 SS.reconfigure :exhale @17:16:42.53 SS.hold @17:17:07.00 Intake.select :close @17:17:07.16 SSV.dial :exhaust @17:17:07.91 Exhaust.select :open @17:17:08.03 SS.seek 0ml @17:17:16.82 Exhaust.select :close @17:17:16.93 SSV.dial CBV @17:17:16.95
CBV.dial SSV @17:17:17.73 Allowing 130s for 25.0ml of remaining 250.0ml @17:17:17.74 Intake.select :open @17:17:19.90 Intake @16.5psia, vacuum from 10.0 to 13.0psi @17:17:19.91 SS.reconfigure :inhaling 0.59ml/s @17:17:19.96 SS.seek 25ml @17:18:01.01 Vacuum too high -- waiting up to 40 seconds @17:18:01.03 SS.reconfigure :exhale @17:18:01.08 SS.hold @17:18:09.73 Intake @17.0psia, vacuum from 10.0 to 13.0psi @17:18:09.74 SS.reconfigure :inhaling 0.47ml/s @17:18:09.79 SS.seek 25ml @17:18:14.99 Stroke completed -- waiting for pressure to equalize ... @17:18:15.00 SS.reconfigure :exhale @17:18:15.04 SS.hold @17:18:29.67 Intake.select :close @17:18:29.82 SSV.dial :exhaust @17:18:30.58 Exhaust.select :open @17:18:30.70 SS.seek 0ml @17:18:39.72 Exhaust.select :close @17:18:39.83 SSV.dial CBV @17:18:39.85
CBV.dial SSV @17:18:40.63 Allowing 130s for 25.0ml of remaining 225.0ml @17:18:40.64 Intake.select :open @17:18:42.81 Intake @16.6psia, vacuum from 10.0 to 13.0psi @17:18:42.82 SS.reconfigure :inhaling 0.47ml/s @17:18:42.87 SS.seek 25ml @17:19:37.81 Stroke completed -- waiting for pressure to equalize ... @17:19:37.82 SS.reconfigure :exhale @17:19:37.87 SS.hold @17:20:00.40 Intake.select :close @17:20:00.55 SSV.dial :exhaust @17:20:01.32 Exhaust.select :open @17:20:01.43 SS.seek 0ml @17:20:10.28 Exhaust.select :close @17:20:10.39 SSV.dial CBV @17:20:10.42
CBV.dial SSV @17:20:11.19 Allowing 130s for 25.0ml of remaining 200.0ml @17:20:11.20 Intake.select :open @17:20:13.37 Intake @16.6psia, vacuum from 10.0 to 13.0psi @17:20:13.39 SS.reconfigure :inhaling 0.47ml/s @17:20:13.43 SS.seek 25ml @17:21:09.14 Stroke completed -- waiting for pressure to equalize ... @17:21:09.15 SS.reconfigure :exhale @17:21:09.20 SS.hold @17:21:33.73 Intake.select :close @17:21:33.88 SSV.dial :exhaust @17:21:34.64 Exhaust.select :open @17:21:34.76 SS.seek 0ml @17:21:43.48 Exhaust.select :close @17:21:43.59 SSV.dial CBV @17:21:43.62
CBV.dial SSV @17:21:44.43 Allowing 130s for 25.0ml of remaining 175.0ml @17:21:44.44 Intake.select :open @17:21:46.60 Intake @16.6psia, vacuum from 10.0 to 13.0psi @17:21:46.61 SS.reconfigure :inhaling 0.47ml/s @17:21:46.66 SS.seek 25ml @17:22:33.44 Vacuum too high -- waiting up to 40 seconds @17:22:33.46 SS.reconfigure :exhale @17:22:33.51 SS.hold @17:22:45.17 Intake @18.8psia, vacuum from 10.0 to 13.0psi @17:22:45.19 SS.reconfigure :inhaling 0.38ml/s @17:22:45.23 SS.seek 25ml @17:22:56.53 Stroke completed -- waiting for pressure to equalize ... @17:22:56.54 SS.reconfigure :exhale @17:22:56.59 SS.hold @17:23:17.30 Intake.select :close @17:23:17.45 SSV.dial :exhaust @17:23:18.22 Exhaust.select :open @17:23:18.33 SS.seek 0ml @17:23:27.14 Exhaust.select :close @17:23:27.25 SSV.dial CBV @17:23:27.27
CBV.dial SSV @17:23:28.05 Allowing 130s for 25.0ml of remaining 150.0ml @17:23:28.06 Intake.select :open @17:23:30.22 Intake @16.4psia, vacuum from 10.0 to 13.0psi @17:23:30.24 SS.reconfigure :inhaling 0.38ml/s @17:23:30.29 SS.seek 25ml @17:24:38.89 Stroke completed -- waiting for pressure to equalize ... @17:24:38.90 SS.reconfigure :exhale @17:24:38.94 SS.hold @17:25:03.34 Intake.select :close @17:25:03.92 SSV.dial :exhaust @17:25:04.69 Exhaust.select :open @17:25:04.80 SS.seek 0ml @17:25:13.61 Exhaust.select :close @17:25:13.71 SSV.dial CBV @17:25:13.74
CBV.dial SSV @17:25:14.51 Allowing 130s for 25.0ml of remaining 125.0ml @17:25:14.53 Intake.select :open @17:25:16.69 Intake @16.6psia, vacuum from 10.0 to 13.0psi @17:25:16.71 SS.reconfigure :inhaling 0.38ml/s @17:25:16.76 SS.seek 25ml @17:26:25.27 Stroke completed -- waiting for pressure to equalize ... @17:26:25.29 SS.reconfigure :exhale @17:26:25.33 SS.hold @17:26:54.34 Intake.select :close @17:26:54.50 SSV.dial :exhaust @17:26:55.26 Exhaust.select :open @17:26:55.38 SS.seek 0ml @17:27:04.40 Exhaust.select :close @17:27:04.51 SSV.dial CBV @17:27:04.54
CBV.dial SSV @17:27:05.31 Allowing 130s for 25.0ml of remaining 100.0ml @17:27:05.32 Intake.select :open @17:27:07.50 Intake @16.7psia, vacuum from 10.0 to 13.0psi @17:27:07.51 SS.reconfigure :inhaling 0.38ml/s @17:27:07.56 SS.seek 25ml @17:28:16.18 Stroke completed -- waiting for pressure to equalize ... @17:28:16.19 SS.reconfigure :exhale @17:28:16.24 SS.hold @17:28:50.96 Intake.select :close @17:28:51.11 SSV.dial :exhaust @17:28:51.87 Exhaust.select :open @17:28:51.98 SS.seek 0ml @17:29:00.85 Exhaust.select :close @17:29:00.96 SSV.dial CBV @17:29:00.99
CBV.dial SSV @17:29:01.76 Allowing 130s for 25.0ml of remaining 75.0ml @17:29:01.77 Intake.select :open @17:29:03.94 Intake @16.6psia, vacuum from 10.0 to 13.0psi @17:29:03.95 SS.reconfigure :inhaling 0.38ml/s @17:29:04.00 SS.seek 25ml @17:30:10.65 Vacuum too high -- waiting up to 40 seconds @17:30:10.67 SS.reconfigure :exhale @17:30:10.72 SS.hold @17:30:29.20 Intake @17.8psia, vacuum from 10.0 to 13.0psi @17:30:29.22 SS.reconfigure :inhaling 0.31ml/s @17:30:29.27 SS.seek 25ml @17:30:32.86 Stroke completed -- waiting for pressure to equalize ... @17:30:32.87 SS.reconfigure :exhale @17:30:32.91 SS.hold @17:30:51.25 Intake.select :close @17:30:51.41 SSV.dial :exhaust @17:30:52.16 Exhaust.select :open @17:30:52.28 SS.seek 0ml @17:31:01.08 Exhaust.select :close @17:31:01.19 SSV.dial CBV @17:31:01.22
CBV.dial SSV @17:31:01.99 Allowing 130s for 25.0ml of remaining 50.0ml @17:31:02.00 Intake.select :open @17:31:04.17 Intake @16.7psia, vacuum from 10.0 to 13.0psi @17:31:04.18 SS.reconfigure :inhaling 0.31ml/s @17:31:04.23 SS.seek 25ml @17:32:33.24 Stroke completed -- waiting for pressure to equalize ... @17:32:33.25 SS.reconfigure :exhale @17:32:33.74 SS.hold @17:33:06.52 Intake.select :close @17:33:06.67 SSV.dial :exhaust @17:33:07.43 Exhaust.select :open @17:33:07.55 SS.seek 0ml @17:33:16.32 Exhaust.select :close @17:33:16.42 SSV.dial CBV @17:33:16.44
CBV.dial SSV @17:33:17.63 Allowing 130s for 25.0ml of remaining 25.0ml @17:33:17.64 Intake.select :open @17:33:19.81 Intake @16.7psia, vacuum from 10.0 to 13.0psi @17:33:19.83 SS.reconfigure :inhaling 0.31ml/s @17:33:19.87 SS.seek 24.98ml @17:34:49.11 Stroke completed -- waiting for pressure to equalize ... @17:34:49.12 SS.reconfigure :exhale @17:34:49.17 SS.hold @17:35:26.34 Intake.select :close @17:35:26.49 SSV.dial :exhaust @17:35:27.25 Exhaust.select :open @17:35:27.37 SS.seek 0ml @17:35:36.42 Exhaust.select :close @17:35:36.53 Sampled 1000.0ml @17:35:36.55 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) Intake.select :close @17:35:36.62 CSV.dial :waste @17:35:36.79 SSV.dial CBV,avoiding: :exhaust @17:35:36.81 CS.seek 0ml @17:35:36.84
CBV.dial SSV @17:35:36.94 CSV.dial :air @17:35:37.68 CS.seek 10ml @17:35:38.72
CTV.dial :air @17:35:39.52 delay 15 seconds @17:35:54.54 SSV.dial :spit,avoiding: :exhaust @17:35:55.87 CTV.dial CSV,avoiding: :intake @17:35:55.90
SS.seek 0ml @17:35:56.01 SSV.dial CBV,avoiding: :exhaust @17:36:02.35 SSV.dial between CBV and plug @17:36:02.83 CBV.dial between SSV and waste2 @17:36:03.21 CSV.dial between air and CTV @17:36:03.54 CS.seek 8.225ml @17:36:08.41 CTV.dial CSV,avoiding: :air @17:36:08.43
CSV.dial CTV @17:36:08.77 CS.reconfigure :pressurize @17:36:08.82 CS.seek 0ml @17:36:22.88 BubblePoint: Intake @24.3psia, Outlet @1.1psia @17:36:22.89 CBV.dial SSV @17:36:23.24 SS.reconfigure :default @17:36:23.28 SS.seek 10ml @17:36:28.83 delay 15 seconds @17:36:43.96 Evacuated: Intake @23.7psia, Outlet @23.3psia @17:36:43.97 SSV.dial :spit,avoiding: :exhaust @17:36:45.01 SS.reconfigure :exhale @17:36:45.05 SS.seek 0ml @17:36:50.00 SS.reconfigure :fast @17:36:50.05 SS.seek 5ml @17:36:52.86 SSV.dial CBV,avoiding: :exhaust @17:36:52.91
CBV.dial SSV @17:36:54.21 CTV.dial :air,avoiding: :intake @17:36:55.55 CSV.dial CTV @17:36:55.58
SS.seek 10ml @17:36:57.12 CSV.dial :air @17:36:57.58 CS.reconfigure :fast @17:36:57.63 CS.seek 0ml @17:36:58.03
SSV.dial :spit,avoiding: :exhaust @17:36:59.35 SS.reconfigure :exhale @17:36:59.40 SS.seek 0ml @17:37:04.42 SS.reconfigure :fast @17:37:04.46 SS.seek 5ml @17:37:05.12 CS.stop @17:37:07.24 SSV.dial CBV,avoiding: :exhaust @17:37:07.26
CBV.dial SSV @17:37:09.60 SSV.dial :spit,avoiding: :exhaust @17:37:10.94 SS.reconfigure :default @17:37:10.99 SS.seek 0ml @17:37:14.26 SS.stop @17:37:14.31 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) Intake.select :close @17:37:14.38 CSV.dial :waste @17:37:14.51 SSV.dial CBV,avoiding: :exhaust @17:37:14.53
CBV.dial SSV @17:37:15.16 CS.seek 0ml @17:37:15.22 CSV.dial :air @17:37:15.89
CTV.dial :air @17:37:15.97 CS.seek 10ml @17:37:16.04
delay 15 seconds @17:37:31.05 SSV.dial :spit,avoiding: :exhaust @17:37:32.37 CTV.dial CSV,avoiding: :intake @17:37:32.41
SS.reconfigure :exhale @17:37:32.49 SS.seek 0ml @17:37:32.87 SSV.dial CBV,avoiding: :exhaust @17:37:40.47 SSV.dial between CBV and plug @17:37:40.96 CBV.dial between SSV and waste2 @17:37:41.35 CSV.dial between air and CTV @17:37:41.65 CS.seek 7.488ml @17:37:48.16 CTV.dial CSV,avoiding: :air @17:37:48.18
CSV.dial CTV @17:37:48.53 CS.reconfigure :pressurize @17:37:48.58 CS.seek 0ml @17:38:04.53 BubblePoint: Intake @24.3psia, Outlet @3.4psia @17:38:04.54 CBV.dial SSV @17:38:04.88 SS.reconfigure :default @17:38:04.92 SS.seek 10ml @17:38:10.54 delay 15 seconds @17:38:25.67 Evacuated: Intake @24.0psia, Outlet @23.7psia @17:38:25.68 SSV.dial :spit,avoiding: :exhaust @17:38:27.16 SS.reconfigure :exhale @17:38:27.21 SS.seek 0ml @17:38:32.20 SS.reconfigure :fast @17:38:32.24 SS.seek 5ml @17:38:35.07 SSV.dial CBV,avoiding: :exhaust @17:38:35.09
CBV.dial SSV @17:38:36.42 CTV.dial :air,avoiding: :intake @17:38:37.76 CSV.dial CTV @17:38:37.82
SS.seek 10ml @17:38:38.99 CSV.dial :air @17:38:39.42 CS.reconfigure :fast @17:38:39.47 CS.seek 0ml @17:38:40.58
SSV.dial :spit,avoiding: :exhaust @17:38:41.91 SS.reconfigure :exhale @17:38:41.96 SS.seek 0ml @17:38:43.74 CS.stop @17:38:47.24
SS.reconfigure :fast @17:38:47.29 SS.seek 5ml @17:38:50.04 SSV.dial CBV,avoiding: :exhaust @17:38:50.06
CBV.dial SSV @17:38:52.40 SSV.dial :spit,avoiding: :exhaust @17:38:53.71 SS.reconfigure :default @17:38:53.75 SS.seek 0ml @17:38:57.03 SS.stop @17:38:57.07 Lysing: Adding 2.0ml AqMeOH @17:38:57.08 CBV.dial between CSV and waste1,avoiding: :DBV @17:38:58.57 CTV.dial CSV @17:38:59.90 CSV.dial CSR @17:39:00.69 Pulling 1ml of CSR.AqMeOH @17:39:00.70 CSR.select :AqMeOH @17:39:00.81 CS.reconfigure :default @17:39:00.86 CS.seek 1ml @17:39:04.34 delay 5 seconds @17:39:09.36 CSR.select :air @17:39:09.56 CS.seek 6ml @17:39:23.21 delay 2 seconds @17:39:25.23 CSV.dial :waste2 @17:39:25.80 CS.reconfigure :fast @17:39:25.84 CS.seek 0ml @17:39:40.31 CSV.dial CSR @17:39:40.76 Pulling 2ml of CSR.AqMeOH @17:39:40.77 CSR.select :AqMeOH @17:39:40.89 CS.reconfigure :default @17:39:40.93 CS.seek 2ml @17:39:46.93 delay 5 seconds @17:39:51.95 CSR.select :air @17:39:52.12 CS.seek 5ml @17:40:00.59 delay 2 seconds @17:40:02.61 CSV.dial CTV @17:40:03.05 CS.seek 1ml @17:40:14.73 Before lysing Collection Puck Heater at 13.9C/28.9psia @17:40:14.75 Heating Puck.from 6, :sh1 in Collection Clamp to 70C for 10:00 @17:40:14.77 CH.seek 70C,8:00 @17:42:27.31 CH.hold 10:00,70C @17:42:27.51 Begun lysing Collection Puck Heater at 68.1C/35.7psia @17:52:27.51 Ended lysing Collection Puck Heater at 70C/34.6psia @17:52:27.53 CS.seek 0ml @17:52:30.89 CTV.dial between spare and air,avoiding: :intake @17:52:31.92 CSV.dial :air @17:52:32.36 CS.seek 5ml @17:52:45.95 CSV.dial CTV @17:52:46.41 delay 2 seconds @17:52:48.42 CSV.dial :air @17:52:49.00 delay 2 seconds @17:52:51.02 CSV.dial CTV @17:52:51.45 delay 2 seconds @17:52:53.47 CSV.dial :air @17:52:53.92 delay 2 seconds @17:52:55.94 CS.seek 0ml @17:53:09.69 Puck @72.6psia after final lysis pressurization @17:53:09.71 Recovering Lysate into Collection syringe CSV.dial CBV @17:53:10.72 CBV.dial CSV @17:53:11.10 CS.seek 4.999ml @17:53:24.70 delay 5 seconds @17:53:29.72 CTV.dial :air @17:53:30.24 CS.seek 7ml @17:53:36.17 delay 5 seconds @17:53:41.19 CSV.dial :air2 @17:53:41.63 CS.seek 10ml @17:53:50.11 CSV.dial between air and CTV,avoiding: CBV @17:53:51.27 Lysing Complete @17:53:51.34 Storing sh1 puck in Collection Clamp to tube 5 @17:53:51.51 SC.reconfigure :tight @17:53:51.55 SC.seek 5 @17:53:55.12 SC.configure :limp @17:53:55.76 Elbow.reconfigure :tight @17:53:55.82 Elbow.seek 5 @17:53:57.57 Forearm.reconfigure :tight @17:53:57.62 Forearm.seek 5 + 450 counts @17:53:58.63 SE.reconfigure :pick @17:53:58.69 SE.seek :beamBreak @17:54:00.31 SE.configure :default @17:54:00.47 Elbow.reconfigure :limp @17:54:00.52 Forearm.reconfigure :loose @17:54:00.57 Forearm.seek 5 @17:54:00.84 Elbow.hold @17:54:00.90 Elbow.reconfigure :tight @17:54:01.00 SE.seek 23.14 Pucks below Top Plate @17:54:01.52 Forearm.force -20 @17:54:01.57 Forearm.seek 5 + 306 counts @17:54:01.84 Forearm.reconfigure :tight @17:54:01.89 Forearm.seek 5 + 450 counts @17:54:02.08 SE.seek 24.14 Pucks below Top Plate @17:54:02.64 Forearm.reconfigure :loose @17:54:02.69 Forearm.seek :retract @17:54:03.62 Elbow.seek CC @17:54:05.33 Elbow.reconfigure :loose @17:54:05.42 Forearm.seek CC @17:54:06.40 Hand.close @17:54:07.05 CC.seek :open @17:54:11.77 Forearm.seek :retract @17:54:12.73 Elbow.reconfigure :tight @17:54:12.78 Elbow.seek 5 @17:54:14.53 Elbow.reconfigure :limp @17:54:14.58 Forearm.seek 5 @17:54:16.04 Elbow.hold @17:54:16.11 Elbow.reconfigure :tight @17:54:16.16 Hand.open @17:54:16.92 Forearm.force -20 @17:54:16.96 Forearm.seek 5 + 316 counts @17:54:17.24 Forearm.reconfigure :tight @17:54:17.29 Forearm.seek 5 + 450 counts @17:54:17.55 SE.seek :down @17:54:18.65 SE.stop @17:54:18.76
Forearm.reconfigure :loose @17:54:18.81 Forearm.seek :retract @17:54:19.75 Tranferring DA @17:54:19.76 Beginning samplePassC @17:54:19.77 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @17:54:19.96 Elbow.seek FlushPuck::Garage @17:54:21.82 Elbow.reconfigure :loose @17:54:21.92 Forearm.seek :garage @17:54:22.93 Hand.close @17:54:23.66 Forearm.seek :retract @17:54:24.61 Elbow.reconfigure :tight @17:54:24.65 Elbow.seek CC @17:54:27.57 Elbow.reconfigure :loose @17:54:27.67 Forearm.reconfigure :tight @17:54:27.71 Forearm.seek CC @17:54:29.03 CC.seek :closed @17:54:33.55 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.36 @17:54:33.62 Hand.open @17:54:34.23 Forearm.reconfigure :loose @17:54:34.28 Forearm.seek :retract @17:54:35.24 PSV.dial PSR @17:54:36.62 Pulling 1.5ml of PSR.flush @17:54:36.63 PSR.select :flush @17:54:36.75 PS.seek 1.5ml @17:54:52.92 delay 5 seconds @17:54:57.94 PSV.dial :waste1 @17:54:58.41 PS.seek 0ml @17:55:14.51 PSV.dial PSR @17:55:14.98 PSR.select :air @17:55:15.10 PS.seek 2.5ml @17:55:41.49 PRV.dial CBV,avoiding: :ab2 @17:55:41.51
CBV.dial PRV @17:55:43.25 PSV.dial :mixloop @17:55:44.28 CTV.dial :air,avoiding: :intake @17:55:44.35 PTV.dial :PRVmixing @17:55:45.69 PS.seek 0ml @17:56:12.08 PSV.dial :air @17:56:12.55 PS.seek 2.5ml @17:56:38.92 PSV.dial :mixloop @17:56:39.40 PS.seek 0ml @17:57:05.73 PSV.dial :air @17:57:06.33 PS.seek 2.5ml @17:57:32.67 PSV.dial :mixloop @17:57:33.13 PS.seek 0ml @17:57:59.64 Garaging FlushPuck in Collection Clamp @17:57:59.71 Elbow.reconfigure :tight @17:57:59.76 Elbow.seek CC @17:58:00.02 Elbow.reconfigure :loose @17:58:00.11 Forearm.seek CC @17:58:01.12 Hand.close @17:58:01.67 CC.seek :open @17:58:06.35 Forearm.seek :retract @17:58:07.36 Elbow.reconfigure :tight @17:58:07.40 Elbow.seek :garage @17:58:10.38 Elbow.reconfigure :loose @17:58:10.48 Forearm.reconfigure :tight @17:58:10.53 Forearm.seek :garage @17:58:11.59 Hand.open @17:58:12.23 Forearm.reconfigure :loose @17:58:12.27 Forearm.seek :retract @17:58:13.27 PSV.dial :mixloop @17:58:13.34 PTV.dial :PRVmixing @17:58:13.42 PRV.dial CBV @17:58:13.44
CBV.dial PRV @17:58:13.53 CTV.dial :air,avoiding: :intake @17:58:13.61 Loading lyfil puck from tube 6 into Collection Clamp @17:58:13.84 SC.reconfigure :tight @17:58:13.89 SC.seek 6 @17:58:17.43 SC.configure :limp @17:58:17.65 Elbow.reconfigure :tight @17:58:17.70 Elbow.seek 6 @17:58:19.64 Forearm.reconfigure :tight @17:58:19.69 Forearm.seek 6 + 450 counts @17:58:20.71 SE.reconfigure :pick @17:58:20.77 SE.seek :beamBreak @17:58:23.38 SE.configure :default @17:58:23.54 Elbow.seek 6 @17:58:23.72 Forearm.seek 6 + 300 counts @17:58:23.95 Forearm.reconfigure :loose @17:58:23.99 Hand.close @17:58:24.71 SE.seek :down @17:58:24.91
Forearm.seek :retract @17:58:25.84 Elbow.seek CC @17:58:27.02 SE.stop @17:58:27.46
Elbow.reconfigure :loose @17:58:27.56 Forearm.reconfigure :tight @17:58:27.61 Forearm.seek CC @17:58:28.88 CC.seek :closed @17:58:33.41 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.14 @17:58:33.42 Hand.open @17:58:34.10 Forearm.reconfigure :loose @17:58:34.15 Forearm.seek :retract @17:58:35.14 CTV.dial CSV,avoiding: :intake @17:58:36.49 CSV.dial CTV @17:58:36.94 PS.seek 2.5ml @17:59:03.32 CS.seek 4.5ml @17:59:18.16 delay 8 seconds @17:59:26.19 PSV.dial :air @17:59:26.65 delay 2 seconds @17:59:28.67 PSV.dial :waste2 @17:59:29.41 PS.seek 0ml @17:59:55.73 CBV.dial between PRV and waste1 @17:59:56.05 CSV.dial :air @17:59:56.50 CS.seek 10ml @18:00:11.47 CSV.dial CTV @18:00:11.94 CS.seek 0ml @18:00:38.57 CBV.dial :waste1 @18:00:38.88 delay 5 seconds @18:00:43.90 CTV.dial :air,avoiding: :intake @18:00:45.75 Storing lyfil puck in Collection Clamp to tube 5 @18:00:45.93 SC.reconfigure :tight @18:00:45.98 SC.seek 5 @18:00:49.52 SC.configure :limp @18:00:49.74 Elbow.reconfigure :tight @18:00:49.79 Elbow.seek 5 @18:00:51.55 Forearm.reconfigure :tight @18:00:51.60 Forearm.seek 5 + 450 counts @18:00:52.63 SE.reconfigure :pick @18:00:52.69 SE.seek :beamBreak @18:00:54.16 SE.configure :default @18:00:54.32 Elbow.reconfigure :limp @18:00:54.37 Forearm.reconfigure :loose @18:00:54.42 Forearm.seek 5 @18:00:54.64 Elbow.hold @18:00:54.70 Elbow.reconfigure :tight @18:00:54.80 SE.seek 24.14 Pucks below Top Plate @18:00:55.38 Forearm.force -20 @18:00:55.42 Forearm.seek 5 + 311 counts @18:00:55.70 Forearm.reconfigure :tight @18:00:55.75 Forearm.seek 5 + 450 counts @18:00:55.93 SE.seek 25.14 Pucks below Top Plate @18:00:56.51 Forearm.reconfigure :loose @18:00:56.56 Forearm.seek :retract @18:00:57.48 Elbow.seek CC @18:00:59.11 Elbow.reconfigure :loose @18:00:59.21 Forearm.seek CC @18:01:00.61 Hand.close @18:01:01.29 CC.seek :open @18:01:06.03 Forearm.seek :retract @18:01:07.01 Elbow.reconfigure :tight @18:01:07.06 Elbow.seek 5 @18:01:08.83 Elbow.reconfigure :limp @18:01:08.88 Forearm.seek 5 @18:01:09.93 Elbow.hold @18:01:09.99 Elbow.reconfigure :tight @18:01:10.04 Hand.open @18:01:10.78 Forearm.force -20 @18:01:10.83 Forearm.seek 5 + 304 counts @18:01:11.11 Forearm.reconfigure :tight @18:01:11.16 Forearm.seek 5 + 450 counts @18:01:11.42 SE.seek :down @18:01:12.42 SE.stop @18:01:12.58
Forearm.reconfigure :loose @18:01:12.62 Forearm.seek :retract @18:01:13.55 PSV.dial :air @18:01:14.31 PS.seek 2.5ml @18:01:40.70 delay 1 second @18:01:41.71 PSV.dial :mixloop @18:01:42.17 delay 1 second @18:01:43.18 PSV.dial :air @18:01:43.65 delay 1 second @18:01:44.67 PSV.dial :mixloop @18:01:45.14 delay 1 second @18:01:46.15 PSV.dial :air @18:01:46.62 delay 1 second @18:01:47.64 PS.seek 0ml @18:02:14.01 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @18:02:14.61 Elbow.seek FlushPuck::Garage @18:02:16.45 Elbow.reconfigure :loose @18:02:16.55 Forearm.seek :garage @18:02:17.55 Hand.close @18:02:18.28 Forearm.seek :retract @18:02:19.24 Elbow.reconfigure :tight @18:02:19.29 Elbow.seek PC @18:02:20.56 Elbow.reconfigure :loose @18:02:20.66 Forearm.reconfigure :tight @18:02:20.71 Forearm.seek PC @18:02:21.94 PC.seek :closed @18:02:26.60 Processing Clamp onSpring/offSpring for 16/26 samples, on/off torque 1.62 @18:02:26.67 Hand.open @18:02:27.35 Forearm.reconfigure :loose @18:02:27.39 Forearm.seek :retract @18:02:28.35 PRV.dial :air @18:02:28.82 PSV.dial :mixloop @18:02:29.36 PS.seek 0.3ml @18:02:33.41 PSV.dial PSR @18:02:34.47 Pulling 0.4ml of PSR.air @18:02:34.48 PSR.select :air @18:02:34.50 PS.seek 0.7ml @18:02:39.35 delay 3 seconds @18:02:42.37 PSR.select :air @18:02:42.39 PS.seek 2.5ml @18:03:01.54 delay 3 seconds @18:03:04.56 PSV.dial :mixloop @18:03:05.60 PTV.dial :mixing @18:03:06.35 PBV.dial :waste2 @18:03:07.77 PS.seek 0ml @18:03:34.20 Garaging FlushPuck in Processing Clamp @18:03:34.27 Elbow.reconfigure :tight @18:03:34.31 Elbow.seek PC @18:03:34.44 Elbow.reconfigure :loose @18:03:34.54 Forearm.seek PC @18:03:35.52 Hand.close @18:03:36.16 PC.seek :open @18:03:40.87 Forearm.seek :retract @18:03:41.83 Elbow.reconfigure :tight @18:03:41.88 Elbow.seek :garage @18:03:43.25 Elbow.reconfigure :loose @18:03:43.35 Forearm.reconfigure :tight @18:03:43.39 Forearm.seek :garage @18:03:44.46 Hand.open @18:03:45.10 Forearm.reconfigure :loose @18:03:45.14 Forearm.seek :retract @18:03:46.13 Loading da puck from tube 6 into Processing Clamp @18:03:46.37 SC.reconfigure :tight @18:03:46.41 SC.seek 6 @18:03:50.00 SC.configure :limp @18:03:50.21 Elbow.reconfigure :tight @18:03:50.26 Elbow.seek 6 @18:03:52.19 Forearm.reconfigure :tight @18:03:52.23 Forearm.seek 6 + 450 counts @18:03:53.25 SE.reconfigure :pick @18:03:53.31 SE.seek :beamBreak @18:03:56.02 SE.configure :default @18:03:56.18 Elbow.seek 6 @18:03:56.80 Forearm.seek 6 + 300 counts @18:03:57.00 Forearm.reconfigure :loose @18:03:57.05 Hand.close @18:03:57.68 SE.seek :down @18:03:57.88
Forearm.seek :retract @18:03:58.87 Elbow.seek PC @18:04:00.08 SE.stop @18:04:01.39
Elbow.reconfigure :loose @18:04:01.49 Forearm.reconfigure :tight @18:04:01.54 Forearm.seek PC @18:04:02.87 PC.seek :closed @18:04:07.52 Processing Clamp onSpring/offSpring for 16/26 samples, on/off torque 1.63 @18:04:07.53 Hand.open @18:04:08.22 Forearm.reconfigure :loose @18:04:08.27 Forearm.seek :retract @18:04:09.26 Heating Puck.from 6, :da in Processing Clamp to 28C for 2:00:00 @18:04:09.28 PH.seek 28C,5:00 @18:04:50.91 PH.hold 2:00:00,28C @18:04:50.95 delay 1:30 @18:06:20.99 PSR.select :air @18:06:21.00 PSV.dial PSR @18:06:22.05 Pulling 0.5ml of PSR.wash @18:06:22.06 PSR.select :wash @18:06:22.18 PS.seek 0.5ml @18:06:28.32 delay 5 seconds @18:06:33.33 PSR.select :air @18:06:33.46 PS.seek 2ml @18:06:49.56 PTV.dial :puck @18:06:50.32 PSV.dial PTV @18:06:51.08 PBV.dial :waste1 @18:06:52.47 PS.seek 1.5ml @18:06:58.38 delay 3 seconds @18:07:01.40 PBV.dial between PSV and waste1 @18:07:01.74 PS.seek 0ml @18:07:17.91 delay 5:00 @18:12:17.94 recoverPSRreagent08 @18:12:17.95 PTV.dial :puck @18:12:18.01 PBV.dial :waste1 @18:12:18.37 delay 30 seconds @18:12:48.40 PBV.dial between PSV and waste1 @18:12:48.76 PSR.select :air @18:12:48.78 PSV.dial PSR @18:12:49.66 PS.reconfigure :fast @18:12:49.70 PS.seek 2.5ml @18:13:13.40 PSV.dial PTV @18:13:14.14 delay 5 seconds @18:13:19.16 PSV.dial PSR @18:13:19.90 delay 5 seconds @18:13:24.92 PSV.dial PTV @18:13:25.68 delay 5 seconds @18:13:30.70 PSV.dial :air @18:13:31.45 PS.seek 0ml @18:13:55.25 PTV.dial :PRVmixing,via: PRV @18:13:56.56 PSV.dial :mixloop @18:13:57.03 PRV.dial :ab1 @18:13:57.78 PS.reconfigure :slow @18:13:57.83 PS.seek 0.15ml @18:14:01.59 delay 2 seconds @18:14:03.61 CBV.dial PRV @18:14:03.63
PRV.dial CBV @18:14:04.21 PS.seek 0.35ml @18:14:08.74 delay 2 seconds @18:14:10.76 CBV.dial between PRV and PSV @18:14:11.07 PRV.dial :air @18:14:11.53 PS.seek 0.9ml @18:14:23.24 PRV.dial :waste2 @18:14:24.00 PS.seek 0.5ml @18:14:32.65 addPMLReagent08 @18:14:32.66 PSV.dial :air @18:14:33.12 PS.reconfigure :default @18:14:33.17 PS.seek 2ml @18:14:49.37 PSV.dial :PML @18:14:49.84 PTV.dial :mixing @18:14:50.59 PBV.dial :waste1 @18:14:51.02 PS.seek 1.85ml @18:14:53.40 PBV.dial between PSV and waste1 @18:14:53.78 PS.seek 0ml @18:15:13.46 delay 2 seconds @18:15:15.48 Beginning samplePassC @18:15:15.49 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @18:15:15.68 Elbow.reconfigure :tight @18:15:15.72 Elbow.seek FlushPuck::Garage @18:15:17.13 Elbow.reconfigure :loose @18:15:17.23 Forearm.seek :garage @18:15:18.17 Hand.close @18:15:18.90 Forearm.seek :retract @18:15:19.87 Elbow.reconfigure :tight @18:15:19.92 Elbow.seek CC @18:15:22.86 Elbow.reconfigure :loose @18:15:22.96 Forearm.reconfigure :tight @18:15:23.44 Forearm.seek CC @18:15:24.67 CC.seek :closed @18:15:29.19 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.50 @18:15:29.26 Hand.open @18:15:29.96 Forearm.reconfigure :loose @18:15:30.01 Forearm.seek :retract @18:15:30.96 PSV.dial PSR @18:15:32.02 Pulling 1.5ml of PSR.flush @18:15:32.03 PSR.select :flush @18:15:32.16 PS.seek 1.5ml @18:15:48.24 delay 5 seconds @18:15:53.26 PSV.dial :waste1 @18:15:53.73 PS.seek 0ml @18:16:09.87 PSV.dial PSR @18:16:10.34 PSR.select :air @18:16:10.45 PS.seek 2.5ml @18:16:36.78 PRV.dial CBV,avoiding: :ab2 @18:16:36.80
CBV.dial PRV @18:16:38.43 PSV.dial :mixloop @18:16:39.48 CTV.dial :air,avoiding: :intake @18:16:39.55 PTV.dial :PRVmixing @18:16:40.33 PS.seek 0ml @18:17:06.74 PSV.dial :air @18:17:07.22 PS.seek 2.5ml @18:17:33.65 PSV.dial :mixloop @18:17:34.14 PS.seek 0ml @18:18:00.55 PSV.dial :air @18:18:01.03 PS.seek 2.5ml @18:18:27.43 PSV.dial :mixloop @18:18:27.90 PS.seek 0ml @18:18:54.18 Garaging FlushPuck in Collection Clamp @18:18:54.70 Elbow.reconfigure :tight @18:18:54.75 Elbow.seek CC @18:18:55.07 Elbow.reconfigure :loose @18:18:55.17 Forearm.seek CC @18:18:56.21 Hand.close @18:18:56.78 CC.seek :open @18:19:01.39 Forearm.seek :retract @18:19:02.37 Elbow.reconfigure :tight @18:19:02.42 Elbow.seek :garage @18:19:05.41 Elbow.reconfigure :loose @18:19:05.51 Forearm.reconfigure :tight @18:19:05.55 Forearm.seek :garage @18:19:06.62 Hand.open @18:19:07.33 Forearm.reconfigure :loose @18:19:07.38 Forearm.seek :retract @18:19:08.40 delayUntil "18:23:15.47" @18:19:08.43 Whole Cell Archive sampling 1000ml @18:19:08.45 Loading wcr puck from tube 6 into Collection Clamp @18:19:08.69 SC.reconfigure :tight @18:19:08.74 SC.seek 6 @18:19:08.83 SC.configure :limp @18:19:09.05 Elbow.reconfigure :tight @18:19:09.10 Elbow.seek 6 @18:19:10.99 Forearm.reconfigure :tight @18:19:11.04 Forearm.seek 6 + 450 counts @18:19:12.09 SE.reconfigure :pick @18:19:12.15 SE.seek :beamBreak @18:19:14.87 SE.configure :default @18:19:15.45 Elbow.seek 6 @18:19:15.65 Forearm.seek 6 + 300 counts @18:19:15.87 Forearm.reconfigure :loose @18:19:15.92 Hand.close @18:19:16.54 SE.seek :down @18:19:16.75 Forearm.seek :retract @18:19:17.69 Elbow.seek CC @18:19:19.02 SE.stop @18:19:19.35 Elbow.reconfigure :loose @18:19:19.46 Forearm.reconfigure :tight @18:19:19.51 Forearm.seek CC @18:19:20.74 CC.seek :closed @18:19:25.28 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.25 @18:19:25.29 Hand.open @18:19:25.96 Forearm.reconfigure :loose @18:19:26.01 Forearm.seek :retract @18:19:27.06 collectSample 1000ml w/bubble point of 35psi @18:19:27.07 Priming 7ml @18:19:27.08 Intake.select :close @18:19:27.09 Exhaust.select :close @18:19:27.10 SS.hold @18:19:27.31 Calibrating pressure sensors @18:19:27.32 CTV.dial :air @18:19:27.34 SSV.dial :spit,avoiding: :exhaust @18:19:27.43 SS.reconfigure :fast @18:19:27.48 SS.seek 5ml @18:19:30.25 SSV.dial CBV @18:19:30.27 CBV.dial SSV @18:19:32.09 delay 3.8 seconds @18:19:36.03 Sensor limits: Intake max 100.6psia; Outlet max 92.9psia @18:19:36.04 SSV.dial :spit @18:19:37.38 SS.seek 0ml @18:19:40.04 SSV.dial CBV @18:19:41.36 CTV.dial :intake @18:19:42.13 SSV.dial CBV @18:19:42.15 CBV.dial SSV @18:19:42.30 Allowing 40s for 7.0ml of remaining 7.0ml @18:19:42.32 Intake.select :open @18:19:44.48 Intake @14.6psia, vacuum from 9.1 to 12.1psi @18:19:44.50 SS.reconfigure :inhaling 2.20ml/s @18:19:44.55 SS.seek 7ml @18:19:51.43 Stroke completed -- waiting for pressure to equalize ... @18:19:51.44 SS.reconfigure :exhale @18:19:51.49 SS.hold @18:20:11.95 Intake.select :close @18:20:12.11 SSV.dial :exhaust @18:20:12.89 Exhaust.select :open @18:20:13.00 SS.seek 0ml @18:20:17.34 Exhaust.select :close @18:20:17.45 Sampling 1000.0ml @18:20:17.47 Intake.select :close @18:20:17.48 Exhaust.select :close @18:20:17.49 SS.reconfigure :default @18:20:17.54 SS.hold @18:20:17.74 CTV.dial :intake @18:20:17.98 SSV.dial CBV @18:20:18.01 CBV.dial SSV @18:20:18.79 Allowing 130s for 25.0ml of remaining 1000.0ml @18:20:18.81 Intake.select :open @18:20:20.97 Intake @15.4psia, vacuum from 9.9 to 12.9psi @18:20:20.99 SS.reconfigure :inhaling 2.20ml/s @18:20:21.03 SS.seek 25ml @18:20:28.85 Vacuum too high -- waiting up to 40 seconds @18:20:28.86 SS.reconfigure :exhale @18:20:28.92 SS.hold @18:20:38.22 Intake @20.0psia, vacuum from 10.0 to 13.0psi @18:20:38.23 SS.reconfigure :inhaling 1.76ml/s @18:20:38.28 SS.seek 25ml @18:20:40.50 Vacuum too high -- waiting up to 40 seconds @18:20:40.52 SS.reconfigure :exhale @18:20:40.57 SS.hold @18:20:42.79 Intake @21.0psia, vacuum from 10.0 to 13.0psi @18:20:42.81 SS.reconfigure :inhaling 1.41ml/s @18:20:42.86 SS.seek 25ml @18:20:44.47 Vacuum too high -- waiting up to 40 seconds @18:20:44.49 SS.reconfigure :exhale @18:20:44.54 SS.hold @18:20:46.30 Intake @20.9psia, vacuum from 10.0 to 13.0psi @18:20:46.32 SS.reconfigure :inhaling 1.13ml/s @18:20:46.36 SS.seek 25ml @18:20:48.07 Vacuum too high -- waiting up to 40 seconds @18:20:48.08 SS.reconfigure :exhale @18:20:48.13 SS.hold @18:20:49.76 Intake @20.7psia, vacuum from 10.0 to 13.0psi @18:20:49.78 SS.reconfigure :inhaling 0.91ml/s @18:20:49.83 SS.seek 25ml @18:20:51.94 Vacuum too high -- waiting up to 40 seconds @18:20:51.96 SS.reconfigure :exhale @18:20:52.01 SS.hold @18:20:53.72 Intake @20.6psia, vacuum from 10.0 to 13.0psi @18:20:53.74 SS.reconfigure :inhaling 0.73ml/s @18:20:53.79 SS.seek 25ml @18:20:56.85 Vacuum too high -- waiting up to 40 seconds @18:20:56.86 SS.reconfigure :exhale @18:20:56.92 SS.hold @18:20:58.64 Intake @20.5psia, vacuum from 10.0 to 13.0psi @18:20:58.66 SS.reconfigure :inhaling 0.59ml/s @18:20:58.71 SS.seek 25ml @18:21:05.56 Vacuum too high -- waiting up to 40 seconds @18:21:05.58 SS.reconfigure :exhale @18:21:05.63 SS.hold @18:21:07.33 Intake @20.6psia, vacuum from 10.0 to 13.0psi @18:21:07.79 SS.reconfigure :inhaling 0.47ml/s @18:21:07.85 SS.seek 25ml @18:21:12.20 Stroke completed -- waiting for pressure to equalize ... @18:21:12.21 SS.reconfigure :exhale @18:21:12.26 SS.hold @18:21:43.01 Intake.select :close @18:21:43.17 SSV.dial :exhaust @18:21:43.93 Exhaust.select :open @18:21:44.05 SS.seek 0ml @18:21:52.87 Exhaust.select :close @18:21:52.98 SSV.dial CBV @18:21:53.01 CBV.dial SSV @18:21:53.79 Allowing 130s for 25.0ml of remaining 975.0ml @18:21:53.80 Intake.select :open @18:21:55.97 Intake @15.9psia, vacuum from 10.0 to 13.0psi @18:21:55.99 SS.reconfigure :inhaling 0.47ml/s @18:21:56.04 SS.seek 25ml @18:22:51.90 Stroke completed -- waiting for pressure to equalize ... @18:22:51.91 SS.reconfigure :exhale @18:22:51.96 SS.hold @18:23:15.48 recoverPRVreagent08 @18:23:15.49 PTV.dial :mixing @18:23:16.25 PBV.dial :waste1 @18:23:16.63 delay 30 seconds @18:23:21.03 Intake.select :close @18:23:21.19 SSV.dial :exhaust @18:23:21.96 Exhaust.select :open @18:23:22.08 SS.seek 0ml @18:23:31.13 Exhaust.select :close @18:23:31.24 SSV.dial CBV @18:23:31.27 CBV.dial SSV @18:23:32.05 Allowing 130s for 25.0ml of remaining 950.0ml @18:23:32.07 Intake.select :open @18:23:34.23 Intake @16.1psia, vacuum from 10.0 to 13.0psi @18:23:34.25 SS.reconfigure :inhaling 0.47ml/s @18:23:34.30 SS.seek 25ml @18:23:46.65 PBV.dial between PSV and waste1 @18:23:47.03 PSV.dial :air @18:23:47.50 PS.reconfigure :fast @18:23:47.55 PS.seek 2.5ml @18:24:11.36 PSV.dial :mixloop @18:24:11.83 delay 2 seconds @18:24:13.85 PSV.dial :air @18:24:14.31 delay 2 seconds @18:24:16.33 PSV.dial :mixloop @18:24:16.80 delay 2 seconds @18:24:18.81 PSV.dial :air @18:24:19.41 PS.seek 0ml @18:24:29.46 Stroke completed -- waiting for pressure to equalize ... @18:24:29.47 SS.reconfigure :exhale @18:24:29.52 SS.hold @18:24:42.76 washCycleDA #1 of 2 @18:24:42.77 PSR.select :air @18:24:42.79 PSV.dial PSR @18:24:44.17 Pulling 0.5ml of PSR.wash @18:24:44.18 PSR.select :wash @18:24:44.31 PS.reconfigure :default @18:24:44.36 PS.seek 0.5ml @18:24:50.29 delay 5 seconds @18:24:55.31 PSR.select :air @18:24:55.42 PS.seek 2ml @18:24:56.38 Intake.select :close @18:24:56.94 SSV.dial :exhaust @18:24:57.72 Exhaust.select :open @18:24:57.83 SS.seek 0ml @18:25:06.96 Exhaust.select :close @18:25:07.07 SSV.dial CBV @18:25:07.11 CBV.dial SSV @18:25:07.86 Allowing 130s for 25.0ml of remaining 925.0ml @18:25:07.88 Intake.select :open @18:25:10.05 Intake @16.1psia, vacuum from 10.0 to 13.0psi @18:25:10.07 SS.reconfigure :inhaling 0.47ml/s @18:25:10.11 SS.seek 25ml @18:25:11.61 PTV.dial :puck @18:25:12.36 PSV.dial PTV @18:25:13.25 PBV.dial :waste1 @18:25:13.68 PS.seek 1.5ml @18:25:19.70 delay 3 seconds @18:25:22.72 PBV.dial between PSV and waste1 @18:25:23.08 PS.seek 0ml @18:25:39.23 delay 2:00 @18:26:05.25 Stroke completed -- waiting for pressure to equalize ... @18:26:05.27 SS.reconfigure :exhale @18:26:05.31 SS.hold @18:26:28.21 Intake.select :close @18:26:28.37 SSV.dial :exhaust @18:26:29.13 Exhaust.select :open @18:26:29.25 SS.seek 0ml @18:26:38.50 Exhaust.select :close @18:26:38.62 SSV.dial CBV @18:26:38.66 CBV.dial SSV @18:26:39.39 Allowing 130s for 25.0ml of remaining 900.0ml @18:26:39.41 Intake.select :open @18:26:41.58 Intake @16.2psia, vacuum from 10.0 to 13.0psi @18:26:41.59 SS.reconfigure :inhaling 0.47ml/s @18:26:41.64 SS.seek 25ml @18:27:36.06 Stroke completed -- waiting for pressure to equalize ... @18:27:36.08 SS.reconfigure :exhale @18:27:36.12 SS.hold @18:27:39.25 recoverPSRreagent08 @18:27:39.26 PTV.dial :puck @18:27:39.34 PBV.dial :waste1 @18:27:40.07 delay 30 seconds @18:27:57.00 Intake.select :close @18:27:57.15 SSV.dial :exhaust @18:27:57.92 Exhaust.select :open @18:27:58.04 SS.seek 0ml @18:28:06.81 Exhaust.select :close @18:28:06.92 SSV.dial CBV @18:28:06.95 CBV.dial SSV @18:28:07.72 Allowing 130s for 25.0ml of remaining 875.0ml @18:28:07.74 Intake.select :open @18:28:09.90 Intake @16.1psia, vacuum from 10.0 to 13.0psi @18:28:09.92 SS.reconfigure :inhaling 0.47ml/s @18:28:09.96 SS.seek 25ml @18:28:10.10 PBV.dial between PSV and waste1 @18:28:10.46 PSR.select :air @18:28:10.48 PSV.dial PSR @18:28:11.24 PS.reconfigure :fast @18:28:11.28 PS.seek 2.5ml @18:28:35.16 PSV.dial PTV @18:28:35.93 delay 5 seconds @18:28:40.95 PSV.dial PSR @18:28:41.71 delay 5 seconds @18:28:46.73 PSV.dial PTV @18:28:47.53 delay 5 seconds @18:28:52.55 PSV.dial :air @18:28:53.31 PS.seek 0ml @18:29:05.50 Stroke completed -- waiting for pressure to equalize ... @18:29:05.51 SS.reconfigure :exhale @18:29:05.56 SS.hold @18:29:16.67 washCycleDA #2 of 2 @18:29:16.68 PSR.select :air @18:29:16.69 PSV.dial PSR @18:29:18.02 Pulling 0.5ml of PSR.wash @18:29:18.03 PSR.select :wash @18:29:18.21 PS.reconfigure :default @18:29:18.26 PS.seek 0.5ml @18:29:24.16 delay 5 seconds @18:29:25.93 Intake.select :close @18:29:26.09 SSV.dial :exhaust @18:29:27.31 Exhaust.select :open @18:29:27.43 SS.seek 0ml @18:29:29.18 PSR.select :air @18:29:29.29 PS.seek 2ml @18:29:36.23 Exhaust.select :close @18:29:36.34 SSV.dial CBV @18:29:36.39 CBV.dial SSV @18:29:37.12 Allowing 130s for 25.0ml of remaining 850.0ml @18:29:37.14 Intake.select :open @18:29:39.31 Intake @16.2psia, vacuum from 10.0 to 13.0psi @18:29:39.33 SS.reconfigure :inhaling 0.47ml/s @18:29:39.38 SS.seek 25ml @18:29:45.35 PTV.dial :puck @18:29:45.54 PSV.dial PTV @18:29:46.41 PBV.dial :waste1 @18:29:46.85 PS.seek 1.5ml @18:29:52.82 delay 3 seconds @18:29:55.84 PBV.dial between PSV and waste1 @18:29:56.22 PS.seek 0ml @18:30:12.35 delay 2:00 @18:30:35.07 Stroke completed -- waiting for pressure to equalize ... @18:30:35.09 SS.reconfigure :exhale @18:30:35.13 SS.hold @18:30:53.96 Intake.select :close @18:30:54.12 SSV.dial :exhaust @18:30:54.89 Exhaust.select :open @18:30:55.01 SS.seek 0ml @18:31:03.76 Exhaust.select :close @18:31:03.87 SSV.dial CBV @18:31:03.89 CBV.dial SSV @18:31:04.70 Allowing 130s for 25.0ml of remaining 825.0ml @18:31:04.72 Intake.select :open @18:31:06.88 Intake @16.2psia, vacuum from 10.0 to 13.0psi @18:31:06.90 SS.reconfigure :inhaling 0.47ml/s @18:31:06.94 SS.seek 25ml @18:32:02.57 Stroke completed -- waiting for pressure to equalize ... @18:32:02.59 SS.reconfigure :exhale @18:32:02.63 SS.hold @18:32:12.37 recoverPSRreagent08 @18:32:12.38 PTV.dial :puck @18:32:12.55 PBV.dial :waste1 @18:32:12.93 delay 30 seconds @18:32:22.93 Intake.select :close @18:32:23.09 SSV.dial :exhaust @18:32:23.88 Exhaust.select :open @18:32:24.00 SS.seek 0ml @18:32:32.84 Exhaust.select :close @18:32:32.95 SSV.dial CBV @18:32:32.97 CBV.dial SSV @18:32:33.78 Allowing 130s for 25.0ml of remaining 800.0ml @18:32:33.79 Intake.select :open @18:32:35.96 Intake @16.2psia, vacuum from 10.0 to 13.0psi @18:32:35.98 SS.reconfigure :inhaling 0.47ml/s @18:32:36.02 SS.seek 25ml @18:32:42.95 PBV.dial between PSV and waste1 @18:32:43.32 PSR.select :air @18:32:43.34 PSV.dial PSR @18:32:44.09 PS.reconfigure :fast @18:32:44.14 PS.seek 2.5ml @18:33:07.97 PSV.dial PTV @18:33:08.74 delay 5 seconds @18:33:13.76 PSV.dial PSR @18:33:14.52 delay 5 seconds @18:33:19.54 PSV.dial PTV @18:33:20.30 delay 5 seconds @18:33:25.32 PSV.dial :air @18:33:26.09 PS.seek 0ml @18:33:31.59 Stroke completed -- waiting for pressure to equalize ... @18:33:31.60 SS.reconfigure :exhale @18:33:31.65 SS.hold @18:33:49.47 getPMLReagent08 @18:33:49.48 PRV.dial :air @18:33:49.93 delay 5 seconds @18:33:52.50 Intake.select :close @18:33:52.66 SSV.dial :exhaust @18:33:53.42 Exhaust.select :open @18:33:53.54 SS.seek 0ml @18:33:54.94 PTV.dial :PRVmixing @18:33:56.27 delay 5 seconds @18:34:01.29 PRV.dial :ab2 @18:34:01.74 PTV.dial :PRVmixing @18:34:02.26 PSV.dial :mixloop @18:34:02.35 Exhaust.select :close @18:34:02.46 SSV.dial CBV @18:34:02.48 CBV.dial SSV @18:34:02.73 PS.reconfigure :default @18:34:02.77 PS.seek 0.3ml @18:34:03.28 Allowing 130s for 25.0ml of remaining 775.0ml @18:34:03.29 Intake.select :open @18:34:05.46 Intake @16.3psia, vacuum from 10.0 to 13.0psi @18:34:05.48 SS.reconfigure :inhaling 0.47ml/s @18:34:05.53 SS.seek 25ml @18:34:06.67 delay 2 seconds @18:34:08.68 PRV.dial :air @18:34:09.20 PS.seek 0.55ml @18:34:12.60 addPMLReagent08 @18:34:12.61 PSV.dial :air @18:34:13.09 PS.seek 2ml @18:34:28.75 PSV.dial :PML @18:34:29.22 PTV.dial :mixing @18:34:29.99 PBV.dial :waste1 @18:34:30.42 PS.seek 1.85ml @18:34:32.85 PBV.dial between PSV and waste1 @18:34:33.23 PS.seek 0ml @18:34:52.91 delay 2 seconds @18:34:54.93 delay 8:00 @18:35:01.12 Stroke completed -- waiting for pressure to equalize ... @18:35:01.13 SS.reconfigure :exhale @18:35:01.18 SS.hold @18:35:23.66 Intake.select :close @18:35:23.82 SSV.dial :exhaust @18:35:24.59 Exhaust.select :open @18:35:24.70 SS.seek 0ml @18:35:33.41 Exhaust.select :close @18:35:33.52 SSV.dial CBV @18:35:33.54 CBV.dial SSV @18:35:34.35 Allowing 130s for 25.0ml of remaining 750.0ml @18:35:34.37 Intake.select :open @18:35:36.53 Intake @16.2psia, vacuum from 10.0 to 13.0psi @18:35:36.55 SS.reconfigure :inhaling 0.47ml/s @18:35:36.59 SS.seek 25ml @18:36:32.10 Stroke completed -- waiting for pressure to equalize ... @18:36:32.11 SS.reconfigure :exhale @18:36:32.16 SS.hold @18:36:58.77 Intake.select :close @18:36:58.92 SSV.dial :exhaust @18:36:59.70 Exhaust.select :open @18:36:59.82 SS.seek 0ml @18:37:08.82 Exhaust.select :close @18:37:08.93 SSV.dial CBV @18:37:08.96 CBV.dial SSV @18:37:09.74 Allowing 130s for 25.0ml of remaining 725.0ml @18:37:09.75 Intake.select :open @18:37:11.92 Intake @16.1psia, vacuum from 10.0 to 13.0psi @18:37:11.94 SS.reconfigure :inhaling 0.47ml/s @18:37:11.99 SS.seek 25ml @18:38:07.13 Stroke completed -- waiting for pressure to equalize ... @18:38:07.14 SS.reconfigure :exhale @18:38:07.19 SS.hold @18:38:36.12 Intake.select :close @18:38:36.28 SSV.dial :exhaust @18:38:37.04 Exhaust.select :open @18:38:37.15 SS.seek 0ml @18:38:46.47 Exhaust.select :close @18:38:46.58 SSV.dial CBV @18:38:46.60 CBV.dial SSV @18:38:47.40 Allowing 130s for 25.0ml of remaining 700.0ml @18:38:47.41 Intake.select :open @18:38:49.58 Intake @16.3psia, vacuum from 10.0 to 13.0psi @18:38:49.60 SS.reconfigure :inhaling 0.47ml/s @18:38:49.65 SS.seek 25ml @18:39:41.26 Vacuum too high -- waiting up to 40 seconds @18:39:41.28 SS.reconfigure :exhale @18:39:41.33 SS.hold @18:39:55.89 Intake @18.0psia, vacuum from 10.0 to 13.0psi @18:39:55.90 SS.reconfigure :inhaling 0.38ml/s @18:39:55.95 SS.seek 25ml @18:40:01.01 Stroke completed -- waiting for pressure to equalize ... @18:40:01.09 SS.reconfigure :exhale @18:40:01.13 SS.hold @18:40:19.49 Intake.select :close @18:40:19.65 SSV.dial :exhaust @18:40:20.41 Exhaust.select :open @18:40:20.53 SS.seek 0ml @18:40:29.36 Exhaust.select :close @18:40:29.48 SSV.dial CBV @18:40:29.50 CBV.dial SSV @18:40:30.34 Allowing 130s for 25.0ml of remaining 675.0ml @18:40:30.35 Intake.select :open @18:40:32.52 Intake @16.2psia, vacuum from 10.0 to 13.0psi @18:40:32.54 SS.reconfigure :inhaling 0.38ml/s @18:40:32.59 SS.seek 25ml @18:41:40.73 Stroke completed -- waiting for pressure to equalize ... @18:41:40.75 SS.reconfigure :exhale @18:41:40.79 SS.hold @18:42:11.47 Intake.select :close @18:42:11.62 SSV.dial :exhaust @18:42:12.39 Exhaust.select :open @18:42:12.51 SS.seek 0ml @18:42:21.51 Exhaust.select :close @18:42:21.62 SSV.dial CBV @18:42:21.66 CBV.dial SSV @18:42:22.42 Allowing 130s for 25.0ml of remaining 650.0ml @18:42:22.44 Intake.select :open @18:42:24.61 Intake @16.2psia, vacuum from 10.0 to 13.0psi @18:42:24.63 SS.reconfigure :inhaling 0.38ml/s @18:42:24.67 SS.seek 25ml @18:42:54.95 recoverPRVreagent08 @18:42:54.96 PTV.dial :mixing @18:42:55.04 PBV.dial :waste1 @18:42:55.42 delay 30 seconds @18:43:25.44 PBV.dial between PSV and waste1 @18:43:25.80 PSV.dial :air @18:43:26.28 PS.reconfigure :fast @18:43:26.33 PS.seek 2.5ml @18:43:32.45 Stroke completed -- waiting for pressure to equalize ... @18:43:32.46 SS.reconfigure :exhale @18:43:32.51 SS.hold @18:43:50.24 PSV.dial :mixloop @18:43:50.71 delay 2 seconds @18:43:52.73 PSV.dial :air @18:43:53.19 delay 2 seconds @18:43:55.21 PSV.dial :mixloop @18:43:55.68 delay 2 seconds @18:43:57.69 PSV.dial :air @18:43:58.17 PS.seek 0ml @18:44:09.73 Intake.select :close @18:44:09.89 SSV.dial :exhaust @18:44:10.65 Exhaust.select :open @18:44:10.77 SS.seek 0ml @18:44:19.62 Exhaust.select :close @18:44:19.73 SSV.dial CBV @18:44:19.77 CBV.dial SSV @18:44:20.60 Allowing 130s for 25.0ml of remaining 625.0ml @18:44:20.61 Intake.select :open @18:44:21.71 washCycleDA #1 of 3 @18:44:21.72 PSR.select :air @18:44:21.74 PSV.dial PSR @18:44:22.78 Intake @16.3psia, vacuum from 10.0 to 13.0psi @18:44:22.80 SS.reconfigure :inhaling 0.38ml/s @18:44:22.85 SS.seek 25ml @18:44:23.09 Pulling 0.5ml of PSR.wash @18:44:23.10 PSR.select :wash @18:44:23.22 PS.reconfigure :default @18:44:23.26 PS.seek 0.5ml @18:44:29.21 delay 5 seconds @18:44:34.23 PSR.select :air @18:44:34.35 PS.seek 2ml @18:44:50.58 PTV.dial :puck @18:44:51.33 PSV.dial PTV @18:44:52.08 PBV.dial :waste1 @18:44:52.50 PS.seek 1.5ml @18:44:58.49 delay 3 seconds @18:45:01.51 PBV.dial between PSV and waste1 @18:45:01.87 PS.seek 0ml @18:45:16.86 Vacuum too high -- waiting up to 40 seconds @18:45:16.87 SS.reconfigure :exhale @18:45:16.93 SS.hold @18:45:18.05 delay 2:00 @18:45:35.90 Intake @17.4psia, vacuum from 10.0 to 13.0psi @18:45:35.91 SS.reconfigure :inhaling 0.31ml/s @18:45:35.96 SS.seek 25ml @18:45:54.77 Stroke completed -- waiting for pressure to equalize ... @18:45:54.79 SS.reconfigure :exhale @18:45:54.83 SS.hold @18:46:21.40 Intake.select :close @18:46:21.56 SSV.dial :exhaust @18:46:22.33 Exhaust.select :open @18:46:22.45 SS.seek 0ml @18:46:31.33 Exhaust.select :close @18:46:31.43 SSV.dial CBV @18:46:31.46 CBV.dial SSV @18:46:32.24 Allowing 130s for 25.0ml of remaining 600.0ml @18:46:32.26 Intake.select :open @18:46:34.43 Intake @16.2psia, vacuum from 10.0 to 13.0psi @18:46:34.44 SS.reconfigure :inhaling 0.31ml/s @18:46:34.49 SS.seek 25ml @18:47:18.07 recoverPSRreagent08 @18:47:18.08 PTV.dial :puck @18:47:18.10 PBV.dial :waste1 @18:47:18.47 delay 30 seconds @18:47:48.50 PBV.dial between PSV and waste1 @18:47:48.88 PSR.select :air @18:47:48.90 PSV.dial PSR @18:47:49.67 PS.reconfigure :fast @18:47:49.71 PS.seek 2.5ml @18:48:01.63 Stroke completed -- waiting for pressure to equalize ... @18:48:01.64 SS.reconfigure :exhale @18:48:01.69 SS.hold @18:48:13.76 PSV.dial PTV @18:48:14.53 delay 5 seconds @18:48:19.55 PSV.dial PSR @18:48:20.31 delay 5 seconds @18:48:25.33 PSV.dial PTV @18:48:26.10 delay 5 seconds @18:48:31.11 PSV.dial :air @18:48:32.01 PS.seek 0ml @18:48:41.87 SS.reconfigure :default @18:48:42.33 SS.hold @18:48:42.45 I2C::Bus::Error in core_reader -- Unexpected ACK byte: 0x43 @18:48:42.71 ShallowSampler::Clogged in MAIN -- Filtering too Slowly @18:48:42.72 Waiting to Resume from Checkpoint "Filtering too Slowly" @18:48:42.74 BadNews.email "Filtering too Slowly",Subject: "ShallowSampler::Clogged in MAIN" @18:48:42.98 Polling Can every 10:00 @18:48:47.59 upsync @18:48:55.53 washCycleDA #2 of 3 @18:48:55.54 PSR.select :air @18:48:55.56 PSV.dial PSR @18:48:56.91 Pulling 0.5ml of PSR.wash @18:48:56.92 PSR.select :wash @18:48:57.05 PS.reconfigure :default @18:48:57.10 PS.seek 0.5ml @18:49:03.02 delay 5 seconds @18:49:08.04 PSR.select :air @18:49:08.16 PS.seek 2ml @18:49:24.36 PTV.dial :puck @18:49:24.37 PSV.dial PTV @18:49:25.14 PBV.dial :waste1 @18:49:25.58 PS.seek 1.5ml @18:49:31.49 delay 3 seconds @18:49:34.51 PBV.dial between PSV and waste1 @18:49:34.88 PS.seek 0ml @18:49:51.05 delay 2:00 @18:51:51.08 recoverPSRreagent08 @18:51:51.09 PTV.dial :puck @18:51:51.10 PBV.dial :waste1 @18:51:51.47 delay 30 seconds @18:52:21.50 PBV.dial between PSV and waste1 @18:52:21.87 PSR.select :air @18:52:21.89 PSV.dial PSR @18:52:22.66 PS.reconfigure :fast @18:52:22.71 PS.seek 2.5ml @18:52:46.57 PSV.dial PTV @18:52:47.47 delay 5 seconds @18:52:52.49 PSV.dial PSR @18:52:53.23 delay 5 seconds @18:52:58.25 PSV.dial PTV @18:52:59.01 delay 5 seconds @18:53:04.04 PSV.dial :air @18:53:04.92 PS.seek 0ml @18:53:28.30 washCycleDA #3 of 3 @18:53:28.31 PSR.select :air @18:53:28.32 PSV.dial PSR @18:53:29.67 Pulling 0.5ml of PSR.wash @18:53:29.68 PSR.select :wash @18:53:29.79 PS.reconfigure :default @18:53:29.84 PS.seek 0.5ml @18:53:36.11 delay 5 seconds @18:53:41.13 PSR.select :air @18:53:41.25 PS.seek 2ml @18:53:57.45 PTV.dial :puck @18:53:57.46 PSV.dial PTV @18:53:58.23 PBV.dial :waste1 @18:53:58.67 PS.seek 1.5ml @18:54:04.69 delay 3 seconds @18:54:07.71 PBV.dial between PSV and waste1 @18:54:08.10 PS.seek 0ml @18:54:24.23 delay 2:00 @18:56:24.26 recoverPSRreagent08 @18:56:24.27 PTV.dial :puck @18:56:24.28 PBV.dial :waste1 @18:56:24.65 delay 30 seconds @18:56:54.68 PBV.dial between PSV and waste1 @18:56:55.05 PSR.select :air @18:56:55.07 PSV.dial PSR @18:56:55.83 PS.reconfigure :fast @18:56:55.88 PS.seek 2.5ml @18:57:19.79 PSV.dial PTV @18:57:20.56 delay 5 seconds @18:57:25.58 PSV.dial PSR @18:57:26.34 delay 5 seconds @18:57:31.36 PSV.dial PTV @18:57:32.12 delay 5 seconds @18:57:37.14 PSV.dial :air @18:57:37.90 PS.seek 0ml @18:58:01.22 Gate.power :camera,:ON @18:58:01.24 Mixing DA Substrates @18:58:01.25 PSR.select :air @18:58:01.27 PSV.dial PSR @18:58:02.69 Pulling 0.25ml of PSR.sub1 @18:58:02.71 PSR.select :sub1 @18:58:02.91 PS.reconfigure :default @18:58:03.01 PS.seek 0.25ml @18:58:06.65 delay 5 seconds @18:58:11.67 Pulling 0.25ml of PSR.sub2 @18:58:11.72 PSR.select :sub2 @18:58:11.83 PS.seek 0.5ml @18:58:15.29 delay 5 seconds @18:58:20.31 PSR.select :air @18:58:20.42 PS.seek 2ml @18:58:36.63 PTV.dial :puck @18:58:36.64 PSV.dial PTV @18:58:37.43 PBV.dial :waste1 @18:58:37.86 PS.seek 1.5ml @18:58:43.88 delay 3 seconds @18:58:46.90 PBV.dial between PSV and waste1 @18:58:47.29 PS.seek 0ml @18:59:03.38 delay 10 seconds @18:59:13.41 recoverPSRreagent08 @18:59:13.42 PTV.dial :puck @18:59:13.43 PBV.dial :waste1 @18:59:13.80 delay 30 seconds @18:59:43.83 PBV.dial between PSV and waste1 @18:59:44.21 PSR.select :air @18:59:44.23 PSV.dial PSR @18:59:45.01 PS.reconfigure :fast @18:59:45.06 PS.seek 2.5ml @19:00:09.25 PSV.dial PTV @19:00:10.04 delay 5 seconds @19:00:15.06 PSV.dial PSR @19:00:15.82 delay 5 seconds @19:00:20.84 PSV.dial PTV @19:00:21.74 delay 5 seconds @19:00:26.76 PSV.dial :air @19:00:27.68 PS.seek 0ml @19:00:51.63 Additional air clear @19:00:51.64 PSR.select :air @19:00:51.66 PSV.dial PSR @19:00:53.03 Pulling 2ml of PSR.air @19:00:53.04 PSR.select :air @19:00:53.07 PS.reconfigure :default @19:00:53.11 PS.seek 2ml @19:01:14.42 delay 5 seconds @19:01:19.44 PTV.dial :puck @19:01:19.46 PSV.dial PTV @19:01:20.83 PBV.dial :waste1 @19:01:21.28 PS.seek 1.5ml @19:01:27.22 delay 3 seconds @19:01:30.24 PBV.dial between PSV and waste1 @19:01:30.61 delay 1 second @19:01:31.63 PSV.dial :waste1 @19:01:32.70 PS.seek 0ml @19:01:48.87 Processing Puck Heater at 28C before imaging @19:01:48.88 PH.coast @19:01:48.98 Imaging da puck in Processing Clamp while storing it to tube 5 @19:01:49.16 SC.reconfigure :tight @19:01:49.20 SC.seek 5 @19:01:52.78 SC.configure :limp @19:01:52.99 Elbow.reconfigure :tight @19:01:53.04 Elbow.seek 5 @19:01:54.79 Forearm.reconfigure :tight @19:01:54.84 Forearm.seek 5 + 450 counts @19:01:55.86 SE.reconfigure :pick @19:01:55.92 SE.seek :beamBreak @19:01:57.31 SE.configure :default @19:01:57.47 Elbow.reconfigure :limp @19:01:57.52 Forearm.reconfigure :loose @19:01:57.57 Forearm.seek 5 @19:01:57.85 Elbow.hold @19:01:57.91 Elbow.reconfigure :tight @19:01:58.02 SE.seek 25.13 Pucks below Top Plate @19:01:58.48 Forearm.force -20 @19:01:58.53 Forearm.seek 5 + 299 counts @19:01:58.81 Forearm.reconfigure :tight @19:01:58.86 Forearm.seek 5 + 450 counts @19:01:59.09 SE.seek 26.13 Pucks below Top Plate @19:01:59.65 Forearm.reconfigure :loose @19:01:59.71 Forearm.seek :retract @19:02:00.65 Elbow.seek PC @19:02:03.61 Elbow.reconfigure :loose @19:02:03.72 Forearm.seek PC @19:02:04.71 Hand.close @19:02:05.37 PC.seek :open @19:02:10.17 Forearm.seek :retract @19:02:11.15 Elbow.reconfigure :tight @19:02:11.20 Elbow.seek 5 @19:02:13.81 Forearm.reconfigure :tight @19:02:13.86 Forearm.seek 5 + 450 counts @19:02:14.78 Hand.open @19:02:15.43 SE.seek 25.78 Pucks below Top Plate @19:02:15.71 SE.reconfigure :seal @19:02:15.76 SE.seek 24.82 Pucks below Top Plate @19:02:16.30 Lights, camera, ACTION! @19:02:16.33 PuckCamera: snap -bin=2,2 -auto /var/log/esp/da23dec1319h1000ml.tif @19:02:35.81 Waiting >20 seconds for da to relinquish FlushPuck @19:02:36.23 SX HX9/16 (USB:27): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 16.619 seconds /var/log/esp/da23dec1319h1000ml.tif: TIFF Upload Complete @19:02:36.26 PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da23dec1319h1000ml15s.tif @19:02:51.87 SX HX9/16 (USB:27): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15 seconds /var/log/esp/midres/da23dec1319h1000ml15s.tif: TIFF Upload Complete @19:02:51.89 PuckCamera: snap -bin=2,2 20.0 /var/log/esp/midres/da23dec1319h1000ml20s.tif @19:03:12.55 SX HX9/16 (USB:27): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 20 seconds /var/log/esp/midres/da23dec1319h1000ml20s.tif: TIFF Upload Complete @19:03:12.56 Gate.power :camera,:OFF @19:03:12.59 DAfans.email "16.619 sec auto-exposure",Subject: "da23dec1319h1000ml images!" @19:03:12.83 Elbow.seek 5 @19:03:13.25 Forearm.seek 5 + 450 counts @19:03:13.34 SE.reconfigure :gently @19:03:13.39 SE.seek 25.53 Pucks below Top Plate @19:03:14.10 Elbow.reconfigure :limp @19:03:14.17 Forearm.reconfigure :loose @19:03:14.22 Forearm.seek 5 @19:03:14.58 Elbow.hold @19:03:14.65 Elbow.reconfigure :tight @19:03:14.72 SE.reconfigure :default @19:03:14.77 SE.seek 25.72 Pucks below Top Plate @19:03:15.18 Forearm.force -20 @19:03:15.22 Forearm.seek 5 + 256 counts @19:03:16.45 Forearm.reconfigure :tight @19:03:16.57 Forearm.seek 5 + 450 counts @19:03:16.93 SE.seek :down @19:03:17.95 SE.stop @19:03:18.32 Forearm.reconfigure :loose @19:03:18.42 Forearm.seek :retract @19:03:19.50 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @19:03:19.88 Elbow.seek FlushPuck::Garage @19:03:21.85 Elbow.reconfigure :loose @19:03:22.11 Forearm.seek :garage @19:03:23.11 Hand.close @19:03:23.99 Forearm.seek :retract @19:03:25.04 Elbow.reconfigure :tight @19:03:25.13 Elbow.seek PC @19:03:26.50 Elbow.reconfigure :loose @19:03:26.70 Forearm.reconfigure :tight @19:03:26.81 Forearm.seek PC @19:03:28.36 PC.seek :closed @19:03:33.16 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.64 @19:03:33.30 Hand.open @19:03:34.13 Forearm.reconfigure :loose @19:03:34.22 Forearm.seek :retract @19:03:35.20 PSR.select :air @19:03:35.24 PSV.dial PSR @19:03:36.38 Pulling 0.5ml of PSR.flush @19:03:36.40 PSR.select :flush @19:03:36.58 PS.seek 0.5ml @19:03:42.68 delay 5 seconds @19:03:45.66 upsync @19:03:47.71 PSR.select :air @19:03:47.83 PS.seek 2.5ml @19:04:09.28 delay 1 second @19:04:10.30 PRV.dial :waste2,avoiding: :ab2 @19:04:12.17 PTV.dial :PRVmixing @19:04:13.50 PSV.dial :mixloop @19:04:14.54 PS.seek :empty @19:04:41.09 PSR.select :air @19:04:41.11 PSV.dial PSR @19:04:42.17 Pulling 0.5ml of PSR.flush @19:04:42.18 PSR.select :flush @19:04:42.30 PS.seek 0.5ml @19:04:48.25 delay 5 seconds @19:04:53.27 PSR.select :air @19:04:53.39 PS.seek 2.5ml @19:05:14.65 delay 1 second @19:05:15.67 PBV.dial :waste1 @19:05:16.04 PTV.dial :mixing @19:05:16.81 PSV.dial :mixloop @19:05:17.88 PS.seek :empty @19:05:44.28 PSV.dial PSR @19:05:45.32 PSR.select :flush @19:05:45.45 PS.seek 1ml @19:05:56.46 PSR.select :air @19:05:56.58 PS.seek 2.5ml @19:06:12.68 PSV.dial PTV @19:06:13.45 PTV.dial :puck @19:06:14.18 PBV.dial :waste1 @19:06:14.36 PS.seek 0ml @19:06:40.68 PSV.dial PSR @19:06:41.45 PSR.select :flush @19:06:41.57 PS.seek 0.5ml @19:06:47.52 PSR.select :air @19:06:47.64 PS.seek 2.5ml @19:07:08.96 PSV.dial PTV @19:07:09.73 PBV.dial PSV @19:07:10.30 PS.seek 0ml @19:07:36.59 PSV.dial PBV @19:07:37.06 PS.seek 2.5ml @19:08:03.49 PSV.dial :waste1 @19:08:04.24 PS.seek 0ml @19:08:30.62 PSV.dial PSR @19:08:31.09 PSR.select :air @19:08:31.11 PS.seek 2.5ml @19:08:57.55 PSV.dial PTV @19:08:58.48 PBV.dial PSV @19:08:58.67 PS.seek 0ml @19:09:25.10 PSV.dial PBV @19:09:25.62 PS.seek 2.5ml @19:09:52.19 PSV.dial :waste1 @19:09:53.06 PS.seek 0ml @19:10:19.52 PSV.dial PSR @19:10:19.99 PSR.select :flush @19:10:20.11 PS.seek 1ml @19:10:31.21 PSR.select :air @19:10:31.32 PS.seek 2.5ml @19:10:47.52 PSV.dial PTV @19:10:48.27 PTV.dial :puck @19:10:48.29 PBV.dial :waste1 @19:10:48.84 PS.seek 0ml @19:11:15.21 PSV.dial PSR @19:11:15.97 PSR.select :flush @19:11:16.10 PS.seek 0.5ml @19:11:22.08 PSR.select :air @19:11:22.20 PS.seek 2.5ml @19:11:43.52 PSV.dial PTV @19:11:44.29 PBV.dial PSV @19:11:44.85 PS.seek 0ml @19:12:11.24 PSV.dial PBV @19:12:11.72 PS.seek 2.5ml @19:12:38.02 PSV.dial :waste1 @19:12:38.78 PS.seek 0ml @19:13:05.14 PSV.dial PSR @19:13:05.61 PSR.select :air @19:13:05.64 PS.seek 2.5ml @19:13:31.96 PSV.dial PTV @19:13:32.71 PBV.dial PSV @19:13:32.89 PS.seek 0ml @19:13:59.30 PSV.dial PBV @19:13:59.90 PS.seek 2.5ml @19:14:26.24 PSV.dial :waste1 @19:14:27.01 PS.seek 0ml @19:14:53.39 Garaging FlushPuck in Processing Clamp @19:14:53.47 Elbow.reconfigure :tight @19:14:53.52 Elbow.seek PC @19:14:53.63 Elbow.reconfigure :loose @19:14:53.73 Forearm.seek PC @19:14:55.04 Hand.close @19:14:55.73 PC.seek :open @19:15:00.48 Forearm.seek :retract @19:15:01.48 Elbow.reconfigure :tight @19:15:01.53 Elbow.seek :garage @19:15:02.85 Elbow.reconfigure :loose @19:15:02.95 Forearm.reconfigure :tight @19:15:02.99 Forearm.seek :garage @19:15:04.04 Hand.open @19:15:04.66 Forearm.reconfigure :loose @19:15:04.71 Forearm.seek :retract @19:47:10.57 Session Begun @19:47:23.53 -> MainThread.resume @19:47:23.55
Resuming Thread after Filtering too Slowly at 18:48:42 # @19:47:23.60 SS.hold @19:47:23.71 Intake.select :close @19:47:23.89 ShallowSampler::Clogged in MAIN -- Intake @16psia & Outlet @14psia won't equalize after 425.0ml @19:47:23.90 SSV.dial :exhaust @19:47:24.70 Exhaust.select :open @19:47:24.84 SS.reconfigure :exhale @19:47:24.90 SS.seek 0ml @19:47:33.83 Exhaust.select :close @19:47:33.96 Sampled 425.0ml @19:47:33.97 Drying puck before fixing for 5:00 @19:47:33.99 CBV.dial :waste2b @19:47:34.80 delay 1:00 @19:48:34.82 CSV.dial :air @19:48:35.28 CS.reconfigure :fast @19:48:35.35 CS.seek 10ml @19:49:00.00 Pumping wcr puck from tube 6 up to 35psi... @19:49:00.01 CBV.dial SSV @19:49:01.35 CBV.dial between CSV and waste2b @19:49:02.34 CSV.dial between air and CTV @19:49:02.68 CS.seek 7.013ml @19:49:10.38 CTV.dial CSV,avoiding: :air @19:49:10.42
CSV.dial CTV @19:49:11.25 CS.reconfigure :pressurize @19:49:11.32 CS.seek 0ml @19:49:29.78 CSV.dial :air @19:49:30.24 CS.reconfigure :fast @19:49:30.31 CS.seek 10ml @19:49:55.15 CSV.dial between air and CTV @19:49:55.49 CS.seek 4.518ml @19:50:08.88 CTV.dial CSV,avoiding: :air @19:50:08.91
CSV.dial CTV @19:50:09.41 CS.reconfigure :pressurize @19:50:09.48 CS.seek 0ml @19:50:21.65 CSV.dial :air @19:50:22.11 CS.reconfigure :fast @19:50:22.18 CS.seek 10ml @19:50:46.65 CSV.dial between air and CTV @19:50:46.99 CS.seek 4.623ml @19:51:00.03 CTV.dial CSV,avoiding: :air @19:51:00.06
CSV.dial CTV @19:51:00.56 CS.reconfigure :pressurize @19:51:00.64 CS.seek 0ml @19:51:13.06 CSV.dial :air @19:51:13.52 CS.reconfigure :fast @19:51:13.59 CS.seek 10ml @19:51:38.08 CSV.dial between air and CTV @19:51:38.42 CS.seek 4.65ml @19:51:51.44 CTV.dial CSV,avoiding: :air @19:51:51.47
CSV.dial CTV @19:51:51.84 CS.reconfigure :pressurize @19:51:51.91 CS.seek 0ml @19:52:04.37 CSV.dial :air @19:52:04.84 CS.reconfigure :default @19:52:04.91 CS.hold @19:52:05.05 ShallowSampler::Error in MAIN -- Puck.from 6, :wcr reached only 18.5 of 35psi after pumping in 40ml of gas @19:52:05.07 Waiting to Resume from Checkpoint "Pumping wcr puck from tube 6 up to 35psi..." @19:52:05.10 BadNews.email "Puck.from 6, :wcr reached only 18.5 of 35psi after pumping in 40ml of gas",Subject: "ShallowSampler::Error in MAIN" @19:52:10.05 upsync @19:55:49.51 -> CC Collection Clamp contains wcr puck from tube 6 @19:55:51.84 -> PC Processing Clamp open @19:55:54.63 -> FLush @19:55:54.66 NameError in dave -- uninitialized constant FLush @19:55:57.34 -> Flush FlushPuck is in FlushPuck::Garage @19:56:50.21 -> CC Collection Clamp contains wcr puck from tube 6 @19:56:53.14 -> PC Processing Clamp open @19:57:46.53 -> Puck.count @19:57:46.62 SE.seek :down @19:57:46.71 SE.stop @19:57:46.83 SC.reconfigure :tight @19:57:46.90 SC.seek 1 @19:57:59.93 SC.configure :limp @19:58:00.17 Elbow.reconfigure :tight @19:58:00.24 Elbow.seek 1 @19:58:02.22 Forearm.reconfigure :tight @19:58:02.30 Forearm.seek 1 + 450 counts @19:58:03.33 SE.reconfigure :place @19:58:03.42 SE.seek :beamBreak @19:58:04.57 SE.configure :default @19:58:04.71 SE.seek :down @19:58:05.21 SE.stop @19:58:05.84 Forearm.reconfigure :loose @19:58:05.91 Forearm.seek :retract @19:58:06.92 SC.reconfigure :tight @19:58:06.99 SC.seek 2 @19:58:10.59 SC.configure :limp @19:58:10.77 Elbow.seek 2 @19:58:11.16 Forearm.reconfigure :tight @19:58:11.24 Forearm.seek 2 + 450 counts @19:58:12.33 SE.reconfigure :place @19:58:12.42 SE.seek :beamBreak @19:58:13.54 SE.configure :default @19:58:13.67 SE.seek :down @19:58:14.11 SE.stop @19:58:14.80 Forearm.reconfigure :loose @19:58:14.87 Forearm.seek :retract @19:58:16.32 SC.reconfigure :tight @19:58:16.40 SC.seek 3 @19:58:20.02 SC.configure :limp @19:58:20.20 Elbow.seek 3 @19:58:20.52 Forearm.reconfigure :tight @19:58:20.59 Forearm.seek 3 + 450 counts @19:58:21.69 SE.reconfigure :place @19:58:21.77 SE.seek :beamBreak @19:58:22.93 SE.configure :default @19:58:23.07 SE.seek :down @19:58:23.52 SE.stop @19:58:24.20 Forearm.reconfigure :loose @19:58:24.27 Forearm.seek :retract @19:58:25.26 SC.reconfigure :tight @19:58:25.34 SC.seek 4 @19:58:28.89 SC.configure :limp @19:58:29.08 Elbow.seek 4 @19:58:29.68 Forearm.reconfigure :tight @19:58:29.76 Forearm.seek 4 + 450 counts @19:58:30.79 SE.reconfigure :place @19:58:30.87 SE.seek :beamBreak @19:58:32.00 SE.configure :default @19:58:32.13 SE.seek :down @19:58:32.58 SE.stop @19:58:33.27 Forearm.reconfigure :loose @19:58:33.34 Forearm.seek :retract @19:58:34.33 SC.reconfigure :tight @19:58:34.41 SC.seek 5 @19:58:37.96 SC.configure :limp @19:58:38.14 Elbow.seek 5 @19:58:38.43 Forearm.reconfigure :tight @19:58:38.50 Forearm.seek 5 + 450 counts @19:58:39.68 SE.reconfigure :place @19:58:39.76 SE.seek :beamBreak @19:58:41.36 SE.configure :default @19:58:41.50 SE.seek :down @19:58:42.63 Forearm.reconfigure :loose @19:58:42.70 Forearm.seek :retract @19:58:42.83 SE.stop @19:58:43.67 SC.reconfigure :tight @19:58:43.74 SC.seek 6 @19:58:47.36 SC.configure :limp @19:58:47.55 Elbow.seek 6 @19:58:47.75 Forearm.reconfigure :tight @19:58:47.82 Forearm.seek 6 + 450 counts @19:58:48.91 SE.reconfigure :place @19:58:48.99 SE.seek :beamBreak @19:58:52.04 SE.configure :default @19:58:52.17 SE.seek :down @19:58:53.31 Forearm.reconfigure :loose @19:58:53.38 Forearm.seek :retract @19:58:54.53 SE.stop @19:58:54.59 SC.reconfigure :tight @19:58:54.66 SC.seek 7 @19:58:58.21 SC.configure :limp @19:58:58.39 Elbow.seek 7 @19:58:58.97 Forearm.reconfigure :tight @19:58:59.05 Forearm.seek 7 + 450 counts @19:59:00.16 SE.reconfigure :place @19:59:00.24 SE.seek :beamBreak @19:59:01.55 SE.configure :default @19:59:01.68 SE.seek :down @19:59:02.30 SE.stop @19:59:02.82 Forearm.reconfigure :loose @19:59:02.89 Forearm.seek :retract {1 => 33, 2 => 33, 3 => 33, 4 => 33, 5 => 27, 6 => 5, 7 => 31} @20:11:39.01 -> CC.unloadPuck 5, :WCR @20:11:39.09 Puck::Warning in dave -- Unloading Puck.from 6, :wcr to Storage Carousel @20:11:39.12 Storing WCR puck in Collection Clamp to tube 5 @20:11:39.32 SC.reconfigure :tight @20:11:39.39 SC.seek 5 @20:11:46.09 SC.configure :limp @20:11:46.26 Elbow.seek 5 @20:11:47.18 Forearm.reconfigure :tight @20:11:47.26 Forearm.seek 5 + 450 counts @20:11:48.31 SE.reconfigure :pick @20:11:48.39 SE.seek :beamBreak @20:11:49.79 SE.configure :default @20:11:49.98 Elbow.reconfigure :limp @20:11:50.05 Forearm.reconfigure :loose @20:11:50.11 Forearm.seek 5 @20:11:50.42 Elbow.hold @20:11:50.50 Elbow.reconfigure :tight @20:11:50.62 SE.seek 26.13 Pucks below Top Plate @20:11:51.13 Forearm.force -20 @20:11:51.18 Forearm.seek 5 + 280 counts @20:11:51.49 Forearm.reconfigure :tight @20:11:51.56 Forearm.seek 5 + 450 counts @20:11:51.91 SE.seek 27.13 Pucks below Top Plate @20:11:52.60 Forearm.reconfigure :loose @20:11:52.67 Forearm.seek :retract @20:11:53.65 Elbow.seek CC @20:11:55.35 Elbow.reconfigure :loose @20:11:55.46 Forearm.seek CC @20:11:56.41 Hand.close @20:11:57.06 CC.seek :open @20:12:01.83 Forearm.seek :retract @20:12:02.84 Elbow.reconfigure :tight @20:12:02.92 Elbow.seek 5 @20:12:04.69 Elbow.reconfigure :limp @20:12:04.76 Forearm.seek 5 @20:12:05.76 Elbow.hold @20:12:05.85 Elbow.reconfigure :tight @20:12:05.92 Hand.open @20:12:06.68 Forearm.force -20 @20:12:06.74 Forearm.seek 5 + 316 counts @20:12:07.04 Forearm.reconfigure :tight @20:12:07.11 Forearm.seek 5 + 450 counts @20:12:07.37 SE.seek :down @20:12:08.26 SE.stop @20:12:08.89 Forearm.reconfigure :loose @20:12:08.97 Forearm.seek :retract @20:12:28.37 -> Puck.count @20:12:28.45 SE.seek :down @20:12:28.54 SE.stop @20:12:28.66 SC.reconfigure :tight @20:12:28.73 SC.seek 1 @20:12:41.71 SC.configure :limp @20:12:41.89 Elbow.seek 1 @20:12:42.21 Forearm.reconfigure :tight @20:12:42.29 Forearm.seek 1 + 450 counts @20:12:43.34 SE.reconfigure :place @20:12:43.42 SE.seek :beamBreak @20:12:44.59 SE.configure :default @20:12:44.72 SE.seek :down @20:12:45.18 SE.stop @20:12:45.85 Forearm.reconfigure :loose @20:12:45.92 Forearm.seek :retract @20:12:46.89 SC.reconfigure :tight @20:12:46.96 SC.seek 2 @20:12:50.55 SC.configure :limp @20:12:50.73 Elbow.seek 2 @20:12:50.94 Forearm.reconfigure :tight @20:12:51.02 Forearm.seek 2 + 450 counts @20:12:52.06 SE.reconfigure :place @20:12:52.14 SE.seek :beamBreak @20:12:53.28 SE.configure :default @20:12:53.41 SE.seek :down @20:12:53.85 SE.stop @20:12:54.54 Forearm.reconfigure :loose @20:12:54.61 Forearm.seek :retract @20:12:55.58 SC.reconfigure :tight @20:12:55.65 SC.seek 3 @20:12:59.18 SC.configure :limp @20:12:59.36 Elbow.seek 3 @20:12:59.89 Forearm.reconfigure :tight @20:13:00.36 Forearm.seek 3 + 450 counts @20:13:01.54 SE.reconfigure :place @20:13:01.62 SE.seek :beamBreak @20:13:02.86 SE.configure :default @20:13:02.99 SE.seek :down @20:13:03.43 SE.stop @20:13:04.12 Forearm.reconfigure :loose @20:13:04.21 Forearm.seek :retract @20:13:05.23 SC.reconfigure :tight @20:13:05.31 SC.seek 4 @20:13:09.05 SC.configure :limp @20:13:09.24 Elbow.seek 4 @20:13:09.80 Forearm.reconfigure :tight @20:13:09.87 Forearm.seek 4 + 450 counts @20:13:10.94 SE.reconfigure :place @20:13:11.03 SE.seek :beamBreak @20:13:12.17 SE.configure :default @20:13:12.30 SE.seek :down @20:13:12.80 SE.stop @20:13:13.43 Forearm.reconfigure :loose @20:13:13.50 Forearm.seek :retract @20:13:14.48 SC.reconfigure :tight @20:13:14.55 SC.seek 5 @20:13:18.10 SC.configure :limp @20:13:18.27 Elbow.seek 5 @20:13:18.57 Forearm.reconfigure :tight @20:13:18.64 Forearm.seek 5 + 450 counts @20:13:19.70 SE.reconfigure :place @20:13:19.79 SE.seek :beamBreak @20:13:21.34 SE.configure :default @20:13:21.47 SE.seek :down @20:13:22.30 SE.stop @20:13:22.60 Forearm.reconfigure :loose @20:13:22.67 Forearm.seek :retract @20:13:23.69 SC.reconfigure :tight @20:13:23.76 SC.seek 6 @20:13:27.30 SC.configure :limp @20:13:27.94 Elbow.seek 6 @20:13:28.16 Forearm.reconfigure :tight @20:13:28.23 Forearm.seek 6 + 450 counts @20:13:29.26 SE.reconfigure :place @20:13:29.34 SE.seek :beamBreak @20:13:32.42 SE.configure :default @20:13:32.55 SE.seek :down @20:13:33.70 Forearm.reconfigure :loose @20:13:33.77 Forearm.seek :retract @20:13:34.93 SE.stop @20:13:34.99 SC.reconfigure :tight @20:13:35.06 SC.seek 7 @20:13:38.58 SC.configure :limp @20:13:38.76 Elbow.seek 7 @20:13:39.28 Forearm.reconfigure :tight @20:13:39.35 Forearm.seek 7 + 450 counts @20:13:40.44 SE.reconfigure :place @20:13:40.53 SE.seek :beamBreak @20:13:41.84 SE.configure :default @20:13:41.97 SE.seek :down @20:13:42.63 SE.stop @20:13:43.11 Forearm.reconfigure :loose @20:13:43.18 Forearm.seek :retract {1 => 33, 2 => 33, 3 => 33, 4 => 33, 5 => 28, 6 => 5, 7 => 31} @20:14:24.14 -> Flush FlushPuck is in FlushPuck::Garage @20:14:26.05 -> CC Collection Clamp open @20:14:27.99 -> PC Processing Clamp open @20:14:31.46 -> Flush.CC @20:14:31.48 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @20:14:31.69 Elbow.seek FlushPuck::Garage @20:14:33.54 Elbow.reconfigure :loose @20:14:33.65 Forearm.seek :garage @20:14:34.63 Hand.close @20:14:35.42 Forearm.seek :retract @20:14:36.45 Elbow.reconfigure :tight @20:14:36.52 Elbow.seek CC @20:14:39.43 Elbow.reconfigure :loose @20:14:39.54 Forearm.reconfigure :tight @20:14:39.61 Forearm.seek CC @20:14:41.29 CC.seek :closed @20:14:45.83 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.34 @20:14:45.91 Hand.open @20:14:46.62 Forearm.reconfigure :loose @20:14:46.69 Forearm.seek :retract FlushPuck::Garage @20:15:23.70 -> Flush.PC @20:15:23.72 Loading FlushPuck into Processing Clamp from Collection Clamp @20:15:23.87 Elbow.reconfigure :tight @20:15:23.94 Elbow.seek CC @20:15:24.22 Elbow.reconfigure :loose @20:15:24.34 Forearm.seek CC @20:15:25.30 Hand.close @20:15:25.93 CC.seek :open @20:15:30.58 Forearm.seek :retract @20:15:31.57 Elbow.reconfigure :tight @20:15:31.65 Elbow.seek PC @20:15:35.30 Elbow.reconfigure :loose @20:15:35.42 Forearm.reconfigure :tight @20:15:35.49 Forearm.seek PC @20:15:36.71 PC.seek :closed @20:15:41.39 Processing Clamp onSpring/offSpring for 16/26 samples, on/off torque 1.64 @20:15:41.47 Hand.open @20:15:42.12 Forearm.reconfigure :loose @20:15:42.18 Forearm.seek :retract Collection Clamp open @20:17:59.47 -> Flush.garage @20:17:59.49 Garaging FlushPuck in Processing Clamp @20:17:59.59 Elbow.reconfigure :tight @20:18:00.07 Elbow.seek PC @20:18:00.29 Elbow.reconfigure :loose @20:18:00.41 Forearm.seek PC @20:18:01.38 Hand.close @20:18:02.06 PC.seek :open @20:18:06.78 Forearm.seek :retract @20:18:07.79 Elbow.reconfigure :tight @20:18:07.86 Elbow.seek :garage @20:18:09.25 Elbow.reconfigure :loose @20:18:09.37 Forearm.reconfigure :tight @20:18:09.44 Forearm.seek :garage @20:18:10.49 Hand.open @20:18:11.24 Forearm.reconfigure :loose @20:18:11.31 Forearm.seek :retract FlushPuck::Garage @20:22:45.09 -> exit @20:22:45.10 Session Ended @01:05:54.07UTC14-Dec-23 Session Begun @01:06:05.74 -> abort! 'mission complete' @01:06:05.76 CmdServer::Client::Error in dave -- Aborting Mission: mission complete @01:06:05.80 BadNews.email "mission complete",Subject: "DA_WCR_121323 ABORTED!" # @01:06:10.14 upsync @01:06:10.23 Session Ended + ESPwhich DA_WCR_121423.rb + fn=DA_WCR_121423.rb + IFS=: + set /home/esp/esp2/mission . + fullPath=/home/esp/esp2/mission/DA_WCR_121423.rb + [ -f /home/esp/esp2/mission/DA_WCR_121423.rb ] + realpath /home/esp/esp2/mission/DA_WCR_121423.rb + return 0 + scriptPath=/home/esp/esp2local/mission/DA_WCR_121423.rb + exec ruby /home/esp/esp2/lib/esp.rb /home/esp/esp2local/mission/DA_WCR_121423.rb @13:20:55.34UTC14-Dec-23
ESP2 v3.42 I2C Gateway v3.20 for core bus @13:20:57.95 Configuration for short puck jake 1/11/21 by brent@mbari.org /home/esp/esp2/type/shallow/jake/configure.rb @13:21:01.58 Polling Can every 10:00 @13:21:01.78 Cleanly exited at 13:16:37UTC14-Dec-23 @13:21:01.83 Loading /home/esp/esp2local/mission/DA_WCR_121423.rb mission @13:21:01.89 Preparing to sleep until + 00:00:05 @13:21:01.90 GoodNews.email "Starting DA_WCR_121423 w/pucks in tube 6\n{1 => 33,\n 2 => 33,\n 3 => 33,\n 4 => 33,\n 5 => 28,\n 6 => 5,\n 7 => 31}\nCTD data unavailable\nCan@13:21:01, 31% flow, 16.2C, 34% humidity, 15.0psia, 13.720V, 0.327A, 220.789Ah, 4.48W",Subject: "DA_WCR_121423 begun" @13:21:01.94
Asleep til + 00:00:05 @13:21:01.98 Continuing at 13:21 @13:21:02.22 Gate.power :core,:OFF @13:21:02.24 Gate.power :raw,:OFF @13:21:02.25 Shutdown @13:21:05.52 GoodNews.email "Then, in tube 6: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_121423.rb:14\n{1 => 33,\n 2 => 33,\n 3 => 33,\n 4 => 33,\n 5 => 28,\n 6 => 5,\n 7 => 31}\nCTD data unavailable\nCan@13:21:02, 31% flow, 16.1C, 34% humidity, 15.1psia, 13.720V, 0.327A, 220.789Ah, 4.48W",Subject: "Asleep until 13:21" @13:21:06.89
Powering core bus @13:21:06.91 upsync @13:21:10.06 upsync @13:21:12.28
Gate.power :raw,:ON @13:21:13.30 CS.configure :default @13:21:13.35 PS.configure :default @13:21:13.39 SS.configure :default @13:21:13.44 CC.configure :default @13:21:13.49 PC.configure :default @13:21:13.55 Forearm.configure :loose @13:21:13.60 Elbow.configure :loose @13:21:13.67 SC.configure :loose @13:21:13.72 SE.configure :default @13:21:13.77 CH.configure :default @13:21:13.80 PH.configure :default @13:21:13.84 All dwarf controllers running firmware version 3.85 @13:21:13.95 Valve.reset @13:21:14.15 Intake.select :close @13:21:14.22 Exhaust.select :close @13:21:14.26 CTV.dial :air @13:21:14.35 PSV.dial :air @13:21:14.45
Forearm.home.seek :retract @13:21:15.22 Hand.close @13:21:15.25
Elbow.reconfigure :loose @13:21:15.35 Elbow.jog 1500 @13:21:15.46 PS.home.seek 0ml @13:21:15.59 CSV.dial :waste1 @13:21:15.86
Elbow.reconfigure :loose @13:21:15.91 Elbow.home.seek :clear @13:21:16.33 SSV.dial :spit,avoiding: :exhaust @13:21:17.19 CS.reconfigure :fast @13:21:17.22 SS.home.seek 0ml @13:21:17.31 CS.home.seek 0ml @13:21:19.22
SE.home.seek :down @13:21:19.84 SC.reconfigure :tight @13:21:19.89 SC.home.seek :initial @13:21:23.79 PS.stop @13:21:48.24
SC.configure :limp @13:21:48.30 Forearm.seek :retract @13:21:48.36 Elbow.seek :hammer @13:21:49.40 Forearm.reconfigure :tight @13:21:49.44 Forearm.seek retracted @13:21:50.12 PC.home.seek :open @13:21:50.14
CC.home.seek :open @13:21:53.55 Phase [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] @13:21:53.56 Notice.email "At 13:21:53 began in tube 6: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_121423.rb:14\n{1 => 33,\n 2 => 33,\n 3 => 33,\n 4 => 33,\n 5 => 28,\n 6 => 5,\n 7 => 31}\nCTD data unavailable\nCan@13:21:53, 31% flow, 16.2C, 34% humidity, 15.1psia, 13.739V, 0.290A, 220.795Ah, 3.99W",Subject: "Started DA_WCR_121423 phase initialPurge; da [1000, 1000] #da and wsr are run simultanelously" @13:21:53.59
Starting initialPurge @13:21:53.61 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @13:21:53.69 Hand.open @13:21:54.45 Elbow.reconfigure :tight @13:21:54.50 Elbow.seek FlushPuck::Garage @13:21:56.41 Elbow.reconfigure :loose @13:21:56.52 Forearm.reconfigure :loose @13:21:56.57 Forearm.seek :garage @13:21:57.66 Hand.close @13:21:58.03 upsync @13:21:58.46
Forearm.seek :retract @13:21:59.44 Elbow.reconfigure :tight @13:21:59.49 Elbow.seek CC @13:22:02.47 Elbow.reconfigure :loose @13:22:02.57 Forearm.reconfigure :tight @13:22:02.62 Forearm.seek CC @13:22:03.87 CC.seek :closed @13:22:08.44 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.43 @13:22:08.51 Hand.open @13:22:09.14 Forearm.reconfigure :loose @13:22:09.19 Forearm.seek :retract @13:22:10.14 Flushing the Collection Valve::Manifold @13:22:10.27 CSV.dial CSR @13:22:11.56 Pulling 5ml of CSR.flush @13:22:11.57 CSR.select :flush @13:22:11.69 CS.reconfigure :default @13:22:12.14 CS.seek 5ml @13:22:25.78 delay 5 seconds @13:22:30.80 CSR.select :air @13:22:30.96 CS.reconfigure :fast @13:22:31.01 CS.seek 7.501ml @13:22:37.78 CSV.dial :waste @13:22:39.08 CS.reconfigure :default @13:22:39.13 CS.seek 0ml @13:22:59.09 Purging external Intake @13:22:59.21 CSV.dial :flush @13:22:59.80 CS.reconfigure :fast @13:22:59.85 CS.seek 10ml @13:23:24.53 delay 5 seconds @13:23:29.55 CSV.dial CBV @13:23:29.60
CBV.dial CSV @13:23:30.34 CTV.dial :intake @13:23:31.10 Intake.select :open @13:23:31.22 CS.seek 0ml @13:23:54.96 Intake.select :close @13:23:55.07 CTV.dial :air @13:23:55.84 Flushing upper manifold @13:23:55.96 CSV.dial :flush @13:23:56.71 CS.seek 5ml @13:24:09.65 delay 5 seconds @13:24:14.67 CSV.dial :air @13:24:15.20 CS.seek 7ml @13:24:20.95 CSV.dial CBV @13:24:21.01
CBV.dial CSV @13:24:22.14 CTV.dial :air @13:24:22.15 CS.seek 0ml @13:24:38.84 delay 7 seconds @13:24:45.87 CS.seek 3ml @13:24:54.15 CSV.dial :waste @13:24:54.61 CS.seek 0ml @13:25:02.38 CSV.dial :flush @13:25:02.83 CS.seek 3ml @13:25:10.87 delay 5 seconds @13:25:15.89 CSV.dial :air @13:25:16.40 CS.seek 5.001ml @13:25:22.12 CSV.dial :air @13:25:22.30 CS.seek 0ml @13:25:34.47 delay 7 seconds @13:25:41.50 CS.seek 3ml @13:25:49.52 CSV.dial :waste @13:25:50.27 CS.seek 0ml @13:25:57.90 Garaging FlushPuck in Collection Clamp @13:25:57.97 Elbow.reconfigure :tight @13:25:58.02 Elbow.seek CC @13:25:58.33 Elbow.reconfigure :loose @13:25:58.43 Forearm.seek CC @13:25:59.43 Hand.close @13:26:00.08 CC.seek :open @13:26:05.11 Forearm.seek :retract @13:26:06.09 Elbow.reconfigure :tight @13:26:06.14 Elbow.seek :garage @13:26:09.23 Elbow.reconfigure :loose @13:26:09.32 Forearm.reconfigure :tight @13:26:09.37 Forearm.seek :garage @13:26:10.44 Hand.open @13:26:11.13 Forearm.reconfigure :loose @13:26:11.18 Forearm.seek :retract @13:26:12.20 da sampling at most 1000ml, wcr at most 1000ml @13:26:12.24 Loading sh1 puck from tube 6 into Collection Clamp @13:26:12.26 DAfans.email "Started da in tube 6 at 13:26:12UTC14-Dec-23\n{1 => 33,\n 2 => 33,\n 3 => 33,\n 4 => 33,\n 5 => 28,\n 6 => 5,\n 7 => 31}\nCTD data unavailable\nCan@13:21:53, 31% flow, 16.2C, 34% humidity, 15.1psia, 13.739V, 0.290A, 220.795Ah, 3.99W",Subject: "da sampling at most 1000ml, wcr at most 1000ml" @13:26:12.52
SC.reconfigure :tight @13:26:12.59 SC.seek 6 @13:26:16.60 upsync @13:26:19.42
SC.configure :limp @13:26:19.65 Elbow.reconfigure :tight @13:26:19.70 Elbow.seek 6 @13:26:21.60 Forearm.reconfigure :tight @13:26:21.66 Forearm.seek 6 + 450 counts @13:26:22.69 SE.reconfigure :pick @13:26:22.75 SE.seek :beamBreak @13:26:25.65 SE.configure :default @13:26:25.81 Elbow.seek 6 @13:26:26.01 Forearm.seek 6 + 300 counts @13:26:26.23 Forearm.reconfigure :loose @13:26:26.28 Hand.close @13:26:26.97 SE.seek :down @13:26:27.18
Forearm.seek :retract @13:26:28.69 Elbow.seek CC @13:26:29.49 SE.stop @13:26:30.30
Elbow.reconfigure :loose @13:26:30.40 Forearm.reconfigure :tight @13:26:30.45 Forearm.seek CC @13:26:31.72 CC.seek :closed @13:26:36.24 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.33 @13:26:36.25 Hand.open @13:26:36.89 Forearm.reconfigure :loose @13:26:36.94 Forearm.seek :retract @13:26:37.87 collectSample 1000ml w/bubble point of 24psi @13:26:37.89 Priming 7ml @13:26:37.90 Intake.select :close @13:26:37.91 Exhaust.select :close @13:26:37.92 SS.hold @13:26:38.12 Calibrating pressure and temperature sensors @13:26:38.15 CTV.dial :air @13:26:38.17 SSV.dial :spit,avoiding: :exhaust @13:26:38.25 SS.reconfigure :fast @13:26:38.30 SS.seek 5ml @13:26:41.16 SSV.dial CBV @13:26:41.18
CBV.dial SSV @13:26:42.54 delay 3.8 seconds @13:26:46.46 Sensor limits: Intake max 99.8psia; Outlet max 93.0psia @13:26:46.47 SSV.dial :spit @13:26:47.80 SS.seek 0ml @13:26:50.66 SSV.dial CBV @13:26:51.99 CTV.dial :intake @13:26:52.78 SSV.dial CBV @13:26:52.81
CBV.dial SSV @13:26:53.02 Allowing 40s for 7.0ml of remaining 7.0ml @13:26:53.03 Intake.select :open @13:26:55.20 Intake @14.8psia, vacuum from 9.3 to 12.3psi @13:26:55.21 SS.reconfigure :inhaling 2.20ml/s @13:26:55.26 SS.seek 7ml @13:27:02.02 Stroke completed -- waiting for pressure to equalize ... @13:27:02.03 SS.reconfigure :exhale @13:27:02.07 SS.hold @13:27:12.01 Intake.select :close @13:27:12.17 SSV.dial :exhaust @13:27:12.93 Exhaust.select :open @13:27:13.04 SS.seek 0ml @13:27:17.04 Exhaust.select :close @13:27:17.15 Sampling 1000.0ml @13:27:17.16 Intake.select :close @13:27:17.17 Exhaust.select :close @13:27:17.18 SS.reconfigure :default @13:27:17.23 SS.hold @13:27:17.44 CTV.dial :intake @13:27:17.51 SSV.dial CBV @13:27:17.53
CBV.dial SSV @13:27:18.33 Allowing 130s for 25.0ml of remaining 1000.0ml @13:27:18.34 Intake.select :open @13:27:20.51 Intake @15.5psia, vacuum from 10.0 to 13.0psi @13:27:20.52 SS.reconfigure :inhaling 2.20ml/s @13:27:20.57 SS.seek 25ml @13:27:28.62 Vacuum too high -- waiting up to 40 seconds @13:27:28.63 SS.reconfigure :exhale @13:27:28.68 SS.hold @13:27:34.33 Intake @16.9psia, vacuum from 10.0 to 13.0psi @13:27:34.34 SS.reconfigure :inhaling 1.76ml/s @13:27:34.39 SS.seek 25ml @13:27:38.03 Vacuum too high -- waiting up to 40 seconds @13:27:38.04 SS.reconfigure :exhale @13:27:38.09 SS.hold @13:27:40.83 Intake @17.2psia, vacuum from 10.0 to 13.0psi @13:27:40.85 SS.reconfigure :inhaling 1.41ml/s @13:27:40.89 SS.seek 25ml @13:27:45.03 Vacuum too high -- waiting up to 40 seconds @13:27:45.04 SS.reconfigure :exhale @13:27:45.09 SS.hold @13:27:47.24 Intake @16.7psia, vacuum from 10.0 to 13.0psi @13:27:47.26 SS.reconfigure :inhaling 1.13ml/s @13:27:47.30 SS.seek 25ml @13:27:51.31 Stroke completed -- waiting for pressure to equalize ... @13:27:51.32 SS.reconfigure :exhale @13:27:51.37 SS.hold @13:28:19.97 Intake.select :close @13:28:20.13 SSV.dial :exhaust @13:28:20.88 Exhaust.select :open @13:28:20.99 SS.seek 0ml @13:28:29.88 Exhaust.select :close @13:28:29.98 SSV.dial CBV @13:28:30.00
CBV.dial SSV @13:28:30.77 Allowing 130s for 25.0ml of remaining 975.0ml @13:28:30.78 Intake.select :open @13:28:32.95 Intake @16.1psia, vacuum from 10.0 to 13.0psi @13:28:32.96 SS.reconfigure :inhaling 1.13ml/s @13:28:33.01 SS.seek 25ml @13:28:54.12 Vacuum too high -- waiting up to 40 seconds @13:28:54.13 SS.reconfigure :exhale @13:28:54.18 SS.hold @13:28:59.55 Intake @17.6psia, vacuum from 10.0 to 13.0psi @13:28:59.57 SS.reconfigure :inhaling 0.91ml/s @13:28:59.62 SS.seek 25ml @13:29:04.85 Stroke completed -- waiting for pressure to equalize ... @13:29:04.86 SS.reconfigure :exhale @13:29:04.91 SS.hold @13:29:35.54 Intake.select :close @13:29:35.69 SSV.dial :exhaust @13:29:36.44 Exhaust.select :open @13:29:36.55 SS.seek 0ml @13:29:45.53 Exhaust.select :close @13:29:45.64 SSV.dial CBV @13:29:45.66
CBV.dial SSV @13:29:46.88 Allowing 130s for 25.0ml of remaining 950.0ml @13:29:46.89 Intake.select :open @13:29:49.05 Intake @16.0psia, vacuum from 10.0 to 13.0psi @13:29:49.06 SS.reconfigure :inhaling 0.91ml/s @13:29:49.11 SS.seek 25ml @13:30:18.92 Stroke completed -- waiting for pressure to equalize ... @13:30:18.93 SS.reconfigure :exhale @13:30:18.98 SS.hold @13:30:56.16 Intake.select :close @13:30:56.31 SSV.dial :exhaust @13:30:57.06 Exhaust.select :open @13:30:57.18 SS.seek 0ml @13:31:06.17 Exhaust.select :close @13:31:06.28 SSV.dial CBV @13:31:06.30
CBV.dial SSV @13:31:07.08 Allowing 130s for 25.0ml of remaining 925.0ml @13:31:07.09 Intake.select :open @13:31:09.26 Intake @16.0psia, vacuum from 10.0 to 13.0psi @13:31:09.27 SS.reconfigure :inhaling 0.91ml/s @13:31:09.32 SS.seek 25ml @13:31:38.79 Stroke completed -- waiting for pressure to equalize ... @13:31:38.80 SS.reconfigure :exhale @13:31:38.85 SS.hold @13:32:05.72 Intake.select :close @13:32:05.95 SSV.dial :exhaust @13:32:06.72 Exhaust.select :open @13:32:06.84 SS.seek 0ml @13:32:15.69 Exhaust.select :close @13:32:15.79 SSV.dial CBV @13:32:15.81
CBV.dial SSV @13:32:16.61 Allowing 130s for 25.0ml of remaining 900.0ml @13:32:16.62 Intake.select :open @13:32:18.79 Intake @16.3psia, vacuum from 10.0 to 13.0psi @13:32:18.80 SS.reconfigure :inhaling 0.91ml/s @13:32:18.85 SS.seek 25ml @13:32:48.65 Stroke completed -- waiting for pressure to equalize ... @13:32:48.66 SS.reconfigure :exhale @13:32:48.71 SS.hold @13:33:15.14 Intake.select :close @13:33:15.66 SSV.dial :exhaust @13:33:16.42 Exhaust.select :open @13:33:16.53 SS.seek 0ml @13:33:25.23 Exhaust.select :close @13:33:25.34 SSV.dial CBV @13:33:25.36
CBV.dial SSV @13:33:26.15 Allowing 130s for 25.0ml of remaining 875.0ml @13:33:26.16 Intake.select :open @13:33:28.33 Intake @16.2psia, vacuum from 10.0 to 13.0psi @13:33:28.34 SS.reconfigure :inhaling 0.91ml/s @13:33:28.39 SS.seek 25ml @13:33:58.35 Stroke completed -- waiting for pressure to equalize ... @13:33:58.36 SS.reconfigure :exhale @13:33:58.41 SS.hold @13:34:22.89 Intake.select :close @13:34:23.04 SSV.dial :exhaust @13:34:23.81 Exhaust.select :open @13:34:24.35 SS.seek 0ml @13:34:33.35 Exhaust.select :close @13:34:33.46 SSV.dial CBV @13:34:33.49
CBV.dial SSV @13:34:34.26 Allowing 130s for 25.0ml of remaining 850.0ml @13:34:34.27 Intake.select :open @13:34:36.44 Intake @16.2psia, vacuum from 10.0 to 13.0psi @13:34:36.46 SS.reconfigure :inhaling 0.91ml/s @13:34:36.51 SS.seek 25ml @13:35:05.76 Stroke completed -- waiting for pressure to equalize ... @13:35:05.77 SS.reconfigure :exhale @13:35:05.81 SS.hold @13:35:30.22 Intake.select :close @13:35:30.38 SSV.dial :exhaust @13:35:31.13 Exhaust.select :open @13:35:31.25 SS.seek 0ml @13:35:40.24 Exhaust.select :close @13:35:40.36 SSV.dial CBV @13:35:40.83
CBV.dial SSV @13:35:41.56 Allowing 130s for 25.0ml of remaining 825.0ml @13:35:41.57 Intake.select :open @13:35:43.73 Intake @16.1psia, vacuum from 10.0 to 13.0psi @13:35:43.74 SS.reconfigure :inhaling 0.91ml/s @13:35:43.79 SS.seek 25ml @13:36:13.02 Stroke completed -- waiting for pressure to equalize ... @13:36:13.03 SS.reconfigure :exhale @13:36:13.08 SS.hold @13:36:35.51 Intake.select :close @13:36:35.66 SSV.dial :exhaust @13:36:36.42 Exhaust.select :open @13:36:36.53 SS.seek 0ml @13:36:45.18 Exhaust.select :close @13:36:45.29 SSV.dial CBV @13:36:45.31
CBV.dial SSV @13:36:46.12 Allowing 130s for 25.0ml of remaining 800.0ml @13:36:46.13 Intake.select :open @13:36:48.30 Intake @16.3psia, vacuum from 10.0 to 13.0psi @13:36:48.31 SS.reconfigure :inhaling 0.91ml/s @13:36:48.36 SS.seek 25ml @13:37:16.64 Vacuum too high -- waiting up to 40 seconds @13:37:16.65 SS.reconfigure :exhale @13:37:16.70 SS.hold @13:37:24.30 Intake @17.4psia, vacuum from 10.0 to 13.0psi @13:37:24.32 SS.reconfigure :inhaling 0.73ml/s @13:37:24.36 SS.seek 25ml @13:37:26.87 Stroke completed -- waiting for pressure to equalize ... @13:37:26.88 SS.reconfigure :exhale @13:37:26.93 SS.hold @13:37:41.37 Intake.select :close @13:37:41.52 SSV.dial :exhaust @13:37:42.27 Exhaust.select :open @13:37:42.39 SS.seek 0ml @13:37:51.13 Exhaust.select :close @13:37:51.24 SSV.dial CBV @13:37:51.26
CBV.dial SSV @13:37:52.05 Allowing 130s for 25.0ml of remaining 775.0ml @13:37:52.06 Intake.select :open @13:37:54.23 Intake @16.2psia, vacuum from 10.0 to 13.0psi @13:37:54.25 SS.reconfigure :inhaling 0.73ml/s @13:37:54.29 SS.seek 25ml @13:38:30.54 Stroke completed -- waiting for pressure to equalize ... @13:38:30.55 SS.reconfigure :exhale @13:38:30.59 SS.hold @13:38:48.92 Intake.select :close @13:38:49.50 SSV.dial :exhaust @13:38:50.28 Exhaust.select :open @13:38:50.39 SS.seek 0ml @13:38:59.30 Exhaust.select :close @13:38:59.41 SSV.dial CBV @13:38:59.44
CBV.dial SSV @13:39:00.21 Allowing 130s for 25.0ml of remaining 750.0ml @13:39:00.22 Intake.select :open @13:39:02.39 Intake @16.3psia, vacuum from 10.0 to 13.0psi @13:39:02.41 SS.reconfigure :inhaling 0.73ml/s @13:39:02.46 SS.seek 25ml @13:39:38.47 Stroke completed -- waiting for pressure to equalize ... @13:39:38.48 SS.reconfigure :exhale @13:39:38.53 SS.hold @13:39:58.92 Intake.select :close @13:39:59.08 SSV.dial :exhaust @13:39:59.84 Exhaust.select :open @13:39:59.95 SS.seek 0ml @13:40:08.72 Exhaust.select :close @13:40:08.83 SSV.dial CBV @13:40:08.85
CBV.dial SSV @13:40:09.63 Allowing 130s for 25.0ml of remaining 725.0ml @13:40:09.64 Intake.select :open @13:40:11.81 Intake @16.3psia, vacuum from 10.0 to 13.0psi @13:40:11.82 SS.reconfigure :inhaling 0.73ml/s @13:40:11.87 SS.seek 25ml @13:40:47.01 Vacuum too high -- waiting up to 40 seconds @13:40:47.02 SS.reconfigure :exhale @13:40:47.08 SS.hold @13:40:54.64 Intake @16.3psia, vacuum from 10.0 to 13.0psi @13:40:54.65 SS.reconfigure :inhaling 0.59ml/s @13:40:54.70 SS.seek 25ml @13:40:56.06 Stroke completed -- waiting for pressure to equalize ... @13:40:56.07 SS.reconfigure :exhale @13:40:56.12 SS.hold @13:41:08.81 Intake.select :close @13:41:08.96 SSV.dial :exhaust @13:41:09.72 Exhaust.select :open @13:41:09.84 SS.seek 0ml @13:41:18.75 Exhaust.select :close @13:41:18.86 SSV.dial CBV @13:41:18.88
CBV.dial SSV @13:41:19.68 Allowing 130s for 25.0ml of remaining 700.0ml @13:41:19.69 Intake.select :open @13:41:21.85 Intake @16.3psia, vacuum from 10.0 to 13.0psi @13:41:21.86 SS.reconfigure :inhaling 0.59ml/s @13:41:21.91 SS.seek 25ml @13:42:06.53 Stroke completed -- waiting for pressure to equalize ... @13:42:06.54 SS.reconfigure :exhale @13:42:06.59 SS.hold @13:42:25.28 Intake.select :close @13:42:25.44 SSV.dial :exhaust @13:42:26.21 Exhaust.select :open @13:42:26.32 SS.seek 0ml @13:42:35.30 Exhaust.select :close @13:42:35.40 SSV.dial CBV @13:42:35.43
CBV.dial SSV @13:42:36.20 Allowing 130s for 25.0ml of remaining 675.0ml @13:42:36.22 Intake.select :open @13:42:38.38 Intake @16.2psia, vacuum from 10.0 to 13.0psi @13:42:38.40 SS.reconfigure :inhaling 0.59ml/s @13:42:38.45 SS.seek 25ml @13:43:22.67 Stroke completed -- waiting for pressure to equalize ... @13:43:22.68 SS.reconfigure :exhale @13:43:22.73 SS.hold @13:43:43.01 Intake.select :close @13:43:43.16 SSV.dial :exhaust @13:43:43.92 Exhaust.select :open @13:43:44.04 SS.seek 0ml @13:43:52.81 Exhaust.select :close @13:43:53.29 SSV.dial CBV @13:43:53.31
CBV.dial SSV @13:43:54.09 Allowing 130s for 25.0ml of remaining 650.0ml @13:43:54.11 Intake.select :open @13:43:56.28 Intake @16.3psia, vacuum from 10.0 to 13.0psi @13:43:56.29 SS.reconfigure :inhaling 0.59ml/s @13:43:56.34 SS.seek 25ml @13:44:41.15 Stroke completed -- waiting for pressure to equalize ... @13:44:41.16 SS.reconfigure :exhale @13:44:41.21 SS.hold @13:45:03.66 Intake.select :close @13:45:03.81 SSV.dial :exhaust @13:45:04.56 Exhaust.select :open @13:45:04.67 SS.seek 0ml @13:45:13.58 Exhaust.select :close @13:45:13.68 SSV.dial CBV @13:45:13.72
CBV.dial SSV @13:45:14.47 Allowing 130s for 25.0ml of remaining 625.0ml @13:45:14.48 Intake.select :open @13:45:16.65 Intake @16.3psia, vacuum from 10.0 to 13.0psi @13:45:16.66 SS.reconfigure :inhaling 0.59ml/s @13:45:17.14 SS.seek 25ml @13:45:59.41 Vacuum too high -- waiting up to 40 seconds @13:45:59.42 SS.reconfigure :exhale @13:45:59.48 SS.hold @13:46:10.62 Intake @17.4psia, vacuum from 10.0 to 13.0psi @13:46:10.64 SS.reconfigure :inhaling 0.47ml/s @13:46:10.69 SS.seek 25ml @13:46:13.34 Stroke completed -- waiting for pressure to equalize ... @13:46:13.35 SS.reconfigure :exhale @13:46:13.39 SS.hold @13:46:27.70 Intake.select :close @13:46:27.86 SSV.dial :exhaust @13:46:28.62 Exhaust.select :open @13:46:28.74 SS.seek 0ml @13:46:37.51 Exhaust.select :close @13:46:37.62 SSV.dial CBV @13:46:37.65
CBV.dial SSV @13:46:38.42 Allowing 130s for 25.0ml of remaining 600.0ml @13:46:38.43 Intake.select :open @13:46:40.60 Intake @16.3psia, vacuum from 10.0 to 13.0psi @13:46:40.61 SS.reconfigure :inhaling 0.47ml/s @13:46:40.66 SS.seek 25ml @13:47:36.20 Stroke completed -- waiting for pressure to equalize ... @13:47:36.21 SS.reconfigure :exhale @13:47:36.25 SS.hold @13:47:58.72 Intake.select :close @13:47:58.87 SSV.dial :exhaust @13:47:59.64 Exhaust.select :open @13:47:59.75 SS.seek 0ml @13:48:08.43 Exhaust.select :close @13:48:08.54 SSV.dial CBV @13:48:08.56
CBV.dial SSV @13:48:09.35 Allowing 130s for 25.0ml of remaining 575.0ml @13:48:09.37 Intake.select :open @13:48:11.53 Intake @16.3psia, vacuum from 10.0 to 13.0psi @13:48:11.54 SS.reconfigure :inhaling 0.47ml/s @13:48:11.64 SS.seek 25ml @13:49:07.21 Stroke completed -- waiting for pressure to equalize ... @13:49:07.22 SS.reconfigure :exhale @13:49:07.27 SS.hold @13:49:31.77 Intake.select :close @13:49:31.92 SSV.dial :exhaust @13:49:32.68 Exhaust.select :open @13:49:32.80 SS.seek 0ml @13:49:41.74 Exhaust.select :close @13:49:41.86 SSV.dial CBV @13:49:41.90
CBV.dial SSV @13:49:42.64 Allowing 130s for 25.0ml of remaining 550.0ml @13:49:42.65 Intake.select :open @13:49:44.81 Intake @16.4psia, vacuum from 10.0 to 13.0psi @13:49:44.83 SS.reconfigure :inhaling 0.47ml/s @13:49:44.88 SS.seek 25ml @13:50:39.93 Stroke completed -- waiting for pressure to equalize ... @13:50:39.94 SS.reconfigure :exhale @13:50:39.98 SS.hold @13:51:09.01 Intake.select :close @13:51:09.16 SSV.dial :exhaust @13:51:09.91 Exhaust.select :open @13:51:10.03 SS.seek 0ml @13:51:18.82 Exhaust.select :close @13:51:18.93 SSV.dial CBV @13:51:18.95
CBV.dial SSV @13:51:19.73 Allowing 130s for 25.0ml of remaining 525.0ml @13:51:19.74 Intake.select :open @13:51:21.90 Intake @16.4psia, vacuum from 10.0 to 13.0psi @13:51:21.91 SS.reconfigure :inhaling 0.47ml/s @13:51:21.96 SS.seek 25ml @13:52:14.12 Vacuum too high -- waiting up to 40 seconds @13:52:14.14 SS.reconfigure :exhale @13:52:14.19 SS.hold @13:52:28.74 Intake @17.4psia, vacuum from 10.0 to 13.0psi @13:52:28.76 SS.reconfigure :inhaling 0.38ml/s @13:52:28.81 SS.seek 25ml @13:52:32.88 Stroke completed -- waiting for pressure to equalize ... @13:52:32.89 SS.reconfigure :exhale @13:52:32.94 SS.hold @13:52:51.29 Intake.select :close @13:52:51.45 SSV.dial :exhaust @13:52:52.21 Exhaust.select :open @13:52:52.32 SS.seek 0ml @13:53:01.08 Exhaust.select :close @13:53:01.19 SSV.dial CBV @13:53:01.21
CBV.dial SSV @13:53:02.01 Allowing 130s for 25.0ml of remaining 500.0ml @13:53:02.02 Intake.select :open @13:53:04.18 Intake @16.4psia, vacuum from 10.0 to 13.0psi @13:53:04.19 SS.reconfigure :inhaling 0.38ml/s @13:53:04.24 SS.seek 25ml @13:54:12.44 Stroke completed -- waiting for pressure to equalize ... @13:54:12.87 SS.reconfigure :exhale @13:54:12.92 SS.hold @13:54:41.40 Intake.select :close @13:54:41.55 SSV.dial :exhaust @13:54:42.32 Exhaust.select :open @13:54:42.43 SS.seek 0ml @13:54:51.42 Exhaust.select :close @13:54:51.53 SSV.dial CBV @13:54:51.55
CBV.dial SSV @13:54:52.33 Allowing 130s for 25.0ml of remaining 475.0ml @13:54:52.34 Intake.select :open @13:54:54.94 Intake @16.4psia, vacuum from 10.0 to 13.0psi @13:54:54.95 SS.reconfigure :inhaling 0.38ml/s @13:54:55.00 SS.seek 25ml @13:56:03.55 Stroke completed -- waiting for pressure to equalize ... @13:56:03.56 SS.reconfigure :exhale @13:56:03.60 SS.hold @13:56:38.30 Intake.select :close @13:56:38.45 SSV.dial :exhaust @13:56:39.28 Exhaust.select :open @13:56:39.39 SS.seek 0ml @13:56:48.19 Exhaust.select :close @13:56:48.29 SSV.dial CBV @13:56:48.33
CBV.dial SSV @13:56:49.08 Allowing 130s for 25.0ml of remaining 450.0ml @13:56:49.09 Intake.select :open @13:56:51.26 Intake @16.4psia, vacuum from 10.0 to 13.0psi @13:56:51.27 SS.reconfigure :inhaling 0.38ml/s @13:56:51.32 SS.seek 25ml @13:57:43.87 Vacuum too high -- waiting up to 40 seconds @13:57:43.88 SS.reconfigure :exhale @13:57:43.93 SS.hold @13:58:00.35 Intake @17.4psia, vacuum from 10.0 to 13.0psi @13:58:00.37 SS.reconfigure :inhaling 0.31ml/s @13:58:00.41 SS.seek 25ml @13:58:20.76 Stroke completed -- waiting for pressure to equalize ... @13:58:20.77 SS.reconfigure :exhale @13:58:20.82 SS.hold @13:58:47.57 Intake.select :close @13:58:47.73 SSV.dial :exhaust @13:58:48.49 Exhaust.select :open @13:58:48.60 SS.seek 0ml @13:58:57.42 Exhaust.select :close @13:58:57.53 SSV.dial CBV @13:58:57.55
CBV.dial SSV @13:58:58.33 Allowing 130s for 25.0ml of remaining 425.0ml @13:58:58.34 Intake.select :open @13:59:00.50 Intake @16.3psia, vacuum from 10.0 to 13.0psi @13:59:00.51 SS.reconfigure :inhaling 0.31ml/s @13:59:00.56 SS.seek 25ml @14:00:27.01 Stroke completed -- waiting for pressure to equalize ... @14:00:27.02 SS.reconfigure :exhale @14:00:27.07 SS.hold @14:01:06.02 Intake.select :close @14:01:06.17 SSV.dial :exhaust @14:01:06.93 Exhaust.select :open @14:01:07.10 SS.seek 0ml @14:01:16.23 Exhaust.select :close @14:01:16.34 SSV.dial CBV @14:01:16.36
CBV.dial SSV @14:01:17.56 Allowing 130s for 25.0ml of remaining 400.0ml @14:01:17.57 Intake.select :open @14:01:19.74 Intake @16.4psia, vacuum from 10.0 to 13.0psi @14:01:19.76 SS.reconfigure :inhaling 0.31ml/s @14:01:19.80 SS.seek 25ml @14:02:46.65 Stroke completed -- waiting for pressure to equalize ... @14:02:46.66 SS.reconfigure :exhale @14:02:46.71 SS.hold @14:03:26.89 SS.reconfigure :default @14:03:26.94 SS.hold @14:03:27.00 Intake.select :close @14:03:27.16 ShallowSampler::Clogged in MAIN -- Intake @16psia & Outlet @15psia won't equalize after 625.0ml @14:03:27.17 SSV.dial :exhaust @14:03:27.92 Exhaust.select :open @14:03:28.02 SS.reconfigure :exhale @14:03:28.07 SS.seek 0ml @14:03:36.88 Exhaust.select :close @14:03:36.99 Sampled 625.0ml @14:03:37.01 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) Intake.select :close @14:03:37.08 CSV.dial :waste @14:03:37.22 SSV.dial CBV,avoiding: :exhaust @14:03:37.24
CBV.dial SSV @14:03:37.38 CS.seek 0ml @14:03:37.46 CSV.dial :air @14:03:38.22 CS.seek 10ml @14:03:39.15
CTV.dial :air @14:03:39.93 delay 15 seconds @14:03:54.95 SSV.dial :spit,avoiding: :exhaust @14:03:56.28 CTV.dial CSV,avoiding: :intake @14:03:56.31
SS.seek 0ml @14:03:56.43 SSV.dial CBV,avoiding: :exhaust @14:04:02.84 SSV.dial between CBV and plug @14:04:03.33 CBV.dial between SSV and waste2 @14:04:03.70 CSV.dial between air and CTV @14:04:04.01 CS.seek 8.49ml @14:04:08.40 CTV.dial CSV,avoiding: :air @14:04:08.43
CSV.dial CTV @14:04:08.78 CS.reconfigure :pressurize @14:04:08.82 CS.seek 0ml @14:04:23.72 BubblePoint: Intake @24.3psia, Outlet @0.7psia @14:04:23.73 CBV.dial SSV @14:04:24.07 SS.reconfigure :default @14:04:24.12 SS.seek 10ml @14:04:29.84 delay 15 seconds @14:04:44.97 Evacuated: Intake @23.7psia, Outlet @23.3psia @14:04:44.98 SSV.dial :spit,avoiding: :exhaust @14:04:46.01 SS.reconfigure :exhale @14:04:46.06 SS.seek 0ml @14:04:51.14 SS.reconfigure :fast @14:04:51.18 SS.seek 5ml @14:04:53.98 SSV.dial CBV,avoiding: :exhaust @14:04:54.01
CBV.dial SSV @14:04:55.34 CTV.dial :air,avoiding: :intake @14:04:56.68 CSV.dial CTV @14:04:56.71
SS.seek 10ml @14:04:57.92 CSV.dial :air @14:04:58.48 CS.reconfigure :fast @14:04:58.53 CS.seek 0ml @14:04:59.24
SSV.dial :spit,avoiding: :exhaust @14:05:00.56 SS.reconfigure :exhale @14:05:00.61 SS.seek 0ml @14:05:05.72 SS.reconfigure :fast @14:05:05.77 SS.seek 5ml @14:05:05.89 CS.stop @14:05:08.47 SSV.dial CBV,avoiding: :exhaust @14:05:08.49
CBV.dial SSV @14:05:10.83 SSV.dial :spit,avoiding: :exhaust @14:05:12.17 SS.reconfigure :default @14:05:12.22 SS.seek 0ml @14:05:15.41 SS.stop @14:05:15.45 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @14:05:15.46 Intake.select :close @14:05:15.53 CSV.dial :waste @14:05:15.66 SSV.dial CBV,avoiding: :exhaust @14:05:15.68
CBV.dial SSV @14:05:16.32 CS.seek 0ml @14:05:16.39 CSV.dial :air @14:05:17.06
CTV.dial :air @14:05:17.14 CS.seek 10ml @14:05:17.22
delay 15 seconds @14:05:32.24 SSV.dial :spit,avoiding: :exhaust @14:05:33.57 CTV.dial CSV,avoiding: :intake @14:05:33.61
SS.reconfigure :exhale @14:05:33.70 SS.seek 0ml @14:05:34.09 SSV.dial CBV,avoiding: :exhaust @14:05:41.62 SSV.dial between CBV and plug @14:05:42.12 CBV.dial between SSV and waste2 @14:05:42.53 CSV.dial between air and CTV @14:05:42.84 CS.seek 7.262ml @14:05:49.84 CTV.dial CSV,avoiding: :air @14:05:49.88
CSV.dial CTV @14:05:50.25 CS.reconfigure :pressurize @14:05:50.29 CS.seek 0ml @14:06:04.71 BubblePoint: Intake @24.2psia, Outlet @4.1psia @14:06:04.72 CBV.dial SSV @14:06:05.06 SS.reconfigure :default @14:06:05.10 SS.seek 10ml @14:06:10.68 delay 15 seconds @14:06:25.81 Evacuated: Intake @23.9psia, Outlet @23.6psia @14:06:25.82 SSV.dial :spit,avoiding: :exhaust @14:06:26.87 SS.reconfigure :exhale @14:06:26.92 SS.seek 0ml @14:06:31.97 SS.reconfigure :fast @14:06:32.02 SS.seek 5ml @14:06:35.15 SSV.dial CBV,avoiding: :exhaust @14:06:35.17
CBV.dial SSV @14:06:36.53 CTV.dial :air,avoiding: :intake @14:06:37.90 CSV.dial CTV @14:06:37.94
SS.seek 10ml @14:06:39.15 CSV.dial :air @14:06:39.59 CS.reconfigure :fast @14:06:39.63 CS.seek 0ml @14:06:40.65
SSV.dial :spit,avoiding: :exhaust @14:06:41.96 SS.reconfigure :exhale @14:06:42.01 SS.seek 0ml @14:06:44.74 CS.stop @14:06:47.09
SS.reconfigure :fast @14:06:47.15 SS.seek 5ml @14:06:49.97 SSV.dial CBV,avoiding: :exhaust @14:06:49.99
CBV.dial SSV @14:06:52.31 SSV.dial :spit,avoiding: :exhaust @14:06:53.64 SS.reconfigure :default @14:06:53.68 SS.seek 0ml @14:06:56.91 SS.stop @14:06:56.95 Lysing: Adding 2.0ml AqMeOH @14:06:56.96 CBV.dial between CSV and waste1,avoiding: :DBV @14:06:58.47 CTV.dial CSV @14:06:59.78 CSV.dial CSR @14:07:00.57 Pulling 0.999ml of CSR.AqMeOH @14:07:00.58 CSR.select :AqMeOH @14:07:00.69 CS.reconfigure :default @14:07:00.74 CS.seek 1ml @14:07:04.14 delay 5 seconds @14:07:09.16 CSR.select :air @14:07:09.45 CS.seek 6.001ml @14:07:23.11 delay 2 seconds @14:07:25.13 CSV.dial :waste2 @14:07:25.70 CS.reconfigure :fast @14:07:25.74 CS.seek 0ml @14:07:40.27 CSV.dial CSR @14:07:40.71 Pulling 2ml of CSR.AqMeOH CSR.select :AqMeOH @14:07:41.19 CS.reconfigure :default @14:07:41.30 CS.seek 2ml @14:07:47.21 delay 5 seconds @14:07:52.23 CSR.select :air @14:07:52.40 CS.seek 5ml @14:08:01.08 delay 2 seconds @14:08:03.10 CSV.dial CTV @14:08:03.66 CS.seek 1ml @14:08:15.00 Before lysing Collection Puck Heater at 12.9C/31.5psia @14:08:15.02 Heating Puck.from 6, :sh1 in Collection Clamp to 70C for 10:00 @14:08:15.04 CH.seek 70C,8:00 @14:10:31.83 CH.hold 10:00,70C @14:10:32.03 Begun lysing Collection Puck Heater at 68C/38.6psia @14:20:32.04 Ended lysing Collection Puck Heater at 70C/38.0psia @14:20:32.06 CS.seek 0ml @14:20:35.45 CTV.dial between spare and air,avoiding: :intake @14:20:36.49 CSV.dial :air @14:20:36.97 CS.seek 5ml @14:20:50.57 CSV.dial CTV @14:20:51.02 delay 2 seconds @14:20:53.04 CSV.dial :air @14:20:53.49 delay 2 seconds @14:20:55.51 CSV.dial CTV @14:20:55.96 delay 2 seconds @14:20:57.98 CSV.dial :air @14:20:58.43 delay 2 seconds @14:21:00.45 CS.seek 0ml @14:21:14.18 Puck @83.8psia after final lysis pressurization @14:21:14.20 Recovering Lysate into Collection syringe @14:21:14.21 CSV.dial CBV @14:21:15.24 CBV.dial CSV @14:21:15.61 CS.seek 5ml @14:21:29.44 delay 5 seconds @14:21:34.46 CTV.dial :air @14:21:34.99 CS.seek 6.999ml @14:21:40.88 delay 5 seconds @14:21:45.90 CSV.dial :air2 @14:21:46.34 CS.seek 10ml @14:21:55.29 CSV.dial between air and CTV,avoiding: CBV @14:21:56.51 Lysing Complete @14:21:56.58 Storing sh1 puck in Collection Clamp to tube 5 @14:21:56.76 SC.reconfigure :tight @14:21:56.81 SC.seek 5 @14:22:00.34 SC.configure :limp @14:22:00.55 Elbow.reconfigure :tight @14:22:00.59 Elbow.seek 5 @14:22:02.35 Forearm.reconfigure :tight @14:22:02.40 Forearm.seek 5 + 450 counts @14:22:03.44 SE.reconfigure :pick @14:22:03.49 SE.seek :beamBreak @14:22:04.76 SE.configure :default @14:22:04.92 Elbow.reconfigure :limp @14:22:04.97 Forearm.reconfigure :loose @14:22:05.02 Forearm.seek 5 @14:22:05.30 Elbow.hold @14:22:05.37 Elbow.reconfigure :tight @14:22:05.47 SE.seek 27.13 Pucks below Top Plate @14:22:05.94 Forearm.force -20 @14:22:05.98 Forearm.seek 5 + 286 counts @14:22:06.26 Forearm.reconfigure :tight @14:22:06.30 Forearm.seek 5 + 450 counts @14:22:06.58 SE.seek 28.13 Pucks below Top Plate @14:22:07.10 Forearm.reconfigure :loose @14:22:07.14 Forearm.seek :retract @14:22:08.08 Elbow.seek CC @14:22:09.75 Elbow.reconfigure :loose @14:22:09.85 Forearm.seek CC @14:22:10.80 Hand.close @14:22:11.41 CC.seek :open @14:22:16.61 Forearm.seek :retract @14:22:17.57 Elbow.reconfigure :tight @14:22:17.61 Elbow.seek 5 @14:22:19.37 Elbow.reconfigure :limp @14:22:19.42 Forearm.seek 5 @14:22:20.42 Elbow.hold @14:22:20.49 Elbow.reconfigure :tight @14:22:20.53 Hand.open @14:22:21.28 Forearm.force -20 @14:22:21.32 Forearm.seek 5 + 320 counts @14:22:21.65 Forearm.reconfigure :tight @14:22:21.70 Forearm.seek 5 + 450 counts @14:22:21.96 SE.seek :down @14:22:22.76 SE.stop @14:22:23.08
Forearm.reconfigure :loose @14:22:23.13 Forearm.seek :retract @14:22:24.05 Tranferring DA @14:22:24.06 Beginning samplePassC @14:22:24.08 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:22:24.26 Elbow.seek FlushPuck::Garage @14:22:26.15 Elbow.reconfigure :loose @14:22:26.26 Forearm.seek :garage @14:22:27.28 Hand.close @14:22:28.02 Forearm.seek :retract @14:22:29.05 Elbow.reconfigure :tight @14:22:29.10 Elbow.seek CC @14:22:32.01 Elbow.reconfigure :loose @14:22:32.11 Forearm.reconfigure :tight @14:22:32.58 Forearm.seek CC @14:22:33.94 CC.seek :closed @14:22:38.45 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.46 @14:22:38.52 Hand.open @14:22:39.22 Forearm.reconfigure :loose @14:22:39.27 Forearm.seek :retract @14:22:40.23 PSV.dial PSR @14:22:41.74 Pulling 1.5ml of PSR.flush PSR.select :flush @14:22:41.86 PS.seek 1.5ml @14:22:57.95 delay 5 seconds @14:23:02.97 PSV.dial :waste1 @14:23:03.45 PS.seek 0ml @14:23:19.57 PSV.dial PSR @14:23:20.04 PSR.select :air @14:23:20.16 PS.seek 2.5ml @14:23:46.45 PRV.dial CBV,avoiding: :ab2 @14:23:46.47
CBV.dial PRV @14:23:48.23 PSV.dial :mixloop @14:23:49.28 CTV.dial :air,avoiding: :intake @14:23:49.35 PTV.dial :PRVmixing @14:23:50.67 PS.seek 0ml @14:24:16.97 PSV.dial :air @14:24:17.56 PS.seek 2.5ml @14:24:43.91 PSV.dial :mixloop @14:24:44.37 PS.seek 0ml @14:25:10.75 PSV.dial :air @14:25:11.23 PS.seek 2.5ml @14:25:37.51 PSV.dial :mixloop @14:25:37.98 PS.seek 0ml @14:26:04.82 Garaging FlushPuck in Collection Clamp @14:26:04.89 Elbow.reconfigure :tight @14:26:04.94 Elbow.seek CC @14:26:05.28 Elbow.reconfigure :loose @14:26:05.38 Forearm.seek CC @14:26:06.42 Hand.close @14:26:07.03 CC.seek :open @14:26:11.65 Forearm.seek :retract @14:26:12.69 Elbow.reconfigure :tight @14:26:12.74 Elbow.seek :garage @14:26:15.57 Elbow.reconfigure :loose @14:26:15.67 Forearm.reconfigure :tight @14:26:15.72 Forearm.seek :garage @14:26:16.80 Hand.open @14:26:17.46 Forearm.reconfigure :loose @14:26:17.51 Forearm.seek :retract @14:26:18.51 PSV.dial :mixloop @14:26:18.58 PTV.dial :PRVmixing @14:26:18.66 PRV.dial CBV @14:26:18.69
CBV.dial PRV @14:26:18.88 CTV.dial :air,avoiding: :intake @14:26:18.96 Loading lyfil puck from tube 6 into Collection Clamp @14:26:19.19 SC.reconfigure :tight @14:26:19.23 SC.seek 6 @14:26:22.84 SC.configure :limp @14:26:23.05 Elbow.reconfigure :tight @14:26:23.10 Elbow.seek 6 @14:26:25.02 Forearm.reconfigure :tight @14:26:25.07 Forearm.seek 6 + 450 counts @14:26:26.10 SE.reconfigure :pick @14:26:26.16 SE.seek :beamBreak @14:26:29.50 SE.configure :default @14:26:29.66 Elbow.seek 6 @14:26:29.84 Forearm.seek 6 + 300 counts @14:26:30.06 Forearm.reconfigure :loose @14:26:30.11 Hand.close @14:26:30.67 SE.seek :down @14:26:30.86
Forearm.seek :retract @14:26:31.81 Elbow.seek CC @14:26:33.24 SE.stop @14:26:33.38
Elbow.reconfigure :loose @14:26:33.48 Forearm.reconfigure :tight @14:26:33.53 Forearm.seek CC @14:26:34.81 CC.seek :closed @14:26:39.32 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.24 @14:26:39.33 Hand.open @14:26:39.96 Forearm.reconfigure :loose @14:26:40.01 Forearm.seek :retract @14:26:40.98 CTV.dial CSV,avoiding: :intake @14:26:42.34 CSV.dial CTV @14:26:42.79 PS.seek 2.5ml @14:27:09.22 CS.seek 4.5ml @14:27:24.12 delay 8 seconds @14:27:32.15 PSV.dial :air @14:27:32.61 delay 2 seconds @14:27:35.01 PSV.dial :waste2 @14:27:35.81 PS.seek 0ml @14:28:02.19 CBV.dial between PRV and waste1 @14:28:02.52 CSV.dial :air @14:28:02.98 CS.seek 10ml @14:28:17.93 CSV.dial CTV @14:28:18.40 CS.seek 0ml @14:28:44.87 CBV.dial :waste1 @14:28:45.19 delay 5 seconds @14:28:50.21 CTV.dial :air,avoiding: :intake @14:28:51.62 Storing lyfil puck in Collection Clamp to tube 5 @14:28:51.80 SC.reconfigure :tight @14:28:51.84 SC.seek 5 @14:28:55.39 SC.configure :limp @14:28:55.60 Elbow.reconfigure :tight @14:28:55.65 Elbow.seek 5 @14:28:57.42 Forearm.reconfigure :tight @14:28:57.47 Forearm.seek 5 + 450 counts @14:28:58.49 SE.reconfigure :pick @14:28:58.55 SE.seek :beamBreak @14:28:59.84 SE.configure :default @14:29:00.00 Elbow.reconfigure :limp @14:29:00.05 Forearm.reconfigure :loose @14:29:00.09 Forearm.seek 5 @14:29:00.35 Elbow.hold @14:29:00.42 Elbow.reconfigure :tight @14:29:00.52 SE.seek 28.13 Pucks below Top Plate @14:29:01.09 Forearm.force -20 @14:29:01.13 Forearm.seek 5 + 314 counts @14:29:01.41 Forearm.reconfigure :tight @14:29:01.46 Forearm.seek 5 + 450 counts @14:29:02.08 SE.seek 29.13 Pucks below Top Plate @14:29:02.66 Forearm.reconfigure :loose @14:29:02.71 Forearm.seek :retract @14:29:03.70 Elbow.seek CC @14:29:05.38 Elbow.reconfigure :loose @14:29:05.49 Forearm.seek CC @14:29:06.46 Hand.close @14:29:07.03 CC.seek :open @14:29:11.77 Forearm.seek :retract @14:29:12.72 Elbow.reconfigure :tight @14:29:12.76 Elbow.seek 5 @14:29:14.53 Elbow.reconfigure :limp @14:29:14.58 Forearm.seek 5 @14:29:15.56 Elbow.hold @14:29:15.62 Elbow.reconfigure :tight @14:29:15.67 Hand.open @14:29:16.40 Forearm.force -20 @14:29:16.44 Forearm.seek 5 + 284 counts @14:29:16.73 Forearm.reconfigure :tight @14:29:16.78 Forearm.seek 5 + 450 counts @14:29:17.14 SE.seek :down @14:29:17.86 SE.stop @14:29:18.27
Forearm.reconfigure :loose @14:29:18.31 Forearm.seek :retract @14:29:19.23 PSV.dial :air @14:29:19.98 PS.seek 2.5ml @14:29:46.38 delay 1 second @14:29:47.39 PSV.dial :mixloop @14:29:47.85 delay 1 second @14:29:48.86 PSV.dial :air @14:29:49.46 delay 1 second @14:29:50.47 PSV.dial :mixloop @14:29:50.93 delay 1 second @14:29:51.94 PSV.dial :air @14:29:52.41 delay 1 second @14:29:53.43 PS.seek 0ml @14:30:19.75 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @14:30:19.93 Elbow.seek FlushPuck::Garage @14:30:21.77 Elbow.reconfigure :loose @14:30:21.87 Forearm.seek :garage @14:30:22.90 Hand.close @14:30:23.64 Forearm.seek :retract @14:30:24.62 Elbow.reconfigure :tight @14:30:24.67 Elbow.seek PC @14:30:25.96 Elbow.reconfigure :loose @14:30:26.06 Forearm.reconfigure :tight @14:30:26.10 Forearm.seek PC @14:30:27.31 PC.seek :closed @14:30:31.95 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.65 @14:30:32.01 Hand.open @14:30:32.71 Forearm.reconfigure :loose @14:30:32.76 Forearm.seek :retract @14:30:33.71 PRV.dial :air @14:30:34.17 PSV.dial :mixloop @14:30:34.77 PS.seek 0.3ml @14:30:38.62 PSV.dial PSR @14:30:39.69 Pulling 0.4ml of PSR.air @14:30:39.70 PSR.select :air @14:30:39.72 PS.seek 0.7ml @14:30:44.59 delay 3 seconds @14:30:47.61 PSR.select :air @14:30:47.64 PS.seek 2.5ml @14:31:06.78 delay 3 seconds @14:31:09.80 PSV.dial :mixloop @14:31:10.86 PTV.dial :mixing @14:31:11.63 PBV.dial :waste2 @14:31:12.58 PS.seek 0ml @14:31:39.00 Garaging FlushPuck in Processing Clamp @14:31:39.07 Elbow.reconfigure :tight @14:31:39.12 Elbow.seek PC @14:31:39.24 Elbow.reconfigure :loose @14:31:39.34 Forearm.seek PC @14:31:40.32 Hand.close @14:31:40.90 PC.seek :open @14:31:45.61 Forearm.seek :retract @14:31:46.62 Elbow.reconfigure :tight @14:31:46.67 Elbow.seek :garage @14:31:47.99 Elbow.reconfigure :loose @14:31:48.09 Forearm.reconfigure :tight @14:31:48.13 Forearm.seek :garage @14:31:49.20 Hand.open @14:31:49.83 Forearm.reconfigure :loose @14:31:49.88 Forearm.seek :retract @14:31:50.85 Loading da puck from tube 6 into Processing Clamp @14:31:51.08 SC.reconfigure :tight @14:31:51.12 SC.seek 6 @14:31:54.71 SC.configure :limp @14:31:54.91 Elbow.reconfigure :tight @14:31:54.96 Elbow.seek 6 @14:31:57.30 Forearm.reconfigure :tight @14:31:57.37 Forearm.seek 6 + 450 counts @14:31:58.40 SE.reconfigure :pick @14:31:58.46 SE.seek :beamBreak @14:32:01.40 SE.configure :default @14:32:01.56 Elbow.seek 6 @14:32:01.74 Forearm.seek 6 + 300 counts @14:32:01.88 Forearm.reconfigure :loose @14:32:01.93 Hand.close @14:32:02.54 SE.seek :down @14:32:02.73
Forearm.seek :retract @14:32:03.70 Elbow.seek PC @14:32:05.17 SE.stop @14:32:06.29
Elbow.reconfigure :loose @14:32:06.39 Forearm.reconfigure :tight @14:32:06.43 Forearm.seek PC @14:32:07.62 PC.seek :closed @14:32:12.26 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.66 @14:32:12.27 Hand.open @14:32:12.96 Forearm.reconfigure :loose @14:32:13.00 Forearm.seek :retract @14:32:13.94 Heating Puck.from 6, :da in Processing Clamp to 28C for 2:00:00 @14:32:13.96 PH.seek 28C,5:00 @14:32:56.73 PH.hold 2:00:00,28C @14:32:56.78 delay 1:30 @14:34:26.82 PSR.select :air @14:34:26.84 PSV.dial PSR @14:34:27.90 Pulling 0.5ml of PSR.wash @14:34:27.91 PSR.select :wash @14:34:28.03 PS.seek 0.5ml @14:34:34.00 delay 5 seconds @14:34:39.02 PSR.select :air @14:34:39.14 PS.seek 2ml @14:34:55.34 PTV.dial :puck @14:34:56.09 PSV.dial PTV @14:34:56.85 PBV.dial :waste1 @14:34:58.26 PS.seek 1.5ml @14:35:04.25 delay 3 seconds @14:35:07.26 PBV.dial between PSV and waste1 @14:35:07.61 PS.seek 0ml @14:35:23.78 delay 5:00 @14:40:23.80 recoverPSRreagent08 PTV.dial :puck @14:40:23.88 PBV.dial :waste1 @14:40:24.61 delay 30 seconds @14:40:54.71 PBV.dial between PSV and waste1 @14:40:55.07 PSR.select :air @14:40:55.08 PSV.dial PSR @14:40:55.86 PS.reconfigure :fast @14:40:55.90 PS.seek 2.5ml @14:41:19.52 PSV.dial PTV @14:41:20.29 delay 5 seconds @14:41:25.31 PSV.dial PSR @14:41:26.05 delay 5 seconds @14:41:31.07 PSV.dial PTV @14:41:31.94 delay 5 seconds @14:41:36.96 PSV.dial :air @14:41:37.85 PS.seek 0ml @14:42:01.15 PTV.dial :PRVmixing,via: PRV @14:42:02.47 PSV.dial :mixloop @14:42:02.93 PRV.dial :ab1 @14:42:03.67 PS.reconfigure :slow @14:42:03.71 PS.seek 0.15ml @14:42:07.33 delay 2 seconds @14:42:09.35 CBV.dial PRV @14:42:09.37
PRV.dial CBV @14:42:09.94 PS.seek 0.35ml @14:42:14.61 delay 2 seconds @14:42:16.63 CBV.dial between PRV and PSV @14:42:16.96 PRV.dial :air @14:42:17.42 PS.seek 0.9ml @14:42:29.17 PRV.dial :waste2 @14:42:29.93 PS.seek 0.5ml @14:42:38.68 addPMLReagent08 PSV.dial :air @14:42:39.15 PS.reconfigure :default @14:42:39.20 PS.seek 2ml @14:42:55.33 PSV.dial :PML @14:42:55.80 PTV.dial :mixing @14:42:56.56 PBV.dial :waste1 @14:42:57.00 PS.seek 1.85ml @14:42:59.33 PBV.dial between PSV and waste1 @14:42:59.71 PS.seek 0ml @14:43:19.39 delay 2 seconds @14:43:21.41 Beginning samplePassC @14:43:21.43 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:43:21.60 Elbow.reconfigure :tight @14:43:21.65 Elbow.seek FlushPuck::Garage @14:43:23.09 Elbow.reconfigure :loose @14:43:23.19 Forearm.seek :garage @14:43:24.62 Hand.close @14:43:25.39 Forearm.seek :retract @14:43:26.35 Elbow.reconfigure :tight @14:43:26.40 Elbow.seek CC @14:43:29.36 Elbow.reconfigure :loose @14:43:29.46 Forearm.reconfigure :tight @14:43:29.51 Forearm.seek CC @14:43:30.73 CC.seek :closed @14:43:35.24 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.48 @14:43:35.31 Hand.open @14:43:35.95 Forearm.reconfigure :loose @14:43:36.00 Forearm.seek :retract @14:43:36.95 PSV.dial PSR @14:43:38.03 Pulling 1.5ml of PSR.flush @14:43:38.04 PSR.select :flush @14:43:38.16 PS.seek 1.5ml @14:43:54.30 delay 5 seconds @14:43:59.32 PSV.dial :waste1 @14:43:59.81 PS.seek 0ml @14:44:15.89 PSV.dial PSR @14:44:16.36 PSR.select :air @14:44:16.48 PS.seek 2.5ml @14:44:42.84 PRV.dial CBV,avoiding: :ab2 @14:44:42.86
CBV.dial PRV @14:44:44.49 PSV.dial :mixloop @14:44:45.53 CTV.dial :air,avoiding: :intake @14:44:45.61 PTV.dial :PRVmixing @14:44:46.38 PS.seek 0ml @14:45:12.80 PSV.dial :air @14:45:13.27 PS.seek 2.5ml @14:45:39.70 PSV.dial :mixloop @14:45:40.19 PS.seek 0ml @14:46:06.51 PSV.dial :air @14:46:07.12 PS.seek 2.5ml @14:46:33.42 PSV.dial :mixloop @14:46:33.88 PS.seek 0ml @14:47:00.42 Garaging FlushPuck in Collection Clamp @14:47:00.49 Elbow.reconfigure :tight @14:47:00.54 Elbow.seek CC @14:47:00.84 Elbow.reconfigure :loose @14:47:00.94 Forearm.seek CC @14:47:01.89 Hand.close @14:47:02.52 CC.seek :open @14:47:07.16 Forearm.seek :retract @14:47:08.13 Elbow.reconfigure :tight @14:47:08.17 Elbow.seek :garage @14:47:11.07 Elbow.reconfigure :loose @14:47:11.17 Forearm.reconfigure :tight @14:47:11.22 Forearm.seek :garage @14:47:12.16 Hand.open @14:47:12.82 Forearm.reconfigure :loose @14:47:12.87 Forearm.seek :retract @14:47:13.89 delayUntil "14:51:21.40" @14:47:13.92 Whole Cell Archive sampling 1000ml @14:47:13.94 Loading wcr puck from tube 6 into Collection Clamp @14:47:14.18 SC.reconfigure :tight @14:47:14.23 SC.seek 6 @14:47:14.29 SC.configure :limp @14:47:14.51 Elbow.reconfigure :tight @14:47:14.56 Elbow.seek 6 @14:47:16.95 Forearm.reconfigure :tight @14:47:17.00 Forearm.seek 6 + 450 counts @14:47:18.09 SE.reconfigure :pick @14:47:18.15 SE.seek :beamBreak @14:47:21.22 SE.configure :default @14:47:21.38 Elbow.seek 6 @14:47:21.57 Forearm.seek 6 + 300 counts @14:47:21.72 Forearm.reconfigure :loose @14:47:21.76 Hand.close @14:47:22.40 SE.seek :down @14:47:22.61 Forearm.seek :retract @14:47:23.57 Elbow.seek CC @14:47:25.10 SE.stop @14:47:25.22 Elbow.reconfigure :loose @14:47:25.32 Forearm.reconfigure :tight @14:47:25.37 Forearm.seek CC @14:47:26.59 CC.seek :closed @14:47:31.12 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.24 @14:47:31.13 Hand.open @14:47:31.82 Forearm.reconfigure :loose @14:47:31.87 Forearm.seek :retract @14:47:33.30 collectSample 1000ml w/bubble point of 35psi @14:47:33.31 Priming 7ml @14:47:33.32 Intake.select :close @14:47:33.33 Exhaust.select :close @14:47:33.35 SS.hold @14:47:33.56 Calibrating pressure sensors @14:47:33.57 CTV.dial :air @14:47:33.66 SSV.dial :spit,avoiding: :exhaust @14:47:33.75 SS.reconfigure :fast @14:47:33.80 SS.seek 5ml @14:47:36.49 SSV.dial CBV @14:47:36.52 CBV.dial SSV @14:47:37.87 delay 3.8 seconds @14:47:41.80 Sensor limits: Intake max 100.9psia; Outlet max 92.7psia @14:47:41.81 SSV.dial :spit @14:47:43.17 SS.seek 0ml @14:47:46.05 SSV.dial CBV @14:47:47.40 CTV.dial :intake @14:47:48.17 SSV.dial CBV @14:47:48.19 CBV.dial SSV @14:47:48.34 Allowing 40s for 7.0ml of remaining 7.0ml @14:47:48.36 Intake.select :open @14:47:50.52 Intake @14.7psia, vacuum from 9.2 to 12.2psi @14:47:50.54 SS.reconfigure :inhaling 2.20ml/s @14:47:50.59 SS.seek 7ml @14:47:57.61 Stroke completed -- waiting for pressure to equalize ... @14:47:57.63 SS.reconfigure :exhale @14:47:57.67 SS.hold @14:48:18.52 Intake.select :close @14:48:18.68 SSV.dial :exhaust @14:48:19.45 Exhaust.select :open @14:48:19.57 SS.seek 0ml @14:48:23.64 Exhaust.select :close @14:48:23.76 Sampling 1000.0ml @14:48:23.77 Intake.select :close @14:48:23.78 Exhaust.select :close @14:48:23.79 SS.reconfigure :default @14:48:23.84 SS.hold @14:48:24.04 CTV.dial :intake @14:48:24.12 SSV.dial CBV @14:48:24.16 CBV.dial SSV @14:48:24.93 Allowing 130s for 25.0ml of remaining 1000.0ml @14:48:24.94 Intake.select :open @14:48:27.11 Intake @15.5psia, vacuum from 10.0 to 13.0psi @14:48:27.13 SS.reconfigure :inhaling 2.20ml/s @14:48:27.18 SS.seek 25ml @14:48:34.81 Vacuum too high -- waiting up to 40 seconds @14:48:34.82 SS.reconfigure :exhale @14:48:34.88 SS.hold @14:48:44.04 Intake @19.1psia, vacuum from 10.0 to 13.0psi @14:48:44.06 SS.reconfigure :inhaling 1.76ml/s @14:48:44.11 SS.seek 25ml @14:48:46.22 Vacuum too high -- waiting up to 40 seconds @14:48:46.23 SS.reconfigure :exhale @14:48:46.29 SS.hold @14:48:49.00 Intake @19.0psia, vacuum from 10.0 to 13.0psi @14:48:49.02 SS.reconfigure :inhaling 1.41ml/s @14:48:49.07 SS.seek 25ml @14:48:51.44 Vacuum too high -- waiting up to 40 seconds @14:48:51.46 SS.reconfigure :exhale @14:48:51.51 SS.hold @14:48:53.91 Intake @19.2psia, vacuum from 10.0 to 13.0psi @14:48:53.92 SS.reconfigure :inhaling 1.13ml/s @14:48:53.98 SS.seek 25ml @14:48:56.43 Vacuum too high -- waiting up to 40 seconds @14:48:56.46 SS.reconfigure :exhale @14:48:56.51 SS.hold @14:48:58.64 Intake @19.5psia, vacuum from 10.0 to 13.0psi @14:48:58.66 SS.reconfigure :inhaling 0.91ml/s @14:48:58.70 SS.seek 25ml @14:49:01.60 Vacuum too high -- waiting up to 40 seconds @14:49:01.62 SS.reconfigure :exhale @14:49:01.67 SS.hold @14:49:03.79 Intake @19.1psia, vacuum from 10.0 to 13.0psi @14:49:03.81 SS.reconfigure :inhaling 0.73ml/s @14:49:03.86 SS.seek 25ml @14:49:08.31 Vacuum too high -- waiting up to 40 seconds @14:49:08.32 SS.reconfigure :exhale @14:49:08.38 SS.hold @14:49:10.33 Intake @19.2psia, vacuum from 10.0 to 13.0psi @14:49:10.35 SS.reconfigure :inhaling 0.59ml/s @14:49:10.39 SS.seek 25ml @14:49:15.28 Stroke completed -- waiting for pressure to equalize ... @14:49:15.30 SS.reconfigure :exhale @14:49:15.34 SS.hold @14:49:48.10 Intake.select :close @14:49:48.26 SSV.dial :exhaust @14:49:49.03 Exhaust.select :open @14:49:49.14 SS.seek 0ml @14:49:58.16 Exhaust.select :close @14:49:58.27 SSV.dial CBV @14:49:58.29 CBV.dial SSV @14:49:59.07 Allowing 130s for 25.0ml of remaining 975.0ml @14:49:59.08 Intake.select :open @14:50:01.25 Intake @16.1psia, vacuum from 10.0 to 13.0psi @14:50:01.27 SS.reconfigure :inhaling 0.59ml/s @14:50:01.31 SS.seek 25ml @14:50:45.70 Stroke completed -- waiting for pressure to equalize ... @14:50:45.72 SS.reconfigure :exhale @14:50:45.76 SS.hold @14:51:21.41 recoverPRVreagent08 @14:51:21.42 PTV.dial :mixing @14:51:22.18 PBV.dial :waste1 @14:51:22.44 Intake.select :close @14:51:22.56 delay 30 seconds @14:51:22.64 SSV.dial :exhaust @14:51:23.41 Exhaust.select :open @14:51:23.52 SS.seek 0ml @14:51:32.36 Exhaust.select :close @14:51:32.48 SSV.dial CBV @14:51:32.50 CBV.dial SSV @14:51:33.30 Allowing 130s for 25.0ml of remaining 950.0ml @14:51:33.31 Intake.select :open @14:51:35.48 Intake @16.0psia, vacuum from 10.0 to 13.0psi @14:51:35.50 SS.reconfigure :inhaling 0.59ml/s @14:51:35.55 SS.seek 25ml @14:51:52.58 PBV.dial between PSV and waste1 @14:51:52.95 PSV.dial :air @14:51:53.42 PS.reconfigure :fast @14:51:53.92 PS.seek 2.5ml @14:52:17.82 PSV.dial :mixloop @14:52:18.29 delay 2 seconds @14:52:20.31 PSV.dial :air @14:52:20.59 Stroke completed -- waiting for pressure to equalize ... @14:52:20.60 SS.reconfigure :exhale @14:52:20.65 SS.hold @14:52:20.78 delay 2 seconds @14:52:22.79 PSV.dial :mixloop @14:52:23.26 delay 2 seconds @14:52:25.27 PSV.dial :air @14:52:25.75 PS.seek 0ml @14:52:48.06 Intake.select :close @14:52:48.22 SSV.dial :exhaust @14:52:49.00 Exhaust.select :open @14:52:49.12 SS.seek 0ml @14:52:49.26 washCycleDA #1 of 2 @14:52:49.27 PSR.select :air @14:52:49.29 PSV.dial PSR @14:52:50.63 Pulling 0.5ml of PSR.wash @14:52:50.64 PSR.select :wash @14:52:50.76 PS.reconfigure :default @14:52:50.81 PS.seek 0.5ml @14:52:56.69 delay 5 seconds @14:52:57.92 Exhaust.select :close @14:52:58.04 SSV.dial CBV @14:52:58.06 CBV.dial SSV @14:52:58.84 Allowing 130s for 25.0ml of remaining 925.0ml @14:52:58.86 Intake.select :open @14:53:01.02 Intake @16.3psia, vacuum from 10.0 to 13.0psi @14:53:01.04 SS.reconfigure :inhaling 0.59ml/s @14:53:01.09 SS.seek 25ml @14:53:01.71 PSR.select :air @14:53:01.83 PS.seek 2ml @14:53:18.07 PTV.dial :puck @14:53:18.82 PSV.dial PTV @14:53:19.57 PBV.dial :waste1 @14:53:20.00 PS.seek 1.5ml @14:53:25.94 delay 3 seconds @14:53:28.96 PBV.dial between PSV and waste1 @14:53:29.34 PS.seek 0ml @14:53:45.44 delay 2:00 @14:53:45.95 Stroke completed -- waiting for pressure to equalize ... @14:53:45.96 SS.reconfigure :exhale @14:53:46.01 SS.hold @14:54:14.62 Intake.select :close @14:54:14.78 SSV.dial :exhaust @14:54:15.56 Exhaust.select :open @14:54:15.68 SS.seek 0ml @14:54:24.50 Exhaust.select :close @14:54:24.61 SSV.dial CBV @14:54:24.63 CBV.dial SSV @14:54:25.42 Allowing 130s for 25.0ml of remaining 900.0ml @14:54:25.43 Intake.select :open @14:54:27.60 Intake @16.2psia, vacuum from 10.0 to 13.0psi @14:54:27.62 SS.reconfigure :inhaling 0.59ml/s @14:54:27.67 SS.seek 25ml @14:55:12.54 Stroke completed -- waiting for pressure to equalize ... @14:55:12.55 SS.reconfigure :exhale @14:55:12.60 SS.hold @14:55:37.57 Intake.select :close @14:55:37.73 SSV.dial :exhaust @14:55:38.51 Exhaust.select :open @14:55:38.63 SS.seek 0ml @14:55:45.47 recoverPSRreagent08 @14:55:45.48 PTV.dial :puck @14:55:45.99 PBV.dial :waste1 @14:55:46.38 delay 30 seconds @14:55:47.46 Exhaust.select :close @14:55:47.57 SSV.dial CBV @14:55:47.62 CBV.dial SSV @14:55:48.37 Allowing 130s for 25.0ml of remaining 875.0ml @14:55:48.39 Intake.select :open @14:55:50.56 Intake @16.3psia, vacuum from 10.0 to 13.0psi @14:55:50.58 SS.reconfigure :inhaling 0.59ml/s @14:55:50.63 SS.seek 25ml @14:56:16.40 PBV.dial between PSV and waste1 @14:56:16.77 PSR.select :air @14:56:16.79 PSV.dial PSR @14:56:17.56 PS.reconfigure :fast @14:56:17.61 PS.seek 2.5ml @14:56:35.38 Stroke completed -- waiting for pressure to equalize ... @14:56:35.40 SS.reconfigure :exhale @14:56:35.44 SS.hold @14:56:41.50 PSV.dial PTV @14:56:42.27 delay 5 seconds @14:56:47.28 PSV.dial PSR @14:56:48.56 delay 5 seconds @14:56:53.58 PSV.dial PTV @14:56:54.35 delay 5 seconds @14:56:59.36 PSV.dial :air @14:57:00.13 PS.seek 0ml @14:57:02.47 Intake.select :close @14:57:02.63 SSV.dial :exhaust @14:57:03.39 Exhaust.select :open @14:57:03.51 SS.seek 0ml @14:57:12.57 Exhaust.select :close @14:57:12.68 SSV.dial CBV @14:57:12.70 CBV.dial SSV @14:57:13.48 Allowing 130s for 25.0ml of remaining 850.0ml @14:57:13.50 Intake.select :open @14:57:15.66 Intake @16.1psia, vacuum from 10.0 to 13.0psi @14:57:15.68 SS.reconfigure :inhaling 0.59ml/s @14:57:15.73 SS.seek 25ml @14:57:23.82 washCycleDA #2 of 2 @14:57:23.83 PSR.select :air @14:57:23.84 PSV.dial PSR @14:57:25.19 Pulling 0.5ml of PSR.wash @14:57:25.20 PSR.select :wash @14:57:25.31 PS.reconfigure :default @14:57:25.36 PS.seek 0.5ml @14:57:31.28 delay 5 seconds @14:57:36.30 PSR.select :air @14:57:36.42 PS.seek 2ml @14:57:52.53 PTV.dial :puck @14:57:52.61 PSV.dial PTV @14:57:53.49 PBV.dial :waste1 @14:57:53.92 PS.seek 1.5ml @14:57:59.91 delay 3 seconds @14:58:00.21 Stroke completed -- waiting for pressure to equalize ... @14:58:00.22 SS.reconfigure :exhale @14:58:00.27 SS.hold @14:58:02.92 PBV.dial between PSV and waste1 @14:58:03.30 PS.seek 0ml @14:58:19.63 delay 2:00 @14:58:27.30 Intake.select :close @14:58:27.64 SSV.dial :exhaust @14:58:28.41 Exhaust.select :open @14:58:28.52 SS.seek 0ml @14:58:37.91 Exhaust.select :close @14:58:38.09 SSV.dial CBV @14:58:38.12 CBV.dial SSV @14:58:38.88 Allowing 130s for 25.0ml of remaining 825.0ml @14:58:38.90 Intake.select :open @14:58:41.07 Intake @16.1psia, vacuum from 10.0 to 13.0psi @14:58:41.09 SS.reconfigure :inhaling 0.59ml/s @14:58:41.14 SS.seek 25ml @14:59:25.51 Stroke completed -- waiting for pressure to equalize ... @14:59:25.52 SS.reconfigure :exhale @14:59:25.57 SS.hold @14:59:52.18 Intake.select :close @14:59:52.34 SSV.dial :exhaust @14:59:53.11 Exhaust.select :open @14:59:53.23 SS.seek 0ml @15:00:02.22 Exhaust.select :close @15:00:02.33 SSV.dial CBV @15:00:02.37 CBV.dial SSV @15:00:03.13 Allowing 130s for 25.0ml of remaining 800.0ml @15:00:03.15 Intake.select :open @15:00:05.31 Intake @16.2psia, vacuum from 10.0 to 13.0psi @15:00:05.33 SS.reconfigure :inhaling 0.59ml/s @15:00:05.38 SS.seek 25ml @15:00:19.65 recoverPSRreagent08 @15:00:19.66 PTV.dial :puck @15:00:19.73 PBV.dial :waste1 @15:00:20.11 delay 30 seconds @15:00:34.76 Vacuum too high -- waiting up to 40 seconds @15:00:34.78 SS.reconfigure :exhale @15:00:34.83 SS.hold @15:00:42.86 Intake @17.0psia, vacuum from 10.0 to 13.0psi @15:00:42.88 SS.reconfigure :inhaling 0.47ml/s @15:00:42.93 SS.seek 25ml @15:00:50.13 PBV.dial between PSV and waste1 @15:00:50.50 PSR.select :air @15:00:50.52 PSV.dial PSR @15:00:51.27 PS.reconfigure :fast @15:00:51.32 PS.seek 2.5ml @15:01:01.91 Stroke completed -- waiting for pressure to equalize ... @15:01:01.92 SS.reconfigure :exhale @15:01:01.97 SS.hold @15:01:15.43 PSV.dial PTV @15:01:16.20 delay 5 seconds @15:01:21.22 PSV.dial PSR @15:01:22.44 delay 5 seconds @15:01:22.89 Intake.select :close @15:01:23.05 SSV.dial :exhaust @15:01:23.83 Exhaust.select :open @15:01:23.94 SS.seek 0ml @15:01:27.45 PSV.dial PTV @15:01:28.22 delay 5 seconds @15:01:32.72 Exhaust.select :close @15:01:32.83 SSV.dial CBV @15:01:32.85 CBV.dial SSV @15:01:33.23 PSV.dial :air @15:01:33.63 Allowing 130s for 25.0ml of remaining 775.0ml @15:01:33.65 Intake.select :open @15:01:34.13 PS.seek 0ml @15:01:35.81 Intake @16.3psia, vacuum from 10.0 to 13.0psi @15:01:35.83 SS.reconfigure :inhaling 0.47ml/s @15:01:35.88 SS.seek 25ml @15:01:57.50 getPMLReagent08 @15:01:57.51 PRV.dial :air @15:01:57.98 delay 5 seconds @15:02:03.00 PTV.dial :PRVmixing @15:02:04.33 delay 5 seconds @15:02:09.35 PRV.dial :ab2 @15:02:09.79 PTV.dial :PRVmixing @15:02:09.81 PSV.dial :mixloop @15:02:10.27 PS.reconfigure :default @15:02:10.31 PS.seek 0.3ml @15:02:14.25 delay 2 seconds @15:02:16.26 PRV.dial :air @15:02:16.79 PS.seek 0.55ml @15:02:20.12 addPMLReagent08 @15:02:20.13 PSV.dial :air @15:02:20.72 PS.seek 2ml @15:02:31.52 Stroke completed -- waiting for pressure to equalize ... @15:02:31.53 SS.reconfigure :exhale @15:02:31.58 SS.hold @15:02:36.27 PSV.dial :PML @15:02:36.74 PTV.dial :mixing @15:02:37.49 PBV.dial :waste1 @15:02:37.93 PS.seek 1.85ml @15:02:40.34 PBV.dial between PSV and waste1 @15:02:40.72 PS.seek 0ml @15:02:58.13 Intake.select :close @15:02:58.29 SSV.dial :exhaust @15:02:59.06 Exhaust.select :open @15:02:59.17 SS.seek 0ml @15:03:00.46 delay 2 seconds @15:03:02.48 delay 8:00 @15:03:07.94 Exhaust.select :close @15:03:08.05 SSV.dial CBV @15:03:08.10 CBV.dial SSV @15:03:08.85 Allowing 130s for 25.0ml of remaining 750.0ml @15:03:08.86 Intake.select :open @15:03:11.03 Intake @16.2psia, vacuum from 10.0 to 13.0psi @15:03:11.05 SS.reconfigure :inhaling 0.47ml/s @15:03:11.55 SS.seek 25ml @15:04:06.86 Stroke completed -- waiting for pressure to equalize ... @15:04:06.87 SS.reconfigure :exhale @15:04:06.92 SS.hold @15:04:36.00 Intake.select :close @15:04:36.16 SSV.dial :exhaust @15:04:36.93 Exhaust.select :open @15:04:37.05 SS.seek 0ml @15:04:45.88 Exhaust.select :close @15:04:45.99 SSV.dial CBV @15:04:46.01 CBV.dial SSV @15:04:46.78 Allowing 130s for 25.0ml of remaining 725.0ml @15:04:46.80 Intake.select :open @15:04:48.96 Intake @16.2psia, vacuum from 10.0 to 13.0psi @15:04:48.98 SS.reconfigure :inhaling 0.47ml/s @15:04:49.03 SS.seek 25ml @15:05:29.63 Vacuum too high -- waiting up to 40 seconds @15:05:29.65 SS.reconfigure :exhale @15:05:29.70 SS.hold @15:05:40.97 Intake @16.4psia, vacuum from 10.0 to 13.0psi @15:05:40.98 SS.reconfigure :inhaling 0.38ml/s @15:05:41.49 SS.seek 25ml @15:05:59.95 Stroke completed -- waiting for pressure to equalize ... @15:05:59.96 SS.reconfigure :exhale @15:06:00.01 SS.hold @15:06:24.66 Intake.select :close @15:06:24.82 SSV.dial :exhaust @15:06:26.05 Exhaust.select :open @15:06:26.16 SS.seek 0ml @15:06:35.20 Exhaust.select :close @15:06:35.32 SSV.dial CBV @15:06:35.34 CBV.dial SSV @15:06:36.11 Allowing 130s for 25.0ml of remaining 700.0ml @15:06:36.13 Intake.select :open @15:06:38.29 Intake @16.2psia, vacuum from 10.0 to 13.0psi @15:06:38.31 SS.reconfigure :inhaling 0.38ml/s @15:06:38.36 SS.seek 25ml @15:07:46.06 Stroke completed -- waiting for pressure to equalize ... @15:07:46.07 SS.reconfigure :exhale @15:07:46.12 SS.hold @15:08:21.34 Intake.select :close @15:08:21.50 SSV.dial :exhaust @15:08:22.26 Exhaust.select :open @15:08:22.38 SS.seek 0ml @15:08:31.32 Exhaust.select :close @15:08:31.43 SSV.dial CBV @15:08:31.45 CBV.dial SSV @15:08:32.23 Allowing 130s for 25.0ml of remaining 675.0ml @15:08:32.25 Intake.select :open @15:08:34.41 Intake @16.2psia, vacuum from 10.0 to 13.0psi @15:08:34.43 SS.reconfigure :inhaling 0.38ml/s @15:08:34.48 SS.seek 25ml @15:09:21.16 Vacuum too high -- waiting up to 40 seconds @15:09:21.17 SS.reconfigure :exhale @15:09:21.23 SS.hold @15:09:35.70 Intake @17.4psia, vacuum from 10.0 to 13.0psi @15:09:35.71 SS.reconfigure :inhaling 0.31ml/s @15:09:35.76 SS.seek 25ml @15:10:02.90 Stroke completed -- waiting for pressure to equalize ... @15:10:02.91 SS.reconfigure :exhale @15:10:02.96 SS.hold @15:10:29.98 Intake.select :close @15:10:30.14 SSV.dial :exhaust @15:10:30.90 Exhaust.select :open @15:10:31.02 SS.seek 0ml @15:10:39.90 Exhaust.select :close @15:10:40.01 SSV.dial CBV @15:10:40.05 CBV.dial SSV @15:10:40.82 Allowing 130s for 25.0ml of remaining 650.0ml @15:10:40.84 Intake.select :open @15:10:43.00 Intake @16.2psia, vacuum from 10.0 to 13.0psi @15:10:43.02 SS.reconfigure :inhaling 0.31ml/s @15:10:43.07 SS.seek 25ml @15:11:02.50 recoverPRVreagent08 @15:11:02.52 PTV.dial :mixing @15:11:02.60 PBV.dial :waste1 @15:11:02.97 delay 30 seconds @15:11:33.00 PBV.dial between PSV and waste1 @15:11:33.36 PSV.dial :air @15:11:33.84 PS.reconfigure :fast @15:11:33.88 PS.seek 2.5ml @15:11:58.17 PSV.dial :mixloop @15:11:58.66 delay 2 seconds @15:12:00.68 PSV.dial :air @15:12:01.14 delay 2 seconds @15:12:03.16 PSV.dial :mixloop @15:12:03.63 delay 2 seconds @15:12:05.64 PSV.dial :air @15:12:06.12 PS.seek 0ml @15:12:10.74 Stroke completed -- waiting for pressure to equalize ... @15:12:10.75 SS.reconfigure :exhale @15:12:10.80 SS.hold @15:12:29.58 washCycleDA #1 of 3 @15:12:29.59 PSR.select :air @15:12:29.61 PSV.dial PSR @15:12:30.94 Pulling 0.5ml of PSR.wash @15:12:30.95 PSR.select :wash @15:12:31.06 PS.reconfigure :default @15:12:31.11 PS.seek 0.5ml @15:12:37.01 delay 5 seconds @15:12:42.15 PSR.select :air @15:12:42.33 PS.seek 2ml @15:12:49.93 Intake.select :close @15:12:50.09 SSV.dial :exhaust @15:12:50.87 Exhaust.select :open @15:12:50.99 SS.seek 0ml @15:12:58.42 PTV.dial :puck @15:12:59.17 PSV.dial PTV @15:12:59.82 Exhaust.select :close @15:12:59.93 SSV.dial CBV @15:12:59.96 CBV.dial SSV @15:13:00.05 PBV.dial :waste1 @15:13:00.48 PS.seek 1.5ml @15:13:00.73 Allowing 130s for 25.0ml of remaining 625.0ml @15:13:00.74 Intake.select :open @15:13:02.91 Intake @16.1psia, vacuum from 10.0 to 13.0psi @15:13:02.93 SS.reconfigure :inhaling 0.31ml/s @15:13:02.98 SS.seek 25ml @15:13:06.42 delay 3 seconds @15:13:09.44 PBV.dial between PSV and waste1 @15:13:09.82 PS.seek 0ml @15:13:25.99 delay 2:00 @15:14:15.44 Vacuum too high -- waiting up to 40 seconds @15:14:15.45 SS.reconfigure :exhale @15:14:15.50 SS.hold @15:14:38.06 Intake @17.6psia, vacuum from 10.0 to 13.0psi @15:14:38.08 SS.reconfigure :inhaling 0.25ml/s @15:14:38.13 SS.seek 25ml @15:14:57.55 Stroke completed -- waiting for pressure to equalize ... @15:14:57.56 SS.reconfigure :exhale @15:14:57.61 SS.hold @15:15:10.79 SS.reconfigure :default @15:15:10.84 SS.hold @15:15:10.91 Intake.select :close @15:15:11.07 ShallowSampler::Clogged in WCR -- Filtering too Slowly after 400.0ml @15:15:11.08 SSV.dial :exhaust @15:15:11.86 Exhaust.select :open @15:15:11.98 SS.reconfigure :exhale @15:15:12.03 SS.seek 0ml @15:15:21.08 Exhaust.select :close @15:15:21.20 Sampled 400.0ml @15:15:21.22 Drying puck before fixing for 5:00 CBV.dial :waste2b @15:15:21.99 delay 1:00 @15:15:26.01 recoverPSRreagent08 @15:15:26.03 PTV.dial :puck @15:15:26.11 PBV.dial :waste1 @15:15:26.48 delay 30 seconds @15:15:56.51 PBV.dial between PSV and waste1 @15:15:56.89 PSR.select :air @15:15:56.91 PSV.dial PSR @15:15:57.78 PS.reconfigure :fast @15:15:57.83 PS.seek 2.5ml @15:16:21.67 PSV.dial PTV @15:16:22.01 CSV.dial :air @15:16:22.42 delay 5 seconds @15:16:22.47 CS.reconfigure :fast @15:16:22.52 CS.seek 10ml @15:16:27.45 PSV.dial PSR @15:16:28.22 delay 5 seconds @15:16:33.24 PSV.dial PTV @15:16:34.00 delay 5 seconds @15:16:39.02 PSV.dial :air @15:16:39.78 PS.seek 0ml @15:16:46.86 Pumping wcr puck from tube 6 up to 35psi... @15:16:46.88 CBV.dial SSV @15:16:48.15 CBV.dial between CSV and waste2b @15:16:49.12 CSV.dial between air and CTV @15:16:49.92 CS.seek 7.125ml @15:16:57.34 CTV.dial CSV,avoiding: :air @15:16:57.36 CSV.dial CTV @15:16:58.18 CS.reconfigure :pressurize @15:16:58.23 CS.seek 0ml @15:17:03.39 washCycleDA #2 of 3 @15:17:03.40 PSR.select :air @15:17:03.42 PSV.dial PSR @15:17:04.75 Pulling 0.5ml of PSR.wash @15:17:04.76 PSR.select :wash @15:17:04.87 PS.reconfigure :default @15:17:04.92 PS.seek 0.5ml @15:17:10.85 delay 5 seconds @15:17:15.88 PSR.select :air @15:17:15.99 PS.seek 2ml @15:17:16.95 CSV.dial :air @15:17:17.40 CS.reconfigure :fast @15:17:17.45 CS.seek 10ml @15:17:32.20 PTV.dial :puck @15:17:32.28 PSV.dial PTV @15:17:33.03 PBV.dial :waste1 @15:17:33.46 PS.seek 1.5ml @15:17:39.51 delay 3 seconds @15:17:41.85 CSV.dial between air and CTV @15:17:42.18 CS.seek 4.762ml @15:17:42.53 PBV.dial between PSV and waste1 @15:17:42.91 PS.seek 0ml @15:17:54.84 CTV.dial CSV,avoiding: :air @15:17:54.88 CSV.dial CTV @15:17:55.22 CS.reconfigure :pressurize @15:17:55.27 CS.seek 0ml @15:17:59.26 delay 2:00 @15:18:08.08 CSV.dial :air @15:18:08.54 CS.reconfigure :fast @15:18:08.59 CS.seek 10ml @15:18:32.95 CSV.dial between air and CTV @15:18:33.27 CS.seek 4.917ml @15:18:45.83 CTV.dial CSV,avoiding: :air @15:18:45.85 CSV.dial CTV @15:18:46.48 CS.reconfigure :pressurize @15:18:46.58 CS.seek 0ml @15:18:59.73 CSV.dial :air @15:19:00.18 CS.reconfigure :fast @15:19:00.23 CS.seek 10ml @15:19:24.48 CSV.dial between air and CTV @15:19:24.80 CS.seek 4.925ml @15:19:37.05 CTV.dial CSV,avoiding: :air @15:19:37.07 CSV.dial CTV @15:19:37.43 CS.reconfigure :pressurize @15:19:37.48 CS.seek 0ml @15:19:50.67 CSV.dial :air @15:19:51.12 CS.reconfigure :default @15:19:51.17 CS.hold @15:19:51.30 ShallowSampler::Error in MAIN -- Puck.from 6, :wcr reached only 17.5 of 35psi after pumping in 40ml of gas @15:19:51.32 Waiting to Resume from Checkpoint "Pumping wcr puck from tube 6 up to 35psi..." @15:19:51.33 BadNews.email "Puck.from 6, :wcr reached only 17.5 of 35psi after pumping in 40ml of gas",Subject: "ShallowSampler::Error in MAIN" @15:19:56.08 upsync @15:19:59.29 recoverPSRreagent08 @15:19:59.30 PTV.dial :puck @15:19:59.39 PBV.dial :waste1 @15:19:59.76 delay 30 seconds @15:20:29.79 PBV.dial between PSV and waste1 @15:20:30.16 PSR.select :air @15:20:30.19 PSV.dial PSR @15:20:30.95 PS.reconfigure :fast @15:20:31.01 PS.seek 2.5ml @15:20:54.85 PSV.dial PTV @15:20:55.62 delay 5 seconds @15:21:00.64 PSV.dial PSR @15:21:01.38 delay 5 seconds @15:21:06.40 PSV.dial PTV @15:21:07.17 delay 5 seconds @15:21:12.19 PSV.dial :air @15:21:12.95 PS.seek 0ml @15:21:36.29 washCycleDA #3 of 3 @15:21:36.30 PSR.select :air @15:21:36.32 PSV.dial PSR @15:21:37.65 Pulling 0.5ml of PSR.wash @15:21:37.66 PSR.select :wash @15:21:37.78 PS.reconfigure :default @15:21:38.33 PS.seek 0.5ml @15:21:44.48 delay 5 seconds @15:21:49.50 PSR.select :air @15:21:49.61 PS.seek 2ml @15:22:05.75 PTV.dial :puck @15:22:05.83 PSV.dial PTV @15:22:06.58 PBV.dial :waste1 @15:22:07.02 PS.seek 1.5ml @15:22:12.91 delay 3 seconds @15:22:15.93 PBV.dial between PSV and waste1 @15:22:16.31 PS.seek 0ml @15:22:32.41 delay 2:00 @15:24:32.44 recoverPSRreagent08 @15:24:32.45 PTV.dial :puck @15:24:32.52 PBV.dial :waste1 @15:24:32.89 delay 30 seconds @15:25:02.92 PBV.dial between PSV and waste1 @15:25:03.28 PSR.select :air @15:25:03.30 PSV.dial PSR @15:25:04.07 PS.reconfigure :fast @15:25:04.12 PS.seek 2.5ml @15:25:27.88 PSV.dial PTV @15:25:28.64 delay 5 seconds @15:25:33.67 PSV.dial PSR @15:25:34.44 delay 5 seconds @15:25:39.46 PSV.dial PTV @15:25:40.22 delay 5 seconds @15:25:45.24 PSV.dial :air @15:25:46.01 PS.seek 0ml @15:26:09.31 Gate.power :camera,:ON @15:26:09.33 Mixing DA Substrates @15:26:09.34 PSR.select :air @15:26:09.35 PSV.dial PSR @15:26:10.76 Pulling 0.25ml of PSR.sub1 @15:26:10.78 PSR.select :sub1 @15:26:11.00 PS.reconfigure :default @15:26:11.11 PS.seek 0.25ml @15:26:14.57 delay 5 seconds @15:26:19.59 Pulling 0.25ml of PSR.sub2 @15:26:19.61 PSR.select :sub2 @15:26:19.73 PS.seek 0.5ml @15:26:23.28 delay 5 seconds @15:26:28.30 PSR.select :air @15:26:28.42 PS.seek 2ml @15:26:44.59 PTV.dial :puck @15:26:44.67 PSV.dial PTV @15:26:45.44 PBV.dial :waste1 @15:26:45.87 PS.seek 1.5ml @15:26:51.75 delay 3 seconds @15:26:54.77 PBV.dial between PSV and waste1 @15:26:55.15 PS.seek 0ml @15:27:11.28 delay 10 seconds @15:27:21.31 recoverPSRreagent08 @15:27:21.32 PTV.dial :puck @15:27:21.39 PBV.dial :waste1 @15:27:21.77 delay 30 seconds @15:27:51.79 PBV.dial between PSV and waste1 @15:27:52.17 PSR.select :air @15:27:52.19 PSV.dial PSR @15:27:52.97 PS.reconfigure :fast @15:27:53.02 PS.seek 2.5ml @15:28:17.18 PSV.dial PTV @15:28:18.14 delay 5 seconds @15:28:23.16 PSV.dial PSR @15:28:23.92 delay 5 seconds @15:28:28.94 PSV.dial PTV @15:28:29.72 delay 5 seconds @15:28:34.74 PSV.dial :air @15:28:35.52 PS.seek 0ml @15:28:59.31 Additional air clear @15:28:59.32 PSR.select :air @15:28:59.34 PSV.dial PSR @15:29:00.70 Pulling 2ml of PSR.air @15:29:00.71 PSR.select :air @15:29:00.73 PS.reconfigure :default @15:29:00.78 PS.seek 2ml @15:29:21.97 delay 5 seconds @15:29:26.99 PTV.dial :puck @15:29:27.06 PSV.dial PTV @15:29:27.95 PBV.dial :waste1 @15:29:28.38 PS.seek 1.5ml @15:29:34.37 delay 3 seconds @15:29:37.39 PBV.dial between PSV and waste1 @15:29:37.76 delay 1 second @15:29:38.78 PSV.dial :waste1 @15:29:39.87 PS.seek 0ml @15:29:56.05 Processing Puck Heater at 28C before imaging @15:29:56.06 PH.coast @15:29:56.17 Imaging da puck in Processing Clamp while storing it to tube 5 @15:29:56.34 SC.reconfigure :tight @15:29:56.39 SC.seek 5 @15:29:59.94 SC.configure :limp @15:30:00.16 Elbow.reconfigure :tight @15:30:00.21 Elbow.seek 5 @15:30:01.98 Forearm.reconfigure :tight @15:30:02.03 Forearm.seek 5 + 450 counts @15:30:03.06 SE.reconfigure :pick @15:30:03.12 SE.seek :beamBreak @15:30:04.26 SE.configure :default @15:30:04.42 Elbow.reconfigure :limp @15:30:04.47 Forearm.reconfigure :loose @15:30:04.51 Forearm.seek 5 @15:30:04.76 Elbow.hold @15:30:04.82 Elbow.reconfigure :tight @15:30:04.92 SE.seek 29.13 Pucks below Top Plate @15:30:05.44 Forearm.force -20 @15:30:05.49 Forearm.seek 5 + 305 counts @15:30:06.27 Forearm.reconfigure :tight @15:30:06.32 Forearm.seek 5 + 450 counts @15:30:06.53 SE.seek 30.13 Pucks below Top Plate @15:30:07.10 Forearm.reconfigure :loose @15:30:07.15 Forearm.seek :retract @15:30:08.08 Elbow.seek PC @15:30:10.80 Elbow.reconfigure :loose @15:30:10.90 Forearm.seek PC @15:30:11.87 Hand.close @15:30:12.50 PC.seek :open @15:30:17.29 Forearm.seek :retract @15:30:18.22 Elbow.reconfigure :tight @15:30:18.26 Elbow.seek 5 @15:30:20.86 Forearm.reconfigure :tight @15:30:20.91 Forearm.seek 5 + 450 counts @15:30:21.93 Hand.open @15:30:22.67 SE.seek 29.78 Pucks below Top Plate @15:30:22.95 SE.reconfigure :seal @15:30:23.00 SE.seek 28.82 Pucks below Top Plate @15:30:23.53 Lights, camera, ACTION! @15:30:23.57 PuckCamera: snap -bin=2,2 -auto /var/log/esp/da23dec1415h625ml.tif @15:30:43.05 Waiting >20 seconds for da to relinquish FlushPuck @15:30:45.07 SX HX9/16 (USB:28): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 18.203 seconds /var/log/esp/da23dec1415h625ml.tif: TIFF Upload Complete @15:30:45.09 PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da23dec1415h625ml15s.tif @15:31:00.71 SX HX9/16 (USB:28): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15 seconds /var/log/esp/midres/da23dec1415h625ml15s.tif: TIFF Upload Complete @15:31:00.73 PuckCamera: snap -bin=2,2 20.0 /var/log/esp/midres/da23dec1415h625ml20s.tif @15:31:21.35 SX HX9/16 (USB:28): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 20 seconds /var/log/esp/midres/da23dec1415h625ml20s.tif: TIFF Upload Complete @15:31:21.36 Gate.power :camera,:OFF @15:31:21.39 DAfans.email "18.203 sec auto-exposure",Subject: "da23dec1415h625ml images!" @15:31:21.94 Elbow.seek 5 @15:31:22.16 Forearm.seek 5 + 450 counts @15:31:22.24 SE.reconfigure :gently @15:31:22.31 SE.seek 29.53 Pucks below Top Plate @15:31:23.01 Elbow.reconfigure :limp @15:31:23.63 Forearm.reconfigure :loose @15:31:23.68 Forearm.seek 5 @15:31:23.99 Elbow.hold @15:31:24.08 Elbow.reconfigure :tight @15:31:24.13 SE.reconfigure :default @15:31:24.18 SE.seek 29.72 Pucks below Top Plate @15:31:24.62 Forearm.force -20 @15:31:24.67 Forearm.seek 5 + 254 counts @15:31:24.95 Forearm.reconfigure :tight @15:31:25.12 Forearm.seek 5 + 450 counts @15:31:25.61 SE.seek :down @15:31:26.36 SE.stop @15:31:26.91 Forearm.reconfigure :loose @15:31:27.01 Forearm.seek :retract @15:31:28.18 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @15:31:28.58 Elbow.seek FlushPuck::Garage @15:31:30.56 Elbow.reconfigure :loose @15:31:30.76 Forearm.seek :garage @15:31:31.83 Hand.close @15:31:32.83 Forearm.seek :retract @15:31:33.87 Elbow.reconfigure :tight @15:31:34.03 Elbow.seek PC @15:31:35.67 Elbow.reconfigure :loose @15:31:35.88 Forearm.reconfigure :tight @15:31:36.05 Forearm.seek PC @15:31:37.74 PC.seek :closed @15:31:42.56 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.64 @15:31:42.70 Hand.open @15:31:43.51 Forearm.reconfigure :loose @15:31:43.59 Forearm.seek :retract @15:31:44.65 PSR.select :air @15:31:44.69 PSV.dial PSR @15:31:45.21 Pulling 0.5ml of PSR.flush @15:31:45.23 PSR.select :flush @15:31:45.41 PS.seek 0.5ml @15:31:51.43 delay 5 seconds @15:31:54.44 upsync @15:31:56.47 PSR.select :air @15:31:56.59 PS.seek 2.5ml @15:32:17.90 delay 1 second @15:32:18.92 PRV.dial :waste2,avoiding: :ab2 @15:32:20.79 PTV.dial :PRVmixing @15:32:22.12 PSV.dial :mixloop @15:32:23.18 PS.seek :empty @15:32:49.52 PSR.select :air @15:32:49.54 PSV.dial PSR @15:32:50.59 Pulling 0.5ml of PSR.flush @15:32:50.60 PSR.select :flush @15:32:50.71 PS.seek 0.5ml @15:32:56.71 delay 5 seconds @15:33:01.73 PSR.select :air @15:33:01.85 PS.seek 2.5ml @15:33:23.15 delay 1 second @15:33:24.17 PBV.dial :waste1 @15:33:24.54 PTV.dial :mixing @15:33:25.31 PSV.dial :mixloop @15:33:26.36 PS.seek :empty @15:33:52.77 PSV.dial PSR @15:33:53.82 PSR.select :flush @15:33:53.94 PS.seek 1ml @15:34:05.08 PSR.select :air @15:34:05.20 PS.seek 2.5ml @15:34:21.37 PSV.dial PTV @15:34:22.13 PTV.dial :puck @15:34:22.87 PBV.dial :waste1 @15:34:23.05 PS.seek 0ml @15:34:49.40 PSV.dial PSR @15:34:50.15 PSR.select :flush @15:34:50.26 PS.seek 0.5ml @15:34:56.15 PSR.select :air @15:34:56.26 PS.seek 2.5ml @15:35:17.55 PSV.dial PTV @15:35:18.43 PBV.dial PSV @15:35:19.45 PS.seek 0ml @15:35:46.02 PSV.dial PBV @15:35:46.48 PS.seek 2.5ml @15:36:12.89 PSV.dial :waste1 @15:36:13.65 PS.seek 0ml @15:36:40.05 PSV.dial PSR @15:36:40.52 PSR.select :air @15:36:40.55 PS.seek 2.5ml @15:37:06.96 PSV.dial PTV @15:37:07.82 PBV.dial PSV @15:37:08.01 PS.seek 0ml @15:37:34.58 PSV.dial PBV @15:37:35.04 PS.seek 2.5ml @15:38:01.42 PSV.dial :waste1 @15:38:02.17 PS.seek 0ml @15:38:28.52 PSV.dial PSR @15:38:28.99 PSR.select :flush @15:38:29.11 PS.seek 1ml @15:38:40.14 PSR.select :air @15:38:40.26 PS.seek 2.5ml @15:38:56.45 PSV.dial PTV @15:38:57.19 PTV.dial :puck @15:38:57.27 PBV.dial :waste1 @15:38:57.82 PS.seek 0ml @15:39:24.23 PSV.dial PSR @15:39:24.99 PSR.select :flush @15:39:25.10 PS.seek 0.5ml @15:39:31.08 PSR.select :air @15:39:31.20 PS.seek 2.5ml @15:39:52.48 PSV.dial PTV @15:39:53.25 PBV.dial PSV @15:39:53.82 PS.seek 0ml @15:40:20.23 PSV.dial PBV @15:40:20.71 PS.seek 2.5ml @15:40:47.08 PSV.dial :waste1 @15:40:47.82 PS.seek 0ml @15:41:14.20 PSV.dial PSR @15:41:14.67 PSR.select :air @15:41:14.70 PS.seek 2.5ml @15:41:41.11 PSV.dial PTV @15:41:41.84 PBV.dial PSV @15:41:42.04 PS.seek 0ml @15:42:08.42 PSV.dial PBV @15:42:08.96 PS.seek 2.5ml @15:42:35.33 PSV.dial :waste1 @15:42:36.07 PS.seek 0ml @15:43:02.42 Garaging FlushPuck in Processing Clamp @15:43:02.49 Elbow.reconfigure :tight @15:43:02.54 Elbow.seek PC @15:43:02.67 Elbow.reconfigure :loose @15:43:02.77 Forearm.seek PC @15:43:03.78 Hand.close @15:43:04.35 PC.seek :open @15:43:09.06 Forearm.seek :retract @15:43:10.08 Elbow.reconfigure :tight @15:43:10.13 Elbow.seek :garage @15:43:11.43 Elbow.reconfigure :loose @15:43:11.53 Forearm.reconfigure :tight @15:43:11.58 Forearm.seek :garage @15:43:12.65 Hand.open @15:43:13.28 Forearm.reconfigure :loose @15:43:13.33 Forearm.seek :retract @16:07:30.15 Session Begun @16:07:36.10 -> showlog -100 @16:07:40.71 -> Flush FlushPuck is in FlushPuck::Garage @16:07:42.93 -> CC Collection Clamp contains wcr puck from tube 6 @16:07:45.23 -> PC Processing Clamp open @16:07:50.61 -> Puck.count @16:07:50.69 SE.seek :down @16:07:50.78 SE.stop @16:07:50.89 SC.reconfigure :tight @16:07:50.97 SC.seek 1 @16:08:04.01 SC.configure :limp @16:08:04.24 Elbow.reconfigure :tight @16:08:04.31 Elbow.seek 1 @16:08:06.18 Forearm.reconfigure :tight @16:08:06.25 Forearm.seek 1 + 450 counts @16:08:07.30 SE.reconfigure :place @16:08:07.39 SE.seek :beamBreak @16:08:08.53 SE.configure :default @16:08:08.66 SE.seek :down @16:08:09.17 SE.stop @16:08:09.84 Forearm.reconfigure :loose @16:08:09.91 Forearm.seek :retract @16:08:10.88 SC.reconfigure :tight @16:08:10.95 SC.seek 2 @16:08:14.53 SC.configure :limp @16:08:14.71 Elbow.seek 2 @16:08:15.11 Forearm.reconfigure :tight @16:08:15.19 Forearm.seek 2 + 450 counts @16:08:16.20 SE.reconfigure :place @16:08:16.28 SE.seek :beamBreak @16:08:17.46 SE.configure :default @16:08:17.59 SE.seek :down @16:08:18.05 SE.stop @16:08:18.74 Forearm.reconfigure :loose @16:08:18.81 Forearm.seek :retract @16:08:19.79 SC.reconfigure :tight @16:08:19.86 SC.seek 3 @16:08:23.40 SC.configure :limp @16:08:23.58 Elbow.seek 3 @16:08:23.91 Forearm.reconfigure :tight @16:08:23.99 Forearm.seek 3 + 450 counts @16:08:25.02 SE.reconfigure :place @16:08:25.10 SE.seek :beamBreak @16:08:26.25 SE.configure :default @16:08:26.38 SE.seek :down @16:08:26.82 SE.stop @16:08:27.51 Forearm.reconfigure :loose @16:08:27.58 Forearm.seek :retract @16:08:28.53 SC.reconfigure :tight @16:08:28.60 SC.seek 4 @16:08:32.18 SC.configure :limp @16:08:32.35 Elbow.seek 4 @16:08:32.82 Forearm.reconfigure :tight @16:08:32.89 Forearm.seek 4 + 450 counts @16:08:33.93 SE.reconfigure :place @16:08:34.01 SE.seek :beamBreak @16:08:35.60 SE.configure :default @16:08:35.74 SE.seek :down @16:08:36.19 SE.stop @16:08:36.87 Forearm.reconfigure :loose @16:08:36.94 Forearm.seek :retract @16:08:37.91 SC.reconfigure :tight @16:08:37.98 SC.seek 5 @16:08:41.54 SC.configure :limp @16:08:41.72 Elbow.seek 5 @16:08:42.07 Forearm.reconfigure :tight @16:08:42.15 Forearm.seek 5 + 450 counts @16:08:43.22 SE.reconfigure :place @16:08:43.34 SE.seek :beamBreak @16:08:44.62 SE.configure :default @16:08:44.75 SE.seek :down @16:08:45.37 SE.stop @16:08:45.88 Forearm.reconfigure :loose @16:08:45.95 Forearm.seek :retract @16:08:46.92 SC.reconfigure :tight @16:08:47.00 SC.seek 6 @16:08:50.56 SC.configure :limp @16:08:50.73 Elbow.seek 6 @16:08:50.93 Forearm.reconfigure :tight @16:08:51.01 Forearm.seek 6 + 450 counts @16:08:52.06 SE.reconfigure :place @16:08:52.14 SE.seek :beamBreak @16:08:55.37 SE.configure :default @16:08:55.50 SE.seek :down @16:08:56.63 Forearm.reconfigure :loose @16:08:56.70 Forearm.seek :retract @16:08:58.16 SE.stop @16:08:58.22 SC.reconfigure :tight @16:08:58.29 SC.seek 7 @16:09:01.95 SC.configure :limp @16:09:02.12 Elbow.seek 7 @16:09:03.18 Forearm.reconfigure :tight @16:09:03.25 Forearm.seek 7 + 450 counts @16:09:04.31 SE.reconfigure :place @16:09:04.39 SE.seek :beamBreak @16:09:05.70 SE.configure :default @16:09:05.83 SE.seek :down @16:09:06.44 SE.stop @16:09:06.97 Forearm.reconfigure :loose @16:09:07.04 Forearm.seek :retract {1 => 33, 2 => 33, 3 => 33, 4 => 33, 5 => 31, 6 => 1, 7 => 31} @16:10:17.54 -> CC.unloadPuck 5, :WCR @16:10:17.63 Puck::Warning in dave -- Unloading Puck.from 6, :wcr to Storage Carousel @16:10:17.65 Storing WCR puck in Collection Clamp to tube 5 @16:10:17.85 SC.reconfigure :tight @16:10:17.93 SC.seek 5 @16:10:24.69 SC.configure :limp @16:10:24.87 Elbow.seek 5 @16:10:25.41 Forearm.reconfigure :tight @16:10:25.48 Forearm.seek 5 + 450 counts @16:10:26.60 SE.reconfigure :pick @16:10:26.68 SE.seek :beamBreak @16:10:27.77 SE.configure :default @16:10:27.95 Elbow.reconfigure :limp @16:10:28.03 Forearm.reconfigure :loose @16:10:28.10 Forearm.seek 5 @16:10:28.40 Elbow.hold @16:10:28.49 Elbow.reconfigure :tight @16:10:28.61 SE.seek 30.13 Pucks below Top Plate @16:10:29.07 Forearm.force -20 @16:10:29.13 Forearm.seek 5 + 287 counts @16:10:29.43 Forearm.reconfigure :tight @16:10:29.50 Forearm.seek 5 + 450 counts @16:10:29.85 SE.seek 31.13 Pucks below Top Plate @16:10:30.57 Forearm.reconfigure :loose @16:10:30.64 Forearm.seek :retract @16:10:31.59 Elbow.seek CC @16:10:33.30 Elbow.reconfigure :loose @16:10:33.42 Forearm.seek CC @16:10:34.38 Hand.close @16:10:35.08 CC.seek :open @16:10:40.29 Forearm.seek :retract @16:10:41.30 Elbow.reconfigure :tight @16:10:41.37 Elbow.seek 5 @16:10:43.21 Elbow.reconfigure :limp @16:10:43.28 Forearm.seek 5 @16:10:44.34 Elbow.hold @16:10:44.42 Elbow.reconfigure :tight @16:10:44.49 Hand.open @16:10:45.20 Forearm.force -20 @16:10:45.26 Forearm.seek 5 + 316 counts @16:10:45.57 Forearm.reconfigure :tight @16:10:45.64 Forearm.seek 5 + 450 counts @16:10:45.91 SE.seek :down @16:10:46.52 SE.stop @16:10:47.04 Forearm.reconfigure :loose @16:10:47.11 Forearm.seek :retract @16:11:17.53 -> showlog -600 @16:12:33.47 -> showlog -1000 @16:14:33.38 -> Puck.count @16:14:33.46 SE.seek :down @16:14:33.55 SE.stop @16:14:33.66 SC.reconfigure :tight @16:14:33.73 SC.seek 1 @16:14:46.72 SC.configure :limp @16:14:46.90 Elbow.seek 1 @16:14:47.17 Forearm.reconfigure :tight @16:14:47.25 Forearm.seek 1 + 450 counts @16:14:48.39 SE.reconfigure :place @16:14:48.47 SE.seek :beamBreak @16:14:49.58 SE.configure :default @16:14:50.16 SE.seek :down @16:14:50.66 SE.stop @16:14:51.29 Forearm.reconfigure :loose @16:14:51.36 Forearm.seek :retract @16:14:52.34 SC.reconfigure :tight @16:14:52.41 SC.seek 2 @16:14:55.99 SC.configure :limp @16:14:56.16 Elbow.seek 2 @16:14:56.37 Forearm.reconfigure :tight @16:14:56.45 Forearm.seek 2 + 450 counts @16:14:57.48 SE.reconfigure :place @16:14:57.56 SE.seek :beamBreak @16:14:58.73 SE.configure :default @16:14:58.86 SE.seek :down @16:14:59.31 SE.stop @16:14:59.98 Forearm.reconfigure :loose @16:15:00.06 Forearm.seek :retract @16:15:01.03 SC.reconfigure :tight @16:15:01.10 SC.seek 3 @16:15:04.63 SC.configure :limp @16:15:04.81 Elbow.seek 3 @16:15:05.33 Forearm.reconfigure :tight @16:15:05.40 Forearm.seek 3 + 450 counts @16:15:06.44 SE.reconfigure :place @16:15:06.52 SE.seek :beamBreak @16:15:07.65 SE.configure :default @16:15:07.78 SE.seek :down @16:15:08.24 SE.stop @16:15:08.90 Forearm.reconfigure :loose @16:15:08.98 Forearm.seek :retract @16:15:09.95 SC.reconfigure :tight @16:15:10.02 SC.seek 4 @16:15:13.60 SC.configure :limp @16:15:13.77 Elbow.seek 4 @16:15:14.25 Forearm.reconfigure :tight @16:15:14.32 Forearm.seek 4 + 450 counts @16:15:15.84 SE.reconfigure :place @16:15:15.93 SE.seek :beamBreak @16:15:17.07 SE.configure :default @16:15:17.21 SE.seek :down @16:15:17.66 SE.stop @16:15:18.33 Forearm.reconfigure :loose @16:15:18.41 Forearm.seek :retract @16:15:19.39 SC.reconfigure :tight @16:15:19.46 SC.seek 5 @16:15:23.02 SC.configure :limp @16:15:23.20 Elbow.seek 5 @16:15:23.47 Forearm.reconfigure :tight @16:15:23.55 Forearm.seek 5 + 450 counts @16:15:24.60 SE.reconfigure :place @16:15:24.68 SE.seek :beamBreak @16:15:26.02 SE.configure :default @16:15:26.15 SE.seek :down @16:15:26.67 SE.stop @16:15:27.28 Forearm.reconfigure :loose @16:15:27.35 Forearm.seek :retract @16:15:28.30 SC.reconfigure :tight @16:15:28.37 SC.seek 6 @16:15:31.91 SC.configure :limp @16:15:32.08 Elbow.seek 6 @16:15:32.30 Forearm.reconfigure :tight @16:15:32.37 Forearm.seek 6 + 450 counts @16:15:33.46 SE.reconfigure :place @16:15:33.55 SE.seek :beamBreak @16:15:36.83 SE.configure :default @16:15:36.96 SE.seek :down @16:15:38.10 Forearm.reconfigure :loose @16:15:38.17 Forearm.seek :retract @16:15:39.59 SE.stop @16:15:39.65 SC.reconfigure :tight @16:15:39.73 SC.seek 7 @16:15:43.24 SC.configure :limp @16:15:43.81 Elbow.seek 7 @16:15:44.24 Forearm.reconfigure :tight @16:15:44.32 Forearm.seek 7 + 450 counts @16:15:45.49 SE.reconfigure :place @16:15:45.58 SE.seek :beamBreak @16:15:46.88 SE.configure :default @16:15:47.01 SE.seek :down @16:15:47.66 SE.stop @16:15:48.15 Forearm.reconfigure :loose @16:15:48.23 Forearm.seek :retract {1 => 33, 2 => 33, 3 => 33, 4 => 33, 5 => 32, 6 => 1, 7 => 31} @16:16:51.57 -> abort! 'mission complete' @16:16:51.59 CmdServer::Client::Error in dave -- Aborting Mission: mission complete @16:16:51.63 BadNews.email "mission complete",Subject: "DA_WCR_121423 ABORTED!" # @16:16:56.26 upsync @16:16:56.36 Session Ended + ESPwhich DA_WCR_121523.rb + fn=DA_WCR_121523.rb + IFS=: + set /home/esp/esp2/mission . + fullPath=/home/esp/esp2/mission/DA_WCR_121523.rb + [ -f /home/esp/esp2/mission/DA_WCR_121523.rb ] + realpath /home/esp/esp2/mission/DA_WCR_121523.rb + return 0 + scriptPath=/home/esp/esp2local/mission/DA_WCR_121523.rb + exec ruby /home/esp/esp2/lib/esp.rb /home/esp/esp2local/mission/DA_WCR_121523.rb @13:35:40.19UTC15-Dec-23
ESP2 v3.42 I2C Gateway v3.20 for core bus @13:35:42.89 Configuration for short puck jake 1/11/21 by brent@mbari.org /home/esp/esp2/type/shallow/jake/configure.rb @13:35:46.61 Polling Can every 10:00 @13:35:46.83 Cleanly exited at 01:27:51UTC15-Dec-23 @13:35:46.88 Loading /home/esp/esp2local/mission/DA_WCR_121523.rb mission @13:35:46.94 Preparing to sleep until + 00:00:05 @13:35:46.96 GoodNews.email "Starting DA_WCR_121523 w/pucks in tube 6\n{1 => 33,\n 2 => 33,\n 3 => 33,\n 4 => 33,\n 5 => 32,\n 6 => 1,\n 7 => 31}\nCTD data unavailable\nCan@13:35:46, 31% flow, 16.0C, 34% humidity, 15.1psia, 13.754V, 0.266A, 227.667Ah, 3.66W",Subject: "DA_WCR_121523 begun" @13:35:47.02
Asleep til + 00:00:05 @13:35:47.03 Continuing at 13:35 @13:35:47.28 Gate.power :core,:OFF @13:35:47.30 Gate.power :raw,:OFF @13:35:47.31 Shutdown @13:35:50.48 GoodNews.email "Then, in tube 6: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_121523.rb:14\n{1 => 33,\n 2 => 33,\n 3 => 33,\n 4 => 33,\n 5 => 32,\n 6 => 1,\n 7 => 31}\nCTD data unavailable\nCan@13:35:47, 31% flow, 16.0C, 34% humidity, 15.1psia, 13.754V, 0.266A, 227.667Ah, 3.66W",Subject: "Asleep until 13:35" @13:35:51.87 upsync @13:35:51.94
Powering core bus @13:35:55.28 upsync @13:35:57.17
Gate.power :raw,:ON @13:35:58.13 CS.configure :default @13:35:58.18 PS.configure :default @13:35:58.23 SS.configure :default @13:35:58.27 CC.configure :default @13:35:58.32 PC.configure :default @13:35:58.39 Forearm.configure :loose @13:35:58.44 Elbow.configure :loose @13:35:58.51 SC.configure :loose @13:35:58.55 SE.configure :default @13:35:58.60 CH.configure :default @13:35:58.64 PH.configure :default @13:35:58.68 All dwarf controllers running firmware version 3.85 @13:35:58.78 Valve.reset @13:35:59.18 PSV.dial :air @13:35:59.20 Intake.select :close @13:35:59.23 Exhaust.select :close @13:35:59.26 CTV.dial :air @13:35:59.39
Forearm.home.seek :retract @13:36:00.13 Hand.close @13:36:00.16
Elbow.reconfigure :loose @13:36:00.25 Elbow.jog 1500 @13:36:00.32 PS.home.seek 0ml @13:36:00.58 CSV.dial :waste1 @13:36:00.66
Elbow.reconfigure :loose @13:36:00.71 Elbow.home.seek :clear @13:36:01.31 SSV.dial :spit,avoiding: :exhaust @13:36:02.18 CS.reconfigure :fast @13:36:02.23 SS.home.seek 0ml @13:36:02.32 CS.home.seek 0ml @13:36:04.05
SE.home.seek :down @13:36:04.66 SC.reconfigure :tight @13:36:04.71 SC.home.seek :initial @13:36:08.65 PS.stop @13:36:33.03
SC.configure :limp @13:36:33.08 Forearm.seek :retract @13:36:33.15 Elbow.seek :hammer @13:36:34.19 Forearm.reconfigure :tight @13:36:34.24 Forearm.seek retracted @13:36:34.91 PC.home.seek :open @13:36:34.94
CC.home.seek :open @13:36:38.33 Notice.email "At 13:36:38 began in tube 6: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_121523.rb:14\n{1 => 33,\n 2 => 33,\n 3 => 33,\n 4 => 33,\n 5 => 32,\n 6 => 1,\n 7 => 31}\nCTD data unavailable\nCan@13:36:38, 31% flow, 16.1C, 34% humidity, 15.1psia, 13.681V, 0.290A, 227.673Ah, 3.97W",Subject: "Started DA_WCR_121523 phase initialPurge; da [1000, 1000] #da and wsr are run simultanelously" @13:36:38.36
Phase [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] @13:36:38.37 Starting initialPurge @13:36:38.38 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @13:36:38.45 Hand.open @13:36:39.15 Elbow.reconfigure :tight @13:36:39.19 Elbow.seek FlushPuck::Garage @13:36:41.09 Elbow.reconfigure :loose @13:36:41.19 Forearm.reconfigure :loose @13:36:41.24 Forearm.seek :garage @13:36:42.29 Hand.close @13:36:42.74 upsync @13:36:43.01
Forearm.seek :retract @13:36:43.98 Elbow.reconfigure :tight @13:36:44.03 Elbow.seek CC @13:36:47.03 Elbow.reconfigure :loose @13:36:47.14 Forearm.reconfigure :tight @13:36:47.19 Forearm.seek CC @13:36:48.47 CC.seek :closed @13:36:53.05 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.43 @13:36:53.12 Hand.open @13:36:53.82 Forearm.reconfigure :loose @13:36:53.86 Forearm.seek :retract @13:36:54.84 Flushing the Collection Valve::Manifold @13:36:54.97 CSV.dial CSR @13:36:56.27 Pulling 5ml of CSR.flush @13:36:56.28 CSR.select :flush @13:36:56.39 CS.reconfigure :default @13:36:56.85 CS.seek 5ml @13:37:10.52 delay 5 seconds @13:37:15.54 CSR.select :air @13:37:15.70 CS.reconfigure :fast @13:37:15.75 CS.seek 7.501ml @13:37:22.58 CSV.dial :waste @13:37:23.88 CS.reconfigure :default @13:37:23.93 CS.seek 0ml @13:37:44.02 Purging external Intake @13:37:44.14 CSV.dial :flush @13:37:44.60 CS.reconfigure :fast @13:37:44.65 CS.seek 10ml @13:38:09.20 delay 5 seconds @13:38:14.22 CSV.dial CBV @13:38:14.25
CBV.dial CSV @13:38:15.06 CTV.dial :intake @13:38:15.83 Intake.select :open @13:38:15.94 CS.seek 0ml @13:38:39.67 Intake.select :close @13:38:39.77 CTV.dial :air @13:38:40.55 Flushing upper manifold @13:38:40.67 CSV.dial :flush @13:38:41.41 CS.seek 5ml @13:38:54.30 delay 5 seconds @13:38:59.31 CSV.dial :air @13:38:59.93 CS.seek 7.001ml @13:39:05.58 CSV.dial CBV @13:39:05.60
CBV.dial CSV @13:39:06.62 CTV.dial :air @13:39:06.64 CS.seek 0ml @13:39:23.26 delay 7 seconds @13:39:30.29 CS.seek 3ml @13:39:38.32 CSV.dial :waste @13:39:38.79 CS.seek 0ml @13:39:46.62 CSV.dial :flush @13:39:47.07 CS.seek 3ml @13:39:55.14 delay 5 seconds @13:40:00.16 CSV.dial :air @13:40:00.77 CS.seek 5ml @13:40:06.48 CSV.dial :air @13:40:06.57 CS.seek 0ml @13:40:18.95 delay 7 seconds @13:40:25.98 CS.seek 3ml @13:40:33.95 CSV.dial :waste @13:40:34.68 CS.seek 0ml @13:40:42.42 Garaging FlushPuck in Collection Clamp @13:40:42.49 Elbow.reconfigure :tight @13:40:42.54 Elbow.seek CC @13:40:42.85 Elbow.reconfigure :loose @13:40:42.96 Forearm.seek CC @13:40:44.03 Hand.close @13:40:44.63 CC.seek :open @13:40:49.66 Forearm.seek :retract @13:40:50.63 Elbow.reconfigure :tight @13:40:50.68 Elbow.seek :garage @13:40:53.53 Elbow.reconfigure :loose @13:40:53.63 Forearm.reconfigure :tight @13:40:53.67 Forearm.seek :garage @13:40:54.69 Hand.open @13:40:55.31 Forearm.reconfigure :loose @13:40:55.36 Forearm.seek :retract @13:40:56.35 da sampling at most 1000ml, wcr at most 1000ml @13:40:56.39 Loading sh1 puck from tube 6 into Collection Clamp @13:40:56.41 DAfans.email "Started da in tube 6 at 13:40:56UTC15-Dec-23\n{1 => 33,\n 2 => 33,\n 3 => 33,\n 4 => 33,\n 5 => 32,\n 6 => 1,\n 7 => 31}\nCTD data unavailable\nCan@13:36:38, 31% flow, 16.1C, 34% humidity, 15.1psia, 13.681V, 0.290A, 227.673Ah, 3.97W",Subject: "da sampling at most 1000ml, wcr at most 1000ml" @13:40:56.68
SC.reconfigure :tight @13:40:56.74 SC.seek 6 @13:41:00.79 upsync @13:41:03.50
SC.configure :limp @13:41:03.73 Elbow.reconfigure :tight @13:41:03.78 Elbow.seek 6 @13:41:05.70 Forearm.reconfigure :tight @13:41:05.75 Forearm.seek 6 + 450 counts @13:41:06.78 SE.reconfigure :pick @13:41:06.84 SE.seek :beamBreak @13:41:09.94 SE.configure :default @13:41:10.10 Elbow.seek 6 @13:41:10.29 Forearm.seek 6 + 300 counts @13:41:10.50 Forearm.reconfigure :loose @13:41:10.55 Hand.close @13:41:11.15 SE.seek :down @13:41:11.36
Forearm.seek :retract @13:41:12.75 Elbow.seek CC @13:41:13.95 SE.stop @13:41:14.36
Elbow.reconfigure :loose @13:41:14.46 Forearm.reconfigure :tight @13:41:14.51 Forearm.seek CC @13:41:15.72 CC.seek :closed @13:41:20.24 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.34 @13:41:20.25 Hand.open @13:41:20.93 Forearm.reconfigure :loose @13:41:20.98 Forearm.seek :retract @13:41:21.92 collectSample 1000ml w/bubble point of 24psi @13:41:21.94 Priming 7ml @13:41:21.95 Intake.select :close @13:41:21.96 Exhaust.select :close @13:41:21.97 SS.hold @13:41:22.18 Calibrating pressure and temperature sensors @13:41:22.21 CTV.dial :air @13:41:22.22 SSV.dial :spit,avoiding: :exhaust @13:41:22.42 SS.reconfigure :fast @13:41:22.47 SS.seek 5ml @13:41:25.35 SSV.dial CBV @13:41:25.39
CBV.dial SSV @13:41:26.71 delay 3.8 seconds @13:41:30.64 Sensor limits: Intake max 99.5psia; Outlet max 92.7psia @13:41:30.65 SSV.dial :spit @13:41:31.97 SS.seek 0ml @13:41:34.59 SSV.dial CBV @13:41:35.92 CTV.dial :intake @13:41:36.70 SSV.dial CBV @13:41:36.72
CBV.dial SSV @13:41:36.95 Allowing 40s for 7.0ml of remaining 7.0ml @13:41:36.96 Intake.select :open @13:41:39.12 Intake @14.6psia, vacuum from 9.1 to 12.1psi @13:41:39.14 SS.reconfigure :inhaling 2.20ml/s @13:41:39.18 SS.seek 7ml @13:41:45.72 Stroke completed -- waiting for pressure to equalize ... @13:41:45.73 SS.reconfigure :exhale @13:41:45.78 SS.hold @13:41:54.52 Intake.select :close @13:41:54.67 SSV.dial :exhaust @13:41:55.43 Exhaust.select :open @13:41:55.54 SS.seek 0ml @13:41:59.55 Exhaust.select :close @13:41:59.66 Sampling 1000.0ml @13:41:59.68 Intake.select :close @13:41:59.69 Exhaust.select :close @13:41:59.70 SS.reconfigure :default @13:41:59.74 SS.hold @13:41:59.95 CTV.dial :intake @13:42:00.12 SSV.dial CBV @13:42:00.14
CBV.dial SSV @13:42:00.93 Allowing 130s for 25.0ml of remaining 1000.0ml @13:42:00.94 Intake.select :open @13:42:03.11 Intake @15.3psia, vacuum from 9.8 to 12.8psi @13:42:03.12 SS.reconfigure :inhaling 2.20ml/s @13:42:03.17 SS.seek 25ml @13:42:10.80 Vacuum too high -- waiting up to 40 seconds @13:42:10.81 SS.reconfigure :exhale @13:42:10.86 SS.hold @13:42:16.44 Intake @18.2psia, vacuum from 10.0 to 13.0psi @13:42:16.53 SS.reconfigure :inhaling 1.76ml/s @13:42:16.58 SS.seek 25ml @13:42:18.95 Vacuum too high -- waiting up to 40 seconds @13:42:18.97 SS.reconfigure :exhale @13:42:19.02 SS.hold @13:42:21.32 Intake @18.7psia, vacuum from 10.0 to 13.0psi @13:42:21.34 SS.reconfigure :inhaling 1.41ml/s @13:42:21.39 SS.seek 25ml @13:42:23.64 Vacuum too high -- waiting up to 40 seconds @13:42:23.65 SS.reconfigure :exhale @13:42:23.71 SS.hold @13:42:25.46 SS.reconfigure :inhaling 1.13ml/s @13:42:25.51 SS.seek 25ml @13:42:28.30 Vacuum too high -- waiting up to 40 seconds @13:42:28.31 SS.reconfigure :exhale @13:42:28.36 SS.hold @13:42:29.80 Intake @18.8psia, vacuum from 10.0 to 13.0psi @13:42:29.82 SS.reconfigure :inhaling 0.91ml/s @13:42:29.87 SS.seek 25ml @13:42:33.17 Vacuum too high -- waiting up to 40 seconds @13:42:33.18 SS.reconfigure :exhale @13:42:33.23 SS.hold @13:42:34.57 Intake @18.8psia, vacuum from 10.0 to 13.0psi @13:42:34.58 SS.reconfigure :inhaling 0.73ml/s @13:42:34.63 SS.seek 25ml @13:42:40.91 Stroke completed -- waiting for pressure to equalize ... @13:42:40.92 SS.reconfigure :exhale @13:42:40.97 SS.hold @13:43:05.73 Intake.select :close @13:43:05.89 SSV.dial :exhaust @13:43:06.65 Exhaust.select :open @13:43:06.76 SS.seek 0ml @13:43:15.61 Exhaust.select :close @13:43:15.73 SSV.dial CBV @13:43:15.75
CBV.dial SSV @13:43:16.54 Allowing 130s for 25.0ml of remaining 975.0ml @13:43:16.55 Intake.select :open @13:43:18.72 Intake @15.9psia, vacuum from 10.0 to 13.0psi @13:43:18.73 SS.reconfigure :inhaling 0.73ml/s @13:43:18.78 SS.seek 25ml @13:43:55.24 Stroke completed -- waiting for pressure to equalize ... @13:43:55.25 SS.reconfigure :exhale @13:43:55.29 SS.hold @13:44:24.08 Intake.select :close @13:44:24.23 SSV.dial :exhaust @13:44:24.99 Exhaust.select :open @13:44:25.10 SS.seek 0ml @13:44:33.93 Exhaust.select :close @13:44:34.03 SSV.dial CBV @13:44:34.06
CBV.dial SSV @13:44:34.83 Allowing 130s for 25.0ml of remaining 950.0ml @13:44:34.84 Intake.select :open @13:44:37.01 Intake @16.0psia, vacuum from 10.0 to 13.0psi @13:44:37.02 SS.reconfigure :inhaling 0.73ml/s @13:44:37.07 SS.seek 25ml @13:45:13.50 Stroke completed -- waiting for pressure to equalize ... @13:45:13.51 SS.reconfigure :exhale @13:45:13.56 SS.hold @13:45:40.34 Intake.select :close @13:45:40.49 SSV.dial :exhaust @13:45:41.26 Exhaust.select :open @13:45:41.38 SS.seek 0ml @13:45:50.38 Exhaust.select :close @13:45:50.49 SSV.dial CBV @13:45:50.51
