@01:00:00.00CET01-Jan-70 <quick> Simulated gateway v3.24 for core bus
Copyright 2017 MBARI
/home/esp/esp2/type/shallow/mer/configure.rb

Configuration for MFB3 by preston@mbari.org
/home/esp/esp2/type/mfb/MFB3/configure.rb
Polling Can every 10:00
Loading /home/esp/esp2/mission/18tun.rb mission
Preparing to sleep until +0:00:15
<shutdown> Shutdown
<quick> Asleep  til +0:00:15
Continuing at 01:00
@01:00:16.50 All dwarf controllers running firmware version 3.99
@01:00:31.57 Phase [coreNegNTCphase]
==================>  STARTED coreNegNTC (phase #1)  <===
Starting initialPurge
Loading FlushPuck into Collection Clamp from FlushPuck::Garage
@01:00:48.17CET01-Jan-70 Flushing the Collection Valve::Manifold
@01:00:49.67 Pulling 5ml of CSR.flush
@01:01:34.75 Purging external Intake
@01:02:00.71 Flushing upper manifold
@01:03:23.16 Garaging FlushPuck in Collection Clamp
@01:03:36.67 Starting mfb
pcrBAC wcr sampling at most 1500ml
Loading sh1 puck from tube 2 into Collection Clamp
<readyMFB> Readying the MFB
Homing MFB Syringe
@01:03:51.39 <quick> skipping prime and sample collection
Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds)
Calibrating pressure and temperature sensors
@01:04:48.76 BubblePoint: Intake @14.7psia, Outlet @14.7psia
@01:05:09.06 Evacuated: Intake @14.7psia, Outlet @14.7psia
@01:05:37.12 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds)
@01:06:23.38 BubblePoint: Intake @14.7psia, Outlet @14.7psia
@01:06:43.68 Evacuated: Intake @14.7psia, Outlet @14.7psia
@01:07:11.73 Lysing: Adding 0.5ml lysis
@01:07:15.69 Pulling 1ml of CSR.lysis
@01:07:50.15 Pulling 0.5ml of CSR.lysis
@01:08:13.72 Heating sh1 puck from tube 2 in Collection Clamp to 85C for 5:00
@01:15:07.72 Collection Puck Heater at 85C after lysing
@01:15:51.78 Adding 1.0ml diluent to lysate
@01:15:52.53 NameError in quick -- undefined local variable or method `diluentVol' for main:Object
Waiting to Resume from Checkpoint "Adding 1.0ml diluent to lysate"
@01:00:00.00CET01-Jan-70 <quick> Simulated gateway v3.24 for core bus
Copyright 2018 MBARI
/home/esp/esp2/type/shallow/mer/configure.rb

Configuration for MFB3 by preston@mbari.org
/home/esp/esp2/type/mfb/MFB3/configure.rb
Polling Can every 10:00
Loading /home/esp/esp2/mission/18tun_postDeploy.rb mission
Preparing to sleep until +0:00:15
<shutdown> Shutdown
<quick> Asleep  til +0:00:15
Continuing at 01:00
@01:00:16.50 All dwarf controllers running firmware version 3.99
@01:00:31.57 Phase [postDeployPhase]
==================>  STARTED postDeploycoreNegNTC (phase #1)  <===
Starting mfb
pcrBAC wcr sampling at most 0ml
Loading sh1 puck from tube 4 into Collection Clamp
<readyMFB> Readying the MFB
Homing MFB Syringe
@01:00:49.88CET01-Jan-70 <quick> skipping prime and sample collection
Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds)
Calibrating pressure and temperature sensors
@01:01:47.26 BubblePoint: Intake @14.7psia, Outlet @14.7psia
@01:02:07.56 Evacuated: Intake @14.7psia, Outlet @14.7psia
@01:02:35.61 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds)
@01:03:21.87 BubblePoint: Intake @14.7psia, Outlet @14.7psia
@01:03:42.18 Evacuated: Intake @14.7psia, Outlet @14.7psia
@01:04:10.23 Lysing: Adding 1.5ml lysis
@01:04:14.18 Pulling 1ml of CSR.lysis
@01:04:22.81 <mwj> Session Begun
-> abort! "something wrong with mission"
CmdServer::Client::Error in mwj -- Aborting Mission: something wrong with mission
Storage::Warning in quick -- Exiting with Collection Clamp holding Puck.from 4, :sh1
Session Ended
@01:00:00.00CET01-Jan-70 <quick> Simulated gateway v3.24 for core bus
Copyright 2018 MBARI
/home/esp/esp2/type/shallow/mer/configure.rb