CBV.dial SSV @13:45:51.30 Allowing 130s for 25.0ml of remaining 925.0ml @13:45:51.31 Intake.select :open @13:45:53.48 Intake @16.0psia, vacuum from 10.0 to 13.0psi @13:45:53.49 SS.reconfigure :inhaling 0.73ml/s @13:45:53.54 SS.seek 25ml @13:46:29.52 Stroke completed -- waiting for pressure to equalize ... @13:46:29.53 SS.reconfigure :exhale @13:46:29.57 SS.hold @13:46:54.37 Intake.select :close @13:46:54.53 SSV.dial :exhaust @13:46:55.28 Exhaust.select :open @13:46:55.40 SS.seek 0ml @13:47:04.11 Exhaust.select :close @13:47:04.22 SSV.dial CBV @13:47:04.24
CBV.dial SSV @13:47:05.03 Allowing 130s for 25.0ml of remaining 900.0ml @13:47:05.04 Intake.select :open @13:47:07.21 Intake @16.1psia, vacuum from 10.0 to 13.0psi @13:47:07.22 SS.reconfigure :inhaling 0.73ml/s @13:47:07.27 SS.seek 25ml @13:47:43.75 Stroke completed -- waiting for pressure to equalize ... @13:47:43.76 SS.reconfigure :exhale @13:47:43.81 SS.hold @13:48:04.51 Intake.select :close @13:48:04.66 SSV.dial :exhaust @13:48:05.43 Exhaust.select :open @13:48:05.54 SS.seek 0ml @13:48:14.44 Exhaust.select :close @13:48:14.54 SSV.dial CBV @13:48:14.56
CBV.dial SSV @13:48:15.34 Allowing 130s for 25.0ml of remaining 875.0ml @13:48:15.36 Intake.select :open @13:48:17.52 Intake @16.1psia, vacuum from 10.0 to 13.0psi @13:48:17.54 SS.reconfigure :inhaling 0.73ml/s @13:48:17.58 SS.seek 25ml @13:48:53.39 Stroke completed -- waiting for pressure to equalize ... @13:48:53.40 SS.reconfigure :exhale @13:48:53.44 SS.hold @13:49:13.88 Intake.select :close @13:49:14.04 SSV.dial :exhaust @13:49:14.85 Exhaust.select :open @13:49:14.97 SS.seek 0ml @13:49:23.79 Exhaust.select :close @13:49:23.90 SSV.dial CBV @13:49:23.93
CBV.dial SSV @13:49:24.69 Allowing 130s for 25.0ml of remaining 850.0ml @13:49:24.70 Intake.select :open @13:49:26.86 Intake @16.0psia, vacuum from 10.0 to 13.0psi @13:49:26.87 SS.reconfigure :inhaling 0.73ml/s @13:49:26.92 SS.seek 25ml @13:50:02.79 Stroke completed -- waiting for pressure to equalize ... @13:50:02.80 SS.reconfigure :exhale @13:50:02.85 SS.hold @13:50:21.22 Intake.select :close @13:50:21.37 SSV.dial :exhaust @13:50:22.14 Exhaust.select :open @13:50:22.25 SS.seek 0ml @13:50:30.93 Exhaust.select :close @13:50:31.04 SSV.dial CBV @13:50:31.08
CBV.dial SSV @13:50:31.83 Allowing 130s for 25.0ml of remaining 825.0ml @13:50:31.84 Intake.select :open @13:50:34.00 Intake @16.1psia, vacuum from 10.0 to 13.0psi @13:50:34.01 SS.reconfigure :inhaling 0.73ml/s @13:50:34.06 SS.seek 25ml @13:51:10.53 Stroke completed -- waiting for pressure to equalize ... @13:51:10.54 SS.reconfigure :exhale @13:51:10.58 SS.hold @13:51:28.99 Intake.select :close @13:51:29.15 SSV.dial :exhaust @13:51:29.92 Exhaust.select :open @13:51:30.03 SS.seek 0ml @13:51:38.70 Exhaust.select :close @13:51:38.81 SSV.dial CBV @13:51:38.83
CBV.dial SSV @13:51:39.61 Allowing 130s for 25.0ml of remaining 800.0ml @13:51:39.62 Intake.select :open @13:51:41.78 Intake @16.0psia, vacuum from 10.0 to 13.0psi @13:51:41.80 SS.reconfigure :inhaling 0.73ml/s @13:51:41.85 SS.seek 25ml @13:52:18.21 Stroke completed -- waiting for pressure to equalize ... @13:52:18.22 SS.reconfigure :exhale @13:52:18.27 SS.hold @13:52:34.61 Intake.select :close @13:52:35.19 SSV.dial :exhaust @13:52:35.95 Exhaust.select :open @13:52:36.07 SS.seek 0ml @13:52:44.65 Exhaust.select :close @13:52:44.77 SSV.dial CBV @13:52:44.79
CBV.dial SSV @13:52:45.57 Allowing 130s for 25.0ml of remaining 775.0ml @13:52:45.58 Intake.select :open @13:52:47.76 Intake @16.0psia, vacuum from 10.0 to 13.0psi @13:52:47.77 SS.reconfigure :inhaling 0.73ml/s @13:52:47.82 SS.seek 25ml @13:53:24.20 Stroke completed -- waiting for pressure to equalize ... @13:53:24.21 SS.reconfigure :exhale @13:53:24.26 SS.hold @13:53:42.50 Intake.select :close @13:53:42.65 SSV.dial :exhaust @13:53:43.42 Exhaust.select :open @13:53:43.53 SS.seek 0ml @13:53:52.18 Exhaust.select :close @13:53:52.29 SSV.dial CBV @13:53:52.31
CBV.dial SSV @13:53:53.10 Allowing 130s for 25.0ml of remaining 750.0ml @13:53:53.11 Intake.select :open @13:53:55.71 Intake @16.1psia, vacuum from 10.0 to 13.0psi @13:53:55.72 SS.reconfigure :inhaling 0.73ml/s @13:53:55.78 SS.seek 25ml @13:54:32.27 Stroke completed -- waiting for pressure to equalize ... @13:54:32.28 SS.reconfigure :exhale @13:54:32.33 SS.hold @13:54:50.68 Intake.select :close @13:54:50.83 SSV.dial :exhaust @13:54:51.59 Exhaust.select :open @13:54:51.70 SS.seek 0ml @13:55:00.43 Exhaust.select :close @13:55:00.54 SSV.dial CBV @13:55:00.56
CBV.dial SSV @13:55:01.34 Allowing 130s for 25.0ml of remaining 725.0ml @13:55:01.35 Intake.select :open @13:55:03.51 Intake @16.0psia, vacuum from 10.0 to 13.0psi @13:55:03.52 SS.reconfigure :inhaling 0.73ml/s @13:55:03.57 SS.seek 25ml @13:55:39.99 Stroke completed -- waiting for pressure to equalize ... @13:55:40.00 SS.reconfigure :exhale @13:55:40.05 SS.hold @13:56:00.64 Intake.select :close @13:56:00.79 SSV.dial :exhaust @13:56:01.56 Exhaust.select :open @13:56:01.67 SS.seek 0ml @13:56:10.41 Exhaust.select :close @13:56:10.52 SSV.dial CBV @13:56:10.54
CBV.dial SSV @13:56:11.33 Allowing 130s for 25.0ml of remaining 700.0ml @13:56:11.34 Intake.select :open @13:56:13.51 Intake @16.0psia, vacuum from 10.0 to 13.0psi @13:56:13.52 SS.reconfigure :inhaling 0.73ml/s @13:56:13.57 SS.seek 25ml @13:56:49.89 Stroke completed -- waiting for pressure to equalize ... @13:56:49.90 SS.reconfigure :exhale @13:56:49.95 SS.hold @13:57:08.82 Intake.select :close @13:57:08.97 SSV.dial :exhaust @13:57:09.74 Exhaust.select :open @13:57:09.86 SS.seek 0ml @13:57:18.80 Exhaust.select :close @13:57:18.91 SSV.dial CBV @13:57:18.93
CBV.dial SSV @13:57:19.72 Allowing 130s for 25.0ml of remaining 675.0ml @13:57:19.73 Intake.select :open @13:57:21.90 Intake @16.0psia, vacuum from 10.0 to 13.0psi @13:57:21.92 SS.reconfigure :inhaling 0.73ml/s @13:57:21.96 SS.seek 25ml @13:57:57.69 Stroke completed -- waiting for pressure to equalize ... @13:57:57.70 SS.reconfigure :exhale @13:57:57.75 SS.hold @13:58:20.58 Intake.select :close @13:58:20.73 SSV.dial :exhaust @13:58:21.49 Exhaust.select :open @13:58:21.60 SS.seek 0ml @13:58:30.35 Exhaust.select :close @13:58:30.45 SSV.dial CBV @13:58:30.48
CBV.dial SSV @13:58:31.25 Allowing 130s for 25.0ml of remaining 650.0ml @13:58:31.26 Intake.select :open @13:58:33.43 Intake @16.0psia, vacuum from 10.0 to 13.0psi @13:58:33.45 SS.reconfigure :inhaling 0.73ml/s @13:58:33.49 SS.seek 25ml @13:59:09.88 Stroke completed -- waiting for pressure to equalize ... @13:59:09.89 SS.reconfigure :exhale @13:59:09.94 SS.hold @13:59:34.51 Intake.select :close @13:59:34.67 SSV.dial :exhaust @13:59:35.42 Exhaust.select :open @13:59:35.54 SS.seek 0ml @13:59:44.50 Exhaust.select :close @13:59:44.61 SSV.dial CBV @13:59:44.63
CBV.dial SSV @13:59:45.42 Allowing 130s for 25.0ml of remaining 625.0ml @13:59:45.43 Intake.select :open @13:59:47.60 Intake @16.0psia, vacuum from 10.0 to 13.0psi @13:59:47.62 SS.reconfigure :inhaling 0.73ml/s @13:59:47.66 SS.seek 25ml @14:00:15.52 Vacuum too high -- waiting up to 40 seconds @14:00:15.54 SS.reconfigure :exhale @14:00:15.59 SS.hold @14:00:23.51 Intake @15.5psia, vacuum from 10.0 to 13.0psi @14:00:23.60 SS.reconfigure :inhaling 0.59ml/s @14:00:23.65 SS.seek 25ml @14:00:34.02 Stroke completed -- waiting for pressure to equalize ... @14:00:34.03 SS.reconfigure :exhale @14:00:34.07 SS.hold @14:00:52.44 Intake.select :close @14:00:52.60 SSV.dial :exhaust @14:00:53.36 Exhaust.select :open @14:00:53.47 SS.seek 0ml @14:01:02.14 Exhaust.select :close @14:01:02.25 SSV.dial CBV @14:01:02.27
CBV.dial SSV @14:01:03.06 Allowing 130s for 25.0ml of remaining 600.0ml @14:01:03.07 Intake.select :open @14:01:05.24 Intake @16.0psia, vacuum from 10.0 to 13.0psi @14:01:05.25 SS.reconfigure :inhaling 0.59ml/s @14:01:05.30 SS.seek 25ml @14:01:50.09 Stroke completed -- waiting for pressure to equalize ... @14:01:50.10 SS.reconfigure :exhale @14:01:50.15 SS.hold @14:02:12.92 Intake.select :close @14:02:13.07 SSV.dial :exhaust @14:02:13.83 Exhaust.select :open @14:02:13.94 SS.seek 0ml @14:02:22.61 Exhaust.select :close @14:02:22.72 SSV.dial CBV @14:02:22.74
CBV.dial SSV @14:02:23.53 Allowing 130s for 25.0ml of remaining 575.0ml @14:02:23.54 Intake.select :open @14:02:25.71 Intake @16.1psia, vacuum from 10.0 to 13.0psi @14:02:25.72 SS.reconfigure :inhaling 0.59ml/s @14:02:25.77 SS.seek 25ml @14:03:06.80 Vacuum too high -- waiting up to 40 seconds @14:03:06.81 SS.reconfigure :exhale @14:03:06.87 SS.hold @14:03:16.95 Intake @17.3psia, vacuum from 10.0 to 13.0psi @14:03:16.96 SS.reconfigure :inhaling 0.47ml/s @14:03:17.01 SS.seek 25ml @14:03:21.97 Stroke completed -- waiting for pressure to equalize ... @14:03:21.98 SS.reconfigure :exhale @14:03:22.03 SS.hold @14:03:38.36 Intake.select :close @14:03:38.52 SSV.dial :exhaust @14:03:39.28 Exhaust.select :open @14:03:39.40 SS.seek 0ml @14:03:48.12 Exhaust.select :close @14:03:48.23 SSV.dial CBV @14:03:48.26
CBV.dial SSV @14:03:49.03 Allowing 130s for 25.0ml of remaining 550.0ml @14:03:49.04 Intake.select :open @14:03:51.21 Intake @16.0psia, vacuum from 10.0 to 13.0psi @14:03:51.23 SS.reconfigure :inhaling 0.47ml/s @14:03:51.27 SS.seek 25ml @14:04:46.65 Stroke completed -- waiting for pressure to equalize ... @14:04:46.66 SS.reconfigure :exhale @14:04:46.71 SS.hold @14:05:09.26 Intake.select :close @14:05:09.41 SSV.dial :exhaust @14:05:10.17 Exhaust.select :open @14:05:10.29 SS.seek 0ml @14:05:19.06 Exhaust.select :close @14:05:19.17 SSV.dial CBV @14:05:19.19
CBV.dial SSV @14:05:19.95 Allowing 130s for 25.0ml of remaining 525.0ml @14:05:19.96 Intake.select :open @14:05:22.13 Intake @16.1psia, vacuum from 10.0 to 13.0psi @14:05:22.14 SS.reconfigure :inhaling 0.47ml/s @14:05:22.20 SS.seek 25ml @14:06:17.81 Stroke completed -- waiting for pressure to equalize ... @14:06:17.82 SS.reconfigure :exhale @14:06:17.86 SS.hold @14:06:42.78 Intake.select :close @14:06:42.93 SSV.dial :exhaust @14:06:43.68 Exhaust.select :open @14:06:43.80 SS.seek 0ml @14:06:52.57 Exhaust.select :close @14:06:52.68 SSV.dial CBV @14:06:52.72
CBV.dial SSV @14:06:53.46 Allowing 130s for 25.0ml of remaining 500.0ml @14:06:53.47 Intake.select :open @14:06:55.64 Intake @16.1psia, vacuum from 10.0 to 13.0psi @14:06:55.65 SS.reconfigure :inhaling 0.47ml/s @14:06:55.75 SS.seek 25ml @14:07:51.33 Stroke completed -- waiting for pressure to equalize ... @14:07:51.34 SS.reconfigure :exhale @14:07:51.39 SS.hold @14:08:20.01 Intake.select :close @14:08:20.17 SSV.dial :exhaust @14:08:20.93 Exhaust.select :open @14:08:21.05 SS.seek 0ml @14:08:29.88 Exhaust.select :close @14:08:29.99 SSV.dial CBV @14:08:30.02
CBV.dial SSV @14:08:30.79 Allowing 130s for 25.0ml of remaining 475.0ml @14:08:30.80 Intake.select :open @14:08:32.97 Intake @16.1psia, vacuum from 10.0 to 13.0psi @14:08:32.99 SS.reconfigure :inhaling 0.47ml/s @14:08:33.03 SS.seek 25ml @14:09:28.75 Stroke completed -- waiting for pressure to equalize ... @14:09:28.76 SS.reconfigure :exhale @14:09:28.81 SS.hold @14:10:01.54 Intake.select :close @14:10:01.69 SSV.dial :exhaust @14:10:02.44 Exhaust.select :open @14:10:02.56 SS.seek 0ml @14:10:11.35 Exhaust.select :close @14:10:11.46 SSV.dial CBV @14:10:11.48
CBV.dial SSV @14:10:12.29 Allowing 130s for 25.0ml of remaining 450.0ml @14:10:12.30 Intake.select :open @14:10:14.46 Intake @16.3psia, vacuum from 10.0 to 13.0psi @14:10:14.47 SS.reconfigure :inhaling 0.47ml/s @14:10:14.52 SS.seek 25ml @14:11:03.26 Vacuum too high -- waiting up to 40 seconds @14:11:03.28 SS.reconfigure :exhale @14:11:03.33 SS.hold @14:11:16.50 Intake @16.7psia, vacuum from 10.0 to 13.0psi @14:11:16.59 SS.reconfigure :inhaling 0.38ml/s @14:11:16.64 SS.seek 25ml @14:11:25.38 Stroke completed -- waiting for pressure to equalize ... @14:11:25.39 SS.reconfigure :exhale @14:11:25.44 SS.hold @14:11:45.83 Intake.select :close @14:11:45.98 SSV.dial :exhaust @14:11:46.75 Exhaust.select :open @14:11:46.86 SS.seek 0ml @14:11:55.66 Exhaust.select :close @14:11:55.77 SSV.dial CBV @14:11:55.79
CBV.dial SSV @14:11:56.57 Allowing 130s for 25.0ml of remaining 425.0ml @14:11:56.58 Intake.select :open @14:11:58.74 Intake @16.1psia, vacuum from 10.0 to 13.0psi @14:11:58.75 SS.reconfigure :inhaling 0.38ml/s @14:11:58.80 SS.seek 25ml @14:13:07.58 Stroke completed -- waiting for pressure to equalize ... @14:13:07.59 SS.reconfigure :exhale @14:13:07.64 SS.hold @14:13:36.67 Intake.select :close @14:13:36.82 SSV.dial :exhaust @14:13:37.58 Exhaust.select :open @14:13:37.69 SS.seek 0ml @14:13:46.77 Exhaust.select :close @14:13:46.87 SSV.dial CBV @14:13:46.89
CBV.dial SSV @14:13:47.69 Allowing 130s for 25.0ml of remaining 400.0ml @14:13:47.70 Intake.select :open @14:13:49.87 Intake @16.2psia, vacuum from 10.0 to 13.0psi @14:13:49.88 SS.reconfigure :inhaling 0.38ml/s @14:13:50.36 SS.seek 25ml @14:14:58.44 Stroke completed -- waiting for pressure to equalize ... @14:14:58.45 SS.reconfigure :exhale @14:14:58.50 SS.hold @14:15:33.16 Intake.select :close @14:15:33.31 SSV.dial :exhaust @14:15:34.08 Exhaust.select :open @14:15:34.57 SS.seek 0ml @14:15:43.37 Exhaust.select :close @14:15:43.49 SSV.dial CBV @14:15:43.53
CBV.dial SSV @14:15:44.25 Allowing 130s for 25.0ml of remaining 375.0ml @14:15:44.26 Intake.select :open @14:15:46.43 Intake @16.2psia, vacuum from 10.0 to 13.0psi @14:15:46.45 SS.reconfigure :inhaling 0.38ml/s @14:15:46.50 SS.seek 25ml @14:16:55.53 Stroke completed -- waiting for pressure to equalize ... @14:16:55.54 SS.reconfigure :exhale @14:16:55.59 SS.hold @14:17:34.35 Intake.select :close @14:17:34.50 SSV.dial :exhaust @14:17:35.25 Exhaust.select :open @14:17:35.37 SS.seek 0ml @14:17:44.34 Exhaust.select :close @14:17:44.45 SSV.dial CBV @14:17:44.47
CBV.dial SSV @14:17:45.25 Allowing 130s for 25.0ml of remaining 350.0ml @14:17:45.26 Intake.select :open @14:17:47.42 Intake @16.3psia, vacuum from 10.0 to 13.0psi @14:17:47.44 SS.reconfigure :inhaling 0.38ml/s @14:17:47.48 SS.seek 25ml @14:18:44.69 Vacuum too high -- waiting up to 40 seconds @14:18:44.70 SS.reconfigure :exhale @14:18:44.76 SS.hold @14:19:03.20 Intake @17.5psia, vacuum from 10.0 to 13.0psi @14:19:03.22 SS.reconfigure :inhaling 0.31ml/s @14:19:03.27 SS.seek 25ml @14:19:17.40 Stroke completed -- waiting for pressure to equalize ... @14:19:17.41 SS.reconfigure :exhale @14:19:17.46 SS.hold @14:19:44.76 Intake.select :close @14:19:44.91 SSV.dial :exhaust @14:19:45.67 Exhaust.select :open @14:19:45.79 SS.seek 0ml @14:19:54.65 Exhaust.select :close @14:19:54.76 SSV.dial CBV @14:19:55.18
CBV.dial SSV @14:19:55.99 Allowing 130s for 25.0ml of remaining 325.0ml @14:19:56.01 Intake.select :open @14:19:58.17 Intake @16.2psia, vacuum from 10.0 to 13.0psi @14:19:58.19 SS.reconfigure :inhaling 0.31ml/s @14:19:58.23 SS.seek 25ml @14:21:26.60 Stroke completed -- waiting for pressure to equalize ... @14:21:26.61 SS.reconfigure :exhale @14:21:26.66 SS.hold @14:22:03.85 Intake.select :close @14:22:04.01 SSV.dial :exhaust @14:22:04.77 Exhaust.select :open @14:22:04.89 SS.seek 0ml @14:22:13.74 Exhaust.select :close @14:22:13.85 SSV.dial CBV @14:22:13.89
CBV.dial SSV @14:22:14.62 Allowing 130s for 25.0ml of remaining 300.0ml @14:22:14.63 Intake.select :open @14:22:16.79 Intake @16.3psia, vacuum from 10.0 to 13.0psi @14:22:16.81 SS.reconfigure :inhaling 0.31ml/s @14:22:16.85 SS.seek 25ml @14:23:44.33 Stroke completed -- waiting for pressure to equalize ... @14:23:44.34 SS.reconfigure :exhale @14:23:44.39 SS.hold @14:24:23.38 Intake.select :close @14:24:23.54 SSV.dial :exhaust @14:24:24.29 Exhaust.select :open @14:24:24.41 SS.seek 0ml @14:24:33.27 Exhaust.select :close @14:24:33.38 SSV.dial CBV @14:24:33.40
CBV.dial SSV @14:24:34.16 Allowing 130s for 25.0ml of remaining 275.0ml @14:24:34.17 Intake.select :open @14:24:36.34 Intake @16.1psia, vacuum from 10.0 to 13.0psi @14:24:36.35 SS.reconfigure :inhaling 0.31ml/s @14:24:36.40 SS.seek 25ml @14:26:04.89 Stroke completed -- waiting for pressure to equalize ... @14:26:04.90 SS.reconfigure :exhale @14:26:04.95 SS.hold @14:26:44.18 SS.reconfigure :default @14:26:44.23 SS.hold @14:26:44.29 Intake.select :close @14:26:44.45 ShallowSampler::Clogged in MAIN -- Filtering too Slowly after 750.0ml @14:26:44.46 SSV.dial :exhaust @14:26:45.22 Exhaust.select :open @14:26:45.33 SS.reconfigure :exhale @14:26:45.38 SS.seek 0ml @14:26:54.20 Exhaust.select :close @14:26:54.32 Sampled 750.0ml @14:26:54.33 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @14:26:54.34 Intake.select :close @14:26:54.41 CSV.dial :waste @14:26:54.57 SSV.dial CBV,avoiding: :exhaust @14:26:54.59 CS.seek 0ml @14:26:54.63
CBV.dial SSV @14:26:54.79 CSV.dial :air @14:26:55.54 CS.seek 10ml @14:26:56.57
CTV.dial :air @14:26:57.35 delay 15 seconds @14:27:12.37 SSV.dial :spit,avoiding: :exhaust @14:27:13.68 CTV.dial CSV,avoiding: :intake @14:27:13.71
SS.seek 0ml @14:27:13.83 SSV.dial CBV,avoiding: :exhaust @14:27:19.97 SSV.dial between CBV and plug @14:27:20.46 CBV.dial between SSV and waste2 @14:27:20.84 CSV.dial between air and CTV @14:27:21.59 CS.seek 8.334ml @14:27:26.20 CTV.dial CSV,avoiding: :air @14:27:26.22
CSV.dial CTV @14:27:26.58 CS.reconfigure :pressurize @14:27:26.63 CS.seek 0ml @14:27:40.23 BubblePoint: Intake @24.2psia, Outlet @0.9psia @14:27:40.24 CBV.dial SSV @14:27:40.57 SS.reconfigure :default @14:27:40.62 SS.seek 10ml @14:27:46.20 delay 15 seconds @14:28:01.34 Evacuated: Intake @23.7psia, Outlet @23.3psia @14:28:01.35 SSV.dial :spit,avoiding: :exhaust @14:28:02.40 SS.reconfigure :exhale @14:28:02.45 SS.seek 0ml @14:28:07.48 SS.reconfigure :fast @14:28:07.53 SS.seek 5ml @14:28:10.34 SSV.dial CBV,avoiding: :exhaust @14:28:10.36
CBV.dial SSV @14:28:11.69 CTV.dial :air,avoiding: :intake @14:28:13.03 CSV.dial CTV @14:28:13.09
SS.seek 10ml @14:28:14.24 CSV.dial :air @14:28:14.69 CS.reconfigure :fast @14:28:14.74 CS.seek 0ml @14:28:15.58
SSV.dial :spit,avoiding: :exhaust @14:28:16.90 SS.reconfigure :exhale @14:28:16.95 SS.seek 0ml @14:28:21.98 SS.reconfigure :fast @14:28:22.03 SS.seek 5ml @14:28:23.12 CS.stop @14:28:24.84 SSV.dial CBV,avoiding: :exhaust @14:28:24.86
CBV.dial SSV @14:28:27.20 SSV.dial :spit,avoiding: :exhaust @14:28:28.51 SS.reconfigure :default @14:28:28.55 SS.seek 0ml @14:28:31.81 SS.stop @14:28:31.85 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @14:28:31.86 Intake.select :close @14:28:31.93 CSV.dial :waste @14:28:32.05 SSV.dial CBV,avoiding: :exhaust @14:28:32.07
CBV.dial SSV @14:28:32.72 CS.seek 0ml @14:28:32.78 CSV.dial :air @14:28:33.41
CTV.dial :air @14:28:33.48 delay 15 seconds @14:28:33.53 CS.seek 10ml @14:28:48.51
SSV.dial :spit,avoiding: :exhaust @14:28:49.85 CTV.dial CSV,avoiding: :intake @14:28:50.27
SS.reconfigure :exhale @14:28:50.42 SS.seek 0ml @14:28:50.78 SSV.dial CBV,avoiding: :exhaust @14:28:57.91 SSV.dial between CBV and plug @14:28:58.38 CBV.dial between SSV and waste2 @14:28:58.76 CSV.dial between air and CTV @14:28:59.09 CS.seek 7.33ml @14:29:05.94 CTV.dial CSV,avoiding: :air @14:29:05.97
CSV.dial CTV @14:29:06.32 CS.reconfigure :pressurize @14:29:06.37 CS.seek 0ml @14:29:18.66 BubblePoint: Intake @24.2psia, Outlet @3.8psia @14:29:18.67 CBV.dial SSV @14:29:19.00 SS.reconfigure :default @14:29:19.05 SS.seek 10ml @14:29:24.69 delay 15 seconds @14:29:39.82 Evacuated: Intake @23.9psia, Outlet @23.5psia @14:29:39.83 SSV.dial :spit,avoiding: :exhaust @14:29:40.86 SS.reconfigure :exhale @14:29:40.91 SS.seek 0ml @14:29:45.95 SS.reconfigure :fast @14:29:45.99 SS.seek 5ml @14:29:48.83 SSV.dial CBV,avoiding: :exhaust @14:29:48.86
CBV.dial SSV @14:29:50.21 CTV.dial :air,avoiding: :intake @14:29:51.53 CSV.dial CTV @14:29:51.61
SS.seek 10ml @14:29:52.82 CSV.dial :air @14:29:53.25 CS.reconfigure :fast @14:29:53.30 CS.seek 0ml @14:29:54.08
SSV.dial :spit,avoiding: :exhaust @14:29:55.40 SS.reconfigure :exhale @14:29:55.44 SS.seek 0ml @14:30:00.40 CS.stop @14:30:00.46
SS.reconfigure :fast @14:30:00.51 SS.seek 5ml @14:30:03.29 SSV.dial CBV,avoiding: :exhaust @14:30:03.31
CBV.dial SSV @14:30:05.66 SSV.dial :spit,avoiding: :exhaust @14:30:06.99 SS.reconfigure :default @14:30:07.04 SS.seek 0ml @14:30:10.25 SS.stop @14:30:10.29 Lysing: Adding 2.0ml AqMeOH @14:30:10.30 CBV.dial between CSV and waste1,avoiding: :DBV @14:30:12.25 CTV.dial CSV @14:30:13.58 CSV.dial CSR @14:30:14.37 Pulling 1ml of CSR.AqMeOH @14:30:14.38 CSR.select :AqMeOH @14:30:14.49 CS.reconfigure :default @14:30:14.54 CS.seek 1ml @14:30:18.18 delay 5 seconds @14:30:23.20 CSR.select :air @14:30:23.37 CS.seek 6.001ml @14:30:37.09 delay 2 seconds @14:30:39.11 CSV.dial :waste2 @14:30:39.55 CS.reconfigure :fast @14:30:39.60 CS.seek 0ml @14:30:53.90 CSV.dial CSR @14:30:54.36 Pulling 2ml of CSR.AqMeOH CSR.select :AqMeOH @14:30:54.48 CS.reconfigure :default @14:30:54.53 CS.seek 2ml @14:31:00.47 delay 5 seconds @14:31:05.48 CSR.select :air @14:31:05.65 CS.seek 5ml @14:31:14.12 delay 2 seconds @14:31:16.14 CSV.dial CTV @14:31:16.60 CS.seek 1ml @14:31:28.13 Before lysing Collection Puck Heater at 13C/31.2psia @14:31:28.15 Heating Puck.from 6, :sh1 in Collection Clamp to 70C for 10:00 @14:31:28.17 CH.seek 70C,8:00 @14:33:44.81 CH.hold 10:00,70C @14:33:45.01 Begun lysing Collection Puck Heater at 68.1C/37.9psia @14:43:45.02 Ended lysing Collection Puck Heater at 70C/36.9psia @14:43:45.04 CS.seek 0ml @14:43:48.40 CTV.dial between spare and air,avoiding: :intake @14:43:49.41 CSV.dial :air @14:43:49.85 CS.seek 5ml @14:44:03.45 CSV.dial CTV @14:44:03.91 delay 2 seconds @14:44:05.92 CSV.dial :air @14:44:06.84 delay 2 seconds @14:44:08.85 CSV.dial CTV @14:44:09.31 delay 2 seconds @14:44:11.33 CSV.dial :air @14:44:11.78 delay 2 seconds @14:44:13.81 CS.seek 0ml @14:44:27.66 Puck @82.7psia after final lysis pressurization @14:44:27.71 Recovering Lysate into Collection syringe @14:44:27.74 CSV.dial CBV @14:44:28.78 CBV.dial CSV @14:44:29.17 CS.seek 5ml @14:44:42.98 delay 5 seconds @14:44:48.03 CTV.dial :air @14:44:48.56 CS.seek 7ml @14:44:54.45 delay 5 seconds @14:44:59.47 CSV.dial :air2 @14:44:59.93 CS.seek 10ml @14:45:08.48 CSV.dial between air and CTV,avoiding: CBV @14:45:09.64 Lysing Complete @14:45:09.71 Storing sh1 puck in Collection Clamp to tube 5 @14:45:09.89 SC.reconfigure :tight @14:45:09.93 SC.seek 5 @14:45:13.51 SC.configure :limp @14:45:13.77 Elbow.reconfigure :tight @14:45:13.82 Elbow.seek 5 @14:45:15.58 Forearm.reconfigure :tight @14:45:15.63 Forearm.seek 5 + 450 counts @14:45:16.66 SE.reconfigure :pick @14:45:16.72 SE.seek :beamBreak @14:45:17.73 SE.configure :default @14:45:17.89 Elbow.reconfigure :limp @14:45:17.94 Forearm.reconfigure :loose @14:45:17.99 Forearm.seek 5 @14:45:18.22 Elbow.hold @14:45:18.28 Elbow.reconfigure :tight @14:45:18.38 SE.seek 31.12 Pucks below Top Plate @14:45:18.89 Forearm.force -20 @14:45:18.94 Forearm.seek 5 + 320 counts @14:45:19.67 Forearm.reconfigure :tight @14:45:19.72 Forearm.seek 5 + 450 counts @14:45:19.91 SE.seek 32.12 Pucks below Top Plate @14:45:20.44 Forearm.reconfigure :loose @14:45:20.49 Forearm.seek :retract @14:45:21.42 Elbow.seek CC @14:45:23.12 Elbow.reconfigure :loose @14:45:23.22 Forearm.seek CC @14:45:24.17 Hand.close @14:45:24.83 CC.seek :open @14:45:29.56 Forearm.seek :retract @14:45:30.58 Elbow.reconfigure :tight @14:45:30.63 Elbow.seek 5 @14:45:32.80 Elbow.reconfigure :limp @14:45:32.85 Forearm.seek 5 @14:45:33.92 Elbow.hold @14:45:33.98 Elbow.reconfigure :tight @14:45:34.03 Hand.open @14:45:34.82 Forearm.force -20 @14:45:34.87 Forearm.seek 5 + 312 counts @14:45:35.20 Forearm.reconfigure :tight @14:45:35.25 Forearm.seek 5 + 450 counts @14:45:35.50 SE.seek :down @14:45:36.05 SE.stop @14:45:36.63
Forearm.reconfigure :loose @14:45:36.68 Forearm.seek :retract @14:45:37.66 Tranferring DA @14:45:37.67 Beginning samplePassC @14:45:37.68 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:45:38.31 Elbow.seek FlushPuck::Garage @14:45:40.18 Elbow.reconfigure :loose @14:45:40.28 Forearm.seek :garage @14:45:41.30 Hand.close @14:45:42.07 Forearm.seek :retract @14:45:43.06 Elbow.reconfigure :tight @14:45:43.11 Elbow.seek CC @14:45:46.01 Elbow.reconfigure :loose @14:45:46.11 Forearm.reconfigure :tight @14:45:46.16 Forearm.seek CC @14:45:47.38 CC.seek :closed @14:45:51.90 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.43 @14:45:51.97 Hand.open @14:45:52.64 Forearm.reconfigure :loose @14:45:52.68 Forearm.seek :retract @14:45:53.65 PSV.dial PSR @14:45:55.02 Pulling 1.5ml of PSR.flush @14:45:55.03 PSR.select :flush @14:45:55.16 PS.seek 1.5ml @14:46:11.27 delay 5 seconds @14:46:16.29 PSV.dial :waste1 @14:46:16.76 PS.seek 0ml @14:46:32.98 PSV.dial PSR @14:46:33.45 PSR.select :air @14:46:33.57 PS.seek 2.5ml @14:46:59.99 PRV.dial CBV,avoiding: :ab2 @14:47:00.01
CBV.dial PRV @14:47:02.22 PSV.dial :mixloop @14:47:03.27 CTV.dial :air,avoiding: :intake @14:47:03.50 PTV.dial :PRVmixing @14:47:04.84 PS.seek 0ml @14:47:31.23 PSV.dial :air @14:47:31.71 PS.seek 2.5ml @14:47:58.23 PSV.dial :mixloop @14:47:58.71 PS.seek 0ml @14:48:25.23 PSV.dial :air @14:48:25.71 PS.seek 2.5ml @14:48:52.05 PSV.dial :mixloop @14:48:52.52 PS.seek 0ml @14:49:19.02 Garaging FlushPuck in Collection Clamp @14:49:19.09 Elbow.reconfigure :tight @14:49:19.14 Elbow.seek CC @14:49:19.44 Elbow.reconfigure :loose @14:49:19.54 Forearm.seek CC @14:49:20.56 Hand.close @14:49:21.20 CC.seek :open @14:49:25.85 Forearm.seek :retract @14:49:26.84 Elbow.reconfigure :tight @14:49:26.89 Elbow.seek :garage @14:49:29.79 Elbow.reconfigure :loose @14:49:29.89 Forearm.reconfigure :tight @14:49:29.93 Forearm.seek :garage @14:49:30.98 Hand.open @14:49:31.63 Forearm.reconfigure :loose @14:49:31.68 Forearm.seek :retract @14:49:32.68 PSV.dial :mixloop @14:49:32.76 PTV.dial :PRVmixing @14:49:32.84 PRV.dial CBV @14:49:32.88
CBV.dial PRV @14:49:33.02 CTV.dial :air,avoiding: :intake @14:49:33.04 Loading lyfil puck from tube 7 into Collection Clamp @14:49:33.76 SC.reconfigure :tight @14:49:33.81 SC.seek 7 @14:49:40.55 SC.configure :limp @14:49:40.77 Elbow.reconfigure :tight @14:49:40.82 Elbow.seek 7 @14:49:42.74 Forearm.reconfigure :tight @14:49:42.79 Forearm.seek 7 + 450 counts @14:49:43.83 SE.reconfigure :pick @14:49:43.93 SE.seek :beamBreak @14:49:45.01 SE.configure :default @14:49:45.17 Elbow.seek 7 @14:49:45.35 Forearm.seek 7 + 300 counts @14:49:45.56 Forearm.reconfigure :loose @14:49:45.61 Hand.close @14:49:46.21 SE.seek :down @14:49:46.41
Forearm.seek :retract @14:49:46.94 SE.stop @14:49:47.37
Elbow.seek CC @14:49:48.99 Elbow.reconfigure :loose @14:49:49.09 Forearm.reconfigure :tight @14:49:49.14 Forearm.seek CC @14:49:50.41 CC.seek :closed @14:49:54.95 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.16 @14:49:54.97 Hand.open @14:49:55.64 Forearm.reconfigure :loose @14:49:55.69 Forearm.seek :retract @14:49:56.65 CTV.dial CSV,avoiding: :intake @14:49:57.98 CSV.dial CTV @14:49:58.31 PS.seek 2.5ml @14:50:24.76 CS.seek 4.5ml @14:50:39.70 delay 8 seconds @14:50:47.72 PSV.dial :air @14:50:48.19 delay 2 seconds @14:50:50.20 PSV.dial :waste2 @14:50:51.07 PS.seek 0ml @14:51:17.64 CBV.dial between PRV and waste1 @14:51:17.96 CSV.dial :air @14:51:18.44 CS.seek 10ml @14:51:33.32 CSV.dial CTV @14:51:33.78 CS.seek 0ml @14:52:00.16 CBV.dial :waste1 @14:52:00.48 delay 5 seconds @14:52:05.50 CTV.dial :air,avoiding: :intake @14:52:06.91 Storing lyfil puck in Collection Clamp to tube 6 @14:52:07.09 SC.reconfigure :tight @14:52:07.13 SC.seek 6 @14:52:10.70 SC.configure :limp @14:52:10.87 SE.reconfigure :place @14:52:10.95
Elbow.reconfigure :tight @14:52:10.98 SE.seek :beamBreak @14:52:11.01
Elbow.seek CC @14:52:11.30 Elbow.reconfigure :loose @14:52:11.40 Forearm.seek CC @14:52:12.44 Hand.close @14:52:13.07 CC.seek :open @14:52:13.85 SE.configure :default @14:52:13.90 SE.seek :place @14:52:17.81
Forearm.seek :retract @14:52:18.85 Elbow.reconfigure :tight @14:52:18.90 Elbow.seek 6 @14:52:20.62 Elbow.reconfigure :limp @14:52:20.68 Forearm.seek 6 @14:52:21.66 Elbow.hold @14:52:21.73 Elbow.reconfigure :tight @14:52:21.78 Hand.open @14:52:22.57 Forearm.force -20 @14:52:22.62 Forearm.seek 6 + 296 counts @14:52:22.91 Forearm.reconfigure :tight @14:52:22.96 Forearm.seek 6 + 450 counts @14:52:23.22 SE.seek :down @14:52:24.35
Forearm.reconfigure :loose @14:52:24.40 Forearm.seek :retract @14:52:25.91 SE.stop @14:52:25.95
PSV.dial :air @14:52:26.83 PS.seek 2.5ml @14:52:53.17 delay 1 second @14:52:54.18 PSV.dial :mixloop @14:52:54.67 delay 1 second @14:52:55.68 PSV.dial :air @14:52:56.15 delay 1 second @14:52:57.17 PSV.dial :mixloop @14:52:57.64 delay 1 second @14:52:58.65 PSV.dial :air @14:52:59.12 delay 1 second @14:53:00.14 PS.seek 0ml @14:53:26.48 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @14:53:26.66 Elbow.seek FlushPuck::Garage @14:53:28.94 Elbow.reconfigure :loose @14:53:29.06 Forearm.seek :garage @14:53:30.07 Hand.close @14:53:30.81 Forearm.seek :retract @14:53:31.81 Elbow.reconfigure :tight @14:53:31.86 Elbow.seek PC @14:53:33.11 Elbow.reconfigure :loose @14:53:33.21 Forearm.reconfigure :tight @14:53:33.26 Forearm.seek PC @14:53:34.56 PC.seek :closed @14:53:39.23 Processing Clamp onSpring/offSpring for 16/26 samples, on/off torque 1.66 @14:53:39.30 Hand.open @14:53:40.00 Forearm.reconfigure :loose @14:53:40.05 Forearm.seek :retract @14:53:41.05 PRV.dial :air @14:53:41.51 PSV.dial :mixloop @14:53:42.05 PS.seek 0.3ml @14:53:45.98 PSV.dial PSR @14:53:47.16 Pulling 0.4ml of PSR.air @14:53:47.17 PSR.select :air @14:53:47.19 PS.seek 0.7ml @14:53:52.13 delay 3 seconds @14:53:55.16 PSR.select :air @14:53:55.18 PS.seek 2.5ml @14:54:14.45 delay 3 seconds @14:54:17.46 PSV.dial :mixloop @14:54:18.51 PTV.dial :mixing @14:54:19.26 PBV.dial :waste2 @14:54:20.23 PS.seek 0ml @14:54:46.67 Garaging FlushPuck in Processing Clamp @14:54:47.17 Elbow.reconfigure :tight @14:54:47.24 Elbow.seek PC @14:54:47.36 Elbow.reconfigure :loose @14:54:47.46 Forearm.seek PC @14:54:48.43 Hand.close @14:54:49.07 PC.seek :open @14:54:53.78 Forearm.seek :retract @14:54:54.76 Elbow.reconfigure :tight @14:54:54.81 Elbow.seek :garage @14:54:56.16 Elbow.reconfigure :loose @14:54:56.26 Forearm.reconfigure :tight @14:54:56.31 Forearm.seek :garage @14:54:57.33 Hand.open @14:54:58.01 Forearm.reconfigure :loose @14:54:58.05 Forearm.seek :retract @14:54:59.02 Loading da puck from tube 7 into Processing Clamp @14:54:59.26 SC.reconfigure :tight @14:54:59.31 SC.seek 7 @14:55:02.86 SC.configure :limp @14:55:03.08 Elbow.reconfigure :tight @14:55:03.12 Elbow.seek 7 @14:55:05.05 Forearm.reconfigure :tight @14:55:05.10 Forearm.seek 7 + 450 counts @14:55:06.13 SE.reconfigure :pick @14:55:06.19 SE.seek :beamBreak @14:55:07.40 SE.configure :default @14:55:07.56 Elbow.seek 7 @14:55:07.74 Forearm.seek 7 + 300 counts @14:55:07.94 Forearm.reconfigure :loose @14:55:07.99 Hand.close @14:55:09.08 SE.seek :down @14:55:09.29
Forearm.seek :retract @14:55:09.91 SE.stop @14:55:10.26
Elbow.seek PC @14:55:12.83 Elbow.reconfigure :loose @14:55:12.93 Forearm.reconfigure :tight @14:55:12.98 Forearm.seek PC @14:55:14.24 PC.seek :closed @14:55:18.90 Processing Clamp onSpring/offSpring for 16/26 samples, on/off torque 1.62 @14:55:18.91 Hand.open @14:55:19.63 Forearm.reconfigure :loose @14:55:19.68 Forearm.seek :retract @14:55:20.66 Heating Puck.from 7, :da in Processing Clamp to 28C for 2:00:00 @14:55:20.68 PH.seek 28C,5:00 @14:56:03.40 PH.hold 2:00:00,28C @14:56:03.45 delay 1:30 @14:57:33.50 PSR.select :air @14:57:33.52 PSV.dial PSR @14:57:34.58 Pulling 0.5ml of PSR.wash @14:57:34.59 PSR.select :wash @14:57:34.71 PS.seek 0.5ml @14:57:40.69 delay 5 seconds @14:57:45.71 PSR.select :air @14:57:45.83 PS.seek 2ml @14:58:02.00 PTV.dial :puck @14:58:02.76 PSV.dial PTV @14:58:03.52 PBV.dial :waste1 @14:58:04.93 PS.seek 1.5ml @14:58:10.88 delay 3 seconds @14:58:13.90 PBV.dial between PSV and waste1 @14:58:14.25 PS.seek 0ml @14:58:30.38 delay 5:00 @15:03:30.40 recoverPSRreagent08 @15:03:30.41 PTV.dial :puck @15:03:30.48 PBV.dial :waste1 @15:03:30.84 delay 30 seconds @15:04:00.87 PBV.dial between PSV and waste1 @15:04:01.23 PSR.select :air @15:04:01.25 PSV.dial PSR @15:04:02.02 PS.reconfigure :fast @15:04:02.07 PS.seek 2.5ml @15:04:25.68 PSV.dial PTV @15:04:26.45 delay 5 seconds @15:04:31.47 PSV.dial PSR @15:04:32.23 delay 5 seconds @15:04:37.26 PSV.dial PTV @15:04:38.09 delay 5 seconds @15:04:43.11 PSV.dial :air @15:04:43.99 PS.seek 0ml @15:05:07.28 PTV.dial :PRVmixing,via: PRV @15:05:08.67 PSV.dial :mixloop @15:05:09.12 PRV.dial :ab1 @15:05:09.88 PS.reconfigure :slow @15:05:09.93 PS.seek 0.15ml @15:05:13.47 delay 2 seconds @15:05:15.49 CBV.dial PRV @15:05:15.51
PRV.dial CBV @15:05:16.08 PS.seek 0.35ml @15:05:20.62 delay 2 seconds @15:05:22.64 CBV.dial between PRV and PSV @15:05:22.95 PRV.dial :air @15:05:23.43 PS.seek 0.9ml @15:05:35.12 PRV.dial :waste2 @15:05:35.88 PS.seek 0.5ml @15:05:44.53 addPMLReagent08 @15:05:44.54 PSV.dial :air @15:05:45.00 PS.reconfigure :default @15:05:45.05 PS.seek 2ml @15:06:01.24 PSV.dial :PML @15:06:01.71 PTV.dial :mixing @15:06:02.47 PBV.dial :waste1 @15:06:02.90 PS.seek 1.85ml @15:06:05.34 PBV.dial between PSV and waste1 @15:06:05.72 PS.seek 0ml @15:06:25.46 delay 2 seconds @15:06:27.48 Beginning samplePassC @15:06:27.50 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @15:06:27.68 Elbow.reconfigure :tight @15:06:27.73 Elbow.seek FlushPuck::Garage @15:06:29.04 Elbow.reconfigure :loose @15:06:29.14 Forearm.seek :garage @15:06:30.17 Hand.close @15:06:30.94 Forearm.seek :retract @15:06:31.89 Elbow.reconfigure :tight @15:06:31.94 Elbow.seek CC @15:06:34.85 Elbow.reconfigure :loose @15:06:34.95 Forearm.reconfigure :tight @15:06:35.00 Forearm.seek CC @15:06:36.73 CC.seek :closed @15:06:41.26 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.38 @15:06:41.32 Hand.open @15:06:42.00 Forearm.reconfigure :loose @15:06:42.04 Forearm.seek :retract @15:06:43.04 PSV.dial PSR @15:06:44.15 Pulling 1.5ml of PSR.flush @15:06:44.16 PSR.select :flush @15:06:44.28 PS.seek 1.5ml @15:07:00.43 delay 5 seconds @15:07:05.45 PSV.dial :waste1 @15:07:05.92 PS.seek 0ml @15:07:22.09 PSV.dial PSR @15:07:22.56 PSR.select :air @15:07:22.68 PS.seek 2.5ml @15:07:48.97 PRV.dial CBV,avoiding: :ab2 @15:07:48.99
CBV.dial PRV @15:07:50.62 PSV.dial :mixloop @15:07:51.67 CTV.dial :air,avoiding: :intake @15:07:51.74 PTV.dial :PRVmixing @15:07:52.56 PS.seek 0ml @15:08:18.87 PSV.dial :air @15:08:19.34 PS.seek 2.5ml @15:08:45.65 PSV.dial :mixloop @15:08:46.13 PS.seek 0ml @15:09:12.46 PSV.dial :air @15:09:12.94 PS.seek 2.5ml @15:09:39.49 PSV.dial :mixloop @15:09:39.97 PS.seek 0ml @15:10:06.37 Garaging FlushPuck in Collection Clamp @15:10:06.49 Elbow.reconfigure :tight @15:10:06.96 Elbow.seek CC @15:10:07.29 Elbow.reconfigure :loose @15:10:07.39 Forearm.seek CC @15:10:08.41 Hand.close @15:10:09.06 CC.seek :open @15:10:13.67 Forearm.seek :retract @15:10:14.72 Elbow.reconfigure :tight @15:10:14.76 Elbow.seek :garage @15:10:17.64 Elbow.reconfigure :loose @15:10:17.74 Forearm.reconfigure :tight @15:10:17.79 Forearm.seek :garage @15:10:18.83 Hand.open @15:10:19.49 Forearm.reconfigure :loose @15:10:19.54 Forearm.seek :retract @15:10:20.56 delayUntil "15:14:27.47" @15:10:20.59 Whole Cell Archive sampling 1000ml @15:10:20.60 Loading wcr puck from tube 7 into Collection Clamp @15:10:20.85 SC.reconfigure :tight @15:10:20.90 SC.seek 7 @15:10:21.01 SC.configure :limp @15:10:21.23 Elbow.reconfigure :tight @15:10:21.28 Elbow.seek 7 @15:10:23.23 Forearm.reconfigure :tight @15:10:23.28 Forearm.seek 7 + 450 counts @15:10:24.31 SE.reconfigure :pick @15:10:24.37 SE.seek :beamBreak @15:10:25.60 SE.configure :default @15:10:25.78 Elbow.seek 7 @15:10:26.42 Forearm.seek 7 + 300 counts @15:10:26.65 Forearm.reconfigure :loose @15:10:26.70 Hand.close @15:10:27.32 SE.seek :down @15:10:27.53 Forearm.seek :retract @15:10:28.19 SE.stop @15:10:28.54 Elbow.seek CC @15:10:30.13 Elbow.reconfigure :loose @15:10:30.23 Forearm.reconfigure :tight @15:10:30.29 Forearm.seek CC @15:10:31.59 CC.seek :closed @15:10:36.11 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.31 @15:10:36.12 Hand.open @15:10:36.75 Forearm.reconfigure :loose @15:10:36.80 Forearm.seek :retract @15:10:37.76 collectSample 1000ml w/bubble point of 35psi @15:10:37.78 Priming 7ml @15:10:37.79 Intake.select :close @15:10:37.80 Exhaust.select :close @15:10:37.81 SS.hold @15:10:38.02 Calibrating pressure sensors @15:10:38.03 CTV.dial :air @15:10:38.12 SSV.dial :spit,avoiding: :exhaust @15:10:38.21 SS.reconfigure :fast @15:10:38.26 SS.seek 5ml @15:10:40.94 SSV.dial CBV @15:10:40.96 CBV.dial SSV @15:10:42.34 delay 3.8 seconds @15:10:46.29 Sensor limits: Intake max 100.5psia; Outlet max 92.5psia @15:10:46.30 SSV.dial :spit @15:10:47.66 SS.seek 0ml @15:10:51.65 SSV.dial CBV @15:10:53.00 CTV.dial :intake @15:10:53.78 SSV.dial CBV @15:10:53.80 CBV.dial SSV @15:10:53.95 Allowing 40s for 7.0ml of remaining 7.0ml @15:10:53.97 Intake.select :open @15:10:56.13 Intake @14.6psia, vacuum from 9.1 to 12.1psi @15:10:56.15 SS.reconfigure :inhaling 2.20ml/s @15:10:56.20 SS.seek 7ml @15:11:02.60 Stroke completed -- waiting for pressure to equalize ... @15:11:02.61 SS.reconfigure :exhale @15:11:02.66 SS.hold @15:11:29.15 Intake.select :close @15:11:29.31 SSV.dial :exhaust @15:11:30.08 Exhaust.select :open @15:11:30.20 SS.seek 0ml @15:11:34.26 Exhaust.select :close @15:11:34.38 Sampling 1000.0ml @15:11:34.39 Intake.select :close @15:11:34.40 Exhaust.select :close @15:11:34.41 SS.reconfigure :default @15:11:34.46 SS.hold @15:11:34.67 CTV.dial :intake @15:11:34.84 SSV.dial CBV @15:11:34.86 CBV.dial SSV @15:11:35.66 Allowing 130s for 25.0ml of remaining 1000.0ml @15:11:35.68 Intake.select :open @15:11:37.84 Intake @15.3psia, vacuum from 9.8 to 12.8psi @15:11:37.86 SS.reconfigure :inhaling 2.20ml/s @15:11:37.91 SS.seek 25ml @15:11:45.85 Vacuum too high -- waiting up to 40 seconds @15:11:45.86 SS.reconfigure :exhale @15:11:45.92 SS.hold @15:11:56.17 Intake @18.9psia, vacuum from 10.0 to 13.0psi @15:11:56.19 SS.reconfigure :inhaling 1.76ml/s @15:11:56.24 SS.seek 25ml @15:11:58.32 Vacuum too high -- waiting up to 40 seconds @15:11:58.33 SS.reconfigure :exhale @15:11:58.39 SS.hold @15:12:00.26 Intake @16.8psia, vacuum from 10.0 to 13.0psi @15:12:00.28 SS.reconfigure :inhaling 1.41ml/s @15:12:00.32 SS.seek 25ml @15:12:06.07 Vacuum too high -- waiting up to 40 seconds @15:12:06.08 SS.reconfigure :exhale @15:12:06.14 SS.hold @15:12:12.86 Intake @20.3psia, vacuum from 10.0 to 13.0psi @15:12:12.88 SS.reconfigure :inhaling 1.13ml/s @15:12:12.93 SS.seek 25ml @15:12:14.66 Vacuum too high -- waiting up to 40 seconds @15:12:14.68 SS.reconfigure :exhale @15:12:14.73 SS.hold @15:12:16.51 Intake @19.7psia, vacuum from 10.0 to 13.0psi @15:12:16.52 SS.reconfigure :inhaling 0.91ml/s @15:12:16.57 SS.seek 25ml @15:12:19.07 Vacuum too high -- waiting up to 40 seconds @15:12:19.08 SS.reconfigure :exhale @15:12:19.14 SS.hold @15:12:20.85 Intake @19.0psia, vacuum from 10.0 to 13.0psi @15:12:20.87 SS.reconfigure :inhaling 0.73ml/s @15:12:20.92 SS.seek 25ml @15:12:23.56 Stroke completed -- waiting for pressure to equalize ... @15:12:23.57 SS.reconfigure :exhale @15:12:23.62 SS.hold @15:12:54.56 Intake.select :close @15:12:54.71 SSV.dial :exhaust @15:12:55.50 Exhaust.select :open @15:12:55.61 SS.seek 0ml @15:13:04.50 Exhaust.select :close @15:13:05.06 SSV.dial CBV @15:13:05.11 CBV.dial SSV @15:13:05.86 Allowing 130s for 25.0ml of remaining 975.0ml @15:13:05.87 Intake.select :open @15:13:08.04 Intake @15.9psia, vacuum from 10.0 to 13.0psi @15:13:08.06 SS.reconfigure :inhaling 0.73ml/s @15:13:08.11 SS.seek 25ml @15:13:44.77 Stroke completed -- waiting for pressure to equalize ... @15:13:44.79 SS.reconfigure :exhale @15:13:44.84 SS.hold @15:14:20.03 Intake.select :close @15:14:20.19 SSV.dial :exhaust @15:14:20.96 Exhaust.select :open @15:14:21.08 SS.seek 0ml @15:14:27.48 recoverPRVreagent08 @15:14:27.50 PTV.dial :mixing @15:14:28.25 PBV.dial :waste1 @15:14:28.63 delay 30 seconds @15:14:29.96 Exhaust.select :close @15:14:30.07 SSV.dial CBV @15:14:30.09 CBV.dial SSV @15:14:30.87 Allowing 130s for 25.0ml of remaining 950.0ml @15:14:30.88 Intake.select :open @15:14:33.06 Intake @15.9psia, vacuum from 10.0 to 13.0psi @15:14:33.07 SS.reconfigure :inhaling 0.73ml/s @15:14:33.12 SS.seek 25ml @15:14:58.65 PBV.dial between PSV and waste1 @15:14:59.00 PSV.dial :air @15:14:59.48 PS.reconfigure :fast @15:14:59.53 PS.seek 2.5ml @15:15:09.77 Stroke completed -- waiting for pressure to equalize ... @15:15:09.79 SS.reconfigure :exhale @15:15:09.83 SS.hold @15:15:23.42 PSV.dial :mixloop @15:15:23.91 delay 2 seconds @15:15:25.93 PSV.dial :air @15:15:26.53 delay 2 seconds @15:15:28.54 PSV.dial :mixloop @15:15:29.00 delay 2 seconds @15:15:31.02 PSV.dial :air @15:15:31.62 PS.seek 0ml @15:15:43.18 Intake.select :close @15:15:43.34 SSV.dial :exhaust @15:15:44.10 Exhaust.select :open @15:15:44.22 SS.seek 0ml @15:15:52.98 Exhaust.select :close @15:15:53.08 SSV.dial CBV @15:15:53.10 CBV.dial SSV @15:15:54.34 Allowing 130s for 25.0ml of remaining 925.0ml @15:15:54.35 Intake.select :open @15:15:55.17 washCycleDA #1 of 2 @15:15:55.18 PSR.select :air @15:15:55.20 PSV.dial PSR @15:15:56.52 Intake @15.9psia, vacuum from 10.0 to 13.0psi @15:15:56.54 SS.reconfigure :inhaling 0.73ml/s @15:15:56.59 SS.seek 25ml @15:15:56.67 Pulling 0.5ml of PSR.wash @15:15:56.68 PSR.select :wash @15:15:56.80 PS.reconfigure :default @15:15:56.85 PS.seek 0.5ml @15:16:02.95 delay 5 seconds @15:16:07.97 PSR.select :air @15:16:08.10 PS.seek 2ml @15:16:24.33 PTV.dial :puck @15:16:25.08 PSV.dial PTV @15:16:25.83 PBV.dial :waste1 @15:16:26.25 PS.seek 1.5ml @15:16:32.17 delay 3 seconds @15:16:33.30 Stroke completed -- waiting for pressure to equalize ... @15:16:33.32 SS.reconfigure :exhale @15:16:33.36 SS.hold @15:16:35.19 PBV.dial between PSV and waste1 @15:16:35.55 PS.seek 0ml @15:16:51.67 delay 2:00 @15:17:02.42 Intake.select :close @15:17:02.58 SSV.dial :exhaust @15:17:03.35 Exhaust.select :open @15:17:03.47 SS.seek 0ml @15:17:12.35 Exhaust.select :close @15:17:12.47 SSV.dial CBV @15:17:12.49 CBV.dial SSV @15:17:13.27 Allowing 130s for 25.0ml of remaining 900.0ml @15:17:13.29 Intake.select :open @15:17:15.46 Intake @16.0psia, vacuum from 10.0 to 13.0psi @15:17:15.48 SS.reconfigure :inhaling 0.73ml/s @15:17:15.52 SS.seek 25ml @15:17:49.72 Vacuum too high -- waiting up to 40 seconds @15:17:49.73 SS.reconfigure :exhale @15:17:49.79 SS.hold @15:17:58.52 Intake @17.1psia, vacuum from 10.0 to 13.0psi @15:17:58.53 SS.reconfigure :inhaling 0.59ml/s @15:17:58.58 SS.seek 25ml @15:18:01.50 Stroke completed -- waiting for pressure to equalize ... @15:18:01.52 SS.reconfigure :exhale @15:18:01.56 SS.hold @15:18:22.07 Intake.select :close @15:18:22.23 SSV.dial :exhaust @15:18:23.00 Exhaust.select :open @15:18:23.12 SS.seek 0ml @15:18:32.05 Exhaust.select :close @15:18:32.17 SSV.dial CBV @15:18:32.19 CBV.dial SSV @15:18:32.96 Allowing 130s for 25.0ml of remaining 875.0ml @15:18:32.98 Intake.select :open @15:18:35.14 Intake @16.0psia, vacuum from 10.0 to 13.0psi @15:18:35.16 SS.reconfigure :inhaling 0.59ml/s @15:18:35.21 SS.seek 25ml @15:18:51.69 recoverPSRreagent08 @15:18:51.71 PTV.dial :puck @15:18:51.78 PBV.dial :waste1 @15:18:52.15 delay 30 seconds @15:19:19.67 Stroke completed -- waiting for pressure to equalize ... @15:19:19.69 SS.reconfigure :exhale @15:19:19.74 SS.hold @15:19:22.23 PBV.dial between PSV and waste1 @15:19:22.61 PSR.select :air @15:19:22.63 PSV.dial PSR @15:19:23.39 PS.reconfigure :fast @15:19:23.44 PS.seek 2.5ml @15:19:44.40 Intake.select :close @15:19:44.56 SSV.dial :exhaust @15:19:45.33 Exhaust.select :open @15:19:45.45 SS.seek 0ml @15:19:47.52 PSV.dial PTV @15:19:48.40 delay 5 seconds @15:19:53.43 PSV.dial PSR @15:19:54.17 delay 5 seconds @15:19:54.22 Exhaust.select :close @15:19:54.34 SSV.dial CBV @15:19:54.36 CBV.dial SSV @15:19:55.13 Allowing 130s for 25.0ml of remaining 850.0ml @15:19:55.15 Intake.select :open @15:19:57.31 Intake @16.0psia, vacuum from 10.0 to 13.0psi @15:19:57.33 SS.reconfigure :inhaling 0.59ml/s @15:19:57.38 SS.seek 25ml @15:19:59.19 PSV.dial PTV @15:19:59.95 delay 5 seconds @15:20:04.97 PSV.dial :air @15:20:05.74 PS.seek 0ml @15:20:29.07 washCycleDA #2 of 2 @15:20:29.08 PSR.select :air @15:20:29.10 PSV.dial PSR @15:20:30.45 Pulling 0.5ml of PSR.wash @15:20:30.46 PSR.select :wash @15:20:30.57 PS.reconfigure :default @15:20:30.62 PS.seek 0.5ml @15:20:36.64 delay 5 seconds @15:20:42.05 Stroke completed -- waiting for pressure to equalize ... @15:20:42.06 SS.reconfigure :exhale @15:20:42.11 PSR.select :air @15:20:42.13 SS.hold @15:20:42.38 PS.seek 2ml @15:20:58.57 PTV.dial :puck @15:20:58.65 PSV.dial PTV @15:20:59.40 PBV.dial :waste1 @15:20:59.89 PS.seek 1.5ml @15:21:05.79 delay 3 seconds @15:21:07.10 Intake.select :close @15:21:07.26 SSV.dial :exhaust @15:21:08.05 Exhaust.select :open @15:21:08.17 SS.seek 0ml @15:21:08.81 PBV.dial between PSV and waste1 @15:21:09.19 PS.seek 0ml @15:21:17.02 Exhaust.select :close @15:21:17.13 SSV.dial CBV @15:21:17.15 CBV.dial SSV @15:21:17.94 Allowing 130s for 25.0ml of remaining 825.0ml @15:21:17.96 Intake.select :open @15:21:20.12 Intake @16.0psia, vacuum from 10.0 to 13.0psi @15:21:20.14 SS.reconfigure :inhaling 0.59ml/s @15:21:20.19 SS.seek 25ml @15:21:25.36 delay 2:00 @15:22:04.98 Stroke completed -- waiting for pressure to equalize ... @15:22:05.00 SS.reconfigure :exhale @15:22:05.04 SS.hold @15:22:30.06 Intake.select :close @15:22:30.22 SSV.dial :exhaust @15:22:30.98 Exhaust.select :open @15:22:31.10 SS.seek 0ml @15:22:39.87 Exhaust.select :close @15:22:39.98 SSV.dial CBV @15:22:40.02 CBV.dial SSV @15:22:40.78 Allowing 130s for 25.0ml of remaining 800.0ml @15:22:40.80 Intake.select :open @15:22:42.96 Intake @16.1psia, vacuum from 10.0 to 13.0psi @15:22:42.98 SS.reconfigure :inhaling 0.59ml/s @15:22:43.03 SS.seek 25ml @15:23:25.38 recoverPSRreagent08 @15:23:25.40 PTV.dial :puck @15:23:25.52 PBV.dial :waste1 @15:23:25.90 delay 30 seconds @15:23:28.00 Stroke completed -- waiting for pressure to equalize ... @15:23:28.01 SS.reconfigure :exhale @15:23:28.06 SS.hold @15:23:54.71 Intake.select :close @15:23:54.87 SSV.dial :exhaust @15:23:55.64 Exhaust.select :open @15:23:55.76 SS.seek 0ml @15:23:55.92 PBV.dial between PSV and waste1 @15:23:56.29 PSR.select :air @15:23:56.31 PSV.dial PSR @15:23:57.08 PS.reconfigure :fast @15:23:57.13 PS.seek 2.5ml @15:24:04.56 Exhaust.select :close @15:24:04.68 SSV.dial CBV @15:24:04.72 CBV.dial SSV @15:24:05.47 Allowing 130s for 25.0ml of remaining 775.0ml @15:24:05.48 Intake.select :open @15:24:07.65 Intake @16.1psia, vacuum from 10.0 to 13.0psi @15:24:07.67 SS.reconfigure :inhaling 0.59ml/s @15:24:07.72 SS.seek 25ml @15:24:21.07 PSV.dial PTV @15:24:21.84 delay 5 seconds @15:24:26.86 PSV.dial PSR @15:24:27.70 delay 5 seconds @15:24:32.72 PSV.dial PTV @15:24:33.49 delay 5 seconds @15:24:38.51 PSV.dial :air @15:24:39.28 PS.seek 0ml @15:24:52.75 Stroke completed -- waiting for pressure to equalize ... @15:24:52.80 SS.reconfigure :exhale @15:24:53.01 SS.hold @15:25:02.44 getPMLReagent08 @15:25:02.45 PRV.dial :air @15:25:02.97 delay 5 seconds @15:25:07.99 PTV.dial :PRVmixing @15:25:09.32 delay 5 seconds @15:25:14.34 PRV.dial :ab2 @15:25:15.26 PTV.dial :PRVmixing @15:25:15.33 PSV.dial :mixloop @15:25:15.81 PS.reconfigure :default @15:25:15.86 PS.seek 0.3ml @15:25:19.73 delay 2 seconds @15:25:21.76 Intake.select :close @15:25:21.81 PRV.dial :air @15:25:21.92 SSV.dial :exhaust @15:25:22.33 PS.seek 0.55ml @15:25:22.70 Exhaust.select :open @15:25:22.82 SS.seek 0ml @15:25:25.76 addPMLReagent08 @15:25:25.77 PSV.dial :air @15:25:26.25 PS.seek 2ml @15:25:31.64 Exhaust.select :close @15:25:31.75 SSV.dial CBV @15:25:31.78 CBV.dial SSV @15:25:32.54 Allowing 130s for 25.0ml of remaining 750.0ml @15:25:32.56 Intake.select :open @15:25:34.77 Intake @16.1psia, vacuum from 10.0 to 13.0psi @15:25:34.79 SS.reconfigure :inhaling 0.59ml/s @15:25:34.84 SS.seek 25ml @15:25:41.82 PSV.dial :PML @15:25:42.29 PTV.dial :mixing @15:25:43.05 PBV.dial :waste1 @15:25:43.49 PS.seek 1.85ml @15:25:45.85 PBV.dial between PSV and waste1 @15:25:46.21 PS.seek 0ml @15:26:04.30 Vacuum too high -- waiting up to 40 seconds @15:26:04.32 SS.reconfigure :exhale @15:26:04.37 SS.hold @15:26:06.02 delay 2 seconds @15:26:08.06 delay 8:00 @15:26:15.97 Intake @17.4psia, vacuum from 10.0 to 13.0psi @15:26:15.99 SS.reconfigure :inhaling 0.47ml/s @15:26:16.04 SS.seek 25ml @15:26:35.58 Vacuum too high -- waiting up to 40 seconds @15:26:35.60 SS.reconfigure :exhale @15:26:35.65 SS.hold @15:26:42.74 Intake @17.5psia, vacuum from 10.0 to 13.0psi @15:26:42.76 SS.reconfigure :inhaling 0.38ml/s @15:26:42.81 SS.seek 25ml @15:26:42.87 Stroke completed -- waiting for pressure to equalize ... @15:26:42.89 SS.reconfigure :exhale @15:26:42.94 SS.hold @15:26:55.65 Intake.select :close @15:26:55.81 SSV.dial :exhaust @15:26:56.58 Exhaust.select :open @15:26:56.70 SS.seek 0ml @15:27:05.57 Exhaust.select :close @15:27:05.68 SSV.dial CBV @15:27:05.70 CBV.dial SSV @15:27:06.48 Allowing 130s for 25.0ml of remaining 725.0ml @15:27:06.49 Intake.select :open @15:27:08.68 Intake @16.0psia, vacuum from 10.0 to 13.0psi @15:27:08.70 SS.reconfigure :inhaling 0.38ml/s @15:27:08.75 SS.seek 25ml @15:28:17.02 Stroke completed -- waiting for pressure to equalize ... @15:28:17.04 SS.reconfigure :exhale @15:28:17.08 SS.hold @15:28:39.59 Intake.select :close @15:28:39.75 SSV.dial :exhaust @15:28:40.98 Exhaust.select :open @15:28:41.10 SS.seek 0ml @15:28:50.15 Exhaust.select :close @15:28:50.27 SSV.dial CBV @15:28:50.29 CBV.dial SSV @15:28:51.07 Allowing 130s for 25.0ml of remaining 700.0ml @15:28:51.09 Intake.select :open @15:28:53.25 Intake @15.9psia, vacuum from 10.0 to 13.0psi @15:28:53.27 SS.reconfigure :inhaling 0.38ml/s @15:28:53.32 SS.seek 25ml @15:30:01.16 Stroke completed -- waiting for pressure to equalize ... @15:30:01.17 SS.reconfigure :exhale @15:30:01.22 SS.hold @15:30:27.87 Intake.select :close @15:30:28.03 SSV.dial :exhaust @15:30:28.81 Exhaust.select :open @15:30:28.93 SS.seek 0ml @15:30:37.79 Exhaust.select :close @15:30:37.90 SSV.dial CBV @15:30:37.92 CBV.dial SSV @15:30:38.69 Allowing 130s for 25.0ml of remaining 675.0ml @15:30:38.71 Intake.select :open @15:30:40.88 Intake @15.9psia, vacuum from 10.0 to 13.0psi @15:30:40.90 SS.reconfigure :inhaling 0.38ml/s @15:30:40.95 SS.seek 25ml @15:31:49.21 Stroke completed -- waiting for pressure to equalize ... @15:31:49.22 SS.reconfigure :exhale @15:31:49.27 SS.hold @15:32:19.99 Intake.select :close @15:32:20.15 SSV.dial :exhaust @15:32:20.91 Exhaust.select :open @15:32:21.03 SS.seek 0ml @15:32:30.13 Exhaust.select :close @15:32:30.24 SSV.dial CBV @15:32:30.27 CBV.dial SSV @15:32:31.04 Allowing 130s for 25.0ml of remaining 650.0ml @15:32:31.05 Intake.select :open @15:32:33.22 Intake @16.1psia, vacuum from 10.0 to 13.0psi @15:32:33.24 SS.reconfigure :inhaling 0.38ml/s @15:32:33.29 SS.seek 25ml @15:33:41.00 Stroke completed -- waiting for pressure to equalize ... @15:33:41.01 SS.reconfigure :exhale @15:33:41.06 SS.hold @15:34:08.07 recoverPRVreagent08 @15:34:08.09 PTV.dial :mixing @15:34:08.15 PBV.dial :waste1 @15:34:08.51 delay 30 seconds @15:34:18.00 Intake.select :close @15:34:18.16 SSV.dial :exhaust @15:34:18.99 Exhaust.select :open @15:34:19.11 SS.seek 0ml @15:34:27.95 Exhaust.select :close @15:34:28.10 SSV.dial CBV @15:34:28.13 CBV.dial SSV @15:34:28.89 Allowing 130s for 25.0ml of remaining 625.0ml @15:34:28.91 Intake.select :open @15:34:31.08 Intake @16.0psia, vacuum from 10.0 to 13.0psi @15:34:31.10 SS.reconfigure :inhaling 0.38ml/s @15:34:31.15 SS.seek 25ml @15:34:38.53 PBV.dial between PSV and waste1 @15:34:38.89 PSV.dial :air @15:34:39.48 PS.reconfigure :fast @15:34:39.53 PS.seek 2.5ml @15:35:03.43 PSV.dial :mixloop @15:35:03.89 delay 2 seconds @15:35:05.90 PSV.dial :air @15:35:06.37 delay 2 seconds @15:35:08.39 PSV.dial :mixloop @15:35:08.86 delay 2 seconds @15:35:10.88 PSV.dial :air @15:35:11.35 PS.seek 0ml @15:35:34.56 washCycleDA #1 of 3 @15:35:34.57 PSR.select :air @15:35:34.58 PSV.dial PSR @15:35:35.93 Pulling 0.5ml of PSR.wash @15:35:35.94 PSR.select :wash @15:35:36.06 PS.reconfigure :default @15:35:36.11 PS.seek 0.5ml @15:35:37.45 Vacuum too high -- waiting up to 40 seconds @15:35:37.46 SS.reconfigure :exhale @15:35:37.52 SS.hold @15:35:42.09 delay 5 seconds @15:35:47.10 PSR.select :air @15:35:47.22 PS.seek 2ml @15:35:58.56 Intake @17.2psia, vacuum from 10.0 to 13.0psi @15:35:58.58 SS.reconfigure :inhaling 0.31ml/s @15:35:58.62 SS.seek 25ml @15:36:02.17 Stroke completed -- waiting for pressure to equalize ... @15:36:02.18 SS.reconfigure :exhale @15:36:02.23 SS.hold @15:36:03.27 PTV.dial :puck @15:36:04.02 PSV.dial PTV @15:36:04.77 PBV.dial :waste1 @15:36:05.28 PS.seek 1.5ml @15:36:11.30 delay 3 seconds @15:36:14.32 PBV.dial between PSV and waste1 @15:36:14.69 PS.seek 0ml @15:36:24.77 Intake.select :close @15:36:24.93 SSV.dial :exhaust @15:36:25.69 Exhaust.select :open @15:36:25.81 SS.seek 0ml @15:36:31.03 delay 2:00 @15:36:34.66 Exhaust.select :close @15:36:34.77 SSV.dial CBV @15:36:34.79 CBV.dial SSV @15:36:35.56 Allowing 130s for 25.0ml of remaining 600.0ml @15:36:35.58 Intake.select :open @15:36:37.74 Intake @16.0psia, vacuum from 10.0 to 13.0psi @15:36:37.76 SS.reconfigure :inhaling 0.31ml/s @15:36:37.81 SS.seek 25ml @15:38:05.40 Stroke completed -- waiting for pressure to equalize ... @15:38:05.41 SS.reconfigure :exhale @15:38:05.46 SS.hold @15:38:31.05 recoverPSRreagent08 @15:38:31.06 PTV.dial :puck @15:38:31.13 PBV.dial :waste1 @15:38:31.51 delay 30 seconds @15:38:45.01 Intake.select :close @15:38:45.17 SSV.dial :exhaust @15:38:45.93 Exhaust.select :open @15:38:46.05 SS.seek 0ml @15:38:55.11 Exhaust.select :close @15:38:55.22 SSV.dial CBV @15:38:55.24 CBV.dial SSV @15:38:56.01 Allowing 130s for 25.0ml of remaining 575.0ml @15:38:56.03 Intake.select :open @15:38:58.20 Intake @16.1psia, vacuum from 10.0 to 13.0psi @15:38:58.22 SS.reconfigure :inhaling 0.31ml/s @15:38:58.27 SS.seek 25ml @15:39:01.52 PBV.dial between PSV and waste1 @15:39:01.90 PSR.select :air @15:39:01.92 PSV.dial PSR @15:39:02.70 PS.reconfigure :fast @15:39:02.75 PS.seek 2.5ml @15:39:26.64 PSV.dial PTV @15:39:27.41 delay 5 seconds @15:39:32.43 PSV.dial PSR @15:39:33.19 delay 5 seconds @15:39:38.22 PSV.dial PTV @15:39:38.98 delay 5 seconds @15:39:44.00 PSV.dial :air @15:39:44.79 PS.seek 0ml @15:40:07.99 washCycleDA #2 of 3 @15:40:08.00 PSR.select :air @15:40:08.02 PSV.dial PSR @15:40:09.36 Pulling 0.5ml of PSR.wash @15:40:09.37 PSR.select :wash @15:40:09.49 PS.reconfigure :default @15:40:09.54 PS.seek 0.5ml @15:40:15.49 delay 5 seconds @15:40:20.51 PSR.select :air @15:40:20.63 PS.seek 2ml @15:40:25.83 Stroke completed -- waiting for pressure to equalize ... @15:40:25.84 SS.reconfigure :exhale @15:40:25.89 SS.hold @15:40:36.84 PTV.dial :puck @15:40:36.91 PSV.dial PTV @15:40:37.66 PBV.dial :waste1 @15:40:38.10 PS.seek 1.5ml @15:40:44.08 delay 3 seconds @15:40:47.10 PBV.dial between PSV and waste1 @15:40:47.49 PS.seek 0ml @15:41:03.65 delay 2:00 @15:41:06.48 SS.reconfigure :default @15:41:06.61 I2C::Bus::Error in core_reader -- Unexpected ACK byte: 0x43 @15:41:06.89 ShallowSampler::Clogged in MAIN -- Filtering too Slowly @15:41:06.90 Waiting to Resume from Checkpoint "Filtering too Slowly" @15:41:06.92 BadNews.email "Filtering too Slowly",Subject: "ShallowSampler::Clogged in MAIN" @15:41:07.04 Polling Can every 10:00 @15:43:03.68 recoverPSRreagent08 @15:43:03.69 PTV.dial :puck @15:43:03.77 PBV.dial :waste1 @15:43:04.14 delay 30 seconds /suscript/connectNetwork: No such file or directory @15:43:14.40 Errno::ETIMEDOUT in Qmail -- Connection timed out - connect(2) @15:43:14.42 Retry #1 of 10 after 10:00 @15:43:34.17 PBV.dial between PSV and waste1 @15:43:34.57 PSR.select :air @15:43:34.59 PSV.dial PSR @15:43:35.37 PS.reconfigure :fast @15:43:35.42 PS.seek 2.5ml @15:43:59.33 PSV.dial PTV @15:44:00.22 delay 5 seconds @15:44:05.24 PSV.dial PSR @15:44:05.99 delay 5 seconds @15:44:11.01 PSV.dial PTV @15:44:11.77 delay 5 seconds @15:44:16.79 PSV.dial :air @15:44:17.57 PS.seek 0ml @15:44:40.74 washCycleDA #3 of 3 @15:44:40.75 PSR.select :air @15:44:40.77 PSV.dial PSR @15:44:42.11 Pulling 0.5ml of PSR.wash @15:44:42.12 PSR.select :wash @15:44:42.25 PS.reconfigure :default @15:44:42.30 PS.seek 0.5ml @15:44:48.17 delay 5 seconds @15:44:53.19 PSR.select :air @15:44:53.32 PS.seek 2ml @15:45:09.42 PTV.dial :puck @15:45:09.50 PSV.dial PTV @15:45:10.25 PBV.dial :waste1 @15:45:10.69 PS.seek 1.5ml @15:45:16.58 delay 3 seconds @15:45:19.60 PBV.dial between PSV and waste1 @15:45:19.97 PS.seek 0ml @15:45:36.11 delay 2:00 @15:47:36.14 recoverPSRreagent08 @15:47:36.15 PTV.dial :puck @15:47:36.23 PBV.dial :waste1 @15:47:36.61 delay 30 seconds @15:48:06.63 PBV.dial between PSV and waste1 @15:48:07.01 PSR.select :air @15:48:07.03 PSV.dial PSR @15:48:07.80 PS.reconfigure :fast @15:48:07.85 PS.seek 2.5ml @15:48:31.70 PSV.dial PTV @15:48:32.59 delay 5 seconds @15:48:37.61 PSV.dial PSR @15:48:38.36 delay 5 seconds @15:48:43.38 PSV.dial PTV @15:48:44.14 delay 5 seconds @15:48:49.16 PSV.dial :air @15:48:49.92 PS.seek 0ml @15:49:13.04 Gate.power :camera,:ON @15:49:13.06 Mixing DA Substrates @15:49:13.07 PSR.select :air @15:49:13.08 PSV.dial PSR @15:49:14.48 Pulling 0.25ml of PSR.sub1 @15:49:14.51 PSR.select :sub1 @15:49:14.70 PS.reconfigure :default @15:49:14.81 PS.seek 0.25ml @15:49:19.54 delay 5 seconds @15:49:24.56 Pulling 0.25ml of PSR.sub2 @15:49:24.58 PSR.select :sub2 @15:49:24.69 PS.seek 0.5ml @15:49:28.10 delay 5 seconds @15:49:33.12 PSR.select :air @15:49:33.24 PS.seek 2ml @15:49:49.45 PTV.dial :puck @15:49:49.52 PSV.dial PTV @15:49:50.40 PBV.dial :waste1 @15:49:50.84 PS.seek 1.5ml @15:49:56.82 delay 3 seconds @15:49:59.84 PBV.dial between PSV and waste1 @15:50:00.22 PS.seek 0ml @15:50:16.35 delay 10 seconds @15:50:26.38 recoverPSRreagent08 @15:50:26.39 PTV.dial :puck @15:50:26.46 PBV.dial :waste1 @15:50:26.85 delay 30 seconds @15:50:56.88 PBV.dial between PSV and waste1 @15:50:57.26 PSR.select :air @15:50:57.28 PSV.dial PSR @15:50:58.15 PS.reconfigure :fast @15:50:58.20 PS.seek 2.5ml @15:51:22.32 PSV.dial PTV @15:51:23.20 delay 5 seconds @15:51:28.22 PSV.dial PSR @15:51:29.09 delay 5 seconds @15:51:34.11 PSV.dial PTV @15:51:34.98 delay 5 seconds @15:51:40.00 PSV.dial :air @15:51:40.78 PS.seek 0ml @15:52:04.57 Additional air clear @15:52:04.58 PSR.select :air @15:52:04.60 PSV.dial PSR @15:52:05.94 Pulling 2ml of PSR.air @15:52:05.95 PSR.select :air @15:52:05.98 PS.reconfigure :default @15:52:06.02 PS.seek 2ml @15:52:27.26 delay 5 seconds @15:52:32.28 PTV.dial :puck @15:52:32.36 PSV.dial PTV @15:52:33.13 PBV.dial :waste1 @15:52:33.57 PS.seek 1.5ml @15:52:39.54 delay 3 seconds @15:52:42.56 PBV.dial between PSV and waste1 @15:52:42.93 delay 1 second @15:52:43.95 PSV.dial :waste1 @15:52:45.03 PS.seek 0ml @15:53:01.25 Processing Puck Heater at 28.1C before imaging @15:53:01.26 PH.coast @15:53:01.36 Imaging da puck in Processing Clamp while storing it to tube 6 @15:53:01.54 SC.reconfigure :tight @15:53:01.59 SC.seek 6 @15:53:05.17 SC.configure :limp @15:53:05.39 Elbow.reconfigure :tight @15:53:05.87 Elbow.seek 6 @15:53:07.70 Forearm.reconfigure :tight @15:53:07.76 Forearm.seek 6 + 450 counts @15:53:08.82 SE.reconfigure :pick @15:53:08.88 SE.seek :beamBreak @15:53:11.91 SE.configure :default @15:53:12.08 Elbow.reconfigure :limp @15:53:12.13 Forearm.reconfigure :loose @15:53:12.18 Forearm.seek 6 @15:53:12.40 Elbow.hold @15:53:12.46 Elbow.reconfigure :tight @15:53:12.57 SE.seek 0.16 Pucks below Top Plate @15:53:13.01 Forearm.force -20 @15:53:13.05 Forearm.seek 6 + 294 counts @15:53:13.33 Forearm.reconfigure :tight @15:53:13.38 Forearm.seek 6 + 450 counts @15:53:13.72 SE.seek 1.16 Pucks below Top Plate @15:53:14.20 Forearm.reconfigure :loose @15:53:14.25 Forearm.seek :retract @15:53:15.21 Elbow.seek PC @15:53:17.83 Elbow.reconfigure :loose @15:53:18.03 Forearm.seek PC @15:53:19.02 Hand.close @15:53:19.40 upsync @15:53:19.65 PC.seek :open @15:53:24.47 Forearm.seek :retract @15:53:25.52 Elbow.reconfigure :tight @15:53:25.58 Elbow.seek 6 @15:53:28.20 Forearm.reconfigure :tight @15:53:28.25 Forearm.seek 6 + 450 counts @15:53:29.31 Hand.open @15:53:29.96 SE.seek 0.81 Pucks below Top Plate @15:53:30.24 SE.reconfigure :seal @15:53:30.29 SE.seek -0.16 Pucks below Top Plate @15:53:30.85 Lights, camera, ACTION! @15:53:30.89 PuckCamera: snap -bin=2,2 -auto /var/log/esp/da23dec1515h750ml.tif @15:53:50.35 Waiting >20 seconds for da to relinquish FlushPuck @15:53:52.07 SX HX9/16 (USB:29): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 17.875 seconds /var/log/esp/da23dec1515h750ml.tif: TIFF Upload Complete @15:53:52.10 PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da23dec1515h750ml15s.tif @15:54:07.75 SX HX9/16 (USB:29): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15 seconds /var/log/esp/midres/da23dec1515h750ml15s.tif: TIFF Upload Complete @15:54:07.78 PuckCamera: snap -bin=2,2 20.0 /var/log/esp/midres/da23dec1515h750ml20s.tif @15:54:28.39 SX HX9/16 (USB:29): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 20 seconds /var/log/esp/midres/da23dec1515h750ml20s.tif: TIFF Upload Complete @15:54:28.40 Gate.power :camera,:OFF @15:54:28.43 DAfans.email "17.875 sec auto-exposure",Subject: "da23dec1515h750ml images!" @15:54:28.89 Elbow.seek 6 @15:54:29.12 Forearm.seek 6 + 450 counts @15:54:29.29 SE.reconfigure :gently @15:54:29.35 SE.seek 0.56 Pucks below Top Plate @15:54:29.98 Elbow.reconfigure :limp @15:54:30.04 Forearm.reconfigure :loose @15:54:30.11 Forearm.seek 6 @15:54:30.33 Elbow.hold @15:54:30.42 Elbow.reconfigure :tight @15:54:30.48 SE.reconfigure :default @15:54:30.54 SE.seek 0.74 Pucks below Top Plate @15:54:30.99 Forearm.force -20 @15:54:31.19 Forearm.seek 6 + 316 counts @15:54:31.47 Forearm.reconfigure :tight @15:54:31.53 Forearm.seek 6 + 450 counts @15:54:31.82 SE.seek :down @15:54:33.10 Forearm.reconfigure :loose @15:54:34.33 Forearm.seek :retract @15:54:34.57 SE.stop @15:54:35.46 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @15:54:35.87 Elbow.seek FlushPuck::Garage @15:54:37.91 Elbow.reconfigure :loose @15:54:38.12 Forearm.seek :garage @15:54:39.22 Hand.close @15:54:40.15 Forearm.seek :retract @15:54:41.19 Elbow.reconfigure :tight @15:54:41.28 Elbow.seek PC @15:54:42.67 Elbow.reconfigure :loose @15:54:42.87 Forearm.reconfigure :tight @15:54:42.96 Forearm.seek PC @15:54:44.50 PC.seek :closed @15:54:49.44 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.66 @15:54:49.69 Hand.open @15:54:50.62 Forearm.reconfigure :loose @15:54:50.75 Forearm.seek :retract @15:54:51.80 PSR.select :air @15:54:51.84 PSV.dial PSR @15:54:52.37 Pulling 0.5ml of PSR.flush @15:54:52.39 PSR.select :flush @15:54:52.57 PS.seek 0.5ml @15:54:58.53 delay 5 seconds @15:55:03.22 upsync @15:55:03.56 PSR.select :air @15:55:03.68 PS.seek 2.5ml @15:55:24.97 delay 1 second @15:55:26.00 PRV.dial :waste2,avoiding: :ab2 @15:55:27.88 PTV.dial :PRVmixing @15:55:29.22 PSV.dial :mixloop @15:55:30.71 PS.seek :empty @15:55:57.13 PSR.select :air @15:55:57.15 PSV.dial PSR @15:55:58.24 Pulling 0.5ml of PSR.flush @15:55:58.26 PSR.select :flush @15:55:58.47 PS.seek 0.5ml @15:56:04.44 delay 5 seconds @15:56:09.46 PSR.select :air @15:56:09.58 PS.seek 2.5ml @15:56:30.88 delay 1 second @15:56:31.90 PBV.dial :waste1 @15:56:32.27 PTV.dial :mixing @15:56:33.02 PSV.dial :mixloop @15:56:34.08 PS.seek :empty @15:57:00.47 PSV.dial PSR @15:57:01.64 PSR.select :flush @15:57:01.76 PS.seek 1ml @15:57:12.81 PSR.select :air @15:57:12.93 PS.seek 2.5ml @15:57:29.12 PSV.dial PTV @15:57:29.88 PTV.dial :puck @15:57:30.63 PBV.dial :waste1 @15:57:30.71 PS.seek 0ml @15:57:57.09 PSV.dial PSR @15:57:57.86 PSR.select :flush @15:57:57.99 PS.seek 0.5ml @15:58:03.97 PSR.select :air @15:58:04.09 PS.seek 2.5ml @15:58:25.40 PSV.dial PTV @15:58:26.17 PBV.dial PSV @15:58:26.74 PS.seek 0ml @15:58:53.31 PSV.dial PBV @15:58:53.79 PS.seek 2.5ml @15:59:20.18 PSV.dial :waste1 @15:59:20.94 PS.seek 0ml @15:59:47.34 PSV.dial PSR @15:59:47.81 PSR.select :air @15:59:47.84 PS.seek 2.5ml @16:00:14.15 PSV.dial PTV @16:00:15.01 PBV.dial PSV @16:00:15.10 PS.seek 0ml @16:00:41.62 PSV.dial PBV @16:00:42.08 PS.seek 2.5ml @16:01:08.62 PSV.dial :waste1 @16:01:09.38 PS.seek 0ml @16:01:35.71 PSV.dial PSR @16:01:36.56 PSR.select :flush @16:01:36.75 PS.seek 1ml @16:01:47.81 PSR.select :air @16:01:47.93 PS.seek 2.5ml @16:02:04.12 PSV.dial PTV @16:02:04.98 PTV.dial :puck @16:02:05.17 PBV.dial :waste1 @16:02:05.74 PS.seek 0ml @16:02:32.02 PSV.dial PSR @16:02:32.78 PSR.select :flush @16:02:32.90 PS.seek 0.5ml @16:02:38.87 PSR.select :air @16:02:38.99 PS.seek 2.5ml @16:03:00.24 PSV.dial PTV @16:03:01.01 PBV.dial PSV @16:03:01.58 PS.seek 0ml @16:03:27.93 PSV.dial PBV @16:03:28.41 PS.seek 2.5ml @16:03:54.77 PSV.dial :waste1 @16:03:55.53 PS.seek 0ml @16:04:21.87 PSV.dial PSR @16:04:22.34 PSR.select :air @16:04:22.36 PS.seek 2.5ml @16:04:48.74 PSV.dial PTV @16:04:49.60 PBV.dial PSV @16:04:49.69 PS.seek 0ml @16:05:16.27 PSV.dial PBV @16:05:16.73 PS.seek 2.5ml @16:05:43.12 PSV.dial :waste1 @16:05:43.87 PS.seek 0ml @16:06:10.21 Garaging FlushPuck in Processing Clamp @16:06:10.28 Elbow.reconfigure :tight @16:06:10.33 Elbow.seek PC @16:06:10.46 Elbow.reconfigure :loose @16:06:10.56 Forearm.seek PC @16:06:11.51 Hand.close @16:06:12.14 PC.seek :open @16:06:16.85 Forearm.seek :retract @16:06:17.83 Elbow.reconfigure :tight @16:06:17.88 Elbow.seek :garage @16:06:19.61 Elbow.reconfigure :loose @16:06:19.78 Forearm.reconfigure :tight @16:06:19.83 Forearm.seek :garage @16:06:20.85 Hand.open @16:06:21.57 Forearm.reconfigure :loose @16:06:21.62 Forearm.seek :retract @16:37:08.31 Session Begun @16:37:13.93 -> MainThread.resume @16:37:13.95
Resuming Thread after Filtering too Slowly at 15:41:06 # @16:37:14.00 SS.reconfigure :default @16:37:14.09 SS.hold @16:37:14.18 Intake.select :close @16:37:14.36 ShallowSampler::Clogged in MAIN -- Intake @16psia & Outlet @13psia won't equalize after 450.0ml @16:37:14.38 SSV.dial :exhaust @16:37:15.15 Exhaust.select :open @16:37:15.29 SS.reconfigure :exhale @16:37:15.36 SS.seek 0ml @16:37:24.15 Exhaust.select :close @16:37:24.28 Sampled 450.0ml @16:37:24.30 Drying puck before fixing for 5:00 @16:37:24.32 CBV.dial :waste2b @16:37:25.11 delay 1:00 @16:38:25.14 CSV.dial :air @16:38:25.62 CS.reconfigure :fast @16:38:25.69 CS.seek 10ml @16:38:49.91 Pumping wcr puck from tube 7 up to 35psi... @16:38:49.93 CBV.dial SSV @16:38:51.26 CBV.dial between CSV and waste2b @16:38:52.23 CSV.dial between air and CTV @16:38:52.57 CS.seek 7.048ml @16:39:00.04 CTV.dial CSV,avoiding: :air @16:39:00.06
CSV.dial CTV @16:39:00.95 CS.reconfigure :pressurize @16:39:01.02 CS.seek 0ml @16:39:19.56 CSV.dial :air @16:39:20.04 CS.reconfigure :fast @16:39:20.11 CS.seek 10ml @16:39:44.34 CSV.dial between air and CTV @16:39:44.68 CS.seek 4.706ml @16:39:57.60 CTV.dial CSV,avoiding: :air @16:39:57.62
CSV.dial CTV @16:39:58.54 CS.reconfigure :pressurize @16:39:58.61 CS.seek 0ml @16:40:11.25 CSV.dial :air @16:40:11.71 CS.reconfigure :fast @16:40:11.78 CS.seek 10ml @16:40:35.90 CSV.dial between air and CTV @16:40:36.24 CS.seek 4.879ml @16:40:48.57 CTV.dial CSV,avoiding: :air @16:40:48.59
CSV.dial CTV @16:40:48.99 CS.reconfigure :pressurize @16:40:49.06 CS.seek 0ml @16:41:02.19 CSV.dial :air @16:41:02.66 CS.reconfigure :fast @16:41:02.74 CS.seek 10ml @16:41:26.87 CSV.dial between air and CTV @16:41:27.21 CS.seek 4.835ml @16:41:39.66 CTV.dial CSV,avoiding: :air @16:41:39.69
CSV.dial CTV @16:41:40.07 CS.reconfigure :pressurize @16:41:40.14 CS.seek 0ml @16:41:53.09 CSV.dial :air @16:41:53.56 CS.reconfigure :default @16:41:53.63 CS.hold @16:41:53.78 ShallowSampler::Error in MAIN -- Puck.from 7, :wcr reached only 18.5 of 35psi after pumping in 40ml of gas @16:41:53.80 Waiting to Resume from Checkpoint "Pumping wcr puck from tube 7 up to 35psi..." @16:41:53.83 BadNews.email "Puck.from 7, :wcr reached only 18.5 of 35psi after pumping in 40ml of gas",Subject: "ShallowSampler::Error in MAIN" @16:41:58.63 upsync @16:44:20.36 -> Resumq @16:44:20.38 NameError in dave -- uninitialized constant Resumq @16:44:22.89 -> Resume @16:44:22.90 NameError in dave -- uninitialized constant Resume @16:44:39.81 -> Resume from Checkpoint @16:44:39.83 NameError in dave -- uninitialized constant Checkpoint @16:45:17.12 -> MainThread.checkpoint [@14:45:37 Beginning samplePassC, @14:45:37 Loading FlushPuck into Collection Clamp from FlushPuck::Garage, @14:49:19 Garaging FlushPuck in Collection Clamp, @14:49:33 Loading lyfil puck from tube 7 into Collection Clamp, @14:52:06 Storing lyfil puck in Collection Clamp to tube 6, @14:53:26 Loading FlushPuck into Processing Clamp from FlushPuck::Garage, @14:54:46 Garaging FlushPuck in Processing Clamp, @14:54:59 Loading da puck from tube 7 into Processing Clamp, @14:55:20 Heating Puck.from 7, :da in Processing Clamp to 28C for 2:00:00, @14:58:30 recoverPSRreagent08, @15:05:44 addPMLReagent08, @15:06:25 Beginning samplePassC, @15:06:27 Loading FlushPuck into Collection Clamp from FlushPuck::Garage, @15:10:06 Garaging FlushPuck in Collection Clamp, @15:10:20 Whole Cell Archive sampling 1000ml, @15:10:20 Loading wcr puck from tube 7 into Collection Clamp, @15:10:37 collectSample 1000ml w/bubble point of 35psi, @15:10:37 Priming 7ml, @15:10:38 Calibrating pressure sensors, @15:11:34 Sampling 1000.0ml, @15:41:06 Filtering too Slowly, @16:37:24 Sampled 450.0ml, @16:37:24 Drying puck before fixing for 5:00, @16:38:49 Pumping wcr puck from tube 7 up to 35psi...] @16:46:09.34 -> Thread.resume @16:46:09.79 Thread::CannotResume in dave -- No checkpoints available @16:46:32.90 -> MainThread.resume @16:46:32.92
Resuming Thread (suspended since 16:38:49) # @16:46:32.96 Pumping wcr puck from tube 7 up to 35psi... @16:46:33.00 CBV.dial SSV @16:46:34.44 CBV.dial between CSV and waste2b @16:46:35.43 CSV.dial between air and CTV @16:46:35.77 CS.reconfigure :fast @16:46:35.84 CS.seek 5.715ml @16:46:50.69 CTV.dial CSV,avoiding: :air @16:46:50.71
CSV.dial CTV @16:46:51.20 CS.reconfigure :pressurize @16:46:51.28 CS.seek 0ml @16:47:06.52 CSV.dial :air @16:47:06.98 CS.reconfigure :fast @16:47:07.06 CS.seek 10ml @16:47:31.27 CSV.dial between air and CTV @16:47:31.60 CS.seek 5.873ml @16:47:41.85 CTV.dial CSV,avoiding: :air @16:47:41.87
CSV.dial CTV @16:47:42.25 CS.reconfigure :pressurize @16:47:42.32 CS.seek 0ml @16:47:57.84 CSV.dial :air @16:47:58.36 CS.reconfigure :fast @16:47:58.43 CS.seek 10ml @16:48:22.65 CSV.dial between air and CTV @16:48:23.11 CS.seek 5.182ml @16:48:34.75 CTV.dial CSV,avoiding: :air @16:48:34.77
CSV.dial CTV @16:48:35.14 CS.reconfigure :pressurize @16:48:35.21 CS.seek 0ml @16:48:49.09 CSV.dial :air @16:48:49.56 CS.reconfigure :fast @16:48:49.63 CS.seek 10ml @16:49:14.40 CSV.dial between air and CTV @16:49:14.74 CS.seek 5.268ml @16:49:26.28 CTV.dial CSV,avoiding: :air @16:49:26.31
CSV.dial CTV @16:49:26.67 CS.reconfigure :pressurize @16:49:26.75 CS.seek 0ml @16:49:40.84 CSV.dial :air @16:49:41.31 CS.reconfigure :default @16:49:41.38 CS.hold @16:49:41.52 ShallowSampler::Error in MAIN -- Puck.from 7, :wcr reached only 21.6 of 35psi after pumping in 40ml of gas @16:49:41.54 Waiting to Resume from Checkpoint "Pumping wcr puck from tube 7 up to 35psi..." @16:49:41.57 BadNews.email "Puck.from 7, :wcr reached only 21.6 of 35psi after pumping in 40ml of gas",Subject: "ShallowSampler::Error in MAIN" @16:49:46.09 upsync @16:50:03.24 -> MainThread.resume @16:50:03.26
Resuming Thread (suspended since 16:38:49) # @16:50:03.31 Pumping wcr puck from tube 7 up to 35psi... @16:50:03.35 CBV.dial SSV @16:50:04.80 CBV.dial between CSV and waste2b @16:50:05.79 CSV.dial between air and CTV @16:50:06.14 CS.reconfigure :fast @16:50:06.22 CS.seek 5.234ml @16:50:20.06 CTV.dial CSV,avoiding: :air @16:50:20.10
CSV.dial CTV @16:50:20.48 CS.reconfigure :pressurize @16:50:20.56 CS.seek 0ml @16:50:34.55 CSV.dial :air @16:50:35.04 CS.reconfigure :fast @16:50:35.12 CS.seek 10ml @16:50:59.27 CSV.dial between air and CTV @16:50:59.73 CS.seek 5.664ml @16:51:10.40 CTV.dial CSV,avoiding: :air @16:51:10.84
CSV.dial CTV @16:51:11.29 CS.reconfigure :pressurize @16:51:11.37 CS.seek 0ml @16:51:24.95 CBV.dial :waste2b @16:51:25.31 CS.reconfigure :default @16:51:25.38 CS.seek 0ml @16:51:27.62 delay 1:00 @16:52:27.64 CTV.dial :air,avoiding: :intake @16:52:29.04 Fix #1 of 2 (with RNAlater) for 2:30 @16:52:29.06 CSV.dial CSR @16:52:29.52 Pulling 2ml of CSR.RNAlater @16:52:29.53 CSR.select :RNAlater @16:52:29.69 CS.seek 2ml @16:52:35.61 delay 5 seconds @16:52:40.63 CSR.select :air @16:52:40.78 CS.reconfigure :fast @16:52:40.85 CS.seek 10ml @16:53:00.40 Pumping wcr puck from tube 7 up to 35psi... @16:53:00.42 CBV.dial SSV @16:53:01.70 CBV.dial between CSV and waste2b @16:53:02.68 CSV.dial between air and CTV @16:53:03.29 CS.seek 7.017ml @16:53:10.93 CTV.dial CSV,avoiding: :air @16:53:10.96
CSV.dial CTV @16:53:12.37 CS.reconfigure :pressurize @16:53:12.45 CS.seek 0ml @16:53:25.63 delay 2:30 @16:55:55.66 CBV.dial :waste2b @16:55:56.02 CS.reconfigure :default @16:55:56.09 CS.seek 0ml @16:56:02.36 delay 1:00 @16:57:02.39 CTV.dial :air,avoiding: :intake @16:57:03.71 Fix #2 of 2 (with RNAlater) for 2:30 @16:57:03.72 CSV.dial CSR @16:57:04.20 Pulling 2ml of CSR.RNAlater @16:57:04.21 CSR.select :RNAlater @16:57:04.37 CS.seek 2ml @16:57:10.58 delay 5 seconds @16:57:15.60 CSR.select :air @16:57:15.73 CS.reconfigure :fast @16:57:15.80 CS.seek 10ml @16:57:35.46 Pumping wcr puck from tube 7 up to 35psi... @16:57:35.47 CBV.dial SSV @16:57:37.20 CBV.dial between CSV and waste2b @16:57:38.17 CSV.dial between air and CTV @16:57:38.79 CS.seek 7.048ml @16:57:46.37 CTV.dial CSV,avoiding: :air @16:57:46.39
CSV.dial CTV @16:57:47.82 CS.reconfigure :pressurize @16:57:47.90 CS.seek 0ml @16:58:06.51 CSV.dial :air @16:58:06.97 CS.reconfigure :fast @16:58:07.05 CS.seek 10ml @16:58:31.64 CSV.dial between air and CTV @16:58:31.98 CS.seek 3.911ml @16:58:46.59 CTV.dial CSV,avoiding: :air @16:58:46.62
CSV.dial CTV @16:58:46.99 CS.reconfigure :pressurize @16:58:47.07 CS.seek 0ml @16:58:57.67 CSV.dial :air @16:58:58.16 CS.reconfigure :fast @16:58:58.24 CS.seek 10ml @16:59:22.51 CSV.dial between air and CTV @16:59:22.85 CS.seek 4.467ml @16:59:36.52 CTV.dial CSV,avoiding: :air @16:59:36.56
CSV.dial CTV @16:59:36.88 CS.reconfigure :pressurize @16:59:36.95 CS.seek 0ml @16:59:48.98 CSV.dial :air @16:59:49.46 CS.reconfigure :fast @16:59:49.54 CS.seek 10ml @17:00:13.82 CSV.dial between air and CTV @17:00:14.29 CS.seek 5.884ml @17:00:24.52 CTV.dial CSV,avoiding: :air @17:00:24.54
CSV.dial CTV @17:00:24.90 CS.reconfigure :pressurize @17:00:24.97 CS.seek 0ml @17:00:40.64 CSV.dial :air @17:00:41.11 CS.reconfigure :default @17:00:41.18 CS.hold @17:00:41.32 ShallowSampler::Error in MAIN -- Puck.from 7, :wcr reached only 28.1 of 35psi after pumping in 40ml of gas @17:00:41.34 Waiting to Resume from Checkpoint "Pumping wcr puck from tube 7 up to 35psi..." @17:00:41.37 BadNews.email "Puck.from 7, :wcr reached only 28.1 of 35psi after pumping in 40ml of gas",Subject: "ShallowSampler::Error in MAIN" @17:00:45.93 upsync @17:00:56.16 -> MainThread.resume @17:00:56.18
Resuming Thread (suspended since 16:57:35) # @17:00:56.24 Pumping wcr puck from tube 7 up to 35psi... @17:00:56.28 CBV.dial SSV @17:00:57.73 CBV.dial between CSV and waste2b @17:00:58.70 CSV.dial between air and CTV @17:00:59.05 CS.reconfigure :fast @17:00:59.13 CS.seek 5.564ml @17:01:13.90 CTV.dial CSV,avoiding: :air @17:01:13.92
CSV.dial CTV @17:01:14.27 CS.reconfigure :pressurize @17:01:14.34 CS.seek 0ml @17:01:29.17 CSV.dial :air @17:01:29.64 CS.reconfigure :fast @17:01:29.72 CS.seek 10ml @17:01:54.04 CSV.dial between air and CTV @17:01:54.38 CS.seek 7.029ml @17:02:01.99 CTV.dial CSV,avoiding: :air @17:02:02.01
CSV.dial CTV @17:02:02.36 CS.reconfigure :pressurize @17:02:02.44 CS.seek 0ml @17:02:21.45 CSV.dial :air @17:02:21.99 CS.reconfigure :fast @17:02:22.06 CS.seek 10ml @17:02:46.32 CSV.dial between air and CTV @17:02:46.66 CS.seek 6.952ml @17:02:54.43 CTV.dial CSV,avoiding: :air @17:02:54.45
CSV.dial CTV @17:02:54.78 CS.reconfigure :pressurize @17:02:54.86 CS.seek 0ml @17:03:13.26 CSV.dial :air @17:03:13.78 CS.reconfigure :fast @17:03:13.85 CS.seek 10ml @17:03:38.10 CSV.dial between air and CTV @17:03:38.44 CS.seek 6.982ml @17:03:46.08 CTV.dial CSV,avoiding: :air @17:03:46.10
CSV.dial CTV @17:03:46.44 CS.reconfigure :pressurize @17:03:46.51 CS.seek 0ml @17:04:04.95 CSV.dial :air @17:04:05.42 CS.reconfigure :default @17:04:05.49 CS.hold @17:04:05.73 ShallowSampler::Error in MAIN -- Puck.from 7, :wcr reached only 1.2 of 35psi after pumping in 40ml of gas @17:04:05.75 Waiting to Resume from Checkpoint "Pumping wcr puck from tube 7 up to 35psi..." @17:04:05.78 BadNews.email "Puck.from 7, :wcr reached only 1.2 of 35psi after pumping in 40ml of gas",Subject: "ShallowSampler::Error in MAIN" @17:04:10.14 upsync @17:04:56.43 -> CC Collection Clamp contains wcr puck from tube 7 @17:04:59.40 -> PC Processing Clamp open @17:05:02.02 -> Flush FlushPuck is in FlushPuck::Garage @17:05:06.59 -> Puck.count @17:05:06.68 SE.seek :down @17:05:06.77 SE.stop @17:05:06.89 SC.reconfigure :tight @17:05:06.96 SC.seek 1 @17:05:23.51 SC.configure :limp @17:05:23.81 Elbow.reconfigure :tight @17:05:23.88 Elbow.seek 1 @17:05:25.76 Forearm.reconfigure :tight @17:05:25.84 Forearm.seek 1 + 450 counts @17:05:26.89 SE.reconfigure :place @17:05:26.97 SE.seek :beamBreak @17:05:28.12 SE.configure :default @17:05:28.25 SE.seek :down @17:05:28.74 SE.stop @17:05:29.38 Forearm.reconfigure :loose @17:05:29.45 Forearm.seek :retract @17:05:30.43 SC.reconfigure :tight @17:05:30.50 SC.seek 2 @17:05:34.07 SC.configure :limp @17:05:34.26 Elbow.seek 2 @17:05:34.66 Forearm.reconfigure :tight @17:05:34.74 Forearm.seek 2 + 450 counts @17:05:35.81 SE.reconfigure :place @17:05:35.89 SE.seek :beamBreak @17:05:37.08 SE.configure :default @17:05:37.21 SE.seek :down @17:05:37.65 SE.stop @17:05:38.34 Forearm.reconfigure :loose @17:05:38.41 Forearm.seek :retract @17:05:39.39 SC.reconfigure :tight @17:05:39.46 SC.seek 3 @17:05:43.04 SC.configure :limp @17:05:43.22 Elbow.seek 3 @17:05:43.59 Forearm.reconfigure :tight @17:05:43.66 Forearm.seek 3 + 450 counts @17:05:44.75 SE.reconfigure :place @17:05:44.83 SE.seek :beamBreak @17:05:45.95 SE.configure :default @17:05:46.08 SE.seek :down @17:05:46.53 SE.stop @17:05:47.21 Forearm.reconfigure :loose @17:05:47.29 Forearm.seek :retract @17:05:48.24 SC.reconfigure :tight @17:05:48.31 SC.seek 4 @17:05:51.89 SC.configure :limp @17:05:52.07 Elbow.seek 4 @17:05:52.57 Forearm.reconfigure :tight @17:05:52.64 Forearm.seek 4 + 450 counts @17:05:53.70 SE.reconfigure :place @17:05:53.79 SE.seek :beamBreak @17:05:54.95 SE.configure :default @17:05:55.09 SE.seek :down @17:05:55.54 SE.stop @17:05:56.22 Forearm.reconfigure :loose @17:05:56.29 Forearm.seek :retract @17:05:57.28 SC.reconfigure :tight @17:05:57.35 SC.seek 5 @17:06:00.92 SC.configure :limp @17:06:01.10 Elbow.seek 5 @17:06:01.55 Forearm.reconfigure :tight @17:06:01.63 Forearm.seek 5 + 450 counts @17:06:02.68 SE.reconfigure :place @17:06:02.76 SE.seek :beamBreak @17:06:03.92 SE.configure :default @17:06:04.05 SE.seek :down @17:06:04.51 SE.stop @17:06:05.19 Forearm.reconfigure :loose @17:06:05.27 Forearm.seek :retract @17:06:06.25 SC.reconfigure :tight @17:06:06.33 SC.seek 6 @17:06:09.95 SC.configure :limp @17:06:10.13 Elbow.seek 6 @17:06:10.33 Forearm.reconfigure :tight @17:06:10.41 Forearm.seek 6 + 450 counts @17:06:11.42 SE.reconfigure :place @17:06:11.50 SE.seek :beamBreak @17:06:15.23 SE.configure :default @17:06:15.36 SE.seek :down @17:06:16.49 Forearm.reconfigure :loose @17:06:16.57 Forearm.seek :retract @17:06:17.93 SE.stop @17:06:17.99 SC.reconfigure :tight @17:06:18.07 SC.seek 7 @17:06:21.68 SC.configure :limp @17:06:21.87 Elbow.seek 7 @17:06:22.43 Forearm.reconfigure :tight @17:06:22.50 Forearm.seek 7 + 450 counts @17:06:23.56 SE.reconfigure :place @17:06:23.64 SE.seek :beamBreak @17:06:25.14 SE.configure :default @17:06:25.27 SE.seek :down @17:06:26.12 SE.stop @17:06:26.40 Forearm.reconfigure :loose @17:06:26.47 Forearm.seek :retract {1 => 33, 2 => 33, 3 => 33, 4 => 33, 5 => 33, 6 => 2, 7 => 28} @17:07:49.74 -> CC.unloadPuck 6, :WCR @17:07:49.83 Puck::Warning in dave -- Unloading Puck.from 7, :wcr to Storage Carousel @17:07:49.85 Storing WCR puck in Collection Clamp to tube 6 @17:07:50.05 SC.reconfigure :tight @17:07:50.13 SC.seek 6 @17:07:53.71 SC.configure :limp @17:07:53.89 Elbow.seek 6 @17:07:54.36 Forearm.reconfigure :tight @17:07:54.44 Forearm.seek 6 + 450 counts @17:07:55.51 SE.reconfigure :pick @17:07:55.59 SE.seek :beamBreak @17:07:58.81 SE.configure :default @17:07:58.99 Elbow.reconfigure :limp @17:07:59.06 Forearm.reconfigure :loose @17:07:59.13 Forearm.seek 6 @17:07:59.39 Elbow.hold @17:07:59.48 Elbow.reconfigure :tight @17:07:59.60 SE.seek 1.17 Pucks below Top Plate @17:08:00.07 Forearm.force -20 @17:08:00.13 Forearm.seek 6 + 285 counts @17:08:00.81 Forearm.reconfigure :tight @17:08:00.88 Forearm.seek 6 + 450 counts @17:08:01.08 SE.seek 2.17 Pucks below Top Plate @17:08:01.72 Forearm.reconfigure :loose @17:08:01.79 Forearm.seek :retract @17:08:02.75 Elbow.seek CC @17:08:04.43 Elbow.reconfigure :loose @17:08:04.55 Forearm.seek CC @17:08:05.53 Hand.close @17:08:06.22 CC.seek :open @17:08:10.98 Forearm.seek :retract @17:08:12.00 Elbow.reconfigure :tight @17:08:12.07 Elbow.seek 6 @17:08:13.84 Elbow.reconfigure :limp @17:08:13.92 Forearm.seek 6 @17:08:14.96 Elbow.hold @17:08:15.05 Elbow.reconfigure :tight @17:08:15.12 Hand.open @17:08:15.83 Forearm.force -20 @17:08:15.89 Forearm.seek 6 + 314 counts @17:08:16.19 Forearm.reconfigure :tight @17:08:16.26 Forearm.seek 6 + 450 counts @17:08:16.53 SE.seek :down @17:08:17.66 Forearm.reconfigure :loose @17:08:17.74 Forearm.seek :retract @17:08:19.10 SE.stop @17:09:00.78 -> CC Collection Clamp open @17:09:02.40 -> PC Processing Clamp open @17:09:04.74 -> Flush FlushPuck is in FlushPuck::Garage @17:09:10.02 -> Puck.count @17:09:10.10 SE.seek :down @17:09:10.19 SE.stop @17:09:10.30 SC.reconfigure :tight @17:09:10.37 SC.seek 1 @17:09:26.51 SC.configure :limp @17:09:26.69 Elbow.seek 1 @17:09:27.04 Forearm.reconfigure :tight @17:09:27.58 Forearm.seek 1 + 450 counts @17:09:28.62 SE.reconfigure :place @17:09:28.75 SE.seek :beamBreak @17:09:29.91 SE.configure :default @17:09:30.04 SE.seek :down @17:09:30.55 SE.stop @17:09:31.17 Forearm.reconfigure :loose @17:09:31.24 Forearm.seek :retract @17:09:32.20 SC.reconfigure :tight @17:09:32.28 SC.seek 2 @17:09:35.85 SC.configure :limp @17:09:36.03 Elbow.seek 2 @17:09:36.58 Forearm.reconfigure :tight @17:09:36.66 Forearm.seek 2 + 450 counts @17:09:37.67 SE.reconfigure :place @17:09:37.75 SE.seek :beamBreak @17:09:38.92 SE.configure :default @17:09:39.05 SE.seek :down @17:09:39.52 SE.stop @17:09:40.18 Forearm.reconfigure :loose @17:09:40.25 Forearm.seek :retract @17:09:41.22 SC.reconfigure :tight @17:09:41.29 SC.seek 3 @17:09:44.82 SC.configure :limp @17:09:45.00 Elbow.seek 3 @17:09:45.37 Forearm.reconfigure :tight @17:09:45.44 Forearm.seek 3 + 450 counts @17:09:46.49 SE.reconfigure :place @17:09:46.58 SE.seek :beamBreak @17:09:47.70 SE.configure :default @17:09:47.83 SE.seek :down @17:09:48.27 SE.stop @17:09:48.96 Forearm.reconfigure :loose @17:09:49.03 Forearm.seek :retract @17:09:50.00 SC.reconfigure :tight @17:09:50.07 SC.seek 4 @17:09:53.65 SC.configure :limp @17:09:54.29 Elbow.seek 4 @17:09:54.87 Forearm.reconfigure :tight @17:09:54.94 Forearm.seek 4 + 450 counts @17:09:56.06 SE.reconfigure :place @17:09:56.18 SE.seek :beamBreak @17:09:57.34 SE.configure :default @17:09:57.47 SE.seek :down @17:09:58.01 SE.stop @17:09:58.61 Forearm.reconfigure :loose @17:09:58.69 Forearm.seek :retract @17:09:59.70 SC.reconfigure :tight @17:09:59.78 SC.seek 5 @17:10:03.34 SC.configure :limp @17:10:03.52 Elbow.seek 5 @17:10:03.80 Forearm.reconfigure :tight @17:10:03.88 Forearm.seek 5 + 450 counts @17:10:05.01 SE.reconfigure :place @17:10:05.09 SE.seek :beamBreak @17:10:06.24 SE.configure :default @17:10:06.38 SE.seek :down @17:10:06.83 SE.stop @17:10:07.51 Forearm.reconfigure :loose @17:10:07.58 Forearm.seek :retract @17:10:08.56 SC.reconfigure :tight @17:10:08.63 SC.seek 6 @17:10:12.16 SC.configure :limp @17:10:12.35 Elbow.seek 6 @17:10:12.55 Forearm.reconfigure :tight @17:10:12.63 Forearm.seek 6 + 450 counts @17:10:13.69 SE.reconfigure :place @17:10:13.77 SE.seek :beamBreak @17:10:16.99 SE.configure :default @17:10:17.12 SE.seek :down @17:10:18.27 Forearm.reconfigure :loose @17:10:18.34 Forearm.seek :retract @17:10:19.62 SE.stop @17:10:19.78 SC.reconfigure :tight @17:10:19.86 SC.seek 7 @17:10:23.45 SC.configure :limp @17:10:23.63 Elbow.seek 7 @17:10:23.95 Forearm.reconfigure :tight @17:10:24.03 Forearm.seek 7 + 450 counts @17:10:25.10 SE.reconfigure :place @17:10:25.19 SE.seek :beamBreak @17:10:26.73 SE.configure :default @17:10:26.87 SE.seek :down @17:10:27.69 SE.stop @17:10:28.00 Forearm.reconfigure :loose @17:10:28.07 Forearm.seek :retract {1 => 33, 2 => 33, 3 => 33, 4 => 33, 5 => 33, 6 => 3, 7 => 28} @17:10:51.23 -> abort! 'mission complete' @17:10:51.24 CmdServer::Client::Error in dave -- Aborting Mission: mission complete @17:10:51.29 BadNews.email "mission complete",Subject: "DA_WCR_121523 ABORTED!" # @17:10:55.71 upsync @17:10:55.80 Session Ended + ESPwhich DA_WCR_121823.rb + fn=DA_WCR_121823.rb + IFS=: + set /home/esp/esp2/mission . + fullPath=/home/esp/esp2/mission/DA_WCR_121823.rb + [ -f /home/esp/esp2/mission/DA_WCR_121823.rb ] + realpath /home/esp/esp2/mission/DA_WCR_121823.rb + return 0 + scriptPath=/home/esp/esp2local/mission/DA_WCR_121823.rb + exec ruby /home/esp/esp2/lib/esp.rb /home/esp/esp2local/mission/DA_WCR_121823.rb @13:55:25.03UTC18-Dec-23
ESP2 v3.42 I2C Gateway v3.20 for core bus @13:55:27.70 Configuration for short puck jake 1/11/21 by brent@mbari.org /home/esp/esp2/type/shallow/jake/configure.rb @13:55:31.32 Polling Can every 10:00 @13:55:31.55 Cleanly exited at 02:24:06UTC18-Dec-23 @13:55:31.60 Loading /home/esp/esp2local/mission/DA_WCR_121823.rb mission @13:55:31.65 GoodNews.email "Starting DA_WCR_121823 w/pucks in tube 7\n{1 => 33,\n 2 => 33,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 3,\n 7 => 28}\nCTD data unavailable\nCan@13:55:31, 31% flow, 16.5C, 34% humidity, 15.1psia, 13.730V, 0.315A, 249.212Ah, 4.32W",Subject: "DA_WCR_121823 begun" @13:55:31.68
Preparing to sleep until + 00:00:05 @13:55:31.73 Asleep til + 00:00:05 @13:55:31.75 Continuing at 13:55 @13:55:32.03 Gate.power :core,:OFF @13:55:32.05 Gate.power :raw,:OFF @13:55:32.06 Shutdown @13:55:35.56 GoodNews.email "Then, in tube 7: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_121823.rb:14\n{1 => 33,\n 2 => 33,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 3,\n 7 => 28}\nCTD data unavailable\nCan@13:55:31, 31% flow, 16.4C, 35% humidity, 15.1psia, 13.725V, 0.309A, 249.212Ah, 4.23W",Subject: "Asleep until 13:55" @13:55:36.64
Powering core bus @13:55:36.90 upsync @13:55:40.61 upsync @13:55:42.26
Gate.power :raw,:ON @13:55:43.09 CS.configure :default @13:55:43.14 PS.configure :default @13:55:43.19 SS.configure :default @13:55:43.24 CC.configure :default @13:55:43.28 PC.configure :default @13:55:43.36 Forearm.configure :loose @13:55:43.40 Elbow.configure :loose @13:55:43.47 SC.configure :loose @13:55:43.53 SE.configure :default @13:55:43.57 CH.configure :default @13:55:43.61 PH.configure :default @13:55:43.65 All dwarf controllers running firmware version 3.85 @13:55:43.76 Valve.reset @13:55:43.98 Intake.select :close @13:55:44.04 Exhaust.select :close @13:55:44.08 CTV.dial :air @13:55:44.16 PSV.dial :air @13:55:44.29
Forearm.home.seek :retract @13:55:45.09 Hand.close @13:55:45.13
Elbow.reconfigure :loose @13:55:45.25 Elbow.jog 1500 @13:55:45.27 PS.home.seek 0ml @13:55:45.45 CSV.dial :waste1 @13:55:45.84
Elbow.reconfigure :loose @13:55:45.89 Elbow.home.seek :clear @13:55:46.19 SSV.dial :spit,avoiding: :exhaust @13:55:47.07 CS.reconfigure :fast @13:55:47.10 SS.home.seek 0ml @13:55:47.20 CS.home.seek 0ml @13:55:50.36
SE.home.seek :down @13:55:50.97 SC.reconfigure :tight @13:55:51.02 SC.home.seek :initial @13:55:53.54 PS.stop @13:56:19.32
SC.configure :limp @13:56:19.37 Forearm.seek :retract @13:56:19.44 Elbow.seek :hammer @13:56:20.47 Forearm.reconfigure :tight @13:56:20.52 Forearm.seek retracted @13:56:21.19 PC.home.seek :open @13:56:21.22
CC.home.seek :open @13:56:24.61 Phase [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] @13:56:24.63 Notice.email "At 13:56:24 began in tube 7: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_121823.rb:14\n{1 => 33,\n 2 => 33,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 3,\n 7 => 28}\nCTD data unavailable\nCan@13:56:24, 31% flow, 16.5C, 35% humidity, 15.1psia, 13.710V, 0.315A, 249.218Ah, 4.31W",Subject: "Started DA_WCR_121823 phase initialPurge; da [1000, 1000] #da and wsr are run simultanelously" @13:56:24.66
Starting initialPurge @13:56:24.68 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @13:56:24.75 Hand.open @13:56:25.46 Elbow.reconfigure :tight @13:56:25.51 Elbow.seek FlushPuck::Garage @13:56:27.45 Elbow.reconfigure :loose @13:56:27.56 Forearm.reconfigure :loose @13:56:27.62 Forearm.seek :garage @13:56:28.59 Hand.close @13:56:29.05 upsync @13:56:29.33
Forearm.seek :retract @13:56:30.31 Elbow.reconfigure :tight @13:56:30.36 Elbow.seek CC @13:56:33.36 Elbow.reconfigure :loose @13:56:33.47 Forearm.reconfigure :tight @13:56:33.52 Forearm.seek CC @13:56:34.80 CC.seek :closed @13:56:39.37 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.43 @13:56:39.44 Hand.open @13:56:40.14 Forearm.reconfigure :loose @13:56:40.19 Forearm.seek :retract @13:56:41.17 Flushing the Collection Valve::Manifold @13:56:41.30 CSV.dial CSR @13:56:42.59 Pulling 5ml of CSR.flush @13:56:42.60 CSR.select :flush @13:56:42.72 CS.reconfigure :default @13:56:43.18 CS.seek 5ml @13:56:56.81 delay 5 seconds @13:57:01.82 CSR.select :air @13:57:01.99 CS.reconfigure :fast @13:57:02.04 CS.seek 7.501ml @13:57:08.87 CSV.dial :waste @13:57:10.15 CS.reconfigure :default @13:57:10.20 CS.seek 0ml @13:57:30.21 Purging external Intake @13:57:30.34 CSV.dial :flush @13:57:30.80 CS.reconfigure :fast @13:57:30.85 CS.seek 10ml @13:57:55.52 delay 5 seconds @13:58:00.55 CSV.dial CBV @13:58:00.59
CBV.dial CSV @13:58:01.35 CTV.dial :intake @13:58:02.13 Intake.select :open @13:58:02.24 CS.seek 0ml @13:58:25.96 Intake.select :close @13:58:26.06 CTV.dial :air @13:58:26.85 Flushing upper manifold @13:58:26.98 CSV.dial :flush @13:58:27.84 CS.seek 5ml @13:58:40.52 delay 5 seconds @13:58:45.54 CSV.dial :air @13:58:46.07 CS.seek 7.001ml @13:58:51.59 CSV.dial CBV @13:58:51.61
CBV.dial CSV @13:58:52.66 CTV.dial :air @13:58:52.75 CS.seek 0ml @13:59:09.43 delay 7 seconds @13:59:16.46 CS.seek 3ml @13:59:24.52 CSV.dial :waste @13:59:24.98 CS.seek 0ml @13:59:32.88 CSV.dial :flush @13:59:33.45 CS.seek 3ml @13:59:41.52 delay 5 seconds @13:59:46.54 CSV.dial :air @13:59:47.19 CS.seek 5ml @13:59:52.77 CSV.dial :air @13:59:52.86 CS.seek 0ml @14:00:05.08 delay 7 seconds @14:00:12.11 CS.seek 3ml @14:00:20.33 CSV.dial :waste @14:00:21.06 CS.seek 0ml @14:00:28.71 Garaging FlushPuck in Collection Clamp @14:00:28.78 Elbow.reconfigure :tight @14:00:28.83 Elbow.seek CC @14:00:29.10 Elbow.reconfigure :loose @14:00:29.20 Forearm.seek CC @14:00:30.24 Hand.close @14:00:30.83 CC.seek :open @14:00:35.89 Forearm.seek :retract @14:00:36.93 Elbow.reconfigure :tight @14:00:36.98 Elbow.seek :garage @14:00:39.93 Elbow.reconfigure :loose @14:00:40.03 Forearm.reconfigure :tight @14:00:40.08 Forearm.seek :garage @14:00:41.12 Hand.open @14:00:41.76 Forearm.reconfigure :loose @14:00:41.81 Forearm.seek :retract @14:00:42.78 da sampling at most 1000ml, wcr at most 1000ml @14:00:42.82 Loading sh1 puck from tube 7 into Collection Clamp @14:00:42.83 DAfans.email "Started da in tube 7 at 14:00:42UTC18-Dec-23\n{1 => 33,\n 2 => 33,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 3,\n 7 => 28}\nCTD data unavailable\nCan@13:56:24, 31% flow, 16.5C, 35% humidity, 15.1psia, 13.710V, 0.315A, 249.218Ah, 4.31W",Subject: "da sampling at most 1000ml, wcr at most 1000ml" @14:00:43.11
SC.reconfigure :tight @14:00:43.18 SC.seek 7 @14:00:47.32 upsync @14:00:53.01
SC.configure :limp @14:00:53.25 Elbow.reconfigure :tight @14:00:53.30 Elbow.seek 7 @14:00:55.27 Forearm.reconfigure :tight @14:00:55.32 Forearm.seek 7 + 450 counts @14:00:56.50 SE.reconfigure :pick @14:00:56.56 SE.seek :beamBreak @14:00:57.85 SE.configure :default @14:00:58.03 Elbow.seek 7 @14:00:58.22 Forearm.seek 7 + 300 counts @14:00:58.37 Forearm.reconfigure :loose @14:00:58.42 Hand.close @14:00:59.09 SE.seek :down @14:00:59.67
Forearm.seek :retract @14:01:00.08 SE.stop @14:01:00.70
Elbow.seek CC @14:01:02.33 Elbow.reconfigure :loose @14:01:02.43 Forearm.reconfigure :tight @14:01:02.48 Forearm.seek CC @14:01:03.80 CC.seek :closed @14:01:08.31 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.31 @14:01:08.32 Hand.open @14:01:09.01 Forearm.reconfigure :loose @14:01:09.06 Forearm.seek :retract @14:01:10.01 collectSample 1000ml w/bubble point of 24psi @14:01:10.02 Priming 7ml @14:01:10.03 Intake.select :close @14:01:10.04 Exhaust.select :close @14:01:10.06 SS.hold @14:01:10.27 Calibrating pressure and temperature sensors @14:01:10.30 CTV.dial :air @14:01:10.38 SSV.dial :spit,avoiding: :exhaust @14:01:10.46 SS.reconfigure :fast @14:01:10.51 SS.seek 5ml @14:01:13.38 SSV.dial CBV @14:01:13.40
CBV.dial SSV @14:01:14.74 delay 3.8 seconds @14:01:18.67 Sensor limits: Intake max 99.0psia; Outlet max 92.7psia @14:01:18.68 SSV.dial :spit @14:01:19.99 SS.seek 0ml @14:01:22.84 SSV.dial CBV @14:01:24.17 CTV.dial :intake @14:01:24.98 SSV.dial CBV @14:01:25.01
CBV.dial SSV @14:01:25.08 Allowing 40s for 7.0ml of remaining 7.0ml @14:01:25.09 Intake.select :open @14:01:27.26 Intake @14.2psia, vacuum from 8.7 to 11.7psi @14:01:27.28 SS.reconfigure :inhaling 2.20ml/s @14:01:27.32 SS.seek 7ml @14:01:34.12 Stroke completed -- waiting for pressure to equalize ... @14:01:34.13 SS.reconfigure :exhale @14:01:34.18 SS.hold @14:01:41.32 Intake.select :close @14:01:41.48 SSV.dial :exhaust @14:01:42.22 Exhaust.select :open @14:01:42.33 SS.seek 0ml @14:01:46.55 Exhaust.select :close @14:01:46.65 Sampling 1000.0ml @14:01:46.66 Intake.select :close @14:01:46.67 Exhaust.select :close @14:01:46.69 SS.reconfigure :default @14:01:46.73 SS.hold @14:01:46.94 CTV.dial :intake @14:01:47.12 SSV.dial CBV @14:01:47.14
CBV.dial SSV @14:01:47.94 Allowing 130s for 25.0ml of remaining 1000.0ml @14:01:47.95 Intake.select :open @14:01:50.12 Intake @14.9psia, vacuum from 9.4 to 12.4psi @14:01:50.13 SS.reconfigure :inhaling 2.20ml/s @14:01:50.18 SS.seek 25ml @14:01:58.15 Vacuum too high -- waiting up to 40 seconds @14:01:58.16 SS.reconfigure :exhale @14:01:58.22 SS.hold @14:02:01.04 Intake @15.9psia, vacuum from 10.0 to 13.0psi @14:02:01.06 SS.reconfigure :inhaling 1.76ml/s @14:02:01.10 SS.seek 25ml @14:02:09.72 Stroke completed -- waiting for pressure to equalize ... @14:02:09.73 SS.reconfigure :exhale @14:02:09.78 SS.hold @14:02:32.69 Intake.select :close @14:02:32.84 SSV.dial :exhaust @14:02:33.61 Exhaust.select :open @14:02:33.72 SS.seek 0ml @14:02:42.73 Exhaust.select :close @14:02:42.84 SSV.dial CBV @14:02:42.86
CBV.dial SSV @14:02:43.64 Allowing 130s for 25.0ml of remaining 975.0ml @14:02:43.65 Intake.select :open @14:02:45.82 Intake @15.5psia, vacuum from 10.0 to 13.0psi @14:02:45.83 SS.reconfigure :inhaling 1.76ml/s @14:02:45.89 SS.seek 25ml @14:03:02.92 Stroke completed -- waiting for pressure to equalize ... @14:03:02.93 SS.reconfigure :exhale @14:03:02.98 SS.hold @14:03:30.02 Intake.select :close @14:03:30.18 SSV.dial :exhaust @14:03:30.92 Exhaust.select :open @14:03:31.04 SS.seek 0ml @14:03:39.87 Exhaust.select :close @14:03:39.98 SSV.dial CBV @14:03:40.00
CBV.dial SSV @14:03:40.79 Allowing 130s for 25.0ml of remaining 950.0ml @14:03:40.81 Intake.select :open @14:03:42.97 Intake @15.6psia, vacuum from 10.0 to 13.0psi @14:03:42.99 SS.reconfigure :inhaling 1.76ml/s @14:03:43.03 SS.seek 25ml @14:04:00.32 Stroke completed -- waiting for pressure to equalize ... @14:04:00.33 SS.reconfigure :exhale @14:04:00.38 SS.hold @14:04:27.98 Intake.select :close @14:04:28.13 SSV.dial :exhaust @14:04:28.89 Exhaust.select :open @14:04:29.00 SS.seek 0ml @14:04:37.79 Exhaust.select :close @14:04:37.90 SSV.dial CBV @14:04:37.92
CBV.dial SSV @14:04:38.68 Allowing 130s for 25.0ml of remaining 925.0ml @14:04:38.70 Intake.select :open @14:04:40.87 Intake @15.7psia, vacuum from 10.0 to 13.0psi @14:04:40.88 SS.reconfigure :inhaling 1.76ml/s @14:04:40.93 SS.seek 25ml @14:04:57.96 Vacuum too high -- waiting up to 40 seconds @14:04:57.97 SS.reconfigure :exhale @14:04:58.02 SS.hold @14:05:02.39 Intake @15.5psia, vacuum from 10.0 to 13.0psi @14:05:02.40 SS.reconfigure :inhaling 1.41ml/s @14:05:02.45 SS.seek 25ml @14:05:03.41 Stroke completed -- waiting for pressure to equalize ... @14:05:03.42 SS.reconfigure :exhale @14:05:03.47 SS.hold @14:05:23.86 Intake.select :close @14:05:24.01 SSV.dial :exhaust @14:05:24.77 Exhaust.select :open @14:05:24.88 SS.seek 0ml @14:05:33.57 Exhaust.select :close @14:05:33.68 SSV.dial CBV @14:05:33.70
CBV.dial SSV @14:05:34.48 Allowing 130s for 25.0ml of remaining 900.0ml @14:05:34.49 Intake.select :open @14:05:37.07 Intake @15.7psia, vacuum from 10.0 to 13.0psi @14:05:37.10 SS.reconfigure :inhaling 1.41ml/s @14:05:37.15 SS.seek 25ml @14:05:58.01 Stroke completed -- waiting for pressure to equalize ... @14:05:58.02 SS.reconfigure :exhale @14:05:58.07 SS.hold @14:06:18.47 Intake.select :close @14:06:18.63 SSV.dial :exhaust @14:06:19.40 Exhaust.select :open @14:06:19.51 SS.seek 0ml @14:06:28.17 Exhaust.select :close @14:06:28.27 SSV.dial CBV @14:06:28.30
CBV.dial SSV @14:06:29.07 Allowing 130s for 25.0ml of remaining 875.0ml @14:06:29.08 Intake.select :open @14:06:31.25 Intake @15.7psia, vacuum from 10.0 to 13.0psi @14:06:31.26 SS.reconfigure :inhaling 1.41ml/s @14:06:31.31 SS.seek 25ml @14:06:52.02 Stroke completed -- waiting for pressure to equalize ... @14:06:52.03 SS.reconfigure :exhale @14:06:52.08 SS.hold @14:07:08.81 Intake.select :close @14:07:08.96 SSV.dial :exhaust @14:07:09.73 Exhaust.select :open @14:07:09.85 SS.seek 0ml @14:07:18.57 Exhaust.select :close @14:07:18.68 SSV.dial CBV @14:07:18.70
CBV.dial SSV @14:07:19.51 Allowing 130s for 25.0ml of remaining 850.0ml @14:07:19.52 Intake.select :open @14:07:21.69 Intake @15.8psia, vacuum from 10.0 to 13.0psi @14:07:21.71 SS.reconfigure :inhaling 1.41ml/s @14:07:21.76 SS.seek 25ml @14:07:42.38 Stroke completed -- waiting for pressure to equalize ... @14:07:42.39 SS.reconfigure :exhale @14:07:42.44 SS.hold @14:08:00.87 Intake.select :close @14:08:01.03 SSV.dial :exhaust @14:08:01.79 Exhaust.select :open @14:08:01.91 SS.seek 0ml @14:08:10.85 Exhaust.select :close @14:08:10.96 SSV.dial CBV @14:08:10.98
CBV.dial SSV @14:08:11.77 Allowing 130s for 25.0ml of remaining 825.0ml @14:08:11.78 Intake.select :open @14:08:13.95 Intake @15.7psia, vacuum from 10.0 to 13.0psi @14:08:13.97 SS.reconfigure :inhaling 1.41ml/s @14:08:14.01 SS.seek 25ml @14:08:34.27 Stroke completed -- waiting for pressure to equalize ... @14:08:34.28 SS.reconfigure :exhale @14:08:34.33 SS.hold @14:08:52.69 Intake.select :close @14:08:52.85 SSV.dial :exhaust @14:08:53.60 Exhaust.select :open @14:08:53.72 SS.seek 0ml @14:09:02.48 Exhaust.select :close @14:09:02.58 SSV.dial CBV @14:09:02.60
CBV.dial SSV @14:09:03.40 Allowing 130s for 25.0ml of remaining 800.0ml @14:09:03.41 Intake.select :open @14:09:05.58 Intake @15.7psia, vacuum from 10.0 to 13.0psi @14:09:05.59 SS.reconfigure :inhaling 1.41ml/s @14:09:05.64 SS.seek 25ml @14:09:26.05 Stroke completed -- waiting for pressure to equalize ... @14:09:26.06 SS.reconfigure :exhale @14:09:26.11 SS.hold @14:09:42.86 Intake.select :close @14:09:43.01 SSV.dial :exhaust @14:09:43.76 Exhaust.select :open @14:09:43.87 SS.seek 0ml @14:09:52.76 Exhaust.select :close @14:09:52.86 SSV.dial CBV @14:09:52.88
CBV.dial SSV @14:09:53.68 Allowing 130s for 25.0ml of remaining 775.0ml @14:09:53.69 Intake.select :open @14:09:55.86 Intake @15.8psia, vacuum from 10.0 to 13.0psi @14:09:55.87 SS.reconfigure :inhaling 1.41ml/s @14:09:55.92 SS.seek 25ml @14:10:16.32 Stroke completed -- waiting for pressure to equalize ... @14:10:16.33 SS.reconfigure :exhale @14:10:16.38 SS.hold @14:10:32.59 Intake.select :close @14:10:32.74 SSV.dial :exhaust @14:10:33.51 Exhaust.select :open @14:10:34.05 SS.seek 0ml @14:10:42.79 Exhaust.select :close @14:10:42.89 SSV.dial CBV @14:10:42.92
CBV.dial SSV @14:10:43.69 Allowing 130s for 25.0ml of remaining 750.0ml @14:10:43.70 Intake.select :open @14:10:45.87 Intake @15.7psia, vacuum from 10.0 to 13.0psi @14:10:45.89 SS.reconfigure :inhaling 1.41ml/s @14:10:45.94 SS.seek 25ml @14:11:06.50 Stroke completed -- waiting for pressure to equalize ... @14:11:06.51 SS.reconfigure :exhale @14:11:06.56 SS.hold @14:11:21.74 Intake.select :close @14:11:21.89 SSV.dial :exhaust @14:11:22.65 Exhaust.select :open @14:11:22.76 SS.seek 0ml @14:11:31.49 Exhaust.select :close @14:11:31.60 SSV.dial CBV @14:11:31.62
CBV.dial SSV @14:11:32.40 Allowing 130s for 25.0ml of remaining 725.0ml @14:11:32.41 Intake.select :open @14:11:34.57 Intake @15.7psia, vacuum from 10.0 to 13.0psi @14:11:34.58 SS.reconfigure :inhaling 1.41ml/s @14:11:34.63 SS.seek 25ml @14:11:55.00 Stroke completed -- waiting for pressure to equalize ... @14:11:55.01 SS.reconfigure :exhale @14:11:55.06 SS.hold @14:12:09.74 Intake.select :close @14:12:09.89 SSV.dial :exhaust @14:12:10.64 Exhaust.select :open @14:12:10.76 SS.seek 0ml @14:12:19.38 Exhaust.select :close @14:12:19.49 SSV.dial CBV @14:12:19.51
CBV.dial SSV @14:12:20.28 Allowing 130s for 25.0ml of remaining 700.0ml @14:12:20.30 Intake.select :open @14:12:22.46 Intake @15.8psia, vacuum from 10.0 to 13.0psi @14:12:22.48 SS.reconfigure :inhaling 1.41ml/s @14:12:22.53 SS.seek 25ml @14:12:43.17 Stroke completed -- waiting for pressure to equalize ... @14:12:43.18 SS.reconfigure :exhale @14:12:43.23 SS.hold @14:12:58.05 Intake.select :close @14:12:58.21 SSV.dial :exhaust @14:12:58.97 Exhaust.select :open @14:12:59.09 SS.seek 0ml @14:13:07.80 Exhaust.select :close @14:13:07.91 SSV.dial CBV @14:13:07.93
CBV.dial SSV @14:13:08.72 Allowing 130s for 25.0ml of remaining 675.0ml @14:13:08.73 Intake.select :open @14:13:10.90 Intake @15.8psia, vacuum from 10.0 to 13.0psi @14:13:10.91 SS.reconfigure :inhaling 1.41ml/s @14:13:10.96 SS.seek 25ml @14:13:31.55 Stroke completed -- waiting for pressure to equalize ... @14:13:31.56 SS.reconfigure :exhale @14:13:31.60 SS.hold @14:13:45.90 Intake.select :close @14:13:46.05 SSV.dial :exhaust @14:13:46.80 Exhaust.select :open @14:13:46.91 SS.seek 0ml @14:13:55.64 Exhaust.select :close @14:13:55.75 SSV.dial CBV @14:13:55.77
CBV.dial SSV @14:13:56.56 Allowing 130s for 25.0ml of remaining 650.0ml @14:13:56.57 Intake.select :open @14:13:58.74 Intake @15.8psia, vacuum from 10.0 to 13.0psi @14:13:58.76 SS.reconfigure :inhaling 1.41ml/s @14:13:58.80 SS.seek 25ml @14:14:19.11 Stroke completed -- waiting for pressure to equalize ... @14:14:19.12 SS.reconfigure :exhale @14:14:19.17 SS.hold @14:14:35.38 Intake.select :close @14:14:35.90 SSV.dial :exhaust @14:14:36.67 Exhaust.select :open @14:14:36.79 SS.seek 0ml @14:14:45.50 Exhaust.select :close @14:14:45.61 SSV.dial CBV @14:14:45.63
CBV.dial SSV @14:14:46.42 Allowing 130s for 25.0ml of remaining 625.0ml @14:14:46.43 Intake.select :open @14:14:48.60 Intake @15.7psia, vacuum from 10.0 to 13.0psi @14:14:48.62 SS.reconfigure :inhaling 1.41ml/s @14:14:48.66 SS.seek 25ml @14:15:09.36 Stroke completed -- waiting for pressure to equalize ... @14:15:09.37 SS.reconfigure :exhale @14:15:09.42 SS.hold @14:15:23.71 Intake.select :close @14:15:23.86 SSV.dial :exhaust @14:15:24.63 Exhaust.select :open @14:15:24.74 SS.seek 0ml @14:15:33.41 Exhaust.select :close @14:15:33.52 SSV.dial CBV @14:15:33.56
CBV.dial SSV @14:15:34.30 Allowing 130s for 25.0ml of remaining 600.0ml @14:15:34.31 Intake.select :open @14:15:36.48 Intake @15.9psia, vacuum from 10.0 to 13.0psi @14:15:36.49 SS.reconfigure :inhaling 1.41ml/s @14:15:36.54 SS.seek 25ml @14:15:57.15 Stroke completed -- waiting for pressure to equalize ... @14:15:57.16 SS.reconfigure :exhale @14:15:57.64 SS.hold @14:16:13.79 Intake.select :close @14:16:13.95 SSV.dial :exhaust @14:16:14.70 Exhaust.select :open @14:16:14.82 SS.seek 0ml @14:16:23.55 Exhaust.select :close @14:16:23.66 SSV.dial CBV @14:16:23.68
CBV.dial SSV @14:16:24.47 Allowing 130s for 25.0ml of remaining 575.0ml @14:16:24.48 Intake.select :open @14:16:26.64 Intake @15.6psia, vacuum from 10.0 to 13.0psi @14:16:26.66 SS.reconfigure :inhaling 1.41ml/s @14:16:26.70 SS.seek 25ml @14:16:47.34 Stroke completed -- waiting for pressure to equalize ... @14:16:47.35 SS.reconfigure :exhale @14:16:47.40 SS.hold @14:17:03.77 Intake.select :close @14:17:03.92 SSV.dial :exhaust @14:17:04.69 Exhaust.select :open @14:17:04.80 SS.seek 0ml @14:17:13.50 Exhaust.select :close @14:17:13.60 SSV.dial CBV @14:17:13.62
CBV.dial SSV @14:17:14.44 Allowing 130s for 25.0ml of remaining 550.0ml @14:17:14.45 Intake.select :open @14:17:16.61 Intake @15.7psia, vacuum from 10.0 to 13.0psi @14:17:16.62 SS.reconfigure :inhaling 1.41ml/s @14:17:16.67 SS.seek 25ml @14:17:37.35 Stroke completed -- waiting for pressure to equalize ... @14:17:37.37 SS.reconfigure :exhale @14:17:37.41 SS.hold @14:17:53.63 Intake.select :close @14:17:53.78 SSV.dial :exhaust @14:17:54.54 Exhaust.select :open @14:17:54.66 SS.seek 0ml @14:18:03.31 Exhaust.select :close @14:18:03.42 SSV.dial CBV @14:18:03.44
CBV.dial SSV @14:18:04.23 Allowing 130s for 25.0ml of remaining 525.0ml @14:18:04.24 Intake.select :open @14:18:06.41 Intake @15.8psia, vacuum from 10.0 to 13.0psi @14:18:06.43 SS.reconfigure :inhaling 1.41ml/s @14:18:06.48 SS.seek 25ml @14:18:26.92 Stroke completed -- waiting for pressure to equalize ... @14:18:26.93 SS.reconfigure :exhale @14:18:26.98 SS.hold @14:18:43.66 Intake.select :close @14:18:43.81 SSV.dial :exhaust @14:18:44.57 Exhaust.select :open @14:18:44.69 SS.seek 0ml @14:18:53.46 Exhaust.select :close @14:18:53.57 SSV.dial CBV @14:18:53.59
CBV.dial SSV @14:18:54.37 Allowing 130s for 25.0ml of remaining 500.0ml @14:18:54.38 Intake.select :open @14:18:56.55 Intake @15.8psia, vacuum from 10.0 to 13.0psi @14:18:56.57 SS.reconfigure :inhaling 1.41ml/s @14:18:56.62 SS.seek 25ml @14:19:16.87 Stroke completed -- waiting for pressure to equalize ... @14:19:16.88 SS.reconfigure :exhale @14:19:16.93 SS.hold @14:19:35.21 Intake.select :close @14:19:35.36 SSV.dial :exhaust @14:19:36.10 Exhaust.select :open @14:19:36.22 SS.seek 0ml @14:19:45.07 Exhaust.select :close @14:19:45.18 SSV.dial CBV @14:19:45.21
CBV.dial SSV @14:19:45.96 Allowing 130s for 25.0ml of remaining 475.0ml @14:19:45.98 Intake.select :open @14:19:48.14 Intake @15.8psia, vacuum from 10.0 to 13.0psi @14:19:48.16 SS.reconfigure :inhaling 1.41ml/s @14:19:48.21 SS.seek 25ml @14:20:08.15 Stroke completed -- waiting for pressure to equalize ... @14:20:08.16 SS.reconfigure :exhale @14:20:08.21 SS.hold @14:20:28.97 Intake.select :close @14:20:29.13 SSV.dial :exhaust @14:20:29.89 Exhaust.select :open @14:20:30.00 SS.seek 0ml @14:20:38.87 Exhaust.select :close @14:20:38.98 SSV.dial CBV @14:20:39.00
CBV.dial SSV @14:20:39.79 Allowing 130s for 25.0ml of remaining 450.0ml @14:20:39.80 Intake.select :open @14:20:41.97 Intake @15.8psia, vacuum from 10.0 to 13.0psi @14:20:41.99 SS.reconfigure :inhaling 1.41ml/s @14:20:42.03 SS.seek 25ml @14:21:02.10 Stroke completed -- waiting for pressure to equalize ... @14:21:02.11 SS.reconfigure :exhale @14:21:02.16 SS.hold @14:21:22.56 Intake.select :close @14:21:22.72 SSV.dial :exhaust @14:21:23.49 Exhaust.select :open @14:21:24.02 SS.seek 0ml @14:21:32.82 Exhaust.select :close @14:21:32.93 SSV.dial CBV @14:21:32.96
CBV.dial SSV @14:21:33.74 Allowing 130s for 25.0ml of remaining 425.0ml @14:21:33.75 Intake.select :open @14:21:35.92 Intake @15.8psia, vacuum from 10.0 to 13.0psi @14:21:35.94 SS.reconfigure :inhaling 1.41ml/s @14:21:35.99 SS.seek 25ml @14:21:56.54 Stroke completed -- waiting for pressure to equalize ... @14:21:56.55 SS.reconfigure :exhale @14:21:56.60 SS.hold @14:22:17.01 Intake.select :close @14:22:17.17 SSV.dial :exhaust @14:22:17.93 Exhaust.select :open @14:22:18.05 SS.seek 0ml @14:22:27.02 Exhaust.select :close @14:22:27.13 SSV.dial CBV @14:22:27.15
CBV.dial SSV @14:22:28.30 Allowing 130s for 25.0ml of remaining 400.0ml @14:22:28.32 Intake.select :open @14:22:30.48 Intake @15.8psia, vacuum from 10.0 to 13.0psi @14:22:30.50 SS.reconfigure :inhaling 1.41ml/s @14:22:30.54 SS.seek 25ml @14:22:50.29 Stroke completed -- waiting for pressure to equalize ... @14:22:50.30 SS.reconfigure :exhale @14:22:50.34 SS.hold @14:23:14.75 Intake.select :close @14:23:14.90 SSV.dial :exhaust @14:23:15.66 Exhaust.select :open @14:23:15.78 SS.seek 0ml @14:23:24.54 Exhaust.select :close @14:23:24.64 SSV.dial CBV @14:23:24.66
CBV.dial SSV @14:23:25.53 Allowing 130s for 25.0ml of remaining 375.0ml @14:23:25.54 Intake.select :open @14:23:27.71 Intake @15.7psia, vacuum from 10.0 to 13.0psi @14:23:27.73 SS.reconfigure :inhaling 1.41ml/s @14:23:27.77 SS.seek 25ml @14:23:48.22 Stroke completed -- waiting for pressure to equalize ... @14:23:48.23 SS.reconfigure :exhale @14:23:48.28 SS.hold @14:24:12.80 Intake.select :close @14:24:12.95 SSV.dial :exhaust @14:24:13.71 Exhaust.select :open @14:24:13.82 SS.seek 0ml @14:24:22.58 Exhaust.select :close @14:24:22.70 SSV.dial CBV @14:24:22.74
CBV.dial SSV @14:24:23.47 Allowing 130s for 25.0ml of remaining 350.0ml @14:24:23.49 Intake.select :open @14:24:26.08 Intake @16.0psia, vacuum from 10.0 to 13.0psi @14:24:26.10 SS.reconfigure :inhaling 1.41ml/s @14:24:26.15 SS.seek 25ml @14:24:40.53 Vacuum too high -- waiting up to 40 seconds @14:24:40.54 SS.reconfigure :exhale @14:24:40.60 SS.hold @14:24:51.92 Intake @16.4psia, vacuum from 10.0 to 13.0psi @14:24:51.93 SS.reconfigure :inhaling 1.13ml/s @14:24:51.98 SS.seek 25ml @14:25:00.02 Stroke completed -- waiting for pressure to equalize ... @14:25:00.03 SS.reconfigure :exhale @14:25:00.08 SS.hold @14:25:16.49 Intake.select :close @14:25:16.64 SSV.dial :exhaust @14:25:17.40 Exhaust.select :open @14:25:17.51 SS.seek 0ml @14:25:26.21 Exhaust.select :close @14:25:26.31 SSV.dial CBV @14:25:26.33
CBV.dial SSV @14:25:27.14 Allowing 130s for 25.0ml of remaining 325.0ml @14:25:27.16 Intake.select :open @14:25:29.32 Intake @15.7psia, vacuum from 10.0 to 13.0psi @14:25:29.34 SS.reconfigure :inhaling 1.13ml/s @14:25:29.38 SS.seek 25ml @14:25:54.06 Stroke completed -- waiting for pressure to equalize ... @14:25:54.07 SS.reconfigure :exhale @14:25:54.12 SS.hold @14:26:20.71 Intake.select :close @14:26:20.87 SSV.dial :exhaust @14:26:21.64 Exhaust.select :open @14:26:21.75 SS.seek 0ml @14:26:30.77 Exhaust.select :close @14:26:30.88 SSV.dial CBV @14:26:30.90
CBV.dial SSV @14:26:31.69 Allowing 130s for 25.0ml of remaining 300.0ml @14:26:31.70 Intake.select :open @14:26:33.87 Intake @15.9psia, vacuum from 10.0 to 13.0psi @14:26:33.89 SS.reconfigure :inhaling 1.13ml/s @14:26:33.93 SS.seek 25ml @14:26:51.15 Vacuum too high -- waiting up to 40 seconds @14:26:51.16 SS.reconfigure :exhale @14:26:51.22 SS.hold @14:27:04.00 Intake @17.5psia, vacuum from 10.0 to 13.0psi @14:27:04.02 SS.reconfigure :inhaling 0.91ml/s @14:27:04.07 SS.seek 25ml @14:27:10.50 Vacuum too high -- waiting up to 40 seconds @14:27:10.51 SS.reconfigure :exhale @14:27:10.56 SS.hold @14:27:15.52 Intake @17.4psia, vacuum from 10.0 to 13.0psi @14:27:15.54 SS.reconfigure :inhaling 0.73ml/s @14:27:15.58 SS.seek 25ml @14:27:18.45 Stroke completed -- waiting for pressure to equalize ... @14:27:18.46 SS.reconfigure :exhale @14:27:18.51 SS.hold @14:27:29.71 Intake.select :close @14:27:29.87 SSV.dial :exhaust @14:27:30.64 Exhaust.select :open @14:27:30.76 SS.seek 0ml @14:27:39.47 Exhaust.select :close @14:27:39.58 SSV.dial CBV @14:27:39.60
CBV.dial SSV @14:27:40.39 Allowing 130s for 25.0ml of remaining 275.0ml @14:27:40.40 Intake.select :open @14:27:42.57 Intake @15.8psia, vacuum from 10.0 to 13.0psi @14:27:42.58 SS.reconfigure :inhaling 0.73ml/s @14:27:42.63 SS.seek 25ml @14:28:18.86 Stroke completed -- waiting for pressure to equalize ... @14:28:18.87 SS.reconfigure :exhale @14:28:18.92 SS.hold @14:28:41.79 Intake.select :close @14:28:41.95 SSV.dial :exhaust @14:28:42.70 Exhaust.select :open @14:28:42.82 SS.seek 0ml @14:28:51.59 Exhaust.select :close @14:28:51.70 SSV.dial CBV @14:28:51.74
CBV.dial SSV @14:28:52.48 Allowing 130s for 25.0ml of remaining 250.0ml @14:28:52.50 Intake.select :open @14:28:54.66 Intake @15.8psia, vacuum from 10.0 to 13.0psi @14:28:54.68 SS.reconfigure :inhaling 0.73ml/s @14:28:54.73 SS.seek 25ml @14:29:31.15 Stroke completed -- waiting for pressure to equalize ... @14:29:31.16 SS.reconfigure :exhale @14:29:31.21 SS.hold @14:29:55.77 Intake.select :close @14:29:55.92 SSV.dial :exhaust @14:29:56.69 Exhaust.select :open @14:29:56.80 SS.seek 0ml @14:30:05.79 Exhaust.select :close @14:30:05.91 SSV.dial CBV @14:30:05.93
CBV.dial SSV @14:30:06.72 Allowing 130s for 25.0ml of remaining 225.0ml @14:30:06.73 Intake.select :open @14:30:08.90 Intake @15.9psia, vacuum from 10.0 to 13.0psi @14:30:08.91 SS.reconfigure :inhaling 0.73ml/s @14:30:08.96 SS.seek 25ml @14:30:44.84 Stroke completed -- waiting for pressure to equalize ... @14:30:44.85 SS.reconfigure :exhale @14:30:44.90 SS.hold @14:31:11.34 Intake.select :close @14:31:11.49 SSV.dial :exhaust @14:31:12.27 Exhaust.select :open @14:31:12.39 SS.seek 0ml @14:31:21.15 Exhaust.select :close @14:31:21.26 SSV.dial CBV @14:31:21.29
CBV.dial SSV @14:31:22.06 Allowing 130s for 25.0ml of remaining 200.0ml @14:31:22.07 Intake.select :open @14:31:24.24 Intake @15.8psia, vacuum from 10.0 to 13.0psi @14:31:24.25 SS.reconfigure :inhaling 0.73ml/s @14:31:24.30 SS.seek 25ml @14:31:57.11 Vacuum too high -- waiting up to 40 seconds @14:31:57.13 SS.reconfigure :exhale @14:31:57.18 SS.hold @14:32:12.10 Intake @16.3psia, vacuum from 10.0 to 13.0psi @14:32:12.12 SS.reconfigure :inhaling 0.59ml/s @14:32:12.17 SS.seek 25ml @14:32:16.76 Stroke completed -- waiting for pressure to equalize ... @14:32:16.77 SS.reconfigure :exhale @14:32:16.82 SS.hold @14:32:31.20 Intake.select :close @14:32:31.35 SSV.dial :exhaust @14:32:32.12 Exhaust.select :open @14:32:32.24 SS.seek 0ml @14:32:41.03 Exhaust.select :close @14:32:41.13 SSV.dial CBV @14:32:41.15
CBV.dial SSV @14:32:41.95 Allowing 130s for 25.0ml of remaining 175.0ml @14:32:41.96 Intake.select :open @14:32:44.13 Intake @15.8psia, vacuum from 10.0 to 13.0psi @14:32:44.14 SS.reconfigure :inhaling 0.59ml/s @14:32:44.19 SS.seek 25ml @14:33:28.88 Stroke completed -- waiting for pressure to equalize ... @14:33:28.89 SS.reconfigure :exhale @14:33:28.94 SS.hold @14:33:57.98 Intake.select :close @14:33:58.14 SSV.dial :exhaust @14:33:58.89 Exhaust.select :open @14:33:59.00 SS.seek 0ml @14:34:07.84 Exhaust.select :close @14:34:07.94 SSV.dial CBV @14:34:07.96
CBV.dial SSV @14:34:08.76 Allowing 130s for 25.0ml of remaining 150.0ml @14:34:08.77 Intake.select :open @14:34:10.94 Intake @15.7psia, vacuum from 10.0 to 13.0psi @14:34:10.95 SS.reconfigure :inhaling 0.59ml/s @14:34:11.00 SS.seek 25ml @14:34:55.91 Stroke completed -- waiting for pressure to equalize ... @14:34:55.92 SS.reconfigure :exhale @14:34:55.97 SS.hold @14:35:24.83 Intake.select :close @14:35:24.98 SSV.dial :exhaust @14:35:25.74 Exhaust.select :open @14:35:25.86 SS.seek 0ml @14:35:34.69 Exhaust.select :close @14:35:34.80 SSV.dial CBV @14:35:34.84
CBV.dial SSV @14:35:35.59 Allowing 130s for 25.0ml of remaining 125.0ml @14:35:35.60 Intake.select :open @14:35:37.76 Intake @15.8psia, vacuum from 10.0 to 13.0psi @14:35:37.77 SS.reconfigure :inhaling 0.59ml/s @14:35:37.82 SS.seek 25ml @14:36:17.39 Vacuum too high -- waiting up to 40 seconds @14:36:17.40 SS.reconfigure :exhale @14:36:17.89 SS.hold @14:36:36.21 Intake @17.5psia, vacuum from 10.0 to 13.0psi @14:36:36.23 SS.reconfigure :inhaling 0.47ml/s @14:36:36.28 SS.seek 25ml @14:36:43.35 Stroke completed -- waiting for pressure to equalize ... @14:36:43.36 SS.reconfigure :exhale @14:36:43.41 SS.hold @14:37:00.14 Intake.select :close @14:37:00.29 SSV.dial :exhaust @14:37:01.05 Exhaust.select :open @14:37:01.17 SS.seek 0ml @14:37:09.93 Exhaust.select :close @14:37:10.03 SSV.dial CBV @14:37:10.05
CBV.dial SSV @14:37:10.83 Allowing 130s for 25.0ml of remaining 100.0ml @14:37:10.85 Intake.select :open @14:37:13.02 Intake @15.8psia, vacuum from 10.0 to 13.0psi @14:37:13.03 SS.reconfigure :inhaling 0.47ml/s @14:37:13.08 SS.seek 25ml @14:38:08.67 Stroke completed -- waiting for pressure to equalize ... @14:38:08.69 SS.reconfigure :exhale @14:38:08.73 SS.hold @14:38:39.46 Intake.select :close @14:38:39.62 SSV.dial :exhaust @14:38:40.38 Exhaust.select :open @14:38:40.49 SS.seek 0ml @14:38:49.33 Exhaust.select :close @14:38:49.44 SSV.dial CBV @14:38:49.46
CBV.dial SSV @14:38:50.27 Allowing 130s for 25.0ml of remaining 75.0ml @14:38:50.28 Intake.select :open @14:38:52.45 Intake @15.9psia, vacuum from 10.0 to 13.0psi @14:38:52.46 SS.reconfigure :inhaling 0.47ml/s @14:38:52.51 SS.seek 25ml @14:39:48.58 Stroke completed -- waiting for pressure to equalize ... @14:39:48.59 SS.reconfigure :exhale @14:39:48.64 SS.hold @14:40:21.33 Intake.select :close @14:40:21.48 SSV.dial :exhaust @14:40:22.25 Exhaust.select :open @14:40:22.36 SS.seek 0ml @14:40:31.17 Exhaust.select :close @14:40:31.28 SSV.dial CBV @14:40:31.31
CBV.dial SSV @14:40:32.06 Allowing 130s for 25.0ml of remaining 50.0ml @14:40:32.08 Intake.select :open @14:40:34.24 Intake @15.9psia, vacuum from 10.0 to 13.0psi @14:40:34.25 SS.reconfigure :inhaling 0.47ml/s @14:40:34.30 SS.seek 25ml @14:41:29.89 Stroke completed -- waiting for pressure to equalize ... @14:41:29.90 SS.reconfigure :exhale @14:41:29.95 SS.hold @14:42:09.19 Intake.select :close @14:42:09.35 SSV.dial :exhaust @14:42:10.10 Exhaust.select :open @14:42:10.22 SS.seek 0ml @14:42:19.28 Exhaust.select :close @14:42:19.39 SSV.dial CBV @14:42:19.41
CBV.dial SSV @14:42:20.19 Allowing 130s for 25.0ml of remaining 25.0ml @14:42:20.20 Intake.select :open @14:42:22.36 Intake @15.8psia, vacuum from 10.0 to 13.0psi @14:42:22.37 SS.reconfigure :inhaling 0.47ml/s @14:42:22.42 SS.seek 24.99ml @14:43:17.48 Stroke completed -- waiting for pressure to equalize ... @14:43:17.49 SS.reconfigure :exhale @14:43:17.54 SS.hold @14:43:58.14 SS.reconfigure :default @14:43:58.19 SS.hold @14:43:58.61 Intake.select :close @14:43:58.84 ShallowSampler::Clogged in MAIN -- Intake @16psia & Outlet @14psia won't equalize after 1000.0ml @14:43:58.85 SSV.dial :exhaust @14:43:59.61 Exhaust.select :open @14:43:59.72 SS.reconfigure :exhale @14:43:59.77 SS.seek 0ml @14:44:08.83 Exhaust.select :close @14:44:08.93 Sampled 1000.0ml @14:44:08.95 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) Intake.select :close @14:44:09.02 CSV.dial :waste @14:44:09.19 SSV.dial CBV,avoiding: :exhaust @14:44:09.21 CS.seek 0ml @14:44:09.24
CBV.dial SSV @14:44:09.41 CSV.dial :air @14:44:10.16 CS.seek 10ml @14:44:11.19
CTV.dial :air @14:44:11.97 delay 15 seconds @14:44:26.99 SSV.dial :spit,avoiding: :exhaust @14:44:28.30 CTV.dial CSV,avoiding: :intake @14:44:28.33
SS.seek 0ml @14:44:28.47 SSV.dial CBV,avoiding: :exhaust @14:44:34.73 SSV.dial between CBV and plug @14:44:35.21 CBV.dial between SSV and waste2 @14:44:35.59 CSV.dial between air and CTV @14:44:35.89 CS.seek 8.436ml @14:44:40.27 CTV.dial CSV,avoiding: :air @14:44:40.29
CSV.dial CTV @14:44:40.64 CS.reconfigure :pressurize @14:44:40.69 CS.seek 0ml @14:44:56.86 BubblePoint: Intake @24.1psia, Outlet @0.9psia @14:44:56.87 CBV.dial SSV @14:44:57.22 SS.reconfigure :default @14:44:57.27 SS.seek 10ml @14:45:02.83 delay 15 seconds @14:45:17.96 Evacuated: Intake @23.7psia, Outlet @23.7psia @14:45:17.97 SSV.dial :spit,avoiding: :exhaust @14:45:19.00 SS.reconfigure :exhale @14:45:19.05 SS.seek 0ml @14:45:24.03 SS.reconfigure :fast @14:45:24.08 SS.seek 5ml @14:45:26.99 SSV.dial CBV,avoiding: :exhaust @14:45:27.01
CBV.dial SSV @14:45:28.34 CTV.dial :air,avoiding: :intake @14:45:29.70 CSV.dial CTV @14:45:29.73
SS.seek 10ml @14:45:31.38 CSV.dial :air @14:45:31.81 CS.reconfigure :fast @14:45:31.86 CS.seek 0ml @14:45:32.83
SSV.dial :spit,avoiding: :exhaust @14:45:34.14 SS.reconfigure :exhale @14:45:34.21 SS.seek 0ml @14:45:37.98 CS.stop @14:45:39.15
SS.reconfigure :fast @14:45:39.20 SS.seek 5ml @14:45:42.00 SSV.dial CBV,avoiding: :exhaust @14:45:42.03
CBV.dial SSV @14:45:44.38 SSV.dial :spit,avoiding: :exhaust @14:45:45.71 SS.reconfigure :default @14:45:45.75 SS.seek 0ml @14:45:48.92 SS.stop @14:45:48.96 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @14:45:48.97 Intake.select :close @14:45:49.04 CSV.dial :waste @14:45:49.17 SSV.dial CBV,avoiding: :exhaust @14:45:49.19
CBV.dial SSV @14:45:49.81 CS.seek 0ml @14:45:49.88 CSV.dial :air @14:45:50.56
CTV.dial :air @14:45:50.63 CS.seek 10ml @14:45:50.73
delay 15 seconds @14:46:05.75 SSV.dial :spit,avoiding: :exhaust @14:46:07.07 CTV.dial CSV,avoiding: :intake @14:46:07.12
SS.reconfigure :exhale @14:46:07.19 SS.seek 0ml @14:46:07.62 SSV.dial CBV,avoiding: :exhaust @14:46:15.01 SSV.dial between CBV and plug @14:46:15.50 CBV.dial between SSV and waste2 @14:46:15.90 CSV.dial between air and CTV @14:46:16.21 CS.seek 7.619ml @14:46:22.49 CTV.dial CSV,avoiding: :air @14:46:22.53
CSV.dial CTV @14:46:22.89 CS.reconfigure :pressurize @14:46:22.95 CS.seek 0ml @14:46:39.17 BubblePoint: Intake @24.2psia, Outlet @3.2psia @14:46:39.18 CBV.dial SSV @14:46:39.52 SS.reconfigure :default @14:46:39.56 SS.seek 10ml @14:46:45.15 delay 15 seconds @14:47:00.70 Evacuated: Intake @23.8psia, Outlet @23.9psia @14:47:00.72 SSV.dial :spit,avoiding: :exhaust @14:47:01.77 SS.reconfigure :exhale @14:47:01.82 SS.seek 0ml @14:47:06.83 SS.reconfigure :fast @14:47:06.88 SS.seek 5ml @14:47:09.80 SSV.dial CBV,avoiding: :exhaust @14:47:09.82
CBV.dial SSV @14:47:11.17 CTV.dial :air,avoiding: :intake @14:47:12.48 CSV.dial CTV @14:47:12.53
SS.seek 10ml @14:47:13.70 CSV.dial :air @14:47:14.14 CS.reconfigure :fast @14:47:14.19 CS.seek 0ml @14:47:15.22
SSV.dial :spit,avoiding: :exhaust @14:47:16.53 SS.reconfigure :exhale @14:47:16.58 SS.seek 0ml @14:47:18.48 CS.stop @14:47:21.65
SS.reconfigure :fast @14:47:21.69 SS.seek 5ml @14:47:24.44 SSV.dial CBV,avoiding: :exhaust @14:47:24.46
CBV.dial SSV @14:47:26.80 SSV.dial :spit,avoiding: :exhaust @14:47:28.13 SS.reconfigure :default @14:47:28.17 SS.seek 0ml @14:47:31.37 SS.stop @14:47:31.41 Lysing: Adding 2.0ml AqMeOH @14:47:31.42 CBV.dial between CSV and waste1,avoiding: :DBV @14:47:32.93 CTV.dial CSV @14:47:34.26 CSV.dial CSR @14:47:35.05 Pulling 1ml of CSR.AqMeOH @14:47:35.06 CSR.select :AqMeOH @14:47:35.17 CS.reconfigure :default @14:47:35.22 CS.seek 1ml @14:47:38.85 delay 5 seconds @14:47:43.87 CSR.select :air @14:47:44.03 CS.seek 6ml @14:47:57.66 delay 2 seconds @14:47:59.68 CSV.dial :waste2 @14:48:00.12 CS.reconfigure :fast @14:48:00.17 CS.seek 0ml @14:48:14.51 CSV.dial CSR @14:48:14.96 Pulling 2ml of CSR.AqMeOH @14:48:14.97 CSR.select :AqMeOH @14:48:15.09 CS.reconfigure :default @14:48:15.13 CS.seek 2ml @14:48:21.29 delay 5 seconds @14:48:26.31 CSR.select :air @14:48:26.48 CS.seek 4.999ml @14:48:35.10 delay 2 seconds @14:48:37.12 CSV.dial CTV @14:48:37.98 CS.seek 1ml @14:48:49.34 Before lysing Collection Puck Heater at 13.2C/29.7psia @14:48:49.36 Heating Puck.from 7, :sh1 in Collection Clamp to 70C for 10:00 @14:48:49.38 CH.seek 70C,8:00 @14:51:02.17 CH.hold 10:00,70C @14:51:02.38 Begun lysing Collection Puck Heater at 68.1C/36.7psia @15:01:02.39 Ended lysing Collection Puck Heater at 70C/35.5psia @15:01:02.41 CS.seek 0ml @15:01:05.78 CTV.dial between spare and air,avoiding: :intake @15:01:06.81 CSV.dial :air @15:01:07.27 CS.seek 5ml @15:01:20.90 CSV.dial CTV @15:01:21.36 delay 2 seconds @15:01:23.37 CSV.dial :air @15:01:23.82 delay 2 seconds @15:01:25.84 CSV.dial CTV @15:01:26.30 delay 2 seconds @15:01:28.32 CSV.dial :air @15:01:28.76 delay 2 seconds @15:01:30.78 CS.seek 0ml @15:01:44.46 Puck @77.3psia after final lysis pressurization @15:01:44.47 Recovering Lysate into Collection syringe @15:01:44.48 CSV.dial CBV @15:01:45.50 CBV.dial CSV @15:01:45.87 CS.seek 5ml @15:01:59.43 delay 5 seconds @15:02:04.45 CTV.dial :air @15:02:04.99 CS.seek 7ml @15:02:10.87 delay 5 seconds @15:02:15.89 CSV.dial :air2 @15:02:16.33 CS.seek 10ml @15:02:24.81 CSV.dial between air and CTV,avoiding: CBV @15:02:25.97 Lysing Complete @15:02:26.04 Storing sh1 puck in Collection Clamp to tube 6 @15:02:26.22 SC.reconfigure :tight @15:02:26.27 SC.seek 6 @15:02:29.84 SC.configure :limp @15:02:30.48 Elbow.reconfigure :tight @15:02:30.54 Elbow.seek 6 @15:02:32.24 Forearm.reconfigure :tight @15:02:32.29 Forearm.seek 6 + 450 counts @15:02:33.34 SE.reconfigure :pick @15:02:33.39 SE.seek :beamBreak @15:02:36.32 SE.configure :default @15:02:36.49 Elbow.reconfigure :limp @15:02:36.54 Forearm.reconfigure :loose @15:02:36.58 Forearm.seek 6 @15:02:36.84 Elbow.hold @15:02:36.90 Elbow.reconfigure :tight @15:02:37.00 SE.seek 2.18 Pucks below Top Plate @15:02:37.89 Forearm.force -20 @15:02:37.93 Forearm.seek 6 + 303 counts @15:02:38.22 Forearm.reconfigure :tight @15:02:38.27 Forearm.seek 6 + 450 counts @15:02:38.47 SE.seek 3.18 Pucks below Top Plate @15:02:39.03 Forearm.reconfigure :loose @15:02:39.08 Forearm.seek :retract @15:02:40.01 Elbow.seek CC @15:02:41.70 Elbow.reconfigure :loose @15:02:41.80 Forearm.seek CC @15:02:42.74 Hand.close @15:02:43.40 CC.seek :open @15:02:48.17 Forearm.seek :retract @15:02:49.15 Elbow.reconfigure :tight @15:02:49.20 Elbow.seek 6 @15:02:50.97 Elbow.reconfigure :limp @15:02:51.39 Forearm.seek 6 @15:02:52.43 Elbow.hold @15:02:52.50 Elbow.reconfigure :tight @15:02:52.55 Hand.open @15:02:53.28 Forearm.force -20 @15:02:53.32 Forearm.seek 6 + 279 counts @15:02:53.60 Forearm.reconfigure :tight @15:02:53.65 Forearm.seek 6 + 450 counts @15:02:53.94 SE.seek :down @15:02:55.07
Forearm.reconfigure :loose @15:02:55.12 Forearm.seek :retract @15:02:56.48 SE.stop @15:02:56.53
Tranferring DA @15:02:56.54 Beginning samplePassC @15:02:56.55 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @15:02:56.74 Elbow.seek FlushPuck::Garage @15:02:58.61 Elbow.reconfigure :loose @15:02:58.71 Forearm.seek :garage @15:02:59.76 Hand.close @15:03:00.52 Forearm.seek :retract @15:03:01.53 Elbow.reconfigure :tight @15:03:01.58 Elbow.seek CC @15:03:04.49 Elbow.reconfigure :loose @15:03:04.60 Forearm.reconfigure :tight @15:03:04.65 Forearm.seek CC @15:03:05.87 CC.seek :closed @15:03:10.40 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.36 @15:03:10.47 Hand.open @15:03:11.09 Forearm.reconfigure :loose @15:03:11.13 Forearm.seek :retract @15:03:12.07 PSV.dial PSR @15:03:13.46 Pulling 1.5ml of PSR.flush @15:03:13.47 PSR.select :flush @15:03:13.59 PS.seek 1.5ml @15:03:29.75 delay 5 seconds @15:03:34.77 PSV.dial :waste1 @15:03:35.24 PS.seek 0ml @15:03:51.59 PSV.dial PSR @15:03:52.06 PSR.select :air @15:03:52.18 PS.seek 2.5ml @15:04:18.57 PRV.dial CBV,avoiding: :ab2 @15:04:18.59
CBV.dial PRV @15:04:20.34 PSV.dial :mixloop @15:04:21.38 CTV.dial :air,avoiding: :intake @15:04:21.60 PTV.dial :PRVmixing @15:04:22.93 PS.seek 0ml @15:04:49.34 PSV.dial :air @15:04:49.82 PS.seek 2.5ml @15:05:16.12 PSV.dial :mixloop @15:05:16.60 PS.seek 0ml @15:05:43.00 PSV.dial :air @15:05:43.47 PS.seek 2.5ml @15:06:09.81 PSV.dial :mixloop @15:06:10.28 PS.seek 0ml @15:06:36.59 Garaging FlushPuck in Collection Clamp @15:06:36.66 Elbow.reconfigure :tight @15:06:36.71 Elbow.seek CC @15:06:36.97 Elbow.reconfigure :loose @15:06:37.07 Forearm.seek CC @15:06:38.10 Hand.close @15:06:38.78 CC.seek :open @15:06:43.46 Forearm.seek :retract @15:06:44.45 Elbow.reconfigure :tight @15:06:44.50 Elbow.seek :garage @15:06:47.47 Elbow.reconfigure :loose @15:06:47.57 Forearm.reconfigure :tight @15:06:47.62 Forearm.seek :garage @15:06:48.70 Hand.open @15:06:49.33 Forearm.reconfigure :loose @15:06:49.38 Forearm.seek :retract @15:06:50.38 PSV.dial :mixloop @15:06:50.45 PTV.dial :PRVmixing @15:06:50.53 PRV.dial CBV @15:06:50.55
CBV.dial PRV @15:06:50.69 CTV.dial :air,avoiding: :intake @15:06:50.70 Loading lyfil puck from tube 7 into Collection Clamp @15:06:50.94 SC.reconfigure :tight @15:06:50.99 SC.seek 7 @15:06:54.55 SC.configure :limp @15:06:54.76 Elbow.reconfigure :tight @15:06:54.81 Elbow.seek 7 @15:06:56.76 Forearm.reconfigure :tight @15:06:56.81 Forearm.seek 7 + 450 counts @15:06:57.96 SE.reconfigure :pick @15:06:58.01 SE.seek :beamBreak @15:06:59.36 SE.configure :default @15:06:59.53 Elbow.seek 7 @15:07:00.00 Forearm.seek 7 + 300 counts @15:07:00.25 Forearm.reconfigure :loose @15:07:00.30 Hand.close @15:07:00.94 SE.seek :down @15:07:01.15
Forearm.seek :retract @15:07:01.94 SE.stop @15:07:02.10
Elbow.seek CC @15:07:03.81 Elbow.reconfigure :loose @15:07:03.92 Forearm.reconfigure :tight @15:07:03.96 Forearm.seek CC @15:07:05.20 CC.seek :closed @15:07:09.72 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.21 @15:07:09.73 Hand.open @15:07:10.47 Forearm.reconfigure :loose @15:07:10.52 Forearm.seek :retract @15:07:11.49 CTV.dial CSV,avoiding: :intake @15:07:12.82 CSV.dial CTV @15:07:13.26 PS.seek 2.5ml @15:07:39.74 CS.seek 4.5ml @15:07:54.58 delay 8 seconds @15:08:02.60 PSV.dial :air @15:08:03.07 delay 2 seconds @15:08:05.09 PSV.dial :waste2 @15:08:05.83 PS.seek 0ml @15:08:32.12 CBV.dial between PRV and waste1 @15:08:32.44 CSV.dial :air @15:08:32.89 CS.seek 10ml @15:08:47.83 CSV.dial CTV @15:08:48.29 CS.seek 0ml @15:09:14.92 CBV.dial :waste1 @15:09:15.24 delay 5 seconds @15:09:20.26 CTV.dial :air,avoiding: :intake @15:09:22.10 Storing lyfil puck in Collection Clamp to tube 6 @15:09:22.28 SC.reconfigure :tight @15:09:22.33 SC.seek 6 @15:09:25.89 SC.configure :limp @15:09:26.11 Elbow.reconfigure :tight @15:09:26.16 Elbow.seek 6 @15:09:27.92 Forearm.reconfigure :tight @15:09:27.97 Forearm.seek 6 + 450 counts @15:09:29.00 SE.reconfigure :pick @15:09:29.06 SE.seek :beamBreak @15:09:32.05 SE.configure :default @15:09:32.21 Elbow.reconfigure :limp @15:09:32.26 Forearm.reconfigure :loose @15:09:32.31 Forearm.seek 6 @15:09:32.50 Elbow.hold @15:09:32.57 Elbow.reconfigure :tight @15:09:32.67 SE.seek 3.17 Pucks below Top Plate @15:09:33.10 Forearm.force -20 @15:09:33.14 Forearm.seek 6 + 301 counts @15:09:33.42 Forearm.reconfigure :tight @15:09:33.47 Forearm.seek 6 + 450 counts @15:09:33.67 SE.seek 4.17 Pucks below Top Plate @15:09:34.18 Forearm.reconfigure :loose @15:09:34.23 Forearm.seek :retract @15:09:35.16 Elbow.seek CC @15:09:36.84 Elbow.reconfigure :loose @15:09:36.94 Forearm.seek CC @15:09:38.38 Hand.close @15:09:39.02 CC.seek :open @15:09:43.77 Forearm.seek :retract @15:09:44.76 Elbow.reconfigure :tight @15:09:44.81 Elbow.seek 6 @15:09:46.58 Elbow.reconfigure :limp @15:09:46.63 Forearm.seek 6 @15:09:47.60 Elbow.hold @15:09:47.66 Elbow.reconfigure :tight @15:09:47.71 Hand.open @15:09:48.39 Forearm.force -20 @15:09:48.43 Forearm.seek 6 + 306 counts @15:09:48.73 Forearm.reconfigure :tight @15:09:48.78 Forearm.seek 6 + 450 counts @15:09:49.02 SE.seek :down @15:09:50.16
Forearm.reconfigure :loose @15:09:50.21 Forearm.seek :retract @15:09:51.43 SE.stop @15:09:51.48
PSV.dial :air @15:09:52.23 PS.seek 2.5ml @15:10:18.62 delay 1 second @15:10:19.63 PSV.dial :mixloop @15:10:20.09 delay 1 second @15:10:21.10 PSV.dial :air @15:10:21.57 delay 1 second @15:10:22.59 PSV.dial :mixloop @15:10:23.06 delay 1 second @15:10:24.07 PSV.dial :air @15:10:24.54 delay 1 second @15:10:25.56 PS.seek 0ml @15:10:51.99 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @15:10:52.61 Elbow.seek FlushPuck::Garage @15:10:54.45 Elbow.reconfigure :loose @15:10:54.55 Forearm.seek :garage @15:10:55.58 Hand.close @15:10:56.26 Forearm.seek :retract @15:10:57.28 Elbow.reconfigure :tight @15:10:57.33 Elbow.seek PC @15:10:58.59 Elbow.reconfigure :loose @15:10:58.69 Forearm.reconfigure :tight @15:10:58.74 Forearm.seek PC @15:10:59.93 PC.seek :closed @15:11:04.56 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.65 @15:11:04.63 Hand.open @15:11:05.33 Forearm.reconfigure :loose @15:11:05.38 Forearm.seek :retract @15:11:06.36 PRV.dial :air @15:11:06.82 PSV.dial :mixloop @15:11:07.36 PS.seek 0.3ml @15:11:11.27 PSV.dial PSR @15:11:12.45 Pulling 0.4ml of PSR.air @15:11:12.46 PSR.select :air @15:11:12.48 PS.seek 0.7ml @15:11:17.56 delay 3 seconds @15:11:20.58 PSR.select :air @15:11:20.60 PS.seek 2.5ml @15:11:39.77 delay 3 seconds @15:11:42.79 PSV.dial :mixloop @15:11:43.82 PTV.dial :mixing @15:11:44.58 PBV.dial :waste2 @15:11:45.61 PS.seek 0ml @15:12:11.99 Garaging FlushPuck in Processing Clamp @15:12:12.07 Elbow.reconfigure :tight @15:12:12.11 Elbow.seek PC @15:12:12.24 Elbow.reconfigure :loose @15:12:12.35 Forearm.seek PC @15:12:13.33 Hand.close @15:12:13.90 PC.seek :open @15:12:18.61 Forearm.seek :retract @15:12:19.57 Elbow.reconfigure :tight @15:12:19.61 Elbow.seek :garage @15:12:20.98 Elbow.reconfigure :loose @15:12:21.08 Forearm.reconfigure :tight @15:12:21.13 Forearm.seek :garage @15:12:22.16 Hand.open @15:12:22.83 Forearm.reconfigure :loose @15:12:22.88 Forearm.seek :retract @15:12:23.89 Loading da puck from tube 7 into Processing Clamp @15:12:24.12 SC.reconfigure :tight @15:12:24.17 SC.seek 7 @15:12:27.68 SC.configure :limp @15:12:27.90 Elbow.reconfigure :tight @15:12:27.94 Elbow.seek 7 @15:12:29.82 Forearm.reconfigure :tight @15:12:29.87 Forearm.seek 7 + 450 counts @15:12:30.91 SE.reconfigure :pick @15:12:30.96 SE.seek :beamBreak @15:12:32.45 SE.configure :default @15:12:32.62 Elbow.seek 7 @15:12:33.24 Forearm.seek 7 + 300 counts @15:12:33.38 Forearm.reconfigure :loose @15:12:33.42 Hand.close @15:12:34.03 SE.seek :down @15:12:34.24
Forearm.seek :retract @15:12:35.10 SE.stop @15:12:35.19
Elbow.seek PC @15:12:37.73 Elbow.reconfigure :loose @15:12:37.83 Forearm.reconfigure :tight @15:12:37.88 Forearm.seek PC @15:12:39.15 PC.seek :closed @15:12:43.78 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.65 @15:12:43.79 Hand.open @15:12:44.45 Forearm.reconfigure :loose @15:12:44.50 Forearm.seek :retract @15:12:45.47 Heating Puck.from 7, :da in Processing Clamp to 28C for 2:00:00 @15:12:45.49 PH.seek 28C,5:00 @15:13:29.27 PH.hold 2:00:00,28C @15:13:29.32 delay 1:30 @15:14:59.36 PSR.select :air @15:14:59.38 PSV.dial PSR @15:15:00.48 Pulling 0.5ml of PSR.wash @15:15:00.50 PSR.select :wash @15:15:00.68 PS.seek 0.5ml @15:15:06.58 delay 5 seconds @15:15:11.60 PSR.select :air @15:15:11.72 PS.seek 2ml @15:15:27.86 PTV.dial :puck @15:15:28.61 PSV.dial PTV @15:15:29.50 PBV.dial :waste1 @15:15:30.90 PS.seek 1.5ml @15:15:36.86 delay 3 seconds @15:15:39.88 PBV.dial between PSV and waste1 @15:15:40.24 PS.seek 0ml @15:15:56.42 delay 5:00 @15:20:56.44 recoverPSRreagent08 @15:20:56.45 PTV.dial :puck @15:20:56.64 PBV.dial :waste1 @15:20:57.01 delay 30 seconds @15:21:27.04 PBV.dial between PSV and waste1 @15:21:27.42 PSR.select :air @15:21:27.43 PSV.dial PSR @15:21:28.19 PS.reconfigure :fast @15:21:28.24 PS.seek 2.5ml @15:21:52.04 PSV.dial PTV @15:21:52.81 delay 5 seconds @15:21:57.83 PSV.dial PSR @15:21:58.61 delay 5 seconds @15:22:03.63 PSV.dial PTV @15:22:04.39 delay 5 seconds @15:22:09.78 PSV.dial :air @15:22:10.61 PS.seek 0ml @15:22:33.76 PTV.dial :PRVmixing,via: PRV @15:22:35.12 PSV.dial :mixloop @15:22:35.59 PRV.dial :ab1 @15:22:36.34 PS.reconfigure :slow @15:22:36.39 PS.seek 0.15ml @15:22:39.95 delay 2 seconds @15:22:41.97 CBV.dial PRV @15:22:41.99
PRV.dial CBV @15:22:42.56 PS.seek 0.35ml @15:22:47.23 delay 2 seconds @15:22:49.25 CBV.dial between PRV and PSV @15:22:49.57 PRV.dial :air @15:22:50.03 PS.seek 0.9ml @15:23:01.73 PRV.dial :waste2 @15:23:02.48 PS.seek 0.5ml @15:23:11.10 addPMLReagent08 @15:23:11.11 PSV.dial :air @15:23:11.58 PS.reconfigure :default @15:23:11.62 PS.seek 2ml @15:23:27.69 PSV.dial :PML @15:23:28.16 PTV.dial :mixing @15:23:28.91 PBV.dial :waste1 @15:23:29.35 PS.seek 1.85ml @15:23:31.76 PBV.dial between PSV and waste1 @15:23:32.12 PS.seek 0ml @15:23:51.88 delay 2 seconds @15:23:53.90 Beginning samplePassC @15:23:53.92 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @15:23:54.10 Elbow.reconfigure :tight @15:23:54.15 Elbow.seek FlushPuck::Garage @15:23:55.52 Elbow.reconfigure :loose @15:23:55.62 Forearm.seek :garage @15:23:56.60 Hand.close @15:23:57.40 Forearm.seek :retract @15:23:58.37 Elbow.reconfigure :tight @15:23:58.42 Elbow.seek CC @15:24:01.24 Elbow.reconfigure :loose @15:24:01.78 Forearm.reconfigure :tight @15:24:01.83 Forearm.seek CC @15:24:03.05 CC.seek :closed @15:24:07.64 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.49 @15:24:07.70 Hand.open @15:24:08.33 Forearm.reconfigure :loose @15:24:08.37 Forearm.seek :retract @15:24:09.32 PSV.dial PSR @15:24:10.38 Pulling 1.5ml of PSR.flush @15:24:10.39 PSR.select :flush @15:24:10.52 PS.seek 1.5ml @15:24:26.69 delay 5 seconds @15:24:31.71 PSV.dial :waste1 @15:24:32.20 PS.seek 0ml @15:24:48.32 PSV.dial PSR @15:24:48.79 PSR.select :air @15:24:48.91 PS.seek 2.5ml @15:25:15.29 PRV.dial CBV,avoiding: :ab2 @15:25:15.32
CBV.dial PRV @15:25:16.94 PSV.dial :mixloop @15:25:17.99 CTV.dial :air,avoiding: :intake @15:25:18.07 PTV.dial :PRVmixing @15:25:18.84 PS.seek 0ml @15:25:45.25 PSV.dial :air @15:25:45.73 PS.seek 2.5ml @15:26:12.10 PSV.dial :mixloop @15:26:12.59 PS.seek 0ml @15:26:38.97 PSV.dial :air @15:26:39.45 PS.seek 2.5ml @15:27:05.75 PSV.dial :mixloop @15:27:06.68 PS.seek 0ml @15:27:33.01 Garaging FlushPuck in Collection Clamp @15:27:33.08 Elbow.reconfigure :tight @15:27:33.13 Elbow.seek CC @15:27:33.45 Elbow.reconfigure :loose @15:27:33.55 Forearm.seek CC @15:27:34.58 Hand.close @15:27:35.26 CC.seek :open @15:27:39.87 Forearm.seek :retract @15:27:40.87 Elbow.reconfigure :tight @15:27:40.92 Elbow.seek :garage @15:27:43.84 Elbow.reconfigure :loose @15:27:43.94 Forearm.reconfigure :tight @15:27:43.99 Forearm.seek :garage @15:27:45.08 Hand.open @15:27:45.82 Forearm.reconfigure :loose @15:27:45.86 Forearm.seek :retract @15:27:46.85 delayUntil "15:31:53.89" @15:27:46.88 Whole Cell Archive sampling 1000ml @15:27:46.90 Loading wcr puck from tube 7 into Collection Clamp @15:27:47.37 Elbow.reconfigure :tight @15:27:47.43 Elbow.seek 7 @15:27:49.36 Forearm.reconfigure :tight @15:27:49.42 Forearm.seek 7 + 450 counts @15:27:50.53 SE.reconfigure :pick @15:27:50.59 SE.seek :beamBreak @15:27:52.12 SE.configure :default @15:27:52.28 Elbow.seek 7 @15:27:52.92 Forearm.seek 7 + 300 counts @15:27:53.65 Forearm.reconfigure :loose @15:27:53.70 Hand.close @15:27:54.27 SE.seek :down @15:27:54.48 Forearm.seek :retract @15:27:55.45 Elbow.seek CC @15:27:55.53 SE.stop @15:27:57.08 Elbow.reconfigure :loose @15:27:57.19 Forearm.reconfigure :tight @15:27:57.24 Forearm.seek CC @15:27:58.44 CC.seek :closed @15:28:02.99 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.25 @15:28:03.00 Hand.open @15:28:03.69 Forearm.reconfigure :loose @15:28:03.74 Forearm.seek :retract @15:28:04.70 collectSample 1000ml w/bubble point of 35psi @15:28:04.71 Priming 7ml @15:28:04.72 Intake.select :close @15:28:04.73 Exhaust.select :close @15:28:04.75 SS.hold @15:28:04.96 Calibrating pressure sensors @15:28:04.97 CTV.dial :air @15:28:04.99 SSV.dial :spit,avoiding: :exhaust @15:28:05.06 SS.reconfigure :fast @15:28:05.12 SS.seek 5ml @15:28:07.79 SSV.dial CBV @15:28:07.81 CBV.dial SSV @15:28:09.17 delay 3.8 seconds @15:28:13.11 Sensor limits: Intake max 100.4psia; Outlet max 92.2psia @15:28:13.12 SSV.dial :spit @15:28:14.47 SS.seek 0ml @15:28:17.31 SSV.dial CBV @15:28:18.66 CTV.dial :intake @15:28:19.43 SSV.dial CBV @15:28:19.45 CBV.dial SSV @15:28:19.55 Allowing 40s for 7.0ml of remaining 7.0ml @15:28:19.56 Intake.select :open @15:28:21.73 Intake @14.2psia, vacuum from 8.7 to 11.7psi @15:28:21.75 SS.reconfigure :inhaling 2.20ml/s @15:28:21.80 SS.seek 7ml @15:28:28.43 Stroke completed -- waiting for pressure to equalize ... @15:28:28.44 SS.reconfigure :exhale @15:28:28.50 SS.hold @15:28:51.02 Intake.select :close @15:28:51.19 SSV.dial :exhaust @15:28:51.96 Exhaust.select :open @15:28:52.08 SS.seek 0ml @15:28:56.36 Exhaust.select :close @15:28:56.48 Sampling 1000.0ml @15:28:56.49 Intake.select :close @15:28:56.50 Exhaust.select :close @15:28:56.52 SS.reconfigure :default @15:28:56.56 SS.hold @15:28:56.77 CTV.dial :intake @15:28:57.01 SSV.dial CBV @15:28:57.05 CBV.dial SSV @15:28:57.79 Allowing 130s for 25.0ml of remaining 1000.0ml @15:28:57.81 Intake.select :open @15:28:59.97 Intake @15.1psia, vacuum from 9.6 to 12.6psi @15:28:59.99 SS.reconfigure :inhaling 2.20ml/s @15:29:00.04 SS.seek 25ml @15:29:07.74 Vacuum too high -- waiting up to 40 seconds @15:29:07.75 SS.reconfigure :exhale @15:29:07.81 SS.hold @15:29:16.76 Intake @17.0psia, vacuum from 10.0 to 13.0psi @15:29:16.78 SS.reconfigure :inhaling 1.76ml/s @15:29:16.83 SS.seek 25ml @15:29:20.99 Vacuum too high -- waiting up to 40 seconds @15:29:21.00 SS.reconfigure :exhale @15:29:21.06 SS.hold @15:29:26.17 SS.reconfigure :inhaling 1.41ml/s @15:29:26.23 SS.seek 25ml @15:29:31.49 Vacuum too high -- waiting up to 40 seconds @15:29:31.51 SS.reconfigure :exhale @15:29:31.57 SS.hold @15:29:36.79 Intake @15.7psia, vacuum from 10.0 to 13.0psi @15:29:36.81 SS.reconfigure :inhaling 1.13ml/s @15:29:36.85 SS.seek 25ml @15:29:38.66 Stroke completed -- waiting for pressure to equalize ... @15:29:38.68 SS.reconfigure :exhale @15:29:38.73 SS.hold @15:30:09.40 Intake.select :close @15:30:09.56 SSV.dial :exhaust @15:30:10.36 Exhaust.select :open @15:30:10.48 SS.seek 0ml @15:30:19.63 Exhaust.select :close @15:30:19.74 SSV.dial CBV @15:30:19.78 CBV.dial SSV @15:30:20.54 Allowing 130s for 25.0ml of remaining 975.0ml @15:30:20.56 Intake.select :open @15:30:22.73 Intake @15.6psia, vacuum from 10.0 to 13.0psi @15:30:22.75 SS.reconfigure :inhaling 1.13ml/s @15:30:22.80 SS.seek 25ml @15:30:47.13 Stroke completed -- waiting for pressure to equalize ... @15:30:47.14 SS.reconfigure :exhale @15:30:47.19 SS.hold @15:31:27.39 SS.reconfigure :default @15:31:27.44 SS.hold @15:31:27.51 Intake.select :close @15:31:27.67 ShallowSampler::Clogged in WCR -- Intake @16psia & Outlet @14psia won't equalize after 50.0ml @15:31:27.68 SSV.dial :exhaust @15:31:28.44 Exhaust.select :open @15:31:28.56 SS.reconfigure :exhale @15:31:28.61 SS.seek 0ml @15:31:37.54 Exhaust.select :close @15:31:38.09 Sampled 50.0ml @15:31:38.11 Drying puck before fixing for 5:00 @15:31:38.12 CBV.dial :waste2b @15:31:38.89 delay 1:00 @15:31:53.90 recoverPRVreagent08 @15:31:53.92 PTV.dial :mixing @15:31:54.69 PBV.dial :waste1 @15:31:55.06 delay 30 seconds @15:32:25.09 PBV.dial between PSV and waste1 @15:32:25.47 PSV.dial :air @15:32:25.94 PS.reconfigure :fast @15:32:25.99 PS.seek 2.5ml @15:32:38.92 CSV.dial :air @15:32:39.38 CS.reconfigure :fast @15:32:39.43 CS.seek 10ml @15:32:49.90 PSV.dial :mixloop @15:32:50.37 delay 2 seconds @15:32:52.39 PSV.dial :air @15:32:52.86 delay 2 seconds @15:32:54.88 PSV.dial :mixloop @15:32:55.34 delay 2 seconds @15:32:57.36 PSV.dial :air @15:32:57.83 PS.seek 0ml @15:33:04.16 Pumping wcr puck from tube 7 up to 35psi... @15:33:04.17 CBV.dial SSV @15:33:05.44 CBV.dial between CSV and waste2b @15:33:06.42 CSV.dial between air and CTV @15:33:06.74 CS.seek 7.231ml @15:33:13.97 CTV.dial CSV,avoiding: :air @15:33:13.99 CSV.dial CTV @15:33:14.85 CS.reconfigure :pressurize @15:33:14.90 CS.seek 0ml @15:33:21.44 washCycleDA #1 of 2 @15:33:21.45 PSR.select :air @15:33:21.47 PSV.dial PSR @15:33:22.81 Pulling 0.5ml of PSR.wash @15:33:22.82 PSR.select :wash @15:33:22.94 PS.reconfigure :default @15:33:22.99 PS.seek 0.5ml @15:33:28.97 delay 5 seconds @15:33:33.87 CSV.dial :air @15:33:33.98 PSR.select :air @15:33:34.10 PS.seek 2ml @15:33:34.33 CS.reconfigure :fast @15:33:34.38 CS.seek 10ml @15:33:50.34 PTV.dial :puck @15:33:51.09 PSV.dial PTV @15:33:51.84 PBV.dial :waste1 @15:33:52.28 PS.seek 1.5ml @15:33:58.15 delay 3 seconds @15:33:59.37 CSV.dial between air and CTV @15:33:59.69 CS.seek 4.771ml @15:34:01.17 PBV.dial between PSV and waste1 @15:34:01.55 PS.seek 0ml @15:34:12.47 CTV.dial CSV,avoiding: :air @15:34:12.50 CSV.dial CTV @15:34:13.02 CS.reconfigure :pressurize @15:34:13.07 CS.seek 0ml @15:34:17.90 delay 2:00 @15:34:25.87 CSV.dial :air @15:34:26.32 CS.reconfigure :fast @15:34:26.37 CS.seek 10ml @15:34:50.96 CSV.dial between air and CTV @15:34:51.29 CS.seek 5.036ml @15:35:03.54 CTV.dial CSV,avoiding: :air @15:35:03.56 CSV.dial CTV @15:35:03.91 CS.reconfigure :pressurize @15:35:03.96 CS.seek 0ml @15:35:17.43 CSV.dial :air @15:35:17.89 CS.reconfigure :fast @15:35:17.95 CS.seek 10ml @15:35:42.46 CSV.dial between air and CTV @15:35:42.79 CS.seek 5.081ml @15:35:54.75 CTV.dial CSV,avoiding: :air @15:35:54.77 CSV.dial CTV @15:35:55.25 CS.reconfigure :pressurize @15:35:55.30 CS.seek 0ml @15:36:08.81 CSV.dial :air @15:36:09.25 CS.reconfigure :default @15:36:09.30 CS.hold @15:36:09.43 ShallowSampler::Error in MAIN -- Puck.from 7, :wcr reached only 17.5 of 35psi after pumping in 40ml of gas @15:36:09.45 Waiting to Resume from Checkpoint "Pumping wcr puck from tube 7 up to 35psi..." @15:36:09.47 BadNews.email "Puck.from 7, :wcr reached only 17.5 of 35psi after pumping in 40ml of gas",Subject: "ShallowSampler::Error in MAIN" @15:36:14.47 upsync @15:36:17.93 recoverPSRreagent08 @15:36:17.95 PTV.dial :puck @15:36:18.03 PBV.dial :waste1 @15:36:18.40 delay 30 seconds @15:36:48.43 PBV.dial between PSV and waste1 @15:36:48.81 PSR.select :air @15:36:48.83 PSV.dial PSR @15:36:49.61 PS.reconfigure :fast @15:36:49.66 PS.seek 2.5ml @15:37:13.52 PSV.dial PTV @15:37:14.29 delay 5 seconds @15:37:19.31 PSV.dial PSR @15:37:20.07 delay 5 seconds @15:37:25.10 PSV.dial PTV @15:37:25.98 delay 5 seconds @15:37:31.00 PSV.dial :air @15:37:31.78 PS.seek 0ml @15:37:55.15 washCycleDA #2 of 2 @15:37:55.16 PSR.select :air @15:37:55.18 PSV.dial PSR @15:37:56.51 Pulling 0.5ml of PSR.wash @15:37:56.52 PSR.select :wash @15:37:56.63 PS.reconfigure :default @15:37:56.68 PS.seek 0.5ml @15:38:02.56 delay 5 seconds @15:38:07.58 PSR.select :air @15:38:07.70 PS.seek 2ml @15:38:23.80 PTV.dial :puck @15:38:23.82 PSV.dial PTV @15:38:24.70 PBV.dial :waste1 @15:38:25.13 PS.seek 1.5ml @15:38:31.02 delay 3 seconds @15:38:34.04 PBV.dial between PSV and waste1 @15:38:34.41 PS.seek 0ml @15:38:50.56 delay 2:00 @15:40:50.58 recoverPSRreagent08 @15:40:50.59 PTV.dial :puck @15:40:50.61 PBV.dial :waste1 @15:40:50.98 delay 30 seconds @15:41:21.00 PBV.dial between PSV and waste1 @15:41:21.38 PSR.select :air @15:41:21.40 PSV.dial PSR @15:41:22.15 PS.reconfigure :fast @15:41:22.20 PS.seek 2.5ml @15:41:46.11 PSV.dial PTV @15:41:46.88 delay 5 seconds @15:41:51.90 PSV.dial PSR @15:41:52.66 delay 5 seconds @15:41:57.68 PSV.dial PTV @15:41:58.46 delay 5 seconds @15:42:03.48 PSV.dial :air @15:42:04.25 PS.seek 0ml @15:42:27.71 getPMLReagent08 @15:42:27.72 PRV.dial :air @15:42:28.65 delay 5 seconds @15:42:33.67 PTV.dial :PRVmixing @15:42:35.00 delay 5 seconds @15:42:40.03 PRV.dial :ab2 @15:42:40.49 PTV.dial :PRVmixing @15:42:40.56 PSV.dial :mixloop @15:42:41.06 PS.reconfigure :default @15:42:41.10 PS.seek 0.3ml @15:42:45.06 delay 2 seconds @15:42:47.08 PRV.dial :air @15:42:47.61 PS.seek 0.55ml @15:42:50.99 addPMLReagent08 @15:42:51.00 PSV.dial :air @15:42:51.60 PS.seek 2ml @15:43:07.24 PSV.dial :PML @15:43:07.69 PTV.dial :mixing @15:43:08.46 PBV.dial :waste1 @15:43:08.89 PS.seek 1.85ml @15:43:11.20 PBV.dial between PSV and waste1 @15:43:11.59 PS.seek 0ml @15:43:31.30 delay 2 seconds @15:43:33.32 delay 8:00 @15:51:33.35 recoverPRVreagent08 @15:51:33.36 PTV.dial :mixing @15:51:33.43 PBV.dial :waste1 @15:51:33.81 delay 30 seconds @15:52:03.83 PBV.dial between PSV and waste1 @15:52:04.21 PSV.dial :air @15:52:04.81 PS.reconfigure :fast @15:52:04.86 PS.seek 2.5ml @15:52:28.76 PSV.dial :mixloop @15:52:29.21 delay 2 seconds @15:52:31.23 PSV.dial :air @15:52:31.70 delay 2 seconds @15:52:33.71 PSV.dial :mixloop @15:52:34.18 delay 2 seconds @15:52:36.20 PSV.dial :air @15:52:36.80 PS.seek 0ml @15:53:00.04 washCycleDA #1 of 3 @15:53:00.05 PSR.select :air @15:53:00.07 PSV.dial PSR @15:53:01.41 Pulling 0.5ml of PSR.wash @15:53:01.42 PSR.select :wash @15:53:01.54 PS.reconfigure :default @15:53:01.59 PS.seek 0.5ml @15:53:07.51 delay 5 seconds @15:53:12.53 PSR.select :air @15:53:12.65 PS.seek 2ml @15:53:28.88 PTV.dial :puck @15:53:29.63 PSV.dial PTV @15:53:30.40 PBV.dial :waste1 @15:53:30.83 PS.seek 1.5ml @15:53:36.82 delay 3 seconds @15:53:39.84 PBV.dial between PSV and waste1 @15:53:40.22 PS.seek 0ml @15:53:56.35 delay 2:00 @15:55:56.38 recoverPSRreagent08 @15:55:56.39 PTV.dial :puck @15:55:56.58 PBV.dial :waste1 @15:55:56.96 delay 30 seconds @15:56:27.41 PBV.dial between PSV and waste1 @15:56:27.80 PSR.select :air @15:56:27.82 PSV.dial PSR @15:56:28.59 PS.reconfigure :fast @15:56:28.64 PS.seek 2.5ml @15:56:52.53 PSV.dial PTV @15:56:53.30 delay 5 seconds @15:56:58.32 PSV.dial PSR @15:56:59.08 delay 5 seconds @15:57:04.10 PSV.dial PTV @15:57:04.88 delay 5 seconds @15:57:09.90 PSV.dial :air @15:57:10.77 PS.seek 0ml @15:57:34.07 washCycleDA #2 of 3 @15:57:34.08 PSR.select :air @15:57:34.10 PSV.dial PSR @15:57:35.44 Pulling 0.5ml of PSR.wash @15:57:35.45 PSR.select :wash @15:57:35.57 PS.reconfigure :default @15:57:35.62 PS.seek 0.5ml @15:57:41.63 delay 5 seconds @15:57:46.65 PSR.select :air @15:57:46.77 PS.seek 2ml @15:58:02.87 PTV.dial :puck @15:58:02.95 PSV.dial PTV @15:58:03.72 PBV.dial :waste1 @15:58:04.16 PS.seek 1.5ml @15:58:10.06 delay 3 seconds @15:58:13.08 PBV.dial between PSV and waste1 @15:58:13.46 PS.seek 0ml @15:58:29.69 delay 2:00 @16:00:29.72 recoverPSRreagent08 @16:00:29.73 PTV.dial :puck @16:00:29.80 PBV.dial :waste1 @16:00:30.17 delay 30 seconds @16:01:00.20 PBV.dial between PSV and waste1 @16:01:00.58 PSR.select :air @16:01:00.60 PSV.dial PSR @16:01:01.36 PS.reconfigure :fast @16:01:01.41 PS.seek 2.5ml @16:01:25.28 PSV.dial PTV @16:01:26.05 delay 5 seconds @16:01:31.07 PSV.dial PSR @16:01:31.94 delay 5 seconds @16:01:36.96 PSV.dial PTV @16:01:37.70 delay 5 seconds @16:01:42.72 PSV.dial :air @16:01:43.50 PS.seek 0ml @16:02:06.97 washCycleDA #3 of 3 @16:02:06.98 PSR.select :air @16:02:07.00 PSV.dial PSR @16:02:08.33 Pulling 0.5ml of PSR.wash @16:02:08.34 PSR.select :wash @16:02:08.45 PS.reconfigure :default @16:02:08.50 PS.seek 0.5ml @16:02:14.40 delay 5 seconds @16:02:19.42 PSR.select :air @16:02:19.54 PS.seek 2ml @16:02:35.71 PTV.dial :puck @16:02:35.79 PSV.dial PTV @16:02:36.54 PBV.dial :waste1 @16:02:36.98 PS.seek 1.5ml @16:02:43.37 delay 3 seconds @16:02:46.39 PBV.dial between PSV and waste1 @16:02:46.77 PS.seek 0ml @16:03:03.15 delay 2:00 @16:05:03.18 recoverPSRreagent08 @16:05:03.19 PTV.dial :puck @16:05:03.28 PBV.dial :waste1 @16:05:03.65 delay 30 seconds @16:05:33.68 PBV.dial between PSV and waste1 @16:05:34.06 PSR.select :air @16:05:34.08 PSV.dial PSR @16:05:34.84 PS.reconfigure :fast @16:05:34.89 PS.seek 2.5ml @16:05:58.80 PSV.dial PTV @16:05:59.57 delay 5 seconds @16:06:04.59 PSV.dial PSR @16:06:05.35 delay 5 seconds @16:06:10.38 PSV.dial PTV @16:06:11.26 delay 5 seconds @16:06:16.28 PSV.dial :air @16:06:17.17 PS.seek 0ml @16:06:40.40 Gate.power :camera,:ON @16:06:40.41 Mixing DA Substrates @16:06:40.42 PSR.select :air @16:06:40.44 PSV.dial PSR @16:06:41.87 Pulling 0.25ml of PSR.sub1 @16:06:41.89 PSR.select :sub1 @16:06:42.08 PS.reconfigure :default @16:06:42.19 PS.seek 0.25ml @16:06:45.82 delay 5 seconds @16:06:50.84 Pulling 0.25ml of PSR.sub2 @16:06:50.85 PSR.select :sub2 @16:06:50.97 PS.seek 0.5ml @16:06:54.37 delay 5 seconds @16:06:59.39 PSR.select :air @16:06:59.50 PS.seek 2ml @16:07:15.58 PTV.dial :puck @16:07:15.66 PSV.dial PTV @16:07:16.44 PBV.dial :waste1 @16:07:16.90 PS.seek 1.5ml @16:07:22.80 delay 3 seconds @16:07:25.82 PBV.dial between PSV and waste1 @16:07:26.22 PS.seek 0ml @16:07:42.40 delay 10 seconds @16:07:52.43 recoverPSRreagent08 @16:07:52.44 PTV.dial :puck @16:07:52.51 PBV.dial :waste1 @16:07:52.90 delay 30 seconds @16:08:22.93 PBV.dial between PSV and waste1 @16:08:23.30 PSR.select :air @16:08:23.32 PSV.dial PSR @16:08:24.11 PS.reconfigure :fast @16:08:24.16 PS.seek 2.5ml @16:08:48.33 PSV.dial PTV @16:08:49.12 delay 5 seconds @16:08:54.14 PSV.dial PSR @16:08:54.91 delay 5 seconds @16:08:59.93 PSV.dial PTV @16:09:00.71 delay 5 seconds @16:09:05.73 PSV.dial :air @16:09:06.51 PS.seek 0ml @16:09:30.49 Additional air clear @16:09:30.50 PSR.select :air @16:09:30.52 PSV.dial PSR @16:09:31.88 Pulling 2ml of PSR.air @16:09:31.89 PSR.select :air @16:09:31.91 PS.reconfigure :default @16:09:32.44 PS.seek 2ml @16:09:53.71 delay 5 seconds @16:09:58.73 PTV.dial :puck @16:09:58.82 PSV.dial PTV @16:09:59.58 PBV.dial :waste1 @16:10:00.02 PS.seek 1.5ml @16:10:05.89 delay 3 seconds @16:10:08.91 PBV.dial between PSV and waste1 @16:10:09.29 delay 1 second @16:10:10.31 PSV.dial :waste1 @16:10:11.39 PS.seek 0ml @16:10:27.54 Processing Puck Heater at 28.1C before imaging @16:10:27.55 PH.coast @16:10:27.67 Imaging da puck in Processing Clamp while storing it to tube 6 @16:10:27.85 SC.reconfigure :tight @16:10:27.90 SC.seek 6 @16:10:31.48 SC.configure :limp @16:10:31.70 Elbow.reconfigure :tight @16:10:31.75 Elbow.seek 6 @16:10:33.51 Forearm.reconfigure :tight @16:10:33.56 Forearm.seek 6 + 450 counts @16:10:34.59 SE.reconfigure :pick @16:10:34.65 SE.seek :beamBreak @16:10:37.60 SE.configure :default @16:10:37.76 Elbow.reconfigure :limp @16:10:37.81 Forearm.reconfigure :loose @16:10:37.85 Forearm.seek 6 @16:10:38.10 Elbow.hold @16:10:38.16 Elbow.reconfigure :tight @16:10:38.26 SE.seek 4.17 Pucks below Top Plate @16:10:38.72 Forearm.force -20 @16:10:38.76 Forearm.seek 6 + 300 counts @16:10:39.04 Forearm.reconfigure :tight @16:10:39.09 Forearm.seek 6 + 450 counts @16:10:39.29 SE.seek 5.17 Pucks below Top Plate @16:10:39.84 Forearm.reconfigure :loose @16:10:40.38 Forearm.seek :retract @16:10:41.32 Elbow.seek PC @16:10:43.83 Elbow.reconfigure :loose @16:10:43.94 Forearm.seek PC @16:10:44.92 Hand.close @16:10:45.60 PC.seek :open @16:10:50.38 Forearm.seek :retract @16:10:51.35 Elbow.reconfigure :tight @16:10:51.40 Elbow.seek 6 @16:10:54.04 Forearm.reconfigure :tight @16:10:54.09 Forearm.seek 6 + 450 counts @16:10:55.11 Hand.open @16:10:55.79 SE.seek 4.82 Pucks below Top Plate @16:10:56.10 SE.reconfigure :seal @16:10:56.15 SE.seek 3.85 Pucks below Top Plate @16:10:56.63 Lights, camera, ACTION! @16:10:56.67 PuckCamera: snap -bin=2,2 -auto /var/log/esp/da23dec1816h1000ml.tif @16:11:16.20 Waiting >20 seconds for da to relinquish FlushPuck @16:11:19.59 SX HX9/16 (USB:30): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 19.584 seconds /var/log/esp/da23dec1816h1000ml.tif: TIFF Upload Complete @16:11:19.62 PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da23dec1816h1000ml15s.tif @16:11:35.23 SX HX9/16 (USB:30): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15 seconds /var/log/esp/midres/da23dec1816h1000ml15s.tif: TIFF Upload Complete @16:11:35.25 PuckCamera: snap -bin=2,2 20.0 /var/log/esp/midres/da23dec1816h1000ml20s.tif @16:11:56.01 SX HX9/16 (USB:30): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 20 seconds /var/log/esp/midres/da23dec1816h1000ml20s.tif: TIFF Upload Complete @16:11:56.03 Gate.power :camera,:OFF @16:11:56.05 DAfans.email "19.584 sec auto-exposure",Subject: "da23dec1816h1000ml images!" @16:11:56.49 Elbow.seek 6 @16:11:56.98 Forearm.seek 6 + 450 counts @16:11:57.06 SE.reconfigure :gently @16:11:57.11 SE.seek 4.57 Pucks below Top Plate @16:11:57.79 Elbow.reconfigure :limp @16:11:57.84 Forearm.reconfigure :loose @16:11:57.91 Forearm.seek 6 @16:11:58.14 Elbow.hold @16:11:58.21 Elbow.reconfigure :tight @16:11:58.26 SE.reconfigure :default @16:11:58.31 SE.seek 4.75 Pucks below Top Plate @16:11:59.12 Forearm.force -20 @16:11:59.17 Forearm.seek 6 + 281 counts @16:11:59.45 Forearm.reconfigure :tight @16:11:59.61 Forearm.seek 6 + 450 counts @16:11:59.87 SE.seek :down @16:12:01.09 Forearm.reconfigure :loose @16:12:01.20 Forearm.seek :retract @16:12:02.26 SE.stop @16:12:02.47 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @16:12:02.88 Elbow.seek FlushPuck::Garage @16:12:04.85 Elbow.reconfigure :loose @16:12:05.11 Forearm.seek :garage @16:12:06.15 Hand.close @16:12:07.03 Forearm.seek :retract @16:12:08.05 Elbow.reconfigure :tight @16:12:08.15 Elbow.seek PC @16:12:09.54 Elbow.reconfigure :loose @16:12:09.75 Forearm.reconfigure :tight @16:12:09.90 Forearm.seek PC @16:12:11.54 PC.seek :closed @16:12:16.33 Processing Clamp onSpring/offSpring for 16/26 samples, on/off torque 1.65 @16:12:16.46 Hand.open @16:12:18.13 Forearm.reconfigure :loose @16:12:18.41 Forearm.seek :retract @16:12:19.41 PSR.select :air @16:12:19.45 PSV.dial PSR @16:12:19.98 Pulling 0.5ml of PSR.flush @16:12:20.00 PSR.select :flush @16:12:20.17 PS.seek 0.5ml @16:12:26.17 delay 5 seconds @16:12:29.60 upsync @16:12:31.20 PSR.select :air @16:12:31.33 PS.seek 2.5ml @16:12:52.61 delay 1 second @16:12:53.63 PRV.dial :waste2,avoiding: :ab2 @16:12:55.52 PTV.dial :PRVmixing @16:12:56.84 PSV.dial :mixloop @16:12:57.90 PS.seek :empty @16:13:24.23 PSR.select :air @16:13:24.25 PSV.dial PSR @16:13:25.31 Pulling 0.5ml of PSR.flush @16:13:25.32 PSR.select :flush @16:13:25.45 PS.seek 0.5ml @16:13:31.42 delay 5 seconds @16:13:36.44 PSR.select :air @16:13:36.56 PS.seek 2.5ml @16:13:57.80 delay 1 second @16:13:58.82 PBV.dial :waste1 @16:13:59.19 PTV.dial :mixing @16:13:59.97 PSV.dial :mixloop @16:14:01.03 PS.seek :empty @16:14:27.45 PSV.dial PSR @16:14:28.50 PSR.select :flush @16:14:28.62 PS.seek 1ml @16:14:39.70 PSR.select :air @16:14:39.82 PS.seek 2.5ml @16:14:55.95 PSV.dial PTV @16:14:56.72 PTV.dial :puck @16:14:57.47 PBV.dial :waste1 @16:14:57.65 PS.seek 0ml @16:15:24.01 PSV.dial PSR @16:15:24.78 PSR.select :flush @16:15:24.90 PS.seek 0.5ml @16:15:30.82 PSR.select :air @16:15:30.94 PS.seek 2.5ml @16:15:52.14 PSV.dial PTV @16:15:53.03 PBV.dial PSV @16:15:53.58 PS.seek 0ml @16:16:19.85 PSV.dial PBV @16:16:20.32 PS.seek 2.5ml @16:16:46.67 PSV.dial :waste1 @16:16:47.42 PS.seek 0ml @16:17:13.76 PSV.dial PSR @16:17:14.67 PSR.select :air @16:17:14.69 PS.seek 2.5ml @16:17:41.10 PSV.dial PTV @16:17:41.86 PBV.dial PSV @16:17:41.94 PS.seek 0ml @16:18:08.29 PSV.dial PBV @16:18:08.78 PS.seek 2.5ml @16:18:35.17 PSV.dial :waste1 @16:18:35.91 PS.seek 0ml @16:19:02.26 PSV.dial PSR @16:19:02.73 PSR.select :flush @16:19:02.85 PS.seek 1ml @16:19:13.85 PSR.select :air @16:19:13.97 PS.seek 2.5ml @16:19:30.20 PSV.dial PTV @16:19:30.95 PTV.dial :puck @16:19:31.02 PBV.dial :waste1 @16:19:31.59 PS.seek 0ml @16:19:57.94 PSV.dial PSR @16:19:58.71 PSR.select :flush @16:19:58.84 PS.seek 0.5ml @16:20:04.88 PSR.select :air @16:20:05.00 PS.seek 2.5ml @16:20:26.23 PSV.dial PTV @16:20:26.99 PBV.dial PSV @16:20:27.56 PS.seek 0ml @16:20:54.10 PSV.dial PBV @16:20:54.58 PS.seek 2.5ml @16:21:20.91 PSV.dial :waste1 @16:21:21.65 PS.seek 0ml @16:21:47.97 PSV.dial PSR @16:21:48.44 PSR.select :air @16:21:48.47 PS.seek 2.5ml @16:22:15.04 PSV.dial PTV @16:22:15.79 PBV.dial PSV @16:22:15.87 PS.seek 0ml @16:22:42.25 PSV.dial PBV @16:22:42.75 PS.seek 2.5ml @16:23:09.13 PSV.dial :waste1 @16:23:09.87 PS.seek 0ml @16:23:36.26 Garaging FlushPuck in Processing Clamp @16:23:36.33 Elbow.reconfigure :tight @16:23:36.38 Elbow.seek PC @16:23:36.95 Elbow.reconfigure :loose @16:23:37.05 Forearm.seek PC @16:23:38.04 Hand.close @16:23:38.72 PC.seek :open @16:23:43.44 Forearm.seek :retract @16:23:44.46 Elbow.reconfigure :tight @16:23:44.51 Elbow.seek :garage @16:23:45.86 Elbow.reconfigure :loose @16:23:45.96 Forearm.reconfigure :tight @16:23:46.01 Forearm.seek :garage @16:23:47.09 Hand.open @16:23:47.77 Forearm.reconfigure :loose @16:23:47.82 Forearm.seek :retract @17:26:36.92 Session Begun @17:26:40.06 -> CC Collection Clamp contains wcr puck from tube 7 @17:26:42.69 -> PC Processing Clamp open @17:27:42.77 -> Puck.count @17:27:42.86 SE.seek :down @17:27:42.94 SE.stop @17:27:43.05 SC.reconfigure :tight @17:27:43.12 SC.seek 1 @17:27:59.24 SC.configure :limp @17:27:59.48 Elbow.reconfigure :tight @17:27:59.55 Elbow.seek 1 @17:28:01.44 Forearm.reconfigure :tight @17:28:01.51 Forearm.seek 1 + 450 counts @17:28:02.55 SE.reconfigure :place @17:28:02.63 SE.seek :beamBreak @17:28:03.77 SE.configure :default @17:28:03.90 SE.seek :down @17:28:04.37 SE.stop @17:28:05.03 Forearm.reconfigure :loose @17:28:05.10 Forearm.seek :retract @17:28:06.07 SC.reconfigure :tight @17:28:06.14 SC.seek 2 @17:28:09.76 SC.configure :limp @17:28:09.93 Elbow.seek 2 @17:28:10.59 Forearm.reconfigure :tight @17:28:10.66 Forearm.seek 2 + 450 counts @17:28:11.72 SE.reconfigure :place @17:28:11.81 SE.seek :beamBreak @17:28:12.91 SE.configure :default @17:28:13.05 SE.seek :down @17:28:13.50 SE.stop @17:28:14.18 Forearm.reconfigure :loose @17:28:14.25 Forearm.seek :retract @17:28:15.23 SC.reconfigure :tight @17:28:15.30 SC.seek 3 @17:28:18.91 SC.configure :limp @17:28:19.08 Elbow.seek 3 @17:28:19.50 Forearm.reconfigure :tight @17:28:19.58 Forearm.seek 3 + 450 counts @17:28:20.65 SE.reconfigure :place @17:28:20.73 SE.seek :beamBreak @17:28:21.97 SE.configure :default @17:28:22.11 SE.seek :down @17:28:22.56 SE.stop @17:28:23.23 Forearm.reconfigure :loose @17:28:23.31 Forearm.seek :retract @17:28:24.32 SC.reconfigure :tight @17:28:24.39 SC.seek 4 @17:28:27.97 SC.configure :limp @17:28:28.15 Elbow.seek 4 @17:28:28.74 Forearm.reconfigure :tight @17:28:28.81 Forearm.seek 4 + 450 counts @17:28:29.92 SE.reconfigure :place @17:28:30.01 SE.seek :beamBreak @17:28:31.14 SE.configure :default @17:28:31.28 SE.seek :down @17:28:31.70 SE.stop @17:28:32.41 Forearm.reconfigure :loose @17:28:32.48 Forearm.seek :retract @17:28:33.95 SC.reconfigure :tight @17:28:34.02 SC.seek 5 @17:28:37.58 SC.configure :limp @17:28:37.76 Elbow.seek 5 @17:28:38.08 Forearm.reconfigure :tight @17:28:38.16 Forearm.seek 5 + 450 counts @17:28:39.18 SE.reconfigure :place @17:28:39.27 SE.seek :beamBreak @17:28:40.43 SE.configure :default @17:28:40.56 SE.seek :down @17:28:41.02 SE.stop @17:28:41.68 Forearm.reconfigure :loose @17:28:41.76 Forearm.seek :retract @17:28:42.73 SC.reconfigure :tight @17:28:42.80 SC.seek 6 @17:28:46.44 SC.configure :limp @17:28:46.62 Elbow.seek 6 @17:28:46.82 Forearm.reconfigure :tight @17:28:46.90 Forearm.seek 6 + 450 counts @17:28:47.94 SE.reconfigure :place @17:28:48.02 SE.seek :beamBreak @17:28:50.96 SE.configure :default @17:28:51.09 SE.seek :down @17:28:52.22 Forearm.reconfigure :loose @17:28:52.29 Forearm.seek :retract @17:28:53.37 SE.stop @17:28:53.44 SC.reconfigure :tight @17:28:53.51 SC.seek 7 @17:28:57.06 SC.configure :limp @17:28:57.25 Elbow.seek 7 @17:28:57.56 Forearm.reconfigure :tight @17:28:57.64 Forearm.seek 7 + 450 counts @17:28:58.67 SE.reconfigure :place @17:28:58.76 SE.seek :beamBreak @17:29:00.54 SE.configure :default @17:29:00.67 SE.seek :down @17:29:01.87 Forearm.reconfigure :loose @17:29:01.94 Forearm.seek :retract @17:29:02.15 SE.stop {1 => 33, 2 => 33, 3 => 33, 4 => 33, 5 => 33, 6 => 6, 7 => 24} @17:29:34.12 -> CC.unloadPuck 6, :WCR @17:29:34.21 Puck::Warning in dave -- Unloading Puck.from 7, :wcr to Storage Carousel @17:29:34.23 Storing WCR puck in Collection Clamp to tube 6 @17:29:34.43 SC.reconfigure :tight @17:29:34.50 SC.seek 6 @17:29:38.08 SC.configure :limp @17:29:38.26 Elbow.seek 6 @17:29:38.79 Forearm.reconfigure :tight @17:29:38.86 Forearm.seek 6 + 450 counts @17:29:39.91 SE.reconfigure :pick @17:29:39.99 SE.seek :beamBreak @17:29:42.85 SE.configure :default @17:29:43.03 Elbow.reconfigure :limp @17:29:43.10 Forearm.reconfigure :loose @17:29:43.17 Forearm.seek 6 @17:29:43.44 Elbow.hold @17:29:43.52 Elbow.reconfigure :tight @17:29:43.64 SE.seek 5.16 Pucks below Top Plate @17:29:44.21 Forearm.force -20 @17:29:44.26 Forearm.seek 6 + 284 counts @17:29:44.56 Forearm.reconfigure :tight @17:29:44.63 Forearm.seek 6 + 450 counts @17:29:44.84 SE.seek 6.16 Pucks below Top Plate @17:29:45.42 Forearm.reconfigure :loose @17:29:45.49 Forearm.seek :retract @17:29:46.44 Elbow.seek CC @17:29:48.09 Elbow.reconfigure :loose @17:29:48.21 Forearm.seek CC @17:29:49.21 Hand.close @17:29:49.79 CC.seek :open @17:29:54.55 Forearm.seek :retract @17:29:55.55 Elbow.reconfigure :tight @17:29:55.63 Elbow.seek 6 @17:29:57.42 Elbow.reconfigure :limp @17:29:57.88 Forearm.seek 6 @17:29:58.94 Elbow.hold @17:29:59.03 Elbow.reconfigure :tight @17:29:59.10 Hand.open @17:29:59.79 Forearm.force -20 @17:29:59.84 Forearm.seek 6 + 307 counts @17:30:00.14 Forearm.reconfigure :tight @17:30:00.22 Forearm.seek 6 + 450 counts @17:30:00.49 SE.seek :down @17:30:01.62 Forearm.reconfigure :loose @17:30:01.69 Forearm.seek :retract @17:30:02.83 SE.stop @17:30:06.30 -> CC Collection Clamp open @17:30:08.33 -> PC Processing Clamp open @17:30:19.42 -> Flush FlushPuck is in FlushPuck::Garage @17:30:22.89 -> Flush.CC @17:30:22.90 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @17:30:23.12 Elbow.seek FlushPuck::Garage @17:30:24.98 Elbow.reconfigure :loose @17:30:25.10 Forearm.seek :garage @17:30:26.07 Hand.close @17:30:26.78 Forearm.seek :retract @17:30:27.76 Elbow.reconfigure :tight @17:30:27.83 Elbow.seek CC @17:30:30.81 Elbow.reconfigure :loose @17:30:30.93 Forearm.reconfigure :tight @17:30:31.00 Forearm.seek CC @17:30:32.26 CC.seek :closed @17:30:36.82 Collection Clamp onSpring/offSpring for 14/27 samples, on/off torque 2.32 @17:30:36.91 Hand.open @17:30:37.59 Forearm.reconfigure :loose @17:30:37.66 Forearm.seek :retract FlushPuck::Garage @17:31:48.77 -> Flush.garage @17:31:48.79 Garaging FlushPuck in Collection Clamp @17:31:49.35 Elbow.reconfigure :tight @17:31:49.43 Elbow.seek CC @17:31:49.72 Elbow.reconfigure :loose @17:31:49.84 Forearm.seek CC @17:31:50.89 Hand.close @17:31:51.54 CC.seek :open @17:31:56.18 Forearm.seek :retract @17:31:57.16 Elbow.reconfigure :tight @17:31:57.23 Elbow.seek :garage @17:32:00.20 Elbow.reconfigure :loose @17:32:00.32 Forearm.reconfigure :tight @17:32:00.39 Forearm.seek :garage @17:32:01.46 Hand.open @17:32:02.09 Forearm.reconfigure :loose @17:32:02.16 Forearm.seek :retract FlushPuck::Garage @17:32:37.83 -> Flush FlushPuck is in FlushPuck::Garage @17:32:52.52 -> abort! 'mission complete' @17:32:52.54 CmdServer::Client::Error in dave -- Aborting Mission: mission complete @17:32:52.58 BadNews.email "mission complete",Subject: "DA_WCR_121823 ABORTED!" # @17:32:57.27 upsync @17:32:57.37 Session Ended + ESPwhich DA_WCR_121923.rb + fn=DA_WCR_121923.rb + IFS=: + set /home/esp/esp2/mission . + fullPath=/home/esp/esp2/mission/DA_WCR_121923.rb + [ -f /home/esp/esp2/mission/DA_WCR_121923.rb ] + realpath /home/esp/esp2/mission/DA_WCR_121923.rb + return 0 + scriptPath=/home/esp/esp2local/mission/DA_WCR_121923.rb + exec ruby /home/esp/esp2/lib/esp.rb /home/esp/esp2local/mission/DA_WCR_121923.rb @16:29:44.30UTC19-Dec-23