Configuration for MFB3 by preston@mbari.org
/home/esp/esp2/type/mfb/MFB3/configure.rb
Polling Can every 10:00
Waiting for espclients...
Log::Locked in trapHandler -- Another process is already writing to /var/log/esp/quick.log
<bkha> Session Begun
-> showlog
-> abort!
CmdServer::Client::Error in bkha -- Attempt to Abort Mission without specifying a reason!
-> abort! "test"
CmdServer::Client::Error in bkha -- Aborting Mission: test
Session Ended
@01:00:00.00CET01-Jan-70 <quick> Simulated gateway v3.24 for core bus
Copyright 2018 MBARI
/home/esp/esp2/type/shallow/mer/configure.rb

Configuration for MFB3 by preston@mbari.org
/home/esp/esp2/type/mfb/MFB3/configure.rb
Polling Can every 10:00
Waiting for espclients...
<bkha> Session Begun
<mwj> Session Begun
<bkha> -> # simulerer lige
-> execute "18tun_postDeploy.rb"
Preparing to sleep until +0:00:15
<shutdown> Shutdown
<bkha> Asleep  til +0:00:15
Continuing at 01:00
<mwj> -> exit
Session Ended
@01:00:16.50 <bkha> All dwarf controllers running firmware version 3.99
@01:00:31.57 Phase [postDeployPhase]
==================>  STARTED postDeploycoreNegNTC (phase #1)  <===
Starting mfb
pcrBAC wcr sampling at most 0ml
Loading sh1 puck from tube 4 into Collection Clamp
<readyMFB> Readying the MFB
Homing MFB Syringe
@01:00:49.88CET01-Jan-70 <bkha> skipping prime and sample collection
Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds)
Calibrating pressure and temperature sensors
@01:01:47.26 BubblePoint: Intake @14.7psia, Outlet @14.7psia
@01:02:07.56 Evacuated: Intake @14.7psia, Outlet @14.7psia
@01:02:35.61 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds)
@01:03:21.87 BubblePoint: Intake @14.7psia, Outlet @14.7psia
@01:03:42.18 Evacuated: Intake @14.7psia, Outlet @14.7psia
@01:04:10.23 Lysing: Adding 1.5ml lysis
@01:04:14.18 Pulling 1ml of CSR.lysis
@01:04:48.64 Pulling 1.5ml of CSR.lysis
@01:05:18.72 Heating sh1 puck from tube 4 in Collection Clamp to 85C for 5:00
@01:12:12.72 Collection Puck Heater at 85C after lysing
@01:12:55.40 Recovering Lysate into Collection syringe
@01:13:37.17 Lysing Complete
Storing sh1 puck in Collection Clamp to tube 3
@01:13:50.63 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation
@01:13:53.78 Tranferring lysate to PRV
@01:13:54.95 Beginning samplePassC
Loading FlushPuck into Collection Clamp from FlushPuck::Garage
@01:14:11.57 Pulling 1.5ml of PSR.flush
@01:17:30.50 Garaging FlushPuck in Collection Clamp
@01:17:44.00 Connecting PS to CS to transfer sample
Loading lyfil puck from tube 4 into Collection Clamp
@01:24:16.22 Storing lyfil puck in Collection Clamp to tube 3
@01:24:23.77 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation
@01:24:38.29 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation
@01:25:06.23 Loading FlushPuck into Collection Clamp from FlushPuck::Garage
@01:25:21.35 Clearing PML for modified lysate
@01:26:29.99 mixing lysate with speDil
@01:30:22.88 Pushing modified lysate into AIV Injection Loop
@01:35:23.76 <MFBPCR> Extracting Nucleic Acid from AIV
<bkha> Beginning samplePassD
@01:35:24.89 Pulling 1.5ml of PSR.killMFB
@01:35:43.97 <MFBPCR> primeLysateINT
@01:36:57.16 <bkha> Pulling 1.5ml of PSR.flush
@01:37:19.64 <MFBPCR> Lysate primed!
@01:38:29.44 <bkha> Pulling 1.5ml of PSR.flush
@01:39:03.24 <MFBPCR> Solid Phase Extraction Heater at 55C
@01:40:01.71 <bkha> Pulling 1.5ml of PSR.flush
@01:40:39.75 <MFBPCR> rinseLysateLineINT
@01:43:43.36 ethanol wash
@01:43:57.44 Pulling 0.4ml of Ethanol Wash
@01:44:24.58 <bkha> Cleaning Collection Syringe
@01:44:24.96 Pulling 4ml of CSR.flush
UserAbort in bkha -- 
-> abort! "test"
CmdServer::Client::Error in bkha -- Aborting Mission: test
-> abort! "test"
CmdServer::Client::Error in bkha -- Aborting Mission: test
Storage::Warning in quick -- Exiting with Collection Clamp holding FlushPuck
Session Ended
@01:00:00.00CET01-Jan-70 <quick> Simulated gateway v3.24 for core bus
Copyright 2018 MBARI
/home/esp/esp2/type/shallow/mer/configure.rb