ESP2 v3.42 I2C Gateway v3.20 for core bus @16:29:46.95 Configuration for short puck jake 1/11/21 by brent@mbari.org /home/esp/esp2/type/shallow/jake/configure.rb @16:29:50.51 Polling Can every 10:00 @16:29:50.75 Cleanly exited at 16:29:20UTC19-Dec-23 @16:29:50.79 Loading /home/esp/esp2local/mission/DA_WCR_121923.rb mission @16:29:50.85 GoodNews.email "Starting DA_WCR_121923 w/pucks in tube 7\n{1 => 33,\n 2 => 33,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 7,\n 7 => 24}\nCTD data unavailable\nCan@16:29:50, 31% flow, 16.2C, 36% humidity, 15.1psia, 13.637V, 0.309A, 257.276Ah, 4.21W",Subject: "DA_WCR_121923 begun" @16:29:50.89
Preparing to sleep until + 00:00:05 @16:29:50.92 Asleep til + 00:00:05 @16:29:50.94 Continuing at 16:29 @16:29:51.22 Gate.power :core,:OFF @16:29:51.24 Gate.power :raw,:OFF Shutdown @16:29:54.72 GoodNews.email "Then, in tube 7: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_121923.rb:14\n{1 => 33,\n 2 => 33,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 7,\n 7 => 24}\nCTD data unavailable\nCan@16:29:50, 31% flow, 16.2C, 36% humidity, 15.1psia, 13.637V, 0.315A, 257.276Ah, 4.29W",Subject: "Asleep until 16:29" @16:29:55.86
Powering core bus @16:29:56.16 upsync @16:29:59.54 upsync @16:30:01.25
Gate.power :raw,:ON @16:30:01.86 CS.configure :default @16:30:01.91 PS.configure :default @16:30:01.96 SS.configure :default @16:30:02.01 CC.configure :default @16:30:02.06 PC.configure :default @16:30:02.13 Forearm.configure :loose @16:30:02.17 Elbow.configure :loose @16:30:02.24 SC.configure :loose @16:30:02.29 SE.configure :default @16:30:02.33 CH.configure :default @16:30:02.37 PH.configure :default @16:30:02.41 All dwarf controllers running firmware version 3.85 @16:30:02.52 Valve.reset @16:30:02.81 Intake.select :close @16:30:02.85 PSV.dial :air @16:30:02.86 Exhaust.select :close @16:30:02.90 CTV.dial :air @16:30:03.04
Forearm.home.seek :retract @16:30:03.80 Hand.close @16:30:03.83
Elbow.reconfigure :loose @16:30:03.93 Elbow.jog 1500 @16:30:03.99 PS.home.seek 0ml @16:30:04.25 CSV.dial :waste1 @16:30:04.35
Elbow.reconfigure :loose @16:30:04.40 Elbow.home.seek :clear @16:30:04.99 SSV.dial :spit,avoiding: :exhaust @16:30:06.98 CS.reconfigure :fast @16:30:07.00 SS.home.seek 0ml @16:30:07.10 CS.home.seek 0ml @16:30:07.66
SE.home.seek :down @16:30:08.27 SC.reconfigure :tight @16:30:08.32 SC.home.seek :initial @16:30:12.21 PS.stop @16:30:36.70
SC.configure :limp @16:30:36.75 Forearm.seek :retract @16:30:36.82 Elbow.seek :hammer @16:30:37.86 Forearm.reconfigure :tight @16:30:37.91 Forearm.seek retracted @16:30:38.59 PC.home.seek :open @16:30:38.61
CC.home.seek :open @16:30:42.03 Phase [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] @16:30:42.05 Notice.email "At 16:30:41 began in tube 7: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_121923.rb:14\n{1 => 33,\n 2 => 33,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 7,\n 7 => 24}\nCTD data unavailable\nCan@16:30:42, 32% flow, 16.2C, 36% humidity, 15.1psia, 13.617V, 0.302A, 257.281Ah, 4.12W",Subject: "Started DA_WCR_121923 phase initialPurge; da [1000, 1000] #da and wsr are run simultanelously" @16:30:42.06
Starting initialPurge @16:30:42.09 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @16:30:42.15 Hand.open @16:30:42.94 Elbow.reconfigure :tight @16:30:42.99 Elbow.seek FlushPuck::Garage @16:30:44.79 Elbow.reconfigure :loose @16:30:44.89 Forearm.reconfigure :loose @16:30:44.94 Forearm.seek :garage @16:30:45.90 Hand.close @16:30:46.68 upsync @16:30:46.71
Forearm.seek :retract @16:30:47.68 Elbow.reconfigure :tight @16:30:47.73 Elbow.seek CC @16:30:50.67 Elbow.reconfigure :loose @16:30:50.78 Forearm.reconfigure :tight @16:30:50.83 Forearm.seek CC @16:30:52.09 CC.seek :closed @16:30:56.72 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.44 @16:30:56.79 Hand.open @16:30:57.48 Forearm.reconfigure :loose @16:30:57.53 Forearm.seek :retract @16:30:58.52 Flushing the Collection Valve::Manifold @16:30:58.64 CSV.dial CSR @16:30:59.94 Pulling 5ml of CSR.flush @16:30:59.95 CSR.select :flush @16:31:00.06 CS.reconfigure :default @16:31:00.52 CS.seek 5ml @16:31:14.34 delay 5 seconds @16:31:19.36 CSR.select :air @16:31:19.53 CS.reconfigure :fast @16:31:19.58 CS.seek 7.5ml @16:31:26.40 CSV.dial :waste @16:31:27.71 CS.reconfigure :default @16:31:27.75 CS.seek 0ml @16:31:47.84 Purging external Intake @16:31:47.97 CSV.dial :flush @16:31:48.43 CS.reconfigure :fast @16:31:48.47 CS.seek 10ml @16:32:13.37 delay 5 seconds @16:32:18.39 CSV.dial CBV @16:32:18.42
CBV.dial CSV @16:32:19.23 CTV.dial :intake @16:32:20.03 Intake.select :open @16:32:20.14 CS.seek 0ml @16:32:44.03 Intake.select :close @16:32:44.13 CTV.dial :air @16:32:44.92 Flushing upper manifold @16:32:45.04 CSV.dial :flush @16:32:45.78 CS.seek 5ml @16:32:58.72 delay 5 seconds @16:33:03.74 CSV.dial :air @16:33:04.29 CS.seek 6.999ml @16:33:09.97 CSV.dial CBV @16:33:09.99
CBV.dial CSV @16:33:11.04 CTV.dial :air @16:33:11.13 CS.seek 0ml @16:33:27.90 delay 7 seconds @16:33:34.93 CS.seek 3ml @16:33:43.06 CSV.dial :waste @16:33:43.52 CS.seek 0ml @16:33:51.36 CSV.dial :flush @16:33:51.80 CS.seek 3ml @16:34:00.03 delay 5 seconds @16:34:05.05 CSV.dial :air @16:34:05.56 CS.seek 5.001ml @16:34:11.12 CSV.dial :air @16:34:11.30 CS.seek 0ml @16:34:23.56 delay 7 seconds @16:34:30.58 CS.seek 3ml @16:34:38.59 CSV.dial :waste @16:34:39.33 CS.seek 0ml @16:34:47.03 Garaging FlushPuck in Collection Clamp @16:34:47.10 Elbow.reconfigure :tight @16:34:47.14 Elbow.seek CC @16:34:47.41 Elbow.reconfigure :loose @16:34:47.51 Forearm.seek CC @16:34:48.54 Hand.close @16:34:49.17 CC.seek :open @16:34:54.24 Forearm.seek :retract @16:34:55.22 Elbow.reconfigure :tight @16:34:55.27 Elbow.seek :garage @16:34:58.23 Elbow.reconfigure :loose @16:34:58.33 Forearm.reconfigure :tight @16:34:58.38 Forearm.seek :garage @16:34:59.38 Hand.open @16:35:00.11 Forearm.reconfigure :loose @16:35:00.15 Forearm.seek :retract @16:35:01.17 da sampling at most 1000ml, wcr at most 1000ml @16:35:01.21 Loading sh1 puck from tube 7 into Collection Clamp @16:35:01.22 DAfans.email "Started da in tube 7 at 16:35:01UTC19-Dec-23\n{1 => 33,\n 2 => 33,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 7,\n 7 => 24}\nCTD data unavailable\nCan@16:30:42, 32% flow, 16.2C, 36% humidity, 15.1psia, 13.617V, 0.302A, 257.281Ah, 4.12W",Subject: "da sampling at most 1000ml, wcr at most 1000ml" @16:35:01.51
SC.reconfigure :tight @16:35:01.56 SC.seek 7 @16:35:05.58 upsync @16:35:11.39
SC.configure :limp @16:35:11.62 Elbow.reconfigure :tight @16:35:11.67 Elbow.seek 7 @16:35:13.64 Forearm.reconfigure :tight @16:35:13.69 Forearm.seek 7 + 450 counts @16:35:14.74 SE.reconfigure :pick @16:35:14.80 SE.seek :beamBreak @16:35:16.41 SE.configure :default @16:35:16.57 Elbow.seek 7 @16:35:16.82 Forearm.seek 7 + 300 counts @16:35:17.06 Forearm.reconfigure :loose @16:35:17.11 Hand.close @16:35:17.69 SE.seek :down @16:35:18.27
Forearm.seek :retract @16:35:18.91 SE.stop @16:35:19.28
Elbow.seek CC @16:35:20.95 Elbow.reconfigure :loose @16:35:21.05 Forearm.reconfigure :tight @16:35:21.10 Forearm.seek CC @16:35:22.35 CC.seek :closed @16:35:26.94 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.28 @16:35:26.95 Hand.open @16:35:27.60 Forearm.reconfigure :loose @16:35:27.65 Forearm.seek :retract @16:35:28.66 collectSample 1000ml w/bubble point of 24psi @16:35:28.67 Priming 7ml @16:35:28.68 Intake.select :close @16:35:28.69 Exhaust.select :close @16:35:28.70 SS.hold @16:35:28.91 Calibrating pressure and temperature sensors @16:35:28.94 CTV.dial :air @16:35:29.01 SSV.dial :spit,avoiding: :exhaust @16:35:29.09 SS.reconfigure :fast @16:35:29.14 SS.seek 5ml @16:35:32.01 SSV.dial CBV @16:35:32.05
CBV.dial SSV @16:35:33.38 delay 3.8 seconds @16:35:37.31 Sensor limits: Intake max 98.7psia; Outlet max 92.3psia @16:35:37.32 SSV.dial :spit @16:35:38.65 SS.seek 0ml @16:35:41.50 SSV.dial CBV @16:35:42.84 CTV.dial :intake @16:35:43.64 SSV.dial CBV @16:35:43.66
CBV.dial SSV @16:35:43.80 Allowing 40s for 7.0ml of remaining 7.0ml @16:35:43.82 Intake.select :open @16:35:45.98 Intake @14.4psia, vacuum from 8.9 to 11.9psi @16:35:46.00 SS.reconfigure :inhaling 2.20ml/s @16:35:46.04 SS.seek 7ml @16:35:52.88 Stroke completed -- waiting for pressure to equalize ... @16:35:52.89 SS.reconfigure :exhale @16:35:52.93 SS.hold @16:35:58.78 Intake.select :close @16:35:58.94 SSV.dial :exhaust @16:35:59.72 Exhaust.select :open @16:35:59.84 SS.seek 0ml @16:36:04.07 Exhaust.select :close @16:36:04.17 Sampling 1000.0ml @16:36:04.18 Intake.select :close @16:36:04.19 Exhaust.select :close @16:36:04.21 SS.reconfigure :default @16:36:04.25 SS.hold @16:36:04.45 CTV.dial :intake @16:36:04.62 SSV.dial CBV @16:36:04.64
CBV.dial SSV @16:36:05.44 Allowing 130s for 25.0ml of remaining 1000.0ml @16:36:05.45 Intake.select :open @16:36:07.62 Intake @15.1psia, vacuum from 9.6 to 12.6psi @16:36:07.63 SS.reconfigure :inhaling 2.20ml/s @16:36:07.68 SS.seek 25ml @16:36:22.67 Stroke completed -- waiting for pressure to equalize ... @16:36:22.68 SS.reconfigure :exhale @16:36:22.73 SS.hold @16:36:47.29 Intake.select :close @16:36:47.44 SSV.dial :exhaust @16:36:48.21 Exhaust.select :open @16:36:48.32 SS.seek 0ml @16:36:57.08 Exhaust.select :close @16:36:57.19 SSV.dial CBV @16:36:57.21
CBV.dial SSV @16:36:58.01 Allowing 130s for 25.0ml of remaining 975.0ml @16:36:58.02 Intake.select :open @16:37:00.18 Intake @15.6psia, vacuum from 10.0 to 13.0psi @16:37:00.19 SS.reconfigure :inhaling 2.20ml/s @16:37:00.24 SS.seek 25ml @16:37:14.43 Vacuum too high -- waiting up to 40 seconds @16:37:14.45 SS.reconfigure :exhale @16:37:14.50 SS.hold @16:37:20.53 Intake @17.6psia, vacuum from 10.0 to 13.0psi @16:37:20.54 SS.reconfigure :inhaling 1.76ml/s @16:37:20.59 SS.seek 25ml @16:37:21.77 Stroke completed -- waiting for pressure to equalize ... @16:37:21.78 SS.reconfigure :exhale @16:37:21.82 SS.hold @16:37:44.74 Intake.select :close @16:37:44.89 SSV.dial :exhaust @16:37:45.67 Exhaust.select :open @16:37:45.78 SS.seek 0ml @16:37:54.53 Exhaust.select :close @16:37:54.64 SSV.dial CBV @16:37:54.66
CBV.dial SSV @16:37:55.47 Allowing 130s for 25.0ml of remaining 950.0ml @16:37:55.48 Intake.select :open @16:37:57.65 Intake @15.7psia, vacuum from 10.0 to 13.0psi @16:37:57.66 SS.reconfigure :inhaling 1.76ml/s @16:37:57.71 SS.seek 25ml @16:38:15.20 Stroke completed -- waiting for pressure to equalize ... @16:38:15.21 SS.reconfigure :exhale @16:38:15.26 SS.hold @16:38:42.01 Intake.select :close @16:38:42.16 SSV.dial :exhaust @16:38:42.94 Exhaust.select :open @16:38:43.05 SS.seek 0ml @16:38:51.73 Exhaust.select :close @16:38:51.85 SSV.dial CBV @16:38:51.87
CBV.dial SSV @16:38:52.66 Allowing 130s for 25.0ml of remaining 925.0ml @16:38:52.67 Intake.select :open @16:38:54.83 Intake @15.8psia, vacuum from 10.0 to 13.0psi @16:38:54.84 SS.reconfigure :inhaling 1.76ml/s @16:38:54.89 SS.seek 25ml @16:39:12.17 Stroke completed -- waiting for pressure to equalize ... @16:39:12.18 SS.reconfigure :exhale @16:39:12.23 SS.hold @16:39:34.76 Intake.select :close @16:39:34.92 SSV.dial :exhaust @16:39:35.67 Exhaust.select :open @16:39:35.79 SS.seek 0ml @16:39:44.56 Exhaust.select :close @16:39:44.67 SSV.dial CBV @16:39:44.69
CBV.dial SSV @16:39:45.50 Allowing 130s for 25.0ml of remaining 900.0ml @16:39:45.51 Intake.select :open @16:39:47.68 Intake @16.0psia, vacuum from 10.0 to 13.0psi @16:39:47.69 SS.reconfigure :inhaling 1.76ml/s @16:39:47.74 SS.seek 25ml @16:40:05.03 Stroke completed -- waiting for pressure to equalize ... @16:40:05.04 SS.reconfigure :exhale @16:40:05.08 SS.hold @16:40:28.28 Intake.select :close @16:40:28.43 SSV.dial :exhaust @16:40:29.20 Exhaust.select :open @16:40:29.36 SS.seek 0ml @16:40:38.12 Exhaust.select :close @16:40:38.23 SSV.dial CBV @16:40:38.25
CBV.dial SSV @16:40:39.05 Allowing 130s for 25.0ml of remaining 875.0ml @16:40:39.06 Intake.select :open @16:40:41.22 Intake @15.9psia, vacuum from 10.0 to 13.0psi @16:40:41.23 SS.reconfigure :inhaling 1.76ml/s @16:40:41.28 SS.seek 25ml @16:40:58.56 Stroke completed -- waiting for pressure to equalize ... @16:40:58.57 SS.reconfigure :exhale @16:40:58.62 SS.hold @16:41:21.36 Intake.select :close @16:41:21.51 SSV.dial :exhaust @16:41:22.26 Exhaust.select :open @16:41:22.38 SS.seek 0ml @16:41:31.06 Exhaust.select :close @16:41:31.18 SSV.dial CBV @16:41:31.20
CBV.dial SSV @16:41:31.98 Allowing 130s for 25.0ml of remaining 850.0ml @16:41:31.99 Intake.select :open @16:41:34.16 Intake @16.0psia, vacuum from 10.0 to 13.0psi @16:41:34.18 SS.reconfigure :inhaling 1.76ml/s @16:41:34.23 SS.seek 25ml @16:41:48.80 Vacuum too high -- waiting up to 40 seconds @16:41:48.81 SS.reconfigure :exhale @16:41:48.87 SS.hold @16:41:54.43 Intake @16.2psia, vacuum from 10.0 to 13.0psi @16:41:54.45 SS.reconfigure :inhaling 1.41ml/s @16:41:54.50 SS.seek 25ml @16:41:58.54 Stroke completed -- waiting for pressure to equalize ... @16:41:58.55 SS.reconfigure :exhale @16:41:58.60 SS.hold @16:42:15.12 Intake.select :close @16:42:15.28 SSV.dial :exhaust @16:42:16.06 Exhaust.select :open @16:42:16.17 SS.seek 0ml @16:42:25.12 Exhaust.select :close @16:42:25.23 SSV.dial CBV @16:42:25.25
CBV.dial SSV @16:42:26.03 Allowing 130s for 25.0ml of remaining 825.0ml @16:42:26.04 Intake.select :open @16:42:28.20 Intake @15.9psia, vacuum from 10.0 to 13.0psi @16:42:28.22 SS.reconfigure :inhaling 1.41ml/s @16:42:28.26 SS.seek 25ml @16:42:48.51 Stroke completed -- waiting for pressure to equalize ... @16:42:48.52 SS.reconfigure :exhale @16:42:48.57 SS.hold @16:43:07.02 Intake.select :close @16:43:07.18 SSV.dial :exhaust @16:43:07.93 Exhaust.select :open @16:43:08.05 SS.seek 0ml @16:43:16.92 Exhaust.select :close @16:43:17.03 SSV.dial CBV @16:43:17.05
CBV.dial SSV @16:43:17.82 Allowing 130s for 25.0ml of remaining 800.0ml @16:43:17.83 Intake.select :open @16:43:20.00 Intake @15.9psia, vacuum from 10.0 to 13.0psi @16:43:20.01 SS.reconfigure :inhaling 1.41ml/s @16:43:20.06 SS.seek 25ml @16:43:40.27 Stroke completed -- waiting for pressure to equalize ... @16:43:40.28 SS.reconfigure :exhale @16:43:40.33 SS.hold @16:44:00.76 Intake.select :close @16:44:00.91 SSV.dial :exhaust @16:44:01.67 Exhaust.select :open @16:44:01.79 SS.seek 0ml @16:44:10.79 Exhaust.select :close @16:44:10.90 SSV.dial CBV @16:44:10.92
CBV.dial SSV @16:44:11.71 Allowing 130s for 25.0ml of remaining 775.0ml @16:44:11.72 Intake.select :open @16:44:13.89 Intake @15.9psia, vacuum from 10.0 to 13.0psi @16:44:13.90 SS.reconfigure :inhaling 1.41ml/s @16:44:13.95 SS.seek 25ml @16:44:34.01 Stroke completed -- waiting for pressure to equalize ... @16:44:34.02 SS.reconfigure :exhale @16:44:34.06 SS.hold @16:44:52.74 Intake.select :close @16:44:52.90 SSV.dial :exhaust @16:44:53.67 Exhaust.select :open @16:44:53.78 SS.seek 0ml @16:45:02.49 Exhaust.select :close @16:45:02.60 SSV.dial CBV @16:45:02.63
CBV.dial SSV @16:45:03.40 Allowing 130s for 25.0ml of remaining 750.0ml @16:45:03.41 Intake.select :open @16:45:05.58 Intake @15.9psia, vacuum from 10.0 to 13.0psi @16:45:05.59 SS.reconfigure :inhaling 1.41ml/s @16:45:05.64 SS.seek 25ml @16:45:26.37 Stroke completed -- waiting for pressure to equalize ... @16:45:26.38 SS.reconfigure :exhale @16:45:26.42 SS.hold @16:45:46.81 Intake.select :close @16:45:47.72 SSV.dial :exhaust @16:45:48.48 Exhaust.select :open @16:45:48.59 SS.seek 0ml @16:45:57.38 Exhaust.select :close @16:45:57.50 SSV.dial CBV @16:45:57.53
CBV.dial SSV @16:45:58.27 Allowing 130s for 25.0ml of remaining 725.0ml @16:45:58.28 Intake.select :open @16:46:00.45 Intake @16.0psia, vacuum from 10.0 to 13.0psi @16:46:00.47 SS.reconfigure :inhaling 1.41ml/s @16:46:00.52 SS.seek 25ml @16:46:19.33 Vacuum too high -- waiting up to 40 seconds @16:46:19.34 SS.reconfigure :exhale @16:46:19.39 SS.hold @16:46:27.01 Intake @15.1psia, vacuum from 9.6 to 12.6psi @16:46:27.03 SS.reconfigure :inhaling 1.13ml/s @16:46:27.08 SS.seek 25ml @16:46:29.56 Stroke completed -- waiting for pressure to equalize ... @16:46:29.57 SS.reconfigure :exhale @16:46:29.62 SS.hold @16:46:40.97 Intake.select :close @16:46:41.13 SSV.dial :exhaust @16:46:41.88 Exhaust.select :open @16:46:42.00 SS.seek 0ml @16:46:50.99 Exhaust.select :close @16:46:51.10 SSV.dial CBV @16:46:51.12
CBV.dial SSV @16:46:51.93 Allowing 130s for 25.0ml of remaining 700.0ml @16:46:51.94 Intake.select :open @16:46:54.11 Intake @16.0psia, vacuum from 10.0 to 13.0psi @16:46:54.12 SS.reconfigure :inhaling 1.13ml/s @16:46:54.17 SS.seek 25ml @16:47:17.17 Vacuum too high -- waiting up to 40 seconds @16:47:17.18 SS.reconfigure :exhale @16:47:17.24 SS.hold @16:47:26.34 Intake @16.3psia, vacuum from 10.0 to 13.0psi @16:47:26.36 SS.reconfigure :inhaling 0.91ml/s @16:47:26.41 SS.seek 25ml @16:47:28.01 Stroke completed -- waiting for pressure to equalize ... @16:47:28.02 SS.reconfigure :exhale @16:47:28.06 SS.hold @16:47:40.80 Intake.select :close @16:47:40.95 SSV.dial :exhaust @16:47:41.71 Exhaust.select :open @16:47:41.83 SS.seek 0ml @16:47:50.54 Exhaust.select :close @16:47:50.64 SSV.dial CBV @16:47:50.66
CBV.dial SSV @16:47:51.43 Allowing 130s for 25.0ml of remaining 675.0ml @16:47:51.44 Intake.select :open @16:47:53.61 Intake @15.9psia, vacuum from 10.0 to 13.0psi @16:47:53.62 SS.reconfigure :inhaling 0.91ml/s @16:47:53.67 SS.seek 25ml @16:48:23.88 Stroke completed -- waiting for pressure to equalize ... @16:48:23.89 SS.reconfigure :exhale @16:48:23.94 SS.hold @16:48:44.60 Intake.select :close @16:48:44.76 SSV.dial :exhaust @16:48:45.52 Exhaust.select :open @16:48:45.64 SS.seek 0ml @16:48:54.57 Exhaust.select :close @16:48:54.68 SSV.dial CBV @16:48:54.70
CBV.dial SSV @16:48:55.52 Allowing 130s for 25.0ml of remaining 650.0ml @16:48:55.53 Intake.select :open @16:48:57.71 Intake @15.9psia, vacuum from 10.0 to 13.0psi @16:48:57.72 SS.reconfigure :inhaling 0.91ml/s @16:48:57.77 SS.seek 25ml @16:49:27.27 Stroke completed -- waiting for pressure to equalize ... @16:49:27.28 SS.reconfigure :exhale @16:49:27.33 SS.hold @16:49:51.88 Intake.select :close @16:49:52.04 SSV.dial :exhaust @16:49:52.80 Exhaust.select :open @16:49:52.92 SS.seek 0ml @16:50:01.54 Exhaust.select :close @16:50:01.64 SSV.dial CBV @16:50:01.66
CBV.dial SSV @16:50:02.44 Allowing 130s for 25.0ml of remaining 625.0ml @16:50:02.45 Intake.select :open @16:50:04.62 Intake @15.9psia, vacuum from 10.0 to 13.0psi @16:50:04.64 SS.reconfigure :inhaling 0.91ml/s @16:50:04.69 SS.seek 25ml @16:50:27.01 Vacuum too high -- waiting up to 40 seconds @16:50:27.02 SS.reconfigure :exhale @16:50:27.07 SS.hold @16:50:38.76 Intake @16.7psia, vacuum from 10.0 to 13.0psi @16:50:38.77 SS.reconfigure :inhaling 0.73ml/s @16:50:38.82 SS.seek 25ml @16:50:48.43 Stroke completed -- waiting for pressure to equalize ... @16:50:48.44 SS.reconfigure :exhale @16:50:48.48 SS.hold @16:51:05.11 Intake.select :close @16:51:05.26 SSV.dial :exhaust @16:51:06.02 Exhaust.select :open @16:51:06.14 SS.seek 0ml @16:51:15.07 Exhaust.select :close @16:51:15.17 SSV.dial CBV @16:51:15.19
CBV.dial SSV @16:51:15.97 Allowing 130s for 25.0ml of remaining 600.0ml @16:51:15.98 Intake.select :open @16:51:18.15 Intake @16.0psia, vacuum from 10.0 to 13.0psi @16:51:18.17 SS.reconfigure :inhaling 0.73ml/s @16:51:18.22 SS.seek 25ml @16:51:43.89 Vacuum too high -- waiting up to 40 seconds @16:51:43.90 SS.reconfigure :exhale @16:51:43.95 SS.hold @16:51:52.62 Intake @15.3psia, vacuum from 9.8 to 12.8psi @16:51:52.63 SS.reconfigure :inhaling 0.59ml/s @16:51:52.68 SS.seek 25ml @16:52:05.77 Stroke completed -- waiting for pressure to equalize ... @16:52:05.78 SS.reconfigure :exhale @16:52:05.82 SS.hold @16:52:26.27 Intake.select :close @16:52:26.42 SSV.dial :exhaust @16:52:27.18 Exhaust.select :open @16:52:27.30 SS.seek 0ml @16:52:36.10 Exhaust.select :close @16:52:36.20 SSV.dial CBV @16:52:36.22
CBV.dial SSV @16:52:37.00 Allowing 130s for 25.0ml of remaining 575.0ml @16:52:37.01 Intake.select :open @16:52:39.18 Intake @15.8psia, vacuum from 10.0 to 13.0psi @16:52:39.19 SS.reconfigure :inhaling 0.59ml/s @16:52:39.24 SS.seek 25ml @16:53:24.05 Stroke completed -- waiting for pressure to equalize ... @16:53:24.06 SS.reconfigure :exhale @16:53:24.10 SS.hold @16:53:50.67 Intake.select :close @16:53:50.82 SSV.dial :exhaust @16:53:51.59 Exhaust.select :open @16:53:51.71 SS.seek 0ml @16:54:00.41 Exhaust.select :close @16:54:00.51 SSV.dial CBV @16:54:00.53
CBV.dial SSV @16:54:01.33 Allowing 130s for 25.0ml of remaining 550.0ml @16:54:01.34 Intake.select :open @16:54:03.51 Intake @15.9psia, vacuum from 10.0 to 13.0psi @16:54:03.52 SS.reconfigure :inhaling 0.59ml/s @16:54:03.57 SS.seek 25ml @16:54:46.73 Vacuum too high -- waiting up to 40 seconds @16:54:46.74 SS.reconfigure :exhale @16:54:46.79 SS.hold @16:55:05.68 Intake @17.2psia, vacuum from 10.0 to 13.0psi @16:55:05.69 SS.reconfigure :inhaling 0.47ml/s @16:55:05.74 SS.seek 25ml @16:55:08.36 Stroke completed -- waiting for pressure to equalize ... @16:55:08.37 SS.reconfigure :exhale @16:55:08.41 SS.hold @16:55:23.12 Intake.select :close @16:55:23.27 SSV.dial :exhaust @16:55:24.03 Exhaust.select :open @16:55:24.15 SS.seek 0ml @16:55:32.91 Exhaust.select :close @16:55:33.01 SSV.dial CBV @16:55:33.03
CBV.dial SSV @16:55:33.85 Allowing 130s for 25.0ml of remaining 525.0ml @16:55:33.86 Intake.select :open @16:55:36.02 Intake @15.8psia, vacuum from 10.0 to 13.0psi @16:55:36.03 SS.reconfigure :inhaling 0.47ml/s @16:55:36.08 SS.seek 25ml @16:56:31.73 Stroke completed -- waiting for pressure to equalize ... @16:56:31.74 SS.reconfigure :exhale @16:56:31.79 SS.hold @16:57:04.45 Intake.select :close @16:57:04.61 SSV.dial :exhaust @16:57:05.38 Exhaust.select :open @16:57:05.50 SS.seek 0ml @16:57:14.31 Exhaust.select :close @16:57:14.47 SSV.dial CBV @16:57:14.54
CBV.dial SSV @16:57:15.33 Allowing 130s for 25.0ml of remaining 500.0ml @16:57:15.34 Intake.select :open @16:57:17.50 Intake @15.9psia, vacuum from 10.0 to 13.0psi @16:57:17.51 SS.reconfigure :inhaling 0.47ml/s @16:57:17.56 SS.seek 25ml @16:58:03.56 Vacuum too high -- waiting up to 40 seconds @16:58:03.58 SS.reconfigure :exhale @16:58:03.63 SS.hold @16:58:20.35 Intake @16.6psia, vacuum from 10.0 to 13.0psi @16:58:20.36 SS.reconfigure :inhaling 0.38ml/s @16:58:20.41 SS.seek 25ml @16:58:32.70 Stroke completed -- waiting for pressure to equalize ... @16:58:32.71 SS.reconfigure :exhale @16:58:32.76 SS.hold @16:58:55.18 Intake.select :close @16:58:55.34 SSV.dial :exhaust @16:58:56.11 Exhaust.select :open @16:58:56.22 SS.seek 0ml @16:59:05.23 Exhaust.select :close @16:59:05.34 SSV.dial CBV @16:59:05.36
CBV.dial SSV @16:59:06.12 Allowing 130s for 25.0ml of remaining 475.0ml @16:59:06.13 Intake.select :open @16:59:08.30 Intake @15.9psia, vacuum from 10.0 to 13.0psi @16:59:08.31 SS.reconfigure :inhaling 0.38ml/s @16:59:08.36 SS.seek 25ml @17:00:17.63 Stroke completed -- waiting for pressure to equalize ... @17:00:17.64 SS.reconfigure :exhale @17:00:17.69 SS.hold @17:00:54.44 Intake.select :close @17:00:54.59 SSV.dial :exhaust @17:00:55.43 Exhaust.select :open @17:00:55.54 SS.seek 0ml @17:01:04.32 Exhaust.select :close @17:01:04.43 SSV.dial CBV @17:01:04.46
CBV.dial SSV @17:01:05.23 Allowing 130s for 25.0ml of remaining 450.0ml @17:01:05.24 Intake.select :open @17:01:07.41 Intake @15.8psia, vacuum from 10.0 to 13.0psi @17:01:07.42 SS.reconfigure :inhaling 0.38ml/s @17:01:07.48 SS.seek 25ml @17:02:14.31 Vacuum too high -- waiting up to 40 seconds @17:02:14.32 SS.reconfigure :exhale @17:02:14.38 SS.hold @17:02:36.94 Intake @16.8psia, vacuum from 10.0 to 13.0psi @17:02:36.96 SS.reconfigure :inhaling 0.31ml/s @17:02:37.00 SS.seek 25ml @17:02:40.25 Stroke completed -- waiting for pressure to equalize ... @17:02:40.27 SS.reconfigure :exhale @17:02:40.31 SS.hold @17:03:03.16 Intake.select :close @17:03:03.32 SSV.dial :exhaust @17:03:04.09 Exhaust.select :open @17:03:04.21 SS.seek 0ml @17:03:13.05 Exhaust.select :close @17:03:13.16 SSV.dial CBV @17:03:13.18
CBV.dial SSV @17:03:13.99 Allowing 130s for 25.0ml of remaining 425.0ml @17:03:14.00 Intake.select :open @17:03:16.17 Intake @15.9psia, vacuum from 10.0 to 13.0psi @17:03:16.18 SS.reconfigure :inhaling 0.31ml/s @17:03:16.23 SS.seek 25ml @17:04:45.39 Stroke completed -- waiting for pressure to equalize ... @17:04:45.40 SS.reconfigure :exhale @17:04:45.45 SS.hold @17:05:24.01 SS.reconfigure :default @17:05:24.06 SS.hold @17:05:24.12 Intake.select :close @17:05:24.28 ShallowSampler::Clogged in MAIN -- Filtering too Slowly after 600.0ml @17:05:24.29 SSV.dial :exhaust @17:05:25.05 Exhaust.select :open @17:05:25.16 SS.reconfigure :exhale @17:05:25.21 SS.seek 0ml @17:05:34.66 Exhaust.select :close @17:05:34.77 Sampled 600.0ml @17:05:34.78 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @17:05:34.79 Intake.select :close @17:05:34.86 CSV.dial :waste @17:05:35.01 SSV.dial CBV,avoiding: :exhaust @17:05:35.04 CS.seek 0ml @17:05:35.08
CBV.dial SSV @17:05:35.24 CSV.dial :air @17:05:35.99 CS.seek 10ml @17:05:37.52
CTV.dial :air @17:05:38.30 delay 15 seconds @17:05:53.32 SSV.dial :spit,avoiding: :exhaust @17:05:54.64 CTV.dial CSV,avoiding: :intake @17:05:54.67
SS.seek 0ml @17:05:54.80 SSV.dial CBV,avoiding: :exhaust @17:06:00.59 SSV.dial between CBV and plug @17:06:01.09 CBV.dial between SSV and waste2 @17:06:01.48 CSV.dial between air and CTV @17:06:01.80 CS.seek 8.38ml @17:06:06.35 CTV.dial CSV,avoiding: :air @17:06:06.37
CSV.dial CTV @17:06:06.71 CS.reconfigure :pressurize @17:06:06.76 CS.seek 0ml @17:06:20.53 BubblePoint: Intake @24.1psia, Outlet @0.8psia @17:06:20.54 CBV.dial SSV @17:06:20.89 SS.reconfigure :default @17:06:20.94 SS.seek 10ml @17:06:26.52 delay 15 seconds @17:06:41.65 Evacuated: Intake @23.6psia, Outlet @23.2psia @17:06:41.66 SSV.dial :spit,avoiding: :exhaust @17:06:42.70 SS.reconfigure :exhale @17:06:42.74 SS.seek 0ml @17:06:47.71 SS.reconfigure :fast @17:06:47.75 SS.seek 5ml @17:06:50.51 SSV.dial CBV,avoiding: :exhaust @17:06:50.53
CBV.dial SSV @17:06:51.86 CTV.dial :air,avoiding: :intake @17:06:53.23 CSV.dial CTV @17:06:53.29
SS.seek 10ml @17:06:54.44 CSV.dial :air @17:06:54.88 CS.reconfigure :fast @17:06:54.93 CS.seek 0ml @17:06:55.78
SSV.dial :spit,avoiding: :exhaust @17:06:57.16 SS.reconfigure :exhale @17:06:57.21 SS.seek 0ml @17:07:02.23 SS.reconfigure :fast @17:07:02.28 SS.seek 5ml @17:07:03.18 CS.stop @17:07:05.10 SSV.dial CBV,avoiding: :exhaust @17:07:05.12
CBV.dial SSV @17:07:07.48 SSV.dial :spit,avoiding: :exhaust @17:07:08.79 SS.reconfigure :default @17:07:08.84 SS.seek 0ml @17:07:12.05 SS.stop @17:07:12.09 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @17:07:12.10 Intake.select :close @17:07:12.17 CSV.dial :waste @17:07:12.30 SSV.dial CBV,avoiding: :exhaust @17:07:12.32
CBV.dial SSV @17:07:12.94 CS.seek 0ml @17:07:13.00 CSV.dial :air @17:07:13.71
CTV.dial :air @17:07:13.78 CS.seek 10ml @17:07:13.89
delay 15 seconds @17:07:28.91 SSV.dial :spit,avoiding: :exhaust @17:07:30.23 CTV.dial CSV,avoiding: :intake @17:07:30.26
SS.reconfigure :exhale @17:07:30.36 SS.seek 0ml @17:07:30.74 SSV.dial CBV,avoiding: :exhaust @17:07:38.34 SSV.dial between CBV and plug @17:07:38.82 CBV.dial between SSV and waste2 @17:07:39.23 CSV.dial between air and CTV @17:07:39.54 CS.seek 7.316ml @17:07:46.38 CTV.dial CSV,avoiding: :air @17:07:46.40
CSV.dial CTV @17:07:46.75 CS.reconfigure :pressurize @17:07:46.80 CS.seek 0ml @17:08:01.82 BubblePoint: Intake @24.3psia, Outlet @3.5psia @17:08:01.83 CBV.dial SSV @17:08:02.16 SS.reconfigure :default @17:08:02.21 SS.seek 10ml @17:08:07.80 delay 15 seconds @17:08:22.93 Evacuated: Intake @24.0psia, Outlet @23.5psia @17:08:22.94 SSV.dial :spit,avoiding: :exhaust @17:08:23.99 SS.reconfigure :exhale @17:08:24.04 SS.seek 0ml @17:08:29.03 SS.reconfigure :fast @17:08:29.07 SS.seek 5ml @17:08:32.24 SSV.dial CBV,avoiding: :exhaust @17:08:32.26
CBV.dial SSV @17:08:33.64 CTV.dial :air,avoiding: :intake @17:08:34.97 CSV.dial CTV @17:08:35.01
SS.seek 10ml @17:08:36.21 CSV.dial :air @17:08:36.66 CS.reconfigure :fast @17:08:36.71 CS.seek 0ml @17:08:37.52
SSV.dial :spit,avoiding: :exhaust @17:08:38.86 SS.reconfigure :exhale @17:08:38.91 SS.seek 0ml @17:08:41.34 CS.stop @17:08:43.90
SS.reconfigure :fast @17:08:43.95 SS.seek 5ml @17:08:46.88 SSV.dial CBV,avoiding: :exhaust @17:08:46.90
CBV.dial SSV @17:08:49.23 SSV.dial :spit,avoiding: :exhaust @17:08:50.57 SS.reconfigure :default @17:08:50.62 SS.seek 0ml @17:08:53.87 SS.stop @17:08:53.91 Lysing: Adding 2.0ml AqMeOH @17:08:53.93 CBV.dial between CSV and waste1,avoiding: :DBV @17:08:55.43 CTV.dial CSV @17:08:56.76 CSV.dial CSR @17:08:57.68 Pulling 1ml of CSR.AqMeOH @17:08:57.69 CSR.select :AqMeOH @17:08:57.80 CS.reconfigure :default @17:08:57.85 CS.seek 1ml @17:09:01.40 delay 5 seconds @17:09:06.42 CSR.select :air @17:09:06.59 CS.seek 6ml @17:09:20.18 delay 2 seconds @17:09:22.20 CSV.dial :waste2 @17:09:22.78 CS.reconfigure :fast @17:09:22.83 CS.seek 0ml @17:09:37.24 CSV.dial CSR @17:09:37.68 Pulling 2ml of CSR.AqMeOH @17:09:37.69 CSR.select :AqMeOH @17:09:37.80 CS.reconfigure :default @17:09:37.85 CS.seek 2ml @17:09:44.09 delay 5 seconds @17:09:49.11 CSR.select :air @17:09:49.32 CS.seek 5ml @17:09:58.02 delay 2 seconds @17:10:00.04 CSV.dial CTV @17:10:00.50 CS.seek 1ml @17:10:11.85 Before lysing Collection Puck Heater at 13.8C/30.5psia @17:10:11.87 Heating Puck.from 7, :sh1 in Collection Clamp to 70C for 10:00 @17:10:11.89 CH.seek 70C,8:00 @17:12:28.43 CH.hold 10:00,70C @17:12:28.64 Begun lysing Collection Puck Heater at 68.1C/37.4psia @17:22:28.64 Ended lysing Collection Puck Heater at 70C/36.3psia @17:22:28.66 CS.seek 0ml @17:22:32.14 CTV.dial between spare and air,avoiding: :intake @17:22:33.17 CSV.dial :air @17:22:33.62 CS.seek 5ml @17:22:47.23 CSV.dial CTV @17:22:47.69 delay 2 seconds @17:22:49.70 CSV.dial :air @17:22:50.15 delay 2 seconds @17:22:52.17 CSV.dial CTV @17:22:52.63 delay 2 seconds @17:22:54.65 CSV.dial :air @17:22:55.09 delay 2 seconds @17:22:57.11 CS.seek 0ml @17:23:10.78 Puck @80.0psia after final lysis pressurization @17:23:10.80 Recovering Lysate into Collection syringe @17:23:10.81 CSV.dial CBV @17:23:11.83 CBV.dial CSV @17:23:12.22 CS.seek 5ml @17:23:25.83 delay 5 seconds @17:23:30.84 CTV.dial :air @17:23:31.37 CS.seek 7ml @17:23:37.29 delay 5 seconds @17:23:42.31 CSV.dial :air2 @17:23:42.77 CS.seek 10ml @17:23:51.20 CSV.dial between air and CTV,avoiding: CBV @17:23:52.36 Lysing Complete @17:23:52.43 Storing sh1 puck in Collection Clamp to tube 6 @17:23:52.60 SC.reconfigure :tight @17:23:52.65 SC.seek 6 @17:23:56.23 SC.configure :limp @17:23:56.44 Elbow.reconfigure :tight @17:23:56.49 Elbow.seek 6 @17:23:58.70 Forearm.reconfigure :tight @17:23:58.75 Forearm.seek 6 + 450 counts @17:23:59.74 SE.reconfigure :pick @17:23:59.80 SE.seek :beamBreak @17:24:02.54 SE.configure :default @17:24:02.70 Elbow.reconfigure :limp @17:24:02.75 Forearm.reconfigure :loose @17:24:02.79 Forearm.seek 6 @17:24:03.01 Elbow.hold @17:24:03.07 Elbow.reconfigure :tight @17:24:03.17 SE.seek 6.16 Pucks below Top Plate @17:24:03.71 Forearm.force -20 @17:24:03.75 Forearm.seek 6 + 301 counts @17:24:04.03 Forearm.reconfigure :tight @17:24:04.08 Forearm.seek 6 + 450 counts @17:24:04.26 SE.seek 7.16 Pucks below Top Plate @17:24:04.79 Forearm.reconfigure :loose @17:24:04.83 Forearm.seek :retract @17:24:05.77 Elbow.seek CC @17:24:07.47 Elbow.reconfigure :loose @17:24:07.57 Forearm.seek CC @17:24:08.55 Hand.close @17:24:09.14 CC.seek :open @17:24:13.87 Forearm.seek :retract @17:24:14.84 Elbow.reconfigure :tight @17:24:14.89 Elbow.seek 6 @17:24:16.64 Elbow.reconfigure :limp @17:24:16.69 Forearm.seek 6 @17:24:18.10 Elbow.hold @17:24:18.23 Elbow.reconfigure :tight @17:24:18.28 Hand.open @17:24:19.00 Forearm.force -20 @17:24:19.05 Forearm.seek 6 + 314 counts @17:24:19.33 Forearm.reconfigure :tight @17:24:19.38 Forearm.seek 6 + 450 counts @17:24:19.64 SE.seek :down @17:24:20.77
Forearm.reconfigure :loose @17:24:20.82 Forearm.seek :retract @17:24:21.84 SE.stop @17:24:21.89
Tranferring DA @17:24:21.90 Beginning samplePassC @17:24:21.92 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @17:24:22.10 Elbow.seek FlushPuck::Garage @17:24:23.95 Elbow.reconfigure :loose @17:24:24.05 Forearm.seek :garage @17:24:25.04 Hand.close @17:24:25.75 Forearm.seek :retract @17:24:26.76 Elbow.reconfigure :tight @17:24:26.81 Elbow.seek CC @17:24:29.72 Elbow.reconfigure :loose @17:24:29.82 Forearm.reconfigure :tight @17:24:29.87 Forearm.seek CC @17:24:31.09 CC.seek :closed @17:24:35.62 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.40 @17:24:35.68 Hand.open @17:24:36.32 Forearm.reconfigure :loose @17:24:36.37 Forearm.seek :retract @17:24:37.37 PSV.dial PSR @17:24:38.76 Pulling 1.5ml of PSR.flush @17:24:38.77 PSR.select :flush @17:24:38.90 PS.seek 1.5ml @17:24:55.02 delay 5 seconds @17:25:00.04 PSV.dial :waste1 @17:25:00.51 PS.seek 0ml @17:25:16.70 PSV.dial PSR @17:25:17.19 PSR.select :air @17:25:17.30 PS.seek 2.5ml @17:25:43.59 PRV.dial CBV,avoiding: :ab2 @17:25:43.61
CBV.dial PRV @17:25:45.38 PSV.dial :mixloop @17:25:46.43 CTV.dial :air,avoiding: :intake @17:25:46.49 PTV.dial :PRVmixing @17:25:47.82 PS.seek 0ml @17:26:14.17 PSV.dial :air @17:26:14.65 PS.seek 2.5ml @17:26:40.95 PSV.dial :mixloop @17:26:41.44 PS.seek 0ml @17:27:07.86 PSV.dial :air @17:27:08.33 PS.seek 2.5ml @17:27:34.73 PSV.dial :mixloop @17:27:35.21 PS.seek 0ml @17:28:01.74 Garaging FlushPuck in Collection Clamp @17:28:01.81 Elbow.reconfigure :tight @17:28:01.85 Elbow.seek CC @17:28:02.13 Elbow.reconfigure :loose @17:28:02.23 Forearm.seek CC @17:28:03.24 Hand.close @17:28:03.88 CC.seek :open @17:28:08.98 Forearm.seek :retract @17:28:09.93 Elbow.reconfigure :tight @17:28:09.99 Elbow.seek :garage @17:28:12.89 Elbow.reconfigure :loose @17:28:13.00 Forearm.reconfigure :tight @17:28:13.04 Forearm.seek :garage @17:28:14.10 Hand.open @17:28:14.81 Forearm.reconfigure :loose @17:28:14.86 Forearm.seek :retract @17:28:15.86 PSV.dial :mixloop @17:28:15.94 PTV.dial :PRVmixing @17:28:15.95 PRV.dial CBV @17:28:15.98
CBV.dial PRV @17:28:16.21 CTV.dial :air,avoiding: :intake @17:28:16.29 Loading lyfil puck from tube 7 into Collection Clamp @17:28:16.52 SC.reconfigure :tight @17:28:16.57 SC.seek 7 @17:28:20.11 SC.configure :limp @17:28:20.32 Elbow.reconfigure :tight @17:28:20.37 Elbow.seek 7 @17:28:22.30 Forearm.reconfigure :tight @17:28:22.34 Forearm.seek 7 + 450 counts @17:28:23.41 SE.reconfigure :pick @17:28:23.47 SE.seek :beamBreak @17:28:25.05 SE.configure :default @17:28:25.22 Elbow.seek 7 @17:28:25.40 Forearm.seek 7 + 300 counts @17:28:25.60 Forearm.reconfigure :loose @17:28:25.65 Hand.close @17:28:26.27 SE.seek :down @17:28:27.03
Forearm.seek :retract @17:28:27.96 Elbow.seek CC @17:28:28.09 SE.stop @17:28:29.52
Elbow.reconfigure :loose @17:28:29.62 Forearm.reconfigure :tight @17:28:29.67 Forearm.seek CC @17:28:30.98 CC.seek :closed @17:28:35.51 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.20 @17:28:35.52 Hand.open @17:28:36.19 Forearm.reconfigure :loose @17:28:36.24 Forearm.seek :retract @17:28:37.23 CTV.dial CSV,avoiding: :intake @17:28:38.59 CSV.dial CTV @17:28:38.92 PS.seek 2.5ml @17:29:05.32 CS.seek 4.499ml @17:29:20.23 delay 8 seconds @17:29:28.25 PSV.dial :air @17:29:28.72 delay 2 seconds @17:29:30.73 PSV.dial :waste2 @17:29:31.49 PS.seek 0ml @17:29:57.89 CBV.dial between PRV and waste1 @17:29:58.21 CSV.dial :air @17:29:58.69 CS.seek 10ml @17:30:13.66 CSV.dial CTV @17:30:14.12 CS.seek 0ml @17:30:40.57 CBV.dial :waste1 @17:30:40.88 delay 5 seconds @17:30:45.90 CTV.dial :air,avoiding: :intake @17:30:47.75 Storing lyfil puck in Collection Clamp to tube 6 @17:30:47.93 SC.reconfigure :tight @17:30:47.98 SC.seek 6 @17:30:51.54 SC.configure :limp @17:30:51.75 Elbow.reconfigure :tight @17:30:51.80 Elbow.seek 6 @17:30:53.67 Forearm.reconfigure :tight @17:30:53.71 Forearm.seek 6 + 450 counts @17:30:54.86 SE.reconfigure :pick @17:30:54.92 SE.seek :beamBreak @17:30:57.61 SE.configure :default @17:30:57.77 Elbow.reconfigure :limp @17:30:57.82 Forearm.reconfigure :loose @17:30:57.86 Forearm.seek 6 @17:30:58.17 Elbow.hold @17:30:58.24 Elbow.reconfigure :tight @17:30:58.34 SE.seek 7.16 Pucks below Top Plate @17:30:58.83 Forearm.force -20 @17:30:58.87 Forearm.seek 6 + 298 counts @17:30:59.17 Forearm.reconfigure :tight @17:30:59.22 Forearm.seek 6 + 450 counts @17:30:59.41 SE.seek 8.16 Pucks below Top Plate @17:31:00.01 Forearm.reconfigure :loose @17:31:00.06 Forearm.seek :retract @17:31:00.99 Elbow.seek CC @17:31:02.68 Elbow.reconfigure :loose @17:31:02.78 Forearm.seek CC @17:31:03.77 Hand.close @17:31:04.77 CC.seek :open @17:31:09.52 Forearm.seek :retract @17:31:10.51 Elbow.reconfigure :tight @17:31:10.55 Elbow.seek 6 @17:31:12.32 Elbow.reconfigure :limp @17:31:12.38 Forearm.seek 6 @17:31:13.41 Elbow.hold @17:31:13.48 Elbow.reconfigure :tight @17:31:13.52 Hand.open @17:31:14.26 Forearm.force -20 @17:31:14.31 Forearm.seek 6 + 308 counts @17:31:14.59 Forearm.reconfigure :tight @17:31:14.64 Forearm.seek 6 + 450 counts @17:31:14.88 SE.seek :down @17:31:16.02
Forearm.reconfigure :loose @17:31:16.06 Forearm.seek :retract @17:31:17.03 SE.stop @17:31:17.07
PSV.dial :air @17:31:17.81 PS.seek 2.5ml @17:31:44.14 delay 1 second @17:31:45.15 PSV.dial :mixloop @17:31:45.61 delay 1 second @17:31:46.62 PSV.dial :air @17:31:47.09 delay 1 second @17:31:48.10 PSV.dial :mixloop @17:31:48.57 delay 1 second @17:31:49.59 PSV.dial :air @17:31:50.06 delay 1 second @17:31:51.08 PS.seek 0ml @17:32:17.45 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @17:32:17.63 Elbow.seek FlushPuck::Garage @17:32:19.46 Elbow.reconfigure :loose @17:32:19.57 Forearm.seek :garage @17:32:20.56 Hand.close @17:32:21.25 Forearm.seek :retract @17:32:22.22 Elbow.reconfigure :tight @17:32:22.27 Elbow.seek PC @17:32:23.78 Elbow.reconfigure :loose @17:32:23.88 Forearm.reconfigure :tight @17:32:23.93 Forearm.seek PC @17:32:25.13 PC.seek :closed @17:32:29.77 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.63 @17:32:29.83 Hand.open @17:32:30.56 Forearm.reconfigure :loose @17:32:30.61 Forearm.seek :retract @17:32:31.63 PRV.dial :air @17:32:32.10 PSV.dial :mixloop @17:32:32.63 PS.seek 0.3ml @17:32:36.48 PSV.dial PSR @17:32:37.54 Pulling 0.4ml of PSR.air @17:32:37.55 PSR.select :air @17:32:37.57 PS.seek 0.7ml @17:32:42.51 delay 3 seconds @17:32:45.53 PSR.select :air @17:32:45.55 PS.seek 2.5ml @17:33:04.82 delay 3 seconds @17:33:07.84 PSV.dial :mixloop @17:33:08.90 PTV.dial :mixing @17:33:09.67 PBV.dial :waste2 @17:33:10.62 PS.seek 0ml @17:33:37.58 Garaging FlushPuck in Processing Clamp @17:33:37.65 Elbow.reconfigure :tight @17:33:37.70 Elbow.seek PC @17:33:38.04 Elbow.reconfigure :loose @17:33:38.14 Forearm.seek PC @17:33:39.08 Hand.close @17:33:39.76 PC.seek :open @17:33:44.47 Forearm.seek :retract @17:33:45.47 Elbow.reconfigure :tight @17:33:45.52 Elbow.seek :garage @17:33:46.86 Elbow.reconfigure :loose @17:33:46.95 Forearm.reconfigure :tight @17:33:47.00 Forearm.seek :garage @17:33:48.09 Hand.open @17:33:48.81 Forearm.reconfigure :loose @17:33:48.86 Forearm.seek :retract @17:33:49.87 Loading da puck from tube 7 into Processing Clamp @17:33:50.10 SC.reconfigure :tight @17:33:50.15 SC.seek 7 @17:33:53.68 SC.configure :limp @17:33:53.89 Elbow.reconfigure :tight @17:33:53.94 Elbow.seek 7 @17:33:55.84 Forearm.reconfigure :tight @17:33:55.89 Forearm.seek 7 + 450 counts @17:33:56.95 SE.reconfigure :pick @17:33:57.01 SE.seek :beamBreak @17:33:58.75 SE.configure :default @17:33:58.91 Elbow.seek 7 @17:33:59.10 Forearm.seek 7 + 300 counts @17:33:59.61 Forearm.reconfigure :loose @17:33:59.65 Hand.close @17:34:00.27 SE.seek :down @17:34:00.46
Forearm.seek :retract @17:34:01.41 Elbow.seek PC @17:34:01.63 SE.stop @17:34:03.96
Elbow.reconfigure :loose @17:34:04.07 Forearm.reconfigure :tight @17:34:04.11 Forearm.seek PC @17:34:05.31 PC.seek :closed @17:34:09.98 Processing Clamp onSpring/offSpring for 16/26 samples, on/off torque 1.65 @17:34:09.99 Hand.open @17:34:10.68 Forearm.reconfigure :loose @17:34:10.73 Forearm.seek :retract @17:34:11.67 Heating Puck.from 7, :da in Processing Clamp to 28C for 2:00:00 @17:34:11.69 PH.seek 28C,5:00 @17:34:53.34 PH.hold 2:00:00,28C @17:34:53.39 delay 1:30 @17:36:23.43 PSR.select :air @17:36:23.44 PSV.dial PSR @17:36:24.51 Pulling 0.5ml of PSR.wash @17:36:24.52 PSR.select :wash @17:36:24.64 PS.seek 0.5ml @17:36:30.60 delay 5 seconds @17:36:35.62 PSR.select :air @17:36:35.74 PS.seek 2ml @17:36:51.94 PTV.dial :puck @17:36:52.69 PSV.dial PTV @17:36:53.46 PBV.dial :waste1 @17:36:54.86 PS.seek 1.5ml @17:37:00.82 delay 3 seconds @17:37:03.83 PBV.dial between PSV and waste1 @17:37:04.20 PS.seek 0ml @17:37:20.35 delay 5:00 @17:42:20.38 recoverPSRreagent08 @17:42:20.39 PTV.dial :puck @17:42:20.58 PBV.dial :waste1 @17:42:20.95 delay 30 seconds @17:42:50.98 PBV.dial between PSV and waste1 @17:42:51.36 PSR.select :air @17:42:51.37 PSV.dial PSR @17:42:52.14 PS.reconfigure :fast @17:42:52.19 PS.seek 2.5ml @17:43:16.15 PSV.dial PTV @17:43:17.04 delay 5 seconds @17:43:22.06 PSV.dial PSR @17:43:22.81 delay 5 seconds @17:43:27.83 PSV.dial PTV @17:43:28.61 delay 5 seconds @17:43:33.63 PSV.dial :air @17:43:34.39 PS.seek 0ml @17:43:57.90 PTV.dial :PRVmixing,via: PRV @17:43:59.68 PSV.dial :mixloop @17:44:00.17 PRV.dial :ab1 @17:44:00.94 PS.reconfigure :slow @17:44:00.99 PS.seek 0.15ml @17:44:04.50 delay 2 seconds @17:44:06.52 CBV.dial PRV @17:44:06.54
PRV.dial CBV @17:44:07.13 PS.seek 0.35ml @17:44:11.65 delay 2 seconds @17:44:13.67 CBV.dial between PRV and PSV @17:44:13.98 PRV.dial :air @17:44:14.46 PS.seek 0.9ml @17:44:26.18 PRV.dial :waste2 @17:44:26.94 PS.seek 0.5ml @17:44:35.56 addPMLReagent08 @17:44:35.57 PSV.dial :air @17:44:36.05 PS.reconfigure :default @17:44:36.09 PS.seek 2ml @17:44:52.24 PSV.dial :PML @17:44:52.71 PTV.dial :mixing @17:44:53.48 PBV.dial :waste1 @17:44:53.92 PS.seek 1.85ml @17:44:56.31 PBV.dial between PSV and waste1 @17:44:56.69 PS.seek 0ml @17:45:16.40 delay 2 seconds @17:45:18.42 Beginning samplePassC @17:45:18.44 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @17:45:18.63 Elbow.reconfigure :tight @17:45:18.67 Elbow.seek FlushPuck::Garage @17:45:20.05 Elbow.reconfigure :loose @17:45:20.15 Forearm.seek :garage @17:45:21.12 Hand.close @17:45:21.86 Forearm.seek :retract @17:45:22.85 Elbow.reconfigure :tight @17:45:22.90 Elbow.seek CC @17:45:25.78 Elbow.reconfigure :loose @17:45:25.88 Forearm.reconfigure :tight @17:45:25.93 Forearm.seek CC @17:45:27.61 CC.seek :closed @17:45:32.13 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.48 @17:45:32.20 Hand.open @17:45:32.93 Forearm.reconfigure :loose @17:45:32.97 Forearm.seek :retract @17:45:33.93 PSV.dial PSR @17:45:35.01 Pulling 1.5ml of PSR.flush @17:45:35.02 PSR.select :flush @17:45:35.14 PS.seek 1.5ml @17:45:51.49 delay 5 seconds @17:45:56.51 PSV.dial :waste1 @17:45:56.98 PS.seek 0ml @17:46:13.09 PSV.dial PSR @17:46:13.56 PSR.select :air @17:46:13.67 PS.seek 2.5ml @17:46:40.00 PRV.dial CBV,avoiding: :ab2 @17:46:40.02
CBV.dial PRV @17:46:41.66 PSV.dial :mixloop @17:46:42.72 CTV.dial :air,avoiding: :intake @17:46:42.79 PTV.dial :PRVmixing @17:46:43.56 PS.seek 0ml @17:47:10.08 PSV.dial :air @17:47:10.56 PS.seek 2.5ml @17:47:36.83 PSV.dial :mixloop @17:47:37.33 PS.seek 0ml @17:48:03.74 PSV.dial :air @17:48:04.34 PS.seek 2.5ml @17:48:30.61 PSV.dial :mixloop @17:48:31.08 PS.seek 0ml @17:48:57.40 Garaging FlushPuck in Collection Clamp @17:48:57.47 Elbow.reconfigure :tight @17:48:57.90 Elbow.seek CC @17:48:58.28 Elbow.reconfigure :loose @17:48:58.38 Forearm.seek CC @17:48:59.41 Hand.close @17:48:59.99 CC.seek :open @17:49:04.60 Forearm.seek :retract @17:49:05.60 Elbow.reconfigure :tight @17:49:05.65 Elbow.seek :garage @17:49:08.56 Elbow.reconfigure :loose @17:49:08.66 Forearm.reconfigure :tight @17:49:08.71 Forearm.seek :garage @17:49:09.74 Hand.open @17:49:10.42 Forearm.reconfigure :loose @17:49:10.47 Forearm.seek :retract @17:49:11.46 delayUntil "17:53:18.41" @17:49:11.49 Whole Cell Archive sampling 1000ml @17:49:11.50 Loading wcr puck from tube 7 into Collection Clamp @17:49:11.98 Elbow.reconfigure :tight @17:49:12.03 Elbow.seek 7 @17:49:14.00 Forearm.reconfigure :tight @17:49:14.06 Forearm.seek 7 + 450 counts @17:49:15.09 SE.reconfigure :pick @17:49:15.15 SE.seek :beamBreak @17:49:16.89 SE.configure :default @17:49:17.06 Elbow.seek 7 @17:49:17.25 Forearm.seek 7 + 300 counts @17:49:17.89 Forearm.reconfigure :loose @17:49:17.94 Hand.close @17:49:18.63 SE.seek :down @17:49:18.84 Forearm.seek :retract @17:49:19.79 Elbow.seek CC @17:49:20.07 SE.stop @17:49:21.42 Elbow.reconfigure :loose @17:49:21.53 Forearm.reconfigure :tight @17:49:21.58 Forearm.seek CC @17:49:22.86 CC.seek :closed @17:49:27.41 Collection Clamp onSpring/offSpring for 14/27 samples, on/off torque 2.29 @17:49:27.42 Hand.open @17:49:28.05 Forearm.reconfigure :loose @17:49:28.10 Forearm.seek :retract @17:49:29.07 collectSample 1000ml w/bubble point of 35psi @17:49:29.08 Priming 7ml @17:49:29.09 Intake.select :close @17:49:29.11 Exhaust.select :close @17:49:29.12 SS.hold @17:49:29.33 Calibrating pressure sensors @17:49:29.34 CTV.dial :air @17:49:29.41 SSV.dial :spit,avoiding: :exhaust @17:49:29.51 SS.reconfigure :fast @17:49:29.56 SS.seek 5ml @17:49:32.28 SSV.dial CBV @17:49:32.30 CBV.dial SSV @17:49:33.70 delay 3.8 seconds @17:49:38.30 Sensor limits: Intake max 99.9psia; Outlet max 92.3psia @17:49:38.31 SSV.dial :spit @17:49:39.68 SS.seek 0ml @17:49:42.85 SSV.dial CBV @17:49:44.20 CTV.dial :intake @17:49:44.98 SSV.dial CBV @17:49:45.00 CBV.dial SSV @17:49:45.15 Allowing 40s for 7.0ml of remaining 7.0ml @17:49:45.16 Intake.select :open @17:49:47.33 Intake @14.5psia, vacuum from 9.0 to 12.0psi @17:49:47.35 SS.reconfigure :inhaling 2.20ml/s @17:49:47.40 SS.seek 7ml @17:49:53.14 Stroke completed -- waiting for pressure to equalize ... @17:49:53.15 SS.reconfigure :exhale @17:49:53.20 SS.hold @17:50:15.67 Intake.select :close @17:50:15.88 SSV.dial :exhaust @17:50:16.64 Exhaust.select :open @17:50:16.76 SS.seek 0ml @17:50:20.89 Exhaust.select :close @17:50:21.01 Sampling 1000.0ml @17:50:21.02 Intake.select :close @17:50:21.03 Exhaust.select :close @17:50:21.04 SS.reconfigure :default @17:50:21.09 SS.hold @17:50:21.30 CTV.dial :intake @17:50:21.38 SSV.dial CBV @17:50:21.40 CBV.dial SSV @17:50:22.20 Allowing 130s for 25.0ml of remaining 1000.0ml @17:50:22.22 Intake.select :open @17:50:24.38 Intake @15.1psia, vacuum from 9.6 to 12.6psi @17:50:24.40 SS.reconfigure :inhaling 2.20ml/s @17:50:24.45 SS.seek 25ml @17:50:32.41 Vacuum too high -- waiting up to 40 seconds @17:50:32.43 SS.reconfigure :exhale @17:50:32.48 SS.hold @17:50:41.42 Intake @18.3psia, vacuum from 10.0 to 13.0psi @17:50:41.43 SS.reconfigure :inhaling 1.76ml/s @17:50:41.48 SS.seek 25ml @17:50:44.69 Vacuum too high -- waiting up to 40 seconds @17:50:44.71 SS.reconfigure :exhale @17:50:44.77 SS.hold @17:50:47.01 Intake @17.4psia, vacuum from 10.0 to 13.0psi @17:50:47.03 SS.reconfigure :inhaling 1.41ml/s @17:50:47.08 SS.seek 25ml @17:50:51.82 Vacuum too high -- waiting up to 40 seconds @17:50:51.83 SS.reconfigure :exhale @17:50:51.89 SS.hold @17:50:56.26 Intake @18.7psia, vacuum from 10.0 to 13.0psi @17:50:56.28 SS.reconfigure :inhaling 1.13ml/s @17:50:56.78 SS.seek 25ml @17:51:01.19 Vacuum too high -- waiting up to 40 seconds @17:51:01.21 SS.reconfigure :exhale @17:51:01.27 SS.hold @17:51:03.41 Intake @18.1psia, vacuum from 10.0 to 13.0psi @17:51:03.42 SS.reconfigure :inhaling 0.91ml/s @17:51:03.48 SS.seek 25ml @17:51:05.09 Stroke completed -- waiting for pressure to equalize ... @17:51:05.10 SS.reconfigure :exhale @17:51:05.15 SS.hold @17:51:32.17 Intake.select :close @17:51:32.32 SSV.dial :exhaust @17:51:33.09 Exhaust.select :open @17:51:33.21 SS.seek 0ml @17:51:42.39 Exhaust.select :close @17:51:42.49 SSV.dial CBV @17:51:42.54 CBV.dial SSV @17:51:43.28 Allowing 130s for 25.0ml of remaining 975.0ml @17:51:43.29 Intake.select :open @17:51:45.47 Intake @15.7psia, vacuum from 10.0 to 13.0psi @17:51:45.48 SS.reconfigure :inhaling 0.91ml/s @17:51:45.53 SS.seek 25ml @17:52:15.45 Stroke completed -- waiting for pressure to equalize ... @17:52:15.46 SS.reconfigure :exhale @17:52:15.51 SS.hold @17:52:48.31 Intake.select :close @17:52:48.47 SSV.dial :exhaust @17:52:49.25 Exhaust.select :open @17:52:49.37 SS.seek 0ml @17:52:58.45 Exhaust.select :close @17:52:58.56 SSV.dial CBV @17:52:58.58 CBV.dial SSV @17:52:59.35 Allowing 130s for 25.0ml of remaining 950.0ml @17:52:59.37 Intake.select :open @17:53:01.54 Intake @15.8psia, vacuum from 10.0 to 13.0psi @17:53:01.56 SS.reconfigure :inhaling 0.91ml/s @17:53:01.60 SS.seek 25ml @17:53:18.42 recoverPRVreagent08 @17:53:18.43 PTV.dial :mixing @17:53:19.19 PBV.dial :waste1 @17:53:19.56 delay 30 seconds @17:53:31.48 Stroke completed -- waiting for pressure to equalize ... @17:53:31.49 SS.reconfigure :exhale @17:53:31.92 SS.hold @17:53:49.58 PBV.dial between PSV and waste1 @17:53:49.95 PSV.dial :air @17:53:50.43 PS.reconfigure :fast @17:53:50.48 PS.seek 2.5ml @17:54:00.60 Intake.select :close @17:54:00.76 SSV.dial :exhaust @17:54:01.52 Exhaust.select :open @17:54:01.64 SS.seek 0ml @17:54:10.46 Exhaust.select :close @17:54:10.58 SSV.dial CBV @17:54:10.60 CBV.dial SSV @17:54:11.37 Allowing 130s for 25.0ml of remaining 925.0ml @17:54:11.38 Intake.select :open @17:54:13.55 Intake @15.8psia, vacuum from 10.0 to 13.0psi @17:54:13.57 SS.reconfigure :inhaling 0.91ml/s @17:54:13.62 SS.seek 25ml @17:54:14.51 PSV.dial :mixloop @17:54:15.00 delay 2 seconds @17:54:17.02 PSV.dial :air @17:54:17.49 delay 2 seconds @17:54:19.50 PSV.dial :mixloop @17:54:19.99 delay 2 seconds @17:54:22.01 PSV.dial :air @17:54:22.60 PS.seek 0ml @17:54:44.02 Stroke completed -- waiting for pressure to equalize ... @17:54:44.03 SS.reconfigure :exhale @17:54:44.08 SS.hold @17:54:46.14 washCycleDA #1 of 2 @17:54:46.15 PSR.select :air @17:54:46.17 PSV.dial PSR @17:54:47.51 Pulling 0.5ml of PSR.wash @17:54:47.52 PSR.select :wash @17:54:47.71 PS.reconfigure :default @17:54:47.76 PS.seek 0.5ml @17:54:53.83 delay 5 seconds @17:54:58.84 PSR.select :air @17:54:58.96 PS.seek 2ml @17:55:12.78 Intake.select :close @17:55:12.94 SSV.dial :exhaust @17:55:13.71 Exhaust.select :open @17:55:13.83 SS.seek 0ml @17:55:15.14 PTV.dial :puck @17:55:15.89 PSV.dial PTV @17:55:16.67 PBV.dial :waste1 @17:55:17.12 PS.seek 1.5ml @17:55:22.87 Exhaust.select :close @17:55:22.98 SSV.dial CBV @17:55:23.00 CBV.dial SSV @17:55:23.11 delay 3 seconds @17:55:23.81 Allowing 130s for 25.0ml of remaining 900.0ml @17:55:23.82 Intake.select :open @17:55:25.99 Intake @15.8psia, vacuum from 10.0 to 13.0psi @17:55:26.01 SS.reconfigure :inhaling 0.91ml/s @17:55:26.06 SS.seek 25ml @17:55:26.12 PBV.dial between PSV and waste1 @17:55:26.52 PS.seek 0ml @17:55:42.67 delay 2:00 @17:55:55.92 Stroke completed -- waiting for pressure to equalize ... @17:55:55.93 SS.reconfigure :exhale @17:55:55.98 SS.hold @17:56:24.97 Intake.select :close @17:56:25.20 SSV.dial :exhaust @17:56:25.99 Exhaust.select :open @17:56:26.11 SS.seek 0ml @17:56:34.91 Exhaust.select :close @17:56:35.03 SSV.dial CBV @17:56:35.05 CBV.dial SSV @17:56:35.88 Allowing 130s for 25.0ml of remaining 875.0ml @17:56:35.90 Intake.select :open @17:56:38.06 Intake @15.8psia, vacuum from 10.0 to 13.0psi @17:56:38.08 SS.reconfigure :inhaling 0.91ml/s @17:56:38.13 SS.seek 25ml @17:57:05.12 Vacuum too high -- waiting up to 40 seconds @17:57:05.14 SS.reconfigure :exhale @17:57:05.20 SS.hold @17:57:16.47 Intake @17.7psia, vacuum from 10.0 to 13.0psi @17:57:16.49 SS.reconfigure :inhaling 0.73ml/s @17:57:16.54 SS.seek 25ml @17:57:20.54 Stroke completed -- waiting for pressure to equalize ... @17:57:20.55 SS.reconfigure :exhale @17:57:20.60 SS.hold @17:57:36.95 Intake.select :close @17:57:37.11 SSV.dial :exhaust @17:57:37.88 Exhaust.select :open @17:57:38.00 SS.seek 0ml @17:57:42.69 recoverPSRreagent08 @17:57:42.70 PTV.dial :puck @17:57:42.71 PBV.dial :waste1 @17:57:43.09 delay 30 seconds @17:57:46.86 Exhaust.select :close @17:57:46.97 SSV.dial CBV @17:57:47.01 CBV.dial SSV @17:57:47.78 Allowing 130s for 25.0ml of remaining 850.0ml @17:57:48.22 Intake.select :open @17:57:50.42 Intake @15.9psia, vacuum from 10.0 to 13.0psi @17:57:50.44 SS.reconfigure :inhaling 0.73ml/s @17:57:50.49 SS.seek 25ml @17:58:13.12 PBV.dial between PSV and waste1 @17:58:13.49 PSR.select :air @17:58:13.51 PSV.dial PSR @17:58:14.30 PS.reconfigure :fast @17:58:14.35 PS.seek 2.5ml @17:58:26.12 Vacuum too high -- waiting up to 40 seconds @17:58:26.14 SS.reconfigure :exhale @17:58:26.19 SS.hold @17:58:36.10 Intake @16.4psia, vacuum from 10.0 to 13.0psi @17:58:36.11 SS.reconfigure :inhaling 0.59ml/s @17:58:36.23 SS.seek 25ml @17:58:37.49 Stroke completed -- waiting for pressure to equalize ... @17:58:37.50 SS.reconfigure :exhale @17:58:37.54 SS.hold @17:58:38.89 PSV.dial PTV @17:58:39.73 delay 5 seconds @17:58:44.75 PSV.dial PSR @17:58:45.53 delay 5 seconds @17:58:50.55 PSV.dial PTV @17:58:51.34 delay 5 seconds @17:58:52.42 Intake.select :close @17:58:52.58 SSV.dial :exhaust @17:58:53.35 Exhaust.select :open @17:58:53.47 SS.seek 0ml @17:58:56.36 PSV.dial :air @17:58:57.13 PS.seek 0ml @17:59:02.28 Exhaust.select :close @17:59:02.39 SSV.dial CBV @17:59:02.41 CBV.dial SSV @17:59:03.21 Allowing 130s for 25.0ml of remaining 825.0ml @17:59:03.22 Intake.select :open @17:59:05.39 Intake @15.9psia, vacuum from 10.0 to 13.0psi @17:59:05.41 SS.reconfigure :inhaling 0.59ml/s @17:59:05.46 SS.seek 25ml @17:59:20.89 washCycleDA #2 of 2 @17:59:20.90 PSR.select :air @17:59:20.92 PSV.dial PSR @17:59:22.28 Pulling 0.5ml of PSR.wash @17:59:22.29 PSR.select :wash @17:59:22.41 PS.reconfigure :default @17:59:22.45 PS.seek 0.5ml @17:59:28.42 delay 5 seconds @17:59:33.44 PSR.select :air @17:59:33.56 PS.seek 2ml @17:59:49.66 PTV.dial :puck @17:59:49.68 PSV.dial PTV @17:59:50.45 PBV.dial :waste1 @17:59:50.73 Stroke completed -- waiting for pressure to equalize ... @17:59:50.75 SS.reconfigure :exhale @17:59:50.80 SS.hold @17:59:50.97 PS.seek 1.5ml @17:59:56.88 delay 3 seconds @17:59:59.90 PBV.dial between PSV and waste1 @18:00:00.28 PS.seek 0ml @18:00:15.31 Intake.select :close @18:00:15.47 SSV.dial :exhaust @18:00:16.24 Exhaust.select :open @18:00:16.35 SS.seek 0ml @18:00:16.48 delay 2:00 @18:00:25.22 Exhaust.select :close @18:00:25.33 SSV.dial CBV @18:00:25.37 CBV.dial SSV @18:00:26.16 Allowing 130s for 25.0ml of remaining 800.0ml @18:00:26.17 Intake.select :open @18:00:28.34 Intake @15.9psia, vacuum from 10.0 to 13.0psi @18:00:28.36 SS.reconfigure :inhaling 0.59ml/s @18:00:28.41 SS.seek 25ml @18:01:01.81 Vacuum too high -- waiting up to 40 seconds @18:01:01.82 SS.reconfigure :exhale @18:01:01.87 SS.hold @18:01:11.64 Intake @16.2psia, vacuum from 10.0 to 13.0psi @18:01:11.66 SS.reconfigure :inhaling 0.47ml/s @18:01:11.71 SS.seek 25ml @18:01:26.04 Stroke completed -- waiting for pressure to equalize ... @18:01:26.06 SS.reconfigure :exhale @18:01:26.11 SS.hold @18:01:47.01 Intake.select :close @18:01:47.17 SSV.dial :exhaust @18:01:47.94 Exhaust.select :open @18:01:48.06 SS.seek 0ml @18:01:56.86 Exhaust.select :close @18:01:56.97 SSV.dial CBV @18:01:57.00 CBV.dial SSV @18:01:57.78 Allowing 130s for 25.0ml of remaining 775.0ml @18:01:57.80 Intake.select :open @18:01:59.96 Intake @15.7psia, vacuum from 10.0 to 13.0psi @18:01:59.98 SS.reconfigure :inhaling 0.47ml/s @18:02:00.03 SS.seek 25ml @18:02:16.50 recoverPSRreagent08 @18:02:16.51 PTV.dial :puck @18:02:16.53 PBV.dial :waste1 @18:02:16.90 delay 30 seconds @18:02:46.92 PBV.dial between PSV and waste1 @18:02:47.34 PSR.select :air @18:02:47.36 PSV.dial PSR @18:02:48.14 PS.reconfigure :fast @18:02:48.19 PS.seek 2.5ml @18:02:55.57 Stroke completed -- waiting for pressure to equalize ... @18:02:55.59 SS.reconfigure :exhale @18:02:55.64 SS.hold @18:03:12.13 PSV.dial PTV @18:03:12.90 delay 5 seconds @18:03:17.91 PSV.dial PSR @18:03:18.68 delay 5 seconds @18:03:23.69 PSV.dial PTV @18:03:24.58 delay 5 seconds @18:03:28.36 Intake.select :close @18:03:28.51 SSV.dial :exhaust @18:03:29.28 Exhaust.select :open @18:03:29.40 SS.seek 0ml @18:03:29.60 PSV.dial :air @18:03:30.39 PS.seek 0ml @18:03:38.18 Exhaust.select :close @18:03:38.29 SSV.dial CBV @18:03:38.32 CBV.dial SSV @18:03:39.09 Allowing 130s for 25.0ml of remaining 750.0ml @18:03:39.11 Intake.select :open @18:03:41.27 Intake @15.7psia, vacuum from 10.0 to 13.0psi @18:03:41.29 SS.reconfigure :inhaling 0.47ml/s @18:03:41.34 SS.seek 25ml @18:03:53.97 getPMLReagent08 @18:03:53.98 PRV.dial :air @18:03:54.44 delay 5 seconds @18:03:59.46 PTV.dial :PRVmixing @18:04:00.82 delay 5 seconds @18:04:05.84 PRV.dial :ab2 @18:04:06.30 PTV.dial :PRVmixing @18:04:06.38 PSV.dial :mixloop @18:04:07.31 PS.reconfigure :default @18:04:07.36 PS.seek 0.3ml @18:04:11.22 delay 2 seconds @18:04:13.24 PRV.dial :air @18:04:13.78 PS.seek 0.55ml @18:04:17.10 addPMLReagent08 @18:04:17.11 PSV.dial :air @18:04:17.59 PS.seek 2ml @18:04:33.25 PSV.dial :PML @18:04:33.72 PTV.dial :mixing @18:04:34.28 Vacuum too high -- waiting up to 40 seconds @18:04:34.29 SS.reconfigure :exhale @18:04:34.35 SS.hold @18:04:34.50 PBV.dial :waste1 @18:04:35.02 PS.seek 1.85ml @18:04:37.42 PBV.dial between PSV and waste1 @18:04:37.81 PS.seek 0ml @18:04:57.47 delay 2 seconds @18:04:59.49 delay 8:00 @18:04:59.72 Intake @17.6psia, vacuum from 10.0 to 13.0psi @18:04:59.74 SS.reconfigure :inhaling 0.38ml/s @18:04:59.79 SS.seek 25ml @18:05:03.38 Stroke completed -- waiting for pressure to equalize ... @18:05:03.40 SS.reconfigure :exhale @18:05:03.44 SS.hold @18:05:21.83 Intake.select :close @18:05:21.99 SSV.dial :exhaust @18:05:22.78 Exhaust.select :open @18:05:22.90 SS.seek 0ml @18:05:32.57 Exhaust.select :close @18:05:32.69 SSV.dial CBV @18:05:32.71 CBV.dial SSV @18:05:33.48 Allowing 130s for 25.0ml of remaining 725.0ml @18:05:33.49 Intake.select :open @18:05:35.66 Intake @15.7psia, vacuum from 10.0 to 13.0psi @18:05:35.68 SS.reconfigure :inhaling 0.38ml/s @18:05:35.73 SS.seek 25ml @18:06:43.77 Stroke completed -- waiting for pressure to equalize ... @18:06:43.78 SS.reconfigure :exhale @18:06:43.83 SS.hold @18:07:24.02 SS.reconfigure :default @18:07:24.07 SS.hold @18:07:24.14 Intake.select :close @18:07:24.30 ShallowSampler::Clogged in WCR -- Intake @16psia & Outlet @13psia won't equalize after 300.0ml @18:07:24.31 SSV.dial :exhaust @18:07:25.07 Exhaust.select :open @18:07:25.18 SS.reconfigure :exhale @18:07:25.24 SS.seek 0ml @18:07:34.38 Exhaust.select :close @18:07:34.50 Sampled 300.0ml @18:07:34.51 Drying puck before fixing for 5:00 @18:07:34.52 CBV.dial :waste2b @18:07:35.28 delay 1:00 @18:08:35.31 CSV.dial :air @18:08:35.77 CS.reconfigure :fast @18:08:35.82 CS.seek 10ml @18:09:00.35 Pumping wcr puck from tube 7 up to 35psi... @18:09:00.36 CBV.dial SSV @18:09:01.63 CBV.dial between CSV and waste2b @18:09:02.62 CSV.dial between air and CTV @18:09:02.95 CS.seek 7.163ml @18:09:10.35 CTV.dial CSV,avoiding: :air @18:09:10.37 CSV.dial CTV @18:09:11.21 CS.reconfigure :pressurize @18:09:11.26 CS.seek 0ml @18:09:25.33 CBV.dial :waste2b @18:09:25.68 CS.reconfigure :default @18:09:25.73 CS.seek 0ml @18:09:31.19 delay 1:00 @18:10:31.22 CTV.dial :air,avoiding: :intake @18:10:32.54 Fix #1 of 2 (with RNAlater) for 2:30 @18:10:32.55 CSV.dial CSR @18:10:33.00 Pulling 2ml of CSR.RNAlater @18:10:33.01 CSR.select :RNAlater @18:10:33.13 CS.seek 2ml @18:10:39.12 delay 5 seconds @18:10:44.14 CSR.select :air @18:10:44.26 CS.reconfigure :fast @18:10:44.31 CS.seek 10ml @18:11:04.09 Pumping wcr puck from tube 7 up to 35psi... @18:11:04.11 CBV.dial SSV @18:11:05.36 CBV.dial between CSV and waste2b @18:11:06.34 CSV.dial between air and CTV @18:11:06.94 CS.seek 7.2ml @18:11:14.19 CTV.dial CSV,avoiding: :air @18:11:14.21 CSV.dial CTV @18:11:15.60 CS.reconfigure :pressurize @18:11:15.65 CS.seek 0ml @18:11:29.46 delay 2:30 @18:12:59.52 recoverPRVreagent08 @18:12:59.53 PTV.dial :mixing @18:12:59.71 PBV.dial :waste1 @18:13:00.09 delay 30 seconds @18:13:30.11 PBV.dial between PSV and waste1 @18:13:30.49 PSV.dial :air @18:13:30.97 PS.reconfigure :fast @18:13:31.02 PS.seek 2.5ml @18:13:54.99 PSV.dial :mixloop @18:13:55.46 delay 2 seconds @18:13:57.48 PSV.dial :air @18:13:58.01 delay 2 seconds @18:13:59.48 CBV.dial :waste2b @18:13:59.83 CS.reconfigure :default @18:13:59.88 CS.seek 0ml @18:14:00.02 PSV.dial :mixloop @18:14:00.49 delay 2 seconds @18:14:02.51 PSV.dial :air @18:14:02.98 PS.seek 0ml @18:14:05.69 delay 1:00 @18:14:26.46 washCycleDA #1 of 3 @18:14:26.47 PSR.select :air @18:14:26.49 PSV.dial PSR @18:14:27.85 Pulling 0.5ml of PSR.wash @18:14:27.86 PSR.select :wash @18:14:27.98 PS.reconfigure :default @18:14:28.03 PS.seek 0.5ml @18:14:33.99 delay 5 seconds @18:14:39.01 PSR.select :air @18:14:39.14 PS.seek 2ml @18:14:55.33 PTV.dial :puck @18:14:56.10 PSV.dial PTV @18:14:56.98 PBV.dial :waste1 @18:14:57.43 PS.seek 1.5ml @18:15:03.37 delay 3 seconds @18:15:05.72 CTV.dial :air,avoiding: :intake @18:15:06.38 PBV.dial between PSV and waste1 @18:15:06.76 PS.seek 0ml @18:15:07.05 Fix #2 of 2 (with RNAlater) for 2:30 @18:15:07.06 CSV.dial CSR @18:15:07.49 Pulling 2ml of CSR.RNAlater @18:15:07.51 CSR.select :RNAlater @18:15:07.63 CS.seek 2ml @18:15:13.53 delay 5 seconds @18:15:18.55 CSR.select :air @18:15:18.66 CS.reconfigure :fast @18:15:18.72 CS.seek 10ml @18:15:23.12 delay 2:00 @18:15:38.59 Pumping wcr puck from tube 7 up to 35psi... @18:15:38.61 CBV.dial SSV @18:15:39.86 CBV.dial between CSV and waste2b @18:15:40.84 CSV.dial between air and CTV @18:15:41.44 CS.seek 7.152ml @18:15:48.78 CTV.dial CSV,avoiding: :air @18:15:48.81 CSV.dial CTV @18:15:50.20 CS.reconfigure :pressurize @18:15:50.26 CS.seek 0ml @18:16:09.15 CSV.dial :air @18:16:09.69 CS.reconfigure :fast @18:16:09.75 CS.seek 10ml @18:16:34.37 CSV.dial between air and CTV @18:16:34.71 CS.seek 3.923ml @18:16:49.60 CTV.dial CSV,avoiding: :air @18:16:49.62 CSV.dial CTV @18:16:50.00 CS.reconfigure :pressurize @18:16:50.05 CS.seek 0ml @18:17:00.59 CSV.dial :air @18:17:01.05 CS.reconfigure :fast @18:17:01.10 CS.seek 10ml @18:17:23.14 recoverPSRreagent08 @18:17:23.16 PTV.dial :puck @18:17:23.22 PBV.dial :waste1 @18:17:23.60 delay 30 seconds @18:17:25.74 CSV.dial between air and CTV @18:17:26.06 CS.seek 4.365ml @18:17:39.94 CTV.dial CSV,avoiding: :air @18:17:39.96 CSV.dial CTV @18:17:40.39 CS.reconfigure :pressurize @18:17:40.44 CS.seek 0ml @18:17:52.12 CSV.dial :air @18:17:52.56 CS.reconfigure :fast @18:17:52.61 CS.seek 10ml @18:17:53.62 PBV.dial between PSV and waste1 @18:17:53.99 PSR.select :air @18:17:54.01 PSV.dial PSR @18:17:54.89 PS.reconfigure :fast @18:17:54.93 PS.seek 2.5ml @18:18:17.21 CSV.dial between air and CTV @18:18:17.53 CS.seek 4.444ml @18:18:18.77 PSV.dial PTV @18:18:19.52 delay 5 seconds @18:18:24.54 PSV.dial PSR @18:18:25.30 delay 5 seconds @18:18:30.32 PSV.dial PTV @18:18:31.08 delay 5 seconds @18:18:31.16 CTV.dial CSV,avoiding: :air @18:18:31.18 CSV.dial CTV @18:18:31.95 CS.reconfigure :pressurize @18:18:32.01 CS.seek 0ml @18:18:36.10 PSV.dial :air @18:18:36.87 PS.seek 0ml @18:18:43.87 CSV.dial :air @18:18:44.32 CS.reconfigure :default @18:18:44.37 CS.hold @18:18:44.49 ShallowSampler::Error in MAIN -- Puck.from 7, :wcr reached only 25.2 of 35psi after pumping in 40ml of gas @18:18:44.51 Waiting to Resume from Checkpoint "Pumping wcr puck from tube 7 up to 35psi..." @18:18:44.52 BadNews.email "Puck.from 7, :wcr reached only 25.2 of 35psi after pumping in 40ml of gas",Subject: "ShallowSampler::Error in MAIN" @18:18:49.33 upsync @18:19:00.35 washCycleDA #2 of 3 @18:19:00.37 PSR.select :air @18:19:00.44 PSV.dial PSR @18:19:01.80 Pulling 0.5ml of PSR.wash @18:19:01.81 PSR.select :wash @18:19:01.94 PS.reconfigure :default @18:19:02.00 PS.seek 0.5ml @18:19:07.90 delay 5 seconds @18:19:12.92 PSR.select :air @18:19:13.04 PS.seek 2ml @18:19:29.18 PTV.dial :puck @18:19:29.25 PSV.dial PTV @18:19:30.00 PBV.dial :waste1 @18:19:30.44 PS.seek 1.5ml @18:19:36.36 delay 3 seconds @18:19:39.38 PBV.dial between PSV and waste1 @18:19:39.76 PS.seek 0ml @18:19:55.93 delay 2:00 @18:21:55.96 recoverPSRreagent08 @18:21:55.97 PTV.dial :puck @18:21:56.05 PBV.dial :waste1 @18:21:56.42 delay 30 seconds @18:22:26.45 PBV.dial between PSV and waste1 @18:22:26.83 PSR.select :air @18:22:26.85 PSV.dial PSR @18:22:27.62 PS.reconfigure :fast @18:22:27.67 PS.seek 2.5ml @18:22:51.55 PSV.dial PTV @18:22:52.31 delay 5 seconds @18:22:57.33 PSV.dial PSR @18:22:58.09 delay 5 seconds @18:23:03.12 PSV.dial PTV @18:23:03.88 delay 5 seconds @18:23:08.90 PSV.dial :air @18:23:09.79 PS.seek 0ml @18:23:33.33 washCycleDA #3 of 3 @18:23:33.80 PSR.select :air @18:23:33.85 PSV.dial PSR @18:23:35.20 Pulling 0.5ml of PSR.wash @18:23:35.21 PSR.select :wash @18:23:35.33 PS.reconfigure :default @18:23:35.38 PS.seek 0.5ml @18:23:41.39 delay 5 seconds @18:23:46.41 PSR.select :air @18:23:46.53 PS.seek 2ml @18:24:02.67 PTV.dial :puck @18:24:02.75 PSV.dial PTV @18:24:03.53 PBV.dial :waste1 @18:24:03.98 PS.seek 1.5ml @18:24:09.92 delay 3 seconds @18:24:12.94 PBV.dial between PSV and waste1 @18:24:13.32 PS.seek 0ml @18:24:29.48 delay 2:00 @18:26:29.51 recoverPSRreagent08 @18:26:29.52 PTV.dial :puck @18:26:29.59 PBV.dial :waste1 @18:26:29.96 delay 30 seconds @18:26:59.99 PBV.dial between PSV and waste1 @18:27:00.37 PSR.select :air @18:27:00.39 PSV.dial PSR @18:27:01.28 PS.reconfigure :fast @18:27:01.33 PS.seek 2.5ml @18:27:25.23 PSV.dial PTV @18:27:25.98 delay 5 seconds @18:27:31.00 PSV.dial PSR @18:27:31.76 delay 5 seconds @18:27:36.78 PSV.dial PTV @18:27:37.57 delay 5 seconds @18:27:42.60 PSV.dial :air @18:27:43.36 PS.seek 0ml @18:28:06.66 Gate.power :camera,:ON @18:28:06.68 Mixing DA Substrates @18:28:06.69 PSR.select :air @18:28:06.70 PSV.dial PSR @18:28:08.12 Pulling 0.25ml of PSR.sub1 @18:28:08.15 PSR.select :sub1 @18:28:08.34 PS.reconfigure :default @18:28:08.45 PS.seek 0.25ml @18:28:11.91 delay 5 seconds @18:28:16.94 Pulling 0.25ml of PSR.sub2 @18:28:16.95 PSR.select :sub2 @18:28:17.07 PS.seek 0.5ml @18:28:20.48 delay 5 seconds @18:28:25.50 PSR.select :air @18:28:25.61 PS.seek 2ml @18:28:41.76 PTV.dial :puck @18:28:41.84 PSV.dial PTV @18:28:42.62 PBV.dial :waste1 @18:28:43.07 PS.seek 1.5ml @18:28:48.94 delay 3 seconds @18:28:51.96 PBV.dial between PSV and waste1 @18:28:52.34 PS.seek 0ml @18:29:08.69 delay 10 seconds @18:29:18.72 recoverPSRreagent08 @18:29:18.73 PTV.dial :puck @18:29:18.80 PBV.dial :waste1 @18:29:19.20 delay 30 seconds @18:29:49.22 PBV.dial between PSV and waste1 @18:29:49.60 PSR.select :air @18:29:49.62 PSV.dial PSR @18:29:50.82 PS.reconfigure :fast @18:29:50.94 PS.seek 2.5ml @18:30:15.10 PSV.dial PTV @18:30:15.88 delay 5 seconds @18:30:20.90 PSV.dial PSR @18:30:21.68 delay 5 seconds @18:30:26.70 PSV.dial PTV @18:30:27.49 delay 5 seconds @18:30:32.51 PSV.dial :air @18:30:33.29 PS.seek 0ml @18:30:57.45 Additional air clear @18:30:57.46 PSR.select :air @18:30:57.47 PSV.dial PSR @18:30:58.85 Pulling 2ml of PSR.air @18:30:58.86 PSR.select :air @18:30:58.88 PS.reconfigure :default @18:30:58.93 PS.seek 2ml @18:31:20.25 delay 5 seconds @18:31:25.28 PTV.dial :puck @18:31:25.36 PSV.dial PTV @18:31:26.25 PBV.dial :waste1 @18:31:26.71 PS.seek 1.5ml @18:31:32.60 delay 3 seconds @18:31:35.62 PBV.dial between PSV and waste1 @18:31:36.01 delay 1 second @18:31:37.03 PSV.dial :waste1 @18:31:38.11 PS.seek 0ml @18:31:54.34 Processing Puck Heater at 28C before imaging @18:31:54.35 PH.coast @18:31:54.47 Imaging da puck in Processing Clamp while storing it to tube 6 @18:31:54.64 SC.reconfigure :tight @18:31:54.69 SC.seek 6 @18:31:58.33 SC.configure :limp @18:31:58.54 Elbow.reconfigure :tight @18:31:58.59 Elbow.seek 6 @18:32:00.37 Forearm.reconfigure :tight @18:32:00.42 Forearm.seek 6 + 450 counts @18:32:01.48 SE.reconfigure :pick @18:32:01.54 SE.seek :beamBreak @18:32:04.09 SE.configure :default @18:32:04.25 Elbow.reconfigure :limp @18:32:04.30 Forearm.reconfigure :loose @18:32:04.35 Forearm.seek 6 @18:32:04.58 Elbow.hold @18:32:04.64 Elbow.reconfigure :tight @18:32:04.74 SE.seek 8.14 Pucks below Top Plate @18:32:05.72 Forearm.force -20 @18:32:05.76 Forearm.seek 6 + 307 counts @18:32:06.06 Forearm.reconfigure :tight @18:32:06.11 Forearm.seek 6 + 450 counts @18:32:06.30 SE.seek 9.14 Pucks below Top Plate @18:32:06.93 Forearm.reconfigure :loose @18:32:06.98 Forearm.seek :retract @18:32:07.91 Elbow.seek PC @18:32:10.39 Elbow.reconfigure :loose @18:32:10.49 Forearm.seek PC @18:32:11.45 Hand.close @18:32:12.21 PC.seek :open @18:32:17.02 Forearm.seek :retract @18:32:18.02 Elbow.reconfigure :tight @18:32:18.07 Elbow.seek 6 @18:32:20.73 Forearm.reconfigure :tight @18:32:20.78 Forearm.seek 6 + 450 counts @18:32:21.78 Hand.open @18:32:22.52 SE.seek 8.79 Pucks below Top Plate @18:32:22.81 SE.reconfigure :seal @18:32:22.86 SE.seek 7.82 Pucks below Top Plate @18:32:23.44 Lights, camera, ACTION! @18:32:23.47 PuckCamera: snap -bin=2,2 -auto /var/log/esp/da23dec1918h600ml.tif @18:32:42.92 Waiting >20 seconds for da to relinquish FlushPuck @18:32:45.23 SX HX9/16 (USB:31): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 18.466 seconds /var/log/esp/da23dec1918h600ml.tif: TIFF Upload Complete @18:32:45.25 PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da23dec1918h600ml15s.tif @18:33:00.87 SX HX9/16 (USB:31): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15 seconds /var/log/esp/midres/da23dec1918h600ml15s.tif: TIFF Upload Complete @18:33:00.89 PuckCamera: snap -bin=2,2 20.0 /var/log/esp/midres/da23dec1918h600ml20s.tif @18:33:21.51 SX HX9/16 (USB:31): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 20 seconds /var/log/esp/midres/da23dec1918h600ml20s.tif: TIFF Upload Complete @18:33:21.53 DAfans.email "18.466 sec auto-exposure",Subject: "da23dec1918h600ml images!" @18:33:21.56 Gate.power :camera,:OFF @18:33:21.91 Elbow.seek 6 @18:33:22.41 Forearm.seek 6 + 450 counts @18:33:22.55 SE.reconfigure :gently @18:33:22.61 SE.seek 8.54 Pucks below Top Plate @18:33:23.52 Elbow.reconfigure :limp @18:33:23.59 Forearm.reconfigure :loose @18:33:23.64 Forearm.seek 6 @18:33:23.89 Elbow.hold @18:33:23.96 Elbow.reconfigure :tight @18:33:24.04 SE.reconfigure :default @18:33:24.09 SE.seek 8.72 Pucks below Top Plate @18:33:24.51 Forearm.force -20 @18:33:24.56 Forearm.seek 6 + 303 counts @18:33:24.85 Forearm.reconfigure :tight @18:33:24.90 Forearm.seek 6 + 450 counts @18:33:25.36 SE.seek :down @18:33:26.67 Forearm.reconfigure :loose @18:33:26.78 Forearm.seek :retract @18:33:27.65 SE.stop @18:33:27.93 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @18:33:28.34 Elbow.seek FlushPuck::Garage @18:33:30.32 Elbow.reconfigure :loose @18:33:30.53 Forearm.seek :garage @18:33:31.66 Hand.close @18:33:32.63 Forearm.seek :retract @18:33:33.70 Elbow.reconfigure :tight @18:33:33.82 Elbow.seek PC @18:33:35.19 Elbow.reconfigure :loose @18:33:35.98 Forearm.reconfigure :tight @18:33:36.07 Forearm.seek PC @18:33:37.70 PC.seek :closed @18:33:42.50 Processing Clamp onSpring/offSpring for 16/26 samples, on/off torque 1.64 @18:33:42.64 Hand.open @18:33:43.53 Forearm.reconfigure :loose @18:33:43.62 Forearm.seek :retract @18:33:44.61 PSR.select :air @18:33:44.65 PSV.dial PSR @18:33:45.17 Pulling 0.5ml of PSR.flush @18:33:45.20 PSR.select :flush @18:33:45.39 PS.seek 0.5ml @18:33:51.46 delay 5 seconds @18:33:54.44 upsync @18:33:56.48 PSR.select :air @18:33:56.61 PS.seek 2.5ml @18:34:17.81 delay 1 second @18:34:18.83 PRV.dial :waste2,avoiding: :ab2 @18:34:20.74 PTV.dial :PRVmixing @18:34:22.06 PSV.dial :mixloop @18:34:23.10 PS.seek :empty @18:34:49.53 PSR.select :air @18:34:49.55 PSV.dial PSR @18:34:50.61 Pulling 0.5ml of PSR.flush @18:34:50.62 PSR.select :flush @18:34:50.74 PS.seek 0.5ml @18:34:56.75 delay 5 seconds @18:35:01.77 PSR.select :air @18:35:01.89 PS.seek 2.5ml @18:35:23.13 delay 1 second @18:35:24.14 PBV.dial :waste1 @18:35:24.52 PTV.dial :mixing @18:35:25.27 PSV.dial :mixloop @18:35:26.33 PS.seek :empty @18:35:52.84 PSV.dial PSR @18:35:53.89 PSR.select :flush @18:35:54.01 PS.seek 1ml @18:36:05.03 PSR.select :air @18:36:05.15 PS.seek 2.5ml @18:36:21.31 PSV.dial PTV @18:36:22.08 PTV.dial :puck @18:36:22.83 PBV.dial :waste1 @18:36:22.85 PS.seek 0ml @18:36:49.15 PSV.dial PSR @18:36:49.92 PSR.select :flush @18:36:50.04 PS.seek 0.5ml @18:36:56.03 PSR.select :air @18:36:56.14 PS.seek 2.5ml @18:37:17.40 PSV.dial PTV @18:37:18.62 PBV.dial PSV @18:37:19.19 PS.seek 0ml @18:37:45.53 PSV.dial PBV @18:37:46.01 PS.seek 2.5ml @18:38:12.40 PSV.dial :waste1 @18:38:13.16 PS.seek 0ml @18:38:39.53 PSV.dial PSR @18:38:40.00 PSR.select :air @18:38:40.02 PS.seek 2.5ml @18:39:06.40 PSV.dial PTV @18:39:07.15 PBV.dial PSV @18:39:07.24 PS.seek 0ml @18:39:33.56 PSV.dial PBV @18:39:34.03 PS.seek 2.5ml @18:40:00.43 PSV.dial :waste1 @18:40:01.19 PS.seek 0ml @18:40:27.62 PSV.dial PSR @18:40:28.09 PSR.select :flush @18:40:28.21 PS.seek 1ml @18:40:39.24 PSR.select :air @18:40:39.36 PS.seek 2.5ml @18:40:55.46 PSV.dial PTV @18:40:56.21 PTV.dial :puck @18:40:56.29 PBV.dial :waste1 @18:40:56.86 PS.seek 0ml @18:41:23.15 PSV.dial PSR @18:41:23.92 PSR.select :flush @18:41:24.04 PS.seek 0.5ml @18:41:30.02 PSR.select :air @18:41:30.14 PS.seek 2.5ml @18:41:51.40 PSV.dial PTV @18:41:52.17 PBV.dial PSV @18:41:52.73 PS.seek 0ml @18:42:19.27 PSV.dial PBV @18:42:19.75 PS.seek 2.5ml @18:42:46.02 PSV.dial :waste1 @18:42:46.78 PS.seek 0ml @18:43:13.12 PSV.dial PSR @18:43:13.58 PSR.select :air @18:43:13.61 PS.seek 2.5ml @18:43:40.02 PSV.dial PTV @18:43:40.77 PBV.dial PSV @18:43:40.97 PS.seek 0ml @18:44:07.77 PSV.dial PBV @18:44:08.25 PS.seek 2.5ml @18:44:34.65 PSV.dial :waste1 @18:44:35.40 PS.seek 0ml @18:45:01.71 Garaging FlushPuck in Processing Clamp @18:45:01.78 Elbow.reconfigure :tight @18:45:01.83 Elbow.seek PC @18:45:01.96 Elbow.reconfigure :loose @18:45:02.06 Forearm.seek PC @18:45:03.02 Hand.close @18:45:03.70 PC.seek :open @18:45:08.42 Forearm.seek :retract @18:45:09.40 Elbow.reconfigure :tight @18:45:09.45 Elbow.seek :garage @18:45:10.80 Elbow.reconfigure :loose @18:45:10.90 Forearm.reconfigure :tight @18:45:10.95 Forearm.seek :garage @18:45:12.02 Hand.open @18:45:12.75 Forearm.reconfigure :loose @18:45:12.80 Forearm.seek :retract @19:19:54.23 Session Begun @19:19:57.86 -> Flush FlushPuck is in FlushPuck::Garage @19:19:59.90 -> CC Collection Clamp contains wcr puck from tube 7 @19:20:04.45 -> PC Processing Clamp open @19:21:02.38 -> CC.unloadPuck 6, :WCR @19:21:02.47 Puck::Warning in dave -- Unloading Puck.from 7, :wcr to Storage Carousel @19:21:02.49 Storing WCR puck in Collection Clamp to tube 6 @19:21:02.92 Elbow.reconfigure :tight @19:21:03.00 Elbow.seek 6 @19:21:04.83 Forearm.reconfigure :tight @19:21:04.90 Forearm.seek 6 + 450 counts @19:21:05.91 SE.reconfigure :pick @19:21:05.99 SE.seek :beamBreak @19:21:08.51 SE.configure :default @19:21:08.69 Elbow.reconfigure :limp @19:21:08.76 Forearm.reconfigure :loose @19:21:08.82 Forearm.seek 6 @19:21:09.07 Elbow.hold @19:21:09.61 Elbow.reconfigure :tight @19:21:09.73 SE.seek 9.13 Pucks below Top Plate @19:21:10.20 Forearm.force -20 @19:21:10.26 Forearm.seek 6 + 307 counts @19:21:10.58 Forearm.reconfigure :tight @19:21:10.66 Forearm.seek 6 + 450 counts @19:21:10.87 SE.seek 10.13 Pucks below Top Plate @19:21:11.48 Forearm.reconfigure :loose @19:21:11.55 Forearm.seek :retract @19:21:12.47 Elbow.seek CC @19:21:14.13 Elbow.reconfigure :loose @19:21:14.25 Forearm.seek CC @19:21:15.24 Hand.close @19:21:15.92 CC.seek :open @19:21:20.66 Forearm.seek :retract @19:21:21.64 Elbow.reconfigure :tight @19:21:21.71 Elbow.seek 6 @19:21:23.50 Elbow.reconfigure :limp @19:21:23.57 Forearm.seek 6 @19:21:24.60 Elbow.hold @19:21:24.69 Elbow.reconfigure :tight @19:21:24.76 Hand.open @19:21:25.54 Forearm.force -20 @19:21:25.59 Forearm.seek 6 + 314 counts @19:21:25.89 Forearm.reconfigure :tight @19:21:25.97 Forearm.seek 6 + 450 counts @19:21:26.25 SE.seek :down @19:21:27.38 Forearm.reconfigure :loose @19:21:27.45 Forearm.seek :retract @19:21:28.28 SE.stop @19:21:34.28 -> CC Collection Clamp open @19:21:39.08 -> Puck.count @19:21:39.16 SE.seek :down @19:21:39.25 SE.stop @19:21:39.37 SC.reconfigure :tight @19:21:39.44 SC.seek 1 @19:21:55.55 SC.configure :limp @19:21:56.19 Elbow.seek 1 @19:21:56.40 Forearm.reconfigure :tight @19:21:56.48 Forearm.seek 1 + 450 counts @19:21:57.54 SE.reconfigure :place @19:21:57.63 SE.seek :beamBreak @19:21:58.78 SE.configure :default @19:21:58.91 SE.seek :down @19:21:59.41 SE.stop @19:22:00.03 Forearm.reconfigure :loose @19:22:00.10 Forearm.seek :retract @19:22:01.11 SC.reconfigure :tight @19:22:01.18 SC.seek 2 @19:22:04.75 SC.configure :limp @19:22:04.94 Elbow.seek 2 @19:22:05.15 Forearm.reconfigure :tight @19:22:05.23 Forearm.seek 2 + 450 counts @19:22:06.28 SE.reconfigure :place @19:22:06.36 SE.seek :beamBreak @19:22:07.51 SE.configure :default @19:22:07.65 SE.seek :down @19:22:08.10 SE.stop @19:22:08.77 Forearm.reconfigure :loose @19:22:08.85 Forearm.seek :retract @19:22:09.81 SC.reconfigure :tight @19:22:09.88 SC.seek 3 @19:22:13.41 SC.configure :limp @19:22:13.59 Elbow.seek 3 @19:22:14.11 Forearm.reconfigure :tight @19:22:14.18 Forearm.seek 3 + 450 counts @19:22:15.38 SE.reconfigure :place @19:22:15.46 SE.seek :beamBreak @19:22:16.55 SE.configure :default @19:22:16.68 SE.seek :down @19:22:17.13 SE.stop @19:22:17.81 Forearm.reconfigure :loose @19:22:18.34 Forearm.seek :retract @19:22:19.29 SC.reconfigure :tight @19:22:19.37 SC.seek 4 @19:22:22.96 SC.configure :limp @19:22:23.14 Elbow.seek 4 @19:22:23.58 Forearm.reconfigure :tight @19:22:23.66 Forearm.seek 4 + 450 counts @19:22:24.72 SE.reconfigure :place @19:22:24.80 SE.seek :beamBreak @19:22:25.95 SE.configure :default @19:22:26.08 SE.seek :down @19:22:26.61 SE.stop @19:22:27.21 Forearm.reconfigure :loose @19:22:27.28 Forearm.seek :retract @19:22:28.26 SC.reconfigure :tight @19:22:28.34 SC.seek 5 @19:22:31.88 SC.configure :limp @19:22:32.06 Elbow.seek 5 @19:22:32.39 Forearm.reconfigure :tight @19:22:32.47 Forearm.seek 5 + 450 counts @19:22:33.65 SE.reconfigure :place @19:22:33.74 SE.seek :beamBreak @19:22:34.88 SE.configure :default @19:22:35.01 SE.seek :down @19:22:35.49 SE.stop @19:22:36.14 Forearm.reconfigure :loose @19:22:36.21 Forearm.seek :retract @19:22:37.16 SC.reconfigure :tight @19:22:37.23 SC.seek 6 @19:22:40.75 SC.configure :limp @19:22:40.93 Elbow.seek 6 @19:22:41.14 Forearm.reconfigure :tight @19:22:41.22 Forearm.seek 6 + 450 counts @19:22:42.26 SE.reconfigure :place @19:22:42.34 SE.seek :beamBreak @19:22:45.05 SE.configure :default @19:22:45.65 SE.seek :down @19:22:46.79 Forearm.reconfigure :loose @19:22:46.86 Forearm.seek :retract @19:22:47.62 SE.stop @19:22:47.84 SC.reconfigure :tight @19:22:47.92 SC.seek 7 @19:22:51.44 SC.configure :limp @19:22:51.62 Elbow.seek 7 @19:22:52.19 Forearm.reconfigure :tight @19:22:52.26 Forearm.seek 7 + 450 counts @19:22:53.32 SE.reconfigure :place @19:22:53.40 SE.seek :beamBreak @19:22:55.46 SE.configure :default @19:22:55.60 SE.seek :down @19:22:56.73 Forearm.reconfigure :loose @19:22:56.81 Forearm.seek :retract @19:22:56.97 SE.stop {1 => 33, 2 => 33, 3 => 33, 4 => 33, 5 => 33, 6 => 11, 7 => 20} @19:25:09.62 -> abort! 'mission complete' @19:25:09.63 CmdServer::Client::Error in dave -- Aborting Mission: mission complete @19:25:09.68 BadNews.email "mission complete",Subject: "DA_WCR_121923 ABORTED!" # @19:25:14.37 upsync @19:25:14.46 Session Ended + ESPwhich DA_WCR_122023.rb + fn=DA_WCR_122023.rb + IFS=: + set /home/esp/esp2/mission . + fullPath=/home/esp/esp2/mission/DA_WCR_122023.rb + [ -f /home/esp/esp2/mission/DA_WCR_122023.rb ] + realpath /home/esp/esp2/mission/DA_WCR_122023.rb + return 0 + scriptPath=/home/esp/esp2local/mission/DA_WCR_122023.rb + exec ruby /home/esp/esp2/lib/esp.rb /home/esp/esp2local/mission/DA_WCR_122023.rb @13:19:24.57UTC20-Dec-23
ESP2 v3.42 I2C Gateway v3.20 for core bus @13:19:27.24 Configuration for short puck jake 1/11/21 by brent@mbari.org /home/esp/esp2/type/shallow/jake/configure.rb @13:19:30.92 Polling Can every 10:00 @13:19:31.17 Cleanly exited at 13:19:04UTC20-Dec-23 @13:19:31.22 Loading /home/esp/esp2local/mission/DA_WCR_122023.rb mission @13:19:31.28 GoodNews.email "Starting DA_WCR_122023 w/pucks in tube 7\n{1 => 33,\n 2 => 33,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 11,\n 7 => 20}\nCTD data unavailable\nCan@13:19:30, 31% flow, 16.3C, 35% humidity, 15.1psia, 13.734V, 0.284A, 263.249Ah, 3.91W",Subject: "DA_WCR_122023 begun" @13:19:31.32
Preparing to sleep until + 00:00:05 @13:19:31.35 Asleep til + 00:00:05 @13:19:31.37 Continuing at 13:19 @13:19:31.65 Gate.power :core,:OFF @13:19:31.66 Gate.power :raw,:OFF @13:19:31.67 Shutdown @13:19:34.87 GoodNews.email "Then, in tube 7: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_122023.rb:14\n{1 => 33,\n 2 => 33,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 11,\n 7 => 20}\nCTD data unavailable\nCan@13:19:31, 31% flow, 16.3C, 35% humidity, 15.1psia, 13.734V, 0.278A, 263.249Ah, 3.82W",Subject: "Asleep until 13:19" @13:19:36.22 upsync @13:19:36.29
Powering core bus @13:19:39.82 upsync @13:19:41.96
Gate.power :raw,:ON @13:19:42.88 CS.configure :default @13:19:42.93 PS.configure :default @13:19:42.98 SS.configure :default @13:19:43.03 CC.configure :default @13:19:43.08 PC.configure :default @13:19:43.15 Forearm.configure :loose @13:19:43.19 Elbow.configure :loose @13:19:43.26 SC.configure :loose @13:19:43.31 SE.configure :default @13:19:43.35 CH.configure :default @13:19:43.39 PH.configure :default @13:19:43.43 All dwarf controllers running firmware version 3.85 @13:19:43.54 Valve.reset @13:19:43.85 Intake.select :close @13:19:43.88 Exhaust.select :close @13:19:43.89 CTV.dial :air @13:19:43.96 PSV.dial :air @13:19:44.09
Forearm.home.seek :retract @13:19:44.80 Hand.close @13:19:44.83
Elbow.reconfigure :loose @13:19:44.92 Elbow.jog 1500 @13:19:45.19 PS.home.seek 0ml @13:19:45.26 CSV.dial :waste1 @13:19:45.35
Elbow.reconfigure :loose @13:19:45.39 Elbow.home.seek :clear @13:19:46.00 SSV.dial :spit,avoiding: :exhaust @13:19:46.88 CS.reconfigure :fast @13:19:46.92 SS.home.seek 0ml @13:19:47.01 CS.home.seek 0ml @13:19:48.67
SE.home.seek :down @13:19:49.30 SC.reconfigure :tight @13:19:49.35 SC.home.seek :initial @13:19:53.45 PS.stop @13:20:17.76
SC.configure :limp @13:20:17.81 Forearm.seek :retract @13:20:17.88 Elbow.seek :hammer @13:20:18.94 Forearm.reconfigure :tight @13:20:18.99 Forearm.seek retracted @13:20:19.64 PC.home.seek :open @13:20:19.65
CC.home.seek :open @13:20:23.06 Phase [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] @13:20:23.07 Notice.email "At 13:20:22 began in tube 7: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_122023.rb:14\n{1 => 33,\n 2 => 33,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 11,\n 7 => 20}\nCTD data unavailable\nCan@13:20:23, 31% flow, 16.4C, 35% humidity, 15.1psia, 13.730V, 0.290A, 263.254Ah, 3.99W",Subject: "Started DA_WCR_122023 phase initialPurge; da [1000, 1000] #da and wsr are run simultanelously" @13:20:23.10
Starting initialPurge @13:20:23.12 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @13:20:23.18 Hand.open @13:20:23.98 Elbow.reconfigure :tight @13:20:24.03 Elbow.seek FlushPuck::Garage @13:20:25.92 Elbow.reconfigure :loose @13:20:26.03 Forearm.reconfigure :loose @13:20:26.09 Forearm.seek :garage @13:20:27.13 Hand.close @13:20:27.53 upsync @13:20:27.86
Forearm.seek :retract @13:20:28.90 Elbow.reconfigure :tight @13:20:28.95 Elbow.seek CC @13:20:31.92 Elbow.reconfigure :loose @13:20:32.03 Forearm.reconfigure :tight @13:20:32.08 Forearm.seek CC @13:20:33.34 CC.seek :closed @13:20:37.97 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.45 @13:20:38.04 Hand.open @13:20:38.67 Forearm.reconfigure :loose @13:20:38.72 Forearm.seek :retract @13:20:39.71 Flushing the Collection Valve::Manifold @13:20:39.83 CSV.dial CSR @13:20:41.12 Pulling 5ml of CSR.flush @13:20:41.13 CSR.select :flush @13:20:41.25 CS.reconfigure :default @13:20:41.71 CS.seek 5ml @13:20:55.31 delay 5 seconds @13:21:00.33 CSR.select :air @13:21:00.50 CS.reconfigure :fast @13:21:00.55 CS.seek 7.5ml @13:21:07.31 CSV.dial :waste @13:21:08.60 CS.reconfigure :default @13:21:08.64 CS.seek 0ml @13:21:28.69 Purging external Intake @13:21:28.81 CSV.dial :flush @13:21:29.27 CS.reconfigure :fast @13:21:29.32 CS.seek 10ml @13:21:53.78 delay 5 seconds @13:21:58.80 CSV.dial CBV @13:21:58.84
CBV.dial CSV @13:21:59.64 CTV.dial :intake @13:22:00.43 Intake.select :open @13:22:00.55 CS.seek 0ml @13:22:24.15 Intake.select :close @13:22:24.26 CTV.dial :air @13:22:25.05 Flushing upper manifold @13:22:25.16 CSV.dial :flush @13:22:25.91 CS.seek 5ml @13:22:38.62 delay 5 seconds @13:22:43.64 CSV.dial :air @13:22:44.26 CS.seek 7ml @13:22:49.78 CSV.dial CBV @13:22:49.80
CBV.dial CSV @13:22:50.81 CTV.dial :air @13:22:50.89 CS.seek 0ml @13:23:07.53 delay 7 seconds @13:23:14.55 CS.seek 3ml @13:23:22.56 CSV.dial :waste @13:23:23.02 CS.seek 0ml @13:23:30.73 CSV.dial :flush @13:23:31.17 CS.seek 3ml @13:23:39.18 delay 5 seconds @13:23:44.20 CSV.dial :air @13:23:44.82 CS.seek 5ml @13:23:50.37 CSV.dial :air @13:23:50.45 CS.seek 0ml @13:24:02.62 delay 7 seconds @13:24:09.65 CS.seek 3ml @13:24:17.71 CSV.dial :waste @13:24:18.44 CS.seek 0ml @13:24:26.15 Garaging FlushPuck in Collection Clamp @13:24:26.22 Elbow.reconfigure :tight @13:24:26.27 Elbow.seek CC @13:24:26.57 Elbow.reconfigure :loose @13:24:26.67 Forearm.seek CC @13:24:27.71 Hand.close @13:24:28.36 CC.seek :open @13:24:33.43 Forearm.seek :retract @13:24:34.45 Elbow.reconfigure :tight @13:24:34.50 Elbow.seek :garage @13:24:37.32 Elbow.reconfigure :loose @13:24:37.42 Forearm.reconfigure :tight @13:24:37.47 Forearm.seek :garage @13:24:38.52 Hand.open @13:24:39.16 Forearm.reconfigure :loose @13:24:39.21 Forearm.seek :retract @13:24:40.19 da sampling at most 1000ml, wcr at most 1000ml @13:24:40.23 Loading sh1 puck from tube 7 into Collection Clamp @13:24:40.24 DAfans.email "Started da in tube 7 at 13:24:40UTC20-Dec-23\n{1 => 33,\n 2 => 33,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 11,\n 7 => 20}\nCTD data unavailable\nCan@13:20:23, 31% flow, 16.4C, 35% humidity, 15.1psia, 13.730V, 0.290A, 263.254Ah, 3.99W",Subject: "da sampling at most 1000ml, wcr at most 1000ml" @13:24:40.51
SC.reconfigure :tight @13:24:40.57 SC.seek 7 @13:24:44.56 upsync @13:24:50.43
SC.configure :limp @13:24:50.67 Elbow.reconfigure :tight @13:24:50.72 Elbow.seek 7 @13:24:52.66 Forearm.reconfigure :tight @13:24:52.71 Forearm.seek 7 + 450 counts @13:24:53.77 SE.reconfigure :pick @13:24:53.83 SE.seek :beamBreak @13:24:55.67 SE.configure :default @13:24:55.84 Elbow.seek 7 @13:24:56.16 Forearm.seek 7 + 300 counts @13:24:56.40 Forearm.reconfigure :loose @13:24:56.45 Hand.close @13:24:57.12 SE.seek :down @13:24:57.33
Forearm.seek :retract @13:24:58.58 SE.stop @13:24:58.72
Elbow.seek CC @13:25:00.29 Elbow.reconfigure :loose @13:25:00.39 Forearm.reconfigure :tight @13:25:00.44 Forearm.seek CC @13:25:01.76 CC.seek :closed @13:25:06.32 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.28 @13:25:06.33 Hand.open @13:25:07.04 Forearm.reconfigure :loose @13:25:07.09 Forearm.seek :retract @13:25:08.11 collectSample 1000ml w/bubble point of 24psi @13:25:08.12 Priming 7ml @13:25:08.13 Intake.select :close @13:25:08.14 Exhaust.select :close @13:25:08.15 SS.hold @13:25:08.37 Calibrating pressure and temperature sensors @13:25:08.40 CTV.dial :air @13:25:08.48 SSV.dial :spit,avoiding: :exhaust @13:25:08.56 SS.reconfigure :fast @13:25:08.61 SS.seek 5ml @13:25:11.60 SSV.dial CBV @13:25:11.62
CBV.dial SSV @13:25:12.97 delay 3.8 seconds @13:25:16.90 Sensor limits: Intake max 98.4psia; Outlet max 91.8psia @13:25:16.91 SSV.dial :spit @13:25:18.23 SS.seek 0ml @13:25:20.91 SSV.dial CBV @13:25:22.24 CTV.dial :intake @13:25:23.03 SSV.dial CBV @13:25:23.05
CBV.dial SSV @13:25:23.06 Allowing 40s for 7.0ml of remaining 7.0ml @13:25:23.08 Intake.select :open @13:25:25.25 Intake @14.8psia, vacuum from 9.3 to 12.3psi @13:25:25.26 SS.reconfigure :inhaling 2.20ml/s @13:25:25.31 SS.seek 7ml @13:25:32.17 Stroke completed -- waiting for pressure to equalize ... @13:25:32.18 SS.reconfigure :exhale @13:25:32.23 SS.hold @13:25:38.93 Intake.select :close @13:25:39.08 SSV.dial :exhaust @13:25:39.85 Exhaust.select :open @13:25:39.96 SS.seek 0ml @13:25:43.99 Exhaust.select :close @13:25:44.09 Sampling 1000.0ml @13:25:44.10 Intake.select :close @13:25:44.11 Exhaust.select :close @13:25:44.13 SS.reconfigure :default @13:25:44.17 SS.hold @13:25:44.38 CTV.dial :intake @13:25:44.45 SSV.dial CBV @13:25:44.47
CBV.dial SSV @13:25:45.24 Allowing 130s for 25.0ml of remaining 1000.0ml @13:25:45.25 Intake.select :open @13:25:47.42 Intake @15.5psia, vacuum from 10.0 to 13.0psi @13:25:47.43 SS.reconfigure :inhaling 2.20ml/s @13:25:47.48 SS.seek 25ml @13:26:02.56 Stroke completed -- waiting for pressure to equalize ... @13:26:02.57 SS.reconfigure :exhale @13:26:02.62 SS.hold @13:26:29.58 Intake.select :close @13:26:29.74 SSV.dial :exhaust @13:26:30.51 Exhaust.select :open @13:26:30.63 SS.seek 0ml @13:26:39.50 Exhaust.select :close @13:26:39.61 SSV.dial CBV @13:26:39.64
CBV.dial SSV @13:26:40.41 Allowing 130s for 25.0ml of remaining 975.0ml @13:26:40.42 Intake.select :open @13:26:42.59 Intake @15.9psia, vacuum from 10.0 to 13.0psi @13:26:42.61 SS.reconfigure :inhaling 2.20ml/s @13:26:42.65 SS.seek 25ml @13:26:54.93 Vacuum too high -- waiting up to 40 seconds @13:26:54.95 SS.reconfigure :exhale @13:26:55.00 SS.hold @13:27:00.26 Intake @17.1psia, vacuum from 10.0 to 13.0psi @13:27:00.28 SS.reconfigure :inhaling 1.76ml/s @13:27:00.33 SS.seek 25ml @13:27:04.30 Stroke completed -- waiting for pressure to equalize ... @13:27:04.31 SS.reconfigure :exhale @13:27:04.35 SS.hold @13:27:27.10 Intake.select :close @13:27:27.25 SSV.dial :exhaust @13:27:28.02 Exhaust.select :open @13:27:28.14 SS.seek 0ml @13:27:36.97 Exhaust.select :close @13:27:37.08 SSV.dial CBV @13:27:37.10
CBV.dial SSV @13:27:37.88 Allowing 130s for 25.0ml of remaining 950.0ml @13:27:37.89 Intake.select :open @13:27:40.05 Intake @15.8psia, vacuum from 10.0 to 13.0psi @13:27:40.07 SS.reconfigure :inhaling 1.76ml/s @13:27:40.11 SS.seek 25ml @13:27:57.64 Stroke completed -- waiting for pressure to equalize ... @13:27:57.65 SS.reconfigure :exhale @13:27:57.70 SS.hold @13:28:26.36 Intake.select :close @13:28:26.52 SSV.dial :exhaust @13:28:27.86 Exhaust.select :open @13:28:27.98 SS.seek 0ml @13:28:36.92 Exhaust.select :close @13:28:37.03 SSV.dial CBV @13:28:37.06
CBV.dial SSV @13:28:37.83 Allowing 130s for 25.0ml of remaining 925.0ml @13:28:37.85 Intake.select :open @13:28:40.01 Intake @16.0psia, vacuum from 10.0 to 13.0psi @13:28:40.03 SS.reconfigure :inhaling 1.76ml/s @13:28:40.07 SS.seek 25ml @13:28:56.43 Vacuum too high -- waiting up to 40 seconds @13:28:56.44 SS.reconfigure :exhale @13:28:56.49 SS.hold @13:29:01.06 Intake @16.7psia, vacuum from 10.0 to 13.0psi @13:29:01.08 SS.reconfigure :inhaling 1.41ml/s @13:29:01.13 SS.seek 25ml @13:29:01.68 Stroke completed -- waiting for pressure to equalize ... @13:29:01.69 SS.reconfigure :exhale @13:29:01.74 SS.hold @13:29:20.12 Intake.select :close @13:29:20.28 SSV.dial :exhaust @13:29:21.03 Exhaust.select :open @13:29:21.15 SS.seek 0ml @13:29:30.14 Exhaust.select :close @13:29:30.25 SSV.dial CBV @13:29:30.27
CBV.dial SSV @13:29:31.05 Allowing 130s for 25.0ml of remaining 900.0ml @13:29:31.06 Intake.select :open @13:29:33.66 Intake @16.2psia, vacuum from 10.0 to 13.0psi @13:29:33.68 SS.reconfigure :inhaling 1.41ml/s @13:29:33.73 SS.seek 25ml @13:29:53.81 Stroke completed -- waiting for pressure to equalize ... @13:29:53.82 SS.reconfigure :exhale @13:29:53.87 SS.hold @13:30:14.28 Intake.select :close @13:30:14.43 SSV.dial :exhaust @13:30:15.19 Exhaust.select :open @13:30:15.31 SS.seek 0ml @13:30:24.08 Exhaust.select :close @13:30:24.19 SSV.dial CBV @13:30:24.22
CBV.dial SSV @13:30:24.97 Allowing 130s for 25.0ml of remaining 875.0ml @13:30:24.98 Intake.select :open @13:30:27.15 Intake @16.2psia, vacuum from 10.0 to 13.0psi @13:30:27.16 SS.reconfigure :inhaling 1.41ml/s @13:30:27.21 SS.seek 25ml @13:30:47.82 Stroke completed -- waiting for pressure to equalize ... @13:30:47.83 SS.reconfigure :exhale @13:30:47.88 SS.hold @13:31:08.55 Intake.select :close @13:31:08.71 SSV.dial :exhaust @13:31:09.47 Exhaust.select :open @13:31:09.59 SS.seek 0ml @13:31:18.67 Exhaust.select :close @13:31:18.78 SSV.dial CBV @13:31:18.80
CBV.dial SSV @13:31:19.56 Allowing 130s for 25.0ml of remaining 850.0ml @13:31:19.57 Intake.select :open @13:31:21.74 Intake @16.1psia, vacuum from 10.0 to 13.0psi @13:31:21.76 SS.reconfigure :inhaling 1.41ml/s @13:31:21.80 SS.seek 25ml @13:31:42.06 Stroke completed -- waiting for pressure to equalize ... @13:31:42.07 SS.reconfigure :exhale @13:31:42.11 SS.hold @13:32:00.63 Intake.select :close @13:32:00.79 SSV.dial :exhaust @13:32:01.54 Exhaust.select :open @13:32:01.66 SS.seek 0ml @13:32:10.39 Exhaust.select :close @13:32:10.49 SSV.dial CBV @13:32:10.51
CBV.dial SSV @13:32:11.28 Allowing 130s for 25.0ml of remaining 825.0ml @13:32:11.29 Intake.select :open @13:32:13.46 Intake @16.1psia, vacuum from 10.0 to 13.0psi @13:32:13.48 SS.reconfigure :inhaling 1.41ml/s @13:32:13.52 SS.seek 25ml @13:32:34.12 Stroke completed -- waiting for pressure to equalize ... @13:32:34.13 SS.reconfigure :exhale @13:32:34.18 SS.hold @13:32:50.50 Intake.select :close @13:32:50.65 SSV.dial :exhaust @13:32:51.42 Exhaust.select :open @13:32:51.53 SS.seek 0ml @13:33:00.53 Exhaust.select :close @13:33:00.63 SSV.dial CBV @13:33:00.65
CBV.dial SSV @13:33:01.44 Allowing 130s for 25.0ml of remaining 800.0ml @13:33:01.45 Intake.select :open @13:33:03.61 Intake @16.1psia, vacuum from 10.0 to 13.0psi @13:33:03.63 SS.reconfigure :inhaling 1.41ml/s @13:33:03.67 SS.seek 25ml @13:33:23.65 Stroke completed -- waiting for pressure to equalize ... @13:33:23.66 SS.reconfigure :exhale @13:33:23.71 SS.hold @13:33:42.09 Intake.select :close @13:33:42.24 SSV.dial :exhaust @13:33:42.99 Exhaust.select :open @13:33:43.11 SS.seek 0ml @13:33:51.87 Exhaust.select :close @13:33:51.98 SSV.dial CBV @13:33:52.00
CBV.dial SSV @13:33:52.78 Allowing 130s for 25.0ml of remaining 775.0ml @13:33:52.79 Intake.select :open @13:33:54.95 Intake @16.2psia, vacuum from 10.0 to 13.0psi @13:33:54.97 SS.reconfigure :inhaling 1.41ml/s @13:33:55.01 SS.seek 25ml @13:34:15.43 Stroke completed -- waiting for pressure to equalize ... @13:34:15.44 SS.reconfigure :exhale @13:34:15.49 SS.hold @13:34:31.72 Intake.select :close @13:34:31.88 SSV.dial :exhaust @13:34:33.07 Exhaust.select :open @13:34:33.19 SS.seek 0ml @13:34:41.93 Exhaust.select :close @13:34:42.04 SSV.dial CBV @13:34:42.07
CBV.dial SSV @13:34:42.84 Allowing 130s for 25.0ml of remaining 750.0ml @13:34:42.85 Intake.select :open @13:34:45.02 Intake @16.1psia, vacuum from 10.0 to 13.0psi @13:34:45.04 SS.reconfigure :inhaling 1.41ml/s @13:34:45.09 SS.seek 25ml @13:35:05.58 Stroke completed -- waiting for pressure to equalize ... @13:35:05.60 SS.reconfigure :exhale @13:35:05.64 SS.hold @13:35:21.98 Intake.select :close @13:35:22.13 SSV.dial :exhaust @13:35:22.90 Exhaust.select :open @13:35:23.01 SS.seek 0ml @13:35:31.65 Exhaust.select :close @13:35:31.76 SSV.dial CBV @13:35:31.78
CBV.dial SSV @13:35:32.56 Allowing 130s for 25.0ml of remaining 725.0ml @13:35:32.57 Intake.select :open @13:35:34.73 Intake @16.3psia, vacuum from 10.0 to 13.0psi @13:35:34.74 SS.reconfigure :inhaling 1.41ml/s @13:35:34.79 SS.seek 25ml @13:35:55.26 Stroke completed -- waiting for pressure to equalize ... @13:35:55.27 SS.reconfigure :exhale @13:35:55.31 SS.hold @13:36:11.95 Intake.select :close @13:36:12.10 SSV.dial :exhaust @13:36:12.87 Exhaust.select :open @13:36:12.98 SS.seek 0ml @13:36:21.68 Exhaust.select :close @13:36:21.79 SSV.dial CBV @13:36:21.81
CBV.dial SSV @13:36:22.59 Allowing 130s for 25.0ml of remaining 700.0ml @13:36:22.60 Intake.select :open @13:36:24.77 Intake @16.2psia, vacuum from 10.0 to 13.0psi @13:36:24.78 SS.reconfigure :inhaling 1.41ml/s @13:36:24.83 SS.seek 25ml @13:36:45.46 Stroke completed -- waiting for pressure to equalize ... @13:36:45.47 SS.reconfigure :exhale @13:36:45.51 SS.hold @13:37:03.87 Intake.select :close @13:37:04.03 SSV.dial :exhaust @13:37:04.80 Exhaust.select :open @13:37:04.92 SS.seek 0ml @13:37:13.58 Exhaust.select :close @13:37:13.69 SSV.dial CBV @13:37:13.72
CBV.dial SSV @13:37:14.49 Allowing 130s for 25.0ml of remaining 675.0ml @13:37:14.50 Intake.select :open @13:37:16.67 Intake @16.2psia, vacuum from 10.0 to 13.0psi @13:37:16.69 SS.reconfigure :inhaling 1.41ml/s @13:37:16.74 SS.seek 25ml @13:37:34.42 Vacuum too high -- waiting up to 40 seconds @13:37:34.43 SS.reconfigure :exhale @13:37:34.49 SS.hold @13:37:41.24 Intake @15.8psia, vacuum from 10.0 to 13.0psi @13:37:41.25 SS.reconfigure :inhaling 1.13ml/s @13:37:41.31 SS.seek 25ml @13:37:44.97 Stroke completed -- waiting for pressure to equalize ... @13:37:44.98 SS.reconfigure :exhale @13:37:45.03 SS.hold @13:37:56.21 Intake.select :close @13:37:56.37 SSV.dial :exhaust @13:37:57.13 Exhaust.select :open @13:37:57.25 SS.seek 0ml @13:38:06.00 Exhaust.select :close @13:38:06.11 SSV.dial CBV @13:38:06.15
CBV.dial SSV @13:38:06.90 Allowing 130s for 25.0ml of remaining 650.0ml @13:38:06.91 Intake.select :open @13:38:09.08 Intake @16.2psia, vacuum from 10.0 to 13.0psi @13:38:09.09 SS.reconfigure :inhaling 1.13ml/s @13:38:09.14 SS.seek 25ml @13:38:33.91 Stroke completed -- waiting for pressure to equalize ... @13:38:33.92 SS.reconfigure :exhale @13:38:33.97 SS.hold @13:38:50.61 Intake.select :close @13:38:50.76 SSV.dial :exhaust @13:38:51.52 Exhaust.select :open @13:38:51.64 SS.seek 0ml @13:39:00.57 Exhaust.select :close @13:39:00.67 SSV.dial CBV @13:39:00.69
CBV.dial SSV @13:39:01.47 Allowing 130s for 25.0ml of remaining 625.0ml @13:39:01.49 Intake.select :open @13:39:03.65 Intake @16.2psia, vacuum from 10.0 to 13.0psi @13:39:03.66 SS.reconfigure :inhaling 1.13ml/s @13:39:03.71 SS.seek 25ml @13:39:27.95 Stroke completed -- waiting for pressure to equalize ... @13:39:27.96 SS.reconfigure :exhale @13:39:28.01 SS.hold @13:39:46.89 Intake.select :close @13:39:47.04 SSV.dial :exhaust @13:39:47.80 Exhaust.select :open @13:39:47.92 SS.seek 0ml @13:39:56.66 Exhaust.select :close @13:39:56.77 SSV.dial CBV @13:39:56.79
CBV.dial SSV @13:39:57.57 Allowing 130s for 25.0ml of remaining 600.0ml @13:39:57.58 Intake.select :open @13:39:59.74 Intake @16.2psia, vacuum from 10.0 to 13.0psi @13:39:59.75 SS.reconfigure :inhaling 1.13ml/s @13:39:59.80 SS.seek 25ml @13:40:16.26 Vacuum too high -- waiting up to 40 seconds @13:40:16.27 SS.reconfigure :exhale @13:40:16.33 SS.hold @13:40:23.89 Intake @16.8psia, vacuum from 10.0 to 13.0psi @13:40:23.91 SS.reconfigure :inhaling 0.91ml/s @13:40:23.96 SS.seek 25ml @13:40:34.58 Stroke completed -- waiting for pressure to equalize ... @13:40:34.59 SS.reconfigure :exhale @13:40:34.64 SS.hold @13:40:48.92 Intake.select :close @13:40:49.07 SSV.dial :exhaust @13:40:49.83 Exhaust.select :open @13:40:49.95 SS.seek 0ml @13:40:58.59 Exhaust.select :close @13:40:58.70 SSV.dial CBV @13:40:58.73
CBV.dial SSV @13:40:59.49 Allowing 130s for 25.0ml of remaining 575.0ml @13:40:59.50 Intake.select :open @13:41:01.67 Intake @16.2psia, vacuum from 10.0 to 13.0psi @13:41:01.68 SS.reconfigure :inhaling 0.91ml/s @13:41:01.73 SS.seek 25ml @13:41:28.85 Vacuum too high -- waiting up to 40 seconds @13:41:28.87 SS.reconfigure :exhale @13:41:28.92 SS.hold @13:41:36.93 Intake @16.6psia, vacuum from 10.0 to 13.0psi @13:41:36.95 SS.reconfigure :inhaling 0.73ml/s @13:41:37.00 SS.seek 25ml @13:41:40.50 Stroke completed -- waiting for pressure to equalize ... @13:41:40.51 SS.reconfigure :exhale @13:41:40.56 SS.hold @13:41:53.13 Intake.select :close @13:41:53.29 SSV.dial :exhaust @13:41:54.05 Exhaust.select :open @13:41:54.16 SS.seek 0ml @13:42:03.11 Exhaust.select :close @13:42:03.65 SSV.dial CBV @13:42:03.67
CBV.dial SSV @13:42:04.44 Allowing 130s for 25.0ml of remaining 550.0ml @13:42:04.45 Intake.select :open @13:42:06.62 Intake @16.2psia, vacuum from 10.0 to 13.0psi @13:42:06.64 SS.reconfigure :inhaling 0.73ml/s @13:42:06.68 SS.seek 25ml @13:42:42.69 Stroke completed -- waiting for pressure to equalize ... @13:42:42.70 SS.reconfigure :exhale @13:42:42.74 SS.hold @13:43:03.08 Intake.select :close @13:43:03.24 SSV.dial :exhaust @13:43:04.01 Exhaust.select :open @13:43:04.12 SS.seek 0ml @13:43:12.83 Exhaust.select :close @13:43:12.93 SSV.dial CBV @13:43:12.95
CBV.dial SSV @13:43:13.73 Allowing 130s for 25.0ml of remaining 525.0ml @13:43:13.75 Intake.select :open @13:43:15.91 Intake @16.3psia, vacuum from 10.0 to 13.0psi @13:43:15.92 SS.reconfigure :inhaling 0.73ml/s @13:43:16.40 SS.seek 25ml @13:43:47.26 Vacuum too high -- waiting up to 40 seconds @13:43:47.27 SS.reconfigure :exhale @13:43:47.32 SS.hold @13:43:54.95 Intake @16.8psia, vacuum from 10.0 to 13.0psi @13:43:54.96 SS.reconfigure :inhaling 0.59ml/s @13:43:55.01 SS.seek 25ml @13:44:02.36 Stroke completed -- waiting for pressure to equalize ... @13:44:02.37 SS.reconfigure :exhale @13:44:02.41 SS.hold @13:44:14.99 Intake.select :close @13:44:15.14 SSV.dial :exhaust @13:44:15.90 Exhaust.select :open @13:44:16.02 SS.seek 0ml @13:44:24.98 Exhaust.select :close @13:44:25.10 SSV.dial CBV @13:44:25.12
CBV.dial SSV @13:44:25.87 Allowing 130s for 25.0ml of remaining 500.0ml @13:44:25.89 Intake.select :open @13:44:28.06 Intake @16.2psia, vacuum from 10.0 to 13.0psi @13:44:28.07 SS.reconfigure :inhaling 0.59ml/s @13:44:28.12 SS.seek 25ml @13:45:12.68 Stroke completed -- waiting for pressure to equalize ... @13:45:12.69 SS.reconfigure :exhale @13:45:12.74 SS.hold @13:45:33.17 Intake.select :close @13:45:33.33 SSV.dial :exhaust @13:45:34.10 Exhaust.select :open @13:45:34.21 SS.seek 0ml @13:45:42.95 Exhaust.select :close @13:45:43.06 SSV.dial CBV @13:45:43.08
CBV.dial SSV @13:45:43.86 Allowing 130s for 25.0ml of remaining 475.0ml @13:45:43.87 Intake.select :open @13:45:46.04 Intake @16.2psia, vacuum from 10.0 to 13.0psi @13:45:46.05 SS.reconfigure :inhaling 0.59ml/s @13:45:46.10 SS.seek 25ml @13:46:28.22 Vacuum too high -- waiting up to 40 seconds @13:46:28.23 SS.reconfigure :exhale @13:46:28.29 SS.hold @13:46:39.57 Intake @17.1psia, vacuum from 10.0 to 13.0psi @13:46:39.59 SS.reconfigure :inhaling 0.47ml/s @13:46:39.64 SS.seek 25ml @13:46:43.21 Stroke completed -- waiting for pressure to equalize ... @13:46:43.22 SS.reconfigure :exhale @13:46:43.27 SS.hold @13:46:56.16 Intake.select :close @13:46:56.31 SSV.dial :exhaust @13:46:57.07 Exhaust.select :open @13:46:57.19 SS.seek 0ml @13:47:05.95 Exhaust.select :close @13:47:06.05 SSV.dial CBV @13:47:06.07
CBV.dial SSV @13:47:06.84 Allowing 130s for 25.0ml of remaining 450.0ml @13:47:06.85 Intake.select :open @13:47:09.02 Intake @16.2psia, vacuum from 10.0 to 13.0psi @13:47:09.03 SS.reconfigure :inhaling 0.47ml/s @13:47:09.08 SS.seek 25ml @13:48:04.59 Stroke completed -- waiting for pressure to equalize ... @13:48:04.60 SS.reconfigure :exhale @13:48:04.64 SS.hold @13:48:27.09 Intake.select :close @13:48:27.24 SSV.dial :exhaust @13:48:28.01 Exhaust.select :open @13:48:28.12 SS.seek 0ml @13:48:37.10 Exhaust.select :close @13:48:37.21 SSV.dial CBV @13:48:37.23
CBV.dial SSV @13:48:38.01 Allowing 130s for 25.0ml of remaining 425.0ml @13:48:38.02 Intake.select :open @13:48:40.19 Intake @16.2psia, vacuum from 10.0 to 13.0psi @13:48:40.21 SS.reconfigure :inhaling 0.47ml/s @13:48:40.25 SS.seek 25ml @13:49:35.58 Stroke completed -- waiting for pressure to equalize ... @13:49:35.59 SS.reconfigure :exhale @13:49:35.64 SS.hold @13:50:02.18 Intake.select :close @13:50:02.33 SSV.dial :exhaust @13:50:03.09 Exhaust.select :open @13:50:03.20 SS.seek 0ml @13:50:12.01 Exhaust.select :close @13:50:12.11 SSV.dial CBV @13:50:12.13
CBV.dial SSV @13:50:12.90 Allowing 130s for 25.0ml of remaining 400.0ml @13:50:12.91 Intake.select :open @13:50:15.08 Intake @16.2psia, vacuum from 10.0 to 13.0psi @13:50:15.10 SS.reconfigure :inhaling 0.47ml/s @13:50:15.14 SS.seek 25ml @13:51:10.53 Vacuum too high -- waiting up to 40 seconds @13:51:10.54 SS.reconfigure :exhale @13:51:10.60 SS.hold @13:51:27.47 Intake @17.6psia, vacuum from 10.0 to 13.0psi @13:51:27.49 SS.reconfigure :inhaling 0.38ml/s @13:51:27.54 SS.seek 25ml @13:51:27.74 Stroke completed -- waiting for pressure to equalize ... @13:51:27.75 SS.reconfigure :exhale @13:51:27.80 SS.hold @13:51:40.37 Intake.select :close @13:51:40.52 SSV.dial :exhaust @13:51:41.29 Exhaust.select :open @13:51:41.40 SS.seek 0ml @13:51:50.19 Exhaust.select :close @13:51:50.68 SSV.dial CBV @13:51:50.70
CBV.dial SSV @13:51:51.47 Allowing 130s for 25.0ml of remaining 375.0ml @13:51:51.49 Intake.select :open @13:51:53.65 Intake @16.3psia, vacuum from 10.0 to 13.0psi @13:51:53.67 SS.reconfigure :inhaling 0.38ml/s @13:51:53.72 SS.seek 25ml @13:53:02.31 Stroke completed -- waiting for pressure to equalize ... @13:53:02.32 SS.reconfigure :exhale @13:53:02.37 SS.hold @13:53:30.99 Intake.select :close @13:53:31.14 SSV.dial :exhaust @13:53:31.91 Exhaust.select :open @13:53:32.45 SS.seek 0ml @13:53:41.25 Exhaust.select :close @13:53:41.36 SSV.dial CBV @13:53:41.39
CBV.dial SSV @13:53:42.16 Allowing 130s for 25.0ml of remaining 350.0ml @13:53:42.17 Intake.select :open @13:53:44.34 Intake @16.2psia, vacuum from 10.0 to 13.0psi @13:53:44.36 SS.reconfigure :inhaling 0.38ml/s @13:53:44.41 SS.seek 25ml @13:54:52.91 Stroke completed -- waiting for pressure to equalize ... @13:54:52.92 SS.reconfigure :exhale @13:54:52.96 SS.hold @13:55:28.14 Intake.select :close @13:55:28.30 SSV.dial :exhaust @13:55:29.06 Exhaust.select :open @13:55:29.18 SS.seek 0ml @13:55:38.22 Exhaust.select :close @13:55:38.33 SSV.dial CBV @13:55:38.35
CBV.dial SSV @13:55:39.13 Allowing 130s for 25.0ml of remaining 325.0ml @13:55:39.14 Intake.select :open @13:55:41.31 Intake @16.2psia, vacuum from 10.0 to 13.0psi @13:55:41.33 SS.reconfigure :inhaling 0.38ml/s @13:55:41.38 SS.seek 25ml @13:56:42.96 Vacuum too high -- waiting up to 40 seconds @13:56:42.97 SS.reconfigure :exhale @13:56:43.03 SS.hold @13:57:03.54 Intake @17.1psia, vacuum from 10.0 to 13.0psi @13:57:03.56 SS.reconfigure :inhaling 0.31ml/s @13:57:04.03 SS.seek 25ml @13:57:12.53 Stroke completed -- waiting for pressure to equalize ... @13:57:12.54 SS.reconfigure :exhale @13:57:12.59 SS.hold @13:57:35.03 Intake.select :close @13:57:35.18 SSV.dial :exhaust @13:57:35.94 Exhaust.select :open @13:57:36.05 SS.seek 0ml @13:57:45.11 Exhaust.select :close @13:57:45.21 SSV.dial CBV @13:57:45.26
CBV.dial SSV @13:57:45.98 Allowing 130s for 25.0ml of remaining 300.0ml @13:57:46.00 Intake.select :open @13:57:48.16 Intake @16.3psia, vacuum from 10.0 to 13.0psi @13:57:48.18 SS.reconfigure :inhaling 0.31ml/s @13:57:48.23 SS.seek 25ml @13:59:14.81 Stroke completed -- waiting for pressure to equalize ... @13:59:14.82 SS.reconfigure :exhale @13:59:14.87 SS.hold @13:59:55.53 Intake.select :close @13:59:56.03 SSV.dial :exhaust @13:59:56.87 Exhaust.select :open @13:59:56.99 SS.seek 0ml @14:00:06.03 Exhaust.select :close @14:00:06.13 SSV.dial CBV @14:00:06.15
CBV.dial SSV @14:00:06.94 Allowing 130s for 25.0ml of remaining 275.0ml @14:00:06.95 Intake.select :open @14:00:09.12 Intake @16.1psia, vacuum from 10.0 to 13.0psi @14:00:09.13 SS.reconfigure :inhaling 0.31ml/s @14:00:09.18 SS.seek 25ml @14:01:37.26 Stroke completed -- waiting for pressure to equalize ... @14:01:37.27 SS.reconfigure :exhale @14:01:37.32 SS.hold @14:02:16.96 SS.reconfigure :default @14:02:17.00 SS.hold @14:02:17.08 Intake.select :close @14:02:17.24 ShallowSampler::Clogged in MAIN -- Filtering too Slowly after 750.0ml @14:02:17.25 SSV.dial :exhaust @14:02:18.01 Exhaust.select :open @14:02:18.13 SS.reconfigure :exhale @14:02:18.17 SS.seek 0ml @14:02:26.95 Exhaust.select :close @14:02:27.05 Sampled 750.0ml @14:02:27.07 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @14:02:27.08 Intake.select :close @14:02:27.14 CSV.dial :waste @14:02:27.27 SSV.dial CBV,avoiding: :exhaust @14:02:27.29
CBV.dial SSV @14:02:27.37 CS.seek 0ml @14:02:27.44 CSV.dial :air @14:02:28.18 CS.seek 10ml @14:02:29.20
CTV.dial :air @14:02:29.99 delay 15 seconds @14:02:45.01 SSV.dial :spit,avoiding: :exhaust @14:02:46.34 CTV.dial CSV,avoiding: :intake @14:02:46.39
SS.seek 0ml @14:02:46.50 SSV.dial CBV,avoiding: :exhaust @14:02:52.74 SSV.dial between CBV and plug @14:02:53.22 CBV.dial between SSV and waste2 @14:02:53.60 CSV.dial between air and CTV @14:02:53.92 CS.seek 8.391ml @14:02:58.53 CTV.dial CSV,avoiding: :air @14:02:58.57
CSV.dial CTV @14:02:58.92 CS.reconfigure :pressurize @14:02:58.97 CS.seek 0ml @14:03:13.50 BubblePoint: Intake @24.1psia, Outlet @1.0psia @14:03:13.51 CBV.dial SSV @14:03:13.85 SS.reconfigure :default @14:03:13.90 SS.seek 10ml @14:03:19.51 delay 15 seconds @14:03:34.64 Evacuated: Intake @23.7psia, Outlet @23.4psia @14:03:34.65 SSV.dial :spit,avoiding: :exhaust @14:03:36.11 SS.reconfigure :exhale @14:03:36.16 SS.seek 0ml @14:03:41.07 SS.reconfigure :fast @14:03:41.11 SS.seek 5ml @14:03:43.86 SSV.dial CBV,avoiding: :exhaust @14:03:43.90
CBV.dial SSV @14:03:45.20 CTV.dial :air,avoiding: :intake @14:03:46.55 CSV.dial CTV @14:03:46.61
SS.seek 10ml @14:03:47.72 CSV.dial :air @14:03:48.18 CS.reconfigure :fast @14:03:48.23 CS.seek 0ml @14:03:49.18
SSV.dial :spit,avoiding: :exhaust @14:03:50.51 SS.reconfigure :exhale @14:03:50.56 SS.seek 0ml @14:03:55.58 SS.reconfigure :fast @14:03:55.63 SS.seek 5ml @14:03:55.77 CS.stop @14:03:58.44 SSV.dial CBV,avoiding: :exhaust @14:03:58.46
CBV.dial SSV @14:04:00.81 SSV.dial :spit,avoiding: :exhaust @14:04:02.15 SS.reconfigure :default @14:04:02.20 SS.seek 0ml @14:04:05.43 SS.stop @14:04:05.47 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @14:04:05.48 Intake.select :close @14:04:05.55 CSV.dial :waste @14:04:05.68 SSV.dial CBV,avoiding: :exhaust @14:04:05.70
CBV.dial SSV @14:04:06.34 CS.seek 0ml @14:04:06.40 CSV.dial :air @14:04:07.04
CTV.dial :air @14:04:07.12 delay 15 seconds @14:04:07.16 CS.seek 10ml @14:04:22.14
SSV.dial :spit,avoiding: :exhaust @14:04:23.47 CTV.dial CSV,avoiding: :intake @14:04:23.52
SS.reconfigure :exhale @14:04:23.59 SS.seek 0ml @14:04:23.96 SSV.dial CBV,avoiding: :exhaust @14:04:31.71 SSV.dial between CBV and plug @14:04:32.20 CBV.dial between SSV and waste2 @14:04:32.60 CSV.dial between air and CTV @14:04:32.90 CS.seek 7.587ml @14:04:39.25 CTV.dial CSV,avoiding: :air @14:04:39.27
CSV.dial CTV @14:04:39.98 CS.reconfigure :pressurize @14:04:40.03 CS.seek 0ml @14:04:56.13 BubblePoint: Intake @24.2psia, Outlet @3.3psia @14:04:56.14 CBV.dial SSV @14:04:56.46 SS.reconfigure :default @14:04:56.51 SS.seek 10ml @14:05:02.13 delay 15 seconds @14:05:17.26 Evacuated: Intake @23.9psia, Outlet @23.7psia @14:05:17.27 SSV.dial :spit,avoiding: :exhaust @14:05:18.31 SS.reconfigure :exhale @14:05:18.36 SS.seek 0ml @14:05:23.37 SS.reconfigure :fast @14:05:23.41 SS.seek 5ml @14:05:26.34 SSV.dial CBV,avoiding: :exhaust @14:05:26.36
CBV.dial SSV @14:05:27.70 CTV.dial :air,avoiding: :intake @14:05:28.99 CSV.dial CTV @14:05:29.04
SS.seek 10ml @14:05:30.20 CSV.dial :air @14:05:30.65 CS.reconfigure :fast @14:05:30.70 CS.seek 0ml @14:05:31.66
SSV.dial :spit,avoiding: :exhaust @14:05:32.98 SS.reconfigure :exhale @14:05:33.03 SS.seek 0ml @14:05:34.95 CS.stop @14:05:38.03
SS.reconfigure :fast @14:05:38.08 SS.seek 5ml @14:05:40.86 SSV.dial CBV,avoiding: :exhaust @14:05:40.88
CBV.dial SSV @14:05:43.22 SSV.dial :spit,avoiding: :exhaust @14:05:44.54 SS.reconfigure :default @14:05:44.59 SS.seek 0ml @14:05:47.94 SS.stop @14:05:47.98 Lysing: Adding 2.0ml AqMeOH @14:05:47.99 CBV.dial between CSV and waste1,avoiding: :DBV @14:05:49.49 CTV.dial CSV @14:05:50.82 CSV.dial CSR @14:05:51.61 Pulling 1ml of CSR.AqMeOH @14:05:51.62 CSR.select :AqMeOH @14:05:51.74 CS.reconfigure :default @14:05:51.78 CS.seek 1ml @14:05:55.21 delay 5 seconds @14:06:00.22 CSR.select :air @14:06:00.39 CS.seek 6ml @14:06:13.99 delay 2 seconds @14:06:16.00 CSV.dial :waste2 @14:06:16.57 CS.reconfigure :fast @14:06:16.62 CS.seek 0ml @14:06:31.08 CSV.dial CSR @14:06:31.94 Pulling 2ml of CSR.AqMeOH @14:06:31.95 CSR.select :AqMeOH @14:06:32.06 CS.reconfigure :default @14:06:32.11 CS.seek 2ml @14:06:38.02 delay 5 seconds @14:06:43.04 CSR.select :air @14:06:43.21 CS.seek 5ml @14:06:51.67 delay 2 seconds @14:06:53.69 CSV.dial CTV @14:06:54.15 CS.seek 1ml @14:07:05.31 Before lysing Collection Puck Heater at 13.3C/29.3psia @14:07:05.33 Heating Puck.from 7, :sh1 in Collection Clamp to 70C for 10:00 @14:07:05.35 CH.seek 70C,8:00 @14:09:22.63 CH.hold 10:00,70C @14:09:22.83 Begun lysing Collection Puck Heater at 68C/36.1psia @14:19:22.84 Ended lysing Collection Puck Heater at 70C/35.2psia @14:19:22.86 CS.seek 0ml @14:19:26.45 CTV.dial between spare and air,avoiding: :intake @14:19:27.48 CSV.dial :air @14:19:27.92 CS.seek 5ml @14:19:41.72 CSV.dial CTV @14:19:42.18 delay 2 seconds @14:19:44.20 CSV.dial :air @14:19:44.65 delay 2 seconds @14:19:46.66 CSV.dial CTV @14:19:47.12 delay 2 seconds @14:19:49.13 CSV.dial :air @14:19:49.59 delay 2 seconds @14:19:51.61 CS.seek 0ml @14:20:05.46 Puck @74.9psia after final lysis pressurization @14:20:05.48 Recovering Lysate into Collection syringe @14:20:05.49 CSV.dial CBV @14:20:06.49 CBV.dial CSV @14:20:06.88 CS.seek 5ml @14:20:20.66 delay 5 seconds @14:20:25.68 CTV.dial :air @14:20:26.23 CS.seek 7ml @14:20:32.23 delay 5 seconds @14:20:37.25 CSV.dial :air2 @14:20:37.70 CS.seek 10ml @14:20:46.23 CSV.dial between air and CTV,avoiding: CBV @14:20:47.38 Lysing Complete @14:20:47.45 Storing sh1 puck in Collection Clamp to tube 6 @14:20:47.63 SC.reconfigure :tight @14:20:47.68 SC.seek 6 @14:20:51.24 SC.configure :limp @14:20:51.46 Elbow.reconfigure :tight @14:20:51.51 Elbow.seek 6 @14:20:53.27 Forearm.reconfigure :tight @14:20:53.32 Forearm.seek 6 + 450 counts @14:20:54.34 SE.reconfigure :pick @14:20:54.40 SE.seek :beamBreak @14:20:56.85 SE.configure :default @14:20:57.02 Elbow.reconfigure :limp @14:20:57.07 Forearm.reconfigure :loose @14:20:57.11 Forearm.seek 6 @14:20:57.38 Elbow.hold @14:20:57.44 Elbow.reconfigure :tight @14:20:57.54 SE.seek 10.11 Pucks below Top Plate @14:20:58.10 Forearm.force -20 @14:20:58.14 Forearm.seek 6 + 298 counts @14:20:58.43 Forearm.reconfigure :tight @14:20:58.48 Forearm.seek 6 + 450 counts @14:20:58.66 SE.seek 11.11 Pucks below Top Plate @14:20:59.26 Forearm.reconfigure :loose @14:20:59.31 Forearm.seek :retract @14:21:00.24 Elbow.seek CC @14:21:01.92 Elbow.reconfigure :loose @14:21:02.03 Forearm.seek CC @14:21:03.41 Hand.close @14:21:04.07 CC.seek :open @14:21:08.84 Forearm.seek :retract @14:21:09.83 Elbow.reconfigure :tight @14:21:09.88 Elbow.seek 6 @14:21:11.63 Elbow.reconfigure :limp @14:21:11.68 Forearm.seek 6 @14:21:12.73 Elbow.hold @14:21:12.80 Elbow.reconfigure :tight @14:21:12.85 Hand.open @14:21:13.56 Forearm.force -20 @14:21:13.60 Forearm.seek 6 + 311 counts @14:21:13.90 Forearm.reconfigure :tight @14:21:13.95 Forearm.seek 6 + 450 counts @14:21:14.21 SE.seek :down @14:21:15.34
Forearm.reconfigure :loose @14:21:15.39 Forearm.seek :retract @14:21:16.18 SE.stop @14:21:16.32
Tranferring DA @14:21:16.33 Beginning samplePassC @14:21:16.34 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:21:16.53 Elbow.seek FlushPuck::Garage @14:21:18.28 Elbow.reconfigure :loose @14:21:18.38 Forearm.seek :garage @14:21:19.42 Hand.close @14:21:20.18 Forearm.seek :retract @14:21:21.19 Elbow.reconfigure :tight @14:21:21.24 Elbow.seek CC @14:21:24.61 Elbow.reconfigure :loose @14:21:24.71 Forearm.reconfigure :tight @14:21:24.76 Forearm.seek CC @14:21:26.03 CC.seek :closed @14:21:30.57 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.40 @14:21:30.64 Hand.open @14:21:31.25 Forearm.reconfigure :loose @14:21:31.30 Forearm.seek :retract @14:21:32.33 PSV.dial PSR @14:21:33.86 Pulling 1.5ml of PSR.flush @14:21:33.87 PSR.select :flush @14:21:34.00 PS.seek 1.5ml @14:21:50.23 delay 5 seconds @14:21:55.25 PSV.dial :waste1 @14:21:55.73 PS.seek 0ml @14:22:11.82 PSV.dial PSR @14:22:12.29 PSR.select :air @14:22:12.40 PS.seek 2.5ml @14:22:38.80 PRV.dial CBV,avoiding: :ab2 @14:22:38.81
CBV.dial PRV @14:22:40.60 PSV.dial :mixloop @14:22:41.65 CTV.dial :air,avoiding: :intake @14:22:41.72 PTV.dial :PRVmixing @14:22:43.06 PS.seek 0ml @14:23:09.44 PSV.dial :air @14:23:09.92 PS.seek 2.5ml @14:23:36.22 PSV.dial :mixloop @14:23:36.72 PS.seek 0ml @14:24:03.00 PSV.dial :air @14:24:03.93 PS.seek 2.5ml @14:24:30.25 PSV.dial :mixloop @14:24:30.73 PS.seek 0ml @14:24:57.13 Garaging FlushPuck in Collection Clamp @14:24:57.21 Elbow.reconfigure :tight @14:24:57.25 Elbow.seek CC @14:24:57.54 Elbow.reconfigure :loose @14:24:57.64 Forearm.seek CC @14:24:58.67 Hand.close @14:24:59.32 CC.seek :open @14:25:03.96 Forearm.seek :retract @14:25:04.95 Elbow.reconfigure :tight @14:25:05.00 Elbow.seek :garage @14:25:07.85 Elbow.reconfigure :loose @14:25:07.95 Forearm.reconfigure :tight @14:25:08.00 Forearm.seek :garage @14:25:09.06 Hand.open @14:25:09.74 Forearm.reconfigure :loose @14:25:09.79 Forearm.seek :retract @14:25:10.79 PSV.dial :mixloop @14:25:10.86 PTV.dial :PRVmixing @14:25:10.95 PRV.dial CBV @14:25:10.98
CBV.dial PRV @14:25:11.17 CTV.dial :air,avoiding: :intake @14:25:11.26 Loading lyfil puck from tube 7 into Collection Clamp @14:25:11.49 SC.reconfigure :tight @14:25:11.54 SC.seek 7 @14:25:15.10 SC.configure :limp @14:25:15.32 Elbow.reconfigure :tight @14:25:15.37 Elbow.seek 7 @14:25:17.33 Forearm.reconfigure :tight @14:25:17.38 Forearm.seek 7 + 450 counts @14:25:18.94 SE.reconfigure :pick @14:25:18.99 SE.seek :beamBreak @14:25:20.98 SE.configure :default @14:25:21.15 Elbow.seek 7 @14:25:21.34 Forearm.seek 7 + 300 counts @14:25:21.48 Forearm.reconfigure :loose @14:25:21.53 Hand.close @14:25:22.20 SE.seek :down @14:25:22.40
Forearm.seek :retract @14:25:23.34 Elbow.seek CC @14:25:23.77 SE.stop @14:25:24.96
Elbow.reconfigure :loose @14:25:25.07 Forearm.reconfigure :tight @14:25:25.12 Forearm.seek CC @14:25:26.34 CC.seek :closed @14:25:30.88 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.17 @14:25:30.89 Hand.open @14:25:31.50 Forearm.reconfigure :loose @14:25:31.54 Forearm.seek :retract @14:25:32.50 CTV.dial CSV,avoiding: :intake @14:25:33.84 CSV.dial CTV @14:25:34.18 PS.seek 2.5ml @14:26:00.78 CS.seek 4.5ml @14:26:16.19 delay 8 seconds @14:26:24.21 PSV.dial :air @14:26:24.68 delay 2 seconds @14:26:26.69 PSV.dial :waste2 @14:26:27.45 PS.seek 0ml @14:26:53.75 CBV.dial between PRV and waste1 @14:26:54.08 CSV.dial :air @14:26:54.55 CS.seek 10ml @14:27:09.43 CSV.dial CTV @14:27:09.90 CS.seek 0ml @14:27:36.28 CBV.dial :waste1 @14:27:36.59 delay 5 seconds @14:27:41.62 CTV.dial :air,avoiding: :intake @14:27:43.03 Storing lyfil puck in Collection Clamp to tube 6 @14:27:43.21 SC.reconfigure :tight @14:27:43.26 SC.seek 6 @14:27:46.81 SC.configure :limp @14:27:47.03 Elbow.reconfigure :tight @14:27:47.08 Elbow.seek 6 @14:27:48.83 Forearm.reconfigure :tight @14:27:48.88 Forearm.seek 6 + 450 counts @14:27:49.93 SE.reconfigure :pick @14:27:49.99 SE.seek :beamBreak @14:27:52.29 SE.configure :default @14:27:52.46 Elbow.reconfigure :limp @14:27:52.51 Forearm.reconfigure :loose @14:27:52.55 Forearm.seek 6 @14:27:52.79 Elbow.hold @14:27:52.86 Elbow.reconfigure :tight @14:27:52.96 SE.seek 11.1 Pucks below Top Plate @14:27:53.53 Forearm.force -20 @14:27:53.57 Forearm.seek 6 + 313 counts @14:27:54.31 Forearm.reconfigure :tight @14:27:54.36 Forearm.seek 6 + 450 counts @14:27:54.55 SE.seek 12.1 Pucks below Top Plate @14:27:55.16 Forearm.reconfigure :loose @14:27:55.21 Forearm.seek :retract @14:27:56.14 Elbow.seek CC @14:27:57.83 Elbow.reconfigure :loose @14:27:57.93 Forearm.seek CC @14:27:58.90 Hand.close @14:27:59.56 CC.seek :open @14:28:04.30 Forearm.seek :retract @14:28:05.30 Elbow.reconfigure :tight @14:28:05.34 Elbow.seek 6 @14:28:07.10 Elbow.reconfigure :limp @14:28:07.15 Forearm.seek 6 @14:28:08.14 Elbow.hold @14:28:08.20 Elbow.reconfigure :tight @14:28:08.25 Hand.open @14:28:08.93 Forearm.force -20 @14:28:08.97 Forearm.seek 6 + 305 counts @14:28:09.27 Forearm.reconfigure :tight @14:28:09.32 Forearm.seek 6 + 450 counts @14:28:09.56 SE.seek :down @14:28:10.69
Forearm.reconfigure :loose @14:28:10.74 Forearm.seek :retract @14:28:11.44 SE.stop @14:28:11.95
PSV.dial :air @14:28:12.71 PS.seek 2.5ml @14:28:39.00 delay 1 second @14:28:40.02 PSV.dial :mixloop @14:28:40.47 delay 1 second @14:28:41.49 PSV.dial :air @14:28:41.96 delay 1 second @14:28:42.97 PSV.dial :mixloop @14:28:43.44 delay 1 second @14:28:44.46 PSV.dial :air @14:28:44.92 delay 1 second @14:28:45.95 PS.seek 0ml @14:29:12.25 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @14:29:12.44 Elbow.seek FlushPuck::Garage @14:29:14.27 Elbow.reconfigure :loose @14:29:14.37 Forearm.seek :garage @14:29:15.40 Hand.close @14:29:16.18 Forearm.seek :retract @14:29:17.19 Elbow.reconfigure :tight @14:29:17.23 Elbow.seek PC @14:29:18.52 Elbow.reconfigure :loose @14:29:18.63 Forearm.reconfigure :tight @14:29:18.67 Forearm.seek PC @14:29:19.89 PC.seek :closed @14:29:24.51 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.66 @14:29:24.57 Hand.open @14:29:25.24 Forearm.reconfigure :loose @14:29:25.29 Forearm.seek :retract @14:29:26.68 PRV.dial :air @14:29:27.16 PSV.dial :mixloop @14:29:27.69 PS.seek 0.3ml @14:29:31.59 PSV.dial PSR @14:29:32.66 Pulling 0.4ml of PSR.air @14:29:32.67 PSR.select :air @14:29:32.70 PS.seek 0.7ml @14:29:37.56 delay 3 seconds @14:29:40.58 PSR.select :air @14:29:40.60 PS.seek 2.5ml @14:29:59.84 delay 3 seconds @14:30:02.86 PSV.dial :mixloop @14:30:03.92 PTV.dial :mixing @14:30:04.69 PBV.dial :waste2 @14:30:05.66 PS.seek 0ml @14:30:32.00 Garaging FlushPuck in Processing Clamp @14:30:32.08 Elbow.reconfigure :tight @14:30:32.12 Elbow.seek PC @14:30:32.25 Elbow.reconfigure :loose @14:30:32.35 Forearm.seek PC @14:30:33.32 Hand.close @14:30:33.94 PC.seek :open @14:30:38.65 Forearm.seek :retract @14:30:39.62 Elbow.reconfigure :tight @14:30:39.67 Elbow.seek :garage @14:30:41.11 Elbow.reconfigure :loose @14:30:41.21 Forearm.reconfigure :tight @14:30:41.26 Forearm.seek :garage @14:30:42.32 Hand.open @14:30:42.99 Forearm.reconfigure :loose @14:30:43.04 Forearm.seek :retract @14:30:44.03 Loading da puck from tube 7 into Processing Clamp @14:30:44.27 SC.reconfigure :tight @14:30:44.32 SC.seek 7 @14:30:47.83 SC.configure :limp @14:30:48.05 Elbow.reconfigure :tight @14:30:48.52 Elbow.seek 7 @14:30:50.46 Forearm.reconfigure :tight @14:30:50.51 Forearm.seek 7 + 450 counts @14:30:51.54 SE.reconfigure :pick @14:30:51.60 SE.seek :beamBreak @14:30:53.62 SE.configure :default @14:30:53.78 Elbow.seek 7 @14:30:53.96 Forearm.seek 7 + 300 counts @14:30:54.16 Forearm.reconfigure :loose @14:30:54.21 Hand.close @14:30:54.82 SE.seek :down @14:30:55.02
Forearm.seek :retract @14:30:55.96 Elbow.seek PC @14:30:56.44 SE.stop @14:30:58.49
Elbow.reconfigure :loose @14:30:58.59 Forearm.reconfigure :tight @14:30:58.64 Forearm.seek PC @14:30:59.88 PC.seek :closed @14:31:04.59 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.64 @14:31:04.60 Hand.open @14:31:05.24 Forearm.reconfigure :loose @14:31:05.29 Forearm.seek :retract @14:31:06.26 Heating Puck.from 7, :da in Processing Clamp to 28C for 2:00:00 @14:31:06.28 PH.seek 28C,5:00 @14:31:49.02 PH.hold 2:00:00,28C @14:31:49.07 delay 1:30 @14:33:19.11 PSR.select :air @14:33:19.13 PSV.dial PSR @14:33:20.20 Pulling 0.5ml of PSR.wash @14:33:20.21 PSR.select :wash @14:33:20.33 PS.seek 0.5ml @14:33:26.31 delay 5 seconds @14:33:31.33 PSR.select :air @14:33:31.45 PS.seek 2ml @14:33:47.62 PTV.dial :puck @14:33:48.37 PSV.dial PTV @14:33:49.14 PBV.dial :waste1 @14:33:50.54 PS.seek 1.5ml @14:33:56.46 delay 3 seconds @14:33:59.48 PBV.dial between PSV and waste1 @14:33:59.85 PS.seek 0ml @14:34:16.44 delay 5:00 @14:39:16.46 recoverPSRreagent08 @14:39:16.47 PTV.dial :puck @14:39:16.55 PBV.dial :waste1 @14:39:16.93 delay 30 seconds @14:39:46.95 PBV.dial between PSV and waste1 @14:39:47.32 PSR.select :air @14:39:47.33 PSV.dial PSR @14:39:48.11 PS.reconfigure :fast @14:39:48.15 PS.seek 2.5ml @14:40:11.89 PSV.dial PTV @14:40:12.66 delay 5 seconds @14:40:17.68 PSV.dial PSR @14:40:18.46 delay 5 seconds @14:40:23.48 PSV.dial PTV @14:40:24.24 delay 5 seconds @14:40:29.26 PSV.dial :air @14:40:30.03 PS.seek 0ml @14:40:53.40 PTV.dial :PRVmixing,via: PRV @14:40:54.72 PSV.dial :mixloop @14:40:55.32 PRV.dial :ab1 @14:40:56.07 PS.reconfigure :slow @14:40:56.12 PS.seek 0.15ml @14:40:59.61 delay 2 seconds @14:41:01.63 CBV.dial PRV @14:41:01.66
PRV.dial CBV @14:41:02.23 PS.seek 0.35ml @14:41:06.83 delay 2 seconds @14:41:08.85 CBV.dial between PRV and PSV @14:41:09.17 PRV.dial :air @14:41:09.64 PS.seek 0.9ml @14:41:21.42 PRV.dial :waste2 @14:41:22.18 PS.seek 0.5ml @14:41:30.86 addPMLReagent08 @14:41:30.87 PSV.dial :air @14:41:31.32 PS.reconfigure :default @14:41:31.37 PS.seek 2ml @14:41:47.55 PSV.dial :PML @14:41:48.02 PTV.dial :mixing @14:41:48.82 PBV.dial :waste1 @14:41:49.25 PS.seek 1.85ml @14:41:51.55 PBV.dial between PSV and waste1 @14:41:51.93 PS.seek 0ml @14:42:11.83 delay 2 seconds @14:42:13.85 Beginning samplePassC @14:42:13.86 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:42:14.05 Elbow.reconfigure :tight @14:42:14.10 Elbow.seek FlushPuck::Garage @14:42:15.47 Elbow.reconfigure :loose @14:42:15.57 Forearm.seek :garage @14:42:16.61 Hand.close @14:42:17.29 Forearm.seek :retract @14:42:18.27 Elbow.reconfigure :tight @14:42:18.32 Elbow.seek CC @14:42:21.20 Elbow.reconfigure :loose @14:42:21.30 Forearm.reconfigure :tight @14:42:21.35 Forearm.seek CC @14:42:22.59 CC.seek :closed @14:42:27.12 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.47 @14:42:27.19 Hand.open @14:42:27.87 Forearm.reconfigure :loose @14:42:27.92 Forearm.seek :retract @14:42:28.89 PSV.dial PSR @14:42:29.96 Pulling 1.5ml of PSR.flush @14:42:29.97 PSR.select :flush @14:42:30.09 PS.seek 1.5ml @14:42:46.17 delay 5 seconds @14:42:51.19 PSV.dial :waste1 @14:42:51.66 PS.seek 0ml @14:43:07.77 PSV.dial PSR @14:43:08.24 PSR.select :air @14:43:08.35 PS.seek 2.5ml @14:43:34.77 PRV.dial CBV,avoiding: :ab2 @14:43:34.79
CBV.dial PRV @14:43:36.44 PSV.dial :mixloop @14:43:37.87 CTV.dial :air,avoiding: :intake @14:43:38.17 PTV.dial :PRVmixing @14:43:38.95 PS.seek 0ml @14:44:05.39 PSV.dial :air @14:44:05.87 PS.seek 2.5ml @14:44:32.26 PSV.dial :mixloop @14:44:32.76 PS.seek 0ml @14:44:59.08 PSV.dial :air @14:44:59.55 PS.seek 2.5ml @14:45:25.89 PSV.dial :mixloop @14:45:26.37 PS.seek 0ml @14:45:52.67 Garaging FlushPuck in Collection Clamp @14:45:52.74 Elbow.reconfigure :tight @14:45:52.79 Elbow.seek CC @14:45:53.06 Elbow.reconfigure :loose @14:45:53.16 Forearm.seek CC @14:45:54.11 Hand.close @14:45:54.67 CC.seek :open @14:45:59.32 Forearm.seek :retract @14:46:00.30 Elbow.reconfigure :tight @14:46:00.34 Elbow.seek :garage @14:46:03.32 Elbow.reconfigure :loose @14:46:03.42 Forearm.reconfigure :tight @14:46:03.47 Forearm.seek :garage @14:46:04.52 Hand.open @14:46:05.23 Forearm.reconfigure :loose @14:46:05.28 Forearm.seek :retract @14:46:06.27 delayUntil "14:50:13.84" @14:46:06.29 Whole Cell Archive sampling 1000ml @14:46:06.31 Loading wcr puck from tube 7 into Collection Clamp @14:46:06.56 SC.reconfigure :tight @14:46:06.61 SC.seek 7 @14:46:06.67 SC.configure :limp @14:46:07.33 Elbow.reconfigure :tight @14:46:07.39 Elbow.seek 7 @14:46:09.36 Forearm.reconfigure :tight @14:46:09.41 Forearm.seek 7 + 450 counts @14:46:10.42 SE.reconfigure :pick @14:46:10.48 SE.seek :beamBreak @14:46:12.50 SE.configure :default @14:46:12.66 Elbow.seek 7 @14:46:12.85 Forearm.seek 7 + 300 counts @14:46:13.04 Forearm.reconfigure :loose @14:46:13.09 Hand.close @14:46:13.69 SE.seek :down @14:46:13.89 Forearm.seek :retract @14:46:14.85 Elbow.seek CC @14:46:15.40 SE.stop @14:46:16.48 Elbow.reconfigure :loose @14:46:16.58 Forearm.reconfigure :tight @14:46:16.64 Forearm.seek CC @14:46:17.91 CC.seek :closed @14:46:22.46 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.29 @14:46:22.47 Hand.open @14:46:23.67 Forearm.reconfigure :loose @14:46:23.72 Forearm.seek :retract @14:46:24.69 collectSample 1000ml w/bubble point of 35psi @14:46:24.70 Priming 7ml @14:46:24.71 Intake.select :close @14:46:24.73 Exhaust.select :close @14:46:24.74 SS.hold @14:46:24.95 Calibrating pressure sensors @14:46:24.96 CTV.dial :air @14:46:25.05 SSV.dial :spit,avoiding: :exhaust @14:46:25.14 SS.reconfigure :fast @14:46:25.19 SS.seek 5ml @14:46:27.96 SSV.dial CBV @14:46:27.98 CBV.dial SSV @14:46:29.37 delay 3.8 seconds @14:46:33.30 Sensor limits: Intake max 99.5psia; Outlet max 91.6psia @14:46:33.31 SSV.dial :spit @14:46:34.65 SS.seek 0ml @14:46:37.41 SSV.dial CBV @14:46:38.75 CTV.dial :intake @14:46:39.55 SSV.dial CBV @14:46:39.57 CBV.dial SSV @14:46:39.65 Allowing 40s for 7.0ml of remaining 7.0ml @14:46:39.67 Intake.select :open @14:46:41.83 Intake @14.6psia, vacuum from 9.1 to 12.1psi @14:46:41.85 SS.reconfigure :inhaling 2.20ml/s @14:46:41.90 SS.seek 7ml @14:46:48.61 Stroke completed -- waiting for pressure to equalize ... @14:46:48.63 SS.reconfigure :exhale @14:46:48.67 SS.hold @14:47:11.16 Intake.select :close @14:47:11.32 SSV.dial :exhaust @14:47:12.08 Exhaust.select :open @14:47:12.20 SS.seek 0ml @14:47:16.48 Exhaust.select :close @14:47:16.59 Sampling 1000.0ml @14:47:16.61 Intake.select :close @14:47:16.62 Exhaust.select :close @14:47:16.63 SS.reconfigure :default @14:47:16.68 SS.hold @14:47:16.89 CTV.dial :intake @14:47:16.97 SSV.dial CBV @14:47:17.00 CBV.dial SSV @14:47:17.79 Allowing 130s for 25.0ml of remaining 1000.0ml @14:47:17.80 Intake.select :open @14:47:19.97 Intake @15.3psia, vacuum from 9.8 to 12.8psi @14:47:19.99 SS.reconfigure :inhaling 2.20ml/s @14:47:20.04 SS.seek 25ml @14:47:26.72 Vacuum too high -- waiting up to 40 seconds @14:47:26.73 SS.reconfigure :exhale @14:47:26.79 SS.hold @14:47:33.98 Intake @15.9psia, vacuum from 10.0 to 13.0psi @14:47:34.00 SS.reconfigure :inhaling 1.76ml/s @14:47:34.05 SS.seek 25ml @14:47:39.15 Vacuum too high -- waiting up to 40 seconds @14:47:39.17 SS.reconfigure :exhale @14:47:39.22 SS.hold @14:47:46.00 Intake @17.1psia, vacuum from 10.0 to 13.0psi @14:47:46.02 SS.reconfigure :inhaling 1.41ml/s @14:47:46.06 SS.seek 25ml @14:47:49.69 Vacuum too high -- waiting up to 40 seconds @14:47:49.71 SS.reconfigure :exhale @14:47:49.76 SS.hold @14:47:52.91 Intake @16.8psia, vacuum from 10.0 to 13.0psi @14:47:52.93 SS.reconfigure :inhaling 1.13ml/s @14:47:52.98 SS.seek 25ml @14:47:57.39 Stroke completed -- waiting for pressure to equalize ... @14:47:57.40 SS.reconfigure :exhale @14:47:57.45 SS.hold @14:48:26.52 Intake.select :close @14:48:26.68 SSV.dial :exhaust @14:48:27.44 Exhaust.select :open @14:48:27.56 SS.seek 0ml @14:48:36.63 Exhaust.select :close @14:48:36.74 SSV.dial CBV @14:48:36.77 CBV.dial SSV @14:48:37.55 Allowing 130s for 25.0ml of remaining 975.0ml @14:48:37.56 Intake.select :open @14:48:39.73 Intake @16.0psia, vacuum from 10.0 to 13.0psi @14:48:39.75 SS.reconfigure :inhaling 1.13ml/s @14:48:39.80 SS.seek 25ml @14:48:56.46 Vacuum too high -- waiting up to 40 seconds @14:48:56.48 SS.reconfigure :exhale @14:48:56.53 SS.hold @14:49:04.32 Intake @18.3psia, vacuum from 10.0 to 13.0psi @14:49:04.34 SS.reconfigure :inhaling 0.91ml/s @14:49:04.39 SS.seek 25ml @14:49:09.84 Vacuum too high -- waiting up to 40 seconds @14:49:09.85 SS.reconfigure :exhale @14:49:09.91 SS.hold @14:49:13.00 Intake @18.3psia, vacuum from 10.0 to 13.0psi @14:49:13.02 SS.reconfigure :inhaling 0.73ml/s @14:49:13.07 SS.seek 25ml @14:49:18.38 Stroke completed -- waiting for pressure to equalize ... @14:49:18.39 SS.reconfigure :exhale @14:49:18.44 SS.hold @14:49:49.36 Intake.select :close @14:49:49.52 SSV.dial :exhaust @14:49:50.28 Exhaust.select :open @14:49:50.40 SS.seek 0ml @14:49:59.23 Exhaust.select :close @14:49:59.35 SSV.dial CBV @14:49:59.83 CBV.dial SSV @14:50:00.63 Allowing 130s for 25.0ml of remaining 950.0ml @14:50:00.64 Intake.select :open @14:50:02.82 Intake @16.0psia, vacuum from 10.0 to 13.0psi @14:50:02.84 SS.reconfigure :inhaling 0.73ml/s @14:50:02.89 SS.seek 25ml @14:50:13.85 recoverPRVreagent08 @14:50:13.87 PTV.dial :mixing @14:50:14.63 PBV.dial :waste1 @14:50:15.01 delay 30 seconds @14:50:39.22 Stroke completed -- waiting for pressure to equalize ... @14:50:39.23 SS.reconfigure :exhale @14:50:39.28 SS.hold @14:50:45.03 PBV.dial between PSV and waste1 @14:50:45.40 PSV.dial :air @14:50:46.01 PS.reconfigure :fast @14:50:46.06 PS.seek 2.5ml @14:51:09.94 Intake.select :close @14:51:10.11 PSV.dial :mixloop @14:51:10.14 SSV.dial :exhaust @14:51:10.62 delay 2 seconds @14:51:10.95 Exhaust.select :open @14:51:11.07 SS.seek 0ml @14:51:12.64 PSV.dial :air @14:51:13.10 delay 2 seconds @14:51:15.12 PSV.dial :mixloop @14:51:15.60 delay 2 seconds @14:51:17.62 PSV.dial :air @14:51:18.24 PS.seek 0ml @14:51:20.17 Exhaust.select :close @14:51:20.28 SSV.dial CBV @14:51:20.30 CBV.dial SSV @14:51:21.12 Allowing 130s for 25.0ml of remaining 925.0ml @14:51:21.14 Intake.select :open @14:51:23.32 Intake @16.1psia, vacuum from 10.0 to 13.0psi @14:51:23.34 SS.reconfigure :inhaling 0.73ml/s @14:51:23.39 SS.seek 25ml @14:51:41.98 washCycleDA #1 of 2 @14:51:41.99 PSR.select :air @14:51:42.01 PSV.dial PSR @14:51:43.35 Pulling 0.5ml of PSR.wash @14:51:43.36 PSR.select :wash @14:51:43.48 PS.reconfigure :default @14:51:43.53 PS.seek 0.5ml @14:51:49.54 delay 5 seconds @14:51:54.56 PSR.select :air @14:51:54.68 PS.seek 2ml @14:51:59.40 Stroke completed -- waiting for pressure to equalize ... @14:51:59.42 SS.reconfigure :exhale @14:51:59.46 SS.hold @14:52:10.90 PTV.dial :puck @14:52:11.65 PSV.dial PTV @14:52:12.43 PBV.dial :waste1 @14:52:12.86 PS.seek 1.5ml @14:52:18.88 delay 3 seconds @14:52:21.90 PBV.dial between PSV and waste1 @14:52:22.28 PS.seek 0ml @14:52:28.32 Intake.select :close @14:52:28.94 SSV.dial :exhaust @14:52:29.70 Exhaust.select :open @14:52:29.82 SS.seek 0ml @14:52:38.38 delay 2:00 @14:52:38.64 Exhaust.select :close @14:52:38.75 SSV.dial CBV @14:52:38.80 CBV.dial SSV @14:52:39.56 Allowing 130s for 25.0ml of remaining 900.0ml @14:52:39.58 Intake.select :open @14:52:41.75 Intake @16.1psia, vacuum from 10.0 to 13.0psi @14:52:41.77 SS.reconfigure :inhaling 0.73ml/s @14:52:41.81 SS.seek 25ml @14:53:18.32 Stroke completed -- waiting for pressure to equalize ... @14:53:18.34 SS.reconfigure :exhale @14:53:18.39 SS.hold @14:53:47.44 Intake.select :close @14:53:47.60 SSV.dial :exhaust @14:53:48.38 Exhaust.select :open @14:53:48.50 SS.seek 0ml @14:53:57.51 Exhaust.select :close @14:53:57.62 SSV.dial CBV @14:53:57.64 CBV.dial SSV @14:53:58.43 Allowing 130s for 25.0ml of remaining 875.0ml @14:53:58.45 Intake.select :open @14:54:00.62 Intake @16.1psia, vacuum from 10.0 to 13.0psi @14:54:00.64 SS.reconfigure :inhaling 0.73ml/s @14:54:00.69 SS.seek 25ml @14:54:36.70 Stroke completed -- waiting for pressure to equalize ... @14:54:36.71 SS.reconfigure :exhale @14:54:36.76 SS.hold @14:54:38.42 recoverPSRreagent08 @14:54:38.43 PTV.dial :puck @14:54:38.50 PBV.dial :waste1 @14:54:38.88 delay 30 seconds @14:55:05.46 Intake.select :close @14:55:05.62 SSV.dial :exhaust @14:55:06.39 Exhaust.select :open @14:55:06.51 SS.seek 0ml @14:55:08.90 PBV.dial between PSV and waste1 @14:55:09.27 PSR.select :air @14:55:09.29 PSV.dial PSR @14:55:10.07 PS.reconfigure :fast @14:55:10.12 PS.seek 2.5ml @14:55:15.48 Exhaust.select :close @14:55:15.59 SSV.dial CBV @14:55:15.64 CBV.dial SSV @14:55:16.39 Allowing 130s for 25.0ml of remaining 850.0ml @14:55:16.40 Intake.select :open @14:55:18.58 Intake @16.1psia, vacuum from 10.0 to 13.0psi @14:55:18.60 SS.reconfigure :inhaling 0.73ml/s @14:55:18.65 SS.seek 25ml @14:55:34.10 PSV.dial PTV @14:55:35.32 delay 5 seconds @14:55:40.34 PSV.dial PSR @14:55:41.11 delay 5 seconds @14:55:44.55 Vacuum too high -- waiting up to 40 seconds @14:55:44.57 SS.reconfigure :exhale @14:55:44.62 SS.hold @14:55:46.13 PSV.dial PTV @14:55:47.02 delay 5 seconds @14:55:52.03 PSV.dial :air @14:55:52.26 Intake @16.0psia, vacuum from 10.0 to 13.0psi @14:55:52.28 SS.reconfigure :inhaling 0.59ml/s @14:55:52.32 SS.seek 25ml @14:55:52.82 PS.seek 0ml @14:56:05.12 Stroke completed -- waiting for pressure to equalize ... @14:56:05.13 SS.reconfigure :exhale @14:56:05.18 SS.hold @14:56:16.16 washCycleDA #2 of 2 @14:56:16.19 PSR.select :air @14:56:16.21 PSV.dial PSR @14:56:17.55 Pulling 0.5ml of PSR.wash @14:56:17.56 PSR.select :wash @14:56:17.67 PS.reconfigure :default @14:56:17.72 PS.seek 0.5ml @14:56:23.85 delay 5 seconds @14:56:27.81 Intake.select :close @14:56:27.97 SSV.dial :exhaust @14:56:28.74 Exhaust.select :open @14:56:28.86 SS.seek 0ml @14:56:28.91 PSR.select :air @14:56:29.04 PS.seek 2ml @14:56:37.70 Exhaust.select :close @14:56:37.81 SSV.dial CBV @14:56:37.84 CBV.dial SSV @14:56:38.62 Allowing 130s for 25.0ml of remaining 825.0ml @14:56:38.63 Intake.select :open @14:56:40.80 Intake @16.1psia, vacuum from 10.0 to 13.0psi @14:56:41.25 SS.reconfigure :inhaling 0.59ml/s @14:56:41.33 SS.seek 25ml @14:56:45.16 PTV.dial :puck @14:56:45.24 PSV.dial PTV @14:56:45.99 PBV.dial :waste1 @14:56:46.42 PS.seek 1.5ml @14:56:52.44 delay 3 seconds @14:56:55.46 PBV.dial between PSV and waste1 @14:56:55.84 PS.seek 0ml @14:57:11.91 delay 2:00 @14:57:26.30 Stroke completed -- waiting for pressure to equalize ... @14:57:26.32 SS.reconfigure :exhale @14:57:26.36 SS.hold @14:57:52.98 Intake.select :close @14:57:53.14 SSV.dial :exhaust @14:57:53.90 Exhaust.select :open @14:57:54.02 SS.seek 0ml @14:58:02.76 Exhaust.select :close @14:58:02.87 SSV.dial CBV @14:58:02.90 CBV.dial SSV @14:58:03.68 Allowing 130s for 25.0ml of remaining 800.0ml @14:58:03.69 Intake.select :open @14:58:05.86 Intake @16.1psia, vacuum from 10.0 to 13.0psi @14:58:05.88 SS.reconfigure :inhaling 0.59ml/s @14:58:05.93 SS.seek 25ml @14:58:41.76 Vacuum too high -- waiting up to 40 seconds @14:58:41.78 SS.reconfigure :exhale @14:58:41.84 SS.hold @14:58:54.71 Intake @16.9psia, vacuum from 10.0 to 13.0psi @14:58:54.73 SS.reconfigure :inhaling 0.47ml/s @14:58:54.77 SS.seek 25ml @14:59:06.29 Stroke completed -- waiting for pressure to equalize ... @14:59:06.30 SS.reconfigure :exhale @14:59:06.35 SS.hold @14:59:12.00 recoverPSRreagent08 @14:59:12.01 PTV.dial :puck @14:59:12.08 PBV.dial :waste1 @14:59:12.45 delay 30 seconds @14:59:26.78 Intake.select :close @14:59:26.94 SSV.dial :exhaust @14:59:27.73 Exhaust.select :open @14:59:27.84 SS.seek 0ml @14:59:36.66 Exhaust.select :close @14:59:36.78 SSV.dial CBV @14:59:36.80 CBV.dial SSV @14:59:37.60 Allowing 130s for 25.0ml of remaining 775.0ml @14:59:37.62 Intake.select :open @14:59:39.79 Intake @15.9psia, vacuum from 10.0 to 13.0psi @14:59:39.81 SS.reconfigure :inhaling 0.47ml/s @14:59:39.86 SS.seek 25ml @14:59:42.47 PBV.dial between PSV and waste1 @14:59:42.84 PSR.select :air @14:59:42.86 PSV.dial PSR @14:59:43.63 PS.reconfigure :fast @14:59:43.68 PS.seek 2.5ml @15:00:07.65 PSV.dial PTV @15:00:08.55 delay 5 seconds @15:00:13.57 PSV.dial PSR @15:00:14.31 delay 5 seconds @15:00:19.33 PSV.dial PTV @15:00:20.09 delay 5 seconds @15:00:25.12 PSV.dial :air @15:00:26.00 PS.seek 0ml @15:00:35.36 Stroke completed -- waiting for pressure to equalize ... @15:00:35.38 SS.reconfigure :exhale @15:00:35.42 SS.hold @15:00:49.28 getPMLReagent08 @15:00:49.29 PRV.dial :air @15:00:49.79 delay 5 seconds @15:00:54.81 PTV.dial :PRVmixing @15:00:56.14 delay 5 seconds @15:01:01.15 PRV.dial :ab2 @15:01:01.62 PTV.dial :PRVmixing @15:01:01.70 PSV.dial :mixloop @15:01:02.16 PS.reconfigure :default @15:01:02.21 PS.seek 0.3ml @15:01:06.18 delay 2 seconds @15:01:08.20 PRV.dial :air @15:01:08.73 PS.seek 0.55ml @15:01:10.24 Intake.select :close @15:01:10.40 SSV.dial :exhaust @15:01:11.63 Exhaust.select :open @15:01:11.75 SS.seek 0ml @15:01:12.12 addPMLReagent08 @15:01:12.14 PSV.dial :air @15:01:12.74 PS.seek 2ml @15:01:20.53 Exhaust.select :close @15:01:20.65 SSV.dial CBV @15:01:20.67 CBV.dial SSV @15:01:21.46 Allowing 130s for 25.0ml of remaining 750.0ml @15:01:21.47 Intake.select :open @15:01:23.64 Intake @16.0psia, vacuum from 10.0 to 13.0psi @15:01:23.66 SS.reconfigure :inhaling 0.47ml/s @15:01:23.71 SS.seek 25ml @15:01:28.31 PSV.dial :PML @15:01:28.76 PTV.dial :mixing @15:01:29.53 PBV.dial :waste1 @15:01:29.96 PS.seek 1.85ml @15:01:32.28 PBV.dial between PSV and waste1 @15:01:32.64 PS.seek 0ml @15:01:52.31 delay 2 seconds @15:01:54.33 delay 8:00 @15:02:08.24 Vacuum too high -- waiting up to 40 seconds @15:02:08.26 SS.reconfigure :exhale @15:02:08.31 SS.hold @15:02:24.75 Intake @16.6psia, vacuum from 10.0 to 13.0psi @15:02:24.76 SS.reconfigure :inhaling 0.38ml/s @15:02:24.81 SS.seek 25ml @15:02:38.77 Stroke completed -- waiting for pressure to equalize ... @15:02:38.78 SS.reconfigure :exhale @15:02:38.83 SS.hold @15:03:03.38 Intake.select :close @15:03:03.54 SSV.dial :exhaust @15:03:04.30 Exhaust.select :open @15:03:04.42 SS.seek 0ml @15:03:13.25 Exhaust.select :close @15:03:13.36 SSV.dial CBV @15:03:13.41 CBV.dial SSV @15:03:14.16 Allowing 130s for 25.0ml of remaining 725.0ml @15:03:14.17 Intake.select :open @15:03:16.34 Intake @15.9psia, vacuum from 10.0 to 13.0psi @15:03:16.36 SS.reconfigure :inhaling 0.38ml/s @15:03:16.41 SS.seek 25ml @15:04:20.63 Vacuum too high -- waiting up to 40 seconds @15:04:20.65 SS.reconfigure :exhale @15:04:20.70 SS.hold @15:04:41.29 Intake @16.6psia, vacuum from 10.0 to 13.0psi @15:04:41.31 SS.reconfigure :inhaling 0.31ml/s @15:04:41.35 SS.seek 25ml @15:04:46.97 Stroke completed -- waiting for pressure to equalize ... @15:04:46.98 SS.reconfigure :exhale @15:04:47.03 SS.hold @15:05:07.56 Intake.select :close @15:05:07.72 SSV.dial :exhaust @15:05:08.51 Exhaust.select :open @15:05:08.63 SS.seek 0ml @15:05:17.76 Exhaust.select :close @15:05:17.87 SSV.dial CBV @15:05:17.92 CBV.dial SSV @15:05:18.66 Allowing 130s for 25.0ml of remaining 700.0ml @15:05:18.67 Intake.select :open @15:05:20.84 Intake @15.9psia, vacuum from 10.0 to 13.0psi @15:05:20.86 SS.reconfigure :inhaling 0.31ml/s @15:05:20.91 SS.seek 25ml @15:06:48.03 Stroke completed -- waiting for pressure to equalize ... @15:06:48.04 SS.reconfigure :exhale @15:06:48.09 SS.hold @15:07:28.29 SS.reconfigure :default @15:07:28.34 SS.hold @15:07:28.41 Intake.select :close @15:07:28.58 ShallowSampler::Clogged in WCR -- Intake @16psia & Outlet @14psia won't equalize after 325.0ml @15:07:28.59 SSV.dial :exhaust @15:07:29.36 Exhaust.select :open @15:07:29.47 SS.reconfigure :exhale @15:07:29.52 SS.seek 0ml @15:07:38.39 Exhaust.select :close @15:07:38.50 Sampled 325.0ml @15:07:38.52 Drying puck before fixing for 5:00 @15:07:38.53 CBV.dial :waste2b @15:07:39.29 delay 1:00 @15:08:39.75 CSV.dial :air @15:08:40.25 CS.reconfigure :fast @15:08:40.30 CS.seek 10ml @15:09:04.84 Pumping wcr puck from tube 7 up to 35psi... @15:09:04.86 CBV.dial SSV @15:09:06.12 CBV.dial between CSV and waste2b @15:09:07.10 CSV.dial between air and CTV @15:09:07.42 CS.seek 7.127ml @15:09:14.88 CTV.dial CSV,avoiding: :air @15:09:14.92 CSV.dial CTV @15:09:15.70 CS.reconfigure :pressurize @15:09:15.75 CS.seek 0ml @15:09:28.76 CBV.dial :waste2b @15:09:29.11 CS.reconfigure :default @15:09:29.16 CS.seek 0ml @15:09:35.65 delay 1:00 @15:09:54.35 recoverPRVreagent08 @15:09:54.36 PTV.dial :mixing @15:09:54.44 PBV.dial :waste1 @15:09:54.82 delay 30 seconds @15:10:24.84 PBV.dial between PSV and waste1 @15:10:25.22 PSV.dial :air @15:10:25.70 PS.reconfigure :fast @15:10:25.75 PS.seek 2.5ml @15:10:35.67 CTV.dial :air,avoiding: :intake @15:10:37.01 Fix #1 of 2 (with RNAlater) for 2:30 @15:10:37.03 CSV.dial CSR @15:10:37.58 Pulling 2ml of CSR.RNAlater @15:10:37.60 CSR.select :RNAlater @15:10:37.72 CS.seek 2ml @15:10:43.74 delay 5 seconds @15:10:48.76 CSR.select :air @15:10:48.88 CS.reconfigure :fast @15:10:48.93 CS.seek 10ml @15:10:49.64 PSV.dial :mixloop @15:10:50.11 delay 2 seconds @15:10:52.13 PSV.dial :air @15:10:52.60 delay 2 seconds @15:10:54.61 PSV.dial :mixloop @15:10:55.08 delay 2 seconds @15:10:57.10 PSV.dial :air @15:10:57.57 PS.seek 0ml @15:11:08.62 Pumping wcr puck from tube 7 up to 35psi... @15:11:08.64 CBV.dial SSV @15:11:09.89 CBV.dial between CSV and waste2b @15:11:10.89 CSV.dial between air and CTV @15:11:11.48 CS.seek 7.079ml @15:11:19.00 CTV.dial CSV,avoiding: :air @15:11:19.02 CSV.dial CTV @15:11:20.40 CS.reconfigure :pressurize @15:11:20.45 CS.seek 0ml @15:11:20.96 washCycleDA #1 of 3 @15:11:20.97 PSR.select :air @15:11:20.98 PSV.dial PSR @15:11:22.33 Pulling 0.5ml of PSR.wash @15:11:22.34 PSR.select :wash @15:11:22.46 PS.reconfigure :default @15:11:22.51 PS.seek 0.5ml @15:11:28.45 delay 5 seconds @15:11:33.48 PSR.select :air @15:11:33.60 PS.seek 2ml @15:11:38.23 delay 2:30 @15:11:49.73 PTV.dial :puck @15:11:50.49 PSV.dial PTV @15:11:51.24 PBV.dial :waste1 @15:11:51.67 PS.seek 1.5ml @15:11:57.67 delay 3 seconds @15:12:00.69 PBV.dial between PSV and waste1 @15:12:01.07 PS.seek 0ml @15:12:17.24 delay 2:00 @15:14:08.25 CBV.dial :waste2b @15:14:08.59 CS.reconfigure :default @15:14:08.64 CS.seek 0ml @15:14:10.14 delay 1:00 @15:14:17.26 recoverPSRreagent08 @15:14:17.27 PTV.dial :puck @15:14:17.34 PBV.dial :waste1 @15:14:17.72 delay 30 seconds @15:14:47.74 PBV.dial between PSV and waste1 @15:14:48.13 PSR.select :air @15:14:48.15 PSV.dial PSR @15:14:48.91 PS.reconfigure :fast @15:14:48.96 PS.seek 2.5ml @15:15:10.17 CTV.dial :air,avoiding: :intake @15:15:11.49 Fix #2 of 2 (with RNAlater) for 2:30 @15:15:11.51 CSV.dial CSR @15:15:11.94 Pulling 2ml of CSR.RNAlater @15:15:11.95 CSR.select :RNAlater @15:15:12.07 CS.seek 2ml @15:15:12.86 PSV.dial PTV @15:15:13.62 delay 5 seconds @15:15:18.24 delay 5 seconds @15:15:18.64 PSV.dial PSR @15:15:19.51 delay 5 seconds @15:15:23.26 CSR.select :air @15:15:23.38 CS.reconfigure :fast @15:15:23.43 CS.seek 10ml @15:15:24.53 PSV.dial PTV @15:15:25.28 delay 5 seconds @15:15:30.30 PSV.dial :air @15:15:31.07 PS.seek 0ml @15:15:43.58 Pumping wcr puck from tube 7 up to 35psi... @15:15:43.60 CBV.dial SSV @15:15:44.87 CBV.dial between CSV and waste2b @15:15:45.84 CSV.dial between air and CTV @15:15:46.57 CS.seek 7.111ml @15:15:53.99 CTV.dial CSV,avoiding: :air @15:15:54.01 CSV.dial CTV @15:15:54.42 washCycleDA #2 of 3 @15:15:54.43 PSR.select :air @15:15:54.45 PSV.dial PSR @15:15:55.43 CS.reconfigure :pressurize @15:15:55.48 CS.seek 0ml @15:15:55.79 Pulling 0.5ml of PSR.wash @15:15:55.80 PSR.select :wash @15:15:55.92 PS.reconfigure :default @15:15:55.97 PS.seek 0.5ml @15:16:01.89 delay 5 seconds @15:16:06.91 PSR.select :air @15:16:07.03 PS.seek 2ml @15:16:09.20 delay 2:30 @15:16:23.26 PTV.dial :puck @15:16:23.28 PSV.dial PTV @15:16:24.03 PBV.dial :waste1 @15:16:24.46 PS.seek 1.5ml @15:16:30.36 delay 3 seconds @15:16:33.37 PBV.dial between PSV and waste1 @15:16:33.75 PS.seek 0ml @15:16:49.92 delay 2:00 @15:18:39.22 CBV.dial :waste2b @15:18:39.57 CS.reconfigure :default @15:18:39.62 CS.seek 0ml @15:18:45.48 delay 1:00 @15:18:49.94 recoverPSRreagent08 @15:18:49.95 PTV.dial :puck @15:18:49.97 PBV.dial :waste1 @15:18:50.34 delay 30 seconds @15:19:20.36 PBV.dial between PSV and waste1 @15:19:20.74 PSR.select :air @15:19:20.76 PSV.dial PSR @15:19:21.66 PS.reconfigure :fast @15:19:21.71 PS.seek 2.5ml @15:19:45.50 CTV.dial :air,avoiding: :intake @15:19:45.60 PSV.dial PTV @15:19:46.36 delay 5 seconds @15:19:51.38 PSV.dial PSR @15:19:52.15 delay 5 seconds @15:19:57.17 PSV.dial PTV @15:19:57.95 delay 5 seconds @15:20:02.97 PSV.dial :air @15:20:03.73 PS.seek 0ml @15:20:26.88 washCycleDA #3 of 3 @15:20:26.89 PSR.select :air @15:20:26.91 PSV.dial PSR @15:20:28.24 Pulling 0.5ml of PSR.wash @15:20:28.25 PSR.select :wash @15:20:28.37 PS.reconfigure :default @15:20:28.42 PS.seek 0.5ml @15:20:34.41 delay 5 seconds @15:20:39.43 PSR.select :air @15:20:39.56 PS.seek 2ml @15:20:55.72 PTV.dial :puck @15:20:55.74 PSV.dial PTV @15:20:56.49 PBV.dial :waste1 @15:20:56.93 PS.seek 1.5ml @15:21:02.88 delay 3 seconds @15:21:05.90 PBV.dial between PSV and waste1 @15:21:06.28 PS.seek 0ml @15:21:22.45 delay 2:00 @15:23:22.47 recoverPSRreagent08 @15:23:22.48 PTV.dial :puck @15:23:22.49 PBV.dial :waste1 @15:23:22.86 delay 30 seconds @15:23:52.89 PBV.dial between PSV and waste1 @15:23:53.27 PSR.select :air @15:23:53.29 PSV.dial PSR @15:23:54.06 PS.reconfigure :fast @15:23:54.11 PS.seek 2.5ml @15:24:17.75 PSV.dial PTV @15:24:18.52 delay 5 seconds @15:24:23.54 PSV.dial PSR @15:24:24.30 delay 5 seconds @15:24:29.32 PSV.dial PTV @15:24:30.08 delay 5 seconds @15:24:35.10 PSV.dial :air @15:24:35.87 PS.seek 0ml @15:24:59.03 Gate.power :camera,:ON @15:24:59.05 Mixing DA Substrates @15:24:59.06 PSR.select :air @15:24:59.07 PSV.dial PSR @15:25:00.49 Pulling 0.25ml of PSR.sub1 @15:25:00.51 PSR.select :sub1 @15:25:00.71 PS.reconfigure :default @15:25:00.83 PS.seek 0.25ml @15:25:04.20 delay 5 seconds @15:25:09.22 Pulling 0.25ml of PSR.sub2 @15:25:09.24 PSR.select :sub2 @15:25:09.35 PS.seek 0.5ml @15:25:12.78 delay 5 seconds @15:25:17.80 PSR.select :air @15:25:17.92 PS.seek 2ml @15:25:34.09 PTV.dial :puck @15:25:34.11 PSV.dial PTV @15:25:34.89 PBV.dial :waste1 @15:25:35.34 PS.seek 1.5ml @15:25:41.28 delay 3 seconds @15:25:44.30 PBV.dial between PSV and waste1 @15:25:44.70 PS.seek 0ml @15:26:01.03 delay 10 seconds @15:26:11.06 recoverPSRreagent08 @15:26:11.07 PTV.dial :puck @15:26:11.08 PBV.dial :waste1 @15:26:11.45 delay 30 seconds @15:26:41.48 PBV.dial between PSV and waste1 @15:26:41.86 PSR.select :air @15:26:42.33 PSV.dial PSR @15:26:43.15 PS.reconfigure :fast @15:26:43.20 PS.seek 2.5ml @15:27:07.28 PSV.dial PTV @15:27:08.17 delay 5 seconds @15:27:13.19 PSV.dial PSR @15:27:13.95 delay 5 seconds @15:27:18.98 PSV.dial PTV @15:27:19.74 delay 5 seconds @15:27:24.76 PSV.dial :air @15:27:25.55 PS.seek 0ml @15:27:49.35 Additional air clear @15:27:49.36 PSR.select :air @15:27:49.37 PSV.dial PSR @15:27:50.73 Pulling 2ml of PSR.air @15:27:50.74 PSR.select :air @15:27:50.77 PS.reconfigure :default @15:27:50.81 PS.seek 2ml @15:28:12.03 delay 5 seconds @15:28:17.05 PTV.dial :puck @15:28:17.06 PSV.dial PTV @15:28:17.83 PBV.dial :waste1 @15:28:18.26 PS.seek 1.5ml @15:28:24.12 delay 3 seconds @15:28:27.14 PBV.dial between PSV and waste1 @15:28:27.52 delay 1 second @15:28:28.53 PSV.dial :waste1 @15:28:29.62 PS.seek 0ml @15:28:45.74 Processing Puck Heater at 28.1C before imaging @15:28:45.75 PH.coast @15:28:45.87 Imaging da puck in Processing Clamp while storing it to tube 6 @15:28:46.04 SC.reconfigure :tight @15:28:46.09 SC.seek 6 @15:28:49.66 SC.configure :limp @15:28:49.88 Elbow.reconfigure :tight @15:28:49.93 Elbow.seek 6 @15:28:51.69 Forearm.reconfigure :tight @15:28:51.74 Forearm.seek 6 + 450 counts @15:28:52.77 SE.reconfigure :pick @15:28:52.83 SE.seek :beamBreak @15:28:55.15 SE.configure :default @15:28:55.31 Elbow.reconfigure :limp @15:28:55.36 Forearm.reconfigure :loose @15:28:55.41 Forearm.seek 6 @15:28:55.68 Elbow.hold @15:28:55.75 Elbow.reconfigure :tight @15:28:55.85 SE.seek 12.11 Pucks below Top Plate @15:28:56.52 Forearm.force -20 @15:28:56.57 Forearm.seek 6 + 311 counts @15:28:56.87 Forearm.reconfigure :tight @15:28:56.92 Forearm.seek 6 + 450 counts @15:28:57.11 SE.seek 13.11 Pucks below Top Plate @15:28:57.73 Forearm.reconfigure :loose @15:28:57.77 Forearm.seek :retract @15:28:58.70 Elbow.seek PC @15:29:01.22 Elbow.reconfigure :loose @15:29:01.32 Forearm.seek PC @15:29:02.31 Hand.close @15:29:02.89 PC.seek :open @15:29:07.68 Forearm.seek :retract @15:29:08.66 Elbow.reconfigure :tight @15:29:08.71 Elbow.seek 6 @15:29:11.33 Forearm.reconfigure :tight @15:29:11.38 Forearm.seek 6 + 450 counts @15:29:12.43 Hand.open @15:29:13.07 SE.seek 12.76 Pucks below Top Plate @15:29:13.35 SE.reconfigure :seal @15:29:13.40 SE.seek 11.8 Pucks below Top Plate @15:29:13.93 Lights, camera, ACTION! @15:29:13.97 PuckCamera: snap -bin=2,2 -auto /var/log/esp/da23dec2015h750ml.tif @15:29:33.45 Waiting >20 seconds for da to relinquish FlushPuck @15:29:35.43 SX HX9/16 (USB:32): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 18.204 seconds /var/log/esp/da23dec2015h750ml.tif: TIFF Upload Complete @15:29:35.45 PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da23dec2015h750ml15s.tif @15:29:51.07 SX HX9/16 (USB:32): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15 seconds /var/log/esp/midres/da23dec2015h750ml15s.tif: TIFF Upload Complete @15:29:51.09 PuckCamera: snap -bin=2,2 20.0 /var/log/esp/midres/da23dec2015h750ml20s.tif @15:30:11.75 SX HX9/16 (USB:32): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 20 seconds /var/log/esp/midres/da23dec2015h750ml20s.tif: TIFF Upload Complete @15:30:11.76 Gate.power :camera,:OFF @15:30:11.78 DAfans.email "18.204 sec auto-exposure",Subject: "da23dec2015h750ml images!" @15:30:12.31 Elbow.seek 6 @15:30:12.56 Forearm.seek 6 + 450 counts @15:30:12.71 SE.reconfigure :gently @15:30:12.76 SE.seek 12.52 Pucks below Top Plate @15:30:13.86 Elbow.reconfigure :limp @15:30:13.93 Forearm.reconfigure :loose @15:30:13.98 Forearm.seek 6 @15:30:14.26 Elbow.hold @15:30:14.35 Elbow.reconfigure :tight @15:30:14.40 SE.reconfigure :default @15:30:14.47 SE.seek 12.7 Pucks below Top Plate @15:30:14.87 Forearm.force -20 @15:30:14.92 Forearm.seek 6 + 281 counts @15:30:15.21 Forearm.reconfigure :tight @15:30:15.26 Forearm.seek 6 + 450 counts @15:30:15.63 SE.seek :down @15:30:16.94 Forearm.reconfigure :loose @15:30:17.04 Forearm.seek :retract @15:30:17.55 SE.stop @15:30:18.22 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @15:30:18.81 Elbow.seek FlushPuck::Garage @15:30:20.91 Elbow.reconfigure :loose @15:30:21.13 Forearm.seek :garage @15:30:22.18 Hand.close @15:30:23.05 Forearm.seek :retract @15:30:24.05 Elbow.reconfigure :tight @15:30:24.15 Elbow.seek PC @15:30:25.48 Elbow.reconfigure :loose @15:30:25.68 Forearm.reconfigure :tight @15:30:25.79 Forearm.seek PC @15:30:28.48 PC.seek :closed @15:30:33.37 Processing Clamp onSpring/offSpring for 15/25 samples, on/off torque 1.66 @15:30:33.51 Hand.open @15:30:34.28 Forearm.reconfigure :loose @15:30:34.36 Forearm.seek :retract @15:30:35.38 PSR.select :air @15:30:35.45 Storing wcr puck in Collection Clamp to tube 6 @15:30:35.48 PSV.dial PSR @15:30:36.01 SC.reconfigure :tight @15:30:36.14 SC.seek 6 @15:30:36.19 Pulling 0.5ml of PSR.flush @15:30:36.23 PSR.select :flush @15:30:36.48 SC.configure :limp @15:30:36.79 PS.seek 0.5ml @15:30:37.07 Elbow.reconfigure :tight @15:30:37.17 Elbow.seek 6 @15:30:40.00 Forearm.reconfigure :tight @15:30:40.09 Forearm.seek 6 + 450 counts @15:30:41.25 SE.reconfigure :pick @15:30:41.36 SE.seek :beamBreak @15:30:42.71 delay 5 seconds @15:30:43.87 SE.configure :default @15:30:44.20 Elbow.reconfigure :limp @15:30:44.31 Forearm.reconfigure :loose @15:30:44.40 Forearm.seek 6 @15:30:44.74 Elbow.hold @15:30:44.91 Elbow.reconfigure :tight @15:30:45.13 SE.seek 13.1 Pucks below Top Plate @15:30:46.75 Forearm.force -20 @15:30:46.85 Forearm.seek 6 + 278 counts @15:30:47.31 Forearm.reconfigure :tight @15:30:47.41 Forearm.seek 6 + 450 counts @15:30:47.69 SE.seek 14.1 Pucks below Top Plate @15:30:47.81 PSR.select :air @15:30:47.98 PS.seek 2.5ml @15:30:48.60 Forearm.reconfigure :loose @15:30:48.71 Forearm.seek :retract @15:30:49.75 Elbow.seek CC @15:30:49.95 upsync @15:30:51.42 Elbow.reconfigure :loose @15:30:51.53 Forearm.seek CC @15:30:52.52 Hand.close @15:30:53.14 CC.seek :open @15:30:57.90 Forearm.seek :retract @15:30:58.86 Elbow.reconfigure :tight @15:30:58.91 Elbow.seek 6 @15:31:00.66 Elbow.reconfigure :limp @15:31:00.71 Forearm.seek 6 @15:31:01.70 Elbow.hold @15:31:01.76 Elbow.reconfigure :tight @15:31:01.82 Hand.open @15:31:02.51 Forearm.force -20 @15:31:02.55 Forearm.seek 6 + 298 counts @15:31:03.31 Forearm.reconfigure :tight @15:31:03.36 Forearm.seek 6 + 450 counts @15:31:03.63 SE.seek :down @15:31:04.76 Forearm.reconfigure :loose @15:31:04.82 Forearm.seek :retract @15:31:05.38 SE.stop @15:31:05.76
Starting finalPurge @15:31:09.21 delay 1 second @15:31:10.23 PRV.dial :waste2,avoiding: :ab2 @15:31:12.10 PTV.dial :PRVmixing @15:31:13.43 PSV.dial :mixloop @15:31:14.60 PS.seek :empty @15:31:25.55
Waiting >20 seconds for psFlush to relinquish FlushPuck @15:31:40.99 PSR.select :air @15:31:41.01 PSV.dial PSR @15:31:42.06 Pulling 0.5ml of PSR.flush @15:31:42.07 PSR.select :flush @15:31:42.19 PS.seek 0.5ml @15:31:48.09 delay 5 seconds @15:31:53.11 PSR.select :air @15:31:53.23 PS.seek 2.5ml @15:32:14.56 delay 1 second @15:32:15.58 PBV.dial :waste1 @15:32:15.95 PTV.dial :mixing @15:32:16.71 PSV.dial :mixloop @15:32:17.78 PS.seek :empty @15:32:44.31 PSV.dial PSR @15:32:45.35 PSR.select :flush @15:32:45.48 PS.seek 1ml @15:32:56.59 PSR.select :air @15:32:56.71 PS.seek 2.5ml @15:33:12.87 PSV.dial PTV @15:33:13.63 PTV.dial :puck @15:33:14.37 PBV.dial :waste1 @15:33:14.45 PS.seek 0ml @15:33:40.99 PSV.dial PSR @15:33:41.74 PSR.select :flush @15:33:41.87 PS.seek 0.5ml @15:33:47.83 PSR.select :air @15:33:47.95 PS.seek 2.5ml @15:34:09.27 PSV.dial PTV @15:34:10.04 PBV.dial PSV @15:34:10.61 PS.seek 0ml @15:34:36.93 PSV.dial PBV @15:34:37.84 PS.seek 2.5ml @15:35:04.15 PSV.dial :waste1 @15:35:04.89 PS.seek 0ml @15:35:31.43 PSV.dial PSR @15:35:31.90 PSR.select :air @15:35:31.92 PS.seek 2.5ml @15:35:58.24 PSV.dial PTV @15:35:59.10 PBV.dial PSV @15:35:59.18 PS.seek 0ml @15:36:25.58 PSV.dial PBV @15:36:26.06 PS.seek 2.5ml @15:36:52.33 PSV.dial :waste1 @15:36:53.07 PS.seek 0ml @15:37:19.46 PSV.dial PSR @15:37:19.93 PSR.select :flush @15:37:20.05 PS.seek 1ml @15:37:31.27 PSR.select :air @15:37:31.38 PS.seek 2.5ml @15:37:47.58 PSV.dial PTV @15:37:48.32 PTV.dial :puck @15:37:48.39 PBV.dial :waste1 @15:37:48.96 PS.seek 0ml @15:38:15.30 PSV.dial PSR @15:38:16.05 PSR.select :flush @15:38:16.18 PS.seek 0.5ml @15:38:22.17 PSR.select :air @15:38:22.29 PS.seek 2.5ml @15:38:43.58 PSV.dial PTV @15:38:44.35 PBV.dial PSV @15:38:44.91 PS.seek 0ml @15:39:11.23 PSV.dial PBV @15:39:11.71 PS.seek 2.5ml @15:39:38.09 PSV.dial :waste1 @15:39:38.82 PS.seek 0ml @15:40:05.23 PSV.dial PSR @15:40:05.70 PSR.select :air @15:40:05.73 PS.seek 2.5ml @15:40:32.01 PSV.dial PTV @15:40:32.87 PBV.dial PSV @15:40:33.07 PS.seek 0ml @15:40:59.48 PSV.dial PBV @15:40:59.94 PS.seek 2.5ml @15:41:26.33 PSV.dial :waste1 @15:41:27.51 PS.seek 0ml @15:41:53.86
Loading FlushPuck into Collection Clamp from Processing Clamp @15:41:54.05 Elbow.seek PC @15:41:56.58 Elbow.reconfigure :loose @15:41:56.68 Forearm.seek PC @15:41:57.61 Hand.close @15:41:58.26 PC.seek :open @15:42:02.98 Forearm.seek :retract @15:42:03.93 Elbow.reconfigure :tight @15:42:03.97 Elbow.seek CC @15:42:07.62 Elbow.reconfigure :loose @15:42:07.72 Forearm.reconfigure :tight @15:42:07.77 Forearm.seek CC @15:42:09.04 CC.seek :closed @15:42:13.56 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.47 @15:42:13.63 Hand.open @15:42:14.31 Forearm.reconfigure :loose @15:42:14.36 Forearm.seek :retract @15:42:15.32 Flushing the Collection Valve::Manifold @15:42:15.44 CSV.dial CSR @15:42:15.90 Pulling 5ml of CSR.flush @15:42:15.91 CSR.select :flush @15:42:16.03 CS.seek 5ml @15:42:29.62 delay 5 seconds @15:42:34.63 CSR.select :air @15:42:34.80 CS.reconfigure :fast @15:42:34.85 CS.seek 7.501ml @15:42:41.68 CSV.dial :waste @15:42:43.40 CS.reconfigure :default @15:42:43.47 CS.seek 0ml @15:43:03.52 Flushing lower manifold's waste1 port @15:43:03.65 CSV.dial :flush @15:43:04.11 CS.reconfigure :fast @15:43:04.16 CS.seek 5ml @15:43:17.12 delay 5 seconds @15:43:22.14 CSV.dial :air @15:43:22.64 CS.seek 7ml @15:43:28.15 CSV.dial CTV @15:43:28.17
CTV.dial CSV @15:43:29.55 CBV.dial :waste1 @15:43:30.32 CS.seek 0ml @15:43:47.08 Flushing lower manifold's waste2 port @15:43:47.21 CSV.dial :flush @15:43:47.95 CS.seek 5ml @15:44:00.86 delay 5 seconds @15:44:05.88 CSV.dial :air @15:44:06.41 CS.seek 7ml @15:44:12.02 CSV.dial CTV @15:44:12.05
CTV.dial CSV @15:44:12.52 CBV.dial :waste2 @15:44:13.57 CS.seek 0ml @15:44:30.27 Flushing lower manifold's waste2b port @15:44:30.40 CSV.dial :flush @15:44:31.14 CS.seek 5ml @15:44:44.08 delay 5 seconds @15:44:49.10 CSV.dial :air @15:44:49.63 CS.seek 7ml @15:44:55.24 CSV.dial CTV @15:44:55.26
CTV.dial CSV @15:44:55.71 CBV.dial :waste2b @15:44:56.76 CS.seek 0ml @15:45:13.27 Disinfecting external Intake with kill @15:45:13.40 CSV.dial CSR @15:45:13.96 Pulling 7.999ml of CSR.kill @15:45:13.97 CSR.select :kill @15:45:14.09 CS.seek 8ml @15:45:33.93 delay 5 seconds @15:45:38.94 CSR.select :air @15:45:39.06 CS.seek 10ml @15:45:44.59 CSV.dial CBV @15:45:44.61
CBV.dial CSV @15:45:45.69 CTV.dial :intake @15:45:46.46 Intake.select :open @15:45:46.57 CS.seek 2ml @15:46:06.06 Intake.select :close @15:46:06.16 CTV.dial :air @15:46:06.93 CS.reconfigure :default @15:46:06.98 CS.seek 0ml @15:46:12.92 CSV.dial CSR @15:46:13.94 Pulling 5ml of CSR.flush @15:46:13.95 CSR.select :flush @15:46:14.07 CS.seek 5ml @15:46:27.67 delay 5 seconds @15:46:32.69 CSR.select :air @15:46:32.81 CS.seek 10ml @15:46:46.49 CSV.dial CBV @15:46:47.62 CS.seek 0ml @15:47:13.93 Garaging FlushPuck in Collection Clamp @15:47:14.00 Elbow.reconfigure :tight @15:47:14.04 Elbow.seek CC @15:47:14.32 Elbow.reconfigure :loose @15:47:14.42 Forearm.seek CC @15:47:15.45 Hand.close @15:47:16.13 CC.seek :open @15:47:20.79 Forearm.seek :retract @15:47:21.80 Elbow.reconfigure :tight @15:47:21.84 Elbow.seek :garage @15:47:24.82 Elbow.reconfigure :loose @15:47:24.92 Forearm.reconfigure :tight @15:47:24.97 Forearm.seek :garage @15:47:26.00 Hand.open @15:47:26.68 Forearm.reconfigure :loose @15:47:26.73 Forearm.seek :retract @15:47:27.72 Completed [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] begun 13:20:22 @15:47:27.77 Asleep after completing DA_WCR_122023 assays @15:47:27.79 GoodNews.email "{1 => 33,\n 2 => 33,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 15,\n 7 => 16}\nCTD data unavailable\nCan@15:39:32, 30% flow, 17.2C, 35% humidity, 15.1psia, 13.734V, 0.345A, 264.718Ah, 4.73W\nSampled 325.0ml",Subject: "Asleep after completing DA_WCR_122023 assays" @15:47:32.32 upsync + ESPwhich DA_WCR_122123.rb + fn=DA_WCR_122123.rb + IFS=: + set /home/esp/esp2/mission . + fullPath=/home/esp/esp2/mission/DA_WCR_122123.rb + [ -f /home/esp/esp2/mission/DA_WCR_122123.rb ] + realpath /home/esp/esp2/mission/DA_WCR_122123.rb + return 0 + scriptPath=/home/esp/esp2local/mission/DA_WCR_122123.rb + exec ruby /home/esp/esp2/lib/esp.rb /home/esp/esp2local/mission/DA_WCR_122123.rb @11:15:45.47UTC21-Dec-23
ESP2 v3.42 I2C Gateway v3.20 for core bus @11:15:48.12 Configuration for short puck jake 1/11/21 by brent@mbari.org /home/esp/esp2/type/shallow/jake/configure.rb @11:15:51.75 Polling Can every 10:00 @11:15:52.01 Cleanly exited at 15:47:32UTC20-Dec-23 @11:15:52.06 Loading /home/esp/esp2local/mission/DA_WCR_122123.rb mission @11:15:52.12 GoodNews.email "Starting DA_WCR_122123 w/pucks in tube 7\n{1 => 33,\n 2 => 33,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 15,\n 7 => 16}\nCTD data unavailable\nCan@11:15:51, 32% flow, 15.3C, 37% humidity, 15.0psia, 13.739V, 0.284A, 270.067Ah, 3.91W",Subject: "DA_WCR_122123 begun" @11:15:52.15
Preparing to sleep until + 00:00:05 @11:15:52.20 Asleep til + 00:00:05 @11:15:52.21 Continuing at 11:15 @11:15:52.50 Gate.power :core,:OFF @11:15:52.51 Gate.power :raw,:OFF @11:15:52.52 Shutdown @11:15:55.60 GoodNews.email "Then, in tube 7: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_122123.rb:14\n{1 => 33,\n 2 => 33,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 15,\n 7 => 16}\nCTD data unavailable\nCan@11:15:52, 32% flow, 15.3C, 37% humidity, 15.0psia, 13.739V, 0.290A, 270.067Ah, 3.99W",Subject: "Asleep until 11:15" @11:15:56.99 upsync @11:15:57.10
Powering core bus @11:16:00.57 upsync @11:16:02.62
Gate.power :raw,:ON @11:16:03.34 CS.configure :default @11:16:03.39 PS.configure :default @11:16:03.43 SS.configure :default @11:16:03.48 CC.configure :default @11:16:03.53 PC.configure :default @11:16:03.60 Forearm.configure :loose @11:16:03.65 Elbow.configure :loose @11:16:03.72 SC.configure :loose @11:16:03.76 SE.configure :default @11:16:03.81 CH.configure :default @11:16:03.84 PH.configure :default @11:16:03.88 All dwarf controllers running firmware version 3.85 @11:16:03.99 Valve.reset @11:16:04.21 Intake.select :close @11:16:04.24 Exhaust.select :close @11:16:04.30 CTV.dial :air @11:16:04.39 PSV.dial :air @11:16:04.54
Forearm.home.seek :retract @11:16:04.60 CSV.dial :waste1 @11:16:05.07 SSV.dial :spit,avoiding: :exhaust @11:16:05.33 Hand.close @11:16:05.37
Elbow.reconfigure :loose @11:16:05.45 Elbow.jog 1500 @11:16:05.52 PS.home.seek 0ml @11:16:05.97
Elbow.reconfigure :loose @11:16:05.99 CS.reconfigure :fast @11:16:06.04 SS.home.seek 0ml @11:16:06.13
Elbow.home.seek :clear @11:16:06.21 CS.home.seek 0ml @11:16:09.51
SE.home.seek :down @11:16:10.10 SC.reconfigure :tight @11:16:10.17 SC.home.seek :initial @11:16:13.94 PS.stop @11:16:35.42
SC.configure :limp @11:16:35.48 Forearm.seek :retract @11:16:35.55 Elbow.seek :hammer @11:16:36.61 Forearm.reconfigure :tight @11:16:36.65 Forearm.seek retracted @11:16:37.33 PC.home.seek :open @11:16:37.36
CC.home.seek :open @11:16:40.74 Phase [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] @11:16:40.76 Notice.email "At 11:16:40 began in tube 7: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_122123.rb:14\n{1 => 33,\n 2 => 33,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 15,\n 7 => 16}\nCTD data unavailable\nCan@11:16:40, 32% flow, 15.3C, 37% humidity, 15.0psia, 13.730V, 0.309A, 270.072Ah, 4.24W",Subject: "Started DA_WCR_122123 phase initialPurge; da [1000, 1000] #da and wsr are run simultanelously" @11:16:40.78
Starting initialPurge @11:16:40.80 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @11:16:40.87 Hand.open @11:16:41.66 Elbow.reconfigure :tight @11:16:41.71 Elbow.seek FlushPuck::Garage @11:16:43.66 Elbow.reconfigure :loose @11:16:43.76 Forearm.reconfigure :loose @11:16:43.81 Forearm.seek :garage @11:16:44.80 Hand.close @11:16:45.55 Forearm.seek :retract @11:16:46.15 upsync @11:16:46.60
Elbow.reconfigure :tight @11:16:46.64 Elbow.seek CC @11:16:49.70 Elbow.reconfigure :loose @11:16:49.80 Forearm.reconfigure :tight @11:16:49.85 Forearm.seek CC @11:16:51.13 CC.seek :closed @11:16:55.75 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.42 @11:16:55.82 Hand.open @11:16:56.48 Forearm.reconfigure :loose @11:16:56.53 Forearm.seek :retract @11:16:57.50 Flushing the Collection Valve::Manifold @11:16:57.63 CSV.dial CSR @11:16:59.02 Pulling 5ml of CSR.flush @11:16:59.03 CSR.select :flush @11:16:59.15 CS.reconfigure :default @11:16:59.62 CS.seek 5ml @11:17:13.27 delay 5 seconds @11:17:18.29 CSR.select :air @11:17:18.46 CS.reconfigure :fast @11:17:18.51 CS.seek 7.501ml @11:17:25.34 CSV.dial :waste @11:17:26.62 CS.reconfigure :default @11:17:26.67 CS.seek 0ml @11:17:46.90 Purging external Intake @11:17:47.02 CSV.dial :flush @11:17:47.48 CS.reconfigure :fast @11:17:47.53 CS.seek 10ml @11:18:11.84 delay 5 seconds @11:18:16.87 CSV.dial CBV @11:18:16.90
CBV.dial CSV @11:18:17.63 CTV.dial :intake @11:18:18.40 Intake.select :open @11:18:18.51 CS.seek 0ml @11:18:41.99 Intake.select :close @11:18:42.09 CTV.dial :air @11:18:42.88 Flushing upper manifold @11:18:43.01 CSV.dial :flush @11:18:43.75 CS.seek 5ml @11:18:56.58 delay 5 seconds @11:19:01.61 CSV.dial :air @11:19:02.13 CS.seek 7ml @11:19:07.68 CSV.dial CBV @11:19:07.70
CBV.dial CSV @11:19:08.76 CTV.dial :air @11:19:08.84 CS.seek 0ml @11:19:25.46 delay 7 seconds @11:19:32.49 CS.seek 3ml @11:19:40.40 CSV.dial :waste @11:19:40.86 CS.seek 0ml @11:19:48.60 CSV.dial :flush @11:19:49.06 CS.seek 3ml @11:19:57.12 delay 5 seconds @11:20:02.13 CSV.dial :air @11:20:02.63 CS.seek 5ml @11:20:08.21 CSV.dial :air @11:20:08.29 CS.seek 0ml @11:20:20.65 delay 7 seconds @11:20:27.67 CS.seek 3ml @11:20:35.71 CSV.dial :waste @11:20:36.45 CS.seek 0ml @11:20:44.09 Garaging FlushPuck in Collection Clamp @11:20:44.16 Elbow.reconfigure :tight @11:20:44.20 Elbow.seek CC @11:20:44.51 Elbow.reconfigure :loose @11:20:44.61 Forearm.seek CC @11:20:45.66 Hand.close @11:20:46.29 CC.seek :open @11:20:51.36 Forearm.seek :retract @11:20:52.35 Elbow.reconfigure :tight @11:20:52.40 Elbow.seek :garage @11:20:55.36 Elbow.reconfigure :loose @11:20:55.47 Forearm.reconfigure :tight @11:20:55.52 Forearm.seek :garage @11:20:56.54 Hand.open @11:20:57.22 Forearm.reconfigure :loose @11:20:57.27 Forearm.seek :retract @11:20:58.25 da sampling at most 1000ml, wcr at most 1000ml @11:20:58.28 Loading sh1 puck from tube 7 into Collection Clamp @11:20:58.30 DAfans.email "Started da in tube 7 at 11:20:58UTC21-Dec-23\n{1 => 33,\n 2 => 33,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 15,\n 7 => 16}\nCTD data unavailable\nCan@11:16:40, 32% flow, 15.3C, 37% humidity, 15.0psia, 13.730V, 0.309A, 270.072Ah, 4.24W",Subject: "da sampling at most 1000ml, wcr at most 1000ml" @11:20:58.58
SC.reconfigure :tight @11:20:58.64 SC.seek 7 @11:21:02.76 upsync @11:21:08.51
SC.configure :limp @11:21:08.74 Elbow.reconfigure :tight @11:21:08.79 Elbow.seek 7 @11:21:10.81 Forearm.reconfigure :tight @11:21:10.86 Forearm.seek 7 + 450 counts @11:21:11.88 SE.reconfigure :pick @11:21:11.94 SE.seek :beamBreak @11:21:14.01 SE.configure :default @11:21:14.17 Elbow.seek 7 @11:21:14.41 Forearm.seek 7 + 300 counts @11:21:14.62 Forearm.reconfigure :loose @11:21:14.66 Hand.close @11:21:15.30 SE.seek :down @11:21:15.88
Forearm.seek :retract @11:21:16.90 Elbow.seek CC @11:21:17.09 SE.stop @11:21:18.52
Elbow.reconfigure :loose @11:21:18.62 Forearm.reconfigure :tight @11:21:18.67 Forearm.seek CC @11:21:19.97 CC.seek :closed @11:21:24.55 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.31 @11:21:24.56 Hand.open @11:21:25.22 Forearm.reconfigure :loose @11:21:25.27 Forearm.seek :retract @11:21:26.23 collectSample 1000ml w/bubble point of 24psi @11:21:26.24 Priming 7ml @11:21:26.25 Intake.select :close @11:21:26.26 Exhaust.select :close @11:21:26.27 SS.hold @11:21:26.48 Calibrating pressure and temperature sensors @11:21:26.51 CTV.dial :air @11:21:26.59 SSV.dial :spit,avoiding: :exhaust @11:21:26.66 SS.reconfigure :fast @11:21:26.71 SS.seek 5ml @11:21:29.81 SSV.dial CBV @11:21:29.84
CBV.dial SSV @11:21:31.17 delay 3.8 seconds @11:21:35.10 Sensor limits: Intake max 98.3psia; Outlet max 91.6psia SSV.dial :spit @11:21:36.43 SS.seek 0ml @11:21:39.03 SSV.dial CBV @11:21:40.34 CTV.dial :intake @11:21:41.13 SSV.dial CBV @11:21:41.15
CBV.dial SSV @11:21:41.24 Allowing 40s for 7.0ml of remaining 7.0ml @11:21:41.25 Intake.select :open @11:21:43.41 Intake @14.7psia, vacuum from 9.2 to 12.2psi @11:21:43.43 SS.reconfigure :inhaling 2.20ml/s @11:21:43.48 SS.seek 7ml @11:21:50.03 Stroke completed -- waiting for pressure to equalize ... @11:21:50.04 SS.reconfigure :exhale @11:21:50.08 SS.hold @11:21:57.00 Intake.select :close @11:21:57.15 SSV.dial :exhaust @11:21:57.92 Exhaust.select :open @11:21:58.03 SS.seek 0ml @11:22:02.29 Exhaust.select :close @11:22:02.40 Sampling 1000.0ml @11:22:02.41 Intake.select :close @11:22:02.42 Exhaust.select :close @11:22:02.44 SS.reconfigure :default @11:22:02.48 SS.hold @11:22:02.68 CTV.dial :intake @11:22:02.87 SSV.dial CBV @11:22:02.89
CBV.dial SSV @11:22:03.68 Allowing 130s for 25.0ml of remaining 1000.0ml @11:22:03.70 Intake.select :open @11:22:05.86 Intake @15.2psia, vacuum from 9.7 to 12.7psi @11:22:05.87 SS.reconfigure :inhaling 2.20ml/s @11:22:05.92 SS.seek 25ml @11:22:13.74 Vacuum too high -- waiting up to 40 seconds @11:22:13.75 SS.reconfigure :exhale @11:22:13.80 SS.hold @11:22:19.80 Intake @17.4psia, vacuum from 10.0 to 13.0psi @11:22:19.82 SS.reconfigure :inhaling 1.76ml/s @11:22:20.29 SS.seek 25ml @11:22:23.97 Vacuum too high -- waiting up to 40 seconds @11:22:23.98 SS.reconfigure :exhale @11:22:24.03 SS.hold @11:22:26.76 Intake @17.6psia, vacuum from 10.0 to 13.0psi @11:22:26.78 SS.reconfigure :inhaling 1.41ml/s @11:22:26.83 SS.seek 25ml @11:22:30.36 Vacuum too high -- waiting up to 40 seconds @11:22:30.38 SS.reconfigure :exhale @11:22:30.43 SS.hold @11:22:32.59 Intake @17.6psia, vacuum from 10.0 to 13.0psi @11:22:32.61 SS.reconfigure :inhaling 1.13ml/s @11:22:32.66 SS.seek 25ml @11:22:37.51 Stroke completed -- waiting for pressure to equalize ... @11:22:37.52 SS.reconfigure :exhale @11:22:37.57 SS.hold @11:22:56.02 Intake.select :close @11:22:56.17 SSV.dial :exhaust @11:22:56.93 Exhaust.select :open @11:22:57.05 SS.seek 0ml @11:23:05.79 Exhaust.select :close @11:23:05.90 SSV.dial CBV @11:23:05.92
CBV.dial SSV @11:23:06.73 Allowing 130s for 25.0ml of remaining 975.0ml @11:23:06.74 Intake.select :open @11:23:08.91 Intake @15.8psia, vacuum from 10.0 to 13.0psi @11:23:08.92 SS.reconfigure :inhaling 1.13ml/s @11:23:08.97 SS.seek 25ml @11:23:33.65 Stroke completed -- waiting for pressure to equalize ... @11:23:33.66 SS.reconfigure :exhale @11:23:33.71 SS.hold @11:23:58.09 Intake.select :close @11:23:58.24 SSV.dial :exhaust @11:23:58.98 Exhaust.select :open @11:23:59.09 SS.seek 0ml @11:24:07.87 Exhaust.select :close @11:24:07.98 SSV.dial CBV @11:24:08.00
CBV.dial SSV @11:24:08.79 Allowing 130s for 25.0ml of remaining 950.0ml @11:24:08.80 Intake.select :open @11:24:10.97 Intake @16.0psia, vacuum from 10.0 to 13.0psi @11:24:10.98 SS.reconfigure :inhaling 1.13ml/s @11:24:11.03 SS.seek 25ml @11:24:32.77 Vacuum too high -- waiting up to 40 seconds @11:24:32.78 SS.reconfigure :exhale @11:24:32.83 SS.hold @11:24:38.55 Intake @16.9psia, vacuum from 10.0 to 13.0psi @11:24:38.57 SS.reconfigure :inhaling 0.91ml/s @11:24:38.62 SS.seek 25ml @11:24:42.63 Stroke completed -- waiting for pressure to equalize ... @11:24:42.64 SS.reconfigure :exhale @11:24:42.69 SS.hold @11:25:03.51 Intake.select :close @11:25:03.66 SSV.dial :exhaust @11:25:04.42 Exhaust.select :open @11:25:04.54 SS.seek 0ml @11:25:13.23 Exhaust.select :close @11:25:13.33 SSV.dial CBV @11:25:13.35
CBV.dial SSV @11:25:14.13 Allowing 130s for 25.0ml of remaining 925.0ml @11:25:14.14 Intake.select :open @11:25:16.31 Intake @16.1psia, vacuum from 10.0 to 13.0psi @11:25:16.33 SS.reconfigure :inhaling 0.91ml/s @11:25:16.38 SS.seek 25ml @11:25:46.43 Stroke completed -- waiting for pressure to equalize ... @11:25:46.44 SS.reconfigure :exhale @11:25:46.48 SS.hold @11:26:08.82 Intake.select :close @11:26:08.98 SSV.dial :exhaust @11:26:09.75 Exhaust.select :open @11:26:09.86 SS.seek 0ml @11:26:18.79 Exhaust.select :close @11:26:18.90 SSV.dial CBV @11:26:18.92
CBV.dial SSV @11:26:19.70 Allowing 130s for 25.0ml of remaining 900.0ml @11:26:19.71 Intake.select :open @11:26:21.87 Intake @16.1psia, vacuum from 10.0 to 13.0psi @11:26:21.89 SS.reconfigure :inhaling 0.91ml/s @11:26:21.94 SS.seek 25ml @11:26:51.32 Stroke completed -- waiting for pressure to equalize ... @11:26:51.33 SS.reconfigure :exhale @11:26:51.37 SS.hold @11:27:14.22 Intake.select :close @11:27:14.38 SSV.dial :exhaust @11:27:15.13 Exhaust.select :open @11:27:15.25 SS.seek 0ml @11:27:24.07 Exhaust.select :close @11:27:24.18 SSV.dial CBV @11:27:24.20
CBV.dial SSV @11:27:24.98 Allowing 130s for 25.0ml of remaining 875.0ml @11:27:24.99 Intake.select :open @11:27:27.15 Intake @16.1psia, vacuum from 10.0 to 13.0psi @11:27:27.17 SS.reconfigure :inhaling 0.91ml/s @11:27:27.22 SS.seek 25ml @11:27:56.61 Stroke completed -- waiting for pressure to equalize ... @11:27:56.62 SS.reconfigure :exhale @11:27:56.67 SS.hold @11:28:17.12 Intake.select :close @11:28:17.27 SSV.dial :exhaust @11:28:18.04 Exhaust.select :open @11:28:18.16 SS.seek 0ml @11:28:26.88 Exhaust.select :close @11:28:26.99 SSV.dial CBV @11:28:27.01
CBV.dial SSV @11:28:27.80 Allowing 130s for 25.0ml of remaining 850.0ml @11:28:27.81 Intake.select :open @11:28:29.98 Intake @16.2psia, vacuum from 10.0 to 13.0psi @11:28:29.99 SS.reconfigure :inhaling 0.91ml/s @11:28:30.04 SS.seek 25ml @11:28:51.92 Vacuum too high -- waiting up to 40 seconds @11:28:51.93 SS.reconfigure :exhale @11:28:51.98 SS.hold @11:28:56.94 Intake @16.0psia, vacuum from 10.0 to 13.0psi @11:28:56.96 SS.reconfigure :inhaling 0.73ml/s @11:28:57.00 SS.seek 25ml @11:29:07.00 Stroke completed -- waiting for pressure to equalize ... @11:29:07.01 SS.reconfigure :exhale @11:29:07.06 SS.hold @11:29:21.65 Intake.select :close @11:29:21.81 SSV.dial :exhaust @11:29:22.59 Exhaust.select :open @11:29:22.71 SS.seek 0ml @11:29:31.52 Exhaust.select :close @11:29:31.63 SSV.dial CBV @11:29:31.65
CBV.dial SSV @11:29:32.43 Allowing 130s for 25.0ml of remaining 825.0ml @11:29:32.44 Intake.select :open @11:29:34.60 Intake @16.2psia, vacuum from 10.0 to 13.0psi @11:29:34.61 SS.reconfigure :inhaling 0.73ml/s @11:29:34.66 SS.seek 25ml @11:30:10.52 Stroke completed -- waiting for pressure to equalize ... @11:30:10.53 SS.reconfigure :exhale @11:30:10.57 SS.hold @11:30:28.93 Intake.select :close @11:30:29.09 SSV.dial :exhaust @11:30:29.85 Exhaust.select :open @11:30:29.96 SS.seek 0ml @11:30:38.67 Exhaust.select :close @11:30:38.78 SSV.dial CBV @11:30:38.81
CBV.dial SSV @11:30:39.58 Allowing 130s for 25.0ml of remaining 800.0ml @11:30:39.59 Intake.select :open @11:30:41.76 Intake @16.1psia, vacuum from 10.0 to 13.0psi @11:30:41.78 SS.reconfigure :inhaling 0.73ml/s @11:30:41.82 SS.seek 25ml @11:31:18.08 Stroke completed -- waiting for pressure to equalize ... @11:31:18.09 SS.reconfigure :exhale @11:31:18.14 SS.hold @11:31:36.55 Intake.select :close @11:31:36.71 SSV.dial :exhaust @11:31:37.47 Exhaust.select :open @11:31:37.58 SS.seek 0ml @11:31:46.24 Exhaust.select :close @11:31:46.35 SSV.dial CBV @11:31:46.37
CBV.dial SSV @11:31:47.16 Allowing 130s for 25.0ml of remaining 775.0ml @11:31:47.17 Intake.select :open @11:31:49.34 Intake @16.1psia, vacuum from 10.0 to 13.0psi @11:31:49.35 SS.reconfigure :inhaling 0.73ml/s @11:31:49.40 SS.seek 25ml @11:32:22.66 Vacuum too high -- waiting up to 40 seconds @11:32:22.68 SS.reconfigure :exhale @11:32:22.73 SS.hold @11:32:32.61 Intake @16.9psia, vacuum from 10.0 to 13.0psi @11:32:32.62 SS.reconfigure :inhaling 0.59ml/s @11:32:32.67 SS.seek 25ml @11:32:37.01 Stroke completed -- waiting for pressure to equalize ... @11:32:37.02 SS.reconfigure :exhale @11:32:37.07 SS.hold @11:32:48.46 Intake.select :close @11:32:48.62 SSV.dial :exhaust @11:32:49.38 Exhaust.select :open @11:32:49.49 SS.seek 0ml @11:32:58.20 Exhaust.select :close @11:32:58.31 SSV.dial CBV @11:32:58.33
CBV.dial SSV @11:32:59.13 Allowing 130s for 25.0ml of remaining 750.0ml @11:32:59.14 Intake.select :open @11:33:01.30 Intake @16.1psia, vacuum from 10.0 to 13.0psi @11:33:01.32 SS.reconfigure :inhaling 0.59ml/s @11:33:01.37 SS.seek 25ml @11:33:46.01 Stroke completed -- waiting for pressure to equalize ... @11:33:46.02 SS.reconfigure :exhale @11:33:46.07 SS.hold @11:34:04.49 Intake.select :close @11:34:04.65 SSV.dial :exhaust @11:34:05.41 Exhaust.select :open @11:34:05.52 SS.seek 0ml @11:34:14.25 Exhaust.select :close @11:34:14.35 SSV.dial CBV @11:34:14.38
CBV.dial SSV @11:34:15.15 Allowing 130s for 25.0ml of remaining 725.0ml @11:34:15.17 Intake.select :open @11:34:17.33 Intake @16.2psia, vacuum from 10.0 to 13.0psi @11:34:17.35 SS.reconfigure :inhaling 0.59ml/s @11:34:17.40 SS.seek 25ml @11:35:02.23 Stroke completed -- waiting for pressure to equalize ... @11:35:02.24 SS.reconfigure :exhale @11:35:02.29 SS.hold @11:35:24.70 Intake.select :close @11:35:24.85 SSV.dial :exhaust @11:35:25.61 Exhaust.select :open @11:35:25.72 SS.seek 0ml @11:35:34.51 Exhaust.select :close @11:35:34.62 SSV.dial CBV @11:35:34.65
CBV.dial SSV @11:35:35.42 Allowing 130s for 25.0ml of remaining 700.0ml @11:35:35.43 Intake.select :open @11:35:37.60 Intake @16.1psia, vacuum from 10.0 to 13.0psi @11:35:37.61 SS.reconfigure :inhaling 0.59ml/s @11:35:37.66 SS.seek 25ml @11:36:22.53 Stroke completed -- waiting for pressure to equalize ... @11:36:22.54 SS.reconfigure :exhale @11:36:22.58 SS.hold @11:36:47.07 Intake.select :close @11:36:47.22 SSV.dial :exhaust @11:36:47.98 Exhaust.select :open @11:36:48.09 SS.seek 0ml @11:36:56.85 Exhaust.select :close @11:36:56.96 SSV.dial CBV @11:36:56.98
CBV.dial SSV @11:36:57.78 Allowing 130s for 25.0ml of remaining 675.0ml @11:36:57.79 Intake.select :open @11:36:59.95 Intake @16.0psia, vacuum from 10.0 to 13.0psi @11:36:59.96 SS.reconfigure :inhaling 0.59ml/s @11:37:00.01 SS.seek 25ml @11:37:39.13 Vacuum too high -- waiting up to 40 seconds @11:37:39.14 SS.reconfigure :exhale @11:37:39.19 SS.hold @11:37:52.04 Intake @16.5psia, vacuum from 10.0 to 13.0psi @11:37:52.05 SS.reconfigure :inhaling 0.47ml/s @11:37:52.10 SS.seek 25ml @11:37:59.38 Stroke completed -- waiting for pressure to equalize ... @11:37:59.39 SS.reconfigure :exhale @11:37:59.44 SS.hold @11:38:14.43 Intake.select :close @11:38:14.58 SSV.dial :exhaust @11:38:15.34 Exhaust.select :open @11:38:15.45 SS.seek 0ml @11:38:24.32 Exhaust.select :close @11:38:24.43 SSV.dial CBV @11:38:24.45
CBV.dial SSV @11:38:25.23 Allowing 130s for 25.0ml of remaining 650.0ml @11:38:25.24 Intake.select :open @11:38:27.40 Intake @16.1psia, vacuum from 10.0 to 13.0psi @11:38:27.42 SS.reconfigure :inhaling 0.47ml/s @11:38:27.47 SS.seek 25ml @11:39:22.47 Stroke completed -- waiting for pressure to equalize ... @11:39:22.48 SS.reconfigure :exhale @11:39:22.53 SS.hold @11:39:49.31 Intake.select :close @11:39:49.47 SSV.dial :exhaust @11:39:50.23 Exhaust.select :open @11:39:50.35 SS.seek 0ml @11:39:59.07 Exhaust.select :close @11:39:59.18 SSV.dial CBV @11:39:59.20
CBV.dial SSV @11:39:59.98 Allowing 130s for 25.0ml of remaining 625.0ml @11:39:59.99 Intake.select :open @11:40:02.15 Intake @16.1psia, vacuum from 10.0 to 13.0psi @11:40:02.16 SS.reconfigure :inhaling 0.47ml/s @11:40:02.21 SS.seek 25ml @11:40:57.85 Stroke completed -- waiting for pressure to equalize ... @11:40:57.86 SS.reconfigure :exhale @11:40:57.90 SS.hold @11:41:26.56 Intake.select :close @11:41:26.71 SSV.dial :exhaust @11:41:27.48 Exhaust.select :open @11:41:27.59 SS.seek 0ml @11:41:36.22 Exhaust.select :close @11:41:36.34 SSV.dial CBV @11:41:36.38
CBV.dial SSV @11:41:37.12 Allowing 130s for 25.0ml of remaining 600.0ml @11:41:37.13 Intake.select :open @11:41:39.29 Intake @16.0psia, vacuum from 10.0 to 13.0psi @11:41:39.31 SS.reconfigure :inhaling 0.47ml/s @11:41:39.35 SS.seek 25ml @11:42:31.68 Vacuum too high -- waiting up to 40 seconds @11:42:31.70 SS.reconfigure :exhale @11:42:31.75 SS.hold @11:42:48.41 Intake @16.6psia, vacuum from 10.0 to 13.0psi @11:42:48.43 SS.reconfigure :inhaling 0.38ml/s @11:42:48.48 SS.seek 25ml @11:42:53.02 Stroke completed -- waiting for pressure to equalize ... @11:42:53.03 SS.reconfigure :exhale @11:42:53.07 SS.hold @11:43:11.33 Intake.select :close @11:43:11.48 SSV.dial :exhaust @11:43:12.24 Exhaust.select :open @11:43:12.36 SS.seek 0ml @11:43:21.11 Exhaust.select :close @11:43:21.22 SSV.dial CBV @11:43:21.24
CBV.dial SSV @11:43:22.05 Allowing 130s for 25.0ml of remaining 575.0ml @11:43:22.06 Intake.select :open @11:43:24.23 Intake @16.0psia, vacuum from 10.0 to 13.0psi @11:43:24.24 SS.reconfigure :inhaling 0.38ml/s @11:43:24.29 SS.seek 25ml @11:44:33.02 Stroke completed -- waiting for pressure to equalize ... @11:44:33.03 SS.reconfigure :exhale @11:44:33.07 SS.hold @11:45:01.99 Intake.select :close @11:45:02.14 SSV.dial :exhaust @11:45:02.89 Exhaust.select :open @11:45:03.01 SS.seek 0ml @11:45:12.02 Exhaust.select :close @11:45:12.12 SSV.dial CBV @11:45:12.14
CBV.dial SSV @11:45:12.95 Allowing 130s for 25.0ml of remaining 550.0ml @11:45:12.97 Intake.select :open @11:45:15.13 Intake @16.2psia, vacuum from 10.0 to 13.0psi @11:45:15.15 SS.reconfigure :inhaling 0.38ml/s @11:45:15.20 SS.seek 25ml @11:46:23.25 Stroke completed -- waiting for pressure to equalize ... @11:46:23.26 SS.reconfigure :exhale @11:46:23.31 SS.hold @11:47:02.03 Intake.select :close @11:47:02.18 SSV.dial :exhaust @11:47:02.94 Exhaust.select :open @11:47:03.05 SS.seek 0ml @11:47:11.84 Exhaust.select :close @11:47:11.96 SSV.dial CBV @11:47:11.98
CBV.dial SSV @11:47:12.76 Allowing 130s for 25.0ml of remaining 525.0ml @11:47:12.78 Intake.select :open @11:47:14.94 Intake @16.1psia, vacuum from 10.0 to 13.0psi @11:47:14.96 SS.reconfigure :inhaling 0.38ml/s @11:47:15.01 SS.seek 25ml @11:48:12.64 Vacuum too high -- waiting up to 40 seconds @11:48:12.66 SS.reconfigure :exhale @11:48:12.71 SS.hold @11:48:29.01 Intake @16.0psia, vacuum from 10.0 to 13.0psi @11:48:29.03 SS.reconfigure :inhaling 0.31ml/s @11:48:29.07 SS.seek 25ml @11:48:43.23 Stroke completed -- waiting for pressure to equalize ... @11:48:43.24 SS.reconfigure :exhale @11:48:43.29 SS.hold @11:49:07.84 Intake.select :close @11:49:08.00 SSV.dial :exhaust @11:49:08.76 Exhaust.select :open @11:49:08.88 SS.seek 0ml @11:49:17.67 Exhaust.select :close @11:49:17.78 SSV.dial CBV @11:49:17.80
CBV.dial SSV @11:49:18.57 Allowing 130s for 25.0ml of remaining 500.0ml @11:49:18.59 Intake.select :open @11:49:20.75 Intake @16.1psia, vacuum from 10.0 to 13.0psi @11:49:20.76 SS.reconfigure :inhaling 0.31ml/s @11:49:20.81 SS.seek 25ml @11:50:48.43 Stroke completed -- waiting for pressure to equalize ... @11:50:48.44 SS.reconfigure :exhale @11:50:48.49 SS.hold @11:51:23.62 Intake.select :close @11:51:23.77 SSV.dial :exhaust @11:51:24.53 Exhaust.select :open @11:51:24.64 SS.seek 0ml @11:51:33.37 Exhaust.select :close @11:51:33.48 SSV.dial CBV @11:51:33.50
CBV.dial SSV @11:51:34.29 Allowing 130s for 25.0ml of remaining 475.0ml @11:51:34.30 Intake.select :open @11:51:36.47 Intake @16.1psia, vacuum from 10.0 to 13.0psi @11:51:36.48 SS.reconfigure :inhaling 0.31ml/s @11:51:36.53 SS.seek 25ml @11:53:04.18 Stroke completed -- waiting for pressure to equalize ... @11:53:04.19 SS.reconfigure :exhale @11:53:04.24 SS.hold @11:53:44.40 SS.reconfigure :default @11:53:44.46 SS.hold @11:53:44.53 Intake.select :close @11:53:44.69 ShallowSampler::Clogged in MAIN -- Filtering too Slowly after 550.0ml @11:53:44.70 SSV.dial :exhaust @11:53:45.46 Exhaust.select :open @11:53:45.58 SS.reconfigure :exhale @11:53:45.62 SS.seek 0ml @11:53:54.48 Exhaust.select :close @11:53:54.58 Sampled 550.0ml @11:53:54.60 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @11:53:54.61 Intake.select :close @11:53:54.67 CSV.dial :waste @11:53:54.84 SSV.dial CBV,avoiding: :exhaust @11:53:54.86 CS.seek 0ml @11:53:54.89
CBV.dial SSV @11:53:55.05 CSV.dial :air @11:53:55.78 CS.seek 10ml @11:53:56.79
CTV.dial :air @11:53:57.57 delay 15 seconds @11:54:12.59 SSV.dial :spit,avoiding: :exhaust @11:54:13.91 CTV.dial CSV,avoiding: :intake @11:54:13.94
SS.seek 0ml @11:54:14.06 SSV.dial CBV,avoiding: :exhaust @11:54:20.37 SSV.dial between CBV and plug @11:54:20.86 CBV.dial between SSV and waste2 @11:54:21.25 CSV.dial between air and CTV @11:54:21.58 CS.seek 8.444ml @11:54:26.02 CTV.dial CSV,avoiding: :air @11:54:26.05
CSV.dial CTV @11:54:26.40 CS.reconfigure :pressurize @11:54:26.45 CS.seek 0ml @11:54:40.59 BubblePoint: Intake @24.3psia, Outlet @0.6psia @11:54:40.60 CBV.dial SSV @11:54:40.93 SS.reconfigure :default @11:54:40.98 SS.seek 10ml @11:54:46.55 delay 15 seconds @11:55:01.68 Evacuated: Intake @23.8psia, Outlet @23.4psia @11:55:01.69 SSV.dial :spit,avoiding: :exhaust @11:55:02.73 SS.reconfigure :exhale @11:55:02.78 SS.seek 0ml @11:55:07.75 SS.reconfigure :fast @11:55:07.80 SS.seek 5ml @11:55:10.58 SSV.dial CBV,avoiding: :exhaust @11:55:10.59
CBV.dial SSV @11:55:11.95 CTV.dial :air,avoiding: :intake @11:55:13.29 CSV.dial CTV @11:55:13.32
SS.seek 10ml @11:55:14.52 CSV.dial :air @11:55:14.96 CS.reconfigure :fast @11:55:15.01 CS.seek 0ml @11:55:15.82
SSV.dial :spit,avoiding: :exhaust @11:55:17.14 SS.reconfigure :exhale @11:55:17.18 SS.seek 0ml @11:55:22.17 SS.reconfigure :fast @11:55:22.22 SS.seek 5ml @11:55:23.02 CS.stop @11:55:25.01 SSV.dial CBV,avoiding: :exhaust @11:55:25.03
CBV.dial SSV @11:55:27.36 SSV.dial :spit,avoiding: :exhaust @11:55:28.67 SS.reconfigure :default @11:55:28.71 SS.seek 0ml @11:55:31.94 SS.stop @11:55:31.98 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @11:55:31.99 Intake.select :close @11:55:32.06 CSV.dial :waste @11:55:32.19 SSV.dial CBV,avoiding: :exhaust @11:55:32.21
CBV.dial SSV @11:55:32.85 CS.seek 0ml @11:55:32.92 CSV.dial :air @11:55:33.56
CTV.dial :air @11:55:33.63 delay 15 seconds @11:55:34.09 CS.seek 10ml @11:55:48.65
SSV.dial :spit,avoiding: :exhaust @11:55:49.97 CTV.dial CSV,avoiding: :intake @11:55:50.00
SS.reconfigure :exhale @11:55:50.10 SS.seek 0ml @11:55:50.49 SSV.dial CBV,avoiding: :exhaust @11:55:58.46 SSV.dial between CBV and plug @11:55:58.95 CBV.dial between SSV and waste2 @11:55:59.33 CSV.dial between air and CTV @11:55:59.65 CS.seek 7.395ml @11:56:06.46 CTV.dial CSV,avoiding: :air @11:56:06.48
CSV.dial CTV @11:56:06.84 CS.reconfigure :pressurize @11:56:06.89 CS.seek 0ml @11:56:19.65 BubblePoint: Intake @24.3psia, Outlet @3.5psia @11:56:19.66 CBV.dial SSV @11:56:19.99 SS.reconfigure :default @11:56:20.04 SS.seek 10ml @11:56:25.65 delay 15 seconds @11:56:40.77 Evacuated: Intake @23.9psia, Outlet @23.7psia @11:56:40.78 SSV.dial :spit,avoiding: :exhaust @11:56:41.82 SS.reconfigure :exhale @11:56:41.87 SS.seek 0ml @11:56:46.84 SS.reconfigure :fast @11:56:46.89 SS.seek 5ml @11:56:49.71 SSV.dial CBV,avoiding: :exhaust @11:56:49.74
CBV.dial SSV @11:56:51.10 CTV.dial :air,avoiding: :intake @11:56:52.44 CSV.dial CTV @11:56:52.48
SS.seek 10ml @11:56:53.68 CSV.dial :air @11:56:54.13 CS.reconfigure :fast @11:56:54.18 CS.seek 0ml @11:56:54.99
SSV.dial :spit,avoiding: :exhaust @11:56:56.35 SS.reconfigure :exhale @11:56:56.40 SS.seek 0ml @11:57:00.98 CS.stop @11:57:01.39
SS.reconfigure :fast @11:57:01.44 SS.seek 5ml @11:57:04.40 SSV.dial CBV,avoiding: :exhaust @11:57:04.42
CBV.dial SSV @11:57:06.75 SSV.dial :spit,avoiding: :exhaust @11:57:08.49 SS.reconfigure :default @11:57:08.54 SS.seek 0ml @11:57:11.64 SS.stop @11:57:11.68 Lysing: Adding 2.0ml AqMeOH @11:57:11.70 CBV.dial between CSV and waste1,avoiding: :DBV @11:57:13.21 CTV.dial CSV @11:57:14.53 CSV.dial CSR @11:57:15.45 Pulling 1ml of CSR.AqMeOH CSR.select :AqMeOH @11:57:15.57 CS.reconfigure :default @11:57:15.61 CS.seek 1ml @11:57:19.14 delay 5 seconds @11:57:24.16 CSR.select :air @11:57:24.33 CS.seek 6.001ml @11:57:38.02 delay 2 seconds @11:57:40.03 CSV.dial :waste2 @11:57:40.51 CS.reconfigure :fast @11:57:40.56 CS.seek 0ml @11:57:54.95 CSV.dial CSR @11:57:55.41 Pulling 2ml of CSR.AqMeOH @11:57:55.42 CSR.select :AqMeOH @11:57:55.53 CS.reconfigure :default @11:57:55.58 CS.seek 2ml @11:58:01.74 delay 5 seconds @11:58:06.75 CSR.select :air @11:58:06.92 CS.seek 5ml @11:58:15.42 delay 2 seconds @11:58:17.44 CSV.dial CTV @11:58:17.90 CS.seek 1ml @11:58:29.28 Before lysing Collection Puck Heater at 12.9C/30.8psia @11:58:29.30 Heating Puck.from 7, :sh1 in Collection Clamp to 70C for 10:00 @11:58:29.31 CH.seek 70C,8:00 @12:00:50.42 CH.hold 10:00,70C @12:00:50.62 Begun lysing Collection Puck Heater at 68.1C/37.7psia @12:10:50.63 Ended lysing Collection Puck Heater at 70C/36.7psia @12:10:50.65 CS.seek 0ml @12:10:54.57 CTV.dial between spare and air,avoiding: :intake @12:10:55.61 CSV.dial :air @12:10:56.04 CS.seek 5ml @12:11:09.63 CSV.dial CTV @12:11:10.08 delay 2 seconds @12:11:12.10 CSV.dial :air @12:11:12.55 delay 2 seconds @12:11:14.57 CSV.dial CTV @12:11:15.02 delay 2 seconds @12:11:17.04 CSV.dial :air @12:11:17.49 delay 2 seconds @12:11:19.51 CS.seek 0ml @12:11:33.15 Puck @79.1psia after final lysis pressurization @12:11:33.16 Recovering Lysate into Collection syringe @12:11:33.17 CSV.dial CBV @12:11:34.19 CBV.dial CSV @12:11:34.58 CS.seek 5ml @12:11:48.44 delay 5 seconds @12:11:53.46 CTV.dial :air @12:11:53.99 CS.seek 7ml @12:11:59.88 delay 5 seconds @12:12:04.90 CSV.dial :air2 @12:12:05.34 CS.seek 10ml @12:12:13.91 CSV.dial between air and CTV,avoiding: CBV @12:12:15.07 Lysing Complete @12:12:15.14 Storing sh1 puck in Collection Clamp to tube 6 @12:12:15.31 SC.reconfigure :tight @12:12:15.36 SC.seek 6 @12:12:18.92 SC.configure :limp @12:12:19.14 Elbow.reconfigure :tight @12:12:19.18 Elbow.seek 6 @12:12:20.96 Forearm.reconfigure :tight @12:12:21.01 Forearm.seek 6 + 450 counts @12:12:22.03 SE.reconfigure :pick @12:12:22.09 SE.seek :beamBreak @12:12:24.27 SE.configure :default @12:12:24.43 Elbow.reconfigure :limp @12:12:24.48 Forearm.reconfigure :loose @12:12:24.52 Forearm.seek 6 @12:12:24.79 Elbow.hold @12:12:25.30 Elbow.reconfigure :tight @12:12:25.40 SE.seek 14.11 Pucks below Top Plate @12:12:25.92 Forearm.force -20 @12:12:25.96 Forearm.seek 6 + 284 counts @12:12:26.24 Forearm.reconfigure :tight @12:12:26.29 Forearm.seek 6 + 450 counts @12:12:26.47 SE.seek 15.11 Pucks below Top Plate @12:12:27.02 Forearm.reconfigure :loose @12:12:27.07 Forearm.seek :retract @12:12:28.03 Elbow.seek CC @12:12:29.73 Elbow.reconfigure :loose @12:12:29.83 Forearm.seek CC @12:12:30.81 Hand.close @12:12:31.38 CC.seek :open @12:12:36.11 Forearm.seek :retract @12:12:37.10 Elbow.reconfigure :tight @12:12:37.14 Elbow.seek 6 @12:12:38.91 Elbow.reconfigure :limp @12:12:38.96 Forearm.seek 6 @12:12:40.00 Elbow.hold @12:12:40.06 Elbow.reconfigure :tight @12:12:40.11 Hand.open @12:12:40.86 Forearm.force -20 @12:12:40.91 Forearm.seek 6 + 311 counts @12:12:41.21 Forearm.reconfigure :tight @12:12:41.26 Forearm.seek 6 + 450 counts @12:12:41.50 SE.seek :down @12:12:43.07
Forearm.reconfigure :loose @12:12:43.12 Forearm.seek :retract @12:12:43.23 SE.stop @12:12:44.06
Tranferring DA @12:12:44.08 Beginning samplePassC @12:12:44.09 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @12:12:44.28 Elbow.seek FlushPuck::Garage @12:12:46.19 Elbow.reconfigure :loose @12:12:46.29 Forearm.seek :garage @12:12:47.28 Hand.close @12:12:47.99 Forearm.seek :retract @12:12:48.97 Elbow.reconfigure :tight @12:12:49.02 Elbow.seek CC @12:12:52.01 Elbow.reconfigure :loose @12:12:52.11 Forearm.reconfigure :tight @12:12:52.16 Forearm.seek CC @12:12:53.45 CC.seek :closed @12:12:57.97 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.38 @12:12:58.04 Hand.open @12:12:58.69 Forearm.reconfigure :loose @12:12:58.73 Forearm.seek :retract @12:12:59.73 PSV.dial PSR @12:13:01.12 Pulling 1.5ml of PSR.flush @12:13:01.13 PSR.select :flush @12:13:01.26 PS.seek 1.5ml @12:13:17.45 delay 5 seconds @12:13:22.47 PSV.dial :waste1 @12:13:23.50 PS.seek 0ml @12:13:39.69 PSV.dial PSR @12:13:40.16 PSR.select :air @12:13:40.28 PS.seek 2.5ml @12:14:06.70 PRV.dial CBV,avoiding: :ab2 @12:14:06.72
CBV.dial PRV @12:14:08.46 PSV.dial :mixloop @12:14:09.49 CTV.dial :air,avoiding: :intake @12:14:09.72 PTV.dial :PRVmixing @12:14:11.05 PS.seek 0ml @12:14:37.38 PSV.dial :air @12:14:37.86 PS.seek 2.5ml @12:15:04.25 PSV.dial :mixloop @12:15:04.73 PS.seek 0ml @12:15:31.00 PSV.dial :air @12:15:31.48 PS.seek 2.5ml @12:15:57.75 PSV.dial :mixloop @12:15:58.23 PS.seek 0ml @12:16:24.60 Garaging FlushPuck in Collection Clamp @12:16:24.67 Elbow.reconfigure :tight @12:16:24.72 Elbow.seek CC @12:16:25.01 Elbow.reconfigure :loose @12:16:25.11 Forearm.seek CC @12:16:26.17 Hand.close @12:16:26.75 CC.seek :open @12:16:31.37 Forearm.seek :retract @12:16:32.37 Elbow.reconfigure :tight @12:16:32.42 Elbow.seek :garage @12:16:35.40 Elbow.reconfigure :loose @12:16:35.50 Forearm.reconfigure :tight @12:16:35.55 Forearm.seek :garage @12:16:36.61 Hand.open @12:16:37.30 Forearm.reconfigure :loose @12:16:37.35 Forearm.seek :retract @12:16:38.40 PSV.dial :mixloop @12:16:38.47 PTV.dial :PRVmixing @12:16:38.55 PRV.dial CBV @12:16:38.58
CBV.dial PRV @12:16:38.66 CTV.dial :air,avoiding: :intake @12:16:38.68 Loading lyfil puck from tube 7 into Collection Clamp @12:16:38.92 SC.reconfigure :tight @12:16:38.96 SC.seek 7 @12:16:42.51 SC.configure :limp @12:16:42.72 Elbow.reconfigure :tight @12:16:42.77 Elbow.seek 7 @12:16:44.72 Forearm.reconfigure :tight @12:16:44.77 Forearm.seek 7 + 450 counts @12:16:45.81 SE.reconfigure :pick @12:16:45.86 SE.seek :beamBreak @12:16:48.01 SE.configure :default @12:16:48.17 Elbow.seek 7 @12:16:48.35 Forearm.seek 7 + 300 counts @12:16:48.60 Forearm.reconfigure :loose @12:16:48.65 Hand.close @12:16:49.26 SE.seek :down @12:16:49.46
Forearm.seek :retract @12:16:50.40 Elbow.seek CC @12:16:51.13 SE.stop @12:16:52.02
Elbow.reconfigure :loose @12:16:52.12 Forearm.reconfigure :tight @12:16:52.17 Forearm.seek CC @12:16:53.89 CC.seek :closed @12:16:58.41 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.17 @12:16:58.43 Hand.open @12:16:59.06 Forearm.reconfigure :loose @12:16:59.10 Forearm.seek :retract @12:17:00.08 CTV.dial CSV,avoiding: :intake @12:17:01.41 CSV.dial CTV @12:17:01.85 PS.seek 2.5ml @12:17:28.31 CS.seek 4.5ml @12:17:43.31 delay 8 seconds @12:17:51.33 PSV.dial :air @12:17:51.80 delay 2 seconds @12:17:53.82 PSV.dial :waste2 @12:17:54.56 PS.seek 0ml @12:18:20.94 CBV.dial between PRV and waste1 @12:18:21.26 CSV.dial :air @12:18:21.71 CS.seek 10ml @12:18:36.59 CSV.dial CTV @12:18:37.05 CS.seek 0ml @12:19:03.71 CBV.dial :waste1 @12:19:04.03 delay 5 seconds @12:19:09.05 CTV.dial :air,avoiding: :intake @12:19:10.45 Storing lyfil puck in Collection Clamp to tube 6 @12:19:10.63 SC.reconfigure :tight @12:19:10.67 SC.seek 6 @12:19:14.20 SC.configure :limp @12:19:14.41 Elbow.reconfigure :tight @12:19:14.46 Elbow.seek 6 @12:19:16.24 Forearm.reconfigure :tight @12:19:16.73 Forearm.seek 6 + 450 counts @12:19:17.77 SE.reconfigure :pick @12:19:17.83 SE.seek :beamBreak @12:19:19.96 SE.configure :default @12:19:20.12 Elbow.reconfigure :limp @12:19:20.18 Forearm.reconfigure :loose @12:19:20.22 Forearm.seek 6 @12:19:20.49 Elbow.hold @12:19:20.56 Elbow.reconfigure :tight @12:19:20.66 SE.seek 15.11 Pucks below Top Plate @12:19:21.12 Forearm.force -20 @12:19:21.16 Forearm.seek 6 + 306 counts @12:19:21.44 Forearm.reconfigure :tight @12:19:21.49 Forearm.seek 6 + 450 counts @12:19:21.69 SE.seek 16.11 Pucks below Top Plate @12:19:22.30 Forearm.reconfigure :loose @12:19:22.35 Forearm.seek :retract @12:19:23.28 Elbow.seek CC @12:19:24.98 Elbow.reconfigure :loose @12:19:25.08 Forearm.seek CC @12:19:26.02 Hand.close @12:19:26.62 CC.seek :open @12:19:31.39 Forearm.seek :retract @12:19:32.39 Elbow.reconfigure :tight @12:19:32.43 Elbow.seek 6 @12:19:34.19 Elbow.reconfigure :limp @12:19:34.24 Forearm.seek 6 @12:19:35.67 Elbow.hold @12:19:35.73 Elbow.reconfigure :tight @12:19:35.78 Hand.open @12:19:36.50 Forearm.force -20 @12:19:36.54 Forearm.seek 6 + 308 counts @12:19:36.83 Forearm.reconfigure :tight @12:19:36.88 Forearm.seek 6 + 450 counts @12:19:37.14 SE.seek :down @12:19:38.27
Forearm.reconfigure :loose @12:19:38.32 Forearm.seek :retract @12:19:38.75 SE.stop @12:19:39.26
PSV.dial :air @12:19:40.02 PS.seek 2.5ml @12:20:06.44 delay 1 second @12:20:07.45 PSV.dial :mixloop @12:20:07.91 delay 1 second @12:20:08.92 PSV.dial :air @12:20:09.39 delay 1 second @12:20:10.41 PSV.dial :mixloop @12:20:10.88 delay 1 second @12:20:11.91 PSV.dial :air @12:20:12.38 delay 1 second @12:20:13.40 PS.seek 0ml @12:20:39.81 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @12:20:40.00 Elbow.seek FlushPuck::Garage @12:20:41.83 Elbow.reconfigure :loose @12:20:41.93 Forearm.seek :garage @12:20:42.96 Hand.close @12:20:43.74 Forearm.seek :retract @12:20:44.75 Elbow.reconfigure :tight @12:20:44.79 Elbow.seek PC @12:20:46.08 Elbow.reconfigure :loose @12:20:46.60 Forearm.reconfigure :tight @12:20:46.67 Forearm.seek PC @12:20:47.88 PC.seek :closed @12:20:52.50 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.63 @12:20:52.56 Hand.open @12:20:53.18 Forearm.reconfigure :loose @12:20:53.23 Forearm.seek :retract @12:20:54.18 PRV.dial :air @12:20:54.64 PSV.dial :mixloop @12:20:55.18 PS.seek 0.3ml @12:20:59.16 PSV.dial PSR @12:21:00.23 Pulling 0.4ml of PSR.air @12:21:00.24 PSR.select :air @12:21:00.26 PS.seek 0.7ml @12:21:05.26 delay 3 seconds @12:21:08.27 PSR.select :air @12:21:08.30 PS.seek 2.5ml @12:21:27.53 delay 3 seconds @12:21:30.55 PSV.dial :mixloop @12:21:31.61 PTV.dial :mixing @12:21:32.37 PBV.dial :waste2 @12:21:33.35 PS.seek 0ml @12:21:59.69 Garaging FlushPuck in Processing Clamp @12:21:59.76 Elbow.reconfigure :tight @12:21:59.81 Elbow.seek PC @12:21:59.93 Elbow.reconfigure :loose @12:22:00.03 Forearm.seek PC @12:22:01.01 Hand.close @12:22:01.62 PC.seek :open @12:22:06.33 Forearm.seek :retract @12:22:07.29 Elbow.reconfigure :tight @12:22:07.79 Elbow.seek :garage @12:22:09.16 Elbow.reconfigure :loose @12:22:09.26 Forearm.reconfigure :tight @12:22:09.31 Forearm.seek :garage @12:22:10.35 Hand.open @12:22:11.05 Forearm.reconfigure :loose @12:22:11.10 Forearm.seek :retract @12:22:12.09 Loading da puck from tube 7 into Processing Clamp @12:22:12.33 SC.reconfigure :tight @12:22:12.38 SC.seek 7 @12:22:16.45 SC.configure :limp @12:22:16.67 Elbow.reconfigure :tight @12:22:16.72 Elbow.seek 7 @12:22:18.61 Forearm.reconfigure :tight @12:22:18.66 Forearm.seek 7 + 450 counts @12:22:19.69 SE.reconfigure :pick @12:22:19.75 SE.seek :beamBreak @12:22:21.99 SE.configure :default @12:22:22.15 Elbow.seek 7 @12:22:22.34 Forearm.seek 7 + 300 counts @12:22:22.58 Forearm.reconfigure :loose @12:22:22.63 Hand.close @12:22:23.26 SE.seek :down @12:22:23.45
Forearm.seek :retract @12:22:24.42 Elbow.seek PC @12:22:25.16 SE.stop @12:22:27.00
Elbow.reconfigure :loose @12:22:27.10 Forearm.reconfigure :tight @12:22:27.15 Forearm.seek PC @12:22:28.86 PC.seek :closed @12:22:33.53 Processing Clamp onSpring/offSpring for 16/26 samples, on/off torque 1.63 @12:22:33.54 Hand.open @12:22:34.18 Forearm.reconfigure :loose @12:22:34.22 Forearm.seek :retract @12:22:35.19 Heating Puck.from 7, :da in Processing Clamp to 28C for 2:00:00 @12:22:35.20 PH.seek 28C,5:00 @12:23:18.60 PH.hold 2:00:00,28C @12:23:18.65 delay 1:30 @12:24:48.69 PSR.select :air @12:24:48.71 PSV.dial PSR @12:24:49.76 Pulling 0.5ml of PSR.wash @12:24:49.77 PSR.select :wash @12:24:49.89 PS.seek 0.5ml @12:24:55.93 delay 5 seconds @12:25:00.95 PSR.select :air @12:25:01.07 PS.seek 2ml @12:25:17.18 PTV.dial :puck @12:25:17.93 PSV.dial PTV @12:25:18.70 PBV.dial :waste1 @12:25:20.09 PS.seek 1.5ml @12:25:26.03 delay 3 seconds @12:25:29.04 PBV.dial between PSV and waste1 @12:25:29.41 PS.seek 0ml @12:25:45.71 delay 5:00 @12:30:45.73 recoverPSRreagent08 @12:30:45.74 PTV.dial :puck @12:30:45.82 PBV.dial :waste1 @12:30:46.19 delay 30 seconds @12:31:16.22 PBV.dial between PSV and waste1 @12:31:16.60 PSR.select :air @12:31:16.61 PSV.dial PSR @12:31:17.39 PS.reconfigure :fast @12:31:17.43 PS.seek 2.5ml @12:31:41.17 PSV.dial PTV @12:31:41.94 delay 5 seconds @12:31:46.96 PSV.dial PSR @12:31:47.72 delay 5 seconds @12:31:52.74 PSV.dial PTV @12:31:53.52 delay 5 seconds @12:31:58.54 PSV.dial :air @12:31:59.42 PS.seek 0ml @12:32:22.65 PTV.dial :PRVmixing,via: PRV @12:32:23.97 PSV.dial :mixloop @12:32:24.42 PRV.dial :ab1 @12:32:25.18 PS.reconfigure :slow @12:32:25.23 PS.seek 0.15ml @12:32:28.86 delay 2 seconds @12:32:30.88 CBV.dial PRV @12:32:30.90
PRV.dial CBV @12:32:31.48 PS.seek 0.35ml @12:32:36.24 delay 2 seconds @12:32:38.25 CBV.dial between PRV and PSV @12:32:38.56 PRV.dial :air @12:32:39.09 PS.seek 0.9ml @12:32:50.80 PRV.dial :waste2 @12:32:51.54 PS.seek 0.5ml @12:33:00.17 addPMLReagent08 @12:33:00.18 PSV.dial :air @12:33:00.65 PS.reconfigure :default @12:33:00.70 PS.seek 2ml @12:33:16.86 PSV.dial :PML @12:33:17.33 PTV.dial :mixing @12:33:18.08 PBV.dial :waste1 @12:33:18.51 PS.seek 1.85ml @12:33:20.83 PBV.dial between PSV and waste1 @12:33:21.21 PS.seek 0ml @12:33:40.95 delay 2 seconds @12:33:42.97 Beginning samplePassC @12:33:42.99 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @12:33:43.17 Elbow.reconfigure :tight @12:33:43.21 Elbow.seek FlushPuck::Garage @12:33:44.51 Elbow.reconfigure :loose @12:33:44.61 Forearm.seek :garage @12:33:45.56 Hand.close @12:33:46.36 Forearm.seek :retract @12:33:47.35 Elbow.reconfigure :tight @12:33:47.39 Elbow.seek CC @12:33:50.27 Elbow.reconfigure :loose @12:33:50.37 Forearm.reconfigure :tight @12:33:50.42 Forearm.seek CC @12:33:51.69 CC.seek :closed @12:33:56.48 Collection Clamp onSpring/offSpring for 12/26 samples, on/off torque 2.34 @12:33:56.55 Hand.open @12:33:57.17 Forearm.reconfigure :loose @12:33:57.22 Forearm.seek :retract @12:33:58.23 PSV.dial PSR @12:33:59.42 Pulling 1.5ml of PSR.flush @12:33:59.43 PSR.select :flush @12:33:59.56 PS.seek 1.5ml @12:34:15.77 delay 5 seconds @12:34:20.79 PSV.dial :waste1 @12:34:21.27 PS.seek 0ml @12:34:37.58 PSV.dial PSR @12:34:38.05 PSR.select :air @12:34:38.17 PS.seek 2.5ml @12:35:04.62 PRV.dial CBV,avoiding: :ab2 @12:35:04.64
CBV.dial PRV @12:35:06.28 PSV.dial :mixloop @12:35:07.33 CTV.dial :air,avoiding: :intake @12:35:07.40 PTV.dial :PRVmixing @12:35:08.19 PS.seek 0ml @12:35:34.58 PSV.dial :air @12:35:35.05 PS.seek 2.5ml @12:36:01.39 PSV.dial :mixloop @12:36:01.88 PS.seek 0ml @12:36:28.26 PSV.dial :air @12:36:28.74 PS.seek 2.5ml @12:36:55.14 PSV.dial :mixloop @12:36:55.61 PS.seek 0ml @12:37:22.01 Garaging FlushPuck in Collection Clamp @12:37:22.08 Elbow.reconfigure :tight @12:37:22.13 Elbow.seek CC @12:37:22.41 Elbow.reconfigure :loose @12:37:22.51 Forearm.seek CC @12:37:23.54 Hand.close @12:37:24.11 CC.seek :open @12:37:28.72 Forearm.seek :retract @12:37:29.75 Elbow.reconfigure :tight @12:37:30.21 Elbow.seek :garage @12:37:33.24 Elbow.reconfigure :loose @12:37:33.34 Forearm.reconfigure :tight @12:37:33.39 Forearm.seek :garage @12:37:34.45 Hand.open @12:37:35.17 Forearm.reconfigure :loose @12:37:35.21 Forearm.seek :retract @12:37:36.20 delayUntil "12:41:42.96" @12:37:36.23 Whole Cell Archive sampling 1000ml @12:37:36.25 Loading wcr puck from tube 7 into Collection Clamp @12:37:36.72 Elbow.reconfigure :tight @12:37:36.77 Elbow.seek 7 @12:37:38.80 Forearm.reconfigure :tight @12:37:38.85 Forearm.seek 7 + 450 counts @12:37:39.94 SE.reconfigure :pick @12:37:40.00 SE.seek :beamBreak @12:37:42.39 SE.configure :default @12:37:42.55 Elbow.seek 7 @12:37:42.96 Forearm.seek 7 + 300 counts @12:37:43.17 Forearm.reconfigure :loose @12:37:43.22 Hand.close @12:37:43.87 SE.seek :down @12:37:44.06 Forearm.seek :retract @12:37:45.02 Elbow.seek CC @12:37:45.83 SE.stop @12:37:46.68 Elbow.reconfigure :loose @12:37:47.23 Forearm.reconfigure :tight @12:37:47.28 Forearm.seek CC @12:37:48.51 CC.seek :closed @12:37:53.06 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.18 @12:37:53.07 Hand.open @12:37:53.79 Forearm.reconfigure :loose @12:37:53.84 Forearm.seek :retract @12:37:54.84 collectSample 1000ml w/bubble point of 35psi @12:37:54.86 Priming 7ml @12:37:54.87 Intake.select :close @12:37:54.88 Exhaust.select :close @12:37:54.89 SS.hold @12:37:55.11 Calibrating pressure sensors @12:37:55.12 CTV.dial :air @12:37:55.20 SSV.dial :spit,avoiding: :exhaust @12:37:55.29 SS.reconfigure :fast @12:37:55.34 SS.seek 5ml @12:37:58.23 SSV.dial CBV @12:37:58.27 CBV.dial SSV @12:37:59.60 delay 3.8 seconds @12:38:03.53 Sensor limits: Intake max 99.6psia; Outlet max 91.5psia @12:38:03.54 SSV.dial :spit @12:38:04.89 SS.seek 0ml @12:38:07.60 SSV.dial CBV @12:38:08.93 CTV.dial :intake @12:38:09.70 SSV.dial CBV @12:38:09.73 CBV.dial SSV @12:38:09.81 Allowing 40s for 7.0ml of remaining 7.0ml @12:38:09.82 Intake.select :open @12:38:11.99 Intake @14.7psia, vacuum from 9.2 to 12.2psi @12:38:12.01 SS.reconfigure :inhaling 2.20ml/s @12:38:12.05 SS.seek 7ml @12:38:19.25 Stroke completed -- waiting for pressure to equalize ... @12:38:19.26 SS.reconfigure :exhale @12:38:19.31 SS.hold @12:38:41.82 Intake.select :close @12:38:41.98 SSV.dial :exhaust @12:38:42.74 Exhaust.select :open @12:38:42.86 SS.seek 0ml @12:38:47.44 Exhaust.select :close @12:38:47.56 Sampling 1000.0ml @12:38:47.57 Intake.select :close @12:38:47.58 Exhaust.select :close @12:38:47.59 SS.reconfigure :default @12:38:47.64 SS.hold @12:38:47.86 CTV.dial :intake @12:38:48.05 SSV.dial CBV @12:38:48.07 CBV.dial SSV @12:38:48.86 Allowing 130s for 25.0ml of remaining 1000.0ml @12:38:48.88 Intake.select :open @12:38:51.04 Intake @15.5psia, vacuum from 10.0 to 13.0psi @12:38:51.06 SS.reconfigure :inhaling 2.20ml/s @12:38:51.11 SS.seek 25ml @12:38:58.71 Vacuum too high -- waiting up to 40 seconds @12:38:58.73 SS.reconfigure :exhale @12:38:58.79 SS.hold @12:39:07.74 Intake @17.8psia, vacuum from 10.0 to 13.0psi @12:39:07.76 SS.reconfigure :inhaling 1.76ml/s @12:39:07.81 SS.seek 25ml @12:39:11.40 Vacuum too high -- waiting up to 40 seconds @12:39:11.42 SS.reconfigure :exhale @12:39:11.47 SS.hold @12:39:14.97 Intake @17.6psia, vacuum from 10.0 to 13.0psi @12:39:14.99 SS.reconfigure :inhaling 1.41ml/s @12:39:15.04 SS.seek 25ml @12:39:18.59 Vacuum too high -- waiting up to 40 seconds @12:39:18.60 SS.reconfigure :exhale @12:39:18.66 SS.hold @12:39:21.79 Intake @17.1psia, vacuum from 10.0 to 13.0psi @12:39:21.81 SS.reconfigure :inhaling 1.13ml/s @12:39:21.86 SS.seek 25ml @12:39:28.62 Vacuum too high -- waiting up to 40 seconds @12:39:28.64 SS.reconfigure :exhale @12:39:28.69 SS.hold @12:39:33.82 Intake @18.8psia, vacuum from 10.0 to 13.0psi @12:39:33.84 SS.reconfigure :inhaling 0.91ml/s @12:39:33.89 SS.seek 25ml @12:39:34.36 Stroke completed -- waiting for pressure to equalize ... @12:39:34.37 SS.reconfigure :exhale @12:39:34.42 SS.hold @12:39:57.34 Intake.select :close @12:39:57.50 SSV.dial :exhaust @12:39:58.28 Exhaust.select :open @12:39:58.40 SS.seek 0ml @12:40:07.20 Exhaust.select :close @12:40:07.31 SSV.dial CBV @12:40:07.33 CBV.dial SSV @12:40:08.11 Allowing 130s for 25.0ml of remaining 975.0ml @12:40:08.13 Intake.select :open @12:40:10.29 Intake @16.0psia, vacuum from 10.0 to 13.0psi @12:40:10.31 SS.reconfigure :inhaling 0.91ml/s @12:40:10.36 SS.seek 25ml @12:40:40.41 Stroke completed -- waiting for pressure to equalize ... @12:40:40.42 SS.reconfigure :exhale @12:40:40.47 SS.hold @12:41:17.31 Intake.select :close @12:41:17.47 SSV.dial :exhaust @12:41:18.23 Exhaust.select :open @12:41:18.35 SS.seek 0ml @12:41:27.19 Exhaust.select :close @12:41:27.30 SSV.dial CBV @12:41:27.32 CBV.dial SSV @12:41:28.09 Allowing 130s for 25.0ml of remaining 950.0ml @12:41:28.11 Intake.select :open @12:41:30.28 Intake @16.2psia, vacuum from 10.0 to 13.0psi @12:41:30.30 SS.reconfigure :inhaling 0.91ml/s @12:41:30.35 SS.seek 25ml @12:41:42.98 recoverPRVreagent08 @12:41:42.99 PTV.dial :mixing @12:41:43.76 PBV.dial :waste1 @12:41:44.15 delay 30 seconds @12:42:00.39 Stroke completed -- waiting for pressure to equalize ... @12:42:00.40 SS.reconfigure :exhale @12:42:00.45 SS.hold @12:42:14.17 PBV.dial between PSV and waste1 @12:42:14.54 PSV.dial :air @12:42:15.01 PS.reconfigure :fast @12:42:15.06 PS.seek 2.5ml @12:42:37.68 Intake.select :close @12:42:37.84 SSV.dial :exhaust @12:42:38.62 Exhaust.select :open @12:42:38.74 SS.seek 0ml @12:42:39.04 PSV.dial :mixloop @12:42:39.51 delay 2 seconds @12:42:41.53 PSV.dial :air @12:42:41.99 delay 2 seconds @12:42:44.01 PSV.dial :mixloop @12:42:44.48 delay 2 seconds @12:42:46.49 PSV.dial :air @12:42:46.97 PS.seek 0ml @12:42:47.44 Exhaust.select :close @12:42:47.55 SSV.dial CBV @12:42:47.57 CBV.dial SSV @12:42:48.34 Allowing 130s for 25.0ml of remaining 925.0ml @12:42:48.36 Intake.select :open @12:42:50.53 Intake @16.1psia, vacuum from 10.0 to 13.0psi @12:42:50.55 SS.reconfigure :inhaling 0.91ml/s @12:42:50.59 SS.seek 25ml @12:43:10.66 washCycleDA #1 of 2 @12:43:10.67 PSR.select :air @12:43:10.69 PSV.dial PSR @12:43:12.04 Pulling 0.5ml of PSR.wash @12:43:12.05 PSR.select :wash @12:43:12.16 PS.reconfigure :default @12:43:12.21 PS.seek 0.5ml @12:43:12.52 Vacuum too high -- waiting up to 40 seconds @12:43:12.54 SS.reconfigure :exhale @12:43:12.59 SS.hold @12:43:18.13 delay 5 seconds @12:43:21.33 Intake @18.1psia, vacuum from 10.0 to 13.0psi @12:43:21.35 SS.reconfigure :inhaling 0.73ml/s @12:43:21.40 SS.seek 25ml @12:43:23.15 PSR.select :air @12:43:23.26 PS.seek 2ml @12:43:30.27 Vacuum too high -- waiting up to 40 seconds @12:43:30.29 SS.reconfigure :exhale @12:43:30.34 SS.hold @12:43:33.98 Intake @18.0psia, vacuum from 10.0 to 13.0psi @12:43:34.00 SS.reconfigure :inhaling 0.59ml/s @12:43:34.05 SS.seek 25ml @12:43:35.86 Stroke completed -- waiting for pressure to equalize ... @12:43:35.87 SS.reconfigure :exhale @12:43:35.92 SS.hold @12:43:39.38 PTV.dial :puck @12:43:40.15 PSV.dial PTV @12:43:40.95 PBV.dial :waste1 @12:43:41.42 PS.seek 1.5ml @12:43:47.35 delay 3 seconds @12:43:50.37 PBV.dial between PSV and waste1 @12:43:50.75 PS.seek 0ml @12:43:58.60 Intake.select :close @12:43:58.75 SSV.dial :exhaust @12:43:59.51 Exhaust.select :open @12:43:59.63 SS.seek 0ml @12:44:06.88 delay 2:00 @12:44:08.45 Exhaust.select :close @12:44:08.56 SSV.dial CBV @12:44:08.60 CBV.dial SSV @12:44:09.36 Allowing 130s for 25.0ml of remaining 900.0ml @12:44:09.37 Intake.select :open @12:44:11.95 Intake @16.1psia, vacuum from 10.0 to 13.0psi @12:44:11.97 SS.reconfigure :inhaling 0.59ml/s @12:44:12.02 SS.seek 25ml @12:44:56.89 Stroke completed -- waiting for pressure to equalize ... @12:44:56.90 SS.reconfigure :exhale @12:44:56.95 SS.hold @12:45:24.08 Intake.select :close @12:45:24.24 SSV.dial :exhaust @12:45:25.03 Exhaust.select :open @12:45:25.15 SS.seek 0ml @12:45:34.17 Exhaust.select :close @12:45:34.28 SSV.dial CBV @12:45:34.30 CBV.dial SSV @12:45:35.08 Allowing 130s for 25.0ml of remaining 875.0ml @12:45:35.09 Intake.select :open @12:45:37.26 Intake @16.1psia, vacuum from 10.0 to 13.0psi @12:45:37.28 SS.reconfigure :inhaling 0.59ml/s @12:45:37.33 SS.seek 25ml @12:46:06.91 recoverPSRreagent08 @12:46:06.92 PTV.dial :puck @12:46:06.99 PBV.dial :waste1 @12:46:07.37 delay 30 seconds @12:46:21.82 Stroke completed -- waiting for pressure to equalize ... @12:46:21.84 SS.reconfigure :exhale @12:46:21.88 SS.hold @12:46:37.38 PBV.dial between PSV and waste1 @12:46:37.75 PSR.select :air @12:46:37.77 PSV.dial PSR @12:46:38.54 PS.reconfigure :fast @12:46:38.59 PS.seek 2.5ml @12:46:46.47 Intake.select :close @12:46:46.63 SSV.dial :exhaust @12:46:47.40 Exhaust.select :open @12:46:47.52 SS.seek 0ml @12:46:56.56 Exhaust.select :close @12:46:56.67 SSV.dial CBV @12:46:56.70 CBV.dial SSV @12:46:57.46 Allowing 130s for 25.0ml of remaining 850.0ml @12:46:57.48 Intake.select :open @12:46:59.65 Intake @16.2psia, vacuum from 10.0 to 13.0psi @12:46:59.67 SS.reconfigure :inhaling 0.59ml/s @12:46:59.72 SS.seek 25ml @12:47:02.47 PSV.dial PTV @12:47:03.36 delay 5 seconds @12:47:08.39 PSV.dial PSR @12:47:09.25 delay 5 seconds @12:47:14.28 PSV.dial PTV @12:47:15.02 delay 5 seconds @12:47:20.04 PSV.dial :air @12:47:20.93 PS.seek 0ml @12:47:23.80 Vacuum too high -- waiting up to 40 seconds @12:47:23.82 SS.reconfigure :exhale @12:47:23.87 SS.hold @12:47:30.08 Intake @16.6psia, vacuum from 10.0 to 13.0psi @12:47:30.10 SS.reconfigure :inhaling 0.47ml/s @12:47:30.15 SS.seek 25ml @12:47:44.29 washCycleDA #2 of 2 @12:47:44.30 PSR.select :air @12:47:44.32 PSV.dial PSR @12:47:45.66 Pulling 0.5ml of PSR.wash @12:47:45.67 PSR.select :wash @12:47:45.78 PS.reconfigure :default @12:47:45.83 PS.seek 0.5ml @12:47:51.75 delay 5 seconds @12:47:55.63 Stroke completed -- waiting for pressure to equalize ... @12:47:55.65 SS.reconfigure :exhale @12:47:55.69 SS.hold @12:47:56.77 PSR.select :air @12:47:56.95 PS.seek 2ml @12:48:13.16 PTV.dial :puck @12:48:13.24 PSV.dial PTV @12:48:14.00 PBV.dial :waste1 @12:48:14.44 PS.seek 1.5ml @12:48:18.26 Intake.select :close @12:48:18.42 SSV.dial :exhaust @12:48:19.19 Exhaust.select :open @12:48:19.31 SS.seek 0ml @12:48:20.35 delay 3 seconds @12:48:23.36 PBV.dial between PSV and waste1 @12:48:23.74 PS.seek 0ml @12:48:28.13 Exhaust.select :close @12:48:28.24 SSV.dial CBV @12:48:28.26 CBV.dial SSV @12:48:29.04 Allowing 130s for 25.0ml of remaining 825.0ml @12:48:29.06 Intake.select :open @12:48:31.22 Intake @16.0psia, vacuum from 10.0 to 13.0psi @12:48:31.24 SS.reconfigure :inhaling 0.47ml/s @12:48:31.29 SS.seek 25ml @12:48:39.88 delay 2:00 @12:49:26.85 Stroke completed -- waiting for pressure to equalize ... @12:49:26.86 SS.reconfigure :exhale @12:49:26.91 SS.hold @12:49:58.01 Intake.select :close @12:49:58.17 SSV.dial :exhaust @12:49:58.93 Exhaust.select :open @12:49:59.05 SS.seek 0ml @12:50:08.04 Exhaust.select :close @12:50:08.15 SSV.dial CBV @12:50:08.18 CBV.dial SSV @12:50:08.95 Allowing 130s for 25.0ml of remaining 800.0ml @12:50:08.96 Intake.select :open @12:50:11.59 Intake @16.0psia, vacuum from 10.0 to 13.0psi @12:50:11.61 SS.reconfigure :inhaling 0.47ml/s @12:50:11.66 SS.seek 25ml @12:50:39.90 recoverPSRreagent08 @12:50:39.91 PTV.dial :puck @12:50:39.98 PBV.dial :waste1 @12:50:40.36 delay 30 seconds @12:50:58.11 Vacuum too high -- waiting up to 40 seconds @12:50:58.13 SS.reconfigure :exhale @12:50:58.18 SS.hold @12:51:10.38 PBV.dial between PSV and waste1 @12:51:10.73 PSR.select :air @12:51:10.75 PSV.dial PSR @12:51:11.52 PS.reconfigure :fast @12:51:11.57 PS.seek 2.5ml @12:51:17.15 Intake @16.4psia, vacuum from 10.0 to 13.0psi @12:51:17.17 SS.reconfigure :inhaling 0.38ml/s @12:51:17.22 SS.seek 25ml @12:51:28.17 Stroke completed -- waiting for pressure to equalize ... @12:51:28.19 SS.reconfigure :exhale @12:51:28.24 SS.hold @12:51:35.43 PSV.dial PTV @12:51:36.20 delay 5 seconds @12:51:41.22 PSV.dial PSR @12:51:41.97 delay 5 seconds @12:51:46.98 PSV.dial PTV @12:51:47.75 delay 5 seconds @12:51:49.14 Intake.select :close @12:51:49.30 SSV.dial :exhaust @12:51:50.07 Exhaust.select :open @12:51:50.19 SS.seek 0ml @12:51:52.77 PSV.dial :air @12:51:53.55 PS.seek 0ml @12:51:59.21 Exhaust.select :close @12:51:59.32 SSV.dial CBV @12:51:59.35 CBV.dial SSV @12:52:00.12 Allowing 130s for 25.0ml of remaining 775.0ml @12:52:00.13 Intake.select :open @12:52:02.30 Intake @15.9psia, vacuum from 10.0 to 13.0psi @12:52:02.32 SS.reconfigure :inhaling 0.38ml/s @12:52:02.37 SS.seek 25ml @12:52:17.00 getPMLReagent08 @12:52:17.01 PRV.dial :air @12:52:17.48 delay 5 seconds @12:52:22.50 PTV.dial :PRVmixing @12:52:23.84 delay 5 seconds @12:52:28.86 PRV.dial :ab2 @12:52:29.33 PTV.dial :PRVmixing @12:52:29.40 PSV.dial :mixloop @12:52:29.89 PS.reconfigure :default @12:52:29.94 PS.seek 0.3ml @12:52:33.91 delay 2 seconds @12:52:35.93 PRV.dial :air @12:52:36.45 PS.seek 0.55ml @12:52:39.82 addPMLReagent08 @12:52:39.83 PSV.dial :air @12:52:40.30 PS.seek 2ml @12:52:55.87 PSV.dial :PML @12:52:56.36 PTV.dial :mixing @12:52:57.12 PBV.dial :waste1 @12:52:57.56 PS.seek 1.85ml @12:52:59.87 PBV.dial between PSV and waste1 @12:53:00.25 PS.seek 0ml @12:53:10.17 Stroke completed -- waiting for pressure to equalize ... @12:53:10.19 SS.reconfigure :exhale @12:53:10.23 SS.hold @12:53:20.04 delay 2 seconds @12:53:22.06 delay 8:00 @12:53:50.43 Intake.select :close @12:53:50.98 SSV.dial :exhaust @12:53:51.82 Exhaust.select :open @12:53:51.94 SS.seek 0ml @12:54:01.02 Exhaust.select :close @12:54:01.13 SSV.dial CBV @12:54:01.15 CBV.dial SSV @12:54:01.93 Allowing 130s for 25.0ml of remaining 750.0ml @12:54:01.94 Intake.select :open @12:54:04.11 Intake @15.9psia, vacuum from 10.0 to 13.0psi @12:54:04.13 SS.reconfigure :inhaling 0.38ml/s @12:54:04.18 SS.seek 25ml @12:54:47.51 Vacuum too high -- waiting up to 40 seconds @12:54:47.52 SS.reconfigure :exhale @12:54:47.58 SS.hold @12:55:04.51 Intake @16.1psia, vacuum from 10.0 to 13.0psi @12:55:04.53 SS.reconfigure :inhaling 0.31ml/s @12:55:04.58 SS.seek 25ml @12:55:35.97 Stroke completed -- waiting for pressure to equalize ... @12:55:35.98 SS.reconfigure :exhale @12:55:36.03 SS.hold @12:56:10.85 Intake.select :close @12:56:11.01 SSV.dial :exhaust @12:56:11.80 Exhaust.select :open @12:56:11.92 SS.seek 0ml @12:56:20.73 Exhaust.select :close @12:56:20.84 SSV.dial CBV @12:56:20.86 CBV.dial SSV @12:56:21.64 Allowing 130s for 25.0ml of remaining 725.0ml @12:56:21.66 Intake.select :open @12:56:23.82 Intake @15.8psia, vacuum from 10.0 to 13.0psi @12:56:23.84 SS.reconfigure :inhaling 0.31ml/s @12:56:23.89 SS.seek 25ml @12:57:48.20 Vacuum too high -- waiting up to 40 seconds @12:57:48.21 SS.reconfigure :exhale @12:57:48.27 SS.hold @12:58:16.89 Intake @16.7psia, vacuum from 10.0 to 13.0psi @12:58:16.91 SS.reconfigure :inhaling 0.25ml/s @12:58:16.96 SS.seek 25ml @12:58:21.89 Stroke completed -- waiting for pressure to equalize ... @12:58:21.90 SS.reconfigure :exhale @12:58:21.95 SS.hold @12:58:31.73 SS.reconfigure :default @12:58:31.78 SS.hold @12:58:31.85 Intake.select :close @12:58:32.01 ShallowSampler::Clogged in WCR -- Filtering too Slowly after 300.0ml @12:58:32.02 SSV.dial :exhaust @12:58:32.79 Exhaust.select :open @12:58:32.91 SS.reconfigure :exhale @12:58:32.96 SS.seek 0ml @12:58:42.07 Exhaust.select :close @12:58:42.18 Sampled 300.0ml @12:58:42.20 Drying puck before fixing for 5:00 @12:58:42.21 CBV.dial :waste2b @12:58:42.98 delay 1:00 @12:59:43.01 CSV.dial :air @12:59:43.47 CS.reconfigure :fast @12:59:43.52 CS.seek 10ml @13:00:08.25 Pumping wcr puck from tube 7 up to 35psi... @13:00:08.26 CBV.dial SSV @13:00:09.53 CBV.dial between CSV and waste2b @13:00:10.50 CSV.dial between air and CTV @13:00:10.83 CS.seek 7.192ml @13:00:18.12 CTV.dial CSV,avoiding: :air @13:00:18.15 CSV.dial CTV @13:00:19.00 CS.reconfigure :pressurize @13:00:19.05 CS.seek 0ml @13:00:37.90 CSV.dial :air @13:00:38.34 CS.reconfigure :fast @13:00:38.39 CS.seek 10ml @13:01:02.80 CSV.dial between air and CTV @13:01:03.12 CS.seek 5.282ml @13:01:14.69 CTV.dial CSV,avoiding: :air @13:01:14.71 CSV.dial CTV @13:01:15.64 CS.reconfigure :pressurize @13:01:15.69 CS.seek 0ml @13:01:22.08 recoverPRVreagent08 @13:01:22.09 PTV.dial :mixing @13:01:22.18 PBV.dial :waste1 @13:01:22.55 delay 30 seconds @13:01:29.80 CSV.dial :air @13:01:30.25 CS.reconfigure :fast @13:01:30.30 CS.seek 10ml @13:01:52.58 PBV.dial between PSV and waste1 @13:01:52.96 PSV.dial :air @13:01:53.43 PS.reconfigure :fast @13:01:53.48 PS.seek 2.5ml @13:01:54.77 CSV.dial between air and CTV @13:01:55.09 CS.seek 5.446ml @13:02:06.31 CTV.dial CSV,avoiding: :air @13:02:06.33 CSV.dial CTV @13:02:06.68 CS.reconfigure :pressurize @13:02:06.74 CS.seek 0ml @13:02:17.42 PSV.dial :mixloop @13:02:17.89 delay 2 seconds @13:02:19.91 PSV.dial :air @13:02:20.38 delay 2 seconds @13:02:21.21 CSV.dial :air @13:02:21.67 CS.reconfigure :fast @13:02:21.72 CS.seek 10ml @13:02:22.39 PSV.dial :mixloop @13:02:22.86 delay 2 seconds @13:02:24.88 PSV.dial :air @13:02:25.35 PS.seek 0ml @13:02:46.08 CSV.dial between air and CTV @13:02:46.40 CS.seek 5.437ml @13:02:48.86 washCycleDA #1 of 3 @13:02:48.87 PSR.select :air @13:02:48.89 PSV.dial PSR @13:02:50.22 Pulling 0.5ml of PSR.wash @13:02:50.23 PSR.select :wash @13:02:50.35 PS.reconfigure :default @13:02:50.39 PS.seek 0.5ml @13:02:56.39 delay 5 seconds @13:02:57.56 CTV.dial CSV,avoiding: :air @13:02:57.58 CSV.dial CTV @13:02:57.92 CS.reconfigure :pressurize @13:02:57.97 CS.seek 0ml @13:03:01.41 PSR.select :air @13:03:01.53 PS.seek 2ml @13:03:12.86 CSV.dial :air @13:03:13.32 CS.reconfigure :default @13:03:13.37 CS.hold @13:03:13.49 ShallowSampler::Error in MAIN -- Puck.from 7, :wcr reached only 18.8 of 35psi after pumping in 40ml of gas @13:03:13.51 Waiting to Resume from Checkpoint "Pumping wcr puck from tube 7 up to 35psi..." @13:03:13.52 BadNews.email "Puck.from 7, :wcr reached only 18.8 of 35psi after pumping in 40ml of gas",Subject: "ShallowSampler::Error in MAIN" @13:03:17.70 PTV.dial :puck @13:03:18.50 PSV.dial PTV @13:03:19.29 PBV.dial :waste1 @13:03:19.76 PS.seek 1.5ml @13:03:19.85 upsync @13:03:25.67 delay 3 seconds @13:03:28.69 PBV.dial between PSV and waste1 @13:03:29.07 PS.seek 0ml @13:03:45.21 delay 2:00 @13:05:45.24 recoverPSRreagent08 @13:05:45.25 PTV.dial :puck @13:05:45.33 PBV.dial :waste1 @13:05:45.70 delay 30 seconds @13:06:15.73 PBV.dial between PSV and waste1 @13:06:16.11 PSR.select :air @13:06:16.13 PSV.dial PSR @13:06:16.90 PS.reconfigure :fast @13:06:16.95 PS.seek 2.5ml @13:06:40.86 PSV.dial PTV @13:06:41.75 delay 5 seconds @13:06:46.77 PSV.dial PSR @13:06:47.53 delay 5 seconds @13:06:52.55 PSV.dial PTV @13:06:53.31 delay 5 seconds @13:06:58.33 PSV.dial :air @13:06:59.10 PS.seek 0ml @13:07:22.30 washCycleDA #2 of 3 @13:07:22.31 PSR.select :air @13:07:22.32 PSV.dial PSR @13:07:23.65 Pulling 0.5ml of PSR.wash @13:07:23.67 PSR.select :wash @13:07:23.79 PS.reconfigure :default @13:07:23.83 PS.seek 0.5ml @13:07:29.76 delay 5 seconds @13:07:34.78 PSR.select :air @13:07:34.91 PS.seek 2ml @13:07:51.01 PTV.dial :puck @13:07:51.09 PSV.dial PTV @13:07:51.96 PBV.dial :waste1 @13:07:52.91 PS.seek 1.5ml @13:07:58.89 delay 3 seconds @13:08:01.91 PBV.dial between PSV and waste1 @13:08:02.28 PS.seek 0ml @13:08:18.64 delay 2:00 @13:10:18.67 recoverPSRreagent08 @13:10:18.68 PTV.dial :puck @13:10:18.74 PBV.dial :waste1 @13:10:19.12 delay 30 seconds @13:10:49.15 PBV.dial between PSV and waste1 @13:10:49.52 PSR.select :air @13:10:49.54 PSV.dial PSR @13:10:50.30 PS.reconfigure :fast @13:10:50.35 PS.seek 2.5ml @13:11:14.41 PSV.dial PTV @13:11:15.18 delay 5 seconds @13:11:20.20 PSV.dial PSR @13:11:20.96 delay 5 seconds @13:11:25.98 PSV.dial PTV @13:11:26.74 delay 5 seconds @13:11:31.76 PSV.dial :air @13:11:32.55 PS.seek 0ml @13:11:55.79 washCycleDA #3 of 3 @13:11:55.80 PSR.select :air @13:11:55.82 PSV.dial PSR @13:11:57.15 Pulling 0.5ml of PSR.wash @13:11:57.16 PSR.select :wash @13:11:57.28 PS.reconfigure :default @13:11:57.33 PS.seek 0.5ml @13:12:03.23 delay 5 seconds @13:12:08.25 PSR.select :air @13:12:08.37 PS.seek 2ml @13:12:24.47 PTV.dial :puck @13:12:24.55 PSV.dial PTV @13:12:25.30 PBV.dial :waste1 @13:12:25.74 PS.seek 1.5ml @13:12:31.69 delay 3 seconds @13:12:34.71 PBV.dial between PSV and waste1 @13:12:35.09 PS.seek 0ml @13:12:51.26 delay 2:00 @13:14:51.29 recoverPSRreagent08 @13:14:51.30 PTV.dial :puck @13:14:51.36 PBV.dial :waste1 @13:14:51.74 delay 30 seconds @13:15:21.77 PBV.dial between PSV and waste1 @13:15:22.14 PSR.select :air @13:15:22.16 PSV.dial PSR @13:15:22.93 PS.reconfigure :fast @13:15:22.98 PS.seek 2.5ml @13:15:46.72 PSV.dial PTV @13:15:47.49 delay 5 seconds @13:15:52.51 PSV.dial PSR @13:15:53.25 delay 5 seconds @13:15:58.28 PSV.dial PTV @13:15:59.14 delay 5 seconds @13:16:04.16 PSV.dial :air @13:16:05.06 PS.seek 0ml @13:16:28.53 Gate.power :camera,:ON @13:16:28.55 Mixing DA Substrates @13:16:28.56 PSR.select :air @13:16:28.57 PSV.dial PSR @13:16:30.11 Pulling 0.25ml of PSR.sub1 @13:16:30.13 PSR.select :sub1 @13:16:30.33 PS.reconfigure :default @13:16:30.44 PS.seek 0.25ml @13:16:35.00 delay 5 seconds @13:16:40.02 Pulling 0.25ml of PSR.sub2 @13:16:40.04 PSR.select :sub2 @13:16:40.15 PS.seek 0.5ml @13:16:43.56 delay 5 seconds @13:16:48.59 PSR.select :air @13:16:48.70 PS.seek 2ml @13:17:04.81 PTV.dial :puck @13:17:04.89 PSV.dial PTV @13:17:05.66 PBV.dial :waste1 @13:17:06.11 PS.seek 1.5ml @13:17:12.10 delay 3 seconds @13:17:15.11 PBV.dial between PSV and waste1 @13:17:15.49 PS.seek 0ml @13:17:31.69 delay 10 seconds @13:17:41.72 recoverPSRreagent08 @13:17:41.73 PTV.dial :puck @13:17:41.80 PBV.dial :waste1 @13:17:42.17 delay 30 seconds @13:18:12.20 PBV.dial between PSV and waste1 @13:18:12.59 PSR.select :air @13:18:12.61 PSV.dial PSR @13:18:13.40 PS.reconfigure :fast @13:18:13.45 PS.seek 2.5ml @13:18:37.59 PSV.dial PTV @13:18:38.38 delay 5 seconds @13:18:43.40 PSV.dial PSR @13:18:44.16 delay 5 seconds @13:18:49.18 PSV.dial PTV @13:18:49.94 delay 5 seconds @13:18:54.96 PSV.dial :air @13:18:55.74 PS.seek 0ml @13:19:19.53 Additional air clear @13:19:19.54 PSR.select :air @13:19:19.56 PSV.dial PSR @13:19:20.91 Pulling 2ml of PSR.air @13:19:20.92 PSR.select :air @13:19:20.94 PS.reconfigure :default @13:19:20.99 PS.seek 2ml @13:19:42.19 delay 5 seconds @13:19:47.21 PTV.dial :puck @13:19:47.30 PSV.dial PTV @13:19:48.19 PBV.dial :waste1 @13:19:48.64 PS.seek 1.5ml @13:19:54.59 delay 3 seconds @13:19:57.61 PBV.dial between PSV and waste1 @13:19:58.00 delay 1 second @13:19:59.02 PSV.dial :waste1 @13:20:00.08 PS.seek 0ml @13:20:16.45 Processing Puck Heater at 28.1C before imaging @13:20:16.46 PH.coast @13:20:16.58 Imaging da puck in Processing Clamp while storing it to tube 6 @13:20:16.75 SC.reconfigure :tight @13:20:16.80 SC.seek 6 @13:20:20.37 SC.configure :limp @13:20:20.58 Elbow.reconfigure :tight @13:20:21.06 Elbow.seek 6 @13:20:22.90 Forearm.reconfigure :tight @13:20:22.95 Forearm.seek 6 + 450 counts @13:20:24.04 SE.reconfigure :pick @13:20:24.10 SE.seek :beamBreak @13:20:26.25 SE.configure :default @13:20:26.41 Elbow.reconfigure :limp @13:20:26.46 Forearm.reconfigure :loose @13:20:26.50 Forearm.seek 6 @13:20:26.78 Elbow.hold @13:20:26.84 Elbow.reconfigure :tight @13:20:26.94 SE.seek 16.11 Pucks below Top Plate @13:20:27.46 Forearm.force -20 @13:20:27.51 Forearm.seek 6 + 311 counts @13:20:27.80 Forearm.reconfigure :tight @13:20:27.85 Forearm.seek 6 + 450 counts @13:20:28.05 SE.seek 17.11 Pucks below Top Plate @13:20:28.66 Forearm.reconfigure :loose @13:20:28.71 Forearm.seek :retract @13:20:29.64 Elbow.seek PC @13:20:32.18 Elbow.reconfigure :loose @13:20:32.28 Forearm.seek PC @13:20:33.23 Hand.close @13:20:33.83 PC.seek :open @13:20:38.66 Forearm.seek :retract @13:20:39.64 Elbow.reconfigure :tight @13:20:39.69 Elbow.seek 6 @13:20:42.33 Forearm.reconfigure :tight @13:20:42.38 Forearm.seek 6 + 450 counts @13:20:43.34 Hand.open @13:20:44.01 SE.seek 16.76 Pucks below Top Plate @13:20:44.78 SE.reconfigure :seal @13:20:44.83 SE.seek 15.79 Pucks below Top Plate @13:20:45.39 Lights, camera, ACTION! @13:20:45.42 PuckCamera: snap -bin=2,2 -auto /var/log/esp/da23dec2113h550ml.tif @13:21:04.88 Waiting >20 seconds for da to relinquish FlushPuck @13:21:08.87 SX HX9/16 (USB:33): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 20.145 seconds /var/log/esp/da23dec2113h550ml.tif: TIFF Upload Complete @13:21:08.90 PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da23dec2113h550ml15s.tif @13:21:24.55 SX HX9/16 (USB:33): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15 seconds /var/log/esp/midres/da23dec2113h550ml15s.tif: TIFF Upload Complete @13:21:24.58 PuckCamera: snap -bin=2,2 20.0 /var/log/esp/midres/da23dec2113h550ml20s.tif @13:21:45.19 SX HX9/16 (USB:33): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 20 seconds /var/log/esp/midres/da23dec2113h550ml20s.tif: TIFF Upload Complete @13:21:45.20 Gate.power :camera,:OFF @13:21:45.23 DAfans.email "20.145 sec auto-exposure",Subject: "da23dec2113h550ml images!" @13:21:45.67 Elbow.seek 6 @13:21:45.90 Forearm.seek 6 + 450 counts @13:21:45.99 SE.reconfigure :gently @13:21:46.04 SE.seek 16.51 Pucks below Top Plate @13:21:46.73 Elbow.reconfigure :limp @13:21:46.79 Forearm.reconfigure :loose @13:21:46.84 Forearm.seek 6 @13:21:47.16 Elbow.hold @13:21:47.23 Elbow.reconfigure :tight @13:21:47.29 SE.reconfigure :default @13:21:47.36 SE.seek 16.69 Pucks below Top Plate @13:21:47.85 Forearm.force -20 @13:21:47.89 Forearm.seek 6 + 276 counts @13:21:48.18 Forearm.reconfigure :tight @13:21:48.23 Forearm.seek 6 + 450 counts @13:21:48.49 SE.seek :down @13:21:49.71 Forearm.reconfigure :loose @13:21:49.81 Forearm.seek :retract @13:21:50.17 SE.stop @13:21:50.93 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @13:21:51.33 Elbow.seek FlushPuck::Garage @13:21:53.38 Elbow.reconfigure :loose @13:21:54.74 Forearm.seek :garage @13:21:55.83 Hand.close @13:21:56.72 Forearm.seek :retract @13:21:57.80 Elbow.reconfigure :tight @13:21:58.00 Elbow.seek PC @13:21:59.57 Elbow.reconfigure :loose @13:21:59.87 Forearm.reconfigure :tight @13:21:59.98 Forearm.seek PC @13:22:01.68 PC.seek :closed @13:22:06.47 Processing Clamp onSpring/offSpring for 16/26 samples, on/off torque 1.62 @13:22:06.60 Hand.open @13:22:07.34 Forearm.reconfigure :loose @13:22:07.43 Forearm.seek :retract @13:22:08.42 PSR.select :air @13:22:08.46 PSV.dial PSR @13:22:08.98 Pulling 0.5ml of PSR.flush @13:22:09.00 PSR.select :flush @13:22:09.18 PS.seek 0.5ml @13:22:15.18 delay 5 seconds @13:22:18.54 upsync @13:22:20.21 PSR.select :air @13:22:20.33 PS.seek 2.5ml @13:22:41.62 delay 1 second @13:22:42.64 PRV.dial :waste2,avoiding: :ab2 @13:22:44.53 PTV.dial :PRVmixing @13:22:45.85 PSV.dial :mixloop @13:22:46.89 PS.seek :empty @13:23:13.27 PSR.select :air @13:23:13.29 PSV.dial PSR @13:23:14.34 Pulling 0.5ml of PSR.flush @13:23:14.35 PSR.select :flush @13:23:14.47 PS.seek 0.5ml @13:23:20.50 delay 5 seconds @13:23:25.52 PSR.select :air @13:23:25.64 PS.seek 2.5ml @13:23:46.93 delay 1 second @13:23:47.95 PBV.dial :waste1 @13:23:48.32 PTV.dial :mixing @13:23:49.54 PSV.dial :mixloop @13:23:50.61 PS.seek :empty @13:24:16.99 PSV.dial PSR @13:24:18.17 PSR.select :flush @13:24:18.29 PS.seek 1ml @13:24:29.43 PSR.select :air @13:24:29.55 PS.seek 2.5ml @13:24:45.68 PSV.dial PTV @13:24:46.43 PTV.dial :puck @13:24:47.18 PBV.dial :waste1 @13:24:47.20 PS.seek 0ml @13:25:13.59 PSV.dial PSR @13:25:14.35 PSR.select :flush @13:25:14.48 PS.seek 0.5ml @13:25:20.37 PSR.select :air @13:25:20.49 PS.seek 2.5ml @13:25:41.71 PSV.dial PTV @13:25:42.48 PBV.dial PSV @13:25:43.05 PS.seek 0ml @13:26:09.58 PSV.dial PBV @13:26:10.06 PS.seek 2.5ml @13:26:36.40 PSV.dial :waste1 @13:26:37.14 PS.seek 0ml @13:27:03.52 PSV.dial PSR @13:27:03.99 PSR.select :air @13:27:04.02 PS.seek 2.5ml @13:27:30.36 PSV.dial PTV @13:27:31.10 PBV.dial PSV @13:27:31.18 PS.seek 0ml @13:27:57.74 PSV.dial PBV @13:27:58.22 PS.seek 2.5ml @13:28:24.49 PSV.dial :waste1 @13:28:25.23 PS.seek 0ml @13:28:51.55 PSV.dial PSR @13:28:52.02 PSR.select :flush @13:28:52.14 PS.seek 1ml @13:29:03.14 PSR.select :air @13:29:03.26 PS.seek 2.5ml @13:29:19.39 PSV.dial PTV @13:29:20.13 PTV.dial :puck @13:29:20.32 PBV.dial :waste1 @13:29:20.89 PS.seek 0ml @13:29:47.21 PSV.dial PSR @13:29:48.41 PSR.select :flush @13:29:48.53 PS.seek 0.5ml @13:29:54.55 PSR.select :air @13:29:54.67 PS.seek 2.5ml @13:30:15.89 PSV.dial PTV @13:30:16.66 PBV.dial PSV @13:30:17.23 PS.seek 0ml @13:30:43.58 PSV.dial PBV @13:30:44.06 PS.seek 2.5ml @13:31:10.36 PSV.dial :waste1 @13:31:11.10 PS.seek 0ml @13:31:37.49 PSV.dial PSR @13:31:37.95 PSR.select :air @13:31:37.98 PS.seek 2.5ml @13:32:04.33 PSV.dial PTV @13:32:05.06 PBV.dial PSV @13:32:05.26 PS.seek 0ml @13:32:31.64 PSV.dial PBV @13:32:32.12 PS.seek 2.5ml @13:32:58.42 PSV.dial :waste1 @13:32:59.16 PS.seek 0ml @13:33:25.55 Garaging FlushPuck in Processing Clamp @13:33:25.62 Elbow.reconfigure :tight @13:33:25.67 Elbow.seek PC @13:33:25.79 Elbow.reconfigure :loose @13:33:25.89 Forearm.seek PC @13:33:26.83 Hand.close @13:33:27.44 PC.seek :open @13:33:32.16 Forearm.seek :retract @13:33:33.11 Elbow.reconfigure :tight @13:33:33.16 Elbow.seek :garage @13:33:34.48 Elbow.reconfigure :loose @13:33:34.58 Forearm.reconfigure :tight @13:33:34.63 Forearm.seek :garage @13:33:35.70 Hand.open @13:33:36.37 Forearm.reconfigure :loose @13:33:36.42 Forearm.seek :retract @15:49:40.16 Session Begun @15:49:45.36 -> FLush @15:49:45.38 NameError in dave -- uninitialized constant FLush @15:49:47.82 -> Flush FlushPuck is in FlushPuck::Garage @15:49:49.92 -> CC Collection Clamp contains wcr puck from tube 7 @15:49:52.16 -> PC Processing Clamp open @15:50:11.38 -> CC.unloadPuck 6, :WCR @15:50:11.46 Puck::Warning in dave -- Unloading Puck.from 7, :wcr to Storage Carousel @15:50:11.49 Storing WCR puck in Collection Clamp to tube 6 @15:50:11.69 SC.reconfigure :tight @15:50:11.76 SC.seek 6 @15:50:11.84 SC.configure :limp @15:50:12.07 Elbow.reconfigure :tight @15:50:12.15 Elbow.seek 6 @15:50:14.01 Forearm.reconfigure :tight @15:50:14.09 Forearm.seek 6 + 450 counts @15:50:15.14 SE.reconfigure :pick @15:50:15.23 SE.seek :beamBreak @15:50:17.25 SE.configure :default @15:50:17.44 Elbow.reconfigure :limp @15:50:17.50 Forearm.reconfigure :loose @15:50:17.57 Forearm.seek 6 @15:50:17.86 Elbow.hold @15:50:17.95 Elbow.reconfigure :tight @15:50:18.06 SE.seek 17.12 Pucks below Top Plate @15:50:18.65 Forearm.force -20 @15:50:18.71 Forearm.seek 6 + 274 counts @15:50:19.02 Forearm.reconfigure :tight @15:50:19.09 Forearm.seek 6 + 450 counts @15:50:19.31 SE.seek 18.12 Pucks below Top Plate @15:50:19.90 Forearm.reconfigure :loose @15:50:19.97 Forearm.seek :retract @15:50:20.98 Elbow.seek CC @15:50:22.65 Elbow.reconfigure :loose @15:50:22.76 Forearm.seek CC @15:50:23.78 Hand.close @15:50:24.46 CC.seek :open @15:50:29.24 Forearm.seek :retract @15:50:30.24 Elbow.reconfigure :tight @15:50:30.31 Elbow.seek 6 @15:50:32.47 Elbow.reconfigure :limp @15:50:32.61 Forearm.seek 6 @15:50:33.66 Elbow.hold @15:50:33.75 Elbow.reconfigure :tight @15:50:33.82 Hand.open @15:50:34.59 Forearm.force -20 @15:50:34.64 Forearm.seek 6 + 319 counts @15:50:34.97 Forearm.reconfigure :tight @15:50:35.04 Forearm.seek 6 + 450 counts @15:50:35.31 SE.seek :down @15:50:36.44 Forearm.reconfigure :loose @15:50:36.51 Forearm.seek :retract @15:50:36.80 SE.stop @15:51:11.10 -> Flush FlushPuck is in FlushPuck::Garage @15:55:10.43 -> abort! 'mission complete' @15:55:10.45 CmdServer::Client::Error in dave -- Aborting Mission: mission complete @15:55:10.49 BadNews.email "mission complete",Subject: "DA_WCR_122123 ABORTED!" # @15:55:15.25 upsync @15:55:15.34 Session Ended + ESPwhich DA_WCR_122223.rb + fn=DA_WCR_122223.rb + IFS=: + set /home/esp/esp2/mission . + fullPath=/home/esp/esp2/mission/DA_WCR_122223.rb + [ -f /home/esp/esp2/mission/DA_WCR_122223.rb ] + realpath /home/esp/esp2/mission/DA_WCR_122223.rb + return 0 + scriptPath=/home/esp/esp2local/mission/DA_WCR_122223.rb + exec ruby /home/esp/esp2/lib/esp.rb /home/esp/esp2local/mission/DA_WCR_122223.rb @13:28:46.23UTC22-Dec-23
ESP2 v3.42 I2C Gateway v3.20 for core bus @13:28:49.00 Configuration for short puck jake 1/11/21 by brent@mbari.org /home/esp/esp2/type/shallow/jake/configure.rb @13:28:52.71 Polling Can every 10:00 @13:28:52.99 Cleanly exited at 13:25:33UTC22-Dec-23 @13:28:53.04 Loading /home/esp/esp2local/mission/DA_WCR_122223.rb mission @13:28:53.09 GoodNews.email "Starting DA_WCR_122223 w/pucks in tube 7\n{1 => 33,\n 2 => 33,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 19,\n 7 => 12}\nCTD data unavailable\nCan@13:28:52, 24% flow, 20.4C, 38% humidity, 15.4psia, 13.666V, 0.284A, 0.047Ah, 3.89W",Subject: "DA_WCR_122223 begun" @13:28:53.12
Preparing to sleep until + 00:00:05 @13:28:53.18 Asleep til + 00:00:05 @13:28:53.19 Continuing at 13:28 @13:28:53.48 Gate.power :core,:OFF @13:28:53.49 Gate.power :raw,:OFF @13:28:53.50 Shutdown @13:28:56.81 GoodNews.email "Then, in tube 7: initialPurge; da [100, 100] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_122223.rb:14\n{1 => 33,\n 2 => 33,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 19,\n 7 => 12}\nCTD data unavailable\nCan@13:28:53, 24% flow, 20.4C, 38% humidity, 15.4psia, 13.666V, 0.290A, 0.047Ah, 3.97W",Subject: "Asleep until 13:28" @13:28:58.11
Powering core bus @13:28:58.43 upsync @13:29:01.88 upsync @13:29:03.77
Gate.power :raw,:ON @13:29:04.79 CS.configure :default @13:29:04.84 PS.configure :default @13:29:04.89 SS.configure :default @13:29:04.94 CC.configure :default @13:29:04.99 PC.configure :default @13:29:05.06 Forearm.configure :loose @13:29:05.11 Elbow.configure :loose @13:29:05.18 SC.configure :loose @13:29:05.22 SE.configure :default @13:29:05.27 CH.configure :default @13:29:05.31 PH.configure :default @13:29:05.35 All dwarf controllers running firmware version 3.85 @13:29:05.46 Valve.reset @13:29:05.69 Intake.select :close @13:29:05.75 Exhaust.select :close @13:29:05.79 CTV.dial :air @13:29:05.89 PSV.dial :air @13:29:05.91 CSV.dial :waste1 @13:29:06.11 SSV.dial :spit,avoiding: :exhaust @13:29:06.13
Forearm.home.seek :retract @13:29:06.16 PS.home.seek 0ml @13:29:06.42 CS.reconfigure :fast @13:29:06.46 SS.home.seek 0ml @13:29:06.54 CS.home.seek 0ml @13:29:06.90 Hand.close @13:29:06.95
Elbow.reconfigure :loose @13:29:07.03 Elbow.jog 1500 @13:29:07.53 Elbow.reconfigure :loose @13:29:07.60 Elbow.home.seek :clear @13:29:10.83 SE.home.seek :down @13:29:11.46 SC.reconfigure :tight @13:29:11.51 SC.home.seek :initial @13:29:14.72 PS.stop @13:29:30.36
SC.configure :limp @13:29:30.42 Forearm.seek :retract @13:29:30.48 Elbow.seek :hammer @13:29:31.53 Forearm.reconfigure :tight @13:29:31.58 Forearm.seek retracted @13:29:32.24 PC.home.seek :open @13:29:32.26
CC.home.seek :open @13:29:35.62 Phase [initialPurge; da [100, 100] #da and wsr are run simultanelously] @13:29:35.63 Notice.email "At 13:29:35 began in tube 7: initialPurge; da [100, 100] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_122223.rb:14\n{1 => 33,\n 2 => 33,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 19,\n 7 => 12}\nCTD data unavailable\nCan@13:29:35, 24% flow, 20.4C, 38% humidity, 15.4psia, 13.588V, 0.296A, 0.051Ah, 4.03W",Subject: "Started DA_WCR_122223 phase initialPurge; da [100, 100] #da and wsr are run simultanelously" @13:29:35.65
Starting initialPurge @13:29:35.68 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @13:29:35.75 Hand.open @13:29:36.54 Elbow.reconfigure :tight @13:29:36.58 Elbow.seek FlushPuck::Garage @13:29:38.38 Elbow.reconfigure :loose @13:29:38.50 Forearm.reconfigure :loose @13:29:38.55 Forearm.seek :garage @13:29:39.52 Hand.close @13:29:39.96 upsync @13:29:40.30
Forearm.seek :retract @13:29:41.30 Elbow.reconfigure :tight @13:29:41.35 Elbow.seek CC @13:29:44.26 Elbow.reconfigure :loose @13:29:44.36 Forearm.reconfigure :tight @13:29:44.41 Forearm.seek CC @13:29:45.71 CC.seek :closed @13:29:50.25 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.41 @13:29:50.32 Hand.open @13:29:50.99 Forearm.reconfigure :loose @13:29:51.04 Forearm.seek :retract @13:29:52.06 Flushing the Collection Valve::Manifold @13:29:52.18 CSV.dial CSR @13:29:53.48 Pulling 5ml of CSR.flush CSR.select :flush @13:29:53.60 CS.reconfigure :default @13:29:53.65 CS.seek 5ml @13:30:07.87 delay 5 seconds @13:30:12.88 CSR.select :air @13:30:13.06 CS.reconfigure :fast @13:30:13.11 CS.seek 7.5ml @13:30:19.87 CSV.dial :waste @13:30:21.15 CS.reconfigure :default @13:30:21.20 CS.seek 0ml @13:30:41.21 Purging external Intake @13:30:41.33 CSV.dial :flush @13:30:41.79 CS.reconfigure :fast @13:30:41.84 CS.seek 10ml @13:31:06.46 delay 5 seconds @13:31:11.48 CSV.dial CBV @13:31:11.52
CBV.dial CSV @13:31:12.29 CTV.dial :intake @13:31:13.08 Intake.select :open @13:31:13.20 CS.seek 0ml @13:31:36.83 Intake.select :close @13:31:36.94 CTV.dial :air @13:31:37.71 Flushing upper manifold @13:31:37.84 CSV.dial :flush @13:31:38.58 CS.seek 5ml @13:31:51.55 delay 5 seconds @13:31:56.57 CSV.dial :air @13:31:57.10 CS.seek 7ml @13:32:02.87 CSV.dial CBV @13:32:02.89
CBV.dial CSV @13:32:03.93 CTV.dial :air @13:32:04.01 CS.seek 0ml @13:32:20.65 delay 7 seconds @13:32:27.67 CS.seek 3ml @13:32:35.74 CSV.dial :waste @13:32:36.20 CS.seek 0ml @13:32:44.13 CSV.dial :flush @13:32:44.59 CS.seek 3ml @13:32:52.64 delay 5 seconds @13:32:57.66 CSV.dial :air @13:32:58.18 CS.seek 5ml @13:33:03.92 CSV.dial :air @13:33:04.10 CS.seek 0ml @13:33:16.24 delay 7 seconds @13:33:23.27 CS.seek 3ml @13:33:31.33 CSV.dial :waste @13:33:32.07 CS.seek 0ml @13:33:39.80 Garaging FlushPuck in Collection Clamp @13:33:39.87 Elbow.reconfigure :tight @13:33:39.92 Elbow.seek CC @13:33:40.16 Elbow.reconfigure :loose @13:33:40.26 Forearm.seek CC @13:33:41.19 Hand.close @13:33:41.79 CC.seek :open @13:33:46.85 Forearm.seek :retract @13:33:47.88 Elbow.reconfigure :tight @13:33:47.93 Elbow.seek :garage @13:33:50.85 Elbow.reconfigure :loose @13:33:50.95 Forearm.reconfigure :tight @13:33:50.99 Forearm.seek :garage @13:33:52.04 Hand.open @13:33:52.72 Forearm.reconfigure :loose @13:33:52.77 Forearm.seek :retract @13:33:53.76 da sampling at most 100ml, wcr at most 100ml @13:33:53.80 Loading sh1 puck from tube 7 into Collection Clamp @13:33:53.81 DAfans.email "Started da in tube 7 at 13:33:53UTC22-Dec-23\n{1 => 33,\n 2 => 33,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 19,\n 7 => 12}\nCTD data unavailable\nCan@13:29:35, 24% flow, 20.4C, 38% humidity, 15.4psia, 13.588V, 0.296A, 0.051Ah, 4.03W",Subject: "da sampling at most 100ml, wcr at most 100ml" @13:33:54.09
SC.reconfigure :tight @13:33:54.16 SC.seek 7 @13:33:58.15 upsync @13:34:03.97
SC.configure :limp @13:34:04.21 Elbow.reconfigure :tight @13:34:04.26 Elbow.seek 7 @13:34:06.20 Forearm.reconfigure :tight @13:34:06.25 Forearm.seek 7 + 450 counts @13:34:07.29 SE.reconfigure :pick @13:34:07.35 SE.seek :beamBreak @13:34:09.82 SE.configure :default @13:34:09.99 Elbow.seek 7 @13:34:10.19 Forearm.seek 7 + 300 counts @13:34:10.43 Forearm.reconfigure :loose @13:34:10.48 Hand.close @13:34:11.05 SE.seek :down @13:34:11.26
Forearm.seek :retract @13:34:12.63 Elbow.seek CC @13:34:13.16 SE.stop @13:34:14.25
Elbow.reconfigure :loose @13:34:14.35 Forearm.reconfigure :tight @13:34:14.40 Forearm.seek CC @13:34:15.68 CC.seek :closed @13:34:20.21 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.30 @13:34:20.22 Hand.open @13:34:20.85 Forearm.reconfigure :loose @13:34:20.90 Forearm.seek :retract @13:34:21.93 collectSample 100ml w/bubble point of 24psi @13:34:21.94 Priming 7ml @13:34:21.95 Intake.select :close @13:34:21.96 Exhaust.select :close @13:34:21.98 SS.hold @13:34:22.19 Calibrating pressure and temperature sensors @13:34:22.22 CTV.dial :air @13:34:22.23 SSV.dial :spit,avoiding: :exhaust @13:34:22.32 SS.reconfigure :fast @13:34:22.37 SS.seek 5ml @13:34:25.30 SSV.dial CBV @13:34:25.32
CBV.dial SSV @13:34:26.69 delay 3.8 seconds @13:34:30.61 Sensor limits: Intake max 96.1psia; Outlet max 92.8psia @13:34:30.62 SSV.dial :spit @13:34:31.94 SS.seek 0ml @13:34:34.84 SSV.dial CBV @13:34:36.19 CTV.dial :intake @13:34:36.98 SSV.dial CBV @13:34:37.00
CBV.dial SSV @13:34:37.22 Allowing 40s for 7.0ml of remaining 7.0ml @13:34:37.23 Intake.select :open @13:34:39.39 Intake @15.4psia, vacuum from 9.9 to 12.9psi @13:34:39.41 SS.reconfigure :inhaling 2.20ml/s @13:34:39.46 SS.seek 7ml @13:34:46.62 Stroke completed -- waiting for pressure to equalize ... @13:34:46.63 SS.reconfigure :exhale @13:34:46.68 SS.hold @13:34:49.84 Intake.select :close @13:34:50.00 SSV.dial :exhaust @13:34:50.76 Exhaust.select :open @13:34:50.87 SS.seek 0ml @13:34:55.04 Exhaust.select :close @13:34:55.16 Sampling 100.0ml @13:34:55.17 Intake.select :close @13:34:55.18 Exhaust.select :close @13:34:55.19 SS.reconfigure :default @13:34:55.29 SS.hold @13:34:55.54 CTV.dial :intake @13:34:55.61 SSV.dial CBV @13:34:55.65
CBV.dial SSV @13:34:56.40 Allowing 130s for 25.0ml of remaining 100.0ml @13:34:56.42 Intake.select :open @13:34:58.58 Intake @15.6psia, vacuum from 10.0 to 13.0psi @13:34:58.60 SS.reconfigure :inhaling 2.20ml/s @13:34:58.65 SS.seek 25ml @13:35:12.96 Vacuum too high -- waiting up to 40 seconds @13:35:12.97 SS.reconfigure :exhale @13:35:13.02 SS.hold @13:35:13.71 Intake @11.6psia, vacuum from 6.1 to 9.1psi @13:35:13.73 SS.reconfigure :inhaling 1.76ml/s @13:35:13.78 SS.seek 25ml @13:35:16.06 Stroke completed -- waiting for pressure to equalize ... @13:35:16.07 SS.reconfigure :exhale @13:35:16.12 SS.hold @13:35:20.88 Intake.select :close @13:35:21.04 SSV.dial :exhaust @13:35:21.81 Exhaust.select :open @13:35:21.92 SS.seek 0ml @13:35:30.82 Exhaust.select :close @13:35:30.93 SSV.dial CBV @13:35:30.97
CBV.dial SSV @13:35:31.72 Allowing 130s for 25.0ml of remaining 75.0ml @13:35:31.73 Intake.select :open @13:35:34.32 Intake @15.6psia, vacuum from 10.0 to 13.0psi @13:35:34.33 SS.reconfigure :inhaling 1.76ml/s @13:35:34.38 SS.seek 25ml @13:35:52.28 Stroke completed -- waiting for pressure to equalize ... @13:35:52.29 SS.reconfigure :exhale @13:35:52.33 SS.hold @13:35:57.97 Intake.select :close @13:35:58.12 SSV.dial :exhaust @13:35:58.87 Exhaust.select :open @13:35:58.99 SS.seek 0ml @13:36:07.81 Exhaust.select :close @13:36:07.92 SSV.dial CBV @13:36:07.94
CBV.dial SSV @13:36:08.72 Allowing 130s for 25.0ml of remaining 50.0ml @13:36:08.73 Intake.select :open @13:36:10.89 Intake @15.5psia, vacuum from 10.0 to 13.0psi @13:36:10.90 SS.reconfigure :inhaling 1.76ml/s @13:36:10.95 SS.seek 25ml @13:36:29.17 Stroke completed -- waiting for pressure to equalize ... @13:36:29.18 SS.reconfigure :exhale @13:36:29.23 SS.hold @13:36:34.92 Intake.select :close @13:36:35.08 SSV.dial :exhaust @13:36:35.84 Exhaust.select :open @13:36:35.95 SS.seek 0ml @13:36:44.92 Exhaust.select :close @13:36:45.03 SSV.dial CBV @13:36:45.05
CBV.dial SSV @13:36:45.82 Allowing 130s for 25.0ml of remaining 25.0ml @13:36:45.84 Intake.select :open @13:36:48.00 Intake @15.5psia, vacuum from 10.0 to 13.0psi @13:36:48.01 SS.reconfigure :inhaling 1.76ml/s @13:36:48.06 SS.seek 24.99ml @13:37:06.30 Stroke completed -- waiting for pressure to equalize ... @13:37:06.31 SS.reconfigure :exhale @13:37:06.36 SS.hold @13:37:12.85 Intake.select :close @13:37:13.00 SSV.dial :exhaust @13:37:13.76 Exhaust.select :open @13:37:13.88 SS.seek 0ml @13:37:22.77 Exhaust.select :close @13:37:22.88 Sampled 100.0ml @13:37:22.90 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) Intake.select :close @13:37:22.97 CSV.dial :waste @13:37:23.10 SSV.dial CBV,avoiding: :exhaust @13:37:23.13
CBV.dial SSV @13:37:23.21 CS.seek 0ml @13:37:23.30 CSV.dial :air @13:37:24.04 CS.seek 10ml @13:37:25.06
CTV.dial :air @13:37:25.86 delay 15 seconds @13:37:40.88 SSV.dial :spit,avoiding: :exhaust @13:37:42.22 CTV.dial CSV,avoiding: :intake @13:37:42.25
SS.seek 0ml @13:37:42.39 SSV.dial CBV,avoiding: :exhaust @13:37:48.80 SSV.dial between CBV and plug @13:37:49.72 CBV.dial between SSV and waste2 @13:37:50.11 CTV.dial CSV,avoiding: :air @13:37:50.14
CSV.dial CTV @13:37:50.79 CS.reconfigure :pressurize @13:37:50.84 CS.seek 0ml @13:38:08.93 BubblePoint: Intake @24.3psia, Outlet @0.7psia @13:38:08.94 CBV.dial SSV @13:38:09.28 SS.reconfigure :default @13:38:09.33 SS.seek 10ml @13:38:15.12 delay 15 seconds @13:38:30.25 Evacuated: Intake @24.0psia, Outlet @24.2psia @13:38:30.26 SSV.dial :spit,avoiding: :exhaust @13:38:31.31 SS.reconfigure :exhale @13:38:31.36 SS.seek 0ml @13:38:36.74 SS.reconfigure :fast @13:38:36.79 SS.seek 5ml @13:38:39.78 SSV.dial CBV,avoiding: :exhaust @13:38:39.80
CBV.dial SSV @13:38:41.18 CTV.dial :air,avoiding: :intake @13:38:42.52 CSV.dial CTV @13:38:42.55
SS.seek 10ml @13:38:43.68 CSV.dial :air @13:38:44.14 CS.reconfigure :fast @13:38:44.19 CS.seek 0ml @13:38:45.21
SSV.dial :spit,avoiding: :exhaust @13:38:46.56 SS.reconfigure :exhale @13:38:46.61 SS.seek 0ml @13:38:52.05 SS.reconfigure :fast @13:38:52.10 SS.seek 5ml @13:38:52.17 CS.stop @13:38:55.00 SSV.dial CBV,avoiding: :exhaust @13:38:55.02
CBV.dial SSV @13:38:57.39 SSV.dial :spit,avoiding: :exhaust @13:38:58.73 SS.reconfigure :default @13:38:58.78 SS.seek 0ml @13:39:02.26 SS.stop @13:39:02.30 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @13:39:02.31 Intake.select :close @13:39:02.38 CSV.dial :waste @13:39:02.53 SSV.dial CBV,avoiding: :exhaust @13:39:02.55
CBV.dial SSV @13:39:03.16 CS.seek 0ml @13:39:03.23 CSV.dial :air @13:39:03.93
CTV.dial :air @13:39:04.01 CS.seek 10ml @13:39:04.08
delay 15 seconds @13:39:19.10 SSV.dial :spit,avoiding: :exhaust @13:39:20.43 CTV.dial CSV,avoiding: :intake @13:39:20.47
SS.reconfigure :exhale @13:39:20.57 SS.seek 0ml @13:39:20.99 SSV.dial CBV,avoiding: :exhaust @13:39:28.55 SSV.dial between CBV and plug @13:39:29.45 CBV.dial between SSV and waste2 @13:39:29.86 CSV.dial between air and CTV @13:39:30.16 CS.seek 8.279ml @13:39:35.02 CTV.dial CSV,avoiding: :air @13:39:35.04
CSV.dial CTV @13:39:35.41 CS.reconfigure :pressurize @13:39:35.46 CS.seek 0ml @13:39:53.36 BubblePoint: Intake @24.2psia, Outlet @0.6psia @13:39:53.37 CBV.dial SSV @13:39:53.72 SS.reconfigure :default @13:39:53.77 SS.seek 10ml @13:39:59.48 delay 15 seconds @13:40:14.61 Evacuated: Intake @23.8psia, Outlet @17.6psia @13:40:14.62 SSV.dial :spit,avoiding: :exhaust @13:40:15.65 SS.reconfigure :exhale @13:40:15.70 SS.seek 0ml @13:40:21.19 SS.reconfigure :fast @13:40:21.24 SS.seek 5ml @13:40:24.40 SSV.dial CBV,avoiding: :exhaust @13:40:24.43
CBV.dial SSV @13:40:25.78 CTV.dial :air,avoiding: :intake @13:40:27.12 CSV.dial CTV @13:40:27.18
SS.seek 10ml @13:40:28.33 CSV.dial :air @13:40:28.78 CS.reconfigure :fast @13:40:28.83 CS.seek 0ml @13:40:29.90
SSV.dial :spit,avoiding: :exhaust @13:40:31.24 SS.reconfigure :exhale @13:40:31.29 SS.seek 0ml @13:40:33.07 CS.stop @13:40:36.72
SS.reconfigure :fast @13:40:36.77 SS.seek 5ml @13:40:39.70 SSV.dial CBV,avoiding: :exhaust @13:40:39.72
CBV.dial SSV @13:40:42.09 SSV.dial :spit,avoiding: :exhaust @13:40:43.42 SS.reconfigure :default @13:40:43.47 SS.seek 0ml @13:40:46.89 SS.stop @13:40:46.94 Lysing: Adding 2.0ml AqMeOH @13:40:46.95 CBV.dial between CSV and waste1,avoiding: :DBV @13:40:48.45 CTV.dial CSV @13:40:49.76 CSV.dial CSR @13:40:50.55 Pulling 1ml of CSR.AqMeOH @13:40:50.56 CSR.select :AqMeOH @13:40:50.67 CS.reconfigure :default @13:40:50.72 CS.seek 1ml @13:40:54.10 delay 5 seconds @13:40:59.12 CSR.select :air @13:40:59.29 CS.seek 6ml @13:41:12.92 delay 2 seconds @13:41:15.31 CSV.dial :waste2 @13:41:15.81 CS.reconfigure :fast @13:41:15.86 CS.seek 0ml @13:41:30.17 CSV.dial CSR @13:41:30.62 Pulling 2ml of CSR.AqMeOH @13:41:30.63 CSR.select :AqMeOH @13:41:30.74 CS.reconfigure :default @13:41:30.79 CS.seek 2ml @13:41:37.07 delay 5 seconds @13:41:42.09 CSR.select :air @13:41:42.26 CS.seek 5ml @13:41:50.95 delay 2 seconds @13:41:52.96 CSV.dial CTV @13:41:53.41 CS.seek 1ml @13:42:04.77 Before lysing Collection Puck Heater at 20.2C/25.7psia @13:42:04.79 Heating Puck.from 7, :sh1 in Collection Clamp to 70C for 10:00 @13:42:04.81 CH.seek 70C,8:00 @13:44:09.14 CH.hold 10:00,70C @13:44:09.34 Begun lysing Collection Puck Heater at 68C/31.6psia @13:54:09.34 Ended lysing Collection Puck Heater at 70C/30.2psia @13:54:09.36 CS.seek 0ml @13:54:12.75 CTV.dial between spare and air,avoiding: :intake @13:54:13.79 CSV.dial :air @13:54:14.26 CS.seek 5ml @13:54:28.09 CSV.dial CTV @13:54:28.67 delay 2 seconds @13:54:30.69 CSV.dial :air @13:54:31.12 delay 2 seconds @13:54:33.14 CSV.dial CTV @13:54:33.59 delay 2 seconds @13:54:35.61 CSV.dial :air @13:54:36.06 delay 2 seconds @13:54:38.08 CS.seek 0ml @13:54:51.74 Puck @60.4psia after final lysis pressurization @13:54:51.75 Recovering Lysate into Collection syringe @13:54:51.76 CSV.dial CBV @13:54:52.79 CBV.dial CSV @13:54:53.18 CS.seek 5ml @13:55:07.00 delay 5 seconds @13:55:12.01 CTV.dial :air @13:55:12.54 CS.seek 7.001ml @13:55:18.53 delay 5 seconds @13:55:23.55 CSV.dial :air2 @13:55:23.99 CS.seek 10ml @13:55:32.44 CSV.dial between air and CTV,avoiding: CBV @13:55:33.61 Lysing Complete @13:55:33.68 Storing sh1 puck in Collection Clamp to tube 6 @13:55:33.85 SC.reconfigure :tight @13:55:33.90 SC.seek 6 @13:55:37.42 SC.configure :limp @13:55:37.65 Elbow.reconfigure :tight @13:55:38.14 Elbow.seek 6 @13:55:39.84 Forearm.reconfigure :tight @13:55:39.89 Forearm.seek 6 + 450 counts @13:55:40.91 SE.reconfigure :pick @13:55:40.97 SE.seek :beamBreak @13:55:42.82 SE.configure :default @13:55:42.98 Elbow.reconfigure :limp @13:55:43.03 Forearm.reconfigure :loose @13:55:43.07 Forearm.seek 6 @13:55:43.35 Elbow.hold @13:55:44.03 Elbow.reconfigure :tight @13:55:44.13 SE.seek 18.11 Pucks below Top Plate @13:55:44.55 Forearm.force -20 @13:55:44.59 Forearm.seek 6 + 304 counts @13:55:44.89 Forearm.reconfigure :tight @13:55:44.94 Forearm.seek 6 + 450 counts @13:55:45.13 SE.seek 19.11 Pucks below Top Plate @13:55:45.68 Forearm.reconfigure :loose @13:55:45.73 Forearm.seek :retract @13:55:46.71 Elbow.seek CC @13:55:48.32 Elbow.reconfigure :loose @13:55:48.42 Forearm.seek CC @13:55:49.39 Hand.close @13:55:50.02 CC.seek :open @13:55:54.79 Forearm.seek :retract @13:55:55.77 Elbow.reconfigure :tight @13:55:55.82 Elbow.seek 6 @13:55:57.81 Elbow.reconfigure :limp @13:55:57.86 Forearm.seek 6 @13:55:58.85 Elbow.hold @13:55:58.91 Elbow.reconfigure :tight @13:55:58.96 Hand.open @13:56:00.10 Forearm.force -20 @13:56:00.14 Forearm.seek 6 + 312 counts @13:56:00.44 Forearm.reconfigure :tight @13:56:00.49 Forearm.seek 6 + 450 counts @13:56:00.76 SE.seek :down @13:56:01.89
Forearm.reconfigure :loose @13:56:01.94 Forearm.seek :retract @13:56:02.14 SE.stop @13:56:02.87
Tranferring DA @13:56:02.88 Beginning samplePassC @13:56:02.89 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @13:56:03.08 Elbow.seek FlushPuck::Garage @13:56:04.96 Elbow.reconfigure :loose @13:56:05.06 Forearm.seek :garage @13:56:06.10 Hand.close @13:56:06.89 Forearm.seek :retract @13:56:07.86 Elbow.reconfigure :tight @13:56:07.91 Elbow.seek CC @13:56:10.79 Elbow.reconfigure :loose @13:56:10.90 Forearm.reconfigure :tight @13:56:10.94 Forearm.seek CC @13:56:12.25 CC.seek :closed @13:56:16.78 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.37 @13:56:16.85 Hand.open @13:56:17.96 Forearm.reconfigure :loose @13:56:18.01 Forearm.seek :retract @13:56:18.99 PSV.dial PSR @13:56:20.39 Pulling 1.5ml of PSR.flush @13:56:20.40 PSR.select :flush @13:56:20.53 PS.seek 1.5ml @13:56:36.69 delay 5 seconds @13:56:41.71 PSV.dial :waste1 @13:56:42.18 PS.seek 0ml @13:56:58.35 PSV.dial PSR @13:56:58.82 PSR.select :air @13:56:58.94 PS.seek 2.5ml @13:57:25.45 PRV.dial CBV,avoiding: :ab2 @13:57:25.47
CBV.dial PRV @13:57:27.24 PSV.dial :mixloop @13:57:28.29 CTV.dial :air,avoiding: :intake @13:57:28.46 PTV.dial :PRVmixing @13:57:29.79 PS.seek 0ml @13:57:56.10 PSV.dial :air @13:57:56.68 PS.seek 2.5ml @13:58:23.00 PSV.dial :mixloop @13:58:23.46 PS.seek 0ml @13:58:49.81 PSV.dial :air @13:58:50.29 PS.seek 2.5ml @13:59:16.59 PSV.dial :mixloop @13:59:17.07 PS.seek 0ml @13:59:43.41 Garaging FlushPuck in Collection Clamp @13:59:43.48 Elbow.reconfigure :tight @13:59:43.53 Elbow.seek CC @13:59:43.81 Elbow.reconfigure :loose @13:59:43.90 Forearm.seek CC @13:59:44.97 Hand.close @13:59:45.64 CC.seek :open @13:59:50.30 Forearm.seek :retract @13:59:51.30 Elbow.reconfigure :tight @13:59:51.35 Elbow.seek :garage @13:59:54.76 Elbow.reconfigure :loose @13:59:54.86 Forearm.reconfigure :tight @13:59:54.91 Forearm.seek :garage @13:59:55.98 Hand.open @13:59:56.70 Forearm.reconfigure :loose @13:59:56.75 Forearm.seek :retract @13:59:57.75 PSV.dial :mixloop @13:59:57.83 PTV.dial :PRVmixing @13:59:57.91 PRV.dial CBV @13:59:57.93
CBV.dial PRV @13:59:58.15 CTV.dial :air,avoiding: :intake @13:59:58.17 Loading lyfil puck from tube 7 into Collection Clamp @13:59:58.41 SC.reconfigure :tight @13:59:58.45 SC.seek 7 @14:00:02.04 SC.configure :limp @14:00:02.26 Elbow.reconfigure :tight @14:00:02.31 Elbow.seek 7 @14:00:04.28 Forearm.reconfigure :tight @14:00:04.32 Forearm.seek 7 + 450 counts @14:00:05.43 SE.reconfigure :pick @14:00:05.49 SE.seek :beamBreak @14:00:07.91 SE.configure :default @14:00:08.07 Elbow.seek 7 @14:00:08.25 Forearm.seek 7 + 300 counts @14:00:08.42 Forearm.reconfigure :loose @14:00:08.47 Hand.close @14:00:09.16 SE.seek :down @14:00:09.36
Forearm.seek :retract @14:00:10.31 Elbow.seek CC @14:00:11.24 SE.stop @14:00:12.40
Elbow.reconfigure :loose @14:00:12.50 Forearm.reconfigure :tight @14:00:12.55 Forearm.seek CC @14:00:13.77 CC.seek :closed @14:00:18.28 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.22 @14:00:18.30 Hand.open @14:00:18.95 Forearm.reconfigure :loose @14:00:19.00 Forearm.seek :retract @14:00:19.97 CTV.dial CSV,avoiding: :intake @14:00:22.30 CSV.dial CTV @14:00:22.64 PS.seek 2.5ml @14:00:49.13 CS.seek 4.5ml @14:01:04.08 delay 8 seconds @14:01:12.10 PSV.dial :air @14:01:12.56 delay 2 seconds @14:01:14.58 PSV.dial :waste2 @14:01:15.32 PS.seek 0ml @14:01:41.75 CBV.dial between PRV and waste1 @14:01:42.08 CSV.dial :air @14:01:42.54 CS.seek 10ml @14:01:57.55 CSV.dial CTV @14:01:58.01 CS.seek 0ml @14:02:24.80 CBV.dial :waste1 @14:02:25.12 delay 5 seconds @14:02:30.14 CTV.dial :air,avoiding: :intake @14:02:31.55 Storing lyfil puck in Collection Clamp to tube 6 @14:02:31.73 SC.reconfigure :tight @14:02:32.15 SC.seek 6 @14:02:35.82 SC.configure :limp @14:02:36.04 Elbow.reconfigure :tight @14:02:36.09 Elbow.seek 6 @14:02:37.87 Forearm.reconfigure :tight @14:02:37.92 Forearm.seek 6 + 450 counts @14:02:39.12 SE.reconfigure :pick @14:02:39.18 SE.seek :beamBreak @14:02:41.02 SE.configure :default @14:02:41.19 Elbow.reconfigure :limp @14:02:41.24 Forearm.reconfigure :loose @14:02:41.28 Forearm.seek 6 @14:02:41.59 Elbow.hold @14:02:41.65 Elbow.reconfigure :tight @14:02:41.76 SE.seek 19.11 Pucks below Top Plate @14:02:42.28 Forearm.force -20 @14:02:42.32 Forearm.seek 6 + 298 counts @14:02:42.62 Forearm.reconfigure :tight @14:02:42.67 Forearm.seek 6 + 450 counts @14:02:42.86 SE.seek 20.11 Pucks below Top Plate @14:02:43.44 Forearm.reconfigure :loose @14:02:43.49 Forearm.seek :retract @14:02:44.41 Elbow.seek CC @14:02:46.08 Elbow.reconfigure :loose @14:02:46.18 Forearm.seek CC @14:02:47.15 Hand.close @14:02:47.77 CC.seek :open @14:02:52.98 Forearm.seek :retract @14:02:53.95 Elbow.reconfigure :tight @14:02:54.00 Elbow.seek 6 @14:02:55.77 Elbow.reconfigure :limp @14:02:55.82 Forearm.seek 6 @14:02:56.79 Elbow.hold @14:02:56.86 Elbow.reconfigure :tight @14:02:56.91 Hand.open @14:02:58.24 Forearm.force -20 @14:02:58.28 Forearm.seek 6 + 320 counts @14:02:58.58 Forearm.reconfigure :tight @14:02:58.63 Forearm.seek 6 + 450 counts @14:02:58.91 SE.seek :down @14:03:00.04
Forearm.reconfigure :loose @14:03:00.09 Forearm.seek :retract @14:03:00.22 SE.stop @14:03:01.01
PSV.dial :air @14:03:01.77 PS.seek 2.5ml @14:03:28.15 delay 1 second @14:03:29.17 PSV.dial :mixloop @14:03:29.62 delay 1 second @14:03:30.64 PSV.dial :air @14:03:31.11 delay 1 second @14:03:32.12 PSV.dial :mixloop @14:03:32.59 delay 1 second @14:03:33.61 PSV.dial :air @14:03:34.08 delay 1 second @14:03:35.10 PS.seek 0ml @14:04:01.44 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @14:04:01.62 Elbow.seek FlushPuck::Garage @14:04:03.41 Elbow.reconfigure :loose @14:04:03.51 Forearm.seek :garage @14:04:04.56 Hand.close @14:04:05.76 Forearm.seek :retract @14:04:06.77 Elbow.reconfigure :tight @14:04:06.81 Elbow.seek PC @14:04:08.08 Elbow.reconfigure :loose @14:04:08.18 Forearm.reconfigure :tight @14:04:08.23 Forearm.seek PC @14:04:09.55 PC.seek :closed @14:04:14.19 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.66 @14:04:14.26 Hand.open @14:04:14.95 Forearm.reconfigure :loose @14:04:15.00 Forearm.seek :retract @14:04:15.96 PRV.dial :air @14:04:16.43 PSV.dial :mixloop @14:04:16.97 PS.seek 0.3ml @14:04:20.84 PSV.dial PSR @14:04:21.91 Pulling 0.4ml of PSR.air @14:04:21.92 PSR.select :air @14:04:21.94 PS.seek 0.7ml @14:04:26.87 delay 3 seconds @14:04:29.89 PSR.select :air @14:04:29.91 PS.seek 2.5ml @14:04:49.09 delay 3 seconds @14:04:52.11 PSV.dial :mixloop @14:04:53.17 PTV.dial :mixing @14:04:53.93 PBV.dial :waste2 @14:04:54.91 PS.seek 0ml @14:05:21.25 Garaging FlushPuck in Processing Clamp @14:05:21.32 Elbow.reconfigure :tight @14:05:21.37 Elbow.seek PC @14:05:21.49 Elbow.reconfigure :loose @14:05:22.03 Forearm.seek PC @14:05:22.99 Hand.close @14:05:23.64 PC.seek :open @14:05:28.37 Forearm.seek :retract @14:05:29.34 Elbow.reconfigure :tight @14:05:29.39 Elbow.seek :garage @14:05:30.74 Elbow.reconfigure :loose @14:05:30.84 Forearm.reconfigure :tight @14:05:30.89 Forearm.seek :garage @14:05:31.95 Hand.open @14:05:32.57 Forearm.reconfigure :loose @14:05:32.62 Forearm.seek :retract @14:05:33.68 Loading da puck from tube 7 into Processing Clamp @14:05:33.91 SC.reconfigure :tight @14:05:33.96 SC.seek 7 @14:05:37.50 SC.configure :limp @14:05:37.72 Elbow.reconfigure :tight @14:05:37.77 Elbow.seek 7 @14:05:39.62 Forearm.reconfigure :tight @14:05:39.67 Forearm.seek 7 + 450 counts @14:05:40.68 SE.reconfigure :pick @14:05:40.74 SE.seek :beamBreak @14:05:43.27 SE.configure :default @14:05:43.44 Elbow.seek 7 @14:05:43.61 Forearm.seek 7 + 300 counts @14:05:43.82 Forearm.reconfigure :loose @14:05:43.87 Hand.close @14:05:44.53 SE.seek :down @14:05:45.18
Forearm.seek :retract @14:05:46.14 Elbow.seek PC @14:05:46.69 SE.stop @14:05:48.88
Elbow.reconfigure :loose @14:05:48.99 Forearm.reconfigure :tight @14:05:49.03 Forearm.seek PC @14:05:50.22 PC.seek :closed @14:05:54.85 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.66 @14:05:54.86 Hand.open @14:05:55.58 Forearm.reconfigure :loose @14:05:55.63 Forearm.seek :retract @14:05:56.57 Heating Puck.from 7, :da in Processing Clamp to 28C for 2:00:00 @14:05:56.58 PH.seek 28C,5:00 @14:06:28.78 PH.hold 2:00:00,28C @14:06:28.83 delay 1:30 @14:07:58.87 PSR.select :air @14:07:58.89 PSV.dial PSR @14:07:59.95 Pulling 0.5ml of PSR.wash PSR.select :wash @14:08:00.08 PS.seek 0.5ml @14:08:06.21 delay 5 seconds @14:08:11.23 PSR.select :air @14:08:11.35 PS.seek 2ml @14:08:27.55 PTV.dial :puck @14:08:28.31 PSV.dial PTV @14:08:29.07 PBV.dial :waste1 @14:08:30.48 PS.seek 1.5ml @14:08:36.49 delay 3 seconds @14:08:39.51 PBV.dial between PSV and waste1 @14:08:39.87 PS.seek 0ml @14:08:56.15 delay 5:00 @14:13:56.17 recoverPSRreagent08 PTV.dial :puck @14:13:56.36 PBV.dial :waste1 @14:13:56.74 delay 30 seconds @14:14:26.76 PBV.dial between PSV and waste1 @14:14:27.14 PSR.select :air @14:14:27.16 PSV.dial PSR @14:14:27.93 PS.reconfigure :fast @14:14:27.98 PS.seek 2.5ml @14:14:52.01 PSV.dial PTV @14:14:52.91 delay 5 seconds @14:14:57.93 PSV.dial PSR @14:14:58.67 delay 5 seconds @14:15:03.69 PSV.dial PTV @14:15:04.45 delay 5 seconds @14:15:09.47 PSV.dial :air @14:15:10.37 PS.seek 0ml @14:15:33.92 PTV.dial :PRVmixing,via: PRV @14:15:35.26 PSV.dial :mixloop @14:15:35.72 PRV.dial :ab1 @14:15:36.49 PS.reconfigure :slow @14:15:36.54 PS.seek 0.15ml @14:15:40.08 delay 2 seconds @14:15:42.10 CBV.dial PRV @14:15:42.12
PRV.dial CBV @14:15:42.70 PS.seek 0.35ml @14:15:47.30 delay 2 seconds @14:15:49.31 CBV.dial between PRV and PSV @14:15:49.65 PRV.dial :air @14:15:50.53 PS.seek 0.9ml @14:16:02.29 PRV.dial :waste2 @14:16:03.05 PS.seek 0.5ml @14:16:11.76 addPMLReagent08 @14:16:11.77 PSV.dial :air @14:16:12.25 PS.reconfigure :default @14:16:12.30 PS.seek 2ml @14:16:28.48 PSV.dial :PML @14:16:28.97 PTV.dial :mixing @14:16:29.72 PBV.dial :waste1 @14:16:30.16 PS.seek 1.85ml @14:16:32.54 PBV.dial between PSV and waste1 @14:16:32.91 PS.seek 0ml @14:16:52.61 delay 2 seconds @14:16:54.63 Beginning samplePassC @14:16:54.64 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:16:54.82 Elbow.reconfigure :tight @14:16:54.87 Elbow.seek FlushPuck::Garage @14:16:56.17 Elbow.reconfigure :loose @14:16:56.27 Forearm.seek :garage @14:16:57.31 Hand.close @14:16:58.00 Forearm.seek :retract @14:16:59.02 Elbow.reconfigure :tight @14:16:59.07 Elbow.seek CC @14:17:01.97 Elbow.reconfigure :loose @14:17:02.07 Forearm.reconfigure :tight @14:17:02.12 Forearm.seek CC @14:17:03.40 CC.seek :closed @14:17:08.53 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.48 @14:17:08.61 Hand.open @14:17:09.31 Forearm.reconfigure :loose @14:17:09.36 Forearm.seek :retract @14:17:10.34 PSV.dial PSR @14:17:11.42 Pulling 1.5ml of PSR.flush @14:17:11.43 PSR.select :flush @14:17:11.55 PS.seek 1.5ml @14:17:27.70 delay 5 seconds @14:17:32.72 PSV.dial :waste1 @14:17:33.32 PS.seek 0ml @14:17:49.48 PSV.dial PSR @14:17:49.95 PSR.select :air @14:17:50.07 PS.seek 2.5ml @14:18:16.43 PRV.dial CBV,avoiding: :ab2 @14:18:16.45
CBV.dial PRV @14:18:18.11 PSV.dial :mixloop @14:18:19.16 CTV.dial :air,avoiding: :intake @14:18:19.24 PTV.dial :PRVmixing @14:18:20.00 PS.seek 0ml @14:18:46.54 PSV.dial :air @14:18:47.13 PS.seek 2.5ml @14:19:13.42 PSV.dial :mixloop @14:19:13.88 PS.seek 0ml @14:19:40.29 PSV.dial :air @14:19:40.89 PS.seek 2.5ml @14:20:07.26 PSV.dial :mixloop @14:20:07.72 PS.seek 0ml @14:20:34.14 Garaging FlushPuck in Collection Clamp @14:20:34.21 Elbow.reconfigure :tight @14:20:34.26 Elbow.seek CC @14:20:34.51 Elbow.reconfigure :loose @14:20:34.61 Forearm.seek CC @14:20:35.56 Hand.close @14:20:36.13 CC.seek :open @14:20:40.78 Forearm.seek :retract @14:20:41.77 Elbow.reconfigure :tight @14:20:42.23 Elbow.seek :garage @14:20:45.18 Elbow.reconfigure :loose @14:20:45.28 Forearm.reconfigure :tight @14:20:45.33 Forearm.seek :garage @14:20:46.39 Hand.open @14:20:47.06 Forearm.reconfigure :loose @14:20:47.11 Forearm.seek :retract @14:20:48.16 delayUntil "14:24:54.61" @14:20:48.19 Whole Cell Archive sampling 100ml @14:20:48.20 Loading wcr puck from tube 7 into Collection Clamp @14:20:48.45 SC.reconfigure :tight @14:20:48.50 SC.seek 7 @14:20:48.62 SC.configure :limp @14:20:48.84 Elbow.reconfigure :tight @14:20:48.89 Elbow.seek 7 @14:20:50.82 Forearm.reconfigure :tight @14:20:50.88 Forearm.seek 7 + 450 counts @14:20:51.87 SE.reconfigure :pick @14:20:51.93 SE.seek :beamBreak @14:20:54.56 SE.configure :default @14:20:54.73 Elbow.seek 7 @14:20:54.92 Forearm.seek 7 + 300 counts @14:20:55.12 Forearm.reconfigure :loose @14:20:55.17 Hand.close @14:20:55.76 SE.seek :down @14:20:55.97 Forearm.seek :retract @14:20:56.93 Elbow.seek CC @14:20:58.03 SE.stop @14:20:58.53 Elbow.reconfigure :loose @14:20:58.64 Forearm.reconfigure :tight @14:20:58.69 Forearm.seek CC @14:20:59.97 CC.seek :closed @14:21:04.51 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.30 @14:21:04.52 Hand.open @14:21:05.18 Forearm.reconfigure :loose @14:21:05.24 Forearm.seek :retract @14:21:06.22 collectSample 100ml w/bubble point of 35psi @14:21:06.23 Priming 7ml @14:21:06.25 Intake.select :close @14:21:06.26 Exhaust.select :close @14:21:06.27 SS.hold @14:21:06.48 Calibrating pressure sensors @14:21:06.49 CTV.dial :air @14:21:06.57 SSV.dial :spit,avoiding: :exhaust @14:21:06.59 SS.reconfigure :fast @14:21:06.64 SS.seek 5ml @14:21:09.35 SSV.dial CBV @14:21:09.37 CBV.dial SSV @14:21:10.80 delay 3.8 seconds @14:21:14.74 Sensor limits: Intake max 98.4psia; Outlet max 92.8psia @14:21:14.75 SSV.dial :spit @14:21:16.11 SS.seek 0ml @14:21:19.10 SSV.dial CBV @14:21:20.46 CTV.dial :intake @14:21:21.24 SSV.dial CBV @14:21:21.26 CBV.dial SSV @14:21:21.41 Allowing 40s for 7.0ml of remaining 7.0ml @14:21:21.43 Intake.select :open @14:21:24.01 Intake @15.5psia, vacuum from 10.0 to 13.0psi @14:21:24.06 SS.reconfigure :inhaling 2.20ml/s @14:21:24.11 SS.seek 7ml @14:21:31.28 Stroke completed -- waiting for pressure to equalize ... @14:21:31.30 SS.reconfigure :exhale @14:21:31.34 SS.hold @14:21:49.78 Intake.select :close @14:21:49.94 SSV.dial :exhaust @14:21:50.69 Exhaust.select :open @14:21:50.81 SS.seek 0ml @14:21:54.97 Exhaust.select :close @14:21:55.48 Sampling 100.0ml @14:21:55.49 Intake.select :close @14:21:55.50 Exhaust.select :close @14:21:55.52 SS.reconfigure :default @14:21:55.56 SS.hold @14:21:55.78 CTV.dial :intake @14:21:55.86 SSV.dial CBV @14:21:55.89 CBV.dial SSV @14:21:56.68 Allowing 130s for 25.0ml of remaining 100.0ml @14:21:56.69 Intake.select :open @14:21:58.86 Intake @16.0psia, vacuum from 10.0 to 13.0psi @14:21:58.88 SS.reconfigure :inhaling 2.20ml/s @14:21:58.93 SS.seek 25ml @14:22:05.62 Vacuum too high -- waiting up to 40 seconds @14:22:05.64 SS.reconfigure :exhale @14:22:05.69 SS.hold @14:22:08.08 Intake @14.1psia, vacuum from 8.6 to 11.6psi @14:22:08.10 SS.reconfigure :inhaling 1.76ml/s @14:22:08.16 SS.seek 25ml @14:22:19.53 Stroke completed -- waiting for pressure to equalize ... @14:22:19.54 SS.reconfigure :exhale @14:22:19.59 SS.hold @14:22:28.19 Intake.select :close @14:22:28.35 SSV.dial :exhaust @14:22:29.13 Exhaust.select :open @14:22:29.25 SS.seek 0ml @14:22:38.17 Exhaust.select :close @14:22:38.28 SSV.dial CBV @14:22:38.30 CBV.dial SSV @14:22:39.11 Allowing 130s for 25.0ml of remaining 75.0ml @14:22:39.13 Intake.select :open @14:22:41.35 Intake @16.1psia, vacuum from 10.0 to 13.0psi @14:22:41.37 SS.reconfigure :inhaling 1.76ml/s @14:22:41.41 SS.seek 25ml @14:22:53.24 Vacuum too high -- waiting up to 40 seconds @14:22:53.26 SS.reconfigure :exhale @14:22:53.32 SS.hold @14:22:55.58 Intake @14.0psia, vacuum from 8.5 to 11.5psi @14:22:55.60 SS.reconfigure :inhaling 1.41ml/s @14:22:55.65 SS.seek 25ml @14:23:03.83 Stroke completed -- waiting for pressure to equalize ... @14:23:03.84 SS.reconfigure :exhale @14:23:03.89 SS.hold @14:23:08.95 Intake.select :close @14:23:09.12 SSV.dial :exhaust @14:23:09.88 Exhaust.select :open @14:23:09.99 SS.seek 0ml @14:23:18.94 Exhaust.select :close @14:23:19.05 SSV.dial CBV @14:23:19.09 CBV.dial SSV @14:23:19.86 Allowing 130s for 25.0ml of remaining 50.0ml @14:23:19.88 Intake.select :open @14:23:22.05 Intake @16.0psia, vacuum from 10.0 to 13.0psi @14:23:22.07 SS.reconfigure :inhaling 1.41ml/s @14:23:22.11 SS.seek 25ml @14:23:43.15 Stroke completed -- waiting for pressure to equalize ... @14:23:43.17 SS.reconfigure :exhale @14:23:43.22 SS.hold @14:23:50.08 Intake.select :close @14:23:50.24 SSV.dial :exhaust @14:23:51.03 Exhaust.select :open @14:23:51.15 SS.seek 0ml @14:24:00.31 Exhaust.select :close @14:24:00.42 SSV.dial CBV @14:24:00.46 CBV.dial SSV @14:24:01.24 Allowing 130s for 25.0ml of remaining 25.0ml @14:24:01.25 Intake.select :open @14:24:03.42 Intake @16.0psia, vacuum from 10.0 to 13.0psi @14:24:03.44 SS.reconfigure :inhaling 1.41ml/s @14:24:03.50 SS.seek 25ml @14:24:24.33 Stroke completed -- waiting for pressure to equalize ... @14:24:24.34 SS.reconfigure :exhale @14:24:24.39 SS.hold @14:24:30.90 Intake.select :close @14:24:31.06 SSV.dial :exhaust @14:24:31.83 Exhaust.select :open @14:24:31.95 SS.seek 0ml @14:24:40.95 Exhaust.select :close @14:24:41.07 Sampled 100.0ml @14:24:41.08 Drying puck before fixing for 5:00 @14:24:41.09 CBV.dial :waste2b @14:24:41.86 delay 1:00 @14:24:55.06 recoverPRVreagent08 @14:24:55.08 PTV.dial :mixing @14:24:55.85 PBV.dial :waste1 @14:24:56.22 delay 30 seconds @14:25:26.25 PBV.dial between PSV and waste1 @14:25:26.63 PSV.dial :air @14:25:27.10 PS.reconfigure :fast @14:25:27.15 PS.seek 2.5ml @14:25:41.88 CSV.dial :air @14:25:42.35 CS.reconfigure :fast @14:25:42.40 CS.seek 10ml @14:25:51.34 PSV.dial :mixloop @14:25:51.82 delay 2 seconds @14:25:53.83 PSV.dial :air @14:25:54.30 delay 2 seconds @14:25:56.32 PSV.dial :mixloop @14:25:56.80 delay 2 seconds @14:25:58.82 PSV.dial :air @14:25:59.29 PS.seek 0ml @14:26:07.34 Pumping wcr puck from tube 7 up to 35psi... @14:26:07.36 CBV.dial SSV @14:26:08.63 CBV.dial between CSV and waste2b @14:26:09.62 CSV.dial between air and CTV @14:26:09.96 CS.seek 7.358ml @14:26:16.81 CTV.dial CSV,avoiding: :air @14:26:16.86 CSV.dial CTV @14:26:17.61 CS.reconfigure :pressurize @14:26:17.66 CS.seek 0ml @14:26:23.29 washCycleDA #1 of 2 @14:26:23.30 PSR.select :air @14:26:23.32 PSV.dial PSR @14:26:24.67 Pulling 0.5ml of PSR.wash @14:26:24.68 PSR.select :wash @14:26:24.80 PS.reconfigure :default @14:26:24.85 PS.seek 0.5ml @14:26:30.61 CBV.dial :waste2b @14:26:30.81 delay 5 seconds @14:26:30.97 CS.reconfigure :default @14:26:31.02 CS.seek 0ml @14:26:35.83 PSR.select :air @14:26:35.95 PS.seek 2ml @14:26:38.40 delay 1:00 @14:26:52.09 PTV.dial :puck @14:26:52.84 PSV.dial PTV @14:26:53.59 PBV.dial :waste1 @14:26:54.03 PS.seek 1.5ml @14:26:59.94 delay 3 seconds @14:27:02.96 PBV.dial between PSV and waste1 @14:27:03.34 PS.seek 0ml @14:27:19.94 delay 2:00 @14:27:38.43 CTV.dial :air,avoiding: :intake @14:27:39.78 Fix #1 of 2 (with RNAlater) for 2:30 @14:27:39.80 CSV.dial CSR @14:27:40.36 Pulling 2ml of CSR.RNAlater @14:27:40.37 CSR.select :RNAlater @14:27:40.49 CS.seek 2ml @14:27:46.46 delay 5 seconds @14:27:51.49 CSR.select :air @14:27:51.61 CS.reconfigure :fast @14:27:51.66 CS.seek 10ml @14:28:11.72 Pumping wcr puck from tube 7 up to 35psi... @14:28:11.73 CBV.dial SSV @14:28:13.00 CBV.dial between CSV and waste2b @14:28:13.98 CSV.dial between air and CTV @14:28:14.58 CS.seek 7.308ml @14:28:21.62 CTV.dial CSV,avoiding: :air @14:28:21.65 CSV.dial CTV @14:28:23.05 CS.reconfigure :pressurize @14:28:23.10 CS.seek 0ml @14:28:41.55 delay 2:30 @14:29:19.97 recoverPSRreagent08 @14:29:19.98 PTV.dial :puck @14:29:20.06 PBV.dial :waste1 @14:29:20.44 delay 30 seconds @14:29:50.46 PBV.dial between PSV and waste1 @14:29:50.84 PSR.select :air @14:29:50.86 PSV.dial PSR @14:29:51.63 PS.reconfigure :fast @14:29:51.68 PS.seek 2.5ml @14:30:15.93 PSV.dial PTV @14:30:16.83 delay 5 seconds @14:30:21.85 PSV.dial PSR @14:30:22.59 delay 5 seconds @14:30:27.61 PSV.dial PTV @14:30:28.39 delay 5 seconds @14:30:33.41 PSV.dial :air @14:30:34.17 PS.seek 0ml @14:30:57.88 washCycleDA #2 of 2 @14:30:57.89 PSR.select :air @14:30:57.91 PSV.dial PSR @14:30:59.25 Pulling 0.5ml of PSR.wash @14:30:59.26 PSR.select :wash @14:30:59.38 PS.reconfigure :default @14:30:59.43 PS.seek 0.5ml @14:31:05.43 delay 5 seconds @14:31:10.45 PSR.select :air @14:31:10.57 PS.seek 2ml @14:31:11.57 CBV.dial :waste2b @14:31:11.92 CS.reconfigure :default @14:31:11.97 CS.seek 0ml @14:31:13.63 delay 1:00 @14:31:26.78 PTV.dial :puck @14:31:26.85 PSV.dial PTV @14:31:27.60 PBV.dial :waste1 @14:31:28.04 PS.seek 1.5ml @14:31:33.96 delay 3 seconds @14:31:36.98 PBV.dial between PSV and waste1 @14:31:37.36 PS.seek 0ml @14:31:53.53 delay 2:00 @14:32:13.65 CTV.dial :air,avoiding: :intake @14:32:15.00 Fix #2 of 2 (with RNAlater) for 2:30 @14:32:15.01 CSV.dial CSR @14:32:15.44 Pulling 2ml of CSR.RNAlater @14:32:15.45 CSR.select :RNAlater @14:32:15.58 CS.seek 2ml @14:32:21.55 delay 5 seconds @14:32:26.57 CSR.select :air @14:32:26.69 CS.reconfigure :fast @14:32:26.75 CS.seek 10ml @14:32:46.84 Pumping wcr puck from tube 7 up to 35psi... @14:32:46.85 CBV.dial SSV @14:32:48.12 CBV.dial between CSV and waste2b @14:32:49.11 CSV.dial between air and CTV @14:32:49.84 CS.seek 7.279ml @14:32:56.99 CTV.dial CSV,avoiding: :air @14:32:57.02 CSV.dial CTV @14:32:58.43 CS.reconfigure :pressurize @14:32:58.49 CS.seek 0ml @14:33:12.70 delay 2:30 @14:33:53.55 recoverPSRreagent08 @14:33:53.56 PTV.dial :puck @14:33:53.63 PBV.dial :waste1 @14:33:54.02 delay 30 seconds @14:34:24.05 PBV.dial between PSV and waste1 @14:34:24.43 PSR.select :air @14:34:24.45 PSV.dial PSR @14:34:25.22 PS.reconfigure :fast @14:34:25.27 PS.seek 2.5ml @14:34:49.49 PSV.dial PTV @14:34:50.26 delay 5 seconds @14:34:55.28 PSV.dial PSR @14:34:56.04 delay 5 seconds @14:35:01.06 PSV.dial PTV @14:35:01.94 delay 5 seconds @14:35:06.96 PSV.dial :air @14:35:07.75 PS.seek 0ml @14:35:31.34 getPMLReagent08 @14:35:31.35 PRV.dial :air @14:35:31.82 delay 5 seconds @14:35:37.27 PTV.dial :PRVmixing @14:35:38.65 delay 5 seconds @14:35:42.72 CBV.dial :waste2b @14:35:43.07 CS.reconfigure :default @14:35:43.12 CS.seek 0ml @14:35:43.66 PRV.dial :ab2 @14:35:44.13 PTV.dial :PRVmixing @14:35:44.32 PSV.dial :mixloop @14:35:44.81 PS.reconfigure :default @14:35:44.86 PS.seek 0.3ml @14:35:48.75 delay 1:00 @14:35:48.78 delay 2 seconds @14:35:50.79 PRV.dial :air @14:35:51.33 PS.seek 0.55ml @14:35:54.68 addPMLReagent08 @14:35:54.69 PSV.dial :air @14:35:55.17 PS.seek 2ml @14:36:10.83 PSV.dial :PML @14:36:11.32 PTV.dial :mixing @14:36:12.10 PBV.dial :waste1 @14:36:12.53 PS.seek 1.85ml @14:36:14.86 PBV.dial between PSV and waste1 @14:36:15.25 PS.seek 0ml @14:36:35.18 delay 2 seconds @14:36:37.20 delay 8:00 @14:36:48.78 CTV.dial :air,avoiding: :intake @14:44:37.22 recoverPRVreagent08 @14:44:37.23 PTV.dial :mixing @14:44:37.31 PBV.dial :waste1 @14:44:37.68 delay 30 seconds @14:45:07.71 PBV.dial between PSV and waste1 @14:45:08.09 PSV.dial :air @14:45:08.70 PS.reconfigure :fast @14:45:08.75 PS.seek 2.5ml @14:45:33.07 PSV.dial :mixloop @14:45:33.53 delay 2 seconds @14:45:35.54 PSV.dial :air @14:45:36.01 delay 2 seconds @14:45:38.03 PSV.dial :mixloop @14:45:38.50 delay 2 seconds @14:45:40.52 PSV.dial :air @14:45:40.98 PS.seek 0ml @14:46:04.70 washCycleDA #1 of 3 @14:46:04.71 PSR.select :air @14:46:04.72 PSV.dial PSR @14:46:06.07 Pulling 0.5ml of PSR.wash @14:46:06.08 PSR.select :wash @14:46:06.20 PS.reconfigure :default @14:46:06.25 PS.seek 0.5ml @14:46:12.16 delay 5 seconds @14:46:17.18 PSR.select :air @14:46:17.30 PS.seek 2ml @14:46:33.44 PTV.dial :puck @14:46:34.20 PSV.dial PTV @14:46:34.95 PBV.dial :waste1 @14:46:35.38 PS.seek 1.5ml @14:46:41.29 delay 3 seconds @14:46:44.74 PBV.dial between PSV and waste1 @14:46:45.16 PS.seek 0ml @14:47:01.29 delay 2:00 @14:49:01.32 recoverPSRreagent08 @14:49:01.33 PTV.dial :puck @14:49:01.41 PBV.dial :waste1 @14:49:01.79 delay 30 seconds @14:49:31.81 PBV.dial between PSV and waste1 @14:49:32.19 PSR.select :air @14:49:32.21 PSV.dial PSR @14:49:32.98 PS.reconfigure :fast @14:49:33.03 PS.seek 2.5ml @14:49:57.28 PSV.dial PTV @14:49:58.18 delay 5 seconds @14:50:03.20 PSV.dial PSR @14:50:03.94 delay 5 seconds @14:50:08.96 PSV.dial PTV @14:50:09.72 delay 5 seconds @14:50:14.74 PSV.dial :air @14:50:15.53 PS.seek 0ml @14:50:39.26 washCycleDA #2 of 3 @14:50:39.27 PSR.select :air @14:50:39.28 PSV.dial PSR @14:50:40.63 Pulling 0.5ml of PSR.wash @14:50:40.64 PSR.select :wash @14:50:40.76 PS.reconfigure :default @14:50:40.81 PS.seek 0.5ml @14:50:46.72 delay 5 seconds @14:50:51.74 PSR.select :air @14:50:51.86 PS.seek 2ml @14:51:08.03 PTV.dial :puck @14:51:08.11 PSV.dial PTV @14:51:08.86 PBV.dial :waste1 @14:51:09.30 PS.seek 1.5ml @14:51:15.28 delay 3 seconds @14:51:18.30 PBV.dial between PSV and waste1 @14:51:18.68 PS.seek 0ml @14:51:34.78 delay 2:00 @14:53:34.81 recoverPSRreagent08 @14:53:34.82 PTV.dial :puck @14:53:34.89 PBV.dial :waste1 @14:53:35.28 delay 30 seconds @14:54:05.31 PBV.dial between PSV and waste1 @14:54:05.69 PSR.select :air @14:54:05.71 PSV.dial PSR @14:54:06.47 PS.reconfigure :fast @14:54:06.52 PS.seek 2.5ml @14:54:30.68 PSV.dial PTV @14:54:31.45 delay 5 seconds @14:54:36.47 PSV.dial PSR @14:54:37.25 delay 5 seconds @14:54:42.27 PSV.dial PTV @14:54:43.16 delay 5 seconds @14:54:48.18 PSV.dial :air @14:54:48.96 PS.seek 0ml @14:55:12.50 washCycleDA #3 of 3 @14:55:12.51 PSR.select :air @14:55:12.53 PSV.dial PSR @14:55:13.87 Pulling 0.5ml of PSR.wash @14:55:13.88 PSR.select :wash @14:55:14.00 PS.reconfigure :default @14:55:14.05 PS.seek 0.5ml @14:55:19.97 delay 5 seconds @14:55:24.99 PSR.select :air @14:55:25.52 PS.seek 2ml @14:55:41.71 PTV.dial :puck @14:55:41.79 PSV.dial PTV @14:55:42.67 PBV.dial :waste1 @14:55:43.10 PS.seek 1.5ml @14:55:49.09 delay 3 seconds @14:55:52.11 PBV.dial between PSV and waste1 @14:55:52.49 PS.seek 0ml @14:56:08.84 delay 2:00 @14:58:08.87 recoverPSRreagent08 @14:58:08.88 PTV.dial :puck @14:58:08.96 PBV.dial :waste1 @14:58:09.34 delay 30 seconds @14:58:39.37 PBV.dial between PSV and waste1 @14:58:39.74 PSR.select :air @14:58:39.76 PSV.dial PSR @14:58:40.64 PS.reconfigure :fast @14:58:40.69 PS.seek 2.5ml @14:59:04.96 PSV.dial PTV @14:59:05.84 delay 5 seconds @14:59:10.86 PSV.dial PSR @14:59:11.60 delay 5 seconds @14:59:16.62 PSV.dial PTV @14:59:17.53 delay 5 seconds @14:59:22.55 PSV.dial :air @14:59:23.34 PS.seek 0ml @14:59:46.99 Gate.power :camera,:ON @14:59:47.01 Mixing DA Substrates @14:59:47.02 PSR.select :air @14:59:47.03 PSV.dial PSR @14:59:48.47 Pulling 0.25ml of PSR.sub1 @14:59:48.51 PSR.select :sub1 @14:59:48.71 PS.reconfigure :default @14:59:48.82 PS.seek 0.25ml @14:59:52.22 delay 5 seconds @14:59:57.24 Pulling 0.25ml of PSR.sub2 @14:59:57.26 PSR.select :sub2 @14:59:57.37 PS.seek 0.5ml @15:00:00.80 delay 5 seconds @15:00:05.82 PSR.select :air @15:00:05.94 PS.seek 2ml @15:00:22.15 PTV.dial :puck @15:00:22.23 PSV.dial PTV @15:00:23.02 PBV.dial :waste1 @15:00:23.47 PS.seek 1.5ml @15:00:29.37 delay 3 seconds @15:00:32.39 PBV.dial between PSV and waste1 @15:00:32.78 PS.seek 0ml @15:00:49.08 delay 10 seconds @15:00:59.11 recoverPSRreagent08 @15:00:59.12 PTV.dial :puck @15:00:59.19 PBV.dial :waste1 @15:00:59.59 delay 30 seconds @15:01:29.61 PBV.dial between PSV and waste1 @15:01:30.01 PSR.select :air @15:01:30.03 PSV.dial PSR @15:01:30.84 PS.reconfigure :fast @15:01:30.89 PS.seek 2.5ml @15:01:56.02 PSV.dial PTV @15:01:56.83 delay 5 seconds @15:02:01.85 PSV.dial PSR @15:02:02.76 delay 5 seconds @15:02:07.78 PSV.dial PTV @15:02:09.04 delay 5 seconds @15:02:14.06 PSV.dial :air @15:02:14.87 PS.seek 0ml @15:02:39.80 Additional air clear @15:02:39.81 PSR.select :air @15:02:39.83 PSV.dial PSR @15:02:41.22 Pulling 2ml of PSR.air @15:02:41.23 PSR.select :air @15:02:41.26 PS.reconfigure :default @15:02:41.30 PS.seek 2ml @15:03:02.61 delay 5 seconds @15:03:07.64 PTV.dial :puck @15:03:07.72 PSV.dial PTV @15:03:08.63 PBV.dial :waste1 @15:03:09.06 PS.seek 1.5ml @15:03:15.02 delay 3 seconds @15:03:18.04 PBV.dial between PSV and waste1 @15:03:18.43 delay 1 second @15:03:19.45 PSV.dial :waste1 @15:03:20.55 PS.seek 0ml @15:03:36.73 Processing Puck Heater at 28C before imaging @15:03:36.74 PH.coast @15:03:36.86 Imaging da puck in Processing Clamp while storing it to tube 6 @15:03:37.03 SC.reconfigure :tight @15:03:37.08 SC.seek 6 @15:03:40.64 SC.configure :limp @15:03:40.86 Elbow.reconfigure :tight @15:03:40.91 Elbow.seek 6 @15:03:42.71 Forearm.reconfigure :tight @15:03:42.77 Forearm.seek 6 + 450 counts @15:03:43.88 SE.reconfigure :pick @15:03:43.94 SE.seek :beamBreak @15:03:45.85 SE.configure :default @15:03:46.01 Elbow.reconfigure :limp @15:03:46.06 Forearm.reconfigure :loose @15:03:46.11 Forearm.seek 6 @15:03:46.38 Elbow.hold @15:03:46.45 Elbow.reconfigure :tight @15:03:46.55 SE.seek 20.1 Pucks below Top Plate @15:03:47.04 Forearm.force -20 @15:03:47.08 Forearm.seek 6 + 299 counts @15:03:47.79 Forearm.reconfigure :tight @15:03:47.91 Forearm.seek 6 + 450 counts @15:03:48.11 SE.seek 21.1 Pucks below Top Plate @15:03:48.70 Forearm.reconfigure :loose @15:03:48.75 Forearm.seek :retract @15:03:49.73 Elbow.seek PC @15:03:52.41 Elbow.reconfigure :loose @15:03:52.51 Forearm.seek PC @15:03:53.48 Hand.close @15:03:54.21 PC.seek :open @15:03:59.01 Forearm.seek :retract @15:03:59.98 Elbow.reconfigure :tight @15:04:00.03 Elbow.seek 6 @15:04:02.68 Forearm.reconfigure :tight @15:04:02.73 Forearm.seek 6 + 450 counts @15:04:03.84 Hand.open @15:04:04.65 SE.seek 20.75 Pucks below Top Plate @15:04:04.97 SE.reconfigure :seal @15:04:05.02 SE.seek 19.79 Pucks below Top Plate @15:04:05.52 Lights, camera, ACTION! @15:04:05.55 PuckCamera: snap -bin=2,2 -auto /var/log/esp/da23dec2215h100ml.tif @15:04:25.08 Waiting >20 seconds for da to relinquish FlushPuck @15:04:28.92 SX HX9/16 (USB:02): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 19.881 seconds /var/log/esp/da23dec2215h100ml.tif: TIFF Upload Complete @15:04:28.95 PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da23dec2215h100ml15s.tif @15:04:44.56 SX HX9/16 (USB:02): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15 seconds /var/log/esp/midres/da23dec2215h100ml15s.tif: TIFF Upload Complete @15:04:44.59 PuckCamera: snap -bin=2,2 20.0 /var/log/esp/midres/da23dec2215h100ml20s.tif @15:05:05.24 SX HX9/16 (USB:02): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 20 seconds /var/log/esp/midres/da23dec2215h100ml20s.tif: TIFF Upload Complete @15:05:05.26 Gate.power :camera,:OFF @15:05:05.28 DAfans.email "19.881 sec auto-exposure",Subject: "da23dec2215h100ml images!" @15:05:05.70 Elbow.seek 6 @15:05:05.94 Forearm.seek 6 + 450 counts @15:05:06.11 SE.reconfigure :gently @15:05:06.18 SE.seek 20.5 Pucks below Top Plate @15:05:06.91 Elbow.reconfigure :limp @15:05:06.97 Forearm.reconfigure :loose @15:05:07.03 Forearm.seek 6 @15:05:07.65 Elbow.hold @15:05:07.73 Elbow.reconfigure :tight @15:05:07.78 SE.reconfigure :default @15:05:07.85 SE.seek 20.69 Pucks below Top Plate @15:05:08.31 Forearm.force -20 @15:05:08.35 Forearm.seek 6 + 318 counts @15:05:08.68 Forearm.reconfigure :tight @15:05:08.73 Forearm.seek 6 + 450 counts @15:05:09.12 SE.seek :down @15:05:10.29 Forearm.reconfigure :loose @15:05:10.35 Forearm.seek :retract @15:05:10.43 SE.stop @15:05:11.48 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @15:05:12.06 Elbow.seek FlushPuck::Garage @15:05:14.00 Elbow.reconfigure :loose @15:05:14.21 Forearm.seek :garage @15:05:15.32 Hand.close @15:05:16.24 Forearm.seek :retract @15:05:17.32 Elbow.reconfigure :tight @15:05:17.42 Elbow.seek PC @15:05:18.83 Elbow.reconfigure :loose @15:05:19.04 Forearm.reconfigure :tight @15:05:19.14 Forearm.seek PC @15:05:20.82 PC.seek :closed @15:05:25.74 Clamp::VelocityError in psFlush -- Processing Clamp did not close properly @15:05:25.76 Retry #1 of 2 @15:05:26.20 PC.seek :open @15:05:31.14 PC.seek :closed @15:05:31.47 Waiting >20 seconds for psFlush to relinquish Arm @15:05:35.95 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.68 @15:05:36.09 Hand.open @15:05:36.86 Forearm.reconfigure :loose @15:05:36.96 Forearm.seek :retract @15:05:37.95 PSR.select :air @15:05:38.01 Storing wcr puck in Collection Clamp to tube 6 @15:05:38.05 PSV.dial PSR @15:05:38.59 SC.reconfigure :tight @15:05:38.68 SC.seek 6 @15:05:38.84 Pulling 0.5ml of PSR.flush @15:05:38.88 PSR.select :flush @15:05:39.02 SC.configure :limp @15:05:39.34 PS.seek 0.5ml @15:05:39.68 Elbow.reconfigure :tight @15:05:39.80 Elbow.seek 6 @15:05:42.41 upsync @15:05:42.45 Forearm.reconfigure :tight @15:05:42.51 Forearm.seek 6 + 450 counts @15:05:43.58 SE.reconfigure :pick @15:05:43.65 SE.seek :beamBreak @15:05:45.27 delay 5 seconds @15:05:45.36 SE.configure :default @15:05:45.99 Elbow.reconfigure :limp @15:05:46.04 Forearm.reconfigure :loose @15:05:46.09 Forearm.seek 6 @15:05:46.38 Elbow.hold @15:05:46.45 Elbow.reconfigure :tight @15:05:46.56 SE.seek 21.1 Pucks below Top Plate @15:05:47.09 Forearm.force -20 @15:05:47.13 Forearm.seek 6 + 287 counts @15:05:47.45 Forearm.reconfigure :tight @15:05:47.51 Forearm.seek 6 + 450 counts @15:05:47.70 SE.seek 22.1 Pucks below Top Plate @15:05:48.27 Forearm.reconfigure :loose @15:05:48.32 Forearm.seek :retract @15:05:49.28 Elbow.seek CC @15:05:50.29 PSR.select :air @15:05:50.41 PS.seek 2.5ml @15:05:50.91 Elbow.reconfigure :loose @15:05:51.01 Forearm.seek CC @15:05:51.96 Hand.close @15:05:52.60 CC.seek :open @15:05:57.38 Forearm.seek :retract @15:05:58.37 Elbow.reconfigure :tight @15:05:58.42 Elbow.seek 6 @15:06:00.21 Elbow.reconfigure :limp @15:06:00.26 Forearm.seek 6 @15:06:01.29 Elbow.hold @15:06:01.36 Elbow.reconfigure :tight @15:06:01.85 Hand.open @15:06:02.59 Forearm.force -20 @15:06:02.64 Forearm.seek 6 + 314 counts @15:06:02.95 Forearm.reconfigure :tight @15:06:03.00 Forearm.seek 6 + 450 counts @15:06:03.28 SE.seek :down @15:06:04.41 Forearm.reconfigure :loose @15:06:04.46 Forearm.seek :retract @15:06:04.53 SE.stop @15:06:05.39
Starting finalPurge @15:06:11.70 delay 1 second @15:06:12.72 PRV.dial :waste2,avoiding: :ab2 @15:06:14.61 PTV.dial :PRVmixing @15:06:15.94 PSV.dial :mixloop @15:06:17.10 PS.seek :empty @15:06:24.58
Waiting >20 seconds for psFlush to relinquish FlushPuck @15:06:43.70 PSR.select :air @15:06:43.72 PSV.dial PSR @15:06:44.77 Pulling 0.5ml of PSR.flush @15:06:44.78 PSR.select :flush @15:06:44.90 PS.seek 0.5ml @15:06:50.80 delay 5 seconds @15:06:55.82 PSR.select :air @15:06:55.94 PS.seek 2.5ml @15:07:17.26 delay 1 second @15:07:18.28 PBV.dial :waste1 @15:07:18.65 PTV.dial :mixing @15:07:19.42 PSV.dial :mixloop @15:07:20.49 PS.seek :empty @15:07:46.89 PSV.dial PSR @15:07:47.93 PSR.select :flush @15:07:48.05 PS.seek 1ml @15:07:59.14 PSR.select :air @15:07:59.26 PS.seek 2.5ml @15:08:15.42 PSV.dial PTV @15:08:16.18 PTV.dial :puck @15:08:16.92 PBV.dial :waste1 @15:08:17.00 PS.seek 0ml @15:08:43.42 PSV.dial PSR @15:08:44.18 PSR.select :flush @15:08:44.69 PS.seek 0.5ml @15:08:50.57 PSR.select :air @15:08:50.70 PS.seek 2.5ml @15:09:11.95 PSV.dial PTV @15:09:12.71 PBV.dial PSV @15:09:13.29 PS.seek 0ml @15:09:39.82 PSV.dial PBV @15:09:40.30 PS.seek 2.5ml @15:10:06.73 PSV.dial :waste1 @15:10:07.49 PS.seek 0ml @15:10:33.79 PSV.dial PSR @15:10:34.26 PSR.select :air @15:10:34.29 PS.seek 2.5ml @15:11:00.84 PSV.dial PTV @15:11:01.63 PBV.dial PSV @15:11:01.75 PS.seek 0ml @15:11:28.37 PSV.dial PBV @15:11:28.86 PS.seek 2.5ml @15:11:55.30 PSV.dial :waste1 @15:11:56.06 PS.seek 0ml @15:12:22.41 PSV.dial PSR @15:12:22.88 PSR.select :flush @15:12:23.01 PS.seek 1ml @15:12:34.10 PSR.select :air @15:12:34.22 PS.seek 2.5ml @15:12:50.35 PSV.dial PTV @15:12:51.08 PTV.dial :puck @15:12:51.16 PBV.dial :waste1 @15:12:51.73 PS.seek 0ml @15:13:18.04 PSV.dial PSR @15:13:18.80 PSR.select :flush @15:13:18.92 PS.seek 0.5ml @15:13:25.19 PSR.select :air @15:13:25.31 PS.seek 2.5ml @15:13:46.75 PSV.dial PTV @15:13:47.52 PBV.dial PSV @15:13:48.09 PS.seek 0ml @15:14:14.44 PSV.dial PBV @15:14:14.92 PS.seek 2.5ml @15:14:41.35 PSV.dial :waste1 @15:14:42.09 PS.seek 0ml @15:15:08.44 PSV.dial PSR @15:15:08.91 PSR.select :air @15:15:09.38 PS.seek 2.5ml @15:15:35.72 PSV.dial PTV @15:15:36.46 PBV.dial PSV @15:15:36.65 PS.seek 0ml @15:16:03.19 PSV.dial PBV @15:16:03.67 PS.seek 2.5ml @15:16:29.97 PSV.dial :waste1 @15:16:30.73 PS.seek 0ml @15:16:57.10
Loading FlushPuck into Collection Clamp from Processing Clamp @15:16:57.29 Elbow.seek PC @15:16:59.79 Elbow.reconfigure :loose @15:16:59.89 Forearm.seek PC @15:17:00.87 Hand.close @15:17:01.45 PC.seek :open @15:17:06.18 Forearm.seek :retract @15:17:07.24 Elbow.reconfigure :tight @15:17:07.36 Elbow.seek CC @15:17:10.99 Elbow.reconfigure :loose @15:17:11.10 Forearm.reconfigure :tight @15:17:11.14 Forearm.seek CC @15:17:12.41 CC.seek :closed @15:17:16.95 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.43 @15:17:17.02 Hand.open @15:17:17.64 Forearm.reconfigure :loose @15:17:17.69 Forearm.seek :retract @15:17:18.70 Flushing the Collection Valve::Manifold @15:17:18.83 CSV.dial CSR @15:17:19.73 Pulling 5ml of CSR.flush CSR.select :flush @15:17:19.85 CS.seek 5ml @15:17:33.41 delay 5 seconds @15:17:38.43 CSR.select :air @15:17:38.60 CS.reconfigure :fast @15:17:38.65 CS.seek 7.499ml @15:17:45.47 CSV.dial :waste @15:17:46.79 CS.reconfigure :default @15:17:46.84 CS.seek 0ml @15:18:07.01 Flushing lower manifold's waste1 port @15:18:07.14 CSV.dial :flush @15:18:07.59 CS.reconfigure :fast @15:18:07.64 CS.seek 5ml @15:18:20.72 delay 5 seconds @15:18:25.74 CSV.dial :air @15:18:26.25 CS.seek 7ml @15:18:31.82 CSV.dial CTV @15:18:31.84
CTV.dial CSV @15:18:33.27 CBV.dial :waste1 @15:18:34.04 CS.seek 0ml @15:18:51.01 Flushing lower manifold's waste2 port @15:18:51.13 CSV.dial :flush @15:18:51.87 CS.seek 5ml @15:19:04.66 delay 5 seconds @15:19:09.68 CSV.dial :air @15:19:10.22 CS.seek 7ml @15:19:15.76 CSV.dial CTV @15:19:15.78
CTV.dial CSV @15:19:16.27 CBV.dial :waste2 @15:19:17.32 CS.seek 0ml @15:19:34.29 Flushing lower manifold's waste2b port @15:19:34.41 CSV.dial :flush @15:19:35.17 CS.seek 5ml @15:19:48.19 delay 5 seconds @15:19:53.21 CSV.dial :air @15:19:53.86 CS.seek 7ml @15:19:59.41 CSV.dial CTV @15:19:59.44
CTV.dial CSV @15:19:59.89 CBV.dial :waste2b @15:20:00.96 CS.seek 0ml @15:20:17.91 Disinfecting external Intake with kill @15:20:18.03 CSV.dial CSR @15:20:18.49 Pulling 7.999ml of CSR.kill @15:20:18.50 CSR.select :kill @15:20:18.61 CS.seek 8ml @15:20:39.35 delay 5 seconds @15:20:44.37 CSR.select :air @15:20:44.49 CS.seek 10ml @15:20:50.16 CSV.dial CBV @15:20:50.18
CBV.dial CSV @15:20:51.25 CTV.dial :intake @15:20:52.02 Intake.select :open @15:20:52.13 CS.seek 2ml @15:21:11.25 Intake.select :close @15:21:11.36 CTV.dial :air @15:21:12.13 CS.reconfigure :default @15:21:12.18 CS.seek 0ml @15:21:18.28 CSV.dial CSR @15:21:19.31 Pulling 5ml of CSR.flush @15:21:19.32 CSR.select :flush @15:21:19.44 CS.seek 5ml @15:21:33.25 delay 5 seconds @15:21:38.27 CSR.select :air @15:21:38.39 CS.seek 10ml @15:21:52.06 CSV.dial CBV @15:21:53.10 CS.seek 0ml @15:22:19.56 Garaging FlushPuck in Collection Clamp @15:22:19.64 Elbow.reconfigure :tight @15:22:19.69 Elbow.seek CC @15:22:19.93 Elbow.reconfigure :loose @15:22:20.03 Forearm.seek CC @15:22:20.96 Hand.close @15:22:21.58 CC.seek :open @15:22:26.24 Forearm.seek :retract @15:22:27.23 Elbow.reconfigure :tight @15:22:27.28 Elbow.seek :garage @15:22:30.25 Elbow.reconfigure :loose @15:22:30.35 Forearm.reconfigure :tight @15:22:30.40 Forearm.seek :garage @15:22:31.48 Hand.open @15:22:32.13 Forearm.reconfigure :loose @15:22:32.18 Forearm.seek :retract @15:22:33.18 Completed [initialPurge; da [100, 100] #da and wsr are run simultanelously] begun 13:29:35 @15:22:33.24 Asleep after completing DA_WCR_122223 assays @15:22:33.26 GoodNews.email "{1 => 33,\n 2 => 33,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 23,\n 7 => 8}\nCTD data unavailable\nCan@15:18:54, 24% flow, 21.9C, 37% humidity, 15.4psia, 13.447V, 0.490A, 1.201Ah, 6.59W\nSampled 100.0ml",Subject: "Asleep after completing DA_WCR_122223 assays" @15:22:37.96 upsync + ESPwhich standAloneWCR_010224.rb + fn=standAloneWCR_010224.rb + IFS=: + set /home/esp/esp2/mission . + fullPath=/home/esp/esp2/mission/standAloneWCR_010224.rb + [ -f /home/esp/esp2/mission/standAloneWCR_010224.rb ] + realpath /home/esp/esp2/mission/standAloneWCR_010224.rb + return 0 + scriptPath=/home/esp/esp2local/mission/standAloneWCR_010224.rb + exec ruby /home/esp/esp2/lib/esp.rb /home/esp/esp2local/mission/standAloneWCR_010224.rb @18:24:10.59UTC02-Jan-24
ESP2 v3.42 I2C Gateway v3.20 for core bus @18:24:13.46 Configuration for short puck jake 1/11/21 by brent@mbari.org /home/esp/esp2/type/shallow/jake/configure.rb @18:24:17.40 Polling Can every 10:00 @18:24:17.42 Cleanly exited at 18:23:49UTC02-Jan-24 @18:24:17.46 Loading /home/esp/esp2local/mission/standAloneWCR_010224.rb mission @18:24:17.49 RuntimeError in MAIN -- Mission's startTube should be 3, not 2 @18:24:17.51 Waiting to Resume from Checkpoint "Loading /home/esp/esp2local/mission/standAloneWCR_010224.rb mission" @18:24:17.53 BadNews.email "Mission's startTube should be 3, not 2",Subject: "RuntimeError in MAIN" @18:24:22.02 upsync + ESPwhich standAloneWCR_010224.rb + fn=standAloneWCR_010224.rb + IFS=: + set /home/esp/esp2/mission . + fullPath=/home/esp/esp2/mission/standAloneWCR_010224.rb + [ -f /home/esp/esp2/mission/standAloneWCR_010224.rb ] + realpath /home/esp/esp2/mission/standAloneWCR_010224.rb + return 0 + scriptPath=/home/esp/esp2local/mission/standAloneWCR_010224.rb + exec ruby /home/esp/esp2/lib/esp.rb /home/esp/esp2local/mission/standAloneWCR_010224.rb Log::Locked in trapHandler -- Another process is already writing to /var/log/esp/real.log @18:26:36.49 Session Begun @18:26:44.95 -> abort!' mission complete' @18:26:44.97 CmdServer::Client::Error in dave -- Aborting Mission: mission complete @18:26:45.02 BadNews.email " mission complete",Subject: "standAloneWCR_010224 ABORTED!" # @18:26:49.37 upsync @18:26:49.45 Session Ended + ESPwhich standAloneWCR_010224.rb + fn=standAloneWCR_010224.rb + IFS=: + set /home/esp/esp2/mission . + fullPath=/home/esp/esp2/mission/standAloneWCR_010224.rb + [ -f /home/esp/esp2/mission/standAloneWCR_010224.rb ] + realpath /home/esp/esp2/mission/standAloneWCR_010224.rb + return 0 + scriptPath=/home/esp/esp2local/mission/standAloneWCR_010224.rb + exec ruby /home/esp/esp2/lib/esp.rb /home/esp/esp2local/mission/standAloneWCR_010224.rb @18:27:15.81UTC02-Jan-24
ESP2 v3.42 I2C Gateway v3.20 for core bus @18:27:18.57 Configuration for short puck jake 1/11/21 by brent@mbari.org /home/esp/esp2/type/shallow/jake/configure.rb @18:27:22.33 Polling Can every 10:00 @18:27:22.35 Cleanly exited at 18:26:49UTC02-Jan-24 @18:27:22.40 Loading /home/esp/esp2local/mission/standAloneWCR_010224.rb mission @18:27:22.46 Preparing to sleep until + 00:00:05 @18:27:22.49 GoodNews.email "Starting standAloneWCR_010224 w/pucks in tube 2\n{2 => 1}\nCTD data unavailable\nCan@18:27:22, 25% flow, 22.0C, 14% humidity, 14.9psia, 13.861V, 0.254A, 42.509Ah, 3.52W",Subject: "standAloneWCR_010224 begun" @18:27:22.52
Asleep til + 00:00:05 @18:27:22.56 Continuing at 18:27 @18:27:22.80 Gate.power :core,:OFF @18:27:22.81 Gate.power :raw,:OFF @18:27:22.83 Shutdown @18:27:25.85 GoodNews.email "Then, in tube 2: initialPurge; standAloneWCR([nil,100], $da)\n/home/esp/esp2local/mission/standAloneWCR_010224.rb:10\n{2 => 1}\nCTD data unavailable\nCan@18:27:22, 25% flow, 21.8C, 14% humidity, 14.9psia, 13.861V, 0.248A, 42.509Ah, 3.44W",Subject: "Asleep until 18:27" @18:27:27.19 upsync @18:27:27.43
Powering core bus @18:27:30.29 upsync @18:27:32.97
Gate.power :raw,:ON @18:27:33.61 CS.configure :default @18:27:33.65 PS.configure :default @18:27:33.70 SS.configure :default @18:27:33.75 CC.configure :default @18:27:33.80 PC.configure :default @18:27:33.86 Forearm.configure :loose @18:27:33.91 Elbow.configure :loose @18:27:33.98 SC.configure :loose @18:27:34.03 SE.configure :default @18:27:34.08 CH.configure :default @18:27:34.11 PH.configure :default @18:27:34.15 All dwarf controllers running firmware version 3.85 @18:27:34.26 Valve.reset @18:27:34.52 Intake.select :close @18:27:34.56 Exhaust.select :close @18:27:34.57 CTV.dial :air @18:27:34.68 PSV.dial :air @18:27:34.72 CSV.dial :waste1 @18:27:34.83
Forearm.home.seek :retract @18:27:35.20 SSV.dial :spit,avoiding: :exhaust @18:27:35.51 CS.reconfigure :fast @18:27:35.54 SS.home.seek 0ml @18:27:35.67 CS.home.seek 0ml @18:27:35.70 Hand.close @18:27:35.73
Elbow.reconfigure :loose @18:27:35.84 Elbow.jog 1500 @18:27:35.91 PS.home.seek 0ml @18:27:36.33
Elbow.reconfigure :loose @18:27:36.40 Elbow.home.seek :clear @18:27:39.65 SE.home.seek :down @18:27:40.27 SC.reconfigure :tight @18:27:40.32 SC.home.seek :initial @18:27:44.23 PS.stop @18:28:05.56
SC.configure :limp @18:28:05.61 Forearm.seek :retract @18:28:05.68 Elbow.seek :hammer @18:28:06.76 Forearm.reconfigure :tight @18:28:06.81 Forearm.seek retracted @18:28:07.05 PC.home.seek :open @18:28:07.08
CC.home.seek :open @18:28:10.44 Phase [initialPurge; standAloneWCR([nil,100], $da)] @18:28:10.46 Notice.email "At 18:28:10 began in tube 2: initialPurge; standAloneWCR([nil,100], $da)\n/home/esp/esp2local/mission/standAloneWCR_010224.rb:10\n{2 => 1}\nCTD data unavailable\nCan@18:28:10, 25% flow, 22.0C, 14% humidity, 14.9psia, 13.832V, 0.296A, 42.513Ah, 4.10W",Subject: "Started standAloneWCR_010224 phase initialPurge; standAloneWCR([nil,100], $da)" @18:28:10.48
Starting initialPurge @18:28:10.50 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @18:28:10.58 Hand.open @18:28:11.36 Elbow.reconfigure :tight @18:28:11.41 Elbow.seek FlushPuck::Garage @18:28:13.25 Elbow.reconfigure :loose @18:28:13.37 Forearm.reconfigure :loose @18:28:13.41 Forearm.seek :garage @18:28:14.36 Hand.close @18:28:14.84 upsync @18:28:15.12
Forearm.seek :retract @18:28:16.08 Elbow.reconfigure :tight @18:28:16.13 Elbow.seek CC @18:28:19.06 Elbow.reconfigure :loose @18:28:19.17 Forearm.reconfigure :tight @18:28:19.22 Forearm.seek CC @18:28:20.47 CC.seek :closed @18:28:25.01 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.39 @18:28:25.08 Hand.open @18:28:25.80 Forearm.reconfigure :loose @18:28:25.84 Forearm.seek :retract @18:28:26.80 Flushing the Collection Valve::Manifold @18:28:26.92 CSV.dial CSR @18:28:28.30 Pulling 5ml of CSR.flush @18:28:28.31 CSR.select :flush @18:28:28.42 CS.reconfigure :default @18:28:28.47 CS.seek 5ml @18:28:42.69 delay 5 seconds @18:28:47.70 CSR.select :air @18:28:47.88 CS.reconfigure :fast @18:28:47.92 CS.seek 7.5ml @18:28:54.59 CSV.dial :waste @18:28:55.85 CS.reconfigure :default @18:28:55.89 CS.seek 0ml @18:29:15.91 Purging external Intake @18:29:16.03 CSV.dial :flush @18:29:16.49 CS.reconfigure :fast @18:29:16.53 CS.seek 10ml @18:29:40.81 delay 5 seconds @18:29:45.83 CSV.dial CBV @18:29:45.86
CBV.dial CSV @18:29:46.61 CTV.dial :intake @18:29:47.39 Intake.select :open @18:29:47.50 CS.seek 0ml @18:30:10.96 Intake.select :close @18:30:11.07 CTV.dial :air @18:30:11.84 Flushing upper manifold @18:30:11.96 CSV.dial :flush @18:30:12.69 CS.seek 5ml @18:30:25.53 delay 5 seconds @18:30:30.55 CSV.dial :air @18:30:31.04 CS.seek 7ml @18:30:36.50 CSV.dial CBV @18:30:36.52
CBV.dial CSV @18:30:37.53 CTV.dial :air @18:30:37.61 CS.seek 0ml @18:30:54.03 delay 7 seconds @18:31:01.05 CS.seek 3ml @18:31:09.03 CSV.dial :waste @18:31:09.49 CS.seek 0ml @18:31:17.23 CSV.dial :flush @18:31:17.67 CS.seek 3ml @18:31:25.71 delay 5 seconds @18:31:30.73 CSV.dial :air @18:31:31.23 CS.seek 5ml @18:31:36.84 CSV.dial :air @18:31:37.02 CS.seek 0ml @18:31:49.24 delay 7 seconds @18:31:56.27 CS.seek 3ml @18:32:04.31 CSV.dial :waste @18:32:05.03 CS.seek 0ml @18:32:12.65 Garaging FlushPuck in Collection Clamp @18:32:12.73 Elbow.reconfigure :tight @18:32:12.77 Elbow.seek CC @18:32:13.04 Elbow.reconfigure :loose @18:32:13.14 Forearm.seek CC @18:32:14.11 Hand.close @18:32:14.74 CC.seek :open @18:32:19.76 Forearm.seek :retract @18:32:20.74 Elbow.reconfigure :tight @18:32:20.78 Elbow.seek :garage @18:32:23.70 Elbow.reconfigure :loose @18:32:23.80 Forearm.reconfigure :tight @18:32:23.84 Forearm.seek :garage @18:32:24.92 Hand.open @18:32:25.54 Forearm.reconfigure :loose @18:32:25.59 Forearm.seek :retract @18:32:26.59 Starting stand-alone da WCR @18:32:26.60 da wcr sampling at most 100ml @18:32:26.64 Whole Cell Archive sampling 100ml @18:32:26.65 DAfans.email "Started stand-alone da WCR in tube 2 at 18:32:26UTC02-Jan-24\n{2 => 1}\nCTD data unavailable\nCan@18:28:10, 25% flow, 22.0C, 14% humidity, 14.9psia, 13.832V, 0.296A, 42.513Ah, 4.10W",Subject: "da wcr sampling at most 100ml" @18:32:26.67 Loading wcr puck from tube 2 into Collection Clamp @18:32:26.94 SC.reconfigure :tight @18:32:27.01 SC.seek 2 @18:32:31.08 upsync @18:32:33.73 SC.configure :limp @18:32:33.97 Elbow.reconfigure :tight @18:32:34.02 Elbow.seek 2 @18:32:35.96 Forearm.reconfigure :tight @18:32:36.01 Forearm.seek 2 + 450 counts @18:32:37.02 SE.reconfigure :pick @18:32:37.08 SE.seek :beamBreak @18:32:40.19 SE.configure :default @18:32:40.35 Elbow.seek 2 @18:32:40.55 Forearm.seek 2 + 300 counts @18:32:40.75 Forearm.reconfigure :loose @18:32:40.80 Hand.close @18:32:41.38 SE.seek :down @18:32:42.02 Forearm.seek :retract @18:32:42.99 Elbow.seek CC @18:32:44.64 SE.stop @18:32:44.66 Elbow.reconfigure :loose @18:32:44.80 Forearm.reconfigure :tight @18:32:44.85 Forearm.seek CC @18:32:46.07 CC.seek :closed @18:32:50.60 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.18 @18:32:50.61 Hand.open @18:32:51.29 Forearm.reconfigure :loose @18:32:51.34 Forearm.seek :retract @18:32:52.33 collectSample 100ml w/bubble point of 35psi @18:32:52.34 Priming 7ml @18:32:52.35 Intake.select :close @18:32:52.36 Exhaust.select :close @18:32:52.38 SS.hold @18:32:52.59 Calibrating pressure and temperature sensors @18:32:52.62 CTV.dial :air @18:32:52.70 SSV.dial :spit,avoiding: :exhaust @18:32:52.72 SS.reconfigure :fast @18:32:52.77 SS.seek 5ml @18:32:55.73 SSV.dial CBV @18:32:55.75 CBV.dial SSV @18:32:57.10 delay 3.8 seconds @18:33:01.03 Sensor limits: Intake max 110.7psia; Outlet max 105.3psia @18:33:01.04 SSV.dial :spit @18:33:02.36 SS.seek 0ml @18:33:05.23 SSV.dial CBV @18:33:06.54 CTV.dial :intake @18:33:07.32 SSV.dial CBV @18:33:07.35 CBV.dial SSV @18:33:07.54 Allowing 40s for 7.0ml of remaining 7.0ml @18:33:07.55 Intake.select :open @18:33:09.72 Intake @15.5psia, vacuum from 10.0 to 13.0psi @18:33:09.74 SS.reconfigure :inhaling 2.20ml/s @18:33:09.78 SS.seek 7ml @18:33:17.25 Stroke completed -- waiting for pressure to equalize ... @18:33:17.27 SS.reconfigure :exhale @18:33:17.31 SS.hold @18:33:33.82 Intake.select :close @18:33:33.97 SSV.dial :exhaust @18:33:34.73 Exhaust.select :open @18:33:34.84 SS.seek 0ml @18:33:38.86 Exhaust.select :close @18:33:38.97 Sampling 100.0ml @18:33:38.98 Intake.select :close @18:33:39.00 Exhaust.select :close @18:33:39.01 SS.reconfigure :default @18:33:39.05 SS.hold @18:33:39.26 CTV.dial :intake @18:33:39.43 SSV.dial CBV @18:33:39.48 CBV.dial SSV @18:33:40.20 Allowing 130s for 25.0ml of remaining 100.0ml @18:33:40.21 Intake.select :open @18:33:42.37 Intake @15.6psia, vacuum from 10.0 to 13.0psi @18:33:42.39 SS.reconfigure :inhaling 2.20ml/s @18:33:42.44 SS.seek 25ml @18:33:51.50 Vacuum too high -- waiting up to 40 seconds @18:33:51.52 SS.reconfigure :exhale @18:33:51.57 SS.hold @18:33:56.36 Intake @15.5psia, vacuum from 10.0 to 13.0psi @18:33:56.38 SS.reconfigure :inhaling 1.76ml/s @18:33:56.42 SS.seek 25ml @18:34:00.66 Vacuum too high -- waiting up to 40 seconds @18:34:00.67 SS.reconfigure :exhale @18:34:00.73 SS.hold @18:34:41.22 SS.reconfigure :default @18:34:41.27 SS.hold @18:34:41.34 Intake.select :close @18:34:41.50 ShallowSampler::Clogged in WCR -- Intake @14.8psia, & Outlet @2.4psia -- no progress after 17.9ml @18:34:41.51 SSV.dial :exhaust @18:34:42.26 Exhaust.select :open @18:34:42.37 SS.reconfigure :exhale @18:34:42.42 SS.seek 0ml @18:34:49.49 Exhaust.select :close @18:34:49.60 Sampled 17.9ml @18:34:49.62 Drying puck before fixing for 5:00 @18:34:49.63 CBV.dial :waste2b @18:34:50.38 delay 1:00 @18:35:50.41 CSV.dial :air @18:35:51.26 CS.seek 10ml @18:36:15.56 Pumping wcr puck from tube 2 up to 35psi... @18:36:15.57 CBV.dial SSV @18:36:16.84 CBV.dial between CSV and waste2b @18:36:17.80 CSV.dial between air and CTV @18:36:18.12 CS.seek 7.29ml @18:36:25.06 CTV.dial CSV,avoiding: :air @18:36:25.08 CSV.dial CTV @18:36:25.92 CS.reconfigure :pressurize @18:36:25.97 CS.seek 0ml @18:36:45.18 CSV.dial :air @18:36:45.62 CS.reconfigure :fast @18:36:45.67 CS.seek 10ml @18:37:09.80 CSV.dial between air and CTV @18:37:10.12 CS.seek 2.924ml @18:37:26.96 CTV.dial CSV,avoiding: :air @18:37:27.35 CSV.dial CTV @18:37:27.76 CS.reconfigure :pressurize @18:37:27.81 CS.seek 0ml @18:37:29.26 CBV.dial :waste2b @18:37:29.59 CS.reconfigure :default @18:37:29.64 CS.seek 0ml @18:37:36.90 delay 1:00 @18:38:36.92 CTV.dial :air,avoiding: :intake @18:38:38.23 Fix #1 of 2 (with RNAlater) for 2:30 @18:38:38.24 CSV.dial CSR @18:38:38.68 Pulling 2ml of CSR.RNAlater @18:38:38.69 CSR.select :RNAlater @18:38:38.81 CS.seek 2ml @18:38:45.14 delay 5 seconds @18:38:50.17 CSR.select :air @18:38:50.28 CS.reconfigure :fast @18:38:50.33 CS.seek 10ml @18:39:09.83 Pumping wcr puck from tube 2 up to 35psi... @18:39:09.85 CBV.dial SSV @18:39:11.09 CBV.dial between CSV and waste2b @18:39:12.05 CSV.dial between air and CTV @18:39:12.65 CS.seek 7.324ml @18:39:19.53 CTV.dial CSV,avoiding: :air @18:39:19.55 CSV.dial CTV @18:39:20.93 CS.reconfigure :pressurize @18:39:20.98 CS.seek 0ml @18:39:36.82 delay 2:30 @18:42:06.85 CBV.dial :waste2b @18:42:07.18 CS.reconfigure :default @18:42:07.23 CS.seek 0ml @18:42:11.52 delay 1:00 @18:43:11.54 CTV.dial :air,avoiding: :intake @18:43:12.83 Fix #2 of 2 (with RNAlater) for 2:30 @18:43:12.85 CSV.dial CSR @18:43:13.30 Pulling 2ml of CSR.RNAlater @18:43:13.31 CSR.select :RNAlater @18:43:13.42 CS.seek 2ml @18:43:19.36 delay 5 seconds @18:43:24.38 CSR.select :air @18:43:24.50 CS.reconfigure :fast @18:43:24.55 CS.seek 10ml @18:43:44.08 Pumping wcr puck from tube 2 up to 35psi... @18:43:44.10 CBV.dial SSV @18:43:45.33 CBV.dial between CSV and waste2b @18:43:46.29 CSV.dial between air and CTV @18:43:47.01 CS.seek 7.256ml @18:43:54.21 CTV.dial CSV,avoiding: :air @18:43:54.23 CSV.dial CTV @18:43:55.57 CS.reconfigure :pressurize @18:43:56.06 CS.seek 0ml @18:44:10.41 delay 2:30 @18:46:40.44 CBV.dial :waste2b @18:46:40.77 CS.reconfigure :default @18:46:40.82 CS.seek 0ml @18:46:46.29 delay 1:00 @18:47:46.32 CTV.dial :air,avoiding: :intake @18:47:47.68 Storing wcr puck in Collection Clamp to tube 1 @18:47:47.86 SC.reconfigure :tight @18:47:47.91 SC.seek 1 @18:47:51.55 SC.configure :limp @18:47:51.77 Elbow.reconfigure :tight @18:47:51.82 Elbow.seek 1 @18:47:53.54 Forearm.reconfigure :tight @18:47:53.59 Forearm.seek 1 + 450 counts @18:47:54.64 SE.reconfigure :pick @18:47:54.70 SE.seek :beamBreak @18:47:57.53 SE.configure :default @18:47:57.59 SE.seek :place @18:47:58.14 Forearm.reconfigure :loose @18:47:58.19 Forearm.seek :retract @18:47:59.13 Elbow.seek CC @18:48:00.79 Elbow.reconfigure :loose @18:48:00.89 Forearm.seek CC @18:48:01.90 Hand.close @18:48:02.47 CC.seek :open @18:48:07.21 Forearm.seek :retract @18:48:08.21 Elbow.reconfigure :tight @18:48:08.26 Elbow.seek 1 @18:48:10.24 Elbow.reconfigure :limp @18:48:10.29 Forearm.seek 1 @18:48:11.73 Elbow.hold @18:48:11.80 Elbow.reconfigure :tight @18:48:11.85 Hand.open @18:48:12.59 Forearm.force -20 @18:48:12.63 Forearm.seek 1 + 310 counts @18:48:12.93 Forearm.reconfigure :tight @18:48:12.99 Forearm.seek 1 + 450 counts @18:48:13.25 SE.seek :down @18:48:14.38 Forearm.reconfigure :loose @18:48:14.43 Forearm.seek :retract @18:48:15.96 SE.stop @18:48:16.00
Starting finalPurge @18:48:16.02 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @18:48:16.20 Elbow.seek FlushPuck::Garage @18:48:18.06 Elbow.reconfigure :loose @18:48:18.16 Forearm.seek :garage @18:48:19.19 Hand.close @18:48:19.96 Forearm.seek :retract @18:48:20.92 Elbow.reconfigure :tight @18:48:20.97 Elbow.seek CC @18:48:23.92 Elbow.reconfigure :loose @18:48:24.01 Forearm.reconfigure :tight @18:48:24.06 Forearm.seek CC @18:48:25.32 CC.seek :closed @18:48:30.22 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.34 @18:48:30.36 Hand.open @18:48:31.03 Forearm.reconfigure :loose @18:48:31.08 Forearm.seek :retract @18:48:32.13 Flushing the Collection Valve::Manifold @18:48:32.26 CSV.dial CSR @18:48:32.81 Pulling 5ml of CSR.flush CSR.select :flush @18:48:32.93 CS.seek 5ml @18:48:46.60 delay 5 seconds @18:48:51.62 CSR.select :air @18:48:51.79 CS.reconfigure :fast @18:48:51.84 CS.seek 7.5ml @18:48:58.44 CSV.dial :waste @18:48:59.70 CS.reconfigure :default @18:48:59.75 CS.seek 0ml @18:49:19.76 Flushing lower manifold's waste2b port @18:49:19.88 CSV.dial :flush @18:49:20.34 CS.reconfigure :fast @18:49:20.39 CS.seek 5ml @18:49:32.98 delay 5 seconds @18:49:38.00 CSV.dial :air @18:49:38.49 CS.seek 7.001ml @18:49:44.01 CSV.dial CTV @18:49:44.03
CTV.dial CSV @18:49:45.38 CBV.dial :waste2b @18:49:45.47 CS.seek 0ml @18:50:01.92 Disinfecting external Intake with kill @18:50:02.03 CSV.dial CSR @18:50:02.48 Pulling 8ml of CSR.kill CSR.select :kill @18:50:02.60 CS.seek 8ml @18:50:22.16 delay 5 seconds @18:50:27.18 CSR.select :air @18:50:27.33 CS.seek 10ml @18:50:32.89 CSV.dial CBV @18:50:32.91
CBV.dial CSV @18:50:33.94 CTV.dial :intake @18:50:34.69 Intake.select :open @18:50:34.81 CS.seek 2ml @18:50:53.63 Intake.select :close @18:50:53.74 CTV.dial :air @18:50:54.46 CS.reconfigure :default @18:50:54.51 CS.seek 0ml @18:51:00.63 CSV.dial CSR @18:51:01.63 Pulling 5ml of CSR.flush @18:51:01.64 CSR.select :flush @18:51:01.75 CS.seek 5ml @18:51:15.35 delay 5 seconds @18:51:20.37 CSR.select :air @18:51:20.49 CS.seek 10ml @18:51:34.16 CSV.dial CBV @18:51:35.17 CS.seek 0ml @18:52:01.60 Garaging FlushPuck in Collection Clamp @18:52:02.11 Elbow.reconfigure :tight @18:52:02.17 Elbow.seek CC @18:52:02.45 Elbow.reconfigure :loose @18:52:02.55 Forearm.seek CC @18:52:03.56 Hand.close @18:52:04.22 CC.seek :open @18:52:08.84 Forearm.seek :retract @18:52:09.81 Elbow.reconfigure :tight @18:52:09.86 Elbow.seek :garage @18:52:12.76 Elbow.reconfigure :loose @18:52:12.86 Forearm.reconfigure :tight @18:52:12.90 Forearm.seek :garage @18:52:13.99 Hand.open @18:52:14.65 Forearm.reconfigure :loose @18:52:14.70 Forearm.seek :retract @18:52:15.68 Completed [initialPurge; standAloneWCR([nil,100], $da)] begun 18:28:10 @18:52:15.73 Asleep after completing standAloneWCR_010224 assays @18:52:15.75 GoodNews.email "{1 => 1,\n 2 => 0}\nCTD data unavailable\nCan@18:47:24, 26% flow, 22.0C, 14% humidity, 14.9psia, 13.817V, 0.453A, 42.651Ah, 6.27W\nSampled 17.9ml",Subject: "Asleep after completing standAloneWCR_010224 assays" @18:52:20.15 upsync + ESPwhich standAloneWCR_010224.rb + fn=standAloneWCR_010224.rb + IFS=: + set /home/esp/esp2/mission . + fullPath=/home/esp/esp2/mission/standAloneWCR_010224.rb + [ -f /home/esp/esp2/mission/standAloneWCR_010224.rb ] + realpath /home/esp/esp2/mission/standAloneWCR_010224.rb + return 0 + scriptPath=/home/esp/esp2local/mission/standAloneWCR_010224.rb + exec ruby /home/esp/esp2/lib/esp.rb /home/esp/esp2local/mission/standAloneWCR_010224.rb @18:00:19.37UTC03-Jan-24
ESP2 v3.42 I2C Gateway v3.20 for core bus @18:00:21.96 Configuration for short puck jake 1/11/21 by brent@mbari.org /home/esp/esp2/type/shallow/jake/configure.rb @18:00:25.86 Polling Can every 10:00 @18:00:25.87 Cleanly exited at 17:55:30UTC03-Jan-24 @18:00:25.92 Loading /home/esp/esp2local/mission/standAloneWCR_010224.rb mission @18:00:25.98 Preparing to sleep until + 00:00:05 @18:00:26.00 GoodNews.email "Starting standAloneWCR_010224 w/pucks in tube 2\n{2 => 1}\nCTD data unavailable\nCan@18:00:25, 26% flow, 20.6C, 18% humidity, 14.9psia, 13.812V, 0.290A, 0.075Ah, 4.01W",Subject: "standAloneWCR_010224 begun" @18:00:26.04
Asleep til + 00:00:05 @18:00:26.07 Continuing at 18:00 @18:00:26.33 Gate.power :core,:OFF @18:00:26.34 Gate.power :raw,:OFF @18:00:26.35 Shutdown @18:00:29.16 GoodNews.email "Then, in tube 2: initialPurge; standAloneWCR([nil,100], $da)\n/home/esp/esp2local/mission/standAloneWCR_010224.rb:10\n{2 => 1}\nCTD data unavailable\nCan@18:00:26, 25% flow, 20.6C, 18% humidity, 14.9psia, 13.808V, 0.290A, 0.075Ah, 4.01W",Subject: "Asleep until 18:00" @18:00:30.75 upsync @18:00:30.97
Powering core bus @18:00:33.57 upsync @18:00:36.48
Gate.power :raw,:ON @18:00:37.31 CS.configure :default @18:00:37.36 PS.configure :default @18:00:37.41 SS.configure :default @18:00:37.45 CC.configure :default @18:00:37.51 PC.configure :default @18:00:37.58 Forearm.configure :loose @18:00:37.63 Elbow.configure :loose @18:00:37.70 SC.configure :loose @18:00:37.75 SE.configure :default @18:00:37.80 CH.configure :default @18:00:37.84 PH.configure :default @18:00:37.88 All dwarf controllers running firmware version 3.85 @18:00:37.99 Valve.reset @18:00:38.52 Intake.select :close @18:00:38.64 Exhaust.select :close @18:00:38.68 CTV.dial :air @18:00:38.78 PSV.dial :air @18:00:38.80 CSV.dial :waste1 @18:00:38.99 PS.home.seek 0ml @18:00:39.01 SSV.dial :spit,avoiding: :exhaust @18:00:39.03
Forearm.home.seek :retract @18:00:39.39 CS.reconfigure :fast @18:00:39.43 SS.home.seek 0ml @18:00:39.53 CS.home.seek 0ml @18:00:39.86 Hand.close @18:00:39.89
Elbow.reconfigure :loose @18:00:39.98 Elbow.jog 1500 @18:00:40.47 Elbow.reconfigure :loose @18:00:40.51 Elbow.home.seek :clear @18:00:44.92 SE.home.seek :down @18:00:45.59 SC.reconfigure :tight @18:00:45.64 SC.home.seek :initial @18:00:47.40 PS.stop @18:01:10.80
SC.configure :limp @18:01:10.86 Forearm.seek :retract @18:01:10.92 Elbow.seek :hammer @18:01:11.97 Forearm.reconfigure :tight @18:01:12.02 Forearm.seek retracted @18:01:12.39 PC.home.seek :open @18:01:12.42
CC.home.seek :open @18:01:15.83 Phase [initialPurge; standAloneWCR([nil,100], $da)] @18:01:15.85 Notice.email "At 18:01:15 began in tube 2: initialPurge; standAloneWCR([nil,100], $da)\n/home/esp/esp2local/mission/standAloneWCR_010224.rb:10\n{2 => 1}\nCTD data unavailable\nCan@18:01:15, 25% flow, 20.6C, 18% humidity, 14.9psia, 13.656V, 0.260A, 0.080Ah, 3.55W",Subject: "Started standAloneWCR_010224 phase initialPurge; standAloneWCR([nil,100], $da)" @18:01:15.88
Starting initialPurge @18:01:15.89 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @18:01:15.97 Hand.open @18:01:16.67 Elbow.reconfigure :tight @18:01:16.72 Elbow.seek FlushPuck::Garage + ESPwhich standAloneWCR_010224.rb + fn=standAloneWCR_010224.rb + IFS=: + set /home/esp/esp2/mission . + fullPath=/home/esp/esp2/mission/standAloneWCR_010224.rb + [ -f /home/esp/esp2/mission/standAloneWCR_010224.rb ] + realpath /home/esp/esp2/mission/standAloneWCR_010224.rb + return 0 + scriptPath=/home/esp/esp2local/mission/standAloneWCR_010224.rb + exec ruby /home/esp/esp2/lib/esp.rb /home/esp/esp2local/mission/standAloneWCR_010224.rb @18:15:04.89UTC03-Jan-24
ESP2 v3.42 I2C Gateway v3.20 for core bus @18:15:07.60 Configuration for short puck jake 1/11/21 by brent@mbari.org /home/esp/esp2/type/shallow/jake/configure.rb @18:15:11.27 Polling Can every 10:00 @18:15:11.29 Cleanly exited at 18:14:43UTC03-Jan-24 @18:15:11.34 Loading /home/esp/esp2local/mission/standAloneWCR_010224.rb mission @18:15:11.40 Preparing to sleep until + 00:00:05 @18:15:11.41 GoodNews.email "Starting standAloneWCR_010224 w/pucks in tube 2\n{2 => 1}\nCTD data unavailable\nCan@18:15:11, 26% flow, 20.9C, 17% humidity, 14.9psia, 13.583V, 0.327A, 0.049Ah, 4.44W",Subject: "standAloneWCR_010224 begun" @18:15:11.46
Asleep til + 00:00:05 @18:15:11.54 Continuing at 18:15 @18:15:11.74 Gate.power :core,:OFF @18:15:11.75 Gate.power :raw,:OFF @18:15:11.76 Shutdown @18:15:14.73 GoodNews.email "Then, in tube 2: initialPurge; standAloneWCR([nil,100], $da)\n/home/esp/esp2local/mission/standAloneWCR_010224.rb:10\n{2 => 1}\nCTD data unavailable\nCan@18:15:11, 26% flow, 20.9C, 17% humidity, 14.9psia, 13.583V, 0.321A, 0.049Ah, 4.35W",Subject: "Asleep until 18:15" @18:15:16.34 upsync @18:15:16.43
Powering core bus @18:15:19.19 upsync @18:15:22.28
Gate.power :raw,:ON @18:15:23.15 CS.configure :default @18:15:23.20 PS.configure :default @18:15:23.25 SS.configure :default @18:15:23.30 CC.configure :default @18:15:23.34 PC.configure :default @18:15:23.41 Forearm.configure :loose @18:15:23.46 Elbow.configure :loose @18:15:23.53 SC.configure :loose @18:15:23.58 SE.configure :default @18:15:23.62 CH.configure :default @18:15:23.66 PH.configure :default @18:15:23.70 All dwarf controllers running firmware version 3.85 @18:15:23.81 Valve.reset @18:15:24.09 Intake.select :close @18:15:24.13 Exhaust.select :close @18:15:24.14 CTV.dial :air @18:15:24.22 PSV.dial :air @18:15:24.27 CSV.dial :waste1 @18:15:24.43
Forearm.home.seek :retract @18:15:24.45 PS.home.seek 0ml @18:15:24.77 SSV.dial :spit,avoiding: :exhaust @18:15:24.95 CS.reconfigure :fast @18:15:24.99 SS.home.seek 0ml @18:15:25.08 CS.home.seek 0ml @18:15:25.15 Hand.close @18:15:25.18
Elbow.reconfigure :loose @18:15:25.27 Elbow.jog 1500 @18:15:25.82 Elbow.reconfigure :loose @18:15:25.90 Elbow.home.seek :clear @18:15:29.11 SE.home.seek :down @18:15:29.75 SC.reconfigure :tight @18:15:29.81 SC.home.seek :initial @18:15:32.86 PS.stop @18:15:48.69
SC.configure :limp @18:15:48.74 Forearm.seek :retract @18:15:48.81 Elbow.seek :hammer @18:15:49.88 Forearm.reconfigure :tight @18:15:49.93 Forearm.seek retracted @18:15:50.19 CC.home.seek :open @18:15:50.21 PC.home.seek :open @18:15:53.92
Phase [initialPurge; standAloneWCR([nil,100], $da)] @18:15:53.94 Notice.email "At 18:15:53 began in tube 2: initialPurge; standAloneWCR([nil,100], $da)\n/home/esp/esp2local/mission/standAloneWCR_010224.rb:10\n{2 => 1}\nCTD data unavailable\nCan@18:15:53, 25% flow, 20.9C, 18% humidity, 14.9psia, 13.647V, 0.266A, 0.053Ah, 3.63W",Subject: "Started standAloneWCR_010224 phase initialPurge; standAloneWCR([nil,100], $da)" @18:15:53.96
Starting initialPurge @18:15:53.98 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @18:15:54.05 Hand.open @18:15:54.76 Elbow.reconfigure :tight @18:15:54.81 Elbow.seek FlushPuck::Garage @18:15:56.62 Elbow.reconfigure :loose @18:15:56.73 Forearm.reconfigure :loose @18:15:56.77 Forearm.seek :garage @18:15:57.85 Hand.close @18:15:58.35 upsync @18:15:58.62
Forearm.seek :retract @18:15:59.64 Elbow.reconfigure :tight @18:15:59.69 Elbow.seek CC @18:16:02.61 Elbow.reconfigure :loose @18:16:02.72 Forearm.reconfigure :tight @18:16:02.77 Forearm.seek CC @18:16:03.97 CC.seek :closed @18:16:08.51 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.44 @18:16:08.58 Hand.open @18:16:09.30 Forearm.reconfigure :loose @18:16:09.35 Forearm.seek :retract @18:16:10.34 Flushing the Collection Valve::Manifold @18:16:10.46 CSV.dial CSR @18:16:11.74 Pulling 5ml of CSR.flush @18:16:11.75 CSR.select :flush @18:16:11.86 CS.reconfigure :default @18:16:11.91 CS.seek 5ml @18:16:26.00 delay 5 seconds @18:16:31.02 CSR.select :air @18:16:31.19 CS.reconfigure :fast @18:16:31.24 CS.seek 7.5ml @18:16:38.10 CSV.dial :waste @18:16:39.38 CS.reconfigure :default @18:16:39.43 CS.seek 0ml @18:16:59.47 Purging external Intake @18:16:59.60 CSV.dial :flush @18:17:00.16 CS.reconfigure :fast @18:17:00.21 CS.seek 10ml @18:17:25.16 delay 5 seconds @18:17:30.18 CSV.dial CBV @18:17:30.22
CBV.dial CSV @18:17:30.94 CTV.dial :intake @18:17:31.72 Intake.select :open @18:17:31.84 CS.seek 0ml @18:17:55.66 Intake.select :close @18:17:55.76 CTV.dial :air @18:17:56.53 Flushing upper manifold @18:17:56.66 CSV.dial :flush @18:17:57.40 CS.seek 5ml @18:18:10.19 delay 5 seconds @18:18:15.21 CSV.dial :air @18:18:15.83 CS.seek 7.001ml @18:18:21.38 CSV.dial CBV @18:18:21.41
CBV.dial CSV @18:18:22.42 CTV.dial :air @18:18:22.51 CS.seek 0ml @18:18:39.09 delay 7 seconds @18:18:46.12 CS.seek 3ml @18:18:54.19 CSV.dial :waste @18:18:54.65 CS.seek 0ml @18:19:02.52 CSV.dial :flush @18:19:02.96 CS.seek 3ml @18:19:11.00 delay 5 seconds @18:19:16.02 CSV.dial :air @18:19:16.51 CS.seek 4.999ml @18:19:22.28 CSV.dial :air @18:19:22.36 CS.seek 0ml @18:19:34.53 delay 7 seconds @18:19:41.56 CS.seek 3ml @18:19:49.56 CSV.dial :waste @18:19:50.30 CS.seek 0ml @18:19:58.00 Garaging FlushPuck in Collection Clamp @18:19:58.07 Elbow.reconfigure :tight @18:19:58.12 Elbow.seek CC @18:19:58.39 Elbow.reconfigure :loose @18:19:58.49 Forearm.seek CC @18:19:59.55 Hand.close @18:20:00.12 CC.seek :open @18:20:04.81 Forearm.seek :retract @18:20:05.79 Elbow.reconfigure :tight @18:20:05.83 Elbow.seek :garage @18:20:08.70 Elbow.reconfigure :loose @18:20:08.80 Forearm.reconfigure :tight @18:20:08.85 Forearm.seek :garage @18:20:09.91 Hand.open @18:20:10.55 Forearm.reconfigure :loose @18:20:10.59 Forearm.seek :retract @18:20:11.61 Starting stand-alone da WCR @18:20:11.62 da wcr sampling at most 100ml @18:20:11.65 Whole Cell Archive sampling 100ml @18:20:11.67 DAfans.email "Started stand-alone da WCR in tube 2 at 18:20:11UTC03-Jan-24\n{2 => 1}\nCTD data unavailable\nCan@18:15:53, 25% flow, 20.9C, 18% humidity, 14.9psia, 13.647V, 0.266A, 0.053Ah, 3.63W",Subject: "da wcr sampling at most 100ml" @18:20:11.69 Loading wcr puck from tube 2 into Collection Clamp @18:20:11.98 SC.reconfigure :tight @18:20:12.04 SC.seek 2 @18:20:16.04 upsync @18:20:18.80 SC.configure :limp @18:20:19.04 Elbow.reconfigure :tight @18:20:19.09 Elbow.seek 2 @18:20:21.02 Forearm.reconfigure :tight @18:20:21.08 Forearm.seek 2 + 450 counts @18:20:22.08 SE.reconfigure :pick @18:20:22.15 SE.seek :beamBreak @18:20:25.18 SE.configure :default @18:20:25.34 Elbow.seek 2 @18:20:25.54 Forearm.seek 2 + 300 counts @18:20:25.83 Forearm.reconfigure :loose @18:20:25.89 Hand.close @18:20:26.94 SE.seek :down @18:20:27.15 Forearm.seek :retract @18:20:28.10 Elbow.seek CC @18:20:29.71 Elbow.reconfigure :loose @18:20:29.77 SE.stop @18:20:29.89 Forearm.reconfigure :tight @18:20:29.94 Forearm.seek CC @18:20:31.16 CC.seek :closed @18:20:35.68 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.25 @18:20:35.70 Hand.open @18:20:36.42 Forearm.reconfigure :loose @18:20:36.47 Forearm.seek :retract @18:20:37.50 collectSample 100ml w/bubble point of 35psi @18:20:37.52 Priming 7ml @18:20:37.53 Intake.select :close @18:20:37.54 Exhaust.select :close @18:20:37.55 SS.hold @18:20:37.76 Calibrating pressure and temperature sensors @18:20:37.79 CTV.dial :air @18:20:37.88 SSV.dial :spit,avoiding: :exhaust @18:20:37.97 SS.reconfigure :fast @18:20:38.02 SS.seek 5ml @18:20:41.22 SSV.dial CBV @18:20:41.26 CBV.dial SSV @18:20:42.58 delay 3.8 seconds @18:20:46.50 Sensor limits: Intake max 109.9psia; Outlet max 104.1psia @18:20:46.51 SSV.dial :spit @18:20:47.86 SS.seek 0ml @18:20:50.54 SSV.dial CBV @18:20:51.86 CTV.dial :intake @18:20:52.64 SSV.dial CBV @18:20:52.67 CBV.dial SSV @18:20:52.82 Allowing 40s for 7.0ml of remaining 7.0ml @18:20:52.84 Intake.select :open @18:20:55.00 Intake @15.6psia, vacuum from 10.0 to 13.0psi @18:20:55.02 SS.reconfigure :inhaling 2.20ml/s @18:20:55.07 SS.seek 7ml @18:21:02.49 Stroke completed -- waiting for pressure to equalize ... @18:21:02.51 SS.reconfigure :exhale @18:21:02.90 SS.hold @18:21:05.96 Intake.select :close @18:21:06.12 SSV.dial :exhaust @18:21:06.89 Exhaust.select :open @18:21:07.00 SS.seek 0ml @18:21:11.02 Exhaust.select :close @18:21:11.14 Sampling 100.0ml @18:21:11.15 Intake.select :close @18:21:11.16 Exhaust.select :close @18:21:11.17 SS.reconfigure :default @18:21:11.22 SS.hold @18:21:11.43 CTV.dial :intake @18:21:11.66 SSV.dial CBV @18:21:11.70 CBV.dial SSV @18:21:12.46 Allowing 130s for 25.0ml of remaining 100.0ml @18:21:12.48 Intake.select :open @18:21:14.64 Intake @15.6psia, vacuum from 10.0 to 13.0psi @18:21:14.66 SS.reconfigure :inhaling 2.20ml/s @18:21:14.71 SS.seek 25ml @18:21:30.60 Stroke completed -- waiting for pressure to equalize ... @18:21:30.61 SS.reconfigure :exhale @18:21:30.66 SS.hold @18:21:35.52 Intake.select :close @18:21:36.04 SSV.dial :exhaust @18:21:36.88 Exhaust.select :open @18:21:37.00 SS.seek 0ml @18:21:45.87 Exhaust.select :close @18:21:45.98 SSV.dial CBV @18:21:46.01 CBV.dial SSV @18:21:46.79 Allowing 130s for 25.0ml of remaining 75.0ml @18:21:46.80 Intake.select :open @18:21:48.97 Intake @15.6psia, vacuum from 10.0 to 13.0psi @18:21:48.99 SS.reconfigure :inhaling 2.20ml/s @18:21:49.04 SS.seek 25ml @18:22:05.05 Stroke completed -- waiting for pressure to equalize ... @18:22:05.07 SS.reconfigure :exhale @18:22:05.11 SS.hold @18:22:09.97 Intake.select :close @18:22:10.14 SSV.dial :exhaust @18:22:10.91 Exhaust.select :open @18:22:11.03 SS.seek 0ml @18:22:20.02 Exhaust.select :close @18:22:20.14 SSV.dial CBV @18:22:20.17 CBV.dial SSV @18:22:20.93 Allowing 130s for 25.0ml of remaining 50.0ml @18:22:20.94 Intake.select :open @18:22:23.11 Intake @15.6psia, vacuum from 10.0 to 13.0psi @18:22:23.13 SS.reconfigure :inhaling 2.20ml/s @18:22:23.18 SS.seek 25ml @18:22:38.71 Stroke completed -- waiting for pressure to equalize ... @18:22:38.72 SS.reconfigure :exhale @18:22:38.77 SS.hold @18:22:44.72 Intake.select :close @18:22:44.88 SSV.dial :exhaust @18:22:45.63 Exhaust.select :open @18:22:45.75 SS.seek 0ml @18:22:54.66 Exhaust.select :close @18:22:54.78 SSV.dial CBV @18:22:54.81 CBV.dial SSV @18:22:55.55 Allowing 130s for 25.0ml of remaining 25.0ml @18:22:55.57 Intake.select :open @18:22:57.74 Intake @15.6psia, vacuum from 10.0 to 13.0psi @18:22:57.75 SS.reconfigure :inhaling 2.20ml/s @18:22:57.80 SS.seek 25ml @18:23:13.13 Stroke completed -- waiting for pressure to equalize ... @18:23:13.14 SS.reconfigure :exhale @18:23:13.19 SS.hold @18:23:18.86 Intake.select :close @18:23:19.02 SSV.dial :exhaust @18:23:19.77 Exhaust.select :open @18:23:19.89 SS.seek 0ml @18:23:28.65 Exhaust.select :close @18:23:28.76 Sampled 100.0ml @18:23:28.77 Drying puck before fixing for 5:00 @18:23:28.78 CBV.dial :waste2b @18:23:29.53 delay 1:00 @18:24:29.56 CSV.dial :air @18:24:30.37 CS.seek 10ml @18:24:54.96 Pumping wcr puck from tube 2 up to 35psi... @18:24:54.98 CBV.dial SSV @18:24:56.24 CBV.dial between CSV and waste2b @18:24:57.19 CSV.dial between air and CTV @18:24:57.50 CS.seek 7.375ml @18:25:04.37 CTV.dial CSV,avoiding: :air @18:25:04.40 CSV.dial CTV @18:25:05.25 CS.reconfigure :pressurize @18:25:05.30 CS.seek 0ml @18:25:20.98 CBV.dial :waste2b @18:25:21.31 CS.reconfigure :default @18:25:21.36 CS.seek 0ml @18:25:25.71 delay 1:00 @18:26:25.74 CTV.dial :air,avoiding: :intake @18:26:27.08 Fix #1 of 2 (with RNAlater) for 2:30 @18:26:27.09 CSV.dial CSR @18:26:27.52 Pulling 2ml of CSR.RNAlater @18:26:27.53 CSR.select :RNAlater @18:26:27.65 CS.seek 2ml @18:26:33.55 delay 5 seconds @18:26:38.58 CSR.select :air @18:26:38.70 CS.reconfigure :fast @18:26:38.75 CS.seek 10ml @18:26:58.84 Pumping wcr puck from tube 2 up to 35psi... @18:26:58.86 CBV.dial SSV @18:27:00.11 CBV.dial between CSV and waste2b @18:27:01.05 CSV.dial between air and CTV @18:27:01.66 CS.seek 7.239ml @18:27:09.03 CTV.dial CSV,avoiding: :air @18:27:09.06 CSV.dial CTV @18:27:10.43 CS.reconfigure :pressurize @18:27:10.49 CS.seek 0ml @18:27:29.49 CSV.dial :air @18:27:29.95 CS.reconfigure :fast @18:27:30.00 CS.seek 10ml @18:27:54.70 CSV.dial between air and CTV @18:27:55.03 CS.seek 4.383ml @18:28:08.84 CTV.dial CSV,avoiding: :air @18:28:08.87 CSV.dial CTV @18:28:09.21 CS.reconfigure :pressurize @18:28:09.27 CS.seek 0ml @18:28:21.03 delay 2:30 @18:30:51.06 CBV.dial :waste2b @18:30:51.38 CS.reconfigure :default @18:30:51.43 CS.seek 0ml @18:30:52.55 delay 1:00 @18:31:52.58 CTV.dial :air,avoiding: :intake @18:31:53.90 Fix #2 of 2 (with RNAlater) for 2:30 @18:31:53.91 CSV.dial CSR @18:31:54.35 Pulling 2ml of CSR.RNAlater @18:31:54.36 CSR.select :RNAlater @18:31:54.47 CS.seek 2ml @18:32:00.42 delay 5 seconds @18:32:05.44 CSR.select :air @18:32:05.56 CS.reconfigure :fast @18:32:05.61 CS.seek 10ml @18:32:25.61 Pumping wcr puck from tube 2 up to 35psi... @18:32:25.63 CBV.dial SSV @18:32:26.88 CBV.dial between CSV and waste2b @18:32:27.86 CSV.dial between air and CTV @18:32:28.46 CS.seek 7.255ml @18:32:35.65 CTV.dial CSV,avoiding: :air @18:32:35.68 CSV.dial CTV @18:32:37.04 CS.reconfigure :pressurize @18:32:37.09 CS.seek 0ml @18:32:52.32 delay 2:30 @18:35:22.35 CBV.dial :waste2b @18:35:22.69 CS.reconfigure :default @18:35:22.74 CS.seek 0ml @18:35:27.33 delay 1:00 @18:36:27.36 CTV.dial :air,avoiding: :intake @18:36:28.73 Storing wcr puck in Collection Clamp to tube 1 @18:36:28.90 SC.reconfigure :tight @18:36:28.95 SC.seek 1 @18:36:32.57 SC.configure :limp @18:36:32.78 Elbow.reconfigure :tight @18:36:32.84 Elbow.seek 1 @18:36:34.53 Forearm.reconfigure :tight @18:36:34.58 Forearm.seek 1 + 450 counts @18:36:35.71 SE.reconfigure :pick @18:36:35.77 SE.seek :beamBreak @18:36:38.60 SE.configure :default @18:36:38.65 SE.seek :place @18:36:39.66 Forearm.reconfigure :loose @18:36:39.72 Forearm.seek :retract @18:36:40.69 Elbow.seek CC @18:36:42.28 Elbow.reconfigure :loose @18:36:42.39 Forearm.seek CC @18:36:43.36 Hand.close @18:36:43.99 CC.seek :open @18:36:48.72 Forearm.seek :retract @18:36:49.69 Elbow.reconfigure :tight @18:36:49.75 Elbow.seek 1 @18:36:51.49 Elbow.reconfigure :limp @18:36:51.55 Forearm.seek 1 @18:36:52.54 Elbow.hold @18:36:52.61 Elbow.reconfigure :tight @18:36:52.66 Hand.open @18:36:53.34 Forearm.force -20 @18:36:53.38 Forearm.seek 1 + 303 counts @18:36:53.67 Forearm.reconfigure :tight @18:36:53.73 Forearm.seek 1 + 450 counts @18:36:53.97 SE.seek :down @18:36:55.11 Forearm.reconfigure :loose @18:36:55.16 Forearm.seek :retract @18:36:56.68 SE.stop @18:36:56.72