Configuration for MFB3 by preston@mbari.org
/home/esp/esp2/type/mfb/MFB3/configure.rb
Polling Can every 10:00
Loading /home/esp/esp2/mission/TotalWCRTest.rb mission
Preparing to sleep until +00:00:10
<shutdown> Shutdown
<quick> Asleep  til +00:00:10
Continuing at 01:00
@01:00:11.50 All dwarf controllers running firmware version 3.99
@01:00:26.57 Phase [wcrPhase2]
==================>  STARTED WCRSphase (phase #1)  <===
Starting stand-alone bac WCR
bac wcr sampling at most 2000ml
Whole Cell Archive sampling 2000ml
Loading wcr puck from tube 2 into Collection Clamp
@01:00:41.33CET01-Jan-70 collectSample 2000ml w/bubble point of 55psi
Priming 7ml
@01:00:41.40 Calibrating pressure and temperature sensors
@01:01:24.60 Sampling 2000.0ml
@03:07:51.14 Sampled  2000.0ml
Drying puck before fixing for 4:00
@03:09:01.74 Pumping wcr puck from tube 2 up to 55psi...
@03:10:30.99 Fix #1 of 2 (with rnal) for 2:00
@03:10:31.36 Pulling 2ml of CSR.rnal
@03:10:50.72 Pumping wcr puck from tube 2 up to 55psi...
@03:14:21.09 Fix #2 of 2 (with rnal) for 2:00
@03:14:21.47 Pulling 2ml of CSR.rnal
@03:14:40.82 Pumping wcr puck from tube 2 up to 55psi...
@03:18:11.19 Storing wcr puck in Collection Clamp to tube 1
@03:18:24.66 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation
@03:18:27.81 Starting finalPurge
Loading FlushPuck into Collection Clamp from FlushPuck::Garage
@03:18:42.93 Flushing the Collection Valve::Manifold
@03:18:43.30 Pulling 5ml of CSR.flush
@03:19:28.38 Flushing lower manifold's waste1 port
@03:19:54.72 Flushing lower manifold's waste2b port
@03:20:20.30 Flushing upper manifold
@03:21:44.26 Disinfecting external Intake with mfbkill
@03:21:45.76 Pulling 8ml of CSR.mfbkill
@03:22:20.19 Flushing lower manifold's waste1 port
@03:22:21.32 Pulling 4ml of CSR.mfbkill
@03:22:55.69 Flushing lower manifold's waste2b port
@03:22:56.06 Pulling 4ml of CSR.mfbkill
@03:23:29.67 Killing upper manifold
@03:23:30.05 Pulling 4ml of CSR.mfbkill
@03:24:22.67 Flushing upper manifold
@03:25:44.75 Flushing lower manifold's waste1 port
@03:26:10.71 Flushing lower manifold's waste2b port
@03:26:36.29 Disinfecting external Intake with kill
@03:26:36.66 Pulling 8ml of CSR.kill
@03:27:12.23 Pulling 5ml of CSR.flush
@03:28:12.40 Garaging FlushPuck in Collection Clamp
@03:28:25.90 Completed [wcrPhase2] begun 01:00:26
Asleep after completing TotalWCRTest assays
Mission runtime was 25.409265 seconds