Starting finalPurge @18:36:56.73 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @18:36:56.92 Elbow.seek FlushPuck::Garage @18:36:58.73 Elbow.reconfigure :loose @18:36:58.83 Forearm.seek :garage @18:36:59.89 Hand.close @18:37:00.66 Forearm.seek :retract @18:37:01.66 Elbow.reconfigure :tight @18:37:01.71 Elbow.seek CC @18:37:04.65 Elbow.reconfigure :loose @18:37:04.75 Forearm.reconfigure :tight @18:37:04.80 Forearm.seek CC @18:37:06.09 CC.seek :closed @18:37:10.65 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.42 @18:37:10.73 Hand.open @18:37:11.41 Forearm.reconfigure :loose @18:37:11.46 Forearm.seek :retract @18:37:12.41 Flushing the Collection Valve::Manifold @18:37:12.54 CSV.dial CSR @18:37:12.98 Pulling 5ml of CSR.flush @18:37:12.99 CSR.select :flush @18:37:13.11 CS.seek 5ml @18:37:26.79 delay 5 seconds @18:37:31.81 CSR.select :air @18:37:31.98 CS.reconfigure :fast @18:37:32.03 CS.seek 7.5ml @18:37:38.85 CSV.dial :waste @18:37:40.14 CS.reconfigure :default @18:37:40.19 CS.seek 0ml @18:38:00.39 Flushing lower manifold's waste2b port @18:38:00.51 CSV.dial :flush @18:38:00.95 CS.reconfigure :fast @18:38:01.00 CS.seek 5ml @18:38:14.04 delay 5 seconds @18:38:19.06 CSV.dial :air @18:38:19.70 CS.seek 7ml @18:38:25.20 CSV.dial CTV @18:38:25.23
CTV.dial CSV @18:38:26.59 CBV.dial :waste2b @18:38:26.77 CS.seek 0ml @18:38:43.83 Disinfecting external Intake with kill @18:38:44.40 CSV.dial CSR @18:38:44.84 Pulling 8ml of CSR.kill @18:38:44.85 CSR.select :kill @18:38:44.97 CS.seek 8ml @18:39:05.17 delay 5 seconds @18:39:10.19 CSR.select :air @18:39:10.31 CS.seek 10ml @18:39:16.11 CSV.dial CBV @18:39:16.14
CBV.dial CSV @18:39:17.20 CTV.dial :intake @18:39:17.98 Intake.select :open @18:39:18.09 CS.seek 2ml @18:39:37.26 Intake.select :close @18:39:37.36 CTV.dial :air @18:39:38.12 CS.reconfigure :default @18:39:38.17 CS.seek 0ml @18:39:44.13 CSV.dial CSR @18:39:45.28 Pulling 5ml of CSR.flush CSR.select :flush @18:39:45.40 CS.seek 5ml @18:39:59.20 delay 5 seconds @18:40:04.22 CSR.select :air @18:40:04.34 CS.seek 10ml @18:40:17.91 CSV.dial CBV @18:40:18.92 CS.seek 0ml @18:40:45.32 Garaging FlushPuck in Collection Clamp @18:40:45.39 Elbow.reconfigure :tight @18:40:45.44 Elbow.seek CC @18:40:45.70 Elbow.reconfigure :loose @18:40:45.80 Forearm.seek CC @18:40:46.89 Hand.close @18:40:47.47 CC.seek :open @18:40:52.09 Forearm.seek :retract @18:40:53.10 Elbow.reconfigure :tight @18:40:53.14 Elbow.seek :garage @18:40:56.06 Elbow.reconfigure :loose @18:40:56.16 Forearm.reconfigure :tight @18:40:56.20 Forearm.seek :garage @18:40:57.20 Hand.open @18:40:57.91 Forearm.reconfigure :loose @18:40:57.96 Forearm.seek :retract @18:40:58.92 Completed [initialPurge; standAloneWCR([nil,100], $da)] begun 18:15:53 @18:40:58.97 Asleep after completing standAloneWCR_010224 assays @18:40:58.99 GoodNews.email "{1 => 1,\n 2 => 0}\nCTD data unavailable\nCan@18:35:13, 25% flow, 21.1C, 17% humidity, 14.9psia, 13.661V, 0.447A, 0.197Ah, 6.11W\nSampled 100.0ml",Subject: "Asleep after completing standAloneWCR_010224 assays" @18:41:03.66 upsync + ESPwhich standAloneWCR_010224.rb + fn=standAloneWCR_010224.rb + IFS=: + set /home/esp/esp2/mission . + fullPath=/home/esp/esp2/mission/standAloneWCR_010224.rb + [ -f /home/esp/esp2/mission/standAloneWCR_010224.rb ] + realpath /home/esp/esp2/mission/standAloneWCR_010224.rb + return 0 + scriptPath=/home/esp/esp2local/mission/standAloneWCR_010224.rb + exec ruby /home/esp/esp2/lib/esp.rb /home/esp/esp2local/mission/standAloneWCR_010224.rb @15:05:23.05UTC04-Jan-24
ESP2 v3.42 I2C Gateway v3.20 for core bus @15:05:25.86 Configuration for short puck jake 1/11/21 by brent@mbari.org /home/esp/esp2/type/shallow/jake/configure.rb @15:05:29.73 Polling Can every 10:00 @15:05:29.75 Cleanly exited at 15:04:49UTC04-Jan-24 @15:05:29.80 Loading /home/esp/esp2local/mission/standAloneWCR_010224.rb mission @15:05:29.85 Preparing to sleep until + 00:00:05 @15:05:29.89 GoodNews.email "Starting standAloneWCR_010224 w/pucks in tube 2\n{2 => 1}\nCTD data unavailable\nCan@15:05:29, 26% flow, 20.2C, 21% humidity, 14.9psia, 13.759V, 0.296A, 0.060Ah, 4.08W",Subject: "standAloneWCR_010224 begun" @15:05:29.93
Asleep til + 00:00:05 @15:05:29.95 Continuing at 15:05 @15:05:30.19 Gate.power :core,:OFF @15:05:30.21 Gate.power :raw,:OFF @15:05:30.22 Shutdown @15:05:33.03 GoodNews.email "Then, in tube 2: initialPurge; standAloneWCR([nil,100], $da)\n/home/esp/esp2local/mission/standAloneWCR_010224.rb:10\n{2 => 1}\nCTD data unavailable\nCan@15:05:29, 26% flow, 20.2C, 21% humidity, 14.9psia, 13.759V, 0.290A, 0.060Ah, 4.00W",Subject: "Asleep until 15:05" @15:05:34.59 upsync @15:05:34.83
Powering core bus @15:05:37.06 upsync @15:05:40.36
Gate.power :raw,:ON @15:05:41.68 CS.configure :default @15:05:41.73 PS.configure :default @15:05:41.78 SS.configure :default @15:05:41.82 CC.configure :default @15:05:41.87 PC.configure :default @15:05:41.94 Forearm.configure :loose @15:05:41.99 Elbow.configure :loose @15:05:42.06 SC.configure :loose @15:05:42.10 SE.configure :default @15:05:42.15 CH.configure :default @15:05:42.18 PH.configure :default @15:05:42.22 All dwarf controllers running firmware version 3.85 @15:05:42.33 Valve.reset @15:05:42.60 Intake.select :close @15:05:42.64 Exhaust.select :close @15:05:42.65 CTV.dial :air @15:05:42.73 PSV.dial :air @15:05:42.89 CSV.dial :waste1 @15:05:42.92
Forearm.home.seek :retract @15:05:42.94 PS.home.seek 0ml @15:05:43.41 SSV.dial :spit,avoiding: :exhaust @15:05:43.60 CS.reconfigure :fast @15:05:43.63 SS.home.seek 0ml @15:05:43.73 CS.home.seek 0ml @15:05:43.79 Hand.close @15:05:43.84
Elbow.reconfigure :loose @15:05:43.91 Elbow.jog 1500 @15:05:44.41 Elbow.reconfigure :loose @15:05:44.46 Elbow.home.seek :clear @15:05:47.70 SE.home.seek :down @15:05:48.31 SC.reconfigure :tight @15:05:48.36 SC.home.seek :initial @15:05:51.36 PS.stop @15:06:13.57
SC.configure :limp @15:06:13.63 Forearm.seek :retract @15:06:13.69 Elbow.seek :hammer @15:06:14.81 Forearm.reconfigure :tight @15:06:14.86 Forearm.seek retracted @15:06:15.24 CC.home.seek :open @15:06:15.26 PC.home.seek :open @15:06:18.79
Phase [initialPurge; standAloneWCR([nil,100], $da)] @15:06:18.81 Notice.email "At 15:06:18 began in tube 2: initialPurge; standAloneWCR([nil,100], $da)\n/home/esp/esp2local/mission/standAloneWCR_010224.rb:10\n{2 => 1}\nCTD data unavailable\nCan@15:06:18, 25% flow, 20.3C, 21% humidity, 14.9psia, 13.793V, 0.260A, 0.065Ah, 3.59W",Subject: "Started standAloneWCR_010224 phase initialPurge; standAloneWCR([nil,100], $da)" @15:06:18.83
Starting initialPurge @15:06:18.86 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @15:06:18.93 Hand.open @15:06:19.66 Elbow.reconfigure :tight @15:06:19.71 Elbow.seek FlushPuck::Garage @15:06:21.52 Elbow.reconfigure :loose @15:06:21.63 Forearm.reconfigure :loose @15:06:21.76 Forearm.seek :garage @15:06:22.80 Hand.close @15:06:23.10 upsync @15:06:23.52
Forearm.seek :retract @15:06:24.50 Elbow.reconfigure :tight @15:06:24.55 Elbow.seek CC @15:06:27.47 Elbow.reconfigure :loose @15:06:27.58 Forearm.reconfigure :tight @15:06:27.62 Forearm.seek CC @15:06:28.90 CC.seek :closed @15:06:33.48 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.36 @15:06:33.55 Hand.open @15:06:34.20 Forearm.reconfigure :loose @15:06:34.25 Forearm.seek :retract @15:06:35.22 Flushing the Collection Valve::Manifold @15:06:35.35 CSV.dial CSR @15:06:36.74 Pulling 5ml of CSR.flush CSR.select :flush @15:06:36.85 CS.reconfigure :default @15:06:36.90 CS.seek 5ml @15:06:50.56 delay 5 seconds @15:06:55.90 CSR.select :air @15:06:56.16 CS.reconfigure :fast @15:06:56.21 CS.seek 7.501ml @15:07:02.94 CSV.dial :waste @15:07:04.21 CS.reconfigure :default @15:07:04.25 CS.seek 0ml @15:07:24.22 Purging external Intake @15:07:24.34 CSV.dial :flush @15:07:24.80 CS.reconfigure :fast @15:07:24.85 CS.seek 10ml @15:07:49.50 delay 5 seconds @15:07:54.52 CSV.dial CBV @15:07:54.55
CBV.dial CSV @15:07:55.31 CTV.dial :intake @15:07:56.09 Intake.select :open @15:07:56.21 CS.seek 0ml @15:08:19.93 Intake.select :close @15:08:20.04 CTV.dial :air @15:08:20.83 Flushing upper manifold @15:08:20.95 CSV.dial :flush @15:08:21.69 CS.seek 5ml @15:08:34.50 delay 5 seconds @15:08:39.52 CSV.dial :air @15:08:40.01 CS.seek 7ml @15:08:45.66 CSV.dial CBV @15:08:45.69
CBV.dial CSV @15:08:46.70 CTV.dial :air @15:08:46.79 CS.seek 0ml @15:09:03.59 delay 7 seconds @15:09:10.62 CS.seek 3ml @15:09:18.75 CSV.dial :waste @15:09:19.21 CS.seek 0ml @15:09:27.11 CSV.dial :flush @15:09:27.55 CS.seek 3ml @15:09:35.65 delay 5 seconds @15:09:40.67 CSV.dial :air @15:09:41.29 CS.seek 5ml @15:09:46.84 CSV.dial :air @15:09:46.92 CS.seek 0ml @15:09:59.18 delay 7 seconds @15:10:06.21 CS.seek 3ml @15:10:14.25 CSV.dial :waste @15:10:14.97 CS.seek 0ml @15:10:22.69 Garaging FlushPuck in Collection Clamp @15:10:22.76 Elbow.reconfigure :tight @15:10:22.80 Elbow.seek CC @15:10:23.12 Elbow.reconfigure :loose @15:10:23.22 Forearm.seek CC @15:10:24.25 Hand.close @15:10:24.90 CC.seek :open @15:10:29.92 Forearm.seek :retract @15:10:30.89 Elbow.reconfigure :tight @15:10:30.94 Elbow.seek :garage @15:10:33.76 Elbow.reconfigure :loose @15:10:33.86 Forearm.reconfigure :tight @15:10:33.91 Forearm.seek :garage @15:10:34.90 Hand.open @15:10:35.58 Forearm.reconfigure :loose @15:10:35.62 Forearm.seek :retract @15:10:36.59 Starting stand-alone da WCR @15:10:36.61 da wcr sampling at most 100ml @15:10:36.64 Whole Cell Archive sampling 100ml @15:10:36.65 DAfans.email "Started stand-alone da WCR in tube 2 at 15:10:36UTC04-Jan-24\n{2 => 1}\nCTD data unavailable\nCan@15:06:18, 25% flow, 20.3C, 21% humidity, 14.9psia, 13.793V, 0.260A, 0.065Ah, 3.59W",Subject: "da wcr sampling at most 100ml" @15:10:36.69 Loading wcr puck from tube 2 into Collection Clamp @15:10:36.95 SC.reconfigure :tight @15:10:37.00 SC.seek 2 @15:10:40.64 upsync @15:10:43.73 SC.configure :limp @15:10:43.98 Elbow.reconfigure :tight @15:10:44.03 Elbow.seek 2 @15:10:46.05 Forearm.reconfigure :tight @15:10:46.10 Forearm.seek 2 + 450 counts @15:10:47.11 SE.reconfigure :pick @15:10:47.18 SE.seek :beamBreak @15:10:50.27 SE.configure :default @15:10:50.43 Elbow.seek 2 @15:10:50.62 Forearm.seek 2 + 300 counts @15:10:50.93 Forearm.reconfigure :loose @15:10:50.98 Hand.close @15:10:51.66 SE.seek :down @15:10:52.30 Forearm.seek :retract @15:10:53.25 Elbow.seek CC @15:10:54.43 SE.stop @15:10:54.94 Elbow.reconfigure :loose @15:10:55.04 Forearm.reconfigure :tight @15:10:55.09 Forearm.seek CC @15:10:56.39 CC.seek :closed @15:11:00.91 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.24 @15:11:00.92 Hand.open @15:11:01.58 Forearm.reconfigure :loose @15:11:01.63 Forearm.seek :retract @15:11:02.58 collectSample 100ml w/bubble point of 35psi @15:11:02.60 Priming 7ml @15:11:02.61 Intake.select :close @15:11:02.62 Exhaust.select :close @15:11:02.63 SS.hold @15:11:02.84 Calibrating pressure and temperature sensors @15:11:02.87 CTV.dial :air @15:11:02.88 SSV.dial :spit,avoiding: :exhaust @15:11:02.90 SS.reconfigure :fast @15:11:02.95 SS.seek 5ml @15:11:05.85 SSV.dial CBV @15:11:05.89 CBV.dial SSV @15:11:07.23 delay 3.8 seconds @15:11:11.16 Sensor limits: Intake max 107.9psia; Outlet max 102.3psia @15:11:11.17 SSV.dial :spit @15:11:12.48 SS.seek 0ml @15:11:15.38 SSV.dial CBV @15:11:16.71 CTV.dial :intake @15:11:17.51 SSV.dial CBV @15:11:17.55 CBV.dial SSV @15:11:17.62 Allowing 40s for 7.0ml of remaining 7.0ml @15:11:17.63 Intake.select :open @15:11:19.79 Intake @15.4psia, vacuum from 9.9 to 12.9psi @15:11:19.81 SS.reconfigure :inhaling 2.20ml/s @15:11:19.86 SS.seek 7ml @15:11:26.99 Stroke completed -- waiting for pressure to equalize ... @15:11:27.00 SS.reconfigure :exhale @15:11:27.05 SS.hold @15:11:30.28 Intake.select :close @15:11:30.43 SSV.dial :exhaust @15:11:31.18 Exhaust.select :open @15:11:31.30 SS.seek 0ml @15:11:35.43 Exhaust.select :close @15:11:35.54 Sampling 100.0ml @15:11:35.56 Intake.select :close @15:11:35.57 Exhaust.select :close @15:11:35.58 SS.reconfigure :default @15:11:35.62 SS.hold @15:11:35.83 CTV.dial :intake @15:11:35.90 SSV.dial CBV @15:11:35.92 CBV.dial SSV @15:11:36.68 Allowing 130s for 25.0ml of remaining 100.0ml @15:11:36.70 Intake.select :open @15:11:38.86 Intake @15.4psia, vacuum from 9.9 to 12.9psi @15:11:38.88 SS.reconfigure :inhaling 2.20ml/s @15:11:38.93 SS.seek 25ml @15:11:54.13 Stroke completed -- waiting for pressure to equalize ... @15:11:54.14 SS.reconfigure :exhale @15:11:54.19 SS.hold @15:11:59.06 Intake.select :close @15:11:59.23 SSV.dial :exhaust @15:11:59.98 Exhaust.select :open @15:12:00.09 SS.seek 0ml @15:12:09.02 Exhaust.select :close @15:12:09.13 SSV.dial CBV @15:12:09.15 CBV.dial SSV @15:12:09.98 Allowing 130s for 25.0ml of remaining 75.0ml @15:12:10.00 Intake.select :open @15:12:12.16 Intake @15.5psia, vacuum from 10.0 to 13.0psi @15:12:12.18 SS.reconfigure :inhaling 2.20ml/s @15:12:12.23 SS.seek 25ml @15:12:27.50 Stroke completed -- waiting for pressure to equalize ... @15:12:27.52 SS.reconfigure :exhale @15:12:27.56 SS.hold @15:12:33.21 Intake.select :close @15:12:33.36 SSV.dial :exhaust @15:12:34.12 Exhaust.select :open @15:12:34.23 SS.seek 0ml @15:12:43.11 Exhaust.select :close @15:12:43.23 SSV.dial CBV @15:12:43.25 CBV.dial SSV @15:12:44.01 Allowing 130s for 25.0ml of remaining 50.0ml @15:12:44.02 Intake.select :open @15:12:46.19 Intake @15.5psia, vacuum from 10.0 to 13.0psi @15:12:46.20 SS.reconfigure :inhaling 2.20ml/s @15:12:46.25 SS.seek 25ml @15:13:01.63 Stroke completed -- waiting for pressure to equalize ... @15:13:01.64 SS.reconfigure :exhale @15:13:01.69 SS.hold @15:13:07.32 Intake.select :close @15:13:07.48 SSV.dial :exhaust @15:13:08.22 Exhaust.select :open @15:13:08.34 SS.seek 0ml @15:13:17.41 Exhaust.select :close @15:13:17.52 SSV.dial CBV @15:13:17.57 CBV.dial SSV @15:13:18.29 Allowing 130s for 25.0ml of remaining 25.0ml @15:13:18.30 Intake.select :open @15:13:20.47 Intake @15.6psia, vacuum from 10.0 to 13.0psi @15:13:20.49 SS.reconfigure :inhaling 2.20ml/s @15:13:20.54 SS.seek 25ml @15:13:35.30 Stroke completed -- waiting for pressure to equalize ... @15:13:35.31 SS.reconfigure :exhale @15:13:35.36 SS.hold @15:13:41.87 Intake.select :close @15:13:42.02 SSV.dial :exhaust @15:13:42.77 Exhaust.select :open @15:13:42.89 SS.seek 0ml @15:13:51.83 Exhaust.select :close @15:13:51.95 Sampled 100.0ml @15:13:51.96 Drying puck before fixing for 5:00 @15:13:51.97 CBV.dial :waste2b @15:13:52.71 delay 1:00 @15:14:52.74 CSV.dial :air @15:14:53.48 CS.seek 10ml @15:15:18.12 Pumping wcr puck from tube 2 up to 35psi... @15:15:18.14 CBV.dial SSV @15:15:19.41 CBV.dial between CSV and waste2b @15:15:20.78 CSV.dial between air and CTV @15:15:21.09 CS.seek 7.322ml @15:15:28.13 CTV.dial CSV,avoiding: :air @15:15:28.14 CSV.dial CTV @15:15:28.95 CS.reconfigure :pressurize @15:15:29.00 CS.seek 0ml @15:15:48.24 CSV.dial :air @15:15:48.70 CS.reconfigure :fast @15:15:48.75 CS.seek 10ml @15:16:13.27 CSV.dial between air and CTV @15:16:13.59 CS.seek 3.355ml @15:16:29.56 CTV.dial CSV,avoiding: :air @15:16:29.58 CSV.dial CTV @15:16:29.92 CS.reconfigure :pressurize @15:16:29.97 CS.seek 0ml @15:16:33.54 CBV.dial :waste2b @15:16:33.87 CS.reconfigure :default @15:16:33.92 CS.seek 0ml @15:16:40.34 delay 1:00 @15:17:40.37 CTV.dial :air,avoiding: :intake @15:17:41.68 Fix #1 of 2 (with RNAlater) for 2:30 @15:17:41.70 CSV.dial CSR @15:17:42.13 Pulling 2ml of CSR.RNAlater @15:17:42.14 CSR.select :RNAlater @15:17:42.26 CS.seek 2ml @15:17:48.24 delay 5 seconds @15:17:53.26 CSR.select :air @15:17:53.38 CS.reconfigure :fast @15:17:53.43 CS.seek 10ml @15:18:13.18 Pumping wcr puck from tube 2 up to 35psi... @15:18:13.20 CBV.dial SSV @15:18:14.45 CBV.dial between CSV and waste2b @15:18:15.41 CSV.dial between air and CTV @15:18:16.01 CS.seek 7.271ml @15:18:23.09 CTV.dial CSV,avoiding: :air @15:18:23.11 CSV.dial CTV @15:18:24.48 CS.reconfigure :pressurize @15:18:24.53 CS.seek 0ml @15:18:43.64 CSV.dial :air @15:18:44.10 CS.reconfigure :fast @15:18:44.15 CS.seek 10ml @15:19:09.16 CSV.dial between air and CTV @15:19:09.54 CS.seek 4ml @15:19:24.09 CTV.dial CSV,avoiding: :air @15:19:24.11 CSV.dial CTV @15:19:24.42 CS.reconfigure :pressurize @15:19:24.47 CS.seek 0ml @15:19:33.20 delay 2:30 @15:22:03.22 CBV.dial :waste2b @15:22:03.55 CS.reconfigure :default @15:22:03.60 CS.seek 0ml @15:22:06.30 delay 1:00 @15:23:06.32 CTV.dial :air,avoiding: :intake @15:23:07.63 Fix #2 of 2 (with RNAlater) for 2:30 @15:23:07.64 CSV.dial CSR @15:23:08.08 Pulling 2ml of CSR.RNAlater @15:23:08.09 CSR.select :RNAlater @15:23:08.21 CS.seek 2ml @15:23:14.11 delay 5 seconds @15:23:19.13 CSR.select :air @15:23:19.25 CS.reconfigure :fast @15:23:19.30 CS.seek 10ml @15:23:39.11 Pumping wcr puck from tube 2 up to 35psi... @15:23:39.13 CBV.dial SSV @15:23:40.38 CBV.dial between CSV and waste2b @15:23:41.34 CSV.dial between air and CTV @15:23:41.94 CS.seek 7.238ml @15:23:49.15 CTV.dial CSV,avoiding: :air @15:23:49.17 CSV.dial CTV @15:23:50.54 CS.reconfigure :pressurize @15:23:50.59 CS.seek 0ml @15:24:05.85 delay 2:30 @15:26:35.87 CBV.dial :waste2b @15:26:36.21 CS.reconfigure :default @15:26:36.26 CS.seek 0ml @15:26:40.82 delay 1:00 @15:27:40.85 CTV.dial :air,avoiding: :intake @15:27:42.23 Storing wcr puck in Collection Clamp to tube 1 @15:27:42.40 SC.reconfigure :tight @15:27:42.46 SC.seek 1 @15:27:46.00 SC.configure :limp @15:27:46.22 Elbow.reconfigure :tight @15:27:46.27 Elbow.seek 1 @15:27:48.07 Forearm.reconfigure :tight @15:27:48.12 Forearm.seek 1 + 450 counts @15:27:49.58 SE.reconfigure :pick @15:27:49.64 SE.seek :beamBreak @15:27:52.45 SE.configure :default @15:27:52.51 SE.seek :place @15:27:53.06 Forearm.reconfigure :loose @15:27:53.11 Forearm.seek :retract @15:27:54.08 Elbow.seek CC @15:27:55.76 Elbow.reconfigure :loose @15:27:55.86 Forearm.seek CC @15:27:56.80 Hand.close @15:27:57.38 CC.seek :open @15:28:02.15 Forearm.seek :retract @15:28:03.14 Elbow.reconfigure :tight @15:28:03.19 Elbow.seek 1 @15:28:04.96 Elbow.reconfigure :limp @15:28:05.01 Forearm.seek 1 @15:28:06.02 Elbow.hold @15:28:06.08 Elbow.reconfigure :tight @15:28:06.13 Hand.open @15:28:06.87 Forearm.force -20 @15:28:06.91 Forearm.seek 1 + 305 counts @15:28:07.20 Forearm.reconfigure :tight @15:28:07.25 Forearm.seek 1 + 450 counts @15:28:07.52 SE.seek :down @15:28:08.65 Forearm.reconfigure :loose @15:28:08.70 Forearm.seek :retract @15:28:10.19 SE.stop @15:28:10.24
Starting finalPurge @15:28:10.25 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @15:28:10.88 Elbow.seek FlushPuck::Garage @15:28:12.68 Elbow.reconfigure :loose @15:28:12.78 Forearm.seek :garage @15:28:13.81 Hand.close @15:28:14.50 Forearm.seek :retract @15:28:15.52 Elbow.reconfigure :tight @15:28:15.57 Elbow.seek CC @15:28:18.49 Elbow.reconfigure :loose @15:28:18.59 Forearm.reconfigure :tight @15:28:18.64 Forearm.seek CC @15:28:19.90 CC.seek :closed @15:28:24.48 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.35 @15:28:24.55 Hand.open @15:28:25.19 Forearm.reconfigure :loose @15:28:25.23 Forearm.seek :retract @15:28:26.19 Flushing the Collection Valve::Manifold @15:28:26.32 CSV.dial CSR @15:28:26.76 Pulling 5ml of CSR.flush @15:28:26.77 CSR.select :flush @15:28:26.89 CS.seek 5ml @15:28:40.45 delay 5 seconds @15:28:45.46 CSR.select :air @15:28:45.63 CS.reconfigure :fast @15:28:45.68 CS.seek 7.5ml @15:28:52.48 CSV.dial :waste @15:28:53.76 CS.reconfigure :default @15:28:53.81 CS.seek 0ml @15:29:13.86 Flushing lower manifold's waste2b port @15:29:13.98 CSV.dial :flush @15:29:14.44 CS.reconfigure :fast @15:29:14.48 CS.seek 5ml @15:29:27.23 delay 5 seconds @15:29:32.25 CSV.dial :air @15:29:33.21 CS.seek 7ml @15:29:38.73 CSV.dial CTV @15:29:38.75
CTV.dial CSV @15:29:40.15 CBV.dial :waste2b @15:29:40.17 CS.seek 0ml @15:29:56.83 Disinfecting external Intake with kill @15:29:56.95 CSV.dial CSR @15:29:57.40 Pulling 8ml of CSR.kill @15:29:57.41 CSR.select :kill @15:29:57.53 CS.seek 8ml @15:30:17.29 delay 5 seconds @15:30:22.31 CSR.select :air @15:30:22.43 CS.seek 10ml @15:30:28.04 CSV.dial CBV @15:30:28.06
CBV.dial CSV @15:30:29.24 CTV.dial :intake @15:30:30.02 Intake.select :open @15:30:30.14 CS.seek 2ml @15:30:49.10 Intake.select :close @15:30:49.20 CTV.dial :air @15:30:49.95 CS.reconfigure :default @15:30:50.00 CS.seek 0ml @15:30:55.91 CSV.dial CSR @15:30:56.91 Pulling 5ml of CSR.flush @15:30:56.92 CSR.select :flush @15:30:57.04 CS.seek 5ml @15:31:10.88 delay 5 seconds @15:31:15.90 CSR.select :air @15:31:16.02 CS.seek 10ml @15:31:29.66 CSV.dial CBV @15:31:30.68 CS.seek 0ml @15:31:57.17 Garaging FlushPuck in Collection Clamp @15:31:57.23 Elbow.reconfigure :tight @15:31:57.28 Elbow.seek CC @15:31:57.54 Elbow.reconfigure :loose @15:31:57.64 Forearm.seek CC @15:31:58.67 Hand.close @15:31:59.25 CC.seek :open @15:32:03.88 Forearm.seek :retract @15:32:04.84 Elbow.reconfigure :tight @15:32:04.89 Elbow.seek :garage @15:32:07.83 Elbow.reconfigure :loose @15:32:07.93 Forearm.reconfigure :tight @15:32:07.98 Forearm.seek :garage @15:32:09.00 Hand.open @15:32:09.68 Forearm.reconfigure :loose @15:32:09.73 Forearm.seek :retract @15:32:10.72 Completed [initialPurge; standAloneWCR([nil,100], $da)] begun 15:06:18 @15:32:10.77 Asleep after completing standAloneWCR_010224 assays @15:32:11.23 GoodNews.email "{1 => 1,\n 2 => 0}\nCTD data unavailable\nCan@15:25:32, 26% flow, 20.5C, 21% humidity, 14.9psia, 13.691V, 0.429A, 0.206Ah, 5.88W\nSampled 100.0ml",Subject: "Asleep after completing standAloneWCR_010224 assays" @15:32:15.36 upsync + ESPwhich standAloneWCR_010224.rb + fn=standAloneWCR_010224.rb + IFS=: + set /home/esp/esp2/mission . + fullPath=/home/esp/esp2/mission/standAloneWCR_010224.rb + [ -f /home/esp/esp2/mission/standAloneWCR_010224.rb ] + realpath /home/esp/esp2/mission/standAloneWCR_010224.rb + return 0 + scriptPath=/home/esp/esp2local/mission/standAloneWCR_010224.rb + exec ruby /home/esp/esp2/lib/esp.rb /home/esp/esp2local/mission/standAloneWCR_010224.rb @13:46:19.08UTC05-Jan-24
ESP2 v3.42 I2C Gateway v3.20 for core bus @13:46:21.75 Configuration for short puck jake 1/11/21 by brent@mbari.org /home/esp/esp2/type/shallow/jake/configure.rb @13:46:25.43 Polling Can every 10:00 @13:46:25.49 Loading /home/esp/esp2local/mission/standAloneWCR_010224.rb mission @13:46:25.55 Preparing to sleep until + 00:00:05 @13:46:25.58 GoodNews.email "Starting standAloneWCR_010224 w/pucks in tube 2\n{2 => 1}\nCTD data unavailable\nCan@13:46:25, 26% flow, 21.3C, 11% humidity, 15.0psia, 13.769V, 0.351A, 6.507Ah, 4.83W",Subject: "standAloneWCR_010224 begun" @13:46:25.60
Asleep til + 00:00:05 @13:46:25.65 Continuing at 13:46 @13:46:25.89 Gate.power :core,:OFF @13:46:25.90 Gate.power :raw,:OFF @13:46:25.91 Shutdown @13:46:29.07 GoodNews.email "Then, in tube 2: initialPurge; standAloneWCR([nil,100], $da)\n/home/esp/esp2local/mission/standAloneWCR_010224.rb:10\n{2 => 1}\nCTD data unavailable\nCan@13:46:25, 26% flow, 21.3C, 11% humidity, 15.0psia, 13.769V, 0.351A, 6.507Ah, 4.83W",Subject: "Asleep until 13:46" @13:46:30.47 upsync @13:46:30.57
Powering core bus @13:46:33.22 upsync @13:46:36.17
Gate.power :raw,:ON @13:46:37.52 CS.configure :default @13:46:37.57 PS.configure :default @13:46:37.62 SS.configure :default @13:46:37.67 CC.configure :default @13:46:37.71 PC.configure :default @13:46:37.78 Forearm.configure :loose @13:46:37.83 Elbow.configure :loose @13:46:37.90 SC.configure :loose @13:46:37.94 SE.configure :default @13:46:37.99 CH.configure :default @13:46:38.04 PH.configure :default @13:46:38.08 All dwarf controllers running firmware version 3.85 @13:46:38.19 Valve.reset @13:46:38.46 Intake.select :close @13:46:38.52 Exhaust.select :close @13:46:38.53 CTV.dial :air @13:46:38.61 PSV.dial :air @13:46:38.65 CSV.dial :waste1 @13:46:38.82 PS.home.seek 0ml @13:46:38.84
Forearm.home.seek :retract @13:46:39.16 SSV.dial :spit,avoiding: :exhaust @13:46:39.62 Hand.close @13:46:39.64
Elbow.reconfigure :loose @13:46:39.74 Elbow.jog 1500 @13:46:40.03 CS.reconfigure :fast @13:46:40.07 SS.home.seek 0ml @13:46:40.15 CS.home.seek 0ml @13:46:40.29
Elbow.reconfigure :loose @13:46:40.33 Elbow.home.seek :clear @13:46:43.56 SE.home.seek :down @13:46:44.21 SC.reconfigure :tight @13:46:44.26 SC.home.seek :initial @13:46:47.25 PS.stop @13:47:09.47
SC.configure :limp @13:47:09.52 Forearm.seek :retract @13:47:09.59 Elbow.seek :hammer @13:47:10.71 Forearm.reconfigure :tight @13:47:10.76 Forearm.seek retracted @13:47:11.05 PC.home.seek :open @13:47:11.08
CC.home.seek :open @13:47:14.60 Notice.email "At 13:47:14 began in tube 2: initialPurge; standAloneWCR([nil,100], $da)\n/home/esp/esp2local/mission/standAloneWCR_010224.rb:10\n{2 => 1}\nCTD data unavailable\nCan@13:47:14, 26% flow, 21.3C, 10% humidity, 15.0psia, 13.769V, 0.327A, 6.512Ah, 4.50W",Subject: "Started standAloneWCR_010224 phase initialPurge; standAloneWCR([nil,100], $da)" @13:47:14.62
Phase [initialPurge; standAloneWCR([nil,100], $da)] @13:47:14.64 Starting initialPurge @13:47:14.66 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @13:47:14.72 Hand.open @13:47:15.50 Elbow.reconfigure :tight @13:47:15.55 Elbow.seek FlushPuck::Garage @13:47:17.52 Elbow.reconfigure :loose @13:47:17.73 Forearm.reconfigure :loose @13:47:17.83 Forearm.seek :garage @13:47:18.85 Hand.close @13:47:18.91 upsync @13:47:19.64
Forearm.seek :retract @13:47:20.62 Elbow.reconfigure :tight @13:47:20.67 Elbow.seek CC @13:47:23.63 Elbow.reconfigure :loose @13:47:23.73 Forearm.reconfigure :tight @13:47:23.78 Forearm.seek CC @13:47:25.05 CC.seek :closed @13:47:29.67 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.35 @13:47:29.74 Hand.open @13:47:30.44 Forearm.reconfigure :loose @13:47:30.49 Forearm.seek :retract @13:47:31.48 Flushing the Collection Valve::Manifold @13:47:31.60 CSV.dial CSR @13:47:32.99 Pulling 5ml of CSR.flush @13:47:33.00 CSR.select :flush @13:47:33.12 CS.reconfigure :default @13:47:33.16 CS.seek 5ml @13:47:46.96 delay 5 seconds @13:47:51.98 CSR.select :air @13:47:52.55 CS.reconfigure :fast @13:47:52.60 CS.seek 7.5ml @13:47:59.36 CSV.dial :waste @13:48:00.63 CS.reconfigure :default @13:48:00.68 CS.seek 0ml @13:48:20.71 Purging external Intake @13:48:20.82 CSV.dial :flush @13:48:21.27 CS.reconfigure :fast @13:48:21.32 CS.seek 10ml @13:48:45.86 delay 5 seconds @13:48:50.88 CSV.dial CBV @13:48:50.91
CBV.dial CSV @13:48:51.66 CTV.dial :intake @13:48:52.42 Intake.select :open @13:48:52.54 CS.seek 0ml @13:49:16.05 Intake.select :close @13:49:16.15 CTV.dial :air @13:49:16.91 Flushing upper manifold @13:49:17.03 CSV.dial :flush @13:49:17.77 CS.seek 5ml @13:49:30.45 delay 5 seconds @13:49:35.47 CSV.dial :air @13:49:35.96 CS.seek 7.001ml @13:49:41.42 CSV.dial CBV @13:49:41.45
CBV.dial CSV @13:49:42.47 CTV.dial :air @13:49:42.55 CS.seek 0ml @13:49:59.14 delay 7 seconds @13:50:06.16 CS.seek 3ml @13:50:14.23 CSV.dial :waste @13:50:14.69 CS.seek 0ml @13:50:22.59 CSV.dial :flush @13:50:23.04 CS.seek 3ml @13:50:31.11 delay 5 seconds @13:50:36.13 CSV.dial :air @13:50:36.62 CS.seek 4.999ml @13:50:42.39 CSV.dial :air @13:50:42.57 CS.seek 0ml @13:50:54.92 delay 7 seconds @13:51:01.95 CS.seek 3ml @13:51:10.01 CSV.dial :waste @13:51:10.74 CS.seek 0ml @13:51:18.45 Garaging FlushPuck in Collection Clamp @13:51:18.52 Elbow.reconfigure :tight @13:51:18.57 Elbow.seek CC @13:51:18.87 Elbow.reconfigure :loose @13:51:18.96 Forearm.seek CC @13:51:19.91 Hand.close @13:51:20.51 CC.seek :open @13:51:25.49 Forearm.seek :retract @13:51:26.57 Elbow.reconfigure :tight @13:51:26.61 Elbow.seek :garage @13:51:29.53 Elbow.reconfigure :loose @13:51:29.63 Forearm.reconfigure :tight @13:51:29.68 Forearm.seek :garage @13:51:30.77 Hand.open @13:51:31.43 Forearm.reconfigure :loose @13:51:31.48 Forearm.seek :retract @13:51:32.44 Starting stand-alone da WCR @13:51:32.45 da wcr sampling at most 100ml @13:51:32.48 Whole Cell Archive sampling 100ml @13:51:32.50 DAfans.email "Started stand-alone da WCR in tube 2 at 13:51:32UTC05-Jan-24\n{2 => 1}\nCTD data unavailable\nCan@13:47:14, 26% flow, 21.3C, 10% humidity, 15.0psia, 13.769V, 0.327A, 6.512Ah, 4.50W",Subject: "da wcr sampling at most 100ml" @13:51:32.52 Loading wcr puck from tube 2 into Collection Clamp @13:51:32.80 SC.reconfigure :tight @13:51:32.85 SC.seek 2 @13:51:36.58 upsync @13:51:39.55 SC.configure :limp @13:51:39.78 Elbow.reconfigure :tight @13:51:39.83 Elbow.seek 2 @13:51:41.77 Forearm.reconfigure :tight @13:51:41.83 Forearm.seek 2 + 450 counts @13:51:42.88 SE.reconfigure :pick @13:51:42.94 SE.seek :beamBreak @13:51:46.02 SE.configure :default @13:51:46.18 Elbow.seek 2 @13:51:46.38 Forearm.seek 2 + 300 counts @13:51:46.61 Forearm.reconfigure :loose @13:51:46.67 Hand.close @13:51:47.27 SE.seek :down @13:51:47.84 Forearm.seek :retract @13:51:48.85 Elbow.seek CC @13:51:50.04 SE.stop @13:51:50.46 Elbow.reconfigure :loose @13:51:50.57 Forearm.reconfigure :tight @13:51:50.62 Forearm.seek CC @13:51:51.89 CC.seek :closed @13:51:56.43 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.22 @13:51:56.44 Hand.open @13:51:57.06 Forearm.reconfigure :loose @13:51:57.11 Forearm.seek :retract @13:51:58.13 collectSample 100ml w/bubble point of 35psi @13:51:58.14 Priming 7ml @13:51:58.15 Intake.select :close @13:51:58.17 Exhaust.select :close @13:51:58.18 SS.hold @13:51:58.39 Calibrating pressure and temperature sensors @13:51:58.42 CTV.dial :air @13:51:58.43 SSV.dial :spit,avoiding: :exhaust @13:51:58.63 SS.reconfigure :fast @13:51:58.68 SS.seek 5ml @13:52:01.73 SSV.dial CBV @13:52:01.76 CBV.dial SSV @13:52:03.08 delay 3.8 seconds @13:52:07.00 Sensor limits: Intake max 115.4psia; Outlet max 108.2psia @13:52:07.01 SSV.dial :spit @13:52:08.34 SS.seek 0ml @13:52:11.10 SSV.dial CBV @13:52:12.40 CTV.dial :intake @13:52:13.19 SSV.dial CBV @13:52:13.21 CBV.dial SSV @13:52:13.36 Allowing 40s for 7.0ml of remaining 7.0ml @13:52:13.38 Intake.select :open @13:52:15.54 Intake @15.6psia, vacuum from 10.0 to 13.0psi @13:52:15.56 SS.reconfigure :inhaling 2.20ml/s @13:52:15.60 SS.seek 7ml @13:52:22.49 Stroke completed -- waiting for pressure to equalize ... @13:52:22.50 SS.reconfigure :exhale @13:52:22.55 SS.hold @13:52:25.77 Intake.select :close @13:52:25.93 SSV.dial :exhaust @13:52:26.67 Exhaust.select :open @13:52:26.78 SS.seek 0ml @13:52:30.88 Exhaust.select :close @13:52:30.99 Sampling 100.0ml @13:52:31.01 Intake.select :close @13:52:31.02 Exhaust.select :close @13:52:31.03 SS.reconfigure :default @13:52:31.07 SS.hold @13:52:31.28 CTV.dial :intake @13:52:31.45 SSV.dial CBV @13:52:31.48 CBV.dial SSV @13:52:32.26 Allowing 130s for 25.0ml of remaining 100.0ml @13:52:32.28 Intake.select :open @13:52:34.44 Intake @15.6psia, vacuum from 10.0 to 13.0psi @13:52:34.45 SS.reconfigure :inhaling 2.20ml/s @13:52:34.50 SS.seek 25ml @13:52:49.77 Stroke completed -- waiting for pressure to equalize ... @13:52:49.79 SS.reconfigure :exhale @13:52:49.83 SS.hold @13:52:56.33 Intake.select :close @13:52:56.48 SSV.dial :exhaust @13:52:57.65 Exhaust.select :open @13:52:57.77 SS.seek 0ml @13:53:06.68 Exhaust.select :close @13:53:06.79 SSV.dial CBV @13:53:06.82 CBV.dial SSV @13:53:07.59 Allowing 130s for 25.0ml of remaining 75.0ml @13:53:07.60 Intake.select :open @13:53:09.77 Intake @15.8psia, vacuum from 10.0 to 13.0psi @13:53:09.79 SS.reconfigure :inhaling 2.20ml/s @13:53:09.84 SS.seek 25ml @13:53:23.36 Vacuum too high -- waiting up to 40 seconds @13:53:23.38 SS.reconfigure :exhale @13:53:23.43 SS.hold @13:53:24.05 Intake @12.6psia, vacuum from 7.1 to 10.1psi @13:53:24.07 SS.reconfigure :inhaling 1.76ml/s @13:53:24.12 SS.seek 25ml @13:53:26.82 Stroke completed -- waiting for pressure to equalize ... @13:53:26.83 SS.reconfigure :exhale @13:53:26.88 SS.hold @13:53:33.42 Intake.select :close @13:53:33.59 SSV.dial :exhaust @13:53:34.34 Exhaust.select :open @13:53:34.45 SS.seek 0ml @13:53:43.34 Exhaust.select :close @13:53:43.45 SSV.dial CBV @13:53:43.48 CBV.dial SSV @13:53:44.21 Allowing 130s for 25.0ml of remaining 50.0ml @13:53:44.23 Intake.select :open @13:53:46.39 Intake @15.8psia, vacuum from 10.0 to 13.0psi @13:53:46.41 SS.reconfigure :inhaling 1.76ml/s @13:53:46.46 SS.seek 25ml @13:54:03.02 Vacuum too high -- waiting up to 40 seconds @13:54:03.03 SS.reconfigure :exhale @13:54:03.08 SS.hold @13:54:03.93 Intake @12.8psia, vacuum from 7.3 to 10.3psi @13:54:03.95 SS.reconfigure :inhaling 1.41ml/s @13:54:03.99 SS.seek 25ml @13:54:05.99 Stroke completed -- waiting for pressure to equalize ... @13:54:06.00 SS.reconfigure :exhale @13:54:06.05 SS.hold @13:54:11.46 Intake.select :close @13:54:11.62 SSV.dial :exhaust @13:54:12.37 Exhaust.select :open @13:54:12.48 SS.seek 0ml @13:54:21.34 Exhaust.select :close @13:54:21.45 SSV.dial CBV @13:54:21.46 CBV.dial SSV @13:54:22.26 Allowing 130s for 25.0ml of remaining 25.0ml @13:54:22.27 Intake.select :open @13:54:24.44 Intake @15.8psia, vacuum from 10.0 to 13.0psi @13:54:24.46 SS.reconfigure :inhaling 1.41ml/s @13:54:24.50 SS.seek 25ml @13:54:45.46 Stroke completed -- waiting for pressure to equalize ... @13:54:45.47 SS.reconfigure :exhale @13:54:45.52 SS.hold @13:54:52.44 Intake.select :close @13:54:52.59 SSV.dial :exhaust @13:54:53.34 Exhaust.select :open @13:54:53.45 SS.seek 0ml @13:55:02.33 Exhaust.select :close @13:55:02.44 Sampled 100.0ml @13:55:02.46 Drying puck before fixing for 5:00 @13:55:02.47 CBV.dial :waste2b @13:55:03.21 delay 1:00 @13:56:03.24 CSV.dial :air @13:56:04.09 CS.seek 10ml @13:56:28.79 Pumping wcr puck from tube 2 up to 35psi... @13:56:28.81 CBV.dial SSV @13:56:30.06 CBV.dial between CSV and waste2b @13:56:31.01 CSV.dial between air and CTV @13:56:31.34 CS.seek 7.355ml @13:56:38.17 CTV.dial CSV,avoiding: :air @13:56:38.19 CSV.dial CTV @13:56:39.00 CS.reconfigure :pressurize @13:56:39.05 CS.seek 0ml @13:56:57.62 CBV.dial :waste2b @13:56:57.95 CS.reconfigure :default @13:56:58.00 CS.seek 0ml @13:56:59.70 delay 1:00 @13:57:59.72 CTV.dial :air,avoiding: :intake @13:58:01.01 Fix #1 of 2 (with RNAlater) for 2:30 @13:58:01.03 CSV.dial CSR @13:58:01.46 Pulling 2ml of CSR.RNAlater @13:58:01.47 CSR.select :RNAlater @13:58:01.59 CS.seek 2ml @13:58:07.66 delay 5 seconds @13:58:12.68 CSR.select :air @13:58:12.80 CS.reconfigure :fast @13:58:12.85 CS.seek 10ml @13:58:32.51 Pumping wcr puck from tube 2 up to 35psi... @13:58:32.53 CBV.dial SSV @13:58:33.76 CBV.dial between CSV and waste2b @13:58:34.72 CSV.dial between air and CTV @13:58:35.33 CS.seek 7.304ml @13:58:42.30 CTV.dial CSV,avoiding: :air @13:58:42.31 CSV.dial CTV @13:58:44.05 CS.reconfigure :pressurize @13:58:44.16 CS.seek 0ml @13:59:02.94 delay 2:30 @14:01:32.96 CBV.dial :waste2b @14:01:33.29 CS.reconfigure :default @14:01:33.34 CS.seek 0ml @14:01:34.60 delay 1:00 @14:02:34.63 CTV.dial :air,avoiding: :intake @14:02:35.91 Fix #2 of 2 (with RNAlater) for 2:30 @14:02:35.93 CSV.dial CSR @14:02:36.36 Pulling 2ml of CSR.RNAlater @14:02:36.37 CSR.select :RNAlater @14:02:36.49 CS.seek 2ml @14:02:42.41 delay 5 seconds @14:02:47.43 CSR.select :air @14:02:47.55 CS.reconfigure :fast @14:02:47.60 CS.seek 10ml @14:03:07.35 Pumping wcr puck from tube 2 up to 35psi... @14:03:07.37 CBV.dial SSV @14:03:08.60 CBV.dial between CSV and waste2b @14:03:09.56 CSV.dial between air and CTV @14:03:10.17 CS.seek 7.27ml @14:03:17.23 CTV.dial CSV,avoiding: :air @14:03:17.25 CSV.dial CTV @14:03:18.62 CS.reconfigure :pressurize @14:03:18.67 CS.seek 0ml @14:03:34.28 delay 2:30 @14:06:04.30 CBV.dial :waste2b @14:06:04.63 CS.reconfigure :default @14:06:04.68 CS.seek 0ml @14:06:09.12 delay 1:00 @14:07:09.15 CTV.dial :air,avoiding: :intake @14:07:10.51 Storing wcr puck in Collection Clamp to tube 1 @14:07:10.69 SC.reconfigure :tight @14:07:10.73 SC.seek 1 @14:07:14.32 SC.configure :limp @14:07:14.53 Elbow.reconfigure :tight @14:07:14.58 Elbow.seek 1 @14:07:16.37 Forearm.reconfigure :tight @14:07:16.42 Forearm.seek 1 + 450 counts @14:07:17.53 SE.reconfigure :pick @14:07:17.59 SE.seek :beamBreak @14:07:20.43 SE.configure :default @14:07:20.92 SE.seek :place @14:07:21.51 Forearm.reconfigure :loose @14:07:21.56 Forearm.seek :retract @14:07:22.51 Elbow.seek CC @14:07:24.19 Elbow.reconfigure :loose @14:07:24.29 Forearm.seek CC @14:07:25.28 Hand.close @14:07:25.87 CC.seek :open @14:07:30.60 Forearm.seek :retract @14:07:31.60 Elbow.reconfigure :tight @14:07:31.65 Elbow.seek 1 @14:07:33.44 Elbow.reconfigure :limp @14:07:33.50 Forearm.seek 1 @14:07:34.49 Elbow.hold @14:07:34.56 Elbow.reconfigure :tight @14:07:34.61 Hand.open @14:07:35.35 Forearm.force -20 @14:07:35.40 Forearm.seek 1 + 318 counts @14:07:35.68 Forearm.reconfigure :tight @14:07:35.73 Forearm.seek 1 + 450 counts @14:07:35.99 SE.seek :down @14:07:37.12 Forearm.reconfigure :loose @14:07:37.17 Forearm.seek :retract @14:07:38.70 SE.stop @14:07:38.74
Starting finalPurge @14:07:38.75 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:07:38.95 Elbow.seek FlushPuck::Garage @14:07:40.73 Elbow.reconfigure :loose @14:07:41.20 Forearm.seek :garage @14:07:42.28 Hand.close @14:07:42.98 Forearm.seek :retract @14:07:43.96 Elbow.reconfigure :tight @14:07:44.01 Elbow.seek CC @14:07:46.92 Elbow.reconfigure :loose @14:07:47.02 Forearm.reconfigure :tight @14:07:47.07 Forearm.seek CC @14:07:48.37 CC.seek :closed @14:07:52.89 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.40 @14:07:52.96 Hand.open @14:07:53.67 Forearm.reconfigure :loose @14:07:53.72 Forearm.seek :retract @14:07:54.66 Flushing the Collection Valve::Manifold @14:07:54.78 CSV.dial CSR @14:07:55.32 Pulling 5ml of CSR.flush @14:07:55.33 CSR.select :flush @14:07:55.45 CS.seek 5ml @14:08:09.28 delay 5 seconds @14:08:14.30 CSR.select :air @14:08:14.46 CS.reconfigure :fast @14:08:14.51 CS.seek 7.5ml @14:08:21.31 CSV.dial :waste @14:08:22.58 CS.reconfigure :default @14:08:22.63 CS.seek 0ml @14:08:42.62 Flushing lower manifold's waste2b port @14:08:42.74 CSV.dial :flush @14:08:43.19 CS.reconfigure :fast @14:08:43.24 CS.seek 5ml @14:08:56.03 delay 5 seconds @14:09:01.05 CSV.dial :air @14:09:01.54 CS.seek 7ml @14:09:07.15 CSV.dial CTV @14:09:07.18
CTV.dial CSV @14:09:08.56 CBV.dial :waste2b @14:09:09.09 CS.seek 0ml @14:09:26.00 Disinfecting external Intake with kill @14:09:26.12 CSV.dial CSR @14:09:26.58 Pulling 8ml of CSR.kill CSR.select :kill @14:09:26.70 CS.seek 8ml @14:09:46.68 delay 5 seconds @14:09:51.70 CSR.select :air @14:09:51.82 CS.seek 10ml @14:09:57.34 CSV.dial CBV @14:09:57.36
CBV.dial CSV @14:09:58.42 CTV.dial :intake @14:09:59.20 Intake.select :open @14:09:59.31 CS.seek 2ml @14:10:18.40 Intake.select :close @14:10:18.50 CTV.dial :air @14:10:19.25 CS.reconfigure :default @14:10:19.30 CS.seek 0ml @14:10:25.40 CSV.dial CSR @14:10:26.42 Pulling 5ml of CSR.flush CSR.select :flush @14:10:26.54 CS.seek 5ml @14:10:40.31 delay 5 seconds @14:10:45.33 CSR.select :air @14:10:45.44 CS.seek 10ml @14:10:59.09 CSV.dial CBV @14:11:00.09 CS.seek 0ml @14:11:26.65 Garaging FlushPuck in Collection Clamp @14:11:26.73 Elbow.reconfigure :tight @14:11:26.77 Elbow.seek CC @14:11:27.03 Elbow.reconfigure :loose @14:11:27.12 Forearm.seek CC @14:11:28.12 Hand.close @14:11:28.74 CC.seek :open @14:11:33.39 Forearm.seek :retract @14:11:34.42 Elbow.reconfigure :tight @14:11:34.47 Elbow.seek :garage @14:11:37.38 Elbow.reconfigure :loose @14:11:37.48 Forearm.reconfigure :tight @14:11:37.53 Forearm.seek :garage @14:11:38.56 Hand.open @14:11:39.17 Forearm.reconfigure :loose @14:11:39.21 Forearm.seek :retract @14:11:40.20 Completed [initialPurge; standAloneWCR([nil,100], $da)] begun 13:47:14 @14:11:40.25 Asleep after completing standAloneWCR_010224 assays @14:11:40.27 GoodNews.email "{1 => 1,\n 2 => 0}\nCTD data unavailable\nCan@14:06:28, 26% flow, 22.6C, 9% humidity, 15.0psia, 13.710V, 0.502A, 6.674Ah, 6.88W\nSampled 100.0ml",Subject: "Asleep after completing standAloneWCR_010224 assays" @14:11:44.73 upsync + ESPwhich standAloneWCR_010224.rb + fn=standAloneWCR_010224.rb + IFS=: + set /home/esp/esp2/mission . + fullPath=/home/esp/esp2/mission/standAloneWCR_010224.rb + [ -f /home/esp/esp2/mission/standAloneWCR_010224.rb ] + realpath /home/esp/esp2/mission/standAloneWCR_010224.rb + return 0 + scriptPath=/home/esp/esp2local/mission/standAloneWCR_010224.rb + exec ruby /home/esp/esp2/lib/esp.rb /home/esp/esp2local/mission/standAloneWCR_010224.rb @14:28:04.70UTC05-Jan-24
ESP2 v3.42 I2C Gateway v3.20 for core bus @14:28:07.42 Configuration for short puck jake 1/11/21 by brent@mbari.org /home/esp/esp2/type/shallow/jake/configure.rb @14:28:11.07 Polling Can every 10:00 @14:28:11.09 Cleanly exited at 14:27:42UTC05-Jan-24 @14:28:11.13 Loading /home/esp/esp2local/mission/standAloneWCR_010224.rb mission @14:28:11.20 Preparing to sleep until + 00:00:05 @14:28:11.21 GoodNews.email "Starting standAloneWCR_010224 w/pucks in tube 2\n{2 => 1}\nCTD data unavailable\nCan@14:28:11, 25% flow, 23.1C, 9% humidity, 15.0psia, 13.739V, 0.339A, 6.805Ah, 4.65W",Subject: "standAloneWCR_010224 begun" @14:28:11.25
Asleep til + 00:00:05 @14:28:11.29 Continuing at 14:28 @14:28:11.54 Gate.power :core,:OFF @14:28:11.55 Gate.power :raw,:OFF @14:28:11.56 Shutdown @14:28:14.11 GoodNews.email "Then, in tube 2: initialPurge; standAloneWCR([nil,100], $da)\n/home/esp/esp2local/mission/standAloneWCR_010224.rb:10\n{2 => 1}\nCTD data unavailable\nCan@14:28:11, 26% flow, 23.1C, 9% humidity, 15.0psia, 13.744V, 0.333A, 6.805Ah, 4.57W",Subject: "Asleep until 14:28" @14:28:15.45 upsync @14:28:16.17
Powering core bus @14:28:18.23 upsync @14:28:21.38
Gate.power :raw,:ON @14:28:22.75 CS.configure :default @14:28:22.80 PS.configure :default @14:28:22.85 SS.configure :default @14:28:22.89 CC.configure :default @14:28:22.94 PC.configure :default @14:28:23.01 Forearm.configure :loose @14:28:23.05 Elbow.configure :loose @14:28:23.12 SC.configure :loose @14:28:23.17 SE.configure :default @14:28:23.22 CH.configure :default @14:28:23.25 PH.configure :default @14:28:23.29 All dwarf controllers running firmware version 3.85 @14:28:23.40 Valve.reset @14:28:23.75 Intake.select :close @14:28:23.79 PSV.dial :air @14:28:23.83 Exhaust.select :close @14:28:23.87 CTV.dial :air @14:28:23.99 CSV.dial :waste1 @14:28:24.02 PS.home.seek 0ml @14:28:24.05
Forearm.home.seek :retract @14:28:24.52 SSV.dial :spit,avoiding: :exhaust @14:28:24.83 Hand.close @14:28:24.88
Elbow.reconfigure :loose @14:28:24.95 Elbow.jog 1500 @14:28:25.36 Elbow.reconfigure :loose @14:28:25.42 Elbow.home.seek :clear @14:28:25.46 CS.reconfigure :fast @14:28:25.52 SS.home.seek 0ml @14:28:25.61 CS.home.seek 0ml @14:28:28.69
SE.home.seek :down @14:28:29.30 SC.reconfigure :tight @14:28:29.35 SC.home.seek :initial @14:28:32.33 PS.stop @14:28:54.54
SC.configure :limp @14:28:54.60 Forearm.seek :retract @14:28:54.66 Elbow.seek :hammer @14:28:55.66 Forearm.reconfigure :tight @14:28:55.71 Forearm.seek retracted @14:28:55.97 PC.home.seek :open @14:28:56.00
CC.home.seek :open @14:28:59.44 Phase [initialPurge; standAloneWCR([nil,100], $da)] @14:28:59.46 Notice.email "At 14:28:59 began in tube 2: initialPurge; standAloneWCR([nil,100], $da)\n/home/esp/esp2local/mission/standAloneWCR_010224.rb:10\n{2 => 1}\nCTD data unavailable\nCan@14:28:59, 26% flow, 23.0C, 9% humidity, 15.0psia, 13.754V, 0.296A, 6.810Ah, 4.08W",Subject: "Started standAloneWCR_010224 phase initialPurge; standAloneWCR([nil,100], $da)" @14:28:59.48
Starting initialPurge @14:28:59.50 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:28:59.58 Hand.open @14:29:00.38 Elbow.reconfigure :tight @14:29:00.42 Elbow.seek FlushPuck::Garage @14:29:02.46 Elbow.reconfigure :loose @14:29:02.67 Forearm.reconfigure :loose @14:29:02.78 Forearm.seek :garage @14:29:03.73 Hand.close @14:29:03.85 upsync @14:29:04.49
Forearm.seek :retract @14:29:05.49 Elbow.reconfigure :tight @14:29:05.54 Elbow.seek CC @14:29:08.43 Elbow.reconfigure :loose @14:29:08.54 Forearm.reconfigure :tight @14:29:08.58 Forearm.seek CC @14:29:09.87 CC.seek :closed @14:29:14.48 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.34 @14:29:14.54 Hand.open @14:29:15.17 Forearm.reconfigure :loose @14:29:15.22 Forearm.seek :retract @14:29:16.24 Flushing the Collection Valve::Manifold @14:29:16.36 CSV.dial CSR @14:29:17.63 Pulling 5ml of CSR.flush CSR.select :flush @14:29:17.75 CS.reconfigure :default @14:29:17.79 CS.seek 5ml @14:29:31.59 delay 5 seconds @14:29:36.61 CSR.select :air @14:29:37.19 CS.reconfigure :fast @14:29:37.24 CS.seek 7.501ml @14:29:44.00 CSV.dial :waste @14:29:45.27 CS.reconfigure :default @14:29:45.32 CS.seek 0ml @14:30:05.47 Purging external Intake @14:30:05.59 CSV.dial :flush @14:30:06.04 CS.reconfigure :fast @14:30:06.08 CS.seek 10ml @14:30:30.53 delay 5 seconds @14:30:35.55 CSV.dial CBV @14:30:35.58
CBV.dial CSV @14:30:36.34 CTV.dial :intake @14:30:37.12 Intake.select :open @14:30:37.24 CS.seek 0ml @14:31:00.78 Intake.select :close @14:31:00.88 CTV.dial :air @14:31:01.64 Flushing upper manifold @14:31:01.76 CSV.dial :flush @14:31:02.51 CS.seek 5ml @14:31:15.34 delay 5 seconds @14:31:20.36 CSV.dial :air @14:31:20.86 CS.seek 7.001ml @14:31:26.59 CSV.dial CBV @14:31:26.62
CBV.dial CSV @14:31:27.65 CTV.dial :air @14:31:27.74 CS.seek 0ml @14:31:44.22 delay 7 seconds @14:31:51.24 CS.seek 3ml @14:31:59.28 CSV.dial :waste @14:31:59.74 CS.seek 0ml @14:32:07.67 CSV.dial :flush @14:32:08.11 CS.seek 3ml @14:32:16.22 delay 5 seconds @14:32:21.23 CSV.dial :air @14:32:21.73 CS.seek 5ml @14:32:27.43 CSV.dial :air @14:32:27.52 CS.seek 0ml @14:32:39.65 delay 7 seconds @14:32:46.68 CS.seek 3ml @14:32:54.68 CSV.dial :waste @14:32:55.42 CS.seek 0ml @14:33:03.09 Garaging FlushPuck in Collection Clamp @14:33:03.16 Elbow.reconfigure :tight @14:33:03.21 Elbow.seek CC @14:33:03.44 Elbow.reconfigure :loose @14:33:03.54 Forearm.seek CC @14:33:04.59 Hand.close @14:33:05.24 CC.seek :open @14:33:10.30 Forearm.seek :retract @14:33:11.33 Elbow.reconfigure :tight @14:33:11.38 Elbow.seek :garage @14:33:14.35 Elbow.reconfigure :loose @14:33:14.45 Forearm.reconfigure :tight @14:33:14.50 Forearm.seek :garage @14:33:15.48 Hand.open @14:33:16.17 Forearm.reconfigure :loose @14:33:16.21 Forearm.seek :retract @14:33:17.20 Starting stand-alone da WCR @14:33:17.21 da wcr sampling at most 100ml @14:33:17.25 Whole Cell Archive sampling 100ml @14:33:17.26 DAfans.email "Started stand-alone da WCR in tube 2 at 14:33:17UTC05-Jan-24\n{2 => 1}\nCTD data unavailable\nCan@14:28:59, 26% flow, 23.0C, 9% humidity, 15.0psia, 13.754V, 0.296A, 6.810Ah, 4.08W",Subject: "da wcr sampling at most 100ml" @14:33:17.28 Loading wcr puck from tube 2 into Collection Clamp @14:33:17.56 SC.reconfigure :tight @14:33:17.61 SC.seek 2 @14:33:21.25 upsync @14:33:24.39 SC.configure :limp @14:33:24.62 Elbow.reconfigure :tight @14:33:24.68 Elbow.seek 2 @14:33:26.63 Forearm.reconfigure :tight @14:33:26.69 Forearm.seek 2 + 450 counts @14:33:27.72 SE.reconfigure :pick @14:33:27.78 SE.seek :beamBreak @14:33:30.86 SE.configure :default @14:33:31.03 Elbow.seek 2 @14:33:31.22 Forearm.seek 2 + 300 counts @14:33:31.42 Forearm.reconfigure :loose @14:33:31.47 Hand.close @14:33:32.13 SE.seek :down @14:33:32.76 Forearm.seek :retract @14:33:33.70 Elbow.seek CC @14:33:34.91 SE.stop @14:33:35.37 Elbow.reconfigure :loose @14:33:35.47 Forearm.reconfigure :tight @14:33:35.52 Forearm.seek CC @14:33:36.79 CC.seek :closed @14:33:41.37 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.17 @14:33:41.39 Hand.open @14:33:42.04 Forearm.reconfigure :loose @14:33:42.09 Forearm.seek :retract @14:33:43.08 collectSample 100ml w/bubble point of 35psi @14:33:43.09 Priming 7ml @14:33:43.10 Intake.select :close @14:33:43.12 Exhaust.select :close @14:33:43.13 SS.hold @14:33:43.33 Calibrating pressure and temperature sensors @14:33:43.36 CTV.dial :air @14:33:43.38 SSV.dial :spit,avoiding: :exhaust @14:33:43.47 SS.reconfigure :fast @14:33:43.52 SS.seek 5ml @14:33:46.37 SSV.dial CBV @14:33:46.39 CBV.dial SSV @14:33:47.76 delay 3.8 seconds @14:33:51.69 Sensor limits: Intake max 115.1psia; Outlet max 108.4psia @14:33:51.70 SSV.dial :spit @14:33:53.01 SS.seek 0ml @14:33:55.98 SSV.dial CBV @14:33:57.29 CTV.dial :intake @14:33:58.07 SSV.dial CBV @14:33:58.09 CBV.dial SSV @14:33:58.24 Allowing 40s for 7.0ml of remaining 7.0ml @14:33:58.25 Intake.select :open @14:34:00.42 Intake @15.5psia, vacuum from 10.0 to 13.0psi @14:34:00.43 SS.reconfigure :inhaling 2.20ml/s @14:34:00.48 SS.seek 7ml @14:34:07.36 Stroke completed -- waiting for pressure to equalize ... @14:34:07.38 SS.reconfigure :exhale @14:34:07.42 SS.hold @14:34:11.14 Intake.select :close @14:34:11.29 SSV.dial :exhaust @14:34:12.04 Exhaust.select :open @14:34:12.16 SS.seek 0ml @14:34:16.37 Exhaust.select :close @14:34:16.47 Sampling 100.0ml @14:34:16.49 Intake.select :close @14:34:16.50 Exhaust.select :close @14:34:16.51 SS.reconfigure :default @14:34:16.55 SS.hold @14:34:16.76 CTV.dial :intake @14:34:16.84 SSV.dial CBV @14:34:16.86 CBV.dial SSV @14:34:17.62 Allowing 130s for 25.0ml of remaining 100.0ml @14:34:17.63 Intake.select :open @14:34:19.80 Intake @15.8psia, vacuum from 10.0 to 13.0psi @14:34:19.81 SS.reconfigure :inhaling 2.20ml/s @14:34:19.86 SS.seek 25ml @14:34:35.02 Stroke completed -- waiting for pressure to equalize ... @14:34:35.03 SS.reconfigure :exhale @14:34:35.08 SS.hold @14:34:40.70 Intake.select :close @14:34:40.86 SSV.dial :exhaust @14:34:42.04 Exhaust.select :open @14:34:42.16 SS.seek 0ml @14:34:51.04 Exhaust.select :close @14:34:51.15 SSV.dial CBV @14:34:51.17 CBV.dial SSV @14:34:51.93 Allowing 130s for 25.0ml of remaining 75.0ml @14:34:51.94 Intake.select :open @14:34:54.11 Intake @15.8psia, vacuum from 10.0 to 13.0psi @14:34:54.13 SS.reconfigure :inhaling 2.20ml/s @14:34:54.18 SS.seek 25ml @14:35:09.33 Stroke completed -- waiting for pressure to equalize ... @14:35:09.35 SS.reconfigure :exhale @14:35:09.39 SS.hold @14:35:15.88 Intake.select :close @14:35:16.04 SSV.dial :exhaust @14:35:16.79 Exhaust.select :open @14:35:16.90 SS.seek 0ml @14:35:25.82 Exhaust.select :close @14:35:25.93 SSV.dial CBV @14:35:25.95 CBV.dial SSV @14:35:26.71 Allowing 130s for 25.0ml of remaining 50.0ml @14:35:26.72 Intake.select :open @14:35:28.89 Intake @15.8psia, vacuum from 10.0 to 13.0psi @14:35:28.91 SS.reconfigure :inhaling 2.20ml/s @14:35:28.95 SS.seek 25ml @14:35:42.18 Vacuum too high -- waiting up to 40 seconds @14:35:42.20 SS.reconfigure :exhale @14:35:42.25 SS.hold @14:35:42.96 Intake @12.8psia, vacuum from 7.3 to 10.3psi @14:35:42.97 SS.reconfigure :inhaling 1.76ml/s @14:35:43.03 SS.seek 25ml @14:35:46.14 Stroke completed -- waiting for pressure to equalize ... @14:35:46.16 SS.reconfigure :exhale @14:35:46.20 SS.hold @14:35:52.14 Intake.select :close @14:35:52.30 SSV.dial :exhaust @14:35:53.07 Exhaust.select :open @14:35:53.19 SS.seek 0ml @14:36:02.13 Exhaust.select :close @14:36:02.24 SSV.dial CBV @14:36:02.26 CBV.dial SSV @14:36:03.03 Allowing 130s for 25.0ml of remaining 25.0ml @14:36:03.05 Intake.select :open @14:36:05.21 Intake @15.9psia, vacuum from 10.0 to 13.0psi @14:36:05.23 SS.reconfigure :inhaling 1.76ml/s @14:36:05.28 SS.seek 25ml @14:36:19.03 Vacuum too high -- waiting up to 40 seconds @14:36:19.04 SS.reconfigure :exhale @14:36:19.10 SS.hold @14:36:20.56 Intake @13.4psia, vacuum from 7.9 to 10.9psi @14:36:20.58 SS.reconfigure :inhaling 1.41ml/s @14:36:20.63 SS.seek 25ml @14:36:26.21 Stroke completed -- waiting for pressure to equalize ... @14:36:26.22 SS.reconfigure :exhale @14:36:26.26 SS.hold @14:36:32.05 Intake.select :close @14:36:32.21 SSV.dial :exhaust @14:36:32.96 Exhaust.select :open @14:36:33.07 SS.seek 0ml @14:36:42.00 Exhaust.select :close @14:36:42.11 Sampled 100.0ml @14:36:42.13 Drying puck before fixing for 5:00 @14:36:42.14 CBV.dial :waste2b @14:36:42.87 delay 1:00 @14:37:42.89 CSV.dial :air @14:37:43.64 CS.seek 10ml @14:38:08.12 Pumping wcr puck from tube 2 up to 35psi... @14:38:08.14 CBV.dial SSV @14:38:09.40 CBV.dial between CSV and waste2b @14:38:10.36 CSV.dial between air and CTV @14:38:10.79 CS.seek 7.37ml @14:38:17.56 CTV.dial CSV,avoiding: :air @14:38:17.58 CSV.dial CTV @14:38:18.37 CS.reconfigure :pressurize @14:38:18.42 CS.seek 0ml @14:38:33.48 CBV.dial :waste2b @14:38:33.81 CS.reconfigure :default @14:38:33.86 CS.seek 0ml @14:38:38.83 delay 1:00 @14:39:38.86 CTV.dial :air,avoiding: :intake @14:39:40.18 Fix #1 of 2 (with RNAlater) for 2:30 @14:39:40.20 CSV.dial CSR @14:39:40.63 Pulling 2ml of CSR.RNAlater @14:39:40.64 CSR.select :RNAlater @14:39:40.76 CS.seek 2ml @14:39:46.68 delay 5 seconds @14:39:51.70 CSR.select :air @14:39:51.81 CS.reconfigure :fast @14:39:51.86 CS.seek 10ml @14:40:11.46 Pumping wcr puck from tube 2 up to 35psi... @14:40:11.48 CBV.dial SSV @14:40:12.71 CBV.dial between CSV and waste2b @14:40:13.67 CSV.dial between air and CTV @14:40:14.28 CS.seek 7.327ml @14:40:21.59 CTV.dial CSV,avoiding: :air @14:40:21.61 CSV.dial CTV @14:40:23.13 CS.reconfigure :pressurize @14:40:23.19 CS.seek 0ml @14:40:36.42 delay 2:30 @14:43:06.44 CBV.dial :waste2b @14:43:06.77 CS.reconfigure :default @14:43:06.82 CS.seek 0ml @14:43:13.77 delay 1:00 @14:44:13.80 CTV.dial :air,avoiding: :intake @14:44:15.15 Fix #2 of 2 (with RNAlater) for 2:30 @14:44:15.16 CSV.dial CSR @14:44:15.59 Pulling 2ml of CSR.RNAlater @14:44:15.60 CSR.select :RNAlater @14:44:15.72 CS.seek 2ml @14:44:21.89 delay 5 seconds @14:44:26.91 CSR.select :air @14:44:27.03 CS.reconfigure :fast @14:44:27.08 CS.seek 10ml @14:44:46.86 Pumping wcr puck from tube 2 up to 35psi... @14:44:46.88 CBV.dial SSV @14:44:48.13 CBV.dial between CSV and waste2b @14:44:49.07 CSV.dial between air and CTV @14:44:49.68 CS.seek 7.31ml @14:44:56.65 CTV.dial CSV,avoiding: :air @14:44:56.68 CSV.dial CTV @14:44:58.02 CS.reconfigure :pressurize @14:44:58.07 CS.seek 0ml @14:45:17.26 CSV.dial :air @14:45:17.72 CS.reconfigure :fast @14:45:17.77 CS.seek 10ml @14:45:42.38 CSV.dial between air and CTV @14:45:42.71 CS.seek 4.131ml @14:45:56.90 CTV.dial CSV,avoiding: :air @14:45:56.92 CSV.dial CTV @14:45:57.28 CS.reconfigure :pressurize @14:45:57.34 CS.seek 0ml @14:46:06.82 delay 2:30 @14:48:36.84 CBV.dial :waste2b @14:48:37.15 CS.reconfigure :default @14:48:37.21 CS.seek 0ml @14:48:39.48 delay 1:00 @14:49:39.51 CTV.dial :air,avoiding: :intake @14:49:40.88 Storing wcr puck in Collection Clamp to tube 1 @14:49:41.49 SC.reconfigure :tight @14:49:41.55 SC.seek 1 @14:49:45.12 SC.configure :limp @14:49:45.35 Elbow.reconfigure :tight @14:49:45.40 Elbow.seek 1 @14:49:47.15 Forearm.reconfigure :tight @14:49:47.20 Forearm.seek 1 + 450 counts @14:49:48.24 SE.reconfigure :pick @14:49:48.30 SE.seek :beamBreak @14:49:51.14 SE.configure :default @14:49:51.20 SE.seek :place @14:49:51.76 Forearm.reconfigure :loose @14:49:51.81 Forearm.seek :retract @14:49:52.77 Elbow.seek CC @14:49:54.43 Elbow.reconfigure :loose @14:49:54.53 Forearm.seek CC @14:49:55.48 Hand.close @14:49:56.10 CC.seek :open @14:50:00.86 Forearm.seek :retract @14:50:01.85 Elbow.reconfigure :tight @14:50:01.90 Elbow.seek 1 @14:50:03.64 Elbow.reconfigure :limp @14:50:03.69 Forearm.seek 1 @14:50:04.70 Elbow.hold @14:50:04.77 Elbow.reconfigure :tight @14:50:04.82 Hand.open @14:50:05.59 Forearm.force -20 @14:50:05.63 Forearm.seek 1 + 314 counts @14:50:06.36 Forearm.reconfigure :tight @14:50:06.48 Forearm.seek 1 + 450 counts @14:50:06.76 SE.seek :down @14:50:07.89 Forearm.reconfigure :loose @14:50:07.94 Forearm.seek :retract @14:50:09.43 SE.stop @14:50:09.47
Starting finalPurge @14:50:09.48 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:50:09.68 Elbow.seek FlushPuck::Garage @14:50:11.53 Elbow.reconfigure :loose @14:50:11.63 Forearm.seek :garage @14:50:12.64 Hand.close @14:50:13.34 Forearm.seek :retract @14:50:14.33 Elbow.reconfigure :tight @14:50:14.38 Elbow.seek CC @14:50:17.29 Elbow.reconfigure :loose @14:50:17.39 Forearm.reconfigure :tight @14:50:17.43 Forearm.seek CC @14:50:18.73 CC.seek :closed @14:50:23.27 Collection Clamp onSpring/offSpring for 14/27 samples, on/off torque 2.23 @14:50:23.34 Hand.open @14:50:24.03 Forearm.reconfigure :loose @14:50:24.08 Forearm.seek :retract @14:50:25.05 Flushing the Collection Valve::Manifold @14:50:25.17 CSV.dial CSR @14:50:25.60 Pulling 5ml of CSR.flush @14:50:25.61 CSR.select :flush @14:50:25.73 CS.seek 5ml @14:50:39.38 delay 5 seconds @14:50:44.40 CSR.select :air @14:50:45.03 CS.reconfigure :fast @14:50:45.07 CS.seek 7.5ml @14:50:51.88 CSV.dial :waste @14:50:53.15 CS.reconfigure :default @14:50:53.20 CS.seek 0ml @14:51:13.23 Flushing lower manifold's waste2b port @14:51:13.36 CSV.dial :flush @14:51:13.79 CS.reconfigure :fast @14:51:13.84 CS.seek 5ml @14:51:26.76 delay 5 seconds @14:51:31.78 CSV.dial :air @14:51:32.29 CS.seek 7ml @14:51:37.86 CSV.dial CTV @14:51:37.87
CTV.dial CSV @14:51:39.23 CBV.dial :waste2b @14:51:39.24 CS.seek 0ml @14:51:56.13 Disinfecting external Intake with kill @14:51:56.25 CSV.dial CSR @14:51:56.68 Pulling 8ml of CSR.kill @14:51:56.69 CSR.select :kill @14:51:56.81 CS.seek 8ml @14:52:16.66 delay 5 seconds @14:52:21.68 CSR.select :air @14:52:21.80 CS.seek 10ml @14:52:27.38 CSV.dial CBV @14:52:27.40
CBV.dial CSV @14:52:28.46 CTV.dial :intake @14:52:29.22 Intake.select :open @14:52:29.34 CS.seek 2ml @14:52:48.19 Intake.select :close @14:52:48.30 CTV.dial :air @14:52:49.04 CS.reconfigure :default @14:52:49.09 CS.seek 0ml @14:52:55.04 CSV.dial CSR @14:52:56.04 Pulling 5ml of CSR.flush @14:52:56.05 CSR.select :flush @14:52:56.16 CS.seek 5ml @14:53:09.85 delay 5 seconds @14:53:14.87 CSR.select :air @14:53:14.99 CS.seek 10ml @14:53:28.60 CSV.dial CBV @14:53:29.60 CS.seek 0ml @14:53:56.01 Garaging FlushPuck in Collection Clamp @14:53:56.08 Elbow.reconfigure :tight @14:53:56.12 Elbow.seek CC @14:53:56.35 Elbow.reconfigure :loose @14:53:56.45 Forearm.seek CC @14:53:57.39 Hand.close @14:53:57.97 CC.seek :open @14:54:02.62 Forearm.seek :retract @14:54:04.04 Elbow.reconfigure :tight @14:54:04.10 Elbow.seek :garage @14:54:07.05 Elbow.reconfigure :loose @14:54:07.15 Forearm.reconfigure :tight @14:54:07.20 Forearm.seek :garage @14:54:08.21 Hand.open @14:54:08.91 Forearm.reconfigure :loose @14:54:08.95 Forearm.seek :retract @14:54:09.93 Completed [initialPurge; standAloneWCR([nil,100], $da)] begun 14:28:59 @14:54:09.98 Asleep after completing standAloneWCR_010224 assays @14:54:10.00 GoodNews.email "{1 => 1,\n 2 => 0}\nCTD data unavailable\nCan@14:48:12, 26% flow, 22.9C, 9% humidity, 15.0psia, 13.739V, 0.478A, 6.963Ah, 6.56W\nSampled 100.0ml",Subject: "Asleep after completing standAloneWCR_010224 assays" @14:54:14.38 upsync + ESPwhich standAloneWCR_010224.rb + fn=standAloneWCR_010224.rb + IFS=: + set /home/esp/esp2/mission . + fullPath=/home/esp/esp2/mission/standAloneWCR_010224.rb + [ -f /home/esp/esp2/mission/standAloneWCR_010224.rb ] + realpath /home/esp/esp2/mission/standAloneWCR_010224.rb + return 0 + scriptPath=/home/esp/esp2local/mission/standAloneWCR_010224.rb + exec ruby /home/esp/esp2/lib/esp.rb /home/esp/esp2local/mission/standAloneWCR_010224.rb @13:59:47.59UTC08-Jan-24
ESP2 v3.42 I2C Gateway v3.20 for core bus @13:59:50.22 Configuration for short puck jake 1/11/21 by brent@mbari.org /home/esp/esp2/type/shallow/jake/configure.rb @13:59:53.88 Polling Can every 10:00 @13:59:53.94 Loading /home/esp/esp2local/mission/standAloneWCR_010224.rb mission @13:59:53.99 Preparing to sleep until + 00:00:05 @13:59:54.02 GoodNews.email "Starting standAloneWCR_010224 w/pucks in tube 2\n{2 => 1}\nCTD data unavailable\nCan@13:59:53, 26% flow, 21.6C, 17% humidity, 15.0psia, 13.730V, 0.321A, 0.060Ah, 4.40W",Subject: "standAloneWCR_010224 begun" @13:59:54.08
Asleep til + 00:00:05 @13:59:54.10 Continuing at 13:59 @13:59:54.38 Gate.power :core,:OFF @13:59:54.39 Gate.power :raw,:OFF @13:59:54.40 Shutdown @13:59:57.63 GoodNews.email "Then, in tube 2: initialPurge; standAloneWCR([nil,100], $da)\n/home/esp/esp2local/mission/standAloneWCR_010224.rb:10\n{2 => 1}\nCTD data unavailable\nCan@13:59:54, 26% flow, 21.6C, 17% humidity, 15.1psia, 13.730V, 0.321A, 0.060Ah, 4.40W",Subject: "Asleep until 13:59" @13:59:59.00
Powering core bus @13:59:59.20 upsync @14:00:01.91 upsync @14:00:04.62
Gate.power :raw,:ON @14:00:05.72 CS.configure :default @14:00:05.77 PS.configure :default @14:00:05.81 SS.configure :default @14:00:05.86 CC.configure :default @14:00:05.91 PC.configure :default @14:00:05.97 Forearm.configure :loose @14:00:06.02 Elbow.configure :loose @14:00:06.09 SC.configure :loose @14:00:06.14 SE.configure :default @14:00:06.19 CH.configure :default @14:00:06.22 PH.configure :default @14:00:06.26 All dwarf controllers running firmware version 3.85 @14:00:06.37 Valve.reset @14:00:06.62 Intake.select :close @14:00:06.65 Exhaust.select :close @14:00:06.68 CTV.dial :air @14:00:06.80 PSV.dial :air @14:00:06.85 CSV.dial :waste1 @14:00:06.97 PS.home.seek 0ml @14:00:07.00
Forearm.home.seek :retract @14:00:07.33 SSV.dial :spit,avoiding: :exhaust @14:00:07.51 CS.reconfigure :fast @14:00:07.54 SS.home.seek 0ml @14:00:07.63 CS.home.seek 0ml @14:00:07.74 Hand.close @14:00:07.77
Elbow.reconfigure :loose @14:00:07.86 Elbow.jog 1500 @14:00:08.38 Elbow.reconfigure :loose @14:00:08.45 Elbow.home.seek :clear @14:00:11.70 SE.home.seek :down @14:00:12.31 SC.reconfigure :tight @14:00:12.36 SC.home.seek :initial @14:00:15.34 PS.stop @14:00:37.56
SC.configure :limp @14:00:37.61 Forearm.seek :retract @14:00:37.68 Elbow.seek :hammer @14:00:38.72 Forearm.reconfigure :tight @14:00:38.77 Forearm.seek retracted @14:00:39.05 PC.home.seek :open @14:00:39.07
CC.home.seek :open @14:00:42.59 Phase [initialPurge; standAloneWCR([nil,100], $da)] @14:00:42.61 Notice.email "At 14:00:42 began in tube 2: initialPurge; standAloneWCR([nil,100], $da)\n/home/esp/esp2local/mission/standAloneWCR_010224.rb:10\n{2 => 1}\nCTD data unavailable\nCan@14:00:42, 26% flow, 21.7C, 17% humidity, 15.0psia, 13.744V, 0.339A, 0.065Ah, 4.66W",Subject: "Started standAloneWCR_010224 phase initialPurge; standAloneWCR([nil,100], $da)" @14:00:42.64
Starting initialPurge @14:00:42.65 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:00:42.72 Hand.open @14:00:43.45 Elbow.reconfigure :tight @14:00:43.49 Elbow.seek FlushPuck::Garage @14:00:45.34 Elbow.reconfigure :loose @14:00:45.46 Forearm.reconfigure :loose @14:00:45.59 Forearm.seek :garage @14:00:46.58 Hand.close @14:00:46.92 upsync @14:00:47.32
Forearm.seek :retract @14:00:48.30 Elbow.reconfigure :tight @14:00:48.35 Elbow.seek CC @14:00:51.29 Elbow.reconfigure :loose @14:00:51.39 Forearm.reconfigure :tight @14:00:51.45 Forearm.seek CC @14:00:52.73 CC.seek :closed @14:00:57.28 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.43 @14:00:57.34 Hand.open @14:00:58.00 Forearm.reconfigure :loose @14:00:58.05 Forearm.seek :retract @14:00:59.07 Flushing the Collection Valve::Manifold @14:00:59.20 CSV.dial CSR @14:01:00.45 Pulling 4.999ml of CSR.flush @14:01:00.46 CSR.select :flush @14:01:00.58 CS.reconfigure :default @14:01:00.63 CS.seek 5ml @14:01:14.23 delay 5 seconds @14:01:19.25 CSR.select :air @14:01:19.83 CS.reconfigure :fast @14:01:19.88 CS.seek 7.5ml @14:01:26.61 CSV.dial :waste @14:01:27.90 CS.reconfigure :default @14:01:27.94 CS.seek 0ml @14:01:48.02 Purging external Intake @14:01:48.14 CSV.dial :flush @14:01:48.58 CS.reconfigure :fast @14:01:48.63 CS.seek 10ml @14:02:13.45 delay 5 seconds @14:02:18.47 CSV.dial CBV @14:02:18.52
CBV.dial CSV @14:02:19.23 CTV.dial :intake @14:02:20.01 Intake.select :open @14:02:20.12 CS.seek 0ml @14:02:43.70 Intake.select :close @14:02:43.81 CTV.dial :air @14:02:44.58 Flushing upper manifold @14:02:44.70 CSV.dial :flush @14:02:45.55 CS.seek 5ml @14:02:58.58 delay 5 seconds @14:03:03.60 CSV.dial :air @14:03:04.12 CS.seek 6.999ml @14:03:09.64 CSV.dial CBV @14:03:09.67
CBV.dial CSV @14:03:10.70 CTV.dial :air @14:03:10.78 CS.seek 0ml @14:03:27.54 delay 7 seconds @14:03:34.57 CS.seek 3ml @14:03:42.67 CSV.dial :waste @14:03:43.13 CS.seek 0ml @14:03:50.99 CSV.dial :flush @14:03:51.44 CS.seek 3ml @14:03:59.48 delay 5 seconds @14:04:04.50 CSV.dial :air @14:04:04.99 CS.seek 4.999ml @14:04:10.57 CSV.dial :air @14:04:10.66 CS.seek 0ml @14:04:22.95 delay 7 seconds @14:04:29.98 CS.seek 3ml @14:04:38.07 CSV.dial :waste @14:04:38.82 CS.seek 0ml @14:04:46.51 Garaging FlushPuck in Collection Clamp @14:04:46.58 Elbow.reconfigure :tight @14:04:46.63 Elbow.seek CC @14:04:46.96 Elbow.reconfigure :loose @14:04:47.06 Forearm.seek CC @14:04:48.09 Hand.close @14:04:48.69 CC.seek :open @14:04:53.71 Forearm.seek :retract @14:04:54.74 Elbow.reconfigure :tight @14:04:54.78 Elbow.seek :garage @14:04:57.65 Elbow.reconfigure :loose @14:04:57.75 Forearm.reconfigure :tight @14:04:57.79 Forearm.seek :garage @14:04:58.84 Hand.open @14:04:59.50 Forearm.reconfigure :loose @14:04:59.54 Forearm.seek :retract @14:05:00.48 Starting stand-alone da WCR @14:05:00.49 da wcr sampling at most 100ml @14:05:00.53 Whole Cell Archive sampling 100ml @14:05:00.54 DAfans.email "Started stand-alone da WCR in tube 2 at 14:05:00UTC08-Jan-24\n{2 => 1}\nCTD data unavailable\nCan@14:00:42, 26% flow, 21.7C, 17% humidity, 15.0psia, 13.744V, 0.339A, 0.065Ah, 4.66W",Subject: "da wcr sampling at most 100ml" @14:05:00.57 Loading wcr puck from tube 2 into Collection Clamp @14:05:00.82 SC.reconfigure :tight @14:05:00.89 SC.seek 2 @14:05:04.77 upsync @14:05:07.58 SC.configure :limp @14:05:07.82 Elbow.reconfigure :tight @14:05:07.87 Elbow.seek 2 @14:05:09.82 Forearm.reconfigure :tight @14:05:09.87 Forearm.seek 2 + 450 counts @14:05:10.89 SE.reconfigure :pick @14:05:10.96 SE.seek :beamBreak @14:05:14.05 SE.configure :default @14:05:14.21 Elbow.seek 2 @14:05:14.41 Forearm.seek 2 + 300 counts @14:05:14.61 Forearm.reconfigure :loose @14:05:14.66 Hand.close @14:05:15.33 SE.seek :down @14:05:15.91 Forearm.seek :retract @14:05:16.92 Elbow.seek CC @14:05:18.06 SE.stop @14:05:18.71 Elbow.reconfigure :loose @14:05:18.81 Forearm.reconfigure :tight @14:05:18.86 Forearm.seek CC @14:05:20.09 CC.seek :closed @14:05:24.61 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.34 @14:05:24.63 Hand.open @14:05:25.25 Forearm.reconfigure :loose @14:05:25.31 Forearm.seek :retract @14:05:26.31 collectSample 100ml w/bubble point of 35psi @14:05:26.33 Priming 7ml @14:05:26.34 Intake.select :close @14:05:26.35 Exhaust.select :close @14:05:26.36 SS.hold @14:05:26.57 Calibrating pressure and temperature sensors @14:05:26.60 CTV.dial :air @14:05:26.62 SSV.dial :spit,avoiding: :exhaust @14:05:26.70 SS.reconfigure :fast @14:05:26.75 SS.seek 5ml @14:05:29.65 SSV.dial CBV @14:05:29.69 CBV.dial SSV @14:05:31.03 delay 3.8 seconds @14:05:34.95 Sensor limits: Intake max 109.3psia; Outlet max 103.2psia @14:05:34.96 SSV.dial :spit @14:05:36.27 SS.seek 0ml @14:05:39.15 SSV.dial CBV @14:05:40.46 CTV.dial :intake @14:05:41.25 SSV.dial CBV @14:05:41.28 CBV.dial SSV @14:05:41.43 Allowing 40s for 7.0ml of remaining 7.0ml @14:05:41.44 Intake.select :open @14:05:43.60 Intake @15.4psia, vacuum from 9.9 to 12.9psi @14:05:43.62 SS.reconfigure :inhaling 2.20ml/s @14:05:43.67 SS.seek 7ml @14:05:50.55 Stroke completed -- waiting for pressure to equalize ... @14:05:50.56 SS.reconfigure :exhale @14:05:50.61 SS.hold @14:05:53.92 Intake.select :close @14:05:54.08 SSV.dial :exhaust @14:05:54.84 Exhaust.select :open @14:05:54.95 SS.seek 0ml @14:05:59.12 Exhaust.select :close @14:05:59.23 Sampling 100.0ml @14:05:59.24 Intake.select :close @14:05:59.25 Exhaust.select :close @14:05:59.27 SS.reconfigure :default @14:05:59.31 SS.hold @14:05:59.52 CTV.dial :intake @14:05:59.75 SSV.dial CBV @14:05:59.77 CBV.dial SSV @14:06:00.57 Allowing 130s for 25.0ml of remaining 100.0ml @14:06:00.58 Intake.select :open @14:06:02.74 Intake @15.4psia, vacuum from 9.9 to 12.9psi @14:06:02.76 SS.reconfigure :inhaling 2.20ml/s @14:06:02.81 SS.seek 25ml @14:06:16.36 Vacuum too high -- waiting up to 40 seconds @14:06:16.37 SS.reconfigure :exhale @14:06:16.43 SS.hold @14:06:17.12 Intake @12.4psia, vacuum from 6.9 to 9.9psi @14:06:17.13 SS.reconfigure :inhaling 1.76ml/s @14:06:17.18 SS.seek 25ml @14:06:17.25 Vacuum too high -- waiting up to 40 seconds @14:06:17.27 SS.reconfigure :exhale @14:06:17.32 SS.hold @14:06:22.97 Intake @13.6psia, vacuum from 8.1 to 11.1psi @14:06:22.99 SS.reconfigure :inhaling 1.41ml/s @14:06:23.04 SS.seek 25ml @14:06:25.97 Stroke completed -- waiting for pressure to equalize ... @14:06:25.99 SS.reconfigure :exhale @14:06:26.03 SS.hold @14:06:27.28 Intake.select :close @14:06:27.44 SSV.dial :exhaust @14:06:28.18 Exhaust.select :open @14:06:28.29 SS.seek 0ml @14:06:37.15 Exhaust.select :close @14:06:37.26 SSV.dial CBV @14:06:37.28 CBV.dial SSV @14:06:38.07 Allowing 130s for 25.0ml of remaining 75.0ml @14:06:38.08 Intake.select :open @14:06:40.25 Intake @15.6psia, vacuum from 10.0 to 13.0psi @14:06:40.26 SS.reconfigure :inhaling 1.41ml/s @14:06:40.31 SS.seek 25ml @14:07:01.66 Stroke completed -- waiting for pressure to equalize ... @14:07:01.67 SS.reconfigure :exhale @14:07:01.72 SS.hold @14:07:08.12 Intake.select :close @14:07:08.27 SSV.dial :exhaust @14:07:09.02 Exhaust.select :open @14:07:09.56 SS.seek 0ml @14:07:18.41 Exhaust.select :close @14:07:18.52 SSV.dial CBV @14:07:18.54 CBV.dial SSV @14:07:19.32 Allowing 130s for 25.0ml of remaining 50.0ml @14:07:19.33 Intake.select :open @14:07:21.49 Intake @15.6psia, vacuum from 10.0 to 13.0psi @14:07:21.51 SS.reconfigure :inhaling 1.41ml/s @14:07:21.56 SS.seek 25ml @14:07:42.83 Stroke completed -- waiting for pressure to equalize ... @14:07:42.84 SS.reconfigure :exhale @14:07:42.89 SS.hold @14:07:50.36 Intake.select :close @14:07:50.52 SSV.dial :exhaust @14:07:51.25 Exhaust.select :open @14:07:51.37 SS.seek 0ml @14:08:00.25 Exhaust.select :close @14:08:00.36 SSV.dial CBV @14:08:00.39 CBV.dial SSV @14:08:01.16 Allowing 130s for 25.0ml of remaining 25.0ml @14:08:01.17 Intake.select :open @14:08:03.34 Intake @15.5psia, vacuum from 10.0 to 13.0psi @14:08:03.36 SS.reconfigure :inhaling 1.41ml/s @14:08:03.41 SS.seek 25ml @14:08:24.71 Stroke completed -- waiting for pressure to equalize ... @14:08:24.72 SS.reconfigure :exhale @14:08:24.76 SS.hold @14:08:33.73 Intake.select :close @14:08:33.88 SSV.dial :exhaust @14:08:34.63 Exhaust.select :open @14:08:34.75 SS.seek 0ml @14:08:43.64 Exhaust.select :close @14:08:43.76 Sampled 100.0ml @14:08:43.77 Drying puck before fixing for 5:00 @14:08:43.78 CBV.dial :waste2b @14:08:44.52 delay 1:00 @14:09:44.55 CSV.dial :air @14:09:45.30 CS.seek 10ml @14:10:09.85 Pumping wcr puck from tube 2 up to 35psi... @14:10:09.87 CBV.dial SSV @14:10:11.12 CBV.dial between CSV and waste2b @14:10:12.06 CSV.dial between air and CTV @14:10:12.37 CS.seek 7.441ml @14:10:19.04 CTV.dial CSV,avoiding: :air @14:10:19.07 CSV.dial CTV @14:10:19.90 CS.reconfigure :pressurize @14:10:19.95 CS.seek 0ml @14:10:39.50 CSV.dial :air @14:10:39.96 CS.reconfigure :fast @14:10:40.02 CS.seek 10ml @14:11:04.34 CSV.dial between air and CTV @14:11:04.67 CS.seek 3.319ml @14:11:20.88 CTV.dial CSV,avoiding: :air @14:11:20.91 CSV.dial CTV @14:11:21.67 CS.reconfigure :pressurize @14:11:21.78 CS.seek 0ml @14:11:24.99 CBV.dial :waste2b @14:11:25.32 CS.reconfigure :default @14:11:25.37 CS.seek 0ml @14:11:32.10 delay 1:00 @14:12:32.13 CTV.dial :air,avoiding: :intake @14:12:33.46 Fix #1 of 2 (with RNAlater) for 2:30 @14:12:33.48 CSV.dial CSR @14:12:33.91 Pulling 2ml of CSR.RNAlater @14:12:33.92 CSR.select :RNAlater @14:12:34.04 CS.seek 2ml @14:12:40.03 delay 5 seconds @14:12:45.06 CSR.select :air @14:12:45.17 CS.reconfigure :fast @14:12:45.22 CS.seek 10ml @14:13:04.88 Pumping wcr puck from tube 2 up to 35psi... @14:13:04.90 CBV.dial SSV @14:13:06.14 CBV.dial between CSV and waste2b @14:13:07.10 CSV.dial between air and CTV @14:13:07.70 CS.seek 7.303ml @14:13:14.70 CTV.dial CSV,avoiding: :air @14:13:14.72 CSV.dial CTV @14:13:16.09 CS.reconfigure :pressurize @14:13:16.14 CS.seek 0ml @14:13:34.65 delay 2:30 @14:16:04.67 CBV.dial :waste2b @14:16:05.00 CS.reconfigure :default @14:16:05.06 CS.seek 0ml @14:16:06.44 delay 1:00 @14:17:06.47 CTV.dial :air,avoiding: :intake @14:17:07.77 Fix #2 of 2 (with RNAlater) for 2:30 @14:17:07.78 CSV.dial CSR @14:17:08.33 Pulling 2ml of CSR.RNAlater @14:17:08.34 CSR.select :RNAlater @14:17:08.45 CS.seek 2ml @14:17:14.62 delay 5 seconds @14:17:19.64 CSR.select :air @14:17:19.76 CS.reconfigure :fast @14:17:19.81 CS.seek 10ml @14:17:39.47 Pumping wcr puck from tube 2 up to 35psi... @14:17:39.49 CBV.dial SSV @14:17:40.72 CBV.dial between CSV and waste2b @14:17:41.68 CSV.dial between air and CTV @14:17:42.27 CS.seek 7.252ml @14:17:49.63 CTV.dial CSV,avoiding: :air @14:17:49.65 CSV.dial CTV @14:17:51.00 CS.reconfigure :pressurize @14:17:51.06 CS.seek 0ml @14:18:07.36 delay 2:30 @14:20:37.80 CBV.dial :waste2b @14:20:38.16 CS.reconfigure :default @14:20:38.21 CS.seek 0ml @14:20:41.65 delay 1:00 @14:21:41.68 CTV.dial :air,avoiding: :intake @14:21:43.04 Storing wcr puck in Collection Clamp to tube 1 @14:21:43.21 SC.reconfigure :tight @14:21:43.26 SC.seek 1 @14:21:46.82 SC.configure :limp @14:21:47.04 Elbow.reconfigure :tight @14:21:47.09 Elbow.seek 1 @14:21:48.79 Forearm.reconfigure :tight @14:21:48.85 Forearm.seek 1 + 450 counts @14:21:49.87 SE.reconfigure :pick @14:21:49.93 SE.seek :beamBreak @14:21:52.77 SE.configure :default @14:21:52.82 SE.seek :place @14:21:53.37 Forearm.reconfigure :loose @14:21:53.41 Forearm.seek :retract @14:21:54.42 Elbow.seek CC @14:21:56.10 Elbow.reconfigure :loose @14:21:56.20 Forearm.seek CC @14:21:57.20 Hand.close @14:21:57.81 CC.seek :open @14:22:02.54 Forearm.seek :retract @14:22:03.53 Elbow.reconfigure :tight @14:22:03.58 Elbow.seek 1 @14:22:05.34 Elbow.reconfigure :limp @14:22:05.40 Forearm.seek 1 @14:22:06.39 Elbow.hold @14:22:06.89 Elbow.reconfigure :tight @14:22:06.94 Hand.open @14:22:07.67 Forearm.force -20 @14:22:07.71 Forearm.seek 1 + 310 counts @14:22:08.00 Forearm.reconfigure :tight @14:22:08.05 Forearm.seek 1 + 450 counts @14:22:08.30 SE.seek :down @14:22:09.45 Forearm.reconfigure :loose @14:22:09.50 Forearm.seek :retract @14:22:11.05 SE.stop @14:22:11.09
Starting finalPurge @14:22:11.11 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:22:11.30 Elbow.seek FlushPuck::Garage @14:22:13.10 Elbow.reconfigure :loose @14:22:13.20 Forearm.seek :garage @14:22:14.15 Hand.close @14:22:14.92 Forearm.seek :retract @14:22:15.91 Elbow.reconfigure :tight @14:22:15.95 Elbow.seek CC @14:22:18.88 Elbow.reconfigure :loose @14:22:18.98 Forearm.reconfigure :tight @14:22:19.03 Forearm.seek CC @14:22:20.35 CC.seek :closed @14:22:24.87 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.32 @14:22:24.94 Hand.open @14:22:26.06 Forearm.reconfigure :loose @14:22:26.11 Forearm.seek :retract @14:22:27.09 Flushing the Collection Valve::Manifold @14:22:27.22 CSV.dial CSR @14:22:27.76 Pulling 5ml of CSR.flush @14:22:27.77 CSR.select :flush @14:22:27.88 CS.seek 5ml @14:22:41.59 delay 5 seconds @14:22:46.61 CSR.select :air @14:22:46.77 CS.reconfigure :fast @14:22:46.82 CS.seek 7.5ml @14:22:53.62 CSV.dial :waste @14:22:54.89 CS.reconfigure :default @14:22:54.94 CS.seek 0ml @14:23:15.15 Flushing lower manifold's waste2b port @14:23:15.27 CSV.dial :flush @14:23:15.73 CS.reconfigure :fast @14:23:15.78 CS.seek 5ml @14:23:28.40 delay 5 seconds @14:23:33.42 CSV.dial :air @14:23:33.91 CS.seek 7.001ml @14:23:39.49 CSV.dial CTV @14:23:39.52
CTV.dial CSV @14:23:40.87 CBV.dial :waste2b @14:23:40.95 CS.seek 0ml @14:23:57.53 Disinfecting external Intake with kill @14:23:57.65 CSV.dial CSR @14:23:58.09 Pulling 8ml of CSR.kill CSR.select :kill @14:23:58.21 CS.seek 8ml @14:24:17.90 delay 5 seconds @14:24:22.92 CSR.select :air @14:24:23.04 CS.seek 10ml @14:24:28.59 CSV.dial CBV @14:24:28.63
CBV.dial CSV @14:24:29.65 CTV.dial :intake @14:24:30.41 Intake.select :open @14:24:30.52 CS.seek 2ml @14:24:49.24 Intake.select :close @14:24:49.34 CTV.dial :air @14:24:50.07 CS.reconfigure :default @14:24:50.12 CS.seek 0ml @14:24:55.99 CSV.dial CSR @14:24:57.12 Pulling 5ml of CSR.flush CSR.select :flush @14:24:57.24 CS.seek 5ml @14:25:11.05 delay 5 seconds @14:25:16.07 CSR.select :air @14:25:16.19 CS.seek 10ml @14:25:29.89 CSV.dial CBV @14:25:30.89 CS.seek 0ml @14:25:57.37 Garaging FlushPuck in Collection Clamp @14:25:57.44 Elbow.reconfigure :tight @14:25:57.48 Elbow.seek CC @14:25:58.17 Elbow.reconfigure :loose @14:25:58.27 Forearm.seek CC @14:25:59.28 Hand.close @14:25:59.92 CC.seek :open @14:26:04.57 Forearm.seek :retract @14:26:05.58 Elbow.reconfigure :tight @14:26:05.62 Elbow.seek :garage @14:26:08.55 Elbow.reconfigure :loose @14:26:08.65 Forearm.reconfigure :tight @14:26:08.70 Forearm.seek :garage @14:26:09.74 Hand.open @14:26:10.35 Forearm.reconfigure :loose @14:26:10.40 Forearm.seek :retract @14:26:11.39 Completed [initialPurge; standAloneWCR([nil,100], $da)] begun 14:00:42 @14:26:11.44 Asleep after completing standAloneWCR_010224 assays @14:26:11.45 GoodNews.email "{1 => 1,\n 2 => 0}\nCTD data unavailable\nCan@14:19:55, 26% flow, 22.7C, 16% humidity, 15.0psia, 13.773V, 0.478A, 0.221Ah, 6.58W\nSampled 100.0ml",Subject: "Asleep after completing standAloneWCR_010224 assays" @14:26:15.80 upsync + ESPwhich DA_WCR_010224.rb + fn=DA_WCR_010224.rb + IFS=: + set /home/esp/esp2/mission . + fullPath=/home/esp/esp2/mission/DA_WCR_010224.rb + [ -f /home/esp/esp2/mission/DA_WCR_010224.rb ] + fullPath=./DA_WCR_010224.rb + [ -f ./DA_WCR_010224.rb ] + return 1 + scriptPath= + [ DA_WCR_010224.rb = DA_WCR_010224 ] + echo No 'DA_WCR_010224.rb' script found on ESPpath: No 'DA_WCR_010224.rb' script found on ESPpath: + echo /home/esp/esp2/mission:. /home/esp/esp2/mission:. + exit 1 + ESPwhich standAloneWCR__10224.rb + fn=standAloneWCR__10224.rb + IFS=: + set /home/esp/esp2/mission . + fullPath=/home/esp/esp2/mission/standAloneWCR__10224.rb + [ -f /home/esp/esp2/mission/standAloneWCR__10224.rb ] + fullPath=./standAloneWCR__10224.rb + [ -f ./standAloneWCR__10224.rb ] + return 1 + scriptPath= + [ standAloneWCR__10224.rb = standAloneWCR__10224 ] + echo No 'standAloneWCR__10224.rb' script found on ESPpath: No 'standAloneWCR__10224.rb' script found on ESPpath: + echo /home/esp/esp2/mission:. /home/esp/esp2/mission:. + exit 1 + ESPwhich standAloneWCR_010224.rb + fn=standAloneWCR_010224.rb + IFS=: + set /home/esp/esp2/mission . + fullPath=/home/esp/esp2/mission/standAloneWCR_010224.rb + [ -f /home/esp/esp2/mission/standAloneWCR_010224.rb ] + realpath /home/esp/esp2/mission/standAloneWCR_010224.rb + return 0 + scriptPath=/home/esp/esp2local/mission/standAloneWCR_010224.rb + exec ruby /home/esp/esp2/lib/esp.rb /home/esp/esp2local/mission/standAloneWCR_010224.rb @15:10:47.61UTC08-Jan-24
ESP2 v3.42 I2C Gateway v3.20 for core bus @15:10:50.30 Configuration for short puck jake 1/11/21 by brent@mbari.org /home/esp/esp2/type/shallow/jake/configure.rb @15:10:54.05 Polling Can every 10:00 @15:10:54.11 Loading /home/esp/esp2local/mission/standAloneWCR_010224.rb mission @15:10:54.16 Preparing to sleep until + 00:00:05 @15:10:54.20 GoodNews.email "Starting standAloneWCR_010224 w/pucks in tube 2\n{2 => 1}\nCTD data unavailable\nCan@15:10:54, 26% flow, 22.1C, 17% humidity, 15.0psia, 13.822V, 0.284A, 0.496Ah, 3.93W",Subject: "standAloneWCR_010224 begun" @15:10:54.24
Asleep til + 00:00:05 @15:10:54.26 Continuing at 15:10 @15:10:54.50 Gate.power :core,:OFF @15:10:54.53 Gate.power :raw,:OFF @15:10:54.54 Shutdown @15:10:57.04 GoodNews.email "Then, in tube 2: initialPurge; standAloneWCR([nil,100], $da)\n/home/esp/esp2local/mission/standAloneWCR_010224.rb:10\n{2 => 1}\nCTD data unavailable\nCan@15:10:54, 26% flow, 22.1C, 17% humidity, 15.0psia, 13.822V, 0.284A, 0.496Ah, 3.93W",Subject: "Asleep until 15:10" @15:10:58.38 upsync @15:10:59.14
Powering core bus @15:11:01.08 upsync @15:11:04.57
Gate.power :raw,:ON @15:11:05.77 CS.configure :default @15:11:05.82 PS.configure :default @15:11:05.87 SS.configure :default @15:11:05.91 CC.configure :default @15:11:05.96 PC.configure :default @15:11:06.03 Forearm.configure :loose @15:11:06.08 Elbow.configure :loose @15:11:06.15 SC.configure :loose @15:11:06.19 SE.configure :default @15:11:06.24 CH.configure :default @15:11:06.28 PH.configure :default @15:11:06.32 All dwarf controllers running firmware version 3.85 @15:11:06.43 Valve.reset @15:11:06.73 Intake.select :close @15:11:06.76 PSV.dial :air @15:11:06.79 Exhaust.select :close @15:11:06.86 CTV.dial :air @15:11:06.97
Forearm.home.seek :retract @15:11:07.01 CSV.dial :waste1 @15:11:07.02 PS.home.seek 0ml @15:11:07.55 SSV.dial :spit,avoiding: :exhaust @15:11:07.80 Hand.close @15:11:07.84
Elbow.reconfigure :loose @15:11:07.92 Elbow.jog 1500 @15:11:08.40 CS.reconfigure :fast @15:11:08.43 SS.home.seek 0ml @15:11:08.53 CS.home.seek 0ml @15:11:08.61
Elbow.reconfigure :loose @15:11:08.66 Elbow.home.seek :clear @15:11:11.91 SE.home.seek :down @15:11:12.52 SC.reconfigure :tight @15:11:12.56 SC.home.seek :initial @15:11:15.29 PS.stop @15:11:37.75
SC.configure :limp @15:11:37.80 Forearm.seek :retract @15:11:37.86 Elbow.seek :hammer @15:11:38.89 Forearm.reconfigure :tight @15:11:38.94 Forearm.seek retracted @15:11:39.23 PC.home.seek :open @15:11:39.25
CC.home.seek :open @15:11:42.70 Phase [initialPurge; standAloneWCR([nil,100], $da)] @15:11:42.71 Notice.email "At 15:11:42 began in tube 2: initialPurge; standAloneWCR([nil,100], $da)\n/home/esp/esp2local/mission/standAloneWCR_010224.rb:10\n{2 => 1}\nCTD data unavailable\nCan@15:11:42, 26% flow, 22.1C, 17% humidity, 15.0psia, 13.754V, 0.309A, 0.501Ah, 4.24W",Subject: "Started standAloneWCR_010224 phase initialPurge; standAloneWCR([nil,100], $da)" @15:11:42.74
Starting initialPurge @15:11:42.76 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @15:11:42.83 Hand.open @15:11:43.60 Elbow.reconfigure :tight @15:11:43.65 Elbow.seek FlushPuck::Garage @15:11:45.40 Elbow.reconfigure :loose @15:11:45.52 Forearm.reconfigure :loose @15:11:45.57 Forearm.seek :garage @15:11:46.61 Hand.close @15:11:46.96 upsync @15:11:47.33
Forearm.seek :retract @15:11:48.32 Elbow.reconfigure :tight @15:11:48.37 Elbow.seek CC @15:11:51.29 Elbow.reconfigure :loose @15:11:51.40 Forearm.reconfigure :tight @15:11:51.45 Forearm.seek CC @15:11:52.75 CC.seek :closed @15:11:57.27 Puck::Warning in MAIN -- Retaining claim on Arm because Hand is likely holding a puck! @15:11:57.29 Slide::Error in MAIN -- Collection Clamp overCurrent at closed @15:11:57.31 Waiting to Resume from Checkpoint "Collection Clamp overCurrent at closed" @15:11:57.32 BadNews.email "Collection Clamp overCurrent at closed",Subject: "Slide::Error in MAIN" @15:12:01.27 upsync @15:13:52.26 Session Begun @15:13:59.57 -> MianThread.resume @15:13:59.59 NameError in dave -- uninitialized constant MianThread @15:14:07.47 -> MainThread.resume @15:14:07.48
Resuming Thread after Collection Clamp overCurrent at closed at 15:11:57 # @15:14:08.18 CC.seek :open @15:14:12.76 CC.seek :closed @15:14:17.29 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.35 @15:14:17.37 Hand.open @15:14:18.01 Forearm.reconfigure :loose @15:14:18.08 Forearm.seek :retract @15:14:19.11 Flushing the Collection Valve::Manifold @15:14:19.26 CSV.dial CSR @15:14:20.66 Pulling 5ml of CSR.flush CSR.select :flush @15:14:20.85 CS.reconfigure :default @15:14:20.91 CS.seek 5ml @15:14:25.41 Session Ended @15:14:34.81
delay 5 seconds @15:14:39.83 CSR.select :air @15:14:39.99 CS.reconfigure :fast @15:14:40.04 CS.seek 7.5ml @15:14:46.81 CSV.dial :waste @15:14:48.08 CS.reconfigure :default @15:14:48.12 CS.seek 0ml @15:15:08.12 Purging external Intake @15:15:08.25 CSV.dial :flush @15:15:08.70 CS.reconfigure :fast @15:15:08.75 CS.seek 10ml @15:15:33.21 delay 5 seconds @15:15:38.24 CSV.dial CBV @15:15:38.28
CBV.dial CSV @15:15:38.99 CTV.dial :intake @15:15:39.76 Intake.select :open @15:15:39.87 CS.seek 0ml @15:16:03.24 Intake.select :close @15:16:03.35 CTV.dial :air @15:16:04.12 Flushing upper manifold @15:16:04.24 CSV.dial :flush @15:16:04.98 CS.seek 5ml @15:16:17.71 delay 5 seconds @15:16:22.73 CSV.dial :air @15:16:23.23 CS.seek 7ml @15:16:28.81 CSV.dial CBV @15:16:28.83
CBV.dial CSV @15:16:29.87 CTV.dial :air @15:16:30.11 CS.seek 0ml @15:16:46.68 delay 7 seconds @15:16:54.12 CS.seek 3ml @15:17:02.30 CSV.dial :waste @15:17:02.76 CS.seek 0ml @15:17:10.79 CSV.dial :flush @15:17:11.23 CS.seek 3ml @15:17:19.34 delay 5 seconds @15:17:24.35 CSV.dial :air @15:17:24.85 CS.seek 5.001ml @15:17:30.49 CSV.dial :air @15:17:30.58 CS.seek 0ml @15:17:42.77 delay 7 seconds @15:17:49.80 CS.seek 3ml @15:17:58.05 CSV.dial :waste @15:17:58.80 CS.seek 0ml @15:18:06.46 Garaging FlushPuck in Collection Clamp @15:18:06.53 Elbow.reconfigure :tight @15:18:06.58 Elbow.seek CC @15:18:06.82 Elbow.reconfigure :loose @15:18:06.91 Forearm.seek CC @15:18:07.92 Hand.close @15:18:08.58 CC.seek :open @15:18:13.23 Forearm.seek :retract @15:18:14.20 Elbow.reconfigure :tight @15:18:14.25 Elbow.seek :garage @15:18:17.21 Elbow.reconfigure :loose @15:18:17.31 Forearm.reconfigure :tight @15:18:17.36 Forearm.seek :garage @15:18:18.30 Hand.open @15:18:19.01 Forearm.reconfigure :loose @15:18:19.05 Forearm.seek :retract @15:18:20.01 Starting stand-alone da WCR @15:18:20.02 da wcr sampling at most 100ml @15:18:20.05 Whole Cell Archive sampling 100ml @15:18:20.07 DAfans.email "Started stand-alone da WCR in tube 2 at 15:18:20UTC08-Jan-24\n{2 => 1}\nCTD data unavailable\nCan@15:11:42, 26% flow, 22.1C, 17% humidity, 15.0psia, 13.754V, 0.309A, 0.501Ah, 4.24W",Subject: "da wcr sampling at most 100ml" @15:18:20.11 Loading wcr puck from tube 2 into Collection Clamp @15:18:20.37 SC.reconfigure :tight @15:18:20.42 SC.seek 2 @15:18:24.31 upsync @15:18:27.10 SC.configure :limp @15:18:27.79 Elbow.reconfigure :tight @15:18:27.84 Elbow.seek 2 @15:18:29.76 Forearm.reconfigure :tight @15:18:29.81 Forearm.seek 2 + 450 counts @15:18:30.85 SE.reconfigure :pick @15:18:30.91 SE.seek :beamBreak @15:18:34.00 SE.configure :default @15:18:34.16 Elbow.seek 2 @15:18:34.35 Forearm.seek 2 + 300 counts @15:18:34.56 Forearm.reconfigure :loose @15:18:34.61 Hand.close @15:18:35.21 SE.seek :down @15:18:35.42 Forearm.seek :retract @15:18:36.37 Elbow.seek CC @15:18:38.01 Elbow.reconfigure :loose @15:18:38.04 SE.stop @15:18:38.18 Forearm.reconfigure :tight @15:18:38.23 Forearm.seek CC @15:18:39.48 CC.seek :closed @15:18:44.00 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.20 @15:18:44.01 Hand.open @15:18:44.64 Forearm.reconfigure :loose @15:18:44.69 Forearm.seek :retract @15:18:45.67 collectSample 100ml w/bubble point of 35psi @15:18:45.69 Priming 7ml @15:18:45.70 Intake.select :close @15:18:45.71 Exhaust.select :close @15:18:45.72 SS.hold @15:18:46.37 Calibrating pressure and temperature sensors @15:18:46.40 CTV.dial :air @15:18:46.48 SSV.dial :spit,avoiding: :exhaust @15:18:46.56 SS.reconfigure :fast @15:18:46.61 SS.seek 5ml @15:18:49.54 SSV.dial CBV @15:18:49.57 CBV.dial SSV @15:18:50.90 delay 3.8 seconds @15:18:54.83 Sensor limits: Intake max 108.9psia; Outlet max 103.4psia @15:18:54.84 SSV.dial :spit @15:18:56.15 SS.seek 0ml @15:18:59.05 SSV.dial CBV @15:19:00.36 CTV.dial :intake @15:19:01.09 SSV.dial CBV @15:19:01.11 CBV.dial SSV @15:19:01.33 Allowing 40s for 7.0ml of remaining 7.0ml @15:19:01.34 Intake.select :open @15:19:03.52 Intake @15.4psia, vacuum from 9.9 to 12.9psi @15:19:03.54 SS.reconfigure :inhaling 2.20ml/s @15:19:03.59 SS.seek 7ml @15:19:10.72 Stroke completed -- waiting for pressure to equalize ... @15:19:10.73 SS.reconfigure :exhale @15:19:10.78 SS.hold @15:19:14.94 Intake.select :close @15:19:15.09 SSV.dial :exhaust @15:19:15.87 Exhaust.select :open @15:19:15.99 SS.seek 0ml @15:19:20.17 Exhaust.select :close @15:19:20.29 Sampling 100.0ml @15:19:20.30 Intake.select :close @15:19:20.31 Exhaust.select :close @15:19:20.32 SS.reconfigure :default @15:19:20.37 SS.hold @15:19:20.57 CTV.dial :intake @15:19:20.65 SSV.dial CBV @15:19:20.69 CBV.dial SSV @15:19:21.46 Allowing 130s for 25.0ml of remaining 100.0ml @15:19:21.47 Intake.select :open @15:19:23.63 Intake @15.6psia, vacuum from 10.0 to 13.0psi @15:19:23.65 SS.reconfigure :inhaling 2.20ml/s @15:19:23.70 SS.seek 25ml @15:19:38.92 Stroke completed -- waiting for pressure to equalize ... @15:19:38.93 SS.reconfigure :exhale @15:19:38.98 SS.hold @15:19:44.63 Intake.select :close @15:19:44.79 SSV.dial :exhaust @15:19:45.55 Exhaust.select :open @15:19:45.67 SS.seek 0ml @15:19:54.55 Exhaust.select :close @15:19:54.66 SSV.dial CBV @15:19:54.69 CBV.dial SSV @15:19:55.46 Allowing 130s for 25.0ml of remaining 75.0ml @15:19:55.47 Intake.select :open @15:19:57.64 Intake @15.7psia, vacuum from 10.0 to 13.0psi @15:19:57.66 SS.reconfigure :inhaling 2.20ml/s @15:19:57.71 SS.seek 25ml @15:20:12.84 Stroke completed -- waiting for pressure to equalize ... @15:20:12.86 SS.reconfigure :exhale @15:20:12.90 SS.hold @15:20:19.45 Intake.select :close @15:20:19.60 SSV.dial :exhaust @15:20:20.36 Exhaust.select :open @15:20:20.47 SS.seek 0ml @15:20:29.44 Exhaust.select :close @15:20:29.55 SSV.dial CBV @15:20:29.58 CBV.dial SSV @15:20:30.35 Allowing 130s for 25.0ml of remaining 50.0ml @15:20:30.36 Intake.select :open @15:20:32.52 Intake @15.7psia, vacuum from 10.0 to 13.0psi @15:20:32.54 SS.reconfigure :inhaling 2.20ml/s @15:20:32.59 SS.seek 25ml @15:20:46.68 Vacuum too high -- waiting up to 40 seconds @15:20:46.70 SS.reconfigure :exhale @15:20:46.75 SS.hold @15:20:47.45 Intake @12.5psia, vacuum from 7.0 to 10.0psi @15:20:47.47 SS.reconfigure :inhaling 1.76ml/s @15:20:47.52 SS.seek 25ml @15:20:50.15 Stroke completed -- waiting for pressure to equalize ... @15:20:50.17 SS.reconfigure :exhale @15:20:50.22 SS.hold @15:20:56.77 Intake.select :close @15:20:56.93 SSV.dial :exhaust @15:20:58.12 Exhaust.select :open @15:20:58.24 SS.seek 0ml @15:21:07.37 Exhaust.select :close @15:21:07.49 SSV.dial CBV @15:21:07.51 CBV.dial SSV @15:21:08.28 Allowing 130s for 25.0ml of remaining 25.0ml @15:21:08.29 Intake.select :open @15:21:10.46 Intake @15.7psia, vacuum from 10.0 to 13.0psi @15:21:10.48 SS.reconfigure :inhaling 1.76ml/s @15:21:10.53 SS.seek 25ml @15:21:27.65 Stroke completed -- waiting for pressure to equalize ... @15:21:27.67 SS.reconfigure :exhale @15:21:27.71 SS.hold @15:21:35.17 Intake.select :close @15:21:35.32 SSV.dial :exhaust @15:21:36.08 Exhaust.select :open @15:21:36.19 SS.seek 0ml @15:21:44.98 Exhaust.select :close @15:21:45.10 Sampled 100.0ml @15:21:45.12 Drying puck before fixing for 5:00 @15:21:45.13 CBV.dial :waste2b @15:21:45.88 delay 1:00 @15:22:45.91 CSV.dial :air @15:22:46.65 CS.seek 10ml @15:23:11.18 Pumping wcr puck from tube 2 up to 35psi... @15:23:11.19 CBV.dial SSV @15:23:12.45 CBV.dial between CSV and waste2b @15:23:13.39 CSV.dial between air and CTV @15:23:13.70 CS.seek 7.424ml @15:23:20.37 CTV.dial CSV,avoiding: :air @15:23:20.85 CSV.dial CTV @15:23:21.63 CS.reconfigure :pressurize @15:23:21.69 CS.seek 0ml @15:23:37.29 CBV.dial :waste2b @15:23:37.62 CS.reconfigure :default @15:23:37.67 CS.seek 0ml @15:23:42.31 delay 1:00 @15:24:42.34 CTV.dial :air,avoiding: :intake @15:24:43.63 Fix #1 of 2 (with RNAlater) for 2:30 @15:24:43.65 CSV.dial CSR @15:24:44.08 Pulling 2ml of CSR.RNAlater @15:24:44.09 CSR.select :RNAlater @15:24:44.20 CS.seek 2ml @15:24:50.17 delay 5 seconds @15:24:55.19 CSR.select :air @15:24:55.31 CS.reconfigure :fast @15:24:55.36 CS.seek 10ml @15:25:15.05 Pumping wcr puck from tube 2 up to 35psi... @15:25:15.07 CBV.dial SSV @15:25:16.30 CBV.dial between CSV and waste2b @15:25:17.26 CSV.dial between air and CTV @15:25:17.87 CS.seek 7.372ml @15:25:24.71 CTV.dial CSV,avoiding: :air @15:25:24.73 CSV.dial CTV @15:25:26.29 CS.reconfigure :pressurize @15:25:26.34 CS.seek 0ml @15:25:40.04 delay 2:30 @15:28:10.06 CBV.dial :waste2b @15:28:10.39 CS.reconfigure :default @15:28:10.44 CS.seek 0ml @15:28:16.83 delay 1:00 @15:29:16.85 CTV.dial :air,avoiding: :intake @15:29:18.19 Fix #2 of 2 (with RNAlater) for 2:30 @15:29:18.20 CSV.dial CSR @15:29:18.65 Pulling 2ml of CSR.RNAlater @15:29:18.66 CSR.select :RNAlater @15:29:18.78 CS.seek 2ml @15:29:24.70 delay 5 seconds @15:29:29.72 CSR.select :air @15:29:29.84 CS.reconfigure :fast @15:29:29.88 CS.seek 10ml @15:29:49.54 Pumping wcr puck from tube 2 up to 35psi... @15:29:49.56 CBV.dial SSV @15:29:50.81 CBV.dial between CSV and waste2b @15:29:51.76 CSV.dial between air and CTV @15:29:52.36 CS.seek 7.355ml @15:29:59.26 CTV.dial CSV,avoiding: :air @15:29:59.29 CSV.dial CTV @15:30:00.67 CS.reconfigure :pressurize @15:30:01.17 CS.seek 0ml @15:30:20.48 CSV.dial :air @15:30:20.92 CS.reconfigure :fast @15:30:20.98 CS.seek 10ml @15:30:45.54 CSV.dial between air and CTV @15:30:45.86 CS.seek 4.102ml @15:31:00.17 CTV.dial CSV,avoiding: :air @15:31:00.20 CSV.dial CTV @15:31:00.56 CS.reconfigure :pressurize @15:31:00.61 CS.seek 0ml @15:31:09.87 delay 2:30 @15:33:39.90 CBV.dial :waste2b @15:33:40.23 CS.reconfigure :default @15:33:40.28 CS.seek 0ml @15:33:42.94 delay 1:00 @15:34:42.97 CTV.dial :air,avoiding: :intake @15:34:44.32 Storing wcr puck in Collection Clamp to tube 1 @15:34:44.49 SC.reconfigure :tight @15:34:44.54 SC.seek 1 @15:34:48.11 SC.configure :limp @15:34:48.33 Elbow.reconfigure :tight @15:34:48.38 Elbow.seek 1 @15:34:50.14 Forearm.reconfigure :tight @15:34:50.20 Forearm.seek 1 + 450 counts @15:34:51.23 SE.reconfigure :pick @15:34:51.29 SE.seek :beamBreak @15:34:54.12 SE.configure :default @15:34:54.17 SE.seek :place @15:34:54.72 Forearm.reconfigure :loose @15:34:54.77 Forearm.seek :retract @15:34:55.73 Elbow.seek CC @15:34:57.38 Elbow.reconfigure :loose @15:34:57.48 Forearm.seek CC @15:34:58.48 Hand.close @15:34:59.48 CC.seek :open @15:35:04.31 Forearm.seek :retract @15:35:05.31 Elbow.reconfigure :tight @15:35:05.36 Elbow.seek 1 @15:35:07.14 Elbow.reconfigure :limp @15:35:07.20 Forearm.seek 1 @15:35:08.20 Elbow.hold @15:35:08.27 Elbow.reconfigure :tight @15:35:08.32 Hand.open @15:35:09.05 Forearm.force -20 @15:35:09.10 Forearm.seek 1 + 303 counts @15:35:09.38 Forearm.reconfigure :tight @15:35:09.43 Forearm.seek 1 + 450 counts @15:35:09.69 SE.seek :down @15:35:10.82 Forearm.reconfigure :loose @15:35:10.87 Forearm.seek :retract @15:35:12.36 SE.stop @15:35:12.41
Starting finalPurge @15:35:12.42 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @15:35:12.61 Elbow.seek FlushPuck::Garage @15:35:14.41 Elbow.reconfigure :loose @15:35:14.51 Forearm.seek :garage @15:35:15.50 Hand.close @15:35:16.18 Forearm.seek :retract @15:35:17.14 Elbow.reconfigure :tight @15:35:17.19 Elbow.seek CC @15:35:20.10 Elbow.reconfigure :loose @15:35:20.20 Forearm.reconfigure :tight @15:35:20.24 Forearm.seek CC @15:35:21.94 CC.seek :closed @15:35:26.47 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.39 @15:35:26.53 Hand.open @15:35:27.24 Forearm.reconfigure :loose @15:35:27.29 Forearm.seek :retract @15:35:28.29 Flushing the Collection Valve::Manifold @15:35:28.42 CSV.dial CSR @15:35:28.85 Pulling 4.999ml of CSR.flush @15:35:28.86 CSR.select :flush @15:35:28.97 CS.seek 5ml @15:35:42.54 delay 5 seconds @15:35:47.55 CSR.select :air @15:35:47.72 CS.reconfigure :fast @15:35:47.77 CS.seek 7.499ml @15:35:54.57 CSV.dial :waste @15:35:55.85 CS.reconfigure :default @15:35:55.90 CS.seek 0ml @15:36:15.91 Flushing lower manifold's waste2b port @15:36:16.03 CSV.dial :flush @15:36:16.48 CS.reconfigure :fast @15:36:16.53 CS.seek 5ml @15:36:29.32 delay 5 seconds @15:36:34.33 CSV.dial :air @15:36:34.83 CS.seek 7ml @15:36:40.44 CSV.dial CTV @15:36:40.48
CTV.dial CSV @15:36:41.85 CBV.dial :waste2b @15:36:42.03 CS.seek 0ml @15:36:58.97 Disinfecting external Intake with kill @15:36:59.10 CSV.dial CSR @15:36:59.53 Pulling 8ml of CSR.kill @15:36:59.54 CSR.select :kill @15:36:59.66 CS.seek 8ml @15:37:19.57 delay 5 seconds @15:37:24.58 CSR.select :air @15:37:24.70 CS.seek 10ml @15:37:30.28 CSV.dial CBV @15:37:30.31
CBV.dial CSV @15:37:31.38 CTV.dial :intake @15:37:32.14 Intake.select :open @15:37:32.25 CS.seek 2ml @15:37:51.78 Intake.select :close @15:37:51.89 CTV.dial :air @15:37:52.63 CS.reconfigure :default @15:37:52.68 CS.seek 0ml @15:37:58.63 CSV.dial CSR @15:37:59.64 Pulling 5ml of CSR.flush @15:37:59.65 CSR.select :flush @15:37:59.77 CS.seek 5ml @15:38:13.35 delay 5 seconds @15:38:18.36 CSR.select :air @15:38:18.48 CS.seek 10ml @15:38:32.28 CSV.dial CBV @15:38:33.29 CS.seek 0ml @15:38:59.88 Garaging FlushPuck in Collection Clamp @15:38:59.95 Elbow.reconfigure :tight @15:38:59.99 Elbow.seek CC @15:39:00.25 Elbow.reconfigure :loose @15:39:00.35 Forearm.seek CC @15:39:01.38 Hand.close @15:39:01.93 CC.seek :open @15:39:06.59 Forearm.seek :retract @15:39:07.59 Elbow.reconfigure :tight @15:39:07.64 Elbow.seek :garage @15:39:10.56 Elbow.reconfigure :loose @15:39:10.66 Forearm.reconfigure :tight @15:39:10.71 Forearm.seek :garage @15:39:11.75 Hand.open @15:39:12.37 Forearm.reconfigure :loose @15:39:12.41 Forearm.seek :retract @15:39:13.37 Completed [initialPurge; standAloneWCR([nil,100], $da)] begun 15:11:42 @15:39:13.42 Asleep after completing standAloneWCR_010224 assays @15:39:13.44 GoodNews.email "{1 => 1,\n 2 => 0}\nCTD data unavailable\nCan@15:30:56, 26% flow, 22.3C, 16% humidity, 15.1psia, 13.656V, 0.472A, 0.646Ah, 6.44W\nSampled 100.0ml",Subject: "Asleep after completing standAloneWCR_010224 assays" @15:39:17.77 upsync + ESPwhich standAloneWCR_10224.rb + fn=standAloneWCR_10224.rb + IFS=: + set /home/esp/esp2/mission . + fullPath=/home/esp/esp2/mission/standAloneWCR_10224.rb + [ -f /home/esp/esp2/mission/standAloneWCR_10224.rb ] + fullPath=./standAloneWCR_10224.rb + [ -f ./standAloneWCR_10224.rb ] + return 1 + scriptPath= + [ standAloneWCR_10224.rb = standAloneWCR_10224 ] + echo No 'standAloneWCR_10224.rb' script found on ESPpath: No 'standAloneWCR_10224.rb' script found on ESPpath: + echo /home/esp/esp2/mission:. /home/esp/esp2/mission:. + exit 1 + ESPwhich standAloneWCR_010224.rb + fn=standAloneWCR_010224.rb + IFS=: + set /home/esp/esp2/mission . + fullPath=/home/esp/esp2/mission/standAloneWCR_010224.rb + [ -f /home/esp/esp2/mission/standAloneWCR_010224.rb ] + realpath /home/esp/esp2/mission/standAloneWCR_010224.rb + return 0 + scriptPath=/home/esp/esp2local/mission/standAloneWCR_010224.rb + exec ruby /home/esp/esp2/lib/esp.rb /home/esp/esp2local/mission/standAloneWCR_010224.rb @14:23:50.29UTC09-Jan-24
ESP2 v3.42 I2C Gateway v3.20 for core bus @14:23:52.94 Configuration for short puck jake 1/11/21 by brent@mbari.org /home/esp/esp2/type/shallow/jake/configure.rb @14:23:56.55 Polling Can every 10:00 @14:23:56.61 Loading /home/esp/esp2local/mission/standAloneWCR_010224.rb mission @14:23:56.66 Preparing to sleep until + 00:00:05 @14:23:56.70 GoodNews.email "Starting standAloneWCR_010224 w/pucks in tube 2\n{2 => 1}\nCTD data unavailable\nCan@14:23:56, 26% flow, 21.1C, 18% humidity, 15.1psia, 13.710V, 0.345A, 6.557Ah, 4.73W",Subject: "standAloneWCR_010224 begun" @14:23:56.72
Asleep til + 00:00:05 @14:23:56.77 Continuing at 14:24 @14:23:57.00 Gate.power :core,:OFF @14:23:57.01 Gate.power :raw,:OFF @14:23:57.02 Shutdown @14:24:00.32 GoodNews.email "Then, in tube 2: initialPurge; standAloneWCR([nil,100], $da)\n/home/esp/esp2local/mission/standAloneWCR_010224.rb:10\n{2 => 1}\nCTD data unavailable\nCan@14:23:56, 26% flow, 21.1C, 18% humidity, 15.1psia, 13.710V, 0.315A, 6.557Ah, 4.31W",Subject: "Asleep until 14:24" @14:24:01.65
Powering core bus @14:24:01.69 upsync @14:24:04.57 upsync @14:24:07.26
Gate.power :raw,:ON @14:24:08.63 CS.configure :default @14:24:08.68 PS.configure :default @14:24:08.73 SS.configure :default @14:24:08.77 CC.configure :default @14:24:08.82 PC.configure :default @14:24:08.89 Forearm.configure :loose @14:24:08.93 Elbow.configure :loose @14:24:09.03 SC.configure :loose @14:24:09.07 SE.configure :default @14:24:09.12 CH.configure :default @14:24:09.16 PH.configure :default @14:24:09.19 All dwarf controllers running firmware version 3.85 @14:24:09.30 Valve.reset @14:24:09.55 Intake.select :close @14:24:09.62 Exhaust.select :close @14:24:09.64 CTV.dial :air @14:24:09.71 PSV.dial :air @14:24:09.77 CSV.dial :waste1 @14:24:09.92
Forearm.home.seek :retract @14:24:09.94 PS.home.seek 0ml @14:24:10.28 SSV.dial :spit,avoiding: :exhaust @14:24:10.71 Hand.close @14:24:10.74
Elbow.reconfigure :loose @14:24:10.84 Elbow.jog 1500 @14:24:11.13 CS.reconfigure :fast @14:24:11.16 SS.home.seek 0ml @14:24:11.26 CS.home.seek 0ml @14:24:11.33
Elbow.reconfigure :loose @14:24:11.39 Elbow.home.seek :clear @14:24:14.66 SE.home.seek :down @14:24:15.27 SC.reconfigure :tight @14:24:15.32 SC.home.seek :initial @14:24:18.24 PS.stop @14:24:40.46
SC.configure :limp @14:24:40.51 Forearm.seek :retract @14:24:40.58 Elbow.seek :hammer @14:24:41.58 Forearm.reconfigure :tight @14:24:41.63 Forearm.seek retracted @14:24:42.00 PC.home.seek :open @14:24:42.02
CC.home.seek :open @14:24:45.49 Phase [initialPurge; standAloneWCR([nil,100], $da)] @14:24:45.51 Notice.email "At 14:24:45 began in tube 2: initialPurge; standAloneWCR([nil,100], $da)\n/home/esp/esp2local/mission/standAloneWCR_010224.rb:10\n{2 => 1}\nCTD data unavailable\nCan@14:24:45, 26% flow, 21.1C, 18% humidity, 15.1psia, 13.773V, 0.296A, 6.562Ah, 4.08W",Subject: "Started standAloneWCR_010224 phase initialPurge; standAloneWCR([nil,100], $da)" @14:24:45.52
Starting initialPurge @14:24:45.55 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:24:45.62 Hand.open @14:24:46.35 Elbow.reconfigure :tight @14:24:46.40 Elbow.seek FlushPuck::Garage @14:24:48.24 Elbow.reconfigure :loose @14:24:48.35 Forearm.reconfigure :loose @14:24:48.40 Forearm.seek :garage @14:24:49.35 Hand.close @14:24:49.99 upsync @14:24:50.14
Forearm.seek :retract @14:24:51.14 Elbow.reconfigure :tight @14:24:51.19 Elbow.seek CC @14:24:54.05 Elbow.reconfigure :loose @14:24:54.16 Forearm.reconfigure :tight @14:24:54.20 Forearm.seek CC @14:24:55.42 CC.seek :closed @14:25:00.01 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.33 @14:25:00.08 Hand.open @14:25:00.77 Forearm.reconfigure :loose @14:25:00.82 Forearm.seek :retract @14:25:01.78 Flushing the Collection Valve::Manifold @14:25:01.90 CSV.dial CSR @14:25:03.18 Pulling 5ml of CSR.flush @14:25:03.19 CSR.select :flush @14:25:03.30 CS.reconfigure :default @14:25:03.35 CS.seek 5ml @14:25:16.91 delay 5 seconds @14:25:22.28 CSR.select :air @14:25:22.51 CS.reconfigure :fast @14:25:22.56 CS.seek 7.499ml @14:25:29.38 CSV.dial :waste @14:25:30.67 CS.reconfigure :default @14:25:30.71 CS.seek 0ml @14:25:50.72 Purging external Intake @14:25:50.89 CSV.dial :flush @14:25:51.34 CS.reconfigure :fast @14:25:51.39 CS.seek 10ml @14:26:16.19 delay 5 seconds @14:26:21.22 CSV.dial CBV @14:26:21.26
CBV.dial CSV @14:26:21.97 CTV.dial :intake @14:26:22.75 Intake.select :open @14:26:22.86 CS.seek 0ml @14:26:46.60 Intake.select :close @14:26:46.70 CTV.dial :air @14:26:47.48 Flushing upper manifold @14:26:47.60 CSV.dial :flush @14:26:48.34 CS.seek 5ml @14:27:01.35 delay 5 seconds @14:27:06.37 CSV.dial :air @14:27:06.87 CS.seek 7ml @14:27:12.38 CSV.dial CBV @14:27:12.41
CBV.dial CSV @14:27:13.43 CTV.dial :air @14:27:13.51 CS.seek 0ml @14:27:30.13 delay 7 seconds @14:27:37.16 CS.seek 3ml @14:27:45.22 CSV.dial :waste @14:27:45.68 CS.seek 0ml @14:27:53.55 CSV.dial :flush @14:27:53.99 CS.seek 3ml @14:28:02.10 delay 5 seconds @14:28:07.12 CSV.dial :air @14:28:07.74 CS.seek 5ml @14:28:13.28 CSV.dial :air @14:28:13.37 CS.seek 0ml @14:28:25.56 delay 7 seconds @14:28:32.59 CS.seek 3ml @14:28:40.72 CSV.dial :waste @14:28:41.45 CS.seek 0ml @14:28:49.19 Garaging FlushPuck in Collection Clamp @14:28:49.27 Elbow.reconfigure :tight @14:28:49.31 Elbow.seek CC @14:28:49.73 Elbow.reconfigure :loose @14:28:49.83 Forearm.seek CC @14:28:50.82 Hand.close @14:28:51.46 CC.seek :open @14:28:56.49 Forearm.seek :retract @14:28:57.45 Elbow.reconfigure :tight @14:28:57.49 Elbow.seek :garage @14:29:00.26 Elbow.reconfigure :loose @14:29:00.36 Forearm.reconfigure :tight @14:29:00.41 Forearm.seek :garage @14:29:01.35 Hand.open @14:29:02.02 Forearm.reconfigure :loose @14:29:02.06 Forearm.seek :retract @14:29:02.99 Starting stand-alone da WCR @14:29:03.00 da wcr sampling at most 100ml @14:29:03.03 Whole Cell Archive sampling 100ml @14:29:03.05 DAfans.email "Started stand-alone da WCR in tube 2 at 14:29:03UTC09-Jan-24\n{2 => 1}\nCTD data unavailable\nCan@14:24:45, 26% flow, 21.1C, 18% humidity, 15.1psia, 13.773V, 0.296A, 6.562Ah, 4.08W",Subject: "da wcr sampling at most 100ml" @14:29:03.06 Loading wcr puck from tube 2 into Collection Clamp @14:29:03.34 SC.reconfigure :tight @14:29:03.41 SC.seek 2 @14:29:07.50 upsync @14:29:10.16 SC.configure :limp @14:29:10.40 Elbow.reconfigure :tight @14:29:10.45 Elbow.seek 2 @14:29:12.36 Forearm.reconfigure :tight @14:29:12.41 Forearm.seek 2 + 450 counts @14:29:13.44 SE.reconfigure :pick @14:29:13.51 SE.seek :beamBreak @14:29:16.55 SE.configure :default @14:29:16.72 Elbow.seek 2 @14:29:16.91 Forearm.seek 2 + 300 counts @14:29:17.19 Forearm.reconfigure :loose @14:29:17.25 Hand.close @14:29:17.85 SE.seek :down @14:29:18.43 Forearm.seek :retract @14:29:19.46 Elbow.seek CC @14:29:20.64 SE.stop @14:29:21.03 Elbow.reconfigure :loose @14:29:21.14 Forearm.reconfigure :tight @14:29:21.19 Forearm.seek CC @14:29:22.53 CC.seek :closed @14:29:27.07 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.27 @14:29:27.08 Hand.open @14:29:27.77 Forearm.reconfigure :loose @14:29:27.82 Forearm.seek :retract @14:29:28.80 collectSample 100ml w/bubble point of 35psi @14:29:28.82 Priming 7ml @14:29:28.83 Intake.select :close @14:29:28.84 Exhaust.select :close @14:29:28.85 SS.hold @14:29:29.06 Calibrating pressure and temperature sensors @14:29:29.09 CTV.dial :air @14:29:29.18 SSV.dial :spit,avoiding: :exhaust @14:29:29.26 SS.reconfigure :fast @14:29:29.31 SS.seek 5ml @14:29:32.41 SSV.dial CBV @14:29:32.43 CBV.dial SSV @14:29:33.81 delay 3.8 seconds @14:29:37.74 Sensor limits: Intake max 110.8psia; Outlet max 104.5psia @14:29:37.75 SSV.dial :spit @14:29:39.05 SS.seek 0ml @14:29:41.70 SSV.dial CBV @14:29:43.03 CTV.dial :intake @14:29:43.82 SSV.dial CBV @14:29:43.84 CBV.dial SSV @14:29:44.07 Allowing 40s for 7.0ml of remaining 7.0ml @14:29:44.08 Intake.select :open @14:29:46.24 Intake @15.6psia, vacuum from 10.0 to 13.0psi @14:29:46.26 SS.reconfigure :inhaling 2.20ml/s @14:29:46.31 SS.seek 7ml @14:29:53.33 Stroke completed -- waiting for pressure to equalize ... @14:29:53.34 SS.reconfigure :exhale @14:29:53.39 SS.hold @14:29:57.21 Intake.select :close @14:29:57.37 SSV.dial :exhaust @14:29:58.14 Exhaust.select :open @14:29:58.25 SS.seek 0ml @14:30:02.28 Exhaust.select :close @14:30:02.39 Sampling 100.0ml @14:30:02.41 Intake.select :close @14:30:02.42 Exhaust.select :close @14:30:02.43 SS.reconfigure :default @14:30:02.47 SS.hold @14:30:02.67 CTV.dial :intake @14:30:02.85 SSV.dial CBV @14:30:02.87 CBV.dial SSV @14:30:03.62 Allowing 130s for 25.0ml of remaining 100.0ml @14:30:03.64 Intake.select :open @14:30:05.80 Intake @15.8psia, vacuum from 10.0 to 13.0psi @14:30:05.82 SS.reconfigure :inhaling 2.20ml/s @14:30:05.87 SS.seek 25ml @14:30:20.00 Vacuum too high -- waiting up to 40 seconds @14:30:20.02 SS.reconfigure :exhale @14:30:20.07 SS.hold @14:30:20.73 Intake @12.0psia, vacuum from 6.5 to 9.5psi @14:30:20.75 SS.reconfigure :inhaling 1.76ml/s @14:30:20.80 SS.seek 25ml @14:30:22.18 Stroke completed -- waiting for pressure to equalize ... @14:30:22.20 SS.reconfigure :exhale @14:30:22.24 SS.hold @14:30:26.46 Intake.select :close @14:30:26.62 SSV.dial :exhaust @14:30:27.38 Exhaust.select :open @14:30:27.49 SS.seek 0ml @14:30:36.51 Exhaust.select :close @14:30:36.63 SSV.dial CBV @14:30:36.65 CBV.dial SSV @14:30:37.41 Allowing 130s for 25.0ml of remaining 75.0ml @14:30:37.42 Intake.select :open @14:30:39.58 Intake @15.9psia, vacuum from 10.0 to 13.0psi @14:30:39.60 SS.reconfigure :inhaling 1.76ml/s @14:30:39.65 SS.seek 25ml @14:30:57.01 Stroke completed -- waiting for pressure to equalize ... @14:30:57.02 SS.reconfigure :exhale @14:30:57.07 SS.hold @14:31:01.81 Intake.select :close @14:31:01.97 SSV.dial :exhaust @14:31:02.72 Exhaust.select :open @14:31:02.83 SS.seek 0ml @14:31:11.76 Exhaust.select :close @14:31:11.87 SSV.dial CBV @14:31:11.89 CBV.dial SSV @14:31:12.65 Allowing 130s for 25.0ml of remaining 50.0ml @14:31:12.67 Intake.select :open @14:31:14.83 Intake @15.9psia, vacuum from 10.0 to 13.0psi @14:31:14.85 SS.reconfigure :inhaling 1.76ml/s @14:31:14.90 SS.seek 25ml @14:31:32.84 Stroke completed -- waiting for pressure to equalize ... @14:31:32.88 SS.reconfigure :exhale @14:31:32.95 SS.hold @14:31:39.00 Intake.select :close @14:31:39.16 SSV.dial :exhaust @14:31:39.89 Exhaust.select :open @14:31:40.00 SS.seek 0ml @14:31:48.96 Exhaust.select :close @14:31:49.08 SSV.dial CBV @14:31:49.10 CBV.dial SSV @14:31:49.84 Allowing 130s for 25.0ml of remaining 25.0ml @14:31:49.86 Intake.select :open @14:31:52.01 Intake @15.9psia, vacuum from 10.0 to 13.0psi @14:31:52.03 SS.reconfigure :inhaling 1.76ml/s @14:31:52.08 SS.seek 25ml @14:32:08.81 Vacuum too high -- waiting up to 40 seconds @14:32:08.83 SS.reconfigure :exhale @14:32:08.88 SS.hold @14:32:09.46 Intake @12.9psia, vacuum from 7.4 to 10.4psi @14:32:09.48 SS.reconfigure :inhaling 1.41ml/s @14:32:09.53 SS.seek 25ml @14:32:11.29 Stroke completed -- waiting for pressure to equalize ... @14:32:11.30 SS.reconfigure :exhale @14:32:11.35 SS.hold @14:32:16.88 Intake.select :close @14:32:17.47 SSV.dial :exhaust @14:32:18.23 Exhaust.select :open @14:32:18.34 SS.seek 0ml @14:32:27.25 Exhaust.select :close @14:32:27.36 Sampled 100.0ml @14:32:27.38 Drying puck before fixing for 5:00 @14:32:27.39 CBV.dial :waste2b @14:32:28.12 delay 1:00 @14:33:28.15 CSV.dial :air @14:33:28.90 CS.seek 10ml @14:33:53.62 Pumping wcr puck from tube 2 up to 35psi... @14:33:53.64 CBV.dial SSV @14:33:54.89 CBV.dial between CSV and waste2b @14:33:55.85 CSV.dial between air and CTV @14:33:56.16 CS.seek 7.35ml @14:34:03.06 CTV.dial CSV,avoiding: :air @14:34:03.09 CSV.dial CTV @14:34:03.92 CS.reconfigure :pressurize @14:34:03.98 CS.seek 0ml @14:34:18.70 CBV.dial :waste2b @14:34:19.03 CS.reconfigure :default @14:34:19.08 CS.seek 0ml @14:34:24.37 delay 1:00 @14:35:24.40 CTV.dial :air,avoiding: :intake @14:35:25.70 Fix #1 of 2 (with RNAlater) for 2:30 @14:35:25.72 CSV.dial CSR @14:35:26.17 Pulling 2ml of CSR.RNAlater @14:35:26.18 CSR.select :RNAlater @14:35:26.30 CS.seek 2ml @14:35:32.21 delay 5 seconds @14:35:37.23 CSR.select :air @14:35:37.35 CS.reconfigure :fast @14:35:37.40 CS.seek 10ml @14:35:57.19 Pumping wcr puck from tube 2 up to 35psi... @14:35:57.20 CBV.dial SSV @14:35:58.45 CBV.dial between CSV and waste2b @14:35:59.41 CSV.dial between air and CTV @14:36:00.02 CS.seek 7.299ml @14:36:07.06 CTV.dial CSV,avoiding: :air @14:36:07.09 CSV.dial CTV @14:36:08.44 CS.reconfigure :pressurize @14:36:08.49 CS.seek 0ml @14:36:21.92 delay 2:30 @14:38:51.95 CBV.dial :waste2b @14:38:52.28 CS.reconfigure :default @14:38:52.33 CS.seek 0ml @14:38:58.96 delay 1:00 @14:39:58.99 CTV.dial :air,avoiding: :intake @14:40:00.29 Fix #2 of 2 (with RNAlater) for 2:30 @14:40:00.31 CSV.dial CSR @14:40:00.81 Pulling 2ml of CSR.RNAlater @14:40:00.82 CSR.select :RNAlater @14:40:00.94 CS.seek 2ml @14:40:06.87 delay 5 seconds @14:40:11.89 CSR.select :air @14:40:12.01 CS.reconfigure :fast @14:40:12.06 CS.seek 10ml @14:40:31.84 Pumping wcr puck from tube 2 up to 35psi... @14:40:31.85 CBV.dial SSV @14:40:33.11 CBV.dial between CSV and waste2b @14:40:34.05 CSV.dial between air and CTV @14:40:34.65 CS.seek 7.249ml @14:40:41.99 CTV.dial CSV,avoiding: :air @14:40:42.02 CSV.dial CTV @14:40:43.38 CS.reconfigure :pressurize @14:40:43.44 CS.seek 0ml @14:41:02.04 delay 2:30 @14:43:32.07 CBV.dial :waste2b @14:43:32.40 CS.reconfigure :default @14:43:32.45 CS.seek 0ml @14:43:33.74 delay 1:00 @14:44:33.77 CTV.dial :air,avoiding: :intake @14:44:35.11 Storing wcr puck in Collection Clamp to tube 1 @14:44:35.28 SC.reconfigure :tight @14:44:35.33 SC.seek 1 @14:44:38.90 SC.configure :limp @14:44:39.12 Elbow.reconfigure :tight @14:44:39.17 Elbow.seek 1 @14:44:40.96 Forearm.reconfigure :tight @14:44:41.01 Forearm.seek 1 + 450 counts @14:44:42.08 SE.reconfigure :pick @14:44:42.14 SE.seek :beamBreak @14:44:44.99 SE.configure :default @14:44:45.04 SE.seek :place @14:44:45.64 Forearm.reconfigure :loose @14:44:45.69 Forearm.seek :retract @14:44:46.70 Elbow.seek CC @14:44:48.38 Elbow.reconfigure :loose @14:44:48.92 Forearm.seek CC @14:44:49.90 Hand.close @14:44:50.58 CC.seek :open @14:44:55.31 Forearm.seek :retract @14:44:56.26 Elbow.reconfigure :tight @14:44:56.31 Elbow.seek 1 @14:44:58.06 Elbow.reconfigure :limp @14:44:58.11 Forearm.seek 1 @14:44:59.10 Elbow.hold @14:44:59.17 Elbow.reconfigure :tight @14:44:59.22 Hand.open @14:44:59.94 Forearm.force -20 @14:44:59.98 Forearm.seek 1 + 301 counts @14:45:00.27 Forearm.reconfigure :tight @14:45:00.32 Forearm.seek 1 + 450 counts @14:45:00.57 SE.seek :down @14:45:01.71 Forearm.reconfigure :loose @14:45:01.76 Forearm.seek :retract @14:45:03.26 SE.stop @14:45:03.30
Starting finalPurge @14:45:03.32 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:45:03.50 Elbow.seek FlushPuck::Garage @14:45:05.25 Elbow.reconfigure :loose @14:45:05.35 Forearm.seek :garage @14:45:06.40 Hand.close @14:45:07.19 Forearm.seek :retract @14:45:08.13 Elbow.reconfigure :tight @14:45:08.18 Elbow.seek CC @14:45:11.46 Elbow.reconfigure :loose @14:45:11.63 Forearm.reconfigure :tight @14:45:11.67 Forearm.seek CC @14:45:12.87 CC.seek :closed @14:45:17.40 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.29 @14:45:17.46 Hand.open @14:45:18.13 Forearm.reconfigure :loose @14:45:18.18 Forearm.seek :retract @14:45:19.15 Flushing the Collection Valve::Manifold @14:45:19.27 CSV.dial CSR @14:45:19.72 Pulling 4.999ml of CSR.flush @14:45:19.73 CSR.select :flush @14:45:19.84 CS.seek 5ml @14:45:33.64 delay 5 seconds @14:45:38.66 CSR.select :air @14:45:38.83 CS.reconfigure :fast @14:45:38.88 CS.seek 7.5ml @14:45:45.76 CSV.dial :waste @14:45:47.05 CS.reconfigure :default @14:45:47.10 CS.seek 0ml @14:46:07.17 Flushing lower manifold's waste2b port @14:46:07.30 CSV.dial :flush @14:46:07.74 CS.reconfigure :fast @14:46:07.79 CS.seek 5ml @14:46:20.61 delay 5 seconds @14:46:25.63 CSV.dial :air @14:46:26.14 CS.seek 7ml @14:46:31.70 CSV.dial CTV @14:46:31.73
CTV.dial CSV @14:46:33.11 CBV.dial :waste2b @14:46:33.29 CS.seek 0ml @14:46:50.24 Disinfecting external Intake with kill @14:46:50.35 CSV.dial CSR @14:46:50.81 Pulling 8ml of CSR.kill @14:46:50.82 CSR.select :kill @14:46:50.94 CS.seek 8ml @14:47:10.86 delay 5 seconds @14:47:15.88 CSR.select :air @14:47:16.00 CS.seek 10ml @14:47:22.04 CSV.dial CBV @14:47:22.06
CBV.dial CSV @14:47:23.14 CTV.dial :intake @14:47:23.90 Intake.select :open @14:47:24.01 CS.seek 2ml @14:47:42.89 Intake.select :close @14:47:42.99 CTV.dial :air @14:47:43.75 CS.reconfigure :default @14:47:43.80 CS.seek 0ml @14:47:49.70 CSV.dial CSR @14:47:50.72 Pulling 5ml of CSR.flush @14:47:50.73 CSR.select :flush @14:47:50.84 CS.seek 5ml @14:48:04.48 delay 5 seconds @14:48:09.50 CSR.select :air @14:48:09.62 CS.seek 10ml @14:48:23.17 CSV.dial CBV @14:48:24.18 CS.seek 0ml @14:48:50.55 Garaging FlushPuck in Collection Clamp @14:48:50.62 Elbow.reconfigure :tight @14:48:50.66 Elbow.seek CC @14:48:51.06 Elbow.reconfigure :loose @14:48:51.16 Forearm.seek CC @14:48:52.16 Hand.close @14:48:52.82 CC.seek :open @14:48:57.44 Forearm.seek :retract @14:48:58.43 Elbow.reconfigure :tight @14:48:58.47 Elbow.seek :garage @14:49:01.31 Elbow.reconfigure :loose @14:49:01.41 Forearm.reconfigure :tight @14:49:01.45 Forearm.seek :garage @14:49:02.42 Hand.open @14:49:03.13 Forearm.reconfigure :loose @14:49:03.18 Forearm.seek :retract @14:49:04.13 Completed [initialPurge; standAloneWCR([nil,100], $da)] begun 14:24:45 @14:49:04.18 Asleep after completing standAloneWCR_010224 assays @14:49:04.19 GoodNews.email "{1 => 1,\n 2 => 0}\nCTD data unavailable\nCan@14:43:59, 26% flow, 21.2C, 18% humidity, 15.1psia, 13.661V, 0.447A, 6.707Ah, 6.11W\nSampled 100.0ml",Subject: "Asleep after completing standAloneWCR_010224 assays" @14:49:08.62 upsync + ESPwhich standAloneWCR_010324.rb + fn=standAloneWCR_010324.rb + IFS=: + set /home/esp/esp2/mission . + fullPath=/home/esp/esp2/mission/standAloneWCR_010324.rb + [ -f /home/esp/esp2/mission/standAloneWCR_010324.rb ] + fullPath=./standAloneWCR_010324.rb + [ -f ./standAloneWCR_010324.rb ] + return 1 + scriptPath= + [ standAloneWCR_010324.rb = standAloneWCR_010324 ] + echo No 'standAloneWCR_010324.rb' script found on ESPpath: No 'standAloneWCR_010324.rb' script found on ESPpath: + echo /home/esp/esp2/mission:. /home/esp/esp2/mission:. + exit 1 + ESPwhich standAloneWCR_010224.rb + fn=standAloneWCR_010224.rb + IFS=: + set /home/esp/esp2/mission . + fullPath=/home/esp/esp2/mission/standAloneWCR_010224.rb + [ -f /home/esp/esp2/mission/standAloneWCR_010224.rb ] + realpath /home/esp/esp2/mission/standAloneWCR_010224.rb + return 0 + scriptPath=/home/esp/esp2local/mission/standAloneWCR_010224.rb + exec ruby /home/esp/esp2/lib/esp.rb /home/esp/esp2local/mission/standAloneWCR_010224.rb @19:49:40.38UTC09-Jan-24
ESP2 v3.42 I2C Gateway v3.20 for core bus @19:49:43.21 Configuration for short puck jake 1/11/21 by brent@mbari.org /home/esp/esp2/type/shallow/jake/configure.rb @19:49:46.94 Polling Can every 10:00 @19:49:46.95 Cleanly exited at 19:48:54UTC09-Jan-24 @19:49:47.00 Loading /home/esp/esp2local/mission/standAloneWCR_010224.rb mission @19:49:47.02 RuntimeError in MAIN -- Mission's startTube should be 3, not 2 @19:49:47.05 Waiting to Resume from Checkpoint "Loading /home/esp/esp2local/mission/standAloneWCR_010224.rb mission" @19:49:47.06 BadNews.email "Mission's startTube should be 3, not 2",Subject: "RuntimeError in MAIN" @19:49:51.35 upsync + ESPwhich standAloneWCR_010224.rb + fn=standAloneWCR_010224.rb + IFS=: + set /home/esp/esp2/mission . + fullPath=/home/esp/esp2/mission/standAloneWCR_010224.rb + [ -f /home/esp/esp2/mission/standAloneWCR_010224.rb ] + realpath /home/esp/esp2/mission/standAloneWCR_010224.rb + return 0 + scriptPath=/home/esp/esp2local/mission/standAloneWCR_010224.rb + exec ruby /home/esp/esp2/lib/esp.rb /home/esp/esp2local/mission/standAloneWCR_010224.rb Log::Locked in trapHandler -- Another process is already writing to /var/log/esp/real.log + ESPwhich standAloneWCR_010224.rb + fn=standAloneWCR_010224.rb + IFS=: + set /home/esp/esp2/mission . + fullPath=/home/esp/esp2/mission/standAloneWCR_010224.rb + [ -f /home/esp/esp2/mission/standAloneWCR_010224.rb ] + realpath /home/esp/esp2/mission/standAloneWCR_010224.rb + return 0 + scriptPath=/home/esp/esp2local/mission/standAloneWCR_010224.rb + exec ruby /home/esp/esp2/lib/esp.rb /home/esp/esp2local/mission/standAloneWCR_010224.rb @19:52:41.79UTC09-Jan-24
ESP2 v3.42 I2C Gateway v3.20 for core bus @19:52:43.47 Errno::ESRCH in MAIN -- No such process -- removing stale lock on /dev/ctd @19:52:44.44 Configuration for short puck jake 1/11/21 by brent@mbari.org /home/esp/esp2/type/shallow/jake/configure.rb @19:52:47.96 Polling Can every 10:00 @19:52:48.02 Loading /home/esp/esp2local/mission/standAloneWCR_010224.rb mission @19:52:48.08 Preparing to sleep until + 00:00:05 @19:52:48.10 GoodNews.email "Starting standAloneWCR_010224 w/pucks in tube 2\n{2 => 1}\nCTD data unavailable\nCan@19:52:47, 26% flow, 20.9C, 22% humidity, 15.0psia, 13.812V, 0.315A, 8.251Ah, 4.34W",Subject: "standAloneWCR_010224 begun" @19:52:48.16
Asleep til + 00:00:05 @19:52:48.17 Continuing at 19:52 @19:52:48.46 Gate.power :core,:OFF @19:52:48.48 Gate.power :raw,:OFF Shutdown @19:52:51.21 GoodNews.email "Then, in tube 2: initialPurge; standAloneWCR([nil,100], $da)\n/home/esp/esp2local/mission/standAloneWCR_010224.rb:10\n{2 => 1}\nCTD data unavailable\nCan@19:52:48, 26% flow, 20.9C, 22% humidity, 15.0psia, 13.812V, 0.315A, 8.251Ah, 4.34W",Subject: "Asleep until 19:52" @19:52:52.56 upsync @19:52:53.06
Powering core bus @19:52:55.37 upsync @19:52:58.23
Gate.power :raw,:ON @19:52:59.54 CS.configure :default @19:52:59.59 PS.configure :default @19:52:59.64 SS.configure :default @19:52:59.69 CC.configure :default @19:52:59.73 PC.configure :default @19:52:59.80 Forearm.configure :loose @19:52:59.85 Elbow.configure :loose @19:52:59.92 SC.configure :loose @19:52:59.96 SE.configure :default @19:53:00.01 CH.configure :default @19:53:00.05 PH.configure :default @19:53:00.08 All dwarf controllers running firmware version 3.85 @19:53:00.19 Valve.reset @19:53:00.46 Intake.select :close @19:53:00.49 Exhaust.select :close @19:53:00.51 CTV.dial :air @19:53:00.61 PSV.dial :air @19:53:00.64 CSV.dial :waste1 @19:53:00.81
Forearm.home.seek :retract @19:53:00.83 PS.home.seek 0ml @19:53:01.15 SSV.dial :spit,avoiding: :exhaust @19:53:01.60 Hand.close @19:53:01.64
Elbow.reconfigure :loose @19:53:01.72 Elbow.jog 1500 @19:53:02.00 CS.reconfigure :fast @19:53:02.03 SS.home.seek 0ml @19:53:02.12 CS.home.seek 0ml @19:53:02.27
Elbow.reconfigure :loose @19:53:02.31 Elbow.home.seek :clear @19:53:05.52 SE.home.seek :down @19:53:06.15 SC.reconfigure :tight @19:53:06.20 SC.home.seek :initial @19:53:09.08 PS.stop @19:53:31.36
SC.configure :limp @19:53:31.41 Forearm.seek :retract @19:53:31.48 Elbow.seek :hammer @19:53:32.60 Forearm.reconfigure :tight @19:53:32.65 Forearm.seek retracted @19:53:33.03 CC.home.seek :open @19:53:33.05 PC.home.seek :open @19:53:36.58
Phase [initialPurge; standAloneWCR([nil,100], $da)] @19:53:36.59 Notice.email "At 19:53:36 began in tube 2: initialPurge; standAloneWCR([nil,100], $da)\n/home/esp/esp2local/mission/standAloneWCR_010224.rb:10\n{2 => 1}\nCTD data unavailable\nCan@19:53:36, 26% flow, 20.9C, 23% humidity, 15.0psia, 13.769V, 0.278A, 8.256Ah, 3.83W",Subject: "Started standAloneWCR_010224 phase initialPurge; standAloneWCR([nil,100], $da)" @19:53:36.62
Starting initialPurge @19:53:36.64 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @19:53:36.71 Hand.open @19:53:37.49 Elbow.reconfigure :tight @19:53:37.53 Elbow.seek FlushPuck::Garage @19:53:39.33 Elbow.reconfigure :loose @19:53:39.43 Forearm.reconfigure :loose @19:53:39.47 Forearm.seek :garage @19:53:40.49 Hand.close @19:53:41.02 upsync @19:53:41.24
Forearm.seek :retract @19:53:42.28 Elbow.reconfigure :tight @19:53:42.32 Elbow.seek CC @19:53:45.28 Elbow.reconfigure :loose @19:53:45.39 Forearm.reconfigure :tight @19:53:45.43 Forearm.seek CC @19:53:46.73 CC.seek :closed @19:53:51.32 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.36 @19:53:51.38 Hand.open @19:53:52.05 Forearm.reconfigure :loose @19:53:52.10 Forearm.seek :retract @19:53:53.07 Flushing the Collection Valve::Manifold @19:53:53.19 CSV.dial CSR @19:53:54.58 Pulling 5ml of CSR.flush @19:53:54.59 CSR.select :flush @19:53:54.71 CS.reconfigure :default @19:53:54.76 CS.seek 5ml @19:54:08.35 delay 5 seconds @19:54:13.37 CSR.select :air @19:54:13.94 CS.reconfigure :fast @19:54:13.99 CS.seek 7.5ml @19:54:20.75 CSV.dial :waste @19:54:22.02 CS.reconfigure :default @19:54:22.07 CS.seek 0ml @19:54:42.10 Purging external Intake @19:54:42.22 CSV.dial :flush @19:54:42.68 CS.reconfigure :fast @19:54:42.73 CS.seek 10ml @19:55:07.13 delay 5 seconds @19:55:12.15 CSV.dial CBV @19:55:12.19
CBV.dial CSV @19:55:12.92 CTV.dial :intake @19:55:13.70 Intake.select :open @19:55:13.81 CS.seek 0ml @19:55:37.22 Intake.select :close @19:55:37.32 CTV.dial :air @19:55:38.10 Flushing upper manifold @19:55:38.22 CSV.dial :flush @19:55:38.95 CS.seek 5ml @19:55:51.91 delay 5 seconds @19:55:56.93 CSV.dial :air @19:55:57.42 CS.seek 7ml @19:56:02.97 CSV.dial CBV @19:56:03.00
CBV.dial CSV @19:56:04.02 CTV.dial :air @19:56:04.10 CS.seek 0ml @19:56:20.63 delay 7 seconds @19:56:27.65 CS.seek 3ml @19:56:35.72 CSV.dial :waste @19:56:36.18 CS.seek 0ml @19:56:43.92 CSV.dial :flush @19:56:44.37 CS.seek 3ml @19:56:52.66 delay 5 seconds @19:56:57.67 CSV.dial :air @19:56:58.17 CS.seek 5ml @19:57:03.72 CSV.dial :air @19:57:03.90 CS.seek 0ml @19:57:16.06 delay 7 seconds @19:57:23.09 CS.seek 3ml @19:57:31.15 CSV.dial :waste @19:57:31.88 CS.seek 0ml @19:57:39.75 Garaging FlushPuck in Collection Clamp @19:57:39.82 Elbow.reconfigure :tight @19:57:39.86 Elbow.seek CC @19:57:40.08 Elbow.reconfigure :loose @19:57:40.18 Forearm.seek CC @19:57:41.14 Hand.close @19:57:41.74 CC.seek :open @19:57:46.73 Forearm.seek :retract @19:57:47.80 Elbow.reconfigure :tight @19:57:47.85 Elbow.seek :garage @19:57:50.64 Elbow.reconfigure :loose @19:57:50.74 Forearm.reconfigure :tight @19:57:50.79 Forearm.seek :garage @19:57:51.86 Hand.open @19:57:52.55 Forearm.reconfigure :loose @19:57:52.59 Forearm.seek :retract @19:57:53.59 Starting stand-alone da WCR @19:57:53.61 da wcr sampling at most 100ml @19:57:53.64 Whole Cell Archive sampling 100ml @19:57:53.66 DAfans.email "Started stand-alone da WCR in tube 2 at 19:57:53UTC09-Jan-24\n{2 => 1}\nCTD data unavailable\nCan@19:53:36, 26% flow, 20.9C, 23% humidity, 15.0psia, 13.769V, 0.278A, 8.256Ah, 3.83W",Subject: "da wcr sampling at most 100ml" @19:57:53.67 Loading wcr puck from tube 2 into Collection Clamp @19:57:53.96 SC.reconfigure :tight @19:57:54.02 SC.seek 2 @19:57:58.03 upsync @19:58:00.73 SC.configure :limp @19:58:00.97 Elbow.reconfigure :tight @19:58:01.03 Elbow.seek 2 @19:58:02.94 Forearm.reconfigure :tight @19:58:03.00 Forearm.seek 2 + 450 counts @19:58:04.00 SE.reconfigure :pick @19:58:04.07 SE.seek :beamBreak @19:58:07.14 SE.configure :default @19:58:07.31 Elbow.seek 2 @19:58:07.50 Forearm.seek 2 + 300 counts @19:58:07.74 Forearm.reconfigure :loose @19:58:07.79 Hand.close @19:58:08.38 SE.seek :down @19:58:08.97 Forearm.seek :retract @19:58:09.94 Elbow.seek CC @19:58:11.21 SE.stop @19:58:11.50 Elbow.reconfigure :loose @19:58:11.60 Forearm.reconfigure :tight @19:58:11.65 Forearm.seek CC @19:58:12.98 CC.seek :closed @19:58:17.50 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.24 @19:58:17.52 Hand.open @19:58:18.17 Forearm.reconfigure :loose @19:58:18.22 Forearm.seek :retract @19:58:19.22 collectSample 100ml w/bubble point of 35psi @19:58:19.23 Priming 7ml @19:58:19.24 Intake.select :close @19:58:19.25 Exhaust.select :close @19:58:19.27 SS.hold @19:58:19.47 Calibrating pressure and temperature sensors @19:58:19.50 CTV.dial :air @19:58:19.58 SSV.dial :spit,avoiding: :exhaust @19:58:19.66 SS.reconfigure :fast @19:58:19.71 SS.seek 5ml @19:58:22.53 SSV.dial CBV @19:58:22.55 CBV.dial SSV @19:58:23.90 delay 3.8 seconds @19:58:27.84 Sensor limits: Intake max 108.6psia; Outlet max 103.3psia @19:58:27.85 SSV.dial :spit @19:58:29.16 SS.seek 0ml @19:58:31.96 SSV.dial CBV @19:58:33.28 CTV.dial :intake @19:58:34.06 SSV.dial CBV @19:58:34.09 CBV.dial SSV @19:58:34.29 Allowing 40s for 7.0ml of remaining 7.0ml @19:58:34.30 Intake.select :open @19:58:36.46 Intake @15.5psia, vacuum from 10.0 to 13.0psi @19:58:36.48 SS.reconfigure :inhaling 2.20ml/s @19:58:36.53 SS.seek 7ml @19:58:43.29 Stroke completed -- waiting for pressure to equalize ... @19:58:43.30 SS.reconfigure :exhale @19:58:43.35 SS.hold @19:58:46.63 Intake.select :close @19:58:46.78 SSV.dial :exhaust @19:58:47.54 Exhaust.select :open @19:58:47.65 SS.seek 0ml @19:58:51.84 Exhaust.select :close @19:58:51.95 Sampling 100.0ml @19:58:51.96 Intake.select :close @19:58:51.97 Exhaust.select :close @19:58:51.99 SS.reconfigure :default @19:58:52.03 SS.hold @19:58:52.24 CTV.dial :intake @19:58:52.48 SSV.dial CBV @19:58:52.51 CBV.dial SSV @19:58:53.29 Allowing 130s for 25.0ml of remaining 100.0ml @19:58:53.30 Intake.select :open @19:58:55.46 Intake @15.6psia, vacuum from 10.0 to 13.0psi @19:58:55.48 SS.reconfigure :inhaling 2.20ml/s @19:58:55.53 SS.seek 25ml @19:59:10.10 Stroke completed -- waiting for pressure to equalize ... @19:59:10.11 SS.reconfigure :exhale @19:59:10.16 SS.hold @19:59:14.90 Intake.select :close @19:59:15.05 SSV.dial :exhaust @19:59:15.79 Exhaust.select :open @19:59:15.90 SS.seek 0ml @19:59:25.31 Exhaust.select :close @19:59:25.42 SSV.dial CBV @19:59:25.44 CBV.dial SSV @19:59:26.20 Allowing 130s for 25.0ml of remaining 75.0ml @19:59:26.21 Intake.select :open @19:59:28.38 Intake @15.7psia, vacuum from 10.0 to 13.0psi @19:59:28.39 SS.reconfigure :inhaling 2.20ml/s @19:59:28.44 SS.seek 25ml @19:59:43.07 Stroke completed -- waiting for pressure to equalize ... @19:59:43.08 SS.reconfigure :exhale @19:59:43.13 SS.hold @19:59:48.77 Intake.select :close @19:59:48.93 SSV.dial :exhaust @19:59:49.67 Exhaust.select :open @19:59:49.78 SS.seek 0ml @19:59:58.62 Exhaust.select :close @19:59:58.73 SSV.dial CBV @19:59:58.75 CBV.dial SSV @19:59:59.52 Allowing 130s for 25.0ml of remaining 50.0ml @19:59:59.54 Intake.select :open @20:00:01.70 Intake @15.7psia, vacuum from 10.0 to 13.0psi @20:00:01.72 SS.reconfigure :inhaling 2.20ml/s @20:00:01.77 SS.seek 25ml @20:00:16.90 Stroke completed -- waiting for pressure to equalize ... @20:00:16.91 SS.reconfigure :exhale @20:00:16.96 SS.hold @20:00:23.57 Intake.select :close @20:00:23.72 SSV.dial :exhaust @20:00:24.48 Exhaust.select :open @20:00:24.59 SS.seek 0ml @20:00:33.38 Exhaust.select :close @20:00:33.49 SSV.dial CBV @20:00:33.51 CBV.dial SSV @20:00:34.32 Allowing 130s for 25.0ml of remaining 25.0ml @20:00:34.33 Intake.select :open @20:00:36.50 Intake @15.8psia, vacuum from 10.0 to 13.0psi @20:00:36.52 SS.reconfigure :inhaling 2.20ml/s @20:00:36.56 SS.seek 25ml @20:00:51.00 Vacuum too high -- waiting up to 40 seconds @20:00:51.02 SS.reconfigure :exhale @20:00:51.07 SS.hold @20:00:51.75 Intake @11.9psia, vacuum from 6.4 to 9.4psi @20:00:51.77 SS.reconfigure :inhaling 1.76ml/s @20:00:51.82 SS.seek 25ml @20:00:52.27 Stroke completed -- waiting for pressure to equalize ... @20:00:52.28 SS.reconfigure :exhale @20:00:52.33 SS.hold @20:00:59.15 Intake.select :close @20:00:59.31 SSV.dial :exhaust @20:01:00.05 Exhaust.select :open @20:01:00.17 SS.seek 0ml @20:01:09.15 Exhaust.select :close @20:01:09.26 Sampled 100.0ml @20:01:09.28 Drying puck before fixing for 5:00 @20:01:09.29 CBV.dial :waste2b @20:01:10.04 delay 1:00 @20:02:10.07 CSV.dial :air @20:02:10.92 CS.seek 10ml @20:02:35.72 Pumping wcr puck from tube 2 up to 35psi... @20:02:35.73 CBV.dial SSV @20:02:36.98 CBV.dial between CSV and waste2b @20:02:37.94 CSV.dial between air and CTV @20:02:38.27 CS.seek 7.355ml @20:02:45.09 CTV.dial CSV,avoiding: :air @20:02:45.14 CSV.dial CTV @20:02:45.92 CS.reconfigure :pressurize @20:02:45.97 CS.seek 0ml @20:03:05.30 CSV.dial :air @20:03:05.76 CS.reconfigure :fast @20:03:05.81 CS.seek 10ml @20:03:30.33 CSV.dial between air and CTV @20:03:30.66 CS.seek 3.499ml @20:03:46.34 CTV.dial CSV,avoiding: :air @20:03:46.37 CSV.dial CTV @20:03:46.73 CS.reconfigure :pressurize @20:03:46.78 CS.seek 0ml @20:03:51.26 CBV.dial :waste2b @20:03:51.60 CS.reconfigure :default @20:03:51.65 CS.seek 0ml @20:03:57.56 delay 1:00 @20:04:57.58 CTV.dial :air,avoiding: :intake @20:04:58.90 Fix #1 of 2 (with RNAlater) for 2:30 @20:04:58.92 CSV.dial CSR @20:04:59.35 Pulling 2ml of CSR.RNAlater @20:04:59.36 CSR.select :RNAlater @20:04:59.48 CS.seek 2ml @20:05:05.43 delay 5 seconds @20:05:10.45 CSR.select :air @20:05:10.57 CS.reconfigure :fast @20:05:10.62 CS.seek 10ml @20:05:30.21 Pumping wcr puck from tube 2 up to 35psi... @20:05:30.23 CBV.dial SSV @20:05:31.47 CBV.dial between CSV and waste2b @20:05:32.41 CSV.dial between air and CTV @20:05:33.44 CS.seek 7.283ml @20:05:40.46 CTV.dial CSV,avoiding: :air @20:05:40.49 CSV.dial CTV @20:05:41.84 CS.reconfigure :pressurize @20:05:41.89 CS.seek 0ml @20:06:01.02 CSV.dial :air @20:06:01.48 CS.reconfigure :fast @20:06:01.53 CS.seek 10ml @20:06:26.01 CSV.dial between air and CTV @20:06:26.34 CS.seek 4.596ml @20:06:39.43 CTV.dial CSV,avoiding: :air @20:06:39.46 CSV.dial CTV @20:06:39.84 CS.reconfigure :pressurize @20:06:39.89 CS.seek 0ml @20:06:52.14 CSV.dial :air @20:06:52.60 CS.reconfigure :fast @20:06:52.66 CS.seek 10ml @20:07:17.17 CSV.dial between air and CTV @20:07:17.50 CS.seek 4.602ml @20:07:30.65 CTV.dial CSV,avoiding: :air @20:07:30.67 CSV.dial CTV @20:07:30.98 CS.reconfigure :pressurize @20:07:31.03 CS.seek 0ml @20:07:43.27 CSV.dial :air @20:07:43.73 CS.reconfigure :fast @20:07:43.78 CS.seek 10ml @20:08:08.17 CSV.dial between air and CTV @20:08:08.50 CS.seek 4.629ml @20:08:21.49 CTV.dial CSV,avoiding: :air @20:08:21.52 CSV.dial CTV @20:08:21.82 CS.reconfigure :pressurize @20:08:21.87 CS.seek 0ml @20:08:34.24 CSV.dial :air @20:08:34.69 CS.reconfigure :default @20:08:34.74 CS.hold @20:08:34.87 ShallowSampler::Error in MAIN -- Puck.from 2, :wcr reached only 23.5 of 35psi after pumping in 40ml of gas @20:08:34.88 Waiting to Resume from Checkpoint "Pumping wcr puck from tube 2 up to 35psi..." @20:08:34.90 BadNews.email "Puck.from 2, :wcr reached only 23.5 of 35psi after pumping in 40ml of gas",Subject: "ShallowSampler::Error in MAIN" @20:08:39.16 upsync @20:10:36.49 Session Begun @20:10:46.47 -> MainThread.resume @20:10:46.50
Resuming Thread (suspended since 20:05:30) # @20:10:46.55 Pumping wcr puck from tube 2 up to 35psi... @20:10:46.60 CBV.dial SSV @20:10:48.03 CBV.dial between CSV and waste2b @20:10:49.00 CSV.dial between air and CTV @20:10:49.34 CS.reconfigure :fast @20:10:49.41 CS.seek 4.806ml @20:11:02.02 CTV.dial CSV,avoiding: :air @20:11:02.05
CSV.dial CTV @20:11:02.40 CS.reconfigure :pressurize @20:11:02.47 CS.seek 0ml @20:11:15.33 CSV.dial :air @20:11:15.80 CS.reconfigure :fast @20:11:15.88 CS.seek 10ml @20:11:40.45 CSV.dial between air and CTV @20:11:40.78 CS.seek 4.873ml @20:11:53.21 CTV.dial CSV,avoiding: :air @20:11:53.24
CSV.dial CTV @20:11:53.57 CS.reconfigure :pressurize @20:11:53.64 CS.seek 0ml @20:12:05.79 delay 2:30 @20:14:35.81 CBV.dial :waste2b @20:14:36.16 CS.reconfigure :default @20:14:36.22 CS.seek 0ml @20:14:37.98 delay 1:00 @20:15:38.01 CTV.dial :air,avoiding: :intake @20:15:39.36 Fix #2 of 2 (with RNAlater) for 2:30 @20:15:39.37 CSV.dial CSR @20:15:39.84 Pulling 2ml of CSR.RNAlater @20:15:39.85 CSR.select :RNAlater @20:15:40.00 CS.seek 2ml @20:15:45.98 delay 5 seconds @20:15:51.00 CSR.select :air @20:15:51.14 CS.reconfigure :fast @20:15:51.20 CS.seek 10ml @20:16:10.86 Pumping wcr puck from tube 2 up to 35psi... @20:16:10.88 CBV.dial SSV @20:16:12.14 CBV.dial between CSV and waste2b @20:16:13.12 CSV.dial between air and CTV @20:16:13.74 CS.seek 7.237ml @20:16:20.89 CTV.dial CSV,avoiding: :air @20:16:21.38
CSV.dial CTV @20:16:22.74 CS.reconfigure :pressurize @20:16:22.81 CS.seek 0ml @20:16:37.91 delay 2:30 @20:19:07.93 CBV.dial :waste2b @20:19:08.28 CS.reconfigure :default @20:19:08.35 CS.seek 0ml @20:19:12.96 delay 1:00 @20:20:12.98 CTV.dial :air,avoiding: :intake @20:20:14.33 Storing wcr puck in Collection Clamp to tube 1 @20:20:14.52 SC.reconfigure :tight @20:20:14.59 SC.seek 1 @20:20:18.22 SC.configure :limp @20:20:18.45 Elbow.reconfigure :tight @20:20:18.51 Elbow.seek 1 @20:20:20.29 Forearm.reconfigure :tight @20:20:20.36 Forearm.seek 1 + 450 counts @20:20:21.43 SE.reconfigure :pick @20:20:21.51 SE.seek :beamBreak @20:20:24.37 SE.configure :default @20:20:24.44 SE.seek :place @20:20:25.00 Forearm.reconfigure :loose @20:20:25.07 Forearm.seek :retract @20:20:26.06 Elbow.seek CC @20:20:27.69 Elbow.reconfigure :loose @20:20:27.81 Forearm.seek CC @20:20:28.81 Hand.close @20:20:29.48 CC.seek :open @20:20:34.26 Forearm.seek :retract @20:20:35.25 Elbow.reconfigure :tight @20:20:35.32 Elbow.seek 1 @20:20:37.13 Elbow.reconfigure :limp @20:20:37.60 Forearm.seek 1 @20:20:38.66 Elbow.hold @20:20:38.74 Elbow.reconfigure :tight @20:20:38.81 Hand.open @20:20:39.59 Forearm.force -20 @20:20:39.65 Forearm.seek 1 + 320 counts @20:20:39.95 Forearm.reconfigure :tight @20:20:40.02 Forearm.seek 1 + 450 counts @20:20:40.30 SE.seek :down @20:20:41.44
Forearm.reconfigure :loose @20:20:41.50 Forearm.seek :retract @20:20:42.99 SE.stop @20:20:43.05
Starting finalPurge @20:20:43.07 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @20:20:43.29 Elbow.seek FlushPuck::Garage @20:20:45.19 Elbow.reconfigure :loose @20:20:45.32 Forearm.seek :garage @20:20:46.33 Hand.close @20:20:47.08 Forearm.seek :retract @20:20:48.08 Elbow.reconfigure :tight @20:20:48.15 Elbow.seek CC @20:20:51.16 Elbow.reconfigure :loose @20:20:51.28 Forearm.reconfigure :tight @20:20:51.35 Forearm.seek CC @20:20:52.61 CC.seek :closed @20:20:57.63 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.25 @20:20:57.77 Hand.open @20:20:58.40 Forearm.reconfigure :loose @20:20:58.47 Forearm.seek :retract @20:20:59.45 Flushing the Collection Valve::Manifold @20:20:59.60 CSV.dial CSR @20:21:00.33 Pulling 5ml of CSR.flush @20:21:00.34 CSR.select :flush @20:21:00.49 CS.seek 5ml @20:21:14.10 delay 5 seconds @20:21:19.12 CSR.select :air @20:21:19.31 CS.reconfigure :fast @20:21:19.37 CS.seek 7.5ml @20:21:26.13 CSV.dial :waste @20:21:27.42 CS.reconfigure :default @20:21:27.48 CS.seek 0ml @20:21:47.48 Flushing lower manifold's waste2b port @20:21:47.62 CSV.dial :flush @20:21:48.07 CS.reconfigure :fast @20:21:48.14 CS.seek 5ml @20:22:01.10 delay 5 seconds @20:22:06.12 CSV.dial :air @20:22:06.65 CS.seek 7ml @20:22:12.16 CSV.dial CTV @20:22:12.19
CTV.dial CSV @20:22:13.55 CBV.dial :waste2b @20:22:13.64 CS.seek 0ml @20:22:30.19 Disinfecting external Intake with kill @20:22:30.33 CSV.dial CSR @20:22:30.79 Pulling 8ml of CSR.kill @20:22:30.80 CSR.select :kill @20:22:30.95 CS.seek 8ml @20:22:50.75 delay 5 seconds @20:22:55.77 CSR.select :air @20:22:55.90 CS.seek 10ml @20:23:01.45 CSV.dial CBV @20:23:01.47
CBV.dial CSV @20:23:02.66 CTV.dial :intake @20:23:03.44 Intake.select :open @20:23:03.57 CS.seek 2ml @20:23:22.38 Intake.select :close @20:23:22.50 CTV.dial :air @20:23:23.26 CS.reconfigure :default @20:23:23.32 CS.seek 0ml @20:23:29.32 CSV.dial CSR @20:23:30.32 Pulling 5ml of CSR.flush @20:23:30.33 CSR.select :flush @20:23:30.47 CS.seek 5ml @20:23:44.07 delay 5 seconds @20:23:49.08 CSR.select :air @20:23:49.22 CS.seek 10ml @20:24:02.94 CSV.dial CBV @20:24:04.40 CS.seek 0ml @20:24:30.82 Garaging FlushPuck in Collection Clamp @20:24:30.91 Elbow.reconfigure :tight @20:24:30.98 Elbow.seek CC @20:24:31.41 Elbow.reconfigure :loose @20:24:31.53 Forearm.seek CC @20:24:32.51 Hand.close @20:24:33.09 CC.seek :open @20:24:37.74 Forearm.seek :retract @20:24:38.78 Elbow.reconfigure :tight @20:24:38.85 Elbow.seek :garage @20:24:41.60 Elbow.reconfigure :loose @20:24:41.72 Forearm.reconfigure :tight @20:24:41.78 Forearm.seek :garage @20:24:42.74 Hand.open @20:24:43.40 Forearm.reconfigure :loose @20:24:43.46 Forearm.seek :retract @20:24:44.43 Completed [initialPurge; standAloneWCR([nil,100], $da)] begun 19:53:36 @20:24:44.50 Asleep after completing standAloneWCR_010224 assays @20:24:44.52 GoodNews.email "{1 => 1,\n 2 => 0}\nCTD data unavailable\nCan@20:22:50, 26% flow, 21.5C, 23% humidity, 15.0psia, 13.808V, 0.429A, 8.471Ah, 5.93W\nSampled 100.0ml",Subject: "Asleep after completing standAloneWCR_010224 assays" @20:24:48.73 upsync @20:24:48.83 Session Ended + ESPwhich standAloneWCR_010224.rb + fn=standAloneWCR_010224.rb + IFS=: + set /home/esp/esp2/mission . + fullPath=/home/esp/esp2/mission/standAloneWCR_010224.rb + [ -f /home/esp/esp2/mission/standAloneWCR_010224.rb ] + realpath /home/esp/esp2/mission/standAloneWCR_010224.rb + return 0 + scriptPath=/home/esp/esp2local/mission/standAloneWCR_010224.rb + exec ruby /home/esp/esp2/lib/esp.rb /home/esp/esp2local/mission/standAloneWCR_010224.rb @21:05:44.14UTC09-Jan-24
ESP2 v3.42 I2C Gateway v3.20 for core bus @21:05:46.84 Configuration for short puck jake 1/11/21 by brent@mbari.org /home/esp/esp2/type/shallow/jake/configure.rb @21:05:50.52 Polling Can every 10:00 @21:05:50.53 Cleanly exited at 21:05:31UTC09-Jan-24 @21:05:50.58 Loading /home/esp/esp2local/mission/standAloneWCR_010224.rb mission @21:05:50.64 Preparing to sleep until + 00:00:05 @21:05:50.66 GoodNews.email "Starting standAloneWCR_010224 w/pucks in tube 2\n{2 => 1}\nCTD data unavailable\nCan@21:05:50, 25% flow, 21.2C, 23% humidity, 15.0psia, 13.803V, 0.321A, 8.713Ah, 4.42W",Subject: "standAloneWCR_010224 begun" @21:05:50.70
Asleep til + 00:00:05 @21:05:50.75 Continuing at 21:05 @21:05:50.98 Gate.power :core,:OFF @21:05:50.99 Gate.power :raw,:OFF @21:05:51.00 Shutdown @21:05:53.86 GoodNews.email "Then, in tube 2: initialPurge; standAloneWCR([nil,100], $da)\n/home/esp/esp2local/mission/standAloneWCR_010224.rb:10\n{2 => 1}\nCTD data unavailable\nCan@21:05:50, 26% flow, 21.2C, 22% humidity, 15.0psia, 13.808V, 0.321A, 8.713Ah, 4.43W",Subject: "Asleep until 21:05" @21:05:55.21 upsync @21:05:55.62
Powering core bus @21:05:58.19 upsync @21:06:01.06
Gate.power :raw,:ON @21:06:02.04 CS.configure :default @21:06:02.09 PS.configure :default @21:06:02.14 SS.configure :default @21:06:02.19 CC.configure :default @21:06:02.23 PC.configure :default @21:06:02.30 Forearm.configure :loose @21:06:02.35 Elbow.configure :loose @21:06:02.42 SC.configure :loose @21:06:02.46 SE.configure :default @21:06:02.51 CH.configure :default @21:06:02.55 PH.configure :default @21:06:02.58 All dwarf controllers running firmware version 3.85 @21:06:02.69 Valve.reset @21:06:02.94 Intake.select :close @21:06:02.99 Exhaust.select :close @21:06:03.01 CTV.dial :air @21:06:03.11 PSV.dial :air @21:06:03.21
Forearm.home.seek :retract @21:06:03.28 CSV.dial :waste1 @21:06:03.32 PS.home.seek 0ml @21:06:03.78 SSV.dial :spit,avoiding: :exhaust @21:06:03.94 Hand.close @21:06:03.98
Elbow.reconfigure :loose @21:06:04.05 Elbow.jog 1500 @21:06:04.55 Elbow.reconfigure :loose @21:06:04.62 Elbow.home.seek :clear @21:06:04.69 CS.reconfigure :fast @21:06:04.72 SS.home.seek 0ml @21:06:04.81 CS.home.seek 0ml @21:06:07.85
SE.home.seek :down @21:06:08.46 SC.reconfigure :tight @21:06:08.51 SC.home.seek :initial @21:06:11.64 PS.stop @21:06:33.88
SC.configure :limp @21:06:33.93 Forearm.seek :retract @21:06:33.99 Elbow.seek :hammer @21:06:35.10 Forearm.reconfigure :tight @21:06:35.15 Forearm.seek retracted @21:06:35.41 PC.home.seek :open @21:06:35.43
CC.home.seek :open @21:06:38.87 Notice.email "At 21:06:38 began in tube 2: initialPurge; standAloneWCR([nil,100], $da)\n/home/esp/esp2local/mission/standAloneWCR_010224.rb:10\n{2 => 1}\nCTD data unavailable\nCan@21:06:38, 25% flow, 21.2C, 23% humidity, 15.0psia, 13.773V, 0.290A, 8.718Ah, 4.00W",Subject: "Started standAloneWCR_010224 phase initialPurge; standAloneWCR([nil,100], $da)" @21:06:38.90
Phase [initialPurge; standAloneWCR([nil,100], $da)] @21:06:38.93 Starting initialPurge @21:06:38.94 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @21:06:39.01 Hand.open @21:06:39.80 Elbow.reconfigure :tight @21:06:39.85 Elbow.seek FlushPuck::Garage @21:06:41.73 Elbow.reconfigure :loose @21:06:41.85 Forearm.reconfigure :loose @21:06:41.90 Forearm.seek :garage @21:06:43.00 Hand.close @21:06:43.31 upsync @21:06:43.68
Forearm.seek :retract @21:06:44.68 Elbow.reconfigure :tight @21:06:44.72 Elbow.seek CC @21:06:47.60 Elbow.reconfigure :loose @21:06:47.70 Forearm.reconfigure :tight @21:06:47.75 Forearm.seek CC @21:06:48.99 CC.seek :closed @21:06:53.57 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.32 @21:06:53.64 Hand.open @21:06:54.37 Forearm.reconfigure :loose @21:06:54.41 Forearm.seek :retract @21:06:55.40 Flushing the Collection Valve::Manifold @21:06:55.52 CSV.dial CSR @21:06:56.79 Pulling 5ml of CSR.flush CSR.select :flush @21:06:56.91 CS.reconfigure :default @21:06:56.96 CS.seek 5ml @21:07:10.75 delay 5 seconds @21:07:16.14 CSR.select :air @21:07:16.37 CS.reconfigure :fast @21:07:16.42 CS.seek 7.501ml @21:07:23.16 CSV.dial :waste @21:07:24.45 CS.reconfigure :default @21:07:24.49 CS.seek 0ml @21:07:44.50 Purging external Intake @21:07:44.63 CSV.dial :flush @21:07:45.07 CS.reconfigure :fast @21:07:45.12 CS.seek 10ml @21:08:09.63 delay 5 seconds @21:08:14.65 CSV.dial CBV @21:08:14.69
CBV.dial CSV @21:08:15.42 CTV.dial :intake @21:08:16.16 Intake.select :open @21:08:16.27 CS.seek 0ml @21:08:39.94 Intake.select :close @21:08:40.04 CTV.dial :air @21:08:40.80 Flushing upper manifold @21:08:40.92 CSV.dial :flush @21:08:41.67 CS.seek 5ml @21:08:54.56 delay 5 seconds @21:08:59.58 CSV.dial :air @21:09:00.08 CS.seek 7ml @21:09:05.66 CSV.dial CBV @21:09:05.69
CBV.dial CSV @21:09:06.72 CTV.dial :air @21:09:06.74 CS.seek 0ml @21:09:23.25 delay 7 seconds @21:09:30.28 CS.seek 3ml @21:09:38.34 CSV.dial :waste @21:09:38.80 CS.seek 0ml @21:09:46.64 CSV.dial :flush @21:09:47.09 CS.seek 3ml @21:09:55.13 delay 5 seconds @21:10:00.14 CSV.dial :air @21:10:00.64 CS.seek 5ml @21:10:06.25 CSV.dial :air @21:10:06.33 CS.seek 0ml @21:10:18.41 delay 7 seconds @21:10:25.44 CS.seek 3ml @21:10:33.66 CSV.dial :waste @21:10:34.40 CS.seek 0ml @21:10:42.16 Garaging FlushPuck in Collection Clamp @21:10:42.23 Elbow.reconfigure :tight @21:10:42.27 Elbow.seek CC @21:10:42.59 Elbow.reconfigure :loose @21:10:42.69 Forearm.seek CC @21:10:43.72 Hand.close @21:10:44.30 CC.seek :open @21:10:49.28 Forearm.seek :retract @21:10:50.35 Elbow.reconfigure :tight @21:10:50.40 Elbow.seek :garage @21:10:53.33 Elbow.reconfigure :loose @21:10:53.42 Forearm.reconfigure :tight @21:10:53.47 Forearm.seek :garage @21:10:54.49 Hand.open @21:10:55.11 Forearm.reconfigure :loose @21:10:55.16 Forearm.seek :retract @21:10:56.14 Starting stand-alone da WCR @21:10:56.15 da wcr sampling at most 100ml @21:10:56.18 Whole Cell Archive sampling 100ml @21:10:56.20 DAfans.email "Started stand-alone da WCR in tube 2 at 21:10:56UTC09-Jan-24\n{2 => 1}\nCTD data unavailable\nCan@21:06:38, 25% flow, 21.2C, 23% humidity, 15.0psia, 13.773V, 0.290A, 8.718Ah, 4.00W",Subject: "da wcr sampling at most 100ml" @21:10:56.24 Loading wcr puck from tube 2 into Collection Clamp @21:10:56.51 SC.reconfigure :tight @21:10:56.56 SC.seek 2 @21:11:00.66 upsync @21:11:03.33 SC.configure :limp @21:11:03.56 Elbow.reconfigure :tight @21:11:03.61 Elbow.seek 2 @21:11:05.55 Forearm.reconfigure :tight @21:11:05.60 Forearm.seek 2 + 450 counts @21:11:06.63 SE.reconfigure :pick @21:11:06.69 SE.seek :beamBreak @21:11:09.79 SE.configure :default @21:11:09.95 Elbow.seek 2 @21:11:10.14 Forearm.seek 2 + 300 counts @21:11:10.38 Forearm.reconfigure :loose @21:11:10.43 Hand.close @21:11:11.07 SE.seek :down @21:11:11.66 Forearm.seek :retract @21:11:12.71 Elbow.seek CC @21:11:13.87 SE.stop @21:11:14.36 Elbow.reconfigure :loose @21:11:14.46 Forearm.reconfigure :tight @21:11:14.51 Forearm.seek CC @21:11:15.74 CC.seek :closed @21:11:20.26 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.25 @21:11:20.27 Hand.open @21:11:20.99 Forearm.reconfigure :loose @21:11:21.04 Forearm.seek :retract @21:11:22.01 collectSample 100ml w/bubble point of 35psi @21:11:22.02 Priming 7ml @21:11:22.03 Intake.select :close @21:11:22.04 Exhaust.select :close @21:11:22.06 SS.hold @21:11:22.25 Calibrating pressure and temperature sensors @21:11:22.29 CTV.dial :air @21:11:22.30 SSV.dial :spit,avoiding: :exhaust @21:11:22.38 SS.reconfigure :fast @21:11:22.43 SS.seek 5ml @21:11:25.33 SSV.dial CBV @21:11:25.35 CBV.dial SSV @21:11:26.72 delay 3.8 seconds @21:11:30.64 Sensor limits: Intake max 107.7psia; Outlet max 103.0psia @21:11:30.65 SSV.dial :spit @21:11:31.97 SS.seek 0ml @21:11:34.84 SSV.dial CBV @21:11:36.15 CTV.dial :intake @21:11:36.92 SSV.dial CBV @21:11:36.97 CBV.dial SSV @21:11:37.14 Allowing 40s for 7.0ml of remaining 7.0ml @21:11:37.15 Intake.select :open @21:11:39.31 Intake @15.5psia, vacuum from 10.0 to 13.0psi @21:11:39.33 SS.reconfigure :inhaling 2.20ml/s @21:11:39.38 SS.seek 7ml @21:11:46.65 Stroke completed -- waiting for pressure to equalize ... @21:11:46.66 SS.reconfigure :exhale @21:11:46.71 SS.hold @21:11:49.95 Intake.select :close @21:11:50.10 SSV.dial :exhaust @21:11:50.87 Exhaust.select :open @21:11:50.98 SS.seek 0ml @21:11:55.10 Exhaust.select :close @21:11:55.22 Sampling 100.0ml @21:11:55.23 Intake.select :close @21:11:55.24 Exhaust.select :close @21:11:55.25 SS.reconfigure :default @21:11:55.30 SS.hold @21:11:55.50 CTV.dial :intake @21:11:55.58 SSV.dial CBV @21:11:55.60 CBV.dial SSV @21:11:56.36 Allowing 130s for 25.0ml of remaining 100.0ml @21:11:56.37 Intake.select :open @21:11:58.53 Intake @15.8psia, vacuum from 10.0 to 13.0psi @21:11:58.55 SS.reconfigure :inhaling 2.20ml/s @21:11:58.60 SS.seek 25ml @21:12:13.62 Stroke completed -- waiting for pressure to equalize ... @21:12:13.63 SS.reconfigure :exhale @21:12:13.68 SS.hold @21:12:18.51 Intake.select :close @21:12:18.68 SSV.dial :exhaust @21:12:19.45 Exhaust.select :open @21:12:19.56 SS.seek 0ml @21:12:28.40 Exhaust.select :close @21:12:28.96 SSV.dial CBV @21:12:28.98 CBV.dial SSV @21:12:29.75 Allowing 130s for 25.0ml of remaining 75.0ml @21:12:29.76 Intake.select :open @21:12:31.93 Intake @15.7psia, vacuum from 10.0 to 13.0psi @21:12:31.94 SS.reconfigure :inhaling 2.20ml/s @21:12:31.99 SS.seek 25ml @21:12:47.43 Stroke completed -- waiting for pressure to equalize ... @21:12:47.44 SS.reconfigure :exhale @21:12:47.49 SS.hold @21:12:53.88 Intake.select :close @21:12:54.03 SSV.dial :exhaust @21:12:54.79 Exhaust.select :open @21:12:54.90 SS.seek 0ml @21:13:03.79 Exhaust.select :close @21:13:03.90 SSV.dial CBV @21:13:03.93 CBV.dial SSV @21:13:04.70 Allowing 130s for 25.0ml of remaining 50.0ml @21:13:04.71 Intake.select :open @21:13:06.87 Intake @15.7psia, vacuum from 10.0 to 13.0psi @21:13:06.89 SS.reconfigure :inhaling 2.20ml/s @21:13:06.94 SS.seek 25ml @21:13:22.04 Stroke completed -- waiting for pressure to equalize ... @21:13:22.05 SS.reconfigure :exhale @21:13:22.10 SS.hold @21:13:29.64 Intake.select :close @21:13:29.80 SSV.dial :exhaust @21:13:30.54 Exhaust.select :open @21:13:30.65 SS.seek 0ml @21:13:39.59 Exhaust.select :close @21:13:39.70 SSV.dial CBV @21:13:39.72 CBV.dial SSV @21:13:40.48 Allowing 130s for 25.0ml of remaining 25.0ml @21:13:40.49 Intake.select :open @21:13:42.65 Intake @15.8psia, vacuum from 10.0 to 13.0psi @21:13:42.67 SS.reconfigure :inhaling 2.20ml/s @21:13:42.72 SS.seek 25ml @21:13:54.75 Vacuum too high -- waiting up to 40 seconds @21:13:54.77 SS.reconfigure :exhale @21:13:54.82 SS.hold @21:13:55.48 Intake @12.3psia, vacuum from 6.8 to 9.8psi @21:13:55.49 SS.reconfigure :inhaling 1.76ml/s @21:13:55.54 SS.seek 25ml @21:14:00.19 Stroke completed -- waiting for pressure to equalize ... @21:14:00.20 SS.reconfigure :exhale @21:14:00.25 SS.hold @21:14:05.86 Intake.select :close @21:14:06.02 SSV.dial :exhaust @21:14:07.21 Exhaust.select :open @21:14:07.32 SS.seek 0ml @21:14:16.10 Exhaust.select :close @21:14:16.22 Sampled 100.0ml @21:14:16.24 Drying puck before fixing for 5:00 @21:14:16.25 CBV.dial :waste2b @21:14:17.00 delay 1:00 @21:15:17.03 CSV.dial :air @21:15:17.77 CS.seek 10ml @21:15:42.31 Pumping wcr puck from tube 2 up to 35psi... @21:15:42.33 CBV.dial SSV @21:15:43.59 CBV.dial between CSV and waste2b @21:15:44.56 CSV.dial between air and CTV @21:15:44.99 CS.seek 7.293ml @21:15:52.00 CTV.dial CSV,avoiding: :air @21:15:52.03 CSV.dial CTV @21:15:52.81 CS.reconfigure :pressurize @21:15:52.86 CS.seek 0ml @21:16:09.92 CBV.dial :waste2b @21:16:10.25 CS.reconfigure :default @21:16:10.30 CS.seek 0ml @21:16:13.28 delay 1:00 @21:17:13.30 CTV.dial :air,avoiding: :intake @21:17:14.62 Fix #1 of 2 (with RNAlater) for 2:30 @21:17:14.64 CSV.dial CSR @21:17:15.07 Pulling 2ml of CSR.RNAlater @21:17:15.08 CSR.select :RNAlater @21:17:15.20 CS.seek 2ml @21:17:21.12 delay 5 seconds @21:17:26.14 CSR.select :air @21:17:26.26 CS.reconfigure :fast @21:17:26.31 CS.seek 10ml @21:17:45.96 Pumping wcr puck from tube 2 up to 35psi... @21:17:45.98 CBV.dial SSV @21:17:47.23 CBV.dial between CSV and waste2b @21:17:48.19 CSV.dial between air and CTV @21:17:48.80 CS.seek 7.26ml @21:17:55.87 CTV.dial CSV,avoiding: :air @21:17:55.90 CSV.dial CTV @21:17:57.25 CS.reconfigure :pressurize @21:17:57.30 CS.seek 0ml @21:18:11.67 delay 2:30 @21:20:41.69 CBV.dial :waste2b @21:20:42.02 CS.reconfigure :default @21:20:42.07 CS.seek 0ml @21:20:47.59 delay 1:00 @21:21:47.62 CTV.dial :air,avoiding: :intake @21:21:48.92 Fix #2 of 2 (with RNAlater) for 2:30 @21:21:48.93 CSV.dial CSR @21:21:49.47 Pulling 2ml of CSR.RNAlater @21:21:49.48 CSR.select :RNAlater @21:21:49.99 CS.seek 2ml @21:21:55.96 delay 5 seconds @21:22:00.98 CSR.select :air @21:22:01.10 CS.reconfigure :fast @21:22:01.15 CS.seek 10ml @21:22:20.89 Pumping wcr puck from tube 2 up to 35psi... @21:22:20.91 CBV.dial SSV @21:22:22.17 CBV.dial between CSV and waste2b @21:22:23.12 CSV.dial between air and CTV @21:22:23.71 CS.seek 7.243ml @21:22:30.77 CTV.dial CSV,avoiding: :air @21:22:30.80 CSV.dial CTV @21:22:32.15 CS.reconfigure :pressurize @21:22:32.20 CS.seek 0ml @21:22:46.91 delay 2:30 @21:25:16.94 CBV.dial :waste2b @21:25:17.27 CS.reconfigure :default @21:25:17.32 CS.seek 0ml @21:25:22.55 delay 1:00 @21:26:22.58 CTV.dial :air,avoiding: :intake @21:26:23.93 Storing wcr puck in Collection Clamp to tube 1 @21:26:24.11 SC.reconfigure :tight @21:26:24.16 SC.seek 1 @21:26:27.80 SC.configure :limp @21:26:28.01 Elbow.reconfigure :tight @21:26:28.06 Elbow.seek 1 @21:26:29.84 Forearm.reconfigure :tight @21:26:29.89 Forearm.seek 1 + 450 counts @21:26:30.94 SE.reconfigure :pick @21:26:31.00 SE.seek :beamBreak @21:26:33.83 SE.configure :default @21:26:33.88 SE.seek :place @21:26:34.45 Forearm.reconfigure :loose @21:26:34.50 Forearm.seek :retract @21:26:35.43 Elbow.seek CC @21:26:37.06 Elbow.reconfigure :loose @21:26:37.59 Forearm.seek CC @21:26:38.57 Hand.close @21:26:39.18 CC.seek :open @21:26:43.93 Forearm.seek :retract @21:26:44.93 Elbow.reconfigure :tight @21:26:44.98 Elbow.seek 1 @21:26:46.77 Elbow.reconfigure :limp @21:26:46.83 Forearm.seek 1 @21:26:47.83 Elbow.hold @21:26:47.90 Elbow.reconfigure :tight @21:26:47.95 Hand.open @21:26:48.65 Forearm.force -20 @21:26:48.70 Forearm.seek 1 + 302 counts @21:26:48.99 Forearm.reconfigure :tight @21:26:49.04 Forearm.seek 1 + 450 counts @21:26:49.29 SE.seek :down @21:26:50.42 Forearm.reconfigure :loose @21:26:50.47 Forearm.seek :retract @21:26:51.96 SE.stop @21:26:52.00
Starting finalPurge @21:26:52.02 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @21:26:52.20 Elbow.seek FlushPuck::Garage @21:26:53.97 Elbow.reconfigure :loose @21:26:54.06 Forearm.seek :garage @21:26:55.02 Hand.close @21:26:55.78 Forearm.seek :retract @21:26:56.77 Elbow.reconfigure :tight @21:26:56.82 Elbow.seek CC @21:26:59.75 Elbow.reconfigure :loose @21:27:00.30 Forearm.reconfigure :tight @21:27:00.35 Forearm.seek CC @21:27:01.63 CC.seek :closed @21:27:06.20 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.37 @21:27:06.27 Hand.open @21:27:06.97 Forearm.reconfigure :loose @21:27:07.01 Forearm.seek :retract @21:27:08.02 Flushing the Collection Valve::Manifold @21:27:08.14 CSV.dial CSR @21:27:08.68 Pulling 5ml of CSR.flush @21:27:08.69 CSR.select :flush @21:27:08.81 CS.seek 5ml @21:27:22.54 delay 5 seconds @21:27:27.56 CSR.select :air @21:27:27.73 CS.reconfigure :fast @21:27:27.77 CS.seek 7.501ml @21:27:34.51 CSV.dial :waste @21:27:35.78 CS.reconfigure :default @21:27:35.83 CS.seek 0ml @21:27:56.01 Flushing lower manifold's waste2b port @21:27:56.14 CSV.dial :flush @21:27:56.60 CS.reconfigure :fast @21:27:56.65 CS.seek 5ml @21:28:09.48 delay 5 seconds @21:28:14.50 CSV.dial :air @21:28:14.99 CS.seek 7ml @21:28:20.61 CSV.dial CTV @21:28:20.64
CTV.dial CSV @21:28:21.98 CBV.dial :waste2b @21:28:22.00 CS.seek 0ml @21:28:38.76 Disinfecting external Intake with kill @21:28:38.88 CSV.dial CSR @21:28:39.31 Pulling 8ml of CSR.kill @21:28:39.32 CSR.select :kill @21:28:39.43 CS.seek 8ml @21:28:59.32 delay 5 seconds @21:29:04.34 CSR.select :air @21:29:04.46 CS.seek 10ml @21:29:09.95 CSV.dial CBV @21:29:09.98
CBV.dial CSV @21:29:11.03 CTV.dial :intake @21:29:11.79 Intake.select :open @21:29:11.91 CS.seek 2ml @21:29:30.85 Intake.select :close @21:29:30.96 CTV.dial :air @21:29:31.70 CS.reconfigure :default @21:29:31.75 CS.seek 0ml @21:29:37.92 CSV.dial CSR @21:29:38.93 Pulling 5ml of CSR.flush @21:29:38.94 CSR.select :flush @21:29:39.06 CS.seek 5ml @21:29:52.64 delay 5 seconds @21:29:57.66 CSR.select :air @21:29:57.78 CS.seek 10ml @21:30:11.42 CSV.dial CBV @21:30:12.42 CS.seek 0ml @21:30:38.79 Garaging FlushPuck in Collection Clamp @21:30:38.86 Elbow.reconfigure :tight @21:30:38.91 Elbow.seek CC @21:30:39.17 Elbow.reconfigure :loose @21:30:39.27 Forearm.seek CC @21:30:40.33 Hand.close @21:30:40.91 CC.seek :open @21:30:45.56 Forearm.seek :retract @21:30:46.54 Elbow.reconfigure :tight @21:30:46.58 Elbow.seek :garage @21:30:49.45 Elbow.reconfigure :loose @21:30:49.55 Forearm.reconfigure :tight @21:30:49.59 Forearm.seek :garage @21:30:50.55 Hand.open @21:30:51.22 Forearm.reconfigure :loose @21:30:51.26 Forearm.seek :retract @21:30:52.24 Completed [initialPurge; standAloneWCR([nil,100], $da)] begun 21:06:38 @21:30:52.29 Asleep after completing standAloneWCR_010224 assays @21:30:52.30 GoodNews.email "{1 => 1,\n 2 => 0}\nCTD data unavailable\nCan@21:25:53, 26% flow, 21.2C, 23% humidity, 15.0psia, 13.715V, 0.435A, 8.861Ah, 5.97W\nSampled 100.0ml",Subject: "Asleep after completing standAloneWCR_010224 assays" @21:30:56.48 upsync + ESPwhich DA_WCR_011024.rb + fn=DA_WCR_011024.rb + IFS=: + set /home/esp/esp2/mission . + fullPath=/home/esp/esp2/mission/DA_WCR_011024.rb + [ -f /home/esp/esp2/mission/DA_WCR_011024.rb ] + realpath /home/esp/esp2/mission/DA_WCR_011024.rb + return 0 + scriptPath=/home/esp/esp2local/mission/DA_WCR_011024.rb + exec ruby /home/esp/esp2/lib/esp.rb /home/esp/esp2local/mission/DA_WCR_011024.rb @19:25:14.72UTC10-Jan-24
ESP2 v3.42 I2C Gateway v3.20 for core bus @19:25:17.41 Configuration for short puck jake 1/11/21 by brent@mbari.org /home/esp/esp2/type/shallow/jake/configure.rb @19:25:21.11 Polling Can every 10:00 @19:25:21.13 Cleanly exited at 19:22:45UTC10-Jan-24 @19:25:21.17 Loading /home/esp/esp2local/mission/DA_WCR_011024.rb mission @19:25:21.19 RuntimeError in MAIN -- Mission's startTube should be 3, not 2 @19:25:21.21 Waiting to Resume from Checkpoint "Loading /home/esp/esp2local/mission/DA_WCR_011024.rb mission" @19:25:21.23 BadNews.email "Mission's startTube should be 3, not 2",Subject: "RuntimeError in MAIN" @19:25:26.01 upsync + ESPwhich DA_WCR_011024.rb + fn=DA_WCR_011024.rb + IFS=: + set /home/esp/esp2/mission . + fullPath=/home/esp/esp2/mission/DA_WCR_011024.rb + [ -f /home/esp/esp2/mission/DA_WCR_011024.rb ] + realpath /home/esp/esp2/mission/DA_WCR_011024.rb + return 0 + scriptPath=/home/esp/esp2local/mission/DA_WCR_011024.rb + exec ruby /home/esp/esp2/lib/esp.rb /home/esp/esp2local/mission/DA_WCR_011024.rb Log::Locked in trapHandler -- Another process is already writing to /var/log/esp/real.log + ESPwhich DA_WCR_011024.rb + fn=DA_WCR_011024.rb + IFS=: + set /home/esp/esp2/mission . + fullPath=/home/esp/esp2/mission/DA_WCR_011024.rb + [ -f /home/esp/esp2/mission/DA_WCR_011024.rb ] + realpath /home/esp/esp2/mission/DA_WCR_011024.rb + return 0 + scriptPath=/home/esp/esp2local/mission/DA_WCR_011024.rb + exec ruby /home/esp/esp2/lib/esp.rb /home/esp/esp2local/mission/DA_WCR_011024.rb @19:33:26.99UTC10-Jan-24
ESP2 v3.42 I2C Gateway v3.20 for core bus @19:33:29.56 Configuration for short puck jake 1/11/21 by brent@mbari.org /home/esp/esp2/type/shallow/jake/configure.rb @19:33:33.53 Polling Can every 10:00 @19:33:33.56 Cleanly exited at 19:33:10UTC10-Jan-24 @19:33:33.60 Loading /home/esp/esp2local/mission/DA_WCR_011024.rb mission @19:33:33.66 GoodNews.email "Starting DA_WCR_011024 w/pucks in tube 2\n{1 => 0,\n 2 => 4,\n 3 => 0,\n 4 => 0,\n 5 => 0,\n 6 => 0,\n 7 => 0}\nCTD data unavailable\nCan@19:33:33, 25% flow, 20.2C, 28% humidity, 14.5psia, 13.744V, 0.272A, 0.200Ah, 3.74W",Subject: "DA_WCR_011024 begun" @19:33:33.70
Preparing to sleep until + 00:00:05 @19:33:33.73 Asleep til + 00:00:05 @19:33:33.74 Continuing at 19:33 @19:33:34.03 Gate.power :core,:OFF @19:33:34.04 Gate.power :raw,:OFF @19:33:34.05 Shutdown @19:33:37.56 GoodNews.email "Then, in tube 2: initialPurge; da [50, 50] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_011024.rb:14\n{1 => 0,\n 2 => 4,\n 3 => 0,\n 4 => 0,\n 5 => 0,\n 6 => 0,\n 7 => 0}\nCTD data unavailable\nCan@19:33:33, 25% flow, 20.2C, 28% humidity, 14.5psia, 13.744V, 0.272A, 0.200Ah, 3.74W",Subject: "Asleep until 19:33" @19:33:38.65
Powering core bus @19:33:39.24 upsync @19:33:42.65 upsync @19:33:44.17
Gate.power :raw,:ON @19:33:45.21 CS.configure :default @19:33:45.26 PS.configure :default @19:33:45.31 SS.configure :default @19:33:45.36 CC.configure :default @19:33:45.41 PC.configure :default @19:33:45.48 Forearm.configure :loose @19:33:45.53 Elbow.configure :loose @19:33:45.60 SC.configure :loose @19:33:45.65 SE.configure :default @19:33:45.69 CH.configure :default @19:33:45.73 PH.configure :default @19:33:45.77 All dwarf controllers running firmware version 3.85 @19:33:45.88 Valve.reset @19:33:46.09 Intake.select :close @19:33:46.17 Exhaust.select :close @19:33:46.20 CTV.dial :air @19:33:46.66 PSV.dial :air @19:33:46.69 CSV.dial :waste1 @19:33:46.88 SSV.dial :spit,avoiding: :exhaust @19:33:46.90
Forearm.home.seek :retract @19:33:46.93 PS.home.seek 0ml @19:33:47.18 CS.reconfigure :fast @19:33:47.21 SS.home.seek 0ml @19:33:47.31 CS.home.seek 0ml @19:33:47.71 Hand.close @19:33:47.74
Elbow.reconfigure :loose @19:33:47.84 Elbow.jog 1500 @19:33:48.37 Elbow.reconfigure :loose @19:33:48.41 Elbow.home.seek :clear @19:33:52.89 SE.home.seek :down @19:33:53.47 SC.reconfigure :tight @19:33:53.52 SC.home.seek :initial @19:33:55.21 PS.stop @19:34:21.83
SC.configure :limp @19:34:21.88 Forearm.seek :retract @19:34:21.95 Elbow.seek :hammer @19:34:22.96 Forearm.reconfigure :tight @19:34:23.01 Forearm.seek retracted @19:34:23.27 PC.home.seek :open @19:34:23.28
CC.home.seek :open @19:34:26.75 Phase [initialPurge; da [50, 50] #da and wsr are run simultanelously] @19:34:26.77 Notice.email "At 19:34:26 began in tube 2: initialPurge; da [50, 50] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_011024.rb:14\n{1 => 0,\n 2 => 4,\n 3 => 0,\n 4 => 0,\n 5 => 0,\n 6 => 0,\n 7 => 0}\nCTD data unavailable\nCan@19:34:26, 25% flow, 20.1C, 28% humidity, 14.5psia, 13.778V, 0.290A, 0.205Ah, 4.00W",Subject: "Started DA_WCR_011024 phase initialPurge; da [50, 50] #da and wsr are run simultanelously" @19:34:26.79
Starting initialPurge @19:34:26.81 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @19:34:26.88 Hand.open @19:34:27.60 Elbow.reconfigure :tight @19:34:27.65 Elbow.seek FlushPuck::Garage @19:34:29.56 Elbow.reconfigure :loose @19:34:29.66 Forearm.reconfigure :loose @19:34:29.71 Forearm.seek :garage @19:34:30.70 Hand.close @19:34:31.47 Forearm.seek :retract @19:34:32.24 upsync @19:34:32.45
Elbow.reconfigure :tight @19:34:32.50 Elbow.seek CC @19:34:35.44 Elbow.reconfigure :loose @19:34:35.54 Forearm.reconfigure :tight @19:34:35.59 Forearm.seek CC @19:34:36.94 CC.seek :closed @19:34:41.48 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.41 @19:34:41.55 Hand.open @19:34:42.28 Forearm.reconfigure :loose @19:34:42.33 Forearm.seek :retract @19:34:43.32 Flushing the Collection Valve::Manifold @19:34:43.45 CSV.dial CSR @19:34:44.72 Pulling 5ml of CSR.flush @19:34:44.73 CSR.select :flush @19:34:44.85 CS.reconfigure :default @19:34:44.90 CS.seek 5ml @19:34:58.79 delay 5 seconds @19:35:03.80 CSR.select :air @19:35:04.38 CS.reconfigure :fast @19:35:04.43 CS.seek 7.5ml @19:35:11.31 CSV.dial :waste @19:35:12.60 CS.reconfigure :default @19:35:12.65 CS.seek 0ml @19:35:32.88 Purging external Intake @19:35:33.00 CSV.dial :flush @19:35:33.57 CS.reconfigure :fast @19:35:33.62 CS.seek 10ml @19:35:58.41 delay 5 seconds @19:36:03.43 CSV.dial CBV @19:36:03.46
CBV.dial CSV @19:36:04.19 CTV.dial :intake @19:36:04.94 Intake.select :open @19:36:05.05 CS.seek 0ml @19:36:28.78 Intake.select :close @19:36:28.89 CTV.dial :air @19:36:29.66 Flushing upper manifold @19:36:29.78 CSV.dial :flush @19:36:30.52 CS.seek 5ml @19:36:43.44 delay 5 seconds @19:36:48.46 CSV.dial :air @19:36:48.95 CS.seek 7.001ml @19:36:54.53 CSV.dial CBV @19:36:54.55
CBV.dial CSV @19:36:55.59 CTV.dial :air @19:36:55.68 CS.seek 0ml @19:37:12.38 delay 7 seconds @19:37:19.40 CS.seek 3ml @19:37:27.56 CSV.dial :waste @19:37:28.02 CS.seek 0ml @19:37:36.02 CSV.dial :flush @19:37:36.46 CS.seek 3ml @19:37:44.63 delay 5 seconds @19:37:49.65 CSV.dial :air @19:37:50.26 CS.seek 5ml @19:37:56.03 CSV.dial :air @19:37:56.12 CS.seek 0ml @19:38:08.34 delay 7 seconds @19:38:15.37 CS.seek 3ml @19:38:23.72 CSV.dial :waste @19:38:24.44 CS.seek 0ml @19:38:32.13 Garaging FlushPuck in Collection Clamp @19:38:32.20 Elbow.reconfigure :tight @19:38:32.24 Elbow.seek CC @19:38:32.54 Elbow.reconfigure :loose @19:38:32.64 Forearm.seek CC @19:38:33.69 Hand.close @19:38:34.34 CC.seek :open @19:38:39.36 Forearm.seek :retract @19:38:40.40 Elbow.reconfigure :tight @19:38:40.44 Elbow.seek :garage @19:38:43.32 Elbow.reconfigure :loose @19:38:43.43 Forearm.reconfigure :tight @19:38:43.48 Forearm.seek :garage @19:38:44.50 Hand.open @19:38:45.14 Forearm.reconfigure :loose @19:38:45.19 Forearm.seek :retract @19:38:46.19 da sampling at most 50ml, wcr at most 50ml @19:38:46.23 Loading sh1 puck from tube 2 into Collection Clamp @19:38:46.25 DAfans.email "Started da in tube 2 at 19:38:46UTC10-Jan-24\n{1 => 0,\n 2 => 4,\n 3 => 0,\n 4 => 0,\n 5 => 0,\n 6 => 0,\n 7 => 0}\nCTD data unavailable\nCan@19:34:26, 25% flow, 20.1C, 28% humidity, 14.5psia, 13.778V, 0.290A, 0.205Ah, 4.00W",Subject: "da sampling at most 50ml, wcr at most 50ml" @19:38:46.53
SC.reconfigure :tight @19:38:46.59 SC.seek 2 @19:38:50.33 upsync @19:38:53.28
SC.configure :limp @19:38:53.52 Elbow.reconfigure :tight @19:38:53.56 Elbow.seek 2 @19:38:55.48 Forearm.reconfigure :tight @19:38:55.53 Forearm.seek 2 + 450 counts @19:38:56.52 SE.reconfigure :pick @19:38:56.58 SE.seek :beamBreak @19:38:59.48 SE.configure :default @19:38:59.64 Elbow.seek 2 @19:38:59.83 Forearm.seek 2 + 300 counts @19:39:00.02 Forearm.reconfigure :loose @19:39:00.06 Hand.close @19:39:00.72 SE.seek :down @19:39:00.93
Forearm.seek :retract @19:39:01.88 Elbow.seek CC @19:39:03.30 SE.stop @19:39:03.51
Elbow.reconfigure :loose @19:39:03.62 Forearm.reconfigure :tight @19:39:03.66 Forearm.seek CC @19:39:04.87 CC.seek :closed @19:39:09.39 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.28 @19:39:09.40 Hand.open @19:39:10.02 Forearm.reconfigure :loose @19:39:10.06 Forearm.seek :retract @19:39:11.05 collectSample 50ml w/bubble point of 24psi @19:39:11.06 Priming 7ml @19:39:11.07 Intake.select :close @19:39:11.08 Exhaust.select :close @19:39:11.09 SS.hold @19:39:11.30 Calibrating pressure and temperature sensors @19:39:11.33 CTV.dial :air @19:39:11.41 SSV.dial :spit,avoiding: :exhaust @19:39:11.43 SS.reconfigure :fast @19:39:11.48 SS.seek 5ml @19:39:14.38 SSV.dial CBV @19:39:14.39
CBV.dial SSV @19:39:15.75 delay 3.8 seconds @19:39:19.67 Sensor limits: Intake max 96.1psia; Outlet max 91.3psia @19:39:19.68 SSV.dial :spit @19:39:21.00 SS.seek 0ml @19:39:23.81 SSV.dial CBV @19:39:25.10 CTV.dial :intake @19:39:25.89 SSV.dial CBV @19:39:25.91
CBV.dial SSV @19:39:26.11 Allowing 40s for 7.0ml of remaining 7.0ml @19:39:26.12 Intake.select :open @19:39:28.29 Intake @15.1psia, vacuum from 9.6 to 12.6psi @19:39:28.30 SS.reconfigure :inhaling 2.20ml/s @19:39:28.35 SS.seek 7ml @19:39:35.05 Stroke completed -- waiting for pressure to equalize ... @19:39:35.06 SS.reconfigure :exhale @19:39:35.11 SS.hold @19:39:37.55 Intake.select :close @19:39:37.70 SSV.dial :exhaust @19:39:38.45 Exhaust.select :open @19:39:38.56 SS.seek 0ml @19:39:42.85 Exhaust.select :close @19:39:42.96 Sampling 50.0ml @19:39:42.97 Intake.select :close @19:39:42.99 Exhaust.select :close @19:39:43.00 SS.reconfigure :default @19:39:43.04 SS.hold @19:39:43.25 CTV.dial :intake @19:39:43.43 SSV.dial CBV @19:39:43.47
CBV.dial SSV @19:39:44.21 Allowing 130s for 25.0ml of remaining 50.0ml @19:39:44.22 Intake.select :open @19:39:46.39 Intake @15.2psia, vacuum from 9.7 to 12.7psi @19:39:46.41 SS.reconfigure :inhaling 2.20ml/s @19:39:46.46 SS.seek 25ml @19:40:01.02 Stroke completed -- waiting for pressure to equalize ... @19:40:01.03 SS.reconfigure :exhale @19:40:01.08 SS.hold @19:40:05.23 Intake.select :close @19:40:05.38 SSV.dial :exhaust @19:40:06.12 Exhaust.select :open @19:40:06.24 SS.seek 0ml @19:40:15.05 Exhaust.select :close @19:40:15.16 SSV.dial CBV @19:40:15.19
CBV.dial SSV @19:40:15.95 Allowing 130s for 25.0ml of remaining 25.0ml @19:40:15.96 Intake.select :open @19:40:18.12 Intake @15.3psia, vacuum from 9.8 to 12.8psi @19:40:18.14 SS.reconfigure :inhaling 2.20ml/s @19:40:18.18 SS.seek 25ml @19:40:33.77 Stroke completed -- waiting for pressure to equalize ... @19:40:33.78 SS.reconfigure :exhale @19:40:33.83 SS.hold @19:40:38.31 Intake.select :close @19:40:38.47 SSV.dial :exhaust @19:40:39.22 Exhaust.select :open @19:40:39.33 SS.seek 0ml @19:40:48.24 Exhaust.select :close @19:40:48.35 Sampled 50.0ml @19:40:48.37 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @19:40:48.38 Intake.select :close @19:40:48.44 CSV.dial :waste @19:40:48.60 SSV.dial CBV,avoiding: :exhaust @19:40:48.62 CS.seek 0ml @19:40:48.65
CBV.dial SSV @19:40:48.81 CSV.dial :air @19:40:49.55 CS.seek 10ml @19:40:50.54
CTV.dial :air @19:40:51.31 delay 15 seconds @19:41:06.33 SSV.dial :spit,avoiding: :exhaust @19:41:07.66 CTV.dial CSV,avoiding: :intake @19:41:07.69
SS.seek 0ml @19:41:07.82 SSV.dial CBV,avoiding: :exhaust @19:41:14.09 SSV.dial between CBV and plug @19:41:14.59 CBV.dial between SSV and waste2 @19:41:15.00 CTV.dial CSV,avoiding: :air @19:41:15.02
CSV.dial CTV @19:41:15.51 CS.reconfigure :pressurize @19:41:15.56 CS.seek 0ml @19:41:34.32 BubblePoint: Intake @24.2psia, Outlet @0.5psia @19:41:34.33 CBV.dial SSV @19:41:34.66 SS.reconfigure :default @19:41:34.71 SS.seek 10ml @19:41:40.38 delay 15 seconds @19:41:55.51 Evacuated: Intake @23.7psia, Outlet @23.8psia @19:41:55.52 SSV.dial :spit,avoiding: :exhaust @19:41:56.54 SS.reconfigure :exhale @19:41:56.59 SS.seek 0ml @19:42:01.64 SS.reconfigure :fast @19:42:01.69 SS.seek 5ml @19:42:04.48 SSV.dial CBV,avoiding: :exhaust @19:42:04.51
CBV.dial SSV @19:42:05.87 CTV.dial :air,avoiding: :intake @19:42:07.20 CSV.dial CTV @19:42:07.24
SS.seek 10ml @19:42:08.80 CSV.dial :air @19:42:09.31 CS.reconfigure :fast @19:42:09.37 CS.seek 0ml @19:42:09.81
SSV.dial :spit,avoiding: :exhaust @19:42:11.12 SS.reconfigure :exhale @19:42:11.17 SS.seek 0ml @19:42:16.56 SS.reconfigure :fast @19:42:16.61 SS.seek 5ml @19:42:16.74 CS.stop @19:42:19.51 SSV.dial CBV,avoiding: :exhaust @19:42:19.53
CBV.dial SSV @19:42:21.89 SSV.dial :spit,avoiding: :exhaust @19:42:23.18 SS.reconfigure :default @19:42:23.23 SS.seek 0ml @19:42:26.60 SS.stop @19:42:26.64 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @19:42:26.65 Intake.select :close @19:42:26.72 CSV.dial :waste @19:42:26.86 SSV.dial CBV,avoiding: :exhaust @19:42:26.87
CBV.dial SSV @19:42:27.49 CS.seek 0ml @19:42:27.56 CSV.dial :air @19:42:28.23
CTV.dial :air @19:42:28.31 delay 15 seconds @19:42:28.41 CS.seek 10ml @19:42:43.33
SSV.dial :spit,avoiding: :exhaust @19:42:44.64 CTV.dial CSV,avoiding: :intake @19:42:44.67
SS.reconfigure :exhale @19:42:44.76 SS.seek 0ml @19:42:45.19 SSV.dial CBV,avoiding: :exhaust @19:42:52.78 SSV.dial between CBV and plug @19:42:53.26 CBV.dial between SSV and waste2 @19:42:53.65 CSV.dial between air and CTV @19:42:53.97 CS.seek 8.299ml @19:42:58.66 CTV.dial CSV,avoiding: :air @19:42:58.68
CSV.dial CTV @19:42:59.05 CS.reconfigure :pressurize @19:42:59.10 CS.seek 0ml @19:43:17.47 BubblePoint: Intake @24.1psia, Outlet @0.6psia @19:43:17.48 CBV.dial SSV @19:43:17.81 SS.reconfigure :default @19:43:17.86 SS.seek 10ml @19:43:23.51 delay 15 seconds @19:43:38.63 Evacuated: Intake @23.6psia, Outlet @17.9psia @19:43:38.65 SSV.dial :spit,avoiding: :exhaust @19:43:39.68 SS.reconfigure :exhale @19:43:39.73 SS.seek 0ml @19:43:44.80 SS.reconfigure :fast @19:43:44.84 SS.seek 5ml @19:43:47.68 SSV.dial CBV,avoiding: :exhaust @19:43:47.70
CBV.dial SSV @19:43:49.05 CTV.dial :air,avoiding: :intake @19:43:50.37 CSV.dial CTV @19:43:50.41
SS.seek 10ml @19:43:51.63 CSV.dial :air @19:43:52.06 CS.reconfigure :fast @19:43:52.11 CS.seek 0ml @19:43:52.96
SSV.dial :spit,avoiding: :exhaust @19:43:54.28 SS.reconfigure :exhale @19:43:54.32 SS.seek 0ml @19:43:55.99 CS.stop @19:43:59.67
SS.reconfigure :fast @19:43:59.72 SS.seek 5ml @19:44:02.59 SSV.dial CBV,avoiding: :exhaust @19:44:02.61
CBV.dial SSV @19:44:04.95 SSV.dial :spit,avoiding: :exhaust @19:44:06.24 SS.reconfigure :default @19:44:06.29 SS.seek 0ml @19:44:09.66 SS.stop @19:44:09.70 Lysing: Adding 2.0ml AqMeOH @19:44:09.71 CBV.dial between CSV and waste1,avoiding: :DBV @19:44:11.18 CTV.dial CSV @19:44:12.48 CSV.dial CSR @19:44:13.38 Pulling 1ml of CSR.AqMeOH @19:44:13.39 CSR.select :AqMeOH @19:44:13.50 CS.reconfigure :default @19:44:13.55 CS.seek 1ml @19:44:17.12 delay 5 seconds @19:44:22.14 CSR.select :air @19:44:22.30 CS.seek 6ml @19:44:35.93 delay 2 seconds @19:44:37.95 CSV.dial :waste2 @19:44:38.41 CS.reconfigure :fast @19:44:38.45 CS.seek 0ml @19:44:52.99 CSV.dial CSR @19:44:53.45 Pulling 2ml of CSR.AqMeOH CSR.select :AqMeOH @19:44:53.57 CS.reconfigure :default @19:44:53.61 CS.seek 2ml @19:44:59.59 delay 5 seconds @19:45:04.60 CSR.select :air @19:45:04.77 CS.seek 5.001ml @19:45:13.34 delay 2 seconds @19:45:15.35 CSV.dial CTV @19:45:15.80 CS.seek 1ml @19:45:27.07 Before lysing Collection Puck Heater at 20C/24.6psia @19:45:27.09 Heating Puck.from 2, :sh1 in Collection Clamp to 70C for 10:00 @19:45:27.11 CH.seek 70C,8:00 @19:47:20.85 CH.hold 10:00,70C @19:47:21.05 Begun lysing Collection Puck Heater at 68C/30.0psia @19:57:21.06 Ended lysing Collection Puck Heater at 70C/28.7psia @19:57:21.08 CS.seek 0ml @19:57:24.45 CTV.dial between spare and air,avoiding: :intake @19:57:25.48 CSV.dial :air @19:57:25.92 CS.seek 5ml @19:57:39.63 CSV.dial CTV @19:57:40.09 delay 2 seconds @19:57:42.11 CSV.dial :air @19:57:42.56 delay 2 seconds @19:57:44.57 CSV.dial CTV @19:57:45.03 delay 2 seconds @19:57:47.05 CSV.dial :air @19:57:47.50 delay 2 seconds @19:57:49.52 CS.seek 0ml @19:58:03.13 Puck @57.2psia after final lysis pressurization @19:58:03.14 Recovering Lysate into Collection syringe @19:58:03.15 CSV.dial CBV @19:58:04.60 CBV.dial CSV @19:58:05.00 CS.seek 5ml @19:58:18.67 delay 5 seconds @19:58:23.69 CTV.dial :air @19:58:24.20 CS.seek 7ml @19:58:30.07 delay 5 seconds @19:58:35.09 CSV.dial :air2 @19:58:35.53 CS.seek 10ml @19:58:44.07 CSV.dial between air and CTV,avoiding: CBV @19:58:45.22 Lysing Complete @19:58:45.28 Storing sh1 puck in Collection Clamp to tube 1 @19:58:45.46 SC.reconfigure :tight @19:58:45.50 SC.seek 1 @19:58:49.10 SC.configure :limp @19:58:49.23 SE.reconfigure :place @19:58:49.33
Elbow.reconfigure :tight @19:58:49.38 Elbow.seek CC @19:58:49.40 SE.seek :beamBreak @19:58:49.65
Elbow.reconfigure :loose @19:58:49.75 Forearm.seek CC @19:58:50.80 Hand.close @19:58:51.44 CC.seek :open @19:58:52.28 SE.configure :default @19:58:52.33 SE.seek :place @19:58:56.18
Forearm.seek :retract @19:58:57.15 Elbow.reconfigure :tight @19:58:57.19 Elbow.seek 1 @19:58:58.96 Elbow.reconfigure :limp @19:58:59.01 Forearm.seek 1 @19:59:00.00 Elbow.hold @19:59:00.07 Elbow.reconfigure :tight @19:59:00.11 Hand.open @19:59:00.81 Forearm.force -20 @19:59:00.85 Forearm.seek 1 + 294 counts @19:59:01.25 Forearm.reconfigure :tight @19:59:01.71 Forearm.seek 1 + 450 counts @19:59:02.01 SE.seek :down @19:59:03.14
Forearm.reconfigure :loose @19:59:03.19 Forearm.seek :retract @19:59:04.71 SE.stop @19:59:04.75
Tranferring DA @19:59:04.77 Beginning samplePassC @19:59:04.78 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @19:59:04.96 Elbow.seek FlushPuck::Garage @19:59:06.73 Elbow.reconfigure :loose @19:59:06.83 Forearm.seek :garage @19:59:07.84 Hand.close @19:59:08.56 Forearm.seek :retract @19:59:09.55 Elbow.reconfigure :tight @19:59:09.60 Elbow.seek CC @19:59:12.56 Elbow.reconfigure :loose @19:59:12.67 Forearm.reconfigure :tight @19:59:12.71 Forearm.seek CC @19:59:14.06 CC.seek :closed @19:59:18.58 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.37 @19:59:18.65 Hand.open @19:59:19.37 Forearm.reconfigure :loose @19:59:19.42 Forearm.seek :retract @19:59:20.41 PSV.dial PSR @19:59:21.80 Pulling 1.5ml of PSR.flush @19:59:21.81 PSR.select :flush @19:59:21.93 PS.seek 1.5ml @19:59:38.11 delay 5 seconds @19:59:43.14 PSV.dial :waste1 @19:59:43.64 PS.seek 0ml @19:59:59.80 PSV.dial PSR @20:00:00.27 PSR.select :air @20:00:00.39 PS.seek 2.5ml @20:00:26.78 PRV.dial CBV,avoiding: :ab2 @20:00:26.80
CBV.dial PRV @20:00:28.55 PSV.dial :mixloop @20:00:29.60 CTV.dial :air,avoiding: :intake @20:00:29.68 PTV.dial :PRVmixing @20:00:31.01 PS.seek 0ml @20:00:57.33 PSV.dial :air @20:00:57.81 PS.seek 2.5ml @20:01:24.14 PSV.dial :mixloop @20:01:24.63 PS.seek 0ml @20:01:51.05 PSV.dial :air @20:01:51.52 PS.seek 2.5ml @20:02:17.92 PSV.dial :mixloop @20:02:18.40 PS.seek 0ml @20:02:44.71 Garaging FlushPuck in Collection Clamp @20:02:44.78 Elbow.reconfigure :tight @20:02:44.83 Elbow.seek CC @20:02:45.09 Elbow.reconfigure :loose @20:02:45.19 Forearm.seek CC @20:02:46.22 Hand.close @20:02:46.82 CC.seek :open @20:02:51.47 Forearm.seek :retract @20:02:52.44 Elbow.reconfigure :tight @20:02:52.48 Elbow.seek :garage @20:02:55.37 Elbow.reconfigure :loose @20:02:55.47 Forearm.reconfigure :tight @20:02:55.52 Forearm.seek :garage @20:02:56.57 Hand.open @20:02:57.25 Forearm.reconfigure :loose @20:02:57.29 Forearm.seek :retract @20:02:58.31 PSV.dial :mixloop @20:02:58.39 PTV.dial :PRVmixing @20:02:58.47 PRV.dial CBV @20:02:58.50
CBV.dial PRV @20:02:59.08 CTV.dial :air,avoiding: :intake @20:02:59.17 Loading lyfil puck from tube 2 into Collection Clamp @20:02:59.40 SC.reconfigure :tight @20:02:59.45 SC.seek 2 @20:03:03.07 SC.configure :limp @20:03:03.29 Elbow.reconfigure :tight @20:03:03.34 Elbow.seek 2 @20:03:05.21 Forearm.reconfigure :tight @20:03:05.26 Forearm.seek 2 + 450 counts @20:03:06.50 SE.reconfigure :pick @20:03:06.55 SE.seek :beamBreak @20:03:09.53 SE.configure :default @20:03:09.69 Elbow.seek 2 @20:03:09.87 Forearm.seek 2 + 300 counts @20:03:10.12 Forearm.reconfigure :loose @20:03:10.17 Hand.close @20:03:10.83 SE.seek :down @20:03:11.02
Forearm.seek :retract @20:03:12.03 Elbow.seek CC @20:03:13.49 SE.stop @20:03:13.62
Elbow.reconfigure :loose @20:03:13.72 Forearm.reconfigure :tight @20:03:13.77 Forearm.seek CC @20:03:14.98 CC.seek :closed @20:03:19.51 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.17 @20:03:19.52 Hand.open @20:03:20.25 Forearm.reconfigure :loose @20:03:20.30 Forearm.seek :retract @20:03:21.25 CTV.dial CSV,avoiding: :intake @20:03:22.61 CSV.dial CTV @20:03:22.93 PS.seek 2.5ml @20:03:49.33 CS.seek 4.501ml @20:04:04.16 delay 8 seconds @20:04:12.18 PSV.dial :air @20:04:12.66 delay 2 seconds @20:04:14.67 PSV.dial :waste2 @20:04:15.54 PS.seek 0ml @20:04:41.92 CBV.dial between PRV and waste1 @20:04:42.25 CSV.dial :air @20:04:42.71 CS.seek 10ml @20:04:57.60 CSV.dial CTV @20:04:58.06 CS.seek 0ml @20:05:24.66 CBV.dial :waste1 @20:05:24.97 delay 5 seconds @20:05:30.00 CTV.dial :air,avoiding: :intake @20:05:31.41 Storing lyfil puck in Collection Clamp to tube 1 @20:05:31.59 SC.reconfigure :tight @20:05:31.63 SC.seek 1 @20:05:35.28 SC.configure :limp @20:05:35.50 Elbow.reconfigure :tight @20:05:35.55 Elbow.seek 1 @20:05:37.32 Forearm.reconfigure :tight @20:05:37.36 Forearm.seek 1 + 450 counts @20:05:38.39 SE.reconfigure :pick @20:05:38.82 SE.seek :beamBreak @20:05:41.92 SE.configure :default @20:05:42.08 Elbow.reconfigure :limp @20:05:42.13 Forearm.reconfigure :loose @20:05:42.18 Forearm.seek 1 @20:05:42.40 Elbow.hold @20:05:42.47 Elbow.reconfigure :tight @20:05:42.57 SE.seek 0.17 Pucks below Top Plate @20:05:42.98 Forearm.force -20 @20:05:43.02 Forearm.seek 1 + 316 counts @20:05:43.30 Forearm.reconfigure :tight @20:05:43.35 Forearm.seek 1 + 450 counts @20:05:43.55 SE.seek 1.17 Pucks below Top Plate @20:05:44.06 Forearm.reconfigure :loose @20:05:44.11 Forearm.seek :retract @20:05:45.08 Elbow.seek CC @20:05:46.74 Elbow.reconfigure :loose @20:05:46.84 Forearm.seek CC @20:05:47.82 Hand.close @20:05:48.44 CC.seek :open @20:05:53.18 Forearm.seek :retract @20:05:54.17 Elbow.reconfigure :tight @20:05:54.22 Elbow.seek 1 @20:05:55.96 Elbow.reconfigure :limp @20:05:56.01 Forearm.seek 1 @20:05:56.99 Elbow.hold @20:05:57.06 Elbow.reconfigure :tight @20:05:57.11 Hand.open @20:05:58.25 Forearm.force -20 @20:05:58.29 Forearm.seek 1 + 312 counts @20:05:58.70 Forearm.reconfigure :tight @20:05:58.74 Forearm.seek 1 + 450 counts @20:05:59.03 SE.seek :down @20:06:00.16
Forearm.reconfigure :loose @20:06:00.21 Forearm.seek :retract @20:06:01.64 SE.stop @20:06:01.69
PSV.dial :air @20:06:02.44 PS.seek 2.5ml @20:06:28.79 delay 1 second @20:06:29.81 PSV.dial :mixloop @20:06:30.26 delay 1 second @20:06:31.28 PSV.dial :air @20:06:31.75 delay 1 second @20:06:32.76 PSV.dial :mixloop @20:06:33.23 delay 1 second @20:06:34.25 PSV.dial :air @20:06:34.72 delay 1 second @20:06:35.74 PS.seek 0ml @20:07:02.11 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @20:07:02.29 Elbow.seek FlushPuck::Garage @20:07:04.14 Elbow.reconfigure :loose @20:07:04.25 Forearm.seek :garage @20:07:05.20 Hand.close @20:07:05.93 Forearm.seek :retract @20:07:06.94 Elbow.reconfigure :tight @20:07:06.98 Elbow.seek PC @20:07:08.24 Elbow.reconfigure :loose @20:07:08.34 Forearm.reconfigure :tight @20:07:08.39 Forearm.seek PC @20:07:10.03 PC.seek :closed @20:07:14.70 Processing Clamp onSpring/offSpring for 16/26 samples, on/off torque 1.55 @20:07:14.77 Hand.open @20:07:15.49 Forearm.reconfigure :loose @20:07:15.54 Forearm.seek :retract @20:07:16.51 PRV.dial :air @20:07:16.98 PSV.dial :mixloop @20:07:17.51 PS.seek 0.3ml @20:07:21.39 PSV.dial PSR @20:07:22.45 Pulling 0.4ml of PSR.air @20:07:22.46 PSR.select :air @20:07:22.48 PS.seek 0.7ml @20:07:27.42 delay 3 seconds @20:07:30.43 PSR.select :air @20:07:30.46 PS.seek 2.5ml @20:07:49.73 delay 3 seconds @20:07:52.75 PSV.dial :mixloop @20:07:53.81 PTV.dial :mixing @20:07:54.57 PBV.dial :waste2 @20:07:55.53 PS.seek 0ml @20:08:21.92 Garaging FlushPuck in Processing Clamp @20:08:21.99 Elbow.reconfigure :tight @20:08:22.04 Elbow.seek PC @20:08:22.16 Elbow.reconfigure :loose @20:08:22.26 Forearm.seek PC @20:08:23.24 Hand.close @20:08:23.81 PC.seek :open @20:08:28.54 Forearm.seek :retract @20:08:29.56 Elbow.reconfigure :tight @20:08:29.61 Elbow.seek :garage @20:08:30.99 Elbow.reconfigure :loose @20:08:31.53 Forearm.reconfigure :tight @20:08:31.58 Forearm.seek :garage @20:08:32.55 Hand.open @20:08:33.24 Forearm.reconfigure :loose @20:08:33.29 Forearm.seek :retract @20:08:34.29 Loading da puck from tube 2 into Processing Clamp @20:08:34.53 SC.reconfigure :tight @20:08:34.57 SC.seek 2 @20:08:38.11 SC.configure :limp @20:08:38.33 Elbow.reconfigure :tight @20:08:38.37 Elbow.seek 2 @20:08:40.22 Forearm.reconfigure :tight @20:08:40.27 Forearm.seek 2 + 450 counts @20:08:41.37 SE.reconfigure :pick @20:08:41.42 SE.seek :beamBreak @20:08:44.49 SE.configure :default @20:08:44.65 Elbow.seek 2 @20:08:44.84 Forearm.seek 2 + 300 counts @20:08:45.04 Forearm.reconfigure :loose @20:08:45.09 Hand.close @20:08:45.70 SE.seek :down @20:08:45.90
Forearm.seek :retract @20:08:46.86 Elbow.seek PC @20:08:48.39 SE.stop @20:08:49.38
Elbow.reconfigure :loose @20:08:49.48 Forearm.reconfigure :tight @20:08:49.53 Forearm.seek PC @20:08:50.82 PC.seek :closed @20:08:55.98 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.61 @20:08:55.99 Hand.open @20:08:56.62 Forearm.reconfigure :loose @20:08:56.66 Forearm.seek :retract @20:08:57.67 Heating Puck.from 2, :da in Processing Clamp to 28C for 2:00:00 @20:08:57.69 PH.seek 28C,5:00 @20:09:29.40 PH.hold 2:00:00,28C @20:09:29.46 delay 1:30 @20:10:59.49 PSR.select :air @20:10:59.51 PSV.dial PSR @20:11:00.57 Pulling 0.5ml of PSR.wash @20:11:00.58 PSR.select :wash @20:11:00.70 PS.seek 0.5ml @20:11:06.66 delay 5 seconds @20:11:11.68 PSR.select :air @20:11:11.80 PS.seek 2ml @20:11:28.04 PTV.dial :puck @20:11:29.24 PSV.dial PTV @20:11:30.01 PBV.dial :waste1 @20:11:31.42 PS.seek 1.5ml @20:11:37.35 delay 3 seconds @20:11:40.37 PBV.dial between PSV and waste1 @20:11:40.73 PS.seek 0ml @20:11:56.82 delay 5:00 @20:16:56.84 recoverPSRreagent08 @20:16:56.85 PTV.dial :puck @20:16:57.03 PBV.dial :waste1 @20:16:57.41 delay 30 seconds @20:17:27.43 PBV.dial between PSV and waste1 @20:17:27.80 PSR.select :air @20:17:27.81 PSV.dial PSR @20:17:28.58 PS.reconfigure :fast @20:17:28.63 PS.seek 2.5ml @20:17:52.78 PSV.dial PTV @20:17:53.55 delay 5 seconds @20:17:58.57 PSV.dial PSR @20:17:59.35 delay 5 seconds @20:18:04.37 PSV.dial PTV @20:18:05.13 delay 5 seconds @20:18:10.15 PSV.dial :air @20:18:11.02 PS.seek 0ml @20:18:34.41 PTV.dial :PRVmixing,via: PRV @20:18:35.75 PSV.dial :mixloop @20:18:36.20 PRV.dial :ab1 @20:18:36.94 PS.reconfigure :slow @20:18:36.99 PS.seek 0.15ml @20:18:40.62 delay 2 seconds @20:18:42.64 CBV.dial PRV @20:18:42.67
PRV.dial CBV @20:18:43.23 PS.seek 0.34ml @20:18:47.59 delay 2 seconds @20:18:49.61 CBV.dial between PRV and PSV @20:18:49.93 PRV.dial :air @20:18:50.38 PS.seek 0.9ml @20:19:02.34 PRV.dial :waste2 @20:19:03.10 PS.seek 0.5ml @20:19:11.78 addPMLReagent08 @20:19:11.79 PSV.dial :air @20:19:12.25 PS.reconfigure :default @20:19:12.30 PS.seek 2ml @20:19:28.56 PSV.dial :PML @20:19:29.03 PTV.dial :mixing @20:19:29.78 PBV.dial :waste1 @20:19:30.20 PS.seek 1.85ml @20:19:32.65 PBV.dial between PSV and waste1 @20:19:33.46 PS.seek 0ml @20:19:53.22 delay 2 seconds @20:19:55.24 Beginning samplePassC @20:19:55.25 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @20:19:55.43 Elbow.reconfigure :tight @20:19:55.48 Elbow.seek FlushPuck::Garage @20:19:56.82 Elbow.reconfigure :loose @20:19:56.92 Forearm.seek :garage @20:19:57.91 Hand.close @20:19:58.68 Forearm.seek :retract @20:19:59.66 Elbow.reconfigure :tight @20:19:59.71 Elbow.seek CC @20:20:02.56 Elbow.reconfigure :loose @20:20:02.66 Forearm.reconfigure :tight @20:20:02.71 Forearm.seek CC @20:20:03.92 CC.seek :closed @20:20:08.49 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.48 @20:20:08.56 Hand.open @20:20:09.24 Forearm.reconfigure :loose @20:20:09.29 Forearm.seek :retract @20:20:10.25 PSV.dial PSR @20:20:11.31 Pulling 1.5ml of PSR.flush @20:20:11.32 PSR.select :flush @20:20:11.44 PS.seek 1.5ml @20:20:27.56 delay 5 seconds @20:20:32.58 PSV.dial :waste1 @20:20:33.05 PS.seek 0ml @20:20:49.59 PSV.dial PSR @20:20:50.06 PSR.select :air @20:20:50.18 PS.seek 2.5ml @20:21:16.72 PRV.dial CBV,avoiding: :ab2 @20:21:16.74
CBV.dial PRV @20:21:18.37 PSV.dial :mixloop @20:21:19.42 CTV.dial :air,avoiding: :intake @20:21:19.50 PTV.dial :PRVmixing @20:21:20.27 PS.seek 0ml @20:21:46.65 PSV.dial :air @20:21:47.13 PS.seek 2.5ml @20:22:13.40 PSV.dial :mixloop @20:22:13.89 PS.seek 0ml @20:22:40.21 PSV.dial :air @20:22:40.69 PS.seek 2.5ml @20:23:07.09 PSV.dial :mixloop @20:23:07.56 PS.seek 0ml @20:23:33.96 Garaging FlushPuck in Collection Clamp @20:23:34.04 Elbow.reconfigure :tight @20:23:34.09 Elbow.seek CC @20:23:34.32 Elbow.reconfigure :loose @20:23:34.42 Forearm.seek CC @20:23:35.45 Hand.close @20:23:36.05 CC.seek :open @20:23:40.67 Forearm.seek :retract @20:23:41.64 Elbow.reconfigure :tight @20:23:41.69 Elbow.seek :garage @20:23:44.60 Elbow.reconfigure :loose @20:23:44.70 Forearm.reconfigure :tight @20:23:44.75 Forearm.seek :garage @20:23:45.78 Hand.open @20:23:46.48 Forearm.reconfigure :loose @20:23:46.53 Forearm.seek :retract @20:23:47.49 delayUntil "20:27:55.22" @20:23:47.51 Whole Cell Archive sampling 50ml @20:23:47.53 Loading wcr puck from tube 2 into Collection Clamp @20:23:48.27 SC.reconfigure :tight @20:23:48.32 SC.seek 2 @20:23:48.60 SC.configure :limp @20:23:48.82 Elbow.reconfigure :tight @20:23:48.88 Elbow.seek 2 @20:23:50.76 Forearm.reconfigure :tight @20:23:50.81 Forearm.seek 2 + 450 counts @20:23:51.86 SE.reconfigure :pick @20:23:51.92 SE.seek :beamBreak @20:23:54.95 SE.configure :default @20:23:55.12 Elbow.seek 2 @20:23:55.31 Forearm.seek 2 + 300 counts @20:23:55.57 Forearm.reconfigure :loose @20:23:55.62 Hand.close @20:23:56.31 Puck::Warning in WCR -- Loaded Last remaining Puck (from Tube 2) @20:23:56.34 SE.seek :down @20:23:56.55 Forearm.seek :retract @20:23:57.47 Elbow.seek CC @20:23:59.11 Elbow.reconfigure :loose @20:23:59.19 SE.stop @20:23:59.29 Forearm.reconfigure :tight @20:23:59.34 Forearm.seek CC @20:24:01.02 CC.seek :closed @20:24:05.62 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.26 @20:24:05.64 Hand.open @20:24:06.36 Forearm.reconfigure :loose @20:24:06.42 Forearm.seek :retract @20:24:07.39 collectSample 50ml w/bubble point of 35psi @20:24:07.40 Priming 7ml @20:24:07.41 Intake.select :close @20:24:07.43 Exhaust.select :close @20:24:07.44 SS.hold @20:24:07.65 Calibrating pressure sensors @20:24:07.67 CTV.dial :air @20:24:07.74 SSV.dial :spit,avoiding: :exhaust @20:24:07.82 SS.reconfigure :fast @20:24:07.87 SS.seek 5ml @20:24:10.60 SSV.dial CBV @20:24:10.62 CBV.dial SSV @20:24:12.00 delay 3.8 seconds @20:24:15.94 Sensor limits: Intake max 99.4psia; Outlet max 92.0psia @20:24:15.95 SSV.dial :spit @20:24:17.33 SS.seek 0ml @20:24:20.44 SSV.dial CBV @20:24:21.77 CTV.dial :intake @20:24:22.54 SSV.dial CBV @20:24:22.57 CBV.dial SSV @20:24:22.71 Allowing 40s for 7.0ml of remaining 7.0ml @20:24:22.73 Intake.select :open @20:24:24.90 Intake @15.2psia, vacuum from 9.7 to 12.7psi @20:24:24.91 SS.reconfigure :inhaling 2.20ml/s @20:24:24.96 SS.seek 7ml @20:24:30.92 Stroke completed -- waiting for pressure to equalize ... @20:24:30.93 SS.reconfigure :exhale @20:24:30.98 SS.hold @20:24:36.88 Intake.select :close @20:24:37.04 SSV.dial :exhaust @20:24:37.83 Exhaust.select :open @20:24:37.95 SS.seek 0ml @20:24:42.03 Exhaust.select :close @20:24:42.14 Sampling 50.0ml @20:24:42.15 Intake.select :close @20:24:42.17 Exhaust.select :close @20:24:42.18 SS.reconfigure :default @20:24:42.22 SS.hold @20:24:42.44 CTV.dial :intake @20:24:42.68 SSV.dial CBV @20:24:42.72 CBV.dial SSV @20:24:43.47 Allowing 130s for 25.0ml of remaining 50.0ml @20:24:43.49 Intake.select :open @20:24:45.65 Intake @15.7psia, vacuum from 10.0 to 13.0psi @20:24:45.67 SS.reconfigure :inhaling 2.20ml/s @20:24:45.72 SS.seek 25ml @20:25:00.67 Stroke completed -- waiting for pressure to equalize ... @20:25:00.68 SS.reconfigure :exhale @20:25:00.73 SS.hold @20:25:06.05 Intake.select :close @20:25:06.21 SSV.dial :exhaust @20:25:06.97 Exhaust.select :open @20:25:07.09 SS.seek 0ml @20:25:15.94 Exhaust.select :close @20:25:16.05 SSV.dial CBV @20:25:16.08 CBV.dial SSV @20:25:16.85 Allowing 130s for 25.0ml of remaining 25.0ml @20:25:16.86 Intake.select :open @20:25:19.03 Intake @15.6psia, vacuum from 10.0 to 13.0psi @20:25:19.05 SS.reconfigure :inhaling 2.20ml/s @20:25:19.10 SS.seek 25ml @20:25:34.73 Stroke completed -- waiting for pressure to equalize ... @20:25:34.75 SS.reconfigure :exhale @20:25:34.79 SS.hold @20:25:40.45 Intake.select :close @20:25:40.61 SSV.dial :exhaust @20:25:41.39 Exhaust.select :open @20:25:41.95 SS.seek 0ml @20:25:50.84 Exhaust.select :close @20:25:50.95 Sampled 50.0ml @20:25:50.97 Drying puck before fixing for 5:00 @20:25:50.98 CBV.dial :waste2b @20:25:51.75 delay 1:00 @20:26:51.78 CSV.dial :air @20:26:52.25 CS.reconfigure :fast @20:26:52.31 CS.seek 10ml @20:27:17.42 Pumping wcr puck from tube 2 up to 35psi... @20:27:17.44 CBV.dial SSV @20:27:18.71 CBV.dial between CSV and waste2b @20:27:19.68 CSV.dial between air and CTV @20:27:20.02 CS.seek 7.328ml @20:27:27.11 CTV.dial CSV,avoiding: :air @20:27:27.14 CSV.dial CTV @20:27:27.97 CS.reconfigure :pressurize @20:27:28.02 CS.seek 0ml @20:27:44.57 CBV.dial :waste2b @20:27:44.89 CS.reconfigure :default @20:27:44.94 CS.seek 0ml @20:27:48.32 delay 1:00 @20:27:55.24 recoverPRVreagent08 @20:27:55.25 PTV.dial :mixing @20:27:56.02 PBV.dial :waste1 @20:27:56.38 delay 30 seconds @20:28:26.41 PBV.dial between PSV and waste1 @20:28:26.77 PSV.dial :air @20:28:27.24 PS.reconfigure :fast @20:28:27.29 PS.seek 2.5ml @20:28:48.35 CTV.dial :air,avoiding: :intake @20:28:49.69 Fix #1 of 2 (with RNAlater) for 2:30 @20:28:49.71 CSV.dial CSR @20:28:50.14 Pulling 2ml of CSR.RNAlater @20:28:50.15 CSR.select :RNAlater @20:28:50.27 CS.seek 2ml @20:28:51.48 PSV.dial :mixloop @20:28:51.96 delay 2 seconds @20:28:53.97 PSV.dial :air @20:28:54.44 delay 2 seconds @20:28:56.26 delay 5 seconds @20:28:56.46 PSV.dial :mixloop @20:28:56.93 delay 2 seconds @20:28:58.94 PSV.dial :air @20:28:59.42 PS.seek 0ml @20:29:01.28 CSR.select :air @20:29:01.39 CS.reconfigure :fast @20:29:01.88 CS.seek 10ml @20:29:22.04 Pumping wcr puck from tube 2 up to 35psi... @20:29:22.06 CBV.dial SSV @20:29:22.86 washCycleDA #1 of 2 @20:29:22.87 PSR.select :air @20:29:22.89 PSV.dial PSR @20:29:23.33 CBV.dial between CSV and waste2b @20:29:24.25 Pulling 0.5ml of PSR.wash @20:29:24.29 PSR.select :wash @20:29:24.40 CSV.dial between air and CTV @20:29:24.47 PS.reconfigure :default @20:29:24.52 PS.seek 0.5ml @20:29:25.00 CS.seek 7.293ml @20:29:30.45 delay 5 seconds @20:29:32.11 CTV.dial CSV,avoiding: :air @20:29:32.13 CSV.dial CTV @20:29:33.50 CS.reconfigure :pressurize @20:29:33.56 CS.seek 0ml @20:29:35.47 PSR.select :air @20:29:35.60 PS.seek 2ml @20:29:48.19 delay 2:30 @20:29:51.73 PTV.dial :puck @20:29:52.49 PSV.dial PTV @20:29:53.26 PBV.dial :waste1 @20:29:53.67 PS.seek 1.5ml @20:29:59.58 delay 3 seconds @20:30:02.60 PBV.dial between PSV and waste1 @20:30:02.96 PS.seek 0ml @20:30:19.27 delay 2:00 @20:32:18.22 CBV.dial :waste2b @20:32:18.56 CS.reconfigure :default @20:32:18.61 CS.seek 0ml @20:32:19.29 recoverPSRreagent08 @20:32:19.30 PTV.dial :puck @20:32:19.37 PBV.dial :waste1 @20:32:19.73 delay 30 seconds @20:32:24.07 delay 1:00 @20:32:49.76 PBV.dial between PSV and waste1 @20:32:50.12 PSR.select :air @20:32:50.14 PSV.dial PSR @20:32:50.91 PS.reconfigure :fast @20:32:50.96 PS.seek 2.5ml @20:33:15.23 PSV.dial PTV @20:33:16.12 delay 5 seconds @20:33:21.14 PSV.dial PSR @20:33:22.01 delay 5 seconds @20:33:24.09 CTV.dial :air,avoiding: :intake @20:33:25.42 Fix #2 of 2 (with RNAlater) for 2:30 @20:33:25.43 CSV.dial CSR @20:33:25.87 Pulling 2ml of CSR.RNAlater @20:33:26.30 CSR.select :RNAlater @20:33:26.45 CS.seek 2ml @20:33:27.03 PSV.dial PTV @20:33:27.78 delay 5 seconds @20:33:32.44 delay 5 seconds @20:33:32.80 PSV.dial :air @20:33:33.69 PS.seek 0ml @20:33:37.46 CSR.select :air @20:33:37.58 CS.reconfigure :fast @20:33:37.64 CS.seek 10ml @20:33:57.36 washCycleDA #2 of 2 @20:33:57.37 PSR.select :air @20:33:57.38 PSV.dial PSR @20:33:57.63 Pumping wcr puck from tube 2 up to 35psi... @20:33:57.65 CBV.dial SSV @20:33:58.73 Pulling 0.5ml of PSR.wash @20:33:58.74 PSR.select :wash @20:33:58.85 PS.reconfigure :default @20:33:58.90 PS.seek 0.5ml @20:33:58.95 CBV.dial between CSV and waste2b @20:33:59.92 CSV.dial between air and CTV @20:34:00.53 CS.seek 7.28ml @20:34:04.85 delay 5 seconds @20:34:07.64 CTV.dial CSV,avoiding: :air @20:34:07.69 CSV.dial CTV @20:34:09.03 CS.reconfigure :pressurize @20:34:09.08 CS.seek 0ml @20:34:09.87 PSR.select :air @20:34:09.99 PS.seek 2ml @20:34:23.65 delay 2:30 @20:34:26.20 PTV.dial :puck @20:34:26.28 PSV.dial PTV @20:34:27.04 PBV.dial :waste1 @20:34:27.48 PS.seek 1.5ml @20:34:33.38 delay 3 seconds @20:34:36.40 PBV.dial between PSV and waste1 @20:34:36.78 PS.seek 0ml @20:34:52.95 delay 2:00 @20:36:52.97 recoverPSRreagent08 @20:36:52.98 PTV.dial :puck @20:36:53.06 PBV.dial :waste1 @20:36:53.43 delay 30 seconds @20:36:53.67 CBV.dial :waste2b @20:36:54.02 CS.reconfigure :default @20:36:54.08 CS.seek 0ml @20:36:59.38 delay 1:00 @20:37:23.45 PBV.dial between PSV and waste1 @20:37:23.82 PSR.select :air @20:37:23.84 PSV.dial PSR @20:37:25.04 PS.reconfigure :fast @20:37:25.09 PS.seek 2.5ml @20:37:49.22 PSV.dial PTV @20:37:50.12 delay 5 seconds @20:37:55.14 PSV.dial PSR @20:37:55.88 delay 5 seconds @20:37:59.40 CTV.dial :air,avoiding: :intake @20:38:00.90 PSV.dial PTV @20:38:01.66 delay 5 seconds @20:38:06.68 PSV.dial :air @20:38:07.46 PS.seek 0ml @20:38:30.97 getPMLReagent08 @20:38:30.98 PRV.dial :air @20:38:31.44 delay 5 seconds @20:38:36.46 PTV.dial :PRVmixing @20:38:37.82 delay 5 seconds @20:38:42.84 PRV.dial :ab2 @20:38:43.30 PTV.dial :PRVmixing @20:38:43.49 PSV.dial :mixloop @20:38:43.98 PS.reconfigure :default @20:38:44.03 PS.seek 0.3ml @20:38:47.89 delay 2 seconds @20:38:49.90 PRV.dial :air @20:38:50.44 PS.seek 0.55ml @20:38:53.79 addPMLReagent08 @20:38:53.80 PSV.dial :air @20:38:54.26 PS.seek 2ml @20:39:09.91 PSV.dial :PML @20:39:10.38 PTV.dial :mixing @20:39:11.14 PBV.dial :waste1 @20:39:11.58 PS.seek 1.85ml @20:39:13.97 PBV.dial between PSV and waste1 @20:39:14.35 PS.seek 0ml @20:39:34.03 delay 2 seconds @20:39:36.05 delay 8:00 @20:47:36.08 recoverPRVreagent08 @20:47:36.09 PTV.dial :mixing @20:47:36.16 PBV.dial :waste1 @20:47:36.54 delay 30 seconds @20:48:06.56 PBV.dial between PSV and waste1 @20:48:06.92 PSV.dial :air @20:48:07.40 PS.reconfigure :fast @20:48:07.45 PS.seek 2.5ml @20:48:31.55 PSV.dial :mixloop @20:48:32.02 delay 2 seconds @20:48:34.04 PSV.dial :air @20:48:34.50 delay 2 seconds @20:48:36.52 PSV.dial :mixloop @20:48:36.99 delay 2 seconds @20:48:39.01 PSV.dial :air @20:48:39.48 PS.seek 0ml @20:49:03.02 washCycleDA #1 of 3 @20:49:03.03 PSR.select :air @20:49:03.05 PSV.dial PSR @20:49:04.39 Pulling 0.5ml of PSR.wash @20:49:04.40 PSR.select :wash @20:49:04.52 PS.reconfigure :default @20:49:04.57 PS.seek 0.5ml @20:49:10.58 delay 5 seconds @20:49:15.60 PSR.select :air @20:49:16.08 PS.seek 2ml @20:49:32.27 PTV.dial :puck @20:49:33.02 PSV.dial PTV @20:49:33.77 PBV.dial :waste1 @20:49:34.19 PS.seek 1.5ml @20:49:40.14 delay 3 seconds @20:49:43.16 PBV.dial between PSV and waste1 @20:49:43.52 PS.seek 0ml @20:49:59.67 delay 2:00 @20:51:59.70 recoverPSRreagent08 @20:51:59.71 PTV.dial :puck @20:51:59.87 PBV.dial :waste1 @20:52:00.23 delay 30 seconds @20:52:30.26 PBV.dial between PSV and waste1 @20:52:30.62 PSR.select :air @20:52:30.64 PSV.dial PSR @20:52:31.41 PS.reconfigure :fast @20:52:31.46 PS.seek 2.5ml @20:52:55.42 PSV.dial PTV @20:52:56.18 delay 5 seconds @20:53:01.21 PSV.dial PSR @20:53:01.97 delay 5 seconds @20:53:06.99 PSV.dial PTV @20:53:07.75 delay 5 seconds @20:53:12.77 PSV.dial :air @20:53:13.53 PS.seek 0ml @20:53:37.14 washCycleDA #2 of 3 @20:53:37.15 PSR.select :air @20:53:37.16 PSV.dial PSR @20:53:38.51 Pulling 0.5ml of PSR.wash @20:53:38.52 PSR.select :wash @20:53:38.63 PS.reconfigure :default @20:53:38.68 PS.seek 0.5ml @20:53:44.57 delay 5 seconds @20:53:49.60 PSR.select :air @20:53:49.71 PS.seek 2ml @20:54:05.85 PTV.dial :puck @20:54:05.86 PSV.dial PTV @20:54:06.62 PBV.dial :waste1 @20:54:07.04 PS.seek 1.5ml @20:54:12.91 delay 3 seconds @20:54:15.93 PBV.dial between PSV and waste1 @20:54:16.30 PS.seek 0ml @20:54:32.38 delay 2:00 @20:56:32.41 recoverPSRreagent08 @20:56:32.42 PTV.dial :puck @20:56:32.43 PBV.dial :waste1 @20:56:32.79 delay 30 seconds @20:57:02.81 PBV.dial between PSV and waste1 @20:57:03.18 PSR.select :air @20:57:03.19 PSV.dial PSR @20:57:04.09 PS.reconfigure :fast @20:57:04.14 PS.seek 2.5ml @20:57:28.41 PSV.dial PTV @20:57:29.16 delay 5 seconds @20:57:34.18 PSV.dial PSR @20:57:34.94 delay 5 seconds @20:57:39.96 PSV.dial PTV @20:57:40.72 delay 5 seconds @20:57:45.74 PSV.dial :air @20:57:46.51 PS.seek 0ml @20:58:09.97 washCycleDA #3 of 3 @20:58:09.98 PSR.select :air @20:58:10.00 PSV.dial PSR @20:58:11.82 Pulling 0.5ml of PSR.wash @20:58:11.83 PSR.select :wash @20:58:11.95 PS.reconfigure :default @20:58:12.00 PS.seek 0.5ml @20:58:17.91 delay 5 seconds @20:58:22.93 PSR.select :air @20:58:23.05 PS.seek 2ml @20:58:39.31 PTV.dial :puck @20:58:39.33 PSV.dial PTV @20:58:40.08 PBV.dial :waste1 @20:58:40.52 PS.seek 1.5ml @20:58:46.56 delay 3 seconds @20:58:49.58 PBV.dial between PSV and waste1 @20:58:49.96 PS.seek 0ml @20:59:06.28 delay 2:00 @21:01:06.31 recoverPSRreagent08 @21:01:06.32 PTV.dial :puck @21:01:06.33 PBV.dial :waste1 @21:01:06.70 delay 30 seconds @21:01:36.73 PBV.dial between PSV and waste1 @21:01:37.11 PSR.select :air @21:01:37.13 PSV.dial PSR @21:01:37.89 PS.reconfigure :fast @21:01:37.94 PS.seek 2.5ml @21:02:02.00 PSV.dial PTV @21:02:02.76 delay 5 seconds @21:02:07.78 PSV.dial PSR @21:02:08.54 delay 5 seconds @21:02:13.57 PSV.dial PTV @21:02:14.34 delay 5 seconds @21:02:19.36 PSV.dial :air @21:02:20.13 PS.seek 0ml @21:02:43.65 Gate.power :camera,:ON @21:02:43.67 Mixing DA Substrates @21:02:43.68 PSR.select :air @21:02:43.69 PSV.dial PSR @21:02:45.09 Pulling 0.25ml of PSR.sub1 @21:02:45.11 PSR.select :sub1 @21:02:45.31 PS.reconfigure :default @21:02:45.42 PS.seek 0.25ml @21:02:49.04 delay 5 seconds @21:02:54.06 Pulling 0.25ml of PSR.sub2 @21:02:54.07 PSR.select :sub2 @21:02:54.19 PS.seek 0.5ml @21:02:57.62 delay 5 seconds @21:03:02.64 PSR.select :air @21:03:02.75 PS.seek 2ml @21:03:18.84 PTV.dial :puck @21:03:18.85 PSV.dial PTV @21:03:19.62 PBV.dial :waste1 @21:03:20.06 PS.seek 1.5ml @21:03:25.96 delay 3 seconds @21:03:28.98 PBV.dial between PSV and waste1 @21:03:29.36 PS.seek 0ml @21:03:45.56 delay 10 seconds @21:03:55.59 recoverPSRreagent08 PTV.dial :puck @21:03:55.60 PBV.dial :waste1 @21:03:55.98 delay 30 seconds @21:04:26.01 PBV.dial between PSV and waste1 @21:04:26.38 PSR.select :air @21:04:26.40 PSV.dial PSR @21:04:27.31 PS.reconfigure :fast @21:04:27.36 PS.seek 2.5ml @21:04:51.83 PSV.dial PTV @21:04:52.60 delay 5 seconds @21:04:58.04 PSV.dial PSR @21:04:58.85 delay 5 seconds @21:05:03.87 PSV.dial PTV @21:05:04.65 delay 5 seconds @21:05:09.67 PSV.dial :air @21:05:10.44 PS.seek 0ml @21:05:34.56 Additional air clear @21:05:34.57 PSR.select :air @21:05:34.58 PSV.dial PSR @21:05:35.94 Pulling 2ml of PSR.air @21:05:35.95 PSR.select :air @21:05:35.98 PS.reconfigure :default @21:05:36.02 PS.seek 2ml @21:05:57.46 delay 5 seconds @21:06:02.48 PTV.dial :puck @21:06:02.50 PSV.dial PTV @21:06:03.26 PBV.dial :waste1 @21:06:03.69 PS.seek 1.5ml @21:06:09.68 delay 3 seconds @21:06:12.70 PBV.dial between PSV and waste1 @21:06:13.07 delay 1 second @21:06:14.09 PSV.dial :waste1 @21:06:15.16 PS.seek 0ml @21:06:31.36 Processing Puck Heater at 28C before imaging @21:06:31.37 PH.coast @21:06:31.48 Imaging da puck in Processing Clamp while storing it to tube 1 @21:06:31.65 SC.reconfigure :tight @21:06:31.70 SC.seek 1 @21:06:35.33 SC.configure :limp @21:06:35.55 Elbow.reconfigure :tight @21:06:35.60 Elbow.seek 1 @21:06:37.35 Forearm.reconfigure :tight @21:06:37.40 Forearm.seek 1 + 450 counts @21:06:38.43 SE.reconfigure :pick @21:06:38.49 SE.seek :beamBreak @21:06:41.54 SE.configure :default @21:06:41.70 Elbow.reconfigure :limp @21:06:41.75 Forearm.reconfigure :loose @21:06:41.80 Forearm.seek 1 @21:06:42.07 Elbow.hold @21:06:42.14 Elbow.reconfigure :tight @21:06:42.25 SE.seek 1.18 Pucks below Top Plate @21:06:42.83 Forearm.force -20 @21:06:42.87 Forearm.seek 1 + 302 counts @21:06:43.73 Forearm.reconfigure :tight @21:06:43.78 Forearm.seek 1 + 450 counts @21:06:43.98 SE.seek 2.18 Pucks below Top Plate @21:06:44.64 Forearm.reconfigure :loose @21:06:44.69 Forearm.seek :retract @21:06:45.62 Elbow.seek PC @21:06:48.11 Elbow.reconfigure :loose @21:06:48.21 Forearm.seek PC @21:06:49.15 Hand.close @21:06:49.87 PC.seek :open @21:06:54.66 Forearm.seek :retract @21:06:55.64 Elbow.reconfigure :tight @21:06:55.69 Elbow.seek 1 @21:06:58.28 Forearm.reconfigure :tight @21:06:58.33 Forearm.seek 1 + 450 counts @21:06:59.30 Hand.open @21:06:59.93 SE.seek 1.83 Pucks below Top Plate @21:07:00.22 SE.reconfigure :seal @21:07:00.27 SE.seek 0.86 Pucks below Top Plate @21:07:00.77 Lights, camera, ACTION! @21:07:00.81 PuckCamera: snap -bin=2,2 -auto /var/log/esp/da24jan1021h50ml.tif @21:07:08.14 SX HX9/16 (USB:02): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 2.811 seconds /var/log/esp/da24jan1021h50ml.tif: TIFF Upload Complete @21:07:08.17 PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da24jan1021h50ml15s.tif @21:07:20.33 Waiting >20 seconds for da to relinquish FlushPuck @21:07:23.81 SX HX9/16 (USB:02): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15 seconds /var/log/esp/midres/da24jan1021h50ml15s.tif: TIFF Upload Complete @21:07:23.84 PuckCamera: snap -bin=2,2 20.0 /var/log/esp/midres/da24jan1021h50ml20s.tif @21:07:44.45 SX HX9/16 (USB:02): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 20 seconds /var/log/esp/midres/da24jan1021h50ml20s.tif: TIFF Upload Complete @21:07:44.47 DAfans.email "2.811 sec auto-exposure",Subject: "da24jan1021h50ml images!" @21:07:44.50 Gate.power :camera,:OFF @21:07:44.93 Elbow.seek 1 @21:07:45.16 Forearm.seek 1 + 450 counts @21:07:45.36 SE.reconfigure :gently @21:07:45.41 SE.seek 1.58 Pucks below Top Plate @21:07:46.08 Elbow.reconfigure :limp @21:07:46.15 Forearm.reconfigure :loose @21:07:46.78 Forearm.seek 1 @21:07:47.02 Elbow.hold @21:07:47.09 Elbow.reconfigure :tight @21:07:47.17 SE.reconfigure :default @21:07:47.23 SE.seek 1.76 Pucks below Top Plate @21:07:47.58 Forearm.force -20 @21:07:47.63 Forearm.seek 1 + 306 counts @21:07:48.02 Forearm.reconfigure :tight @21:07:48.07 Forearm.seek 1 + 450 counts @21:07:48.66 SE.seek :down @21:07:49.99 Forearm.reconfigure :loose @21:07:50.09 Forearm.seek :retract @21:07:51.26 SE.stop @21:07:51.51 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @21:07:52.05 Elbow.seek FlushPuck::Garage @21:07:53.99 Elbow.reconfigure :loose @21:07:54.24 Forearm.seek :garage @21:07:55.31 Hand.close @21:07:56.53 Forearm.seek :retract @21:07:58.11 Elbow.reconfigure :tight @21:07:58.22 Elbow.seek PC @21:07:59.60 Elbow.reconfigure :loose @21:07:59.86 Forearm.reconfigure :tight @21:07:59.95 Forearm.seek PC @21:08:01.75 PC.seek :closed @21:08:05.31 upsync @21:08:06.43 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.63 @21:08:06.50 Hand.open @21:08:07.18 Forearm.reconfigure :loose @21:08:07.23 Forearm.seek :retract @21:08:08.23 PSR.select :air @21:08:08.26 Storing wcr puck in Collection Clamp to tube 1 @21:08:08.28 PSV.dial PSR @21:08:08.76 Elbow.reconfigure :tight @21:08:08.83 Elbow.seek 1 @21:08:08.85 Pulling 0.5ml of PSR.flush @21:08:08.88 PSR.select :flush @21:08:09.05 PS.seek 0.5ml @21:08:11.51 Forearm.reconfigure :tight @21:08:11.56 Forearm.seek 1 + 450 counts @21:08:12.60 SE.reconfigure :pick @21:08:12.66 SE.seek :beamBreak @21:08:15.05 delay 5 seconds @21:08:15.63 SE.configure :default @21:08:15.80 Elbow.reconfigure :limp @21:08:15.85 Forearm.reconfigure :loose @21:08:15.90 Forearm.seek 1 @21:08:16.19 Elbow.hold @21:08:16.26 Elbow.reconfigure :tight @21:08:16.37 SE.seek 2.16 Pucks below Top Plate @21:08:16.85 Forearm.force -20 @21:08:16.90 Forearm.seek 1 + 292 counts @21:08:17.34 Forearm.reconfigure :tight @21:08:17.39 Forearm.seek 1 + 450 counts @21:08:18.03 SE.seek 3.16 Pucks below Top Plate @21:08:18.55 Forearm.reconfigure :loose @21:08:18.60 Forearm.seek :retract @21:08:19.54 Elbow.seek CC @21:08:20.07 PSR.select :air @21:08:20.18 PS.seek 2.5ml @21:08:21.16 Elbow.reconfigure :loose @21:08:21.27 Forearm.seek CC @21:08:22.23 Hand.close @21:08:22.87 CC.seek :open @21:08:27.64 Forearm.seek :retract @21:08:28.64 Elbow.reconfigure :tight @21:08:28.69 Elbow.seek 1 @21:08:30.50 Elbow.reconfigure :limp @21:08:30.55 Forearm.seek 1 @21:08:31.55 Elbow.hold @21:08:31.62 Elbow.reconfigure :tight @21:08:31.67 Hand.open @21:08:32.37 Forearm.force -20 @21:08:32.42 Forearm.seek 1 + 301 counts @21:08:32.83 Forearm.reconfigure :tight @21:08:32.89 Forearm.seek 1 + 450 counts @21:08:33.17 SE.seek :down @21:08:34.68 Forearm.reconfigure :loose @21:08:34.80 Forearm.seek :retract @21:08:35.69 SE.stop @21:08:35.76
Starting finalPurge @21:08:41.36 delay 1 second @21:08:42.38 PRV.dial :waste2,avoiding: :ab2 @21:08:44.24 PTV.dial :PRVmixing @21:08:45.55 PSV.dial :mixloop @21:08:46.59 PS.seek :empty @21:08:55.69
Waiting >20 seconds for psFlush to relinquish FlushPuck @21:09:13.02 PSR.select :air @21:09:13.04 PSV.dial PSR @21:09:14.08 Pulling 0.5ml of PSR.flush @21:09:14.09 PSR.select :flush @21:09:14.21 PS.seek 0.5ml @21:09:20.14 delay 5 seconds @21:09:25.16 PSR.select :air @21:09:25.28 PS.seek 2.5ml @21:09:46.58 delay 1 second @21:09:47.60 PBV.dial :waste1 @21:09:47.95 PTV.dial :mixing @21:09:48.70 PSV.dial :mixloop @21:09:49.75 PS.seek :empty @21:10:16.11 PSV.dial PSR @21:10:17.16 PSR.select :flush @21:10:17.28 PS.seek 1ml @21:10:28.39 PSR.select :air @21:10:28.50 PS.seek 2.5ml @21:10:44.67 PSV.dial PTV @21:10:45.44 PTV.dial :puck @21:10:46.17 PBV.dial :waste1 @21:10:46.19 PS.seek 0ml @21:11:12.54 PSV.dial PSR @21:11:13.42 PSR.select :flush @21:11:13.54 PS.seek 0.5ml @21:11:19.48 PSR.select :air @21:11:19.60 PS.seek 2.5ml @21:11:40.89 PSV.dial PTV @21:11:41.62 PBV.dial PSV @21:11:42.18 PS.seek 0ml @21:12:08.51 PSV.dial PBV @21:12:08.99 PS.seek 2.5ml @21:12:35.30 PSV.dial :waste1 @21:12:36.04 PS.seek 0ml @21:13:02.45 PSV.dial PSR @21:13:02.92 PSR.select :air @21:13:02.95 PS.seek 2.5ml @21:13:29.36 PSV.dial PTV @21:13:30.09 PBV.dial PSV @21:13:30.61 PS.seek 0ml @21:13:57.01 PSV.dial PBV @21:13:57.49 PS.seek 2.5ml @21:14:23.86 PSV.dial :waste1 @21:14:24.72 PS.seek 0ml @21:14:51.01 PSV.dial PSR @21:14:51.47 PSR.select :flush @21:14:51.59 PS.seek 1ml @21:15:02.67 PSR.select :air @21:15:02.78 PS.seek 2.5ml @21:15:18.98 PSV.dial PTV @21:15:19.84 PTV.dial :puck @21:15:19.92 PBV.dial :waste1 @21:15:20.47 PS.seek 0ml @21:15:46.89 PSV.dial PSR @21:15:47.64 PSR.select :flush @21:15:47.76 PS.seek 0.5ml @21:15:53.73 PSR.select :air @21:15:53.85 PS.seek 2.5ml @21:16:15.17 PSV.dial PTV @21:16:15.92 PBV.dial PSV @21:16:16.49 PS.seek 0ml @21:16:43.01 PSV.dial PBV @21:16:43.49 PS.seek 2.5ml @21:17:09.85 PSV.dial :waste1 @21:17:10.60 PS.seek 0ml @21:17:37.13 PSV.dial PSR @21:17:37.60 PSR.select :air @21:17:37.63 PS.seek 2.5ml @21:18:03.95 PSV.dial PTV @21:18:04.68 PBV.dial PSV @21:18:04.88 PS.seek 0ml @21:18:31.23 PSV.dial PBV @21:18:31.70 PS.seek 2.5ml @21:18:58.10 PSV.dial :waste1 @21:18:58.84 PS.seek 0ml @21:19:25.23
Loading FlushPuck into Collection Clamp from Processing Clamp @21:19:25.42 Elbow.seek PC @21:19:27.92 Elbow.reconfigure :loose @21:19:28.02 Forearm.seek PC @21:19:29.41 Hand.close @21:19:29.99 PC.seek :open @21:19:34.72 Forearm.seek :retract @21:19:35.68 Elbow.reconfigure :tight @21:19:35.72 Elbow.seek CC @21:19:39.36 Elbow.reconfigure :loose @21:19:39.46 Forearm.reconfigure :tight @21:19:39.51 Forearm.seek CC @21:19:40.80 CC.seek :closed @21:19:45.33 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.37 @21:19:45.40 Hand.open @21:19:46.05 Forearm.reconfigure :loose @21:19:46.09 Forearm.seek :retract @21:19:47.13 Flushing the Collection Valve::Manifold @21:19:47.25 CSV.dial CSR @21:19:47.70 Pulling 5ml of CSR.flush @21:19:47.71 CSR.select :flush @21:19:47.83 CS.seek 5ml @21:20:01.39 delay 5 seconds @21:20:06.41 CSR.select :air @21:20:06.58 CS.reconfigure :fast @21:20:06.63 CS.seek 7.5ml @21:20:13.48 CSV.dial :waste @21:20:14.77 CS.reconfigure :default @21:20:14.81 CS.seek 0ml @21:20:34.86 Flushing lower manifold's waste1 port @21:20:34.98 CSV.dial :flush @21:20:35.42 CS.reconfigure :fast @21:20:35.47 CS.seek 5ml @21:20:48.67 delay 5 seconds @21:20:53.69 CSV.dial :air @21:20:54.31 CS.seek 7ml @21:21:00.42 CSV.dial CTV @21:21:00.45
CTV.dial CSV @21:21:01.82 CBV.dial :waste1 @21:21:02.60 CS.seek 0ml @21:21:19.45 Flushing lower manifold's waste2 port @21:21:19.57 CSV.dial :flush @21:21:20.31 CS.seek 5ml @21:21:33.14 delay 5 seconds @21:21:38.16 CSV.dial :air @21:21:38.67 CS.seek 7ml @21:21:44.29 CSV.dial CTV @21:21:44.32
CTV.dial CSV @21:21:44.77 CBV.dial :waste2 @21:21:45.81 CS.seek 0ml @21:22:02.60 Flushing lower manifold's waste2b port @21:22:02.73 CSV.dial :flush @21:22:03.47 CS.seek 5ml @21:22:16.26 delay 5 seconds @21:22:21.28 CSV.dial :air @21:22:21.81 CS.seek 7ml @21:22:27.64 CSV.dial CTV @21:22:27.67
CTV.dial CSV @21:22:28.10 CBV.dial :waste2b @21:22:29.14 CS.seek 0ml @21:22:45.76 Disinfecting external Intake with kill @21:22:45.88 CSV.dial CSR @21:22:46.32 Pulling 8ml of CSR.kill @21:22:46.33 CSR.select :kill @21:22:46.45 CS.seek 8ml @21:23:06.54 delay 5 seconds @21:23:11.56 CSR.select :air @21:23:11.68 CS.seek 10ml @21:23:17.29 CSV.dial CBV @21:23:17.31
CBV.dial CSV @21:23:18.38 CTV.dial :intake @21:23:19.16 Intake.select :open @21:23:19.28 CS.seek 2ml @21:23:38.22 Intake.select :close @21:23:38.33 CTV.dial :air @21:23:39.06 CS.reconfigure :default @21:23:39.11 CS.seek 0ml @21:23:45.04 CSV.dial CSR @21:23:46.05 Pulling 5ml of CSR.flush @21:23:46.06 CSR.select :flush @21:23:46.18 CS.seek 5ml @21:23:59.88 delay 5 seconds @21:24:04.90 CSR.select :air @21:24:05.02 CS.seek 10ml @21:24:18.89 CSV.dial CBV @21:24:19.89 CS.seek 0ml @21:24:46.32 Garaging FlushPuck in Collection Clamp @21:24:46.39 Elbow.reconfigure :tight @21:24:46.44 Elbow.seek CC @21:24:46.68 Elbow.reconfigure :loose @21:24:46.78 Forearm.seek CC @21:24:47.79 Hand.close @21:24:48.36 CC.seek :open @21:24:53.03 Forearm.seek :retract @21:24:54.00 Elbow.reconfigure :tight @21:24:54.05 Elbow.seek :garage @21:24:56.95 Elbow.reconfigure :loose @21:24:57.05 Forearm.reconfigure :tight @21:24:57.09 Forearm.seek :garage @21:24:58.10 Hand.open @21:24:58.79 Forearm.reconfigure :loose @21:24:58.84 Forearm.seek :retract @21:24:59.83 Completed [initialPurge; da [50, 50] #da and wsr are run simultanelously] begun 19:34:26 @21:24:59.88 Asleep after completing DA_WCR_011024 assays @21:24:59.90 GoodNews.email "{1 => 4,\n 2 => 0,\n 3 => 0,\n 4 => 0,\n 5 => 0,\n 6 => 0,\n 7 => 0}\nCTD data unavailable\nCan@21:23:36, 25% flow, 20.9C, 25% humidity, 14.6psia, 13.627V, 0.580A, 1.306Ah, 7.91W\nSampled 50.0ml",Subject: "Asleep after completing DA_WCR_011024 assays" @21:25:04.56 upsync + ESPwhich DA_WCR_011024.rb + fn=DA_WCR_011024.rb + IFS=: + set /home/esp/esp2/mission . + fullPath=/home/esp/esp2/mission/DA_WCR_011024.rb + [ -f /home/esp/esp2/mission/DA_WCR_011024.rb ] + realpath /home/esp/esp2/mission/DA_WCR_011024.rb + return 0 + scriptPath=/home/esp/esp2local/mission/DA_WCR_011024.rb + exec ruby /home/esp/esp2/lib/esp.rb /home/esp/esp2local/mission/DA_WCR_011024.rb @15:27:01.68UTC12-Jan-24
ESP2 v3.42 I2C Gateway v3.20 for core bus @15:27:04.31 Configuration for short puck jake 1/11/21 by brent@mbari.org /home/esp/esp2/type/shallow/jake/configure.rb @15:27:07.86 Polling Can every 10:00 @15:27:07.88 Cleanly exited at 15:09:41UTC12-Jan-24 @15:27:07.93 Loading /home/esp/esp2local/mission/DA_WCR_011024.rb mission @15:27:07.99 GoodNews.email "Starting DA_WCR_011024 w/pucks in tube 2\n{3 => 0,\n 4 => 0,\n 5 => 0,\n 6 => 0,\n 7 => 0}\nCTD data unavailable\nCan@15:27:07, 26% flow, 21.7C, 22% humidity, 15.0psia, 13.798V, 0.315A, 13.872Ah, 4.34W",Subject: "DA_WCR_011024 begun" @15:27:08.02
Preparing to sleep until + 00:00:05 @15:27:08.07 Asleep til + 00:00:05 @15:27:08.09 Continuing at 15:27 @15:27:08.37 Gate.power :core,:OFF @15:27:08.39 Gate.power :raw,:OFF @15:27:08.40 Shutdown @15:27:10.88 GoodNews.email "Then, in tube 2: initialPurge; da [50, 50] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_011024.rb:14\n{1 => 0,\n 2 => 4,\n 3 => 0,\n 4 => 0,\n 5 => 0,\n 6 => 0,\n 7 => 0}\nCTD data unavailable\nCan@15:27:08, 26% flow, 21.7C, 22% humidity, 15.0psia, 13.798V, 0.315A, 13.872Ah, 4.34W",Subject: "Asleep until 15:27" @15:27:12.67 upsync @15:27:12.97
Powering core bus @15:27:14.66 upsync @15:27:18.58
Gate.power :raw,:ON @15:27:19.53 CS.configure :default @15:27:19.58 PS.configure :default @15:27:19.63 SS.configure :default @15:27:19.67 CC.configure :default @15:27:19.73 PC.configure :default @15:27:19.80 Forearm.configure :loose @15:27:19.85 Elbow.configure :loose @15:27:19.92 SC.configure :loose @15:27:19.97 SE.configure :default @15:27:20.02 CH.configure :default @15:27:20.05 PH.configure :default @15:27:20.09 All dwarf controllers running firmware version 3.85 @15:27:20.20 Valve.reset @15:27:20.42 Intake.select :close @15:27:20.48 Exhaust.select :close @15:27:20.51 CTV.dial :air @15:27:20.59 PSV.dial :air @15:27:20.73 CSV.dial :waste1 @15:27:20.76
Forearm.home.seek :retract @15:27:21.20 SSV.dial :spit,avoiding: :exhaust @15:27:21.49 Hand.close @15:27:21.53
Elbow.reconfigure :loose @15:27:21.61 Elbow.jog 1500 @15:27:21.68 PS.home.seek 0ml @15:27:22.05 CS.reconfigure :fast @15:27:22.09 SS.home.seek 0ml @15:27:22.23 CS.home.seek 0ml @15:27:22.24
Elbow.reconfigure :loose @15:27:22.33 Elbow.home.seek :clear @15:27:25.52 SE.home.seek :down @15:27:26.14 SC.reconfigure :tight @15:27:26.19 SC.home.seek :initial @15:27:29.99 PS.stop @15:27:51.35
SC.configure :limp @15:27:51.40 Forearm.seek :retract @15:27:51.55 Elbow.seek :hammer @15:27:52.68 Forearm.reconfigure :tight @15:27:52.72 Forearm.seek retracted @15:27:52.99 CC.home.seek :open @15:27:53.00 PC.home.seek :open @15:27:56.56
Phase [initialPurge; da [50, 50] #da and wsr are run simultanelously] @15:27:56.58 Notice.email "At 15:27:56 began in tube 2: initialPurge; da [50, 50] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_011024.rb:14\n{1 => 0,\n 2 => 4,\n 3 => 0,\n 4 => 0,\n 5 => 0,\n 6 => 0,\n 7 => 0}\nCTD data unavailable\nCan@15:27:56, 26% flow, 21.9C, 22% humidity, 15.0psia, 13.749V, 0.321A, 13.877Ah, 4.41W",Subject: "Started DA_WCR_011024 phase initialPurge; da [50, 50] #da and wsr are run simultanelously" @15:27:56.60
Starting initialPurge @15:27:56.62 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @15:27:56.69 Hand.open @15:27:57.40 Elbow.reconfigure :tight @15:27:57.45 Elbow.seek FlushPuck::Garage @15:27:59.40 Elbow.reconfigure :loose @15:27:59.55 Forearm.reconfigure :loose @15:27:59.60 Forearm.seek :garage @15:28:00.53 upsync @15:28:00.63
Hand.close @15:28:01.40 Forearm.seek :retract @15:28:02.41 Elbow.reconfigure :tight @15:28:02.46 Elbow.seek CC @15:28:05.35 Elbow.reconfigure :loose @15:28:05.45 Forearm.reconfigure :tight @15:28:05.50 Forearm.seek CC @15:28:06.79 CC.seek :closed @15:28:11.42 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.38 @15:28:11.49 Hand.open @15:28:12.22 Forearm.reconfigure :loose @15:28:12.26 Forearm.seek :retract @15:28:13.24 Flushing the Collection Valve::Manifold @15:28:13.36 CSV.dial CSR @15:28:14.75 Pulling 5ml of CSR.flush @15:28:14.76 CSR.select :flush @15:28:14.87 CS.reconfigure :default @15:28:14.92 CS.seek 5ml @15:28:28.73 delay 5 seconds @15:28:34.10 CSR.select :air @15:28:34.32 CS.reconfigure :fast @15:28:34.37 CS.seek 7.5ml @15:28:41.20 CSV.dial :waste @15:28:42.47 CS.reconfigure :default @15:28:42.52 CS.seek 0ml @15:29:02.51 Purging external Intake @15:29:02.63 CSV.dial :flush @15:29:03.09 CS.reconfigure :fast @15:29:03.14 CS.seek 10ml @15:29:27.79 delay 5 seconds @15:29:32.82 CSV.dial CBV @15:29:32.84
CBV.dial CSV @15:29:33.59 CTV.dial :intake @15:29:34.37 Intake.select :open @15:29:34.48 CS.seek 0ml @15:29:58.16 Intake.select :close @15:29:58.27 CTV.dial :air @15:29:59.03 Flushing upper manifold @15:29:59.15 CSV.dial :flush @15:29:59.88 CS.seek 5ml @15:30:12.85 delay 5 seconds @15:30:17.87 CSV.dial :air @15:30:18.49 CS.seek 7ml @15:30:24.11 CSV.dial CBV @15:30:24.13
CBV.dial CSV @15:30:25.15 CTV.dial :air @15:30:25.23 CS.seek 0ml @15:30:41.95 delay 7 seconds @15:30:48.98 CS.seek 3ml @15:30:57.07 CSV.dial :waste @15:30:57.53 CS.seek 0ml @15:31:05.46 CSV.dial :flush @15:31:05.91 CS.seek 3ml @15:31:14.04 delay 5 seconds @15:31:19.06 CSV.dial :air @15:31:19.55 CS.seek 5ml @15:31:25.16 CSV.dial :air @15:31:25.34 CS.seek 0ml @15:31:37.70 delay 7 seconds @15:31:44.72 CS.seek 3ml @15:31:52.76 CSV.dial :waste @15:31:53.48 CS.seek 0ml @15:32:01.17 Garaging FlushPuck in Collection Clamp @15:32:01.24 Elbow.reconfigure :tight @15:32:01.28 Elbow.seek CC @15:32:01.58 Elbow.reconfigure :loose @15:32:01.68 Forearm.seek CC @15:32:02.64 Hand.close @15:32:03.28 CC.seek :open @15:32:08.34 Forearm.seek :retract @15:32:09.33 Elbow.reconfigure :tight @15:32:09.37 Elbow.seek :garage @15:32:12.27 Elbow.reconfigure :loose @15:32:12.37 Forearm.reconfigure :tight @15:32:12.42 Forearm.seek :garage @15:32:13.37 Hand.open @15:32:14.09 Forearm.reconfigure :loose @15:32:14.13 Forearm.seek :retract @15:32:15.11 da sampling at most 50ml, wcr at most 50ml @15:32:15.15 Loading sh1 puck from tube 2 into Collection Clamp @15:32:15.16 DAfans.email "Started da in tube 2 at 15:32:15UTC12-Jan-24\n{1 => 0,\n 2 => 4,\n 3 => 0,\n 4 => 0,\n 5 => 0,\n 6 => 0,\n 7 => 0}\nCTD data unavailable\nCan@15:27:56, 26% flow, 21.9C, 22% humidity, 15.0psia, 13.749V, 0.321A, 13.877Ah, 4.41W",Subject: "da sampling at most 50ml, wcr at most 50ml" @15:32:15.43
SC.reconfigure :tight @15:32:15.49 SC.seek 2 @15:32:18.99 upsync @15:32:22.24
SC.configure :limp @15:32:22.47 Elbow.reconfigure :tight @15:32:22.52 Elbow.seek 2 @15:32:24.53 Forearm.reconfigure :tight @15:32:24.59 Forearm.seek 2 + 450 counts @15:32:25.67 SE.reconfigure :pick @15:32:25.72 SE.seek :beamBreak @15:32:28.64 SE.configure :default @15:32:28.80 Elbow.seek 2 @15:32:29.00 Forearm.seek 2 + 300 counts @15:32:29.26 Forearm.reconfigure :loose @15:32:29.31 Hand.close @15:32:29.92 SE.seek :down @15:32:30.12
Forearm.seek :retract @15:32:31.09 Elbow.seek CC @15:32:32.56 SE.stop @15:32:32.75
Elbow.reconfigure :loose @15:32:32.86 Forearm.reconfigure :tight @15:32:32.90 Forearm.seek CC @15:32:34.13 CC.seek :closed @15:32:38.64 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.23 @15:32:38.65 Hand.open @15:32:39.34 Forearm.reconfigure :loose @15:32:39.38 Forearm.seek :retract @15:32:40.36 collectSample 50ml w/bubble point of 24psi @15:32:40.37 Priming 7ml @15:32:40.38 Intake.select :close @15:32:40.39 Exhaust.select :close @15:32:40.40 SS.hold @15:32:40.61 Calibrating pressure and temperature sensors @15:32:40.64 CTV.dial :air @15:32:40.65 SSV.dial :spit,avoiding: :exhaust @15:32:40.73 SS.reconfigure :fast @15:32:40.78 SS.seek 5ml @15:32:43.65 SSV.dial CBV @15:32:43.68
CBV.dial SSV @15:32:45.01 delay 3.8 seconds @15:32:48.93 Sensor limits: Intake max 106.7psia; Outlet max 100.9psia @15:32:48.94 SSV.dial :spit @15:32:50.26 SS.seek 0ml @15:32:53.16 SSV.dial CBV @15:32:54.47 CTV.dial :intake @15:32:55.26 SSV.dial CBV @15:32:55.29
CBV.dial SSV @15:32:55.49 Allowing 40s for 7.0ml of remaining 7.0ml @15:32:55.50 Intake.select :open @15:32:57.66 Intake @15.5psia, vacuum from 10.0 to 13.0psi @15:32:57.68 SS.reconfigure :inhaling 2.20ml/s @15:32:57.73 SS.seek 7ml @15:33:04.50 Stroke completed -- waiting for pressure to equalize ... @15:33:04.51 SS.reconfigure :exhale @15:33:04.56 SS.hold @15:33:08.22 Intake.select :close @15:33:08.37 SSV.dial :exhaust @15:33:09.13 Exhaust.select :open @15:33:09.24 SS.seek 0ml @15:33:13.60 Exhaust.select :close @15:33:13.71 Sampling 50.0ml @15:33:13.72 Intake.select :close @15:33:13.73 Exhaust.select :close @15:33:13.74 SS.reconfigure :default @15:33:13.78 SS.hold @15:33:13.99 CTV.dial :intake @15:33:14.23 SSV.dial CBV @15:33:14.26
CBV.dial SSV @15:33:14.99 Allowing 130s for 25.0ml of remaining 50.0ml @15:33:15.00 Intake.select :open @15:33:17.16 Intake @15.8psia, vacuum from 10.0 to 13.0psi @15:33:17.17 SS.reconfigure :inhaling 2.20ml/s @15:33:17.23 SS.seek 25ml @15:33:31.27 Stroke completed -- waiting for pressure to equalize ... @15:33:31.28 SS.reconfigure :exhale @15:33:31.32 SS.hold @15:33:34.84 Intake.select :close @15:33:34.99 SSV.dial :exhaust @15:33:35.75 Exhaust.select :open @15:33:35.87 SS.seek 0ml @15:33:44.74 Exhaust.select :close @15:33:44.85 SSV.dial CBV @15:33:44.87
CBV.dial SSV @15:33:45.63 Allowing 130s for 25.0ml of remaining 25.0ml @15:33:45.64 Intake.select :open @15:33:47.80 Intake @16.0psia, vacuum from 10.0 to 13.0psi @15:33:47.82 SS.reconfigure :inhaling 2.20ml/s @15:33:47.87 SS.seek 25ml @15:34:02.94 Stroke completed -- waiting for pressure to equalize ... @15:34:02.95 SS.reconfigure :exhale @15:34:02.99 SS.hold @15:34:07.35 Intake.select :close @15:34:07.50 SSV.dial :exhaust @15:34:08.25 Exhaust.select :open @15:34:08.36 SS.seek 0ml @15:34:17.27 Exhaust.select :close @15:34:17.38 Sampled 50.0ml @15:34:17.39 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @15:34:17.40 Intake.select :close @15:34:17.47 CSV.dial :waste @15:34:17.58 SSV.dial CBV,avoiding: :exhaust @15:34:17.61
CBV.dial SSV @15:34:17.68 CS.seek 0ml @15:34:17.75 CSV.dial :air @15:34:18.50 CS.seek 10ml @15:34:19.49
CTV.dial :air @15:34:20.24 delay 15 seconds @15:34:35.26 SSV.dial :spit,avoiding: :exhaust @15:34:36.59 CTV.dial CSV,avoiding: :intake @15:34:36.62
SS.seek 0ml @15:34:36.75 SSV.dial CBV,avoiding: :exhaust @15:34:43.20 SSV.dial between CBV and plug @15:34:43.68 CBV.dial between SSV and waste2 @15:34:44.07 CTV.dial CSV,avoiding: :air @15:34:44.10
CSV.dial CTV @15:34:44.62 CS.reconfigure :pressurize @15:34:44.67 CS.seek 0ml @15:35:03.23 BubblePoint: Intake @24.3psia, Outlet @0.6psia @15:35:03.24 CBV.dial SSV @15:35:03.57 SS.reconfigure :default @15:35:03.62 SS.seek 10ml @15:35:09.42 delay 15 seconds @15:35:24.55 Evacuated: Intake @23.9psia, Outlet @24.1psia @15:35:24.56 SSV.dial :spit,avoiding: :exhaust @15:35:25.58 SS.reconfigure :exhale @15:35:25.63 SS.seek 0ml @15:35:31.01 SS.reconfigure :fast @15:35:31.05 SS.seek 5ml @15:35:34.02 SSV.dial CBV,avoiding: :exhaust @15:35:34.05
CBV.dial SSV @15:35:35.38 CTV.dial :air,avoiding: :intake @15:35:37.11 CSV.dial CTV @15:35:37.16
SS.seek 10ml @15:35:38.34 CSV.dial :air @15:35:38.77 CS.reconfigure :fast @15:35:38.83 CS.seek 0ml @15:35:39.77
SSV.dial :spit,avoiding: :exhaust @15:35:41.08 SS.reconfigure :exhale @15:35:41.13 SS.seek 0ml @15:35:46.34 CS.stop @15:35:46.43
SS.reconfigure :fast @15:35:46.48 SS.seek 5ml @15:35:49.33 SSV.dial CBV,avoiding: :exhaust @15:35:49.36
CBV.dial SSV @15:35:51.73 SSV.dial :spit,avoiding: :exhaust @15:35:53.05 SS.reconfigure :default @15:35:53.10 SS.seek 0ml @15:35:56.45 SS.stop @15:35:56.49 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @15:35:56.50 Intake.select :close @15:35:56.57 CSV.dial :waste @15:35:56.69 SSV.dial CBV,avoiding: :exhaust @15:35:56.72
CBV.dial SSV @15:35:57.34 CS.seek 0ml @15:35:57.41 CSV.dial :air @15:35:58.06
CTV.dial :air @15:35:58.24 delay 15 seconds @15:35:58.28 CS.seek 10ml @15:36:13.26
SSV.dial :spit,avoiding: :exhaust @15:36:14.58 CTV.dial CSV,avoiding: :intake @15:36:14.62
SS.reconfigure :exhale @15:36:14.72 SS.seek 0ml @15:36:15.13 SSV.dial CBV,avoiding: :exhaust @15:36:22.63 SSV.dial between CBV and plug @15:36:23.10 CBV.dial between SSV and waste2 @15:36:23.48 CSV.dial between air and CTV @15:36:23.81 CS.seek 8.265ml @15:36:28.60 CTV.dial CSV,avoiding: :air @15:36:28.63
CSV.dial CTV @15:36:29.01 CS.reconfigure :pressurize @15:36:29.06 CS.seek 0ml @15:36:47.07 BubblePoint: Intake @24.0psia, Outlet @1.4psia @15:36:47.08 CBV.dial SSV @15:36:47.41 SS.reconfigure :default @15:36:47.46 SS.seek 10ml @15:36:53.12 delay 15 seconds @15:37:08.25 Evacuated: Intake @23.8psia, Outlet @15.1psia @15:37:08.26 SSV.dial :spit,avoiding: :exhaust @15:37:09.30 SS.reconfigure :exhale @15:37:09.35 SS.seek 0ml @15:37:14.36 SS.reconfigure :fast @15:37:14.41 SS.seek 5ml @15:37:17.72 SSV.dial CBV,avoiding: :exhaust @15:37:17.75
CBV.dial SSV @15:37:19.10 CTV.dial :air,avoiding: :intake @15:37:20.40 CSV.dial CTV @15:37:20.45
SS.seek 10ml @15:37:21.55 CSV.dial :air @15:37:22.01 CS.reconfigure :fast @15:37:22.06 CS.seek 0ml @15:37:22.92
SSV.dial :spit,avoiding: :exhaust @15:37:24.26 SS.reconfigure :exhale @15:37:24.31 SS.seek 0ml @15:37:26.41 CS.stop @15:37:29.68
SS.reconfigure :fast @15:37:29.73 SS.seek 5ml @15:37:32.55 SSV.dial CBV,avoiding: :exhaust @15:37:32.58
CBV.dial SSV @15:37:34.91 SSV.dial :spit,avoiding: :exhaust @15:37:36.22 SS.reconfigure :default @15:37:36.27 SS.seek 0ml @15:37:39.64 SS.stop @15:37:39.68 Lysing: Adding 2.0ml AqMeOH @15:37:39.69 CBV.dial between CSV and waste1,avoiding: :DBV @15:37:41.17 CTV.dial CSV @15:37:42.47 CSV.dial CSR @15:37:43.37 Pulling 1ml of CSR.AqMeOH @15:37:43.38 CSR.select :AqMeOH @15:37:43.50 CS.reconfigure :default @15:37:43.55 CS.seek 1ml @15:37:47.10 delay 5 seconds @15:37:52.11 CSR.select :air @15:37:52.28 CS.seek 6ml @15:38:05.97 delay 2 seconds @15:38:07.99 CSV.dial :waste2 @15:38:08.45 CS.reconfigure :fast @15:38:08.49 CS.seek 0ml @15:38:22.88 CSV.dial CSR @15:38:23.33 Pulling 2ml of CSR.AqMeOH @15:38:23.34 CSR.select :AqMeOH @15:38:23.45 CS.reconfigure :default @15:38:23.50 CS.seek 2ml @15:38:29.47 delay 5 seconds @15:38:34.49 CSR.select :air @15:38:34.65 CS.seek 5ml @15:38:43.13 delay 2 seconds @15:38:45.14 CSV.dial CTV @15:38:45.58 CS.seek 1ml @15:38:56.76 Before lysing Collection Puck Heater at 21.8C/25.2psia @15:38:56.78 Heating Puck.from 2, :sh1 in Collection Clamp to 70C for 10:00 @15:38:56.80 CH.seek 70C,8:00 @15:40:47.30 CH.hold 10:00,70C @15:40:47.49 Begun lysing Collection Puck Heater at 68C/31.2psia @15:50:47.51 Ended lysing Collection Puck Heater at 70C/29.8psia @15:50:47.52 CS.seek 0ml @15:50:51.12 CTV.dial between spare and air,avoiding: :intake @15:50:52.13 CSV.dial :air @15:50:52.58 CS.seek 5ml @15:51:06.42 CSV.dial CTV @15:51:06.88 delay 2 seconds @15:51:08.90 CSV.dial :air @15:51:09.35 delay 2 seconds @15:51:11.36 CSV.dial CTV @15:51:11.83 delay 2 seconds @15:51:13.84 CSV.dial :air @15:51:14.30 delay 2 seconds @15:51:16.32 CS.seek 0ml @15:51:30.01 Puck @58.4psia after final lysis pressurization @15:51:30.02 Recovering Lysate into Collection syringe @15:51:30.03 CSV.dial CBV @15:51:31.02 CBV.dial CSV @15:51:31.84 CS.seek 5ml @15:51:45.39 delay 5 seconds @15:51:50.41 CTV.dial :air @15:51:50.93 CS.seek 7ml @15:51:56.80 delay 5 seconds @15:52:01.82 CSV.dial :air2 @15:52:02.28 CS.seek 10ml @15:52:10.93 CSV.dial between air and CTV,avoiding: CBV @15:52:12.07 Lysing Complete @15:52:12.14 Storing sh1 puck in Collection Clamp to tube 1 @15:52:12.31 SC.reconfigure :tight @15:52:12.36 SC.seek 1 @15:52:15.95 SC.configure :limp @15:52:16.10 SE.reconfigure :place @15:52:16.18
Elbow.reconfigure :tight @15:52:16.22 SE.seek :beamBreak @15:52:16.25
Elbow.seek CC @15:52:16.57 Elbow.reconfigure :loose @15:52:16.67 Forearm.seek CC @15:52:17.71 Hand.close @15:52:18.39 CC.seek :open @15:52:19.08 SE.configure :default @15:52:19.14 SE.seek :place @15:52:23.16
Forearm.seek :retract @15:52:24.11 Elbow.reconfigure :tight @15:52:24.15 Elbow.seek 1 @15:52:25.87 Elbow.reconfigure :limp @15:52:25.92 Forearm.seek 1 @15:52:26.96 Elbow.hold @15:52:27.03 Elbow.reconfigure :tight @15:52:27.08 Hand.open @15:52:27.75 Forearm.force -20 @15:52:27.80 Forearm.seek 1 + 310 counts @15:52:28.10 Forearm.reconfigure :tight @15:52:28.15 Forearm.seek 1 + 450 counts @15:52:28.72 SE.seek :down @15:52:29.85
Forearm.reconfigure :loose @15:52:29.91 Forearm.seek :retract @15:52:31.44 SE.stop @15:52:31.48
Tranferring DA @15:52:31.49 Beginning samplePassC @15:52:31.51 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @15:52:31.69 Elbow.seek FlushPuck::Garage @15:52:33.51 Elbow.reconfigure :loose @15:52:33.61 Forearm.seek :garage @15:52:34.57 Hand.close @15:52:35.25 Forearm.seek :retract @15:52:36.26 Elbow.reconfigure :tight @15:52:36.31 Elbow.seek CC @15:52:39.24 Elbow.reconfigure :loose @15:52:39.34 Forearm.reconfigure :tight @15:52:39.39 Forearm.seek CC @15:52:40.66 CC.seek :closed @15:52:45.22 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.34 @15:52:45.30 Hand.open @15:52:45.95 Forearm.reconfigure :loose @15:52:46.00 Forearm.seek :retract @15:52:46.99 PSV.dial PSR @15:52:48.35 Pulling 1.5ml of PSR.flush @15:52:48.36 PSR.select :flush @15:52:48.48 PS.seek 1.5ml @15:53:04.56 delay 5 seconds @15:53:09.58 PSV.dial :waste1 @15:53:10.11 PS.seek 0ml @15:53:26.31 PSV.dial PSR @15:53:26.78 PSR.select :air @15:53:26.90 PS.seek 2.5ml @15:53:53.29 PRV.dial CBV,avoiding: :ab2 @15:53:53.31
CBV.dial PRV @15:53:55.03 PSV.dial :mixloop @15:53:56.05 CTV.dial :air,avoiding: :intake @15:53:56.28 PTV.dial :PRVmixing @15:53:57.58 PS.seek 0ml @15:54:23.93 PSV.dial :air @15:54:24.52 PS.seek 2.5ml @15:54:50.81 PSV.dial :mixloop @15:54:51.27 PS.seek 0ml @15:55:17.62 PSV.dial :air @15:55:18.10 PS.seek 2.5ml @15:55:44.46 PSV.dial :mixloop @15:55:44.94 PS.seek 0ml @15:56:11.28 Garaging FlushPuck in Collection Clamp @15:56:11.35 Elbow.reconfigure :tight @15:56:11.39 Elbow.seek CC @15:56:11.63 Elbow.reconfigure :loose @15:56:11.73 Forearm.seek CC @15:56:12.77 Hand.close @15:56:13.39 CC.seek :open @15:56:18.04 Forearm.seek :retract @15:56:19.01 Elbow.reconfigure :tight @15:56:19.06 Elbow.seek :garage @15:56:22.02 Elbow.reconfigure :loose @15:56:22.12 Forearm.reconfigure :tight @15:56:22.16 Forearm.seek :garage @15:56:23.19 Hand.open @15:56:23.82 Forearm.reconfigure :loose @15:56:23.86 Forearm.seek :retract @15:56:24.83 PSV.dial :mixloop @15:56:24.92 PTV.dial :PRVmixing @15:56:25.00 PRV.dial CBV @15:56:25.03
CBV.dial PRV @15:56:25.25 CTV.dial :air,avoiding: :intake @15:56:25.71 Loading lyfil puck from tube 2 into Collection Clamp @15:56:25.94 SC.reconfigure :tight @15:56:25.99 SC.seek 2 @15:56:29.59 SC.configure :limp @15:56:29.81 Elbow.reconfigure :tight @15:56:29.86 Elbow.seek 2 @15:56:31.76 Forearm.reconfigure :tight @15:56:31.81 Forearm.seek 2 + 450 counts @15:56:32.81 SE.reconfigure :pick @15:56:32.87 SE.seek :beamBreak @15:56:35.86 SE.configure :default @15:56:36.02 Elbow.seek 2 @15:56:36.21 Forearm.seek 2 + 300 counts @15:56:36.48 Forearm.reconfigure :loose @15:56:36.52 Hand.close @15:56:37.15 SE.seek :down @15:56:37.35
Forearm.seek :retract @15:56:38.31 Elbow.seek CC @15:56:39.84 SE.stop @15:56:39.97
Elbow.reconfigure :loose @15:56:40.07 Forearm.reconfigure :tight @15:56:40.12 Forearm.seek CC @15:56:41.43 CC.seek :closed @15:56:45.96 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.11 @15:56:45.97 Hand.open @15:56:46.69 Forearm.reconfigure :loose @15:56:46.74 Forearm.seek :retract @15:56:47.74 CTV.dial CSV,avoiding: :intake @15:56:49.05 CSV.dial CTV @15:56:49.37 PS.seek 2.5ml @15:57:15.88 CS.seek 4.5ml @15:57:30.80 delay 8 seconds @15:57:38.82 PSV.dial :air @15:57:39.40 delay 2 seconds @15:57:41.42 PSV.dial :waste2 @15:57:42.17 PS.seek 0ml @15:58:08.55 CBV.dial between PRV and waste1 @15:58:08.89 CSV.dial :air @15:58:09.35 CS.seek 10ml @15:58:24.29 CSV.dial CTV @15:58:24.75 CS.seek 0ml @15:58:51.14 CBV.dial :waste1 @15:58:51.44 delay 5 seconds @15:58:56.46 CTV.dial :air,avoiding: :intake @15:58:57.84 Storing lyfil puck in Collection Clamp to tube 1 @15:58:58.01 SC.reconfigure :tight @15:58:58.06 SC.seek 1 @15:59:01.61 SC.configure :limp @15:59:01.83 Elbow.reconfigure :tight @15:59:01.87 Elbow.seek 1 @15:59:03.66 Forearm.reconfigure :tight @15:59:03.70 Forearm.seek 1 + 450 counts @15:59:04.72 SE.reconfigure :pick @15:59:04.78 SE.seek :beamBreak @15:59:08.24 SE.configure :default @15:59:08.40 Elbow.reconfigure :limp @15:59:08.46 Forearm.reconfigure :loose @15:59:08.50 Forearm.seek 1 @15:59:08.75 Elbow.hold @15:59:08.82 Elbow.reconfigure :tight @15:59:08.92 SE.seek 0.17 Pucks below Top Plate @15:59:09.38 Forearm.force -20 @15:59:09.42 Forearm.seek 1 + 320 counts @15:59:09.70 Forearm.reconfigure :tight @15:59:09.75 Forearm.seek 1 + 450 counts @15:59:09.95 SE.seek 1.17 Pucks below Top Plate @15:59:10.46 Forearm.reconfigure :loose @15:59:10.51 Forearm.seek :retract @15:59:11.45 Elbow.seek CC @15:59:13.12 Elbow.reconfigure :loose @15:59:13.22 Forearm.seek CC @15:59:14.27 Hand.close @15:59:14.88 CC.seek :open @15:59:19.65 Forearm.seek :retract @15:59:20.66 Elbow.reconfigure :tight @15:59:20.71 Elbow.seek 1 @15:59:22.48 Elbow.reconfigure :limp @15:59:22.53 Forearm.seek 1 @15:59:23.54 Elbow.hold @15:59:23.60 Elbow.reconfigure :tight @15:59:23.65 Hand.open @15:59:24.82 Forearm.force -20 @15:59:24.86 Forearm.seek 1 + 319 counts @15:59:25.17 Forearm.reconfigure :tight @15:59:25.22 Forearm.seek 1 + 450 counts @15:59:25.46 SE.seek :down @15:59:26.60
Forearm.reconfigure :loose @15:59:26.65 Forearm.seek :retract @15:59:28.07 SE.stop @15:59:28.11
PSV.dial :air @15:59:28.86 PS.seek 2.5ml @15:59:55.21 delay 1 second @15:59:56.22 PSV.dial :mixloop @15:59:56.79 delay 1 second @15:59:57.80 PSV.dial :air @15:59:58.26 delay 1 second @15:59:59.27 PSV.dial :mixloop @15:59:59.74 delay 1 second @16:00:00.76 PSV.dial :air @16:00:01.34 delay 1 second @16:00:02.36 PS.seek 0ml @16:00:28.71 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @16:00:28.89 Elbow.seek FlushPuck::Garage @16:00:30.70 Elbow.reconfigure :loose @16:00:30.80 Forearm.seek :garage @16:00:31.75 Hand.close @16:00:32.49 Forearm.seek :retract @16:00:33.46 Elbow.reconfigure :tight @16:00:33.51 Elbow.seek PC @16:00:34.78 Elbow.reconfigure :loose @16:00:34.88 Forearm.reconfigure :tight @16:00:34.93 Forearm.seek PC @16:00:36.57 PC.seek :closed @16:00:41.22 Processing Clamp onSpring/offSpring for 16/26 samples, on/off torque 1.58 @16:00:41.30 Hand.open @16:00:41.94 Forearm.reconfigure :loose @16:00:41.99 Forearm.seek :retract @16:00:42.94 PRV.dial :air @16:00:43.40 PSV.dial :mixloop @16:00:43.91 PS.seek 0.3ml @16:00:47.86 PSV.dial PSR @16:00:48.90 Pulling 0.4ml of PSR.air @16:00:48.91 PSR.select :air @16:00:48.93 PS.seek 0.7ml @16:00:53.81 delay 3 seconds @16:00:56.83 PSR.select :air @16:00:56.85 PS.seek 2.5ml @16:01:16.05 delay 3 seconds @16:01:19.07 PSV.dial :mixloop @16:01:20.10 PTV.dial :mixing @16:01:20.83 PBV.dial :waste2 @16:01:21.78 PS.seek 0ml @16:01:48.12 Garaging FlushPuck in Processing Clamp @16:01:48.19 Elbow.reconfigure :tight @16:01:48.23 Elbow.seek PC @16:01:48.36 Elbow.reconfigure :loose @16:01:48.46 Forearm.seek PC @16:01:49.44 Hand.close @16:01:50.01 PC.seek :open @16:01:54.73 Forearm.seek :retract @16:01:55.68 Elbow.reconfigure :tight @16:01:55.72 Elbow.seek :garage @16:01:57.09 Elbow.reconfigure :loose @16:01:57.19 Forearm.reconfigure :tight @16:01:57.23 Forearm.seek :garage @16:01:58.63 Hand.open @16:01:59.32 Forearm.reconfigure :loose @16:01:59.37 Forearm.seek :retract @16:02:00.38 Loading da puck from tube 2 into Processing Clamp @16:02:00.62 SC.reconfigure :tight @16:02:00.67 SC.seek 2 @16:02:04.22 SC.configure :limp @16:02:04.43 Elbow.reconfigure :tight @16:02:04.47 Elbow.seek 2 @16:02:06.32 Forearm.reconfigure :tight @16:02:06.37 Forearm.seek 2 + 450 counts @16:02:07.39 SE.reconfigure :pick @16:02:07.45 SE.seek :beamBreak @16:02:10.50 SE.configure :default @16:02:10.65 Elbow.seek 2 @16:02:10.84 Forearm.seek 2 + 300 counts @16:02:11.05 Forearm.reconfigure :loose @16:02:11.09 Hand.close @16:02:11.77 SE.seek :down @16:02:11.97
Forearm.seek :retract @16:02:12.91 Elbow.seek PC @16:02:14.51 SE.stop @16:02:15.45
Elbow.reconfigure :loose @16:02:15.55 Forearm.reconfigure :tight @16:02:15.59 Forearm.seek PC @16:02:16.81 PC.seek :closed @16:02:21.43 Processing Clamp onSpring/offSpring for 15/25 samples, on/off torque 1.61 @16:02:21.44 Hand.open @16:02:22.06 Forearm.reconfigure :loose @16:02:22.11 Forearm.seek :retract @16:02:23.11 Heating Puck.from 2, :da in Processing Clamp to 28C for 2:00:00 @16:02:23.12 PH.seek 28C,5:00 @16:02:53.79 PH.hold 2:00:00,28C @16:02:53.83 delay 1:30 @16:04:23.86 PSR.select :air @16:04:23.88 PSV.dial PSR @16:04:24.91 Pulling 0.5ml of PSR.wash @16:04:24.92 PSR.select :wash @16:04:25.04 PS.seek 0.5ml @16:04:30.95 delay 5 seconds @16:04:35.97 PSR.select :air @16:04:36.09 PS.seek 2ml @16:04:52.33 PTV.dial :puck @16:04:53.06 PSV.dial PTV @16:04:53.81 PBV.dial :waste1 @16:04:55.62 PS.seek 1.5ml @16:05:01.58 delay 3 seconds @16:05:04.60 PBV.dial between PSV and waste1 @16:05:04.94 PS.seek 0ml @16:05:21.08 delay 5:00 @16:10:21.10 recoverPSRreagent08 @16:10:21.11 PTV.dial :puck @16:10:21.18 PBV.dial :waste1 @16:10:21.56 delay 30 seconds @16:10:51.58 PBV.dial between PSV and waste1 @16:10:51.95 PSR.select :air @16:10:51.96 PSV.dial PSR @16:10:52.72 PS.reconfigure :fast @16:10:52.77 PS.seek 2.5ml @16:11:16.32 PSV.dial PTV @16:11:17.07 delay 5 seconds @16:11:22.09 PSV.dial PSR @16:11:22.84 delay 5 seconds @16:11:27.86 PSV.dial PTV @16:11:28.62 delay 5 seconds @16:11:33.64 PSV.dial :air @16:11:34.40 PS.seek 0ml @16:11:57.39 PTV.dial :PRVmixing,via: PRV @16:11:58.70 PSV.dial :mixloop @16:11:59.16 PRV.dial :ab1 @16:11:59.89 PS.reconfigure :slow @16:11:59.94 PS.seek 0.15ml @16:12:03.48 delay 2 seconds @16:12:05.50 CBV.dial PRV @16:12:05.52
PRV.dial CBV @16:12:06.09 PS.seek 0.34ml @16:12:10.41 delay 2 seconds @16:12:12.43 CBV.dial between PRV and PSV @16:12:12.75 PRV.dial :air @16:12:13.20 PS.seek 0.9ml @16:12:25.16 PRV.dial :waste2 @16:12:25.90 PS.seek 0.5ml @16:12:34.60 addPMLReagent08 @16:12:34.61 PSV.dial :air @16:12:35.07 PS.reconfigure :default @16:12:35.12 PS.seek 2ml @16:12:51.32 PSV.dial :PML @16:12:51.79 PTV.dial :mixing @16:12:52.52 PBV.dial :waste1 @16:12:52.96 PS.seek 1.85ml @16:12:55.32 PBV.dial between PSV and waste1 @16:12:55.68 PS.seek 0ml @16:13:15.41 delay 2 seconds @16:13:17.43 Beginning samplePassC @16:13:17.44 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @16:13:18.06 Elbow.reconfigure :tight @16:13:18.11 Elbow.seek FlushPuck::Garage @16:13:19.49 Elbow.reconfigure :loose @16:13:19.59 Forearm.seek :garage @16:13:20.61 Hand.close @16:13:21.36 Forearm.seek :retract @16:13:22.37 Elbow.reconfigure :tight @16:13:22.42 Elbow.seek CC @16:13:25.28 Elbow.reconfigure :loose @16:13:25.38 Forearm.reconfigure :tight @16:13:25.43 Forearm.seek CC @16:13:26.71 CC.seek :closed @16:13:31.23 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.34 @16:13:31.29 Hand.open @16:13:31.93 Forearm.reconfigure :loose @16:13:31.97 Forearm.seek :retract @16:13:32.93 PSV.dial PSR @16:13:34.08 Pulling 1.5ml of PSR.flush @16:13:34.09 PSR.select :flush @16:13:34.21 PS.seek 1.5ml @16:13:50.44 delay 5 seconds @16:13:55.46 PSV.dial :waste1 @16:13:55.93 PS.seek 0ml @16:14:12.13 PSV.dial PSR @16:14:12.58 PSR.select :air @16:14:12.70 PS.seek 2.5ml @16:14:39.51 PRV.dial CBV,avoiding: :ab2 @16:14:39.54
CBV.dial PRV @16:14:41.13 PSV.dial :mixloop @16:14:42.27 CTV.dial :air,avoiding: :intake @16:14:42.36 PTV.dial :PRVmixing @16:14:43.10 PS.seek 0ml @16:15:09.47 PSV.dial :air @16:15:09.95 PS.seek 2.5ml @16:15:36.25 PSV.dial :mixloop @16:15:36.73 PS.seek 0ml @16:16:03.00 PSV.dial :air @16:16:03.48 PS.seek 2.5ml @16:16:29.84 PSV.dial :mixloop @16:16:30.32 PS.seek 0ml @16:16:56.63 Garaging FlushPuck in Collection Clamp @16:16:56.70 Elbow.reconfigure :tight @16:16:56.75 Elbow.seek CC @16:16:57.03 Elbow.reconfigure :loose @16:16:57.13 Forearm.seek CC @16:16:58.18 Hand.close @16:16:58.75 CC.seek :open @16:17:03.36 Forearm.seek :retract @16:17:04.38 Elbow.reconfigure :tight @16:17:04.43 Elbow.seek :garage @16:17:07.39 Elbow.reconfigure :loose @16:17:07.49 Forearm.reconfigure :tight @16:17:07.54 Forearm.seek :garage @16:17:08.57 Hand.open @16:17:09.30 Forearm.reconfigure :loose @16:17:09.35 Forearm.seek :retract @16:17:10.34 delayUntil "16:21:17.42" @16:17:10.36 Whole Cell Archive sampling 50ml @16:17:10.38 Loading wcr puck from tube 2 into Collection Clamp @16:17:11.13 SC.reconfigure :tight @16:17:11.18 SC.seek 2 @16:17:11.48 SC.configure :limp @16:17:11.71 Elbow.reconfigure :tight @16:17:11.77 Elbow.seek 2 @16:17:13.72 Forearm.reconfigure :tight @16:17:13.77 Forearm.seek 2 + 450 counts @16:17:14.79 SE.reconfigure :pick @16:17:14.86 SE.seek :beamBreak @16:17:17.88 SE.configure :default @16:17:18.05 Elbow.seek 2 @16:17:18.24 Forearm.seek 2 + 300 counts @16:17:18.54 Forearm.reconfigure :loose @16:17:18.59 Hand.close @16:17:19.25 Puck::Warning in WCR -- Loaded Last remaining Puck (from Tube 2) @16:17:19.27 SE.seek :down @16:17:19.49 Forearm.seek :retract @16:17:20.41 Elbow.seek CC @16:17:21.98 Elbow.reconfigure :loose @16:17:22.05 SE.stop @16:17:22.16 Forearm.reconfigure :tight @16:17:22.21 Forearm.seek CC @16:17:23.50 CC.seek :closed @16:17:28.03 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.22 @16:17:28.05 Hand.open @16:17:28.69 Forearm.reconfigure :loose @16:17:28.74 Forearm.seek :retract @16:17:29.77 collectSample 50ml w/bubble point of 35psi @16:17:29.79 Priming 7ml @16:17:29.80 Intake.select :close @16:17:29.81 Exhaust.select :close @16:17:29.82 SS.hold @16:17:30.03 Calibrating pressure sensors @16:17:30.04 CTV.dial :air @16:17:30.12 SSV.dial :spit,avoiding: :exhaust @16:17:30.21 SS.reconfigure :fast @16:17:30.26 SS.seek 5ml @16:17:32.99 SSV.dial CBV @16:17:33.02 CBV.dial SSV @16:17:34.34 delay 3.8 seconds @16:17:38.28 Sensor limits: Intake max 108.5psia; Outlet max 100.9psia @16:17:38.29 SSV.dial :spit @16:17:39.62 SS.seek 0ml @16:17:42.45 SSV.dial CBV @16:17:43.75 CTV.dial :intake @16:17:44.50 SSV.dial CBV @16:17:44.54 CBV.dial SSV @16:17:44.68 Allowing 40s for 7.0ml of remaining 7.0ml @16:17:44.70 Intake.select :open @16:17:46.87 Intake @15.7psia, vacuum from 10.0 to 13.0psi @16:17:46.89 SS.reconfigure :inhaling 2.20ml/s @16:17:46.93 SS.seek 7ml @16:17:53.43 Stroke completed -- waiting for pressure to equalize ... @16:17:53.44 SS.reconfigure :exhale @16:17:53.49 SS.hold @16:17:59.31 Intake.select :close @16:17:59.47 SSV.dial :exhaust @16:18:00.23 Exhaust.select :open @16:18:00.35 SS.seek 0ml @16:18:04.70 Exhaust.select :close @16:18:04.81 Sampling 50.0ml @16:18:04.83 Intake.select :close @16:18:04.84 Exhaust.select :close @16:18:04.85 SS.reconfigure :default @16:18:04.90 SS.hold @16:18:05.12 CTV.dial :intake @16:18:05.29 SSV.dial CBV @16:18:05.32 CBV.dial SSV @16:18:06.08 Allowing 130s for 25.0ml of remaining 50.0ml @16:18:06.09 Intake.select :open @16:18:08.25 Intake @16.2psia, vacuum from 10.0 to 13.0psi @16:18:08.27 SS.reconfigure :inhaling 2.20ml/s @16:18:08.32 SS.seek 25ml @16:18:19.77 Vacuum too high -- waiting up to 40 seconds @16:18:19.78 SS.reconfigure :exhale @16:18:19.84 SS.hold @16:18:20.52 Intake @12.9psia, vacuum from 7.4 to 10.4psi @16:18:20.54 SS.reconfigure :inhaling 1.76ml/s @16:18:20.58 SS.seek 25ml @16:18:24.32 Stroke completed -- waiting for pressure to equalize ... @16:18:24.33 SS.reconfigure :exhale @16:18:24.38 SS.hold @16:18:28.37 Intake.select :close @16:18:28.53 SSV.dial :exhaust @16:18:29.29 Exhaust.select :open @16:18:29.41 SS.seek 0ml @16:18:38.31 Exhaust.select :close @16:18:38.42 SSV.dial CBV @16:18:38.45 CBV.dial SSV @16:18:39.22 Allowing 130s for 25.0ml of remaining 25.0ml @16:18:39.23 Intake.select :open @16:18:41.39 Intake @16.3psia, vacuum from 10.0 to 13.0psi @16:18:41.41 SS.reconfigure :inhaling 1.76ml/s @16:18:41.46 SS.seek 25ml @16:18:59.07 Stroke completed -- waiting for pressure to equalize ... @16:18:59.08 SS.reconfigure :exhale @16:18:59.13 SS.hold @16:19:03.29 Intake.select :close @16:19:03.45 SSV.dial :exhaust @16:19:04.21 Exhaust.select :open @16:19:04.32 SS.seek 0ml @16:19:13.17 Exhaust.select :close @16:19:13.27 Sampled 50.0ml @16:19:13.73 Drying puck before fixing for 5:00 @16:19:13.74 CBV.dial :waste2b @16:19:14.49 delay 1:00 @16:20:14.52 CSV.dial :air @16:20:14.98 CS.reconfigure :fast @16:20:15.03 CS.seek 10ml @16:20:39.40 Pumping wcr puck from tube 2 up to 35psi... @16:20:39.42 CBV.dial SSV @16:20:40.67 CBV.dial between CSV and waste2b @16:20:41.61 CSV.dial between air and CTV @16:20:41.94 CS.seek 7.302ml @16:20:48.81 CTV.dial CSV,avoiding: :air @16:20:48.84 CSV.dial CTV @16:20:49.64 CS.reconfigure :pressurize @16:20:49.69 CS.seek 0ml @16:21:08.93 CSV.dial :air @16:21:09.39 CS.reconfigure :fast @16:21:09.44 CS.seek 10ml @16:21:17.43 recoverPRVreagent08 @16:21:17.44 PTV.dial :mixing @16:21:18.18 PBV.dial :waste1 @16:21:18.56 delay 30 seconds @16:21:33.89 CSV.dial between air and CTV @16:21:34.22 CS.seek 5.551ml @16:21:45.12 CTV.dial CSV,avoiding: :air @16:21:45.15 CSV.dial CTV @16:21:45.54 CS.reconfigure :pressurize @16:21:45.59 CS.seek 0ml @16:21:48.58 PBV.dial between PSV and waste1 @16:21:48.95 PSV.dial :air @16:21:49.53 PS.reconfigure :fast @16:21:49.58 PS.seek 2.5ml @16:22:00.33 CSV.dial :air @16:22:00.79 CS.reconfigure :fast @16:22:00.85 CS.seek 10ml @16:22:13.31 PSV.dial :mixloop @16:22:13.76 delay 2 seconds @16:22:15.78 PSV.dial :air @16:22:16.25 delay 2 seconds @16:22:18.26 PSV.dial :mixloop @16:22:19.11 delay 2 seconds @16:22:21.19 PSV.dial :air @16:22:21.66 PS.seek 0ml @16:22:25.42 CSV.dial between air and CTV @16:22:25.75 CS.seek 5.682ml @16:22:36.34 CTV.dial CSV,avoiding: :air @16:22:36.39 CSV.dial CTV @16:22:36.74 CS.reconfigure :pressurize @16:22:36.80 CS.seek 0ml @16:22:44.94 washCycleDA #1 of 2 @16:22:44.95 PSR.select :air @16:22:44.96 PSV.dial PSR @16:22:46.28 Pulling 0.5ml of PSR.wash @16:22:46.29 PSR.select :wash @16:22:46.40 PS.reconfigure :default @16:22:46.45 PS.seek 0.5ml @16:22:51.83 CSV.dial :air @16:22:52.29 CS.reconfigure :fast @16:22:52.34 CS.seek 10ml @16:22:52.43 delay 5 seconds @16:22:57.45 PSR.select :air @16:22:57.56 PS.seek 2ml @16:23:13.65 PTV.dial :puck @16:23:14.38 PSV.dial PTV @16:23:15.12 PBV.dial :waste1 @16:23:15.55 PS.seek 1.5ml @16:23:16.92 CSV.dial between air and CTV @16:23:17.25 CS.seek 5.733ml @16:23:21.46 delay 3 seconds @16:23:24.48 PBV.dial between PSV and waste1 @16:23:24.86 PS.seek 0ml @16:23:27.93 CTV.dial CSV,avoiding: :air @16:23:27.96 CSV.dial CTV @16:23:28.32 CS.reconfigure :pressurize @16:23:28.37 CS.seek 0ml @16:23:40.96 delay 2:00 @16:23:43.55 CSV.dial :air @16:23:44.00 CS.reconfigure :default @16:23:44.06 CS.hold @16:23:44.17 ShallowSampler::Error in MAIN -- Puck.from 2, :wcr reached only 23.9 of 35psi after pumping in 40ml of gas @16:23:44.19 Waiting to Resume from Checkpoint "Pumping wcr puck from tube 2 up to 35psi..." @16:23:44.21 BadNews.email "Puck.from 2, :wcr reached only 23.9 of 35psi after pumping in 40ml of gas",Subject: "ShallowSampler::Error in MAIN" @16:23:48.85 upsync @16:25:40.99 recoverPSRreagent08 @16:25:41.00 PTV.dial :puck @16:25:41.09 PBV.dial :waste1 @16:25:41.48 delay 30 seconds @16:26:11.51 PBV.dial between PSV and waste1 @16:26:11.87 PSR.select :air @16:26:11.89 PSV.dial PSR @16:26:12.65 PS.reconfigure :fast @16:26:12.71 PS.seek 2.5ml @16:26:36.40 PSV.dial PTV @16:26:37.15 delay 5 seconds @16:26:42.17 PSV.dial PSR @16:26:42.91 delay 5 seconds @16:26:47.93 PSV.dial PTV @16:26:48.68 delay 5 seconds @16:26:53.70 PSV.dial :air @16:26:54.46 PS.seek 0ml @16:27:17.74 washCycleDA #2 of 2 @16:27:17.75 PSR.select :air @16:27:17.77 PSV.dial PSR @16:27:19.08 Pulling 0.5ml of PSR.wash @16:27:19.09 PSR.select :wash @16:27:19.21 PS.reconfigure :default @16:27:19.26 PS.seek 0.5ml @16:27:25.27 delay 5 seconds @16:27:30.29 PSR.select :air @16:27:30.41 PS.seek 2ml @16:27:46.52 PTV.dial :puck @16:27:46.60 PSV.dial PTV @16:27:47.33 PBV.dial :waste1 @16:27:47.77 PS.seek 1.5ml @16:27:53.68 delay 3 seconds @16:27:56.70 PBV.dial between PSV and waste1 @16:27:57.07 PS.seek 0ml @16:28:13.24 delay 2:00 @16:30:13.26 recoverPSRreagent08 @16:30:13.27 PTV.dial :puck @16:30:13.34 PBV.dial :waste1 @16:30:13.72 delay 30 seconds @16:30:43.75 PBV.dial between PSV and waste1 @16:30:44.11 PSR.select :air @16:30:44.13 PSV.dial PSR @16:30:44.88 PS.reconfigure :fast @16:30:44.93 PS.seek 2.5ml @16:31:08.67 PSV.dial PTV @16:31:09.42 delay 5 seconds @16:31:14.45 PSV.dial PSR @16:31:15.19 delay 5 seconds @16:31:20.21 PSV.dial PTV @16:31:21.08 delay 5 seconds @16:31:26.10 PSV.dial :air @16:31:26.87 PS.seek 0ml @16:31:49.95 getPMLReagent08 @16:31:49.97 PRV.dial :air @16:31:50.41 delay 5 seconds @16:31:55.86 PTV.dial :PRVmixing @16:31:57.19 delay 5 seconds @16:32:02.21 PRV.dial :ab2 @16:32:02.65 PTV.dial :PRVmixing @16:32:02.67 PSV.dial :mixloop @16:32:03.14 PS.reconfigure :default @16:32:03.19 PS.seek 0.3ml @16:32:07.11 delay 2 seconds @16:32:09.13 PRV.dial :air @16:32:09.65 PS.seek 0.55ml @16:32:13.11 addPMLReagent08 @16:32:13.12 PSV.dial :air @16:32:13.60 PS.seek 2ml @16:32:29.26 PSV.dial :PML @16:32:29.73 PTV.dial :mixing @16:32:30.48 PBV.dial :waste1 @16:32:30.92 PS.seek 1.85ml @16:32:33.36 PBV.dial between PSV and waste1 @16:32:33.72 PS.seek 0ml @16:32:53.64 delay 2 seconds @16:32:55.66 delay 8:00 @16:40:55.68 recoverPRVreagent08 @16:40:55.70 PTV.dial :mixing @16:40:55.77 PBV.dial :waste1 @16:40:56.13 delay 30 seconds @16:41:26.16 PBV.dial between PSV and waste1 @16:41:26.52 PSV.dial :air @16:41:26.99 PS.reconfigure :fast @16:41:27.04 PS.seek 2.5ml @16:41:50.75 PSV.dial :mixloop @16:41:51.22 delay 2 seconds @16:41:53.24 PSV.dial :air @16:41:53.71 delay 2 seconds @16:41:55.72 PSV.dial :mixloop @16:41:56.19 delay 2 seconds @16:41:58.21 PSV.dial :air @16:41:58.68 PS.seek 0ml @16:42:21.78 washCycleDA #1 of 3 @16:42:21.80 PSR.select :air @16:42:21.81 PSV.dial PSR @16:42:23.13 Pulling 0.5ml of PSR.wash @16:42:23.14 PSR.select :wash @16:42:23.26 PS.reconfigure :default @16:42:23.30 PS.seek 0.5ml @16:42:29.44 delay 5 seconds @16:42:34.46 PSR.select :air @16:42:34.58 PS.seek 2ml @16:42:50.78 PTV.dial :puck @16:42:51.52 PSV.dial PTV @16:42:52.25 PBV.dial :waste1 @16:42:52.69 PS.seek 1.5ml @16:42:58.63 delay 3 seconds @16:43:01.65 PBV.dial between PSV and waste1 @16:43:02.02 PS.seek 0ml @16:43:18.41 delay 2:00 @16:45:18.44 recoverPSRreagent08 @16:45:18.45 PTV.dial :puck @16:45:18.51 PBV.dial :waste1 @16:45:18.89 delay 30 seconds @16:45:48.92 PBV.dial between PSV and waste1 @16:45:49.73 PSR.select :air @16:45:49.75 PSV.dial PSR @16:45:50.63 PS.reconfigure :fast @16:45:50.68 PS.seek 2.5ml @16:46:14.25 PSV.dial PTV @16:46:14.98 delay 5 seconds @16:46:20.00 PSV.dial PSR @16:46:20.75 delay 5 seconds @16:46:25.77 PSV.dial PTV @16:46:26.65 delay 5 seconds @16:46:31.68 PSV.dial :air @16:46:32.44 PS.seek 0ml @16:46:55.53 washCycleDA #2 of 3 @16:46:55.54 PSR.select :air @16:46:55.56 PSV.dial PSR @16:46:56.87 Pulling 0.5ml of PSR.wash @16:46:56.88 PSR.select :wash @16:46:57.00 PS.reconfigure :default @16:46:57.04 PS.seek 0.5ml @16:47:03.03 delay 5 seconds @16:47:08.05 PSR.select :air @16:47:08.17 PS.seek 2ml @16:47:24.49 PTV.dial :puck @16:47:24.57 PSV.dial PTV @16:47:25.31 PBV.dial :waste1 @16:47:25.74 PS.seek 1.5ml @16:47:31.75 delay 3 seconds @16:47:34.76 PBV.dial between PSV and waste1 @16:47:35.14 PS.seek 0ml @16:47:51.46 delay 2:00 @16:49:51.49 recoverPSRreagent08 @16:49:51.50 PTV.dial :puck @16:49:51.52 PBV.dial :waste1 @16:49:51.88 delay 30 seconds @16:50:21.91 PBV.dial between PSV and waste1 @16:50:22.27 PSR.select :air @16:50:22.29 PSV.dial PSR @16:50:23.17 PS.reconfigure :fast @16:50:23.22 PS.seek 2.5ml @16:50:46.93 PSV.dial PTV @16:50:47.66 delay 5 seconds @16:50:52.69 PSV.dial PSR @16:50:53.45 delay 5 seconds @16:50:58.47 PSV.dial PTV @16:50:59.21 delay 5 seconds @16:51:04.23 PSV.dial :air @16:51:04.98 PS.seek 0ml @16:51:28.31 washCycleDA #3 of 3 @16:51:28.32 PSR.select :air @16:51:28.33 PSV.dial PSR @16:51:29.65 Pulling 0.5ml of PSR.wash @16:51:29.66 PSR.select :wash @16:51:29.78 PS.reconfigure :default @16:51:29.82 PS.seek 0.5ml @16:51:35.83 delay 5 seconds @16:51:40.86 PSR.select :air @16:51:40.98 PS.seek 2ml @16:51:57.18 PTV.dial :puck @16:51:57.19 PSV.dial PTV @16:51:57.93 PBV.dial :waste1 @16:51:58.35 PS.seek 1.5ml @16:52:04.30 delay 3 seconds @16:52:07.32 PBV.dial between PSV and waste1 @16:52:08.15 PS.seek 0ml @16:52:24.27 delay 2:00 @16:54:24.30 recoverPSRreagent08 @16:54:24.31 PTV.dial :puck @16:54:24.32 PBV.dial :waste1 @16:54:24.69 delay 30 seconds @16:54:54.72 PBV.dial between PSV and waste1 @16:54:55.08 PSR.select :air @16:54:55.10 PSV.dial PSR @16:54:55.85 PS.reconfigure :fast @16:54:55.90 PS.seek 2.5ml @16:55:19.55 PSV.dial PTV @16:55:20.30 delay 5 seconds @16:55:25.32 PSV.dial PSR @16:55:26.07 delay 5 seconds @16:55:31.09 PSV.dial PTV @16:55:31.83 delay 5 seconds @16:55:36.85 PSV.dial :air @16:55:37.60 PS.seek 0ml @16:56:00.92 Gate.power :camera,:ON @16:56:00.94 Mixing DA Substrates @16:56:00.95 PSR.select :air @16:56:00.96 PSV.dial PSR @16:56:02.33 Pulling 0.25ml of PSR.sub1 @16:56:02.35 PSR.select :sub1 @16:56:02.58 PS.reconfigure :default @16:56:02.69 PS.seek 0.25ml @16:56:06.18 delay 5 seconds @16:56:11.20 Pulling 0.25ml of PSR.sub2 @16:56:11.22 PSR.select :sub2 @16:56:11.33 PS.seek 0.5ml @16:56:14.70 delay 5 seconds @16:56:19.72 PSR.select :air @16:56:19.84 PS.seek 2ml @16:56:35.98 PTV.dial :puck @16:56:36.00 PSV.dial PTV @16:56:36.75 PBV.dial :waste1 @16:56:37.17 PS.seek 1.5ml @16:56:43.08 delay 3 seconds @16:56:46.10 PBV.dial between PSV and waste1 @16:56:46.46 PS.seek 0ml @16:57:02.61 delay 10 seconds @16:57:12.63 recoverPSRreagent08 @16:57:12.64 PTV.dial :puck @16:57:12.65 PBV.dial :waste1 @16:57:13.02 delay 30 seconds @16:57:43.04 PBV.dial between PSV and waste1 @16:57:43.40 PSR.select :air @16:57:43.42 PSV.dial PSR @16:57:44.19 PS.reconfigure :fast @16:57:44.24 PS.seek 2.5ml @16:58:08.36 PSV.dial PTV @16:58:09.25 delay 5 seconds @16:58:14.27 PSV.dial PSR @16:58:15.00 delay 5 seconds @16:58:20.02 PSV.dial PTV @16:58:20.78 delay 5 seconds @16:58:25.80 PSV.dial :air @16:58:26.58 PS.seek 0ml @16:58:50.17 Additional air clear @16:58:50.18 PSR.select :air @16:58:50.20 PSV.dial PSR @16:58:51.53 Pulling 2ml of PSR.air @16:58:51.54 PSR.select :air @16:58:51.56 PS.reconfigure :default @16:58:51.61 PS.seek 2ml @16:59:12.92 delay 5 seconds @16:59:17.94 PTV.dial :puck @16:59:17.96 PSV.dial PTV @16:59:18.70 PBV.dial :waste1 @16:59:19.59 PS.seek 1.5ml @16:59:25.51 delay 3 seconds @16:59:28.53 PBV.dial between PSV and waste1 @16:59:28.89 delay 1 second @16:59:29.91 PSV.dial :waste1 @16:59:30.97 PS.seek 0ml @16:59:47.13 Processing Puck Heater at 27.9C before imaging @16:59:47.14 PH.coast @16:59:47.25 Imaging da puck in Processing Clamp while storing it to tube 1 @16:59:47.43 SC.reconfigure :tight @16:59:47.49 SC.seek 1 @16:59:51.12 SC.configure :limp @16:59:51.33 Elbow.reconfigure :tight @16:59:51.38 Elbow.seek 1 @16:59:53.15 Forearm.reconfigure :tight @16:59:53.20 Forearm.seek 1 + 450 counts @16:59:54.28 SE.reconfigure :pick @16:59:54.34 SE.seek :beamBreak @16:59:57.39 SE.configure :default @16:59:57.56 Elbow.reconfigure :limp @16:59:57.61 Forearm.reconfigure :loose @16:59:57.66 Forearm.seek 1 @16:59:57.94 Elbow.hold @16:59:58.00 Elbow.reconfigure :tight @16:59:58.11 SE.seek 1.17 Pucks below Top Plate @16:59:58.54 Forearm.force -20 @16:59:58.58 Forearm.seek 1 + 302 counts @16:59:58.87 Forearm.reconfigure :tight @16:59:58.92 Forearm.seek 1 + 450 counts @16:59:59.11 SE.seek 2.17 Pucks below Top Plate @16:59:59.61 Forearm.reconfigure :loose @16:59:59.66 Forearm.seek :retract @17:00:00.58 Elbow.seek PC @17:00:03.08 Elbow.reconfigure :loose @17:00:03.60 Forearm.seek PC @17:00:04.61 Hand.close @17:00:05.21 PC.seek :open @17:00:10.00 Forearm.seek :retract @17:00:11.02 Elbow.reconfigure :tight @17:00:11.07 Elbow.seek 1 @17:00:13.67 Forearm.reconfigure :tight @17:00:13.72 Forearm.seek 1 + 450 counts @17:00:14.67 Hand.open @17:00:15.39 SE.seek 1.83 Pucks below Top Plate @17:00:15.67 SE.reconfigure :seal @17:00:15.72 SE.seek 0.86 Pucks below Top Plate @17:00:16.25 Lights, camera, ACTION! @17:00:16.28 PuckCamera: snap -bin=2,2 -auto /var/log/esp/da24jan1217h50ml.tif @17:00:24.28 SX HX9/16 (USB:02): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 3.545 seconds /var/log/esp/da24jan1217h50ml.tif: TIFF Upload Complete @17:00:24.31 PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da24jan1217h50ml15s.tif @17:00:35.77 Waiting >20 seconds for da to relinquish FlushPuck @17:00:39.94 SX HX9/16 (USB:02): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15 seconds /var/log/esp/midres/da24jan1217h50ml15s.tif: TIFF Upload Complete @17:00:39.97 PuckCamera: snap -bin=2,2 20.0 /var/log/esp/midres/da24jan1217h50ml20s.tif @17:01:00.69 SX HX9/16 (USB:02): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 20 seconds /var/log/esp/midres/da24jan1217h50ml20s.tif: TIFF Upload Complete @17:01:00.71 Gate.power :camera,:OFF @17:01:00.73 DAfans.email "3.545 sec auto-exposure",Subject: "da24jan1217h50ml images!" @17:01:01.18 Elbow.seek 1 @17:01:01.42 Forearm.seek 1 + 450 counts @17:01:01.50 SE.reconfigure :gently @17:01:01.57 SE.seek 1.58 Pucks below Top Plate @17:01:02.24 Elbow.reconfigure :limp @17:01:02.31 Forearm.reconfigure :loose @17:01:02.37 Forearm.seek 1 @17:01:02.64 Elbow.hold @17:01:02.71 Elbow.reconfigure :tight @17:01:02.76 SE.reconfigure :default @17:01:02.85 SE.seek 1.76 Pucks below Top Plate @17:01:03.22 Forearm.force -20 @17:01:03.26 Forearm.seek 1 + 303 counts @17:01:03.67 Forearm.reconfigure :tight @17:01:03.72 Forearm.seek 1 + 450 counts @17:01:04.82 SE.seek :down @17:01:06.10 Forearm.reconfigure :loose @17:01:06.20 Forearm.seek :retract @17:01:07.47 SE.stop @17:01:07.69 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @17:01:08.15 Elbow.seek FlushPuck::Garage @17:01:10.12 Elbow.reconfigure :loose @17:01:10.34 Forearm.seek :garage @17:01:11.42 Hand.close @17:01:12.38 Forearm.seek :retract @17:01:13.43 Elbow.reconfigure :tight @17:01:13.53 Elbow.seek PC @17:01:14.88 Elbow.reconfigure :loose @17:01:15.11 Forearm.reconfigure :tight @17:01:15.24 Forearm.seek PC @17:01:16.96 PC.seek :closed @17:01:21.48 upsync @17:01:21.69 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.62 @17:01:21.76 Hand.open @17:01:22.39 Forearm.reconfigure :loose @17:01:22.44 Forearm.seek :retract @17:01:23.39 PSR.select :air @17:01:23.41 PSV.dial PSR @17:01:23.87 Pulling 0.5ml of PSR.flush @17:01:23.88 PSR.select :flush @17:01:24.00 PS.seek 0.5ml @17:01:29.98 delay 5 seconds @17:01:35.00 PSR.select :air @17:01:36.13 PS.seek 2.5ml @17:01:57.42 delay 1 second @17:01:58.43 PRV.dial :waste2,avoiding: :ab2 @17:02:00.27 PTV.dial :PRVmixing @17:02:01.57 PSV.dial :mixloop @17:02:02.60 PS.seek :empty @17:02:29.04 PSR.select :air @17:02:29.06 PSV.dial PSR @17:02:30.09 Pulling 0.5ml of PSR.flush @17:02:30.10 PSR.select :flush @17:02:30.22 PS.seek 0.5ml @17:02:36.23 delay 5 seconds @17:02:41.25 PSR.select :air @17:02:41.37 PS.seek 2.5ml @17:03:02.63 delay 1 second @17:03:03.65 PBV.dial :waste1 @17:03:04.02 PTV.dial :mixing @17:03:04.77 PSV.dial :mixloop @17:03:05.82 PS.seek :empty @17:03:32.22 PSV.dial PSR @17:03:33.27 PSR.select :flush @17:03:33.39 PS.seek 1ml @17:03:44.44 PSR.select :air @17:03:44.56 PS.seek 2.5ml @17:04:00.69 PSV.dial PTV @17:04:01.57 PTV.dial :puck @17:04:02.30 PBV.dial :waste1 @17:04:02.39 PS.seek 0ml @17:04:28.78 PSV.dial PSR @17:04:29.52 PSR.select :flush @17:04:29.64 PS.seek 0.5ml @17:04:35.60 PSR.select :air @17:04:35.72 PS.seek 2.5ml @17:04:56.94 PSV.dial PTV @17:04:57.69 PBV.dial PSV @17:04:58.25 PS.seek 0ml @17:05:24.63 PSV.dial PBV @17:05:25.21 PS.seek 2.5ml @17:05:51.63 PSV.dial :waste1 @17:05:52.38 PS.seek 0ml @17:06:18.91 PSV.dial PSR @17:06:19.36 PSR.select :air @17:06:19.39 PS.seek 2.5ml @17:06:45.72 PSV.dial PTV @17:06:46.46 PBV.dial PSV @17:06:46.54 PS.seek 0ml @17:07:12.81 PSV.dial PBV @17:07:13.71 PS.seek 2.5ml @17:07:40.00 PSV.dial :waste1 @17:07:40.74 PS.seek 0ml @17:08:07.09 PSV.dial PSR @17:08:07.55 PSR.select :flush @17:08:07.67 PS.seek 1ml @17:08:18.75 PSR.select :air @17:08:18.87 PS.seek 2.5ml @17:08:35.03 PSV.dial PTV @17:08:35.89 PTV.dial :puck @17:08:35.97 PBV.dial :waste1 @17:08:36.52 PS.seek 0ml @17:09:03.09 PSV.dial PSR @17:09:03.83 PSR.select :flush @17:09:03.95 PS.seek 0.5ml @17:09:09.93 PSR.select :air @17:09:10.05 PS.seek 2.5ml @17:09:31.50 PSV.dial PTV @17:09:32.38 PBV.dial PSV @17:09:32.93 PS.seek 0ml @17:09:59.28 PSV.dial PBV @17:09:59.74 PS.seek 2.5ml @17:10:26.06 PSV.dial :waste1 @17:10:26.80 PS.seek 0ml @17:10:53.18 PSV.dial PSR @17:10:53.64 PSR.select :air @17:10:53.66 PS.seek 2.5ml @17:11:20.06 PSV.dial PTV @17:11:20.92 PBV.dial PSV @17:11:21.00 PS.seek 0ml @17:11:47.34 PSV.dial PBV @17:11:47.80 PS.seek 2.5ml @17:12:14.21 PSV.dial :waste1 @17:12:14.95 PS.seek 0ml @17:12:41.28 Garaging FlushPuck in Processing Clamp @17:12:41.35 Elbow.reconfigure :tight @17:12:41.40 Elbow.seek PC @17:12:41.52 Elbow.reconfigure :loose @17:12:41.62 Forearm.seek PC @17:12:42.58 Hand.close @17:12:43.20 PC.seek :open @17:12:48.40 Forearm.seek :retract @17:12:49.35 Elbow.reconfigure :tight @17:12:49.40 Elbow.seek :garage @17:12:50.75 Elbow.reconfigure :loose @17:12:50.85 Forearm.reconfigure :tight @17:12:50.90 Forearm.seek :garage @17:12:51.91 Hand.open @17:12:52.63 Forearm.reconfigure :loose @17:12:52.67 Forearm.seek :retract @17:50:10.99 Session Begun @17:50:14.55 -> CC Collection Clamp contains wcr puck from tube 2 @17:50:17.00 -> PC Processing Clamp open @17:50:19.26 -> Hand Hand is opened @17:50:22.41 -> Flush FlushPuck is in FlushPuck::Garage @17:51:42.69 -> MainThread.resume @17:51:42.71
Resuming Thread (suspended since 16:20:39) # @17:51:42.76 Pumping wcr puck from tube 2 up to 35psi... @17:51:42.83 CBV.dial SSV @17:51:44.25 CBV.dial between CSV and waste2b @17:51:45.21 CSV.dial between air and CTV @17:51:45.54 CS.reconfigure :fast @17:51:45.62 CS.seek 7.306ml @17:52:04.59 CTV.dial CSV,avoiding: :air @17:52:04.63
CSV.dial CTV @17:52:04.95 CS.reconfigure :pressurize @17:52:05.02 CS.seek 0ml @17:52:24.30 CSV.dial :air @17:52:24.78 CS.reconfigure :fast @17:52:24.85 CS.seek 10ml @17:52:49.30 CSV.dial between air and CTV @17:52:49.64 CS.seek 7.045ml @17:52:57.15 CTV.dial CSV,avoiding: :air @17:52:57.18
CSV.dial CTV @17:52:57.51 CS.reconfigure :pressurize @17:52:57.59 CS.seek 0ml @17:53:16.21 CSV.dial :air @17:53:16.68 CS.reconfigure :fast @17:53:16.75 CS.seek 10ml @17:53:41.76 CSV.dial between air and CTV @17:53:42.11 CS.seek 4.831ml @17:53:54.59 CTV.dial CSV,avoiding: :air @17:53:54.62
CSV.dial CTV @17:53:54.96 CS.reconfigure :pressurize @17:53:55.03 CS.seek 0ml @17:54:07.92 CSV.dial :air @17:54:08.40 CS.reconfigure :fast @17:54:08.47 CS.seek 10ml @17:54:32.77 CSV.dial between air and CTV @17:54:33.10 CS.seek 5.13ml @17:54:44.90 CTV.dial CSV,avoiding: :air @17:54:44.93
CSV.dial CTV @17:54:45.26 CS.reconfigure :pressurize @17:54:45.34 CS.seek 0ml @17:54:58.99 CSV.dial :air @17:54:59.46 CS.reconfigure :default @17:54:59.52 CS.hold @17:54:59.67 ShallowSampler::Error in MAIN -- Puck.from 2, :wcr reached only 24.5 of 35psi after pumping in 40ml of gas @17:54:59.69 Waiting to Resume from Checkpoint "Pumping wcr puck from tube 2 up to 35psi..." @17:54:59.72 BadNews.email "Puck.from 2, :wcr reached only 24.5 of 35psi after pumping in 40ml of gas",Subject: "ShallowSampler::Error in MAIN" @17:55:03.76 upsync @17:55:37.86 -> CC.unloadPuck 1, ;WCR @17:55:37.88 SyntaxError in dave -- compile error dave:6: syntax error, unexpected ';' CC.unloadPuck 1, ;WCR ^ @17:55:51.46 -> CC.unloadPuck 1, :WCR @17:55:51.55 Puck::Warning in dave -- Unloading Puck.from 2, :wcr to Storage Carousel @17:55:51.57 Storing WCR puck in Collection Clamp to tube 1 @17:55:51.79 SC.reconfigure :tight @17:55:51.86 SC.seek 1 @17:55:52.09 SC.configure :limp @17:55:52.32 Elbow.reconfigure :tight @17:55:52.40 Elbow.seek 1 @17:55:54.26 Forearm.reconfigure :tight @17:55:54.34 Forearm.seek 1 + 450 counts @17:55:55.39 SE.reconfigure :pick @17:55:55.94 SE.seek :beamBreak @17:55:58.90 SE.configure :default @17:55:59.08 Elbow.reconfigure :limp @17:55:59.15 Forearm.reconfigure :loose @17:55:59.22 Forearm.seek 1 @17:55:59.47 Elbow.hold @17:55:59.55 Elbow.reconfigure :tight @17:55:59.67 SE.seek 2.16 Pucks below Top Plate @17:56:00.14 Forearm.force -20 @17:56:00.19 Forearm.seek 1 + 296 counts @17:56:00.49 Forearm.reconfigure :tight @17:56:00.56 Forearm.seek 1 + 450 counts @17:56:00.77 SE.seek 3.16 Pucks below Top Plate @17:56:01.29 Forearm.reconfigure :loose @17:56:01.36 Forearm.seek :retract @17:56:02.31 Elbow.seek CC @17:56:03.98 Elbow.reconfigure :loose @17:56:04.09 Forearm.seek CC @17:56:05.08 Hand.close @17:56:05.66 CC.seek :open @17:56:10.40 Forearm.seek :retract @17:56:11.44 Elbow.reconfigure :tight @17:56:11.51 Elbow.seek 1 @17:56:13.28 Elbow.reconfigure :limp @17:56:13.35 Forearm.seek 1 @17:56:14.31 Elbow.hold @17:56:14.39 Elbow.reconfigure :tight @17:56:14.46 Hand.open @17:56:15.14 Forearm.force -20 @17:56:15.21 Forearm.seek 1 + 316 counts @17:56:15.62 Forearm.reconfigure :tight @17:56:15.69 Forearm.seek 1 + 450 counts @17:56:15.94 SE.seek :down @17:56:17.06 Forearm.reconfigure :loose @17:56:17.13 Forearm.seek :retract @17:56:18.47 SE.stop @17:56:26.08 -> Puck.count @17:56:26.17 SE.seek :down @17:56:26.26 SE.stop @17:56:26.37 SC.reconfigure :tight @17:56:26.44 SC.seek 1 @17:56:26.52 SC.configure :limp @17:56:26.70 Elbow.seek 1 @17:56:26.91 Forearm.reconfigure :tight @17:56:26.99 Forearm.seek 1 + 450 counts @17:56:28.03 SE.reconfigure :place @17:56:28.11 SE.seek :beamBreak @17:56:31.24 SE.configure :default @17:56:31.37 SE.seek :down @17:56:32.50 Forearm.reconfigure :loose @17:56:32.58 Forearm.seek :retract @17:56:33.81 SE.stop @17:56:33.87 SC.reconfigure :tight @17:56:33.94 SC.seek 2 @17:56:37.51 SC.configure :limp @17:56:37.69 Elbow.seek 2 @17:56:37.89 Forearm.reconfigure :tight @17:56:37.97 Forearm.seek 2 + 450 counts @17:56:39.03 SE.reconfigure :place @17:56:39.11 SE.seek :beamBreak @17:56:41.99 SE.configure :default @17:56:42.12 SE.seek :down @17:56:43.25 Forearm.reconfigure :loose @17:56:43.32 Forearm.seek :retract @17:56:44.94 SE.stop @17:56:45.00 SC.reconfigure :tight @17:56:45.07 SC.seek 3 @17:56:48.51 SC.configure :limp @17:56:48.69 Elbow.seek 3 @17:56:49.17 Forearm.reconfigure :tight @17:56:49.25 Forearm.seek 3 + 450 counts @17:56:50.38 SE.reconfigure :place @17:56:50.46 SE.seek :beamBreak @17:56:53.77 SE.configure :default @17:56:53.90 SE.seek :down @17:56:55.03 Forearm.reconfigure :loose @17:56:55.10 Forearm.seek :retract @17:56:56.70 SE.stop @17:56:56.77 SC.reconfigure :tight @17:56:56.84 SC.seek 4 @17:57:00.43 SC.configure :limp @17:57:00.60 Elbow.seek 4 @17:57:01.08 Forearm.reconfigure :tight @17:57:01.16 Forearm.seek 4 + 450 counts @17:57:02.17 SE.reconfigure :place @17:57:02.25 SE.seek :beamBreak @17:57:05.10 SE.configure :default @17:57:05.23 SE.seek :down @17:57:06.36 Forearm.reconfigure :loose @17:57:06.43 Forearm.seek :retract @17:57:08.05 SE.stop @17:57:08.11 SC.reconfigure :tight @17:57:08.18 SC.seek 5 @17:57:11.65 SC.configure :limp @17:57:11.83 Elbow.seek 5 @17:57:12.10 Forearm.reconfigure :tight @17:57:12.17 Forearm.seek 5 + 450 counts @17:57:13.23 SE.reconfigure :place @17:57:13.31 SE.seek :beamBreak @17:57:16.15 SE.configure :default @17:57:16.28 SE.seek :down @17:57:17.42 Forearm.reconfigure :loose @17:57:17.50 Forearm.seek :retract @17:57:19.09 SE.stop @17:57:19.15 SC.reconfigure :tight @17:57:19.22 SC.seek 6 @17:57:22.78 SC.configure :limp @17:57:22.95 Elbow.seek 6 @17:57:23.54 Forearm.reconfigure :tight @17:57:23.69 Forearm.seek 6 + 450 counts @17:57:24.68 SE.reconfigure :place @17:57:24.76 SE.seek :beamBreak @17:57:27.62 SE.configure :default @17:57:27.75 SE.seek :down @17:57:28.88 Forearm.reconfigure :loose @17:57:28.95 Forearm.seek :retract @17:57:30.58 SE.stop @17:57:30.64 SC.reconfigure :tight @17:57:30.71 SC.seek 7 @17:57:34.25 SC.configure :limp @17:57:34.43 Elbow.seek 7 @17:57:34.87 Forearm.reconfigure :tight @17:57:34.94 Forearm.seek 7 + 450 counts @17:57:36.01 SE.reconfigure :place @17:57:36.09 SE.seek :beamBreak @17:57:38.93 SE.configure :default @17:57:39.06 SE.seek :down @17:57:40.19 Forearm.reconfigure :loose @17:57:40.26 Forearm.seek :retract @17:57:41.89 SE.stop {1 => 4, 2 => 0, 3 => 0, 4 => 0, 5 => 0, 6 => 0, 7 => 0} @17:58:27.08 -> exit @17:58:27.09 Session Ended + ESPwhich standAloneWCR_010224.rb + fn=standAloneWCR_010224.rb + IFS=: + set /home/esp/esp2/mission . + fullPath=/home/esp/esp2/mission/standAloneWCR_010224.rb + [ -f /home/esp/esp2/mission/standAloneWCR_010224.rb ] + realpath /home/esp/esp2/mission/standAloneWCR_010224.rb + return 0 + scriptPath=/home/esp/esp2local/mission/standAloneWCR_010224.rb + exec ruby /home/esp/esp2/lib/esp.rb /home/esp/esp2local/mission/standAloneWCR_010224.rb @14:33:33.39UTC16-Jan-24
ESP2 v3.42 I2C Gateway v3.20 for core bus @14:33:36.02 Configuration for short puck jake 1/11/21 by brent@mbari.org /home/esp/esp2/type/shallow/jake/configure.rb @14:33:39.66 Polling Can every 10:00 @14:33:39.71 Loading /home/esp/esp2local/mission/standAloneWCR_010224.rb mission @14:33:39.77 Preparing to sleep until + 00:00:05 @14:33:39.79 GoodNews.email "Starting standAloneWCR_010224 w/pucks in tube 2\n{2 => 1}\nCTD data unavailable\nCan@14:33:39, 26% flow, 21.7C, 20% humidity, 14.9psia, 13.842V, 0.302A, 41.907Ah, 4.19W",Subject: "standAloneWCR_010224 begun" @14:33:39.85
Asleep til + 00:00:05 @14:33:39.87 Continuing at 14:33 @14:33:40.13 Gate.power :core,:OFF @14:33:40.14 Gate.power :raw,:OFF @14:33:40.15 Shutdown @14:33:43.41 GoodNews.email "Then, in tube 2: initialPurge; standAloneWCR([nil,100], $da)\n/home/esp/esp2local/mission/standAloneWCR_010224.rb:10\n{2 => 1}\nCTD data unavailable\nCan@14:33:39, 26% flow, 21.6C, 20% humidity, 14.9psia, 13.847V, 0.302A, 41.907Ah, 4.19W",Subject: "Asleep until 14:33" @14:33:44.78
Powering core bus @14:33:44.88 upsync @14:33:47.38 upsync @14:33:50.21
Gate.power :raw,:ON @14:33:51.65 CS.configure :default @14:33:51.70 PS.configure :default @14:33:51.75 SS.configure :default @14:33:51.80 CC.configure :default @14:33:51.84 PC.configure :default @14:33:51.91 Forearm.configure :loose @14:33:51.96 Elbow.configure :loose @14:33:52.03 SC.configure :loose @14:33:52.08 SE.configure :default @14:33:52.13 CH.configure :default @14:33:52.17 PH.configure :default @14:33:52.20 All dwarf controllers running firmware version 3.85 @14:33:52.31 Valve.reset @14:33:52.54 Intake.select :close @14:33:52.59 Exhaust.select :close @14:33:52.63 CTV.dial :air @14:33:52.70 PSV.dial :air @14:33:52.82
Forearm.home.seek :retract @14:33:53.58 Hand.close @14:33:53.61
Elbow.reconfigure :loose @14:33:53.70 Elbow.jog 1500 @14:33:53.91 PS.home.seek 0ml @14:33:53.97 CSV.dial :waste1 @14:33:54.11
Elbow.reconfigure :loose @14:33:54.16 Elbow.home.seek :clear @14:33:54.70 SSV.dial :spit,avoiding: :exhaust @14:33:55.56 CS.reconfigure :fast @14:33:55.60 SS.home.seek 0ml @14:33:55.68 CS.home.seek 0ml @14:33:57.35
SE.home.seek :down @14:33:57.96 SC.reconfigure :tight @14:33:58.01 SC.home.seek :initial @14:34:02.21 PS.stop @14:34:23.21
SC.configure :limp @14:34:23.26 Forearm.seek :retract @14:34:23.33 Elbow.seek :hammer @14:34:24.33 Forearm.reconfigure :tight @14:34:24.38 Forearm.seek retracted @14:34:24.70 PC.home.seek :open @14:34:24.73
CC.home.seek :open @14:34:28.17 Phase [initialPurge; standAloneWCR([nil,100], $da)] @14:34:28.18 Notice.email "At 14:34:28 began in tube 2: initialPurge; standAloneWCR([nil,100], $da)\n/home/esp/esp2local/mission/standAloneWCR_010224.rb:10\n{2 => 1}\nCTD data unavailable\nCan@14:34:28, 25% flow, 21.7C, 20% humidity, 14.9psia, 13.803V, 0.290A, 41.912Ah, 4.01W",Subject: "Started standAloneWCR_010224 phase initialPurge; standAloneWCR([nil,100], $da)" @14:34:28.20
Starting initialPurge @14:34:28.24 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:34:28.30 Hand.open @14:34:29.09 Elbow.reconfigure :tight @14:34:29.16 Elbow.seek FlushPuck::Garage @14:34:31.06 Elbow.reconfigure :loose @14:34:31.18 Forearm.reconfigure :loose @14:34:31.23 Forearm.seek :garage @14:34:31.90 upsync @14:34:32.20
Hand.close @14:34:32.96 Forearm.seek :retract @14:34:33.92 Elbow.reconfigure :tight @14:34:33.97 Elbow.seek CC @14:34:36.91 Elbow.reconfigure :loose @14:34:37.01 Forearm.reconfigure :tight @14:34:37.07 Forearm.seek CC @14:34:38.30 CC.seek :closed @14:34:42.92 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.28 @14:34:42.99 Hand.open @14:34:43.65 Forearm.reconfigure :loose @14:34:43.70 Forearm.seek :retract @14:34:44.67 Flushing the Collection Valve::Manifold @14:34:44.79 CSV.dial CSR @14:34:46.06 Pulling 5ml of CSR.flush CSR.select :flush @14:34:46.18 CS.reconfigure :default @14:34:46.23 CS.seek 5ml @14:35:00.07 delay 5 seconds @14:35:05.09 CSR.select :air @14:35:05.67 CS.reconfigure :fast @14:35:05.71 CS.seek 7.5ml @14:35:12.48 CSV.dial :waste @14:35:13.76 CS.reconfigure :default @14:35:13.81 CS.seek 0ml @14:35:33.82 Purging external Intake @14:35:33.94 CSV.dial :flush @14:35:34.40 CS.reconfigure :fast @14:35:34.45 CS.seek 10ml @14:35:59.16 delay 5 seconds @14:36:04.19 CSV.dial CBV @14:36:04.22
CBV.dial CSV @14:36:04.99 CTV.dial :intake @14:36:05.77 Intake.select :open @14:36:05.89 CS.seek 0ml @14:36:29.35 Intake.select :close @14:36:29.46 CTV.dial :air @14:36:30.25 Flushing upper manifold @14:36:30.37 CSV.dial :flush @14:36:31.09 CS.seek 5ml @14:36:43.85 delay 5 seconds @14:36:48.87 CSV.dial :air @14:36:49.36 CS.seek 7ml @14:36:54.95 CSV.dial CBV @14:36:54.97
CBV.dial CSV @14:36:55.98 CTV.dial :air @14:36:56.06 CS.seek 0ml @14:37:12.69 delay 7 seconds @14:37:19.72 CS.seek 3ml @14:37:27.85 CSV.dial :waste @14:37:28.31 CS.seek 0ml @14:37:36.24 CSV.dial :flush @14:37:36.68 CS.seek 3ml @14:37:44.91 delay 5 seconds @14:37:49.93 CSV.dial :air @14:37:50.44 CS.seek 5ml @14:37:55.94 CSV.dial :air @14:37:56.12 CS.seek 0ml @14:38:08.22 delay 7 seconds @14:38:15.25 CS.seek 3ml @14:38:23.32 CSV.dial :waste @14:38:24.04 CS.seek 0ml @14:38:31.69 Garaging FlushPuck in Collection Clamp @14:38:31.76 Elbow.reconfigure :tight @14:38:31.81 Elbow.seek CC @14:38:32.05 Elbow.reconfigure :loose @14:38:32.15 Forearm.seek CC @14:38:33.10 Hand.close @14:38:33.72 CC.seek :open @14:38:38.74 Forearm.seek :retract @14:38:39.76 Elbow.reconfigure :tight @14:38:39.81 Elbow.seek :garage @14:38:42.74 Elbow.reconfigure :loose @14:38:42.84 Forearm.reconfigure :tight @14:38:42.88 Forearm.seek :garage @14:38:43.83 Hand.open @14:38:44.52 Forearm.reconfigure :loose @14:38:44.57 Forearm.seek :retract @14:38:45.52 Starting stand-alone da WCR @14:38:45.53 da wcr sampling at most 100ml @14:38:45.57 Whole Cell Archive sampling 100ml @14:38:45.58 DAfans.email "Started stand-alone da WCR in tube 2 at 14:38:45UTC16-Jan-24\n{2 => 1}\nCTD data unavailable\nCan@14:34:28, 25% flow, 21.7C, 20% humidity, 14.9psia, 13.803V, 0.290A, 41.912Ah, 4.01W",Subject: "da wcr sampling at most 100ml" @14:38:45.62 Loading wcr puck from tube 2 into Collection Clamp @14:38:45.89 SC.reconfigure :tight @14:38:45.94 SC.seek 2 @14:38:49.42 upsync @14:38:52.71 SC.configure :limp @14:38:52.94 Elbow.reconfigure :tight @14:38:53.00 Elbow.seek 2 @14:38:54.91 Forearm.reconfigure :tight @14:38:54.97 Forearm.seek 2 + 450 counts @14:38:56.04 SE.reconfigure :pick @14:38:56.11 SE.seek :beamBreak @14:38:59.04 SE.configure :default @14:38:59.11 SE.seek :down @14:39:01.63 SE.stop @14:39:01.74 Forearm.reconfigure :loose @14:39:01.80 Forearm.seek :retract @14:39:02.79 Puck::Error in MAIN -- 4 pucks loaded in tube 2 when expecting 1 @14:39:02.81 BadNews.email "4 pucks loaded in tube 2 when expecting 1",Subject: "Puck::Error in MAIN" @14:39:02.82
Waiting to Resume from Checkpoint "Loading wcr puck from tube 2 into Collection Clamp" @14:39:06.84 upsync + ESPwhich DA_WCR_011024.rb + fn=DA_WCR_011024.rb + IFS=: + set /home/esp/esp2/mission . + fullPath=/home/esp/esp2/mission/DA_WCR_011024.rb + [ -f /home/esp/esp2/mission/DA_WCR_011024.rb ] + realpath /home/esp/esp2/mission/DA_WCR_011024.rb + return 0 + scriptPath=/home/esp/esp2local/mission/DA_WCR_011024.rb + exec ruby /home/esp/esp2/lib/esp.rb /home/esp/esp2local/mission/DA_WCR_011024.rb Log::Locked in trapHandler -- Another process is already writing to /var/log/esp/real.log + ESPwhich DA_WCR_011024.rb + fn=DA_WCR_011024.rb + IFS=: + set /home/esp/esp2/mission . + fullPath=/home/esp/esp2/mission/DA_WCR_011024.rb + [ -f /home/esp/esp2/mission/DA_WCR_011024.rb ] + realpath /home/esp/esp2/mission/DA_WCR_011024.rb + return 0 + scriptPath=/home/esp/esp2local/mission/DA_WCR_011024.rb + exec ruby /home/esp/esp2/lib/esp.rb /home/esp/esp2local/mission/DA_WCR_011024.rb @14:46:29.93UTC16-Jan-24
ESP2 v3.42 I2C Gateway v3.20 for core bus @14:46:32.51 Configuration for short puck jake 1/11/21 by brent@mbari.org /home/esp/esp2/type/shallow/jake/configure.rb @14:46:36.40 Polling Can every 10:00 @14:46:36.42 Cleanly exited at 14:46:13UTC16-Jan-24 @14:46:36.46 Loading /home/esp/esp2local/mission/DA_WCR_011024.rb mission @14:46:36.51 GoodNews.email "Starting DA_WCR_011024 w/pucks in tube 2\n{2 => 1}\nCTD data unavailable\nCan@14:46:36, 25% flow, 21.7C, 20% humidity, 14.9psia, 13.851V, 0.272A, 41.979Ah, 3.77W",Subject: "DA_WCR_011024 begun" @14:46:36.56
Preparing to sleep until + 00:00:05 @14:46:36.59 Asleep til + 00:00:05 @14:46:36.60 Continuing at 14:46 @14:46:36.89 Gate.power :core,:OFF @14:46:36.90 Gate.power :raw,:OFF @14:46:36.91 Shutdown @14:46:38.95 GoodNews.email "Then, in tube 2: initialPurge; da [50, 50] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_011024.rb:14\n{1 => 0,\n 2 => 4,\n 3 => 0,\n 4 => 0,\n 5 => 0,\n 6 => 0,\n 7 => 0}\nCTD data unavailable\nCan@14:46:36, 25% flow, 21.7C, 20% humidity, 14.9psia, 13.851V, 0.272A, 41.979Ah, 3.77W",Subject: "Asleep until 14:46" @14:46:40.40 upsync @14:46:41.58
Powering core bus @14:46:42.85 upsync @14:46:46.95
Gate.power :raw,:ON @14:46:47.66 CS.configure :default @14:46:47.70 PS.configure :default @14:46:47.75 SS.configure :default @14:46:47.80 CC.configure :default @14:46:47.85 PC.configure :default @14:46:47.92 Forearm.configure :loose @14:46:47.97 Elbow.configure :loose @14:46:48.04 SC.configure :loose @14:46:48.09 SE.configure :default @14:46:48.14 CH.configure :default @14:46:48.17 PH.configure :default @14:46:48.21 All dwarf controllers running firmware version 3.85 @14:46:48.32 Valve.reset @14:46:48.53 Intake.select :close @14:46:48.61 Exhaust.select :close @14:46:48.64 CTV.dial :air @14:46:49.10 PSV.dial :air @14:46:49.24 CSV.dial :waste1 @14:46:49.29 PS.home.seek 0ml @14:46:49.31
Forearm.home.seek :retract @14:46:49.44 SSV.dial :spit,avoiding: :exhaust @14:46:49.62 CS.reconfigure :fast @14:46:49.66 SS.home.seek 0ml @14:46:49.75 CS.home.seek 0ml @14:46:50.07 Hand.close @14:46:50.11
Elbow.reconfigure :loose @14:46:50.19 Elbow.jog 1500 @14:46:50.70 Elbow.reconfigure :loose @14:46:50.76 Elbow.home.seek :clear @14:46:55.20 SE.home.seek :down @14:46:55.82 SC.reconfigure :tight @14:46:55.86 SC.home.seek :initial @14:46:57.66 PS.stop @14:47:08.39
SC.configure :limp @14:47:08.44 Forearm.seek :retract @14:47:08.51 Elbow.seek :hammer @14:47:09.54 Forearm.reconfigure :tight @14:47:09.58 Forearm.seek retracted @14:47:09.85 PC.home.seek :open @14:47:09.86
CC.home.seek :open @14:47:13.26 Phase [initialPurge; da [50, 50] #da and wsr are run simultanelously] @14:47:13.28 Notice.email "At 14:47:13 began in tube 2: initialPurge; da [50, 50] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_011024.rb:14\n{1 => 0,\n 2 => 4,\n 3 => 0,\n 4 => 0,\n 5 => 0,\n 6 => 0,\n 7 => 0}\nCTD data unavailable\nCan@14:47:13, 25% flow, 21.8C, 20% humidity, 14.9psia, 13.837V, 0.290A, 41.982Ah, 4.02W",Subject: "Started DA_WCR_011024 phase initialPurge; da [50, 50] #da and wsr are run simultanelously" @14:47:13.30
Starting initialPurge @14:47:13.32 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:47:13.41 Hand.open @14:47:14.12 Elbow.reconfigure :tight @14:47:14.20 Elbow.seek FlushPuck::Garage @14:47:16.14 Elbow.reconfigure :loose @14:47:16.25 Forearm.reconfigure :loose @14:47:16.31 Forearm.seek :garage @14:47:17.15 upsync @14:47:17.31
Hand.close @14:47:18.01 Forearm.seek :retract @14:47:19.01 Elbow.reconfigure :tight @14:47:19.06 Elbow.seek CC @14:47:21.97 Elbow.reconfigure :loose @14:47:22.07 Forearm.reconfigure :tight @14:47:22.12 Forearm.seek CC @14:47:23.40 CC.seek :closed @14:47:27.98 Collection Clamp onSpring/offSpring for 14/27 samples, on/off torque 2.22 @14:47:28.05 Hand.open @14:47:28.69 Forearm.reconfigure :loose @14:47:28.74 Forearm.seek :retract @14:47:29.71 Flushing the Collection Valve::Manifold @14:47:29.84 CSV.dial CSR @14:47:31.13 Pulling 5ml of CSR.flush @14:47:31.14 CSR.select :flush @14:47:31.26 CS.reconfigure :default @14:47:31.30 CS.seek 5ml @14:47:45.08 delay 5 seconds @14:47:50.44 CSR.select :air @14:47:50.67 CS.reconfigure :fast @14:47:50.72 CS.seek 7.5ml @14:47:57.42 CSV.dial :waste @14:47:58.69 CS.reconfigure :default @14:47:58.74 CS.seek 0ml @14:48:18.71 Purging external Intake @14:48:18.83 CSV.dial :flush @14:48:19.29 CS.reconfigure :fast @14:48:19.33 CS.seek 10ml @14:48:43.77 delay 5 seconds @14:48:48.79 CSV.dial CBV @14:48:48.82
CBV.dial CSV @14:48:49.58 CTV.dial :intake @14:48:50.36 Intake.select :open @14:48:50.47 CS.seek 0ml @14:49:13.98 Intake.select :close @14:49:14.09 CTV.dial :air @14:49:14.86 Flushing upper manifold @14:49:14.98 CSV.dial :flush @14:49:15.71 CS.seek 5ml @14:49:28.36 delay 5 seconds @14:49:33.38 CSV.dial :air @14:49:33.89 CS.seek 7.001ml @14:49:39.46 CSV.dial CBV @14:49:39.48
CBV.dial CSV @14:49:40.50 CTV.dial :air @14:49:40.58 CS.seek 0ml @14:49:57.11 delay 7 seconds @14:50:04.14 CS.seek 3ml @14:50:12.20 CSV.dial :waste @14:50:12.66 CS.seek 0ml @14:50:20.49 CSV.dial :flush @14:50:20.94 CS.seek 3ml @14:50:28.98 delay 5 seconds @14:50:34.00 CSV.dial :air @14:50:34.50 CS.seek 5ml @14:50:40.11 CSV.dial :air @14:50:40.28 CS.seek 0ml @14:50:52.33 delay 7 seconds @14:50:59.35 CS.seek 3ml @14:51:07.42 CSV.dial :waste @14:51:08.14 CS.seek 0ml @14:51:15.77 Garaging FlushPuck in Collection Clamp @14:51:15.83 Elbow.reconfigure :tight @14:51:15.88 Elbow.seek CC @14:51:16.12 Elbow.reconfigure :loose @14:51:16.22 Forearm.seek CC @14:51:17.17 Hand.close @14:51:17.75 CC.seek :open @14:51:22.78 Forearm.seek :retract @14:51:23.82 Elbow.reconfigure :tight @14:51:23.86 Elbow.seek :garage @14:51:26.82 Elbow.reconfigure :loose @14:51:26.92 Forearm.reconfigure :tight @14:51:26.97 Forearm.seek :garage @14:51:27.92 Hand.open @14:51:28.56 Forearm.reconfigure :loose @14:51:28.61 Forearm.seek :retract @14:51:29.58 da sampling at most 50ml, wcr at most 50ml @14:51:29.62 Loading sh1 puck from tube 2 into Collection Clamp @14:51:29.63 DAfans.email "Started da in tube 2 at 14:51:29UTC16-Jan-24\n{1 => 0,\n 2 => 4,\n 3 => 0,\n 4 => 0,\n 5 => 0,\n 6 => 0,\n 7 => 0}\nCTD data unavailable\nCan@14:47:13, 25% flow, 21.8C, 20% humidity, 14.9psia, 13.837V, 0.290A, 41.982Ah, 4.02W",Subject: "da sampling at most 50ml, wcr at most 50ml" @14:51:29.90
SC.reconfigure :tight @14:51:29.97 SC.seek 2 @14:51:33.46 upsync @14:51:36.69
SC.configure :limp @14:51:36.92 Elbow.reconfigure :tight @14:51:36.97 Elbow.seek 2 @14:51:38.90 Forearm.reconfigure :tight @14:51:38.95 Forearm.seek 2 + 450 counts @14:51:40.00 SE.reconfigure :pick @14:51:40.07 SE.seek :beamBreak @14:51:42.95 SE.configure :default @14:51:43.12 Elbow.seek 2 @14:51:43.31 Forearm.seek 2 + 300 counts @14:51:43.56 Forearm.reconfigure :loose @14:51:43.61 Hand.close @14:51:44.27 SE.seek :down @14:51:44.47
Forearm.seek :retract @14:51:45.43 Elbow.seek CC @14:51:46.84 SE.stop @14:51:47.38
Elbow.reconfigure :loose @14:51:47.49 Forearm.reconfigure :tight @14:51:47.54 Forearm.seek CC @14:51:48.83 CC.seek :closed @14:51:53.35 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.10 @14:51:53.36 Hand.open @14:51:54.06 Forearm.reconfigure :loose @14:51:54.11 Forearm.seek :retract @14:51:55.16 collectSample 50ml w/bubble point of 24psi @14:51:55.17 Priming 7ml @14:51:55.18 Intake.select :close @14:51:55.19 Exhaust.select :close @14:51:55.21 SS.hold @14:51:55.42 Calibrating pressure and temperature sensors @14:51:55.45 CTV.dial :air @14:51:55.54 SSV.dial :spit,avoiding: :exhaust @14:51:55.63 SS.reconfigure :fast @14:51:55.67 SS.seek 5ml @14:51:58.53 SSV.dial CBV @14:51:58.57
CBV.dial SSV @14:51:59.87 delay 3.8 seconds @14:52:03.80 Sensor limits: Intake max 111.4psia; Outlet max 106.0psia @14:52:03.81 SSV.dial :spit @14:52:05.11 SS.seek 0ml @14:52:07.93 SSV.dial CBV @14:52:09.25 CTV.dial :intake @14:52:10.03 SSV.dial CBV @14:52:10.05
CBV.dial SSV @14:52:10.25 Allowing 40s for 7.0ml of remaining 7.0ml @14:52:10.26 Intake.select :open @14:52:12.42 Intake @15.5psia, vacuum from 10.0 to 13.0psi @14:52:12.44 SS.reconfigure :inhaling 2.20ml/s @14:52:12.49 SS.seek 7ml @14:52:19.30 Stroke completed -- waiting for pressure to equalize ... @14:52:19.31 SS.reconfigure :exhale @14:52:19.36 SS.hold @14:52:22.16 Intake.select :close @14:52:22.31 SSV.dial :exhaust @14:52:23.06 Exhaust.select :open @14:52:23.17 SS.seek 0ml @14:52:27.18 Exhaust.select :close @14:52:27.29 Sampling 50.0ml @14:52:27.30 Intake.select :close @14:52:27.31 Exhaust.select :close @14:52:27.32 SS.reconfigure :default @14:52:27.36 SS.hold @14:52:27.56 CTV.dial :intake @14:52:27.75 SSV.dial CBV @14:52:27.78
CBV.dial SSV @14:52:28.54 Allowing 130s for 25.0ml of remaining 50.0ml @14:52:28.55 Intake.select :open @14:52:30.72 Intake @15.6psia, vacuum from 10.0 to 13.0psi @14:52:30.73 SS.reconfigure :inhaling 2.20ml/s @14:52:30.78 SS.seek 25ml @14:52:45.88 Stroke completed -- waiting for pressure to equalize ... @14:52:45.89 SS.reconfigure :exhale @14:52:45.94 SS.hold @14:52:49.37 Intake.select :close @14:52:49.53 SSV.dial :exhaust @14:52:50.27 Exhaust.select :open @14:52:50.39 SS.seek 0ml @14:52:59.24 Exhaust.select :close @14:52:59.35 SSV.dial CBV @14:52:59.38
CBV.dial SSV @14:53:00.13 Allowing 130s for 25.0ml of remaining 25.0ml @14:53:00.14 Intake.select :open @14:53:02.31 Intake @15.5psia, vacuum from 10.0 to 13.0psi @14:53:02.33 SS.reconfigure :inhaling 2.20ml/s @14:53:02.38 SS.seek 25ml @14:53:17.71 Stroke completed -- waiting for pressure to equalize ... @14:53:17.72 SS.reconfigure :exhale @14:53:17.76 SS.hold @14:53:21.71 Intake.select :close @14:53:21.86 SSV.dial :exhaust @14:53:22.59 Exhaust.select :open @14:53:22.70 SS.seek 0ml @14:53:31.52 Exhaust.select :close @14:53:31.63 Sampled 50.0ml @14:53:31.65 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @14:53:31.66 Intake.select :close @14:53:31.72 CSV.dial :waste @14:53:31.89 CS.seek 0ml @14:53:31.92 SSV.dial CBV,avoiding: :exhaust @14:53:31.95
CBV.dial SSV @14:53:32.08 CSV.dial :air @14:53:32.80 CS.seek 10ml @14:53:33.84
CTV.dial :air @14:53:34.59 delay 15 seconds @14:53:49.61 SSV.dial :spit,avoiding: :exhaust @14:53:50.90 CTV.dial CSV,avoiding: :intake @14:53:50.94
SS.seek 0ml @14:53:51.07 SSV.dial CBV,avoiding: :exhaust @14:53:57.17 SSV.dial between CBV and plug @14:53:57.64 CBV.dial between SSV and waste2 @14:53:58.03 CTV.dial CSV,avoiding: :air @14:53:58.06
CSV.dial CTV @14:53:58.58 CS.reconfigure :pressurize @14:53:58.63 CS.seek 0ml @14:54:16.74 BubblePoint: Intake @24.2psia, Outlet @0.7psia @14:54:16.75 CBV.dial SSV @14:54:17.08 SS.reconfigure :default @14:54:17.13 SS.seek 10ml @14:54:22.82 delay 15 seconds @14:54:37.95 Evacuated: Intake @23.9psia, Outlet @24.0psia @14:54:37.96 SSV.dial :spit,avoiding: :exhaust @14:54:39.00 SS.reconfigure :exhale @14:54:39.04 SS.seek 0ml @14:54:44.32 SS.reconfigure :fast @14:54:44.37 SS.seek 5ml @14:54:47.28 SSV.dial CBV,avoiding: :exhaust @14:54:47.31
CBV.dial SSV @14:54:48.62 CTV.dial :air,avoiding: :intake @14:54:49.92 CSV.dial CTV @14:54:49.97
SS.seek 10ml @14:54:51.07 CSV.dial :air @14:54:51.51 CS.reconfigure :fast @14:54:52.00 CS.seek 0ml @14:54:52.71
SSV.dial :spit,avoiding: :exhaust @14:54:54.03 SS.reconfigure :exhale @14:54:54.08 SS.seek 0ml @14:54:59.41 SS.reconfigure :fast @14:54:59.45 SS.seek 5ml @14:54:59.94 CS.stop @14:55:02.29 SSV.dial CBV,avoiding: :exhaust @14:55:02.32
CBV.dial SSV @14:55:04.67 SSV.dial :spit,avoiding: :exhaust @14:55:05.98 SS.reconfigure :default @14:55:06.03 SS.seek 0ml @14:55:09.32 SS.stop @14:55:09.36 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @14:55:09.37 Intake.select :close @14:55:09.43 CSV.dial :waste @14:55:09.57 SSV.dial CBV,avoiding: :exhaust @14:55:09.59
CBV.dial SSV @14:55:10.20 CS.seek 0ml @14:55:10.41 CSV.dial :air @14:55:10.95
CTV.dial :air @14:55:11.12 delay 15 seconds @14:55:11.16 CS.seek 10ml @14:55:26.14
SSV.dial :spit,avoiding: :exhaust @14:55:27.45 CTV.dial CSV,avoiding: :intake @14:55:27.50
SS.reconfigure :exhale @14:55:27.58 SS.seek 0ml @14:55:27.98 SSV.dial CBV,avoiding: :exhaust @14:55:35.51 SSV.dial between CBV and plug @14:55:35.99 CBV.dial between SSV and waste2 @14:55:36.38 CSV.dial between air and CTV @14:55:36.69 CS.seek 8.233ml @14:55:41.51 CTV.dial CSV,avoiding: :air @14:55:41.54
CSV.dial CTV @14:55:41.90 CS.reconfigure :pressurize @14:55:41.95 CS.seek 0ml @14:55:58.89 BubblePoint: Intake @24.1psia, Outlet @1.3psia @14:55:58.90 CBV.dial SSV @14:55:59.23 SS.reconfigure :default @14:55:59.28 SS.seek 10ml @14:56:04.91 delay 15 seconds @14:56:20.04 Evacuated: Intake @23.7psia, Outlet @16.1psia @14:56:20.05 SSV.dial :spit,avoiding: :exhaust @14:56:21.07 SS.reconfigure :exhale @14:56:21.12 SS.seek 0ml @14:56:26.11 SS.reconfigure :fast @14:56:26.16 SS.seek 5ml @14:56:29.20 SSV.dial CBV,avoiding: :exhaust @14:56:29.23
CBV.dial SSV @14:56:30.54 CTV.dial :air,avoiding: :intake @14:56:31.91 CSV.dial CTV @14:56:31.94
SS.seek 10ml @14:56:33.14 CSV.dial :air @14:56:33.57 CS.reconfigure :fast @14:56:33.62 CS.seek 0ml @14:56:34.57
SSV.dial :spit,avoiding: :exhaust @14:56:35.89 SS.reconfigure :exhale @14:56:35.93 SS.seek 0ml @14:56:38.60 CS.stop @14:56:41.31
SS.reconfigure :fast @14:56:41.36 SS.seek 5ml @14:56:44.23 SSV.dial CBV,avoiding: :exhaust @14:56:44.26
CBV.dial SSV @14:56:46.57 SSV.dial :spit,avoiding: :exhaust @14:56:47.87 SS.reconfigure :default @14:56:47.92 SS.seek 0ml @14:56:51.22 SS.stop @14:56:51.26 Lysing: Adding 2.0ml AqMeOH @14:56:51.28 CBV.dial between CSV and waste1,avoiding: :DBV @14:56:52.73 CTV.dial CSV @14:56:54.02 CSV.dial CSR @14:56:54.92 Pulling 1ml of CSR.AqMeOH @14:56:54.93 CSR.select :AqMeOH @14:56:55.05 CS.reconfigure :default @14:56:55.09 CS.seek 1ml @14:56:58.54 delay 5 seconds @14:57:03.56 CSR.select :air @14:57:03.72 CS.seek 6ml @14:57:17.38 delay 2 seconds @14:57:19.40 CSV.dial :waste2 @14:57:19.86 CS.reconfigure :fast @14:57:19.90 CS.seek 0ml @14:57:34.19 CSV.dial CSR @14:57:34.65 Pulling 2ml of CSR.AqMeOH CSR.select :AqMeOH @14:57:34.77 CS.reconfigure :default @14:57:34.82 CS.seek 2ml @14:57:40.72 delay 5 seconds @14:57:45.74 CSR.select :air @14:57:45.91 CS.seek 5ml @14:57:54.38 delay 2 seconds @14:57:56.40 CSV.dial CTV @14:57:56.84 CS.seek 1ml @14:58:07.96 Before lysing Collection Puck Heater at 21.7C/25.2psia @14:58:07.98 Heating Puck.from 2, :sh1 in Collection Clamp to 70C for 10:00 @14:58:07.99 CH.seek 70C,8:00 @14:59:56.61 CH.hold 10:00,70C @14:59:56.80 Begun lysing Collection Puck Heater at 68C/30.7psia @15:09:56.81 Ended lysing Collection Puck Heater at 70C/29.6psia @15:09:56.82 CS.seek 0ml @15:10:00.40 CTV.dial between spare and air,avoiding: :intake @15:10:01.41 CSV.dial :air @15:10:01.87 CS.seek 5ml @15:10:15.43 CSV.dial CTV @15:10:15.88 delay 2 seconds @15:10:17.90 CSV.dial :air @15:10:18.35 delay 2 seconds @15:10:20.37 CSV.dial CTV @15:10:20.83 delay 2 seconds @15:10:22.84 CSV.dial :air @15:10:23.29 delay 2 seconds @15:10:25.31 CS.seek 0ml @15:10:38.95 Puck @58.6psia after final lysis pressurization @15:10:38.96 Recovering Lysate into Collection syringe @15:10:38.97 CSV.dial CBV @15:10:39.98 CBV.dial CSV @15:10:40.35 CS.seek 5ml @15:10:54.02 delay 5 seconds @15:10:59.04 CTV.dial :air @15:10:59.55 CS.seek 7ml @15:11:05.87 delay 5 seconds @15:11:10.89 CSV.dial :air2 @15:11:11.33 CS.seek 10ml @15:11:19.78 CSV.dial between air and CTV,avoiding: CBV @15:11:20.92 Lysing Complete @15:11:20.99 Storing sh1 puck in Collection Clamp to tube 1 @15:11:21.16 SC.reconfigure :tight @15:11:21.21 SC.seek 1 @15:11:24.83 SC.configure :limp @15:11:24.98 SE.reconfigure :place @15:11:25.06
Elbow.reconfigure :tight @15:11:25.10 SE.seek :beamBreak @15:11:25.13
Elbow.seek CC @15:11:25.45 Elbow.reconfigure :loose @15:11:25.55 Forearm.seek CC @15:11:26.48 Hand.close @15:11:27.11 CC.seek :open @15:11:27.96 SE.configure :default @15:11:28.02 SE.seek :place @15:11:31.85
Forearm.seek :retract @15:11:32.80 Elbow.reconfigure :tight @15:11:32.85 Elbow.seek 1 @15:11:34.57 Elbow.reconfigure :limp @15:11:34.62 Forearm.seek 1 @15:11:35.59 Elbow.hold @15:11:35.66 Elbow.reconfigure :tight @15:11:35.71 Hand.open @15:11:36.48 Forearm.force -20 @15:11:36.52 Forearm.seek 1 + 314 counts @15:11:36.81 Forearm.reconfigure :tight @15:11:36.86 Forearm.seek 1 + 450 counts @15:11:37.11 SE.seek :down @15:11:38.24
Forearm.reconfigure :loose @15:11:38.68 Forearm.seek :retract @15:11:39.82 SE.stop @15:11:39.86
Tranferring DA @15:11:39.88 Beginning samplePassC @15:11:39.89 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @15:11:40.08 Elbow.seek FlushPuck::Garage @15:11:41.86 Elbow.reconfigure :loose @15:11:41.96 Forearm.seek :garage @15:11:42.92 Hand.close @15:11:43.60 Forearm.seek :retract @15:11:44.61 Elbow.reconfigure :tight @15:11:44.66 Elbow.seek CC @15:11:47.59 Elbow.reconfigure :loose @15:11:47.69 Forearm.reconfigure :tight @15:11:47.74 Forearm.seek CC @15:11:48.94 CC.seek :closed @15:11:53.47 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.31 @15:11:53.53 Hand.open @15:11:54.17 Forearm.reconfigure :loose @15:11:54.22 Forearm.seek :retract @15:11:55.23 PSV.dial PSR @15:11:56.62 Pulling 1.5ml of PSR.flush @15:11:56.63 PSR.select :flush @15:11:56.75 PS.seek 1.5ml @15:12:12.85 delay 5 seconds @15:12:17.87 PSV.dial :waste1 @15:12:18.32 PS.seek 0ml @15:12:34.44 PSV.dial PSR @15:12:34.91 PSR.select :air @15:12:35.03 PS.seek 2.5ml @15:13:01.80 PRV.dial CBV,avoiding: :ab2 @15:13:01.83
CBV.dial PRV @15:13:03.55 PSV.dial :mixloop @15:13:04.58 CTV.dial :air,avoiding: :intake @15:13:04.66 PTV.dial :PRVmixing @15:13:05.98 PS.seek 0ml @15:13:32.34 PSV.dial :air @15:13:32.80 PS.seek 2.5ml @15:13:59.22 PSV.dial :mixloop @15:13:59.69 PS.seek 0ml @15:14:26.03 PSV.dial :air @15:14:26.51 PS.seek 2.5ml @15:14:52.90 PSV.dial :mixloop @15:14:53.38 PS.seek 0ml @15:15:19.75 Garaging FlushPuck in Collection Clamp @15:15:19.82 Elbow.reconfigure :tight @15:15:19.87 Elbow.seek CC @15:15:20.15 Elbow.reconfigure :loose @15:15:20.25 Forearm.seek CC @15:15:21.26 Hand.close @15:15:21.86 CC.seek :open @15:15:26.51 Forearm.seek :retract @15:15:27.50 Elbow.reconfigure :tight @15:15:27.55 Elbow.seek :garage @15:15:30.42 Elbow.reconfigure :loose @15:15:30.53 Forearm.reconfigure :tight @15:15:30.58 Forearm.seek :garage @15:15:31.60 Hand.open @15:15:32.29 Forearm.reconfigure :loose @15:15:32.34 Forearm.seek :retract @15:15:33.37 PSV.dial :mixloop @15:15:33.44 PTV.dial :PRVmixing @15:15:33.52 PRV.dial CBV @15:15:33.54
CBV.dial PRV @15:15:33.74 CTV.dial :air,avoiding: :intake @15:15:33.82 Loading lyfil puck from tube 2 into Collection Clamp @15:15:34.42 SC.reconfigure :tight @15:15:34.54 SC.seek 2 @15:15:38.10 SC.configure :limp @15:15:38.31 Elbow.reconfigure :tight @15:15:38.36 Elbow.seek 2 @15:15:40.26 Forearm.reconfigure :tight @15:15:40.30 Forearm.seek 2 + 450 counts @15:15:41.34 SE.reconfigure :pick @15:15:41.40 SE.seek :beamBreak @15:15:44.34 SE.configure :default @15:15:44.50 Elbow.seek 2 @15:15:44.69 Forearm.seek 2 + 300 counts @15:15:44.92 Forearm.reconfigure :loose @15:15:44.96 Hand.close @15:15:45.62 SE.seek :down @15:15:45.81
Forearm.seek :retract @15:15:46.76 Elbow.seek CC @15:15:48.28 SE.stop @15:15:48.49
Elbow.reconfigure :loose @15:15:48.59 Forearm.reconfigure :tight @15:15:48.65 Forearm.seek CC @15:15:49.99 CC.seek :closed @15:15:54.81 Collection Clamp onSpring/offSpring for 13/27 samples, on/off torque 2.08 @15:15:54.82 Hand.open @15:15:55.55 Forearm.reconfigure :loose @15:15:55.59 Forearm.seek :retract @15:15:56.58 CTV.dial CSV,avoiding: :intake @15:15:57.92 CSV.dial CTV @15:15:58.37 PS.seek 2.5ml @15:16:24.78 CS.seek 4.499ml @15:16:39.61 delay 8 seconds @15:16:47.63 PSV.dial :air @15:16:48.09 delay 2 seconds @15:16:50.11 PSV.dial :waste2 @15:16:50.85 PS.seek 0ml @15:17:17.22 CBV.dial between PRV and waste1 @15:17:17.53 CSV.dial :air @15:17:17.98 CS.seek 10ml @15:17:32.83 CSV.dial CTV @15:17:33.29 CS.seek 0ml @15:17:59.67 CBV.dial :waste1 @15:17:59.99 delay 5 seconds @15:18:05.01 CTV.dial :air,avoiding: :intake @15:18:06.39 Storing lyfil puck in Collection Clamp to tube 1 @15:18:06.57 SC.reconfigure :tight @15:18:06.62 SC.seek 1 @15:18:10.32 SC.configure :limp @15:18:10.54 Elbow.reconfigure :tight @15:18:10.59 Elbow.seek 1 @15:18:12.35 Forearm.reconfigure :tight @15:18:12.40 Forearm.seek 1 + 450 counts @15:18:13.43 SE.reconfigure :pick @15:18:13.49 SE.seek :beamBreak @15:18:16.54 SE.configure :default @15:18:16.70 Elbow.reconfigure :limp @15:18:17.12 Forearm.reconfigure :loose @15:18:17.23 Forearm.seek 1 @15:18:17.45 Elbow.hold @15:18:17.51 Elbow.reconfigure :tight @15:18:17.62 SE.seek 0.16 Pucks below Top Plate @15:18:18.04 Forearm.force -20 @15:18:18.08 Forearm.seek 1 + 319 counts @15:18:18.37 Forearm.reconfigure :tight @15:18:18.41 Forearm.seek 1 + 450 counts @15:18:18.61 SE.seek 1.16 Pucks below Top Plate @15:18:19.11 Forearm.reconfigure :loose @15:18:19.16 Forearm.seek :retract @15:18:20.14 Elbow.seek CC @15:18:21.81 Elbow.reconfigure :loose @15:18:21.91 Forearm.seek CC @15:18:22.88 Hand.close @15:18:23.48 CC.seek :open @15:18:28.22 Forearm.seek :retract @15:18:29.21 Elbow.reconfigure :tight @15:18:29.26 Elbow.seek 1 @15:18:31.05 Elbow.reconfigure :limp @15:18:31.10 Forearm.seek 1 @15:18:32.07 Elbow.hold @15:18:32.13 Elbow.reconfigure :tight @15:18:32.18 Hand.open @15:18:32.98 Forearm.force -20 @15:18:33.03 Forearm.seek 1 + 319 counts @15:18:33.76 Forearm.reconfigure :tight @15:18:33.81 Forearm.seek 1 + 450 counts @15:18:34.06 SE.seek :down @15:18:35.19
Forearm.reconfigure :loose @15:18:35.24 Forearm.seek :retract @15:18:36.67 SE.stop @15:18:36.71
PSV.dial :air @15:18:37.47 PS.seek 2.5ml @15:19:03.84 delay 1 second @15:19:04.85 PSV.dial :mixloop @15:19:05.29 delay 1 second @15:19:06.31 PSV.dial :air @15:19:06.89 delay 1 second @15:19:07.90 PSV.dial :mixloop @15:19:08.36 delay 1 second @15:19:09.37 PSV.dial :air @15:19:09.84 delay 1 second @15:19:10.86 PS.seek 0ml @15:19:37.25 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @15:19:37.43 Elbow.seek FlushPuck::Garage @15:19:39.27 Elbow.reconfigure :loose @15:19:39.37 Forearm.seek :garage @15:19:40.38 Hand.close @15:19:41.09 Forearm.seek :retract @15:19:42.07 Elbow.reconfigure :tight @15:19:42.12 Elbow.seek PC @15:19:43.38 Elbow.reconfigure :loose @15:19:43.48 Forearm.reconfigure :tight @15:19:43.52 Forearm.seek PC @15:19:44.74 PC.seek :closed @15:19:49.38 Processing Clamp onSpring/offSpring for 16/26 samples, on/off torque 1.55 @15:19:49.46 Hand.open @15:19:50.16 Forearm.reconfigure :loose @15:19:50.21 Forearm.seek :retract @15:19:51.22 PRV.dial :air @15:19:51.69 PSV.dial :mixloop @15:19:52.21 PS.seek 0.3ml @15:19:56.12 PSV.dial PSR @15:19:57.17 Pulling 0.4ml of PSR.air PSR.select :air @15:19:57.20 PS.seek 0.7ml @15:20:02.18 delay 3 seconds @15:20:05.20 PSR.select :air @15:20:05.22 PS.seek 2.5ml @15:20:24.46 delay 3 seconds @15:20:27.48 PSV.dial :mixloop @15:20:28.65 PTV.dial :mixing @15:20:29.41 PBV.dial :waste2 @15:20:30.36 PS.seek 0ml @15:20:56.74 Garaging FlushPuck in Processing Clamp @15:20:56.82 Elbow.reconfigure :tight @15:20:56.87 Elbow.seek PC @15:20:56.99 Elbow.reconfigure :loose @15:20:57.09 Forearm.seek PC @15:20:58.10 Hand.close @15:20:58.73 PC.seek :open @15:21:03.46 Forearm.seek :retract @15:21:04.42 Elbow.reconfigure :tight @15:21:04.46 Elbow.seek :garage @15:21:05.86 Elbow.reconfigure :loose @15:21:05.96 Forearm.reconfigure :tight @15:21:06.00 Forearm.seek :garage @15:21:07.08 Hand.open @15:21:07.79 Forearm.reconfigure :loose @15:21:08.25 Forearm.seek :retract @15:21:09.23 Loading da puck from tube 2 into Processing Clamp @15:21:09.47 SC.reconfigure :tight @15:21:09.52 SC.seek 2 @15:21:13.11 SC.configure :limp @15:21:13.32 Elbow.reconfigure :tight @15:21:13.37 Elbow.seek 2 @15:21:15.22 Forearm.reconfigure :tight @15:21:15.27 Forearm.seek 2 + 450 counts @15:21:16.48 SE.reconfigure :pick @15:21:16.53 SE.seek :beamBreak @15:21:19.71 SE.configure :default @15:21:19.87 Elbow.seek 2 @15:21:20.18 Forearm.seek 2 + 300 counts @15:21:20.46 Forearm.reconfigure :loose @15:21:20.50 Hand.close @15:21:21.11 SE.seek :down @15:21:21.32
Forearm.seek :retract @15:21:22.32 Elbow.seek PC @15:21:23.83 SE.stop @15:21:24.82
Elbow.reconfigure :loose @15:21:24.92 Forearm.reconfigure :tight @15:21:24.97 Forearm.seek PC @15:21:26.29 PC.seek :closed @15:21:30.96 Processing Clamp onSpring/offSpring for 16/26 samples, on/off torque 1.59 @15:21:30.97 Hand.open @15:21:31.67 Forearm.reconfigure :loose @15:21:31.72 Forearm.seek :retract @15:21:32.62 Heating Puck.from 2, :da in Processing Clamp to 28C for 2:00:00 @15:21:32.64 PH.seek 28C,5:00 @15:22:02.25 PH.hold 2:00:00,28C @15:22:02.31 delay 1:30 @15:23:32.35 PSR.select :air @15:23:32.37 PSV.dial PSR @15:23:33.40 Pulling 0.5ml of PSR.wash @15:23:33.41 PSR.select :wash @15:23:33.53 PS.seek 0.5ml @15:23:39.52 delay 5 seconds @15:23:44.54 PSR.select :air @15:23:44.66 PS.seek 2ml @15:24:00.77 PTV.dial :puck @15:24:01.50 PSV.dial PTV @15:24:02.25 PBV.dial :waste1 @15:24:03.63 PS.seek 1.5ml @15:24:09.65 delay 3 seconds @15:24:12.66 PBV.dial between PSV and waste1 @15:24:13.03 PS.seek 0ml @15:24:29.59 delay 5:00 @15:29:29.61 recoverPSRreagent08 @15:29:29.62 PTV.dial :puck @15:29:29.70 PBV.dial :waste1 @15:29:30.08 delay 30 seconds @15:30:00.10 PBV.dial between PSV and waste1 @15:30:00.48 PSR.select :air @15:30:00.50 PSV.dial PSR @15:30:01.25 PS.reconfigure :fast @15:30:01.30 PS.seek 2.5ml @15:30:25.01 PSV.dial PTV @15:30:25.89 delay 5 seconds @15:30:30.91 PSV.dial PSR @15:30:31.64 delay 5 seconds @15:30:36.66 PSV.dial PTV @15:30:37.42 delay 5 seconds @15:30:42.44 PSV.dial :air @15:30:43.21 PS.seek 0ml @15:31:06.42 PTV.dial :PRVmixing,via: PRV @15:31:07.73 PSV.dial :mixloop @15:31:08.20 PRV.dial :ab1 @15:31:08.94 PS.reconfigure :slow @15:31:08.99 PS.seek 0.15ml @15:31:12.61 delay 2 seconds @15:31:14.63 CBV.dial PRV @15:31:14.65
PRV.dial CBV @15:31:15.21 PS.seek 0.34ml @15:31:19.61 delay 2 seconds @15:31:21.62 CBV.dial between PRV and PSV @15:31:21.94 PRV.dial :air @15:31:22.39 PS.seek 0.9ml @15:31:34.35 PRV.dial :waste2 @15:31:35.10 PS.seek 0.5ml @15:31:43.73 addPMLReagent08 @15:31:43.74 PSV.dial :air @15:31:44.31 PS.reconfigure :default @15:31:44.36 PS.seek 2ml @15:32:00.48 PSV.dial :PML @15:32:00.93 PTV.dial :mixing @15:32:01.67 PBV.dial :waste1 @15:32:02.11 PS.seek 1.85ml @15:32:04.48 PBV.dial between PSV and waste1 @15:32:04.86 PS.seek 0ml @15:32:24.64 delay 2 seconds @15:32:26.65 Beginning samplePassC @15:32:26.67 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @15:32:26.85 Elbow.reconfigure :tight @15:32:26.90 Elbow.seek FlushPuck::Garage @15:32:28.69 Elbow.reconfigure :loose @15:32:28.79 Forearm.seek :garage @15:32:29.80 Hand.close @15:32:30.59 Forearm.seek :retract @15:32:31.58 Elbow.reconfigure :tight @15:32:31.63 Elbow.seek CC @15:32:34.51 Elbow.reconfigure :loose @15:32:34.61 Forearm.reconfigure :tight @15:32:34.66 Forearm.seek CC @15:32:35.93 CC.seek :closed @15:32:40.45 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.35 @15:32:40.51 Hand.open @15:32:41.16 Forearm.reconfigure :loose @15:32:41.21 Forearm.seek :retract @15:32:42.18 PSV.dial PSR @15:32:43.35 Pulling 1.5ml of PSR.flush @15:32:43.36 PSR.select :flush @15:32:43.49 PS.seek 1.5ml @15:32:59.57 delay 5 seconds @15:33:04.59 PSV.dial :waste1 @15:33:05.08 PS.seek 0ml @15:33:21.16 PSV.dial PSR @15:33:21.63 PSR.select :air @15:33:21.75 PS.seek 2.5ml @15:33:48.08 PRV.dial CBV,avoiding: :ab2 @15:33:48.10
CBV.dial PRV @15:33:49.73 PSV.dial :mixloop @15:33:50.76 CTV.dial :air,avoiding: :intake @15:33:51.28 PTV.dial :PRVmixing @15:33:52.03 PS.seek 0ml @15:34:18.57 PSV.dial :air @15:34:19.15 PS.seek 2.5ml @15:34:45.48 PSV.dial :mixloop @15:34:45.93 PS.seek 0ml @15:35:12.26 PSV.dial :air @15:35:12.73 PS.seek 2.5ml @15:35:39.07 PSV.dial :mixloop @15:35:39.67 PS.seek 0ml @15:36:06.01 Garaging FlushPuck in Collection Clamp @15:36:06.08 Elbow.reconfigure :tight @15:36:06.13 Elbow.seek CC @15:36:06.39 Elbow.reconfigure :loose @15:36:06.50 Forearm.seek CC @15:36:07.52 Hand.close @15:36:08.09 CC.seek :open @15:36:12.74 Forearm.seek :retract @15:36:13.73 Elbow.reconfigure :tight @15:36:13.78 Elbow.seek :garage @15:36:16.68 Elbow.reconfigure :loose @15:36:16.78 Forearm.reconfigure :tight @15:36:16.82 Forearm.seek :garage @15:36:17.76 Hand.open @15:36:18.40 Forearm.reconfigure :loose @15:36:18.45 Forearm.seek :retract @15:36:19.42 delayUntil "15:40:26.64" @15:36:19.45 Whole Cell Archive sampling 50ml @15:36:19.46 Loading wcr puck from tube 2 into Collection Clamp @15:36:19.71 SC.reconfigure :tight @15:36:19.76 SC.seek 2 @15:36:19.91 SC.configure :limp @15:36:20.57 Elbow.reconfigure :tight @15:36:20.63 Elbow.seek 2 @15:36:22.62 Forearm.reconfigure :tight @15:36:22.67 Forearm.seek 2 + 450 counts @15:36:23.72 SE.reconfigure :pick @15:36:23.78 SE.seek :beamBreak @15:36:26.82 SE.configure :default @15:36:26.98 Elbow.seek 2 @15:36:27.17 Forearm.seek 2 + 300 counts @15:36:27.40 Forearm.reconfigure :loose @15:36:27.45 Hand.close @15:36:28.10 Puck::Warning in WCR -- Loaded Last remaining Puck (from Tube 2) @15:36:28.12 SE.seek :down @15:36:28.35 Forearm.seek :retract @15:36:29.31 Elbow.seek CC @15:36:30.93 SE.stop @15:36:30.96 Elbow.reconfigure :loose @15:36:31.10 Forearm.reconfigure :tight @15:36:31.16 Forearm.seek CC @15:36:32.50 CC.seek :closed @15:36:37.04 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.24 @15:36:37.05 Hand.open @15:36:38.15 Forearm.reconfigure :loose @15:36:38.20 Forearm.seek :retract @15:36:39.20 collectSample 50ml w/bubble point of 35psi @15:36:39.21 Priming 7ml @15:36:39.22 Intake.select :close @15:36:39.24 Exhaust.select :close @15:36:39.25 SS.hold @15:36:39.47 Calibrating pressure sensors @15:36:39.48 CTV.dial :air @15:36:39.56 SSV.dial :spit,avoiding: :exhaust @15:36:39.65 SS.reconfigure :fast @15:36:39.70 SS.seek 5ml @15:36:42.35 SSV.dial CBV @15:36:42.38 CBV.dial SSV @15:36:43.75 delay 3.8 seconds @15:36:47.69 Sensor limits: Intake max 112.0psia; Outlet max 105.7psia @15:36:47.70 SSV.dial :spit @15:36:49.04 SS.seek 0ml @15:36:51.95 SSV.dial CBV @15:36:53.29 CTV.dial :intake @15:36:54.03 SSV.dial CBV @15:36:54.06 CBV.dial SSV @15:36:54.30 Allowing 40s for 7.0ml of remaining 7.0ml @15:36:54.31 Intake.select :open @15:36:56.48 Intake @15.3psia, vacuum from 9.8 to 12.8psi @15:36:56.50 SS.reconfigure :inhaling 2.20ml/s @15:36:56.55 SS.seek 7ml @15:37:02.00 Stroke completed -- waiting for pressure to equalize ... @15:37:02.01 SS.reconfigure :exhale @15:37:02.06 SS.hold @15:37:06.13 Intake.select :close @15:37:06.29 SSV.dial :exhaust @15:37:07.07 Exhaust.select :open @15:37:07.19 SS.seek 0ml @15:37:11.20 Exhaust.select :close @15:37:11.32 Sampling 50.0ml @15:37:11.33 Intake.select :close @15:37:11.34 Exhaust.select :close @15:37:11.36 SS.reconfigure :default @15:37:11.40 SS.hold @15:37:11.62 CTV.dial :intake @15:37:11.70 SSV.dial CBV @15:37:11.73 CBV.dial SSV @15:37:12.52 Allowing 130s for 25.0ml of remaining 50.0ml @15:37:12.53 Intake.select :open @15:37:14.70 Intake @15.8psia, vacuum from 10.0 to 13.0psi @15:37:14.72 SS.reconfigure :inhaling 2.20ml/s @15:37:14.77 SS.seek 25ml @15:37:29.81 Stroke completed -- waiting for pressure to equalize ... @15:37:29.82 SS.reconfigure :exhale @15:37:29.87 SS.hold @15:37:32.85 Intake.select :close @15:37:33.01 SSV.dial :exhaust @15:37:33.78 Exhaust.select :open @15:37:33.90 SS.seek 0ml @15:37:42.67 Exhaust.select :close @15:37:42.79 SSV.dial CBV @15:37:42.82 CBV.dial SSV @15:37:43.59 Allowing 130s for 25.0ml of remaining 25.0ml @15:37:43.61 Intake.select :open @15:37:46.21 Intake @15.8psia, vacuum from 10.0 to 13.0psi @15:37:46.23 SS.reconfigure :inhaling 2.20ml/s @15:37:46.28 SS.seek 25ml @15:38:01.54 Stroke completed -- waiting for pressure to equalize ... @15:38:01.56 SS.reconfigure :exhale @15:38:01.61 SS.hold @15:38:04.58 Intake.select :close @15:38:04.74 SSV.dial :exhaust @15:38:05.50 Exhaust.select :open @15:38:05.62 SS.seek 0ml @15:38:14.47 Exhaust.select :close @15:38:14.58 Sampled 50.0ml @15:38:14.59 Drying puck before fixing for 5:00 @15:38:14.60 CBV.dial :waste2b @15:38:15.36 delay 1:00 @15:39:15.38 CSV.dial :air @15:39:15.84 CS.reconfigure :fast @15:39:15.90 CS.seek 10ml @15:39:40.53 Pumping wcr puck from tube 2 up to 35psi... @15:39:40.54 CBV.dial SSV @15:39:41.81 CBV.dial between CSV and waste2b @15:39:42.79 CSV.dial between air and CTV @15:39:43.11 CS.seek 7.272ml @15:39:50.19 CTV.dial CSV,avoiding: :air @15:39:50.22 CSV.dial CTV @15:39:51.02 CS.reconfigure :pressurize @15:39:51.07 CS.seek 0ml @15:40:04.58 CBV.dial :waste2b @15:40:04.90 CS.reconfigure :default @15:40:05.40 CS.seek 0ml @15:40:11.81 delay 1:00 @15:40:26.66 recoverPRVreagent08 @15:40:26.67 PTV.dial :mixing @15:40:27.42 PBV.dial :waste1 @15:40:27.80 delay 30 seconds @15:40:57.82 PBV.dial between PSV and waste1 @15:40:58.19 PSV.dial :air @15:40:58.65 PS.reconfigure :fast @15:40:58.70 PS.seek 2.5ml @15:41:11.83 CTV.dial :air,avoiding: :intake @15:41:13.14 Fix #1 of 2 (with RNAlater) for 2:30 @15:41:13.16 CSV.dial CSR @15:41:13.59 Pulling 2ml of CSR.RNAlater @15:41:13.60 CSR.select :RNAlater @15:41:13.72 CS.seek 2ml @15:41:19.71 delay 5 seconds @15:41:22.78 PSV.dial :mixloop @15:41:23.25 delay 2 seconds @15:41:24.73 CSR.select :air @15:41:24.85 CS.reconfigure :fast @15:41:24.90 CS.seek 10ml @15:41:25.27 PSV.dial :air @15:41:25.73 delay 2 seconds @15:41:27.75 PSV.dial :mixloop @15:41:28.22 delay 2 seconds @15:41:30.24 PSV.dial :air @15:41:30.71 PS.seek 0ml @15:41:44.59 Pumping wcr puck from tube 2 up to 35psi... @15:41:44.61 CBV.dial SSV @15:41:45.86 CBV.dial between CSV and waste2b @15:41:46.82 CSV.dial between air and CTV @15:41:47.42 CS.seek 7.289ml @15:41:54.35 washCycleDA #1 of 2 @15:41:54.36 PSR.select :air @15:41:54.37 PSV.dial PSR @15:41:54.44 CTV.dial CSV,avoiding: :air @15:41:54.48 CSV.dial CTV @15:41:55.70 Pulling 0.5ml of PSR.wash @15:41:55.71 PSR.select :wash @15:41:55.84 CS.reconfigure :pressurize @15:41:55.90 CS.seek 0ml @15:41:55.95 PS.reconfigure :default @15:41:56.00 PS.seek 0.5ml @15:42:01.94 delay 5 seconds @15:42:06.96 PSR.select :air @15:42:07.08 PS.seek 2ml @15:42:15.12 CSV.dial :air @15:42:15.58 CS.reconfigure :fast @15:42:16.07 CS.seek 10ml @15:42:23.18 PTV.dial :puck @15:42:23.92 PSV.dial PTV @15:42:24.67 PBV.dial :waste1 @15:42:25.10 PS.seek 1.5ml @15:42:31.19 delay 3 seconds @15:42:34.21 PBV.dial between PSV and waste1 @15:42:34.58 PS.seek 0ml @15:42:40.61 CSV.dial between air and CTV @15:42:40.94 CS.seek 4.688ml @15:42:50.69 delay 2:00 @15:42:53.87 CTV.dial CSV,avoiding: :air @15:42:53.90 CSV.dial CTV @15:42:54.25 CS.reconfigure :pressurize @15:42:54.30 CS.seek 0ml @15:43:06.77 CSV.dial :air @15:43:07.23 CS.reconfigure :fast @15:43:07.28 CS.seek 10ml @15:43:32.05 CSV.dial between air and CTV @15:43:32.37 CS.seek 4.864ml @15:43:44.87 CTV.dial CSV,avoiding: :air @15:43:44.90 CSV.dial CTV @15:43:45.26 CS.reconfigure :pressurize @15:43:45.31 CS.seek 0ml @15:43:58.27 CSV.dial :air @15:43:58.73 CS.reconfigure :fast @15:43:58.78 CS.seek 10ml @15:44:23.46 CSV.dial between air and CTV @15:44:23.78 CS.seek 4.989ml @15:44:36.03 CTV.dial CSV,avoiding: :air @15:44:36.06 CSV.dial CTV @15:44:36.36 CS.reconfigure :pressurize @15:44:36.41 CS.seek 0ml @15:44:50.11 CSV.dial :air @15:44:50.57 CS.reconfigure :default @15:44:50.62 CS.hold @15:44:50.74 recoverPSRreagent08 @15:44:50.76 PTV.dial :puck @15:44:50.83 ShallowSampler::Error in MAIN -- Puck.from 2, :wcr reached only 14.1 of 35psi after pumping in 40ml of gas @15:44:50.85
Waiting to Resume from Checkpoint "Pumping wcr puck from tube 2 up to 35psi..." @15:44:50.87 BadNews.email "Puck.from 2, :wcr reached only 14.1 of 35psi after pumping in 40ml of gas",Subject: "ShallowSampler::Error in MAIN" @15:44:50.95 PBV.dial :waste1 @15:44:51.34 delay 30 seconds @15:44:55.11 upsync @15:45:21.37 PBV.dial between PSV and waste1 @15:45:21.75 PSR.select :air @15:45:21.77 PSV.dial PSR @15:45:22.55 PS.reconfigure :fast @15:45:22.60 PS.seek 2.5ml @15:45:46.53 PSV.dial PTV @15:45:47.29 delay 5 seconds @15:45:52.32 PSV.dial PSR @15:45:53.07 delay 5 seconds @15:45:58.10 PSV.dial PTV @15:45:58.96 delay 5 seconds @15:46:03.99 PSV.dial :air @15:46:04.88 PS.seek 0ml @15:46:28.31 washCycleDA #2 of 2 @15:46:28.32 PSR.select :air @15:46:28.34 PSV.dial PSR @15:46:29.67 Pulling 0.5ml of PSR.wash @15:46:29.68 PSR.select :wash @15:46:29.80 PS.reconfigure :default @15:46:29.85 PS.seek 0.5ml @15:46:35.74 delay 5 seconds @15:46:40.76 PSR.select :air @15:46:40.89 PS.seek 2ml @15:46:57.09 PTV.dial :puck @15:46:57.16 PSV.dial PTV @15:46:57.93 PBV.dial :waste1 @15:46:58.37 PS.seek 1.5ml @15:47:04.27 delay 3 seconds @15:47:07.29 PBV.dial between PSV and waste1 @15:47:07.67 PS.seek 0ml @15:47:24.00 delay 2:00 @15:49:24.03 recoverPSRreagent08 @15:49:24.04 PTV.dial :puck @15:49:24.12 PBV.dial :waste1 @15:49:24.49 delay 30 seconds @15:49:54.52 PBV.dial between PSV and waste1 @15:49:54.90 PSR.select :air @15:49:54.92 PSV.dial PSR @15:49:55.67 PS.reconfigure :fast @15:49:55.72 PS.seek 2.5ml @15:50:19.68 PSV.dial PTV @15:50:20.90 delay 5 seconds @15:50:25.92 PSV.dial PSR @15:50:26.66 delay 5 seconds @15:50:31.68 PSV.dial PTV @15:50:32.46 delay 5 seconds @15:50:37.48 PSV.dial :air @15:50:38.23 PS.seek 0ml @15:51:01.61 getPMLReagent08 @15:51:01.63 PRV.dial :air @15:51:02.08 delay 5 seconds @15:51:07.10 PTV.dial :PRVmixing @15:51:08.39 delay 5 seconds @15:51:13.42 PRV.dial :ab2 @15:51:13.86 PTV.dial :PRVmixing @15:51:13.94 PSV.dial :mixloop @15:51:14.41 PS.reconfigure :default @15:51:14.46 PS.seek 0.3ml @15:51:18.43 delay 2 seconds @15:51:20.45 PRV.dial :air @15:51:20.97 PS.seek 0.55ml @15:51:24.33 addPMLReagent08 @15:51:24.34 PSV.dial :air @15:51:24.82 PS.seek 2ml @15:51:40.52 PSV.dial :PML @15:51:40.99 PTV.dial :mixing @15:51:41.74 PBV.dial :waste1 @15:51:42.17 PS.seek 1.85ml @15:51:44.58 PBV.dial between PSV and waste1 @15:51:44.96 PS.seek 0ml @15:52:04.67 delay 2 seconds @15:52:06.69 delay 8:00 @16:00:06.72 recoverPRVreagent08 @16:00:06.73 PTV.dial :mixing @16:00:06.80 PBV.dial :waste1 @16:00:07.18 delay 30 seconds @16:00:37.21 PBV.dial between PSV and waste1 @16:00:37.58 PSV.dial :air @16:00:38.06 PS.reconfigure :fast @16:00:38.11 PS.seek 2.5ml @16:01:02.07 PSV.dial :mixloop @16:01:02.54 delay 2 seconds @16:01:04.56 PSV.dial :air @16:01:05.02 delay 2 seconds @16:01:07.04 PSV.dial :mixloop @16:01:07.51 delay 2 seconds @16:01:09.53 PSV.dial :air @16:01:10.00 PS.seek 0ml @16:01:33.26 washCycleDA #1 of 3 @16:01:33.27 PSR.select :air @16:01:33.29 PSV.dial PSR @16:01:34.60 Pulling 0.5ml of PSR.wash @16:01:34.61 PSR.select :wash @16:01:34.73 PS.reconfigure :default @16:01:34.78 PS.seek 0.5ml @16:01:40.79 delay 5 seconds @16:01:45.81 PSR.select :air @16:01:45.93 PS.seek 2ml @16:02:02.10 PTV.dial :puck @16:02:02.83 PSV.dial PTV @16:02:03.57 PBV.dial :waste1 @16:02:04.46 PS.seek 1.5ml @16:02:10.38 delay 3 seconds @16:02:13.40 PBV.dial between PSV and waste1 @16:02:13.78 PS.seek 0ml @16:02:29.88 delay 2:00 @16:04:29.91 recoverPSRreagent08 @16:04:29.92 PTV.dial :puck @16:04:30.10 PBV.dial :waste1 @16:04:30.47 delay 30 seconds @16:05:00.50 PBV.dial between PSV and waste1 @16:05:00.88 PSR.select :air @16:05:00.90 PSV.dial PSR @16:05:01.65 PS.reconfigure :fast @16:05:01.70 PS.seek 2.5ml @16:05:25.38 PSV.dial PTV @16:05:26.13 delay 5 seconds @16:05:31.15 PSV.dial PSR @16:05:31.89 delay 5 seconds @16:05:36.92 PSV.dial PTV @16:05:37.66 delay 5 seconds @16:05:42.68 PSV.dial :air @16:05:43.57 PS.seek 0ml @16:06:06.82 washCycleDA #2 of 3 @16:06:06.83 PSR.select :air @16:06:06.84 PSV.dial PSR @16:06:08.17 Pulling 0.5ml of PSR.wash @16:06:08.18 PSR.select :wash @16:06:08.30 PS.reconfigure :default @16:06:08.36 PS.seek 0.5ml @16:06:14.41 delay 5 seconds @16:06:19.43 PSR.select :air @16:06:19.55 PS.seek 2ml @16:06:35.65 PTV.dial :puck @16:06:35.83 PSV.dial PTV @16:06:36.59 PBV.dial :waste1 @16:06:37.03 PS.seek 1.5ml @16:06:43.06 delay 3 seconds @16:06:46.08 PBV.dial between PSV and waste1 @16:06:46.46 PS.seek 0ml @16:07:02.63 delay 2:00 @16:09:02.65 recoverPSRreagent08 @16:09:02.66 PTV.dial :puck @16:09:02.68 PBV.dial :waste1 @16:09:03.04 delay 30 seconds @16:09:33.07 PBV.dial between PSV and waste1 @16:09:33.44 PSR.select :air @16:09:33.45 PSV.dial PSR @16:09:34.33 PS.reconfigure :fast @16:09:34.38 PS.seek 2.5ml @16:09:58.12 PSV.dial PTV @16:09:58.86 delay 5 seconds @16:10:03.88 PSV.dial PSR @16:10:04.62 delay 5 seconds @16:10:09.64 PSV.dial PTV @16:10:10.40 delay 5 seconds @16:10:15.43 PSV.dial :air @16:10:16.17 PS.seek 0ml @16:10:39.47 washCycleDA #3 of 3 @16:10:39.48 PSR.select :air @16:10:39.49 PSV.dial PSR @16:10:40.81 Pulling 0.5ml of PSR.wash @16:10:40.82 PSR.select :wash @16:10:40.94 PS.reconfigure :default @16:10:40.98 PS.seek 0.5ml @16:10:47.37 delay 5 seconds @16:10:52.45 PSR.select :air @16:10:52.58 PS.seek 2ml @16:11:08.68 PTV.dial :puck @16:11:08.70 PSV.dial PTV @16:11:09.43 PBV.dial :waste1 @16:11:09.86 PS.seek 1.5ml @16:11:15.84 delay 3 seconds @16:11:18.86 PBV.dial between PSV and waste1 @16:11:19.23 PS.seek 0ml @16:11:35.37 delay 2:00 @16:13:35.40 recoverPSRreagent08 @16:13:35.41 PTV.dial :puck @16:13:35.43 PBV.dial :waste1 @16:13:35.81 delay 30 seconds @16:14:05.83 PBV.dial between PSV and waste1 @16:14:06.21 PSR.select :air @16:14:06.23 PSV.dial PSR @16:14:07.11 PS.reconfigure :fast @16:14:07.16 PS.seek 2.5ml @16:14:31.18 PSV.dial PTV @16:14:31.91 delay 5 seconds @16:14:36.94 PSV.dial PSR @16:14:37.68 delay 5 seconds @16:14:42.70 PSV.dial PTV @16:14:43.46 delay 5 seconds @16:14:48.48 PSV.dial :air @16:14:49.23 PS.seek 0ml @16:15:12.49 Gate.power :camera,:ON @16:15:12.50 Mixing DA Substrates @16:15:12.51 PSR.select :air @16:15:12.53 PSV.dial PSR @16:15:13.92 Pulling 0.25ml of PSR.sub1 @16:15:13.94 PSR.select :sub1 @16:15:14.19 PS.reconfigure :default @16:15:14.31 PS.seek 0.25ml @16:15:17.72 delay 5 seconds @16:15:22.74 Pulling 0.25ml of PSR.sub2 @16:15:22.75 PSR.select :sub2 @16:15:22.87 PS.seek 0.5ml @16:15:26.21 delay 5 seconds @16:15:31.23 PSR.select :air @16:15:31.34 PS.seek 2ml @16:15:47.46 PTV.dial :puck @16:15:47.47 PSV.dial PTV @16:15:48.35 PBV.dial :waste1 @16:15:48.78 PS.seek 1.5ml @16:15:54.68 delay 3 seconds @16:15:57.70 PBV.dial between PSV and waste1 @16:15:58.07 PS.seek 0ml @16:16:14.33 delay 10 seconds @16:16:24.36 recoverPSRreagent08 @16:16:24.37 PTV.dial :puck @16:16:24.38 PBV.dial :waste1 @16:16:24.76 delay 30 seconds @16:16:54.78 PBV.dial between PSV and waste1 @16:16:55.14 PSR.select :air @16:16:55.16 PSV.dial PSR @16:16:55.91 PS.reconfigure :fast @16:16:55.96 PS.seek 2.5ml @16:17:20.27 PSV.dial PTV @16:17:21.03 delay 5 seconds @16:17:26.06 PSV.dial PSR @16:17:26.80 delay 5 seconds @16:17:31.82 PSV.dial PTV @16:17:32.72 delay 5 seconds @16:17:37.74 PSV.dial :air @16:17:38.59 PS.seek 0ml @16:18:02.33 Additional air clear @16:18:02.34 PSR.select :air @16:18:02.36 PSV.dial PSR @16:18:03.71 Pulling 2ml of PSR.air @16:18:03.72 PSR.select :air @16:18:03.74 PS.reconfigure :default @16:18:03.79 PS.seek 2ml @16:18:25.11 delay 5 seconds @16:18:30.13 PTV.dial :puck @16:18:30.15 PSV.dial PTV @16:18:30.89 PBV.dial :waste1 @16:18:31.31 PS.seek 1.5ml @16:18:37.45 delay 3 seconds @16:18:40.47 PBV.dial between PSV and waste1 @16:18:40.85 delay 1 second @16:18:41.86 PSV.dial :waste1 @16:18:42.93 PS.seek 0ml @16:18:59.13 Processing Puck Heater at 28C before imaging @16:18:59.14 PH.coast @16:18:59.26 Imaging da puck in Processing Clamp while storing it to tube 1 @16:18:59.44 SC.reconfigure :tight @16:18:59.49 SC.seek 1 @16:19:03.25 SC.configure :limp @16:19:03.46 Elbow.reconfigure :tight @16:19:03.52 Elbow.seek 1 @16:19:05.30 Forearm.reconfigure :tight @16:19:05.35 Forearm.seek 1 + 450 counts @16:19:06.38 SE.reconfigure :pick @16:19:06.43 SE.seek :beamBreak @16:19:09.48 SE.configure :default @16:19:09.64 Elbow.reconfigure :limp @16:19:09.70 Forearm.reconfigure :loose @16:19:09.74 Forearm.seek 1 @16:19:10.02 Elbow.hold @16:19:10.08 Elbow.reconfigure :tight @16:19:10.19 SE.seek 1.16 Pucks below Top Plate @16:19:10.62 Forearm.force -20 @16:19:10.67 Forearm.seek 1 + 296 counts @16:19:10.95 Forearm.reconfigure :tight @16:19:11.00 Forearm.seek 1 + 450 counts @16:19:11.69 SE.seek 2.16 Pucks below Top Plate @16:19:12.24 Forearm.reconfigure :loose @16:19:12.29 Forearm.seek :retract @16:19:13.32 Elbow.seek PC @16:19:15.82 Elbow.reconfigure :loose @16:19:15.92 Forearm.seek PC @16:19:16.88 Hand.close @16:19:17.55 PC.seek :open @16:19:22.35 Forearm.seek :retract @16:19:23.29 Elbow.reconfigure :tight @16:19:23.34 Elbow.seek 1 @16:19:25.93 Forearm.reconfigure :tight @16:19:25.98 Forearm.seek 1 + 450 counts @16:19:26.92 Hand.open @16:19:27.62 SE.seek 1.81 Pucks below Top Plate @16:19:27.91 SE.reconfigure :seal @16:19:27.96 SE.seek 0.84 Pucks below Top Plate @16:19:28.55 Lights, camera, ACTION! @16:19:28.58 PuckCamera: snap -bin=2,2 -auto /var/log/esp/da24jan1616h50ml.tif @16:19:35.44 SX HX9/16 (USB:03): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 2.583 seconds /var/log/esp/da24jan1616h50ml.tif: TIFF Upload Complete @16:19:35.47 PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da24jan1616h50ml15s.tif @16:19:48.01 Waiting >20 seconds for da to relinquish FlushPuck @16:19:51.17 SX HX9/16 (USB:03): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15 seconds /var/log/esp/midres/da24jan1616h50ml15s.tif: TIFF Upload Complete @16:19:51.20 PuckCamera: snap -bin=2,2 20.0 /var/log/esp/midres/da24jan1616h50ml20s.tif @16:20:11.87 SX HX9/16 (USB:03): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 20 seconds /var/log/esp/midres/da24jan1616h50ml20s.tif: TIFF Upload Complete @16:20:11.89 Gate.power :camera,:OFF @16:20:11.91 DAfans.email "2.583 sec auto-exposure",Subject: "da24jan1616h50ml images!" @16:20:12.41 Elbow.seek 1 @16:20:12.64 Forearm.seek 1 + 450 counts @16:20:12.73 SE.reconfigure :gently @16:20:12.79 SE.seek 1.56 Pucks below Top Plate @16:20:13.42 Elbow.reconfigure :limp @16:20:13.47 Forearm.reconfigure :loose @16:20:13.54 Forearm.seek 1 @16:20:13.77 Elbow.hold @16:20:13.84 Elbow.reconfigure :tight @16:20:14.49 SE.reconfigure :default @16:20:14.54 SE.seek 1.74 Pucks below Top Plate @16:20:14.93 Forearm.force -20 @16:20:14.97 Forearm.seek 1 + 308 counts @16:20:15.28 Forearm.reconfigure :tight @16:20:15.41 Forearm.seek 1 + 450 counts @16:20:15.86 SE.seek :down @16:20:17.15 Forearm.reconfigure :loose @16:20:17.27 Forearm.seek :retract @16:20:18.60 SE.stop @16:20:18.82 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @16:20:19.23 Elbow.seek FlushPuck::Garage @16:20:21.27 Elbow.reconfigure :loose @16:20:21.49 Forearm.seek :garage @16:20:22.56 Hand.close @16:20:23.45 Forearm.seek :retract @16:20:24.51 Elbow.reconfigure :tight @16:20:24.62 Elbow.seek PC @16:20:26.21 Elbow.reconfigure :loose @16:20:26.48 Forearm.reconfigure :tight @16:20:26.58 Forearm.seek PC @16:20:28.31 PC.seek :closed @16:20:31.62 upsync @16:20:33.07 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.61 @16:20:33.56 Hand.open @16:20:34.36 Forearm.reconfigure :loose @16:20:34.41 Forearm.seek :retract @16:20:35.36 PSR.select :air @16:20:35.38 PSV.dial PSR @16:20:35.84 Pulling 0.5ml of PSR.flush @16:20:35.85 PSR.select :flush @16:20:35.98 PS.seek 0.5ml @16:20:41.89 delay 5 seconds @16:20:46.92 PSR.select :air @16:20:47.04 PS.seek 2.5ml @16:21:08.29 delay 1 second @16:21:09.31 PRV.dial :waste2,avoiding: :ab2 @16:21:11.17 PTV.dial :PRVmixing @16:21:12.48 PSV.dial :mixloop @16:21:13.53 PS.seek :empty @16:21:39.95 PSR.select :air @16:21:39.97 PSV.dial PSR @16:21:41.14 Pulling 0.5ml of PSR.flush @16:21:41.15 PSR.select :flush @16:21:41.27 PS.seek 0.5ml @16:21:47.26 delay 5 seconds @16:21:52.28 PSR.select :air @16:21:52.40 PS.seek 2.5ml @16:22:13.86 delay 1 second @16:22:14.88 PBV.dial :waste1 @16:22:15.25 PTV.dial :mixing @16:22:16.00 PSV.dial :mixloop @16:22:17.03 PS.seek :empty @16:22:43.45 PSV.dial PSR @16:22:44.50 PSR.select :flush @16:22:44.62 PS.seek 1ml @16:22:55.70 PSR.select :air @16:22:55.82 PS.seek 2.5ml @16:23:11.98 PSV.dial PTV @16:23:12.73 PTV.dial :puck @16:23:13.46 PBV.dial :waste1 @16:23:13.64 PS.seek 0ml @16:23:39.95 PSV.dial PSR @16:23:40.70 PSR.select :flush @16:23:40.82 PS.seek 0.5ml @16:23:46.70 PSR.select :air @16:23:46.82 PS.seek 2.5ml @16:24:08.04 PSV.dial PTV @16:24:08.79 PBV.dial PSV @16:24:09.34 PS.seek 0ml @16:24:35.88 PSV.dial PBV @16:24:36.36 PS.seek 2.5ml @16:25:02.76 PSV.dial :waste1 @16:25:03.94 PS.seek 0ml @16:25:30.35 PSV.dial PSR @16:25:30.82 PSR.select :air @16:25:30.85 PS.seek 2.5ml @16:25:57.38 PSV.dial PTV @16:25:58.12 PBV.dial PSV @16:25:58.31 PS.seek 0ml @16:26:24.66 PSV.dial PBV @16:26:25.14 PS.seek 2.5ml @16:26:51.44 PSV.dial :waste1 @16:26:52.18 PS.seek 0ml @16:27:18.57 PSV.dial PSR @16:27:19.15 PSR.select :flush @16:27:19.27 PS.seek 1ml @16:27:30.38 PSR.select :air @16:27:30.50 PS.seek 2.5ml @16:27:46.63 PSV.dial PTV @16:27:47.51 PTV.dial :puck @16:27:47.69 PBV.dial :waste1 @16:27:48.23 PS.seek 0ml @16:28:14.53 PSV.dial PSR @16:28:15.27 PSR.select :flush @16:28:15.39 PS.seek 0.5ml @16:28:21.31 PSR.select :air @16:28:21.44 PS.seek 2.5ml @16:28:42.88 PSV.dial PTV @16:28:43.75 PBV.dial PSV @16:28:44.31 PS.seek 0ml @16:29:10.66 PSV.dial PBV @16:29:11.12 PS.seek 2.5ml @16:29:37.56 PSV.dial :waste1 @16:29:38.31 PS.seek 0ml @16:30:04.59 PSV.dial PSR @16:30:05.17 PSR.select :air @16:30:05.20 PS.seek 2.5ml @16:30:31.66 PSV.dial PTV @16:30:32.53 PBV.dial PSV @16:30:32.62 PS.seek 0ml @16:30:59.00 PSV.dial PBV @16:30:59.46 PS.seek 2.5ml @16:31:25.91 PSV.dial :waste1 @16:31:26.65 PS.seek 0ml @16:31:53.03 Garaging FlushPuck in Processing Clamp @16:31:53.49 Elbow.reconfigure :tight @16:31:53.60 Elbow.seek PC @16:31:53.72 Elbow.reconfigure :loose @16:31:53.83 Forearm.seek PC @16:31:54.80 Hand.close @16:31:55.42 PC.seek :open @16:32:00.15 Forearm.seek :retract @16:32:01.10 Elbow.reconfigure :tight @16:32:01.15 Elbow.seek :garage @16:32:02.44 Elbow.reconfigure :loose @16:32:02.54 Forearm.reconfigure :tight @16:32:02.59 Forearm.seek :garage @16:32:03.63 Hand.open @16:32:04.35 Forearm.reconfigure :loose @16:32:04.40 Forearm.seek :retract @16:43:31.48 Session Begun @16:43:47.23 -> MainThread.reume @16:43:47.25 NoMethodError in dave -- undefined method `reume' for # @16:43:51.36 -> MainThread.resume @16:43:51.37
Resuming Thread (suspended since 15:41:44) @16:43:51.40 Pumping wcr puck from tube 2 up to 35psi... # @16:43:51.45 CBV.dial SSV @16:43:52.90 CBV.dial between CSV and waste2b @16:43:53.86 CSV.dial between air and CTV @16:43:54.20 CS.reconfigure :fast @16:43:54.27 CS.seek 5.552ml @16:44:08.90 CTV.dial CSV,avoiding: :air @16:44:08.93
CSV.dial CTV @16:44:09.28 CS.reconfigure :pressurize @16:44:09.35 CS.seek 0ml @16:44:24.18 CSV.dial :air @16:44:24.65 CS.reconfigure :fast @16:44:24.73 CS.seek 10ml @16:44:49.39 CSV.dial between air and CTV @16:44:49.73 CS.seek 5.622ml @16:45:00.46 CTV.dial CSV,avoiding: :air @16:45:00.49
CSV.dial CTV @16:45:01.20 CS.reconfigure :pressurize @16:45:01.34 CS.seek 0ml @16:45:16.24 CSV.dial :air @16:45:16.72 CS.reconfigure :fast @16:45:16.80 CS.seek 10ml @16:45:41.42 CSV.dial between air and CTV @16:45:41.76 CS.seek 4.974ml @16:45:54.03 CTV.dial CSV,avoiding: :air @16:45:54.06
CSV.dial CTV @16:45:54.40 CS.reconfigure :pressurize @16:45:54.48 CS.seek 0ml @16:46:07.80 CSV.dial :air @16:46:08.28 CS.reconfigure :fast @16:46:08.35 CS.seek 10ml @16:46:32.76 CSV.dial between air and CTV @16:46:33.11 CS.seek 5.071ml @16:46:45.15 CTV.dial CSV,avoiding: :air @16:46:45.18
CSV.dial CTV @16:46:45.53 CS.reconfigure :pressurize @16:46:45.60 CS.seek 0ml @16:46:59.08 CSV.dial :air @16:46:59.55 CS.reconfigure :default @16:46:59.62 CS.hold @16:46:59.77 ShallowSampler::Error in MAIN -- Puck.from 2, :wcr reached only 12.5 of 35psi after pumping in 40ml of gas @16:46:59.79 Waiting to Resume from Checkpoint "Pumping wcr puck from tube 2 up to 35psi..." @16:46:59.82 BadNews.email "Puck.from 2, :wcr reached only 12.5 of 35psi after pumping in 40ml of gas",Subject: "ShallowSampler::Error in MAIN" @16:47:04.03 upsync @16:49:44.99 -> CC.unloadPuck 1, :WCR @16:49:45.08 Puck::Warning in dave -- Unloading Puck.from 2, :wcr to Storage Carousel @16:49:45.11 Storing WCR puck in Collection Clamp to tube 1 @16:49:45.56 Elbow.reconfigure :tight @16:49:45.64 Elbow.seek 1 @16:49:47.98 Forearm.reconfigure :tight @16:49:48.06 Forearm.seek 1 + 450 counts @16:49:49.11 SE.reconfigure :pick @16:49:49.19 SE.seek :beamBreak @16:49:52.13 SE.configure :default @16:49:52.31 Elbow.reconfigure :limp @16:49:52.38 Forearm.reconfigure :loose @16:49:52.44 Forearm.seek 1 @16:49:52.73 Elbow.hold @16:49:52.81 Elbow.reconfigure :tight @16:49:52.94 SE.seek 2.15 Pucks below Top Plate @16:49:53.44 Forearm.force -20 @16:49:53.50 Forearm.seek 1 + 294 counts @16:49:53.80 Forearm.reconfigure :tight @16:49:53.87 Forearm.seek 1 + 450 counts @16:49:54.11 SE.seek 3.15 Pucks below Top Plate @16:49:54.70 Forearm.reconfigure :loose @16:49:54.77 Forearm.seek :retract @16:49:55.72 Elbow.seek CC @16:49:57.42 Elbow.reconfigure :loose @16:49:57.54 Forearm.seek CC @16:49:58.49 Hand.close @16:49:59.15 CC.seek :open @16:50:03.93 Forearm.seek :retract @16:50:04.93 Elbow.reconfigure :tight @16:50:05.00 Elbow.seek 1 @16:50:06.92 Elbow.reconfigure :limp @16:50:06.99 Forearm.seek 1 @16:50:07.99 Elbow.hold @16:50:08.08 Elbow.reconfigure :tight @16:50:08.15 Hand.open @16:50:08.90 Forearm.force -20 @16:50:08.96 Forearm.seek 1 + 311 counts @16:50:09.26 Forearm.reconfigure :tight @16:50:09.34 Forearm.seek 1 + 450 counts @16:50:10.06 SE.seek :down @16:50:11.19 Forearm.reconfigure :loose @16:50:11.26 Forearm.seek :retract @16:50:12.58 SE.stop @16:50:17.86 -> CC Collection Clamp open @16:50:24.61 -> exit @16:50:24.63 Session Ended + ESPwhich DA_WCR_011024.rb + fn=DA_WCR_011024.rb + IFS=: + set /home/esp/esp2/mission . + fullPath=/home/esp/esp2/mission/DA_WCR_011024.rb + [ -f /home/esp/esp2/mission/DA_WCR_011024.rb ] + realpath /home/esp/esp2/mission/DA_WCR_011024.rb + return 0 + scriptPath=/home/esp/esp2local/mission/DA_WCR_011024.rb + exec ruby /home/esp/esp2/lib/esp.rb /home/esp/esp2local/mission/DA_WCR_011024.rb @18:16:10.38UTC17-Jan-24
ESP2 v3.42 I2C Gateway v3.20 for core bus @18:16:13.01 Configuration for short puck jake 1/11/21 by brent@mbari.org /home/esp/esp2/type/shallow/jake/configure.rb @18:16:16.65 Polling Can every 10:00 @18:16:16.71 Loading /home/esp/esp2local/mission/DA_WCR_011024.rb mission @18:16:16.76 GoodNews.email "Starting DA_WCR_011024 w/pucks in tube 2\n{}\nCTD data unavailable\nCan@18:16:16, 25% flow, 21.6C, 12% humidity, 14.8psia, 13.793V, 0.296A, 50.371Ah, 4.09W",Subject: "DA_WCR_011024 begun" @18:16:16.81
Preparing to sleep until + 00:00:05 @18:16:16.84 Asleep til + 00:00:05 @18:16:16.85 Continuing at 18:16 @18:16:17.14 Gate.power :core,:OFF @18:16:17.16 Gate.power :raw,:OFF @18:16:17.17 Shutdown @18:16:19.71 GoodNews.email "Then, in tube 2: initialPurge; da [50, 50] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_011024.rb:14\n{1 => 0,\n 2 => 4,\n 3 => 0,\n 4 => 0,\n 5 => 0,\n 6 => 0,\n 7 => 0}\nCTD data unavailable\nCan@18:16:16, 25% flow, 21.6C, 12% humidity, 14.8psia, 13.793V, 0.296A, 50.371Ah, 4.09W",Subject: "Asleep until 18:16" @18:16:21.40 upsync @18:16:21.76
Powering core bus @18:16:24.53 upsync @18:16:27.19
Gate.power :raw,:ON @18:16:28.63 CS.configure :default @18:16:28.68 PS.configure :default @18:16:28.72 SS.configure :default @18:16:28.77 CC.configure :default @18:16:28.81 PC.configure :default @18:16:28.88 Forearm.configure :loose @18:16:28.93 Elbow.configure :loose @18:16:29.00 SC.configure :loose @18:16:29.04 SE.configure :default @18:16:29.09 CH.configure :default @18:16:29.12 PH.configure :default @18:16:29.16 All dwarf controllers running firmware version 3.85 @18:16:29.28 Valve.reset @18:16:29.53 Intake.select :close @18:16:29.59 Exhaust.select :close @18:16:29.61 CTV.dial :air @18:16:29.69 PSV.dial :air @18:16:29.82 CSV.dial :waste1 @18:16:29.85
Forearm.home.seek :retract @18:16:30.50 PS.home.seek 0ml @18:16:30.58 SSV.dial :spit,avoiding: :exhaust @18:16:30.65 Hand.close @18:16:30.68
Elbow.reconfigure :loose @18:16:30.77 Elbow.jog 1500 @18:16:31.28 Elbow.reconfigure :loose @18:16:31.34 Elbow.home.seek :clear @18:16:31.63 CS.reconfigure :fast @18:16:31.67 SS.home.seek 0ml @18:16:31.74 CS.home.seek 0ml @18:16:34.57
SE.home.seek :down @18:16:35.22 SC.reconfigure :tight @18:16:35.26 SC.home.seek :initial @18:16:38.76 PS.stop @18:17:00.47
SC.configure :limp @18:17:00.53 Forearm.seek :retract @18:17:00.59 Elbow.seek :hammer @18:17:01.70 Forearm.reconfigure :tight @18:17:01.75 Forearm.seek retracted @18:17:02.00 PC.home.seek :open @18:17:02.04
CC.home.seek :open @18:17:05.47 Phase [initialPurge; da [50, 50] #da and wsr are run simultanelously] @18:17:05.50 Notice.email "At 18:17:05 began in tube 2: initialPurge; da [50, 50] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_011024.rb:14\n{1 => 0,\n 2 => 4,\n 3 => 0,\n 4 => 0,\n 5 => 0,\n 6 => 0,\n 7 => 0}\nCTD data unavailable\nCan@18:17:05, 25% flow, 21.6C, 11% humidity, 14.8psia, 13.671V, 0.302A, 50.376Ah, 4.14W",Subject: "Started DA_WCR_011024 phase initialPurge; da [50, 50] #da and wsr are run simultanelously" @18:17:05.52
Starting initialPurge @18:17:05.55 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @18:17:05.61 Hand.open @18:17:06.33 Elbow.reconfigure :tight @18:17:06.40 Elbow.seek FlushPuck::Garage @18:17:08.34 Elbow.reconfigure :loose @18:17:08.48 Forearm.reconfigure :loose @18:17:08.52 Forearm.seek :garage @18:17:09.42 upsync @18:17:09.48
Hand.close @18:17:10.20 Forearm.seek :retract @18:17:11.20 Elbow.reconfigure :tight @18:17:11.24 Elbow.seek CC @18:17:14.18 Elbow.reconfigure :loose @18:17:14.29 Forearm.reconfigure :tight @18:17:14.34 Forearm.seek CC @18:17:15.57 CC.seek :closed @18:17:20.13 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.37 @18:17:20.21 Hand.open @18:17:20.89 Forearm.reconfigure :loose @18:17:20.93 Forearm.seek :retract @18:17:21.94 Flushing the Collection Valve::Manifold @18:17:22.06 CSV.dial CSR @18:17:23.34 Pulling 5ml of CSR.flush @18:17:23.35 CSR.select :flush @18:17:23.47 CS.reconfigure :default @18:17:23.51 CS.seek 5ml @18:17:37.34 delay 5 seconds @18:17:42.71 CSR.select :air @18:17:42.93 CS.reconfigure :fast @18:17:42.98 CS.seek 7.5ml @18:17:49.87 CSV.dial :waste @18:17:51.15 CS.reconfigure :default @18:17:51.23 CS.seek 0ml @18:18:11.21 Purging external Intake @18:18:11.41 CSV.dial :flush @18:18:12.09 CS.reconfigure :fast @18:18:12.14 CS.seek 10ml @18:18:36.73 delay 5 seconds @18:18:41.75 CSV.dial CBV @18:18:41.78
CBV.dial CSV @18:18:42.59 CTV.dial :intake @18:18:43.35 Intake.select :open @18:18:43.46 CS.seek 0ml @18:19:07.15 Intake.select :close @18:19:07.25 CTV.dial :air @18:19:08.03 Flushing upper manifold @18:19:08.15 CSV.dial :flush @18:19:08.89 CS.seek 5ml @18:19:21.65 delay 5 seconds @18:19:26.67 CSV.dial :air @18:19:27.18 CS.seek 7ml @18:19:32.78 CSV.dial CBV @18:19:32.80
CBV.dial CSV @18:19:33.85 CTV.dial :air @18:19:33.93 CS.seek 0ml @18:19:50.65 delay 7 seconds @18:19:57.68 CS.seek 3ml @18:20:05.86 CSV.dial :waste @18:20:06.33 CS.seek 0ml @18:20:14.16 CSV.dial :flush @18:20:14.61 CS.seek 3ml @18:20:22.83 delay 5 seconds @18:20:27.85 CSV.dial :air @18:20:28.35 CS.seek 5ml @18:20:34.11 CSV.dial :air @18:20:34.29 CS.seek 0ml @18:20:46.65 delay 7 seconds @18:20:53.67 CS.seek 3ml @18:21:01.90 CSV.dial :waste @18:21:02.62 CS.seek 0ml @18:21:10.40 Garaging FlushPuck in Collection Clamp @18:21:10.47 Elbow.reconfigure :tight @18:21:10.52 Elbow.seek CC @18:21:10.77 Elbow.reconfigure :loose @18:21:10.86 Forearm.seek CC @18:21:11.91 Hand.close @18:21:12.58 CC.seek :open @18:21:17.60 Forearm.seek :retract @18:21:18.57 Elbow.reconfigure :tight @18:21:18.62 Elbow.seek :garage @18:21:21.55 Elbow.reconfigure :loose @18:21:21.65 Forearm.reconfigure :tight @18:21:21.70 Forearm.seek :garage @18:21:22.74 Hand.open @18:21:23.38 Forearm.reconfigure :loose @18:21:23.43 Forearm.seek :retract @18:21:24.43 da sampling at most 50ml, wcr at most 50ml @18:21:24.47 DAfans.email "Started da in tube 2 at 18:21:24UTC17-Jan-24\n{1 => 0,\n 2 => 4,\n 3 => 0,\n 4 => 0,\n 5 => 0,\n 6 => 0,\n 7 => 0}\nCTD data unavailable\nCan@18:17:05, 25% flow, 21.6C, 11% humidity, 14.8psia, 13.671V, 0.302A, 50.376Ah, 4.14W",Subject: "da sampling at most 50ml, wcr at most 50ml" @18:21:24.51
Loading sh1 puck from tube 2 into Collection Clamp @18:21:24.77 SC.reconfigure :tight @18:21:24.82 SC.seek 2 @18:21:28.27 upsync @18:21:31.55
SC.configure :limp @18:21:31.78 Elbow.reconfigure :tight @18:21:31.83 Elbow.seek 2 @18:21:33.74 Forearm.reconfigure :tight @18:21:33.79 Forearm.seek 2 + 450 counts @18:21:34.79 SE.reconfigure :pick @18:21:34.84 SE.seek :beamBreak @18:21:37.76 SE.configure :default @18:21:37.92 Elbow.seek 2 @18:21:38.11 Forearm.seek 2 + 300 counts @18:21:38.29 Forearm.reconfigure :loose @18:21:38.34 Hand.close @18:21:38.97 SE.seek :down @18:21:39.18
Forearm.seek :retract @18:21:40.12 Elbow.seek CC @18:21:41.59 SE.stop @18:21:41.77
Elbow.reconfigure :loose @18:21:41.87 Forearm.reconfigure :tight @18:21:41.92 Forearm.seek CC @18:21:43.22 CC.seek :closed @18:21:47.75 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.27 @18:21:47.76 Hand.open @18:21:48.38 Forearm.reconfigure :loose @18:21:48.42 Forearm.seek :retract @18:21:49.41 collectSample 50ml w/bubble point of 24psi @18:21:49.42 Priming 7ml @18:21:49.43 Intake.select :close @18:21:49.45 Exhaust.select :close @18:21:49.46 SS.hold @18:21:49.67 Calibrating pressure and temperature sensors @18:21:49.69 CTV.dial :air @18:21:49.71 SSV.dial :spit,avoiding: :exhaust @18:21:49.79 SS.reconfigure :fast @18:21:49.84 SS.seek 5ml @18:21:52.72 SSV.dial CBV @18:21:52.75
CBV.dial SSV @18:21:54.10 delay 3.8 seconds @18:21:58.02 Sensor limits: Intake max 115.1psia; Outlet max 107.6psia @18:21:58.03 SSV.dial :spit @18:21:59.35 SS.seek 0ml @18:22:02.14 SSV.dial CBV @18:22:03.46 CTV.dial :intake @18:22:04.24 SSV.dial CBV @18:22:04.27
CBV.dial SSV @18:22:04.35 Allowing 40s for 7.0ml of remaining 7.0ml @18:22:04.36 Intake.select :open @18:22:06.52 Intake @15.2psia, vacuum from 9.7 to 12.7psi @18:22:06.54 SS.reconfigure :inhaling 2.20ml/s @18:22:06.59 SS.seek 7ml @18:22:13.65 Stroke completed -- waiting for pressure to equalize ... @18:22:13.66 SS.reconfigure :exhale @18:22:13.71 SS.hold @18:22:17.56 Intake.select :close @18:22:17.72 SSV.dial :exhaust @18:22:18.47 Exhaust.select :open @18:22:18.58 SS.seek 0ml @18:22:22.86 Exhaust.select :close @18:22:22.96 Sampling 50.0ml @18:22:22.97 Intake.select :close @18:22:22.99 Exhaust.select :close @18:22:23.00 SS.reconfigure :default @18:22:23.04 SS.hold @18:22:23.24 CTV.dial :intake @18:22:23.41 SSV.dial CBV @18:22:23.44
CBV.dial SSV @18:22:24.20 Allowing 130s for 25.0ml of remaining 50.0ml @18:22:24.22 Intake.select :open @18:22:26.38 Intake @15.4psia, vacuum from 9.9 to 12.9psi @18:22:26.40 SS.reconfigure :inhaling 2.20ml/s @18:22:26.44 SS.seek 25ml @18:22:38.15 Vacuum too high -- waiting up to 40 seconds @18:22:38.16 SS.reconfigure :exhale @18:22:38.22 SS.hold @18:22:39.50 Intake @12.6psia, vacuum from 7.1 to 10.1psi @18:22:39.51 SS.reconfigure :inhaling 1.76ml/s @18:22:39.56 SS.seek 25ml @18:22:43.59 Stroke completed -- waiting for pressure to equalize ... @18:22:43.60 SS.reconfigure :exhale @18:22:43.65 SS.hold @18:22:49.27 Intake.select :close @18:22:49.42 SSV.dial :exhaust @18:22:50.58 Exhaust.select :open @18:22:50.69 SS.seek 0ml @18:22:59.50 Exhaust.select :close @18:22:59.61 SSV.dial CBV @18:22:59.63
CBV.dial SSV @18:23:00.39 Allowing 130s for 25.0ml of remaining 25.0ml @18:23:00.40 Intake.select :open @18:23:02.56 Intake @15.5psia, vacuum from 10.0 to 13.0psi @18:23:02.58 SS.reconfigure :inhaling 1.76ml/s @18:23:02.63 SS.seek 25ml @18:23:16.62 Vacuum too high -- waiting up to 40 seconds @18:23:16.63 SS.reconfigure :exhale @18:23:16.68 SS.hold @18:23:19.40 Intake @13.3psia, vacuum from 7.8 to 10.8psi @18:23:19.42 SS.reconfigure :inhaling 1.41ml/s @18:23:19.47 SS.seek 25ml @18:23:24.25 Stroke completed -- waiting for pressure to equalize ... @18:23:24.26 SS.reconfigure :exhale @18:23:24.30 SS.hold @18:23:29.92 Intake.select :close @18:23:30.07 SSV.dial :exhaust @18:23:30.83 Exhaust.select :open @18:23:30.94 SS.seek 0ml @18:23:39.79 Exhaust.select :close @18:23:39.91 Sampled 50.0ml @18:23:39.92 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @18:23:39.93 Intake.select :close @18:23:40.00 CSV.dial :waste @18:23:40.59 CS.seek 0ml @18:23:40.61 SSV.dial CBV,avoiding: :exhaust @18:23:40.67
CBV.dial SSV @18:23:40.81 CSV.dial :air @18:23:41.56 CS.seek 10ml @18:23:42.60
CTV.dial :air @18:23:43.37 delay 15 seconds @18:23:58.39 SSV.dial :spit,avoiding: :exhaust @18:23:59.74 CTV.dial CSV,avoiding: :intake @18:23:59.78
SS.seek 0ml @18:23:59.89 SSV.dial CBV,avoiding: :exhaust @18:24:06.49 SSV.dial between CBV and plug @18:24:06.96 CBV.dial between SSV and waste2 @18:24:07.35 CTV.dial CSV,avoiding: :air @18:24:07.37
CSV.dial CTV @18:24:07.87 CS.reconfigure :pressurize @18:24:07.91 CS.seek 0ml @18:24:26.87 BubblePoint: Intake @24.2psia, Outlet @0.5psia @18:24:26.88 CBV.dial SSV @18:24:27.21 SS.reconfigure :default @18:24:27.26 SS.seek 10ml @18:24:32.92 delay 15 seconds @18:24:48.05 Evacuated: Intake @23.9psia, Outlet @24.1psia @18:24:48.06 SSV.dial :spit,avoiding: :exhaust @18:24:49.11 SS.reconfigure :exhale @18:24:49.16 SS.seek 0ml @18:24:54.18 SS.reconfigure :fast @18:24:54.23 SS.seek 5ml @18:24:57.02 SSV.dial CBV,avoiding: :exhaust @18:24:57.05
CBV.dial SSV @18:24:58.39 CTV.dial :air,avoiding: :intake @18:24:59.72 CSV.dial CTV @18:24:59.76
SS.seek 10ml @18:25:00.87 CSV.dial :air @18:25:01.32 CS.reconfigure :fast @18:25:01.37 CS.seek 0ml @18:25:02.20
SSV.dial :spit,avoiding: :exhaust @18:25:03.52 SS.reconfigure :exhale @18:25:03.57 SS.seek 0ml @18:25:08.61 CS.stop @18:25:08.87
SS.reconfigure :fast @18:25:08.91 SS.seek 5ml @18:25:11.92 SSV.dial CBV,avoiding: :exhaust @18:25:11.95
CBV.dial SSV @18:25:14.27 SSV.dial :spit,avoiding: :exhaust @18:25:15.58 SS.reconfigure :default @18:25:15.63 SS.seek 0ml @18:25:18.73 SS.stop @18:25:18.77 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @18:25:18.78 Intake.select :close @18:25:18.85 CSV.dial :waste @18:25:18.98 SSV.dial CBV,avoiding: :exhaust @18:25:19.02
CBV.dial SSV @18:25:19.67 CS.seek 0ml @18:25:19.74 CSV.dial :air @18:25:20.33
CTV.dial :air @18:25:20.52 delay 15 seconds @18:25:20.59 CS.seek 10ml @18:25:35.54
SSV.dial :spit,avoiding: :exhaust @18:25:36.85 CTV.dial CSV,avoiding: :intake @18:25:36.87
SS.reconfigure :exhale @18:25:36.98 SS.seek 0ml @18:25:37.39 SSV.dial CBV,avoiding: :exhaust @18:25:45.02 SSV.dial between CBV and plug @18:25:45.49 CBV.dial between SSV and waste2 @18:25:45.87 CSV.dial between air and CTV @18:25:46.32 CS.seek 8.287ml @18:25:51.33 CTV.dial CSV,avoiding: :air @18:25:51.36
CSV.dial CTV @18:25:51.69 CS.reconfigure :pressurize @18:25:51.74 CS.seek 0ml @18:26:10.08 BubblePoint: Intake @24.2psia, Outlet @2.0psia @18:26:10.09 CBV.dial SSV @18:26:10.43 SS.reconfigure :default @18:26:10.47 SS.seek 10ml @18:26:16.14 delay 15 seconds @18:26:31.27 Evacuated: Intake @23.8psia, Outlet @24.0psia @18:26:31.28 SSV.dial :spit,avoiding: :exhaust @18:26:32.31 SS.reconfigure :exhale @18:26:32.36 SS.seek 0ml @18:26:37.35 SS.reconfigure :fast @18:26:37.40 SS.seek 5ml @18:26:40.25 SSV.dial CBV,avoiding: :exhaust @18:26:40.29
CBV.dial SSV @18:26:41.61 CTV.dial :air,avoiding: :intake @18:26:42.94 CSV.dial CTV @18:26:42.99
SS.seek 10ml @18:26:44.16 CSV.dial :air @18:26:44.60 CS.reconfigure :fast @18:26:44.65 CS.seek 0ml @18:26:45.40
SSV.dial :spit,avoiding: :exhaust @18:26:46.73 SS.reconfigure :exhale @18:26:46.78 SS.seek 0ml @18:26:48.49 CS.stop @18:26:52.07
SS.reconfigure :fast @18:26:52.11 SS.seek 5ml @18:26:54.98 SSV.dial CBV,avoiding: :exhaust @18:26:55.01
CBV.dial SSV @18:26:57.33 SSV.dial :spit,avoiding: :exhaust @18:26:58.66 SS.reconfigure :default @18:26:58.70 SS.seek 0ml @18:27:02.02 SS.stop @18:27:02.06 Lysing: Adding 2.0ml AqMeOH @18:27:02.07 CBV.dial between CSV and waste1,avoiding: :DBV @18:27:03.55 CTV.dial CSV @18:27:05.28 CSV.dial CSR @18:27:06.07 Pulling 1ml of CSR.AqMeOH @18:27:06.08 CSR.select :AqMeOH @18:27:06.20 CS.reconfigure :default @18:27:06.24 CS.seek 1ml @18:27:09.67 delay 5 seconds @18:27:14.69 CSR.select :air @18:27:14.86 CS.seek 6ml @18:27:28.58 delay 2 seconds @18:27:30.59 CSV.dial :waste2 @18:27:31.03 CS.reconfigure :fast @18:27:31.08 CS.seek 0ml @18:27:45.51 CSV.dial CSR @18:27:45.97 Pulling 2ml of CSR.AqMeOH @18:27:45.98 CSR.select :AqMeOH @18:27:46.09 CS.reconfigure :default @18:27:46.13 CS.seek 2ml @18:27:52.45 delay 5 seconds @18:27:57.47 CSR.select :air @18:27:57.64 CS.seek 5ml @18:28:06.11 delay 2 seconds @18:28:08.12 CSV.dial CTV @18:28:08.57 CS.seek 1ml @18:28:19.93 Before lysing Collection Puck Heater at 21.6C/25.4psia @18:28:19.95 Heating Puck.from 2, :sh1 in Collection Clamp to 70C for 10:00 @18:28:19.97 CH.seek 70C,8:00 @18:30:11.55 CH.hold 10:00,70C @18:30:11.75 Begun lysing Collection Puck Heater at 68.1C/30.7psia @18:40:11.75 Ended lysing Collection Puck Heater at 70C/29.4psia @18:40:11.77 CS.seek 0ml @18:40:15.38 CTV.dial between spare and air,avoiding: :intake @18:40:16.39 CSV.dial :air @18:40:16.84 CS.seek 5ml @18:40:30.53 CSV.dial CTV @18:40:30.99 delay 2 seconds @18:40:33.00 CSV.dial :air @18:40:33.45 delay 2 seconds @18:40:35.47 CSV.dial CTV @18:40:35.93 delay 2 seconds @18:40:37.95 CSV.dial :air @18:40:38.39 delay 2 seconds @18:40:40.41 CS.seek 0ml @18:40:54.15 Puck @58.9psia after final lysis pressurization @18:40:54.16 Recovering Lysate into Collection syringe @18:40:54.17 CSV.dial CBV @18:40:55.17 CBV.dial CSV @18:40:55.54 CS.seek 4.999ml @18:41:09.13 delay 5 seconds @18:41:14.14 CTV.dial :air @18:41:14.66 CS.seek 7ml @18:41:20.62 delay 5 seconds @18:41:25.64 CSV.dial :air2 @18:41:26.08 CS.seek 10ml @18:41:34.53 CSV.dial between air and CTV,avoiding: CBV @18:41:35.68 Lysing Complete @18:41:35.75 Storing sh1 puck in Collection Clamp to tube 1 @18:41:35.92 SC.reconfigure :tight @18:41:35.96 SC.seek 1 @18:41:39.54 SC.configure :limp @18:41:39.70 SE.reconfigure :place @18:41:39.77
Elbow.reconfigure :tight @18:41:39.81 SE.seek :beamBreak @18:41:39.84
Elbow.seek CC @18:41:40.65 Elbow.reconfigure :loose @18:41:40.75 Forearm.seek CC @18:41:41.80 Hand.close @18:41:42.43 CC.seek :open @18:41:42.68 SE.configure :default @18:41:42.73 SE.seek :place @18:41:47.17
Forearm.seek :retract @18:41:48.21 Elbow.reconfigure :tight @18:41:48.26 Elbow.seek 1 @18:41:49.95 Elbow.reconfigure :limp @18:41:50.00 Forearm.seek 1 @18:41:51.04 Elbow.hold @18:41:51.10 Elbow.reconfigure :tight @18:41:51.15 Hand.open @18:41:51.92 Forearm.force -20 @18:41:51.97 Forearm.seek 1 + 316 counts @18:41:52.25 Forearm.reconfigure :tight @18:41:52.30 Forearm.seek 1 + 450 counts @18:41:52.55 SE.seek :down @18:41:53.68
Forearm.reconfigure :loose @18:41:53.73 Forearm.seek :retract @18:41:55.23 SE.stop @18:41:55.27
Tranferring DA @18:41:55.28 Beginning samplePassC @18:41:55.30 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @18:41:55.48 Elbow.seek FlushPuck::Garage @18:41:57.27 Elbow.reconfigure :loose @18:41:57.37 Forearm.seek :garage @18:41:58.32 Hand.close @18:41:59.04 Forearm.seek :retract @18:42:00.46 Elbow.reconfigure :tight @18:42:00.51 Elbow.seek CC @18:42:03.38 Elbow.reconfigure :loose @18:42:03.48 Forearm.reconfigure :tight @18:42:03.53 Forearm.seek CC @18:42:04.77 CC.seek :closed @18:42:09.29 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.33 @18:42:09.36 Hand.open @18:42:09.99 Forearm.reconfigure :loose @18:42:10.04 Forearm.seek :retract @18:42:10.99 PSV.dial PSR @18:42:12.35 Pulling 1.5ml of PSR.flush @18:42:12.36 PSR.select :flush @18:42:12.48 PS.seek 1.5ml @18:42:28.70 delay 5 seconds @18:42:33.72 PSV.dial :waste1 @18:42:34.19 PS.seek 0ml @18:42:50.35 PSV.dial PSR @18:42:50.81 PSR.select :air @18:42:50.93 PS.seek 2.5ml @18:43:17.42 PRV.dial CBV,avoiding: :ab2 @18:43:17.46
CBV.dial PRV @18:43:18.23 PSV.dial :mixloop @18:43:19.26 CTV.dial :air,avoiding: :intake @18:43:19.34 PTV.dial :PRVmixing @18:43:20.09 PS.seek 0ml @18:43:46.41 PSV.dial :air @18:43:47.00 PS.seek 2.5ml @18:44:13.35 PSV.dial :mixloop @18:44:13.81 PS.seek 0ml @18:44:40.13 PSV.dial :air @18:44:40.61 PS.seek 2.5ml @18:45:06.95 PSV.dial :mixloop @18:45:07.42 PS.seek 0ml @18:45:33.73 Garaging FlushPuck in Collection Clamp @18:45:33.80 Elbow.reconfigure :tight @18:45:33.84 Elbow.seek CC @18:45:34.11 Elbow.reconfigure :loose @18:45:34.21 Forearm.seek CC @18:45:35.22 Hand.close @18:45:35.80 CC.seek :open @18:45:40.43 Forearm.seek :retract @18:45:41.46 Elbow.reconfigure :tight @18:45:41.51 Elbow.seek :garage @18:45:44.42 Elbow.reconfigure :loose @18:45:44.52 Forearm.reconfigure :tight @18:45:44.57 Forearm.seek :garage @18:45:45.64 Hand.open @18:45:46.36 Forearm.reconfigure :loose @18:45:46.41 Forearm.seek :retract @18:45:47.38 PSV.dial :mixloop @18:45:47.46 PTV.dial :PRVmixing @18:45:47.54 PRV.dial CBV @18:45:47.57
CBV.dial PRV @18:45:47.76 CTV.dial :air,avoiding: :intake @18:45:47.84 Loading lyfil puck from tube 2 into Collection Clamp @18:45:48.08 SC.reconfigure :tight @18:45:48.12 SC.seek 2 @18:45:51.72 SC.configure :limp @18:45:51.93 Elbow.reconfigure :tight @18:45:51.98 Elbow.seek 2 @18:45:53.90 Forearm.reconfigure :tight @18:45:53.95 Forearm.seek 2 + 450 counts @18:45:55.36 SE.reconfigure :pick @18:45:55.45 SE.seek :beamBreak @18:45:58.41 SE.configure :default @18:45:58.57 Elbow.seek 2 @18:45:58.76 Forearm.seek 2 + 300 counts @18:45:59.00 Forearm.reconfigure :loose @18:45:59.05 Hand.close @18:45:59.69 SE.seek :down @18:45:59.89
Forearm.seek :retract @18:46:00.91 Elbow.seek CC @18:46:02.35 SE.stop @18:46:02.57
Elbow.reconfigure :loose @18:46:02.67 Forearm.reconfigure :tight @18:46:02.72 Forearm.seek CC @18:46:04.00 CC.seek :closed @18:46:08.52 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.11 @18:46:08.53 Hand.open @18:46:09.24 Forearm.reconfigure :loose @18:46:09.29 Forearm.seek :retract @18:46:10.27 CTV.dial CSV,avoiding: :intake @18:46:11.62 CSV.dial CTV @18:46:11.94 PS.seek 2.5ml @18:46:38.37 CS.seek 4.5ml @18:46:53.31 delay 8 seconds @18:47:01.33 PSV.dial :air @18:47:02.24 delay 2 seconds @18:47:04.26 PSV.dial :waste2 @18:47:05.00 PS.seek 0ml @18:47:31.41 CBV.dial between PRV and waste1 @18:47:31.74 CSV.dial :air @18:47:32.20 CS.seek 10ml @18:47:47.05 CSV.dial CTV @18:47:47.51 CS.seek 0ml @18:48:14.15 CBV.dial :waste1 @18:48:14.45 delay 5 seconds @18:48:19.47 CTV.dial :air,avoiding: :intake @18:48:20.85 Storing lyfil puck in Collection Clamp to tube 1 @18:48:21.02 SC.reconfigure :tight @18:48:21.07 SC.seek 1 @18:48:24.63 SC.configure :limp @18:48:24.85 Elbow.reconfigure :tight @18:48:24.89 Elbow.seek 1 @18:48:26.64 Forearm.reconfigure :tight @18:48:26.68 Forearm.seek 1 + 450 counts @18:48:27.75 SE.reconfigure :pick @18:48:27.80 SE.seek :beamBreak @18:48:30.83 SE.configure :default @18:48:30.99 Elbow.reconfigure :limp @18:48:31.04 Forearm.reconfigure :loose @18:48:31.08 Forearm.seek 1 @18:48:31.36 Elbow.hold @18:48:31.42 Elbow.reconfigure :tight @18:48:31.52 SE.seek 0.17 Pucks below Top Plate @18:48:31.94 Forearm.force -20 @18:48:31.99 Forearm.seek 1 + 306 counts @18:48:32.69 Forearm.reconfigure :tight @18:48:32.76 Forearm.seek 1 + 450 counts @18:48:32.94 SE.seek 1.17 Pucks below Top Plate @18:48:33.43 Forearm.reconfigure :loose @18:48:33.48 Forearm.seek :retract @18:48:34.49 Elbow.seek CC @18:48:36.17 Elbow.reconfigure :loose @18:48:36.27 Forearm.seek CC @18:48:37.20 Hand.close @18:48:37.80 CC.seek :open @18:48:42.57 Forearm.seek :retract @18:48:43.57 Elbow.reconfigure :tight @18:48:43.62 Elbow.seek 1 @18:48:45.40 Elbow.reconfigure :limp @18:48:45.45 Forearm.seek 1 @18:48:46.42 Elbow.hold @18:48:46.49 Elbow.reconfigure :tight @18:48:46.54 Hand.open @18:48:47.28 Forearm.force -20 @18:48:47.32 Forearm.seek 1 + 306 counts @18:48:47.63 Forearm.reconfigure :tight @18:48:47.68 Forearm.seek 1 + 450 counts @18:48:47.93 SE.seek :down @18:48:49.07
Forearm.reconfigure :loose @18:48:49.11 Forearm.seek :retract @18:48:50.54 SE.stop @18:48:50.58
PSV.dial :air @18:48:51.87 PS.seek 2.5ml @18:49:18.41 delay 1 second @18:49:19.42 PSV.dial :mixloop @18:49:19.90 delay 1 second @18:49:20.91 PSV.dial :air @18:49:21.38 delay 1 second @18:49:22.39 PSV.dial :mixloop @18:49:22.86 delay 1 second @18:49:23.88 PSV.dial :air @18:49:24.35 delay 1 second @18:49:25.37 PS.seek 0ml @18:49:51.75 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @18:49:51.94 Elbow.seek FlushPuck::Garage @18:49:53.74 Elbow.reconfigure :loose @18:49:53.84 Forearm.seek :garage @18:49:54.87 Hand.close @18:49:55.67 Forearm.seek :retract @18:49:56.69 Elbow.reconfigure :tight @18:49:56.74 Elbow.seek PC @18:49:58.02 Elbow.reconfigure :loose @18:49:58.12 Forearm.reconfigure :tight @18:49:58.17 Forearm.seek PC @18:49:59.49 PC.seek :closed @18:50:04.10 Processing Clamp onSpring/offSpring for 16/26 samples, on/off torque 1.56 @18:50:04.17 Hand.open @18:50:04.86 Forearm.reconfigure :loose @18:50:04.90 Forearm.seek :retract @18:50:05.92 PRV.dial :air @18:50:06.82 PSV.dial :mixloop @18:50:07.35 PS.seek 0.3ml @18:50:11.19 PSV.dial PSR @18:50:12.24 Pulling 0.4ml of PSR.air @18:50:12.25 PSR.select :air @18:50:12.28 PS.seek 0.7ml @18:50:17.16 delay 3 seconds @18:50:20.17 PSR.select :air @18:50:20.20 PS.seek 2.5ml @18:50:39.38 delay 3 seconds @18:50:42.39 PSV.dial :mixloop @18:50:43.44 PTV.dial :mixing @18:50:44.19 PBV.dial :waste2 @18:50:45.13 PS.seek 0ml @18:51:11.47 Garaging FlushPuck in Processing Clamp @18:51:11.54 Elbow.reconfigure :tight @18:51:11.59 Elbow.seek PC @18:51:11.71 Elbow.reconfigure :loose @18:51:11.81 Forearm.seek PC @18:51:12.78 Hand.close @18:51:13.42 PC.seek :open @18:51:18.15 Forearm.seek :retract @18:51:19.17 Elbow.reconfigure :tight @18:51:19.22 Elbow.seek :garage @18:51:20.58 Elbow.reconfigure :loose @18:51:20.69 Forearm.reconfigure :tight @18:51:20.74 Forearm.seek :garage @18:51:21.76 Hand.open @18:51:22.48 Forearm.reconfigure :loose @18:51:22.53 Forearm.seek :retract @18:51:23.56 Loading da puck from tube 2 into Processing Clamp @18:51:23.80 SC.reconfigure :tight @18:51:23.84 SC.seek 2 @18:51:27.38 SC.configure :limp @18:51:27.59 Elbow.reconfigure :tight @18:51:27.65 Elbow.seek 2 @18:51:29.91 Forearm.reconfigure :tight @18:51:29.96 Forearm.seek 2 + 450 counts @18:51:31.03 SE.reconfigure :pick @18:51:31.08 SE.seek :beamBreak @18:51:34.14 SE.configure :default @18:51:34.30 Elbow.seek 2 @18:51:34.49 Forearm.seek 2 + 300 counts @18:51:34.90 Forearm.reconfigure :loose @18:51:34.95 Hand.close @18:51:35.56 SE.seek :down @18:51:35.76
Forearm.seek :retract @18:51:36.69 Elbow.seek PC @18:51:38.26 SE.stop @18:51:39.21
Elbow.reconfigure :loose @18:51:39.31 Forearm.reconfigure :tight @18:51:39.36 Forearm.seek PC @18:51:40.72 PC.seek :closed @18:51:45.35 Processing Clamp onSpring/offSpring for 15/25 samples, on/off torque 1.61 @18:51:45.36 Hand.open @18:51:45.98 Forearm.reconfigure :loose @18:51:46.02 Forearm.seek :retract @18:51:46.99 Heating Puck.from 2, :da in Processing Clamp to 28C for 2:00:00 @18:51:47.01 PH.seek 28C,5:00 @18:52:14.58 PH.hold 2:00:00,28C @18:52:14.62 delay 1:30 @18:53:44.68 PSR.select :air @18:53:44.69 PSV.dial PSR @18:53:45.73 Pulling 0.5ml of PSR.wash @18:53:45.74 PSR.select :wash @18:53:45.86 PS.seek 0.5ml @18:53:51.84 delay 5 seconds @18:53:56.86 PSR.select :air @18:53:56.98 PS.seek 2ml @18:54:13.09 PTV.dial :puck @18:54:13.82 PSV.dial PTV @18:54:14.70 PBV.dial :waste1 @18:54:16.07 PS.seek 1.5ml @18:54:22.06 delay 3 seconds @18:54:25.08 PBV.dial between PSV and waste1 @18:54:25.44 PS.seek 0ml @18:54:41.59 delay 5:00 @18:59:41.61 recoverPSRreagent08 @18:59:41.62 PTV.dial :puck @18:59:41.69 PBV.dial :waste1 @18:59:42.07 delay 30 seconds @19:00:12.09 PBV.dial between PSV and waste1 @19:00:12.47 PSR.select :air @19:00:12.49 PSV.dial PSR @19:00:13.23 PS.reconfigure :fast @19:00:13.28 PS.seek 2.5ml @19:00:37.14 PSV.dial PTV @19:00:38.02 delay 5 seconds @19:00:43.04 PSV.dial PSR @19:00:43.77 delay 5 seconds @19:00:48.79 PSV.dial PTV @19:00:49.54 delay 5 seconds @19:00:54.56 PSV.dial :air @19:00:55.74 PS.seek 0ml @19:01:19.36 PTV.dial :PRVmixing,via: PRV @19:01:20.66 PSV.dial :mixloop @19:01:21.13 PRV.dial :ab1 @19:01:21.88 PS.reconfigure :slow @19:01:21.93 PS.seek 0.15ml @19:01:25.52 delay 2 seconds @19:01:27.54 CBV.dial PRV @19:01:27.58
PRV.dial CBV @19:01:28.13 PS.seek 0.34ml @19:01:32.46 delay 2 seconds @19:01:34.47 CBV.dial between PRV and PSV @19:01:34.79 PRV.dial :air @19:01:35.24 PS.seek 0.9ml @19:01:47.24 PRV.dial :waste2 @19:01:47.98 PS.seek 0.5ml @19:01:56.61 addPMLReagent08 @19:01:56.62 PSV.dial :air @19:01:57.19 PS.reconfigure :default @19:01:57.24 PS.seek 2ml @19:02:13.39 PSV.dial :PML @19:02:13.85 PTV.dial :mixing @19:02:14.60 PBV.dial :waste1 @19:02:15.03 PS.seek 1.85ml @19:02:17.42 PBV.dial between PSV and waste1 @19:02:17.79 PS.seek 0ml @19:02:37.70 delay 2 seconds @19:02:39.72 Beginning samplePassC @19:02:39.74 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @19:02:39.92 Elbow.reconfigure :tight @19:02:39.97 Elbow.seek FlushPuck::Garage @19:02:41.31 Elbow.reconfigure :loose @19:02:41.41 Forearm.seek :garage @19:02:42.44 Hand.close @19:02:43.17 Forearm.seek :retract @19:02:44.19 Elbow.reconfigure :tight @19:02:44.24 Elbow.seek CC @19:02:47.11 Elbow.reconfigure :loose @19:02:47.21 Forearm.reconfigure :tight @19:02:47.69 Forearm.seek CC @19:02:49.61 CC.seek :closed @19:02:54.14 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.35 @19:02:54.21 Hand.open @19:02:54.85 Forearm.reconfigure :loose @19:02:54.90 Forearm.seek :retract @19:02:55.85 PSV.dial PSR @19:02:56.89 Pulling 1.5ml of PSR.flush @19:02:56.90 PSR.select :flush @19:02:57.02 PS.seek 1.5ml @19:03:13.11 delay 5 seconds @19:03:18.13 PSV.dial :waste1 @19:03:18.60 PS.seek 0ml @19:03:34.73 PSV.dial PSR @19:03:35.20 PSR.select :air @19:03:35.32 PS.seek 2.5ml @19:04:01.71 PRV.dial CBV,avoiding: :ab2 @19:04:01.74
CBV.dial PRV @19:04:03.33 PSV.dial :mixloop @19:04:04.36 CTV.dial :air,avoiding: :intake @19:04:04.44 PTV.dial :PRVmixing @19:04:05.19 PS.seek 0ml @19:04:31.70 PSV.dial :air @19:04:32.16 PS.seek 2.5ml @19:04:58.51 PSV.dial :mixloop @19:04:58.99 PS.seek 0ml @19:05:25.29 PSV.dial :air @19:05:25.77 PS.seek 2.5ml @19:05:52.14 PSV.dial :mixloop @19:05:52.61 PS.seek 0ml @19:06:18.95 Garaging FlushPuck in Collection Clamp @19:06:19.49 Elbow.reconfigure :tight @19:06:19.54 Elbow.seek CC @19:06:19.76 Elbow.reconfigure :loose @19:06:19.86 Forearm.seek CC @19:06:20.83 Hand.close @19:06:21.41 CC.seek :open @19:06:26.03 Forearm.seek :retract @19:06:27.02 Elbow.reconfigure :tight @19:06:27.07 Elbow.seek :garage @19:06:30.03 Elbow.reconfigure :loose @19:06:30.13 Forearm.reconfigure :tight @19:06:30.18 Forearm.seek :garage @19:06:31.20 Hand.open @19:06:31.84 Forearm.reconfigure :loose @19:06:31.89 Forearm.seek :retract @19:06:32.91 delayUntil "19:10:39.71" @19:06:32.94 Whole Cell Archive sampling 50ml @19:06:32.95 Loading wcr puck from tube 2 into Collection Clamp @19:06:33.25 SC.reconfigure :tight @19:06:33.31 SC.seek 2 @19:06:33.68 SC.configure :limp @19:06:33.91 Elbow.reconfigure :tight @19:06:33.96 Elbow.seek 2 @19:06:35.87 Forearm.reconfigure :tight @19:06:35.92 Forearm.seek 2 + 450 counts @19:06:36.95 SE.reconfigure :pick @19:06:37.02 SE.seek :beamBreak @19:06:40.08 SE.configure :default @19:06:40.68 Elbow.seek 2 @19:06:40.87 Forearm.seek 2 + 300 counts @19:06:41.06 Forearm.reconfigure :loose @19:06:41.11 Hand.close @19:06:41.79 Puck::Warning in WCR -- Loaded Last remaining Puck (from Tube 2) @19:06:41.82 SE.seek :down @19:06:42.04 Forearm.seek :retract @19:06:43.05 Elbow.seek CC @19:06:44.64 SE.stop @19:06:44.69 Elbow.reconfigure :loose @19:06:44.81 Forearm.reconfigure :tight @19:06:44.86 Forearm.seek CC @19:06:46.07 CC.seek :closed @19:06:50.59 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.23 @19:06:50.61 Hand.open @19:06:51.22 Forearm.reconfigure :loose @19:06:51.27 Forearm.seek :retract @19:06:52.25 collectSample 50ml w/bubble point of 35psi @19:06:52.27 Priming 7ml @19:06:52.28 Intake.select :close @19:06:52.29 Exhaust.select :close @19:06:52.30 SS.hold @19:06:52.51 Calibrating pressure sensors @19:06:52.52 CTV.dial :air @19:06:52.60 SSV.dial :spit,avoiding: :exhaust @19:06:52.69 SS.reconfigure :fast @19:06:52.74 SS.seek 5ml @19:06:55.89 SSV.dial CBV @19:06:55.92 CBV.dial SSV @19:06:57.28 delay 3.8 seconds @19:07:01.22 Sensor limits: Intake max 115.9psia; Outlet max 107.8psia @19:07:01.23 SSV.dial :spit @19:07:02.55 SS.seek 0ml @19:07:05.38 SSV.dial CBV @19:07:06.70 CTV.dial :intake @19:07:07.45 SSV.dial CBV @19:07:07.48 CBV.dial SSV @19:07:07.62 Allowing 40s for 7.0ml of remaining 7.0ml @19:07:07.63 Intake.select :open @19:07:09.80 Intake @15.3psia, vacuum from 9.8 to 12.8psi @19:07:09.82 SS.reconfigure :inhaling 2.20ml/s @19:07:09.87 SS.seek 7ml @19:07:16.40 Stroke completed -- waiting for pressure to equalize ... @19:07:16.41 SS.reconfigure :exhale @19:07:16.46 SS.hold @19:07:22.10 Intake.select :close @19:07:22.26 SSV.dial :exhaust @19:07:23.01 Exhaust.select :open @19:07:23.12 SS.seek 0ml @19:07:27.28 Exhaust.select :close @19:07:27.83 Sampling 50.0ml @19:07:27.84 Intake.select :close @19:07:27.85 Exhaust.select :close @19:07:27.87 SS.reconfigure :default @19:07:27.91 SS.hold @19:07:28.12 CTV.dial :intake @19:07:28.30 SSV.dial CBV @19:07:28.33 CBV.dial SSV @19:07:29.10 Allowing 130s for 25.0ml of remaining 50.0ml @19:07:29.12 Intake.select :open @19:07:31.29 Intake @15.7psia, vacuum from 10.0 to 13.0psi @19:07:31.31 SS.reconfigure :inhaling 2.20ml/s @19:07:31.36 SS.seek 25ml @19:07:45.49 Stroke completed -- waiting for pressure to equalize ... @19:07:45.50 SS.reconfigure :exhale @19:07:45.55 SS.hold @19:07:51.19 Intake.select :close @19:07:51.34 SSV.dial :exhaust @19:07:52.08 Exhaust.select :open @19:07:52.20 SS.seek 0ml @19:08:00.93 Exhaust.select :close @19:08:01.04 SSV.dial CBV @19:08:01.08 CBV.dial SSV @19:08:01.83 Allowing 130s for 25.0ml of remaining 25.0ml @19:08:01.85 Intake.select :open @19:08:04.42 Intake @15.9psia, vacuum from 10.0 to 13.0psi @19:08:04.47 SS.reconfigure :inhaling 2.20ml/s @19:08:04.52 SS.seek 25ml @19:08:17.87 Vacuum too high -- waiting up to 40 seconds @19:08:17.89 SS.reconfigure :exhale @19:08:17.94 SS.hold @19:08:18.62 Intake @12.7psia, vacuum from 7.2 to 10.2psi @19:08:18.64 SS.reconfigure :inhaling 1.76ml/s @19:08:18.69 SS.seek 25ml @19:08:21.43 Stroke completed -- waiting for pressure to equalize ... @19:08:21.44 SS.reconfigure :exhale @19:08:21.49 SS.hold @19:08:26.37 Intake.select :close @19:08:26.52 SSV.dial :exhaust @19:08:27.27 Exhaust.select :open @19:08:27.39 SS.seek 0ml @19:08:36.25 Exhaust.select :close @19:08:36.37 Sampled 50.0ml @19:08:36.38 Drying puck before fixing for 5:00 @19:08:36.39 CBV.dial :waste2b @19:08:37.15 delay 1:00 @19:09:37.17 CSV.dial :air @19:09:37.63 CS.reconfigure :fast @19:09:37.68 CS.seek 10ml @19:10:02.57 Pumping wcr puck from tube 2 up to 35psi... @19:10:02.58 CBV.dial SSV @19:10:03.85 CBV.dial between CSV and waste2b @19:10:05.24 CSV.dial between air and CTV @19:10:05.57 CS.seek 7.319ml @19:10:12.54 CTV.dial CSV,avoiding: :air @19:10:12.57 CSV.dial CTV @19:10:13.38 CS.reconfigure :pressurize @19:10:13.44 CS.seek 0ml @19:10:27.05 CBV.dial :waste2b @19:10:27.38 CS.reconfigure :default @19:10:27.43 CS.seek 0ml @19:10:33.75 delay 1:00 @19:10:39.73 recoverPRVreagent08 @19:10:39.74 PTV.dial :mixing @19:10:40.48 PBV.dial :waste1 @19:10:40.84 delay 30 seconds @19:11:10.86 PBV.dial between PSV and waste1 @19:11:11.23 PSV.dial :air @19:11:11.70 PS.reconfigure :fast @19:11:11.75 PS.seek 2.5ml @19:11:33.78 CTV.dial :air,avoiding: :intake @19:11:35.10 Fix #1 of 2 (with RNAlater) for 2:30 @19:11:35.11 CSV.dial CSR @19:11:35.22 PSV.dial :mixloop @19:11:35.55 Pulling 2ml of CSR.RNAlater @19:11:35.56 CSR.select :RNAlater @19:11:35.70 CS.seek 2ml @19:11:35.72 delay 2 seconds @19:11:37.74 PSV.dial :air @19:11:38.21 delay 2 seconds @19:11:40.23 PSV.dial :mixloop @19:11:40.69 delay 2 seconds @19:11:42.03 delay 5 seconds @19:11:42.71 PSV.dial :air @19:11:43.18 PS.seek 0ml @19:11:47.05 CSR.select :air @19:11:47.16 CS.reconfigure :fast @19:11:47.21 CS.seek 10ml @19:12:06.26 washCycleDA #1 of 2 @19:12:06.27 PSR.select :air @19:12:06.28 PSV.dial PSR @19:12:07.06 Pumping wcr puck from tube 2 up to 35psi... @19:12:07.08 CBV.dial SSV @19:12:07.61 Pulling 0.5ml of PSR.wash @19:12:07.63 PSR.select :wash @19:12:07.74 PS.reconfigure :default @19:12:07.79 PS.seek 0.5ml @19:12:08.35 CBV.dial between CSV and waste2b @19:12:09.31 CSV.dial between air and CTV @19:12:09.91 CS.seek 7.285ml @19:12:13.79 delay 5 seconds @19:12:17.35 CTV.dial CSV,avoiding: :air @19:12:17.38 CSV.dial CTV @19:12:18.72 CS.reconfigure :pressurize @19:12:18.78 CS.seek 0ml @19:12:18.84 PSR.select :air @19:12:18.96 PS.seek 2ml @19:12:32.71 delay 2:30 @19:12:35.16 PTV.dial :puck @19:12:35.90 PSV.dial PTV @19:12:36.63 PBV.dial :waste1 @19:12:37.05 PS.seek 1.5ml @19:12:43.10 delay 3 seconds @19:12:46.12 PBV.dial between PSV and waste1 @19:12:46.48 PS.seek 0ml @19:13:02.82 delay 2:00 @19:15:02.74 CBV.dial :waste2b @19:15:02.84 recoverPSRreagent08 @19:15:02.85 PTV.dial :puck @19:15:02.92 PBV.dial :waste1 @19:15:03.06 CS.reconfigure :default @19:15:03.11 CS.seek 0ml @19:15:03.28 delay 30 seconds @19:15:09.25 delay 1:00 @19:15:33.31 PBV.dial between PSV and waste1 @19:15:33.67 PSR.select :air @19:15:33.69 PSV.dial PSR @19:15:34.44 PS.reconfigure :fast @19:15:34.50 PS.seek 2.5ml @19:15:58.00 PSV.dial PTV @19:15:58.75 delay 5 seconds @19:16:03.77 PSV.dial PSR @19:16:04.52 delay 5 seconds @19:16:09.27 CTV.dial :air,avoiding: :intake @19:16:09.53 PSV.dial PTV @19:16:10.41 delay 5 seconds @19:16:10.61 Fix #2 of 2 (with RNAlater) for 2:30 @19:16:10.63 CSV.dial CSR @19:16:11.06 Pulling 2ml of CSR.RNAlater @19:16:11.07 CSR.select :RNAlater @19:16:11.19 CS.seek 2ml @19:16:15.43 PSV.dial :air @19:16:16.32 PS.seek 0ml @19:16:17.15 delay 5 seconds @19:16:22.17 CSR.select :air @19:16:22.29 CS.reconfigure :fast @19:16:22.34 CS.seek 10ml @19:16:39.41 washCycleDA #2 of 2 @19:16:39.42 PSR.select :air @19:16:39.44 PSV.dial PSR @19:16:40.89 Pulling 0.5ml of PSR.wash @19:16:40.90 PSR.select :wash @19:16:41.02 PS.reconfigure :default @19:16:41.07 PS.seek 0.5ml @19:16:42.43 Pumping wcr puck from tube 2 up to 35psi... @19:16:42.45 CBV.dial SSV @19:16:43.71 CBV.dial between CSV and waste2b @19:16:44.65 CSV.dial between air and CTV @19:16:45.25 CS.seek 7.306ml @19:16:47.41 delay 5 seconds @19:16:52.22 CTV.dial CSV,avoiding: :air @19:16:52.27 CSV.dial CTV @19:16:52.42 PSR.select :air @19:16:52.54 PS.seek 2ml @19:16:53.58 CS.reconfigure :pressurize @19:16:53.63 CS.seek 0ml @19:17:08.72 PTV.dial :puck @19:17:08.80 PSV.dial PTV @19:17:09.66 PBV.dial :waste1 @19:17:10.07 PS.seek 1.5ml @19:17:12.84 CSV.dial :air @19:17:13.42 CS.reconfigure :fast @19:17:13.47 CS.seek 10ml @19:17:16.06 delay 3 seconds @19:17:19.08 PBV.dial between PSV and waste1 @19:17:19.46 PS.seek 0ml @19:17:35.60 delay 2:00 @19:17:38.42 CSV.dial between air and CTV @19:17:38.74 CS.seek 4.218ml @19:17:52.78 CTV.dial CSV,avoiding: :air @19:17:52.81 CSV.dial CTV @19:17:53.17 CS.reconfigure :pressurize @19:17:53.22 CS.seek 0ml @19:18:04.52 CSV.dial :air @19:18:04.98 CS.reconfigure :fast @19:18:05.04 CS.seek 10ml @19:18:29.67 CSV.dial between air and CTV @19:18:30.00 CS.seek 4.324ml @19:18:44.41 CTV.dial CSV,avoiding: :air @19:18:44.43 CSV.dial CTV @19:18:44.73 CS.reconfigure :pressurize @19:18:44.79 CS.seek 0ml @19:18:56.37 CSV.dial :air @19:18:56.83 CS.reconfigure :fast @19:18:56.88 CS.seek 10ml @19:19:21.52 CSV.dial between air and CTV @19:19:21.84 CS.seek 4.467ml @19:19:35.34 CTV.dial CSV,avoiding: :air @19:19:35.37 CSV.dial CTV @19:19:35.61 recoverPSRreagent08 @19:19:35.62 PTV.dial :puck @19:19:35.70 CS.reconfigure :pressurize @19:19:35.78 CS.seek 0ml @19:19:35.80 PBV.dial :waste1 @19:19:36.20 delay 30 seconds @19:19:47.83 CSV.dial :air @19:19:48.29 CS.reconfigure :default @19:19:48.34 CS.hold @19:19:48.47 ShallowSampler::Error in MAIN -- Puck.from 2, :wcr reached only 26.7 of 35psi after pumping in 40ml of gas @19:19:48.48 Waiting to Resume from Checkpoint "Pumping wcr puck from tube 2 up to 35psi..." @19:19:48.50 BadNews.email "Puck.from 2, :wcr reached only 26.7 of 35psi after pumping in 40ml of gas",Subject: "ShallowSampler::Error in MAIN" @19:19:52.92 upsync @19:20:06.22 PBV.dial between PSV and waste1 @19:20:06.61 PSR.select :air @19:20:06.63 PSV.dial PSR @19:20:07.49 PS.reconfigure :fast @19:20:07.54 PS.seek 2.5ml @19:20:31.06 PSV.dial PTV @19:20:31.79 delay 5 seconds @19:20:36.82 PSV.dial PSR @19:20:37.56 delay 5 seconds @19:20:42.58 PSV.dial PTV @19:20:43.32 delay 5 seconds @19:20:48.35 PSV.dial :air @19:20:49.11 PS.seek 0ml @19:21:12.12 getPMLReagent08 @19:21:12.13 PRV.dial :air @19:21:12.59 delay 5 seconds @19:21:17.61 PTV.dial :PRVmixing @19:21:18.98 delay 5 seconds @19:21:24.00 PRV.dial :ab2 @19:21:24.45 PTV.dial :PRVmixing @19:21:24.53 PSV.dial :mixloop @19:21:25.00 PS.reconfigure :default @19:21:25.05 PS.seek 0.3ml @19:21:28.93 delay 2 seconds @19:21:30.95 PRV.dial :air @19:21:31.48 PS.seek 0.55ml @19:21:34.93 addPMLReagent08 @19:21:34.94 PSV.dial :air @19:21:35.42 PS.seek 2ml @19:21:50.99 PSV.dial :PML @19:21:51.46 PTV.dial :mixing @19:21:52.21 PBV.dial :waste1 @19:21:52.65 PS.seek 1.85ml @19:21:54.96 PBV.dial between PSV and waste1 @19:21:55.34 PS.seek 0ml @19:22:15.02 delay 2 seconds @19:22:17.04 delay 8:00 @19:30:17.07 recoverPRVreagent08 @19:30:17.08 PTV.dial :mixing @19:30:17.15 PBV.dial :waste1 @19:30:17.53 delay 30 seconds @19:30:47.56 PBV.dial between PSV and waste1 @19:30:47.92 PSV.dial :air @19:30:48.52 PS.reconfigure :fast @19:30:48.57 PS.seek 2.5ml @19:31:12.04 PSV.dial :mixloop @19:31:12.50 delay 2 seconds @19:31:14.52 PSV.dial :air @19:31:14.98 delay 2 seconds @19:31:17.00 PSV.dial :mixloop @19:31:17.48 delay 2 seconds @19:31:19.49 PSV.dial :air @19:31:19.96 PS.seek 0ml @19:31:43.20 washCycleDA #1 of 3 @19:31:43.21 PSR.select :air @19:31:43.23 PSV.dial PSR @19:31:44.56 Pulling 0.5ml of PSR.wash @19:31:44.57 PSR.select :wash @19:31:44.69 PS.reconfigure :default @19:31:44.73 PS.seek 0.5ml @19:31:50.70 delay 5 seconds @19:31:55.72 PSR.select :air @19:31:55.84 PS.seek 2ml @19:32:12.04 PTV.dial :puck @19:32:12.78 PSV.dial PTV @19:32:13.64 PBV.dial :waste1 @19:32:14.05 PS.seek 1.5ml @19:32:19.92 delay 3 seconds @19:32:22.94 PBV.dial between PSV and waste1 @19:32:23.30 PS.seek 0ml @19:32:39.39 delay 2:00 @19:34:39.42 recoverPSRreagent08 @19:34:39.43 PTV.dial :puck @19:34:39.49 PBV.dial :waste1 @19:34:39.85 delay 30 seconds @19:35:09.88 PBV.dial between PSV and waste1 @19:35:10.70 PSR.select :air @19:35:10.72 PSV.dial PSR @19:35:11.47 PS.reconfigure :fast @19:35:11.52 PS.seek 2.5ml @19:35:35.04 PSV.dial PTV @19:35:35.81 delay 5 seconds @19:35:40.83 PSV.dial PSR @19:35:41.59 delay 5 seconds @19:35:46.61 PSV.dial PTV @19:35:47.37 delay 5 seconds @19:35:52.39 PSV.dial :air @19:35:53.15 PS.seek 0ml @19:36:16.32 washCycleDA #2 of 3 @19:36:16.33 PSR.select :air @19:36:16.35 PSV.dial PSR @19:36:17.66 Pulling 0.5ml of PSR.wash @19:36:17.67 PSR.select :wash @19:36:17.79 PS.reconfigure :default @19:36:17.84 PS.seek 0.5ml @19:36:23.82 delay 5 seconds @19:36:28.84 PSR.select :air @19:36:28.96 PS.seek 2ml @19:36:45.19 PTV.dial :puck @19:36:45.27 PSV.dial PTV @19:36:46.02 PBV.dial :waste1 @19:36:46.44 PS.seek 1.5ml @19:36:52.38 delay 3 seconds @19:36:55.40 PBV.dial between PSV and waste1 @19:36:55.76 PS.seek 0ml @19:37:11.91 delay 2:00 @19:39:11.94 recoverPSRreagent08 @19:39:11.95 PTV.dial :puck @19:39:12.02 PBV.dial :waste1 @19:39:12.38 delay 30 seconds @19:39:42.41 PBV.dial between PSV and waste1 @19:39:42.78 PSR.select :air @19:39:42.80 PSV.dial PSR @19:39:43.68 PS.reconfigure :fast @19:39:43.73 PS.seek 2.5ml @19:40:07.25 PSV.dial PTV @19:40:07.99 delay 5 seconds @19:40:13.01 PSV.dial PSR @19:40:13.76 delay 5 seconds @19:40:18.78 PSV.dial PTV @19:40:19.52 delay 5 seconds @19:40:24.54 PSV.dial :air @19:40:25.30 PS.seek 0ml @19:40:48.47 washCycleDA #3 of 3 @19:40:48.48 PSR.select :air @19:40:48.50 PSV.dial PSR @19:40:49.81 Pulling 0.5ml of PSR.wash @19:40:49.82 PSR.select :wash @19:40:49.94 PS.reconfigure :default @19:40:49.99 PS.seek 0.5ml @19:40:55.97 delay 5 seconds @19:41:00.99 PSR.select :air @19:41:01.11 PS.seek 2ml @19:41:17.31 PTV.dial :puck @19:41:17.39 PSV.dial PTV @19:41:18.13 PBV.dial :waste1 @19:41:18.56 PS.seek 1.5ml @19:41:24.59 delay 3 seconds @19:41:27.61 PBV.dial between PSV and waste1 @19:41:27.99 PS.seek 0ml @19:41:44.10 delay 2:00 @19:43:44.13 recoverPSRreagent08 PTV.dial :puck @19:43:44.20 PBV.dial :waste1 @19:43:44.58 delay 30 seconds @19:44:14.60 PBV.dial between PSV and waste1 @19:44:14.95 PSR.select :air @19:44:14.97 PSV.dial PSR @19:44:15.74 PS.reconfigure :fast @19:44:15.79 PS.seek 2.5ml @19:44:39.25 PSV.dial PTV @19:44:40.01 delay 5 seconds @19:44:45.04 PSV.dial PSR @19:44:45.78 delay 5 seconds @19:44:50.80 PSV.dial PTV @19:44:51.54 delay 5 seconds @19:44:56.57 PSV.dial :air @19:44:57.44 PS.seek 0ml @19:45:20.49 Gate.power :camera,:ON @19:45:20.51 Mixing DA Substrates @19:45:20.52 PSR.select :air @19:45:20.54 PSV.dial PSR @19:45:21.90 Pulling 0.25ml of PSR.sub1 @19:45:21.93 PSR.select :sub1 @19:45:22.15 PS.reconfigure :default @19:45:22.30 PS.seek 0.25ml @19:45:25.72 delay 5 seconds @19:45:30.74 Pulling 0.25ml of PSR.sub2 @19:45:30.76 PSR.select :sub2 @19:45:30.88 PS.seek 0.5ml @19:45:34.43 delay 5 seconds @19:45:39.45 PSR.select :air @19:45:39.57 PS.seek 2ml @19:45:55.65 PTV.dial :puck @19:45:55.73 PSV.dial PTV @19:45:56.50 PBV.dial :waste1 @19:45:56.91 PS.seek 1.5ml @19:46:02.84 delay 3 seconds @19:46:05.86 PBV.dial between PSV and waste1 @19:46:06.22 PS.seek 0ml @19:46:22.52 delay 10 seconds @19:46:32.55 recoverPSRreagent08 @19:46:32.56 PTV.dial :puck @19:46:32.63 PBV.dial :waste1 @19:46:33.00 delay 30 seconds @19:47:03.02 PBV.dial between PSV and waste1 @19:47:03.38 PSR.select :air @19:47:03.40 PSV.dial PSR @19:47:04.17 PS.reconfigure :fast @19:47:04.22 PS.seek 2.5ml @19:47:28.18 PSV.dial PTV @19:47:28.95 delay 5 seconds @19:47:33.97 PSV.dial PSR @19:47:34.73 delay 5 seconds @19:47:39.75 PSV.dial PTV @19:47:40.51 delay 5 seconds @19:47:45.53 PSV.dial :air @19:47:46.41 PS.seek 0ml @19:48:09.96 Additional air clear @19:48:09.97 PSR.select :air @19:48:09.99 PSV.dial PSR @19:48:11.34 Pulling 2ml of PSR.air @19:48:11.35 PSR.select :air @19:48:11.37 PS.reconfigure :default @19:48:11.41 PS.seek 2ml @19:48:33.12 delay 5 seconds @19:48:38.14 PTV.dial :puck @19:48:38.23 PSV.dial PTV @19:48:38.99 PBV.dial :waste1 @19:48:39.43 PS.seek 1.5ml @19:48:45.34 delay 3 seconds @19:48:48.35 PBV.dial between PSV and waste1 @19:48:48.74 delay 1 second @19:48:49.76 PSV.dial :waste1 @19:48:50.83 PS.seek 0ml @19:49:07.07 Processing Puck Heater at 28C before imaging @19:49:07.08 PH.coast @19:49:07.20 Imaging da puck in Processing Clamp while storing it to tube 1 @19:49:07.37 SC.reconfigure :tight @19:49:07.43 SC.seek 1 @19:49:11.00 SC.configure :limp @19:49:11.22 Elbow.reconfigure :tight @19:49:11.27 Elbow.seek 1 @19:49:13.02 Forearm.reconfigure :tight @19:49:13.07 Forearm.seek 1 + 450 counts @19:49:14.14 SE.reconfigure :pick @19:49:14.19 SE.seek :beamBreak @19:49:17.24 SE.configure :default @19:49:17.40 Elbow.reconfigure :limp @19:49:17.45 Forearm.reconfigure :loose @19:49:17.50 Forearm.seek 1 @19:49:17.70 Elbow.hold @19:49:17.76 Elbow.reconfigure :tight @19:49:17.86 SE.seek 1.17 Pucks below Top Plate @19:49:18.30 Forearm.force -20 @19:49:18.34 Forearm.seek 1 + 316 counts @19:49:18.63 Forearm.reconfigure :tight @19:49:18.68 Forearm.seek 1 + 450 counts @19:49:18.88 SE.seek 2.17 Pucks below Top Plate @19:49:19.44 Forearm.reconfigure :loose @19:49:19.91 Forearm.seek :retract @19:49:20.94 Elbow.seek PC @19:49:23.44 Elbow.reconfigure :loose @19:49:23.55 Forearm.seek PC @19:49:24.54 Hand.close @19:49:25.12 PC.seek :open @19:49:29.88 Forearm.seek :retract @19:49:30.84 Elbow.reconfigure :tight @19:49:30.89 Elbow.seek 1 @19:49:33.56 Forearm.reconfigure :tight @19:49:33.61 Forearm.seek 1 + 450 counts @19:49:34.57 Hand.open @19:49:35.31 SE.seek 1.82 Pucks below Top Plate @19:49:35.60 SE.reconfigure :seal @19:49:35.65 SE.seek 0.86 Pucks below Top Plate @19:49:36.24 Lights, camera, ACTION! @19:49:36.28 PuckCamera: snap -bin=2,2 -auto /var/log/esp/da24jan1719h50ml.tif @19:49:44.29 SX HX9/16 (USB:02): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 3.57 seconds /var/log/esp/da24jan1719h50ml.tif: TIFF Upload Complete @19:49:44.32 PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da24jan1719h50ml15s.tif @19:49:55.70 Waiting >20 seconds for da to relinquish FlushPuck @19:49:59.94 SX HX9/16 (USB:02): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15 seconds /var/log/esp/midres/da24jan1719h50ml15s.tif: TIFF Upload Complete @19:49:59.97 PuckCamera: snap -bin=2,2 20.0 /var/log/esp/midres/da24jan1719h50ml20s.tif @19:50:20.62 SX HX9/16 (USB:02): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 20 seconds /var/log/esp/midres/da24jan1719h50ml20s.tif: TIFF Upload Complete @19:50:20.63 Gate.power :camera,:OFF @19:50:20.65 DAfans.email "3.57 sec auto-exposure",Subject: "da24jan1719h50ml images!" @19:50:21.11 Elbow.seek 1 @19:50:21.34 Forearm.seek 1 + 450 counts @19:50:21.44 SE.reconfigure :gently @19:50:21.49 SE.seek 1.57 Pucks below Top Plate @19:50:22.16 Elbow.reconfigure :limp @19:50:22.24 Forearm.reconfigure :loose @19:50:22.28 Forearm.seek 1 @19:50:22.54 Elbow.hold @19:50:22.65 Elbow.reconfigure :tight @19:50:22.70 SE.reconfigure :default @19:50:22.75 SE.seek 1.76 Pucks below Top Plate @19:50:23.61 Forearm.force -20 @19:50:23.72 Forearm.seek 1 + 306 counts @19:50:24.29 Forearm.reconfigure :tight @19:50:24.44 Forearm.seek 1 + 450 counts @19:50:24.71 SE.seek :down @19:50:25.99 Forearm.reconfigure :loose @19:50:26.09 Forearm.seek :retract @19:50:27.32 SE.stop @19:50:27.53 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @19:50:27.93 Elbow.seek FlushPuck::Garage @19:50:29.89 Elbow.reconfigure :loose @19:50:30.09 Forearm.seek :garage @19:50:31.20 Hand.close @19:50:32.14 Forearm.seek :retract @19:50:33.22 Elbow.reconfigure :tight @19:50:33.33 Elbow.seek PC @19:50:34.71 Elbow.reconfigure :loose @19:50:34.93 Forearm.reconfigure :tight @19:50:35.06 Forearm.seek PC @19:50:36.72 PC.seek :closed @19:50:41.46 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.63 @19:50:41.53 Hand.open @19:50:41.78 upsync @19:50:42.18 Forearm.reconfigure :loose @19:50:42.23 Forearm.seek :retract @19:50:43.19 PSR.select :air @19:50:43.21 PSV.dial PSR @19:50:43.68 Pulling 0.5ml of PSR.flush @19:50:43.69 PSR.select :flush @19:50:43.81 PS.seek 0.5ml @19:50:49.77 delay 5 seconds @19:50:54.79 PSR.select :air @19:50:54.91 PS.seek 2.5ml @19:51:16.24 delay 1 second @19:51:17.26 PRV.dial :waste2,avoiding: :ab2 @19:51:19.10 PTV.dial :PRVmixing @19:51:20.39 PSV.dial :mixloop @19:51:21.54 PS.seek :empty @19:51:47.92 PSR.select :air @19:51:47.94 PSV.dial PSR @19:51:48.97 Pulling 0.5ml of PSR.flush @19:51:48.98 PSR.select :flush @19:51:49.10 PS.seek 0.5ml @19:51:55.08 delay 5 seconds @19:52:00.10 PSR.select :air @19:52:00.22 PS.seek 2.5ml @19:52:21.49 delay 1 second @19:52:22.51 PBV.dial :waste1 @19:52:22.88 PTV.dial :mixing @19:52:23.63 PSV.dial :mixloop @19:52:24.68 PS.seek :empty @19:52:51.02 PSV.dial PSR @19:52:52.06 PSR.select :flush @19:52:52.19 PS.seek 1ml @19:53:03.27 PSR.select :air @19:53:03.38 PS.seek 2.5ml @19:53:19.55 PSV.dial PTV @19:53:20.31 PTV.dial :puck @19:53:21.05 PBV.dial :waste1 @19:53:21.24 PS.seek 0ml @19:53:47.58 PSV.dial PSR @19:53:48.33 PSR.select :flush @19:53:48.45 PS.seek 0.5ml @19:53:54.33 PSR.select :air @19:53:54.45 PS.seek 2.5ml @19:54:15.67 PSV.dial PTV @19:54:16.42 PBV.dial PSV @19:54:16.97 PS.seek 0ml @19:54:43.33 PSV.dial PBV @19:54:43.80 PS.seek 2.5ml @19:55:10.11 PSV.dial :waste1 @19:55:10.85 PS.seek 0ml @19:55:37.36 PSV.dial PSR @19:55:37.83 PSR.select :air @19:55:37.85 PS.seek 2.5ml @19:56:04.30 PSV.dial PTV @19:56:05.03 PBV.dial PSV @19:56:05.22 PS.seek 0ml @19:56:31.57 PSV.dial PBV @19:56:32.05 PS.seek 2.5ml @19:56:58.39 PSV.dial :waste1 @19:56:59.13 PS.seek 0ml @19:57:25.54 PSV.dial PSR @19:57:26.01 PSR.select :flush @19:57:26.13 PS.seek 1ml @19:57:37.13 PSR.select :air @19:57:37.25 PS.seek 2.5ml @19:57:53.48 PSV.dial PTV @19:57:54.21 PTV.dial :puck @19:57:54.29 PBV.dial :waste1 @19:57:54.85 PS.seek 0ml @19:58:21.26 PSV.dial PSR @19:58:22.01 PSR.select :flush @19:58:22.13 PS.seek 0.5ml @19:58:28.04 PSR.select :air @19:58:28.16 PS.seek 2.5ml @19:58:49.42 PSV.dial PTV @19:58:50.17 PBV.dial PSV @19:58:50.73 PS.seek 0ml @19:59:17.07 PSV.dial PBV @19:59:17.55 PS.seek 2.5ml @19:59:43.85 PSV.dial :waste1 @19:59:44.59 PS.seek 0ml @20:00:10.98 PSV.dial PSR @20:00:11.45 PSR.select :air @20:00:11.47 PS.seek 2.5ml @20:00:37.91 PSV.dial PTV @20:00:38.65 PBV.dial PSV @20:00:38.84 PS.seek 0ml @20:01:05.38 PSV.dial PBV @20:01:05.86 PS.seek 2.5ml @20:01:32.19 PSV.dial :waste1 @20:01:32.93 PS.seek 0ml @20:01:59.32 Garaging FlushPuck in Processing Clamp @20:01:59.39 Elbow.reconfigure :tight @20:01:59.44 Elbow.seek PC @20:01:59.80 Elbow.reconfigure :loose @20:02:00.35 Forearm.seek PC @20:02:01.31 Hand.close @20:02:01.99 PC.seek :open @20:02:06.71 Forearm.seek :retract @20:02:07.67 Elbow.reconfigure :tight @20:02:07.72 Elbow.seek :garage @20:02:09.02 Elbow.reconfigure :loose @20:02:09.13 Forearm.reconfigure :tight @20:02:09.17 Forearm.seek :garage @20:02:10.22 Hand.open @20:02:10.92 Forearm.reconfigure :loose @20:02:10.97 Forearm.seek :retract @20:04:52.71 Session Begun @20:04:55.69 -> CC Collection Clamp contains wcr puck from tube 2 @20:05:12.73 -> CC.unloadPuck 1, :WCR @20:05:12.82 Puck::Warning in dave -- Unloading Puck.from 2, :wcr to Storage Carousel @20:05:12.84 Storing WCR puck in Collection Clamp to tube 1 @20:05:13.27 Elbow.reconfigure :tight @20:05:13.34 Elbow.seek 1 @20:05:15.24 Forearm.reconfigure :tight @20:05:15.31 Forearm.seek 1 + 450 counts @20:05:16.34 SE.reconfigure :pick @20:05:16.42 SE.seek :beamBreak @20:05:19.40 SE.configure :default @20:05:19.58 Elbow.reconfigure :limp @20:05:19.65 Forearm.reconfigure :loose @20:05:19.72 Forearm.seek 1 @20:05:19.98 Elbow.hold @20:05:20.07 Elbow.reconfigure :tight @20:05:20.19 SE.seek 2.16 Pucks below Top Plate @20:05:20.65 Forearm.force -20 @20:05:20.70 Forearm.seek 1 + 296 counts @20:05:21.00 Forearm.reconfigure :tight @20:05:21.08 Forearm.seek 1 + 450 counts @20:05:21.28 SE.seek 3.16 Pucks below Top Plate @20:05:21.86 Forearm.reconfigure :loose @20:05:21.92 Forearm.seek :retract @20:05:22.93 Elbow.seek CC @20:05:24.58 Elbow.reconfigure :loose @20:05:24.70 Forearm.seek CC @20:05:25.76 Hand.close @20:05:26.36 CC.seek :open @20:05:31.11 Forearm.seek :retract @20:05:32.11 Elbow.reconfigure :tight @20:05:32.18 Elbow.seek 1 @20:05:33.95 Elbow.reconfigure :limp @20:05:34.02 Forearm.seek 1 @20:05:35.03 Elbow.hold @20:05:35.11 Elbow.reconfigure :tight @20:05:35.18 Hand.open @20:05:35.98 Forearm.force -20 @20:05:36.04 Forearm.seek 1 + 290 counts @20:05:36.35 Forearm.reconfigure :tight @20:05:36.42 Forearm.seek 1 + 450 counts @20:05:36.70 SE.seek :down @20:05:37.84 Forearm.reconfigure :loose @20:05:37.91 Forearm.seek :retract @20:05:39.24 SE.stop @20:05:47.36 -> CC Collection Clamp open @20:05:49.11 -> PC Processing Clamp open @20:05:51.81 -> Flush FlushPuck is in FlushPuck::Garage @20:05:54.26 -> access 1 @20:05:54.47 SC.reconfigure :tight @20:05:54.54 SC.seek 5 @20:06:07.58 SC.configure :limp Storage Carousel at 5 @20:06:51.65 -> CSV.CSVflush @20:06:51.67 NoMethodError in dave -- undefined method `CSVflush' for CSV:Shaft @20:07:12.14 -> CSV.flush @20:07:12.15 CSV.dial :flush Collection Syringe Valve at flush @20:07:23.62 -> CS.fill @20:07:23.64 CS.seek 10ml Collection Syringe at 10ml @20:08:00.79 -> CSV.waste1 @20:08:00.81 CSV.dial :waste1 Collection Syringe Valve at waste1 @20:08:15.70 -> CS.empty @20:08:15.72 CS.seek 0ml Collection Syringe at 0ml @20:08:49.43 -> CBV.waste1 @20:08:49.45 CBV.dial :waste1 Collection Bottom Valve at waste1 @20:09:03.48 -> CTV.CSV @20:09:03.49 CTV.dial CSV Collection Top Valve at CSV @20:09:10.43 -> Flush.CC @20:09:10.45 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @20:09:10.65 Elbow.seek FlushPuck::Garage @20:09:12.54 Elbow.reconfigure :loose @20:09:12.66 Forearm.seek :garage @20:09:13.63 Hand.close @20:09:14.34 Forearm.seek :retract @20:09:15.34 Elbow.reconfigure :tight @20:09:15.41 Elbow.seek CC @20:09:18.30 Elbow.reconfigure :loose @20:09:18.42 Forearm.reconfigure :tight @20:09:18.49 Forearm.seek CC @20:09:19.73 CC.seek :closed @20:09:24.26 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.29 @20:09:24.34 Hand.open @20:09:25.04 Forearm.reconfigure :loose @20:09:25.10 Forearm.seek :retract FlushPuck::Garage @20:09:32.16 -> CS.flush @20:09:32.18 NoMethodError in dave -- undefined method `flush' for CS:Syringe @20:09:41.39 -> CSV.flush @20:09:41.41 CSV.dial :flush Collection Syringe Valve at flush @20:09:48.17 -> CS.fill @20:09:48.19 CS.seek 10ml Collection Syringe at 10ml @20:10:21.18 -> CSV.CTV @20:10:21.19 CSV.dial CTV Collection Syringe Valve at CTV @20:10:26.03 -> CTV.CBV @20:10:26.05 NoMethodError in dave -- undefined method `CBV' for CTV:Shaft @20:10:35.86 -> CTV.CSV @20:10:35.88 CTV.dial CSV Collection Top Valve at CSV @20:10:41.97 -> CS.empty @20:10:41.99 CS.seek 0ml Collection Syringe at 0.001ml @20:14:12.33 -> access 2 @20:14:12.54 SC.reconfigure :tight @20:14:12.60 SC.seek 6 @20:14:16.17 SC.configure :limp Storage Carousel at 6 @20:14:32.91 -> Flush.garage @20:14:32.93 Garaging FlushPuck in Collection Clamp @20:14:33.02 Elbow.reconfigure :tight @20:14:33.09 Elbow.seek CC @20:14:33.41 Elbow.reconfigure :loose @20:14:33.52 Forearm.seek CC @20:14:34.61 Hand.close @20:14:35.23 CC.seek :open @20:14:39.89 Forearm.seek :retract @20:14:41.44 Elbow.reconfigure :tight @20:14:41.51 Elbow.seek :garage @20:14:44.47 Elbow.reconfigure :loose @20:14:44.59 Forearm.reconfigure :tight @20:14:44.66 Forearm.seek :garage @20:14:45.63 Hand.open @20:14:46.28 Forearm.reconfigure :loose @20:14:46.35 Forearm.seek :retract FlushPuck::Garage @20:14:50.85 -> Flush FlushPuck is in FlushPuck::Garage @20:14:52.20 -> CC Collection Clamp open @20:14:53.59 -> PC Processing Clamp open @20:15:43.66 -> exit @20:15:43.67 Session Ended + ESPwhich DA_WCR_011024.rb + fn=DA_WCR_011024.rb + IFS=: + set /home/esp/esp2/mission . + fullPath=/home/esp/esp2/mission/DA_WCR_011024.rb + [ -f /home/esp/esp2/mission/DA_WCR_011024.rb ] + realpath /home/esp/esp2/mission/DA_WCR_011024.rb + return 0 + scriptPath=/home/esp/esp2local/mission/DA_WCR_011024.rb + exec ruby /home/esp/esp2/lib/esp.rb /home/esp/esp2local/mission/DA_WCR_011024.rb @14:09:02.37UTC18-Jan-24
ESP2 v3.42 I2C Gateway v3.20 for core bus @14:09:05.15 Configuration for short puck jake 1/11/21 by brent@mbari.org /home/esp/esp2/type/shallow/jake/configure.rb @14:09:08.96 Polling Can every 10:00 @14:09:08.98 Cleanly exited at 14:08:41UTC18-Jan-24 @14:09:09.03 Loading /home/esp/esp2local/mission/DA_WCR_011024.rb mission @14:09:09.09 GoodNews.email "Starting DA_WCR_011024 w/pucks in tube 2\n{3 => 0,\n 4 => 0,\n 5 => 0,\n 6 => 0,\n 7 => 0}\nCTD data unavailable\nCan@14:09:08, 26% flow, 22.1C, 10% humidity, 15.0psia, 13.788V, 0.369A, 56.831Ah, 5.09W",Subject: "DA_WCR_011024 begun" @14:09:09.12
Preparing to sleep until + 00:00:05 @14:09:09.18 Asleep til + 00:00:05 @14:09:09.19 Continuing at 14:09 @14:09:09.48 Gate.power :core,:OFF @14:09:09.49 Gate.power :raw,:OFF @14:09:09.50 Shutdown @14:09:11.90 GoodNews.email "Then, in tube 2: initialPurge; da [50, 50] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_011024.rb:14\n{1 => 0,\n 2 => 4,\n 3 => 0,\n 4 => 0,\n 5 => 0,\n 6 => 0,\n 7 => 0}\nCTD data unavailable\nCan@14:09:09, 26% flow, 22.1C, 10% humidity, 15.0psia, 13.788V, 0.381A, 56.831Ah, 5.25W",Subject: "Asleep until 14:09" @14:09:13.47 upsync @14:09:14.07
Powering core bus @14:09:15.77 upsync @14:09:19.42
Gate.power :raw,:ON @14:09:20.48 CS.configure :default @14:09:20.53 PS.configure :default @14:09:20.58 SS.configure :default @14:09:20.63 CC.configure :default @14:09:20.68 PC.configure :default @14:09:20.74 Forearm.configure :loose @14:09:20.79 Elbow.configure :loose @14:09:20.86 SC.configure :loose @14:09:20.91 SE.configure :default @14:09:20.96 CH.configure :default @14:09:21.00 PH.configure :default @14:09:21.04 All dwarf controllers running firmware version 3.85 @14:09:21.15 Valve.reset @14:09:21.42 Intake.select :close @14:09:21.46 Exhaust.select :close @14:09:21.47 CTV.dial :air @14:09:21.54 PSV.dial :air @14:09:21.66
Forearm.home.seek :retract @14:09:22.40 Hand.close @14:09:22.43
Elbow.reconfigure :loose @14:09:22.53 Elbow.jog 1500 @14:09:22.64 PS.home.seek 0ml @14:09:22.79 CSV.dial :waste1 @14:09:23.06
Elbow.reconfigure :loose @14:09:23.11 Elbow.home.seek :clear @14:09:23.79 SSV.dial :spit,avoiding: :exhaust @14:09:24.66 CS.reconfigure :fast @14:09:24.68 SS.home.seek 0ml @14:09:24.78 CS.home.seek 0ml @14:09:26.33
SE.home.seek :down @14:09:26.96 SC.reconfigure :tight @14:09:27.01 SC.home.seek :initial @14:09:31.07 PS.stop @14:09:52.19
SC.configure :limp @14:09:52.25 Forearm.seek :retract @14:09:52.31 Elbow.seek :hammer @14:09:53.44 Forearm.reconfigure :tight @14:09:53.48 Forearm.seek retracted @14:09:53.76 PC.home.seek :open @14:09:53.80
CC.home.seek :open @14:09:57.23 Phase [initialPurge; da [50, 50] #da and wsr are run simultanelously] @14:09:57.24 Notice.email "At 14:09:57 began in tube 2: initialPurge; da [50, 50] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_011024.rb:14\n{1 => 0,\n 2 => 4,\n 3 => 0,\n 4 => 0,\n 5 => 0,\n 6 => 0,\n 7 => 0}\nCTD data unavailable\nCan@14:09:57, 25% flow, 22.2C, 10% humidity, 15.0psia, 13.778V, 0.333A, 56.836Ah, 4.58W",Subject: "Started DA_WCR_011024 phase initialPurge; da [50, 50] #da and wsr are run simultanelously" @14:09:57.26
Starting initialPurge @14:09:57.29 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:09:57.36 Hand.open @14:09:58.13 Elbow.reconfigure :tight @14:09:58.18 Elbow.seek FlushPuck::Garage @14:10:00.17 Elbow.reconfigure :loose @14:10:00.30 Forearm.reconfigure :loose @14:10:00.35 Forearm.seek :garage @14:10:01.25 upsync @14:10:01.37
Hand.close @14:10:02.14 Forearm.seek :retract @14:10:03.17 Elbow.reconfigure :tight @14:10:03.22 Elbow.seek CC @14:10:06.16 Elbow.reconfigure :loose @14:10:06.26 Forearm.reconfigure :tight @14:10:06.31 Forearm.seek CC @14:10:07.55 CC.seek :closed @14:10:12.14 Collection Clamp onSpring/offSpring for 14/27 samples, on/off torque 2.27 @14:10:12.21 Hand.open @14:10:12.95 Forearm.reconfigure :loose @14:10:13.01 Forearm.seek :retract @14:10:13.97 Flushing the Collection Valve::Manifold @14:10:14.09 CSV.dial CSR @14:10:15.37 Pulling 5ml of CSR.flush @14:10:15.38 CSR.select :flush @14:10:15.50 CS.reconfigure :default @14:10:15.54 CS.seek 5ml @14:10:29.40 delay 5 seconds @14:10:34.42 CSR.select :air @14:10:35.00 CS.reconfigure :fast @14:10:35.05 CS.seek 7.5ml @14:10:41.83 CSV.dial :waste @14:10:43.12 CS.reconfigure :default @14:10:43.17 CS.seek 0ml @14:11:03.27 Purging external Intake @14:11:03.39 CSV.dial :flush @14:11:03.86 CS.reconfigure :fast @14:11:03.90 CS.seek 10ml @14:11:28.80 delay 5 seconds @14:11:33.83 CSV.dial CBV @14:11:33.86
CBV.dial CSV @14:11:34.65 CTV.dial :intake @14:11:35.41 Intake.select :open @14:11:35.52 CS.seek 0ml @14:11:59.43 Intake.select :close @14:11:59.53 CTV.dial :air @14:12:00.29 Flushing upper manifold @14:12:00.41 CSV.dial :flush @14:12:01.15 CS.seek 5ml @14:12:14.15 delay 5 seconds @14:12:19.16 CSV.dial :air @14:12:19.66 CS.seek 7ml @14:12:25.27 CSV.dial CBV @14:12:25.30
CBV.dial CSV @14:12:26.32 CTV.dial :air @14:12:26.34 CS.seek 0ml @14:12:43.02 delay 7 seconds @14:12:50.04 CS.seek 3ml @14:12:58.11 CSV.dial :waste @14:12:58.57 CS.seek 0ml @14:13:06.63 CSV.dial :flush @14:13:07.07 CS.seek 3ml @14:13:15.33 delay 5 seconds @14:13:20.35 CSV.dial :air @14:13:20.86 CS.seek 5.001ml @14:13:26.67 CSV.dial :air @14:13:26.76 CS.seek 0ml @14:13:39.18 delay 7 seconds @14:13:46.20 CS.seek 3ml @14:13:54.33 CSV.dial :waste @14:13:55.07 CS.seek 0ml @14:14:02.77 Garaging FlushPuck in Collection Clamp @14:14:02.84 Elbow.reconfigure :tight @14:14:02.89 Elbow.seek CC @14:14:03.13 Elbow.reconfigure :loose @14:14:03.23 Forearm.seek CC @14:14:04.18 Hand.close @14:14:04.76 CC.seek :open @14:14:09.75 Forearm.seek :retract @14:14:10.79 Elbow.reconfigure :tight @14:14:10.84 Elbow.seek :garage @14:14:13.67 Elbow.reconfigure :loose @14:14:13.77 Forearm.reconfigure :tight @14:14:13.82 Forearm.seek :garage @14:14:14.88 Hand.open @14:14:15.57 Forearm.reconfigure :loose @14:14:15.61 Forearm.seek :retract @14:14:16.63 da sampling at most 50ml, wcr at most 50ml @14:14:16.67 DAfans.email "Started da in tube 2 at 14:14:16UTC18-Jan-24\n{1 => 0,\n 2 => 4,\n 3 => 0,\n 4 => 0,\n 5 => 0,\n 6 => 0,\n 7 => 0}\nCTD data unavailable\nCan@14:09:57, 25% flow, 22.2C, 10% humidity, 15.0psia, 13.778V, 0.333A, 56.836Ah, 4.58W",Subject: "da sampling at most 50ml, wcr at most 50ml" @14:14:16.70
Loading sh1 puck from tube 2 into Collection Clamp @14:14:16.97 SC.reconfigure :tight @14:14:17.02 SC.seek 2 @14:14:20.56 upsync @14:14:23.75
SC.configure :limp @14:14:23.99 Elbow.reconfigure :tight @14:14:24.04 Elbow.seek 2 @14:14:25.92 Forearm.reconfigure :tight @14:14:25.97 Forearm.seek 2 + 450 counts @14:14:26.98 SE.reconfigure :pick @14:14:27.03 SE.seek :beamBreak @14:14:29.98 SE.configure :default @14:14:30.15 Elbow.seek 2 @14:14:30.34 Forearm.seek 2 + 300 counts @14:14:30.72 Forearm.reconfigure :loose @14:14:30.77 Hand.close @14:14:31.40 SE.seek :down @14:14:31.61
Forearm.seek :retract @14:14:32.55 Elbow.seek CC @14:14:33.98 SE.stop @14:14:34.66
Elbow.reconfigure :loose @14:14:34.76 Forearm.reconfigure :tight @14:14:34.81 Forearm.seek CC @14:14:36.11 CC.seek :closed @14:14:40.68 Collection Clamp onSpring/offSpring for 14/27 samples, on/off torque 2.24 @14:14:40.69 Hand.open @14:14:41.32 Forearm.reconfigure :loose @14:14:41.36 Forearm.seek :retract @14:14:42.37 collectSample 50ml w/bubble point of 24psi @14:14:42.38 Priming 7ml @14:14:42.39 Intake.select :close @14:14:42.40 Exhaust.select :close @14:14:42.41 SS.hold @14:14:42.62 Calibrating pressure and temperature sensors @14:14:42.65 CTV.dial :air @14:14:42.66 SSV.dial :spit,avoiding: :exhaust @14:14:42.85 SS.reconfigure :fast @14:14:42.90 SS.seek 5ml @14:14:45.83 SSV.dial CBV @14:14:45.86
CBV.dial SSV @14:14:47.21 delay 3.8 seconds @14:14:51.13 Sensor limits: Intake max 118.2psia; Outlet max 111.4psia @14:14:51.14 SSV.dial :spit @14:14:52.44 SS.seek 0ml @14:14:55.12 SSV.dial CBV @14:14:56.45 CTV.dial :intake @14:14:57.24 SSV.dial CBV @14:14:57.28
CBV.dial SSV @14:14:57.46 Allowing 40s for 7.0ml of remaining 7.0ml @14:14:57.47 Intake.select :open @14:14:59.63 Intake @15.0psia, vacuum from 9.5 to 12.5psi @14:14:59.65 SS.reconfigure :inhaling 2.20ml/s @14:14:59.70 SS.seek 7ml @14:15:06.43 Stroke completed -- waiting for pressure to equalize ... @14:15:06.44 SS.reconfigure :exhale @14:15:06.49 SS.hold @14:15:09.56 Intake.select :close @14:15:09.71 SSV.dial :exhaust @14:15:10.47 Exhaust.select :open @14:15:10.58 SS.seek 0ml @14:15:14.58 Exhaust.select :close @14:15:14.69 Sampling 50.0ml @14:15:14.70 Intake.select :close @14:15:14.71 Exhaust.select :close @14:15:14.72 SS.reconfigure :default @14:15:14.77 SS.hold @14:15:14.97 CTV.dial :intake @14:15:15.16 SSV.dial CBV @14:15:15.19
CBV.dial SSV @14:15:15.94 Allowing 130s for 25.0ml of remaining 50.0ml @14:15:15.95 Intake.select :open @14:15:18.11 Intake @15.5psia, vacuum from 10.0 to 13.0psi @14:15:18.13 SS.reconfigure :inhaling 2.20ml/s @14:15:18.18 SS.seek 25ml @14:15:32.33 Vacuum too high -- waiting up to 40 seconds @14:15:32.35 SS.reconfigure :exhale @14:15:32.40 SS.hold @14:15:33.10 Intake @12.3psia, vacuum from 6.8 to 9.8psi @14:15:33.12 SS.reconfigure :inhaling 1.76ml/s @14:15:33.17 SS.seek 25ml @14:15:34.50 Stroke completed -- waiting for pressure to equalize ... @14:15:34.51 SS.reconfigure :exhale @14:15:34.55 SS.hold @14:15:40.19 Intake.select :close @14:15:40.34 SSV.dial :exhaust @14:15:41.09 Exhaust.select :open @14:15:41.21 SS.seek 0ml @14:15:50.03 Exhaust.select :close @14:15:50.14 SSV.dial CBV @14:15:50.16
CBV.dial SSV @14:15:50.94 Allowing 130s for 25.0ml of remaining 25.0ml @14:15:50.95 Intake.select :open @14:15:53.46 Intake @15.7psia, vacuum from 10.0 to 13.0psi @14:15:53.55 SS.reconfigure :inhaling 1.76ml/s @14:15:53.60 SS.seek 25ml @14:16:04.61 Vacuum too high -- waiting up to 40 seconds @14:16:04.63 SS.reconfigure :exhale @14:16:04.68 SS.hold @14:16:07.40 Intake @13.6psia, vacuum from 8.1 to 11.1psi @14:16:07.42 SS.reconfigure :inhaling 1.41ml/s @14:16:07.47 SS.seek 25ml @14:16:16.28 Stroke completed -- waiting for pressure to equalize ... @14:16:16.29 SS.reconfigure :exhale @14:16:16.33 SS.hold @14:16:24.88 Intake.select :close @14:16:25.04 SSV.dial :exhaust @14:16:25.78 Exhaust.select :open @14:16:25.89 SS.seek 0ml @14:16:34.73 Exhaust.select :close @14:16:34.84 Sampled 50.0ml @14:16:34.85 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @14:16:34.86 Intake.select :close @14:16:34.92 CSV.dial :waste @14:16:35.04 SSV.dial CBV,avoiding: :exhaust @14:16:35.07
CBV.dial SSV @14:16:35.15 CS.seek 0ml @14:16:35.58 CSV.dial :air @14:16:36.37 CS.seek 10ml @14:16:36.97
CTV.dial :air @14:16:37.72 delay 15 seconds @14:16:52.74 SSV.dial :spit,avoiding: :exhaust @14:16:54.07 CTV.dial CSV,avoiding: :intake @14:16:54.10
SS.seek 0ml @14:16:54.23 SSV.dial CBV,avoiding: :exhaust @14:17:01.05 SSV.dial between CBV and plug @14:17:01.53 CBV.dial between SSV and waste2 @14:17:01.92 CTV.dial CSV,avoiding: :air @14:17:01.95
CSV.dial CTV @14:17:02.47 CS.reconfigure :pressurize @14:17:02.52 CS.seek 0ml @14:17:21.17 BubblePoint: Intake @24.2psia, Outlet @0.7psia @14:17:21.18 CBV.dial SSV @14:17:21.52 SS.reconfigure :default @14:17:21.57 SS.seek 10ml @14:17:27.20 delay 15 seconds @14:17:42.33 Evacuated: Intake @23.9psia, Outlet @24.2psia @14:17:42.34 SSV.dial :spit,avoiding: :exhaust @14:17:43.38 SS.reconfigure :exhale @14:17:43.42 SS.seek 0ml @14:17:48.43 SS.reconfigure :fast @14:17:48.48 SS.seek 5ml @14:17:51.33 SSV.dial CBV,avoiding: :exhaust @14:17:51.36
CBV.dial SSV @14:17:52.69 CTV.dial :air,avoiding: :intake @14:17:53.97 CSV.dial CTV @14:17:54.01
SS.seek 10ml @14:17:55.13 CSV.dial :air @14:17:55.58 CS.reconfigure :fast @14:17:55.63 CS.seek 0ml @14:17:56.53
SSV.dial :spit,avoiding: :exhaust @14:17:57.84 SS.reconfigure :exhale @14:17:57.89 SS.seek 0ml @14:18:03.17 CS.stop @14:18:03.22
SS.reconfigure :fast @14:18:03.27 SS.seek 5ml @14:18:06.14 SSV.dial CBV,avoiding: :exhaust @14:18:06.17
CBV.dial SSV @14:18:08.51 SSV.dial :spit,avoiding: :exhaust @14:18:09.83 SS.reconfigure :default @14:18:09.88 SS.seek 0ml @14:18:13.18 SS.stop @14:18:13.23 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @14:18:13.24 Intake.select :close @14:18:13.31 CSV.dial :waste @14:18:13.44 SSV.dial CBV,avoiding: :exhaust @14:18:13.46
CBV.dial SSV @14:18:14.07 CS.seek 0ml @14:18:14.73 CSV.dial :air @14:18:14.80
CTV.dial :air @14:18:14.87 delay 15 seconds @14:18:15.58 CS.seek 10ml @14:18:29.89
SSV.dial :spit,avoiding: :exhaust @14:18:31.20 CTV.dial CSV,avoiding: :intake @14:18:31.24
SS.reconfigure :exhale @14:18:31.34 SS.seek 0ml @14:18:31.76 SSV.dial CBV,avoiding: :exhaust @14:18:39.99 SSV.dial between CBV and plug @14:18:40.45 CBV.dial between SSV and waste2 @14:18:40.84 CSV.dial between air and CTV @14:18:41.29 CS.seek 8.17ml @14:18:46.36 CTV.dial CSV,avoiding: :air @14:18:46.39
CSV.dial CTV @14:18:46.91 CS.reconfigure :pressurize @14:18:46.96 CS.seek 0ml @14:19:04.95 BubblePoint: Intake @24.2psia, Outlet @1.8psia @14:19:04.96 CBV.dial SSV @14:19:05.30 SS.reconfigure :default @14:19:05.35 SS.seek 10ml @14:19:11.09 delay 15 seconds @14:19:26.22 Evacuated: Intake @24.0psia, Outlet @16.1psia @14:19:26.23 SSV.dial :spit,avoiding: :exhaust @14:19:27.28 SS.reconfigure :exhale @14:19:27.33 SS.seek 0ml @14:19:32.33 SS.reconfigure :fast @14:19:32.38 SS.seek 5ml @14:19:35.19 SSV.dial CBV,avoiding: :exhaust @14:19:35.21
CBV.dial SSV @14:19:36.56 CTV.dial :air,avoiding: :intake @14:19:37.86 CSV.dial CTV @14:19:37.91
SS.seek 10ml @14:19:39.02 CSV.dial :air @14:19:39.45 CS.reconfigure :fast @14:19:39.50 CS.seek 0ml @14:19:40.40
SSV.dial :spit,avoiding: :exhaust @14:19:41.73 SS.reconfigure :exhale @14:19:41.78 SS.seek 0ml @14:19:43.45 CS.stop @14:19:47.13
SS.reconfigure :fast @14:19:47.18 SS.seek 5ml @14:19:49.95 SSV.dial CBV,avoiding: :exhaust @14:19:49.98
CBV.dial SSV @14:19:52.33 SSV.dial :spit,avoiding: :exhaust @14:19:53.64 SS.reconfigure :default @14:19:53.69 SS.seek 0ml @14:19:56.94 SS.stop @14:19:56.98 Lysing: Adding 2.0ml AqMeOH @14:19:57.00 CBV.dial between CSV and waste1,avoiding: :DBV @14:19:58.84 CTV.dial CSV @14:20:00.18 CSV.dial CSR @14:20:00.97 Pulling 1ml of CSR.AqMeOH @14:20:00.98 CSR.select :AqMeOH @14:20:01.10 CS.reconfigure :default @14:20:01.15 CS.seek 1ml @14:20:04.51 delay 5 seconds @14:20:09.53 CSR.select :air @14:20:09.70 CS.seek 6ml @14:20:23.26 delay 2 seconds @14:20:25.28 CSV.dial :waste2 @14:20:25.72 CS.reconfigure :fast @14:20:25.77 CS.seek 0ml @14:20:40.20 CSV.dial CSR @14:20:40.65 Pulling 2ml of CSR.AqMeOH @14:20:40.66 CSR.select :AqMeOH @14:20:40.78 CS.reconfigure :default @14:20:40.82 CS.seek 2ml @14:20:47.01 delay 5 seconds @14:20:52.03 CSR.select :air @14:20:52.20 CS.seek 5ml @14:21:00.89 delay 2 seconds @14:21:02.90 CSV.dial CTV @14:21:03.35 CS.seek 1ml @14:21:14.74 Before lysing Collection Puck Heater at 21.4C/25.3psia @14:21:14.76 Heating Puck.from 2, :sh1 in Collection Clamp to 70C for 10:00 @14:21:14.78 CH.seek 70C,8:00 @14:23:15.15 CH.hold 10:00,70C @14:23:15.35 Begun lysing Collection Puck Heater at 68.1C/30.3psia @14:33:15.36 Ended lysing Collection Puck Heater at 70C/29.8psia @14:33:15.38 CS.seek 0ml @14:33:19.00 CTV.dial between spare and air,avoiding: :intake @14:33:20.03 CSV.dial :air @14:33:20.48 CS.seek 5ml @14:33:34.28 CSV.dial CTV @14:33:34.73 delay 2 seconds @14:33:36.75 CSV.dial :air @14:33:37.20 delay 2 seconds @14:33:39.22 CSV.dial CTV @14:33:39.67 delay 2 seconds @14:33:41.69 CSV.dial :air @14:33:42.14 delay 2 seconds @14:33:44.21 CS.seek 0ml @14:33:58.11 Puck @59.2psia after final lysis pressurization @14:33:58.12 Recovering Lysate into Collection syringe @14:33:58.13 CSV.dial CBV @14:33:59.12 CBV.dial CSV @14:33:59.50 CS.seek 5.001ml @14:34:13.06 delay 5 seconds @14:34:18.08 CTV.dial :air @14:34:18.60 CS.seek 7ml @14:34:24.50 delay 5 seconds @14:34:29.52 CSV.dial :air2 @14:34:29.96 CS.seek 10ml @14:34:38.53 CSV.dial between air and CTV,avoiding: CBV @14:34:39.69 Lysing Complete @14:34:39.76 Storing sh1 puck in Collection Clamp to tube 1 @14:34:40.38 SC.reconfigure :tight @14:34:40.43 SC.seek 1 @14:34:44.04 SC.configure :limp @14:34:44.17 SE.reconfigure :place @14:34:44.27
Elbow.reconfigure :tight @14:34:44.31 SE.seek :beamBreak @14:34:44.34
Elbow.seek CC @14:34:44.66 Elbow.reconfigure :loose @14:34:44.76 Forearm.seek CC @14:34:45.75 Hand.close @14:34:46.37 CC.seek :open @14:34:47.19 SE.configure :default @14:34:47.24 SE.seek :place @14:34:51.11
Forearm.seek :retract @14:34:52.08 Elbow.reconfigure :tight @14:34:52.13 Elbow.seek 1 @14:34:54.04 Elbow.reconfigure :limp @14:34:54.09 Forearm.seek 1 @14:34:55.05 Elbow.hold @14:34:55.11 Elbow.reconfigure :tight @14:34:55.16 Hand.open @14:34:55.86 Forearm.force -20 @14:34:55.91 Forearm.seek 1 + 305 counts @14:34:56.21 Forearm.reconfigure :tight @14:34:56.26 Forearm.seek 1 + 450 counts @14:34:56.51 SE.seek :down @14:34:57.64
Forearm.reconfigure :loose @14:34:57.69 Forearm.seek :retract @14:34:59.20 SE.stop @14:34:59.24
Tranferring DA @14:34:59.25 Beginning samplePassC @14:34:59.26 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:34:59.45 Elbow.seek FlushPuck::Garage @14:35:01.69 Elbow.reconfigure :loose @14:35:01.79 Forearm.seek :garage @14:35:02.80 Hand.close @14:35:03.48 Forearm.seek :retract @14:35:04.47 Elbow.reconfigure :tight @14:35:04.52 Elbow.seek CC @14:35:07.39 Elbow.reconfigure :loose @14:35:07.49 Forearm.reconfigure :tight @14:35:07.54 Forearm.seek CC @14:35:08.88 CC.seek :closed @14:35:13.41 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.33 @14:35:13.48 Hand.open @14:35:14.18 Forearm.reconfigure :loose @14:35:14.22 Forearm.seek :retract @14:35:15.19 PSV.dial PSR @14:35:16.67 Pulling 1.5ml of PSR.flush @14:35:16.68 PSR.select :flush @14:35:16.80 PS.seek 1.5ml @14:35:32.92 delay 5 seconds @14:35:37.93 PSV.dial :waste1 @14:35:38.42 PS.seek 0ml @14:35:54.73 PSV.dial PSR @14:35:55.19 PSR.select :air @14:35:55.31 PS.seek 2.5ml @14:36:21.76 PRV.dial CBV,avoiding: :ab2 @14:36:21.79
CBV.dial PRV @14:36:23.51 PSV.dial :mixloop @14:36:24.65 CTV.dial :air,avoiding: :intake @14:36:24.89 PTV.dial :PRVmixing @14:36:26.27 PS.seek 0ml @14:36:52.66 PSV.dial :air @14:36:53.14 PS.seek 2.5ml @14:37:19.57 PSV.dial :mixloop @14:37:20.04 PS.seek 0ml @14:37:46.35 PSV.dial :air @14:37:46.93 PS.seek 2.5ml @14:38:13.32 PSV.dial :mixloop @14:38:13.78 PS.seek 0ml @14:38:40.19 Garaging FlushPuck in Collection Clamp @14:38:40.26 Elbow.reconfigure :tight @14:38:40.31 Elbow.seek CC @14:38:40.63 Elbow.reconfigure :loose @14:38:40.73 Forearm.seek CC @14:38:41.78 Hand.close @14:38:42.36 CC.seek :open @14:38:46.98 Forearm.seek :retract @14:38:48.02 Elbow.reconfigure :tight @14:38:48.07 Elbow.seek :garage @14:38:50.93 Elbow.reconfigure :loose @14:38:51.03 Forearm.reconfigure :tight @14:38:51.08 Forearm.seek :garage @14:38:52.15 Hand.open @14:38:52.79 Forearm.reconfigure :loose @14:38:52.84 Forearm.seek :retract @14:38:53.83 PSV.dial :mixloop @14:38:53.90 PTV.dial :PRVmixing @14:38:53.98 PRV.dial CBV @14:38:54.00
CBV.dial PRV @14:38:54.23 CTV.dial :air,avoiding: :intake @14:38:54.25 Loading lyfil puck from tube 2 into Collection Clamp @14:38:54.48 SC.reconfigure :tight @14:38:54.53 SC.seek 2 @14:38:58.10 SC.configure :limp @14:38:58.72 Elbow.reconfigure :tight @14:38:58.80 Elbow.seek 2 @14:39:00.69 Forearm.reconfigure :tight @14:39:00.74 Forearm.seek 2 + 450 counts @14:39:01.83 SE.reconfigure :pick @14:39:01.89 SE.seek :beamBreak @14:39:04.87 SE.configure :default @14:39:05.03 Elbow.seek 2 @14:39:05.23 Forearm.seek 2 + 300 counts @14:39:05.39 Forearm.reconfigure :loose @14:39:05.44 Hand.close @14:39:06.12 SE.seek :down @14:39:06.32
Forearm.seek :retract @14:39:07.35 Elbow.seek CC @14:39:08.79 SE.stop @14:39:09.13
Elbow.reconfigure :loose @14:39:09.24 Forearm.reconfigure :tight @14:39:09.28 Forearm.seek CC @14:39:10.57 CC.seek :closed @14:39:15.10 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.11 @14:39:15.11 Hand.open @14:39:15.80 Forearm.reconfigure :loose @14:39:15.84 Forearm.seek :retract @14:39:17.25 CTV.dial CSV,avoiding: :intake @14:39:18.56 CSV.dial CTV @14:39:18.89 PS.seek 2.5ml @14:39:45.29 CS.seek 4.501ml @14:40:00.18 delay 8 seconds @14:40:08.20 PSV.dial :air @14:40:08.68 delay 2 seconds @14:40:10.69 PSV.dial :waste2 @14:40:11.43 PS.seek 0ml @14:40:37.81 CBV.dial between PRV and waste1 @14:40:38.15 CSV.dial :air @14:40:38.61 CS.seek 10ml @14:40:53.52 CSV.dial CTV @14:40:53.98 CS.seek 0ml @14:41:20.40 CBV.dial :waste1 @14:41:20.69 delay 5 seconds @14:41:25.72 CTV.dial :air,avoiding: :intake @14:41:27.10 Storing lyfil puck in Collection Clamp to tube 1 @14:41:27.27 SC.reconfigure :tight @14:41:27.32 SC.seek 1 @14:41:31.08 SC.configure :limp @14:41:31.30 Elbow.reconfigure :tight @14:41:31.34 Elbow.seek 1 @14:41:33.05 Forearm.reconfigure :tight @14:41:33.10 Forearm.seek 1 + 450 counts @14:41:34.14 SE.reconfigure :pick @14:41:34.20 SE.seek :beamBreak @14:41:37.26 SE.configure :default @14:41:37.42 Elbow.reconfigure :limp @14:41:37.47 Forearm.reconfigure :loose @14:41:37.52 Forearm.seek 1 @14:41:37.76 Elbow.hold @14:41:37.83 Elbow.reconfigure :tight @14:41:38.37 SE.seek 0.17 Pucks below Top Plate @14:41:38.79 Forearm.force -20 @14:41:38.84 Forearm.seek 1 + 309 counts @14:41:39.25 Forearm.reconfigure :tight @14:41:39.30 Forearm.seek 1 + 450 counts @14:41:39.50 SE.seek 1.17 Pucks below Top Plate @14:41:40.01 Forearm.reconfigure :loose @14:41:40.05 Forearm.seek :retract @14:41:40.97 Elbow.seek CC @14:41:42.63 Elbow.reconfigure :loose @14:41:42.73 Forearm.seek CC @14:41:43.70 Hand.close @14:41:44.36 CC.seek :open @14:41:49.13 Forearm.seek :retract @14:41:50.09 Elbow.reconfigure :tight @14:41:50.14 Elbow.seek 1 @14:41:51.88 Elbow.reconfigure :limp @14:41:51.93 Forearm.seek 1 @14:41:52.93 Elbow.hold @14:41:53.00 Elbow.reconfigure :tight @14:41:53.05 Hand.open @14:41:53.73 Forearm.force -20 @14:41:53.77 Forearm.seek 1 + 304 counts @14:41:54.16 Forearm.reconfigure :tight @14:41:54.22 Forearm.seek 1 + 450 counts @14:41:54.96 SE.seek :down @14:41:56.10
Forearm.reconfigure :loose @14:41:56.15 Forearm.seek :retract @14:41:57.58 SE.stop @14:41:57.62
PSV.dial :air @14:41:58.38 PS.seek 2.5ml @14:42:24.75 delay 1 second @14:42:25.77 PSV.dial :mixloop @14:42:26.22 delay 1 second @14:42:27.24 PSV.dial :air @14:42:27.71 delay 1 second @14:42:28.72 PSV.dial :mixloop @14:42:29.19 delay 1 second @14:42:30.20 PSV.dial :air @14:42:30.78 delay 1 second @14:42:31.80 PS.seek 0ml @14:42:58.19 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @14:42:58.37 Elbow.seek FlushPuck::Garage @14:43:00.19 Elbow.reconfigure :loose @14:43:00.30 Forearm.seek :garage @14:43:01.29 Hand.close @14:43:01.98 Forearm.seek :retract @14:43:02.95 Elbow.reconfigure :tight @14:43:03.00 Elbow.seek PC @14:43:04.26 Elbow.reconfigure :loose @14:43:04.36 Forearm.reconfigure :tight @14:43:04.40 Forearm.seek PC @14:43:05.60 PC.seek :closed @14:43:10.68 Processing Clamp onSpring/offSpring for 15/25 samples, on/off torque 1.60 @14:43:10.75 Hand.open @14:43:11.42 Forearm.reconfigure :loose @14:43:11.47 Forearm.seek :retract @14:43:12.46 PRV.dial :air @14:43:12.94 PSV.dial :mixloop @14:43:13.45 PS.seek 0.3ml @14:43:17.37 PSV.dial PSR @14:43:18.42 Pulling 0.4ml of PSR.air @14:43:18.43 PSR.select :air @14:43:18.45 PS.seek 0.7ml @14:43:23.37 delay 3 seconds @14:43:26.39 PSR.select :air @14:43:26.41 PS.seek 2.5ml @14:43:45.59 delay 3 seconds @14:43:48.61 PSV.dial :mixloop @14:43:49.65 PTV.dial :mixing @14:43:50.40 PBV.dial :waste2 @14:43:51.36 PS.seek 0ml @14:44:17.75 Garaging FlushPuck in Processing Clamp @14:44:17.82 Elbow.reconfigure :tight @14:44:17.87 Elbow.seek PC @14:44:17.99 Elbow.reconfigure :loose @14:44:18.09 Forearm.seek PC @14:44:19.04 Hand.close @14:44:19.61 PC.seek :open @14:44:24.33 Forearm.seek :retract @14:44:25.34 Elbow.reconfigure :tight @14:44:25.39 Elbow.seek :garage @14:44:26.75 Elbow.reconfigure :loose @14:44:26.85 Forearm.reconfigure :tight @14:44:26.90 Forearm.seek :garage @14:44:27.96 Hand.open @14:44:28.66 Forearm.reconfigure :loose @14:44:28.71 Forearm.seek :retract @14:44:29.69 Loading da puck from tube 2 into Processing Clamp @14:44:30.35 SC.reconfigure :tight @14:44:30.41 SC.seek 2 @14:44:33.99 SC.configure :limp @14:44:34.21 Elbow.reconfigure :tight @14:44:34.26 Elbow.seek 2 @14:44:36.10 Forearm.reconfigure :tight @14:44:36.15 Forearm.seek 2 + 450 counts @14:44:37.19 SE.reconfigure :pick @14:44:37.25 SE.seek :beamBreak @14:44:40.46 SE.configure :default @14:44:40.63 Elbow.seek 2 @14:44:40.81 Forearm.seek 2 + 300 counts @14:44:41.02 Forearm.reconfigure :loose @14:44:41.06 Hand.close @14:44:41.74 SE.seek :down @14:44:41.94
Forearm.seek :retract @14:44:42.92 Elbow.seek PC @14:44:44.44 SE.stop @14:44:45.45
Elbow.reconfigure :loose @14:44:45.56 Forearm.reconfigure :tight @14:44:45.60 Forearm.seek PC @14:44:46.87 PC.seek :closed @14:44:51.53 Processing Clamp onSpring/offSpring for 16/26 samples, on/off torque 1.60 @14:44:51.54 Hand.open @14:44:52.55 Forearm.reconfigure :loose @14:44:52.67 Forearm.seek :retract @14:44:53.64 Heating Puck.from 2, :da in Processing Clamp to 28C for 2:00:00 @14:44:53.66 PH.seek 28C,5:00 @14:45:21.19 PH.hold 2:00:00,28C @14:45:21.23 delay 1:30 @14:46:51.28 PSR.select :air @14:46:51.30 PSV.dial PSR @14:46:52.34 Pulling 0.5ml of PSR.wash @14:46:52.35 PSR.select :wash @14:46:52.47 PS.seek 0.5ml @14:46:58.40 delay 5 seconds @14:47:03.42 PSR.select :air @14:47:03.54 PS.seek 2ml @14:47:19.71 PTV.dial :puck @14:47:20.45 PSV.dial PTV @14:47:21.20 PBV.dial :waste1 @14:47:22.57 PS.seek 1.5ml @14:47:28.56 delay 3 seconds @14:47:31.57 PBV.dial between PSV and waste1 @14:47:31.94 PS.seek 0ml @14:47:48.15 delay 5:00 @14:52:48.17 recoverPSRreagent08 @14:52:48.18 PTV.dial :puck @14:52:48.25 PBV.dial :waste1 @14:52:48.63 delay 30 seconds @14:53:18.65 PBV.dial between PSV and waste1 @14:53:19.02 PSR.select :air @14:53:19.03 PSV.dial PSR @14:53:20.22 PS.reconfigure :fast @14:53:20.27 PS.seek 2.5ml @14:53:43.70 PSV.dial PTV @14:53:44.46 delay 5 seconds @14:53:49.48 PSV.dial PSR @14:53:50.35 delay 5 seconds @14:53:55.37 PSV.dial PTV @14:53:56.11 delay 5 seconds @14:54:01.13 PSV.dial :air @14:54:01.88 PS.seek 0ml @14:54:24.71 PTV.dial :PRVmixing,via: PRV @14:54:26.00 PSV.dial :mixloop @14:54:26.47 PRV.dial :ab1 @14:54:27.21 PS.reconfigure :slow @14:54:27.26 PS.seek 0.15ml @14:54:31.02 delay 2 seconds @14:54:33.04 CBV.dial PRV @14:54:33.06
PRV.dial CBV @14:54:33.65 PS.seek 0.34ml @14:54:38.05 delay 2 seconds @14:54:40.06 CBV.dial between PRV and PSV @14:54:40.38 PRV.dial :air @14:54:40.84 PS.seek 0.9ml @14:54:52.73 PRV.dial :waste2 @14:54:53.48 PS.seek 0.5ml @14:55:02.17 addPMLReagent08 @14:55:02.18 PSV.dial :air @14:55:02.76 PS.reconfigure :default @14:55:02.81 PS.seek 2ml @14:55:18.95 PSV.dial :PML @14:55:19.41 PTV.dial :mixing @14:55:20.14 PBV.dial :waste1 @14:55:20.56 PS.seek 1.85ml @14:55:22.98 PBV.dial between PSV and waste1 @14:55:23.35 PS.seek 0ml @14:55:43.26 delay 2 seconds @14:55:45.28 Beginning samplePassC @14:55:45.30 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:55:45.48 Elbow.reconfigure :tight @14:55:45.53 Elbow.seek FlushPuck::Garage @14:55:46.92 Elbow.reconfigure :loose @14:55:47.02 Forearm.seek :garage @14:55:47.97 Hand.close @14:55:49.15 Forearm.seek :retract @14:55:50.14 Elbow.reconfigure :tight @14:55:50.19 Elbow.seek CC @14:55:53.07 Elbow.reconfigure :loose @14:55:53.18 Forearm.reconfigure :tight @14:55:53.23 Forearm.seek CC @14:55:54.44 CC.seek :closed @14:55:58.97 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.39 @14:55:59.04 Hand.open @14:55:59.66 Forearm.reconfigure :loose @14:55:59.71 Forearm.seek :retract @14:56:00.67 PSV.dial PSR @14:56:01.72 Pulling 1.5ml of PSR.flush @14:56:01.73 PSR.select :flush @14:56:01.85 PS.seek 1.5ml @14:56:17.98 delay 5 seconds @14:56:23.00 PSV.dial :waste1 @14:56:23.47 PS.seek 0ml @14:56:39.64 PSV.dial PSR @14:56:40.11 PSR.select :air @14:56:40.23 PS.seek 2.5ml @14:57:06.55 PRV.dial CBV,avoiding: :ab2 @14:57:06.58
CBV.dial PRV @14:57:08.17 PSV.dial :mixloop @14:57:09.31 CTV.dial :air,avoiding: :intake @14:57:09.49 PTV.dial :PRVmixing @14:57:10.24 PS.seek 0ml @14:57:36.64 PSV.dial :air @14:57:37.11 PS.seek 2.5ml @14:58:03.42 PSV.dial :mixloop @14:58:04.33 PS.seek 0ml @14:58:30.73 PSV.dial :air @14:58:31.31 PS.seek 2.5ml @14:58:57.63 PSV.dial :mixloop @14:58:58.09 PS.seek 0ml @14:59:24.48 Garaging FlushPuck in Collection Clamp @14:59:24.55 Elbow.reconfigure :tight @14:59:24.60 Elbow.seek CC @14:59:24.84 Elbow.reconfigure :loose @14:59:24.94 Forearm.seek CC @14:59:25.97 Hand.close @14:59:26.60 CC.seek :open @14:59:31.21 Forearm.seek :retract @14:59:32.20 Elbow.reconfigure :tight @14:59:32.25 Elbow.seek :garage @14:59:35.16 Elbow.reconfigure :loose @14:59:35.26 Forearm.reconfigure :tight @14:59:35.31 Forearm.seek :garage @14:59:36.35 Hand.open @14:59:37.03 Forearm.reconfigure :loose @14:59:37.08 Forearm.seek :retract @14:59:38.05 delayUntil "15:03:45.27" @14:59:38.07 Whole Cell Archive sampling 50ml @14:59:38.09 Loading wcr puck from tube 2 into Collection Clamp @14:59:38.40 SC.reconfigure :tight @14:59:38.45 SC.seek 2 @14:59:38.59 SC.configure :limp @14:59:38.81 Elbow.reconfigure :tight @14:59:38.86 Elbow.seek 2 @14:59:40.76 Forearm.reconfigure :tight @14:59:41.25 Forearm.seek 2 + 450 counts @14:59:42.35 SE.reconfigure :pick @14:59:42.41 SE.seek :beamBreak @14:59:45.48 SE.configure :default @14:59:45.64 Elbow.seek 2 @14:59:45.84 Forearm.seek 2 + 300 counts @14:59:46.11 Forearm.reconfigure :loose @14:59:46.16 Hand.close @14:59:46.73 Puck::Warning in WCR -- Loaded Last remaining Puck (from Tube 2) @14:59:46.75 SE.seek :down @14:59:46.97 Forearm.seek :retract @14:59:47.95 Elbow.seek CC @14:59:49.53 SE.stop @14:59:49.64 Elbow.reconfigure :loose @14:59:49.75 Forearm.reconfigure :tight @14:59:49.80 Forearm.seek CC @14:59:51.18 CC.seek :closed @14:59:55.70 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.18 @14:59:55.71 Hand.open @14:59:56.40 Forearm.reconfigure :loose @14:59:56.46 Forearm.seek :retract @14:59:57.43 collectSample 50ml w/bubble point of 35psi @14:59:57.45 Priming 7ml @14:59:57.46 Intake.select :close @14:59:57.90 Exhaust.select :close @14:59:57.93 SS.hold @14:59:58.16 Calibrating pressure sensors @14:59:58.17 CTV.dial :air @14:59:58.19 SSV.dial :spit,avoiding: :exhaust @14:59:58.26 SS.reconfigure :fast @14:59:58.32 SS.seek 5ml @15:00:01.17 SSV.dial CBV @15:00:01.20 CBV.dial SSV @15:00:02.54 delay 3.8 seconds @15:00:06.47 Sensor limits: Intake max 118.3psia; Outlet max 111.3psia @15:00:06.48 SSV.dial :spit @15:00:07.82 SS.seek 0ml @15:00:10.46 SSV.dial CBV @15:00:11.77 CTV.dial :intake @15:00:12.52 SSV.dial CBV @15:00:12.55 CBV.dial SSV @15:00:12.69 Allowing 40s for 7.0ml of remaining 7.0ml @15:00:12.71 Intake.select :open @15:00:14.88 Intake @15.5psia, vacuum from 10.0 to 13.0psi @15:00:14.90 SS.reconfigure :inhaling 2.20ml/s @15:00:14.95 SS.seek 7ml @15:00:21.47 Stroke completed -- waiting for pressure to equalize ... @15:00:21.49 SS.reconfigure :exhale @15:00:21.53 SS.hold @15:00:27.62 Intake.select :close @15:00:27.78 SSV.dial :exhaust @15:00:28.54 Exhaust.select :open @15:00:28.65 SS.seek 0ml @15:00:32.68 Exhaust.select :close @15:00:32.79 Sampling 50.0ml @15:00:32.80 Intake.select :close @15:00:32.81 Exhaust.select :close @15:00:32.82 SS.reconfigure :default @15:00:32.87 SS.hold @15:00:33.08 CTV.dial :intake @15:00:33.16 SSV.dial CBV @15:00:33.19 CBV.dial SSV @15:00:33.99 Allowing 130s for 25.0ml of remaining 50.0ml @15:00:34.01 Intake.select :open @15:00:36.18 Intake @15.9psia, vacuum from 10.0 to 13.0psi @15:00:36.20 SS.reconfigure :inhaling 2.20ml/s @15:00:36.25 SS.seek 25ml @15:00:50.75 Vacuum too high -- waiting up to 40 seconds @15:00:50.77 SS.reconfigure :exhale @15:00:50.82 SS.hold @15:00:51.40 Intake @12.7psia, vacuum from 7.2 to 10.2psi @15:00:51.42 SS.reconfigure :inhaling 1.76ml/s @15:00:51.47 SS.seek 25ml @15:00:52.07 Stroke completed -- waiting for pressure to equalize ... @15:00:52.08 SS.reconfigure :exhale @15:00:52.13 SS.hold @15:00:57.50 Intake.select :close @15:00:57.66 SSV.dial :exhaust @15:00:58.41 Exhaust.select :open @15:00:58.53 SS.seek 0ml @15:01:07.40 Exhaust.select :close @15:01:07.51 SSV.dial CBV @15:01:07.54 CBV.dial SSV @15:01:08.33 Allowing 130s for 25.0ml of remaining 25.0ml @15:01:08.35 Intake.select :open @15:01:10.52 Intake @16.0psia, vacuum from 10.0 to 13.0psi @15:01:10.54 SS.reconfigure :inhaling 1.76ml/s @15:01:10.59 SS.seek 25ml @15:01:27.43 Vacuum too high -- waiting up to 40 seconds @15:01:27.45 SS.reconfigure :exhale @15:01:27.51 SS.hold @15:01:29.79 Intake @13.8psia, vacuum from 8.3 to 11.3psi @15:01:29.81 SS.reconfigure :inhaling 1.41ml/s @15:01:29.86 SS.seek 25ml @15:01:31.27 Stroke completed -- waiting for pressure to equalize ... @15:01:31.28 SS.reconfigure :exhale @15:01:31.33 SS.hold @15:01:35.33 Intake.select :close @15:01:35.49 SSV.dial :exhaust @15:01:36.24 Exhaust.select :open @15:01:36.36 SS.seek 0ml @15:01:45.27 Exhaust.select :close @15:01:45.38 Sampled 50.0ml @15:01:45.40 Drying puck before fixing for 5:00 @15:01:45.41 CBV.dial :waste2b @15:01:46.16 delay 1:00 @15:02:46.19 CSV.dial :air @15:02:46.65 CS.reconfigure :fast @15:02:46.70 CS.seek 10ml @15:03:11.31 Pumping wcr puck from tube 2 up to 35psi... @15:03:11.33 CBV.dial SSV @15:03:12.58 CBV.dial between CSV and waste2b @15:03:13.54 CSV.dial between air and CTV @15:03:13.87 CS.seek 7.292ml @15:03:20.88 CTV.dial CSV,avoiding: :air @15:03:20.91 CSV.dial CTV @15:03:21.69 CS.reconfigure :pressurize @15:03:21.75 CS.seek 0ml @15:03:37.15 CBV.dial :waste2b @15:03:37.49 CS.reconfigure :default @15:03:37.54 CS.seek 0ml @15:03:42.00 delay 1:00 @15:03:45.28 recoverPRVreagent08 @15:03:45.30 PTV.dial :mixing @15:03:46.04 PBV.dial :waste1 @15:03:46.40 delay 30 seconds @15:04:16.42 PBV.dial between PSV and waste1 @15:04:16.79 PSV.dial :air @15:04:17.26 PS.reconfigure :fast @15:04:17.31 PS.seek 2.5ml @15:04:41.03 PSV.dial :mixloop @15:04:41.50 delay 2 seconds @15:04:42.02 CTV.dial :air,avoiding: :intake @15:04:43.33 Fix #1 of 2 (with RNAlater) for 2:30 @15:04:43.35 CSV.dial CSR @15:04:43.52 PSV.dial :air @15:04:43.78 Pulling 2ml of CSR.RNAlater @15:04:43.79 CSR.select :RNAlater @15:04:43.91 CS.seek 2ml @15:04:43.99 delay 2 seconds @15:04:46.01 PSV.dial :mixloop @15:04:46.47 delay 2 seconds @15:04:48.49 PSV.dial :air @15:04:49.40 PS.seek 0ml @15:04:49.84 delay 5 seconds @15:04:54.86 CSR.select :air @15:04:54.98 CS.reconfigure :fast @15:04:55.03 CS.seek 10ml @15:05:12.76 washCycleDA #1 of 2 @15:05:12.77 PSR.select :air @15:05:12.78 PSV.dial PSR @15:05:14.10 Pulling 0.5ml of PSR.wash @15:05:14.11 PSR.select :wash @15:05:14.23 PS.reconfigure :default @15:05:14.28 PS.seek 0.5ml @15:05:14.75 Pumping wcr puck from tube 2 up to 35psi... @15:05:14.77 CBV.dial SSV @15:05:16.00 CBV.dial between CSV and waste2b @15:05:16.96 CSV.dial between air and CTV @15:05:17.57 CS.seek 7.275ml @15:05:20.22 delay 5 seconds @15:05:24.69 CTV.dial CSV,avoiding: :air @15:05:24.72 CSV.dial CTV @15:05:25.24 PSR.select :air @15:05:25.36 PS.seek 2ml @15:05:26.08 CS.reconfigure :pressurize @15:05:26.14 CS.seek 0ml @15:05:41.39 delay 2:30 @15:05:41.53 PTV.dial :puck @15:05:42.27 PSV.dial PTV @15:05:43.13 PBV.dial :waste1 @15:05:43.55 PS.seek 1.5ml @15:05:49.53 delay 3 seconds @15:05:52.55 PBV.dial between PSV and waste1 @15:05:52.91 PS.seek 0ml @15:06:09.22 delay 2:00 @15:08:09.25 recoverPSRreagent08 @15:08:09.26 PTV.dial :puck @15:08:09.34 PBV.dial :waste1 @15:08:09.71 delay 30 seconds @15:08:11.41 CBV.dial :waste2b @15:08:11.75 CS.reconfigure :default @15:08:11.80 CS.seek 0ml @15:08:16.59 delay 1:00 @15:08:39.72 PBV.dial between PSV and waste1 @15:08:40.08 PSR.select :air @15:08:40.10 PSV.dial PSR @15:08:40.85 PS.reconfigure :fast @15:08:40.90 PS.seek 2.5ml @15:09:04.53 PSV.dial PTV @15:09:05.30 delay 5 seconds @15:09:10.32 PSV.dial PSR @15:09:11.09 delay 5 seconds @15:09:16.11 PSV.dial PTV @15:09:16.61 CTV.dial :air,avoiding: :intake @15:09:16.86 delay 5 seconds @15:09:17.94 Fix #2 of 2 (with RNAlater) for 2:30 @15:09:17.95 CSV.dial CSR @15:09:18.38 Pulling 2ml of CSR.RNAlater @15:09:18.40 CSR.select :RNAlater @15:09:18.51 CS.seek 2ml @15:09:21.88 PSV.dial :air @15:09:22.63 PS.seek 0ml @15:09:24.40 delay 5 seconds @15:09:29.42 CSR.select :air @15:09:29.53 CS.reconfigure :fast @15:09:29.59 CS.seek 10ml @15:09:45.84 washCycleDA #2 of 2 @15:09:45.85 PSR.select :air @15:09:45.87 PSV.dial PSR @15:09:47.19 Pulling 0.5ml of PSR.wash @15:09:47.20 PSR.select :wash @15:09:47.32 PS.reconfigure :default @15:09:47.37 PS.seek 0.5ml @15:09:49.56 Pumping wcr puck from tube 2 up to 35psi... @15:09:49.58 CBV.dial SSV @15:09:50.81 CBV.dial between CSV and waste2b @15:09:51.77 CSV.dial between air and CTV @15:09:52.38 CS.seek 7.283ml @15:09:53.40 delay 5 seconds @15:09:58.42 PSR.select :air @15:09:58.54 PS.seek 2ml @15:09:59.44 CTV.dial CSV,avoiding: :air @15:09:59.47 CSV.dial CTV @15:10:00.81 CS.reconfigure :pressurize @15:10:00.87 CS.seek 0ml @15:10:14.75 PTV.dial :puck @15:10:14.82 PSV.dial PTV @15:10:15.30 delay 2:30 @15:10:15.58 PBV.dial :waste1 @15:10:16.00 PS.seek 1.5ml @15:10:22.00 delay 3 seconds @15:10:25.02 PBV.dial between PSV and waste1 @15:10:25.40 PS.seek 0ml @15:10:41.59 delay 2:00 @15:12:41.62 recoverPSRreagent08 @15:12:41.63 PTV.dial :puck @15:12:41.70 PBV.dial :waste1 @15:12:42.07 delay 30 seconds @15:12:45.32 CBV.dial :waste2b @15:12:45.65 CS.reconfigure :default @15:12:46.15 CS.seek 0ml @15:12:51.52 delay 1:00 @15:13:12.09 PBV.dial between PSV and waste1 @15:13:12.47 PSR.select :air @15:13:12.48 PSV.dial PSR @15:13:13.36 PS.reconfigure :fast @15:13:13.41 PS.seek 2.5ml @15:13:37.09 PSV.dial PTV @15:13:37.82 delay 5 seconds @15:13:42.84 PSV.dial PSR @15:13:43.59 delay 5 seconds @15:13:48.61 PSV.dial PTV @15:13:49.35 delay 5 seconds @15:13:51.54 CTV.dial :air,avoiding: :intake @15:13:54.37 PSV.dial :air @15:13:55.14 PS.seek 0ml @15:14:18.31 getPMLReagent08 @15:14:18.32 PRV.dial :air @15:14:18.74 delay 5 seconds @15:14:23.77 PTV.dial :PRVmixing @15:14:25.06 delay 5 seconds @15:14:30.08 PRV.dial :ab2 @15:14:30.52 PTV.dial :PRVmixing @15:14:30.60 PSV.dial :mixloop @15:14:31.07 PS.reconfigure :default @15:14:31.12 PS.seek 0.3ml @15:14:34.96 delay 2 seconds @15:14:36.98 PRV.dial :air @15:14:37.49 PS.seek 0.55ml @15:14:40.94 addPMLReagent08 @15:14:40.95 PSV.dial :air @15:14:41.42 PS.seek 2ml @15:14:57.11 PSV.dial :PML @15:14:57.58 PTV.dial :mixing @15:14:58.33 PBV.dial :waste1 @15:14:58.77 PS.seek 1.85ml @15:15:01.18 PBV.dial between PSV and waste1 @15:15:01.56 PS.seek 0ml @15:15:21.27 delay 2 seconds @15:15:23.29 delay 8:00 @15:23:23.32 recoverPRVreagent08 @15:23:23.33 PTV.dial :mixing @15:23:23.40 PBV.dial :waste1 @15:23:23.78 delay 30 seconds @15:23:53.81 PBV.dial between PSV and waste1 @15:23:54.18 PSV.dial :air @15:23:54.66 PS.reconfigure :fast @15:23:54.71 PS.seek 2.5ml @15:24:18.54 PSV.dial :mixloop @15:24:19.01 delay 2 seconds @15:24:21.03 PSV.dial :air @15:24:21.50 delay 2 seconds @15:24:23.51 PSV.dial :mixloop @15:24:23.98 delay 2 seconds @15:24:26.00 PSV.dial :air @15:24:26.47 PS.seek 0ml @15:24:49.83 washCycleDA #1 of 3 @15:24:49.84 PSR.select :air @15:24:49.85 PSV.dial PSR @15:24:51.62 Pulling 0.5ml of PSR.wash @15:24:51.63 PSR.select :wash @15:24:51.75 PS.reconfigure :default @15:24:51.81 PS.seek 0.5ml @15:24:57.82 delay 5 seconds @15:25:02.84 PSR.select :air @15:25:02.97 PS.seek 2ml @15:25:19.20 PTV.dial :puck @15:25:19.93 PSV.dial PTV @15:25:20.68 PBV.dial :waste1 @15:25:21.12 PS.seek 1.5ml @15:25:27.35 delay 3 seconds @15:25:30.37 PBV.dial between PSV and waste1 @15:25:30.75 PS.seek 0ml @15:25:46.89 delay 2:00 @15:27:46.92 recoverPSRreagent08 PTV.dial :puck @15:27:46.99 PBV.dial :waste1 @15:27:47.37 delay 30 seconds @15:28:17.39 PBV.dial between PSV and waste1 @15:28:17.76 PSR.select :air @15:28:17.78 PSV.dial PSR @15:28:18.54 PS.reconfigure :fast @15:28:18.59 PS.seek 2.5ml @15:28:42.25 PSV.dial PTV @15:28:43.01 delay 5 seconds @15:28:48.03 PSV.dial PSR @15:28:48.77 delay 5 seconds @15:28:53.79 PSV.dial PTV @15:28:54.54 delay 5 seconds @15:28:59.56 PSV.dial :air @15:29:00.31 PS.seek 0ml @15:29:23.70 washCycleDA #2 of 3 @15:29:23.71 PSR.select :air @15:29:23.72 PSV.dial PSR @15:29:25.04 Pulling 0.5ml of PSR.wash @15:29:25.05 PSR.select :wash @15:29:25.17 PS.reconfigure :default @15:29:25.22 PS.seek 0.5ml @15:29:31.19 delay 5 seconds @15:29:36.21 PSR.select :air @15:29:36.34 PS.seek 2ml @15:29:52.47 PTV.dial :puck @15:29:52.55 PSV.dial PTV @15:29:53.41 PBV.dial :waste1 @15:29:53.85 PS.seek 1.5ml @15:29:59.72 delay 3 seconds @15:30:02.74 PBV.dial between PSV and waste1 @15:30:03.12 PS.seek 0ml @15:30:19.29 delay 2:00 @15:32:19.32 recoverPSRreagent08 PTV.dial :puck @15:32:19.39 PBV.dial :waste1 @15:32:19.77 delay 30 seconds @15:32:49.79 PBV.dial between PSV and waste1 @15:32:50.16 PSR.select :air @15:32:50.18 PSV.dial PSR @15:32:50.91 PS.reconfigure :fast @15:32:50.96 PS.seek 2.5ml @15:33:14.69 PSV.dial PTV @15:33:15.56 delay 5 seconds @15:33:20.99 PSV.dial PSR @15:33:21.78 delay 5 seconds @15:33:26.80 PSV.dial PTV @15:33:27.67 delay 5 seconds @15:33:32.69 PSV.dial :air @15:33:33.46 PS.seek 0ml @15:33:56.81 washCycleDA #3 of 3 @15:33:56.82 PSR.select :air @15:33:56.84 PSV.dial PSR @15:33:58.16 Pulling 0.5ml of PSR.wash @15:33:58.17 PSR.select :wash @15:33:58.29 PS.reconfigure :default @15:33:58.34 PS.seek 0.5ml @15:34:04.22 delay 5 seconds @15:34:09.24 PSR.select :air @15:34:09.36 PS.seek 2ml @15:34:25.47 PTV.dial :puck @15:34:25.55 PSV.dial PTV @15:34:26.28 PBV.dial :waste1 @15:34:26.70 PS.seek 1.5ml @15:34:32.69 delay 3 seconds @15:34:35.71 PBV.dial between PSV and waste1 @15:34:36.07 PS.seek 0ml @15:34:52.38 delay 2:00 @15:36:52.40 recoverPSRreagent08 @15:36:52.41 PTV.dial :puck @15:36:52.48 PBV.dial :waste1 @15:36:52.84 delay 30 seconds @15:37:22.87 PBV.dial between PSV and waste1 @15:37:23.23 PSR.select :air @15:37:23.25 PSV.dial PSR @15:37:24.00 PS.reconfigure :fast @15:37:24.05 PS.seek 2.5ml @15:37:47.71 PSV.dial PTV @15:37:48.59 delay 5 seconds @15:37:53.61 PSV.dial PSR @15:37:54.34 delay 5 seconds @15:37:59.36 PSV.dial PTV @15:38:00.12 delay 5 seconds @15:38:05.14 PSV.dial :air @15:38:05.89 PS.seek 0ml @15:38:29.12 Gate.power :camera,:ON @15:38:29.13 Mixing DA Substrates @15:38:29.14 PSR.select :air @15:38:29.16 PSV.dial PSR @15:38:30.54 Pulling 0.25ml of PSR.sub1 @15:38:30.57 PSR.select :sub1 @15:38:30.82 PS.reconfigure :default @15:38:30.93 PS.seek 0.25ml @15:38:34.41 delay 5 seconds @15:38:39.43 Pulling 0.25ml of PSR.sub2 @15:38:39.45 PSR.select :sub2 @15:38:39.56 PS.seek 0.5ml @15:38:42.96 delay 5 seconds @15:38:47.98 PSR.select :air @15:38:48.10 PS.seek 2ml @15:39:04.27 PTV.dial :puck @15:39:04.35 PSV.dial PTV @15:39:05.10 PBV.dial :waste1 @15:39:05.54 PS.seek 1.5ml @15:39:11.46 delay 3 seconds @15:39:14.48 PBV.dial between PSV and waste1 @15:39:14.86 PS.seek 0ml @15:39:31.09 delay 10 seconds @15:39:41.18 recoverPSRreagent08 @15:39:41.19 PTV.dial :puck @15:39:41.26 PBV.dial :waste1 @15:39:41.63 delay 30 seconds @15:40:11.66 PBV.dial between PSV and waste1 @15:40:12.04 PSR.select :air @15:40:12.06 PSV.dial PSR @15:40:12.82 PS.reconfigure :fast @15:40:12.88 PS.seek 2.5ml @15:40:36.96 PSV.dial PTV @15:40:37.73 delay 5 seconds @15:40:42.75 PSV.dial PSR @15:40:43.51 delay 5 seconds @15:40:48.53 PSV.dial PTV @15:40:49.29 delay 5 seconds @15:40:54.31 PSV.dial :air @15:40:55.08 PS.seek 0ml @15:41:18.80 Additional air clear @15:41:18.82 PSR.select :air @15:41:18.83 PSV.dial PSR @15:41:20.18 Pulling 2ml of PSR.air @15:41:20.19 PSR.select :air @15:41:20.21 PS.reconfigure :default @15:41:20.26 PS.seek 2ml @15:41:41.58 delay 5 seconds @15:41:46.61 PTV.dial :puck @15:41:46.69 PSV.dial PTV @15:41:47.44 PBV.dial :waste1 @15:41:47.88 PS.seek 1.5ml @15:41:53.90 delay 3 seconds @15:41:56.92 PBV.dial between PSV and waste1 @15:41:57.29 delay 1 second @15:41:58.31 PSV.dial :waste1 @15:41:59.49 PS.seek 0ml @15:42:15.89 Processing Puck Heater at 28C before imaging @15:42:15.90 PH.coast @15:42:16.01 Imaging da puck in Processing Clamp while storing it to tube 1 @15:42:16.18 SC.reconfigure :tight @15:42:16.23 SC.seek 1 @15:42:20.00 SC.configure :limp @15:42:20.22 Elbow.reconfigure :tight @15:42:20.27 Elbow.seek 1 @15:42:22.08 Forearm.reconfigure :tight @15:42:22.13 Forearm.seek 1 + 450 counts @15:42:23.24 SE.reconfigure :pick @15:42:23.30 SE.seek :beamBreak @15:42:26.35 SE.configure :default @15:42:26.51 Elbow.reconfigure :limp @15:42:26.57 Forearm.reconfigure :loose @15:42:26.62 Forearm.seek 1 @15:42:27.35 Elbow.hold @15:42:27.42 Elbow.reconfigure :tight @15:42:27.53 SE.seek 1.17 Pucks below Top Plate @15:42:27.97 Forearm.force -20 @15:42:28.02 Forearm.seek 1 + 310 counts @15:42:28.33 Forearm.reconfigure :tight @15:42:28.39 Forearm.seek 1 + 450 counts @15:42:28.67 SE.seek 2.17 Pucks below Top Plate @15:42:29.18 Forearm.reconfigure :loose @15:42:29.24 Forearm.seek :retract @15:42:30.23 Elbow.seek PC @15:42:32.72 Elbow.reconfigure :loose @15:42:32.82 Forearm.seek PC @15:42:33.76 Hand.close @15:42:34.43 PC.seek :open @15:42:39.20 Forearm.seek :retract @15:42:40.14 Elbow.reconfigure :tight @15:42:40.19 Elbow.seek 1 @15:42:42.77 Forearm.reconfigure :tight @15:42:42.82 Forearm.seek 1 + 450 counts @15:42:43.79 Hand.open @15:42:44.51 SE.seek 1.82 Pucks below Top Plate @15:42:44.79 SE.reconfigure :seal @15:42:44.84 SE.seek 0.86 Pucks below Top Plate @15:42:45.35 Lights, camera, ACTION! @15:42:45.38 PuckCamera: snap -bin=2,2 -auto /var/log/esp/da24jan1815h50ml.tif @15:42:52.92 SX HX9/16 (USB:03): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 3.286 seconds /var/log/esp/da24jan1815h50ml.tif: TIFF Upload Complete @15:42:52.95 PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da24jan1815h50ml15s.tif @15:43:04.89 Waiting >20 seconds for da to relinquish FlushPuck @15:43:08.58 SX HX9/16 (USB:03): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15 seconds /var/log/esp/midres/da24jan1815h50ml15s.tif: TIFF Upload Complete @15:43:08.61 PuckCamera: snap -bin=2,2 20.0 /var/log/esp/midres/da24jan1815h50ml20s.tif @15:43:29.26 SX HX9/16 (USB:03): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 20 seconds /var/log/esp/midres/da24jan1815h50ml20s.tif: TIFF Upload Complete @15:43:29.28 Gate.power :camera,:OFF @15:43:29.29 DAfans.email "3.286 sec auto-exposure",Subject: "da24jan1815h50ml images!" @15:43:29.71 Elbow.seek 1 @15:43:29.96 Forearm.seek 1 + 450 counts @15:43:30.68 SE.reconfigure :gently @15:43:30.74 SE.seek 1.57 Pucks below Top Plate @15:43:31.38 Elbow.reconfigure :limp @15:43:31.45 Forearm.reconfigure :loose @15:43:31.49 Forearm.seek 1 @15:43:31.77 Elbow.hold @15:43:31.85 Elbow.reconfigure :tight @15:43:31.94 SE.reconfigure :default @15:43:31.99 SE.seek 1.76 Pucks below Top Plate @15:43:32.38 Forearm.force -20 @15:43:32.42 Forearm.seek 1 + 316 counts @15:43:33.17 Forearm.reconfigure :tight @15:43:33.25 Forearm.seek 1 + 450 counts @15:43:33.52 SE.seek :down @15:43:34.81 Forearm.reconfigure :loose @15:43:34.93 Forearm.seek :retract @15:43:36.14 SE.stop @15:43:36.37 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @15:43:36.95 Elbow.seek FlushPuck::Garage @15:43:38.87 Elbow.reconfigure :loose @15:43:39.12 Forearm.seek :garage @15:43:40.23 Hand.close @15:43:41.25 Forearm.seek :retract @15:43:42.32 Elbow.reconfigure :tight @15:43:42.42 Elbow.seek PC @15:43:43.79 Elbow.reconfigure :loose @15:43:44.04 Forearm.reconfigure :tight @15:43:44.15 Forearm.seek PC @15:43:46.96 PC.seek :closed @15:43:50.07 upsync @15:43:51.62 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.65 @15:43:51.69 Hand.open @15:43:52.37 Forearm.reconfigure :loose @15:43:52.41 Forearm.seek :retract @15:43:53.43 PSR.select :air @15:43:53.46 Storing wcr puck in Collection Clamp to tube 1 @15:43:53.48 PSV.dial PSR @15:43:53.72 SC.reconfigure :tight @15:43:53.77 SC.seek 1 @15:43:53.96 Pulling 0.5ml of PSR.flush @15:43:53.97 PSR.select :flush @15:43:54.06 SC.configure :limp @15:43:54.21 PS.seek 0.5ml @15:43:54.35 Elbow.reconfigure :tight @15:43:54.40 Elbow.seek 1 @15:43:57.02 Forearm.reconfigure :tight @15:43:57.07 Forearm.seek 1 + 450 counts @15:43:58.09 SE.reconfigure :pick @15:43:58.15 SE.seek :beamBreak @15:44:00.08 delay 5 seconds @15:44:01.10 SE.configure :default @15:44:01.26 Elbow.reconfigure :limp @15:44:01.32 Forearm.reconfigure :loose @15:44:01.37 Forearm.seek 1 @15:44:01.65 Elbow.hold @15:44:02.15 Elbow.reconfigure :tight @15:44:02.37 SE.seek 2.17 Pucks below Top Plate @15:44:02.80 Forearm.force -20 @15:44:02.85 Forearm.seek 1 + 318 counts @15:44:03.24 Forearm.reconfigure :tight @15:44:03.29 Forearm.seek 1 + 450 counts @15:44:03.48 SE.seek 3.17 Pucks below Top Plate @15:44:04.00 Forearm.reconfigure :loose @15:44:04.05 Forearm.seek :retract @15:44:05.04 Elbow.seek CC @15:44:05.11 PSR.select :air @15:44:05.23 PS.seek 2.5ml @15:44:06.69 Elbow.reconfigure :loose @15:44:06.80 Forearm.seek CC @15:44:07.77 Hand.close @15:44:08.43 CC.seek :open @15:44:13.16 Forearm.seek :retract @15:44:14.12 Elbow.reconfigure :tight @15:44:14.17 Elbow.seek 1 @15:44:15.98 Elbow.reconfigure :limp @15:44:16.04 Forearm.seek 1 @15:44:17.04 Elbow.hold @15:44:17.11 Elbow.reconfigure :tight @15:44:17.16 Hand.open @15:44:17.94 Forearm.force -20 @15:44:18.36 Forearm.seek 1 + 319 counts @15:44:18.76 Forearm.reconfigure :tight @15:44:18.82 Forearm.seek 1 + 450 counts @15:44:19.11 SE.seek :down @15:44:20.25 Forearm.reconfigure :loose @15:44:20.30 Forearm.seek :retract @15:44:21.64 SE.stop @15:44:21.68
Starting finalPurge @15:44:26.49 delay 1 second @15:44:27.51 PRV.dial :waste2,avoiding: :ab2 @15:44:29.35 PTV.dial :PRVmixing @15:44:30.64 PSV.dial :mixloop @15:44:31.67 PS.seek :empty @15:44:41.37
Waiting >20 seconds for psFlush to relinquish FlushPuck @15:44:58.08 PSR.select :air @15:44:58.10 PSV.dial PSR @15:44:59.13 Pulling 0.5ml of PSR.flush @15:44:59.14 PSR.select :flush @15:44:59.26 PS.seek 0.5ml @15:45:05.27 delay 5 seconds @15:45:10.29 PSR.select :air @15:45:10.41 PS.seek 2.5ml @15:45:31.61 delay 1 second @15:45:32.63 PBV.dial :waste1 @15:45:33.00 PTV.dial :mixing @15:45:33.75 PSV.dial :mixloop @15:45:34.91 PS.seek :empty @15:46:01.27 PSV.dial PSR @15:46:02.41 PSR.select :flush @15:46:02.53 PS.seek 1ml @15:46:13.54 PSR.select :air @15:46:13.67 PS.seek 2.5ml @15:46:29.80 PSV.dial PTV @15:46:30.53 PTV.dial :puck @15:46:31.27 PBV.dial :waste1 @15:46:31.46 PS.seek 0ml @15:46:57.83 PSV.dial PSR @15:46:58.58 PSR.select :flush @15:46:58.70 PS.seek 0.5ml @15:47:04.67 PSR.select :air @15:47:04.79 PS.seek 2.5ml @15:47:26.01 PSV.dial PTV @15:47:26.76 PBV.dial PSV @15:47:27.75 PS.seek 0ml @15:47:54.14 PSV.dial PBV @15:47:54.61 PS.seek 2.5ml @15:48:20.95 PSV.dial :waste1 @15:48:21.69 PS.seek 0ml @15:48:48.11 PSV.dial PSR @15:48:48.57 PSR.select :air @15:48:48.60 PS.seek 2.5ml @15:49:14.98 PSV.dial PTV @15:49:15.82 PBV.dial PSV @15:49:16.02 PS.seek 0ml @15:49:42.54 PSV.dial PBV @15:49:43.00 PS.seek 2.5ml @15:50:09.38 PSV.dial :waste1 @15:50:10.13 PS.seek 0ml @15:50:36.45 PSV.dial PSR @15:50:36.90 PSR.select :flush @15:50:37.03 PS.seek 1ml @15:50:48.10 PSR.select :air @15:50:48.22 PS.seek 2.5ml @15:51:04.45 PSV.dial PTV @15:51:05.18 PTV.dial :puck @15:51:05.26 PBV.dial :waste1 @15:51:05.81 PS.seek 0ml @15:51:32.35 PSV.dial PSR @15:51:33.10 PSR.select :flush @15:51:33.23 PS.seek 0.5ml @15:51:39.13 PSR.select :air @15:51:39.25 PS.seek 2.5ml @15:52:00.69 PSV.dial PTV @15:52:01.45 PBV.dial PSV @15:52:02.01 PS.seek 0ml @15:52:28.32 PSV.dial PBV @15:52:28.80 PS.seek 2.5ml @15:52:55.22 PSV.dial :waste1 @15:52:55.97 PS.seek 0ml @15:53:22.35 PSV.dial PSR @15:53:22.82 PSR.select :air @15:53:22.84 PS.seek 2.5ml @15:53:49.25 PSV.dial PTV @15:53:49.99 PBV.dial PSV @15:53:50.18 PS.seek 0ml @15:54:17.10 PSV.dial PBV @15:54:17.64 PS.seek 2.5ml @15:54:44.00 PSV.dial :waste1 @15:54:44.75 PS.seek 0ml @15:55:11.32
Loading FlushPuck into Collection Clamp from Processing Clamp @15:55:11.51 Elbow.seek PC @15:55:13.99 Elbow.reconfigure :loose @15:55:14.09 Forearm.seek PC @15:55:15.04 Hand.close @15:55:15.67 PC.seek :open @15:55:20.41 Forearm.seek :retract @15:55:21.41 Elbow.reconfigure :tight @15:55:21.46 Elbow.seek CC @15:55:25.04 Elbow.reconfigure :loose @15:55:25.14 Forearm.reconfigure :tight @15:55:25.19 Forearm.seek CC @15:55:26.46 CC.seek :closed @15:55:30.98 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.42 @15:55:31.05 Hand.open @15:55:31.73 Forearm.reconfigure :loose @15:55:31.78 Forearm.seek :retract @15:55:32.75 Flushing the Collection Valve::Manifold @15:55:32.89 CSV.dial CSR @15:55:33.34 Pulling 5ml of CSR.flush CSR.select :flush @15:55:33.46 CS.seek 5ml @15:55:47.29 delay 5 seconds @15:55:52.31 CSR.select :air @15:55:52.48 CS.reconfigure :fast @15:55:52.97 CS.seek 7.499ml @15:55:59.72 CSV.dial :waste @15:56:01.01 CS.reconfigure :default @15:56:01.06 CS.seek 0ml @15:56:21.10 Flushing lower manifold's waste1 port @15:56:21.23 CSV.dial :flush @15:56:21.79 CS.reconfigure :fast @15:56:21.84 CS.seek 5ml @15:56:35.04 delay 5 seconds @15:56:40.06 CSV.dial :air @15:56:40.59 CS.seek 7.001ml @15:56:46.32 CSV.dial CTV @15:56:46.35
CTV.dial CSV @15:56:47.71 CBV.dial :waste1 @15:56:48.47 CS.seek 0ml @15:57:05.13 Flushing lower manifold's waste2 port @15:57:05.26 CSV.dial :flush @15:57:06.00 CS.seek 5ml @15:57:19.10 delay 5 seconds @15:57:24.12 CSV.dial :air @15:57:24.65 CS.seek 7.002ml @15:57:30.20 CSV.dial CTV @15:57:30.23
CTV.dial CSV @15:57:30.71 CBV.dial :waste2 @15:57:31.73 CS.seek 0ml @15:57:48.35 Flushing lower manifold's waste2b port @15:57:48.47 CSV.dial :flush @15:57:49.20 CS.seek 5ml @15:58:02.35 delay 5 seconds @15:58:07.37 CSV.dial :air @15:58:07.88 CS.seek 7ml @15:58:13.44 CSV.dial CTV @15:58:13.47
CTV.dial CSV @15:58:13.91 CBV.dial :waste2b @15:58:14.95 CS.seek 0ml @15:58:31.57 Disinfecting external Intake with kill @15:58:31.69 CSV.dial CSR @15:58:32.13 Pulling 8ml of CSR.kill @15:58:32.14 CSR.select :kill @15:58:32.26 CS.seek 8ml @15:58:52.07 delay 5 seconds @15:58:57.09 CSR.select :air @15:58:57.21 CS.seek 10ml @15:59:02.79 CSV.dial CBV @15:59:02.81
CBV.dial CSV @15:59:04.30 CTV.dial :intake @15:59:05.04 Intake.select :open @15:59:05.15 CS.seek 2ml @15:59:24.00 Intake.select :close @15:59:24.11 CTV.dial :air @15:59:24.87 CS.reconfigure :default @15:59:24.92 CS.seek 0ml @15:59:30.88 CSV.dial CSR @15:59:31.89 Pulling 5ml of CSR.flush @15:59:31.90 CSR.select :flush @15:59:32.03 CS.seek 5ml @15:59:45.66 delay 5 seconds @15:59:50.68 CSR.select :air @15:59:50.80 CS.seek 10ml @16:00:04.38 CSV.dial CBV @16:00:05.38 CS.seek 0ml @16:00:31.82 Garaging FlushPuck in Collection Clamp @16:00:31.89 Elbow.reconfigure :tight @16:00:31.94 Elbow.seek CC @16:00:32.14 Elbow.reconfigure :loose @16:00:32.24 Forearm.seek CC @16:00:33.24 Hand.close @16:00:33.79 CC.seek :open @16:00:38.46 Forearm.seek :retract @16:00:39.45 Elbow.reconfigure :tight @16:00:39.50 Elbow.seek :garage @16:00:42.39 Elbow.reconfigure :loose @16:00:42.49 Forearm.reconfigure :tight @16:00:42.54 Forearm.seek :garage @16:00:43.60 Hand.open @16:00:44.23 Forearm.reconfigure :loose @16:00:44.27 Forearm.seek :retract @16:00:45.26 Completed [initialPurge; da [50, 50] #da and wsr are run simultanelously] begun 14:09:57 @16:00:45.32 Asleep after completing DA_WCR_011024 assays @16:00:45.33 GoodNews.email "{1 => 4,\n 2 => 0,\n 3 => 0,\n 4 => 0,\n 5 => 0,\n 6 => 0,\n 7 => 0}\nCTD data unavailable\nCan@15:59:11, 25% flow, 22.7C, 10% humidity, 15.0psia, 13.691V, 0.550A, 57.912Ah, 7.53W\nSampled 50.0ml",Subject: "Asleep after completing DA_WCR_011024 assays" @16:00:49.29 upsync + ESPwhich DA_WCR_011024.rb + fn=DA_WCR_011024.rb + IFS=: + set /home/esp/esp2/mission . + fullPath=/home/esp/esp2/mission/DA_WCR_011024.rb + [ -f /home/esp/esp2/mission/DA_WCR_011024.rb ] + realpath /home/esp/esp2/mission/DA_WCR_011024.rb + return 0 + scriptPath=/home/esp/esp2local/mission/DA_WCR_011024.rb + exec ruby /home/esp/esp2/lib/esp.rb /home/esp/esp2local/mission/DA_WCR_011024.rb @20:04:44.53UTC18-Jan-24
ESP2 v3.42 I2C Gateway v3.20 for core bus @20:04:47.20 Configuration for short puck jake 1/11/21 by brent@mbari.org /home/esp/esp2/type/shallow/jake/configure.rb @20:04:50.89 Polling Can every 10:00 @20:04:50.91 Cleanly exited at 20:04:26UTC18-Jan-24 @20:04:50.96 Loading /home/esp/esp2local/mission/DA_WCR_011024.rb mission @20:04:51.02 GoodNews.email "Starting DA_WCR_011024 w/pucks in tube 2\n{}\nCTD data unavailable\nCan@20:04:50, 25% flow, 22.6C, 12% humidity, 15.0psia, 13.754V, 0.315A, 59.063Ah, 4.33W",Subject: "DA_WCR_011024 begun" @20:04:51.07
Preparing to sleep until + 00:00:05 @20:04:51.09 Asleep til + 00:00:05 @20:04:51.11 Continuing at 20:04 @20:04:51.40 Gate.power :core,:OFF @20:04:51.41 Gate.power :raw,:OFF @20:04:51.43 Shutdown @20:04:54.51 GoodNews.email "Then, in tube 2: initialPurge; da [50, 50] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_011024.rb:14\n{1 => 0,\n 2 => 4,\n 3 => 0,\n 4 => 0,\n 5 => 0,\n 6 => 0,\n 7 => 0}\nCTD data unavailable\nCan@20:04:51, 25% flow, 22.6C, 12% humidity, 15.0psia, 13.754V, 0.321A, 59.063Ah, 4.41W",Subject: "Asleep until 20:04" @20:04:55.91 upsync @20:04:55.99
Powering core bus @20:04:58.48 upsync @20:05:01.16
Gate.power :raw,:ON @20:05:02.23 CS.configure :default @20:05:02.28 PS.configure :default @20:05:02.32 SS.configure :default @20:05:02.37 CC.configure :default @20:05:02.42 PC.configure :default @20:05:02.49 Forearm.configure :loose @20:05:02.53 Elbow.configure :loose @20:05:02.60 SC.configure :loose @20:05:02.65 SE.configure :default @20:05:02.69 CH.configure :default @20:05:02.73 PH.configure :default @20:05:02.77 All dwarf controllers running firmware version 3.85 @20:05:02.88 Valve.reset @20:05:03.15 Intake.select :close @20:05:03.18 Exhaust.select :close @20:05:03.20 CTV.dial :air @20:05:03.27 PSV.dial :air @20:05:03.41 CSV.dial :waste1 @20:05:03.44
Forearm.home.seek :retract @20:05:03.88 SSV.dial :spit,avoiding: :exhaust @20:05:04.18 Hand.close @20:05:04.20
Elbow.reconfigure :loose @20:05:04.31 Elbow.jog 1500 @20:05:04.38 PS.home.seek 0ml @20:05:04.72
Elbow.reconfigure :loose @20:05:04.77 Elbow.home.seek :clear @20:05:04.82 CS.reconfigure :fast @20:05:04.89 SS.home.seek 0ml @20:05:04.98 CS.home.seek 0ml @20:05:08.04
SE.home.seek :down @20:05:08.66 SC.reconfigure :tight @20:05:08.70 SC.home.seek :initial @20:05:12.85 PS.stop @20:05:33.96
SC.configure :limp @20:05:34.02 Forearm.seek :retract @20:05:34.09 Elbow.seek :hammer @20:05:35.18 Forearm.reconfigure :tight @20:05:35.22 Forearm.seek retracted @20:05:35.52 PC.home.seek :open @20:05:35.54
CC.home.seek :open @20:05:38.99 Phase [initialPurge; da [50, 50] #da and wsr are run simultanelously] @20:05:39.00 Notice.email "At 20:05:38 began in tube 2: initialPurge; da [50, 50] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_011024.rb:14\n{1 => 0,\n 2 => 4,\n 3 => 0,\n 4 => 0,\n 5 => 0,\n 6 => 0,\n 7 => 0}\nCTD data unavailable\nCan@20:05:38, 25% flow, 22.6C, 11% humidity, 15.0psia, 13.734V, 0.290A, 59.068Ah, 3.99W",Subject: "Started DA_WCR_011024 phase initialPurge; da [50, 50] #da and wsr are run simultanelously" @20:05:39.02
Starting initialPurge @20:05:39.05 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @20:05:39.12 Hand.open @20:05:39.92 Elbow.reconfigure :tight @20:05:39.96 Elbow.seek FlushPuck::Garage @20:05:41.88 Elbow.reconfigure :loose @20:05:42.11 Forearm.reconfigure :loose @20:05:42.22 Forearm.seek :garage @20:05:43.24 Hand.close @20:05:43.35 upsync @20:05:43.92
Forearm.seek :retract @20:05:44.91 Elbow.reconfigure :tight @20:05:44.96 Elbow.seek CC @20:05:47.88 Elbow.reconfigure :loose @20:05:47.98 Forearm.reconfigure :tight @20:05:48.03 Forearm.seek CC @20:05:49.24 CC.seek :closed @20:05:53.87 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.43 @20:05:53.94 Hand.open @20:05:54.61 Forearm.reconfigure :loose @20:05:54.66 Forearm.seek :retract @20:05:55.64 Flushing the Collection Valve::Manifold @20:05:55.77 CSV.dial CSR @20:05:57.04 Pulling 5ml of CSR.flush @20:05:57.05 CSR.select :flush @20:05:57.17 CS.reconfigure :default @20:05:57.22 CS.seek 5ml @20:06:11.00 delay 5 seconds @20:06:16.02 CSR.select :air @20:06:16.59 CS.reconfigure :fast @20:06:16.64 CS.seek 7.5ml @20:06:23.50 CSV.dial :waste @20:06:24.78 CS.reconfigure :default @20:06:24.83 CS.seek 0ml @20:06:44.87 Purging external Intake @20:06:45.00 CSV.dial :flush @20:06:45.44 CS.reconfigure :fast @20:06:45.49 CS.seek 10ml @20:07:10.96 delay 5 seconds @20:07:15.99 CSV.dial CBV @20:07:16.03
CBV.dial CSV @20:07:16.75 CTV.dial :intake @20:07:17.53 Intake.select :open @20:07:17.64 CS.seek 0ml @20:07:41.21 Intake.select :close @20:07:41.31 CTV.dial :air @20:07:42.11 Flushing upper manifold @20:07:42.23 CSV.dial :flush @20:07:42.97 CS.seek 5ml @20:07:56.43 delay 5 seconds @20:08:01.45 CSV.dial :air @20:08:01.94 CS.seek 6.999ml @20:08:07.78 CSV.dial CBV @20:08:07.82
CBV.dial CSV @20:08:08.82 CTV.dial :air @20:08:08.91 CS.seek 0ml @20:08:25.71 delay 7 seconds @20:08:32.74 CS.seek 3ml @20:08:40.90 CSV.dial :waste @20:08:41.36 CS.seek 0ml @20:08:49.26 CSV.dial :flush @20:08:49.70 CS.seek 3ml @20:08:58.18 delay 5 seconds @20:09:03.20 CSV.dial :air @20:09:03.71 CS.seek 5ml @20:09:09.37 CSV.dial :air @20:09:09.45 CS.seek 0ml @20:09:21.65 delay 7 seconds @20:09:28.68 CS.seek 3ml @20:09:36.80 CSV.dial :waste @20:09:37.55 CS.seek 0ml @20:09:45.34 Garaging FlushPuck in Collection Clamp @20:09:45.41 Elbow.reconfigure :tight @20:09:45.45 Elbow.seek CC @20:09:45.77 Elbow.reconfigure :loose @20:09:45.87 Forearm.seek CC @20:09:46.93 Hand.close @20:09:47.55 CC.seek :open @20:09:52.60 Forearm.seek :retract @20:09:53.59 Elbow.reconfigure :tight @20:09:53.64 Elbow.seek :garage @20:09:56.55 Elbow.reconfigure :loose @20:09:56.65 Forearm.reconfigure :tight @20:09:56.70 Forearm.seek :garage @20:09:57.67 Hand.open @20:09:58.35 Forearm.reconfigure :loose @20:09:58.40 Forearm.seek :retract @20:09:59.42 da sampling at most 50ml, wcr at most 50ml @20:09:59.46 Loading sh1 puck from tube 2 into Collection Clamp @20:09:59.48 DAfans.email "Started da in tube 2 at 20:09:59UTC18-Jan-24\n{1 => 0,\n 2 => 4,\n 3 => 0,\n 4 => 0,\n 5 => 0,\n 6 => 0,\n 7 => 0}\nCTD data unavailable\nCan@20:05:38, 25% flow, 22.6C, 11% humidity, 15.0psia, 13.734V, 0.290A, 59.068Ah, 3.99W",Subject: "da sampling at most 50ml, wcr at most 50ml" @20:09:59.74
SC.reconfigure :tight @20:09:59.80 SC.seek 2 @20:10:03.36 upsync @20:10:06.56
SC.configure :limp @20:10:06.79 Elbow.reconfigure :tight @20:10:06.84 Elbow.seek 2 @20:10:08.78 Forearm.reconfigure :tight @20:10:08.83 Forearm.seek 2 + 450 counts @20:10:09.84 SE.reconfigure :pick @20:10:09.90 SE.seek :beamBreak @20:10:12.84 SE.configure :default @20:10:13.01 Elbow.seek 2 @20:10:13.19 Forearm.seek 2 + 300 counts @20:10:13.45 Forearm.reconfigure :loose @20:10:13.50 Hand.close @20:10:14.19 SE.seek :down @20:10:14.41
Forearm.seek :retract @20:10:15.33 Elbow.seek CC @20:10:16.84 SE.stop @20:10:16.97
Elbow.reconfigure :loose @20:10:17.07 Forearm.reconfigure :tight @20:10:17.12 Forearm.seek CC @20:10:18.39 CC.seek :closed @20:10:22.91 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.15 @20:10:22.92 Hand.open @20:10:23.60 Forearm.reconfigure :loose @20:10:23.65 Forearm.seek :retract @20:10:24.62 collectSample 50ml w/bubble point of 24psi @20:10:24.64 Priming 7ml @20:10:24.65 Intake.select :close @20:10:24.66 Exhaust.select :close @20:10:24.67 SS.hold @20:10:24.88 Calibrating pressure and temperature sensors @20:10:24.91 CTV.dial :air @20:10:24.99 SSV.dial :spit,avoiding: :exhaust @20:10:25.18 SS.reconfigure :fast @20:10:25.23 SS.seek 5ml @20:10:28.05 SSV.dial CBV @20:10:28.08
CBV.dial SSV @20:10:29.41 delay 3.8 seconds @20:10:33.34 Sensor limits: Intake max 117.8psia; Outlet max 111.3psia @20:10:33.35 SSV.dial :spit @20:10:35.12 SS.seek 0ml @20:10:37.96 SSV.dial CBV @20:10:39.27 CTV.dial :intake @20:10:40.06 SSV.dial CBV @20:10:40.09
CBV.dial SSV @20:10:40.16 Allowing 40s for 7.0ml of remaining 7.0ml @20:10:40.17 Intake.select :open @20:10:42.33 Intake @14.9psia, vacuum from 9.4 to 12.4psi @20:10:42.35 SS.reconfigure :inhaling 2.20ml/s @20:10:42.40 SS.seek 7ml @20:10:49.36 Stroke completed -- waiting for pressure to equalize ... @20:10:49.37 SS.reconfigure :exhale @20:10:49.42 SS.hold @20:10:52.52 Intake.select :close @20:10:52.67 SSV.dial :exhaust @20:10:53.44 Exhaust.select :open @20:10:53.55 SS.seek 0ml @20:10:57.72 Exhaust.select :close @20:10:57.83 Sampling 50.0ml @20:10:57.84 Intake.select :close @20:10:57.86 Exhaust.select :close @20:10:57.87 SS.reconfigure :default @20:10:57.91 SS.hold @20:10:58.11 CTV.dial :intake @20:10:58.29 SSV.dial CBV @20:10:58.32
CBV.dial SSV @20:10:59.08 Allowing 130s for 25.0ml of remaining 50.0ml @20:10:59.10 Intake.select :open @20:11:01.26 Intake @15.7psia, vacuum from 10.0 to 13.0psi @20:11:01.28 SS.reconfigure :inhaling 2.20ml/s @20:11:01.32 SS.seek 25ml @20:11:11.90 Vacuum too high -- waiting up to 40 seconds @20:11:11.91 SS.reconfigure :exhale @20:11:11.97 SS.hold @20:11:12.64 Intake @12.8psia, vacuum from 7.3 to 10.3psi @20:11:12.66 SS.reconfigure :inhaling 1.76ml/s @20:11:12.71 SS.seek 25ml @20:11:18.20 Stroke completed -- waiting for pressure to equalize ... @20:11:18.21 SS.reconfigure :exhale @20:11:18.26 SS.hold @20:11:23.78 Intake.select :close @20:11:23.94 SSV.dial :exhaust @20:11:24.69 Exhaust.select :open @20:11:24.80 SS.seek 0ml @20:11:33.69 Exhaust.select :close @20:11:33.80 SSV.dial CBV @20:11:33.82
CBV.dial SSV @20:11:34.60 Allowing 130s for 25.0ml of remaining 25.0ml @20:11:34.61 Intake.select :open @20:11:37.19 Intake @15.0psia, vacuum from 9.5 to 12.5psi @20:11:37.21 SS.reconfigure :inhaling 1.76ml/s @20:11:37.25 SS.seek 25ml @20:11:43.21 Vacuum too high -- waiting up to 40 seconds @20:11:43.23 SS.reconfigure :exhale @20:11:43.28 SS.hold @20:12:23.75 SS.reconfigure :default @20:12:23.80 SS.hold @20:12:23.87 Intake.select :close @20:12:24.02 ShallowSampler::Clogged in MAIN -- Intake @14.7psia, & Outlet @2.8psia -- no progress after 30.1ml @20:12:24.03 SSV.dial :exhaust @20:12:24.80 Exhaust.select :open @20:12:24.91 SS.reconfigure :exhale @20:12:24.96 SS.seek 0ml @20:12:28.53 Exhaust.select :close @20:12:28.64 Sampled 30.1ml @20:12:28.67 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) Intake.select :close @20:12:28.74 CSV.dial :waste @20:12:28.86 SSV.dial CBV,avoiding: :exhaust @20:12:28.90
CBV.dial SSV @20:12:28.96 CS.seek 0ml @20:12:29.03 CSV.dial :air @20:12:29.76 CS.seek 10ml @20:12:30.77
CTV.dial :air @20:12:31.54 delay 15 seconds @20:12:46.56 SSV.dial :spit,avoiding: :exhaust @20:12:47.87 CTV.dial CSV,avoiding: :intake @20:12:47.91
SS.seek 0ml @20:12:48.03 SSV.dial CBV,avoiding: :exhaust @20:12:54.46 SSV.dial between CBV and plug @20:12:54.94 CBV.dial between SSV and waste2 @20:12:55.33 CTV.dial CSV,avoiding: :air @20:12:55.36
CSV.dial CTV @20:12:55.90 CS.reconfigure :pressurize @20:12:55.95 CS.seek 0ml @20:13:17.68 BubblePoint: Intake @24.3psia, Outlet @-8.5psia @20:13:17.69 CBV.dial SSV @20:13:18.02 SS.reconfigure :default @20:13:18.07 SS.seek 10ml @20:13:23.75 delay 15 seconds @20:13:38.88 Evacuated: Intake @22.2psia, Outlet @15.3psia @20:13:38.89 SSV.dial :spit,avoiding: :exhaust @20:13:39.94 SS.reconfigure :exhale @20:13:39.99 SS.seek 0ml @20:13:45.04 SS.reconfigure :fast @20:13:45.09 SS.seek 5ml @20:13:47.88 SSV.dial CBV,avoiding: :exhaust @20:13:47.91
CBV.dial SSV @20:13:49.25 CTV.dial :air,avoiding: :intake @20:13:50.55 CSV.dial CTV @20:13:50.59
SS.seek 10ml @20:13:51.81 CSV.dial :air @20:13:52.24 CS.reconfigure :fast @20:13:52.29 CS.seek 0ml @20:13:53.10
SSV.dial :spit,avoiding: :exhaust @20:13:54.41 SS.reconfigure :exhale @20:13:54.89 SS.seek 0ml @20:13:57.14 CS.stop @20:14:00.21
SS.reconfigure :fast @20:14:00.26 SS.seek 5ml @20:14:03.10 SSV.dial CBV,avoiding: :exhaust @20:14:03.13
CBV.dial SSV @20:14:05.48 SSV.dial :spit,avoiding: :exhaust @20:14:06.80 SS.reconfigure :default @20:14:06.85 SS.seek 0ml @20:14:10.12 SS.stop @20:14:10.16 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @20:14:10.17 Intake.select :close @20:14:10.25 CSV.dial :waste @20:14:10.37 SSV.dial CBV,avoiding: :exhaust @20:14:10.40
CBV.dial SSV @20:14:11.01 CS.seek 0ml @20:14:11.08 CSV.dial :air @20:14:11.74
CTV.dial :air @20:14:11.84 delay 15 seconds @20:14:11.86 CS.seek 10ml @20:14:26.86
SSV.dial :spit,avoiding: :exhaust @20:14:28.18 CTV.dial CSV,avoiding: :intake @20:14:28.22
SS.reconfigure :exhale @20:14:28.31 SS.seek 0ml @20:14:28.71 SSV.dial CBV,avoiding: :exhaust @20:14:36.52 SSV.dial between CBV and plug @20:14:37.00 CBV.dial between SSV and waste2 @20:14:37.39 CSV.dial between air and CTV @20:14:37.70 CS.seek 7.969ml @20:14:43.18 CTV.dial CSV,avoiding: :air @20:14:43.22
CSV.dial CTV @20:14:43.59 CS.reconfigure :pressurize @20:14:43.63 CS.seek 0ml @20:14:59.84 BubblePoint: Intake @24.1psia, Outlet @-0.4psia @20:14:59.85 CBV.dial SSV @20:15:01.18 SS.reconfigure :default @20:15:01.23 SS.seek 10ml @20:15:06.98 delay 15 seconds @20:15:22.12 Evacuated: Intake @22.6psia, Outlet @15.2psia @20:15:22.13 SSV.dial :spit,avoiding: :exhaust @20:15:23.18 SS.reconfigure :exhale @20:15:23.22 SS.seek 0ml @20:15:28.24 SS.reconfigure :fast @20:15:28.29 SS.seek 5ml @20:15:31.19 SSV.dial CBV,avoiding: :exhaust @20:15:31.23
CBV.dial SSV @20:15:32.55 CTV.dial :air,avoiding: :intake @20:15:33.85 CSV.dial CTV @20:15:33.90
SS.seek 10ml @20:15:35.05 CSV.dial :air @20:15:35.57 CS.reconfigure :fast @20:15:35.62 CS.seek 0ml @20:15:36.34
SSV.dial :spit,avoiding: :exhaust @20:15:37.66 SS.reconfigure :exhale @20:15:37.71 SS.seek 0ml @20:15:40.67 CS.stop @20:15:42.99
SS.reconfigure :fast @20:15:43.04 SS.seek 5ml @20:15:45.89 SSV.dial CBV,avoiding: :exhaust @20:15:45.91
CBV.dial SSV @20:15:48.26 SSV.dial :spit,avoiding: :exhaust @20:15:49.56 SS.reconfigure :default @20:15:49.61 SS.seek 0ml @20:15:52.95 SS.stop @20:15:52.99 Lysing: Adding 2.0ml AqMeOH @20:15:53.00 CBV.dial between CSV and waste1,avoiding: :DBV @20:15:54.49 CTV.dial CSV @20:15:55.78 CSV.dial CSR @20:15:56.57 Pulling 1ml of CSR.AqMeOH @20:15:56.58 CSR.select :AqMeOH @20:15:56.70 CS.reconfigure :default @20:15:56.75 CS.seek 1ml @20:16:00.08 delay 5 seconds @20:16:05.10 CSR.select :air @20:16:05.27 CS.seek 6ml @20:16:18.99 delay 2 seconds @20:16:21.00 CSV.dial :waste2 @20:16:21.44 CS.reconfigure :fast @20:16:21.49 CS.seek 0ml @20:16:36.01 CSV.dial CSR @20:16:36.47 Pulling 2ml of CSR.AqMeOH @20:16:36.48 CSR.select :AqMeOH @20:16:36.59 CS.reconfigure :default @20:16:36.64 CS.seek 2ml @20:16:42.64 delay 5 seconds @20:16:47.66 CSR.select :air @20:16:47.83 CS.seek 5ml @20:16:56.42 delay 2 seconds @20:16:58.44 CSV.dial CTV @20:16:58.88 CS.seek 1ml @20:17:10.00 Before lysing Collection Puck Heater at 22.3C/25.4psia @20:17:10.02 Heating Puck.from 2, :sh1 in Collection Clamp to 70C for 10:00 @20:17:10.04 CH.seek 70C,8:00 @20:18:59.55 CH.hold 10:00,70C @20:18:59.73 Begun lysing Collection Puck Heater at 68.1C/30.7psia @20:28:59.75 Ended lysing Collection Puck Heater at 70C/29.3psia @20:28:59.77 CS.seek 0ml @20:29:03.20 CTV.dial between spare and air,avoiding: :intake @20:29:04.21 CSV.dial :air @20:29:04.66 CS.seek 5ml @20:29:18.47 CSV.dial CTV @20:29:18.93 delay 2 seconds @20:29:20.94 CSV.dial :air @20:29:21.39 delay 2 seconds @20:29:23.41 CSV.dial CTV @20:29:23.86 delay 2 seconds @20:29:25.88 CSV.dial :air @20:29:26.33 delay 2 seconds @20:29:28.35 CS.seek 0ml @20:29:41.93 Puck @58.7psia after final lysis pressurization @20:29:41.94 Recovering Lysate into Collection syringe @20:29:41.95 CSV.dial CBV @20:29:42.94 CBV.dial CSV @20:29:43.31 CS.seek 5.001ml @20:29:57.10 delay 5 seconds @20:30:02.11 CTV.dial :air @20:30:02.64 CS.seek 7.001ml @20:30:08.75 delay 5 seconds @20:30:13.77 CSV.dial :air2 @20:30:14.66 CS.seek 10ml @20:30:23.13 CSV.dial between air and CTV,avoiding: CBV @20:30:24.29 Lysing Complete @20:30:24.35 Storing sh1 puck in Collection Clamp to tube 1 @20:30:24.53 SC.reconfigure :tight @20:30:24.58 SC.seek 1 @20:30:28.15 SC.configure :limp @20:30:28.32 SE.reconfigure :place @20:30:28.41
Elbow.reconfigure :tight @20:30:28.45 SE.seek :beamBreak @20:30:28.47
Elbow.seek CC @20:30:28.78 Elbow.reconfigure :loose @20:30:28.88 Forearm.seek CC @20:30:29.85 Hand.close @20:30:30.40 CC.seek :open @20:30:31.30 SE.configure :default @20:30:31.36 SE.seek :place @20:30:35.14
Forearm.seek :retract @20:30:36.18 Elbow.reconfigure :tight @20:30:36.23 Elbow.seek 1 @20:30:37.95 Elbow.reconfigure :limp @20:30:38.00 Forearm.seek 1 @20:30:38.95 Elbow.hold @20:30:39.01 Elbow.reconfigure :tight @20:30:39.07 Hand.open @20:30:39.77 Forearm.force -20 @20:30:39.81 Forearm.seek 1 + 317 counts @20:30:40.11 Forearm.reconfigure :tight @20:30:40.16 Forearm.seek 1 + 450 counts @20:30:40.40 SE.seek :down @20:30:41.53
Forearm.reconfigure :loose @20:30:41.58 Forearm.seek :retract @20:30:43.09 SE.stop @20:30:43.58
Tranferring DA @20:30:43.59 Beginning samplePassC @20:30:43.61 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @20:30:43.80 Elbow.seek FlushPuck::Garage @20:30:45.58 Elbow.reconfigure :loose @20:30:45.68 Forearm.seek :garage @20:30:46.62 Hand.close @20:30:47.39 Forearm.seek :retract @20:30:48.40 Elbow.reconfigure :tight @20:30:48.45 Elbow.seek CC @20:30:51.35 Elbow.reconfigure :loose @20:30:51.45 Forearm.reconfigure :tight @20:30:51.50 Forearm.seek CC @20:30:52.73 CC.seek :closed @20:30:57.26 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.39 @20:30:57.32 Hand.open @20:30:57.96 Forearm.reconfigure :loose @20:30:58.01 Forearm.seek :retract @20:30:58.95 PSV.dial PSR @20:31:00.43 Pulling 1.5ml of PSR.flush @20:31:00.44 PSR.select :flush @20:31:00.56 PS.seek 1.5ml @20:31:16.67 delay 5 seconds @20:31:21.69 PSV.dial :waste1 @20:31:22.16 PS.seek 0ml @20:31:38.26 PSV.dial PSR @20:31:38.72 PSR.select :air @20:31:38.83 PS.seek 2.5ml @20:32:05.17 PRV.dial CBV,avoiding: :ab2 @20:32:05.20
CBV.dial PRV @20:32:07.37 PSV.dial :mixloop @20:32:08.41 CTV.dial :air,avoiding: :intake @20:32:08.58 PTV.dial :PRVmixing @20:32:09.89 PS.seek 0ml @20:32:36.23 PSV.dial :air @20:32:36.81 PS.seek 2.5ml @20:33:03.26 PSV.dial :mixloop @20:33:03.72 PS.seek 0ml @20:33:30.07 PSV.dial :air @20:33:30.55 PS.seek 2.5ml @20:33:56.92 PSV.dial :mixloop @20:33:57.39 PS.seek 0ml @20:34:23.79 Garaging FlushPuck in Collection Clamp @20:34:23.86 Elbow.reconfigure :tight @20:34:23.91 Elbow.seek CC @20:34:24.22 Elbow.reconfigure :loose @20:34:24.32 Forearm.seek CC @20:34:25.33 Hand.close @20:34:25.90 CC.seek :open @20:34:30.55 Forearm.seek :retract @20:34:31.54 Elbow.reconfigure :tight @20:34:31.59 Elbow.seek :garage @20:34:34.50 Elbow.reconfigure :loose @20:34:34.60 Forearm.reconfigure :tight @20:34:34.65 Forearm.seek :garage @20:34:35.68 Hand.open @20:34:36.33 Forearm.reconfigure :loose @20:34:36.38 Forearm.seek :retract @20:34:37.36 PSV.dial :mixloop @20:34:37.45 PTV.dial :PRVmixing @20:34:37.53 PRV.dial CBV @20:34:37.56
CBV.dial PRV @20:34:37.68 CTV.dial :air,avoiding: :intake @20:34:37.70 Loading lyfil puck from tube 2 into Collection Clamp @20:34:37.94 SC.reconfigure :tight @20:34:37.98 SC.seek 2 @20:34:42.02 SC.configure :limp @20:34:42.24 Elbow.reconfigure :tight @20:34:42.28 Elbow.seek 2 @20:34:44.21 Forearm.reconfigure :tight @20:34:44.25 Forearm.seek 2 + 450 counts @20:34:45.48 SE.reconfigure :pick @20:34:45.54 SE.seek :beamBreak @20:34:48.48 SE.configure :default @20:34:48.63 Elbow.seek 2 @20:34:48.82 Forearm.seek 2 + 300 counts @20:34:49.01 Forearm.reconfigure :loose @20:34:49.05 Hand.close @20:34:49.66 SE.seek :down @20:34:49.86
Forearm.seek :retract @20:34:50.82 Elbow.seek CC @20:34:52.34 SE.stop @20:34:52.46
Elbow.reconfigure :loose @20:34:52.56 Forearm.reconfigure :tight @20:34:52.61 Forearm.seek CC @20:34:53.94 CC.seek :closed @20:34:58.88 Collection Clamp onSpring/offSpring for 13/27 samples, on/off torque 2.13 @20:34:58.96 Hand.open @20:34:59.58 Forearm.reconfigure :loose @20:34:59.63 Forearm.seek :retract @20:35:00.62 CTV.dial CSV,avoiding: :intake @20:35:01.95 CSV.dial CTV @20:35:02.29 PS.seek 2.5ml @20:35:28.72 CS.seek 4.499ml @20:35:43.59 delay 8 seconds @20:35:51.61 PSV.dial :air @20:35:52.07 delay 2 seconds @20:35:54.09 PSV.dial :waste2 @20:35:54.83 PS.seek 0ml @20:36:21.19 CBV.dial between PRV and waste1 @20:36:21.53 CSV.dial :air @20:36:21.99 CS.seek 10ml @20:36:36.90 CSV.dial CTV @20:36:37.36 CS.seek 0ml @20:37:03.71 CBV.dial :waste1 @20:37:04.01 delay 5 seconds @20:37:09.03 CTV.dial :air,avoiding: :intake @20:37:10.43 Storing lyfil puck in Collection Clamp to tube 1 @20:37:10.61 SC.reconfigure :tight @20:37:10.66 SC.seek 1 @20:37:14.30 SC.configure :limp @20:37:14.52 Elbow.reconfigure :tight @20:37:14.57 Elbow.seek 1 @20:37:16.58 Forearm.reconfigure :tight @20:37:16.62 Forearm.seek 1 + 450 counts @20:37:17.64 SE.reconfigure :pick @20:37:17.70 SE.seek :beamBreak @20:37:20.72 SE.configure :default @20:37:20.88 Elbow.reconfigure :limp @20:37:20.93 Forearm.reconfigure :loose @20:37:21.41 Forearm.seek 1 @20:37:21.69 Elbow.hold @20:37:21.76 Elbow.reconfigure :tight @20:37:21.86 SE.seek 0.15 Pucks below Top Plate @20:37:22.27 Forearm.force -20 @20:37:22.31 Forearm.seek 1 + 317 counts @20:37:22.61 Forearm.reconfigure :tight @20:37:22.65 Forearm.seek 1 + 450 counts @20:37:22.85 SE.seek 1.15 Pucks below Top Plate @20:37:23.36 Forearm.reconfigure :loose @20:37:23.41 Forearm.seek :retract @20:37:24.34 Elbow.seek CC @20:37:25.91 Elbow.reconfigure :loose @20:37:26.01 Forearm.seek CC @20:37:27.00 Hand.close @20:37:27.65 CC.seek :open @20:37:32.38 Forearm.seek :retract @20:37:33.38 Elbow.reconfigure :tight @20:37:33.43 Elbow.seek 1 @20:37:35.18 Elbow.reconfigure :limp @20:37:35.23 Forearm.seek 1 @20:37:36.22 Elbow.hold @20:37:36.29 Elbow.reconfigure :tight @20:37:36.33 Hand.open @20:37:37.01 Forearm.force -20 @20:37:37.05 Forearm.seek 1 + 304 counts @20:37:37.91 Forearm.reconfigure :tight @20:37:37.96 Forearm.seek 1 + 450 counts @20:37:38.30 SE.seek :down @20:37:39.44
Forearm.reconfigure :loose @20:37:39.49 Forearm.seek :retract @20:37:40.92 SE.stop @20:37:40.96
PSV.dial :air @20:37:41.72 PS.seek 2.5ml @20:38:08.13 delay 1 second @20:38:09.15 PSV.dial :mixloop @20:38:09.71 delay 1 second @20:38:10.73 PSV.dial :air @20:38:11.18 delay 1 second @20:38:12.19 PSV.dial :mixloop @20:38:12.66 delay 1 second @20:38:13.68 PSV.dial :air @20:38:14.15 delay 1 second @20:38:15.17 PS.seek 0ml @20:38:41.60 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @20:38:41.78 Elbow.seek FlushPuck::Garage @20:38:43.57 Elbow.reconfigure :loose @20:38:43.67 Forearm.seek :garage @20:38:44.74 Hand.close @20:38:45.52 Forearm.seek :retract @20:38:46.52 Elbow.reconfigure :tight @20:38:46.57 Elbow.seek PC @20:38:47.84 Elbow.reconfigure :loose @20:38:47.94 Forearm.reconfigure :tight @20:38:47.99 Forearm.seek PC @20:38:49.24 PC.seek :closed @20:38:53.91 Processing Clamp onSpring/offSpring for 16/26 samples, on/off torque 1.61 @20:38:53.98 Hand.open @20:38:54.71 Forearm.reconfigure :loose @20:38:54.75 Forearm.seek :retract @20:38:55.70 PRV.dial :air @20:38:56.16 PSV.dial :mixloop @20:38:56.70 PS.seek 0.3ml @20:39:00.53 PSV.dial PSR @20:39:01.58 Pulling 0.4ml of PSR.air @20:39:01.59 PSR.select :air @20:39:01.62 PS.seek 0.7ml @20:39:06.60 delay 3 seconds @20:39:09.61 PSR.select :air @20:39:09.64 PS.seek 2.5ml @20:39:28.91 delay 3 seconds @20:39:31.93 PSV.dial :mixloop @20:39:33.10 PTV.dial :mixing @20:39:33.83 PBV.dial :waste2 @20:39:34.79 PS.seek 0ml @20:40:01.13 Garaging FlushPuck in Processing Clamp @20:40:01.20 Elbow.reconfigure :tight @20:40:01.24 Elbow.seek PC @20:40:01.57 Elbow.reconfigure :loose @20:40:01.67 Forearm.seek PC @20:40:02.63 Hand.close @20:40:03.27 PC.seek :open @20:40:08.00 Forearm.seek :retract @20:40:08.94 Elbow.reconfigure :tight @20:40:08.99 Elbow.seek :garage @20:40:10.29 Elbow.reconfigure :loose @20:40:10.39 Forearm.reconfigure :tight @20:40:10.44 Forearm.seek :garage @20:40:11.45 Hand.open @20:40:12.08 Forearm.reconfigure :loose @20:40:12.12 Forearm.seek :retract @20:40:13.55 Loading da puck from tube 2 into Processing Clamp @20:40:13.79 SC.reconfigure :tight @20:40:13.84 SC.seek 2 @20:40:17.46 SC.configure :limp @20:40:17.68 Elbow.reconfigure :tight @20:40:17.73 Elbow.seek 2 @20:40:19.64 Forearm.reconfigure :tight @20:40:19.69 Forearm.seek 2 + 450 counts @20:40:20.69 SE.reconfigure :pick @20:40:20.74 SE.seek :beamBreak @20:40:23.78 SE.configure :default @20:40:23.93 Elbow.seek 2 @20:40:24.23 Forearm.seek 2 + 300 counts @20:40:24.53 Forearm.reconfigure :loose @20:40:24.57 Hand.close @20:40:25.15 SE.seek :down @20:40:25.35
Forearm.seek :retract @20:40:26.27 Elbow.seek PC @20:40:27.85 SE.stop @20:40:28.74
Elbow.reconfigure :loose @20:40:28.84 Forearm.reconfigure :tight @20:40:28.89 Forearm.seek PC @20:40:30.17 PC.seek :closed @20:40:34.80 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.63 @20:40:34.81 Hand.open @20:40:35.46 Forearm.reconfigure :loose @20:40:35.52 Forearm.seek :retract @20:40:36.46 Heating Puck.from 2, :da in Processing Clamp to 28C for 2:00:00 @20:40:36.48 PH.seek 28C,5:00 @20:41:05.06 PH.hold 2:00:00,28C @20:41:05.10 delay 1:30 @20:42:35.16 PSR.select :air @20:42:35.17 PSV.dial PSR @20:42:36.20 Pulling 0.5ml of PSR.wash @20:42:36.21 PSR.select :wash @20:42:36.33 PS.seek 0.5ml @20:42:42.25 delay 5 seconds @20:42:47.27 PSR.select :air @20:42:47.39 PS.seek 2ml @20:43:03.53 PTV.dial :puck @20:43:04.27 PSV.dial PTV @20:43:05.14 PBV.dial :waste1 @20:43:06.51 PS.seek 1.5ml @20:43:12.41 delay 3 seconds @20:43:15.42 PBV.dial between PSV and waste1 @20:43:15.79 PS.seek 0ml @20:43:32.10 delay 5:00 @20:48:32.12 recoverPSRreagent08 @20:48:32.13 PTV.dial :puck @20:48:32.63 PBV.dial :waste1 @20:48:33.01 delay 30 seconds @20:49:03.03 PBV.dial between PSV and waste1 @20:49:03.41 PSR.select :air @20:49:03.43 PSV.dial PSR @20:49:04.17 PS.reconfigure :fast @20:49:04.22 PS.seek 2.5ml @20:49:27.87 PSV.dial PTV @20:49:28.62 delay 5 seconds @20:49:33.64 PSV.dial PSR @20:49:34.38 delay 5 seconds @20:49:39.40 PSV.dial PTV @20:49:40.18 delay 5 seconds @20:49:45.20 PSV.dial :air @20:49:45.95 PS.seek 0ml @20:50:09.09 PTV.dial :PRVmixing,via: PRV @20:50:10.41 PSV.dial :mixloop @20:50:10.88 PRV.dial :ab1 @20:50:11.64 PS.reconfigure :slow @20:50:11.68 PS.seek 0.15ml @20:50:15.40 delay 2 seconds @20:50:17.42 CBV.dial PRV @20:50:17.45
PRV.dial CBV @20:50:18.00 PS.seek 0.34ml @20:50:22.43 delay 2 seconds @20:50:24.45 CBV.dial between PRV and PSV @20:50:24.76 PRV.dial :air @20:50:25.22 PS.seek 0.9ml @20:50:37.11 PRV.dial :waste2 @20:50:37.86 PS.seek 0.5ml @20:50:46.49 addPMLReagent08 @20:50:46.50 PSV.dial :air @20:50:47.07 PS.reconfigure :default @20:50:47.12 PS.seek 2ml @20:51:03.24 PSV.dial :PML @20:51:03.69 PTV.dial :mixing @20:51:04.43 PBV.dial :waste1 @20:51:04.87 PS.seek 1.85ml @20:51:07.24 PBV.dial between PSV and waste1 @20:51:07.60 PS.seek 0ml @20:51:27.30 delay 2 seconds @20:51:29.32 Beginning samplePassC @20:51:29.33 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @20:51:29.51 Elbow.reconfigure :tight @20:51:29.56 Elbow.seek FlushPuck::Garage @20:51:30.91 Elbow.reconfigure :loose @20:51:31.44 Forearm.seek :garage @20:51:32.45 Hand.close @20:51:33.13 Forearm.seek :retract @20:51:34.12 Elbow.reconfigure :tight @20:51:34.17 Elbow.seek CC @20:51:37.03 Elbow.reconfigure :loose @20:51:37.13 Forearm.reconfigure :tight @20:51:37.18 Forearm.seek CC @20:51:38.52 CC.seek :closed @20:51:43.05 Collection Clamp onSpring/offSpring for 13/27 samples, on/off torque 2.32 @20:51:43.12 Hand.open @20:51:43.82 Forearm.reconfigure :loose @20:51:43.86 Forearm.seek :retract @20:51:44.80 PSV.dial PSR @20:51:45.85 Pulling 1.5ml of PSR.flush @20:51:45.86 PSR.select :flush @20:51:45.98 PS.seek 1.5ml @20:52:02.14 delay 5 seconds @20:52:07.16 PSV.dial :waste1 @20:52:07.63 PS.seek 0ml @20:52:23.74 PSV.dial PSR @20:52:24.21 PSR.select :air @20:52:24.32 PS.seek 2.5ml @20:52:50.84 PRV.dial CBV,avoiding: :ab2 @20:52:50.86
CBV.dial PRV @20:52:52.49 PSV.dial :mixloop @20:52:53.52 CTV.dial :air,avoiding: :intake @20:52:53.60 PTV.dial :PRVmixing @20:52:54.81 PS.seek 0ml @20:53:21.17 PSV.dial :air @20:53:21.76 PS.seek 2.5ml @20:53:48.30 PSV.dial :mixloop @20:53:48.76 PS.seek 0ml @20:54:15.11 PSV.dial :air @20:54:15.59 PS.seek 2.5ml @20:54:41.98 PSV.dial :mixloop @20:54:42.46 PS.seek 0ml @20:55:08.80 Garaging FlushPuck in Collection Clamp @20:55:08.87 Elbow.reconfigure :tight @20:55:08.91 Elbow.seek CC @20:55:09.20 Elbow.reconfigure :loose @20:55:09.30 Forearm.seek CC @20:55:10.32 Hand.close @20:55:10.88 CC.seek :open @20:55:15.50 Forearm.seek :retract @20:55:16.49 Elbow.reconfigure :tight @20:55:16.54 Elbow.seek :garage @20:55:19.42 Elbow.reconfigure :loose @20:55:19.52 Forearm.reconfigure :tight @20:55:19.57 Forearm.seek :garage @20:55:20.61 Hand.open @20:55:21.31 Forearm.reconfigure :loose @20:55:21.36 Forearm.seek :retract @20:55:22.35 delayUntil "20:59:29.31" @20:55:22.38 Whole Cell Archive sampling 50ml @20:55:22.39 Loading wcr puck from tube 2 into Collection Clamp @20:55:22.69 SC.reconfigure :tight @20:55:22.74 SC.seek 2 @20:55:23.01 SC.configure :limp @20:55:23.24 Elbow.reconfigure :tight @20:55:23.73 Elbow.seek 2 @20:55:25.63 Forearm.reconfigure :tight @20:55:25.69 Forearm.seek 2 + 450 counts @20:55:26.72 SE.reconfigure :pick @20:55:26.79 SE.seek :beamBreak @20:55:29.85 SE.configure :default @20:55:30.01 Elbow.seek 2 @20:55:30.21 Forearm.seek 2 + 300 counts @20:55:30.43 Forearm.reconfigure :loose @20:55:30.47 Hand.close @20:55:31.14 Puck::Warning in WCR -- Loaded Last remaining Puck (from Tube 2) @20:55:31.16 SE.seek :down @20:55:31.36 Forearm.seek :retract @20:55:32.32 Elbow.seek CC @20:55:33.97 SE.stop @20:55:34.01 Elbow.reconfigure :loose @20:55:34.15 Forearm.reconfigure :tight @20:55:34.20 Forearm.seek CC @20:55:35.44 CC.seek :closed @20:55:39.97 Collection Clamp onSpring/offSpring for 13/27 samples, on/off torque 2.25 @20:55:39.99 Hand.open @20:55:40.76 Forearm.reconfigure :loose @20:55:40.81 Forearm.seek :retract @20:55:41.79 collectSample 50ml w/bubble point of 35psi @20:55:41.80 Priming 7ml @20:55:41.81 Intake.select :close @20:55:41.82 Exhaust.select :close @20:55:41.84 SS.hold @20:55:42.05 Calibrating pressure sensors @20:55:42.06 CTV.dial :air @20:55:42.13 SSV.dial :spit,avoiding: :exhaust @20:55:42.22 SS.reconfigure :fast @20:55:42.27 SS.seek 5ml @20:55:44.97 SSV.dial CBV @20:55:44.99 CBV.dial SSV @20:55:46.33 delay 3.8 seconds @20:55:50.26 Sensor limits: Intake max 118.2psia; Outlet max 111.3psia @20:55:50.27 SSV.dial :spit @20:55:51.63 SS.seek 0ml @20:55:54.45 SSV.dial CBV @20:55:55.75 CTV.dial :intake @20:55:56.49 SSV.dial CBV @20:55:56.52 CBV.dial SSV @20:55:56.60 Allowing 40s for 7.0ml of remaining 7.0ml @20:55:56.61 Intake.select :open @20:55:58.78 Intake @14.9psia, vacuum from 9.4 to 12.4psi @20:55:58.80 SS.reconfigure :inhaling 2.20ml/s @20:55:58.85 SS.seek 7ml @20:56:05.44 Stroke completed -- waiting for pressure to equalize ... @20:56:05.46 SS.reconfigure :exhale @20:56:05.51 SS.hold @20:56:05.86 Intake.select :close @20:56:06.02 SSV.dial :exhaust @20:56:06.78 Exhaust.select :open @20:56:06.89 SS.seek 0ml @20:56:10.85 Exhaust.select :close @20:56:10.97 Sampling 50.0ml @20:56:10.98 Intake.select :close @20:56:10.99 Exhaust.select :close @20:56:11.01 SS.reconfigure :default @20:56:11.05 SS.hold @20:56:11.27 CTV.dial :intake @20:56:11.51 SSV.dial CBV @20:56:11.54 CBV.dial SSV @20:56:12.76 Allowing 130s for 25.0ml of remaining 50.0ml @20:56:12.77 Intake.select :open @20:56:14.94 Intake @14.6psia, vacuum from 9.1 to 12.1psi @20:56:14.96 SS.reconfigure :inhaling 2.20ml/s @20:56:15.01 SS.seek 25ml @20:56:29.82 Stroke completed -- waiting for pressure to equalize ... @20:56:29.83 SS.reconfigure :exhale @20:56:29.88 SS.hold @20:56:30.23 Intake.select :close @20:56:30.39 SSV.dial :exhaust @20:56:31.15 Exhaust.select :open @20:56:31.27 SS.seek 0ml @20:56:40.10 Exhaust.select :close @20:56:40.22 SSV.dial CBV @20:56:40.25 CBV.dial SSV @20:56:41.03 Allowing 130s for 25.0ml of remaining 25.0ml @20:56:41.04 Intake.select :open @20:56:43.21 Intake @15.0psia, vacuum from 9.5 to 12.5psi @20:56:43.22 SS.reconfigure :inhaling 2.20ml/s @20:56:43.27 SS.seek 25ml @20:56:58.73 Stroke completed -- waiting for pressure to equalize ... @20:56:58.74 SS.reconfigure :exhale @20:56:58.79 SS.hold @20:56:59.15 Intake.select :close @20:56:59.31 SSV.dial :exhaust @20:57:00.07 Exhaust.select :open @20:57:00.19 SS.seek 0ml @20:57:08.65 Exhaust.select :close @20:57:08.76 Sampled 50.0ml @20:57:08.78 Drying puck before fixing for 5:00 @20:57:08.79 CBV.dial :waste2b @20:57:09.54 delay 1:00 @20:58:09.57 CSV.dial :air @20:58:10.02 CS.reconfigure :fast @20:58:10.08 CS.seek 10ml @20:58:34.54 Pumping wcr puck from tube 2 up to 35psi... @20:58:34.56 CBV.dial SSV @20:58:35.82 CBV.dial between CSV and waste2b @20:58:36.76 CSV.dial between air and CTV @20:58:37.09 CS.seek 7.33ml @20:58:44.04 CTV.dial CSV,avoiding: :air @20:58:44.07 CSV.dial CTV @20:58:44.90 CS.reconfigure :pressurize @20:58:44.96 CS.seek 0ml @20:59:04.31 CSV.dial :air @20:59:04.77 CS.reconfigure :fast @20:59:04.83 CS.seek 10ml @20:59:29.28 CSV.dial between air and CTV @20:59:29.34 recoverPRVreagent08 @20:59:29.35 PTV.dial :mixing @20:59:29.62 CS.seek 7.33ml @20:59:30.10 PBV.dial :waste1 @20:59:30.48 delay 30 seconds @20:59:36.45 CTV.dial CSV,avoiding: :air @20:59:36.48 CSV.dial CTV @20:59:36.93 CS.reconfigure :pressurize @20:59:36.98 CS.seek 0ml @20:59:56.35 CSV.dial :air @20:59:56.80 CS.reconfigure :fast @20:59:56.86 CS.seek 10ml @21:00:00.50 PBV.dial between PSV and waste1 @21:00:00.87 PSV.dial :air @21:00:01.34 PS.reconfigure :fast @21:00:01.40 PS.seek 2.5ml @21:00:21.31 CSV.dial between air and CTV @21:00:21.64 CS.seek 7.33ml @21:00:25.13 PSV.dial :mixloop @21:00:25.60 delay 2 seconds @21:00:27.62 PSV.dial :air @21:00:28.09 delay 2 seconds @21:00:28.57 CTV.dial CSV,avoiding: :air @21:00:28.60 CSV.dial CTV @21:00:28.99 CS.reconfigure :pressurize @21:00:29.04 CS.seek 0ml @21:00:30.10 PSV.dial :mixloop @21:00:30.57 delay 2 seconds @21:00:32.59 PSV.dial :air @21:00:33.07 PS.seek 0ml @21:00:48.41 CSV.dial :air @21:00:49.30 CS.reconfigure :fast @21:00:49.36 CS.seek 10ml @21:00:56.20 washCycleDA #1 of 2 @21:00:56.21 PSR.select :air @21:00:56.23 PSV.dial PSR @21:00:57.54 Pulling 0.5ml of PSR.wash @21:00:57.55 PSR.select :wash @21:00:57.67 PS.reconfigure :default @21:00:57.72 PS.seek 0.5ml @21:01:03.73 delay 5 seconds @21:01:08.75 PSR.select :air @21:01:08.87 PS.seek 2ml @21:01:13.85 CSV.dial between air and CTV @21:01:14.17 CS.seek 7.33ml @21:01:21.07 CTV.dial CSV,avoiding: :air @21:01:21.10 CSV.dial CTV @21:01:21.49 CS.reconfigure :pressurize @21:01:21.54 CS.seek 0ml @21:01:25.04 PTV.dial :puck @21:01:25.77 PSV.dial PTV @21:01:26.63 PBV.dial :waste1 @21:01:27.07 PS.seek 1.5ml @21:01:32.95 delay 3 seconds @21:01:35.96 PBV.dial between PSV and waste1 @21:01:36.35 PS.seek 0ml @21:01:40.81 CSV.dial :air @21:01:41.27 CS.reconfigure :default @21:01:41.32 CS.hold @21:01:41.44 ShallowSampler::Error in MAIN -- Puck.from 2, :wcr reached only 3.3 of 35psi after pumping in 40ml of gas @21:01:41.46 Waiting to Resume from Checkpoint "Pumping wcr puck from tube 2 up to 35psi..." @21:01:41.48 BadNews.email "Puck.from 2, :wcr reached only 3.3 of 35psi after pumping in 40ml of gas",Subject: "ShallowSampler::Error in MAIN" @21:01:45.85 upsync @21:01:52.67 delay 2:00 @21:03:52.70 recoverPSRreagent08 @21:03:52.71 PTV.dial :puck @21:03:52.79 PBV.dial :waste1 @21:03:53.15 delay 30 seconds @21:04:23.17 PBV.dial between PSV and waste1 @21:04:23.55 PSR.select :air @21:04:23.57 PSV.dial PSR @21:04:24.43 PS.reconfigure :fast @21:04:24.49 PS.seek 2.5ml @21:04:48.44 PSV.dial PTV @21:04:49.79 delay 5 seconds @21:04:54.81 PSV.dial PSR @21:04:55.54 delay 5 seconds @21:05:00.56 PSV.dial PTV @21:05:01.30 delay 5 seconds @21:05:06.32 PSV.dial :air @21:05:07.20 PS.seek 0ml @21:05:30.29 washCycleDA #2 of 2 @21:05:30.30 PSR.select :air @21:05:30.31 PSV.dial PSR @21:05:31.63 Pulling 0.5ml of PSR.wash @21:05:31.64 PSR.select :wash @21:05:31.76 PS.reconfigure :default @21:05:31.81 PS.seek 0.5ml @21:05:37.75 delay 5 seconds @21:05:42.77 PSR.select :air @21:05:42.90 PS.seek 2ml @21:05:59.03 PTV.dial :puck @21:05:59.11 PSV.dial PTV @21:05:59.86 PBV.dial :waste1 @21:06:00.30 PS.seek 1.5ml @21:06:06.31 delay 3 seconds @21:06:09.33 PBV.dial between PSV and waste1 @21:06:09.70 PS.seek 0ml @21:06:25.75 delay 2:00 @21:08:25.78 recoverPSRreagent08 @21:08:25.79 PTV.dial :puck @21:08:25.86 PBV.dial :waste1 @21:08:26.23 delay 30 seconds @21:08:56.26 PBV.dial between PSV and waste1 @21:08:56.64 PSR.select :air @21:08:56.66 PSV.dial PSR @21:08:57.41 PS.reconfigure :fast @21:08:57.46 PS.seek 2.5ml @21:09:21.15 PSV.dial PTV @21:09:21.91 delay 5 seconds @21:09:26.93 PSV.dial PSR @21:09:27.71 delay 5 seconds @21:09:32.73 PSV.dial PTV @21:09:33.48 delay 5 seconds @21:09:38.51 PSV.dial :air @21:09:39.25 PS.seek 0ml @21:10:02.56 getPMLReagent08 @21:10:02.57 PRV.dial :air @21:10:03.03 delay 5 seconds @21:10:08.05 PTV.dial :PRVmixing @21:10:09.36 delay 5 seconds @21:10:14.38 PRV.dial :ab2 @21:10:14.81 PTV.dial :PRVmixing @21:10:14.89 PSV.dial :mixloop @21:10:15.36 PS.reconfigure :default @21:10:15.41 PS.seek 0.3ml @21:10:19.31 delay 2 seconds @21:10:21.33 PRV.dial :air @21:10:21.85 PS.seek 0.55ml @21:10:25.32 addPMLReagent08 @21:10:25.33 PSV.dial :air @21:10:25.80 PS.seek 2ml @21:10:41.40 PSV.dial :PML @21:10:41.87 PTV.dial :mixing @21:10:42.62 PBV.dial :waste1 @21:10:43.51 PS.seek 1.85ml @21:10:45.84 PBV.dial between PSV and waste1 @21:10:46.21 PS.seek 0ml @21:11:05.93 delay 2 seconds @21:11:07.95 delay 8:00 @21:19:07.98 recoverPRVreagent08 @21:19:07.99 PTV.dial :mixing @21:19:08.17 PBV.dial :waste1 @21:19:08.55 delay 30 seconds @21:19:38.58 PBV.dial between PSV and waste1 @21:19:38.95 PSV.dial :air @21:19:39.43 PS.reconfigure :fast @21:19:39.48 PS.seek 2.5ml @21:20:03.20 PSV.dial :mixloop @21:20:03.67 delay 2 seconds @21:20:05.69 PSV.dial :air @21:20:06.27 delay 2 seconds @21:20:08.28 PSV.dial :mixloop @21:20:08.74 delay 2 seconds @21:20:10.75 PSV.dial :air @21:20:11.22 PS.seek 0ml @21:20:34.46 washCycleDA #1 of 3 @21:20:34.47 PSR.select :air @21:20:34.48 PSV.dial PSR @21:20:35.80 Pulling 0.5ml of PSR.wash @21:20:35.81 PSR.select :wash @21:20:35.93 PS.reconfigure :default @21:20:35.98 PS.seek 0.5ml @21:20:42.14 delay 5 seconds @21:20:47.16 PSR.select :air @21:20:47.28 PS.seek 2ml @21:21:03.42 PTV.dial :puck @21:21:04.17 PSV.dial PTV @21:21:04.90 PBV.dial :waste1 @21:21:05.34 PS.seek 1.5ml @21:21:11.33 delay 3 seconds @21:21:14.35 PBV.dial between PSV and waste1 @21:21:14.71 PS.seek 0ml @21:21:30.89 delay 2:00 @21:23:30.92 recoverPSRreagent08 @21:23:30.93 PTV.dial :puck @21:23:31.11 PBV.dial :waste1 @21:23:31.48 delay 30 seconds @21:24:01.51 PBV.dial between PSV and waste1 @21:24:01.89 PSR.select :air @21:24:01.90 PSV.dial PSR @21:24:02.66 PS.reconfigure :fast @21:24:02.71 PS.seek 2.5ml @21:24:26.39 PSV.dial PTV @21:24:27.14 delay 5 seconds @21:24:32.16 PSV.dial PSR @21:24:32.90 delay 5 seconds @21:24:37.92 PSV.dial PTV @21:24:38.67 delay 5 seconds @21:24:43.69 PSV.dial :air @21:24:44.56 PS.seek 0ml @21:25:07.70 washCycleDA #2 of 3 @21:25:07.71 PSR.select :air @21:25:07.73 PSV.dial PSR @21:25:09.04 Pulling 0.5ml of PSR.wash @21:25:09.05 PSR.select :wash @21:25:09.17 PS.reconfigure :default @21:25:09.22 PS.seek 0.5ml @21:25:15.54 delay 5 seconds @21:25:20.56 PSR.select :air @21:25:20.68 PS.seek 2ml @21:25:37.01 PTV.dial :puck @21:25:37.09 PSV.dial PTV @21:25:37.84 PBV.dial :waste1 @21:25:38.27 PS.seek 1.5ml @21:25:44.20 delay 3 seconds @21:25:47.22 PBV.dial between PSV and waste1 @21:25:47.59 PS.seek 0ml @21:26:03.98 delay 2:00 @21:28:04.01 recoverPSRreagent08 @21:28:04.02 PTV.dial :puck @21:28:04.08 PBV.dial :waste1 @21:28:04.46 delay 30 seconds @21:28:34.49 PBV.dial between PSV and waste1 @21:28:34.86 PSR.select :air @21:28:34.88 PSV.dial PSR @21:28:35.64 PS.reconfigure :fast @21:28:35.69 PS.seek 2.5ml @21:28:59.22 PSV.dial PTV @21:28:59.98 delay 5 seconds @21:29:05.00 PSV.dial PSR @21:29:05.74 delay 5 seconds @21:29:10.76 PSV.dial PTV @21:29:11.51 delay 5 seconds @21:29:16.53 PSV.dial :air @21:29:17.40 PS.seek 0ml @21:29:40.60 washCycleDA #3 of 3 @21:29:40.61 PSR.select :air @21:29:40.63 PSV.dial PSR @21:29:41.94 Pulling 0.5ml of PSR.wash @21:29:41.95 PSR.select :wash @21:29:42.07 PS.reconfigure :default @21:29:42.12 PS.seek 0.5ml @21:29:48.10 delay 5 seconds @21:29:53.12 PSR.select :air @21:29:53.24 PS.seek 2ml @21:30:09.41 PTV.dial :puck @21:30:09.49 PSV.dial PTV @21:30:10.24 PBV.dial :waste1 @21:30:10.67 PS.seek 1.5ml @21:30:16.97 delay 3 seconds @21:30:19.99 PBV.dial between PSV and waste1 @21:30:20.35 PS.seek 0ml @21:30:36.47 delay 2:00 @21:32:36.50 recoverPSRreagent08 @21:32:36.51 PTV.dial :puck @21:32:36.58 PBV.dial :waste1 @21:32:36.96 delay 30 seconds @21:33:06.98 PBV.dial between PSV and waste1 @21:33:07.36 PSR.select :air @21:33:07.38 PSV.dial PSR @21:33:08.13 PS.reconfigure :fast @21:33:08.18 PS.seek 2.5ml @21:33:31.90 PSV.dial PTV @21:33:32.66 delay 5 seconds @21:33:37.68 PSV.dial PSR @21:33:38.42 delay 5 seconds @21:33:43.44 PSV.dial PTV @21:33:44.27 delay 5 seconds @21:33:49.29 PSV.dial :air @21:33:50.04 PS.seek 0ml @21:34:13.22 Gate.power :camera,:ON @21:34:13.23 Mixing DA Substrates @21:34:13.24 PSR.select :air @21:34:13.26 PSV.dial PSR @21:34:14.64 Pulling 0.25ml of PSR.sub1 @21:34:14.67 PSR.select :sub1 @21:34:14.88 PS.reconfigure :default @21:34:14.99 PS.seek 0.25ml @21:34:18.38 delay 5 seconds @21:34:23.40 Pulling 0.25ml of PSR.sub2 @21:34:23.42 PSR.select :sub2 @21:34:23.54 PS.seek 0.5ml @21:34:26.97 delay 5 seconds @21:34:31.99 PSR.select :air @21:34:32.10 PS.seek 2ml @21:34:48.31 PTV.dial :puck @21:34:48.39 PSV.dial PTV @21:34:49.14 PBV.dial :waste1 @21:34:49.58 PS.seek 1.5ml @21:34:55.59 delay 3 seconds @21:34:58.61 PBV.dial between PSV and waste1 @21:34:58.99 PS.seek 0ml @21:35:15.18 delay 10 seconds @21:35:25.21 recoverPSRreagent08 @21:35:25.22 PTV.dial :puck @21:35:25.29 PBV.dial :waste1 @21:35:25.67 delay 30 seconds @21:35:55.70 PBV.dial between PSV and waste1 @21:35:56.07 PSR.select :air @21:35:56.09 PSV.dial PSR @21:35:56.86 PS.reconfigure :fast @21:35:56.91 PS.seek 2.5ml @21:36:21.12 PSV.dial PTV @21:36:21.89 delay 5 seconds @21:36:26.91 PSV.dial PSR @21:36:27.67 delay 5 seconds @21:36:32.69 PSV.dial PTV @21:36:33.45 delay 5 seconds @21:36:38.47 PSV.dial :air @21:36:39.25 PS.seek 0ml @21:37:03.00 Additional air clear @21:37:03.01 PSR.select :air @21:37:03.02 PSV.dial PSR @21:37:04.37 Pulling 2ml of PSR.air @21:37:04.38 PSR.select :air @21:37:04.40 PS.reconfigure :default @21:37:04.45 PS.seek 2ml @21:37:25.90 delay 5 seconds @21:37:30.92 PTV.dial :puck @21:37:30.99 PSV.dial PTV @21:37:31.74 PBV.dial :waste1 @21:37:32.18 PS.seek 1.5ml @21:37:38.21 delay 3 seconds @21:37:41.23 PBV.dial between PSV and waste1 @21:37:41.60 delay 1 second @21:37:42.62 PSV.dial :waste1 @21:37:43.69 PS.seek 0ml @21:37:59.89 Processing Puck Heater at 27.9C before imaging @21:37:59.90 PH.coast @21:38:00.01 Imaging da puck in Processing Clamp while storing it to tube 1 @21:38:00.67 SC.reconfigure :tight @21:38:00.72 SC.seek 1 @21:38:04.37 SC.configure :limp @21:38:04.59 Elbow.reconfigure :tight @21:38:04.64 Elbow.seek 1 @21:38:06.41 Forearm.reconfigure :tight @21:38:06.47 Forearm.seek 1 + 450 counts @21:38:07.59 SE.reconfigure :pick @21:38:07.65 SE.seek :beamBreak @21:38:10.73 SE.configure :default @21:38:10.89 Elbow.reconfigure :limp @21:38:10.95 Forearm.reconfigure :loose @21:38:10.99 Forearm.seek 1 @21:38:11.23 Elbow.hold @21:38:11.30 Elbow.reconfigure :tight @21:38:11.40 SE.seek 1.15 Pucks below Top Plate @21:38:11.82 Forearm.force -20 @21:38:11.86 Forearm.seek 1 + 307 counts @21:38:12.16 Forearm.reconfigure :tight @21:38:12.21 Forearm.seek 1 + 450 counts @21:38:12.39 SE.seek 2.15 Pucks below Top Plate @21:38:12.90 Forearm.reconfigure :loose @21:38:12.95 Forearm.seek :retract @21:38:13.88 Elbow.seek PC @21:38:16.37 Elbow.reconfigure :loose @21:38:16.47 Forearm.seek PC @21:38:17.43 Hand.close @21:38:18.09 PC.seek :open @21:38:22.85 Forearm.seek :retract @21:38:23.83 Elbow.reconfigure :tight @21:38:23.88 Elbow.seek 1 @21:38:26.96 Forearm.reconfigure :tight @21:38:27.02 Forearm.seek 1 + 450 counts @21:38:27.99 Hand.open @21:38:28.65 SE.seek 1.8 Pucks below Top Plate @21:38:28.93 SE.reconfigure :seal @21:38:28.98 SE.seek 0.84 Pucks below Top Plate @21:38:29.57 Lights, camera, ACTION! @21:38:29.61 PuckCamera: snap -bin=2,2 -auto /var/log/esp/da24jan1821h30ml.tif @21:38:37.37 SX HX9/16 (USB:04): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 3.506 seconds /var/log/esp/da24jan1821h30ml.tif: TIFF Upload Complete @21:38:37.40 PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da24jan1821h30ml15s.tif @21:38:49.04 Waiting >20 seconds for da to relinquish FlushPuck @21:38:53.12 SX HX9/16 (USB:04): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15 seconds /var/log/esp/midres/da24jan1821h30ml15s.tif: TIFF Upload Complete @21:38:53.15 PuckCamera: snap -bin=2,2 20.0 /var/log/esp/midres/da24jan1821h30ml20s.tif @21:39:13.82 SX HX9/16 (USB:04): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 20 seconds /var/log/esp/midres/da24jan1821h30ml20s.tif: TIFF Upload Complete @21:39:13.84 Gate.power :camera,:OFF @21:39:13.86 DAfans.email "3.506 sec auto-exposure",Subject: "da24jan1821h30ml images!" @21:39:14.30 Elbow.seek 1 @21:39:14.54 Forearm.seek 1 + 450 counts @21:39:14.64 SE.reconfigure :gently @21:39:14.71 SE.seek 1.55 Pucks below Top Plate @21:39:15.38 Elbow.reconfigure :limp @21:39:15.46 Forearm.reconfigure :loose @21:39:15.51 Forearm.seek 1 @21:39:15.78 Elbow.hold @21:39:15.87 Elbow.reconfigure :tight @21:39:15.92 SE.reconfigure :default @21:39:15.97 SE.seek 1.74 Pucks below Top Plate @21:39:16.37 Forearm.force -20 @21:39:16.42 Forearm.seek 1 + 306 counts @21:39:16.83 Forearm.reconfigure :tight @21:39:16.88 Forearm.seek 1 + 450 counts @21:39:17.30 SE.seek :down @21:39:18.59 Forearm.reconfigure :loose @21:39:18.71 Forearm.seek :retract @21:39:20.01 SE.stop @21:39:20.28 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @21:39:21.77 Elbow.seek FlushPuck::Garage @21:39:23.76 Elbow.reconfigure :loose @21:39:23.96 Forearm.seek :garage @21:39:25.03 Hand.close @21:39:26.14 Forearm.seek :retract @21:39:27.19 Elbow.reconfigure :tight @21:39:27.27 Elbow.seek PC @21:39:28.65 Elbow.reconfigure :loose @21:39:28.91 Forearm.reconfigure :tight @21:39:29.01 Forearm.seek PC @21:39:30.62 PC.seek :closed @21:39:34.37 upsync @21:39:35.33 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.65 @21:39:35.40 Hand.open @21:39:36.02 Forearm.reconfigure :loose @21:39:36.07 Forearm.seek :retract @21:39:37.06 PSR.select :air @21:39:37.08 PSV.dial PSR @21:39:37.54 Pulling 0.5ml of PSR.flush @21:39:37.55 PSR.select :flush @21:39:37.67 PS.seek 0.5ml @21:39:43.65 delay 5 seconds @21:39:48.67 PSR.select :air @21:39:48.79 PS.seek 2.5ml @21:40:10.24 delay 1 second @21:40:11.26 PRV.dial :waste2,avoiding: :ab2 @21:40:13.10 PTV.dial :PRVmixing @21:40:14.40 PSV.dial :mixloop @21:40:15.54 PS.seek :empty @21:40:41.86 PSR.select :air @21:40:41.88 PSV.dial PSR @21:40:42.91 Pulling 0.5ml of PSR.flush @21:40:42.92 PSR.select :flush @21:40:43.49 PS.seek 0.5ml @21:40:49.46 delay 5 seconds @21:40:54.48 PSR.select :air @21:40:54.60 PS.seek 2.5ml @21:41:15.80 delay 1 second @21:41:16.82 PBV.dial :waste1 @21:41:17.18 PTV.dial :mixing @21:41:17.93 PSV.dial :mixloop @21:41:18.98 PS.seek :empty @21:41:45.36 PSV.dial PSR @21:41:46.40 PSR.select :flush @21:41:46.52 PS.seek 1ml @21:41:57.61 PSR.select :air @21:41:57.73 PS.seek 2.5ml @21:42:13.83 PSV.dial PTV @21:42:14.71 PTV.dial :puck @21:42:15.44 PBV.dial :waste1 @21:42:15.53 PS.seek 0ml @21:42:41.86 PSV.dial PSR @21:42:42.60 PSR.select :flush @21:42:42.72 PS.seek 0.5ml @21:42:48.75 PSR.select :air @21:42:48.92 PS.seek 2.5ml @21:43:10.49 PSV.dial PTV @21:43:11.24 PBV.dial PSV @21:43:11.79 PS.seek 0ml @21:43:38.11 PSV.dial PBV @21:43:38.59 PS.seek 2.5ml @21:44:05.11 PSV.dial :waste1 @21:44:05.85 PS.seek 0ml @21:44:32.17 PSV.dial PSR @21:44:32.64 PSR.select :air @21:44:32.67 PS.seek 2.5ml @21:44:59.05 PSV.dial PTV @21:44:59.78 PBV.dial PSV @21:44:59.87 PS.seek 0ml @21:45:26.23 PSV.dial PBV @21:45:26.71 PS.seek 2.5ml @21:45:53.01 PSV.dial :waste1 @21:45:53.76 PS.seek 0ml @21:46:20.14 PSV.dial PSR @21:46:20.72 PSR.select :flush @21:46:20.84 PS.seek 1ml @21:46:31.92 PSR.select :air @21:46:32.04 PS.seek 2.5ml @21:46:48.92 PSV.dial PTV @21:46:49.67 PTV.dial :puck @21:46:49.75 PBV.dial :waste1 @21:46:50.30 PS.seek 0ml @21:47:16.67 PSV.dial PSR @21:47:17.42 PSR.select :flush @21:47:17.54 PS.seek 0.5ml @21:47:23.42 PSR.select :air @21:47:23.54 PS.seek 2.5ml @21:47:44.76 PSV.dial PTV @21:47:45.51 PBV.dial PSV @21:47:46.07 PS.seek 0ml @21:48:12.48 PSV.dial PBV @21:48:12.96 PS.seek 2.5ml @21:48:39.51 PSV.dial :waste1 @21:48:40.25 PS.seek 0ml @21:49:06.63 PSV.dial PSR @21:49:07.09 PSR.select :air @21:49:07.11 PS.seek 2.5ml @21:49:33.51 PSV.dial PTV @21:49:34.24 PBV.dial PSV @21:49:34.33 PS.seek 0ml @21:50:00.66 PSV.dial PBV @21:50:01.14 PS.seek 2.5ml @21:50:27.54 PSV.dial :waste1 @21:50:28.28 PS.seek 0ml @21:50:54.64 Garaging FlushPuck in Processing Clamp @21:50:54.71 Elbow.reconfigure :tight @21:50:54.76 Elbow.seek PC @21:50:54.88 Elbow.reconfigure :loose @21:50:54.98 Forearm.seek PC @21:50:55.94 Hand.close @21:50:56.58 PC.seek :open @21:51:01.31 Forearm.seek :retract @21:51:02.27 Elbow.reconfigure :tight @21:51:02.32 Elbow.seek :garage @21:51:03.68 Elbow.reconfigure :loose @21:51:03.78 Forearm.reconfigure :tight @21:51:03.83 Forearm.seek :garage @21:51:04.86 Hand.open @21:51:05.95 Forearm.reconfigure :loose @21:51:06.00 Forearm.seek :retract + ESPwhich DA_WCR_011024.rb + fn=DA_WCR_011024.rb + IFS=: + set /home/esp/esp2/mission . + fullPath=/home/esp/esp2/mission/DA_WCR_011024.rb + [ -f /home/esp/esp2/mission/DA_WCR_011024.rb ] + realpath /home/esp/esp2/mission/DA_WCR_011024.rb + return 0 + scriptPath=/home/esp/esp2local/mission/DA_WCR_011024.rb + exec ruby /home/esp/esp2/lib/esp.rb /home/esp/esp2local/mission/DA_WCR_011024.rb @13:56:23.37UTC19-Jan-24
ESP2 v3.42 I2C Gateway v3.20 for core bus @13:56:26.12 Configuration for short puck jake 1/11/21 by brent@mbari.org /home/esp/esp2/type/shallow/jake/configure.rb @13:56:29.94 Polling Can every 10:00 @13:56:29.99 Loading /home/esp/esp2local/mission/DA_WCR_011024.rb mission @13:56:30.05 GoodNews.email "Starting DA_WCR_011024 w/pucks in tube 2\n{}\nCTD data unavailable\nCan@13:56:29, 25% flow, 22.5C, 12% humidity, 14.8psia, 13.822V, 0.260A, 64.198Ah, 3.60W",Subject: "DA_WCR_011024 begun" @13:56:30.08
Preparing to sleep until + 00:00:05 @13:56:30.13 Asleep til + 00:00:05 @13:56:30.15 Continuing at 13:56 @13:56:30.44 Gate.power :core,:OFF @13:56:30.45 Gate.power :raw,:OFF @13:56:30.46 Shutdown @13:56:33.00 GoodNews.email "Then, in tube 2: initialPurge; da [50, 50] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_011024.rb:14\n{1 => 0,\n 2 => 4,\n 3 => 0,\n 4 => 0,\n 5 => 0,\n 6 => 0,\n 7 => 0}\nCTD data unavailable\nCan@13:56:30, 25% flow, 22.4C, 12% humidity, 14.8psia, 13.827V, 0.266A, 64.198Ah, 3.68W",Subject: "Asleep until 13:56" @13:56:34.45 upsync @13:56:35.04
Powering core bus @13:56:36.90 upsync @13:56:40.66
Gate.power :raw,:ON @13:56:42.05 CS.configure :default @13:56:42.09 PS.configure :default @13:56:42.14 SS.configure :default @13:56:42.19 CC.configure :default @13:56:42.24 PC.configure :default @13:56:42.31 Forearm.configure :loose @13:56:42.36 Elbow.configure :loose @13:56:42.43 SC.configure :loose @13:56:42.47 SE.configure :default @13:56:42.52 CH.configure :default @13:56:42.56 PH.configure :default @13:56:42.59 All dwarf controllers running firmware version 3.85 @13:56:42.70 Valve.reset @13:56:43.04 PSV.dial :air @13:56:43.06 Intake.select :close @13:56:43.11 Exhaust.select :close @13:56:43.15 CTV.dial :air @13:56:43.22
Forearm.home.seek :retract @13:56:43.92 Hand.close @13:56:43.95
Elbow.reconfigure :loose @13:56:44.04 Elbow.jog 1500 @13:56:44.14 PS.home.seek 0ml @13:56:44.46 CSV.dial :waste1 @13:56:44.60
Elbow.reconfigure :loose @13:56:44.65 Elbow.home.seek :clear @13:56:45.18 SSV.dial :spit,avoiding: :exhaust @13:56:46.03 CS.reconfigure :fast @13:56:46.06 SS.home.seek 0ml @13:56:46.14 CS.home.seek 0ml @13:56:47.87
SE.home.seek :down @13:56:48.47 SC.reconfigure :tight @13:56:48.53 SC.home.seek :initial @13:56:52.34 PS.stop @13:57:13.66
SC.configure :limp @13:57:13.71 Forearm.seek :retract @13:57:13.78 Elbow.seek :hammer @13:57:14.87 Forearm.reconfigure :tight @13:57:14.92 Forearm.seek retracted @13:57:15.18 PC.home.seek :open @13:57:15.22
CC.home.seek :open @13:57:18.67 Phase [initialPurge; da [50, 50] #da and wsr are run simultanelously] @13:57:18.70 Notice.email "At 13:57:18 began in tube 2: initialPurge; da [50, 50] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_011024.rb:14\n{1 => 0,\n 2 => 4,\n 3 => 0,\n 4 => 0,\n 5 => 0,\n 6 => 0,\n 7 => 0}\nCTD data unavailable\nCan@13:57:18, 25% flow, 22.6C, 12% humidity, 14.8psia, 13.827V, 0.236A, 64.203Ah, 3.26W",Subject: "Started DA_WCR_011024 phase initialPurge; da [50, 50] #da and wsr are run simultanelously" @13:57:18.72
Starting initialPurge @13:57:18.75 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @13:57:18.81 Hand.open @13:57:19.60 Elbow.reconfigure :tight @13:57:19.65 Elbow.seek FlushPuck::Garage @13:57:21.57 Elbow.reconfigure :loose @13:57:21.80 Forearm.reconfigure :loose @13:57:21.88 Forearm.seek :garage @13:57:22.89 Hand.close @13:57:22.91 upsync @13:57:23.60
Forearm.seek :retract @13:57:24.62 Elbow.reconfigure :tight @13:57:24.67 Elbow.seek CC @13:57:27.56 Elbow.reconfigure :loose @13:57:27.67 Forearm.reconfigure :tight @13:57:27.72 Forearm.seek CC @13:57:28.94 CC.seek :closed @13:57:33.50 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.35 @13:57:33.58 Hand.open @13:57:34.29 Forearm.reconfigure :loose @13:57:34.33 Forearm.seek :retract @13:57:35.34 Flushing the Collection Valve::Manifold @13:57:35.47 CSV.dial CSR @13:57:36.76 Pulling 5ml of CSR.flush CSR.select :flush @13:57:36.88 CS.reconfigure :default @13:57:36.93 CS.seek 5ml @13:57:50.49 delay 5 seconds @13:57:55.51 CSR.select :air @13:57:56.11 CS.reconfigure :fast @13:57:56.15 CS.seek 7.5ml @13:58:02.89 CSV.dial :waste @13:58:04.16 CS.reconfigure :default @13:58:04.21 CS.seek 0ml @13:58:24.21 Purging external Intake @13:58:24.34 CSV.dial :flush @13:58:24.79 CS.reconfigure :fast @13:58:24.84 CS.seek 10ml @13:58:49.39 delay 5 seconds @13:58:54.41 CSV.dial CBV @13:58:54.45
CBV.dial CSV @13:58:55.24 CTV.dial :intake @13:58:55.99 Intake.select :open @13:58:56.10 CS.seek 0ml @13:59:19.64 Intake.select :close @13:59:19.75 CTV.dial :air @13:59:20.52 Flushing upper manifold @13:59:20.64 CSV.dial :flush @13:59:21.37 CS.seek 5ml @13:59:34.20 delay 5 seconds @13:59:39.22 CSV.dial :air @13:59:39.72 CS.seek 7ml @13:59:45.24 CSV.dial CBV @13:59:45.26
CBV.dial CSV @13:59:46.30 CTV.dial :air @13:59:46.38 CS.seek 0ml @14:00:03.05 delay 7 seconds @14:00:10.08 CS.seek 3ml @14:00:18.11 CSV.dial :waste @14:00:18.57 CS.seek 0ml @14:00:26.41 CSV.dial :flush @14:00:26.85 CS.seek 3ml @14:00:34.98 delay 5 seconds @14:00:40.00 CSV.dial :air @14:00:40.50 CS.seek 5ml @14:00:46.04 CSV.dial :air @14:00:46.22 CS.seek 0ml @14:00:58.33 delay 7 seconds @14:01:05.36 CS.seek 3ml @14:01:13.39 CSV.dial :waste @14:01:14.12 CS.seek 0ml @14:01:21.64 Garaging FlushPuck in Collection Clamp @14:01:21.71 Elbow.reconfigure :tight @14:01:21.76 Elbow.seek CC @14:01:22.06 Elbow.reconfigure :loose @14:01:22.16 Forearm.seek CC @14:01:23.20 Hand.close @14:01:23.85 CC.seek :open @14:01:28.89 Forearm.seek :retract @14:01:29.94 Elbow.reconfigure :tight @14:01:29.99 Elbow.seek :garage @14:01:32.87 Elbow.reconfigure :loose @14:01:32.97 Forearm.reconfigure :tight @14:01:33.02 Forearm.seek :garage @14:01:34.09 Hand.open @14:01:34.78 Forearm.reconfigure :loose @14:01:34.83 Forearm.seek :retract @14:01:35.83 da sampling at most 50ml, wcr at most 50ml @14:01:35.87 DAfans.email "Started da in tube 2 at 14:01:35UTC19-Jan-24\n{1 => 0,\n 2 => 4,\n 3 => 0,\n 4 => 0,\n 5 => 0,\n 6 => 0,\n 7 => 0}\nCTD data unavailable\nCan@13:57:18, 25% flow, 22.6C, 12% humidity, 14.8psia, 13.827V, 0.236A, 64.203Ah, 3.26W",Subject: "da sampling at most 50ml, wcr at most 50ml" @14:01:35.91
Loading sh1 puck from tube 2 into Collection Clamp @14:01:36.15 SC.reconfigure :tight @14:01:36.20 SC.seek 2 @14:01:41.02 upsync @14:01:42.92
SC.configure :limp @14:01:43.15 Elbow.reconfigure :tight @14:01:43.21 Elbow.seek 2 @14:01:45.21 Forearm.reconfigure :tight @14:01:45.26 Forearm.seek 2 + 450 counts @14:01:46.29 SE.reconfigure :pick @14:01:46.35 SE.seek :beamBreak @14:01:49.27 SE.configure :default @14:01:49.44 Elbow.seek 2 @14:01:49.74 Forearm.seek 2 + 300 counts @14:01:50.03 Forearm.reconfigure :loose @14:01:50.08 Hand.close @14:01:50.74 SE.seek :down @14:01:50.95
Forearm.seek :retract @14:01:51.94 Elbow.seek CC @14:01:53.37 SE.stop @14:01:53.58
Elbow.reconfigure :loose @14:01:53.68 Forearm.reconfigure :tight @14:01:53.73 Forearm.seek CC @14:01:55.01 CC.seek :closed @14:01:59.52 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.28 @14:01:59.53 Hand.open @14:02:00.16 Forearm.reconfigure :loose @14:02:00.21 Forearm.seek :retract @14:02:01.22 collectSample 50ml w/bubble point of 24psi @14:02:01.24 Priming 7ml @14:02:01.25 Intake.select :close @14:02:01.26 Exhaust.select :close @14:02:01.27 SS.hold @14:02:01.48 Calibrating pressure and temperature sensors @14:02:01.51 CTV.dial :air @14:02:01.59 SSV.dial :spit,avoiding: :exhaust @14:02:01.67 SS.reconfigure :fast @14:02:01.72 SS.seek 5ml @14:02:04.48 SSV.dial CBV @14:02:04.51
CBV.dial SSV @14:02:05.84 delay 3.8 seconds @14:02:09.77 Sensor limits: Intake max 115.7psia; Outlet max 109.7psia @14:02:09.78 SSV.dial :spit @14:02:11.10 SS.seek 0ml @14:02:13.90 SSV.dial CBV @14:02:15.22 CTV.dial :intake @14:02:16.00 SSV.dial CBV @14:02:16.03
CBV.dial SSV @14:02:16.17 Allowing 40s for 7.0ml of remaining 7.0ml @14:02:16.18 Intake.select :open @14:02:18.35 Intake @15.0psia, vacuum from 9.5 to 12.5psi @14:02:18.36 SS.reconfigure :inhaling 2.20ml/s @14:02:18.41 SS.seek 7ml @14:02:24.91 Stroke completed -- waiting for pressure to equalize ... @14:02:24.92 SS.reconfigure :exhale @14:02:24.97 SS.hold @14:02:29.29 Intake.select :close @14:02:29.44 SSV.dial :exhaust @14:02:30.20 Exhaust.select :open @14:02:30.31 SS.seek 0ml @14:02:34.63 Exhaust.select :close @14:02:34.75 Sampling 50.0ml @14:02:34.76 Intake.select :close @14:02:34.77 Exhaust.select :close @14:02:34.78 SS.reconfigure :default @14:02:34.82 SS.hold @14:02:35.03 CTV.dial :intake @14:02:35.29 SSV.dial CBV @14:02:35.33
CBV.dial SSV @14:02:36.07 Allowing 130s for 25.0ml of remaining 50.0ml @14:02:36.08 Intake.select :open @14:02:38.25 Intake @15.5psia, vacuum from 10.0 to 13.0psi @14:02:38.27 SS.reconfigure :inhaling 2.20ml/s @14:02:38.31 SS.seek 25ml @14:02:48.55 Vacuum too high -- waiting up to 40 seconds @14:02:48.56 SS.reconfigure :exhale @14:02:48.61 SS.hold @14:02:49.32 Intake @12.6psia, vacuum from 7.1 to 10.1psi @14:02:49.34 SS.reconfigure :inhaling 1.76ml/s @14:02:49.39 SS.seek 25ml @14:02:54.74 Vacuum too high -- waiting up to 40 seconds @14:02:54.75 SS.reconfigure :exhale @14:02:54.80 SS.hold @14:02:59.75 Intake @14.5psia, vacuum from 9.0 to 12.0psi @14:02:59.77 SS.reconfigure :inhaling 1.41ml/s @14:02:59.81 SS.seek 25ml @14:03:00.30 Stroke completed -- waiting for pressure to equalize ... @14:03:00.31 SS.reconfigure :exhale @14:03:00.36 SS.hold @14:03:01.09 Intake.select :close @14:03:01.25 SSV.dial :exhaust @14:03:02.00 Exhaust.select :open @14:03:02.11 SS.seek 0ml @14:03:10.90 Exhaust.select :close @14:03:11.01 SSV.dial CBV @14:03:11.04
CBV.dial SSV @14:03:11.79 Allowing 130s for 25.0ml of remaining 25.0ml @14:03:11.80 Intake.select :open @14:03:13.97 Intake @15.6psia, vacuum from 10.0 to 13.0psi @14:03:13.99 SS.reconfigure :inhaling 1.41ml/s @14:03:14.03 SS.seek 25ml @14:03:30.42 Vacuum too high -- waiting up to 40 seconds @14:03:30.43 SS.reconfigure :exhale @14:03:30.49 SS.hold @14:03:33.14 Intake @13.7psia, vacuum from 8.2 to 11.2psi @14:03:33.16 SS.reconfigure :inhaling 1.13ml/s @14:03:33.20 SS.seek 25ml @14:03:39.41 Stroke completed -- waiting for pressure to equalize ... @14:03:39.42 SS.reconfigure :exhale @14:03:39.47 SS.hold @14:03:45.00 Intake.select :close @14:03:45.15 SSV.dial :exhaust @14:03:45.88 Exhaust.select :open @14:03:46.00 SS.seek 0ml @14:03:55.30 Exhaust.select :close @14:03:55.41 Sampled 50.0ml @14:03:55.42 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @14:03:55.43 Intake.select :close @14:03:55.50 CSV.dial :waste @14:03:55.67 SSV.dial CBV,avoiding: :exhaust @14:03:55.70 CS.seek 0ml @14:03:55.73
CBV.dial SSV @14:03:55.88 CSV.dial :air @14:03:56.63 CS.seek 10ml @14:03:57.68
CTV.dial :air @14:03:58.42 delay 15 seconds @14:04:13.44 SSV.dial :spit,avoiding: :exhaust @14:04:14.76 CTV.dial CSV,avoiding: :intake @14:04:14.80
SS.seek 0ml @14:04:14.93 SSV.dial CBV,avoiding: :exhaust @14:04:20.98 SSV.dial between CBV and plug @14:04:21.47 CBV.dial between SSV and waste2 @14:04:21.86 CTV.dial CSV,avoiding: :air @14:04:21.89
CSV.dial CTV @14:04:22.41 CS.reconfigure :pressurize @14:04:22.46 CS.seek 0ml @14:04:40.73 BubblePoint: Intake @24.1psia, Outlet @0.5psia @14:04:40.74 CBV.dial SSV @14:04:41.09 SS.reconfigure :default @14:04:41.14 SS.seek 10ml @14:04:46.84 delay 15 seconds @14:05:01.97 Evacuated: Intake @23.9psia, Outlet @24.2psia @14:05:01.98 SSV.dial :spit,avoiding: :exhaust @14:05:03.01 SS.reconfigure :exhale @14:05:03.06 SS.seek 0ml @14:05:08.13 SS.reconfigure :fast @14:05:08.17 SS.seek 5ml @14:05:11.01 SSV.dial CBV,avoiding: :exhaust @14:05:11.04
CBV.dial SSV @14:05:12.39 CTV.dial :air,avoiding: :intake @14:05:13.67 CSV.dial CTV @14:05:13.71
SS.seek 10ml @14:05:14.92 CSV.dial :air @14:05:15.36 CS.reconfigure :fast @14:05:15.41 CS.seek 0ml @14:05:16.37
SSV.dial :spit,avoiding: :exhaust @14:05:17.70 SS.reconfigure :exhale @14:05:17.75 SS.seek 0ml @14:05:23.03 SS.reconfigure :fast @14:05:23.08 SS.seek 5ml @14:05:23.16 CS.stop @14:05:25.92 SSV.dial CBV,avoiding: :exhaust @14:05:25.95
CBV.dial SSV @14:05:28.28 SSV.dial :spit,avoiding: :exhaust @14:05:29.61 SS.reconfigure :default @14:05:29.65 SS.seek 0ml @14:05:33.37 SS.stop @14:05:33.41 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @14:05:33.42 Intake.select :close @14:05:33.49 CSV.dial :waste @14:05:33.62 SSV.dial CBV,avoiding: :exhaust @14:05:33.65
CBV.dial SSV @14:05:34.26 CS.seek 0ml @14:05:34.33 CSV.dial :air @14:05:34.98
CTV.dial :air @14:05:35.06 CS.seek 10ml @14:05:35.12
delay 15 seconds @14:05:50.14 SSV.dial :spit,avoiding: :exhaust @14:05:51.45 CTV.dial CSV,avoiding: :intake @14:05:51.48
SS.reconfigure :exhale @14:05:51.58 SS.seek 0ml @14:05:52.01 SSV.dial CBV,avoiding: :exhaust @14:05:59.57 SSV.dial between CBV and plug @14:06:00.06 CBV.dial between SSV and waste2 @14:06:00.44 CSV.dial between air and CTV @14:06:00.75 CS.seek 8.2ml @14:06:05.61 CTV.dial CSV,avoiding: :air @14:06:05.64
CSV.dial CTV @14:06:05.98 CS.reconfigure :pressurize @14:06:06.03 CS.seek 0ml @14:06:24.14 BubblePoint: Intake @24.2psia, Outlet @2.3psia @14:06:24.15 CBV.dial SSV @14:06:24.47 SS.reconfigure :default @14:06:24.51 SS.seek 10ml @14:06:30.27 delay 15 seconds @14:06:45.40 Evacuated: Intake @24.0psia, Outlet @24.2psia @14:06:45.41 SSV.dial :spit,avoiding: :exhaust @14:06:46.46 SS.reconfigure :exhale @14:06:46.51 SS.seek 0ml @14:06:51.62 SS.reconfigure :fast @14:06:51.67 SS.seek 5ml @14:06:54.46 SSV.dial CBV,avoiding: :exhaust @14:06:54.49
CBV.dial SSV @14:06:55.85 CTV.dial :air,avoiding: :intake @14:06:57.18 CSV.dial CTV @14:06:57.22
SS.seek 10ml @14:06:58.43 CSV.dial :air @14:06:58.87 CS.reconfigure :fast @14:06:58.92 CS.seek 0ml @14:06:59.74
SSV.dial :spit,avoiding: :exhaust @14:07:01.06 SS.reconfigure :exhale @14:07:01.10 SS.seek 0ml @14:07:02.76 CS.stop @14:07:06.44
SS.reconfigure :fast @14:07:06.48 SS.seek 5ml @14:07:09.34 SSV.dial CBV,avoiding: :exhaust @14:07:09.37
CBV.dial SSV @14:07:12.20 SSV.dial :spit,avoiding: :exhaust @14:07:13.54 SS.reconfigure :default @14:07:13.59 SS.seek 0ml @14:07:16.99 SS.stop @14:07:17.03 Lysing: Adding 2.0ml AqMeOH @14:07:17.04 CBV.dial between CSV and waste1,avoiding: :DBV @14:07:18.49 CTV.dial CSV @14:07:19.79 CSV.dial CSR @14:07:20.69 Pulling 1ml of CSR.AqMeOH @14:07:20.70 CSR.select :AqMeOH @14:07:20.82 CS.reconfigure :default @14:07:20.86 CS.seek 1ml @14:07:24.32 delay 5 seconds @14:07:29.34 CSR.select :air @14:07:29.51 CS.seek 6ml @14:07:43.20 delay 2 seconds @14:07:45.21 CSV.dial :waste2 @14:07:45.67 CS.reconfigure :fast @14:07:45.72 CS.seek 0ml @14:08:00.07 CSV.dial CSR @14:08:00.52 Pulling 2ml of CSR.AqMeOH @14:08:00.53 CSR.select :AqMeOH @14:08:00.65 CS.reconfigure :default @14:08:00.70 CS.seek 2ml @14:08:06.69 delay 5 seconds @14:08:11.71 CSR.select :air @14:08:11.88 CS.seek 5.001ml @14:08:20.60 delay 2 seconds @14:08:22.62 CSV.dial CTV @14:08:23.06 CS.seek 1ml @14:08:34.27 Before lysing Collection Puck Heater at 22.1C/25.2psia @14:08:34.29 Heating Puck.from 2, :sh1 in Collection Clamp to 70C for 10:00 @14:08:34.31 CH.seek 70C,8:00 @14:10:24.97 CH.hold 10:00,70C @14:10:25.16 Begun lysing Collection Puck Heater at 68C/30.7psia @14:20:25.18 Ended lysing Collection Puck Heater at 70C/29.6psia @14:20:25.19 CS.seek 0ml @14:20:28.84 CTV.dial between spare and air,avoiding: :intake @14:20:29.85 CSV.dial :air @14:20:30.30 CS.seek 5ml @14:20:43.96 CSV.dial CTV @14:20:44.42 delay 2 seconds @14:20:46.43 CSV.dial :air @14:20:46.89 delay 2 seconds @14:20:48.90 CSV.dial CTV @14:20:49.36 delay 2 seconds @14:20:51.38 CSV.dial :air @14:20:51.82 delay 2 seconds @14:20:53.84 CS.seek 0ml @14:21:07.52 Puck @58.5psia after final lysis pressurization @14:21:07.53 Recovering Lysate into Collection syringe @14:21:07.54 CSV.dial CBV @14:21:08.54 CBV.dial CSV @14:21:08.91 CS.seek 5ml @14:21:22.46 delay 5 seconds @14:21:27.48 CTV.dial :air @14:21:28.02 CS.seek 7ml @14:21:33.90 delay 5 seconds @14:21:38.92 CSV.dial :air2 @14:21:39.36 CS.seek 10ml @14:21:47.90 CSV.dial between air and CTV,avoiding: CBV @14:21:49.04 Lysing Complete @14:21:49.11 Storing sh1 puck in Collection Clamp to tube 1 @14:21:49.29 SC.reconfigure :tight @14:21:49.34 SC.seek 1 @14:21:53.42 SC.configure :limp @14:21:53.59 Elbow.reconfigure :tight @14:21:53.63 SE.reconfigure :place @14:21:53.70
Elbow.seek CC @14:21:53.79 SE.seek :beamBreak @14:21:54.04
Elbow.reconfigure :loose @14:21:54.14 Forearm.seek CC @14:21:55.19 Hand.close @14:21:55.84 CC.seek :open @14:21:56.64 SE.configure :default @14:21:56.69 SE.seek :place @14:22:00.57
Forearm.seek :retract @14:22:01.58 Elbow.reconfigure :tight @14:22:01.63 Elbow.seek 1 @14:22:03.33 Elbow.reconfigure :limp @14:22:03.38 Forearm.seek 1 @14:22:04.37 Elbow.hold @14:22:04.44 Elbow.reconfigure :tight @14:22:04.49 Hand.open @14:22:05.20 Forearm.force -20 @14:22:05.24 Forearm.seek 1 + 318 counts @14:22:05.54 Forearm.reconfigure :tight @14:22:05.59 Forearm.seek 1 + 450 counts @14:22:05.83 SE.seek :down @14:22:06.97
Forearm.reconfigure :loose @14:22:07.02 Forearm.seek :retract @14:22:08.55 SE.stop @14:22:08.59
Tranferring DA @14:22:08.60 Beginning samplePassC @14:22:08.62 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:22:08.81 Elbow.seek FlushPuck::Garage @14:22:10.60 Elbow.reconfigure :loose @14:22:11.16 Forearm.seek :garage @14:22:12.13 Hand.close @14:22:12.83 Forearm.seek :retract @14:22:13.82 Elbow.reconfigure :tight @14:22:13.87 Elbow.seek CC @14:22:16.80 Elbow.reconfigure :loose @14:22:16.91 Forearm.reconfigure :tight @14:22:16.96 Forearm.seek CC @14:22:18.17 CC.seek :closed @14:22:22.69 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.33 @14:22:22.76 Hand.open @14:22:23.39 Forearm.reconfigure :loose @14:22:23.44 Forearm.seek :retract @14:22:24.39 PSV.dial PSR @14:22:25.76 Pulling 1.5ml of PSR.flush @14:22:25.77 PSR.select :flush @14:22:25.89 PS.seek 1.5ml @14:22:41.97 delay 5 seconds @14:22:46.99 PSV.dial :waste1 @14:22:47.46 PS.seek 0ml @14:23:03.63 PSV.dial PSR @14:23:04.10 PSR.select :air @14:23:04.21 PS.seek 2.5ml @14:23:30.76 PRV.dial CBV,avoiding: :ab2 @14:23:30.80
CBV.dial PRV @14:23:32.52 PSV.dial :mixloop @14:23:33.54 CTV.dial :air,avoiding: :intake @14:23:33.71 PTV.dial :PRVmixing @14:23:35.01 PS.seek 0ml @14:24:01.50 PSV.dial :air @14:24:02.05 PS.seek 2.5ml @14:24:28.34 PSV.dial :mixloop @14:24:28.82 PS.seek 0ml @14:24:55.16 PSV.dial :air @14:24:55.63 PS.seek 2.5ml @14:25:21.97 PSV.dial :mixloop @14:25:22.44 PS.seek 0ml @14:25:48.85 Garaging FlushPuck in Collection Clamp @14:25:48.92 Elbow.reconfigure :tight @14:25:48.97 Elbow.seek CC @14:25:49.20 Elbow.reconfigure :loose @14:25:49.30 Forearm.seek CC @14:25:50.28 Hand.close @14:25:50.95 CC.seek :open @14:25:55.60 Forearm.seek :retract @14:25:56.64 Elbow.reconfigure :tight @14:25:56.69 Elbow.seek :garage @14:25:59.66 Elbow.reconfigure :loose @14:25:59.76 Forearm.reconfigure :tight @14:25:59.81 Forearm.seek :garage @14:26:00.85 Hand.open @14:26:01.51 Forearm.reconfigure :loose @14:26:01.56 Forearm.seek :retract @14:26:02.59 PSV.dial :mixloop @14:26:02.67 PTV.dial :PRVmixing @14:26:02.75 PRV.dial CBV @14:26:02.78
CBV.dial PRV @14:26:02.86 CTV.dial :air,avoiding: :intake @14:26:02.88 Loading lyfil puck from tube 2 into Collection Clamp @14:26:03.12 SC.reconfigure :tight @14:26:03.17 SC.seek 2 @14:26:06.77 SC.configure :limp @14:26:06.99 Elbow.reconfigure :tight @14:26:07.04 Elbow.seek 2 @14:26:09.39 Forearm.reconfigure :tight @14:26:09.44 Forearm.seek 2 + 450 counts @14:26:10.45 SE.reconfigure :pick @14:26:10.51 SE.seek :beamBreak @14:26:13.47 SE.configure :default @14:26:13.63 Elbow.seek 2 @14:26:13.83 Forearm.seek 2 + 300 counts @14:26:14.09 Forearm.reconfigure :loose @14:26:14.14 Hand.close @14:26:14.71 SE.seek :down @14:26:14.92
Forearm.seek :retract @14:26:15.87 Elbow.seek CC @14:26:17.41 SE.stop @14:26:17.52
Elbow.reconfigure :loose @14:26:17.62 Forearm.reconfigure :tight @14:26:17.67 Forearm.seek CC @14:26:18.97 CC.seek :closed @14:26:23.50 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.11 @14:26:23.51 Hand.open @14:26:24.14 Forearm.reconfigure :loose @14:26:24.18 Forearm.seek :retract @14:26:25.17 CTV.dial CSV,avoiding: :intake @14:26:26.90 CSV.dial CTV @14:26:27.28 PS.seek 2.5ml @14:26:53.75 CS.seek 4.5ml @14:27:08.61 delay 8 seconds @14:27:16.64 PSV.dial :air @14:27:17.11 delay 2 seconds @14:27:19.13 PSV.dial :waste2 @14:27:19.86 PS.seek 0ml @14:27:46.25 CBV.dial between PRV and waste1 @14:27:46.57 CSV.dial :air @14:27:47.02 CS.seek 10ml @14:28:01.83 CSV.dial CTV @14:28:02.29 CS.seek 0ml @14:28:28.92 CBV.dial :waste1 @14:28:29.24 delay 5 seconds @14:28:34.26 CTV.dial :air,avoiding: :intake @14:28:35.66 Storing lyfil puck in Collection Clamp to tube 1 @14:28:35.88 SC.reconfigure :tight @14:28:35.98 SC.seek 1 @14:28:39.60 SC.configure :limp @14:28:39.82 Elbow.reconfigure :tight @14:28:39.86 Elbow.seek 1 @14:28:41.63 Forearm.reconfigure :tight @14:28:41.68 Forearm.seek 1 + 450 counts @14:28:42.75 SE.reconfigure :pick @14:28:42.80 SE.seek :beamBreak @14:28:45.84 SE.configure :default @14:28:46.00 Elbow.reconfigure :limp @14:28:46.05 Forearm.reconfigure :loose @14:28:46.10 Forearm.seek 1 @14:28:46.34 Elbow.hold @14:28:46.40 Elbow.reconfigure :tight @14:28:46.50 SE.seek 0.17 Pucks below Top Plate @14:28:47.41 Forearm.force -20 @14:28:47.45 Forearm.seek 1 + 316 counts @14:28:47.74 Forearm.reconfigure :tight @14:28:47.79 Forearm.seek 1 + 450 counts @14:28:47.98 SE.seek 1.17 Pucks below Top Plate @14:28:48.51 Forearm.reconfigure :loose @14:28:48.56 Forearm.seek :retract @14:28:49.48 Elbow.seek CC @14:28:51.18 Elbow.reconfigure :loose @14:28:51.28 Forearm.seek CC @14:28:52.23 Hand.close @14:28:52.83 CC.seek :open @14:28:57.57 Forearm.seek :retract @14:28:58.59 Elbow.reconfigure :tight @14:28:58.64 Elbow.seek 1 @14:29:00.40 Elbow.reconfigure :limp @14:29:00.45 Forearm.seek 1 @14:29:01.45 Elbow.hold @14:29:01.51 Elbow.reconfigure :tight @14:29:01.56 Hand.open @14:29:02.32 Forearm.force -20 @14:29:02.38 Forearm.seek 1 + 306 counts @14:29:02.74 Forearm.reconfigure :tight @14:29:02.79 Forearm.seek 1 + 450 counts @14:29:03.16 SE.seek :down @14:29:04.76
Forearm.reconfigure :loose @14:29:04.81 Forearm.seek :retract @14:29:05.80 SE.stop @14:29:05.84
PSV.dial :air @14:29:06.71 PS.seek 2.5ml @14:29:33.12 delay 1 second @14:29:34.14 PSV.dial :mixloop @14:29:34.61 delay 1 second @14:29:35.62 PSV.dial :air @14:29:36.09 delay 1 second @14:29:37.11 PSV.dial :mixloop @14:29:37.58 delay 1 second @14:29:38.59 PSV.dial :air @14:29:39.17 delay 1 second @14:29:40.20 PS.seek 0ml @14:30:06.62 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @14:30:06.81 Elbow.seek FlushPuck::Garage @14:30:08.66 Elbow.reconfigure :loose @14:30:08.76 Forearm.seek :garage @14:30:09.81 Hand.close @14:30:10.57 Forearm.seek :retract @14:30:11.56 Elbow.reconfigure :tight @14:30:11.61 Elbow.seek PC @14:30:12.88 Elbow.reconfigure :loose @14:30:12.98 Forearm.reconfigure :tight @14:30:13.03 Forearm.seek PC @14:30:14.30 PC.seek :closed @14:30:18.97 Processing Clamp onSpring/offSpring for 16/26 samples, on/off torque 1.59 @14:30:19.43 Hand.open @14:30:20.14 Forearm.reconfigure :loose @14:30:20.18 Forearm.seek :retract @14:30:21.18 PRV.dial :air @14:30:21.64 PSV.dial :mixloop @14:30:22.16 PS.seek 0.3ml @14:30:26.06 PSV.dial PSR @14:30:27.10 Pulling 0.4ml of PSR.air @14:30:27.11 PSR.select :air @14:30:27.13 PS.seek 0.7ml @14:30:32.03 delay 3 seconds @14:30:35.04 PSR.select :air @14:30:35.07 PS.seek 2.5ml @14:30:54.28 delay 3 seconds @14:30:57.29 PSV.dial :mixloop @14:30:58.34 PTV.dial :mixing @14:30:59.07 PBV.dial :waste2 @14:31:00.00 PS.seek 0ml @14:31:26.37 Garaging FlushPuck in Processing Clamp @14:31:26.44 Elbow.reconfigure :tight @14:31:26.49 Elbow.seek PC @14:31:26.61 Elbow.reconfigure :loose @14:31:26.71 Forearm.seek PC @14:31:27.69 Hand.close @14:31:28.32 PC.seek :open @14:31:33.05 Forearm.seek :retract @14:31:34.01 Elbow.reconfigure :tight @14:31:34.06 Elbow.seek :garage @14:31:35.41 Elbow.reconfigure :loose @14:31:35.51 Forearm.reconfigure :tight @14:31:35.56 Forearm.seek :garage @14:31:36.58 Hand.open @14:31:37.26 Forearm.reconfigure :loose @14:31:37.31 Forearm.seek :retract @14:31:38.25 Loading da puck from tube 2 into Processing Clamp @14:31:38.49 SC.reconfigure :tight @14:31:38.54 SC.seek 2 @14:31:42.16 SC.configure :limp @14:31:42.83 Elbow.reconfigure :tight @14:31:42.88 Elbow.seek 2 @14:31:44.79 Forearm.reconfigure :tight @14:31:44.84 Forearm.seek 2 + 450 counts @14:31:45.88 SE.reconfigure :pick @14:31:45.94 SE.seek :beamBreak @14:31:49.10 SE.configure :default @14:31:49.26 Elbow.seek 2 @14:31:49.60 Forearm.seek 2 + 300 counts @14:31:49.84 Forearm.reconfigure :loose @14:31:49.89 Hand.close @14:31:50.46 SE.seek :down @14:31:50.66
Forearm.seek :retract @14:31:51.60 Elbow.seek PC @14:31:53.19 SE.stop @14:31:54.09
Elbow.reconfigure :loose @14:31:54.20 Forearm.reconfigure :tight @14:31:54.24 Forearm.seek PC @14:31:55.55 PC.seek :closed @14:32:00.21 Processing Clamp onSpring/offSpring for 16/26 samples, on/off torque 1.60 @14:32:00.22 Hand.open @14:32:00.88 Forearm.reconfigure :loose @14:32:00.93 Forearm.seek :retract @14:32:01.89 Heating Puck.from 2, :da in Processing Clamp to 28C for 2:00:00 @14:32:02.38 PH.seek 28C,5:00 @14:32:27.81 PH.hold 2:00:00,28C @14:32:27.86 delay 1:30 @14:33:57.91 PSR.select :air @14:33:57.92 PSV.dial PSR @14:33:58.98 Pulling 0.5ml of PSR.wash PSR.select :wash @14:33:59.11 PS.seek 0.5ml @14:34:05.02 delay 5 seconds @14:34:10.04 PSR.select :air @14:34:10.16 PS.seek 2ml @14:34:26.27 PTV.dial :puck @14:34:27.01 PSV.dial PTV @14:34:27.76 PBV.dial :waste1 @14:34:29.13 PS.seek 1.5ml @14:34:35.05 delay 3 seconds @14:34:38.07 PBV.dial between PSV and waste1 @14:34:38.42 PS.seek 0ml @14:34:54.55 delay 5:00 @14:39:54.57 recoverPSRreagent08 @14:39:54.58 PTV.dial :puck @14:39:54.65 PBV.dial :waste1 @14:39:55.02 delay 30 seconds @14:40:25.04 PBV.dial between PSV and waste1 @14:40:25.42 PSR.select :air @14:40:25.44 PSV.dial PSR @14:40:26.19 PS.reconfigure :fast @14:40:26.24 PS.seek 2.5ml @14:40:49.92 PSV.dial PTV @14:40:50.67 delay 5 seconds @14:40:55.69 PSV.dial PSR @14:40:56.89 delay 5 seconds @14:41:01.91 PSV.dial PTV @14:41:02.66 delay 5 seconds @14:41:07.68 PSV.dial :air @14:41:08.43 PS.seek 0ml @14:41:31.55 PTV.dial :PRVmixing,via: PRV @14:41:32.86 PSV.dial :mixloop @14:41:33.33 PRV.dial :ab1 @14:41:34.08 PS.reconfigure :slow @14:41:34.13 PS.seek 0.15ml @14:41:37.76 delay 2 seconds @14:41:39.78 CBV.dial PRV @14:41:39.81
PRV.dial CBV @14:41:40.38 PS.seek 0.34ml @14:41:44.76 delay 2 seconds @14:41:46.78 CBV.dial between PRV and PSV @14:41:47.10 PRV.dial :air @14:41:47.55 PS.seek 0.9ml @14:41:59.51 PRV.dial :waste2 @14:42:00.25 PS.seek 0.5ml @14:42:08.95 addPMLReagent08 @14:42:08.96 PSV.dial :air @14:42:09.42 PS.reconfigure :default @14:42:09.47 PS.seek 2ml @14:42:25.67 PSV.dial :PML @14:42:26.14 PTV.dial :mixing @14:42:26.87 PBV.dial :waste1 @14:42:27.31 PS.seek 1.85ml @14:42:29.67 PBV.dial between PSV and waste1 @14:42:30.03 PS.seek 0ml @14:42:49.82 delay 2 seconds @14:42:51.84 Beginning samplePassC @14:42:51.86 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:42:52.04 Elbow.reconfigure :tight @14:42:52.09 Elbow.seek FlushPuck::Garage @14:42:53.62 Elbow.reconfigure :loose @14:42:53.72 Forearm.seek :garage @14:42:54.75 Hand.close @14:42:55.44 Forearm.seek :retract @14:42:56.42 Elbow.reconfigure :tight @14:42:56.47 Elbow.seek CC @14:42:59.82 Elbow.reconfigure :loose @14:42:59.93 Forearm.reconfigure :tight @14:42:59.98 Forearm.seek CC @14:43:01.18 CC.seek :closed @14:43:05.70 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.34 @14:43:05.77 Hand.open @14:43:06.50 Forearm.reconfigure :loose @14:43:06.55 Forearm.seek :retract @14:43:07.53 PSV.dial PSR @14:43:08.56 Pulling 1.5ml of PSR.flush @14:43:08.57 PSR.select :flush @14:43:08.69 PS.seek 1.5ml @14:43:24.89 delay 5 seconds @14:43:29.91 PSV.dial :waste1 @14:43:30.38 PS.seek 0ml @14:43:46.54 PSV.dial PSR @14:43:47.01 PSR.select :air @14:43:47.13 PS.seek 2.5ml @14:44:13.51 PRV.dial CBV,avoiding: :ab2 @14:44:13.54
CBV.dial PRV @14:44:15.13 PSV.dial :mixloop @14:44:16.15 CTV.dial :air,avoiding: :intake @14:44:16.39 PTV.dial :PRVmixing @14:44:17.16 PS.seek 0ml @14:44:43.51 PSV.dial :air @14:44:43.97 PS.seek 2.5ml @14:45:10.32 PSV.dial :mixloop @14:45:10.80 PS.seek 0ml @14:45:37.13 PSV.dial :air @14:45:37.61 PS.seek 2.5ml @14:46:04.41 PSV.dial :mixloop @14:46:04.89 PS.seek 0ml @14:46:31.29 Garaging FlushPuck in Collection Clamp @14:46:31.37 Elbow.reconfigure :tight @14:46:31.41 Elbow.seek CC @14:46:31.68 Elbow.reconfigure :loose @14:46:31.78 Forearm.seek CC @14:46:32.82 Hand.close @14:46:33.44 CC.seek :open @14:46:38.09 Forearm.seek :retract @14:46:39.09 Elbow.reconfigure :tight @14:46:39.14 Elbow.seek :garage @14:46:41.99 Elbow.reconfigure :loose @14:46:42.09 Forearm.reconfigure :tight @14:46:42.14 Forearm.seek :garage @14:46:43.17 Hand.open @14:46:43.88 Forearm.reconfigure :loose @14:46:43.92 Forearm.seek :retract @14:46:44.94 delayUntil "14:50:51.83" @14:46:44.96 Whole Cell Archive sampling 50ml @14:46:44.98 Loading wcr puck from tube 2 into Collection Clamp @14:46:45.23 SC.reconfigure :tight @14:46:45.28 SC.seek 2 @14:46:45.58 SC.configure :limp @14:46:45.81 Elbow.reconfigure :tight @14:46:45.86 Elbow.seek 2 @14:46:47.75 Forearm.reconfigure :tight @14:46:47.80 Forearm.seek 2 + 450 counts @14:46:48.87 SE.reconfigure :pick @14:46:48.93 SE.seek :beamBreak @14:46:51.97 SE.configure :default @14:46:52.14 Elbow.seek 2 @14:46:52.33 Forearm.seek 2 + 300 counts @14:46:52.71 Forearm.reconfigure :loose @14:46:52.76 Hand.close @14:46:53.44 Puck::Warning in WCR -- Loaded Last remaining Puck (from Tube 2) @14:46:53.47 SE.seek :down @14:46:53.68 Forearm.seek :retract @14:46:54.65 Elbow.seek CC @14:46:56.26 SE.stop @14:46:56.29 Elbow.reconfigure :loose @14:46:56.43 Forearm.reconfigure :tight @14:46:56.49 Forearm.seek CC @14:46:57.84 CC.seek :closed @14:47:02.38 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.22 @14:47:02.39 Hand.open @14:47:03.12 Forearm.reconfigure :loose @14:47:03.17 Forearm.seek :retract @14:47:04.12 collectSample 50ml w/bubble point of 35psi @14:47:04.14 Priming 7ml @14:47:04.15 Intake.select :close @14:47:04.16 Exhaust.select :close @14:47:04.17 SS.hold @14:47:04.38 Calibrating pressure sensors @14:47:04.39 CTV.dial :air @14:47:04.47 SSV.dial :spit,avoiding: :exhaust @14:47:05.04 SS.reconfigure :fast @14:47:05.10 SS.seek 5ml @14:47:07.79 SSV.dial CBV @14:47:07.82 CBV.dial SSV @14:47:09.16 delay 3.8 seconds @14:47:13.09 Sensor limits: Intake max 116.5psia; Outlet max 109.8psia @14:47:13.10 SSV.dial :spit @14:47:14.43 SS.seek 0ml @14:47:17.52 SSV.dial CBV @14:47:18.85 CTV.dial :intake @14:47:19.64 SSV.dial CBV @14:47:19.67 CBV.dial SSV @14:47:19.86 Allowing 40s for 7.0ml of remaining 7.0ml @14:47:19.88 Intake.select :open @14:47:22.05 Intake @15.4psia, vacuum from 9.9 to 12.9psi @14:47:22.07 SS.reconfigure :inhaling 2.20ml/s @14:47:22.12 SS.seek 7ml @14:47:27.80 Stroke completed -- waiting for pressure to equalize ... @14:47:27.81 SS.reconfigure :exhale @14:47:27.86 SS.hold @14:47:33.52 Intake.select :close @14:47:33.67 SSV.dial :exhaust @14:47:34.44 Exhaust.select :open @14:47:34.56 SS.seek 0ml @14:47:39.14 Exhaust.select :close @14:47:39.26 Sampling 50.0ml @14:47:39.27 Intake.select :close @14:47:39.28 Exhaust.select :close @14:47:39.29 SS.reconfigure :default @14:47:39.34 SS.hold @14:47:39.56 CTV.dial :intake @14:47:39.80 SSV.dial CBV @14:47:39.84 CBV.dial SSV @14:47:40.58 Allowing 130s for 25.0ml of remaining 50.0ml @14:47:40.60 Intake.select :open @14:47:42.76 Intake @15.8psia, vacuum from 10.0 to 13.0psi @14:47:42.78 SS.reconfigure :inhaling 2.20ml/s @14:47:42.83 SS.seek 25ml @14:47:55.62 Vacuum too high -- waiting up to 40 seconds @14:47:55.63 SS.reconfigure :exhale @14:47:55.69 SS.hold @14:47:56.38 Intake @12.8psia, vacuum from 7.3 to 10.3psi @14:47:56.40 SS.reconfigure :inhaling 1.76ml/s @14:47:56.45 SS.seek 25ml @14:47:59.22 Stroke completed -- waiting for pressure to equalize ... @14:47:59.23 SS.reconfigure :exhale @14:47:59.28 SS.hold @14:48:04.14 Intake.select :close @14:48:04.30 SSV.dial :exhaust @14:48:05.06 Exhaust.select :open @14:48:05.18 SS.seek 0ml @14:48:14.39 Exhaust.select :close @14:48:14.51 SSV.dial CBV @14:48:14.54 CBV.dial SSV @14:48:15.31 Allowing 130s for 25.0ml of remaining 25.0ml @14:48:15.33 Intake.select :open @14:48:17.49 Intake @15.9psia, vacuum from 10.0 to 13.0psi @14:48:17.51 SS.reconfigure :inhaling 1.76ml/s @14:48:17.56 SS.seek 25ml @14:48:35.23 Stroke completed -- waiting for pressure to equalize ... @14:48:35.25 SS.reconfigure :exhale @14:48:35.29 SS.hold @14:48:40.83 Intake.select :close @14:48:40.99 SSV.dial :exhaust @14:48:41.75 Exhaust.select :open @14:48:41.87 SS.seek 0ml @14:48:50.59 Exhaust.select :close @14:48:50.71 Sampled 50.0ml @14:48:50.73 Drying puck before fixing for 4:00 @14:48:50.74 CBV.dial :waste2b @14:48:51.48 delay 1:00 @14:49:51.51 CSV.dial :air @14:49:51.97 CS.reconfigure :fast @14:49:52.03 CS.seek 10ml @14:50:16.65 Pumping wcr puck from tube 2 up to 35psi... @14:50:16.67 CBV.dial SSV @14:50:17.92 CBV.dial between CSV and waste2b @14:50:19.34 CSV.dial between air and CTV @14:50:19.68 CS.seek 7.303ml @14:50:26.72 CTV.dial CSV,avoiding: :air @14:50:26.77 CSV.dial CTV @14:50:27.55 CS.reconfigure :pressurize @14:50:27.60 CS.seek 0ml @14:50:43.02 CBV.dial :waste2b @14:50:43.34 CS.reconfigure :default @14:50:43.40 CS.seek 0ml @14:50:48.00 delay 1:00 @14:50:51.84 recoverPRVreagent08 @14:50:51.85 PTV.dial :mixing @14:50:52.59 PBV.dial :waste1 @14:50:52.96 delay 30 seconds @14:51:22.98 PBV.dial between PSV and waste1 @14:51:23.35 PSV.dial :air @14:51:23.82 PS.reconfigure :fast @14:51:23.87 PS.seek 2.5ml @14:51:47.56 PSV.dial :mixloop @14:51:48.03 delay 2 seconds @14:51:48.05 CTV.dial :air,avoiding: :intake @14:51:49.38 Fix #1 of 1 (with RNAlater) for 4:00 @14:51:49.39 CSV.dial CSR @14:51:49.82 Pulling 2ml of CSR.RNAlater @14:51:49.83 CSR.select :RNAlater @14:51:49.95 CS.seek 2ml @14:51:50.05 PSV.dial :air @14:51:50.52 delay 2 seconds @14:51:52.53 PSV.dial :mixloop @14:51:53.00 delay 2 seconds @14:51:55.02 PSV.dial :air @14:51:55.49 PS.seek 0ml @14:51:55.87 delay 5 seconds @14:52:00.89 CSR.select :air @14:52:01.00 CS.reconfigure :fast @14:52:01.05 CS.seek 10ml @14:52:18.66 washCycleDA #1 of 2 @14:52:18.67 PSR.select :air @14:52:18.69 PSV.dial PSR @14:52:20.00 Pulling 0.5ml of PSR.wash @14:52:20.01 PSR.select :wash @14:52:20.13 PS.reconfigure :default @14:52:20.17 PS.seek 0.5ml @14:52:20.78 Pumping wcr puck from tube 2 up to 35psi... @14:52:20.80 CBV.dial SSV @14:52:22.03 CBV.dial between CSV and waste2b @14:52:23.00 CSV.dial between air and CTV @14:52:23.61 CS.seek 7.286ml @14:52:26.16 delay 5 seconds @14:52:31.09 CTV.dial CSV,avoiding: :air @14:52:31.13 CSV.dial CTV @14:52:31.24 PSR.select :air @14:52:31.36 PS.seek 2ml @14:52:32.50 CS.reconfigure :pressurize @14:52:32.56 CS.seek 0ml @14:52:47.50 PTV.dial :puck @14:52:48.23 PSV.dial PTV @14:52:48.97 PBV.dial :waste1 @14:52:49.39 PS.seek 1.5ml @14:52:49.48 delay 4:00 @14:52:55.31 delay 3 seconds @14:52:58.33 PBV.dial between PSV and waste1 @14:52:58.69 PS.seek 0ml @14:53:15.06 delay 2:00 @14:55:15.09 recoverPSRreagent08 @14:55:15.10 PTV.dial :puck @14:55:15.11 PBV.dial :waste1 @14:55:15.47 delay 30 seconds @14:55:45.49 PBV.dial between PSV and waste1 @14:55:45.92 PSR.select :air @14:55:45.94 PSV.dial PSR @14:55:46.69 PS.reconfigure :fast @14:55:46.74 PS.seek 2.5ml @14:56:10.62 PSV.dial PTV @14:56:11.37 delay 5 seconds @14:56:16.39 PSV.dial PSR @14:56:17.15 delay 5 seconds @14:56:22.17 PSV.dial PTV @14:56:22.92 delay 5 seconds @14:56:27.94 PSV.dial :air @14:56:28.69 PS.seek 0ml @14:56:49.50 CBV.dial :waste2b @14:56:49.84 CS.reconfigure :default @14:56:49.89 CS.seek 0ml @14:56:52.00 washCycleDA #2 of 2 @14:56:52.01 PSR.select :air @14:56:52.02 PSV.dial PSR @14:56:52.99 delay 1:00 @14:56:53.34 Pulling 0.5ml of PSR.wash @14:56:53.35 PSR.select :wash @14:56:53.47 PS.reconfigure :default @14:56:53.52 PS.seek 0.5ml @14:56:59.53 delay 5 seconds @14:57:04.55 PSR.select :air @14:57:04.67 PS.seek 2ml @14:57:20.77 PTV.dial :puck @14:57:20.79 PSV.dial PTV @14:57:21.52 PBV.dial :waste1 @14:57:21.96 PS.seek 1.5ml @14:57:27.90 delay 3 seconds @14:57:30.92 PBV.dial between PSV and waste1 @14:57:31.28 PS.seek 0ml @14:57:47.40 delay 2:00 @14:57:53.01 CTV.dial :air,avoiding: :intake @14:59:47.42 recoverPSRreagent08 @14:59:47.43 PTV.dial :puck @14:59:47.44 PBV.dial :waste1 @14:59:47.82 delay 30 seconds @15:00:17.84 PBV.dial between PSV and waste1 @15:00:18.19 PSR.select :air @15:00:18.21 PSV.dial PSR @15:00:18.96 PS.reconfigure :fast @15:00:19.01 PS.seek 2.5ml @15:00:42.68 PSV.dial PTV @15:00:43.43 delay 5 seconds @15:00:48.45 PSV.dial PSR @15:00:49.21 delay 5 seconds @15:00:54.23 PSV.dial PTV @15:00:54.97 delay 5 seconds @15:01:00.00 PSV.dial :air @15:01:00.75 PS.seek 0ml @15:01:24.02 getPMLReagent08 @15:01:24.03 PRV.dial :air @15:01:24.49 delay 5 seconds @15:01:29.51 PTV.dial :PRVmixing @15:01:30.80 delay 5 seconds @15:01:35.82 PRV.dial :ab2 @15:01:36.27 PTV.dial :PRVmixing @15:01:36.35 PSV.dial :mixloop @15:01:36.82 PS.reconfigure :default @15:01:36.87 PS.seek 0.3ml @15:01:40.80 delay 2 seconds @15:01:42.82 PRV.dial :air @15:01:43.35 PS.seek 0.55ml @15:01:46.77 addPMLReagent08 @15:01:46.78 PSV.dial :air @15:01:47.37 PS.seek 2ml @15:02:03.02 PSV.dial :PML @15:02:03.47 PTV.dial :mixing @15:02:04.22 PBV.dial :waste1 @15:02:04.66 PS.seek 1.85ml @15:02:07.14 PBV.dial between PSV and waste1 @15:02:07.51 PS.seek 0ml @15:02:27.39 delay 2 seconds @15:02:29.41 delay 8:00 @15:10:29.44 recoverPRVreagent08 @15:10:29.45 PTV.dial :mixing @15:10:29.52 PBV.dial :waste1 @15:10:29.90 delay 30 seconds @15:10:59.93 PBV.dial between PSV and waste1 @15:11:00.29 PSV.dial :air @15:11:00.76 PS.reconfigure :fast @15:11:00.81 PS.seek 2.5ml @15:11:24.51 PSV.dial :mixloop @15:11:24.98 delay 2 seconds @15:11:27.00 PSV.dial :air @15:11:27.46 delay 2 seconds @15:11:29.48 PSV.dial :mixloop @15:11:29.95 delay 2 seconds @15:11:31.96 PSV.dial :air @15:11:32.44 PS.seek 0ml @15:11:55.63 washCycleDA #1 of 3 @15:11:55.64 PSR.select :air @15:11:55.66 PSV.dial PSR @15:11:56.98 Pulling 0.5ml of PSR.wash @15:11:56.99 PSR.select :wash @15:11:57.52 PS.reconfigure :default @15:11:57.63 PS.seek 0.5ml @15:12:03.63 delay 5 seconds @15:12:08.65 PSR.select :air @15:12:08.77 PS.seek 2ml @15:12:24.97 PTV.dial :puck @15:12:25.69 PSV.dial PTV @15:12:26.43 PBV.dial :waste1 @15:12:26.87 PS.seek 1.5ml @15:12:32.79 delay 3 seconds @15:12:35.81 PBV.dial between PSV and waste1 @15:12:36.17 PS.seek 0ml @15:12:52.35 delay 2:00 @15:14:52.38 recoverPSRreagent08 @15:14:52.39 PTV.dial :puck @15:14:52.40 PBV.dial :waste1 @15:14:52.77 delay 30 seconds @15:15:22.80 PBV.dial between PSV and waste1 @15:15:23.16 PSR.select :air @15:15:23.18 PSV.dial PSR @15:15:23.93 PS.reconfigure :fast @15:15:23.98 PS.seek 2.5ml @15:15:47.69 PSV.dial PTV @15:15:48.44 delay 5 seconds @15:15:53.46 PSV.dial PSR @15:15:54.22 delay 5 seconds @15:15:59.24 PSV.dial PTV @15:15:59.99 delay 5 seconds @15:16:05.01 PSV.dial :air @15:16:05.88 PS.seek 0ml @15:16:29.07 washCycleDA #2 of 3 @15:16:29.08 PSR.select :air @15:16:29.09 PSV.dial PSR @15:16:30.53 Pulling 0.5ml of PSR.wash @15:16:30.54 PSR.select :wash @15:16:30.67 PS.reconfigure :default @15:16:30.71 PS.seek 0.5ml @15:16:36.63 delay 5 seconds @15:16:41.65 PSR.select :air @15:16:41.77 PS.seek 2ml @15:16:57.97 PTV.dial :puck @15:16:57.98 PSV.dial PTV @15:16:58.72 PBV.dial :waste1 @15:16:59.15 PS.seek 1.5ml @15:17:05.13 delay 3 seconds @15:17:08.14 PBV.dial between PSV and waste1 @15:17:08.51 PS.seek 0ml @15:17:24.63 delay 2:00 @15:19:24.66 recoverPSRreagent08 @15:19:24.67 PTV.dial :puck @15:19:24.68 PBV.dial :waste1 @15:19:25.05 delay 30 seconds @15:19:55.08 PBV.dial between PSV and waste1 @15:19:55.45 PSR.select :air @15:19:55.47 PSV.dial PSR @15:19:56.22 PS.reconfigure :fast @15:19:56.27 PS.seek 2.5ml @15:20:20.03 PSV.dial PTV @15:20:20.78 delay 5 seconds @15:20:25.80 PSV.dial PSR @15:20:26.55 delay 5 seconds @15:20:31.57 PSV.dial PTV @15:20:32.31 delay 5 seconds @15:20:37.76 PSV.dial :air @15:20:38.71 PS.seek 0ml @15:21:01.94 washCycleDA #3 of 3 @15:21:01.95 PSR.select :air @15:21:01.97 PSV.dial PSR @15:21:03.40 Pulling 0.5ml of PSR.wash @15:21:03.41 PSR.select :wash @15:21:03.53 PS.reconfigure :default @15:21:03.58 PS.seek 0.5ml @15:21:09.50 delay 5 seconds @15:21:14.52 PSR.select :air @15:21:14.64 PS.seek 2ml @15:21:30.84 PTV.dial :puck @15:21:30.85 PSV.dial PTV @15:21:31.59 PBV.dial :waste1 @15:21:32.02 PS.seek 1.5ml @15:21:37.96 delay 3 seconds @15:21:40.98 PBV.dial between PSV and waste1 @15:21:41.34 PS.seek 0ml @15:21:57.50 delay 2:00 @15:23:57.53 recoverPSRreagent08 PTV.dial :puck @15:23:57.55 PBV.dial :waste1 @15:23:57.91 delay 30 seconds @15:24:27.94 PBV.dial between PSV and waste1 @15:24:28.32 PSR.select :air @15:24:28.34 PSV.dial PSR @15:24:29.09 PS.reconfigure :fast @15:24:29.14 PS.seek 2.5ml @15:24:52.83 PSV.dial PTV @15:24:53.59 delay 5 seconds @15:24:58.61 PSV.dial PSR @15:24:59.35 delay 5 seconds @15:25:04.37 PSV.dial PTV @15:25:05.13 delay 5 seconds @15:25:10.15 PSV.dial :air @15:25:10.90 PS.seek 0ml @15:25:34.08 Gate.power :camera,:ON @15:25:34.10 Mixing DA Substrates @15:25:34.11 PSR.select :air @15:25:34.13 PSV.dial PSR @15:25:35.53 Pulling 0.25ml of PSR.sub1 @15:25:35.58 PSR.select :sub1 @15:25:35.78 PS.reconfigure :default @15:25:35.90 PS.seek 0.25ml @15:25:39.25 delay 5 seconds @15:25:44.27 Pulling 0.25ml of PSR.sub2 @15:25:44.29 PSR.select :sub2 @15:25:44.41 PS.seek 0.5ml @15:25:47.77 delay 5 seconds @15:25:52.79 PSR.select :air @15:25:52.91 PS.seek 2ml @15:26:09.11 PTV.dial :puck @15:26:09.13 PSV.dial PTV @15:26:09.88 PBV.dial :waste1 @15:26:10.32 PS.seek 1.5ml @15:26:16.21 delay 3 seconds @15:26:19.23 PBV.dial between PSV and waste1 @15:26:19.59 PS.seek 0ml @15:26:35.67 delay 10 seconds @15:26:45.70 recoverPSRreagent08 @15:26:45.71 PTV.dial :puck @15:26:45.72 PBV.dial :waste1 @15:26:46.08 delay 30 seconds @15:27:16.11 PBV.dial between PSV and waste1 @15:27:16.47 PSR.select :air @15:27:16.49 PSV.dial PSR @15:27:17.26 PS.reconfigure :fast @15:27:17.81 PS.seek 2.5ml @15:27:41.96 PSV.dial PTV @15:27:42.72 delay 5 seconds @15:27:47.74 PSV.dial PSR @15:27:48.51 delay 5 seconds @15:27:53.53 PSV.dial PTV @15:27:54.41 delay 5 seconds @15:27:59.43 PSV.dial :air @15:28:00.23 PS.seek 0ml @15:28:24.08 Additional air clear @15:28:24.09 PSR.select :air @15:28:24.11 PSV.dial PSR @15:28:25.46 Pulling 2ml of PSR.air @15:28:25.47 PSR.select :air @15:28:25.49 PS.reconfigure :default @15:28:25.54 PS.seek 2ml @15:28:46.86 delay 5 seconds @15:28:51.88 PTV.dial :puck @15:28:51.90 PSV.dial PTV @15:28:52.64 PBV.dial :waste1 @15:28:53.08 PS.seek 1.5ml @15:28:59.05 delay 3 seconds @15:29:02.07 PBV.dial between PSV and waste1 @15:29:02.44 delay 1 second @15:29:03.46 PSV.dial :waste1 @15:29:04.52 PS.seek 0ml @15:29:20.69 Processing Puck Heater at 27.9C before imaging @15:29:20.70 PH.coast @15:29:20.82 Imaging da puck in Processing Clamp while storing it to tube 1 @15:29:21.00 SC.reconfigure :tight @15:29:21.05 SC.seek 1 @15:29:24.65 SC.configure :limp @15:29:24.87 Elbow.reconfigure :tight @15:29:24.92 Elbow.seek 1 @15:29:27.61 Forearm.reconfigure :tight @15:29:27.66 Forearm.seek 1 + 450 counts @15:29:28.69 SE.reconfigure :pick @15:29:28.75 SE.seek :beamBreak @15:29:31.81 SE.configure :default @15:29:31.97 Elbow.reconfigure :limp @15:29:32.03 Forearm.reconfigure :loose @15:29:32.07 Forearm.seek 1 @15:29:32.36 Elbow.hold @15:29:32.43 Elbow.reconfigure :tight @15:29:32.54 SE.seek 1.17 Pucks below Top Plate @15:29:33.43 Forearm.force -20 @15:29:33.54 Forearm.seek 1 + 309 counts @15:29:33.94 Forearm.reconfigure :tight @15:29:33.99 Forearm.seek 1 + 450 counts @15:29:34.21 SE.seek 2.17 Pucks below Top Plate @15:29:34.77 Forearm.reconfigure :loose @15:29:34.83 Forearm.seek :retract @15:29:35.76 Elbow.seek PC @15:29:38.46 Elbow.reconfigure :loose @15:29:38.56 Forearm.seek PC @15:29:39.55 Hand.close @15:29:40.15 PC.seek :open @15:29:44.94 Forearm.seek :retract @15:29:45.91 Elbow.reconfigure :tight @15:29:45.96 Elbow.seek 1 @15:29:48.50 Forearm.reconfigure :tight @15:29:48.55 Forearm.seek 1 + 450 counts @15:29:49.55 Hand.open @15:29:50.22 SE.seek 1.82 Pucks below Top Plate @15:29:50.50 SE.reconfigure :seal @15:29:50.55 SE.seek 0.85 Pucks below Top Plate @15:29:51.11 Lights, camera, ACTION! @15:29:51.15 PuckCamera: snap -bin=2,2 -auto /var/log/esp/da24jan1915h50ml.tif @15:29:58.31 SX HX9/16 (USB:05): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 2.835 seconds /var/log/esp/da24jan1915h50ml.tif: TIFF Upload Complete @15:29:58.34 PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da24jan1915h50ml15s.tif @15:30:10.60 Waiting >20 seconds for da to relinquish FlushPuck @15:30:13.98 SX HX9/16 (USB:05): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15 seconds /var/log/esp/midres/da24jan1915h50ml15s.tif: TIFF Upload Complete @15:30:14.01 PuckCamera: snap -bin=2,2 20.0 /var/log/esp/midres/da24jan1915h50ml20s.tif @15:30:34.62 SX HX9/16 (USB:05): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 20 seconds /var/log/esp/midres/da24jan1915h50ml20s.tif: TIFF Upload Complete @15:30:34.64 Gate.power :camera,:OFF @15:30:34.66 DAfans.email "2.835 sec auto-exposure",Subject: "da24jan1915h50ml images!" @15:30:35.09 Elbow.seek 1 @15:30:35.34 Forearm.seek 1 + 450 counts @15:30:35.50 SE.reconfigure :gently @15:30:35.57 SE.seek 1.57 Pucks below Top Plate @15:30:36.83 Elbow.reconfigure :limp @15:30:36.89 Forearm.reconfigure :loose @15:30:36.94 Forearm.seek 1 @15:30:37.21 Elbow.hold @15:30:37.34 Elbow.reconfigure :tight @15:30:37.40 SE.reconfigure :default @15:30:37.45 SE.seek 1.75 Pucks below Top Plate @15:30:37.83 Forearm.force -20 @15:30:37.87 Forearm.seek 1 + 310 counts @15:30:38.48 Forearm.reconfigure :tight @15:30:38.61 Forearm.seek 1 + 450 counts @15:30:38.94 SE.seek :down @15:30:40.21 Forearm.reconfigure :loose @15:30:40.35 Forearm.seek :retract @15:30:41.55 SE.stop @15:30:41.78 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @15:30:42.48 Elbow.seek FlushPuck::Garage @15:30:44.47 Elbow.reconfigure :loose @15:30:44.68 Forearm.seek :garage @15:30:45.71 Hand.close @15:30:46.80 Forearm.seek :retract @15:30:47.85 Elbow.reconfigure :tight @15:30:47.96 Elbow.seek PC @15:30:49.38 Elbow.reconfigure :loose @15:30:49.63 Forearm.reconfigure :tight @15:30:49.78 Forearm.seek PC @15:30:51.55 PC.seek :closed @15:30:56.40 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.65 @15:30:56.56 Hand.open @15:30:57.23 upsync @15:30:57.34 Forearm.reconfigure :loose @15:30:57.39 Forearm.seek :retract @15:30:58.33 PSR.select :air @15:30:58.36 Storing wcr puck in Collection Clamp to tube 1 @15:30:58.38 PSV.dial PSR @15:30:58.63 SC.reconfigure :tight @15:30:58.68 SC.seek 1 @15:30:58.91 Pulling 0.5ml of PSR.flush @15:30:58.92 PSR.select :flush @15:30:58.98 SC.configure :limp @15:30:59.12 PS.seek 0.5ml @15:30:59.31 Elbow.reconfigure :tight @15:30:59.36 Elbow.seek 1 @15:31:01.99 Forearm.reconfigure :tight @15:31:02.04 Forearm.seek 1 + 450 counts @15:31:03.07 SE.reconfigure :pick @15:31:03.13 SE.seek :beamBreak @15:31:05.08 delay 5 seconds @15:31:06.08 SE.configure :default @15:31:06.25 Elbow.reconfigure :limp @15:31:06.30 Forearm.reconfigure :loose @15:31:06.35 Forearm.seek 1 @15:31:06.61 Elbow.hold @15:31:06.76 Elbow.reconfigure :tight @15:31:06.88 SE.seek 2.16 Pucks below Top Plate @15:31:07.36 Forearm.force -20 @15:31:07.41 Forearm.seek 1 + 303 counts @15:31:08.08 Forearm.reconfigure :tight @15:31:08.13 Forearm.seek 1 + 450 counts @15:31:08.32 SE.seek 3.16 Pucks below Top Plate @15:31:08.84 Forearm.reconfigure :loose @15:31:08.90 Forearm.seek :retract @15:31:09.87 Elbow.seek CC @15:31:10.09 PSR.select :air @15:31:10.22 PS.seek 2.5ml @15:31:11.52 Elbow.reconfigure :loose @15:31:11.62 Forearm.seek CC @15:31:12.58 Hand.close @15:31:13.15 CC.seek :open @15:31:17.95 Forearm.seek :retract @15:31:18.91 Elbow.reconfigure :tight @15:31:18.97 Elbow.seek 1 @15:31:20.76 Elbow.reconfigure :limp @15:31:20.82 Forearm.seek 1 @15:31:21.88 Elbow.hold @15:31:21.95 Elbow.reconfigure :tight @15:31:22.00 Hand.open @15:31:22.78 Forearm.force -20 @15:31:22.82 Forearm.seek 1 + 316 counts @15:31:23.19 Forearm.reconfigure :tight @15:31:23.24 Forearm.seek 1 + 450 counts @15:31:24.05 SE.seek :down @15:31:25.19 Forearm.reconfigure :loose @15:31:25.24 Forearm.seek :retract @15:31:26.57 SE.stop @15:31:26.62
Starting finalPurge @15:31:31.80 delay 1 second @15:31:32.82 PRV.dial :waste2,avoiding: :ab2 @15:31:34.67 PTV.dial :PRVmixing @15:31:35.98 PSV.dial :mixloop @15:31:37.03 PS.seek :empty @15:31:46.58
Waiting >20 seconds for psFlush to relinquish FlushPuck @15:32:03.45 PSR.select :air @15:32:03.47 PSV.dial PSR @15:32:04.51 Pulling 0.5ml of PSR.flush @15:32:04.52 PSR.select :flush @15:32:04.65 PS.seek 0.5ml @15:32:10.64 delay 5 seconds @15:32:15.66 PSR.select :air @15:32:15.78 PS.seek 2.5ml @15:32:37.11 delay 1 second @15:32:38.13 PBV.dial :waste1 @15:32:38.48 PTV.dial :mixing @15:32:39.23 PSV.dial :mixloop @15:32:40.41 PS.seek :empty @15:33:06.82 PSV.dial PSR @15:33:07.86 PSR.select :flush @15:33:07.98 PS.seek 1ml @15:33:19.04 PSR.select :air @15:33:19.16 PS.seek 2.5ml @15:33:35.35 PSV.dial PTV @15:33:36.11 PTV.dial :puck @15:33:36.84 PBV.dial :waste1 @15:33:36.92 PS.seek 0ml @15:34:03.42 PSV.dial PSR @15:34:04.17 PSR.select :flush @15:34:04.29 PS.seek 0.5ml @15:34:10.26 PSR.select :air @15:34:10.38 PS.seek 2.5ml @15:34:31.67 PSV.dial PTV @15:34:32.42 PBV.dial PSV @15:34:32.98 PS.seek 0ml @15:34:59.32 PSV.dial PBV @15:34:59.91 PS.seek 2.5ml @15:35:26.32 PSV.dial :waste1 @15:35:27.08 PS.seek 0ml @15:35:53.57 PSV.dial PSR @15:35:54.60 PSR.select :air @15:35:54.63 PS.seek 2.5ml @15:36:20.98 PSV.dial PTV @15:36:21.73 PBV.dial PSV @15:36:21.92 PS.seek 0ml @15:36:48.44 PSV.dial PBV @15:36:49.03 PS.seek 2.5ml @15:37:15.38 PSV.dial :waste1 @15:37:16.14 PS.seek 0ml @15:37:42.44 PSV.dial PSR @15:37:42.90 PSR.select :flush @15:37:43.02 PS.seek 1ml @15:37:54.13 PSR.select :air @15:37:54.25 PS.seek 2.5ml @15:38:10.54 PSV.dial PTV @15:38:11.27 PTV.dial :puck @15:38:11.35 PBV.dial :waste1 @15:38:11.89 PS.seek 0ml @15:38:38.16 PSV.dial PSR @15:38:38.91 PSR.select :flush @15:38:39.04 PS.seek 0.5ml @15:38:45.03 PSR.select :air @15:38:45.16 PS.seek 2.5ml @15:39:06.44 PSV.dial PTV @15:39:07.19 PBV.dial PSV @15:39:07.74 PS.seek 0ml @15:39:34.13 PSV.dial PBV @15:39:34.59 PS.seek 2.5ml @15:40:01.10 PSV.dial :waste1 @15:40:01.84 PS.seek 0ml @15:40:28.22 PSV.dial PSR @15:40:28.67 PSR.select :air @15:40:28.70 PS.seek 2.5ml @15:40:55.13 PSV.dial PTV @15:40:55.86 PBV.dial PSV @15:40:55.95 PS.seek 0ml @15:41:22.34 PSV.dial PBV @15:41:22.82 PS.seek 2.5ml @15:41:49.16 PSV.dial :waste1 @15:41:49.90 PS.seek 0ml @15:42:16.26
Loading FlushPuck into Collection Clamp from Processing Clamp @15:42:16.87 Elbow.seek PC @15:42:19.61 Elbow.reconfigure :loose @15:42:19.72 Forearm.seek PC @15:42:20.68 Hand.close @15:42:21.27 PC.seek :open @15:42:26.00 Forearm.seek :retract @15:42:26.95 Elbow.reconfigure :tight @15:42:27.00 Elbow.seek CC @15:42:30.50 Elbow.reconfigure :loose @15:42:30.60 Forearm.reconfigure :tight @15:42:30.65 Forearm.seek CC @15:42:31.98 CC.seek :closed @15:42:36.51 Collection Clamp onSpring/offSpring for 13/27 samples, on/off torque 2.31 @15:42:36.57 Hand.open @15:42:37.20 Forearm.reconfigure :loose @15:42:37.25 Forearm.seek :retract @15:42:38.18 Flushing the Collection Valve::Manifold @15:42:38.31 CSV.dial CSR @15:42:38.75 Pulling 5ml of CSR.flush @15:42:38.76 CSR.select :flush @15:42:38.88 CS.seek 5ml @15:42:52.57 delay 5 seconds @15:42:57.58 CSR.select :air @15:42:57.75 CS.reconfigure :fast @15:42:57.80 CS.seek 7.5ml @15:43:04.53 CSV.dial :waste @15:43:05.82 CS.reconfigure :default @15:43:05.87 CS.seek 0ml @15:43:26.07 Flushing lower manifold's waste1 port @15:43:26.19 CSV.dial :flush @15:43:26.63 CS.reconfigure :fast @15:43:27.15 CS.seek 5ml @15:43:39.97 delay 5 seconds @15:43:44.99 CSV.dial :air @15:43:45.63 CS.seek 7ml @15:43:51.29 CSV.dial CTV @15:43:51.31
CTV.dial CSV @15:43:52.69 CBV.dial :waste1 @15:43:53.45 CS.seek 0ml @15:44:10.29 Flushing lower manifold's waste2 port @15:44:10.41 CSV.dial :flush @15:44:11.15 CS.seek 5ml @15:44:23.97 delay 5 seconds @15:44:28.99 CSV.dial :air @15:44:29.52 CS.seek 7ml @15:44:35.22 CSV.dial CTV @15:44:35.25
CTV.dial CSV @15:44:35.75 CBV.dial :waste2 @15:44:36.79 CS.seek 0ml @15:44:53.75 Flushing lower manifold's waste2b port @15:44:53.88 CSV.dial :flush @15:44:54.62 CS.seek 5ml @15:45:07.66 delay 5 seconds @15:45:12.68 CSV.dial :air @15:45:13.21 CS.seek 7ml @15:45:18.82 CSV.dial CTV @15:45:18.84
CTV.dial CSV @15:45:19.30 CBV.dial :waste2b @15:45:20.32 CS.seek 0ml @15:45:37.16 Disinfecting external Intake with kill @15:45:37.28 CSV.dial CSR @15:45:37.72 Pulling 8ml of CSR.kill @15:45:37.73 CSR.select :kill @15:45:37.85 CS.seek 8ml @15:45:58.03 delay 5 seconds @15:46:03.05 CSR.select :air @15:46:03.17 CS.seek 10ml @15:46:08.81 CSV.dial CBV @15:46:08.84
CBV.dial CSV @15:46:09.94 CTV.dial :intake @15:46:10.67 Intake.select :open @15:46:10.78 CS.seek 2ml @15:46:29.90 Intake.select :close @15:46:30.01 CTV.dial :air @15:46:30.75 CS.reconfigure :default @15:46:30.80 CS.seek 0ml @15:46:36.75 CSV.dial CSR @15:46:38.33 Pulling 5ml of CSR.flush @15:46:38.34 CSR.select :flush @15:46:38.46 CS.seek 5ml @15:46:52.31 delay 5 seconds @15:46:57.33 CSR.select :air @15:46:57.45 CS.seek 10ml @15:47:11.15 CSV.dial CBV @15:47:12.15 CS.seek 0ml @15:47:38.59 Garaging FlushPuck in Collection Clamp @15:47:38.66 Elbow.reconfigure :tight @15:47:38.71 Elbow.seek CC @15:47:39.02 Elbow.reconfigure :loose @15:47:39.12 Forearm.seek CC @15:47:40.13 Hand.close @15:47:40.76 CC.seek :open @15:47:45.43 Forearm.seek :retract @15:47:46.43 Elbow.reconfigure :tight @15:47:46.48 Elbow.seek :garage @15:47:49.33 Elbow.reconfigure :loose @15:47:49.43 Forearm.reconfigure :tight @15:47:49.48 Forearm.seek :garage @15:47:50.48 Hand.open @15:47:51.20 Forearm.reconfigure :loose @15:47:51.24 Forearm.seek :retract @15:47:52.26 Completed [initialPurge; da [50, 50] #da and wsr are run simultanelously] begun 13:57:18 @15:47:52.31 Asleep after completing DA_WCR_011024 assays @15:47:52.33 GoodNews.email "{1 => 4,\n 2 => 0,\n 3 => 0,\n 4 => 0,\n 5 => 0,\n 6 => 0,\n 7 => 0}\nCTD data unavailable\nCan@15:46:32, 25% flow, 23.2C, 12% humidity, 14.8psia, 13.749V, 0.514A, 65.231Ah, 7.07W\nSampled 50.0ml",Subject: "Asleep after completing DA_WCR_011024 assays" @15:47:56.37 upsync + ESPwhich DA_WCR_011024.rb + fn=DA_WCR_011024.rb + IFS=: + set /home/esp/esp2/mission . + fullPath=/home/esp/esp2/mission/DA_WCR_011024.rb + [ -f /home/esp/esp2/mission/DA_WCR_011024.rb ] + realpath /home/esp/esp2/mission/DA_WCR_011024.rb + return 0 + scriptPath=/home/esp/esp2local/mission/DA_WCR_011024.rb + exec ruby /home/esp/esp2/lib/esp.rb /home/esp/esp2local/mission/DA_WCR_011024.rb @13:55:41.37UTC22-Jan-24
ESP2 v3.42 I2C Gateway v3.20 for core bus @13:55:44.14 Configuration for short puck jake 1/11/21 by brent@mbari.org /home/esp/esp2/type/shallow/jake/configure.rb @13:55:47.97 Polling Can every 10:00 @13:55:47.99 Cleanly exited at 13:55:14UTC22-Jan-24 @13:55:48.04 Loading /home/esp/esp2local/mission/DA_WCR_011024.rb mission @13:55:48.10 GoodNews.email "Starting DA_WCR_011024 w/pucks in tube 2\n{3 => 0,\n 4 => 0,\n 5 => 0,\n 6 => 0,\n 7 => 0}\nCTD data unavailable\nCan@13:55:47, 26% flow, 23.2C, 8% humidity, 15.2psia, 13.793V, 0.375A, 84.435Ah, 5.17W",Subject: "DA_WCR_011024 begun" @13:55:48.14
Preparing to sleep until + 00:00:05 @13:55:48.17 Asleep til + 00:00:05 @13:55:48.19 Continuing at 13:55 @13:55:48.47 Gate.power :core,:OFF @13:55:48.49 Gate.power :raw,:OFF Shutdown @13:55:50.90 GoodNews.email "Then, in tube 2: initialPurge; da [50, 50] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_011024.rb:14\n{1 => 0,\n 2 => 4,\n 3 => 0,\n 4 => 0,\n 5 => 0,\n 6 => 0,\n 7 => 0}\nCTD data unavailable\nCan@13:55:48, 26% flow, 23.2C, 8% humidity, 15.2psia, 13.793V, 0.381A, 84.435Ah, 5.25W",Subject: "Asleep until 13:55" @13:55:52.25 upsync @13:55:53.08
Powering core bus @13:55:54.88 upsync @13:55:58.51
Gate.power :raw,:ON @13:55:59.50 CS.configure :default @13:55:59.55 PS.configure :default @13:55:59.60 SS.configure :default @13:55:59.65 CC.configure :default @13:55:59.70 PC.configure :default @13:55:59.77 Forearm.configure :loose @13:55:59.81 Elbow.configure :loose @13:55:59.88 SC.configure :loose @13:55:59.93 SE.configure :default @13:55:59.98 CH.configure :default @13:56:00.01 PH.configure :default @13:56:00.05 All dwarf controllers running firmware version 3.85 @13:56:00.16 Valve.reset @13:56:00.39 Intake.select :close @13:56:00.41 Exhaust.select :close @13:56:00.46 CTV.dial :air @13:56:00.57 PSV.dial :air @13:56:00.63 CSV.dial :waste1 @13:56:00.76
Forearm.home.seek :retract @13:56:01.13 SSV.dial :spit,avoiding: :exhaust @13:56:01.52 Hand.close @13:56:01.56
Elbow.reconfigure :loose @13:56:01.64 Elbow.jog 1500 @13:56:01.84 PS.home.seek 0ml @13:56:02.01 CS.reconfigure :fast @13:56:02.05 SS.home.seek 0ml @13:56:02.15 CS.home.seek 0ml @13:56:02.24
Elbow.reconfigure :loose @13:56:02.30 Elbow.home.seek :clear @13:56:05.55 SE.home.seek :down @13:56:06.16 SC.reconfigure :tight @13:56:06.20 SC.home.seek :initial @13:56:10.11 PS.stop @13:56:31.37
SC.configure :limp @13:56:31.43 Forearm.seek :retract @13:56:31.50 Elbow.seek :hammer @13:56:32.52 Forearm.reconfigure :tight @13:56:32.57 Forearm.seek retracted @13:56:32.97 PC.home.seek :open @13:56:32.98
CC.home.seek :open @13:56:36.43 Phase [initialPurge; da [50, 50] #da and wsr are run simultanelously] @13:56:36.45 Notice.email "At 13:56:36 began in tube 2: initialPurge; da [50, 50] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_011024.rb:14\n{1 => 0,\n 2 => 4,\n 3 => 0,\n 4 => 0,\n 5 => 0,\n 6 => 0,\n 7 => 0}\nCTD data unavailable\nCan@13:56:36, 26% flow, 23.3C, 8% humidity, 15.2psia, 13.764V, 0.315A, 84.440Ah, 4.33W",Subject: "Started DA_WCR_011024 phase initialPurge; da [50, 50] #da and wsr are run simultanelously" @13:56:36.47
Starting initialPurge @13:56:36.49 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @13:56:36.62 Hand.open @13:56:37.39 Elbow.reconfigure :tight @13:56:37.44 Elbow.seek FlushPuck::Garage @13:56:39.44 Elbow.reconfigure :loose @13:56:39.66 Forearm.reconfigure :loose @13:56:39.75 Forearm.seek :garage @13:56:40.71 Hand.close @13:56:40.94 upsync @13:56:41.39
Forearm.seek :retract @13:56:42.43 Elbow.reconfigure :tight @13:56:42.48 Elbow.seek CC @13:56:45.39 Elbow.reconfigure :loose @13:56:45.49 Forearm.reconfigure :tight @13:56:45.54 Forearm.seek CC @13:56:46.80 CC.seek :closed @13:56:51.43 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.39 @13:56:51.50 Hand.open @13:56:52.21 Forearm.reconfigure :loose @13:56:52.26 Forearm.seek :retract @13:56:53.25 Flushing the Collection Valve::Manifold @13:56:53.38 CSV.dial CSR @13:56:54.64 Pulling 5ml of CSR.flush @13:56:54.65 CSR.select :flush @13:56:54.77 CS.reconfigure :default @13:56:54.81 CS.seek 5ml @13:57:08.62 delay 5 seconds @13:57:13.64 CSR.select :air @13:57:14.22 CS.reconfigure :fast @13:57:14.27 CS.seek 7.5ml @13:57:21.09 CSV.dial :waste @13:57:22.36 CS.reconfigure :default @13:57:22.41 CS.seek 0ml @13:57:42.53 Purging external Intake @13:57:42.66 CSV.dial :flush @13:57:43.10 CS.reconfigure :fast @13:57:43.15 CS.seek 10ml @13:58:07.65 delay 5 seconds @13:58:12.68 CSV.dial CBV @13:58:12.71
CBV.dial CSV @13:58:13.45 CTV.dial :intake @13:58:14.23 Intake.select :open @13:58:14.34 CS.seek 0ml @13:58:37.90 Intake.select :close @13:58:38.00 CTV.dial :air @13:58:38.78 Flushing upper manifold @13:58:38.90 CSV.dial :flush @13:58:39.64 CS.seek 5ml @13:58:52.34 delay 5 seconds @13:58:57.36 CSV.dial :air @13:58:57.87 CS.seek 7ml @13:59:03.53 CSV.dial CBV @13:59:03.57
CBV.dial CSV @13:59:04.57 CTV.dial :air @13:59:04.82 CS.seek 0ml @13:59:21.43 delay 7 seconds @13:59:28.46 CS.seek 3ml @13:59:36.65 CSV.dial :waste @13:59:37.11 CS.seek 0ml @13:59:44.92 CSV.dial :flush @13:59:45.36 CS.seek 3ml @13:59:53.44 delay 5 seconds @13:59:58.45 CSV.dial :air @13:59:58.97 CS.seek 5ml @14:00:04.68 CSV.dial :air @14:00:04.77 CS.seek 0ml @14:00:16.96 delay 7 seconds @14:00:23.99 CS.seek 3ml @14:00:32.24 CSV.dial :waste @14:00:32.98 CS.seek 0ml @14:00:40.71 Garaging FlushPuck in Collection Clamp @14:00:40.78 Elbow.reconfigure :tight @14:00:40.83 Elbow.seek CC @14:00:41.09 Elbow.reconfigure :loose @14:00:41.19 Forearm.seek CC @14:00:42.23 Hand.close @14:00:42.89 CC.seek :open @14:00:47.95 Forearm.seek :retract @14:00:48.92 Elbow.reconfigure :tight @14:00:48.97 Elbow.seek :garage @14:00:51.90 Elbow.reconfigure :loose @14:00:52.00 Forearm.reconfigure :tight @14:00:52.05 Forearm.seek :garage @14:00:53.07 Hand.open @14:00:53.70 Forearm.reconfigure :loose @14:00:53.75 Forearm.seek :retract @14:00:54.73 da sampling at most 50ml, wcr at most 50ml @14:00:54.77 DAfans.email "Started da in tube 2 at 14:00:54UTC22-Jan-24\n{1 => 0,\n 2 => 4,\n 3 => 0,\n 4 => 0,\n 5 => 0,\n 6 => 0,\n 7 => 0}\nCTD data unavailable\nCan@13:56:36, 26% flow, 23.3C, 8% humidity, 15.2psia, 13.764V, 0.315A, 84.440Ah, 4.33W",Subject: "da sampling at most 50ml, wcr at most 50ml" @14:00:54.81
Loading sh1 puck from tube 2 into Collection Clamp @14:00:55.07 SC.reconfigure :tight @14:00:55.12 SC.seek 2 @14:00:58.90 upsync @14:01:01.83
SC.configure :limp @14:01:02.06 Elbow.reconfigure :tight @14:01:02.11 Elbow.seek 2 @14:01:04.15 Forearm.reconfigure :tight @14:01:04.20 Forearm.seek 2 + 450 counts @14:01:05.23 SE.reconfigure :pick @14:01:05.29 SE.seek :beamBreak @14:01:08.20 SE.configure :default @14:01:08.37 Elbow.seek 2 @14:01:08.64 Forearm.seek 2 + 300 counts @14:01:08.86 Forearm.reconfigure :loose @14:01:08.90 Hand.close @14:01:09.53 SE.seek :down @14:01:09.74
Forearm.seek :retract @14:01:10.71 Elbow.seek CC @14:01:12.16 SE.stop @14:01:12.41
Elbow.reconfigure :loose @14:01:12.52 Forearm.reconfigure :tight @14:01:12.57 Forearm.seek CC @14:01:13.77 CC.seek :closed @14:01:18.31 Collection Clamp onSpring/offSpring for 12/26 samples, on/off torque 2.18 @14:01:18.32 Hand.open @14:01:18.95 Forearm.reconfigure :loose @14:01:19.00 Forearm.seek :retract @14:01:19.94 collectSample 50ml w/bubble point of 24psi @14:01:19.96 Priming 7ml @14:01:19.97 Intake.select :close @14:01:19.98 Exhaust.select :close @14:01:19.99 SS.hold @14:01:20.20 Calibrating pressure and temperature sensors @14:01:20.23 CTV.dial :air @14:01:20.31 SSV.dial :spit,avoiding: :exhaust @14:01:20.51 SS.reconfigure :fast @14:01:20.56 SS.seek 5ml @14:01:23.42 SSV.dial CBV @14:01:23.45
CBV.dial SSV @14:01:24.78 delay 3.8 seconds @14:01:28.70 Sensor limits: Intake max 121.1psia; Outlet max 115.9psia @14:01:28.71 SSV.dial :spit @14:01:30.01 SS.seek 0ml @14:01:32.97 SSV.dial CBV @14:01:34.28 CTV.dial :intake @14:01:35.01 SSV.dial CBV @14:01:35.04
CBV.dial SSV @14:01:35.12 Allowing 40s for 7.0ml of remaining 7.0ml @14:01:35.13 Intake.select :open @14:01:37.30 Intake @15.7psia, vacuum from 10.0 to 13.0psi @14:01:37.31 SS.reconfigure :inhaling 2.20ml/s @14:01:37.36 SS.seek 7ml @14:01:44.41 Stroke completed -- waiting for pressure to equalize ... @14:01:44.42 SS.reconfigure :exhale @14:01:44.47 SS.hold @14:01:46.43 Intake.select :close @14:01:46.66 SSV.dial :exhaust @14:01:47.41 Exhaust.select :open @14:01:47.53 SS.seek 0ml @14:01:51.43 Exhaust.select :close @14:01:51.54 Sampling 50.0ml @14:01:51.55 Intake.select :close @14:01:51.56 Exhaust.select :close @14:01:51.57 SS.reconfigure :default @14:01:51.62 SS.hold @14:01:51.83 CTV.dial :intake @14:01:51.90 SSV.dial CBV @14:01:51.93
CBV.dial SSV @14:01:52.69 Allowing 130s for 25.0ml of remaining 50.0ml @14:01:52.70 Intake.select :open @14:01:54.87 Intake @15.9psia, vacuum from 10.0 to 13.0psi @14:01:54.89 SS.reconfigure :inhaling 2.20ml/s @14:01:54.94 SS.seek 25ml @14:02:08.72 Vacuum too high -- waiting up to 40 seconds @14:02:08.73 SS.reconfigure :exhale @14:02:08.78 SS.hold @14:02:09.49 Intake @12.2psia, vacuum from 6.7 to 9.7psi @14:02:09.51 SS.reconfigure :inhaling 1.76ml/s @14:02:09.56 SS.seek 25ml @14:02:11.60 Stroke completed -- waiting for pressure to equalize ... @14:02:11.61 SS.reconfigure :exhale @14:02:11.65 SS.hold @14:02:15.19 Intake.select :close @14:02:15.34 SSV.dial :exhaust @14:02:16.07 Exhaust.select :open @14:02:16.18 SS.seek 0ml @14:02:24.94 Exhaust.select :close @14:02:25.06 SSV.dial CBV @14:02:25.08
CBV.dial SSV @14:02:25.83 Allowing 130s for 25.0ml of remaining 25.0ml @14:02:25.84 Intake.select :open @14:02:28.01 Intake @16.0psia, vacuum from 10.0 to 13.0psi @14:02:28.03 SS.reconfigure :inhaling 1.76ml/s @14:02:28.08 SS.seek 25ml @14:02:42.81 Vacuum too high -- waiting up to 40 seconds @14:02:42.82 SS.reconfigure :exhale @14:02:42.87 SS.hold @14:02:43.47 Intake @13.3psia, vacuum from 7.8 to 10.8psi @14:02:43.49 SS.reconfigure :inhaling 1.41ml/s @14:02:43.96 SS.seek 25ml @14:02:47.77 Stroke completed -- waiting for pressure to equalize ... @14:02:47.79 SS.reconfigure :exhale @14:02:47.83 SS.hold @14:02:51.98 Intake.select :close @14:02:52.14 SSV.dial :exhaust @14:02:52.90 Exhaust.select :open @14:02:53.02 SS.seek 0ml @14:03:01.83 Exhaust.select :close @14:03:01.94 Sampled 50.0ml @14:03:01.96 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @14:03:01.97 Intake.select :close @14:03:02.03 CSV.dial :waste @14:03:02.17 SSV.dial CBV,avoiding: :exhaust @14:03:02.20
CBV.dial SSV @14:03:02.33 CS.seek 0ml @14:03:02.40 CSV.dial :air @14:03:03.12 CS.seek 10ml @14:03:04.10
CTV.dial :air @14:03:04.88 delay 15 seconds @14:03:19.90 SSV.dial :spit,avoiding: :exhaust @14:03:21.21 CTV.dial CSV,avoiding: :intake @14:03:21.25
SS.seek 0ml @14:03:21.38 SSV.dial CBV,avoiding: :exhaust @14:03:27.81 SSV.dial between CBV and plug @14:03:28.29 CBV.dial between SSV and waste2 @14:03:28.68 CTV.dial CSV,avoiding: :air @14:03:28.71
CSV.dial CTV @14:03:29.20 CS.reconfigure :pressurize @14:03:29.25 CS.seek 0ml @14:03:47.84 BubblePoint: Intake @24.2psia, Outlet @0.7psia @14:03:47.85 CBV.dial SSV @14:03:48.18 SS.reconfigure :default @14:03:48.23 SS.seek 10ml @14:03:53.91 delay 15 seconds @14:04:09.04 Evacuated: Intake @23.9psia, Outlet @23.8psia @14:04:09.05 SSV.dial :spit,avoiding: :exhaust @14:04:10.52 SS.reconfigure :exhale @14:04:10.57 SS.seek 0ml @14:04:15.59 SS.reconfigure :fast @14:04:15.64 SS.seek 5ml @14:04:18.45 SSV.dial CBV,avoiding: :exhaust @14:04:18.47
CBV.dial SSV @14:04:19.82 CTV.dial :air,avoiding: :intake @14:04:21.14 CSV.dial CTV @14:04:21.17
SS.seek 10ml @14:04:22.37 CSV.dial :air @14:04:22.81 CS.reconfigure :fast @14:04:22.86 CS.seek 0ml @14:04:23.69
SSV.dial :spit,avoiding: :exhaust @14:04:25.01 SS.reconfigure :exhale @14:04:25.06 SS.seek 0ml @14:04:30.11 SS.reconfigure :fast @14:04:30.15 SS.seek 5ml @14:04:30.36 CS.stop @14:04:33.30 SSV.dial CBV,avoiding: :exhaust @14:04:33.33
CBV.dial SSV @14:04:35.66 SSV.dial :spit,avoiding: :exhaust @14:04:36.98 SS.reconfigure :default @14:04:37.02 SS.seek 0ml @14:04:40.10 SS.stop @14:04:40.15 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @14:04:40.16 Intake.select :close @14:04:40.22 CSV.dial :waste @14:04:40.36 SSV.dial CBV,avoiding: :exhaust @14:04:40.39
CBV.dial SSV @14:04:41.01 CS.seek 0ml @14:04:41.08 CSV.dial :air @14:04:41.72
CTV.dial :air @14:04:41.90 delay 15 seconds @14:04:41.94 CS.seek 10ml @14:04:56.92
SSV.dial :spit,avoiding: :exhaust @14:04:58.24 CTV.dial CSV,avoiding: :intake @14:04:58.28
SS.reconfigure :exhale @14:04:58.38 SS.seek 0ml @14:04:58.80 SSV.dial CBV,avoiding: :exhaust @14:05:06.40 SSV.dial between CBV and plug @14:05:06.88 CBV.dial between SSV and waste2 @14:05:07.27 CSV.dial between air and CTV @14:05:07.59 CS.seek 8.222ml @14:05:12.40 CTV.dial CSV,avoiding: :air @14:05:12.43
CSV.dial CTV @14:05:12.81 CS.reconfigure :pressurize @14:05:12.86 CS.seek 0ml @14:05:30.91 BubblePoint: Intake @24.2psia, Outlet @0.6psia @14:05:30.92 CBV.dial SSV @14:05:31.24 SS.reconfigure :default @14:05:31.29 SS.seek 10ml @14:05:36.94 delay 15 seconds @14:05:52.07 Evacuated: Intake @23.8psia, Outlet @15.1psia @14:05:52.08 SSV.dial :spit,avoiding: :exhaust @14:05:53.12 SS.reconfigure :exhale @14:05:53.17 SS.seek 0ml @14:05:58.21 SS.reconfigure :fast @14:05:58.26 SS.seek 5ml @14:06:01.08 SSV.dial CBV,avoiding: :exhaust @14:06:01.11
CBV.dial SSV @14:06:02.46 CTV.dial :air,avoiding: :intake @14:06:03.76 CSV.dial CTV @14:06:03.81
SS.seek 10ml @14:06:04.98 CSV.dial :air @14:06:05.41 CS.reconfigure :fast @14:06:05.47 CS.seek 0ml @14:06:06.31
SSV.dial :spit,avoiding: :exhaust @14:06:07.63 SS.reconfigure :exhale @14:06:07.68 SS.seek 0ml @14:06:09.50 CS.stop @14:06:13.01
SS.reconfigure :fast @14:06:13.06 SS.seek 5ml @14:06:15.90 SSV.dial CBV,avoiding: :exhaust @14:06:15.92
CBV.dial SSV @14:06:18.25 SSV.dial :spit,avoiding: :exhaust @14:06:19.57 SS.reconfigure :default @14:06:19.62 SS.seek 0ml @14:06:22.94 SS.stop @14:06:22.97 Lysing: Adding 2.0ml AqMeOH @14:06:22.98 CBV.dial between CSV and waste1,avoiding: :DBV @14:06:24.44 CTV.dial CSV @14:06:25.74 CSV.dial CSR @14:06:26.53 Pulling 1ml of CSR.AqMeOH @14:06:26.54 CSR.select :AqMeOH @14:06:26.66 CS.reconfigure :default @14:06:26.70 CS.seek 1ml @14:06:30.08 delay 5 seconds @14:06:35.10 CSR.select :air @14:06:35.27 CS.seek 6ml @14:06:48.86 delay 2 seconds @14:06:50.88 CSV.dial :waste2 @14:06:51.32 CS.reconfigure :fast @14:06:51.37 CS.seek 0ml @14:07:05.74 CSV.dial CSR @14:07:06.19 Pulling 2ml of CSR.AqMeOH @14:07:06.20 CSR.select :AqMeOH @14:07:06.32 CS.reconfigure :default @14:07:06.36 CS.seek 2ml @14:07:12.30 delay 5 seconds @14:07:17.32 CSR.select :air @14:07:17.49 CS.seek 5ml @14:07:25.96 delay 2 seconds @14:07:27.97 CSV.dial CTV @14:07:28.41 CS.seek 1ml @14:07:39.85 Before lysing Collection Puck Heater at 22.4C/25.4psia @14:07:39.87 Heating Puck.from 2, :sh1 in Collection Clamp to 70C for 10:00 @14:07:39.88 CH.seek 70C,8:00 @14:09:29.53 CH.hold 10:00,70C @14:09:29.74 Begun lysing Collection Puck Heater at 68C/30.9psia @14:19:29.74 Ended lysing Collection Puck Heater at 70.1C/29.6psia @14:19:29.76 CS.seek 0ml @14:19:33.14 CTV.dial between spare and air,avoiding: :intake @14:19:34.15 CSV.dial :air @14:19:34.59 CS.seek 5ml @14:19:48.41 CSV.dial CTV @14:19:48.87 delay 2 seconds @14:19:50.88 CSV.dial :air @14:19:51.33 delay 2 seconds @14:19:53.35 CSV.dial CTV @14:19:53.80 delay 2 seconds @14:19:55.82 CSV.dial :air @14:19:56.34 delay 2 seconds @14:19:58.36 CS.seek 0ml @14:20:12.03 Puck @58.8psia after final lysis pressurization @14:20:12.04 Recovering Lysate into Collection syringe @14:20:12.05 CSV.dial CBV @14:20:13.05 CBV.dial CSV @14:20:13.45 CS.seek 5ml @14:20:27.22 delay 5 seconds @14:20:32.24 CTV.dial :air @14:20:32.79 CS.seek 7ml @14:20:38.66 delay 5 seconds @14:20:43.68 CSV.dial :air2 @14:20:44.12 CS.seek 10ml @14:20:52.57 CSV.dial between air and CTV,avoiding: CBV @14:20:53.83 Lysing Complete @14:20:53.90 Storing sh1 puck in Collection Clamp to tube 1 @14:20:54.08 SC.reconfigure :tight @14:20:54.13 SC.seek 1 @14:20:57.70 SC.configure :limp @14:20:57.86 SE.reconfigure :place @14:20:57.95
Elbow.reconfigure :tight @14:20:57.99 SE.seek :beamBreak @14:20:58.01
Elbow.seek CC @14:20:58.49 Elbow.reconfigure :loose @14:20:58.59 Forearm.seek CC @14:20:59.59 Hand.close @14:21:00.25 CC.seek :open @14:21:00.87 SE.configure :default @14:21:00.92 SE.seek :place @14:21:04.99
Forearm.seek :retract @14:21:06.00 Elbow.reconfigure :tight @14:21:06.05 Elbow.seek 1 @14:21:08.02 Elbow.reconfigure :limp @14:21:08.08 Forearm.seek 1 @14:21:09.04 Elbow.hold @14:21:09.11 Elbow.reconfigure :tight @14:21:09.16 Hand.open @14:21:09.87 Forearm.force -20 @14:21:10.36 Forearm.seek 1 + 311 counts @14:21:10.67 Forearm.reconfigure :tight @14:21:10.72 Forearm.seek 1 + 450 counts @14:21:10.98 SE.seek :down @14:21:12.13
Forearm.reconfigure :loose @14:21:12.18 Forearm.seek :retract @14:21:13.66 SE.stop @14:21:13.70
Tranferring DA @14:21:13.71 Beginning samplePassC @14:21:13.73 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:21:13.92 Elbow.seek FlushPuck::Garage @14:21:15.74 Elbow.reconfigure :loose @14:21:15.84 Forearm.seek :garage @14:21:16.79 Hand.close @14:21:17.49 Forearm.seek :retract @14:21:18.51 Elbow.reconfigure :tight @14:21:18.56 Elbow.seek CC @14:21:21.45 Elbow.reconfigure :loose @14:21:21.55 Forearm.reconfigure :tight @14:21:21.60 Forearm.seek CC @14:21:22.84 CC.seek :closed @14:21:27.36 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.35 @14:21:27.42 Hand.open @14:21:28.06 Forearm.reconfigure :loose @14:21:28.12 Forearm.seek :retract @14:21:29.54 PSV.dial PSR @14:21:30.94 Pulling 1.5ml of PSR.flush @14:21:30.95 PSR.select :flush @14:21:31.07 PS.seek 1.5ml @14:21:47.30 delay 5 seconds @14:21:52.32 PSV.dial :waste1 @14:21:52.80 PS.seek 0ml @14:22:08.98 PSV.dial PSR @14:22:09.56 PSR.select :air @14:22:09.68 PS.seek 2.5ml @14:22:36.24 PRV.dial CBV,avoiding: :ab2 @14:22:36.28
CBV.dial PRV @14:22:38.00 PSV.dial :mixloop @14:22:39.05 CTV.dial :air,avoiding: :intake @14:22:39.28 PTV.dial :PRVmixing @14:22:40.60 PS.seek 0ml @14:23:06.95 PSV.dial :air @14:23:07.54 PS.seek 2.5ml @14:23:33.92 PSV.dial :mixloop @14:23:34.38 PS.seek 0ml @14:24:00.70 PSV.dial :air @14:24:01.18 PS.seek 2.5ml @14:24:27.51 PSV.dial :mixloop @14:24:27.99 PS.seek 0ml @14:24:54.39 Garaging FlushPuck in Collection Clamp @14:24:54.46 Elbow.reconfigure :tight @14:24:54.51 Elbow.seek CC @14:24:54.77 Elbow.reconfigure :loose @14:24:54.87 Forearm.seek CC @14:24:55.91 Hand.close @14:24:56.50 CC.seek :open @14:25:01.12 Forearm.seek :retract @14:25:02.13 Elbow.reconfigure :tight @14:25:02.18 Elbow.seek :garage @14:25:05.05 Elbow.reconfigure :loose @14:25:05.15 Forearm.reconfigure :tight @14:25:05.20 Forearm.seek :garage @14:25:06.30 Hand.open @14:25:06.93 Forearm.reconfigure :loose @14:25:07.35 Forearm.seek :retract @14:25:08.41 PSV.dial :mixloop @14:25:08.50 PTV.dial :PRVmixing @14:25:08.58 PRV.dial CBV @14:25:08.62
CBV.dial PRV @14:25:08.78 CTV.dial :air,avoiding: :intake @14:25:08.80 Loading lyfil puck from tube 2 into Collection Clamp @14:25:09.04 SC.reconfigure :tight @14:25:09.09 SC.seek 2 @14:25:12.64 SC.configure :limp @14:25:12.86 Elbow.reconfigure :tight @14:25:12.91 Elbow.seek 2 @14:25:14.80 Forearm.reconfigure :tight @14:25:14.85 Forearm.seek 2 + 450 counts @14:25:15.88 SE.reconfigure :pick @14:25:15.94 SE.seek :beamBreak @14:25:18.95 SE.configure :default @14:25:19.11 Elbow.seek 2 @14:25:19.30 Forearm.seek 2 + 300 counts @14:25:19.54 Forearm.reconfigure :loose @14:25:19.59 Hand.close @14:25:20.26 SE.seek :down @14:25:20.46
Forearm.seek :retract @14:25:21.48 Elbow.seek CC @14:25:22.95 SE.stop @14:25:23.11
Elbow.reconfigure :loose @14:25:23.22 Forearm.reconfigure :tight @14:25:23.27 Forearm.seek CC @14:25:24.96 CC.seek :closed @14:25:29.48 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.13 @14:25:29.49 Hand.open @14:25:30.18 Forearm.reconfigure :loose @14:25:30.23 Forearm.seek :retract @14:25:31.21 CTV.dial CSV,avoiding: :intake @14:25:32.53 CSV.dial CTV @14:25:32.83 PS.seek 2.5ml @14:25:59.29 CS.seek 4.5ml @14:26:14.25 delay 8 seconds @14:26:22.27 PSV.dial :air @14:26:22.73 delay 2 seconds @14:26:24.75 PSV.dial :waste2 @14:26:25.47 PS.seek 0ml @14:26:51.85 CBV.dial between PRV and waste1 @14:26:52.19 CSV.dial :air @14:26:52.64 CS.seek 10ml @14:27:07.59 CSV.dial CTV @14:27:08.05 CS.seek 0ml @14:27:34.62 CBV.dial :waste1 @14:27:34.94 delay 5 seconds @14:27:39.96 CTV.dial :air,avoiding: :intake @14:27:41.35 Storing lyfil puck in Collection Clamp to tube 1 @14:27:41.53 SC.reconfigure :tight @14:27:41.58 SC.seek 1 @14:27:45.13 SC.configure :limp @14:27:45.36 Elbow.reconfigure :tight @14:27:45.41 Elbow.seek 1 @14:27:47.65 Forearm.reconfigure :tight @14:27:47.70 Forearm.seek 1 + 450 counts @14:27:48.71 SE.reconfigure :pick @14:27:48.77 SE.seek :beamBreak @14:27:51.79 SE.configure :default @14:27:51.95 Elbow.reconfigure :limp @14:27:52.00 Forearm.reconfigure :loose @14:27:52.05 Forearm.seek 1 @14:27:52.27 Elbow.hold @14:27:52.34 Elbow.reconfigure :tight @14:27:52.46 SE.seek 0.17 Pucks below Top Plate @14:27:52.85 Forearm.force -20 @14:27:52.90 Forearm.seek 1 + 314 counts @14:27:53.21 Forearm.reconfigure :tight @14:27:53.26 Forearm.seek 1 + 450 counts @14:27:53.45 SE.seek 1.17 Pucks below Top Plate @14:27:53.95 Forearm.reconfigure :loose @14:27:54.00 Forearm.seek :retract @14:27:54.96 Elbow.seek CC @14:27:56.59 Elbow.reconfigure :loose @14:27:56.69 Forearm.seek CC @14:27:57.71 Hand.close @14:27:58.37 CC.seek :open @14:28:03.15 Forearm.seek :retract @14:28:04.13 Elbow.reconfigure :tight @14:28:04.18 Elbow.seek 1 @14:28:05.95 Elbow.reconfigure :limp @14:28:06.45 Forearm.seek 1 @14:28:07.47 Elbow.hold @14:28:07.54 Elbow.reconfigure :tight @14:28:07.59 Hand.open @14:28:08.37 Forearm.force -20 @14:28:08.42 Forearm.seek 1 + 313 counts @14:28:08.72 Forearm.reconfigure :tight @14:28:08.77 Forearm.seek 1 + 450 counts @14:28:09.04 SE.seek :down @14:28:10.18
Forearm.reconfigure :loose @14:28:10.23 Forearm.seek :retract @14:28:11.68 SE.stop @14:28:11.73
PSV.dial :air @14:28:12.47 PS.seek 2.5ml @14:28:38.79 delay 1 second @14:28:39.80 PSV.dial :mixloop @14:28:40.24 delay 1 second @14:28:41.26 PSV.dial :air @14:28:41.84 delay 1 second @14:28:42.85 PSV.dial :mixloop @14:28:43.31 delay 1 second @14:28:44.32 PSV.dial :air @14:28:44.79 delay 1 second @14:28:45.81 PS.seek 0ml @14:29:12.16 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @14:29:12.36 Elbow.seek FlushPuck::Garage @14:29:14.36 Elbow.reconfigure :loose @14:29:14.46 Forearm.seek :garage @14:29:15.50 Hand.close @14:29:16.23 Forearm.seek :retract @14:29:17.28 Elbow.reconfigure :tight @14:29:17.33 Elbow.seek PC @14:29:18.61 Elbow.reconfigure :loose @14:29:19.14 Forearm.reconfigure :tight @14:29:19.19 Forearm.seek PC @14:29:20.44 PC.seek :closed @14:29:25.08 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.59 @14:29:25.15 Hand.open @14:29:25.80 Forearm.reconfigure :loose @14:29:25.85 Forearm.seek :retract @14:29:26.80 PRV.dial :air @14:29:27.27 PSV.dial :mixloop @14:29:27.81 PS.seek 0.3ml @14:29:31.88 PSV.dial PSR @14:29:33.04 Pulling 0.4ml of PSR.air @14:29:33.05 PSR.select :air @14:29:33.07 PS.seek 0.7ml @14:29:37.98 delay 3 seconds @14:29:40.99 PSR.select :air @14:29:41.02 PS.seek 2.5ml @14:30:00.22 delay 3 seconds @14:30:03.24 PSV.dial :mixloop @14:30:04.26 PTV.dial :mixing @14:30:04.99 PBV.dial :waste2 @14:30:05.92 PS.seek 0ml @14:30:32.29 Garaging FlushPuck in Processing Clamp @14:30:32.36 Elbow.reconfigure :tight @14:30:32.41 Elbow.seek PC @14:30:32.55 Elbow.reconfigure :loose @14:30:32.65 Forearm.seek PC @14:30:33.61 Hand.close @14:30:34.24 PC.seek :open @14:30:39.00 Forearm.seek :retract @14:30:40.41 Elbow.reconfigure :tight @14:30:40.46 Elbow.seek :garage @14:30:41.80 Elbow.reconfigure :loose @14:30:41.90 Forearm.reconfigure :tight @14:30:41.95 Forearm.seek :garage @14:30:42.91 Hand.open @14:30:43.55 Forearm.reconfigure :loose @14:30:43.60 Forearm.seek :retract @14:30:44.57 Loading da puck from tube 2 into Processing Clamp @14:30:44.81 SC.reconfigure :tight @14:30:44.86 SC.seek 2 @14:30:48.43 SC.configure :limp @14:30:48.66 Elbow.reconfigure :tight @14:30:48.70 Elbow.seek 2 @14:30:50.55 Forearm.reconfigure :tight @14:30:50.60 Forearm.seek 2 + 450 counts @14:30:51.60 SE.reconfigure :pick @14:30:51.66 SE.seek :beamBreak @14:30:54.70 SE.configure :default @14:30:54.86 Elbow.seek 2 @14:30:55.05 Forearm.seek 2 + 300 counts @14:30:55.43 Forearm.reconfigure :loose @14:30:55.48 Hand.close @14:30:56.13 SE.seek :down @14:30:56.32
Forearm.seek :retract @14:30:57.33 Elbow.seek PC @14:30:58.82 SE.stop @14:30:59.85
Elbow.reconfigure :loose @14:30:59.96 Forearm.reconfigure :tight @14:31:00.01 Forearm.seek PC @14:31:01.37 PC.seek :closed @14:31:06.04 Processing Clamp onSpring/offSpring for 16/26 samples, on/off torque 1.63 @14:31:06.05 Hand.open @14:31:06.68 Forearm.reconfigure :loose @14:31:06.72 Forearm.seek :retract @14:31:07.76 Heating Puck.from 2, :da in Processing Clamp to 28C for 2:00:00 @14:31:07.78 PH.seek 28C,5:00 @14:31:34.26 PH.hold 2:00:00,28C @14:31:34.30 delay 1:30 @14:33:04.34 PSR.select :air @14:33:04.35 PSV.dial PSR @14:33:05.38 Pulling 0.5ml of PSR.wash @14:33:05.39 PSR.select :wash @14:33:05.51 PS.seek 0.5ml @14:33:11.38 delay 5 seconds @14:33:16.40 PSR.select :air @14:33:16.52 PS.seek 2ml @14:33:33.20 PTV.dial :puck @14:33:33.94 PSV.dial PTV @14:33:34.69 PBV.dial :waste1 @14:33:36.07 PS.seek 1.5ml @14:33:42.06 delay 3 seconds @14:33:45.08 PBV.dial between PSV and waste1 @14:33:45.45 PS.seek 0ml @14:34:01.63 delay 5:00 @14:39:01.65 recoverPSRreagent08 @14:39:01.66 PTV.dial :puck @14:39:01.73 PBV.dial :waste1 @14:39:02.09 delay 30 seconds @14:39:32.12 PBV.dial between PSV and waste1 @14:39:32.48 PSR.select :air @14:39:32.50 PSV.dial PSR @14:39:33.25 PS.reconfigure :fast @14:39:33.30 PS.seek 2.5ml @14:39:57.06 PSV.dial PTV @14:39:57.93 delay 5 seconds @14:40:02.95 PSV.dial PSR @14:40:03.68 delay 5 seconds @14:40:08.70 PSV.dial PTV @14:40:09.45 delay 5 seconds @14:40:14.47 PSV.dial :air @14:40:15.36 PS.seek 0ml @14:40:38.56 PTV.dial :PRVmixing,via: PRV @14:40:39.85 PSV.dial :mixloop @14:40:40.31 PRV.dial :ab1 @14:40:41.05 PS.reconfigure :slow @14:40:41.09 PS.seek 0.15ml @14:40:44.84 delay 2 seconds @14:40:46.86 CBV.dial PRV @14:40:46.88
PRV.dial CBV @14:40:47.47 PS.seek 0.34ml @14:40:51.87 delay 2 seconds @14:40:53.89 CBV.dial between PRV and PSV @14:40:54.20 PRV.dial :air @14:40:54.66 PS.seek 0.9ml @14:41:06.62 PRV.dial :waste2 @14:41:07.36 PS.seek 0.5ml @14:41:16.02 addPMLReagent08 @14:41:16.03 PSV.dial :air @14:41:16.50 PS.reconfigure :default @14:41:16.55 PS.seek 2ml @14:41:32.68 PSV.dial :PML @14:41:33.15 PTV.dial :mixing @14:41:33.90 PBV.dial :waste1 @14:41:34.34 PS.seek 1.85ml @14:41:37.17 PBV.dial between PSV and waste1 @14:41:37.55 PS.seek 0ml @14:41:57.27 delay 2 seconds @14:41:59.29 Beginning samplePassC @14:41:59.31 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:41:59.50 Elbow.reconfigure :tight @14:41:59.55 Elbow.seek FlushPuck::Garage @14:42:00.93 Elbow.reconfigure :loose @14:42:01.03 Forearm.seek :garage @14:42:02.02 Hand.close @14:42:02.72 Forearm.seek :retract @14:42:03.71 Elbow.reconfigure :tight @14:42:03.76 Elbow.seek CC @14:42:06.64 Elbow.reconfigure :loose @14:42:06.74 Forearm.reconfigure :tight @14:42:06.79 Forearm.seek CC @14:42:08.04 CC.seek :closed @14:42:12.56 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.30 @14:42:12.62 Hand.open @14:42:13.29 Forearm.reconfigure :loose @14:42:13.34 Forearm.seek :retract @14:42:14.32 PSV.dial PSR @14:42:15.37 Pulling 1.5ml of PSR.flush @14:42:15.38 PSR.select :flush @14:42:15.50 PS.seek 1.5ml @14:42:31.65 delay 5 seconds @14:42:36.67 PSV.dial :waste1 @14:42:37.14 PS.seek 0ml @14:42:53.68 PSV.dial PSR @14:42:54.15 PSR.select :air @14:42:54.27 PS.seek 2.5ml @14:43:20.62 PRV.dial CBV,avoiding: :ab2 @14:43:20.65
CBV.dial PRV @14:43:22.26 PSV.dial :mixloop @14:43:23.29 CTV.dial :air,avoiding: :intake @14:43:23.47 PTV.dial :PRVmixing @14:43:24.21 PS.seek 0ml @14:43:50.55 PSV.dial :air @14:43:51.01 PS.seek 2.5ml @14:44:17.39 PSV.dial :mixloop @14:44:17.87 PS.seek 0ml @14:44:44.24 PSV.dial :air @14:44:44.82 PS.seek 2.5ml @14:45:11.17 PSV.dial :mixloop @14:45:11.63 PS.seek 0ml @14:45:37.96 Garaging FlushPuck in Collection Clamp @14:45:38.03 Elbow.reconfigure :tight @14:45:38.08 Elbow.seek CC @14:45:38.33 Elbow.reconfigure :loose @14:45:38.43 Forearm.seek CC @14:45:39.44 Hand.close @14:45:40.10 CC.seek :open @14:45:44.72 Forearm.seek :retract @14:45:45.68 Elbow.reconfigure :tight @14:45:45.73 Elbow.seek :garage @14:45:48.67 Elbow.reconfigure :loose @14:45:48.77 Forearm.reconfigure :tight @14:45:48.82 Forearm.seek :garage @14:45:49.83 Hand.open @14:45:50.54 Forearm.reconfigure :loose @14:45:50.59 Forearm.seek :retract @14:45:51.56 delayUntil "14:49:59.28" @14:45:51.58 Whole Cell Archive sampling 50ml @14:45:51.60 Loading wcr puck from tube 2 into Collection Clamp @14:45:52.29 SC.reconfigure :tight @14:45:52.35 SC.seek 2 @14:45:52.66 SC.configure :limp @14:45:52.89 Elbow.reconfigure :tight @14:45:52.95 Elbow.seek 2 @14:45:54.86 Forearm.reconfigure :tight @14:45:54.91 Forearm.seek 2 + 450 counts @14:45:55.90 SE.reconfigure :pick @14:45:55.96 SE.seek :beamBreak @14:45:59.02 SE.configure :default @14:45:59.19 Elbow.seek 2 @14:45:59.39 Forearm.seek 2 + 300 counts @14:45:59.69 Forearm.reconfigure :loose @14:45:59.75 Hand.close @14:46:00.36 Puck::Warning in WCR -- Loaded Last remaining Puck (from Tube 2) @14:46:00.38 SE.seek :down @14:46:00.60 Forearm.seek :retract @14:46:01.54 Elbow.seek CC @14:46:03.17 SE.stop @14:46:03.19 Elbow.reconfigure :loose @14:46:03.34 Forearm.reconfigure :tight @14:46:03.39 Forearm.seek CC @14:46:04.68 CC.seek :closed @14:46:09.64 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.15 @14:46:09.66 Hand.open @14:46:10.29 Forearm.reconfigure :loose @14:46:10.34 Forearm.seek :retract @14:46:11.32 collectSample 50ml w/bubble point of 35psi @14:46:11.34 Priming 7ml @14:46:11.35 Intake.select :close @14:46:11.36 Exhaust.select :close @14:46:11.37 SS.hold @14:46:11.59 Calibrating pressure sensors @14:46:11.60 CTV.dial :air @14:46:11.62 SSV.dial :spit,avoiding: :exhaust @14:46:11.71 SS.reconfigure :fast @14:46:11.76 SS.seek 5ml @14:46:14.44 SSV.dial CBV @14:46:14.47 CBV.dial SSV @14:46:15.83 delay 3.8 seconds @14:46:19.76 Sensor limits: Intake max 121.5psia; Outlet max 116.1psia @14:46:19.77 SSV.dial :spit @14:46:21.09 SS.seek 0ml @14:46:23.82 SSV.dial CBV @14:46:25.16 CTV.dial :intake @14:46:25.92 SSV.dial CBV @14:46:25.94 CBV.dial SSV @14:46:26.02 Allowing 40s for 7.0ml of remaining 7.0ml @14:46:26.04 Intake.select :open @14:46:28.21 Intake @15.8psia, vacuum from 10.0 to 13.0psi @14:46:28.23 SS.reconfigure :inhaling 2.20ml/s @14:46:28.28 SS.seek 7ml @14:46:35.03 Stroke completed -- waiting for pressure to equalize ... @14:46:35.04 SS.reconfigure :exhale @14:46:35.09 SS.hold @14:46:40.96 Intake.select :close @14:46:41.12 SSV.dial :exhaust @14:46:41.89 Exhaust.select :open @14:46:42.00 SS.seek 0ml @14:46:46.18 Exhaust.select :close @14:46:46.30 Sampling 50.0ml @14:46:46.31 Intake.select :close @14:46:46.32 Exhaust.select :close @14:46:46.34 SS.reconfigure :default @14:46:46.38 SS.hold @14:46:46.60 CTV.dial :intake @14:46:46.84 SSV.dial CBV @14:46:46.87 CBV.dial SSV @14:46:47.64 Allowing 130s for 25.0ml of remaining 50.0ml @14:46:47.65 Intake.select :open @14:46:49.83 Intake @16.3psia, vacuum from 10.0 to 13.0psi @14:46:49.85 SS.reconfigure :inhaling 2.20ml/s @14:46:49.90 SS.seek 25ml @14:46:59.35 Vacuum too high -- waiting up to 40 seconds @14:46:59.36 SS.reconfigure :exhale @14:46:59.42 SS.hold @14:47:00.69 Intake @13.8psia, vacuum from 8.3 to 11.3psi @14:47:00.71 SS.reconfigure :inhaling 1.76ml/s @14:47:00.76 SS.seek 25ml @14:47:07.93 Stroke completed -- waiting for pressure to equalize ... @14:47:07.94 SS.reconfigure :exhale @14:47:07.99 SS.hold @14:47:13.32 Intake.select :close @14:47:13.48 SSV.dial :exhaust @14:47:14.25 Exhaust.select :open @14:47:14.37 SS.seek 0ml @14:47:23.21 Exhaust.select :close @14:47:23.33 SSV.dial CBV @14:47:23.35 CBV.dial SSV @14:47:24.11 Allowing 130s for 25.0ml of remaining 25.0ml @14:47:24.12 Intake.select :open @14:47:26.28 Intake @16.4psia, vacuum from 10.0 to 13.0psi @14:47:26.30 SS.reconfigure :inhaling 1.76ml/s @14:47:26.35 SS.seek 25ml @14:47:37.57 Vacuum too high -- waiting up to 40 seconds @14:47:37.58 SS.reconfigure :exhale @14:47:37.64 SS.hold @14:47:39.92 Intake @14.9psia, vacuum from 9.4 to 12.4psi @14:47:39.94 SS.reconfigure :inhaling 1.41ml/s @14:47:39.99 SS.seek 25ml @14:47:48.74 Stroke completed -- waiting for pressure to equalize ... @14:47:48.76 SS.reconfigure :exhale @14:47:48.80 SS.hold @14:47:53.98 Intake.select :close @14:47:54.14 SSV.dial :exhaust @14:47:54.89 Exhaust.select :open @14:47:55.00 SS.seek 0ml @14:48:03.78 Exhaust.select :close @14:48:03.89 Sampled 50.0ml @14:48:03.90 Drying puck before fixing for 4:00 @14:48:03.91 CBV.dial :waste2b @14:48:04.67 delay 1:00 @14:49:04.69 CSV.dial :air @14:49:05.15 CS.reconfigure :fast @14:49:05.21 CS.seek 10ml @14:49:30.10 Pumping wcr puck from tube 2 up to 35psi... @14:49:30.12 CBV.dial SSV @14:49:31.37 CBV.dial between CSV and waste2b @14:49:32.33 CSV.dial between air and CTV @14:49:32.66 CS.seek 7.25ml @14:49:39.79 CTV.dial CSV,avoiding: :air @14:49:39.82 CSV.dial CTV @14:49:40.65 CS.reconfigure :pressurize @14:49:40.71 CS.seek 0ml @14:49:56.12 CBV.dial :waste2b @14:49:56.45 CS.reconfigure :default @14:49:56.95 CS.seek 0ml @14:49:59.29 recoverPRVreagent08 @14:49:59.30 PTV.dial :mixing @14:50:00.06 PBV.dial :waste1 @14:50:00.44 delay 30 seconds @14:50:01.51 delay 1:00 @14:50:30.46 PBV.dial between PSV and waste1 @14:50:30.83 PSV.dial :air @14:50:31.30 PS.reconfigure :fast @14:50:31.35 PS.seek 2.5ml @14:50:55.29 PSV.dial :mixloop @14:50:55.76 delay 2 seconds @14:50:57.78 PSV.dial :air @14:50:58.25 delay 2 seconds @14:51:00.27 PSV.dial :mixloop @14:51:00.75 delay 2 seconds @14:51:01.53 CTV.dial :air,avoiding: :intake @14:51:02.76 PSV.dial :air @14:51:02.84 Fix #1 of 1 (with RNAlater) for 4:00 @14:51:02.86 CSV.dial CSR @14:51:03.24 PS.seek 0ml @14:51:03.31 Pulling 2ml of CSR.RNAlater @14:51:03.33 CSR.select :RNAlater @14:51:03.44 CS.seek 2ml @14:51:09.66 delay 5 seconds @14:51:14.68 CSR.select :air @14:51:14.80 CS.reconfigure :fast @14:51:14.86 CS.seek 10ml @14:51:26.76 washCycleDA #1 of 2 @14:51:26.78 PSR.select :air @14:51:26.79 PSV.dial PSR @14:51:28.11 Pulling 0.5ml of PSR.wash @14:51:28.12 PSR.select :wash @14:51:28.24 PS.reconfigure :default @14:51:28.28 PS.seek 0.5ml @14:51:34.42 delay 5 seconds @14:51:34.95 Pumping wcr puck from tube 2 up to 35psi... @14:51:34.96 CBV.dial SSV @14:51:36.20 CBV.dial between CSV and waste2b @14:51:37.16 CSV.dial between air and CTV @14:51:37.76 CS.seek 7.317ml @14:51:39.43 PSR.select :air @14:51:39.56 PS.seek 2ml @14:51:44.85 CTV.dial CSV,avoiding: :air @14:51:44.88 CSV.dial CTV @14:51:46.26 CS.reconfigure :pressurize @14:51:46.31 CS.seek 0ml @14:51:55.70 PTV.dial :puck @14:51:56.43 PSV.dial PTV @14:51:57.18 PBV.dial :waste1 @14:51:57.76 PS.seek 1.5ml @14:52:03.76 delay 3 seconds @14:52:04.43 delay 4:00 @14:52:06.78 PBV.dial between PSV and waste1 @14:52:07.14 PS.seek 0ml @14:52:23.51 delay 2:00 @14:54:23.53 recoverPSRreagent08 @14:54:23.54 PTV.dial :puck @14:54:23.55 PBV.dial :waste1 @14:54:23.93 delay 30 seconds @14:54:53.96 PBV.dial between PSV and waste1 @14:54:54.33 PSR.select :air @14:54:54.35 PSV.dial PSR @14:54:55.12 PS.reconfigure :fast @14:54:55.17 PS.seek 2.5ml @14:55:19.13 PSV.dial PTV @14:55:19.88 delay 5 seconds @14:55:24.90 PSV.dial PSR @14:55:25.65 delay 5 seconds @14:55:30.67 PSV.dial PTV @14:55:31.41 delay 5 seconds @14:55:36.43 PSV.dial :air @14:55:37.33 PS.seek 0ml @14:56:00.82 washCycleDA #2 of 2 @14:56:00.83 PSR.select :air @14:56:00.85 PSV.dial PSR @14:56:02.18 Pulling 0.5ml of PSR.wash @14:56:02.19 PSR.select :wash @14:56:02.31 PS.reconfigure :default @14:56:02.36 PS.seek 0.5ml @14:56:04.45 CBV.dial :waste2b @14:56:04.79 CS.reconfigure :default @14:56:04.84 CS.seek 0ml @14:56:06.84 delay 1:00 @14:56:08.35 delay 5 seconds @14:56:13.37 PSR.select :air @14:56:13.49 PS.seek 2ml @14:56:29.66 PTV.dial :puck @14:56:29.68 PSV.dial PTV @14:56:30.55 PBV.dial :waste1 @14:56:30.99 PS.seek 1.5ml @14:56:36.88 delay 3 seconds @14:56:39.90 PBV.dial between PSV and waste1 @14:56:40.26 PS.seek 0ml @14:56:56.35 delay 2:00 @14:57:06.86 CTV.dial :air,avoiding: :intake @14:58:56.38 recoverPSRreagent08 @14:58:56.39 PTV.dial :puck @14:58:56.40 PBV.dial :waste1 @14:58:56.77 delay 30 seconds @14:59:26.80 PBV.dial between PSV and waste1 @14:59:27.17 PSR.select :air @14:59:27.19 PSV.dial PSR @14:59:27.93 PS.reconfigure :fast @14:59:27.98 PS.seek 2.5ml @14:59:51.69 PSV.dial PTV @14:59:52.44 delay 5 seconds @14:59:57.46 PSV.dial PSR @14:59:58.22 delay 5 seconds @15:00:03.24 PSV.dial PTV @15:00:03.99 delay 5 seconds @15:00:09.01 PSV.dial :air @15:00:10.21 PS.seek 0ml @15:00:33.53 getPMLReagent08 @15:00:33.54 PRV.dial :air @15:00:34.28 delay 5 seconds @15:00:39.30 PTV.dial :PRVmixing @15:00:40.63 delay 5 seconds @15:00:45.65 PRV.dial :ab2 @15:00:46.09 PTV.dial :PRVmixing @15:00:46.17 PSV.dial :mixloop @15:00:46.65 PS.reconfigure :default @15:00:46.70 PS.seek 0.3ml @15:00:50.70 delay 2 seconds @15:00:52.71 PRV.dial :air @15:00:53.24 PS.seek 0.55ml @15:00:56.66 addPMLReagent08 @15:00:56.67 PSV.dial :air @15:00:57.15 PS.seek 2ml @15:01:12.97 PSV.dial :PML @15:01:13.44 PTV.dial :mixing @15:01:14.19 PBV.dial :waste1 @15:01:14.62 PS.seek 1.85ml @15:01:17.03 PBV.dial between PSV and waste1 @15:01:17.40 PS.seek 0ml @15:01:37.16 delay 2 seconds @15:01:39.17 delay 8:00 @15:09:39.20 recoverPRVreagent08 @15:09:39.21 PTV.dial :mixing @15:09:39.23 PBV.dial :waste1 @15:09:39.60 delay 30 seconds @15:10:09.63 PBV.dial between PSV and waste1 @15:10:10.01 PSV.dial :air @15:10:10.48 PS.reconfigure :fast @15:10:10.53 PS.seek 2.5ml @15:10:34.43 PSV.dial :mixloop @15:10:34.90 delay 2 seconds @15:10:36.91 PSV.dial :air @15:10:37.38 delay 2 seconds @15:10:39.40 PSV.dial :mixloop @15:10:39.87 delay 2 seconds @15:10:41.88 PSV.dial :air @15:10:42.35 PS.seek 0ml @15:11:05.65 washCycleDA #1 of 3 @15:11:05.66 PSR.select :air @15:11:05.68 PSV.dial PSR @15:11:06.99 Pulling 0.5ml of PSR.wash @15:11:07.00 PSR.select :wash @15:11:07.12 PS.reconfigure :default @15:11:07.17 PS.seek 0.5ml @15:11:13.05 delay 5 seconds @15:11:18.07 PSR.select :air @15:11:18.19 PS.seek 2ml @15:11:34.33 PTV.dial :puck @15:11:35.07 PSV.dial PTV @15:11:35.80 PBV.dial :waste1 @15:11:36.24 PS.seek 1.5ml @15:11:42.21 delay 3 seconds @15:11:45.22 PBV.dial between PSV and waste1 @15:11:45.60 PS.seek 0ml @15:12:01.74 delay 2:00 @15:14:01.77 recoverPSRreagent08 @15:14:01.78 PTV.dial :puck @15:14:02.31 PBV.dial :waste1 @15:14:02.69 delay 30 seconds @15:14:32.71 PBV.dial between PSV and waste1 @15:14:33.06 PSR.select :air @15:14:33.08 PSV.dial PSR @15:14:33.83 PS.reconfigure :fast @15:14:33.88 PS.seek 2.5ml @15:14:57.83 PSV.dial PTV @15:14:58.58 delay 5 seconds @15:15:03.60 PSV.dial PSR @15:15:04.35 delay 5 seconds @15:15:09.37 PSV.dial PTV @15:15:10.13 delay 5 seconds @15:15:15.15 PSV.dial :air @15:15:15.90 PS.seek 0ml @15:15:39.33 washCycleDA #2 of 3 @15:15:39.34 PSR.select :air @15:15:39.36 PSV.dial PSR @15:15:40.67 Pulling 0.5ml of PSR.wash @15:15:40.68 PSR.select :wash @15:15:40.80 PS.reconfigure :default @15:15:40.85 PS.seek 0.5ml @15:15:46.86 delay 5 seconds @15:15:51.88 PSR.select :air @15:15:52.00 PS.seek 2ml @15:16:08.14 PTV.dial :puck @15:16:08.22 PSV.dial PTV @15:16:08.95 PBV.dial :waste1 @15:16:09.37 PS.seek 1.5ml @15:16:15.36 delay 3 seconds @15:16:18.38 PBV.dial between PSV and waste1 @15:16:18.74 PS.seek 0ml @15:16:35.08 delay 2:00 @15:18:35.11 recoverPSRreagent08 @15:18:35.12 PTV.dial :puck @15:18:35.18 PBV.dial :waste1 @15:18:35.54 delay 30 seconds @15:19:05.57 PBV.dial between PSV and waste1 @15:19:05.93 PSR.select :air @15:19:05.95 PSV.dial PSR @15:19:06.70 PS.reconfigure :fast @15:19:06.75 PS.seek 2.5ml @15:19:30.63 PSV.dial PTV @15:19:31.51 delay 5 seconds @15:19:36.53 PSV.dial PSR @15:19:37.26 delay 5 seconds @15:19:42.28 PSV.dial PTV @15:19:43.04 delay 5 seconds @15:19:48.06 PSV.dial :air @15:19:48.81 PS.seek 0ml @15:20:12.39 washCycleDA #3 of 3 @15:20:12.40 PSR.select :air @15:20:12.41 PSV.dial PSR @15:20:13.73 Pulling 0.5ml of PSR.wash @15:20:13.74 PSR.select :wash @15:20:13.86 PS.reconfigure :default @15:20:13.91 PS.seek 0.5ml @15:20:19.79 delay 5 seconds @15:20:24.81 PSR.select :air @15:20:24.93 PS.seek 2ml @15:20:41.23 PTV.dial :puck @15:20:41.31 PSV.dial PTV @15:20:42.05 PBV.dial :waste1 @15:20:42.95 PS.seek 1.5ml @15:20:48.91 delay 3 seconds @15:20:51.93 PBV.dial between PSV and waste1 @15:20:52.30 PS.seek 0ml @15:21:08.39 delay 2:00 @15:23:08.41 recoverPSRreagent08 @15:23:08.43 PTV.dial :puck @15:23:08.51 PBV.dial :waste1 @15:23:08.87 delay 30 seconds @15:23:38.89 PBV.dial between PSV and waste1 @15:23:39.27 PSR.select :air @15:23:39.29 PSV.dial PSR @15:23:40.04 PS.reconfigure :fast @15:23:40.09 PS.seek 2.5ml @15:24:03.94 PSV.dial PTV @15:24:04.69 delay 5 seconds @15:24:09.71 PSV.dial PSR @15:24:10.46 delay 5 seconds @15:24:15.48 PSV.dial PTV @15:24:16.24 delay 5 seconds @15:24:21.26 PSV.dial :air @15:24:22.03 PS.seek 0ml @15:24:45.53 Gate.power :camera,:ON @15:24:45.55 Mixing DA Substrates @15:24:45.56 PSR.select :air @15:24:45.58 PSV.dial PSR @15:24:46.95 Pulling 0.25ml of PSR.sub1 @15:24:46.97 PSR.select :sub1 @15:24:47.17 PS.reconfigure :default @15:24:47.30 PS.seek 0.25ml @15:24:50.73 delay 5 seconds @15:24:55.75 Pulling 0.25ml of PSR.sub2 @15:24:55.77 PSR.select :sub2 @15:24:55.89 PS.seek 0.5ml @15:24:59.28 delay 5 seconds @15:25:04.31 PSR.select :air @15:25:04.42 PS.seek 2ml @15:25:20.75 PTV.dial :puck @15:25:20.83 PSV.dial PTV @15:25:21.58 PBV.dial :waste1 @15:25:22.02 PS.seek 1.5ml @15:25:28.00 delay 3 seconds @15:25:31.02 PBV.dial between PSV and waste1 @15:25:31.40 PS.seek 0ml @15:25:47.56 delay 10 seconds @15:25:57.59 recoverPSRreagent08 @15:25:57.60 PTV.dial :puck @15:25:57.67 PBV.dial :waste1 @15:25:58.05 delay 30 seconds @15:26:28.08 PBV.dial between PSV and waste1 @15:26:28.45 PSR.select :air @15:26:28.47 PSV.dial PSR @15:26:29.24 PS.reconfigure :fast @15:26:29.29 PS.seek 2.5ml @15:26:53.59 PSV.dial PTV @15:26:54.36 delay 5 seconds @15:26:59.38 PSV.dial PSR @15:27:00.16 delay 5 seconds @15:27:05.18 PSV.dial PTV @15:27:05.94 delay 5 seconds @15:27:10.96 PSV.dial :air @15:27:11.73 PS.seek 0ml @15:27:35.63 Additional air clear @15:27:35.64 PSR.select :air @15:27:36.08 PSV.dial PSR @15:27:37.47 Pulling 2ml of PSR.air @15:27:37.48 PSR.select :air @15:27:37.50 PS.reconfigure :default @15:27:37.55 PS.seek 2ml @15:27:58.75 delay 5 seconds @15:28:03.77 PTV.dial :puck @15:28:03.86 PSV.dial PTV @15:28:04.61 PBV.dial :waste1 @15:28:05.05 PS.seek 1.5ml @15:28:11.00 delay 3 seconds @15:28:14.02 PBV.dial between PSV and waste1 @15:28:14.39 delay 1 second @15:28:15.41 PSV.dial :waste1 @15:28:16.48 PS.seek 0ml @15:28:32.68 Processing Puck Heater at 28.1C before imaging @15:28:32.69 PH.coast @15:28:32.80 Imaging da puck in Processing Clamp while storing it to tube 1 @15:28:32.98 SC.reconfigure :tight @15:28:33.03 SC.seek 1 @15:28:36.57 SC.configure :limp @15:28:36.80 Elbow.reconfigure :tight @15:28:36.85 Elbow.seek 1 @15:28:38.67 Forearm.reconfigure :tight @15:28:38.72 Forearm.seek 1 + 450 counts @15:28:39.74 SE.reconfigure :pick @15:28:39.80 SE.seek :beamBreak @15:28:42.86 SE.configure :default @15:28:43.02 Elbow.reconfigure :limp @15:28:43.07 Forearm.reconfigure :loose @15:28:43.12 Forearm.seek 1 @15:28:43.35 Elbow.hold @15:28:43.41 Elbow.reconfigure :tight @15:28:43.51 SE.seek 1.17 Pucks below Top Plate @15:28:43.95 Forearm.force -20 @15:28:43.99 Forearm.seek 1 + 318 counts @15:28:44.29 Forearm.reconfigure :tight @15:28:44.34 Forearm.seek 1 + 450 counts @15:28:44.55 SE.seek 2.17 Pucks below Top Plate @15:28:45.69 Forearm.reconfigure :loose @15:28:45.74 Forearm.seek :retract @15:28:46.66 Elbow.seek PC @15:28:49.17 Elbow.reconfigure :loose @15:28:49.27 Forearm.seek PC @15:28:50.20 Hand.close @15:28:50.82 PC.seek :open @15:28:55.64 Forearm.seek :retract @15:28:56.63 Elbow.reconfigure :tight @15:28:56.69 Elbow.seek 1 @15:28:59.31 Forearm.reconfigure :tight @15:28:59.36 Forearm.seek 1 + 450 counts @15:29:00.34 Hand.open @15:29:01.00 SE.seek 1.82 Pucks below Top Plate @15:29:01.28 SE.reconfigure :seal @15:29:01.33 SE.seek 0.85 Pucks below Top Plate @15:29:01.95 Lights, camera, ACTION! @15:29:01.99 PuckCamera: snap -bin=2,2 -auto /var/log/esp/da24jan2215h50ml.tif @15:29:21.39 Waiting >20 seconds for da to relinquish FlushPuck @15:29:31.91 SX HX9/16 (USB:06): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 26.618 seconds /var/log/esp/da24jan2215h50ml.tif: TIFF Upload Complete @15:29:31.94 PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da24jan2215h50ml15s.tif @15:29:47.58 SX HX9/16 (USB:06): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15 seconds /var/log/esp/midres/da24jan2215h50ml15s.tif: TIFF Upload Complete @15:29:47.61 PuckCamera: snap -bin=2,2 20.0 /var/log/esp/midres/da24jan2215h50ml20s.tif @15:30:08.23 SX HX9/16 (USB:06): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 20 seconds /var/log/esp/midres/da24jan2215h50ml20s.tif: TIFF Upload Complete @15:30:08.24 Gate.power :camera,:OFF @15:30:08.26 DAfans.email "26.618 sec auto-exposure",Subject: "da24jan2215h50ml images!" @15:30:08.70 Elbow.seek 1 @15:30:08.99 Forearm.seek 1 + 450 counts @15:30:09.09 SE.reconfigure :gently @15:30:09.15 SE.seek 1.57 Pucks below Top Plate @15:30:09.84 Elbow.reconfigure :limp @15:30:09.91 Forearm.reconfigure :loose @15:30:09.96 Forearm.seek 1 @15:30:10.22 Elbow.hold @15:30:10.29 Elbow.reconfigure :tight @15:30:10.35 SE.reconfigure :default @15:30:10.98 SE.seek 1.75 Pucks below Top Plate @15:30:11.64 Forearm.force -20 @15:30:11.76 Forearm.seek 1 + 296 counts @15:30:12.27 Forearm.reconfigure :tight @15:30:12.33 Forearm.seek 1 + 450 counts @15:30:12.80 SE.seek :down @15:30:14.10 Forearm.reconfigure :loose @15:30:14.21 Forearm.seek :retract @15:30:15.45 SE.stop @15:30:15.68 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @15:30:16.30 Elbow.seek FlushPuck::Garage @15:30:18.28 Elbow.reconfigure :loose @15:30:18.50 Forearm.seek :garage @15:30:19.57 Hand.close @15:30:20.51 Forearm.seek :retract @15:30:21.57 Elbow.reconfigure :tight @15:30:21.65 Elbow.seek PC @15:30:23.10 Elbow.reconfigure :loose @15:30:23.31 Forearm.reconfigure :tight @15:30:23.41 Forearm.seek PC @15:30:24.97 PC.seek :closed @15:30:29.77 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.65 @15:30:29.90 Hand.open @15:30:31.14 upsync @15:30:31.50 Forearm.reconfigure :loose @15:30:31.56 Forearm.seek :retract @15:30:32.50 PSR.select :air @15:30:32.53 Storing wcr puck in Collection Clamp to tube 1 @15:30:32.55 PSV.dial PSR @15:30:32.81 SC.reconfigure :tight @15:30:32.86 SC.seek 1 @15:30:32.93 SC.configure :limp @15:30:33.10 Pulling 0.5ml of PSR.flush @15:30:33.15 PSR.select :flush @15:30:33.27 Elbow.reconfigure :tight @15:30:33.32 Elbow.seek 1 @15:30:33.38 PS.seek 0.5ml @15:30:35.97 Forearm.reconfigure :tight @15:30:36.02 Forearm.seek 1 + 450 counts @15:30:37.05 SE.reconfigure :pick @15:30:37.11 SE.seek :beamBreak @15:30:39.44 delay 5 seconds @15:30:40.19 SE.configure :default @15:30:40.35 Elbow.reconfigure :limp @15:30:40.41 Forearm.reconfigure :loose @15:30:40.46 Forearm.seek 1 @15:30:40.71 Elbow.hold @15:30:40.77 Elbow.reconfigure :tight @15:30:40.88 SE.seek 2.16 Pucks below Top Plate @15:30:41.48 Forearm.force -20 @15:30:41.53 Forearm.seek 1 + 305 counts @15:30:41.86 Forearm.reconfigure :tight @15:30:41.91 Forearm.seek 1 + 450 counts @15:30:42.10 SE.seek 3.16 Pucks below Top Plate @15:30:42.76 Forearm.reconfigure :loose @15:30:42.81 Forearm.seek :retract @15:30:43.80 Elbow.seek CC @15:30:44.46 PSR.select :air @15:30:44.58 PS.seek 2.5ml @15:30:45.44 Elbow.reconfigure :loose @15:30:45.54 Forearm.seek CC @15:30:46.55 Hand.close @15:30:47.19 CC.seek :open @15:30:51.99 Forearm.seek :retract @15:30:52.94 Elbow.reconfigure :tight @15:30:52.99 Elbow.seek 1 @15:30:54.76 Elbow.reconfigure :limp @15:30:54.82 Forearm.seek 1 @15:30:55.81 Elbow.hold @15:30:55.89 Elbow.reconfigure :tight @15:30:55.94 Hand.open @15:30:56.69 Forearm.force -20 @15:30:56.75 Forearm.seek 1 + 319 counts @15:30:57.06 Forearm.reconfigure :tight @15:30:57.11 Forearm.seek 1 + 450 counts @15:30:57.36 SE.seek :down @15:30:58.50 Forearm.reconfigure :loose @15:30:58.56 Forearm.seek :retract @15:30:59.93 SE.stop @15:30:59.97
Starting finalPurge @15:31:05.78 delay 1 second @15:31:06.85 PRV.dial :waste2,avoiding: :ab2 @15:31:08.70 PTV.dial :PRVmixing @15:31:10.01 PSV.dial :mixloop @15:31:11.05 PS.seek :empty @15:31:19.93
Waiting >20 seconds for psFlush to relinquish FlushPuck @15:31:37.37 PSR.select :air @15:31:37.39 PSV.dial PSR @15:31:38.43 Pulling 0.5ml of PSR.flush @15:31:38.45 PSR.select :flush @15:31:38.57 PS.seek 0.5ml @15:31:44.46 delay 5 seconds @15:31:49.49 PSR.select :air @15:31:49.61 PS.seek 2.5ml @15:32:10.81 delay 1 second @15:32:11.83 PBV.dial :waste1 @15:32:12.20 PTV.dial :mixing @15:32:12.93 PSV.dial :mixloop @15:32:13.98 PS.seek :empty @15:32:40.37 PSV.dial PSR @15:32:41.41 PSR.select :flush @15:32:41.54 PS.seek 1ml @15:32:52.62 PSR.select :air @15:32:52.74 PS.seek 2.5ml @15:33:08.90 PSV.dial PTV @15:33:09.66 PTV.dial :puck @15:33:10.40 PBV.dial :waste1 @15:33:10.58 PS.seek 0ml @15:33:36.96 PSV.dial PSR @15:33:37.71 PSR.select :flush @15:33:37.83 PS.seek 0.5ml @15:33:43.83 PSR.select :air @15:33:43.96 PS.seek 2.5ml @15:34:05.24 PSV.dial PTV @15:34:05.99 PBV.dial PSV @15:34:06.54 PS.seek 0ml @15:34:33.11 PSV.dial PBV @15:34:33.70 PS.seek 2.5ml @15:35:00.11 PSV.dial :waste1 @15:35:00.89 PS.seek 0ml @15:35:27.27 PSV.dial PSR @15:35:27.74 PSR.select :air @15:35:27.76 PS.seek 2.5ml @15:35:54.18 PSV.dial PTV @15:35:54.92 PBV.dial PSV @15:35:55.12 PS.seek 0ml @15:36:21.91 PSV.dial PBV @15:36:22.40 PS.seek 2.5ml @15:36:48.71 PSV.dial :waste1 @15:36:49.45 PS.seek 0ml @15:37:15.80 PSV.dial PSR @15:37:16.27 PSR.select :flush @15:37:16.40 PS.seek 1ml @15:37:27.55 PSR.select :air @15:37:27.67 PS.seek 2.5ml @15:37:43.77 PSV.dial PTV @15:37:44.50 PTV.dial :puck @15:37:44.69 PBV.dial :waste1 @15:37:45.23 PS.seek 0ml @15:38:11.64 PSV.dial PSR @15:38:12.39 PSR.select :flush @15:38:12.52 PS.seek 0.5ml @15:38:18.39 PSR.select :air @15:38:18.51 PS.seek 2.5ml @15:38:39.83 PSV.dial PTV @15:38:40.58 PBV.dial PSV @15:38:41.13 PS.seek 0ml @15:39:07.67 PSV.dial PBV @15:39:08.15 PS.seek 2.5ml @15:39:34.55 PSV.dial :waste1 @15:39:35.29 PS.seek 0ml @15:40:01.83 PSV.dial PSR @15:40:02.30 PSR.select :air @15:40:02.32 PS.seek 2.5ml @15:40:28.61 PSV.dial PTV @15:40:29.34 PBV.dial PSV @15:40:29.43 PS.seek 0ml @15:40:55.76 PSV.dial PBV @15:40:56.24 PS.seek 2.5ml @15:41:22.67 PSV.dial :waste1 @15:41:23.41 PS.seek 0ml @15:41:49.83
Loading FlushPuck into Collection Clamp from Processing Clamp @15:41:50.02 Elbow.seek PC @15:41:52.53 Elbow.reconfigure :loose @15:41:52.63 Forearm.seek PC @15:41:53.58 Hand.close @15:41:54.56 PC.seek :open @15:41:59.39 Forearm.seek :retract @15:42:00.35 Elbow.reconfigure :tight @15:42:00.40 Elbow.seek CC @15:42:03.97 Elbow.reconfigure :loose @15:42:04.07 Forearm.reconfigure :tight @15:42:04.13 Forearm.seek CC @15:42:05.33 CC.seek :closed @15:42:09.85 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.40 @15:42:09.92 Hand.open @15:42:10.62 Forearm.reconfigure :loose @15:42:10.66 Forearm.seek :retract @15:42:11.62 Flushing the Collection Valve::Manifold @15:42:11.74 CSV.dial CSR @15:42:12.19 Pulling 5ml of CSR.flush @15:42:12.20 CSR.select :flush @15:42:12.32 CS.seek 5ml @15:42:26.11 delay 5 seconds @15:42:31.13 CSR.select :air @15:42:31.30 CS.reconfigure :fast @15:42:31.35 CS.seek 7.5ml @15:42:38.20 CSV.dial :waste @15:42:39.49 CS.reconfigure :default @15:42:39.54 CS.seek 0ml @15:42:59.58 Flushing lower manifold's waste1 port @15:42:59.70 CSV.dial :flush @15:43:00.27 CS.reconfigure :fast @15:43:00.32 CS.seek 5ml @15:43:13.42 delay 5 seconds @15:43:18.44 CSV.dial :air @15:43:19.09 CS.seek 7ml @15:43:24.89 CSV.dial CTV @15:43:24.92
CTV.dial CSV @15:43:26.75 CBV.dial :waste1 @15:43:27.50 CS.seek 0ml @15:43:44.39 Flushing lower manifold's waste2 port @15:43:44.52 CSV.dial :flush @15:43:45.26 CS.seek 5ml @15:43:58.33 delay 5 seconds @15:44:03.35 CSV.dial :air @15:44:03.87 CS.seek 7ml @15:44:09.73 CSV.dial CTV @15:44:09.76
CTV.dial CSV @15:44:10.22 CBV.dial :waste2 @15:44:11.27 CS.seek 0ml @15:44:28.14 Flushing lower manifold's waste2b port @15:44:28.26 CSV.dial :flush @15:44:29.00 CS.seek 5ml @15:44:41.98 delay 5 seconds @15:44:47.00 CSV.dial :air @15:44:47.51 CS.seek 7.001ml @15:44:53.33 CSV.dial CTV @15:44:53.35
CTV.dial CSV @15:44:53.82 CBV.dial :waste2b @15:44:54.88 CS.seek 0ml @15:45:11.75 Disinfecting external Intake with kill @15:45:12.00 CSV.dial CSR @15:45:12.45 Pulling 8ml of CSR.kill @15:45:12.46 CSR.select :kill @15:45:12.58 CS.seek 8ml @15:45:32.76 delay 5 seconds @15:45:37.78 CSR.select :air @15:45:37.90 CS.seek 10ml @15:45:43.55 CSV.dial CBV @15:45:43.57
CBV.dial CSV @15:45:44.75 CTV.dial :intake @15:45:45.51 Intake.select :open @15:45:45.64 CS.seek 2ml @15:46:04.98 Intake.select :close @15:46:05.08 CTV.dial :air @15:46:05.83 CS.reconfigure :default @15:46:05.88 CS.seek 0ml @15:46:11.87 CSV.dial CSR @15:46:12.89 Pulling 5ml of CSR.flush @15:46:12.90 CSR.select :flush @15:46:13.04 CS.seek 5ml @15:46:26.64 delay 5 seconds @15:46:31.65 CSR.select :air @15:46:31.77 CS.seek 10ml @15:46:45.42 CSV.dial CBV @15:46:46.55 CS.seek 0ml @15:47:13.42 Garaging FlushPuck in Collection Clamp @15:47:13.49 Elbow.reconfigure :tight @15:47:13.54 Elbow.seek CC @15:47:13.83 Elbow.reconfigure :loose @15:47:13.93 Forearm.seek CC @15:47:14.97 Hand.close @15:47:15.55 CC.seek :open @15:47:20.22 Forearm.seek :retract @15:47:21.21 Elbow.reconfigure :tight @15:47:21.26 Elbow.seek :garage @15:47:24.10 Elbow.reconfigure :loose @15:47:24.20 Forearm.reconfigure :tight @15:47:24.25 Forearm.seek :garage @15:47:25.29 Hand.open @15:47:25.98 Forearm.reconfigure :loose @15:47:26.03 Forearm.seek :retract @15:47:27.02 Completed [initialPurge; da [50, 50] #da and wsr are run simultanelously] begun 13:56:36 @15:47:27.07 Asleep after completing DA_WCR_011024 assays @15:47:27.09 GoodNews.email "{1 => 4,\n 2 => 0,\n 3 => 0,\n 4 => 0,\n 5 => 0,\n 6 => 0,\n 7 => 0}\nCTD data unavailable\nCan@15:45:49, 26% flow, 23.4C, 8% humidity, 15.2psia, 13.622V, 0.598A, 85.502Ah, 8.15W\nSampled 50.0ml",Subject: "Asleep after completing DA_WCR_011024 assays" @15:47:31.00 upsync + ESPwhich DA_WCR_011024.rb + fn=DA_WCR_011024.rb + IFS=: + set /home/esp/esp2/mission . + fullPath=/home/esp/esp2/mission/DA_WCR_011024.rb + [ -f /home/esp/esp2/mission/DA_WCR_011024.rb ] + realpath /home/esp/esp2/mission/DA_WCR_011024.rb + return 0 + scriptPath=/home/esp/esp2local/mission/DA_WCR_011024.rb + exec ruby /home/esp/esp2/lib/esp.rb /home/esp/esp2local/mission/DA_WCR_011024.rb @13:57:55.99UTC23-Jan-24
ESP2 v3.42 I2C Gateway v3.20 for core bus @13:57:58.66 Configuration for short puck jake 1/11/21 by brent@mbari.org /home/esp/esp2/type/shallow/jake/configure.rb @13:58:02.31 Polling Can every 10:00 @13:58:02.33 Cleanly exited at 13:57:22UTC23-Jan-24 @13:58:02.38 Loading /home/esp/esp2local/mission/DA_WCR_011024.rb mission @13:58:02.44 Preparing to sleep until + 00:00:05 @13:58:02.47 GoodNews.email "Starting DA_WCR_011024 w/pucks in tube 2\n{3 => 0,\n 4 => 0,\n 5 => 0,\n 6 => 0,\n 7 => 0}\nCTD data unavailable\nCan@13:58:02, 26% flow, 22.3C, 13% humidity, 15.1psia, 13.739V, 0.351A, 92.393Ah, 4.82W",Subject: "DA_WCR_011024 begun" @13:58:02.52
Asleep til + 00:00:05 @13:58:02.54 Continuing at 13:58 @13:58:02.82 Gate.power :core,:OFF @13:58:02.84 Gate.power :raw,:OFF @13:58:02.85 Shutdown @13:58:05.27 GoodNews.email "Then, in tube 2: initialPurge; da [50, 50] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_011024.rb:14\n{1 => 0,\n 2 => 4,\n 3 => 0,\n 4 => 0,\n 5 => 0,\n 6 => 0,\n 7 => 0}\nCTD data unavailable\nCan@13:58:02, 26% flow, 22.3C, 13% humidity, 15.1psia, 13.734V, 0.375A, 92.393Ah, 5.15W",Subject: "Asleep until 13:58" @13:58:06.62 upsync @13:58:07.42
Powering core bus @13:58:09.21 upsync @13:58:12.60
Gate.power :raw,:ON @13:58:13.97 CS.configure :default @13:58:14.02 PS.configure :default @13:58:14.07 SS.configure :default @13:58:14.11 CC.configure :default @13:58:14.16 PC.configure :default @13:58:14.23 Forearm.configure :loose @13:58:14.28 Elbow.configure :loose @13:58:14.35 SC.configure :loose @13:58:14.39 SE.configure :default @13:58:14.44 CH.configure :default @13:58:14.48 PH.configure :default @13:58:14.52 All dwarf controllers running firmware version 3.85 @13:58:14.62 Valve.reset @13:58:14.90 Intake.select :close @13:58:14.94 Exhaust.select :close @13:58:14.95 CTV.dial :air @13:58:15.03 PSV.dial :air @13:58:15.09 CSV.dial :waste1 @13:58:15.21
Forearm.home.seek :retract @13:58:15.59 SSV.dial :spit,avoiding: :exhaust @13:58:15.92 Hand.close @13:58:15.95
Elbow.reconfigure :loose @13:58:16.04 Elbow.jog 1500 @13:58:16.18 PS.home.seek 0ml @13:58:16.46 CS.reconfigure :fast @13:58:16.50 SS.home.seek 0ml @13:58:16.57 CS.home.seek 0ml @13:58:16.67
Elbow.reconfigure :loose @13:58:16.73 Elbow.home.seek :clear @13:58:19.89 SE.home.seek :down @13:58:20.50 SC.reconfigure :tight @13:58:20.54 SC.home.seek :initial @13:58:24.51 PS.stop @13:58:45.74
SC.configure :limp @13:58:45.80 Forearm.seek :retract @13:58:45.86 Elbow.seek :hammer @13:58:46.99 Forearm.reconfigure :tight @13:58:47.04 Forearm.seek retracted @13:58:47.30 PC.home.seek :open @13:58:47.32
CC.home.seek :open @13:58:50.72 Phase [initialPurge; da [50, 50] #da and wsr are run simultanelously] @13:58:50.74 Notice.email "At 13:58:50 began in tube 2: initialPurge; da [50, 50] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_011024.rb:14\n{1 => 0,\n 2 => 4,\n 3 => 0,\n 4 => 0,\n 5 => 0,\n 6 => 0,\n 7 => 0}\nCTD data unavailable\nCan@13:58:50, 26% flow, 22.4C, 13% humidity, 15.1psia, 13.666V, 0.321A, 92.398Ah, 4.38W",Subject: "Started DA_WCR_011024 phase initialPurge; da [50, 50] #da and wsr are run simultanelously" @13:58:50.77
Starting initialPurge @13:58:50.79 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @13:58:50.85 Hand.open @13:58:51.61 Elbow.reconfigure :tight @13:58:51.65 Elbow.seek FlushPuck::Garage @13:58:53.65 Elbow.reconfigure :loose @13:58:53.79 Forearm.reconfigure :loose @13:58:53.84 Forearm.seek :garage @13:58:54.71 upsync @13:58:54.91
Hand.close @13:58:55.61 Forearm.seek :retract @13:58:56.61 Elbow.reconfigure :tight @13:58:56.66 Elbow.seek CC @13:58:59.62 Elbow.reconfigure :loose @13:58:59.72 Forearm.reconfigure :tight @13:58:59.77 Forearm.seek CC @13:59:01.09 CC.seek :closed @13:59:05.63 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.37 @13:59:05.70 Hand.open @13:59:06.42 Forearm.reconfigure :loose @13:59:06.47 Forearm.seek :retract @13:59:07.45 Flushing the Collection Valve::Manifold @13:59:07.58 CSV.dial CSR @13:59:08.87 Pulling 5ml of CSR.flush @13:59:08.88 CSR.select :flush @13:59:09.00 CS.reconfigure :default @13:59:09.04 CS.seek 5ml @13:59:22.84 delay 5 seconds @13:59:27.86 CSR.select :air @13:59:28.44 CS.reconfigure :fast @13:59:28.49 CS.seek 7.5ml @13:59:35.28 CSV.dial :waste @13:59:36.56 CS.reconfigure :default @13:59:36.61 CS.seek 0ml @13:59:56.56 Purging external Intake @13:59:56.68 CSV.dial :flush @13:59:57.13 CS.reconfigure :fast @13:59:57.17 CS.seek 10ml @14:00:21.74 delay 5 seconds @14:00:26.77 CSV.dial CBV @14:00:26.80
CBV.dial CSV @14:00:27.54 CTV.dial :intake @14:00:28.32 Intake.select :open @14:00:28.43 CS.seek 0ml @14:00:52.18 Intake.select :close @14:00:52.29 CTV.dial :air @14:00:53.06 Flushing upper manifold @14:00:53.18 CSV.dial :flush @14:00:53.92 CS.seek 5ml @14:01:06.65 delay 5 seconds @14:01:11.67 CSV.dial :air @14:01:12.17 CS.seek 7ml @14:01:17.78 CSV.dial CBV @14:01:17.80
CBV.dial CSV @14:01:18.84 CTV.dial :air @14:01:18.92 CS.seek 0ml @14:01:35.52 delay 7 seconds @14:01:42.55 CS.seek 3ml @14:01:50.62 CSV.dial :waste @14:01:51.08 CS.seek 0ml @14:01:59.07 CSV.dial :flush @14:01:59.51 CS.seek 3ml @14:02:07.74 delay 5 seconds @14:02:12.76 CSV.dial :air @14:02:13.26 CS.seek 5ml @14:02:18.93 CSV.dial :air @14:02:19.01 CS.seek 0ml @14:02:31.21 delay 7 seconds @14:02:38.24 CS.seek 3ml @14:02:46.27 CSV.dial :waste @14:02:47.01 CS.seek 0ml @14:02:54.78 Garaging FlushPuck in Collection Clamp @14:02:54.85 Elbow.reconfigure :tight @14:02:54.89 Elbow.seek CC @14:02:55.16 Elbow.reconfigure :loose @14:02:55.26 Forearm.seek CC @14:02:56.31 Hand.close @14:02:56.98 CC.seek :open @14:03:02.04 Forearm.seek :retract @14:03:03.04 Elbow.reconfigure :tight @14:03:03.09 Elbow.seek :garage @14:03:05.97 Elbow.reconfigure :loose @14:03:06.08 Forearm.reconfigure :tight @14:03:06.13 Forearm.seek :garage @14:03:07.15 Hand.open @14:03:07.79 Forearm.reconfigure :loose @14:03:07.84 Forearm.seek :retract @14:03:08.84 da sampling at most 50ml, wcr at most 50ml @14:03:08.88 Loading sh1 puck from tube 2 into Collection Clamp @14:03:08.90 DAfans.email "Started da in tube 2 at 14:03:08UTC23-Jan-24\n{1 => 0,\n 2 => 4,\n 3 => 0,\n 4 => 0,\n 5 => 0,\n 6 => 0,\n 7 => 0}\nCTD data unavailable\nCan@13:58:50, 26% flow, 22.4C, 13% humidity, 15.1psia, 13.666V, 0.321A, 92.398Ah, 4.38W",Subject: "da sampling at most 50ml, wcr at most 50ml" @14:03:09.16
SC.reconfigure :tight @14:03:09.23 SC.seek 2 @14:03:12.91 upsync @14:03:15.95
SC.configure :limp @14:03:16.18 Elbow.reconfigure :tight @14:03:16.23 Elbow.seek 2 @14:03:18.27 Forearm.reconfigure :tight @14:03:18.32 Forearm.seek 2 + 450 counts @14:03:19.36 SE.reconfigure :pick @14:03:19.42 SE.seek :beamBreak @14:03:22.34 SE.configure :default @14:03:22.50 Elbow.seek 2 @14:03:22.74 Forearm.seek 2 + 300 counts @14:03:22.92 Forearm.reconfigure :loose @14:03:22.96 Hand.close @14:03:23.62 SE.seek :down @14:03:23.82
Forearm.seek :retract @14:03:24.82 Elbow.seek CC @14:03:26.28 SE.stop @14:03:26.50
Elbow.reconfigure :loose @14:03:26.61 Forearm.reconfigure :tight @14:03:26.66 Forearm.seek CC @14:03:27.94 CC.seek :closed @14:03:32.47 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.23 @14:03:32.48 Hand.open @14:03:33.16 Forearm.reconfigure :loose @14:03:33.21 Forearm.seek :retract @14:03:34.21 collectSample 50ml w/bubble point of 24psi @14:03:34.23 Priming 7ml @14:03:34.24 Intake.select :close @14:03:34.25 Exhaust.select :close @14:03:34.26 SS.hold @14:03:34.47 Calibrating pressure and temperature sensors @14:03:34.50 CTV.dial :air @14:03:34.51 SSV.dial :spit,avoiding: :exhaust @14:03:34.70 SS.reconfigure :fast @14:03:34.74 SS.seek 5ml @14:03:37.71 SSV.dial CBV @14:03:37.74
CBV.dial SSV @14:03:39.05 delay 3.8 seconds @14:03:42.98 Sensor limits: Intake max 115.5psia; Outlet max 111.0psia @14:03:42.99 SSV.dial :spit @14:03:44.29 SS.seek 0ml @14:03:46.97 SSV.dial CBV @14:03:48.29 CTV.dial :intake @14:03:49.07 SSV.dial CBV @14:03:49.10
CBV.dial SSV @14:03:49.25 Allowing 40s for 7.0ml of remaining 7.0ml @14:03:49.26 Intake.select :open @14:03:51.43 Intake @15.6psia, vacuum from 10.0 to 13.0psi @14:03:51.45 SS.reconfigure :inhaling 2.20ml/s @14:03:51.49 SS.seek 7ml @14:03:58.59 Stroke completed -- waiting for pressure to equalize ... @14:03:58.60 SS.reconfigure :exhale @14:03:58.65 SS.hold @14:04:00.61 Intake.select :close @14:04:00.84 SSV.dial :exhaust @14:04:01.58 Exhaust.select :open @14:04:01.70 SS.seek 0ml @14:04:05.71 Exhaust.select :close @14:04:05.82 Sampling 50.0ml @14:04:05.83 Intake.select :close @14:04:05.84 Exhaust.select :close @14:04:05.85 SS.reconfigure :default @14:04:05.90 SS.hold @14:04:06.10 CTV.dial :intake @14:04:06.34 SSV.dial CBV @14:04:06.37
CBV.dial SSV @14:04:07.13 Allowing 130s for 25.0ml of remaining 50.0ml @14:04:07.14 Intake.select :open @14:04:09.31 Intake @15.9psia, vacuum from 10.0 to 13.0psi @14:04:09.33 SS.reconfigure :inhaling 2.20ml/s @14:04:09.38 SS.seek 25ml @14:04:22.59 Vacuum too high -- waiting up to 40 seconds @14:04:22.60 SS.reconfigure :exhale @14:04:22.65 SS.hold @14:04:23.33 Intake @12.3psia, vacuum from 6.8 to 9.8psi @14:04:23.34 SS.reconfigure :inhaling 1.76ml/s @14:04:23.39 SS.seek 25ml @14:04:26.59 Stroke completed -- waiting for pressure to equalize ... @14:04:26.60 SS.reconfigure :exhale @14:04:26.65 SS.hold @14:04:30.35 Intake.select :close @14:04:30.50 SSV.dial :exhaust @14:04:31.25 Exhaust.select :open @14:04:31.37 SS.seek 0ml @14:04:40.16 Exhaust.select :close @14:04:40.27 SSV.dial CBV @14:04:40.29
CBV.dial SSV @14:04:41.05 Allowing 130s for 25.0ml of remaining 25.0ml @14:04:41.06 Intake.select :open @14:04:43.22 Intake @16.0psia, vacuum from 10.0 to 13.0psi @14:04:43.24 SS.reconfigure :inhaling 1.76ml/s @14:04:43.29 SS.seek 25ml @14:04:55.74 Vacuum too high -- waiting up to 40 seconds @14:04:55.76 SS.reconfigure :exhale @14:04:55.81 SS.hold @14:04:57.40 Intake @13.6psia, vacuum from 8.1 to 11.1psi @14:04:57.42 SS.reconfigure :inhaling 1.41ml/s @14:04:57.47 SS.seek 25ml @14:05:04.37 Stroke completed -- waiting for pressure to equalize ... @14:05:04.39 SS.reconfigure :exhale @14:05:04.43 SS.hold @14:05:09.28 Intake.select :close @14:05:09.44 SSV.dial :exhaust @14:05:10.19 Exhaust.select :open @14:05:10.30 SS.seek 0ml @14:05:19.16 Exhaust.select :close @14:05:19.27 Sampled 50.0ml @14:05:19.28 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @14:05:19.29 Intake.select :close @14:05:19.36 CSV.dial :waste @14:05:19.49 SSV.dial CBV,avoiding: :exhaust @14:05:19.52
CBV.dial SSV @14:05:19.64 CS.seek 0ml @14:05:19.92 CSV.dial :air @14:05:20.67 CS.seek 10ml @14:05:21.43
CTV.dial :air @14:05:22.18 delay 15 seconds @14:05:37.20 SSV.dial :spit,avoiding: :exhaust @14:05:38.52 CTV.dial CSV,avoiding: :intake @14:05:38.56
SS.seek 0ml @14:05:38.68 SSV.dial CBV,avoiding: :exhaust @14:05:45.24 SSV.dial between CBV and plug @14:05:45.72 CBV.dial between SSV and waste2 @14:05:46.12 CTV.dial CSV,avoiding: :air @14:05:46.15
CSV.dial CTV @14:05:46.74 CS.reconfigure :pressurize @14:05:46.79 CS.seek 0ml @14:06:05.03 BubblePoint: Intake @24.2psia, Outlet @0.8psia @14:06:05.04 CBV.dial SSV @14:06:05.37 SS.reconfigure :default @14:06:05.41 SS.seek 10ml @14:06:11.53 delay 15 seconds @14:06:26.66 Evacuated: Intake @23.8psia, Outlet @23.9psia @14:06:26.67 SSV.dial :spit,avoiding: :exhaust @14:06:27.69 SS.reconfigure :exhale @14:06:27.74 SS.seek 0ml @14:06:32.77 SS.reconfigure :fast @14:06:32.81 SS.seek 5ml @14:06:35.66 SSV.dial CBV,avoiding: :exhaust @14:06:35.69
CBV.dial SSV @14:06:37.00 CTV.dial :air,avoiding: :intake @14:06:38.34 CSV.dial CTV @14:06:38.37
SS.seek 10ml @14:06:39.50 CSV.dial :air @14:06:39.94 CS.reconfigure :fast @14:06:39.99 CS.seek 0ml @14:06:40.91
SSV.dial :spit,avoiding: :exhaust @14:06:42.22 SS.reconfigure :exhale @14:06:42.27 SS.seek 0ml @14:06:47.57 SS.reconfigure :fast @14:06:47.62 SS.seek 5ml @14:06:48.02 CS.stop @14:06:50.46 SSV.dial CBV,avoiding: :exhaust @14:06:50.49
CBV.dial SSV @14:06:52.81 SSV.dial :spit,avoiding: :exhaust @14:06:54.13 SS.reconfigure :default @14:06:54.18 SS.seek 0ml @14:06:57.47 SS.stop @14:06:57.51 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @14:06:57.52 Intake.select :close @14:06:57.59 CSV.dial :waste @14:06:57.72 SSV.dial CBV,avoiding: :exhaust @14:06:57.75
CBV.dial SSV @14:06:58.37 CS.seek 0ml @14:06:58.43 CSV.dial :air @14:06:59.08
CTV.dial :air @14:06:59.18 delay 15 seconds @14:06:59.20 CS.seek 10ml @14:07:14.21
SSV.dial :spit,avoiding: :exhaust @14:07:15.54 CTV.dial CSV,avoiding: :intake @14:07:15.56
SS.reconfigure :exhale @14:07:15.67 SS.seek 0ml @14:07:16.05 SSV.dial CBV,avoiding: :exhaust @14:07:23.68 SSV.dial between CBV and plug @14:07:24.14 CBV.dial between SSV and waste2 @14:07:24.53 CSV.dial between air and CTV @14:07:24.84 CS.seek 8.127ml @14:07:29.99 CTV.dial CSV,avoiding: :air @14:07:30.02
CSV.dial CTV @14:07:30.37 CS.reconfigure :pressurize @14:07:30.41 CS.seek 0ml @14:07:48.12 BubblePoint: Intake @24.1psia, Outlet @2.2psia @14:07:48.49 CBV.dial SSV @14:07:48.88 SS.reconfigure :default @14:07:48.93 SS.seek 10ml @14:07:54.53 delay 15 seconds @14:08:09.66 Evacuated: Intake @23.8psia, Outlet @23.4psia @14:08:09.67 SSV.dial :spit,avoiding: :exhaust @14:08:10.69 SS.reconfigure :exhale @14:08:10.74 SS.seek 0ml @14:08:15.76 SS.reconfigure :fast @14:08:15.81 SS.seek 5ml @14:08:18.68 SSV.dial CBV,avoiding: :exhaust @14:08:18.70
CBV.dial SSV @14:08:20.06 CTV.dial :air,avoiding: :intake @14:08:21.40 CSV.dial CTV @14:08:21.44
SS.seek 10ml @14:08:22.65 CSV.dial :air @14:08:23.08 CS.reconfigure :fast @14:08:23.13 CS.seek 0ml @14:08:23.95
SSV.dial :spit,avoiding: :exhaust @14:08:25.27 SS.reconfigure :exhale @14:08:25.32 SS.seek 0ml @14:08:27.23 CS.stop @14:08:30.66
SS.reconfigure :fast @14:08:30.71 SS.seek 5ml @14:08:33.55 SSV.dial CBV,avoiding: :exhaust @14:08:33.58
CBV.dial SSV @14:08:35.88 SSV.dial :spit,avoiding: :exhaust @14:08:37.21 SS.reconfigure :default @14:08:37.25 SS.seek 0ml @14:08:40.54 SS.stop @14:08:40.58 Lysing: Adding 2.0ml AqMeOH @14:08:40.59 CBV.dial between CSV and waste1,avoiding: :DBV @14:08:42.06 CTV.dial CSV @14:08:43.36 CSV.dial CSR @14:08:44.15 Pulling 1ml of CSR.AqMeOH @14:08:44.16 CSR.select :AqMeOH @14:08:44.27 CS.reconfigure :default @14:08:44.34 CS.seek 1ml @14:08:47.77 delay 5 seconds @14:08:52.79 CSR.select :air @14:08:52.96 CS.seek 6ml @14:09:06.80 delay 2 seconds @14:09:08.81 CSV.dial :waste2 @14:09:09.25 CS.reconfigure :fast @14:09:09.30 CS.seek 0ml @14:09:23.67 CSV.dial CSR @14:09:24.12 Pulling 2ml of CSR.AqMeOH @14:09:24.13 CSR.select :AqMeOH @14:09:24.25 CS.reconfigure :default @14:09:24.29 CS.seek 2ml @14:09:30.30 delay 5 seconds @14:09:35.32 CSR.select :air @14:09:35.48 CS.seek 5ml @14:09:44.17 delay 2 seconds @14:09:46.19 CSV.dial CTV @14:09:46.63 CS.seek 1ml @14:09:58.28 Before lysing Collection Puck Heater at 21.9C/27.2psia @14:09:58.30 Heating Puck.from 2, :sh1 in Collection Clamp to 70C for 10:00 @14:09:58.32 CH.seek 70C,8:00 @14:11:52.66 CH.hold 10:00,70C @14:11:52.85 Begun lysing Collection Puck Heater at 68.1C/32.5psia @14:21:52.86 Ended lysing Collection Puck Heater at 70C/31.2psia @14:21:52.88 CS.seek 0ml @14:21:56.29 CTV.dial between spare and air,avoiding: :intake @14:21:57.77 CSV.dial :air @14:21:58.21 CS.seek 5ml @14:22:11.81 CSV.dial CTV @14:22:12.27 delay 2 seconds @14:22:14.29 CSV.dial :air @14:22:14.74 delay 2 seconds @14:22:16.75 CSV.dial CTV @14:22:17.21 delay 2 seconds @14:22:19.22 CSV.dial :air @14:22:19.67 delay 2 seconds @14:22:21.70 CS.seek 0ml @14:22:35.37 Puck @65.2psia after final lysis pressurization @14:22:35.38 Recovering Lysate into Collection syringe @14:22:35.39 CSV.dial CBV @14:22:36.38 CBV.dial CSV @14:22:36.75 CS.seek 5ml @14:22:50.35 delay 5 seconds @14:22:55.36 CTV.dial :air @14:22:55.88 CS.seek 7.001ml @14:23:02.00 delay 5 seconds @14:23:07.02 CSV.dial :air2 @14:23:07.46 CS.seek 10ml @14:23:16.00 CSV.dial between air and CTV,avoiding: CBV @14:23:17.15 Lysing Complete @14:23:17.21 Storing sh1 puck in Collection Clamp to tube 1 @14:23:17.39 SC.reconfigure :tight @14:23:17.44 SC.seek 1 @14:23:21.12 SC.configure :limp @14:23:21.29 SE.reconfigure :place @14:23:21.37
Elbow.reconfigure :tight @14:23:21.41 SE.seek :beamBreak @14:23:21.43
Elbow.seek CC @14:23:21.75 Elbow.reconfigure :loose @14:23:21.85 Forearm.seek CC @14:23:22.88 Hand.close @14:23:23.46 CC.seek :open @14:23:24.29 SE.configure :default @14:23:24.34 SE.seek :place @14:23:28.20
Forearm.seek :retract @14:23:29.24 Elbow.reconfigure :tight @14:23:29.29 Elbow.seek 1 @14:23:31.00 Elbow.reconfigure :limp @14:23:31.05 Forearm.seek 1 @14:23:32.49 Elbow.hold @14:23:32.56 Elbow.reconfigure :tight @14:23:32.61 Hand.open @14:23:33.34 Forearm.force -20 @14:23:33.38 Forearm.seek 1 + 309 counts @14:23:33.68 Forearm.reconfigure :tight @14:23:33.73 Forearm.seek 1 + 450 counts @14:23:33.98 SE.seek :down @14:23:35.11
Forearm.reconfigure :loose @14:23:35.16 Forearm.seek :retract @14:23:36.70 SE.stop @14:23:36.75
Tranferring DA @14:23:36.76 Beginning samplePassC @14:23:36.77 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:23:36.96 Elbow.seek FlushPuck::Garage @14:23:38.75 Elbow.reconfigure :loose @14:23:38.85 Forearm.seek :garage @14:23:39.89 Hand.close @14:23:40.58 Forearm.seek :retract @14:23:41.62 Elbow.reconfigure :tight @14:23:41.67 Elbow.seek CC @14:23:44.63 Elbow.reconfigure :loose @14:23:44.73 Forearm.reconfigure :tight @14:23:44.78 Forearm.seek CC @14:23:45.98 CC.seek :closed @14:23:50.69 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.26 @14:23:50.76 Hand.open @14:23:51.40 Forearm.reconfigure :loose @14:23:51.45 Forearm.seek :retract @14:23:52.38 PSV.dial PSR @14:23:53.76 Pulling 1.5ml of PSR.flush @14:23:53.77 PSR.select :flush @14:23:53.89 PS.seek 1.5ml @14:24:10.01 delay 5 seconds @14:24:15.03 PSV.dial :waste1 @14:24:15.50 PS.seek 0ml @14:24:31.70 PSV.dial PSR @14:24:32.17 PSR.select :air @14:24:32.29 PS.seek 2.5ml @14:24:58.64 PRV.dial CBV,avoiding: :ab2 @14:24:58.68
CBV.dial PRV @14:25:00.39 PSV.dial :mixloop @14:25:01.53 CTV.dial :air,avoiding: :intake @14:25:01.61 PTV.dial :PRVmixing @14:25:02.91 PS.seek 0ml @14:25:29.29 PSV.dial :air @14:25:29.77 PS.seek 2.5ml @14:25:56.10 PSV.dial :mixloop @14:25:56.58 PS.seek 0ml @14:26:22.98 PSV.dial :air @14:26:23.56 PS.seek 2.5ml @14:26:49.91 PSV.dial :mixloop @14:26:50.37 PS.seek 0ml @14:27:16.73 Garaging FlushPuck in Collection Clamp @14:27:16.80 Elbow.reconfigure :tight @14:27:16.85 Elbow.seek CC @14:27:17.17 Elbow.reconfigure :loose @14:27:17.27 Forearm.seek CC @14:27:18.30 Hand.close @14:27:18.96 CC.seek :open @14:27:23.61 Forearm.seek :retract @14:27:24.60 Elbow.reconfigure :tight @14:27:24.65 Elbow.seek :garage @14:27:27.50 Elbow.reconfigure :loose @14:27:28.05 Forearm.reconfigure :tight @14:27:28.10 Forearm.seek :garage @14:27:29.18 Hand.open @14:27:29.89 Forearm.reconfigure :loose @14:27:29.94 Forearm.seek :retract @14:27:30.93 PSV.dial :mixloop @14:27:31.01 PTV.dial :PRVmixing @14:27:31.09 PRV.dial CBV @14:27:31.12
CBV.dial PRV @14:27:31.32 CTV.dial :air,avoiding: :intake @14:27:31.41 Loading lyfil puck from tube 2 into Collection Clamp @14:27:31.64 SC.reconfigure :tight @14:27:31.69 SC.seek 2 @14:27:35.33 SC.configure :limp @14:27:35.54 Elbow.reconfigure :tight @14:27:35.59 Elbow.seek 2 @14:27:37.61 Forearm.reconfigure :tight @14:27:37.66 Forearm.seek 2 + 450 counts @14:27:38.74 SE.reconfigure :pick @14:27:38.80 SE.seek :beamBreak @14:27:41.74 SE.configure :default @14:27:41.90 Elbow.seek 2 @14:27:42.08 Forearm.seek 2 + 300 counts @14:27:42.28 Forearm.reconfigure :loose @14:27:42.33 Hand.close @14:27:42.97 SE.seek :down @14:27:43.19
Forearm.seek :retract @14:27:44.19 Elbow.seek CC @14:27:45.70 SE.stop @14:27:45.86
Elbow.reconfigure :loose @14:27:45.97 Forearm.reconfigure :tight @14:27:46.02 Forearm.seek CC @14:27:47.25 CC.seek :closed @14:27:51.77 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.14 @14:27:51.78 Hand.open @14:27:52.40 Forearm.reconfigure :loose @14:27:52.44 Forearm.seek :retract @14:27:53.40 CTV.dial CSV,avoiding: :intake @14:27:54.75 CSV.dial CTV @14:27:55.07 PS.seek 2.5ml @14:28:21.57 CS.seek 4.501ml @14:28:36.65 delay 8 seconds @14:28:44.67 PSV.dial :air @14:28:45.13 delay 2 seconds @14:28:47.15 PSV.dial :waste2 @14:28:47.89 PS.seek 0ml @14:29:14.22 CBV.dial between PRV and waste1 @14:29:14.56 CSV.dial :air @14:29:15.02 CS.seek 10ml @14:29:29.93 CSV.dial CTV @14:29:30.39 CS.seek 0ml @14:29:56.77 CBV.dial :waste1 @14:29:57.09 delay 5 seconds @14:30:02.11 CTV.dial :air,avoiding: :intake @14:30:03.51 Storing lyfil puck in Collection Clamp to tube 1 @14:30:03.69 SC.reconfigure :tight @14:30:03.73 SC.seek 1 @14:30:07.76 SC.configure :limp @14:30:08.08 Elbow.reconfigure :tight @14:30:08.13 Elbow.seek 1 @14:30:09.89 Forearm.reconfigure :tight @14:30:09.93 Forearm.seek 1 + 450 counts @14:30:10.97 SE.reconfigure :pick @14:30:11.03 SE.seek :beamBreak @14:30:14.06 SE.configure :default @14:30:14.23 Elbow.reconfigure :limp @14:30:14.28 Forearm.reconfigure :loose @14:30:14.33 Forearm.seek 1 @14:30:14.55 Elbow.hold @14:30:14.61 Elbow.reconfigure :tight @14:30:14.71 SE.seek 0.16 Pucks below Top Plate @14:30:15.15 Forearm.force -20 @14:30:15.20 Forearm.seek 1 + 315 counts @14:30:15.48 Forearm.reconfigure :tight @14:30:15.53 Forearm.seek 1 + 450 counts @14:30:15.74 SE.seek 1.16 Pucks below Top Plate @14:30:16.26 Forearm.reconfigure :loose @14:30:16.31 Forearm.seek :retract @14:30:17.22 Elbow.seek CC @14:30:18.89 Elbow.reconfigure :loose @14:30:18.99 Forearm.seek CC @14:30:19.95 Hand.close @14:30:20.58 CC.seek :open @14:30:25.76 Forearm.seek :retract @14:30:26.74 Elbow.reconfigure :tight @14:30:26.79 Elbow.seek 1 @14:30:28.54 Elbow.reconfigure :limp @14:30:28.59 Forearm.seek 1 @14:30:29.66 Elbow.hold @14:30:29.73 Elbow.reconfigure :tight @14:30:29.77 Hand.open @14:30:30.45 Forearm.force -20 @14:30:30.49 Forearm.seek 1 + 317 counts @14:30:30.78 Forearm.reconfigure :tight @14:30:30.82 Forearm.seek 1 + 450 counts @14:30:31.08 SE.seek :down @14:30:32.22
Forearm.reconfigure :loose @14:30:32.27 Forearm.seek :retract @14:30:33.71 SE.stop @14:30:33.75
PSV.dial :air @14:30:34.51 PS.seek 2.5ml @14:31:00.94 delay 1 second @14:31:01.96 PSV.dial :mixloop @14:31:02.52 delay 1 second @14:31:03.54 PSV.dial :air @14:31:03.99 delay 1 second @14:31:05.00 PSV.dial :mixloop @14:31:05.47 delay 1 second @14:31:06.49 PSV.dial :air @14:31:07.07 delay 1 second @14:31:08.09 PS.seek 0ml @14:31:34.63 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @14:31:34.81 Elbow.seek FlushPuck::Garage @14:31:36.63 Elbow.reconfigure :loose @14:31:36.73 Forearm.seek :garage @14:31:37.75 Hand.close @14:31:38.51 Forearm.seek :retract @14:31:39.50 Elbow.reconfigure :tight @14:31:39.55 Elbow.seek PC @14:31:40.82 Elbow.reconfigure :loose @14:31:40.93 Forearm.reconfigure :tight @14:31:40.97 Forearm.seek PC @14:31:42.16 PC.seek :closed @14:31:46.87 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.60 @14:31:46.94 Hand.open @14:31:47.64 Forearm.reconfigure :loose @14:31:47.68 Forearm.seek :retract @14:31:48.61 PRV.dial :air @14:31:49.07 PSV.dial :mixloop @14:31:49.58 PS.seek 0.3ml @14:31:53.50 PSV.dial PSR @14:31:54.66 Pulling 0.4ml of PSR.air @14:31:54.67 PSR.select :air @14:31:54.69 PS.seek 0.7ml @14:31:59.63 delay 3 seconds @14:32:02.64 PSR.select :air @14:32:02.67 PS.seek 2.5ml @14:32:21.88 delay 3 seconds @14:32:24.89 PSV.dial :mixloop @14:32:26.03 PTV.dial :mixing @14:32:26.78 PBV.dial :waste2 @14:32:27.73 PS.seek 0ml @14:32:54.10 Garaging FlushPuck in Processing Clamp @14:32:54.17 Elbow.reconfigure :tight @14:32:54.22 Elbow.seek PC @14:32:54.34 Elbow.reconfigure :loose @14:32:54.91 Forearm.seek PC @14:32:55.91 Hand.close @14:32:56.58 PC.seek :open @14:33:01.31 Forearm.seek :retract @14:33:02.27 Elbow.reconfigure :tight @14:33:02.32 Elbow.seek :garage @14:33:03.70 Elbow.reconfigure :loose @14:33:03.80 Forearm.reconfigure :tight @14:33:03.85 Forearm.seek :garage @14:33:04.92 Hand.open @14:33:05.64 Forearm.reconfigure :loose @14:33:05.69 Forearm.seek :retract @14:33:06.72 Loading da puck from tube 2 into Processing Clamp @14:33:06.96 SC.reconfigure :tight @14:33:07.01 SC.seek 2 @14:33:10.60 SC.configure :limp @14:33:10.82 Elbow.reconfigure :tight @14:33:10.87 Elbow.seek 2 @14:33:12.71 Forearm.reconfigure :tight @14:33:12.76 Forearm.seek 2 + 450 counts @14:33:13.79 SE.reconfigure :pick @14:33:13.85 SE.seek :beamBreak @14:33:16.87 SE.configure :default @14:33:17.03 Elbow.seek 2 @14:33:17.22 Forearm.seek 2 + 300 counts @14:33:17.44 Forearm.reconfigure :loose @14:33:17.48 Hand.close @14:33:18.10 SE.seek :down @14:33:18.68
Forearm.seek :retract @14:33:19.70 Elbow.seek PC @14:33:20.81 SE.stop @14:33:22.23
Elbow.reconfigure :loose @14:33:22.33 Forearm.reconfigure :tight @14:33:22.38 Forearm.seek PC @14:33:23.65 PC.seek :closed @14:33:28.31 Processing Clamp onSpring/offSpring for 16/26 samples, on/off torque 1.62 @14:33:28.32 Hand.open @14:33:29.01 Forearm.reconfigure :loose @14:33:29.06 Forearm.seek :retract @14:33:30.02 Heating Puck.from 2, :da in Processing Clamp to 28C for 2:00:00 @14:33:30.04 PH.seek 28C,5:00 @14:34:01.18 PH.hold 2:00:00,28C @14:34:01.23 delay 1:30 @14:35:31.28 PSR.select :air @14:35:31.29 PSV.dial PSR @14:35:32.33 Pulling 0.5ml of PSR.wash @14:35:32.34 PSR.select :wash @14:35:32.46 PS.seek 0.5ml @14:35:38.40 delay 5 seconds @14:35:43.42 PSR.select :air @14:35:43.55 PS.seek 2ml @14:35:59.68 PTV.dial :puck @14:36:00.42 PSV.dial PTV @14:36:01.29 PBV.dial :waste1 @14:36:02.66 PS.seek 1.5ml @14:36:08.69 delay 3 seconds @14:36:11.70 PBV.dial between PSV and waste1 @14:36:12.07 PS.seek 0ml @14:36:28.28 delay 5:00 @14:41:28.31 recoverPSRreagent08 @14:41:28.32 PTV.dial :puck @14:41:28.40 PBV.dial :waste1 @14:41:28.78 delay 30 seconds @14:41:58.80 PBV.dial between PSV and waste1 @14:41:59.16 PSR.select :air @14:41:59.18 PSV.dial PSR @14:41:59.92 PS.reconfigure :fast @14:41:59.97 PS.seek 2.5ml @14:42:23.64 PSV.dial PTV @14:42:24.40 delay 5 seconds @14:42:29.42 PSV.dial PSR @14:42:30.18 delay 5 seconds @14:42:35.20 PSV.dial PTV @14:42:35.95 delay 5 seconds @14:42:40.96 PSV.dial :air @14:42:41.72 PS.seek 0ml @14:43:05.02 PTV.dial :PRVmixing,via: PRV @14:43:06.32 PSV.dial :mixloop @14:43:06.79 PRV.dial :ab1 @14:43:07.53 PS.reconfigure :slow @14:43:07.57 PS.seek 0.15ml @14:43:11.46 delay 2 seconds @14:43:13.48 CBV.dial PRV @14:43:13.51
PRV.dial CBV @14:43:14.06 PS.seek 0.34ml @14:43:18.43 delay 2 seconds @14:43:20.44 CBV.dial between PRV and PSV @14:43:20.76 PRV.dial :air @14:43:21.21 PS.seek 0.9ml @14:43:33.51 PRV.dial :waste2 @14:43:34.31 PS.seek 0.5ml @14:43:42.96 addPMLReagent08 @14:43:42.97 PSV.dial :air @14:43:43.43 PS.reconfigure :default @14:43:43.48 PS.seek 2ml @14:43:59.58 PSV.dial :PML @14:44:00.05 PTV.dial :mixing @14:44:00.80 PBV.dial :waste1 @14:44:01.24 PS.seek 1.85ml @14:44:03.67 PBV.dial between PSV and waste1 @14:44:04.04 PS.seek 0ml @14:44:23.77 delay 2 seconds @14:44:25.79 Beginning samplePassC @14:44:25.80 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:44:25.99 Elbow.reconfigure :tight @14:44:26.03 Elbow.seek FlushPuck::Garage @14:44:27.41 Elbow.reconfigure :loose @14:44:27.51 Forearm.seek :garage @14:44:28.52 Hand.close @14:44:29.32 Forearm.seek :retract @14:44:30.29 Elbow.reconfigure :tight @14:44:30.34 Elbow.seek CC @14:44:33.29 Elbow.reconfigure :loose @14:44:33.39 Forearm.reconfigure :tight @14:44:33.44 Forearm.seek CC @14:44:34.65 CC.seek :closed @14:44:39.62 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.38 @14:44:39.69 Hand.open @14:44:40.38 Forearm.reconfigure :loose @14:44:40.43 Forearm.seek :retract @14:44:41.38 PSV.dial PSR @14:44:42.55 Pulling 1.5ml of PSR.flush @14:44:42.56 PSR.select :flush @14:44:42.68 PS.seek 1.5ml @14:44:58.89 delay 5 seconds @14:45:03.91 PSV.dial :waste1 @14:45:04.40 PS.seek 0ml @14:45:20.58 PSV.dial PSR @14:45:21.05 PSR.select :air @14:45:21.17 PS.seek 2.5ml @14:45:47.62 PRV.dial CBV,avoiding: :ab2 @14:45:47.65
CBV.dial PRV @14:45:49.24 PSV.dial :mixloop @14:45:50.27 CTV.dial :air,avoiding: :intake @14:45:50.45 PTV.dial :PRVmixing @14:45:51.19 PS.seek 0ml @14:46:17.61 PSV.dial :air @14:46:18.09 PS.seek 2.5ml @14:46:44.45 PSV.dial :mixloop @14:46:44.93 PS.seek 0ml @14:47:11.29 PSV.dial :air @14:47:11.77 PS.seek 2.5ml @14:47:38.08 PSV.dial :mixloop @14:47:38.55 PS.seek 0ml @14:48:05.00 Garaging FlushPuck in Collection Clamp @14:48:05.07 Elbow.reconfigure :tight @14:48:05.12 Elbow.seek CC @14:48:05.43 Elbow.reconfigure :loose @14:48:05.53 Forearm.seek CC @14:48:06.49 Hand.close @14:48:07.07 CC.seek :open @14:48:11.72 Forearm.seek :retract @14:48:12.74 Elbow.reconfigure :tight @14:48:12.79 Elbow.seek :garage @14:48:16.07 Elbow.reconfigure :loose @14:48:16.18 Forearm.reconfigure :tight @14:48:16.22 Forearm.seek :garage @14:48:17.28 Hand.open @14:48:18.00 Forearm.reconfigure :loose @14:48:18.05 Forearm.seek :retract @14:48:19.04 delayUntil "14:52:25.78" @14:48:19.06 Whole Cell Archive sampling 50ml @14:48:19.08 Loading wcr puck from tube 2 into Collection Clamp @14:48:19.33 SC.reconfigure :tight @14:48:19.38 SC.seek 2 @14:48:19.70 SC.configure :limp @14:48:19.93 Elbow.reconfigure :tight @14:48:19.98 Elbow.seek 2 @14:48:21.97 Forearm.reconfigure :tight @14:48:22.02 Forearm.seek 2 + 450 counts @14:48:23.07 SE.reconfigure :pick @14:48:23.13 SE.seek :beamBreak @14:48:26.19 SE.configure :default @14:48:26.35 Elbow.seek 2 @14:48:26.55 Forearm.seek 2 + 300 counts @14:48:26.74 Forearm.reconfigure :loose @14:48:26.79 Hand.close @14:48:27.45 Puck::Warning in WCR -- Loaded Last remaining Puck (from Tube 2) @14:48:27.47 SE.seek :down @14:48:27.68 Forearm.seek :retract @14:48:28.60 Elbow.seek CC @14:48:30.64 Elbow.reconfigure :loose @14:48:30.74 SE.stop @14:48:30.86 Forearm.reconfigure :tight @14:48:30.91 Forearm.seek CC @14:48:32.16 CC.seek :closed @14:48:36.68 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.29 @14:48:36.70 Hand.open @14:48:37.37 Forearm.reconfigure :loose @14:48:37.43 Forearm.seek :retract @14:48:38.40 collectSample 50ml w/bubble point of 35psi @14:48:38.41 Priming 7ml @14:48:38.42 Intake.select :close @14:48:38.43 Exhaust.select :close @14:48:38.44 SS.hold @14:48:38.66 Calibrating pressure sensors @14:48:38.67 CTV.dial :air @14:48:38.69 SSV.dial :spit,avoiding: :exhaust @14:48:38.77 SS.reconfigure :fast @14:48:38.82 SS.seek 5ml @14:48:41.56 SSV.dial CBV @14:48:41.59 CBV.dial SSV @14:48:42.97 delay 3.8 seconds @14:48:46.90 Sensor limits: Intake max 116.3psia; Outlet max 111.0psia @14:48:46.91 SSV.dial :spit @14:48:48.24 SS.seek 0ml @14:48:51.28 SSV.dial CBV @14:48:52.61 CTV.dial :intake @14:48:53.37 SSV.dial CBV @14:48:53.40 CBV.dial SSV @14:48:53.60 Allowing 40s for 7.0ml of remaining 7.0ml @14:48:53.62 Intake.select :open @14:48:55.79 Intake @15.6psia, vacuum from 10.0 to 13.0psi @14:48:55.81 SS.reconfigure :inhaling 2.20ml/s @14:48:56.40 SS.seek 7ml @14:49:01.88 Stroke completed -- waiting for pressure to equalize ... @14:49:01.90 SS.reconfigure :exhale @14:49:01.95 SS.hold @14:49:06.81 Intake.select :close @14:49:06.98 SSV.dial :exhaust @14:49:07.76 Exhaust.select :open @14:49:07.88 SS.seek 0ml @14:49:11.93 Exhaust.select :close @14:49:12.05 Sampling 50.0ml @14:49:12.07 Intake.select :close @14:49:12.08 Exhaust.select :close @14:49:12.09 SS.reconfigure :default @14:49:12.14 SS.hold @14:49:12.35 CTV.dial :intake @14:49:12.53 SSV.dial CBV @14:49:12.55 CBV.dial SSV @14:49:13.34 Allowing 130s for 25.0ml of remaining 50.0ml @14:49:13.35 Intake.select :open @14:49:15.52 Intake @16.1psia, vacuum from 10.0 to 13.0psi @14:49:15.54 SS.reconfigure :inhaling 2.20ml/s @14:49:15.59 SS.seek 25ml @14:49:24.94 Vacuum too high -- waiting up to 40 seconds @14:49:24.95 SS.reconfigure :exhale @14:49:25.01 SS.hold @14:49:26.47 Intake @13.6psia, vacuum from 8.1 to 11.1psi @14:49:26.49 SS.reconfigure :inhaling 1.76ml/s @14:49:26.54 SS.seek 25ml @14:49:34.59 Stroke completed -- waiting for pressure to equalize ... @14:49:34.60 SS.reconfigure :exhale @14:49:34.65 SS.hold @14:49:39.54 Intake.select :close @14:49:39.71 SSV.dial :exhaust @14:49:40.46 Exhaust.select :open @14:49:40.58 SS.seek 0ml @14:49:49.41 Exhaust.select :close @14:49:49.53 SSV.dial CBV @14:49:49.56 CBV.dial SSV @14:49:50.34 Allowing 130s for 25.0ml of remaining 25.0ml @14:49:50.35 Intake.select :open @14:49:52.51 Intake @16.1psia, vacuum from 10.0 to 13.0psi @14:49:52.53 SS.reconfigure :inhaling 1.76ml/s @14:49:52.58 SS.seek 25ml @14:50:01.97 Vacuum too high -- waiting up to 40 seconds @14:50:01.98 SS.reconfigure :exhale @14:50:02.04 SS.hold @14:50:04.51 Intake @14.6psia, vacuum from 9.1 to 12.1psi @14:50:04.53 SS.reconfigure :inhaling 1.41ml/s @14:50:04.58 SS.seek 25ml @14:50:15.32 Stroke completed -- waiting for pressure to equalize ... @14:50:15.33 SS.reconfigure :exhale @14:50:15.38 SS.hold @14:50:21.93 Intake.select :close @14:50:22.09 SSV.dial :exhaust @14:50:22.85 Exhaust.select :open @14:50:22.97 SS.seek 0ml @14:50:32.05 Exhaust.select :close @14:50:32.17 Sampled 50.0ml @14:50:32.19 Drying puck before fixing for 4:00 @14:50:32.20 CBV.dial :waste2b @14:50:32.96 delay 1:00 @14:51:32.99 CSV.dial :air @14:51:33.45 CS.reconfigure :fast @14:51:33.50 CS.seek 10ml @14:51:58.32 Pumping wcr puck from tube 2 up to 35psi... @14:51:58.34 CBV.dial SSV @14:51:59.60 CBV.dial between CSV and waste2b @14:52:01.00 CSV.dial between air and CTV @14:52:01.32 CS.seek 7.292ml @14:52:08.35 CTV.dial CSV,avoiding: :air @14:52:08.38 CSV.dial CTV @14:52:09.18 CS.reconfigure :pressurize @14:52:09.23 CS.seek 0ml @14:52:24.27 CBV.dial :waste2b @14:52:24.60 CS.reconfigure :default @14:52:24.65 CS.seek 0ml @14:52:25.79 recoverPRVreagent08 @14:52:25.80 PTV.dial :mixing @14:52:26.54 PBV.dial :waste1 @14:52:26.90 delay 30 seconds @14:52:29.72 delay 1:00 @14:52:56.92 PBV.dial between PSV and waste1 @14:52:57.29 PSV.dial :air @14:52:57.76 PS.reconfigure :fast @14:52:57.81 PS.seek 2.5ml @14:53:21.76 PSV.dial :mixloop @14:53:22.23 delay 2 seconds @14:53:24.25 PSV.dial :air @14:53:24.71 delay 2 seconds @14:53:26.73 PSV.dial :mixloop @14:53:27.20 delay 2 seconds @14:53:29.21 PSV.dial :air @14:53:29.74 CTV.dial :air,avoiding: :intake @14:53:29.82 PS.seek 0ml @14:53:31.09 Fix #1 of 1 (with RNAlater) for 4:00 @14:53:31.10 CSV.dial CSR @14:53:31.53 Pulling 2ml of CSR.RNAlater @14:53:31.54 CSR.select :RNAlater @14:53:31.66 CS.seek 2ml @14:53:37.57 delay 5 seconds @14:53:42.59 CSR.select :air @14:53:42.71 CS.reconfigure :fast @14:53:42.76 CS.seek 10ml @14:53:53.29 washCycleDA #1 of 2 @14:53:53.30 PSR.select :air @14:53:53.32 PSV.dial PSR @14:53:54.65 Pulling 0.5ml of PSR.wash @14:53:54.66 PSR.select :wash @14:53:54.78 PS.reconfigure :default @14:53:54.83 PS.seek 0.5ml @14:54:00.82 delay 5 seconds @14:54:02.69 Pumping wcr puck from tube 2 up to 35psi... @14:54:02.71 CBV.dial SSV @14:54:03.96 CBV.dial between CSV and waste2b @14:54:04.92 CSV.dial between air and CTV @14:54:05.83 PSR.select :air @14:54:05.95 CS.seek 7.326ml @14:54:06.02 PS.seek 2ml @14:54:12.94 CTV.dial CSV,avoiding: :air @14:54:12.98 CSV.dial CTV @14:54:14.33 CS.reconfigure :pressurize @14:54:14.39 CS.seek 0ml @14:54:22.19 PTV.dial :puck @14:54:22.93 PSV.dial PTV @14:54:23.69 PBV.dial :waste1 @14:54:24.11 PS.seek 1.5ml @14:54:30.01 delay 3 seconds @14:54:30.74 delay 4:00 @14:54:33.03 PBV.dial between PSV and waste1 @14:54:33.39 PS.seek 0ml @14:54:49.44 delay 2:00 @14:56:49.47 recoverPSRreagent08 @14:56:49.48 PTV.dial :puck @14:56:49.57 PBV.dial :waste1 @14:56:49.93 delay 30 seconds @14:57:19.95 PBV.dial between PSV and waste1 @14:57:20.31 PSR.select :air @14:57:20.33 PSV.dial PSR @14:57:21.10 PS.reconfigure :fast @14:57:21.15 PS.seek 2.5ml @14:57:45.03 PSV.dial PTV @14:57:45.80 delay 5 seconds @14:57:50.82 PSV.dial PSR @14:57:51.57 delay 5 seconds @14:57:56.59 PSV.dial PTV @14:57:57.33 delay 5 seconds @14:58:02.35 PSV.dial :air @14:58:03.24 PS.seek 0ml @14:58:26.72 washCycleDA #2 of 2 @14:58:26.73 PSR.select :air @14:58:26.75 PSV.dial PSR @14:58:28.20 Pulling 0.5ml of PSR.wash @14:58:28.22 PSR.select :wash @14:58:28.33 PS.reconfigure :default @14:58:28.38 PS.seek 0.5ml @14:58:30.76 CBV.dial :waste2b @14:58:31.09 CS.reconfigure :default @14:58:31.15 CS.seek 0ml @14:58:34.38 delay 5 seconds @14:58:34.97 delay 1:00 @14:58:39.40 PSR.select :air @14:58:39.52 PS.seek 2ml @14:58:55.72 PTV.dial :puck @14:58:55.80 PSV.dial PTV @14:58:56.55 PBV.dial :waste1 @14:58:56.97 PS.seek 1.5ml @14:59:03.00 delay 3 seconds @14:59:06.02 PBV.dial between PSV and waste1 @14:59:06.54 PS.seek 0ml @14:59:22.72 delay 2:00 @14:59:34.99 CTV.dial :air,avoiding: :intake @15:01:22.75 recoverPSRreagent08 @15:01:22.76 PTV.dial :puck @15:01:22.83 PBV.dial :waste1 @15:01:23.20 delay 30 seconds @15:01:53.23 PBV.dial between PSV and waste1 @15:01:53.59 PSR.select :air @15:01:53.61 PSV.dial PSR @15:01:54.38 PS.reconfigure :fast @15:01:54.43 PS.seek 2.5ml @15:02:18.34 PSV.dial PTV @15:02:19.11 delay 5 seconds @15:02:24.13 PSV.dial PSR @15:02:24.87 delay 5 seconds @15:02:29.89 PSV.dial PTV @15:02:30.64 delay 5 seconds @15:02:35.66 PSV.dial :air @15:02:36.53 PS.seek 0ml @15:03:00.12 getPMLReagent08 @15:03:00.13 PRV.dial :air @15:03:00.59 delay 5 seconds @15:03:05.61 PTV.dial :PRVmixing @15:03:06.93 delay 5 seconds @15:03:11.96 PRV.dial :ab2 @15:03:12.40 PTV.dial :PRVmixing @15:03:12.59 PSV.dial :mixloop @15:03:13.05 PS.reconfigure :default @15:03:13.09 PS.seek 0.3ml @15:03:16.96 delay 2 seconds @15:03:18.99 PRV.dial :air @15:03:19.51 PS.seek 0.55ml @15:03:22.94 addPMLReagent08 @15:03:22.95 PSV.dial :air @15:03:23.42 PS.seek 2ml @15:03:39.24 PSV.dial :PML @15:03:39.71 PTV.dial :mixing @15:03:40.48 PBV.dial :waste1 @15:03:40.90 PS.seek 1.85ml @15:03:43.31 PBV.dial between PSV and waste1 @15:03:43.67 PS.seek 0ml @15:04:03.40 delay 2 seconds @15:04:05.42 delay 8:00 @15:12:05.45 recoverPRVreagent08 @15:12:05.46 PTV.dial :mixing @15:12:05.53 PBV.dial :waste1 @15:12:05.89 delay 30 seconds @15:12:35.92 PBV.dial between PSV and waste1 @15:12:36.28 PSV.dial :air @15:12:36.75 PS.reconfigure :fast @15:12:36.80 PS.seek 2.5ml @15:13:00.95 PSV.dial :mixloop @15:13:01.42 delay 2 seconds @15:13:03.44 PSV.dial :air @15:13:03.91 delay 2 seconds @15:13:05.92 PSV.dial :mixloop @15:13:06.39 delay 2 seconds @15:13:08.41 PSV.dial :air @15:13:08.88 PS.seek 0ml @15:13:32.77 washCycleDA #1 of 3 @15:13:32.78 PSR.select :air @15:13:32.79 PSV.dial PSR @15:13:34.11 Pulling 0.5ml of PSR.wash @15:13:34.12 PSR.select :wash @15:13:34.24 PS.reconfigure :default @15:13:34.29 PS.seek 0.5ml @15:13:40.26 delay 5 seconds @15:13:45.28 PSR.select :air @15:13:45.41 PS.seek 2ml @15:14:01.57 PTV.dial :puck @15:14:02.31 PSV.dial PTV @15:14:03.06 PBV.dial :waste1 @15:14:03.48 PS.seek 1.5ml @15:14:09.39 delay 3 seconds @15:14:12.41 PBV.dial between PSV and waste1 @15:14:12.79 PS.seek 0ml @15:14:28.95 delay 2:00 @15:16:28.98 recoverPSRreagent08 @15:16:28.99 PTV.dial :puck @15:16:29.06 PBV.dial :waste1 @15:16:29.43 delay 30 seconds @15:16:59.46 PBV.dial between PSV and waste1 @15:16:59.82 PSR.select :air @15:16:59.84 PSV.dial PSR @15:17:00.61 PS.reconfigure :fast @15:17:00.66 PS.seek 2.5ml @15:17:24.76 PSV.dial PTV @15:17:25.53 delay 5 seconds @15:17:30.55 PSV.dial PSR @15:17:31.29 delay 5 seconds @15:17:36.31 PSV.dial PTV @15:17:37.07 delay 5 seconds @15:17:42.09 PSV.dial :air @15:17:42.86 PS.seek 0ml @15:18:06.29 washCycleDA #2 of 3 @15:18:06.30 PSR.select :air @15:18:06.32 PSV.dial PSR @15:18:07.63 Pulling 0.5ml of PSR.wash @15:18:07.64 PSR.select :wash @15:18:07.76 PS.reconfigure :default @15:18:07.81 PS.seek 0.5ml @15:18:13.85 delay 5 seconds @15:18:18.87 PSR.select :air @15:18:18.99 PS.seek 2ml @15:18:35.16 PTV.dial :puck @15:18:35.18 PSV.dial PTV @15:18:35.93 PBV.dial :waste1 @15:18:36.35 PS.seek 1.5ml @15:18:42.35 delay 3 seconds @15:18:45.37 PBV.dial between PSV and waste1 @15:18:45.73 PS.seek 0ml @15:19:01.82 delay 2:00 @15:21:01.85 recoverPSRreagent08 @15:21:01.86 PTV.dial :puck @15:21:01.87 PBV.dial :waste1 @15:21:02.22 delay 30 seconds @15:21:32.25 PBV.dial between PSV and waste1 @15:21:32.61 PSR.select :air @15:21:32.63 PSV.dial PSR @15:21:33.40 PS.reconfigure :fast @15:21:33.45 PS.seek 2.5ml @15:21:57.35 PSV.dial PTV @15:21:58.58 delay 5 seconds @15:22:03.60 PSV.dial PSR @15:22:04.35 delay 5 seconds @15:22:09.37 PSV.dial PTV @15:22:10.15 delay 5 seconds @15:22:15.17 PSV.dial :air @15:22:15.92 PS.seek 0ml @15:22:39.29 washCycleDA #3 of 3 @15:22:39.30 PSR.select :air @15:22:39.31 PSV.dial PSR @15:22:40.64 Pulling 0.5ml of PSR.wash @15:22:40.65 PSR.select :wash @15:22:40.77 PS.reconfigure :default @15:22:40.82 PS.seek 0.5ml @15:22:46.82 delay 5 seconds @15:22:51.84 PSR.select :air @15:22:51.96 PS.seek 2ml @15:23:08.06 PTV.dial :puck @15:23:08.08 PSV.dial PTV @15:23:08.94 PBV.dial :waste1 @15:23:09.36 PS.seek 1.5ml @15:23:15.41 delay 3 seconds @15:23:18.43 PBV.dial between PSV and waste1 @15:23:18.80 PS.seek 0ml @15:23:34.88 delay 2:00 @15:25:34.91 recoverPSRreagent08 @15:25:34.92 PTV.dial :puck @15:25:34.93 PBV.dial :waste1 @15:25:35.30 delay 30 seconds @15:26:05.33 PBV.dial between PSV and waste1 @15:26:05.69 PSR.select :air @15:26:05.71 PSV.dial PSR @15:26:06.44 PS.reconfigure :fast @15:26:06.49 PS.seek 2.5ml @15:26:30.18 PSV.dial PTV @15:26:31.06 delay 5 seconds @15:26:36.08 PSV.dial PSR @15:26:36.81 delay 5 seconds @15:26:41.83 PSV.dial PTV @15:26:42.70 delay 5 seconds @15:26:47.72 PSV.dial :air @15:26:48.49 PS.seek 0ml @15:27:11.84 Gate.power :camera,:ON @15:27:11.86 Mixing DA Substrates PSR.select :air @15:27:11.88 PSV.dial PSR @15:27:13.27 Pulling 0.25ml of PSR.sub1 @15:27:13.31 PSR.select :sub1 @15:27:13.51 PS.reconfigure :default @15:27:13.64 PS.seek 0.25ml @15:27:17.23 delay 5 seconds @15:27:22.25 Pulling 0.25ml of PSR.sub2 @15:27:22.26 PSR.select :sub2 @15:27:22.38 PS.seek 0.5ml @15:27:25.81 delay 5 seconds @15:27:30.83 PSR.select :air @15:27:30.94 PS.seek 2ml @15:27:47.12 PTV.dial :puck @15:27:47.13 PSV.dial PTV @15:27:48.01 PBV.dial :waste1 @15:27:48.45 PS.seek 1.5ml @15:27:54.50 delay 3 seconds @15:27:57.52 PBV.dial between PSV and waste1 @15:27:57.89 PS.seek 0ml @15:28:14.00 delay 10 seconds @15:28:24.02 recoverPSRreagent08 @15:28:24.03 PTV.dial :puck @15:28:24.05 PBV.dial :waste1 @15:28:24.90 delay 30 seconds @15:28:54.93 PBV.dial between PSV and waste1 @15:28:55.31 PSR.select :air @15:28:55.33 PSV.dial PSR @15:28:56.09 PS.reconfigure :fast @15:28:56.14 PS.seek 2.5ml @15:29:20.21 PSV.dial PTV @15:29:21.11 delay 5 seconds @15:29:26.13 PSV.dial PSR @15:29:26.87 delay 5 seconds @15:29:31.89 PSV.dial PTV @15:29:32.78 delay 5 seconds @15:29:37.80 PSV.dial :air @15:29:38.58 PS.seek 0ml @15:30:02.46 Additional air clear @15:30:02.48 PSR.select :air @15:30:02.49 PSV.dial PSR @15:30:03.84 Pulling 2ml of PSR.air @15:30:03.85 PSR.select :air @15:30:03.87 PS.reconfigure :default @15:30:03.92 PS.seek 2ml @15:30:25.27 delay 5 seconds @15:30:30.30 PTV.dial :puck @15:30:30.31 PSV.dial PTV @15:30:31.06 PBV.dial :waste1 @15:30:31.49 PS.seek 1.5ml @15:30:37.49 delay 3 seconds @15:30:40.51 PBV.dial between PSV and waste1 @15:30:40.88 delay 1 second @15:30:41.90 PSV.dial :waste1 @15:30:42.97 PS.seek 0ml @15:30:59.14 Processing Puck Heater at 28.1C before imaging @15:30:59.15 PH.coast @15:30:59.26 Imaging da puck in Processing Clamp while storing it to tube 1 @15:30:59.44 SC.reconfigure :tight @15:30:59.49 SC.seek 1 @15:31:03.14 SC.configure :limp @15:31:03.36 Elbow.reconfigure :tight @15:31:03.42 Elbow.seek 1 @15:31:05.19 Forearm.reconfigure :tight @15:31:05.24 Forearm.seek 1 + 450 counts @15:31:06.28 SE.reconfigure :pick @15:31:06.33 SE.seek :beamBreak @15:31:09.40 SE.configure :default @15:31:09.56 Elbow.reconfigure :limp @15:31:09.61 Forearm.reconfigure :loose @15:31:09.66 Forearm.seek 1 @15:31:09.90 Elbow.hold @15:31:09.97 Elbow.reconfigure :tight @15:31:10.59 SE.seek 1.17 Pucks below Top Plate @15:31:11.02 Forearm.force -20 @15:31:11.07 Forearm.seek 1 + 315 counts @15:31:11.35 Forearm.reconfigure :tight @15:31:11.40 Forearm.seek 1 + 450 counts @15:31:11.60 SE.seek 2.17 Pucks below Top Plate @15:31:12.10 Forearm.reconfigure :loose @15:31:12.15 Forearm.seek :retract @15:31:13.08 Elbow.seek PC @15:31:15.75 Elbow.reconfigure :loose @15:31:15.85 Forearm.seek PC @15:31:16.81 Hand.close @15:31:17.41 PC.seek :open @15:31:22.16 Forearm.seek :retract @15:31:23.14 Elbow.reconfigure :tight @15:31:23.19 Elbow.seek 1 @15:31:25.72 Forearm.reconfigure :tight @15:31:25.77 Forearm.seek 1 + 450 counts @15:31:26.74 Hand.open @15:31:27.47 SE.seek 1.82 Pucks below Top Plate @15:31:27.77 SE.reconfigure :seal @15:31:27.82 SE.seek 0.86 Pucks below Top Plate @15:31:28.35 Lights, camera, ACTION! @15:31:28.39 PuckCamera: snap -bin=2,2 -auto /var/log/esp/da24jan2315h50ml.tif @15:31:47.88 Waiting >20 seconds for da to relinquish FlushPuck @15:32:45.83 SX HX9/16 (USB:07): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 74.04 seconds /var/log/esp/da24jan2315h50ml.tif: TIFF Upload Complete @15:32:45.85 PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da24jan2315h50ml15s.tif @15:33:01.50 SX HX9/16 (USB:07): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15 seconds /var/log/esp/midres/da24jan2315h50ml15s.tif: TIFF Upload Complete @15:33:01.53 PuckCamera: snap -bin=2,2 20.0 /var/log/esp/midres/da24jan2315h50ml20s.tif @15:33:22.14 SX HX9/16 (USB:07): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 20 seconds /var/log/esp/midres/da24jan2315h50ml20s.tif: TIFF Upload Complete @15:33:22.16 Gate.power :camera,:OFF @15:33:22.18 DAfans.email "74.04 sec auto-exposure",Subject: "da24jan2315h50ml images!" @15:33:22.61 Elbow.seek 1 @15:33:22.80 Forearm.seek 1 + 450 counts @15:33:22.97 SE.reconfigure :gently @15:33:23.04 SE.seek 1.57 Pucks below Top Plate @15:33:24.27 Elbow.reconfigure :limp @15:33:24.33 Forearm.reconfigure :loose @15:33:24.38 Forearm.seek 1 @15:33:24.65 Elbow.hold @15:33:24.74 Elbow.reconfigure :tight @15:33:24.79 SE.reconfigure :default @15:33:24.84 SE.seek 1.76 Pucks below Top Plate @15:33:25.25 Forearm.force -20 @15:33:25.29 Forearm.seek 1 + 315 counts @15:33:25.60 Forearm.reconfigure :tight @15:33:25.65 Forearm.seek 1 + 450 counts @15:33:26.19 SE.seek :down @15:33:27.47 Forearm.reconfigure :loose @15:33:27.62 Forearm.seek :retract @15:33:28.85 SE.stop @15:33:29.09 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @15:33:29.77 Elbow.seek FlushPuck::Garage @15:33:31.76 Elbow.reconfigure :loose @15:33:31.96 Forearm.seek :garage @15:33:33.06 Hand.close @15:33:33.94 Forearm.seek :retract @15:33:34.99 Elbow.reconfigure :tight @15:33:35.09 Elbow.seek PC @15:33:36.48 Elbow.reconfigure :loose @15:33:36.75 Forearm.reconfigure :tight @15:33:36.88 Forearm.seek PC @15:33:39.61 PC.seek :closed @15:33:44.47 Processing Clamp onSpring/offSpring for 16/26 samples, on/off torque 1.64 @15:33:44.59 Hand.open @15:33:45.48 Forearm.reconfigure :loose @15:33:45.58 Forearm.seek :retract @15:33:46.59 Storing wcr puck in Collection Clamp to tube 1 @15:33:46.63 PSR.select :air @15:33:46.74 PSV.dial PSR @15:33:47.20 SC.reconfigure :tight @15:33:47.35 SC.seek 1 @15:33:47.46 Pulling 0.5ml of PSR.flush @15:33:47.50 PSR.select :flush @15:33:47.67 PS.seek 0.5ml @15:33:48.34 SC.configure :limp @15:33:48.80 Elbow.reconfigure :tight @15:33:48.90 Elbow.seek 1 @15:33:51.59 upsync @15:33:51.70 Forearm.reconfigure :tight @15:33:51.76 Forearm.seek 1 + 450 counts @15:33:52.86 SE.reconfigure :pick @15:33:52.91 SE.seek :beamBreak @15:33:53.61 delay 5 seconds @15:33:55.87 SE.configure :default @15:33:56.03 Elbow.reconfigure :limp @15:33:56.09 Forearm.reconfigure :loose @15:33:56.13 Forearm.seek 1 @15:33:56.35 Elbow.hold @15:33:56.43 Elbow.reconfigure :tight @15:33:57.00 SE.seek 2.16 Pucks below Top Plate @15:33:57.45 Forearm.force -20 @15:33:57.50 Forearm.seek 1 + 310 counts @15:33:57.90 Forearm.reconfigure :tight @15:33:57.95 Forearm.seek 1 + 450 counts @15:33:58.20 SE.seek 3.16 Pucks below Top Plate @15:33:58.63 PSR.select :air @15:33:58.82 PS.seek 2.5ml @15:33:58.90 Forearm.reconfigure :loose @15:33:58.95 Forearm.seek :retract @15:33:59.87 Elbow.seek CC @15:34:01.55 Elbow.reconfigure :loose @15:34:01.66 Forearm.seek CC @15:34:02.65 Hand.close @15:34:03.28 CC.seek :open @15:34:08.05 Forearm.seek :retract @15:34:09.04 Elbow.reconfigure :tight @15:34:09.10 Elbow.seek 1 @15:34:10.88 Elbow.reconfigure :limp @15:34:10.93 Forearm.seek 1 @15:34:11.93 Elbow.hold @15:34:12.00 Elbow.reconfigure :tight @15:34:12.05 Hand.open @15:34:12.77 Forearm.force -20 @15:34:12.83 Forearm.seek 1 + 309 counts @15:34:13.67 Forearm.reconfigure :tight @15:34:13.72 Forearm.seek 1 + 450 counts @15:34:13.99 SE.seek :down @15:34:15.13 Forearm.reconfigure :loose @15:34:15.18 Forearm.seek :retract @15:34:16.50 SE.stop @15:34:16.54
Starting finalPurge @15:34:20.08 delay 1 second @15:34:21.10 PRV.dial :waste2,avoiding: :ab2 @15:34:22.94 PTV.dial :PRVmixing @15:34:24.25 PSV.dial :mixloop @15:34:25.30 PS.seek :empty @15:34:36.50
Waiting >20 seconds for psFlush to relinquish FlushPuck @15:34:51.70 PSR.select :air @15:34:51.72 PSV.dial PSR @15:34:52.77 Pulling 0.5ml of PSR.flush @15:34:52.78 PSR.select :flush @15:34:52.90 PS.seek 0.5ml @15:34:58.93 delay 5 seconds @15:35:03.95 PSR.select :air @15:35:04.07 PS.seek 2.5ml @15:35:25.42 delay 1 second @15:35:26.44 PBV.dial :waste1 @15:35:26.82 PTV.dial :mixing @15:35:27.56 PSV.dial :mixloop @15:35:28.62 PS.seek :empty @15:35:55.11 PSV.dial PSR @15:35:56.14 PSR.select :flush @15:35:56.27 PS.seek 1ml @15:36:07.30 PSR.select :air @15:36:07.42 PS.seek 2.5ml @15:36:23.64 PSV.dial PTV @15:36:24.39 PTV.dial :puck @15:36:25.13 PBV.dial :waste1 @15:36:25.32 PS.seek 0ml @15:36:51.70 PSV.dial PSR @15:36:52.45 PSR.select :flush @15:36:52.58 PS.seek 0.5ml @15:36:58.54 PSR.select :air @15:36:58.67 PS.seek 2.5ml @15:37:19.98 PSV.dial PTV @15:37:20.73 PBV.dial PSV @15:37:21.29 PS.seek 0ml @15:37:47.70 PSV.dial PBV @15:37:48.72 PS.seek 2.5ml @15:38:15.01 PSV.dial :waste1 @15:38:15.77 PS.seek 0ml @15:38:42.17 PSV.dial PSR @15:38:42.64 PSR.select :air @15:38:42.66 PS.seek 2.5ml @15:39:09.11 PSV.dial PTV @15:39:09.84 PBV.dial PSV @15:39:10.03 PS.seek 0ml @15:39:36.54 PSV.dial PBV @15:39:37.02 PS.seek 2.5ml @15:40:03.35 PSV.dial :waste1 @15:40:04.10 PS.seek 0ml @15:40:30.39 PSV.dial PSR @15:40:30.86 PSR.select :flush @15:40:30.98 PS.seek 1ml @15:40:41.95 PSR.select :air @15:40:42.07 PS.seek 2.5ml @15:40:58.29 PSV.dial PTV @15:40:59.03 PTV.dial :puck @15:40:59.11 PBV.dial :waste1 @15:40:59.66 PS.seek 0ml @15:41:26.20 PSV.dial PSR @15:41:26.95 PSR.select :flush @15:41:27.07 PS.seek 0.5ml @15:41:32.95 PSR.select :air @15:41:33.07 PS.seek 2.5ml @15:41:54.29 PSV.dial PTV @15:41:55.17 PBV.dial PSV @15:41:55.72 PS.seek 0ml @15:42:22.10 PSV.dial PBV @15:42:22.56 PS.seek 2.5ml @15:42:48.88 PSV.dial :waste1 @15:42:49.62 PS.seek 0ml @15:43:15.94 PSV.dial PSR @15:43:16.41 PSR.select :air @15:43:16.44 PS.seek 2.5ml @15:43:42.88 PSV.dial PTV @15:43:43.62 PBV.dial PSV @15:43:43.70 PS.seek 0ml @15:44:10.22 PSV.dial PBV @15:44:10.70 PS.seek 2.5ml @15:44:37.47 PSV.dial :waste1 @15:44:38.22 PS.seek 0ml @15:45:04.76
Loading FlushPuck into Collection Clamp from Processing Clamp @15:45:04.95 Elbow.seek PC @15:45:07.63 Elbow.reconfigure :loose @15:45:07.74 Forearm.seek PC @15:45:08.70 Hand.close @15:45:09.27 PC.seek :open @15:45:14.03 Forearm.seek :retract @15:45:14.99 Elbow.reconfigure :tight @15:45:15.04 Elbow.seek CC @15:45:18.62 Elbow.reconfigure :loose @15:45:18.72 Forearm.reconfigure :tight @15:45:18.77 Forearm.seek CC @15:45:20.02 CC.seek :closed @15:45:24.60 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.41 @15:45:24.68 Hand.open @15:45:25.33 Forearm.reconfigure :loose @15:45:25.37 Forearm.seek :retract @15:45:26.38 Flushing the Collection Valve::Manifold @15:45:26.50 CSV.dial CSR @15:45:26.93 Pulling 4.999ml of CSR.flush @15:45:26.94 CSR.select :flush @15:45:27.06 CS.seek 5ml @15:45:40.88 delay 5 seconds @15:45:45.90 CSR.select :air @15:45:46.07 CS.reconfigure :fast @15:45:46.12 CS.seek 7.5ml @15:45:52.98 CSV.dial :waste @15:45:54.71 CS.reconfigure :default @15:45:54.76 CS.seek 0ml @15:46:15.01 Flushing lower manifold's waste1 port @15:46:15.13 CSV.dial :flush @15:46:15.70 CS.reconfigure :fast @15:46:15.75 CS.seek 5ml @15:46:28.54 delay 5 seconds @15:46:33.56 CSV.dial :air @15:46:34.21 CS.seek 6.999ml @15:46:39.82 CSV.dial CTV @15:46:39.85
CTV.dial CSV @15:46:41.21 CBV.dial :waste1 @15:46:41.97 CS.seek 0ml @15:46:58.73 Flushing lower manifold's waste2 port @15:46:58.85 CSV.dial :flush @15:46:59.59 CS.seek 5ml @15:47:12.42 delay 5 seconds @15:47:17.43 CSV.dial :air @15:47:17.96 CS.seek 7ml @15:47:23.60 CSV.dial CTV @15:47:23.63
CTV.dial CSV @15:47:24.08 CBV.dial :waste2 @15:47:25.14 CS.seek 0ml @15:47:41.88 Flushing lower manifold's waste2b port @15:47:42.00 CSV.dial :flush @15:47:42.75 CS.seek 5ml @15:47:55.54 delay 5 seconds @15:48:00.56 CSV.dial :air @15:48:01.09 CS.seek 6.999ml @15:48:06.63 CSV.dial CTV @15:48:06.66
CTV.dial CSV @15:48:07.12 CBV.dial :waste2b @15:48:08.14 CS.seek 0ml @15:48:24.91 Disinfecting external Intake with kill @15:48:25.04 CSV.dial CSR @15:48:25.48 Pulling 8ml of CSR.kill CSR.select :kill @15:48:25.60 CS.seek 8ml @15:48:45.66 delay 5 seconds @15:48:50.68 CSR.select :air @15:48:50.80 CS.seek 10ml @15:48:56.45 CSV.dial CBV @15:48:56.48
CBV.dial CSV @15:48:57.54 CTV.dial :intake @15:48:58.32 Intake.select :open @15:48:58.87 CS.seek 2ml @15:49:18.07 Intake.select :close @15:49:18.18 CTV.dial :air @15:49:18.93 CS.reconfigure :default @15:49:18.98 CS.seek 0ml @15:49:24.94 CSV.dial CSR @15:49:25.96 Pulling 5ml of CSR.flush @15:49:25.97 CSR.select :flush @15:49:26.09 CS.seek 5ml @15:49:39.66 delay 5 seconds @15:49:44.68 CSR.select :air @15:49:44.80 CS.seek 10ml @15:49:58.44 CSV.dial CBV @15:49:59.45 CS.seek 0ml @15:50:26.07 Garaging FlushPuck in Collection Clamp @15:50:26.14 Elbow.reconfigure :tight @15:50:26.19 Elbow.seek CC @15:50:26.43 Elbow.reconfigure :loose @15:50:26.53 Forearm.seek CC @15:50:27.49 Hand.close @15:50:28.14 CC.seek :open @15:50:32.78 Forearm.seek :retract @15:50:33.79 Elbow.reconfigure :tight @15:50:33.84 Elbow.seek :garage @15:50:36.77 Elbow.reconfigure :loose @15:50:36.87 Forearm.reconfigure :tight @15:50:36.92 Forearm.seek :garage @15:50:37.97 Hand.open @15:50:38.70 Forearm.reconfigure :loose @15:50:38.74 Forearm.seek :retract @15:50:39.73 Completed [initialPurge; da [50, 50] #da and wsr are run simultanelously] begun 13:58:50 @15:50:39.78 Asleep after completing DA_WCR_011024 assays @15:50:39.80 GoodNews.email "{1 => 4,\n 2 => 0,\n 3 => 0,\n 4 => 0,\n 5 => 0,\n 6 => 0,\n 7 => 0}\nCTD data unavailable\nCan@15:48:05, 26% flow, 22.3C, 14% humidity, 15.1psia, 13.656V, 0.447A, 93.506Ah, 6.11W\nSampled 50.0ml",Subject: "Asleep after completing DA_WCR_011024 assays"